LOCUS       DAORKP010000001        41585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7_length_41585_cov_8.873007, whole genome shotgun sequence.
ACCESSION   DAORKP010000001 DAORKP010000000
VERSION     DAORKP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 41585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_41585_cov_8.873007"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1538)
                     /locus_tag="PLN75_00005"
     CDS             complement(222..1538)
                     /locus_tag="PLN75_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRG02526.1"
                     /translation="MIKENVLKKIEQREIVVGVVGLGYVGLPLAVEKAKAGFKTIGFD
                     VQEAKVDMVNAGHNYIGDVVDDDLKEIVGKGMLSATNDFSFIRDVDFVAICVPTPLDM
                     YQQPDISYVKSSTESIARYMKKGTMVVLESTTYPGTTEELVKPILEAGSGLVCGEDFY
                     LGFSPERVDPGNLIYKTKNTPKVVGAIGDEALEVIAAMYRAVLDSDVHTVSSPAIAEM
                     EKILENTYRNINIGLVNELAMLCHKMNINLWEVIDAAKTKPYGFQAFYPGPGLGGHCI
                     PLDPYYLSWKAREFGFHTSMIESSMMINDRMPEYCMERASVILNRYKKSLSASKILVL
                     GVAYKQDIDDYRESPALAVIDILKGMGAEVLFFDPYISEYKYKGKIHQGEKEMTAELL
                     QSADLVMVTAAHTNVDYDFVQQQATFIFDTKNAMKAVKNRENIELL"
     gene            complement(1774..2838)
                     /gene="rfbB"
                     /locus_tag="PLN75_00010"
     CDS             complement(1774..2838)
                     /gene="rfbB"
                     /locus_tag="PLN75_00010"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRG02527.1"
                     /translation="MKKNAKNILITGGAGFIGNHVVRLFVNQYPDYRIINLDKLTYAG
                     NLANLKDIENAPNYRFVKADICDFEQMLQIFKTYEVTDVIHLAAESHVDRSIKDPFTF
                     AQTNVMGTLSLLQAAKEMWKEDYAAHRFYHISTDEVYGALEFDGSMFTETTSYDPHSP
                     YSASKASSDHFVRAFHDTYGLNVIISNCSNNYGPYQFPEKLIPLFINNIRHEKPLPVY
                     GKGENVRDWLYVVDHARAIDVIFHRGKPGDTYNIGGFNEWKNIDLIKVMIKTVDKLLG
                     NPEGHSLGLITYVTDRAGHDLRYAIDSTKLKNELGWEPSLQFEEGIEKTVKWYLENQE
                     WMDNVTSGEYEKYYENMYGK"
     assembly_gap    3136..3235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3495..4343)
                     /gene="rfbD"
                     /locus_tag="PLN75_00015"
     CDS             complement(3495..4343)
                     /gene="rfbD"
                     /locus_tag="PLN75_00015"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRG02528.1"
                     /translation="MSKKILVTGANGQLGSELRVLSVNSNHEFLFTDVAELDITNREA
                     VDNYVSEHGAEIIVNCAAYTNVDKAEDDTEMADNINHLAVKNLAEVSAKYKLILIHIS
                     TDYVFDGSKNTPYLETDATAPLGVYGHTKLDGEKAVTDSGCDYLIIRTSWLYSSFGNN
                     FVKTIRRLTAEKDSLKVVFDQVGTPTYAGDLAQAILKIIDSGKYADNAGIYHFSNEGV
                     CSWYDFAIEIRNLSGNLCDIQPCHSDEFPSKVKRPNFSVLDKTKIKETFGLKIPYWKV
                     SLREMT"
     gene            complement(4336..4908)
                     /gene="rfbC"
                     /locus_tag="PLN75_00020"
     CDS             complement(4336..4908)
                     /gene="rfbC"
                     /locus_tag="PLN75_00020"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRG02529.1"
                     /translation="MKIIDTEIAEVKILEPDVYGDHRGYFLESFSQREFEEKVFKTEF
                     VQDNESKSRFGVLRGLHFQKAPYAQSKLVRVVQGSVLDVAVDIRKGSPTFGKYVAAEL
                     NDENKRQMFVPRGFAHGFVVLSETVVFQYKCDNYYAPQSEAGIAWDDAALGIDWKIPA
                     AEILLSEKDRNNPLLSEADVLFDYSINLYE"
     gene            complement(5145..6044)
                     /gene="rfbA"
                     /locus_tag="PLN75_00025"
     CDS             complement(5145..6044)
                     /gene="rfbA"
                     /locus_tag="PLN75_00025"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRG02530.1"
                     /translation="MKGIVLAGGSGTRLYPITKGISKQLIPIFDKPMVYYPISALMLA
                     GIRDILIISTPADLPGFRRLLGDGSDYGVNFEYAEQPSPDGLAQAFIIGEKFIGDDAA
                     CLVLGDNIFYGHGFPTMLRNAAKDAAENNLATVFGYWVNDPERYGVAEFDNDGKVISI
                     EEKPKEPKSNYAVVGLYFYPNKVVDVAKNIKPSARGELEITTVNQEFLKDEQLKVQLF
                     GRGFAWLDTGTFDSLAEASTFIEVLEKRQGLKISCLEEIAWRNGWITDERLRQIAQGM
                     IKNEYGQYLMGLLNSAPSISPKE"
     gene            complement(6093..6731)
                     /locus_tag="PLN75_00030"
     CDS             complement(6093..6731)
                     /locus_tag="PLN75_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02531.1"
                     /translation="MRKIVFIVGILFSTLLVSAQTAGNDGFYPGWYVGGNLGTSWLAG
                     ERIDNFSNNPDSRYSLGRNSGSMFRAEIGYNYSPVWGIRGFAGFTQNHWPEATLYYKE
                     VISFASKYVTGDVMMNISNYLEGQNLARSFDFLAFAGTGFHFRNRMTRNGAGLFSMIG
                     RLGAQTNFILNEYMDLKAIFELNIVDDNYNGFEMGSKLDIMPALSVGITYYL"
     gene            7124..7957
                     /locus_tag="PLN75_00035"
     CDS             7124..7957
                     /locus_tag="PLN75_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG02532.1"
                     /translation="MKKINNFYFNVKKDEHKRLRIDNTKKFYNVVFIDLGELAIFTAR
                     VIRQFVSPPYEHRELIRQAYVIGNKSVAMVALTGLIMGLVLTMQTQPTLVQFGVQSLI
                     PGMVAVSMFREVGPVVTGLICAGKISSGIGAEIGAMKVTEQIDAMEVSGTKPLSFVVA
                     TRVLATTVMVPLLVFFSDAFSLIGSYLGVNLFESMSFELFMKNAFDMLGFMDVIPATI
                     KSVVFGFFIGLIGAYKGYQAGLGTESVGQAANSAVVNASLSIFIIDLVAVLITNLLTG
                     N"
     gene            7959..8678
                     /locus_tag="PLN75_00040"
     CDS             7959..8678
                     /locus_tag="PLN75_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG02533.1"
                     /translation="MIEVKDLYKAFGENVILNGVGFHLLKGENLGILGKSGSGKSVLT
                     KCIVRLIEPDFGEIKVLGKDILKMSLNELNEIRKDIGYLFQGGALYDSMNVRENLEFP
                     IRRTQMASNKHEVNERVEQALRSVGLLDAINKMPAELSGGMKKRIGLARALILRPKVI
                     LYDEPTTGLDSVTSGEISELILKVQEEYNASSIIITHDMKCAKIATDKIKILENGTFL
                     TEGTYDELSKSTDRQIRAFFM"
     gene            8734..9699
                     /locus_tag="PLN75_00045"
     CDS             8734..9699
                     /locus_tag="PLN75_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRG02534.1"
                     /translation="MKNQTNKALRVGLFVFTALAIFITIIYLIGSKDNLFKTKTTITT
                     SFNDIRGVVVGNNVRFSGINVGKVSDINVVSDKEVVLELSIVKEYARFIYKDALVEIN
                     QDGLMGSKLINITSGHSSAGMIEEGTHLKGKEGIDIENMLYEVHDILVEAGDAVASLK
                     SIAGKIDSGEGDLAKLVNDDVLSTELTATTRQLNTTLATVEQITKNMTTGEGDIARLI
                     NSNELTSQAHQLLGNLNEAVEKTHRVAENLETTSRQINYGEGAVNLLLNNKNTAQNID
                     TTILKLQNSLNEFDKTARAVQNSWLIQLFNRKKLKDKKTAKDSIK"
     gene            complement(9755..11533)
                     /locus_tag="PLN75_00050"
     CDS             complement(9755..11533)
                     /locus_tag="PLN75_00050"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRG02535.1"
                     /translation="MTQINGRISALREAMKKAGVAACIIPGTDPHASEYIADFWKERE
                     WISGFDGSAGTVALTHQKAGLWTDSRYFLQGAQQLEGTEIQLMKQGLPETLEIIPWLS
                     TELRKGEKVAVSAEMFSVNGYQNMKTELALSDIELVSIDLMKDVWIDRPELPKNPFFI
                     FDKKYTGKSVGEKLDELRHEMKKLYADVFVMSALDDIAWLFNIRGNDVDYNPVVITYA
                     LIDEKSATLFISSEKITTETKKYLDKNGIIVKDYFNIHEELQKIPKSKAVLVDAGKLN
                     QSLFEAIPAECIKRKAMSPVFRLKSVKNKTEMRGVRNAMIKDGVALTRFFMWFEKSLK
                     NEKLTELSISDKLRSFREDQEGFRGESFGTIAGYAGHGAIVHYKATPESDAVIKNVGI
                     LLLDSGGQYLDGTTDITRTIALGKPSKKEKRDFTMVLKGHIQLALAKFPVNTRGSQLD
                     ILARKAMWDEGINYGHGTGHGIGHFLNVHEGPQNIRMDENPVTLQPGMMISNEPGLYR
                     TGEYGIRIENLVQVIPAEKTEFGQFLKFETLTLCYIDTRLVEKDMLTDNERKWLNDYH
                     LRVCKTLCPNLTEKERKWLSRKTEAI"
     gene            11874..>12871
                     /locus_tag="PLN75_00055"
     CDS             11874..>12871
                     /locus_tag="PLN75_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRG02536.1"
                     /translation="MDLINQPLRIKNTVLKNRIGMSPMCMYSAADGFATDWHLAHYGA
                     RATGGTALIIQEATAVTPEGRITPYDLGIWKDEHIEQLKKITGFISSQGAVPAIQLAH
                     AGRKASHDKPANGGKFLSPEHGGWQTVAPSAVPFSPETNVPTALTEYGIYQIIELFAK
                     AARRAVKAGYRIIEIHAAHGYLIQEFMSPLSNHRNDEYGGSFENRIRLLLQVVEAVKR
                     VIPDEMPLFVRVSASEWTEGGWTIDETVKLTEILMEKGVDLMDCSSGGNIADAKMAPG
                     PLYQVPFAEAVHNTGIKTAAVGLITTPQQVNSILEKGQADLVLLGRELLRNPYFAL"
     assembly_gap    12872..12971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13010..15901
                     /gene="uvrA"
                     /locus_tag="PLN75_00060"
     CDS             13010..15901
                     /gene="uvrA"
                     /locus_tag="PLN75_00060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRG02537.1"
                     /translation="MHLPDTTENVDVIGARVHNLKNIDVSIPRDSLTVITGLSGSGKS
                     SLAFDTIFAEGQRRYIETFSAYARSFLGNMERPDVDKITGLSPVISIEQKTTNKNPRS
                     TVGTTTEIYDFLRLLFARAGVAYSYLSGEKMVKYTDEQIVNLILQEYEGKKIYLLAPV
                     VRGRKGHYRELFENIRKKGYIHVRVDGELCEITHNMKVDRYKIHDIEIVIDRLIITAK
                     DRKRLLDSMQKAMQQGNGVIMIAPPLPEKASSNSPKGGENIPHSAELDGARYFSRKLM
                     CPSTGISYDEPAPHHFSFNSPHGYCPRCKGLGFVNEIDMEKIIPDKSLNIHNGGIAPL
                     GKHKNSMIFWQIEAILEKYECTLKTPIVDIPDDAIDEIMNGTDERLNIKNQSLGNSNY
                     FLNYDGILKYIEMQQENTASATEQKWANQYSKTSKCPECNGQRLRKESLFFRIHDKNI
                     AELAEMDMLELNEWLKNVEQFMSDKQKTIASEILKELKSRLQFLLDVGLNYLAMNRPS
                     LSLSGGESQRIRLATQIGSQLVNVLYILDEPSIGLHQRDNERLITSLKKLRDTGNSVI
                     VVEHDKDMMLAADYIVDLGPRAGRLGGELVFAGTPDEILLSDTLTADYLTGKKKIEIP
                     ESIRKGNGKFLTIKGARGNNLKNVDVTFPLGKMICVTGVSGSGKSSLINETLQPILSQ
                     HFYRSLKDPLDYDTVEGIENLDKVVQVDQSPIGRTPRSNPATYTGVFSDIRNLFASLP
                     EAKIRGYKTGRFSFNTAGGRCETCGGNGYKTIEMNFLPDVFVPCETCNGKRYNRETLE
                     VRFKGKSIADVLNMTINQAVEFFENQQSILNKIKTLQDVGLGYVKLGQSSTTLSGGES
                     QRVKLATELSKKDTGKTLYILDEPTTGLHFEDIRVLLKVLNRLADKGNTVIIIEHNLD
                     VIKVADYIIDIGPEGGNRGGYIMCAGTPQEVASHAHSLTGKFLKKEFQQ"
     gene            16014..16220
                     /locus_tag="PLN75_00065"
     CDS             16014..16220
                     /locus_tag="PLN75_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02538.1"
                     /translation="MNSTIKLIIYAISTFLVFLLLTWILRLMAGKLPIENGILGVFKN
                     SDLLLGLVVAVAVTFSHIQKRKLK"
     gene            16242..17555
                     /locus_tag="PLN75_00070"
     CDS             16242..17555
                     /locus_tag="PLN75_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRG02539.1"
                     /translation="MTKISDYIIRFLLGNENAGFAKHIGYYSDKDDYSGCKLVIRRSN
                     FFDPDIYGTTQSYPALPLSLWKDIPLLFGSPETKKSGNTLILHADIIASTFFLISRYE
                     EVILRDERDQHGRFPGKKSLAFKAGFLQRPLVDEYGAMLRKLLRETGYNITEPSEKFR
                     KIYLTHDVDQLAHYRNFRGMAGAVSRFFKNPYQTGKAVKSYFGGIGNDPWYTFPWFFD
                     LAEELRKEKPESNCESIVFFKTGGGDLMTDKPLHDVRDKDFNRLFSLCKQFGVKIGLH
                     PSYQAGMNPNYIQKEKKILDETTDQQTTYSRNHFLSNREPEHMQALIEAGITDDFTMA
                     YADIAGFRLGTSRPVKWINPLTGEITDLTLHPLILMDSTLSDERYMKLRADEAFSYSK
                     KMIDLVKKHNGDLVLLWHNTTVEKHNGQYHRDLYRWMINYLKMNL"
     gene            17558..18676
                     /locus_tag="PLN75_00075"
     CDS             17558..18676
                     /locus_tag="PLN75_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG02540.1"
                     /translation="MSKKAIVCVTNDLTTDQRVSKTCTTLLKCGYQVIETGRLLPDSL
                     EFAPSYTIRRKKLWFNTGALFYAEYNIRLFFYLLFARVDLIFSNDLDTLPAAFVVSEI
                     RRKKLIYDSHEYFTEMPELVGRKKIQATWKFFERAIFPKLKHIITVNHSIADLYAKEY
                     QKEIHVVRNIPPTYNPDVIKSRKELDLPEDKKILILQGTGINIDRGAEEAVMAMKYIE
                     NAVLLIVGRGDVIPALRHIVQEEKLEHKIIFKPKMPFTELRQHTMNADLGLVIDKDTN
                     LNYRFSLPNKLFDYIHSDIPVLASELPEIKNIIDKYDIGYYIPNHSPQAIAETVNRIF
                     ENKSRYQTVKHNTQKAKTELSWEKEELNLIEVINNVNL"
     gene            18703..19599
                     /locus_tag="PLN75_00080"
     CDS             18703..19599
                     /locus_tag="PLN75_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG02541.1"
                     /translation="MLSILIPTYNYNITHLVNELHRQAMNTYVDFEILVMEDGSTQFV
                     HENKAVADLKYCKYTILTKNIGRSAIRNRLADDARFNHLLFLDCDSEVIMPHFIEKYI
                     AFCKENSVVVGGTAYDTGNKNPNFSLRLKYGRLREAKSALEREKDGLYSHFSAFNFLI
                     SKSTFNQIRFDEDIQGYGHEDTLFGHKIAELKCIMHHIDNPLLHRGLDENDIFIRKTE
                     ESTRNLYILYLTGKYPSLEKQSKLLFKFNQFKKKNFVCVLAALFTALQPVLMSNLTSS
                     APSLRLYDLYKLLFLAKISRSK"
     gene            19749..20018
                     /locus_tag="PLN75_00085"
     CDS             19749..20018
                     /locus_tag="PLN75_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRG02542.1"
                     /translation="MNIKPLADRVLVKPAPAEEKTISGIIIPDSAKEKPLKGEILAVG
                     NGTKDEEMIVKVGNTVLYGKYAGTELEWEGEKYLIMRQNDILAII"
     gene            20135..21766
                     /gene="groL"
                     /locus_tag="PLN75_00090"
     CDS             20135..21766
                     /gene="groL"
                     /locus_tag="PLN75_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRG02543.1"
                     /translation="MAKEILFNIDARDQLKKGVDELANAVKVTLGPKGRNVILEKKFG
                     APHITKDGVTVAKEIELEDAYQNMGAQLVKEVASKTGDDAGDGTTTATVLAQSIVNVG
                     LKNVTAGANPMDLKRGIDKAVESVVKNIASQARTVGDNYDKIEQVATISANNDSRIGK
                     LIADAMRKVSKDGVITIEEAKGTETTIEVVEGMQFDRGYISPYFVTNTEQMEVEFEKP
                     QILIYDKKISNLKELLPILEPAVQSGRPILIIAEDVDSEALTTLVVNRLRGSLKICAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGIKLENATLEMLGTAEKITVDKDNTTIVN
                     GAGEKDAIQTRIGQIKSQIASTTSDYDKEKLQERLAKLSGGVAVLYVGAASEVEMKEM
                     KDRVDDALHATRAAIEEGIVPGGGVAYIRSIDTLDNITPDNEDERTGIEIVKRAIEEP
                     LRQIVANAGKEGAVVAQKVREGKADYGYNARTDKYENFFEAGVVDPAKVCRIALENAA
                     SIAGMFLTTECVIVEKKEDNPAPQMPMGGGMGGMM"
     gene            21955..22989
                     /locus_tag="PLN75_00095"
     CDS             21955..22989
                     /locus_tag="PLN75_00095"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRG02544.1"
                     /translation="MKIGILSSGGDCPGINATIRGVGKTAISKYGMEVIGIQNGFSGL
                     LYKDIIPLTEGSLSGILTLGGTILGTAREKEFRKILKSPDKKDQEVIKKSYKELGLDC
                     LVCIGGNGTQKTTWQLSELGLNVIGIPKTIDNDVFGTDVTFGFNSAVDIATDAIDRLH
                     STASSHQRVMVIELMGHHAGWITLYAGMAGGADVILLPELGYDLNTVTEVLNRRKKSG
                     KAYSIVAVAEGIVVEGGTDGHHPATYFARKIEELTGIEARETVLGYIQRGGSPSPYDR
                     NLGTLLGGHAAKLINDEKFGRMVSVKGIDVITDVPLKDVAGKLRLVTPDHIMVKQGKR
                     MGISFGKLGL"
     gene            complement(23064..23438)
                     /gene="rplT"
                     /locus_tag="PLN75_00100"
     CDS             complement(23064..23438)
                     /gene="rplT"
                     /locus_tag="PLN75_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRG02545.1"
                     /translation="MPRSVNHVASRKRRKRILSLTRGYFGGRRNVWTVAKNTWEKGLQ
                     YAYRDRKNKKRNFRALWIQRINAAARLEGMSYSRLMGALHKAGIEMNRKVLADLAMNH
                     PEAFSAIVKSVNHTENKAKAVK"
     gene            complement(23547..23741)
                     /gene="rpmI"
                     /locus_tag="PLN75_00105"
     CDS             complement(23547..23741)
                     /gene="rpmI"
                     /locus_tag="PLN75_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRG02546.1"
                     /translation="MPKMKTNSGAKKRFALTGTGKIKRKHAFKSHILTKKTTKQKRNL
                     TQTGLVDSVDVKNVKAMLRV"
     gene            complement(23774..24313)
                     /gene="infC"
                     /locus_tag="PLN75_00110"
     CDS             complement(23774..24313)
                     /gene="infC"
                     /locus_tag="PLN75_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRG02547.1"
                     /translation="MRPRQGQQKEMPNRINEKIKGIKEVRLVGENVEPGIYTIERAMQ
                     IAEDLELDLVEIAPNAAPPVCKIVDYQKFLYQLKKKEKENKAKSTKIDVKEIRFGPQT
                     DDHDYNFKLKHAIKFLQEGDKVKAYVFFKGRSILFKEQGEVLLLRFANDLEEYAKVDQ
                     LPQLEGKRMIIMFSPKKTK"
     gene            24634..25158
                     /locus_tag="PLN75_00115"
     CDS             24634..25158
                     /locus_tag="PLN75_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRG02548.1"
                     /translation="MKKLILLFVVLSMMFSSSLYSQQRVKASYYADKFHGRKTASGKL
                     YNRDSLTCAHKTYPFGTILKVKNPRNDKEIYVKVTDRGPFVKGRSIDLSYAAAKKIGV
                     IGHGISIVDITKTNLTQIPKPGERKMIPDSLNLENIEIRSDSIIIQPDNTKVSNDSIK
                     LALKINIPIKTKTA"
     gene            complement(25203..25703)
                     /locus_tag="PLN75_00120"
     CDS             complement(25203..25703)
                     /locus_tag="PLN75_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRG02549.1"
                     /translation="MGHHQIDRLDKKILQLISKDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFVVDTYKIGYLTCAYIGIVLSDIKKFQSVVAELKKVPEIVECHYAT
                     GKYSMFIKIYAKDNRHLLQIILENLTTIDGVQHTETFQLSLDEIFNRQLSVFDTEEGD
                     DDEIEE"
     gene            complement(25828..26751)
                     /gene="rsgA"
                     /locus_tag="PLN75_00125"
     CDS             complement(25828..26751)
                     /gene="rsgA"
                     /locus_tag="PLN75_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRG02550.1"
                     /translation="MQGLVVKNTGSWYTVKTDDGQLVDCKIRGNLRIRDIRSTNPIAV
                     GDRVEFSSAADGSAVIHEICDRKNYIIRRSSNLSKFSQILAANLDLTALVVTVNYPET
                     SSVFIDRFLATAEAYRVPACIIINKSDRYNVAELEYAKSLKKLYEIIGYPVFLVSALH
                     PETLDELQQFLAGKITLISGNSGVGKSTIISQLIPESTIRVGVISEYHNRGMHTTTFS
                     EMFTLPGGGGIIDTPGIKGFGTIDMSEIEVGHFFREIFEFSHKCRFNNCTHTHEPGCA
                     VLEALENHYISQSRYQSYLSILEDSKNEKYR"
     gene            complement(26867..27427)
                     /gene="frr"
                     /locus_tag="PLN75_00130"
     CDS             complement(26867..27427)
                     /gene="frr"
                     /locus_tag="PLN75_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRG02551.1"
                     /translation="MQDTKAIMQEAEEFMNAAIGYLDEALAHIRAGKANTRILDGIRV
                     DYYGSMVPITNVATVTTPDAKTIAIQPWEKAMIHVLEKAIMNSDVGITPVNNGEVIRL
                     GIPPLTEERRKQLVKQSRSEGEEAKISIRNARREAIEHFKKMVKEGLPEDVEKDAETE
                     VQKIHDKYIRKVDELLLEKEKEIMTV"
     gene            complement(27486..28196)
                     /gene="pyrH"
                     /locus_tag="PLN75_00135"
     CDS             complement(27486..28196)
                     /gene="pyrH"
                     /locus_tag="PLN75_00135"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRG02552.1"
                     /translation="MIKYNRILLKLSGESLAGATKQGIDTLRLKEYATQIKQIADIGV
                     QIGIVIGGGNIFRGLSGATKGFDRVQGDQMGMLATVINSLALNSALQANGTKSRVLTS
                     VRMEPVGEYYSKRYAVECLERGEVVIIAGGTSNPYFTTDTASALRAIEIEADVMLKGT
                     RVDGVYTADPEKDPTAVKFDEITYDEVYNRNLKVMDLTATTMCKENSMPIYVFDMDTV
                     GNLKKVVMGENIGTLVKN"
     gene            complement(28346..28876)
                     /gene="rplQ"
                     /locus_tag="PLN75_00140"
     CDS             complement(28346..28876)
                     /gene="rplQ"
                     /locus_tag="PLN75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRG02553.1"
                     /translation="MRHNKKFNHLGRTASHRHALLSNMAVSLIRHKRIATTLAKAKAL
                     RVYVEPLLTKAKDDTTHSRRTVFSYLQNKEAVTELFRDVAVKIADRPGGYTRILRTGF
                     RLGDNAEMCIIELVDYNENMLKDKTSRKTTRTRRSSKKAAETEAKTEAPKAKTTKVEA
                     KKEEPKAETPAEEKAE"
     gene            complement(28885..29877)
                     /locus_tag="PLN75_00145"
     CDS             complement(28885..29877)
                     /locus_tag="PLN75_00145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRG02554.1"
                     /translation="MAILAFQKPEKVIMLEADDFQGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAINVIHIEGIDHEFASIPGVLDDVTNIILNLKQIRFKQIVEEIENEKVTI
                     TVKGTTTFKAGDLGKYLTGFDVLNPELVICTMDSKASFNIDITISKGRGYVPADEVKL
                     STSEIGVIPIDAIYTPIRNVKYTVENYRVEQVTDYEKLVIEITTDGSIHPKEALKEAA
                     KILIHHFMLFSDEKISLEVPEGEGVEEFDEEILHMRQLLKTKLTDLELSVRALNCLKA
                     ADVTTLGELVSNHRHDLLKFRNFGKKSLTELEEKLESLNLNFGMDVTKYKLDKE"
     gene            complement(29886..30494)
                     /gene="rpsD"
                     /locus_tag="PLN75_00150"
     CDS             complement(29886..30494)
                     /gene="rpsD"
                     /locus_tag="PLN75_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRG02555.1"
                     /translation="MARYTGPKTKIARKFGEPIFGPDKVLSKKNYPPGQHGNSRRRKT
                     SEYGIQLREKQKAKYTYGLLEKQFRNTYEKAKRTKGITGVILIQLLESRLDNVVYRLG
                     FAPTRAGARQLVSHRHITVDGKVVNIASYNVRPGQVVAVREKNKSMEVILNSLSGFNH
                     SKYPWIEYSDSTMSGTFLHIPERDDIPENIKEQLIVELYSKN"
     gene            complement(30645..31034)
                     /gene="rpsK"
                     /locus_tag="PLN75_00155"
     CDS             complement(30645..31034)
                     /gene="rpsK"
                     /locus_tag="PLN75_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRG02556.1"
                     /translation="MAKKTVAAKKRVVKVDGVGQLHVHSSFNNIIVTLANSEGQVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCSKVAFELGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            complement(31089..31469)
                     /gene="rpsM"
                     /locus_tag="PLN75_00160"
     CDS             complement(31089..31469)
                     /gene="rpsM"
                     /locus_tag="PLN75_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRG02557.1"
                     /translation="MAIRIVGVDLPQNKRGEVGLTYIYGIGRSSAKKILEQAGVDINI
                     KVKDWTDDQAAKIREIIGTGFKVEGDLRSETQLNIKRLMDIGCYRGIRHRIGLPLRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            complement(31501..31617)
                     /gene="ykgO"
                     /locus_tag="PLN75_00165"
     CDS             complement(31501..31617)
                     /gene="ykgO"
                     /locus_tag="PLN75_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRG02558.1"
                     /translation="MKVRASIKKRTADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(31709..31927)
                     /gene="infA"
                     /locus_tag="PLN75_00170"
     CDS             complement(31709..31927)
                     /gene="infA"
                     /locus_tag="PLN75_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRG02559.1"
                     /translation="MAKQQAIEQDGTIIEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDRVKVEMSPYDLSKGRISFRYK"
     gene            complement(31931..32716)
                     /gene="map"
                     /locus_tag="PLN75_00175"
     CDS             complement(31931..32716)
                     /gene="map"
                     /locus_tag="PLN75_00175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRG02560.1"
                     /translation="MIYLKSDEEIELLRNSNLIVAKTLAELAKIIAPGVSTLQLNNRA
                     DEFIRDNGGIPNFLGYGGFPGSICTSVNDQIVHGIPSDKVILKDGDIISIDCGVLKDG
                     FNGDSAYTFCVGEVEPEIISLLRATKESLYKGIEQAYEGKRIGDIGAAVQTYCESRGY
                     SVVREMVGHGVGRKLHESPEVPNYGRKGNGVLLKSGMTIAIEPMINLGTRNLIFEKDG
                     WTTRTADKKPSAHFEHSVAIRQGKADILSSFEYIEQVLGDREF"
     gene            complement(32808..34154)
                     /gene="secY"
                     /locus_tag="PLN75_00180"
     CDS             complement(32808..34154)
                     /gene="secY"
                     /locus_tag="PLN75_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRG02561.1"
                     /translation="MKKFIETLKNIWRIEDLRNRLITTIGFVLIYRFGSYVVIPGVDA
                     NALAALKSQTSGGIMALLDMFSGGAFQNASIFALGIMPYISASIVIQLLTIAVPKFQK
                     LQREGESGRTKMNQYTRYLTVLILVLQGPSYMANLNVQVAQAGSSLPGGFLFIATSTI
                     ILMAGSMFVMWLGERITDKGIGNGISFIIMIGIISRLPGALIQEFSSRLADSGGGGLV
                     MFLLEIVILIVVIMFSILLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAAGVMP
                     IIFAQAIMFIPLAIAGYSNSEATQTFWSHFTRYESFWYNLVFALLIIAFTYFYTAITV
                     NPTQMAEDMKRNNGFIPGIKPGKRTAEYLDLIMSRITLPGSFFLAIIAILPAFASILG
                     INQQFAQFFGGTSLLILVGVILDTLQQVESHLLMRHYDGLLKSGRIKGRTGSASAY"
     gene            complement(34159..34605)
                     /gene="rplO"
                     /locus_tag="PLN75_00185"
     CDS             complement(34159..34605)
                     /gene="rplO"
                     /locus_tag="PLN75_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRG02562.1"
                     /translation="MNLSNLTPAAGSVKTRKRIGRGSGSGMGGTSTRGHKGAKSRSGY
                     SRKVGFEGGQMPIQRRLPKFGFKNINRVEFKAINLETLQVIADSNNLTKIGLNELREA
                     GYISKSAMVKILGKGTLTAKLEVEANAFSKSAEEAIVAAGGSVVKI"
     gene            complement(34647..34823)
                     /gene="rpmD"
                     /locus_tag="PLN75_00190"
     CDS             complement(34647..34823)
                     /gene="rpmD"
                     /locus_tag="PLN75_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRG02563.1"
                     /translation="MATIKIQQVRSRIRCPKDQKRTLDALGLKKMHAVVEHEATPQIL
                     GMVAKVHHLVQVIK"
     gene            complement(34837..35343)
                     /gene="rpsE"
                     /locus_tag="PLN75_00195"
     CDS             complement(34837..35343)
                     /gene="rpsE"
                     /locus_tag="PLN75_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRG02564.1"
                     /translation="MNIVKTTNDLELKDRLVAVNRVTKVTKGGRTFSFSAIVVVGNED
                     GIVGWGLGKAGEVTAAIAKGTEAAKKNLIKVPVLNGTIPHEQIAKFGGARVYIEPATN
                     GTGVKAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATILALSELRDANQVAQNRGV
                     SLSTVFNG"
     gene            complement(35362..35706)
                     /gene="rplR"
                     /locus_tag="PLN75_00200"
     CDS             complement(35362..35706)
                     /gene="rplR"
                     /locus_tag="PLN75_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRG02565.1"
                     /translation="MSDKINRRSKIKAHIRTKITGTAERPRLTVFRSNAQIYAQIIDD
                     VKGVTLASASSLGSKDKVTKSEQAAAVGKLIAKNALSAGINEVVFDRNGYLYHGRVKQ
                     LADAAREGGLKF"
     gene            complement(35728..36279)
                     /gene="rplF"
                     /locus_tag="PLN75_00205"
     CDS             complement(35728..36279)
                     /gene="rplF"
                     /locus_tag="PLN75_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRG02566.1"
                     /translation="MSRIGKLPIEIPAGVTVSVKDQTITVKGPKGELNQTVSPNVKVE
                     IEGNECKIIRNEDDKQSRADHGLYRSLINNMVVGVSQGYEKVLELVGVGYRASGQGQV
                     LELSLGYTHPIFLVLPNEVSVETVSERNQNPLIKLRSIDKQLLGQICAKIRSFRKPEP
                     YKGKGVKFRGEVIRRKAGKTAGK"
     gene            complement(36297..36692)
                     /gene="rpsH"
                     /locus_tag="PLN75_00210"
     CDS             complement(36297..36692)
                     /gene="rpsH"
                     /locus_tag="PLN75_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRG02567.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKKEMTRILHEKGYI
                     LNYKFVEEGPQGTIKIALKYDPVNKVNSIKNLKRVSTPGLRQYVGYKDMPRVLNGLGI
                     AILSTSKGVMTNKEAVDLKIGGEVLCYVY"
     gene            complement(36847..37116)
                     /gene="rpsN"
                     /locus_tag="PLN75_00215"
     CDS             complement(36847..37116)
                     /gene="rpsN"
                     /locus_tag="PLN75_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRG02568.1"
                     /translation="MAKESMKAREVKRAAMVAKYAAKREQLKTEGDYEALQAIPKNAS
                     PVRLHNRCKLTGRPKGYMRQFGISRIQFREMASKGLIPGVRKTSW"
     gene            complement(37131..37676)
                     /gene="rplE"
                     /locus_tag="PLN75_00220"
     CDS             complement(37131..37676)
                     /gene="rplE"
                     /locus_tag="PLN75_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRG02569.1"
                     /translation="METASLRKEYKERIVPALIKEFGYSTVMQVPKLEKIVLNQGLGA
                     AVADKKIIETAVNEMTTITGQKAVQTISKKDISNFKLRKKMPIGVRVTLRGERMYEFL
                     ERLVRVALPRIRDFKGIENKLDGHGNYTLGITEQIIFPEINIDSISKLMGMNITFVTT
                     ANTDEEGFALLKEFGLPFKKN"
     gene            complement(37678..37989)
                     /gene="rplX"
                     /locus_tag="PLN75_00225"
     CDS             complement(37678..37989)
                     /gene="rplX"
                     /locus_tag="PLN75_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRG02570.1"
                     /translation="MSKLHIKKGDTVYVNTGVDKGKTGRVLEVLVKKQRAIVEGINLV
                     SKHTKPNAKSPQGGIVKQEAAIHISNLNPAEKGKPVRIGRKLNTEGKLVRVSKKTGEE
                     I"
     gene            complement(38009..38374)
                     /gene="rplN"
                     /locus_tag="PLN75_00230"
     CDS             complement(38009..38374)
                     /gene="rplN"
                     /locus_tag="PLN75_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRG02571.1"
                     /translation="MIQQESRLTVADNSGAKEALCIRVLGGTGKRYATLGDIIVVTVK
                     NVIPSGDIKKGTVSKAVIVRTKKEVRRADGSYIRFDDNACVLLNAAGEIRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            complement(38380..38637)
                     /gene="rpsQ"
                     /locus_tag="PLN75_00235"
     CDS             complement(38380..38637)
                     /gene="rpsQ"
                     /locus_tag="PLN75_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRG02572.1"
                     /translation="METRNLRKVRTGVVSSNKMDKTITVAVKWKEKHPIYGKFVNKTK
                     KYHAHDEKNDCNIGDTVTIMETRPLSKLKRWRLIQINERAK"
     gene            complement(38651..38845)
                     /gene="rpmC"
                     /locus_tag="PLN75_00240"
     CDS             complement(38651..38845)
                     /gene="rpmC"
                     /locus_tag="PLN75_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRG02573.1"
                     /translation="MKTSEIKELTDKELLERLDAEKNHLSRMKMNHAISPLDNPLQIK
                     DIRRNIARFATELRQREINK"
     gene            complement(38858..39286)
                     /gene="rplP"
                     /locus_tag="PLN75_00245"
     CDS             complement(38858..39286)
                     /gene="rplP"
                     /locus_tag="PLN75_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRG02574.1"
                     /translation="MLQPKKTKFRRQQDGRFKGNAQRGNQLAFGSFGIKTLEAKWLTG
                     RQLEAARVAVTRFMQREGQVWIRVFPDKAITRKPADTRQGKGKGNPEGFVAPVTPGRI
                     LFEIEGVSFDIAKEALRLAAQKLPVATKFVVRRDYDYTSK"
     gene            complement(39311..40054)
                     /gene="rpsC"
                     /locus_tag="PLN75_00250"
     CDS             complement(39311..40054)
                     /gene="rpsC"
                     /locus_tag="PLN75_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRG02575.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGNNYGDTILEDSKIRKYLNA
                     RLAKASVSRIVIERTLKLITITVCTARPGIIIGKGGQEVDKLKEELKKITDKEVQINI
                     FEIKRPELDAVIVANNVARQVEGKIAYRRAVKMAIASTMRLGAEGIKIQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGLIGIKVWICRGEVYGKKDLAPNFTS
                     AKETGRGGYHANERSDRGGKGFKKRKNNR"
     gene            complement(40060..40470)
                     /gene="rplV"
                     /locus_tag="PLN75_00255"
     CDS             complement(40060..40470)
                     /gene="rplV"
                     /locus_tag="PLN75_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRG02576.1"
                     /translation="MGQRKKISAEARKEANKTLYFAKLNNVPTSPRKMRLVADMIRGM
                     EVNKALSVLKFSNKEASAKMEKLLKSAIANWETKVEDKAENANLYVSAVYVDSATMLK
                     RLRTAPQGRGYRIRKRSNHVTLYVDTKNTNDNQN"
     gene            complement(40518..40784)
                     /gene="rpsS"
                     /locus_tag="PLN75_00260"
     CDS             complement(40518..40784)
                     /gene="rpsS"
                     /locus_tag="PLN75_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRG02577.1"
                     /translation="MSRSLKKGPFINVKLEKKVLAMNESGKKSVIKTWSRATMISPDF
                     VGHTIAVHNGNKFIPVYVTENMVGHKLGEFAPTRIFRGHGGKKK"
     gene            complement(40807..41583)
                     /gene="rplB"
                     /locus_tag="PLN75_00265"
     CDS             complement(40807..41583)
                     /gene="rplB"
                     /locus_tag="PLN75_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRG02578.1"
                     /translation="MIGTFDTITASAPEKSLVTGTRKSGGRNHDGKMTMRYIGGGHKR
                     KYRVIDFKRNKDGVPATVKAIEYDPNRSARIALLYYADGEKRYILAPNGLQVGQVVLS
                     GAEAAPEVGNALFLQNIPLGTVVHNIELRPGQGASMARSAGTFAQLTSREEKYVIVKL
                     PSGEIRKILSTCKATVGSVGNSDHALERSGKAGRSRWLGRRPRVRGVAMNPVDHPMGG
                     GEGRASGGHPRSRKGLLAKGYRTRYPKNQSNQYIIERRKK"
BASE COUNT        11431 a         8999 c         8281 g        12674 t
ORIGIN      
        1 ccacctaaat atcctaactg aaacagtgtg caaatatggt tttgtcccca ttttccgccg
       61 cctattacac aaattctttt cataaaaagc ccctccaacc tccccgaagg ggaggcttaa
      121 tcttctgatt tatattatat tctattttct aagtttttaa attaataagc tgagatgtct
      181 cctttggttt gcctgcctca ggcggggaga tatatagggt tttataataa ttcaatattt
      241 tcccggtttt tcaccgcttt catcgcattt ttggtatcga aaataaatgt agcctgttgc
      301 tgaacgaaat catagtccac gttggtgtga gcagctgtaa ccatcaccag gtcggcagat
      361 tggagcagtt cggctgtcat ttccttttct ccctgatgaa tttttccctt gtatttgtat
      421 tctgaaatat aaggatcgaa aaacagcact tctgctccca ttcctttcag aatatctatc
      481 actgccagcg ccggactttc gcgataatcg tcgatgtctt gcttgtaagc cacgccgagt
      541 accaatattt tggatgcact caaggatttt ttataccggt tgagaataac gcttgcacgc
      601 tccatgcagt attcgggcat gcggtcgttg atcatcatcg aagattcaat catggaagta
      661 tggaatccga attcacgggc tttccacgaa aggtaatacg gatcgagcgg aatgcaatgt
      721 cctcccagtc cgggtccggg atagaacgcc tgaaatccgt aaggtttggt tttggctgca
      781 tcaatgactt cccagaggtt gatgttcatt ttatgacaaa gcattgccag ttcgttcacc
      841 agaccaatat taatgttgcg ataggtattt tcaagtattt tttccatttc ggcgattgcc
      901 ggactcgaaa ctgtgtgaac atcgctatca agcacggcgc gatacatggc tgcaattact
      961 tccagtgctt cgtctccaat ggctcccaca actttgggtg tattttttgt tttgtaaatc
     1021 aaatttcccg gatcgacccg ctcaggcgaa aagccgagat agaaatcctc accgcatacc
     1081 aaccccgaac ctgcttcaag aattggtttg acgagttctt ccgtagtacc cggataggta
     1141 gtggattcaa gtaccaccat cgtacctttt ttcatataac gggcaatgga ttcggtagat
     1201 gatttcacgt aacttatatc cggctgctgg tacatatcca gcggcgtggg cacgcaaata
     1261 gccacaaaat caacatcgcg gataaaactg aaatcgttgg tggccgaaag cattcctttt
     1321 cccacaattt ctttcaaatc gtcgtccacc acatcgccaa tgtagttatg accggcattt
     1381 accatgtcca cttttgcttc ctgcacatcg aaaccgatgg ttttgaatcc ggctttggct
     1441 ttttcaacgg caagcggcaa tcctacatat ccgaggccta ccactcccac aacgatttcg
     1501 cgctgttcaa tttttttcag tacattttct tttatcatgc tctatatgct tgttgtattc
     1561 tgttaatcgg atattttcaa ctgacaaaaa tacaactttt ttttcagaaa cgaatcaggt
     1621 tattctggtg ttttgatttt gatggttaga aattgtattt ttggatattt aggccgaata
     1681 ctgaagtatg gatgaggcac ataactttaa taattcagcc taaaaaaagc acctgcaaat
     1741 gcaagcgctt tctgatatta ccatgtagat gatttatttt ccgtacatgt tctcgtagta
     1801 tttttcatat tcgccgctgg tcacgttgtc catccattcc tggttttcga gataccattt
     1861 cacggttttc tcaattcctt cctcaaactg gagcgagggt tcccacccga gttcgttttt
     1921 cagcttggta gaatctatgg cgtagcgcaa atcatgtcct gccctgtcgg tgacgtaggt
     1981 aatcaggccg agcgaatgtc cttcggggtt gccgagcagt ttatccaccg ttttaatcat
     2041 cacttttatc aaatcgatat ttttccactc gttgaatccg ccgatattat aggtatctcc
     2101 cggtttaccc cgatgaaaaa tcacgtcgat agcgcgtgca tgatccacca cataaagcca
     2161 gtcgcgcacg ttttcgcctt tgccgtaaac gggaagcggt ttttcgtgcc gtatattgtt
     2221 gataaatagc ggaataagtt tttcggggaa ctgatatgga ccgtagttat tcgagcaatt
     2281 ggagataatc acattcagac cataagtatc gtgaaatgcg cgtacaaagt ggtcggaaga
     2341 ggctttactg gccgaataag gcgaatgtgg atcgtaactg gtcgtttcgg taaacatgct
     2401 gccgtcaaat tccagcgctc cgtacacttc gtcggttgaa atatgataga aacggtgcgc
     2461 agcgtagtct tctttccaca tttccttagc agcctgaagc aaactcaggg ttcccattac
     2521 gttggtctgt gcaaatgtga acggatcttt gatgcttcgg tccacatgac tttcggcagc
     2581 cagatgtatt acatctgtaa cttcatacgt tttgaagatt tggagcatct gctcaaaatc
     2641 acaaatatca gctttcacaa accggtaatt cggagcattt tcgatgtctt tcagattagc
     2701 cagatttccg gcatacgtaa gtttatcaag gttgatgatg cggtaatccg gatactgatt
     2761 cacaaacaga cggacaacat ggttgcctat aaatccggca cctccggtga tgaggatatt
     2821 tttagcgttt tttttcattt cagtattttt gttaaattgg tttcgaataa tgatttttgg
     2881 gggtgaaatc cctgttttat ggtagcaaat agttcgtttt agattttcag gggtgaaacc
     2941 ccttttttat ggttgcaaat agttatgata tgaatgttta cctgactgta tgtgtttgcc
     3001 ataatattaa ccataaaaca ggtgcgatgc acctacgtat tcttttttat aatctttcct
     3061 accataaaat aggtgcgaag cacctgcata ttcttttttg aaatctttca taccataaaa
     3121 gaggtgcgat gcaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacctg
     3241 catattcttt tttgaaatct ttcataccat aaaagaggtg cgatgcacct atacattctt
     3301 ttttaaattc tttcctacca taaaataggt gccacgtacc tacatattct tttttataat
     3361 ctttcctacc ataaaagagg tgcgatgcac ctgcatatta tttttttgaa atctttttcc
     3421 taccataaag gaggtgcgat gcaccttgaa tgtaaattcc ttatcgagag cctgtaagat
     3481 tttttcaata agatttaagt catttctctc agcgaaacct tccagtacgg tatttttaac
     3541 ccaaacgttt cttttatttt tgttttgtct aaaacagaaa aattcggcct tttcactttg
     3601 cttggaaatt catcggagtg gcagggctga atgtcgcaca ggttgccgct cagattcctg
     3661 atttcgatgg caaaatcata ccaggagcat actccttcgt tggagaaatg ataaataccg
     3721 gcattgtcag cgtattttcc cgaatcaatt attttcagaa ttgcctgagc cagatcgcca
     3781 gcatacgtag gcgttcctac ctggtcgaaa accactttca gactatcctt ttcggctgtg
     3841 agtcgtcgaa ttgttttcac aaaattattc ccgaaagaag aatacagcca cgaagtgcgg
     3901 ataatcagat aatcgcaccc ggaatctgtc accgcttttt caccgtcaag ttttgtatgg
     3961 ccgtaaactc caagcggagc ggtggcatcg gtttcgagat atggtgtgtt cttgctcccg
     4021 tcgaacacgt aatcggtcga gatgtgaata agtatcagtt tgtattttgc acttacctct
     4081 gccaggtttt tcaccgccag gtgattgata ttatccgcca tttctgtatc gtcctcggct
     4141 ttatccacgt tggtgtatgc agcgcagtta acaatgatct cagctccgtg ttccgacacg
     4201 taattgtcaa cggcttcccg attggtaatg tccagttcgg ccacatcggt aaagagaaac
     4261 tcgtgattag aattcacgga cagcacacgt aattcacttc ccaattgccc gtttgctccg
     4321 gtaacaagaa tttttttact catataaatt gattgaatag tcaaacaaca catccgcctc
     4381 gctcaacaaa ggattattcc tgtccttttc cgaaagcaga atttctgctg ccggaatttt
     4441 ccagtcaata cctaatgctg catcgtccca ggctattccg gcttcggact gaggagcgta
     4501 ataattatca catttgtact gaaagacaac cgtttcactc aacactacaa atccatgtgc
     4561 aaatccacgc ggaacgaaca tctgacgttt gttctcgtca ttcagttcag cggccacgta
     4621 ctttccgaac gtgggcgaac ctttgcgaat gtcgaccgcc acatcgagca cgcttccctg
     4681 aaccacacga actaacttgg attgtgcgta aggagctttc tgaaaatgaa gcccccgcaa
     4741 tacgccaaac ctcgattttg attcattgtc ctgtacgaac tccgttttaa acactttttc
     4801 ttcgaactcc cgctgcgaaa acgattctaa aaaataaccc cgatgatcgc catacacatc
     4861 gggttccaga atcttcactt cagctatttc tgtatcaatt attttcataa atttatcagc
     4921 cccttacccc cgaaggggga aatagagagc acttttcgtt atttttgagg ccccttaatc
     4981 cctgaaggag aaatgagagc gcttttttca atgttttaaa aaatcaggaa atacgtttgt
     5041 ttaaaaccaa ctactttttt caatcaaatt tttcacgtag tttttgaatt tttctgaaaa
     5101 gatggcggtt aactgtactg tttttcatcg cagtattctt acgctcattc ttttggggag
     5161 atggaggggg cagaatttaa caaccccatc aaatactgtc cgtattcatt ttttatcatc
     5221 ccctgagcta tctgcctgag tcgttcgtcg gtaatccatc cgttgcgcca tgcaatttcc
     5281 tcgagacacg agattttcag gccctgccgt ttttcgagca cttcgatgaa ggttgatgct
     5341 tccgccagcg aatcgaatgt accggtatcc agccaggcaa atccgcgtcc gaaaagttga
     5401 accttcagct gctcgtcttt cagaaattcc tgattgacag tcgttatttc cagttctccc
     5461 cgtgcggatg gtttgatgtt ttttgcaaca tccaccacct tgttcggata gaaatagagt
     5521 cctaccacag cataattcga ttttggttcc ttcggttttt cttcgatgct gattacttta
     5581 ccgtcattat cgaattctgc cactccgtat cgttccgggt cgttcaccca atatccgaaa
     5641 acggttgcca ggttgttttc agctgcgtct tttgcagcat ttcggagcat ggttgggaaa
     5701 ccgtgtccgt agaaaatgtt atctcccagc accaggcatg ctgcatcgtc acctataaat
     5761 ttttcgccaa tgataaaagc ctgtgccagg ccatcgggac ttggttgctc ggcatactca
     5821 aaattcaccc cgtaatccga gccatcgccg agtaaacgtc tgaaaccggg taaatcggca
     5881 ggtgtggaaa tgatcaggat atccctgatt ccggccagca taagtgccga aatgggataa
     5941 tataccatcg gcttatcgaa aatcggaatc aattgtttgg aaataccttt cgtgatgggg
     6001 taaagtcggg ttccggagcc tccggcaagt actattcctt tcatgagtaa cttttattta
     6061 ttgtaaaaaa aaataattat aaccaatact aatcaaagat aataggtaat acccaccgaa
     6121 agtgcaggca ttatatccaa tttactcccc atttcaaaac cgttgtagtt gtcatcaaca
     6181 atattaagtt caaaaatggc tttcaaatcc atgtattcat tcaatatgaa attagtctgt
     6241 gctcctaacc tgcctatcat actgaacaaa ccggcaccat ttcgggtcat tctgtttctg
     6301 aaatgaaagc ctgtgccggc aaaagccaga aaatcgaaag agcgggcaag gttctgccct
     6361 tcgaggtaat tgctgatatt catcatcaca tcacctgtta catatttgga ggcaaatgaa
     6421 atcacttctt tgtagtaaag cgtggcttcg ggccagtgat tttgggtgaa acctgcaaaa
     6481 cctcttattc cccataccgg agaatagttg tagcctattt cagctctgaa catcgaaccg
     6541 gagtttctgc ctaatgaata ccggctgtcc ggattatttg aaaaattgtc gatcctctcg
     6601 cctgccagcc agctggtgcc cagattgcca cctacatacc atcccgggta aaaaccatca
     6661 tttccggccg tttgagccga caccagcagt gtggaaaata aaataccgac aataaataca
     6721 atttttctca tcgcaaacag gtttattttt actttttcgt ttgttttttc aggttattaa
     6781 tacattccgg atcaccaaaa aacaatcagc actctccgat acagactgct gccatatttt
     6841 tttttcagat tgaacaaaaa aagtaaagaa attgattttt ttgattgaaa tacaaaagta
     6901 agcatttttc ttctactctg aaaaacagaa atgaaaatag gaagaaactc tccggctgat
     6961 actttttttt caaggaactg aaattcgtta caagatggaa aaatttattt ttatgatgaa
     7021 aaaagactat ctttacctgc taaaaaacat ttatattata cggatgtttt agttacaaaa
     7081 caggaaaacg gcttattgca atgtcaattt taccggcgaa tttttgaaaa aaataaataa
     7141 tttctatttc aacgtgaaaa aagatgaaca caaaagactt cggatagata acacaaaaaa
     7201 gttctacaac gttgttttta ttgatctggg cgaactggcc attttcactg cgagagtgat
     7261 ccggcaattc gtttcgcctc cctatgagca tcgggaacta atcaggcagg cttacgtcat
     7321 cgggaacaag tctgtggcaa tggtggcact aaccggtctc atcatgggtt tggtactcac
     7381 catgcaaact cagcctacgc tggtacagtt tggggttcag tcgctcatac ccgggatggt
     7441 agccgtttcc atgtttcggg aggtaggtcc cgttgtcacc ggattgattt gcgccggcaa
     7501 aataagctcg ggtatcggag ccgaaatcgg ggctatgaaa gtgaccgagc aaatagatgc
     7561 catggaagta tccggcacca aaccgctgag ttttgtagtg gctacgcgtg tgctcgcaac
     7621 aaccgttatg gtaccgttac tggtgttttt ttctgacgcg ttctccctca ttggttcgta
     7681 cctgggagtg aatttgtttg aatccatgag tttcgaactc tttatgaaaa atgcatttga
     7741 catgcttggt ttcatggatg taattccggc aaccatcaaa tcggttgtat tcggattctt
     7801 catcgggcta ataggagctt acaagggtta tcaggccgga ttgggaaccg aatcggtggg
     7861 acaggctgcc aattcggcgg tagtaaacgc atcactttcc attttcatca tcgacctggt
     7921 tgctgtactg attactaatc ttttaacagg caattgaaat gatagaagtt aaagatttgt
     7981 ataaagcctt tggagaaaat gtaatactaa atggtgtcgg tttccacctc ttgaagggtg
     8041 agaatctggg cattctcgga aaatcaggat caggaaaatc ggtgctcacc aagtgcattg
     8101 tccgcctgat tgaaccggac ttcggcgaaa ttaaagttct tggaaaggac attctgaaaa
     8161 tgtcgctgaa tgagttgaac gaaatccgga aagacattgg ttatcttttt cagggtggcg
     8221 ccttgtatga ctccatgaat gtacgcgaaa acctggagtt cccgatccga cgcactcaga
     8281 tggcgagcaa caaacacgaa gtgaacgaac gggtggaaca ggcgctgagg agtgtggggc
     8341 tgctggatgc aataaacaaa atgcctgcag aactctccgg aggcatgaaa aaacgaatcg
     8401 gcctggcaag agcgctgatt ctaaggccga aagtaatact gtacgacgaa cccaccaccg
     8461 ggctcgactc ggtgacctcg ggcgaaatca gcgaattgat tctgaaagta caggaagaat
     8521 acaatgcatc gtccatcatc attacccacg acatgaaatg tgcaaaaatt gctaccgata
     8581 aaataaaaat cctggaaaat ggcacatttc tgacggaagg aacttacgat gaattaagca
     8641 aaagcaccga cagacaaatc agagcatttt ttatgtaaaa aaaagaactg cagttgtatc
     8701 aactcttttt ttaatccgaa aaaattagaa taaatgaaaa atcaaacaaa taaagcactg
     8761 cgggtaggat tatttgtatt taccgcgctg gccattttca tcaccatcat ttacctgatc
     8821 gggagtaaag acaatttgtt caaaaccaaa accacaatta ccacttcgtt caacgacata
     8881 cgaggcgtgg tggtaggcaa caacgtccgt ttttcgggga taaatgtagg aaaagtcagc
     8941 gatatcaacg tcgtttcgga caaggaagtt gtgcttgaac tgtccatagt gaaagaatat
     9001 gcccgtttta tttacaaaga tgcacttgta gagattaatc aggacgggct gatggggagc
     9061 aaattaatta acatcacctc cggtcattca tcagccggaa tgatagagga aggcactcat
     9121 ctgaaaggga aagaaggtat tgacattgaa aatatgcttt acgaagtaca cgatatactc
     9181 gttgaggcgg gtgatgccgt tgccagtctg aaatcaatag ccggaaaaat cgattcagga
     9241 gagggtgacc tggcaaaact ggtgaatgac gacgtactct ctacagaact gaccgccacc
     9301 acccggcagc tgaatactac actggcaacg gtggaacaaa ttaccaaaaa catgacaacg
     9361 ggcgaaggtg acatagcacg actgatcaac agcaacgagc taacctcgca ggcacatcag
     9421 ttattgggca atctgaacga agctgtggag aaaacgcatc gggttgctga aaatctcgaa
     9481 accacttccc gtcagataaa ctacggcgag ggtgctgtaa atcttttgct gaacaataaa
     9541 aacaccgctc aaaacatcga tacaaccatt ctgaagttac agaacagcct gaacgaattt
     9601 gataaaacgg ccagagccgt acagaacagc tggctgattc agctattcaa cagaaaaaaa
     9661 ctcaaagata aaaaaaccgc taaagactcc ataaaataat gaaacaaccg aagttcatca
     9721 cttcggttgt ttcattatta aaaccactcc atcattaaat ggcttctgtt ttacgactca
     9781 gccattttct ttccttttcg gtaaggttcg ggcaaagcgt tttgcaaaca cgaagatggt
     9841 aatcgttgag ccattttctt tcgttatctg taagcatatc tttttccacc agacgggtat
     9901 cgatatagca aagtgtgagc gtttcgaact tcaggaactg accgaattcg gttttttctg
     9961 ccggaatcac ctgtaccagg ttttcgattc gtattccata ttctccggtt ctgtaaagtc
    10021 cgggctcgtt tgaaatcatc atgcctggct gcagtgtaac cggattttcg tccatccgga
    10081 tgttttgcgg accctcgtgc acattcagaa aatgtccgat tccgtgaccc gtaccgtgtc
    10141 cgtagttaat tccttcgtcc cacatggctt tccgggccag aatatccagc tgcgagccgc
    10201 gtgtgttcac cgggaatttt gccaatgcca actgaatatg ccctttcagt accatggtga
    10261 aatctctttt ttcctttttg gaaggtttac caagtgcaat ggttcgggta atatcggttg
    10321 tgccgtccag gtattgtccg ccagaatcaa gcagcaggat tcctacattt tttatcaccg
    10381 catcgctttc gggagtagct ttgtagtgca ctatagctcc atgacctgca tatccggcaa
    10441 tagttccgaa actttcgccc ctgaaacctt cctggtcttc gcgaaaggag cgaagtttat
    10501 cagaaatgga taattctgtg agtttttcat ttttcagact tttctcaaac cacatgaaga
    10561 aacgtgtcaa agctactccg tctttaatca tcgcatttcg aacgccacgc atttctgttt
    10621 tgtttttcac acttttcagt ctgaaaacag gcgacatggc ttttctctta atgcattctg
    10681 caggaatagc ttcaaaaagt gattgattca gtttcccagc atccaccaac acagcttttg
    10741 atttcggaat tttttgtaat tcttcgtgaa tattgaagta atcctttact atgatcccgt
    10801 ttttgtcgag gtatttttta gtttctgtcg ttattttttc ggaagagata aataatgttg
    10861 ccgatttttc gtcgatcaat gcgtaagtaa tcaccaccgg attgtagtcc acatcgttac
    10921 cgcggatatt aaacagccag gcaatatcat ccaaagctga cattacaaaa acatcggcat
    10981 aaagtttttt catttcgtgc cgaagttcgt cgagcttctc tcccaccgat tttccggtat
    11041 attttttatc aaaaatgaaa aacggatttt tcggcaattc aggtcgatca atccatacat
    11101 ctttcattaa atcaatggat accaattcaa tgtcactcag tgcaagttcc gttttcatat
    11161 tctgatagcc gtttaccgaa aacatttcgg cactcacggc tactttttcg cctttacgta
    11221 gctctgtcga gagccacgga ataatttcca gcgtttcagg cagtccctgt ttcatcaatt
    11281 gaatttcagt accttccagc tgctgcgcac cctgaagaaa atagcgtgaa tctgtccaga
    11341 gtccggcttt ctggtgagtc aaagccaccg tgccggccga tccgtcaaat ccgcttatcc
    11401 attcccgctc tttccagaaa tcggcgatgt attcactggc gtgtggatcg gttcccggga
    11461 ttatacaagc cgctaccccg gcttttttca ttgcttcgcg caatgcacta attcgtccgt
    11521 taatttgtgt catatatttg attgttagat tgtttgggtg tttgggtgtt ttgttgtata
    11581 gttgtatagt tgtattgtcg ttttgttgta ttgttgtatt gttgtatagt tgtattgtcg
    11641 ttttgttgtt ttgttatagt ttgaagttgg aacacacaaa ttataaagca gaattaaaca
    11701 attcgtataa ttcgtactaa ttcgtgaaat tcgtcctaat tcgtgaaatt tgtattttaa
    11761 acaaagatat aaaattaaaa ctttatgctt tcgaccaatt aaacttgatt aacacaaaaa
    11821 caaatcacaa aatcattttg tttataataa aaccctcaaa aaacatcgtc aacatggatt
    11881 tgatcaatca accgctgaga attaaaaaca cagtacttaa aaaccgtatc gggatgtcgc
    11941 ccatgtgtat gtattctgct gccgacggat ttgcaaccga ctggcatctg gcacattacg
    12001 gtgcacgggc tacaggcggt acggcactta tcattcagga ggcaacggca gtgacccccg
    12061 aaggaagaat tacaccttat gatctgggta tctggaaaga tgaacacatc gagcaactga
    12121 agaaaattac cggttttatt tcttcacaag gtgccgttcc ggctattcag ctggcacatg
    12181 ccgggcgtaa agcttcgcac gacaaacctg cgaatggcgg taaatttctt tctcccgaac
    12241 acggaggctg gcaaacagtg gctccttcgg cagttccatt ttcacccgaa acaaatgtgc
    12301 ccacagcatt gaccgaatat ggcatttacc agattattga attatttgca aaagccgccc
    12361 ggagagccgt taaagccgga tacagaatta ttgaaattca tgcagcccac ggttatttga
    12421 ttcaggaatt tatgtcgccg ctgagcaacc acagaaacga tgaatacggt gggagtttcg
    12481 aaaatcgaat ccggctgctt ttgcaagtgg tggaagccgt aaaacgtgtg attccggatg
    12541 aaatgccctt gttcgtgcgg gtgagcgctt cggaatggac cgaaggcgga tggacgattg
    12601 atgaaaccgt gaaactgact gaaattctaa tggagaaagg agtcgatctc atggattgtt
    12661 catcgggcgg caatattgcc gatgcaaaaa tggcacccgg accgctctat caggttccat
    12721 ttgctgaagc tgtacataat acaggcataa aaacagcagc tgtaggcctt atcaccacac
    12781 cgcaacaagt caattcgatc cttgaaaaag gtcaggcaga cctggtactt ctcggccggg
    12841 aattactccg gaatccgtat tttgctttgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn ngttattaaa attgacatat tttggatact actaaaaata ttcatttacc
    13021 ggataccact gaaaatgtgg atgttatcgg agcaagagtt cataatctga aaaatatcga
    13081 cgtttccatt ccgcgcgatt cactcaccgt aatcaccgga ttaagtggca gcggaaaatc
    13141 atcgctggcg tttgatacca tttttgcaga aggtcagcgt cgctatatcg aaactttttc
    13201 agcctatgcc cgcagttttc tgggtaatat ggaacgaccc gatgtggata aaattacagg
    13261 attgagtcct gttatttcta tcgaacaaaa aacgaccaac aaaaatccac gctccaccgt
    13321 gggtaccacc accgaaattt acgactttct tcgtctgcta tttgcacgtg cgggtgttgc
    13381 ctattcgtat ctctcgggcg aaaaaatggt aaaatatacc gacgagcaga ttgtgaattt
    13441 gattttacaa gagtacgaag gcaagaaaat atacctcctt gcacctgtag tacgtggccg
    13501 aaaaggacat taccgcgaat tattcgaaaa catccgaaaa aaaggttaca tccacgttcg
    13561 tgtggatggc gagctgtgtg aaatcactca caacatgaaa gtggatcgct acaaaatcca
    13621 cgacattgaa atcgttatcg accgcctgat tattacggcc aaagaccgga agcgtctgct
    13681 cgattcaatg caaaaagcga tgcaacaagg taatggcgta attatgatag cccccccact
    13741 ccccgaaaag gcctcctcta actcacccaa agggggagaa aatatccctc attcggcgga
    13801 gttagacggg gctcgttact tcagccgaaa actgatgtgc ccttccaccg gaatttcgta
    13861 cgatgagccg gcacctcatc atttttcgtt caattcgccc cacggctact gcccgcggtg
    13921 caaaggtctt ggatttgtca acgaaattga tatggaaaaa atcattcctg ataaatctct
    13981 gaacatccac aacggtggaa tcgcaccgct cggaaagcat aaaaacagca tgattttctg
    14041 gcaaatagaa gccattctgg aaaaatacga atgcacgttg aaaactccaa ttgttgacat
    14101 accggacgat gcgatagatg aaattatgaa cggaacagac gaacggctga acatcaaaaa
    14161 ccagtcgctc ggaaattcca attactttct gaattacgat ggaattctga aatacatcga
    14221 aatgcagcag gagaataccg cttcggcaac ggagcaaaaa tgggcaaatc agtattcaaa
    14281 aacaagcaaa tgccccgaat gtaacgggca acggctgcga aaagaatcgc ttttcttccg
    14341 gattcacgat aaaaacattg ccgaactggc agaaatggac atgctggaat tgaatgagtg
    14401 gttgaaaaac gtcgagcagt tcatgtctga caagcaaaaa accatcgctt cagagattct
    14461 gaaagaatta aaaagccgtt tgcaattttt gctggacgtc ggtctgaatt acctggccat
    14521 gaaccggcct tctctttcgc tttcgggggg cgaaagtcag cgcatccggc tggccactca
    14581 aataggatcg caactggtca atgtacttta tattctggat gaaccgagta tcggactcca
    14641 tcagcgcgac aacgaacggc tgataacgtc gctgaaaaaa ctccgcgaca ccggaaattc
    14701 cgtaattgta gtggaacacg ataaagacat gatgctcgca gcagattaca ttgtggacct
    14761 gggaccccgc gcagggcgat tgggtggcga acttgttttt gcgggaacac ccgatgagat
    14821 attactttcg gacacattga ctgccgacta cctgaccggt aaaaagaaaa ttgaaattcc
    14881 ggaatccatc cgaaaaggaa atggtaaatt ccttacaatt aaaggcgcca gaggcaataa
    14941 cctgaaaaat gtggatgtaa cttttccgtt gggaaaaatg atttgtgtta caggtgtatc
    15001 gggcagcggc aaatcgtcac ttatcaacga aacgctgcaa ccgattttga gtcagcattt
    15061 ctaccgttca ctgaaagatc cgctggatta cgatacggtg gaaggcatcg aaaaccttga
    15121 caaggtggtt caggtggatc aatcacccat cggacgtact ccccgctcca atccggctac
    15181 atacaccgga gttttcagtg acatacgcaa cctttttgct tctttgcccg aggcaaaaat
    15241 cagaggatac aaaaccggac gattctcgtt caatactgcc ggcggaagat gcgaaacctg
    15301 tggtggaaac ggatacaaaa ccattgaaat gaattttctg ccggatgtgt tcgttccgtg
    15361 tgaaacgtgc aacggtaaac gttacaaccg cgaaacgctc gaagttcgct tcaagggaaa
    15421 atccattgcc gatgtactca atatgactat caatcaggca gttgaattct ttgaaaacca
    15481 gcaatccata ctaaataaaa ttaaaaccct tcaggatgtt ggactgggtt atgtgaagct
    15541 cggacaaagc agcacaacgc tttcgggagg cgaaagtcaa cgcgtgaaac tggctaccga
    15601 actttcaaaa aaagacaccg gcaaaacgct ctacattctc gacgagccaa ctaccggatt
    15661 acactttgaa gatatccgtg tgttgctgaa agtgctcaac cggctggctg acaaaggaaa
    15721 taccgtaatc atcatcgaac acaacctgga tgtgataaaa gtagccgact acatcatcga
    15781 tatcgggccg gaaggcggca acagaggtgg ttacatcatg tgcgcgggta ctccgcagga
    15841 agtagccagc cacgcgcaca gcctgacagg aaaattcctt aaaaaagaat ttcaacagta
    15901 acaaatacca tcggatctga ttgtcgatta tacggttttc aggatgaggc aaattccatc
    15961 agttgataat tggaatttaa ttcttaactt tgtaaaaatc aattttctca actatgaatt
    16021 ccaccataaa attaatcatt tacgctatca gcactttcct tgtcttcctt ttactaacat
    16081 ggattttaag actaatggca ggaaaacttc ccattgaaaa tggcatactg ggcgtattca
    16141 aaaattcgga tctgttgctc ggcctcgttg ttgccgtagc tgtcaccttc tcgcatatcc
    16201 agaaaagaaa actcaagtaa cactatggaa tggaaaagga tttgacaaaa atatcggatt
    16261 acatcattcg cttcttgctg ggaaatgaaa atgccggatt tgcaaaacac atcggctatt
    16321 attccgacaa ggatgattat tccggctgca aactggtaat ccggcgctca aatttctttg
    16381 atccggatat ttacggaacg acacaatctt atcctgcatt acctttgtcg ctttggaaag
    16441 atattccgct gctttttggc agtcccgaaa caaaaaaatc gggcaacacc cttattttac
    16501 atgccgatat cattgcaagt actttttttc tgataagccg ctacgaagaa gtcatcctca
    16561 gggatgaacg cgaccaacac ggacgctttc cgggtaaaaa atcgctggca ttcaaagccg
    16621 gatttttgca acgacctctt gtggacgagt acggcgcaat gctgagaaaa ctgctccgcg
    16681 aaaccggata caacatcaca gaaccgtctg aaaaattccg taaaatctac ctgactcacg
    16741 atgtggacca gctggctcat taccgcaatt tcagaggaat ggccggtgcc gtttcacgct
    16801 ttttcaaaaa tccatatcaa accggtaaag ctgtaaagtc ttatttcgga ggtattggga
    16861 atgacccctg gtacacattc ccctggtttt ttgatctggc agaagaattg cgaaaagaga
    16921 aaccggaatc aaactgtgaa agcattgtct ttttcaaaac gggaggcggc gacctgatga
    16981 ccgataaacc tcttcacgat gtaagagata aagatttcaa ccggttattt tcactttgca
    17041 agcaattcgg ggtaaaaatc ggacttcatc cctcctatca ggccggaatg aatccgaatt
    17101 atattcaaaa agaaaaaaag atactcgacg aaaccactga ccaacagaca acttactccc
    17161 gcaaccactt tctaagcaat cgggaaccgg agcacatgca ggcattgatt gaagcaggta
    17221 ttaccgatga tttcaccatg gcttatgccg atatcgcagg tttcaggttg ggtacatcaa
    17281 gaccggtaaa atggattaat ccgctcaccg gggagatcac cgatcttacg ctgcatccgc
    17341 tcatattgat ggacagcact ctcagcgacg agcgatacat gaaactcaga gccgatgaag
    17401 cattttcata ttcaaaaaaa atgatagatc tcgttaaaaa acacaacgga gatttagtgt
    17461 tgctttggca caatactacg gtggaaaaac acaacggaca gtatcacagg gatttgtacc
    17521 gttggatgat aaattatctg aaaatgaatc tgtaacaatg tcaaaaaaag ccatagtttg
    17581 tgtaaccaac gacctgacaa ccgatcagcg tgtgagtaaa acctgcacta cgcttttaaa
    17641 atgcggttat caggttatag aaaccggacg attgctgccc gacagcctgg agtttgcacc
    17701 ttcttatacc atccgccgaa agaaattgtg gttcaacacc ggtgcacttt tttatgccga
    17761 atacaacatc aggctgtttt tttacctcct ttttgctcgt gttgacctca ttttcagcaa
    17821 cgacctggat acactgccgg cagcattcgt cgtatcagaa atccgacgaa aaaaactcat
    17881 ttacgattct cacgagtatt ttaccgaaat gcctgaattg gttggtagaa agaagataca
    17941 ggcaacctgg aaatttttcg agcgtgccat ttttccgaaa ctcaaacaca tcatcaccgt
    18001 aaatcattcg atagcagatc tttatgccaa agagtatcaa aaagaaattc atgtggtgag
    18061 aaatattcca cccacctaca atcctgatgt gattaaaagc cgtaaagaac tcgacctgcc
    18121 ggaagacaag aaaatcctga ttttacaggg aactggcatc aatatcgacc gtggcgccga
    18181 agaagccgta atggcgatga aatatattga aaatgccgtt ttacttattg tgggcagggg
    18241 cgatgttatt cccgcgttac gacacatagt gcaggaagaa aaactggaac acaaaatcat
    18301 tttcaaaccg aaaatgcctt ttaccgaact caggcaacac accatgaatg ccgatctggg
    18361 tcttgtcatt gacaaagaca ccaacctgaa ttaccgtttt tcgctgccca acaaattgtt
    18421 cgattatatt cattcagaca taccggtgct cgcatctgag ctgcccgaaa taaaaaatat
    18481 aatcgacaag tacgatatcg gatattatat tccaaatcac tcgccacaag caattgctga
    18541 aactgtaaac cggatttttg aaaacaaatc ccgttaccag actgttaaac acaatactca
    18601 gaaagccaaa acggaattat cgtgggaaaa agaagaactc aatctgattg aggtgattaa
    18661 caacgtcaat ctctgatttc gaatccgaaa aaaaaatctg aaatgctgtc aattcttata
    18721 ccaacataca actacaacat tacacatctg gtgaacgaac ttcaccggca ggcaatgaat
    18781 acatatgttg attttgaaat tttggtaatg gaggacggtt caacacagtt tgtgcatgaa
    18841 aacaaagcag tagctgattt aaagtattgc aaatacacca ttctcaccaa aaatatcgga
    18901 cgttcggcca tccgtaaccg gttagccgac gatgccagat tcaatcacct gctttttctg
    18961 gattgcgaca gcgaagtaat catgcctcat tttatcgaaa aatacattgc gttttgcaaa
    19021 gaaaactcgg tagtggtagg aggaacagca tacgataccg gcaataaaaa tccgaacttc
    19081 agtttacgat tgaaatacgg gcgtctccgg gaagccaaat cggctctgga acgcgaaaag
    19141 gatggactat attcacattt ctcggctttc aattttctga tatcaaaaag cacattcaac
    19201 caaatccgtt tcgatgaaga cattcagggc tacgggcacg aagacacatt attcggtcat
    19261 aaaatagcag aactcaaatg cattatgcac catattgaca atccgctgct ccaccggggg
    19321 ctggacgaaa acgatatttt catccgaaaa accgaagaaa gtaccagaaa cctctatata
    19381 ctgtacctta caggaaaata tccatccctc gaaaagcagt cgaaattatt gtttaaattc
    19441 aatcagttca agaagaaaaa ttttgtctgt gttctggcag cactcttcac agcacttcaa
    19501 cctgttttaa tgagcaatct gacaagctcc gctccctctc tcagacttta cgatttatac
    19561 aaactacttt ttcttgccaa aatttcacgc tcgaaatgac aacttgtcag tttcttgcgc
    19621 tctcaacacc cggattgttc tttttttatg ccaaaatttc atttacattc cgttggcata
    19681 catttcgcta actatcacat gttttgaaac aagtaaacat atcaataaac tctaaaaata
    19741 ttaatcttat gaacattaaa cccttagcag atcgcgtgct ggtaaaaccc gcaccggcag
    19801 aagaaaaaac aataagcgga attatcatcc ccgacagcgc aaaagaaaaa ccgttgaaag
    19861 gcgaaatcct ggcagtagga aatggcacca aagacgaaga aatgattgta aaagttggaa
    19921 atactgtact ctacggtaaa tatgccggaa ccgaactcga atgggaaggc gaaaaatacc
    19981 tgatcatgcg tcagaatgat attttggcaa ttatttaaaa attagaagtc agaaattaga
    20041 aatcagaatt ttgtttgcct ttggcagatt agatatatga gctaattaaa tgacgcaatc
    20101 gtaaatcgta aattattaaa tcgtaaatat aaaaatggca aaagaaattt tattcaatat
    20161 cgatgcccgt gatcaactga aaaaaggagt tgacgaactg gcaaacgcag taaaagtaac
    20221 tctcggacct aaaggccgca acgtgatact tgaaaagaaa tttggtgctc ctcatatcac
    20281 caaagacggt gtaaccgtgg caaaagaaat tgaattggaa gatgcatacc agaatatggg
    20341 tgcacagttg gtaaaagaag tggcttcaaa aaccggagat gacgccggag atggtacaac
    20401 aacggctact gtattagctc aatccattgt aaatgttgga cttaaaaacg tgactgccgg
    20461 tgcaaatcca atggatctga aacgcggtat agacaaagct gtggaaagcg ttgtaaaaaa
    20521 catagcttca caagccagaa cagtaggtga caactacgac aaaatagaac aggtagctac
    20581 catatcggcc aacaacgact cacgcattgg caaactcata gccgatgcca tgcgcaaagt
    20641 ttcaaaagac ggagtaatca ccatcgaaga agcaaaagga accgaaacaa ccatcgaagt
    20701 agtggaaggt atgcaatttg accgcggata catctctccg tatttcgtaa ccaacaccga
    20761 acaaatggaa gtggaatttg aaaaaccaca aattcttatt tacgacaaaa aaatctccaa
    20821 cctgaaagaa ttgcttccaa ttctggaacc agcagttcaa tccggtcgtc cgattctgat
    20881 catcgccgaa gatgttgaca gtgaagcact tactacactg gttgtaaacc gtctgcgtgg
    20941 ttcgttgaaa atttgtgccg taaaagctcc gggctttggc gaccgccgga aagaaatgct
    21001 cgaagacatc gccattctga caggtggaac cgttatttcg gaagaaaaag gtattaaact
    21061 tgaaaatgca accctcgaaa tgctgggtac agccgaaaaa atcacggttg acaaagacaa
    21121 caccactatc gtaaacggtg ccggcgaaaa agacgccatt caaacccgca tcggccagat
    21181 taaatcgcag attgcatcca caacttccga ttacgacaag gaaaagctgc aggaacgcct
    21241 ggcaaaactt tcgggtggcg tagctgtatt gtatgtagga gcagcaagcg aagtggaaat
    21301 gaaggaaatg aaagaccgcg tggacgatgc actacatgct acccgcgcag ctatcgaaga
    21361 aggcatcgtt cctggtggag gtgtggctta tattcgttca atagatacac ttgacaacat
    21421 tactcccgac aatgaagacg aacgtacagg tatcgaaata gtaaaacgcg ccattgaaga
    21481 acctcttcgt cagatcgttg ccaacgcagg taaagaaggt gccgttgttg ctcaaaaagt
    21541 acgtgaaggt aaagcagatt acggatacaa cgcccgcact gacaaatatg aaaacttctt
    21601 cgaagcaggc gtggtagatc ccgctaaagt ttgccgcata gctcttgaga atgcagcttc
    21661 catagcaggc atgttcctga ctaccgaatg tgtgattgtt gagaaaaaag aagataatcc
    21721 ggctcctcaa atgcctatgg gcggcggaat gggcggaatg atgtaaaaaa gccccctaaa
    21781 tccccctcag ggggacttca gaacaaagca taatgacaaa ctccccgaat cgaatatgat
    21841 tcggggagtt ttttgtttgt ttagtaaaac ttttcactaa aataatgaga attattcctg
    21901 ttcttccctt ttttttcttt actttgcaat cataaaactt aaaaaataac ggcgatgaaa
    21961 attggaattc tatcttcggg cggcgactgt cccggtatca atgctacgat acgtggcgtt
    22021 ggaaaaacag ccatcagcaa atacggaatg gaagtaatcg gcatccagaa tggcttctca
    22081 ggattgttat acaaggatat aattcctctt actgaaggtt cattatcagg tatcctgact
    22141 ttaggaggca ccatactggg taccgctcgc gaaaaagagt tcagaaaaat tctgaaatcg
    22201 cccgacaaga aagatcagga agtaatcaaa aaaagctaca aggaactggg tttggattgt
    22261 ctggtatgta tcggtggaaa cggcacacaa aaaaccacct ggcaactctc cgagcttgga
    22321 ctgaatgtaa tcggcatccc caaaaccatc gacaacgatg ttttcggaac cgatgtgacc
    22381 ttcggtttta attcggccgt ggacatagct accgatgcta ttgacaggct ccattctaca
    22441 gcaagttcac atcagcgggt gatggtcatc gagctgatgg gacatcatgc cggatggata
    22501 acactctacg caggtatggc cggaggagcc gatgtgatac ttttacccga acttggatat
    22561 gacctgaata cggtgacaga agtgctcaac agacgcaaaa aaagcggaaa agcttattca
    22621 atcgtagctg tggcagaggg aattgtagta gaaggaggaa ccgacggaca tcatccggct
    22681 acgtattttg cccgaaaaat tgaagaactc accggcatcg aagctcgtga aaccgtgttg
    22741 ggttacattc agcgtggcgg atcgccttcg ccctacgacc ggaatctggg cactttgctt
    22801 ggcggacatg ccgcaaaact aatcaacgat gaaaaattcg gacgaatggt ttctgtgaag
    22861 ggcattgacg taatcacaga cgttccgctc aaagatgtgg caggaaaact tcgcctggtt
    22921 actcccgatc acatcatggt aaaacaaggc aaacgtatgg gtatttcttt cgggaaatta
    22981 ggtttataaa aaattcattt tatcaaaaca aaaaccgcaa ctctttttca gaattgcggt
    23041 ttttgttttt attactacag ggattatttt acagctttag ctttattttc ggtgtgattt
    23101 acgcttttta cgatagcgct gaatgcttcc ggatgattca tggctaaatc ggccagtact
    23161 ttacggttca tttcgatacc ggctttgtgc agagcaccca tgaggcgtga atatgacata
    23221 ccttccaggc gtgcagcagc gttgatgcgc tgaatccaca aagcgcggaa attgcgtttt
    23281 ttgtttttac ggtcgcggta agcatattgc aaaccttttt cccatgtgtt tttggctacg
    23341 gtccatacat tacgacgacc tccaaaataa cctctggtga ggcttagaat tctttttctt
    23401 cttttacgtg aagctacgtg atttactgat cttggcataa ttaaatgttt ttttgaaagt
    23461 tagcgctgct ttcagcagtc tttttagcta atcattcggt ttaacttgta agaaattttg
    23521 ttttaaaaag tgttgattct gactgcttaa acgcgaagca ttgcttttac gtttttaaca
    23581 tccacgctgt ccaccagacc agtctgggtc agattacgtt tttgttttgt ggttttttta
    23641 gtcaaaatgt gacttttaaa agcatgtttt cttttgattt ttcctgttcc ggtaagagcg
    23701 aatctttttt tggcaccgga gttagttttc atttttggca ttttatcctt ttttatttat
    23761 tagtgagatt tcgttattta gttttttttg gcgaaaacat gataatcatc cttttaccct
    23821 ccaactgagg aagctgatca actttggcat attcttccag atcgttggca aatcggagta
    23881 aaagtacttc accctgctcc ttgaacagaa tagaacgacc tttgaaaaac acatacgctt
    23941 tcactttatc gccttcctga aggaacttga tagcatgttt caacttgaag ttgtaatcat
    24001 ggtcgtcagt ctgaggcccg aaacgaattt cttttacatc tattttggtt gacttggcct
    24061 tgttctcctt ttcctttttc ttaagctggt acagaaactt ttgataatca acaattttac
    24121 aaacaggcgg tgccgcattc ggcgcaattt caaccaggtc caattcgaga tcttcagcta
    24181 tttgcatggc acgctctatg gtatagatgc cgggctcaac attttcacct actaacctga
    24241 cctctttaat gcctttaatc ttttcattaa ttctgttagg catttctttc tgttgacctt
    24301 gtctgggtct cataaatggt tgtttagcta ttgcttgttt cctcctaatt ttaagttatt
    24361 tatatgattt tctgtacact tttttgagcg tgcaaatata atgctttttt ttagatttac
    24421 aaaatatcat ggtaacgaat ttgagaaaat cctggttgag catatcttaa atacgacatt
    24481 catatgctgg gatctggcag taaactttcc tggcttaata aatcaacaca ctgattaaca
    24541 aagttttaat cagatgattt gtgtgtttca tatttattga gtaattttgc attctgaatt
    24601 tttgcagaaa gctgcaaatt aaaaaaccaa attatgaaga aactgatatt attgtttgtt
    24661 gtactttcaa tgatgttttc atcatcactg tacagccagc aaagagtgaa agcctcatat
    24721 tatgcagaca aatttcacgg aagaaaaact gccagcggaa agctttacaa ccgcgacagt
    24781 ttgacatgtg cacataaaac ctatcctttc gggaccatac tcaaagtaaa aaatccgcgt
    24841 aatgataagg aaatttatgt taaagtaact gaccgcggtc cattcgttaa agggcggtcc
    24901 attgatttat catacgctgc tgctaaaaaa attggtgtta tcggccatgg aatctcaatt
    24961 gttgatatca ctaaaacaaa tctgacacaa atccccaagc cgggagaaag aaagatgatt
    25021 ccggattcac ttaatcttga aaacattgaa atcaggagtg actccatcat tattcaaccc
    25081 gacaacacga aagtatcgaa cgacagcata aagctggctc tgaaaatcaa catcccaatt
    25141 aaaacaaaaa cagcctgatt atcaggctgt ttttgtttat cattgataaa gcagaaatta
    25201 aattattcct ctatttcatc atcatcacct tcttcggtat caaatacaga gagttgcctg
    25261 ttgaagattt cgtccagtga cagttgaaaa gtttctgtat gctgcactcc gtcaatggtg
    25321 gtaagatttt caagaattat ctgtagcaga tgccggttat cttttgcgta gattttgata
    25381 aacatggagt atttacccgt agcatagtga cattcaacaa tttcaggaac ttttttcagt
    25441 tcggctacca ctgattgaaa ttttttgatg tctgaaagca caataccaat ataggcacac
    25501 gtcaggtaac ctattttata ggtatctacc acaaattccg aacctttgat aatgcctaaa
    25561 ttacgtagtt tctgaatccg ctgatgtatg gcggccccgg aaacattaca ttcacgtgct
    25621 acttcgagaa acggtattcg agcgtctttc gatattagtt gtaatatttt tttatccaac
    25681 cggtcaattt gatgatgtcc catttttggt ttaagttttt tacaggttaa ttttaaatag
    25741 aattatttaa cagacaaaat tatttatttt atttgatttt tcctaataat atttagaatt
    25801 attacatttt cccataaaaa gtttttttta acggtatttt tcattcttgc tatcctccaa
    25861 aatactcaaa taactctgat accttgactg actgatataa tgattttcca atgcttctaa
    25921 aacggcacaa cccggctcgt gggtatgtgt gcaattatta aacctgcatt tgtgtgaaaa
    25981 ttcgaatatt tctctgaaaa aatgaccgac ctcaatttcg ctcatatcaa tagtcccgaa
    26041 acctttaatt ccgggtgtat caataattcc accgcctccc ggaagcgtga acatttcaga
    26101 gaaagtggtg gtatgcattc ctctgttgtg gtactcggaa attacgccta cacgaatcgt
    26161 tgattcagga attaactgac tgattatagt ggacttgcca actcccgagt tacctgaaat
    26221 aagggtaatt ttaccggcaa ggaattgctg caattcgtcc agagtctccg gatgaagcgc
    26281 cgaaacgaga aaaaccggat aaccgattat ttcatacagt tttttcagac tctttgcata
    26341 ctccaattca gcaacgttat accggtctga tttattgata attatgcaag ccggaacgcg
    26401 ataggcttcg gctgtagcca aaaaacggtc aatgaaaaca gaagatgtct cggggtagtt
    26461 aactgtgaca accagcgcag tcagatccag attggcagcc agaatctgag agaatttgga
    26521 tagattcgat gaccggcgga tgatgtagtt ttttctgtcg catatctcat gaattactgc
    26581 cgatccgtcg gctgccgaag aaaattccac tctgtcgcct actgcaatag gattggtgct
    26641 gcggatatct ctgattcgaa gatttcccct tattttacaa tccaccagtt gaccatcgtc
    26701 ggttttcacg gtgtaccagc tgccggtatt ttttactaca agtccctgca ctataatata
    26761 aatttgaaaa tggaaaaata aaagcgatta tttcaaacaa ccgcttttgt gtaacccgca
    26821 tactgcaggt tggtttgtgt aaattgtgta attcagaaaa aaacagttac acggtcataa
    26881 tttctttttc tttttcaagc agcaattcat ccactttccg gatgtattta tcgtgaattt
    26941 tctgaacttc ggtttccgca tctttttcca catcttcggg aagtccctct tttaccattt
    27001 ttttgaaatg ttcgattgcc tcacgtcgtg cattccgaat gcttatttta gcttcctcgc
    27061 cttcagagcg cgattgtttt accaattgtt tacgtcgttc ttcggtgagt ggcggtatgc
    27121 ccagccgaat gacttctccg ttgttaaccg gtgttatacc aacatccgaa ttcataattg
    27181 ctttttccaa aacatgaatc atcgcttttt cccagggttg gatggcaatt gtttttgcat
    27241 cgggagtggt aactgtggct acgttggtta tcgggaccat cgatccataa taatctaccc
    27301 ttatcccatc cagaatgcgt gtattggcct ttccggcccg aatatgagca agtgcttcgt
    27361 ccagataccc gatagcagca ttcataaatt cttctgcttc ctgcattatt gcttttgtgt
    27421 cctgcatagt ttttatgttt tgatttttta ttcgttattt tttaaagcct tgttttgtgg
    27481 taaagttaat ttttcaccag cgtaccaata ttttctccca tcacaacttt tttcaaattt
    27541 ccaactgtat ccatatcgaa tacgtatatc ggcatactgt tttctttgca catggtagta
    27601 gctgtcagat ccattacttt caggttacgg ttgtacactt catcgtatgt gatttcgtca
    27661 aatttcactg ctgtaggatc tttttcagga tcggcggtat atacgccatc cacacgtgta
    27721 ccctttaaca tcacatccgc ttcaatttca atggcacgca gggctgaagc tgtatcggta
    27781 gtaaaataag gattgctcgt accaccggca ataattacta cttcgcctct ttctaaacat
    27841 tcaacagcgt aacgtttgct gtaatactcg cctacaggtt ccatacgtac tgatgtaagt
    27901 acccgtgatt ttgttccatt tgcctgtagg gctgaattta atgccaaact gtttattaca
    27961 gttgccagca tacccatctg atcgccctgc acccggtcga atcctttggt tgcgccgctt
    28021 aatccccgaa aaatattacc tccacctatt acaataccta tttgtacacc aatgtctgca
    28081 atttgtttta tttgtgtagc atattctttg agcctgagtg tatcaatacc ctgttttgtt
    28141 gctccggcaa gcgattcgcc acttaatttc aacaaaatcc gattatattt tatcattggt
    28201 cgttttattt tacaaatgag taacaaaagt acataatttg atttggattt gcaaatacaa
    28261 gaaatcagag atttaatttg taaaaaaaaa tgagaacaaa ccaaactgat ttgttctcat
    28321 tctcattcta ttgataactg atttgttatt ctgctttctc ttcagccggt gtttcggctt
    28381 tcggttcttc cttttttgcc tcaactttcg ttgtttttgc tttgggcgct tcagtttttg
    28441 cttcagtttc agcggctttt ttggatgaac ggcgtgtacg ggttgttttc ctggatgttt
    28501 tatctttcag catgttttcg ttgtagtcca caagctcaat gatacacatc tcagcgttat
    28561 ctcccaaacg gaatcctgta cgaagtatcc gggtgtatcc tccgggacga tcggcaattt
    28621 tcactgccac atcgcggaaa agttcggtta ccgcttcttt gttctgcaga tagctgaaca
    28681 ccgtacggcg tgagtgtgtt gtatcatctt tggctttggt aagcagcggt tctacataca
    28741 ctcgcagtgc ttttgctttg gccagcgtgg tagctattct tttgtggcga atcagtgaaa
    28801 cagccatatt ggacaacagt gcgtgtctgt gtgatgcagt acgtcctaaa tgattgaatt
    28861 tcttattatg tctcattttt attcttactc tttatctaat ttatatttag ttacatccat
    28921 accgaagttc agattcagac tttccagttt ttcttccagt tcggtcagtg attttttacc
    28981 gaagttgcgg aatttcaaca aatcatgacg atgattgctt accagttctc caagtgtcgt
    29041 aacgtcagct gctttgaggc agttgagtgc gcgtactgag agttccagat ctgtcagttt
    29101 ggttttcagc aactgacgca tgtgcagtat ttcttcatca aattcttcta ctccttcacc
    29161 ttcaggaact tccagtgaaa tcttctcatc ggaaaatagc atgaagtggt gaatcaggat
    29221 cttggcagct tctttcaaag cttctttagg atgtattgat ccatcggttg ttatctcaat
    29281 aaccaatttc tcatagtcgg taacttgttc tacacgataa ttttcaaccg tgtatttcac
    29341 attgcgtatc ggagtataga ttgcatcgat ggggataaca ccaatttcgg aggttgacag
    29401 ttttacttca tcggcaggta catacccgcg tcctttactg atagtaatgt caatattaaa
    29461 actggctttt gaatccatcg tacaaatcac gagttcggga tttaacacat cgaatccggt
    29521 caggtattta cccagatcgc cggctttgaa agtggtggtt cctttaactg taatggttac
    29581 tttttcgttt tcaatttctt caactatttg tttgaaacga atttgcttca aattcagtat
    29641 gatattggta acatcatcca acactccggg tattgatgca aattcatgat ctataccttc
    29701 aatatgaata acatttatcg cgaagccttc gagcgacgaa agtagaatac ggcgcaaagc
    29761 attaccaatt gtaataccat aacctggttc taacggacga aattcaaatt ttccctgaaa
    29821 gtcgtcagct tccaacatga ttaccttctc gggtttttga aatgctaata ttgccatggc
    29881 ttcacttagt ttttagagta caactcaacg attaactgtt ctttaatatt ttcaggaata
    29941 tcatcacgct ccggaatatg gaggaaagta cctgacatgg ttgaatcaga gtattcaatc
    30001 cagggatatt tactgtgatt aaatccgctc agtgaattga ggataacttc catcgatttg
    30061 tttttctcac gtacagccac tacttgtccg ggacgaacat tgtatgatgc gatattcacc
    30121 accttaccat ctacggtaat gtgacggtgg ctaacaagct gacgtgcgcc tgctcttgtg
    30181 ggagcaaatc cgagacgata aaccacatta tccagacgtg attcaagcaa ttgaattaat
    30241 attacccctg taataccttt agtgcgttta gctttttcat acgtattacg gaattgtttt
    30301 tcgagcaaac cgtaagtata cttagctttt tgtttttcgc gtaactgaat tccatattcg
    30361 gaagtttttc tgcgacgtga gtttccgtgc tgtccgggag gataattctt tttagacaat
    30421 actttgtcag gtccgaaaat aggctcacca aacttacggg caattttagt ttttggtcca
    30481 gtatatcttg ccatttttta taatttaatt tttatttaat ttttgtgttt cattgtgctg
    30541 attttcatgt ttctttttcg acagccgcga ctgcagagag gtttgaaaat gcagtcatat
    30601 caaaaaaaat ccgggaaaat gcttccagct gaaacaagct gtgattacac tcttctgcgt
    30661 ttgggaggac gacaaccgtt gtgtggaagt ggagtaacgt caacaatttc tgttacttcg
    30721 atgcctgcac catgaatggt acgaatagca gattcacgac catttccggg tccttttacg
    30781 taagctttta cttttcgcag accgagttcg aatgctactt ttgagcaatc ctgagccgcc
    30841 atctgcgcag catagggagt gtttttcttt gaaccgcgga aacccatttt gccggctgat
    30901 gaccaggaga ttacctgacc ttcggaattt gcaagggtta caatgatgtt gttgaaagat
    30961 gaatgtacgt gtagctgtcc tacaccatcc acttttacta cacgtttctt ggctgcaact
    31021 gttttctttg ccatactttt ttcttaattt ttttgctttt ctaaaattcg caaaccgtta
    31081 actacctttt acttcgttgc ttttttcttg tttgcaactg ttttcttttt ccctttgcgg
    31141 gtacgagcgt tgtttttggt actttgacct ctcaacggaa gaccgatacg gtgacggatg
    31201 ccacgataac aaccaatatc catcaatcgc ttgatgttta attgagtttc cgaacgcaaa
    31261 tcaccttcta ccttaaatcc ggttccgatg atttcacgga ttttagctgc ttggtcgtca
    31321 gtccagtctt ttaccttgat gttgatatca acaccggctt gttccaaaat tttttttgca
    31381 ctactgcgac ctattccgta gatataagtc aatccaactt cccctctttt gttttggggt
    31441 aaatctactc cgacaattct tatagccata aactattttt ttatttagca attaaataaa
    31501 ttatccctga cgttgtttga acttgggatt ttttttgtta ataacataca agcgtccttt
    31561 tctacgcacg attttacaat cggctgtacg ctttttaatt gatgctctga ctttcatgtt
    31621 gttttatttt ttgatgtcag acgtcagatt tcagattttc agattttcac tgtctgttgt
    31681 ctgtactctg gtgtctattg gtctaatttt atttgtatct gaatgatatt cttccttttg
    31741 acagatcgta aggtgacatc tcaactttca ctctatcgcc gggcagaatt ttaatatagt
    31801 gcattcgcat tttgcccgag atgtgagctg taatgacatg tccgttttca agctctaccc
    31861 gaaacattgc gtttgataat gcttctataa tagtaccgtc ttgttctatc gcttgttgtt
    31921 ttgccataaa ttaaaattct ctgtctccta aaacttgttc aatatattca aaacttgaaa
    31981 gtatatcagc cttaccctgt ctaatagcga ctgagtgttc aaagtgagcc gaaggctttt
    32041 tatctgctgt acgtgtagtc cagccatctt tttcaaaaat cagattcctt gttcccaggt
    32101 ttatcattgg ctctattgcg atagtcattc ctgatttgag taacactccg ttgccctttc
    32161 tgccgtaatt cggtacttcg ggcgactcgt gaagctttcg tcctactccg tgtccgacca
    32221 tttctcgaac aacagaataa cctcttgact cacaatatgt ttgtactgca gcaccgatat
    32281 cgccaattct tttaccttcg taagcctgct cgattccctt gtaaagcgat tctttagtgg
    32341 ctcgcagcaa actgatgatt tccggttcta cttcacctac acagaatgta taggcagaat
    32401 caccattgaa accgtctttc aaaactccac aatcgattga gatgatatct ccatctttca
    32461 ggattacttt atctgaaggt attccatgta ctatctgatc atttactgaa gtacaaattg
    32521 aacccgggaa accgccgtat ccaaggaaat taggaattcc accgttgtct ctgataaact
    32581 cgtctgctct gttgttaagc tggagggttg aaactccggg agcgataatt ttagccagtt
    32641 cagccaacgt cttggctact attaaattac tgttacgcag caattctatt tcttcatctg
    32701 atttaagata aatcattttt tttttagaat ttagatttca gatatcagat atcagattta
    32761 tgattagcat ctttttgtct gatatctgct atctgtattc tgacagtcta ataagccgac
    32821 gcacttcctg tacgtccctt tattcttccg gatttaagca atccatcgta atgacgcatc
    32881 aagaggtgac tttctacctg ctgcaatgta tccagaatta ctccgacaag aatcaagagt
    32941 gatgttccgc caaagaactg cgcaaactgc tgattgattc ctaagataga agcaaaagcg
    33001 ggtaatattg caatgattgc caggaagaat gatcccggta aagtaatacg cgacatgatt
    33061 aaatcaaggt attccgctgt tctctttccg ggtttaatac cgggtataaa tccattgttt
    33121 cgtttcatat cttcggccat ttgagtagga tttacagtaa tggctgtata gaaataggta
    33181 aacgcaatga ttagaagagc aaaaaccaga ttataccaaa aactttcgta tcgggtaaag
    33241 tggctccaga atgtttgagt tgcttcagag ttggaatatc ctgctattgc gagcggaata
    33301 aacatgatgg cctgagcaaa aatgattggc attacacctg ctgcattcac tttgagaggt
    33361 atgtactgac gaactcctcc atactgcttg ttgcccacaa ttcgtttggc atattgcacc
    33421 ggaatttttc gtgtgccctg taccaaaagg attgaaaaca tgatgaccac aatcaggatt
    33481 acaatttcca acaggaacat taccaggcca ccaccgcctg aatcggctaa gcgtgaactg
    33541 aattcctgaa tcaaagctcc cggcaaacgt gaaatgatac caatcataat gatgaatgaa
    33601 ataccattcc cgattccttt atcggtaatt ctctcaccga gccacattac gaacatacta
    33661 ccggccatca gtataatggt agaggtagcg atgaacagaa atccaccggg taaagatgaa
    33721 cctgcctgag caacctgaac attcaggttg gccatatatg acggtccctg tagtaccaga
    33781 atcagcacag taagatagcg ggtatattgg ttcattttcg ttcttccact ttcgccttcg
    33841 cgttgtagtt tctgaaactt gggtacagct attgtgagca gctggataac gatagatgcc
    33901 gaaatataag gcatgatacc cagtgcaaaa atagatgcat tttggaaggc tccaccggaa
    33961 aacatatcca gcaatgccat gataccaccg ctggtctggc ttttcaatgc ggccagcgca
    34021 ttggcgtcca cacccggtat cactacgtaa gatccgaaac gatatatcaa cacaaatcct
    34081 attgtggtaa tcaatctgtt acggagatct tcaattctcc agatattttt taaagtctct
    34141 ataaacttct tcatgccatc aaatttttac tacagaacca ccggcagcta caattgcttc
    34201 ttcagctgat tttgaaaaag cattggcttc tacttcgagt tttgcggtta gggtaccttt
    34261 tccaagaatt tttaccatcg cagattttga gatataaccg gcttcgcgca gttcgtttaa
    34321 tcctattttg gtaagattgt tactgtcggc tatcacctga agagtttcaa ggttgatagc
    34381 tttgaattcc acacggttta tatttttgaa tccgaatttt ggtaaacgtc tttggatagg
    34441 catctgtcca ccttcaaaac ctactttgcg tgaatatccg gaacgtgatt ttgctccttt
    34501 atgtcctctt gtagatgtac cacccatacc ggagcccgaa cctcgtccaa ttcttttgcg
    34561 agtttttaca gatcctgctg cgggagttaa attgcttaaa ttcattttat ctaaatattt
    34621 ttaagtcaga atgacgattt ttttacttat tttattactt gaaccaggtg gtgtactttg
    34681 gctaccattc ccagaatctg cggagtagct tcatgttcaa ctacggcatg catttttttc
    34741 aaacccaatg cgtccagcgt tcttttctga tctttcggac agcggattct gcttcttact
    34801 tgttgtattt ttattgttgc catattgctt tcgattttat ccgttaaata ctgtagaaag
    34861 tgatacacct ctgttttgag ctacctgatt agcatcacgc aattcgctta atgccagtat
    34921 ggtggctttc accaggttgt gagggttgga tgatcctttt gattttgcaa gtacatcggt
    34981 aatacccaca ctttcaagta cggcacgcat agcaccaccg gcttttactc cggtaccgtt
    35041 ggttgcaggt tcaatgtata ctctggctcc gccaaatttt gcgatttgtt cgtgaggaat
    35101 ggttccattc agtacaggaa ctttgattag gttcttttta gctgcttctg tacctttagc
    35161 tattgctgcg gttacttcac cagctttacc aagtccccat cctactatgc catcttcatt
    35221 tccaactact acaatagcag agaaactgaa cgtacgtcct ccttttgtaa ctttagtcac
    35281 gcggtttact gctaccagtc tgtctttcag ttccagatcg ttggtagttt tcactatatt
    35341 catttttgtt gccatcgttg attaaaattt aagtccacct tcgcgtgcag cgtcagctaa
    35401 ttgttttact cttccatgat acagataacc gttacggtca aataccacct cattgattcc
    35461 ggctgataaa gcatttttgg caatcagttt tcctactgcc gcagcttgct ctgattttgt
    35521 cactttatct ttgcttccca gcgatgatgc agatgctaat gttacacctt ttacgtcgtc
    35581 aataatttga gcgtaaatct gtgcattact tctgaatacg gttaaacgcg gtctttctgc
    35641 agtgcccgtg attttggtac ggatatgggc ttttatctta cttcttctat ttattttatc
    35701 actcattgtt tcacaagttt acgtagatta tttacctgct gttttaccag ctttacgacg
    35761 aataacttca ccccggaatt taacaccttt gcctttgtaa ggttcaggtt tgcggaatga
    35821 acgaattttg gcacaaatct gacccagcaa ttgtttgtcg atacttctga gtttgattaa
    35881 tgggttttga ttacgttcac ttacggtttc cacacttact tcgttgggta aaaccagaaa
    35941 aatcgggtga gtatatccta atgagagttc caaaacctgt ccctgaccgg atgcgcggta
    36001 tcctacacca accagttcaa gaactttttc atacccctga gaaactccta caaccatatt
    36061 gttgatcaaa gaacgataca atccgtggtc ggcacggctt tgcttgtcgt cttcgttgcg
    36121 tatgatttta cattcgtttc cttcgatttc aacttttaca ttcggactta ctgtctggtt
    36181 caattctcct ttaggacctt ttacagtaat tgtctggtct ttaactgaca cggtaacacc
    36241 tgccggtatt tctattggca attttcctat tcttgacatt cttctttcct ccctgattaa
    36301 taaacgtaac acaacacttc accgccgatt ttgaggtcaa cagcttcttt atttgtcatc
    36361 acaccttttg aagtggaaag aatagcaatt cccaatccat tgagtacacg gggcatatct
    36421 ttatagccta catactgacg gagacccgga gtggatactc ttttcaaatt cttgattgag
    36481 ttaactttgt taaccggatc atatttcaag gcaatcttaa ttgtgccctg aggaccttct
    36541 tctacaaatt tgtagttcag aatatagcct ttttcgtgca atattctggt catttctttt
    36601 ttcaagttgg atgcggggat ttccaccaca cggtggtttg ctttgatagc attccgcaac
    36661 cgggttaaat aatctgcaat tggatctgtc atttttttgt tttttaaatt gatccctact
    36721 cagggacaat atttaacgct ctctcaatcc cctaaaaggg ggcaatataa gagccgattt
    36781 tttatagttt gtctgataat ttttgcttac ataaagtctc cctttcgggg agatttagag
    36841 gggcttttac cagcttgttt ttcttactcc gggtattaag cccttagaag ccatttcgcg
    36901 aaactgaatg cgtgagatgc cgaattgacg catatatcct ttcggacgac cggttaattt
    36961 gcaacggttg tgcaaacgta cgggtgatgc gttttttgga attgcctgta atgcttcgta
    37021 atcgccttca gttttcagct gttcacgttt tgcggcatat ttagcaacca ttgcggctct
    37081 tttcacctcg cgggctttca ttgattcttt tgccattgta tcctgtttaa ttagtttttc
    37141 ttaaatggta atccaaattc tttcaacagt gcaaaacctt cttcgtcggt attggcagta
    37201 gtgacgaatg tgatattcat acccatcagc ttgctgatgc tgtcaatgtt gatttcgggg
    37261 aaaataatct gttcggtaat acccagggta taatttccgt gtccgtccag tttgttttcg
    37321 atacccttga agtcacgtat acgcggtaaa gctacccgaa ccagacgttc gaggaattcg
    37381 tacatacgtt ctccgcgtaa agttacgcgt acaccgatag gcattttctt acgaagtttg
    37441 aaatttgaaa tatctttctt cgaaatggtt tgaacagcct tctgtcctgt gatggttgtc
    37501 atttcgttca cagcagtttc gataattttc ttatcagcca cagctgctcc caaaccctgg
    37561 ttcaacacga ttttttcaag ttttggaacc tgcatcacag tggaatatcc aaattctttg
    37621 attaacgccg gaacgatacg ttccttgtat tcttttctta agcttgctgt ttccatatta
    37681 aatttcttct cctgtttttt tagaaacacg taccagtttg ccttcagtat tcaatttacg
    37741 tcctatgcga acaggtttcc ctttttcggc cggattcaga tttgaaatat gaatggctgc
    37801 ttcttgtttc acaattcctc cctgaggaga tttagcgttg ggcttggtgt gttttgacac
    37861 caagttgata ccttctacga ttgcgcgttg ttttttaacg agaacttcga gtacgcgtcc
    37921 tgttttacct ttatcaacac cggtattaac ataaactgta tcaccctttt taatgtgtaa
    37981 tttactcatc actctatttt ttcattggtt aaagcacctc aggtgcaagt gatataattt
    38041 tcatatttgt tgcacgcagt tcgcgggcta caggaccaaa tatacggctt ccgcggattt
    38101 caccggctgc atttaataat acacaggcgt tgtcatcgaa acgaatgtaa gaaccgtcgg
    38161 cgcggcgtac ttcttttttg gtacgtacaa ttactgcttt tgatacagtt cctttcttaa
    38221 tgtcgcccga gggaattacg tttttaactg ttacgacgat aatatcgcct aaagttgcat
    38281 aacgttttcc ggtacctcct aacacgcgga tacaaagtgc ttcctttgca ccactgttgt
    38341 cagctactgt gagtcttgat tcttgttgta tcataattct tacttagctc tttcgtttat
    38401 ttggattaat ctccatcttt tcaatttact cagcggacgt gtttccatga ttgttacagt
    38461 gtcaccaatg ttgcaatcat tcttttcgtc gtgagcgtga tatttcttgg tcttatttac
    38521 gaattttccg taaatagggt gtttttcttt ccattttact gctacagtga tggttttgtc
    38581 catcttgttg cttgaaacca cacctgttct tacttttctt aaatttcttg tttccatcag
    38641 aatctaatta ttatttgttg atttctctct gacgcaattc ggtagcaaaa cgtgcaatat
    38701 tgcgacgaat atctttaatt tgcaacggat tatcaagcgg agaaatggca tgattcatct
    38761 tcatgcgtga tagatggttt ttttcagcat ctaaacgttc aagaagttct ttgtcagtta
    38821 attcttttat ttcacttgtt ttcattttct taagctatta tttagatgtg taatcatagt
    38881 cgcgtcttac cacaaatttt gtggccaccg gcagtttttg agctgctaat ctcaacgctt
    38941 ctttagctat atcaaaagac actccttcga tttcgaaaag gatgcgtcct ggtgtaacgg
    39001 gagccacgaa tccttcggga tttcctttac ctttaccctg acgggtatca gcaggttttc
    39061 tggtaattgc cttatccggg aatacacgga tccatacttg tccttcacgc tgcataaaac
    39121 gggttacggc aacacgagct gcttccaact gacgacctgt gagccatttg gcttccagtg
    39181 tctttattcc gaaggaacca aaagccagct gatttccgcg ttgtgcgttt cctttgaaac
    39241 gtccgtcctg ctgtcttctg aatttggttt ttttaggttg taacataata ttttaaatca
    39301 aatgcattta ttacctgtta ttttttctct ttttgaaacc ttttccaccg cggtcagaac
    39361 gttcattggc atgatatcct ccacgacctg tttctttggc agatgtaaaa ttaggagcca
    39421 ggtctttttt accgtatact tcaccacggc aaatccatac tttgattcct atcaatccta
    39481 cttttgtgag tgcttcggcg tgagcataat caatatctgc tctgaaggta tgaagaggag
    39541 ttctcccttc tttatacatt tcggaacgcg ccatctcggc accgttcaga cggcctgata
    39601 cctgaatttt tattccttcg gctcccagtc tcatggttga agcgatagcc attttcactg
    39661 ctctgcggta agctattttt ccttccacct ggcgggctac attgttggct acgattacgg
    39721 catcgagttc cggacgtttg atttcgaaga tattgatctg aacttcctta tcggttattt
    39781 tcttcaattc ttctttcaac ttatcaactt cctgtccgcc ttttccgatg atgataccgg
    39841 gacgtgcggt gcaaacggtg attgtaataa gtttcagtgt gcgttcgata acgatgcgtg
    39901 atacgctagc tttagcaagg cgggcgttca gatattttct gattttgctg tcttccagta
    39961 ttgtgtcgcc atagttgttg ccaccatacc agttagagtc ccatccgcgg atgattccta
    40021 aacgattact tatcggattt gttttctgtc ccatcttgct taattttggt tgtcgttagt
    40081 atttttggta tccacgtaca gtgttacgtg atttgagcgt ttgcggatac gataaccacg
    40141 accctgagga gcggtacgca aacgtttgag catggtagct gaatcaacat agactgcact
    40201 tacatataaa ttggcatttt ctgctttgtc ttccactttg gtttcccagt tggcgatagc
    40261 agatttcagc aatttttcca ttttagcaga agcttctttg tttgagaact tcaagacgct
    40321 caacgctttg ttcacttcca tcccacggat catgtccgcc acaaggcgca ttttgcgtgg
    40381 tgatgttgga acattgttaa gcttggcgaa ataaagcgtt ttgttagctt cttttcttgc
    40441 ttcggctgat attttttttc tttgacccat tgtatcgaaa ttttttactt ttttcttact
    40501 ttaaactttt caatggatta tttcttttta ccaccgtgac cgcggaagat acgagttggt
    40561 gcaaattcgc ccagtttgtg tcctaccatg ttttcggtaa cgtacacggg aataaattta
    40621 tttccattgt gaactgcaat tgtgtgacct acaaaatcag gtgaaatcat tgtagcgcgt
    40681 gaccaggttt tgattacgga tttctttccg ctttcattca tggcaagcac ttttttctcc
    40741 aacttcacgt tgataaatgg tccttttttt aacgaacggc tcatattatt tacttaattt
    40801 ttctgattat ttttttcttc tttcaataat atactggttt gactggtttt tcggataacg
    40861 tgttctgtat ccttttgcca ataaaccttt acgtgaacgt ggatgtccac ctgaagcgcg
    40921 gccttcacca ccacccatgg ggtgatcaac cgggttcata gctacacctc tcacgcgtgg
    40981 acgacgtccg agccaacgag aacgacctgc ttttcctgat ctttccagtg cgtggtcaga
    41041 gtttcctaca cttccaacag ttgctttgca ggtagaaagt atttttctga tttcgcctga
    41101 aggtaatttt acgattacat atttttcttc tctcgaagtc aattgtgcaa atgtacctgc
    41161 cgagcgagcc attgaagctc cctggccggg acgaagttca atattatgaa ctacggtacc
    41221 aagcggaata ttctgcaaaa acaatgcatt acctacttca ggagcagctt ctgcgcctga
    41281 aagaactact tgacccactt gcaatccgtt cggagcaaga atgtaacgtt tttcgccatc
    41341 agcataataa agcaatgcaa tacgagccga acgatttgga tcgtattcaa tagctttcac
    41401 tgtagcaggc acaccatctt tgttacgttt aaagtcaatt actctgtatt ttctcttgtg
    41461 accgcccccg atatagcgca tggtcatttt accatcgtgg ttacgacctc cgctttttcg
    41521 tgtgcctgta acaagtgatt tttctggtgc acttgcagta attgtgtcaa atgtaccaat
    41581 aatct
//
LOCUS       DAORKP010000002        38856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12_length_38856_cov_8.641324, whole genome shotgun sequence.
ACCESSION   DAORKP010000002 DAORKP010000000
VERSION     DAORKP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 38856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38856
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_38856_cov_8.641324"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLN75_00270"
     CDS             <1..557
                     /locus_tag="PLN75_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HRG02579.1"
                     /translation="MAEKYGKYPNVIYEIFNEPDYYTWPEVKAYSEELIKTIRAIDPD
                     NIILVGSPHWDQDIHLVAEDPIAGQTNLMYTMHFYAGTHKQWLRDRTDAAMAKGIPIF
                     VSECAGMEATGDGPVDEVEWKAFLDWMEARKISWVVWSVSDKNETCSMLLPRAKSTGD
                     WTDELIKPWGKISREAIRNGNLRK"
     gene            629..2053
                     /locus_tag="PLN75_00275"
     CDS             629..2053
                     /locus_tag="PLN75_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRG02580.1"
                     /translation="MRNKLLVLLILCFVAGSAIAKVTLPDILSDNMVLQQNTGVKLWG
                     KASPNSMVKIQPSWMKMTTTVKTDKKGNWITSIQTPQATYTPQTLTFSDGEVLTLKNI
                     LIGEVWLASGQSNMEMPLNGFWNCPIQGANETIAFSGQNKGIRFATAPKTAAYEPQET
                     VKGKWMESNPENTPWFSATAYHFAQTIYKTLNVPVGIIVCSWGGSRVESWTNREILET
                     YADIDLSKKGIEAVEEWKRPLVMYNGMLKPLTNFTIKGFIWYQGESNVGKHEDYPVRF
                     AKMVELWRKDRGLGNLPFYYADIAPWIYGDGESGISGALLREAQFKSQFIIPNSGMIT
                     TNDLVEEYEKTNIHPKNKTDVGKRLAYLALNQTYNFKGIAARGPEYKSMEVKDGRIIL
                     SFNYAQDGFNRENDIRGFEICGSDKVFLPAEARLDNNNLKIILSNGQIPEPVAARYGF
                     RNFLPGNLANTRELPVVPFRTDNF"
     gene            complement(2324..4138)
                     /locus_tag="PLN75_00280"
     CDS             complement(2324..4138)
                     /locus_tag="PLN75_00280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRG02581.1"
                     /translation="MTFKELNLRAEILSAVEQLGYELPMPVQEQVIPFMLTEQADLVA
                     LAQTGTGKTAAFGLPILNMIDVKRREVQALVLAPTRELCIQISNDMKGYAANMTDVHI
                     VPVYGGEDIRKQLKELDRTPQIIVATPGRLIDLTERGKIQLGNINFLVFDEADEMLNM
                     GFKDDIETILKETPENRRTLLFSATMPAEIQRIAKQYMHDYQEITVGVRNSGTENVEH
                     IYYISQARQRYLVLKRIVDLNPDIYGIVFCRTRQETKEVAEKLMHDGYNADALHGDLS
                     QPQRDSVMQKFRIRNVQLLVATDVAARGLDVSDLTHVINYNLPDDVEIYTHRSGRTGR
                     ANKTGISVSIIHSKEKFKIKDIERMLKRKFEQRQIPNGLEVCKKQLFYQIDKMQNVDV
                     NEEQIDPYMAQIMNQLEYLSKEELLKRFVSLEFNRFLDYYKNAPDLNITEHAPRERDG
                     KTEQNNGKENGNRRNKSGNRVRLKINLGTKEGMNPRRILGIINDITDDKSINIGGIEI
                     TNKFTFFDVFEDQKDRVLSAFEQTQDLNVSVAKGSRSFSNDEDKKDNPRDKRSGEKRN
                     SFRNERKYEGGKHNSSRKRETAYSAGSESDKPWRNRRR"
     gene            4566..6212
                     /locus_tag="PLN75_00285"
     CDS             4566..6212
                     /locus_tag="PLN75_00285"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRG02582.1"
                     /translation="MTKSPLQIARSSYQPKVPISLTGDVAIHEGKVTQSVADQEDIKK
                     LFPNTYGMPLIEFVKGEKSDYPPVNVGVILSGGQAPGGHNVICGIFDGLKKLNPESKL
                     YGFLGGPSGLVDHQYTELTADVIADYRNTGGFDIIGSGRTKLEEEWQFEKGKEICKEL
                     DIKAIVIIGGDDSNTNACVLAEYYKQHDAGIQVIGCPKTIDGDLKNEMIETSFGFDTA
                     CKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNIALISEEIEAKN
                     MTLRDIVDYIVDIIVIRAKSGLNYGTVLIPEGLIEFIPAMKKLIADLNDMLADNDDFN
                     AMTSDEERREYVKGVLNPESTAVYRSLPKGIARQLTLDRDPHGNVQVSLIETEKLLIE
                     MVAKRLAHLKAEGKYKGKFSAINHFFGYEGRCAIPSNFDADYTYSLGVVASVLINAGK
                     TGYMASVRNLTAPASEWIAGGVPITMMMNMERRHGKMKPVIQKALVRLDGEPFKFFEK
                     YRDKWSDETSDFIYPGPIQYFGPSEVCDQPTRTLLLEKGK"
     gene            6612..7448
                     /locus_tag="PLN75_00290"
     CDS             6612..7448
                     /locus_tag="PLN75_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRG02583.1"
                     /translation="MYKQVLRSKLLYVFTLMLVAGILSSCITARKVNYLQKPSGAIPA
                     YKGDIGYEEYNISGADKLYIRVYSPDKDINTIFNGSSSQMMMGGTMQSGSDYTDLYTY
                     QVKNNGAINLPLVGDVFLAGQTIREAKKTVENAIKTTMKDDCSVDVRIVGRYFSVIGS
                     RINGKYPIMREKMNIFQAMAMAGDIGTFGDRSKIKVLRETPNGPQIKVFDIRSKDIIN
                     SEFYYIQPNDVIYIQDVKSQFFSVTSFGSALSTLFSTFSFGMLIYYLAVPEKPAPVTT
                     SN"
     gene            7468..8244
                     /locus_tag="PLN75_00295"
     CDS             7468..8244
                     /locus_tag="PLN75_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRG02584.1"
                     /translation="MFTHQYMKTKWIHALLIILVAIGFSSCYTQRDKSLWQDSKSFPK
                     YAKADYEQYKIHVNDELIFRVTSTDEDFVRLIDAGSTASQQQGISYRVYQDGTIDLPF
                     VDSVKVAGMTINDATEAIQRRFKQIIPDAEVKLALSNKVYTVIGEAGNGIFPIYREKM
                     TIYQALAQSGEIALSGDRKHIRILREKTGETEILEFDIRPKSVINSKYYYIYPNDVIY
                     VQKESASFYKSNNYTTLLGLITSSISLFTTVFYYTKVNNN"
     gene            8292..10682
                     /locus_tag="PLN75_00300"
     CDS             8292..10682
                     /locus_tag="PLN75_00300"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRG02585.1"
                     /translation="MEENKNSNQYTQFSDNEESDFILKEWLMLFLHYWYVFLFFIILA
                     LGAAYLKNRTWIESYQSKGTIIIEENRYSGSQTFMQGFGVQPGYRNVDNQTVILTSYD
                     LISRVIDSIPFLSVDYITKSRFKTRNLYNETPIVINADFIDPKIYGVLFKLSINTDGT
                     YVITDENGSIDKTLNAKGRLGIPIQHNLFFITVEPTSNKILRREIFFRFRSKESLISD
                     FMSRLQIGFIQDGSSVLGISLTSETPDRDIDFINKLCSVFLTENLERKNDAASKTMDF
                     IDQQLDTVSKSLAISEGAMTQFRRSNQIVDLPSHSSEILGKATQYDEKQSALNLRDSY
                     FKYLTNYLKTNLDAGAVVAPSSLGLNDPMLMSLVQQFNDLIAKRNETSDKNPLYAKYN
                     REIESLKNSINEVITNMRASMEIEGQDLSRKMASVQSDISALPRKEMQLIGIERKYRV
                     DDNYYTFFLQKRAEAAIQKASNSPDNSILDKARIVALTNGNTKSRTILIYLLIGFLIP
                     ALFVVLKELLNNTIRDTKDVERSSKYLLIGTIKHTKNTDPTLTSKRPRSSFTEMFRVI
                     RTRIEFIVQRKTKIAILVTSAESGDGKTYFSTNLASTYGMTGLKTLLIDMDIRKPSIN
                     ERMGLEETDGVTNYLIGEKKLDEIILKKEELNFDILLAGSVPPNPGELIRSDKLKNMF
                     LELREKYDYIIVDTSPIGLVADAYSLAPMMDANLIVVRSEKTNKSFFRKLNDQLISDN
                     LKHFYIILNDINIENTSYGRYGRNYGYGYGYGYGYGYSKEKDAQKYVQYYEDDKDI"
     gene            complement(10873..13149)
                     /locus_tag="PLN75_00305"
     CDS             complement(10873..13149)
                     /locus_tag="PLN75_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRG02586.1"
                     /translation="MDPIYLIIVIILLGLAVMDLAVGVANDAVNFLNSSIGSKVAPLW
                     LILTVASLGVLIGSLFSSGMMEVARSGVFHPEKLTFHAAMILFLAVMLTDIVLLDVFN
                     TFGLPTSTTVSLVFELLGAALAVSLFTIWSNDSGSLGEYINSGKALAIISGIFISIAI
                     AFVFGSLIMYISRLIFSFNYKKSFVYLGAVWGGIALTAITYFAIFKGLKGSVLVSEAF
                     LDFLNNSMGKAILYAFLGWTVLMAIFQFVFRVNILKIIVLAGTAALALAFAGNDLVNF
                     IGVFMAGLDSFTIAKDVVASGGSIDTLYMGELNKPVTAQWQWLFGAGIIMVLALWFSK
                     KSRTVTETEVNLARKGEGVERFSSSALSRSIVHGAMKISKAIDKSTPPNVKEFLDKRF
                     EPVAIENNASFDLIRASVNLTISALLISLGTSLKLPLSTTFVTFMVAMGTSLADRAWG
                     RESAVYRINGVLTVVSGWLLTALVALSASLLVGLFLMWGGKIAIFVMLALVGFLLFRS
                     GKLHTKRKEKEESKHVQLSSEKQLVAECTSEVKTVLEATLNTFSLTIDGLKNENRKTM
                     RNVVAETNSLYEKYKDKRMYEVVPTIETIQISALDLEQEYVQLVDYSFEITKSLKAMA
                     ESSYSYIDNNHTPFSKEQIDDLKQMYNALTDSFGKFSLMLESNDYSGFDQITQLRDLM
                     TDLYVKLTKRQIKRVKENETTTRGSILFLNLINESKIIALQSSNLMKSQRSFSQQYSK
                     VAREMSTADIVKSIPLTM"
     gene            complement(13412..14515)
                     /gene="meaB"
                     /locus_tag="PLN75_00310"
     CDS             complement(13412..14515)
                     /gene="meaB"
                     /locus_tag="PLN75_00310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRG02587.1"
                     /translation="MHYHHKHPENDPQFKSLNVNGGVTDVPVINPYSKKRIKRRRLSA
                     GEYLEGILAGDVSVLSQAVTLVESSLPHDQEIAQEVIEKCLPHAGKSIRIGITGVPGA
                     GKSTFIEALGMHILQTGHKLAVLAIDPSSERTKGSILGDKTRMEELSVAKNAFIRPSP
                     SAGSLGGVARKTRETIVLCEAAGFDTVFVETVGVGQSETAVHSMVDFFLLIQLAGTGD
                     ELQGIKRGIMEMADGIAINKSDGTNVEKSKLARQQFKNALHLFPMAESGWSPEVINCS
                     AIEKTGIADVWQMVINYIGFVKNNGYFESKRTNQSKYWMYETINEKLKSGFYNNETIQ
                     KLLQIAENKVLRNEQSSFIAAKQLLENYKKLSD"
     gene            complement(14536..15639)
                     /locus_tag="PLN75_00315"
     CDS             complement(14536..15639)
                     /locus_tag="PLN75_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRG02588.1"
                     /translation="MIRKLFSIVAVSVVFFTASVTAQKAEINFREKTFDFGQITEDGG
                     NVSHVFEFTNTGTTPLVIQRVNASCGCTTPEWTKTPIEPGKKGNVTATYNPMGRPGAF
                     NKQIYVYSNSTEEMVSLIIKGDVKPKSSSAQSAPQANNYPIQIDALKLNSKVVQMSNV
                     DSKSTQTRTIAMLNSSPSNLTVTLPELPAYLSANITPKTLKPNEQGAITFTFDGTKAS
                     EWGPVDDKLTLTLNGKKITGDANKITVFANVTEDFSKMSAEQKRLSPIMEVKSSNLYI
                     GNIKKGNRVRGKVNIKNAGNNPLEIRRITNNNSDIIIHPLRATIKGGHTESFKIDVDS
                     KFLPKGEYKKAFTVQTNDPVNTFLTFTVSYKVI"
     gene            15821..17008
                     /locus_tag="PLN75_00320"
     CDS             15821..17008
                     /locus_tag="PLN75_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRG02589.1"
                     /translation="MLPDKFKALSEAVFPYVVDCYRHLHAHPELSFLEFETSDFITNQ
                     LKSMDIPFRQGIGGTGVLGVITGNNPAKKTIALRADMDALPISEETGLAWKSLNNNVM
                     HACGHDAHMACLLGAAKMLNELKNEFEGTVLLVFQPGEEKAPGGASLMLKDGVFDTLT
                     PELIIGQHISEDYPTGTLAFRSGVVMASADEIHLTVKGQGGHGAKPHLCNDTVLAAAH
                     TLVALQQVRSRLCPPLTPMVLTFGKLIANGETNVIPGEVKLSGTFRTFDEKWREEARG
                     HIGRIIHDTCSAFGCAVDIEMPEGYPCLINNEEINCKSKQYASELLGENNILEFDQRL
                     TSEDFSFFSQRYPACFYRFGTRGESNQHMGRSHTSGFCIDEKSLLTGTSGLAWIALGF
                     LNG"
     gene            complement(17034..17567)
                     /locus_tag="PLN75_00325"
     CDS             complement(17034..17567)
                     /locus_tag="PLN75_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG02590.1"
                     /translation="MDFIRILSSADKYADELTELYLGAFSENQRHTKEDFRHLLDTNK
                     QFNCNAVLMNNQLIGFFHFWDFDDFLFLEHIAIEPPLRGHKLGEKVIKLVRSLAAKPL
                     VIEVEQAENSVFGARRIEFYRRLGFEIVPFEYLQPPYRVGEDYIPLEMMSDDLEFASE
                     NFEKIKAKIYRQVYGIG"
     gene            complement(17706..18299)
                     /locus_tag="PLN75_00330"
     CDS             complement(17706..18299)
                     /locus_tag="PLN75_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRG02591.1"
                     /translation="MKITNNAVVNAEYELYVDGENGELELMERATSEQPLNFIYGIGM
                     MLPKFEQNLNGLETGDSFDFTISNEEAYGPYDDEAVIELERAVFEIDGKLDEEMVFEG
                     NVVPLMDSEGNRLQAQVVSVNDTHVTVDLNHPLAGENLHFKGKVLEVREATEEELNAL
                     LGGGGGCGCGGNCGDGCGDDCGCDDDHNHSHGCGCGC"
     gene            complement(18397..21108)
                     /locus_tag="PLN75_00335"
     CDS             complement(18397..21108)
                     /locus_tag="PLN75_00335"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRG02592.1"
                     /translation="MLQYANGQQTNTNPVPVPLSVNQPADTLKKPAPNDTTKKKAEQF
                     DAEITYSASDSIVFFGNGTGFLYGGTDVKYKEMNLKEANLVRIRMDSSMIYAYSTVDS
                     TGTLSGKPIFKDEDAEYTSNEISYNFKLKRGYVKQAVTQQGDGFIISNKTKIIDNDIL
                     NIAQGKYTTCDLHDHPHFYLGLSKAKVKPGEYVVTGPANLVILDIPVPIALPFGFFPF
                     NTNYSSGIEMPSFDTEFSRGFGLTNGGYYFAINDYMDLSIKGDIYLKGTWGVVAQTNY
                     LKRYKYNGGVSITYREDVTGEKDLPDYNKSKNFSIRWSHSQNPKVNPFRTFSASVNFS
                     TSGYNRSNINSYYTPVNAENTKSSSVSFSQRFPNNPFSFTANLGIDQRTRDSTISLTL
                     PNISISMSRIYPLKKKNRIGKERWYEKISMSYSGTISNSITAKENKILQSSLANDWRN
                     GMQHSIPISASFNLLDYITVSPSFSYRERWYMYSIERAWDEAKQRTVDTRVNGFKRAY
                     DFNMNVSANTTLYGIYTPSRKLFGDKVIAIRHKLDPSVGMGYTPDFGDPRWGFWDTYM
                     KTYADPKDPTRKLMEEVHYSRFETSMYGGPGRGKNGNVFFSLSNNLEMKLRNDKDTTD
                     TKPTKIVSLIDQFAISSGYNLAADSMQWSNFSANLRIKLGKAYTLSLSGAFDPYMYGV
                     GPDGKSVRRINQLRWNNGKFPKFMGTSLSYSYTFSNETFKRKEKKGGKTDGAQSESDK
                     GAYDEDPNNPNQDHTLNVQNKGTNQNLENSEADADGYEKLTIPWSLSVNYSVRLGDGD
                     KFDYEKLEYNLRLTHNLNFSGNIQLTPGWQISGTTSYDFEAKQFTYTSFNVTRNLHCW
                     TLTGNFVPFGPYKTYNFRIGVNSSMLQDLKWEKQGNRESIQNKTVWY"
     gene            21381..22478
                     /locus_tag="PLN75_00340"
     CDS             21381..22478
                     /locus_tag="PLN75_00340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRG02593.1"
                     /translation="MKSLYKITGAIIMLMIISTAFLFAQKPFTVVIDAGHGGHDSGAV
                     GKYSKEKNINLSVALRLGSMIKENYPEIKIVYTRTTDVFLTLQERADIVNDNKADIFF
                     CIHTNATGGSSAHGTETYVLGLHKTKSNLEVAMSENSVMMLEDDYETRYQGFDPNSVD
                     SYIIFEFMQDKYLDQSLRIASLIENQFRHANRYSRGVRQAGFWVLHKSAAPSVLVELG
                     FISNADEEKFLNTDEGKHQLTKAIYNAFEAYKRDFDKKTGISVTPKEMTVYKADLKKD
                     SVDENQVIYKIQLFALENKINPNGREFKKLNDVNYYREGKFYKYTYGEETTFEKIEAL
                     RKSIADKFPYAYVIAFRGNQKITVEEALRRK"
     gene            22527..23429
                     /locus_tag="PLN75_00345"
     CDS             22527..23429
                     /locus_tag="PLN75_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRG02594.1"
                     /translation="MRKKYTREVKIGLMVIVAIFILYFGLNFLKGIDIFQKQSSYYGN
                     FENVDGLVKSSSVKVKGFKVGQVSDIQYDFSKKESFKVVITVSEDIKLPVGTTMLLTD
                     EGLLGGKFIELVYSPVSDKQFTHHKNDKLPTQTSSGLMAGLTGSLMPKIEQVIQQADS
                     LIRSVRTLSESTQITNSLNSLENTTADLAITSAQLKGLMSNQMPAMMNNINAVTGDIR
                     FVSENLKKVDFAGTMENVDFTIKNLNDFTRKLNSNENSLGMLINDKNLYINLNNTAES
                     ADKLLIDLKENPKRYVHFSLVGKK"
     gene            23827..25221
                     /gene="dnaA"
                     /locus_tag="PLN75_00350"
     CDS             23827..25221
                     /gene="dnaA"
                     /locus_tag="PLN75_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRG02595.1"
                     /translation="MSRHEQLWNSCLKIIKDNINEASFNTWFSPIVPVRYENKVFVLQ
                     VPSQFFVEYIEEKYIDLLRMTLTRVAGSGTKLEYRVLIDKSSGTGTQIPSTAEQKPKQ
                     FGSNSAQSTFVSPYQRLQLPELDPQLNPSFNFNSLIEGKSNKLARTAGLSISNDPGKT
                     IFNPLFVYGKSGVGKTHLVNAIGVMTKQLHPEKRVLYVSANTFQIQYTDAVRNNTQND
                     FLNFYQTIDVLLIDDIQEFSGKTGTQNTFFHIFNHLHQTGKQLVLTADRSPLEMVGIE
                     QRLLTRFKWGLSAEIEKPDFELRKSILKSKIYRDGLEIPENVVDFIAENVVDNVRDLE
                     GVLISLLAHSTLTNMPIDIILAERVISRIVSITPKVMSIEFIRDTVCDFFKLSVDAIS
                     TKSRKLEVVQARQIAMYLSKNLTKNSLSTIGNYIGQRDHATVLHACKKVMDLMDTDKH
                     FKKNVEEIEERLKK"
     gene            25370..25936
                     /locus_tag="PLN75_00355"
     CDS             25370..25936
                     /locus_tag="PLN75_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02596.1"
                     /translation="MIFKFTILSDEVEDFVRIIEIDSESSFLDLQNAILDSVDYETNQ
                     MTSFFLCNDDWEKEQEITLVEMESSSEYDNLVMEDTLLDEMLTDEKQKLMFVFDIISE
                     RAFFIVLTEMIPGKDLDEAVCTTSTGNPPVQISEDFDMNIAPGKTYQIHSDENFYGDE
                     EFDLDELDEEGFGDLNFDDSTLFDDPKA"
     gene            26012..26917
                     /gene="miaA"
                     /locus_tag="PLN75_00360"
     CDS             26012..26917
                     /gene="miaA"
                     /locus_tag="PLN75_00360"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRG02597.1"
                     /translation="MATLIVILGPTGVGKTNISLRLAEKFACPIVSSDSRQFYRELKI
                     GTAAPSEDELKRVKHYFIGTHSVFDEYNAGQYELDAVSLMDELFLKHDFVLLTGGSMM
                     YIDAICKGFDDIPSVKPEIREFWQKEYAAKGLEHIQNELKRLDEKHYHEVDLKNPKRI
                     MHALEICSMTGRPYSELRTGGKKMRNFRILKIGLTRPRAELYERINKRVDQMVEQGLV
                     EEAHRFYPHKHMNTLNTVGYKELFAHWDGEYDLEHAINLIKQDSRRYAKRQMTWFNRD
                     TEINWFHPDEEEKIIHFITSNTSGK"
     gene            26928..27941
                     /gene="murB"
                     /locus_tag="PLN75_00365"
     CDS             26928..27941
                     /gene="murB"
                     /locus_tag="PLN75_00365"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRG02598.1"
                     /translation="MKILENTSLKSYNTFGIDVKARFLAEYNSVDELKELLQSETARQ
                     NRLLHIGGGSNLLFLNDFEGIVLHSEIRTIETCEEDAESVLLRVGSGVVWDNFVEYCV
                     DKNYYGVENLSLIPGEVGASAVQNIGAYGMEVKDTIEKVEFIEPESLNSRIFTNKACN
                     YGYRNSIFKGELKGKAIITHVYFRLKKQVCFVLDYANLKESVERNGEINLRNIRQTVI
                     EIREKKLPNPTLLGNAGSFFMNPVIPKNQFEMLLLNYPTMPHYPVSDTAVKVPAGWLI
                     EQCDLKGKKFDRAGIYEKQALVIVNLGNATGHDIATVASLVQISVKKRFGIDIIPEVN
                     YIS"
     gene            complement(28011..28934)
                     /locus_tag="PLN75_00370"
     CDS             complement(28011..28934)
                     /locus_tag="PLN75_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HRG02599.1"
                     /translation="MKLKFFLIALMPLIILSACNTTRQVSTGKEAFTPEMQKIMTGYP
                     EITGFERRAIFLEKLTPEQEQQQKIEIFPGRELLTDCNRYGLQGQMLSKTFQNGHSYF
                     LFNTNGEIYSTKMACPDESKVPAFVTGESVITDYRSDVPLVVYISRYLQVKYCLWKAG
                     DEKSVALNGNGTLASEEALQNTNYFPEKAGYVKHILYLPELEAADEINRKVEIIPGLL
                     RKVDCNDHRLNGTFESGQVEGFGFDYWIFQSDGTYSSTKMNCRDKIMSEKFVSSEKKL
                     MAYNSRLPIVVYTPSGFEVNYKIWETNGKLY"
     gene            complement(29044..30279)
                     /locus_tag="PLN75_00375"
     CDS             complement(29044..30279)
                     /locus_tag="PLN75_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRG02600.1"
                     /translation="MKNNDIRVDLEIELESNLFQTLEGKLVEKIIAEANYEPEENIWK
                     NILEGHSFKVTEKMSPKLYHIFHDVKNKLNYKKNIDFYITNSAELNAFALTRSDDKDT
                     DIININSALISMFDDDELRFVVGHEIGHLITRYARVVRLINFIFPDPEKVPLILAHRI
                     ELWQKVSELTADRFGFIASPNVEKCVSGFFKMASGLSTDRIDFDYKAYLIDNEKILEY
                     FTQNRNMFTHPVNPLRIKAIKLFSESSLYGDFKNDKEFNTDQVLNAEMDKLIAMLQTI
                     STSPMDAFRKQFLASAGLIMANIDKNINDDEYESILESLSNYTVFPHEYLSEICNTST
                     EEVFKSSISNIIQQNPAERFQLLQYMANIAMSDNSIFNKEINFLFEVGESLLGLTRRE
                     IAQILAATLQQGFIPELYR"
     gene            30536..31954
                     /locus_tag="PLN75_00380"
     CDS             30536..31954
                     /locus_tag="PLN75_00380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRG02601.1"
                     /translation="MKVKFIGAAREVTGSKTLITTDDGKKILLDCGMFQGKGLETDEL
                     NRRTGVNPAELDHIILSHAHIDHSGLIPYFYKLGFRGSVICTTATRNLCSIMLPDSGH
                     IQQADTEWFNKKRARQNLPRVEPIYDLEDAEQALKLFIAVDNNRRFYINDKINVKFTN
                     TGHMLGSSVVNLEITENGKKIRLAYTGDIGRPHNRILSGPVPFPQCDYLITESTYGNR
                     LHETEKQTEEDFLKIITETCVVNKGKLIIPSFAIGRTQEVVFVLNKFFNDGLLPRIKV
                     YVDSPLAVNATDIFRMHLDLMNEDVKREIKEGDHDPFGFNNLIYIKKVEQSKELNELN
                     EPCIIISASGMAEAGRIKHHIANNISNPHNTIMLVGYCSPTSLGARIQEPDLKEISIF
                     GKMHDVKARITRMEAFSGHGDYNEMKSFLECQKKSAVKKTFLVHGEYEVQQFYREILS
                     KDGFKNIEIPAYGNEFELNQVN"
     gene            32034..33134
                     /locus_tag="PLN75_00385"
     CDS             32034..33134
                     /locus_tag="PLN75_00385"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRG02602.1"
                     /translation="MFKKLILPIMFCMATAGFSQENYNMLVGTYTRGTVSKGIYSLTF
                     SGKGKFISQKLLAESDNPSYLAFSPDRKFVYAVNEHGEESTISAFAFDKANQTLTFIN
                     KVPAAGADPCYITVSDKHVFTANYSGGSISVFHRLPNGKLSEASQVITHPRKNFGNRR
                     YGPSNAHQIIFSPDGKYLMATNLGTDRVFTYKYNPESDDEVLSYVDETGVKRASGPRH
                     LAFSRNGKFLYLVQELNAGITVFSVSDDGKLSVIQETTLVTDSTKKNGAADIHLSPDG
                     KFLYATNRGEANTITCFLIKNDGKLRFVEEYSTFGNAPRNFTITVDGKYLFVGNQKTN
                     NITVYIRNRCSGKLKLLDDKTELGAPVCLVLY"
     gene            complement(33210..34334)
                     /locus_tag="PLN75_00390"
     CDS             complement(33210..34334)
                     /locus_tag="PLN75_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRG02603.1"
                     /translation="MKTFKHLFIFSIILLFFLSCGKKTENGNELSENADADLIEISTE
                     QFNTNRMQLGEATLQTFSDEVACRGFLFAPVNGIAKVSTPIAGTVENIRFKTGDFVRQ
                     GQVVCSVSGSEFLSLQQQFAESSAGFQKAKLDYERLKALRADKIGAEKDFTASESLYK
                     ASLASYNALKTRILALRLNPSQIENGQMYTSFPVTSPISGFITKTGAVIGQYVEMQNE
                     IAEIVNVTSLQLQLSVFEADVHKLRAGQRIRFGISGNSSQNLRATLIALGKTINPDSK
                     TIDCIARIDETDKSKLINQSYVEAIITVNNKDAKAVPESAVQKQEKEYFIYVLDKKEG
                     DKFFLKKTKVETGKSNKNFIEILSGLPAEKEIVLSGVETL"
     gene            complement(34409..38758)
                     /locus_tag="PLN75_00395"
     CDS             complement(34409..38758)
                     /locus_tag="PLN75_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRG02604.1"
                     /translation="MLEKIIGYSLKYKLIVVLFTFAIVVFGFYAIFNIPVGAVPDITN
                     NQVQVITTSGNLSTQEIEQFITAPVELEMANLPGVKEIRSVSKFGLSVVTIVFKESMG
                     TYLPRTLIAEKIKSASAKIPEGFGTPEMGPITTGLGEIYQYVLDVKPGYEDRYSPMDL
                     RTIQDWIVRRQLSGIDGVVEVNSWGGYLKQYEVAVNPSKMQALGVTLMDVFGALENNN
                     SISGGAYIEKKSESYFIRGDGQVRTLDDIQHIVVKNQGGIPVMINDVAQVHFGYANRF
                     GAITANGEGEKVLGQIMMLKDANSKRVISEVKKRVDEVQKSLPEGVFINPILERSELI
                     GKTTLTVLENLLFGCIIVFLVVLLLLGNLRSALVIASMIPLALLFTLSMMFVFGIDAN
                     LMSLGALDFGIIIDGAVIIVEYIVVQLTADKLKFKDISAEERQNLNNRITQTSASKMM
                     QSAIFGQVIILIVFIPILSLQGVEGKMFRPMAMAFGFAIIGAMIFCLTWLPVISSLFL
                     RPLDLNKKTLSDRLLGFARATYEPVLKWSLNKKVLIFTLSFLTLIFSVFLFSRMGGEF
                     IPTLDEGDFVIQPVLKTGTSLTKTIELTTQMENILMDKFPEVEKIVCRIGAAEVPTDP
                     MSMEEIDMIIRLKPKDTWTSAKTKEELADKFKEALSVIPGIDYEFTQPIEMRFNELIT
                     GVRADIAVKLFGDDLDYLSQKAEEIKHLIEKVDGAGDVILEKTTGLPQMNVTYNRSKI
                     AYYGTDIRTLNSYLAAAFGGEIAGNVFEGERRFDLVVRLQQAFRTDIDNIRQLPVALP
                     NGIQVPLSELAEINFSEGPAKISRENTHRRVVVSVNVRNRDLQSVVEDIQQLVDKNVK
                     LNPGYYISYGGQFENLQNATKRLVFAVPIALAMIFIFLHFAFKSLKDTILIFTAVPLS
                     VVGGIVALWIRGMPFSISAGVGFIALFGIAVLNGIVLIEHLKHLKETGMKNMDELVKK
                     GTSDRLRPVILTAAAAAMGFLPMAISSGAGAEVQRPLATVVIGGLITSTMLTMIVLPL
                     LFKVFHDDEGIKYKIVRKLKSKNFIIPLLLALSGTLSAQTPVYSLDECMNLALKNNRA
                     LGAYLLKIDERKALASRVMELDKTTFYYGYDQANVAENGHPLRTLGVEQTFLFPTIHS
                     LNNKMNRMEVTQAQLELQKQQLILKKKVASSYYSYQFLLKKQRYFNRIDSMYDRLNSM
                     ADTQLRLGDISELDKLNLQAKKQQSALEISKIAIEIQHAYAGLKSVMQTGEDFRIEEI
                     PMKIITVSKPDMQTSPEIQQYRLRSESLKTNVKAERQRLLPDISLGYFYGNNRFENAR
                     GYHGFQVGLGIPLYPGDQRSKIKANRIATDANELLLQNNLILLEMKYNNLKNELEKYN
                     KTIDFYNNTGKKLSDEITRTATRSYETGEIDFYQLVLSLENALKLTLDYYEAVSEYNR
                     IALEINYLHSPDS"
BASE COUNT        11434 a         8320 c         7960 g        11142 t
ORIGIN      
        1 taatggctga aaaatatggt aaatatccaa atgtgatata cgaaattttc aacgaacccg
       61 actattacac atggcccgaa gtaaaagcat attccgaaga actgataaaa accattcgcg
      121 ccatcgaccc cgacaatatc attctcgttg gttcacctca ctgggatcag gatattcacc
      181 tggtagccga agatccgatt gccggacaaa caaatctcat gtacaccatg catttctatg
      241 ccggaacaca caaacaatgg ctccgcgacc gtaccgatgc ggcaatggcc aaaggtattc
      301 cgatttttgt gtcggaatgt gccggaatgg aagctaccgg cgacggtccg gttgacgaag
      361 tggaatggaa agcttttctt gactggatgg aagcccgaaa aatcagttgg gtagtgtggt
      421 cggtttctga taaaaacgaa acctgttcca tgctgctgcc gcgcgcaaag tcaacaggag
      481 actggactga tgaacttata aaaccgtggg gaaaaattag tagggaggcc attcggaatg
      541 gtaatcttag aaaataagcc gttaaattaa aaaaaatatt cttcatccgt tttgaaaaaa
      601 aatctgttaa acaacaaaac atacatcaat gagaaataaa ttattagtat tgttaattct
      661 ttgtttcgtt gccggttcgg ctatcgctaa agtaacttta cccgatatcc tgagcgacaa
      721 tatggtgctg cagcaaaaca ccggtgtgaa actttgggga aaagccagtc caaacagtat
      781 ggtgaagatt cagccatcgt ggatgaaaat gactacaacc gtaaaaacag acaaaaaagg
      841 taactggata accagtattc aaacaccaca agcaacttat accccccaga cactcacttt
      901 ctctgatgga gaagtgctca cgctgaaaaa tattctgatt ggcgaggtat ggctggcatc
      961 gggtcagagc aacatggaaa tgcccctgaa cggattctgg aactgcccca tacaaggtgc
     1021 aaacgaaacc attgcatttt cgggacaaaa caaaggaatc cgatttgcaa ctgctcccaa
     1081 aacggctgct tacgaaccgc aggaaactgt aaaaggaaaa tggatggaaa gcaaccccga
     1141 aaacactccc tggtttagcg caacggcata tcattttgca caaacgatat acaagacgct
     1201 gaatgttccg gttggtatta ttgtatgcag ttggggtggt tcgagggttg aaagctggac
     1261 aaaccgggaa attcttgaaa cttacgccga catcgattta tcgaaaaaag gtattgaagc
     1321 agtggaagaa tggaaacgtc cgctggtgat gtacaacggt atgttgaaac cactcaccaa
     1381 cttcaccatt aaaggattta tctggtatca gggcgagtcc aacgtgggta aacacgaaga
     1441 ttatcccgtt cgttttgcaa aaatggttga actttggaga aaagaccggg gactgggaaa
     1501 tctgcctttt tactatgcag atattgctcc ctggatttat ggcgacggcg aatccggaat
     1561 ttcgggcgca ctgctgcgcg aagcacagtt caagtctcaa ttcatcattc cgaacagcgg
     1621 catgattaca actaacgacc tggtggagga atacgaaaaa accaacatcc acccgaaaaa
     1681 taaaaccgat gtgggaaaac gactggctta tctggcatta aatcagactt ataatttcaa
     1741 aggtatcgct gcacgcggac ctgaatacaa atcaatggag gtaaaagacg gtaggatcat
     1801 tttatcattc aactacgctc aggatggatt caaccgcgaa aacgacattc gtggatttga
     1861 aatttgcggc agcgacaagg tatttttacc tgcagaagcc aggcttgaca acaataatct
     1921 gaaaatcata ctttccaacg ggcagatacc cgagccggtt gctgcccggt atggcttcag
     1981 gaattttctg cccggaaatc tggccaacac ccgcgaactg cctgttgtac cgttcagaac
     2041 agataatttt taaatcatca aaaaataaat tgatattgca ttgcttttcg tgttaatttt
     2101 agattggttg gggagaaaac gttaataata aagcgttttc tccttttttt tgtattctaa
     2161 ttgtcgatgg ttcctatatt acaaacttct aatcaaaaaa gttgtacgta attcgaattt
     2221 gaatccactt aatgcattat tctttttaaa aaaaacaaca cagccaatat tttgtgttct
     2281 ggctgtgttt atgtttttta aaagaaaaag tatgactgat gttttatctt cttctgtttc
     2341 tccagggttt atcactttca gaaccggctg aataggcagt ttctctcttt ctcgatgaat
     2401 tatgttttcc gccttcatat ttccgttcgt ttctgaagga attacgtttt tcacctgatc
     2461 gcttatcccg cggattatct tttttgtctt catcatttga aaaactgcgg ctaccttttg
     2521 ctaccgatac atttaaatcc tgtgtctgtt caaaagctga taaaaccctg tctttctgat
     2581 cttcaaacac atcaaagaac gtaaatttgt tggtaatttc tatcccgccg atgttgatgg
     2641 atttatcatc ggtaatatcg ttgatgatac ccagaattct tcgcggattc attccttctt
     2701 tggttccaag attaatcttc aaccttacgc ggttacccga tttattacgc cggtttccgt
     2761 tttctttacc attgttctgt tccgttttgc catctctttc acgtggtgca tgttcggtga
     2821 tattgagatc gggagcgttt ttataataat cgaggaaacg gttgaactcc agcgaaacaa
     2881 atcgtttcag caattcctct ttcgaaaggt attccagctg attcattatc tgagccatgt
     2941 agggatctat ctgttcctca tttacatcca cgttttgcat tttatctatc tggtagaaaa
     3001 gttgcttttt acacacttcc agcccgttcg ggatctggcg ttgttcgaat ttccgtttga
     3061 gcatccgctc aatatccttg attttgaatt tctctttgga gtgaatgatc gaaactgaaa
     3121 ttccggtttt attcgctcgt ccggttcggc cgctgcggtg agtataaatt tccacatcgt
     3181 caggcaggtt gtagttgatt acgtgagtca gatcgctcac atcgagccca cgtgccgcta
     3241 catcggtggc taccagcaac tgcacattgc ggattcggaa tttctgcatc accgagtcgc
     3301 gctgaggctg tgataaatct ccgtgaagcg catcggcatt gtatccatcg tgcatcagtt
     3361 tttcggcaac ttccttggtt tcctgacggg tacggcaaaa tactattcca taaatatccg
     3421 gattcaaatc cacaatccgt ttcagcacca ggtatcgctg gcgagcctgt gatatgtagt
     3481 aaatatgttc aacgttttca gtgcctgaat tgcgcactcc gacagtaatt tcctgataat
     3541 catgcatata ttgctttgca atgcgctgaa tttcagcagg catggttgca gaaaagagca
     3601 gtgtgcgtct gttttcggga gtttctttca gaatggtttc gatatcatcc ttaaaaccca
     3661 tgttgagcat ctcgtcggct tcatcgaaaa ccaggaaatt gatgtttccg agttgaattt
     3721 ttccccgctc agtgaggtca atcaaccgtc cgggtgttgc cacaataatc tgtggtgtcc
     3781 ggtccagttc tttgagctgt ttacgtatat cttcaccgcc atacaccggc acaatgtgta
     3841 catcagtcat attggctgca tatcctttca tatcgtttga aatctggata cataactcgc
     3901 gcgtgggtgc aagtaccagc gcctgcacct cgcggcgttt tacatcaatc atgtttagta
     3961 tcggaagtcc gaatgcagca gttttaccgg ttccggtttg ggcaagcgcc actaaatcag
     4021 cttgttccgt aagcataaac ggaattacct gttcctgtac aggcataggt agttcatacc
     4081 ccagttgttc gacagccgaa agtatttcag ctctcaaatt gagttcttta aatgtcattt
     4141 attgtttttt attagtacac gttctgagcg gtaatccgaa gccccgtgaa agcaaaaatc
     4201 aaacgctttc agctattagt aagtataata agaaaagttc ccgggaggga aacgtgtacc
     4261 ttgaagatat ccaactgtct gattgacagc acaattcaga tttttaccca caacgcataa
     4321 gttgttttta aatcgggtgc aaagatacaa taaaatgtcc ggataataac attgattttt
     4381 cataaattta ctgctacaat ctcatcatat actgattttc acaaaaaacg acacaaaagt
     4441 cgtaaaaaat caggtactat tttctttcat tatattaaaa aaaggtgtat ttttgcatgg
     4501 aatttttaca aaattcaaac atttgattat caaaacatta aaaaaatatc tcgattatga
     4561 aaccaatgac caagagtccg ctgcagattg cccgttcaag ctatcagccc aaagtaccga
     4621 tttcactcac cggagacgta gcaatccatg agggcaaagt aacacaatca gttgcagatc
     4681 aggaagacat aaaaaaactg tttccaaata cctacgggat gcctctcatt gaatttgtaa
     4741 aaggtgaaaa aagtgattat ccgcctgtta atgtgggcgt tattctttcg ggcggacagg
     4801 ctcccggcgg acacaacgta atttgtggca ttttcgacgg cctgaaaaaa ctgaatcccg
     4861 agagcaaatt atacggtttt ctgggtggcc ccagcggttt ggttgatcat cagtacaccg
     4921 aactgactgc cgatgttatt gctgactacc gcaatacggg tggttttgac atcatcggat
     4981 cgggacgcac caagctggaa gaagaatggc agtttgagaa aggaaaggaa atttgcaaag
     5041 aactggatat caaagcgata gttattatcg gcggggacga ctcaaacacc aatgcctgtg
     5101 tactggctga atactacaaa caacatgatg caggaattca ggtgattggt tgcccgaaaa
     5161 cgattgacgg tgatctgaaa aacgagatga tcgaaacatc gtttggtttt gatacggcct
     5221 gtaaggttta ttccgaactg attggtaaca tccagcgcga cgccagttca gccaaaaaat
     5281 actggcactt tatccgtctg atgggtcgtt cggcttcaca tatcacactg gaatgtgcac
     5341 tgcaaagcca acccaacatt gcattgattt ctgaagaaat tgaagcgaaa aacatgacgt
     5401 taagagacat tgtggattac attgtagata taatcgtaat acgtgcaaaa tcgggtttga
     5461 actacggcac cgtacttatt cccgaaggat tgatcgaatt tattcctgcc atgaaaaagc
     5521 ttattgccga cctgaacgac atgctggccg ataacgacga tttcaatgcg atgacctctg
     5581 acgaagaacg acgtgagtat gtaaaaggag tgcttaatcc tgagagtacg gctgtttacc
     5641 ggagtttacc gaaaggaata gcccgtcagc tcaccctgga ccgtgatcca cacggaaatg
     5701 tgcaggtttc gctcattgaa accgaaaagc tgctaatcga aatggtagct aaaagactgg
     5761 ctcacctgaa agccgaagga aaatacaaag gaaaattctc tgcaatcaat cacttcttcg
     5821 gatacgaagg acgttgcgcc atcccatcca attttgatgc agattatacc tattctctcg
     5881 gagtggtagc ttcggtgctt atcaatgccg gtaaaaccgg atacatggcc tcggtgcgaa
     5941 acctcacagc tccggcatct gaatggatag caggtggagt gcctatcacc atgatgatga
     6001 acatggagcg ccgtcatggc aaaatgaaac cggttattca gaaagctctg gtacgtctgg
     6061 atggggaacc tttcaaattc ttcgaaaaat accgtgataa atggtcggac gaaacatcgg
     6121 acttcatcta tcccggtcct attcagtatt ttggcccttc tgaagtttgc gatcagccta
     6181 cccgaacctt actgctcgaa aaaggaaaat aatcattatt acattcagca caagttccat
     6241 ttagtttttg aattttaata tcctcccgat taccataagt gatcgggagt tttttttgga
     6301 tgaatcgtgt aaatagtaaa tgtagaaacg ttgtcgataa tccgtttttt tatcattccg
     6361 ctttctaccg gaatcagtat tttagggaga cacatttatt tagttactga taactgaaca
     6421 aaagcaacat aaaactgttt tttaattcaa aattacatcg aatcaaatta catgttctta
     6481 gtttgaaatt tgctgattaa tcaggaatca tttatccttc cggtcaaaat cttcaaataa
     6541 atttgttttt tttttgtaga tacttatttt tgaataactt tgtgaaaaaa attgaaaaac
     6601 gtttaaccaa gatgtataaa caagttttac gcagcaaact tctatacgtt tttacgttga
     6661 tgttagttgc aggaatatta agttcttgta tcacagcccg taaggtaaac tacctgcaaa
     6721 agccctcagg cgctatacct gcctacaaag gcgatatcgg atacgaagaa tacaacattt
     6781 ccggtgctga taaactctat atcagagtat attcacccga taaagatatt aatacgattt
     6841 tcaacggaag cagcagtcag atgatgatgg gcggcacaat gcaatccggc tcggattaca
     6901 ccgacttgta cacttatcag gtgaaaaata acggagccat aaaccttcct ttggtaggtg
     6961 atgtgtttct ggccggacaa actattcgcg aagcaaagaa aacagtggaa aacgccataa
     7021 aaaccaccat gaaagacgac tgttcggtgg atgtacggat tgtgggaaga tatttcagcg
     7081 tgattggaag cagaataaac gggaaatacc ccatcatgcg cgagaaaatg aatatctttc
     7141 aggccatggc catggcaggc gatatcggta ctttcggcga cagaagcaaa atcaaggtgt
     7201 tgcgcgaaac gcccaacgga ccgcagataa aagtgttcga cattcgtagc aaggatataa
     7261 taaattcaga gttttactac atccagccta acgatgttat ttatattcag gatgttaaat
     7321 ctcagttttt ctcagtaaca tctttcggct cggcattatc tacactcttt tccacatttt
     7381 cattcggaat gttgatctat tacctggcag tacccgaaaa accagctccc gtaactactt
     7441 ccaactgaaa ctaaaccggc ataagatatg tttacacatc aatatatgaa aacaaaatgg
     7501 attcatgcgt tactgattat tttagtggca atcggtttca gttcgtgcta cacacagagg
     7561 gataaaagct tgtggcagga ttcaaaatct ttccccaaat acgccaaagc agactacgag
     7621 caatataaaa ttcatgtaaa cgatgaacta attttcagag taacaagtac cgatgaggat
     7681 tttgtacgac ttatcgatgc tggttctacg gcttcacagc aacagggaat ttcatacagg
     7741 gtatatcagg atggcaccat tgatttgcct tttgttgact ctgtaaaagt agcaggcatg
     7801 actatcaatg atgccacgga agcaattcag agaagattca aacagataat tcccgatgct
     7861 gaagtaaaac ttgcactttc gaataaagtt tatactgtca ttggtgaagc cggcaacgga
     7921 attttcccga tttacagaga aaaaatgact atctatcagg cacttgcaca atcgggcgaa
     7981 atagcactca gcggcgaccg caaacatatc aggatattac gcgaaaaaac cggagaaact
     8041 gaaatcctgg agtttgacat ccgacccaag tcggtaatca attccaaata ctattacatc
     8101 tatccgaatg atgtgatcta tgttcaaaaa gaatcagcaa gtttttataa atcaaataat
     8161 tatacaacct tactcggact cattacttca tctatttcgc tctttaccac tgtattttat
     8221 tacacaaaag tcaataacaa ctaatcagtt tacctctaaa aacttaaccc tttctatatc
     8281 cccttaacat tatggaagaa aataaaaaca gcaatcaata cactcaattt tccgacaacg
     8341 aagaatctga tttcatactc aaagaatggc tgatgctatt cctgcactac tggtacgtgt
     8401 ttcttttctt tatcattctg gcgttaggag ctgcctacct caaaaaccga acctggattg
     8461 aatcttatca atccaaagga acgattatca ttgaggaaaa caggtacagt ggctctcaga
     8521 cattcatgca gggatttggc gttcaaccag gctaccgtaa tgtggacaat cagaccgtaa
     8581 ttctaacatc ttatgatctg attagtcggg tgattgacag catcccgttc ctttcggtgg
     8641 attacattac caaatccaga ttcaaaaccc ggaatttata caacgaaacc ccgattgtaa
     8701 taaatgccga ttttattgat ccaaaaattt acggtgtatt gttcaaactg agcatcaata
     8761 ccgacggaac ctacgtaata accgacgaaa acggttcgat tgacaagacc ctgaatgcca
     8821 aaggacgtct tggaattcca attcaacaca atttattctt tatcactgtt gagcctacaa
     8881 gcaacaagat actccgacgt gagatatttt tcagattccg tagcaaagaa tcgcttatca
     8941 gcgatttcat gtcgcgactc cagataggat ttattcagga tggttcatcg gttcttggca
     9001 tttcactcac cagcgaaact cccgaccgtg atatcgattt catcaataaa ctttgcagcg
     9061 tatttctgac cgaaaatctc gagcgaaaaa acgatgcagc ttcaaaaaca atggatttta
     9121 ttgaccagca actggataca gtttcaaaat cactggctat atctgaagga gccatgactc
     9181 agttcaggag aagcaatcag attgtcgacc taccaagcca ttcttccgag attctgggca
     9241 aagctactca gtacgacgaa aaacaatcgg cactcaacct gagagactcc tatttcaaat
     9301 acctgacaaa ctacctgaaa acgaacctcg atgcaggagc cgtggtagct ccaagcagcc
     9361 ttgggctgaa cgatccgatg ctcatgtcgc ttgtacagca gttcaacgac ctgattgcca
     9421 aaagaaacga aacgagcgac aaaaacccgc tttatgccaa atacaatcgc gaaattgaat
     9481 ctttgaaaaa ttctatcaac gaagtcatta caaacatgag ggcttcaatg gaaattgaag
     9541 gacaggattt gagccgtaaa atggccagcg tgcaaagcga tataagcgcc ttgccacgaa
     9601 aagaaatgca attgataggt attgaaagaa aataccgtgt ggacgacaat tattatacct
     9661 tctttctcca aaaacgcgca gaagcagcca ttcaaaaagc gtccaacagc ccggataaca
     9721 gcatactgga caaggcgaga atcgttgccc tgaccaacgg aaacaccaaa tccagaacaa
     9781 tcctgattta tctgctcatc ggatttttga ttccggcttt gtttgtagtg ctcaaagagc
     9841 tgctaaacaa cactatacgc gatacgaaag acgttgaaag aagcagtaaa tatctgctta
     9901 tcgggaccat caaacatact aaaaataccg atccgacact tacttccaag cgtccccgtt
     9961 cttcatttac cgaaatgttc agggtaattc gtacgaggat tgaatttatt gtacagcgga
    10021 aaaccaaaat tgccattttg gtaacttctg ccgaatccgg cgacggcaaa acctacttca
    10081 gcaccaacct ggcatcgact tacggtatga ccggattgaa aacactcctg atagatatgg
    10141 atatccgaaa accgagtatc aacgagcgta tgggactcga agaaacagac ggagttacga
    10201 actacctgat tggtgaaaaa aagctggatg aaattattct gaaaaaagaa gaactgaact
    10261 tcgatatttt gctcgcaggc agcgtaccac ctaatccggg tgaattaatc cgctcagata
    10321 agttgaaaaa catgtttctg gagctgcgcg aaaagtacga ttacatcatt gtggatacaa
    10381 gcccgattgg tttggtagcc gatgcttact cactggcgcc gatgatggat gcaaatttaa
    10441 ttgtagtgag aagtgaaaag accaacaaat cgttcttcag gaaactgaat gatcaactca
    10501 tatctgataa tctcaagcat ttctacatca ttctgaatga tatcaacatc gagaacactt
    10561 catacggcag atacggtaga aattacggtt acggatatgg gtacggatac ggatatgggt
    10621 acagcaaaga aaaagatgcc caaaagtatg tacagtacta cgaagacgat aaagatattt
    10681 gataagaaat aatggtttaa aaatagaaac cgaatagcct cagattgaat atttgaggct
    10741 atttttttca ccgaaccact acattgtaat tttgcagaat caatacctgc gggttatgaa
    10801 agtgaaaaca cctctgtaca gaatcgaaaa tctgacagag gtgaaatact taattcattt
    10861 tgtaaaaaag ccttacatag ttagcggaat gctttttact atgtcagctg tactcatttc
    10921 tctggctact tttgaatatt gctggctgaa acttcgctga gatttcatca gattacttga
    10981 ttgaagggca atgattttcg actcgttgat aagattcagg aacagaatac ttccgcgagt
    11041 agtggtttca ttttctttta cccttttaat ttgtcttttg gtgagtttta catacaagtc
    11101 agtcatcaga tcgcgcaatt gggtaatttg atcaaacccc gagtaatcgt tgctttcaag
    11161 catcaggcta aatttaccga agctatcggt caaggcattg tacatttgtt tcagatcatc
    11221 aatctgttct tttgaaaacg gtgtatggtt gttgtcgatg taggaatagc ttgattcagc
    11281 cattgctttc aatgatttgg tgatctcgaa tgaataatcc accagctgaa catattcctg
    11341 ctccaaatcg agcgcactta tctgaatggt ttcaatagtg ggtactactt catacatgcg
    11401 cttgtcttta tatttttcat acaaactgtt tgtttcagcc actacatttc tcatcgtttt
    11461 tctgttctca tttttcagtc cgtcaattgt taggctgaac gtattcagtg tagcctcaag
    11521 cacagttttc acttcgcttg tacattctgc cacaagttgt ttttctgagg ataactgcac
    11581 gtgtttgctc tcttcttttt cttttcgctt tgtatgcagt ttacccgatc tgaacaacaa
    11641 aaatcctaca agagcaagca tgacgaaaat ggcgattttt ccgccccaca tcagaaacaa
    11701 gccaaccagc aaagaagcac tcagtgctac aagcgctgtg agcagccatc ctgacaccac
    11761 tgtcaacaca ccgtttattc tgtaaacggc actctcgcgg ccccaggctc tgtcagcaag
    11821 cgaagttccc atggctacca tgaatgtaac gaatgtggtt gataaaggaa gtttcagcga
    11881 ggtacccaac gaaatgagga gcgctgaaat agtgaggtta acggatgcac gaatcaggtc
    11941 aaatgacgca ttgttctcaa tcgcaacagg ttcaaagcgt ttatctaaaa actctttcac
    12001 atttggtggc gtagatttgt caatagcttt gcttattttc atggctccgt gaactatgga
    12061 gcgcgaaagc gctgaagatg aaaatcgctc cactccttcc ccttttctgg ccagattaac
    12121 ctccgtttcg gtcacggtac gtgatttctt tgaaaaccac aaggcaagca ccatgataat
    12181 acctgcacca aacaaccatt gccattgcgc cgttacaggc ttgttcagtt cacccatata
    12241 cagcgtatca atagagccac ctgaggctac tacatcttta gctattgtaa acgaatccaa
    12301 accggccatg aaaaccccga taaaattaac aaggtcatta ccggcgaatg ccagtgccag
    12361 agcggctgtt ccggccagta caattatttt aaggatatta accctgaaaa caaactgaaa
    12421 aatggccatc aataccgtcc acccgagaaa tgcgtataat attgcttttc ccatgctgtt
    12481 attcagaaaa tcaagaaagg cttctgaaac taacacactt cctttcagac ctttaaatat
    12541 cgcaaagtaa gtgatggctg tcagtgcaat accgccccaa acggctccga gatatacgaa
    12601 ggatttttta taattgaacg aaaaaatcaa gcgtgaaata tacatgatga gtgatccgaa
    12661 gacaaatgca atagcaatgg aaataaatat tccgctgata atagccaatg cctttccact
    12721 gttgatatac tcacctaaac taccggaatc attggaccaa atggtaaaaa gcgatactgc
    12781 aagtgctgct ccaagcaact cgaagacgag cgatacggta gtggaagtgg gcaagccaaa
    12841 cgtattgaaa acatccagta aaacaatgtc agttaacatc acagcaagaa agagaatcat
    12901 agctgcgtgg aatgtcagtt tttcgggatg aaatactccg ctgcgggcaa cttccatcat
    12961 gccgctggag aaaagtgaac ctatcaacac tccaagagac gcaacggtga gtatcagcca
    13021 aagaggtgct actttggagc cgatacttga attcagaaag ttgacagcat cattggctac
    13081 tccgaccgct aaatccatca cagccaatcc gagaagaatg atgacaatta ttaaataaat
    13141 tggatccata ctacgattat tttatgtttt aagagttaac cctttttatc attacaaaaa
    13201 tatctgttta acattacaaa acggttacaa aatctgaatt acaaggagtt gaaagaaatt
    13261 gtttttaaaa aaaatcttac aaaaaaaaga atggctatta aaaaagagct actattgatt
    13321 gaaactcaac acggagatcc aaatatatga atgatgcctg aaacaaaaaa catgaatggc
    13381 tgtaagtctt ccgacttaca gccattcatg ttcaatcaga gagtttttta taattctcga
    13441 gcagttgctt cgctgcaatg aatgaacttt gttcattcct caacaccttg ttttcggcaa
    13501 tctgaaggag tttttgtatc gtttcattat tgtaaaaacc ggatttcaac ttctcgttga
    13561 tcgtttcgta catccagtat ttcgattgat ttgttctttt gctttcgaaa tacccattat
    13621 tctttacaaa tccgatgtaa tttattacca tttgccatac gtcagcaata ccggttttct
    13681 ctatggcgga gcagttgatt acttcggggc tccaaccgga ttcggccatc gggaataggt
    13741 gcagggcatt cttgaactgc tgccgggcaa gttttgattt ttccacgttt gtgccgtcgg
    13801 atttgttgat ggctattccg tcggccattt ccatgatacc ccgtttgatt ccctgcaact
    13861 cgtcgcctgt accggcaagc tgaatcagca gaaagaaatc gaccatggaa tgtactgccg
    13921 tttcgctttg ccccactcca accgtttcca caaatacggt atcaaaaccg gcggcttcac
    13981 agagcacaat cgtttcacgg gtttttcggg ctacaccgcc cagcgagccg gctgacggtg
    14041 aagggcgaat aaaggcgttt ttggcaacag aaagttcttc catgcgggtt ttatcgccca
    14101 gaatgcttcc tttggtacgt tcgctgctcg gatcaatggc aagtacggcc agtttatgcc
    14161 cggtttgcaa tatatgcatt ccaagtgcct cgataaaggt acttttcccg gctcccggaa
    14221 ctcctgtaat tcctatccgg attgattttc cggcatgtgg aaggcatttt tcaatcactt
    14281 cctgagctat ttcctgatcg tggggcagcg aactctcaac aagcgttaca gcctgactta
    14341 aaaccgacac atcaccagcc agtattccct ccagatattc accggctgac agtcgtcggc
    14401 gttttatccg ctttttgctg taaggattta ttacaggcac atctgtcact cccccgttta
    14461 cattcaaact tttgaattga ggatcgtttt caggatgttt gtgatgataa tgcattatat
    14521 ttacaaacgg aaaaattaaa tcactttgta gcttaccgta aatgttaaga atgtatttac
    14581 aggatcgttt gtctgaactg taaatgcttt tttgtattcc ccttttggca ggaactttga
    14641 atcaacatca attttgaaac tctcggtatg tccgcctttg atggttgcac gcaggggatg
    14701 aataataata tcgctgttat tattggtgat gcggcgaatt tcgagtggat tatttccggc
    14761 atttttaatg ttaactttac cgcgcacccg gtttcctttt ttgatattgc caatataaag
    14821 attacttgat ttcacttcca taatgggcga taaccgtttt tgttcggcgc tcattttgct
    14881 gaaatcttcg gtcacgtttg caaaaaccgt tattttgttt gcatctccgg ttattttttt
    14941 cccgttcagt gtcaatgtaa gtttatcatc tacgggtccc cactctgatg ctttggtgcc
    15001 gtcaaaagta aatgtaatgg caccctgctc gttaggcttc agggttttcg gagtgatgtt
    15061 ggcgctcagg tatgctggca actcgggcag cgttacagtg agattggatg gtgaagaatt
    15121 cagcatggca atggtacggg tttgagtaga tttactatct acattactca tttgcaccac
    15181 tttactgttc aattttaaag catcgatctg tatcggatag ttgtttgcct gaggtgcact
    15241 ctgagcagaa gatgatttag gttttacatc tcccttaata atcagactga ccatttcttc
    15301 ggtcgaatta ctgtaaacgt aaatttgctt gttgaatgcg cccggacgcc ccatcgggtt
    15361 ataggtagca gtgacgttgc ctttcttgcc cggttcaatc ggggtttttg tccattcggg
    15421 agtcgtacat ccgcaggatg cattcacacg ctgaataacc aacggggtgg ttccggtatt
    15481 ggtaaactca aaaacgtgtg aaacattacc accatcttcg gttatctgac caaaatcaaa
    15541 cgtcttttct ctaaaattaa tttcagcttt ttgggctgtt acacttgctg tgaaaaatac
    15601 tactgacaca gcaacaattg aaaataattt acgaatcata agaatataaa tttattatca
    15661 gtttatttta ttaaaaatac aaaatcatgc aaatttacga aaaatgtttt taagaacact
    15721 tccaaaatga tattatctgt ataaattaag gttaattgct gctaaaaaac catatctttg
    15781 cattcaattt cttttggaaa accactaatt catctaattc atgcttcctg ataaattcaa
    15841 ggctctttca gaagccgtgt ttccttatgt tgtagattgt taccgccatt tacacgcgca
    15901 tcccgaactt tctttcctgg aattcgaaac atcggatttc ataacaaatc aactgaaaag
    15961 catggatatt ccattccgcc agggtattgg aggcacagga gtgctgggag taattaccgg
    16021 taataatccg gctaaaaaaa caatcgccct gagagcagat atggatgctc tgcccatcag
    16081 cgaagaaacc ggacttgcct ggaaatcgct gaataacaac gtaatgcacg cctgcggaca
    16141 tgacgctcac atggcatgcc tgctgggtgc tgcaaaaatg ctgaacgaac tgaaaaacga
    16201 atttgagggg accgtattac tggtgtttca gcccggtgaa gaaaaagcgc ccggaggagc
    16261 cagcctgatg ctgaaagacg gtgtttttga tacactgaca cccgaactga tcatcggaca
    16321 gcatatttct gaagattacc ccacgggaac gctcgctttc agaagcggtg ttgtaatggc
    16381 ttcagccgat gaaattcacc tcactgtaaa gggacagggt ggacacggcg cgaaacctca
    16441 tctctgcaat gatactgtgc tggctgcggc ccatacgctg gtggcattac agcaggtgcg
    16501 aagccgcctt tgtccgccgc ttacacccat ggtgcttaca ttcggaaaac tgattgctaa
    16561 tggcgaaaca aacgtgatac ccggcgaagt aaaactttcg ggaacatttc gtacgtttga
    16621 tgaaaaatgg cgggaagaag cccgcggtca catcggtcgc atcattcacg acacgtgttc
    16681 cgcatttggc tgcgcagtag atattgaaat gcctgaagga tatccgtgtc ttattaacaa
    16741 cgaggaaatt aattgtaaat caaaacaata cgcatctgaa ttgctgggcg aaaacaacat
    16801 cctggaattt gatcaacgcc ttacttccga agatttcagt tttttctcac agcgttaccc
    16861 ggcgtgcttt tatcgatttg gaaccagagg tgaatccaat caacatatgg gtcggtctca
    16921 tacatcagga ttttgcatcg acgagaaatc gttgcttacc gggacgagcg gactggcctg
    16981 gatagcactt ggttttctga atggatagaa tttcgtttta gcagcaaaat gaatcagcct
    17041 atcccgtaca cctgcctgta gatttttgct ttaatttttt cgaaattctc agaagcaaat
    17101 tccaaatcat cgctcatcat ctccagtggt atataatctt cgcccacccg ataaggtggc
    17161 tgaagatatt caaacggtac aatttcaaat cccagacgcc ggtaaaattc tattctgcgg
    17221 gctccgaata ctgagttctc ggcttgctct acctctatca ccaacggttt agcagccaga
    17281 gagcgaacca gctttatcac tttctcgccc agtttatgcc cccgcagcgg aggttctatg
    17341 gcaatgtgct ccagaaacag aaaatcatca aaatcccaaa agtggaaaaa accaatcagt
    17401 tggttattca tcaatacggc attgcagttg aattgtttat tggtatcgag cagatgcctg
    17461 aaatcttctt ttgtatgacg ctgattttcg gaaaatgcac caaggtaaag ttccgtgagc
    17521 tcgtcagcat acttatcggc tgatgatagg attcgaataa agtccataag aggtattttt
    17581 gatgacaaaa atagcataaa aacaaaaaga acgcaataaa aaattacgtt ccttttcaat
    17641 ttcagtttga aaaaacttta tttcagctaa ttgacaatgt attcatcagc tgactgtttg
    17701 tcatattaac atccgcatcc gcatccgtga ctgtgattgt gatcgtcgtc gcatccgcaa
    17761 tcgtcgccac agccgtcgcc acagttgcca ccacagccac agccgcctcc gcctcccagc
    17821 agagcgttca gttcttcttc ggtggcttcg cgtacttcga gcaccttgcc tttgaagtgc
    17881 agattttcac ctgccagcgg atgattgagg tcaactgtaa catgtgtatc atttacagaa
    17941 actacctgag cctgcagccg gtttccttcg ctgtccataa gtggtactac gttaccttca
    18001 aataccattt cctcgtccag ctttccgtct atttcgaata cagcacgttc gagttctatc
    18061 actgcctcat cgtcataagg accgtatgct tcttcgtttg aaatggtaaa atcaaagctg
    18121 tcgcctgttt caagtccgtt caggttttgc tcaaatttcg gaagcatcat cccgataccg
    18181 taaataaaat tgagtggctg ctcagacgta gctctttcca tcaattcaag ttcgccgttt
    18241 tcgccgtcaa cgtaaagttc atattcggca tttactactg cattattcgt aattttcatt
    18301 cttttcttct tttttaaaat gtgtaatttt ttatataaat ttaatttcga ttctcaagcg
    18361 gcttcgcgga taaaaaaata ccgtagttac tataacttaa taccacacgg ttttgttctg
    18421 aatactttcg cgatttccct gtttttccca tttcaaatcc tgtaacatgc tggaattcac
    18481 tccaatccga aaattatagg ttttgtaagg accgaacggt acaaagtttc cggtaagcgt
    18541 ccagcaatgc agatttcggg tcacattgaa acttgtgtag gtaaactgtt tggcttcaaa
    18601 atcataggaa gttgtaccgc tgatttgcca accgggtgtc agctgaatat ttcccgaaaa
    18661 attaaggttg tgtgtcagcc ggagattgta ttccagtttt tcgtagtcaa acttgtctcc
    18721 atcgcccagt ctgaccgaat aattgaccga caaactccac ggaatagtta atttttcgta
    18781 gccgtcggca tccgcttcgg agttttccag attttgattg gtacccttgt tttgtacgtt
    18841 cagtgtgtga tcctggtttg gattgtttgg atcttcatcg taagcacctt tatcagattc
    18901 ggactgggcg ccatcagttt ttccaccttt tttttctttc cttttaaaag tttcattgga
    18961 gaaagtatag ctatatgata aactcgttcc catgaacttc ggaaattttc cattattcca
    19021 gcgcaactgg ttgattcggc ggactgattt tccgtcgggt cccacgccat acatatatgg
    19081 atcaaatgca ccactaagac tcagagtata agctttaccg agtttaatac gcagattggc
    19141 gctgaaattt gaccattgca tggagtcggc agccagatta tagccgctgc ttatggcaaa
    19201 ctgatcaatc agacttacaa ttttagtagg ctttgtatcg gtagtgtctt tgtcgttacg
    19261 gagtttcatt tccaggttat ttgaaagtga aaaaaacaca ttcccgtttt ttcccctgcc
    19321 cggaccgcca tacatcgatg tttcgaacct ggaataatga acttcttcca tcagttttcg
    19381 ggtggggtcc ttcggatcgg cgtatgtttt catatatgta tcccagaacc cccatctcgg
    19441 atcgccaaaa tcgggagtat atcccattcc cacgctcgga tcgagtttat gacgaatggc
    19501 gattacctta tctccaaaca atttccggct gggagtgtag ataccgtaga gcgtcgtatt
    19561 agcagataca ttcatattga agtcatacgc tcttttgaat ccgttcactc ttgtatcaac
    19621 ggttcgctgc tttgcttcgt cccatgctct ttcaatcgaa tacatatacc acctttcgcg
    19681 ataactgaat gatggcgaca ctgttatgta atctaacaaa ttaaaagatg ccgatatcgg
    19741 aatagaatgc tgcattccgt tgcgccagtc gtttgcaagg gatgattgca gaattttatt
    19801 ttctttggcc gtgatgctgt tggatatggt accggaatac gacatggata ttttttcgta
    19861 ccaacgctcc ttccctattc tgtttttctt cttgagggga taaattctgc tcatcgaaat
    19921 agagatgttg ggcaaggtca ggcttatcgt ggagtcgcgg gttctttgat caatccccag
    19981 gttggcagta aacgaaaatg gattgttcgg aaaccgctgc gagaaagata cgcttgagct
    20041 tttggtgttt tcggcattca caggtgtata gtaactgttg atgttgctcc tgttgtagcc
    20101 acttgtggag aagttaacgc ttgccgagaa agtccggaaa ggatttactt tgggattctg
    20161 ggagtgcgac cagcgaatgc tgaagttttt ggatttattg taatcgggca gatctttttc
    20221 accggtcaca tcttcgcggt aagtaatgct cactccgccg ttgtatttat atcttttcag
    20281 gtagttagtc tgtgccacca cgccccaggt gcctttcagg taaatatcac ccttgatgct
    20341 taaatccata taatcgttga tggcaaaata atatcctccg ttggtcagac cgaatccgcg
    20401 tgaaaattca gtatcgaacg agggcatttc aatacccgat gaatagtttg tattaaacgg
    20461 gaaaaaaccg aaaggtaaag ctatcggaac aggaatatca aggataacca ggtttgccgg
    20521 tccggtaact acgtattcac cgggcttcac cttggctttg gaaagtccaa ggtagaagtg
    20581 cgggtgatca tgcaaatcac aggtagtgta ttttccctgg gcaatgttaa gtatatcatt
    20641 gtcaataatt tttgttttat tgctgataat aaatccatct ccctgctggg ttacggcctg
    20701 ttttacatac cctcttttga gtttgaaatt gtatgatatt tcgttggagg tatattcggc
    20761 atcttcatcc ttgaaaatcg gcttaccaga aagtgttccg gtgctatcca ccgtactgta
    20821 ggcataaatc atcgagctat ccatccggat gcgaaccaga tttgcctctt tcagattcat
    20881 ttctttgtat ttcacatcgg tacctccata cagaaatccg gtgccgttac cgaaaaatac
    20941 gattgaatcg gaagccgaat aggtaatttc agcatcaaac tgttcggctt tctttttggt
    21001 ggtgtcattg ggtgccggtt ttttcagcgt atctgccggc tgattaactg ataaggggac
    21061 ggggacagga tttgtgttag tttgttgtcc gtttgcatat tgcaacaacg agcaacaagt
    21121 taacaaacag actaatacgt ttactcttat gttatgtaag tttttaatgc gatgcatcgt
    21181 atagtttata tcccacttgt tggaaaatgg agtgcaaatt taagcaaaaa ataagtctga
    21241 aaactaattt gatgtcaaat ttataattaa tttgatattt ttgaatttat taataaggac
    21301 ttcaatcaat taatttgcta cttttgcaca atatttgatt aattaatgaa taaatcccaa
    21361 aatcaggcat tttgaatcag atgaaatcac tttataaaat cacaggagcc ataatcatgt
    21421 taatgattat ttctactgct tttttattcg ctcagaagcc ttttactgtc gttatcgacg
    21481 ccggacacgg ggggcacgac tccggagccg tgggaaaata ttccaaagaa aagaacataa
    21541 acctgtcggt cgctctcagg ttgggcagca tgattaaaga aaactatccg gaaattaaaa
    21601 tagtgtatac acgcaccaca gatgttttcc tgacacttca agagcgtgcc gacatcgtga
    21661 acgataacaa agctgatatc tttttctgca ttcacaccaa tgctaccggc ggatcgtcag
    21721 cccacggcac cgaaacgtat gtactcggac ttcacaagac aaaatcaaat ctggaagtag
    21781 ccatgagtga aaactcagtg atgatgctcg aagacgacta cgaaaccagg tatcagggat
    21841 ttgacccgaa ttcagtggat tcttacatca tttttgaatt catgcaggac aaatacctcg
    21901 atcagagtct gagaattgca tcgctgattg aaaatcagtt cagacacgct aaccgctaca
    21961 gtcggggtgt gcgtcaggcc ggattctggg tgcttcataa atctgccgca cccagtgtgc
    22021 tggtagaact gggatttatt tcgaatgctg acgaagaaaa atttttgaat accgacgaag
    22081 gtaaacacca gcttacaaaa gctatctata atgcatttga ggcttacaaa cgcgatttcg
    22141 ataaaaaaac aggcatcagt gtaactccga aagaaatgac ggtatataaa gccgacctga
    22201 aaaaagacag tgttgacgaa aatcaggtaa tatataaaat tcagctcttc gccttagaga
    22261 ataaaataaa tccgaacggc agggaattca aaaaactgaa cgatgtaaat tactaccgag
    22321 agggtaaatt ctacaaatat acctatggtg aagaaaccac atttgaaaag atagaggcat
    22381 tgagaaaaag cattgctgac aaattccctt acgcttacgt aatagcattc agaggcaacc
    22441 agaaaataac ggtagaagaa gctctgcgaa gaaagtaatt cgtcagcaat cacattctca
    22501 ataagattca gataaaaaac aacaacatga gaaaaaaata tacccgcgaa gtaaagattg
    22561 gattaatggt gatcgtagct atattcatac tttacttcgg actgaatttc ctgaaaggaa
    22621 ttgatatatt tcaaaaacaa tcaagctatt acggaaattt tgaaaatgtt gacggacttg
    22681 taaaatcatc gtcggtaaaa gtgaaaggct tcaaggttgg tcaggtttcg gatattcagt
    22741 acgatttttc gaaaaaagaa tcattcaagg tcgtaattac tgtttcggaa gacataaaac
    22801 tacccgtagg aactaccatg cttctgaccg acgaaggtct ccttggcgga aaatttattg
    22861 aactggtgta cagccccgtg tccgataagc agtttaccca ccataagaac gacaaactcc
    22921 ccacgcagac aagttccgga ctgatggcgg gtttaaccgg cagcctgatg ccgaaaattg
    22981 aacaggtaat acagcaggca gattcgttaa tacggtcggt gcggacgctc tcagaaagta
    23041 cccaaatcac gaactcactc aactcactcg aaaatactac agccgatctg gccatcactt
    23101 cagcccaact gaaaggattg atgagtaatc agatgcctgc aatgatgaat aatattaatg
    23161 ccgtaacagg cgacatcaga tttgtgagtg agaacctgaa aaaagtagac tttgcaggta
    23221 ccatggaaaa tgtggatttc accataaaaa acctgaatga ttttacccgg aaactgaaca
    23281 gcaatgaaaa cagcctgggc atgctcatca acgataagaa cctgtatatc aacctgaata
    23341 acactgctga gagtgccgac aaactgctta ttgacctcaa agaaaatccg aagcgctacg
    23401 tacatttttc acttgttggg aaaaaataac aaatgttttt atttttagtc attctaaata
    23461 acatttcttt tcaaattatt ttttcataca gttgaaacat ctgttaaaca acgatgtcga
    23521 cgttttttga gtaaaattcc cgattttaac atcaaattgt taatgcgctt aaaatcagtg
    23581 atattcgaca aatatattct gttaaacatt tgactatcaa atacctatca gcacccactg
    23641 gtattcaatg atttatgcag attttgtaat ccaaaaaaaa aaagacattt ttttatttga
    23701 aaaaagttat cgaattttgt aagtgagata tcgcccttca ttatctccaa tattttcaat
    23761 caaagtcgct cggcctcggt cgagtttttt atccgctaat ttttaaaaat atatttgaat
    23821 tacattatga gtcgtcacga gcagttgtgg aatagttgtc tgaaaattat taaggataat
    23881 attaatgaag catcctttaa tacatggttt tcgcctattg ttcctgtcag gtacgaaaat
    23941 aaggtatttg tacttcaagt tcccagccag ttttttgtgg agtacattga agaaaaatac
    24001 atcgatctgc tcagaatgac tcttacgcgt gtagctggca gcggcactaa actcgaatac
    24061 cgcgtactga ttgataaaag cagcggcaca ggaactcaaa taccgagtac agccgaacaa
    24121 aaacccaaac aatttggatc caactctgca cagagcacat tcgtatcacc ataccagcgc
    24181 ttacaactgc cggaactgga tcctcagctg aatccttcat tcaatttcaa cagtctgata
    24241 gaaggaaaaa gcaataaact ggcgcgtact gccggactaa gcatcagcaa cgatccggga
    24301 aaaacaattt tcaatccgct tttcgtatat ggaaaatcgg gtgtaggcaa aactcatctt
    24361 gtcaatgcca ttggagtaat gaccaaacaa ctgcatcctg aaaaacgggt actttatgtt
    24421 tcggccaata catttcagat acaatatacc gatgcggtac gaaacaacac acaaaacgac
    24481 tttctgaact tctatcagac aattgatgta ctattaattg acgatataca ggagttttca
    24541 ggtaaaacgg gtactcaaaa cactttcttt catattttca accacctgca ccaaacgggt
    24601 aaacagctgg ttcttaccgc cgaccgttcg ccactcgaaa tggtaggcat tgagcagcga
    24661 ctccttaccc gtttcaaatg ggggctttcg gctgaaattg aaaaaccgga ttttgagctt
    24721 cgcaaatcca tcctgaaaag taaaatctac cgcgacggac tggaaatacc cgaaaatgtg
    24781 gttgatttta ttgccgaaaa tgtggttgac aatgtacgcg acctggaagg tgttctcatt
    24841 tcgctgctgg cgcattccac acttaccaat atgcctattg acatcattct ggcagaaagg
    24901 gttatcagcc ggattgtgag cattaccccg aaagtaatgt ccattgaatt cattcgcgat
    24961 acggtgtgcg atttcttcaa actctctgta gacgctattt ctaccaaatc tcgcaaactg
    25021 gaggttgtgc aggcacgtca gatagccatg tatctttcca aaaatctgac taaaaactca
    25081 ctttccacca tcggaaatta cattggtcag cgcgatcatg ccaccgtact tcatgcctgc
    25141 aaaaaggtaa tggatttgat ggataccgat aaacacttca aaaagaacgt agaagaaata
    25201 gaagaaaggc tgaaaaaata aaattcctgc tttaaaaaat taaaaaacaa tatctgggat
    25261 gtataaatca acatctcaga ttttttttag gcaaatcttg ctgatttttt tccttactat
    25321 caaaaaaaaa cagtaatttt acatcgaaaa caataaccaa cgaaaaatca tgattttcaa
    25381 atttaccatc ctgtccgacg aagtggaaga ctttgtcaga atcatcgaga ttgactcaga
    25441 atcatcattt ctcgacttgc aaaatgcaat tctggattcc gttgattacg aaacaaatca
    25501 aatgacttca tttttccttt gtaacgacga ctgggaaaaa gaacaggaaa tcacattggt
    25561 tgaaatggag tcgagttcgg aatacgataa cctggtaatg gaagatacgc tgctggacga
    25621 aatgcttacc gacgaaaaac agaaactgat gtttgttttc gatataatat ccgaaagggc
    25681 atttttcatc gtactcactg aaatgatccc gggtaaagac ctggacgaag cagtatgcac
    25741 aacttctacg ggcaatcctc ccgttcagat atcggaagat ttcgatatga atatcgctcc
    25801 gggaaaaact tatcagattc attccgacga gaatttctat ggcgacgagg agtttgatct
    25861 cgacgaactg gatgaagaag gatttggcga tttgaacttc gatgacagca ctttgtttga
    25921 tgatccgaaa gcataattta ttaatataca aataattgaa cagaaaacat ttcagttgtg
    25981 aaatgttttc tgttttgttt tatcccaaaa attggctact ttaatcgtaa tacttggtcc
    26041 gaccggagtc ggtaaaacga acatcagcct ccggctggct gagaaattcg cttgcccgat
    26101 cgtttcgtca gattcaaggc aattttaccg cgaactgaaa attggcactg ctgcaccctc
    26161 cgaagatgaa ctgaaacgtg taaagcacta cttcatcggc acccattcgg tatttgacga
    26221 gtacaacgcc gggcagtacg aacttgatgc cgtcagtctg atggacgaat tattcctgaa
    26281 acacgatttt gtacttctaa caggcggttc gatgatgtat atagatgcca tttgcaaggg
    26341 atttgacgat attccttcgg tgaagcccga aatccgtgaa ttctggcaaa aggaatatgc
    26401 cgcaaaagga ctggagcata tccaaaacga gttgaaaaga ctggatgaaa aacattacca
    26461 tgaagtagac ctgaagaatc cgaaacgaat tatgcatgca cttgaaattt gcagcatgac
    26521 cggaagacct tattcggagt tgagaacagg aggtaagaaa atgcgaaatt tcagaatatt
    26581 gaaaattgga ttaacacgtc cacgcgccga actctacgaa cgcattaata aacgggtgga
    26641 tcaaatggtg gaacaaggtc tggttgaaga agcccaccgg ttttacccgc acaagcacat
    26701 gaatacactc aacacagtgg gttataaaga actttttgca cactgggacg gcgaatatga
    26761 cctggaacat gccataaacc tgataaaaca ggatagcaga cgatacgcaa aacgacagat
    26821 gacctggttc aaccgcgata ccgaaatcaa ctggtttcat ccggatgaag aagaaaaaat
    26881 cattcatttt attacctcca acacctctgg taaataatta ataactgatg aaaatactcg
    26941 aaaacacttc actcaagtcg tataatacat ttggaataga tgttaaagcc cgttttctgg
    27001 ccgaatataa ttcggtagat gaactgaaag agttgctgca atcggaaact gccagacaaa
    27061 accgcctgct gcacatcgga ggaggaagca acctgttgtt tctcaacgat ttcgagggaa
    27121 ttgtgctaca ttctgaaatc aggacaatcg aaacctgtga agaggatgcc gaatcggtac
    27181 ttttacgcgt cggctcgggt gtggtgtggg ataattttgt ggagtattgt gttgataaaa
    27241 attattacgg cgttgaaaat ctgtcgctta ttcccggtga agtaggcgct tcggctgttc
    27301 agaacatcgg tgcatacggc atggaagtaa aagacacgat agaaaaagtt gaattcattg
    27361 aaccagagag tctcaattca cggattttta ccaacaaggc atgcaattac ggctatcgga
    27421 acagtatttt caagggagaa ctgaaaggaa aagcaatcat tactcacgta tatttccggc
    27481 tgaagaaaca ggtttgtttt gtgctggact atgccaatct gaaagaatct gtggaacgaa
    27541 acggtgaaat caatctccga aacatcagac aaacggtgat tgaaatccgc gaaaagaaac
    27601 tcccgaatcc aacattactg ggtaatgccg gcagtttttt tatgaatccg gtcatcccta
    27661 aaaatcagtt tgaaatgctg ctcctcaact accccaccat gccacattat ccggtttcgg
    27721 atacagcagt gaaggtgcct gccggttggc tcatcgaaca gtgtgatctg aaaggaaaga
    27781 aatttgaccg tgcaggcatt tacgaaaaac aggcgcttgt gattgtgaat ctgggtaatg
    27841 caacaggcca cgatatagct actgtagcct cactcgttca gatttcggta aaaaaacggt
    27901 ttggcataga tatcattccc gaagtaaatt atatctcgta aaaaaagttt ttcagtacag
    27961 ccactggtca ttttcgaatg aaatacaccc ggaatgacac gctttggtcg tcagtaaagt
    28021 tttccgtttg tttcccagat tttgtagttt acttcaaatc ccgaaggagt gtacaccact
    28081 attggcaaac gtgaattgta agccatcagt tttttttcac tgcttacaaa tttttctgac
    28141 attatcttgt ctctgcagtt cattttggtt gaactgtatg tcccgtcgga ctgaaaaatc
    28201 cagtaatcaa aaccaaaccc ttccacttgt ccggattcaa aggttccatt caacctgtga
    28261 tcgttacagt ccactttcct taataaacct ggtataatct ccactttccg gtttatttca
    28321 tcggcggctt caagctccgg caggtaaagt atgtgtttta cgtaaccggc tttttcgggg
    28381 aagtaattcg tattctgcag ggcttcttcg gatgcaagtg tgccatttcc gttgagtgca
    28441 actgattttt cgtcgccggc tttccacaag cagtacttca cctgcagata gcgtgaaata
    28501 tatactacca atggcacatc gctgcggtaa tcggtaatga ccgattcgcc ggttacaaat
    28561 gcaggtactt tggattcgtc ggggcaggcc attttagtag aatagatttc tccgttcgta
    28621 ttgaagagga agtatgaatg accattctga aatgtcttgc tgagcatttg tccctgcaaa
    28681 ccgtaccggt tacagtctgt caacaattca cgacccggaa aaatctcgat tttctgttgc
    28741 tgttcctgtt cgggagttaa tttttcgaga aaaatagccc gcctttcaaa tcctgtgatt
    28801 tcaggatatc cggtcattat tttttgcatt tcgggtgtaa atgcttcttt tccggtactg
    28861 acctgccggg tggtattaca ggcagaaagg atgatcagag gcatcagggc aattaaaaag
    28921 aatttcagtt tcattgtcag atttttttat gaattgttca ttaaaaacaa aagtcatgca
    28981 aagaatctaa cttcctgaaa atttcttcat ttttatcatt ctttagtttt ttcggtaaaa
    29041 aaattagcgg tacaactccg gaataaaacc ctgctgcaaa gttgcggcga gaatctgggc
    29101 aatttctctg cgtgtgagtc cgagtagact ttcacccact tcaaagagaa aattgatttc
    29161 cttattgaaa atgctgttgt cagacatagc gatgttggcc atgtactgaa gcagctggaa
    29221 tctttcggcc ggattttgct gaataatatt agatatagac gatttaaaca cctcttcggt
    29281 gcttgtgttg catatttcgc tgaggtattc atgcggaaaa accgtgtaat tgctcaacga
    29341 ttcgaggatg ctttcatatt cgtcgtcgtt gatatttttg tcaatgttgg ccataatcag
    29401 ccctgcagat gccagaaact gctttctgaa agcatccatg ggcgatgtgc tgatggtttg
    29461 aagcatggca atcagtttgt ccatttcggc attgagtacc tgatcggtgt tgaattcctt
    29521 gtcgtttttg aaatctccat aaagtgagga ctccgaaaac aactttattg ctttgattct
    29581 cagcggattg accggatgag taaacatatt cctgttctgc gtgaaatatt ccagaatttt
    29641 ctcattatca atcagatatg ctttgtaatc aaaatcaatg cggtcggtac ttaaccctga
    29701 ggccattttg aaaaaacctg aaacacattt ttccacgttg gggctggcta taaatccaaa
    29761 acgatctgcg gtaagttcag aaactttctg ccaaagttct attctgtgag ccagaatgag
    29821 cggcactttt tcgggatccg gaaaaatgaa gttgatgagc cgcactaccc gtgcatagcg
    29881 agtaatcaaa tggccgattt catgacccac tacaaaccgg agttcatcat cgtcgaacat
    29941 gctgataagc gctgaattga tgttgatgat gtcggtatct ttatcatcag accgtgtgag
    30001 ggcaaatgca ttcagttcgg cagaattggt gatgtaaaaa tcgatgtttt ttttataatt
    30061 cagtttgttt tttacatcgt gaaaaatgtg atacaatttc ggcgacattt tttcagttac
    30121 cttgaaactg tgaccttcca gaatattttt ccagatattt tcttccggtt cgtaatttgc
    30181 ttctgcaatg attttttcca ccagtttacc ctcaagtgtc tgaaagagat tgctctcaag
    30241 ttctatttca agatccacac gaatgtcatt atttttcata ttcggttatt tttttttgag
    30301 atttagagtt caaagataaa gaaaaataca aattacgcca ttgaatgtat ttaaaatatc
    30361 gaccaaatca gcagattaca acccaaaatt cattaaaaaa aacattattc gtacttccaa
    30421 agcgaacttt ggtaaaaaaa acttactttt gtttttcaaa attcctgatg agttaagtgc
    30481 atgttgtcaa ttcgtcagca tcaatttttc aaagaactat taatttattc aatttatgaa
    30541 agtaaaattt attggcgcgg ctcgcgaggt taccggaagt aaaacactga ttaccaccga
    30601 tgatggtaag aaaatattgc tcgattgcgg catgtttcag ggaaaaggac ttgagaccga
    30661 cgagctgaac cgacgaacag gtgtgaatcc tgccgaactg gatcatatca tcctctcaca
    30721 tgcccatatc gatcattcgg gattaatccc ctatttttat aaactcggct tcaggggttc
    30781 ggtgatttgc accaccgcca cccgaaatct ttgctccatt atgctgccgg acagcgggca
    30841 catccagcaa gctgataccg aatggttcaa caaaaaaaga gccagacaaa acctgcctcg
    30901 tgtggagccg atttatgacc tggaagatgc tgagcaggca ctcaaactgt ttattgctgt
    30961 ggataacaac cgccgtttct acatcaacga caaaatcaac gtaaaattta ccaataccgg
    31021 tcacatgctg ggaagcagcg tggtgaatct ggaaattaca gaaaacggaa aaaaaatacg
    31081 attggcttac acgggcgaca tcggaaggcc acacaaccgc attctgagcg ggcctgtgcc
    31141 tttcccgcaa tgcgattacc tcatcaccga gtccacctac ggcaaccgac tccatgaaac
    31201 cgaaaaacaa accgaagaag acttccttaa aataatcacc gagacctgcg ttgtgaacaa
    31261 gggtaaactg attattccct catttgccat cggacgcact caggaagtgg tttttgtact
    31321 caataagttt ttcaacgacg gtttgctgcc ccggataaaa gtgtatgtgg acagtccgct
    31381 tgcagtcaat gccactgata tattcagaat gcatctggac ctgatgaacg aagacgtaaa
    31441 acgcgaaata aaggaaggcg atcacgatcc gtttggtttc aataacctca tttacatcaa
    31501 gaaggtggaa cagtcaaaag agctcaatga actcaacgaa ccttgcatta tcatttcagc
    31561 ctccggcatg gctgaagcag gccgcatcaa gcaccacatc gccaataaca tctccaatcc
    31621 gcacaataca atcatgctgg taggctactg ctctcccact tcgcttggag cacgtattca
    31681 ggaaccggat ctgaaagaaa tatctatttt cggaaaaatg cacgatgtga aagcccggat
    31741 aacgagaatg gaggcatttt ccggacacgg cgactacaac gagatgaaga gtttcctcga
    31801 atgtcagaaa aaatctgcgg tgaaaaaaac atttctggtc cacggtgaat acgaagttca
    31861 gcaattctac cgagaaatcc tgtcaaaaga tggttttaag aatattgaaa tacccgctta
    31921 cgggaatgaa tttgagttaa atcaggtgaa ttagctccct aaatctccaa aaagggactt
    31981 cagagtgagt gaatagttga attggaaact tcatatttaa aacttctata gttatgttca
    32041 aaaaactgat attaccaatc atgttttgca tggcaacagc cggtttttcg caggaaaact
    32101 ataacatgct tgttggaacc tatacacgtg gcactgtaag caagggaata tattcgctga
    32161 cattctccgg caaaggcaaa tttatttccc aaaaactgtt ggctgagagc gataatccga
    32221 gttatctggc attttcgccc gaccggaaat tcgtgtatgc agtgaacgaa cacggcgaag
    32281 aaagcaccat cagtgcattt gcctttgata aggcaaacca aacgctgact ttcatcaaca
    32341 aagttccggc agcaggagcc gatccgtgct atatcacagt atccgacaag cacgttttca
    32401 cggccaacta ttccggtgga agtatttctg tattccacag gttgccgaat ggtaagctca
    32461 gcgaagcctc acaggtaatc acacatccga ggaaaaactt cggaaaccgg cgttacgggc
    32521 cgagcaatgc ccatcagatt attttttcgc ccgacggaaa gtacctgatg gctaccaacc
    32581 tgggaactga ccgggtgttt acctacaaat acaaccccga aagtgacgac gaggtattga
    32641 gttatgtgga tgaaaccgga gtgaagcgcg caagcggacc acgccacctc gcattcagca
    32701 ggaatggaaa attcctttac ctggtgcagg aactcaatgc aggcatcacc gtattttcag
    32761 tttcggacga tggaaaactg agtgtaattc aggaaacaac gctggtaacc gacagcacga
    32821 aaaaaaacgg tgctgctgat attcacctca gccccgacgg aaaattcctc tatgccacca
    32881 atcgtggcga agccaatacg atcacttgtt ttttgataaa aaatgacgga aaactgcgtt
    32941 tcgttgaaga atattccact tttggtaatg ctccccgaaa ttttaccatc accgttgacg
    33001 gcaaatacct tttcgtcggc aatcagaaaa ccaataacat caccgtttac atccggaaca
    33061 gatgcagcgg taaactgaaa ctgcttgacg ataagacaga actgggagca ccggtttgtc
    33121 tggtgttgta ctaacacatc ccatgatctg caaaaatccc cgaaacctca agctttcggg
    33181 gatttttgca gattgagtaa agattaaatt cagagagttt cgactccact cagtacaatt
    33241 tctttttcgg caggaagtcc gctcaaaatc tcgataaaat ttttattact cttaccggtt
    33301 tccacttttg tttttttcag gaaaaactta tctccctctt ttttatccag cacataaatg
    33361 aaatattcct tttcctgctt ttgcactgcg ctttcaggta cagcctttgc atctttgtta
    33421 tttacagtaa taatggcttc cacatagctc tgatttatta attttgattt atccgtttcg
    33481 tctatccggg ctatacaatc aatcgtttta gaatcgggat tgatggtttt tcccaaggca
    33541 atcagtgttg ccctgaggtt ttgactgctg tttccgctta tgccaaaccg aattcgctgc
    33601 cctgcccgga gtttatgaac atccgcctca aaaactgaca gttggagctg cagactggtt
    33661 acattcacaa tttcagcaat ttcgttttgc atttccacgt actgaccgat gacagcgccg
    33721 gtttttgtaa taaatccgct gataggtgat gtcaccggaa aagaagtgta catttgtccg
    33781 ttttctattt gcgacggatt gagccgaagg gccagaatgc gtgttttcaa cgcattgtag
    33841 ctcgccagcg acgctttata taaactttcg gaagcggtaa aatctttttc agctcctatt
    33901 ttatcagccc gcaatgcttt caaacgctca taatccagtt ttgctttctg aaaaccagcc
    33961 gaggattcgg caaactgctg ctgcaaactg agaaattcgc ttcctgaaac actgcatacc
    34021 acctgtccct gacgcacaaa atcaccggtt ttgaagcgaa tattttctac agtgccggca
    34081 atgggtgtac ttactttggc aattccgttg acgggtgcaa acaaaaatcc cctgcaagct
    34141 acctcgtcac tgaaagtctg caaggttgct tcacccagtt gcattcggtt tgtgttgaac
    34201 tgttcggtac ttatttcaat cagatcagca tccgcatttt cggacaattc attgccgttc
    34261 tccgtttttt ttccgcagga aagaaaaaac aacaggataa tagaaaaaat aaataaatgt
    34321 ttgaatgttt tcatagactc tttcttaatc cttaatcgga attattaaag atgattttat
    34381 aatgtactct atccggtact cctttccatt aagaatcggg tgaatgtaaa taattgattt
    34441 caagtgcaat tcgattatat tccgaaacgg cttcgtagta atcaagcgtg agtttaagtg
    34501 cattttcaag gctgagtact aactgataaa aatcaatttc accggtttcg taacttcgtg
    34561 ttgccgtccg ggtaatttca tcgctaagtt tcttacctgt attgttgtag aaatcaatgg
    34621 ttttgttata tttttccaac tcattcttca ggttgttata tttcatttcg aggagaatca
    34681 ggttattttg caggagcagt tcgttggcat cggtagcaat tcggtttgct tttattttag
    34741 agcgctggtc gcccgggtaa agcggaatgc ccagtcctac ctgaaatccg tggtatccgc
    34801 gtgcattttc gaatcggttg tttccgtaga aatatccgag tgaaatatcg ggaagcaggc
    34861 gttgtctttc ggcttttaca tttgttttta gagattccga tcgaaggcgg tactgttgaa
    34921 tttcaggact tgtctgcata tcgggcttac ttacggtgat gattttcatc ggtatctctt
    34981 ctatgcgaaa atcctctccg gtttgcatga ccgatttcaa accagcataa gcatgttgta
    35041 tctcgatggc aatttttgaa atttccagcg cagattgctg ttttttggcc tgcaaattga
    35101 gtttgtccag ttcactgata tcgcccaacc ggagctgagt atctgccatc gaattcagcc
    35161 ggtcgtacat actgtcaatc cggttgaaat aacgttgttt ttttagcaaa aactgatacg
    35221 aataataaga agatgccacc ttttttttca ggatgagttg ttgtttttga agttccagtt
    35281 gggcttgtgt aacctccatc cggttcattt tattattcag gctgtgtatg gttggaaaca
    35341 gaaacgtctg ctccactccc aatgtacgca gcggatgtcc gttttctgcc acattagcct
    35401 gatcgtatcc gtagtaaaag gtggttttat ccagttccat cacccggctt gccaatgctt
    35461 ttcgttcatc aatcttcaga aggtaagctc ccagtgctct gttgttttta agcgccagat
    35521 tcatacattc gtcgagtgaa taaacgggag tttgtgccga taatgttcct gaaagtgcca
    35581 acagcagagg aataatgaag tttttcgatt ttaattttct cacaatttta tatttaattc
    35641 cttcgtcgtc atgaaatact ttgaaaagaa gcggcaatac aatcatggta agcatcgtgg
    35701 aagttatcag cccaccgatg actacggtag ccaacgggcg ttgtacttcg gcacctgctc
    35761 cggatgagat agccattggc agaaaaccca tggcagcagc tgcagccgtg agtataacag
    35821 gtcttaaacg gtccgaagtg ccttttttta ccagttcgtc catatttttc attccggttt
    35881 ctttcaggtg tttcaggtgt tctatcagta ctattccatt gagcacagca ataccaaaca
    35941 aggcaatgaa tcctactcct gccgaaatac tgaaaggcat tccgcgtatc cacaatgcta
    36001 ctattccacc cacaaccgat aaaggtacag ctgtaaaaat aagtatggta tctttcaggg
    36061 atttaaatgc aaaatggaga aaaataaaaa tcattgccag cgctatgggc actgcaaaca
    36121 ccaaacgttt ggtcgcattt tgcaggtttt cgaactgtcc gccatagctg atgtaatacc
    36181 ccggattgag tttcacgttt ttatccacca actgctgaat atcttccact accgattgca
    36241 agtcgcgatt acgcacattg acgctcacta ccacgcgccg gtgggtgttt tcgcgactta
    36301 ttttggcagg accttctgag aaattgattt ctgccagttc gcttaaaggc acctgaattc
    36361 cgttgggtaa tgccacagga agctggcgga tattgtcaat atcggtcctg aaagcctgct
    36421 gcagacgtac aaccaaatca aaacgacgtt cgccttcaaa tacatttccg gcaatttcac
    36481 caccgaaagc ggcagccaga taactgttga gtgttctgat atcggttccg tagtatgcaa
    36541 ttttcgaccg gttgtaagtt acattcattt gcggaagtcc ggtggtcttt tccagaatca
    36601 cgtcgcctgc cccatccact ttttctatca ggtgcttaat ttcttcggct ttttggctca
    36661 gataatccag atcatcgcca aataatttta cggcgatatc agccctaact cctgttatca
    36721 actcattgaa acgcatttca atgggttgtg taaattcata gtcgataccg ggaattacac
    36781 tcagcgcttc cttgaattta tctgcgagtt cttccttcgt tttggccgaa gtccaggtat
    36841 ctttcggttt cagtcggatt atcatatcaa tttcctccat cgacatcgga tctgtgggaa
    36901 cttcggcagc gccgatgcgg caaactattt tttccacttc cggaaattta tccatcaaaa
    36961 tgttttccat ttgtgtggta agttcgatag ttttggtaag cgatgtgccg gttttcagta
    37021 caggctgtat tacaaaatca ccttcgtcga gcgtcgggat aaattctcct cccatccggc
    37081 tgaaaagaaa gacagaaaat atcaacgtaa gaaatgaaag ggtaaaaatc aggacttttt
    37141 tgttcaggga ccatttcaaa acgggttcgt aggtagctct ggcaaaacca agcagtctgt
    37201 cggacaacgt ttttttattc aaatcaagcg gacgcagaaa cagggatgag attacaggca
    37261 gccaggtgag gcaaaaaatc atggctccta tgatggcaaa tccaaatgcc atggccatcg
    37321 ggcgaaacat tttgccttca actccctgca gcgatagtat cggaataaat acgattagga
    37381 tgattacctg cccgaaaatg gccgactgca tcattttcga tgcgcttgtc tgtgttattc
    37441 tgttgttgag attttgtcgt tcttcggccg aaatatcttt aaatttcaat ttatcggccg
    37501 taagctgcac cacgatatat tcaacgatta tcacagcacc gtcaattatt attccaaagt
    37561 caagtgcccc caggctcatc aggttggcat caattccgaa aacgaacatc attgaaagag
    37621 tgaacagcaa tgccagcggt atcatggaag cgataaccag tgctgagcgc aaattgccaa
    37681 gcagaagcaa taccaccaga aaaacgataa tacaaccgaa caagaggttt tcaagtacag
    37741 tcagcgttgt tttgccaatc agttcactgc gttcgagtat gggattgatg aatacacctt
    37801 ccggcagtga tttctgaacc tcatccaccc tttttttcac ttcgctgatt acccgcttgg
    37861 agttggcatc tttcagcatc atgatttgtc cgagcacttt ttcgccttca ccatttgctg
    37921 tgatggctcc gaaacgattc gcgtatccga aatgcacctg tgccacatca tttatcatca
    37981 cgggaattcc tccctgattt ttaactacaa tatgctgaat gtcatccagc gttctgacct
    38041 gaccatcgcc gcgaatgaag taactctcac ttttcttttc gatgtatgca cctcctgaaa
    38101 tgctgttgtt gttttcaaga gcaccgaaaa catccatcag tgtgacaccc agtgcctgca
    38161 ttttcgatgg atttaccgct acttcgtact gtttgaggta tccgccccag ctgtttactt
    38221 ccaccacacc gtcaataccc gaaagctgac gtcgcacaat ccaatcctgt atggtgcgca
    38281 aatccatggg agagtaccgg tcttcgtagc ccggttttac gtccagcacg tattggtaaa
    38341 tttcacccag tccggtggta attggtccca tttcgggagt accgaatcct tcgggtattt
    38401 tggccgaagc actctttatt ttctcggcaa tcagtgttcg gggcaggtaa gttcccatgc
    38461 tctccttgaa aacgatggta accactgaca gcccaaactt ggataccgat cggatttctt
    38521 tcactcccgg cagattggcc atctccaact ccacgggagc agtgataaac tgttcgattt
    38581 cctgcgtgga gaggttgccg gaagtggtaa taacctgcac ctggttgttg gtaatatcgg
    38641 gcactgcacc caccggtatg ttgaaaatgg catagaaacc aaatacaaca atggcaaacg
    38701 tgaatagaac aactatcagt ttatatttca gactgtatcc aataattttt tcgagcatac
    38761 ctgttgttat taatttatca tacaaaatgg aacaaccaac ttcgaaaata atttacctgt
    38821 aaacaaaatt ataaatttat tttattcaat gtataa
//
LOCUS       DAORKP010000003        33865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_18_length_33865_cov_8.803461, whole genome shotgun sequence.
ACCESSION   DAORKP010000003 DAORKP010000000
VERSION     DAORKP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 33865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33865
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_33865_cov_8.803461"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(766..1722)
                     /gene="hemH"
                     /locus_tag="PLN75_00400"
     CDS             complement(766..1722)
                     /gene="hemH"
                     /locus_tag="PLN75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRG02605.1"
                     /translation="MIENPGNNTPKTAVLLVNMGAPVSEQGMKVFLRRLFRDKAIVQA
                     PEPVRRLLAATVSRMRYKSSWRKYLTIGGSPLLNSMNKTAAELQKLLSSNYQVRCAFS
                     YSEPFIENEITELTLDGFEDFVIISMYPQSGFTTTGSIAAVLKRIQETNNGLRIRFVE
                     DYHDNPFFVQLWTNLIIRKIAETGYRHPILLFSAHAIPQSGIKRGDSYPLRIQKSAQL
                     IADALNLPYRVGYQSKLGPVKWTEPSTSEVLKKLSANNASEIIIVPLSFVNENLETCY
                     DLDIKLIPYAINILKIGKICRIHIPESDNTLVRMYRSFIEPY"
     gene            complement(1793..2098)
                     /locus_tag="PLN75_00405"
     CDS             complement(1793..2098)
                     /locus_tag="PLN75_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRG02606.1"
                     /translation="MIKHIVCFRLADVAEGKSKAENAQIIKEGLENLINLIPQLRKIE
                     VGINHPATPESNFDIMLYSEFDTIEDLNMYQEHPEHKKVGAYIVKVKTDRVCVDYEV"
     gene            complement(2095..3720)
                     /gene="nadB"
                     /locus_tag="PLN75_00410"
     CDS             complement(2095..3720)
                     /gene="nadB"
                     /locus_tag="PLN75_00410"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRG02607.1"
                     /translation="MAKKYDFLVVGGGIAGISFALKASKFGKVALLCKTTLEDSNTSY
                     AQGGVATVMYEPDNYEKHIEDTLTAGDGHCDRDAVELVVKNARKEIDQLLEWGMDFDK
                     TEDGKLFDLHKEGGHSEFRILHHKDNTGFVIQESLNKQVRNHPEIDVFEHYFAIEILT
                     QHHLGEIVTHHTPNIECYGVYALNQKSNHIHTFLSKVTVLATGGIGSVYQASTNPAIA
                     TGDGVAMVHRARGVIRDMEFVQFHPTALYHPGDKPVFLITEAIRGYGAVLRGQDGKEF
                     MQKYDSRGSLAPRDIVARAIDNEMKIHGDSFEYLDVTHKNAEETKEHFPMIYQKCLEY
                     GIDITKDYIPICPTQHYMCGGIAVDLNGHSSINRLYAIGECSATGLHGANRLASNSLI
                     EAIVYADVAVKHAVPRISQITHNELIPDWNDEGTSLTEEMVLITQSFREVEQIMSTYV
                     GIVRSNLRLQRAMSRLEILFRETESLFSRSVVSREICELRNVISVSYLIIKQALARKE
                     SRGLHFTVDYPKKMEEEKNYFETIQKRKKHPKK"
     assembly_gap    3750..3849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3861..5288)
                     /gene="rlmD"
                     /locus_tag="PLN75_00415"
     CDS             complement(3861..5288)
                     /gene="rlmD"
                     /locus_tag="PLN75_00415"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRG02608.1"
                     /translation="MAKNKKQLPVLEQVMVTDLAAEGKAIAKVNDVVVFIPFAVPGDV
                     VNLQVTRKKSSFMEARVIDMVQPSEIRQESFCEHFGICGGCKWQILPYEEQLKYKQKE
                     VENNLRRIGKVDLPEISPILGSAKTTFYRNKLEFTFSNRRWRTFEEINSAMEFDTMNA
                     VGFHIPGMFDKVLDINKCWLQDDIQNRIRNEIRSYALQNNLTFFDLRLQEGFLRTMMV
                     RTTSTGELMVILVFFHEDKSEREKLLQHLADKFPEITSLLYIINSKANDTITDQEVLT
                     FKGNDCIYEEMEGLRFKIGPKSFYQTNSGQAYELYKVARSFAGLTGKELVYDLYTGTG
                     TIANFIAGDCSKVIGVEYVPEAIEDAKVNSSLNGIKNTLFYAGDMKNVLNDEFIRIHG
                     KPDVIITDPPRAGMHEDVVKVMLLAEPERIVYVSCNSATQARDLALLDEKYRVAAVQP
                     VDMFPHTHHVENVVLLRIRNANCAN"
     gene            5468..5995
                     /locus_tag="PLN75_00420"
     CDS             5468..5995
                     /locus_tag="PLN75_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRG02609.1"
                     /translation="MKVRLFLILSLAGLFISFSSCRKVTSAAAELLSTTDTAGFIFPA
                     PLGSVSDFEEILTAEQIRELDSIIVQFENNSDNKINIVTTKSIKPYTSLDEYSADLLK
                     NWENDDVEVHTVMLVYSKKLNEVEITAGDELKRKLTDKECKRIAKKTIVAETEKSDYF
                     TGLKTALIKIITEIR"
     gene            complement(6291..6497)
                     /locus_tag="PLN75_00425"
     CDS             complement(6291..6497)
                     /locus_tag="PLN75_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02610.1"
                     /translation="MCTGIEIPVAAFFVSATRRPCLRQVELRFGETYHLKVGYLRKLP
                     HARDRKTIHFVILHIGRLRNQSGR"
     gene            complement(7640..10108)
                     /locus_tag="PLN75_00430"
     CDS             complement(7640..10108)
                     /locus_tag="PLN75_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG02611.1"
                     /translation="MPETYSAHEYASLFATLTNQNVFLTGKAGTGKTTFLHRLRETSP
                     KQIAVVAPTGVAAINAGGTTIHSFFQLPLTPFIPTEMGRKELIGKMKMQSNRRKVIRE
                     LELLVIDEISMVRADILDAIDTVLRHVRYRHNEPFGGVQMIFIGDMYQLSPVAQNEEW
                     QILSRFYQGVYFFNSLVFQQKPFVHIEFEKIFRQKDIEFIQLLNEVRNNRLSAESLAL
                     LGKCYNPFFVPATDDTYIILTTHNYKADRINREELDKIKSGTKIFEAVVTGDFPERSF
                     PADDKLELKIGAKVMFLRNDSENPRRFFNGKIGLITGWDKDIIRVKCPEDNEPIAVLP
                     VEWENIRYQTDESTLLVKEDVLGTFRQYPLRLAWAITIHKSQGLTFDKAVIDAESAFA
                     AGQVYVALSRCRSLEGLVLQSKINAGSIQNDNEILRFSSRHLNVDELENRFSSSRQEY
                     FLSLLLQVFDFRQMVAVSGRWLTSTADAETSFGSETLEFLRNINAQINAIHDVGARFG
                     TQISQILKHNEFNHDALNLRLEAADTYFSEKLNLLTDTLRQSPATTDSRENAKEFDQY
                     YLDIFTFAAQKKHWISGIRDGFGIEKYFQLRKSFELPAIKYTSYSRHQKQEKLSSRRP
                     DLMGILYELRNSIVDDTGLPVYMVAGSHSIAEMADYLPQTKSDLLKISGFGEARVEKF
                     GELFLEEIRDYCLENGLTSAMDELAGREKRKRKDKKEKSEKKIKGSSALLSFQEYKSG
                     KSVEKIAAERSLSVGTIYGHLGRYVTKGELTAEEFVPPGELQRAKSILEKTDEDVSIY
                     SKLKEHYSQGEITIISAWLRKEQS"
     gene            complement(10305..11972)
                     /locus_tag="PLN75_00435"
     CDS             complement(10305..11972)
                     /locus_tag="PLN75_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRG02612.1"
                     /translation="MEKSLNFIEQIVEDDLAAGVNDGRIQTRFPPEPNGYLHIGHAKA
                     VCIDFGIAERYNGTCNLRFDDTNPVKEDVEYVDSIMEDIKWLGYKWNNVYYASDYFQQ
                     LWDLAVRFINEGKAYVDEQSSEIIAQQKGTPTVAGTESPYRNRSVQENLDLFMKMSAG
                     EIPDGKMVLRAKIDMASPNMHFRDPILYRVITTHPHHRTGYTWKVYPMYDFAHGQSDY
                     FEGVTHSICTLEFVVHRPLYDWFIDQFKETEYRPKQIEFNRLNITYTVMSKRKMLQLV
                     QEGLVSGWDDPRMPTLCGLRRRGYSPESIHNFIDKIGYTKVEGIIDLGLLEHAVREEL
                     NKTSTRVSAVINPVKLIITNYPEGKTEMMEAVNNPEDENSGTHTIAFTRELWMEREDF
                     MEEAPKKFFRMTPGQEVRLKSAYIVKCTGCKKDEDGNITEVYAEYDPLTKSGMPDANR
                     KVKGTLHWLSAAHSLPAEVRLYDRLFSVENPSADERDFRELINAGSLEVKTNCYVEEY
                     LKDAQPLDRFQFQRIGYFNVDPDSTAEKLVFNRTVSLKDSWAKEQNK"
     gene            complement(12106..12885)
                     /gene="lpxA"
                     /locus_tag="PLN75_00440"
     CDS             complement(12106..12885)
                     /gene="lpxA"
                     /locus_tag="PLN75_00440"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRG02613.1"
                     /translation="MKQPLAYIHPDAKIARTVVIEPFVTIDKNVEIGEGTTIGSNVTI
                     LEGVRIGKNCRIFPGAVIGGIPQDLKYKGEESLVIIGDNTTIREFVTVNRGTVSKGKT
                     VVGDNCLLMAYCHVAHDCVLGNNVIMSNACQLAGEVEIDDYAVLGGGTMIHQFSRIGA
                     HTMIQGGSRVTKDVPPFITAGRDPLSYAGVNSIGLRRRGFTNEQIRDIQDVYRFIYQS
                     GMNTTHSVERVQAELPATNERDEILQFIKNSPRGIIKGYFD"
     gene            complement(13039..14424)
                     /locus_tag="PLN75_00445"
     CDS             complement(13039..14424)
                     /locus_tag="PLN75_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRG02614.1"
                     /translation="MATQRTIKSAFGLKGKGLHTGVEINITFLPAPVNHGIRIRRTDL
                     PGQPDITALADYVVSTNRGTILKRGEMQVSTIEHAMAALFAWQVDNCLIEVDAPEFPI
                     LDGSARYYYEEIEKAGVEEQLKAEKDIYIVKKRIDYHDVETNAKITILPDDHFSVDVY
                     VDYNSPVLPAQYATLEDLSDFGKEIAGCRTFVFVREIEPLLSQNLIKGGDLENAIVIY
                     DKEMPQDELNKLADLMKHPRVKVDELGIISGDLKFKNEPARHKLLDIIGDLALAGKQI
                     HGKVIARFPGHKVNTEVAKLIRKEIKGQEVQSPLYDDTVPPLLDSNAIRKMLPHRWPF
                     LMVDKILEIRERSIIGVKNISSNETVFMGHFPDEPVMPGVLLIEAMAQTGGLLVLSQL
                     EDPELYSTYFMKIDNVKFRQKVVPGDTVVFHVSLMDEIRRGIATMKGYAFVGGVIATE
                     AEFMAQVIKNK"
     gene            complement(14643..15698)
                     /gene="lpxD"
                     /locus_tag="PLN75_00450"
     CDS             complement(14643..15698)
                     /gene="lpxD"
                     /locus_tag="PLN75_00450"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRG02615.1"
                     /translation="MEYTAQQIAEYLHGEIIGDPAVKVREFSKIEEGENGTLSFLSNP
                     KYSHYLYESNASIILVNRDFEPEKEVKATLIKVDNAYESLAMLLSSIEQLKPKKNGVS
                     ALAHISPSAKIGINVYIAPFVFVGEGAEIGNYASIDASGYIGENVKIGENTQLHAGVR
                     VEKECIIGKNCILQAGAVVGSDGFGFAPANDGSYRKIPQVGNVVIEDDVEVGANTTID
                     RATLGSTIIRRGVKLDNLVQIAHNVEIGENTVIAAQSGIAGSTKIGKKCVLAGQVGIS
                     GHIEIADGCVFGAQTGVPNNVKEPNSIMQGYPAVPVGTFRRASVVYKNLPDLQRTVNE
                     LQKQIENLKLVLGNTKH"
     gene            complement(15772..16995)
                     /locus_tag="PLN75_00455"
     CDS             complement(15772..16995)
                     /locus_tag="PLN75_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRG02616.1"
                     /translation="MKRKIINDPVFGFINIPNDFLYDIIQHRYFQRLNRIKQLGLSSF
                     VYPGAQHTRMLHSLGAMHLMGEAIAQLRLTGQNISATEEDAARACMLLHDIGHGPFSH
                     TLENSIVQGTSHEDISLWMMQRINDDFDGRLETALQIFTNQYPKHFLHQLVASQLDMD
                     RLDYLSRDSFFCGVSEGIIGASRIIKMLNLHNDKLVVEAKGIYSIEKFLVARRLMYWQ
                     VYLHKTSVAAEKMLINILKRAKEIASQGVELFGSPALKYFLYNNISRNDFIHTAETLD
                     HFAMLDDADVLCAIKVWSEQCKDTVLAALCSQFANRKLFKVESLTGKQQGDELREKYL
                     KKYQEHFGISAYEAAYFIGEEVVSTDTYSPEDESISILYKDGTIKDIADASDMLNIQV
                     LTKTVEKHFLCYCKI"
     gene            complement(17333..18778)
                     /locus_tag="PLN75_00460"
     CDS             complement(17333..18778)
                     /locus_tag="PLN75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRG02617.1"
                     /translation="MYMKDLQPQIVWNHFYEITQIPRPSKKEEKIRAYLQEFGKKNNL
                     PTRTDEAGNVLITKPATPGYENKKTVILQSHMDMVCEKNSNVEHDFEKDPIQTEIDGE
                     WLRAKGTTLGADNGIGMALMLGVLASDNIKHPPLECLFTTDEETGLTGAFALSNDMLT
                     GDVLINLDSEDDGEIFVGCAGGIDTTATFNYETKKTPKGYFAFSVSVSGLSGGHSGDD
                     IDKHRANANKLLNRILWNMLRDMDLRLVKIDGGNLRNAIPREASATACVPYKEKENVR
                     VIFNRVVSDIEEEFQNIETKMRIELHSDETPKETLTKKSTRKLLNALYACPHGIKKMS
                     MDMPGLVETSTNLASVKMKEGEQIIITTSQRSSVESAKYDIAHQVEAVLKLAGCKVTH
                     GDGYPGWKPNLNSEILKVAEKSYKKLYKTEPKVKAIHAGLECGLFLKKYPHLDMISIG
                     PTMRGVHSPDERLHIPAVGRAWEWLSDILAY"
     gene            19129..19887
                     /locus_tag="PLN75_00465"
     CDS             19129..19887
                     /locus_tag="PLN75_00465"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRG02618.1"
                     /translation="MKNFLQIIKDFDTITLPEMGKVMLLNRTDVKFSFHRDLLSDILT
                     LAKADYLSLVINEQHYADYETHYFDTPEYGFYLDHHNKRLNRYKVRTRSYVNSDLHFF
                     EIKFKSNKGRTIKSRMKIAQPDSCITEECAEFLKTKAGISAENLKRSIQVNYKRITLI
                     NKGMTERITIDFDMSYLIDGARYEYPELIILEVKQNKSSQSRFMEIMHEQHLHRVSLS
                     KYCFGIASYVDGIKKNYFKNQIRYVKKICNTAFN"
     gene            19853..20629
                     /locus_tag="PLN75_00470"
     CDS             19853..20629
                     /locus_tag="PLN75_00470"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRG02619.1"
                     /translation="MLKKFATQHSIKFIVLLSFFVIIPFSPLPAQDELMMEETGQMFN
                     EEPVASLSPDSVLRAEFKAVIKPPKKEEVELGLTFFLSLLLDLLMVFLIIRTIYYPNY
                     RKLDTVFTFVMFNVSIFLLISLLNQIKISMGAAFGLFAVFTMLRYRTAGISIKDMTYL
                     FIFIALGLISGVRLQIHDLLIIYAVIYVMLFLLDTRYFIKRESTKTIFFDNIDLINAS
                     KEKELIEELKLRTGLKIHRVSIEDISYLRDSATITIYYYD"
     gene            20629..21333
                     /locus_tag="PLN75_00475"
     CDS             20629..21333
                     /locus_tag="PLN75_00475"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRG02620.1"
                     /translation="MRKITFVLFLLAFLTGVKTMAQAQYGTWTSVGVEKKLGKWKLEA
                     DTEIRTIYYLRLINRWSIGLDAEYKIAKPLEVGLSYTLMNVLDQKYLNYQFRNRFNAG
                     ITGKQKWGRFDFSLSEGLQLTTKNASKRIDDYGNTDTYKINPALMWKNSMQAEYDIPK
                     SKLTPGGEIESYYELNNPDGNKFDKLRYSLFLKYKINKHNSLKLAGIYNDELGSDDAD
                     YSGKYIVDLKFSHTLK"
     gene            21340..22848
                     /locus_tag="PLN75_00480"
     CDS             21340..22848
                     /locus_tag="PLN75_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRG02621.1"
                     /translation="MNKLLKQLFMLTAIAVIVAGCDPENVIGKDTTKSETATDYEWDN
                     SNVKTIVFNQNAITSTSKDVLIQGTTATITSAGYYNISGNLSDGQLVVNAPKAKLKIM
                     LSGVTMANSTTSPFFIQAASKVIVFLKSGTVNTISDPAIYTNTGEPQAALFSNAYLAF
                     TGEGTLNVNGNHNDAISSDDEVIINSGNFNIQAPDDGIRGKNYVLIHDCNLVAAVNTG
                     HALKSDNTVTDGRGYVKIDGGNISLTSAQGDGINTTKRILIENGKLTLTAYESQGLRS
                     DSLVHISGGEITVMKNSRQGIKSPYIKLTGGTTQITSNKIAVNATFETAATQPDSSLL
                     TVSGGNLYINTLSHGLVSDGNIHIDGGTTVVQGPYDNSKVAVSTLGELKITNGMLVAS
                     GPNAGSLIKSPDNTSTQNSLKITSGTLGTNLINIQDAGGNSLVTFKPIKSAYYLLFSS
                     PALKTGTTFSVFTGGSYSGGTTINGFSTGGIYTPGTKKSTFTLSEKVTPVNF"
     gene            23014..24420
                     /locus_tag="PLN75_00485"
     CDS             23014..24420
                     /locus_tag="PLN75_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG02622.1"
                     /translation="MNWTKKQTSTLAVVAITSFMGTFLISSINIALPSIEKTFGLNAI
                     MLSWVVTSFLLATAMFLLPAGRWSDISGVVRLYKAGLIIFTLSSLLCGIAPSGGWLIA
                     ARYLQGIGAAFTNTTGQAILVGSFPPQNRGSVLGISVSSVYLGLSFGPFVGGLLTQQI
                     GWRSIFMAAALIGLVSTVVSFLYLGKDEQTGQTKQKMGLKGVIFFMLGLVCMVYGSSL
                     IPATSGWLIMAAGLAALILFWKIESKSKMPVFDTGLFTKNRMFAFSNLAALINYGSTS
                     AIVFFLSLYLQKIQHLTPQQAGLILIAQPIMMTLFSPLVGRLSDRHQPRYFATSGMAM
                     CSVGLASMSFLNEVTPHWVIISILIWIGIGFALFSSPNMNLIMSSVNRTQYGLASGTA
                     ASMRVIGQITSMTIVTLLFAGLFSGKSIEKVSDSLFLTAMKWGFITFSLTSLVGIYFS
                     YVRGNVKRKTPLPADSQE"
     gene            24506..25162
                     /locus_tag="PLN75_00490"
     CDS             24506..25162
                     /locus_tag="PLN75_00490"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRG02623.1"
                     /translation="MITIFIIALTALISYLAFNNSSYMNKLIFYPPAVKRGGWYRLVT
                     YGVLHADFTHLIFNMLTLYIFGSYIEKFFRAILGDQAGGFWYIIMYISALIISILPTY
                     LKEKNNDNYRGLGASGAVSAVVFAYILINPMSFMGVMFIPVMLPAFLFGILFVVLSVV
                     LQKKHSGGINHSAHIAGGIFGMVFIVILFALLSDINIIRHFADQIQISSISDLIHIGY
                     "
     gene            complement(25226..25789)
                     /locus_tag="PLN75_00495"
     CDS             complement(25226..25789)
                     /locus_tag="PLN75_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRG02624.1"
                     /translation="MKRFFTVLLAALFVVGLSAQTIYKVDPYHSSINFKVKHLGVSFV
                     TGKFEKFDGMIEGSDVKNARIDFTVDVNSINTGVEMRDKHLRSADFFDVPKFPSMKFT
                     STSIKKSGKIYKLNGNLTIKDVVRPVVFDVVMNGPVKGKNGGDIYGFSAKTTINRFDY
                     GVAYDPELKSVGKDVHITLYLEFGQQK"
     gene            26039..27061
                     /locus_tag="PLN75_00500"
     CDS             26039..27061
                     /locus_tag="PLN75_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02625.1"
                     /translation="MKKQVLKSIAAATILLAAFTFTGCGTYYGATDQGGYYDDYSYNS
                     PTWGPSYYAGARYYYFPDIEAYYDLASRDFIFLNNGQWIFVQTLSPFYSSFDLYSSFI
                     IIINRGVYQPWMHHHYYNSHYPRYYYIDYYDYSNIPYVRGFNENQKGAIYWPENQRER
                     ARAWDDRNIRNNRRFSYSEADRRVQQETTRQMSTQRTSRTDIGRSNTAERTRTTDADR
                     SRTETRTSDQTRTQTRTTTDQTRSATRTDIPETTRSNTSTTRTERTTASPRTDTGTNT
                     RSDAQTSTRRTTDTNYYGEPIGRPVRVERQMRSTETTTTRSRSTDTNTDTNTERSSSS
                     RTSGRR"
     gene            27271..29421
                     /gene="pnp"
                     /locus_tag="PLN75_00505"
     CDS             27271..29421
                     /gene="pnp"
                     /locus_tag="PLN75_00505"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRG02626.1"
                     /translation="MNVVTKTITMGDGREITIETGKLAKQADGSVVVRMGNTMLLATV
                     CAAKDATPGTDFMPLSVEYKEKYGAAGRFPGGFTRREGRASDYEILIARLIDRALRPL
                     FPDDFHAEVFVNVNLISADGVDMPDGLAGLAASAALAVSDIPFHGPISEVRIGRVNGE
                     YLVNPTFAQKEEADLDIIVAATADNIMMVEGEMKEVSEAELLEAIRIAHEAIKIQCQA
                     QVELMEEVGKTEKRTYSHEENDEELKQDIHTKTYDKVYAVAASCNADKHSRIENFEAV
                     KAEYIASLEPEVAEEKKDLINRYYHAVEKEAMRRSILDEGKRLDGRKTTEIRHIWSEI
                     DYLPGAHGSAVFTRGETQSLTTVTLGTKMDEKIIDEALIKSKDRFLLHYNFPPFSTGE
                     AKPQRGVGRREIGHGNLAYRALKPMIPTDYPYVVRVVSDILESNGSSSMATVCAGTLA
                     LMDAGVQIKKPVSGIAMGLISENKGQNYAILSDILGDEDHLGDMDFKVTGTRDGITAT
                     QMDIKVDGLSFEILENALNQAKAGRMHILDKIQETISEPRADLKPHAPRIVVMFIPKD
                     LIGAVIGPGGKIIQNIQAETGAVVSIEEIGEQGRVEIASANKESLDAAVAKIRGIVAI
                     PEVGETYNAKVKSIMPYGAFVEFMPGKEGLLHISELKWERVETMDQAGLKEGEMIEVK
                     LLDIDEKTGKFKLSHKALLPRPPREERPPREPRG"
     gene            complement(29581..30465)
                     /locus_tag="PLN75_00510"
     CDS             complement(29581..30465)
                     /locus_tag="PLN75_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRG02627.1"
                     /translation="MSHNLLKGKRGIVFGALNNMSIAWKVAERAVEEGAIITLSNTPL
                     AVRMGTTQELADKLNAKLIPADATSVEDLENVFKESMEALGGKIDFVLHSIGMSPNVR
                     KKRTYDDLDYEMLSTTLDISAISFHKMLQVAKKMEAIADGGSIVALSYMAAQRTIYGY
                     NDMADAKAMLESIARSFGYIYGREHNVRVNTISQSPTMTTAGSGVKGFDSLVDFSERV
                     APLGNATADDCADYCVVMFSDLTRKVTMQNLFHDGGFSSMGMSYRAMEQYLKSFDCPE
                     SDLKFKEFKDEKGKIIYG"
     gene            30745..31257
                     /locus_tag="PLN75_00515"
     CDS             30745..31257
                     /locus_tag="PLN75_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02628.1"
                     /translation="MNYDDNDPFSSGNHYRQMPVFKKGQEIVDLIMQIAALIPEDDEM
                     LQEVMGMILGDAYMLTAKIANAEGSDLYDHKMQNAAIIRKAAMDLFVSYHSLKMHGFE
                     HAEYYMMVRDLIEEYRLLFVEWVDGFDKSCYLVDRWNVFNPPGVHYTDTDPDIDLGFD
                     PEDFEEDDDF"
     gene            complement(31346..33505)
                     /locus_tag="PLN75_00520"
     CDS             complement(31346..33505)
                     /locus_tag="PLN75_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRG02629.1"
                     /translation="MKVYQTNEIKNISLLGSSGAGKTTLAEAMLFESGVIKRRGSVKN
                     QSTVSDYFPVEKEYGYSVFSTVLSYEWKNKMMNVIDCPGSDDFIGGVISALNVTDMAL
                     MLIDATSGVEVGTMNQFRYTEELHKPVVFVINQLDHEKADYENTLTGLKEMYGSKVIP
                     VQYAVSSGASFDAVIDVLKQKMYKWKPGATAPDVLEIPDSEKAKAADVFQQLLEAAAE
                     NDDSLMEKFFDQGTLSEEEMRDGIHKGMISRSLFPVFCASGEKNMCVHRIMNFLSVAA
                     PSPDEMPAPQNADGEEVKPDSGKPTSLFFFKTTVEPHIGEVSYFKVMSGKLKEGDDLV
                     NANRGTKERLSQIQTVAGQIRNKAEVLEAGSIGAALKLKDVRTGNTLNSKDAENKFDF
                     IKYPDPRYRRAIRPLSESNSEKMSEALSRMKEEDPTWVVEVSKELKQTIVSGQGEFHL
                     KTLKWRIENNDKIEIEFIEPKIPYRETITKASRADYRHKKQSGGAGQFGEVHLIVEPY
                     FEGMPAPDVFRFGNQEYKISNRDTQEIPLDWGGKLVFINSVVGGAIDTRFLPAILKGI
                     MARMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFMLAGRNAFSTAFKEAGPKILEP
                     IYDVVVSVPSEYMGDVMGDLQGRRGMIMGMESEKGFEKLKARVPLKEMGSYSTSLSSI
                     TGGRGSFNMKFASYELVPSDVQDKLLKEYEAQKKEEE"
BASE COUNT         9528 a         7411 c         6885 g         9941 t
ORIGIN      
        1 tattgaattt ataccatcga aatattttga tttgaaaaat tgaattcata gcaagtgcgt
       61 taattattta tcttgtgctt aaaaaaacaa ttgcttccgg tcacttattc cactcttttt
      121 cacacacttt ttaatttata aaactgaaat taattttgct gtttcagtaa caaataaaca
      181 acaattctta taaatagttt accaactatc ataaaataat tgaaaaaccg gatgaatttc
      241 ttttccaggt catccctttt ctgaggagtt ggacaagcat tggttaagaa ttatttttca
      301 ccatcgaagt attcgtaggg catttttgct gcaacctatt cccggaaaga atcatcgggc
      361 attacaggct gataatgctt atgtgcagag aaaaaacagt agaataaaaa accaatcaga
      421 tgtggcatcc agccatttag aatgatgata atttcatttc tttttttcac tacataacgg
      481 caagaccata ttaatctgat aaaaaagaga tgccaaccaa atttgagtaa ttttcctggc
      541 tattttgcat ttgtttttct ataaatggtt aaatgactca taaatgtgaa ctttaggcgg
      601 agaacaatta ttgtcatttt tttccatcgt agcaaaaaaa aaggggggct ttcctctctg
      661 actctgaaaa aaataatata tactatcctg ccgtcaaagt tgcatttact aattgaattt
      721 acataaagcc gtatgtgtac agaactgaat taacctaatg cggcatcagt atggttcaat
      781 aaaactccgg tacatacgta ccagcgtatt gtcagattca ggaatgtgaa tacggcagat
      841 tttcccgatt ttcaggatgt ttattgcata tggaatgagt tttatatcga ggtcgtaaca
      901 tgtttccagg ttctcattca caaaactcaa aggaacaatg ataatttcac tcgcgttgtt
      961 agcacttaat ttcttcagaa cttccgaagt ggagggttct gtccacttca caggtcctaa
     1021 tttcgactga taaccgaccc tgtaaggcaa atttaacgcg tctgcgatga gttgtgctga
     1081 cttctgaatt ctgagtggat agctgtcgcc ccgtttaatg cccgattgtg gaattgcgtg
     1141 tgcactgaaa agtaaaattg gatgcctgta accggtctct gctattttcc ggatgatcag
     1201 attcgtccag agctgcacaa agaagggatt atcgtggtaa tcctccacaa agcggattcg
     1261 tagtccgttg ttagtttcct gtattctttt cagtacagca gcaatgcttc ccgttgtggt
     1321 aaagcctgac tgtggataca tggaaataat cacgaagtct tcgaatccgt ccagtgtcag
     1381 ttcggtgatt tcgttttcga taaacggctc ggaatacgaa aaagcacacc gaacctgata
     1441 attgctgctg agaagcttct gcaactctgc agccgtttta ttcattgagt tgagcaaagg
     1501 cgatccaccg attgtgaggt atttcctcca cgatgattta taacgcatcc ggctcacagt
     1561 cgctgcaagc agccgacgca ccggttccgg cgcctgaacg atagctttat cacgaaacaa
     1621 ccgccttagg aaaaccttca ttccctgctc ggagacaggc gcacccatat tgaccagcag
     1681 aactgctgtt ttcggggtat tattaccggg attctcaatc attgaatgct ttgtaaattc
     1741 tgtaatcaga aacaatttcc tcaagtcaaa tttcggcgat tctccgtcat ttttacacct
     1801 cgtaatctac acaaacgcga tctgttttga ctttgacaat ataggcgccc acttttttgt
     1861 gttccggatg ctcctggtac atgttgaggt cttcgattgt atcaaattcg gagtacaaca
     1921 tgatatcaaa gttggattcg ggtgtagccg gatgatttat ccccacttca attttacgaa
     1981 gttgcggaat gaggttaatc aggttttcaa gtccttcttt gattatctga gcattttctg
     2041 ctttcgattt tccttcggca acatcagcca gccggaaaca aacgatgtgt ttaatcattt
     2101 ttttggatgt ttttttcttt tctgaattgt ttcaaaatag tttttttctt cttccatttt
     2161 cttcggatag tcaacggtga aatgcaatcc gcggctttct ttccttgcca gtgcctgttt
     2221 gatgatgagg tatgaaacgc tgatcacgtt gcgcagttcg catatttcgc gcgatacgac
     2281 cgagcggcta aaaaggcttt cagtttcacg gaaaagtatt tcgagacggc tcatggcacg
     2341 ctgcaaacgc aaatttgaac gtacaatgcc cacataagtg ctcattatct gctccacttc
     2401 acggaaactc tgggtgatga gtaccatttc ctcggtgagc gaagttccct catcgttcca
     2461 gtcgggaatc agttcgttgt gagtgatctg actaatgcgg ggtacagcat gttttacggc
     2521 aacatctgca tagacgatgg cttcgatgag tgaattggat gccaggcggt tggctccgtg
     2581 gagtccggtg gccgaacact caccgatagc gtacagccgg ttgattgagg agtgtccgtt
     2641 gagatcaacg gctatgcctc cgcacatata atgctgtgtg ggacagatgg gaatatagtc
     2701 tttggtgata tcaatcccgt attccagaca cttctgataa atcatgggga aatgttcttt
     2761 tgtttcctct gcattcttgt gggttacatc caggtactcg aagctgtcgc catggatttt
     2821 catttcgttg tcgattgccc ttgccacaat atcgcgggga gccagtgaac cgcggctgtc
     2881 gtatttctgc ataaactcct ttccatcctg tccgcggagc acggcaccat aaccccgaat
     2941 ggcttcggta atgaggaaaa cgggtttatc gcccggatga taaagcgctg tgggatgaaa
     3001 ctgcacaaat tccatgtcac ggattacgcc gcgggcacgg tgtaccatgg ctactccgtc
     3061 gccggtagca atggcaggat tagtggatgc ctgataaacg ctgcctattc caccggttgc
     3121 cagtacggta actttggaga ggaatgtgtg gatgtggttg cttttctggt taagagcata
     3181 taccccgtaa cattcgatgt tgggtgtatg atgggttaca atttcaccca gatggtgctg
     3241 tgtaagaatt tcgatggcaa aatagtgttc aaacacatca atttcgggat gattccttac
     3301 ctgtttgttc agactttcct gtatcacaaa gcctgtattg tctttgtgat gcaagatacg
     3361 aaattcggag tgtccgcctt ccttatgcag atcgaacagt tttccgtctt cggttttgtc
     3421 gaaatccatc ccccactcca gcaactgatc gatttcctta cgtgcatttt tcactacaag
     3481 ttccaccgca tccctgtcgc aatgtccgtc gccggcagtg agggtgtctt ctatgtgctt
     3541 ttcgtaatta tcgggttcgt acatcacggt agccactcca ccctgtgcgt aggatgtatt
     3601 gctgtcttcg agcgtagttt tgcacagcag agccacttta ccgaatttgg acgctttcag
     3661 ggcaaagctg atgcccgcaa ttccacctcc aacaacgagg aaatcatatt tttttgccat
     3721 tgtctcaaat gtgatttatt tgatttttan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnc gtaattcgtc ttaattcgca caattcgcat ttcttattcg taataaaact
     3901 acattttcca catgatgggt gtgcggaaac atatccaccg gttgaactgc tgcgacccga
     3961 tatttttcat ccagcaaagc caaatcccgt gcctgtgtgg ctgagttaca gcttacatac
     4021 acaattcttt ccggttcggc caggagcatg actttcacca catcttcgtg catgcctgcc
     4081 cggggtggat cggtgatgat gacatcgggt tttccgtgta tgcgaataaa ttcgtcattc
     4141 aatacatttt tcatatctcc ggcataaaaa agcgtattct ttatgccatt cagcgatgaa
     4201 ttaacctttg catcttcgat ggcttcgggc acatactcaa cgccaattac tttactacaa
     4261 tccccggcaa tgaaattggc aatggtacct gtgccggtgt acaggtcata caccagttct
     4321 ttaccggtca gtcctgcaaa actgcgtgcc accttgtata gttcataagc ctgcccggaa
     4381 ttggtttggt agaatgattt gggtccgatt ttgaatctca atccttccat ttcttcgtaa
     4441 atacaatcgt tgcctttgaa ggtcaacact tcctgatcag ttattgtatc gttggctttt
     4501 gaattgatta tatagagcaa cgaggttatt tccgggaact tatcagccag gtgctgcaga
     4561 agtttttctc tttctgattt gtcttcgtga aaaaacacaa gtatcaccat cagttcgccg
     4621 gtggaagtgg tgcgcaccat catcgttcgc agaaatcctt cctgaaggcg taaatcgaaa
     4681 aatgtgaggt tgttttgaag cgcgtatgag cgaatctcgt tccgaatccg gttttgaata
     4741 tcgtcctgca gccagcactt attgatatcg agcaccttat caaacatgcc cggaatatga
     4801 aatccgacgg cgttcatcgt gtcaaattcc atagcggaat taatttcttc gaaagttcgc
     4861 cagcggcggt ttgaaaacgt aaattcgagt ttattgcgat aaaaggttgt ttttgcagag
     4921 ccaagaattg gagatatttc gggcagatcc acttttccta ttctccggag gttgttttcc
     4981 acctctttct gcttgtattt aagctgctcc tcataaggca aaatctgcca cttgcaccct
     5041 ccgcaaatgc cgaaatgttc gcaaaaagat tcctgcctta tttccgacgg ctgcaccatg
     5101 tcaatcacac gggcttccat gaaactgctt ttcttacgcg taacctgcag gtttaccaca
     5161 tcacccggca cagcaaaggg tataaacacc accacatcgt tcacttttgc aattgctttt
     5221 ccttcggcag ccaggtcggt taccatcact tgttcaagta caggcagttg ctttttattt
     5281 ttagccattt catttttggt ttaacactac aaaggtaaag attttttgag ataaatattt
     5341 tactgaaaag caaaacttgt atttctgttt ttgttttcaa ttctttcagt aattttaata
     5401 aaaagttaag ttcaaaatgg attacctttg tggataatat ttacctaacc atatttaaac
     5461 acagaatgtg aaagtcagat tgtttcttat attaagtctt gctggtttgt ttatcagttt
     5521 cagctcgtgc cggaaagtga cttctgcagc agctgagctt ctgtccacta ccgatactgc
     5581 cggatttatt tttcctgctc cgcttggttc ggtgagcgat tttgaggaaa ttctgactgc
     5641 agagcaaatt cgtgaacttg acagtataat cgttcagttt gaaaacaatt cggacaataa
     5701 aatcaacatt gtaaccacaa aatcaatcaa accatataca agcctggacg aatacagtgc
     5761 tgatctgctg aaaaactggg aaaatgatga tgtcgaagtc catacggtaa tgcttgttta
     5821 ttcgaagaag ctgaacgaag tggagattac tgccggtgat gaactaaaaa gaaaactcac
     5881 cgacaaagaa tgtaaacgaa tagcaaagaa aacaatcgta gcagaaacgg aaaaaagtga
     5941 ttactttaca ggtctgaaaa ccgctctgat aaaaataatt acagaaataa gataaacact
     6001 tatcaacaat ctcacccatc acatactcaa tatcaaacac ttaacctgtt gataactctg
     6061 ttgataactc acctcataac ttgttgacac aaacaggtaa aaaaatttgc aaaacgatat
     6121 ttcgatgtat tatatttgca acatgagttt cggctcatag cattaaaaaa aatgcgaagt
     6181 gttctttgaa aaactgaaac atccaaaaag aatcaatcat taaatatcag caaccggatg
     6241 caaaggtaaa cttacggcat gcaaaaagga tgcactgatt atagacaacg ctatcttccg
     6301 gattggttgc gcaagcggcc aatgtgaagt ataacaaagt gtatagtttt acgatccctg
     6361 gcatgtggca atttccggag atatccgact ttcaaatgat acgtttctcc gaaacgcaac
     6421 tctacctgac gaaggcaggg acgccgggtt gccgaaacaa aaaatgcagc tacaggtatt
     6481 tcgataccag tacacatttt gaaagaggtg caagggcgaa agcagcaatg ttttcaagtc
     6541 atgttaccga aagtttcagg cagcgcagcg caaaagaatt aaaagtcaaa acaaggttcg
     6601 atatttaaaa aattgacaaa ggcgtttatc actaaacaaa tttatttttt ggtgaaaatg
     6661 ctgagagaag aattaactta tggacagcaa ggtaaaagcc ggttgcatac gggaagaacg
     6721 atggtaaaac atcgggaatc cgggcagcaa cgggatgaag ggcgaaagcc agccgtgagc
     6781 ttacgagtaa gtcggactcc gtactgcagg aaaaaatgga aatccccctc aaaaagggaa
     6841 agaaatcaat ccgggcagca tggaataatc atctgagcca atgactgatg gcaacatcag
     6901 gaagcggtaa agccgtaaaa agccgaagat gaaaaggagc actgaacgaa actgagtacg
     6961 aaaccctaac aggaagtaat acaaaagatt cagaagctgc gtaaaagcag ccgaaaaatt
     7021 cagaaatccg ttcggaatgc agataagcaa acgaaatcat ttcccgtaaa gcgcagcaga
     7081 ggaaactctg agacgaaata aagcgactga tatccgacac gtcaacaagt taatgatatg
     7141 agaattcaag atttgtggtg atttttactg taaatccctt caaaaaggat tatgagataa
     7201 gacacctgct tcaccccctt aaatgaaagc tgacgcgagc aatcgcaaag tttgaaactt
     7261 acccggtttc gggaattgca atcaggcaat tttgaaaaag attaccggca ataaaccgga
     7321 catggcgaag caaaaaacta aatcgacgaa aggtatggct gagatagtct gatgaagcag
     7381 ccgctgaaac caacccgtaa aaaggttggc aagcgatgct gaccgaagaa gacaacactg
     7441 gcacgaaagc gaagctaacc gcaagccgaa aaacatatag aaatcatact cgaagcaaca
     7501 atacagttat tcaaagatat tttacattac attttaatgt ttcaagaggg tgcccgacag
     7561 gacaccctct tactttttta aaatatcccc tgaaaacttt tgtgaggaat aattctgaaa
     7621 aaaaataccc aacgttttgc taagactgct cttttctcag ccaggctgaa ataattgtga
     7681 tttcgccctg cgaataatgc tctttcagtt tgctgtaaat ggatacatct tcgtccgttt
     7741 tttcaagaat ggatttagcc cgctgcaatt caccgggcgg aacaaactct tcggcagtga
     7801 gttctccttt ggtcacatac cggcctaaat gaccgtaaat ggtgccgacc gacaaactgc
     7861 gttcggcggc aatcttttca acacttttac cgcttttata ctcctgaaac gaaagcaatg
     7921 ccgaacttcc ttttattttc ttttcactct tctctttttt atctttcctt tttcgttttt
     7981 ctctgccggc aagctcgtcc atggccgaag tcaatccatt ttcaaggcaa taatcccgaa
     8041 tctcttcaag gaaaagttca ccgaattttt caaccctcgc ttctccaaaa ccgcttattt
     8101 tgagcagatc ggatttggtc tgaggaagat aatcggccat ctcggcgata gagtgagagc
     8161 ctgcaaccat ataaaccgga agtccggtat catctacaat agaatttctc aactcataca
     8221 aaatacccat cagatcggga cgacgggaag ataatttttc ctgtttctga tgacgcgaat
     8281 aggatgtgta tttgatggca ggcaattcaa atgattttct gagctgaaaa tacttttcga
     8341 taccaaatcc atccctgatt ccgctaatcc aatgtttttt ctgtgctgca aaagtaaata
     8401 tatccagata gtattggtcg aattcttttg cattctctct gctgtcggtt gtggcaggcg
     8461 attgtctgag cgtatcggtc agcaggttga gtttttccga gaaataagta tctgccgcct
     8521 ccagtctgag attcagcgca tcgtgattga attcattgtg ttttagaatt tgacttatct
     8581 gtgttccgaa acgtgctcca acatcgtgaa tagcatttat ttgtgcattt atatttctga
     8641 gaaactccag ggtttcgcta ccgaacgaag tttcggcatc ggcggtagat gtaagccatc
     8701 tgcccgatac ggctaccatc tgccgaaaat cgaaaacctg caacaggagt gacagaaaat
     8761 actcctgtcg cgatgatgag aaacggtttt ccagctcatc cacatttaaa tgccgcgacg
     8821 aaaacctcag aatttcgtta tcgttctgga tgctgcccgc attgattttg ctttgcagaa
     8881 ccagtccctc aagcgagcgg catctactca aagccacgta cacctgaccg gctgcaaacg
     8941 ccgattcagc gtcgatgact gctttatcaa aagtgagtcc ctgacttttg tgaatggtga
     9001 tagcccatgc cagcctcagc ggatactgac gaaaagtgcc aagcacatct tcctttacca
     9061 gaagtgtaga ctcgtcagtc tgataacgga tattctccca ctccaccggc aaaaccgcaa
     9121 tcggttcatt gtcttcggga cattttaccc ggatgatgtc tttgtcccat ccggtaatca
     9181 gcccgatttt accgttaaaa aaacgccttg gattttcact gtcgtttctc agaaacatga
     9241 cttttgctcc tattttcagc tccagtttgt catcggccgg aaaactacgt tccgggaaat
     9301 caccggtgac aactgcttcg aatatctttg taccagattt tatcttatcg agttcttcac
     9361 ggtttatccg gtcagctttg tagttgtgag tggtaagaat tatatacgta tcgtcggttg
     9421 cgggtacaaa aaacggattg tagcacttcc cgagcagtgc gagactctca gcggataacc
     9481 ggttgttgcg tacctcgttg agcaattgta tgaattcaat gtctttctgc ctgaaaattt
     9541 tctcgaattc gatgtgcaca aaaggttttt gctgaaacac gaggctgtta aaaaaatata
     9601 caccctggta aaatctcgaa agaatctgcc actcttcgtt ttgagctacg ggcgatagtt
     9661 ggtacatatc gccgataaaa atcatctgaa cgcctccgaa tggttcgttg tgtcggtatc
     9721 gcacgtgtcg gagcaccgta tcgatggcat ccagaatgtc agcccgcacc atgctaatct
     9781 cgtcgattac gagcagttcg agttcccgaa tcacttttcg ccggttggat tgcattttca
     9841 ttttgcctat taattctttt cttcccattt cggtagggat gaaaggtgtg agcggcaact
     9901 gaaagaaaga atgaatggta gtgccgcccg cattgatggc agccacaccg gtaggcgcta
     9961 ccacagctat ttgttttgga gaagtttcgc gcagacgatg caaaaaagtg gttttaccgg
    10021 ttccggcttt tcctgtaaga aaaacattct gattagtaag tgtggcaaac agcgaggcgt
    10081 attcgtgagc agagtatgtt tcaggcattt tcaggttttt aatttctttt ttcaatctta
    10141 gaattaacac acaaaaatag agaaaaatga ggatataaaa aaaccgttgc tttcatttca
    10201 gagcaacggt tattcattat ttattgattt atgatagcta catcattatc agaaagttga
    10261 gctaattcct ttcacttttg agaaagaaaa cgaggcaagc ttttctattt gttctgttcc
    10321 ttagcccagc tgtccttcag cgatactgtg cggttgaata ccagtttttc agcagtgctg
    10381 tccgggtcaa cattgaaata accgatgcgc tgaaactgga aacgatccag cggttgtgca
    10441 tctttcaggt attcttccac gtagcagttg gtttttactt ccagcgaacc ggcattaatc
    10501 agttcgcgga aatcacgctc gtcggccgat ggattttcaa cactgaacaa ccggtcgtac
    10561 aggcgtacct ctgcaggcag actgtgtgct gccgaaagcc agtgaagggt tcctttcacc
    10621 ttgcggttag catcgggcat accgcttttt gtcagcgggt cgtattcggc atatacttcg
    10681 gtaatgtttc cgtcttcgtc ttttttacat ccggtacatt ttacgatgta agccgatttc
    10741 agccggactt cctgtcccgg agtcatgcgg aaaaacttct taggtgcttc ttccatgaaa
    10801 tcctcgcgct ccatccagag ttcgcgtgtg aaagcaatgg tatgagtacc cgaattttca
    10861 tcttcgggat tatttaccgc ttccatcatt tcggtttttc cttccggata attggtgatg
    10921 ataagtttca ccgggttgat tacagcgctc actcgagtcg atgttttatt aagttcctcg
    10981 cgaacggcgt gttcgagcag tccgaggtca ataattcctt ctaccttggt gtagcctatt
    11041 ttgtcgatga aattgtgaat actttcggga gaataccctc tccggcgcag tccgcacaga
    11101 gtcggcatgc gcggatcgtc ccagccactc accaatcctt cctgaaccag ttgcagcatc
    11161 ttgcgtttgc tcatcacggt gtaagtgatg ttcagcctgt tgaattcaat ttgtttcggg
    11221 cggtattccg tttccttaaa ctggtcgata aaccagtcgt aaagcggacg gtgcactaca
    11281 aattcgagtg tacaaataga atgggtgact ccctcgaaat agtcggattg tccgtgggca
    11341 aaatcataca tcggatatac tttccaggtg taaccggtgc ggtgatgcgg gtgagtggtg
    11401 ataacccgat atagtatcgg atcgcgaaaa tgcatattag gcgaagccat atctattttg
    11461 gcacgcaata ccattttgcc atcgggtatt tcgcctgctg acattttcat gaacaaatcc
    11521 agattctcct gcaccgatct attccggtac gggctttcgg ttcctgctac agtgggagta
    11581 cctttttgct gagcaattat ttcagatgat tgttcatcca cataggcttt accttcgttg
    11641 ataaatctga cggccaaatc ccacagttgc tgaaaataat cggatgcata atacacatta
    11701 ttccacttgt aacccagcca cttgatgtct tccatgatgg aatccacata ttccacgtcc
    11761 tctttcaccg gattggtatc gtcaaatcgg agattacacg ttccgttgta tctttcggct
    11821 attccaaaat caatgcaaac agctttggca tgaccaatgt gcagataacc gttcggttca
    11881 ggcgggaaac gggtctgaat acgtccgtca ttcactcctg cggcaaggtc atcttctaca
    11941 atttgttcga taaaatttaa actcttttcc ataagcctct caaaatcctg cctgtggcag
    12001 gtaggctccc cgaaagggag acttgtttta tatcaattat tactaatatt ttctttcttt
    12061 ttgctatgca agtcctctgt tcgggaggaa cctggcaggt tttttttaat caaaatagcc
    12121 cttaataatg cctctcggag agtttttgat aaattgaagt atttcatcgc gttcattggt
    12181 agccggaagt tcagcctgaa cccgttcaac cgaatgggtg gtgttcattc ccgattgata
    12241 aatgaagcgg taaacatcct gaatatcgcg aatctgctcg ttggtaaagc ccctgcgacg
    12301 taatccgatg gaattgacac ctgcatagga cagcggatcg cggccggcag tgataaaggg
    12361 aggtacatct ttagtgactc tggatccacc ctgaatcatg gtatgagctc caatgcgact
    12421 aaactgatga atcatagtgc ctcctccgag caccgcataa tcgtcaattt ccacttcacc
    12481 ggcaagctgg caggcgttac tcataatgac attattgcct aacacacaat cgtgtgccac
    12541 atgacaataa gccatgagca ggcaattgtc gccgaccacc gtttttcctt tcgacacagt
    12601 gcctctgttc acagttacaa attcgcggat ggtagtattg tcgccaatta ttaccaggct
    12661 ttcttcgcct ttgtatttga gatcctgtgg aatacctccg ataacggcac ctggaaaaat
    12721 ccggcagttc ttgccgattc tcactccttc gagtatagtt acgttagagc cgattgtggt
    12781 tccttcacct atttctacgt ttttatctat tgttacgaat ggttcgatta cgaccgtacg
    12841 ggcaatttta gcatctggat gaatgtatgc aagtggttgt ttcataaaat tatttgagtg
    12901 ctttgaacct cgtaaaatga gactttaagt gcacttgtga gtattttaat atttaaattt
    12961 attcataaaa aaaacacttt tagaaaaaga gcaggaatca tatatccaac tgctattttt
    13021 ccttaagcaa aataaaggct atttattttt aattacctga gccataaact cggcctcggt
    13081 agcgattaca ccgcccacga atgcgtaacc tttcatcgta gctattcctc ttcgaatttc
    13141 gtccatcagc gatacgtgaa aaactacggt atcacccggc actactttct gccggaattt
    13201 cacattgtct attttcataa aataggtgga atacaactcg ggatcttcca gttgactcaa
    13261 cacgagcaaa cctcctgtct gtgccatggc ttcaatgagc agtactcccg gcatgaccgg
    13321 ttcgtcgggg aaatgaccca tgaaaacagt ttcgttggac gatatatttt taacgcctat
    13381 gatggaacgt tcgcgaattt cgagaatttt atcgaccatg agaaatggcc agcgatgcgg
    13441 cagcattttc cggatggcat tggaatcgag caaaggaggc accgtgtcat catacagagg
    13501 cgactgcact tcctgtcctt ttatttcttt gcgaatcagt tttgccactt cggtattcac
    13561 cttgtgtcca gggaaccggg caatgacttt tccatgaatc tgctttcccg ccaaagccag
    13621 gtcgccgata atatcgagaa gtttatgacg ggccggttcg tttttgaatt tcaaatcgcc
    13681 gctgataata cctaactcat cgactttcac ccgggggtgt ttcatcagat cggccagttt
    13741 gttcagttca tcctgaggca tttctttatc gtaaataacg attgcatttt ccaggtcgcc
    13801 gccctttatg agattctgac taagaagtgg ctctatttct cttacaaata caaatgttct
    13861 gcaaccggct atttcttttc caaaatcaga caaatcttcg agagtagcat actgcgccgg
    13921 caacacggga gaattgtagt caacgtaaac atccacactg aagtgatcgt cgggcagaat
    13981 agtgattttc gcattggttt ctacatcgtg ataatctatt cttttcttga cgatgtaaat
    14041 atctttttca gctttaagct gttcttctac tcccgctttt tcaatttctt cgtaataata
    14101 ccgggcactt ccgtcgagga ttggaaactc gggagcatcc acttctatca ggcagttgtc
    14161 aacctgccag gcaaaaagag ctgccatggc atgttcaatg gtgctaacct gcatttctcc
    14221 ccttttaagt atggtaccgc gattggtgga aaccacataa tctgccagcg ccgttatatc
    14281 gggctgtccg ggcaaatcgg tcctcctgat tctgattcca tgattcacgg gagcgggaag
    14341 aaaagtgata ttaatttcca ctccggtgtg aagtccttta cctttcagtc cgaaagctga
    14401 tttgattgtt ctttgtgttg ccatattttt atgatttgga tttagcagtc cgaagtttga
    14461 atattaaagt tatgagcttg ctgaatgaat ttaaattcat tcccgttgta attatttaaa
    14521 acaaaaaaaa cttctcttcc ggcaggaaac ctcttctttt tcaggttttt acttttttta
    14581 ctgaaagtat ttatccggtt tacttcattc ctgttcggat cttcaggttt tgagtttctt
    14641 gttcagtgct ttgtattgcc aagtacgagt ttcaggtttt ctatttgctt ctgcagttcg
    14701 ttaactgtgc gctgtaaatc gggcagattt ttataaacta cagatgccct gcgaaaggtg
    14761 ccgacgggta ctgccggata accctgcatg atgctgttgg gttccttcac gttgtttgga
    14821 acaccggtct gagctccgaa cacgcatccg tcggctattt cgatatgacc ggatatgcca
    14881 acctgacctg cgagaacaca tttttttcct attttggtag atccggctat tccggattgg
    14941 gctgcaatga cagtattttc tccgatttca acgttgtgag ctatttgtac cagattatcc
    15001 agcttcacac cccgtcggat gatggtgctt ccgagggtag cacggtcgat ggtagtattg
    15061 gcacccactt ctacgtcatc ttcgatcact acattaccta cctgaggaat ttttctgtat
    15121 gatccgtcgt ttgccggtgc aaaaccgaat ccgtccgatc cgactactgc tccggcctga
    15181 agaatacagt tttttccgat tatgcattcc ttttctaccc tcactccggc gtgcaattgg
    15241 gtgttttcac caatttttac attttcacct atatatcctg aagcatctat ggaagcatag
    15301 tttcctattt ccgccccttc acctacaaat acaaacggag ctatgtaaac gtttatccct
    15361 attttggctg aaggtgaaat gtgtgccagt gccgaaacac cgtttttctt tggtttcaac
    15421 tgctcgatgg atgataaaag cattgcaagc gattcgtagg cattatccac tttaataagc
    15481 gtggctttca cttccttttc gggttcgaaa tcgcggttaa ccagtattat actggcattg
    15541 ctctcgtata aataatgact atatttggga tttgacaaga agctgagcgt tccgttttca
    15601 ccttcttcta tctttgaaaa ttcacgaact tttacggccg gatcgcctat gatctcgccg
    15661 tgaaggtatt cagctatttg ctgtgcggtg tactccatat cttaaaaaaa tttgggtgca
    15721 aaattacaaa actattttca ataattattc tctgacctta ataaaagcca attatatctt
    15781 acagtagcac aaaaaatgtt tttctactgt tttagtcagt acctgaatat tcagcatatc
    15841 cgaagcatcg gcaatgtctt ttattgttcc atccttataa agaatactaa tactctcatc
    15901 ttctggactg tacgtatctg tagaaacgac ctcttcgccg atgaaataag cggcttcgta
    15961 agctgaaatt ccaaaatgtt cctgatattt tttcaggtat ttttcgcgca gttcatctcc
    16021 ctgttgtttg cctgtaagac tttctacctt gaagagtttt cggttggcaa actggctgca
    16081 gagtgcagca agcacagtgt ctttacactg ttcgctccat actttgatgg cgcagagcac
    16141 atcggcatca tccagcatag caaaatgatc cagcgtttcg gccgtatgaa tgaagtcgtt
    16201 ccggcttata ttattgtaaa gaaaatactt caatgccggc gaaccaaaaa gttctactcc
    16261 ctgcgatgca atttcttttg ctcttttcag aatatttatc agcatttttt cggctgccac
    16321 cgaggtttta tgcagatata cctgccagta catcaggcgg cgggcgacca gaaatttctc
    16381 aatggaataa atacctttgg cttccaccac cagcttgtcg ttgtgtagat tcagcatttt
    16441 gatgatgcgc gaagctccga taatcccttc gctcacgcca cagaaaaaac tgtcgcggct
    16501 caggtagtcg agcctgtcca tatccaactg gctggcaacg agttgatgca gaaaatgttt
    16561 cggatactga ttggtgaaaa tctgcagggc tgtttcgagc cggccatcga aatcgtcatt
    16621 aatccgctgc atcatccaca gcgagatatc ttcgtgtgat gttccctgta caatgctatt
    16681 ctcgagcgtg tgcgaaaagg gtccgtgacc gatatcgtga agcagcatgc aggctctggc
    16741 tgcatcttct tcggtggcac tgatattctg cccggtaagc ctgagctggg ctattgcctc
    16801 acccatcagg tgcatggcac ccagcgaatg aagcatgcgg gtatgctgtg ctccgggata
    16861 tacgaaggat gacaatccca actgtttgat tcggttgagc cgctggaaat aacggtgttg
    16921 aatgatatca tacagaaaat cgttcgggat attgataaat ccaaacaccg gatcattgat
    16981 aatttttcgc ttcataggcc ccctaacctc ctaaaggggg aatttggtaa gttgaaatga
    17041 ctttttatta caaatatatt gaatacaaat ttttaattat agcttgaagt ctgaaacacg
    17101 aaacccgaat tttaagaaaa caaattactg ttcgaaactg cattttgacc ggtattattt
    17161 cttgcaatat cgccgggaag caacgttttt tttaagaaca ataaattatg cgaaaaaaca
    17221 caaattatga aagaaattct tgttcggaaa aaacggtgct aatcagtgtg atttttcttc
    17281 aggcaaatca gaataaaatt agtttcgggc tcagcgaacc cgaaactcag aactaataag
    17341 ccagtatatc tgagagccac tcccaagctc ttccgactgc cggtatatgc agtcgttcgt
    17401 cgggtgaatg aactccccgc atggttgggc cgatagaaat catatccaga tgcgggtatt
    17461 tcttcagaaa gagaccgcat tccagtccgg catgaatggc tttcactttt ggctcggttt
    17521 tgtagagttt tttgtagctt ttttcggcaa ctttcagtat ttctgaattc aggtttggtt
    17581 tccagcccgg atagccgtcg ccgtgagtta ccttgcatcc ggccagtttc agtacagctt
    17641 ccacctgatg tgcaatgtcg tatttggccg attctactga acttcgctgg ctagtggtga
    17701 taataatctg ttctccttct ttcattttca ccgaagcaag gttggtggat gtttccacca
    17761 gtccgggcat atccatactc atttttttta tgccatgagg acacgcgtag agtgcattca
    17821 gcagttttct ggtacttttt ttagtcaggg tttcttttgg agtttcatcc gaatgaagtt
    17881 ctatacgcat tttggtttca atattctgaa attcctcttc aatatctgaa actacccggt
    17941 tgaaaataac ccgtacattt tccttttcct tgtaaggcac acaagctgtg gctgaagctt
    18001 cgcgcggtat ggcatttcgg agatttccac catcgatttt aactaaacga agatccatat
    18061 cccggagcat attccacaaa atccgattca gtaatttatt ggcattggca cggtgtttat
    18121 caatatcgtc gccggaatgg ccacctgaaa gtcctgaaac cgagaccgaa aatgcaaaat
    18181 atcctttagg agttttcttc gtttcgtaat tgaaagtagc tgttgtatca attcctccgg
    18241 cacaacctac gaatatctcg ccgtcatctt ccgaatcaag attgatgagc acatcgccgg
    18301 tgagcatatc gttgctcaac gcaaatgcac cggtcaggcc ggtttcttcg tcggtagtga
    18361 agaggcattc gaggggagga tgtttgatat tatcggatgc aagcacacca agcatcaaag
    18421 ccatgccgat tccgttgtcg gcgcccagcg tggtaccttt tgcccgtagc cattcaccgt
    18481 caatttcggt ctgtatcgga tctttttcaa aatcatgttc tacgttactg tttttttcgc
    18541 acaccatatc catgtgcgat tgcagaatta cggttttttt attttcatat cccggagtgg
    18601 ccggttttgt aattaacaca ttacctgctt cgtccgttcg ggtgggcagg ttgttttttt
    18661 taccaaattc ctgcaggtaa gctcttattt tttcttcttt tttggatgga cgcggaatct
    18721 gagtgatttc gtagaaatga ttccaaacga tttgcggttg taagtctttc atatatatgt
    18781 tacgttttaa aataatttat tactttgaaa aaaagaaatt actgataaat gttacgtgaa
    18841 aaactttttt tcatcatcgt aatatttaac actcgaagct acaaatataa acaaaagaaa
    18901 cgtaactaag gttttttaca agttgcaaaa agtgtcattt gttaaataac tgaatatttt
    18961 ttgcatttta tctccaaatt agcttcattt tgtttccaat cgggtttaca tttacaccaa
    19021 aaagggataa aatatgtgca tttaagcttt tttatatcta aaggttagcg ataaagtctt
    19081 acttttgtag tcaatatgtc ataagttgtt aaccttattg aaataccttt gaagaacttc
    19141 ttacaaatca ttaaagattt cgataccatt actttacctg aaatgggtaa agtaatgctg
    19201 ctcaaccgta cagatgtaaa attttctttc catcgcgatt tgttatctga tattctgaca
    19261 cttgccaaag ccgattacct ttctctggta atcaacgaac aacattacgc cgactacgaa
    19321 acacattatt ttgatactcc ggaatacggt ttctatcttg atcatcataa taaacggctg
    19381 aatcggtata aagtgaggac aagaagttac gtgaattcgg atttgcattt ttttgagata
    19441 aaattcaaat caaataaagg ccgtaccatt aagagccgta tgaaaatagc acaacccgat
    19501 tcgtgtatta cggaagaatg cgctgaattt ctgaaaacga aagccggtat ttcggccgaa
    19561 aacttaaaaa gaagcattca ggtaaactac aaacgaatta cgctgataaa caagggaatg
    19621 accgaacgaa ttaccatcga ttttgacatg agttatttga tcgatggagc caggtacgaa
    19681 tatcccgaac tgataattct ggaagtgaaa caaaacaaat caagccaatc ccggtttatg
    19741 gaaatcatgc atgagcagca cctgcatcga gtatcgctta gcaaatactg cttcggtatt
    19801 gccagctacg tggatggaat taaaaagaat tactttaaaa atcaaatacg ttatgttaaa
    19861 aaaatttgca acacagcatt caattaaatt cattgtactt ctttcatttt ttgtaatcat
    19921 tcctttttct cctctaccgg ctcaggacga actgatgatg gaagaaacag gccaaatgtt
    19981 caacgaggaa ccggtagcat cactcagccc cgacagtgtg ctgagagctg aattcaaagc
    20041 cgtaatcaaa ccacccaaaa aagaggaagt agagcttgga ttaacttttt ttctgagttt
    20101 gttgctggat ttactcatgg tttttctcat tatccgaacg atatattacc ccaattaccg
    20161 taaactggac acagtattca ccttcgtgat gttcaatgtg tctatttttc tgctcatttc
    20221 actgctcaat cagataaaaa tatcgatggg tgcggcattc ggtctttttg ccgtattcac
    20281 catgctccga tatcgcactg caggcatcag tatcaaggat atgacttatt tgtttatatt
    20341 tattgcgttg ggtttgatca gcggagtgcg actacagatt catgatttgc tcattatcta
    20401 cgcagttatc tatgtgatgc ttttcttact ggataccagg tatttcatca aacgcgaatc
    20461 taccaaaacc atattttttg acaacattga ccttataaat gcttcgaaag aaaaagaact
    20521 gatcgaagaa ttaaaacttc gtaccgggct aaaaatccat cgtgtatcca tcgaagacat
    20581 ttcatacctc agagacagcg ctaccataac catttattac tacgactaat gcgtaaaata
    20641 acattcgtac tgtttctttt agcgttcctt accggtgtaa aaacaatggc tcaggctcag
    20701 tacggcacat ggacgagcgt tggtgtagag aaaaaactgg gtaagtggaa actggaagcc
    20761 gataccgaaa tccgcaccat atactacctc aggctgataa accggtggag cattggactg
    20821 gatgctgaat ataaaattgc aaagccactt gaagtaggtt tgagctacac gttgatgaac
    20881 gtactcgacc agaaatacct gaattaccag ttcagaaaca ggttcaacgc gggtatcacc
    20941 ggaaagcaaa aatggggaag atttgatttt tccctgtcag aaggtcttca gctgacgaca
    21001 aaaaatgcca gcaaaagaat tgacgattac ggcaatacag atacgtataa gataaatcct
    21061 gccctgatgt ggaaaaattc aatgcaggca gaatacgaca taccgaaaag caaactcaca
    21121 cccggtggtg aaatagaaag ttactacgag ctcaacaatc cggatgggaa caaatttgac
    21181 aaacttcggt attcgctatt cttaaaatat aaaataaaca aacacaactc cctgaaactt
    21241 gccggaatct acaacgacga actgggctcc gacgatgccg attactccgg taaatatatt
    21301 gtggatctca aattttctca cacattaaaa taactgatta tgaataaatt actcaaacaa
    21361 ctgttcatgc tgaccgctat tgcagtcatc gtggcaggat gcgatcctga aaacgtaatc
    21421 ggaaaagata ccaccaaatc agaaactgcc accgattacg agtgggacaa cagcaatgta
    21481 aaaaccatcg ttttcaacca aaacgccatt acatccactt caaaagacgt tttgattcaa
    21541 ggaacaacag caaccatcac ttcggccggt tattacaaca tcagcggcaa cctttctgac
    21601 ggacagcttg tagtgaatgc ccccaaagcc aaactaaaaa taatgctcag cggtgttacg
    21661 atggcaaatt ccactacatc tccattcttt atccaggctg cctccaaagt gatagttttc
    21721 ctcaaatcag gaacggtaaa cacaatatcc gacccggcca tctataccaa tacaggcgaa
    21781 ccgcaggctg cattattcag caatgcctac ctcgctttca cgggcgaagg aaccttaaat
    21841 gtaaacggaa atcacaacga cgccatctcc agcgacgatg aagtgatcat caacagcggc
    21901 aatttcaaca tacaagcccc ggacgatggc atacggggta aaaactatgt gcttatccat
    21961 gattgtaatc tggtagctgc agtcaataca ggacatgcgc tgaaatcgga taacaccgta
    22021 accgacggac gtggttatgt gaaaattgac ggcggaaata tttcgctcac ttcagctcag
    22081 ggcgatggga ttaataccac gaaaagaatt ctgatagaaa acggaaaact gaccctcacc
    22141 gcttacgaga gtcagggttt gcgaagcgat tctttggttc atatttcagg aggtgaaata
    22201 accgtgatga aaaactccag gcaaggcata aaaagtccct acataaagct aacgggagga
    22261 acaacacaga tcacatccaa taaaatagcc gtaaatgcca ccttcgaaac tgctgctaca
    22321 cagcccgaca gcagcctgtt gaccgtatcg ggaggtaatc tgtatatcaa cacgttgagc
    22381 cacgggcttg tgagcgacgg aaatattcac atcgacggag gcacaaccgt agttcagggt
    22441 ccctacgata attcaaaagt ggcggtatcc actctaggcg aactgaagat tacgaacgga
    22501 atgctggtag caagcggacc gaatgcaggc tctttgatca aaagtccgga caacacatcc
    22561 actcaaaaca gtctgaaaat aacatccgga actctgggaa ccaatctgat aaacattcag
    22621 gatgccggcg gtaactcact tgtgactttc aagcccatca aaagcgccta ttacctgctg
    22681 ttttcatcgc cggcattgaa aaccggaact accttttcgg tttttacagg cggcagttat
    22741 tcgggtggaa ctactattaa cggtttttct accggtggta tttacactcc cggaacaaaa
    22801 aaaagcacgt ttaccctatc agaaaaagta acaccggtta atttctaatt cagtcggtaa
    22861 gtcccggaaa ttgttattga taaaatggat tgattaagta ttcattttgc gaataaatta
    22921 atcgtttgct tcaactaaat ctgataagta aatgttttgc ctgttgcttt tttattcttt
    22981 tctttgtaag taaaaatata caacaatgct tacatgaact ggactaaaaa gcaaacttcc
    23041 acgctggcag tagtagccat aacttcgttt atgggtacat ttcttatctc ttccatcaat
    23101 atcgccctgc cgtctatcga aaagacattc gggctgaatg ccatcatgct gagctgggta
    23161 gtaacttcgt ttctgctggc aacagccatg tttctgcttc ctgcaggacg ctggagcgat
    23221 atttcgggcg tggtcaggct ttataaagcc ggactgatta tttttacatt atcatcgctt
    23281 ctgtgtggca ttgctccgtc gggtggctgg cttattgcag cccgttacct ccagggtatt
    23341 ggcgctgcat ttaccaatac caccgggcag gcaatactcg tgggaagttt tccaccccaa
    23401 aaccgcggca gcgtattggg aatatcggtt tcatcggtat atctcgggct ttcgttcgga
    23461 ccatttgtgg gaggattact cactcagcaa attggctggc gctccatttt catggcagca
    23521 gcccttatcg gactcgtttc aacggtcgta tcgtttcttt atttaggtaa agacgaacaa
    23581 accgggcaga caaaacagaa aatggggcta aagggtgtta tcttttttat gctcggactg
    23641 gtttgtatgg tgtatggatc atcgctcatt cctgccacat caggctggct gatcatggcg
    23701 gcaggacttg cagcactgat attattctgg aaaatagaaa gtaaatccaa aatgccggtt
    23761 ttcgacaccg gacttttcac caaaaaccgg atgtttgcgt tttcgaacct ggctgccctg
    23821 attaattacg gatcgacttc tgccattgtt ttctttctga gtctttatct tcagaaaatt
    23881 caacatctta cccctcagca agcaggtctg attctgatag cccaaccgat catgatgacc
    23941 ttgttttcgc cattggtagg ccggctttcc gaccgtcatc aaccccgtta ttttgccact
    24001 tcgggcatgg ctatgtgttc tgtcggattg gcttcgatgt catttctgaa cgaggtcaca
    24061 ccccactggg taatcatttc gatacttatt tggataggaa tcggatttgc actcttctca
    24121 tcacccaata tgaaccttat catgagttca gtaaaccgaa ctcaatacgg acttgcctca
    24181 ggtacagccg catccatgcg tgtaatcggt cagataacaa gtatgacaat cgtaacactt
    24241 ttatttgcag gacttttcag cggaaaatcg atcgaaaaag tttctgattc attgtttctt
    24301 acagccatga aatggggttt tatcaccttc tcactcacaa gtctggttgg catttatttt
    24361 tcgtatgtca ggggaaatgt aaagcgaaaa actcctctgc ctgccgattc acaggaataa
    24421 tttttcaaaa aaaaatgttt tctgaaaatg cttatatttg tatcatcgaa caataatata
    24481 ataaaaacta aacctaaaat cagaaatgat aacaatcttc atcattgcgc tcacagcact
    24541 tatctcctac ctggcgttca acaacagctc gtacatgaac aagctgatat tctatccccc
    24601 cgccgtgaaa agaggaggat ggtatcggct cgtcacttac ggtgttttac acgcagattt
    24661 tacccatctg attttcaata tgttgacatt atatattttt gggagttata tcgaaaaatt
    24721 ctttagagca atattgggag atcaagcggg tggattctgg tatatcataa tgtacatcag
    24781 tgcactgatt atttccattc tgccaaccta cctgaaagaa aaaaacaatg ataactaccg
    24841 gggactgggt gcatccggag ctgtttctgc cgtggtattt gcctacattc ttattaatcc
    24901 catgagtttt atgggcgtca tgtttatccc cgtgatgttg cctgcctttc tgttcggcat
    24961 tttgtttgta gttctttcag tcgttcttca gaaaaaacat tctggaggta tcaatcattc
    25021 ggcacacata gcaggcggaa tttttggaat ggtattcatt gtcattcttt ttgccctgct
    25081 ctcagatatt aatattatcc gtcattttgc cgaccagatt cagatttcat ccatcagcga
    25141 tttgatacac atcggatatt gacttacctg aaaatattaa taaacgaaaa aaccggtgtt
    25201 cataccggtt ttttcttgct gatgtttatt tttgctggcc aaattccaga tatagagtga
    25261 tatgcacatc tttaccaacc gatttcagtt ccgggtcata cgccactccg taatcaaatc
    25321 ggtttatggt tgttttagct gaaaagccat aaatatcacc gccgttttta cctttcacgg
    25381 gaccattcat cactacatcg aatacaactg ggcgtacaac atctttaatt gtcagatttc
    25441 cgttcagttt gtaaatttta cctgattttt tgattgatgt acttgtaaat ttcattgaag
    25501 gaaatttggg gacatcaaaa aaatcggcac ttcgaaggtg tttgtcgcgc atctcaaccc
    25561 cggtatttat actgttaaca tccactgtga agtcaattct ggcattcttc acatcagagc
    25621 cttcaatcat tccatcaaat ttctcaaatt ttccggttac aaaactaact cccaagtgct
    25681 taaccttaaa attaatggaa ctgtggtatg gatctacttt gtaaattgtc tgtgcagata
    25741 aacctacaac aaaaagtgct gctaaaagca ctgtaaaaaa tcgtttcata tttctaattt
    25801 ttattcgtta tattgtgaaa aacattgtaa gtgtaatatt tgtttaataa attaatttta
    25861 taaagcatca acgataccat ataatcattt ctgaaatgat tttaattata tcctcaacgg
    25921 actttgaatg tacattttaa ttttaaaaaa atgatgcttg aaattcggca catattttgt
    25981 tttaacataa tcaacaattt cataacagaa atttttataa caacaaacaa cataagccat
    26041 gaaaaaacaa gtacttaaat caattgcagc agcaacaatt ttgttagccg ctttcacatt
    26101 caccggatgt ggaacctatt acggagcaac cgaccaggga ggttactatg atgattattc
    26161 atacaacagt ccgacctggg gaccttctta ctatgccgga gcacgttatt attattttcc
    26221 ggacattgaa gcttattatg atctggcaag ccgtgatttc attttcctga acaacgggca
    26281 gtggattttt gtacagaccc tttcgccgtt ctattccagc tttgatctgt attccagctt
    26341 catcatcatt atcaacagag gcgtttacca accctggatg caccatcatt attacaattc
    26401 acactatccc agatactatt acatcgatta ctatgactac agcaacatac cttacgtaag
    26461 aggtttcaac gaaaatcaga aaggagctat atactggcct gaaaaccaga gagaaagggc
    26521 aagagcctgg gacgacagaa acatccggaa caaccgccgt ttctcctact ccgaagccga
    26581 cagacgcgta caacaggaaa caacgagaca aatgtcaact caaaggactt caagaaccga
    26641 cattggccga agcaacactg ccgagcgtac gagaactacc gatgctgacc gcagccgtac
    26701 agagaccaga acaagcgacc aaaccagaac acaaaccagg acaaccaccg atcagaccag
    26761 aagtgccacc cgaactgata tacccgaaac aacgaggagc aacacctcta caacgagaac
    26821 agagcggact acagcatcac cgaggactga cacaggtacc aatacccgat cggatgcaca
    26881 aacttcaaca agaagaacta ctgatacaaa ttattacggc gaaccaatcg gacgtccggt
    26941 gagagtggaa agacagatgc gaagtactga aacaaccaca acaagaagca gaagtacaga
    27001 tacaaatacc gacacaaata ccgaaagaag cagctcatcg cgcacttccg gccgaaggta
    27061 aacttacggt ctctacatat atttactaaa gcggaatcta cggatttcgc tttttttttg
    27121 taaaataaat catcaacaca gcaggccata acccaaacat ttctgactta gggagaaagc
    27181 atttaacatt tcactaaatt tcgatttatt ttttgtacct ttgtatccga aaataaaata
    27241 gtctaaaaac aatttttaat gtaatttttt atgaatgtag ttacaaaaac catcacaatg
    27301 ggtgatggaa gagaaattac catcgaaaca ggcaaactgg ctaagcaagc cgatggttct
    27361 gtggtagttc gcatgggcaa caccatgctc ctggctaccg tatgtgcagc aaaagatgca
    27421 acacccggta ccgattttat gccactctct gtggaatata aagagaaata cggtgctgcc
    27481 ggccgtttcc ccggcggatt cacacgtcgc gaaggccgtg cttcggatta tgaaattctg
    27541 attgcccgcc tgattgaccg cgctctccgt ccgcttttcc ccgatgattt tcatgctgaa
    27601 gtatttgtga atgtaaatct tatttcggcc gacggagtgg atatgcccga cggactggcc
    27661 ggactggctg cttctgctgc tctggcagta tcagatatac ctttccacgg accaatttca
    27721 gaagttagaa taggccgagt gaatggcgaa tacctggtaa atcctacttt tgcccaaaaa
    27781 gaagaagccg acctcgacat tatcgttgca gcaactgccg acaatatcat gatggtagaa
    27841 ggcgagatga aagaagttag cgaagctgaa ttgcttgaag ccatccgcat agctcacgaa
    27901 gctatcaaaa tacaatgcca ggctcaggta gagttgatgg aagaagttgg aaaaaccgaa
    27961 aaacggacat acagtcacga agaaaacgac gaagaactca agcaagatat ccatacaaaa
    28021 acttacgata aagtgtatgc agtagccgca tcatgcaacg ctgacaaaca ctcacgcatt
    28081 gaaaactttg aagccgtgaa ggcagaatat attgcctcac tggagcccga agtagccgaa
    28141 gaaaagaaag atttaatcaa ccgatactat catgctgtgg aaaaagaagc catgcgccgt
    28201 tcgattctgg atgaaggaaa acgcctggac ggacgtaaaa ccacagaaat tcgtcacatc
    28261 tggagcgaaa ttgattacct gccgggagca cacggatcgg ctgtatttac ccgtggcgaa
    28321 acccaatcac ttactactgt aacattggga accaaaatgg atgaaaaaat cattgacgaa
    28381 gcactgatca aaagtaaaga ccgctttctg ctgcactaca attttccacc attctcaacc
    28441 ggtgaagcca aaccacagcg tggtgtcggt cgccgggaaa taggacacgg aaacctggct
    28501 tatcgcgccc tgaaacccat gataccaacc gattaccctt atgttgtacg tgttgtatcc
    28561 gacatactgg agtcaaacgg atcctcatcc atggcaaccg tatgtgcagg tacactggcc
    28621 ctgatggatg ccggagttca gatcaaaaaa ccagtttcgg gtattgccat gggattaatc
    28681 tctgaaaata aaggtcagaa ttacgccatt ctttcagata ttctgggcga cgaagatcac
    28741 ctgggcgata tggacttcaa agtgaccgga acccgcgatg gaattactgc cacacagatg
    28801 gacatcaaag tggacggact ttcatttgaa atccttgaaa atgccctgaa tcaggcaaaa
    28861 gccggacgta tgcatatcct tgataaaatt caggaaacca tttccgaacc acgcgccgac
    28921 ctcaaaccac acgctccccg catcgtggtg atgtttatac caaaagattt gattggtgcc
    28981 gtaataggcc cgggtggaaa aatcattcag aacattcagg ccgaaaccgg tgctgtagtc
    29041 tcgattgaag aaatcggcga gcaaggccgt gtagaaatcg cttcagccaa caaggaatca
    29101 ctggatgcag ccgttgccaa aatccgcggt atcgtggcca ttccggaagt gggcgaaaca
    29161 tacaatgcca aagtgaaatc aatcatgccc tacggtgcat ttgttgaatt catgcccgga
    29221 aaagaaggac tgcttcacat ctctgaactg aaatgggaac gggtggaaac catggatcag
    29281 gccggtttga aagagggtga aatgattgaa gtgaaattgc ttgacatcga cgaaaaaacc
    29341 ggcaaattca aactttcgca caaagcactt ttgccccgtc cgccgagaga agaaagacca
    29401 ccgcgtgaac caagaggtta gtaagatatc cgctcataaa acctaaagcc tcccggcatt
    29461 aggaagatta taaaaaaaca ctgctacggt actgaaactg tagcagtgtt ttttttggga
    29521 ttaagtggtt ggaacaacct aacgatgata cctgccacaa aaatgtagca acacccgtta
    29581 tcacccgtaa atgattttac ctttttcgtc tttgaattcc ttgaatttaa ggtctgactc
    29641 cggacagtcg aaacttttca gatactgttc catagcgcga tagctcatac ccatacttga
    29701 gaaacctccg tcgtgaaaaa ggttctgcat ggtcactttg cgggtcagat cactgaacat
    29761 aaccacacaa taatcggcac aatcatcggc tgtagcattg ccgagcggag ccactctttc
    29821 cgaaaaatca accaaactgt cgaaaccttt cactccgctt ccggcagtag tcatggtagg
    29881 cgactgggat atggtattga cgcgcacatt gtgttcgcgt ccgtagatgt atccaaagct
    29941 gcgtgcaata ctttcaagca ttgccttggc atcggccatg tcgttgtatc cgtaaatggt
    30001 acgctgagca gccatatacg acaatgcaac gatagaacct ccgtcggcga tggcttccat
    30061 ttttttggct acctgaagca ttttatgaaa tgagattgct gaaatatcca gtgttgttga
    30121 aagcatttcg taatccaaat cgtcgtaggt gcgttttttt cttacattcg gcgacatacc
    30181 gatggaatga agcacaaaat caatctttcc acccaacgct tccatcgatt ctttgaaaac
    30241 attttcaaga tcttcaacag atgtagcatc tgccggtatt aattttgcgt tcagtttgtc
    30301 ggccagttcc tgtgtggttc ccattctcac tgcaagcggg gtattggata gtgtgatgat
    30361 tgcaccttct tctaccgcac gctctgccac tttccaggct attgacatat tattcagtgc
    30421 accaaacaca atgcctcttt ttccttttaa taagttatga ctcatatttt tctgtttttt
    30481 agttgtttct gtatattaac atttcctttc ggaaatagtt ctttcaattc tttcttttcg
    30541 gcacaaaatt actaaaattg tgcataagca caaaataaat tgccggatta atccctgttt
    30601 tgtaaagcag agtttgcaaa tcaacttatt tttagaatca acaggtctat tttgtaatat
    30661 tctgaaataa tttgattata gtttttgtat ttttgtggaa aatcagacaa atcaatttta
    30721 taaatttaat gctatatttt catcatgaat tacgacgaca atgatccatt ttcgtccggt
    30781 aatcattacc ggcagatgcc tgtttttaag aagggccagg aaatagtaga tttaatcatg
    30841 cagattgcag cattaatacc tgaggacgac gaaatgttgc aagaggtaat gggcatgatt
    30901 ttgggtgatg cctatatgct gactgccaaa attgccaatg cagaaggcag cgatttatac
    30961 gaccataaaa tgcagaatgc agctattatt cgcaaagcgg cgatggattt atttgtgagt
    31021 tatcattcac tcaagatgca cggattcgaa catgcggaat actacatgat ggtaagggat
    31081 ttgattgagg agtaccggtt gctttttgtg gagtgggttg atggttttga taagagttgt
    31141 tacctcgtcg atcgttggaa tgtattcaat cctcccggag tacattatac cgatacagat
    31201 ccggatatag atcttggttt tgatcctgaa gattttgagg aggatgacga tttttaatgc
    31261 tgaaaatacg cgaaaacggc tgtcggaatt atctgacagc cgttttttaa ttgaacacgt
    31321 tctataacaa gtgaatagaa tttgattatt cctcttcttt tttctgtgct tcgtattctt
    31381 tcagcaattt gtcctgaacg tcagatggaa caagttcgta gctggcaaat ttcatgttga
    31441 acgagccgcg tccaccggta atagaactca gcgaagtgga atatgatccc atttccttca
    31501 atggaacacg agctttcagt ttttcaaaac ctttttcgct ttccataccc ataatcattc
    31561 cgcgtcgtcc ctgcagatcg cccattacat cgcccatgta ttccgatgga actgaaacga
    31621 caacatcgta aattggttcg agaatttttg gacctgcttc tttgaaagct gtgctgaatg
    31681 cattgcgtcc ggcaagcatg aaagagattt cattggaatc aaccgggtgc atcttaccat
    31741 cgtacacaat cacacgcacg tcgcgcgcat acgaacctgt aagtggtccc tgttccatac
    31801 gggccataat tcctttgaga atagcaggta agaatcgtgt atcaatggca ccacctacta
    31861 cgctgttgat gaataccagt tttcctcccc agtcgagtgg aatttcctgt gtgtcgcggt
    31921 tgcttatttt gtattcctgg ttgccaaaac ggaaaacgtc cggagcaggc attccctcga
    31981 aataaggttc aactatcagg tgtacctcac caaactgacc tgcgccaccc gattgttttt
    32041 tatgacgata atcggctctt gaggcttttg taattgtttc cctgtacgga atttttggtt
    32101 cgatgaattc gatttcgatt ttatcgttgt tttcaattct ccatttcagt gttttcaggt
    32161 ggaattctcc ctggccggaa actatcgttt gctttaattc ttttgaaacc tctaccaccc
    32221 aggtaggatc ttcttccttc atgcgcgaaa gtgcttcgct cattttttcc gaatttgatt
    32281 cgcttaacgg acggatagct cgacgatatc ttggatccgg atatttgata aaatcaaact
    32341 tgttttcggc atctttggag tttaaagtat ttccggtgcg tacatctttc agtttcagag
    32401 ctgcccctat gcttcctgct tcaagcactt cagccttatt gcgtatctga ccggcaacag
    32461 tctgtatttg cgacaatcgt tcttttgtac cccgattcgc atttacaagg tcatcacctt
    32521 ccttcagttt tccggacatt actttgaaat aggatacttc gccgatatga ggttcaacag
    32581 tagttttgaa gaaaaacaaa ctggtcggtt ttccggaatc cggttttact tcttcaccat
    32641 cggcattctg cggagcaggc atttcatcgg gtgatggagc tgccacactc aggaaattca
    32701 tgatgcggtg tacacacata ttcttttcgc ctgaagcaca aaataccgga aataacgaac
    32761 gtgaaatcat acctttgtga attccgtcac gcatttcttc ttcactgagc gtaccctgat
    32821 cgaaaaattt ctccatcaac gaatcatcgt tttcggcagc tgcctcaagc aactgttgaa
    32881 aaacatctgc agctttcgct ttttcactat caggaatttc gagtacatca ggagcagtag
    32941 ctcccggttt ccatttatac atcttttgtt tcaacacatc tattacagca tcaaaagagg
    33001 ctccggaact tacagcatat tgtaccggaa ttactttact tccgtacatt tccttcaatc
    33061 ctgtcagcgt gttttcataa tctgcttttt catgatcaag ctgattaatg acaaaaacta
    33121 ccggtttatg cagttcttcg gtatagcgga actgattcat ggttccaact tccacaccgc
    33181 ttgttgcatc aataagcatc agggccatat ccgtaacgtt aagagcagat attactccac
    33241 ctataaagtc gtcggaaccc ggacaatcaa tgacgttcat cattttattc ttccattcgt
    33301 acgacagtac tgttgagaaa acagaataac catattcttt ttctacagga aaataatcag
    33361 atactgtact ttgattctta actgaacctc tgcgttttat tacaccactc tcgaaaagca
    33421 tcgcttctgc aagagtggtt ttcccggcgc cggaactacc cagcaatgaa atatttttta
    33481 tttcatttgt ttgatatact ttcatattgc gttaatttat aaatagttat gtttcaatga
    33541 ttagaattta atagctcaaa aaattgatac gcaatttaca aaaaacttaa ttaattcttg
    33601 catcaaattt atatgttata aaacataata ttcaacataa tgaatcgaaa acatataata
    33661 aattctataa ttgctataga tatgaaaatt tttgagaaaa aaggagttat ttgttgtttt
    33721 aaagatttat aaatcggtga attcagagca ttagcaaaaa aaaatgtttc atacaatttc
    33781 atacaaagaa cttttccctg aaaaacggca gctatcggat tttatcccga tttatcggga
    33841 ctcccacgtt gtcttgcagt actcc
//
LOCUS       DAORKP010000004        30312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_27_length_30312_cov_10.117031, whole genome shotgun sequence.
ACCESSION   DAORKP010000004 DAORKP010000000
VERSION     DAORKP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_30312_cov_10.117031"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1941)
                     /gene="uvrC"
                     /locus_tag="PLN75_00525"
     CDS             complement(124..1941)
                     /gene="uvrC"
                     /locus_tag="PLN75_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRG02630.1"
                     /translation="MTKKQSLKNQIDLLPETPGVYQYFNEKDEIIYVGKAKNLKRRVS
                     SYFNKKHDIAKLNVLVKNISYLRFILVDTPEDALLLENNLIKEYMPRYNAMMKDGKTY
                     PSICITKEEFPRIFKTRNIDKRIGEFYGPFSSVWTINAILDIIHQLYKIRTCSLPLAE
                     TKISEGKFKLCLEYHIKKCKGPCEGLQSKEDYNQDIDEIRQIIKGNANEISKMLLEKM
                     KKLAAKFRFEEANEIKQKYDLIENYKAKSIVANSVVNNTDVFGYEEDESSAYVNILRI
                     SKGSIIQGYTIEYRKQLDETKEDILATAIVELRERLESTSKEIILPFPVDYNISGVEF
                     IFPERGDRKKLLDLSLQNVKQYKLEKLKQAEKLNPDQKAIRLLTEMQEKLQLDKLPMT
                     IEAFDNSNISGSDAVAACVVFKKGKPSKKDYRKYTIKTVEGPDDYASMKEVVRRRYSR
                     MIEEESEFPDLIVADGGVGQMEAIRKVVEDELNLKIPIVGLAKNTKHKTRELLFGFPP
                     KEIGMKPNETLFILMANIQEEVHRFAIKFHREKRSKTQTASELDKIPGIGEKTKNELI
                     AHFKSLKRIKTAEFSDIENIIGTHRASKVYSHFNPNLKA"
     gene            complement(2187..4061)
                     /gene="mnmG"
                     /locus_tag="PLN75_00530"
     CDS             complement(2187..4061)
                     /gene="mnmG"
                     /locus_tag="PLN75_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRG02631.1"
                     /translation="MDFKYDIIVIGAGHAGCEAAAAAANLGSKTLLITMDMNKIAQMS
                     CNPAVGGIAKGQIVREIDAMGGQMGIVTDKTAIQFRMLNRSKGPAMWSPRSQSDRKHF
                     IEEWTKIVSNTDNLYIWQDTVKQFVFENNSVTGVITALGVEIRAKAVVLTAGTFLGGL
                     MHVGKQQMTGGRVSEPASFGITDQLKSIGFTTDRMKTGTPMRVDGRTIDFSHLTEQPG
                     DDDFHKFSYLKEIHRQLPQQSCWITYTNDKTHQVLNRGLEFSPLYNGQIQSIGPRYCP
                     SIETKIVTFADKDSHQLFLEPEGVHSTEWYINGFSSSLPLQTQIDAVHTVPGLENVQL
                     FRPGYAIEYDFFDPTQLKHTLETKKIKNLFFAGQVNGTTGYEEAAGQGLIAGINAHIN
                     VHGGNEFILHRNEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADERLTEKS
                     YELGLADNNRMEQYKNKKTGIDKLIDFLKITSVKPKDINAFLISNNSTELAHGCKLSD
                     LVLRPQLSINTLAEAVPSLRQQIENITEIREEIIEGAEIILKYSGYIERERLIADKLN
                     RLENIYIKDKLNYETIQSLSTEARQKLQKINPETIGQASRIPGISPSDVNILLVMMGR
                     "
     gene            complement(4078..5145)
                     /locus_tag="PLN75_00535"
     CDS             complement(4078..5145)
                     /locus_tag="PLN75_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HRG02632.1"
                     /translation="MRKLLLFLFILSLGSKAQNPVILTESMALKLAQMPIKSITLEFP
                     NKPGHIYNSAEEVNFSPRMLHPVFYGSFDWHSSVHGHWMLVRVLKLFPENKNFDEIVK
                     ILGNSFRIEKMRIEADYFSKFENAKTFERTYGWAWLLKLDNELMVWNHPNAHEWHNAL
                     QPLTNVIVDLWKAYLPKQTYPDRTGVHPNSAFALNFALEWARTKKDKEFENALIEKAL
                     NFYGKEQNTPAYLEPNGADFFSPSLQIASLMSKILPQKEFASWYNRFLSDKSFQNVCQ
                     IPVVSDLTDYQTVHLVGLSFSRAWCMKEIAKTLPIKNKNRNTLIITANKLADKALPLL
                     FAGNYGGDHWLASFALMALDN"
     gene            complement(5301..5528)
                     /locus_tag="PLN75_00540"
     CDS             complement(5301..5528)
                     /locus_tag="PLN75_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02633.1"
                     /translation="MDSSVLENVFGYIAMSLLVVSFIPKNMKVVRIINLIACMFFVGY
                     GIFLGWTWPIIISNMAVCCVQVYHLFIAKTN"
     gene            complement(5668..6024)
                     /locus_tag="PLN75_00545"
     CDS             complement(5668..6024)
                     /locus_tag="PLN75_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRG02634.1"
                     /translation="MEFTYDKNRPKSIFFEKAKVFPLEESIEYASGGVISKQITKSPA
                     GNVTLFSFDEGQGLTEHTASYDAMVQILDGEAEILIDETPHLVKKGECIIMPAGIPHA
                     LHAVRPFKMLLTMIKG"
     gene            complement(6088..7095)
                     /locus_tag="PLN75_00550"
     CDS             complement(6088..7095)
                     /locus_tag="PLN75_00550"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRG02635.1"
                     /translation="MKVAIVGASGAVGQEFLRVLEERNFPLTELVLFGSSRSAGKEYE
                     FKGKKLIVKELVHGDDFKGIDIAFTSAGASISKEFAEDITRFGAVMIDNSSAFRMEND
                     IPLVVPEVNAADAKNRPRGIIANPNCTTIQMVVALKAIEDLSHIKRVHVSSYQAASGA
                     GAAAMDELELQYRQISNGEQPTVSKFAYQLAYNVIPHIDVFTENGYTKEEMKMYHETR
                     KIMHSDVEVSATCVRVPTLRAHSESVWAETERPVSVEEAREAFSKAHGVILMDNPAEK
                     IYPMPLFLSGKDPVHVGRVRKDISNPNGITFWCVSDQIKKGAALNAVQIAEYLIAEKD
                     I"
     gene            7216..9015
                     /locus_tag="PLN75_00555"
     CDS             7216..9015
                     /locus_tag="PLN75_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRG02636.1"
                     /translation="MRKTVVFLCFLLPFFVFSQNKIEWKEDFSDGNFTENPLWSGTTE
                     NFAVNTGFQLQSKASAASKSFLTTYSEVFDNAVWEFWVKINYNPTSSNYASIYVISDR
                     ADLSGDVNGYYIQIGNTSDEVSLYLQQGSKRTKIIDGTDGRTNSNPAEINVKLIRDKD
                     GNFMLYSKLPSESDFYAEGSVQNLNVAGSKYFGLLFSNSSLTGNAYFFDNISVSGEKF
                     EDKLPPEWINLTLTEPNRMILNFSEPVDISQASFEADRGIGVPSEVIIAADKMKIELI
                     FNQNFEKGKIYTLNALNVKDLAGNLLVINQKKIGIVEKTDFGDLIINEILFEPATDIP
                     EYFEVFNHSSKILDLSKISFGTRKTDGSFTPANYFPDKTLILPGQYLTLTPDPEIIKN
                     TYSTPDTANIIFSSKWSALNNTGASFLITNQTGDSIYDEVKYDVKWHHSLIKNTKGVS
                     LEKLNPSLPSQSSESWHSASSQSGYGTPGYRNSQYREITAETSTEKWVWIEPEAFTPD
                     NDGTDDVCFIRYKTDAAGYTANVTVFSAVGVKMRQLASNVLLDADGFLSWDGRTDRGQ
                     NVNPGIYVLYFEMINTETGVKKTEKLPLVVSTR"
     gene            9095..9682
                     /locus_tag="PLN75_00560"
     CDS             9095..9682
                     /locus_tag="PLN75_00560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRG02637.1"
                     /translation="MDKISYALGLSLGNNLLSSGIKSLNIEKLARGIQDVLEQKKPEI
                     TYQEAQEIIDEYFRKLQEQSSEKTISEGKAFLESNGKRPEVITLKSGLQYEIINKGNG
                     NLPKASDKVRVHYHGTLIDGTVFDSSVRRGEPATFGVTQVISGWVEALQLMPVGSKWK
                     LYIPSNLAYGAQGAGQHIGPHTTLIFEVELLDIVK"
     gene            9787..10296
                     /locus_tag="PLN75_00565"
     CDS             9787..10296
                     /locus_tag="PLN75_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRG02638.1"
                     /translation="MKKQNFLTLVAFAAILGFVSCNSGYKTQEVSLKTQEDSLNYALG
                     VINGEGIRSSYFQNDSTNESLGQMMVALDKAFNSNNKDEMYKYGNQIGNMLKQQKSAG
                     LMGDSTLVFDQKLITLGLVNGLKGFTEGMTPDQAQQFIQTTMMKLQEAKNPVQPSVPA
                     DTTAAATVK"
     gene            10336..11214
                     /locus_tag="PLN75_00570"
     CDS             10336..11214
                     /locus_tag="PLN75_00570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRG02639.1"
                     /translation="MKKQNIFLVLAAVAVIGFTACNGGGVSTTAKAPKLTSQIDSLNY
                     AFGLANGNDLKMGMFQNSTDSVDKKIGAFIKGLNDGLSGPVEKNPQFSGVGNEFGSWL
                     NQQKESGLMGDSTLKLNYDIIKQAVINGIKKANTQMTPEQAQEYLNKTMMARQEKALL
                     EKHGAKKEAGDKFLAENGKKPGVVTTASGLQYEVVKAGTGAKPTASDMVKVHYKGTLL
                     DGKEFDSSYKRNEPATFGVGQVIKGWTEGLQLMPVGSKYKFYIPYNLAYGAQGTPDIP
                     PFSTLVFEVELLEIVK"
     gene            11254..12045
                     /locus_tag="PLN75_00575"
     CDS             11254..12045
                     /locus_tag="PLN75_00575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRG02640.1"
                     /translation="MKKYSLIAIAALFLTGCSSVKKVEIVPILPDVVMSNAVDSMSYA
                     LGINMGADFAKNIETIPGGKSNIDLIIKGFSQAMKKDSTLLKPEVAQEYFRKYIMAEQ
                     ARDNEAKKATGEKFLAENKVKQGIAITPSGLQYEVVQAAAGPKPQATDKVKVHYTGTT
                     IDGNVFDSSVQRGEPVEFALNQVIPGWSEGVQLMNVGSKYKFYIPYNLAYGEQGIPQA
                     NIPPFAPLIFEVELLDIVTPEPAPLVEEVKPAPVKPTAKSSTKKK"
     gene            12074..12580
                     /locus_tag="PLN75_00580"
     CDS             12074..12580
                     /locus_tag="PLN75_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02641.1"
                     /translation="MKKQNFLIMLSVIVALGLSSCGGSQQPKKANLKTQEDSLNYAMG
                     LVISEEILTNGLQNDSSEINAAFLVNKIEKFYKDKTSDKVYKMGMKTGDIIKEQRIKG
                     LYGNSTLTFNEELFKQGLLKGISADTTGLTGPHAIVYFQNTIQKIQNENRLKATSAPE
                     VPADSIQN"
     gene            12611..13483
                     /locus_tag="PLN75_00585"
     CDS             12611..13483
                     /locus_tag="PLN75_00585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRG02642.1"
                     /translation="MKRQHIIFTAGMLIMMTLSACNSSKTNSTKDPVLSSEIDSLNYS
                     YGVALGNDIKINLLSSDSIDQKTKSFIKGLEEGSKTEADEKPGNSALAAYFADLIKQQ
                     ESSGLFGDSTIRLNFKFIKQGLINGLNKSEIQMTPQQANEYINKVLNARQEVKMMSMF
                     GENKEAGESFLAENGGKAGVVTTPSGLQYEVIEAGNGPKPTDSDVVKVHYTGTLLDGS
                     EFDSSFKRNEPAEFSVNQVIPGWKEGLLLMPVGSKYRFYIPQQLAYGAKGARGVEPLS
                     MLIYDVELLEIVKK"
     gene            13681..14136
                     /locus_tag="PLN75_00590"
     CDS             13681..14136
                     /locus_tag="PLN75_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRG02643.1"
                     /translation="MEKIDKLDRKILRIISQNARKPFKDVAEECEVSRAAIHQRVQRL
                     IDMGVITGSGYSVNPKMLGFQLCVYIGITLERASMYKDVVAALEQIPEVVESHYTLGA
                     YTILIKMYAKNDEHLMELLNGKIQEIPGVATTETLTSLDQKIRRTVPIE"
     gene            14370..15365
                     /gene="trpS"
                     /locus_tag="PLN75_00595"
     CDS             14370..15365
                     /gene="trpS"
                     /locus_tag="PLN75_00595"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRG02644.1"
                     /translation="MKTVLSGIRPTGNLHLGNYFGALRNFVEMQHEYSCYFFIADLHS
                     LTTHPTPQNIQGNVKSVLAQYLAAGLDPEKATIYVQSDVPEVIELYLYLNMNAYVGEL
                     ERCTSFKDKIRQQPQNVNAGLLTYPTLMAADILIHKADFVPVGKDQEQHLEMARTFGN
                     RFNRMYGHEYFPEPQAFNFGRELVKIPALNGAGKMGKSEGDDSTIFLTDKPEVILKKV
                     KRAVTDAGPTQPNQPMAEPIANLFQLMNVVSQPETIQFFKEKYNDCSIRYGDLKKQLA
                     EDMITFVNPFRERIEDIMADEAFLNRVAEMGKEKAKASAGKTIREVREIIGFKPY"
     gene            15661..17181
                     /gene="purH"
                     /locus_tag="PLN75_00600"
     CDS             15661..17181
                     /gene="purH"
                     /locus_tag="PLN75_00600"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRG02645.1"
                     /translation="MVKKQIKRALISVYHKERLDLIIKKLHDEGVSFLSTGGTKTFIE
                     SQGIPCEAVEELTTYPSILGGRVKTLHPKVFGGILARRDVEKDAEQLTEYQIPEIDLV
                     IVDLYPFEATVAAGADEKNVIEKIDIGGISLIRAAAKNYKDVVILASQDQYMPFLELI
                     NQNGAETTLEERRWFAKEAFAVTSHYDSAIFNYFDGEDASAFRFTANGSKPLRYGENP
                     HQKGYFFGEFNEMFEQLHGKEISYNNLLDIDAAVNLINDFEDITFAILKHNNACGLAS
                     RDELKTAWKDALAGDPVSAYGGVLITNAIVDKDTAEEINKLFFEVIIAPDYDLDALEL
                     LTQKKNRIILVLENTKVPQKQFRSVLNGVLVQERDTYQETGEELKTVTEKEPDEDEID
                     DLLFANKIVKHSKSNAIVLAKDKQLIASGVGQTSRVDALKQAIEKAKSFDFDLKGAVM
                     ASDAFFPFNDCVEIAAEAGITAIIQPGGSVRDTDSIEMANQKGVSMVTTGIRHFKH"
     gene            17273..18292
                     /locus_tag="PLN75_00605"
     CDS             17273..18292
                     /locus_tag="PLN75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRG02646.1"
                     /translation="MGLFSFTQEIAMDLGTANTIIIQDDKIVVDEPSVVALDKRTDKL
                     IAIGEKARQMQGKENEGIRTVRPLRDGVIADFYACELMMRGMIKMIPQKSRLFSPTLK
                     MVVCIPSGSTEVEIRAVRDSSEHAGGRDIYMIYEPMAAAIGIGIDVEAPNGCMIVDIG
                     GGTTEIAVISLGGIVSNKSIRIAGDDLTADIMEYMGRMHNIKVGERTAEMIKINVGAA
                     LTDLEDAPEDYVVHGPNRMTALPMEVPVSYQEIAHCLEKSVSKIEAAVLSALEQTPPE
                     LYSDIVQSGVYLAGGGALLRGLDKRLTDKLNIKFHVAEDPLHAVARGTGIALKNVDKY
                     PFLMR"
     gene            18425..19297
                     /gene="mreC"
                     /locus_tag="PLN75_00610"
     CDS             18425..19297
                     /gene="mreC"
                     /locus_tag="PLN75_00610"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRG02647.1"
                     /translation="MKNLISFLIRYSVVFLFIILEIISLVLISRNKGYQRSVLLSSTN
                     TVVGKMYETSSKVVEFFKLSNANKNLAEENTFLLNRVTELENTLNTLTDTVNSNEWKT
                     VRIPPERQYDYIAAKVIRITTHQLQNYITINKGSLDSIKPDMGVVGDQGVVGIVEAVS
                     PHFAKVIPILHPKSTIVTKFKKSNYFGPLVWDGQDYRYAKLNDIARHVKFSLGDTLVS
                     SGFKSFPEGIIVGTINNFDIRESDAYYNIQVRLAVDFRSLTHVKVINYKNYQEQTELE
                     KGTPDEKTISKKDK"
     gene            19307..19804
                     /gene="mreD"
                     /locus_tag="PLN75_00615"
     CDS             19307..19804
                     /gene="mreD"
                     /locus_tag="PLN75_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRG02648.1"
                     /translation="MQLVIQNIFRFVMLVLVQVFVLDNIQFLGYVSPMIYVLFILSLP
                     VRFPKGVLLILAFILGLTVDIFNNTMGIHAFATVFAAFVRPVVINMSVSTEEMANPSP
                     SFRTFGVANYVKYVILIVLFHHIVLFLVESFSFMHLTLLVPKILLSSAVTILIILGIQ
                     SLKSK"
     gene            19840..21648
                     /gene="mrdA"
                     /locus_tag="PLN75_00620"
     CDS             19840..21648
                     /gene="mrdA"
                     /locus_tag="PLN75_00620"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRG02649.1"
                     /translation="MAVIMIVVVVIYILRLFSLQILEDKYKKGAASNAFLNKTIYPPR
                     GLLYDRNDSLLVYNKPAYDIAFINREIRNLDTLSFCNDLKITKEYFIERMKEVKNRDK
                     NPSYSSLTPQVFMTQLEIKDVAAVQQSMYKYSGFYIQTRTLREYRYAAGGHVLGSIGE
                     ITPRMLENDEEGYYDPGDYVGRDGFEFTYEKDLRGEKGVEVLLRDARGRIKGKYEEGR
                     KDIAPKAGKNIKMTLDIKLQILGEQLLAGKVGSVVAIEPNTGEILAMVSNPTFDPSLL
                     VGRERSKNYSLLVNDPTKPLMNRATQAQYSPGSSFKPFQALVALKQGAINEGTRFSCN
                     GPGSSPIRCTHHHGSPVSLLNAIEQSCNPYFWHTFESTLVKGGYGKDNQNFRKNYDEW
                     RINIMKFGFGARFEDSDIYQQSRGNIPSENYFDKVYKSKTGWRALTIRSLSIGQGEVL
                     VTPLQLANATSVIANRGFFITPHLNKSDSMLLHKHDVGVDKRHFQVVDEGMWRVNEFG
                     TARSYKVPGINWCGKTGTVQNNRGKDHAFYMGYAPRENPKIAIAVVVENAGFGSTFAA
                     PIASLMVEQYLTGKVVRTDLLTKTMNVRTNANVKEY"
     gene            21626..23092
                     /gene="rodA"
                     /locus_tag="PLN75_00625"
     CDS             21626..23092
                     /gene="rodA"
                     /locus_tag="PLN75_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRG02650.1"
                     /translation="MPTSRNISIKYALDWKTVFIYLFLVAFGWLNIYGASYDFDQASI
                     FDFSQRAGKQFVWILTAFGLAGVLLLLDSRLYSYFAYLIYIFVIFLLIVTIFIATDVK
                     GSRSWLVFGPVSFQPAELAKTATALALAKFMSAYNYTLKGWKDLIPLAVIVFIPFALI
                     ILQKETGSALVFLAFLLMFYREGMSGLVLLLGVFAVALFVIAIGFSEIPIQTDSGTLG
                     MVLAMLLVILVQFVFAFFYKRNRKEALLLLAGVAGMSIIAVVLNIWLPVNYDYVAIFI
                     VLLSCVYWFIISRFKRMKQYLFIAWISLGAMLFCFSADYLFYQVLEPHQQIRIKVLLN
                     MEEDLRGAGYNVNQSKIAIGSGGMFGKGYLNGTQTKLKYVPEQDTDFIFCTVGEEWGF
                     LGSSVLLFIYLWLLLRIIRIAERQNEVFNRVYGYSVASIFFFHLTINVGMVLGLVPVI
                     GIPLPFLSYGGSSLWSFTVLLFILLRLDAARLQRGVQR"
     gene            complement(23119..23616)
                     /locus_tag="PLN75_00630"
     CDS             complement(23119..23616)
                     /locus_tag="PLN75_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02651.1"
                     /translation="MESKQPIVNEETECCPKFEPERWDEKTLEWKNKKFVKGSVCTFM
                     YMPLNFGAVMRKLDTKIRNSGASMQQDGMGLSDHTSAWKMDVCLAVDREVAGLNNVTF
                     SGKFLCKVYEGDFRETGKWTKNYRDYAKGKNLEIEKMYMWYTTCPKCAKKYGKNYVAI
                     LGKIG"
     gene            23786..24127
                     /locus_tag="PLN75_00635"
     CDS             23786..24127
                     /locus_tag="PLN75_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRG02652.1"
                     /translation="MNGKLIACCGINCATCDACIATMTHDEELRAKTAEKWSVEYNSS
                     DITTETLYCTGCRQAGAKFAYCEFCEIRNCVKSKGFETCGECDQLENCSIVGNVLKIL
                     PEALDNLKSLN"
     gene            complement(24315..24626)
                     /locus_tag="PLN75_00640"
     CDS             complement(24315..24626)
                     /locus_tag="PLN75_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02653.1"
                     /translation="MLRVYELIRASGYTLKEIAYKLGITEEELAGNIRENSLTETLEN
                     IARILEVNVGDFFDKNCDDEEICGIVIFRKKTYKITSRQNLEKLYEDVHRGFYQVKSY
                     F"
     gene            complement(25118..26434)
                     /locus_tag="PLN75_00645"
     CDS             complement(25118..26434)
                     /locus_tag="PLN75_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRG02654.1"
                     /translation="MNWQQLLSPMRLGMEDFHDRERDNRNQFQRDYDRLIFSAPFRRL
                     QNKTQVFPLPGSIFVHNRLTHSLEVASVGRSLGVNIAQAHRNEQVNYFDEIGAIVSAA
                     CLAHDLGNPPFGHSGEEAIATYFSEGEGIFLKDKLTETEWADFTRFDGNANAFRLLTH
                     QFNGRRPGGFVLTYSTLASIVKYPYASVHSDKKKQKFGFFDSERSDYEKIAVELGISR
                     QKNNPDRFARHPLVYLVEAADDICYQVMDIEDAHKLKIVTTDETKEMYFRFFDEKRLK
                     RIRETLSLVSDVNEQIAYLRSSVIGQLVNECSSAFLNNEEAILNGDYEGSLIKHVSVH
                     TSEAYKNCSEVAVNKIYKSQEVLDIELAGYRIIYQLMEFFVNAVLHPEKTYSKLLLDR
                     VPAQYDIKNPTTYGKVQAVLDYISGMTDVYALDLYKKITGINISSI"
     gene            complement(26431..26724)
                     /locus_tag="PLN75_00650"
     CDS             complement(26431..26724)
                     /locus_tag="PLN75_00650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG02655.1"
                     /translation="MDIQVKTNHNDKHGEFMLLVNGVASGKMTFVFTEKNVISVNHTG
                     IDESFKGLGYGVYLMEEMVKFSRENELKVIPVCPYVVKMMDRTPDYQDVLYVK"
     gene            complement(26786..27676)
                     /locus_tag="PLN75_00655"
     CDS             complement(26786..27676)
                     /locus_tag="PLN75_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRG02656.1"
                     /translation="MLKKLKSYMMPIAMLTGAALYPLFSRLAFLTPYLIFVMLFLTYN
                     KLNLSHIRLKRMHFWLIFFQIFVSTTIYFLLRPVNEILAQGIMICVLVPTATSAPVIT
                     HMLKGNVENLTAYMLLSNLMVVILAPVLFSYANNTLEIGFFSSFLTISEHIVLLLLGP
                     LVLAMITKRLLPKVGSRLGSVSGLSFYIWTFALIIVTARTVNFIIMQGKESYATEILL
                     AAGTLAVCLIQFFTGKLIGKAYGETIAGGQSLGQKNTVLAIWMAQTYLLPLSSVGPGA
                     YVIWQNIFNSLQIWRKRKSL"
     gene            complement(27727..28848)
                     /locus_tag="PLN75_00660"
     CDS             complement(27727..28848)
                     /locus_tag="PLN75_00660"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRG02657.1"
                     /translation="MIKCIKYILVSSLVSLSLISCENEKPSGNVTTSREFIDIDEVFP
                     IYNGNMANGWTGDIMVAMDGSLLADRISVDGGVTWSARIPLLGGAGRVLDETTGDILK
                     LDPGGAGRKPENFMYRSKDSGFTWVKEEIVRHPDINGWLPIISGCEEGITLRHGKHKG
                     RLLAPARVLIDYNASNDSLLPYHYSSAIYSDDGGKTWFTSKPYPVAGTGEAALVELSD
                     GTVYYNSRNHNRPGNRDIAFSYDGGETWVDQYIDPVLPDGPPNVYGNKAGLVRLEIEG
                     YDILVFSMTDNPYGPTAIHSTAGRINITVWASFDGGKTWPVKRLVSPHGGYSHLAAGR
                     PKTPSDGLIFLTTDGIFARFNLDWITYGRDWHLFLPKKP"
     gene            complement(28845..29540)
                     /locus_tag="PLN75_00665"
     CDS             complement(28845..29540)
                     /locus_tag="PLN75_00665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRG02658.1"
                     /translation="MIQDKDLGKIEFKQNIRARRMSIRILSDSLQVNLPVGYSENDGL
                     KFIEKIRLKLIKRQKSISSQTIKLNEGKTLTTQTFDIHIRKAERNTLYASLKSGILNI
                     EFPQHLSVNDVQTQTYFWNAVNYFLRKEAKRILPHRTQMLAGKYGFSYTDVKIQSSKT
                     RWGSCNRKKNINLSFYLMLLPEYLADYVILHELCHTKEMNHSPRFWEWMDRVTDGKAK
                     KYRTELKNYSIPK"
     gene            29655..30308
                     /locus_tag="PLN75_00670"
     CDS             29655..30308
                     /locus_tag="PLN75_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG02659.1"
                     /translation="MIHATNIHKSFGELEVLKGVDLVVNKGEIVSIVGPSGAGKTTLL
                     QILGTLDKPDSGNVMIDGLDVSKLNSDRISEFRNKHVGFIFQFHQLLPEFTALENVTI
                     PALIGRTDFRKATQRAKELLDFLQLSDRLEHKPAELSGGEKQRVAVARALINNPSLIL
                     ADEPSGSLDTQNKEELHKLLFQLRDKFGLTEVIVTHDHDLAKICDRTIEMKDGRIGG"
BASE COUNT         9030 a         6128 c         6239 g         8915 t
ORIGIN      
        1 tttttagata atctaaaaaa gattatttta taaatcttac aaattccaaa aatcttacat
       61 tttatcctaa tttttcgtag atatttcttc ccacctgtcc gttcaaataa atatattgaa
      121 ttctcaagcc tttaaatttg ggttaaaatg actgtaaacc tttgaagctc tgtgagttcc
      181 gatgatattt tcaatgtcag aaaattcagc tgttttgatg cgttttaagc ttttaaaatg
      241 agcaatcagt tcattttttg ttttttctcc aattccggga attttatcaa gctcagaagc
      301 tgtttgagtt ttgcttcttt tttcacgatg aaatttaata gcaaaccggt gaacctcttc
      361 ctgaatattt gccattaata tgaaaagtgt ttcattcggt ttcattccta tttcttttgg
      421 aggaaagcca aaaagtagtt cccgggtttt atgctttgta tttttagcca gacccacaat
      481 cggaattttc aggtttaact catcttctac cactttacgg attgcttcca tttgaccgac
      541 accgccatca gccactatca aatcgggaaa ttcgctctct tcttcgatca tccggctgta
      601 tcttcttctc accacttctt tcattgatgc gtagtcatcg ggtccttcca ccgtttttat
      661 cgtgtatttc cggtaatctt tcttggatgg ttttcctttt ttgaacacca cgcatgcagc
      721 tactgcatcc gaacctgaaa tgtttgaatt gtcaaatgct tcgatggtca ttggaagttt
      781 atccagttga agtttttcct gcatttctgt cagaagtcgt attgcttttt gatccggatt
      841 cagtttttct gcttgtttta gtttttcaag cttatactgc tttacatttt gtagggataa
      901 atcgagcaat tttttacggt caccacgttc aggaaagata aattcaacgc cggaaatgtt
      961 ataatccacc gggaagggca ggatgatttc tttggaagtg ctctctaatc gttcccgcag
     1021 ttctacgata gcggtggcta aaatatcctc tttcgtctca tccagttgct tccggtattc
     1081 tatggtgtat ccttgaataa tcgaaccttt tgaaatccgc agaatgttca cataagccga
     1141 actctcatct tcctcgtaac caaaaacgtc ggtattattt actaccgagt ttgctacgat
     1201 tgattttgct ttataatttt caatcaaatc gtatttctgt tttatctcat ttgcctcttc
     1261 aaaacgaaat tttgcagcca gttttttcat tttttccaag agcattttgc ttatttcgtt
     1321 ggcattacct ttgatgattt gacgaatttc atcaatatcc tggttgtaat cttctttgct
     1381 ttgcaatcct tcgcaaggac ctttgcattt ttttatgtga tattccaggc aaagtttgaa
     1441 ttttccttcc gaaattttag tctcggccag gggtagggaa caggttctga ttttataaag
     1501 ctgatgaata atatctaaaa ttgcattgat tgtccagact gaactaaacg ggccgtagaa
     1561 ttcacctatt cttttatcaa tgtttcttgt cttgaaaata cgtggaaatt cttctttggt
     1621 aatgcaaatg cttggatagg ttttaccgtc tttcatcatt gcattgtaac ggggcatata
     1681 ctctttaatc aaattatttt ccagtaaaag cgcatcttcc ggagtatcga ctaaaataaa
     1741 tctaaggtaa gatatatttt ttacaagtac gtttagtttt gcaatatcat gttttttatt
     1801 gaaataagat gaaaccctgc gcttcaaatt tttagctttt cccacataaa ttatctcatc
     1861 tttctcatta aaatattgat atactcccgg ggtttctgga agtaaatcaa tctgattttt
     1921 taatgattgt ttttttgtca tttcttagtt cgataatttt tatagtgagg tatgtagcct
     1981 aatttcattt gttccacggg gaacaaattt tcctgattat cctattgttg atcttttttt
     2041 agattcaaag tttttcatag aacatataac ataatccgca tatagtcaat tctttatttt
     2101 tgttttgttc cacgtggaac aaaacaactg tttttttatt ggcagcactc agcgaagtca
     2161 ccccctgggg agatttagag gtgctgttat cttcccatca ttaccaaaag aatattcaca
     2221 tcgctcggcg aaattccggg aattcggctg gcttgcccga tagtttccgg attgattttt
     2281 tgcaatttct gacgggcttc tgtggataaa gattgtatag tttcgtagtt caatttgtct
     2341 ttgatgtaga tattttccaa tctgtttagt ttatcagcta ttaatctttc gcgctcgatg
     2401 tagccggagt atttcaaaat aatttctgct ccttctatta tttcttctct tatttcagta
     2461 atgttttcaa tttgctgccg gagcgaagga actgcttcag ccagcgtgtt tatgctgagt
     2521 tgcggtcgca aaaccaggtc agacaattta catccatgtg ctaattcagt cgaattatta
     2581 gaaatcaaaa atgcattgat atcctttggt tttacggaag tgattttcag gaaatcaatc
     2641 agtttatcga ttccggtctt tttgttctta tattgttcca tgcgattatt gtcagccaat
     2701 cccagctcat acgacttttc ggtcaatcgt tcatcggcat cgtcctgacg gagaagaatg
     2761 cggtattcgg cacgcgaggt aaacatccgg tagggctcat ccacgccttt agttaccaga
     2821 tcgtcaatca atacgccgat gtaagcctcg tttctgtgta gaataaattc atttccacca
     2881 tgaacgttga tgtgagcatt gattccggct atcagaccct gacccgcagc ttcttcatat
     2941 cctgtggtac cgtttacctg accggcaaaa aacagatttt ttattttttt tgtctcgagc
     3001 gtatgtttta actgtgtcgg gtcaaaaaag tcgtattcaa tcgcgtagcc cgggcggaaa
     3061 agttgtacgt tttccaaacc aggaacagta tgaaccgcat cgatttgagt ttggagtggg
     3121 agtgaggaag aaaaaccatt gatgtaccac tcggtggaat gcacgccttc gggttccagg
     3181 aataactgat gtgaatcttt atcggcaaat gttactattt ttgtttcgat actcggacag
     3241 taacgcggac cgatactttg aatctgaccg ttgtaaagag gcgagaattc aagcccccga
     3301 tttaatacct gatgagtttt atcatttgtg taggtaatcc agcagctttg ttgaggaagt
     3361 tggcggtgaa tttctttcag gtaagagaat ttatgaaaat catcatcacc cggttgttcg
     3421 gttaagtgtg aaaaatcaat cgtgcgccca tcaacccgca tcggagtgcc cgttttcatc
     3481 cggtcggtgg tgaatccgat tgatttcagc tgatcagtaa ttccgaaaga ggcaggttct
     3541 gaaactcttc cacctgtcat ttgctgtttc ccgacgtgca tcagtccgcc aaggaatgtt
     3601 ccggcagtta gtaccacagc tttagccctg atttcaactc ccaacgctgt aattacaccg
     3661 gtaactgaat tattttcaaa aacaaactgt tttaccgtat cctgccagat gtataagtta
     3721 tccgtattcg aaacaatttt tgtccattct tcaatgaaat gtttacggtc actttgcgaa
     3781 cgggggctcc acatagccgg tcctttcgag cggttaagca tccggaactg aatggctgtt
     3841 ttatccgtta caattcccat ttgtccgccc attgcgtcaa tttcgcgcac aatctgacct
     3901 ttggcaatac cacccacggc aggattgcag ctcatttgag ctatcttgtt catatccatc
     3961 gtaataagca gggtttttga acctaaatta gcggcagcag cagcagcttc acagcctgca
     4021 tgaccggcac ctatcacaat gatatcgtat ttgaaatcca tttattattg ttttttttta
     4081 attatctaat gccatcaatg caaaactggc caaccaatga tctccaccgt aatttccggc
     4141 aaaaagaagt ggtaaagctt tgtcagctag tttattagct gtgattatta atgtatttcg
     4201 gtttttattt ttaatcggta aagttttcgc aatttccttc atgcaccagg ctcttgaaaa
     4261 tgataatcca accaggtgaa ccgtttgata gtcagttaaa tcgcttacaa ccggtatctg
     4321 gcaaacattt tgaaaacttt tatcggataa aaaccggttg taccagctgg caaattcttt
     4381 ctgtggaaga attttactca tcaacgaagc aatttgcaac gatggcgaaa agaaatcggc
     4441 gccgtttggt tctaaataag caggtgtatt ttgttctttt ccgtaaaaat tcaatgcctt
     4501 ttcaatcaac gcattttcaa actccttatc cttttttgtt ctggcccatt ccaatgcaaa
     4561 attcagtgca aaagccgaat tcggatgtac acccgttctg tccggatagg tttgcttggg
     4621 cagatacgcc ttccacagat ccacaatgac attcgtaagc ggttgcaaag cgttgtgcca
     4681 ctcgtgcgcg ttgggatgat tccataccat cagctcgttg tccaacttca acaaccaggc
     4741 ccagccataa gttctttcaa aagtttttgc attttcgaat tttgaaaaat aatcggcttc
     4801 aatcctcatt ttctctatcc ggaatgaatt accgagaatt tttacgattt catcgaagtt
     4861 tttgttttcg gggaataact tcaacaccct cacaagcatc cagtgcccgt gtacgctgct
     4921 gtgccagtca aacgatccgt aaaacaccgg atgaagcata cggggtgaaa agttcacttc
     4981 ttctgcgctg ttgtagatat gtccgggttt gttcggaaac tcaagggtaa tgctttttat
     5041 tggcatctga gccagtttca aagccatact ttcagtcaga ataacgggat tttgtgcttt
     5101 tgaacccaac gataggatga ataaaaaaag aagtaatttt ctcatttttt aatagtgaaa
     5161 ttaaatctca gtgtgcaaag ttacgaagtt ttatactttt gtcaataaac cacttaaagc
     5221 attaacgctc atttaaaatg acaaaacgac taagaacagc ggaatgtttt tagtcgttga
     5281 gcaaattatt aatatttcaa tcagtttgtt tttgcaataa ataaatgata aacctgaaca
     5341 cagcaaactg ccatgttaga aataataatc ggccaggtcc agcccagaaa gataccatat
     5401 ccgacaaaaa acatacaagc aattaaatta ataatccgaa cgactttcat gtttttgggg
     5461 ataaaagata cgacaagtaa actcattgca atgtatccaa aaacattttc taaaacagag
     5521 ctatccatgc tgaatttttt aaaattagtc ggcaaagata cggatatttt ggttaaatgc
     5581 aattcttaat ctcttatgct tgatttcaat acctgattct gatgttgtgt tttcggttct
     5641 ccaaccgaaa gttatcagat tgtaagttta tccttttatc attgtcagta gcattttgaa
     5701 tggccgtaca gcatgaagtg catgcggaat tcctgcaggc ataatgatac attcgccttt
     5761 tttcaccaga tgaggtgttt cgtctatcag aatttcggct tcgccatcca gaatttgtac
     5821 catggcatcg taggaggccg tgtgctcggt aagaccctga ccttcgtcga atgaaaacag
     5881 ggtgacattt ccagcaggtg atttggttat ctgcttgctt attacacctc ccgaggcata
     5941 ttcaatggac tcttcgagag gaaatacctt tgctttttca aaaaagatac ttttcggtct
     6001 gtttttatcg tatgtaaatt ccatattgca aaaaataaga taaatacttt gtaactgaaa
     6061 atgaaagtat tatgttaagc gagcccgtta aatatccttt tcggctatca ggtattcagc
     6121 aatctgaaca gcattcagcg cggctccttt cttgatttga tcgcttacac accagaaggt
     6181 gatgccattt ggattggaaa tgtccttacg cactctgcca acgtgaacgg gatctttacc
     6241 ggaaaggaaa agcggcatcg gatatatttt ttcagccgga ttgtccatca gaatgacacc
     6301 atgagccttt gagaatgctt cgcgggcttc ttcaacgcta accgggcgtt cggtttcggc
     6361 ccaaacactt tctgaatgag ctctcaatgt aggtacacgt acgcaggtgg cacttacttc
     6421 cacatccgaa tgcatgattt ttcgggtttc atgatacatt ttcatctcct ctttggtgta
     6481 tccgttttca gtaaatacat caatatgagg aattacatta taagccagct gataagcaaa
     6541 tttactgacc gtaggttgtt caccgttgga aatctgacgg tattgcagtt ccagctcatc
     6601 catagccgca gctcctgctc cgctggcagc ttgataggat gacacatgaa cccgtttgat
     6661 atgcgataaa tcttcgatag ctttcagtgc aacaaccatc tgaatagtag tacaattcgg
     6721 attggcaatt attccgcgtg gacggttttt agcatcggca gcattcactt cgggtactac
     6781 cagcggtata tcattttcca tacggaatgc acttgaatta tcaatcatta ccgctccgaa
     6841 tcgggtaata tcttcagcaa attcttttga aatacttgct ccggctgatg taaaagcaat
     6901 gtcaatgccc ttgaaatcgt caccgtgaac cagttctttc acaatcagtt ttttaccttt
     6961 aaattcatat tccttacccg cactgcgtga agaaccaaat aaaaccagtt cggtgagtgg
     7021 aaaattccgt tcttccaata cccttaaaaa ttcttgtcct acggcgccgc tggcgccaac
     7081 gattgcaact ttcataattt ttttatcgtt taatgtaact gataatttga aataataact
     7141 atttttgttt gtaaaatgtt ttctcaacat tattttttgc aaaagtaatt caattttaaa
     7201 ccaaatgctt gatttatgag aaaaacagtc gtttttttat gctttttgtt gccttttttt
     7261 gttttttcac aaaataaaat cgaatggaaa gaagattttt cagacggaaa ttttactgaa
     7321 aatccgcttt ggtccggaac tacagaaaac tttgctgtaa ataccggctt tcagcttcaa
     7381 tcgaaagctt ctgcagcttc aaagtcattt ctgaccacat attctgaagt attcgataat
     7441 gccgtgtggg agttttgggt gaaaatcaac tataatccta cttccagcaa ttatgcttcg
     7501 atatatgtca tttcagacag agcagatctt tcaggtgatg taaacggata ttatattcag
     7561 ataggaaata cttccgatga agtttcgttg tacctgcagc aaggttcaaa gaggactaaa
     7621 ataatcgacg gaaccgatgg gcgaaccaac tcaaatcccg cagaaattaa tgtaaaactg
     7681 attcgggata aggatggtaa ttttatgtta tacagcaagt tgccttcaga atctgatttt
     7741 tatgccgaag gcagcgtaca gaacctgaat gtggcaggaa gcaaatattt cggtctgctt
     7801 ttctcaaatt catctcttac cggaaatgcg tatttttttg acaacatatc agtttcgggt
     7861 gaaaaatttg aggataagct gccgcccgaa tggataaacc ttacactgac agagccaaac
     7921 agaatgatac tgaatttttc agaaccggtg gatatttcgc aagccagttt tgaagctgat
     7981 agagggatag gtgtaccatc tgaagtaata atagctgcag ataaaatgaa gattgaactg
     8041 attttcaacc aaaattttga aaaaggaaag atttatacgc tgaacgcact caatgtaaag
     8101 gatttagcag gtaatctgct ggttatcaat caaaaaaaga ttggaatagt tgaaaaaaca
     8161 gatttcggtg atttgattat caacgaaatt ctttttgaac cggctaccga catacctgag
     8221 tattttgaag tatttaatca ttcatctaaa atacttgacc tgagtaaaat tagttttggg
     8281 acgcgtaaaa ccgatggttc gtttactccg gccaattatt ttccggataa aaccctgatt
     8341 ctgcccggcc agtatctgac actgactcct gatccggaaa ttattaaaaa tacttattcc
     8401 acgcccgata cggctaatat cattttttca tccaaatgga gtgctctgaa caacaccggt
     8461 gcaagttttt tgataaccaa ccaaacaggc gattccattt acgatgaagt gaaatacgat
     8521 gtaaaatggc atcattcgct cattaaaaac acgaaaggcg tttcgcttga gaagttaaat
     8581 ccgtcgttac catcgcaaag ttcagaaagc tggcattcgg catcttcaca atcaggttac
     8641 ggaacacccg gctatcgaaa ctctcagtac agggaaatca cagctgaaac gagtaccgaa
     8701 aaatgggtat ggattgagcc ggaagctttt acacccgata atgatggaac agacgacgta
     8761 tgttttatcc gatacaagac agatgcagca ggatatacag ccaatgttac cgttttcagt
     8821 gcggtaggtg taaagatgag gcaattggcc tctaatgtac ttcttgatgc tgacggattt
     8881 ctctcctggg acggcaggac tgaccgcggt caaaacgtaa atccgggtat atatgtgctt
     8941 tactttgaga tgattaacac cgaaacaggt gttaaaaaaa cagaaaaatt acctctggtg
     9001 gtttcaaccc gatgaatttt agttatttaa caacttatat ctcaaataaa tcaactatct
     9061 ttgcagtcat ttttttaaca aataactact taagatggat aaaataagtt atgctttagg
     9121 tttaagtttg ggaaacaact tattgagcag tggaattaaa tcattgaata tagaaaaact
     9181 cgcccgcggc attcaggatg ttttggagca aaaaaagccc gaaataacct atcaggaagc
     9241 ccaggaaata attgacgaat atttccggaa actacaggaa caatcaagtg aaaagactat
     9301 ttcagaagga aaagcatttt tggaatctaa cggaaagcgt ccggaagtga ttacgctcaa
     9361 aagtggtttg caatatgaaa taataaataa aggaaacggt aatctgccca aagcaagcga
     9421 taaagtaaga gtacattacc acggaacttt gattgacgga acagtattcg acagctcggt
     9481 gcgacggggc gaaccggcta cttttggtgt aacacaggtc atttcgggat gggttgaagc
     9541 cctgcaattg atgcccgtag gctccaaatg gaaattgtat attccttcaa atcttgcata
     9601 cggtgctcag ggagcaggtc agcatatcgg accgcacacc acccttattt ttgaggtaga
     9661 attactggat attgtaaaat aaataaatac aaatcaggtt tttattaaaa acaaactgaa
     9721 ataaatcccc tgataaaatt aacttctgga aaaaggaata aaaataatag attaataaac
     9781 aaaaaaatga aaaaacaaaa ttttcttact ttggtggctt ttgctgctat tttaggtttc
     9841 gtatcttgta attcaggtta caagactcag gaagtgtctc ttaaaacaca ggaagacagt
     9901 ttgaattatg cccttggtgt aatcaacgga gaaggtatca gatcatctta tttccaaaac
     9961 gactctacca acgaaagtct gggacaaatg atggttgctt tggataaagc attcaacagc
    10021 aacaacaaag atgaaatgta caaatacgga aatcaaatcg gcaacatgct gaaacagcag
    10081 aaatcagccg gcttgatggg cgattccact ctggtttttg atcagaaatt aatcaccctg
    10141 ggactcgtta acggactgaa aggatttacc gaaggaatga cgcccgatca ggctcagcaa
    10201 tttatccaaa caactatgat gaaactgcag gaagctaaaa atccggtaca accaagcgtt
    10261 cccgctgaca ctactgctgc agctactgta aagtaataaa tttaaatcgt caaaacatta
    10321 aacagagaaa ttaaaatgaa aaagcaaaat atatttttag tattggcagc tgtagcagtc
    10381 atcggattca cagcatgtaa tggcggaggc gtatccacca cagcaaaagc acctaaactt
    10441 acttctcaga tcgacagttt gaactatgca ttcggtctgg caaacggaaa tgacctcaaa
    10501 atgggaatgt ttcaaaactc aacagattca gttgataaaa aaattggtgc atttatcaaa
    10561 ggattgaacg atggcctgag cggacctgta gaaaaaaatc cgcaattcag tggagtagga
    10621 aacgaattcg gaagctggct aaaccaacag aaggaatccg gactcatggg agactcaaca
    10681 ctcaaactta actatgacat tattaagcag gcagtaatca acggcattaa aaaagcaaat
    10741 acacaaatga cacctgaaca ggcacaggaa tatctcaata aaacgatgat ggcccgtcag
    10801 gaaaaagcat tgctcgaaaa gcacggtgct aaaaaagaag ccggagataa atttctggcc
    10861 gaaaacggca aaaaacccgg tgttgttact accgcaagtg gattgcagta cgaagtggtg
    10921 aaagcaggaa caggcgccaa accaactgct tctgacatgg taaaggttca ctacaaagga
    10981 acactgctgg atggaaaaga attcgacagt tcgtacaaac gtaacgaacc ggcaacattt
    11041 ggtgtaggtc aggtaatcaa aggatggaca gaaggacttc agttgatgcc tgtaggatca
    11101 aaatataaat tctacatccc ctacaatctg gcatacggtg cgcagggcac tcccgatata
    11161 cctcctttct ccaccctggt attcgaagta gaattacttg aaattgtaaa ataaacgaat
    11221 aagctacaaa tagtcattat tttaactatt ccaatgaaaa aatattcatt aatagctata
    11281 gcagccttgt ttttaacagg ctgctcttct gttaaaaaag tagagatagt acctatttta
    11341 ccggatgtcg taatgtcaaa tgctgtagac tccatgagct atgccttagg aatcaatatg
    11401 ggagcagact ttgctaaaaa tatcgaaacc attccaggag gaaaatccaa tattgatctt
    11461 attataaaag gattttcaca agcaatgaaa aaggattcga cgctgctaaa acccgaagta
    11521 gctcaggaat atttccgaaa atacatcatg gccgaacaag ccagggacaa cgaagccaaa
    11581 aaagctacag gagaaaaatt tctggccgaa aataaagtaa aacaaggtat tgccatcact
    11641 ccgagcggac ttcagtatga agtggttcag gcagctgccg gaccgaaacc acaggcaacc
    11701 gacaaggtaa aggtgcatta caccggaact acaatagatg gaaacgtctt cgatagttcg
    11761 gtgcaacgtg gcgagccggt agaatttgca cttaatcagg ttatacccgg ttggtcagaa
    11821 ggtgtacagc ttatgaatgt ggggtcgaaa tataaatttt acatacccta caacctggct
    11881 tatggtgagc agggtatccc gcaagccaat attcctcctt ttgcaccatt gattttcgaa
    11941 gtagaattgc tggacattgt gactcccgaa cccgctccgt tagtagaaga agtgaaaccc
    12001 gcaccagtga aacccaccgc aaaatcatca acgaaaaaaa aataaaagaa acagattaat
    12061 aattatttaa aatatgaaaa aacagaattt tctgataatg ctatcagtaa ttgttgcttt
    12121 aggactatct tcgtgtggag gcagccaaca acccaaaaaa gctaatctga aaactcagga
    12181 agatagcctg aattatgcaa tgggactcgt gataagtgaa gaaatactta ccaacgggct
    12241 tcaaaacgat tcatccgaaa taaacgcagc ttttttagta aataaaatag aaaaatttta
    12301 taaagataaa accagcgaca aggtatataa aatgggaatg aaaacagggg atataatcaa
    12361 agagcaaaga atcaaaggac tttacggtaa ttctaccctg actttcaatg aagaactttt
    12421 taaacaaggc ctgttaaagg gaatttcggc tgatactacc ggtcttaccg gtcctcacgc
    12481 aatcgtttac tttcagaaca ccattcaaaa aatacagaac gaaaatagac tgaaagcaac
    12541 ttcagcgcca gaagtacctg ccgattccat tcaaaattaa tcattcattc atagaaaaaa
    12601 cagtaaaaaa atgaaaaggc aacatataat tttcactgca ggcatgttaa taatgatgac
    12661 tctctcagct tgcaacagta gtaaaacaaa ttcaaccaaa gacccggtgc tttcatccga
    12721 aattgacagt ttgaattact cctatggcgt ggcattaggc aacgatataa aaattaacct
    12781 cctgagctct gactccattg atcaaaagac taaatcattt atcaaaggac tggaagaagg
    12841 atctaaaacc gaagcagatg aaaaacccgg caattcagct ttagctgcgt attttgcaga
    12901 tttgatcaaa caacaggaaa gttcaggatt atttggagac tctacaatac gtctgaattt
    12961 taaatttatc aaacagggat tgataaacgg tctgaataaa tctgaaattc agatgactcc
    13021 tcagcaagcc aacgaataca tcaacaaagt gttgaatgcc cgtcaggaag ttaaaatgat
    13081 gagcatgttt ggcgaaaaca aagaagccgg cgaaagtttt ctggccgaaa acgggggaaa
    13141 agcaggcgtt gtaactacac cgagcggact tcaatacgaa gtgatagaag ccggaaatgg
    13201 tccgaaacca accgatagtg atgttgtaaa agttcactat acaggcacat tgcttgatgg
    13261 ctcggagttc gacagttctt ttaaaagaaa tgaaccggct gaattttctg ttaatcaggt
    13321 tattcccgga tggaaagaag ggctactgct tatgccggta gggtcaaaat atagatttta
    13381 tattcctcag caactggcat acggagcaaa aggagcaaga ggagtagaac ctctcagcat
    13441 gttgatttat gacgtagaac tgcttgaaat cgtaaagaag taaaaaaata aagctaattt
    13501 tacgatacgg gaatatcatg ggatattccc gtatttcgtt tttacgaaca atcatctttt
    13561 tcgtttttag gaactttttt ttgaaaaaag taaacgaaaa taaaaatatt ttacaaaaaa
    13621 gttttgttct ttttcaaaaa tatgtatttt tgttgtcata atgataattt ttgcattacc
    13681 atggaaaaaa tagataaatt agaccggaaa attttacgta taatttcaca aaatgcacgt
    13741 aagcccttca aggatgtggc tgaagaatgt gaagtatcca gagctgccat ccatcagcgt
    13801 gtccaacgcc tcattgatat gggtgtaatc accggctcgg gatattcggt aaatccgaaa
    13861 atgctcggat ttcagctgtg tgtctatatc ggcatcacgc tcgaaagagc ttcaatgtac
    13921 aaagacgtgg tagcagcact cgaacaaatc cctgaagtgg tagagtcaca ttacaccctg
    13981 ggtgcataca ctattctgat taaaatgtat gccaaaaacg acgaacacct gatggaattg
    14041 ctcaacggaa aaattcagga aatacccgga gtagctacta cagaaacgct tacatcgctc
    14101 gaccagaaga tcagaagaac agttccgatt gaataagctt actcaggaat tcattttttt
    14161 tggagacgat aaaaaacttc ataaaccgct taactgttta agcggtttat ttgctttaaa
    14221 atgattaact ttgcaccctt aattcaggta atcagtcagt ttgcagaccg gtagatcaaa
    14281 ctttgtgaag taatcagccc gccaacatac aacagaattc aattgaactt attaccaaat
    14341 acactattca acttttttga aaaccaatga tgaagacagt tttaagcgga attcgcccta
    14401 caggaaattt acatctcgga aattactttg gagcgcttcg taactttgtg gaaatgcagc
    14461 atgaatacag ctgctatttc tttatagccg atttgcactc actcaccact caccctacgc
    14521 cccaaaatat tcagggaaac gtaaaaagtg tactcgccca gtatctggca gccggacttg
    14581 atcccgaaaa ggcaaccatt tacgttcaaa gcgatgtacc tgaagtgata gaactttacc
    14641 tctatctcaa tatgaatgcc tatgtgggag aactcgaacg ttgcacctca ttcaaggaca
    14701 aaatacgcca gcaaccgcaa aatgtgaatg ccggattgct cacgtatcct accctgatgg
    14761 cagccgatat tctcatacac aaagccgatt ttgtgcccgt aggaaaagac caggagcaac
    14821 acctcgaaat ggcacgtacc tttggcaacc gatttaaccg aatgtatggg cacgaatact
    14881 tccccgaacc ccaggctttc aatttcggac gcgaactggt aaaaattccg gcacttaacg
    14941 gagccggaaa aatgggaaaa tcggaaggcg atgacagcac aatattcctg accgataagc
    15001 ctgaagtgat cctgaaaaag gtaaaacgag ccgttaccga tgcaggacct acccaaccca
    15061 accaaccgat ggcagaaccc atagctaatc tttttcaact gatgaatgtg gtttcacaac
    15121 cagaaaccat tcagtttttc aaggaaaaat acaacgattg ctccattcgc tacggcgacc
    15181 tgaagaagca gttggccgaa gacatgatca cgttcgtcaa tccgttcagg gagcggatag
    15241 aagatatcat ggctgatgaa gcgttcctaa accgtgtagc tgaaatggga aaggaaaaag
    15301 ccaaagccag tgccggaaaa acaatccgtg aagtaagaga aataatcgga tttaagccgt
    15361 attaatcttt gagctcaatg gattttcaaa tacagtaaga aacaaatgca aaaagcgaaa
    15421 gatagaaatg tttttcgctt ttttgattct gaagcatcaa agtaaacggg ctcgaactgt
    15481 aatcttcaca tttcgagaaa agaaaaagaa ttacgaaaat catttcatac aaatcattgt
    15541 aaaatatgta accaggctaa attccaacct gttttcacaa agaaacaaaa aagcattgaa
    15601 aaataaatgc taactcaatg attttttgta gctttgcatt ccgaaaaaga catactgaaa
    15661 atggtaaaaa agcagattaa acgggcgctg atttcggttt atcacaaaga aagactggat
    15721 ctgataatca aaaaactgca tgatgaaggc gtttcattcc tttcaacagg cggaacaaaa
    15781 acttttatcg agtcgcaggg cattccttgc gaagccgtgg aagaactcac tacttatcca
    15841 tccattctgg gcggaagggt aaaaacactt catccgaaag tgtttggagg aattttagcc
    15901 cgcagggatg ttgaaaaaga tgctgaacaa ctcacagaat atcaaattcc ggaaattgac
    15961 ctggtaatag ttgacctata cccctttgaa gcaactgtag ctgccggagc tgatgaaaaa
    16021 aatgtaatag aaaaaattga tataggtggc atttcactca tacgtgctgc tgccaaaaac
    16081 tacaaggatg ttgtcatcct ggcgtcacag gatcaataca tgcctttttt agagttgatt
    16141 aatcaaaacg gagcagaaac cacacttgaa gaacgccgct ggtttgccaa agaagcattt
    16201 gcagtgactt cacactacga ttcggctatt ttcaactact tcgacggtga agatgcaagc
    16261 gctttccgat tcacagctaa tggctccaaa ccgcttcgtt acggcgaaaa tccacatcag
    16321 aaaggatact ttttcggaga attcaacgag atgttcgagc agcttcacgg aaaggaaata
    16381 tcatacaaca acctgctcga catcgatgca gccgttaacc ttatcaacga cttcgaagat
    16441 attacctttg ccattctgaa gcacaacaac gcctgcggac ttgcatcgcg cgatgaactg
    16501 aaaaccgcct ggaaagatgc ccttgccggc gatccggttt cggcttacgg aggagtgctc
    16561 attacaaatg ccattgtgga taaagatact gccgaagaaa taaacaagct gttttttgaa
    16621 gtaatcattg ctccggatta cgacctggat gctctcgaac tgcttacaca aaagaaaaac
    16681 cgcatcattc tggtgctgga aaataccaaa gttcctcaaa agcaatttcg ctcggtactc
    16741 aacggcgtat tggttcagga gagagataca tatcaggaaa ccggagaaga actgaaaacg
    16801 gtaacagaaa aagaaccgga tgaagatgaa atagatgacc tgctttttgc caataaaata
    16861 gtgaaacact ccaaatccaa cgcgattgtt ctagcaaaag acaaacaact gatagccagt
    16921 ggagtagggc agacttcgcg cgtggatgcc ctaaagcagg ctattgaaaa agccaaatca
    16981 tttgactttg atttgaaggg tgctgtgatg gcatcggatg catttttccc attcaacgat
    17041 tgcgtagaga ttgctgcaga agcaggaata actgccataa tacagcccgg aggttcggtg
    17101 agagacaccg acagcatcga aatggccaat cagaaaggag tatccatggt gacgaccgga
    17161 atcaggcatt tcaaacatta atattaaaat aaaaaaacaa cacattaaaa aatgattttg
    17221 aaatacgaat taccatttcg taactccaaa atcgtaaatc taaaataaga atatgggact
    17281 tttttcattt acacaagaga tagccatgga cctgggcacg gctaatacaa ttattataca
    17341 ggatgataaa atagtagtgg atgagccatc ggtagtggct ctggacaaac gaaccgacaa
    17401 actgatagct attggcgaaa aagcacgtca gatgcaggga aaggaaaacg aagggattcg
    17461 taccgttcgc cccttgcgcg acggtgtgat tgctgatttc tatgcctgcg aactgatgat
    17521 gcgcggcatg ataaagatga taccgcagaa aagccgtctt ttctctccaa cattgaaaat
    17581 ggtggtttgt atcccatccg gaagcactga agtggaaata cgtgcggtac gtgattcatc
    17641 cgaacatgca ggcggaagag atatttacat gatctacgaa cccatggcag ctgccatcgg
    17701 aattggtatc gacgtggaag cacccaatgg ctgtatgatt gttgacatcg gaggaggaac
    17761 taccgaaata gctgtaattt cgctgggagg tatcgtttcc aataaatcga ttcgtatagc
    17821 cggtgacgat cttactgcag atattatgga gtacatggga cgtatgcaca acattaaagt
    17881 gggtgaacgt acggccgaaa tgataaagat taacgtaggt gctgctctca cagatctgga
    17941 ggatgctccg gaagattatg tggttcacgg tccgaaccgt atgacagcat tgccaatgga
    18001 agtgccggtt tcgtatcagg aaattgctca ttgcctcgaa aaatcggtat caaagattga
    18061 agcagccgta ttgagcgcgt tggaacaaac acccccggaa ttgtattcgg atattgtgca
    18121 aagcggtgtt taccttgccg gcggcggagc attacttcgc ggactggata aacgactgac
    18181 agacaaactg aacatcaaat ttcatgtggc cgaagatccg ttgcatgctg tagcacgagg
    18241 taccggtatt gctctgaaga atgtggataa atatccattc ctgatgagat aaaaatcgga
    18301 acattgtttg tcattgttgc gattttagag gtctttttac ctcactgcaa aattttaaaa
    18361 aaataaacac acgattaaca gttgtttttc gtgtgttttt acaattattc atcgttttcg
    18421 cattttgaag aatcttatca gttttttgat caggtacagt gttgttttct tgtttattat
    18481 actggaaatc atatcattgg ttttgatatc ccgaaacaag ggctatcagc ggagcgtttt
    18541 gctttcttct accaataccg tggtcgggaa aatgtacgaa acgagcagca aagtggtcga
    18601 atttttcaag ctgagcaacg caaataaaaa tcttgccgaa gaaaatactt ttttgctcaa
    18661 cagagtaaca gaactggaaa atacgctcaa tacgcttaca gatactgtga acagtaacga
    18721 atggaaaacg gtgcgtatac ctcccgaacg gcagtacgac tatatagccg ccaaggtaat
    18781 tcgtatcacc acccatcagt tacaaaatta cataaccatc aacaaaggct cactggacag
    18841 cataaaaccc gatatgggag tggttggcga tcagggagta gttgggattg tagaagccgt
    18901 ttcgcctcat tttgctaaag tgatacccat tctgcaccca aaatcaacca ttgtgaccaa
    18961 attcaaaaaa agcaattatt ttggtccgct tgtgtgggat ggtcaggact atcggtatgc
    19021 caagctcaac gacattgccc gtcatgtgaa gttcagtctg ggagatacgc ttgtgtcgag
    19081 tggtttcaaa tcatttcccg aaggtatcat cgtaggcaca atcaataatt ttgacatcag
    19141 agaaagtgat gcatattaca acatacaggt aaggctggct gttgattttc gctcactgac
    19201 gcatgtgaaa gtaatcaact ataagaatta ccaggagcaa acagagctgg aaaagggaac
    19261 tccggatgaa aagacaattt ccaaaaaaga taaatagtag tcgttcatgc aattagttat
    19321 tcaaaacata tttcggtttg ttatgctggt attggttcag gtatttgtat tggacaatat
    19381 tcagtttctg ggctatgtaa gtcctatgat ttacgttttg tttatacttt cgctgcctgt
    19441 tcgtttcccc aaaggagttt tgttaattct ggcttttatt ctcggactta ctgttgatat
    19501 tttcaacaat acgatgggaa tacatgcatt tgcaactgtt tttgctgctt ttgtgcgccc
    19561 ggtagtcatt aatatgtcag tttcaacaga agaaatggca aatccatcac cttcattccg
    19621 aacttttgga gtggcaaatt acgtgaaata tgtcattcta atcgtacttt tccaccacat
    19681 tgtattattt ttagtcgagt cattttcatt tatgcatctc acgctgctcg tcccgaagat
    19741 attattaagt tctgcggtta ccattcttat tattttaggc attcagtcac tcaagtcgaa
    19801 ataatgatca acgataagta tcaaaaaaga agcactacaa tggcagttat catgattgtt
    19861 gttgtagtaa tctatatttt acgacttttc tcgcttcaga tactcgaaga caagtataag
    19921 aaaggagctg ccagcaatgc atttctgaat aaaaccattt atcctccacg cggactgctg
    19981 tatgaccgca acgactcgct ccttgtgtac aataaacctg cttacgatat tgcttttatc
    20041 aatcgtgaaa tacggaatct ggatactctt tcattctgta acgatctgaa aatcactaaa
    20101 gaatacttta tcgaaaggat gaaagaggtt aaaaacaggg ataaaaatcc gtcgtattct
    20161 tcgcttactc ctcaggtgtt tatgactcag ctggagatta aagatgtggc agctgtgcag
    20221 cagtccatgt acaagtacag cggattttat attcaaacac gtactctgcg cgaatacagg
    20281 tatgcagccg gtgggcatgt acttggtagt attggcgaaa ttacacccag aatgcttgag
    20341 aatgatgaag aaggatatta cgatccgggc gattatgtgg gtcgtgatgg atttgaattt
    20401 acctatgaga aagacttgag aggagaaaag ggagtggagg tattgctgcg cgatgcccgc
    20461 ggccggataa aggggaaata cgaggaagga agaaaagaca ttgcacccaa agccggtaaa
    20521 aacatcaaaa tgacgctgga tattaaactg caaatattgg gagagcagtt gctggcaggc
    20581 aaggtgggaa gtgtagtggc cattgaaccg aatacgggtg aaattcttgc catggtgtcc
    20641 aatcctacgt ttgatccttc gctgctcgtt ggtcgcgagc gttcaaaaaa ttactcgcta
    20701 ctggtcaatg atcctaccaa accgttgatg aatcgcgcta cacaggcgca gtattctccc
    20761 ggttcctcat tcaaaccgtt tcaggcactg gtggcattga agcagggcgc tatcaacgaa
    20821 ggaacaaggt tctcatgcaa tggtcccggt tcgtcgccca tccggtgtac acaccaccac
    20881 ggatctcccg tttcgttgct caatgccatc gaacaaagtt gtaatcctta tttctggcat
    20941 acttttgagt ccacgctggt aaaaggaggt tatggaaagg ataaccagaa tttccggaaa
    21001 aattacgatg aatggagaat aaatatcatg aaatttggct ttggagccag gtttgaagat
    21061 tcggatatct atcagcaaag caggggaaat attccttcgg aaaactattt tgacaaagtg
    21121 tataaaagca aaacgggttg gagagcgctt accattcgtt cgctgtcaat tgggcagggt
    21181 gaagtgctgg ttacgccgct tcagcttgct aatgccactt ctgtaatagc aaaccgggga
    21241 tttttcatca ctccgcattt gaacaaatca gactcaatgc tgcttcacaa gcacgatgta
    21301 ggagtagata aaaggcactt tcaggtagtg gatgaaggaa tgtggagagt aaatgaattc
    21361 gggactgcaa ggagttataa agtacccggc ataaactggt gtggaaaaac cggtacggtg
    21421 caaaataacc gcggaaaaga tcacgcattc tacatgggat acgctccgcg cgaaaatccg
    21481 aaaatagcaa tagctgtggt ggtggaaaat gcaggtttcg gctctacatt tgctgctccc
    21541 attgccagcc tgatggtaga acagtatctt accggaaaag tggtccgaac ggacttatta
    21601 actaaaacaa tgaatgtaag aacaaatgcc aacgtcaagg aatattagca taaaatatgc
    21661 gctggactgg aaaacggtat ttatttatct gtttctggta gcctttggct ggttgaatat
    21721 ctatggcgca agctatgatt ttgatcaggc cagtattttt gacttcagtc agcgggcagg
    21781 taaacagttt gtatggattc tgacggcttt cggcctggca ggtgtgctgc ttttacttga
    21841 ttcaagatta tacagttatt ttgcctatct catttatatc tttgtcatat ttctgctcat
    21901 agttactata tttattgcta ccgatgtaaa aggttcccga tcgtggctgg tattcggtcc
    21961 tgtaagtttt cagcccgccg aacttgccaa aactgctaca gccctggcgt tggctaaatt
    22021 catgagtgcc tacaactata cgctgaaagg ttggaaggat ctgattccac ttgcagtcat
    22081 tgtttttatc ccgtttgctt tgataattct tcagaaggaa acaggtagtg cgctggtatt
    22141 tctggcattt ttgctcatgt tttaccgcga aggaatgagt ggacttgtcc ttctgctggg
    22201 cgtatttgcg gtagctttat ttgtcatcgc tatcggattc agcgagatac cgatacagac
    22261 tgatagcggc acgctgggga tggtactggc tatgttactg gtcattttgg tacagtttgt
    22321 ttttgccttt ttttataagc gtaaccgcaa agaagctttg ttgctgctgg caggagtggc
    22381 tggaatgtcg atcattgcag ttgtgcttaa tatctggctt cccgtaaatt acgactacgt
    22441 ggcaattttt attgttttac tttcgtgtgt gtattggttt ataatttcca ggttcaaacg
    22501 aatgaaacaa tacctgttta tagcctggat ttctctggga gcaatgttgt tctgtttttc
    22561 ggccgattat ttgttttacc aggtattaga gccgcatcag caaatccgca ttaaagtact
    22621 cctgaatatg gaagaagacc tccgcggcgc aggttacaat gtcaatcagt ccaaaattgc
    22681 cattggttcc ggaggtatgt ttggtaaagg gtacctcaat ggtactcaaa ccaaacttaa
    22741 gtatgttccg gagcaggata ctgattttat tttttgtacc gtgggtgaag aatggggttt
    22801 tctgggttca tccgttcttt tgtttattta cctgtggctg cttctgagga ttatacggat
    22861 tgccgaaagg cagaatgagg tgttcaaccg agtgtacggc tattcggtag ccagtatatt
    22921 tttcttccat ctcactatca acgtggggat ggtactcggc ttggtacccg taatcggtat
    22981 tccgctgcca tttctcagct acggcggttc gtcgctctgg agttttaccg ttttactttt
    23041 tattctgctc aggctggatg cggcaagact tcagcgggga gtgcagcgtt agttttttgg
    23101 tttttatgta ttcaaatatt agccgatttt acctaaaatt gcaacatagt ttttgccgta
    23161 ttttttggca cacttggggc aggtagtata ccacatgtac attttttcga tttcaaggtt
    23221 tttacctttg gcataatccc gatagttttt tgtccacttt ccggtttcgc ggaaatctcc
    23281 ttcgtacacc ttgcacagga atttccccga aaaagttaca ttattaagtc ctgccacttc
    23341 gcggtccaca gccagacaga catccatttt ccaggcagaa gtatgatctg acagtcccat
    23401 tccgtcctgt tgcatggatg cacctgaatt tcgtattttc gtatccagtt tgcgcatcac
    23461 cgcaccaaag ttgagcggca tgtacatgaa tgtacagaca ctccctttta caaatttttt
    23521 atttttccat tccagtgttt tctcatccca gcgttcgggt tcaaacttcg ggcagcattc
    23581 cgtttcttca tttacaattg gttgtttact ttccatacag gtatagagtt ttagcttgta
    23641 atacactata tataaaacag taatgaataa atatttgttt ttttaaaaac taaattaaat
    23701 aaatctatct gtgtttttct tcaaaatata ttcataactt tggagggata aattcatgta
    23761 ttttcctaat tctaaagaac caaaaatgaa tggaaaacta attgcctgtt gtggtatcaa
    23821 ctgtgccacg tgcgatgcct gcattgctac catgacccat gatgaggagc tgcgtgccaa
    23881 aactgccgaa aaatggagtg tggaatataa ctcaagtgat attactactg aaacacttta
    23941 ttgtaccggt tgccgtcagg caggagctaa atttgcgtat tgtgaatttt gcgaaattcg
    24001 caactgtgtg aaatcgaagg gatttgaaac ctgtggagag tgtgatcagt tggagaactg
    24061 ctctatcgtg ggtaatgtac tgaaaattct acccgaagcg cttgacaatc ttaaatcgtt
    24121 gaactgattg ttcaaagtgc cgggttaagc attgaaaaaa caatcaatgc cgtttccatt
    24181 caatttcgga aacggcatcg tgttttttta caacttaaat tttacatcca cctgatacaa
    24241 tcggtagtgt ttacacacca atttgagcat taaataaaac caattttact caaaatctga
    24301 acatccggcg cgtgtcagaa atacgatttc acctggtaaa atcctctgtg aacatcttca
    24361 tataattttt ccagattttg cctgctcgtt attttatagg tttttttcct gaaaataaca
    24421 ataccgcaaa tttcttcgtc gtcacagttt ttgtcgaaaa aatctcctac attaacttcc
    24481 agaattcggg caatattttc aagtgtttct gttaatgaat tttccctgat gtttcctgcg
    24541 agttcttctt ccgttatgcc cagcttatat gcaatttctt tcagcgtata gccacttgcc
    24601 ctgattaatt cataaactct taacataata agattaattt gaaaaagtta aacatacaat
    24661 catcgccaaa tttcatgtaa taaagttgtt cggttcacct gctcacccgt atatactcaa
    24721 tcccgaaaat ttaactgtat atacctttat tcaaaaatta aacataaaag atttatggtt
    24781 ggttttcaaa gttttgctaa aagtgaaccg ttccttgttt aaaaaaatat aatcaggaaa
    24841 ttaacgcaga ccctggtttg actaaccaaa atatcgggtg atactgtact gaagcgttgt
    24901 tggctatgcg gtggttcgtt ccggttaagt taacgttgtg aaatgtagtt taattatctg
    24961 gaaatcaata ataactatta aaactgattt gtttaataaa cctttaacta tgaaatactt
    25021 tttgaaaaaa tcaggattat ttcgtttgca tttacaatga attcccggga aaatgatgaa
    25081 taaaaaagca gtgagaatca ccgatgaaaa cgacttttta aatgctcgag atgttgattc
    25141 ctgtgatttt tttatacaga tcgagtgcat atacgtccgt catacctgaa atataatcca
    25201 gcaccgcctg tacttttccg taagttgtag ggtttttgat gtcatattga gccggaaccc
    25261 gatcaagcaa aagttttgag tacgtttttt cgggatgaag cacggcattc acaaaaaatt
    25321 ccatcaactg atatataata cggtaaccgg ccagttcgat atccagcacc tcctgtgact
    25381 tgtaaatttt gtttacagcc acttcagagc agtttttata cgcttcagaa gtgtgaacgg
    25441 atacatgttt tatcaatgaa ccttcataat ccccgtttag aatagcttct tcgttattca
    25501 ggaaagcgct gctgcattca ttcaccagct gaccgataac cgaagaacgc aggtatgcaa
    25561 tttgttcgtt tacatcgctc accagcgaga gcgtttcccg gattcttttc aaccgttttt
    25621 catcaaagaa gcggaaatac atttctttgg tttcatcggt ggtaactatc ttcagtttgt
    25681 gcgcatcttc aatgtccatc acttgatagc agatatcatc ggctgcttcc accagatata
    25741 ccaacgggtg acgagcgaaa cggtccggat tgtttttctg acgtgaaatg ccgagttcaa
    25801 cggctatttt ttcataatcg ctccgttcag aatcgaagaa accaaacttt tgttttttct
    25861 tgtcggaatg aacagatgca taagggtact ttacgataga ggccaatgtg ctgtatgtga
    25921 gtacgaatcc gcctggtcgt ctgccgttga actggtgagt gagcaaccgg aacgcattgg
    25981 cattgccatc aaagcgggta aaatccgccc attccgtttc cgtcagttta tctttcagaa
    26041 aaatgccttc cccttctgaa aagtaagtag caatggcttc ttcacccgaa tgtccgaaag
    26101 gcggattgcc caggtcgtgt gccaggcagg cagccgaaac aattgccccg atttcatcga
    26161 agtaattcac ttgctcgttg cggtgagcct gagcaatatt cacgcccagt gaacgtccca
    26221 cgcttgccac ttccaggctg tgagtaagcc ggttgtgtac aaagatactt cccggaagcg
    26281 ggaatacctg tgttttgttc tggagtctgc ggaatggggc tgaaaatatc agccggtcat
    26341 aatcgcgctg aaactgatta cgattatcgc gttcgcggtc atggaaatct tccataccca
    26401 gacgcatggg cgagaggagt tgctgccagt tcattttacg tagagtacgt cctgataatc
    26461 cggtgtccgg tccatcattt tcactacgta gggacaaacc ggaatcactt ttaattcgtt
    26521 ttcacgggag aatttcacca tttcttccat cagatacaca ccgtatccga gccctttgaa
    26581 tgattcgtca atgcctgtat ggttaacgct gatgacattt ttttctgtga aaacaaatgt
    26641 cattttaccc gaagctaccc cgttcacaag cagcataaat tcaccgtgtt tgtcgttgtg
    26701 gtttgttttt acctgtatat ccatagtttt tttgatattt ttatagaaaa caatcttcaa
    26761 cctgtaaagt ttgaaaatag ctccactaca atgattttct ttttcgccaa atctgaagtg
    26821 aattgaaaat attctgccag atgacgtacg ctcccggacc caccgacgac aacgggagca
    26881 ggtatgtttg agccatccag atagccagaa cagtgttttt ctggcccaga ctctgtccac
    26941 cggctatggt ttcgccgtag gcttttccta tcagtttccc ggtgaaaaac tgtatcagac
    27001 aaacagccag tgtaccggct gccagcaaaa tttctgtagc atagctttct tttccctgca
    27061 tgatgatgaa attcaccgtt ctggctgtaa caataatgag tgcaaaagtc cagatgtaga
    27121 atgaaagtcc ggaaacggag ccgagacggc tgccgacttt gggtaaaagc cgcttcgtaa
    27181 tcatagcgag caccagcgga cccagcaaaa gcaatacaat gtgttcggaa atagttagaa
    27241 acgaagaaaa gaaaccgatt tccagtgtgt tgttagcata cgaaaacaac actggtgcca
    27301 gaataaccac catcaggttg ctcagtagca tatatgctgt caggttttcc acattccctt
    27361 tgagcatatg ggttataacc ggtgccgaag tggctgtggg aacgagcaca caaatcatga
    27421 ttccctgagc aagaatttca tttaccggac gcaataaaaa atagatagtt gtactcacaa
    27481 agatttggaa aaaaatcagc cagaagtgca tgcgtttcag ccggatgtga ctcagattta
    27541 atttgttata agtcagaaac aacattacaa aaatcaggta gggagtgagg aatgcgagcc
    27601 tgctgaagag cggataaagg gcagctcctg tcagcatggc aatgggcatc atgtacgatt
    27661 ttagtttttt cagcattctt tttttcaggc tagtttattt atatcgaaga tgtaaaaaat
    27721 ttctgtttat ggtttctttg gaagaaacag gtgccagtcg cgtccgtatg ttatccagtc
    27781 gaggttaaac cgtgcaaaaa tcccatcggt ggtcaggaag ataagtccgt cgcttggagt
    27841 ttttggtctt ccggctgcca ggtgcgaata accgccgtgt gggctgacca gtctttttac
    27901 aggccatgtt ttaccgccgt cgaaacttgc ccaaacggta atattaattc ttcctgccgt
    27961 actgtgaata gctgtaggcc cgtacggatt atcggtcatg ctgaaaacga gtatatcata
    28021 tccctctatt tccaggcgga cgagtccggc tttgttcccg taaacattcg gaggcccgtc
    28081 gggcaataca ggatctatgt actgatcaac ccatgtttcg ccaccgtcgt aactgaatgc
    28141 gatgtctctg tttcccggtc ggttgtggtt gcgcgagttg taataaacag taccgtcgct
    28201 cagttcaacc aatgcagctt cgccggtccc ggcaacaggg taaggtttgc ttgtaaacca
    28261 ggtttttccg ccatcgtcgg aataaattgc gctgctgtaa tgatatggca gcaggctgtc
    28321 gttacttgcg ttgtagtcta taagtacccg cgcaggtgcc agcaaccgtc ctttgtgttt
    28381 gccgtgacgg agtgtaattc cctcttcgca tccgctgatt atcggcagcc atccgttgat
    28441 atccggatgt ctaactattt cttcctttac ccaggtaaat ccactgtctt tacttctgta
    28501 catgaaattt tcaggttttc gtcccgctcc acccggatct aacttcagaa tatctccggt
    28561 tgtttcatcg agtactctcc ctgcacctcc cagcagaggt attctcgcgc tccaggtcac
    28621 acctccgtcc acacttattc tgtcggccaa caaagatcca tccattgcca ccatgatatc
    28681 accggtccat ccattcgcca tatttccgtt gtatatggga aacacttcat caatatcaat
    28741 aaattccctg gatgtagtga catttccgga tggtttttca ttttcacatg aaatgagact
    28801 cagcgaaacc agtgatgaaa ccaatatgta ttttatacat ttaatcattt cggaatgctg
    28861 tagtttttca attcggttct gtattttttt gcctttccat cggttacccg gtccatccat
    28921 tcccagaatc ggggactgtg attcatttct ttggtatgac agagttcgtg taagatgaca
    28981 tagtctgcca ggtattcagg caaaagcatc agatagaacg ataggttgat attctttttt
    29041 cggttgcagc ttccccagcg ggttttcgac gactggattt taacatcagt gtaagaaaat
    29101 ccatatttac cggctaacat ctgtgttcga tgcggaagaa ttcgttttgc ctccttgcgc
    29161 agaaaataat tcacggcatt ccaaaaataa gtttgagttt gtacgtcgtt aacggataaa
    29221 tgctgcggaa attcaatgtt aagaattccc gatttcaacg atgcatacag cgtgtttcgc
    29281 tctgctttcc ggatgtgaat atcgaatgtc tgagtagtaa gtgtttttcc ttcgttcagt
    29341 ttgattgtct ggctgctgat gcttttttgt cgtttgatca gctttaatcg gattttttca
    29401 atgaatttca acccgtcatt ttcgctgtat cctacaggca gatttacctg caatgaatcg
    29461 cttaatatcc gtatactcat gcgtcgtgca cggatattct gtttgaattc tatctttccg
    29521 aggtctttgt cctgaatcac gggtgtttct attgtcgtag tttttactgc aaagttacaa
    29581 ttttttcggc cccgtttgca tgataataag cagaaacggc taactttgca ttaaataaaa
    29641 caaaacgacc gcaaatgata catgcaacga acatacataa aagttttggt gaactagaag
    29701 tgctaaaagg ggttgacctc gtggtgaata agggggaaat tgtatcgatt gtaggtccga
    29761 gtggtgccgg aaaaaccact ttgttacaga ttctcggaac actggataag cccgactcgg
    29821 gcaatgtgat gattgacgga ctggatgtaa gtaagctaaa ctcagaccgt atttcagaat
    29881 tccggaacaa gcacgtcggt tttatttttc aatttcacca gctgcttccc gaatttaccg
    29941 ctctcgaaaa tgtgacaatc cccgcactca tcggccgtac cgatttcaga aaagccactc
    30001 agcgcgccaa agagctgctt gattttctac agttgtccga ccggctcgaa cataagccgg
    30061 cagaactttc gggaggtgaa aagcagcgtg tagcggtggc tcgcgcactg attaacaatc
    30121 cgtcgctgat actagctgat gaaccttcgg gcagcctgga tactcaaaat aaggaggaat
    30181 tgcacaaact cctttttcaa ttgcgtgata aattcggcct tactgaagtt atcgtcactc
    30241 acgatcatga tctggcaaaa atctgtgacc gaaccatcga aatgaaagac ggacggatcg
    30301 gggggtgaaa ag
//
LOCUS       DAORKP010000005        29813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_30_length_29813_cov_8.030647, whole genome shotgun sequence.
ACCESSION   DAORKP010000005 DAORKP010000000
VERSION     DAORKP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29813
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30_length_29813_cov_8.030647"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..2644)
                     /locus_tag="PLN75_00675"
     CDS             complement(191..2644)
                     /locus_tag="PLN75_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRG02660.1"
                     /translation="MAPKKPVKNTSTQRKPSQAKTYRSTNRTGFFKRFFSFLKDERTH
                     FILGMFLGVIVLYTLLSFISYYFTGAADKSVFDNISFSESLSIRGSVKNWASVMGAFL
                     SETLIDNWFGVSSIAILFFLAILALWLMKVRFISVWKAFFHSFFWLVWVSVFFGYVTD
                     FLPSIQPSFFALGGKHGNYVALEMLNSYIGRPGTLMLLAATLIIYMVFTWGGTILFLK
                     NLFSRKKTANVPKYESNEPVVAVGTEIIPEDWIVGSDREFRVSDHFTDKNETAEPENE
                     VETESVTFEVTKPKTAEDQIDEYEAEENDDPNYNVSKLGKYDPTLDLSKYVPPTLDLL
                     KVYGSDKDLQVDMAEQTANKNRIISTLKNFGIDIDSIKATVGPTITLYEIVPKSGIRI
                     SKIRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNEPQIVSMHSVIGSKKFLESK
                     FELPVAFGKTITNEVYMIDLTKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPAQLKF
                     VLIDPKKVEFNIYGAIEKHFLAKLPDGEDAIITDTSKVVQTLNSLCMEMDDRYDLLKK
                     AHVRNIKEYNEKFVNRELNPEKGHRFLPYIVVIVDEFGDLIMTAGKEVEMPIARIAQL
                     ARAVGMHMVIATQRPSVNIITGVIKANFPARVAFRVSSMIDSRTILDAPGANQLVGRG
                     DMLFSQGSDLSRVQCAFVDTPEVEEIARFIGQQRGYPTAFYLPEYVSPEGEALDTASI
                     DMSKRDPMFEEAARMIVATQQGSTSMIQRKFSIGYNRAGRIADQLEVAGIIGPNEGSK
                     ARQVLVQTEYELENILKHL"
     gene            2775..3428
                     /gene="nth"
                     /locus_tag="PLN75_00680"
     CDS             2775..3428
                     /gene="nth"
                     /locus_tag="PLN75_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRG02661.1"
                     /translation="MTKKERFSNILAWFEANRPVAETELMYKDAFGLLVAVILSAQCT
                     DKRVNMTTPKLLQDYPTPEAMAASTPEVIFEYIRSISYPNNKSKHLVGMAQKLISDFN
                     GIVPDNVDDLQKLPGVGRKTANVIASVVYDKPAMAVDTHVFRVSERLGLTTDSKNPLE
                     TERELVKHIPAEIIPKAHHWLILHGRYVCIARKPKCEECGLKAWCRYYSKNAAKTDK"
     gene            3570..4076
                     /locus_tag="PLN75_00685"
     CDS             3570..4076
                     /locus_tag="PLN75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRG02662.1"
                     /translation="MNSNLMNSTMRHGLILGIVFSVNFILSISGNMFFGLLSYVAVGL
                     IIYLTYKYTISYRDTDCAGTISYRHALSFVILLFVFAALISAIVKYVYFQYINTDFLP
                     ELMNKSIELMEQIMPSVPEETYDNMEKMMSPINYSLTVTWMNVILGFLVGLVIAGLVK
                     KEKDLFEK"
     gene            4129..5082
                     /locus_tag="PLN75_00690"
     CDS             4129..5082
                     /locus_tag="PLN75_00690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG02663.1"
                     /translation="MDISVVVPLYNEEESLPKLYEWIERVMAENEFSYEVIFIDDGST
                     DGSWSIIEDMQKLNPAKIRGIKFRHNYGKSPALYRGFDAANGDVVITMDADLQDSPDE
                     IPELYHMIISGNYDLVSGWKKKRYDSKLTKNLPSKLYNATARRVTGLRLHDMNCGLKA
                     YRNDVVKNIEVFGDMHRYIPYLAKNAGFTRIGEKVVTHRKREFGESKFGMSRFVNGYL
                     DLMTLWFLSRFGKKPMHFFGTMGSLMFILGFIAVVIVGAHKLYALSNHIAAPLVVDSP
                     YFYLSLVLMILGTLLFLAGFVAEIVTRNSPERNNYKIEKEI"
     gene            5099..5473
                     /gene="crcB"
                     /locus_tag="PLN75_00695"
     CDS             5099..5473
                     /gene="crcB"
                     /locus_tag="PLN75_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRG02664.1"
                     /translation="MKQLLLVGFGGFFGSIARYWMSKLNLTWDFHSIPMGTLAVNIIG
                     SFLIGFMLGIFMNSNLLHTNLKLFLVVGFCGGFTTFSSFTNENFLLLQNGQYLTALIY
                     IGGSIIIGILAVFAGFLIANQL"
     gene            5478..5825
                     /locus_tag="PLN75_00700"
     CDS             5478..5825
                     /locus_tag="PLN75_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HRG02665.1"
                     /translation="METEYSVLKIFMSNTDKSGLDLLYEKIVKEAQNQGISGVTVFRG
                     IMGYGLSSTRIVNSKFWEISEKLPVIIEMTDKTEKLNDFYHSMEEKLKSTGKGCLVYI
                     QPINILLHQSGKK"
     gene            5832..6341
                     /locus_tag="PLN75_00705"
     CDS             5832..6341
                     /locus_tag="PLN75_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02666.1"
                     /translation="MEILKYTIPALLVVLVVYILLYQLFKMERQARETELKKTALPLI
                     TSTRLRAYERLTLLLERINPNNLLINSTAPDQTCLELQSELLATIRREFEHNTSQQIY
                     VSELLWQEIDEARENLIQLINTCSAQCKADEPATKLANIIVEVFNTPEDTALNAALRM
                     LKDEVKKLL"
     gene            6338..6832
                     /locus_tag="PLN75_00710"
     CDS             6338..6832
                     /locus_tag="PLN75_00710"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HRG02667.1"
                     /translation="MTKVKFKIPILTILLISAFIFSCEKDETCRKSRIVETGAGFYLD
                     SVNTTTGKRVLVKSTIDSLWVSGIGVDSLLYKKTTTNAIKLPLNQFAEESKFRMKTNK
                     VVDTITIRYKNKIEYLSLECGSIRTHTIDTVLTSGHYIRSVAIINPTVNTLSVENIQL
                     FHTK"
     gene            6801..7487
                     /locus_tag="PLN75_00715"
     CDS             6801..7487
                     /locus_tag="PLN75_00715"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HRG02668.1"
                     /translation="MKTYSYFILSSILLFCVTVQSTAQTKESVKKDSIPFLQGIWIEA
                     DVAPVFERALINKNAYRAQGNIQVNLKNKYYPVIEFGYAGADKTASNGIEFSTSGFFG
                     KAGADLSMLKPKPNSTQKNNNFLVGLRLGASRFNYSLYNQTITDNYWGNSETFNLESI
                     SSTKLWYEIAAGMRVEVFRNIYMGWTIRNKHLISKTESGQTTPWYIPGYGIGNTSVWG
                     LSYSIGYRIK"
     gene            7528..9549
                     /locus_tag="PLN75_00720"
     CDS             7528..9549
                     /locus_tag="PLN75_00720"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRG02669.1"
                     /translation="MNTTHELNKAADNIRILSAAMVEKANSGHPGGAMGGADFINVLY
                     SEFLEYDPADPHWDARDRFFLDPGHMSPMLYSVLALTGKYTMEDLKQFRQWESITPGH
                     PELDVARGVENTSGPLGQGHTYAVGAAIAAKFLKARFGEVMNHTIYTYISDGGIQEEV
                     SQGAGRIAGHLGLDNLIMFYDSNNVQLSTYTNSVTSEDVAMKYKAWGWKVMEINGNDV
                     TEIRTALKEAKKEKEKPTLIIGNTIMGRGAVTDEGQSYENMVDTHGQPLGATKASFAA
                     TIRNLGGDSENPFVIFDETKALYAKREAQLREIAEEKKAARQVWADENPEMAARLNQF
                     FSGKLPELDWKSIEQKPNQATRAASATVLTFLAENVENMIVSSADLSNSDKTDGFLKK
                     TKALQKNDFSGKFLQAGVAELTMACLCNGIALHGGVIAACGTFFVFSDYMKPAVRMAA
                     LMQLPVKYIWTHDAFRVGEDGPTHEPVEQEAQIRLMEKLKNHHGKNSVLVLRPADVEE
                     TTVSWKMALENTDTPTALILSRQNITNLPAKDRKTAAYEAEKGAYVVECDGNPDVVLL
                     ASGSEVATLAEGAALLRNDGMKVRIVSVPSEGLFRSQPQAYQESILPKDIKRFGLTAG
                     LPVNLEGLVGENGKVFGLESFGFSASYKVLDEKLGFTAENVYKQVKALF"
     gene            9736..10329
                     /locus_tag="PLN75_00725"
     CDS             9736..10329
                     /locus_tag="PLN75_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRG02670.1"
                     /translation="MDFITILLIAVGLSMDSFAVSISRGMCTQKLKFSQAFLLAAIFA
                     VFQGLMPLIGFGAGQVFADQIRAIDHWLAFAILLLIGIKMIFESVKPDKVNETKRNCN
                     NECNCDNKNFSLKYLVILAVATSIDALATGLIFTPYPDRILMAVSLIGITTFVFSYAG
                     VNLGHKFRHGFKFNFETAGGIVLIIIGLKILIEHLNA"
     gene            complement(10397..11857)
                     /gene="gltA"
                     /locus_tag="PLN75_00730"
     CDS             complement(10397..11857)
                     /gene="gltA"
                     /locus_tag="PLN75_00730"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRG02671.1"
                     /translation="MTKRDDTWRVELRAQMKAKERTNLHRVEMPELDSEYRSHIRNEE
                     VNLGLTPQMAIQEAHRCLDCANPTCMDGCPVNINIPDFIKNIERGEFLEAAKVLKETS
                     ALPAVCGRVCPQERQCEAQCIHVKMNKESVAIGHLERFAADYERESGQISVPETAPAN
                     GMKVAVVGSGPAGLSFAGDMAKLGYEVHVFEALHEIGGVLKYGIPEFRLPNHIVDVEI
                     DNLRQMGVRFMNNCIIGKTIAIHELEQDGFQGIFVASGAGLPRFMNIKGENLVGVMSS
                     NEYLTRVNLMDAANPDSDTPVYKGKKVAVIGGGNTAMDSVRTARRLGAEKAIIVYRRS
                     EEEMPARREEVIHAKSEGIDFMTLHNPIEYIGNESGRVTHMVLQVMQLGEPDESGRRS
                     PVEVPGKTVTVEVDEVIVSVGVSPNPLLAQSTPELQTSKRGTLVVNEDTLQSVSMPVI
                     FAGGDIVRGGATVILAMGDGRRAAKAMDEWIKSKLK"
     gene            complement(11854..12681)
                     /locus_tag="PLN75_00735"
     CDS             complement(11854..12681)
                     /locus_tag="PLN75_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRG02672.1"
                     /translation="MNKIISKENFSANVVKFVVEAPLIAKARRAGHFVMVRVGEKGER
                     IPLTIADADVERGTITLVIQRTGVSSTKICALEAGDYITDMVGPLGKATHIENFGTVV
                     CACGGVGTAPMMPIVAALKKAGNRVITVLAARTKDLIILEEQMRENSDEVIIMTDDGS
                     YGNKGLVTNGVESVIQSEKVDMCVTIGPAIMMKFVSELTRKYNVPTVASLNTIMVDGT
                     GMCGACRVTIGGKTRFVCVDGPEFDAHQVDFDEMLMRLGGYREQEKEEMERKVLHKK"
     gene            complement(12727..15162)
                     /locus_tag="PLN75_00740"
     CDS             complement(12727..15162)
                     /locus_tag="PLN75_00740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRG02673.1"
                     /translation="MEFTEIKRFIKRIFNVLKNWLFLFIEWRERRILRFKNLPTWRKV
                     LNTFLTIVFLFAFYLLLVDINFLYLYGKSPSIKAIANPPQSYSSEIISSDGKLIGKYF
                     TENRKEVTLSEISDEMINTLISTEDKRFYKHFGIDFRGLASAARDMLRGNPRGASTIT
                     QQLVKNMFKTRSQYSRGLLGSLPGLRLPIEKTKEWSAALKLELLYSKNEILQMYLNTV
                     SFGSNAYGIHTAAGTYFNTTPDKLNYEQSAVLVGILKAITAYNPYLNPDRSKQRRNVV
                     IGQLAQNNYISSNEADSLKQLPIKLNYKVEKPNEGVAPYFRSFLTKFLERWGETNGYD
                     IYADGLKIYVTIDSKMQEYAEEAVHKQMKTLQRNFFDHWSGQNPWRDENGKELTNFIP
                     EITKRSSVYKSLSVQFNGDEDSIQKYLNQPRKMKIFDYKEGTKEVTMSSMDSIRLMNH
                     FLHCGFVAMEPDTKQVKAWVGDIDYNYWHYDKVAQSKRQPGSTFKLFVYTAGMIAGKS
                     PCDRYQDKPFTWGKGKYAWQPKNANRSYSYNNLTLKRAFARSVNTVAAQLAKDVGIQN
                     VAGAARLLGIKSKLEEVPTLSLGASDVSLLEMVNSYSTVTHEGMYMAPVIVTKIVDKD
                     GYVIYEHKARPKRVISYENAFLMTELLKAGITEPGGTSRALWNYDLMRWDTEFGGKTG
                     TSSNYSDAWYIGVTPKLVAGSWVGAEHRSIHFRSGSMGQGSRTALPVFALFMEKVLAD
                     QKFSKYRAKFPSEPKEKISRDYKCESDYSAAYDGESNDSIFERVPIEALPIPDLKISN
                     EETEPIQTDPETP"
     gene            complement(15502..16782)
                     /gene="metK"
                     /locus_tag="PLN75_00745"
     CDS             complement(15502..16782)
                     /gene="metK"
                     /locus_tag="PLN75_00745"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRG02674.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDAILDNFLAQDANSKVACETL
                     VTTGQVVVAGEVRSQAYVDIQTIARKVINRIGYNKSEYMFDGNSCGIFSALHEQSKDI
                     NRGVEREEPMNQGAGDQGMMFGYACTETVNYLPVSLDLSHALVKELKVIRNEGKLMTY
                     LRPDSKSQVTIEYDENNKPLRVHTIVISTQHDDFIHPSDQRGQERADNEMLSIIKEDV
                     RNILIPRVKAQVPQFARLIKDDYVLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYAARHIAKNLVAAGVSDEVLVQVAYAIGLANPMNIYVN
                     TYGKSNVNLTDVQIAEKVAVLFDMRPKAIEKRLKLRNPIYEETASYGHVGRTPKKVEK
                     TFIDASGKEFKKNVELFTWEKLDMVDEIKKEFGL"
     gene            complement(17046..17639)
                     /locus_tag="PLN75_00750"
     CDS             complement(17046..17639)
                     /locus_tag="PLN75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRG02675.1"
                     /translation="MKNLIIIVIMALAIAGCSSVKKNGESEKNANQVALNSTTQTFYD
                     FSALNMQGKEIKLSDYKGKIVLVVNTASKCGFTPQFAGLEELYKKYSSRGFVVLGFPS
                     NQFANQDPGSNEEILEFCKANYGVTFPMFEKIDVNGENAHPVYTYLKNSVPNAEPEKI
                     KWNFTKFLIGKDGKTLKRYESRVKPADIEADIQELLK"
     gene            17763..18533
                     /locus_tag="PLN75_00755"
     CDS             17763..18533
                     /locus_tag="PLN75_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRG02676.1"
                     /translation="MLGAIIGDIAGSRFEFNGFKSTRFELFDPACDYTDDSICTVAIA
                     DAVLNKKSYQDSLLEWCRKYPNPMGSYGNRFSAWIKSENPQPYGSFGNGSAMRVSAIG
                     WLFEDEVETLRQAALSAIPTHNHPEGIKGAQAVALAIFRSRNGADKSEIIKETEQKFG
                     YNLQLTCDEIRPAYCYSETCQDTVPQAITAFLESTCFEDAVRLAVSLGGDADTLTAIT
                     GSIAEAFYKEIPSVISKKALEYLPKDMKSVINQFYRSI"
     regulatory      18651..18745
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18808..21222
                     /locus_tag="PLN75_00760"
     CDS             18808..21222
                     /locus_tag="PLN75_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG02677.1"
                     /translation="MRRHFLFTLVLISTLAVQSQNFIRGKVLDDNNQPIRGAYVILKQ
                     TSRTVISDPKGEFYFGNLSDFAYTLQVKCIGYEDFQKNVQTGSDIQIVLTHISINLEE
                     VNILATRATDRSPVAFSNVTKQKIEERNSGQDLPYLLAFTPSYITTSDAGTGIGYTGF
                     RIRGSDANRTNVTVNGIPLNDSESHSVFFVNMPDFASSLHSVQVQRGVGTSSNGPAAF
                     GASINMQTEVLNNKPYAEAGITVGSFNTLKNSVMAGTGMINNFAVDVRLSNIISDGYI
                     DRASVDMKSYFISGGYYGENTLVKFITFGGKEKTYQAWNGVDLENHTRTYNELGMYTD
                     NKNETKFYDNQTDNYNQTHYQLHFTQVINAGMNLNASLHYTRGKGYYEDYKTDRKYVE
                     YGLTPATVNGSILRKTDLIRQKWLDNHFGGATVSLNRKYEKLSLTAGGAANKYFGDHY
                     GKVIWVRYPNNAAPGHTYYFNESTKTDANIYAKLNYTITDRLHAYADFQYRFIEHKMS
                     GEDDKYDADKGSMRDITQSHIFHFFNPKGGLNYQINKSGSLYASFAIANREPNRNNYT
                     DAGPNEVPESERLYDTELGYRYLSNNLSLGANLYYMKYKGQLILTGKISDIGEMLTSN
                     IPDSYRAGVELMAGLKITEGLRWDGNLNLSSNKIKNFREYADEYDAEWNRTGTKENFF
                     KTTNISFSPNSVANSILSYSFKGIETAFYSTFVGRQYLDNTSDVNRSIDAYFVSNLRL
                     GYSWKMKKIKSVDFNLLINNLFDTDYETNGYNWYTYYLAGERINEKRYFPQAGIHFLF
                     STAVKL"
     gene            complement(21296..22960)
                     /locus_tag="PLN75_00765"
     CDS             complement(21296..22960)
                     /locus_tag="PLN75_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG02678.1"
                     /translation="MHKKIVPTPLTLKSLIDYSVSQFADCTSVSFVDGKPITYKELGE
                     KIDEFAALLFSSGLKKGDKVALFSHNMPNWVIAYFSVVSRGLIIVPVLPDFTTEEVEN
                     VLTHSESKVLIVSERLFPKVSQIETPDLIVKIKLDDFTFLSDISKLTFDSNKITETDV
                     TEDDVAAIIYTSGTTGRSKGVVLKHKSVTFVAMQSYTVQPINHNDVFLSFLPLSHTYE
                     NSIGMLYPIMYGATIYFLEKLPTAPVLIAAMEKIKPTMMLSVPLIMEKLYKNQIYLKF
                     RTSMLKKLVYRTPVTRKIIHHIAGKKLYKTFGGRLKFFGIGGAKLDYYVERFLKEAKF
                     PYAIGYGLTETAPLLAGAAPHQTKLQSTGYALEGVELRINEPDELGVGEIWAKGPNVM
                     LEYYKNPEATAEVLTTDKWFRTGDLGQFDKKKRLFIKGRLKNTIIGASGENIYPEDIE
                     SIINNTAYVSESLVVEEDGFLVAKILLDLEMAEKNIEHIKLIIEEKKEHYHDWKNQLK
                     KDINSKLGRSSQINRVDIMEEPFEKTASQKIKRFKYQKTKSKHDKK"
     gene            complement(23059..23811)
                     /locus_tag="PLN75_00770"
     CDS             complement(23059..23811)
                     /locus_tag="PLN75_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRG02679.1"
                     /translation="MNNNSKIQDSHISPLRGAGGLYLIPTTLGDTETDKILPRGNADI
                     ITSIRYFIVENIRTARRFLKKINRDIIIDELTFFELNEHTKPEEIAQFLQPLKTGNDM
                     GVISEAGCPAIADPGADVVALAQQQNFRVVPLVGPSSILLSLMASGFNGQSFAFLGYL
                     PVQQSERIKALKKMESRIYSENQTQIFIETPYRNMKMLDDILTACNPATKLCIAADIT
                     LETEFIHTKTVSAWKSQKPDLNKKPCIFLIYK"
     gene            complement(23862..25874)
                     /locus_tag="PLN75_00775"
     CDS             complement(23862..25874)
                     /locus_tag="PLN75_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRG02680.1"
                     /translation="MISIEQVTVEFGGSPLFDNIGFLINPKDKIALVGKNGAGKTTLL
                     KLIAGKQNPTRGNIVISPDLTIGYLPQNMVHNEGTSVLQEAEKAFEHITQVQQDIEKY
                     AGELASRTDYDSDEYLRIIEKHSRASEHYRIINPGNFHAEIEKTLMGLGFLRKDFDRQ
                     CSEFSGGWRMRIELAKILLQDPDILLLDEPTNHLDIESIQWLENFLVNSASAVLLVSH
                     DRAFIDAVTNRTIEISLGRIYDYNVNYSKFVELRKERHEQQVRAYENQQKMIQDTEDF
                     IERFRYKATKSVQVQSRIKQLEKLVRIEVDLEDTSRLSLKFPPSPRSGSIPVEIENLS
                     KAYGNHLVLDKIGMIINRGEKVSFVGKNGEGKSTLVKCIMDEIGYSGTMKLGHNVKIG
                     YFAQNQASLLDENLTVFQTIDYVATGDIRTRIRDILGAFMFGGEASDKKVKVLSGGER
                     SRLAMIRLLLEPVNLLILDEPTNHLDMPSKDVLKDAIRAFDGTAIIVSHDREFLDGLV
                     TKVYEFADKKVREHLGGIYDFLRKKNIEHLNQLGSPVTQPLKGSPLNSSQGGFLKNTK
                     VNNPENDENTGIISPSGGKEAYEARKELSKKIRKAEKDVETAENAVEKLEKEISEMLT
                     QLESPAKASDPEFIMLLQKKQRELEQKMYEWEILAEELEKLKTSEQ"
     gene            complement(25894..26988)
                     /locus_tag="PLN75_00780"
     CDS             complement(25894..26988)
                     /locus_tag="PLN75_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02681.1"
                     /translation="MDKIIPYIHKFPFLVVLVSFAIGYFFMPVVIRIAKNYNFVVSPN
                     KRTSHSGEIPNVGGVNIFVSFLITVFLFSFEVFYSVQFTLLGLFIILIVGFIDDLINI
                     KVSSKLFGELLAAFFLIVIADIRLSNLHGFLGIYEIPLIFSYFLSFFVFIVIVNALNL
                     IDGIDGLASGLGILYSLFFAVYFQLIGHPDLSITAFAMTGSLIVFFYYNVFSDKNKIF
                     MGDSGSLLLGYIFNVFIFSFCEINASGDLPEKYQISAAPAVAFTVLIVPLFDTIRVMI
                     TRIKKGYSPFRADRNHVHHLMLSLGLKHRQVTFILMGISAVFIILAVLGRNLSNGVLA
                     LVVLIICSLLTKILWTLVDKNKKNTNLSNK"
     gene            complement(26988..28937)
                     /locus_tag="PLN75_00785"
     CDS             complement(26988..28937)
                     /locus_tag="PLN75_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRG02682.1"
                     /translation="MEKNGSHSLFKSDIFSHLISFKYLPRWVVLAVDVAASIISYWFS
                     DLIASKLSIISNTTQLPLGLELILVISVQLFFFWLFHTYAGILRYSGFVDAIKLLFAV
                     FSTAGVLIVFNLISYFGFGHQLFYYLSILFYAVTAFFLLFLMRLIVKTVHDTISSNLG
                     NTTPVMIFGTKSAGTSIAKMLRSNERIKYKLVGFIDTDKEVTSKMLMGVKVYELTEES
                     VKKIVRKARAVIVSPLKMEEINPQTDLDIFINHGLAVLTVPSMSVWEGETLSPRQIKS
                     IQIEDLLERPQIKISTESISKELKNKVVLITGAAGSIGSEIVKQSIAFSPKLIVVMDQ
                     AETPMHEIILKLKEKYPNQNFSAFLGDVRNNERMKLMMDTYRPDIVFHAAAYKHVPLM
                     EDNPTECIQTNVMGTKNMADLAVKYNVSRFVMVSTDKAVNPTNVMGASKRIAEIYVQS
                     LNKKMFSENENTTKFITTRFGNVLGSNGSVIPYFKRQIENGGPVTVTHPEIIRYFMTI
                     PEACLLVLEAGTAGNGGEIFIFDMGEPVKILNLAKKMIMLAGFKPEVDIPIVFTGLRP
                     GEKLYEELLNKKEITKETHHPKIMVATVREYDYDEVSVKIDELINYSYRGKNYLTVSQ
                     MKSIVPEFVSNNSQYEKLDIEKMQN"
     gene            complement(29094..29813)
                     /locus_tag="PLN75_00790"
     CDS             complement(29094..29813)
                     /locus_tag="PLN75_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRG02683.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAVREGGPEADTN
                     PRLRVLIQQAKKENMPKENVERAIKKASSREESDYKEMVYEGYGPHGIAVVVETATDN
                     PTRTVGNIRSYFNKHGGSLGTSGSVLFMFDHNCVFHVAKKENLATDELELELIDYGAN
                     EIEEDEDEIIIYGEFASYSTIQKYLEENGFEIHSAEFERFPNDVKSLTEEQRASVNKL
                     LEKIEDDEDVQNVFHNMQLED"
BASE COUNT         8629 a         6344 c         6067 g         8773 t
ORIGIN      
        1 aaatgattat ccatgcaatc aacaggctga ttattaattt tacatctttc attttgatct
       61 gtcatttaat ttataaaata gttgtatctg ttaatttgca acttgcatgc caaagttttt
      121 gagcggttgg actaacttca ttccaaccgc ttaatatact ggccattgaa aaaatgataa
      181 gaaatttgga ttaaagatgc ttcagaatgt tttccagctc atattctgtc tgaacgagta
      241 cctggcgggc tttacttcct tcattcggtc cgatgattcc ggccacttcc agctggtcgg
      301 ctattcgtcc ggcacgattg taaccgatgg aaaattttcg ctgaatcatg gaggtcgagc
      361 cctgctgtgt ggctacaatc attcgggcgg cttcttcaaa cattggatcg cgtttgctca
      421 tatcaatgga agcggtatca agcgcttcac cttcgggcga aacatattcc ggcagataga
      481 atgctgttgg atagcctctt tgctgcccga taaacctggc aatttcttcc acttcgggcg
      541 tatccacaaa ggcacattgc acgcggctca ggtcgcttcc ctgagagaaa agcatatcgc
      601 cgcgtccgac cagctgattg gctcccggag catccagaat ggtgcgtgag tcgatcatgg
      661 acgatacgcg aaaagccacg cgagccggga aattggcctt aattacacct gtaataatat
      721 ttaccgaagg gcgctgggtg gcaataacca tgtgcatacc tacggcacgg gcaagctgag
      781 caatacgtgc aatgggcatt tccacctctt taccggctgt cattatcaga tcgccaaact
      841 catccactat gaccacaatg tatggcagaa accgatgtcc tttttccgga ttcagttcgc
      901 ggttcacaaa tttctcgttg tactctttga tattacgtac atgcgctttt ttcaataaat
      961 cataccggtc atccatttcc atgcacaacg aattgagtgt ctgaaccact ttgctggtgt
     1021 cggtaatgat tgcatcctca ccatcgggca gtttggccag aaagtgtttt tcgatggctc
     1081 cgtaaatgtt gaattccacc tttttgggat caatcagcac aaatttcagc tgagcagggt
     1141 gttttttata aagcaatgat gtgattatgg cattcagacc caccgatttt ccctgtccgg
     1201 ttgctcctgc taccagtagg tgaggcattt tggtcagatc aatcatgtac acctcattcg
     1261 taatcgtttt tccaaatgct acaggtagtt caaattttga ctcgaggaat ttcttcgaac
     1321 cgataacaga atgcatggag actatctgag gttcgttatt cggcacctcg ataccgattg
     1381 ttccctttcc gggtatcggt gcgatgatac gtatgccggt ggctgccagg ctcagcatga
     1441 tatccgattc caggtttcgg attttggaaa tccgaatgcc cgatttggga acgatttcgt
     1501 agagcgtaat agtaggtcca acggtagctt tgatggaatc aatatcgatt ccgaaatttt
     1561 tcaacgtgct gataatccgg tttttattgg ctgtttgttc ggccatatcc acctgcaaat
     1621 ctttgtccga gccatatact ttgagcaaat cgagtgtggg tggaacgtat ttcgacaaat
     1681 cgagcgtagg gtcatatttt ccaagtttcg atacattgta attcgggtca tcgttttctt
     1741 ctgcttcata ttcatctatc tgatcttcag cggtttttgg tttcgtcact tcgaaagtca
     1801 ccgattcggt ctcaacttca ttttcaggtt ctgcagtttc gtttttgtcg gtaaaatggt
     1861 cggaaactct gaattccctg tctgagccca caatccagtc ttcgggaata atttcagtgc
     1921 ccacagccac cacaggttca tttgattcat atttgggaac gttcgcagtc ttttttcgag
     1981 agaataaatt tttaaggaat aaaatggtac ctccccacgt aaaaaccatg taaatgatta
     2041 aagttgcagc caaaagcatc agcgtgccgg gtctgcctat atacgaattc agcatttcca
     2101 aagctacgta attgccgtgt ttaccgccta aggcaaaaaa agagggctga atggaaggaa
     2161 gaaaatcggt aacatatcca aagaaaacag aaacccacac cagccagaag aatgaatgaa
     2221 aaaatgcttt ccataccgat atgaacctca ccttcatgag ccacaaggca aggatggcca
     2281 gaaaaaacag tatggctatg gaagatacgc caaaccagtt gtcaataagt gtttcggaga
     2341 gaaaggcacc catcaccgaa gcccagtttt tcaccgaacc gcggatagaa agcgattcgc
     2401 tgaaacttat attatcaaaa acacttttat cggctgcacc ggtaaagtaa taggaaataa
     2461 aggaaagtaa agtatataaa acgatgactc ccagaaacat accgaggatg aaatgagtcc
     2521 tttcatcttt caaaaaagaa aaaaatctct tgaaaaatcc ggttctgttt gtcgaacgat
     2581 atgttttagc ttgtgaaggc tttctttgag tggatgtatt ttttacaggt tttttgggag
     2641 ccatttttac aggttttaag ctatttcaga ggacagaaaa cagatgtcaa cagtccaaat
     2701 taatttgcaa atttaataaa aatgaatggc aatgaaaacg gttttgatta aatttgcagg
     2761 aaaaaaaaat cggtatgaca aaaaaagaaa gattcagtaa tattcttgcc tggtttgaag
     2821 caaacagacc tgtagccgaa accgaactga tgtacaaaga cgctttcggt ttattggtag
     2881 cagtcattct atcggcacaa tgcacggata aacgcgtaaa tatgaccact ccgaagctgc
     2941 tgcaggacta tcccaccccc gaagccatgg ctgcttcaac tccggaggtg atttttgaat
     3001 acatcaggtc catctcctac cccaacaaca aatccaagca cctggtagga atggctcaga
     3061 agctgatttc agattttaac ggcattgtgc ccgacaatgt ggacgacctg caaaaactgc
     3121 ccggcgtagg ccgtaaaaca gccaatgtaa ttgcttcggt agtttacgac aaaccggcta
     3181 tggcggttga tacgcatgtt ttccgtgttt cggagcggct tggactgacc actgattcta
     3241 aaaacccgct tgaaaccgag cgtgaactgg taaaacacat tcctgccgaa attattccga
     3301 aagcccatca ctggcttatc ctgcacggga gatacgtgtg tattgcccgt aaaccgaaat
     3361 gcgaagaatg cggactgaaa gcatggtgca ggtattattc aaaaaatgca gcaaaaaccg
     3421 acaaataatt aaagaaaaaa gtgctgttca tttaataaat tccattaaat attcagtcag
     3481 gatttaaaac ctcccaaaag ttaaaaacag ttgctcattt tcaccgtatt tttttgtaat
     3541 tttgtagctt aaaattcaaa taaaaaagta tgaattcaaa tcttatgaac tccaccatgc
     3601 gtcatggtct gattcttgga atagtttttt ctgttaattt cattttatcc atttccggca
     3661 atatgttttt cggattgtta tcctacgtgg cagtcgggct catcatatat ttaacataca
     3721 aatatacaat aagttatcgc gatacagatt gtgccggaac aatcagttac cgtcatgcat
     3781 tgtcgtttgt tattctgctc tttgtgtttg cagcgctcat atcggcaatc gtcaaatatg
     3841 tttatttcca atacataaat accgattttc tgcctgaatt gatgaacaaa agcattgaat
     3901 tgatggagca aatcatgcct tcggtacctg aagaaacgta tgataatatg gaaaaaatga
     3961 tgtcgcccat caactattct ttgactgtaa cgtggatgaa tgtaattctt ggatttttag
     4021 tgggtttggt cattgccggt ctggtaaaaa aggaaaaaga cctttttgag aaatagaata
     4081 aaataagcaa aaatacatat aattaattga ttattaaaca tatacaaaat ggacatttca
     4141 gtagttgttc cgctgtataa cgaggaagaa tcgctcccca aactctacga atggatagaa
     4201 cgggtgatgg cagagaacga attcagttat gaggttattt ttattgacga cgggagcacc
     4261 gatggttcct ggagtatcat tgaggatatg caaaagttga atccggctaa gatacgcgga
     4321 atcaaattcc gccacaacta cggtaaatcg ccggctctat accggggatt cgatgctgct
     4381 aatggcgatg tggtgataac gatggatgcc gacctgcagg acagcccgga tgaaattcca
     4441 gaattgtatc acatgattat ttcaggcaat tacgacctgg tttcgggctg gaagaaaaaa
     4501 agatacgatt cgaaactcac caagaatctg ccttcaaaac tatacaatgc caccgcccga
     4561 agagtgacgg gactcagact gcacgatatg aactgcggcc tgaaagccta caggaatgat
     4621 gtggtgaaaa acatcgaggt tttcggtgat atgcaccggt atattcccta cctggcgaag
     4681 aatgccggct ttacacgtat cggcgaaaag gtggtaacgc accgaaaacg tgaattcggt
     4741 gaatcgaaat tcggcatgag ccgttttgtg aatggttatc tggatttgat gactctttgg
     4801 tttttatccc ggttcgggaa aaaaccgatg cattttttcg gcacgatggg cagcctgatg
     4861 tttatcctgg ggttcatagc cgttgtaata gtaggagcac ataagttata tgccctgagc
     4921 aaccatattg cggcgcctct ggtggttgat agtccgtatt tttacctgtc gctggtgctg
     4981 atgatcctgg gtacactgct ttttctggcc ggatttgtgg cggaaattgt tacccgcaat
     5041 tcgcccgaaa ggaataatta taaaattgaa aaagaaatat aatttttctg attaaaaaat
     5101 gaaacaattg ctgttggtcg gattcggcgg gtttttcgga agcatagccc gctactggat
     5161 gtcaaaatta aacctaacct gggattttca ttccattccg atgggtacgc tcgctgtcaa
     5221 tatcatcgga agttttctaa tcggttttat gttgggcatt tttatgaaca gcaacctgct
     5281 tcacactaat ctgaaactgt ttctggtagt gggattttgc ggaggtttta ccacattttc
     5341 gtcatttacc aacgagaatt ttctactttt gcaaaacggc cagtatctca cagcactcat
     5401 ttacattgga ggaagtatca taataggtat tttggctgtc tttgccggtt ttttaatagc
     5461 aaatcaactt tagaaatatg gaaacggaat atagtgtttt gaaaattttc atgagtaata
     5521 ccgataaatc ggggttggat ctgctctacg aaaaaattgt aaaagaagct caaaatcaag
     5581 gcatatcagg tgtcacggtg tttcgtggta ttatgggata cggtttgagc agtaccagaa
     5641 tagtgaattc aaaattctgg gaaatatctg aaaaactgcc tgtcataatc gaaatgacag
     5701 acaaaaccga aaaattaaac gatttttacc actcgatgga agaaaaactg aaatcaaccg
     5761 gcaaaggctg tctggtttat attcagccta tcaacattct attgcatcaa tccggaaaaa
     5821 agtaaatgaa tatggaaatc cttaaataca ccattccggc acttctggtg gtacttgtag
     5881 tgtacatttt gctctatcag ctcttcaaaa tggaacgaca ggcaagagaa accgaactga
     5941 aaaaaaccgc tctgccgctc atcacctcta cccgcttacg ggcttatgag cggctgacac
     6001 ttctgctcga gcgcataaat ccgaacaatc tgcttattaa cagcaccgca ccagatcaaa
     6061 cctgcctcga actgcagtcc gaactgctcg ccactatccg tcgtgaattt gaacacaaca
     6121 catcgcagca aatatacgtg agcgaattgc tctggcaaga aattgatgag gcacgtgaaa
     6181 atctgattca gctcatcaac acctgctcag cacaatgtaa agccgatgaa ccggcaacga
     6241 aacttgccaa catcattgta gaagtattca atactcccga agatacagct ctgaacgctg
     6301 cattgcgtat gctgaaagat gaagtaaaaa aactcttatg acgaaagtaa aatttaaaat
     6361 cccgatactg actatcctgt tgatttcggc attcattttt tcatgcgaaa aagatgaaac
     6421 ctgccgcaaa agccggattg tggaaacagg tgcaggattc tacctggact cggtgaatac
     6481 caccaccggc aaaagagtgc tcgtaaaatc taccattgac agtctgtggg tcagcggaat
     6541 tggtgttgat tcgttattgt ataaaaaaac aacaacaaat gccatcaagc tgccgttgaa
     6601 tcagtttgcc gaagaatcaa aattccggat gaaaacaaat aaggtagtag atacgataac
     6661 catacgctac aaaaacaaga ttgaatacct ctcgctcgaa tgtggaagca tacgtacgca
     6721 caccatcgac accgtattga cttccgggca ttatatacgc agtgtagcca ttattaatcc
     6781 aaccgttaac acactttcag ttgaaaacat acagttattt catactaagt agcatcctgt
     6841 tgttttgcgt tacggttcaa tccacagcac aaacaaaaga atccgtaaaa aaggatagca
     6901 ttccatttct tcagggaatt tggattgaag ccgatgtggc acctgtgttt gaacgggcac
     6961 tcattaacaa aaatgcttac agagcgcagg gaaatataca ggtgaatctg aaaaacaaat
     7021 attatcccgt aattgaattt ggctatgcgg gagccgataa aacggcatca aacggcattg
     7081 aattcagcac cagcggattt ttcggaaaag caggcgccga cttaagtatg ctgaaaccca
     7141 aacccaattc cactcagaaa aacaataatt ttctggttgg gcttcggctt ggagcgagcc
     7201 ggttcaacta ctcgttgtat aatcaaacca ttaccgataa ttactggggt aattcagaga
     7261 cattcaacct cgaatcaatc tcttcaacca aattgtggta tgaaattgca gccggtatgc
     7321 gggttgaagt tttcagaaat atctacatgg gctggaccat acgcaataag catttgattt
     7381 cgaaaaccga atcgggtcaa actactccct ggtacatccc cggatatgga atcgggaata
     7441 cgtccgtttg gggattgagt tacagcatag gttacagaat aaaataaacg acacaactac
     7501 atactaaatc attatctaaa aaaatttatg aacacaacac atgaactcaa caaagcagct
     7561 gacaatatac gcattttgag tgctgctatg gtagaaaaag ccaattccgg acatccggga
     7621 ggcgctatgg gtggagccga ttttatcaat gtactctatt ccgaatttct ggaatatgac
     7681 cctgctgatc cacattggga tgctcgcgac cgtttctttc tggacccggg acacatgtcg
     7741 cccatgctat attcggtact tgcactcacc ggaaaataca cgatggaaga tttgaagcaa
     7801 ttccgtcagt gggaaagcat tactccggga catcccgaac tcgatgtggc gcgaggtgtt
     7861 gaaaatacct ccggtccgct cgggcaggga catacctacg ctgtaggtgc tgccatagct
     7921 gccaaatttc tgaaagcccg ttttggcgaa gtaatgaatc atactatcta tacttacata
     7981 tctgatggag gcatccagga agaagtatcg cagggagccg gcagaatagc aggacacctg
     8041 ggtctggaca acctgattat gttttacgat tcaaacaacg tacagctatc tacctacacc
     8101 aattcggtaa caagcgaaga tgtagcaatg aaatacaaag cctggggatg gaaagtaatg
     8161 gaaattaacg gaaacgatgt aacagaaata agaacagctc tcaaagaagc caaaaaggaa
     8221 aaagaaaaac caacattgat aatcggaaac acgatcatgg ggcgtggtgc tgtgactgac
     8281 gaaggacaga gctacgaaaa catggtagat acacacggtc aaccacttgg agcaacaaaa
     8341 gccagttttg ccgccaccat ccgcaatctg ggcggcgaca gtgaaaatcc ttttgtgatt
     8401 tttgatgaaa cgaaagcatt gtatgccaaa cgtgaagccc aactcagaga aattgccgaa
     8461 gaaaagaaag ctgcccgaca agtatgggct gacgaaaatc ccgaaatggc tgccagactc
     8521 aatcagttct tctccggcaa actgcccgag ctggactgga aatcaataga acaaaaaccg
     8581 aatcaggcta caagagccgc ttctgctact gtactcacat tcctggcgga aaacgttgaa
     8641 aacatgattg tttcgagtgc cgacctgagc aattctgaca aaactgacgg ctttctcaag
     8701 aaaaccaaag ccctgcagaa aaatgatttt tccggaaaat tccttcaggc cggtgtggca
     8761 gaactcacca tggcctgttt gtgcaatgga attgctctac acggcggtgt gattgcagca
     8821 tgcggtacgt tcttcgtatt ctccgactac atgaaaccgg cagtacgtat ggctgcactg
     8881 atgcaactgc ccgtgaagta catctggacg cacgatgcat tccgcgtggg cgaagacgga
     8941 cctactcatg agccggtgga acaggaagca caaatccggc tgatggagaa actgaaaaat
     9001 catcacggaa aaaactccgt tttggtactc cgtccggctg acgtggaaga aactaccgtt
     9061 tcgtggaaaa tggcactgga aaatacggat acacccactg ctcttatact atcgaggcag
     9121 aacattacca accttccggc caaagaccgc aaaacggctg cctacgaggc cgaaaaaggg
     9181 gcttatgtgg tggaatgcga cggaaatccg gatgtggtat tgctggcatc gggctccgaa
     9241 gtagctacac tggcagaagg tgctgcgctg cttcggaatg atggaatgaa agtaagaatt
     9301 gtttcggttc catccgaagg tttgttccga agccaacccc aagcctatca ggaaagtata
     9361 ttgccaaaag acattaaaag atttggactt acagccggcc tgccggtcaa tctggaaggc
     9421 cttgtgggtg aaaatggaaa agtgttcgga ctggagtctt tcggtttttc agcttcttac
     9481 aaagtgcttg acgaaaaact cggattcact gccgaaaacg tatacaagca agtgaaagct
     9541 cttttctaaa aaagtacgaa ttggttttga gcataaaaga actattcaac tcttcagcgt
     9601 ctgatttttt caatccctgg agagttgaat tgtttttatt ttcaagaaca aaaagctgtc
     9661 tgataaatat ccttcgggaa acaggtgtgc tcgctgacta acagtcttat tttgaattaa
     9721 ttaacttttt tctaaatgga ttttatcacc attcttctca tagctgtcgg cttatcgatg
     9781 gatagttttg ccgtttcgat aagccgggga atgtgcactc aaaaacttaa attttctcag
     9841 gcatttttac tggcagcaat ttttgctgtt tttcaggggc taatgccatt gattggattc
     9901 ggtgccggac aggtttttgc tgatcagatt cgggctatcg accattggct ggcatttgca
     9961 atccttcttc tcatcgggat aaaaatgatt tttgagagtg tgaaaccaga taaagtaaat
    10021 gaaacaaaga gaaattgtaa caacgaatgt aactgcgaca ataaaaactt cagtctgaaa
    10081 tatcttgtaa ttcttgctgt ggcaaccagc atcgatgcac ttgctaccgg cttgatattc
    10141 acaccttatc cggataggat actgatggct gtttctttaa taggcatcac aacttttgta
    10201 ttttcatacg caggcgtcaa tcttgggcat aaattcaggc acggattcaa atttaatttc
    10261 gaaacagcgg gaggtatagt attgattatc ataggactaa agatactgat tgaacatctg
    10321 aatgcataaa aaaatgccgg atcaatcagt ttgatttccg gcattttgtt tttcgaaatg
    10381 atttttatct ttttccttac tttagtttgg atttaatcca ttcatccatt gctttggcag
    10441 cccgacgtcc gtcgcccata gcaagtatta cggtagctcc gccccgcaca atatcgcctc
    10501 cggcaaaaat cactggcatc gatacggatt gcaaagtatc ttcatttacc accaaagtac
    10561 cccttttact ggtctgcaat tcgggtgtgg actgagccag cagcggattt ggtgataccc
    10621 caacactcac aataacctca tccacctcta ccgttacggt tttgccggga acttctaccg
    10681 gcgaacgacg tccggattca tccggttcgc ccagctgcat cacctgcaac accatgtgag
    10741 taacgcgtcc gctttcgttg ccaatatatt ctatgggatt atgcaacgtc atgaagtcga
    10801 tgccttcgct tttagcgtga attacttctt cgcggcgggc cggcatttct tcttcggagc
    10861 gacgatatac gatgatggct ttttcggcac caagtcggcg agccgttcga accgaatcca
    10921 tagcggtatt gcctccgcca attacagcca cttttttacc tttgtaaacc ggtgtgtccg
    10981 aatccggatt ggcagcatcc atcaggttca cacgtgtcag atattcgttt gacgacatca
    11041 caccaaccag gttctcgcct ttgatattca taaaacgggg caagcctgca ccgctggcta
    11101 caaaaattcc ctgaaatcca tcctgctcaa gttcgtgaat agcaatcgtt tttccgatga
    11161 tgcaattgtt catgaaacga actcccatct gtcgtaaatt gtcaatctcc acatcaacaa
    11221 tatgatttgg caacctaaac tcggggatac cgtatttcaa tacaccgcct atctcatgca
    11281 atgcttcaaa tacatgcact tcatatccca gttttgccat atctcctgcg aatgaaagcc
    11341 ctgccggacc ggagcctacc actgccactt tcatcccgtt ggcaggtgct gtttcaggca
    11401 cagaaatctg tccgctttca cgctcgtagt cagctgcaaa gcgttccaga tgacctattg
    11461 caaccgattc tttattcatt ttcacatgaa tgcattgtgc ttcgcactga cgttcctgcg
    11521 ggcacacacg tccgcaaact gccggaagtg ccgaagtttc tttcagtact tttgctgctt
    11581 ccaggaattc accccgttcg atatttttga taaaatccgg gatattgata ttcaccggac
    11641 agccatccat gcaggtggga ttagcacaat ccagacaacg gtgtgcctcc tggatagcca
    11701 tctgaggagt taaacccaga ttgacttctt cgttccgaat atgacttctg tattcggaat
    11761 ccagctcagg catttcaaca cgatgtagat tcgttctttc cttcgctttc atttgagcac
    11821 gcaattcaac gcgccaggta tcatctcttt ttgtcatttt ttatgtaata cttttctttc
    11881 catttcttct ttttcctgtt ctctgtaacc tcccaggcgc atcagcattt catcaaagtc
    11941 aacctgatga gcatcaaatt cgggtccgtc cacacataca aatctggttt tccctcctat
    12001 tgtaacacgg catgcaccac acattcctgt gccatctacc atgatggtat tgagtgatgc
    12061 taccgtgggt acgttgtatt ttcgggtgag ttcgcttaca aacttcatca tgattgccgg
    12121 tccgatggtg acacacatat ccactttttc gctctgaatg acagactcga caccatttgt
    12181 aaccaaccct ttgtttccgt aggagccatc gtcggtcatg attatcactt catcggaatt
    12241 ttcacgcatt tgttcttcca ggataattaa atcttttgtc cgtgcagcca gtacggtaat
    12301 taccctgtta ccggcttttt tcaatgctgc cacaataggc atcatgggcg ctgtacctac
    12361 tccaccgcat gcacaaacta ccgttccgaa attttcaatg tgtgtagctt ttccgagcgg
    12421 cccgaccata tctgtaatgt aatctccggc ttcgagagca catattttgg ttgaagaaac
    12481 acccgttcgt tgtattacaa gtgttattgt acctctttca acgtcagcat cggcaatggt
    12541 tagcggaatt cgttcgccct tctcacctac ccttaccatc acaaaatgtc cggcccggcg
    12601 tgctttagca atcaacggag cttctacaac gaatttcaca acattggctg agaaattttc
    12661 tttactaatt attttattca ttcttttttt aaaatcaact tttggcgggt tttcaattct
    12721 gtttttttac ggagtttccg gatctgtctg aatgggttcg gtttcctcgt tgcttatttt
    12781 cagatcggga atcggcaacg cttcaatcgg aactctctcg aaaatgctgt cgttcgattc
    12841 accatcgtaa gcagccgaat aatcgctttc gcatttataa tctcttgaaa ttttttcttt
    12901 tggttcggaa ggaaattttg ctctgtactt tgagaatttc tggtcggcaa gcactttttc
    12961 cataaataag gcaaataccg gcaatgcagt tctgcttccc tgtcccatgc ttccgcttcg
    13021 gaagtgaatg ctacggtgtt cggctcctac ccacgaaccg gcaaccagtt tgggtgtaac
    13081 tccgatatac caggcatcgg agtagttgga cgaagtacct gtttttcctc cgaattcggt
    13141 atcccagcgc atcaaatcgt agttccacaa ggctctggat gttcctccgg gttcggtgat
    13201 accggctttg agcaattcgg tcatcagaaa ggcattttcg tacgatatta cccgcttggg
    13261 tctggctttg tgttcgtaaa tcacatatcc gtccttatct acaatttttg taacaattac
    13321 aggtgccata tacatacctt catgagtcac cgtactgtat gaattgacca tttcgagcaa
    13381 cgacacatcg gaagctccca gacttagcgt gggtacttct tccagtttgg atttgatgcc
    13441 gagcagacgc gctgcaccgg caacattctg aatgccaaca tctttggcaa gttgagcagc
    13501 tactgtgttt actgaacggg caaatgccct tttaagggtc agattgttat aggaataact
    13561 cctgttggca ttttttggtt gccaggcata ttttcctttt ccccaagtaa atggtttatc
    13621 ttggtaacgg tcacacggac tttttccggc tatcattccg gctgtatata caaaaagctt
    13681 gaatgtggaa cccggctgcc gcttgctttg agccacttta tcgtagtgcc agtaattgta
    13741 atcaatatcg cccacccagg cttttacctg cttggtatca ggctccatgg ctacaaaacc
    13801 acaatgcaga aaatggttca ttaaccgaat ggagtccatc gagctcatag taacttcttt
    13861 agtgccttct ttatagtcaa atattttcat tttacggggt tgattcagat atttctgaat
    13921 ggaatcttca tcaccgttaa attgcacgct caacgatttg taaaccgatg atcgcttagt
    13981 aatttcggga ataaaattgg taagttcctt accgttttca tcacgccagg gattctgacc
    14041 ggaccagtga tcgaaaaaat ttctctgcag agttttcatt tgtttatgaa cagcttcttc
    14101 ggcgtattcc tgcatttttg agtcaatggt tacatatatt ttgagtccgt cggcatagat
    14161 atcgtaacca tttgtttcac cccatcgttc gagaaacttg gtgaggaatg aacgaaaata
    14221 gggtgctact ccttcgttgg gtttttcgac tttataatta agttttatgg gtaattgttt
    14281 cagcgaatcg gcttcgttac tgctgatgta attattttgg gctagctgtc cgataactac
    14341 atttctccgt tgtttggacc gatccggatt gaggtaagga ttgtatgctg tgattgcttt
    14401 cagaatgcca acaagcacag ccgattgttc ataattgagt ttatcgggcg tagtgttgaa
    14461 ataagtgcct gcagcagtat gaattccgta tgcattgctc ccgaaactaa ctgtattgag
    14521 atacatttgc agaatttcat tcttagaata taacaattca agtttcagcg ctgccgacca
    14581 ttcctttgtt ttttcgatag gaagtcgcaa gccgggcaga gagccaagta agccgcgtga
    14641 atactggctg cgggttttga acatattttt aaccaactgc tgagtaatgg tacttgcacc
    14701 gcgtggattt ccgcgcagca tatctcttgc tgcagaggcg agtcctcgaa aatctattcc
    14761 gaaatgctta tagaaacgtt tgtcttctgt gctgattagc gtatttatca tttcatccga
    14821 aatctctgac agagtaacct ctttgcggtt ttcggtgaaa tatttcccga taagttttcc
    14881 gtcggaactg attatttctg atgaataaga ttgaggagga tttgcaatgg ctttgatact
    14941 gggcgattta ccatacagat acaaaaaatt aatatcgacc aaaagcaaat aaaatgcaaa
    15001 aagaaataca attgtaagaa atgtattcaa tacttttcgc catgtgggga gattcttgaa
    15061 cctcagaata cgcctttcgc gccattcaat aaaaagaaat aaccagtttt tcagaacatt
    15121 gaatattctt ttgataaatc ttttaatttc tgtgaactcc atgttatatt tttttgattt
    15181 cagtacaaaa atagtagaaa tgtttcaaac tttgagctta aggagaaaaa atgtaagctt
    15241 tttgaccgaa ttcaaaacaa taacatgaac tcgtattgtt tttttacaat gcgacatcta
    15301 cggggctacc cattcaggaa cggaagaacc gtatgataat agaaaattaa tttttattat
    15361 gaacagaagc accgaagaaa ctacattata gctctattaa cagaagtcag gactgtctcc
    15421 ttattgttct caaaatctta tgaggaaaga aacgtaaaat aaaaaaaggt cttctgtacc
    15481 gaaaaccttt tttttatcat tctaaagacc gaattctttt tttatttcat cgaccatatc
    15541 gagtttttcc catgtaaata actccacatt tttcttaaat tctttgcccg atgcatcgat
    15601 aaatgttttt tccacttttt taggagtacg tcctacatga ccgtatgaag ctgtttcttc
    15661 gtatatcgga tttctgagtt tgagtctttt ttcaattgct ttggggcgca tatcaaacaa
    15721 tacagctact ttttcagcta tctgcacatc cgtaagattt acgttggatt ttccatacgt
    15781 attcacataa atattcatcg gattggctaa accaatagcg taagccacct gaaccagcac
    15841 ttcgtcggaa actcctgctg ctacgaggtt tttggcaata tgacgtgccg cataagctgc
    15901 cgaacggtca acctttgagg gatctttacc cgaaaatgca ccacctccgt gagctccttt
    15961 accgccgtaa gtatccacaa taatttttcg gccggtaagt ccggtatcgc cgtgaggacc
    16021 accgattacg aattttccgg ttggattgac atgcagtaca tagtcatctt ttatcagcct
    16081 ggcaaattga ggaacctgag ctttcacacg cggaatcagg atattgcgca catcttcctt
    16141 aatgatggaa agcatctcat tatcagctct ttcctgccct ctttgatcgc tgggatgaat
    16201 aaaatcatcg tgctgagttg atatcacaat ggtatgcacc cgaagcggct tgttattttc
    16261 gtcgtattct atcgttacct gcgatttgga gtcgggacga aggtaagtca tcagcttgcc
    16321 ttcgtttcgg atcaccttca gttccttgac caatgcatgc gacaaatcca gtgatacagg
    16381 caggtaatta acagtttcgg tacaggcata accaaacatc ataccctggt cgccggcacc
    16441 ctgattcatc ggttcttcgc gttcaacacc ccggtttatg tccttggatt gctcgtgtaa
    16501 agcagagaaa ataccgcatg aatttccgtc aaacatatat tcactcttgt tgtaaccaat
    16561 acggttgatt acctttcggg caatggtttg aatatcaaca taagcctgtg agcgaacttc
    16621 acctgctact accacctgtc cggtagtaac cagcgtttcg caggcaactt tcgagttggc
    16681 atcctgagcc agaaaattat caagaatggc atctgaaatt tgatcggcca ctttgtccgg
    16741 gtgcccttct gacaccgatt cggaagaaaa taaataaccc ataatattga tttttgtttt
    16801 tcgccggcag gatgtataat tacagcaact tactagcaat catcacattg ccggcagatt
    16861 aataaatatt tattgggaaa gaaaaaatgg actgtaaaag aaaaaaacgg aaagttagtt
    16921 acaatttagc attttttctg tggttgcaag cagtccaaat ctatccacat ttgtttcggg
    16981 tgcaaagata caaaaaatac cgaaaaaggc caaaaaacaa aaatattttt ttagctaaaa
    17041 aaaaatcatt tcagcaattc ctgaatatct gcttcgatat cggccggttt cactctcgat
    17101 tcatatcttt tcaacgtttt tccgtctttt cctatcagaa atttggtgaa attccatttt
    17161 attttctccg gttcggcatt tggaacgctg tttttcagat aggtgtaaac aggatgagca
    17221 ttttcaccgt tcacatcaat tttctcaaac atgggaaatg tgactccgta gttagcctta
    17281 caaaattcaa gaatttcttc gttacttccg ggatcctgat tggcaaactg attgctgggg
    17341 aaaccgagaa ctacaaaacc ccggctgctg tattttttat ataattcttc gaggcctgca
    17401 aactgtggtg taaatccgca cttactggct gtatttacca cgagtacaat tttacctttg
    17461 taatcactca gtttaatttc ttttccctgc atattcaggg ccgaaaaatc ataaaaagtt
    17521 tgtgttgtgg agtttagagc aacctggttc gcattttttt cagattcacc gtttttctta
    17581 accgatgaac aacctgctat agctaaagcc attataacta tgatgatcaa atttttcatg
    17641 atgattgaaa ttttaatttt tacataaaac aaaaatcatt tcgaatggtt cagccaattt
    17701 tcaatatatt cttcacatta tcgaaaactt taataacttt acaaaaataa tatgaaaaga
    17761 ttatgttagg agcaattatt ggcgatatag caggatcacg ctttgaattt aacggattca
    17821 aaagcacccg ttttgagttg tttgatcctg cctgtgatta caccgacgat tcgatttgta
    17881 cggtggcaat cgctgacgct gttttgaaca aaaaatcgta tcaggattca ttgctggaat
    17941 ggtgtagaaa atatccaaat ccgatgggaa gctacggcaa tcggttttca gcctggataa
    18001 aaagcgaaaa cccacaacct tatggcagct tcggcaacgg atctgccatg cgtgtaagtg
    18061 ccataggctg gctttttgag gatgaagttg agacactcag acaggcagca ctttcggcca
    18121 tccccaccca caaccatccg gaagggataa aaggtgctca ggctgtggca ttggccattt
    18181 tcagaagcag aaacggagct gataaatctg aaataataaa agaaacggag cagaaatttg
    18241 gttacaatct ccaactcacc tgcgatgaaa ttcgtcctgc atactgttac agtgagacct
    18301 gccaggacac tgttccacaa gccattacag catttcttga aagtacatgt tttgaggatg
    18361 ccgttcgtct ggccgtttcg ctgggcggcg atgccgatac cttaacagcc attaccggga
    18421 gcatagccga agctttttat aaggaaattc caagtgtaat tagcaaaaaa gcactggaat
    18481 atttaccaaa agacatgaaa tctgtcataa atcaatttta cagaagcatt taaaatattt
    18541 ggaaatgcat ttcaagaggg gcaaaacttt tttatccgtt tgattatttc ataaaaagag
    18601 ttaactttgc aaacagaaat ctgaacaaat tcagttttca aacattatac atataaatgc
    18661 tggggtgcct taatatttcg ggctgagatc ataccctttg aacctgcacg ggtaattccg
    18721 cgaagggaat aagaatcaat caattcacat ttcaaccgtt ctaactggct ctctcacacc
    18781 attttataaa tcataaaata aaatcagatg agaagacact ttctttttac actcgtgttg
    18841 attagtacac tggctgttca gtctcagaat ttcattcggg gcaaagtgct ggatgataac
    18901 aatcagccca tccggggagc ctacgtaata ctcaaacaaa catctcgaac agtaatatcc
    18961 gatccgaaag gtgaatttta cttcggaaat ttaagcgact tcgcctatac gcttcaagta
    19021 aaatgcattg gatatgagga ttttcagaaa aatgttcaga caggttcaga tattcaaatt
    19081 gtactgacgc acatttcgat aaatcttgaa gaggttaaca ttctggccac ccgcgctacc
    19141 gaccgttcgc cggtggcatt ttccaatgtc acgaaacaga aaatcgaaga gcgaaattcg
    19201 ggtcaggatt tgccttatct gctggcattt acaccttcat acatcaccac ttcggacgcc
    19261 ggcaccggga ttggatatac aggatttcgc attcgtggtt cagacgctaa ccgtacaaat
    19321 gtcaccgtaa acggaattcc gctgaacgac tccgaatcac acagtgtttt ctttgtaaac
    19381 atgcccgatt ttgcctcatc gctccattcc gtgcaggtac aacggggtgt aggtacttca
    19441 tccaacggtc cggccgcttt cggtgcgagt atcaacatgc agaccgaagt gttgaacaat
    19501 aagccttatg ccgaagcagg aattactgta gggtcattta acacgctgaa aaacagcgtg
    19561 atggcaggca ccggaatgat aaataatttt gcggtcgatg tacgcctttc gaatattatt
    19621 tctgatggtt atatcgaccg cgcgtcggta gatatgaaat cgtatttcat ttcgggcgga
    19681 tactacggcg aaaatacact ggtaaaattc attacttttg gcggaaaaga aaaaacctat
    19741 caggcatgga acggggtgga tctggaaaat cacacacgta cttacaacga actcggcatg
    19801 tacaccgaca acaaaaacga aacaaaattc tatgataatc agacagataa ctacaaccaa
    19861 acacattatc aactgcattt cacacaggtg ataaatgccg gcatgaactt aaatgcctca
    19921 ttacactata cacggggtaa aggatattat gaagattata aaaccgaccg gaaatacgtg
    19981 gagtacggct taactcctgc tacagtaaac ggaagcatac tcaggaaaac cgatctgatt
    20041 cgtcagaaat ggctcgataa tcattttgga ggtgccacag tttcattgaa ccggaaatac
    20101 gaaaaactgt cgttgacggc aggtggagct gccaacaaat acttcggcga tcattacggc
    20161 aaagtaattt gggtacgata tccaaacaat gcagcccccg gccacacata ttacttcaac
    20221 gaatcaacga aaaccgatgc aaatatttat gcaaaactga attacacgat taccgacaga
    20281 ttgcatgcct atgctgactt tcagtacaga ttcattgaac ataagatgag cggagaagat
    20341 gataaatacg atgcggataa aggttccatg cgggatatca cacagtcgca tatatttcat
    20401 tttttcaatc cgaaaggagg cctgaattat cagattaaca aatccggcag cctttatgct
    20461 tcgtttgcga tagccaaccg tgagccaaac cgcaacaatt ataccgatgc cggaccgaat
    20521 gaggttcccg aaagtgaacg attgtatgat accgaattgg gatacagata tttatccaac
    20581 aatttaagtc tgggagccaa cctgtattat atgaaataca aagggcagct tattcttacc
    20641 ggaaaaataa gtgatattgg cgaaatgctt acttcgaaca tacccgacag ctaccgggca
    20701 ggtgtggaac tgatggccgg attgaagatt accgaaggtt tacgctggga tggaaacctg
    20761 aatctgagca gcaacaagat caaaaatttc agagaatacg ccgacgagta cgatgctgaa
    20821 tggaaccgaa ccggaacaaa agagaatttt tttaaaacaa caaacatatc gttttcgcca
    20881 aactctgtgg ctaacagtat tttaagttac tcattcaaag gtatcgaaac agcattttat
    20941 tccacattcg tcgggaggca gtacctggat aacacatctg acgtgaaccg ttcgattgat
    21001 gcttactttg tgagtaacct taggctgggg tactcgtgga aaatgaaaaa aatcaaatca
    21061 gttgatttta atctgctcat taacaacctt ttcgataccg actacgaaac aaacgggtat
    21121 aactggtaca cctattacct ggcgggtgaa agaataaacg aaaaaagata tttcccgcag
    21181 gcaggcattc actttttatt ttctactgca gtgaaattat aataccgttg atattctatc
    21241 ttttgaatca aagaaaaacg tcggaaaaat taatctcaga cgttttttta ttgaattatt
    21301 ttttgtcgtg tttgcttttg gttttttgat atttgaatcg ctttattttt tgcgaagcgg
    21361 tcttctcaaa aggttcttcc atgatgtcca cccggttaat ctgagaagag cgtcccaatt
    21421 ttgaattaat atcttttttt agttggtttt tccaatcgtg gtaatgctct ttcttttctt
    21481 cgatgattaa tttgatgtgt tcaatgtttt tctctgccat ttccagatcc agcaaaattt
    21541 tagccaccag gaaaccatct tcttcaacaa ccagtgattc agacacataa gctgtattat
    21601 tgataattga ttcgatatcc tcgggataaa tattttcgcc gctcgctcct attatggtat
    21661 ttttcagccg acctttgata aagagtcttt ttttcttgtc gaactgccct aaatcaccgg
    21721 tacgaaacca cttgtcggtg gtaagcactt cggcggtagc ttccggattt ttataatatt
    21781 cgagcatcac attcggtcct ttagcccaga tttcacccac accaagttca tccggttcat
    21841 ttatcctgag ttccacaccc tccaaagcat atcccgtaga ttgcagtttg gtttgatgag
    21901 gagccgcacc tgccagcagc ggggctgttt cggtgagtcc atacccgata gcataaggaa
    21961 attttgcctc cttcagaaat cgttccacat aataatcgag tttagcaccc ccaataccaa
    22021 agaatttcaa cctgcctccg aaagttttat acaatttctt tccggcaatg tggtgaatta
    22081 tttttcgggt tacgggagtt ctgtaaacca actttttcaa catcgaagtt ctgaacttga
    22141 gataaatctg atttttatac agtttttcca ttatcagcgg caccgaaagc atcatggttg
    22201 gtttaatttt ttccatagct gctattaaaa caggcgcagt gggcagtttt tcgagaaaat
    22261 aaatggtcgc accgtacata atgggataca acatcccgat ggaattctca taagtgtgag
    22321 acagtggtaa aaatgaaaga aatacgtcgt tgtgatttat tggctgaact gtataggatt
    22381 gcatggccac aaaagtaaca cttttgtgct tcaataccac tccttttgat cgtccggtag
    22441 ttccggatgt atagattata gctgctacat catcttctgt tacatccgtt tcagttattt
    22501 tatttgaatc aaaagttaat tttgaaatgt cactcaagaa agtaaaatca tccagtttaa
    22561 tttttactat cagatccgga gtttcaattt gacttacttt tggaaataag cgttcactca
    22621 cgattaaaac ttttgactct gaatgagtta acacattttc aacctcttcg gttgtaaaat
    22681 caggtaaaac aggtacaata atcaatcccc gggatactac cgaaaaataa gcaatcaccc
    22741 agttgggcat gttatgtgaa aaaagggcaa ctttatcccc ttttttcagt ccggatgaaa
    22801 ataagagagc tgcaaattca tcaatttttt cacctaattc cttgtaagta atgggtttgc
    22861 cgtctacaaa cgaaacagaa gtacaatcgg caaattgact gacagaataa tcgatcagcg
    22921 atttgagggt gagaggagtt ggaactattt ttttatgcat aaatgatatt aataagactc
    22981 tgattaaatg aaaaatgtgc gcaaagataa tgaaataatc ataacttatt gcacattttc
    23041 agttcaattg tgcatttttt atttatatat taaaaatata cagggttttt tgttcagatc
    23101 cggtttttgt gatttccagg ccgaaactgt ttttgtatgt ataaattctg tctcaagggt
    23161 aatatctgcg gctatacaga gtttcgtagc cggattacaa gcagtcagaa tgtcgtccag
    23221 cattttcatg tttcggtaag gagtttcgat aaaaatctgc gtttgatttt cggagtaaat
    23281 tctgctttcc atctttttca aagcttttat tctttccgat tgctgtacag gcagatagcc
    23341 caggaaggca aaactttgtc cgttgaaacc cgaggccatg agcgatagca aaatggacga
    23401 aggacccacc aaaggcacta cgcgaaagtt ttgctgctgt gccagcgcca ccacatcggc
    23461 tcccggatcg gcaatggccg ggcagcctgc ttcggaaata acacccatgt cgtttccggt
    23521 tttgagtggc tgcagaaact gagctatttc ttccggttta gtatgctcat tgagttcgaa
    23581 gaaagtgagt tcatcaataa taatatccct gttgattttt ttcaggaaac gacgtgcagt
    23641 gcgtatattt tcaacgataa aatagcgaat ggatgtaata atatccgcat ttccccgtgg
    23701 aagtatttta tccgtttcgg tatcaccgag ggtagtggga attaagtaaa gcccccccgc
    23761 tccccgaagt ggggatatgt gtgaatcctg tattttcgaa ttgttattca tggtttatcc
    23821 taattgtaaa attatgagta taatcagaat attcaaaaaa atcattgttc agaagttttc
    23881 aatttttcca gttcttcggc cagaatctcc cattcgtaca ttttctgttc cagttcccgt
    23941 tgctttttct gcaaaagcat aataaattca ggatcagatg ctttagccgg actctccagt
    24001 tgtgtcagca tttctgaaat ttccttctca agtttttcca ctgcattttc ggcagtttcc
    24061 acgtcttttt cggcttttcg gatttttttg ctcagttctt ttcgggcttc ataagcctcc
    24121 ttacctcctg aaggggaaat aattcccgta ttttcatcat tttcgggatt atttactttc
    24181 gtattcttca aaaatccccc ttgtgacgaa tttaggggac ttcctttaag gggttgtgtg
    24241 acaggacttc caagttgatt caaatgctca atattctttt tccgaaggaa atcatagatg
    24301 cctcccaggt gttcgcgcac ttttttgtcg gcaaactcat acactttggt aaccaatccg
    24361 tcgagaaatt cacggtcgtg cgaaacgatg atggcagttc cgtcaaaagc tctgatggcg
    24421 tctttcagca catctttgga tggcatatcg aggtgattgg tgggttcatc aagaataagc
    24481 aggttaaccg gttcgagcag caggcggatc atggcaaggc ggcttctttc gcctcctgac
    24541 agcactttca ctttcttgtc ggatgcttcg ccgccaaaca taaacgctcc cagaatatca
    24601 cggattcggg tacggatatc tccggttgcc acgtaatcaa tggtttgaaa aactgtgaga
    24661 ttttcatcca gcagtgatgc ctgattctga gcaaaatagc cgattttcac attgtgtccg
    24721 agtttcatgg ttccggaata accaatttca tccatgatac atttcaccag cgtactttta
    24781 ccttcgccat ttttacccac gaacgatact ttttctccgc ggttgataat cattccgatt
    24841 ttatcgagta caaggtgatt gccgtaagcc ttcgagagat tttcaatttc aaccggaatg
    24901 gagcccgaac ggggtgaagg aggaaattta aggctcaacc tggaagtgtc ttccagatcg
    24961 acttcgatgc gaacgagttt ctccagctgt ttgatacgcg actgaacctg aacagatttt
    25021 gtagccttat accggaaacg ttcgataaaa tcttcggtat cctgaatcat tttctgctga
    25081 ttttcatacg ctcgcacctg ttgttcgtgt ctttcttttc tgagttcgac gaatttggag
    25141 tagttcacgt tgtaatcgta aattctacca agtgaaattt cgatggtacg gtttgtaact
    25201 gcgtcaataa atgccctgtc gtgtgaaacg agcaatacgg cgcttgctga attgaccaga
    25261 aaattctcca accattgtat agattcaata tcgaggtggt tggtaggttc atcgagcagc
    25321 aggatgtccg ggtcctgaag cagaatttta gccaactcga tacgcatacg ccatcctccg
    25381 ctgaattcag agcactgcct gtcaaaatct ttccggagaa agccgagtcc catcagtgtt
    25441 ttttcaattt cagcatgaaa atttccggga ttgatgattc ggtaatgttc gctggctcgg
    25501 ctgtgttttt cgataattct gaggtattca tcgctatcgt agtcagtccg gctggccagt
    25561 tcaccggcat atttttcaat atcctgttgg acctgagtaa tatgttcaaa cgctttttcg
    25621 gcttcctgaa gaacgcttgt gccttcatta tgcaccatat tctgaggcaa atagccaatg
    25681 gtaaggtccg gggaaattac aatatttccg cgagttgggt tttgttttcc ggcaatgagt
    25741 tttagaaggg ttgtttttcc tgcaccgttt ttacctacaa gggcaatttt atcttttgga
    25801 ttgataagaa aaccaatgtt gtcaaataat ggcgatccgc caaactctac agtaacctgc
    25861 tcaatggaaa tcatatcaat atggtgaaaa tctttactta ttagagagat ttgtattttt
    25921 cttattttta tcgaccagag tccagaggat tttggtaagc aggctgcaga ttatcaaaac
    25981 cactaaagca agtacgccat tggaaagatt tcttcctaac acagcaagaa ttataaatac
    26041 tgcagaaatg cccataagta taaatgtaac ctgcctgtgc ttcaatccca ggctcaacat
    26101 caaatgatgt acgtgattac ggtcggctct gaagggcgag taaccttttt ttattctggt
    26161 aatcattacc cttatggtat caaaaagcgg cacaatgagt accgtaaaag ccacagcagg
    26221 tgcggcagat atctggtact tttcaggcaa atctccggag gcgtttattt cacaaaatga
    26281 aaaaataaac acattgaaaa tgtatccaag taaaagcgaa ccggaatcgc ccataaaaat
    26341 tttattctta tcagaaaaca cattgtagta aaagaacaca atcaaagagc cggtcattgc
    26401 aaaagcggtg atagataaat cgggatgacc gatgagctgg aaatacacag caaagaaaag
    26461 cgagtataag attccaagtc ccgaagccaa accgtcaatt ccatcaatca gattcagggc
    26521 attgacaatg acaatgaata cgaaaaatga caaaaaatag ctgaagatca gcggaatttc
    26581 ataaataccc agaaaaccat gcagatttga aagtctgata tcggctatta caatgagaaa
    26641 aaatgcggcc aacagttcac cgaaaagctt tgaagatact ttgatattga ttaaatcatc
    26701 gataaaaccc acaatcagaa taataaatag tcctaaaagt gtaaactgaa ccgaataaaa
    26761 cacctcaaaa gaaaacagaa aaacagtgat cagaaatgat acaaagatat taacaccccc
    26821 tacattcgga atttcccccg agtgagaagt ccttttattc ggacttacca caaaattgta
    26881 gttcttagct atccggatca ctacgggcat aaaaaaatag ccgatagcaa atgataccag
    26941 aacaaccaga aaaggaaatt tgtgtatata aggaatgatt ttatccatca gttttgcatt
    27001 ttttcaatat cgagtttctc gtactgagaa ttgttgctga caaactcggg tacaatcgat
    27061 ttcatctgcg atacggtaag gtagttttta ccccgatagc tgtagtttat caattcatca
    27121 attttaactg aaacttcatc gtaatcatac tcacgcacag ttgccaccat tattttcgga
    27181 tggtgagtct ctttggtaat ctctttttta ttcaggagtt cttcatacag tttttcaccg
    27241 ggtcgtaatc cggtgaaaac aatcgggata tcaacttcgg gtttaaatcc ggccagcata
    27301 atcatctttt tagccagatt caggatcttc accggttcgc ccatatcaaa tataaatatt
    27361 tcgccaccat tgcctgctgt tccggcttcg agcaccagaa ggcatgcttc aggaattgtc
    27421 ataaaatagc ggataatctc gggatgtgta accgtaacgg gacctccgtt ttcgatctga
    27481 cgcttgaaat aaggtattac agaaccgttg gaacccagca cgttgccaaa tcgtgtagtg
    27541 ataaatttgg tagtgttttc attttccgaa aacatttttt tatttagcga ctgaacgtaa
    27601 atctcagcaa ttctttttga cgctcccatt acgttggtgg gattcacggc tttatcagtc
    27661 gataccatta caaacctgga aacattgtat tttactgcca gatcagccat attttttgtt
    27721 cccattacat ttgtctgaat acattcagtg ggattatctt ccattaaagg tacatgttta
    27781 taggctgcag catgaaaaac aatgtccggc cggtaagtat ccatcatcag tttcattcgt
    27841 tcgttgttcc tcacatctcc gagaaaagcg ctgaaattct gattcggata tttttctttc
    27901 aatttcagaa taatttcgtg cataggcgtt tcggcctgat ccattactac gataagtttt
    27961 ggactgaaag caatgctttg tttcacaatt tcgctgccaa ttgagccagc agcaccggtg
    28021 atgagcacca ctttgttttt taattctttc gaaatacttt ctgttgaaat tttgatttga
    28081 ggcctttcga gcaaatcttc gatctgaatc gatttaatct gacgcggtga aagtgtttcg
    28141 ccttcccaaa cactcataga ggggacagta agaaccgcca gcccatggtt aataaatata
    28201 tccaggtcag tttgaggatt aatttcttcc attttcagcg gtgatacaat cacagcacgc
    28261 gcttttctga ctattttctt taccgattct tcagtgagtt catacacttt cacacccatc
    28321 aacatttttg aagtcacttc cttgtctgta tcaataaatc ccacgagctt gtatttgatt
    28381 ctttcgttgc ttctgagcat tttagctatt gaggttccgg ccgattttgt cccaaatatc
    28441 attaccggag tagtattgcc taggtttgat gaaatagtat catgtactgt tttcacaatc
    28501 aaacgcatca aaaataaaag gaaaaaagcg gtaacagcgt aaaaaagtat cgaaagatag
    28561 tagaataatt gatggccgaa accaaagtaa ctgataagat taaatacaat aagcacacca
    28621 gctgtcgaaa atactgcaaa caataattta attgcatcga cgaagccgga atatctcaga
    28681 atgcctgcgt aagtgtgaaa aagccaaaag aaaaaaagtt gaactgaaat aacaagaatc
    28741 aattcaagcc ccaaaggcaa ttgagttgta tttgaaatga ttgaaagttt tgatgcaatc
    28801 aaatcggaaa accagtatga aatgatagaa gctgccacat caacagccaa caccacccat
    28861 ctgggaaggt acttaaatga aattagatgt gaaaaaatat ccgatttgaa taatgagtga
    28921 gatccatttt tttccatgat gattttctca aatttttata aacaaagtag ataattaatt
    28981 tttcaggtaa ttgttatttg ctatttttca gtgtattacg tttaggctgc aaaaagcgga
    29041 aaaaggaagg cctctacctt cccatccttt tttttaaaaa acatagaaaa tttttaatcc
    29101 tcaagctgca tgttgtgaaa cacattctga acatcttcat cgtcttcgat tttttcgagg
    29161 agtttgttta ctgatgcccg ttgttcttcg gtcagagatt tcacgtcgtt ggggaatcgt
    29221 tcgaattcgg cactgtgaat ctcaaaacca ttttcttcca ggtatttctg aatagtggaa
    29281 tacgatgcaa actcaccata aatgattatt tcgtcttcat cctcttctat ctcgttggct
    29341 ccgtaatcaa tgagttcgag ttccagttcg tcagtcgcta aattctcttt tttggctaca
    29401 tgaaacacac aattgtggtc aaacatgaag agtacagaac ccgaagttcc aagtgaacca
    29461 ccgtgtttgt tgaaataact gcggatgttt cctacggttc tggtcggatt gtcagttgcc
    29521 gtttccacca ctacagctat tccgtgaggt ccatagcctt catataccat ttccttgtag
    29581 tccgattcct cgcgcgaaga tgccttttta atagcccttt ccacattttc tttgggcata
    29641 ttttcctttt tggcttgctg aatgagcacc cgtaagcgcg gattggtatc agcttctggt
    29701 ccgccttcgc gaacagcaat ggtgatttgt tttcccagtt tggtaaaaac acgggccata
    29761 ttgccccatc gtttcatttt tgtggctttt ctgtattcaa atgctcttcc cat
//
LOCUS       DAORKP010000006        29161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_34_length_29161_cov_7.721054, whole genome shotgun sequence.
ACCESSION   DAORKP010000006 DAORKP010000000
VERSION     DAORKP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_29161_cov_7.721054"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..1443
                     /locus_tag="PLN75_00795"
     CDS             496..1443
                     /locus_tag="PLN75_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRG02684.1"
                     /translation="MKTKMYEKYSSGCTDTVLMIEPVAFDYNPETAVNNYFQQKNQQG
                     ESETQQLALAEFRGMVRKLREAGIHVITVQDTPYPHTPDSIFPNNWISFHLNNHVVFY
                     PMFAENRRLERRMDILLEIENQLGRKFKYTDYSKFENENIFLEGTGSIVCDRINRIAY
                     ASISPRTDEALFKKFCREMDFRPVCFQATQEVNGDLLPIYHTNVIMCIADKYAVACLS
                     SVRNSAERETLKRELTESGKEIIEISIEQMNHFAGNMLQLKNNDGDKFVVLSESAYNF
                     LSESQISSLEKFNKLIVAPVPTIEKYGGGSVRCMIAEVF"
     gene            1592..>1944
                     /locus_tag="PLN75_00800"
     CDS             1592..>1944
                     /locus_tag="PLN75_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRG02685.1"
                     /translation="MEMEDTYGAHNYHPLPVVLSRGEGVHVWDVDGKRYYDFLSAYSA
                     VNQGHCHPKIIQALTEQVQKLTLTSRAFYNDKLGVYEKYITEYFGFEKVLPMNTGAEA
                     VETALKLCRKWAYQK"
     assembly_gap    1945..2044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2045..2537
                     /locus_tag="PLN75_00805"
     CDS             <2045..2537
                     /locus_tag="PLN75_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRG02686.1"
                     /translation="EEVKPDILILGKALSGGVYPVSAVLSGHEIMDVIRPGQHGSTFG
                     GNPVAAAAAVAALEVVKDEKLAENALQLGRIFRNEMQELCNNSKIAQSVRGKGLMNAL
                     IINNENHENLAWDVCIRLAENGLLAKPTHTNIIRFAPPLVITYVQLMECISIIKKTIQ
                     EFE"
     gene            2802..4739
                     /gene="yidC"
                     /locus_tag="PLN75_00810"
     CDS             2802..4739
                     /gene="yidC"
                     /locus_tag="PLN75_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRG02687.1"
                     /translation="MDKNTIIGFLLIAAIVIGFTWLSKPSEEEIAQRKRYTDSIALVQ
                     QQQQLAQLVQDSAKQQLAATDSAMLQSDSTAVAQKSDAYGDFSASAFGEEKFFTLEND
                     VLKLILSNKGGRIYSAELKNYKAQNNKPLILFDKDESRFNFVLTTNNNRVVNSSELYF
                     EQQGETMKDSAGNQIMTLRLKTNNPNAYIDFIYTMPADDYMVKYDVKATRMNEVMPMS
                     INLLEMNWKSDIRQQEKGRSFEERYSTLSFRYLAGDKGNLNAAKNDRKNIDNQLKWIA
                     FKDQFFSTVLIADKSFTATSLSSEVLDEGSGYLKSFTSDMAVPFDLTGKEPTGFRIYM
                     GPNHFKTLSAYDKKVPRDEKLYLRELIPLGWLIFGWVNQYFVIPMFNILSRFIGSFGI
                     IILIMTIVIKLILFPLSYKSYMSSAKMRVLKPQIDEINAKIPPEKAMERQKATMALYN
                     KVGVSPMSGCLPMLLQMPLLIAMFSFFPSAIELRQQSFLWAPDLSAYDSILSWKGNIP
                     LISNIFGNHISLFALLMTIASIFSTKLSMSTTQPSPDQPGAGMMKWMMYLMPVMFFFM
                     FNSYASGLTYYYFLSSLFTVVQNEIIKLTIDEKKLLAQLHARGNANSNKPKKSGGFME
                     RLEKMQREQQKAMKENAKRKR"
     gene            4872..5882
                     /locus_tag="PLN75_00815"
     CDS             4872..5882
                     /locus_tag="PLN75_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02688.1"
                     /translation="MRMKFFPFLLVLLIVSSCGRNDRFSIDTSENRTDVKIQRFDSAL
                     ILLDTLNATASVKEIYKAYPVFFRQFITAVDTVQPADTLKVARNLRNFITYPEVRKIN
                     RRVMEVFADVSDIEKEISEAYTYLNHYFPKLPKPDVWFFVSGMSRQIITDERMEAIGI
                     GSDFYLGADYEPYKSLVYDYMLQNMNRRNLSVDLISTLLFNYFRFDSKQNRLIDNMLH
                     RGKVMFMLAVFMPERPLNDIIGYNTTQWNWAVKNEKEIWNTVVSQKDLFSSDLQLIRK
                     YLNDAPFTSPVSQESPGRLGTWIGMRIVESYMNKNKEVTLQQLMQINDYQKLMEESGY
                     KP"
     gene            6020..7126
                     /locus_tag="PLN75_00820"
     CDS             6020..7126
                     /locus_tag="PLN75_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02689.1"
                     /translation="MRKIKTQEKLLQTLKSEKLTKLAFQGLDLSAVEEQLMKIQVSNC
                     LFLDCEMTGKLTRHLFPANYIFPQLDVPFSIYPSGLYNKEKLYKGYDYRKPETYAVTP
                     DKRVYDYYKKMGGSEARNISETLARSLHDHSIYDAKHEFLADYDERKIVAIMGGHKLG
                     RDEALYLQTARLAKNLTESGFLMASGGGPGAMEATHLGAWFAGKGDDELTDAVRMLMP
                     APVYSHPDWLKTAYMVLEKYPETTYKSLGIPTWLYGHELSTPFATHIAKLFENSLREE
                     GLLAIAKGGVIFSPGSAGTMQEFFMDLAQNHYKSYGFASPMIFLGKKYWTDEFPVYPM
                     VKSLIETGKLNEIDLSIYDENEEIINHLATVNQR"
     gene            7131..7826
                     /gene="radC"
                     /locus_tag="PLN75_00825"
     CDS             7131..7826
                     /gene="radC"
                     /locus_tag="PLN75_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRG02690.1"
                     /translation="MEPEKRLTIRDWAEDDRPREKLLNKGAAALSDAELFAILIGSGN
                     KNESAVELCKRILGDNHNNLNELARLSIVDLAKYKGIGEAKAISIVAALEIGKRRKTN
                     EVLERTKITQSSDLFELFEPALLDLNHEEFWVAFMNGANKVLETKRLTQGGMKQTVVD
                     VSMLLRMALERSAHAVAVAHNHPSGENYPSREDLQITQKIKTGCDAIGVRLLDHIIIA
                     GGRYYSFADEGKL"
     gene            7932..8828
                     /locus_tag="PLN75_00830"
     CDS             7932..8828
                     /locus_tag="PLN75_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG02691.1"
                     /translation="MKLIEVKDVVKQYSGHRALDGVSLDVPKGTIYGLLGPNGAGKTT
                     LIRIINCITAPDSGEVLLNGKKLQPEDVRLIGYLPEERGLYKKMKVGEQAVYLARLKG
                     MTKQDALKALKFWFEKFEIQDWWNKKVEELSKGMAQKVQFITTILHKPDLLIFDEPFS
                     GFDPVNTDLLKREIIDLRNQGATIIFSTHNMESVEELCENISLVNKSKIVLQGNVAEI
                     RSAYASDSLFVKTAGVVTPENCNIISQNVKNGIFETTLKKTNGTTNNEILQEIISLTQ
                     VYSFEELLPTMNDIFIRTVTEV"
     gene            8886..10220
                     /locus_tag="PLN75_00835"
     CDS             8886..10220
                     /locus_tag="PLN75_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG02692.1"
                     /translation="MSKIGIIIQREYTSRVMKKSFLLLTFLTPVFMAAMIVLPMWISS
                     MKDDTIKTIVVIDKSKTLHQVLKSNEQYTFIFTDEPVDEYRKKQADGKSRKTSDLTAL
                     LYVSDDPVKNPNAVTIYSEKQINVELKTYLNNELNNYVRDSKLASYNIPDLKKKIESS
                     KGNVTISTIKWDEAGNEKQGSAEMALIIGMLAAFMIYMFIMTYGSQVMSGVLQEKTSR
                     IVEVIVSSVKPFELMMGKIIGIALVGLTQFTIWIVFTLILSSIGTALFGPELSTSAMP
                     DTLQMGQSVTTDASSSEMISEVMAMLSGFDFVKVVVLFIIYFLGGYLLYASLFAAIGS
                     AVDNETDTQQFSMPVTLPIIFSIFIGIYAAQSPDSALAFWGSVIPFTSPVVMMARIPY
                     DVPAWQVLVSLALLIGSFIGSTWIAGKIYRTGILMYGKKVSWSEIWKWIRVK"
     gene            complement(10284..10442)
                     /gene="rpmH"
                     /locus_tag="PLN75_00840"
     CDS             complement(10284..10442)
                     /gene="rpmH"
                     /locus_tag="PLN75_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRG02693.1"
                     /translation="MKRTFQPSNRKRKNKHGFRERMATANGRRVLAARRGKGRSKLTV
                     ADEGRHKF"
     gene            complement(10615..11361)
                     /gene="fabG"
                     /locus_tag="PLN75_00845"
     CDS             complement(10615..11361)
                     /gene="fabG"
                     /locus_tag="PLN75_00845"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRG02694.1"
                     /translation="MKLLEGKTAIVTGAARGIGKAIAMRFAAEGCNIAFTDLVIDENG
                     KATEQEIEALGVKAKGYASNAASFEATHQVVEEIVKDFGRIDILVNNAGITKDGLMMR
                     MTEQQWDAVINVNLKSAFNFVHACTPVMMKQKSGSIINMSSVVGVSGNAGQTNYSASK
                     AGMIGLAKSIAKELGSRGIRANAIAPGFIETEMTHALTEEQRAKWAEAIPLRRGGSPD
                     EVAKVCVFLASDLSSYVSGQVINVCGGMNT"
     gene            complement(11578..12726)
                     /locus_tag="PLN75_00850"
     CDS             complement(11578..12726)
                     /locus_tag="PLN75_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02695.1"
                     /translation="MKKIIQVSALFLIAVLLFSSCEKNKNRTLNFQSAKSDFLGDPTR
                     TSVDFYDLKLYDGNLTNNPNGDGTFAYIQLNTPTTNDNEIKTGSYLPNNSSQARTFTY
                     ETGIWKTDNQGDYVSGSYIGTYKNGQLIQYPVTEGKITVSRNSNYYTVSGLVIADGTQ
                     YDVSYQGQIEFTDMVVPLPETLTHGEIWYQGDPYGNGVKIYSIRLADDNVRISDFAGN
                     GDAMQIELYTPLTATTIIPDGTYPIMVDKVQVNTAIDGYYDSNDKADYGTWYYTADAL
                     SINKGSVSIQYMTGSTYRISFNFTDDYYGYNFSGTYEGELPFVNKTNSPIAVRSAVRG
                     INGQTNSQSAVTDNARGRNERTAGSRTERQTERRESKSAAMPLRVERK"
     gene            complement(12855..13115)
                     /locus_tag="PLN75_00855"
     CDS             complement(12855..13115)
                     /locus_tag="PLN75_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRG02696.1"
                     /translation="MKSGIHPENYRPVAFRDMSNGETFISRSTVATRETEVIDGVEYP
                     LVKLEISSSSHPFYTGKSKLVDTAGRVDKFMSRYAKRNELKK"
     gene            complement(13337..14113)
                     /gene="truA"
                     /locus_tag="PLN75_00860"
     CDS             complement(13337..14113)
                     /gene="truA"
                     /locus_tag="PLN75_00860"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRG02697.1"
                     /translation="MKQRYFIYFSYNGARYCGWQMQPNGISVQEVMQKAISTILRTNI
                     EMTAAGRTDAGVHARCMVAHVDLEHELPAGIDFVSKLNSLLPNDIAVEKTVEVRPDAH
                     ARFDAFSRRYEYHVVLNKNVFSHEFAMRIFHPLDFERMNRAAELLYQYTDFTSFSKLH
                     TDVKTNNCKITYARWTQDNDKWIFTIEADRFLRNMVRAIVGTLLEVGRGKMTPDEFRQ
                     VIEARNRCDAGTSVPAKGLFLVNVEYPEDVFTGQRGSSEF"
     gene            14205..14735
                     /locus_tag="PLN75_00865"
     CDS             14205..14735
                     /locus_tag="PLN75_00865"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRG02698.1"
                     /translation="MKKLFISLALLCVVMGSGAQTNIIKTNPIGFAFGNFNATYERVI
                     SSSSSLVFKGQYNYNLFLMDEIYKVIGLEDVKSGGVGLGYRYYFTHLKKEVPTGFYIN
                     PQASFSFGSAKVDNSSKESLSILGLGAEIGYQFVWSSGFTLDLGIGPMYSIASSNGLS
                     ASGLLPSATLSIGYAF"
     gene            complement(14811..>16274)
                     /gene="cls"
                     /locus_tag="PLN75_00870"
     CDS             complement(14811..>16274)
                     /gene="cls"
                     /locus_tag="PLN75_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRG02699.1"
                     /translation="QTTKLQITKLQTFMHDVVTILLIIFYVYIILSTISVLLMENRNP
                     VKSISWLLVLVLIPFLGLIIYLVLGQDYRKRKMISRKSIQRIAMRPCAATNIKDLDTS
                     NINIDYQKLIYMLKNNSEADAYAHNKIDVYAEGENIFESIFEAISNAKNHIHIEFFIF
                     EDDKISNKLRELLIRKSKEGVRVRMIYDYIGSYKLSKNYLRSLRTAGVYAKPFLPLRL
                     RLRRSKINYRNHRKLIVVDGIIGFTGGINIADRYLYGSELGKWRDTLVRIEGAGVHGI
                     QKLFLIDWYFVERKLITDEKYFPKPAMFNNNILQVVSSGPDTDWESIMQGICTAIMAA
                     DRYVYIHTPYFMPNELVSGCIQMAALSKTEIRLMIPEKSDARLSDLSTSSFLGDMLEA
                     GVRVFKYQNGMLHSKAIVIDDIVSIVGSTNIDDRSFKQNFEVNAFIFDEKTALQLKKL
                     FVDDMENCRELTLDEWNNRRRRQKLKESLARLFSPLM"
     assembly_gap    16275..16374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16454..17509)
                     /gene="ribD"
                     /locus_tag="PLN75_00875"
     CDS             complement(16454..17509)
                     /gene="ribD"
                     /locus_tag="PLN75_00875"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRG02700.1"
                     /translation="MIPKDEKYMARCLHLAKLGCGSVSPNPMVGAVVVYDDRIIGEGY
                     HHRFGGHHAEPEAIASVREKHLLPEATLYVNLEPCSHYGKTPPCANLIVSEKIKRVVI
                     GCPDPNPKVSGRGVKILKDAGIEVEVGILRKESEKLNRRFICFQEKKRPYILLKWAQT
                     SDGFIDTIRKSHSEEALKISNPITQQLTHKMRSENMAIMVSTNTVLLDNPSLTVRYWS
                     GKSPVRIAIDRKGIIPGNFNLKNQKERTIIFTAVASESKPNLEYVQIPDESNDNLPFI
                     LRKLYELNIHSVLVEGGARLLTSFIRAGLWDECNIEISTATAGQGIKAPELTDAEPES
                     ETIIDNHQWKHFCKVRS"
     gene            17674..18435
                     /locus_tag="PLN75_00880"
     CDS             17674..18435
                     /locus_tag="PLN75_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRG02701.1"
                     /translation="MKKLILITLASLALSLSSCNELLQIANTMGNPDIPVSEAENAAG
                     LKSSLDVGIENAVNILGIENGFMNDVLVKILLPAEAKPIIDNIRLIPGGEELVNRAIL
                     SLNRTAEDAVKEATPIFKHAITSITFKDAAGILFGGDYAATSYLKEKTSADLLAAFAP
                     KVKNSLGKPLVANLSTTQSWNALTSAFNKVAASPVGIYGGMKPVNVNLEEYVTQKALD
                     ALFLKVGEEEKKIRENPVARITAILKRVFGQLDKK"
     gene            18506..19510
                     /locus_tag="PLN75_00885"
     CDS             18506..19510
                     /locus_tag="PLN75_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRG02702.1"
                     /translation="MRLLYFVFALMLSVSGGFLFSQSGLVPLDRHLSTINYPFEVHLF
                     EFNTQGQTLTMAYMDVMPDNYNGKNILLLHGKNFNGAYWRTTIQQLTANGYRVVVPDQ
                     IGFGKSSKPTTYQYSFHQLASNTKQLLDTLGIQHTVVLGHSMGGMLATRFALMYPDMT
                     LKLILENPIGLEDYKTLTTWQSLNDAYSAELKNTYESYRKYQLTFYYDNKWKPEYDEW
                     LNMLAGWTLHPDYPVIARTAALTTDMIMTQPVVYEFGDIKVPTLLIIGTRDRTAIGKD
                     RAPKHVQPNMGLYNKLGRKTQRLIPESKLVELKGVGHMPHIETFDLFITSVLNFLNEK
                     "
     gene            19500..19925
                     /locus_tag="PLN75_00890"
     CDS             19500..19925
                     /locus_tag="PLN75_00890"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRG02703.1"
                     /translation="MKNSLDKPETITEYIAQFPPDIQDRLTAIRKLIHELVPEAVEVI
                     SYEMAALKYRKKILVYFAAFKNHIGYYATPDTNSAFGEELLHYKVGKGSIQFPHNEPL
                     PLELIARLTLFKANLIDVKVNTEKTLTKSNSLKNIDSNP"
     gene            complement(20038..21801)
                     /locus_tag="PLN75_00895"
     CDS             complement(20038..21801)
                     /locus_tag="PLN75_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02704.1"
                     /translation="MNKKIFYFLSLPVFISALPVAAQENDTVVHKSVTVTREFQPVII
                     DAGKIITFPTVIEPSVEKTKPVYSDITTPLNIDYNIHLLKPEELVHKPLPAKNGFFRA
                     GFGYPLNTLGDFMYPLLKNDQNRLDVGLHHLGTFGDKVHSKTSGSLQFNHLFDKLELY
                     AGAGGRHDFFNYYARSFAGTDPVIMSDVASRYGDASYSTPEDNSLSLFQLSALPLNDT
                     HWRFNSHVGARSLPLADDLIFDAGVNYDLFKSAGNNLTENRIRINGSFSVPFQKNRLG
                     MDVDINSFMYNKNSASGLNFDEQYSLLKFNPYWMLTGERGYLRLGVKTGISIGYGQVF
                     TPSPDVTAQWNALPEYLALYGGVTGDLTVNSLNRIYNENRYLSPQNRPEDTYTPIDAY
                     AGLKFSPVFNLLIDVYGQYKIINNQYFYINRPYELLVPSASQMPGNMNNFFHNRFDVI
                     YSKAQRATVGMRTAWDFKNQVNVYLKGAYHYWNVDGQAHAWQLPAWDADLGASVKINN
                     DISVNTQFIFQEGRYALLSETDGTRMAPVLDLNLGAAYAYRDWMSVFLKVNNILNKKY
                     ETYSGYDVQGINAMIGAAFSF"
     gene            complement(21967..24996)
                     /locus_tag="PLN75_00900"
     CDS             complement(21967..24996)
                     /locus_tag="PLN75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG02705.1"
                     /translation="MSKKIILAAVFALLIQVAGAQHSMVFTQRDNLFHEGKELFDQRK
                     WAASYRNFEEFLKNTETTNAGMIQEAAFYMASGAYELRQKNAYDLLLQYLDNHPYTPY
                     LDRIQLMLGMLEYESRNYDKALAYFRQIDETHLVERDRTDYLFSRGYANLEINDIPKA
                     LDIFKTLKSMNSAYQPAARYYTGYSQYRLGNYDEALTDLLAVEKNPEFEDIAPYYIAQ
                     IYYFKRNFGEMEKRAEMLIRKYPDNINNAEIYRMTGEKAYADGNYTKAIDNLKKYENL
                     FPQVLRNDMYYLGISYLKTGKPADAVKYLSQVTIEPDEMTESAYLQLGNAYVAMGNKE
                     NARLAFEAATQTNFNTKVREEALFNYALTTYETNTAFGESVKAFELFIREFPNSANID
                     KAYDYLSTVYLTSKNYSAAYQSVSNIKTLTPKLRETKQYLEYQLGTESFAESNYQRAI
                     EFFTKAIQSAPTGRYLADSYFWRGESHYRSRDYVRSVSDLNSFFSQPNVMSNPNYTQA
                     HYALGYAWFSQKKYNESLSSFLKYVNEEKNSSTPTYADALNRIGDAYFSNRNFTKASE
                     YYEKVMNTTYNGDYALFQSAYMTGLNKNYNQKISKLEDLLNKYPNSEYGDDALYEIAR
                     AYLMLENNAKTIETYNRLIAGYPNSQLAPKALLETGMVYFNQHEFERAIPAFKKVVAE
                     YPASEEADTALESLETIYINTNKVGDYLDYTKSIGKNIESGSMARADSILFVAAEKQY
                     MAGNHAEAAAGLHSYLSRYCPGGKFCTIAQYYLADSYYTLGDKLKALDEYIKILDSRG
                     NPYTEEAALRAAGIAFDNKDYTASMAYFKRLEQVAQTTENKNIARLGVLRTSFFLNNT
                     DETIQIAGEIIADSKSTAQMRTEALLNRAKALSRQNKFNEALKDLKEIKIDTRTSLGA
                     EAKFALSETYFKLNKLNDSEKEVLDFAKKSTPHQYWLARSFIILSDIYTQKGDDFQAK
                     QYLQSLQKNYTAKDDVQEMIAVRMAGIEKRAKERVIK"
     assembly_gap    25028..25127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25128..25597)
                     /locus_tag="PLN75_00905"
     CDS             complement(<25128..25597)
                     /locus_tag="PLN75_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRG02706.1"
                     /translation="MKFSEKFKFCPACGSDKYIIHNVKSKRCDICGFIYYQNPSAATA
                     AFIRNEDGDLLVCRRSHEPAKGTLDLPGGFIDYDETAEEGIAREVNEELGIRMDNLRY
                     VFSLPNDYLYSGLNVPTLDMFFEGKVANNVEIHAADDVEECFFLPVREINPRLFG"
     gene            25797..26033
                     /locus_tag="PLN75_00910"
                     /pseudo
     CDS             25797..26033
                     /locus_tag="PLN75_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
     gene            26083..27726
                     /locus_tag="PLN75_00915"
     CDS             26083..27726
                     /locus_tag="PLN75_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRG02707.1"
                     /translation="MVSVDGLAVEFGGTTLFSDVSFVINPKDRIALMGKNGAGKSTLL
                     KILAGVRDPSRGKVSMPRDFNIAYLPQHIKTNDSRTVFEETAQAFAQLHELESRIAYL
                     NEQLTIRSDYESDEYMQLIEEVTALSEKFYSIEETNIDEHIEKTLLGLGFEREDFDRN
                     TAEFSGGWRMRIELAKILLQNPDVILLDEPTNHLDIDSVQWFEDFLINTAKAVVLISH
                     DRAFVDTVTTRTIEVTMGRIYDYKATYTHYLELRKERREQQQKQYDEQQKMIAETQEF
                     IERFKGTYSKTLQVQSRVKMLEKLQIIEVDEQDTSALRLKFPPSPRSGNYPVIAEGVG
                     KAYGEKRVFANADYTIERGQKVAFVGRNGEGKSTMVKCIMGETEHEGKLTLGHNVKIG
                     YFAQNQASLLDEDLTVFQTIDDIAVGDIRTKIRDILGAFMFGRDDVDKKVKILSGGER
                     TRLAMIKLLLEPVNLLILDEPTNHLDMRTKDILKQALTDFDGTLIVVSHDRDFLDGLV
                     TKVYEFGHGKVTEHLEDIYGFLAKKKMESLREIESKSKK"
     gene            complement(28024..28692)
                     /locus_tag="PLN75_00920"
     CDS             complement(28024..28692)
                     /locus_tag="PLN75_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02708.1"
                     /translation="MRTRLFILGTIAAMLLSCGQKADKNSSNPDATAKEIAQEIADTK
                     SYYIEYKTTMDMQGMKSTSVSKQWIDIKNDRLVMETETENEMMGQKTKESALTIGKDG
                     YTYVISLTNKTGLKMKSDGNEEDPMDNIKPDDNETFRQMIEKEGGKIIGNEDFLGKNC
                     IVIEMTSEGTTMKMWYYKGIPLKMTSDAYVMEAVKFEEGISIPDSKLEIPEDITISEM
                     PVMN"
     gene            complement(28744..>29161)
                     /locus_tag="PLN75_00925"
     CDS             complement(28744..>29161)
                     /locus_tag="PLN75_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02709.1"
                     /translation="WDNFYIIVNMSELEDNCMTIVNDDRYTFPYACDGGVVSHKGGKF
                     NPRLQLIQANSTFATEMVDADLILCSSDTHNPADLRINCYMGFDNHGQQFNIPIADKV
                     QTIKKRKPFTVTATETNEEVPSYSAKLKIMFFPVWK"
BASE COUNT         7954 a         6226 c         6388 g         8293 t
ORIGIN      
        1 attttgtcga aaagtcgcaa tacaatgccg aaaagaagta atattttgtc gaaaatgctt
       61 tatgaatccc gtttttcgca ataggaatct tgaaaacccc gctactttta cttcccaaaa
      121 agttacagta tgcaaaaaac cgggtacttt tgacttctgt aatattacag aacacgaaaa
      181 accgggtaat ttgtaataaa taaatgattc tgcacagtac cgtattttta tacttattat
      241 ttgcttttca catgtcaatt atctatcttt gccataatga aagcgttcat ttcctcaatc
      301 aaattcgatg cagacaatac acatctgctt gttttcaaat cgtaatcctt atttaggatt
      361 acgcatatta cttcctgact ttttcaacag aatatttttt cagattaaac attttcagca
      421 taaataacaa acagtagaaa attaaccttc atataatccg attttcatca tttatttacc
      481 cgaagttcca tgaatatgaa aaccaaaatg tacgaaaaat acagttccgg ctgtacagat
      541 acagttctga tgattgaacc cgtagcattt gactacaatc ccgaaactgc tgtaaataat
      601 tatttccagc agaaaaatca gcagggtgaa agtgaaaccc agcaacttgc actggccgaa
      661 ttcaggggaa tggttcggaa actccgggag gccggaattc atgttatcac ggtgcaggat
      721 acaccctatc cgcacacacc ggattccatt tttccgaaca actggatctc gtttcacctc
      781 aacaatcacg tggtctttta tccgatgttt gccgaaaacc gccgtctgga acggcgaatg
      841 gatatcctgc tcgaaattga gaatcagctg ggccgtaaat tcaaatacac tgattattca
      901 aaattcgaaa atgaaaatat tttcctcgaa ggaacaggaa gcattgtttg tgaccggata
      961 aaccgcatag cgtatgcgag catttcaccc agaaccgacg aagcactttt taagaaattt
     1021 tgcagggaaa tggacttcag accggtatgt tttcaggcca ctcaggaagt gaacggtgat
     1081 ctcctgccca tttatcacac caatgtaatc atgtgtattg ctgataaata tgctgttgct
     1141 tgtctttcgt ctgtgagaaa ttcggccgaa agagaaacgc tgaagagaga actcacagag
     1201 tccggaaaag agatcatcga aatttcgatc gaacagatga atcactttgc cggaaatatg
     1261 ctacagctga aaaacaacga cggagacaaa tttgtcgtgt tgtcagaatc agcttataat
     1321 tttttgtctg aatcacaaat cagctcgctg gaaaaattca acaaactgat tgtagcaccg
     1381 gttccgacca tcgaaaaata cggtggcgga agtgtcagat gcatgattgc agaagtgttt
     1441 tgaaaaagcc cctcattgat ttgcacctca ttttaaataa aaaaactgat aaatatacca
     1501 tacaataatt attatgaatt ccgacaacaa tcaatccggc caatcttttg aaaagtacta
     1561 cattgaatcc cggcatacag cttcagcgct tatggaaatg gaagatactt acggtgcgca
     1621 caattatcat ccgcttccgg tggtgctcag cagaggcgaa ggagtgcatg tgtgggacgt
     1681 ggacggtaaa aggtattacg attttctttc ggcctattcc gccgtaaatc aggggcattg
     1741 ccacccgaaa atcattcagg cactgacaga acaagttcaa aaactgacgc tcacatcccg
     1801 tgcattctac aacgacaaac tgggcgttta cgaaaagtat atcacagaat acttcggttt
     1861 cgaaaaagtg ctgcccatga ataccggagc cgaggctgtg gaaacagcac tgaaactctg
     1921 ccgaaaatgg gcctatcaga aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnntgagga agtaaaaccc gatattctca tactcggaaa ggcattatca ggtggagttt
     2101 atccggtttc ggcggtgctt tcgggccatg aaatcatgga tgtaatccgt cccggccagc
     2161 acgggtccac attcggaggt aatcccgtag ctgctgcagc agccgtggct gctctcgaag
     2221 tggtgaaaga cgagaaactg gcagaaaatg cactgcaact cggccggatt ttcagaaatg
     2281 aaatgcagga actttgcaat aattctaaaa tcgctcagtc agttcgcgga aaaggattga
     2341 tgaacgcgct gataatcaac aacgaaaatc acgaaaacct ggcctgggac gtttgtatcc
     2401 ggttggccga aaacggactg ctggctaagc ctacgcatac caatatcata cgctttgccc
     2461 cgccgctggt aattacctac gtgcagctca tggaatgcat ttccattatc aaaaaaacca
     2521 ttcaggaatt tgagtaaaaa acgtgaatga ccgaaccatt catcgcaaga aatggttaca
     2581 cattttcaaa aaaagcggga aatgaaccta ttcccgcttt tttattttcc gtttactcac
     2641 agatttactt tctatattca gttttttttc cgaaatttgt acccttaatt tttgatggcc
     2701 actgaggctg aaaaatttca aatcaacaga ataatactta actcaacggg tttagaatga
     2761 gtcttctttt ccggactttc taaactcgaa attttgtaaa tatggataaa aatacaatta
     2821 taggatttct tctcattgcc gctatcgtaa taggtttcac ctggctcagc aaaccatcgg
     2881 aagaagaaat agcccaaaga aaaagataca ccgactccat cgctttggta cagcagcaac
     2941 agcagctggc gcagttagtg caggactcgg caaagcagca gcttgctgca accgactccg
     3001 cgatgcttca gagtgattcc acggctgtgg cacagaaatc agatgcttac ggtgatttca
     3061 gcgcatcggc ttttggcgaa gaaaaattct ttacgctcga aaatgatgta ttaaaactga
     3121 tactgagcaa caaaggtggg cggatatatt ctgccgaatt aaagaattat aaagctcaga
     3181 acaacaagcc attgattctt ttcgataaag acgaaagcag attcaatttt gtacttacta
     3241 ccaacaataa ccgtgtggta aattcgtccg agctctattt tgagcagcag ggtgaaacga
     3301 tgaaagacag cgccggaaac cagatcatga cattgcgcct gaaaaccaac aatccgaatg
     3361 cctacatcga ctttatctat acgatgcctg ccgatgatta catggtgaaa tacgacgtga
     3421 aagcaacccg gatgaacgag gtgatgccca tgtccattaa tttgctggaa atgaactgga
     3481 aatccgatat tcgtcagcag gaaaaaggcc gttcgttcga ggaaaggtat tccacactca
     3541 gtttcaggta cctggcaggt gataaaggta acctgaacgc cgccaaaaat gaccggaaaa
     3601 acattgataa tcagcttaaa tggattgctt tcaaagatca gtttttcagt acggttctta
     3661 ttgccgataa atcattcacg gccacatcac tctcaagcga ggtgctcgac gaaggctccg
     3721 gatacctgaa gtcattcacg tcggatatgg ctgtaccttt cgatctgacc ggaaaagaac
     3781 ccaccggatt ccgtatttat atgggcccca atcatttcaa aacactctca gcttacgata
     3841 aaaaagtgcc acgcgatgaa aaactctatc ttcgcgaact cattccgctc ggctggttga
     3901 tcttcggatg ggtaaaccaa tactttgtaa ttccgatgtt caatattttg agccggttta
     3961 taggcagttt cggtatcatt atcctgatta tgaccattgt cattaaactc atactgtttc
     4021 cgctgagtta caaatcctac atgtcgagcg ccaaaatgcg ggttctgaaa ccccagattg
     4081 acgaaataaa tgctaaaatc ccacctgaaa aagccatgga acgccagaag gcaacgatgg
     4141 ctctctacaa taaagtgggt gtgagcccaa tgagcgggtg tttgcccatg ttgctgcaga
     4201 tgccgctgct catagccatg ttcagcttct tcccgtcggc catcgagctg cgtcagcaaa
     4261 gtttcctgtg ggcacccgac ctctcggctt acgactccat tctttcctgg aaaggcaata
     4321 taccgcttat cagcaatata ttcggcaatc acatcagtct ttttgcattg ctgatgacta
     4381 tagcgagtat attctccaca aaattaagca tgtcaaccac acagccatca cccgatcagc
     4441 ccggtgccgg catgatgaaa tggatgatgt atctgatgcc cgtcatgttc ttctttatgt
     4501 tcaacagtta cgcttccggt ctgacgtatt attacttcct gtcgtcgttg ttcacagttg
     4561 ttcagaatga aattatcaaa ctaaccatcg acgagaagaa actgcttgcg caacttcatg
     4621 ccagaggcaa tgccaacagc aataaaccca aaaaatcggg tggtttcatg gaacggcttg
     4681 aaaaaatgca acgcgaacag caaaaagcaa tgaaagaaaa tgccaaacga aaaagataat
     4741 tcagccggct gaaaagataa gtcaaattac tttcagccaa aaattaagtc aaaccgcaaa
     4801 gctttacagg gatttttttt ataactttgt aaaactttgc ggtttacttt tttaaaaaaa
     4861 caaattcaag catgcgaatg aagtttttcc cgtttctgtt agtattatta atcgtttcat
     4921 cctgcgggcg aaacgatcgt ttcagcattg atacctcaga aaaccgcaca gatgtgaaaa
     4981 tacagcgttt tgactccgca ctgattctgt tggatacact taacgccact gcttcagtaa
     5041 aagaaatcta caaagcatat ccggttttct tccggcagtt tatcaccgcc gtagatacgg
     5101 tgcagcccgc tgatacgctt aaagtggccc gcaatctgag aaactttatc acctatcctg
     5161 aagtgaggaa aatcaaccgg agggtaatgg aagtatttgc tgacgtatcg gatatcgaaa
     5221 aggaaatatc ggaggcatat acttacctca atcattattt tccgaaactg ccgaaacccg
     5281 atgtctggtt ttttgtttcc ggaatgagcc ggcagataat caccgacgaa cggatggaag
     5341 ctatcgggat aggaagtgat ttttatctgg gagctgatta tgagccatac aaatcgctgg
     5401 tatacgatta tatgcttcag aatatgaatc gacgcaatct ttcggttgat ctgatttcca
     5461 cgctgctttt caactatttc aggttcgaca gtaagcaaaa cagactgatt gacaacatgt
     5521 tgcatcgtgg caaagtgatg ttcatgctcg cagtatttat gcccgaaagg cctctgaatg
     5581 acatcatcgg atacaatact acgcaatgga actgggcagt aaaaaacgaa aaagaaatct
     5641 ggaataccgt cgtttcgcag aaagatcttt tttcatccga cctgcaactt atccgcaagt
     5701 atctgaatga tgcacctttc acttcgcccg tttcacagga atcgcccgga cgactcggta
     5761 cgtggatcgg aatgagaatt gtggaaagtt atatgaacaa aaataaagaa gtgacgttgc
     5821 aacaactgat gcaaatcaat gattatcaaa aacttatgga agaatcggga tataagccgt
     5881 aaaccggtgg atcggcactc ttttttattc atacaactca ttttttataa gtgtcagtca
     5941 tgttttaaaa aaaaatgatg attggttgat gagtaggatt gcaggtagct aaatgtattt
     6001 tttttaaaaa tagggattta tgcgaaaaat aaaaacacag gaaaaactgt tgcagacgct
     6061 gaaaagcgaa aaactgacaa agctcgcttt tcagggactc gatttgagcg cggtagaaga
     6121 gcagctgatg aaaattcagg tgagcaactg tctttttctc gattgtgaaa tgacgggaaa
     6181 acttaccagg catctttttc ctgctaatta catatttccg cagctggatg tgcccttcag
     6241 catatatccc tcaggattgt acaacaagga aaaactctac aaagggtacg attaccgcaa
     6301 gcctgaaacc tacgccgtta ctccggacaa gagagtgtat gattattata aaaaaatggg
     6361 cggatcggaa gcgcgaaaca tcagcgaaac ccttgcccgc tcactccatg atcattccat
     6421 ctacgatgcg aaacacgaat ttctggctga ttacgacgaa cgtaagattg tggcaatcat
     6481 gggaggccat aaactcggac gcgatgaggc gttatacctg cagacagccc ggctggcaaa
     6541 aaatcttacc gaaagcggtt ttctgatggc atcgggtggg ggacccggag ctatggaagc
     6601 tactcacctc ggagcgtggt ttgccggaaa aggcgacgat gaacttactg atgcagtccg
     6661 tatgctgatg cctgctccgg tatattcgca tcccgactgg ctcaaaacgg cctatatggt
     6721 gcttgagaaa taccccgaaa ccacatataa aagtctggga attccgacct ggctctacgg
     6781 tcatgaacta tctactcctt ttgccaccca cattgccaaa ttattcgaga atagtctgcg
     6841 cgaagaagga ctgctcgcca tagccaaagg aggtgtgata ttttcacccg gaagcgccgg
     6901 aactatgcag gaatttttca tggatctggc ccaaaatcat tataaaagct atggttttgc
     6961 cagtccgatg atattcctgg ggaaaaaata ctggacagat gaatttccgg tttatccgat
     7021 ggttaaatcg ctgattgaaa ccggcaaact gaacgaaatt gatttgagta tttatgatga
     7081 aaatgaggaa atcataaatc atctcgctac tgtcaatcaa cgataataac atggaacctg
     7141 aaaaaagact aacaatcagg gattgggccg aagatgaccg tccccgcgag aaactgctca
     7201 acaagggagc agcagccttg tcggatgccg aattatttgc catactcatt ggctccggaa
     7261 acaaaaacga aagtgctgtg gaactttgca agcgtattct cggcgataac cacaacaacc
     7321 tcaatgaact cgccagactg agcattgtcg atctggccaa atacaaggga ataggagaag
     7381 ccaaagccat cagcattgta gcagcgctgg aaataggcaa acgacgaaaa acaaatgaag
     7441 tgcttgaaag gacaaaaata actcaaagca gtgatttgtt tgaactgttt gaacctgctt
     7501 tactggacct gaatcacgag gaattctggg tggcattcat gaacggagcg aataaagtgc
     7561 tggaaaccaa acgactgact cagggtggaa tgaaacaaac ggtggtagat gtatccatgc
     7621 tgctccggat ggcgctggag cgttcggcac atgctgttgc agtggctcat aatcatcctt
     7681 cgggcgaaaa ttatccgagc agagaagatt tacaaatcac ccaaaaaata aaaaccggct
     7741 gcgatgccat cggtgtgagg ctgctggatc acatcatcat tgccggagga agatattaca
     7801 gctttgcaga cgaaggtaag ttgtagaaag gttagttctc caaaaatatc taaatcaatg
     7861 tgccaatttg ccaatttgcc aataaactaa tatactaata tactaataaa ctaatacacc
     7921 aattaactaa attgaaatta atagaagtaa aagacgtcgt aaagcagtat tccggacacc
     7981 gcgcattgga cggagtgagt ctggatgttc caaaaggaac aatttacggt ttgctcgggc
     8041 cgaacggtgc cggtaaaacc acgctcatcc gcatcatcaa ttgtattact gctcccgata
     8101 gtggcgaagt gctgctcaac gggaaaaaac tccagccgga agatgtccgt ctgatcggat
     8161 atctgcctga agagcgcgga ctgtacaaaa aaatgaaagt gggcgagcag gccgtttacc
     8221 tggcacgcct caaggggatg accaaacagg atgccctgaa agcattgaaa ttttggttcg
     8281 agaaatttga aatacaggat tggtggaaca aaaaggtgga agaactctcc aagggaatgg
     8341 ctcagaaggt gcagtttatc actaccattc tccataaacc cgatttgctt attttcgacg
     8401 aaccattcag cggtttcgat cccgtaaata ctgacttgct aaagcgcgaa atcatcgatt
     8461 tgcgtaatca gggagctacc atcatctttt ccacacacaa catggaatcg gtggaagaac
     8521 tctgcgaaaa tatctcactg gtcaacaaat caaaaatagt gctgcagggc aatgtggcgg
     8581 aaatacgatc agcttatgcc tcagacagct tgtttgtgaa aacggccggt gtggttacac
     8641 ctgaaaactg caacattatt tctcaaaatg tgaaaaacgg cattttcgaa accacactta
     8701 aaaaaaccaa cggcacaact aacaacgaaa ttttgcagga aatcatttcg ctgacgcagg
     8761 tttattcttt cgaagaattg cttcctacga tgaatgatat ttttataaga acggtaacag
     8821 aagtctgacg cacgaagcac aaagaaattc taattaacaa attaactact caaccataca
     8881 accaaatgtc caaaatagga atcatcatac agcgtgaata cacatcgcgc gtaatgaaaa
     8941 agtctttttt gttgcttact tttctgacac cggttttcat ggcagccatg atcgtgttgc
     9001 ccatgtggat ttcgtccatg aaagatgata caataaaaac cattgtggtt atcgacaagt
     9061 cgaaaacact tcatcaggtg ctcaaaagca acgaacaata tacatttatt tttaccgacg
     9121 agccggtaga tgaatacaga aagaagcagg cagacggcaa aagccggaaa acaagcgatc
     9181 ttaccgcact gctttatgta tcggacgatc cggtgaagaa tccgaatgcg gttaccattt
     9241 actccgaaaa acagattaac gtagagttga aaacatatct gaataatgaa ctgaataact
     9301 atgtgcgcga cagcaaactg gcttcataca atatccccga tttgaaaaag aagatagagt
     9361 cgtcaaaagg caatgtaact atctccacca tcaaatggga cgaagccggc aacgaaaagc
     9421 agggctctgc cgaaatggca ctcatcatcg gtatgctggc agcattcatg atatatatgt
     9481 ttatcatgac ctatggttcg caggtaatgt cgggagtact gcaggagaaa acaagccgca
     9541 ttgtggaggt gattgtctcg tcggtgaagc cttttgaact gatgatggga aaaatcatcg
     9601 gcattgcgct ggtggggctc acccagttta ctatctggat tgtatttaca ctcattctca
     9661 gtagtatagg tactgccttg tttggtccgg aattaagtac ctcagccatg cccgataccc
     9721 ttcagatggg tcagtcggtt acaaccgatg ccagttcttc tgaaatgatc agcgaggtaa
     9781 tggccatgct ttcgggattt gattttgtaa aagtagtggt gttgtttatc atctacttcc
     9841 tgggtggata cctgctctat gcatcgcttt ttgcggctat cggctctgcg gtggacaacg
     9901 aaaccgatac gcagcagttc tccatgccgg taacgctgcc catcattttc tccattttta
     9961 tcggcatcta cgccgctcaa agtcccgata gcgcactggc gttctggggc tcggtcattc
    10021 cgtttacatc gccggtggtg atgatggcgc gtatacctta cgatgtacct gcctggcagg
    10081 tgctcgtttc gctggcattg ctcataggaa gttttatagg atcaacctgg atagccggga
    10141 aaatttaccg cacaggtatt cttatgtatg gcaaaaaagt gagctggagt gagatatgga
    10201 aatggatccg cgtgaaataa acaaaaaaat cagaacccgc aaaggttctg atttttttat
    10261 gattgtactg aatgtgagat gaattagaat ttatgacgac cttcatcagc aactgtcagt
    10321 ttggatctgc cttttccacg acgagcagct aatactctgc gaccgtttgc agttgccatt
    10381 ctttcgcgaa agccgtgttt gttctttctt tttctgtttg agggttgaaa tgtccttttc
    10441 attgctattt agtgttttta taatttttct tttcggactg caaagttagt caaaatagtt
    10501 tagtctgcaa atatttatct gtaaattttc caaaataatt cacgtacctg ttttgccgcc
    10561 gtcatttgat atccgtcatc ttcagaactc aaaaactcag aactcagaac taatttacgt
    10621 attcattccg ccgcacacat tgatcacttg cccgctaaca tagctggaga ggtcggatgc
    10681 gaggaataca cacaccttgg ccacttcgtc gggtgagcca ccgcggcgca gagggatggc
    10741 ttcggcccat ttggcacgtt gttcttccgt cagagcatgc gtcatttcgg tttcgatgaa
    10801 tcccggagca atggcgttgg cacgtatgcc gcgcgatccc agttccttgg cgatagattt
    10861 agcaagaccg atcattccgg cttttgatgc ggagtagttg gtttgtccgg cattgcccga
    10921 tacgcctacc acagaactca tattgataat cgaacccgat ttctgcttca tcatcacggg
    10981 tgtacaggcg tgtacaaagt taaatgccga tttcagattt acgtttatca cagcatccca
    11041 ctgctgctcg gtcatgcgca tcatgagtcc gtctttggtg attccggcgt tatttaccag
    11101 tatgtcaatc cgaccaaaat ccttcactat ttcttccact acctggtgag ttgcttcaaa
    11161 tgaagctgca ttggaggcat atcctttggc ttttacgccc agtgcttcta tttcctgttc
    11221 ggtggctttg ccgttttcgt cgattaccag atcggtaaaa gcaatgttgc agccttcggc
    11281 agcaaaacgc atggcaatgg ctttgcctat tccgcgtgca gcgccggtta cgatagccgt
    11341 ttttccttct aaaagtttca tgtgtaaata atttgttttt tagttgtcac atggaactcg
    11401 catgtcagat tttcatcatc tttgttgaac caccgtgttc atgctcgttt catgttttta
    11461 ttcttttttg ggtttaatcg gctgcaaaga tactacttat tggatgttta ttttcaaaat
    11521 tttttataaa aaaagggaat gaaagttcat tcccctgatt tatgcttcct gaaaaatcta
    11581 ttttctttca actctgagtg gcattgcagc gctttttgat tctcttctct cagtctgccg
    11641 ttcggttctg ctgccggctg ttctttcgtt tcttcccctt gcattgtccg ttacagccga
    11701 ctggctgttg gtttgtccgt ttatgcctct tacggccgat ctcactgcta tcggtgaatt
    11761 ggttttgttt acgaaaggaa gttcgccttc ataagttccg ctgaaattat acccataata
    11821 gtcgtcggtg aaattgaaac ttatcctgta tgtacttccg gtcatatact gtatgcttac
    11881 tgagcctttg ttgatgctca gagcatcggc tgtgtaatac catgtgccgt agtcggcttt
    11941 gtcgttggag tcgtaatagc catctattgc cgtatttacc tgtactttgt caaccattat
    12001 ggggtacgta ccatccggaa taatggtggt ggcggtaagc ggagtataga gttctatctg
    12061 catggcatcg ccgttgccgg caaaatctga aatcctcaca ttgtcatcgg caagcctgat
    12121 ggaatatatt ttcactccgt ttccgtaagg atcgccctga taccagattt caccgtgagt
    12181 gagcgtttca gggagaggca ctaccatgtc ggtaaattct atctgaccct gataagatac
    12241 atcgtactgt gttccatcgg ctatcacaag gcccgaaact gtataataat tgctgttgcg
    12301 tgaaaccgtg attttccctt cagtaaccgg atattgtatc aattgaccgt ttttataagt
    12361 gcctatgtag cttccgctta cgtaatctcc ctggttgtcg gttttccaga tgcctgtttc
    12421 gtacgtaaag gtacgtgcct ggctcgaatt attgggcaga taacttccgg ttttgatttc
    12481 attatcattt gttgtaggtg tgttgagctg tatgtaagca aaagttccat caccgttcgg
    12541 attgttggtc agattgccgt cgtaaagttt caggtcgtag aaatcaacac ttgtccgggt
    12601 gggatcaccc agaaaatccg atttagccga ctgaaaattc aatgtgcggt ttttgttttt
    12661 ctcgcaggat gaaaacaaca aaactgcgat taaaaacagc gctgaaacct gtataatctt
    12721 tttcatggag cacgtaatga ttaatttgtt aatactaaaa atctgttttt atcagttagt
    12781 caaaaataat gccaattatt taaaacaaaa aaccctgatt ttttatcagg gatttctgta
    12841 tgttaccgtt gaaattactt tttcaattca ttccgttttg cataacggct catgaattta
    12901 tccacacgtc ctgctgtatc caccagtttg gattttccgg tgtagaatgg gtgtgatgaa
    12961 cttgaaattt ccaattttac caaaggatat tccactccgt caattacttc tgtttctctg
    13021 gtagcaactg tagagcgtga aataaatgtt tcgccatttg acatgtctct gaatgccacc
    13081 ggacgataat tttcaggatg tattccgctt ttcatatatt cttgtattat tttatttctt
    13141 tttattttgg cttgcaaaga tacaaataat aattgatgca gcaaattttt ctttggtatt
    13201 ttatttaaaa aatctgctgc aggtttaatt gaacgtacaa aatttacggt tttcaataca
    13261 cagaactcag aacatgtaaa ttagaaatta gaaattagaa attagaactc agaagtcaaa
    13321 actcaaaact cagaactcaa aactcagaac tccctctttg tcctgtaaaa acgtcttcag
    13381 ggtattccac attcaccagg aaaaggcctt tggcgggaac ggaagtgccg gcatcacatc
    13441 tgtttcgggc ttcaattacc tgtctgaatt catccggtgt catctttcct ctgcctacct
    13501 ccagcaaggt gcctacgata gcccgtacca tgtttcgcag aaagcgatcg gcttcgatgg
    13561 tgaaaatcca cttatcgttg tcctgagtcc aacgggcata cgttatttta cagttgttgg
    13621 ttttcacatc ggtgtgtaac ttgctgaaac tggtgaaatc cgtgtactga tacagcaact
    13681 ctgctgcgcg gttcatcctt tcaaaatcaa gcggatgaaa tatgcgcatg gcaaattcgt
    13741 ggctgaatac attcttattg agcaccacgt gatattcata cctcctggaa aaggcatcaa
    13801 aacgggcgtg cgcatcgggt cttacctcta ctgttttctc cacggcgata tcgtttggca
    13861 gcaggctgtt cagcttgctt acgaaatcaa tgccggcagg aagttcgtgt tccagatcaa
    13921 catgtgccac catgcagcgt gcatgcacac ccgcgtcggt tcggccggcg gctgtcattt
    13981 caatgttggt gcgcagtatg gtcgagattg ctttttgcat cacttcctgc accgaaattc
    14041 cgttgggctg catctgccaa ccgcagtacc gggcgccgtt gtaggagaaa tagatgaaat
    14101 acctttgttt catattgcaa agttaataaa aattgaattt aaaatggatt tctcaatata
    14161 ttgttctata tttgtcggac aaaattctaa aaaatatgat ttatatgaaa aaactgttca
    14221 tttctcttgc gctgttgtgc gttgtgatgg gttcgggtgc acaaaccaac attatcaaga
    14281 ccaatcctat tggcttcgcg tttggaaact ttaatgctac ctacgaaagg gttataagtt
    14341 caagttcatc attggttttc aaggggcaat acaattacaa tttattttta atggatgaaa
    14401 tatataaagt aattggtttg gaggatgtaa aatccggagg tgtcggactt ggttaccggt
    14461 attactttac gcatctgaag aaggaagtgc ccaccggatt ttatataaat cctcaggctt
    14521 cattttcatt cgggagtgcc aaagtcgata atagttcaaa agaaagttta tcaatactcg
    14581 gattgggtgc cgaaatcgga tatcaatttg tatggtccag cggatttacg ctcgatttgg
    14641 gtattggccc gatgtacagc attgcgtcaa gcaatggttt atcagcatcc ggtttactcc
    14701 cttcagctac tctgtcaatc ggttacgcat tctgatgaaa caaaaaataa acaataaaaa
    14761 aaagcgaacg gaaatgttcg cttttttttg tgcagtaaga ctaacttttt ttacatcagc
    14821 ggactgaaga gcctggccag tgattctttc agcttctggc gtctgcggcg gttgttccac
    14881 tcgtcgaggg ttagttctct gcagttttcc atatcgtcaa cgaacaattt tttgagttgg
    14941 agcgccgttt tttcatcgaa aatgaaggca ttcacttcaa agttctgctt gaaactgcgg
    15001 tcgtcgatat tggtggagcc aacgatagac acgatgtcgt cgattacaat tgctttgctg
    15061 tggagcatgc cgttctggta tttgaagacc ctcacgccgg cttcgagcat atcgcccagg
    15121 aaactcgaag tgctaaggtc ggatagccgt gcatccgatt tttcgggaat catgaggcgg
    15181 atttccgttt tgctcagtgc agccatctga atgcagccgc tcaccagttc gttgggcatg
    15241 aaatagggag tgtgaatgta cacgtagcgg tcggcagcca ttatggccgt acaaattccc
    15301 tgcatgatgg attcccagtc ggtatcgggt cccgatgaaa cgacctgcag gatgttgttg
    15361 ttgaacatgg caggttttgg aaaatatttt tcgtcggtaa tcagttttct ttccacaaaa
    15421 taccagtcga tcaggaaaag tttctgaata ccgtgtacac cggctccctc aatacgcacc
    15481 agtgtatcgc gccatttgcc caattcactt ccgtagaggt agcggtcggc tatatttatt
    15541 ccgccggtga agccgatgat tccatccacc acgatgagtt tgcggtgatt ccggtagttg
    15601 attttgcttc ttctcaacct caggcgcagg ggcaggaatg gcttggcata cacgccggct
    15661 gtacgcagcg aacggaggta atttttggat agtttataac tgccgatgta gtcataaatc
    15721 attctcacac gcactccttc cttacttttc ctgattagca gttcgcgcag tttgtttgag
    15781 atcttatcat cttcaaaaat aaaaaattca atgtgaatat gatttttggc attgcttatt
    15841 gcttcgaaaa tggactcaaa gatattttca ccttcggcat aaacatcaat cttgttgtga
    15901 gcataagcat ctgcttcgct gttgtttttc agcatgtaga taagcttctg gtaatcgata
    15961 ttgatgttgc ttgtgtccag gtctttgata ttggtagctg cacagggacg catggcaata
    16021 cgctggatac tttttcgcga aatcattttt cttttccggt agtcctgccc aagaaccaga
    16081 taaatgatca gaccgagaaa gggaatgaga acaagcacca gcagccacga aatggatttc
    16141 accggattgc ggttttccat caacagcacc gagatggtcg acagaatgat gtaaacataa
    16201 aatatgatta ataatatggt aactacgtcg tgcatgaatg tttgtagttt ggttatttgt
    16261 agttttgttg tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgat
    16381 tgtttggttg tttgtttgat attccttact tcttacttct tacttcttac ttcttacttc
    16441 taatttctaa tttctaactt ctaactttgc aaaagtgttt ccactgatga ttgtcgataa
    16501 ttgtttccga ttcaggttct gcgtcagtaa gttcgggtgc cttaatgccc tgacccgctg
    16561 tggcggtaga aatttcaatg ttacattcgt cccagagtcc tgcccggata aaacttgtca
    16621 acagccgggc acctccttcc accagtacgg aatgaatgtt cagttcgtac agtttccgaa
    16681 gtataaaagg cagattatca ttgctctcat ccggaatctg aacgtattcc aggttcggtt
    16741 tcgattcgga tgctacggct gtgaaaatga ttgttctttc tttttgattt ttcagattga
    16801 aattaccggg aatgatccct tttctgtcaa tggcaatccg tacgggcgat ttgccgctcc
    16861 agtaacgcac tgtcaacgat ggattatcga gtaaaacggt atttgtacta accatgatgg
    16921 ccatattctc acttcgcatt ttgtgagtga gttgctgtgt aatcggattt gaaattttca
    16981 atgcctcttc cgaatggctt tttctgattg tatcaatgaa tccatcgctg gtttgagccc
    17041 atttaagcaa tatgtacggt ctttttttct cctgaaaaca aataaaccgc cggttgagtt
    17101 tttcagattc tttcctcagt atgcccactt ccacttctat gccggcatct ttcagaattt
    17161 tcaccccccg tcccgaaacc ttcggattcg gatccgggca gccgattaca acccgtttga
    17221 tcttttccga cactatcagg ttcgcgcaag gcggtgtttt cccgtagtgc gaacatggct
    17281 ccaggttgac atacagcgta gcttccggca gcaaatgctt ctctctgacc gatgcaatgg
    17341 cctccggctc ggcatgatga ccgccaaagc gatgatgata accttcgccg ataatcctgt
    17401 cgtcatatac aaccacagcg cctaccatgg ggttgggcga aacgctaccg catccgagct
    17461 ttgcgaggtg caggcatcgt gccatgtatt tctcatcttt cggaatcatg ataacaaaat
    17521 tacagaatat ttaattactt tcgttgaaaa aaaattgttt tttcttactc tgttcggtat
    17581 tttttacttc gtgtatcatt ttaatgaact ttcaggtgcg atatttgtat taatagtgaa
    17641 ttggataaaa tgtaatcaat aaaaaataaa accatgaaaa aactgatcct tattacgctt
    17701 gcatcgctgg cactctcgct cagctcctgc aacgaactgc tgcaaatagc caatacaatg
    17761 ggaaatccgg atattccggt ttcagaagcc gaaaatgctg ccgggcttaa atcttcgctc
    17821 gatgtgggca ttgagaatgc cgtgaacatt cttggcatcg aaaatggatt tatgaacgac
    17881 gttttggtaa aaattctgct gcctgccgaa gctaaaccca ttatcgacaa catcaggttg
    17941 attccaggcg gcgaagagct ggtcaaccgt gccatccttt ctctcaaccg tacggccgaa
    18001 gatgctgtaa aagaagccac gcccattttc aaacatgcta tcacctccat cactttcaaa
    18061 gatgccgctg gaatactctt cggcggcgat tacgcagcca ccagctacct gaaggaaaaa
    18121 acttcggctg acttgcttgc agcattcgca ccgaaagtga aaaactccct cggaaaacct
    18181 ctggttgcca atttatcgac cacccaatcg tggaatgcac tcacctctgc tttcaataag
    18241 gttgccgcaa gccccgtggg catttatggt ggaatgaaac ccgttaacgt caatctggag
    18301 gaatacgtga ctcagaaagc actggatgcg ttgtttctga aagtgggaga ggaagaaaag
    18361 aaaatccggg aaaatcctgt agcacgcatc acagccattt tgaaacgggt tttcggacaa
    18421 ttggacaaaa aataattttc agtaataaat tcaaaaaaag agtataccca gggtgctctt
    18481 ttttaatgaa ttaatacaaa tcaatatgag attactatat tttgtttttg cactgatgct
    18541 ttctgtttca ggtggttttt tgttttcgca gtcggggtta gtgccgctcg accggcattt
    18601 gagcaccatt aattatccct ttgaagttca tttgtttgaa ttcaatacac agggccagac
    18661 actcaccatg gcatacatgg atgtaatgcc cgataattac aacggaaaga acatcctgtt
    18721 gcttcacggt aagaatttca acggtgctta ctggcgcacc accattcagc aacttaccgc
    18781 aaacgggtat cgggtggttg tgcccgatca gattggattt ggaaaatcat cgaaaccaac
    18841 tacttatcaa tacagctttc atcagctggc ttccaataca aaacagctgc tggatacgct
    18901 cggaatacag catacggtag tgctcggtca ttccatgggc ggaatgctgg ctacacggtt
    18961 tgcactgatg tatccggata tgaccttgaa attgattttg gagaatccta tcgggttgga
    19021 agattacaag accctcacca cgtggcaatc gctgaacgat gcatatagtg ccgaattaaa
    19081 aaacacgtac gaaagttacc ggaaatatca gttgaccttt tattacgata ataaatggaa
    19141 acccgagtac gacgaatggc tcaatatgct tgccggatgg acactgcatc cggattatcc
    19201 ggtcattgcc cggactgccg cactcaccac cgacatgata atgacacaac ccgtggtgta
    19261 tgaattcggg gatattaaag tacccacctt gctcatcatt ggtacgcgcg acagaactgc
    19321 aataggaaaa gaccgcgctc cgaaacatgt gcaacccaac atgggacttt acaacaaact
    19381 gggcagaaaa acccaacgac tcattcctga atcaaaatta gtggaactga aaggagtagg
    19441 ccacatgcct catattgaaa ctttcgattt atttataaca tcggttttga attttctgaa
    19501 tgaaaaatag cctggataaa cccgaaacaa tcaccgaata catagctcaa tttcccccgg
    19561 atattcagga cagacttaca gctatccgaa agttgattca tgaactggtg ccggaagctg
    19621 tagaagtaat cagttacgaa atggcggctt tgaaataccg gaaaaagatc ctggtgtatt
    19681 ttgccgcttt caaaaatcac atcggttact atgccactcc cgataccaat tctgcattcg
    19741 gggaagaact tttgcactat aaagtcggca aaggatccat tcagtttccg cataacgagc
    19801 ctcttccact ggagctgata gcccgactta ccttgtttaa ggcaaatctg attgatgtga
    19861 aagtaaatac agaaaaaacg ttgactaaat ccaattcctt aaaaaatatt gattccaatc
    19921 cttgaaagca tgccaatgta ttttaggtaa gtatgcgaat aatcataaat aaaatatccc
    19981 gatgaaaaat caccgggata tttttgtgaa tgaattgttt attccgacaa aactgtctca
    20041 gaacgaaaat gcagcaccta tcatcgcatt tattccctgc acatcgtatc cgctgtaggt
    20101 ttcgtatttt ttgttcagga tgttgttcac tttgaggaaa accgacatcc agtcgcggta
    20161 tgcgtaagcg gcacccagat tgagatcaag tacgggagcc attcgtgtgc cgtcggtttc
    20221 ggagagcagg gcgtatctgc cttcctgaaa aataaattgc gtatttacgc tgatgtcgtt
    20281 attgattttt acactggctc ccaggtcggc atcccaggcg ggcaactgcc aggcatgtgc
    20341 ctgtccgtcc acattccagt aatggtaagc acccttcaga taaacattta cctgattttt
    20401 gaagtcccat gcagtgcgca tacccacggt agcccgttgc gcttttgagt agattacatc
    20461 aaaacggttg tggaaaaaat tgttcatgtt gccgggcatc tggcttgccg aaggtacaag
    20521 cagttcataa ggcctgttga tgtagaaata ctggttgttg atgattttat actgtccgta
    20581 tacatcgatc aggagattga atacaggact gaatttcagg cctgcatacg catctatggg
    20641 tgtataggtg tcttccggtc ggttttgggg cgacaggtac ctgttttcgt tgtaaatgcg
    20701 gttaagtgaa ttcacggtca gatcgcccgt tacgccgccg tagagtgcca ggtattcggg
    20761 cagggcattc cattgcgctg tcacgtcagg tgaaggggtg aaaacctgtc cgtaccctat
    20821 cgatatgcct gtcttaactc ccaagcgaag gtaaccgcgc tctccggtaa gcatccagta
    20881 gggattgaac ttcagtaggg agtactgttc gtcgaaattc aaacccgaag ctgaattttt
    20941 gttgtacata aaactgttga tatccacatc cattccgagc ctgttctttt ggaaaggcac
    21001 actgaatgag ccattaatcc gtatccggtt ctccgtgagg ttgtttccgg cagatttgaa
    21061 taaatcgtaa tttactcccg catcaaaaat cagatcgtcg gcaagcggta gcgaccgggc
    21121 gcccacgtgg gagttgaagc gccagtgggt atcgttcagt ggcaatgccg agagttgaaa
    21181 cagcgataat gaattgtctt cgggcgtaga gtaggaggca tcgccgtatc tggatgccac
    21241 atccgacatg atgaccgggt cagtccctgc aaaactgcgg gcgtagtaat tgaagaagtc
    21301 gtgcctgcct cctgcaccgg catacagttc cagcttgtca aataaatggt tgaactgcag
    21361 cgatccggaa gttttggagt gaaccttatc accgaaagtg cccaggtgat gcaaacctac
    21421 atccagcctg ttctggtcgt ttttcagaag cgggtacatg aaatcgccca gcgtgttgag
    21481 cggatatccg aatccggctc tgaaaaatcc gtttttggca ggcagcggtt tgtgaaccag
    21541 ttcttcgggc ttgagcaggt gaatgttgta gtcgatgttg agcggagttg taatatccga
    21601 atataccggt tttgtttttt ccaccgaggg ctcaatcact gtagggaatg tgatgatttt
    21661 acctgcatcg attatcacag gctgaaattc acgggtaact gttacacttt tatgaaccac
    21721 cgtgtcgttt tcctgagcag caaccggcaa tgcagaaata aaaaccggta acgacaggaa
    21781 ataaaatatc ttcttattca ttttatgtgg attgtgtgac caatagattt tatactatca
    21841 gaacatttta taattacgtt ttttttaaaa atcaataact caatgtgccc tcttaccaaa
    21901 atcggtaaaa ttccaaaaaa aatctcctta aagtttgcca ccctcagtcc ggaggttcag
    21961 gcaggtttat tttattaccc tttcctttgc ccgtttttca attccggcca ttctcacggc
    22021 aatcatttcc tgcacatcgt ccttcgcggt atagtttttc tgcagactct gcagatactg
    22081 ttttgcctga aaatcatcgc ctttctgtgt ataaatatcc gaaagtatga taaagctgcg
    22141 agcgagccag tactgatggg gtgtactttt ctttgcaaag tcaagtactt ccttttcgga
    22201 gtcgttcagt ttattcagtt tgaagtaagt ttccgacagg gcaaatttgg cttcggcgcc
    22261 taatgaggtg cgtgtatcaa tttttatttc tttcaagtct ttgagtgcct cgttgaattt
    22321 gttttgcctg gaaagggctt tggcgcggtt gagcagtgcc tctgtacgca tctgagctgt
    22381 tgattttgaa tcggcaatga tttcgccggc tatctgaatc gtttcatccg tgttattaag
    22441 gaagaagctc gtacggagca caccaagccg ggcaatgttt ttgttttcgg tagtttgtgc
    22501 cacctgttcc agccgtttga agtaagccat tgaagctgtg taatccttgt tgtcgaatgc
    22561 tattccggca gcacgaaggg cggcttcttc ggtgtaagga tttccgcgtg aatcaagtat
    22621 ctttatgtat tcatccaggg cttttagttt atcacccaag gtgtagtaac tgtcagccag
    22681 gtagtactga gctatggtgc agaattttcc tcccggacaa tacctgctca ggtacgagtg
    22741 aagaccggca gcagcttcgg catgattgcc ggccatatat tgtttctcgg cagctacaaa
    22801 gaggatggag tctgcccttg ccatgctgcc gctttcgatg tttttaccta tcgatttggt
    22861 gtaatccagg tagtcgccca ccttattggt gttgatgtag atggtttcaa gactttcaag
    22921 tgccgtatcg gcttcttcgc tggccggata ttccgccacc acttttttga aggcgggaat
    22981 tgcccgttcg aattcgtgct ggttgaaata caccataccg gtttccagca gagctttcgg
    23041 agcgagttgg ctatttgggt agccggctat caggcggtta tatgtttcta ttgtttttgc
    23101 attgttttcg agcatcaggt atgcccgcgc tatttcatac agggcatcat cgccgtattc
    23161 cgaattcggg tatttgttga gcagatcctc cagtttcgaa attttctgat tgtagttttt
    23221 attcagtccc gtcatgtaag ccgactgaaa cagcgcataa tcgccgttgt aggtagtgtt
    23281 catcactttt tcgtagtatt cggaggcttt ggtaaagttg cggttgctga agtaggcatc
    23341 gccaatgcgg ttcagagcat cggcataagt cggtgtggag ctgttttttt cttcattcac
    23401 gtatttcaga aacgatgaga gcgattcgtt gtatttcttc tggctgaacc aggcatatcc
    23461 aagcgcatag tgtgcctgcg tatagtttgg attactcatt acattgggct gggagaagaa
    23521 tgagtttaag tcggataccg acctcacata atcgcggctg cggtaatggc tttcgccccg
    23581 ccagaaatag ctgtcggcca ggtatctgcc agtgggagcc gactggatgg ctttggtgaa
    23641 aaactcaatg gcccgctggt agttactttc ggcaaacgac tcggtaccaa gctgatattc
    23701 caggtattgt ttcgtttcgc ggagtttcgg ggtcagtgtt tttatgtttg ataccgactg
    23761 ataagcggcg ctgtaatttt ttgaagtcag gtacaccgtg gataaataat cgtaggcttt
    23821 gtcgatattg gcagaatttg gaaattcccg gatgaatagt tcgaatgctt tgaccgattc
    23881 gccgaaagcc gtattagttt cgtaggtggt gagagcgtag ttgaaaaggg cttcttccct
    23941 cactttagta ttgaaattgg tttgcgtggc agcttcgaat gccagccggg cgttttcctt
    24001 attgcccatc gccacgtatg cattgccgag ctgaaggtaa gcgctttcgg tcatttcatc
    24061 gggctctatg gttacctgcg agagatactt cacggcatcc gccggtttac cggttttcag
    24121 gtagctgatg ccgaggtagt acatgtcgtt tcggagcacc tgtgggaaca ggttttcgta
    24181 ttttttcagg ttgtcgatgg ctttggtgta gttgccgtcg gcgtatgctt tttcgccggt
    24241 cattctgtaa atttcggcat tgttgatatt gtccggatat ttacgaatca gcatctcagc
    24301 ccttttttcc atttcgccga aattcctttt gaaatagtat atctgggcaa tgtagtaagg
    24361 agcaatgtct tcaaattcgg gatttttctc tacggcaagc aggtcggtga gggcctcgtc
    24421 gtagttgcca aggcggtatt ggctgtagcc cgtataatag cgggcagcag gctgataggc
    24481 agaattcatg cttttcagcg ttttgaaaat atcgagggct tttggaatgt cgtttatttc
    24541 caggttggca tatccgcgac tgaagaggta atcggttctg tcgcgctcca cgaggtgcgt
    24601 ttcatctatc tgcctgaagt atgcaagcgc tttgtcgtag ttgcggcttt catattcgag
    24661 catgccgagc atcagctgga tgcggtccag gtaaggggtg tagggatgat tgtccaggta
    24721 ctgcagcagc aagtcgtacg catttttctg gcggagttcg taggcacccg atgccatgta
    24781 aaaagctgct tcctgaatca tgccggcgtt ggtagtctcc gtatttttca gaaattcctc
    24841 gaaattgcgg tacgatgcag cccactttcg ctggtcgaaa agttcttttc cttcgtggaa
    24901 aagattatcc cgttgggtaa aaaccatcga atgctgtgct cccgctactt gtataagcaa
    24961 cgcaaaaaca gcagcaagga ttattttttt agacataatt tattttgttt gtagtttgga
    25021 atattgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg aaaagccggg
    25141 gatttatttc tctaacgggt agaaaaaaac attcttccac atcatcggca gcatggattt
    25201 ccacgttatt ggctactttt ccttcgaaaa acatatccag tgtaggaacg ttcagtccgg
    25261 aatataaata atcgttcggc agcgaaaaaa cgtaccggag attatccatc cggataccca
    25321 gctcttcgtt cacctcgcgg gcaatgccct cttcggctgt ttcgtcgtaa tcaatgaatc
    25381 cgcccggcaa atcgagtgtt cctttagccg gttcgtgtga gcggcggcac accagcaaat
    25441 cgccgtcttc atttcgaatg aaagccgctg tggctgccga gggattttga tagtagataa
    25501 acccgcaaat atcgcaccgt tttgatttta cgttgtgaat gatatactta tcggaaccgc
    25561 aggcaggaca aaatttgaat ttttctgaga atttcattaa taagttgtat tttaactaca
    25621 aagataacta ttaataaaaa agcagacaaa cagaatttcc ggagaatatt atattttacc
    25681 atcgtttaac ggttgttatg gataagtaaa atgaaaagcc ccctccaact cctcctcaga
    25741 cggagttttt gggcagaaag gcatttgtta ggttttgaaa aaaaaacacc gagcttgttg
    25801 ataccggtgt ttctcgttac atcaggcatc cgctgtacgc ctcgcttatt tgtttcagct
    25861 gggatattct attgaaacag gtcactccta cgctactgat tatcacactg ttttgtaccc
    25921 tgatgtttta tctcacttcc cgctacgacg aaaaagaatg tattgcttat tttggcgaaa
    25981 aatacaccgg ttacatgaaa cgcacccgga tgttcattcc ctttgttttt taaatttcgc
    26041 aaaataaccg ctatctttgc aggcttaaaa aaatacgatt ttatggtttc agtggacggt
    26101 ttggcggtag agtttggcgg cacaacgctt ttctcggatg tttcatttgt gataaatccc
    26161 aaggacagga ttgcgctgat gggtaaaaat ggtgcaggca aatccacgct gctgaaaatt
    26221 ctggcaggag ttcgcgatcc ttcgcgcgga aaagtctcca tgccccgtga ttttaacatc
    26281 gcttatctgc cgcagcacat caaaaccaac gacagccgaa cagtttttga agaaacggcg
    26341 caggcttttg cgcaattgca cgaactggaa agccgaatcg cttacctcaa cgaacaactg
    26401 accatacgta gcgattatga atcggatgaa tacatgcagt taatagagga agttacagca
    26461 ctgagtgaaa aattttacag catcgaggaa accaatattg acgagcacat cgagaaaacc
    26521 ctgcttggtc tgggtttcga aagagaagat ttcgaccgga atacggcaga attcagcggc
    26581 ggctggcgta tgcgtatcga actggcaaaa atcctgctgc agaatccgga tgtgatcctg
    26641 ctcgatgaac ccaccaatca cctcgatatc gattcggtac agtggtttga ggattttctg
    26701 attaatacgg caaaagctgt ggtacttatt tcgcacgacc gtgcatttgt tgacactgtt
    26761 accacccgca ctattgaggt tacgatggga cgtatatacg attataaagc tacctacacc
    26821 cactacctgg aactgcgcaa ggagcgtcgt gagcaacagc aaaagcagta cgacgaacag
    26881 cagaaaatga tagccgaaac gcaggagttt attgagcgtt tcaaaggtac gtattccaaa
    26941 acccttcagg tgcagtcgcg ggtgaaaatg ctcgagaagc tgcaaattat cgaagtggac
    27001 gaacaggata cctccgcgct gcgattaaaa tttccaccat cgccgcggtc gggaaattat
    27061 ccggtgatag ccgaaggggt tggaaaagca tacggagaaa aacgggtctt tgcaaacgct
    27121 gattatacca tcgaacgcgg acagaaagtg gctttcgtcg gtcgcaacgg cgaaggaaaa
    27181 tccaccatgg tcaaatgcat catgggcgaa acagaacatg aaggaaaact gacactcgga
    27241 cataacgtga aaatcggcta ctttgcccaa aatcaggcat cgctgctcga cgaggatctg
    27301 accgtgtttc agaccatcga cgacattgca gtgggcgata tccgcacaaa aatacgggat
    27361 attctcggtg catttatgtt tggccgcgat gacgtggata aaaaagtgaa aattctttcg
    27421 ggaggagaac gaacccgcct ggcaatgata aaactgctgc ttgaaccggt taacctgctt
    27481 atcctcgacg aaccgaccaa tcacctggat atgcgcacaa aagatatttt gaaacaggcg
    27541 ctgactgact ttgacggtac gcttatagtg gtttcgcacg accgcgattt tctggacgga
    27601 cttgtgacta aggtgtacga attcggacac ggcaaagtaa ccgaacacct cgaagacatt
    27661 tacggattcc tggcgaagaa gaaaatggaa agcctgcgtg aaatcgaaag caaatcgaaa
    27721 aagtaaaact ttctttcacc cgaaatgtaa tacattgtat tcctgaaata aaaatcaggt
    27781 ctgcgcccgt ttcacctgta taaccaaaaa gaaagattta tgcaggctca ccggacgggc
    27841 tcatccgtaa tcaaaacttt ctacatttgt ccgtttttta tctataacgg cggatttggg
    27901 atggttacat tgctttcgga agttgagtgg taaaaaaaaa cggttctgta aatgcaaatt
    27961 gtattacaga accgtttaca aaacaaaaaa attaaggttt acggttttat atgtatgatg
    28021 gcattaattc atgaccggca tctcggagat ggtaatgtct tccggaattt caagtttact
    28081 gtccggaatt gatatgcctt cttcaaattt tacagcttcc atcacatacg catcggatgt
    28141 cattttcaga gggattcctt tgtagtacca cattttcatg gtagtgcctt cacttgtcat
    28201 ttcaatgacg atgcagtttt tgcccaggaa gtcttcgttg ccgataattt ttcccccttc
    28261 tttttcaatc atctggcgga aagtttcgtt gtcgtccggt ttgatattat ccatagggtc
    28321 ttcttcattc ccgtccgact tcatttttaa tccggttttg tttgtcaaac tgattacgta
    28381 agtataaccg tctttaccaa ttgttaatgc actttctttg gttttttgtc ccatcatctc
    28441 gttttcagtt tcggtttcca ttaccagacg gtcgttttta atgtcaatcc actgtttcga
    28501 aactgaggta ctcttcattc cctgcatatc cattgtagtt ttatattcta tataatagga
    28561 ctttgtatca gctatttcct gagctatttc ctttgcagtg gcgtcgggat ttgaactgtt
    28621 tttgtccgct ttttgtccgc atgatagtag catggcggca attgttccta aaataaaaag
    28681 gcgtgttctc atttgattga atttttttat ggattaatat cttatgaaag aattctaagt
    28741 taactatttc cagaccggaa aaaacattat tttgagtttt gcactgtatg aaggaacttc
    28801 ttcgtttgtt tcagtagctg ttactgtaaa tggttttctt tttttgatgg tttgaacctt
    28861 gtcggcaatg ggaatattaa attgctgccc gtgattgtca aatcccatgt aacaattgat
    28921 gcgcagatcg gccgggttgt gcgtatctga actgcaaagt atcaggtcgg cgtccaccat
    28981 ttcagttgcg aaggtgctgt tggcctgtat aagctgcagc cgcggattga atttacctcc
    29041 cttgtgtgaa acaacaccgc cgtcgcaggc ataaggaaat gtgtaacggt cgtcgttcac
    29101 gatggtcata cagttgtctt caagttctga catgtttaca attatataga aattatccca
    29161 g
//
LOCUS       DAORKP010000007        28446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_41_length_28446_cov_9.951182, whole genome shotgun sequence.
ACCESSION   DAORKP010000007 DAORKP010000000
VERSION     DAORKP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28446
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_28446_cov_9.951182"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLN75_00930"
     CDS             <1..972
                     /locus_tag="PLN75_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRG02710.1"
                     /translation="KQAYESPKDTVKFDCHSYLNHKDAVWTWSFSPAPKYVSNIHARN
                     PRVVFKEPGMYSATLSITQNGQTVSKTVTDMVEVRVAPSIDNCDRPGELPKNIMKATV
                     DSYQSGEDGSKAIDGNTSTLWHTPWSGSPGHPHYIQLDLGDNYNISKLIHTPRTDGSN
                     GRIKDYELYISDNVSNWGTYVLKGTFENTSSPTTVEFPVKTGRYVKLVALSEVNDNNW
                     TSIAEIGFIGCRVTTSVNDYFSDATLHAFPIPATNLITVNLPFQNGLNAYSYNVFNTS
                     GQLLKSGNAGENQTSISIDVNSYPSGQYVVILEDKTGIHYRVKFIKR"
     gene            1134..1784
                     /gene="rpe"
                     /locus_tag="PLN75_00935"
     CDS             1134..1784
                     /gene="rpe"
                     /locus_tag="PLN75_00935"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRG02711.1"
                     /translation="MIKIISPSLLAADFGNLQADCEMINRSEAEWFHIDVMDGVFVPN
                     ISFGFPVIEAVKKHCRKPLDVHLMIVEPEKYVERFVKAGAEILTVHLETLKNPFDTLR
                     SIRELGVKVGITINPDVEVRNLYGLVSQVDMVLLMSVFAGYGGQKFIEETYPRIDELK
                     DIIADENPDCLIQIDGGVNLENAPKLFDAGVNVLVAGSSVFKAPDPVSYIHQLLHQ"
     gene            1801..3861
                     /locus_tag="PLN75_00940"
     CDS             1801..3861
                     /locus_tag="PLN75_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRG02712.1"
                     /translation="MNFLQRTPFFRFLLFLAAGIVLFQFVRLPAVAIAGIASVAGIGL
                     ITDFFLIKKDIHYRFRWIFGFATFLILLAAGYTSSLIFEKQHVFNELNDRKIYEVELR
                     SAPVEKERSFMSKVRLIRKYESGKEKVCSGNAIIYLQKDSAASELIIGDRLLIEAEFQ
                     RPDGVQNPGGFDYANYLKRQGINATAYIPAEKWRKNGENIHFNLLRLADRSRNLLLNI
                     YRKFGINGDEFAVLAALTLGYTDALQPDLLKSYSATGATHILSVSGLHVGIVYAVIFF
                     LLGFLSKTRSGRIIRAVIVSLFIWMYAFLTGLSPAVMRAALMITFVAAGTCSDKKSQT
                     VNTVLISAFLLLLLNPNLLFNIGFQLSYSAVLSIVFFQPKISKLLFVKNSFLKKVWDL
                     TAVSIAAQIGTAPFALYYFQQFPNYFLLTNYVAIPISTVIIYLAMALLFLSKVPLLSS
                     AIAYALKWTTWFMNFLIEKIQDIPGSICIIPLNVIQLILCFVVIFSFSAYFINRKFAP
                     LMAGLASILLILGIYNYQKYSSLTSSKLIVFSDNRVPVINFTDGTDNYVYTTDSVQAV
                     KTASSYWKSNLLSKPTYLNDNEWFKDGFVSFGNMRILILKDDFLKFKVAPKPLDVDYL
                     IISNKLKPKMEQILNCIHPEHVIVDKSISAWYANHIREVCLARQINYYSVAEKGAFIS
                     HLER"
     gene            3944..4984
                     /locus_tag="PLN75_00945"
     CDS             3944..4984
                     /locus_tag="PLN75_00945"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRG02713.1"
                     /translation="MISKVIAEELIHKLKREIENAENIVLVTHVGPDGDALGSALGMW
                     HFLMTIDKTPTVIVPSPPPNFLMWMPGADRMIIYSEKKEEADKAIDEAELILAVDFNT
                     PNRLALMAEKVVNAKAHKILIDHHLNPSPDFANVVISYPQISSTSELIFRAICRMGHF
                     SDINLGCAECIYVGMMTDTGGFTYNSNHEEIYSIIYELIKLGVDKDDIYRKVYNTFSA
                     DRMRLMGYCLYKKMKIYPEYQAALITLTQKELHEFNYDNGDAEGFVNIPLSIEGINFT
                     VFMREDPDKIKVSLRSQGTFPTNKFASEVFGGGGHLNASGGESFTTLQAAVRKFEEAL
                     PLYREFLDTPNP"
     gene            5016..6308
                     /locus_tag="PLN75_00950"
     CDS             5016..6308
                     /locus_tag="PLN75_00950"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRG02714.1"
                     /translation="MKNNINNSFEKEYPDNSSLNFPFREVRRNKYIIILGDGMADEPI
                     ASIGNKTPLQAANTPNIDKLAKMGRCGMLDTIPRGFAPGSEIANLSVLGYDLNECFEG
                     RGTLEAASMGVEIYDDEMAMRCNLICVEDGKIKSHSAGHISNEEARELILFLRKELGN
                     ERVSFHPGVSYRHLLKLKNGNKNLHCTPPHDVPGRDFSPLLIKPEVPEAQETAGFLNK
                     LILKSQELLENHPVNLKRAAEGKEKANSIWPWSPGYKPTMKKMTDIFPINSGSVISAV
                     DLIKGIGVYAGLKNIDVAGATGLYNTNYEGKARAAIDALKTDDFVYLHIEASDEAGHE
                     GDVNLKIKTIEYLDNRIVKPIMDELLTWEEPVTIALLPDHPTPCVMRTHTNTPVPFII
                     YRTGNTPDETEIYDEFACKNGSYGLLKGNEFIREIFRQ"
     gene            6388..7512
                     /locus_tag="PLN75_00955"
     CDS             6388..7512
                     /locus_tag="PLN75_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRG02715.1"
                     /translation="MTIIPNIEVTDCPKRHSEVFRTEGIKYTGSKLKILPDIMQLIHQ
                     LPDVRTVIDGFSGSTRVSQALARTGYDTTSNDISVWSEVLATCYLKSTKPDSYYREIT
                     DYLNALPGSDGWFTEHYGTAEEGQKKPFQLKNTRKLDSIRDEIDRLELDWEDKCVILT
                     SLIYALDKIDNTLGHYAAYLNNWSKRSFNDLTLKLPQRFRCTGENSVVRSDIFDTIEK
                     NKFDLAYFDPPYGSANNKMPPSRVRYASYYHIWTTVILNDKPTLFGKANRRTDTRDGV
                     SGSVFEEFRTNENGRYLAVEALEKLIRETKAHYILLSYSSGGRATKSDLTDIILSSGR
                     LLKVIEIDYTKNVMSEMRWTNEWVNSNGKHKEYLLLTEKV"
     gene            7567..8883
                     /gene="thrC"
                     /locus_tag="PLN75_00960"
     CDS             7567..8883
                     /gene="thrC"
                     /locus_tag="PLN75_00960"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRG02716.1"
                     /translation="MKYYSTNKKTPNVTLEVAVIKGLAEDKGLFMPERIEKLPDEFFE
                     TIGTLSFQEIAFAVAQAFFGEDVETAALKKIVYETLPFDTPLVEVEPNIFSLELFHGP
                     TLAFKDVGARFMSRMLAYYISRQSNADAVNVLVATSGDTGSAVANGFLGVEGIRVFVL
                     YPKGKVSKIQESQFTTLGQNITALEVDGTFDDCQRLVKSAFLDEELNRKMKLTSANSI
                     NVARFLPQAFYYFYAFSQLRSMHPKQTAEELGTVFCVPSGNFGNLTAGLVAKRMGLPV
                     KRFIAANNRNDIFLQYLKTGIYSPKPSVATIANAMDVGDPSNFVRILDLYGHSHEAIS
                     KEISGVSYTDEQITETMRNCFVETGYLLDPHGACGYQALKDELGENENGIFLETAHPA
                     KFSETVMSIQGLNVEIPARLLEFMKNKKHSIPIGTDFEEFKTFLLK"
     gene            complement(8947..9882)
                     /locus_tag="PLN75_00965"
     CDS             complement(8947..9882)
                     /locus_tag="PLN75_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRG02717.1"
                     /translation="MKKIALFLGLYLIFGMVASSQITDAESKLKKVETDTVASWKKGG
                     FSGLNFSQTSLTNWAAGGQNSLALNGLFNFFAVYKDTLNVWENTLDLGYGFLRQSSYD
                     GIMKTDDRIEFNSKYGRKAFKNFYYAGLLNFRTQFSPGYNYPNDVDKISNFMAPGYLL
                     AALGLNYIPNTYFNAFLSPLTGRLTFVLDEALSQKGAFGVAKGEKMKKELGGYVRLAF
                     AKSDFKQGFFQNMSIGTKVDLFSNYLKDPQYVDVNWETLLGMKVNKYITVSLNTQLIY
                     DHDVRIDYNQSGSIDDDSPRVQFKEILGIGFMYKF"
     gene            complement(10011..10643)
                     /locus_tag="PLN75_00970"
     CDS             complement(10011..10643)
                     /locus_tag="PLN75_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02718.1"
                     /translation="MPQNDRNKTKADEAQVKNLTLSEKNAREKQALLEKKEKSKNILE
                     RARRIHAARKAHEARKAKEERDARMERRAAERKKAEEARKLKNMKNSAPGNSKTESKA
                     MPEPQYVDFAITARKYKTQLTKKYMQRKFWVNPSPYEIQSYIPGTIISIDVKEGKIVE
                     EGTRLLILEAMKMQNRVDMPFTARIKKINVKAGERVPKDFIMIELEPVES"
     gene            complement(10700..12244)
                     /locus_tag="PLN75_00975"
     CDS             complement(10700..12244)
                     /locus_tag="PLN75_00975"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRG02719.1"
                     /translation="MSSKKNIIALRQKKAIVEKGGGDKAIEKQIAMGKMPARVRILAL
                     LDEGSFHEYDMFVEHEEKNFGMETKELPGDGVITGTGTIGGAPVCIYAQDFTVMGGSL
                     GLKHARKITKIMDHALKMKIPCIGINDSGGARIQEGIGALAGYGEIFYRNTMASGVIP
                     QLSVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYAANEMECFKQIKTLISLIPHNNQKRAQSIEPKDPKFRKKIENIIPAD
                     PKQPYDVREVILALVDESKFFEVQELWASNIVIGFGRMDGETIGFVANQPMYLAGVLD
                     CDASDKAARFIRFCDAFNIPIITLEDMPGYLPGVDQEHAGVIRHGAKMLYAYSEATVP
                     KVTVILRKAYGGGYIAMNSRHLGADFVYAWPSAEIAVMGPEGAANIIFRKEIMEAEDE
                     DAMRQQKVKEYVEKFANPFVAASKGYIDAVIEPDETREMLLHSLSVSRDKDDSRPTKK
                     HGIPPF"
     gene            complement(12302..12892)
                     /locus_tag="PLN75_00980"
     CDS             complement(12302..12892)
                     /locus_tag="PLN75_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02720.1"
                     /translation="MSEDSNFIRTVLSGNMGKKEVEMIRSLLLHDAEQLEYVFSLIFD
                     TDEKIAWHAAWVIEKVSEHDANLISEDNNRQLVGFVLRNKHQGLQRLCLSILFNLPVC
                     LPVSVELINLCFDQMLSTRQSVSVQVLSMKYLNNICMIEKDFVPELKVTLENADNAMY
                     SAGYNAAKRHILKSLAGADRSGHLPDVSLRHYLFEK"
     gene            complement(12905..13195)
                     /locus_tag="PLN75_00985"
     CDS             complement(12905..13195)
                     /locus_tag="PLN75_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRG02721.1"
                     /translation="MRRKNTEKLNEILAQVLKQNHLDEKLYETRVIQSWSAVLGDNIM
                     RYTTAIYFNKKKLFVTLSSSVLRHELFLTRDEIKNSLNKYVGADVVKEIVFQ"
     gene            complement(13235..14164)
                     /locus_tag="PLN75_00990"
     CDS             complement(13235..14164)
                     /locus_tag="PLN75_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRG02722.1"
                     /translation="MRKEIVLAVICMLGLLPANAQTMHQAYLEYIGKYSEIAVEQQNL
                     HKIPASITLAQGLLESAAGKGKLVSISNNHFGIKCSDWTGQKVYADDDKRNECFRKYN
                     SARESFEDHSQFLITRPRYASLFGLDATDYKGWAHGLKAAGYATDERYAYKLIKLIED
                     YDLHKYDTGSKPDMKKADSESNRETYTWGKASVTTLKGHNLFRNNGVKCVFAEAGDTY
                     AGIAHEFNLKENKILNYNDLNQSHELTPGTVVYLSAKRKKASAEYQTHQIKPGENMYR
                     IAQKYGIKLQSLYDLNQMPYDQGAVVNKVLKLR"
     gene            14279..14758
                     /gene="cdd"
                     /locus_tag="PLN75_00995"
     CDS             14279..14758
                     /gene="cdd"
                     /locus_tag="PLN75_00995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRG02723.1"
                     /translation="MQYLKYETRITEYLPNEIPARYQSLIAEAKNQTEKAYAIYSQFR
                     VGAALLLENGMTVTGNNQENSAYPSGLCAERTAIFYANSQYPDSAVKMIAIAAFAGGE
                     FLKSPITPCGACRQVLLETETRFGKDIEIILYGTEKTYVLENVKQMLPLSFNGDSLK"
     gene            14825..15208
                     /locus_tag="PLN75_01000"
     CDS             14825..15208
                     /locus_tag="PLN75_01000"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRG02724.1"
                     /translation="MKKEMFTIEYPLENSSENLLWTMIGDPLGLALWFSEGVTVDKNE
                     YTFSWDDHYQTAILQEVREGKNIRFQWEEDKDTDAYFQLEIITQDLSGHIGLLVTDFA
                     AAKDKDDAIMLWNKQIDDLRRKAGM"
     gene            complement(15348..16409)
                     /gene="mnmA"
                     /locus_tag="PLN75_01005"
     CDS             complement(15348..16409)
                     /gene="mnmA"
                     /locus_tag="PLN75_01005"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRG02725.1"
                     /translation="MNIAALISGGVDSSVVVHLLKEQGITPALFYIKIGQDDDELLHC
                     SSEEDIEMATLIARKYGCKLHVVDLHKDYWDTVVAYTIDKVKKGLTPNPDVMCNKLIK
                     FGVFEERYGNDFDKTATGHYATTIEENGKTFLGTAKDPVKDQTDFLAQIDSLQVSKLM
                     FPVGHLMKNEVRKIAEKAGLPSAKRQDSQGICFLGKVNYNDFIRRYLGEKPGPVVEFE
                     TGKILGTHRGYWFHTVGQRKGLGLSGGPWYVIKKDVPGNIVYASRGYDVETQYGREFC
                     MNDFHFITENPWNDIEKGVDITFKIRHTPEFTKGVIMKTHTGYRVFSAEKLQGIASGQ
                     FGVVYDAASRICIGSGEIA"
     gene            complement(16473..17129)
                     /locus_tag="PLN75_01010"
     CDS             complement(16473..17129)
                     /locus_tag="PLN75_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRG02726.1"
                     /translation="MEVSRIILADNQYITREGARNVLSRMGHFEFKDVLSRKELLAEL
                     SKDVFIVVVLDYSLFDFRSVDELFNIGIAYPNVHWLLFSSELSDEFIRRAYFNSPDFS
                     ILYKDSSADEIRTAFLQVIRKSRYVCSVASNVLLSNVYKQKTEAGRTHLTATELEILK
                     EIASGKTTKEIAEIRHSSMHTIISHRKNIFRKIEVNNVYEATKYAVKAGLIDLSDYYI
                     "
     gene            complement(17232..18134)
                     /gene="gldN"
                     /locus_tag="PLN75_01015"
     CDS             complement(17232..18134)
                     /gene="gldN"
                     /locus_tag="PLN75_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRG02727.1"
                     /translation="MKKYSLIFTVFTLLTLSGNMQAQDPNASFFDQTGNIRLQTMELD
                     ALADTIAVINHRAEDVTWSRVVYRVIDMRDKQNFQLYFPTRPNEQHRSLFRLMLDAVV
                     SGINVYSRNPRDIKPQFDDILTGEELSRVFAYDESVENNLIQVDPITEQRTINSDQYM
                     RYVRNQLKFLIQEVIFFDTHTSRLYSKIIAIAPMYALHPDNMANNDSMAYFRNSVLCW
                     FSFDELRPYLIRQLVIPNGNDSQRMTYDEFFSQNLYSSYLLGDSNMYSRMLLEFSLDE
                     NSLRAEQKRIETELMNFEQDLWEN"
     gene            complement(18189..19718)
                     /gene="gldM"
                     /locus_tag="PLN75_01020"
     CDS             complement(18189..19718)
                     /gene="gldM"
                     /locus_tag="PLN75_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRG02728.1"
                     /translation="MSGAKNCPETPRQRMIGMMYLVLTAMLALNVSSEILNGFGLVDS
                     SLRTTIASSETRNNSMYDDFEFMNKKNPAKVKEWLDQANEVKKNSDELYNFLKDFKYG
                     ILKIADGKKADKNAINIIAREDIDAAGEYAIVKGNGKQLRQKIEAYREFLKKTNPSKA
                     EMYSAMFATGGGSRWESKLFESMPVSAAITILTKYQSDIRSAEAETVQYLKSRTDFED
                     FRVNKVQAFVVPNSRYIIQGGRYSADIILAATDSTRQYEYFVNGTRIPAVGAKKFGKY
                     EFVPSQTGTFKYSGTIRMPDNQGGFISYPFTEEYTVGKPAATISNEDLNVVYRGIDNR
                     FSVSVPGIAADNVRVTVDGGSATKISGGNYIVRANQDGEITIFVAALVEGREQQMGSG
                     VFRVKYLPDPIAYLQYTDAGGVPREIKEGRVTRKQLQNARIIASYGEDALVQAKFEIT
                     SFTQLTIAGVATSSSGQLSAKQKSDLEGLEGGDIITFKNIKTVGPDGKTRGLGVIQVE
                     L"
     gene            complement(19779..>20249)
                     /locus_tag="PLN75_01025"
     CDS             complement(19779..>20249)
                     /locus_tag="PLN75_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02729.1"
                     /translation="LANVALAANGFARNIETASEMAASFADTQQKLNTTAETLAASYQ
                     GVNTEMDHVVSNTRNYSVNVDNVNRSLSTINSIYEIQLRHIQSQTESLNQQAEAIRQA
                     TTHIDGVASDMDKMKEAAAAAQAESQRYQSATRKLTRQVEDLNAIYGNMLNALS"
     assembly_gap    20250..20349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20670..22037)
                     /locus_tag="PLN75_01030"
     CDS             complement(20670..22037)
                     /locus_tag="PLN75_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRG02730.1"
                     /translation="MKKILPVLFIASLMVSCNKPMGELVGQGNKGSFRDANPYGMVYV
                     KAGSFIMGPNDQSVFGEVGEQPRKVTLNAFWMDDTEITNDEYKQFVNYVRDSVALRML
                     VMAGRDEFRKPIKGDVDEATPEEALLNWKEKIPWNSKDEEIQDILNALYYQGGDVLGG
                     KKEIDPSKLRYIYSWYDYEQAALPSNRYDINTGKFVAGAKVRRDSGYVDENGLMRNVT
                     ITKPLQTRSDFFSSRIVNVFPDTAAWVRDFQYTYNDPKMKMYFSHGAFSQYPVVGVTW
                     EQAQAFCNWRTKLYYGTNLVRGEEFRLPTEAEWEYAARGGRELALYPWGGNYVRDSKG
                     CYLANFKPARAGFGNDSGTTTMKVGSFAPNNYGLYDMAGNVAEWTSSAYNPSSGAILS
                     EVNPNYQYNSKNDDPPVLKRKVVKGGSWKDIAYYLQNGVKTYQYQNESRPYIGFRCVR
                     FYTGD"
     gene            complement(22198..23181)
                     /locus_tag="PLN75_01035"
     CDS             complement(22198..23181)
                     /locus_tag="PLN75_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRG02731.1"
                     /translation="MIIQIENTSKKCITAMLLSFVFCLPVFSQFEAQMSQYMFHSPTF
                     NPASLGENQMINVSGQHRIQWIGMPGAPQTTYFTVNAPFKSGEKVTHALGIKFLNDKI
                     GAFTNQSAHLQYAYKRKIGKGIASLGLDLGFVSVGFVYDSIKNATINSEFHDFLGDAA
                     IPAADETGMAPDLTLGAYYSTPKYYLGVSYVHLNAPLIKLNDEKSEFNVRGVMYATGG
                     YDYALVNPKFLIRSSSLVKSDFVTWQAEVSSRLEFDKKYWGGLSYRFQDAVVVFGGLS
                     IMSGLTLGYAYELPTGKMLTVSSGSHELFLSYSFLFDSNRHKNRYKSIRIL"
     gene            complement(23315..23536)
                     /locus_tag="PLN75_01040"
     CDS             complement(23315..23536)
                     /locus_tag="PLN75_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRG02732.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYETIEDIWPDYPSKEDFFFDEEEY"
     gene            complement(23607..24158)
                     /locus_tag="PLN75_01045"
     CDS             complement(23607..24158)
                     /locus_tag="PLN75_01045"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRG02733.1"
                     /translation="MKIYTKTGDGGTTGLIGGSRVNKNDVRLEAYGTVDELNAQIGLL
                     ITHGLKDEHEVFLKSIQNLLFTVGSNLATDTSKTQHKRASLMKEDYITAVEREIDRLD
                     TQLHPLTYFTLPGGTPPAAQSHVCRTVARRAERRIIEMNDIYSVDSKVIIYVNRLSDY
                     FFVLSRVLLMERNVKEIAWKQPE"
     gene            complement(24178..24957)
                     /locus_tag="PLN75_01050"
     CDS             complement(24178..24957)
                     /locus_tag="PLN75_01050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRG02734.1"
                     /translation="MKRLRFILKYFSHLFDAKNTRGHGIHSPYLYRFTQNAIYEKNPF
                     YVFPKIELLRENLKFDERKLFVKDYGTGTDRETRVLDIAKASLKSPKWAQLLYRVVNF
                     TKAQQVLELGTSLGLTAAYMAAVNSEINCVTMEGSDEIAGIARENFEKLEIENVDIIV
                     GNIDEILEMHLAATDKLDVVFFDANHRSEAVMKYFNQCIEHIHPDSVFIFDDIYWSAD
                     MEKAWNEIRKDKRVTATIDLFEIGIVFFNKNFPKKTYKMKL"
     gene            complement(25244..26893)
                     /locus_tag="PLN75_01055"
     CDS             complement(25244..26893)
                     /locus_tag="PLN75_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG02735.1"
                     /translation="MHLSDRTLSDWLEYWAAESPDKEYIVYSDRNLRFSWKTFNERVD
                     NMAKGLIAIGVTRGTNVGIWAQNVPDWLTFLYATAKIGAVAVTVNTSYKSEEVSYLLK
                     NSDMHTLCMTDGAAGNNYTDIIYNLVPELKTTQRGKFRSERFPELKNVVYIGQEKYRG
                     MYNTAEILLLGNNVDDDEFKRLRALTCCEDVVNMQYTSGTTGFPKGVMLTHYNIVNNG
                     YLTGVHMKFTPDDKCCICVPLFHCFGVVLATMCCLTHGSTQVMVEKFDPLITLASIHK
                     ERCTVLHGVPTMFIAELNHPMFSLFDMSSLRTGIMAGALCPIELMRQVNEKMFMDVTS
                     VYGMTETSPGMTQTRVDDTFEVRCTTVGREYEFTDVKVLNPENGEECPPGVQGEICCR
                     GYNVMKGYYKNPQATAQVIDSNGYMHSGDLGVKDENGNYRITGRIKDMIIRGGENISP
                     KEVEDYLYKIPQIKDVQVVAIASPRYGEDVGAFIILKEGAELTAEDVRDFCKDHIARY
                     KIPRYVFFIEHFPMTGSGKIQKFRLREMALELCEKQGIEII"
     gene            complement(27066..27632)
                     /locus_tag="PLN75_01060"
     CDS             complement(27066..27632)
                     /locus_tag="PLN75_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRG02736.1"
                     /translation="MNEVTNKIKTFCTDTGISEEELAERTGLSPEQIKMIIAGDNIPS
                     LSSLIKIARALGVRLGTFLDDSENFGPVVNRSADHQQAATFSSQLSTANSHMDFFSLA
                     SRKAGRHMEPFLIDLKPSAGGEKVSSSHEGEEFLFVLSGSVLVKYGRDNFILYQGDSI
                     YYDSIVDHLVTTATDEPAQILGIVYSPF"
     gene            27882..>28446
                     /locus_tag="PLN75_01065"
     CDS             27882..>28446
                     /locus_tag="PLN75_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRG02737.1"
                     /translation="MKQRFTFFLLFAGILFTSAQKPSQQWLDQKFSMFIHFGLYSVYG
                     GVYDGKPVTRGYSEQIQSFAGIFSDWYGNTARRFNPVKWNPDSIVDLAKKAGMRSIVF
                     TSKHHDGFCMYHSKHTGFNIVDATPYKRDLMKELAEACKRGGIDFAVYFSLIDWNFPQ
                     AYPISSHNADPLTPEHYQFNLKQVEEIM"
BASE COUNT         7854 a         6127 c         5918 g         8447 t
ORIGIN      
        1 aaacaggctt atgaaagccc caaagatacc gttaaatttg attgtcattc ttatctcaat
       61 cataaagatg cggtatggac atggtcattc tcaccggcac caaaatatgt aagtaacatt
      121 catgcacgaa atcccagagt tgtattcaaa gaacccggaa tgtattcagc cactttgagc
      181 atcactcaaa acggacaaac agtttcaaaa actgtgacag atatggtaga agtgagagtt
      241 gctccttcga tagataattg cgacagacct ggcgaactcc ccaaaaacat catgaaagca
      301 acggttgaca gttatcaaag cggtgaagac ggcagcaaag ccattgacgg aaatacatca
      361 acgttgtggc acacgccctg gagcggttcg cctggccacc cacactacat tcagcttgat
      421 cttggagaca attataacat aagcaaactt attcataccc caagaacaga tggttcaaac
      481 ggaaggataa aagattacga attgtacatt tccgacaatg tatccaactg gggaacttac
      541 gtattgaaag gaaccttcga aaacaccagc tcacctacta ctgtagagtt tccggtaaaa
      601 acaggacgct acgtaaaact ggttgcactg agtgaagtga atgataataa ctggacatca
      661 attgcagaaa tcggatttat cggttgccgg gtgacaactt cggtgaacga ttatttcagc
      721 gatgccacac tgcatgcatt ccctatcccg gcaacaaatc tcattacggt aaatctgcca
      781 ttccaaaacg gactgaatgc ctattcatac aatgtattca acacctccgg gcaattatta
      841 aaatcaggaa atgcgggtga aaatcagact tccataagca tagatgttaa tagttatcct
      901 tcgggtcaat acgttgtgat tctcgaagat aaaacaggaa tccattatcg tgtaaaattt
      961 attaaaaggt aatagatagg tttaattttt catggtatgt aaaaaaagct gtttgtgaaa
     1021 acagcttttt tgtttttaaa taacaccgga atcataaaaa gacagagttt tatcaccaca
     1081 tattgatatt taataaaaaa taatgtaagt ttgtgattca aatttatata aatatgatca
     1141 aaataatttc cccttctcta cttgccgccg attttggaaa tttacaggct gactgtgaaa
     1201 tgattaaccg cagcgaagcc gaatggtttc acatcgatgt gatggatggc gttttcgttc
     1261 ctaacatttc gttcggtttt ccggtgatag aagctgtgaa aaagcattgc agaaagccac
     1321 ttgacgtcca cctgatgatt gttgaacctg aaaaatacgt ggagcgattt gtcaaagccg
     1381 gagctgagat tctgactgtt catcttgaaa cactcaaaaa tccgtttgat acgctgcgaa
     1441 gtattcgtga gctgggagtg aaagtaggaa ttacaatcaa tcccgatgtt gaagtcagaa
     1501 acctttatgg ccttgtcagt caggttgata tggtgcttct gatgtcggtt tttgcgggct
     1561 atggcggtca gaaatttatc gaagagactt acccgcgcat cgatgaactg aaagatatta
     1621 ttgcagatga aaatcccgac tgcctgatac agatagatgg aggcgttaat ctggaaaatg
     1681 ctccaaagct gtttgatgcc ggtgtgaatg tactcgtggc aggcagctct gtcttcaaag
     1741 ctcccgatcc ggtgagctat atccatcaat tgctccatca gtaaccccct ctcaaccctg
     1801 atgaacttcc tgcagcgcac tccattcttt cgttttttgc tttttctggc agcaggaatt
     1861 gtgctttttc aatttgtccg gctaccggct gttgcaatag caggcattgc gtcagtggca
     1921 ggcataggtt tgataaccga tttcttcctg ataaaaaaag atattcacta ccgattcagg
     1981 tggattttcg gttttgcaac atttttgata ttattggcgg caggctatac cagttctctg
     2041 atttttgaaa aacaacatgt ttttaacgaa ctgaacgaca gaaaaatata tgaagtagaa
     2101 ctccgctcgg cacctgtaga aaaagaacgt tcattcatga gtaaggtccg gttaatccga
     2161 aagtacgaat cgggcaagga aaaagtttgc agcggaaatg ccattattta cctgcaaaag
     2221 gacagtgcgg caagcgaact tatcataggc gatcgtctgc ttattgaagc agaattccag
     2281 agacctgacg gcgtgcaaaa tcccggtgga tttgattacg ccaactacct gaaaagacag
     2341 gggataaatg cgacagcgta cattccggca gaaaagtgga gaaaaaacgg agaaaacatc
     2401 catttcaatc ttttacggct tgccgatcgt tccaggaacc tgctgttgaa tatttaccgg
     2461 aaattcggca ttaatggcga cgaatttgct gtgcttgcgg cacttacgtt aggatatacc
     2521 gatgctttac aaccggattt actgaaaagt tacagcgcca cgggtgccac ccacatcctt
     2581 tcggtgagtg gtttgcatgt gggtatagtg tatgcagtca tcttttttct gcttggtttt
     2641 ttatcaaaaa caagaagcgg tcgaattatc agagcagtta tcgtttcgct ttttatctgg
     2701 atgtatgcat tccttaccgg actttcgccg gcagtaatga gagctgcact gatgattaca
     2761 tttgtagcag caggtacgtg ttccgacaag aaatcccaga ctgtaaatac cgtgctgatt
     2821 tcagctttcc tgttgctgct gttaaacccg aatctgcttt tcaacatcgg ttttcagctc
     2881 agctattcgg ctgtattgag catcgtattc tttcaaccaa agatttcaaa actacttttt
     2941 gtaaaaaaca gttttttgaa aaaagtctgg gatctgactg ccgtttcaat tgccgctcag
     3001 attggcacag cgccttttgc attgtattat tttcagcagt ttccaaacta ttttctcctc
     3061 acaaattacg tggcaatacc catatccacg gtgattattt acctggctat ggctttgtta
     3121 ttcctgtcaa aagttccgct actttcatcg gcaatcgctt atgctctcaa atggacaacc
     3181 tggtttatga attttctgat cgaaaaaata caggatatac ccggttcaat ctgcataata
     3241 ccgttaaacg tgatacagtt aattctctgc ttcgtggtta ttttttcatt ttcagcgtat
     3301 tttatcaaca gaaaattcgc tccactgatg gcaggtttag catctattct gcttatcctc
     3361 ggtatctaca actaccagaa atacagcagt ttgacgagtt cgaaactgat agtattctca
     3421 gacaaccgcg tgccggtaat caattttacc gacggcaccg acaattatgt ttatacgacc
     3481 gattcagttc aggctgtgaa aactgcttcg tcatactgga aaagcaattt actcagcaaa
     3541 ccaacttatc tgaatgataa tgaatggttt aaagatggat ttgtgagctt cgggaatatg
     3601 cggattttga ttctgaaaga tgattttcta aaatttaaag ttgcaccgaa acctcttgat
     3661 gtggattatc tgattatatc caacaagctc aagcccaaaa tggaacaaat cttaaactgt
     3721 atccatcccg aacatgtcat tgtggacaaa agcatttcag catggtatgc caatcatatc
     3781 cgtgaagtgt gtctggcaag gcaaatcaac tattattcag tagccgaaaa aggagctttc
     3841 atttcacacc tggaacgtta aatttgactc cggcttcaaa atcacaatca aagtcaccgt
     3901 ttttttgtta cttttgcaga ttaaatcctt aaaaacgtgg aaaatgattt ctaaagttat
     3961 agctgaagag ctgattcaca agttaaaaag agaaattgag aatgccgaaa atatcgtgct
     4021 ggttacacac gttggccctg acggcgatgc gctgggttct gcactgggta tgtggcattt
     4081 cctgatgact attgataaaa caccaacagt gattgttcct tctcccccac ccaatttctt
     4141 gatgtggatg cccggagccg atagaatgat tatttattct gaaaaaaaag aagaagctga
     4201 caaagccatt gacgaagcgg aattgattct ggccgttgat ttcaacacgc caaaccgact
     4261 ggctctgatg gccgaaaaag ttgtgaatgc taaagcacac aagattttga ttgaccatca
     4321 tctgaatcca agtcctgatt ttgccaacgt tgtcatttca tatccgcaga tttcatctac
     4381 cagcgagctc attttcaggg caatatgccg catgggtcat ttctctgaca ttaatctggg
     4441 atgtgcagaa tgtatttatg tgggtatgat gaccgatacg ggcggattta cctacaactc
     4501 gaaccacgaa gaaatctact ccattatata cgaacttatt aaactcggag tggacaaaga
     4561 cgacatttac cgcaaagtgt acaacacttt ttcggccgac cggatgcgtc tgatgggcta
     4621 ttgtctgtac aaaaaaatga aaatatatcc cgaatatcag gccgccctga ttacactaac
     4681 tcaaaaagaa ctgcacgagt tcaactacga taacggtgat gcagaaggtt ttgtaaacat
     4741 tcccttatcc attgagggta ttaattttac agtattcatg cgcgaagatc ccgataaaat
     4801 caaagtctca ctccgttcac agggcacttt tccgaccaat aaatttgctt ccgaagtttt
     4861 cggcggaggc ggacacctga atgcatccgg tggagaaagt tttacaacat tacaggccgc
     4921 tgttcgcaag tttgaagaag cactgccatt gtatagagaa tttttagata cccctaaccc
     4981 ctgaaggggg aatctcaatt caaattcaag atttaatgaa aaacaacata aataattctt
     5041 ttgaaaaaga atacccggat aattcatctt taaacttccc cttcagggag gtgaggcgta
     5101 ataaatacat tatcatactg ggtgatggta tggctgatga acccattgca tccattggca
     5161 ataaaacacc gcttcaggcg gcaaacactc caaacataga caaactggca aaaatgggtc
     5221 gctgcggaat gctcgacacc attccccgag gatttgctcc cgggagtgaa attgctaatc
     5281 tgtcggtgtt aggatatgat ctgaatgaat gttttgaagg gcggggaaca ctggaagctg
     5341 caagtatggg cgtagaaatt tacgatgatg aaatggccat gcgctgtaat ctgatttgtg
     5401 tggaagatgg aaaaataaaa agccactctg caggccacat cagcaatgaa gaggcccggg
     5461 aattgattct ttttctccgg aaagaacttg gcaatgaacg ggtgagcttt catcccggag
     5521 tttcctacag gcatttacta aaactgaaaa acggtaataa aaaccttcac tgcacgccac
     5581 cacacgatgt gccgggaaga gatttcagtc cattattgat aaaacctgaa gttcccgaag
     5641 cacaggaaac agcaggtttt ctcaataaac tgatattaaa atcacaggaa ctgctcgaaa
     5701 atcatcctgt aaatcttaaa cgtgcagccg aaggcaagga aaaagccaac agcatctggc
     5761 cctggtcacc gggctacaaa cctacaatga aaaaaatgac cgatattttc ccgataaata
     5821 gcggttcggt catttcggca gtagatttaa ttaaaggaat tggagtatat gccggattaa
     5881 aaaacatcga tgtggcgggc gctaccggac tttacaatac caactacgaa ggaaaagccc
     5941 gggcagctat tgacgcgctg aaaaccgatg attttgtgta tctgcacatt gaagcgagcg
     6001 atgaggccgg acatgaaggt gacgtgaatc tgaaaatcaa aaccatcgaa tatctggaca
     6061 acaggattgt gaaaccaatt atggacgaac tgctgacgtg ggaggaaccc gtgacaatag
     6121 cactgctacc cgatcatcca actccctgcg taatgcgtac acacaccaat acacctgtgc
     6181 ctttcatcat ttacagaacg ggaaatactc cggatgaaac agaaatttac gatgaatttg
     6241 catgtaagaa tggcagttat ggtttactga aaggaaatga atttatcagg gaaatattca
     6301 gacaataatt ttccacaaaa ggaactctgc aaattacagc aaaaaatgat tcttcgttat
     6361 ttttaaaaga gatttacagt ccatttaatg accattattc caaatataga agtaacagat
     6421 tgccccaaaa gacattcgga ggtctttcgc actgaaggaa taaaatacac cggctcaaaa
     6481 ttaaaaatcc tgcctgacat catgcagctg atacatcaat tacctgacgt caggacggta
     6541 attgacggct tcagcggttc aaccagggtt tcacaggcac ttgcccgcac gggatatgat
     6601 accacttcaa acgatatatc cgtgtggtct gaagtactgg caacctgcta cctaaaatca
     6661 acaaaaccag acagttatta ccgcgaaata actgattatc ttaatgccct gccaggttcc
     6721 gacggatggt ttacagaaca ctacggaact gctgaagaag gacaaaaaaa accatttcag
     6781 ctaaaaaata cccgaaaatt agattccata cgtgatgaaa ttgaccggct ggaattagat
     6841 tgggaagaca aatgtgtgat tctgacaagt ctgatatatg ccctggataa gattgacaac
     6901 accctgggac attatgctgc ctatctgaac aactggtcca aacgttcatt caatgatttg
     6961 accctgaagt tacctcagcg attccggtgc acgggtgaaa actctgtcgt acgaagtgat
     7021 atttttgata cgatagaaaa aaacaaattt gatttggctt attttgatcc gccatacggc
     7081 tcagccaaca ataaaatgcc tcccagtcgc gtaaggtatg catcgtacta tcacatctgg
     7141 actacggtga ttctgaatga caaaccaacg cttttcggca aagcaaaccg acgcacagac
     7201 acacgtgatg gagtttcagg atcggttttt gaagaattca gaacaaacga aaacggcaga
     7261 tacctggcag tggaagcact cgaaaaacta atcagagaaa ccaaagcaca ctacatactg
     7321 ctttcataca gttcgggcgg aagagccacc aaatcagatc tgacagatat catcctgtca
     7381 tcgggcagac tgctgaaagt aatcgaaatt gactacacca aaaatgtcat gtcggaaatg
     7441 agatggacca acgaatgggt aaacagcaat ggtaaacaca aggaatatct tttactgaca
     7501 gaaaaagtat aacttctctt tattaataaa aatacaggac catcaataat taaaatggct
     7561 atcagcatga aatattacag tacaaacaaa aaaacaccga acgttacact cgaagtggcc
     7621 gtaattaaag gactggctga ggacaaagga ctttttatgc ccgaaagaat tgaaaaactt
     7681 ccggatgaat tctttgaaac cattggaaca ctttcatttc aggaaattgc ctttgcggta
     7741 gcccaagcct tcttcggaga agatgtggag actgcagcac tgaaaaaaat cgtgtacgaa
     7801 acgcttccgt ttgatacacc gctggtggaa gtagaaccca atatcttcag tctggagctt
     7861 tttcacgggc ctacactggc tttcaaagac gtgggagccc gattcatgtc gcgcatgctg
     7921 gcatattata tcagccggca gtcaaatgca gatgctgtaa atgtgctggt agccacttcg
     7981 ggcgataccg gaagcgctgt tgccaacgga tttctgggtg tggaaggcat tcgtgtgttt
     8041 gtactttacc cgaaaggcaa agtcagtaaa attcaggaaa gtcagtttac caccctcgga
     8101 caaaacatta cagcgcttga agtggacgga actttcgacg actgccagcg actggtgaaa
     8161 tctgcctttc tggatgaaga actcaaccga aaaatgaaac ttacttcggc caactctatc
     8221 aacgttgcac gcttcctgcc acaggctttc tactatttct acgcattctc tcagctacgc
     8281 tccatgcatc cgaagcaaac ggcagaagag ctgggaacag ttttttgtgt tccgagcggt
     8341 aatttcggga acctcacagc cggtctggta gccaaacgga tgggattgcc cgtaaagcgg
     8401 tttattgcag ccaacaatcg caatgatata tttctgcaat acctgaaaac aggaatttat
     8461 tctccgaagc cttctgtggc gaccattgcc aatgccatgg atgtgggtga tcccagtaat
     8521 tttgtgcgga tactggattt atacggacac tctcacgaag ccatttccaa agaaataagc
     8581 ggcgtgagtt acacggatga gcagattact gaaacaatga gaaattgttt tgttgaaacc
     8641 ggatatctgc tcgacccaca cggtgcctgc ggttatcagg cacttaaaga cgaactcggg
     8701 gaaaatgaaa acggcatttt tctggaaaca gctcatcctg ctaaattttc cgaaacagtg
     8761 atgagtattc agggattaaa tgtggaaata cctgcaagac tgcttgaatt tatgaaaaac
     8821 aaaaaacaca gcatccctat cggaaccgat tttgaagaat tcaaaacatt tctgttgaaa
     8881 taaaaaaaac acacgggcgg catcctgaaa agagccgctc gtgttttttt tttcgccact
     8941 gaatttttaa aatttataca taaaaccgat gccgagtatc tccttaaact gcactcttgg
     9001 gctgtcatcg tcaattgacc cgctctgatt ataatcaatt cttacatcgt ggtcgtatat
     9061 cagttgcgtg ttgaggctta cggtgatgta tttgttaacc ttcattccaa gcagcgtttc
     9121 ccagttcaca tctacatact gagggtcttt caggtagttg gaaaacaaat caacctttgt
     9181 gccgatggac atattctgga aaaaaccttg tttaaaatca ctttttgcga aagcgagcct
     9241 tacatagccg cctaattctt ttttcatttt ttcacctttt gccactccaa atgccccttt
     9301 ttgtgataat gcctcatcca gcacaaatgt aagcctgcct gtaagcggcg aaaggaatgc
     9361 attgaaatac gtattgggta tgtaattcaa ccccaatgct gccagcaaat atccgggagc
     9421 catgaaattc gaaattttat ccacatcgtt cggatagtta tatccgggtg aaaactgagt
     9481 gcggaaattg agtaaacccg catagtaaaa atttttgaaa gctttccgcc cgtatttaga
     9541 gttaaattcg atgcggtcat ccgttttcat gattccatcg tacgacgact ggcgtaaaaa
     9601 tccatagcca aggtcaagcg tgttttccca aacgttcagt gtatctttat acaccgcaaa
     9661 aaagttaaag agtccgttga gtgccagcga gttctgacca cctgcagccc agtttgtaag
     9721 cgaagtctga gaaaaattca gccccgaaaa gccgcccttt ttccaggatg caaccgtatc
     9781 ggtttccact tttttcagtt tagactctgc atcagttatt tgcgaagagg caaccatgcc
     9841 gaatataagg tacagaccca gaaataatgc tatttttttc atattcagat taatttttga
     9901 tgattcattg ctgcaaatat agttaatttt taaaataaaa agcaaaaaag caaaaaaaac
     9961 ctgctcgaaa ttttccggca ggtttttttt ttgcttttag cgttaatccg ttatgattca
    10021 acgggttcaa gttctatcat gataaaatcc ttcggtaccc tctcgcccgc ctttacgttg
    10081 atttttttta tcctcgccgt aaacggcata tccacccggt tctgcatttt catagcttca
    10141 aggataagca atctggtgcc ttcttccacg atttttccct cttttacatc gatggaaatt
    10201 attgtaccgg gaatatacga ctggatttcg tacggacttg gatttaccca gaatttccgc
    10261 tgcatgtact tcttggtgag ctgagtcttg tatttacggg ccgtaatggc aaaatcaaca
    10321 tattgtggct ctggcattgc cttgctttct gttttactgt tcccgggagc tgaattcttc
    10381 atgtttttca gttttcttgc ttcctcagct ttttttcgct cagcggctct tctttccatg
    10441 cgcgcatcac gctcttcttt agcttttctg gcttcatgcg cttttctggc agcgtgtatt
    10501 ctacgggcac gttcaaggat attcttgctc ttttccttct tctcaagcag agcctgcttt
    10561 tcacgggcgt ttttctctga taaagtcaaa tttttaacct gagcttcatc ggctttggtt
    10621 ttatttctat cattttgcgg catggtcaaa ttcttttttt attataattt tcataaaagc
    10681 aaaaaaacgg aacgaaatgt tagaacggtg gaatcccgtg tttcttagtc gggcgcgaat
    10741 cgtctttatc tcttgaaacc gataaagagt gaagcagcat ttcacgggtt tcatctggtt
    10801 cgattacggc atcgatatat cctttggaag ctgctacaaa tggatttgcg aatttttcga
    10861 catattcttt cactttttgc tgtcgcatag cgtcttcatc ttcagcctcc atgatttctt
    10921 ttcggaaaat gatattggca gcaccttcgg gtcccataac cgctatttcg gcacttggcc
    10981 aggcatacac aaaatcagcg ccaagatgtc gggaattcat ggcaatatac cctccaccgt
    11041 atgctttacg caggattacg gttaccttag gcacagtagc ttctgaataa gcatacaaca
    11101 ttttggcacc gtggcgtatt actccggcgt gctcctgatc cactcccggg aggtaacccg
    11161 gcatgtcttc cagggtgata atcgggatat tgaatgcatc acagaaacga ataaagcgtg
    11221 ctgccttgtc agaagcatcg caatcgagta ctccggccag atacatgggc tgattagcta
    11281 cgaaacctat ggtttcgccg tccatccggc caaaaccgat tacaatattg gatgcccaga
    11341 gttcctgaac ttcgaagaat ttggattcgt caacgagagc caaaatcact tctcttacat
    11401 cgtagggttg tttcggatcg gcgggaatga tattttcaat tttttttctg aatttcggat
    11461 ctttcggttc aatgctttgc gcgcgttttt ggttgttgtg tggaatgagt gaaataagcg
    11521 ttttgatttg tttgaaacac tccatttcgt tcgcagcgta gaaatgggca ttaccggtaa
    11581 tttcggcatg tacacgcgct ccaccgaggt cttcctgtga aattttttca cccagcaccg
    11641 tttcgataac gttgggtccg gtgataaaca ttttcgaaat attctccacc acgaatacaa
    11701 aatcggtcag tgcgggtgag taaacggctc cgccggcgca gggaccaagt ataactgaga
    11761 gctgaggtat aacccccgaa gccatggtat tgcggtagaa aatctcgccg taacctgcca
    11821 atgctccgat tccttcctga atacgggcac cgccggagtc gttgattccg atgcacggta
    11881 ttttcatttt cagggcatga tccattattt tggtaatctt acgagcatgc ttcagcccga
    11941 gcgatccacc catcacagtg aaatcctgag cgtagataca tacgggtgct cctccaatgg
    12001 ttccggttcc ggtaattaca ccgtcgcccg ggagttcttt ggtttccata ccgaagtttt
    12061 tctcttcatg ttctacaaac atatcatatt cgtgaaaaga accttcgtca agtaaagcaa
    12121 gtattcgaac acgtgcaggc attttaccca ttgctatctg tttttcgata gctttgtcgc
    12181 cgccgccttt ttcaacgatt gctttcttct ggcgcaatgc gatgatgttt ttctttgaag
    12241 acatattgtt ttattttgtt tgtttatatt acctcaatat ttcagactgc aaagataata
    12301 cttatttttc aaataaataa tgacgaagcg atacatcagg aaggtgtccg cttctatctg
    12361 caccggctaa tgatttcaga atgtgtcttt tagctgcatt ataacctgct gaatacatgg
    12421 cattatcagc attctcgagc gtaactttca attcgggtac gaaatccttt tctatcatac
    12481 atatgttgtt cagatacttc attgaaagta cctgtacaga tacactttgg cgagttgaaa
    12541 gcatttggtc aaaacacaaa ttgattaact ccactgaaac gggtaaacaa acaggaaggt
    12601 taaaaagtat cgaaagacag agacgctgga gcccctgatg cttgtttcta agcacaaaac
    12661 cgactaattg tctgttatta tcttcagaaa tgagatttgc atcgtgctca ctcacttttt
    12721 caatcaccca ggcggcatgc caggctattt tttcatcggt atcgaaaatc aaactgaaca
    12781 catactccaa ctgctcagca tcatgcagca agagggagcg tatcatttct acctcctttt
    12841 tgcccatatt accggacagc accgtccgaa tgaaattgct gtcttccgac atgagatttc
    12901 tgaattattg aaatactatc tctttcacaa cgtcggcacc aacgtatttg ttcagtgagt
    12961 tttttatctc atcgcgggtc agaaagagtt cgtgacgcag aacggaagat gaaagtgtga
    13021 caaaaagttt ttttttgttg aaataaatag ctgttgtata gcgcattatg ttatctccga
    13081 gcacggcaga ccatgattgg atcactctgg tttcgtagag cttttcatcc aggtgatttt
    13141 gcttaagcac ctgggcgaga atttcgttta atttttcagt attttttctg cgcatagttc
    13201 accacttgag ctaccggtgg ttctgttttt ggttttacct aagtttcagc actttattta
    13261 caacagcacc ctgatcatag ggcatctgat tcagatcata cagggattgt aacttaattc
    13321 cgtatttttg tgctatccga tacatgtttt cgcccggttt aatctgatgg gtttggtatt
    13381 cggctgatgc cttttttctt ttggctgaca aatatactac ggttccgggt gtaagttcgt
    13441 gcgattgatt cagatcattg taattcagga ttttattttc tttcagatta aattcgtggg
    13501 ctattcctgc ataagtatca ccggcttcgg cgaaaacaca ttttactccg ttattccgga
    13561 aaagattgtg tcctttgagc gttgtaacag aagcttttcc ccaggtgtat gtttcgcggt
    13621 ttgattcaga atccgctttt ttcatatcgg gtttgctgcc tgtatcgtat ttatgcaggt
    13681 cataatcttc aataagttta attaatttat acgcatatcg ctcatcggta gcatatccgg
    13741 cagctttcag tccgtgtgcc caacctttgt aatcagtagc gtcgagtccg aacagagatg
    13801 catagcgtgg gcgggtgatg agaaattgag aatgatcctc aaaagattct ctggctgagt
    13861 tgtattttct gaaacattcg ttgcgtttat cgtcgtccgc atacactttt tggccggtcc
    13921 agtcggaaca tttaatcccg aaatgattgt tggagatgct caccagtttg ccttttcctg
    13981 ctgccgattc gagtaatccc tgagcaagag tgatactggc gggtatttta tgtagatttt
    14041 gctgttcaac tgctatttca gaatatttgc cgatatactc caggtacgcc tgatgcattg
    14101 tttgagcatt ggccgggagc aaaccgagca tacaaatcac tgctaatacg atttcttttc
    14161 tcattgctaa tcatttttcg ggtataatga ttgcaaaaat aaaactaatt tgatgaaaat
    14221 gtggttttgg ttaaataatt ttatatattt gtactttaag aaaaaaagac ttcgcgttat
    14281 gcaatattta aaatacgaaa cgagaattac cgagtaccta ccgaatgaaa tccctgccag
    14341 atatcaatca ttaattgctg aggctaaaaa tcagacagaa aaagcatacg ctatttactc
    14401 gcagttcagg gtaggcgctg cgctgcttct ggaaaacggt atgacagtaa ccggaaataa
    14461 ccaggaaaat tcagcttatc cgtcaggatt gtgtgcagaa agaaccgcca ttttctacgc
    14521 caactcacaa tatccggatt ctgccgtgaa aatgatagca atagcagcat ttgccggagg
    14581 tgaatttttg aaatcaccca ttactccctg tggcgcctgc cgccaggttt tactcgaaac
    14641 agaaacacga tttggtaagg atatagaaat catactctac ggcacagaaa aaacctatgt
    14701 acttgaaaat gtgaaacaaa tgctccccct gagtttcaac ggggacagtt tgaaataata
    14761 aaaaaagctg tatgtaacct ccatgaattg gaggttccat ccgattaaaa aaaaagttat
    14821 acggatgaaa aaagaaatgt ttacaataga atatccactg gaaaattctt cggaaaattt
    14881 actctggaca atgattggag atccgctcgg attggcactg tggttctccg aaggtgtaac
    14941 agttgacaaa aatgaatata ccttcagttg ggatgatcac taccagactg ccattcttca
    15001 ggaagtgagg gaaggaaaaa atattcgctt tcagtgggaa gaagataaag ataccgatgc
    15061 ttacttccag ctggaaatta ttacacagga cctgagcgga catattggtt tactggttac
    15121 cgactttgct gccgctaaag acaaagacga cgccatcatg ctgtggaaca agcagataga
    15181 tgacctccgg cgaaaagcag gcatgtaagc agaagaaaaa gtaacctatc agtagaccga
    15241 ctgctgcaag atatcagaag cgaaagaaaa tgaacttcgc gttcgaagaa acagctatgt
    15301 caaatcaatg cggttttgca gacaaataaa ctgctgacct gtacaattta tgctatctcg
    15361 ccacttccta tgcaaatcct cgaagcggca tcgtacacca ctccaaactg acctgaagca
    15421 attccctgta acttttctgc cgaaaaaaca cgataaccgg tatgtgtttt cataattact
    15481 cctttggtaa attctggtgt gtgacggatt ttgaaagtga tatccactcc tttttcaatg
    15541 tcgttccagg gattctcggt tataaaatgg aagtcgttca tgcagaattc tcttccatat
    15601 tgcgtttcta catcgtatcc gcgtgaagcg taaacaatgt tgcccggaac atcttttttg
    15661 attacatacc acggtccgcc cgaaagtcct aatcctttcc gctgacctac ggtatgaaac
    15721 cagtatccac gatgggttcc aagtattttt ccggtttcaa attccacaac cgggcccggt
    15781 ttttcgccca gataccggcg tataaaatca ttgtaattta cctttccgag aaaacagatt
    15841 ccctgactgt cctgacgttt ggctgaaggc aatcctgctt tttcggctat tttccgaact
    15901 tcatttttca tcaggtgtcc gaccgggaac atcagttttg acacctgcag cgagtcaatc
    15961 tgtgccagaa aatcggtctg atcctttacc ggatccttgg ctgttccaag aaaagttttg
    16021 ccgttttctt cgattgtggt ggcataatgt ccggtggcag tcttgtcaaa atcgtttccg
    16081 tagcgctctt cgaagactcc gaatttgatt agtttattac acatgacatc cggattggga
    16141 gtcagtcctt ttttaacctt atcgatggta taagctacta cggtatccca gtaatctttg
    16201 tgtaaatcga ccacatggag cttacaacca tattttcggg ctatcagagt agccatttcg
    16261 atgtcttctt cggatgagca atgcagcaat tcgtcatcat cctgccctat tttgatgtaa
    16321 aataacgccg gagtaattcc ctgctctttg agcagatgaa ccactaccga actatctact
    16381 ccaccggata ttagcgctgc tatgttcatt tcaggttgat gtttgaatgg ttaatttgtg
    16441 aaattcgtta aaaattcgtg taattcgttc tttcaaatat aataatccga caaatcaatc
    16501 aaacctgctt ttacagcata tttggtggct tcgtacacgt tgttgacttc aattttccga
    16561 aatatatttt tccggtggct gatgatggta tgcatgctcg aatgtcggat ttcagcaatt
    16621 tcctttgttg tttttccgga ggcaatttct ttaagaattt cgagttcggt tgcagtaaga
    16681 tgtgttctgc ctgcttcggt tttttgtttg tatacgttgc tcagcaacac attggaagcc
    16741 acactgcaaa catatctgct ttttcggatc acttgcagga atgctgtccg gatttcgtcg
    16801 gccgatgaat ccttatataa aatgctgaaa tcaggcgaat taaaataggc tcgccggata
    16861 aattcatcgc tcagttcgga tgaaaaaagc agccagtgta cgttgggata cgcaataccg
    16921 atattaaaaa gctcgtcgac acttctgaaa tcaaatagcg aatagtcaag caccactacg
    16981 atgaaaacat ctttacttaa ttcagctaac agttcctttc tggaaagtac gtctttaaat
    17041 tcaaaatgtc ccatccggga gagcacattt cgcgctccct ctctggtaat atactgattg
    17101 tcagccagaa taattctact cacctccatc gttttgtact ttgtattcag tttgcaaagt
    17161 tagttatttt tgagcaacaa aaaaagacgg catcgcggcc gtcttttcca ttgattataa
    17221 ccccaaatca attaattttc ccacaaatcc tgttcgaagt tcatgagttc ggtttcaatg
    17281 cgtttctgtt cagccctcag ggagttttca tcgagtgaga actccaggag catgcggctg
    17341 tacatattgc tatcacccaa cagatagctt gaatatagat tttgagagaa aaattcatcg
    17401 taagtcattc gctgactgtc gtttccattc ggaattacga gttgacgaat gagatatgga
    17461 cgcagttcgt cgaaagaaaa ccagcaaagc accgagtttc tgaaatacgc cattgagtcg
    17521 ttgtttgcca tattgtcggg gtgcagcgcg tacatcggtg caatggcaat tattttggaa
    17581 tacaaacgtg aagtatgcgt atcaaagaaa atgacttcct gaatgaggaa tttaagctga
    17641 ttgcgcacat accgcatgta ctgatctgag tttatggtgc gttgttcggt aatgggatcg
    17701 acctgaatca ggttgttttc cacactttcg tcgtatgcaa atacgcgtga gagttcttcg
    17761 ccggtgagta tgtcgtcgaa ttgcggtttg atatcgcgcg gattgcggct gtacacattt
    17821 attccgctca ccacagcgtc aagcatcagc cggaaaagac tgcggtgctg ttcgttggga
    17881 cgagtcggga aataaagctg aaagttttgc ttgtcgcgca tgtcaatcac gcggtacacc
    17941 acgcgcgacc acgttacatc ttcggccctg tggtttatca cagcgatggt atcagccagt
    18001 gcatcgagtt ccatggtctg gagacggata tttccggtct gatcaaaaaa tgaggcgttg
    18061 ggatcttgtg cctgcatgtt acccgaaagt gtgagtaacg tgaaaacagt gaatatcaaa
    18121 ctatattttt tcatacatta atttattttc tgaatgtcaa taacttatct tttgttgtgc
    18181 tgtatgtctt ataattctac ctgtataact cccagaccgc gtgtttttcc atccggtcct
    18241 acggttttga tatttttaaa tgtgataata tcgccgcctt cgagtccttc cagatcagat
    18301 ttttgtttcg cgctaagctg accgctgctg cttgtagcta ctcctgctat ggttagctga
    18361 gtaaatgagg tgatttcgaa ttttgcctgt accagcgcat cttcgccata actggctatg
    18421 atacgtgcat tttgcagttg ttttcgggtg acacgtcctt ccttaatttc gcggggaacg
    18481 ccgccggcat cggtatactg aagataggct atcgggtcgg gcaggtattt cacgcggaaa
    18541 actccgcttc ccatctgctg ttcacgtcct tcaaccaatg ctgctacaaa aatggtgatt
    18601 tcaccatcct ggttagctct tactatgtag tttccacctg aaattttagt tgcagatccg
    18661 ccgtccacag ttacccgaac attgtcggca gctattccgg gcaccgaaac gctgaaacgg
    18721 ttgtcgatac cgcggtaaac cacgttcaaa tcttcgttgg aaattgtggc agcaggtttt
    18781 cctacggtgt attcctccgt aaaaggataa gaaataaatc caccctgatt gtcgggcata
    18841 cggatagtgc cggaatattt gaatgtacct gtctgggaag gaacaaattc atatttcccg
    18901 aattttttgg cgcctacggc gggaattctt gtaccgttta caaaatattc atactgccgg
    18961 gttgagtcgg tggctgccag aataatgtca gcggaatatc tgcctccctg aatgatgtat
    19021 ctggagtttg gcactacgaa agcctggact ttattgaccc tgaaatcctc gaaatcagtt
    19081 ctcgatttga ggtattgtac agtttcagct tctgccgagc gaatatcgga ttggtatttg
    19141 gtaagaatgg taatggcagc cgaaacaggc atcgattcga acagtttgct ctcccatctt
    19201 gaaccgcctc ctgtggcgaa cattgcgctg tacatttcgg ctttcgaagg attggttttt
    19261 ttcagaaatt cacgatatgc ttcgattttt tgtctcagtt gttttccgtt acctttcacg
    19321 atggcatatt ctccggctgc gtcaatatct tcacgggcta tgatattaat ggcattttta
    19381 tctgcttttt tcccgtcggc aattttaaga attccgtatt tgaagtcttt caggaaatta
    19441 tagagttcat cggaattttt cttcacctca tttgcctgat cgagccattc ctttactttg
    19501 gccggatttt ttttattcat aaactcgaaa tcatcgtaca tcgaattatt tcgtgtttcg
    19561 gatgaagcga ttgtagtacg caggctacta tccaccaacc cgaatccgtt caggatttcg
    19621 cttgacacgt tgagtgccag catggcagtg agtaccaggt acatcatgcc tatcattcgc
    19681 tgacgcgggg tttcgggaca attctttgct ccactcattg gttggatatt gagttattga
    19741 gttgtttttt actttttatt ttctccgggt cggtatttct acgatagcgc attcaacata
    19801 tttccataaa tggcattgag gtcttcaacc tgccgggtaa gtttgcgggt agccgactgg
    19861 tatcgctgac tttctgcctg agctgcagct gctgcttcct tcattttgtc catatcggat
    19921 gctaccccgt cgatatgcgt cgtagcctgt ctgatggctt ctgcctgctg attcagactt
    19981 tcggtctgcg actgaatatg gcggagctgt atttcataga ttgaattgat ggtggagagg
    20041 cttctgttca cattatccac attcacagaa taatttcttg tattgctcac cacgtgatcc
    20101 atttcggtat tcacaccctg ataggatgca gcaagcgttt cggcggtggt attgagtttt
    20161 tgctgcgtat cggcaaaact tgcagccatt tcggaggctg tttcgatatt cctggcaaag
    20221 ccattggcgg ccaatgccac atttgccagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnt tgctccagtc gtattcccgg tagggtctgt cgaatatgcc cagcgcaaaa
    20401 atgacagact caaccgacat gccaattccc agcataatgt tgccaatcgg ataatgtaat
    20461 atcttaaaca tggctcccaa aattacaatg gatgcgccta aactgtaagc tgcagatatt
    20521 attcttctgc ctgaatagga attccaccac ctataaaatt tactatctgg tttcctcagc
    20581 ttcattgttc ttattatatt aaaaaatctg ttataattta gttttttcaa attatgaaaa
    20641 aatggttttc agccatttta cgcatactgt taatcgcctg tatagaatct gacacaacga
    20701 aaaccgatgt aaggacgact ttcattctgg tattgatatg tttttactcc gttctgcagg
    20761 taatatgcaa tgtctttcca ggaacctcct ttcaccacct tacgtttgag aaccggagga
    20821 tcgtcatttt tagagttgta ctgataattc ggattaactt ccgatagtat tgcgcccgac
    20881 gagggattgt aggcagatga tgtccattct gccacattgc ctgccatgtc ataaagaccg
    20941 taattgtttg gagcaaatga acctactttc atggttgttg ttcctgagtc gtttccaaat
    21001 ccggctcgtg ccggtttgaa attagccagg taacagcctt tggaatcacg aacataattg
    21061 cctccccaag gatacagggc aagttcgcgt cctccacggg cagcatattc ccattcggct
    21121 tcggtgggca gtctgaactc ttcgcctctg accagattag taccgtaata cagctttgta
    21181 cgccagttgc agaatgcctg cgcctgttcc caggtaacac ccactacggg gtattgcgaa
    21241 aaagctccgt gagagaaata cattttcatt ttcggatcgt tgtaggtgta ctgaaaatcg
    21301 cgtacccagg ctgccgtatc agggaaaaca ttcacaatgc ggctcgagaa aaaatcgctg
    21361 cgggtctgaa gtggtttggt aatggttaca ttgcgcatca gcccgttttc atccacatat
    21421 ccgctgtcac gtcgtacctt cgctcccgct acaaattttc ctgtatttat atcgtaccgg
    21481 ttggagggta aagcagcttg ttcgtagtcg taccaggagt aaatgtagcg cagcttgctg
    21541 ggatcaattt cctttttgcc tcccagcaca tctccaccct gatagtataa tgcattcagg
    21601 atatcctgta tttcttcatc ttttgaattc cacggaattt tttccttcca gttcagcagc
    21661 gcttcttcgg gagtcgcttc gtccacatct cctttaatgg gtttgcggaa ttcatccctg
    21721 ccggccatta cgagcatgcg cagtgcgacg gaatctctga cgtaattcac aaattgcttg
    21781 tattcgtcgt ttgtgatttc cgtatcgtcc atccagaatg cattgagtgt tactttgcgg
    21841 ggttgctcgc caacttcacc aaataccgac tgatcattcg gacccataat aaacgatccg
    21901 gctttgacgt acaccattcc gtaaggattg gcatctctga aagagccttt atttccctga
    21961 ccgaccaatt ctcccatggg tttgttgcac gaaaccatca gggaagctat gaataatact
    22021 ggcagaattt ttttcatatt ttagctgcaa acttttttat ttgtttcgca aattcgctgt
    22081 ttgattttct attatataaa acggcataat tgcaatttca ttatttgtat ttaaaattca
    22141 atgttttttc agaaatgttg tcatttttct attacaagca aaataataac tcgattttta
    22201 caaaattctt atgcttttgt atctgttttt atgtctgttt gaatcgaaaa gaaagctgta
    22261 agacagaaaa agctcgtgtg aacccgaact gactgtcagc attttgcccg taggcaattc
    22321 atatgcatac cccaatgtga gtccgctcat aatactgagt ccgccgaaaa caacgactgc
    22381 atcctgaaaa cgatacgata aacctcccca gtactttttg tcgaattcca accgcgaaga
    22441 gacttctgcc tgccaggtaa cgaaatcgct ttttacaagt gatgatgaac gtatcaagaa
    22501 ttttggattg accagtgcgt aatcatatcc tccggtggca tacatcacgc cccgcacgtt
    22561 gaactcagac ttttcatcgt tcaatttgat gagcggagca tttaaatgaa cgtaagaaac
    22621 tccaagatag tatttcgggg ttgaataata agcgccgaga gtgaggtcgg gtgccattcc
    22681 ggtttcatca gcagcaggaa tggctgcatc acccagaaag tcgtgaaatt cggagttgat
    22741 tgttgcattt ttgatgctgt cgtacacgaa tccgacgctc acaaaaccca ggtccagacc
    22801 gaggctggcg attcctttgc ctattttacg cttgtaggca tattgcagat gggcagactg
    22861 attggtgaaa gcaccaattt tgtcgttcag gaatttaatg cccagtgcgt gagttacttt
    22921 ttcaccggat ttaaaaggag cgttgacggt gaaataggtg gtttgagggg cgccgggcat
    22981 tccaatccat tggatgcggt gctgtccgga tacgttgatc atctgattct cacccagtga
    23041 ggcgggatta aacgtgggtg agtgaaacat gtactggctc atttgagcct caaactggga
    23101 gaatacaggc aggcaaaata cgaacgacaa cagcattgct gtaatgcatt tcttacttgt
    23161 attttcaatt tgtataatca tccggtacga attttcgaaa atcttatgaa ctcaaaaaat
    23221 aaactctaaa gatttcaaaa aattgtatcg taaagccaaa ttgtttgctt attcaataaa
    23281 aacaagctcc aaaaatgtga atacagaaag taagttaata ttcttcttcg tcaaaaaaga
    23341 aatcttcttt acttggataa tcaggccaga tatcttcgat tgtttcgtaa atttctcctt
    23401 catcctcaat ttcctgcaaa ttttcaatca cctcaagcgg tgctcccgaa cgtattgcat
    23461 aatctatcag ttcatccttt gtggcaggcc agggcgcatc ttcaatcttc gatgcaagtt
    23521 ctaaagtcca atacattttg ttctaaatat ttaggttgat tctactgttt tttcaaaagg
    23581 agtgcaaaaa tataaaattt attttattat tcaggttgtt tccaggcaat ttcttttaca
    23641 tttctttcca ttaatagtac tctggataaa acaaagaaat aatcggacaa tctgttgacg
    23701 tatataatca ccttactatc aactgaataa atgtcgttca tttcgataat ccggcgttcg
    23761 gcccgtcgcg ctacggtgcg gcacacatga ctttgggcag caggcggtgt tccacccggc
    23821 agggtgaaat atgtaagcgg atggagctgt gtatccaacc ggtcaatttc cctttctact
    23881 gccgtaatat aatcctcttt catcaaggaa gcacgcttgt gttgtgtttt tgaagtatcg
    23941 gtagccaggt ttgaccccac cgtgaaaagc agattctgaa tactcttcaa aaatacctcg
    24001 tgttcatctt tcaatccatg tgtaatcaga agtccgattt gagcgttgag ttcatctaca
    24061 gtgccgtaag cttcgagccg tacatcgttt ttgttcacgc gcgaaccgcc tatcagtccg
    24121 gttgtgcctc catcgcctgt ttttgtatat attttcattc ctgaaattta atgctggtta
    24181 taatttcatt ttataggtct ttttcggaaa atttttgttg aaaaacacaa ttcctatctc
    24241 aaaaagatca atggtagcag tcacacgttt gtcttttcgt atttcgttcc acgctttctc
    24301 catatcggcc gaccagtata tatcatcgaa aatgaaaaca gaatcgggat gaatatgttc
    24361 gatacactga ttgaaatatt tcatcaccgc ttcactgcga tgatttgcat caaagaaaac
    24421 gacatcgagt ttatctgtgg ctgcaaggtg catttcaagt atttcgtcga tgttgcctac
    24481 aatgatatca acattctcta tttcgagttt ctcaaagttt tcgcgtgcaa ttccggctat
    24541 ttcatcactt ccctccattg tcacacagtt aatttcggaa tttactgctg ccatgtaagc
    24601 agccgtcagt ccgagcgatg tgccaagttc aagcacctgc tgtgctttgg tgaaattcac
    24661 cacccggtaa agcagctgag cccatttagg acttttcaat gaggcttttg caatatcaag
    24721 tacgcgggtt tcgcggtcgg taccggttcc gtaatctttt acaaaaagtt ttcgttcgtc
    24781 aaatttcagg ttctcgcgca gcaattctat tttcggaaaa acatagaacg gattcttctc
    24841 gtaaattgca ttttgagtaa agcgatacag ataaggcgaa tgaattccat gtcctctggt
    24901 gtttttggca tcaaagagat gggaaaaata cttcaatata aaacggagtc ttttcatatt
    24961 ttttgcaaaa ataagaatat taaccaatat tcaaaaagaa ttgatcttga cttttcttaa
    25021 aacaatagaa aacatagcat gatttatact tattctctta tttttcggaa acatagaaaa
    25081 aatatcttcg atatttttaa actattgtta tcgaaatcat ttaaaacagc acgaaaatta
    25141 aataaaataa gactattgat ctctatgttt taaaagtcat gacgaattca aaataaaagc
    25201 aggaattacc ctgcatttat cattttaaat tttcttccgg ttttcagatt atttctattc
    25261 cctgcttttc gcacagttcc agtgccattt cgcgcaggcg aaacttttgt attttgccgc
    25321 tgcctgtcat gggaaagtgc tcgataaaga acacgtaacg cggaattttg tatcgggcta
    25381 tgtgatcttt gcagaaatcg cgtacatctt cggctgtaag ttcagctccc tctttcagaa
    25441 tgataaaagc acctacatct tcgccatacc tgggtgaagc tattgccaca acctgcacat
    25501 ccttgatttg cggaatttta tagagataat cttccacttc cttgggcgat atattttcac
    25561 ctccgcgaat tatcatgtct ttaatgcgtc ctgtaatacg ataatttcca ttttcgtcct
    25621 tcaccccgag gtcacccgaa tgcatgtatc cgtttgaatc aattacctgc gcagtagctt
    25681 gaggattttt ataatatcct ttcatcacgt tgtaaccccg gcagcagatt tctccctgaa
    25741 ctcccggggg acattcttcg ccgttttcgg gattgagtac tttcacatcg gtaaattcgt
    25801 attctctgcc cacagtggta cagcgtacct caaaggtatc gtccacgcgg gtttgcgtca
    25861 ttccgggaga agtttcggtc atgccgtaaa cgctggttac gtccataaac attttttcgt
    25921 tcacctgtcg catcagctca atggggcaaa gtgctccggc cattattccg gtacgcagcg
    25981 aactcatgtc aaaaaggctg aacatgggat ggttgagctc cgcaataaac atggtgggca
    26041 caccgtgaag caccgtacac cgttccttgt gaatggatgc aagggtaata agcggatcaa
    26101 atttttcgac catcacctgc gtgctgccgt gagtgaggca gcacatggtg gccagcacca
    26161 ctccgaagca gtgaaacaac ggcacgcata tacaacattt gtcatcgggg gtaaacttca
    26221 tgtgtactcc ggtgaggtaa ccgttgttca ctatgttgta atgtgtgagc ataactcctt
    26281 tcggaaatcc ggttgtgccc gaggtatatt gcatattgac cacatcctca cagcaagtca
    26341 aagctcgaag ccgtttgaat tcatcgtcat ccacgttatt ccccaacagc agaatttcgg
    26401 ccgtgttgta cattccccga tacttttcct gcccgatata aaccacgttt ttcagttccg
    26461 gaaagcgttc gcttctgaac ttgccgcgtt gagtggtttt cagttcgggc accagattgt
    26521 aaataatatc cgtataattg tttccggcag ctccgtcggt catgcaaagg gtgtgcatat
    26581 cagaattctt gagcagatag gacacttctt cggatttgta gcttgtgttt accgtgacag
    26641 caacagcacc gattttggcg gtagcataga ggaaagtcag ccagtcgggt acattttgcg
    26701 cccatatccc tacattcgtt ccgcgggtta ccccaattgc tatcagccct ttggccatat
    26761 tgtccacacg ttcgttgaag gttttccagc tgaaacgcag gttgcggtcg gaataaacga
    26821 tatattcctt atcgggcgat tcggctgccc agtactcaag ccagtcgctc agtgttctgt
    26881 cggaaagatg cattaattat taagtttaaa tgtttggttg tttggttgtt tggttgtttg
    26941 gttgtttgaa tgtttggttg cattgtgcag atttgaattc agaaaaagac tcagaaattc
    27001 gtattcattc gtgtaattcg ccatcattcg ccataattcg ttttaattcg tgtaattcgt
    27061 tcttatcaga aaggagaata tacaatcccg agaatttgtg ccggttcgtc ggtggcagtg
    27121 gtcaccaggt gatctacaat ggagtcgtag taaatgctgt cgccctggta gaggatgaag
    27181 ttatcgcggc cgtattttac cagtacagat ccgctcagca caaacaggaa ttcctctcct
    27241 tcgtgcgagg aggacacttt ttcgcctccg gccgacggct tgaggtcgat aagaaacggc
    27301 tccatgtgac gacctgcctt gcgcgatgcc agcgagaaaa aatccatgtg cgaattggct
    27361 gtggatagct ggctggagaa agtggctgcc tgttggtgat cggcactgcg gtttactacc
    27421 ggtccgaagt tctcgctgtc gtccagaaat gtacccagcc ttacacccag cgcccgcgca
    27481 atttttatca gtgacgacag tgaaggaata ttatcaccgg cgattatcat ttttatctgc
    27541 tctggagaaa gtcctgtgcg ttcggccagt tcctcttcag aaatgcccgt atcggtacag
    27601 aatgttttaa ttttgtttgt cacctcgttc atataaattt ctgtttttga ttacaacgtt
    27661 ttttatggtt tacagggcgc aaaagtatat atttttcaca ttacaggcaa attttattac
    27721 aaaatgtttg aattatactt gatttaattt tgatttaatc acagaagcag acatttaagc
    27781 attccggaca attgattttc ggaaagaagt taaaatagaa ttatgcaagt aaaattcatt
    27841 acttttgcat atcagaaaca gcaaaacaac aaatctgaac tatgaaacaa cgattcacct
    27901 tctttttact ctttgcggga attcttttca cttccgcaca aaagccctct caacaatggc
    27961 tcgaccagaa attttccatg tttatccact tcggacttta ctccgtttac ggtggtgtgt
    28021 atgatggaaa acctgtaacg agaggttaca gcgaacagat tcagtcgttt gccggtattt
    28081 tcagcgactg gtatggcaac actgcgcgtc ggttcaatcc ggtaaagtgg aatcccgaca
    28141 gcattgtaga tcttgccaaa aaagcaggca tgcgctccat tgttttcaca tcgaagcatc
    28201 acgacggatt ttgcatgtac cattcgaaac acaccggttt caatatcgtg gatgcgacac
    28261 cttacaagcg agatttaatg aaagaacttg cagaagcctg caaacgtggc ggaatcgatt
    28321 ttgcggttta tttttcgctg attgactgga attttccgca ggcatacccc atttcgagcc
    28381 acaatgccga tccgctcacg cctgaacatt atcaattcaa tctgaaacag gtggaagaaa
    28441 tcatga
//
LOCUS       DAORKP010000008        27213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_45_length_27213_cov_9.082554, whole genome shotgun sequence.
ACCESSION   DAORKP010000008 DAORKP010000000
VERSION     DAORKP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 27213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_27213_cov_9.082554"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2321)
                     /locus_tag="PLN75_01070"
     CDS             complement(45..2321)
                     /locus_tag="PLN75_01070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRG02738.1"
                     /translation="MRRIFIFLPLFCSFFTFANAQTPIDFVSTLVGTQSKHSLSTGNT
                     YPAIALPWGMNFWTPQTGKMGDGWVYAYDADKIRGFKQTHQPSPWMNDYGQFSIMPMT
                     GKPIFDEEKRASWFSHKAEIAKPHYYRVYLADYDVVTEIVPTERAAMFRFTFPQNANS
                     YVLLDAFDKGSYVKIIPNENKIMGFTTKNSGGVPDNFINYFVIKFDKSFTYKSVVENG
                     EIKNNDLEANANHAGAIIGFSTQRGEKVHVRVASSFISYEQAELNLKELGNRSFDQIK
                     EHGEKRWNEILGKIEVETTNIDQLRTFYSSLYRSVLFPRSFFEYDKEGNIVHYSPYNG
                     KVLPGYMFTDTGFWDTFRGLFPLLNLLYPEMNRKMQEGLINAYKESGFFPEWASPGHR
                     PVMVGNNSASVLADAYLQGNKVKEPDVLWEGIVKTANSVHPKVSSTGRLGYEYYNRLG
                     YVPCDVGIRENAARTLEYAYDDWCIYQLGKALGKPEKEIEVYAKRSQNYRNLFNKKYN
                     LMVGKMENGAFSEPFSPFKWGGDFTEGNSLHYTWSVFHDPQGLIELMGGKKTFNQMLD
                     TVFSLPPVFDDSYYGFTIHEIREMQIMNMGNYAHGNQPIQHMIYLYNYSGAPWKAQFH
                     LREVMDKMYTSAPDGYCGDEDNGQTSAWYVFSALGFYPVCPGSGQYVLGSPIFPKATI
                     QLENGKKVEITSVNNKKENVYVKALKVNGKNYTRNFLEINTLKKGMKLNFRMDNEPDF
                     TRGTKQSDEPYSFSNQKY"
     gene            complement(2532..3734)
                     /locus_tag="PLN75_01075"
     CDS             complement(2532..3734)
                     /locus_tag="PLN75_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRG02739.1"
                     /translation="MGKVLVIGAGGVGTVVVHKIAQNPDVFTEVMLASRTKSKCDAIA
                     ADIKKRYHIDIKTAQVDADQVDELVALLKSFQPEILINVALPYQDLTIMEACLQAGVN
                     YLDTANYEPKDEAKYEYSWQWAYHDKFKKAGLTAILGCGFDPGVTSVFTAYAAKQHFD
                     EIHFLDIVDCNAGDHGKPFATNFNPEINIREVTQKGKYYENGKWIETEPHAVHKPLNY
                     PEIGAKESYVIYHEELESLVKNFPTIKRARFWMTFGQEYLTHLRVIQNIGMARIDEID
                     YNGCKIVPIQFLKAVLPNPGDLGENYKGKTSIGCRIKGFKNGNVKTYYIYNNCSHEAA
                     YKETGTQAVSYTTGVPAMIGAMMFLTGEWSKPGVYNVEQFNPNPFMEQLNKQGLPWKE
                     LHDVDLEL"
     gene            complement(3890..5020)
                     /gene="nspC"
                     /locus_tag="PLN75_01080"
     CDS             complement(3890..5020)
                     /gene="nspC"
                     /locus_tag="PLN75_01080"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRG02740.1"
                     /translation="MDYSKIPSPCYVIEEQSFRRNLALISSVAQRAEVEIILAFKAFA
                     MWSVFPVVREYVQCSTASSLSEARLAYEEMGSKAHTYAPAYSEAEFPEIMSLSSHITF
                     NSLSQFQKFYPYTLGKNISCGLRINPEYSDVETDLYNPCAPGSRLGVVADLLGDHLPE
                     GVEGLHFHTLCESNSFDLEKTLAVIDEKFGKYFPQIKWLNMGGGHLMTHKDYDVEHLI
                     TLLKNFRAKYPHLHIILEPGSAFAWQTGFLLSTVLDVVENKGIKTAMLDVSFSCHMPD
                     CLEMPYKPAIRGASDVISGKPAYRMGGNSCLSGDFYGSWSFENELKVGDQVIFEDMIH
                     YTMVKTTMFNGVTHPSIGILTKENEFKLIRRFGYEDYKNRIS"
     gene            complement(5078..6703)
                     /locus_tag="PLN75_01085"
     CDS             complement(5078..6703)
                     /locus_tag="PLN75_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG02741.1"
                     /translation="MYFIAFSLSSQTKDEKYFKINKNLSVFNSILRELDAYYVDTLDY
                     DKVVLTGINSMLSSLDPYTVYMPEEMNDDLKMMTTGEYAGIGALIMQKDGRMVISEPY
                     EGMPAQKNGLRAGDVILEVDGVSATGKTTAQVSEMLKGKNGTEITIKIERWNEKKPIV
                     KKFIRENIQFNPVTYYAVLGNGVGYLMLNDFTDKAALEFKNAVNDMMKTGQIKSLIVD
                     VRNNPGGLVDEAVKIMGYFVPKGTSIVSTKGRNFETDRTYKTPSDPVFPEMKLAVMVN
                     RGSASASEILAGALQDLDRGIVIGERTFGKGLVQSIRPVGYGGNIKVTMAKYYTPSGR
                     CVQALDYSHRNEDGSVGRIPDSLTTAFKTANGRIVRDGGGVLPDSVTTDSRKLNIAYY
                     ILMQNHYFDYATRYAQKHKSIASPDKFSLTDAEFNDFVNYLVNEKKFTYTTQTENYFK
                     QLAEVAEFEGFDQTAKAEFDALKARLMPDVEKNIRDNRKEIEEMLTVEIIQRYYFQKG
                     QVQYMLKDDTDLKVATDFLKNNSRYNQVLKKQG"
     gene            complement(6889..7353)
                     /gene="coaD"
                     /locus_tag="PLN75_01090"
     CDS             complement(6889..7353)
                     /gene="coaD"
                     /locus_tag="PLN75_01090"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRG02742.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVMRGLNLFDQIIIGIGMNQSKKTL
                     FDMDNRVNMIRQAFKNEPRVSVETYDSLTIDFADAMGAKYILRGLRTVSDFEYEHTIA
                     NANRQLNGLETVLLFTETEYSFISSTVVRDLIRYGRDISGFLPPGVTCPPIP"
     gene            7680..9122
                     /locus_tag="PLN75_01095"
     CDS             7680..9122
                     /locus_tag="PLN75_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRG02743.1"
                     /translation="MQEKLQDKIFILISETADEMGLETYVIGGFVRDLFLHRPSKDID
                     IVTVGSGIALAEKVYKKLGKKAKFSFFKTYGTAQIKTHDWEVEFVGARKESYRQDSRN
                     PIVEDGTLEDDQNRRDFTINALALCLNQNRYGELVDPFNGLEDLKNFVIRTPLNPDIT
                     FSDDPLRMMRGIRFASQLRFFLEERTFDAITRNKDRIKIITKERVADELNKIILSPKP
                     SVGFILLEKTGLLQLIFPELLALKGAETKDGVGHKDNFFHTLAVLDELATRSDNLWLR
                     WSALLHDIGKPRTKKFDPKMGWTFHNHNFIGEKMIPEIFSKMKLPANEKMKYVQKMVG
                     LHMRPIVLSEDEVTDSAVRRLLFEAGDDIDDLMLLCNADITSKNPEKVRRYRQNFQLV
                     SEKLKDIEEKDRVRNFQPPIDGKEIMETFALPACNTVGEIKMKIKDAILDGIIPNEYE
                     AAKEFMLKTGEEMGLKTGDLPFESDKKKRE"
     gene            complement(9198..10046)
                     /locus_tag="PLN75_01100"
     CDS             complement(9198..10046)
                     /locus_tag="PLN75_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRG02744.1"
                     /translation="MLFSESLPNVKSGSIVRLNVASKYAEAKTVDVWLPPGYSTENKY
                     SVVYMHDGQMLFDSTKTWNRKEWQVDEVAAELIKNGQIKDCIIAGVWNSNENRISEYM
                     PTKIFNQLPDSIRAVFSEKYCNKRGAQGDAYLQFLVHELKPLIDSRFSTLPDAGNTII
                     MGSSMGGLISAYAICEYPGVFGKAACMSTSWLNFIDYRISLAALQYLSEHLPDCAGHQ
                     IYMDYGSGEADWKYAVSQQYADYIIKSKGFDERGFMSKVFENAIHDEISWSRRLHVPL
                     EFLLKK"
     gene            complement(10140..12200)
                     /locus_tag="PLN75_01105"
     CDS             complement(10140..12200)
                     /locus_tag="PLN75_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRG02745.1"
                     /translation="MNIKLTKEKINLFTRIGLFVSVVVIIVLLFPKDKKFKYQYEIGK
                     PWTYELITASFDFPIYKTDEQLEADRKELLSAYTPYFQYDEEVAGRQKEKWLSDWSKK
                     NPEPPAYSAYVERKLNEIFKKGIISSGVYEQLMSNNRKNIVKVYKDRKTEIISTEDIY
                     TPKSAYEEIVMNKPDYISVQDLSSYNLNLYLIDNLRFDSITSQNVRDDMVKNLSLTSG
                     MVQTGEKIIDRGEIVSPVTYSILNSMKIETQKRNTAFKESGYVIVGELIIVIGLILLL
                     TLYLYLFRQNLFENLNNLLFIALMILFVIGLASIVLRFTNLGIYIVPFTMLPIIIRVF
                     FDSRTALFAHFITMLIISFMVDNPFTFIVLQIIAGMTAVSGLKDLTARSQLTQTAFYI
                     FISYSVMYLATELISEGAINRIYFMPLAYFAISSALLLLAYVLIYLFEKIFNLISAIT
                     LVELTNINSDLMIRFAENAPGTFQHSLQVSNLATEAAKKIGANSLLVRTGALYHDIGK
                     MINPQYFIENQVGASNPLLEMNYVQAAQAIIRHVPDGVILAKKHGLPDQIIGFITTHH
                     GRTKTKFFYNSFVNDNPGITPDEEKFTYPGPLPYSKETAILMMSDAVEARSRTLGEYT
                     EESISKMVDSMIDSQIADGQFKDAPISFKDVETVKKVLTEKIKNIYHSRIAYPELNKD
                     IAVK"
     gene            12356..13129
                     /locus_tag="PLN75_01110"
     CDS             12356..13129
                     /locus_tag="PLN75_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRG02746.1"
                     /translation="MFTIITMPLIPMRKEAGDQYEMVSQLLFGQQIEILETTDNWYFV
                     KNLSDGYEGWIGKKSLFPKHFTGEMTDTSGFIATRMPLVVCFKTSSVEKITLPGGSML
                     PRIDRDQFELLGEIYQIAQTEPVYTKSGHAQLIVELAHQYINAPYLWGGKSLFGMDCS
                     GLTQVVYSMAGIYLPRDASQQVELGTVVDFLSEVQPGDLAFFANSEGSIIHTGILINS
                     HQIIHASGCVKKEIIDSQGIISSQNGEYSHHLRVIKRLL"
     gene            13200..13709
                     /locus_tag="PLN75_01115"
     CDS             13200..13709
                     /locus_tag="PLN75_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRG02747.1"
                     /translation="MRKTLSILAILVFFTSAGQAQSLEKLFRKYSDDERFEYTSVGKG
                     MLGFAQIFADFSILTEGQMEKITGFKMLKLQKDSAYQELTDKFMNDIDAVIEKGDFET
                     SLVKREKDKRIYVYKRIDRRANADMLLLTKSNTAINFVWLKGKTSDEEADRATFEKEQ
                     RAKEEIEIL"
     gene            complement(13757..14386)
                     /locus_tag="PLN75_01120"
     CDS             complement(13757..14386)
                     /locus_tag="PLN75_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRG02748.1"
                     /translation="MLVDIFIKGIIIGLATSVPLGPIGMLAIQRTLNRGLKHGLFTGL
                     GGTVSDIVYSVIILFFLSFVIDTIEVHRHAIQIIGSVIIIFFGLWIFRSNPIAQPLPH
                     EKPVEHSLLNDFVSSFGLTLSNPLIIFVLIALFSHLNFVTAETSVTGQVLGLTAIFTG
                     SISWWIVLTTFVNRFRTRFNIRGLKIINRVTGSVIMSLGLFSTFTSFLK"
     gene            complement(14433..14657)
                     /locus_tag="PLN75_01125"
     CDS             complement(14433..14657)
                     /locus_tag="PLN75_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02749.1"
                     /translation="MKKLIAVILILLGIAATTLSIREYAGKKKNIELAGFELSLTKRE
                     SKKIFYRNTGIGLVMLVGGVALLRGRKRSR"
     gene            complement(14674..15996)
                     /locus_tag="PLN75_01130"
     CDS             complement(14674..15996)
                     /locus_tag="PLN75_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRG02750.1"
                     /translation="MKSKKNFVIDTNVILHDYNCLHNFEENDIYIPIVVLEELDHFKK
                     GNEQINFNAREFVRELDLIANDNLFKNGAELGEGKGKLFIVAGNGYPEAISAVFPEKT
                     PDHRILADTYNIGKLKKASKTILVSKDINLRMKALSLGILAEDYINDKVTDLNIFEKN
                     HEVYENVAPEMIDRMYAEGGIPLDEFDFKSDVEPQECFVLKSSRSSVLARYQPQTGRV
                     IRIEKTRSFGIEPRNAEQAFAMNVLLDDEVKLVALTGKSGTGKTLVALAAALQKNEHY
                     KQILLARPIVALSNKDLGYLPGDEQHKIAPYMQPLFDNLNVIKHNFSYQGKEMKLLDE
                     MQKNEQLVIEALAYIRGRSLNETFFIVDEAQNLTPHEVKTIITRAGEGTKVVFTGDIQ
                     QIDSPYLDMHSNGLVYMIDRMRGQDIFAHVNLVKGERSYLSELASNLL"
     gene            complement(16150..16488)
                     /locus_tag="PLN75_01135"
     CDS             complement(16150..16488)
                     /locus_tag="PLN75_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG02751.1"
                     /translation="MKKFTLILALILCAFTGVHALKVVNTAPSMKTESVYLQSSTSVV
                     DNDDAPKFTLTGKVLSARNLENGAQVDIYSVLGARVHSYVYKGNPETLNLSKGIYIVR
                     SGKFTQKIIL"
     gene            16728..18392
                     /locus_tag="PLN75_01140"
     CDS             16728..18392
                     /locus_tag="PLN75_01140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG02752.1"
                     /translation="MTIKRSPEYIFETSWEVCNMVGGIYTVLSTRANTLCNKYGDKLI
                     FIGPDVWKNSESPYFDPDKELLKDWKEHFTQQTGLNIRIGRWKIHGNPVTVLVDFTPL
                     LYRKDSIYGHIWEKYGVDSLHAYGDYDESSMFGYATGIVIENYYRFHELTQQNNVVAH
                     FNEWMTTFGAFHIQEAVPEIATIFTTHATSIGRSIAGNHKPLYDYLSEYNGDQMAFEL
                     NMISKHSTEKKAAHCVDCFTTVSKITGLECTQLLEKTPDVITPNGFEDDIVPTGNEYK
                     SKRAEARSRLKLVAESLFGYELKKDTLFVSTAGRYEFKNKGIDVFIESLKRLSQMSHF
                     NREVVAFIMVPAHIKGPRPDLAARLSNPDAEMDYWNRQTTHDLYDYQHDSVISSLRWF
                     HFYNLKNEKVKIIFVPSYLSGNDGIFNMTYWDLLIGMDISVFPSYYEPWGYTPLESVA
                     FSVPTITTDLSGFGQWMKDNFENKHGAAVVHRSDYNFSEVTDSIAQILNDFLNQNKKE
                     MDDARKRAFITSKKALWSEFIQYYYEAYSRALEKRNKRIKNFPTIK"
     gene            18542..21103
                     /gene="glgP"
                     /locus_tag="PLN75_01145"
     CDS             18542..21103
                     /gene="glgP"
                     /locus_tag="PLN75_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRG02753.1"
                     /translation="MKLKANEVNEPTWRDIVVKSNLPESLNKLEELSQNMWWAWNSDA
                     KKLFREIDRPTWKEAESNPVQLLNLLSYDKLVALGKDEEFTKKVDSVYNEFTAYMNEP
                     TDTKRPSIAYFSMEYGLSEILKIYSGGLGVLAGDYLKEASDSNIDMTAIGFLYRYGYF
                     KQSLSIDGQQISMYEPQNFNILPIKQVLDEQNLPFVVEVPYPGRTVYAHLWKVSVGRI
                     ALYLLDTDIDENHDYDRSITHQLYGGDWENRMKQEIMLGIGGILALNKLGIKKQIYHC
                     NEGHAALINVQRLVDLIQNEKLTFAQALEVVRASSLYTVHTPVPAGHDSFDESLFSKY
                     MSEYPAKLNISWADFIDMGRTNPGTNEKFNMSVFACHTSQEVNGVSWLHGEVSKQMFA
                     PIWKGYFPEESHVSYVTNGVHLPSWTSSEWKALYEKHFTKAFYEDQSNVKIWEKIYQV
                     PDQEIWNIRTSLKNKLVEYIRMQFRDTWLKNQGDPSRIVSILEAINPNSLIIGFGRRF
                     ATYKRAHLIFSDLERLEKIVNDPIHPVQFLYTGKAHPADGAGQGLIKRIVEISRMPQF
                     LGKIIFLENYDMRLAKRLISGVDIWLNTPTRPQEASGTSGEKASMNGVLNFSVLDGWW
                     YEGYRKNAGWALTDKRTYENQEYQDKLDAITIYNTLEDEIIPLYFAKNTKGYSPEWIQ
                     FIKNNIAQITPNYTTKRMMDDYFDRFYMKQAKRTAMLSANHFQTAKEIADWKEKMAEG
                     WNNIEVVALDLPEKLVQNPMAGEDYSINITLDVKQNNMKGIGVELVAIQTDQNNNSKL
                     SSVDELTLVKTDGTKLTFNISYQLDNAGAYKYGFRMFPKNDLLPHRQDFCYVRWI"
     gene            complement(21185..22213)
                     /gene="mltG"
                     /locus_tag="PLN75_01150"
     CDS             complement(21185..22213)
                     /gene="mltG"
                     /locus_tag="PLN75_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRG02754.1"
                     /translation="MLKSKVAKILILIALVAIIGFSYVAYLFLLPNVKLRTDKNEFLL
                     IRENTGFEEVLQQLQAKADVKNVTTFRQAAKYLRYKNVKSGRYKIENGMNNLTLVRNL
                     RNGTQTPVNLTFNNIRTKEQLAGRLASQLMPDSAEILELLNDTAFLAGYNLNPYTSVS
                     AFIPNTYEVFWNMSAKRMFERLYKEYDKFWTEERKRKAEAIPMTQAEVSTLASIVDAE
                     TNSKAEKPVVAGLYINRLRKNMPLQADPTVIFATGDFSLRRVLNVHTRIESPYNTYRN
                     TGLPPGPIRIPTISGLEAVLNYDRNDYIFMCAKETLNGEHNFAATWSQHTVNAARYQK
                     ALNERGIR"
     gene            22371..22457
                     /locus_tag="PLN75_01155"
     tRNA            22371..22457
                     /locus_tag="PLN75_01155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22405..22407,aa:Ser,seq:gct)
     gene            complement(22506..22949)
                     /locus_tag="PLN75_01160"
     CDS             complement(22506..22949)
                     /locus_tag="PLN75_01160"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRG02755.1"
                     /translation="MKKQLKIALVAHDARKADMVEWAVFNSDMLSQHKLICTGTTGGL
                     IQQAFEDEGIEVEVKRMNSGPMGGDAEIAALVVNKQIDFCVFLIDDLSANPHEADIQM
                     LLRQCRIHNVPIACNRYSADLMITSPLWDDEEYEPKAPQYVPFKR"
     gene            complement(23219..25345)
                     /gene="scpA"
                     /locus_tag="PLN75_01165"
     CDS             complement(23219..25345)
                     /gene="scpA"
                     /locus_tag="PLN75_01165"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRG02756.1"
                     /translation="MKPNFKEIDIQNFVASTVAPEAEGSWLTAEQIAVKPQYTKEDLA
                     GMEHLNYAAGIAPFLRGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLS
                     VAFDLATHRGYDADHERVVGDVGKAGVSICSVEDMKVLFDGIPLNQMSVSMTMNGAVL
                     PILAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPKFSMKIIADIFEYTSK
                     NMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRAGVNAGMDIDAFAPRLSFFW
                     AIGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVG
                     RTTIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQQETDITREVDPWA
                     GSYYVESLTNELAEKAWELIEEVEKLGGMAAAIETGIPKMRIEEAAARTQARIDSGAQ
                     KIIGVNEYRLEKEDPIDILEVDNTAVREQQIARLKELRTNRDNAAVQKALDAITECVR
                     TGEGNLLELAVEAARLRASLGEISDACEKIVGRYKATIRTISGVYSSETKNDSDFERA
                     SGLTEQFAKKEGRRPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAA
                     KQAVENDVHILGVSSLAAGHKTLVPQVMDELKKLGREDIIVIAGGVIPAQDYDYLYKA
                     GVAAIFGPGTSVTKAAIAIMEILLEE"
     gene            complement(25408..25956)
                     /locus_tag="PLN75_01170"
                     /pseudo
     CDS             complement(25408..25956)
                     /locus_tag="PLN75_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661054.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(26042..26716)
                     /locus_tag="PLN75_01175"
     CDS             complement(26042..26716)
                     /locus_tag="PLN75_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRG02757.1"
                     /translation="MVKQNFQKTDNPVQTFFKTPIFRSWGPILVAAVLWFLMFSIWTK
                     HLFNFWIGIAISASVLMVVAIILRKEILKQFQWTWKAIGFGLLAATALYIVFYLGEYF
                     SGLLFGFAREQVDNIYALKEGQSKLWIALGLIFIMGPAEEIFWRGYVQQNFINKYGEW
                     KGFVFATLIYALVHIWSFNFMLVMAALVCGIFWGLMYRYNKNLVPLVISHAVWDVAVF
                     ILLPIM"
     gene            complement(26786..>27213)
                     /locus_tag="PLN75_01180"
     CDS             complement(26786..>27213)
                     /locus_tag="PLN75_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRG02758.1"
                     /translation="YLVMTNALSWKMLLVAAAPGFLIVNVLHANNTRDIKHDGVAKIK
                     TAAMLLGIKGSIIYYFAFTIGAYLLIVLCVIFGIQHWATLLIFISLPMALKLMKQMRT
                     ADKEKPENIQKLDEGTAQLVMVYMLLLVIGNFAGGFLNA"
BASE COUNT         7812 a         5864 c         5520 g         8017 t
ORIGIN      
        1 ttatccttgt tggatttctt acggcataat gagctatttt ttccttaata tttttgattt
       61 gaaaacgaat acggttcatc actttgtttt gtgccgcgtg taaaatctgg ttcattgtcc
      121 atccggaaat tcagtttcat tcctttcttc aacgtgttaa tttccagaaa attgcgggtg
      181 tagttcttgc cattcacttt caaagctttc acatacacat tttctttttt attattgaca
      241 gaagtaattt ccactttttt gccattttcc agttgaatgg tggcttttgg gaaaatgggc
      301 gagcccagca cgtattgtcc gctaccggga caaacgggat aaaaccccag ggccgaaaaa
      361 acataccagg ctgaagtttg accgttatcc tcgtcgccac aatagccgtc aggtgcagat
      421 gtgtacatct tgtccattac ttccctgagg tgaaattgtg ctttccaggg cgctccggaa
      481 taattgtaaa gataaatcat gtgttggatg ggttggttgc cgtgggcgta atttcccata
      541 ttcattatct gcatttcgcg gatttcatga atggtaaagc catagtaact atcgtcaaat
      601 acaggcggca acgagaatac ggtatccagc atctgattaa atgttttctt tcctcccatc
      661 aactcaatca agccctgcgg atcgtggaaa acagaccagg tgtaatggag cgaatttcct
      721 tcggtaaaat cgccgcccca tttgaaggga ctgaaaggct cactgaatgc tccgttttcc
      781 atcttcccta ccatcaggtt gtatttttta ttgaaaagat tccgataatt ctgactgcgt
      841 ttggcataca cttcaatttc tttttcgggt tttcccaacg ctttaccgag ttgataaata
      901 caccagtcgt cgtatgcata ttccagtgtt cgtgcggcat tttccctgat acccacatca
      961 cagggtacat atcccagtcg attgtaatat tcgtagccca accttccggt ggatgatact
     1021 ttcgggtgaa cactgtttgc agttttcaca attccttccc aaagtacatc cggttctttc
     1081 actttattgc cctgcaaata agcatcggct aaaaccgaag cagagttatt cccaaccatg
     1141 acaggtctgt gaccgggact tgcccattcg gggaaaaacc cgctttcctt gtaagcattg
     1201 atcaatcctt cctgcatttt tcggttcatt tcgggatata gcaggttcaa aagtggaaac
     1261 aggccacgga atgtatccca aaatccggta tcggtgaaca tgtatccggg caacactttg
     1321 ccgttgtagg gactgtaatg cacaatgttt ccctctttgt catattcaaa aaaactccgc
     1381 ggaaaaagca ccgaacgata aaggctggag taaaaagtgc gtaactggtc gatattggta
     1441 gtttcaactt ctatcttgcc gagaatttcg ttccatcgtt tttctccgtg ctcttttatt
     1501 tggtcgaaac ttcgattgcc gagttctttc aaattcaatt ctgcctgttc gtagcttatg
     1561 aatgatgaag ccacccggac atgaaccttt tcgcctcttt gggtggaaaa accgattatt
     1621 gcacctgcat ggtttgcgtt tgcttcaagg tcgttatttt ttatttcgcc gttttcgact
     1681 accgatttat aagtgaatga tttatcgaat tttattacaa agtaatttat aaaattatcg
     1741 ggcactcctc cgctattttt agtagtaaaa cccataattt tattttcgtt tggaataatt
     1801 ttgacatacg agcctttatc aaaagcatct aataaaacgt atgaattagc gttttgagga
     1861 aatgtaaacc ggaacatggc ggctctttct gtaggcacaa tctcggtaac aacatcgtaa
     1921 tcagcaagat agactctata ataatgtggc ttagctattt ctgccttgtg tgaaaaccag
     1981 cttgcccgct tttcttcgtc gaatatgggt tttccggtca tcggcataat cgaaaactgg
     2041 ccgtaatcat tcatccacgg acttggctga tgagtttgtt tgaaaccgcg aattttgtcg
     2101 gcatcgtagg cataaaccca tccatcgccc atttttccgg tttgcggtgt ccagaaattc
     2161 attccccatg gcagtgctat agccggataa gtattgccgg tggaaaggga atgtttggat
     2221 tgagtaccaa ctaatgtgct gacaaaatca atgggtgtct gtgcgttggc aaaagtaaaa
     2281 aatgaacaaa aaagaggtaa aaagatgaat atccttctca ttttctttca atttgaagat
     2341 tgttaaggta aaaaataact ctaataagtg catttaaatt gaataaattc aacgatcagc
     2401 ataaagtgct tgaaattact gctaaattat ggattctgca agtcgttcat acattcctga
     2461 gaaaggatgg taaaaaaata aggataccga tttctcaata tccttattat caattattta
     2521 ttatttttgg atcataattc cagatccaca tcatgcagct ctttccacgg aagtccctgt
     2581 ttgttaagtt gttccatgaa tggattggga ttaaactgtt caacgttgta tacacccggt
     2641 ttgctccatt cacctgtgag aaacatcatg gcgccaatca tggcgggcac tccggtggtg
     2701 tagcttacag cttgtgtgcc ggtttccttg taggcggctt catgactgca attgttgtaa
     2761 atatagtatg tctttacgtt accgtttttg aatcctttga tgcggcagcc gatggaggtc
     2821 ttgcctttgt aattttcgcc caggtcaccg gggttgggta aaacggcttt cagaaactga
     2881 atgggcacta ttttgcatcc gttgtaatct atttcgtcga tgcgcgccat gccgatattc
     2941 tgaattacac gcagatgtgt gaggtattcc tgcccgaaag tcatccaaaa acgtgccctt
     3001 ttgatggtcg ggaagttttt caccagcgat tccagttctt cgtgataaat cacatacgat
     3061 tctttggcac cgatttccgg ataattcaaa ggtttgtgta cagcgtgtgg ctctgtttca
     3121 atccatttgc cgttttcata atatttacct ttctgggtca cctcacggat gttgatttcg
     3181 ggattgaagt ttgtggcaaa aggtttgccg tggtcacctg cattacaatc cacaatatcc
     3241 agaaaatgta tttcgtcaaa atgttgtttg gcggcgtaag cagtgaaaac gctggttaca
     3301 cccgggtcga atccgcatcc aagaatagcg gtaagtccgg cttttttgaa tttatcgtga
     3361 tatgcccact gccagctgta ttcgtattta gcttcatcct ttggttcgta gttggctgta
     3421 tccaggtaat tgacgcctgc ttgcagacaa gcctccataa tggtcagatc ctgatacggc
     3481 aatgccacgt tgataaggat ttcaggctga aaacttttca gcaatgctac cagttcgtca
     3541 acctgatcgg catctacctg agctgttttt atatcaatat gataccgttt tttaatgtca
     3601 gcggctattg catcgcattt tgattttgtg cgacttgcca gcattacttc tgtaaacaca
     3661 tccggatttt gagcaatttt atgaacgact acagtcccta cacctccggc acctatgact
     3721 aatacttttc ccatttttta ctttttaaaa aagtcggttt atttaatttg ttgaacaaaa
     3781 atgaaacctc attcctgctc tttttttgaa cacacaaaag taaatcaata aaacttaaac
     3841 accaaattaa ttttagtttt tacacaaaaa cgaattaaac aactaattat caagatattc
     3901 tgtttttata atcttcataa ccaaatctcc ggatcagttt gaactcattt tctttggtca
     3961 gaataccgat ggagggatgt gtcaccccgt tgaacatagt ggtttttacc atcgtataat
     4021 gtatcatgtc ttcaaatatt acctgatcac ctaccttcag ttcgttttca aacgaccagg
     4081 aaccataaaa atcacccgac aggcaactgt tgcctcccat gcggtaagcc ggttttccgg
     4141 atatgacatc cgaggctcct ctgatggccg gtttataggg catttcgagg caatcgggca
     4201 tgtggcacga gaatgataca tcgagcatgg cagtcttaat tcctttattt tcaacgacat
     4261 ccaaaacagt ggagagcaaa aatccggtct gccacgcaaa tgcacttccg ggctccagaa
     4321 taatatgcaa gtgcggatat tttgccctga aatttttcag caaagtaatc aaatgttcca
     4381 catcgtaatc cttatgtgtc atcaggtggc cacctcccat gttgagccac ttaatttgcg
     4441 ggaaatattt cccgaatttt tcatctataa cggccagtgt tttctccaga tcaaatgaat
     4501 tggattcaca aagtgtatga aaatgaagcc cttctacccc ttcgggtaaa tgatcgccca
     4561 gcaaatcggc caccactccc aatcgggaac cgggagcaca gggattgtat aaatcggttt
     4621 cgacgtccga atattcagga tttatcctca gtccgcagga tatattttta ccaagtgtgt
     4681 acggatagaa tttctgaaac tgcgacaaag aattgaatgt aatgtggctg ctcagactca
     4741 ttatttcagg gaattctgct tcggaatacg caggtgcata cgtgtgagct ttgcttccca
     4801 tctcctcata agccagacgt gcttcggaga gcgaactggc tgtactgcac tgcacatatt
     4861 cacgtacaac agggaaaaca gaccacatag cgaatgcctt gaatgcaaga ataatttcga
     4921 cttcagcacg ttgggcaaca gagctaatta gtgctaaatt ccggcgaaat gactgctctt
     4981 caatcacata gcagggtgaa ggtatttttg aataatccaa ctttgtaaag agtttaatgg
     5041 aaatttataa tatcggattc ggtttttaaa aaacatgtta tccctgtttt ttcaacacct
     5101 gattgtacct gctgttgttt ttaagaaaat cggtggccac tttcaggtca gtgtcatcct
     5161 tgagcatgta ctgcacctga cctttctgga aatagtatct ttgaataatt tccacggtga
     5221 gcatttcctc tatttccttt ctgttgtcgc ggatattttt ttccacatcg ggcatcagtc
     5281 tggctttcag agcgtcaaat tcggctttgg ctgtttggtc aaaaccttca aattcagcta
     5341 cttcggcaag ttgtttgaaa taattttcgg tttgagtagt gtatgtaaat ttcttttcgt
     5401 tgaccagata gtttacgaaa tcgttgaatt cggcatctgt cagactgaat ttatcgggtg
     5461 aagctatact tttgtgtttt tgagcgtacc gggtggcata atcaaaatag tggttctgca
     5521 taagaatgta ataggcaatg ttcagtttgc gggaatcggt agtcacgctg tcgggtaaaa
     5581 ctccaccacc gtctcttact attcgtccgt tagcggtttt gaaagcggtg gtcagactgt
     5641 cgggaatacg tccgacgctt ccatcctcgt ttcgatggct gtaatccagc gcctgtacgc
     5701 atcttccgct aggtgtgtaa tacttagcca tggtcacttt tatatttcca ccataaccaa
     5761 ccggtcgaat gctctgtacc agtcctttgc cgaatgtacg ctccccgatt acaattccac
     5821 ggtcgagatc ctggagggct ccggccagaa tttccgaagc tgatgccgag cctctgttga
     5881 ccatcacggc aagtttcatt tccggaaaaa cagggtcgga aggtgtctta taagtgcgat
     5941 cggtttcgaa attacggcct tttgtagata caatggatgt tcctttcggc acaaaatagc
     6001 ccataatctt cactgcttcg tcgaccaaac cgcccggatt gtttctcaca tccacaatca
     6061 gcgattttat ctgaccggtt ttcatcatgt cgtttacagc atttttgaat tcaagggcgg
     6121 ctttatcggt gaaatcattg agcatcaggt aacccacacc atttccgagc accgcataat
     6181 aagttaccgg attgaactgg atattttcac gtataaattt cttcacgatt ggttttttct
     6241 cgttccagcg ttctatttta atcgtaattt ctgttccgtt ttttcctttc agcatctcac
     6301 ttacctgagc agtagttttg ccggttgcac ttacaccgtc cacttccaga attacatctc
     6361 cggcgcgaag tccgtttttc tgtgcgggca taccttcata cggctccgaa atgaccatgc
     6421 ggccgtcttt ctgcataatc agagctccaa ttccggcata ttcaccggtg gtcatcattt
     6481 tcagatcatc gttcatttcc tcaggcatgt aaaccgtata aggatcaagc gacgaaagca
     6541 tgctgttgat acccgtgagc actactttgt cgtagtccag tgtatccaca tagtaagcat
     6601 ccaactctcg tagtatgcta ttgaatacac tcaggttctt atttattttg aaatactttt
     6661 catctttggt ttgcgaagag agcgaaaatg caatgaaata caaaagaaaa atgttcgcta
     6721 ataccctgta ggaggtatat ccaggttcaa tattaagttt tctgaaatga attgacattt
     6781 tatatatttt cttttaatgt gaattgactc attttataat agaaatgtaa acaaaaaaat
     6841 gtgtttagca gcgttaagtg cttaaagaca aactcaaatc actattcctc agggaattgg
     6901 cggacaggtt accccgggag gtaagaagcc gctgatatct ctgccgtacc ttatcaaatc
     6961 gcgtaccaca gtggatgaaa taaatgaata ttctgtttct gtgaatagca gtacggtctc
     7021 cagaccattc agttgacggt tggcattcgc aattgtatgt tcgtactcaa aatcggaaac
     7081 tgtgcgcaaa cctcgcaaaa tatacttcgc tcccatagca tccgcaaaat caatggtcag
     7141 gctgtcatag gtctctacag atacccgcgg ttcgttctta aaagcctgac gaatcatatt
     7201 caccctgttg tccatgtcaa acagtgtttt tttggactga ttcataccga tgccgattat
     7261 tatttgatca aaaaggttca aacctcgcat tacaatgctg taatgcccga tggtgaacgg
     7321 atcgaaagta cctggaaaaa tggctcgttt cattttattt tcagctatct gacgccggaa
     7381 ttatcgattt caggctaaca atgaaattct atgtactgaa aatctgatat ctgacgtctg
     7441 tttaagaata caaagatacg taaaatttta acgttactga tttcagacgg tgtcgttttg
     7501 gttttttaaa ggttggtgaa caacaatttt tattatgttc aaagttaata aggatattct
     7561 tcaatccttc aattcggagg ataattttac ccgtacacgc tatttttttt cattctgtca
     7621 ctctttttgt tcattccccc aaaaaccact aattttgtat tacgaaaaaa aacaattaca
     7681 tgcaggaaaa actacaggat aaaattttca tactgatatc agaaactgcg gatgaaatgg
     7741 gattagaaac gtatgtcatc ggtggatttg tcagagatct gttcctccat cgaccgtcga
     7801 aagacattga catagtgaca gttgggagcg gaatagctct ggctgagaag gtttataaaa
     7861 aactgggcaa aaaagcaaaa ttctcctttt tcaaaaccta cggcaccgct caaatcaaaa
     7921 cccacgactg ggaagtggaa tttgtaggtg cccgcaagga atcgtacagg caggattcac
     7981 gcaatccgat tgtggaagac ggcacgctgg aagacgacca gaaccgccgt gacttcacca
     8041 tcaatgcgct cgctctgtgt ctgaatcaaa accggtacgg tgaactcgtc gatccgttca
     8101 acggactgga agacctcaaa aactttgtca tacgcactcc cctcaatccc gatattactt
     8161 tctccgacga tccgctgcgt atgatgcggg gcatccggtt cgcgtcacag ctccggtttt
     8221 ttctcgaaga gagaacattt gatgctatta cacgaaacaa agaccggatc aaaatcatta
     8281 ccaaagagcg tgtagccgac gaattgaata aaatcatcct gtcgcctaaa ccgtctgtcg
     8341 ggtttattct actggaaaaa acaggattac tgcaacttat ttttccggaa ctgcttgcgc
     8401 tgaaaggcgc cgaaaccaaa gacggtgtag gacacaaaga caattttttc catacgcttg
     8461 ccgtgctcga tgaacttgcc accaggtcgg ataacctctg gctgcggtgg tcggctctgc
     8521 tccacgatat cgggaaaccg agaacaaaga aatttgatcc gaaaatgggc tggactttcc
     8581 acaaccataa tttcatcggc gaaaagatga tccctgagat tttcagcaaa atgaagctgc
     8641 ctgcgaatga aaaaatgaag tatgtgcaaa aaatggtcgg actgcacatg cgtccaatcg
     8701 tgctgagcga agatgaagtg accgattcgg ccgtacgtcg gctgcttttt gaagccggtg
     8761 atgatatcga tgatctgatg cttctttgca acgcggatat cacgtccaaa aatccggaaa
     8821 aagtacgccg gtatcgtcag aattttcagc tggtaagcga gaaactgaaa gatattgaag
     8881 aaaaagaccg tgtgcgcaat tttcagccac ccattgacgg taaagaaatc atggaaactt
     8941 ttgccctgcc tgcctgcaat acagtcggtg aaattaaaat gaaaattaaa gatgccattc
     9001 tggatggcat tatccccaat gagtacgaag ctgctaaaga attcatgcta aagacaggcg
     9061 aagaaatggg attgaaaaca ggtgatttgc ccttcgaatc tgacaaaaaa aaacgggaat
     9121 aagtcaggct gatttaccaa agttcacacg acagattaca tgctgaaagg aaggtttttt
     9181 catgaaactt tcctttttta ttttttcaac agaaactcaa gaggcacatg taatcgtctg
     9241 ctccatgaaa tttcgtcgtg aatggcgttt tcaaacactt tcgacataaa accgcgctcg
     9301 tcaaatcctt ttgatttgat tatgtaatct gcatattgct gacttacagc atatttccag
     9361 tctgcctcac ccgaaccgta atccatgtaa atctgatgac cggcgcaatc gggtaaatgt
     9421 tcgctcagat actgtaaagc agccagcgaa atccgataat cgataaaatt caaccacgaa
     9481 gttgacatgc aggctgcctt tccgaaaacc ccgggatact cgcagatagc ataggcagaa
     9541 atgagcccgc ccatacttga gcccatgata attgtattgc cggcatcagg cagagtcgaa
     9601 aaccgtgaat caatcaacgg cttcagttcg tgtaccagaa attgcaggta agcatcaccc
     9661 tgagcacccc gcttgttgca atatttttca gaaaaaacag cccggattga atcaggcagc
     9721 tgattgaata ttttagtggg catatattcc gaaatgcggt tttcattgct gttccacact
     9781 ccagctataa tacagtcttt tatctgcccg tttttgatca gttccgccgc aacttcatcc
     9841 acctgccatt ctttccggtt ccaggtttta gtgctgtcaa aaagcatttg accgtcgtgc
     9901 atatatacca cactgtattt attttcggtt gaataaccgg gtggtaacca gacatcaact
     9961 gttttggcct ctgcatattt cgaagccaca ttcaaccgta cgatactgcc tgatttcacg
    10021 ttcggaagcg attcagaaaa aagcatattt gaatttatca aaaaacagaa caggagaaat
    10081 gattttgtaa ttgtattcat ttgatagtaa gttatttaca cgcaacaaaa tatttggtat
    10141 tattttactg caatatcctt attcagttct gggtaggcaa ttcgtgagtg gtaaatattt
    10201 ttgatttttt cggtaagtac ttttttcacg gtttccacat ccttgaaact gatgggtgca
    10261 tctttgaact gtccgtcagc tatttgcgag tcaatcatgc tgtccaccat tttgctgatg
    10321 ctttcttctg tatattcgcc cagcgtgcgc gagcgtgcct ccactgcatc cgacatcatc
    10381 agaatggcag tttccttgct gtatggaagc ggaccggggt aggtaaactt ttcttcgtcg
    10441 ggtgttattc cgggattatc attaacgaat gaattgtaga aaaatttagt tttagtacgc
    10501 ccatgatgtg tggtgataaa tccaataatc tgatcgggca atccatgttt tttggccaga
    10561 atgacgccat caggcacatg tctgattatg gcctgtgctg cctgcacgta gttcatttcg
    10621 agcagcggat tggatgctcc cacctgattt tcaataaaat actgcggatt aatcattttc
    10681 ccgatatcgt gatacaatgc gccggtacga accagtaaac tgttagcacc tattttcttg
    10741 gctgcttcgg tggcaagatt ggacacctga agtgaatgtt ggaaagtgcc cggagcgttt
    10801 tctgcaaacc gaatcatcag atcactgttg atattcgtca gttcgaccag cgtaatggcc
    10861 gaaatgaggt tgaaaatttt ttcaaataaa taaatcagca catatgccag cagcagcaaa
    10921 gccgaactga tggcaaaata tgccaggggc atgaaataaa tccggttgat agcaccttcg
    10981 gaaatcagtt cggtagccag atacatcacc gaatagctga taaagatata gaaggcggtc
    11041 tgagtaagct gcgatcgggc agtaagatct ttgagtccgg atactgcggt cattccagct
    11101 attatctgca atacgataaa tgtgaacgga ttatcaacca tgaatgatat tatcagcatg
    11161 gtgatgaaat gggcaaaaag agcagttcgc gaatcaaaaa atacccgaat gatgataggc
    11221 agcatcgtaa agggtacgat ataaataccg agattggtga atctgagcac aatggatgca
    11281 agcccgataa caaacaaaat catcagagcg atgaaaagca ggttgttcag attttcgaac
    11341 agattctgac ggaaaaggta taaatacaat gtaagcaata gaataagccc aataactata
    11401 ataagctctc ctacaattac ataacccgat tctttaaagg ctgtatttct tttttgggtt
    11461 tcgattttca tcgaattcag gattgaataa gtcacaggcg aaacaatttc acctctgtca
    11521 attatttttt cgcccgtctg caccatcccc gaagtcaacg aaagattttt caccatgtca
    11581 tctcttacgt tttgagaggt aatactgtca aaccgaagat tatcaatcag atacagattc
    11641 agattgtacg agctcaaatc ctggacactg atataatccg gcttattcat cactatttcc
    11701 tcataggccg acttgggtgt gtaaatatct tctgtggaaa taatttcagt tttgcggtcc
    11761 ttgtatactt ttacgatgtt tttccggttg ttgctcatca gctgctcata cacgcccgac
    11821 gaaataatgc ctttcttaaa tatctcgttc aattttcttt ccacataagc cgaataagcg
    11881 ggtggttccg gatttttttt gctccagtcg ctcagccatt tctccttttg ccggccggcc
    11941 acttcttcat cgtactgaaa ataaggagta tatgccgaca gtaattcttt tctgtcagct
    12001 tccagttgct catctgtttt atatatcgga aagtcgaaag aagcagtgat gagttcataa
    12061 gtccagggct tacctatctc atattggtac ttgaactttt tatccttggg aaataacaga
    12121 acaattatta ccactaccga aacaaaaagt ccgatacggg taaataagtt tattttttct
    12181 ttcgttaatt ttatattcat ctttgagttg ttggcgtgga tgattaagtt gtaataactt
    12241 acttcatccg aataaatcac acaaaactat aaaaaaaaca agaataataa ccgatttgtt
    12301 ttaagtttat ttatttttgt aaaaaataca ccttgtaaat caaataaaac ctgacatgtt
    12361 cactatcatt acaatgcccc tgatacccat gcgcaaagaa gccggagatc agtatgaaat
    12421 ggtctctcaa ttgctttttg ggcagcaaat tgagatcctg gaaacaacag ataactggta
    12481 ttttgtaaaa aatctcagcg acggctacga gggctggatt ggaaaaaaaa gtttatttcc
    12541 aaaacatttc accggagaaa tgacagatac atccggattc atagctacca gaatgccgct
    12601 tgtggtatgt tttaagacgt cgagtgtgga aaaaataacg ctgccgggcg gtagtatgct
    12661 gccccggatt gatcgtgatc agttcgaact gctcggagaa atctaccaga tagcgcagac
    12721 agaaccggtt tacaccaaaa gcggtcatgc tcagctcatc gtggaacttg cgcatcagta
    12781 tatcaatgca ccttacctct ggggcggcaa aagcctgttc ggcatggatt gctcgggtct
    12841 cacgcaggtg gtatattcga tggcaggcat ttatttgcca cgtgatgcct cgcagcaggt
    12901 agaactcggt acggtggttg attttctctc ggaagttcaa ccgggcgatc tggctttttt
    12961 cgcaaattcc gaaggaagca tcattcatac aggcatactc ataaattcac atcagattat
    13021 ccatgcatcc ggatgtgtaa aaaaggaaat aattgattca caaggcatta tctcttcgca
    13081 gaatggagag tacagccatc acctgagagt aatcaaaaga ttactgtgat tttggaacga
    13141 attttgataa tgtaattaag cagttgaatt tttcaaaaca attatttaaa aatggaatta
    13201 tgagaaagac attatcaatt ttggcaattc tggtgttttt cacgtcggca ggtcaggctc
    13261 agtcacttga aaaacttttc aggaaatact cggacgacga gcgtttcgag tacacttcag
    13321 tagggaaagg gatgcttggt tttgctcaga tttttgcgga tttcagcatc ctgaccgaag
    13381 gtcagatgga aaaaattacc ggtttcaaaa tgctgaaact tcagaaagac tccgcctatc
    13441 aggaactcac tgataaattc atgaatgata ttgatgccgt gattgaaaaa ggcgattttg
    13501 agacatcgct cgtaaaacgc gagaaggata aaagaatata tgtttataaa cggattgacc
    13561 gaagagccaa cgctgacatg cttctgctta ccaagagcaa tacagccata aattttgtgt
    13621 ggctcaaagg caaaacaagc gacgaagaag ccgacagagc cacttttgaa aaagagcagc
    13681 gtgccaagga agaaattgaa attctataaa tcaacacagc atttattcga gaccaaacga
    13741 tccgtttggt ctttttttat ttcaaaaaag acgtaaacgt gctgaaaagc ccaagcgaca
    13801 taattacaga acctgtgacc cggttaataa ttttcaaacc gcggatattg aaacgggttc
    13861 ggaatctgtt gacaaaggtc gtaagaacta tccaccacga aattgaacct gtaaaaattg
    13921 ctgtcaggcc cagcacttgt ccggtaaccg aagtttcggc cgttacaaaa ttcaggtgag
    13981 agaacaatgc tatcaacaca aagataatga gcggattaga caaggtgaga ccaaaagacg
    14041 aaacaaaatc attgagcagc gagtgctcta caggtttttc gtgaggtaac ggctgagcaa
    14101 tcggattgct tctgaaaatc cacaacccga aaaaaatgat gatcacgctg ccaataatct
    14161 gaattgcatg ccggtgaact tctatggtat caataacaaa acttaagaaa aaaagaatga
    14221 ttactgaata tacaatatca gaaaccgttc ctcccaaacc ggtaaacaga ccgtgtttga
    14281 gtccccggtt cagcgttcgc tgaatagcca gcatcccgat gggccccagc ggtactgaag
    14341 tagctaatcc gataattatc ccttttataa aaatatcaac gagcatttta tttttggctg
    14401 caattggttg ttggaatgtt ttctctgatt atttatctgc ttctttttct tcctctcagc
    14461 aatgcaacac ctccgacgag catgaccaat cctattccgg tatttcggta aaatattttc
    14521 ttactctccc tttttgtaag tgataattca aatccagcca gctcgatgtt ttttttcttg
    14581 cccgcatact ctctgatgct cagcgtagtg gcggctattc cgagtagtat caatatcact
    14641 gcaatgagtt ttttcatatc ttatgttgat tcattaaagt aaattacttg ccagctctga
    14701 cagatagctt ctttcacctt ttaccaggtt aacgtgggca aaaatatcct gtccgcgcat
    14761 gcggtcaatc atgtatacga gtccgttgga atgcatatcg agataaggtg agtcaatctg
    14821 ctgaatatcg ccggtgaata ccaccttggt gccttcacct gcgcgggtga tgattgtttt
    14881 cacttcatgt ggggtaagat tctgcgcttc gtccacaatg aaaaatgttt cgttcaggct
    14941 tcttccgcgg atataggcaa gcgcttcaat caccagctgt tcgtttttct gcatttcatc
    15001 gagcagtttc atctcttttc cctgatacga aaagttgtgt ttgattacat tcagattatc
    15061 gaataatggc tgcatgtatg gtgcaatttt gtgttgttcg tcgccgggga gataaccaag
    15121 atctttgttt gagagggcca caatgggtct tgccagaaga atttgcttgt aatgttcatt
    15181 tttttgcagg gcagcagcca gcgccaccag tgtttttccg gtacccgatt ttccggtcag
    15241 cgctacgagt ttcacttcat cgtccagaag cacattcatg gcaaaagcct gctcggcgtt
    15301 gcggggctcg ataccgaagc tgcgcgtttt ctcaatccgg atcaccctgc ctgtctgagg
    15361 ttgatatctt gccagaacgc ttgatctcga acttttcagc acaaaacatt cctgaggctc
    15421 cacatccgac ttgaaatcaa attcatccag cggaatcccg ccttcggcat acatgcggtc
    15481 aatcatttcg ggtgccacat tttcatacac ttcatgattt ttttcaaaaa tatttaggtc
    15541 tgttaccttg tcattaatat agtcttctgc cagaatacca agcgacaagg ctttcattct
    15601 cagattaata tctttactta caagaatagt tttggaggct ttcttcagtt tacctatgtt
    15661 atacgtgtcg gccagaatgc ggtggtcggg cgttttttcc gggaaaacgg ctgaaatagc
    15721 ttcaggataa ccgttgcccg caacgataaa aagttttcct ttgccttcgc ccagttcggc
    15781 tccgttttta aataaattat cgttggctat cagatccagt tcgcgaacga actcgcgtgc
    15841 attgaagtta atttgctcat tccctttttt gaaatggtcc aactcctcga gcactacaat
    15901 cgggatgtag atatcattct cttcaaaatt gtgtaaacag ttgtaatcgt gcaatatgac
    15961 gttggtgtct attacaaagt tctttttgct cttcatatcc taaaaaaata tttttttact
    16021 tttcataaaa aacacctgaa acaaccggat ggttcctact ctcaggtttt ttttacatag
    16081 ctcatgacaa gtttggcata aaaaaaccga caggcatact gtcggttccc gatatttttc
    16141 tctggcaaat tacagaatga ttttctgtgt aaatttacct gaacgaacga tgtaaattcc
    16201 tttgctcaaa ttcagagttt caggatttcc tttgtaaaca taagaatgaa ccctggcacc
    16261 caaaacactg taaatatcta cctgggcgcc gttttcaaga ttccttgcac tcaacacttt
    16321 acctgtgagg gtaaatttgg gggcatcatc attgtcaacc accgatgtac tgctttgcaa
    16381 atagacagat tcagttttca ttgaaggagc agtgttcacc actttcaggg catgtacacc
    16441 cgtaaatgca caaagtatca atgctaatat aagagtaaac ttcttcatag gcatgtaatt
    16501 aattgctgta ctttgtattt ttatctacaa atataatgca aaagtacaaa aaatgttctt
    16561 tgtttcacaa taatttctcg attaactcac tttattcaaa aaatttatat tcgacacaaa
    16621 aaattaacaa agattttata tatttgaaca aattcgattt gacggtttac caaatatccg
    16681 acaaaaaatg agtacctttg taactgattt ttttaaaaat taaacgaatg acaatcaagc
    16741 gctcacctga atatattttc gaaacaagtt gggaagtttg caacatggta gggggtatat
    16801 atacggtact ttccacacga gccaacacgc tatgcaataa atacggcgac aaactcattt
    16861 tcattggacc cgacgtatgg aaaaattccg aatcacccta ttttgatccc gataaagagc
    16921 tcctgaagga ctggaaagaa catttcacgc agcaaacagg gctgaatatc cgcatcggaa
    16981 gatggaaaat acacggaaat ccggtcacgg ttttagtgga cttcactccc ctgctctacc
    17041 gcaaggattc catctacggg catatatggg agaaatacgg tgtggattcg ctgcatgctt
    17101 acggcgatta cgacgaatcg tccatgtttg gatatgccac aggcattgtg atcgaaaatt
    17161 attaccgttt ccatgaactg actcaacaaa ataatgttgt tgctcatttc aacgaatgga
    17221 tgaccacctt tggagcattc cacattcagg aagccgtacc cgaaatagcc actattttca
    17281 ccactcatgc cacttccatc ggccggtcca ttgccggcaa ccacaaaccc ctttacgatt
    17341 atctgagtga atacaacggc gatcagatgg catttgaact caacatgatt tccaaacatt
    17401 ctacagaaaa aaaagcagct cattgcgtgg actgttttac cactgtaagt aaaattaccg
    17461 ggctcgaatg tacccaacta ctcgaaaaaa cacccgatgt gattactcca aacggctttg
    17521 aagatgatat tgtacctaca ggaaatgaat acaaatcgaa aagagccgaa gcccgttcga
    17581 gattgaaact ggtagccgaa agtcttttcg gatatgaact gaaaaaggac accttgtttg
    17641 taagtactgc aggcagatac gagtttaaga acaaaggcat cgatgtattc atcgaatcgc
    17701 tgaaaagatt atcacaaatg tcgcatttca atcgcgaagt tgttgcattt atcatggttc
    17761 cggcacatat caaaggtccc agacccgatc tggctgccag attgtccaat ccggatgccg
    17821 aaatggacta ctggaacaga cagaccacac acgatctata tgattatcag cacgatagcg
    17881 taataagctc tttgcgttgg tttcactttt acaacctgaa aaatgaaaag gtaaaaatta
    17941 tttttgtgcc ttcatacctg agcggaaacg acggtatttt caatatgacg tactgggacc
    18001 tgcttatcgg tatggatatc agcgtgtttc catcgtacta cgagccctgg ggatacacac
    18061 cgctcgaaag tgtggccttc tcagttccca ccatcactac cgatctttcc ggattcggac
    18121 agtggatgaa agataatttt gagaataaac acggtgcagc cgttgtacat cgttctgatt
    18181 acaatttctc tgaagtaacc gactcgatcg ctcaaatttt gaacgatttt ttgaatcaga
    18241 ataaaaagga aatggatgat gcaagaaaaa gagcatttat tacatccaaa aaagcattgt
    18301 ggagcgaatt cattcaatat tattacgagg cctattccag ggcattggaa aaaagaaata
    18361 aaagaataaa aaattttccg acaattaaat gaaaacgttt gaaattcaat ccacgatatt
    18421 tcttaatcct tactatagat tttaagaaaa taaatactaa ttttgtcgaa aatatgtttt
    18481 acagcatatt tttgaacata atacgacaaa aaataataaa ttaaaaattt caacaataga
    18541 tatgaaactt aaagcaaacg aagtaaacga accgacctgg agagatatcg ttgtcaaatc
    18601 aaacttaccg gaaagcctga acaagctgga agaactatcc caaaatatgt ggtgggcctg
    18661 gaacagtgat gcaaaaaaac tatttcgcga aatagaccgg ccaacatgga aagaagccga
    18721 gtcaaatccg gtacaactac tgaatctgtt gagctacgat aaacttgtag cactcggtaa
    18781 agatgaagaa ttcaccaaaa aagtagattc ggtttacaac gaatttaccg cctacatgaa
    18841 cgaaccgacc gacacaaaac ggccttctat agcctatttc agcatggaat acggattaag
    18901 tgaaattctg aagatttatt ccggaggttt gggagtactc gctggcgact acttgaaaga
    18961 agccagcgac agcaatatcg acatgactgc catcggtttt ctgtacagat acggctattt
    19021 caaacaatca ttgtcaatcg acgggcaaca aatttctatg tacgaaccgc aaaacttcaa
    19081 catcttaccc atcaagcaag tgttggatga gcaaaacctt ccttttgttg tcgaagtgcc
    19141 ttatccgggc cgcacggttt acgcacacct ctggaaagta tcggtgggaa gaatagccct
    19201 ctatctgctt gataccgaca ttgacgagaa tcatgattac gaccgctcaa tcactcacca
    19261 actgtatgga ggcgactggg aaaaccggat gaaacaggaa atcatgcttg gcataggcgg
    19321 catactggca ctcaacaaac tgggaatcaa aaaacaaatc tatcattgca acgaaggtca
    19381 tgcagccctc atcaatgttc agcgattggt tgatttgatt caaaacgaaa aactcacatt
    19441 cgcacaggcg ctcgaagttg ttcgtgccag ttcactttat acagttcata ctccagtacc
    19501 ggccggacac gatagttttg acgaaagtct cttcagtaaa tacatgagcg aatatcctgc
    19561 caaactgaat atatcctggg ccgatttcat tgacatggga cgtacaaatc ccggcaccaa
    19621 cgaaaaattc aacatgagcg tgtttgcatg tcacacatcg caggaagtaa acggcgtaag
    19681 ctggcttcac ggcgaagtat caaaacagat gtttgcacct atctggaaag ggtatttccc
    19741 ggaagaatct catgtaagct atgtgaccaa cggcgttcac ttaccttcgt ggacatcatc
    19801 ggaatggaaa gcgctttatg aaaagcattt caccaaagca ttctacgaag accagtcgaa
    19861 cgtaaaaata tgggaaaaga tttaccaggt tccggatcag gaaatctgga acatccgtac
    19921 atcgctgaaa aacaaactgg tagaatacat ccggatgcag ttccgcgata cgtggttgaa
    19981 aaatcagggc gatccgtcca gaatcgtatc cattctcgaa gccattaatc ccaattcact
    20041 gatcatcggc ttcggacgcc gctttgccac atacaagcga gcacatttga tattcagcga
    20101 cctggaaaga cttgaaaaaa tcgtgaacga tccgattcac ccggttcagt tcctctatac
    20161 cggaaaagct cacccggctg acggtgccgg acagggattg atcaaaagaa ttgtagaaat
    20221 ttcgcgcatg ccgcagtttc tcggaaaaat cattttcctc gaaaattacg acatgagact
    20281 cgctaaacgg ctgatttctg gtgtggatat ctggctcaac acacctaccc gaccgcagga
    20341 agcttcgggt acatcgggcg aaaaagcatc catgaacggc gtgctgaact tctcggtgct
    20401 tgatggatgg tggtacgaag gttacagaaa gaatgccggc tgggcactta ctgataaaag
    20461 aacgtatgaa aatcaggaat atcaggataa actggacgcc attaccattt ataatacact
    20521 cgaagatgaa atcattccat tgtattttgc aaaaaacaca aaaggatatt cgcccgagtg
    20581 gattcagttt atcaaaaaca atattgctca aataacacca aattatacca caaaaaggat
    20641 gatggacgat tattttgacc gtttttatat gaaacaggcc aaaagaacag ccatgcttag
    20701 cgccaatcat ttccagactg ccaaagaaat tgccgactgg aaagaaaaaa tggccgaagg
    20761 atggaacaac atcgaggttg ttgcactgga tcttcctgaa aaactggtac aaaatccgat
    20821 ggcaggtgaa gattatagca ttaacatcac cctggatgtg aaacaaaaca acatgaaagg
    20881 cattggtgtt gaactggtag caatccagac agaccagaac aacaacagca aactgagcag
    20941 tgttgacgaa ctgacactgg ttaaaaccga cggtacaaag ctcacattca acatcagcta
    21001 tcaactggac aatgccggag catacaaata cggcttccgc atgttcccga aaaatgattt
    21061 gctaccacat cgacaggatt tctgctacgt gcgatggatt taatcaattg aataacagag
    21121 cattaaaaga ggctgaatca tcaaacgatt cagcctctta ttgtaagaaa aatatttcgt
    21181 taaactaacg tattcccctt tcgttgaggg ctttctgata tcgggcagca ttgacggtat
    21241 gttgagacca ggtagctgca aaattatgct cgccgttcag tgtttctttg gcacacatga
    21301 aaatgtaatc attcctgtcg tagttcaata cggcttcaag acccgaaata gtaggtattc
    21361 tgatgggtcc gggaggtaat ccggtattcc tgtatgtatt gtatggcgat tctatacgtg
    21421 tgtgtacatt gagcaccctg cgaagcgaaa aatcaccggt ggcaaatatc acggtaggat
    21481 ctgcctgcaa cggcatattt ttacgcagcc tgttgatgta caatcccgct actacgggct
    21541 tttcagcttt gctgtttgtt tctgcatcga cgattgaagc cagcgtactg acttcagcct
    21601 gtgtcatagg aattgcctct gctttacgct ttcgttcttc agtccagaac ttgtcatatt
    21661 ccttgtacaa tctttcgaac atgcgttttg cactcatatt ccaaaacact tcatacgtgt
    21721 tggggatgaa tgcagatacc gatgtgtagg gattcaggtt gtacccggcc aaaaaagcag
    21781 tatcattgag taattccagg atttcggccg aatcgggcat caactggctt gccaggcgtc
    21841 ctgcaagctg ttctttggtg cgtatattat taaatgtgag gtttacggga gtctgagtgc
    21901 cgttcctgag atttctgaca agcgtcaggt tgttcatccc gttctcaatt ttatacctgc
    21961 ccgattttac attcttataa cgcaggtatt tggcagcctg tctgaaagtg gtcacatttt
    22021 tcacatcggc ttttgcctgt agctgttgga gtacctcttc gaaaccggtg ttttctctga
    22081 taagcagaaa ttcattttta tctgttctga gtttcacgtt gggcaataaa aacaaatatg
    22141 ctacatagga aaagccgatt atggcaacca aagcaattaa aatgagtatt tttgcgactt
    22201 ttgattttaa catgtatctt tttctgatta aaaatttctg aattccggtt gattgtaata
    22261 aacatacaaa tttataaaat agttttttaa tttttagcac caaaaggttt tgttaatcgg
    22321 aaaaccttca ctatatttgc actcgcaaat ttcaaatcag aaacacgcct ggaagtgtgg
    22381 gtgagtggct gaaaccacca gtttgctaaa ctgacgtacg ggtaaccgta ccgggggttc
    22441 gaatcccccc gcttccgcaa aaaaaaagag accagttcat tcatgaatcg atctcttttt
    22501 tttatttatc ttttgaaagg tacgtactgc ggtgctttgg gttcgtattc ctcatcgtcc
    22561 caaagcggac tggtaatcat caggtcggcg ctgtagcggt tacaggcaat gggtacgttg
    22621 tgaattcggc attggcgcag aagcatctga atgtcggctt cgtgcggatt ggcgctgagg
    22681 tcatcaatta aaaacacaca gaaatcaatt tgtttattaa ccaccaaagc tgcaatttca
    22741 gcgtcgccac ccatcggacc ggagttcatt ctcttcactt ccacttcaat gccttcgtct
    22801 tcaaaagctt gctggataag tcctccggtt gttccggtgc agattaattt gtgctgtgaa
    22861 agcatatccg aattaaacac tgcccattca accatatcag ctttacgtgc atcgtgtgcc
    22921 acaagggcta ttttaagttg ttttttcata attttttctt tcattttttt gttttttaca
    22981 aagttatgcc ttttcagcaa acattgttta ttaaattcat tcttgaaatc gtttaatcgt
    23041 tacgttaaat tagaaatgca acaatcattc caaagcaaac aaatattaaa tacctgccaa
    23101 aaaggcagaa aaccgaattt atcaggaaaa gataaattcg gtttcaattc ttctcaaacg
    23161 atagagaatc gtttgtcaca cagttttaaa cccatttaag aagcttagaa aagggttttt
    23221 attcttccag taaaatttcc ataatagcta tagcagcttt tgtcaccgaa gttcctggac
    23281 caaaaatggc tgcaacacct gctttgtaca gataatcata atcctgcgcc ggaattacac
    23341 cgccagcaat gacaataata tcttcacgtc cgagtttttt cagttcgtcc attacctgcg
    23401 gaacaagcgt tttgtgacct gcagcaagcg aagaaacacc caaaatgtgc acatcgtttt
    23461 ccacagcctg tttggcagct tcggccggag tctggaatag cggccccatg tccacatcaa
    23521 acccgcaatc ggcataaccc gttgctacta cttttgcgcc gcggtcgtga ccgtcctgac
    23581 ccattttggc aatcatgata cgtggacgac ggccttcttt tttagcgaat tgttcggtga
    23641 gccctgatgc acgttcgaaa tcgctgtcat ttttagtttc tgatgaatac acgcctgaaa
    23701 ttgttctaat ggttgcttta taacgtccca cgattttttc gcaggcatct gaaatttcgc
    23761 ccagtgatgc acgcagacga gctgcttcca cagccaactc cagtagattt ccttcgcctg
    23821 tacgcacgca ttcggtgatt gcatcgagtg ctttctgaac tgctgcgtta tcccggttgg
    23881 tacgaagttc tttcagacgg gctatttgct gttcgcgaac ggccgtgttg tccacttcca
    23941 gaatatcaat gggatcttct ttttccaacc ggtattcatt cacaccgata attttctgag
    24001 cgccactgtc gatgcgtgcc tgagtacggg ctgctgcttc ttcaatacgc attttgggaa
    24061 ttccggtttc gatggctgct gccataccgc ccagtttttc cacttcttca atcagctccc
    24121 aagctttttc agccagttcg ttagtcagtg attccacata atacgaacct gcccacggat
    24181 ctacctcgcg ggtgatgtct gtttcctgtt gaatgtagat ttgtgtattg cgggcaatac
    24241 gtgccgaaaa atcggttgga agcgcgatgg cttcgtccag cgcattggtg tgaagtgatt
    24301 gtgtatgtcc gagtgcggct gccattgcct cgatggtcgt acgtcccaca ttgttgaacg
    24361 ggtcttgctc ggtgagcgac catccggagg tttgtgagtg tgtgcgcaaa gcgagcgatt
    24421 tcggattttt cggattgaac tgtttcacaa ttttcgccca aagcaaacga gccgcacgca
    24481 ttttggcaat ttccataaag tggttcatac cgattgccca aaagaacgac aaccgcggtg
    24541 caaaagcatc aatatccatt ccggcattca ctccggcgcg aaggtattcc agtccgtcgg
    24601 ccagggtgta agccaactcg atatcagccg tagctccggc ttcctgcatg tggtagcctg
    24661 aaatggagat tgagttgaat ttcggcatgt ttttagacgt atattcgaaa atatcggcta
    24721 tgattttcat ggagaatttc ggtggataaa tgtaggtatt ccgcaccata aattctttca
    24781 gaatatcgtt ctggatagtt ccggccattt cttccagttt ggcaccctgt tccagtccgg
    24841 cattgatgta aaacgccaga attggcagta ctgcaccgtt catagtcata gatacagaca
    24901 tctgattgag cggaattccg tcaaagagta ctttcatatc ttctacggag cagattgaaa
    24961 cacccgcttt acctacgtcg cccaccacac gttcgtgatc ggcatcgtaa ccgcggtgtg
    25021 tagccaggtc gaatgctacg gaaagccctt tctggccggc agccagattt cgacgataga
    25081 aagcgttact ttcttcagcg gtggaaaatc cggcatactg acgaattgtc catggacgca
    25141 tcacatacat ggtactgtaa ggaccacgca aaaaaggagc aataccggcg gcataattca
    25201 aatgttccat acctgcaagg tcttctttcg tgtattgagg tttcaccgcg atttgctcgg
    25261 ctgtcaacca acttccttcg gcttcgggag ctaccgtact tgccacgaag ttttgaatgt
    25321 caatttcttt aaaatttggt ttcattattt tgatagactt ttagaacaaa gacttttaga
    25381 ttttagaccc ttgttcatta tgttttatta atctttttta tcgctttttc ttttaactgt
    25441 atgtttgaat tttaaaactg caaaattaag ttctactgtt gtttctgtat ctgttgtttt
    25501 catatcttca cccaaaagat ccgacataat ttcaccgctt ctttgcatca atgatttttg
    25561 tttatcttca tctttgattt cagccaattc agccactgtt cgtgaaagtt cccgaatttg
    25621 ggtaaaagtt tcgataatgg caatggtggt ttgcgttgct ttttcacttt ttaaaatagt
    25681 ggcaagcata tacaatcctt tttcggtaaa agctttgggt agaactcggg tttttgataa
    25741 gtttgtggtc gaaaatttcg acatcaaatc cgcaagttct ttattgtcaa gctcaataat
    25801 atatcctgtt ggaaatttat cctggttgtt ttttacggct tcatttattc gttttgtttc
    25861 aacgccttac aattccgcta catcactatc aataatcact ttctgattac gtattgtaat
    25921 tattttatcg gcaacctggc tgaatttcac taacattttg agtatatcta tttatatatc
    25981 aataagataa aataataata gcaagatgat tgtattccta tttatgggat agatagcatg
    26041 tttacattat cggcaacaaa ataaacaccg ccacatccca cactgcatgt gaaatgacca
    26101 gtggcaccag gtttttgtta tatctgtaca tcaatcccca gaaaataccg cagaccaatg
    26161 cagccatcac gagcatgaaa ttgaaactcc agatatgcac cagagcgtat atcagcgtgg
    26221 caaatacaaa acctttccat tcaccgtatt tattgataaa attttgttgt acatatccac
    26281 gccaaaaaat ctcttcagca ggacccatga taaaaatcaa accaagagca atccacaatt
    26341 tactctgtcc ctctttcagc gcataaatat tgtccacctg ctctcttgca aagccgaaca
    26401 acaatcctga aaaatactca cccaggtaaa aaacaatgta aagtgccgta gcagctaata
    26461 atccaaatcc gatggctttc catgtccatt gaaattgttt cagaatttcc tttcgaagaa
    26521 tgatagcaac aaccatcaat accgatgcag aaatggcaat cccaatccaa aaattgaaca
    26581 aatgctttgt ccagatggaa aacatcagaa accacaatac ggcagctacc agaataggcc
    26641 cccagctgcg aaagatggga gttttgaaga acgtctgaac gggattatcc gttttttgaa
    26701 aattctgttt taccatatca aataataatc ttttttaagt tattcttaat actcctaaag
    26761 aggggcctgg ctgccgcata cagggttaag cgtttaaaaa tcctcctgca aagtttccaa
    26821 ttaccagaag caacatataa accatcacca gctgggctgt accctcatcg agtttctgga
    26881 tattttccgg tttttcctta tcggctgtac gcatttgttt cataagtttc agagccatcg
    26941 ggagtgagat gaaaatgagc aacgttgccc aatgctgaat tccaaaaata acacataaaa
    27001 ctattaataa atatgcaccg attgtaaatg caaagtaata aattattgaa ccctttattc
    27061 caagaagcat ggctgctgtt ttgatttttg ccacgccgtc gtgcttgata tcccttgtgt
    27121 tattggcatg gagtacgttg acaataagga atccgggtgc agcagcaacc agtagcattt
    27181 tccagctcaa tgcattggtc atcaccagat acg
//
LOCUS       DAORKP010000009        26861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_50_length_26861_cov_9.000597, whole genome shotgun sequence.
ACCESSION   DAORKP010000009 DAORKP010000000
VERSION     DAORKP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26861
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_26861_cov_9.000597"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1290)
                     /locus_tag="PLN75_01185"
     CDS             complement(76..1290)
                     /locus_tag="PLN75_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRG02759.1"
                     /translation="MSANQDSNSGQRYDLRGVSASKEDVHNAIKNIDKGLFPKAFCKI
                     IPDFLGNDPEYCNIMHADGAGTKSSLAYVYWRETGDLSVWKGIAQDALIMNVDDLICV
                     GATDNILLSSTIGRNKNRIPGEVISSIINGTSELVAELREMGVNIYETGGETADVGDL
                     VRTIIVDSTVTCRMKREDVIDNANIQAGDVIVGLSSSGQATYEKEYNGGMGSNGLTSA
                     RHDVFANYLAEKYPESYNPEIPQDLAYSGGKKLTDAIEGLQIDAGKLVLSPTRTYAPV
                     VKKMLETLRPHIHGMVHCSGGAQTKILHFIDNLKVVKNNLFPIPPLFKLIHAQSGTDW
                     KEMYKVFNMGHRLEVYLPREYAQQVIDISRSFNIDAQIVGYCEASDKKELVIESEFGR
                     YIYAPQSPKGEV"
     gene            complement(1654..4326)
                     /locus_tag="PLN75_01190"
     CDS             complement(1654..4326)
                     /locus_tag="PLN75_01190"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRG02760.1"
                     /translation="MQVLFKLNHRTNWGQSVTLLIYNESNSYDSFDMTCENENKWKVS
                     LTLPDDVNEINYRYAIRKANNDFRYEHDGIRKVSIKANLKSLVFEDYWRTPNGDSPFD
                     TTAFTACFFKRKKAAKTKSKEETNFKLQLRCPQLEPDRHFAITGNQAVLGNWDEDKKI
                     RLNETDFPLWNIDLNSKDIKFPLEYKYLIVDTKTDKVIAWGGGPNRNVKSIDPSAFTI
                     VTDENFIRTIPKWKTAGVAIPVFSLRSEESFGTGDFMDLKKMIDWAKATGQHIIQTLP
                     INDTILNHSNYDSYPYNAVSVYALHPIYLRLERVGMPRRKRDLAYFEKKKKELNENTF
                     SDYQNVLNEKWKYFRLIFEQEGEKLLQSDDFSQFYEKNRKWLVPYAVFSWLRDKYGTP
                     NSVKWPEYQAYNADEMEKLASPDSAYYKEISLFYFLQYHLHIQLIEVHQYAHASGVAL
                     KSDVPIGVSPRSVDAWVEPQLFNLNMQAGAPPDDFSVTGQNWGFPTYNWEKMAKDGYR
                     WWKERFRKFMEYFDAYRIDHILGFFRIWEIPENAVWAMTGSFSPSLPFSIQELQSRGL
                     WWDENRYLKPYLREHVIRAILGKYADDVMREYLQTDGWQNFRFKPEYDSQKKIEAHFA
                     ALGYNFTTKEVEIRDGLYALHCQVLLVRDKINPNKFHPRIAMHSSQTFKDLDEERRRC
                     FDRIYVEYFYHRHNEFWKEQAMKKLPAIISSTNMLACGEDLGMVPDSVPDVMNELEII
                     SLEIQRMPKKPNTEFAMPADAPYLSVCTSSTHDMNPIRAWWEEDPALTQRFYNRALGI
                     NGPAPETCLPMIAEKIIEQHLRSTAMLVILPWQDWMAMDKKLAHKNPHAERINIPADP
                     RNFWCYRMHLTLEQLLAEKKFNAKVKKMIKESGR"
     gene            complement(4472..4542)
                     /locus_tag="PLN75_01195"
     tRNA            complement(4472..4542)
                     /locus_tag="PLN75_01195"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4508..4510),aa:Cys,seq:gca)
     gene            complement(4724..5188)
                     /locus_tag="PLN75_01200"
     CDS             complement(4724..5188)
                     /locus_tag="PLN75_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02761.1"
                     /translation="MRNLRIYSQLLLLCLTLITTQAQNRRGKFDISAFHDKKWEFIIT
                     EISLSPAEANAIKPLFLDYEKKNWELHNNTRELFRQSRSGNLTENQYRDLNDKMINNE
                     IKRSQYLREYHLKLRKLLKPNVLFRYYRAEKNFERQLLNKRPAGPPPTPEMQ"
     gene            complement(5196..5519)
                     /locus_tag="PLN75_01205"
     CDS             complement(5196..5519)
                     /locus_tag="PLN75_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02762.1"
                     /translation="MEENEYIEIQKAGNRNPFKLPENYFEEFAARMDEMAADTPKEVK
                     RFIIRPWMYGAAASLAGVLLMGQVYLSDNKKQKLASENYDTYVLSQVNESSIIDYYLA
                     SETEK"
     gene            complement(5638..6111)
                     /locus_tag="PLN75_01210"
     CDS             complement(5638..6111)
                     /locus_tag="PLN75_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRG02763.1"
                     /translation="MVRTYQERLYWHIRKMVLVHDDANDVLQNTFMKAWKGLDNFRGD
                     SQISTWLYRIATNETLSFLSQKKARMMNSFEDVEELMLQNLQADTYFEGDEVQLKLQK
                     AIITLPEKQRLVFNMRFYDEMPYEEMSKILDTSVGALKASYHHAARKVEDFLIKE"
     gene            complement(6234..7253)
                     /locus_tag="PLN75_01215"
     CDS             complement(6234..7253)
                     /locus_tag="PLN75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRG02764.1"
                     /translation="MSGKINKYHNYFFLIGVLVLAFMVYKLGIQTIYENLRNTGWWFI
                     PIFGIWIIVYFFNAWSLNIIITDGTPESKKVKIRQLMKLTISAYAINYMTPLSIGGEP
                     YKALKLKDDLGTHKATSSVLLYVMMHYVSHFFFWLISIPVFIFIMPEVSGVIQIVLWA
                     VFLSCVMLILWAYSVFTKGVIKNALSIATRFPFVGRKIRLYRDKNQHHLNEMDLLISD
                     LYTNRKKDFFASLFVELISRYISCFEVYFMAIPLAFDLSFIQSYLIVSIATLIANIFF
                     FAPMQMGTREGGFVLATTTLALPAGIGVYIGLCTRIRELFWIMIGVLLTKIKLPAKKF
                     QQTLP"
     gene            complement(7294..8202)
                     /locus_tag="PLN75_01220"
     CDS             complement(7294..8202)
                     /locus_tag="PLN75_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRG02765.1"
                     /translation="MTQKDKKSEYLSSLKSIETENFLDRIFYRPVGYKIAKALQNTGI
                     TPNVITIISIFVGVSAGVFWWFPYNITFAVMGILALIVANILDCVDGQLARLTGIKSQ
                     VGRILDGLAGDLWFLMVYIAFVHRLDIQFSTPYDPWIYIAVILLSAISHWNQASVTDY
                     YKTLHLLFISNDKGKEFENSKLIAGRYKKMKRGVNKVITWGYILYTKNQERLTPELQK
                     MMGRLQSKYGNDIPEDVRKAFRAKSSKIMPMIDLNTFNGRSFILFISVIFNIIWVYFV
                     WEIVVLNINRMIIRNRHEKVCKSIDN"
     gene            complement(8252..9595)
                     /locus_tag="PLN75_01225"
     CDS             complement(8252..9595)
                     /locus_tag="PLN75_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG02766.1"
                     /translation="MKKVITYGTFDLFHQGHINLLNRAKSLGDYLIVGVTTDNYDRGR
                     GKLNVHRSLMERINDVREAGFADEIIIEEYEGQKIDDILKYGVDIFAIGSDWLGKFDY
                     LKEYCQVVYLERTKGVSSTQLRIEAQSVTELGIIGSGRIAAKFVPESKYVSGIEVKGV
                     FNPHIDSARIFCEKHELSFYTDDFEEFISKINAVYIAAPHHTHSDYIRRCLTNGKHVL
                     CEKPLALTARETEEMYKLAESKGLVLMEAIKTAYCPAFSHLITVVKSGVIGSIKDIDV
                     SFTMLCSDTLHNPEAEEGGGSITRLASYVLLPIFKILGYNYNDIQFYSHMVNEIDIFT
                     RGLIIYDKAIASFKVGLGVKTEGDLIISGTKGYAYVPAPWWKTEYFELRYEDQNQTRK
                     YFYKFDEEGLRYEINDFLIEMNKPNQKQFKLQASDSIAIASIIEKFRKIENVHLI"
     gene            complement(10121..10849)
                     /locus_tag="PLN75_01230"
     CDS             complement(10121..10849)
                     /locus_tag="PLN75_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRG02767.1"
                     /translation="MEKNILITGGTKGIGFAVAKSLVTAGYNVILTYGSDFHQAEKAR
                     MELHQAGKKSVMVVKADITEKKSIDILDDFLAEHNIRLFSVVFNAGITCRTPFESLTI
                     DEWEQTFFANLHFPVFLLQRIVHRIDKGGNVLFTGSLMGEYAHSVSLAYGVSKSAVHA
                     LVKNLVKFMQPFSIRVNAVAPGFVDTDWQKNKPAEIRKNIENKIALGRFCSPDELAQV
                     YKMIIENNYFNGEIIPVSGGYSYR"
     gene            11147..12349
                     /locus_tag="PLN75_01235"
     CDS             11147..12349
                     /locus_tag="PLN75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG02768.1"
                     /translation="MNAITSTARNKKIFGFSPNVFYTGIVSFLTDTSVKMVYSVMPMF
                     LLSIGASKTSLSLIEGIAESTASLLKAFSGYWSDKIGKNKPFMLIGYGLSALVMPLYA
                     AVVSPLHVLLLRFTERLGKGIRTAPRDSLVAGSVEKNETGKSFGLHKAMDNSGAILGP
                     LLAFLILSMKPDDFRFIFLLAGIPGILGVIVLIFGIKEAKKNKESLLQKFRFQDYPKR
                     FYLFLGIIFIFTLGNSTDALLIVKANEVGIKVTFIPLVYLISSVVSVFLAIPLGTLSD
                     KIGREKLLIFGFMVYAVVYFGFGITGDIYTIIALFGLYGVYSASTDGIQKALVSDFTD
                     RNKKGTAMGLYNALLGVTLLPASLIAGVLYDKVNSSIPFYFGATTAVISAILMVIFVV
                     TKPKVSVK"
     gene            complement(12370..12879)
                     /locus_tag="PLN75_01240"
     CDS             complement(12370..12879)
                     /locus_tag="PLN75_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRG02769.1"
                     /translation="MKRLNKQFYTLGAVEMAQQLLGKTLVRVFDDGTIKHYQITVTEA
                     YCGSEDRACHASKGRTTRTEVMFHGGGKIYVYLIYGMYWMLNVVAGTENNAQAVLICG
                     IDEIKGSGRVGRQLKIDKTFYGEDLEISERIWIEDSDENPDFNAKPRVGVDYAGEWKN
                     KLWRFEVTK"
     gene            12991..14520
                     /locus_tag="PLN75_01245"
     CDS             12991..14520
                     /locus_tag="PLN75_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRG02770.1"
                     /translation="MFDNQHRSFVAINDEKEICFIPKMANRHGLITGATGTGKTISLQ
                     VLAETFSNMGVPVFAADMKGDLSGVAKVGGNKESVTKRVTQFKLAEKGFEYKSCPVQF
                     WDVFGEQGHPVRTTVSELGPLLLERLLMLNETQGAVLSLIFKIADENDLLLIDLKDLQ
                     KMLQYVGDNRAQFTTKYGNISSASVGSIQRNLARLEAEGGEVFFGEPELNISDLIKTD
                     NRGKGIINILAADKLMNSPRIYTTFLLWLLSDLFENLPEVGDMEKPKLVFFFDEAHML
                     FNDMPKILLEKVEQIVRLIRSKGVGVYFITQNPADIPENILGQLGNRIQHALRAYTPN
                     DQKAVRVAANTFRANPKFDTETAITELGTGEALVSFLDEKGAPNPVERAFVLPPEGQI
                     GPVTPDERMHVIQSSLLYGIYEKEVDRISAFEIISERMNADDLQKQQLEQQKQLEIQR
                     RDQEKADREAERQRRAAAKDDVFGGLLKDVGRTVGRQMTSQIGRSITRTILGAFFGKK
                     R"
     gene            14616..15869
                     /locus_tag="PLN75_01250"
     CDS             14616..15869
                     /locus_tag="PLN75_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRG02771.1"
                     /translation="MRKTSNLIFILILSIFVTTQKVNAQQFATGADMSWLTEMEAAGR
                     KFYSADGIQTECLTLLKSLGTNAVRLRVWVNPANGWCNAADVLAKARRASDLGMRIMI
                     DFHYSDSWADPGKQFKPAAWTSLDLNGLKTAVANHTKDVLNLLKNNNITPEWVQVGNE
                     TGNGMLWDTGKASVSMANYAALTTAGYDAVKEIFPSAKVIVHVHNGYDNGLFRWLFDG
                     LKNNGGKWDVIGMSLYPGWYTVKNDWAAANTACLANMNDMVSRYNKEVMVVECGMSWD
                     MAATCKNFLTDLIAKTKSVSNGKGLGVFYWEPQSYGQWNGYTLGAFDNSGKPTVALDA
                     FRNSTGMDQVTDNMLKILYSKATKSIIFGETLKSVSIINAEGKLILIRENTNKLSTQQ
                     LSAGIYVVKAVSHSGEKLIRKIVIN"
     gene            15960..16475
                     /locus_tag="PLN75_01255"
     CDS             15960..16475
                     /locus_tag="PLN75_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRG02772.1"
                     /translation="MALLIVGLTLPFLSCSSIPKGVKAVDNFEKDKYLGKWYEIARFD
                     FRFERNMNNTTANYSLNENGTIKVVNRGFNYKKQEWTEARAKAKFVGKEDVAMLKVSF
                     FGPFYSGYNVIALDKDYKYALVSGQSLDYLWILSRTTTIPDDVKQQYLKLASDLGYKT
                     ENLIWVEHNKN"
     gene            complement(16644..17252)
                     /locus_tag="PLN75_01260"
     CDS             complement(16644..17252)
                     /locus_tag="PLN75_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRG02773.1"
                     /translation="MKNLSLILNGILLIAVIVLFALVLGKKNSGTVPVVTKKGDSAVV
                     EKLPLAYINIDSLLLNYTFAKNANEVLISKQESSRATLNSRARQLQTEMDEFNRKLEN
                     QAFLSRERAEEAQRNIVQKQQDLQKMEANLSQQLMEQQQKMSEQLRDTINAFLKVYNK
                     DGKYQMIISNTANDNILYAGKGYDITSDVIEKLNARHTKGKK"
     gene            complement(17258..17494)
                     /locus_tag="PLN75_01265"
     CDS             complement(17258..17494)
                     /locus_tag="PLN75_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02774.1"
                     /translation="MDLLAAVLFTIVIVFLAVFLLGFRIFLSKNGKFPNIHIGGSKAM
                     KDRGVSCATSQDAEAQKNNLRKIDVSKIINEIDK"
     gene            complement(17692..18267)
                     /locus_tag="PLN75_01270"
     CDS             complement(17692..18267)
                     /locus_tag="PLN75_01270"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRG02775.1"
                     /translation="MNFSLPQLPYAHNALEPVISEETIKFHYGKHHQTYVNNLNGLIP
                     GTEFENADLDTIVKKSSGPVFNNAAQIWNHNFYFLSLTPEKGSKPSEELAKAIDEKFG
                     SLDNFKAEFNKSAVAVFGSGWAWLVKTADGGLDIVKESNAGNPLTSGLTPLLTFDVWE
                     HAYYIDYQNRRADYVAALWELVDWNVVSSRY"
     gene            18448..18897
                     /locus_tag="PLN75_01275"
     CDS             18448..18897
                     /locus_tag="PLN75_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRG02776.1"
                     /translation="MKKVLGLMLLAAVVLAGSTSCKSKQKVVNISGSDIEAAETKPVT
                     QPVVVEEIESKETTRGEKFNLADGETNSAAFNKKFHVVVGSFGVQNNAKNLQRTLNSE
                     GNSAIVVVNEQGMFRVLIASYDEYAQARNRISQISNRFPDAWVLIQK"
     gene            18973..19455
                     /gene="folK"
                     /locus_tag="PLN75_01280"
     CDS             18973..19455
                     /gene="folK"
                     /locus_tag="PLN75_01280"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRG02777.1"
                     /translation="MAFIYLGLGTNLGDKVQNLNTAIQKIGIEIGDVWNVSAFYPSKA
                     WGYSSENNFLNAVILVSSQLTPPEVLTKTKQIEKKMGRTEKPDNKYHDRIIDIDILMY
                     DNLTISLPNLQIPHPWMHQRDFVLNPLEEIAPEMVHPVLNETIASLNSKLKKEKEKTQ
                     "
     gene            complement(19457..19723)
                     /locus_tag="PLN75_01285"
     CDS             complement(19457..19723)
                     /locus_tag="PLN75_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRG02778.1"
                     /translation="MEEDKLVSVKYYETVIEAEIDQNVLKTNGIECIYNDDTVVGVFP
                     IFDDKERGIELMVFEKDVEKARQILDEYHAAVDTVADSDEDNQE"
     gene            19817..20797
                     /locus_tag="PLN75_01290"
     CDS             19817..20797
                     /locus_tag="PLN75_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRG02779.1"
                     /translation="MSFYSKYKSKIRKIRRKIVLLVLFPPLLLFIFLFRILPISWVRL
                     FAAFSGKYFYRFATKSREWALANLERIYGDTLAENERIGIAKASFTEILTGFFDYMAY
                     SHVKDASRYFRLIEVDGEEHLRNAYHRGKGVICLIPHMSSWEFAAITPPMLGYATSAA
                     SQSMKMSLLEKLIVKFRSSRGMKNITRQGSYQALTEALNKGECLILMTDQDTRVKGIF
                     MDFLGYPAYTPLGASRLLADTQAALVPMAMTRKEDGNYHFLIFPEIPTIRTDNPEADL
                     QTNTGNQNKFYSEIIRKYPAQWVWMHRRWKTTPESLAATLKARKESTQNK"
     gene            20838..21443
                     /locus_tag="PLN75_01295"
     CDS             20838..21443
                     /locus_tag="PLN75_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRG02780.1"
                     /translation="MDENQLIYSKPVIEFATVAAESCLFLERAEEHTKTDFITTATRL
                     LPLLYLKTSLLDLPEDESEGNIERFVTEDDYLFVKNQIERLLGADDSFLEVFHPDMPY
                     SDTPIAAFVSENLADIYQEIKDFAANFQIGESEIMTAALAACLSAFAEHWGQKLLNAL
                     RALHSVKYSENFGEEETDDNLDPKEYRKLNRDSFLKFQSED"
     gene            21457..22113
                     /locus_tag="PLN75_01300"
     CDS             21457..22113
                     /locus_tag="PLN75_01300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRG02781.1"
                     /translation="MFRPTITKEEVNQLPIVEFSGKIILVDTPEKEEKAINELYKATI
                     VGIDTETRPSFKRGVLHKVSLMQFADNQTCYLFRLNTIGFSEKLIAFLSDKEIIKIGL
                     SLRDDFNGLARQKKFKPEGFVDIQNIAKEYGILELSLQKIYAILFGLKISKSQRLTNW
                     ENPTLTEQQQRYAATDAWATLQIYLRLTTEKKLTIKEQLELKAGLNTEQKPLSQQQNK
                     "
     gene            22110..23288
                     /locus_tag="PLN75_01305"
     CDS             22110..23288
                     /locus_tag="PLN75_01305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRG02782.1"
                     /translation="MIIIKLKPKKEESLLRFHPWVFSGAIQTINGKPSEGELVEVVDN
                     QNNFLAIGHYQIGSIAVRIVTFMKEEINNDFWKNKIKQAYILRCELGLVNPPQNNTFR
                     LIHGEGDNLPGLIVDVYDDTAVLQAHSVGMHMMRETLAKSMLEIVPAVKNVFYKSETT
                     LPFHAPIEKEDGYLIGKANKSFNAIENGLEFSVDWLKGQKTGFFVDQRENRILLEKYS
                     RGKNVLNMFCYTGGFSVYALRGGASLVHSVDSSAKAIELTDKNIRRNFPEATNHRSFA
                     EDAFKFFGDKRADYDLIILDPPAFAKHRDAVRNALKGYKRLNLKALQQIKPNSILFTF
                     SCSQVISRDQFRLAVFSAAAEAKRNVRILHQLSQPADHPINIYHPEGEYLKGLVLYVE
                     "
     gene            23508..24101
                     /locus_tag="PLN75_01310"
     CDS             23508..24101
                     /locus_tag="PLN75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRG02783.1"
                     /translation="MNNLSQLTDDDLVKLYEKGNNSAFEVILNRYKSRVFTYIFLIVR
                     NKELTEDIFQEAFIKAISTIQQGRYVESGRFLGWINRIAHNLIIDHFRREKNENTFST
                     DGLEYDFVNNSKYSEKSFEDVINNEQVLQDIVHLVNYLPSSQQEVVRMRFFEDLSFRE
                     IAERTDVSINTALGRMRYALMNMRKIASENNVQLEMK"
     gene            24180..24362
                     /locus_tag="PLN75_01315"
     CDS             24180..24362
                     /locus_tag="PLN75_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02784.1"
                     /translation="MPMQKDFTPVISTQSSHFQEIPEIIETEPSESTLNKIMQYAASY
                     RVEKVSDDEFIDYFLN"
     gene            24561..25481
                     /locus_tag="PLN75_01320"
     CDS             24561..25481
                     /locus_tag="PLN75_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02785.1"
                     /translation="MKKSKLLLLGFLLLSFTFSSAQVLQFTPYDELPELNKSYKPTYS
                     DDMPEWGKMLYSYPINFDVIDRAFVKWEAANKDKKTPLMRYYKIWRKAITPYVGADGE
                     IETPDVKLINQNLIRFQTQSLQPRRAPAAANSSNWTFWGPKETYWLNESGSATVPKTA
                     PWQANVYSFDVTDKNPNLLYAGTETGFINKTTDKGKNWELKGTNYPFGGGAGAIAIHP
                     VNLDTVLVGAGTAIHITMNGGETWKRTISSVGGINRMKYDYNNPDKAIACSSNGIYYS
                     NNSGINWYKSANTDTWVMNTNPVKAILYML"
     gene            25520..>26861
                     /locus_tag="PLN75_01325"
     CDS             25520..>26861
                     /locus_tag="PLN75_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02786.1"
                     /translation="MNGGKNFSPVTNFPDTLINHSGGLLAVTPHDPDIVLVIVLAKNM
                     NNRPFIYQGTNKQGNWTWIRKFVGSDASFTSNALTSGQGYFDLVLEISPVNKNIVYAG
                     TTTLFKSTNGGASFSAVGGYQGSFGIHPDIQDMKLLANGDTWVATDGGMNLSTNNFTT
                     SAAHFALNKMLVGSDLWGFHQGWNEDIIVGGRYHNGNTSITDFYGEKALRMGGGESPT
                     GWVIQGKSRHVVFDDLGSGWILPKTAEGKPEGRFTFSKHPNMEGYGALRSNVVAHPYY
                     SGQLYVGSENALWVSKDFGSTFDLLYDFGSKVRYFDISTQNPDVIYVDVAGSGFFRSE
                     DGGKTFIKKVSIAGSTGGNLRFVISPYSSDVVYATLTHGVWDETRSEIYKSTNGGQTW
                     EKWSTFGTSILIKTIAIQPTTDGEDLIYAFIDSYGYNSGTVKYRKGNGAWIDFGT"
BASE COUNT         7981 a         5560 c         5457 g         7863 t
ORIGIN      
        1 ctgcccccta accccctgaa gggggaatat tagtagggtt attcaagaat tttataaaag
       61 ttcaattttt acgtattaaa cttccccttt aggggattga ggggcatata tataccttcc
      121 aaactcactc tcaatcacca gctctttctt gtccgaagcc tcgcaataac ccactatctg
      181 cgcatcgata ttgaaggatc tggaaatgtc aattacttgt tgagcatact cccggggcag
      241 gtaaacttcc aaccggtgtc ccatattgaa cactttatac atttctttcc agtcggtgcc
      301 tgattgtgcg tggattagtt tgaaaagagg aggaatgggg aaaagattgt tttttaccac
      361 tttcagattg tcgataaaat ggaggatttt ggtctgtgca ccaccactgc aatgtaccat
      421 tccgtggata tgcggacgaa gcgtttcgag catttttttt accaccggtg cgtaagtgcg
      481 tgtaggtgaa agcaccagct ttccggcatc aatttgcagt ccttctattg catcagtcag
      541 tttcttcccg cccgaatacg ccaaatcttg cggaatctcc ggattgtaac tttcgggata
      601 tttttcagcc aggtaattgg caaacacatc gtggcgcgca gaagtaagtc cgttgctgcc
      661 cattccgccg ttgtattctt tttcgtaagt agcttgccca ctcgacgaaa gtcccacaat
      721 cacatcgccc gcctgaatat tggcattgtc aatcacatct tcacgtttca tgcggcaggt
      781 tacggtgctg tccacaataa tcgtacgcac caaatcacca acatcggccg tttcaccacc
      841 cgtttcatag atatttacgc ccatttcacg cagttcggct accaactcgc ttgttccgtt
      901 gattatagaa gaaatcactt cacccggaat gcggttttta ttccgaccga tggttgacga
      961 aagcaggata ttatccgtag cacccacaca aatcaaatca tccacgttca ttatcaacgc
     1021 atcctgcgca atgcctttcc aaaccgacaa atcgcccgtt tcgcgccagt acacgtatgc
     1081 cagcgaggat tttgttccgg cgccatcggc atgcataatg ttgcagtatt cggggtcatt
     1141 tcccagaaaa tcaggtataa ttttgcaaaa agctttcgga aaaagacctt tgtcgatatt
     1201 tttaatggcg ttatgcacat cctcttttga agctgaaaca ccacgcaggt cgtagcgttg
     1261 cccgctattt gagtcttgat ttgctgacat aatttgaatg atgtagctga atgattttga
     1321 attgcaaagg tagtaaaaac aaagagtgtt tgaaaggaaa aaaaacggaa gttggaagta
     1381 cgaagcttgt atttacgtct aaaaaaaatt aagaaactta tttgataaag gttaggtgct
     1441 acgcaccttt tttctctctt ttcatttatt tgctacaaat agtttggtgc tacgcacctg
     1501 aaaggtacgg agtgccgtac catttgtagg cagtcgcacc ccgagttatc ggttgataaa
     1561 ccgtgaaaag ttggatattc tttcatcgga tgttaaatgt actactcgga gtgcgactaa
     1621 aataagtctc cttttggggg atttaggggg cttttatctt ccactctcct taatcatctt
     1681 tttcactttc gcattgaatt ttttctctgc aaggagttgt tccaatgtta aatgcattcg
     1741 gtagcaccag aagttgcgcg gatctgcagg gatgttgatt cgttcggcat gcggattttt
     1801 gtgagccagt tttttatcca tcgccatcca gtcctgccac gggagtatta ccagcatggc
     1861 tgtggaacgt aaatgctgtt cgatgatttt ttcggcaatc attggcaagc atgtttcggg
     1921 agcgggtccg ttaattccaa gtgcacggtt gtagaagcgt tgagtgagtg ccggatcttc
     1981 ttcccaccag gctcgaatag gattcatatc gtgtgtggat gaggtgcaaa ccgataaata
     2041 cggagcatct gccggcatgg cgaattctgt attcggtttt ttgggcattc gctgaatttc
     2101 caggctgata atctccagtt cgttcatcac gtcgggtacc gagtcgggta ccatgcctaa
     2161 gtcttcacca catgccagca tattggtcga cgaaatgata gccggcagct ttttcatggc
     2221 ctgctccttc cagaattcat tgtggcgatg atagaaatat tccacgtaaa tgcggtcaaa
     2281 acatcttctg cgctcttcat cgaggtcttt gaatgtctga gaactgtgca tggctatgcg
     2341 gggatgaaat ttattcggat tgattttgtc tctcacaagg agcacttggc agtgcagggc
     2401 ataaaggcca tcgcgaattt cgacttcttt ggttgtaaag ttataaccta atgctgcaaa
     2461 atgagcttca atttttttct gactatcgta ttcgggcttg aacctgaaat tctgccaccc
     2521 atctgtctga aggtactcac gcatcacatc atcggcatat tttccaagaa tggcacgtat
     2581 tacatgttcc cgcaggtaag gtttcaggta gcggttttca tcccaccaca ggccccgcga
     2641 ctgtaattcc tgaatactga aaggtaacga agggctgaaa cttccggtca ttgcccatac
     2701 agcattttcg ggaatttccc aaatgcggaa aaagcccaga atgtggtcaa tgcggtaagc
     2761 atcaaagtat tccataaatt tacggaatcg ctccttccac catcggtaac cgtcctttgc
     2821 cattttctcc cagttatacg tagggaagcc ccagttctga ccggtaactg agaaatcatc
     2881 gggtggtgca cctgcctgca tattgagatt aaatagttga ggctctaccc aggcatccac
     2941 actacgcgga cttacgccta tgggaacatc gcttttgagt gccactccgg atgcatgagc
     3001 atactgatgg acctcaataa gctgaatatg aaggtgatat tgaagaaaat aaaacaaaga
     3061 gatttctttg taataagcag agtcggggct ggccagtttt tccatttcat cagcattata
     3121 cgcttgatat tcaggccatt ttaccgaatt cggtgtgccg tatttatctc tcaaccatga
     3181 aaacacggca taaggaacca accatttcct gttcttttcg tagaattgag agaaatcatc
     3241 agactgaagc aatttttcac cttcctgctc aaaaatcaaa cggaaatatt tccatttctc
     3301 attgagtaca ttctgataat ccgagaaagt attttcgttt agttctttct tctttttttc
     3361 aaaataagcc agatctctct tcctgcgtgg cattccgaca cgttccagcc gtagataaat
     3421 gggatgcagc gcatataccg atacggcatt gtaaggatac gaatcgtagt tggagtggtt
     3481 gagtatagta tcatttatag gtaaagtctg gatgatgtgc tgtccggtgg cttttgccca
     3541 gtcgatcatt ttttttaaat ccatgaaatc gccggtaccg aaactttctt cgctccggag
     3601 cgaaaatacg ggtatggcaa ctcctgcagt tttccatttt ggaatggtac ggataaagtt
     3661 ttcatccgtt acgatggtaa atgctgacgg gtcaatggat ttaacattac ggttgggccc
     3721 gccaccccag gcaatcacct tatcggtttt agtgtcaact atcaggtatt tatattcaag
     3781 tggaaattta atatctttgg agttgagatc aatgttccag agagggaaat cagtttcgtt
     3841 aagtctgatt tttttatctt catcccagtt gcccagcacc gcctgatttc ccgttattgc
     3901 aaaatgacgg tcaggttcga gctgaggaca gcgaagttga agtttgaaat tggtttcttc
     3961 ttttgattta gtctttgcgg ctttttttct tttgaaaaaa caggctgtaa atgcagttgt
     4021 atcaaaggga ctatcgccgt ttggtgtgcg ccagtaatct tcaaatacaa ggcttttcag
     4081 gttagctttt atgctcactt tccggatacc gtcgtgctcg taccggaagt cattatttgc
     4141 ttttctgatg gcataccgat agttaatttc atttacatca tcaggcagtg tcagagatac
     4201 tttccattta ttttcatttt cacaggtcat atcaaaactg tcataggaat ttgattcgtt
     4261 gtaaatgagc aatgtaaccg attgacccca atttgtcctg tgatttaatt tgaagagtac
     4321 ttgcatgata aattattttt tacgattaca aaaataaaag aaacaagctt tgttttaatg
     4381 taaatgaata tcaagtagtc gaaaagtcat ttaaaacgtt tgcataaaaa aaagagtcaa
     4441 ctacatgatg atatagttaa ctcttcggat gaggtaccta gcagattcga actgctgtag
     4501 acggttttgc agaccggtgc ctagccactc ggccaaggta ccatttttta cggactgcaa
     4561 agatagtaca atttttatgt tttatcaaaa tttaagtctg aaaaataaga attctccatg
     4621 tttacagttg gatttggact aaccttaatt agataaaaaa atggcgcaga ataattttcc
     4681 tgcaccattt tccaaaccaa tctattatta actatcgaaa aaactattgc atttcaggtg
     4741 ttgggggtgg ccccgcaggt cgtttattca ggagctgacg ttcgaaattt ttttcggccc
     4801 ggtaatatct gaacaacacg ttgggtttaa gcaatttacg aagcttaagg tgatattcac
     4861 ggaggtattg tgagcgtttt atttcattgt ttatcatttt atcattcaga tcgcggtact
     4921 gattttcggt caggtttccg ctgcgcgact gacgaaagag ttctcttgtg ttattgtgca
     4981 attcccagtt ctttttctcg taatccagaa ataaaggttt gatagcattg gcttcagccg
     5041 gtgagaggct gatttcagta atgataaatt cccacttttt atcgtgaaac gcactgatat
     5101 caaattttcc gcgcctgttt tgtgcctgcg ttgttatcag tgtgaggcac agcaacaata
     5161 gttgggagta tattcgtaaa tttctcattt tgtatctatt tttctgtttc tgaagctaaa
     5221 taatagtcaa tgatggagga ttcgttcact tgtgagagta cgtatgtgtc gtaattttca
     5281 gaggctagtt tttgcttttt gttgtcggac aaataaacct gacccatcag taaaacccct
     5341 gcgagtgaag ctgcggcacc atacatccac ggacggatga tgaaacgctt cacctctttc
     5401 ggagtatcag ctgccatctc atccattcga gctgcaaatt cctcgaaata gttttcaggc
     5461 agtttgaacg gatttctgtt gcctgctttc tgaatttcta tgtattcgtt ttcttccatt
     5521 ttcttattta gacttggtga aatttcaaaa gtttaaaact cattttgtta aaaaaatcct
     5581 gtttttattc aaaatcaatc gcgacaatca gcgtggagca tttttgtatt gcattgttta
     5641 ttcttttatc agaaaatcct ccactttccg ggcagcgtgg tgatacgatg ctttgagtgc
     5701 cccgacggat gtatcgagaa ttttgctcat ttcctcgtag ggcatttcgt cgtaaaagcg
     5761 catattgaac accagtcgtt gtttttcagg caaagtgatt attgcttttt gaagtttcag
     5821 ttgcacttca tctccttcaa aataagtatc tgcctgcaaa ttttgcaaca tcaattcctc
     5881 cacatcttca aacgaattca tcattctcgc ttttttttgt gaaagaaatg acagtgtttc
     5941 attcgtagca atacgataaa gccaggttga aatctgcgag tcgccccgaa agttatccag
     6001 ccctttccag gctttcatga atgtgttttg cagtacatca ttggcgtcat cgtgtaccaa
     6061 aaccattttt cggatatgcc agtagagccg ctcctgataa gtgcgtacca tttccgaaaa
     6121 agactttgct ttctcatcgg cagtttgacc tgaagtattt gctaagttca aataaaacgt
     6181 tgttttttta tttatacttt tatgactttc agaaaaatga aaagtttaaa aacctaaggg
     6241 agagtttgct gaaatttctt tgctggtaat ttaattttgg tcaacagaac accaatcatg
     6301 atccagaata gttctctgat acgtgtgcat aaacctatgt acacgccaat gcccgctggc
     6361 aatgccagtg tggtggtggc aagtacgaat ccgccttcgc gcgtgcccat ttgcatgggt
     6421 gcaaaaaaga aaatattagc aatgagtgta gctatcgaaa caatcagata tgactgtatg
     6481 aatgatagat caaaagcaag cgggatggcc atgaagtaca cctcgaagca tgagatgtat
     6541 ctggagatta actctacgaa aagtgaggcg aaaaagtctt tcttccggtt ggtgtacagg
     6601 tctgaaatca aaagatccat ttcgttcagg tgatgctgat ttttgtctct gtacaaacga
     6661 attttccgtc ctacgaaggg aaatcgggtg gcaatggaca gtgcattttt aataactcct
     6721 ttggtaaaaa cagaatatgc ccataaaata agcatcacgc acgacaggaa aactgcccaa
     6781 agtacaatct gaataactcc ggacacttcg ggcatgatga aaataaatac aggaatggaa
     6841 atgagccaga agaagaaatg cgatacatag tgcatcatta cataaagcaa taccgatgaa
     6901 gtggctttgt gggttcccaa atcgtctttc agtttgagcg ccttgtaagg ttcgccgcca
     6961 atactcagcg gtgtcatgta gttgatggca taggcgctga tggtaagctt catcagctgc
     7021 cttattttca cctttttgga ttcgggcgtt ccatcggtaa tgataatatt cagcgaccac
     7081 gcattgaaaa aataaacaat tatccatatg ccgaaaatag gaataaacca ccagccggta
     7141 ttgcgaagat tttcgtaaat ggtctgaatt ccaagcttat acaccataaa agccagaacc
     7201 agtaccccga taaggaagaa ataattgtgg tatttattta tttttccgga catgcattac
     7261 gacgtttaaa tactcggttt caaaacatca ggattaatta tcaatacttt tgcatacttt
     7321 ctcatgacgg tttctgataa tcatacggtt gatattcaaa acaacaattt cccaaacaaa
     7381 atacacccag atgatattga aaataactga gataaacagg ataaatgagc gtccgttgaa
     7441 agtattcagg tcaatcattg gcatgatttt gctgcttttg gcacgaaatg ctttacgaac
     7501 atcctccgga atgtcatttc cgtattttga ttgaagccgg cccatcattt tttgcaattc
     7561 cggagtaagg cgttcctgat ttttggtgta aagaatgtac ccccaggtaa ttaccttatt
     7621 tacacctctt ttcatttttt tataccgtcc ggctatgagt tttgaatttt caaactcttt
     7681 tcccttgtca ttgctgataa aaagtaagtg aagtgttttg tagtaatcgg ttacagatgc
     7741 ctgattccag tgggatatgg cagatagcag gattacggct atgtaaatcc agggatcgta
     7801 aggagtggaa aactgaatat ccagccgatg aacaaatgca atataaacca tcagaaacca
     7861 caaatcgcct gccagtccat ccagaattcg tcctacctgc gatttaattc cggtaagccg
     7921 tgccaactgt ccgtccacac agtcgaggat attagcaaca attaatgcca gaatccccat
     7981 aacagcaaaa gtgatattgt atgggaacca ccagaatact cccgcactta caccgacaaa
     8041 aatcgaaata atggttatta cgtttggagt tataccggta ttttgcagcg ctttggctat
     8101 tttgtaacca acggggcgat agaaaatcct gtcgaggaaa ttttcagttt cgattgattt
     8161 cagcgaactt agatattcgc tttttttatc tttttgtgtc atttatttgt atgagcttat
     8221 tctaaatcag cttatcggat tttggatttt actaaatcag gtggacgttt tcgatttttc
     8281 tgaatttctc aattattgag gctatggcta tcgagtctga tgcctgtaac ttaaattgtt
     8341 tctggtttgg cttattcatt tcaattaaaa aatcgttgat ctcataacgt aaaccttctt
     8401 cgtcgaactt gtaaaaatac tttcgcgttt gattttgatc ttcgtatctg agttcgaaat
     8461 actcggtttt ccaccacgga gccggtacat aggcataccc tttcgttcca ctgattatca
     8521 gatcgccttc cgttttcact cccaaaccca ctttgaagga ggcaatagct ttatcgtaga
     8581 ttatcagtcc acgggtaaat atgtcaatct catttaccat atgagaataa aactgtatat
     8641 cgttatagtt atatcctaaa attttgaaaa tcggtagcag tacatacgat gcgagccttg
     8701 taatactgcc tcctccctcc tctgcttcag gattatgtaa tgtatcgctg catagcatcg
     8761 taaaggaaac atcaatatcc ttaatactgc ctatcactcc gcttttcacc actgtgatga
     8821 ggtgtgaaaa agccggacaa taggctgttt tgattgcttc catcaggaca agacccttac
     8881 tttcggccag tttatacatt tcttcggttt cgcgggcggt gagagcaagg ggtttttcgc
     8941 acaacacatg tttaccgttc gtaaggcaac gccgtatata atcggaatga gtatgatgtg
     9001 gtgctgcgat gtaaacggca tttattttcg aaataaattc ttcgaaatcg tcggtataaa
     9061 aactcaattc atgtttttca caaaaaattc tggccgaatc gatatgaggg ttgaaaacgc
     9121 cttttacttc aatgccactc acatattttg attcaggaac aaacttcgcg gctatgcgtc
     9181 cgcttccgat aatgcccaat tcggttaccg actgtgcttc aattctcagc tgtgtgctcg
     9241 atactccttt ggttcgttcc agatacacca cctgacagta ttctttgagg tagtcgaact
     9301 tgcctaacca gtccgaaccg atggcaaata tatcaacccc gtatttgagt atatcatcaa
     9361 ttttttgtcc ttcatattct tcaatgataa tttcatcggc aaaaccggct tctcttacat
     9421 cattaatccg ttccatcagc gaacggtgta cgttgagctt gcctctgccc cggtcgtaat
     9481 tgtcggtggt tacgcctaca atcaaataat ctcccaatga ctttgcccga tttaacaggt
     9541 tgatgtggcc ctgatgaaac agatcgaatg ttccgtatgt aatcactttt ttcatgtgtt
     9601 tattttttat tttttacact atggaaacat gtaacacgcc atttatcatc tcattgtgtt
     9661 tgagaggttt atctaaagga tgtttcatct gatttattta tatttatttg tcgggagaga
     9721 attgatcatt gaaatatcca ttttagtttg ttctgttatt tcaaatggac gtttcgtttt
     9781 tttcaaaatt tcacgttcgt atcctgttct cacttttcat tgccgcatct gccatcctga
     9841 aagcatatgt atcaaaaata tttgaatttt tctggtatta cacttgcaag ttccggaaaa
     9901 attcagattt atgaaaaaca aatgacaaag ttagtaattt tttaatttat ttaatccatt
     9961 ccaagttcca aaaattcatt cacaagtaca tagagataaa atcaaaggca ttttatcgtg
    10021 taaatcaata ctgtaaaact tattttcttt tttttcagaa atacatatat caatcattat
    10081 caaatcttta ccactaaaaa aaatctgagt tcaaaaaaaa tcatctgtaa gaatatcctc
    10141 cgctaaccgg tattatttcc ccgttgaaat agttgttctc tataatcatt ttatatacct
    10201 gagccagttc gtcgggacta caaaaacgtc ctaacgctat cttgttttca atatttttcc
    10261 ttatttcggc gggtttgttt ttctgccagt cggtatccac gaagcctggt gctacagcgt
    10321 tgaccctgat gctgaatggt tgcataaact tcaccagatt tttcaccaat gcatgtactg
    10381 ccgatttgga tactccgtag gcaagtgata cagaatgagc atactcaccc atcagcgaac
    10441 ctgtaaacag cacgtttcca cccttatcta tccggtgaac aatgcgttgt agcaggaata
    10501 cgggaaaatg aaggttggca aaaaaagttt gttcccattc gtcgatagtc agactctcga
    10561 agggtgtacg gcaagtgata cctgcattga aaacaactga aaacaaacgg atattgtgtt
    10621 cggcgaggaa gtcatcgagt atgtcaatcg attttttttc agtgatatcg gctttcacca
    10681 ccatcaccga ttttttgcct gcctggtgta actccatgcg tgctttttct gcttgatgaa
    10741 aatccgaacc ataagtcagt atgacattat atcctgctgt aaccaggctt ttggcaaccg
    10801 caaaaccaat gcctttagtt ccaccggtta tcagtatgtt tttttccatt taagaattct
    10861 aatcatatat tcacactaaa atgcgacttg tgtttgcaac atgcatttcg acattttctt
    10921 aagtgtggca aagataatta ttttttcatg aatgtaacaa ttttgtaata ttaaaaaagc
    10981 gaaaaatgta actaccgaac atcagctcgg ttattgaaaa tttttccagt aaaattttga
    11041 taaaacaaag atttatttgc attgaaaaaa gagcgagaac tagtactttt gcattgttta
    11101 tatttcttga tgttttcatt tcaagcatca ttctgttgaa attagcatga atgcaatcac
    11161 atcaactgcc cggaataaaa agattttcgg cttttcgccc aatgtttttt acacaggtat
    11221 tgttagtttt ctcacagata cttctgtaaa aatggtctat tcggtcatgc ctatgtttct
    11281 gttatctatc ggagcatcca aaacgagtct ttcgctcatc gaaggcattg ctgaaagcac
    11341 cgcatcgctg cttaaggcat tttcgggata ctggagcgat aaaattggga aaaacaaacc
    11401 ctttatgctg attggttacg gactttcggc gcttgttatg ccactttacg ctgcggtggt
    11461 gagtccgctg catgtgcttt tgcttcgatt tacagagcgc ttgggtaaag gcatccggac
    11521 tgctccacgc gatagtctgg tagccggttc tgtggaaaaa aacgaaaccg gaaaaagttt
    11581 tggtttgcac aaagcaatgg acaacagcgg agccatactc ggtccgttgt tggcattttt
    11641 gatactttcc atgaagccgg atgattttcg atttatattt ctgctggcag gaattcccgg
    11701 aattttagga gttattgtat tgatttttgg gataaaggaa gcaaagaaga ataaggaaag
    11761 tctgctccaa aagttcagat ttcaggatta cccaaaacgg ttttatctgt ttctgggaat
    11821 aatttttatt tttacacttg gaaattctac cgatgcgctt ttgattgtaa aagccaatga
    11881 agtgggcata aaagtgactt tcattccgtt ggtttacctc atttcaagtg tagtctccgt
    11941 ttttctggcc attcctctgg gtacactgtc cgataaaatc gggcgggaaa aattgctgat
    12001 attcggattt atggtttatg cggtggttta tttcggattt ggaattacag gtgatattta
    12061 taccatcatt gctttgtttg gcttgtacgg agtttattct gcatcaaccg acggaattca
    12121 gaaagcgctg gtttccgatt ttaccgacag gaataaaaaa ggaaccgcaa tgggtcttta
    12181 caacgctttg cttggagtta cgctgctgcc cgccagtttg attgccggag tactttacga
    12241 taaagtcaat tcaagcattc ctttctattt cggagcaacg acagcagtaa tttcagccat
    12301 tttgatggtg atttttgtag taacaaaacc aaaagttagt gttaagtagc tgctaacttc
    12361 agttagctgc tacttagtca cttcaaaccg ccataattta tttttccact ctccggcata
    12421 atccacgcct accctgggtt tagcattaaa atccggattt tcatcgctat cttcaatcca
    12481 aatcctttca gaaatctcta aatcctctcc gtaaaatgtt ttatcaattt tgagctggcg
    12541 ccccaccctg cccgagcctt ttatttcatc gattccacaa atcagtactg cctgggcatt
    12601 attctcggta ccggcaacca cattcagcat ccagtacatg ccataaatca aataaacgta
    12661 tattttccca ccgccgtgaa acatcacttc ggtacgcgtt gtgcgtccct tactggcatg
    12721 acaagccctg tcttcactac cacagtaggc ttcagttact gtaatttggt aatgctttat
    12781 tgttccgtca tcaaacaccc gtaccagcgt ttttcccaat aattgttgtg ccatctcaac
    12841 agcacccagc gtgtaaaatt gtttattcaa tcgtttcata aaggcaaaat taatctttta
    12901 attccaaaca ttgcacaatt gaagaaaaac agtaaatttg caattacaac ttgaaattat
    12961 tcactttttt ctaaaaaaaa atacagcgat atgtttgaca atcaacaccg ttcgtttgtg
    13021 gcaattaacg acgaaaaaga aatctgtttt atcccgaaaa tggctaaccg ccacggattg
    13081 attaccggag ccaccggtac aggtaaaacg attagtttac aagtacttgc agaaactttc
    13141 agcaatatgg gggtacccgt tttcgccgca gatatgaaag gcgacctctc aggagtggcc
    13201 aaagtgggcg gaaacaaaga aagtgtgaca aaacgtgtga cacagttcaa gctggctgaa
    13261 aaaggtttcg aatacaaatc ctgcccggta caattctggg atgtatttgg agaacaggga
    13321 catcccgtca gaaccacggt ttctgaactc ggaccgctgc tcctggagcg tttgctaatg
    13381 ctcaacgaaa cacagggagc cgtactttca ttgattttca aaatagctga cgaaaatgat
    13441 ttattgctta tcgacctgaa agatttgcaa aaaatgctgc aatatgtggg cgacaaccgg
    13501 gcacaattca ccactaaata cggaaatatt tcatcggcaa gtgttggctc catccaacga
    13561 aatctggcac gcctggaagc cgaaggaggc gaggtattct ttggtgaacc ggagctgaac
    13621 atctccgact taatcaaaac cgacaaccgc ggcaagggaa ttatcaacat cctggcagcc
    13681 gacaaattga tgaattcacc ccggatttac accacattcc tgctttggct tctctctgat
    13741 ttatttgaaa atctgcccga agtgggcgat atggaaaaac caaaactggt gtttttcttt
    13801 gacgaagcac acatgctttt caacgacatg ccgaaaatcc tgctcgagaa agttgaacaa
    13861 atcgttcgcc tgatacgctc caaaggtgta ggagtatatt tcatcaccca aaatccggct
    13921 gacatacccg aaaatattct gggacaactc ggaaatcgca tacagcacgc actgcgtgcc
    13981 tacacgccca acgatcagaa agccgtaaga gtagctgcta atactttccg cgcaaacccg
    14041 aaatttgata ccgaaacagc catcaccgaa cttggcaccg gagaagcgct ggtatctttt
    14101 ctggacgaaa aaggtgcacc caatccggta gaacgcgcat tcgtacttcc tcccgaaggt
    14161 cagattggtc ctgtcactcc cgatgaacgt atgcacgtaa ttcagtcttc gctgctttat
    14221 ggcatttacg aaaaagaagt ggatcgtatt tcagccttcg aaataatttc ggaacggatg
    14281 aatgccgacg acctgcagaa acaacagctg gaacagcaaa aacaacttga aattcagcgt
    14341 cgtgatcagg aaaaagccga tcgggaagct gaacgacaaa gacgcgcagc cgcaaaagac
    14401 gatgtattcg gcggattact caaagacgtg ggacgcactg taggccgtca gatgacttct
    14461 cagataggaa gaagcattac cagaactata ctgggggctt tctttggaaa gaaaagataa
    14521 tcaccggagt cgtttttttc ttaaataatt ttcattctcc aacgcaccgg tatttatcgg
    14581 tgtgttgttt tttggacaaa taaatttttt tttcaatgcg aaaaacaagt aatctgattt
    14641 ttatactgat tctgagcatt tttgtaacta ctcagaaagt gaatgcacag caatttgcta
    14701 ccggagccga tatgagctgg ctcacagaaa tggaagcagc cggcagaaaa ttttactctg
    14761 ccgacggcat tcagaccgag tgcttaacgc tgctgaaatc gctgggcacc aatgcagtaa
    14821 ggctcagggt atgggtaaat cctgccaatg gctggtgcaa tgctgccgat gtactcgcaa
    14881 aagcacgccg cgccagcgac ctgggaatgc gaattatgat agactttcat tacagcgact
    14941 cctgggccga tcccggcaaa caattcaaac cggctgcatg gacttcgctc gacctgaacg
    15001 gacttaaaac ggccgtagcc aaccacacaa aagacgtact gaacctgctt aaaaacaaca
    15061 atataacccc ggaatgggtg caggtaggca acgaaacggg taatggcatg ctttgggata
    15121 ccggaaaagc atccgtgagc atggccaact acgcagcact gactactgcg ggctacgatg
    15181 cagtgaaaga gattttcccc tcagccaaag tcattgttca tgttcataat ggctacgaca
    15241 acggtttatt ccggtggcta ttcgacgggc tgaaaaacaa cggaggcaaa tgggatgtga
    15301 taggtatgtc gctttatccc ggctggtata cggtaaaaaa cgactgggca gccgccaaca
    15361 cagcctgcct ggcaaacatg aacgacatgg taagccgcta taacaaggag gtgatggtag
    15421 tggaatgtgg catgagctgg gatatggcag ctacctgcaa aaactttctg accgacctga
    15481 tagccaaaac caaaagcgta tccaatggaa aagggctcgg agttttctac tgggaacctc
    15541 aatcatacgg acagtggaat ggctacaccc tgggtgcttt tgacaacagc ggcaaaccga
    15601 ccgtagctct ggatgctttc agaaacagca ccgggatgga tcaggtgact gacaacatgc
    15661 tgaaaattct ttacagcaaa gctacaaaaa gtataatatt tggtgaaacg ctgaaatcgg
    15721 taagtataat caacgctgaa ggaaaactca tcctgattcg tgaaaataca aataagctga
    15781 gcactcagca actttcagcc ggtatttacg ttgtaaaagc agtatctcat tcaggtgaaa
    15841 aactaatcag gaaaattgtt attaattagt tcttaccctt cttttttaaa aaaataagaa
    15901 aactaaatgc aacaacataa ataacataaa aaaagatgaa aactaaaaca aaaacactta
    15961 tggcactact aattgtgggt cttacattac cgtttctgtc ctgttcgagt attccgaaag
    16021 gagtgaaagc tgtagataat ttcgaaaaag ataagtacct gggtaagtgg tatgaaattg
    16081 cccggttcga ttttcgcttc gaacgaaaca tgaacaacac cactgcgaat tattcgctca
    16141 acgaaaatgg tacgattaaa gttgtaaacc gcggatttaa ctacaaaaaa caagagtgga
    16201 cagaagccag agctaaagca aaattcgtgg gcaaagaaga cgtagccatg ctgaaagtgt
    16261 cgtttttcgg ccctttctat tcgggctaca acgtgatagc gctggataaa gattataaat
    16321 atgcactcgt ttcggggcaa agtctggatt atttgtggat tttatcgcgc acaaccacca
    16381 tcccggacga tgtgaagcag caatacctga aactggcttc agaccttggt tacaagaccg
    16441 aaaatctgat ttgggtagaa cacaataaaa actgacattg aattttttat ccggtattaa
    16501 actctttttc caggtgaaaa attatcatca acctgttttt catttcttct gaacacctaa
    16561 taagttcaga aaagttcaga aaaaaaacgg agtcggcttt cacaagctga ctccgttcat
    16621 ttagctacaa ttttgaattg aacttatttt tttccttttg tatggcgggc atttaatttt
    16681 tcaattacat ctgaagtaat atcataacct tttccggcat acaggatatt atcattggct
    16741 gtattgctta taatcatctg atacttacca tctttgttat atactttaag aaatgcattg
    16801 atagtatcac gcagctgctc gctcattttc tgctgctgtt ccatcagttg ctgcgaaaga
    16861 tttgcttcca ttttttgcag atcctgctgt ttttgtacaa tattgcgctg tgcttcttca
    16921 gctctctcac gactcagaaa tgcctgattt tcaagtttgc ggttgaattc atccatttct
    16981 gtctgaagct ggcgtgctct ggaatttaag gttgctcttg agctttcctg cttgctgatt
    17041 agcacttcgt ttgcattttt ggcaaatgtg tagttcaaaa gcagcgaatc gatattgata
    17101 tatgccaaag gcaatttttc aacaacagcc gaatcgcctt tttttgtaac aaccggaaca
    17161 gtaccgctat tcttttttcc aagcacaagt gcaaacaaaa caattacagc tatcagtaaa
    17221 ataccattta aaataagtga gagattcttc attttgttta tttatctatt tcattaatta
    17281 ttttagaaac gtctattttt cgcagattat tcttctgagc ttctgcatcc tgcgatgttg
    17341 cgcaacttac tcctctgtct ttcatggctt tgctcccacc gatgtgaata tttggaaatt
    17401 taccgttttt tgacaggaat atcctgaaac ccagtaaaaa aaccgccaga aaaacaataa
    17461 cgatagtgaa taaaacagct gccagaaggt ccataattga taattgaatt tatcggtcgc
    17521 aaagataatt gttttgaaac atattaaatg aataggtatc caatttttag aaaaatgaat
    17581 gtttgaatcg gcgtttttaa cttttgttaa aacagacagg cactaaaaaa ccgaaagttg
    17641 taaattgctt tcggtttttt cggttatgca acatattatg actaatagaa attaatacct
    17701 ggacgaaacc acgttccagt ccaccagttc ccagagtgca gctacataat cggctctacg
    17761 attctggtaa tcaatataat aagcgtgttc ccacacatca aaggtaagca gtggagtgag
    17821 tccggaggtg agtggatttc cggcattgct ttcttttact atgtcgagtc cgccgtcggc
    17881 agttttcacc agccaggccc atcctgaacc gaatactgca actgcagatt tgttgaattc
    17941 ggctttaaaa ttatccagcg aaccgaattt ttcgtcaata gctttagcca gttcttccga
    18001 tggtttgctt cctttttcgg gagtgagcga tagaaaatag aagttgtgat tccaaatctg
    18061 agcggcattg ttgaaaaccg gaccacttga ttttttcacg attgtatcca gatcggcatt
    18121 ttcaaattca gtgcctggaa tgagtccgtt gaggttgttc acgtaagttt gatgatgttt
    18181 accatagtga aactttatag tttcttcact aattaccggc tccagcgcgt tgtgagcata
    18241 gggcagttgg ggcaatgaga aattcataat aattgtattt tttttagtta ataatgagtt
    18301 ttacaagtat cggttaaaca ctgagattat agtttttgtt tatctctaat atgtaaaatt
    18361 tgcaaaaatc agctttattt gttgggaata aacaaatttc ttttcatctt tgtagtctga
    18421 taaaatttta agttaaaaaa aaaagatatg aaaaaagttt taggtttaat gttgctggct
    18481 gcagttgttt tagcaggttc aacatcttgc aagtcaaagc aaaaagtagt taacatctca
    18541 ggatcggata ttgaagctgc cgaaacaaaa ccggtaaccc agcctgtggt agtagaagaa
    18601 atcgaaagta aagaaaccac aagaggcgaa aaattcaatc tggcagacgg cgaaaccaac
    18661 agcgctgctt ttaataaaaa attccatgtg gtagtaggaa gttttggagt gcaaaacaac
    18721 gccaaaaacc ttcaacgcac actgaacagc gaaggcaaca gtgccatcgt ggtagttaat
    18781 gagcaaggaa tgtttcgggt gctcattgca tcttacgatg aatatgctca ggcacgtaac
    18841 cggataagcc agattagcaa ccgttttccg gatgcatggg tgctgataca gaaataaatt
    18901 acacagaaca ctctaacaaa cggctttgta actaatacaa ggtcgtttga ttttttaaaa
    18961 tttcttaaac agatggcatt catttacctg ggattaggaa ccaatctggg agataaagta
    19021 cagaatctga atacggctat tcaaaaaata ggtattgaga taggtgacgt gtggaatgtt
    19081 tcggccttct acccatccaa agcctggggt tacagttccg aaaacaattt tctgaatgcg
    19141 gtcattctgg tgagcagcca gctgacaccg ccggaggttt taacaaaaac caaacaaatt
    19201 gaaaaaaaga tgggacgtac agaaaagccg gataacaaat accacgacag aatcattgac
    19261 attgacatcc tgatgtacga caacctgaca atcagtttgc ccaaccttca gattccacat
    19321 ccgtggatgc atcaaagaga ttttgtgctg aatccgctgg aggaaattgc acccgaaatg
    19381 gtacatccgg ttctgaacga aacaattgcc tcgctaaatt caaaactgaa aaaagaaaaa
    19441 gaaaaaacgc aatagcctat tcctgattgt cttcatccga atcagctact gtatcaaccg
    19501 cggcatgata ttcatcgaga atttgtctgg ctttttctac atctttttca aaaaccatga
    19561 gttcaatacc acgctccttg tcatcgaaaa tggggaaaac tcccaccaca gtatcgtcgt
    19621 tgtaaataca ttcaatacca ttcgtcttga gcacgttttg gtctatttcg gcttcaatca
    19681 ctgtttcata atatttcact gacaccagtt tatcttcttc catggctttg tttgtttatg
    19741 ttttgttagt ttttaaaaat tcagtcattg atataacatt aacagcttgt tttttgtttc
    19801 atttcaaatt cagtatatgt cattctattc taaatataaa tcaaagataa gaaaaatccg
    19861 gcgcaaaatc gttttgctcg ttcttttccc gccattactg ttatttatat ttcttttcag
    19921 aatacttcca attagttggg tcagattatt tgctgcattt tccggaaaat atttttaccg
    19981 gtttgccacc aaatcacgcg aatgggctct tgccaatctg gagcgtattt acggagatac
    20041 gcttgcagaa aatgagcgaa tcggtattgc aaaagcatca tttactgaaa tacttacggg
    20101 atttttcgat tacatggcat actcgcacgt gaaagatgcg tcccggtatt tccggctgat
    20161 tgaagtggac ggtgaggagc atttgcgtaa tgcataccac agaggtaagg gtgtgatttg
    20221 cctcattcct cacatgagtt cctgggaatt tgcagccatc acaccaccta tgctgggtta
    20281 tgcaacctct gcagccagcc agtccatgaa aatgagtctg ctcgaaaaac tgattgtgaa
    20341 gttcaggagc agccggggga tgaaaaatat tacccgccag ggctcatatc aggcattgac
    20401 cgaagcactc aacaagggag aatgcctgat actgatgacc gatcaggaca cccgcgtgaa
    20461 aggaatattt atggattttc tcggatatcc ggcatatacg cctctggggg catcgcggct
    20521 gctggctgat acgcaggcag cgctcgtacc catggcaatg acccgaaaag aggatggaaa
    20581 ctaccatttt ttaatcttcc ctgaaatccc gacaatccgg acagataatc cggaagccga
    20641 tttgcagaca aacaccggga atcagaataa attctactcc gaaataatcc gaaaatatcc
    20701 cgcgcaatgg gtgtggatgc accgaaggtg gaaaaccaca cccgaatcac tcgccgcaac
    20761 attgaaagca cgaaaggaat caacacaaaa taaataagat attttttgct aactttgcac
    20821 attattattt tgaaaaaatg gacgagaatc aactcatata ttcaaaacct gtgatagagt
    20881 ttgctacggt ggcagcagaa tcatgtctgt ttctggaacg tgccgaagaa cataccaaaa
    20941 ccgattttat caccacagcc accagactgc ttccactgct gtacctgaaa acttcattgc
    21001 tggaccttcc ggaagatgaa tcggaaggaa acatcgaacg atttgtaaca gaagatgatt
    21061 atctgttcgt aaaaaatcag atagagcgtc tgctgggagc cgacgacagt tttctggaag
    21121 tatttcatcc ggatatgccc tacagcgata cgcccattgc tgctttcgtt tccgaaaatc
    21181 tggccgatat ctatcaggaa atcaaggatt ttgctgcaaa tttccaaatc ggtgagtccg
    21241 aaatcatgac tgctgcgtta gcggcatgcc tgtcagcttt tgccgaacat tggggccaga
    21301 aactgctcaa cgccctcagg gctttacatt cggtgaaata cagtgaaaac ttcggtgagg
    21361 aggaaacaga cgacaacctc gatccaaaag agtacagaaa gctcaacagg gatagtttcc
    21421 ttaaattcca gagtgaagat taattgcccg taaaaaatgt tccgacccac cattacaaaa
    21481 gaagaagtca accagctgcc gattgtcgag ttttccggaa aaatcatact ggtagatacc
    21541 cccgaaaagg aggaaaaagc cattaatgaa ttgtacaaag caactatagt tggcattgac
    21601 acagaaacaa ggccatcgtt caaacggggt gtgcttcata aagtatcact catgcaattc
    21661 gctgacaatc agacatgtta ccttttccgc ctgaatacaa tcggcttttc cgaaaaacta
    21721 attgcttttt tatccgataa agaaatcatt aaaataggat tatcacttcg cgatgatttc
    21781 aacgggctgg ccaggcaaaa gaaattcaaa cccgagggat tcgtggacat acagaatatt
    21841 gccaaagaat acggaattct ggaactgagc cttcagaaaa tttacgccat ccttttcggc
    21901 ctgaaaatat caaagtcaca acgacttacc aactgggaaa atcccaccct gactgagcaa
    21961 caacaacgtt acgctgcgac tgacgcctgg gctaccctgc aaatctacct acggttaaca
    22021 accgagaaaa aactgactat aaaagaacag ttagaactta aagccggcct gaacaccgaa
    22081 cagaaaccac ttagccaaca gcaaaacaaa tgataatcat caagttaaaa ccaaaaaaag
    22141 aagaaagtct gctgcgtttt catccctggg ttttctcggg agccattcag accattaacg
    22201 gaaaaccatc cgaaggagaa ttggtagaag tagttgataa tcaaaataat tttctggcta
    22261 tcggacatta ccagattggc agtattgctg ttcgtatcgt aacatttatg aaagaggaaa
    22321 tcaacaacga tttctggaaa aataaaataa aacaagctta catcctgcgc tgtgagctgg
    22381 gtttggtaaa tccaccccaa aacaacacct ttcgcttgat tcatggtgaa ggcgataatc
    22441 tgcccggact gattgtagat gtttatgatg atacggccgt gcttcaagcg cattctgtag
    22501 gtatgcacat gatgcgcgaa acactggcaa aatccatgtt agaaatcgtt ccggcagtaa
    22561 aaaacgtttt ttataagtcg gaaactaccc ttccttttca tgcgccgatt gaaaaagagg
    22621 acggttatct gattggcaag gccaataagt ctttcaacgc gattgagaat gggctggaat
    22681 tttcggtaga ttggcttaaa ggacaaaaaa ccggtttttt cgtggatcag cgggagaaca
    22741 gaatcttgtt ggagaaatac tccaggggaa aaaacgtgct gaacatgttt tgctacaccg
    22801 gcggtttctc cgtgtatgca ttgcgtggcg gagccagtct ggttcattct gtggatagtt
    22861 ccgcaaaagc catcgaacta accgacaaga atatccgtag aaatttcccc gaagcaacca
    22921 atcaccgatc ttttgcagaa gatgcattta agtttttcgg tgataaaagg gctgattatg
    22981 acctgataat actggatcct ccggcgtttg ccaagcaccg cgatgctgtc cgcaatgctt
    23041 tgaaaggata taaacggctg aatctaaaag cattgcagca aatcaagccc aattccatcc
    23101 tgtttacttt ttcctgttcg caggtaattt ccagggacca gttccgtttg gcagttttca
    23161 gcgctgccgc tgaggctaag cgaaatgtcc gcattctgca ccaactcagc cagcccgccg
    23221 accacccgat taatatttac cacccggagg gagaatacct gaaagggctg gtgctgtatg
    23281 tagaataacg ggggagtttt ttttcaacac agtaaaatta ttgttgaaaa atgacctaat
    23341 ttaacttttt taattatttt taacgcaatt atcgccttct aaatttgaaa aacaaaaatg
    23401 aatgcataaa tttgcatccc gtttcctgtt aaaacaatat aaacatttaa taccattcat
    23461 acttgcctat tgcctaagat tacttgataa ccccaaaaat ttaagtaatg aacaatttaa
    23521 gtcaactaac cgacgacgat ttggtgaagt tgtatgaaaa aggcaataat tcagcttttg
    23581 aagtaatatt aaacaggtat aaatcccgtg tttttaccta tatttttctg atagtccgaa
    23641 acaaggaact caccgaagat atttttcagg aagcttttat caaggcaatt tctacaattc
    23701 aacaaggtcg ttatgttgaa tccggccgat tcctgggctg gataaaccgt attgcacata
    23761 atctgatcat cgatcatttc cgcagagaaa aaaatgagaa cacattttcg acagacggtt
    23821 tggaatacga tttcgtaaac aattccaaat actccgagaa aagtttcgag gatgtgatca
    23881 acaacgagca agtgcttcag gatatagttc atctggtcaa ttacctccct tcctcacaac
    23941 aggaagtagt acggatgcgt ttttttgaag atctgagttt cagggaaata gccgaacgta
    24001 cagatgtaag catcaataca gcactcggac gtatgcggta tgcactgatg aacatgcgaa
    24061 aaattgcgag tgaaaacaat gttcagcttg aaatgaaata atttttttgc gtaaaaaaaa
    24121 acagggtaaa atatactata atttgaataa aagcgtttga taattataaa acatatcaaa
    24181 tgcctatgca aaaagatttt acccctgtta tttcaactca atcgtcacat tttcaggaaa
    24241 ttccggaaat catagagact gaaccaagcg aatcaaccct gaacaaaatc atgcagtatg
    24301 ctgcctcata ccgagttgaa aaagtctctg acgacgaatt tatcgattat ttcttaaatt
    24361 aactgaaaaa gagagtaaaa aaaattactc tctttttttt aggaaatgca ttgataaata
    24421 ttctattgta caatagtctg ataatcaaac acaaaaaaat agctgagaaa caaataagaa
    24481 ttgttaaaaa aacggatttt tcatcaaatt tcttgactat tatataactt tgcacactga
    24541 aaacctcaaa aatcaaagaa atgaaaaaat ccaaactcct cctattgggc tttttgctcc
    24601 tgtcatttac attcagcagc gcccaggtac tgcaatttac accttacgac gagcttcctg
    24661 aattaaacaa atcctacaaa ccgacttatt ccgacgatat gcccgaatgg ggaaaaatgc
    24721 tgtattccta tcccattaat ttcgatgtga tagatcgggc attcgtgaag tgggaagcag
    24781 ccaacaagga taaaaaaact ccactgatgc gctattataa aatctggcgc aaagcaatca
    24841 caccctatgt gggagctgat ggcgaaatag aaacaccgga tgtaaaactc attaaccaaa
    24901 atctgatcag gtttcagact caatcactac aaccccgacg cgcacccgct gcagcaaatt
    24961 catcaaactg gaccttctgg ggtccgaaag aaacgtactg gctgaacgaa agcggaagtg
    25021 ccacagttcc caaaacagct ccatggcaag caaatgtata ttctttcgat gtaacagata
    25081 aaaatcccaa tttactttac gcaggaactg aaacaggctt tatcaacaaa actaccgata
    25141 aaggtaaaaa ctgggaacta aaaggcacga actatccttt cggaggtggt gccggggcaa
    25201 tagccataca tcctgtaaat cttgacacag tacttgtagg tgccggtacc gctattcaca
    25261 taacaatgaa tggtggcgaa acctggaaaa gaacaataag ttcagtagga ggaattaacc
    25321 gcatgaaata tgattataac aatcctgaca aagcaattgc ttgttcgtca aatggtattt
    25381 actattccaa caatagtgga atcaactggt ataaaagcgc gaataccgac acctgggtaa
    25441 tgaatacaaa cccggtcaaa gcgatactgt atatgcttta acaaaaaata cttctgaaaa
    25501 attcactttc attcaatctg tgaacggtgg caagaatttc agtcctgtta ccaattttcc
    25561 ggatacatta atcaaccatt ccggtgggtt gctggcagta actccgcatg atcctgatat
    25621 cgtgctggta atcgtactgg ctaaaaacat gaacaaccgt ccatttattt atcagggaac
    25681 caacaaacag ggtaactgga catggattcg caaatttgta ggatccgacg cttctttcac
    25741 aagcaatgca cttaccagcg gacaaggata ttttgatctc gttcttgaaa tttcacctgt
    25801 aaataaaaac attgtatatg caggaactac taccctgttc aaatctacca acggaggagc
    25861 atctttttca gccgtaggtg gatatcaggg ttctttcggg attcaccccg atattcagga
    25921 catgaaattg ctggccaacg gagatacctg ggtagcaact gacggaggta tgaacctgag
    25981 caccaataat ttcaccacat ctgctgctca ctttgccctg aataaaatgc tggtgggctc
    26041 tgatctttgg ggatttcatc agggatggaa tgaagatatc atagtaggag gaagatatca
    26101 caacggaaac acatccataa cagattttta cggagaaaaa gcactgcgta tgggtggtgg
    26161 cgaatctcct acaggctggg tgatacaggg taaaagccgg cacgttgtat ttgacgatct
    26221 gggcagtgga tggattttac ccaaaactgc cgaaggtaaa cctgaaggcc gtttcacttt
    26281 ctccaaacat ccgaacatgg agggttacgg agcgctcagg agcaatgttg tagcacatcc
    26341 gtattattcc ggtcaactct acgttgggag tgaaaatgca ctttgggtaa gcaaggactt
    26401 tggaagtacc tttgatttgt tgtatgattt cggcagcaaa gtgaggtatt ttgatatcag
    26461 cacccaaaat ccggatgtga tatatgtaga tgtagctggc agtggatttt tcagaagcga
    26521 agacggtgga aaaactttta taaaaaaagt aagtattgcc ggttctaccg gtggaaacct
    26581 tcgctttgta atttcaccct acagcagcga tgtagtctat gccacactga cacacggtgt
    26641 ttgggacgag acaagaagtg aaatttataa aagtacaaat ggcggacaga catgggagaa
    26701 atggtcaaca tttggtacga gtattctaat aaagacaata gcaatacaac ccactaccga
    26761 tggcgaggat cttatatatg catttattga ttcctatgga tataattcag gtacagtaaa
    26821 ataccgcaag ggtaacggag catggataga ttttggaacc g
//
LOCUS       DAORKP010000010        26151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_54_length_26151_cov_10.774640, whole genome shotgun sequence.
ACCESSION   DAORKP010000010 DAORKP010000000
VERSION     DAORKP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26151
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_26151_cov_10.774640"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1409
                     /locus_tag="PLN75_01330"
     CDS             150..1409
                     /locus_tag="PLN75_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG02787.1"
                     /translation="MNTLEEIFAALKHNKLRTTMTGLSVSWGIFILIVLLGAGNGLQN
                     GVTSNFSNRATNTVQMWPGQTSLPYKGLKSARNLNFSEKELSTVDDNVAESDEETGII
                     DKSSTITFGNEYGSYRIKGVHPSYQKVFNLEFTARNGRFINNLDIEKSNKVIVLDKKI
                     EETLFKNKPALGQYVKVGSIVFKVVGVNTKKEGWDGSQAYIPFTTAQSIYNPDKKFNS
                     IAFTTKGLETKESNERFDKQLKTKMSQTMLFDPKDEKALWINNSQENYLQTMRIFKGI
                     NLFVTIVGIFTLIAGIVGVSNIMLVSVKERTREIGIRKAIGASPASILTSIVIESILI
                     TALFGYIGMMMGIGLTEVVNFVMEKSAAGQTNAGGEQMSVFKNPTVQIGYVIFSTVIL
                     IISGVIAGYMPARRATRIKPIEAMREE"
     gene            1530..2786
                     /locus_tag="PLN75_01335"
     CDS             1530..2786
                     /locus_tag="PLN75_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG02788.1"
                     /translation="MFDPDRWYEIWQAITHNKSRSVLTAFGIFWGMFMLVVMVGAANS
                     LKSNMMKEIEGFATNSCFVSDGVTSEPYKGFKKGRSWNIVNADLPVLVNKIPEMQHLS
                     PVLFGGRSTDKNVVRGEKSGSFNSKGVYANYNEIDRSKMKFGRFINSIDVAEQRKVCV
                     IGKRVYEVLFNNGENPVGKNIQLNGIYFQVIGVSGNSSGINIGGNAEETVILPFSTMQ
                     QAFNQGNIVHFLAITADPGVKVSEVEKAVKDELKRIHNISPTDKTAVWSLNLEDMFTM
                     FFYLQIGIGILVWIVGLGTLAAGGIGVSNIMLVTVRERTKEIGIKRALGATPANIISQ
                     IIIESIILTLIAGLAGMMFGVGILQVIGILFSQNAESMLKDPQISFTIAIGSLFVILI
                     VGSLAGLIPARRAVSIKAIEAIRDEN"
     gene            2940..4055
                     /locus_tag="PLN75_01340"
     CDS             2940..4055
                     /locus_tag="PLN75_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRG02789.1"
                     /translation="MKKVLKITLWVLLGLVVLGTFYFLYQKSKPKIIRYEIETVTSGS
                     IEKRTVATGKVEPRNEILIKPQMSGIISEVYKQAGDAVKAGDVIAKIKVVPDMVNLNA
                     SESRVQLAKLAYEQALTVYNRDKALFDKKVISKEEFEKSTLTLNNNKEELKTAQDNLS
                     LVREGISSRTAAYSNTLVRSTVTGTILDVPVKVGNSVIQSNNFNDGTTIATVANLNDM
                     LFVGKLDETEVGRIHTGMPMTITIGALQDHKLAANLEYIAPKGTEESGAIMFQMKASV
                     KIPSDIFVRAGYSANAEIMLEQAIDVLTIPESCLEFSNDSAFVYLLTKDEPQEFKKTV
                     VKTGLSDGINIQLTQGLKLGEKIRGAEIVEKKKENVN"
     gene            4069..5373
                     /locus_tag="PLN75_01345"
     CDS             4069..5373
                     /locus_tag="PLN75_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRG02790.1"
                     /translation="MKTKIITAIILFFCVLQISAQNRWTLPECIDTALANNRNIKKQK
                     LNYKSGEISYNQAKANRLPNLNASLGQNFNFGRSLAADNTYQNSNSQNTSFSLSSGVT
                     LFNGFRMKNTIEARQAELLAAGADVQKIEKDIILNVSGVFLQVLQNKEMLRNAENQLN
                     ITRENIARRKELIEAGKLAEGEIFEIQAQEAKEELARVQAENQLKLSLLDLSQVLELD
                     HFEDIDVAVPENLLENELALLSADEVYSSALDNRPEIKSAQHRLESSLKSVEIANAGK
                     LPSLSMGAQWGSGYYNMSNLPNNASFGKQFSNNMSTGIGLNLSIPIFNRYEVKNQVES
                     AKLNVESSKIDIENTKIELRKTIQQAYYNALAAKNRWDSSRKSVKANEESYRFASQKY
                     EVGRANQYELNLAKTNLSQSISEETQAKYEYVFRMKILELMK"
     gene            5444..5776
                     /locus_tag="PLN75_01350"
     CDS             5444..5776
                     /locus_tag="PLN75_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRG02791.1"
                     /translation="MNADNIKSQMRKGYLEYCILLILKKKPAYASDIIAELKAVKMIV
                     VEGTMYPLLTRLKNSELLDYRWEESTQGPPRKYYEMTAKGHAFLNELDIAWKEINEVV
                     DNLQQNLQ"
     gene            5815..7500
                     /locus_tag="PLN75_01355"
     CDS             5815..7500
                     /locus_tag="PLN75_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRG02792.1"
                     /translation="MKKTLTINLNNTVFHIDDDAYELLQSYLAEVSHHFKSETEKADI
                     MSDIEARIAELFSEKMDKQKNVITIEDVDTVITIMGKPSQFVDDDTDEESQQNAQEDA
                     SKKKTHKKYYRDVDNQLLSGVSAGLAAYLNWDVAIVRIIFVVLAFVTSGTFILIYLLM
                     WLIVPKAETTAQKLEMHGEDVNIESIKNKMVDAKEYLESDKFKQTATATGNRIWEIIR
                     VFLKILLTVTMAIVSIIGVILIATLILALILFLLEPEVITSISPQFFAIFGNTSPDKV
                     ILLLLALLFIIGCPIFALIYWTTGIVSNNKKPRSSSALWIALILWLAGIFMFIGTGAD
                     TLKKLNDSNITSYNSNSDNPEYTDEARTLADFTTIDASGAIEIELTQQADQSVSVRTL
                     REYLGNVKTEVADGILKIYSTDNLIKPTIKIKIGVDSLSKIEARGASKIEFTDTFSLK
                     KLNINLRGASNADIKVNSAQKLEFDVQGASKLDISGSADTLAIRGDGASNIEADDLGS
                     KVVRIEMNGASHAKVFASESFDGHAFGVSKIVVKGNPAKRTNEFNPGSSIHYE"
     gene            7523..8278
                     /locus_tag="PLN75_01360"
     CDS             7523..8278
                     /locus_tag="PLN75_01360"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRG02793.1"
                     /translation="MKLNLLISGLVLLCALPAISQKKEYTIVSEKRNVASFETIDVSG
                     RFVVMLYPDTKPEVTVMAAEEYIADVETVVENGVLKINMIDLTDNKNVSIVDGLRTKY
                     NDYLIRNPIEIHVGVTNFSKIYVKGVTTLETESMLKLQNLYIDINDASKANLDIEVEN
                     GFMAALSGASKINIKGKAMNADISASGASSFEAKDLMIRNAKISLSGASRAEIHASES
                     LDADLKGATKLICTGSPKNIRQNASRGSGIIVK"
     gene            complement(8353..11205)
                     /gene="gcvP"
                     /locus_tag="PLN75_01365"
     CDS             complement(8353..11205)
                     /gene="gcvP"
                     /locus_tag="PLN75_01365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRG02794.1"
                     /translation="MMNEFLKNRHIGISEEDETIMLKKIGLKSTDDLIEQTIPHSIRL
                     QKTLELPQPLTERQYAEHIAGLSNQNKLFTSYIGQGWYDTCTPAVIQRNIFENPSWYT
                     SYTPYQAEISQGRLEALLNFQTAVADFTGLPLANASLLDEATAGAEAATMAFNLRSRD
                     KIKSGANILFVDENVFPQTLAVIRTRAFVQGMELEVGDFENVKFTERHFGAIIQYPNS
                     NGSIEDYTGFVTNAHENNCIVAVAADILSLALLTPPGEWGADIVFGSTQRWGIPMFYG
                     GPSAAYFATREEFKRDIPGRIIGISKDVNGKYALRMALQTREQHIKREKATSNICTAQ
                     ALLATMAGMYNVYHGAEGIRRIALRIHSIASYVNEILEVYGYTQENENFFDTLKVTLP
                     DSVSLNSFRSIALENEVNFRYFETGEIGISFDETTELDDVNTLIDILATAAGNNAVFA
                     EPESITEFKSFDECFERKSDFLTHDVFRMYHTETDMMRYIKKLERRDISLTHSMISLG
                     SCTMKLNAASEMLPLSRPEFNAIHPLAPKDQTEGYHELITELEKYLCEITGLDACSLQ
                     PNSGAAGEFTGLLAIKSYLNSKGENHRNKLLIPASAHGTNPASAVQCGFIPVVVKCDE
                     KGNVEINDWNQKAESNADTLAGCMITYPSTHGIFETTIKDMCDTIHKFGGQVYMDGAN
                     MNAQVGLTNPGIIGADVCHLNLHKTFAIPHGGGGPGVGPICVAKHLAEFLPDLNFGIN
                     TVSSAPYGSAGVLAITYGYIRMLGEEGLTHSTKMAILNANYLAACLGDTYGIVYTGET
                     GRVGHELILECRNFKNTSGVSETDIAKRLMDYGYHAPTLSFPVHGTLMIEPTESESLA
                     ELDKFTDAMLKIYDEIKEIENGIADKTNNVLMNAPHPEYEIVGDDWNHPYSRQKAAYP
                     APWVAENKFWINVARVDNAYGDRNLVCACRPVSDWE"
     gene            complement(11438..14164)
                     /gene="ppdK"
                     /locus_tag="PLN75_01370"
     CDS             complement(11438..14164)
                     /gene="ppdK"
                     /locus_tag="PLN75_01370"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRG02795.1"
                     /translation="MSNVKRVYTFGNGKAEGKAEMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTDVCTEYYQLGKDQVVELLKPEVEAAIAGVEALMKSKFGDIENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDSVVEGMTRKTGNPRFVWDSYRRFVQMYGDVVLGMKPANKN
                     DHDPFELIIEEIKEEKGVHLDTELSVDDLKEMVARFKAAVKKQTGHDFPESAYEQLWG
                     AICSVFDSWMNDRAILYRQMENIPAEWGTAVNVQAMVFGNMGDTSATGVCFSRDAATG
                     EDQFIGEYLINAQGEDVVAGIRTPQQITKIGSQRWAELAGVSEAERLAKYPSMEEAMP
                     EIYAELDALQTKLENHYKDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAIDMLHQG
                     MIDEKTALLRVDPIKLDELLHPIFDKSALNSAKVIAKGLAASPGAATGRVVFNADDAA
                     EWAARKEKVIMVRVETSPEDLAGMAAAEGILTARGGMTSHAAVVARGMGKCCVSGAGT
                     LKIDYVSKQMSVDDLVVKEGDYISLNGSTGDIYLGQVQTKEAELDADFAELMEIAEKY
                     TRMSVRTNADTPEDAKRAREFGAKGIGLTRTEHMFFEGARIKAMREMILSSTTAGRKK
                     ALEKLLPYQRADFEGILEAMAGYGVTIRLLDPPLHEFVPHQQEMQKALAEEMGISIDA
                     VKQKVNELEEFNPMLGHRGCRLGITYPEITEMQTRAIIEAALNLKAKGVDARPEIMVP
                     LVGTVAELKNQADVIRATAEQVFAEKGDRIEYQIGTMIEIPRAALTADHIAKEAEFFS
                     FGTNDLTQMTLGFSRDDAGKFLPDYLKKGIFKVDPFTAIDQNGVGKLVEMGVEKGRQT
                     KPNLKVGICGEHGGEPSSVEFCHRAGLNYVSCSPFRVPIARLAAAQAAVKNA"
     gene            complement(14465..17272)
                     /gene="uvrA"
                     /locus_tag="PLN75_01375"
     CDS             complement(14465..17272)
                     /gene="uvrA"
                     /locus_tag="PLN75_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRG02796.1"
                     /translation="MPKQGNIIIKGARVHNLKNIDLEIPRNKLVVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSSYARQFLGRMSKPEVDYIKGIPPAIAIEQKVNSRNPRSTVG
                     TSTEIYDYIKLLYARIGKTYSPVSGEEVKKHETEDVVQAALTYPEETRLMVLAPVKPE
                     KGITTTERLNLFLQNGYSRMTIGNEVFPIDELLRNTSLLKGKEDIYLLIDRLTADRSL
                     AGVSRLTDSVETAFLEGKGVCALKVYNSDETKMLYFSKDFEADGINFELPNEHMFSFN
                     NPLGACPVCEGFGKIIGIDEDLVVPNKSLSIYQDAVVCWRGDVMSEWKRELIHNAEKF
                     DFPIHKPFIDLSDEQRELLWTGNSYFEGLNAFFKMLEANQYKIQYRVMLARFRGKTIC
                     PECHGTRLKKEAGYVKINGKSITELVLLPVSQLNLFFENIVLDEHDSLAAKRLLIEIK
                     NRIKFLLNVGLDYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHSR
                     DTHLLIGVLKQLRDLGNTVVVVEHDEEIMRAADYIIDMGPGAGRAGGNVVWEGNTDEI
                     SLESQSEKIQNSYTLKYLTQLENINIPAHRRRWNNYIEVHGARENNLKGINVKFPLSV
                     MTVVTGVSGSGKSSLVKNVFYAALKKYYGGVADKTGQFGSLSGSMHLVKDIEFVDQNP
                     IGRSTRSNPVTYIKAYDEIRKLFSEQQLARQMGFAASFFSFNTPGGRCEECQGEGSVT
                     VEMQFMADLVLECEHCHGQRFKSDVLEVNYHGKNIYDVLNMTVNQAIEFFSEGHGNTE
                     KRIVKRLKPLQEVGIGYVKLGQSSSTLSGGESQRVKLASFLANEKPEPTIFVFDEPTT
                     GLHFHDIKTLLKALDALIAKGHTVVIIEHNLEVIKCADHIIDIGPEGGEKGGNVVFEG
                     TPEALIDCECSYTGKFLKSNI"
     gene            17527..18591
                     /locus_tag="PLN75_01380"
     CDS             17527..18591
                     /locus_tag="PLN75_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HRG02797.1"
                     /translation="MDTMTLYWTIFIALFFIVFFIDLYVTDHRKGEISVKTSLKWTGV
                     WISIALLFGASLFFFFPQNPDTPGNTAHVMGVKFISGYLTEYSLSVDNLFVFIMIFSM
                     MGVAAHNQPKLLKMGIILSIILRILFILVGMELVERFHWIIYLFGLILVWTAYKMAFQ
                     DSEDSVDPKSNFLYKGASKIYPVDPDTHSTKFFTKHNGTRYITMNLLVLLVIGSTDVL
                     FAVDSIPAIIGVIKEGASGILTLSEENFLAITSNVFAVMGLISLFFALKGIMGMFRFL
                     KHGVSFILFFIGAKMLLGAVPAVAQFFASHSWVSLAVIVFTLAFSILLSMVIAEKKEN
                     TELKAELELEKKEDERVDEV"
     gene            18644..19552
                     /locus_tag="PLN75_01385"
     CDS             18644..19552
                     /locus_tag="PLN75_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRG02798.1"
                     /translation="MQNLSEKTELKPQNPVIDWIIRMVKGVIIGIGFILPGLSGGVLA
                     VIFGLYDKILKFLANIKEDFIKNVLFFIPVGIGMAIGIFLFSVVVEKAFGKYAAIFIC
                     LFVGFVIGTFPSLYRTAGKQGRKTKDILIMLISALVLFGLMMLGEKQLMDVAPGILSW
                     LGSGALISLGLIVPGMSPSNFLIYFGLYDKMASGIKDLDLSVIIPLIVGAVICVLLFA
                     KLAAHLFRKYYSGMYHFILGMVIGSSVAIFPAIVFPALKPEVLSAAGLSIAGAVLLCI
                     AMLIAGTVASYLFSKVEEKHPREDIF"
     gene            19630..20781
                     /locus_tag="PLN75_01390"
     CDS             19630..20781
                     /locus_tag="PLN75_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRG02799.1"
                     /translation="MELSQLFPAIAGMTWQNLVMIAVGLTLIYLAIKKQYEPTLLLPM
                     GFGAILVNIPFTAALTQIDPATGKALEGALDVFFKAGIATEVFPLLIFVAIGAMIDFS
                     PLFQRPSLLLFGAAAQFGIFVTMVFATAVGFNLKEAASIGIIGAADGPTSIIVSSILA
                     PNLLGPISVAAYSYMALVPIIQPPVVRLLTSRKERLMRMTSDSNKKVSKTALILFPIM
                     VTILAGLIAPSSLALIGFLMFGNLIRECGVLNSLSQTAQKELANLVTILLGITIASTM
                     TGERFVTIDTIKILGLGIFAFVFDTAGGVLFAKVMNLFLKDEKKINPMVGACGISAFP
                     MSARVIHGMGQKEDPHNFLLMPAISANVGGQIGSVIVGGLILMLVPLFA"
     gene            20795..20992
                     /locus_tag="PLN75_01395"
     CDS             20795..20992
                     /locus_tag="PLN75_01395"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRG02800.1"
                     /translation="MSPDFYKALEISGIGIAGVFIFMTVFYFVIMGLDKFFPNEKKTK
                     QIAEAPKQEYKPVEFSDDEFE"
     gene            21184..22971
                     /gene="lepA"
                     /locus_tag="PLN75_01400"
     CDS             21184..22971
                     /gene="lepA"
                     /locus_tag="PLN75_01400"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRG02801.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRLLEFTQTVSGKDMQAQVLDNM
                     DLERERGITIKSHAIQMKYACQDEIYTLNLIDTPGHVDFSYEVSRSIAACEGALLIID
                     ATQGIQAQTISNLYMAIEHDLEIIPVMNKMDMDSAMPEEVEDQIVDLLGCKPEEIIRA
                     SGKTGMGIEEILAAIVERIPAPKGEPDAPLQALIFDSVFNPFRGIIAYFKIENGTIKK
                     GDLVKFVNTGKEYNADEIGVLKLDMSPADTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCEKAISGFEEVKPMVFAGVYPIEAEDFEDLRSSLEKLQLNDASLTFEPESSVALG
                     FGFRCGFLGLLHMEIIQERLDREFDMNVITTVPNVSYKVHTKKGELLEVHNPAGLPDI
                     MAIDTIEEPYIRASVITRTDYIGQIMTLCLGKRGELIKQEYVSGNRIEIIYDLPLGEI
                     VFDFYDKLKSISKGYASFDYHMNGWRPSKLVKLDILLNGESVDALSSLIHFDNAVSLG
                     RRMCEKLSELIPRQQFDIAVQAAIGAKIIARETIKAVRKDVTAKCYGGDVTRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFLAVLKLD"
     gene            23089..24390
                     /gene="nhaD"
                     /locus_tag="PLN75_01405"
     CDS             23089..24390
                     /gene="nhaD"
                     /locus_tag="PLN75_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRG02802.1"
                     /translation="MITIIIILFVIGYAAIALEHNIKINKTASALITGVVLWVVYILT
                     QSDTHLVNEELLKHLGDISSILFFLMGAMTIVELIDTYNGFEVITERITQTKKVSLLW
                     IIGILTFFLSAVLDNLTTTIVMVSLLRKLIKDHETRLFYVGIVVIAANAGGAWSPIGD
                     VTTTMLWIGGQISAFNIMARLFAPSFISLAIPLGVLSLTMKGRVERPFLATETYKELS
                     RRQQSIILILGLSMLIMVPIFKTLTHLPPFMGMMLGLGVLWIVTDIMHRNHTEDQPEN
                     GKRRTVTDVLTRIDLPSILFFLGILLAVASLEVSGILHTLSQFLDEKIGNLNFIVIAI
                     GLASAIIDNVPLVAATQGMYDLAQFPMDHYLWEFLAYCAGTGGSILIIGSAAGVAAMG
                     MEKINFFWYVKKISWLALLGYLAGAAFYIVEYYFSHGAFYH"
     gene            24481..25803
                     /locus_tag="PLN75_01410"
     CDS             24481..25803
                     /locus_tag="PLN75_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRG02803.1"
                     /translation="MYSNKKIFKVTYPIFLTLIAQNIVNVTDTAFLGRVSEVALGASA
                     IGGVFYIAIYFVGFGFSQGAQILIARRNGEKNFGEIGPIFNNALIFNFLIATAIFILS
                     AIFIPDLIRFLVHSDHIYQASIEFLNWRIYGFFFSFLNVVFRAFFVGITRTKVLTVSA
                     VITALTNIVLDYLLIFGKFGFPEWGIAGAAIASAIAEFTTLIYFIIVTLKQDDKHIFH
                     LFVFRKIDFHTIGKILSLSVFIMFQYFISISTWFMFFIFIERLGERPLAVTNIGRSLY
                     ILLMIPGSALSTTVNTLVSNMIGEGHKEQVIPFMMKILKIVLVILLPLMFFTFAFPEL
                     FARIYTNNSDLVSATIPTLRVVSVAMVFCGVGSILFSVISGTGNTKSAFIIEFLTLFF
                     YIVYVYYTAVILQTSVEIVWMSEFVYWTVIGLMAYWYLMKGNWQKKEI"
     gene            25967..>26151
                     /locus_tag="PLN75_01415"
     CDS             25967..>26151
                     /locus_tag="PLN75_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02804.1"
                     /translation="MKTHKSNIFRFGEDFLTIGTALKLARNNIKGVLTPETISRVQAN
                     RDVVLKIVHKKKVVYGI"
BASE COUNT         7572 a         5569 c         5567 g         7443 t
ORIGIN      
        1 ctgaataaaa ccggtattac caccgtcatt gttacacacg aacagtcggt agcagacact
       61 accgaccgca ttatacacat taaagacggt ctgatagaaa atcagtatca gactaaaaaa
      121 agtctaatca cacagtatta aaaattatca tgaatacgct tgaagagata tttgccgctc
      181 ttaaacacaa taaactccgc actaccatga ccggcctgtc ggtttcgtgg ggaattttca
      241 ttctcatagt tctgctcggt gcaggcaatg gattgcaaaa cggagtaaca agcaatttta
      301 gcaacagagc caccaatacg gttcagatgt ggcccggaca gacttcattg ccatataaag
      361 gacttaaatc ggcccgaaac cttaattttt ccgaaaaaga attgagtacc gttgacgaca
      421 atgtggcaga aagcgatgaa gaaacgggaa ttatcgataa gagttccacc attacatttg
      481 gtaacgaata cggctcttac aggataaaag gtgtacatcc gagttatcag aaagtattta
      541 atcttgaatt cacagcccga aacggacggt ttatcaacaa cctggacatt gaaaaaagca
      601 ataaagtaat agtacttgac aagaaaatag aggaaacact attcaaaaac aagccggcac
      661 tcggacagta tgtgaaagtg ggttccatcg tattcaaagt ggtgggtgtc aacaccaaaa
      721 aagagggatg ggatggttcc caggcatata ttcccttcac cacagctcaa tctatttaca
      781 atccggataa aaaattcaac tccatagcat tcaccacaaa agggcttgaa accaaagaat
      841 cgaatgaacg atttgacaaa cagcttaaaa ccaaaatgtc gcaaaccatg ctgtttgatc
      901 cgaaagacga aaaagcgctc tggataaaca actcgcagga aaattacctg caaaccatgc
      961 gaattttcaa aggaattaac ctgtttgtaa ctatagttgg gattttcaca ctcatagccg
     1021 gtattgtggg tgtaagcaat ataatgcttg tttcggtaaa ggagcgcacg cgcgaaatag
     1081 gcatcagaaa ggctatcgga gcatcacccg cttccatttt gacttctata gtgattgaat
     1141 caatcctgat tactgccctt tttggctata tcgggatgat gatgggtatc ggattaactg
     1201 aagttgtaaa cttcgtgatg gaaaaaagcg ctgccggcca aacgaacgcc ggaggtgagc
     1261 aaatgtcggt cttcaaaaat ccaactgtac aaatcggtta cgtgatattc tcaacggtga
     1321 tactcattat ttcgggtgtg atagccggat acatgcctgc ccgaagagca acccgcatta
     1381 aaccgattga agcgatgaga gaagaataac cccaaaaccc tgaagtgtag tcttatatac
     1441 taattatgta ataaaaaata atgcaaaaca gcaaaacaac aaataacgaa aagatcaaaa
     1501 cttccccttc tggaggtcgg ggggcacgta ttttcgatcc ggaccgctgg tatgaaatct
     1561 ggcaagccat cacgcacaac aaatcaagaa gtgtgcttac cgctttcggt atattctggg
     1621 gtatgtttat gctggtagtg atggtgggtg cagccaactc gctgaaatcg aacatgatga
     1681 aagaaattga gggttttgcc acaaactcct gcttcgtaag cgacggggta acctccgaac
     1741 cttacaaagg attcaaaaag ggacgatcct ggaatattgt caacgcagac ctgccggtac
     1801 tcgtgaataa aattcctgaa atgcagcatc tttcacccgt cctatttggc ggccggtcca
     1861 ccgataaaaa cgtagtaaga ggtgaaaaat caggctcgtt caattcaaag ggagtttatg
     1921 ccaactacaa cgaaatagac cggagcaaaa tgaagttcgg aagattcatc aactctatcg
     1981 acgtggcaga acagcgtaaa gtatgtgtaa taggaaaaag ggtgtatgaa gttctcttca
     2041 acaacggtga aaatccggtg ggaaaaaaca tacaactcaa cggaatctat tttcaggtaa
     2101 tcggagtttc gggcaattca tcgggcatca acatcggagg aaatgccgaa gaaactgtca
     2161 ttttgccatt ctctaccatg cagcaggcat tcaaccaggg aaacatcgtg cattttctgg
     2221 ccattacggc cgatccgggt gtaaaagtga gtgaagtgga aaaagcagtg aaagatgagc
     2281 ttaaacgaat tcacaacata tctcctaccg acaagacagc cgtatggtcg ttgaacctgg
     2341 aggatatgtt taccatgttt ttctaccttc agattggtat cggaattctg gtgtggatag
     2401 tgggtttggg tacgctggct gccggaggaa taggcgtaag caacattatg ctggtcaccg
     2461 tacgcgaacg gacaaaagaa atcgggataa aaagagcact cggagctact ccggccaata
     2521 tcatctcaca gataattatt gaaagtatca tcctgacact gattgccgga ttagccggga
     2581 tgatgttcgg agtaggtatc ttacaggtta tcggcatatt attcagtcag aatgcagagt
     2641 ccatgctgaa agatcctcag ataagtttca caatagccat cggatcgctc ttcgtaatac
     2701 tgattgtagg tagtctggca ggactcattc cggccaggcg ggcagtaagc atcaaagcca
     2761 tcgaagctat cagagatgaa aattaaggga aacaactctc tgcggtcaga atttattaaa
     2821 aaaccgaatc atacagattt tttatttaga gaaaaagtaa ttttaaaaag aaataaagta
     2881 ttaaccattt aaaggcctga aaattcactg ccaggtggaa acagggaaaa taaaaattta
     2941 tgaaaaaagt attaaaaatc acattgtggg tacttctcgg attagttgta ctgggtactt
     3001 tctacttctt atatcagaaa tccaaaccaa aaatcatccg ttatgaaata gaaacagtga
     3061 ccagtggatc tatcgaaaaa agaacagtag ctaccggaaa agtagaaccc cgaaacgaaa
     3121 ttctgataaa gccacagatg tccgggatta tttcggaagt ttacaagcag gccggcgatg
     3181 ctgttaaagc aggtgatgtc attgctaaaa taaaagtggt gccggatatg gtcaatctga
     3241 acgcatccga atcgcgcgta caactcgcca aactggctta cgaacaagca cttaccgttt
     3301 acaatcgcga caaggcactc tttgataaaa aggtaatatc aaaggaagaa ttcgaaaaat
     3361 cgacgctgac attgaacaac aacaaagaag aactgaaaac cgctcaggac aatctgagtc
     3421 ttgtacgcga aggcatttcg agccgaacgg ccgcttacag caacacgctg gtgagatcaa
     3481 ccgtcaccgg aaccattctt gatgtgccgg tgaaagtcgg aaattcggtc attcagtcga
     3541 ataatttcaa cgacggaacc accattgcca ccgtagccaa tctgaacgac atgctattcg
     3601 tgggcaaact ggacgaaacc gaagtggggc gcattcatac cgggatgccc atgacaatca
     3661 ctatcggggc gcttcaggat cacaaactgg cagccaacct ggagtacatt gctccgaaag
     3721 gaacggaaga aagcggagct atcatgtttc agatgaaagc ctcggtaaaa attccatcgg
     3781 atatatttgt gcgtgctggc tatagcgcga atgccgaaat catgctcgaa caggccattg
     3841 acgtgctcac catccccgaa agttgtctgg aattcagcaa cgacagcgcc tttgtgtatc
     3901 tgctcaccaa agacgaaccg caggaattta agaaaacagt ggtgaaaacc ggtctttcag
     3961 atggcatcaa catccagcta acccagggtc tgaaactggg agaaaaaata cggggagccg
     4021 aaattgtgga aaagaaaaag gaaaacgtta actaaaaaac tcccgaaaat gaaaacaaaa
     4081 atcataacgg caattatact gtttttctgc gtgttacaga tttcggcaca gaaccggtgg
     4141 acactccctg aatgtattga cacggctctg gccaacaacc gtaacatcaa gaaacaaaaa
     4201 ctgaattata aatccggcga aataagctac aatcaggcaa aagccaaccg tttgcccaac
     4261 ctgaatgcat ctttgggaca aaacttcaac tttggccgct cgctggctgc cgacaataca
     4321 tatcaaaatt cgaattcaca aaacacgtcg ttttctcttt catcaggagt gacacttttc
     4381 aacggattca ggatgaaaaa caccatcgaa gcacgtcagg cagaactgct ggcagccgga
     4441 gccgatgttc agaaaataga aaaagacatc attctgaatg tatcaggtgt gtttctgcag
     4501 gttcttcaaa acaaggaaat gcttcggaat gcagaaaacc agctgaacat aacccgcgaa
     4561 aacatagcaa gacgaaaaga gctgatagaa gccggaaaac tggcagaggg tgaaattttc
     4621 gaaattcagg ctcaggaagc taaagaagaa ctcgcacgcg tacaggctga aaaccagctg
     4681 aagctatcac tgctcgatct atcgcaagta ctcgaactgg accattttga agatattgat
     4741 gtggctgtac ctgaaaattt gctcgaaaac gagctggcat tgctttcggc cgatgaggtt
     4801 tacagcagtg ccctggataa ccgtccggaa ataaaatcgg cccagcaccg attggaaagc
     4861 agcctgaaat cggtggaaat tgccaatgcc ggaaaattac catcactatc tatgggagca
     4921 caatggggct caggctatta caacatgagc aacttaccta acaatgcttc cttcggcaaa
     4981 caattttcaa acaatatgag cacgggtatc ggattaaacc tttccatccc tattttcaat
     5041 cgttatgaag tgaaaaatca ggtggaaagt gccaaactta acgttgaaag ctcgaaaatc
     5101 gatattgaaa acacaaaaat agaacttcgg aaaacaatac agcaggctta ttacaacgct
     5161 ttggcagcaa aaaaccgctg ggattcgagc cgcaaatcgg tgaaagcaaa cgaagaatca
     5221 taccgttttg ccagtcagaa atatgaagta ggcagagcca atcaatacga acttaacctc
     5281 gctaaaacca atttatcgca gtccatttcc gaagagacac aagccaaata tgaatacgtt
     5341 ttcagaatga aaatactgga attgatgaaa taatttattc agaaaaagta ctacaagaca
     5401 atcaagtctt gtagccttct taaaaaaaca atttacaata actatgaacg cagacaacat
     5461 taaatcacaa atgcggaaag gttatctgga atactgcatt ctgcttattc tgaaaaagaa
     5521 acctgcatac gcttcggaca tcattgccga actcaaagct gtaaagatga tagtagtgga
     5581 aggcaccatg tatccgcttc ttacacgtct gaaaaacagc gaattgctcg attaccgctg
     5641 ggaagaatcc acccagggtc cgccccggaa atactacgag atgactgcca aaggccatgc
     5701 atttctcaac gaactggata tagcctggaa ggaaataaac gaagtagtag ataatttaca
     5761 acagaattta caataaaaaa tcggtcagaa ctaacattaa aaaaacacaa acagatgaaa
     5821 aagacattga caatcaatct gaacaataca gttttccaca tcgatgatga tgcgtacgaa
     5881 ctcctgcagt cgtacctggc tgaagttagt catcatttca agtcagaaac agaaaaagcg
     5941 gacatcatga gcgatattga agcccggatt gcagaacttt tttcggaaaa aatggacaaa
     6001 cagaaaaacg taatcaccat tgaggatgtg gatacggtca ttacgattat gggaaaaccc
     6061 agtcagttcg tagatgatga cacagatgaa gaatcacagc aaaacgctca ggaagacgct
     6121 tcgaaaaaga aaacccacaa aaagtattac cgcgacgttg acaatcagct gcttagcggt
     6181 gtgtcagccg gattagccgc atatctcaac tgggacgtgg cgatcgtacg tatcattttt
     6241 gtggtattgg ctttcgttac ttccggtacg tttatactta tctatctgct catgtggctg
     6301 atagtaccca aagctgaaac aaccgcccag aaactcgaaa tgcacggtga agatgtaaat
     6361 attgaatcca tcaaaaacaa gatggttgac gccaaagaat acctggagag cgataaattc
     6421 aaacaaaccg ctaccgccac cggaaacaga atatgggaaa taatccgggt attcctgaaa
     6481 atactactca ctgtcacaat ggctattgta agtataattg gtgtaatctt aattgctacg
     6541 ctgattcttg ctctcattct tttcttactc gaaccggagg taattacaag catttctcca
     6601 caattctttg ccattttcgg caacacatca cccgataaag tcatcctgct gcttttagcg
     6661 ctgctgttta tcatcggatg tcctatattc gcactgattt actggactac cggaattgtt
     6721 tccaacaaca aaaaacccag atcgtcctcg gcattgtgga ttgctttaat actctggctg
     6781 gcaggcatat ttatgtttat cggcacagga gccgatactc ttaaaaaact gaacgacagc
     6841 aatataactt cgtacaactc caactcagac aaccctgaat acacagatga agcacgcact
     6901 ctggccgatt tcactaccat tgatgcttcc ggagctattg aaatagaact tacccaacag
     6961 gcagatcaat ctgtatcggt aagaacgctc cgggaatacc ttggaaacgt aaaaaccgaa
     7021 gtagctgatg gaatactgaa aatctattca accgataacc tgataaaacc tactataaaa
     7081 attaaaattg gagtagattc actcagcaag atagaagccc gcggagcttc aaaaattgaa
     7141 ttcacggata ccttttcgct aaaaaaatta aatattaacc ttcgcggagc aagcaatgcc
     7201 gatataaaag taaattcggc acagaaattg gagtttgacg tacaaggtgc aagtaaactt
     7261 gacatcagcg gctctgcaga caccctggct atcagaggtg atggcgccag caatatcgaa
     7321 gccgatgatc tgggctcaaa agtagtgagg atagaaatga acggagcaag ccatgccaaa
     7381 gtttttgctt ccgaatcatt cgacggacat gcatttggag tcagtaaaat agttgtaaaa
     7441 ggtaatccgg caaaaagaac caatgaattc aatcccggat cgagtattca ttacgagtaa
     7501 aaatttttca aaaaaaaatg tcatgaaact aaatctgtta atatcgggtc tggtactgct
     7561 atgtgcacta cctgccattt cacaaaaaaa agaatacaca atcgtatccg aaaaaagaaa
     7621 tgtagcttct ttcgaaacga tagatgtatc cggaagattt gtggtaatgc tctatccgga
     7681 cacaaaaccg gaagtcaccg taatggctgc cgaagagtat attgccgacg ttgaaactgt
     7741 ggtagagaac ggtgtattga aaatcaacat gatagacctt accgacaaca aaaatgtaag
     7801 tattgtggac ggtttgagaa ccaaatacaa cgattatctg atacgtaatc ccatcgaaat
     7861 acacgtcgga gtgaccaatt tcagcaaaat ctacgtaaaa ggagttacga ctttggaaac
     7921 ggaatcgatg ctcaaattac agaatttata catcgatatc aacgatgcct caaaagccaa
     7981 tctggacatt gaagtcgaaa acgggtttat ggcagcactc tcaggtgcat ccaaaataaa
     8041 catcaaaggc aaagcaatga atgccgacat cagtgccagc ggcgcatctt cattcgaggc
     8101 gaaagacctc atgatccgga atgccaaaat atcactgtcg ggagcaagca gggccgaaat
     8161 tcatgcctct gagagtctgg atgccgactt gaaaggtgct acaaaactta tctgtacagg
     8221 aagccccaaa aacatcagac aaaacgcttc aagaggctcc ggtattatcg taaaataaat
     8281 cagagtatca gcaaacagaa tgccggaaag taaaaatact tccggcattt tgtttggtgt
     8341 gattatggaa ttctattccc aatcgcttac agggcggcag gcacacacca gatttcggtc
     8401 gccgtaagca ttatctaccc ttgcaacgtt tatccagaac ttgttttcag ccacccacgg
     8461 agccggatat gccgcttttt gcctagaata tggatgattc cagtcatctc ccactatttc
     8521 gtactcggga tgcggggcat tcatcaacac gttgttggtt ttatcggcaa tgccattttc
     8581 aatctctttt atttcatcgt agattttcag catagcatcg gtaaatttgt ctaattcggc
     8641 cagcgactca ctttcagtag gttctatcat cagtgtgcca tgtacgggga atgataaagt
     8701 gggcgcatga tagccataat ccatcagtcg ttttgcaata tctgtctcgc tcacaccgga
     8761 agtatttttg aaattccggc attcaagaat caactcatgc cctacccgtc cggtttcgcc
     8821 ggtataaaca atgccgtatg tgtctccaag gcaggctgcg aggtaattag cattgagaat
     8881 ggccattttt gtggaatgtg tcagtccttc ttcaccaagc atgcgtatgt atccataggt
     8941 gattgccaat acgccggcac ttccgtaagg tgcagacgaa actgtattaa ttccaaaatt
     9001 taaatcaggc agaaattcgg ccagatgttt tgccacgcaa atagggccta cacccggtcc
     9061 gcctcctccg tgaggtattg caaatgtttt atgcagattc aggtgacaaa catcggctcc
     9121 gattataccc ggattagtaa gtccaacctg tgcgttcata ttggctccat ccatatatac
     9181 ctgtccgccg aatttgtgaa ttgtatcaca catatccttt attgttgttt cgaaaatgcc
     9241 gtgtgtggaa ggataggtga tcatacaacc ggcaagcgta tcagcattag attctgcttt
     9301 ttgattccag tcatttatct ccacgtttcc tttttcgtcg catttcacca ctacagggat
     9361 aaatccgcac tgaactgcgc ttgcgggatt ggtaccgtga gctgaagcag gaatgagcag
     9421 cttgtttctg tgattttctc cctttgagtt gaggtatgat ttgatagcga gcaatccggt
     9481 gaactctccg gcagctcccg aattcggttg caggctgcat gcatccagcc cggtaatttc
     9541 gcacaggtat ttttcaagtt cggtgatcag ttcatgatac ccttcagtct gatctttggg
     9601 tgcaagcgga tgaatcgcgt taaattccgg gcggcttaac ggcaacattt ccgatgctgc
     9661 attcagtttc atggtgcagg aacccagcga aatcatcgaa tgagtgaggg aaatatcacg
     9721 tcgttcaagt tttttgatgt aacgcatcat atctgtttcg gtatgataca tccggaaaac
     9781 atcgtgggtg agaaagtccg attttctttc gaaacattcg tcgaatgatt tgaattctgt
     9841 aatactttcg ggttcggcaa acacggcatt attgcctgca gctgttgcca gaatgtctat
     9901 cagcgtgttc acatcgtcca gttcggtcgt ttcatcgaag ctgattccga tttcacccgt
     9961 ttcgaaatag cggaagttga cttcattttc caaagcgatt gatctgaaac tgttcaaact
    10021 taccgagtcg ggcaaagtga ccttgagtgt atcaaagaaa ttttcgtttt cttgtgtgta
    10081 tccgtacact tcgagtattt cattcacata gctggctatg gaatgaatcc ggagagcaat
    10141 gcggcgaata ccttcggcac cgtgatacac gttgtacatt ccggccatgg tagccagcag
    10201 agcctgtgcg gtacagatat ttgaagtggc tttttctcgc ttgatgtgtt gctcgcgggt
    10261 ttgcagcgcc attcggagtg cgtattttcc gttcacatct ttgcttatac ctattattct
    10321 gccgggaata tcgcgtttga attcttctcg tgtggcaaaa taagcagctg atggtccgcc
    10381 atagaacatg gggatacccc agcgctgggt ggaaccgaaa acgatatcgg ctccccactc
    10441 gcccggaggt gtgagcagcg ccagactcag aatatcggct gctacggcta caatgcaatt
    10501 gttctcgtgg gcattagtta caaaaccggt ataatcctcg atgctgccat tggaattagg
    10561 atactgaatg atggctccga aatggcgttc ggtgaatttt acattctcga aatcacccac
    10621 ttcaagttcc attccctgca caaatgcacg tgtacggatt acagccagtg tttgaggaaa
    10681 cacattttca tctacgaaaa ggatgttagc tcctgatttt attttatcgc gacttcttag
    10741 attaaaagcc attgtggcgg cttctgctcc ggctgtggct tcgtcaagca atgaggcatt
    10801 ggccagtggc agaccggtaa agtcggccac tgcggtttgg aaattgagca atgcttcaag
    10861 tcggccctga gatatttctg cctgataggg cgtataggat gtataccagg agggattttc
    10921 gaatatattt cgctgaatta cggctggtgt gcacgtatca taccatccct gtccgatgta
    10981 cgatgtgaaa agtttgtttt gattggaaag tccggctatg tgttcggcgt attgtctttc
    11041 tgtgagcggc tgtggtaatt caagcgtttt ctgaagacgg atggaatgcg gaatggtttg
    11101 ttcgatgagg tcatctgtac ttttcaatcc gatttttttc agcatgatgg tttcatcctc
    11161 ttcagagata cctatgtgcc tgtttttcaa gaattcgttc ataataatag ttctatagat
    11221 tttgatttca aagataattc tttttccatt cttacagaaa atatgtttga gtaaaaattc
    11281 ataaaaattc tgtttttttg cctgataccc tttttaacag actgtcagtc attttttttc
    11341 tgaacgactg caattttatc ctgaggatga aaaattaatg cataaaaaaa agccgtcggt
    11401 tgacagcttt tttctaaatc aggcttttat ggatttacta tgcattttta acagctgcct
    11461 gagctgctgc gaggcgtgca ataggcacac ggaaaggtga gcaacttaca tagttgagac
    11521 ctgcacggtg gcagaattca actgaagatg gttcgccgcc atgttcacca caaataccaa
    11581 ctttgaggtt tggttttgtc tgacgacctt tttcaacacc catttctaca agtttaccta
    11641 caccattctg atcgatagca gtaaacggat caactttgaa aattcctttt ttcagataat
    11701 cgggtaagaa tttaccggca tcgtcgcgtg agaaaccaag tgtcatttga gttaagtcgt
    11761 ttgtaccaaa cgagaagaat tctgcttctt tggctatatg atcggcagta agcgctgcac
    11821 gcggtatttc aatcattgta ccgatttgat attcaatgcg gtcacctttt tcagcaaata
    11881 cctgttctgc tgtagctctg ataacatcag cctgattttt caattcagca acagtaccca
    11941 ccagcggaac cataatttca ggacgggcat ccacaccttt tgctttcaga tttagagcag
    12001 cctcaataat tgcacgggtt tgcatttcag taatttccgg ataagtaata cccaatcgac
    12061 aaccacgatg tccgagcatt ggattgaatt cttcgagttc gtttactttt tgtttcactg
    12121 catcgatact gattcccatt tcttcggcaa gcgctttttg catttcctgc tgatgaggaa
    12181 caaattcatg caaaggtgga tcgagcagac gaatggtaac accataacct gccatagctt
    12241 ccagaattcc ttcgaagtcg gctctctggt aaggcagcaa tttttcgagg gcttttttgc
    12301 gtccggctgt ggttgacgaa agaatcattt cgcgcatggc tttgatacgt gcaccttcga
    12361 agaacatgtg ttccgtacgg gtaagtccga taccttttgc accgaattct ctggcacgtt
    12421 ttgcatcttc gggcgtatca gcattggtac gcacgctcat gcgggtatat ttttcggcaa
    12481 tttccatcaa ttcggcaaaa tcggcatcca gttcagcttc tttagtttgc acctgaccga
    12541 gataaatgtc gcctgtggaa ccattgagcg aaatataatc gccttcttta accactaaat
    12601 cgtcaacact catttgtttg ctaacgtagt caattttaag tgttccggcg ccggaaacac
    12661 aacattttcc cataccacgt gcaacaactg cagcgtgcga agtcataccg ccacgggcag
    12721 taagaatacc ttcggcagca gccataccgg caaggtcttc aggtgaagtt tcaacacgca
    12781 ccatgattac cttttccttg cgtgcagccc actcggcagc atcatcggca ttgaaaacta
    12841 cgcgaccggt agcagcaccg ggagaagcag ccaaaccttt tgcaatcact tttgcagaat
    12901 tcaatgcaga tttgtcaaaa atggggtgaa gaagttcatc cagtttaata ggatcaacac
    12961 gcaaaagagc agttttttcg tcaatcatgc cctgatgcag catatcgatg gcaattttca
    13021 ccatagcagc accggtacgt ttaccgttac gtgtctgaag gaaccaaagt tttccttcct
    13081 gaacggtaaa ttccatgtcc tgcatatctt tgtagtgatt ttcgagcttg gtttgcagcg
    13141 catcaagttc ggcataaatt tcgggcattg cttcttccat cgaaggatat ttggcaagac
    13201 gctctgcttc tgaaacaccg gccagttcag cccagcgttg tgagcctatt tttgtaattt
    13261 gctgtggtgt gcgaataccg gcaaccacat cttcaccctg cgcattgata agatattcac
    13321 cgataaactg gtcttcgcct gtagcagcat cgcggctgaa gcaaacacct gtagccgaag
    13381 tatcgcccat gtttccgaaa accattgcct gaacgttaac tgctgttccc cattcggcag
    13441 gaatattttc catctgacgg taaagaatag cgcgatcgtt catccacgaa tcaaatactg
    13501 agcagatagc tccccaaagt tgctcgtaag ccgattcggg aaaatcgtgt ccggtttgtt
    13561 ttttcacggc tgctttgaaa cgtgcaacca tttctttcaa atcgtctaca gacaattctg
    13621 tatcgagatg tacacctttt tcttctttta tttcttcaat aatcagttca aaaggatcgt
    13681 gatcgttttt atttgcaggt ttcataccta aaaccacgtc accatacatt tgtacaaaac
    13741 gacggtacga atcccacaca aaacgcggat tacctgtttt gcgggtcatt ccttccacca
    13801 cactgtcatt taatcccagg ttcagaattg tatccatcat accgggcata gaagcacgag
    13861 ctcctgaacg taccgaaact aacaatggat tttcaatatc gccgaatttt gatttcatga
    13921 gagcttctac accggctatg gcggcttcaa cttcaggctt caatagttcc acaacctgat
    13981 ctttgccaag ttgatagtat tccgtacaga catctgtagt aatggtaaat ccgggaggta
    14041 caggcactcc gatcagattc atttcggcaa ggtttgcccc cttgccacca agcagatttt
    14101 tcatttctgc cttaccttcg gcttttccgt taccaaaggt gtaaactctt tttacgttgc
    14161 tcataaattt attatttgtt ttttaatgta atatgctgat gatgaaaata taaataaaga
    14221 atctgtaaaa tgtaacagtg gaaaaatcaa ttgcttgtaa ataagtgaca aagataattt
    14281 ttttatttaa cattataaca tttacaggaa aaaagggaat caattttgtg cattttaatt
    14341 ggttatttat aacaacaaaa tgtccggttt agttcgtttt tttgctgaaa aaacaggaaa
    14401 ttatcatcaa ttactttttc atttttggaa ttaactactt ttacttgata tgaatcaaaa
    14461 aaagctaaat attactttta aggaatttcc cggtgtagga gcattcacag tcaatcaatg
    14521 cttccggtgt tccttcaaat accacatttc cacccttctc acctccttcg gggccgatat
    14581 cgatgatgtg atcggcacat tttataactt ccaggttatg ttcgatgatg actacggtat
    14641 gtcctttggc aatcaaagca tcaagggctt tcaacagggt ttttatgtcg tgaaaatgaa
    14701 gtcctgtggt aggctcgtca aacacaaaaa tggtaggttc gggtttttcg ttggccagaa
    14761 aggaagctaa tttcacacgc tgactttcac caccggacaa agtgcttgac gactgtccga
    14821 gttttacgta tccaattccc acctcctgca atggtttaag ccgttttact attcgttttt
    14881 ctgtgtttcc gtgtccttct gaaaaaaact caatagcctg atttacagtc atattaagaa
    14941 cgtcgtagat attttttccg tgataattta cctccagaac atccgatttg aaccgttggc
    15001 cgtgacaatg ttcacattcc agcaccaaat cggccataaa ttgcatttcc acggttacag
    15061 aaccttcgcc ctggcactct tcgcaacggc cgccgggggt gttgaacgag aaaaaggaag
    15121 ctgcgaaacc catctgcctg gcgagttgct gttccgaaaa aagtttccgg atttcatcgt
    15181 aggctttgat gtaggttacc ggatttgagc gtgtgcttcg tcctatcggg ttttggtcta
    15241 caaattcaat gtctttgacc agatgcatgc ttccggataa tgatccgaat tgcccggttt
    15301 tatcggctac tcctccgtaa tattttttca gtgcagcata gaatacattt ttcaccagcg
    15361 aagacttccc cgaaccactt acacccgtca ccaccgtcat gactgacaac ggaaatttca
    15421 cgttgattcc tttcagattg ttttcgcgtg cgccgtgcac ttcaatgtaa ttattccatc
    15481 tgcgccggtg agcaggtata ttaatattct cgagttgagt taagtacttc aatgtgtacg
    15541 aattttgaat cttttcagat tgactttcca atgatatttc gtcggtattt ccctcccaca
    15601 caacatttcc accggctctt ccggcaccgg gacccatgtc gataatgtaa tcggctgcac
    15661 gcatgatttc ctcatcgtgc tccaccacca ctactgtatt tcccagatcg cgcaactgtt
    15721 tcaacacacc gataagcagg tgtgtatcgc gtgaatgtag tccgatagag ggttcatcca
    15781 gaatgtagag cgaacctacc aggctgcttc cgagtgaagt ggccagattg attcgctgac
    15841 tttcgcctcc ggaaagtgta ttggaaagcc ggttgagtgt gagataatcc aatccgacat
    15901 tcaggagaaa ttttatcctg tttttaattt cgatcagcaa tctttttgct gccagggagt
    15961 cgtgttcgtc gagtacaata ttttcaaaga aaagattcag ttgactgaca ggcagcagaa
    16021 caagttccgt gatggattta ccgtttattt tcacatagcc ggcttctttt ttcagccggg
    16081 taccgtggca ctcggggcaa attgtctttc cgcggaaacg agccagcatc accctgtact
    16141 gaattttgta ctgattagct tccagcattt tgaaaaacgc attcagtcct tcgaaatacg
    16201 aattgcctgt ccagagcagt tcacgttgct catcactcag atcaatgaat ggtttatgaa
    16261 tcggaaagtc aaatttctcg gcattatgaa tcagttctct cttccattcg ctcatcacat
    16321 cgccacgcca gcacaccaca gcgtcctggt agatggaaag tgatttgttg ggtaccacca
    16381 gatcttcgtc aattccgatg atttttccaa atccttcgca cacggggcaa gctccgagag
    16441 gattattaaa actgaacatg tgctcgttgg gcaattcaaa attgataccg tcggcttcga
    16501 aatcttttga aaaatagagc atttttgttt catcgctgtt gtacactttc agggcacata
    16561 cgccttttcc ttccagaaat gctgtttcga cagaatcagt cagacggctt acgcctgcca
    16621 ggcttcggtc tgcggtgagg cggtctatga gcagataaat atcttctttt ccttttagca
    16681 gagaagtgtt acggagcagt tcatctatcg gaaaaacctc attgccaatg gtcatccggc
    16741 tatacccgtt ttgcagaaat aaattcagtc tttcggttgt ggttatcccc ttttcaggtt
    16801 tcacgggtgc aagcaccata agtctggttt cttcgggata agtcagagca gcctgcacta
    16861 cgtcttcagt ttcgtgtttt ttaacttctt ctcccgaaac cggtgaatat gttttaccta
    16921 ttcgggcata aagaagtttg atataatcgt atatttcggt agaggtacct actgtagaac
    16981 ggggattgcg ggagtttacc ttttgctcga tagctatggc gggtggaata cctttaatat
    17041 aatccacttc gggtttggac attcgtccga ggaactgacg tgcatacgac gaaaggcttt
    17101 ccacatacct gcgctgccct tctgcataca gcgtatcaaa agccagtgaa gattttcccg
    17161 agccggatag gcctgtgatt accaccagtt tattgcgtgg aatttctaaa tctatattct
    17221 tcagattgtg aacccgcgct cccttaataa taatatttcc ctgttttggc atcctgtttt
    17281 atgtaaaaaa aaacaatttg caaaggtacg aatttaaacc agctccggca atattttcat
    17341 agttctgaaa ctatttatga tatatctaac accttatttt taaaaaatta ttcaaaaaat
    17401 ccgattagat caaaaaactt gttttttatt ttcaggcact gaattaaagt agtacttttg
    17461 cagggatttt taaaaagttt tttatacgtt aattttcaca ttcattttct taaaaatttt
    17521 ttacccatgg acacgatgac gctttactgg actattttta ttgcattatt ttttattgtc
    17581 tttttcatag atttgtatgt aaccgaccac cggaagggag aaatttcagt aaaaacttca
    17641 cttaaatgga ccggagtctg gataagcatt gctttactgt tcggtgcttc actctttttc
    17701 ttttttcctc aaaatcctga tactccggga aatacagctc atgtgatggg agtgaaattt
    17761 atttcgggat accttacgga gtattcactt tcagttgaca atttatttgt tttcatcatg
    17821 attttctcca tgatgggagt ggctgctcac aaccagccga agttgctcaa aatggggatt
    17881 attctatcca ttattttaag aatcctgttt atactggtgg ggatggaact ggtagagcgt
    17941 ttccactgga ttatttatct tttcggtttg attttagtat ggactgctta caaaatggca
    18001 tttcaggata gtgaagattc tgttgatccg aaatcaaact tcctgtataa aggtgcttcg
    18061 aaaatttatc cggtagatcc ggatactcat tccacaaaat tcttcacaaa acacaacgga
    18121 acaagataca tcaccatgaa cctgctggta ctgctggtaa ttggttctac cgatgtttta
    18181 tttgctgtag attcgattcc ggccatcatt ggcgtaatca aagaaggtgc atcgggcata
    18241 cttacacttt cagaagagaa tttcctggcc atcacttcca acgtatttgc agtaatgggt
    18301 cttatttcat tgttctttgc cttgaagggc atcatgggaa tgtttcgttt tctgaaacac
    18361 ggtgtgagct tcatcctgtt ttttatcggg gcaaaaatgc tgttgggcgc agttcctgca
    18421 gttgctcaat tctttgcaag ccattcctgg gtttcacttg cggttatcgt atttacactt
    18481 gccttctcga tacttctttc gatggtcatt gccgagaaaa aagagaacac cgagctgaaa
    18541 gccgaactgg aattggaaaa aaaggaagat gaaagagtcg acgaggttta atgccaaatc
    18601 atgatttaat tgacgccttc agtatttttt taaaaaacta aaaatgcaaa acttatccga
    18661 aaaaactgaa ttgaaacctc aaaatcctgt aattgactgg ataatcagaa tggtaaaagg
    18721 tgtgattatc ggcattggat ttatacttcc gggtctgtcg ggaggcgtat tggcggtcat
    18781 tttcggactg tacgacaaga tattgaaatt tctggccaat attaaggaag attttatcaa
    18841 aaatgttctt ttctttatac ctgttggcat tgggatggct atcggtattt tccttttttc
    18901 ggttgttgtc gaaaaagctt tcggaaagta tgctgccata ttcatttgtc tctttgtagg
    18961 atttgtcatc ggtacgtttc cctcacttta tcgcactgca ggcaaacagg gtcgtaaaac
    19021 caaagacatc cttatcatgc ttatttcggc actggttctt ttcggtctga tgatgcttgg
    19081 cgagaaacaa ttgatggatg tagcaccggg cattctttca tggcttggat cgggagctct
    19141 gatcagttta ggattgatag tacccggcat gagtccgtcg aatttcctga tttactttgg
    19201 attgtatgac aaaatggctt ccggcatcaa ggatctggat ctctcagtca tcattcctct
    19261 cattgtgggt gcagtaattt gtgtgcttct ttttgcaaaa ttagcagcac atcttttcag
    19321 gaaatattac tcgggtatgt accattttat acttggcatg gttattggct catcggtagc
    19381 catttttccg gctatcgttt ttccggcatt aaagccagaa gtgttgagtg cggcaggatt
    19441 gagtatcgcc ggagcggtac ttctttgtat tgccatgctg atagcaggca ctgttgcctc
    19501 gtatcttttc agtaaggtag aagaaaaaca tccgagagag gatatatttt agttttaaaa
    19561 acatcatttt tttcgaattc taaaaaaaac ataacgtaca tttttttaaa acaaattaaa
    19621 ttacaaatca tggaactttc acaactgttt cccgccatcg cgggaatgac ctggcaaaat
    19681 ctggtgatga ttgcagtggg gctgacgctt atctatttgg ccatcaaaaa gcaatacgaa
    19741 cctacgttac tgctgccgat gggttttggt gcaattttgg ttaatatccc atttacggct
    19801 gcactgacac agattgaccc tgctaccgga aaagctctcg aaggagcttt ggatgtattt
    19861 ttcaaggcag gtattgctac cgaagtcttt cctctgctca tctttgtagc catcggtgca
    19921 atgattgatt tttcgcctct tttccaacgg ccttcgttgt tgctattcgg cgcagctgca
    19981 caatttggta tttttgttac catggtattt gccactgcag taggatttaa cctgaaagaa
    20041 gctgcttcaa tcggcattat cggagctgcc gacggaccta cgtccatcat cgtatcatcc
    20101 attcttgcgc ctaatctgct gggccctata tcagtggcag cgtattcata catggctctg
    20161 gtaccaatta tccagcctcc ggtagtcaga ttgcttacct cacgcaaaga aagactcatg
    20221 cgcatgacct ctgattcgaa caagaaagtt tcaaaaacag ctttgattct gttcccaatc
    20281 atggttacga ttctggccgg attaattgct ccttcttcgc tggctctgat cggattcctg
    20341 atgttcggta atctgatccg tgagtgcggt gtgttgaaca gtttgtcgca aacagctcag
    20401 aaagaactgg ccaacctggt gacaattctg cttggtataa ccattgcaag caccatgacg
    20461 ggcgaacgtt ttgtaacgat agatacaatt aaaattttag gattaggaat ttttgccttt
    20521 gtatttgata ctgcgggagg tgtattgttt gccaaagtaa tgaatttatt cctgaaggat
    20581 gaaaagaaaa tcaatccgat ggtgggtgct tgcggtattt ctgcattccc gatgtcggca
    20641 cgtgttatcc acggcatggg tcagaaagaa gatccgcata acttcctgct tatgcctgcc
    20701 atcagtgcca atgtaggcgg acagattggc tccgtaattg taggtggtct tattttaatg
    20761 ttagttccat tattcgctta aaacacagaa aactatgagc ccagattttt ataaagcctt
    20821 agaaatttcc ggcattggca ttgccggcgt atttatcttc atgaccgttt tttactttgt
    20881 aatcatggga ttggataaat ttttccccaa cgagaaaaaa acaaaacaga ttgccgaagc
    20941 accgaaacag gaatacaaac cggtggaatt cagcgatgat gaatttgaat aatcagaata
    21001 cttatttctg aacaatttga caatacccgc ggttaattac tacgggtatc gttttttaaa
    21061 aaaaatcctt ctcttctgtt taaataaaac gactttgcat gttagtttgt ataggtatgc
    21121 aggctaaata aacttttttt cgtaattttg cacactcatt tctgaaaaga cagataatta
    21181 taaatgaata atatacgaaa tttttgcatc atagcgcata ttgatcacgg caagagtaca
    21241 ctagccgacc ggcttctgga gtttacccag actgtttcgg ggaaagatat gcaggctcag
    21301 gtgcttgata atatggatct ggagcgtgag cggggtatca ctatcaagag ccacgccata
    21361 cagatgaaat acgcctgtca ggatgaaata tatacgctca atctgattga caccccggga
    21421 cacgtggatt tttcgtatga agtatcgcgc tccatagctg cctgcgaagg ggcactgctc
    21481 attattgatg ctacacaggg cattcaggca cagacaattt ccaatcttta catggccata
    21541 gagcacgatc tggaaatcat accggtgatg aacaaaatgg acatggacag cgctatgccc
    21601 gaggaagtgg aagaccagat agtggatctg cttggctgta agccggaaga aataatccgt
    21661 gcgagcggta aaaccggaat ggggatagag gaaattctgg ctgccattgt agaacgtatt
    21721 ccggctccca aaggtgaacc ggatgctccg ctgcaggcgt tgatttttga ttcggtgttc
    21781 aatccgttcc gcggaattat tgcttacttc aaaattgaga acggaaccat caaaaaaggc
    21841 gatctggtga aatttgtgaa taccggtaaa gagtataatg cggatgaaat tggcgttttg
    21901 aaactggaca tgtcgcccgc cgatacgttg agtgccggca atgtaggtta catcatttcc
    21961 ggcatcaaaa cttcgaagga agtgaaagtg ggcgatacaa ttacccacgt aaaacgaccg
    22021 tgcgaaaaag ccatatccgg ttttgaggaa gtaaagccga tggttttcgc cggggtatat
    22081 cccattgaag ccgaagattt tgaggacttg agatcatcac ttgaaaaact ccagcttaac
    22141 gatgcttcac ttactttcga gccggaatcc tcggtggcac tcggctttgg ttttcgttgc
    22201 ggatttttag gtctgcttca catggagatt atccaggaac gtcttgaccg tgagtttgac
    22261 atgaatgtaa tcaccacagt accaaacgtt tcgtataagg ttcacaccaa aaaaggcgaa
    22321 ctgctcgaag tgcacaatcc ggccggactg cccgatataa tggcaattga caccatcgaa
    22381 gaaccctata tccgggcttc ggtgattacc cgaacggatt atatcggtca gattatgact
    22441 ctttgtctgg gtaaacgggg tgaactgatc aaacaggaat atgtttcggg caaccggatt
    22501 gaaatcattt acgacctgcc gctgggcgaa atcgtttttg atttctacga taaactgaaa
    22561 agcatttcca aaggctatgc ttcattcgat tatcacatga acggctggcg gccttcaaag
    22621 ctggtaaaac tggatatttt gctcaacggc gaatcggtag atgcgctctc gtcgctcatt
    22681 cactttgata atgcagtgag tctgggcaga aggatgtgcg aaaaactgag cgaactcatt
    22741 ccccgtcagc aatttgacat agcggtacag gcagccatcg gagctaaaat cattgcccgt
    22801 gaaaccatca aagccgtgcg caaagacgtg acagccaaat gttacggagg tgatgtgacc
    22861 cggaaaagaa aattgctcga aaaacagaaa aaagggaaaa aacggatgaa acaaatcggc
    22921 tcggtggaag ttccacagaa agctttcctg gcagtactga aactggatta ataaactgat
    22981 tttctaagaa aatgcttcaa ccggctattt ttttgaaaac tcaattttat attatacata
    23041 taacactcag aaaactctat tttaaattat aactaactta ttaactgcat gataacaatt
    23101 attatcatct tatttgtaat tggttatgct gccattgccc tggagcataa tataaaaatc
    23161 aacaaaacag cttcggcact gataaccggt gttgtattgt gggtagtata cattctcact
    23221 cagtctgaca ctcaccttgt aaacgaagaa cttctgaaac acctgggtga tatttcgagt
    23281 atactgtttt tcctgatggg agctatgaca atcgtcgaac tcatcgacac ttacaacgga
    23341 tttgaggtca tcaccgaacg gattactcag acaaaaaaag tgagtttact ctggataatc
    23401 ggtattttaa cattttttct ttcagccgta ctggacaacc tgacaacaac gatagtaatg
    23461 gtatcattgc ttcgcaaact gataaaagat catgaaacaa ggcttttcta tgtcggaatt
    23521 gtggtgatag ctgccaacgc cggaggtgcc tggagtccga taggcgatgt cactaccacc
    23581 atgctttgga taggggggca aatttcggca ttcaatatta tggcacgctt gtttgccccc
    23641 agtttcatca gcctggctat tccgctcgga gtactatcgc ttacgatgaa aggccgcgta
    23701 gagcgtcctt tcctggcaac agaaacatac aaggagcttt cacgaagaca acaatccatt
    23761 atcctgattt taggattgag tatgttgatt atggttccca ttttcaagac cctgacccat
    23821 ctgccaccgt ttatgggaat gatgctcgga ttgggtgttt tatggattgt gacggatatc
    23881 atgcaccgaa atcatactga agaccaaccg gaaaacggaa aacgccgcac cgtgacagat
    23941 gtgcttacac gcattgacct gccaagtatt cttttcttcc tgggtatatt actggcagta
    24001 gcttcacttg aagtgtccgg aatattacat acgctttcgc aattcctcga cgaaaagatt
    24061 ggtaatttga atttcatcgt tattgccatt ggtcttgctt cggcaatcat tgataatgtg
    24121 ccgctggtag cagctacaca ggggatgtat gatttagcgc agttcccgat ggatcattac
    24181 ctgtgggaat ttctggcata ctgtgccgga acgggaggaa gcattctcat catcggctcg
    24241 gcagccggtg tggctgccat gggcatggag aaaatcaact ttttctggta tgtaaaaaaa
    24301 atcagctggc tggcactgct cggatatttg gccggagcgg ccttctatat agttgaatac
    24361 tatttttcgc acggagcatt ttaccattaa taaaattcaa cataaatcag ggctgtcagt
    24421 aaaaatgaca gccttttttc atggttaatt ttataccttt gtatcattct gaatgaaact
    24481 atgtattcca acaaaaaaat attcaaagtt acctacccca tttttcttac gctgatagct
    24541 caaaacattg tcaacgtaac ggatactgca tttttgggac gtgtaagcga ggtagcgctg
    24601 ggtgcatcgg ccataggcgg tgtgttttac atagccattt attttgtggg atttggtttc
    24661 agtcagggtg cgcaaatcct gatagcgcga cgaaatggag agaaaaattt cggtgaaatc
    24721 gggcccattt ttaacaatgc acttatattt aactttctga ttgccacagc cattttcatt
    24781 ctctccgcta tatttatacc ggatctgata cgctttttgg ttcattcgga tcatatatat
    24841 caggcatcta ttgaatttct caactggcga atctacggtt ttttcttttc gtttctcaat
    24901 gtagttttcc gtgcattttt tgttggcatt acacgcacca aagtacttac cgtttcggct
    24961 gtcattaccg ctctcaccaa tatcgtgctt gattatctgc ttattttcgg caaattcggt
    25021 tttccggaat ggggcattgc cggcgcagct attgcatctg caatagccga attcactact
    25081 ttgatttatt ttataattgt taccctgaaa caggacgaca agcacatttt tcatttgttt
    25141 gtattcagaa aaattgattt tcataccatt ggaaaaatac tgagtttgtc cgttttcatt
    25201 atgtttcagt attttatttc tatttccacg tggtttatgt ttttcatttt tatcgaacgg
    25261 cttggcgagc ggccactggc ggtaaccaac attggcagaa gcctttacat actgctgatg
    25321 attcccggat cggcgctttc cactaccgtc aatacgctgg ttagcaatat gataggcgaa
    25381 ggtcataaag aacaggtcat tcctttcatg atgaaaatac tgaagattgt tcttgtaatt
    25441 ttgcttccgc ttatgttttt cacattcgca tttccggaac tatttgcacg tatttatacc
    25501 aataattccg atcttgtgag cgccactatt cccacactgc gggtagtttc tgttgctatg
    25561 gttttttgcg gtgtcggcag tattctgttt agtgttatct ccggcacagg aaatacaaaa
    25621 tcggcgttta ttattgaatt tctcacactg tttttctata ttgtatatgt gtattacaca
    25681 gccgtaattc tccagacttc ggtagaaatt gtctggatgt cggaatttgt gtactggact
    25741 gtaatcggac tgatggcata ctggtatctg atgaaaggta actggcaaaa gaaagaaatt
    25801 tgatgtaaaa attcattgac ccgaaccgaa aaatcaaata ccttttcatt atatttgcat
    25861 taaaatagaa tttatattcg ttatagtttg atttgtttta gtacagatct tttaccagac
    25921 actcaaaaat tccacctgct acaggtttga ggcatttcat ttacttatga aaacacacaa
    25981 atccaatatc tttcgcttcg gcgaagattt tcttaccatt ggcacagcgc tgaagcttgc
    26041 ccgcaacaat attaaaggcg ttctcactcc tgaaaccata tcccgtgtac aggccaatcg
    26101 tgatgtggta ctcaaaattg tccataagaa aaaagtggtg tacggcatca a
//
LOCUS       DAORKP010000011        25897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_57_length_25897_cov_8.462271, whole genome shotgun sequence.
ACCESSION   DAORKP010000011 DAORKP010000000
VERSION     DAORKP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25897
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57_length_25897_cov_8.462271"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLN75_01420"
     CDS             <1..231
                     /locus_tag="PLN75_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRG02805.1"
                     /translation="DIFLSLSGHTHAGQIGLTRGKIHISPASLIYPEWDGLYRHGNQY
                     LYVNRGIGYVGVPMRIGVPPEITLIVLKRRKN"
     gene            complement(276..944)
                     /locus_tag="PLN75_01425"
     CDS             complement(276..944)
                     /locus_tag="PLN75_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRG02806.1"
                     /translation="MSILQNLQEIRTHVPEHVKLVCVSKFHPENAVMEAYKAGERIFG
                     ESRVQELMEKQPNLPADIQWHFIGHLQTNKIRYVVPFVSLIHGVDSFKVLREINKQAE
                     ITGRVVHCLLQVHIADEETKFGFTTGELKEMLDSDEFSKLKNIEIDGLMGMATLTNDT
                     SKIRHEFRSLKNLKDEIKQLYFAHNESFNELSMGMSDDYKIAIEEGSTMIRIGTSIFG
                     TREY"
     gene            complement(1123..1554)
                     /locus_tag="PLN75_01430"
     CDS             complement(1123..1554)
                     /locus_tag="PLN75_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HRG02807.1"
                     /translation="MHTWFQCKVRYERNADDGSITKVNEAYLIDALSFTEAEERINEE
                     MKPYISGDFLVSDIKRAKINELFENEQGDRWYRCKVNFVSLDEEKGVEKRVATTMFAQ
                     AATLKEAVEVVEKGMKGTLADYEIASVVETNIMDVFKYKAD"
     gene            1781..2200
                     /gene="ruvX"
                     /locus_tag="PLN75_01435"
     CDS             1781..2200
                     /gene="ruvX"
                     /locus_tag="PLN75_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRG02808.1"
                     /translation="MGRILAIDFGTKRTGLAVTDPLRIMANGLETVPTKDLMPYLEKY
                     VASEQVDVVVLGLPTQMNGADSQTMQEIRAFGANFQKKFPAIELVYVDERFTSVLAHR
                     TMLEAGLKKKARQDKALVDKISATIILQSYLETLNGT"
     gene            2307..2852
                     /gene="def"
                     /locus_tag="PLN75_01440"
     CDS             2307..2852
                     /gene="def"
                     /locus_tag="PLN75_01440"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRG02809.1"
                     /translation="MILPIYTYGNAILRKVTEPITADYPNLKELVDNMYETMVHAEGV
                     GLAAPQIGLAIRLFVVDLAPLKEEHPDLGDFKVTMINPEILEESEEEVAYDEGCLSVP
                     GISETVVRPEYIKIRYFDIDFNEHILELDDFKARVVQHEYDHLEGHLFTDRIKPLRRQ
                     LLKSKLTNIAKGKAECKYKIK"
     gene            complement(2912..4069)
                     /locus_tag="PLN75_01445"
     CDS             complement(2912..4069)
                     /locus_tag="PLN75_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRG02810.1"
                     /translation="MTDQALSKKQYQYILLILILAIGFIIFQQMRPYIGGFLGASTLY
                     VLLRGQMKVLTVKKKMSRGLAATLILLEALFIFLIPLTGIGFLAVDTISGIKIDPDAL
                     KESVINVIDAIENRFNIKLFTPENMSFIPKLGTNLVQALAANSYSFVMNTVVILFVLY
                     FMLYGSDDFEATIREILPFSPENKQSFVRETKSIIQANALGIPLLAIIQGFFAYLGYL
                     IIGVSSPVLYAVLTAFATIIPLVGTAVVYIPLAIAFVIQHRYGAAIAVALYGMIVIGT
                     VDNIFRLLLQKKLADIHPLITIFGVIIGIPMFGFWGVIFGPLLLSVFILFFNMYRRDF
                     IPGSAARINITSPFKARDYKEIRNIKFPATLSKNPKKKNAKSDSASDTKSD"
     gene            complement(4196..5101)
                     /locus_tag="PLN75_01450"
     CDS             complement(4196..5101)
                     /locus_tag="PLN75_01450"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRG02811.1"
                     /translation="MEKIKVAVIGYGNIGKSVIEAIEVSPDFELAGVVRRSTVLNVKE
                     AKELNGIPVVDDIDKLGGVKVAVLCAPTRSIPDMAKKMLAKGINTVDSYDIHSSIWEL
                     KQELEPVARHYNAVSVISAGWDPGSDSVVRALLQAVAPKGITYTNFGPGMSMGHSVAV
                     KAIKGVKKGLSMTIPLGTGIHRRMVYVEIEDGVDFELVVNDIKTDAYFAHDETHVFQV
                     DDVDNLQDMGHGVNMVRKGVSGKTQNQLFEFNMKINNPALTAQVLVACARASLRLNPG
                     AYTMIEIPVVDLLYGSREDLIKKLV"
     gene            5595..8153
                     /locus_tag="PLN75_01455"
     CDS             5595..8153
                     /locus_tag="PLN75_01455"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRG02812.1"
                     /translation="MEKKIFNQQEVENETLKYFRGDDLATRVWATKYALKDSFGNIYE
                     LTPDDMHRRLASEIARIESKYPNPLSKEELFELLRDFKYIVPQGSPMTGIGNEYQVAS
                     LSNCFVIGFDGDSDSYGAIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLTVSIKHPDSESFIDAKLEAGKVTGANVSVKI
                     DDDFMKAVTEKHNYTQQYPIYSNNPWFKKDINAEALWNKIIHNAWKSAEPGVLFWDTI
                     TRESVPDCYADLGYRTVSTNPCGEIPLCPYDSCRLLAINMYSYVKNPFTPEASFDFEL
                     FEKHVQLAQRIMDDIIDLEMEKIDKILEKIQSDPENDDIKNTEFQLWEKIKAKTSQGR
                     RTGVGTTAEGDMLASLGLRYGTDEAIDFSEKVHKTVALAAYRSSINMAKERGAFTVYD
                     AKREENNPYILRLKEADEQLYNDMVKYGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVTVDFVDENGDSWEEYVVFHHKFITWMEANNISISKHYSKDEL
                     DQLVARSPYYKATSNDVDWLKKVQMQGRIQKWVDHSISVTINLPNDVSEELVGQLYEE
                     AWKSGCKGVTVYRDGSRAGVLLAVEEKKNDDEKKDDKEHCICYDSATPLVRPAVLNCE
                     VVRFQNNKDKWIAFVGLKDGRPYEIFTGIADDDDGIMLPKTVTEGKIIKVKNDEGQSR
                     YDFQFVNKRGFKTTVEGLSYKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSGLQLDSE
                     SINTWKVGVERALKKYIPDGTQISEQACPHCGQKTLVYQEGCLTCKNCAYSRCG"
     gene            8240..9190
                     /locus_tag="PLN75_01460"
     CDS             8240..9190
                     /locus_tag="PLN75_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRG02813.1"
                     /translation="MRIRAIIACLLWGSAFAGAKIGLQYAEPIFLSGVRFILAGLMLL
                     PVMYFRKINLLRAVKTHWRFMLLFAFVQTFLQYGLFFLGIDKVPGATSSIIVGAGPLF
                     VAIMAHLTLKNDKMTLRKMSAILLGFSGIVFISLAKGKISVGNPSFYPGIGLLLLSNI
                     VGSYTNIMVVKKKESNISPYALTSFANFSGGIMLLITSLIVEKPKSFQFPAEFYGALF
                     WLAFISAAAFSLWYGLLNRPGVKVSELNIWKFIIPVTGSILSWIFVKGESPDLPTILG
                     MVVIAAALLLVQAPDKYMNTVIKRTIKSINQKTQQIRHYE"
     gene            9183..9857
                     /locus_tag="PLN75_01465"
     CDS             9183..9857
                     /locus_tag="PLN75_01465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRG02814.1"
                     /translation="MNKGFDSLIFDMDGTLWDNVNSYVTAWNNGLGKTGHSERVTRAD
                     LLGQMGKEIGQMMNAIVPESTEQKRTELMYAVFDAYDELVASGNLQPTIFPDVLTGLE
                     KLKEKYKLFLLSNCEEGGLVKFMKYTGTEEIFTDYQEYGQNFLPKSKNIKLLIERNNL
                     KSTIYIGDTLSDGRETRKAGIPFGFVSYGFGNTDDYDVKFDSFGELVEYFNKLNTDNA
                     DTTDLY"
     gene            complement(9932..10897)
                     /locus_tag="PLN75_01470"
     CDS             complement(9932..10897)
                     /locus_tag="PLN75_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRG02815.1"
                     /translation="MSYVGLQTQIRRNTFRSVLMLILFPCVILMLTWLGIVAFNTFGY
                     NTHTSYPEYSVQSAETVGFLGVAPWVFGVCMIWFIIAYFSHTFIINKATGARPLERME
                     NKRVYNLVENLCMTSGMQMPIVNVIEDDSLNAFASGINKKNQTVTLSRGIINTLNDRE
                     LEAVIAHELTHIRNHDVKVMMVAIVFVGIFSFLTQFLSRVLIQRPRVSAGKNDKGSGA
                     LLIVLILVLAAIGYAITILMKLAISRKREYMADAGSAEMTKNPAGLASALRKISKDPL
                     IEAVEQNDVAQLFIENPMKKGASFLNELFATHPPIEKRIAFLEQL"
     gene            complement(10902..11459)
                     /locus_tag="PLN75_01475"
     CDS             complement(10902..11459)
                     /locus_tag="PLN75_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRG02816.1"
                     /translation="MVLTIVLIVVFLLALFVMSAYNRLVRLRNTRENAFADIDVQLKQ
                     RHDLIPQLVESVKGYMKHESDTLIKVTEARTAAMSAQSIPEKIATENQLSSALSGLKV
                     SLEAYPDLKASANFLQLQEEMADIENKLASVRRYFNTTTREYNNAVETFPANLIAGMF
                     NFRRETMFDVGDERSDLNKAPQVSF"
     gene            11701..13053
                     /locus_tag="PLN75_01480"
     CDS             11701..13053
                     /locus_tag="PLN75_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRG02817.1"
                     /translation="MANTIKINKGLDIIIEGKAIEKISKIQPSRLIEIIPDHYHGITP
                     KITVKEGEAIKAGTPLFYNKNYENMKFVSPVSGTIREIKRGERRKVLSIVIDVNVQQE
                     FLQFGIKKTSELTAGEVKSLLLESGMWTLIQQRPYDTIANPDRTPKAIFISGFDSAPL
                     APDYEFVMKEKMNDFQAGVDALGKLTSGKVHIGIKSASSIFATVKGVETTIFTGVHPV
                     GNVGVQINKVNPINKGETVWTVNPQDVAIVGRLFSKGVVEMTKTIALTGPEVENPQYF
                     ETIAGNCITDLVKGNLKKISYPLRYVSGNLLSGTQISENGFLTPYASQISVIEEGSET
                     HELFGWAMPRLDKFSASNMFFTKLLPRKKFKYDARLLGGPRSIIMSGEWDKVFPMDIL
                     PEQLIKAMIAKNIDRMENLGAYEVAPEDFALCEFVDTSKLPLQAIVREALDVLKQEVE
                     "
     gene            13218..14423
                     /locus_tag="PLN75_01485"
     CDS             13218..14423
                     /locus_tag="PLN75_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRG02818.1"
                     /translation="MNSLRKLVDKVKPNFLPGGKFEKLHSTFDAFETFLFVPNTTSKR
                     QGAHIHDAVDSKRTMIMVVIALIPALLFGMYNVGYQHFNAIGQADAGFFTLFVYGLLA
                     VLPIVVVSYGVGLSIEFAGAQIRKEEVQEGFLVTGMLIPLIVPIDTPLWMVAIATAFA
                     VIFAKEVFGGTGMNIFNVALVTRAFLFFAYPQSMSGDKVWIRTKETFGLGGGSVVDGF
                     SGATPLGQIATATTSDTTVLNMFGHPFSLMDAVTGLIPGSIGETSVIAIAIGALILLL
                     TRVASWKIMLSVFTGGALMALIFNQFGPDTAAANLPWFWHLALGGFAFGAVFMATDPV
                     TGSRTETGKWIYGFLIGALAIVIRVLNPGYAEGMMLAILLMNIFAPLIDYYVVDSNIK
                     RRLKRVANK"
     gene            14435..15157
                     /gene="nqrC"
                     /locus_tag="PLN75_01490"
     CDS             14435..15157
                     /gene="nqrC"
                     /locus_tag="PLN75_01490"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRG02819.1"
                     /translation="MNTNKNSYTIIYAAVMVVVVALLLALVSGILKEKQTANVELDKK
                     KQILSSLNISTAGQDAEALYNQYIPKAIIINYRGEVISDDRDKAFNVDIAKEMSKPLE
                     KRELPVYLATVEGQTKYILSLRGAGLWGPIWGYISFNEDKNTVFGSYFMHASETPGLG
                     AEIATKHFQNEFINKKIKNAENKFVSIAVVKPGQQAEGKDYVDGISGGTITSHGVDVM
                     LMKSIGQYENFLGQVTDGGTAK"
     gene            15154..15786
                     /locus_tag="PLN75_01495"
     CDS             15154..15786
                     /locus_tag="PLN75_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRG02820.1"
                     /translation="MSKLLSAKSKQVLLGPLSKNNPIMVQVLGICSALAVTAKLEPAL
                     VMGLSVTIIVALSNVIISLLRNTIPNRIRIIVQLVVVAALVTVVSEFLKAFAYDVSVQ
                     LSVYIGLIITNCILMGRLEAFAMGNKPWESFLDGIGNGIGYAWILAAVAFVRELLGSG
                     TLLNYRIIPQSFYDAGYVNNGMMMMPPMALILVACLIWIHRAANKDLQEK"
     gene            16009..16626
                     /gene="nqrE"
                     /locus_tag="PLN75_01500"
     CDS             16009..16626
                     /gene="nqrE"
                     /locus_tag="PLN75_01500"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRG02821.1"
                     /translation="MENIANIFVKSIFVDNMIFAYFLGMCSYLAVSRNVKTSLGLGLA
                     VIFVLGITLPVNYLLENYVLKEGALKWLSPGLASLDLSYLAFILFIAVIAAMTQLVEM
                     VVEKFTPSLHASLGIFLPLIAVNCAIMGGSLFMQQRAFVNIGEATSYALGSGVGWLLA
                     IVGLAAIREKMEYSNVPKPLRGLGITFITVALMGIAFLAFSGLKI"
     gene            16716..17969
                     /gene="nqrF"
                     /locus_tag="PLN75_01505"
     CDS             16716..17969
                     /gene="nqrF"
                     /locus_tag="PLN75_01505"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRG02822.1"
                     /translation="MNPTAVFSSLAVFLIVILLLVVILIIAKNYLVQSGEVKITINDD
                     KVITTEAGGSLLTTLAVNNIFLPSACGGGATCAECRCKVESGAGEILPTEAVHFSRKE
                     IKDNWRLGCQVKVKNDLSIKVNPSILDIKKWECEVISNHNVATFIKEFKVKLPAGEHL
                     NFVSGSYIQIDIPKYNLKYSEFDVEERFRAEWDKYKMWDLTASNADETMRAYSMANHP
                     AEGDIVMLNVRIATPPWDKAANNWAKVPAGIASSYIFNLKPGDKVFVSGPFGEFHPVL
                     DTKNEMIYIGGGAGMAPLRSHLLHLLETLKIKDRKIAYYYGARALNEVFYMEDFLKLE
                     KEFPNFTFHLALDRPDPAADAAGVKYTAGFVHQVLHDTYLSKHDAPEDIEYYMCGPGP
                     MAKAVENMLWDLGVPRENLRFDDFG"
     gene            complement(18080..18478)
                     /locus_tag="PLN75_01510"
     CDS             complement(18080..18478)
                     /locus_tag="PLN75_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02823.1"
                     /translation="MEIKIKTSLFVVLIVSLSLSKMGCESENVNDRGKLMKSWTRSYE
                     EETAGMMMFRPSDFKEFAPSRYRQVIQFENNNKCVYSVLAPNDAHFMAAGTWYFNETE
                     KNIQIRNAENEILYDYKVVILEKNKLVVTN"
     gene            complement(18635..20617)
                     /locus_tag="PLN75_01515"
     CDS             complement(18635..20617)
                     /locus_tag="PLN75_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRG02824.1"
                     /translation="MIFDLSSKITLERIPIRYYRTTDPVEAIRQTRFEKLRTEIYETE
                     TEGAAVVAREIASLIRLKAEKGQTCVLAIPGGYTPMSVYKELVRMHREEELSFRNVYI
                     FNVNEFFPVNIKQHSNGRILREYLISKVDLPEGHFFTPDESITRENVYEFCQNYEAKI
                     EELGGLDYIIASIGMVGNLAYNESGSQTGSTTRLMLLDAQSRTDLQHFYLSSADVPES
                     AITMGIATFLAARKAVLLVWGETKSSILKDIIEGKADDKVPASFLQLHKNAHAVVDLT
                     AAQHLTRISHPWLVGSCEWSDMLTRRAIVWLCQQTGKPILKLTNKDYNQYGLNELLAL
                     YGSAYNVNIKIFNDLQRTITGWPGGKPNADDSNRPERANPYPKRVIIFSPHPDDDVIS
                     MGGTFQRLVEQNHDVHVAYQTSGNIAVGDDEAIRYLALMGNVLERFDPSDNILKGKYQ
                     DMYRFLTQEKKTGDVDTIDILYLKGRIRREEARAACRYNGVPMDRVHHLDLPFYETGK
                     IKKGDISEKDVKIIVDLLQSVKPHQIFVAGDLADPHGTHKVCLDAALAAIHELKDEAW
                     MDDCRVWMYRGAWAEWEIDHIEMAVPISPEELRRKRNSILKHQSQMESAPFLGNDERL
                     FWQRAEERNQATANMYSKLGLASYEAIEAFVEYKKM"
     gene            complement(20692..21141)
                     /locus_tag="PLN75_01520"
     CDS             complement(20692..21141)
                     /locus_tag="PLN75_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRG02825.1"
                     /translation="MIYTTLSESKRIESLHPKFRELFDFVKNNDLLNRETGRIELDGD
                     LLFINNVNPTMMNSEEQVLEVHRQYIDVHFPLDRAEIIGVKPLADCTALKSSYNEKDD
                     YALYSDAPTNLVTVQPGEFLIVYPEDAHAPVIGDGKLRKLIAKVKLD"
     gene            complement(21236..22456)
                     /locus_tag="PLN75_01525"
     CDS             complement(21236..22456)
                     /locus_tag="PLN75_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HRG02826.1"
                     /translation="MKTKFLLLVTISFFLFGCKAKEKDEIPQLTWLEVKSIPESFGGI
                     SKGVSAAFTGILDGNLIVAGGCNFPGVPAAEGGKKVYYRDILMLDGSEWKKIGELPEE
                     VAYGVSVVTDDKLIFVGGQNVKPLKSVYVLSFENDTLRLDTLPSLPVGIENASGALHN
                     NRIYVAGGNVDGNPSSDVWSFDLLNSKHWTKEMPLPVEGGLVQPVLVSQLHENSSGEK
                     NLNGAKLKLSSSMQQLYLFGGFTPASGSRPAIVNQDVWEGYYGPKEGWVKSEKPFPSN
                     VVHSSLSGGAGVAIQDSLILLLGGVNRQIFEDALNRNYYLSTSKDTDTDTLTNRLRTE
                     AAEYLLHPVEWYRFNDEVWLYNVNRKEWKSIGNFPQAALAGASVASNGKEIYILNGEI
                     KPGIRTPKIWKITF"
     gene            complement(22470..24098)
                     /locus_tag="PLN75_01530"
     CDS             complement(22470..24098)
                     /locus_tag="PLN75_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRG02827.1"
                     /translation="MKKILTVAFLLVFTVSAWLNAQNTIYIYEPQVPVLIERTDNVLM
                     YIRVTSKSGSVLNSIDLQLANDVNLKNIKSLKVFYSGTEADQRKEGTFYSPVGYIAGT
                     AGAGLAANPSYSVLKSEQKNPKRNVRFTVNQSLFPGINYFWISIQMKPKASLYTTVSS
                     RITNINIDSQPAQFDYKSSSPVRRMGVGVRHGGDDGSAAYRIPGLATSNKGTLLGVYD
                     VRYNSSVDLQEHVDIGLSRSTNGGKTWEKMRIPMTFGEYNGLPSAQNGVGDPAILVNE
                     KTGTIWIVAAWTHGMGNRRAWFNSLPGMEITETAQLVLTRSDDDGKTWSAPINITPQV
                     KDPSWRFLLQGPGRGITMHDGTIVFATQFIDPQGLPHAGLMYSKDNGTNWTISTPARS
                     NTTEAQVAEVQPGILMLNMRDNRGGSRAVSITRDFGKTWEEHPSNRSALQEPVCMASL
                     LQVHAKDNVLGKDILLFSNPNTVKDRKDITIKASLDGGNTWKESNQLLLDEEYGWGYT
                     CLTMIDRETVGILYESSVAHMTFQAVKLKDIIKD"
     gene            complement(24305..>25897)
                     /locus_tag="PLN75_01535"
     CDS             complement(24305..>25897)
                     /locus_tag="PLN75_01535"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRG02828.1"
                     /translation="DAYTGGSTLELKGNATTTGTDIVLYRTDLEISAANPKVTVAVKS
                     YKEGTNPTNLFVILKKKDNDTWTEIPVGNTTDKTWQEMTLPVTGFATGDHIEYIGLRV
                     KAASAEDYQMYVGKLALTDDREVKEVAPVEDLIVEVKRETQSTMAVKLNWKISPLAAS
                     KRAEQTGLLYNDEANVDHFEIMYKNGVDGRISELARTSTWSAFVGDIIFNDTQTLEDP
                     YIGVRSASVDGKNYSPIVWIQVPRAAASQLPIFVNDGYCASEMNPDAEGAAIARVQRY
                     LTEVKTTGLTENLNYTASAPVADGTQYKNATDYSFTAAQGQTFDFFFKAYDASDGLKW
                     CFAKAYIDWDKNGDFDAVNEIVFDLGTNRAATPEFQSTGVTKSFTVPTNANTGQTRMR
                     IVFSDAWFTHPGPCGQTAKGFSIDFNVNITGTNPPVIAVDKHDQGIADEPDKVRGDGT
                     NVENLVGNPAEVSRFFPNPVETYINFRNTQQAWIYTIDGKLVFTQKGNLENVNAESLE
                     KGIYVVKMLNNGVVRSSKMQKR"
BASE COUNT         7336 a         5730 c         5482 g         7349 t
ORIGIN      
        1 gacatttttc tttcgctctc cggacacact catgcggggc aaataggcct cacacgagga
       61 aaaatacaca tttcacccgc ttcacttatt tatccggaat gggacggact ataccggcac
      121 ggcaatcagt atctgtatgt aaatcgggga ataggctatg tgggagtgcc tatgcggata
      181 ggcgtaccgc ctgaaattac gttgattgtt ctgaagagga gaaaaaatta aagaataaat
      241 aaatcaaatc aaattccgat acctggaaca tgaaattaat attctcttgt tccaaaaata
      301 gaagttccga tgcgaatcat cgtactgcct tcttcaatgg ctattttgta gtcgtcggac
      361 atacccatag acaactcatt aaacgattca ttatgagcaa aatacaactg tttgatttca
      421 tctttcagat ttttcagact cctgaactcg tgccggattt ttgacgtatc atttgtgaga
      481 gtagccattc ccatcaatcc atcaatttca atatttttca gcttactgaa ctcgtcactg
      541 tcaagcatct ccttcaattc accggtagta aagccaaatt tggtctcctc atcggctata
      601 tgcacctgca gcaggcaatg caccacccta ccggtaattt cggcctgttt gttgatttct
      661 ctgagcactt tgaaactgtc cacaccatga ataagcgaaa cgaacggcac gacgtatctg
      721 attttgttgg tctgcagatg accaataaaa tgccactgaa tatctgcagg caggttgggt
      781 tgtttttcca tcagttcctg tacacggctc tcgccgaaaa tccgctctcc tgccttgtac
      841 gcttccataa cagcattttc tggatgaaat ttggacacac agacgagctt cacatgctcc
      901 ggcacatgag tacgtatttc ctgcaggttt tgtaaaatgc tcataaactg ttttttatgt
      961 ttcgttcacc tgataattag ttggttatcg ggttattaat ccgaaaatcc ggtgccttaa
     1021 atattttttt aaaaaaacac caaactcaga actcagaact cagaactcaa tactcagaat
     1081 tttttacctg ccagaagctg cctgacttct gaattctgaa ttctaatcag ctttatactt
     1141 aaacacatcc atgatattgg tttcgacaac cgatgcaatt tcgtaatcgg cgagtgtacc
     1201 tttcatgcct ttttccacta cctctacagc ttctttaagc gtagcagcct gagcaaacat
     1261 ggtcgtagca acccgtttct caaccccttt ttcttcatcc agcgatacaa aattcacctt
     1321 acaccgatac cagcggtcac cttgctcgtt ctcaaacagc tcgttgattt tggcacgttt
     1381 gatgtccgac accagaaaat cgccgctgat gtagggtttc atctcttcgt tgattctctc
     1441 ttccgcttct gtaaacgaca aagcatcaat cagatacgct tcatttactt ttgtaatact
     1501 gccatcatcg gcgtttcttt cgtatctcac tttacactga aaccaggtat gcatactttt
     1561 aactatttat atattaatta tttacgttac gataagttga ttttcaaatt tcaaacaaag
     1621 atacggtttt tttgaaaaga aaagtgtatc tctaaccgca aaaacggtca gaaaaatatt
     1681 aacagacaga aattaagcgc aataaaagca ctaaaaaaaa ataccattta ttcatttggt
     1741 acttttttcg tacctttgca gttgataaat acaacgactg atgggtagaa ttttagcgat
     1801 tgacttcggc acgaaacgta caggtttggc tgttaccgat cctctccgga tcatggccaa
     1861 cggactggaa actgtgccga caaaagattt gatgccttat ctggaaaaat acgtggcaag
     1921 tgagcaggtg gatgtggtgg tacttggact gcccactcaa atgaacggtg ccgattcaca
     1981 aacgatgcag gaaatcagag cgtttggtgc taattttcaa aaaaaattcc ctgccatcga
     2041 acttgtatat gtcgatgagc gttttacttc ggtattggct cacagaacca tgctggaggc
     2101 aggactaaag aaaaaagcac gtcaggataa agcgttggtt gacaaaatca gtgcaacaat
     2161 aattttacaa agttatctgg aaaccctgaa cggtacataa attaatgcaa taatagtttc
     2221 tgacttctga tctgccagag gcagacaggc ttcttacttc taatttctaa tttctaattt
     2281 ctgacttcta atttctgact ttaaaaatga ttctacccat atacacctac ggaaacgcaa
     2341 tattacgaaa agtaactgaa ccgataacag ccgattatcc aaacctgaaa gaactggtag
     2401 ataacatgta cgagactatg gtccacgccg aaggtgtagg tttggccgct ccacaaatcg
     2461 gattggccat acggctgttt gtagttgatc tggcaccgct caaagaagaa catccggatt
     2521 tgggcgactt caaagttacg atgattaatc ccgaaatact cgaagaaagc gaagaagaag
     2581 tagcttatga cgaaggctgt ctgagcgtac ctggaataag cgaaacggtt gttcgtccgg
     2641 aatacatcaa gatccgctac ttcgatatcg acttcaacga gcacattctc gaactcgatg
     2701 acttcaaagc ccgggttgtg caacatgaat acgaccacct ggaaggacat ttgtttaccg
     2761 accgtatcaa gcccctccgg cgtcagttgc tcaaaagcaa gcttaccaac atcgcaaagg
     2821 gaaaagcaga atgcaaatat aaaatcaaat aatataaaaa aatccggttt atcaaccgga
     2881 ttttttttct tagaacaata ttttctgtat gctaatcgct tttagtgtca gatgctgagt
     2941 ctgatttagc attttttttc tttggatttt tcgagagtgt agccggaaat tttatattcc
     3001 ttatctcctt ataatccctg gctttgaatg gcgaggtgat gttaattctg gcagccgaac
     3061 cgggaatgaa atcgcgtctg tacatattaa aaaacaaaat aaataccgat aacagcagag
     3121 gcccgaaaat cacaccccag aatccaaaca tcggaattcc gataattacc ccaaaaatag
     3181 ttataagcgg atgaatatca gccagttttt tctgcagtag cagacgaaat atattatcaa
     3241 ccgtaccgat tacgatcatg ccatacagtg ctacagcaat ggcagctccg tatctgtgct
     3301 gtatgacaaa ggctattgcc agcggaatat acaccaccgc tgtgcccacc agcggaatga
     3361 tagtggcaaa tgcagtaagc acggcgtata aaaccggact ggacactccg ataattaaat
     3421 accccagata ggcaaaaaat ccctgaataa ttgccagcaa aggtattccc aatgcattag
     3481 cctgtataat tgattttgtt tcacgaacga aagactgctt gttttcgggc gaaaagggca
     3541 gaatttcgcg tatggtggct tcaaaatcat cggaaccgta aagcatgaaa taaagtacaa
     3601 acaatattac caccgtattc attacaaaag aatacgaatt ggctgccagc gcctgaacca
     3661 gattagtgcc cagttttgga ataaacgaca tattctcagg cgtaaacagt ttgatattaa
     3721 aacggttttc aatggcgtca ataacattaa ttaccgattc tttgagtgca tcaggatcaa
     3781 ttttaattcc cgaaatagta tcgactgcca aaaatccgat tccggtaagc ggaataagga
     3841 aaataaacag tgcttcgagc agaatcaatg ttgcagctaa tccccgcgac attttctttt
     3901 tcacggtcag cactttcatc tgtccgcgca gcaacacata cagcgtgctg gctcccaaaa
     3961 aaccgcctat gtaaggacgc atttgctgga agataataaa accaatagcc agtataagta
     4021 tcaacaatat atactggtat tgttttttgg ataatgcctg atctgtcatt tgctgtgttt
     4081 tatttttggt aactaattca tttatatatg tttaaataaa atctgcataa ttcgttttca
     4141 tacctttaat aattgacaca tgatgatttc attcctgaaa cacattttgg ttgtattaca
     4201 caagcttttt aatcaaatct tccctgctgc catacagcaa atcaactacc ggaatttcga
     4261 tcatggtgta tgctcccgga ttcaaacgca gggatgcacg ggcacacgcc acaagcacct
     4321 gtgcagtgag cgccggattg tttattttca tgttgaactc gaaaagctgg ttctgcgttt
     4381 tacccgaaac acctttgcga accatattca cgccgtgacc catatcctgc aggttatcca
     4441 cgtcatctac ctgaaatacg tgcgtttcgt cgtgagcaaa atatgcatcc gttttaatgt
     4501 cgtttaccac cagttcgaaa tcaactccgt cttcaatttc tacatatacc atacgcctgt
     4561 gaataccggt gccgagagga atggtcatcg aaagtccttt tttcactccc tttatggctt
     4621 ttacggctac agaatgcccc atgctcatac ctggtccgaa attggtatac gtaatccctt
     4681 tgggagcaac agcctggagc aacgcccgca ctaccgaatc gctgcccgga tcccagcctg
     4741 ccgaaatcac cgatacagca ttgtaatgcc tggctaccgg ctccaactcc tgcttcaact
     4801 cccagatact actgtgaata tcataactat ccaccgtatt gataccctta gccagcattt
     4861 tttttgccat atcaggtatg ctgcgcgtag gtgcacacaa tacagccact ttcacaccgc
     4921 ccagcttatc aatatcgtca acaaccggaa ttccgttcag ttcttttgct tccttaacat
     4981 tcagcaccgt acttctgcgc accacaccag ccagttcaaa atcgggtgaa acttcgatgg
     5041 cttctatcac tgatttacca atattcccat agcctattac ggcaactttt atcttttcca
     5101 ttttattttc aattttatat ttgttgcaaa attactaaaa ttttgaaagt ctgtcatgta
     5161 aaaatctatt ttcttgattg tttttcaaaa gttgaatatt ttctgtggac atgtaaaaca
     5221 aacaaattaa aagagctatc cattcatggg attaaattca atcaaattca ggaacttaag
     5281 aaaaagtaga aatatttttt ctgaaaattg atataaaaca aaacaatgca taaacttaag
     5341 aaaaaaaagt gtattaaaaa cgtaaaaaat atttttggaa aactttttgt taaaaacaaa
     5401 tacatatatc actgatatac tgttatttaa tttttatttt ggaaaatttt atataatcgc
     5461 aataaaactt tttgtaatta ttttgcctta tattcaaaat atttcactaa cattgtagtc
     5521 ttgaaaattc aattctgaat tttatataag tctctgaatt taatctgtaa acaacataaa
     5581 caacaaaaaa aattgtggaa aaaaagattt ttaatcaaca agaagtggaa aacgaaacgc
     5641 tgaaatattt tcgcggcgac gacctggcta cgagagtatg ggcaaccaag tatgcgttga
     5701 aagattcatt tggcaacatc tatgagctta caccggacga tatgcaccgc cggcttgctt
     5761 cagaaattgc ccgaattgaa tcaaaatatc caaatccact ttcgaaggaa gaacttttcg
     5821 aacttctcag ggacttcaag tacatcgtgc ctcagggcag ccctatgacc ggtatcggaa
     5881 atgaatacca ggtagcatcc ttgtccaatt gttttgtcat tggtttcgat ggcgattcgg
     5941 attcctacgg cgcaatcatc aaaattgacg aagaacaggt tcagctgatg aaaagacgcg
     6001 gcggagtagg tcacgacctg tcacacatcc gcccgaaagg atcgccggta aagaactcgg
     6061 cacttacatc caccggtatt gtacctttta tggaacgcta ctccaactca acccgcgaag
     6121 tggctcagga cgggcgccgc ggagctctca tgcttaccgt atccatcaaa catcctgatt
     6181 cggagtcgtt tatcgacgcc aaactggaag ccggaaaagt tacgggtgcc aacgtatcgg
     6241 tgaaaattga cgatgatttt atgaaagctg taaccgaaaa gcacaattac acacagcagt
     6301 atcctatcta cagcaacaat ccgtggttca aaaaagacat caatgccgaa gcactctgga
     6361 ataaaatcat tcacaatgcc tggaaatcgg ccgaaccggg cgtactcttc tgggatacga
     6421 ttacccgcga atcggtaccc gattgctacg ccgacctggg ttaccgaact gtttcgacca
     6481 atccgtgcgg cgaaattccc ctgtgtccct acgacagctg ccgcctgctg gccatcaata
     6541 tgtattcata cgtgaaaaat ccattcaccc ccgaagcatc gttcgatttc gaactgttcg
     6601 aaaaacacgt acagctggct cagcgcatta tggacgacat catcgatctc gaaatggaaa
     6661 aaattgacaa gattcttgaa aaaattcagt cggatccgga aaacgatgat atcaagaaca
     6721 ccgagtttca actctgggaa aaaattaagg caaaaacttc acaaggccgc agaacaggcg
     6781 ttggcactac tgccgaaggc gatatgctgg catcactggg acttcgctat ggaaccgacg
     6841 aagcaatcga tttctccgaa aaggtacata aaaccgttgc ccttgcagct taccgttctt
     6901 caataaatat ggcaaaagaa cgcggtgcat ttacagtata tgatgccaaa cgcgaagaaa
     6961 acaatccgta tatcctgcgt ttgaaagaag ccgatgaaca attgtacaac gatatggtaa
     7021 aatatggtcg ccgaaacatt gcctgcctca ccattgcccc taccggcact accagcatca
     7081 tgacgcaaac tacttcagga atcgaacccg tatttatgcc tgtgtacaca cgccgccgga
     7141 aagtgaatcc gaacgacaaa aacgtaacgg tcgattttgt ggatgaaaac ggcgattcct
     7201 gggaagaata cgtggttttc caccataaat tcatcacctg gatggaagcg aataatattt
     7261 cgatttcaaa acattactcc aaagatgaat tagaccagct ggtagccaga tcgccctact
     7321 acaaggcaac ctccaacgat gtggactggc tgaaaaaagt tcagatgcag ggtcggatcc
     7381 agaaatgggt ggatcactcc atcagtgtca ccatcaatct gcccaacgac gtatcggaag
     7441 aactggtagg tcaactctac gaagaagcct ggaagtccgg ctgtaaagga gttaccgtat
     7501 atcgcgacgg gtccagggca ggagtacttt tagctgtgga agaaaagaaa aacgacgacg
     7561 aaaagaaaga cgataaggaa cattgcattt gctacgattc tgccacaccg ctggtacgtc
     7621 cggcagtatt gaactgcgag gtggttcgtt tccagaacaa taaagacaaa tggatagcct
     7681 ttgtagggct caaagacgga agaccttacg agattttcac cggaatagcc gacgatgacg
     7741 acggaataat gctgcccaaa acggtgactg aaggaaaaat catcaaggta aaaaacgatg
     7801 aaggacaaag ccgctacgac ttccagtttg tgaacaaacg gggcttcaaa accaccgtgg
     7861 aaggactttc gtacaaattc gacaaggaat tctggaacta tgccaagttg atatcgggtg
     7921 tattgcgtta cggaatgccc atcgatcagg tcatcaaact ggtatcgggc ctgcagctcg
     7981 acagcgaatc catcaatacc tggaaagtag gtgtggaacg cgccctgaaa aaatacatcc
     8041 cggatggcac acaaatctcg gaacaagcct gcccgcattg cggacaaaaa acactggttt
     8101 atcaggaagg atgcctcacc tgtaaaaact gcgcttactc ccgctgcgga taggataaat
     8161 ttattttttt gataattttt ttagcaagga gaaagacggg tgacaagtgc tcgtctttct
     8221 ttataaagtc atttttttta tgcggataag agctattata gcttgtttac tatgggggtc
     8281 ggcatttgca ggtgccaaaa tcggtcttca gtatgccgaa ccgattttcc tttcgggcgt
     8341 ccggtttata ttagccggat tgatgttgct gccggtgatg tatttcagga aaattaactt
     8401 gctccgggcg gtgaaaactc actggcgatt catgcttctg tttgcattcg tacagacatt
     8461 tctgcagtac ggactgtttt ttctcggcat cgacaaagtg cccggtgcca cttcatccat
     8521 catcgtagga gccggaccgc tctttgtggc catcatggca cacctgaccc tgaaaaacga
     8581 caaaatgact ctgcgaaaaa tgtcagctat cttgctgggt ttttcaggaa ttgtatttat
     8641 cagcctggca aaaggaaaaa tatcggttgg taacccgtcg ttctacccgg gaattggctt
     8701 gcttctgctg agcaacattg taggaagtta cacaaatatt atggtggtaa agaagaaaga
     8761 gagcaatatt tcaccttatg ccctcacatc gtttgccaat ttttcgggtg gaattatgtt
     8821 gctgattacc tcactcatcg ttgagaaacc aaaatcgttt cagtttccgg ccgaatttta
     8881 cggtgcattg ttttggctgg catttatctc ggcagccgca ttctcgcttt ggtacggatt
     8941 gctcaacagg cccggtgtaa aagtatctga actgaacatt tggaaattca tcattccggt
     9001 tacaggaagt attctgagct ggatttttgt gaaaggtgaa agccccgatt tgccgaccat
     9061 tctcggcatg gtggtgattg cagctgcttt gctgctggtg caggcacctg ataagtatat
     9121 gaatactgtg ataaagagga caataaaaag tataaatcag aaaacacagc aaatcaggca
     9181 ctatgaataa aggctttgac agcttaattt tcgatatgga cggcacactg tgggacaacg
     9241 taaattccta cgtgacagca tggaacaatg gtttgggaaa aaccggacac agcgaacggg
     9301 taacgcgggc tgacttgctg ggacagatgg gaaaagaaat aggacagatg atgaacgcca
     9361 tcgtacccga aagcaccgaa caaaaacgta ccgagttgat gtatgccgtc tttgacgctt
     9421 atgacgaact ggtagcttcc ggaaatttac aaccgaccat ttttcccgac gtacttaccg
     9481 gactggaaaa gctgaaagaa aaatacaaac tttttctcct cagtaactgc gaagaaggtg
     9541 gcctggtgaa attcatgaaa tataccggaa ccgaagaaat ctttaccgat taccaggaat
     9601 acggtcagaa cttccttccg aaaagcaaaa acataaaact gctgatcgaa agaaacaacc
     9661 tgaaatcaac catttatatc ggcgacacac tcagcgacgg tcgcgaaacc cgcaaagccg
     9721 gaattccgtt tggatttgta tcatacggtt ttggaaatac ggatgattac gatgtgaaat
     9781 ttgatagttt cggtgaattg gtggaatatt tcaataagtt gaacacagat aacgcagaca
     9841 ctacagattt atattaataa gcatcattta acattctaat aataaaatat tgaatgtctg
     9901 aatgctgtca aactgaaaac gaaaaactaa tttacagctg ttccagaaac gcaatccgtt
     9961 tctctatggg cggatgggta gcaaacaatt cattcaaaaa cgaagctcct tttttcatcg
    10021 gattttcgat aaacagttga gccacatcgt tttgctctac ggcttctatc aatgggtctt
    10081 tggagatttt acgcaaggca gatgccaatc ctgctggatt tttagtcatt tcggcgctac
    10141 cggcatcagc catatattct ctttttcggg aaatggccag tttcatcagt atcgtaatgg
    10201 catatccgat agctgccaga actaaaatca gcacaatcag tagtgctccg ctgcccttat
    10261 cgtttttccc ggctgacacc ctgggtcttt gaattaatac acgcgaaagg aactgtgtca
    10321 aaaacgagaa aattccgaca aatacaatgg caaccatcat gactttcaca tcgtgattgc
    10381 ggatgtgagt aagttcatgt gctatgacag cctccagttc acgatcattc agcgtattaa
    10441 tgattccgcg tgaaagagtt acggtttgat tttttttgtt tatcccgctt gcaaaagcat
    10501 tcaacgaatc atcctcaatc acattcacta tcggcatttg cataccgctt gtcatacaga
    10561 ggttttccac caggttgtac acccgtttat tttccatccg ttccagcggt ctggctcccg
    10621 tggctttatt gattataaaa gtatgggaga aataggcgat gataaaccag atcatacaga
    10681 ctccgaaaac ccagggtgca actcccaaaa aaccaaccgt ttcggcgctt tgaacgctgt
    10741 attccgggta ggatgtgtgg gtgttgtaac cgaatgtatt gaacgcaaca atccccagcc
    10801 atgtgagcat taaaatcaca catggaaaaa gaattaacat cagaaccgaa cgaaaagtgt
    10861 ttcttctgat ttgtgtctgt aaaccaacgt aactcacctg attagaaact tacctgtgga
    10921 gctttgttca agtcggaacg ttcgtcacct acgtcaaaca tggtttcacg acgaaaatta
    10981 aacatacctg caatcaaatt ggccgggaaa gtttccacag cattattgta ttcacgggta
    11041 gtggtgttga aatagcggcg aacggatgcc agtttattct caatatcggc catttcttcc
    11101 tgaagttgca gaaaattggc gcttgctttc aaatccggat acgcctcaag cgaaaccttc
    11161 aacccactca atgcagatga aagctgattt tcagtagcta ttttttccgg aatgctttga
    11221 gccgacattg ctgctgtacg ggcttccgta actttaatca gcgtatcgct ttcgtgtttc
    11281 atataccctt ttaccgattc cactaattgc ggaatcagat catgacgctg tttcaactgc
    11341 acatcaatat ctgcaaatgc attttcacga gtgttgcgca ggcgcacaag acgattatag
    11401 gccgacatca caaacagagc cagcaaaaaa accactatta aaacaattgt cagtaccata
    11461 ctatttcagt attaaatgtt attgttacaa agttacatta ttttttaaaa taacattcgt
    11521 gataaaacaa ataatcattt tcaaatataa ctttttagag ttaaaaaata aagttttaga
    11581 actattctaa ataaattttg tttttatagc tcaaattact gaattatttc tttattaatt
    11641 gaaaattaaa aggtatattt gcaccgtttt tttgtgaaat ttaactcaac tactgtttta
    11701 atggcaaata caataaaaat aaacaaagga ctggatatta taatcgaagg caaagccatt
    11761 gagaaaatat caaaaatcca accttcacgc ctaatcgaaa ttattcctga ccattatcac
    11821 ggcattacac ccaaaattac tgtaaaagaa ggagaggcta tcaaagccgg cactccgctt
    11881 ttttacaata agaattacga aaacatgaaa ttcgtatcgc ccgtgagtgg taccatacgt
    11941 gaaattaagc gtggcgaaag acgtaaagtg ttgagcatag tgatcgatgt gaatgttcag
    12001 caagaatttt tgcagttcgg aataaaaaag accagcgaac ttactgccgg agaggttaaa
    12061 tcactcttgc tggagtcggg catgtggaca ctgatccagc aacgacccta cgacacgatt
    12121 gccaatccgg accgtacacc aaaagccatt tttatttccg gatttgattc cgcaccgctt
    12181 gcacccgatt atgagtttgt gatgaaggaa aaaatgaatg attttcaggc cggtgtagat
    12241 gctttaggta aacttacttc aggtaaagtt catatcggga taaaatcggc cagttccatt
    12301 ttcgcaacgg taaaaggagt tgaaacaacc atcttcacgg gcgtacatcc ggtaggaaat
    12361 gtaggtgtgc aaatcaacaa agtaaacccg ataaacaagg gcgaaaccgt ctggacggtc
    12421 aatccgcagg atgttgccat tgttgggcgg ctgttctcga aaggcgtggt agaaatgacc
    12481 aaaaccattg cactgaccgg acccgaggtt gaaaatccgc aatactttga aaccatcgcc
    12541 ggaaactgca taacggacct ggtaaaagga aacctgaaaa aaatcagcta tccgctgcga
    12601 tatgtgagcg gtaacctgct tagcggtacc caaataagcg aaaacggatt tcttacccct
    12661 tacgcatcac aaataagcgt gattgaagaa ggaagtgaaa cacacgaact ctttggatgg
    12721 gctatgccgc gactggataa attcagtgca agtaacatgt ttttcaccaa actgcttccg
    12781 cgtaaaaagt tcaaatacga tgcccgtttg ctgggcggac cacgctctat catcatgtcg
    12841 ggcgagtggg acaaagtatt tccgatggac atacttcccg aacagcttat caaagctatg
    12901 attgccaaaa acattgaccg catggaaaat ctgggtgcct atgaagtggc tcccgaagat
    12961 tttgcactgt gcgaatttgt tgatacttcc aaattaccac ttcaggccat cgttcgcgaa
    13021 gcgcttgatg tattgaaaca ggaagtagag taatgacccc tgaccccgtt cggctcagca
    13081 cacgttgaag cctgaagggg tattttatgt gggataaaga gattaaataa tcatattacg
    13141 aaatatagaa atcgattaac ttttttataa acctcaagat attcccccaa tagggggtta
    13201 gggggcagat taaaattatg aattcattaa gaaaattagt tgacaaagta aaaccaaact
    13261 tcttgcccgg cggaaaattt gagaaattac actctacttt cgacgcgttt gaaacatttt
    13321 tatttgtccc gaataccaca tcaaaacgcc agggagccca tattcacgac gccgttgaca
    13381 gtaaacgtac catgattatg gtggtcattg cactgattcc ggctctgctt ttcggcatgt
    13441 ataatgttgg ctaccagcat ttcaatgcca taggccaggc cgacgccgga tttttcaccc
    13501 tgttcgttta cggacttttg gcagtacttc ccatcgttgt ggtatcgtat ggtgtaggat
    13561 tgagtatcga atttgccggg gcgcaaatcc gcaaggaaga agttcaggaa ggttttctgg
    13621 ttacggggat gcttatcccg ctcatcgttc cgatagatac acctctctgg atggttgcca
    13681 tcgccacggc ttttgcagtg atttttgcca aagaagtatt tggtggtaca ggcatgaaca
    13741 tcttcaacgt ggcattagtt acacgcgcat tccttttctt tgcctatccg caatccatgt
    13801 cgggcgacaa ggtttggatt cgaactaaag aaacgttcgg tttaggcggc ggttctgttg
    13861 ttgatggttt ttccggagct actcctctcg gacaaatagc tacagccacc acttccgata
    13921 ctactgtact caatatgttc ggtcatcctt tctcgctgat ggatgctgta acaggcctga
    13981 ttccgggctc aatcggtgaa acttctgtta ttgccattgc cattggcgct ctgatattgt
    14041 tgcttacacg cgttgcaagc tggaaaataa tgctttctgt tttcacagga ggagctttga
    14101 tggctctgat tttcaatcag ttcgggcctg acactgcagc tgccaacctg ccgtggttct
    14161 ggcatctggc tttaggaggt tttgcatttg gagccgtatt tatggctacc gatccggtaa
    14221 caggatcgcg caccgaaacc ggaaaatgga tttacggttt tctgattggc gctcttgcca
    14281 ttgttatccg tgtactcaac ccgggttacg ccgagggtat gatgctcgcc attctgctaa
    14341 tgaacatttt tgcacctctg attgactact atgtggttga ttctaatatc aaacgccgtc
    14401 tgaaaagagt tgccaacaag taaacacgat aaaaatgaat acaaataaaa atagttatac
    14461 aattatctat gctgccgtca tggtagtagt tgtagccttg ctgttggcat tagtatccgg
    14521 cattttgaaa gagaaacaaa cggctaacgt ggaacttgac aagaaaaagc agatactcag
    14581 ttcgctgaat atttccactg ccggacagga tgccgaagcg ctttacaatc agtacatccc
    14641 gaaagcaatc atcatcaact accggggtga agttatttct gacgacagag ataaagcgtt
    14701 caacgttgat attgccaaag aaatgagcaa accgctcgaa aaaagagaac ttcctgtata
    14761 tcttgccacg gtggaaggac agacaaaata catactctca ctgcgcggag ccggactttg
    14821 gggccctatc tggggataca tttccttcaa cgaagataaa aacacggttt tcggatccta
    14881 cttcatgcac gcaagcgaaa ctccgggact gggcgctgaa attgccacca aacattttca
    14941 gaatgagttt ataaataaaa aaatcaaaaa cgccgaaaat aaatttgttt ccattgccgt
    15001 ggttaaaccg ggtcagcagg ccgaaggaaa agactacgtg gacggcattt caggaggaac
    15061 cattaccagc catggcgtag atgtgatgct gatgaaaagc attggacagt acgaaaattt
    15121 tttaggacaa gtaactgatg gaggaactgc aaaatgagta agttattatc tgcaaaatca
    15181 aaacaagtat tgttgggacc gttgagcaaa aacaacccca tcatggtaca ggtactgggt
    15241 atctgttcgg ccctggccgt aactgccaaa ctggaaccgg cattggtgat gggactttct
    15301 gtaacaatca tcgtggctct ctcgaacgta attatttcgt tattacgtaa taccatccca
    15361 aaccgaatcc gaatcattgt tcagctggta gtagtggctg cgctggttac ggtggtgagc
    15421 gaattcctta aagcttttgc ctacgatgtg agcgttcagc tttcggttta tatcggtttg
    15481 attatcacca actgtattct gatgggtcgt ctggaggcat ttgccatggg taacaagccc
    15541 tgggaatcat tcctggatgg aataggcaac gggattggtt atgcctggat tctggctgca
    15601 gtggcattcg tacgcgaatt gctgggttcg ggcaccctgc tgaactacag aatcattcca
    15661 cagtcattct atgatgccgg atatgtgaac aacggcatga tgatgatgcc gccgatggct
    15721 ctgattcttg tagcttgtct tatctggata caccgggctg ctaacaagga tttacaggaa
    15781 aaataaataa ctacacctgc tctcttaccc caaaagggga gttttggtgg aattctataa
    15841 atgcagtctg aaaactgaag tttgattttt caacaattca ttaagttgaa tttaatttat
    15901 ataaatcata ttataatcat taaaaaaact aatcaacgct cttaaattcc ccattcaggc
    15961 tcctatataa gtgtagccga acgggcggct cagggggcaa ttaataatat ggaaaatata
    16021 gctaacatat tcgtaaagtc aatttttgtt gacaatatga ttttcgctta ctttttggga
    16081 atgtgctctt acctggccgt ttccagaaat gtaaaaacct cgttgggttt gggcttggca
    16141 gtaatctttg tgctgggtat tacgctgcct gtgaactacc tgctcgaaaa ctacgtgctg
    16201 aaagaaggtg cgttgaaatg gctgagtccg ggtctggctt cgctggattt gagttacctt
    16261 gcatttattt tgttcatagc agtaattgca gccatgacac agctggtaga gatggtggtt
    16321 gagaaattca ctccctcgct ccacgcatca ctcgggatat tcctgccgct tattgctgtt
    16381 aactgtgcca tcatgggtgg atcgctcttc atgcagcaac gtgcctttgt caatattggc
    16441 gaagctactt cctatgcttt gggttcgggt gtgggctggc tgctggctat cgtcggactg
    16501 gctgccatcc gcgaaaagat ggagtattcc aacgtgccga aacctctgcg cggactgggc
    16561 atcacgttta ttacagtggc gttgatgggc attgcctttc tggcattctc aggattgaaa
    16621 atatagttaa tcagtatatt gagttaattc gtgtattcgt tttctcattt ccaaatcaaa
    16681 ctaaaacatt ttatacaaga acaatgatat tagctattaa tcccactgcg gttttttcca
    16741 gtttggcggt ttttttaatc gtaatactgc ttttggtcgt aattctcatc attgccaaga
    16801 attacctggt acagtccggt gaagtaaaaa tcactatcaa cgacgacaaa gtaattacca
    16861 ccgaggcagg tggaagttta cttaccacac tggctgtcaa caacatattt ctgccttcgg
    16921 catgcggtgg cggtgcaacc tgtgccgagt gtcgctgtaa ggtagaatca ggagccggcg
    16981 agatattgcc tactgaagct gtccatttct cacgcaagga aataaaggac aactggcggc
    17041 ttggttgtca ggtgaaggta aaaaacgacc tttctatcaa agtcaatccg tctatcctgg
    17101 atataaagaa atgggaatgc gaagtgatat caaaccataa tgtggcaaca tttatcaagg
    17161 aatttaaggt aaaactgcct gcaggcgaac atttgaactt cgtttcggga agttacattc
    17221 aaatagatat tccgaaatac aacctaaaat acagcgaatt cgacgttgag gaacgttttc
    17281 gagccgagtg ggataagtat aagatgtggg atttgaccgc atccaatgct gacgaaacca
    17341 tgcgcgccta ctccatggcc aaccaccctg ccgaaggaga tatcgtgatg ctaaacgtac
    17401 gtatagccac tccaccctgg gacaaggcag ccaacaactg ggctaaggta cctgccggta
    17461 ttgcttcatc ctacatattt aatctgaaac cgggcgacaa agtctttgtt tccggtcctt
    17521 tcggcgaatt ccatccggta ctcgacacca aaaacgagat gatttacatc ggtggtggag
    17581 ctggtatggc acctctccgt tctcatttgc ttcatctgct tgaaacgctg aaaattaaag
    17641 accggaaaat agcctattac tacggcgccc gtgccctgaa cgaagttttc tacatggaag
    17701 attttctgaa actcgaaaaa gaatttccga atttcacttt ccacctggcg cttgaccgcc
    17761 ccgatccggc tgccgatgcc gccggtgtaa aatataccgc aggatttgtt catcaggtgc
    17821 ttcatgatac ctatctgagc aaacacgatg ccccggaaga tatcgaatac tacatgtgcg
    17881 gacccggtcc gatggctaaa gcagtggaga atatgctttg ggatctggga gtgccgagag
    17941 aaaatctgcg attcgacgat ttcggataat tccggccaaa acgtacagga atacaacgaa
    18001 tcaaaaaggc tccgttctca cgaacagagc ctttttactt aaactaaaaa caaacttata
    18061 aaacaaaatt tatggggaat caattagtaa caactaactt atttttttct aaaataacaa
    18121 ctttataatc atacagaatt tcattttcgg cgtttctgat ttgtatattt ttttcggttt
    18181 cattaaaata ccacgtccct gccgccatga aatgcgcatc gttaggagct aagacagaat
    18241 aaacgcattt gttattattc tcgaattgaa taacctgtct gtaccggctc ggagcaaatt
    18301 ccttaaaatc agaaggacga aacatcatca ttccagcagt ttcttcttcg taagatcgag
    18361 tccaactctt catcagctta cccctgtcat tcacattttc gctttcgcaa cccattttac
    18421 ttaatgacaa tgaaacaatc agcacaacaa acaatgaagt tttaatctta atttccatgg
    18481 attaaatttt gccttttatt atatgatgga attttacctc aaaacgctgc atcgtatgtg
    18541 ttaaataaaa ttaaaacaaa gcaaaaaaaa cagaaaacgc tcaaatctgt ttgaaatgag
    18601 cgttctctga atcattatct gataaagccg gattttacat tttcttatat tccacaaatg
    18661 cctcaatagc ttcgtaagaa gccagtccga gtttgctgta catgttggct gtagcctggt
    18721 tgcgttcttc ggcacgctgc cagaagagcc gttcgtcgtt gccgaggaat ggagcgcttt
    18781 ccatctggga ttgatgtttc agaatggaat tacgtttgcg gcgcaactct tccggactga
    18841 tcggaacagc catttcgatg tgatcaatct cccactctgc ccaggcgccc cggtacatcc
    18901 acacgcggca atcgtccatc catgcttcat ctttcagttc gtgaatggct gccagcgcag
    18961 catccagaca tactttgtgc gttccgtgcg ggtcggccag gtcgccggct acgaagatct
    19021 ggtgaggttt gaccgattgc agcaggtcaa caattatttt gacatccttt tcagaaatat
    19081 cccctttttt tattttaccg gtttcataaa acggcagatc gaggtgatgt acacgatcca
    19141 tcgggactcc gttgtagcgg caggctgcac gggcttcttc gcggcgtatt ctccctttca
    19201 gatacagtat gtcgatggtg tcaacatcgc ctgttttctt ttcctgagtg aggaaacggt
    19261 acatatcctg atattttcct ttcaaaatgt tatcggaggg atcaaaacgt tcgagtacat
    19321 tgcccatcag cgccagataa cgaatggctt catcgtcgcc tacggcaatg ttgcctgatg
    19381 tctgatacgc tacgtgtacg tcgtgattct gctcaaccag acgctggaaa gtgcctccca
    19441 tggaaatcac atcgtcatcg ggatgcggac tgaaaatgat cacccgtttg ggataagggt
    19501 tggcgcgctc cggccggttg ctgtcatcgg cattgggttt tccgccgggc cagccggtaa
    19561 tggtacgctg caggtcgttg aaaattttga tgttgacatt ataagccgaa ccatacaagg
    19621 cgagcaattc gttgagtccg tattggttgt agtctttatt ggtcagttta agaattggtt
    19681 ttccggtttg ctgacagagc cacacgatag cgcggcgggt gagcatgtcg ctccattcgc
    19741 acgagcccac cagccaggga tgactgatac gggtgagatg ttgtgcggca gtgagatcga
    19801 ctacagcatg cgcatttttg tgcaattgca ggaacgaagc cggcacttta tcatcggctt
    19861 tgccctcgat gatgtctttc agaatgcttg attttgtttc accccatacg agcagtactg
    19921 ccttacgtgc tgccaggaat gtggcaatac ccatcgtaat ggcactttcg ggtacatccg
    19981 ccgaggagag gtagaaatgc tgcagatcag tacggctttg ggcatcgagc agcatcaggc
    20041 gcgtagtgga gcctgtttgc gatccggatt cgttgtaagc caggtttcct accatgccaa
    20101 tggaagcgat gatatagtct agcccgccca gttcttcaat tttggcttcg taattctgac
    20161 agaattcata cacattttcg cgtgtaatcg attcgtcggg tgtaaagaaa tggccttcag
    20221 gcaaatctac cttactgatc aggtattcac gcaggattct tccgttggaa tgctgcttga
    20281 tgttaaccgg gaaaaactcg ttgacattaa aaatgtacac attacgaaac gaaagttctt
    20341 cttcgcggtg cattcttacg agttccttgt acacactcat cggcgtgtag cctccgggga
    20401 tggccagtac gcaggtctgc cctttttcag ccttcaacct gataagcgaa gctatttccc
    20461 gggctactac tgccgcgcct tctgtttcgg tttcataaat ttctgtccgg agtttttcga
    20521 accgggtctg acggatggct tcaaccggat cggttgtgcg gtagtagcgg atcggaatcc
    20581 gttcaagcgt gatttttgaa cttaaatcga aaatcataat ttgttttctg ttttattatt
    20641 aatttgtttg tgatttcttt cattgacatt acatgctgaa aggcaaaata cctaatccag
    20701 tttcaccttt gctatcaatt tccttaattt tccatctcca atgaccggcg catgtgcatc
    20761 ttcgggatag acgataagaa actctccggg ttgtaccgtc acaaggttgg ttggagcgtc
    20821 agaatataag gcataatcgt ctttttcgtt atacgacgat ttcagggcag tacagtcggc
    20881 aagtggtttt acaccgataa tttcagctct gtcgagcggg aagtgaacgt caatgtattg
    20941 tctgtgaacc tccagtacct gttcttcgga gttcatcatg gtcggattca cgttgtttat
    21001 aaaaagtaaa tctccgtcga gttctattct tccggtttca cgattcaaca agtcgttgtt
    21061 tttaacaaaa tcgaataatt ccctgaattt agggtgcaaa ctttcgatgc gtttgctttc
    21121 ggataaggtg gtataaatca taggatagta ttattagaat aaagttttaa caagcaattt
    21181 acgtatattg tttataaaaa aaatgcattt cttcatcaat tattcgtgat aattttcaaa
    21241 aggtgatttt ccatattttc ggagtacgta ttcccggttt tatttcgccg tttaaaatgt
    21301 atatttcttt tccgttgctt gctactgatg cacctgccaa tgctgcctga gggaaattgc
    21361 caatcgattt ccattctttt cggttcacat tgtagagcca gacttcgtca ttaaacctgt
    21421 accattcgac gggatgaagc aagtattcag cggcttcggt gcgcagtcgg tttgtcaagg
    21481 tgtcggtatc ggtgtctttt gatgttgaca aataataatt ccggttaagg gcatcttcaa
    21541 aaatctgcct gttcacacca cccaacagca atatcaggct atcctgtatg gcgactcctg
    21601 ctccaccgct taaggaacta tgtacaacat tggatgggaa tggtttttca gattttaccc
    21661 atccttcctt tggtccgtaa taaccttccc aaacatcctg gtttacaata gcaggacgac
    21721 ttcccgaagc cggagtaaat cctccgaaca ggtacaattg ttgcattgaa gaagacagtt
    21781 tgagttttgc tccgttcagg tttttttcgc cggaagaatt ttcatgcaac tgagaaacaa
    21841 gaaccggctg caccaatcca ccttctacag gcaacggcat ctccttcgtc cagtgttttg
    21901 aatttaacaa atcgaaactc cagacatccg atgagggatt tccatccaca ttgcctcctg
    21961 ccacataaat cctgttgttg tgcaaagcac cggaagcatt ttcgattccc acgggtaacg
    22021 atggcaatgt gtccagacgt aatgtatcgt tttcaaaact caacacataa accgatttca
    22081 ggggcttcac attttgtccg ccgacaaaaa tcaatttatc atccgtcaca acagatacac
    22141 cgtaagcaac ttcttcgggc agctccccga tttttttcca ttcacttcca tcgagcatca
    22201 gaatatctct gtaatacact ttcttaccgc cttcggcagc cggcacaccg gggaaattgc
    22261 agccaccggc tactatcaga tttccatcca ggatacctgt aaaagcggcg gaaactcctt
    22321 ttgaaatgcc accgaatgat tcgggaatgc ttttcacttc caaccatgtc agttgcggga
    22381 tttcgtcttt ttcttttgct ttacagccaa acaaaaagaa agaaatagtc accagcagca
    22441 aaaacttagt tttcattttt tttcaattat caatccttga ttatatcttt gagtttcaca
    22501 gcctgaaaag tcatgtgagc cacactgctt tcgtagagta ttcccacagt ttcgcggtca
    22561 atcatggtga ggcaggtata gccccatcca tattcctcgt cgagcaggag ctgattggac
    22621 tctttccagg tgttccctcc gtcaagactt gctttgatgg tgatgtcctt tctgtctttt
    22681 accgtattcg gattggaaaa aagcaatata tcttttccca ggacattgtc tttggcatgt
    22741 acctgaagca gacttgccat acacaccggt tcctgcagtg cgctgcggtt cgaaggatgt
    22801 tcttcccagg ttttcccgaa atcgcgggta atgcttaccg cacggcttcc acccctgttg
    22861 tcgcgcatgt tcaacatcag tattccgggc tgtacctctg ccacctgagc ttcggtggta
    22921 ttgctgcgtg ccggtgtgct tattgtccag ttggtaccgt tatctttgct atacatcaat
    22981 ccggcgtggg gcaatccctg aggatcaatg aattgagtag caaaaacgat tgttccgtcg
    23041 tgcatggtga ttccgcgtcc gggtccctgc agcagaaatc tccacgaagg gtcttttacc
    23101 tgaggagtaa tgttgatggg ggccgaccag gttttaccat catcgtcgct tcgggtcagc
    23161 acaagttgtg cggtttcggt gatttccatt cccggaagtg agttgaacca cgcccgacgg
    23221 tttcccatgc cgtgagtcca ggctgcaaca atccagatgg tgccggtttt ctcgtttacc
    23281 agaatggcag gatctcccac cccgttctga gccgacggaa gcccgttgta ttcgccgaaa
    23341 gtcatcggaa tgcgcatttt ttcccaggtt tttccaccgt ttgtacttct gctgagtccg
    23401 atatctacgt gctcctgcag atcgacactg gaattgtaac gcacatcgta aacaccaagc
    23461 aaagtgccct tgttggaagt ggcaagtccc gggatgcggt atgcggctga accatcgtcg
    23521 cctccgtgac gcacacccac gcccattctg cgaacgggag aggacgattt gtagtcgaac
    23581 tgagcaggct gactgtcgat gttaatgttg gtaattctgg atgaaacggt agtatacaag
    23641 gatgcttttg gtttcatttg tatgcttatc cagaaatagt tgatacccgg aaacaacgat
    23701 tgattaacgg taaagcgaac attacgtttc ggatttttct gttctgattt taagacagaa
    23761 taggacggat tggctgcaag acctgcaccg gccgtacctg caatgtatcc taccggcgaa
    23821 tagaatgtgc cttcttttcg ctgatcggct tctgtaccgg agtagaaaac tttcagcgac
    23881 ttaatatttt tgaggtttac atcatttgcc agctgtaaat cgatggaatt aagtacgctt
    23941 ccgcttttgg atgttacgcg tatgtacatg agcacattat ccgtgcgttc aattaaaacc
    24001 ggaacctgcg gttcgtaaat gtaaatcgta ttttgtgcat tgagccatgc tgagactgta
    24061 aacacaagca aaaaagcaac agtgaggatt tttttcataa attttccagt ttacacgtta
    24121 atatttcaga taattacggc aaagttaatt aaatttcttt attaatcaaa ttaaagtaac
    24181 taaatgtttt aattaattat atgataccaa aaaagtgagc aggaattgct tcctgctcac
    24241 ttttatttca gatgatctat aagccctttc ctgctatcta tctgacagat aatcaaaaaa
    24301 tctgttatct tttctgcatc tttgaactgc gtacaactcc gttgttcaac attttcacca
    24361 cgtagatacc cttttcaagc gattcagcat ttacattttc caggtttcct ttttgtgtaa
    24421 ataccagttt accatcgatg gtataaatcc aggcttgttg tgtattacgg aaattgatgt
    24481 atgtttcaac cggattcggg aagaatcggg atacttctgc cggatttccg accagatttt
    24541 caacattggt accatcaccg cgaaccttat ccggttcatc agctatgccc tgatcgtgtt
    24601 tgtcaacagc aattacagga ggatttgtac ctgtgatatt tacattgaaa tcaatgctga
    24661 atcctttggc agtctgtccg catggacccg ggtgtgtaaa ccatgcatcg gagaaaacga
    24721 tgcgcatgcg ggtttgtccg gtatttgcat tggtcggtac agtaaaagat ttggtaactc
    24781 cggttgattg aaattcggga gttgctgccc tattggtacc taaatcaaat acaatttcgt
    24841 tcacggcgtc gaagtctccg tttttatccc agtcgatata tgcctttgca aagcaccatt
    24901 tcaaaccatc actggcgtca tacgctttga agaagaaatc aaatgtctga ccctgagcag
    24961 cagtaaatga ataatctgtt gcattcttgt attgagtacc atcggctacc ggagcactgg
    25021 cagtataatt gaggttctcg gtcagtcctg tcgttttcac ttctgtcaga taacgctgta
    25081 cacgtgcaat ggctgctcct tcggcatcag gattcatttc tgaagcacaa tatccatcgt
    25141 tgacgaaaat aggaagttgt gacgctgcgg cacgtggaac ctgaatccag actatcggag
    25201 agtagttttt accatcgact gatgcagagc gaactccgat gtatggatct tctaacgttt
    25261 gcgtatcgtt aaatatgata tcacctacaa aagccgacca ggtagaagta cgtgcaagtt
    25321 cagatattct gccatctaca ccattcttat acataatttc aaaatgatca acgttagctt
    25381 cgtcgttgta aagcaagcca gtttgttcgg cccgctttga agcggcaaga gggctgattt
    25441 tccagttgag ttttactgcc attgtgcttt gagtttctct tttcacttct actatcagat
    25501 cttcaacagg tgcaacttct ttcacttccc tgtcatcagt gagggcgagt ttacctacgt
    25561 acatctgata gtcttctgct gaagcagctt tcacgcgtaa tccgatgtat tcgatgtggt
    25621 caccggtagc aaatccggtc accggcaaag tcatttcctg ccaggtttta tcggtggtat
    25681 tgccaaccgg tatttctgtc caggtatcat tgtctttctt tttcagaatt acaaataaat
    25741 tggtaggatt agtaccttct ttgtatgatt tcacagctac tgtgactttt ggattggcag
    25801 cacttatttc cagatcggtg cggtaaagca cgatgtctgt tccggtagtt gttgcattac
    25861 ctttcagttc gagggtagaa ccacccgtat aagcatc
//
LOCUS       DAORKP010000012        23020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_78_length_23020_cov_9.109645, whole genome shotgun sequence.
ACCESSION   DAORKP010000012 DAORKP010000000
VERSION     DAORKP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23020
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_23020_cov_9.109645"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..559
                     /locus_tag="PLN75_01540"
     CDS             74..559
                     /locus_tag="PLN75_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRG02829.1"
                     /translation="MTYDEYEKIKKLEELLKNGTITQEEFDKEREKIISSAPKAAPSE
                     KPFAASTRQPLMGIEENLYLLLMHLSQFLTAFIIPLVMWLVGKDENKRVDFHGKNILN
                     FQITYIIWLIVGIITIPLIIGIVILSVLGIAMTVFIIVAAVKSYNGEDWKYPLTINFL
                     K"
     gene            641..1285
                     /locus_tag="PLN75_01545"
     CDS             641..1285
                     /locus_tag="PLN75_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRG02830.1"
                     /translation="MIFTDIHTHHSLSEPDILTVRNIRMEEAESVLGTAQGGFFSVGI
                     HPWYLHNISAEDIRLLELICSDKRVVLIGECGIDKNSTFSAEMQIPVFEKQILISERI
                     SKPLIVHCVGSFNELFVLRKKHQPTQRWIIHGFRGKPQLAEQILQSGCDISFGEKFNP
                     ESVRIVPFERLFIETDQSEISIEEIYHQIAAAKNCKIHELLAGNLFINEFSSIL"
     gene            1402..2997
                     /locus_tag="PLN75_01550"
     CDS             1402..2997
                     /locus_tag="PLN75_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG02831.1"
                     /translation="MKKYISLAVFLLAVFSAHPQGISSIQQRKLAQALSAISGMYVDT
                     INDKKIIESTIRGVLEELDPHSTYIPKEEVQRMHEPLEGSFDGIGVQFQIIKDTINVV
                     QTVSGTPAEKVGVLPGDKIVYIENESVAGIKIQNTDVFKRLRGKRGTPVNIRVKRGNA
                     AELIDFKIIRDKIPIYSVDASYMLTDKIGYIKINSFGSTTTEEFNKALKTLKSKGMKD
                     LVISLERNGGGFLKTSIELADELLPAKQLIVYTEGLNAPRTEAKATAAGNFESGRVVI
                     LIDEFSASASEILSGAVQDWDRGVLIGRRTFGKGLVQREVSLADGSMMRLTVARYYTP
                     TGRSIQKPYVNGGAKDYNKDLEERFEKGEMMHPDSIHFPDSLKFNTLTANRTVYGGGG
                     IMPDIFIPMDTTENTLYHRRIVGKGVVNSTMIEYLNINRDELRKKYPSFDAFNKNYEA
                     DESLLKILIDNAEKEGIKPDEKDFNRSKRVIKLQLKAMIANDLWGLNEYYEVINSDNK
                     SLQKAVEILTTKGAYDKMLGKKK"
     gene            3095..3685
                     /gene="pnuC"
                     /locus_tag="PLN75_01555"
     CDS             3095..3685
                     /gene="pnuC"
                     /locus_tag="PLN75_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRG02832.1"
                     /translation="MKDYITENWVEIAGAVLSLLYLYLSIREKSALWIFGFISSLLYI
                     FVFFESKLYADMSLNFYYLGVSIYGWINWHRKTGQFKDSGLHITRISGKQQILSYLFG
                     TIFVYLAYYLVLELLTDSTIPVADSVVGALSVIATWMLARKKIENWLVWIVVDAFAAG
                     LYLYKGLYPTTILFIIYTIMAVVGYYQWKKTPDAIK"
     gene            complement(3678..4880)
                     /locus_tag="PLN75_01560"
     CDS             complement(3678..4880)
                     /locus_tag="PLN75_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRG02833.1"
                     /translation="MKRIAYILLLLVLISQPAFCRQKKVASFPFRLVGSTIVVETSIN
                     GSSPLNFILDTGVKNTMITEILPEDSVELPRGEIVGIRGLGLNDSIRAYHSTKNMLKI
                     GNLRLQNQDILVLMDKIISFSSITGEKINGILGSEILKDYVVEISYSRQRITFYTHES
                     YAVPGSYKRIPAIVAGSKMYVNANVINNGITNENLAMLIDTGAEIGAWFQTIRADAFE
                     LPEKRIHGYIGEGLNGEIHGYYAVIDSLNIGPFYLKKPIVTFPDSLYIADFIIRSSRD
                     GTLGNQVLKRFDSFIDFKTPALYLKPNRMFAEKFSYNISGIEMTQNFPYIFDITVHKV
                     WKNSTADLKGIKPGDLILEVDKQPVFTMKMVEIRKILETPRKRPLHIVIQRDLEVLDV
                     YLDMKSPI"
     gene            complement(4885..5526)
                     /locus_tag="PLN75_01565"
     CDS             complement(4885..5526)
                     /locus_tag="PLN75_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRG02834.1"
                     /translation="MRKITTIILLLVTAISLNWQIHAQNPDIETLDRINSNEDKFLKG
                     YSTFMSESAIYVSFGVPAALGAISLLKHDDQMLKNAAYILAGQAVNTALTYSLKWTVD
                     RPRPYETYPHLIEPYKIMTSSSMPSGHTSLAFATATTLTLSYPKWYVIAPSYFWACSV
                     GYSRMNLGVHYPSDVAAGALLGAGSAYITHLVNCWLRQKTNNKKILTLQNYPF"
     gene            5702..6238
                     /locus_tag="PLN75_01570"
     CDS             5702..6238
                     /locus_tag="PLN75_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG02835.1"
                     /translation="MLKIAVIGPESTGKTELCRKLAVHFHAEWVPEMAREYVEKLEDQ
                     YTFEDVCSIARLQIEQEQKYGRKTKPDYIFFDTDLIVTKVWLAYKYDFVPDFVNERLA
                     SRFFDFYLICLPDIPWEYDPVRENGDKREFFYAWYENEINVLGTPYSRIGGSGEERLQ
                     NALQAIKVFETNRKSNTF"
     gene            6307..7185
                     /locus_tag="PLN75_01575"
                     /pseudo
     CDS             6307..7185
                     /locus_tag="PLN75_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
     gene            7535..8707
                     /locus_tag="PLN75_01580"
     CDS             7535..8707
                     /locus_tag="PLN75_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRG02836.1"
                     /translation="MEFEMVAKTFKGLEEVLAGELVELGANNVQLERRAVSFTGNLEM
                     LYKANLHLRTATRVLKTIAAFKAENPDEVYEKVKKIDWEKYMKVDTTFAIDSTVYSEV
                     FRHSRFVAYRVKDAIADFFTEKYDKRPSVRLDNPQLMINIHIAHNQCTLSLDSSGESL
                     HKRGYRASQTEAPLNEALAAGMILMTGWRGESDFLDPMCGSGTLLIEAALIALNIPPG
                     IFRSSFAFEKWNDFDEDTFQRLYNDDSLERTFEHKIYGSDNAPRAISIARQNVQSAGL
                     TKYIELAVSPLQELEAVSDTCLLVTNPPYGERITSPDIFGLYADLGSFLKHKFTGNKA
                     WVISSHDDCLFKIGLKPSRKIELLNGALECLYCEYEIFAGKRNEFVTKEKKRKREW"
     gene            9151..9993
                     /gene="kduI"
                     /locus_tag="PLN75_01585"
     CDS             9151..9993
                     /gene="kduI"
                     /locus_tag="PLN75_01585"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HRG02837.1"
                     /translation="MKTNYELRYAAHPEDAKAYDTKRLRRDFLIEKVFSADEVNMVYS
                     MYDRMVVGGAMPVNESLKLEAIDPLKQPVFLMSRELGIYNVGGKGSVKVGDDVFELDY
                     KEALYLGSGKREVFFESEDKNNPARFYFNSATAHRNYPDKKVTKADAVSVELGSLETA
                     NHRVINKMLVNQVLPTCQLQMGMTELAPGSVWNTMPAHVHSRRMEAYFYFEVPADQSV
                     CHFMGEVDETRHIWMQNNQAVLSPEWSIHSAAATSNYTFIWGMAGENLDYGDQDFSKI
                     NELR"
     gene            10095..10886
                     /locus_tag="PLN75_01590"
     CDS             10095..10886
                     /locus_tag="PLN75_01590"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HRG02838.1"
                     /translation="MNQFDLTGKVALVTGATYGIGFSIASALAEAGATIVFNDIKQEF
                     VDKGLKAYDEAGIKAHGYVADVTDEDAINEMVAKIENEVGVIDILVNNAGIIKRIPMH
                     EMTAAEFRQVIDIDLNGPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNICSEYGGHNIQCNGIGPGYIATPQTAPLREKQPDGSPHPFDSFILAKT
                     PAGRWGSTDDLKGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            10893..12032
                     /locus_tag="PLN75_01595"
     CDS             10893..12032
                     /locus_tag="PLN75_01595"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRG02839.1"
                     /translation="MKQTIIAFFVTALLVSCTNAKVEVTVANQLGMDRENEIVEISRN
                     ILWSKLNLSSTDRIIVQDESSRQVTYQITSNPEDETDSLLIFPAIVKANASSVYTIRK
                     GKPGVFQSKVAGRLVPERKDDFNWENDRVAFRMYGPALQATGEISNGIDLWAKRTDRL
                     VADKWYAEELSGKSTYHTDNGEGLDFYKVGPTLGAGAAAPYLNDQLWFSRNFTTYEIL
                     DKGPLRITFRLNYDAFNVDKLGEVSSTRIISLDAGSQLNKITQIHDFKAESMPVAAGI
                     VLRNSPEEQVSTDENKSWAAHAEPADSINGTLYQAIAAATPFTGIEKANNHLLGLQTV
                     KTNEPFIYYAGGGWSKYGFDTFEDWTKYVKEFTEKLQSPLTITIN"
     gene            12050..13075
                     /locus_tag="PLN75_01600"
     CDS             12050..13075
                     /locus_tag="PLN75_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRG02840.1"
                     /translation="MSKKIVTLGEIMLRLSTPGNTRFVQSDVFDVVYGGGEANVAVSC
                     ANYGHDAYFVTKLPKHEIGQSAVNALRKYGVKTDYIARGGDRVGIYYLETGASMRPSK
                     VIYDRAHSAIAEAKPSDFDFDAIMKGADWFHWSGITPAISNSAAELTKLACEAAKRNG
                     VTVSVDLNFRKKLWTTQKAQSIMRPLMQYVDVCIGNEEDAELCLGFKPDADVEGGHTD
                     AEGYKGIFTQMANEFGFKYVISTLRESFSASHNGWKAMIYDGKEFYTSKRYDIDPIID
                     RVGGGDSFSGGIIHGLLTKPNQGEALEFAVAASALKHTVNGDFNLVTIEEVEALAGGD
                     ASGRVQR"
     gene            13332..13691
                     /locus_tag="PLN75_01605"
     CDS             13332..13691
                     /locus_tag="PLN75_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRG02841.1"
                     /translation="MTSEELKLRFRKFSGRIIKMVDNLPNTISGKAIGAQVVRSGTSP
                     GANYRAACRAKSDKDFVNKLKIVEEELDETEYWIDIISDVELIKPELLLELKKETKEL
                     LAIITQSLITKKNKMNN"
     gene            13753..14421
                     /locus_tag="PLN75_01610"
     CDS             13753..14421
                     /locus_tag="PLN75_01610"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRG02842.1"
                     /translation="MMRFSKLQVMTAMKETGIVPVFYNSDTEVAKNVVKACYEGGIRA
                     FEFTNRGDFAHEVFGELVKFAAKECPEMIMGIGSIVDAPTAAIYIQLGANFVVGPLFN
                     PDVAKVANRRLIPYSPGCGSVSEVGFAQEAGCDVCKVFPGDVLGAAFVKGLKAPMPWS
                     NLMVTGGVKPEEANLKTWFDAGVMCVGMGSNLFPADMMKAGDWAGITKLCADAIAIVK
                     KVRK"
     gene            14450..15877
                     /locus_tag="PLN75_01615"
     CDS             14450..15877
                     /locus_tag="PLN75_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG02843.1"
                     /translation="MNNTQKMTNFRWTIVVLLFATITINYLDRQVLSLTWDEFIKPEF
                     HFGDDIYGFITGFFSLFYAVCYLFAGRFVDWIGTKKGFLWAIGIWSLGACVHAVCGIV
                     TESVVGLPDKSALINATGDTALVISTVGMWAFIVARSILALGESGNFPAAIKTTAEYF
                     PKKDRAFATSIWNAGASVGALASPLAIPPIAKAFGWELAFIIIGALGFIWMGFWIFLY
                     EKPEKSKYVNAAEVEYIDQDKHEQVAEKTEKIEKIPFLKCFTYKQTWSFAFGKFMTDG
                     VWWFFLFWMPLYIKAQFNIKTTDPTGQLLIFTMYAIITVLSIYGGKLPTIFINRSGQN
                     PYAARMKAMLIFAFIPLLALVAQPLGTYSYWFPVIIIALAGSAHQSWSANLFSTIGDM
                     FPKGAIASITGIGGMAGGLGSFFLQNTAGKLFIYSEQNNLQFLGFEGKPAGYFIMFCF
                     CAVAYLIGWTVMKALVPKYKPIVVE"
     gene            complement(16004..16828)
                     /locus_tag="PLN75_01620"
     CDS             complement(16004..16828)
                     /locus_tag="PLN75_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRG02844.1"
                     /translation="MNNQKVVLITGASSGIGKEAARLLSGKGYKVYAASRNLEKMEEL
                     KSSGVIPLSLDITDEHSIDACVAEILTAEKRIDILINNAGYGSFGPAEAIPLNEARRQ
                     FEVNLFGLAALIQKVLPVMRSQNSGRIINISSMAAYFSEPNGGWYHATKAALERYSDS
                     LRMEVKGFNIKVVLIQPGMIHTEWQVIAGQNLVKHAENTVYEKSARNQAAMFELGYKY
                     ASKPAVVARAIVRAATSKHPCLRYRVGGMAKPILFFRKITPDRWFDALVARLMIRN"
     gene            17297..20491
                     /locus_tag="PLN75_01625"
     CDS             17297..20491
                     /locus_tag="PLN75_01625"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRG02845.1"
                     /translation="MEFNGTDQYMQIPTHADFNVQTSESFTITAWVNVIEFKNNNARY
                     ITKRLQGTTVADKSGYELWGANSAAQYYALNTPNASNNHNNSLSVWPTYTGSLNKWTH
                     IAFVVDRAGGKMYEYIDGVEVANSGTKDISPWAVNNAWDVYVGCGITGTTAASPTQYF
                     RGQMDNLRFWKKALSPADIIADKTATVTAETAGLVAAYDFENINTTTLKVPDIKNAHE
                     ATLVNYTFSDKTIIKVTQDVNFTGRGNDNEEILRLEVTPAGTSIVELKELVMNMNGTT
                     DINDVDSIKIYTTGGVTRFDPRKPAGTLIGKAAAATGDITIPLTGMLNINANYLWVTY
                     KLKETASEGNKVDAVLVSLTTKDETVIPANGNPAGLRTILLRRTLIYGPGDYSSTNYR
                     IPAITTAADGSLVVLTDKRKLGSGDLAANIDVVANRSTDGGKTWSAPVTVALGTSSST
                     GFGDAAIIKANSGKLVALYVGGPGFFNSTPTVPIRSYISTSSDNGVTWSAPRDITSQI
                     YGAGCADPVRAAWQGSFFGSGHQLTLRNGRLMAVIAVREPSVSGIQNYAVYSDDEGET
                     WSVSKRALSGGDEAKVVELNDGSILMSVRTSGNRLWTKSTDGGVTWGAKNSWPEIWGN
                     ACDADIIRYTSTKDGFDKNRILHTLPNASDRTNVTMWVSYDEGSTWPVKRSIAAGQSA
                     YSSVTILPDGTIGVYLEEDGSIPYKMYFLNYSLEWLTNGADKWLPVNTLITSNPVFSL
                     AAGKYTEDKTLTITADEGATIRYTLDGSMPTKTSAVYTAPIQITRSMTVKAFAQKEGF
                     GNSSVTVADYIYGWDVPGQNRGTIDQRYLISAKTEGADVNLNFAASSPPATHFVSYNN
                     EIIEAKTGSTFTLKLNALEGQTDGLQWCQAIILIDWNRDYDFTDAGERIAIVGNRSID
                     NGAAVLNISQAIVVPVGAVVNQKTRLRVVYSDGWRPTSYTDYGFDPVDKGRIYDFDLK
                     IIASTGVEHVLADLLIAFPNPARNMVNLNMPVAGKYNVSLVTLDGKTIETKSVILNDA
                     AKYTIDLSSYTSAAYLVNVIHESGFEKSIRILKQ"
     gene            20665..20937
                     /locus_tag="PLN75_01630"
     CDS             20665..20937
                     /locus_tag="PLN75_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRG02846.1"
                     /translation="MNIENLYKLKSRFVARKVGNEVVVVPLVNNVAQMERLYTLNETA
                     GFLWENLNETATVESLKTALLENFDVEDSVAERDIQNFLENLKKLV"
     gene            21089..21949
                     /locus_tag="PLN75_01635"
     CDS             21089..21949
                     /locus_tag="PLN75_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02847.1"
                     /translation="MPHQLYLSIAGFSICLHAECQLMLDDGFTHFTVDSTQEADITIE
                     CRNNDSLPPFENAEKVFTSANDELKFYEIYRRGDDLIFRVFDQLNQTCIQQTALLDGG
                     LKRWIIWSEETDGVLEPLVFPMAPILLHYAVLTADAVMMHASGVFDGEKGRLFSGFSG
                     AGKSTISGIWKQQGNLLINDDRLIIRKEKGEYFMYNTPMYYPDDNKKSPLHAVFLIRH
                     APENVINRMAGAAAVSSVMAFSIQNNYDTRFVQHHLDFFANMCSQIPVFQLGFVPDEK
                     VVNFIKEKTR"
     gene            22197..22781
                     /locus_tag="PLN75_01640"
     CDS             22197..22781
                     /locus_tag="PLN75_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02848.1"
                     /translation="MFFEKDCIYHVYNRSNEKLFYSRENYLFFLKKIRFQVLPYADMI
                     AYCLMPNHFHLMLRANESGVETYDSSTKINVQKLSQGLGSLISSYTQALNIQRNRRGG
                     LFAHRTKAKLLNGVKDDYLLNCFLYIHQNPIEAAIVDKLEDWEFSSYLDYIGRRKGTL
                     VDVNQGLELLNISAADIKYIIDFKIPDISDENFM"
BASE COUNT         6795 a         4780 c         5121 g         6324 t
ORIGIN      
        1 ataatttgtt ataaaataaa tagaattatg taattttgtt gtcaattaat caacccttaa
       61 acatttttgt accatgactt acgatgaata cgagaaaatc aaaaaactgg aagaacttct
      121 gaaaaacgga accattactc aggaagaatt cgacaaggaa agggaaaaaa tcatcagcag
      181 tgccccaaaa gcagcacctt ctgaaaaacc ttttgctgcc agcaccaggc agccgcttat
      241 gggtattgaa gaaaatctgt acctgctgct tatgcattta tcacagtttc tcactgcctt
      301 cattattcct ctggtgatgt ggctggttgg gaaagatgaa aacaagcggg tggattttca
      361 tggaaaaaac attttgaatt tccagattac gtatattatc tggttgattg taggaattat
      421 taccattcct cttattatcg gtatagtcat tttatccgtg ttgggtattg ccatgacagt
      481 gtttattatt gtagctgccg tgaaatcata caacggtgag gactggaaat acccgctgac
      541 aatcaatttt ctgaaataat ttcaaggaat taatactgac agtaaaaaac gtgtgaggct
      601 tggaaacagt ttcatgcgtt ttgcgtttat atagccacgt atgatattta cagatataca
      661 cacccatcat tcgttgtccg aaccggacat tttaacagtt cgaaacatcc ggatggaaga
      721 agctgaaagt gtccttggta ctgctcaggg aggatttttc tcagtcggaa ttcatccatg
      781 gtatctccac aatatttctg ctgaggatat cagactactg gagttgattt gcagtgataa
      841 acgtgttgtg ctgataggag aatgcggaat cgataaaaac tcaacatttt cggccgaaat
      901 gcaaatccct gtttttgaaa agcaaatact tatttccgaa agaatctcaa aaccgctgat
      961 tgtacattgc gtgggcagtt tcaatgagtt gtttgtcttg cggaaaaaac accaaccaac
     1021 ccaacgatgg attatacacg gttttcgtgg caagccgcag ctggctgagc agatacttca
     1081 gtccggttgt gacatttcat tcggtgaaaa atttaatccg gaaagtgtaa gaatagtgcc
     1141 ttttgagcgc ttatttattg agacagatca aagcgagatt tccatcgaag aaatatatca
     1201 tcagattgcc gctgcaaaaa actgcaaaat tcatgaattg ttggcaggaa atttatttat
     1261 aaacgaattc tccagcattt tataaaaata tccaattgtt aaatctgttt tcagattatg
     1321 tttaaaaact aaataaaaga taagcctcac caaattttca tagctttgcc cgaaatttgc
     1381 gtttcaaatt aaaaaaatct catgaagaaa tatatctcac tcgctgtttt tctccttgct
     1441 gttttttcgg cacacccgca gggtatatca tcgatccagc agcgtaaact ggctcaggcg
     1501 ctgtctgcca tttcgggtat gtatgtggac accatcaacg acaaaaaaat catagaatcc
     1561 accattcgcg gagtgcttga agagctcgat ccgcattcca catatatacc gaaggaggaa
     1621 gttcagcgta tgcacgaacc gctcgaaggc agtttcgatg gtattggcgt gcagtttcag
     1681 attattaagg atacgatcaa cgtggtgcaa accgtatcgg gtactcctgc cgaaaaagtg
     1741 ggagtactgc ccggtgataa aatcgtgtat attgaaaatg aatcggttgc cggtataaaa
     1801 attcagaata ccgatgtttt caaacggctg cgcggcaaaa gaggaacacc tgtaaacatt
     1861 cgggtgaaac gtggtaatgc cgcggaactc atcgatttca aaatcatacg tgataaaata
     1921 cctatctaca gtgtggatgc ctcgtatatg ctcaccgata agattggata tatcaaaata
     1981 aacagtttcg gatcaactac aaccgaggaa tttaacaaag cgcttaaaac gctgaaatcc
     2041 aaagggatga aagacctggt gattagcctg gagcgtaacg ggggcggatt tcttaaaacc
     2101 tccatcgagc tggccgacga actactgcct gcaaaacagc tgatagtcta taccgaagga
     2161 ctgaatgctc cccgtaccga agccaaagct accgccgccg gaaactttga atccggacgt
     2221 gtggtaatac tcatcgatga gttttcggct tcggccagcg aaattctgag tggtgctgtc
     2281 caggactggg acagaggagt tcttattgga aggcggacgt tcggcaaagg tctcgtacag
     2341 cgggaagtat cgcttgccga cggctccatg atgcgtctga cggtagcccg ttactacaca
     2401 cctaccggac gcagcataca gaaaccgtat gtaaacggag gcgccaaaga ttacaacaaa
     2461 gatttggaag aacgctttga gaaaggcgaa atgatgcatc cggacagcat tcattttccc
     2521 gattcgctga aattcaatac actgaccgca aacaggactg tgtatggcgg cggaggcatc
     2581 atgcccgata ttttcatccc gatggataca accgaaaaca cgctttatca ccgccgcata
     2641 gtgggtaagg gagtagtcaa cagcaccatg atagaatatc tgaacataaa cagagatgaa
     2701 ctccgaaaaa aatatccctc gtttgatgca tttaataaga attacgaagc cgatgaatcg
     2761 ttgctgaaaa tcctcatcga caacgccgaa aaagagggca taaaacccga tgaaaaagac
     2821 tttaaccgct caaaacgagt aatcaaactg caactgaaag caatgatagc caatgatttg
     2881 tgggggctga atgaatatta tgaggtaatc aattcggata acaaatcgtt gcagaaagcc
     2941 gttgaaatac tcaccacaaa aggagcttac gataaaatgc tgggaaagaa aaagtagccg
     3001 ttaattttta catgataaag aattaatgaa tgcgtcagga tcacctcatc tgcaatttca
     3061 cccggatttt tttcaggtta aatgattcgt tgtcatgaaa gattatatta ctgaaaattg
     3121 ggtggaaatt gccggtgcag tgctgagtct gctgtacctc tatctgtcca ttcgtgagaa
     3181 atccgccctg tggattttcg gtttcatcag ttcgttgctg tatatttttg tttttttcga
     3241 aagcaagctc tacgccgata tgtcgcttaa tttttactac ctgggtgtga gcatttacgg
     3301 ctggatcaac tggcatcgaa aaactggtca gtttaaggat tccggactac atattacacg
     3361 catcagtggc aagcagcaaa tcctctcgta tcttttcggt actatattcg tttatctggc
     3421 atattacctc gtgctcgagc tccttactga ctccactatt cccgtagccg actcggtggt
     3481 gggtgcactc agtgtgatag ctacgtggat gcttgcccgg aagaagatag aaaactggct
     3541 ggtctggatt gtggtcgatg cttttgctgc gggattgtat ttatataaag gactgtatcc
     3601 caccaccata ctctttataa tttacacaat aatggctgtt gtaggttatt atcagtggaa
     3661 gaaaactccg gacgccatca aataggcgat ttcatgtcaa ggtacacatc cagcacctcc
     3721 agatcgcgtt ggataacgat atgcagcggt cttttgcggg gagtttcgag tatttttctg
     3781 atttcaacca ttttcatcgt aaaaaccggc tgtttgtcca cttccagtat taaatctccc
     3841 ggtttgatcc ctttcaggtc ggcggttgaa tttttccaca ctttatgcac tgtaatatca
     3901 aagatgtaag ggaaattttg tgtcatttcg atacccgaga tgttgtaact gaatttttcg
     3961 gcaaacatcc ggttgggttt cagataaaga gccggtgttt tgaaatcgat gaagctatcg
     4021 aaacgcttca gcacctgatt accgagtgtg ccgtctctgg atgagcggat tataaaatca
     4081 gctatgtaaa gcgaatcagg aaaagttacg atgggttttt tcaggtaaaa aggtcctatg
     4141 ttcaggctgt caattacggc gtaatacccg tgaatttcgc cgttcagacc ctcgccgatg
     4201 tatccgtgta ttctcttttc gggcagttcg aaagcatccg cgcggatagt ctgaaaccag
     4261 gcgccgattt cagcacccgt atctatcagc atggccaggt tttcatttgt tatcccattg
     4321 tttattacat tggcattgac atacatttta ctgccggcca caatggcggg tattcgcttg
     4381 tatgatcccg gcactgcgta tgactcgtga gtgtagaaag tgattctttg acgcgaatag
     4441 cttatttcca ctacataatc tttcagaatt tccgaaccga gtattccatt gattttttct
     4501 cccgtgatgg acgaaaaact gatgattttg tccataagca ccagaatgtc ctggttttga
     4561 agccgcagat ttcctatttt gagcatattt ttcgtgctgt ggtatgcccg gatgctgtca
     4621 ttgagtccca gaccgcgtat tcctacaatt tcaccgcgag gcaattcaac cgaatcttcg
     4681 ggtagaattt cggttatcat ggtgtttttt acgcctgtat caagtatgaa attaagcgga
     4741 gatgaaccgt tgatgctggt ctcaaccaca atggtgctac ctacaagtct gaatgggaaa
     4801 gaagccactt tcttctgtct gcagaatgca ggctgactta tcagcaccaa aagaagcagg
     4861 atgtaagcta ttcgtttcat gctgtcagaa aggataattt tgaagcgtca ggattttctt
     4921 gttgttcgtt ttctgccgga gccagcagtt caccaaatga gtaatgtagg cgctacccgc
     4981 acccagcaag gctcctgcag ccacatccga aggataatga acgcccagat tcatgcgcga
     5041 gtaacccaca gagcatgccc agaaatacga cggagctatg acataccatt tcggatagct
     5101 gagtgtgagc gtggtagcgg tggcaaaagc cagcgaggta tgacccgatg gcatggagct
     5161 ggaagtcatt atcttataag gttctatcag atgtgggtag gtctcgtagg gacgtggtct
     5221 gtccacagtc catttcaatg aataggtcag tgcggtattc acagcctgac cggccaggat
     5281 atatgcggcg tttttcagca tctggtcgtc gtgtttaagc aaagaaattg ctcccagcgc
     5341 tgctggtaca ccaaaagaaa cgtaaatggc cgattcggac ataaacgtag aataaccttt
     5401 caggaatttg tcctcattgc tgttgatccg gtcaagcgtt tctatatccg gattttgagc
     5461 atgtatctgc caatttaaag agatggctgt gaccaaaagc agaataattg ttgtaatttt
     5521 gcgcatcgtt acgttttttc tattacaaat atagattaat ttttccggca aacgttcaac
     5581 atgtttcaaa taatcatcag attcctttca gaaggaaaaa gttcattatc aatcagcaaa
     5641 ataaatacga ttgccgcgaa tgaaaatctt tgaattttaa taaatacatt tttctaagaa
     5701 aatgctaaaa atagccgtga taggacccga atcgactgga aaaacggaac tttgcagaaa
     5761 acttgccgtt cattttcatg cggaatgggt tccggaaatg gctcgtgaat acgtagaaaa
     5821 acttgaagac cagtacactt ttgaggacgt ttgcagcatt gcccgcttgc aaatagaaca
     5881 ggagcaaaaa tacgggcgaa aaacgaagcc ggattatatt ttttttgata cggatttgat
     5941 cgtaaccaaa gtctggctgg cttataaata tgattttgta cccgattttg tgaacgaaag
     6001 attagcctcc cgtttttttg atttttacct gatttgcctg ccagacattc cctgggaata
     6061 cgacccggtg agagaaaacg gagataagcg tgagtttttt tacgcctggt acgaaaatga
     6121 aatcaacgta ctgggtacac cctattcccg catcggaggc tccggagaag aaaggcttca
     6181 gaatgccctg caggctataa aggtatttga aacaaacaga aaatccaata cattctgaac
     6241 acatcattca gatctgttgt ttgattatct tcaggaattt tttaaaaaac aaaaaaacta
     6301 ctctcaatga ataatgaaat aaaacgtaca atagctcaac tttcagatcc tgaagggttg
     6361 aagtacgtat gtcgtcgtca ggaagaaaat caccccatgc catcgaccga aattatcaat
     6421 aaaatcgtaa aactgcttcg ctcggtcata tttccgggtt atttcgggaa tgcggatgtc
     6481 aatcctgaga atgtgagtga gcatattgaa gaaaatgtac aactgataca tgctctgtta
     6541 cttcagcagg ttaagtcggg aatgtgtttt gaaaattcag aacttgattt tcattgcacc
     6601 gaactgaatc aaaaagccga aagcctgact gccgatttta tttccggatt gccgcagctg
     6661 cgcgatatgc tccacaaaga tgcagtagct tcgttcaacg gcgatcccgc ctccaaaagc
     6721 ctgggcgaag tgatattttg ctatcccggc attcgggcca tcagcagcta ccgcattgct
     6781 catgaattgc tgcaactggg tgttccaatt attccacgag tcattaccga actagcccat
     6841 tccgaaaccg gcatcgatat tcatccggga gcaactattg gtgactcttt tatgattgat
     6901 cacggcacgg gtgtggtaat cggcgaaaca gcccggatag gcaaaaatgt tcgtatttat
     6961 cagggagtaa cactgggtgc caaaagtttt ccgctcgata aaaacggcaa tcccatcaag
     7021 ggaatcgacc gccatccgaa gattggcgac aatgtgatca tttattccaa ttccaccatt
     7081 ctgggaaata tccaggtagg cgaaaatgca gttatcggag ggaatatatg ggtggatacc
     7141 gatgtgccgg caggaacgaa ggtgcttcag cgtaaaaggg tggaacagga agcaacaaac
     7201 ggtgatacaa aacggagaaa agataaaaaa cagaaaacgg aaagaaaaaa agacaagccg
     7261 gaaaaacaga aagataaaca gaaagaaaaa tatgcagcgg aaaggatcag atatgttttt
     7321 gacccgaaaa ccgacaaacc cattcctccc tttgccaaat gcaaaggctg caaaagcaaa
     7381 aacggatgtt caggctgccg cgccaaatac gagaaaaaat acggagtaaa agcgtgaatg
     7441 attcccgaaa ataagccgga gagaagaaaa aaaaacagcg tgaaaaaagg aaccggtaaa
     7501 acgggataaa aaaaatattc aaaatcagaa agatatggaa tttgaaatgg tagccaagac
     7561 cttcaaaggt ctcgaagagg tactcgcagg agaactcgtg gagttgggag ccaacaatgt
     7621 gcaactggag cggcgtgccg taagttttac cggtaatctc gagatgctct ataaagccaa
     7681 tttgcacctg cgtactgcaa cccgcgtact caaaaccatt gctgcattca aagcagaaaa
     7741 ccccgacgag gtgtacgaaa aggtaaaaaa aatcgactgg gaaaaataca tgaaggttga
     7801 cactaccttt gccatcgact caacggttta ttcggaggtt tttcgtcatt cccggtttgt
     7861 ggcttaccgc gtaaaagatg ccattgccga tttttttacc gagaaatacg ataagcgtcc
     7921 gtcagtaagg ctggacaatc ctcagctgat gattaacata cacattgccc acaaccagtg
     7981 taccctctcg ctcgacagct caggcgaatc gctccacaag cgcggataca gggcatcgca
     8041 gaccgaagca cctctcaacg aagcgcttgc cgcaggcatg attctgatga ccggctggcg
     8101 gggcgaatcc gatttcctcg atcccatgtg cggctcgggt acgttgctta ttgaagcggc
     8161 actcatagcc ctgaatatac ccccgggcat tttccgcagt tcttttgctt ttgaaaaatg
     8221 gaacgatttt gacgaagata catttcagag actttacaac gacgatagcc tggagcggac
     8281 attcgaacat aaaatttacg gttcggacaa tgcgccgcgc gccatcagca ttgcccgcca
     8341 gaatgtacag agtgccggac tgaccaaata catcgagctg gctgtcagtc cgttgcagga
     8401 actggaagct gtttcggaca cctgcctgtt ggtgaccaat ccgccctacg gcgagcgaat
     8461 tacttctccc gatatattcg gtttgtacgc cgatttaggc agtttcctta aacacaaatt
     8521 caccggcaac aaagcctggg tcatcagctc ccacgatgat tgcttgttca aaatcggatt
     8581 gaaaccatcc cgcaaaatcg aactcctcaa cggagcactc gaatgcctgt attgcgagta
     8641 cgaaattttt gccggcaagc ggaatgagtt tgtgacaaaa gaaaaaaaac gaaaacgcga
     8701 atggtaatct catttatttt gtttattttt gaaaaaattc agttgaaaaa caaggcttta
     8761 aattccgctt tgaaaattca actgaatttc tgaaattagt tgcatttctt tgaaaatctt
     8821 ttccccggga aaaataaatc gatattaatc acgatacttt gaacttcgtt tttttaataa
     8881 aaagcattaa aaacgtcatg tcgtgtttct tcttgtaaat aatttttact acttttgtga
     8941 aaattgaaat ttataagaaa acaccagttg acgaatgaat cattcgtttt tttaagttta
     9001 tattttgtgc acgtgcacgg attgtttgat tttaaaagat tattttttca aaacaggtaa
     9061 acactacaca tttttatctc acttttccat gtgtcaggcg gattttctcc tgactgaatc
     9121 ataaattaat gtaaaataaa atatacgatt atgaaaacga actacgaact gcgctatgca
     9181 gctcacccgg aggacgcgaa agcgtatgac acgaaaagat tacgccggga ttttttgatt
     9241 gaaaaagttt tttcggcaga cgaggtaaat atggtttatt ccatgtacga ccgtatggtg
     9301 gtaggtggag ccatgccggt caatgaaagt ttgaaactcg aagccattga tccgttgaaa
     9361 cagcccgtat tccttatgag tcgcgagttg ggcatttaca acgtgggagg taaaggttcc
     9421 gtgaaggtag gcgacgatgt tttcgaactt gattacaaag aagctttgta tcttggttca
     9481 ggcaaacgcg aagtgttttt tgaaagtgag gataaaaata atcccgcccg cttctatttc
     9541 aattcggcca ctgcacaccg caactatccg gataagaaag tgacaaaagc tgatgcggtg
     9601 agtgtggaac tcggttcgct cgaaacggct aatcacaggg taatcaacaa gatgctggtc
     9661 aatcaggtgc tccccacctg ccagttgcag atgggcatga ccgaactggc acccggaagt
     9721 gtgtggaata ccatgccggc gcacgtgcat tcacgccgga tggaagctta tttctatttt
     9781 gaagtgcctg ccgatcagtc agtttgccat ttcatgggcg aagtggacga aacccgccac
     9841 atctggatgc aaaacaatca ggcagtactc tcgcccgaat ggtctattca ctcggcagca
     9901 gctacttcca attatacctt catctggggt atggccggtg aaaacctgga ttacggcgat
     9961 caggatttct ccaaaatcaa cgaattaaga taaaattcca tcaaaggaag ttgtttctta
    10021 aactaacctt cgttattcaa acgatttata taaactaact tactataaat tatttactat
    10081 aaactaacct caatatgaat caatttgatt taactggtaa agtggcactt gtaacgggag
    10141 ctacttacgg tattggattt tctattgctt cggcgctggc tgaagcaggt gctaccattg
    10201 ttttcaacga catcaaacag gaatttgttg ataaaggatt gaaggcatat gatgaagccg
    10261 gaataaaagc acatggatac gtagccgatg tgactgacga agatgctatt aacgagatgg
    10321 ttgcaaaaat cgagaacgaa gtgggtgtca ttgatattct ggtaaacaat gccggtatca
    10381 tcaaacgcat cccgatgcac gaaatgacag cagctgagtt tcgtcaggtg attgacattg
    10441 acctcaacgg accttttatt gtatccaaag cagtaatccc gtcgatgatt aagaagggtc
    10501 atggtaaaat catcaatatt tgctccatga tgagcgaact gggtcgtgaa accgtatcgg
    10561 cctatgcggc tgccaaaggc gggttgaaaa tgcttaccaa aaacatttgt tcggaatatg
    10621 gcggacacaa cattcagtgc aacggtatcg gaccgggcta cattgccact cctcaaactg
    10681 ccccgcttcg cgaaaaacaa cccgatggtt ctcctcatcc gtttgattca ttcattcttg
    10741 ccaaaactcc cgccggacgc tggggttcaa ctgacgacct gaaaggaccg gccgtattcc
    10801 tcgcttcaga tgcttccaac ttcgtaaacg gacatattct ctatgttgat gggggaattt
    10861 tagcttacat aggcaaacaa ccgtaacgaa atatgaaaca gacaatcatt gcttttttcg
    10921 taactgcgct gctggtttct tgtaccaacg caaaggttga ggttaccgtg gccaaccaac
    10981 tcggcatgga ccgtgagaat gaaatcgtgg aaatcagcag aaatatcctc tggtcgaagc
    11041 tgaatctgag ttccaccgac cggataattg tacaggacga gtcatcccga caggtaacct
    11101 atcagattac atccaatccg gaagatgaaa ccgactcact gttgattttt ccggcaatcg
    11161 taaaagccaa cgcttcatct gtctatacca tccggaaagg aaaacccggt gtttttcaat
    11221 ccaaagtggc cggacgcctt gtaccggaac ggaaagatga tttcaactgg gaaaacgacc
    11281 gtgtggcatt ccgcatgtac ggtcccgcgc tgcaggcaac gggagaaata agcaacggca
    11341 tcgacttgtg ggcaaaacgc accgaccggt tggtggccga taaatggtat gccgaggagc
    11401 tttcaggaaa atccacctac cacaccgaca acggtgaagg actggatttc tataaggtag
    11461 gacccacact tggcgcaggt gcagcagctc cttatctgaa cgaccagctt tggttcagtc
    11521 gcaatttcac aacatatgaa attcttgaca aaggaccgtt gcggattaca ttcagactga
    11581 attatgatgc ttttaatgtt gataaactcg gtgaagtgag ttcaacacgt attatctcac
    11641 tcgacgcagg cagtcagctg aataaaatca cacagattca tgacttcaaa gccgaaagca
    11701 tgccggttgc tgccggtatt gtattgcgaa attcacccga agaacaggta agcaccgacg
    11761 aaaataaaag ttgggcagct catgccgagc cggcagactc catcaacgga accttgtatc
    11821 aggcgattgc tgccgccaca ccgtttaccg gcatcgaaaa agccaataac cacctgttgg
    11881 gattgcaaac cgtcaaaaca aatgagccat tcatctatta cgccggaggt gggtggagca
    11941 agtatggttt cgatacgttt gaagactgga cgaagtacgt gaaagaattt actgaaaaac
    12001 tgcaatcacc actgacaatc accattaact aatttttttt ttgaatagaa tgagtaaaaa
    12061 aattgtaaca ttaggagaaa tcatgctccg tttgtcaaca cccggaaata cacgctttgt
    12121 acaatcggat gtatttgatg tagtatatgg cggcggcgaa gccaatgtag ccgttagctg
    12181 tgctaattac gggcatgatg cttattttgt aacaaaactg cctaaacacg aaatcggtca
    12241 gtcggcagta aatgcactgc gcaaatacgg tgtaaaaaca gattacattg cccgtggcgg
    12301 cgatcgtgtt gggatttatt acctcgaaac cggcgcttca atgcgtccga gcaaggtaat
    12361 ttacgaccgc gctcattcag ccattgccga agcaaaacca tccgatttcg attttgatgc
    12421 cattatgaaa ggtgctgact ggtttcactg gtcgggaata acgcctgcaa tttctaattc
    12481 agctgctgaa ctgacaaagt tagcctgcga agctgcaaaa cgaaatggcg ttaccgtatc
    12541 ggttgacctg aatttccgca aaaaactatg gacaactcaa aaggcacaat caatcatgcg
    12601 tccgttgatg cagtatgttg atgtttgcat cggaaatgag gaagatgccg aactttgtct
    12661 gggttttaaa cccgatgccg atgttgaagg cggacacacc gatgccgaag gctacaaagg
    12721 tatttttact caaatggcta acgaatttgg atttaaatat gtgatttcta ctcttcgcga
    12781 atctttttca gcaagccaca acggttggaa agccatgatt tacgatggaa aagaatttta
    12841 cacctcaaaa cgatatgata tcgaccctat tatcgaccgt gttggtggtg gcgattcatt
    12901 ctccggtgga attattcacg gattattgac taaaccaaat cagggcgaag cactggaatt
    12961 tgcagtagca gcttcagctt tgaagcatac tgtaaacggt gattttaatc ttgtaacgat
    13021 agaagaagtg gaagcattgg ccggtggcga tgcaagcgga agagtacaac gttgatgtac
    13081 aatttaaaaa tgtacaattt acgattgaat aaaacgacat tgtgcattga caagaaacaa
    13141 cgttgatgta caatttaaaa atgtacaatt tacgattgaa tgaaacgaaa tctctatatg
    13201 caaatacttc aattgtaaat ttttaaattg taaattgtac atattaacaa aagaaaatct
    13261 ctatatgcaa atgacgcaat tgtaaatctt taaattgtaa attgtaaatt caaaaaattg
    13321 tacataaaaa aatgacttca gaagaactta aactcagatt cagaaaattc tctggcagaa
    13381 ttatcaaaat ggttgataac cttccaaata caatttcagg taaggcaatt ggagcacagg
    13441 ttgttcgttc gggtacttcg cccggagcaa attacagagc agcctgtaga gctaaatctg
    13501 ataaagactt cgtcaataaa cttaaaatag ttgaggaaga acttgatgaa actgaatatt
    13561 ggattgatat tatatctgac gttgaattaa ttaaaccaga acttcttttg gaattgaaaa
    13621 aagaaacaaa agaacttctg gcaataatca ctcaatcact tattactaaa aaaaataaaa
    13681 tgaacaatta atcaattgga aaaaggtttt ttcacccaat tgtaaattgt aaatttttaa
    13741 atcgtacata aaattatgag attcagcaaa ttacaggtaa tgacagctat gaaagaaacg
    13801 ggaattgttc cggtttttta taatagcgat acagaagtag caaaaaatgt ggtaaaagca
    13861 tgctacgaag gtggtattcg tgcttttgaa tttactaacc gcggcgattt cgcacacgag
    13921 gtttttggcg aattggtgaa atttgcagca aaagaatgtc ccgaaatgat tatgggcatc
    13981 ggttccattg tggatgctcc cacagcagcc atttatattc agctgggagc caattttgta
    14041 gtgggaccgc tttttaatcc cgatgtggca aaagttgcca accgtcggtt gattccctat
    14101 tctccgggtt gcggttcagt ttcagaagtt ggatttgcac aggaagccgg ctgcgatgtt
    14161 tgcaaagttt tccccggcga tgtgttgggt gctgcttttg taaaaggact gaaagcgcca
    14221 atgccatggt cgaacctaat ggtaactggc ggtgtaaaac ccgaagaagc caacctgaaa
    14281 acctggttcg atgcaggcgt tatgtgtgta ggaatgggct caaacctttt cccagccgac
    14341 atgatgaaag ccggcgactg ggcaggcatt acaaaactgt gtgccgatgc aattgcaata
    14401 gtaaaaaaag taagaaaata attattaata acaatcaaaa aattatatca tgaacaacac
    14461 tcaaaaaatg actaatttca gatggacgat agtggttttg ctgtttgcaa ctattaccat
    14521 aaattatctc gaccgtcagg tactttcact aacatgggat gaatttatca aacctgaatt
    14581 tcatttcggc gatgatattt acggtttcat taccggtttc ttttctctgt tttatgctgt
    14641 gtgttattta tttgccggac gttttgtcga ctggataggc accaagaaag gtttcctttg
    14701 ggctatcggt atttggtcat tgggtgcatg tgttcacgca gtttgcggaa ttgtcaccga
    14761 atctgttgtt ggattacccg acaaatcagc ccttattaat gccaccggcg atacagcgct
    14821 tgtgatttca acagtaggaa tgtgggcttt tattgtagca cgaagtattc ttgctttggg
    14881 tgaatcggga aattttccgg cagccattaa aactacagcc gaatattttc ccaagaaaga
    14941 tcgcgctttt gccacttcaa tctggaacgc aggtgcatct gtaggcgcat tggcttcgcc
    15001 gttggctatt ccacctattg ctaaagcttt cggttgggaa ttggcattta ttattattgg
    15061 tgcattggga tttatttgga tgggtttttg gatttttctt tacgaaaaac ctgaaaaaag
    15121 taaatacgtg aacgctgcgg aagtagaata tattgatcag gacaagcacg agcaggttgc
    15181 tgaaaaaacc gagaaaattg aaaaaattcc ttttctaaaa tgttttacct ataaacaaac
    15241 atggtcattt gctttcggta aatttatgac agacggtgtt tggtggttct ttttattttg
    15301 gatgccactg tatatcaaag cccaatttaa tattaaaacc accgatccaa ccggacagtt
    15361 attgattttt acaatgtacg caataattac agttttatca atttacggtg gaaaattacc
    15421 aactattttc attaaccgca gcggacaaaa tccttatgca gcgcgaatga aagccatgtt
    15481 gatttttgcc tttatacctt tattagcact tgttgcacaa ccactcggaa cttattccta
    15541 ttggtttcct gtaattatta tcgcattggc aggttctgct catcagtcgt ggagcgctaa
    15601 cctgttttca accatcggcg acatgtttcc aaaaggagcg atagcatcaa ttaccggaat
    15661 tggaggtatg gcaggcggtt tgggttcatt ttttctgcaa aatacagcgg ggaaactttt
    15721 tatctattct gagcaaaata atttgcaatt tcttggtttt gaaggcaaac ccgctggata
    15781 ttttattatg ttctgctttt gtgcggtagc ttaccttatt ggttggacag tgatgaaagc
    15841 acttgtgcct aaatacaaac caattgttgt ggaataataa atatgcccac taacccccga
    15901 aggggtacta acttcaaaga acggagtata taaacagaaa aatcagcggg attcaagaag
    15961 ggaatttcgc tgatttttct gtttatatta atgggtaagt gtcttaattc ctgatcatca
    16021 gtcgggcaac cagggcatca aaccacctgt cgggagtgat ttttcggaaa aagagaatcg
    16081 gtttagccat accgccgacc ctgtaacgca ggcacggatg cttacttgtg gcagctctga
    16141 caatagctcg tgctaccacc gcaggttttg atgcgtattt gtaacccagt tcaaacatgg
    16201 cagcctgatt tcgtgctgat ttttcgtaca ccgtgttttc agcatgtttc accagatttt
    16261 gacccgctat tacctgccat tcggtatgaa tcatcccggg ttgaataagc accactttta
    16321 tattgaaacc ttttacctcc atccgaagcg aatccgagta acgctccaaa gcagctttgg
    16381 tagcatggta ccacccgccg ttgggttcgc tgaagtaggc agccatggat gatatattga
    16441 tgatgcggcc gctattttga ctacgcatca cgggcagcac tttctgaatg agggcagcca
    16501 gtccgaacag attcacttca aactgccttc gggcttcatt cagggggatg gcttcggcag
    16561 gcccgaacga cccgtatccc gcgttgttaa tcaaaatgtc aatacgtttt tcggcggtaa
    16621 gtatctcagc tacgcatgcg tcgatggaat gttcatccgt aatatcaagc gacaaaggaa
    16681 tcactcccga tgatttcagt tcctccattt tttcgaggtt tcgggatgct gcatatactt
    16741 tgtacccttt tccggataag agtcttgcag cttcttttcc aatgcctgat gatgctccgg
    16801 tgattaatac aaccttttgg ttattcatac attcgtgaaa aattatcttt ttttttagtt
    16861 tattaaattg aatattctgc aataatttcc ctcaaaaaaa ttgtgaaatt tatgcctgaa
    16921 attgagctat aaacgtttaa cgccatggaa ttgttcatcg ccggtacgcc ttttttgtta
    16981 ccggttacag cacaggagaa gttgtttata tgaaaaaaaa ccggcaggta cccgcatgaa
    17041 tttatcggga atgaatttta ttgagtatgg tttttttaat gccgtttccg aaaaagcagg
    17101 agtggatgtt tttctgtcat ttttgaaagt tatatcgtat aattatttta aaacaatcgt
    17161 atagttataa ataatattta ttatatttgt gccaaattta atttttactt ttataataca
    17221 tacaacaatg aagcggattt ttaccatttt tatgctggca ctgccattta tcgtgttttc
    17281 acaaacaggc aaatagctgg aattcaacgg aacagatcag tacatgcaaa tccctacgca
    17341 tgctgatttc aatgtacaaa catccgaaag ctttaccatt actgcctggg tgaatgtaat
    17401 cgagttcaaa aacaacaatg cccgatatat tacaaaacgt ctgcagggta caactgtagc
    17461 cgacaaatcg ggttacgaat tgtggggagc aaattcggca gctcagtatt atgcactgaa
    17521 tacaccaaat gcatctaaca accacaacaa ttcactttcg gtatggccaa cctacaccgg
    17581 ttcactcaac aaatggacgc acattgcttt tgtggtggat cgcgcgggag gaaaaatgta
    17641 tgagtacatc gatggtgtag aagtggcaaa ctccggcacc aaagacattt cgccctgggc
    17701 cgtaaacaat gcctgggacg tgtatgtggg ttgtggaatt acaggaacta ctgcagcttc
    17761 accaactcag tattttcgcg gacaaatgga caacctccgg ttctggaaaa aagcgctttc
    17821 gcctgccgat atcattgccg ataaaactgc aactgtgaca gctgaaaccg ccggactggt
    17881 agcagcctac gatttcgaaa acattaacac caccacgctg aaagttccgg atataaaaaa
    17941 cgcacatgag gcaacactgg taaattacac gttcagtgat aaaactatca taaaagtcac
    18001 tcaggatgta aactttaccg gacggggcaa cgataacgag gaaattctcc gactggaagt
    18061 tacccccgcc ggaacttcca tagtagaact gaaagaactg gtgatgaaca tgaacggtac
    18121 taccgacata aacgatgtag acagcataaa aatatacacc acaggtggag taacacgttt
    18181 cgatccgcga aaaccagccg gcacgctgat aggaaaagca gccgcagcca ccggagatat
    18241 taccataccg ctcaccggaa tgctgaatat taatgcgaat tatttatggg ttacctataa
    18301 actgaaagaa acagccagcg aaggcaacaa agtggatgct gtcctggtct cgttgactac
    18361 caaagacgaa actgtaatcc ctgccaacgg caatcctgcc ggtttgcgta caatattatt
    18421 gcgccgtacc ctgatttacg gacccggcga ctatagctcc accaactacc gcattccggc
    18481 tattaccaca gcagccgacg gctcgctcgt agtgcttacc gataaacgta aactcggttc
    18541 aggagacctt gcggccaaca tcgacgtggt ggccaacaga agtaccgatg gcggtaaaac
    18601 atggtctgct cctgtaacgg ttgcactggg aacaagctca tcgacaggat ttggtgatgc
    18661 agccattatt aaagccaaca gcgggaaact ggtagcactt tacgtgggtg gtcccggatt
    18721 ctttaattca actccaactg ttcctatccg atcttatatc tctacaagta gtgataatgg
    18781 tgtaacctgg tcagcgccac gtgatataac ctcacagatt tatggtgcag gttgtgccga
    18841 tccggttcgg gctgcatggc aaggatcttt ctttggctca ggccatcagt tgacgttgcg
    18901 caatggccga cttatggctg taattgctgt aagagaaccc tccgtaagtg gtattcaaaa
    18961 ctatgccgtt tactccgacg acgaaggtga aacctggagt gtttcgaaac gtgctctatc
    19021 gggaggcgac gaagccaaag ttgtggaact gaacgatggc tccatactca tgagtgttcg
    19081 cacaagtggc aaccgcttat ggacaaaatc caccgacggt ggcgtaacct ggggagcgaa
    19141 aaattcgtgg cctgaaatct ggggcaatgc ctgcgatgcc gatataattc gttatacttc
    19201 aacaaaagat ggatttgata aaaacagaat tcttcataca ctccccaatg cttctgatcg
    19261 tactaatgta accatgtggg ttagctacga cgaaggttca acatggcctg ttaagagaag
    19321 tattgcagcc ggtcagtcgg cttattcctc agttacgatt ctgcccgatg gaacgattgg
    19381 tgtttacctc gaagaagatg ggtcaatacc ttataaaatg tatttcctta attattcgct
    19441 cgaatggctg accaacggtg ccgataaatg gcttcctgtt aatactctca ttacttcgaa
    19501 tccggtattt tcgttggctg ccggtaaata tactgaggat aaaacactca ccattactgc
    19561 cgatgaagga gcaactattc gctatactct tgatggatca atgccaacca aaacttcggc
    19621 tgtttatact gctccgattc agattacacg ttcaatgaca gtgaaagctt ttgcacaaaa
    19681 agaaggtttt ggtaatagtt cggtaactgt tgccgattat atttacggat gggacgtacc
    19741 cggacaaaac cgtggcacaa ttgaccaacg ttacttaatt tcagctaaaa cggaaggtgc
    19801 ggatgtgaat ttgaatttcg cagcttcatc tcctcctgct actcattttg tttcgtacaa
    19861 caacgagata attgaagcta aaaccggttc gactttcaca cttaaactga atgcactcga
    19921 aggtcagacc gatggacttc agtggtgtca ggcaattatt ctgatcgact ggaaccgtga
    19981 ttacgatttt accgatgccg gagaacgaat tgccattgtc ggaaaccggt cgattgataa
    20041 cggtgctgct gttcttaata tttcacaagc tatagtagtg cctgtaggag cagttgttaa
    20101 ccagaaaacc cgcctacgtg tggtttactc tgatggttgg cgtccgactt cctacaccga
    20161 ttatggattt gatccggtgg ataaggggcg tatatacgat ttcgacctga aaataatcgc
    20221 ttcaaccggt gttgagcatg tactggccga tctgctgatc gcttttccga atcctgccag
    20281 gaacatggta aacctgaata tgcctgtagc cggaaaatac aacgtatcac ttgttacatt
    20341 ggatggaaaa acgattgaaa caaaaagtgt tattctcaat gatgcagcaa aatacaccat
    20401 cgatctgtca tcctatactt cagctgctta tctggtaaat gtgattcatg aaagcggatt
    20461 tgaaaaatca atcagaatat tgaagcagta aaaatagttt ttcctctgta aaaataaaaa
    20521 agagaaatgc agcaatgtct ttctcttttt tgttattcag gccgctattc atggcgtgac
    20581 tccaggtcac cccgtgaata acaacgcggg gagaggcttc ttttttctga aaattcgcta
    20641 tctttgtaaa aaaaagaaca caatatgaat atcgaaaatc tgtacaaact caaatcgcgg
    20701 tttgttgccc gtaaggtggg aaacgaagtg gtggtagtac cgctggtaaa taatgtggcg
    20761 cagatggaaa gactctacac cctcaacgaa actgccggat tcctgtggga aaatctgaac
    20821 gagaccgcta cggttgaatc gctgaaaacc gcactccttg aaaacttcga tgtggaagat
    20881 tccgtagctg aacgggacat tcagaacttc ctggaaaatc tgaaaaaact cgtatagtca
    20941 gggttttgag ttctgtcttg cccgaggcag acagggttca aggttccgtg ttctgtattc
    21001 tgaattctgt gttccgtgtt ctgaatatca gcttttaatc atgcatccca atcagttcat
    21061 tcttttcaga tgataaaaaa acatttctat gccacaccaa ctatatcttt ccattgcggg
    21121 tttttcaatt tgccttcatg ctgagtgtca gctgatgttg gatgatggat tcacgcattt
    21181 taccgttgat tctactcagg aagctgatat tacgatcgaa tgcaggaaca atgattcgtt
    21241 gccgcctttt gaaaatgcag agaaagtttt cacttctgcc aatgatgaat taaaattcta
    21301 cgagatttat cgtcggggcg acgacctcat tttcagggta ttcgaccagc tgaatcaaac
    21361 ctgcattcag cagactgctc ttctggatgg cggactgaaa cgctggatta tctggtcgga
    21421 agaaacggac ggcgtgcttg aaccgttggt tttccccatg gcgcccattt tgctgcatta
    21481 tgccgttctc acggccgatg cggtgatgat gcatgcctcc ggcgtgttcg acggagagaa
    21541 aggccgactc ttcagcgggt tttccggtgc gggcaaaagt actatctccg gtatctggaa
    21601 gcagcaggga aacctcctca tcaacgacga ccggcttatt atccgtaagg aaaaagggga
    21661 gtatttcatg tataacacac cgatgtatta tcccgatgat aataaaaaat cgccccttca
    21721 tgccgtgttt cttatccggc atgcacccga aaacgtgatt aaccggatgg caggtgctgc
    21781 ggcagtaagc agtgtgatgg ctttcagcat tcagaataac tacgataccc gtttcgtaca
    21841 gcatcatctc gatttctttg ctaacatgtg cagtcagatt ccggtatttc agctcggatt
    21901 tgtgcccgac gaaaaggtgg taaactttat taaagagaag acacggtgag gatatctctg
    21961 tttctgcacc cggtccaacg gcttctgcac ccggtccaac ggcttcaggc cgtttgaccg
    22021 attaagaagg aagcggtgct gagtttgtga aacgcaacaa accgcttaga gcggcagtgc
    22081 agattccggg taacgcaaca aaccgcctaa agcggttggt tgctcgtgct gagcggttgg
    22141 ttgctcgtgt tgcttcaggc cgtttgaccg attaagaatg taaatgaata tgtaatatgt
    22201 ttttcgaaaa agattgtatc tatcacgttt ataacaggag taatgaaaaa ctgttttatt
    22261 ccagggagaa ttatcttttc ttccttaaga aaatcagatt tcaggtattg ccttatgctg
    22321 atatgattgc ttattgcttg atgccaaatc attttcatct tatgctgagg gcaaatgaat
    22381 cgggtgtcga aacgtatgat tcatccacta aaataaatgt tcagaaatta tcacaagggt
    22441 tgggtagtct tataagttcc tacacgcagg cattaaatat tcagagaaac agaaggggag
    22501 gccttttcgc acacagaaca aaagctaaat tgcttaacgg ggttaaagat gattatttgt
    22561 tgaattgttt tttatacatt caccaaaatc caattgaagc agcaattgtt gataaactcg
    22621 aagattggga gttttcgtcc tatcttgatt atatcgggag aagaaaaggt acgctcgtag
    22681 atgtaaatca agggcttgaa ttgttaaata ttagtgcagc tgatattaaa tatatcattg
    22741 attttaaaat tcctgatata tctgatgaga atttcatgta acttgcatcc ggtccaacgg
    22801 tttctgcacc cggtccaacg gtttctgcac ccggtccaac ggcttctgca cccggtccaa
    22861 cggcttcagg ccgtttgacc gattaagaag gaaaaagcgg cagtttgctc gtgctgattc
    22921 cgggtaacgc aacaaaccgc ttagagcggt tggttgctcg tgctaagcgg ttggttgctc
    22981 gtgctaagcg gttggttgct cgtgctaagc ggttggttgc
//
LOCUS       DAORKP010000013        22688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_81_length_22688_cov_8.902753, whole genome shotgun sequence.
ACCESSION   DAORKP010000013 DAORKP010000000
VERSION     DAORKP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22688
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81_length_22688_cov_8.902753"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1345)
                     /locus_tag="PLN75_01645"
     CDS             complement(77..1345)
                     /locus_tag="PLN75_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRG02849.1"
                     /translation="MLKFLFKGLIRDSSRSLLPVIVVTLGVMITVVLQSYMTGVLGDS
                     IETTANFTTGHVKVMTAAYSKNMSQFPNDLALMDVDTLIRKLQSDYPDVAWAPRIQFG
                     GLLDVPDENGETRTQGNVAGIGIDMLQSGREIDRMNLRSNLTGGRFPEKPAELLISEE
                     LFRKMKLKTGDEVTLIASSMYGEMALYNFRVCGTLRFGVPFLDRGTIIADISDVQTAL
                     NMENSAGEILGFFKNQYYNNESAASMAKTFNMNQKNDDKFAPVMLPLCDMNGMGYIVA
                     YSENIQAVILLIFIIAMSIVLWNAGLIGGLRRYGEFGLRLAIGESKNEIYRTMIGEAF
                     LTGVIGTIFGVGIGLFLSWLLQHYGINIGEMMKDSTILMPTVLRARITTASYYIGFIP
                     GIVSTVIGAMLAGIGIYKRQTSNLFKELES"
     gene            complement(1384..2520)
                     /locus_tag="PLN75_01650"
     CDS             complement(1384..2520)
                     /locus_tag="PLN75_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRG02850.1"
                     /translation="MGNGLKTWLNVFVLSISFVLIVFMQALLEGWSKQAVTDAVAWEI
                     GGGQYWSSKYDPYDPFSLDSCAAPLPSILNDAFEKHQIEPVLITPGSIYPDGRMMPVL
                     IKGIRPDQNMLVLPTQALNGDSMEVPVIIGAHMASQAGLKENDLVTLRWRDANGTFEA
                     VDVRIASVFKTTVPTTDNGIIWISLENLQRMMSQPGKATLLIKNKELATQNLPGWKFM
                     DQTELTKQTVALVKAKSVGMSIFYFIFLFLALVAIFDTQTLSIFRRQREIGTYVALGM
                     TPRQVQHFFTLEGTMNALLAVALGFVYGLPFFIWFAANGIAMPVDVSNFGLPIADKMY
                     PLFTPKLIIGTIIFIFLITALVSYLPARKISRMNPTEAIRGKVL"
     gene            complement(2723..3424)
                     /locus_tag="PLN75_01655"
     CDS             complement(2723..3424)
                     /locus_tag="PLN75_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG02851.1"
                     /translation="MNRIVEIKNLVKNFPIGNDVFTALNGINLEFREGEFAGLVGPSG
                     SGKTTLLNIIGSLDVPSGGEVTVLSHSISSLSQKEAAQLRKEKLGFIFQNYNLLPVYT
                     VYENVEFPLLLLNFSTDERKKRILETLEWVGLSDKINSRPAQLSGGECQRVAIARAMV
                     KRPSLVLADEPTANLDSANSHNVLQTMVKLNRELKTSFIFATHDDKVIGYLHRIIRLE
                     DGKVVDDRPSTPNRV"
     gene            complement(3513..4712)
                     /locus_tag="PLN75_01660"
     CDS             complement(3513..4712)
                     /locus_tag="PLN75_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02852.1"
                     /translation="MKQTCLILIFSFAFLCHAQLKTDSLPAASAEFPNDSTTDKNSFS
                     LNGQITGWTIFEFSDPTHTQAAGRFVPVFTGIQHLKGSSRLDFEASLNIQGALHFTGN
                     KFDSATYVFKPYRVWLRYAGNNWEIRGGLQKINFGTARMFRPLMWFDQMDVRDPLQLT
                     DGVYGVLGKYFFANNLNIWLWTLMGNKNPKGFEMFGSAAAIPEIGGRIETPLGPGEMA
                     FSTHFRKADLHTAIPAVAEKTYINERRMGLDGKWDIGPGIWFEASVSMFEKTDLLFFR
                     FQDMWNAGADYTLPIGAGLNVTAEYFRLHFGDKILKEGTTVNFFGSMITYPVSIIDNV
                     SAMVFYAKETGKFFNYISYARTYDNWNFYLIGFWNPENNLPIGIKPGGRNLFAGKGVQ
                     LMASFNF"
     gene            4868..5329
                     /locus_tag="PLN75_01665"
     CDS             4868..5329
                     /locus_tag="PLN75_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRG02853.1"
                     /translation="MKKQYTFEELLHKAASYCSISEHCASEVEQKLRVWEVEEADRKK
                     IIAQLTADDFINEKRYAKAFVRDKFRLSKWGKIKITMALREKGIEKDIINNALNTIDE
                     GEYDEMLASLLKSKVKTLKFEFEYEKQGKLFRFAQSRGFENQVIERVLRYI"
     gene            complement(5404..6249)
                     /locus_tag="PLN75_01670"
     CDS             complement(5404..6249)
                     /locus_tag="PLN75_01670"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRG02854.1"
                     /translation="MSTQQHIDFERIARAIEYMYTHSASQPDLAKTASQVHMSAFHFQ
                     RMFQEWAGVSPKKFLQFLTVNHAKEMLKKSPVSLTEAAERVGLSGTGRLHDLFLNLEG
                     MTPGEYKNGGINLKISYSYTLSPFGRMLIASTGRGVCYLAFADDGQQAYNELIELFPK
                     AEFTAQSDPQIEKVIRFFEDNMGNPAKITLHLKGTEFQLKVWETLLKIPEGKLASYGE
                     IANLIDNPKASRAVGSAVGDNPVSYLIPCHRVICSTGIPGQYHWGAARKKAMIGWEAA
                     RGMMP"
     gene            complement(6453..8807)
                     /locus_tag="PLN75_01675"
     CDS             complement(6453..8807)
                     /locus_tag="PLN75_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRG02855.1"
                     /translation="MLNKNKSWWYFALILGIFGVVAYFLIRHGNAIDVALHRSVAEGT
                     EGTLELFKNLTLHNLMEPAAMILLQIIAILIVSRIFSAVFAKIQQPTVIGEILAGIVL
                     GPSLLGMIFPEAYAFLFSPDSLDNLYIISQIGLVLFMFAVGMDLNMSELKDKIRNTFI
                     ISQSSIVIPFMFGMILAFVIYKDFSPSNSNFTSFALFIGISMSITAFPVLARIVQEKG
                     LSKTHLGTISIGSAAINDVTAWILLVAVIAIAQTGSFASSVYTLIFTAIYIVVMLFVV
                     RPFLKQIGAIYQKEELMNKSIFGFFILILIISAYTTQLLGIHALFGAFMAGLIMPPMP
                     RFRKLMVDKIEDLSVSLLLPLFFVYTGLKTEIGLLNTPSLWLMCLVFIVVAVAGKLGG
                     SAIPARAIGESWKDSLSLGVLMNTRGLMELIVLNIGLEMGILPPVVFVMLVIMALVTT
                     FMTTPFLALIEKLFPDKTVEKELIRQQSEGIFKTLIALGNPEGGKNLLRVAKKVLDGV
                     KNSLEVEVLHITAGTDTNPIYSEQFSTESFVDVLAEAKTLQIPVTTDYKVADIIEAEI
                     VKKVNTENFDFLLVGGGVSTTQSAILRESSKFYKFGWLSRIANKLTRDNALFYPGTLI
                     RDKTRFFIEHSNCSVGVFVNRDFKGISNILVVIEKETDDFLLRYARRILRNNAETTVQ
                     IIDLEKMNGKVHSITDGINKLITQYGSRVKVARLTRLHSGYLEKFSLMLISYDSWEYL
                     VSQDIQTLKDIPSTLVINKKESRFNQNIHIPETAVIEPGKDTEL"
     gene            complement(8963..10042)
                     /locus_tag="PLN75_01680"
     CDS             complement(8963..10042)
                     /locus_tag="PLN75_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRG02856.1"
                     /translation="MLDQIYYNNTLQNWLISGGIIAFALIINWIITLLNRRYLKALAK
                     RTKNQVDNILVNTLETPLKVGIVLIAIWTALNRLELSKSFDEALYKTYEILTVLNITW
                     FVAHLASRLIREYFIKKSEQEKDIRQKIHFDSHLTSLIRKTAVFGIWTVGTVTALNNV
                     GLDLKAILGTLGIGGIAIALAAQDTVKNIFGGFTILMDGTFRIGDRVKIGEFDGIVED
                     IGIRSTKIRNFDKRLITLPNYKIMDNAVMNVSAAPQLRVVSNLGLTYDTTPELMQKAL
                     EILNNIALKNEAVSNEGISAVFESFGEFSLNIRYCYYVKDAGDTFDTPSAINFEILKK
                     FNEAGISFAFPTQTLHIEKTIVNAP"
     gene            complement(10158..12098)
                     /locus_tag="PLN75_01685"
     CDS             complement(10158..12098)
                     /locus_tag="PLN75_01685"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRG02857.1"
                     /translation="MEKLTTSPSKGGFRGAFGFVRVAAAIPELRVADCAFNALKINEL
                     IVNAAAQNVEIICFPELCITAYTCADLFFQKQLLDDAEKALEKLVKETAELNILSIVG
                     MPVRKNNQLFNAAVVFGRGEILGVIPKINLPNNNEFYEKRWFASGKTVTAGYIQLAGQ
                     ETNFGTKYYFHTTDFTFGIEVCEDLWMPVPPSSYLAIQGAEILFNLSASNELIAKHIY
                     RQQLVEQQSARCIAGYVYTSAGHGESTTDLVFSGNTIIVENGKVLAKGERFSFEPQLT
                     ISEIDVERLRADRMRNSNFFNEREEDAYFEIDCRKNDPAPENPDFKLNRKIDQHPFVP
                     PVSVRDESCHEIFSIQVAGLAKRWQHTRLDKLVIGISGGLDSTLALLVCVKTADKLGY
                     DRKRILGVTMPGFGTTDRTYNNAIELMKALGITLMEINIKEACLLHFKDIGHNPEVHD
                     VTYENVQARERTQILMDLANKHNGMVVGTGDLSEMALGWSTYNGDHMSMYGVNSGVPK
                     TLVRYLVGWAAHQLDDQSEKILTDILDTPVSPELLPFDKNGDIAQKTEEIVGPYELHD
                     FFLYYFVRFGFTPEKIHFLAKNAFSEKYSDETIDKWLKLFLKRFFQQQFKRSCTPDGP
                     KVGSINLSPRGDWRMPSDAVSF"
     assembly_gap    12255..12354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12355..13550)
                     /locus_tag="PLN75_01690"
     CDS             complement(<12355..13550)
                     /locus_tag="PLN75_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRG02858.1"
                     /translation="MYLNTEIAKDIYYVGVNDRIKHKFENMLPLPYGVSYNSYLIVDE
                     KVALVDTVEISFGDVFIDKIQSQLRDRQIDYLIINHMEPDHSGSLSLLRKYYPNVTLV
                     GNKKTLEMVQGYYGIDDNTLEVSDGEILKLGTHVLQFHLTPMVHWPETMMTYDESEKV
                     LFSGDAFGCFGTLDGGVIDSEMNLDKYWDEMYRYYANIVGKYGAPVQKALQKLTPLDI
                     KIICSTHGPVWKEQTSKAVDIYDQLSRYEAKEGVVVVYGSMYGNTERMAEAVAQGLVQ
                     NGIRNVVMHNVSVSDASVILRDIFKYKGLIIGSPTYCNDLYPEIHSLLTKIEIRQVKK
                     RDFAFFGSYSWAGITNKKLAEFGEKMKWRMIDFTVEEKMGLTENNYQACIMLGRKTAE
                     LLKTPES"
     gene            complement(13898..16165)
                     /locus_tag="PLN75_01695"
     CDS             complement(13898..16165)
                     /locus_tag="PLN75_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG02859.1"
                     /translation="MKKKILLLCPAILIAGYAFAQTTPVHYNDTLREVVVSASRWEQE
                     IKNVPSRVIGISSKSNEFTNPQTTADMLQNTGQIFVQKSQTGAGSPVLRGFETNRILL
                     VVDGIRMNNAIYRGGHVQDVITLDNAILDKTEVLFGPSSTLYGSDALGGVIHFYTKNP
                     KLNRKSGNAYVRYSSANSEKTGHLDYNLGFGKIASLTSVTYSDFGDQRIGKSGDFGTF
                     GDFGKNLYNVTRVDNKDVVSTNPNPYIMAGSGYKQIDVLEKILYQQNEHVSHMLNLQY
                     SNNIGNLPRYDRMLELSYDKTTKTMLPTYAEWNYGPQKRFLGAYTLDINKQNRWFDDA
                     KFILSFQSLDQDRISRKLNKTNRKTQMEDVSVFSANFDLMKKISERTTLYYGLEETLN
                     LVQSTVTNIDITTGANASPAATRYPDGGNSWNSAALYVQGNHTLSQIFSLTGGLRLTS
                     VYMKSQFKNDRSAGNAIFPFDKAEINEIAPCGNLSVVANLPSKTKISAIVSTGFRAPN
                     IDDMVKFFDNTSSTTVVVPNTGLKPEYAYNAELNIVQRLGHTGKIEAGAYYTLLDNAL
                     TVQPTRFNGSDSVVVDGTSLKAMSIQNSNSGRIYGYYVGATMNPVRNLSLNGSVTATY
                     GKYTNEQKEVVPMDHIPPMYGKIGASYNFSKLKLEGFMRFSAAKKLADYSPSGEDNLS
                     QTAVTAVNAKGEATAWAGTAAWYTLNFRAAYDVTKHINLNAGIENILDRHYRQFASGV
                     SAPGRNIYGTLRVNF"
     gene            16630..18576
                     /locus_tag="PLN75_01700"
     CDS             16630..18576
                     /locus_tag="PLN75_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HRG02860.1"
                     /translation="MIVCIAEKPSVGRDIAEILGARTRKDGYMEGNGYQVTWTFGHLC
                     GLLEPAEYTPDWKIWALSRLPMIPKPFGIKVVENEGIQKQFRIIEKLISGADEVINCG
                     DAGQEGELIQRWVMQKASCKCPVKRLWISSLTEEAIREGFSKLKDQSEYQRLYEAGLS
                     RAIGDWLLGMNATRLYTLKYAQNRQVLSIGRVQTPTLALIVNRQLKIENFKPEAYWEL
                     KTIYRETSFSATAGRFSSVEEGQNFLKKVENAPFEVTDITTKKGIENAPRLFDLTSLQ
                     VECNKKFGYSADDTLKTIQSLYEKKITTYPRVDTTYLSDDMYPKIPDILRGLKDYELL
                     IQPLLAAKLPKTKKVFDNSKVTDHHAIIPTGVQPANLSAEEKNVYDLVARRFIANFYP
                     ECKVATTTVSGKVEDVEFKTSGKQILGEGWRVVFARPTAEEKEENGEEEEKTLPLFTK
                     GESGPHEPSLTEKWTTPPKPFTEATLLRAMETAGKLVENDELRDALKENGIGRPSTRA
                     AIIETLFKRNYIRKERKNLIATETGVELIQIIQEELIKSAELTGQWERKLRLIEKGEY
                     KASDFLDELKEMIIRIDLNVKSDNRTKAQLIAESRLAENPDMMIGTTCPVCGKGRIIK
                     GKTAYGCSEWKNGCTFRKTIVVAP"
     assembly_gap    18715..18814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18815..20332)
                     /locus_tag="PLN75_01705"
     CDS             complement(<18815..20332)
                     /locus_tag="PLN75_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRG02861.1"
                     /translation="MKKLTTFLLLISFVLTLSAQKQLKREMRAVWIATVANIDWPSNP
                     DLTPTQQRNEMREMLDRLQKSGINAIVLQIRPTADSFYPSDLEPWSYYLNGKQGKRPS
                     PYYDPLQFIIEEAHRRCMEVHGWINPYRVTNTAKQINQLDKDHLYFKNKNLFVEYGGK
                     HYFDPGLDETRDFLNKVVADIIMRYDIDALHFDDYFYPYPVGGRDFPDEKTFRENPRG
                     FSPDKKADWRRNNVDMVISELQKTIKDLKPWVQFGVSPFGVWRNDNMDPQGSATRAGI
                     TNYDDLYADILKWLKEGTIDYIVPQLYWEIGKKVADYEVLIKWWSDNTYGKNLYIGLY
                     ASGLYENKTPAWKNGNELARQLKMNQQYPKVEGAVYFSAKTFNRNPKGLQDTLQNNYY
                     KFPALQPIPKNIKGEASAQPQNLRILKDGKEAFLLWDEVDEEGGCEVMYYVVYGFKGK
                     EVGDMNDPANIVGLTTETCLDLREIDRKFKGRTTFVVTAVNRFKWESTPTHGVSRR"
     gene            20466..21398
                     /gene="prmC"
                     /locus_tag="PLN75_01710"
     CDS             20466..21398
                     /gene="prmC"
                     /locus_tag="PLN75_01710"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRG02862.1"
                     /translation="MQQFLKDLHTSLQNVYSESEIHVLGMLILEKLTGFSRTRLLTDK
                     EIILNETQRELSIHFLERLKKHEPIQYILGETEFCGLKFKVSPSVLIPRPETEELVEW
                     VKPLPQSSLPLSRYVGTSLDFPHKGEENPPFGGWGGFLDIGTGSGCIAVALKKKFPSV
                     NVTAMDISAEALALAKENAALNEVYIEFIQDDILHPTATNRKWDVIVSNPPYIPHAGK
                     AEMASGVLDFEPHLALFVPDDDPLLFYRRIAAFALEHLTSQGRLYFEIHQSLGRQCCL
                     LLESMGFRSVELRKDLSGNDRMISSIPPAKAGGN"
     gene            21805..>22688
                     /locus_tag="PLN75_01715"
     CDS             21805..>22688
                     /locus_tag="PLN75_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02863.1"
                     /translation="MKTTRFYVIIAAFLFTAVSCVENSQKYKAAVAERDSLATEKLML
                     DSSYNQTLALLNDIETGFAEITQTEKQMKLNLQSVEGPKVNRRQLIAAQMSAIKQGIE
                     QNKARIAELEQLASKNGKINTVLTETVKRLQSQMEEKGLQIKSLQAELDQKNIKITEL
                     TTTVTQQSKSIAEQSRSIEEQQQALEKQESTIKQQDTEMNAVWYCIATPKELKAAKII
                     TDGGLFQSKKVLSSEFDNSMFAQVDQRSAVSIPTYSKKVKILSQHPVNSYTLVTGEDK
                     NLTIEITDPARFWSVSKYL"
BASE COUNT         6087 a         5040 c         4996 g         6365 t
ORIGIN      
        1 ataaattgat ttgaaattac gtttctttaa gtcccccaag ggggattccc cgataaatcg
       61 ggacacggta ggggccttag ctctccaact ccttaaacaa atttgacgtc tgccttttgt
      121 aaatgcctat acctgcgagc atagctccta tcactgtcga tacgattccg ggaataaatc
      181 cgatgtagta agaagccgtt gtaatgcgtg cccggagtac tgtcggcatc agaatggtag
      241 agtctttcat catctcaccg atgttaattc cgtaatgctg cagcagccac gaaagaaaca
      301 gtccgatacc aacgccaaaa attgttccga tgacacctgt cagaaaagcc tcaccaatca
      361 tggtgcggta aatttcattt ttgctttcgc caatggcaag ccttaatcca aattcgccgt
      421 agcggcgcaa tccgcctatc agaccggcgt tccacaagac aattgacatg gcgatgatga
      481 aaatcagcag tataacggcc tggatattct cagaataggc cacaatgtac cccataccgt
      541 tcatgtcaca caacggaagc atcacggggg cgaatttatc gtcgtttttc tgattcatat
      601 tgaatgtttt tgccatcgat gcagcgcttt cattgttgta atactgattt ttgaaaaaac
      661 caagaatttc acctgctgaa ttttccatgt tcagcgctgt ctgaacgtca cttatgtctg
      721 ctatgatggt tccgcgatcc agaaatggta caccgaatct gagtgttccg cagaccctga
      781 agttatacag ggccatctcg ccgtacatcg aacttgcaat cagtgtgact tcatcacccg
      841 ttttcagctt catttttcgg aaaagttcct cgctgataag cagttccgcg ggcttttccg
      901 gaaatcttcc tccggtgagg ttggagcgca ggttcatccg gtcaatttct cgtccggatt
      961 gaagcatgtc aattcctatg ccggcaacgt tgccttgtgt acgggtctcg ccgttttcat
     1021 cgggcacatc cagcagcccg ccaaactgaa ttcgcggtgc ccatgccaca tccggataat
     1081 ccgactgcaa ttttcttatc agcgtatcca catccatcaa tgccagatcg ttggggaatt
     1141 gcgacatgtt tttgctgtaa gcggccgtca tcactttcac atgtccggtg gtgaaattgg
     1201 cagtagtctc tatgctgtca cccagtacgc cggtcatgta ggattggagc actaccgtta
     1261 tcattactcc cagagtgacc acaatcacgg gtagtaaact ccggctgcta tcgcgaataa
     1321 gtcctttgaa taaaaatttc aacatgataa atcgttgatt gaatgtcctt taaacatgat
     1381 atattataaa acctttcccc ggattgcttc agtcggattc atccttgaga tttttcgtgc
     1441 aggcagataa ctcaccaaag cagtaatcaa aaagatgaag attatagttc cgattatcag
     1501 tttcggggtg aaaagcggat acattttatc agctatcgga agtccgaaat tacttacgtc
     1561 cacgggcatg gcgataccgt tggcagcaaa ccagataaag aacggaagcc cgtatacaaa
     1621 acccagcgca actgcaagca gtgcattcat cgtaccttcg agtgtgaaga aatgctgcac
     1681 ctgccggggt gtcataccca gtgccacgta agttcctatt tcgcgctgtc ggcggaaaat
     1741 cgacaacgtt tgtgtatcga aaatggctac gagcgccaga aaaagaaaaa tgaagtaaaa
     1801 aatactcatc ccgaccgatt tggctttgac gagtgcaacg gtttgtttgg taagttccgt
     1861 ctgatccatg aatttccatc caggcagatt ttgtgttgcc aactccttat tttttatcag
     1921 caaggtggct ttaccgggtt gagacatcat ccgttgcagg ttttcgagtg atatccagat
     1981 tattccgttg tcggtggtgg gaactgtggt tttaaaaacc gatgctatcc tcacatctac
     2041 ggcttcaaaa gtgccgttgg catcgcgcca gcgcagtgtg accaggtcgt tttctttcag
     2101 accggcctgg ctggccatgt gagctccgat aatcaccggt acttccatac tgtcaccatt
     2161 aagggcttga gtgggcagca caagcatgtt ttgatccggt cgtattcctt ttatcaatac
     2221 tggcatcatt cgtccatcgg ggtaaatact ccccggggtg attagtaccg gttctatctg
     2281 atgtttttcg aatgcatcat tcagtataga cggtagagga gcagcacacg aatcgagcga
     2341 aaacggatcg tagggatcgt atttgctgct ccagtattgt ccacccccta tttcccaggc
     2401 tacagcgtct gttacagcct gtttgctcca tccctcgagc agtgcctgca tgaaaacaat
     2461 gagtacaaac gatatcgata gcacgaatac gttcagccag gttttcagcc cattgcccat
     2521 caggcttttt atggcagttt tgagaagaaa aaagccccct aaccccctaa agggggaatt
     2581 ttccccaggt gtttgaatga cacctaagtt ggggttgtct ctatctttgt ttattttgtt
     2641 catatttcag attttcagca ttcaactgta tttttttgct ctcagcaatt tatagctttt
     2701 tgcaggatgg attggaattt tcttataccc tattaggggt cgagggtctg tcatccacca
     2761 ctttgccgtc ttccaggcgt atgatccggt gtaggtaacc gatcacttta tcatcgtgag
     2821 tggcaaagat gaaactggtt tttagttccc tgttgagttt taccatcgtt tggagtacgt
     2881 tgtgcgagtt ggcagaatcc aggttggcgg taggttcgtc agccagcacg agcgaaggac
     2941 gtttcaccat ggcgcgggca atagccaccc gctggcattc acctccggat aactgtgccg
     3001 gacgtgaatt gattttgtcc gataaaccca cccattcaag cgtttccaga attcgtttct
     3061 ttcgttcatc ggtgctgaaa ttcaacagca atagtgggaa ttccacattt tcgtaaacgg
     3121 tataaaccgg caggagattg tagttctgaa aaataaaacc cagtttctcc tttctcaact
     3181 gagcagcttc tttttgcgaa agcgagctga tggagtgact caaaacggtt acttcgcctc
     3241 cggaaggtac gtcgagcgaa ccgataatgt tgagcagtgt agtcttgccc gaaccgctcg
     3301 gaccaaccag tccggcaaat tcaccttcac gaaattcgag atttatgcca ttcagagcgg
     3361 taaaaacatc atttccaatc ggaaaatttt ttaccagatt tttaatttcg actattctat
     3421 tcattgtcag gggtgtattg agccatttga aagttaatat acaaatgtat gaaaaaagtc
     3481 tcctttcaca gaggcaggga atctgttttt ttttagaaat taaaacttgc catcaattgt
     3541 actcctttac ctgcaaacag atttcttcct ccgggtttta tacctatcgg aaggttgttt
     3601 tcgggattcc agaatccaat gaggtagaaa ttccagttgt cgtaagtgcg tgcatagctg
     3661 atgtaattga aaaatttacc ggtttctttc gcgtagaaaa ccattgccga aacattgtca
     3721 ataatcgaaa ccggataggt aatcatcgaa ccgaaaaagt tcactgtggt gccttctttc
     3781 aggattttat ccccgaaatg cagacgaaaa tactccgctg ttacgttcag tcctgcccct
     3841 atcgggagtg tgtaatctgc tccggcattc cacatatcct gaaagcggaa aaatagtaaa
     3901 tcagttttct caaacatgct tacacttgcc tcaaaccata ttccggggcc aatatcccat
     3961 tttccgtcca atcccatccg gcgttcatta atatacgttt tttcggctac agcagggatt
     4021 gctgtatgta aatccgcttt tctgaagtgg gtggaaaaag ccatttcacc gggtccaagt
     4081 ggcgtttcta ttcgtcctcc gatttcaggg attgctgctg cacttccgaa catttcgaat
     4141 cctttcggat ttttatttcc catcagtgtc cagagccata tgttcaggtt gttggcgaag
     4201 aagtattttc ctaatacgcc atacacacca tctgtaagtt gcagtggatc acgcacatcc
     4261 atctggtcaa accacatcag gggccgaaac atcctggccg tgccgaaatt aattttctgc
     4321 aaacctccgc gtatttccca gttattgccg gcgtagcgaa gccatacccg atagggcttg
     4381 aatacatacg tagccgaatc gaatttattt ccggtgaaat gaagagctcc ctgtatgttg
     4441 agcgaagctt cgaagtccag tcttgaactg cctttcagat gttgtattcc ggtgaaaacc
     4501 ggaacaaatc tgcccgctgc ctgcgtgtgg gtaggatcgg agaactcaaa aatagtccat
     4561 ccggtaattt ggccgttgag tgaaaaactg tttttgtcag tggttgaatc gttgggaaat
     4621 tcagcggatg ccgcaggcag cgaatccgtt tttagctgtg cgtggcagag aaaagcgaat
     4681 gaaaaaatca atatcaagca agtttgcttc atgaactttt cttaaagaaa aattactacc
     4741 ctgtatgaac cgtaaaatta taaatattta tcgaaaaagc aataaaacat gaaatttagt
     4801 ctgcttgcat ttcatttccc tgaaataatc attacttttg tgagttcaat gtaataaaac
     4861 aattgagatg aaaaaacagt acactttcga ggaattactc cataaagctg catcatactg
     4921 ttcgatatca gagcattgcg cctctgaggt agaacagaaa ctgagagtct gggaggtaga
     4981 agaagcggat agaaagaaaa taatcgctca gctgactgct gacgatttta tcaacgaaaa
     5041 acggtatgca aaggcatttg tacgtgataa attccgtctg agcaaatggg gaaagattaa
     5101 aattacgatg gcactgcgtg agaaaggtat tgaaaaggat attattaaca acgctttgaa
     5161 taccatcgac gagggcgaat acgatgaaat gctggcttcg ctgcttaaat cgaaggttaa
     5221 aacgctcaag ttcgaatttg aatacgaaaa acagggtaaa ctctttcgtt ttgctcaaag
     5281 ccgcggtttc gaaaatcagg tcatagagag ggttttgaga tatatatagg taatgttcaa
     5341 ccgtttttta gactcaaatt cacaaaaacg tattctaaac tcaaaagcca tcttttttag
     5401 ggcttagggc atcattcccc tcgcggcttc ccagcctatc atggctttct tgcgggctgc
     5461 accccagtgg tattgacccg gaattccggt ggaacagatt accctgtggc agggtatgag
     5521 gtacgaaacc ggattatcgc ccacggccga tcctactgcc cgcgatgctt tcggattgtc
     5581 aatcagattg gctatttcac cgtaggatgc aagttttcct tccggaattt tcaacaatgt
     5641 ttcccaaact ttcagctgaa attcagtgcc tttcagatga agtgtgattt tggcgggatt
     5701 tcccatatta tcttcaaaaa atcgaatcac cttttctatc tgtgggtcac tttgtgctgt
     5761 aaattcagct ttcgggaaaa gttctatcag ctcgttgtaa gcttgctgcc cgtcgtctgc
     5821 aaatgcaagg taacagacgc ctctgccggt agaggcaatt agcatcctgc caaacgggct
     5881 taaggtgtat gaatagctga ttttcagatt tatacctccg tttttatatt cgccgggtgt
     5941 cattccttcc aggttcagaa agagatcgtg cagccgcccc gtacctgata aaccaaccct
     6001 ttcagctgcc tccgtaaggc ttacagggct ttttttcagc atctctttgg cgtggttgac
     6061 ggtgaggaat tgcagaaatt ttttaggact tacgccggcc cattcctgaa acatccgctg
     6121 aaaatgaaat gcactcatgt gtacctgtga agctgttttg gctaaatcag gctgagaggc
     6181 agaatgagtg tacatatact caattgcccg ggcaatccgt tcgaaatcaa tgtgttgctg
     6241 tgtggacatg cggtgtgtgt tttttttaca aaagtaatgg cggaggactt aaaatacaac
     6301 ccgattcttg ctgtctattc agaatacaaa ttagaaatta gaaattagaa attagaaatt
     6361 agtcccgata gctatcggga cagaactcag aacccagaac ccagaactct cccgatagct
     6421 atcgggacag aactcagaac tcagcactaa atttacaact ctgtgtcttt tccgggttct
     6481 atgactgcgg tttccggaat atggatgttc tgattgaaac ggctttcctt tttgttgatt
     6541 accagtgtcg aaggtatgtc tttcagtgtc tgaatatcct gtgataccag atactcccag
     6601 ctgtcgtaac tgatcaacat cagactgaat ttttctaaat aacccgaatg caggcgggta
     6661 aggcgggcaa cttttactct cgaaccatac tgtgttatca gtttgttgat accatcggtg
     6721 atggagtgca cttttccgtt cattttttcc agatcaataa tctgcacagt ggtttcagca
     6781 ttgtttctca aaatgcgccg tgcataccgg agcaggaagt cgtcggtttc tttttctatc
     6841 acaacgagaa tattggaaat tcccttaaag tctctgttga caaaaactcc cacactgcaa
     6901 ttgctgtgct caatgaaaaa tcgggtttta tcgcgtataa gcgttcccgg atagaacagg
     6961 gcattgtcgc gggtcagctt gttcgctatc ctgctcagcc agccgaattt ataaaacttc
     7021 gaactttctc ttaaaatggc cgattgtgtg gtagatacac cgcctccgac caatagaaaa
     7081 tcaaaatttt cggtgttcac tttttttaca atttctgctt cgataatgtc ggcaaccttg
     7141 tagtcggttg tcaccggtat ttgaagcgtt ttggcttctg caagtacatc tacgaaactc
     7201 tcggtcgaaa attgttcgct gtaaatcgga ttcgtatcgg ttccggctgt gatatgcagc
     7261 acttccactt cgagcgaatt ttttactccg tcgagtactt ttttagccac acgtagcaaa
     7321 tttttacccc cttcgggatt accgagtgca atcaaagtct tgaaaatgcc ttccgactgc
     7381 tggcggataa gttctttttc gaccgtttta tcggggaaaa gtttctcaat aagtgccaga
     7441 aacggagtgg tcataaaagt agttaccaat gccatgatta ccagcatgac gaacactacc
     7501 ggcggcagaa ttcccatttc aagtccgata ttaagcacaa taagttccat caatccacgt
     7561 gtattcatca gcaccccgag cgaaagactg tctttccagc tttcgccaat ggctcgggcc
     7621 ggaatggcgc ttccacccaa ttttccggca acggctacta caatgaaaac cagacacata
     7681 agccagagtg acggtgtatt gagcagacct atttcagttt tcagaccggt ataaacgaaa
     7741 aatagcggca gaagcagcga aaccgacaaa tcctcaattt tatctaccat cagttttcgg
     7801 aaacggggca tgggcggcat aatcaatccg gccataaaag ctccgaaaag tgcgtgaatt
     7861 ccaagtagtt gggtagtgta tgccgatatg atgagtatta aaatgaaaaa tccgaaaatg
     7921 cttttgttca tcagctcttc tttctggtag atagctccga tctgtttgag gaacgggcga
     7981 acaacaaaaa gcatcaccac aatgtaaatg gccgtgaaga tgagcgtgta aaccgaactc
     8041 gcaaaactgc ctgtctgagc aatggcaata acggctacca gcagaatcca tgcagtcaca
     8101 tcgttgatgg cagcactgcc gatactgatg gtgcccaagt gagttttcga aagccctttt
     8161 tcctgaacga tgcgtgccaa caccggaaaa gcagtaatgc tcatggaaat accgatgaac
     8221 aaggcgaaag aggtaaaatt ggaattggaa ggcgaaaaat ctttgtaaat aacaaatgcc
     8281 agtatcatcc cgaacatgaa agggatgaca atgctggatt ggctgatgat aaacgtgttt
     8341 cggattttat ccttcaattc gctcatattc aggtccatgc ctacagcaaa cataaacaga
     8401 accaagccta tctgactgat aatgtatagg ttgtccaatg aatccggcga aaaaagaaaa
     8461 gcatacgctt cagggaaaat catccccagc aacgacggac ccagcacaat accggccaga
     8521 atttcaccga taaccgttgg ctgctgaatt ttagcaaaaa ctgcactgaa aatgcgtgaa
     8581 acaataagga tggcgataat ctgaagcaaa atcatggcag ccggctccat caggttgtga
     8641 agcgtgaggt ttttgaaaag ttccagtgtg ccctcggttc cttcagctac cgatcgatgc
     8701 agggcaacgt caatcgcatt tccatgtcgg ataaggaaat aagctacgac gccgaagata
     8761 cccagtatca gggcaaagta ccaccacgat ttgtttttgt tcagcatacg gtcaatcgtt
     8821 cagaaacact tcttactgct tacattatgt ggaaattttt agaaatcaat cacaaagata
     8881 gcgaatttta acgcttcatc agttggaaaa caaaccaaat gatgttaaaa aaaaacagaa
     8941 accgacgttt ttgcttactt tattacggtg cattcacgat ggttttttca atatgcaatg
     9001 tctgtgtagg aaatgcaaaa ctgattccgg cttcgttgaa ttttttgaga atttcaaaat
     9061 taatggccga aggggtgtca aaagtgtcgc cggcatcttt cacgtagtaa caatagcgta
     9121 tgtttagcga aaattcaccg aaactttcga acaccgccga tataccttcg ttgctgacag
     9181 cctcattttt taatgcaatg ttgttgagaa tttcaagggc tttttgcatc agttcgggag
     9241 ttgtatcgta ggtaagtccc agattgctca ccacccggag ttgcggtgct gccgaaacat
     9301 tcatgactgc attgtccata attttataat ttggtagcgt tatcagccgt ttgtcgaaat
     9361 tccggatttt tgtgctgcgg ataccgatgt cttccactat gccatcaaat tcaccgattt
     9421 ttacccggtc gccaatgcgg aaagtgccgt ccatcagaat tgtgaaaccg ccgaaaatgt
     9481 tttttaccgt gtcctgagct gccagcgcaa tagcaattcc accgattccg agtgtgccga
     9541 gaatggcttt caggtccagt cccacgttat tgagggctgt tacggtgcct acagtccaga
     9601 tgccgaatac ggcggttttt cgtatcagtg atgtaaggtg cgaatcgaaa tgaatctttt
     9661 gccggatatc tttttcctgt tcactttttt tgataaaata ttcgcggatc agccggctcg
     9721 ccagatgtgc cacaaaccat gtaatgttga gtactgtaag tatttcgtag gttttataaa
     9781 gcgcctcgtc aaacgatttg ctcagttcga gccggttcag ggcagtccag atagctatga
     9841 gaacaatacc cactttcagg ggagtttcca gtgtattcac cagaatatta tcaacctgat
     9901 ttttggtacg ttttgccagc gctttcagat accttcggtt aagaagtgtg ataatccagt
     9961 tgataatcag tgcgaaagcg atgatgccac ccgatatcag ccagttttga agtgtgttgt
    10021 tgtaatatat ttgatcgagc attttatgtt gttatttgcc ttcggtgtta ttttactttg
    10081 ctgttatttg tatttcgcct tattgagtat ttgatataaa gcactttgtt ctttgttctt
    10141 ctaccttccg gcttatatta aaaagaaacc gcatcgcttg gcatccgcca gtcgccccgc
    10201 ggagaaaggt tgatgctacc cacttttggc ccgtcggggg tgcatgagcg tttgaattgc
    10261 tgttggaaaa agcgtttcag gaaaagtttc agccatttat ctatggtttc atcactatat
    10321 ttttctgaaa aagcattttt tgccaggaaa tgaatttttt cgggtgtaaa accgaaacga
    10381 acgaaatagt acaggaagaa atcatgtaat tcgtatggtc caacaatttc ttcggttttt
    10441 tgtgcgatgt ccccgttttt gtcaaatggg agcaattccg gacttacggg agtatccagt
    10501 atatcagtca gaattttttc ggattgatcg tcgagctgat gtgcagccca tcccacaagg
    10561 tagcgtacca gtgttttggg tacaccgctg ttcacaccgt acatggacat gtggtcgccg
    10621 ttgtacgtag accatccgag cgccatttcc gacaaatcgc cggtacccac caccattccg
    10681 ttgtgtttat ttgctaaatc catcaaaatt tgagtccgct cgcgggcttg tacattttca
    10741 tacgtcacat catgcacttc gggattgtgt ccgatatcct taaaatgcag caggcaagct
    10801 tctttaatgt tgatttccat cagggtaatt cccagcgctt tcatcaactc gatggcattg
    10861 ttgtatgttc ggtcggtagt gccgaatccg ggcatcgtca caccaagaat ccgttttcgg
    10921 tcgtagccga gtttatcggc cgttttcacg caaaccagca gcgccagcgt actgtccagt
    10981 ccccctgaaa tgccgataac cagcttgtca aggcgtgtat gctgccagcg ttttgcaagg
    11041 ccggctacct ggatggagaa aatctcatgg caactctcat cgcgcacgct cacaggcgga
    11101 acgaacggat gttgatcaat cttccggttc agtttgaaat ccggattttc aggagcagga
    11161 tcgttttttc tacagtctat ttcaaaataa gcatcttctt cccgttcgtt gaaaaaattt
    11221 gaattgcgca tgcggtctgc acgcaatcgt tctacatcta tctcgctgat agtcagttgt
    11281 ggttcaaagc tgaagcgctc acctttggcc agcactttcc cgttttcaac gatgattgta
    11341 tttcccgaaa aaaccagatc ggtagtggat tctccatgtc cggccgaagt gtacacataa
    11401 ccggcaatac aacgggcgga ttgttgctct acaagttgct ggcgataaat atgcttggca
    11461 atcagctcgt tactggcgga gagattgaac aggatttcgg ctccctgaat ggccaggtac
    11521 gaacttggcg gaacaggcat ccacaggtct tcgcacactt ctatgccgaa ggtgaaatca
    11581 gtggtatgga aataatattt agtgccgaaa ttcgtttcct gaccagcaag ttggatatat
    11641 ccggcagtaa cggttttacc tgaagcaaac catctttttt cgtaaaattc gttgttgttg
    11701 ggcaggttga tttttggaat tacacccaga atttcgcccc ggccgaaaac cactgccgca
    11761 ttgaaaagtt ggttgttttt cctcacgggc attcccacta tgctcagtat gttgagttcg
    11821 gcagtttctt tcaccaattt ctccaaggct ttttcggcat cgtcgagcaa ctgtttctgg
    11881 aaaaacaaat cagcacaagt gtaggctgtg atgcacaatt ccggaaaaca gataatttcc
    11941 acattttgtg ctgctgcatt tacaatcagt tcgttgattt tcaatgcatt gaaagcgcag
    12001 tcggccacac ggagttcggg gatagcagcc gctactctga cgaaaccgaa agccccccta
    12061 aatcccccct tggagggact tgtagttagt ttttccatca ctatctgttc tttattatta
    12121 ttcttgtacc aaaaaattaa attagaaaaa tagttattct tttgaaatta tattgattgt
    12181 atgaataaaa agtccccttc tggtcactga gctatgtcga agtgaggggg ctttttattt
    12241 ttccatatcg ctcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngattcg
    12361 ggtgttttga gcagttcggc cgttttcctg ccaagcataa tgcaggcttg ataattgttt
    12421 tctgtcaatc ccattttttc ctccaccgta aaatctatca tacgccattt catcttttcg
    12481 ccgaattctg ccagcttctt gttggtgata ccggcccagg aataggagcc aaagaatgca
    12541 aaatctcttt tcttcacctg tcgaatttcg atttttgtga gcagcgaatg tatttcggga
    12601 taaagatcat tgcagtaggt ggggcttccg attatcagcc ctttgtactt gaaaatatcg
    12661 cgaagaatga ccgacgcatc cgataccgaa acgttgtgca tcaccacatt gcggatgccg
    12721 ttttgcacca acccctgtgc aacggcttcg gccatacgtt cggtgttgcc atacatagac
    12781 ccgtacacaa cgaccacgcc ttctttggct tcgtagcggc tcagctggtc gtagatgtcc
    12841 actgctttgg atgtttgttc tttccatacc ggtccgtgag tggaacaaat gattttgata
    12901 tccagcgggg tgagtttctg gagcgctttc tggacgggtg caccgtattt gcctacaata
    12961 tttgcgtagt agcgatacat ctcgtcccag tatttgtcca gattcatttc cgaatcaatg
    13021 acacctccat ccagtgttcc gaaacaaccg aatgcatcgc ctgagaaaag cactttttcc
    13081 gattcatcgt aagtcatcat cgtttcgggc cagtgaacca tgggagtgag gtggaactgt
    13141 agtacatgag tgcccagttt cagtatttca ccatcgctca cttccagtgt gttatcgtca
    13201 attccgtagt atccctgcac catttcgagg gtttttttgt tgcccaccag tgttacattc
    13261 ggataatatt ttctcagcag actcagcgaa ccggaatgat caggttccat gtgattgata
    13321 atcaggtaat caatttgtcg gtcgcggagt tgcgattgga ttttatcgat aaaaacatct
    13381 ccgaatgaga tttccaccgt gtcaaccaat gccacctttt cgtcgacaat gagatacgaa
    13441 ttgtaagaca ctccgtaagg taacgggagc atattttcaa atttatgctt tatgcggtcg
    13501 ttgacaccaa cgtaataaat atcttttgca atttccgtgt tcaaatacat agtctttttt
    13561 ataatttctt ccagttaaca ggttatttgt cagaatcaga tctttgaata attaataaca
    13621 tctgatttta atgattggtt ttttatttga aatgcaaaga tacaaattat ttgcagaaag
    13681 ggtaaaaccg gacgctgtgg agtgctaaaa atctggaaaa acctgttagc tgtttttttt
    13741 aatgcgtatt tctaaaaaaa acatattcca caacgttgaa ttttccggtg agcttactaa
    13801 gcgcatcgtc ctacaccccc ttcagagcct gtctcaacca gtcccgattt tatatcggga
    13861 aacttgattc gggagggttg gagagcagat ttttttctta aaagtttacc cgcagggttc
    13921 cgtaaatatt ccggccggga gcgctcacac ccgaagcaaa ctgacggtaa tgccggtcga
    13981 gaatgttttc aattccggca ttcaggttaa tgtgctttgt cacatcatat gcagcacgga
    14041 aatttaacgt gtaccacgcg gcagtacctg cccaggctgt tgcttcgcct ttggcattta
    14101 cggcagtaac agctgtctgg ctgaggttgt cttcgccgct cgggctgtaa tcagccagtt
    14161 tcttggcagc gctgaagcgc ataaaacctt caagtttcag cttgctgaag ttgtaactgg
    14221 ctccaatttt tccatacatt ggcggaatat gatccatcgg aacaacttct ttctgttcgt
    14281 tggtgtattt gccatacgtg gctgttactg aaccattcaa actcagattt ctgacaggat
    14341 tcattgtagc gccgacgtaa taaccataaa ttcgtccgct gttggagttc tgtatggaca
    14401 tcgctttgag gcttgtgcca tcaacgacga cactgtcgct tccgttaaac cgggtcggtt
    14461 gtaccgtgag agcattgtcc agcagcgtat agtaagctcc ggcttcaatt tttcctgtat
    14521 gacccagccg ctgcacgata ttcagttcgg cattgtaggc atattcgggt ttcagtccgg
    14581 tattaggcac tacaaccgtt gtactgcttg tgttgtcgaa aaatttgacc atgtcgtcga
    14641 tgtttggtgc acgaaatccg gtagaaacga ttgcgctgat ttttgttttc gaaggcagat
    14701 tggctaccac cgagaggttg ccgcagggag ctatttcgtt gatttctgct ttatcgaaag
    14761 gaaaaatggc atttccggct gagcggtcat tcttgaattg ggatttcata tacaccgatg
    14821 tcagccgcag accgcctgtc agcgaaaaaa tctgcgacag ggtgtgattg ccctgcacat
    14881 acagcgctgc ggaattccag ctgtttcctc cgtcgggata acgtgtggcg gctggtgagg
    14941 catttgctcc ggtggtgatg tcgatattcg taacggttga ctgcaccagg ttcaatgtct
    15001 cttccagtcc gtaatacagt gttgtccttt cactgatttt tttcatcagg tcgaagttgg
    15061 ccgagaatac gctgacatct tccatctgtg tcttgcggtt cgttttgttc agtttacgcg
    15121 atatgcgatc ctggtctaac gactgaaagg acaggatgaa ttttgcatca tcaaaccagc
    15181 ggttttgttt gttgatatcc agcgtgtaag ctccgaggaa gcgtttttga ggtccgtaat
    15241 tccattcggc atacgttggc agcattgttt ttgttgtttt gtcgtagctg agttccagca
    15301 tgcggtcata gcgtggcaga ttgccaatat tgttggaata ctgcaggttc agcatgtgtg
    15361 aaacatgttc attttgttga tacaaaattt tctcgagtac atcgatttgt ttgtaaccgc
    15421 ttcctgccat aatatatgga ttgggattgg tgctgaccac atctttattg tccacacgcg
    15481 taacgttgta cagatttttg ccgaaatcgc cgaacgttcc gaaatcaccc gattttccaa
    15541 ttcgctgatc gccaaaatcg ctgtaggtga cactggtcag cgatgcgatt ttaccgaagc
    15601 ccaggttata atcgagatga ccggtttttt cgctgtttgc cgacgaatac cgcacatagg
    15661 cattgcccga ttttcggttc agtttcggat ttttagtgta aaaatggata acacctccca
    15721 gcgcgtcaga tccgtacagt gttgacgacg gaccaaaaag cacttcggtt ttgtccagaa
    15781 tagcattgtc gagggtaatc acgtcctgaa catgaccacc acgatagatg gcattgttca
    15841 tgcgaatacc atcaaccacc agtaatatgc ggttggtttc gaagcctctc agtacaggac
    15901 ttccggctcc tgtctggctt ttctgaacaa aaatttgtcc tgtattctgg agcatatcag
    15961 cggtagtttg cgggtttgtg aattcgtttg atttggaact gatcccgata acacgggatg
    16021 gaacattttt gatttcctgt tcccatcggc ttgccgaaac aacaacctcg cggagtgtgt
    16081 cgttataatg tacaggagta gtctgagcaa aggcatatcc ggctatgaga atagccggac
    16141 aaagtaacag tatttttttc ttcatagcag aaactggtga tttcaattga ctgaatataa
    16201 actccgtgat aacggtttta ttaacaggga gttagtgttt ctaaatcaat tgatttttag
    16261 cagataaaat acctgcaatt ttttacattt caatagaaat ttcctgcaaa gatagctatt
    16321 ttcataggtt cattttcagc aaaaaaatca ggatgtggtt atttttgaat tgaataattt
    16381 gtgtagttac ctgtaaatca aatgcatata tattgacgag ttgtaatacc tgttcttttt
    16441 tcttaacttt ttgctcagaa atgtaacaga tgatacgaaa agttacacaa ttctggatgt
    16501 aatcacgaac acgggattag gcccgaagaa taccggttta tagaaaaaag aatttaacac
    16561 gcctgtattt cctgccttcg aaaacgatat tttttaacta cttttgcttt acaaaaaata
    16621 aaagggaaaa tgattgtttg tatagccgaa aagcccagtg tgggtcgtga cattgccgaa
    16681 atactcggag ccagaacgcg taaggacggg tacatggaag gaaacggcta tcaggtgacg
    16741 tggactttcg gacatctgtg tgggctgctc gaacctgccg aatatacacc cgactggaaa
    16801 atatgggcac tctcgcgttt gcccatgata cccaaacctt tcggtattaa agttgttgaa
    16861 aacgaaggca ttcagaagca attccggatc atcgaaaaac tcatttccgg tgccgatgaa
    16921 gtgataaatt gtggtgatgc cgggcaggaa ggcgaactca tacagcgctg ggtgatgcag
    16981 aaagcatcct gtaagtgtcc ggtaaaaagg ctctggattt cgtcgctgac cgaagaagcc
    17041 attcgcgaag gtttcagcaa actgaaagac cagtcggaat atcagcgcct ctacgaagcc
    17101 ggactttcgc gcgccatcgg cgactggctg ctcggcatga atgccacccg cctgtacaca
    17161 ctgaaatacg cccaaaaccg gcaggtactt tccatcggca gggtgcagac gcctacgctt
    17221 gctctaatcg taaaccggca gctcaaaatt gagaatttca aaccggaagc gtattgggaa
    17281 ctgaagacca tctatcgtga aacctccttt tcggctactg caggcaggtt ttcgagtgtt
    17341 gaggaaggac agaattttct gaaaaaggtg gagaatgctc cttttgaggt cactgatatt
    17401 accaccaaaa agggaattga aaatgctccc cgtctgtttg acctcacttc actgcaggtg
    17461 gaatgcaaca agaaattcgg ttattcggcc gatgacacgc tgaaaaccat tcagagcctg
    17521 tatgagaaaa aaataaccac ctatccgcgt gtggatacca cgtacctcag cgacgatatg
    17581 tatccgaaaa ttcccgacat tctccgggga ctgaaggatt acgaactcct gattcagcca
    17641 ctgctggcag ccaaattgcc caaaacaaaa aaggtgttcg acaattcgaa agtgaccgac
    17701 caccacgcca tcatacctac cggagtacag cctgcaaacc tttcggcaga agaaaaaaac
    17761 gtgtatgacc ttgttgcacg aagatttatt gccaattttt atccggaatg caaggtagcc
    17821 accaccaccg tgtcgggcaa agtggaagat gtggaattca aaaccagtgg caaacagata
    17881 ctcggggagg gctggcgggt ggtttttgcc cgtccgacgg ccgaagagaa ggaagaaaac
    17941 ggcgaagagg aagaaaaaac acttccactc tttaccaaag gcgaaagtgg accacacgaa
    18001 ccttcgctga cagagaaatg gacaacaccg cccaaaccat ttaccgaagc aacgctgctc
    18061 cgggctatgg aaacagccgg aaagctggtt gaaaacgacg aactgcgcga tgcgctgaag
    18121 gaaaacggca tcggacgtcc gtccacacgg gctgctatca tcgaaacgct tttcaaacgt
    18181 aactatatac gcaaggaacg aaaaaacctg attgccaccg aaacgggtgt ggaactcatt
    18241 cagattattc aggaagaact gattaaatcg gcagaactga ccgggcagtg ggagagaaaa
    18301 ctacgactga ttgaaaaagg agaatacaaa gcatccgatt ttctggatga actgaaggaa
    18361 atgattatcc gaattgatct gaacgtaaaa agcgacaacc gaaccaaagc acaactgata
    18421 gctgagtcca gactggccga gaatcccgat atgatgatcg gtaccacctg tccggtatgt
    18481 ggtaaaggca gaattatcaa aggtaaaact gcctacggat gttcagaatg gaaaaacggg
    18541 tgtacttttc ggaaaacaat tgtagtagcc ccctaaccct ctgaaggggg gattttggag
    18601 catgctgatt ctggcgaatt actattttaa tgttaggtcg atataaaaat tcatttaatc
    18661 ctttcaaatt caatagtatg aattcccgta gggaaataat atcaatagaa atggnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcttct cgaaactccg tgtgtgggag
    18841 tactttccca tttaaatcgg tttacggctg taactacaaa ggtggttcta cccttgaatt
    18901 tccggtctat ttcgcgcaga tccagacaag tttcggtggt gagtcccact atgttggccg
    18961 ggtcgttcat atcgcccact tctttgcctt tgaagccata caccacataa tacattactt
    19021 cgcagccacc ttcttcatcc acttcatccc agagcaggaa tgcttcctta ccgtctttca
    19081 atattcgcag gttttgcggt tgggctgaag cttcgccttt gatatttttg gggatgggct
    19141 ggagtgccgg gaatttgtag taattgtttt gcaaagtatc ctgcagacct tttggattcc
    19201 ggttgaatgt tttggcactg aaataaactg caccctccac tttcgggtat tgctgattca
    19261 ttttaagctg tcgtgccagt tcattgccgt ttttccaggc aggagtttta ttttcatata
    19321 aacccgatgc ataaagcccg atatacagat tttttccgta agtattgtca ctccaccatt
    19381 tgataagcac ctcatagtca gctacttttt tcccgatttc ccaatacagt tgcggtacta
    19441 tgtaatcgat tgttccctct ttaagccatt tcagaatgtc ggcatagagg tcgtcgtagt
    19501 tcgtaattcc cgcccgtgtg gctgaacctt gcggatccat gttgtcgttt cgccacaccc
    19561 cgaacgggct gactccgaat tgtacccagg gtttcaggtc tttaatggtt ttctgcaatt
    19621 cgctgattac catatccaca ttattgcgtc tccagtcggc ttttttatcg ggagaaaatc
    19681 cgcggggatt ttcgcgaaat gttttttcgt ccggaaaatc gcgtccaccc accggatagg
    19741 gatagaaata atcgtcgaaa tgcagcgcat caatgtcgta tcgcatgatg atgtctgcca
    19801 ccactttatt caggaaatcc cgggtttcat ccagtcccgg atcgaaataa tgttttccgc
    19861 catactccac aaaaagattt ttatttttaa agtaaagatg gtctttatcc agctggttta
    19921 tttgctttgc cgtatttgtt acgcggtaag gattgatcca tccgtgcact tccatgcacc
    19981 gtctgtgggc ttcttcgatg ataaactgaa ggggatcata gtaaggcgac ggacgtttgc
    20041 cttgtttccc gttcaggtaa tacgaccacg gctccaggtc cgaaggataa aaactgtcgg
    20101 ccgtcgggcg aatctgaagt acaatggcgt tgatgccgct tttctgcagt ctgtcaagca
    20161 tttcgcgcat ctcgttgcgt tgctgggttg gcgttaaatc cggattactc ggccagtcga
    20221 tattggcaac ggtagcaatc cacacggcac gcatctcccg tttgagttgt ttctgggccg
    20281 aaagtgtcag aacgaatgaa ataagcagta aaaacgtggt caattttttc atatcagatg
    20341 agaattttgt aaaagttgag caaaaatacg ttttttttct cctttgtgca acctgaatga
    20401 aaatgtctgc atcacgaaaa tcagcggatt aacaaagttt taccgatttt tactactttt
    20461 gtgccatgca acagtttctc aaagatttac atacatcact tcaaaacgtc tattccgaaa
    20521 gtgaaattca cgtgctcggc atgctgatac tcgaaaagtt aaccggtttt tcgcgtacac
    20581 gacttttgac cgataaagag ataatattga acgaaacaca acgagaactc tcaattcact
    20641 ttcttgaaag gctgaaaaaa cacgaaccca ttcaatatat tttaggtgaa accgaatttt
    20701 gcggcctgaa attcaaggtc agtccgtcgg ttctgattcc aagacctgaa accgaagaac
    20761 tggtggagtg ggtaaagccc cttccccaaa gctcccttcc cctttcccga tatgtcggga
    20821 caagcctgga cttcccccac aagggggaag aaaatccccc ctttgggggg tgggggggct
    20881 ttctggatat aggcaccggg agtgggtgta tagcagttgc attaaaaaag aaatttccct
    20941 ctgtcaatgt cacggccatg gatatatccg ctgaagcgtt ggctttggct aaagaaaatg
    21001 cagcactgaa cgaggtgtat attgaattta ttcaggacga tatactgcat ccaaccgcta
    21061 ctaaccgaaa atgggatgta attgtgagca atccgcctta cattccgcat gccggcaaag
    21121 cggaaatggc atccggtgta ctcgattttg agccgcatct ggcgcttttt gtgcctgacg
    21181 atgatccgct attgttttac cgccggatag ccgcttttgc cctggaacat ctcacctcac
    21241 agggccgttt gtatttcgaa atacaccaaa gcctgggccg gcagtgctgc ctgctccttg
    21301 agtcaatggg ctttcgttcg gttgagcttc gcaaagacct gtcaggaaac gacagaatga
    21361 tttcctcaat tcctccagct aaagcaggag gcaattgatg caaaggacag ctaaagcagg
    21421 aggcaattga tgcaaaagac agctaaaaca ggaggcaatt gatgaaaaag acagctaaag
    21481 cagtaggcca ttcatccatt ggattggcct tcaggctatt catttgaaga atatattaaa
    21541 attaggcttc agccaaagaa ttgtacaggg atttggctaa agccattaaa tttgaattga
    21601 tatttatatc ctccagcaaa agcaggaggc aattgatgca aaggacagct aaagaaatca
    21661 agaattcatc aggctcattc ctgttctgaa attcatcatt caacacaaca aacaacattc
    21721 tttttccgtt gtatttatga tagcatcaca catttacaca gtggacttta ttctttattt
    21781 tcacaaccat taatatcaaa aaaaatgaaa acaacccgat tttatgtcat tattgcagca
    21841 tttcttttta cagccgtttc atgtgttgaa aattctcaaa aatacaaagc cgcagttgcc
    21901 gaacgcgatt cgctggctac ggagaaactg atgctggatt ccagttacaa ccagacactt
    21961 gctctcctca acgatattga aaccggattt gccgaaatta cgcaaactga aaagcaaatg
    22021 aaactcaacc tccagagtgt ggaagggccg aaagtaaaca gaaggcagct catagctgca
    22081 caaatgtcgg ctatcaaaca aggtattgaa caaaacaaag cccgaattgc cgaactggaa
    22141 cagttggctt caaagaatgg gaaaataaac accgtgctta ccgaaactgt gaagcgtctg
    22201 cagagccaga tggaagaaaa agggcttcag attaaatccc tgcaggcaga gctcgatcag
    22261 aaaaacatca aaatcaccga gcttactact actgtaaccc aacagagcaa aagcattgcc
    22321 gagcaaagcc gaagcataga agaacagcag caggcgctcg aaaaacagga atcgaccatc
    22381 aaacagcagg ataccgaaat gaacgctgtt tggtactgca ttgcaacacc caaagaactt
    22441 aaagctgcaa aaatcattac agatggcgga cttttccaga gtaaaaaagt actcagcagt
    22501 gaatttgaca attcgatgtt tgcacaggtt gaccagagaa gcgccgtatc catacccact
    22561 tacagcaaaa aagtgaaaat cctctcgcag catcccgtca acagctatac gctcgtgacc
    22621 ggtgaagata aaaacctcac catcgaaatt accgatccgg cccgattctg gagcgtctcg
    22681 aaatacct
//
LOCUS       DAORKP010000014        22234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_90_length_22234_cov_8.942829, whole genome shotgun sequence.
ACCESSION   DAORKP010000014 DAORKP010000000
VERSION     DAORKP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22234
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_22234_cov_8.942829"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLN75_01720"
     CDS             <1..609
                     /locus_tag="PLN75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG02864.1"
                     /translation="FWILLFYFMAPSFPGWATKNWLPTLFSENLGVEMAKAGPTATIS
                     IAIASFLGVLIGGPLSDRWVQKNIRGRMYTSAIGLSLTIPSLILLGFSHTYLGLIGAA
                     TLFGIGYGMFDTNNMPILCQIIPQKYRATAYGIMNMVGVFAGYSVTLMLGSSTDAGSL
                     GADFSKLAIVVLVAVILFLLFLKPKKELTYTSEEIADSGIKH"
     gene            667..1590
                     /locus_tag="PLN75_01725"
     CDS             667..1590
                     /locus_tag="PLN75_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRG02865.1"
                     /translation="MKFEKIKGLIVAPFTPFDKQGELDLSPIAAYGKLLQNNGLIGVF
                     INGSSGEGYMLTVEERMKLAEKWVSTVPEGFQVIVHVGATCIKDSYKLAQHAQEIGAS
                     GIGAMASPFPKAGRVEELVKYCEEIACGAPDLPFYFYHIPVLNGVYLPMLPFLKAAED
                     RIPNLAGIKYTYENLYEYNQCMLYKDGKFDMLHGQDESILNGLIMGGAQGGISGTGSY
                     IGNVLVGIIDEYNKGNIEKARELQNYAQDVINVIAKYRGNIVCGKRIMKFLGLDLGIN
                     RTPFQNITDEEEAIIRKELEAIDFFNRGNKH"
     gene            1814..4063
                     /locus_tag="PLN75_01730"
     CDS             1814..4063
                     /locus_tag="PLN75_01730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRG02866.1"
                     /translation="MTQGEFFERYKYDVKNDKIGIGIYGSVYKAYDTVENRYVALKIS
                     EVKSTEDTEADTEVETGLTGDLPEHVNVLKYESVSTFRQSTGTFDFAVMPFLPSGNLT
                     EILHQNLPDQKSRESIALQLLDGLEFIHNQGVIHRNLKPANILMEVNTTTEGTVFIPK
                     ISDSGLSKTSATGYKHRFTSHSDTETIQYRSPEQLKGEPVRFNTDLWSWAVISYKLLT
                     GNELFTVDTASGELQVILKILEEDVTPKLSGLPENWRIALQDALIKDPSERIKSASGL
                     INILTGDFQEQIIPMDFENEAQEYEEVVPEEYEEVIPEEFEVAIEDEPTAIPPQQKTA
                     QSASTASKKPAVTTVKKARSRKWLYIFPALLIFGVFYYILSGSNVFGHTLSEKKAESI
                     LTNMMEYRSNEQFERTSEVFAEKADKYFSENDVSRSNIIADMEKFAESWSFENTRIID
                     FGRTVENMFHFIMTYDLRDKKTFQITSYKVSGEVGFILENEQYKINYIINRGVANSRN
                     EFSYGNVISRKEQDFSDYMLTYNASVLYLPDVKDHFLLSYIYNGAFTQNPAGFSREQL
                     QKAIQDDYIDFINFSENEKRNDLFDSLTNFSKQIEMSTAFVDADFLCVQVVQNIIKGM
                     SGSGFSLSYRCVDYADGRILALNDVLNTNAVPWSDLIRKSMEREAVTAGNITISAADI
                     SSLPEAPGNFYFDTKKMFFVYGADQIPEAVHFGPVTVSVPLKDLEKYLTTQFKTKILN
                     SRKVNVKRI"
     gene            4138..5133
                     /locus_tag="PLN75_01735"
     CDS             4138..5133
                     /locus_tag="PLN75_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02867.1"
                     /translation="MLIDKIKNLFFGKNNDSDWNTISNQQIVEKIEKVFTEILKRDSV
                     YDRMIFDTDFLILVPPATYERVSLQAPALAKPIISKFYEVIRKQTTKYKDYTPLANYW
                     NLQFAQKEFGVNVPKNEQIIIISKVTAERDWSETLKSEGAAEKISINGKHSKYSNWDI
                     NDEILQKIDILERGKVRVRFNNSLMFEDEKPRPEKATAGNIVAPTPVAASKSSTVNTP
                     QEGLAQIKFREHDKNMNYIMRENLISIGKASGNEKSSLKKLVIHNNESGFEENHLQIR
                     FDPATRIFYIAVFAHTRVNEQSLKISPITSPVWHTLTQNASILCGFYAIKFQALK"
     gene            5237..6319
                     /locus_tag="PLN75_01740"
     CDS             5237..6319
                     /locus_tag="PLN75_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRG02868.1"
                     /translation="MTPELKYAVRTDMGRIRKNNEDNFIEYISPDRSWMVLGAIDGVG
                     GYEGGEIAAQLCKDYVESNIARLDKQILENPQAYLKEILTGANNAIWTERLQKTALSK
                     MSCVASFALMDVRSQLLYFAHVGDTRGYLFRNGELIKFTHDHSVVGYLEDSGSILEQD
                     ALNHPRRNEILKMLGDKLLDVKDAEFIETGSHSFYAGDIVLFCSDGLTDLVNRASISE
                     TLGKTITLDEKVRELIAKANNLGGKDNITAAIAGYPASEQTAGNEPEENVMITSSKNN
                     INSRKKSFKKFLFYFGIFLMGVLTMFLLDRFVFQKETPAKPQFDTDISIDSLRTNPVT
                     PDSLAADSLLNDSIANDSIIPLNHER"
     gene            6309..10427
                     /locus_tag="PLN75_01745"
     CDS             6309..10427
                     /locus_tag="PLN75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRG02869.1"
                     /translation="MKGRIHEFIFLAAVSSVVAFMAVAYYFNIKPELQETEAAYSAGK
                     IINLDGNLKAEKLKELLSKGDYFADTQYENFVAWQLKDKVNAAGGSLSNLGELNKSAF
                     RVKAADMNTSGGELGKSRYLFSATELGIDELPADEKISSLTAKTQVSEQKSGIKISGK
                     VKTGDKKPASNVVVRLTEELPQTVKDSITDSYIKNARIDELIDIDLEKVVKLQSFYAK
                     TDAKGKFAFTNLTKGKNYSVIPLREGKEYGVLKGIAQISHSGRFKFTEKEHRLTLFDQ
                     NSYSRIKKDKILTVRTPEIFKSTYLKSIIFFLLGFWLFFLSLIFRGNRTDPYILPVLM
                     LLSGVGMIVLYSIQDPLRDLDYGSSIGMVVFVTMAVFSVINLVVKEKWILQASRFSLS
                     KELKSRNLLPEIIAGKINPNKQSRGYMWLILSIGLILLLWIFGTGPEGSGVKVNLFGF
                     QISELAKFLMIIFFAKYFAANHDYFREIPNNTYLFRNYFSKMLVMFLVLIAFYLVGIG
                     DQGPAVVLCVTFLIFYSFSKNEFFKMVIAALIYALTLFALAKLTDNNTLIISIYAFTT
                     LVGISVYAYRVRKNESTAFAVLLISSFIILSLFPFDFSERLADRNSMFANIWDNHLHG
                     GDQVAQGVWAISSGGYFGQGLGNGYSNVMPAYHTDMIFESIGEELGILALAAILVLYG
                     ILFYRSMLAARRTGKLFFFYLINGIALVTLVQLAVIVGGSLGLMPLTGISVPFLSKGN
                     VGLAINLGAFLSVIILSQIKGEKHETKFIRENFDNVNAYTLLSFIGIILIFAGTLVYY
                     TLQSDKNMVKPVKTLSKQSEWIYSYNPRIGIFADQLKSGNIYDRNGLLLATDSRDEFI
                     RSAAASEQAFAGMNLFNNQRNKNYKRFYPFSDDLIYWLGNRNNNSVANENTGYVAEFR
                     LLSRLRGFETRPSNVIKATSHVYREFPYLPEETKETNLIKYDYSYFIPFIKAGKNSKL
                     ISKHNERTDLDVKLTLDAKLNQTINSVIRAEEYSKYKVSVVAVKTATGDVLASAQNPA
                     PKLSDIRKISRINPKYYHKLFSASFGYNAFVTDRDFGMYFRSVPGSSVKFIDALAYLR
                     RKGTEGARKTYFISEAERIRQTGYESDPSGNVDMHTAIVRSSNNYFITLMNDQTLHPE
                     LFHLYNTVGINIANIGGFYTRKPENYNNIYFEELWYKRIAPGKELFNSPKLKGTRDRF
                     MKSDYSWIAWGQGPVEATPLQMARLMGSVANGGKLYENNFVLKAPNDTTPYELVEDLE
                     NKEGITSLLASFLKEQSANLSAQTGLTIYGKTGSPERMERYFDKKRGKTRIRKVTDGW
                     YVFYVKNSKFEGSPVAFAVRIQGKGGSSNAKEIAGKILERIKQGYFNGEKAP"
     gene            10747..11721
                     /locus_tag="PLN75_01750"
     CDS             10747..11721
                     /locus_tag="PLN75_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02870.1"
                     /translation="MSKSFLNKLMDKLRYGSEKEFDDDPEQILTTEKPAAEQTKDNDE
                     AVFEEKVDNHSELSAYKNAKKHSVQTPVSNIVELNELSIQAIINAMSAIRYSDVRLSE
                     IHLHVRKDKQDPTSLAVESLFNSEKFMENLKYKLTDAGIHYMDDLKVKINYSSPDVDR
                     YTILVPDLSIRTLTFEEAKKLQKLIITALTGILWEKEIELVPRKKPYFIGRCKMPELD
                     KTGRIINDIAFVGPEENDDPQYEVNRFVSRSIAKIIYNEKTGFFELMRSNLINNKQHV
                     IKIVRIADGVQQISINNTEVGYPLQDDDQIVFNDRVALLVNTAEIDAK"
     gene            11972..13552
                     /locus_tag="PLN75_01755"
     CDS             11972..13552
                     /locus_tag="PLN75_01755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRG02871.1"
                     /translation="MKENIVWDEMFEPLDEIGSGGFSRVLKIRYKPDGKIYALKIMNF
                     DVTDKSREAELNDFYREVEFLKKLSHESIVRIIDDFVIDQKPAILMEYVEGESLAQII
                     RHNRYLPAEEVIEIALQVSAGLMTCHNNADPNLSGLISDTKMPSQAIIHNDIHSKNII
                     KTSRADGTIQYKLIDFGLSFADPEKASKELKIHGMKEFKAPEKWKEEKVSTQSDIYSF
                     GVVLYEMLAGQVPFPISDYDDLTQELRLKNQVLFGKIPPVWSIRKNTIERTDFVTPEE
                     PDFPYWLNNLIMKCLEKEPSKRYRSGKDLNDEIHKGITRQLADEWPDNPEFEDLKNSE
                     IKTDEESTIDISTPNQSRQTTETSEFYQAFMARKEKENERKKTKKRTRIWVVILLALA
                     LVTVGVFLTKNDGKLVKANIEEQIRAYYKADEAAINAESVEMLLNEFEFPVYYYNGMY
                     TRQEFRDFYISKLNAEKKEITLTDITVVKAGNPALVRVHGKLKTYKNANGTKPNYVGE
                     IRDEIIMSDKKIKRIVKQ"
     gene            13856..14209
                     /locus_tag="PLN75_01760"
     CDS             13856..14209
                     /locus_tag="PLN75_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRG02872.1"
                     /translation="MDNVSLFVVVLLAGLTLVVVGLGAALLLSPKSLNFQKGDPYECG
                     LPTHGTSWMQFRVGYYLYAILFLMFDVETIFLLPWSTVVRQLGMSGLISILIFIIILG
                     LGLAYAWRKGILKWT"
     gene            14200..14844
                     /locus_tag="PLN75_01765"
     CDS             14200..14844
                     /locus_tag="PLN75_01765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRG02873.1"
                     /translation="MDLKKVKIKAFPAEDFKDTDTLQAYIDELNANGTNVMVGKLDQM
                     INWGRANSLWPLAFATSCCGIEFMAAAAAHYDLARFGMEVTRNSPRQADLMVVAGTIV
                     HKMGPLLRRVYDQMAEPKYVVAMGACAISGGPFVNSYHVINGAEKIIPVDVYIPGCPP
                     RPESLFYGLLQLQRKVKAEKFFGNKRIVRPYNPATLIDPKTGEALDVKNEFKAP"
     gene            14979..16601
                     /locus_tag="PLN75_01770"
     CDS             14979..16601
                     /locus_tag="PLN75_01770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRG02874.1"
                     /translation="MKDIILKAVPDAVIEEKQKLTVTVEPKQLHPLIKALYSNKELPF
                     DYLINLIGMDWGEKLGVMYLLSSSADMSREVVIITSTDDRQNPLLYSITDMYEAAHLN
                     EREVYAMFGIRFINNPDMRRFLLDDDWNGFPLRKDYDADSRLNPITIVNKEIKDIAPF
                     IMEDADGKLTEGTTRIFDENDYIINIGPQHPSTHGVMHFRTSLDGEIVKKIDVNSGYI
                     HRGIEKLCENMTYPQILHFTDRLDYLSASINRHALCMCIEKAAEIEVPARAEYIRVIM
                     DELNRISSHLIAWSAHCNDLGATTAFIYGVREREHVLDIFDKTCGGRLIVNQNVVGGV
                     MFDIYPDFQKDVKAFIPYMREKLVEYDDFFSHNAIALGRMVKIGNLTREDAISYAVTG
                     PAGRGSGWSCDVRKHKPYSLYDKVDFSEIIRNEGDSYARYMNRLDEIEESLHIIEQLV
                     DNIPEGEILAKVKPIIRLPEGQYFQSVEAGKGEFGVFIESRGDKNPYRVKFRSPSLAA
                     VSAMPLICRDEKLTDFIGIGGSMDYVIPDIDR"
     gene            16829..17956
                     /gene="nuoH"
                     /locus_tag="PLN75_01775"
     CDS             16829..17956
                     /gene="nuoH"
                     /locus_tag="PLN75_01775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRG02875.1"
                     /translation="MNYSQPLELTGIISTLDTFFRNNLTPFWATFIEGALILIAILAA
                     YAIIALALIYIERKITAFFQSRLGPNRLGKYGVIQSIADMVKILIKEIIQINDVDKFL
                     FYAAPFFVIISSVLTFGAIPFGMGLQAVDFKIGIIYVIAASSLGVIGVLLAGWSSNNK
                     YSMIGAMRSGAQFVSYELSAAFSLMTIVVLSGTMQLSEIVENQRYAWNLFNGHIPAMI
                     AFVIYLISGHAETNRGPFDLPEAEGELTAGYHTEYSGLQFGFFYLAEYLNMFIIAAVA
                     TTVFLGGWMPIQIKGLDAFNQVMWYIPSYVWFFAKTGVLIFLSLWVRWSFPRMRIDLL
                     LNLEWKYLLPINLVNILLMVLMVLAGWTLSDWFPGLFATAP"
     gene            18100..18603
                     /locus_tag="PLN75_01780"
     CDS             18100..18603
                     /locus_tag="PLN75_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRG02876.1"
                     /translation="MNPFIKYISGIFTAVKSLLGGMLVTWRELWRKKVTMQYPENRDT
                     LVISDRWRSELTMPHDENNEHACTACGICMINCPNGTINVISKTIETEDGKKKKILDK
                     HIWDYGMCTFCNLCVITCPSDAIIFNNDFEGAMFDRSKLVHQLNKPGSKLREKKPLTP
                     KGEQKAP"
     gene            18709..19248
                     /locus_tag="PLN75_01785"
     CDS             18709..19248
                     /locus_tag="PLN75_01785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRG02877.1"
                     /translation="MAQQIIFYILALAIAVFSILAVTSKRVIRSATYLLFVLLATAGL
                     FLLLNYHYLFAVQVTVYAGGIMVLFIMSIFLTHKPGDQFITRMSWKVRSSAFLAVAGL
                     VLCGYIIVKNVTRVYSYINGNELQMQDLGTTMMGTEKYQYLLPFEVISVLLLACIIGA
                     ILIARKEKSPMNPPQGDMD"
     gene            19370..19669
                     /gene="nuoK"
                     /locus_tag="PLN75_01790"
     CDS             19370..19669
                     /gene="nuoK"
                     /locus_tag="PLN75_01790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRG02878.1"
                     /translation="MLPYLLVSLIMFFAGIYGFFTRKNLILILVSIELILNAVDINFA
                     VFNRYLFPGQSEGLFFALFVIAIAACETAVAIAIIINVYRKFGNAEVDNIQEMKE"
     gene            19799..21721
                     /gene="nuoL"
                     /locus_tag="PLN75_01795"
     CDS             19799..21721
                     /gene="nuoL"
                     /locus_tag="PLN75_01795"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRG02879.1"
                     /translation="MEYTILILLLPLVSFLLIGLTGAALKPKTAGLIGTVTLTLVAFL
                     SYYTAFQYFTSPRVEGVWQTLMPYNFTWMNVGVNLHIDLGILIDPISVMMMIVVSTVS
                     LMVHIYSMGYMKGEKGFQRYYAFLSLFSFSMLGLVLATNIFQMYIFWELVGVSSYLLI
                     GFYYTKPSAVAASKKAFIVTRFADFFFLIGILVLSYLTQTFDFGTLLSGDGSIFNNAL
                     GSTFMGASALTWAMALIFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHAATMVVAGV
                     YLVARLFPIYIGFTPEILHGIAYIGAFTAMFSAVIAIVQTDIKRVLAFSTISQIGFMM
                     VALGVSTSMELNHHEHGGLGYMASMFHLFTHAMFKALLFLGAGSIIHAVHSNDMKDMG
                     GLRKFMPVTHITFLIACLAIAGIPPFSGFVSKDEIMTAAYHFSTGMGVWMTITAGITA
                     FYMFRLYYNIFWGKEYKVHGNHKPHESPLSMTFPLSFLALLTVVGGFIPFGKFVTPDR
                     SEYLIHLDIKIAVISVAVAVTAIALATLLYRRDSKRPAELAGKMSGLYTVAYNKFYID
                     EVYQFITKKIIFNSISRPFAWFDRHVVDGAMNGMARLTGWTSVKTKGFQSGSIQWYAW
                     VFLFGTLLITALAAFL"
     gene            21767..>22234
                     /locus_tag="PLN75_01800"
     CDS             21767..>22234
                     /locus_tag="PLN75_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRG02880.1"
                     /translation="MNFLLIFVLIPVLMTLSLFLTRNRKQVLGIMVTGSIALLAASAY
                     LVVAYVSARNAGASDPMLFTSSMMWYKPLNIGFSLGVDGVSVAMLCLSSVIVLTGTFT
                     SWQIAPLTKEFFLWFCLLSTGVFGFFITTDLFTMFMFYEVALIPMYLLIGIWGS"
BASE COUNT         6615 a         4887 c         4796 g         5936 t
ORIGIN      
        1 ttctggattt tgctgtttta tttcatggct ccgagtttcc ccggatgggc taccaaaaac
       61 tggctgccta cccttttctc cgaaaatctg ggtgttgaaa tggctaaagc cggccccaca
      121 gccaccattt ccatagccat cgcttccttt ctgggcgtgc tgatcggtgg tccgctttcc
      181 gaccgctggg ttcagaaaaa catccgcggc aggatgtaca ccagcgccat aggactttcg
      241 ctcaccattc cttcgctgat tttactcgga ttcagtcata cttacctcgg tttaatcgga
      301 gcagccacac tattcggtat tggctacggg atgttcgata ccaacaacat gccgattctc
      361 tgtcagatta ttccacaaaa ataccgtgcc acagcctacg ggatcatgaa tatggtgggc
      421 gtttttgccg gatattccgt cacactgatg ctcggcagct ccaccgatgc cggaagtctg
      481 ggagccgatt tcagcaagct ggccatcgta gtgcttgttg ccgtaatcct gttcctgctg
      541 ttcctgaagc ccaaaaaaga actgacctac acttcggaag aaattgctga ttcgggaatt
      601 aaacactaaa tcgctgtaat acatgttatt gcttttatta aaaaacatat taaaaaataa
      661 aatattatga aatttgaaaa aattaaagga ctgatcgttg ctccgttcac cccgtttgat
      721 aagcagggtg agctcgacct gagtccgatt gccgcttacg gcaaactgct acaaaacaac
      781 ggattaattg gcgttttcat caatggttca tcgggcgagg gctacatgct gaccgtagaa
      841 gaacggatga aactggccga aaaatgggta tccaccgttc cggagggatt ccaggtaata
      901 gtacatgttg gtgctacctg catcaaggac agctacaagc tggcacagca cgctcaggaa
      961 atcggagcat cgggcattgg cgctatggct tcaccgtttc ccaaagccgg acgggtggaa
     1021 gagttggtaa aatattgtga ggaaatagcc tgcggagccc ccgatttgcc tttctacttc
     1081 tatcacatcc ccgtattgaa cggtgtttac ctgcctatgc ttccatttct gaaagctgca
     1141 gaagaccgga ttccgaatct ggcaggaata aaatacacct acgaaaacct gtatgaatat
     1201 aaccagtgta tgctttacaa agacgggaaa tttgatatgc tccacggcca ggatgaatcc
     1261 attcttaacg gactgattat gggaggcgct cagggcggca tcagcggaac cggaagctat
     1321 atcggaaatg tgctggtagg aattatcgat gaatataaca aaggaaacat cgaaaaagcc
     1381 cgcgaactgc aaaactatgc gcaggacgta atcaacgtga tagcaaaata ccgcggcaac
     1441 attgtttgcg gaaaacgcat catgaaattc ctgggactgg atttgggcat caaccgcact
     1501 cctttccaga acattaccga cgaagaagaa gccattatcc gtaaagaact cgaagccatc
     1561 gacttcttca atcgcggcaa caaacattaa ttgttttttt catctgtaga agattagctg
     1621 catgtgctcc aggcgtgtgc agcttttcgt ttttatagct tcaggctgtt ttctgaatat
     1681 atacaggcaa tcagaaaaaa cgtttgtgat ttctaagcac aaaatccata actctccaat
     1741 catttatttc taaaaaaatc aactatattt gtagctggtt ttcaaaattc tgattaccgg
     1801 catcagcacg attatgacac agggtgaatt tttcgaaaga tacaaatacg atgtaaaaaa
     1861 tgacaaaatt ggcatcggga tttacggatc cgtttataaa gcgtatgata cggttgaaaa
     1921 caggtacgta gccctgaaaa tatctgaagt aaaatcgaca gaggacacgg aagcagacac
     1981 cgaagttgaa acaggcctga ccggtgacct gcccgaacac gtcaacgtgc tgaaatacga
     2041 aagtgtaagc accttccggc agtctaccgg gaccttcgat tttgccgtga tgccgttctt
     2101 accctccgga aatctgacag aaatactcca tcaaaatctt ccggaccaaa aaagcaggga
     2161 atcaattgcg ctccagctcc ttgacggatt ggaatttatt cacaatcagg gcgttattca
     2221 ccgtaatctg aaaccggcca acatcctgat ggaagtaaat accacgaccg aaggcacggt
     2281 attcattccg aaaatatccg attcgggatt atcaaaaaca tccgccaccg gctataaaca
     2341 tcgatttaca agccattctg acaccgaaac aatacaatat cgcagccccg agcaactgaa
     2401 aggggaaccg gtccgtttca ataccgattt atggtcgtgg gccgtaataa gctataagct
     2461 cctcacaggc aatgagttat tcactgtgga tactgcatcg ggcgaactgc aggtcatcct
     2521 gaaaatcctc gaagaagatg taacccccaa actctccggg ctacccgaaa actggcgcat
     2581 tgcgcttcag gatgcattga taaaagatcc ttccgaaaga ataaaatcgg cttccggact
     2641 gataaatata ttaaccggag attttcagga acaaatcatt ccaatggatt ttgaaaacga
     2701 agctcaggaa tacgaagaag ttgtacccga agagtatgaa gaggttatac ccgaagagtt
     2761 cgaagtggcc atagaagatg agcccactgc aataccaccc caacagaaaa ccgcgcaaag
     2821 cgcttccaca gccagcaaaa aacctgctgt aaccaccgta aaaaaagccc gaagcagaaa
     2881 atggttgtat atttttccgg ctttactgat ttttggcgtt ttctattata tcctgtccgg
     2941 gagcaatgtg tttggccata cgctgagtga gaaaaaagca gaatcaatcc ttacaaatat
     3001 gatggaatac cgctcaaacg agcaattcga gcgtacatcg gaagtttttg ccgaaaaagc
     3061 ggacaagtat ttcagcgaaa atgacgtaag ccgcagcaat ataatagctg atatggaaaa
     3121 atttgcagaa agctggtcgt ttgagaacac aagaatcatc gatttcggcc gcacagtcga
     3181 aaatatgttt catttcatca tgacatatga cctgcgcgat aagaaaacgt ttcagattac
     3241 ctcctacaaa gtgagtggcg aagtaggttt catcctggaa aacgaacaat acaaaatcaa
     3301 ttacatcatc aaccgcggcg ttgccaacag ccggaatgaa ttcagctacg gaaacgtcat
     3361 ttcacgaaaa gagcaggatt tcagcgatta tatgctcaca tacaatgctt cggttttgta
     3421 tttgccggac gtaaaagatc attttctgct cagttacatt tacaacggag cattcaccca
     3481 aaatccggcc ggtttcagca gagaacagct tcagaaggcc atccaggacg attatatcga
     3541 ctttatcaac ttttcggaaa acgaaaaaag aaatgacctg tttgattctc tgaccaattt
     3601 cagcaagcag atagaaatga gcacagcatt cgttgatgcc gattttctgt gtgtgcaggt
     3661 tgtgcaaaac atcataaaag ggatgagcgg ttccggtttt tcgctttcgt accggtgtgt
     3721 ggattacgca gatggcagaa ttcttgctct gaatgatgtc ttgaatacga atgcagttcc
     3781 ctggtcagat ctgatcagga aaagtatgga gcgcgaagcc gtcacagcag gcaacataac
     3841 catatctgca gccgatattt cctcgttgcc cgaggcaccc gggaatttct atttcgacac
     3901 caaaaaaatg ttttttgtat atggcgccga tcagattcct gaagcagttc atttcggacc
     3961 tgttacagtt tcggtgccgt tgaaagacct ggagaaatac ctgacaaccc aattcaaaac
     4021 aaaaatactg aatagcagga aagtgaatgt aaagagaata taaatcggta taacaatgac
     4081 ttaatcttac ttaatttccg ttttggcagt tgagtaaagc aaataaaaca ggaagatatg
     4141 ttaatagaca aaattaaaaa cctttttttc ggcaaaaaca acgacagcga ctggaacacc
     4201 atttccaatc agcagattgt ggaaaagatt gaaaaagtat tcaccgaaat actcaaacgc
     4261 gacagcgtat atgacaggat gatattcgat accgactttc tgatactcgt cccaccggct
     4321 acttatgagc gtgtatccct gcaggcaccc gcactggcaa aacccatcat cagtaagttt
     4381 tacgaagtaa tccgcaaaca gacaaccaaa tacaaggatt acacgccgct tgccaattac
     4441 tggaatctgc agttcgcgca gaaagaattc ggcgtaaatg tacctaagaa tgagcaaatt
     4501 atcatcataa gcaaagtaac agccgagcgc gactggagcg aaacactgaa atcggaagga
     4561 gctgccgaaa aaatatccat caacggaaaa cattccaaat attccaactg ggacatcaac
     4621 gatgaaattt tgcaaaaaat tgatatcctc gaacggggaa aagtgagggt gagattcaac
     4681 aatagcctga tgttcgaaga tgaaaaacca cggccggaaa aagcaacggc cggaaatatt
     4741 gtcgcaccca ctccggtggc agcttccaaa tcttcaactg taaatacacc tcaggaaggt
     4801 ctggcgcaaa tcaaatttcg cgagcacgac aaaaacatga attacatcat gcgcgaaaac
     4861 ctgatttcca tcggaaaagc atcaggcaat gaaaaatcat cattgaaaaa gctcgtcatt
     4921 cacaacaacg aaagcggttt tgaggaaaat cacctgcaga ttcgcttcga tcccgcaacg
     4981 cggatattct acatcgcagt gtttgctcat acgcgggtaa acgaacaaag cttaaagata
     5041 agccccataa ccagtccggt gtggcatacg cttacgcaaa atgcctccat tctatgcgga
     5101 ttttatgcta tcaaatttca ggccctcaag tagaaaaatg acatcaagac cggaaaactg
     5161 atagaaatga taatgatagt aacatattga tacggtgaaa tggtgacttt gtgatattaa
     5221 acgattatta taaattatga cacccgaact taaatacgct gtccgcaccg acatgggccg
     5281 gatccggaaa aacaacgaag ataattttat cgaatacatt tcgcccgaca gaagctggat
     5341 ggtgctgggt gctatcgacg gagtgggcgg atacgaaggt ggtgaaattg ctgcacagct
     5401 atgcaaagac tatgtggaaa gtaacattgc caggttggac aagcagattc ttgaaaatcc
     5461 gcaagcctac ctgaaagaaa ttctcaccgg agccaacaat gccatctgga ccgaacgatt
     5521 acaaaaaact gcgctgagca aaatgagctg tgtggcctcg tttgcactga tggacgtacg
     5581 cagtcagctg ctttactttg cccatgtggg cgatacacgg ggttatttgt ttcgaaacgg
     5641 cgaactgatc aagtttacac acgaccattc cgtagtaggt tatctggaag attcaggctc
     5701 catactcgaa caggatgccc ttaatcaccc ccgacggaat gaaattctga aaatgctggg
     5761 cgataaactg ctggatgtga aagatgcgga atttatcgaa accggctcac acagctttta
     5821 tgcgggcgat atcgtgcttt tttgcagtga cggtctcacc gatctggtaa acagagcttc
     5881 catatccgaa acactgggca agaccattac gctggacgaa aaagtgaggg aactgatagc
     5941 caaagcaaac aacctgggag gcaaagacaa cattacagct gccattgcag gttatcctgc
     6001 ctccgagcaa acagccggca acgaaccgga agaaaatgtg atgatcactt catcgaaaaa
     6061 taatattaat tcacgaaaaa aatcatttaa aaaattcctt ttttatttcg gaatcttcct
     6121 gatgggtgta ctcaccatgt ttttgctgga caggtttgtc tttcaaaagg aaactccggc
     6181 aaaacctcag tttgataccg acatcagcat cgattcgctg cgcaccaacc cggttacgcc
     6241 cgacagcctt gctgccgact cactactaaa cgactccatt gcaaacgact ccataatacc
     6301 cctgaaccat gaaaggtaga atccacgaat ttatatttct ggcagcggta agcagcgtag
     6361 ttgcattcat ggctgtggct tattatttca acattaaacc ggaattacag gagactgaag
     6421 cggcgtattc tgccggaaaa atcatcaatt tagacggcaa tttaaaggcc gaaaaactga
     6481 aagaactgct ctcgaaaggc gattattttg ccgatacgca gtatgaaaat tttgtagcgt
     6541 ggcagctgaa agacaaagtg aatgcagcag gtggcagcct gagcaacctg ggtgaactca
     6601 ataaatcggc attcagggta aaagctgccg acatgaacac ttccggcggc gaactgggta
     6661 aatcaagata cctcttttct gccacagaac tgggaattga cgaactgcct gcggatgaaa
     6721 aaatcagctc cctgactgca aaaactcagg taagcgaaca aaaatcaggg ataaaaattt
     6781 ccgggaaagt aaaaaccggg gataaaaagc ctgcttcaaa cgtggtggta cgactcaccg
     6841 aagaactgcc tcaaacagta aaagacagca ttacagacag ttacatcaaa aatgcccgga
     6901 tagacgaact gattgatatc gacctggaga aagtggtgaa actccagagc ttctacgcca
     6961 aaaccgatgc gaaaggcaaa tttgcattta ccaacctcac aaagggtaaa aactactcgg
     7021 taattccgct tcgggaagga aaagaatacg gcgtgctcaa aggaatagcc caaataagtc
     7081 attccggcag atttaagttt accgaaaaag aacaccggct cacacttttt gaccaaaaca
     7141 gttattcacg aattaaaaaa gacaaaatcc tgactgttcg cacacccgaa atattcaaaa
     7201 gtacctacct gaaatcaatc attttctttc tgctgggttt ctggttgttc tttttatcgc
     7261 ttattttcag aggaaatcgt accgatccgt atattctgcc tgtgctgatg ctgctgagcg
     7321 gtgtgggaat gattgtactt tattccattc aggatccgct gcgcgatctg gattacggtt
     7381 cgtccattgg gatggtggtt ttcgtcacga tggctgtttt cagtgttatc aatctggttg
     7441 taaaggaaaa atggatcctt caggcttcga ggttcagcct gagcaaagag ctgaaatcca
     7501 gaaatttact ccccgaaatt attgccggaa aaatcaatcc gaacaaacaa tcgcgcggct
     7561 atatgtggct catattatcc atcggactca ttttactgct gtggattttc ggcacaggac
     7621 ccgaaggaag cggtgtgaag gtgaatttat tcggctttca gatcagcgaa ctggctaaat
     7681 ttctgatgat aattttcttt gccaaatatt tcgctgccaa tcacgattac ttccgcgaaa
     7741 taccgaacaa cacgtatctt ttccgcaatt atttcagcaa gatgctggtc atgttcctgg
     7801 tacttatcgc attttatctc gtcggaatag gcgatcaggg accggctgtg gtgctttgcg
     7861 ttacctttct gattttctat tcgttttcca aaaacgaatt tttcaaaatg gtaattgcag
     7921 cgctcatcta tgcactcacg ttgtttgccc tggccaaact gactgataac aatacgctca
     7981 ttatcagcat ttatgctttt acgacactcg tcggtatcag cgtatatgcc tatcgggtca
     8041 ggaaaaacga gagtacggct ttcgccgttt tgcttatttc gtcgtttatc attctgtcgc
     8101 ttttcccgtt tgatttctct gagcggcttg ccgaccgcaa cagtatgttc gccaacattt
     8161 gggacaatca cctgcatggc ggcgaccagg tggctcaggg agtctgggca atcagttccg
     8221 gagggtattt cgggcagggt ctgggtaatg gttactccaa cgtaatgccc gcctaccaca
     8281 ccgacatgat tttcgaaagc atcggtgaag agcttggaat actcgctctg gcagctattc
     8341 tggtacttta cggaattttg ttctaccgct ccatgctcgc agcacgccgc accggaaagc
     8401 tcttcttttt ctatctgatc aacggaattg cgctcgtcac gctggtacaa cttgccgtca
     8461 ttgtgggagg atcgcttgga ctgatgccgc ttacaggcat ttcagtaccg tttttgagta
     8521 aaggaaatgt aggtttggcc atcaatctgg gagcatttct ttcggtaatc attctttcac
     8581 aaatcaaggg agaaaagcat gaaaccaaat ttatccggga gaattttgat aatgtaaatg
     8641 cctacacatt gctttcattc atcggaataa tcctgatatt tgccggaacg ctcgtatatt
     8701 acactcttca gtccgataag aacatggtga aacccgtaaa aacactttcg aagcaaagtg
     8761 aatggatcta cagctacaac ccgcgtatcg gcatttttgc cgaccagctc aaatcgggaa
     8821 atatctacga ccgcaacggg ctgctgcttg ctaccgacag ccgcgatgaa tttatccggt
     8881 cagcagcggc ttccgaacag gcttttgccg gcatgaattt attcaacaat cagcgaaata
     8941 aaaattacaa acgattttat cccttcagcg atgacctgat atactggctt ggcaaccgca
     9001 acaacaacag tgtggcgaac gaaaacacag gctatgtggc agaattcagg ttgctatcca
     9061 ggttgcgtgg ttttgaaacc agaccatcca atgtaatcaa agccacatcg cacgtttatc
     9121 gggagtttcc ttatcttccc gaagaaacaa aggaaacaaa tctgattaaa tatgattaca
     9181 gctatttcat accatttatt aaagcaggaa aaaacagcaa actgataagc aaacacaacg
     9241 aacgcaccga cctggatgtg aaactgacac tggatgccaa attgaatcaa actatcaaca
     9301 gtgtgatcag ggccgaagaa tacagcaaat acaaagtttc ggtagtggct gtcaaaacag
     9361 ctacgggcga tgtgctggct tcggctcaga atccggctcc caaactgagc gatatccgga
     9421 aaatttcgcg catcaatcca aagtactacc acaaactttt cagtgcaagt ttcggataca
     9481 atgcttttgt caccgacagg gattttggta tgtatttcag atcggttccg ggctcttcgg
     9541 tcaaatttat cgatgcactg gcatacctac gccgcaaggg aaccgaaggc gcccgcaaaa
     9601 cctatttcat cagcgaggcg gaacgcatca ggcagaccgg ttacgaaagc gatccaagcg
     9661 gcaatgtgga tatgcacacc gccatagttc gctccagcaa caactatttc atcaccctga
     9721 tgaacgacca gacgcttcat cccgaacttt ttcatcttta taatacagta ggaatcaaca
     9781 ttgctaatat cggcggattt tatacccgaa aacctgaaaa ttacaataac atctatttcg
     9841 aagaactttg gtacaagcgc attgctcccg gaaaagaatt attcaacagc cctaaactga
     9901 aaggcacacg cgaccggttt atgaagtcgg attactcctg gatagcctgg gggcagggac
     9961 ctgtggaagc tacaccgctt cagatggcgc gcttgatggg ttcggtagcc aacggtggaa
    10021 aactttatga aaataacttc gtactgaaag caccaaacga caccacaccc tacgagctgg
    10081 tagaagatct ggaaaacaag gaaggaatta cctccttact tgcctcgttc ctcaaagaac
    10141 aatcggcaaa cttatcggct caaacagggc tcacaattta cggaaaaaca ggttctcccg
    10201 aacggatgga acgttatttt gataaaaaaa ggggcaaaac ccgcataagg aaagtgaccg
    10261 acggatggta tgtattctat gtgaaaaatt caaaatttga aggatcacct gtggcttttg
    10321 cagtgcgtat tcagggcaaa ggaggctcgt caaacgcgaa ggaaattgcc ggaaaaatac
    10381 tggaaagaat taaacaaggg tattttaatg gagaaaaagc tccttgaatt tccgttacag
    10441 atttttaatt ctgaacgtct cgacttttaa aacatagata acaatagtct tattttattt
    10501 aattttcgtg ctttttaaaa tgatttcgat aacaatagtt taaaaatatc gaagatattt
    10561 ttttctatgt ttccgaaaat aagataataa gtgtaaatca ttctatgttt tctattgttt
    10621 taaatgtttt ctattgtttt aagaaaagtc aagatgactt ctctttgaat ttatgccata
    10681 taagcaaatt catccataaa atatcgggac ataaaaagtg taaaatacaa tttaaatcaa
    10741 aataaaatga gcaaatcatt cttaaataaa ctgatggaca aacttcgcta cggatctgaa
    10801 aaagaattcg atgacgaccc tgaacaaatc cttactaccg aaaaaccggc tgctgaacaa
    10861 acaaaagata atgatgaggc tgtttttgaa gagaaagttg ataatcacag cgaattatct
    10921 gcgtataaaa atgcaaaaaa acattccgtt caaaccccgg tttccaacat cgtggaactg
    10981 aacgaactga gtattcaggc cattatcaat gccatgtcgg ctatcaggta cagcgacgta
    11041 aggctgtcgg aaatacatct ccacgtccgc aaggacaagc aggatcccac ttcactggca
    11101 gttgaaagtt tgttcaacag tgaaaagttt atggaaaacc tgaagtacaa actaaccgat
    11161 gccggaattc actacatgga cgacctgaag gtgaaaataa attattcctc acccgatgtg
    11221 gaccgctaca ccatacttgt acccgatctg agtatccgga cgctcacttt tgaagaagcc
    11281 aaaaaacttc agaaactgat tatcactgcc ctgacgggta ttttgtggga aaaagaaatt
    11341 gagttggtgc cacgcaagaa gccctacttc atcggcagat gcaaaatgcc cgaactggat
    11401 aaaaccggcc ggatcatcaa cgacatcgcg tttgtgggtc cggaagagaa tgacgacccg
    11461 caatacgaag tgaaccggtt tgtgagccgc tccattgcca agattatata caacgaaaaa
    11521 acgggcttct ttgagttgat gcgatccaat ctgataaaca acaaacagca tgtcatcaaa
    11581 attgtacgta tcgccgacgg agttcagcaa atttcaatca ataacaccga agtgggctat
    11641 ccgcttcagg acgacgacca gatagtattc aacgaccggg tggcgctgct ggtcaataca
    11701 gcagaaatag atgcgaaata acaactcagc ccccctgaat accgcatagg tgactttcag
    11761 aacaagtttt ctgcatttat ttttttactt aaaagagaat tctttataca aaaaaaacag
    11821 gcttattctg ttaaccttta atagagttct taatatagaa atgactactt aacagaaaaa
    11881 acaattcgta taacctgcct gcggcaggca gggtcgctct aattcgtatt aattcgttta
    11941 attcgctcta attcttgcaa ttcgtatatc aatgaaagaa aatatcgttt gggatgaaat
    12001 gtttgaaccg ctcgatgaaa ttggatcggg cggtttttcg cgtgttttga aaatcaggta
    12061 taagcccgac gggaaaatct atgcactgaa aatcatgaat tttgatgtca ccgataaatc
    12121 cagggaagcc gaattgaacg atttctaccg cgaagtggaa tttctgaaaa aactaagcca
    12181 tgaaagcatc gttcgtatca tcgacgattt tgtgatagat caaaaaccgg ccatcctgat
    12241 ggaatatgtg gaaggtgaat cattggcaca gattatcaga cacaacaggt acctcccggc
    12301 agaagaagtc atcgaaattg cactgcaggt ttcggccgga ctcatgacat gccacaacaa
    12361 cgcagatccg aatctgtcag gtttgatttc cgatacaaaa atgcccagcc aggctattat
    12421 tcacaacgat attcattcaa aaaatatcat caaaacttcg cgtgctgacg gcaccattca
    12481 atataaactc attgatttcg gactttcgtt tgccgatccg gaaaaagcgt cgaaagagtt
    12541 gaaaatacac ggaatgaaag aattcaaagc gcccgaaaag tggaaagagg aaaaagtgag
    12601 tacccaatcg gatatttaca gtttcggagt ggtgctttac gaaatgctgg caggccaggt
    12661 accctttccc attagcgact acgatgatct gacacaggaa ctgcggttga aaaatcaggt
    12721 tttgttcgga aaaattccac ctgtctggag catccggaaa aacaccatcg aaagaaccga
    12781 tttcgtaact cccgaggaac ctgattttcc ttactggctt aacaatttga taatgaaatg
    12841 tctggaaaaa gaaccttcca aacggtatcg ttcaggaaag gatctgaacg atgaaattca
    12901 caagggaata acaaggcaac ttgcagatga atggcctgat aatccggaat ttgaagattt
    12961 aaaaaattct gaaattaaaa ccgacgaaga atcaaccatt gacatttcaa cgccaaatca
    13021 atccaggcaa actactgaaa catccgaatt ttatcaggca tttatggcca gaaaagagaa
    13081 agaaaatgag cgaaaaaaaa caaaaaaaag aacccggata tgggtggtga tattgcttgc
    13141 attagcttta gtaacagttg gtgtattttt gaccaaaaac gacggaaaat tggtcaaagc
    13201 caacatcgaa gagcaaatcc gtgcatacta caaagccgac gaagccgcta tcaatgccga
    13261 aagcgtggaa atgctgctga acgagtttga atttccggta tattattaca acggtatgta
    13321 cacccgccag gaattcaggg atttctatat cagcaagctg aacgcggaaa agaaagaaat
    13381 cacattgacg gacattactg tggtaaaagc aggaaatccg gctttggtac gtgtgcacgg
    13441 taaactgaaa acctataaaa atgcaaacgg cacaaaacca aattacgtag gcgaaatcag
    13501 agatgaaatt ataatgtctg acaaaaaaat aaaacggata gtaaaacagt aacttttagt
    13561 tattcacgga agccaaaact ccggcaaaaa aacgtatatt ctgtaaaata acaaaataat
    13621 gtcagaaact ttcttgtaga aatatcaatt ttgtaatctc gacattgcaa aataaacaaa
    13681 aattaatcgc attaatgaaa atattacaaa actattgtgt acctttgcac agtttttaat
    13741 aagacattcg ttcgttcagt cagactcact cgttcaaatg gcattcaaaa acagccattt
    13801 aagagttctt tttttacaga cattttattc attttaaaca tttaactata tttctatgga
    13861 taatgtatca cttttcgtgg tagtccttct ggctggttta acattggttg tcgtcggatt
    13921 aggcgccgca ctgttactct cacccaaatc cctcaatttt caaaaaggcg atccgtatga
    13981 atgcggactt cccactcacg gcacatcctg gatgcagttc agggtgggtt attacctgta
    14041 tgccatactg tttctgatgt tcgacgtcga aaccatcttc ctgttacctt ggtcaacggt
    14101 ggttcgtcag ctgggaatgt cgggattgat tagtattctt atttttatca ttattctcgg
    14161 cctgggactt gcctacgcct ggaggaaagg aattttaaaa tggacttaaa aaaagtaaag
    14221 ataaaagcat ttcctgcaga agatttcaaa gacacggata cgctgcaggc atacatcgac
    14281 gagctgaacg ccaacggcac caacgtgatg gtaggaaagc tcgaccagat gatcaattgg
    14341 ggacgcgcca actcgctttg gccgctggca tttgccacca gctgctgcgg aattgaattt
    14401 atggccgctg cagccgctca ttatgactta gcacgttttg gtatggaagt tacccgcaac
    14461 agcccgcgcc aggccgacct gatggtggtg gctggaacca ttgttcacaa aatgggaccg
    14521 ctgctccgcc gtgtgtacga ccagatggcc gaaccaaaat atgtggttgc gatgggagcc
    14581 tgtgccattt cgggtggtcc gtttgtcaat tcgtaccacg tcatcaacgg tgccgaaaag
    14641 atcattccgg tagatgtgta cattcccgga tgtccgcctc gtcccgaatc gcttttctac
    14701 ggcctgcttc agctgcagcg caaggtgaaa gctgaaaaat tctttggtaa caagcgcatt
    14761 gtaagacctt acaatccggc tacgcttatc gatccgaaaa caggagaagc tttggatgtg
    14821 aaaaacgaat tcaaagcccc ctaaccccct gaagagggaa tgataatgcc acaattcagc
    14881 attatcacat ttttgtattt ttattattat tattattcac taaagttgac atacgctctc
    14941 aaaattaccc cttcaggggg ttagggggca gataatttat gaaagacata atactaaaag
    15001 ctgttcccga tgcggtgatt gaggaaaaac aaaagctcac tgtaaccgtt gaacccaaac
    15061 aacttcaccc tttaatcaaa gcgctttatt ccaacaaaga attgccattt gattacctga
    15121 taaaccttat cggaatggat tggggcgaaa aactgggtgt gatgtacctg ctttcatcct
    15181 ctgcagatat gagccgcgaa gtggtcataa tcacctcgac cgacgaccgc caaaatccac
    15241 tgctgtattc cattaccgat atgtacgaag ccgctcatct caacgagcgc gaagtatatg
    15301 ccatgttcgg aatccgtttt atcaacaatc cggatatgcg gcgctttctg ctggacgacg
    15361 actggaatgg gtttccgctc cgcaaagatt acgatgcaga ctccagactc aatccaatca
    15421 ccattgtcaa taaagaaatc aaggatattg caccttttat catggaagat gccgacggca
    15481 aactgaccga aggcacaacc cgtatttttg atgaaaacga ttacatcatc aacataggcc
    15541 cgcaacaccc ttccacgcac ggagtaatgc acttccgcac ctcgctcgac ggtgaaatcg
    15601 taaagaaaat tgatgtcaac agcggttaca ttcaccgtgg catcgaaaaa ctttgcgaaa
    15661 acatgacata cccgcagatt ctgcacttta ccgaccggct ggattacctc tcggcaagca
    15721 tcaaccgcca tgcactttgc atgtgtatcg aaaaggctgc cgaaatagaa gtacctgccc
    15781 gcgccgaata cattcgggtc atcatggatg aactgaaccg aatcagttcg cacctcattg
    15841 cctggtcggc tcactgcaac gacctgggag caactaccgc atttatttat ggagtacgcg
    15901 aacgggaaca tgtacttgat attttcgaca aaacatgcgg tggacgtctt attgtcaatc
    15961 agaatgtggt gggtggcgtg atgtttgata tttatcccga cttccagaaa gatgtgaagg
    16021 ctttcatccc ctacatgcgc gaaaaacttg tggaatacga tgacttcttt tcgcacaatg
    16081 ccattgcact tggacgtatg gttaaaattg gaaatcttac ccgcgaagat gctatcagtt
    16141 acgcagttac aggtcctgcc ggcagaggtt cgggctggtc gtgcgacgtg cgcaagcaca
    16201 aaccatattc actttatgac aaagtagatt tcagtgaaat cattcgaaac gaaggcgact
    16261 cgtatgcccg ctacatgaac aggctggatg aaattgaaga atcgctgcat attatcgaac
    16321 agcttgtgga caacattccc gaaggcgaaa ttctggcaaa agtaaaacca atcatccggc
    16381 tgcccgaagg tcagtatttt cagagtgtcg aagccggtaa aggtgagttt ggcgtattta
    16441 tagagagccg tggcgacaag aatccgtatc gtgtgaaatt ccgctctcca tcactggcag
    16501 ccgtatcggc catgccgttg atttgcaggg atgaaaaact gaccgacttt atcggtatag
    16561 gcggttcgat ggactacgtg attccggata ttgacagata gccccctaaa tcacccgaaa
    16621 ggggacttta cggtgtactg caaatagatt ttagataata aaaaactaac attgtatgaa
    16681 ccataattcc attttccgga attagttcaa ttgttcagat ttcaattatt tacttttttc
    16741 aaaaaatttc agaataaaga gagatttcac tattaattaa atttttaact cacaataaat
    16801 tctcccttcg gggggattaa gggggcttat gaattactcg caaccattag aactgacagg
    16861 gattatttct actctcgata cctttttcag aaataatctg acaccattct gggctacttt
    16921 tatcgaagga gcgctcatac ttattgccat actggccgcc tatgccatta tagcgctggc
    16981 tctgatctat atcgagcgca aaatcaccgc attctttcag tcgcggctcg gtccgaaccg
    17041 gcttggcaaa tacggcgtaa tccagagtat tgccgacatg gtaaaaattc tgattaaaga
    17101 gattatccag atcaatgatg tagataaatt tctgttttat gcagctccgt tttttgtaat
    17161 catttcatcg gtgcttacat tcggtgctat tcctttcggt atgggattac aggcagtgga
    17221 tttcaaaatc ggtataatat atgtcattgc tgcctcgtcg ctgggcgtga ttggtgtgtt
    17281 gctggcagga tggtcgagca acaataaata ctccatgatc ggagctatgc gaagcggtgc
    17341 gcagtttgta agctatgagc tttcggctgc attttcactg atgacaatcg tagtgctgtc
    17401 aggcaccatg cagctttcag aaatagttga aaatcagcgt tatgcctgga acctgttcaa
    17461 cggacacatc ccggcaatga tagcttttgt gatttacctg atctccggac atgccgagac
    17521 caaccgcggt ccgtttgacc ttccggaggc tgaaggcgag ctgactgccg gataccacac
    17581 ggaatattcc ggtctccagt ttggtttttt ctacctggcc gaatacctga acatgttcat
    17641 tatagcagca gtagccacca ccgtattctt aggcggctgg atgcccattc agataaaagg
    17701 gcttgacgca ttcaaccagg taatgtggta tattccttct tacgtttggt tctttgccaa
    17761 aacgggtgta ctcatattcc tgagtctctg ggttcggtgg tcattcccgc gtatgcgtat
    17821 cgacctgctc ctcaacctgg agtggaaata cctgctgcct atcaatctgg tgaatatctt
    17881 actcatggtg ctgatggtgc ttgccggctg gacattgtct gactggttcc cggggctgtt
    17941 tgctacagcc ccttaaattc cccgaaggag gactttaaga gcgcattgct caaattccac
    18001 tttatcagaa agattttttt ttattttatt agatacaaac aacttcaaaa taacccaaaa
    18061 tattccccct tcggggttta gggggctgac attaataata tgaatccttt tataaaatac
    18121 atttctggaa tttttactgc cgtgaaatcg cttcttggcg gtatgttggt aacctggaga
    18181 gaactctggc gtaaaaaggt aaccatgcag tatcccgaaa acagggatac actggtaata
    18241 tccgaccgtt ggcgctcgga actcactatg ccgcacgacg agaacaacga acacgcctgt
    18301 acggcctgcg gtatctgtat gattaactgt ccgaacggca ccatcaacgt gatttcaaaa
    18361 accatcgaaa cggaagatgg aaagaaaaag aaaatactgg ataagcacat ctgggattac
    18421 gggatgtgta ctttctgcaa tctttgtgtg ataacctgcc cttcagatgc cattatcttc
    18481 aacaatgatt tcgaaggagc catgttcgac aggagtaaac tggttcatca gctgaataaa
    18541 ccgggatcaa agttgagaga gaagaaaccc ctaaccccta aaggggaaca aaaggccccc
    18601 taaatcccca aaggttcata aaaaacaaat aaatcaatca aacttggtaa tttcaaattt
    18661 ttcaaaactc aaaacccggt acttgaaact cgaaacttta aaacatatat ggcacaacaa
    18721 attatatttt atattttagc gctggcaatc gccgtatttt ccatactggc agttacgagc
    18781 aaacgggtaa tccgctcggc aacctatctg ttgtttgttt tgctggctac cgcaggattg
    18841 tttcttttac tcaattatca ttatcttttt gctgttcagg tcactgttta tgcaggcggc
    18901 atcatggttc tgttcatcat gtcgatattt cttactcata aaccgggcga tcagtttata
    18961 acccgaatga gttggaaggt cagatcgtcg gcattcctgg cagtagcagg acttgtcctg
    19021 tgcggataca tcatcgtaaa gaatgtgacc cgcgtttaca gctatatcaa cgggaatgaa
    19081 ctgcagatgc aggatttagg tactacaatg atgggtaccg aaaaatatca gtacctgctt
    19141 ccgttcgaag taatcagtgt gctgctgctt gcctgtatta ttggagctat tctgattgcc
    19201 agaaaagaaa aaagccccat gaatcccccg cagggggata tggattaaat gcagaatctg
    19261 tattttccta attttcaatc atgcactatt cgcgtaattc gtttataatt cgtaaaattt
    19321 gtatcctatg ggagaactgt taataaatac atccggagca ccggtaccaa tgttacctta
    19381 cctgcttgta agtctgataa tgttttttgc aggcatatat ggtttcttta cccgcaagaa
    19441 tcttattttg atacttgtat ctattgagtt gatattaaat gcagttgata tcaactttgc
    19501 cgttttcaat cggtatcttt ttcctggaca gtccgaggga ttgttttttg ccctgtttgt
    19561 aatagccatt gctgcctgtg aaaccgctgt agccattgcc attatcatca acgtgtaccg
    19621 caagtttgga aacgccgaag tggacaacat tcaggagatg aaagagtaaa agccccctaa
    19681 atcccccaca aggtggactt aatacaatac aaatgatcaa tctatataat aaataacgct
    19741 aacctcgctc tttctcttcc ccttcggggg agttggaggg ggctttttat atcactttat
    19801 ggaatatacc attttaattc ttttgcttcc gctggtgagt tttctgctta tcggattgac
    19861 aggagcagca ttaaaaccaa aaacagccgg acttatcggg acggtaacat tgacacttgt
    19921 tgcttttctg tcctattaca cggcttttca gtactttaca tctcccagag tggaaggcgt
    19981 atggcagact ctgatgcctt ataacttcac gtggatgaat gtcggggtca atctgcatat
    20041 cgatttgggc attttgattg atccgatttc cgtgatgatg atgatagtag tctctaccgt
    20101 ttcgctgatg gtgcatattt acagtatggg ctatatgaaa ggcgaaaaag gttttcagcg
    20161 ttactatgca tttctttcgc tgttttcatt ctccatgctc ggactggtgc tggctaccaa
    20221 cattttccag atgtatattt tctgggagct ggtgggtgtt tcatcttatc tgcttatcgg
    20281 attttactac acaaaaccat cagcggttgc tgcatcgaaa aaagcattta tcgttacgag
    20341 gtttgccgat tttttcttcc ttatcggaat acttgtacta tcctacctca cgcagacttt
    20401 tgatttcggt acgctcctgt cgggcgacgg cagtatattc aacaatgcac ttggttctac
    20461 tttcatgggt gcttcggctc taacctgggc catggcactc atattcatcg gaggtgccgg
    20521 aaaatcggcc atgttcccgc tgcatatctg gcttccggat gccatggaag gtcctacgcc
    20581 agtttctgcg cttatacatg cagccacgat ggtggtagcc ggtgtatatc tggtagcccg
    20641 tcttttcccc atttatattg gttttacccc cgaaatactt catggaattg cctatatcgg
    20701 agcatttaca gctatgttct cggccgtaat cgctattgta cagacagata ttaagcgggt
    20761 actcgctttt tccaccatat ctcaaatcgg atttatgatg gtggcgctcg gtgtgagtac
    20821 aagtatggag ctcaatcacc acgaacacgg cggactgggt tatatggctt ccatgtttca
    20881 tttgtttact catgccatgt tcaaggctct gttattcctc ggagccggtt caatcattca
    20941 tgccgtacac agcaacgata tgaaggatat gggaggttta cgaaaattca tgcctgttac
    21001 tcatattaca ttcctcatag cttgtcttgc tattgccggg attccaccat tttccggatt
    21061 tgtaagcaaa gacgagataa tgacagctgc atatcatttc agcaccggaa tgggtgtttg
    21121 gatgactata acagccggta ttacagcatt ttacatgttc cgtctttatt ataatatttt
    21181 ctggggcaag gaatataaag ttcatggaaa tcataaacct cacgaaagcc cgctttcgat
    21241 gacttttccg ctttcatttc tggcattgct taccgtggtg ggtggtttta ttccgttcgg
    21301 aaaattcgtt actcccgacc gcagcgaata cctgattcat ttggatataa aaatcgccgt
    21361 tatcagtgtg gctgttgcgg taacggcaat tgcactggct actttactat atcgcagaga
    21421 cagcaaacgt ccggcagaac tggcgggaaa aatgagcggg ctgtacactg ttgcttacaa
    21481 taaattttat atcgacgagg tgtatcagtt tattaccaaa aaaattatct tcaacagtat
    21541 atcacgtccg tttgcctggt ttgatcgcca tgttgtagat ggtgccatga acggaatggc
    21601 acgacttacc ggatggactt cggtgaaaac caaaggtttc cagtccggca gtattcagtg
    21661 gtatgcatgg gttttccttt ttggaacatt attaattact gcattagcag cgtttttata
    21721 gaaaactgat tttctgtttt taatttgtaa tttataaagt taggatatga actttcttct
    21781 tattttcgta ctcataccgg tattaatgac actctcgctg tttctgacaa ggaaccgaaa
    21841 acaggtactg ggtattatgg tgaccggcag cattgcactg ctggctgctt cggcgtatct
    21901 tgtggttgct tatgtttctg cccgtaatgc cggtgcatcc gacccgatgc ttttcacttc
    21961 cagtatgatg tggtacaaac ctctcaatat cggattttcg cttggagttg acggggtatc
    22021 tgttgctatg ttgtgtcttt catctgtcat tgtactgaca ggtacattta cttcctggca
    22081 aatagccccg ttgaccaaag aatttttcct gtggttttgt ctgctttcaa ccggcgtttt
    22141 cggatttttc atcactaccg atttattcac catgttcatg ttttatgagg tggctctcat
    22201 tccgatgtac ctgctgatag gtatctgggg tagt
//
LOCUS       DAORKP010000015        21799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_98_length_21799_cov_7.330114, whole genome shotgun sequence.
ACCESSION   DAORKP010000015 DAORKP010000000
VERSION     DAORKP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21799
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_21799_cov_7.330114"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLN75_01805"
     CDS             complement(<1..586)
                     /locus_tag="PLN75_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRG02881.1"
                     /translation="MKTKFVYFISVLSVFSLLYACKEPPVTLSNAYIESLSVNGKNLA
                     SDGTAMNVPVDSVIIRAVFSTEIDNSKINPDKIYVSNDVGIEIEILTSENPKELKLKI
                     KNKLNYYTKYTVFINSGEHLGVNVIDNSKVHFITQLDITPKFPAISDDNLLTLVQQKT
                     FEYFREYAHPVSGLARERTGSGETVTTGGSGFGLM"
     gene            complement(663..1544)
                     /locus_tag="PLN75_01810"
     CDS             complement(663..1544)
                     /locus_tag="PLN75_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRG02882.1"
                     /translation="MKTLKIFGALAILTFAIVFSGCKKEEQPLPPIGGYNNADEVGKA
                     DLVAYWPLNGDGKESLSSSAPAKSSNVTWVDGIKGKAASFNVGYLAYNTIANLGTNLN
                     SAFTVSSWVKLTNNGANSSVIFSLSRPNEWAGSINFMSETGWMPATSDSLTVKGLIVS
                     STDLGWQDSRNTVKANAADITAGHVPYPNKIGGKWAHVVLTWNNTTKLLKVYVNGTKI
                     SNPVWENRDATGTKNFVVTTPCHPILGAFETFANGTTTDAWNTGLKGDMDEVRVWKRE
                     LILADIGALYELEKAGR"
     gene            complement(1614..3068)
                     /locus_tag="PLN75_01815"
     CDS             complement(1614..3068)
                     /locus_tag="PLN75_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG02883.1"
                     /translation="MKHFKIFSIITFCLTLLLVGCEKDLNVLPQDKMSKDDFLAEPLY
                     AVEVVNSAYNQMLSFEMYGFPWIGVTSITSDDADKGSSDGDAGTDKDKLDNLAFDKSL
                     RSFNDVWKRRYEGIYRCNEGIFYLGQVSLEESIKNRYIGELKFLRAMFYFDLVRCFGG
                     VPVVVEKLDFKQEATVNQVVYSRKTKAETYAQIEKDLIDAAASLPLKYNGENIGRATK
                     GAAQGLLAKVYLYQEKWSDAYAMAGNVVTSKMYDLLPDYASVWREVGENGKESLFEIQ
                     ATLGKGVNGYTDVQSGRGTPDHGWGFNTPTANLANAYEPGDKRKDGTIMFTGQVLWDG
                     FQTPTTGWANPRYNYKAYQSRSQESWDDNKGETAKNVRLLKYSDILLVRAEAAHHINQ
                     LDTALSIVNKIRNRAGLIPLTSVTLDQIYKERRLEMAMEHDRWFDIVRTGKASASMSA
                     VGKVFIQGKHELFPIPEEQITLSKGILTQNPNYN"
     gene            complement(3114..6098)
                     /locus_tag="PLN75_01820"
     CDS             complement(3114..6098)
                     /locus_tag="PLN75_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG02884.1"
                     /translation="MNSKFSTFFLLMLLSVFPLIAQNVTITGVISDKVTGETLIGAKV
                     LQKGTNNGTITNFDGNFTISVPLNSILEISYVGFQSKEVTVSSNAPLVIVLETNDKIL
                     DEVVVVGYGTKKAGAITGSVVQIKSDDITKTPAQSAIQSIQGKAAGVNIIATDEPGRA
                     PTVMIRGINTVLGGGTPLYVIDGTEASSLNGLSANDIETIDVLKDASSLAIYGNKAAS
                     GVILVTTKKGKKGSIKVNYDGYVGFKTMMKTVDMADAYHFAYYQNFAAGRSRFATTQP
                     YETNWLKEITQIGTTNNHSVSLSGGEENVNYYFGISRYDEKGILKGTDYDRTNILSKN
                     EYKLFNRLKLSQSINISVVNNVPQPSSAFTAAYKQSPIMPVKYDNGKWGMPFLDTSTG
                     EIGMIGDKFNNVPNPVAMLGYNEDKNRNVTLFGTLGAEIEIYKDLKFNSNFGATFDYG
                     MGYSYTPNKENWFSQNPTATEDDFYKNNKFYSTLSQSRSHFFVWNWDNFLTYSKDFDK
                     HGFTGVLGITRSTTRTGQYLSGRRNNVPYQPNYWSLDLAKSNITTSPDNVVSNRSQTP
                     LVNLGYFARVDYDYDRKYLLTASLRRDGISDFQSSKKWGFFPAVSAGWVVSSEDFFEK
                     YASVVDYLKIKAGYGEIGNGNAAPSVNQILFQNGATYPFGSPEIIYPGSFVPYAVDPD
                     LTWETTKEFGGGIEFRMLKQRLFGTLEFYRKLTDNVILPVKLPTVLSPGDVYLNAGTV
                     ENKGIEATFSWNDKLGDEFNYRAGVNFAVNSNAIKEIYSSYFDKLTGGDLGNGHYTKE
                     IHIGDPIGSFYVYEHTGYTEDGVFAYSSERINAGSYIPKLTYGFNLGFDYKNFDFSTD
                     WYGVMGNKVYNGKKAQRWGGENVEMNEFNDFWIPSNPKQDAANPRPYSDTPMASTYFI
                     EKGDYLRINNVTIGYTLPKIVKELQSLRIYLTAVNPFIFTKYTGFTPELIGSGTPLGS
                     AGIELDAYPTNRSLTVGLNLSL"
     gene            complement(6104..7693)
                     /locus_tag="PLN75_01825"
     CDS             complement(6104..7693)
                     /locus_tag="PLN75_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRG02885.1"
                     /translation="MKKITNIGIFVLLSLTLQAQGKFIVPNDLKAQNFPGHTEITWKN
                     HQGFTYEVYRMTGNSKAFVKQGETTKGYYLDFYGKNIPQNARIRYRIVPKGMNIASND
                     AAKFETTITRKAYTDDDLLDMTQRYTTRYFYDFAEPYTGMARERSNDINGDIVTTGGT
                     GFGLMALVAGIERNYFPKEKGLDIIGKIVNFLGSVERFHGAWAHWYDADTRKAFSFSK
                     YDDGGDLVETALLMQGLLTAREYLKKGNEAERDLSGKITHLWETVEWNWYTQGTDSLY
                     WHWSKNYGWKMNHRIKGFDETLITYVLAASSPTFPIGRNVYEACFKNSPYYYNGKSYF
                     GIKLDLGMDYGGPLFFTHYSFLGLNPNGLTDSHTNYFERNKAHALIHRAYAISNPKKH
                     PGYGAKLWGFTSSDDPLVGYSSHHPGTNDDNGTVPPTAAISSIVYTPKESMEVLKYLY
                     FEKGKQVFGKYGFYDAYNPGMTEGQQVVKSYLAIDQGPIAVMIENHRSSLIWDLFMGN
                     PEIQNGLTKLGFDFQLTSHKK"
     gene            complement(7697..10078)
                     /gene="bglX"
                     /locus_tag="PLN75_01830"
     CDS             complement(7697..10078)
                     /gene="bglX"
                     /locus_tag="PLN75_01830"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRG02886.1"
                     /translation="MKNPSKFLLLIIIFTFCFIPYQEVSAQKKSKKKQTIAAIPLVPA
                     KPISTKSIHFKSDPVMDQFIDDLMSKMTLDEKIGQLNLPSAGDFTTGQAKSSNIGQSI
                     KEGKVGGLFNIKGVEKIREVQRVAIEESRMKIPMLFGMDVIHGYESTFPIPLGLASSW
                     DMDLIEQSARVAAQEATADGINWTFSPMVDISRDPRWGRISEGSGEDPYLGSRIARAM
                     VTGYQGNDLSATNTMLACVKHFALYGAPEAGRDYNTVDMSRVTMYNVYFPPYKAAVDA
                     GVGSIMASFNEVEGIPATGNYWLQNQVLRKEWGFKGLNVTDYTGIKEMIEHGVGDMQA
                     SSALALRAGVDMDMVSEGFLSTLKKSLQEGKVTYSEIDQAARRILEAKYRLGLFADPY
                     KYCDSERAKKEVYSTDNRAIARSTAAQSMVLLKNNNQLLPLQAKGKIAVIGPLADNPE
                     NMPGTWSVASKHSNAVSLLKGLKETYGKQVEFLYAKGANFDYDPEFEGKITMFGKKAY
                     RDERPKEEILKEAVEAANRADVIIAALGEASESSGEAASMVNLELRQSQKDLLAALKK
                     TGKPIVLVLFNGRPMVINDEIEKADALLNVWFPGSEAGYAISDVLFGKVNPSAKLPVT
                     FPRNVGQIPLYYNHKNTGRPLTDENVWEKFRTAYLDSPNLPLFPFGFGLSYTTFTYSD
                     INVSAKNLQGNGKLTASVTLTNTGKYDGAEVVQLYIRDVVGTNTRPLKELKGFQKVML
                     KAGESKTVSFEITPEDLKYYNNELKFDWESGDFQIMIGGNSQDVKSITINWEK"
     gene            complement(10489..13389)
                     /locus_tag="PLN75_01835"
     CDS             complement(10489..13389)
                     /locus_tag="PLN75_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRG02887.1"
                     /translation="MINSYFKIGFMKLLKVRIIFFFLYSSFLLAANDFTPVVTQFNKK
                     DYNAGNQNWSVAQDSQGLIYFGNNVGLLRFDGSGFTLFKLPNNQVVRSVYIDKSDRIY
                     VGSFREFGYFEKDKFGELVYSSLSNDLKNYKLENDEIWKIIEFDGKILFQSFKSIFIY
                     DGKNVEGKSYDETFLYLQRFRNKLYVHTLQNGLSSFDPKNKKFTKIENTPFSSAVISI
                     IQFNKNEAYVITLSDGIYIFDGQDFRKFSDGSLDYLRNAEINKATLTRDSLLVIGTIL
                     DGVSAIDKNGKKVWKLNTSNNLQNNTVLGMSTDSENNLWLALDKGIAMVRLNSPVQFV
                     QSFTPSIGSIYSLSYLPPDKLYLATNQGLYSGRFSPDSKSLTNIQLDSQIKGQVWSLT
                     NVDNQLFCGNNEETYRIYPDRKMLSTSKGGMCIGRGIIHGREVLVEGTYSDLCVYLKE
                     NNEWKFHSVIQGFLNPVKSITIDYQGRIWASHMHEGLYMIMLKSDLKTIDKIQKFTSL
                     DNKKSSLIYTYMFQNRVVFTDNTRFYVFDDIKNKILPFDELNTTLGKYARAYKICIQK
                     PSTYWFILEKEAALIQFIDNRAQILDIVQYSLFMNQTVDNFQNIIPLTEDIAMFTLEN
                     GLAMYNLKLRKQSLYHSKKEILLMQVQVVDKNSEEMMLLPVSSANEKISFPYTKNRIR
                     FTIGYPQYSNLNDLLFSYKLTGLNNNWSESTTSNMKEYSYLRPGKYTFSVRVVSNNGD
                     VFATRDYLFRVRSPYYWNTFSKIIYVLLLILTGFIIYNLLKRNFEQKKQRIQQEQENI
                     RKKEIEKREMQIVALKAEKLESDLTLKSKELAMSTITVIRKNEVLINIKDELIELKKH
                     LGTQFPNKYYERIIKLMDQSISSDDDWKIFQTNFDRIHENFFRNLRTKYPELTPNDLR
                     FCAYFRLNLSTKDVAHLMNISAKGVEVARYRIRKKINIPSEKNISEFLIEFR"
     gene            complement(13635..15203)
                     /gene="xylE"
                     /locus_tag="PLN75_01840"
     CDS             complement(13635..15203)
                     /gene="xylE"
                     /locus_tag="PLN75_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HRG02888.1"
                     /translation="MSGNKKNPFYLAGITLVATLGGLLFGYDTAVISGTVSSLRIFFV
                     DPLGLSPDLANAMEGFIVSSALIGCVIGASIAGYVSQKFGRKPSLQIAAVLFLLSAVG
                     SAWPEMFFGMPGTGDHTFANLFIFYRIIGGVGVGIASMVSPMYIAEVAPHNKRGALVS
                     WNQFAIIFGMLVVYFVNYAIALQGDSSWLNTIGWRWMFASEIIPASLFLIFLFSVPET
                     PRWLVMNSKKDKARSVLVKLVGSQEADVEIDDIQLSLDQDAAQSLKPYYRFLVTWILL
                     FTIAFIGLKFAGNASAFEISLIGSFVISLIAPIRSFGFKIIIVGVLLSAFQQFVGINV
                     VLYYAPEIFKSMGSGTDTALLQTIIVGIINLSFTVLAILTVDKFGRRPLLIIGALVMA
                     LAMTGLGTGFAGSWSGGLKLFFMLLYTAGFAMSWGPVCWVMLAEIFPNSVRSSVMSIA
                     VAAQWVSNFLVSWTFPMLNKNEALVSQFNHGFSYWVYGLMGILAAIFIWKMVPETKGK
                     TLEEMNKLWKKKES"
     gene            complement(15214..16533)
                     /gene="xylA"
                     /locus_tag="PLN75_01845"
     CDS             complement(15214..16533)
                     /gene="xylA"
                     /locus_tag="PLN75_01845"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRG02889.1"
                     /translation="MTTKTYFPTVEKIRFEGKESKNPLAFRYYDAEKVVYGRKMKDWF
                     KFSMAWWHTMCSESSDPFGGETKSFEWNKADDALQAAREKMDAGFEFMQKIGIEYFCF
                     HDIDLVDEGNSIEEYEANLKAIVAYAKEKMAQTGIKLLWGTANVFSHKRYMNGAATNP
                     NFDSVSRAAVQIKNALDATIELGGENYVFWGGREGYMSLLNTDMKREKEHLAQMLKMA
                     RDYARGKGFKGTFFIEPKPMEPTKHQYDVDAETVIGFLRAHGLDKDFKLNVEVNHATL
                     AGHTFEHDLQCAADAGMLGSMDANRGDAQNGWDTDQFPIDVYELTQAMLVVLKAGGLQ
                     GGGTNFDAKTRRNSTDLEDIFIAHIAGMDAFARALEAAAGILEESPMPGMLKERYASF
                     DGGIGKDFEDGKLTLEDVYAYAKAKNEEPAQISGKQELYESIINMYI"
     gene            complement(16581..18056)
                     /locus_tag="PLN75_01850"
     CDS             complement(16581..18056)
                     /locus_tag="PLN75_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRG02890.1"
                     /translation="MYLLGYDLGSSSVKASLVNAKSGEVMASDFFPKKEMEIKAVKAG
                     WAEQDVEVWWSNLKEATRSVLATAGIDPKEIDAIGISYQMHGLVLVDENLQAIRPSII
                     WCDSRSIAYGEKAFNVIGSEKCLSHLLNSPGNFTLSKLAWVKENEPENYSRIKYYMLP
                     GDYIALRMSGHPATTASGLSEGISWDFLNNRPAGFLYEHYGFSEEFIPEIVPTFGHQG
                     RVSAEIAAELGLSEGIPITYRAGDQPNNALSLNVLNPGEVAATAGTSGVVYGVNDDAK
                     YDPQSRVNSFAHVNHCSEATRIGVLLCINGTAILNTWIKNNVAPEGISYAEMNDLAAK
                     VPVGSEGISIIPFGNGAERVLNNEEPNCSIHGINFLVHGKSHLIRAAHEGIAFSFKYG
                     MDIMNKMGIETKTIRAGHANLFLSPIFRQTLSNITGATIELYNTDGSIGAARGAGIGA
                     GIYQSAEEAFSTLKKVLVVTPEEKDVEAAQDAYSLWISRLK"
     gene            complement(18378..19082)
                     /locus_tag="PLN75_01855"
     CDS             complement(18378..19082)
                     /locus_tag="PLN75_01855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRG02891.1"
                     /translation="MSETRPYNPYVSIDCVVFGFDGKNLKVLLIKRHIDENTGEYNDV
                     KLPGSIIYMDEDLDEAATRVLAELTGLKNIPLSQFYSFGNPLRTSNPRDILWLERTMK
                     TKIERIVTIGYVALIKINRKITLNSKNTSAEWYDVDEVQNMNLAMDHNLIVQKGLDHI
                     KHWLTVEPHLMFKLLPHKFTLSQLRLLYDEINRTKSDVRNFQKKINQWHNLVVLDEFE
                     KEVPHRAGRLYKYQKK"
     gene            19499..20959
                     /locus_tag="PLN75_01860"
     CDS             19499..20959
                     /locus_tag="PLN75_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRG02892.1"
                     /translation="MKTNNENRRGSYGSDASRDGKSFDRKNAKPLLKGIRTSSPSNFG
                     SRNQDNRYDRTANNDRNRFSDRSENRYERKDDRPNSYSERKPYINRNNDDRRSEPGSE
                     RKFFVNRNTDNRRTDSDSDRRHYSSRNNDDRRNDSGQERKPWLNKDTNRQSSGRPRFI
                     RKSEVGDKPAEYSTPRKRFDSDERPRDGKLTFRKAGEVKDSNYVETRFDNKKNGITFR
                     KRHNSADYDPNAKYSKKKQFEYRQSYIDPTKPIRLNKYLSNAGICSRREADEYIQAGV
                     ISVNGNIVTELGTKVLPTDNVMFHNQPVRIEKKVYILLNKPKDCVTTSEDTQARTTVI
                     DIVKNACSERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPKYDKKKIYHVGLDRDLER
                     EDFEAILDGIDLGEFTIKADALEFVKDGSLREIGIEIHSGQNRVVRRIFDKLGYKITK
                     LDRVFFAGLTKKNVPRGKYRFLTEKEVNILMMGAHE"
     gene            21047..21607
                     /locus_tag="PLN75_01865"
     CDS             21047..21607
                     /locus_tag="PLN75_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02893.1"
                     /translation="MATQQTSDEPVNQVSTTTTTKRKPGKVVKYILNHKLAFSLLFAL
                     IVVFIWAQWQIHKLEKEKQALTETYKMQIDSLRQADYMLVSKVFSWAVRSDMMRNNYD
                     QANQYIDFIIREPHILKAFAIDAATNTIVLSSDKNEVGLPVADITLLRPVENAMVRSD
                     STVRFVTPITALNQKAGVSVLVTNLK"
BASE COUNT         6042 a         4728 c         4362 g         6667 t
ORIGIN      
        1 ccataagacc aaaaccggaa ccgcccgtag tgactgtttc gccggaacct gtacgttcac
       61 gtgccagccc tgaaaccgga tgagcatatt cccggaagta ttcaaaagtt ttctgctgaa
      121 caagtgtaag taaattatcg tcactgatag ccggaaattt tggggtgata tccagttgcg
      181 tgatgaaatg aaccttactg ttgtctatta cattgacgcc taaatgttcg ccagaattta
      241 taaaaacggt atatttagtg taataattaa gcttattttt aattttaagt tttaattctt
      301 ttggattttc cgaagtcaaa atttctattt caattcccac gtcgttgctg acatagattt
      361 tatccgggtt gattttcgaa ttgtcaattt ctgtagaaaa aacagcacgg attataaccg
      421 aatcaaccgg cacattcatg gcagtaccat ccgacgccag gttttttcca ttcaccgata
      481 atgattcgat ataggcattg gatagcgtta ccggtggttc tttacacgca tagagcaaag
      541 aaaaaaccga tagtacggag ataaaataaa cgaattttgt tttcattgga taataaatga
      601 taaactgtgg gaaaaatccc acagtttatt ttacagactg ataatttact tactcaaatg
      661 atttatctgc ctgctttttc gagttcataa agagcgccga tatcagctaa aatcagttca
      721 cgtttccata cacgtacttc gtccatgtcg cctttcagtc cggtattcca tgcatcggta
      781 gtagtaccgt tggcaaatgt ttcaaacgct ccgagaatag gatgacaagg agtagtaaca
      841 acaaagtttt tagtacctgt tgcgtctctg ttttcccaaa ccggatttga aatttttgta
      901 ccgtttacat acactttcag aagttttgtt gtgttattcc aggtaagaac cacgtgtgcc
      961 cattttcctc cgattttgtt gggataaggt acatgaccgg ctgtaatatc agctgcgttt
     1021 gctttaaccg tgtttcttga atcctgccat cccaaatcgg tacttgaaac gataagacct
     1081 ttcacggtta agctgtcgct ggttgcaggc atccagcctg tttctgacat gaagttgata
     1141 cttcctgccc actcgttagg acgtgacaag gagaaaatca cagaagaatt tgctccgtta
     1201 ttcgtgagtt tcacccatga acttacagta aaagcactgt taaggtttgt acctaagttg
     1261 gctatggtat tatatgccag gtatcccaca ttgaaacttg cagctttacc tttaatacca
     1321 tctacccagg ttacattgct tgatttggca ggtgccgaac ttgataatga ttctttacca
     1381 tcaccattta acggccagta ggctaccaaa tctgctttac ctacttcgtc ggcattgttg
     1441 taaccaccaa tgggtggtag tggctgttct tcttttttac aacctgaaaa aacgatagca
     1501 aaagttaaaa ttgccagtgc accaaaaatt tttaatgttt tcatttttct ttgttttttt
     1561 taaatcgggt taattaattt ttttgttaaa tcattgattt ataaataatt ttcctaatta
     1621 taattaggat tctgagtaag tattcctttg cttaatgtga tttgttcttc agggattgga
     1681 aacagttcat gttttccctg aataaatact ttgcctactg ccgacatgga agcagaggct
     1741 ttaccggttc tgactatatc gaaccatcga tcgtgttcca tggccatttc caaccggcgt
     1801 tctttgtaaa tctggtctaa ggtcacggag gttagtggaa tcaatccggc acgatttctg
     1861 attttattaa cgatagataa ggctgtatcc aactgattaa tatggtgtgc ggcttcggct
     1921 cttaccaaca atatatcgct gtatttcaat aatcgaacat ttttggctgt ttcacctttg
     1981 ttatcgtccc acgattcctg tgagcggctt tgatatgctt tgtaattata ccttggattt
     2041 gcccagcctg tagtcggagt ctgaaagcca tcccaaagta cctgtccggt aaacataatg
     2101 gtaccgtctt tccgtttatc tccgggttca taggcatttg ccagatttgc agttggtgta
     2161 ttgaatcccc agccatggtc aggagttccg cgtccgctct gtacgtcggt atatccgttt
     2221 actcctttgc ccaatgtagc ctgtatctca aaaagtgatt ctttgccatt ctcgcctact
     2281 tcacgccaca cactggcgta atcgggcaat aaatcataca tcttgcttgt tactacattt
     2341 cctgccattg cataagcatc gctccatttt tcctgataca ggtatacttt agccaaaagt
     2401 ccctgagcag cacctttggt agctctacct atattttctc cgttatattt caatggcaac
     2461 gatgcggcag catctatcag gtctttttca atttgagcat aggtttcggc ttttgtttta
     2521 cgtgaataaa ctacctgatt gacagtggct tcctgtttga aatccagttt ttcgaccacc
     2581 accggtacac cgccaaaaca tctgaccaaa tcaaagtaga acatcgcccg aagaaatttt
     2641 aattcgccaa tatagcgatt tttaatactt tcttccagac ttacctgtcc caagtaaaaa
     2701 ataccttcgt tacagcggta aattccttcg tatcgccttt tccaaacatc gttgaatgaa
     2761 cgcagtgatt tatcaaaagc cagattatca agtttgtctt tatctgttcc ggcatcacca
     2821 tccgacgaac ctttatcggc atcatccgaa gtgatactcg ttactccaat ccaggggaag
     2881 ccatacattt cgaaactcaa catctgatta taagcagaat tgacaacttc tacagcatac
     2941 aatggttctg ccagaaaatc gtctttcgac attttatcct gaggaagcac atttaaatcc
     3001 ttttcgcagc cgactaataa cagggtcagg caaaaagtaa taatggaaaa tatcttaaag
     3061 tgtttcataa tttcttgtgt taattacttg tttttaatgt tcaatccact tttttacaat
     3121 gataaattca gtccaacggt gagtgatctg tttgtgggat aagcatcgag ctcaatacca
     3181 gccgaaccga gcggagtacc tgaaccaatc aactctggtg taaatccggt atatttggtg
     3241 aaaataaacg gattaacagc cgtaagatat attctcaggc tttgtaattc ttttactatt
     3301 ttcgggagtg tgtatcctat cgttacgttg tttattctca gataatctcc tttttcaata
     3361 aaataggtag atgccattgg agtatcggaa tatggtcttg gattggcagc gtcctgtttc
     3421 ggattggatg gaatccagaa atcgttgaac tcattcattt ccacattttc tccaccccat
     3481 cgttgtgctt ttttaccatt gtagacttta tttcccatta caccgtacca atcggttgag
     3541 aaatcaaaat tcttgtaatc aaagcccaga ttaaacccgt aagtcagttt cggaatatac
     3601 gagcctgcat ttattctttc ggaagagtaa gcaaatacac catcttcggt atagcctgta
     3661 tgttcgtata catagaatga gccaatcggg tcgccaatat gtatttcttt tgtgtaatgc
     3721 ccgttgccta aatctccacc ggttagtttg tcaaaatatg aactgtatat ttcttttatt
     3781 gcattactat ttacagcaaa attcacacca gctctgtaat taaattcatc acccagtttg
     3841 tcattccagg agaatgtggc ctcaatccct ttgttctcaa ccgttccggc attcagatac
     3901 acatcaccgg gcgaaagtac ggtgggaagt tttacaggta aaatcacatt atcggtcaat
     3961 ttacgataaa attccagtgt accgaataat cgttgtttca gcattctgaa ttcaataccg
     4021 cctccgaatt ccttcgttgt ttcccaggtt aaatcgggat cgactgcata cggtacaaac
     4081 gaacccggat aaataatttc gggcgagccg aaaggataag tggctccgtt ttggaaaagt
     4141 atctgattga ccgatggagc tgcgtttccg ttacctattt caccatatcc ggctttaatt
     4201 ttcaaataat caaccactga tgcatacttt tcaaagaaat cttcgctgct cactacccat
     4261 cccgccgaaa ctgccgggaa aaatccccat ttttttgacg attgaaaatc actgatacca
     4321 tcccggcgaa gcgaggcggt caataaatat tttcggtcat aatcatagtc aacacgtgca
     4381 aaataaccca gattgacaag cggagtctgg cttctgtttg aaactacgtt atcgggtgaa
     4441 gttgtgatgt tacttttggc aagatccaac gaccagtaat tgggctgata aggtacgtta
     4501 ttacgccttc ctgacagata ttgtccggtt ctggtagtag aacgtgtgat acctaaaacg
     4561 ccggtaaagc cgtgtttatc aaagtctttg gaataggtaa gaaaattatc ccagttccac
     4621 acgaaaaaat gacttctgct ttgacttaac gtactgtaga atttattgtt tttgtaaaaa
     4681 tcatcttcag ttgcggtggg attctggctg aaccaatttt ctttgttcgg cgtataagaa
     4741 tatcccattc cataatcaaa ggtagccccg aaattagaat taaatttcag gtctttgtaa
     4801 atttcaattt cggctcccaa agtaccaaat aaagtgacat ttctgttttt gtcttcgttg
     4861 tatccaagca tggcaacggg gtttggtacg ttgttaaatt tatctccaat catgcctatt
     4921 tcgccggtgg aagtgtccag aaatggcatt ccccattttc cattgtcgta ttttacgggc
     4981 ataatcggcg attgcttgta agctgccgta aatgcacttg atggttgcgg aacgttattt
     5041 actaccgaaa tattgatgga ttgcgatagt ttcagccgat tgaaaagttt gtattcattt
     5101 ttgctcagga tgttcgttct gtcataatca gtacctttca gaatcccttt ttcgtcgtag
     5161 cgcgaaatgc cgaaatagta attcacgttt tcttcgcccc ctgaaagtga gacagaatga
     5221 ttatttgtcg ttccgatttg agttatttcc ttgagccaat tcgtttcgta aggttgagta
     5281 gttgcaaaac gggatctccc ggcagcaaaa ttctgataat aggcaaaatg atatgcatcg
     5341 gccatatcta ccgttttcat cattgttttg aagccaacgt acccgtcata attcactttg
     5401 atacttcctt ttttcccttt tttagttgtc accaaaatta caccgcttgc agctttattg
     5461 ccataaattg ccagcgaaga agcatccttg agtacatcaa tagtttcgat atcattagca
     5521 ctcagtccgt taagactgct tgcttcagtt ccgtcaatta catacagggg agttccaccg
     5581 cccagcacag tattaatacc acgtatcatt acagttggtg cccgtccggg ttcgtcagtg
     5641 gcaataatgt ttacacctgc tgctttcccc tgaatggatt gaatggctga ttgggccgga
     5701 gttttcgtga tatcatccga tttaatctga acaacagacc cggtaatggc tccggctttt
     5761 ttagtaccgt aacctactac cactacttca tccagtatct tgtcgttggt ttccagcaca
     5821 atgacaagcg gtgcgttgga gctaaccgtt acttccttac tttgaaaacc aacatagctg
     5881 atttcaagta tggagttgag cggtacggaa atggtgaaat ttccgtcaaa attagtaata
     5941 gtaccattat ttgttccctt ttggagcact ttggctccaa taagtgtttc accggtaact
     6001 ttatccgaaa ttactcctgt aatggtaaca ttttgagcta ttaacggaaa aaccgataaa
     6061 agcatcaata aaaagaatgt tgaaaatttg ctattcatct gtattacttt ttatgtgaag
     6121 ttaattgaaa atcaaatcct aatttcgtta gtccgttttg aatttccgga tttcccataa
     6181 ataaatccca aatcagtgag ctccgatggt tttcaatcat cacggctatg ggcccctggt
     6241 caatggcaag gtagcttttc accacctgct ggccttcggt catacccgga ttgtatgcat
     6301 cgtagaatcc gtattttcca aaaacctgtt ttcctttttc aaagtaaagg tatttaagta
     6361 cctccatgct ttctttcggt gtgtaaacta tggatgaaat ggctgcagta ggcggcactg
     6421 tgccgttgtc gtcgttcgta cccggatgat gcgacgaata tcccaccagg ggatcgtctg
     6481 aggatgtaaa tccccacagt ttggcaccgt aaccgggatg ctttttcgga ttactgatgg
     6541 cataagcccg gtgaatgagc gcatgagctt tgtttctttc gaaatagttc gtgtggctgt
     6601 ccgtcaggcc atttggatta agtcccagaa agctgtaatg ggtgaaaaac agcggcccgc
     6661 cataatccat tcccagatcg agttttattc cgaaatagga cttgccgttg taataatagg
     6721 gcgaattctt gaaacaggct tcgtacacat ttctcccgat ggggaatgta ggtgatgaag
     6781 ctgccagcac gtaagttatc agtgtttcgt cgaaaccctt gatacggtgg ttcattttcc
     6841 agccgtaatt tttggaccag tgccagtaaa gcgagtcagt gccctgtgtg taccagttcc
     6901 attccacagt ttcccagaga tgagttattt ttccggacaa atcccgttcc gcctcattac
     6961 ctttttttag gtattcgcgg gcagtgagca gtccctgcat aagcagtgcc gtttccacca
     7021 gatcgcctcc gtcgtcgtac ttactgaagc tgaacgcttt acgtgtatcg gcatcgtacc
     7081 agtgtgccca ggcgccgtga aaccgctcca cgctgcccag aaagtttaca atttttccga
     7141 tgatgtccag tcctttttct ttgggaaaat aattgcgttc aattcctgct accagcgcca
     7201 tcagcccgaa accggttcca ccggtcgtta cgatatcgcc atttatatca ttactccttt
     7261 cgcgtgccat tccggtgtaa ggttcggcaa aatcatagaa ataccgggtc gtatagcgtt
     7321 gcgtcatatc aagcagatcg tcgtcagtgt atgcctttcg ggttatggtt gtttcaaatt
     7381 tcgcagcatc atttgaagct atattcatcc ctttcggaac aatccgatat cgaatccggg
     7441 cattctgtgg aatgtttttt ccgtagaaat caaggtaata tccttttgtg gtttctccct
     7501 gttttacaaa tgctttcgaa tttcctgtca tccgatacac ctcataggta aatccctgat
     7561 gatttttcca ggtaatttcg gtatgtcccg gaaagttttg tgctttaaga tcattaggaa
     7621 caatgaattt tccctgtgcc tgcagggtca gactcagcaa tacaaaaata ccaatattag
     7681 tgattttttt cattgtttat ttttcccagt ttatggttat cgatttcaca tcctgcgagt
     7741 tacctccaat catgatttga aaatcgccac tttcccagtc gaatttcaat tcattgttgt
     7801 agtatttcag gtcttcggga gttatctcaa aactgactgt tttgctttcg ccggctttga
     7861 gcatcacttt ttgaaaacct ttcagttctt tgagcggacg ggtgtttgtt cccactacgt
     7921 cacgtatata taattgtact acttctgctc catcgtattt tcctgtgttg gtgagagtta
     7981 cgctggctgt cagttttccg tttccctgca gatttttagc tgacacgtta atatcgctat
     8041 aagtaaatgt agtgtagctc agtccgaatc caaaggggaa taatggtaaa ttgggtgaat
     8101 cgaggtatgc tgtgcggaat ttttcccaca cattttcatc ggtaagcggt ctgccggtat
     8161 ttttatgatt gtagtaaagg ggtatctgtc ctacatttcg ggggaatgta accggaagtt
     8221 tagccgaagg attcactttt ccgaacagca catcggaaat ggcataaccg gcttcacttc
     8281 cgggaaacca tacatttagt aatgcatcag ctttttctat ttcatcattt attaccatgg
     8341 gccggccatt aaagagcacc agcacaatcg gttttcccgt ttttttcaat gctgcgagca
     8401 ggtctttttg cgactgccgg agttccagat ttaccatcga tgcggcttcg cccgacgatt
     8461 cggaagcttc gcccaatgca gctataataa catctgcacg gtttgctgct tcaacagctt
     8521 ctttgagaat ttcttctttc ggacgctcgt cgcggtaggc ttttttgcca aacatggtta
     8581 ttttgccttc aaattcggga tcgtaatcaa aattagcccc tttggcatat aaaaactcaa
     8641 cctgcttgcc gtaggtttct tttaaacctt taagaagaga aacagcattg ctgtgtttgg
     8701 aagccacgct ccacgttccg ggcatatttt ccggattatc ggcaagcgga ccgataacgg
     8761 ctatttttcc ttttgcctga agtggcagaa gttgattgtt gtttttaagc agtaccatcg
     8821 actgagcagc tgtgctacgg gcaatggctc tgttatcagt tgaatatact tcctttttag
     8881 ccctttccga atcgcaatat ttatatggat cggcgaaaag tccaagtctg tatttggctt
     8941 ccagaattct tctggcagcc tggtcaattt ctgaataagt gactttacct tcttgcagtg
     9001 attttttaag tgtcgacaga aatccttcgc tcaccatatc catatccact cccgcacgca
     9061 aagccagtgc agatgaggcc tgcatatcac ccacgccgtg ttctatcatt tctttgatgc
     9121 ctgtatagtc ggtcacattc agtcctttga atccccattc tttacgcaga acctggtttt
     9181 gaagccagta gtttccggtt gcaggtattc cttccacttc attgaaagag gccataatgc
     9241 ttccaacacc tgcatctaca gcggctttat aaggtggaaa atacacatta tacatcgtta
     9301 cccggctcat atccaccgtg ttgtagtcgc gaccggcctc cggagctccg tagagtgcaa
     9361 agtgttttac acatgcgagc atcgtattag tggccgacaa atcattaccc tgataacctg
     9421 taaccatagc gcgggcaatc ctgctgccca gatacggatc ttcacccgaa ccttcggaaa
     9481 tgcgtcccca acgcggatcg cgtgagatat ccaccatggg cgaaaatgtc cagttgatac
     9541 cgtcggcagt ggcttcctgg gcagctacac gtgccgattg ctcaattaaa tccatatccc
     9601 agctcgaagc cagaccgagc ggaataggaa atgtggattc gtaaccgtga atgacatcca
     9661 tgccgaaaag catcggaatt ttcatgcggc tttcttctat cgcaacccgt tgcacttcgc
     9721 gaattttttc tacaccttta atattaaata atcctccaac ttttccttct tttattgact
     9781 gtccgatatt tgaacttttt gcctgtccgg tagtgaaatc gcctgccgaa gggagattca
     9841 gctgaccaat tttctcatca agtgtcattt tagacatcag atcatcaatg aattgatcca
     9901 tcaccggatc tgatttgaaa tgaatggact tggttgaaat tggttttgcc ggaacaagcg
     9961 gaatggcagc aatagtctgt tttttcttag atttcttttg tgcagacacc tcctggtaag
    10021 gtataaaaca aaaagtaaag ataataatca gcaataagaa ttttgatgga tttttcatgg
    10081 tttctttgat ttttatgtta caaatttgaa gataatgaat ttacatacca aatttatgaa
    10141 ccctataaga atactacaag caaaaaatgc ttaaaatagg ggttcctatt tttctcctac
    10201 aatacaataa cggttaaatc tataactttg atattcagta aattgtatag caccttgttt
    10261 taaaaaatca catacaatgt caactttttg tatcactcat gatatgaggt gtggcagaag
    10321 taataaaact acttttattt cagataaaac agcagattgc tttgattaat gcctgttcaa
    10381 tgtattgatt ttcaacagat aggatttatt tatcaatatt ttataaatca ttgcctgtgt
    10441 tttgtattca atctatttgg attacaaacc gatagtttac ttcggaattt acctgaattc
    10501 gattaaaaac tcactgatgt ttttttcgga aggaatgttg attttctttc tgatacgata
    10561 gcgggctacc tccactccct tggcagaaat gttcatcaga tgtgcaacgt ctttggtgga
    10621 tagattcaaa cgaaagtaag cacagaaacg taaatcgttg ggtgtcagtt caggatattt
    10681 ggtacgcaga ttccgaaaga aattttcgtg aatacggtca aaattagttt gaaatatttt
    10741 ccagtcgtcg tcagaagata tgctttgatc catcaatttt attattcgct cataatattt
    10801 attcggaaac tgagttccaa gatgcttttt cagttctatc agttcatctt tgatgtttat
    10861 cagtacttca tttttccgga taacggttat agttgacatg gccagttcct tgctttttaa
    10921 tgtaaggtct gattccagct tttcagcttt taacgcaaca atctgcattt cgcgtttttc
    10981 gatttctttc ttgcgtatat tttcctgttc ctgctgtatt cgttgttttt tctgttcaaa
    11041 attacgtttc agaaggttgt aaatgataaa tcctgtcagt atcagcagca atacatagat
    11101 tatcttactg aatgtattcc agtaatatgg cgaacgaacc cgaaacaaat agtctctggt
    11161 tgcaaacaca tctccgttgt tggacactac ccgtacgctg aaggtgtatt ttccgggtct
    11221 caggtaggag tattctttca tattgctggt agtgctttca ctccagttat tattcagtcc
    11281 ggtaagttta taactgaata agagatcgtt gagatttgaa tattgcggat aaccgatagt
    11341 gaaacgaatt ctgttttttg tataggggaa agaaatcttt tcgttagcag aacttaccgg
    11401 caacagcatc atttcttctg aattcttatc caccacctgt acctgcatca gcagaatttc
    11461 tttttttgaa tgataaagcg attgcttccg aagtttcaga ttgtacattg ccagaccgtt
    11521 ttcaagggta aacatggcaa tatcctccgt gaggggaatt atattctgaa aattatctac
    11581 cgtctgattc ataaacaggg agtactgtac tatatcaagt atttgagcac gattgtcgat
    11641 gaattgaatc aatgcagctt ctttttcaag aatgaaccaa taagttgagg gcttttgaat
    11701 acaaatttta taagcccggg catatttacc gagtgtagta ttcagttcat caaagggcag
    11761 tatcttgttc tttatatcat caaatacata gaaacgggtg ttatccgtaa atactaccct
    11821 gttttggaac atgtaggtgt aaatgagtga tgatttttta ttgtcgagcg aggtgaattt
    11881 ctgtatttta tcaatggttt tgaggtccga cttcagcata atcatataaa gtccttcgtg
    11941 catgtgcgaa gcccagattc tgccctgata atcgatagtg atggatttca ccggattaag
    12001 aaaaccctga atgacactgt gaaatttcca ttcattattt tctttcagat atacacacaa
    12061 atcggagtaa gtaccttcta caagtacttc tctaccgtga attattcctc tgccaataca
    12121 cattcctcct ttggaagtgg atagcatctt ccggtcggga taaatacggt aggtttcttc
    12181 attatttccg caaaaaagct gattatcgac atttgtgagt gaccatacct gacctttaat
    12241 ctgggaatcg agctgtatgt tggtcagcga tttggaatca ggtgaaaatc ttccggaata
    12301 aagtccctga ttggtagcca gatacaactt gtcgggaggc agataactca acgagtaaat
    12361 cgaaccaatg gaaggtgtaa atgactgaac aaactgaacg ggactgttga gccttaccat
    12421 ggcaatacct ttatcaaggg caagccacag gttgttttcg gagtcggtgc tcatacccag
    12481 caccgtgttg ttctgcagat tgttggatgt attcagtttc catacctttt tcccgttttt
    12541 gtcaatagca ctgactccat ccagtatggt gcctatcaca agcaatgaat cgcgggtaag
    12601 tgtagcttta tttatttcgg catttcggag gtagtccagg ctgccatcgg agaatttcct
    12661 gaaatcctga ccatcgaaaa tataaatgcc atcggaaaga gtgattacat acgcttcatt
    12721 tttattaaat tgtatgatgg aaattactgc actgctgaaa ggtgtatttt caattttcgt
    12781 gaatttttta tttttcggat caaatgacga taaaccattc tgaagagtat gcacatacaa
    12841 tttattcctg aaacgctgta aataaaggaa agtctcatcg tacgattttc cttccacatt
    12901 tttcccgtca tagatgaaaa tacttttaaa ggactggaaa agaattttcc cgtcaaattc
    12961 aattattttc caaatttcat cattttcaag cttataattt ttgaggtcat tcgaaagtga
    13021 tgaatagact aattctccga atttatcctt ctcaaaatac ccgaactctc tgaacgaacc
    13081 aacataaatc cggtcggact tatcgatata aaccgaacga accacctgat tatttggtag
    13141 tttgaacaac gtgaatccgg aaccgtcgaa ccggagcaaa cctacgttat ttccaaagta
    13201 aattaatccc tgactatcct gtgctacaga ccagttctga ttgcctgcat tgtaatcttt
    13261 cttgttgaac tgggtaacaa cgggtgtaaa atcattggct gcaagcaaaa aactgctata
    13321 tagaaaaaag aaaataatcc gaaccttcaa taatttcata aatccgattt tgaagtatga
    13381 attaatcatg taatttatgt aaattaaaat acattttcac acaaagatag aaaaaaatga
    13441 ttgtgataaa aattttaatg aattacattt tttctgatat ttgacgtgag agcgtattca
    13501 gaaaatatca taccctattc atccgggtaa tttcagggaa aaaatcagaa aatatctgac
    13561 attctggttt ccggacataa aaaaaagcca ctgataaagt tatcaatggc ttttaaacta
    13621 atacagtcag tttttcaact ttctttcttc ttccagagtt tattcatttc ttccagggtt
    13681 ttgccctttg tttcgggcac cattttccag atgaaaatgg ctgccagaat acccatgagg
    13741 ccatataccc aataagaaaa tccatgattg aactggctga ccagcgcttc atttttgtta
    13801 agcatcggga atgtccacga taccaggaag ttggataccc actgtgcagc cacggcaatt
    13861 gacatcacac tcgaacgcac cgaattggga aaaatctcgg ccagcattac ccagcaaacc
    13921 ggaccccacg acatggcgaa acctgccgta tagagcagca tgaaaaacaa cttcaatccg
    13981 cccgaccagc ttccggcaaa tccggtaccc agtccggtca tagccaaagc cattaccagc
    14041 gcaccgatta tgagcagcgg acgtcggccg aacttatcga ccgtaagaat ggccagaaca
    14101 gtaaatgaca ggttgataat gcccacaatg atagtttgca acaaagccgt atcagtgccc
    14161 gatcccatcg atttgaagat ttccggtgca taatagagca ctacgttgat acccacaaac
    14221 tgctgaaaag cagagagcaa tactcccaca ataatgattt tgaaaccaaa agagcggatg
    14281 ggtgcaatga gcgaaatcac aaaacttccg atgagcgaaa tctcgaatgc actcgcattt
    14341 cctgcaaatt tcagtccgat gaaggcaatg gtaaagagca gaatccaggt aaccagaaag
    14401 cggtaatacg gtttaagcga ctgagctgca tcctgatcca ggctaagctg aatatcatct
    14461 atttccacat ctgcttcctg agatcccacc agtttcacaa gcactgacct tgctttatct
    14521 tttttcgaat tcattaccag ccagcgtggt gtttcgggta ctgaaaacag gaaaataagg
    14581 aacaatgaag ccggaattat ttcggatgca aacatccagc gccagccgat ggtgttcagc
    14641 cagctgctgt cgccctgaag ggcaatagca taatttacaa aataaaccac cagcattccg
    14701 aaaatgatgg caaactgatt ccaggaaacc aaagcgccac gtttgttgtg tggtgctacc
    14761 tcagctatgt acataggcga taccatcgaa gctattccca ctcccactcc accaataata
    14821 cgatagaata taaacaaatt ggcaaaagtg tggtctcccg ttccgggcat tccgaaaaac
    14881 atttcgggcc aggcagaccc cacagctgag agtaaaaaca gcacagcagc tatctgtagc
    14941 gagggtttac gtccgaattt ctgactgaca taacctgcaa tggaagctcc tatgacacag
    15001 ccgataagtg cactgctcac aataaaacct tccatggcat ttgccagatc gggcgagagt
    15061 cccagcggat ctacaaaaaa tatccgcagg gaactcactg tacccgagat aacggccgta
    15121 tcatatccga acagcaaacc gcccagcgta gctacaagcg taatacctgc gaggtaaaag
    15181 ggattttttt tattgccgga catgctgtat attttaaatg tacatgttga taatcgactc
    15241 gtacaattcc tgttttccgc taatttgagc aggttcttcg tttttggctt tagcgtaagc
    15301 gtacacatct tcgagtgtga gtttgccatc ttcaaaatcc ttgccgattc cgccatcgaa
    15361 cgaagcataa cgttctttca gcatgcctgg catgggcgat tcttcgagaa ttcctgctgc
    15421 agcttcgagt gcacgtgcaa atgcatccat tcctgcaatg tgtgcaataa aaatatcttc
    15481 caggtcggtg gagttacgac gggtttttgc atcgaagttg gttccgccgc cctgcaatcc
    15541 gccagccttg agcactacaa gcatagcctg ggtcaattcg tacacatcaa tggggaactg
    15601 atcggtatcc cagccgtttt gagcatcgcc gcggttagca tccatcgaac caagcatacc
    15661 ggcatcggca gcgcattgca ggtcatgttc gaatgtatgt ccggcaaggg ttgcatggtt
    15721 tacttccacg tttagtttga aatccttatc gaggccgtgt gcgcgaagga atccgattac
    15781 agtttcagca tccacatcgt actgatgttt ggttggttcc attggtttgg gttcaatgaa
    15841 gaatgtgcct ttgaatcctt taccacgggc atagtcgcga gccattttca gcatttgtgc
    15901 caggtgttcc ttttcgcgtt tcatgtcggt gttgagcagg ctcatgtacc cttcgcgacc
    15961 gccccagaac acataatttt cgcctcccag ttcaatggtt gcatcgagcg catttttaat
    16021 ctgtacagca gcccgcgata cactgtcaaa attcggattg gtagcagcgc cgttcatata
    16081 ccgtttgtgg ctgaatacat tggctgttcc ccaaagcagt ttgatgccag tctgcgccat
    16141 tttctccttg gcatatgcca caatggcttt caggttggct tcgtactctt caatagaatt
    16201 tccttcgtcc accaaatcaa tatcgtggaa gcagaagtac tctataccga ttttctgcat
    16261 aaattcaaaa ccggcatcca ttttttcgcg ggcagcctgc agagcatcgt ccgctttgtt
    16321 ccattcaaaa cttttggttt cgcctccaaa cggatctgaa gattcgctgc acatcgtgtg
    16381 ccaccaggcc attgagaatt taaaccagtc tttcattttc cgaccataaa ccactttttc
    16441 agcatcgtag tagcggaatg cgagcggatt tttactttct tttccttcga atctgatttt
    16501 ttccacagtt gggaaatacg tttttgttgt cataattttt ctgatttttt attaaaatga
    16561 ttatttaaag taatttttta ttatttcaat ctactgatcc acaggctata agcatcctga
    16621 gcagcttcca catctttctc ttcaggagtc actacaagta cttttttcag tgtcgaaaat
    16681 gcctcttcgg ccgactggta aattccggct cctattccgg ctccacgtgc ggcaccgata
    16741 gatccatcgg tattgtacag ttcaatcgtt gcacctgtaa tattggataa tgtctggcgg
    16801 aatatcggac tgagaaacag gttggcatgt ccggcgcgta tggttttggt ttcaattccc
    16861 atcttattca taatgtccat tccgtatttg aacgaaaatg cgatgccttc gtgagctgcg
    16921 cggattaagt gagattttcc gtgcaccaaa aaattgatgc catggatgga gcagtttggt
    16981 tcttcgttgt tcagcacccg ttcagcaccg tttccaaatg gaataatgga aattccttcg
    17041 cttccaactg gaacctttgc agctaaatca ttcatttcgg catatgaaat accttcggga
    17101 gccacgttat tttttatcca ggtgttgaga atggctgttc cgttgataca cagaagcact
    17161 ccgatgcggg ttgcttcaga acagtgattg acgtgagcaa atgaattcac gcgcgattgc
    17221 ggatcgtatt ttgcatcgtc gtttactccg tacaccactc ccgaagttcc tgccgtagct
    17281 gccacttcgc ccggattgag cacgttgagt gagagtgcgt tattgggttg atcgccggca
    17341 cggtaagtga tcggaattcc ttcggacaga cctaattcag ccgcaatttc agctgaaacc
    17401 cgaccttgat gaccaaaagt tggcacaatt tcaggaataa attcttcgga gaatccgtaa
    17461 tgttcataaa ggaaaccggc cggacgattg ttaaggaaat cccacgaaat tccttctgac
    17521 agccctgaag cggtagtagc ggggtggccg gacatgcgaa gtgcaatgta atcgccgggc
    17581 aacatgtagt attttatccg ggagtaattt tcgggctcat tttctttgac ccaagccagt
    17641 tttgacaggg tgaaatttcc gggagaattg agcaggtgcg acaagcattt ttcacttccg
    17701 atgacattga atgccttttc gccataagct atcgagcggc tgtcgcacca gatgatggaa
    17761 ggacggatgg cctgtagatt ttcatccacc agcaccagcc cgtgcatctg atatgaaatg
    17821 ccgatggcat ctatttcttt cggatcgatg ccggcggttg caagcaccga tctggtggct
    17881 tctttgaggt tgctccacca tacttccaca tcctgttcgg cccagccggc tttcaccgct
    17941 tttatttcca tctctttttt cgggaaaaaa tcgctcgcca tcacttcgcc gcttttcgcg
    18001 tttaccagac tggctttcac cgacgaactt cctaaatcgt atcctaataa atacatggta
    18061 gttttttttt gtaaggttat ttattttaat gattatacgt aaagtgtcct tataaaatta
    18121 tttttttgcc tgaaaggcat atttgtttca gcccaatggc aacgccttgg gttaataaat
    18181 tgaaagatca taacggcgcc ctgtaagggc agtttaacag ataagttggt tttggctaca
    18241 ccgatacttt gtattcagcc cgccatatcc tggttttatc atttccccca cccgttggat
    18301 ggggttgaaa taagcagggc tttcagcccg aaatacaaga ttaggtcact atgcggataa
    18361 ccatatttat ttttgtctta ttttttttga tatttataga gtcttccggc acggtgaggt
    18421 acctctttct cgaattcatc gagcacgacc aggttgtgcc actgattgat ttttttctga
    18481 aaattccgta catccgattt tgtccggttt atttcatcgt acaacaggcg gagttgcgaa
    18541 agtgtgaact tgtgcggcag caatttgaac atcagatgag gctccacagt gagccaatgt
    18601 ttgatgtggt caagtccttt ctgtacaatc agattgtggt ccatagccag attcatgttt
    18661 tgcacttcat ccacatcgta ccattcggca gaagtgtttt ttgagttcag ggtgattttt
    18721 ctgttgattt ttatcagggc aacgtagcca atagtgacta ttcgttcgat tttggttttc
    18781 atcgtacgtt cgagccagag aatgtcgcgg ggattgctgg tacgcagcgg attgccaaaa
    18841 ctatagaact gactgagagg gatatttttc agaccggtaa gctctgcaag tacccgtgtg
    18901 gctgcctcat cgagatcttc gtccatataa ataatgcttc ccggcagctt cacatcgttg
    18961 tactctccgg tattctcatc aatatgccgc ttaatgagca acactttcag gtttttgccg
    19021 tcaaacccga atacaacaca gtcaatggaa acgtagggat tgtatggtct cgtttcgctc
    19081 ataaaataat gatctttttt gaaaatattt ttacaaaaat agataaatgt tttcaattac
    19141 aaaattattt acaatacttt taaatgcata ttatataaat atctgtattt tgtacttttt
    19201 acaataagca acaagttgat tattgtatat aatacaatat ttaaaacaga attaaaaaac
    19261 gaagagtccg aaattaaaaa aaacatgcat ttatttattt ttgcattctt tctgctaaat
    19321 atattggtat gaaacaattt tttaaaaacg tgaaaaaagt tgctttcaga cccgtttaag
    19381 gctaaaagcg tgaactattc tgttctttcg attgttttaa cgaaactaaa ggtcagaaac
    19441 agcggtaatt ccaagttttt ttccgacctt tgcaaaaaat ttatttgaca tatttttaat
    19501 gaaaacaaac aacgaaaaca ggcgtggaag ttatggttcc gacgccagcc gtgatggtaa
    19561 atccttcgac agaaaaaatg caaagccttt attgaaaggc atcagaacaa gctcaccctc
    19621 caattttgga tccagaaacc aggacaacag gtacgacaga accgctaaca acgacagaaa
    19681 ccggttttcc gacagaagtg aaaacagata cgaaagaaaa gatgacagac caaattccta
    19741 ctcggaaaga aaaccgtaca tcaacagaaa taatgacgac agacgctctg aaccgggttc
    19801 ggaaagaaaa tttttcgtaa accggaatac cgacaacaga cgcactgact ccgactccga
    19861 cagaagacac tattccagtc gcaacaacga cgacagacgc aatgattccg gacaggaacg
    19921 caaaccgtgg ttgaacaaag atacgaacag acagagttcg ggccgtcccc gttttatccg
    19981 caagtccgaa gtcggcgata agcctgcaga atattctacc ccgcgtaaac gtttcgatag
    20041 cgacgaaaga ccccgcgatg gaaagctcac tttcagaaaa gccggtgaag tgaaggactc
    20101 caattatgta gaaacccgtt tcgacaacaa gaaaaacggt attactttcc gtaaacgtca
    20161 taattcggcc gattacgatc cgaacgccaa atacagcaag aaaaaacaat tcgaatacag
    20221 acaatcatac attgacccga caaaaccgat tcgactcaac aaatacctgt caaatgcagg
    20281 tatctgctcc agacgcgaag ccgatgaata tattcaggca ggtgtgattt cggtaaatgg
    20341 taatatcgtc accgaactgg gtacgaaagt actccccacg gacaatgtga tgttccataa
    20401 tcaaccggta cgaatcgaaa agaaagtata tattctgctc aacaagccca aggattgtgt
    20461 aaccacttcg gaagatacgc aggcaagaac cacggtgatt gatatagtta aaaatgcatg
    20521 cagcgaacgt atttatcccg taggaagact tgacaggaat actaccggag tactgcttct
    20581 gaccaacgat ggcgatctgg cttcaaaact cacccacccg aaatacgata aaaagaaaat
    20641 ttatcacgta ggactcgaca gagacctgga aagagaagat ttcgaagcaa tactggatgg
    20701 aattgacctg ggagaattca ccatcaaagc cgatgcgctg gagtttgtca aagatggcag
    20761 cctgcgcgaa ataggcatcg aaatccactc cgggcaaaat cgggtggtaa gacgcatttt
    20821 cgacaagctg ggttataaaa ttacaaaact cgaccgtgta ttctttgccg gactgaccaa
    20881 aaagaacgtt ccgcgtggaa aataccgctt cctcactgaa aaggaagtga atatcctgat
    20941 gatgggagcg catgagtagt tttgagtttt gagttttgag ttctattgta aattacaaat
    21001 cagttcattt cgattaattt tatattaata cttaaaaagc ttcgttatgg caactcaaca
    21061 aacctcagac gagcctgtta atcaggtatc gacaacaacg actacaaaaa ggaaaccggg
    21121 caaagtggtg aaatatattt tgaaccacaa gttggcgttt tcgctgcttt ttgccttaat
    21181 cgtggtattt atctgggctc aatggcagat ccataaactt gaaaaagaaa aacaggctct
    21241 taccgaaact tataaaatgc aaatcgacag cctccggcag gcagattata tgcttgtttc
    21301 gaaagtcttc tcctgggcag tacgcagcga tatgatgcgc aacaactacg atcaggcaaa
    21361 ccagtatatc gatttcatca tcagggaacc tcacattctg aaagcgtttg ctattgatgc
    21421 tgccacaaat accatcgtgc tctcgtccga caaaaatgag gtaggacttc ctgtagctga
    21481 tatcacactg ctacggcctg tagaaaacgc aatggtaaga agcgacagca cagtacgatt
    21541 tgtaactccg ataactgcac taaatcaaaa agccggcgtt tcggtactcg tgactaatct
    21601 gaaataattt tttttttaca tagttaataa ataatccaca gggatgcttc gcgctgttac
    21661 cctgtggatt tttttattca tattcctcac caaatcttca ctgctccgtt ttcaaaaata
    21721 atacacataa ccaataccgg caattcagaa atacgaacaa agtgaatact tgctgaagat
    21781 tgattttaag agtactgtt
//
LOCUS       DAORKP010000016        21781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_99_length_21781_cov_8.425113, whole genome shotgun sequence.
ACCESSION   DAORKP010000016 DAORKP010000000
VERSION     DAORKP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99_length_21781_cov_8.425113"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..707)
                     /gene="tsaB"
                     /locus_tag="PLN75_01870"
     CDS             complement(21..707)
                     /gene="tsaB"
                     /locus_tag="PLN75_01870"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRG02894.1"
                     /translation="MTILHIETSTNVCSVALSNDGVCLFEISNNEGLNHAAMLSPFVQ
                     QAIGKMNELHLKPEAIAVSAGPGSYTGLRIGVSTAKGLCYGYKIPLIAVGTLEILAVD
                     ALKTGILNDHALLCPMIDARRMEVYSALYTEKLELKREVSAEIINENSFQEILDQQPV
                     YFFGNGASKCKPLITHPNARFIDSIEPLAKNMILTAEKKYAAHDYADVAYFEPFYLKE
                     FQATIPKQLF"
     gene            complement(788..1459)
                     /locus_tag="PLN75_01875"
     CDS             complement(788..1459)
                     /locus_tag="PLN75_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRG02895.1"
                     /translation="MKQNKNNKSFQFILITFLALLSGTSLQSQTVLLQPAEVYIGTSF
                     GANGSMMMFNPTVRQIPLLGYQGGLAFRYITEKHVGLQIEMNYSQRGWDEESGLYSRR
                     LNYLELPFLTHLYLGNTHRFIFNIGPKVSYLLNETVLINGMENSDAEQHVKPVYFPFD
                     YGLTGGLGYNLHTRRAGVFQLELRAYYGLSDIFANTKSDYFANSNHMNAALSVGWFIQ
                     LTGKE"
     gene            complement(1440..3338)
                     /locus_tag="PLN75_01880"
     CDS             complement(1440..3338)
                     /locus_tag="PLN75_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRG02896.1"
                     /translation="MKYKFYIMLIIGITCFLPTIYTQNESYTTEIINGKEYYRYKVQA
                     SEGLYAISRKFGVSQSEINNINPQIHDGLKAGQTILIPKTTASHSAAPSVKKIADNTE
                     YVTHTVERRQTLFAISRKYNVTQDEIIQANPQIKERGLQPGNVIRIPVNKTTPKTAEV
                     QTPVKTETDGNQAPVKKEEIKTMQTVKPAEKVLHIGSEGSYITHVVEPKETFYSISKK
                     YNVTVQEIKDLNPESGDVLKTGMTLKIPFKSGSATSSKTTAPETSVPEPKTTSTTTST
                     TQPTVIQQPKQTLSKDTYKIAFLLPFMLSGNQNDPTVEKFVEFYMGSLLAINNAKNGD
                     MNYEIFTYDIEKTETMVYEVINKPEMQEMDLIIGPAYTAQTAVLADFAKRHKINTVIP
                     FSSKISYIDSNPYIFQFNPDQELQNEFTLNLLKNRFEGANLLFVETGNVKMSDDGLDF
                     FNFLMKKLDKQKVEYKKISGSDADNLQNYLFTDKYNLIIFDTEDEKSVQTYLNKLYDL
                     HTKYNLGVLGQYAWRNTKSKKPHMFYISPFAGNKTATDFYEQEYQKYYGKMRSGTNPR
                     FDLLGYDLTTFFLSTMKNDGFTFNRTTPSLTFDNGVQSDLHFVKTGKNGGYVNQKLYL
                     IEDEAKQK"
     gene            3540..4805
                     /locus_tag="PLN75_01885"
     CDS             3540..4805
                     /locus_tag="PLN75_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRG02897.1"
                     /translation="MKRIILSVIMIISVVASFAQLYAVGQYAEVSLSGDVNHIYLFGS
                     IDDQTEIHYKSSNPTVYIQWFTYLNGTRTPLTSVSTTSSPSTIDTYFKPQNNTGYIVS
                     VDGKETTFWVYDYSRYLFTSNSFGVSDADSPCEEVSVSLQGVIPEFTYQSPTGRIVLL
                     PRSYELKYTTLSWNGENWENKDTTLNISVPPLHFQVPVPLANTAFTLTGDEYARKMNL
                     QPGVIISDEYKTNAVKCKITTLTAARSELNENERPDTTTLSGSAPLEIQFFGNPTPNV
                     RNFLWKIYKDGNLMLTRTTQDHQYNFTESGNYKVKLQVSNNFCTDSAFVDVIISESQI
                     VAPKIFTPNGDGFNDEFRVAYKSIIEFNATIINRWGRVLFKWTDPAKGWDGKINGKPA
                     AEGTYFYIITAKGSDGKIYPLKGHINLLR"
     gene            5064..5504
                     /locus_tag="PLN75_01890"
     CDS             5064..5504
                     /locus_tag="PLN75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRG02898.1"
                     /translation="MTIRQLSVFMENKSGILNEILSVLAQNNINIAALTVADTNDYGI
                     LRLLVSDPQSALEKLREKQFTVRIHDVLSLEMAPEPGSLYRILHHFAEARISMEYVYA
                     FSFGSKSIVVFRTDNREKAEKVIKENGLKSVREEDLKPTQTLSE"
     gene            5608..6900
                     /locus_tag="PLN75_01895"
     CDS             5608..6900
                     /locus_tag="PLN75_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRG02899.1"
                     /translation="MWNKEIECITRQKMRELQSERLRELVQRVYNNVPFYQKKMDNAG
                     VKPTDIKSIDDISKLPFTSKQDLRDNYPFGLFAVPMKDIVRVHASSGTTGKPTTVGYT
                     RNDIEIWKEVVARCFTMCGIGKDDIMQVAYGYGLFTGGLGAHYGAEEVGASVIPISGG
                     NTRRQLQLMSDYGVTALACTPSYALHLADAMAEHGYSLKDMKVKKGVFGAEPWTENMR
                     LELERKWGIHAFDIYGLSEVMGPGVSNDCEFHTGLHVNEDHFFPEIVHPDTKQPVAAG
                     EMGELVFTTLTKEGIPLIRYNTRDLSSLNFEKCECGRTLVRMKKITGRSDDMLIIRGV
                     NLFPSQIEHVLLEMGETSAHYMLYVDRHNNLDTLELQVELDESKLTDTIKDLQILSRK
                     IEHALNSAIGLSVKVTLVEPKTIARSEGKAVRVVDRRK"
     gene            6985..7566
                     /locus_tag="PLN75_01900"
     CDS             6985..7566
                     /locus_tag="PLN75_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02900.1"
                     /translation="MKYRIILLAFTLLTVTGCVNLRSGIFVENDRVMKTRKVTYETYF
                     VSNQRYEQSYTQKITFLKETDRNSITNYTLYDVITLPVESFDLDDKMYIVADDNVIKL
                     PVSTQNQYNTKNINEQKNEILQSDSTKITVVTGYDVVNKKTIQMTHPINDDILQKILQ
                     AHELHLRYYVGPNAINSEIKGTKLRNVKKWILK"
     gene            7571..8866
                     /locus_tag="PLN75_01905"
     CDS             7571..8866
                     /locus_tag="PLN75_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRG02901.1"
                     /translation="MIPKQLVDGLFSQFGTEDINQLSIRQVGKLVAQIEQQSGVEFIH
                     MEMGVPGLPASHIGIEAEKEALDKGLASIYPNIEGIPFAKEEIARFVRSFMNIETEPT
                     YCIPTVGGMQGSFAALLTAARTNAAKPYTLFIDPGFPVQKSQLNLLGLPFKAFDFQHF
                     RGEKLRDKLEEFLKNEDICSICYSSPNNPTWCCFSEEELKTIGELAEKYDVIVIEDLA
                     YFGMDFREHYGIPGEAPFQPTVARYTHNYILILSASKIFSYAGQRIAMLVFSSQAYNR
                     SYPNLRKYFPADTMGRFIPYGVLYATTAGTSHSAQYALATMLKAANDAKINFVELLHH
                     YGEKARKMKEIFLKHGFYIVYNDDERELADGFYFTIAYPGLTSGELMKKFLYFGISAI
                     SLAITGSENPNGLRACVSFVRMDQMEELERRLEEFVHSV"
     gene            9246..12761
                     /locus_tag="PLN75_01910"
     CDS             9246..12761
                     /locus_tag="PLN75_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HRG02902.1"
                     /translation="MENIKTSEVIDWASNFMSRALNEIREVERKEQNKYAALVQNPND
                     KVLMTKMLDETSQIRDNRKLAKRIRLLLKEYGTPEFFNAGERLMLTAFNSVGYLVPGI
                     SVPIFKKYLRDYTSAIIINERPDLLNRHLDERNKQKIGQNVNLLGEVVLGDGEARKRY
                     EHYLEALKDPRINYISVKLSGIYAQINPLNYDTCKNELCTLMGNLYQQSMDFPFKDEK
                     GELSYKFVNLDMEEYKDAELTLEVFKTVLSEPRFKNLTAGIVIQAYLPDALKLQGELL
                     EFGKKRFAEGGAPIKMRLVKGANLAMETIVSSLRGWENPIYDNKIKVDANYLKVLDVA
                     LLPENAKAVHVGVASHNFFTIGYAYLLAKKLQVDEFVTFEMLEGMANHLPRVMRSLDK
                     QIILYTPVVNDEHFLNAVSYLVRRMDENTGKDNFLTYSFNLKEGSKEWDFLKKQFEDA
                     MAIKDTLETVPKRTQNRNLQQPALIQDVFKNEPDTDFDLRENRLWVNQIRKKWMKSEK
                     DEPYQIPAQVGEKEYTTNHKKKYFDRSQDSKVLVCEVSTVSAEQMKEVISVAQTDKSG
                     WRKTTLAKRNKLLQQSATNLANKRGDLIGCMAAVTGKTFNEGDVEVSEAIDFCRFYPI
                     SMKKFDELKTVKNSPKGIVLVIPPWNFPTAIPVGGVVAGLATGNTVILKPATVAFPVA
                     WEFAQCFWEAGVPKDALQVICPDGSEALNYLTSNSSISHIIMTGGTDTAFKLLENNPS
                     CPLSAETGGKDAIILTASGDRDHAILNVVKSAFGNAGQKCSACSMLVVEKSVYDDPDF
                     KAKLKDATESMHTGSAWDGGNLVGPMVTNTNDKLLHAIDNPEDGESWLVAPQFLDKDR
                     YILKPCVKWGVKPGSYTFENELFGPLLAVVCMDDLKHGIEIVNSSEYGLTSGLQSLDE
                     SERKLWKDTLEAGNLYINRGITGAIVQRQPFGGMKRSAFGGGIKAGSENYVTCFVNFE
                     ETGVTDSSSSNSTGFNTLAEMLPADERSRFVSAVESYTQNWKNEFSVERVTQDLIGEL
                     NTFRYLPIKNIVLRMLPEDNLCDVLMCVAAARIAHTPLVISAPGDDKRVKEFRRLSAQ
                     EVALIVQSDDEFIDKMDDYYRVRCCSDKLPMSYYKKAAKLGKYIATAKPLVEGRLELL
                     HYLREQSISYEYHRYGSFSEEDAKL"
     gene            complement(12838..13572)
                     /gene="ubiE"
                     /locus_tag="PLN75_01915"
     CDS             complement(12838..13572)
                     /gene="ubiE"
                     /locus_tag="PLN75_01915"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRG02903.1"
                     /translation="MNIKEVTPYHTEEDKTTQLYKMFNSISRKYDKFNDIMSMGLAHK
                     WRDKALETLTPYNHNQLLDLACGTADVCIKACYVLNPEHVTGVDISEGMMEVGRMKIN
                     AEKLNEKITLQKEDVSCLSFADETFDAATTSFGIRNFDKLDESIREIHRVLKPLGKFV
                     ILEMSEPKTPVIRQGYLLYTRTVIPFAVKTFSNDPLAYKYLTDSMKVFPQGKEMIAIL
                     KKNGFRCLKYKTFFLGVCSLYVLEKE"
     gene            complement(13742..14644)
                     /gene="lgt"
                     /locus_tag="PLN75_01920"
     CDS             complement(13742..14644)
                     /gene="lgt"
                     /locus_tag="PLN75_01920"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRG02904.1"
                     /translation="MLNYITWNVNPELFSIGPLTVRWYGALWALGIFLCLLIVSKLYK
                     HEKLPDTWIDKQFLYVVIGAIVGARLGHCLFYEWHLLAKPVEILGITFKYGNPYLAKP
                     WELLYIWQGGLASHGGAIGIIIAMYYYNKNVTRKGFIWGFDRLVIGVAITGACIRLGN
                     LMNSEIYGSATSLPWGFIFVRDGQTEPMHPTQIYEMLYCLVTFAVTWWMYWKKQAYKK
                     IGMIFGVFLLGIFGTRFLLEFIKLNQEAFESGMWLNMGQILSIPFIVWGLYLIFFYSK
                     NHIQPDEIPVETQKVRQPKPKKQK"
     gene            complement(14797..18108)
                     /gene="secA"
                     /locus_tag="PLN75_01925"
     CDS             complement(14797..18108)
                     /gene="secA"
                     /locus_tag="PLN75_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRG02905.1"
                     /translation="MGLNDFLKKIMGNKAQRDMREISPEVEKIKAAYENIKSLSNDEL
                     RARTETLKNKIQDSIAPDNARIAELKASIEDTELHLREKIYNEVDKIEKDITEKIEKV
                     LIEILPEAFAIMKDTARRLNENAEVVVSATDFDRNLAAKYDFVRIEGDKAIFKNEWMA
                     GGNLIKWDMVHYDVQLFGGVVLHSGKIAEMATGEGKTLVATLPVFLNALTHRGVHVVT
                     VNDYLSKRDSEWMGPLYMFHGLSVDCIDKHRPNSEERRQAYLADITFGTNNEFGFDYL
                     RDNMATSPLDLVQRMHNYAIVDEVDSVLIDDARTPLIISGPIPKGEDQLFDELRPKVE
                     KLVSAQRQLVTKYLADARNLMKSDDEKVRDEGTLALFRSYKGMPKNKPLIKYMSEQGV
                     KTAMLKTEEIYMEQNNRRMPEATDPLYFVIDEKNNSIEMTDKGIDLITGNSDDPLLFV
                     LPNVGDEIAELEKNTTLNKEEKQAKKDEILQNYSVKSERVHTINQLLKAYTLFERDVE
                     YVVIENQVKIVDEQTGRIMEGRRYSDGLHQAIEAKEGVKVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAGELWNIYKLDVVVIPTNRPIARNDMEDRVYRTKREKYQAVIEE
                     VVNLVNAGRPVLVGTTSVEISELLSRMLTGRGIKHNVLNAKLHQREADIVAEAGRKGT
                     VTIATNMAGRGTDIKLTPEVKTAGGLAIVGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     FVSLEDDLMRLFGSERISGMMDRMGFKEGEVIEHSMISKSIERAQKKVEENNFGIRKR
                     LLEYDDVMNAQREVIYGKRRHALIGERIGVDISNMIYDGAEAMIEMAKSDEDYDGFNL
                     DLMKVFAMDSPVTEDEFKSTRMKDLTEKVVDEARQTFKRKTEKMASIAYPVIKDIYEN
                     KGDQFESILIPITDGKRVFNITTNLKTAYNNEGREVIKDFEKQTLLYVIDDEWKEHLR
                     QLDELRQSVQNASYEQKDPLLIYKLESFGLFKEMVTSINRKVLSILMRGQIPTRDPKE
                     VRQGEQQRRSDYSRYRTQKDEIASRSSSASNDPTKQDTREQQKPEPIRVEKKVGRNDP
                     CPCGSGKKFKNCHGA"
     gene            complement(18369..19691)
                     /locus_tag="PLN75_01930"
     CDS             complement(18369..19691)
                     /locus_tag="PLN75_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRG02906.1"
                     /translation="MKKLSDWIKYAFKFVSFDIWRITGNELTKTRRITYNIIKTIYLS
                     VRGFTKNKLGIRASALTYSISFAIVPLIALISAIAKGFGIEAIIETALQDTFAAQANL
                     IPTIMEFVRKYLDSMSGGLFIGIGLGILVYSVYNLFAQIETALNSIWQVQKSRSVLKQ
                     FTLYFSGLLIFPILMAISSGLSIYVNTILKESFLFQIFTPIMKFIISTIPYLTSGLLF
                     TLIYLIVPNTKVRFLNALIAGMITGIVFQAFQGLYVSGQINLTRYNAIYGGFAAIPLL
                     LIWLNISCLIFLIGAEISYVSQNLRHFDYEVDTDNISNRYKRSLTMFVTYVIVKRFQA
                     NEPPLTQEQIVSQYKLPIRLLNTILTDLTESQIIASTESRKGVKAYLPAIDINQLTLK
                     TMEMKLDMKGSELFLESKNEEMDAFWQKMIGIQEKSDLIHEKILLKDL"
     gene            complement(19779..20197)
                     /gene="rnpB"
                     /locus_tag="PLN75_01935"
     ncRNA           complement(19779..20197)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLN75_01935"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            20414..20746
                     /locus_tag="PLN75_01940"
     CDS             20414..20746
                     /locus_tag="PLN75_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02907.1"
                     /translation="MKTEEDDLLIYDDDDAVKFIVNLVTGEMKDRITEDNVNYVLDVL
                     YDFYDEKGYMGDDIAEEASIDEEEMLKYVVKAAKKDGFDLTEDEIQFVLDGEYEYGKS
                     LGIYTDEE"
     gene            20821..21702
                     /locus_tag="PLN75_01945"
     CDS             20821..21702
                     /locus_tag="PLN75_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRG02908.1"
                     /translation="MTYYIVYGFVKLLSLLPFAVLYALSDVLYPVIYYIIGYRKQVVR
                     DNLSYSFPEKTEQERKAIEKKFYRHFCDLIFENIKFAGISVSEINKRMLYNNFEPALK
                     HLDEGRSVMLYSSHYGNWEWNSSFILHLPKDMPIYPIYKKQTSEVSDRIIHKIRNHFG
                     TETIEMKNLLRVMAAMRSEGRFGMFGMISDQSPTRESLHYFTQFLNQHTAVITGTEQI
                     ARKFDYPVYYARLKKLKRGYYTSELIPVSLHPKETPEFEISEKYMRLLEEDILRDPAI
                     WLWTHKRWKYTREKTVE"
BASE COUNT         6281 a         4403 c         4615 g         6482 t
ORIGIN      
        1 aaccagtatt ttttgttttt tcaaaaaagt tgtttcggta tagtagcctg aaattccttg
       61 aggtagaaag gctcaaagta agctacatca gcatagtcat gtgcagcata ttttttttcg
      121 gccgtcaaaa tcatgttttt tgccaacggc tctatggagt caataaaccg ggcattcgga
      181 tgagtaatca gcggtttgca ttttgatgca ccattgccaa aaaaatatac cggctgctga
      241 tccaggattt cctgaaatga gttttcattg attatttcag ctgagacttc cctttttaat
      301 tccagttttt cagtatataa agctgagtaa acttccattc ggcgggcgtc aatcatgggg
      361 caaagcaatg catgatcatt cagaatgcct gttttcaaag catcaactgc cagaatttca
      421 agcgtaccca ccgcaatgag cggaattttg tatccgtaac aaagcccttt ggcggtagat
      481 actccgatgc gtaacccagt ataagaaccc ggtccggcac ttacagctat tgcctccggc
      541 ttcaggtgca actcattcat tttgccaatg gcttgctgta caaaagggct gagcatggct
      601 gcatgattca gtccttcgtt atttgaaatt tcaaaaagac acactccatc gttgctgagt
      661 gctacagaac aaacgttggt agaagtttcg atatgtaata ttgtcatatt tctgatgttg
      721 taataaaaaa ctgactgtca aagattggat ttacagcagc atttttttta aaaaaattaa
      781 tgattgatta ttcctttccg gtaagctgga taaaccatcc gacgctgagt gctgcgttca
      841 tgtgattgga attagcaaaa taatcacttt ttgtattagc aaaaatatca ctcaaaccat
      901 aatatgcccg gagttcgagc tgaaaaacgc ctgctctgcg ggtgtgcaga ttatagccca
      961 atccgcctgt aagaccgtaa tcaaatggaa aatagaccgg ttttacatgt tgttctgcat
     1021 cagaattttc catgccgtta atgagcactg tttcgttgag cagatagctt actttgggcc
     1081 cgatattaaa tataaaacgg tgagtgttac ccagatacaa atgtgtaaga aaagggagtt
     1141 cgaggtagtt gagcctgcgc gagtaaagtc cgctttcttc gtcccatccg cgctgcgaat
     1201 agttcatttc aatttgtaaa ccaacgtgct tctcagtaat atatctgaaa gcaaggccac
     1261 cctgatagcc caacagggga atttgtctta ccgttggatt gaacatcatc attgaaccgt
     1321 tagcaccaaa cgatgttccg atataaactt ctgccggttg gagcagtacg gtttgcgact
     1381 gcagcgaagt gccggataat aatgccagga atgttattaa aatgaactgg aatgatttat
     1441 tatttttgtt ttgcttcatc ttctatcagg tacagttttt gatttacata gccgccgttt
     1501 tttccggttt tcacaaaatg gaggtcggac tgaacaccgt tgtcgaatgt tagtgatggc
     1561 gtggtgcggt tgaatgtaaa gccatcgttc ttcatcgtgg aaagaaagaa agtggtgagg
     1621 tcgtatccca gcaaatcaaa gcggggattg gtacccgaac gcattttacc gtaatatttc
     1681 tggtattcct gctcgtaaaa atcggtggca gttttgttgc cggcaaatgg tgaaatataa
     1741 aacatatgcg gttttttgct tttcgtgttt cgccaggcat actgaccgag aactcccagg
     1801 ttatactttg tatgcaaatc atacagttta ttcaggtagg tctgaactga tttttcgtct
     1861 tctgtgtcga aaataatcag gttgtatttg tcggtgaaaa ggtaattctg gaggttatct
     1921 gcatctgaac cggatatttt tttatactca actttttgtt tgtcgagttt tttcatcagg
     1981 aaattgaaaa aatccaatcc atcatccgac atttttacgt ttccggtttc tacaaaaagc
     2041 agattggctc cttcgaaccg gttttttaat aaattcagcg tgaattcatt ctgtaattcc
     2101 tgatccggat taaactggaa aatatacgga ttggaatcga tgtatgaaat ttttgaactg
     2161 aaaggtatga cggtattgat tttatgacgt ttggcaaaat ctgccaaaac tgctgtttga
     2221 gccgtatagg ccggtccgat gatcaggtcc atttcctgca tttcgggttt gttaattact
     2281 tcataaacca ttgtttcggt tttttcgata tcataggtga aaatttcata attcatatca
     2341 ccattttttg cattattgat ggctagcaaa gatcccatgt aaaattccac aaatttttcg
     2401 acagttgggt cattctgatt ccctgaaagc ataaaaggca acagaaaggc tattttgtac
     2461 gtatctttac tcagtgtttg ttttggttgt tgaattaccg ttggctgagt ggtggatgtt
     2521 gtggttgatg tagttttcgg ttccggaaca gatgtttcgg gagcagttgt ttttgaactt
     2581 gttgcagatc cggatttgaa gggaattttc agcgtcattc cggttttcag cacgtctccc
     2641 gattcgggat tcagatcctt aatttcctgc acagtgacat tatatttctt gctgatggaa
     2701 taaaaagttt ctttcggctc aaccacatgc gtgatgtatg aaccttccga cccgatatga
     2761 agaacttttt ctgccggttt aaccgtttgc atggttttta tttcttcttt ttttacaggt
     2821 gcctggtttc cgtcggtttc ggtcttcacc ggagtctgca cttctgccgt ttttggtgtg
     2881 gttttattca caggtatccg aatcacatta ccgggctgga gtccgcgctc ctttatctgt
     2941 ggatttgcct gaattatttc atcctgagtc acattgtatt tacggcttat ggcaaataac
     3001 gtctgtcttc gttcaaccgt atgtgtcaca tattctgtgt tatcagcaat tttctttact
     3061 gatggtgcgg cagagtgact ggcagtagtt ttcgggatca gaattgtttg tccggccttc
     3121 aatccgtcgt gtatttgagg gttgatgttg ttgatttcag attgagaaac accaaatttc
     3181 ctgctgatgg catacagacc ttcggatgct tgcactttat atctgtagta ttctttgccg
     3241 ttaataattt cagtggtgta ggattcattc tgcgtgtaaa tagttggcaa aaaacaggtt
     3301 atgccaatta tcagcatgat ataaaactta tacttcatgt cttgaaatta taattaatgt
     3361 tttttaaaaa tcaaggtttc cgaaaaaaac gatttacaaa tgtacgaaaa tttacacatc
     3421 ataatgaata atcaacaata aataacgctt ttttttgttt atagaagagt aatttcacag
     3481 ttttaaggga attggttttc ccgggtgttt tattgctgat aatgaaaaca ataacctgta
     3541 tgaagcgcat tattttatct gtaatcatga taatttcagt cgttgcatct tttgcacagt
     3601 tgtatgctgt gggtcagtat gctgaagttt cgctttcggg cgatgtcaat catatttacc
     3661 tcttcggttc catcgacgac caaaccgaaa tacattacaa aagcagtaac ccgactgttt
     3721 acattcaatg gtttacttat ctgaatggaa caagaacacc actgacatcc gtttcaacca
     3781 cttcttcgcc ttcaactatt gatacatatt tcaaacctca gaacaacacc ggatacatag
     3841 tgagtgtgga tgggaaagaa accacatttt gggtatatga ttacagccgg tatttattta
     3901 cttctaattc gttcggcgtt tcagatgctg attcaccctg cgaagaagta agtgtgagct
     3961 tgcagggagt aattcctgaa tttacctatc aaagtcctac cggaagaatt gtgttattac
     4021 cacggtctta tgagctgaaa tacactacat tgagctggaa tggcgaaaac tgggagaata
     4081 aagataccac actgaatata tcggttccac cgcttcattt tcaggttccc gtaccacttg
     4141 caaatacagc attcacgctt acaggcgatg aatatgcccg gaaaatgaac ttacagcccg
     4201 gagtcatcat ttcggatgaa tataaaacga atgcagtaaa atgtaagata accaccctta
     4261 cggcagcccg ctctgaactg aatgagaatg aacgtcctga tactaccaca ttgagcggtt
     4321 cagcaccact tgaaattcag tttttcggca atccgacacc gaatgtcaga aatttcctct
     4381 ggaaaattta caaagacgga aacctgatgc ttacacgcac tactcaggat catcagtata
     4441 attttaccga atcgggcaat tataaagtca aattgcaggt gagcaacaat ttttgcaccg
     4501 attcagcgtt tgtcgatgtt attatttctg aatctcagat agttgcaccc aaaatattta
     4561 cacccaacgg agatggattc aacgatgaat ttcgggtagc ttacaaatcc atcatcgaat
     4621 ttaatgcaac gattataaat cgatggggtc gtgtgctgtt caaatggacc gatcctgcca
     4681 aaggctggga cgggaaaatc aacggtaaac ctgcagccga gggaacatat ttctacatca
     4741 tcactgccaa aggttccgac ggaaaaatat acccgctcaa aggacatatt aatttactga
     4801 gataaatcag gcttgtcaca ttctcccgat cccgtaaatt gccgaccggg tttacttccg
     4861 aatacattta tcattttttt tcaatatgcc tgaaaaacat accaaagtac ttcagaaaaa
     4921 cattattttc ggcgaaaaat ttagcaaata gttccaaaat tgttaacttt gcatgcaaaa
     4981 cagatttaaa aagtcttgct tggtttttcg aatgagcttt tttatctaaa accatacagc
     5041 aaattcacct aaataactgc accatgacta tccgccaatt atctgtattc atggagaata
     5101 aatcaggtat tctcaacgag atattatcgg tacttgccca aaacaacata aacatagcag
     5161 ccctcacagt agctgatacg aatgattatg gtattttacg cctgcttgtt tccgatcctc
     5221 aatcagcact tgaaaagctg cgggagaaac aatttacagt ccgtattcac gatgtccttt
     5281 cgctggaaat ggctcccgaa ccaggttctc tatatcgaat tttgcatcat tttgccgaag
     5341 cgcgtatcag tatggaatat gtgtatgctt tcagttttgg aagcaaatcc atcgtggtat
     5401 tccgcacgga caatcgggag aaagctgaga aagtaatcaa agagaatggg ttgaaatcgg
     5461 tgagggaaga ggatttgaaa cccacccaaa ccctttcgga gtgagggctt taactcacat
     5521 ttcaagatta tatacaaaac ttcaaaataa caatataaag tctcccttcg gggagcctgc
     5581 ctgccgcagg caggatttag agggactatg tggaacaaag aaatagagtg tataactcgc
     5641 cagaaaatgc gggaattgca gagtgaaaga ttgcgtgaat tagttcaacg tgtgtataat
     5701 aatgtgcctt tttatcaaaa aaaaatggac aatgcaggtg tgaaacctac tgatattaag
     5761 tcgattgatg atatttcaaa attgccattt accagtaaac aggacttgcg cgataattat
     5821 ccttttggac tttttgctgt gccgatgaaa gacattgtgc gcgtacatgc atcgagcggt
     5881 accaccggaa agcctacgac tgttggatat acccgaaatg acatcgaaat ctggaaagaa
     5941 gtggtagcaa ggtgttttac catgtgtgga ataggtaaag atgacattat gcaggtggca
     6001 tacggttacg gactgtttac cggaggactt ggagcacatt atggtgctga agaagttggg
     6061 gcatccgtaa ttcccatttc gggcggaaat acccgccgcc agcttcagct catgtccgat
     6121 tacggtgtca cagcgctggc gtgtacacct tcttatgcgc tgcatctggc cgatgccatg
     6181 gccgaacacg gttattcact caaagacatg aaagtgaaga aaggagtctt tggtgccgaa
     6241 ccctggactg aaaatatgcg actggaactg gaacgaaaat ggggaatcca cgcctttgat
     6301 atctatggac tgagcgaagt aatgggaccc ggagtttcca acgattgtga atttcacacc
     6361 ggactccacg tcaacgaaga tcattttttc ccggaaatag tgcatccgga caccaaacaa
     6421 cctgtagcgg caggcgaaat gggcgagctg gttttcacta cattaaccaa agaaggaatt
     6481 ccattgatac gctataatac acgcgacctg tcgtcattga atttcgaaaa gtgcgaatgc
     6541 ggaaggacac ttgtaaggat gaagaagatt accggacgaa gtgatgatat gctcatcata
     6601 cgtggtgtga atttgttccc atcgcagata gaacatgtac tgctcgaaat gggcgaaaca
     6661 tcggctcact acatgttgta tgtcgatagg cacaacaacc tggatacact cgaactacag
     6721 gtggaacttg atgaaagcaa actgaccgat acaatcaaag accttcagat tctctcaaga
     6781 aaaatagaac atgcgctgaa cagtgcaatc ggattgagtg tgaaagttac gctggtagaa
     6841 ccgaaaacca tagcacgaag cgaaggaaaa gccgtgaggg tagtggatag acggaaatag
     6901 tggaaatttc atttttgtgt tattaatttt ctaaaaagta tttgataatc attttgttaa
     6961 tcaattcaat gatttatttt cataatgaaa tacaggataa tattattagc atttactttg
     7021 cttaccgtta caggttgtgt aaatcttcgc agtggaattt ttgtagaaaa tgaccgggta
     7081 atgaaaaccc gcaaagtaac ttacgaaaca tactttgtat caaatcaaag atatgaacaa
     7141 tcctatacac aaaaaattac ttttctgaaa gaaacggaca gaaatagtat taccaattac
     7201 acattgtatg acgtaatcac acttccggtt gaaagctttg atttggatga taaaatgtat
     7261 atcgttgcag atgataacgt tataaaattg ccggtcagta cacaaaatca gtacaacaca
     7321 aaaaatatca acgaacaaaa gaatgaaatt ttgcaatctg actctacaaa aataactgta
     7381 gttacaggtt atgatgtagt aaacaaaaaa actatccaaa tgactcatcc gataaatgat
     7441 gatatacttc agaaaatatt acaggcacac gaattgcatt taagatacta tgtaggtccc
     7501 aatgctataa attcagaaat taaagggaca aaactgagaa atgttaaaaa atggatttta
     7561 aaataatact atgattccaa aacaacttgt cgacgggctt ttttcacaat tcggaaccga
     7621 agatatcaat cagctttcaa tccgtcaggt aggaaaactg gttgcacaaa tagagcagca
     7681 gagtggtgtg gaatttatcc acatggaaat gggagttccc ggccttccgg catcacatat
     7741 tggtattgaa gccgaaaaag aagcattaga caaagggctt gcgtctattt atcccaacat
     7801 cgaaggtatc ccgtttgcca aagaggaaat agcccgattc gtacgctcgt ttatgaacat
     7861 cgaaaccgaa ccaacgtatt gtatcccaac tgtaggaggt atgcagggaa gttttgcagc
     7921 tctgcttaca gctgcccgca caaatgctgc gaaaccttac acactattca ttgatccggg
     7981 ttttcccgtt cagaaatcgc aactcaatct gctcggattg cccttcaagg catttgattt
     8041 tcagcatttc aggggcgaaa aactgcgcga taaactggaa gaatttttga aaaacgaaga
     8101 tatttgttcc atttgttact catcgcccaa caatcctacc tggtgttgtt tctctgaaga
     8161 ggaactgaaa actattggtg aactggctga aaaatacgat gtgatagtca tcgaagatct
     8221 ggcatatttc ggtatggact tcagagaaca ttacggaata cccggtgaag caccttttca
     8281 acccactgtt gcacgttaca cccataatta catccttatt ttgtctgcat cgaaaatttt
     8341 cagctatgcc ggtcagcgta tcgccatgct cgtattcagc agccaggcgt acaatcgctc
     8401 ttatccgaat ctgagaaaat acttcccggc cgataccatg ggacgcttta taccttacgg
     8461 agtactctat gccactactg caggcacatc acactcagcg caatatgcac tggctaccat
     8521 gcttaaagcc gccaacgacg cgaaaattaa tttcgtggaa ttgcttcacc attacggcga
     8581 aaaagccaga aaaatgaaag aaatttttct gaaacacggt ttttacattg tttacaacga
     8641 tgatgaacgt gaacttgccg acggatttta tttcaccatt gcctatcccg ggcttacgag
     8701 cggcgaactg atgaaaaaat tcctttattt cggcatcagt gccatttcac ttgccattac
     8761 cggcagcgaa aatcccaacg gacttcgcgc ttgcgtatct tttgtcagaa tggatcagat
     8821 ggaagaactg gagcgacggc tggaagaatt tgtacattcc gtttaaagag ttttttggct
     8881 gaaaatgccg cgaagcaatc gtaagtcaga tttaagaaaa gtgaatacat cagaaaaaat
     8941 gaatttctgt acatattcat ttgttattaa tcccgcacca gagtattgca taagtcaata
     9001 cttaattttt tttgaaaaaa accgatcaga taataaaaaa aatattcata caataaatat
     9061 tctgtttttt gtttttttca actgattttt ttgtattttt acatcattat aattgatgat
     9121 taaaaacaac tctcttaaac ttgtttatag taccttaaat ttttaaattt atcatcgaac
     9181 gtaggtttac agtatttttt ttaaaaccga aacgaaacaa tcaaatcata aatttataac
     9241 catacatgga aaacatcaaa acctctgaag taattgactg ggcaagcaat tttatgtcca
     9301 gagcactgaa tgaaattcgg gaagtggaac gcaaagagca aaacaaatac gctgctctgg
     9361 ttcagaatcc gaatgataaa gtattaatga ccaaaatgct ggacgaaact tcgcagattc
     9421 gtgacaacag aaaactggcc aaacgaatcc gcctgctgct caaagaatac ggaacgcccg
     9481 aattttttaa tgccggtgaa cgattgatgc tgacggcttt taattctgtg ggctatttag
     9541 ttccgggcat ttctgttccc attttcaaaa aatacctgcg cgattacacc agtgcaatca
     9601 ttatcaacga acgtcctgat ttgctcaaca gacacctgga cgaacgaaac aaacaaaaaa
     9661 taggacaaaa tgtaaacctg cttggagaag tggtgcttgg cgacggcgaa gcccgtaaac
     9721 gctacgaaca ttatctggaa gctctcaaag accccagaat caactacata tccgtgaaac
     9781 tttcgggtat ttacgcccaa atcaatccac tgaattacga tacctgtaaa aatgaactct
     9841 gcactctgat gggaaacctg tatcagcagt cgatggattt tccttttaag gatgaaaaag
     9901 gggagttgtc gtataaattt gtaaacctgg atatggaaga gtacaaggat gcagaactta
     9961 cgctcgaagt attcaaaacc gtgctttcag agccccgatt caagaacctt actgccggaa
    10021 tagtaataca ggcatacctg cccgatgcac tcaaattaca gggtgaattg ttggaatttg
    10081 gcaagaaacg atttgccgaa ggtggagcac ctattaaaat gcgtttggta aaaggagcca
    10141 atctggccat ggaaactatc gtttcgtcat tgcgcggctg ggaaaatccg atttacgaca
    10201 acaaaattaa ggtagatgca aactatctga aagtgctcga tgtagctctg ttgcccgaaa
    10261 acgccaaagc tgtacatgta ggagtagctt cacacaactt ctttaccatc ggttatgcct
    10321 atttactggc caaaaagctt caggtggacg aattcgttac tttcgaaatg ctcgaaggaa
    10381 tggcaaatca tttacccagg gtaatgcgaa gcctggacaa gcagataatt ctatatacac
    10441 cggtagtaaa cgacgagcat ttcctcaatg cggtttctta cctggttcgc cgtatggacg
    10501 aaaataccgg aaaagacaac tttcttactt attcgttcaa cctgaaagaa ggcagcaagg
    10561 aatgggattt cctgaaaaaa cagtttgaag atgccatggc tatcaaggat acacttgaaa
    10621 cagttcctaa acgaacacaa aacagaaatt tacagcagcc ggctctgata caggatgtat
    10681 tcaaaaatga acccgatacc gattttgacc ttcgtgaaaa caggctttgg gtaaatcaaa
    10741 tcaggaaaaa atggatgaaa tccgaaaagg acgaacccta ccaaatacct gcacaagtgg
    10801 gtgaaaaaga atataccacc aaccataaaa agaaatattt cgaccgcagc caggatagta
    10861 aagtgctcgt atgtgaggta agtacagttt ctgccgaaca aatgaaagaa gtaatatccg
    10921 tagctcagac cgataaatcc ggttggcgta aaaccaccct tgcaaaaagg aacaaactat
    10981 tacagcaatc ggccacaaat ttagccaaca agcgcggtga tctgataggc tgtatggcag
    11041 ctgtaaccgg aaaaaccttc aacgaaggtg atgtggaagt atccgaagcc atagatttct
    11101 gtcggttcta tccaatatcg atgaaaaaat tcgatgaact gaaaaccgta aaaaattctc
    11161 ccaaaggcat cgtacttgtc attccaccct ggaatttccc tacagcaata cccgtaggtg
    11221 gagtagtggc cggactcgca acaggcaata ccgtgatact gaagcccgcc actgtggctt
    11281 tccccgttgc ctgggaattt gcacagtgtt tctgggaagc cggtgtaccg aaagatgcac
    11341 tgcaggtcat ttgtcccgac ggctcggaag ccctcaatta tctcacttca aattcctcta
    11401 tcagccatat tattatgacc ggaggcaccg atacggcatt caagttgctt gaaaataatc
    11461 cttcctgtcc gctctcggcc gaaacaggcg gaaaggacgc aatcatcctg actgcaagcg
    11521 gtgaccgcga tcacgccatt ctgaatgtag tgaaatcggc tttcggaaat gcaggacaga
    11581 aatgttcggc ctgctccatg ctggtggtcg aaaaaagtgt gtacgacgat cctgatttca
    11641 aagccaagct caaagatgca accgaaagca tgcataccgg aagtgcgtgg gatggaggca
    11701 atctggtagg tccgatggtg accaatacca acgataaact actacatgcc attgacaatc
    11761 cggaagatgg cgaaagctgg ctcgtagcac ctcaatttct ggataaagac cggtatattc
    11821 tcaaaccttg tgtaaaatgg ggcgtaaaac ccggtagcta tacttttgaa aacgaattgt
    11881 tcggaccact gcttgctgta gtttgcatgg atgatttgaa gcatggtatc gaaattgtca
    11941 actccagtga atatggcctt acatcgggct tacagagcct cgatgaatcg gaacgtaaat
    12001 tatggaaaga tacgctcgaa gccggaaatt tgtacatcaa cagaggaata accggagcta
    12061 ttgtacagcg tcagccattc ggagggatga aacgttctgc atttggcgga ggcatcaaag
    12121 ccggaagcga gaattatgta acctgctttg tgaattttga agaaacaggc gtaactgatt
    12181 cttcgtcatc caattcaacc ggattcaata cgttggccga aatgcttccg gcggatgaac
    12241 gcagcagatt cgtaagcgct gtggaaagct acacccaaaa ctggaaaaac gaattctcag
    12301 tcgaaagagt aactcaggat cttattggtg aacttaatac tttccgttat cttccgataa
    12361 aaaatattgt attgcgtatg ttgcctgagg ataatctgtg tgatgttttg atgtgtgtgg
    12421 cggctgccag aatagctcat acaccgcttg tgatcagtgc tcccggcgat gataaaaggg
    12481 tgaaggaatt cagaagattg tctgctcagg aagtagcgtt gattgttcag tctgatgatg
    12541 aattcatcga caaaatggac gactattacc gtgtacgctg ttgctcggat aagttgccaa
    12601 tgagttacta caagaaggct gcgaagctcg gtaaatacat tgccacggct aaaccgctgg
    12661 tggaaggccg cctggaattg cttcactacc tccgggagca aagcatttca tacgagtatc
    12721 accgttacgg aagtttttct gaagaagacg caaaactcta actgaaacag gataaaagaa
    12781 atgaggaatt taccgggtgt aagttcctca tttttatttg attatattaa ggtgaagcta
    12841 ttctttctca agcacgtaaa gactgcaaac tcccagaaag aaagttttgt atttgagaca
    12901 cctgaatccg tttttcttca gaattgcaat catttcttta ccctgcggaa ataccttcat
    12961 cgaatcagtg aggtatttgt aagccagcgg atcgttggag aatgttttta cggcaaacgg
    13021 aattactgtt ctggtgtaga gcaggtatcc ctgtctgatt acgggagttt taggttcgct
    13081 catttcaagg ataacaaact ttccaagggg cttgagcaca cgatgaattt cgcggatgct
    13141 ttcatccagt ttgtcgaaat tccggatacc gaaagatgtg gtggcagcat cgaatgtttc
    13201 atcagcaaaa cttaaacaac ttacatcttc tttctgaagg gtgattttct cattcagttt
    13261 ttctgcattt attttcattc ttcccacttc catcattcct tccgaaatat caacacccgt
    13321 cacatgttca ggattgagca cgtagcaggc tttgatgcat acgtcggctg tgccgcaggc
    13381 caggtcgagt agttggttat gattgtaggg agttagagtt tcaagcgctt tgtctctcca
    13441 tttgtgtgca agtcccatgc tcatgatgtc attgaacttg tcgtactttc gcgaaatgga
    13501 gttgaacatt ttgtatagtt gtgtcgtctt gtcttcttct gtgtggtaag gtgttacttc
    13561 tttaatattc atgacttatt ttttgtaaac aaataaaggt ataaaaggtt tcaaaatcat
    13621 tctgaaaagg aaaactgatt tttccctgag aaaacttctc tacaaatata aatatttatt
    13681 tatatttaac gaaaaaatat acgaaaatac acgaaaaaat atttttttcg aaaaacgatt
    13741 attatttctg ctttttgggt tttggctgac gcactttttg agtttctacc ggtatttcgt
    13801 ccggttgaat atggttttta ctgtagaaaa atatcagata aagcccccag acaatgaatg
    13861 gaatacttag tatttgtccc atattcagcc acataccact ttcaaatgcc tcctgattta
    13921 atttaataaa ttcgagaagg aaacgggtac cgaaaattcc gagcaggaat acaccaaata
    13981 tcattccaat tttcttgtaa gcttgttttt tccaatacat ccaccaggta actgcaaagg
    14041 ttacaaggca atacaacatt tcataaattt gagtcggatg catgggttct gtttgcccgt
    14101 cgcgtacaaa aataaatccc cagggaagcg atgtggcgct tccgtaaatt tcagaattca
    14161 tcagattgcc caaccggatg caggctcctg ttatggcaac accaatcacc aggcggtcaa
    14221 aaccccagat aaagccttta cgggttacat ttttgttgta ataatacatt gcaataatga
    14281 ttcctattgc tcctccatga cttgcaaggc ctccctgcca gatatacagc aattcccagg
    14341 gttttgccag gtacggattt ccgtatttga aagttatgcc cagtatttcc accggttttg
    14401 ccagcaggtg ccattcataa aacaaacaat gtcccaggcg tgcacctaca atggctccga
    14461 ttaccacata cagaaattgc ttgtcaatcc aggtgtccgg gagtttttcg tgtttataga
    14521 gtttacttac aatcagtaag caaagaaata tacctaacgc ccagagagca ccgtaccagc
    14581 gtacagtcag cggccctatc gaaaataatt ccggatttac attccaggtt atatagttta
    14641 gcagcataat tttatggtta aatccgtcat tctcaatttt aataaagaat ttcagaattg
    14701 attgttaatg cgtaaattgt aacgaaaatt gtttttcaat ttatgtaaag actcaaaata
    14761 attgcttatt actgtgagtg atgtgaccgc agcatgttat gctccatgac aattcttaaa
    14821 tttctttccg cttccgcagg ggcatggatc gtttcgtccc acttttttct ctacgcggat
    14881 gggttcgggc ttttgttgtt cccgggtgtc ctgtttggtc ggatcgttgc ttgcagagct
    14941 acttctggat gcgatttcat ctttctgggt gcggtaccgg ctgtagtccg aacggcgttg
    15001 ctgctctccc tgacgcactt ccttcggatc gcgtgtaggt atctgcccgc gcatcaggat
    15061 agaaagcact ttgcggttga tgctggtcac catttctttg aacaaaccga atgattcgag
    15121 tttgtaaatc aacagcggat ctttctgctc gtagctggcg ttttgtacag actgacgcag
    15181 ttcatccagc tgacgcaaat gctctttcca ttcgtcgtca ataacataaa gcagggtttg
    15241 tttctcaaag tctttgatta cttcacgtcc ttcgttgtta taggctgttt tcagattggt
    15301 ggtaatgttg aatacccgtt taccgtcagt aatcggaatt aaaatacttt caaactgatc
    15361 gcctttattt tcataaatat ccttgattac cggataggcg attgatgcca ttttctcggt
    15421 tttccgtttg aaggtctgac gtgcttcgtc cactactttt tcagtcagat ctttcattcg
    15481 ggtgcttttg aattcgtctt ctgttacggg agaatccatg gcaaatacct tcatcagatc
    15541 caggttgaat ccatcataat cttcgtcact tttagccatt tctatcattg cttcagctcc
    15601 gtcgtatatc atatttgaaa tgtccactcc aatacgttcg ccaatgagtg catggcggcg
    15661 ttttccgtaa atcacttcac gctgtgcatt catcacatcg tcgtattcga gcagtctttt
    15721 acgtattccg aagttgtttt cttcgacttt tttctgcgcc cgttcgatgg attttgaaat
    15781 catgctgtgt tctatcactt ctccttcctt gaatcccatt ctgtccatca taccggagat
    15841 tctttcggag ccgaacaatc gcatcaaatc atcttccagc gatacgaaga aaacggatga
    15901 acccggatcg ccctgacgtc cggcacgccc acgcaactgt cggtccacac gacggctttc
    15961 atgccgttcg gtgcccacaa tggccagccc gcctgcagtt tttacctcgg gagttagttt
    16021 gatgtcggta ccacgtccgg ccatattggt agcgatggtt accgtccctt tccgtccggc
    16081 ttcggctacg atgtcagctt cacgctggtg taacttggca ttcagcacgt tgtgttttat
    16141 tccccgcccg gtgagcatac gactcagcaa ttctgaaatt tccaccgagg tagtaccaac
    16201 caatacgggt cgtccggcgt taaccagatt taccacttct tcgatgacag cctgatattt
    16261 ttcacgtttg gttctgtaaa cccggtcttc catatcgttt cgggcaatgg gccggttggt
    16321 cgggattacc acaacatcaa gtttgtaaat gttccagagt tcacctgctt cggtttcggc
    16381 cgtaccggtc atacctgcca gtttgtgata catacggaaa tagttctgaa gcgtgatggt
    16441 ggcaaatgtt tgtgtagccg cttccacttt tacgccttcc ttggcttcga tggcctggtg
    16501 taatccgtcg gaataacggc gtccctccat gatacgtccg gtttgttcat ccacaatttt
    16561 tacctgattt tcgatgacta catactctac atcccgttcg aaaagtgtgt aggctttcag
    16621 cagttgattg atggtatgta cccgttcgga ttttacggaa taattctgga gtatttcgtc
    16681 tttttttgcc tgtttttctt ctttgttgag ggtggtgttt ttttccagtt cggctatttc
    16741 gtcacccacg ttgggcagaa caaagaggag tggatcgtca gaattgccgg taataaggtc
    16801 aattcccttg tcggtcattt cgatggaatt gtttttttcg tcgatgacaa agtatagcgg
    16861 atcagtagct tcgggcatcc ggcggttgtt ttgttccatg tagatttctt cggtcttcag
    16921 catggcggtt tttacaccct gttcactcat gtatttaatc agaggtttgt ttttgggcat
    16981 ccctttgtag gaccggaaaa gagccagcgt accttcatca cgtacttttt cgtcgtcgga
    17041 tttcatcagg ttacgggcgt cggccaggta tttggttacc agctggcgtt gtgcgctaac
    17101 cagtttttcc actttaggcc tcagttcatc aaaaagctga tcttcacctt tcggtatcgg
    17161 gcctgaaata atgagtggag tacgggcatc atctatcaaa acggagtcaa cctcatcgac
    17221 aatggcgtag ttatgcatgc gctgtaccag gtcgagcggg cttgtagcca tgttgtcgcg
    17281 caggtaatca aacccaaatt cgttgttcgt accgaaggtt atgtcggcca ggtacgcctg
    17341 acgtcgttct tcggagttgg ggcggtgctt gtcaatgcaa tcaaccgaaa gtccgtggaa
    17401 catataaagc ggtcccatcc actccgagtc acgcttggag aggtaatcat ttacggttac
    17461 tacgtgtact cctctgtgtg tgagtgcgtt aagaaatacc ggcagggtgg ctacgagtgt
    17521 tttaccttca ccggttgcca tttcggcaat ttttccgctg tggagcacta cgccaccgaa
    17581 tagttgcaca tcgtagtgca ccatatccca tttgatgagg tttccgccgg ccatccattc
    17641 gtttttgaaa atggctttat cgccttctat ccttacaaag tcgtattttg cagccagatt
    17701 tcggtcgaaa tcggtggcac ttaccactac ttcggcattt tcattcagcc ggcgtgcggt
    17761 atctttcatt attgcaaatg cttcggggag tatttcgatt aagactttct cgattttttc
    17821 ggtgatatct ttttctattt tatctacttc gttgtaaatt ttttcgcgca ggtgaagttc
    17881 tgtatcttca atacttgctt ttaattcagc tatccgggca ttgtcgggag ctattgaatc
    17941 ctgaattttg tttttcagtg tttcggttcg cgctcttaat tcatcgttgc tgagtgattt
    18001 tatattttcg taagccgctt taattttttc cacttccggc gatatttctc tcatatcgcg
    18061 ctgggctttg ttacccataa tttttttaag aaaatcgttt aatcccatgt tttatgtggt
    18121 tgttttattt atttcaagaa aattattgtt gatgaaataa tgattagtat tgtttctatt
    18181 atttgatttt cagcagttta ttcttttttt tgattaattt ttacagcatg ctgaaaattc
    18241 atgcaaagat agtgtttttt ggtagtatta tcgggtaaaa actgtattaa aatatgttgt
    18301 ctgattgagg aagggcgtgt agcaggtaac attgttgaga aaaaaccggt tgtaaaacca
    18361 ttcattttct ataaatcttt tagcagaatt ttctcgtgaa tcaggtctga tttttcctgt
    18421 attcctatca ttttttgcca gaaggcatcc atttcttcgt tttttgattc cagaaagagt
    18481 tctgaacctt tcatgtcgag tttcatttcc atcgttttga gggtgagttg gttgatgtcg
    18541 atggcgggca gatatgcttt tacacctttt ctggactctg tagaggcaat gatctgggat
    18601 tcggtaaggt cggtaaggat tgtattcagc agtctgatag gaagtttgta ctgacttact
    18661 atttgctcct gggtcaaagg tggttcgttt gcctgaaagc gtttcacaat gacgtaagtg
    18721 acaaacatcg tgaggcttct tttgtatctg ttgcttatgt tgtcggtgtc cacttcgtaa
    18781 tcaaaatgac gcagattttg cgaaacgtat gaaatttcgg cgccaataag aaaaataagg
    18841 caggaaatat tcagccagat aagtaatagc ggtatggcgg caaagccacc gtaaatggca
    18901 ttgtagcgtg tcaggtttat ctgtccgctc acgtacaaac cctgaaatgc ctgaaataca
    18961 atacctgtaa tcattccggc aatgagtgca ttcagaaaac gtacttttgt gttcggaaca
    19021 atgagataaa tgagtgtgaa taacagtccg ctggtgagat atggaatggt ggaaatgatg
    19081 aacttcatta tcggagtaaa aatctgaaaa aggaatgatt ccttcagaat ggtgtttaca
    19141 taaatagaaa gcccgctgct gatggccatc aaaatgggaa atataagtaa acccgaaaaa
    19201 taaagagtga attgtttaag tacagaccgt gatttctgta cctgccaaat gctgttgagt
    19261 gcagtttcga tttgtgcaaa aagattgtaa accgaatata caagaattcc cagtccgatt
    19321 ccgataaata atccgccgct catcgaatcc aggtattttc ttacaaattc catgatggta
    19381 ggtatgaggt tggcctgtgc agcaaatgta tcctgcaaag ccgtttcgat gatggcttct
    19441 attccgaaac ctttggcgat ggccgaaata agtgcaatga gcggtacaat ggcaaatgaa
    19501 atggagtagg tgagtgcgga agcccggata ccgagcttgt ttttggtgaa acctcttaca
    19561 gaaaggtaaa tggttttgat aatattgtag gtaatccggc gtgttttggt cagctcgttg
    19621 ccggtgattc tccaaatatc gaagcttacg aatttaaacg catactttat ccagtctgac
    19681 agttttttca ttttgtatga tgctcaaagt ttgagataca aagttgaatt caggtaatca
    19741 cttataccgg ataagtgttc atctgtatgg tgaataaaaa aaacagcaga tctgtaagcc
    19801 gggttctgta ttgtacacgt ggcacaattg tttgtcattt atctggttct gccatcactg
    19861 accgaatcta gcaatctacc ccccggcatc ggacgagcca tcctactgcg ccggtataca
    19921 tgatcttgca acccataagg tgtacggcaa tctgtgttgc cacaggctcc ggtgggctct
    19981 taccccgcct tttcaccctt accccgacac ctcatccgcc ctgcgggcac cctctcccga
    20041 aggagagggg aaatggaaag gtgatttcgg agcggtaatt ttctgttaca ctactttgcc
    20101 atcactgaca acttcccgtt aagaagtatg gtgctctgcg ttgcccggac tttcctcaat
    20161 cccgcatgag cgggacagcg acaaaccgaa ctgctgttca tttgcaaaga tactatcttt
    20221 ttgattaata cgcaatatca gtacaatatg tgaggtttta tgagtggaaa atgcttacaa
    20281 tctattatcc ttatgaagaa ataattcatt aataaaaact aaaattgatg ataattcata
    20341 agaaaattag ttacctttgg tggttatttt gtcacatcaa aaaaataagt tataactaaa
    20401 aacaaaacat cttatgaaaa cagaagaaga cgatttattg atttatgacg acgacgatgc
    20461 cgtaaaattc atcgtaaatc tggtaaccgg cgaaatgaaa gaccggatta ccgaagataa
    20521 cgtaaattat gtattggatg tactgtatga tttctacgat gaaaaaggat atatgggcga
    20581 tgatattgcc gaagaagcaa gtatagatga agaggaaatg ctgaaatatg ttgtaaaagc
    20641 tgcaaaaaaa gatggatttg accttaccga ggatgaaatt cagtttgttc tggatggtga
    20701 atatgagtac gggaaatcgc tggggattta tactgacgaa gagtaaaaaa taaaaatttg
    20761 aattgtattg tgaaacacct tgacatacgt atgtcggggt gttttttcct ttattgaaag
    20821 atgacttatt atattgtata cggatttgta aaactcttgt cattgcttcc tttcgcggtg
    20881 ctgtatgcat tgtcggatgt gctttacccg gttatttatt atatcatcgg atacagaaaa
    20941 caagtggtta gagacaatct cagctattca ttccccgaaa aaacagaaca ggaaagaaaa
    21001 gcaatcgaaa agaaattcta tcgtcatttc tgcgacctga tttttgaaaa catcaaattt
    21061 gcaggcatca gtgtaagtga aattaacaaa cgaatgctgt acaacaactt cgaacctgcc
    21121 ctgaagcacc ttgatgaagg gcgaagcgtg atgttatact cttcacacta cggcaactgg
    21181 gagtggaatt catcatttat attacacttg ccaaaagata tgcccattta tccgatttac
    21241 aaaaaacaaa ccagcgaggt gtctgacaga ataattcaca aaataagaaa tcatttcggc
    21301 accgagacta tcgagatgaa aaacctgctt cgtgtgatgg ctgccatgcg cagtgagggc
    21361 aggttcggga tgttcggaat gatttcagac cagtcgccca cgcgcgaaag tttgcattat
    21421 ttcactcaat tcctgaatca gcatacagct gtaattaccg gtacggaaca aattgcacgc
    21481 aaatttgact atccggtgta ttatgcccgc ttgaaaaaac tgaaaagagg ttattacact
    21541 tccgaactga tacccgtttc acttcatccg aaagaaacac cggaatttga aatcagcgaa
    21601 aaatacatgc gattactcga ggaagacatc ctgcgtgacc cggctatctg gttgtggact
    21661 cacaaaagat ggaaatacac gagggaaaaa acggttgaat agttatataa aattaattgt
    21721 tgagccattt taggagcttg attcaattat atcaaatatt aaaaaatctt atttatcata
    21781 t
//
LOCUS       DAORKP010000017        21731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_100_length_21731_cov_8.591945, whole genome shotgun sequence.
ACCESSION   DAORKP010000017 DAORKP010000000
VERSION     DAORKP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21731
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_21731_cov_8.591945"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLN75_01950"
     CDS             complement(<1..292)
                     /locus_tag="PLN75_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRG02909.1"
                     /translation="MIGDEYINVKGHNVDADLGLGCGIPTQFANIKEGDSVLDLGSGA
                     GNDCFVARAIVGETGKVTGIDFTDAMTEKARANNAKLGFTNVEFIQGDIEEMP"
     gene            complement(415..840)
                     /locus_tag="PLN75_01955"
     CDS             complement(415..840)
                     /locus_tag="PLN75_01955"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRG02910.1"
                     /translation="MKVLILCTGNSCRSQMSHGFLQSFNPNITVCSAGTQASGKLNEK
                     AVAVMKEAGVDISHHTSDSVDKYLKDEWDYVITVCGGANEECPAFFGKVKHRLHIGFD
                     DPSHAVGTAEFIDSEYIRVRDEIKEAFWRFYMQEIKPNL"
     gene            complement(882..1220)
                     /locus_tag="PLN75_01960"
     CDS             complement(882..1220)
                     /locus_tag="PLN75_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRG02911.1"
                     /translation="MRKPEVFDTELQELAAFAKVISHPARLAILKYLAETKTCISGDI
                     SDKLPLSRTTVSQHLKELRDMGLIHGEIDGLKINYCLCSSTISEKLDKFDRFFSEIKS
                     AGISCNTNCD"
     gene            complement(1302..2345)
                     /locus_tag="PLN75_01965"
     CDS             complement(1302..2345)
                     /locus_tag="PLN75_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRG02912.1"
                     /translation="MEKTKRTKLILSISAGLPAIILLYYFLQPLVDQFTYGLLGLNPD
                     THLGVSINFFFYDTIKILILLFVISTLMGVINAYFPIDRLRNYLTTRKLYGLQYFFAS
                     FFGAITPFCSCSSIPLFIGFVKGGIPLGVTFAFLITSPLVNEVAVAMFLGLFGWKTTL
                     IYAGSGIFMGIVGGYTLGRLNLDRYLSPWVKELQANSEKESENWEVEKTPFIDRLPAI
                     IKEGWDIVSKVLIYVIIGIAIGAAMHGYVPENFFAKYLSADRWYAMPLAVLAGVPMYA
                     NAAGIIPVIQVFVAKGVPLGTAIAFMMAVVGLSLPEATLLKKVMTWKLIGIFFGTIST
                     FIIILGYLFNRIL"
     gene            complement(2432..3130)
                     /locus_tag="PLN75_01970"
     CDS             complement(2432..3130)
                     /locus_tag="PLN75_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRG02913.1"
                     /translation="MEILQSILDNTQYSFITAIILGLMTAISPCPLATNISAIGFISR
                     DLKDSKRVFISGLVYTLGRAISYTGLALIIFFGASKMNVSMLFQGWGEKLLGPLMILI
                     GLFMLDVIKIKLPGLSGLTDKIGEKSKSSYWSTLLLGMVFALAFCPYSGVLYFVMLIP
                     ITVASASGLYLPVLFAIATGLPVIIFAWLLAYAVGNVGKLYNQIKTFELWFRRAVSVI
                     FIGVGIYYVVIFFL"
     gene            complement(3160..3582)
                     /locus_tag="PLN75_01975"
     CDS             complement(3160..3582)
                     /locus_tag="PLN75_01975"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRG02914.1"
                     /translation="MKRFAIFSVMLSIVLGSMSCDAQTKKTPKATKEVSSKVEVYYFH
                     YTRRCITCNAVEEVTRKSLAEMYPEQQKSGKITFTSVNLDDENSKTIAQKCQAEGQAL
                     LVVSNAQRVDLTDKGFMYARSKPEKLKEEIKNAIDPLL"
     gene            complement(3605..3844)
                     /locus_tag="PLN75_01980"
     CDS             complement(3605..3844)
                     /locus_tag="PLN75_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRG02915.1"
                     /translation="MDIKILGTGCPKCKSLEKLTREVVEQNGLSAEISKVEDIMEIMK
                     FNVMSTPALVVNGKVEIKGRIPSAGEIKAILSKYE"
     gene            complement(3876..4208)
                     /locus_tag="PLN75_01985"
     CDS             complement(3876..4208)
                     /locus_tag="PLN75_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRG02916.1"
                     /translation="MNTTRNKDYTIDNEQLARFAKAMGHPARITILEYLSSLDCCYFG
                     EIHNELPIAKATVSQHLKELKDAGLIQGEIETPKVKYCINKENWEKAQAYFETFFALN
                     KEKIRCCN"
     gene            complement(4393..5046)
                     /locus_tag="PLN75_01990"
     CDS             complement(4393..5046)
                     /locus_tag="PLN75_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRG02917.1"
                     /translation="MKRTVFLILSFCLTISVFAQATKSIRLGEPSASRKTDVMKAFQN
                     RQSTNEYQSKDLSLQDLSDLLWAANGINRPQNGKKTAPSAMNSQDIDVYVCRADGVYL
                     YDAAKKELRQVAKKDLRPLMNGNRPNDAPVLLLLVADLSRYKSYVPGNDEANKRLRDM
                     SALDAGIVSQNISILCAAAALGTRPRAYMNHDELRKELNLTNSQIIWLNHPVGYTKK"
     gene            5176..6060
                     /locus_tag="PLN75_01995"
     CDS             5176..6060
                     /locus_tag="PLN75_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRG02918.1"
                     /translation="MKFGKVEIPYLVDFTLPADHPATANVLPGAKSDEFNVYVGCARW
                     SKSDLKGFYPRGTKDELTYYSTQFNSIELNATYYTQPSDGQVIRWRDKTPADFRFFPK
                     ITNSISHYRRLLNVNQLTDAFCDAVIHFEEKLGTVFLQLNNQFSPREFTKLAAFVEYF
                     PKSIPLAVEVRNEQWFSDPQTWDEFCSLLQSNNVTNLITDTAGRRDLLHQRLTTSSAF
                     IRFTGSNIYEIDYLRMDEWLERIGKWQKKGLKNLFFFIHQNMEASSPFLASYFIEKVN
                     SMFGKSMHVPVKLEKTHR"
     gene            6243..7967
                     /gene="recJ"
                     /locus_tag="PLN75_02000"
     CDS             6243..7967
                     /gene="recJ"
                     /locus_tag="PLN75_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRG02919.1"
                     /translation="MINTWIYSELTDEQNEIKNKLANELSISPVLAKLLVQRGIVTFD
                     DARSFFRPDLNDLHDPFLMKDMDKAVERLTKAMQRNEKILIYGDYDVDGTTSVSLVYR
                     FLVKFYSNLDFYIPDRYNEGYGISIKGIDFAAQNNFKLIIALDCGIKAVEKIKYAKTL
                     GVDFIICDHHNPDDELPPAVAVLDSKRADCNYPYKHLSGCGVGFKLMQAFAMENNIRF
                     EELIPLLDLVALSIASDIVPITGENRILAFYGLKQINSNPSTGVKAIIDVCGLKDREI
                     SISDIVFKIGPRINASGRMKLATEAVELMVSRDYNFAYERSGTINEYNNDRKDLDKNI
                     TDDAIALIRQDDTYSERKSIVVFKPDWHKGVIGIVASRLAEEYYKPSVVLTESNGFAS
                     GSARSVLGFDLYKAVESCKDLLENFGGHMYAAGLTMKIEHVDEFSRRFEKYVAEHIEE
                     DQTYPQIDIDAIIEFKDITPKFFRVLKQFGPFGPGNMKPVFESKNVYDFGSSRLVGKD
                     QEHLKLELTDSSSENVMSGIAFRMYEFNDHLKALNPLDICYTLEENNFNGNTSIQLMI
                     RDIKKVEK"
     gene            complement(8019..8597)
                     /locus_tag="PLN75_02005"
     CDS             complement(8019..8597)
                     /locus_tag="PLN75_02005"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRG02920.1"
                     /translation="MIYSEQNHPNQVRRGSIEVICGSMFSGKTEELIRRLKRANFAKL
                     RVEIFKPKIDTRYSEEDVVSHDRTTIRSTPVESSGSILLMTSEVDVVGIDEAQFFDEG
                     LVDVCKQLANSGIRVIVAGLDMDFRCTPFGPMPALMAIADDVYKVHAICVRCGALAYV
                     SHRLVAGDKQVLLGEMTEYEPICRDCYNHTMK"
     gene            8739..9659
                     /locus_tag="PLN75_02010"
     CDS             8739..9659
                     /locus_tag="PLN75_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02921.1"
                     /translation="MNKEFLVMLLQKDIHELSLLTEGFERMTVFPEPMLRLAKQKAEN
                     IIESLQSLGKNPENELGEIDYQSFSDDEKIENNDVTGTDSGHNSFDIPTGQADELLHS
                     QTDDSEIGAFPSDVPDEDMNEIEAIPETLVEQATERNQEVSVNRNDEIIISLNEEFQE
                     EIEVEEIGENEQILAYEETESASGNNLKETEQTPEETIRENIHNRLIQNSTTLHESLQ
                     KTEISSLGDNLANQKIEDIRQALNIGDRFRFQRELFGNNGETMNKSIAYLNQLAKYEE
                     AVSYLRSKFGWSDDNPHADEFLQIVKRRYL"
     gene            9694..10365
                     /gene="rsmI"
                     /locus_tag="PLN75_02015"
     CDS             9694..10365
                     /gene="rsmI"
                     /locus_tag="PLN75_02015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRG02922.1"
                     /translation="MKLYVVPTPIGNLEDMTFRAVNVLKSVDLILAEDTRTSGFLLKH
                     YEIKTPMQSHHKFNEHKTLQHVIEKLKSGLNIALISDAGTPAISDPGFMLVRACVEQG
                     IEVECLPGATAFVPALVSSGLPNDRFCFEGFLPQKKGRQTRLKSLADESRTMIFYESP
                     FRIVKTLTQLAEVLGPVRKASVSREISKLYEETVRGTLAELIEHFTQNSPKGEFVIVV
                     AGFEE"
     gene            10486..11361
                     /locus_tag="PLN75_02020"
     CDS             10486..11361
                     /locus_tag="PLN75_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02923.1"
                     /translation="MKHNTLLILTISLLLVSCSQPKSGEVERLKAENDSLQSAKLQLE
                     EEVNGYFATLNDIQQNIEKIKDAQNVISVRPLSENTPRDVRNKVSEDMAYINELIKVN
                     KEELEKMRNKMKNSSFQLVNLEKTIASLAKQLNDESAKVARLEIQLQQKDSVITQLGT
                     TVDALGKNVEDLSQKNDEKNALIQQQNEAMNTAWYAIGSIKELKDNKIISSEGIFSAK
                     KVLQSDFNKNYFVRVDTRNTKSIPLYSKAKAKLLTSHPKTSYTLEKENENYVLVITNP
                     AEFWSVSKYLVVEVD"
     gene            11398..12099
                     /locus_tag="PLN75_02025"
     CDS             11398..12099
                     /locus_tag="PLN75_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRG02924.1"
                     /translation="MKNKYKYIFLDLDDTIWDFHANAKESLRDVFHARKLNEHFEDFE
                     NFYRDYSKKNLELWTQYGQGLITKDFLIVERFLHLLQQIGINNPDIALQMNTDFLEIL
                     GTKTGLVPFARELMDFCRMKTLPMTIISNGFVEVQYRKLRSAGIEHYFDHVVLSEAVG
                     TLKPDRKIFEYALNLNKARKEETVMIGDSFDADINGASLAGIDGLFLNHSEKKYELPD
                     NVTEIHSLKDAINII"
     gene            complement(12197..13354)
                     /locus_tag="PLN75_02030"
     CDS             complement(12197..13354)
                     /locus_tag="PLN75_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRG02925.1"
                     /translation="MITYSFLQHYWWFLISLLGGLLSFLLFVQGGQTLIKSLGKTEDE
                     RKLITNVIGKKWHYTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMLILFCFIIQAVS
                     YEYQNKPGNFFGKNAYRGFLFANGLLGTFLLGAAIATFFTGSQFSVNKANIAEFSGQF
                     APVISQWQSPWKGLELLFSDLRVWFFGFTVFFLARTLAVLFLKNRIDNQAIEERLKKA
                     LLMNAVPFLVFFLTFLIWTLLAKGFAVDPATGVVSLEQFKYFNNLIQVPVLLILLLLG
                     VVGVLYGIGFSVLKNTWRKGIWFAGIGTVLTVTTLLLVAGYNNTAYYPSTYDLQSSLT
                     IFNSSSSYYTLKVMSIVSFLIPFVLAYIFYAWRALEKGHATIDEFKEGHSY"
     gene            complement(13393..14940)
                     /locus_tag="PLN75_02035"
     CDS             complement(13393..14940)
                     /locus_tag="PLN75_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRG02926.1"
                     /translation="MELLSTSLVDWSRGQFALTAMYHWLFVPLTLGLGVIQAIMETIY
                     VKTGKEEWKKAAKFWMTIFGINFAIGVATGIILEFQFGTNWSNYSFFVGDIFGAPLAI
                     EGILAFFMEATFISIMFFGWNKVSKGVHLAATWLTITGATLSALWILVANAWMQLPLG
                     MEFNPTTVRNEMVDFWSIISSPVAINKFFHAVASGWVEASVIVLGISSWYLIKKRKEE
                     FAKKSILVAASFGLFAVLFIIFTGHGSAQQVAAHQPMKLAVMEGLYEGREGAPLVAMG
                     VINPKKETYNDDVDPVVMAIEFPKMLSWLSFSDVNAYVPGIRNIVEGGYTKPDGKIAD
                     SFETKKQMGQTAITALKNYKIAEAAKDTAAMAVHKTTLDANYKYFGYGYLDKPEDTIP
                     NVPLVFYSFHIMVILGVLFLLNFALALFLGWKNKLSDYKWILWVFLWTIPLGYLAGQL
                     GWIVAEVGRQPWTIQDILPVQAAVSSLQTSSVLITFVLFAVLFTVLLIAEISIMLKQI
                     RKGPESN"
     gene            complement(14988..15260)
                     /locus_tag="PLN75_02040"
     CDS             complement(14988..15260)
                     /locus_tag="PLN75_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HRG02927.1"
                     /translation="MTKKKISIPRWFRNIYNFYKEGFQNMTLGRTLWAIVLIKLFIMF
                     AILKVFFFPNFLNSKFEKEEDRANYVKSELIDRSTATATITDSISY"
     gene            complement(15546..15881)
                     /gene="cyoD"
                     /locus_tag="PLN75_02045"
     CDS             complement(15546..15881)
                     /gene="cyoD"
                     /locus_tag="PLN75_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HRG02928.1"
                     /translation="MSQSHHENENMGAGHITKKNYIIGFLLATLFTIISFGFVAFKDS
                     MPRSAVVILLSVAALLQMFVHLYYFLHLNKSSEQRWNVIVFAFTALLVFIFVGGTIWV
                     MYTLNLRMM"
     gene            complement(15941..16558)
                     /gene="cyoC"
                     /locus_tag="PLN75_02050"
     CDS             complement(15941..16558)
                     /gene="cyoC"
                     /locus_tag="PLN75_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HRG02929.1"
                     /translation="MNTTEIKNREEAEVIAAEHAHHAELNTFGFWIYLMSDLVIFSVL
                     FATFVVLGKNYAGGPGPKELFHLPYLFIETMFLLLSSVTFGFSMVAMHEHNKKAVITG
                     LIITGLLGLGFIGMELYEFYELIAAGHGPDKSGFLSAFFTLVATHGAHVSFGLLWLIV
                     MIIQLVKQGLSDGVRSRLVRLSLFWHFLDIVWVGVFTFIYLMGMV"
     gene            complement(16555..18567)
                     /gene="cyoB"
                     /locus_tag="PLN75_02055"
     CDS             complement(16555..18567)
                     /gene="cyoB"
                     /locus_tag="PLN75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HRG02930.1"
                     /translation="MFGKLKFTDIPTDPLILSVVAGMILGGVAVVAALTYFKKWGYLW
                     KEWLTTVDHKKIGIMYIIFAMVMLLRGFADGIMMRTQQALAIGSQQGFMDAHHFDQIF
                     SAHGTIMIIFVAMPFLFGLMNLVIPQQIGARDVAFPVLNNISFWFTAAGGAIMMISLM
                     IGEFSNTGWTGLAPLFEKEYNPGVGVDYWLWAFQIAGIGTTLAGINFFVTIIKMRAPG
                     MNLMKMPIFTWASLTSNVLIIISFPVITAALLLLTMDRYLGMHIFTNDLGGNMMLWNN
                     LFWMWGHPEVYIVVLPAFGVFSEVVPTFSRKRLFGYKTMVYAVAVIMFLSFIVWLHHF
                     FTMANHPNVNIFFGITTMMIAIPTGVKVYNWLFTMYRGSITFTTPMYWTLGFLTLFVL
                     GGMTGVLMAIPPADFMVHNSLFLIAHFHNVLIPGALFGYLAGFSYWFPKAFGFRLNET
                     WGKLSFWGWAVGFTLTFMPLYIAGFMGMPRRLASLSNPEWAPYMYVAVVGALIIGFGI
                     FAMLVQLFLSVKDRKKNMDVTGDAWGDGRTLDWSTASPAADYNFAIIPEVKEIDDFAR
                     MKKDGTAYQRPQKYYPIVMPKNKPYGAIIGGLSIAFGFAMVWHIWWLAIGSLIGIIAT
                     VIYFGFDDDNEYVIPAEEVKRIEDERFDAIEAFRAQHTINEKEVKK"
     gene            complement(18586..19590)
                     /gene="cyoA"
                     /locus_tag="PLN75_02060"
     CDS             complement(18586..19590)
                     /gene="cyoA"
                     /locus_tag="PLN75_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HRG02931.1"
                     /translation="MKAAGLLALTFFLSGCEKMIIFNSKGPIGQKESQLIITAFVLML
                     IVVIPVFVMVILFSKKYRASNKNLDYRPDWHDSKKVEWVIWSIPVAIIIVLAALTWKY
                     THELDPYRPIKHELPALQVEVISTDWNWVFIYPEENVASVNELVIEAGRPVRFKLTSA
                     TVMTSFFIPQLGSQIYAMAGMLTQLNLLADEPGTYLGQNIEYSGTGYHKMHFDVHAKT
                     AEEFKVWVQQAKQSPESLSTERYKEISGKQIMDYPVTTFSSVVPNLFQDVMAPYMEWM
                     GHIGVDPGHYHQMNTTDHGSHQMPDSHSGHDMNHDMNHSDHSGHDMHEMKTDTMNMNH
                     "
     gene            20032..20904
                     /locus_tag="PLN75_02065"
     CDS             20032..20904
                     /locus_tag="PLN75_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HRG02932.1"
                     /translation="MENFKKIIRIISELHKFRITFSVTLTTLAGFTLTAGTIDNRIFL
                     PLLGIFFLSSGAAAFNEYQERDMDARMERTRKRPIPSGSIKASTVLVISLPEIVAGTV
                     LVYIGSGFTAALLGFMAFVWYNVIYTPLKKLSPFAVIPGSVIGAIPPMVGWVAGGGSL
                     LDPKLFVLAFFFFMSQVPHFWLLLLRYGDDYDAAGFPVINKILNNTQIKRVTFVWIVA
                     TSVNIVLLVIVGLFQTLFFKIIILLAAIWLVFIFSGLLKKSEEKFNAVRYFMLLNIVL
                     LVIILSMVIDPLLY"
     gene            complement(21119..21613)
                     /locus_tag="PLN75_02070"
     CDS             complement(21119..21613)
                     /locus_tag="PLN75_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRG02933.1"
                     /translation="MKKYVFFILAVFLMNSCQQSLKQAENESMDHSSMSQEQMMAHSN
                     DGVRDGVFIHITESYNDPHKVLMPLKMAVMMANDKDVLIYNDIKSVELYVKGAKDLQY
                     AEFESLQTYVKQLIEKNVGVYVCPTCLKMAGFTEADLMDGVKIAQKDKFFDFTKGRII
                     TLDY"
BASE COUNT         6784 a         4590 c         4208 g         6149 t
ORIGIN      
        1 atggcatttc ctcaatatca ccctgaatga attccacatt cgtaaaccca agttttgcat
       61 tgttggcacg tgctttttca gtcatagcat cggtaaaatc tattccggta actttaccgg
      121 tttcacccac aatggcacgt gcaacaaaac aatcgttacc ggcaccgctg cctaaatcga
      181 gtaccgaatc gccctccttt atattggcaa attgggtagg aataccacaa cccaacccta
      241 aatcggcatc gacgttatga ccttttacat ttatatattc gtctccaatc atgctaaatt
      301 cagactcacc acaacaaccg gaagtaccac aacaagacgt tttttcccga attagacttt
      361 gttgagcaat ttcaccgtat tttttctgaa ttacaaattt tatatccgat ggattcatag
      421 atttggtttt atttcttgca tgtaaaatct ccaaaaagct tctttaatct cgtctctcac
      481 ccgtatatat tcactatcta taaattcagc tgtgcccact gcgtggcttg ggtcatcgaa
      541 accaatgtgc aagcggtgct ttactttgcc gaaaaatgcg ggacattctt cgttagcgcc
      601 accacaaacg gtaattacat aatcccattc atctttcagg tatttatcca cagaatcaga
      661 agtatggtga ctgatatcaa cacccgcttc cttcataacg gcaacagctt tttcattcaa
      721 cttaccgctg gcctgtgttc cggctgagca aactgtaatg tttggattaa aagattgtaa
      781 aaagccgtgt gacatctggc tgcggcagga atttccggtg caaagaatta atactttcat
      841 aaaaattaat ttgaaaattt gagaatgagt aaatttgaaa attaatcgca gtttgtatta
      901 cagcttattc cggcagattt gatttcactg aaaaaccggt cgaatttatc cagtttctca
      961 cttatagtcg aactgcataa acaatagttg attttcaatc catcaatctc gccatgtatc
     1021 aatcccatat cacgcaattc tttcaaatgc tgcgaaacgg tggtgcggct cagcggcagc
     1081 ttgtccgaaa tatcacccga aatgcaggtt ttggtttcgg ccagatactt caaaatagcc
     1141 aaacgcgccg ggtgtgaaat tactttggca aatgctgcca actcttgtag ctctgtatca
     1201 aacacttcgg gttttctcat cactttcttg ttttatgtcg caaacatacg acattaatct
     1261 atgaagtcaa agaaatatct atgatattaa tgttacattt ttcaaagtat ccggttgaac
     1321 aggtatccaa gtataattat gaaggttgaa attgttccaa agaatattcc tatcaatttc
     1381 caggtcatta ctttttttag cagagtagct tcgggaagcg aaagacccac tacggccatc
     1441 ataaatgcga tggcagtacc cagtggcaca cctttagcca caaaaacctg aataaccgga
     1501 attattcctg ctgcattggc atacataggt actccggcaa gtacggccag aggcatggca
     1561 taccacctgt cggcagaaag gtatttagca aagaaatttt cgggaacata cccgtgcatg
     1621 gcagcaccaa tagctattcc tataatcaca taaatcaata ctttacttac aatatcccag
     1681 ccttctttga taatagccgg caagcggtcg ataaagggtg ttttttcaac ttcccagttt
     1741 tccgattctt tttccgaatt ggcttgcaac tctttcaccc aggggctgag atatcgatct
     1801 aaattaagtc ttcctaatgt ataaccccct acaataccca taaaaatacc actaccggca
     1861 taaatcaacg tagttttcca tccgaaaagt cctaaaaaca tcgccactgc cacttcatta
     1921 actaagggtg aagtaatcaa aaacgcgaag gtaactccga gcggtatacc acctttaaca
     1981 aaaccaataa atagcggaat agatgagcag gaacaaaaag gcgtaatagc tccaaagaat
     2041 gaagcaaaga aatattgtaa tccatatagt tttctggttg tcagataatt ccgtaaacgg
     2101 tctatcggaa aataggcatt gataactccc ataagcgtac tgataacgaa aagcagtatt
     2161 aaaatcttaa tcgtatcgta aaagaaaaaa ttaatggata cacccagatg agtatcggga
     2221 tttaaaccca gcaaaccata ggtaaactga tcaaccaggg gttgaagaaa atagtagaga
     2281 agaataattg ctggcaaacc tgccgatata gaaagaataa gttttgttct tttagttttt
     2341 tccatatttg aaattaatca tagttcgtca tattacgaac aatataagta aaaaaaacac
     2401 atttatatcg aaaaattaca gggactgaga attaaagaaa aaatatcaca acataataaa
     2461 tacctacccc gataaaaatc accgaaactg cccgacgaaa ccacagttca aaagttttaa
     2521 tttgattgta aagtttccct acattaccca ctgcataagc cagcaaccag gcaaaaataa
     2581 tcacaggcaa acctgtggca atggcaaaaa gtacaggcaa atatagcccg ctggcacttg
     2641 caactgttat tggtattagc attacaaaat ataagacacc gctatacgga caaaatgcca
     2701 gcgcgaaaac cattcccagc agtaaagtac tccagtaact gcttttactt ttctctccaa
     2761 ttttgtcggt caagccagat aaaccgggta atttgatttt aattacatcg agcataaaca
     2821 gtccgataag gatcataaga ggaccaagca atttttcgcc ccagccctga aacagcatgg
     2881 aaacattcat tttgctcgca ccgaaaaaaa tgataagtgc caggccggta tagcttatcg
     2941 ctcttccaag cgtataaacc aatccactga taaatacccg tttgctgtct ttcaggtcgc
     3001 gacttataaa cccgatagca gaaatgttag tagccaatgg gcagggactg atagcggtca
     3061 tcagtcccag aatgatagcg gtgataaaac tgtattgagt gttatctaaa attgattgca
     3121 atatctccat gcttcaattg aaaatttata aaatgcatat tacaaaagcg gatctatcgc
     3181 attttttatt tcttctttca atttttcggg cttgctacgt gcatacataa aacctttatc
     3241 cgttaggtcc actcgctgag cgttagacac aacaagcaac gcctgacctt ctgcctgaca
     3301 tttttgtgca atagttttac tattctcatc atcgagattg acactggtga acgtgatttt
     3361 tcctgatttt tgttgttccg gatacatttc agcaagtgat ttcctggtca cttcttctac
     3421 agcgttgcaa gtgatgcacc gacgggtata gtggaaataa tacacttcca cttttgagga
     3481 aacttctttg gttgcttttg gcgttttttt ggtttgagcg tcgcatgaca tgcttcccag
     3541 gactatggaa agcatcacac tgaaaatagc aaatcttttc atgttgagtt ttttgttaat
     3601 agaattattc gtattttgac aatattgctt tgatttcgcc tgctgaagga atgcgtcctt
     3661 ttatttctac cttaccattc accaccaaag caggagttga cattacattg aatttcatta
     3721 tttccataat gtcttctact ttcgatattt cggctgaaag tccattttgt tctactacct
     3781 cgcgggttaa tttttcgagt gatttgcact ttgggcagcc tgtgcccaga attttgatgt
     3841 ccattttaaa atttgagttt aaatgtttga aaagtttaat tacaacatcg tattttttct
     3901 ttgtttaacg caaaaaatgt ttcgaaatag gcctgagcct tttcccaatt ttctttgtta
     3961 atgcagtatt taactttggg tgtctctatt tctccctgaa tgaggcctgc atctttcaat
     4021 tcttttaaat gttgtgaaac agttgctttt gcaatgggta gttcattatg aatttcgccg
     4081 aaatagcaac aatccaatga agataaatat tcaagtattg taatacgcgc cggatgcccc
     4141 atcgccttag caaatcgggc aagttgctcg ttatcaattg tataatcttt atttctagtg
     4201 gtattcatgt tgatataatt aattttagtt cgcaaaacta cgaacaatat tctaaactac
     4261 aattatttcc cacaaaaaaa agggccttaa tttttaaata aacataaaaa aagcgcaaga
     4321 aaacatcctg cgcttgttta ataaagcttc tctatttttt ttgaaagaat agtgaaggtt
     4381 ctaatttatc ttttactttt tggtatatcc aaccggatga ttcagccaaa tgatttgtga
     4441 attcgttaga tttagttctt tgcgtagttc gtcgtggttc atataggcgc gcggacgggt
     4501 tccgagtgcg gcagctgcac aaagaatcga aatattctgc gatacaattc cggcatcgag
     4561 cgcgctcata tcccgaaggc gtttgttcgc ttcgtcgtta cccggcacat aacttttgta
     4621 gcgtgataaa tcggctacta aaagcaacaa tactggcgca tcattgggtc ggttgccgtt
     4681 cataagcgga cgtaaatcct tttttgcaac ttgcctgagc tcttttttag ctgcatcgta
     4741 caaataaaca ccatcagccc ggcaaacata cacgtctata tcctgcgaat tcatggcaga
     4801 aggtgctgtt tttttgccat tttgtggccg gttgatgccg ttggcagccc acagcaaatc
     4861 agataaatcc tgaagggata aatctttgct ttggtattcg ttggtcgatt gccggttttg
     4921 aaatgctttc atcacatcgg ttttccggct tgcagaaggc tctccgagtc tgatggattt
     4981 agttgcctga gcaaatacac tgattgtaag gcaaaaagat aaaattaaga aaactgttcg
     5041 tttcatgttt tgtaaaaaat taaaggatta gaaaaaacaa aaatagataa aaatgagcag
     5101 attaaaaaaa atcttttatc aggagaattc atttcgtttg tttcccgtcg gggaatttgt
     5161 cttatctttg cctttatgaa atttggaaaa gtggaaatac cttatttggt ggacttcacc
     5221 ctgcctgctg atcatccggc aaccgcaaac gtgcttcccg gtgcaaaaag tgatgaattt
     5281 aacgtgtacg tagggtgtgc aaggtggagc aaatcggatc tgaaaggatt ttatccccgc
     5341 ggcaccaaag acgaactcac ttattattct acccaattta actccatcga actgaatgct
     5401 acctattaca cgcagccttc ggacggacag gtaatcagat ggagagataa aactccggca
     5461 gatttcaggt ttttcccgaa aataacgaat tccatcagtc attaccgcag actgctgaac
     5521 gtcaatcaat tgactgatgc tttttgtgat gcagtcatcc atttcgaaga aaaactcgga
     5581 acagtatttc ttcaactgaa taatcagttt tcaccccgtg aatttacaaa actggcagcc
     5641 ttcgttgaat attttcccaa atccattccg ttggctgtgg aagttcgcaa tgagcaatgg
     5701 ttttcggacc cgcagacttg ggatgaattt tgctcactac tccaaagcaa caacgttaca
     5761 aatctgataa ccgatactgc aggcagaaga gatttgctgc atcaacggct caccacttct
     5821 tcggctttca tccgttttac aggcagtaat atctacgaaa tagactatct gcgaatggat
     5881 gaatggctgg aacgaatcgg gaaatggcaa aaaaaaggac tgaaaaacct gttctttttc
     5941 attcatcaaa acatggaagc ctcctctcct ttcctggcat cttatttcat cgaaaaagtc
     6001 aattcgatgt ttggaaaatc gatgcatgtt ccggtaaagt tggaaaaaac acacagataa
     6061 aagttttatt ttttttgata ataattaaca acaaaatttc actatcttta tgaacgatta
     6121 taagtgataa ggtgttatag aaaaaagatt gtttgatggg tggattattg gttgaaacga
     6181 ggtgatgatt tgacattgaa atgcaacggc atttcgacct gaagggaata ataataaaca
     6241 aaatgataaa tacatggatt tactcggaac taaccgacga acaaaatgaa atcaaaaaca
     6301 aactagccaa tgaattaagt atcagcccgg ttttagcaaa acttcttgtg cagcggggca
     6361 ttgtaacctt tgatgatgcc cgctcttttt tccgtccgga tttgaatgat ttgcatgatc
     6421 ctttcctgat gaaagacatg gacaaagcgg tagaaagact gacaaaggca atgcaacgga
     6481 atgaaaagat actaatttac ggcgattatg atgtggatgg aacaacctct gtttcattag
     6541 tttacagatt tttggtcaag ttttattcca atcttgattt ctacattccc gatcgttaca
     6601 acgaaggtta tggcatttcc attaaaggaa ttgactttgc tgctcaaaac aatttcaagc
     6661 tgataatagc cctggattgt ggcattaagg ctgtcgaaaa aataaaatat gccaaaacac
     6721 ttggtgtgga ttttattatc tgcgaccacc acaaccccga cgacgaactt ccgcctgccg
     6781 tagctgtact tgattcaaaa cgggcagact gcaattatcc ctacaagcat ctctcgggct
     6841 gtggagtcgg atttaagctg atgcaggcat ttgctatgga aaacaacatc cgatttgaag
     6901 agctgatccc tctgctggat ttggtagcac tcagcattgc ttctgacata gtaccgataa
     6961 cgggcgaaaa ccgcatactc gccttctacg gtctgaaaca gatcaattcc aatccaagta
     7021 cgggtgtcaa agcaatcatt gatgtttgcg gactgaagga cagggagatt tccatcagtg
     7081 acatcgtatt caaaattggt ccgcgaataa acgcctcggg caggatgaaa ctggctactg
     7141 aggccgttga actgatggtt tcgagagatt ataacttcgc ctatgagcga agcggaacaa
     7201 tcaacgaata caacaacgac cggaaagacc tggacaaaaa catcaccgac gacgccattg
     7261 cactcatccg gcaggacgac acctattccg aacggaaatc aatcgtggtt ttcaagcccg
     7321 actggcacaa aggtgtcatc ggaatcgtgg catcacgcct ggcagaggag tattacaaac
     7381 cctcggttgt actgaccgaa tccaacggat ttgcatccgg ctcggcacgc tctgtgcttg
     7441 gtttcgattt gtacaaagct gttgagtcct gtaaagattt gcttgaaaac ttcggcggtc
     7501 acatgtacgc agctggtctg accatgaaga ttgaacatgt ggatgaattt agccgcaggt
     7561 ttgaaaagta cgttgccgaa cacattgaag aagatcagac atatcctcag attgatattg
     7621 atgccatcat cgaattcaag gatattacac ctaaattttt cagggtgttg aaacaattcg
     7681 gacctttcgg accgggcaac atgaaacctg ttttcgaatc taaaaacgtg tacgacttcg
     7741 gcagcagccg attggtgggc aaagatcagg aacacctgaa actggagctg accgacagca
     7801 gttctgaaaa tgtaatgagt ggtattgcct ttcggatgta cgaattcaat gatcatctaa
     7861 aggcactgaa tccgctcgat atctgctaca cgcttgagga aaacaatttc aacggaaaca
     7921 cttccattca gctcatgata agagacatca agaaagtgga aaaataaaca ggcaggcaca
     7981 aaaagggaca agaaaaaact tgtccctttt tgttataatt atttcatcgt atgattgtaa
     8041 caatcacggc aaatcggctc atactcagtc atttcgccca gcagcacctg tttatcgccg
     8101 gccacgagcc ggtgcgatac ataagccaaa gcaccacacc tcacgcagat ggcatgaact
     8161 ttatacacat cgtcggcaat agccatcaga gcaggcatcg gcccaaaagg cgtacacctg
     8221 aaatccatgt cgagccccgc aacaatcaca cgaataccac tgttggccaa ttgtttacac
     8281 acatccacca gaccttcatc aaaaaactgt gcttcatcta ttcccactac atccacttcg
     8341 ctggtcatga gcaggatact accagatgat tccaccggtg tagatcggat ggtagtacgg
     8401 tcgtgcgaca ccacatcttc ttcggaatag cgtgtatcta ttttcggttt gaatatttcc
     8461 acccgcagct ttgcaaaatt tgcccgcttc agcctgcgaa tcagctcctc tgtcttgccc
     8521 gaaaacatgg atccgcaaat tacctcaata cttcctcttc tcacctggtt tggatgattt
     8581 tgctcagaat aaatcattat ttctcgaaat tttggatttt acagtattta attcaatagt
     8641 ttaatttgta tctttacggc agttttaatg ctgatcgtat taatttctac aaaaataaaa
     8701 aaaataattt taccgaactc tttttaattg aaaaagatat gaacaaagaa tttttagtga
     8761 tgctgctgca aaaggatatt cacgaactca gcctgcttac cgagggtttt gagcgaatga
     8821 ctgtttttcc cgagcccatg ctccggctcg caaaacaaaa agctgaaaat atcatcgaaa
     8881 gcctgcaatc tctcggaaaa aatccggaaa atgaattggg tgaaattgat tatcaatcat
     8941 ttagcgatga cgaaaaaatt gaaaataatg acgttaccgg tacagactcc gggcacaata
     9001 gttttgacat acccaccggg caggctgatg agttgctaca ttcacaaacg gatgattcag
     9061 aaatcggagc atttccctca gatgtgccgg acgaagatat gaatgaaatt gaagcaatcc
     9121 ccgaaaccct tgttgaacaa gcaactgaga gaaatcagga ggtaagcgta aatagaaatg
     9181 atgagataat tataagtctg aacgaggaat ttcaggaaga aatagaagtg gaagaaatag
     9241 gtgaaaatga gcaaattctg gcatacgagg aaactgaatc cgcatcggga aacaacctaa
     9301 aagaaaccga acaaacaccg gaagaaacaa taagagaaaa cattcataac cgactcattc
     9361 agaatagcac aacactccac gaaagtcttc agaaaaccga aatcagttca ttgggtgata
     9421 atctggccaa tcaaaaaatt gaggacatcc gccaggcatt gaatatcggc gaccggtttc
     9481 gctttcagcg tgagcttttc ggcaacaacg gcgaaacaat gaataaatcc attgcctatc
     9541 tcaatcagct ggctaaatac gaagaggcag tatcatacct gagatcaaaa ttcgggtgga
     9601 gcgatgacaa tcctcatgca gatgagtttc tgcaaattgt aaaaagaaga tacctctgat
     9661 aacaaaaatt ctgacttctg acctcagcca caaatgaagc tttacgttgt acccacacct
     9721 attggcaacc tggaagacat gactttccgg gctgtgaatg tattgaaaag tgttgatttg
     9781 attctggccg aagacactcg tacgagcggt tttttgctga agcactatga aatcaagact
     9841 ccgatgcaat cgcatcacaa attcaatgag cataaaaccc tgcagcacgt catcgaaaag
     9901 cttaaaagcg gactaaacat cgccttaatt tcagatgcgg gcactcccgc catttcagac
     9961 cccggattca tgctggtgag agcttgcgta gaacaaggta ttgaagtaga gtgtctgccc
    10021 ggtgccacag cttttgttcc ggcgctggta agttcaggtc tgcccaacga ccggttttgt
    10081 ttcgaaggtt ttcttccgca aaaaaaagga cgccaaacca ggctgaaatc gctggcggat
    10141 gaatccagaa cgatgatatt ctatgaatcg cctttcagga ttgtaaaaac actgacacaa
    10201 ctggcagaag tgctgggacc cgtcagaaaa gcatctgttt cgcgcgagat ttcaaaacta
    10261 tacgaagaaa ccgttcgggg aacgcttgct gaattgattg aacatttcac tcaaaactcc
    10321 ccaaaaggcg aatttgtaat tgttgttgca ggttttgaag aataaactta attttgttga
    10381 ccaaataatg catttttgaa aaataaaatg aatttactga ccgtttagtt aatacctttt
    10441 caaagattct ttctgaatat aaataaaaaa atcattcact tcatcatgaa acataatact
    10501 ttactaatcc tcacaatatc attgcttctg gtatcctgca gccagccaaa aagcggcgag
    10561 gtggagcgcc tgaaagctga aaatgattcg ctccaaagtg caaaacttca gctggaagaa
    10621 gaagtgaacg gatactttgc taccctcaat gacatacagc aaaacattga gaaaataaag
    10681 gatgctcaaa acgtcatttc tgtacgcccg ctgagcgaaa acacaccccg cgacgtaaga
    10741 aacaaggtat ctgaggacat ggcatacatc aatgaattga taaaggtcaa caaggaagaa
    10801 cttgaaaaaa tgcgcaacaa gatgaagaac agttcatttc agctggtaaa tcttgaaaaa
    10861 actatcgcct cgctggccaa acaactgaat gatgaatcag ccaaagttgc ccgcctggaa
    10921 attcagttac aacaaaaaga ttcggtgatt acacaactgg gaacaaccgt tgatgcgctg
    10981 ggcaaaaacg ttgaagatct ttcacagaag aacgatgaaa aaaatgcact gattcagcaa
    11041 cagaacgaag ccatgaatac tgcctggtat gccataggct caataaaaga gctgaaagac
    11101 aacaaaataa tctcatccga aggaattttc agcgccaaaa aggtacttca gtctgatttt
    11161 aacaaaaact atttcgtcag agtggacacc cgaaatacaa aatcgatacc gctttattcc
    11221 aaagcaaagg caaaactgct caccagccac ccaaaaacca gctacacgct tgaaaaggag
    11281 aatgaaaact acgtgctggt cataaccaat ccggcagaat tctggagtgt aagcaaatac
    11341 ctggtggtgg aggttgatta aaaaaaaacg acaacaaaga attgccaatt ctcaacggtg
    11401 aaaaacaaat acaaatacat attcctggat ctggacgaca ccatctggga tttccacgcc
    11461 aatgctaaag aatcattgcg cgatgttttt catgcccgaa agctgaatga gcattttgag
    11521 gatttcgaaa atttctaccg cgactattca aaaaaaaacc tggaactctg gactcagtac
    11581 gggcaaggac tcatcacgaa agattttctg attgtagaac gatttctgca tctcctgcag
    11641 cagatcggca tcaacaatcc ggatatcgcc ctgcaaatga ataccgattt tcttgaaatt
    11701 ctgggcacga aaacaggcct tgttcctttt gcacgcgagc tgatggactt ctgccggatg
    11761 aaaactttac ctatgactat catctcaaac ggattcgtgg aagtgcagta ccgcaaactc
    11821 agaagtgccg gaatagagca ttactttgac cacgtagtac tatcagaagc cgtaggcaca
    11881 ctcaaacccg acagaaaaat attcgaatat gcactcaacc tcaacaaagc ccgcaaagaa
    11941 gaaactgtga tgataggcga cagttttgac gccgatatca acggagcatc gctggcagga
    12001 attgatggac tgtttcttaa tcacagcgaa aaaaaatatg aattaccgga taacgtaacc
    12061 gaaattcaca gcctaaaaga tgcaattaat atcatctgac cacaataaac tcccgaaatg
    12121 cactgcactt cgggagtttt ttttaacttc tgccttcttc ccgatagcta tcgggactga
    12181 tttctaattt ttaatttcaa taagaatgac cttctttgaa ctcatcaatg gtagcgtggc
    12241 ctttttccag agctctccag gcatagaaaa tgtaagccag tacaaacggt atcaagaatg
    12301 atacgattga catcacttta agcgtataat agcttgaaga tgaattgaat atcgtaagcg
    12361 aactttgcag gtcataagta gaaggatagt aagcagtatt gttatatccg gcaacaagca
    12421 ataatgttgt aacggtcagc acagtgccga ttccggcaaa ccagatacct tttctccacg
    12481 tatttttcaa aacagagaaa ccaatgccgt aaagtacccc gactacacca agcagtagta
    12541 aaatcaaaag taccggaacc tgtattaagt tattgaaata tttaaattgt tccaatgaaa
    12601 ccactcctgt agccgggtca actgcaaaac ctttggcaag caacgtccag ataaggaatg
    12661 taaggaagaa caccaggaat ggaacggcat tcatcaaaag tgcttttttc aatcgttctt
    12721 caattgcctg gttgtcaata cggtttttca aaaatagtac tgccagcgtt cgggcaagga
    12781 aaaatacggt aaaaccaaag aaccaaacac gtaaatcgga aaacagcaac tctaatccct
    12841 tccagggact ttgccactga ctgataaccg gagcaaactg accggaaaat tcggcgatgt
    12901 tcgctttgtt gacactaaac tgcgaacctg tgaaaaacgt agcaatagcg gcacccagaa
    12961 ggaatgtacc cagcaaacca ttggcaaaca aaaatccgcg gtacgcattt tttccaaaga
    13021 aattacccgg tttattttga tattcgtagg atacagcctg tataataaag caaaacaata
    13081 tcagcatcca aacccagtag gcaccaccga aactggtgga gtagaacaac gggaacgaag
    13141 caaaaaatgc acctccaaaa gtgacgagtg tggtaaaggt gtaatgccat tttttaccaa
    13201 ttacattcgt tatgagtttg cgttcgtctt ccgtttttcc caacgatttg atcaaagttt
    13261 gtccgccctg aacgaaaagc aggaatgaaa gcagcccgcc cagcagtgat attaagaacc
    13321 accagtaatg ttgtagaaat gaatatgtta tcatagttat attaatttat agcgttagta
    13381 ttcgttggtt tgttagttac tctccggtcc tttgcgtatt tgttttaaca tgatgcttat
    13441 ttctgctatc agcaaaactg taaacagcac ggcaaacaga acaaatgtaa taagtacgga
    13501 tgaagtttgc aaactcgaca cggctgcctg aaccggcaaa atatcctgaa tagtccaggg
    13561 ctgacggcct acttcggcca caatccaccc aagctgtccg gctaaatatc ccagtggtat
    13621 tgtccacaaa aacacccata aaatccactt atagtcagac agtttatttt tccagcccaa
    13681 aaataaggca agtgcgaaat tcaacaggaa taacactccc aaaatcacca tgatgtggaa
    13741 cgaataaaac accagaggaa cgttgggaat tgtatcttcc ggtttatcca gatacccgta
    13801 accgaaatat ttatagttag catccagtgt ggttttatgc accgccatgg cagcagtatc
    13861 tttggcagct tcggcaattt tataattttt caatgctgtg atggcagttt gccccatttg
    13921 ctttttcgtt tcaaaagaat cagctatttt accgtcaggc tttgtatatc cgccttcaac
    13981 aatgtttctg attccgggaa cgtaagcatt cacatcactg aatgagagcc atgaaagcat
    14041 tttaggaaat tcaatagcca taactacagg gtctacatca tcattatacg tttctttttt
    14101 cgggttgatt actcccatgg ctaccagcgg cgctccttca cgtccttcgt agagtccttc
    14161 cattacagcg agtttcatgg gttgatgagc agcaacttgc tgggccgacc cgtgacctgt
    14221 gaagatgata aacagcacgg caaataaacc aaaagaagcc gcaaccagaa tacttttctt
    14281 tgcaaattct tctttccgtt ttttgattaa ataccacgaa cttatgccca atactatcac
    14341 agaagcttcc acccagcctg aagccacagc gtgaaagaat ttgttgatgg caaccggtga
    14401 tgagatgata gaccagaaat cgaccatttc attgcgaact gtagtcggat tgaattccat
    14461 cccgagcgga agctgcatcc aggcattggc taccagaatc caaagtgccg aaagcgtagc
    14521 accggtgatg gtaagccacg tggctgccag gtgaacacct ttgctcactt tgttccatcc
    14581 gaaaaacatg atggaaataa aagtggcttc catgaaaaac gccagaatac cttcgatggc
    14641 cagcggtgca ccgaaaatgt cacctacgaa aaaactgtag tttgaccagt tagttccaaa
    14701 ctgaaactcg agaataattc cggtagcaac tccgattgcg aaattaattc caaaaattgt
    14761 catccagaat ttggcggctt tcttccattc ctcttttccg gtctttacat aaattgtttc
    14821 cataatagcc tgaatgactc caagccccag agtcagaggt acaaacagcc agtgatacat
    14881 agctgtgagg gcaaactggc ctcttgacca atcgaccagt gatgtactta ataattccat
    14941 agatttttag tttaattgaa taaaatattt tatatcttaa cgaaatatta ataagaaatc
    15001 gagtcggtaa ttgtggctgt ggcggtagat ctatctataa gttcagattt tacgtaatta
    15061 gccctgtcct cttctttttc aaattttgag ttgagaaaat tggggaaaaa gaagaccttg
    15121 aggatggcaa acatgatgaa aagtttgatg agaactattg cccagagcgt tcgtccgagc
    15181 gtcatatttt gaaaaccttc tttgtaaaaa ttgtaaatat ttctaaacca acggggtatg
    15241 ctgatttttt ttttcgtcat gttttcgttt tactttaacg tgtgattagt agtcgttttt
    15301 gcgatattat tttttcctca ggatacagat aaatcccttt atttgacaca aatatacata
    15361 gttttttttt gaataattgt gttaaattaa aataataaaa aaaatatttt tcttaagttc
    15421 attttgtccc tttttatttc atcaaaaagg tcacctcttt tgaaaaaatt acgggtattt
    15481 ccaaaaagaa aaaatccggt atatcgtgtc gatatgccgg atttcagagt gaaatgactt
    15541 tttaattaca tcattcgcag gttcaatgtg tacattaccc aaatggttcc gcctacaaat
    15601 ataaacacca gtaaagccgt aaatgcaaat acaattacgt tccagcgttg ttctgacgac
    15661 ttgttaaggt gcagaaagta atacaaatga acaaacatct gcagcaaagc agccacagaa
    15721 agcaggatta cgacagcact cctgggcatt gaatccttaa aagccacaaa tccaaatgat
    15781 atgattgtaa agagggttgc cagcaaaaaa ccaataatat agttcttttt agtgatgtga
    15841 cccgcaccca tattttcatt ttcgtgatgt gattgactca ttattattgt ctcctattat
    15901 tttttttatt tctattaaac ttctttttga gaattcacat ttacaccatt cccatgagat
    15961 aaataaaagt gaaaacacct acccacacga tatccaaaaa gtgccagaac agactcaatc
    16021 tcaccaaacg tgaacgtaca ccatcagaaa ggccttgttt taccagctgg ataatcatta
    16081 cgataagcca gaggagtccg aaagatacgt gtgctccgtg tgtggcaacc agggtaaaga
    16141 atgcagaaag aaatccgctt ttatccgggc cgtgtccggc agctatcaat tcataaaatt
    16201 catacaactc cattccgata aagcccaacc ccagcaaacc ggtaataatc agtcctgtga
    16261 ttaccgcttt tttgttgtgc tcatgcatag ccaccatact gaatccgaat gtaacactgc
    16321 tgagtaacag aaacatggtt tcaataaaaa gatacggcaa atggaacaat tcttttggtc
    16381 cgggtccgcc ggcgtagttt ttaccgagaa caacaaacgt ggcaaacaaa accgaaaata
    16441 tcaccaaatc gctcatcaga taaatccaga aaccaaacgt gttcagttct gcatgatgtg
    16501 catgttctgc tgcaataact tcagcttcct ctctattttt tatttcagta gtattcattt
    16561 ttttacctcc ttttcattaa ttgtatgttg agccctgaaa gcttcaatgg catcaaaacg
    16621 ctcatcttca atccgtttaa cctcttcggc agggattaca tattcattat catcatcgaa
    16681 tccgaaatat ataacagttg ctataatccc gattaaactt ccgatagcga gccaccatat
    16741 atgccaaacc attgcaaaac caaaagcgat ggataaacca cctatgatag ctccataggg
    16801 tttgtttttt ggcataacga tggggtaata tttctgggga cgctgatagg cagtaccgtc
    16861 ttttttcatt cttgcaaaat catcaatttc ttttacctca ggaatgattg caaaattata
    16921 atcagcagct ggtgaagctg ttgaccagtc cagggttctt ccatcacccc aggcatcacc
    16981 cgtcacgtcc atatttttct tgcggtcttt tacactcagg aatagctgaa ccagcatggc
    17041 aaaaataccg aaacctataa tcagcgctcc aactactgca acatacatgt atggtgccca
    17101 ttccggattg ctcaggctgg ccagacgtct cggcataccc ataaatcctg caatataaag
    17161 cggcataaat gtcagggtga aacctacagc ccaaccccag aacgagagtt ttccccaggt
    17221 ttcattcagg cggaagccga atgctttcgg aaaccagtag gagaagcctg ccagataacc
    17281 aaacaaagca ccgggaataa gcacattatg gaagtgtgca atcaggaaca aactgttgtg
    17341 aaccataaag tctgccggcg ggatagccat caacacgccg gtcattccac ccagcacaaa
    17401 cagggtgaga aacccaagag tccagtacat aggcgtggta aaggtgatgc ttccacgata
    17461 catggtgaaa agccagttat aaacctttac accggtcggg atggctatca tcatggtagt
    17521 aattccgaaa aatatgttta cattcggatg gttggccatg gtaaagaagt ggtgaagcca
    17581 gacaatgaat gacaaaaaca taatcacagc aacagcataa accattgttt tgtatccaaa
    17641 taatcttttc ctggagaaag tgggaacaac ttccgagaat actccgaatg caggaagtac
    17701 cacaatgtac acttccgggt gtccccacat ccagaacagg ttattccaca acatcatatt
    17761 tcctccaaga tcattggtaa aaatgtgcat ccccaaatac cgatccatgg taagcagcag
    17821 caaggcagct gtgattacag ggaatgagat gataatcaat acattagaag tgagcgatgc
    17881 ccaggtaaaa atgggcattt tcatcagatt catccccggc gcacgcatct ttatgatggt
    17941 aacaaagaaa ttgatgcccg ccagggttgt cccgataccc gctatctgaa atgcccacag
    18001 ccagtaatca actccgactc cgggattata ttctttctcg aacaaaggag ccaaacctgt
    18061 ccagcctgtg ttggagaact ctccaatcat taatgaaatc atcataatag caccaccggc
    18121 agcagtaaac cagaaactga tattattcaa aaccggaaat gcaacgtcgc gcgcaccaat
    18181 ctgctgcgga ataacgaggt tcatcaaacc aaacaaaaac ggcatggcca cgaaaataat
    18241 cataatggtt ccgtgtgcac tgaaaatctg gtcgaaatgg tgagcatcca taaacccttg
    18301 ttgcgacccg attgccaacg cctgttgtgt acgcatcata attccatcgg caaaaccgcg
    18361 aagcagcatg accatggcaa atatgatgta catgataccg attttcttat gatctaccgt
    18421 agtaagccat tctttccata gatagcccca ctttttgaaa taagttaatg ctgccactac
    18481 agctacacca cccaatatca tccctgcgac cacactcaga ataagcggat cggtgggaat
    18541 atctgtaaat tttaattttc caaacatatg tgtatttttc caatattaat gattcatatt
    18601 cattgtatct gtcttcattt catgcatatc atgaccggaa tgatcactgt gattcatatc
    18661 atgattcata tcgtgtcctg aatggctgtc tggcatttga tggctgccgt ggtcggtcgt
    18721 gttcatttgg tggtaatgtc ccggatcaac tccgatgtgt cccatccatt ccatgtaagg
    18781 tgccattacg tcttgaaata aattcggaac aaccgacgag aaggtggtaa ccggatagtc
    18841 catgatctgc tttccgctta tctctttgta tctttctgta ctcaaagatt cgggactttg
    18901 tttcgcctgt tgaacccaga ctttgaattc ttctgccgtt ttggcatgaa catcgaaatg
    18961 cattttatgg taacctgtac cactgtattc gatgttttgt ccaaggtatg ttcccggttc
    19021 atctgccaga agatttaact gtgtaagcat acccgccatg gcgtaaatct gacttccgag
    19081 ctgcggaatg aagaacgacg tcatcactgt agctgaggtt aacttgaaac gaaccggtcg
    19141 tcccgcctct attaccagtt cattgacaga agctacattt tcttcgggat aaataaacac
    19201 ccagttccag tcagtagaaa taacctccac ctgcagagcc ggcaactcat gcttgatggg
    19261 cctgtaaggg tccagttcgt gggtgtattt ccaggtcagt gcagccaaca caataatgat
    19321 agccaccggg attgaccaaa tcacccattc tactttttta gaatcgtgcc agtccgggcg
    19381 gtaatccagg tttttgttcg aagcacgata cttctttgaa aacaaaatta ccatgacaaa
    19441 gacgggaatc acgacaataa gcatcagaac aaaagctgta atgattagct gcgattcctt
    19501 ttggcctatt ggccccttgg agttgaaaat aatcatcttt tcacacccac tcaaaaagaa
    19561 tgtgagagca agaaggccag ctgctttcat tgaattttta aaataatttt ctttcatcat
    19621 acaagggtaa tattttttta aaaaaaatgt tcaaacaaat gcaaatataa ataaatatta
    19681 tattactaca aattttgact aaatttaatc agcaagaaat tttcgatcgc tttttggata
    19741 tttaatgtta ataaatatgt attttattaa atttttgatt gttgacaatg taggaatgat
    19801 gagtctttta ctactgaaag ttggatataa tttcgataca gttcagagcg aaaacattca
    19861 tttcacactg attatcaact attttaattg caaaaatagg acttaaagca tgtgccaaac
    19921 agggaaaaac aaatgaaaaa aatatttttt gtatctttgc ttaatcgttt ttgcatgaca
    19981 acagtatttt tatctgtttt tgattttgca ggctgtatct attaaaaaag tatggaaaat
    20041 tttaaaaaaa taatacgaat aatttcagaa ttacacaagt tcagaattac attttctgtc
    20101 acactaacta ctttggcggg atttacgctg acagccggaa caattgataa ccgtattttc
    20161 ctgcctttgc tgggtatttt ttttctttca tccggcgcag ctgccttcaa cgaatatcag
    20221 gagcgggata tggatgccag aatggaacga acccgaaaac ggcctatccc ttcaggaagt
    20281 atcaaagcaa gtacggtgct ggtaatttct ttacctgaaa ttgttgcggg gacagtgctg
    20341 gtatatattg gcagtggttt tacggctgct ttgctcggtt ttatggcttt tgtgtggtat
    20401 aacgttatat atactccact taagaagttg tcaccattcg ccgttattcc cggcagtgta
    20461 attggtgcca ttccgccgat ggtgggctgg gtggctggtg gtggttcttt gctcgacccc
    20521 aaattattcg tgttagcttt tttctttttt atgtcgcaag tgcctcattt ctggttgctg
    20581 ctgctcaggt atggcgacga ttacgatgct gcgggttttc cggtcatcaa taagattttg
    20641 aataatactc aaataaaacg ggttacattt gtgtggatag tagctacttc ggttaatatc
    20701 gtactgctcg ttattgtagg attgtttcaa actctttttt tcaaaatcat tattttgctt
    20761 gccgctatct ggcttgtatt tattttctcc ggactcttaa aaaaatccga agaaaaattc
    20821 aatgctgtgc gttattttat gctgctaaat attgttctgc ttgtgattat cctctcaatg
    20881 gtcatagatc cgctactgta ttaagagtgg aaagcagcag agtgagaaag cctaagagta
    20941 tcgtgtgtga ggaagggata cagcgcggtc gtatgtttca ggttctggac aggtgaatgc
    21001 taaaaaaagg ggagcattgt ccacaatgat cttgtgagag taattacatt ttttaaaaaa
    21061 agcaaaaaaa gttcacgaag tttcgtgaac tttttttttg ctgaaataat ttttccgttt
    21121 aataatccag tgtgataatt cttccttttg tgaaatcaaa aaatttatct ttctgggcaa
    21181 ttttaactcc atccattaaa tcagcttctg tgaatccggc cattttcagg caggtaggac
    21241 atacataaac gcctacattt ttctcaatca gttgttttac gtacgtttga agtgattcaa
    21301 attctgcata ttgaaggtct ttggcgcctt ttacgtacaa ttctaccgat ttaatgtcgt
    21361 tgtaaatgag cacatccttg tcgtttgcca tcattacagc cattttcaga ggcatcagca
    21421 ctttgtgagg atcgttgtag ctttcggtaa tgtggataaa aacgccatct ctcacaccat
    21481 cgttgctgtg tgccatcatt tgttcctgac tcatggatga gtgatccatt gactcatttt
    21541 cggcttgttt tagactttgc tgacaagaat tcattaaaaa aacagctaag ataaaaaata
    21601 cgtacttttt catgtttatt agtttttgtt aattagtaat cggttgtttt gattcaaaac
    21661 tttaattctt gagtccactc cgaaaagtca taatttataa aaatcttaga tttttattct
    21721 ttgtgttgaa a
//
LOCUS       DAORKP010000018        21552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_102_length_21552_cov_9.078848, whole genome shotgun sequence.
ACCESSION   DAORKP010000018 DAORKP010000000
VERSION     DAORKP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21552
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_21552_cov_9.078848"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1388)
                     /locus_tag="PLN75_02075"
     CDS             complement(165..1388)
                     /locus_tag="PLN75_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG02934.1"
                     /translation="MKKLFLSISFMVGLSAALFAQKANVSQAASLTLQEKPDFKAARE
                     AIKPAFNDEKTKNDPKTYFTAGMIGYTENDSYSKLMMLGKEVDQVKKGKAIMEAYDYF
                     LKAYKLDQMPNAKGKVKPKFDKKIKENIKEFYTAQYNLVGYGAHLFDKKDYKGAFDAF
                     STFLEIPKLPMMNKELSVTDSTYRMIKYFSALAATNMQNHDKAIEMYKDLADDNYETK
                     NVYQLLADEYRTIKDTVNYLATLEQGFKLFNDDPWFLQNIINHYIYSDKIEEASKYLD
                     AAIAQAPTVAEYYYVKGNVDERLGNNDGARKAFEKALELQPKMASAYAGIGRIIFNQA
                     VELLRAADSIRDNKLYNAEVQKANDIFKQSLPYMEKAVELDPKDTDFKQALKMLYYRL
                     GDNANYEKISKELGE"
     gene            complement(1449..3995)
                     /gene="gyrA"
                     /locus_tag="PLN75_02080"
     CDS             complement(1449..3995)
                     /gene="gyrA"
                     /locus_tag="PLN75_02080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRG02935.1"
                     /translation="MTDQEDKIIKVNIDDEMKSSYIDYSMSVIVSRALPDVRDGLKPV
                     HRRVLFGMNELGNNSDKPYKKSARIVGEVMGKFHPHGDSSIYGTLVRLAQPWAMRYTM
                     VDGQGNFGSVDGDGAAAMRYTEARLQKIAEEMLEDINKDTVDFQGNFDNTLKEPKVLP
                     SRIPNLIVNGSSGIAVGMATNMPTHNLSETVDACIAYIENNEIEISDLIKFIKAPDFP
                     TGGIIYGYAGVKEAFETGHGRVVIRARAEIETENGHERLIITEIPYMVNKSELIKAIA
                     NMVSEKRIEGISNITDETNREGMRIVIDIKRDANPGVVLNKLYKYSALQSSFSVNNIA
                     LVNSRPKQLNIKDLIKYFVEHRHEVVTRRTQYELNEAEKRAHLLQGFMIILDNLDEAI
                     RIIRDSQTPDEARTRLMERFELSDVQSRAIVEMRLRQLTGMEQDKLRAEYEDLMKQIE
                     HLKEILEKKELRMEIIKEELLEIKEKYGDKRRTEIVYSSEEFNPEDFYSDDEMVITIS
                     HLGYIKRTALSEFRSQGRGGVGSKGSDFRDEDFIEHIYPATMHNTMLFFTQKGQCYWL
                     KVYEIPEGTKNSKGRAIQNLLNITSDDKVNAYIRVKGLDDKEYINSHYVIFGTKNGII
                     KKTLLEAYSRPRQNGVRAITINENDEVIEVRLTDGKSEIIMANRNGRAIRFNESKVRA
                     MGRTAAGVKGMTLDDDGQDQIIGMICGHTEDDVNILVVSEQGYGKRSSLEDYRVTNRG
                     GKGVKTINITEKTGKLVSIKGVTDDNDLMIINKSGITIRISIANLRVMGRATQGVRII
                     NLEKRNDKIASVCKVSKEEEVVIPEETTDETPLTDTETQSETESEEKNQN"
     gene            complement(4233..4688)
                     /locus_tag="PLN75_02085"
     CDS             complement(4233..4688)
                     /locus_tag="PLN75_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRG02936.1"
                     /translation="MKKLLCKTDSKCCTNLTDWGLLLLRLIVGFFMLTHGWAKLSNFA
                     EMSQQFPPMLGMSSQLGLSLIIFAEFFCSIALILGLFTRLATIPLIIGMGVAAFVAHG
                     ADPFSVKEMSLLYLGIYVTLLLTGSGKIALDTLIYNKFCPHAKKKSDVQ"
     gene            4987..5523
                     /locus_tag="PLN75_02090"
     CDS             4987..5523
                     /locus_tag="PLN75_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02937.1"
                     /translation="MKKLSVIIAFILSFGYSGAQSAGLYELNAGITAQKTQKLYWENG
                     LGVDFTSDFLFGKRIHLKTALITSRLGSAFRSNAINQESFTLGADWRFFSRKPLQIFT
                     GFNAGYFMAHYDDPEFDDLANSSPLVQLDAGLIYRFAFPLSLSASLGYNFITSNGEQG
                     PGTLFPLFYQFKLYYRIK"
     gene            5535..6476
                     /locus_tag="PLN75_02095"
     CDS             5535..6476
                     /locus_tag="PLN75_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02938.1"
                     /translation="MKTDRIYLLFLLFIFVSCNKMPEIDNNTMLDGDQIFDISLVQPE
                     RFLISARYPAPSALQKDIPVIIAAHGYTATTFEWDELRDYADSTGTFYVSQVLLGGHG
                     RSYEEFRKSTWKDWQASIRDEYKKLSRLGYKNIYLAGSSTGCPLIINLIQDGFFENLT
                     KPKKIFLIDPIVISSNKTLTMVNALGPVLGYTSVELTDGEQGKWYVYRPYETLKQLMK
                     LIDLTRKELQSGIRLPQNTKLKTYKSTSDGSADPVSAVLIYKGMKHADGTPVEVEMVD
                     SKLHVFTRLRGRDGVTEKDKALQMKTFKEMEQAMIHP"
     gene            complement(6675..8948)
                     /locus_tag="PLN75_02100"
     CDS             complement(6675..8948)
                     /locus_tag="PLN75_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRG02939.1"
                     /translation="MSKQYLKELAADEELIQKEFEGLLQDYLNSNHRKKVDLITKAFN
                     FAHAAHKGVKRRSGEPYILHPLAVARIVLNEIGLGSTSICSALLHDVVEDTDYTVEDI
                     ENIFGFKIAQIVDGLTKISGGVFGEKASEQAENFRKLLLTMSDDIRVILIKIADRLHN
                     MRTLGSMPPAKQYKIAGETEYIYAPLAHRLGLFRIKTELENLSFKYDHPETYHEIEQK
                     LEGEKKSLNKFYNEFSEPINEKLKSQGYEFELKERIKSVYSIWNKMSTRNIPFEEVYD
                     ILALRIIFESREGMDEKVQCWMIYSAITAIYKPHPERIRDWVSTPKANGYEALHVTVM
                     TPGGRWVEIQIRSRRMHEIAERGLAAHWKYKSGQSDESELDKWLKTIKELLENPEPNA
                     IDFIDTFKLNLFASEIFVFTPKGDIKTIAKNATALDFAFMLHSDIGYQSIAAKVNHKL
                     VPLNTKLNSGDQVEILTSKKQTPQQEWLTYVTTAHARSKLQNYFKKEEKNLMSDGQQK
                     VDNVMTASNIAQSNENMQVILNFYNLSQRSDLYLQVGQGSINAEDIPLILKPKSQSVF
                     SKILKPFVSSSGEKKEKNEKKQKTEEKTETAEKTEKKIDFKKTYKLNELNAGKSYELA
                     NCCRPIPGDDVLGYVTDQGMIQVHKRQCSLAAVIKSNFGNRIVNVEWGAHKAQTYVEL
                     LEVRGIDKVGILIEMLKVISEGYSVNIKKITIESDNGLFVGYFEVLVHDTEDLNNLIS
                     NLLLISEINSVHRVQYN"
     gene            complement(9019..10221)
                     /locus_tag="PLN75_02105"
     CDS             complement(9019..10221)
                     /locus_tag="PLN75_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRG02940.1"
                     /translation="MIQQPKEKSRLFPEECLIREIAGRPRLSIGIPKENPAIETRLAL
                     TPEGVSIITEDGHSVYVQRGAGAPMSYTDLQYSEAGAFLVDDVSEVFGADIVLKITPP
                     EIEELNLMQNRSTVFSMLQLSNLSKEAIELIMLKKMNAFAYELIKDEQKAFPVVNSIA
                     EIEGNTAIAVISDLMSSERGGKGILLGGVAGITPPEIVILGASITGSVAARAAIGLGA
                     MVKVFDHDINKLRKIQHYLGRQVFTSVIHPNVLFKALASADAVIGNLRYIYGSERFMV
                     SEELVKTMKKGSVIVDLSVDQGGCFETSECRTISNPVFEKHGIIHYCVPNISARVGRT
                     SSMALSNIFTSILLKIGNSGSVKQSVADSSGFRHGAYVFNGVMVNRLIGDYFNIPSND
                     IGLLLAGY"
     gene            10373..10948
                     /locus_tag="PLN75_02110"
     CDS             10373..10948
                     /locus_tag="PLN75_02110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRG02941.1"
                     /translation="MANDMMDEVKKAVEVLRQGGVILYPTDTIWGLGCDATNETAVKK
                     VYDIKRRAETKSMLVLIDSPAKLQAYVSEIPDMAWELIELTTKPLTIIYPGARNLASN
                     LINDDNTIGIRVTEEAFSKKLCEMFRKPVVSTSANISGEKSPALFADISKEIISQVDY
                     VVNYRRDEDQPAKASSIIKLGVKNEIQIIRE"
     gene            10956..12359
                     /locus_tag="PLN75_02115"
     CDS             10956..12359
                     /locus_tag="PLN75_02115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRG02942.1"
                     /translation="MNKKLLISKYVFFDIIAALVVWVLFMIFRRVVNDGQLFSSISVF
                     VPNFNYYSNLFLFPILCVAIHYLSGYYVNPIKESKITEFFTTFFSSAIISVIIFFVLL
                     LDDAVVSYQNYYYSLLVLFVLMFTITFVVRAVQSASIRNHFKRKKWTINTLIIGTGEN
                     AGRIADEIQKNSLFNSLVGFIKADSHEKDINQEQIVGSLFDVGRVIDAMNVKEVIVAL
                     DDTNEQRIFNIINKLFQYNIEIQFTPRLYEILTGRVRIQKYGLNPLVSITQPTMTDWE
                     LCVKRSFDIFVSAFSLLLLSPLMLYFFIAIRLDSKGSAIFKQERIGLHGKSFEMLKFR
                     TMQSNAEKGTPKLSSSTDDRVTKVGRMMRKYRFDEIPQFINVIRGDMSIVGPRPERKF
                     YIDQIIQEAPYYCLLYQIRPGLTSWGPIKIGYSDTIDKMIERLNYDIVYMENMNLMTD
                     MKIILSTLEIIFGGKGV"
     gene            12444..14222
                     /locus_tag="PLN75_02120"
     CDS             12444..14222
                     /locus_tag="PLN75_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRG02943.1"
                     /translation="MVDKPWYLKMLAWRERNIKERNFILILSFFVGIFSSLAALILKS
                     AIHLIQNLLTHSFNPDEVNFWYLVFPFMGIVLTYLFVKYVVKDDISHGVTRILYAISQ
                     RKSIIKLHNVWTSIVGSAVTIGFGGSVGAEAPIVFTGSAIGSNMGKFFKLNQKTMLLL
                     IGCGAAGAIGGIFKAPIAGLVFTLEVLMLDMTLTSIVPLLISSVTATSLTYFVTGSSF
                     MFQFTSYEPFSVSRIPQLIILGIICGFVSLYFTRGMNFLEVSFRKIKSPFTKIITGGI
                     TLGVLIFVFPPLYGEGYNTIEALLNGNSKVVLETSYFQHLGGSVWVLVIYLALILFFK
                     IFASTATTSPGGVGGIFAPSLFVGCITGFITATVMNQIGLAAPTSNFSLAGMSGLMSG
                     VMHAPLTGIFLIAELTGSYNLFMTLMIVSTISYMTIIIFEPHSLYAMRLAQKGELITH
                     HKDRAVLTLLRMENVIETDLSVVNPKMTLGELVKVISKSSRNVFPVVNPDNKKFLGIV
                     LLDEVRNIMFRPELYDKFTVQQLMISPPATINIDLPMEKVMQIFEDTGAWNLPVVDVE
                     KRYLGFVSKSKIFNSYRHVLVHFSEE"
     gene            14235..14432
                     /locus_tag="PLN75_02125"
     CDS             14235..14432
                     /locus_tag="PLN75_02125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRG02944.1"
                     /translation="MAKKLLYSDAVAELEKILAELDGNTEINMELISEKVKRAAVLLE
                     VCKKQLHEIDTDLEKILENLD"
     gene            complement(14429..15238)
                     /gene="pgeF"
                     /locus_tag="PLN75_02130"
     CDS             complement(14429..15238)
                     /gene="pgeF"
                     /locus_tag="PLN75_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRG02945.1"
                     /translation="MSSSENIPEVLEYPILSRYTEILHFTSTRSGGVSTGKYASLNLG
                     KYSGDKNENIQENLSRFCSKIGIETQQVYLPFQTHGNSVLIADEEFLAQSAEKQANLL
                     HHTDALITNIPDVCIAVSTADCVPILLYDPENRVIASIHAGWKGTCSRIAANTVQIMQ
                     ATFGVNPKNLIAVIGPSISPAMYEVGAELIDTFMDAGFDVDKIFIRINNKIYLNLWKA
                     NKDILLENGVDESNIEISGYCTYTGQSIFFSARRLGIKSGRMISGIMLKKN"
     gene            complement(15228..16376)
                     /gene="obgE"
                     /locus_tag="PLN75_02135"
     CDS             complement(15228..16376)
                     /gene="obgE"
                     /locus_tag="PLN75_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRG02946.1"
                     /translation="MAIGSNFVDYVKIIGRSGKGGAGSTHFHREKFIPKGGPDGGDGG
                     RGGHVILRGNKNLWTLIHLKYARHVYAGDGGDGSRSRSFGKDGKDQVIEVPIGTVVYD
                     GQTGEFLCDVKDDGQEVILLKGGRGGQGNWHFRTATNQAPRYSQPGEPRIEKTLILQL
                     KILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPNLGIVRHRDNQSFVMADI
                     PGIIEGAAEGKGLGLRFLRHIERNSILLFMIPADSKNIQSEYEVLLNELKKYNPELAD
                     KQRVLAISKCDMLDEEMMEQMKNELPDDVQCVFISSVSGLGIDALKDILWVELNKETN
                     KVVEWIHRPMELTSMEEDEDLDEVEDGDDDDEDDDDLSKYKGIGWDEQ"
     gene            complement(16450..17028)
                     /locus_tag="PLN75_02140"
     CDS             complement(16450..17028)
                     /locus_tag="PLN75_02140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRG02947.1"
                     /translation="MLNLIICGAPGSGKGTQSDLIIKKYNLQHFSTGDILRKEIADET
                     ELGKYAHEFISNGQLVPDGVIIDMIAGKIDKLNKSQINGVIFDGFPRTVAQAEALEEL
                     FEKRGLKTDLLIDLAVDEQELIDRLMLRGKTSGRSDDNLETIKKRLEVYHNQTKPVTE
                     YYEKLGKYASVYGMGTIEDISARIAAAIDEIK"
     gene            complement(17104..17640)
                     /gene="hpt"
                     /locus_tag="PLN75_02145"
     CDS             complement(17104..17640)
                     /gene="hpt"
                     /locus_tag="PLN75_02145"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRG02948.1"
                     /translation="MSTITIKDKQFSLSIKSEVIQEAVSKVGEQINRDMADKNPMFIC
                     VLNGAFMFAGDLMKVVNIPCEITFIKLSSYEGLYTTGSVKEVLGLSESVVGRNIVVVE
                     DIVDTGITMEKILTSLEAKGANSIKVATFLQKPEALQRDIHIDYIAMKIPNEFIVGYG
                     LDYDGYGRNLKDIYTVVD"
     gene            17855..18988
                     /gene="dnaN"
                     /locus_tag="PLN75_02150"
     CDS             17855..18988
                     /gene="dnaN"
                     /locus_tag="PLN75_02150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRG02949.1"
                     /translation="MKFTVSSTELLSHLQAISRVINSKNSLQILDNFLLSLQGNTLTM
                     TASDIETTMITSMEVQDVEGSGTVAVANKLILDTLREFSELPLTFHIDDSTLAMVITS
                     ANGTYNFIGQSGDMYPLLPQLGDNVQHLNVAVPHLLSGISKTFFCMAEDELRPVMNGI
                     YFDISSELTLVATDAHKLVRFKTSYETASVVEGESMSFILPKKPATMLRNILPKESGN
                     VEIKFDDKNAYFELSNYTMVCRQVEGRYPNYNGVIPKNNPFKIIIDRVTLLNTLKRVS
                     VFSNQASNLIKLDFTQNQVHVSAQDIDFSISAEEFIPCQYEGEPIKIGFKSTFLIEML
                     GNVDSAEVVLELADPSRAGILLPVQEDDNEKILMLLMPMLLND"
     gene            19079..19846
                     /locus_tag="PLN75_02155"
     CDS             19079..19846
                     /locus_tag="PLN75_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRG02950.1"
                     /translation="MKLNLKNPIVFFDLETTGTNIVTDRIVEISYLKISPNGREESKT
                     IRINPEMHIPEQVTAIHGITDEDVADCPRFKEVAREIARDFEGCDLAGYNSNRFDIPL
                     LAEEFLRAEVDIDMKKRKFVDVQVVFHKMEQRTLSAAYKFYCDKDLDNAHSAEADTRA
                     TYEVLMAQLDRYPELKNEIAFLSGFTTQNNNADFAGRIIYNEKGEEIINFGKYKGQKV
                     SDVLKNDIGYYGWIMSSDFTLDTKNVLTKIKLRDFQK"
     gene            20013..20711
                     /locus_tag="PLN75_02160"
     CDS             20013..20711
                     /locus_tag="PLN75_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02951.1"
                     /translation="MRRVLKYILFLLLAINIPTFSVAQSTSVIDRTPEQEAVKQTEKM
                     QKELNLTPEQVKNIYEINLKYARERKQSNKRSDAVNRIRSKTEEIGKVLNDRQNYELQ
                     TKRSEVQSVEIDGKRQSTRTDANTRFNSGQNSETNTRTQFRDRPVRTDRSQRQIQTPA
                     SDRYRSTSDKMENPVRRNDSRTEVGRQTRDVPYSVGGSSSSSRSVQQGSTRSSSSESR
                     SSSQPASRESGSRR"
     gene            20722..21435
                     /locus_tag="PLN75_02165"
     CDS             20722..21435
                     /locus_tag="PLN75_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRG02952.1"
                     /translation="MSNPFFKFKQFTVFHDKCAMKVGVDGVTLGAWADVSDVKSILDV
                     GCGSGLITLMLAQRCEARITAIDIDKQSIVQTRENVGNSPWDNRIRVIHTSFQQFASG
                     SSAGFDLIVCNPPFFINSMKSPSESRTKARHSDSLPFLELILNAKKMMTEKGRLCVIL
                     PVPEGKIFNELAVKEKLFCSRLVNVFPNPEKPAKRLLMEYQKEVTDCKTEELIIEKER
                     NLYTPEYSALVKDFYLNKN"
BASE COUNT         5782 a         4473 c         4525 g         6772 t
ORIGIN      
        1 tcatatttcg tattttaaat tgcaaatata atcatttcaa aaacataaag cgctgtagga
       61 attagtactt acagcgcttt tttataaata ttactatttt cggcttttaa aaaataaaat
      121 gcgataacat gttatcgcat tttatttatt ttatcagcaa tggcttattc gcccagttct
      181 ttagagattt tctcgtagtt tgcattgtcg cccaggcgat agtaaagcat tttaagcgct
      241 tgtttgaaat ctgtatcttt tggatccagt tctaccgctt tttccatgta aggtaatgat
      301 tgcttgaaga tatcatttgc cttctgaact tcggcattgt acagtttgtt gtctctgatg
      361 gaatcggcag ctctcaggag ttctacagcc tgattgaaaa taatacgtcc gattccggca
      421 taggcacttg ccatttttgg ctgaagttcc agtgcttttt caaatgcttt gcgggcaccg
      481 tcgttgttgc cgagtctttc gtccacgtta ccttttacat aataatattc ggctacagta
      541 ggagcctgag caatagctgc atcgaggtat ttggatgctt cttcaatctt gtcggagtaa
      601 atgtaatggt tgataatgtt ctgcaggaac cacgggtcgt cgttgaaaag tttgaagccc
      661 tgttcgaggg tggcaagata attcaccgta tcttttattg tgcggtattc gtctgccagc
      721 agctgatata cgtttttggt ttcgtaattg tcgtcagcca ggtctttgta catttcgata
      781 gctttgtcat gattctgcat gttggtagct gccagtgccg agaaatattt aatcatccga
      841 taggtggaat ctgtcactga gagttccttg ttcatcatcg gtagtttggg aatttcaagg
      901 aaagtgctga atgcatcgaa agcgcccttg taatctttct tgtcgaataa atgagctccg
      961 tagcctacca ggttgtattg agcagtgtaa aattctttga tgttttcctt gattttttta
     1021 tcaaatttag gtttaacttt tcctttagcg ttgggcattt ggtccagttt gtaagctttc
     1081 agaaaataat cataagcttc catgattgct tttccttttt ttacctggtc cacttctttg
     1141 cctaacatca taagtttgct gtaagagtcg ttttcggtgt aaccaatcat tccggctgtg
     1201 aaatacgttt tcggatcgtt tttggttttt tcgtcattaa atgcaggttt aatagcctcg
     1261 cgggcagctt tgaagtcagg tttttcctga agggtgagac ttgcagcctg tgatacattc
     1321 gctttctggg caaataatgc agctgataag ccaaccataa aactgatgga taaaaatagt
     1381 tttttcattt ttgtaagtta gtttttatta gttaaatgat tttcgatagt ttaatctatt
     1441 gtttgttttt aattttggtt tttttcttcg ctttctgtct cgctttgagt ttctgtatca
     1501 gtgagcggtg tttcgtcagt tgtttcttcg ggtatcacca cttcttcctc tttgctcact
     1561 ttgcatacgg aggcaatctt gtcgttgcgt ttttccagat tgatgattct tacaccctgt
     1621 gtggctcgtc ccattacgcg gaggtttgca atgctgatcc gaatcgtaat tcccgatttg
     1681 ttgatgatca tcaggtcatt atcgtcggtc actcctttta tcgaaaccag ttttccggtt
     1741 ttttcggtga tgttgattgt tttcactccc ttgccgcccc tgttggtcac ccggtagtct
     1801 tccagtgacg agcgtttgcc gtatccctgt tcggatacga ccagaatatt aacatcatct
     1861 tcggtatgtc cacaaatcat gccaattatt tgatcctggc catcgtcgtc cagggtcatt
     1921 ccttttactc cggcggcggt gcgtcccatg gcgcgcactt ttgattcgtt gaaccggatg
     1981 gctcgtccgt tgcggttggc catgatgatt tccgattttc catcagtgag ccgtacttct
     2041 atcacttcgt cgttctcgtt gatggtgatt gctcttactc cattctgacg cggacgtgag
     2101 taggcttcga gcagtgtttt cttgatgata ccatttttcg tgccaaaaat tacgtagtgt
     2161 gaattgatgt attccttgtc atccagtcct tttacgcgga tgtaggcatt cactttgtcg
     2221 tcggaagtga tgttgagcag gttttgaatg gcgcgtcctt tggaattttt cgttccttcg
     2281 ggtatttcat acactttgag ccaatagcac tgaccttttt gggtaaaaaa gagcatggtg
     2341 ttgtgcatgg tggccggata gatatgttcg atgaagtctt cgtcgcggaa gtcgctgcct
     2401 tttgagccta ctcctccacg gccttgtgaa cggaattcgc tgagcgcagt acgtttgatg
     2461 taacccaggt gagaaattgt gatgaccatt tcgtcgtcgg aatagaaatc ttccggattg
     2521 aattcttcgg atgaatagac tatttctgtc cttcttttgt cgccgtattt ttcttttatt
     2581 tcaagcagct cttctttgat aatttccatt cggagttcct ttttttcaag aatttctttc
     2641 agatgttcga tttgtttcat caaatcttca tactctgccc gcagtttgtc ctgctccatg
     2701 cctgtgagct ggcgaaggcg catttccaca atggcgcgcg actggacatc cgaaagttca
     2761 aagcgttcca tcaggcgtgt acgggcttca tcgggtgttt gtgagtcgcg tatgatccga
     2821 atggcttcat ccaggttgtc cagaataatc ataaaaccct gtagcaggtg agctcgtttt
     2881 tctgcttcat tcagttcgta ttgtgtacgt ctcgttacta cttcatgcct gtgttcgacg
     2941 aaatatttga tcaggtcctt gatattcagc tgtttcggcc ggctgttgac aagtgctata
     3001 ttattcacgc tgaaggatga ttgaagtgcc gagtacttgt aaagtttgtt cagtactact
     3061 cccggatttg catcacgttt gatgtcaatc actatgcgca ttccttcgcg gtttgtttca
     3121 tcggtaatat ttgaaattcc ttcaattctt ttttcggata ccatgttggc aatcgctttg
     3181 atcaattcag atttattgac catataagga atttctgtaa taatgagtcg ttcgtgaccg
     3241 ttttcggttt caatttcggc acgagcgcgg attaccactc ttccgtgtcc ggtttcgaat
     3301 gcttctttta cacctgcata gccgtagata attccaccag tgggaaaatc gggcgcttta
     3361 atgaatttta ttaaatcaga aatttctatt tcgttgtttt cgatgtaggc aatacatgca
     3421 tccactgttt ctgaaaggtt gtgggtgggc atatttgtgg ccataccaac tgctataccg
     3481 gacgatccgt tgacgataag atttggaatt cttgatggca gtacttttgg ttctttaagc
     3541 gtgttgtcga agtttccctg aaaatccaca gtatccttgt tgatgtcttc aagcatttct
     3601 tcggcaattt tttgaagtct tgcttcggtg tatcgcatgg cagcagctcc gtcgccatct
     3661 accgatccga agtttccctg accatccacc attgtgtagc gcatagccca gggttgagcc
     3721 agacgcacta atgtgccgta tatggacgag tcgccgtgtg gatgaaattt acccatcact
     3781 tcacccacta ttctggccga ttttttataa ggtttgtcgg aattgttgcc taattcgttc
     3841 atcccgaaca gtaccctgcg gtgaacgggt tttaacccgt cgcgaacatc cggcagggcg
     3901 cgcgaaacaa ttacagacat ggaataatcg atgtatgacg atttcatctc gtcatcaatg
     3961 ttcactttaa taattttgtc ttcttgatct gtcatttttt ttaattaatt tatacctatg
     4021 aactcactcg attcctgatt ttctgaatag tttgtatatc agtgagttgc taagaatgat
     4081 atgtgtgaaa aacaggtttt ttccggagaa ttaatttgct tcgaaaaatt ggttaaagtt
     4141 acaacttttc gggcaattat acaagtgaat tttaacaaaa aaaggataca attttcatcg
     4201 tatcctcatc gaaagtgatt ttggtatctg ctttattgaa catcggattt cttttttgca
     4261 tgagggcaaa acttgttgta aatcaacgtg tccaatgcta tttttccgga accggtcagc
     4321 agtagtgtta cgtagattcc gaggtagagc agcgacattt cttttaccga aaacggatcg
     4381 gcaccatgag ctacgaaagc agccacaccc ataccgatga tcagcggaat agtagccagc
     4441 cgggtgaata gtcccagaat caaagcaatg gagcagaaaa actcagcaaa aatgatgagt
     4501 gaaagcccca attgtgaact catgccaagc atcggcggaa actgctgact catttcagca
     4561 aaatttgaaa gttttgccca tccatgtgta agcatgaaaa atccaacaat cagtcttagc
     4621 agtaaaagtc cccagtctgt caggtttgta cagcatttgc tgtctgtctt acataataat
     4681 tttttcattt ctttcttttt ttttgatact aattgttcaa acatcagaat gtagttttag
     4741 ttcaatactg tttaaagaaa aattggatat agcttattga tttgatttat atcccgggat
     4801 tttaagctgt tttttaatgt ccgatttttt ttgtaaaata ttttcctgga atgcaattat
     4861 cattcagtca gatagtttcg atgatttgcg aggtaaatgg tcactctttt ttttgctttt
     4921 gcaaatcgtt tttttactca atttttgaat attgtattat atttgccaaa aaacattcct
     4981 ggaaatatga agaaactttc ggttattatt gcttttattc tttccttcgg gtactccggg
     5041 gcgcagtccg ccggtttgta tgagctgaat gcagggataa cggctcaaaa gactcagaaa
     5101 ttatactggg agaatgggct cggagttgat ttcacttccg attttttgtt cggaaagaga
     5161 attcatttga aaacagcttt gattacaagt cgtttgggaa gcgcattcag atcaaatgcc
     5221 atcaatcagg agagttttac actgggcgcc gactggcgtt ttttcagcag gaagccctta
     5281 cagattttta caggttttaa tgccggttat tttatggctc attatgatga tcctgagttt
     5341 gatgatctgg caaacagttc tcctttggtt cagctggatg ccggtctgat ctatcggttt
     5401 gctttcccgt tgtcactttc ggcttctctg ggttataatt tcattacaag caatggagag
     5461 caaggacccg gaacgttgtt cccccttttt tatcaattta aattgtatta ccgaattaaa
     5521 taaaccacgg gattatgaaa actgatcgaa tttacctgct ttttttgctt tttatcttcg
     5581 tatcatgcaa taaaatgcct gaaatcgaca ataataccat gctggatggc gatcagattt
     5641 ttgatatttc gctggttcag cccgaaaggt ttttgatctc ggcacgatat ccggctccat
     5701 cagcactgca aaaagacata ccggtaatca tagctgctca cggatataca gcaaccactt
     5761 ttgaatggga tgagctgcgc gattatgccg acagtaccgg cacattctat gtatcgcagg
     5821 tgctgcttgg cgggcatggc agatcgtatg aggaattcag aaaatcaacc tggaaagact
     5881 ggcaggcatc tatcagggat gaatataaga agttgagccg ccttggttat aaaaatattt
     5941 atcttgcagg ttcatctaca ggctgtcctc tcattatcaa tttgatacaa gatggattct
     6001 ttgaaaatct tacaaagccg aaaaagattt tcctgataga tccgattgta atttcttcca
     6061 acaagacact gacgatggtc aacgcactcg ggcctgtatt gggatataca agtgttgaac
     6121 tcactgatgg cgaacagggg aaatggtatg tttacagacc ttacgaaaca ctgaaacagc
     6181 ttatgaaatt gattgacctg acaagaaagg aacttcaatc cggaatcagg ttaccccaaa
     6241 atacaaaatt gaaaacgtac aaaagcacct cagacggctc cgccgatccg gtgagtgcag
     6301 tgctgattta caaaggcatg aaacatgccg acggaactcc tgtggaggtt gaaatggttg
     6361 actctaaatt gcatgttttt acgcgtctga gaggtcggga tggagtaact gaaaaagata
     6421 aagcactgca aatgaaaact ttcaaagaaa tggaacaagc catgattcat ccataaagct
     6481 cagaagtgaa ttccgtccat cttattattt gattctattc ataaataact gaaatacaac
     6541 aaattgcaat cgattcttga tttccggttt gtatggaagg tttctatgaa aaaagcgact
     6601 gaacgataat tgaagtcgct ttttatatgt ctgtgtggta tgaatatacc tgacatgcag
     6661 cataaaatca acgtttagtt gtattgtacc cggtgtaccg agttgatttc agaaatcagc
     6721 aacagattgg aaatcaaatt attgaggtct tcggtgtcgt gcacgagcac ttcgaaataa
     6781 cccacaaaca atccgttgtc gctttcgatg gtgatttttt taatgtttac actgtaacct
     6841 tcagaaatta ctttcagcat ttctatcagg attcccactt tgtcgattcc tcttacttcc
     6901 aacagttcaa cataagtttg ggctttatgt gcgccccatt ccacattcac aatccggttt
     6961 ccgaaattac tttttatcac agcagccagg ctgcattgcc gcttgtgtac ctgtatcatt
     7021 ccctggtctg taacgtagcc gagcacatcg tcgcccggaa tcggacggca gcagttggct
     7081 aattcgtatg atttgccggc gttcagttcg ttgagtttgt aggttttctt gaagtcaatt
     7141 ttcttttcgg ttttttcagc ggtttctgtc ttttcttccg ttttttgctt tttctcgttt
     7201 ttttctttct tttctcccga tgaagaaacg aatggtttca gaattttcga aaatacgctt
     7261 tgcgatttgg gtttgagaat caacggtatg tcttcggcgt tgatcgagcc ctgccctacc
     7321 tgcaagtaca aatcacttcg ctgcgaaagg ttgtagaaat taagtataac ctgcatgttt
     7381 tcattacttt gagcaatgtt tgatgcggtc atgacgttat ctaccttttg ctgcccatcg
     7441 ctcatcagat ttttttcctc ttttttgaaa taattctgca gtttcgacct ggcatgtgca
     7501 gttgttacgt atgtgagcca ttcctgctga ggcgtttgct tcttggatgt gagaatttcc
     7561 acctgatcgc cactgttgag tttggtgttc agcggcacca gcttgtgatt cactttggct
     7621 gcaatgctct gataaccgat gtcggagtga agcatgaaag cgaaatcaag tgctgttgca
     7681 ttttttgcaa tggttttgat gtcgcctttc ggcgtaaaga caaatatttc ggatgcaaaa
     7741 agatttaatt tgaaggtgtc gataaaatca atggcattcg gctccggatt ttcaagcagt
     7801 tctttgattg ttttcagcca tttatccagt tctgactcgt cgctctggcc gcttttgtat
     7861 ttccagtggg cagccagtcc gcgctcagca atctcatgca tccgtcggct gcggatctga
     7921 atttccaccc accggcctcc aggtgtcatc accgtaacgt ggagtgcttc atatccgttg
     7981 gcctttggtg tgcttaccca gtcgcggata cgttcgggat gtggtttgta aatggccgta
     8041 atggcagaat aaatcatcca gcactgaact ttttcatcca ttccttcgcg tgattcgaat
     8101 ataatacgca atgccaggat atcatatact tcttcgaacg ggatattgcg ggttgacatt
     8161 ttattccaga tggaatagac agatttgatc ctttctttca gctcgaattc atatccctga
     8221 cttttcagtt tctcgttgat aggttctgaa aattcgttgt aaaacttatt cagtgatttc
     8281 ttttcgcctt cgagcttttg ctctatttca tggtatgttt cgggatggtc gtatttgaaa
     8341 ctcaggtttt caagttcggt tttaatccgg aataatccca gtcgatgggc aagcggagcg
     8401 taaatgtatt ctgtttcgcc ggctattttg tactgtttgg cagggggcat tgagcccagc
     8461 gtacgcatat tgtggagccg gtcggctatt tttatcagta tcacgcggat gtcgtcggac
     8521 atggtgagca gcagttttct gaagttttca gcttgttcgg atgccttttc gccaaaaaca
     8581 ccacccgaga tttttgtcag accgtctaca atttgcgcaa ttttgaagcc gaaaatattc
     8641 tcaatatctt caaccgtata atcggtgtct tctaccacat cgtggagcag cgcagaacaa
     8701 atggaggtgg agccgagccc gatttcgttc agtacaatgc gtgccactgc cagcggatga
     8761 agtatatacg gctctcccga acggcgcttg acacctttgt gggcagcatg tgcaaaattg
     8821 aaagctttgg tgatcagatc gacctttttc cggtgatttg aattcagata gtcctgcaat
     8881 aacccttcga attccttctg gattaattcc tcatcagcgg ccagctcctt taagtattgt
     8941 tttgacatag gtggttatct tgagaagtgt ctgaaattct ttaaaaaaaa tgacaaaaaa
     9001 aacaatcagg aaacaatatc agtatccggc cagaagtagt ccgatgtcat tggatggaat
     9061 gttgaaataa tcgccaatca gacggttcac catcaccccg ttgaatacat aagcaccgtg
     9121 tctgaagccc gaactgtcag ctaccgattg ttttacactg ccggaatttc ctattttaag
     9181 cagtatggaa gtgaagatat tgctcagtgc catggaggag gtgcggccta ctcgtgccga
     9241 aatattggga acacagtaat ggataatgcc atgtttttca aaaacaggat ttgatatggt
     9301 gcgacattcc gaagtttcaa aacatcctcc ctgatcgaca ctgaggtcaa cgattacgga
     9361 tccttttttc atcgttttta ccaattcttc ggaaaccata aaccgctccg agccgtagat
     9421 gtatcttaaa tttccgatca cggcatctgc cgaagccagg gctttgaaga gtacattggg
     9481 atgtataaca gacgtaaata cctgacggcc aagataatgc tgtattttgc ggagtttatt
     9541 aatgtcatga tcaaaaacct taaccatagc gccaagcccg atagctgccc gggctgctac
     9601 agatccggta atacttgctc cgagaattac aatttcgggt ggagttattc cggctacccc
     9661 gcccagcaaa atgcctttgc cgccccgctc gctgctcatc agatctgaaa tcacagctat
     9721 ggcagtgtta ccttcaattt cggcaatgga attgaccacc ggaaatgctt tctgttcatc
     9781 ttttatgagc tcgtaggcaa aagcgttcat ctttttaagc atgatcaatt caatagcttc
     9841 tttggagaga ttgctcaact gaagcatcga aaagaccgta gagcggtttt gcatcagatt
     9901 cagttcctct atttctggag gtgtgatttt caacactata tcggcaccga aaacttctga
     9961 tacatcgtca accagaaatg cacctgcttc actgtactgt aagtcggtgt atgacatggg
    10021 cgcaccggct ccccgctgta cgtacacact gtgaccgtct tcggttatta tgctcactcc
    10081 ttcgggggtg agtgccagac gggtttctat ggccggattt tctttcggaa taccgatgga
    10141 gagtcggggt cttcctgcaa tttctcttat caggcattct tcggggaata atctgctttt
    10201 ttctttgggc tgttgaatca taatcgtatc tttgatgact acaaatctaa ttactattcc
    10261 tgaattgagc aaaagtaggt gaactttttt tattctgaaa cacaaaaagc gtctttgctt
    10321 ttattcagaa atgttgaaat aaaaattgtt tttttacgtt tttaaaaata atatggcaaa
    10381 cgatatgatg gatgaagtga aaaaagctgt ggaggttctt cggcagggtg gtgtgatatt
    10441 gtatcctacc gataccatct gggggctggg atgcgatgca accaacgaaa cggctgtaaa
    10501 aaaagtgtac gatatcaagc ggcgggccga aaccaaatcg atgcttgtgc tcattgattc
    10561 gccggccaag ttgcaggcat acgtttcaga aattcctgat atggcctggg aattgataga
    10621 attgacaacc aaaccgctca ccatcattta cccgggagca cgaaatctgg catcaaatct
    10681 gatcaacgat gataatacga taggcatccg tgtgactgaa gaagcttttt cgaaaaaact
    10741 gtgtgaaatg ttccgcaaac cggtggtttc tacttcggcc aacatcagcg gtgagaaatc
    10801 accggctttg tttgcagata tttcaaaaga aattatcagt caggtagatt acgtagtaaa
    10861 ttatcggcgc gatgaagatc aacccgctaa agcaagctcc attatcaaac tgggagtgaa
    10921 aaatgagatt caaataattc gtgaataaca acagcatgaa taaaaaacta ctcatttcaa
    10981 aatatgtttt tttcgatata atagccgccc tggttgtatg ggtccttttt atgattttca
    11041 gaagggttgt caacgacggt caattgtttt cgagcatttc tgtttttgta cctaatttca
    11101 attattattc caatctgttt ctatttccga tcctttgtgt tgcaattcac tatttatcag
    11161 gatattatgt taatcctatc aaagaaagca aaataacaga attctttact acattttttt
    11221 cttcagctat catttctgtc ataattttct ttgttttgct ccttgatgat gccgtagtga
    11281 gctatcagaa ttattactat tctcttttgg tactctttgt gctgatgttt actattacat
    11341 ttgtcgtcag agcagttcaa agtgccagta ttcgtaacca ttttaaaaga aaaaaatgga
    11401 ccatcaacac gctgataatc ggaacaggtg aaaatgccgg gcgcatagct gacgagattc
    11461 agaaaaattc gctttttaat agtttggttg gattcattaa ggctgattct cacgaaaaag
    11521 acattaatca ggagcaaatt gtcggaagtt tgtttgatgt gggcagggtg attgacgcca
    11581 tgaatgtaaa ggaagtaatt gtagctctgg atgataccaa cgaacaacgc atatttaata
    11641 tcatcaacaa actttttcaa tacaacatcg aaattcagtt tactccccgg ttatacgaga
    11701 tcctgaccgg cagggtacgc atacagaaat acgggcttaa tccgctggta agcatcacac
    11761 agcctaccat gaccgattgg gaactctgcg tgaaaagatc gtttgatatt tttgtttcgg
    11821 ctttcagcct tcttttgctt tcgcccctga tgctttattt tttcattgcc atcaggctcg
    11881 actcgaaagg ctccgccatt ttcaaacagg agagaatcgg acttcacggc aagtcgtttg
    11941 aaatgctgaa attcagaacc atgcaaagca atgcagaaaa aggcacgcca aaactcagta
    12001 gctctaccga cgaccgggta accaaagtgg gaagaatgat gcggaaatac agattcgatg
    12061 aaatcccgca gtttatcaat gtaattcgcg gtgatatgag tattgtaggg cccagacccg
    12121 aacgaaaatt ctatattgac cagataattc aggaggctcc ttattactgc ctgctgtatc
    12181 agattcgtcc aggtcttact tcgtggggac cgattaaaat tggctattcg gatacaatcg
    12241 ataaaatgat cgaacggctc aattacgaca ttgtatatat ggaaaatatg aacctgatga
    12301 ccgatatgaa aattatcctg tccacgttgg aaattatttt cggtggcaaa ggtgtctgat
    12361 atttcgtttt tacttttcct gccgaatgct tcccattcaa aataaaaacc attatctttg
    12421 taatcttacg aatatgaaag gtcatggtag ataaaccctg gtatctgaag atgcttgcct
    12481 ggcgtgaaag aaacatcaag gaacggaatt ttattctcat attgagtttc tttgtcggta
    12541 ttttctcttc gctggcagct ttaattctaa aatcagctat tcatcttatt cagaatttgc
    12601 ttactcacag cttcaatcct gatgaggtga atttttggta ccttgtattt ccgtttatgg
    12661 gaattgtact cacctatctt tttgtaaaat atgtagtgaa agatgatatc agccacggtg
    12721 tcacccggat attgtatgcc atctcgcagc gtaaatccat catcaaactt cacaacgtat
    12781 ggacatccat tgtggggagc gcagtcacca tcggttttgg cggttcggtg ggtgccgagg
    12841 ctccaattgt tttcaccgga tcggccatcg gatccaacat gggcaaattc ttcaaactga
    12901 atcagaaaac aatgttactg ctgattggat gtggagctgc cggagctatc ggaggcattt
    12961 tcaaggcacc gatagccgga ctggttttca cactcgaagt gctgatgctt gatatgacgt
    13021 tgacttccat cgtacccttg ttaatatctt ctgttacagc tacttctctt acctattttg
    13081 ttaccggaag cagttttatg tttcagttta cgagttacga accattttcg gtcagccgta
    13141 ttcctcagtt gattattctg ggaatcattt gtggttttgt atcgctttat tttacccgtg
    13201 gaatgaattt tctggaagta tctttccgta aaatcaaatc accctttacc aaaattataa
    13261 caggtggaat tacactgggt gttctgattt ttgtatttcc accgttgtac ggcgagggtt
    13321 acaataccat cgaagcgctg ctcaacggca attcaaaagt tgtactcgaa accagttatt
    13381 tccagcatct gggtggaagt gtatgggtat tggtcattta tcttgccctg attttgtttt
    13441 ttaaaatttt tgcctctacc gctactacga gtcccggagg agtaggtggg atatttgcac
    13501 catcactttt tgttgggtgt attaccggat ttattacagc tacagtcatg aatcagatag
    13561 gcctggctgc tccgacttcc aatttctcgc tggccggcat gtcgggtctg atgtcgggtg
    13621 taatgcatgc acctcttaca ggcatctttc tgatagccga gctcaccgga agttacaacc
    13681 tcttcatgac attgatgatt gtctcgacca tttcatacat gacaatcatt attttcgaac
    13741 ctcacagcct gtatgccatg cggcttgccc agaaaggcga actgattaca catcacaaag
    13801 acagagcggt gcttacgttg ttgagaatgg agaatgtaat cgaaacagat ttatcagttg
    13861 ttaatccgaa aatgactctt ggtgagctgg taaaagtgat ttcaaaatca agccggaatg
    13921 tgttcccggt ggttaaccct gataataaga aatttttggg tattgtgttg ctcgatgaag
    13981 tgcgcaatat catgttccga cccgagcttt acgataaatt caccgttcag cagctcatga
    14041 tttctcctcc agctaccatc aatatcgatt tacccatgga aaaggttatg cagatttttg
    14101 aagataccgg agcgtggaat ctgccggtgg ttgatgtgga aaaaaggtac ctcggatttg
    14161 tgtccaaatc aaaaattttc aattcatacc ggcatgttct ggtacatttt tcggaagaat
    14221 aacagaaaaa tattatggct aaaaaactat tatacagcga cgcagtggcc gaactggaga
    14281 aaatcctggc cgaacttgac ggaaacacag aaatcaatat ggagttgata tcagaaaagg
    14341 tgaaacgtgc agcagtgctt ctggaagtct gcaaaaaaca acttcatgag attgacaccg
    14401 atttggaaaa aatacttgaa aacctggatt aattcttttt aagcattata ccggaaatca
    14461 ttctacccga ttttattcct aatcgcctgg ctgagaagaa tatactctgt ccggtatagg
    14521 tgcagtatcc tgaaatttca atattggatt catcaactcc gttttcgagc agaatgtctt
    14581 tgttggcctt ccatagattc agataaattt tattgttgat gcgaataaag attttatcca
    14641 catcaaatcc ggcatccatg aaagtgtcaa tcagttcagc accgacctca tacattgccg
    14701 gtgaaataga agggccgatt acggcaatca ggttttttgg atttactccg aatgtagctt
    14761 gcataatttg aacggtgttt gccgcaatgc gggagcaggt acccttccat ccggcatgaa
    14821 tggatgcaat cactctgttt tcaggatcat acagaagtat cggaacgcag tcggcggtag
    14881 aaacagcaat gcagacatcc ggaatgttgg tgataagtgc atctgtgtga tgaagtagat
    14941 ttgcctgttt ttcggcactt tgggcgagaa attcttcatc tgctatcaga accgaattac
    15001 cgtgggtctg aaaaggcaaa tagacttgtt gtgtctctat accgatttta ctgcaaaacc
    15061 tggagagatt ttcctgaata ttttcatttt tatcacctga atatttaccc agattcaatg
    15121 aggcatattt tccggtgctg actccaccgg agcgtgtgga tgtaaaatgc aatatttcag
    15181 tataccggct tagaatgggg tattcgagaa cttccgggat gttttcacta ctgctcatcc
    15241 cacccgatgc ctttgtattt gcttaaatcg tcgtcatctt cgtcatcatc atcaccgtct
    15301 tcaacttcat ctaaatcttc atcttcttcc atgcttgtta attccatcgg tcggtgaatc
    15361 cattctacca ctttattggt ttctttattc agttccaccc acagaatatc cttcagagca
    15421 tctattccca atccgcttac cgacgaaatg aaaacgcatt gcacgtcatc gggtagctcg
    15481 tttttcatct gttccatcat ttcctcgtcc agcatatcac atttggaaat agccagtacc
    15541 cgttgcttgt cggctaattc cggattgtat tttttcagtt cgttcaacag cacttcatat
    15601 tccgattgta tgtttttgct gtctgccgga atcatgaaca gcaggatgga attacgctcg
    15661 atgtgcctca ggaatctcag tccgagacct ttgccttcgg cagcgccttc aataataccc
    15721 ggtatgtcgg ccattacgaa agattgattg tcgcgatgac gcacaatgcc cagattcggg
    15781 accagtgtgg tgaagggata gtcggctatt tcgggtttgg cagccgatac taccgacaga
    15841 agcgtagatt taccggcatt agggaatcct accaaaccta catctgccag aattttgagt
    15901 tgcagaatga gtgttttttc tattcggggc tctcccggtt gtgagtatcg tggtgcctga
    15961 ttggtggcgg tgcgaaaatg ccagtttccc tgtccgccac gtccgccttt gagcagtatc
    16021 acttcctgtc cgtcgtcttt cacgtcacag agaaattcac cggtttgtcc gtcgtaaact
    16081 accgtcccga ttggcacttc tattacctga tctttaccgt ctttcccgaa actccggctc
    16141 cggcttccat ctcctccatc accggcatat acgtgccggg cgtatttcag gtgtatgaga
    16201 gtccacaggt ttttatttcc acggagtatc acatgcccgc cccgacctcc gtctccacca
    16261 tccgggccgc ctttcggtat gaatttttca cgatgaaagt gagttgaacc agcaccgcct
    16321 ttgcccgaac ggcctatgat tttcacataa tctacaaaat tggagcctat agccattttt
    16381 tagtttcttg ttgttttgtt ttaaaaaaaa tccaccgata aaaaaatgtc gatggatgtt
    16441 ttgtgctgtt tactttatct catctattgc tgcggcaatt cgagccgaaa tatcttcaat
    16501 agttcccatg ccgtacactg aggcatattt gccgagtttt tcgtagtatt cggtcaccgg
    16561 tttggtttga ttgtgataca cctcaagcct ttttttgatt gtttccaggt tatcatcact
    16621 tctgcccgag gtttttcctc tgagcatcag gcggtctatc agttcttgtt catccactgc
    16681 cagatcaatc agcaggtctg tttttaatcc ccgtttttcg aataattctt ccagggcttc
    16741 ggcctgtgct accgttcgtg gaaatccgtc aaagatcact ccattaattt gacttttgtt
    16801 cagcttatct attttgccgg caatcatgtc aataatcact ccatccggca ctaattgtcc
    16861 gttcgaaata aattcgtgtg cgtatttgcc cagctccgtt tcgtcagcta tttcctttcg
    16921 gagaatatcg ccggtagaaa aatgctgaag attgtatttt ttgatgataa ggtcgctctg
    16981 tgttcctttg ccgctgcccg gtgcgccgca gataattaag ttaagcatgt tgttttttgc
    17041 gttaaaatgg aaaaaaaatt attggtatta ctgttttttt tcaataaaaa atggagtcat
    17101 caattaatct accacggtat atatgtcttt caggttacgt ccgtatccgt cataatcaag
    17161 gccatatcct actataaatt cattgggtat tttcatggct atgtagtcaa tgtgtatatc
    17221 gcgttgcaga gcttccggtt tttgcaggaa ggtggctacc tttatgctgt tggcgccttt
    17281 tgcttccagc gatgtgagga ttttttccat cgtgatgccg gtatctacta tatcttccac
    17341 caccactatg ttgcgcccaa ctacactttc gctgagcccg agcacttctt tgaccgaacc
    17401 ggtggtgtaa agcccttcat acgacgaaag tttgataaac gtaatttcgc agggaatgtt
    17461 caccactttc atcagatcgc cggcaaacat gaaagcaccg ttaagtacac aaataaacat
    17521 tggatttttg tcggccatgt cgcggttgat ttgttcgcct acttttgaca ctgcttcctg
    17581 aataacttct gatttaattg agagactgaa ttgtttgtct ttgattgtga tggtgctcat
    17641 attgattaga gttgaaattt tttaaacatc aaaagtaaaa aaaaaaatca acacagccat
    17701 catttctata agaatattcc ctgaaaaaga aaaaaacggt gatgaataat caaaaacaac
    17761 aaaaatcaat tcagcagttt ctttttttaa aaataatctg tatatttgct aaattatttg
    17821 cgtgtaaaat tttaaaaagt aaatcaagat aaatatgaaa ttcacagtat caagtaccga
    17881 attacttagt cacctgcaag ccataagccg tgttattaac agtaaaaact cacttcagat
    17941 tctcgacaat tttctgctca gtctgcaagg aaatacgcta acaatgactg cttctgacat
    18001 tgaaactaca atgattactt ccatggaagt tcaggatgtg gaagggagtg gaactgtagc
    18061 tgtagctaac aagttgatcc tggatacgtt acgtgaattt tcggagttac ccctaacttt
    18121 ccatatcgat gattcgacgc tggccatggt aatcacttcg gcgaatggaa cttacaattt
    18181 tatcggtcaa agcggcgata tgtatccact gctgcctcag ctgggtgata atgttcagca
    18241 tttgaacgtt gccgttcctc atttgctgag cgggatttcg aaaacatttt tctgcatggc
    18301 cgaagacgag ctccgaccgg taatgaatgg catttatttt gatatttcat cagaacttac
    18361 cctcgtagcc accgatgctc ataaactggt tcgattcaaa accagctacg aaactgcttc
    18421 ggtagttgaa ggcgaaagta tgagttttat tttacctaaa aaacctgcaa ccatgctccg
    18481 gaacatattg ccgaaagaat ccggtaatgt agaaatcaag ttcgacgata aaaatgctta
    18541 tttcgaactc agcaattata ccatggtttg ccggcaagtt gaaggtcgtt atccaaatta
    18601 caacggtgta attccgaaaa acaatccatt taaaataatt attgacaggg taacgctgct
    18661 caataccctc aaacgcgtat ccgtattttc caaccaggca agcaatctga taaaactgga
    18721 ttttactcaa aatcaggtac atgtttcggc gcaggacatc gatttttcga tttcggccga
    18781 agaattcatc ccctgccagt atgagggaga accaataaaa attggattca aatcaacgtt
    18841 tttgatcgaa atgctgggta atgtggattc agcagaagta gtgctcgaac tggccgatcc
    18901 ttcgcgtgca ggaatactgc ttccggtgca ggaagatgac aacgagaaaa ttctgatgtt
    18961 gttgatgcct atgctcctca atgattaatt cttaaagaaa aaaacaataa aaatccggca
    19021 gaaaagtatc caaaaattac ttttctgctt tattttttta atagaaaaga gtgataaaat
    19081 gaaactaaac ttaaaaaatc ccattgtatt ttttgatctg gaaactaccg gtaccaatat
    19141 cgttaccgac agaatagttg aaatctccta cctgaaaatt tcacccaacg gaagagaaga
    19201 atcgaaaacc attcgtataa atcccgaaat gcacataccc gaacaagtca ctgctattca
    19261 tggaattacc gacgaggatg tggcagactg tccgcgcttt aaggaagtgg cacgtgaaat
    19321 cgcccgcgat tttgaagggt gcgatctggc gggatataat tccaaccggt tcgacatacc
    19381 gctgctcgct gaagaatttc tgagagcaga ggtggatata gacatgaaaa aacggaaatt
    19441 cgtagatgta caggtagttt ttcataaaat ggaacagcgg acattatcag cagcctacaa
    19501 gttttattgc gataaggatc tggacaatgc tcacagcgcc gaggccgata ccagagccac
    19561 ctatgaggtg ctcatggctc agctcgacag atatcccgaa ttgaaaaacg aaatcgcatt
    19621 tttgtcaggt ttcactactc aaaacaacaa tgccgacttt gccggacgaa tcatttacaa
    19681 cgaaaaagga gaagaaatca tcaattttgg aaaatataaa ggacagaaag tgagcgatgt
    19741 gctgaaaaac gacatcggct attacggatg gatcatgagc agcgacttta cacttgacac
    19801 caaaaatgtt cttaccaaaa tcaaactccg ggattttcaa aaataaatac tttcctgctt
    19861 cgggaattga tacaacgggc agattttttt tcttacaaaa agtttagaaa gttaaaaaac
    19921 gcatttacaa tatcctatat taaatattgg cacagtcatt gttgctcatt cagtaacgat
    19981 ttacaaatca tgatatttgg tgaagttgaa ctatgagaag agttttgaaa tacatattgt
    20041 ttttattact ggcaataaat atacctacct tttctgttgc tcaaagtacc tccgtgatcg
    20101 acaggacacc ggaacaggaa gctgtgaagc aaactgaaaa aatgcagaaa gagctgaatc
    20161 tcacacctga acaggtgaaa aacatctacg agatcaatct taaatatgcc cgcgaacgca
    20221 agcaatccaa taaacgctcc gatgctgtaa accgcatcag atcgaaaact gaagaaatcg
    20281 gtaaggtgct gaacgatcgt cagaattatg aattacagac aaaacgatcg gaagtccaat
    20341 cggtagaaat agatggtaaa aggcaatcaa cccgaactga tgccaataca aggtttaatt
    20401 cgggtcaaaa tagcgaaaca aatacacgaa cacaattccg ggaccgaccg gtaaggactg
    20461 accggtctca acgacaaata caaactcctg cttcggacag atacagatca acgagcgaca
    20521 aaatggaaaa tcctgtacga agaaatgaca gcagaacgga ggttggtcgt caaaccaggg
    20581 atgttcctta cagtgtcggt ggcagcagtt catcttcgcg aagtgtacaa caaggttcta
    20641 cccgcagttc ttcatccgaa agccggagtt catcgcagcc tgcaagccgt gagtccggaa
    20701 gcagaagata agaaaccgta catgtcaaat ccctttttta aattcaaaca attcactgtt
    20761 tttcatgaca agtgtgccat gaaagtaggt gttgacggag ttacacttgg tgcctgggca
    20821 gatgtttcgg atgtaaagtc catattggac gtcggatgtg gttccggtct gattactctg
    20881 atgctcgctc agcgatgcga agcccgtata acagccatcg atattgataa acaaagcatt
    20941 gtacagacca gagaaaatgt aggaaattca ccctgggaca accgcatcag ggttattcac
    21001 acttcctttc agcaatttgc atccggttcg tcagccggtt ttgatctaat cgtatgtaat
    21061 ccgccgtttt ttatcaattc gatgaaatcg ccttccgaat cgagaacaaa agcacgtcat
    21121 tcagattcac tgccattctt agaactgatt ttaaatgcaa aaaaaatgat gactgaaaaa
    21181 ggacggctat gtgtcattct gcccgtgccc gaaggaaaaa ttttcaacga actcgctgta
    21241 aaggaaaaac ttttttgcag caggctggtg aatgtatttc caaaccccga aaaacctgct
    21301 aaacggcttt tgatggagta tcaaaaggaa gtgacagatt gtaaaaccga agaactgatt
    21361 atcgagaaag aaagaaatct ttatacaccg gaatactcag ctttggtaaa agatttttac
    21421 ctgaataaaa actgaaattc aggatttttt atctttctta tttctttcca cttttttcca
    21481 cagaaatttt gcagccagca cactgcccag agttcctccg gcagcaatga tgaaagcttc
    21541 aagaccaagt tt
//
LOCUS       DAORKP010000019        20908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_113_length_20908_cov_8.561502, whole genome shotgun sequence.
ACCESSION   DAORKP010000019 DAORKP010000000
VERSION     DAORKP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20908
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113_length_20908_cov_8.561502"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLN75_02170"
     CDS             complement(<1..938)
                     /locus_tag="PLN75_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02953.1"
                     /translation="MKFHKLLLIGLISPLFLFSQELKWNTELNFFFDNNEFAKSPLAI
                     DQTMAGVTLTQEIGWKFDDKHYIVGGVDAMRSLGADKLVNDIHLLAYYQLKDENTLLK
                     VGSFHRHDLLDDYSDFFFQDSIKYYRHTMEGMYFRKGNENQFVKLWLDWTGLQSATER
                     ETFFIGASGYKKFGKLFFADFQSYMFHYSITRPNPRGLTVSDYLLGQLSGGVNYSNRS
                     GINKLLVSVGLLSGFERDRVDMNNYGTPTGGVARVEFDYRGFGTENTLYYGQPRMVLY
                     YRHANNLYWGNPFLRAESYMQNKVYWNFINNDHIKG"
     gene            complement(1014..1775)
                     /locus_tag="PLN75_02175"
     CDS             complement(1014..1775)
                     /locus_tag="PLN75_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRG02954.1"
                     /translation="MDKIWLNALISTIAVSIISLIGVSLFALKTEKLQKILLLLVCFS
                     VGALLGNAFFHLVPESYFHIESPKLTSWLIIAGFMLFFLVEQLLHTHQAANPAVSFRV
                     KPYGYLSLYADGIHNFTDGILIGAAWMFSPELGLATTITVILHEIPQEISDFGILLKA
                     GFSKKKALFYNFLVATSAILGTLLALWFGHEIEHFSVYVLPVAAGGFIYLAATSLLPE
                     VLKNTAKGKWFIHSVFVLLGLALMYYFSLTGGHHH"
     gene            complement(1992..2474)
                     /gene="ispF"
                     /locus_tag="PLN75_02180"
     CDS             complement(1992..2474)
                     /gene="ispF"
                     /locus_tag="PLN75_02180"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRG02955.1"
                     /translation="MKLRVGFGYDVHAFAAERELWLGGIKLDYQPGLLGHSDADVLIH
                     ALCDALLGAANMRDIGFHFPDNASEFKNIDSKILLHHTMLLIREKGWEFGNADCTVVA
                     EQPKLNPYIPAMQKTLAEVMQVNVDDISIKATTNEKMGFVGRQEGMAAYATVLLQKQD
                     "
     gene            complement(2594..3769)
                     /gene="porV"
                     /locus_tag="PLN75_02185"
     CDS             complement(2594..3769)
                     /gene="porV"
                     /locus_tag="PLN75_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRG02956.1"
                     /translation="MKKTFLVLLTTFLISLSVFAQVEPNEQLNPLITGVPSLTITPDS
                     RGGGMGDVGAATTPDINSQHWNPAKYAFMESPAGFSFSYTPWLSKLVSDINLLYAAGY
                     WKFDEQQSVSASLRFFSLGEIKLTGERPEDDYGTAHPNELAFDLAYSRLLSEKWSAAV
                     AFRYIRSDLNNGINNSADPMYPGNAIAADVATYYRTPIEMSTGDANLAFGLNISNIGS
                     KISYDQNLTSIFIPTNLKLGGSFDYPFDDYNKISISADINKLLVPTPIKRLEGESDTD
                     YEARRTSYYETGPIAGIFKSFGDAPGGSSEELKEIMWSVGAEYAYNNQFFVRGGYFNE
                     NAMKGNRKYFTAGVGFKLNMFQLDAAYLISAAQSNPLDQTLRFTLGFDLNGLQNLMR"
     gene            complement(3865..7668)
                     /gene="porU"
                     /locus_tag="PLN75_02190"
     CDS             complement(3865..7668)
                     /gene="porU"
                     /locus_tag="PLN75_02190"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRG02957.1"
                     /translation="MKFKFLITIALITCVAFASTALNPFVSKYEIKWSRVETWKAGTH
                     VKQVLSFDQAQYPSETDLPHFAERIIADENAGYQVSVENAEFIPLTAEEEKIVARNAI
                     PQTIEVNTFNGYERGTTYLNIQILPFVMKDNKVMKVSRFNLQIVKQPKPSRIFSPVMH
                     SYAASSVLASGKFVKVKIKETGIYKLTYEALNSMGVNSANVRVFGYGGALLDQDFTKP
                     KPDDLPEVAVWMEKGADGVFNAGDYILFYGQGLVKWRYDQPRSMFTHTLNHYANEGYY
                     FITSDAGQGKKIQDAAITVPAGTEVAEVTEFTDYQLHEVEKYNLAKTGKVFYGEIFEN
                     TTSYNFSFDFPNMAAGNVKARVDVAATSTETSGFVLKLNGAQELSFSVSRNSGDNYEI
                     GKEANATLSFPVTNPNSKAEFTLTYSKPNLSARGYLNYLEVNARRKLVMNGAVLFFRN
                     TDNLNSFVYNRYRISGVNANVQVWDITDPANVKRIVAARSGDVLEFTDSGETLKEYLA
                     IDPTAKASFTLEPTPAGTVANQDIHGMQAAEMVIITHPTFVPQAEKLANAHRQMDNLR
                     VNVMTTDQVYNEFSSGTPDASAYRWAMKMFYDRALAAADASAMPKYLLLFGRGSYDNR
                     GIINNSGDNLVITYQADMSLDIVKSYVTDDYFGFLDDKEGLYPPANLLDIGIGRFPVA
                     TSQQADDVVNKTIAYMKNEIRGNWKNQLCFLADDEDGALHMRQADSIAQSLKKNNQGF
                     QFNKIYLDAYLQETSASGDTYPLATTKLHNLINTGLLMLNFTGHASENGWTKEKVLLA
                     DDVKNMYNTKLPLWVAATCDFVLFDVKNISAGEYVLLNPAGGGIALFSAARTVYASQN
                     ERLNRFFTLDLFEKNNGKYPRLGDAVFKSKNQTGTEINKLSYMLFGDPALRLTYPTGY
                     RVITEKINDKTVSNSDTLKALSVNKFQGYIVDDNNNKVTDFNGKLEISIYDKMQKITT
                     LDNDKAGSITYEDRPNLLYSGKANITDGVFTFTAMIPRDIRYNYGSGRINYYASDSNS
                     DREAQGFYENFTVGGSTPDFIQDNEGPAVTMYLNTPNFTPAGKVNETPMFVARVSDKN
                     GINTVGSGIGHDMRLVIDGNPLTSYTLNDNFEAETGSYQEGTVRMKLPQMSAGKHTIT
                     FFCWDLLNNSTTATLDFEVVPGLIPELFSVYNYPNPVRTGTSFVIEHDRPQVILETTL
                     DIFDISGRLIISVRQNSAENLKWDLKDSTGKKVQAGVYLYRISIKTNNSEQTSKANKI
                     IVLGQ"
     gene            complement(7750..8361)
                     /locus_tag="PLN75_02195"
     CDS             complement(7750..8361)
                     /locus_tag="PLN75_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRG02958.1"
                     /translation="MKIFAIGQNYTEHNKELDNANPTEPVVFMKPDTAVLRNNKPFYI
                     PDFSDEVHYETELLVKINRIGKNIAPKFANRYYDEIGLGVDFTARDIQRRLKKEGKPW
                     EISKAFDNSAVIGDFIPKSELGNLQNIHFHLDMDGQTVQQGNSADMIFEIDTLIAYVS
                     RFFTLKIGDILFTGTPAGVGRVSIGNRLEGYIFNKKMFDFYVK"
     gene            complement(8363..9019)
                     /locus_tag="PLN75_02200"
     CDS             complement(8363..9019)
                     /locus_tag="PLN75_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRG02959.1"
                     /translation="MPQTIYNVPEPTLRRLPWYLAYAQLVQHSGETFLSSTQIAKNIA
                     VDASKVAKDLSYVQISGRTRVGYDVNELVEVLEKFLGFTKSHKAFLFGVGSLGGALMH
                     DNGLNHFGLEIVTGFDVKYELAGTSINRIPIHHVDRFKELQKQTGINIGVLTVPVERA
                     QETADMMIEGGIQAIWNFTPFRIRVPENIIVQNTSIYAHLAVMFNRLNELSENEKNRK
                     "
     gene            9267..11855
                     /gene="clpB"
                     /locus_tag="PLN75_02205"
     CDS             9267..11855
                     /gene="clpB"
                     /locus_tag="PLN75_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRG02960.1"
                     /translation="MNFNNFTTKSQEAVQQAIDLAQAKSQQIIEPAHLLMGVMMKGED
                     VVQFLFGKTGIQPSVIQSAVEQIIAGYPRVSGGEPYFSRETNEVLQKAIDYSSRAGDQ
                     FVTLEYILFALLHVNSQVSKLLKDAGATEKDLQTAINELRKGAKASSSSAEETYNSLN
                     KYAINLNEQARAGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIVRGDVPENLKSKQVFSLDMGALVAGAKYKGEFEERLKSVVNEVIRSEGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQIV
                     MVNEPDIQSAISILRGLKERYENHHKVRIKDDAIIAAVELSNRYITDRYLPDKAIDLM
                     DESAAKLRMEVDSVPEDLDKIQRNIKQLEIEREAIKRENDVKKLENLNAEIANLKEES
                     SILKAKWSSEKALVEKIQQTKIEIENLKYEAEKAEREGDYGKVAEIRYGKIKSAEEQI
                     TQFQQELVAIQGNNAMIKQEVDNEDIADVVSRWTGIPVSKMLQSEKSKLLHLEDELHK
                     RVVGQDEAIAAVSDAVRRSRAGLNDPRRPIGSFIFLGTTGVGKTELAKALADYLFDDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYEEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDNKGRVVNFKNTIIVMTSNIGSQMIRENFERINDLNHDEIVE
                     QTKNEVMNLLKKVVRPEFLNRIDEIIMFAPLNEKQIDEIVKIQLNNISNMLADNGVRL
                     QVTERALEFIAREGYDPQFGARPVKRAIQRYILNDLSKEIISGKLDNEKVITVDYRDN
                     KLVFTN"
     gene            complement(12038..12415)
                     /locus_tag="PLN75_02210"
     CDS             complement(12038..12415)
                     /locus_tag="PLN75_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02961.1"
                     /translation="MRKFLIILTVFFLVFSLIFVILPMGTIALLPTGLALAGGILAFV
                     KSEPALRKMPKWLMITAIALFVVALGKVIFVKNEAENDQVFLQEQIQSTEEAQQELEE
                     LDEMGNTDQASDAQSTVAPDSIQ"
     gene            complement(12431..13183)
                     /locus_tag="PLN75_02215"
     CDS             complement(12431..13183)
                     /locus_tag="PLN75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRG02962.1"
                     /translation="MKKFFLIIAVFIFTGISNAQNIKNEKFVYVGSYNMSGLMTQFAQ
                     ITIQTKPITTSKKTFIHSSLIATTYTKWDTFFKIRDLYESYVDPVTYKPSMYKRNTLE
                     GNYRKTEKYVFSNGKVTSTSTRMGSPEVTKTFPVGASTNDIVTAVLRLRKMDFNKIRA
                     GQIITFVIAFDNKEFPASVKFMGKETLKNLGNLGTKECYKLSIAANTKALRGNDKNVF
                     WMTADTNRIPVLIQFSIPVGTGQVKLTNATVN"
     gene            complement(13195..14178)
                     /locus_tag="PLN75_02220"
     CDS             complement(13195..14178)
                     /locus_tag="PLN75_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02963.1"
                     /translation="MDDKNSKEMNLIELLNLLGKWIKKVTARLIRFVGFLLQLAFKHW
                     VVSAIVLLLSVVAGQYLARPSVRRYEANAMAMLYGCENSTVKEVFRQLQNSLIPKKST
                     TFSSMNLATVLGIPDSVAENIVKLESYNVIDYLDDNTPDMVDFDNSHSLKDTMNLIMK
                     NHIFIQVQTLKPTQIRIFQDALLNYFNNNPMLKSEFESTKTGIENKIRLCDTEIQRLD
                     SLANLSYFKDSDKKISLENNNLIVGEQRKQLFYNDILSLQELRNINTNSLVLYKKPVD
                     FPAGLIVNPVPINGRIKYGVYSIFIGSLLALIMVYLIDNLKKIINYLINKK"
     gene            complement(14200..14880)
                     /locus_tag="PLN75_02225"
     CDS             complement(14200..14880)
                     /locus_tag="PLN75_02225"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRG02964.1"
                     /translation="MESGVHEKKKIYYAVFFPLIICFFVILVFVFERAMGIDFSRGGV
                     FPGEPKTIINIFSMPFIHADVTHLLNNIVSFFVLSTTLYYFYSEIANKIVVLSVIISG
                     LLLWVIGRDNWHIGLSGVIYSLTFFLFFSGIIRKHVPLIAIALIVVFLYGNNVWHLFP
                     WQKFDPVSWEGHLAGAVSGTLLAFAYRKQGPQKPVKVWEEDDDNDNELSDMAEEDETE
                     NDELQQFN"
     gene            15111..17063
                     /locus_tag="PLN75_02230"
     CDS             15111..17063
                     /locus_tag="PLN75_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRG02965.1"
                     /translation="MSYLNFDKTLLMNLEKSLSKEMIRTNRAGTYNSTTLVDCNTRKY
                     HGQLVMPLPDIDNSNHVLLSSLDETVIQHGAEFNLGVHKYSGNNYNPNGHKYIRQFDC
                     ETISRTIYRVGGVILSKEKMLVSFEPRILIKYTLLEAHSPTTIRFKPFLAFRSVNELT
                     YQNNVANTSYKEVKNGISMGLYEGYPELYMQFSKKNTYFHMPDWYKGIEYTKEQERGF
                     EFKEDLLVPGYFEMPIKKGDSIIFSGGISEISPRSLDSLWEKEILHRNHRLDMFTTLK
                     NSAQQFYKRVGDKTYLLAGYPWFGSRARDEFVSLIPTTLIIGREDYFDTIAQTAIEEI
                     EKFLDGAREDFKLEGLEDPDVFLWFIRAVQQYAEFRSPEQAAERFGDLVIRIINYIKK
                     QNHPNLFMHNNNLLYVNGTEKPATWMNAVENSHPITPRTGYVVEINALWYNALKFVST
                     LERLSGKEQSADLLDYQAEIARDSFVKVFWNGTYLYDYVVDDYRDMEVRSNMIIAAGL
                     PFSPLDKQQRKSVVDITTKELLTPKGLRSLSPKSGSYRPEYIGGMLERSWNYHNGPVW
                     PWTVGYFTDAYLRIYKQSGKSFIERLMTGLEAEMTELCVGTISELFDGNPPFKGHGGM
                     SFAMSVAEILRVLDKLKKIENEIPAL"
     gene            17060..18319
                     /locus_tag="PLN75_02235"
     CDS             17060..18319
                     /locus_tag="PLN75_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRG02966.1"
                     /translation="MKALMFGWEFPPHILGGLGTASYGLTRGMSEQPDMEITFVIPKP
                     HGDEDQSFLKIIGACNVPVVWKENSWEHVNNRIGKIMHPDEYFWLRNGIKYDFRRIYT
                     NDLGCIGFSGKYPDNLIEEISNYEAVASVLAHQLDFDVIHSHDWLTYPAGRFAKQISG
                     KPLVIHVHATDFDRSRGKVNPTVYKIEKDGMDAADHIMTVSDLTRRTVIEKYHQHPNK
                     VTTVHNAVEPLPDVDSFVKTPRKDKIVTFLGRITMQKGPEYFVEAAARVLSKTKNVRF
                     VMAGSGDMMNAMIDLAAKRGIADRFHFPGFLRGRDVNEMLAQSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISYQSGCAEILTHVVKTDYWDIDAMADAIYGIVTYPAMYKSMKELG
                     LEEVNNIKWYDAGLKVRAIYDKVIRKH"
     gene            18414..19697
                     /locus_tag="PLN75_02240"
     CDS             18414..19697
                     /locus_tag="PLN75_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRG02967.1"
                     /translation="MKNICFYFEIHQPLRLRRYRFFEIGQDHYYFDDFQTEDRIRGMV
                     EQAYLPAIRTIAEMIRSSNGKFKCSFSISGVAMEQLEQYAPEVIDGFRDLAKTGSVEF
                     LAEPYAHSLASVYNRSEFEQQVKLHSKKIEALFEKKPTVFRNSELIYSDEIGEIVSAL
                     GFKTMLIEEVKYVMGWKSPNFVYNHSYIPKLKLLVRNNKLSDDIAFRFSDHSWSDFPL
                     SAEKFIRWISELPKSDPVVNLWMGFEAFGFYQRAESGIFEFLKALPYHAMEQGHSFAL
                     PSEITKKFDSVDSLTSPYPTSWSGEKDLSVWNGNDLQQEALNKLYAVSERVHLCKDAP
                     LKNDWLKLQTTDNFRYMSHFDAFGTHYTSPYEAFTNYMNVLADFLDRVDAQYPTTIEN
                     EELNELLKTIISQESEIATLEEELKKARARKKKEE"
     gene            complement(19834..20469)
                     /locus_tag="PLN75_02245"
     CDS             complement(19834..20469)
                     /locus_tag="PLN75_02245"
                     /inference="COORDINATES: protein motif:HMM:NF027595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4923 family protein"
                     /protein_id="HRG02968.1"
                     /translation="MKFRNLFFLLVVSSAVVFSSCGLLSGTSDAKSENKKTGSGNVLE
                     DIFNTVMGKLPITQQSMVGTWNFQGTSCAFETENLLKKAGGAVVATQVEDKFDETCTK
                     LGISEKNTGFVFHADSTYTGRIGGMKISGKYMLNPESKQVQMTYLLGVGKLKATVAVS
                     SREMKLLFDADSFLKLMKIISQFTNDASIEVLGKMADMYDGMLLGFDMRKQ"
BASE COUNT         6162 a         4545 c         4382 g         5819 t
ORIGIN      
        1 tgacctttaa tatgatcatt atttatgaaa ttccagtaca ctttattctg catgtagctt
       61 tctgcacgga gaaacggatt tccccagtac agattattgg catggcggta atacaatacc
      121 atgcgtggtt gtccgtaata gagggtgttt tcggttccga agccacgata gtcgaattcc
      181 acacgggcta cgcctccggt aggtgtaccg tagttgttca tatctactct gtcacgttca
      241 aagcctgaaa gcagtcccac agaaacgagc aatttattga taccgcttcg gttggaatag
      301 ttgactccac ccgacaactg accgagcaga taatccgaaa ccgttagtcc ccgcggattg
      361 gggcgggtaa tggaataatg aaacatatag gactgaaaat cggcaaaaaa caattttccg
      421 aattttttgt aacccgaagc gccaatgaag aaagtctcac gctcagttgc actttgcaat
      481 ccggtccagt ccagccagag tttaacgaac tgattttcgt ttcctttcct gaaatacatg
      541 ccttccatgg tgtgtctgta atactttatg gaatcctgaa agaaaaaatc ggaataatcg
      601 tcgagcaaat cgtgacgatg gaatgatcct actttcaaca gtgtattttc atctttcagc
      661 tgataatatg ccagcaaatg aatatcgttt acaagtttat cggctcccag acttctcata
      721 gcatctacac ctcccacaat gtaatgcttg tcatcaaact tccagccaat ttcctgagtg
      781 agtgttacac cggccatcgt ctgatcaata gccagtggcg actttgcaaa ttcattgtta
      841 tcaaagaaaa agttcagttc cgtattccat ttcaactcct gtgagaacaa aaacagcggt
      901 gaaataaggc cgataagtaa taatttgtgg aatttcataa ttctatacga ttgattattt
      961 attcagataa aaaatttaaa ggaatcatta gaaaggtact tactgtgtcc actctaatga
     1021 tgatgccctc cggtaaggct aaaatagtac attagtgcta atccaagtaa aacaaaaaca
     1081 ctgtgaatga accatttgcc tttcgctgta ttcttgagca cttcaggcaa taaactggtg
     1141 gcagccaggt agataaatcc tcctgcagct acgggcagca cataaacgga gaaatgctct
     1201 atttcatgcc caaaccacaa tgcaagcaat gtacccagaa tggccgatgt agctaccaga
     1261 aaattgtaga aaagtgcttt tttcttcgaa aaaccggctt tcaacaggat tccgaaatca
     1321 cttatttcct gcggaatttc gtggagaatt accgtgatag tggtagccag acccagttcg
     1381 ggcgaaaaca tccaggcagc gcctatcaga attccatcgg taaaattatg aatcccgtcg
     1441 gcgtaaaggc tcagatagcc gtaaggtttt accctgaacg aaacagccgg attggctgcc
     1501 tgatgcgtgt ggagcaactg ctcgaccaga aaaaacagca taaaaccggc aataatcagc
     1561 caggaagtca gtttgggaga ttcaatatga aaatacgatt cgggtaccag atgaaaaaaa
     1621 gcatttccga gcaatgctcc taccgaaaaa caaaccagca gaagcagtat tttctgcagt
     1681 ttttctgttt ttaatgcaaa taatgaaaca cctatcagcg aaatgatgct tacagctatt
     1741 gtacttatca gggcattcaa ccagatttta tccatttttt tttatttaac ttacaaaagt
     1801 atgtaaaaat tatcaaaatt agttcaaata tcagcaaaaa agactgtctg agttttcagt
     1861 acagtcttca tcagggtgat tacagcacat ttataaagaa ccggaaaaaa acgttttaac
     1921 ttcccgatgt cagttttttc aaatgtatat cttgcttttc ctttaaaatc aaatcagtat
     1981 ccggatggaa attaatcttg tttttgaagt aaaacggtag cataagctgc cataccttcc
     2041 tgtctgccga cgaatcccat tttttcgttt gtagtagctt tgattgaaat atcatccaca
     2101 ttgacctgca tcacttcggc gagtgttttt tgcatagcag gaatatacgg attcagcttt
     2161 ggctgctcag ccactacggt gcagtcggcg ttgccaaatt cccatccttt ttccctgatg
     2221 agcagcatgg tatggtgtaa aaggatttta ctgtctatat ttttgaattc ggaggcattg
     2281 tccgggaaat gaaaaccgat atcgcgcatg ttggccgctc ccagtagtgc atcgcaaagt
     2341 gcgtgaataa gcacatcggc gtcagaatgt cccagcaaac ccggctgata atccaacttg
     2401 attccaccca gccaaagttc gcgttcggca gcaaacgcat gtacatcata tccaaatcct
     2461 actctaagtt tcataaggta aatatcttta tttcaaaagt aaaattgcat tgcaaccaaa
     2521 aacagtatat aaaacaaaaa agactgtagc tggttcaagg caaacagtct ttttgaagaa
     2581 aaaaaagtat gcattaacgc atcagattct gtaaaccatt caggtcgaaa ccgagcgtaa
     2641 agcggagtgt ttggtccagc ggattggatt gtgctgcaga aatcagataa gcagcatcaa
     2701 gctggaacat gttgagtttg aatccgacgc cggcagtgaa gtatttgcgg tttcctttca
     2761 tggcgttctc attaaaatat ccgccacgta cgaaaaactg attgttgtat gcgtattcgg
     2821 ccccaaccga ccacatgatt tcctttagtt cttcggaaga tccaccggga gcatcgccaa
     2881 acgatttgaa aatacctgca atgggtcctg tttcgtagta ggaagtacgg cgggcctcat
     2941 aatcggtatc gctctcgcct tcgagccttt tgatgggagt aggaaccagc agtttgttaa
     3001 tgtctgcact gatggatatt ttgttgtaat catcaaaagg ataatcaaac gatccaccaa
     3061 gtttcagatt agtagggata aaaatgctgg tcagattctg atcatacgag attttggaac
     3121 cgatattgga gatattcaat ccaaaagcga gattggcatc gccagtcgac atttcgatgg
     3181 gagtgcggta atatgtggcc acatcggctg caatagcatt tccgggatac atcggatctg
     3241 ccgaattgtt gattccgttg ttcaagtccg aacggatata cctgaaagca acagcagccg
     3301 accatttttc tgaaagcaga cgggagtaag ccaggtcaaa agccagttcg ttcggatggg
     3361 cagtaccgta gtcatcttcg ggtctttcac cggtgagttt gatttcacca agggagaaaa
     3421 agcgaagaga tgcacttacc gattgctgtt cgtcaaactt ccagtaaccg gctgcgtaga
     3481 gcaaattaat atcgctcacg agtttactca gccagggagt atatgagaac gagaaaccgg
     3541 ccgggctctc cataaaagcg tatttggcag gattccagtg ttgagaatta atatccggag
     3601 tggtagcagc tcccacatcg cccataccgc ctccacgtga atcgggagta atggttaacg
     3661 aaggaacgcc agtaatcagc ggattcagtt gttcgtttgg ctcaacctga gcaaataccg
     3721 ataatgagat aagaaaagtg gttagtaata ctaaaaaagt ctttttcatt ctttatgatg
     3781 gtttaaaatt tatctgtatt gtttttttgt cctgtttttt tatgccattg ctggattaat
     3841 aatttaaact catgaaaaat tattttattg cccgagcacg attattttat tcgcttttga
     3901 agtttgctca ctgttgtttg ttttgatcga gatgcggtac agatatacac cggcttgcac
     3961 ctttttaccg gtgctgtctt tcaaatccca tttaaggttt tctgcactgt tttgtcttac
     4021 agaaatgatc agcctgcccg aaatatcgaa aatatccagt gtggtttcca gtatcacctg
     4081 cggacggtcg tgttctatta cgaaactggt acccgttcga accggattag gataattata
     4141 cacactgaat aactcgggaa tcagtccggg cacaacttca aaatctaacg ttgccgtagt
     4201 tgaattgttc agcaaatccc agcaaaagaa ggtaattgtg tgttttccgg cggacatctg
     4261 aggcagtttc atacgtacgg taccttcctg gtagctgcct gtctcggctt caaaattatc
     4321 gttcagcgta taggaagtca gcggattacc gtcgattacc aacctcatat cgtgtccgat
     4381 accgctacct accgtattga ttccgttttt atccgaaact cgggctacaa acatcggagt
     4441 ttcattcact tttccggccg gagtgaagtt gggtgtgttt agatacattg taactgccgg
     4501 accttcgtta tcctgaataa aatccggagt tgatcctcct accgtgaaat tttcataaaa
     4561 tccctgagct tctctgtctg aatttgaatc agatgcatag tagtttattc tccccgaacc
     4621 gtaattatag cgtatgtcac gtggaatcat ggccgtaaaa gtaaacactc cgtcggtgat
     4681 atttgctttt ccggagtaaa gcaaattggg tctgtcttca taagtaatgc ttcccgcctt
     4741 atcgttgtcc agcgtggtaa ttttctgcat tttgtcgtaa atggatatct caagtttacc
     4801 gttaaagtct gttaccttgt tattgttgtc gtcaacaatg tatccctgaa acttattgac
     4861 agaaagcgct ttgagcgtat cggagtttga tactgtttta tcgttgattt tttcggtgat
     4921 aaccctgtat ccggttggat aagtgagtct taaagcagga tcgccaaaga gcatgtacga
     4981 caatttatta atttcggtac ctgtctggtt ttttgatttg aatacagcat cgcccaggcg
     5041 gggatatttt ccattgtttt tctcaaaaag gtccagtgtg aaaaaccggt tcagtctttc
     5101 gttttgcgaa gcgtaaaccg tgcgtgctgc tgagaaaagc gcaattccac ctcctgcagg
     5161 attcaggagt acgtattcac cggccgaaat atttttgacg tcgaacagca cgaaatcgca
     5221 ggtagcagct acccacaagg gcaatttggt gttatacatg tttttcacat cgtcggccag
     5281 caatactttt tcttttgtcc agccattttc actggcatgt ccggtgaaat tgagcatcag
     5341 cagccccgta ttgatcaggt tatgcagttt tgtagtagcc agtggatagg tatcgccgct
     5401 ggccgaggtt tcctgaaggt atgcatcaag gtaaatttta ttaaactgaa atccctgatt
     5461 attctttttt agtgattgcg caatactatc agcctgacgc atgtgaagcg ctccgtcttc
     5521 atcgtcggca agaaaacaga gctggttttt ccagtttccc cgaatttcat ttttcatgta
     5581 tgcaatggtt ttattcacca catcatcggc ctgctggctt gttgccaccg gaaatcgtcc
     5641 gatgccgata tcaagcaggt tggccggagg atacagccct tccttgtcgt ccagaaaacc
     5701 aaaataatca tcggttacat aagactttac gatatccagt gacatatctg cctgataggt
     5761 aatgaccagg ttgtcgcctg agttatttat tattccgcga ttgtcgtacg agcctctccc
     5821 gaagagcaac aggtactttg gcatggctga agcatccgct gcggctaatg ccctgtcgta
     5881 aaacattttc attgcccacc ggtaggccga agcatcgggt gtaccggatg aaaattcgtt
     5941 gtacacctga tccgttgtca tcacattgac tctgagatta tccatctgcc ggtgtgcatt
     6001 ggccagtttt tcggcctgag gcacgaaagt tggatgtgtg attatgacca tttcggcagc
     6061 ctgcataccg tgaatatcct gattggctac agttcctgcc ggtgtgggct caagtgtaaa
     6121 tgaagctttg gcagtggggt caatggcaag atattctttc agcgtttcgc cagaatcggt
     6181 aaattcgagt acatctccgc ttcgggcagc tacgattcgt ttcacgttag ccggatcggt
     6241 tatatcccac acctgaacgt tggcattgac tcccgaaatc cggtaccggt tgtaaacgaa
     6301 tgaattcagg ttatcggtat ttctgaaaaa cagcaccgcc ccattcatca ccagtttccg
     6361 gcgggcattc acttccagat aattgagata tcctctggcc gataagttgg gtttggaata
     6421 cgttaaggta aattcagctt ttgaattcgg attggtaacc gggaaactaa gtgtggcgtt
     6481 agcttcttta cctatttcgt aattgtctcc gctatttctg gaaactgaaa aagataattc
     6541 ctgcgctccg ttgagtttca gaacgaaacc ggatgtttcg gtcgaagtgg ctgctacatc
     6601 tactcttgcc tttacattgc cggcagccat gttcggaaaa tcaaaactaa aattatagga
     6661 agttgtattc tcaaaaattt ctccgtaaaa tacctttccg gtttttgcca ggttgtattt
     6721 ttccacttca tgaagctgat aatccgtaaa ttcagtcact tcagccactt cggttcctgc
     6781 aggaactgta atggcagcat cctgaatttt cttaccctgg cctgcatctg aagtaatgaa
     6841 gtaatatcct tcgttggcat aatgattgag ggtatgggta aacatggatc tgggctgatc
     6901 atacctccac tttaccaaac cctgaccgta gaagaggatg taatcacctg cgttaaacac
     6961 accatcggcc cccttttcca tccacacggc cacttcaggc agatcatccg gttttggctt
     7021 tgtgaaatcc tgatcaagca aggcaccgcc gtaaccgaaa accctgacat tagccgaatt
     7081 tactcccatc gaattaaggg cctcgtaggt tagtttatat attccggttt ctttaatttt
     7141 tacttttacg aattttcccg atgctaaaac agaagatgct gcatacgagt gcattacagg
     7201 tgagaaaatt cgggatggtt ttggttgttt tacgatctga agattaaatc tactcacttt
     7261 catcacctta ttatctttca ttacgaaagg caaaatttgg atattcagat aagttgtccc
     7321 ccgctcgtat ccattgaatg tattcacctc aatggtctgc ggaatggcat ttcgggcaac
     7381 tattttttct tcttcggctg taagcggtat aaactccgca ttttcaaccg atacctggta
     7441 gccggcattt tcatcagcta ttattctctc tgcaaagtgc ggaagatccg tttcggaagg
     7501 atattgagcc tgatcaaaag ataatacctg cttcacatgt gtacctgctt tccaggtttc
     7561 aacgcgtgac cattttattt catattttga gacaaaagga ttcagggcgg tggatgcaaa
     7621 ggcaacacat gtgattaaag ctatggtaat gagaaattta aacttcatag tagggtgaat
     7681 aaaaaaacga ataaaaagct aacatctata acgacaatca tgtgtattta ttattgcgca
     7741 gtcagcttct tacttcacgt aaaaatcgaa catttttttg ttgaaaatgt aaccttccag
     7801 cctgttgcca atgctcactc tgcctactcc ggcaggagta cccgtaaaga gtatgtcgcc
     7861 aattttcaat gtaaaaaaac ggctcacgta agcaatcagt gtatctattt caaaaatcat
     7921 gtcggctgaa tttccctgtt gtacagtctg gccgtccata tcgaggtgaa agtggatatt
     7981 ctgcagattg cctaattcac ttttgggtat gaaatcgcca ataacagccg aattatcaaa
     8041 cgctttggat atttcccacg gtttgccctc tttcttgagc cgtcgctgaa tgtcgcgtgc
     8101 tgtaaaatca actcccaggc ctatttcatc gtagtatctg ttggcaaatt taggtgcaat
     8161 atttttcccg attcggttta ttttcactaa taactctgtt tcgtaatgca cttcatcact
     8221 gaaatccggg atataaaacg gtttattgtt gcgcagcacg gcagtatcgg gtttcatgaa
     8281 aactacgggc tcggttggat tagcgttgtc caattccttg ttgtgttcag tgtaattctg
     8341 acctattgca aaaattttca tattacttcc ggtttttttc gttttcgctc agttcgttaa
     8401 gtctgttgaa catgactgcc agatgcgcat agatggatgt attctgcaca ataatattct
     8461 ccggcacccg aatgcggaaa ggggtaaaat tccaaatggc ctgaatgccg ccttctatca
     8521 tcatgtcggc tgtttcctgc gctctttcca ccggtaccgt cagcacacct atgttaatac
     8581 ctgtttgttt ctgcagttct ttgaaacgat ccacgtggtg aatggggatt cgattgatgc
     8641 ttgtaccggc cagttcatat tttacgtcaa aacctgtcac tatttccaac ccaaaatgat
     8701 tcaacccatt atcatgcatc agagcacctc ccagacttcc cacaccaaag agaaatgcct
     8761 tgtgcgattt tgtaaatccc agaaatttct caagcacctc caccagttcg ttcacatcgt
     8821 atcctacacg cgttcgtccg gatatttgca cgtaagacaa atctttagcc accttcgatg
     8881 cgtccacagc tatatttttt gctatctgtg ttgacgatag aaaagtttcg cccgagtgct
     8941 gtacaagctg cgcataagcc agataccagg gcaatcgccg gagagttggt tccggaacat
     9001 tgtatattgt ctgtggcatt atttggttta ttttcaagat ttaaactaca aaattaatta
     9061 ttttttttgt aaaaatatca gttttattcc gcttcataca ccaaacaaac cttaaaatat
     9121 taaaaataac aattttgcca ggaaaaatga aattttgtca tacaaaacaa cttttactcg
     9181 gaaatttctg gtcattttgt ccgattatca gaatggtaag ctatttgaag catatcgaat
     9241 aaaattaatt cataaaaaaa ccacatatga acttcaataa ttttaccaca aaatcgcagg
     9301 aagccgttca gcaggcaatt gaccttgctc aggcaaaatc gcagcaaatc atcgaaccag
     9361 ctcacctcct gatgggtgta atgatgaaag gtgaagatgt cgttcagttt ttgtttggaa
     9421 aaacgggcat tcagccgtct gttattcaat cggctgtaga gcagattatt gcaggctatc
     9481 cgcgcgtttc gggaggagaa ccgtattttt cacgcgaaac caatgaggtg cttcaaaaag
     9541 cgatagatta ctcctccagg gccggagatc agtttgtaac gctcgaatat atcttatttg
     9601 ccctgctgca tgtaaattct caggtttcga agctgctaaa agatgccggt gctaccgaaa
     9661 aagacctgca gacagccatt aacgaactcc ggaaaggagc taaagccagc tcatcgtcag
     9721 ccgaagaaac gtataactca ctgaataaat acgccatcaa cctcaacgaa caggctcgtg
     9781 ccggaaaact cgatccggtc atcggccgcg atgaggaaat ccgccgtgta cttcagatac
     9841 tgagcagaag aaccaaaaac aacccgatcc tgattggtga accgggagtg gggaaaacag
     9901 ccattgctga aggtctggca catcgtattg tcagaggcga cgtaccggaa aatctgaaat
     9961 caaaacaagt attctcactg gatatgggag cacttgtagc aggtgctaaa tacaagggtg
    10021 aattcgaaga aagactgaaa tcggtggtga acgaggtaat ccggtctgaa ggtgaaatta
    10081 ttcttttcat cgatgaaatt cataccctgg taggtgccgg aaaaagcgaa ggagccatgg
    10141 atgctgccaa tatactgaaa cctgcgctgg cacgcggcga actgcgggct atcggtgcta
    10201 ccacgcttga cgaatatcag aaatacttcg aaaaagacaa ggcactcgaa cgccgttttc
    10261 agattgtgat ggtcaacgag cccgacattc agtcggctat ttcaatactt cgcggtctga
    10321 aagagcgcta tgaaaatcac cacaaggtac gcataaagga tgatgccatc attgctgcgg
    10381 tggaactgtc gaaccggtac atcacagacc ggtatctgcc tgataaagcc attgacctga
    10441 tggatgaatc ggcagcaaag ctccgcatgg aagtagattc ggtgcctgaa gatctggaca
    10501 agattcaaag aaacatcaaa cagctcgaaa tagaacgcga agccatcaaa cgtgaaaatg
    10561 atgtgaaaaa actcgaaaac ctgaatgctg aaattgcaaa cctgaaagaa gaaagttcaa
    10621 ttctgaaagc aaaatggtcg tcggaaaaag cactggttga gaaaattcag caaacaaaaa
    10681 ttgaaattga gaatctgaaa tacgaagccg agaaagccga acgtgaaggt gactacggca
    10741 aagtagccga gataaggtat ggaaaaataa aatctgctga agaacaaata acacaatttc
    10801 aacaggaatt agtggccatt cagggcaata acgccatgat aaagcaggaa gtggacaatg
    10861 aggacatagc agatgtggta agcaggtgga cgggcattcc ggttagcaaa atgcttcaga
    10921 gcgaaaaatc taaattgctc cacctcgaag atgaacttca taaacgggta gtaggtcagg
    10981 atgaagccat cgctgctgtt tcggatgctg taaggcgaag ccgtgcggga ttgaacgacc
    11041 ccagacggcc tattggctcc tttatttttc tcggaactac cggggttgga aaaacagagc
    11101 tggctaaagc cctggccgat tacctcttcg acgacgagaa catgatgacc cgtattgaca
    11161 tgagcgagta tcaggaaaaa ttctcggtta cacgtcttat cggatcacct ccggggtacg
    11221 taggttatga ggaaggagga caactgaccg aagccatacg ccggaagccc tattcggtag
    11281 tgcttttcga cgagatagaa aaagcacatc cggatgtatt caacgtactg ttacaggtgc
    11341 tggacgatgg ccggctcacg gacaacaaag ggcgtgtggt aaacttcaaa aacacgatta
    11401 ttgtaatgac ttccaacatc ggatcgcaaa tgatacgcga aaattttgag agaataaatg
    11461 accttaacca cgatgaaata gtagaacaga ccaaaaacga ggtaatgaac ctgctgaaaa
    11521 aagtggttcg tcctgaattt ctcaaccgga tagatgaaat catcatgttt gctccgctca
    11581 acgaaaagca aattgacgaa atagttaaaa tacaattgaa caatatttcc aacatgctgg
    11641 cagacaatgg cgtcagactg caggtaaccg aacgggcgct ggaattcatt gctcgcgaag
    11701 ggtatgatcc gcaattcgga gcccgcccgg taaaaagagc cattcaacga tacatcctga
    11761 acgatctttc gaaagagata atcagcggga aactggataa cgaaaaagta attacagtgg
    11821 attatcggga taacaagctc gtatttacaa actgatgcaa aacgcaggag ataaatggca
    11881 cctgttacat ccgctgatga tttattgagc aaaggggaaa agttgctttg gtaaaaaaac
    11941 tgttccattc aacaaacaaa acagaggctg cttctactac ggggcaccct ctgttattaa
    12001 tttttaatct ccggattcgg aaacatcgtt aaccggttta ttgaatgctg tcgggcgcta
    12061 cggtcgattg agcatcactt gcctggtctg tattacccat ttcgtcaagt tcttccagtt
    12121 cctgctgagc ttcttcggtt gactgaattt gttcctgaag aaatacctgg tcattttctg
    12181 cctcgttttt cacaaatatt acctttccca aagcaactac aaacagagct atggctgtaa
    12241 tcatcagcca ttttggcatt tttctcagag ccggttcgga ttttacaaag gccaggatac
    12301 cacctgccaa agccaaacct gtgggaagta atgctatagt tcccatcggg agaatgacaa
    12361 agatgagact aaaaaccaga aaaaaaacgg taagaataat taagaatttt ctcatacgta
    12421 aatatgtaaa ttaattgaca gttgcattgg ttaattttac ctgacccgta cccacaggta
    12481 tgctgaactg tatcaaaaca ggaattctgt tggtatcggc agtcatccag aacacgtttt
    12541 tgtcattgcc gcgcagtgct tttgtgttgg cagctataga aagtttgtaa cattcctttg
    12601 tgccgagatt tcccagattt ttgagggtct cttttcccat gaattttacc gatgcaggaa
    12661 attccttgtt gtcgaaagcg attacaaaag tgataatctg accggctctt atcttgttga
    12721 aatccatttt ccgcaaccgc agaacagccg tgacgatatc gttggtggaa gctcctacgg
    12781 gaaatgtttt agtcacctca gggctaccca ttctggtcga agtgcttgtt acttttccgt
    12841 tgctgaacac gtatttttcc gttttgcggt aatttccttc cagtgtattt cgtttgtaca
    12901 tcgaaggttt atacgtcacc ggatcaacat atgattcata caaatccctg attttgaaaa
    12961 aggtatccca cttggtataa gtagtggcaa tgaggctgct gtgaataaag gtctttttac
    13021 ttgtagttat gggttttgtc tgtatggtta tctgtgcaaa ctgagtcatc agcccgctca
    13081 tattgtaaga tccaacgtac acaaattttt cgtttttaat gttctgagcg ttggatattc
    13141 ccgtaaaaat gaaaactgcg ataattaaaa agaatttttt caccttgata aaaattattt
    13201 tttgtttatt agatagttta ttattttttt cagattgtca atgagatata ccattatcaa
    13261 tgccagcagc gagccaataa atatactgta aacaccgtat tttatccttc cgtttatcgg
    13321 caccggattc acaatcagac cggcaggaaa atctacgggc tttttatata acaccaaact
    13381 atttgtattt atattgcgaa gttcctgtaa actaagaatg tcattgtaaa acagttgttt
    13441 tctttgttct cctacaatca ggttattgtt ttcaagactg atttttttat ccgaatcctt
    13501 gaaataggaa agatttgcca ggctgtcaag tctttgtatt tcggtatcac acaaacggat
    13561 tttattttct attcctgttt ttgtactttc aaattcgctt ttcagcatcg gattgttgtt
    13621 gaaataatta agcagtgcat cctggaatat tctaatttgc gtaggtttca gggtttgaac
    13681 ttgaataaaa atgtgatttt tcataatcag attcatcgta tccttaagcg aatggctatt
    13741 atcaaaatct accatatcag gcgtattgtc atccaggtag tcgattacat tgtaactttc
    13801 gagttttaca atattctctg ccacactgtc gggtattcca agcaccgtag caaggttcat
    13861 ggagctgaat gtagttgatt ttttaggaat gagtgagttc tgaagctgac gaaaaacttc
    13921 ctttacagta gaattctcac atccgtaaag catggccatt gcattggctt cgtatcttct
    13981 tacagacgga cgagccagat actgtccggc taccacactc agcagcagca ctatagcaga
    14041 aaccacccaa tgcttgaatg ccagctgtaa taaaaaacca acaaacctga tcagccttgc
    14101 ggttactttc ttaatccatt tgcccagcag attcaataac tcgatcaagt tcatttcttt
    14161 agaatttttg tcatccatac gtattatgtt ttaagtattt caattaaatt gctgcaattc
    14221 gtcattctct gtttcatcct cttcggccat atccgataat tcgttatcgt tgtcatcatc
    14281 ttcctcccaa acttttacgg gcttttgagg gccctgcttt ctgtaagcaa acgcaagcag
    14341 cgtaccgctt actgcaccgg ccagatgacc ttcccacgac accggatcaa acttctgcca
    14401 cgggaacaaa tgccatacgt tattaccgta aagaaatacg acaataagag ctatagctat
    14461 tagcggaaca tgttttctga tgattccgct gaaaaataga aaaaaggtca gtgagtaaat
    14521 cacaccgctc aatccgatgt gccagttatc gcgtccgatt acccacagca aaagtccgct
    14581 tataataacg gataaaacca ctattttatt ggctatctcg ctgtaaaaat aatacaaagt
    14641 agtactcagc acaaaaaaag aaacaatatt attaagtaaa tgtgtcacat cggcatgtat
    14701 aaaaggcatc gaaaagatgt ttataatcgt tttcggctcg cccggaaata ctcctccccg
    14761 actgaaatcg attcccattg ccctttcaaa aacaaaaacc agaataacaa aaaaacatat
    14821 gatgagtgga aaaaacacgg cataatatat ttttttcttt tcgtgaacgc cagattccat
    14881 ataccttttt tatattttcc acaaaaataa ggataaaaat actaatacag gtcaatgcca
    14941 tcaatttgat ttcacacatt aacaaaaagt cacttacaaa ttcacgtgat agcatgaaat
    15001 ttaagacaaa tgaaaaacta aaaattaaat acgtaaacat tttgctttat ttataaaaat
    15061 tttattctta tttttgtgag tgccttaaca aaccattaat actttgtaag atgagctatt
    15121 taaattttga caaaacactc ctgatgaacc tggagaaatc tttatcaaaa gaaatgataa
    15181 ggaccaaccg cgcaggaaca tacaatagca caacactggt ggattgcaat accaggaagt
    15241 accacgggca actggtgatg ccgcttcctg acattgacaa ttcgaaccac gtgctcttgt
    15301 cgtcgctcga tgaaactgtt attcaacacg gtgccgaatt caacctgggt gtgcacaaat
    15361 actcaggaaa caattacaac cccaacggac ataaatacat ccgtcagttc gattgcgaaa
    15421 ctatatcgcg caccatttac cgcgtaggtg gagttatctt gtcaaaagag aaaatgctgg
    15481 tctcattcga gccgagaatc ctaatcaaat ataccctgct cgaagcccac tcgcctacca
    15541 ccattcgctt caaaccattc ctggcattcc gcagtgttaa tgaactgaca tatcagaaca
    15601 acgtagccaa cacatcctat aaagaggtga aaaacggcat cagtatggga ctttacgaag
    15661 gttatcccga actatacatg cagttttcga aaaaaaacac ctacttccac atgcccgact
    15721 ggtacaaagg gatagaatac acgaaagaac aggaaagagg atttgaattc aaagaagatt
    15781 tgttggtgcc cggatacttc gaaatgccaa tcaaaaaagg agattcaatc attttttcgg
    15841 gcggaatttc tgaaatatca ccaagatcac ttgacagcct gtgggaaaag gaaattttac
    15901 accgcaacca tcggttggac atgttcacca cactcaaaaa ctcagcccaa caattctata
    15961 aacgggtagg agataaaacc tacctgctcg caggatatcc gtggttcggc tcacgtgcac
    16021 gggatgaatt cgtttcacta atccccacta ccctcatcat tggacgcgaa gattattttg
    16081 acaccattgc tcagactgcc atcgaagaaa ttgaaaaatt cctcgacgga gcacgcgaag
    16141 acttcaaact ggaaggactc gaagatcccg atgttttcct gtggtttata agagctgttc
    16201 agcaatacgc cgaattccgc tcgcccgaac aggctgccga aaggtttggt gacctcgtaa
    16261 tccggatcat caattacatc aaaaaacaaa atcatccgaa ccttttcatg cacaacaaca
    16321 acctgctcta tgtgaacgga acggaaaaac cggccacatg gatgaacgct gttgaaaaca
    16381 gtcacccgat tacaccccga acgggctacg tggttgaaat taatgctctc tggtataacg
    16441 ctctgaaatt tgtatcaacg cttgaaagac tttcaggcaa agaacaatcg gccgatttgc
    16501 tggactatca ggcagaaata gcccgcgatt ctttcgtgaa agtattctgg aacggcacct
    16561 atctgtacga ctatgtggtg gatgattatc gggacatgga agttcgttcc aacatgatta
    16621 ttgccgccgg attgcccttc tcgccgctcg acaagcaaca acgcaaatcg gtggttgaca
    16681 tcaccaccaa agagctcctc acacccaaag gactccgctc gctgagcccc aaaagcggct
    16741 cttaccgccc ggaatacatc ggtggtatgc tcgaacgaag ctggaattat cacaacggac
    16801 ctgtatggcc gtggacagta ggctatttta ccgacgcata cctgcgcatt tacaagcaaa
    16861 gcggcaagtc ctttatcgaa agactgatga caggacttga agccgaaatg accgaactct
    16921 gtgtaggtac aatttcagaa ttgttcgacg gcaatccgcc tttcaaagga catggtggaa
    16981 tgtcctttgc catgagcgta gcagagatat tgagagtatt agacaagttg aaaaaaatcg
    17041 aaaacgaaat acctgcacta tgaaagcact catgttcgga tgggaatttc ccccgcatat
    17101 tctcggagga ttaggcactg caagttacgg acttacaaga ggaatgtccg aacagcctga
    17161 tatggaaatc acctttgtaa ttccaaaacc tcacggcgac gaagatcaga gtttcctgaa
    17221 aattatcggg gcatgcaatg tgccggtggt atggaaagaa aacagttggg aacatgtaaa
    17281 taaccgtatc ggtaaaatca tgcatcccga cgaatacttc tggcttcgca acggcattaa
    17341 gtacgacttc cgaaggattt ataccaacga cctgggttgt atcggatttt cgggaaaata
    17401 ccccgacaac ctcattgaag aaatatccaa ctatgaagca gtagctagtg tgctggctca
    17461 tcagcttgat ttcgatgtaa tccattcaca cgactggctc acctatcctg ccggacgctt
    17521 tgcaaagcaa ataagcggaa aaccgctggt aattcacgta catgccaccg actttgaccg
    17581 cagccgcggg aaagtgaatc ctaccgttta caaaatcgaa aaagacggaa tggatgctgc
    17641 cgaccacatc atgacagtaa gcgacctgac gcgccgtacg gtaatagaga aataccacca
    17701 gcacccaaac aaggtaacca ccgttcacaa tgcagtagaa ccacttccgg atgtggactc
    17761 atttgttaag actccgcgaa aagacaaaat agtgactttc ctcggccgaa taaccatgca
    17821 gaaaggcccg gagtattttg tcgaagcagc agcacgcgta ctgagcaaaa caaaaaacgt
    17881 gagattcgtg atggccggca gcggcgatat gatgaatgcc atgattgacc tggctgccaa
    17941 acggggtatt gccgatcgtt ttcatttccc cggattcctg cgtggaaggg atgtgaatga
    18001 gatgctggct cagagtgatg tctacatcat gccttccgtt tccgaacctt ttggcatttc
    18061 gccactcgaa gccatgcagg taggtacacc atccattatt tcataccagt cgggttgtgc
    18121 cgagatcctc acgcatgtgg tgaaaaccga ttactgggat attgacgcca tggctgatgc
    18181 catctacggt atagtgactt atccggccat gtataaaagc atgaaagaac tgggtctgga
    18241 agaggtgaac aacatcaaat ggtacgatgc cggattgaaa gtacgggcca tttacgataa
    18301 agtgattcga aaacactgaa atatctgaag aaaaaaaact caatgcaagt ttgaactgac
    18361 ttcacaaaaa aaatccaatt aaatattaat tccgaaatta aagctattaa gatatgaaaa
    18421 acatttgttt ttattttgag attcaccagc cgctgcgtct gagacggtat cgtttttttg
    18481 aaattggtca ggaccattac tattttgacg atttccagac cgaagaccgg atacgcggta
    18541 tggttgagca ggcttatctg cctgctatcc gcaccatcgc agagatgata cgcagttcga
    18601 acggaaaatt caaatgctcg ttctccattt cgggtgttgc gatggagcaa ctggagcaat
    18661 atgctcccga agtaatcgac ggcttcaggg atctggccaa aacaggcagt gtggaatttc
    18721 tggcagagcc ttacgctcat tcgctggctt cggtttacaa ccggagtgaa ttcgaacagc
    18781 aggtaaaact tcactcaaaa aaaatagaag ccttgtttga gaaaaaaccc acagtgtttc
    18841 gaaactccga actgatttat tccgatgaaa taggtgaaat tgtgtcggcg ctcggattca
    18901 aaaccatgct gatagaagaa gtgaaatatg tgatgggatg gaaaagcccg aactttgttt
    18961 acaaccactc atacatcccg aaactgaaac tgctggtcag aaacaacaaa ctgagcgacg
    19021 acattgcatt ccgtttctct gatcattcct ggtcagattt tcccctttcg gctgaaaaat
    19081 ttattcgctg gatcagtgag ttgcccaagt ccgatcccgt agtgaatctg tggatgggat
    19141 tcgaggcatt cggattctat cagcgcgctg aaagcggtat tttcgaattc ctgaaagccc
    19201 tgccttacca cgcaatggaa cagggtcaca gttttgcatt accatctgaa ataaccaaaa
    19261 aatttgattc ggtcgacagc ctgacatctc cttatccaac cagctggtcg ggcgaaaaag
    19321 acctgagcgt atggaacgga aacgaccttc agcaagaggc tctcaacaaa ctctatgctg
    19381 taagtgaacg ggtacatctc tgtaaggatg ctccgctgaa aaacgactgg cttaaactgc
    19441 aaactacgga taacttccgc tacatgtcgc acttcgatgc ctttggtacg cattacactt
    19501 cgccttacga agcttttacc aattacatga acgtgctggc cgattttctc gacagggtgg
    19561 atgcccaata ccctacaacc attgagaacg aggaacttaa cgaactgtta aaaaccatta
    19621 tcagtcagga gagcgagata gcaacacttg aagaagagct gaaaaaagca cgggcaagaa
    19681 aaaagaaaga ggaataattt caaatccgga tttggtttgc cctgtttgag gtcagtcatt
    19741 cggatgaaat ccagattgca gtgacaaaaa aaagccgaag agagttttac ccgcgggtga
    19801 agctctcttc ggctttatcg ttatgatggt ctatcactgc tttcgcatat caaatccgag
    19861 cagcatacca tcatacatat cggccatttt gcccagcacc tcgatagaag catcattggt
    19921 aaactgactg ataattttca taagtttcag gaatgaatcg gcatcaaaga gtagcttcat
    19981 ttcgcgcgag gatacggcta cagtagcttt cagcttgccc acaccgagca gataagtcat
    20041 ttgtacctgt tttgattcgg gattaagcat gtatttacct gaaatcttca tgccgccgat
    20101 gcgtccggtg taggtactgt cggcatggaa gacaaaacct gtattctttt cgctgatgcc
    20161 aagtttggtg cacgtttcgt caaatttatc ctccacctgg gtggctacca ctgccccacc
    20221 ggctttttta agcagatttt cggtttcgaa agcgcacgaa gttccctgaa agttccaggt
    20281 cccgaccatg ctttgctgcg taatcgggag ttttcccata acagtattga atatatcttc
    20341 gagcacattg cccgagccgg tctttttatt ctccgacttc gcatccgatg tacccgaaag
    20401 cagaccgcag gaactgaata caactgctga actcactacg agcaggaaaa acagatttct
    20461 aaatttcatt tacattaatt attttaattc gtcgtacaaa agtagttttt tgttttttat
    20521 aaatcaaaaa tcccgccaca aattgcagca ggattctgtg taagaaatcg taattacttt
    20581 tgcgagatca ctgtatgtta ctcaaaatca aatcactttc aacaaccgga gtataaaaca
    20641 cacaggataa agcttactga agcttccgtt tctgacgaaa ttttcacccg acgtatttta
    20701 ttaaatattg aactttcgca agtatcataa atgcttgaaa tactgattaa ttgaactttc
    20761 gcatgttcat attagtttat aaatcataag attaagaatg aataatatct gatagtcaga
    20821 tagatataca ttttttcagg aacaggggct tccgcccctt agaccctgac ttactttttg
    20881 tcttgaaaca aaaagtaagc aaaaaatt
//
LOCUS       DAORKP010000020        20079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_122_length_20079_cov_7.476328, whole genome shotgun sequence.
ACCESSION   DAORKP010000020 DAORKP010000000
VERSION     DAORKP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_20079_cov_7.476328"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..450)
                     /locus_tag="PLN75_02250"
     CDS             complement(229..450)
                     /locus_tag="PLN75_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02969.1"
                     /translation="MNFYKLLSRLRQGAFYGNRTNKLNRPWFMSSNETKEGEHNHTVV
                     HEMKRSLIRNGSRGEGIGMGHRNKKSAAK"
     gene            complement(621..1685)
                     /locus_tag="PLN75_02255"
     CDS             complement(621..1685)
                     /locus_tag="PLN75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG02970.1"
                     /translation="MNNIIRTGIIGFGLSGRVFHAPFIDVVDGFELTKISTTNPQNIE
                     MIRKRYPATEIVAGAEEIINDSQIDLVIVTSPNTVHFKWAKEALLAGKHVVIEKPFTN
                     TTQEADELIELSKKQRRILTAYHNRRFVSDTKTVKKIISSGLLGDVVDYETHFDRYRP
                     EPRPEGYWRERPLPGSGIFYDLGSHLIDQALHFFGLPLAVTAHIASQRPWAEADDSFD
                     VKLHYPSFTATLKSSMLAKIPGPTFQIHGTKGSFVKYGLDVQEENLNRGMIPDTPDWG
                     KEPEKIWGKLVTEFQGVKMESIVESENGDYREYFYNLRDTILGKARLAVTPEEARNVI
                     RIIELAFQSNLEKRTIDVVL"
     gene            complement(1786..2901)
                     /gene="recF"
                     /locus_tag="PLN75_02260"
     CDS             complement(1786..2901)
                     /gene="recF"
                     /locus_tag="PLN75_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810122.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRG02971.1"
                     /translation="MRLNSLSILNYKNIVQADLNFPAKINCFVGNNGMGKTNLLDAIY
                     YLSFCKSHSNSIDTQNINHEADFLMLQGKYELRGNEEEVYCGIKRRQKKHFKRNKKEY
                     SRLADHIGLIPLVLISPADYSLIAEGSDERRKFTDGVISQYNNQYLNALVHYNHALQQ
                     RNALLKDENATDNSLFEIWEEQMGMHGKYIHAERKQFIERFIPLFQGIYSFISEDNER
                     VGLEYISQLDEADLTQKLASNRERDRIIGFSMNGIHKDELEMTLAGHPMKRVGSQGQN
                     KTYLVSLKLAQFNFLKQTHGVTPILLLDDIFDKLDEKRVTKIVELVSDNDFGQIFISD
                     TNSRHWEEILPKINQTSMIFNVVNGEISEAKSIQSDE"
     gene            2985..3617
                     /locus_tag="PLN75_02265"
     CDS             2985..3617
                     /locus_tag="PLN75_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02972.1"
                     /translation="MKAKKLHFILYILFFQLIFVACNTGNSKHDDFDYGTVDKGIYNN
                     SYFRFSVKVPADWNTLTQEQNQNLMNKNTLGSNDPSKITTVILLTASQFEPGKTDSTF
                     NSNMIILAENIKGNTRIRNGIDYLRLTRQALEKEPILRQYPDSNAPVMKIKGSDFSTM
                     EVTTTDGNKTYSQKYYTILVNDFALTAILTYATEDERIILENAFSTINFK"
     gene            3624..5747
                     /locus_tag="PLN75_02270"
     CDS             3624..5747
                     /locus_tag="PLN75_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRG02973.1"
                     /translation="MLTEHYIQLLSTSLQLTRKQILNTIALLEDGATIPFISRYRKEM
                     TNGMDEVQLAELKEQYDKLIELIKRKETILKSITEQEKLTPDLQKRIEECWNATELED
                     IYLPYKPKKRTRAEIAREKGLEPLAKILMAQHSNQVFTSAKPFITSEVTSEEEALKGA
                     RDIIAEWVNESEAARNAVRSNFSHEGVITSKLIKGKEDEAAKYRDYFDASEKLSRISS
                     HRLLAMRRGEAEGFLRVGISGDDDKCMERLLRIFRKSDNESGNQVAEAVEDSFKRLLK
                     PSIENEFATSSKEKADTEAIRVFTENLRQLLLAPPLGQKRVMGIDPGFRSGCKVVCLD
                     AQGNLLHNENIYPHPPVSEHSQAVRKIQKMVESYDIQAIAIGNGTAGRETEQFIQNIR
                     YDRKVQVFSVSENGASVYSASKIARDEFPEYDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQVKLKKSLDQTVENAVNRVGVNLNTASKHLLTYISGLGPQLAQNIV
                     DFRAENGAFANRAELKKVPKLGAKTFEQCAGFLRIPHGNNPLDNSAVHPESYSIVVKM
                     AKDLNRDIDDLIGKKDIRSSLDLSKYVTAKVGLPTLNDILDELEKPGRDPRSVIKVFE
                     FDPNVKNIKDLKEGTELPGIITNITNFGAFCDVGIKENGLIHISNLSDSFVSNPADVV
                     SLHQHVQVRVLEVDFERKRIQLKLI"
     gene            5777..6496
                     /locus_tag="PLN75_02275"
     CDS             5777..6496
                     /locus_tag="PLN75_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG02974.1"
                     /translation="MRNLMIYLPVFLFFIAGFQLKAEENTGAKIENPLMIENELVVQD
                     PALSSGLVWDKNKPKAKAKQKAPDFDKQLWKNFHIGWDIDKSEDYRWRNFAIGTGYTR
                     NFPTVPRAYWYVGGDVNWSKYTLYHGRQFNTTGNDYRLNTFSLSVPAYLGYNLYKSSL
                     RAFGVKVYTGPTIELITSMKRDGYAYKEYNPFQIGWTVGTGVKLFYMFGFNVAYHYYP
                     IPVLTDGNLVRSSVNFTWFLN"
     gene            6661..7536
                     /locus_tag="PLN75_02280"
     CDS             6661..7536
                     /locus_tag="PLN75_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRG02975.1"
                     /translation="MIQSMTGYGKAFCEINNKKIVVEVKSLNSKQIDLSTRISGIYRE
                     KEIEIRNLLINKLERGKIDFSLYIDSSGKESGAKVNQAVVKSYYEQLQELSKQTGIPQ
                     TQDWLAILMRLPETLKTEVEELDENEWNEILKTINEAVNQLIEFRNQEGNALESVFMQ
                     KIEKIAKLLPEIEPYETERIEKIKNRLESNLEGISDKIEYDKNRLEQELIFYIEKLDV
                     NEEKVRLKNHLDYFISTMQTEKSPGKKLGFIAQEIGREINTLGSKSNHSEMQKIVVEM
                     KDELEQIKEQVLNTL"
     gene            7651..8322
                     /gene="gmk"
                     /locus_tag="PLN75_02285"
     CDS             7651..8322
                     /gene="gmk"
                     /locus_tag="PLN75_02285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRG02976.1"
                     /translation="MTDSINFNNNDNHKVLPKETCLQPRQVGINQARSGGRVIIFSAP
                     SGSGKSTIVNHLLRCDLGLEFSISATSRPPRGNEANGIEYFFFSKEEFEKKIEKDEFV
                     EYEEVYPGCYYGTLRSELERISAKGAVVIFDVDVMGGINLKNKFGDDALAVFIAPPSI
                     EILRDRLINRCTDSDEMIEKRVGKAKYEMGFAQEFDRIIVNDNLEKAKKEAEKVVREF
                     LKEAP"
     gene            8738..9322
                     /gene="nadD"
                     /locus_tag="PLN75_02290"
     CDS             8738..9322
                     /gene="nadD"
                     /locus_tag="PLN75_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRG02977.1"
                     /translation="MKSIGLFFGSFNPVHLGHTSLAEYIFEFSGVDEIWFVVSPRNPL
                     KEQSELIDEHLRLRMLHLATENTEYLVPSDIEFELPKPSYTINTLKTLSERFPEDNFI
                     LLIGSDNMQIFDQWKDYQTILDDYSVLVYPREGYSYEEFEEKYPEMQILEEAPFFDVS
                     STEIRTLIKNKQDASPWLHPEVYQFIRDNGLYTD"
     gene            complement(9358..9993)
                     /locus_tag="PLN75_02295"
     CDS             complement(9358..9993)
                     /locus_tag="PLN75_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRG02978.1"
                     /translation="MNVKVFPFNPLQENTYLIWDETGEAAVIDAGMLFDKEKERIKRF
                     IEENQLTLKRVINTHLHFDHQFGNKFLHETYGIMPDAGAEDEFLLDSAHLVAKNWGIP
                     YSESVQRPGSYITDNQIINFGNTSLIAMHTPGHSPGSFSLYCKEAGVVFVGDVLFNSS
                     IGRTDFVKGDYATLIKSIEERLMTLPDETVVYCGHGPSTTIGYERQYNPFL"
     gene            complement(10143..10763)
                     /gene="rsmG"
                     /locus_tag="PLN75_02300"
     CDS             complement(10143..10763)
                     /gene="rsmG"
                     /locus_tag="PLN75_02300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRG02979.1"
                     /translation="MDLILKYFTNLNETQVSQFGSLYDLYVDWNSKINVISRKDIDNL
                     YERHVLHSLGIAQVIHFKAGTRVLDVGTGGGFPGIPLAIFFPEVHFTLIDSIGKKIKV
                     TQEISSFIQLKNIELKHSRIQEEKNKYDFVVSRAVMPLAELVKLCRKNISPKQQNALP
                     NGLICLKGGELEHEILPFRNTAETYALKDFFSGEFFETKKIVYLPL"
     gene            10931..11806
                     /gene="fabD"
                     /locus_tag="PLN75_02305"
     CDS             10931..11806
                     /gene="fabD"
                     /locus_tag="PLN75_02305"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRG02980.1"
                     /translation="MRAYIFPGQGAQFTGMGKDLYENSPLAREYFEKANEILGYRITD
                     LMFEGTSEDLRQTKVTQPAVFLHSVISAIALGDKFKPDMVAGHSLGEFSALVAAGALT
                     FEEGLRLVYARAMAMQKACEKEPSTMAAVLGLDDETVEEVCDEVNKEYIVVAANYNSP
                     GQLVISGSIEGIDKACELLKQRGAKRALKLPVGGAFHSPLMEPARQELQAAIEKAVVS
                     TPVCPVYQNVNANPQTDPESIKKNLIAQLTAPVRWTQTVKNMINDGADEFIELGPGDV
                     LKGLVKKVNPDIITG"
     gene            11977..13875
                     /locus_tag="PLN75_02310"
     CDS             11977..13875
                     /locus_tag="PLN75_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRG02981.1"
                     /translation="MSKKLLIRDLTLRDGQQSSFATRMSQPQIDRVLPYYKEANFYAM
                     EVWGGAVPDSVMRYLGENPWHRLEKIHKGVEGVSKLTALSRGRNLYGYAPYPDEIIDG
                     FFKNAVSSGLDIMRIFDALNDVENIKSSVKSIKKYGGMADCAVCYTIDPKYDDEIKIV
                     EKKGFLGLFNKKEEVVVKRQPVFTDTYFLNKAKEMEALGADMITIKDMSGLIPPSRVA
                     SLMTLFKKELKVPVDFHTHCTPGFGLGSVLTAIMYGVDVVDTNIWYFSGGPAAPAIEL
                     IYLFCKKLGIELDVNMKAVAKINKELEGIRRELAAFDTGKHFPKAFNPLTDKLPAEID
                     KEFDKAIEAVKKEDENALLAACYAIEAYFEFPKPDDLVRKAEVPGGMYTNMVAQLKQF
                     NSMDILEDALHLIPSVRLDSGLPPLVTPTSQIVGAQAVASALNIKNGRPKYANPSNQF
                     IALVKGEYGKTPVPVDPKFREQITGSPREMPYDTSGYKRQDNPVLPEFGNVKLAKDEK
                     DELLLELFPSVATNYLRGIRENEYKLNPQPAQVAETKETVKKETVTEVQQPVGKKMKC
                     PLPGTILSVKVKVGDVVKDGQTLFILEAMKMENNITSEFNGKVINIFVKPGEIVPEGA
                     PLVEIAHE"
     gene            14066..15820
                     /gene="aspS"
                     /locus_tag="PLN75_02315"
     CDS             14066..15820
                     /gene="aspS"
                     /locus_tag="PLN75_02315"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRG02982.1"
                     /translation="MFRNKTCGELRLADAGKNVTLAGWVQRTRKMGGMTFVDLRDRYG
                     ITQLVFNQDVNAILCEQANKMGREYVIQITGTVAERSNKNSNIETGEIEILVDTLRIL
                     NESKTPPFTIEDNTDGGDDIRMKYRYLDLRRNSVRANLELRHKMTIAVRQYLDSLGFL
                     EVETPVLIGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQDDVLNTFEGLTKHLFRSLKGLEISEPFLRMTWA
                     DAMKYYGSDKPDMRFEMKFTELKEIVSGHNFSVFDDAEYVAGICAEGCAGYTRKQIDE
                     LTDFVKRPQIGAKGLVYIRYNEDGTLKSSIDKFFTEDSLAGIASQFNAQKGDLILILA
                     GDKRKTQKALCELRLEMGERLGLRSKDKFAPLWVVDFPLFEWDEEEQRYFAMHHPFTS
                     PKPEDIHLLDTNPGEVRANAYDMVINGVELGGGSIRIHDSQLQSKMFELLGFTPEKAQ
                     AQFGFLMGAFQYGAPPHGGLAYGLDRFVSMFAGLDSIRDCIAFPKNNSGRDVMLDAPT
                     EIDQSQLDELNLIVNLKK"
     gene            complement(16464..17390)
                     /locus_tag="PLN75_02320"
     CDS             complement(16464..17390)
                     /locus_tag="PLN75_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRG02983.1"
                     /translation="MTLQQLEYIVALSKTKHFVQAAEMSGVTQPTLSMMIQKLEDELD
                     VKIFDRSKHPIGITAIGNKIVDQAQVVLCQLNKINEMVESEKGDLTGHINLAIIPTVA
                     PYLLPKFFAEFKRNYPQLSFKMYEMHTDTIERKLMLGDIDMAILSTPLSNPNVLEIPL
                     YYEKFIAYISPNNELYKHTELISGDLPLEHLWVLEEGHCLRNQVFNFCNSGIVTSYAY
                     ESGSIDTLVKIVDENGGYTVIPELHVELLTDIQKKNLRKLVEPEPAREISLIIQQNFI
                     REGQLNAVAETVKKIIPPHMLDSRLKKFAIKI"
     gene            17552..18424
                     /locus_tag="PLN75_02325"
     CDS             17552..18424
                     /locus_tag="PLN75_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRG02984.1"
                     /translation="MNRIKLLILLVIICISTVNAQLLWKISGHGLKQNSYLLGTHHLI
                     SISFLDSIPGVYPAFNKSKMVVSEIVFSNQDVSAKIQKASTLPDSVTMKNLLSAEDFD
                     FVNKELIDVLKMNLLSINKMHPSLIQTFYELELYKERAHFDPNTKSDSYFQLVASQKG
                     IPVAGLETVDKQIELLFPKDNLKKSAEELVKTIRKKDLLFADYQQLNKLYRSGDIEGL
                     SEMNKAWNREWGVTAEENARQLDLRNIEWVKQLPELMKSNSCFIAVGALHLPGENGLI
                     HLLRKKGYKVKPVK"
     gene            18539..19903
                     /locus_tag="PLN75_02330"
     CDS             18539..19903
                     /locus_tag="PLN75_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRG02985.1"
                     /translation="MSKMKKFLLSENEIPTQWYNITAEMPNKPLPLINPGTKEALKPE
                     DLFPIFSQEVSRQEMNNTDVWIDIPDEVREKYMIYRPTPLVRAYNLEKALDTPAHIYF
                     KNESVSPVGSHKLNSALPQAYYCKKEGITNVTTETGAGQWGTALAFATKAFGLELAVY
                     MVKISYEQKPYRRSLMQTWGAQVIASPSMSTKAGRKIITEHPNYQGSLGTAISEAIEL
                     ALSTPNCRYTLGSVLNHVMLHQTVIGLEAEKQMEMAGEYPDVVIGCFGGGSNFSGISF
                     PFMRHNIKNNAKTRFIAAEPASCPKLTRGVFQYDFGDEAGYTPLIPMFTLGHNFAPAH
                     IHAGGLRYHGAGSVVSQLMKDGYMEAMDIPQLESFEAGVLFAQTEGIVPAPESAHAIA
                     SAIREAKKAKEEGVKKTILFNLSGHGFVDMSAYDQFLAGDLRNYELTADDIAKTTEIL
                     ERIV"
BASE COUNT         6289 a         3974 c         4140 g         5676 t
ORIGIN      
        1 atttgagaaa aaacctggcg tgaaggacga acaaaagctt aaatcgttta tacaggaaat
       61 tcgcaggaaa gaagattatg gccttatacc ttccaggctg gtatgactta aaaaaaacta
      121 ctcctgctat ctgtttgatg aaagtcaggt aaaaatacac aacattccct catgtctgat
      181 tattctgaca atgaatcact tatcccatcg ttaagttttc gcttattttt atttagctgc
      241 tgatttctta tttctgtgtc ccattccgat accctcgcct cggctgccat ttcggattaa
      301 acttcgcttc atctcgtgaa ctaccgtatg attgtgttct ccttctttcg tttcattact
      361 gctcataaac cacggcctgt ttagtttatt tgttctattg ccgtaaaaag ctccttgtct
      421 caaccggctt aacaatttgt aaaaattcat agtctcaaaa tattaagtta aacgaatgag
      481 aaaataacta tccaataaat aaacaaatac ctgatttaat tagttgaatg gataattatt
      541 aatctgtatt aaattataat ttttacctca gattactcaa ataatccttt tcattcgatt
      601 attaaaagat attggttttt ttacaaaact acatcaatgg tccttttttc cagattgctt
      661 tgaaatgcaa gctctattat acgaattaca tttcgggctt cttcgggtgt cactgccagc
      721 ctggctttac ccaatatggt atctctcaaa ttgtaaaaat actcgcggta atcaccgttt
      781 tcgctttcta cgattgattc catttttact ccctgaaatt ctgtaaccag tttcccccaa
      841 attttttcag gttctttacc ccagtcgggg gtgtcgggga tcataccccg gttcaggttt
      901 tcttcctgta cgtccagtcc gtatttcaca aatgaacctt tggtgccgtg gatttgaaaa
      961 gtcgggccgg gaatttttgc cagcatcgat gatttcagcg tagctgtaaa agaagggtag
     1021 tgtagtttca cgtcaaagct atcgtctgcc tctgcccacg gacgctgaga agctatgtga
     1081 gccgttacgg caagcggcaa tccgaagaaa tgcagcgcct ggtcaatcag atgcgaaccg
     1141 aggtcgtaga aaattccgga gccgggcagt ggtctctcgc gccagtatcc ttccggacgt
     1201 ggttcaggtc tgtaacggtc gaaatgagtt tcgtaatcca ccacatctcc gagcaaacca
     1261 ctcgaaatga tttttttcac ggtttttgta tcgcttacaa atcgccggtt gtggtatgct
     1321 gtaagtattc tacgctgttt tttgctcagt tcaatcaatt cgtcggcctc ttgagtggtg
     1381 ttggtgaatg gtttttcgat aaccacatgt ttgcctgcca gcagtgcttc ttttgcccat
     1441 ttgaaatgaa cggtattcgg tgatgtcaca attaccagat cgatttggct gtcatttata
     1501 atttcctcag ctcctgcaac aatttcggtg gccggatacc gtttcctgat catttcgatg
     1561 ttctgaggat ttgtagtgct gatttttgtc aactcaaatc catccaccac atcaataaag
     1621 ggtgcatgaa aaactcttcc ggacaatccg aaaccaataa tgcctgttct gatgatattg
     1681 ttcattttaa taagttgatt attaatgtgt tgttatttat aaattattag tgtaaatttt
     1741 tatatcaacc tgtggagatg acattattca tcataaacaa aatcattatt catcggattg
     1801 aatacttttt gcttcagaaa tttctccatt cactacgttg aaaatcatcg aagtttggtt
     1861 gattttagga agtatttcct cccaatgccg gctattggta tccgaaataa aaatctgtcc
     1921 gaaatcatta tcagatacca gttccactat tttcgtcacc cgtttttcgt cgagtttatc
     1981 gaaaatatca tcgagcagca gaatgggtgt tacaccgtga gtttgtttca gaaaattgaa
     2041 ctgagccagc ttcagtgaca ccaggtaagt cttgttttgc ccctgcgagc ccacacgttt
     2101 catcggatgt ccggcgagcg tcatttccag ctcgtctttg tgtatcccgt tcatactgaa
     2161 gcctataatt cggtcacgtt ctctgtttga agcaagtttc tgagtcaaat cagcttcatc
     2221 cagttgcgat atgtactcca gtccgacccg ttcattgtct tccgaaataa agctgtagat
     2281 accctgaaac agcggtatga atcgttcgat aaactgtttt cgttcagcgt gaatgtattt
     2341 tccgtgcatt cccatttgct cttcccatat ctcaaaaagt gaattgtcgg ttgcgttttc
     2401 atctttaagc agggcgttac gctgttgcag ggcgtggttg taatgaacca aagcattgag
     2461 gtactgattg ttatattgtg agattacacc atccgtaaat ttacgccgtt cgtcacttcc
     2521 ttcagctatg aggctgtagt cggctggcga aataagtacc aatggaatca gaccgatgtg
     2581 atcggccagc ctgctgtatt cctttttgtt ccgcttgaaa tgctttttct gccggcgttt
     2641 tattccacaa taaacctcct cctcgttgcc ccttaattca tatttaccct gaagcatcag
     2701 aaagtcggct tcgtgattaa tattctgtgt gtcaatagaa tttgagtgac ttttgcagaa
     2761 cgacagataa taaatcgcat ccagcagatt ggttttcccc ataccgttat tacccacgaa
     2821 acagttgatt tttgccggaa aattcagatc ggcctgaacg atgtttttat aattgagtat
     2881 agatagtgaa tttaatcgca tttatatttt gttagggttc aaaaatacaa aaataggaaa
     2941 aatatcctta attttgtaat tcaatgtaga taaactcaaa cattatgaaa gcaaaaaagc
     3001 tgcattttat cctgtatatc cttttttttc aattaatttt tgtagcatgc aatacaggta
     3061 acagcaagca tgatgatttt gattacggca cggtagataa aggtatttat aacaattcct
     3121 atttccgctt cagcgtaaaa gtgcctgctg actggaatac acttactcag gagcaaaatc
     3181 agaatctgat gaacaaaaac acgttgggca gcaatgatcc ttcgaaaatt actactgtca
     3241 ttctgctaac tgcatcgcag tttgaacccg gtaaaacgga ttcgacattc aattcgaata
     3301 tgattattct ggctgagaat attaaaggaa acacccgcat cagaaatggg attgactacc
     3361 tgagactcac tcgccaggca cttgaaaaag aacccatcct aaggcaatat ccggatagca
     3421 atgcgccggt aatgaaaata aaaggatctg atttttcaac tatggaagtc actacaacag
     3481 atggaaacaa aacgtattct caaaaatact atacaatact tgttaacgat tttgcactca
     3541 cagccattct cacttacgcc actgaagatg agcgaattat tctcgaaaat gccttcagta
     3601 ccattaattt taaataaacg ttcatgttaa ccgaacatta cattcaactc ctttcaacct
     3661 cgctacaact taccagaaaa cagattctga acacgatagc tcttctggaa gacggagcta
     3721 ccattccgtt catcagccgc taccgcaagg aaatgacaaa cggcatggat gaagtgcagc
     3781 ttgcagagct caaagaacaa tatgacaaac tcatagaact gattaaacgg aaggaaacga
     3841 ttctaaaatc cataaccgaa caggaaaaac ttactccgga ccttcagaaa cgcattgaag
     3901 agtgctggaa tgctaccgag ctggaggata tctatcttcc ttacaagccg aaaaaacgta
     3961 cacgcgccga aattgcacgc gaaaaaggat tggagccgct ggcaaaaatc ctgatggctc
     4021 aacattcaaa tcaagttttc acctcggcta agccatttat aaccagcgaa gtaaccagcg
     4081 aagaggaggc gcttaaaggc gcccgcgaca ttattgccga atgggtcaat gaaagtgaag
     4141 ctgcccgcaa tgccgtacgc tccaattttt cgcacgaagg agtgataaca tcaaaactga
     4201 ttaaaggcaa ggaagatgaa gccgcaaaat accgcgatta ttttgatgca tccgagaaat
     4261 tatccagaat cagttctcac cgtttgctgg caatgcgaag gggtgaagcg gaaggatttc
     4321 tgcgagttgg aatatccggt gatgatgata agtgcatgga gcgattatta agaatattca
     4381 ggaaatcgga caatgaaagt ggcaatcagg tggccgaagc agtggaggat agcttcaaac
     4441 gattgttgaa accatccatt gaaaacgaat ttgcaacttc gagcaaagaa aaagccgata
     4501 cagaagccat tcgtgttttt actgaaaatt tacgccagtt actccttgca ccacctctcg
     4561 gtcaaaaaag ggtgatgggt atagaccccg gatttcgtag cgggtgcaaa gtagtatgcc
     4621 tggatgcgca gggaaacctg ctccacaatg agaatatcta tcctcaccct cccgtcagtg
     4681 agcattcgca ggcagtacgc aaaatccaaa aaatggtgga atcctatgat attcaggcta
     4741 ttgctatagg taatggaaca gcaggccgag aaaccgagca attcattcaa aacattcgtt
     4801 acgacaggaa agtgcaggtg ttttcagtaa gtgaaaacgg cgcttccgtc tattcggctt
     4861 ccaaaattgc gcgcgatgaa tttcctgaat acgacgttac ggtgcgcgga gcagtttcta
     4921 tcggtcggcg actgatggac ccactggcag aactggtaaa aattgacccg aaatctatcg
     4981 gagtaggaca atatcagcac gacgtggatc aggtgaaatt aaaaaaatcg ctcgatcaaa
     5041 ctgttgaaaa tgcggtaaac agggtaggag tgaacctgaa tactgcaagc aaacatctgc
     5101 tcacatacat ttcgggtctg ggacctcagc ttgcacaaaa tatcgtagat ttcagggccg
     5161 aaaacggagc ttttgccaac agggctgaac tgaagaaagt gcccaaactc ggtgcaaaaa
     5221 ctttcgaaca atgtgccggc tttctgcgca taccccacgg caataatccg ctcgataact
     5281 cggctgtaca ccccgagagt tattccatag tagtaaaaat ggctaaagac ctgaatcggg
     5341 atatagatga tctgataggg aaaaaagata ttcgaagttc gctcgatttg tcaaaatatg
     5401 taacagctaa agtaggattg cccaccctga acgatattct ggatgaactt gaaaagcccg
     5461 gaagagaccc aagatcggtt atcaaggtat ttgaattcga tccgaatgtg aaaaatatta
     5521 aagatttgaa agaaggcacg gaattacccg gaataattac caatattaca aactttggcg
     5581 cgttttgtga tgtcggaatc aaagaaaacg gattgattca catttccaac ctttccgaca
     5641 gttttgtgag caatccggcc gatgtggttt ccctgcatca gcatgtgcag gtgcgggtgc
     5701 tggaagtgga ttttgaacga aaacggattc agttaaaatt aatataatcg aaaaataatt
     5761 attaaaacag ttgactatga gaaatttaat gatttacttg cctgttttct tgtttttcat
     5821 cgcaggtttt caactgaaag ctgaagaaaa tacaggagcc aaaattgaaa atcctttaat
     5881 gattgaaaat gaattagttg tgcaagatcc agccctgagt tccggattgg tttgggataa
     5941 aaataaaccc aaagcaaaag cgaaacaaaa agcaccggat tttgataaac aactgtggaa
     6001 aaattttcat atcggatggg acatcgacaa atcggaagat taccggtggc gaaacttcgc
     6061 tatcggcaca ggttataccc gtaactttcc aaccgtaccg agagcttact ggtacgtagg
     6121 tggcgacgtg aactggagca aatatacgct ttaccatggg cgtcaattta acactaccgg
     6181 aaacgattac cgactgaata ccttttcgtt gtctgttccg gcatacctgg gctacaatct
     6241 gtacaaatct tcactcagag cattcggggt gaaagtatat acaggtccta ccattgaact
     6301 gataacttcc atgaaacgcg acgggtatgc ctacaaagag tataatccgt ttcagatagg
     6361 atggacagtt ggtacaggag tcaaattgtt ttacatgttc ggtttcaatg tagcttatca
     6421 ttactatccc attcctgtgc tgaccgatgg aaacctggta cgctcgtcgg taaatttcac
     6481 ttggttttta aattaaatct cagtatataa ataaggaatc atctgaatga cagttgattc
     6541 cttttttatt tacataaata atgtatcgca acaagcatac attctcacta aaatgtgaat
     6601 gaaaaggaca tctcaactga ttttttatag tatttttgtg gaataaaaca taaaattagt
     6661 atgatacaat ccatgaccgg ctacggaaaa gccttttgcg aaatcaataa caagaaaatc
     6721 gtagtcgaag taaaatcgct caacagtaag cagatagatc tttcgacccg gatttccggt
     6781 atttatcgtg aaaaagaaat tgaaatacgt aatttgctca ttaacaagtt agaacgggga
     6841 aaaattgatt tttcacttta tattgattct tccggcaaag aaagcggtgc gaaagtaaat
     6901 caggcagttg taaaatcgta ttacgagcag cttcaggaac tatcgaaaca aacaggaatt
     6961 cctcaaactc aggattggct ggcaatattg atgcgcctgc ccgaaaccct gaaaaccgaa
     7021 gtggaagagc tggatgaaaa cgaatggaat gagattctga aaacaatcaa cgaagctgtc
     7081 aatcagctga ttgaattccg taaccaggaa gggaatgctc ttgaatctgt atttatgcag
     7141 aaaattgaaa aaatagcgaa attgcttccg gaaattgagc cgtatgaaac cgaacggatt
     7201 gaaaagataa aaaatcgtct ggaaagtaat ctggaaggca taagcgacaa aatagaatac
     7261 gataaaaacc gcctggaaca ggagttgatt ttttacatcg aaaaactgga tgtgaacgaa
     7321 gaaaaagttc gcctgaaaaa tcatctcgat tacttcatca gcactatgca aaccgaaaaa
     7381 tctcccggta aaaaacttgg ttttattgcg caggaaatag gcagagaaat aaatacgctc
     7441 ggatcaaaat ccaatcacag cgaaatgcaa aaaattgtgg tggagatgaa agacgaactg
     7501 gagcaaataa aagaacaggt tttgaataca ttgtaaaagc cccctgaacc cctcccgaaa
     7561 caagtacggg acaggctcga agggagactt ttcattcagg ataaccactt tataaagtta
     7621 aaaaaaaacc gaatataata gtttttaatt atgacagatt ccattaactt caataataat
     7681 gacaatcata aagttctccc aaaggagacc tgcctgcagc cccgacaggt cgggataaac
     7741 caggcaagat cagggggacg ggttattatt ttttcagctc cttccggttc cggaaaaagc
     7801 actatcgtca atcatttgtt gcgatgtgat ttaggattgg aattttctat ttcagccact
     7861 tcacgtccac ctcgtggcaa tgaagccaat ggaatagagt attttttctt ttcaaaggaa
     7921 gaatttgaaa aaaaaataga aaaagatgag tttgtggagt acgaagaggt ctatccggga
     7981 tgttattatg gaacgcttcg cagtgaatta gaaaggattt cagctaaagg agcagtagtg
     8041 atttttgatg tggatgttat gggcggaatt aacctgaaaa ataaatttgg agatgatgct
     8101 ttagctgttt ttatagctcc accctccatc gaaatccttc gggatagact tataaaccgt
     8161 tgcaccgata gcgacgaaat gattgagaag agggtaggga aggctaagta tgaaatgggt
     8221 tttgcgcaag agtttgatcg cataatcgtc aacgataacc ttgaaaaagc taaaaaagag
     8281 gctgaaaaag tggtgcggga atttctaaaa gaagccccct aaacctgcct gccgccccga
     8341 caggtcggaa taaaccaggc agaaaccccc aagagggact tacagaactt gataataaaa
     8401 ttgagttgtt taatttgttt atatatgagt ccactccgaa aagtcataat ttataaaaat
     8461 ctaagatttt tatcctttgt gtcgaaatta gatgtgttga taatcaatta gatataaaat
     8521 tagtttcttt gtgtaacttg gtgttttagt gtctttgtgg caaaaacaag gttttcggag
     8581 ctgactctta tattattttc tgatattatt tgcaatccta tccggaactt ttttcttttg
     8641 tgtttttaat aagtttgaac tcttgcacta aatacttatt tttgcatcaa ccagaagtcc
     8701 cccttgcggg ggatttaggg ggctttacac taaaattatg aaatcaatcg gccttttctt
     8761 cggttcattt aatcctgttc atttgggtca taccagtctg gcagagtata tttttgaatt
     8821 ttcgggtgtt gacgaaatct ggttcgtggt cagcccgcgc aatccgctga aagagcaatc
     8881 ggagctgata gatgagcacc tgcgactccg gatgctgcat cttgcaactg aaaacacaga
     8941 atatctcgtg ccatcagaca tcgaatttga gcttccaaaa ccatcataca ctatcaatac
     9001 gctgaaaaca ctgagtgaac gattccccga agacaatttt attttgctga ttgggagcga
     9061 taatatgcag attttcgacc agtggaaaga ttatcagacc attctggatg attattcggt
     9121 gcttgtttat ccgcgcgaag gttattctta cgaagaattt gaagaaaagt atccggaaat
     9181 gcaaattctg gaagaagcac cgtttttcga tgtttcatct actgaaatac gtacacttat
     9241 aaaaaacaaa caggatgcaa gcccctggct gcatcccgaa gtgtatcagt ttatcagaga
     9301 taacggtctt tatactgatt aatttcttgt aattcgaaaa aatcgggaaa tttatattca
     9361 cagaaaagga ttatactgcc tttcgtatcc aatggtagta gacggtccgt gaccgcaata
     9421 cacaactgtt tcatcgggca gggtcatcag tctttcttca atggatttga tcagcgtagc
     9481 ataatcgccc tttacaaagt cggtacgacc aatcgaactg ttgaaaagta cgtcgccaac
     9541 gaaaaccact cccgcctctt tacaatacaa tgaaaagctt cctggtgaat gacccggagt
     9601 atgcatggct atcagcgacg tatttccgaa attaattatt tgattatcag taatatagct
     9661 tccaggacgc tgtacagatt ctgaataggg tattccccag tttttagcta ccagatgagc
     9721 agaatcaagc aaaaattcat cttcagcgcc ggcatcaggc attattccgt aggtttcatg
     9781 taaaaattta ttgccaaact gatggtcaaa atgcaaatgg gtattaatca cacgtttcaa
     9841 agtgagttga ttttcttcaa taaaccgctt aatccgttct ttttctttat caaacagcat
     9901 accggcatca atcacagccg cttcacctgt ttcatcccaa atcagatagg tgttctcctg
     9961 cagcggatta aaaggaaaaa cttttacgtt catatttgaa aaattagaaa ttagaagtca
    10021 gaagtcagaa attagaataa aaaataagca caaattttag agaattttca ctcaataata
    10081 taaagtagaa atgctgcact tttcatttcc cttgcggggt aagggttagg aataagggct
    10141 ttctaaagag gcaaataaac aatctttttt gtttcgaaaa attctccgga aaaaaaatcc
    10201 ttcaatgcat acgtttctgc tgtatttctg aaaggcagta tttcgtgttc aagttcgccg
    10261 cctttcagac agattaaacc attcgggagt gcattttgtt gtttcggact tatgtttttc
    10321 cggcagagct tcaccagttc ggcaagaggc ataactgccc ggcttaccac aaagtcgtat
    10381 ttgttttttt cctcctgaat tctcgaatgt tttaattcaa tgtttttcaa ttgaataaaa
    10441 gatgaaatct cctgtgtaac tttaatcttt ttaccaatgc tgtcaatgag tgtaaaatga
    10501 acttccggaa aaaatattgc cagcggaata cccggaaatc cacctccggt gccaacgtcc
    10561 aaaacccttg tacccgcttt gaagtggatg acttgggcaa ttccgagtga gtgcagcacg
    10621 tgtctttcat acaaattatc aatatccttg cgtgaaatca cattgatttt actgttccaa
    10681 tctacatata aatcatacag agagccgaat tgcgacacct gagtttcatt taaatttgta
    10741 aagtatttca gaattaaatc catacacatt tatttttttt tgtgttttca aaagtacttc
    10801 attttttgca gatactcaaa ccgcttataa aacattttca gaatggatta aaatttgtaa
    10861 ctttgcaccc gttttttgaa taatcaatta atcagaataa atacaaacta aataagcaac
    10921 ttagaaaatt atgagagcat acatatttcc cggtcaggga gcgcagttta caggcatggg
    10981 aaaggatttg tacgaaaatt cgccattagc cagagagtat tttgaaaaag ccaatgaaat
    11041 tcttggttat cgcattaccg atttaatgtt tgaaggaact tccgaagatc ttcgtcagac
    11101 caaggtaact caacccgccg tttttcttca ttcagtaatt tcggccattg ccctgggcga
    11161 taagtttaaa cccgatatgg ttgccggtca ttcactgggc gaattttcgg cactggttgc
    11221 agccggagca ctaacttttg aagaagggct cagactcgtg tacgctcgtg caatggccat
    11281 gcagaaggca tgtgaaaaag aaccttcaac gatggctgcc gtactcggac ttgacgacga
    11341 aacagtggag gaagtttgcg acgaggtgaa taaagaatac attgtggtgg cagctaatta
    11401 caacagtccg ggacaattag tcatctcggg atcaatcgaa ggaattgaca aagcctgtga
    11461 attgctgaaa caacgcggtg ccaaacgcgc cctgaagctg cccgtagggg gagcattcca
    11521 ttcacccctc atggaacctg ccagacagga actccaggct gccatcgaaa aagcagttgt
    11581 aagcactcct gtctgccctg tatatcagaa tgtgaacgca aatccgcaaa cagatccgga
    11641 gtcgatcaaa aaaaacctga tagcacagct caccgcaccc gtacgctgga ctcaaaccgt
    11701 aaaaaatatg atcaacgacg gagctgatga atttatcgaa cttggcccgg gcgatgtgct
    11761 gaaaggtttg gttaaaaaag taaatccgga tataatcacc ggataactca ttttttttca
    11821 aatacaatta aaaaagcgct gtagtaaatt ctgcaacgct tttttattgt ttttttatac
    11881 cgttttattg ctattttttt tatacctttg atgtgaaaat taaattattt tttttacaca
    11941 tttaattcca tctaacgcat taaataacaa atgtttatgt caaaaaaact tttaatcaga
    12001 gacctcacat tacgcgatgg tcagcagtcg tcttttgcaa cccgcatgag tcaaccacag
    12061 attgaccggg tattacctta ctacaaggaa gctaattttt atgccatgga ggtttgggga
    12121 ggagccgtgc cagactctgt gatgcgctat ttgggcgaaa atccgtggca tcggttggaa
    12181 aaaatccaca aaggagtgga aggcgtatcc aaattaacag ctctgtcgcg cggcaggaat
    12241 ctttacggtt atgcaccata tccggatgaa attatcgatg gtttctttaa aaatgctgta
    12301 agttcaggat tggatatcat gcggattttc gatgcgctga atgacgtcga aaatataaaa
    12361 tcaagcgtaa aatcgatcaa aaaatacggt ggaatggctg attgcgcagt gtgttatacc
    12421 attgatccaa agtatgatga tgaaataaaa attgtagaaa agaaaggttt tctgggcctg
    12481 ttcaataaaa aagaagaagt tgtagtgaaa agacaaccag ttttcaccga tacttatttt
    12541 ctgaataaag ccaaagaaat ggaagctctc ggagccgata tgattaccat caaggatatg
    12601 agtggcctta taccaccctc acgagtggct tctctgatga cgttgttcaa aaaagaactg
    12661 aaagtacccg ttgatttcca tacgcattgt acaccaggtt tcggcttagg ttctgtactc
    12721 actgccatta tgtatggtgt ggatgtggta gatacaaata tatggtactt ttccggaggt
    12781 cctgccgcac cggcaatcga actgatttat cttttctgta aaaaactcgg tattgagctg
    12841 gatgtaaaca tgaaagctgt agccaaaatc aataaggagc tggaaggaat ccgcagggaa
    12901 ctggccgctt ttgataccgg aaagcacttc ccgaaagcat ttaatccgct tactgataaa
    12961 ctaccggctg aaattgacaa agaatttgac aaagccatcg aagcagtgaa gaaagaggat
    13021 gaaaatgctc tgctggctgc ctgctacgct atcgaagctt actttgaatt cccgaaaccg
    13081 gacgatctgg ttcgtaaagc ggaagttccg ggcggaatgt acaccaacat ggtggcacag
    13141 ctgaagcaat tcaattcaat ggatattctt gaagacgcct tacacctgat tcctagcgta
    13201 cgtttggatt ccggattacc tccgctcgta actccaacca gtcagattgt tggtgcacaa
    13261 gctgtagctt ccgcactgaa cattaaaaac ggaagaccga aatatgctaa tccgtccaat
    13321 cagtttattg cccttgtgaa gggagaatac ggaaaaactc cggttcccgt tgatccaaag
    13381 ttccgggaac aaatcaccgg ttcacccaga gaaatgccct atgatacaag tggttacaaa
    13441 cgtcaggata atcctgtatt gccggaattt ggcaatgtaa aactggcaaa ggacgaaaag
    13501 gacgaattgc ttctggaatt attcccatca gtagctacta actacctcag gggcattcgt
    13561 gaaaacgaat ataaactcaa tccgcagcct gctcaggtag ctgaaaccaa agaaactgta
    13621 aaaaaagaaa ctgttactga agttcagcag cctgtaggga aaaaaatgaa atgtccgctt
    13681 ccgggtacca ttttgagcgt gaaagtaaaa gtaggcgatg tagtgaaaga cggacaaacc
    13741 ctgttcattc tggaagctat gaaaatggaa aacaacatta cttccgaatt taacggaaaa
    13801 gtgattaata tcttcgtaaa acccggtgaa atagtacctg aaggtgctcc attggttgaa
    13861 attgcccacg aataatacag gaactattcg tttgaatgat atttttcaga tactaaaaat
    13921 gcagaaaact ggttttctgc atttttttat gctcatacaa aaattccatt gatacaaccg
    13981 atgagttcaa ttaaaaatga atggagtagg gggagtattc attctttttt cttaattttg
    14041 caattcaaaa aaaaacttag ataacatgtt cagaaataaa acttgcggcg agcttcgact
    14101 tgccgatgcc ggaaaaaacg taacacttgc cggctgggta cagcgaacac gtaaaatggg
    14161 cggaatgacc ttcgttgatt tacgcgacag atatggcatt acccagctgg tgttcaatca
    14221 ggatgtgaat gccatactct gcgaacaggc taacaaaatg ggacgtgaat acgtgataca
    14281 gataaccgga acagtagcag aacgaagcaa taaaaactca aacatcgaaa caggcgaaat
    14341 tgaaatactg gtggatacgc tccggatact caatgaatcc aaaacaccgc cctttaccat
    14401 tgaagacaat accgatggag gcgatgatat ccgaatgaaa tacagatacc tggacttgcg
    14461 gcgaaacagc gtacgggcta acctggaact tcggcataaa atgaccattg cggtgcgtca
    14521 ataccttgac agcctcggat ttctggaagt ggaaacgccc gtactcatcg gatcgacacc
    14581 cgaaggtgca cgcgactttg tggtgccttc acgtatgaat ccggggcagt tctatgcctt
    14641 gccgcaatcg ccccaaacac tgaagcagct gctcatggta tcgggtttcg accgatattt
    14701 tcagattgta aaatgttttc gcgacgaaga cctgcgagcc gaccgccagc ccgaatttac
    14761 gcaaatagac tgtgaaatgt cgtttgtaga acaggatgat gtgttgaata ctttcgaagg
    14821 acttaccaaa catcttttca ggtcactcaa agggttagaa atcagtgaac catttttgag
    14881 aatgacatgg gccgacgcaa tgaaatacta cggctctgat aaacccgaca tgcgttttga
    14941 aatgaaattt acagaactga aagaaatcgt ttcgggtcat aatttcagcg tatttgacga
    15001 tgcggaatac gtggcaggaa tctgtgctga gggatgtgcc ggttacacaa gaaaacaaat
    15061 cgacgaactg accgattttg tgaaacgtcc gcaaatagga gctaagggac tcgtttatat
    15121 ccgttacaac gaggatggta ctctaaaatc atcaatagat aaattcttta ccgaagatag
    15181 cctggctgga attgcttcac agtttaatgc tcaaaaaggc gatttaatac ttatattggc
    15241 aggtgataag cgcaaaactc agaaagcact atgcgaattg cgattggaaa tgggagagag
    15301 gttaggcctt cgcagcaaag ataagtttgc tccgctttgg gtggtcgatt ttccgctctt
    15361 tgaatgggac gaagaagagc aacgctattt tgctatgcat catcccttta catcacccaa
    15421 accggaagat attcatttac tcgacaccaa tccgggagaa gttcgtgcca atgcgtacga
    15481 tatggtaatc aacggggtag aattaggggg aggatccata cgtattcacg atagccagct
    15541 acagtcaaaa atgtttgaat tactgggctt tacgccggaa aaagctcagg cacaattcgg
    15601 atttctaatg ggcgcattcc aatatggagc acctccacac ggcggactgg cttatggact
    15661 ggatcgcttc gtatccatgt ttgcagggct tgacagcata cgcgactgta tcgctttccc
    15721 gaaaaataat tctggacggg atgtaatgct cgacgcaccg actgaaatcg atcaaagtca
    15781 acttgacgaa ttgaatctga ttgtaaatct taaaaaataa aaattattga atagtcctga
    15841 aagtcctgcg aaattcagga ctttttttta tttcctgaaa aataccaaaa gaggtgaatg
    15901 tcaatttttc aaaagagcat aaattgcatg ttcatttaat tgaaacagga atttctgata
    15961 aatagaataa attttatagt aatagggaac attcataaat tatatttgac ataatataaa
    16021 ttatgagaaa attgtggttg attttttgaa ttgtcagatg gtatcaatct cggggcggtc
    16081 aaatcaggta aatttatcaa actgatggtt cgatcgcatt gtgtaatttc aatattcagg
    16141 agtaagtatg gttctgccgg cattcactcc aatctcagag cgtcaaacaa tgtttttttt
    16201 ttctgaaaaa tcattgttgt ccgtttaaaa tacttggaac tttgcaatgc tgttgtagtt
    16261 cagggcgcag agcgactaat taattgcaga aaatcagaat aagcactaaa aaagctacag
    16321 agtagctaaa tatgaatttt tatcagataa taacgaaaaa agaatattta aagtgctaat
    16381 tattaaatgg ataatatgat gtagaaaaat aaaaagggga gcggtatcaa taaaaaatca
    16441 tcacccccct actccattta ttgctaaatt tttatggcaa acttctttaa cctggaatcg
    16501 agcatatgtg gtggtataat tttcttaact gtttcagcta cagcgttcaa ctgtccttcg
    16561 cggataaaat tttgctgaat gatgagcgat atttctcttg ccggctcagg ttctaccagt
    16621 ttacgcagat ttttcttctg tatgtcagtc agaagttcca catgaagttc gggaattacc
    16681 gtataacctc cgttttcatc cactattttt acgagcgtat caatactgcc cgattcatag
    16741 gcatatgaag tgacaatacc actgttgcaa aagttaaata cctgattacg aagacaatga
    16801 ccttcttcga gtacccacaa atgttccagt ggaagatcac ccgaaataag ttcggtgtgc
    16861 ttatacagtt cgttattcgg tgatatgtaa gctataaatt tctcgtaata aagaggtatc
    16921 tccagtacgt tgggattgct gagcggcgta gaaagaattg ccatgtcaat atcacccagc
    16981 atcaacttac gttcgatggt atcggtgtgc atttcataca ttttgaatga caactgcgga
    17041 taattccgtt tgaattcggc aaagaatttt ggcaacagat agggtgccac cgtaggaatg
    17101 atggccagat tgatatggcc ggttaaatca cctttttcgg attcaaccat ttcattgatt
    17161 ttattcaact ggcacagcac aacctgtgcc tggtcaacta ttttattacc tattgcagtt
    17221 attcctatcg gatgtttcga ccgatcaaat atcttcacat ccagctcatc ttcaagtttt
    17281 tgtatcatca tactcaacgt tggctgagtt actccactca tttctgctgc ctgtacaaaa
    17341 tgctttgttt tgctgagcgc cacgatatat tcaagctgtt gaagtgtcat aaaataaagg
    17401 ttattttaat aatttcacaa agatatagat tatttttatt aaaaatcgaa tatttcgatt
    17461 gataaatagc taaaagttgc gaaaatgtta atttcaaatt tgctcaatga atcatttcat
    17521 ctacttttgt ttcaaatttc aaaaaaaaat aatgaatcga atcaaactac tgattttact
    17581 tgtaataatc tgtatatcaa ctgtcaacgc tcagttgctc tggaagataa gcggacacgg
    17641 tctgaaacaa aactcttatc ttctgggcac tcatcatctt atctcaatca gctttctgga
    17701 cagtattccc ggagtatatc ctgcattcaa taaatcaaaa atggtggttt cggaaattgt
    17761 attcagcaat caggatgtat cagccaaaat ccaaaaggct tctaccctgc ccgattctgt
    17821 tacgatgaaa aatttattat ctgctgaaga ttttgatttt gtgaataaag aattgattga
    17881 tgtattgaaa atgaacttgt taagtatcaa taaaatgcat ccttcgctta ttcagacatt
    17941 ttatgaactg gaattgtaca aagaaagagc acattttgac ccaaacacaa aatccgattc
    18001 gtattttcag ttggtagcca gccaaaaagg aattccggtt gcagggctgg agactgtgga
    18061 taagcagatt gaattgcttt ttccgaaaga caatctgaaa aagagcgctg aggaacttgt
    18121 aaaaaccatc aggaaaaaag atttgctgtt tgccgactac cagcagttga ataagctgta
    18181 tcgatcaggt gatattgaag gtctgagtga aatgaacaag gcatggaaca gagaatgggg
    18241 ggtaactgcc gaagaaaacg cccgtcagct ggatttacgt aacattgaat gggtaaaaca
    18301 acttccggag ttgatgaaat caaattcgtg ttttattgct gtgggagcac ttcatctgcc
    18361 gggtgagaac ggcttgattc atttactccg gaaaaaaggt tataaagtga aaccggtgaa
    18421 ataacagaag gattgataag ttatacggaa taattatcgt ctaaatgtct tattatataa
    18481 caaattatta ctaaatttgc acttaattga aaaaaaaaca tgtaaatatt tatattctat
    18541 gagcaaaatg aaaaaatttc tgttatcaga aaatgagatt cctacacaat ggtacaatat
    18601 cacggctgaa atgccaaaca agcctcttcc gttgattaat cccggtacaa aagaagcctt
    18661 aaagcctgag gatttatttc caatattctc acaggaagtt agcagacagg aaatgaataa
    18721 caccgatgtg tggattgata ttcccgacga agttcgtgaa aaatacatga tttaccgccc
    18781 taccccgctt gtacgtgcct ataatctcga aaaagcactt gatacgccgg ctcatatcta
    18841 tttcaaaaat gagagtgtaa gtccggtggg atcccacaag cttaattcgg cacttccaca
    18901 agcctactac tgtaaaaaag aaggcataac taacgtaacc acagaaaccg gtgccggtca
    18961 gtggggtacc gcactcgctt ttgctaccaa agcatttgga ttggagctgg ctgtgtacat
    19021 ggtgaaaatc agttacgagc agaaacctta tcgccgttcg ctgatgcaga cgtggggcgc
    19081 acaggtgatt gcatcgccaa gtatgtcgac caaagcaggc cgaaaaataa tcaccgaaca
    19141 tccaaactat cagggaagcc tgggtacggc tatttcagaa gccatcgaac tggctcttag
    19201 cacaccgaat tgccggtata cgctgggaag tgtgctcaat cacgtaatgc ttcatcagac
    19261 agtcatcggg ctcgaagccg aaaaacagat ggaaatggcc ggagagtatc ccgatgtagt
    19321 aatcggatgc tttgggggcg gttccaattt ctccggaatt tctttcccgt ttatgcgaca
    19381 caacataaaa aacaatgcca aaactcgatt tatagcagct gaaccggctt cttgcccaaa
    19441 acttacccgt ggagttttcc aatatgattt tggtgatgaa gcaggttata cacccctcat
    19501 cccgatgttt acgctcgggc ataattttgc gcccgcacat attcatgcag gcggattgag
    19561 atatcacggc gccggaagcg tggtaagcca gcttatgaaa gacggctaca tggaagccat
    19621 ggatatccct cagcttgagt cttttgaagc aggagtttta tttgcacaaa cagaaggaat
    19681 agtaccggca ccggaatctg ctcatgccat tgcatcggca attcgcgaag ccaaaaaagc
    19741 gaaagaagaa ggagtaaaaa agaccatttt gttcaatctt tccgggcatg gatttgttga
    19801 tatgtcagct tacgaccagt tcctggccgg cgatttgaga aactatgaat taaccgcaga
    19861 cgacatagct aaaacaactg aaatactcga acgtattgta taattattca aaagtatccg
    19921 acacttaata atccacgtgt cccactcttt tagctctccg ttttctgcgg ggagcttttt
    19981 tttgtgatag tatgacaaaa taaagaatat ttaactttca gtatatttca attccttatg
    20041 gttcgattaa aagtcaatca gatttgcagt tccaactac
//
LOCUS       DAORKP010000021        19686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_129_length_19686_cov_8.020936, whole genome shotgun sequence.
ACCESSION   DAORKP010000021 DAORKP010000000
VERSION     DAORKP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19686
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_19686_cov_8.020936"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLN75_02335"
     CDS             <1..1110
                     /locus_tag="PLN75_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG02986.1"
                     /translation="DAMTMGKHVYCEKPLTHSVYESRLLTNLTRKHKVATQMGNQGSS
                     GPGVRQVVDWIWAGEIGEVKKVEVFTDRPIWPQGLNVPKEVHKVPKTLNWDLFLGPAP
                     TRPFNEIYHPWNWRGWWDFGTGALGDMACHMMHPIFKALKLGYPTKVQGNSTLLLTDC
                     APSAQTVKYTFPARDNMPKVAMPEVEVYWYDGGIQPMRPAGMPAGKDLNDDGGGVIFH
                     GTKDTLICGCYGVRPWLLSGRTPASPKTQREVTTSHEMDWARACKEDADDRTETASNF
                     YEAGPFNEMVVMGVLAVRLQSLNQELHWDGENMRFTNIPAGATIKTVIEDGFKITDGH
                     PTFNKTWTDPVDATAFAEGLIKHNYRAGWNLPAMP"
     gene            1166..>1995
                     /locus_tag="PLN75_02340"
     CDS             1166..>1995
                     /locus_tag="PLN75_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRG02987.1"
                     /translation="MKKPVFILSLLLLTVSFVACGGNTKKTEAKSENSETMNAAVPEY
                     TVVDKPQVDLTTFTTDKDGYIVIFNGKDFTGWRGYGKDSVPGKWTIEDGAIKFNGTGG
                     GEAQDLEGGDIIFGHKFKNFELTLEWKVSKGGNSGIFYLAQEVSTKDKDGNVKMEPIY
                     ISSPEYQVLDNANHPDAKLGKDNNRQSASLYDMIPAVPQNQKPFGEWNTAKIMVYKGT
                     VVHGQNDANVVEYHLWTPQWTEMLQASKFSQEKWPLAYELLNNCGGANREGYIGLQDH
                     G"
     assembly_gap    1996..2095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2096..2638
                     /locus_tag="PLN75_02345"
     CDS             <2096..2638
                     /locus_tag="PLN75_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRG02988.1"
                     /translation="WKWWDTAIAAHKAAGMKYIVKPWMPVPKTLADLKAYCDYYNQVG
                     EKCNAAGLRFGYHNHDFEFAKIEGETMYDYMLKNTDPAKVFFQMDVYWTVMGKKGPVD
                     YFNEYPGRFEILHIKDNKELGQSGMVGFDAIFNNADKAGAKYMVVEVEKYNFEPLKSI
                     KMSYDYLNRNAFFKNDYSVK"
     gene            2903..4105
                     /locus_tag="PLN75_02350"
     CDS             2903..4105
                     /locus_tag="PLN75_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRG02989.1"
                     /translation="MSERILSALNVKSSKKPIPDLLSGIYDQDKYQKQQSYFRANTRF
                     GLLTSTVSFLVIILMIVFGGFGWLDGIVQRWTANPILVSLIFFGILFLANDIISTPFE
                     IYDIFVIEERFGFNKTTPKTFILDKLKGYALTAVLGGGIIALVMWFYQLNPQYFWLIA
                     WACVTSVSLFISLFYSELIVPLFNKQTPLEAGELRDAIEKFSKKAGFELKNIFVIDGS
                     KRSTKANAYFTGFWKKKRIVLYDTLINDLDTAEIVAVLAHEAGHSKYKHTLRNILINL
                     PYTLLVFFLLGLILRSDVLAQALGGKEASFHLNILAFGVLYSPVSMMLGLAMNVLSRK
                     HEYQADGYVKENKMGEQLISALKKISSKSLSNLTPHPAYVFVNYSHPTLLQRVEKLTN
                     SPVNQSTN"
     gene            4115..4858
                     /locus_tag="PLN75_02355"
     CDS             4115..4858
                     /locus_tag="PLN75_02355"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRG02990.1"
                     /translation="MIGLMGAMPEEMNRIIGAITEKSITELGNRLYHTGKLFGKEVVA
                     VFSRWGKVAAATTATNLILEYKVDQIIFTGVAGAISAELNIGDVVIAQRLFQHDMDAR
                     PLMRRFEIPLTGKTSFEIPDENTRQMADAVHHFLLNNREFRKRLAENDIKNPKMMLGD
                     IASGDLFVSGTEMKNELIRNLPSVVCVEMEGAAVAQVCYDFGVPLQVVRVISDTADEQ
                     AHTSAIGFVNQHGGEYSLAILREYFKKMD"
     gene            4939..7146
                     /locus_tag="PLN75_02360"
     CDS             4939..7146
                     /locus_tag="PLN75_02360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRG02991.1"
                     /translation="MENTSFYLVPLSSLLALGFAFYFFRSMMKHSEGTDTMKRIAQYV
                     REGAMSYLKQQYKVVTIVFIVLAIIFGVMAYFGLQNSWVPFAFLTGGFFSGLAGFFGM
                     KTATYASARTAFAASKSLNHGLRIAFRSGAVMGLVVVGLALLDISLWFVILNYFIPDA
                     DPGHKLIIITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPR
                     NPATIADNVGDNVGDVAGMGADLYESYCGSILATAALGASAFMTQPEMQLKAVFAPML
                     IAAAGVVLSILGIFIVRTKEDADMKKLMAALNRGVNTSALLITGATFLILYLLDIPNW
                     VGLSFSVIAGLVAGIIIGQSTEYYTSHSFKPTQKIAESSGTGPATVIISGIGTGMLST
                     AIPVVTIVAAIVISFLSAINFDTAGMLTAENLSKGLYGIGIAAVGMLSTLGITLATDA
                     YGPIADNAGGNAQMSELDPEVRRRTDALDALGNTTAATGKGFAIGSAALTALALLASY
                     IEEVKIGLVRLGRTTLQLANGDVIETTKASFVDFMNYYEVNLMNPKVLAGAFLGSMMA
                     FLFCGLTMNAVGRAAQKMVNEVRRQFREIKGILEGKETPDYARCVEISTIGAQHEMLI
                     PSLLAIVAPTLTGLLFGVAGVMGLLAGGLASGFVLAVFMANAGGAWDNAKKYVEEGHY
                     GGKGSENHKACVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLIVSWSLL"
     gene            complement(7214..8728)
                     /locus_tag="PLN75_02365"
     CDS             complement(7214..8728)
                     /locus_tag="PLN75_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRG02992.1"
                     /translation="MLLPSDEYILKQRGITKQQIENQLLSFVNGFPFLEIRSAASINN
                     GITVIPAENQHKLLNEWEIYQQSDAVVLKFVPASGAASRMFKDLFSFLEKDENTPSSD
                     FEKKFFDEISHFAFYDDLNEACIKNEGRTIPELMAAGQFKDVVSDLLNSNGLSYGSLP
                     KGLLKFHSYPDDKRTPLQEHLVEGALYAKSNGKVNIHFTVSAEHRVLFEKHLLESLPK
                     YEKQYGLKYQVSFSEQKPSTDTIAADSDNQPFRDRGELVFRPGGHGALIENLNDLEAD
                     VVFIKNIDNVVPDSLKEATVTYKKLIAGLLVHLQKQTFAYLHELENEGISDAKLAVIS
                     QFCESKLNNHHPDIRSLKGNELRQYLFNKLNRPIRVCGMVKNVGEPGGGPFLTVNQDG
                     TVSPQILESSQINQNNPEDKAKMLNSTHFNPVDLVCGIKNYKGEKFNLLNFVDKNTGF
                     ISLKSKSGRELKALELPGLWNGAMSDWNTVFVEVPIETFNPVKTVNDLLRKEHQ"
     gene            complement(8755..9792)
                     /locus_tag="PLN75_02370"
     CDS             complement(8755..9792)
                     /locus_tag="PLN75_02370"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HRG02993.1"
                     /translation="MKTKILKFILPISLILILSSCVTPRMFTTLDVLRPAEVTFNPEV
                     QNVLIVNNSVVQPYNVGHADIKSSATRNNPVNVSLKFDSAALFCAASLRENLEAKDFF
                     YSVSMSQTNMNTTDNYYRLAPLNKQTVRFLCGLYNADALISLDHIQTEDKSYMNSGNI
                     QSALDVKINTKWTIHYPTDSLSVFKEFSDEFSWEDAQFNKLPNRYDALVDACILTGSN
                     IADRMIPRWEKEDRYFYTPKKQLFVQAMDSLTYKKWQAAIDLWVKASETTKNNKTKFH
                     AYNNIAIAYEIMGNLDKAMNYASKAVETFPNIIAFSSTTLDEIYELLDYYEILKKRKQ
                     EAILLDKQLND"
     gene            complement(9830..10645)
                     /locus_tag="PLN75_02375"
     CDS             complement(9830..10645)
                     /locus_tag="PLN75_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRG02994.1"
                     /translation="MNNFTEYNNPDKKLQAVSAAGYESNITFLGTGTSTGVPQLGCNC
                     KTCCSTDPRDKRLRCSALITVQGKRFLIDAGPDLRQQLITNKIFHIDGILLTHEHYDH
                     VGGLDDVRPLGEVKIYAERKVLNAIQRNMPYCFGKYKNPGVPSLDLYEIDTGTFGAEG
                     VKITPVRIMHARLPILGFRIGDIAYLTDVKTIPETEFEKLKGLKILVVNALRKEEHFS
                     HLSVDEAIRIARKINADSSYFTHASHHLPVQSEIEKELPQNIHFAYDNLSINF"
     gene            complement(10694..12064)
                     /locus_tag="PLN75_02380"
     CDS             complement(10694..12064)
                     /locus_tag="PLN75_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HRG02995.1"
                     /translation="MNLPAPFTAAMKSLLKDKYTSFADSLNEPSPVSVRVNDKLQLIP
                     SACKVAWCDSGYYLDERPLFTADPLFHAGAYYVQEASSMFLQQVLEQFASPGSTVLDL
                     SAAPGGKSTLISRFLNKKGFLVSNEIVRSRAYILSENMIKWGNDNFLVCNNAPEDFKR
                     LPSFFDVVVVDAPCSGEGMFRKDAGAIDEWSEQNVVVCAARQKDILIGVWDTLKTDGI
                     LIYSTCTYNEAENEYNVKWMEDELGAEVLHADISNFPEITATEKGCRFFPHKTRGEGF
                     FISVVRKTSPAPRTPTGRKKSAKSPVKAEHSFHLLKNQLLDSENRELIFENNLISAIP
                     KEHAEKVDLLRNNLKTMHYGITLAEQKGKDLIPHTSLALSKSIDIKKMITAEVDITTA
                     IQYLSKEAIALPETAKGYVLLTYESVPIGWVKNIGSRCNNLYPAEWRIRMKIQHSTPL
                     HWIRKV"
     gene            12204..14465
                     /locus_tag="PLN75_02385"
     CDS             12204..14465
                     /locus_tag="PLN75_02385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRG02996.1"
                     /translation="MRIRSLFIFLTTGILVFSSCSKEYITDYTSLVDPFIGTGGHGHT
                     FPGATYPYGMVQLSPDTRIFEWDACSGYHFTDTTINGFSHTHLSGTGIGDYTDVLVMP
                     VVGEPDIRKATPESQQTSFASPFRKETEKAVPGYYTAELDRYKVKAELTATQRAGFHK
                     YTYPLSDKAGLLIDLDYTNQNHPTDSVIIEIVSDTEIRGLKKSRGWARQQWIYFYAVT
                     SKPFTSATFYKNDSIVPDSMLLNGKSGKIFLSFNTKDREEVMLKVGISAVDIDGARKN
                     LEQEIPAWDFEGVRINTRKVWNDLLAKVDVESKNETDKRIFYTALYHTAIAPNVFSDV
                     DGRYRGMDKQIHQSGENVYTVFSTWDTMRALHPLLTIINPELNQNFIRTLLSKYKEGG
                     ILPMWELAANYTATMIGYNAVPIIVDAYMKGDRNFDLDLAYEAVLKSSAYDTTGIKDT
                     PLYINALQPISKKFKNETGYIPFDKEIESVAKGLEYAYDDWCILQMARDRKDTANITK
                     YEKLATNYRNYFDAGTGFMRGKDSKGNWRSPFNPRSSVHRDDDYCEGTAWQWLWFVPH
                     QVDSLVQLLGGKEKFVAKLDGLFTADSRIEGNQTSVDISGLIGQYAHGNEPSHHIIHL
                     YNYVDMPYKTQELIDTVLYSQYSDKPDGLSGNEDCGQMSAWYVLNSMGFYQLCPGNPT
                     YSIGRPVFDNVRITLNNGRYFEIKTYNNSRKNKYIKSIKLNNKKLEKPFFTHAELMAG
                     GKLEIVMTDKAKK"
     gene            14591..15748
                     /locus_tag="PLN75_02390"
     CDS             14591..15748
                     /locus_tag="PLN75_02390"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRG02997.1"
                     /translation="MKTLQVKVNDNPLTLPVITLNGNDVLEISFDEMSHEKRSFSYEI
                     KHCNADWTISSLSSSEYLQGFSRGYIDDYALSVNTTFLYTNYRFILPNNDVSFAVSGN
                     YLINIYEDNQTDNPVAHVCFSVVEPQVEISAAVRGNTDTELNKSMQQLDFDVVMRNYK
                     IQDAQTELKVVVRQNNRTDNQVTGLKPTFYSADKLSFVNNRQLIFEGGNEFHRFDFSS
                     IYNYDERIAQIKFDRPYYQVYLTENSIQADDAYMQDFDVNGRFVVNYQNAIESPDVEA
                     DYMFVNFYLPVNRPFIQGNIFLGGNWNYNRLNALSRMDYDPSNGLYYKTLLLKQGGYN
                     YQYLFLREGETRASTKPVDGSHWQTQNEYAIYIYHRPWGGRYDKLVGVKIL"
     assembly_gap    15755..15854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15895..17163
                     /locus_tag="PLN75_02395"
     CDS             15895..17163
                     /locus_tag="PLN75_02395"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRG02998.1"
                     /translation="MIVYKFGGASVKSSDGIKNIVQIVSSVQDDLFIVVSAMGKTTNA
                     MEVVLDFFMKAEREAALVKLTEVESYHQQIISELFDDKTVGEKAVEPLFDQLKHFLNE
                     AVGNDYDRWYDQLVSYGEVISTAIVSAFLQESGVENKWLDMRELLVTDSNFREANVNY
                     LESEKRLKAAVDFSKHKVYIGQGFIGTTMKGLPTTLGREGSDYTAAVVGNLLDASGVT
                     IWKDVPGILNADPRIFPNTVLIPELTYMDAVELAFSGAQIIHPKTIKPLENKKIPLYV
                     RPFGSPQEAGSVINNSAPPIQVPVLILKKNQVLVTLRPLDFSFILEESLTWIFTVVEK
                     HRLKVALIQSSAVSISICVDNSRYLQSALDELANDFKVSYNTGLELLTIRGTNPDIIR
                     RTTEGREILVQQNTRRIGRFVMKEKPENGG"
     gene            complement(17725..18654)
                     /locus_tag="PLN75_02400"
     CDS             complement(17725..18654)
                     /locus_tag="PLN75_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrr restriction system protein"
                     /protein_id="HRG02999.1"
                     /translation="MNNNLQKLAPSKQSAAKTIFATFNILKEAGGQLPGKQVIERIRD
                     SVELSDWELEVYEKTGYVRWESFLHFYTIDAIKAGYLRKNKGIWYLTDEGEKAISLGP
                     VKLLETASQFYRSWAADKKGNKSIKSKDQENEQTELEENKTQTQKANLDLLEERAIAG
                     IKDFIRSKNAYEFQDMVAALLRAMKYHTPFISPKGKDGGLDIIAYNDPLGATSPRLKV
                     QVKHRPDSSVPVDDIRSLTGLLNKDGDVGLFVTSGSFTSEAERSARESHRHIKLLDID
                     NFIALWQEFYAKMTDEDKNMLPLYSIYFLGSND"
     gene            complement(18767..19210)
                     /locus_tag="PLN75_02405"
     CDS             complement(18767..19210)
                     /locus_tag="PLN75_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03000.1"
                     /translation="MKNLKQFFHTLSYIQYPLLLIGLYLIVKPILKGFDYLSANPEYL
                     FGTYNNALIFLGLTLSFAALQDSSRTSLKYEKRIWSNPKRAKTTILFTLISMFVFLIA
                     GLLGFIVKTSLFREFSYGSIILAIGLLGYLKLQLEIFENQKERKL"
     gene            complement(19342..>19686)
                     /locus_tag="PLN75_02410"
     CDS             complement(19342..>19686)
                     /locus_tag="PLN75_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRG03001.1"
                     /translation="GDILTDEASVITGSLGMLPSASIGLHTSVFEPIHGSYPQAAGKD
                     IANPLATILSAALMFEYAFGMMEEGKLIRDAVTASMDAGIVTEDIADGAKAYKTSEVG
                     KWIVEWIKSSEL"
BASE COUNT         5423 a         4029 c         4390 g         5644 t
ORIGIN      
        1 gatgccatga caatgggaaa acacgtttat tgcgaaaaac cattgactca cagcgtgtac
       61 gagtccagat tacttactaa tctgacccga aaacacaaag tggccaccca aatgggcaat
      121 cagggctcat ccggacccgg agtacgtcag gtagttgact ggatctgggc cggtgaaata
      181 ggtgaagtaa aaaaagtgga agttttcacg gaccggccta tctggcctca gggactgaac
      241 gtaccgaaag aagttcacaa agttcccaaa acactcaact gggatttatt tctcggaccg
      301 gcacctacac gtccgttcaa tgaaatatat catccatgga actggcgcgg ctggtgggat
      361 ttcggtacag gtgcgctcgg cgatatggcc tgccacatga tgcatcctat tttcaaggca
      421 ttgaaactgg gatatccaac caaggtacag ggaaattcca ctctgctgct gactgattgt
      481 gctccttctg cacagaccgt gaaatacaca tttccggcac gtgataatat gccaaaagtg
      541 gccatgcccg aggtggaagt gtactggtac gacggcggaa ttcaaccgat gcgtccggcc
      601 ggtatgcctg ccggaaaaga tttgaacgac gatggcggcg gcgtaatttt ccacggaacc
      661 aaagatacat tgatttgcgg ttgttacggt gtgagaccct ggctgctctc gggcagaact
      721 cctgcttcgc caaaaacaca gcgcgaagtg actacatcac acgaaatgga ctgggcacgc
      781 gcatgcaaag aagatgccga cgaccggaca gaaaccgcat ccaactttta cgaagcaggt
      841 cctttcaatg aaatggtagt aatgggagtg ctcgcagtga gattgcagtc gttgaatcag
      901 gaactacact gggacggtga aaatatgcgt ttcaccaaca tccccgcagg tgcaaccatc
      961 aaaactgtga ttgaagatgg attcaaaatt accgacggac accccacttt caacaaaaca
     1021 tggacagatc cggtggatgc taccgctttt gccgaaggcc tgatcaaaca taattaccgt
     1081 gcaggatgga atcttccggc aatgccctga ttcaatgtac tatttaatga tttacaattt
     1141 accatttttt aaaaaaatat tgaatatgaa aaaaccagtt tttattttaa gtttattact
     1201 attgactgtg tcttttgtag cttgcggagg caatactaaa aaaacagaag caaaaagtga
     1261 aaacagtgaa accatgaatg cagcagttcc tgaatatacc gtggttgaca aaccccaggt
     1321 ggatttgaca acctttacta ccgataaaga tggttatata gtcattttca acgggaaaga
     1381 ttttaccggt tggcgcgggt atggcaaaga ttccgttccg ggaaaatgga ccatagaaga
     1441 tggtgccatc aagttcaatg gaacaggagg aggcgaagcg caggacctgg aaggcggaga
     1501 tattattttc ggtcataaat tcaaaaattt cgaacttacg ctggaatgga aagtctccaa
     1561 aggcggtaac tcaggtattt tttacctggc tcaggaagtt tccacaaaag ataaagacgg
     1621 aaatgtgaag atggaaccga tttatatttc atcgcccgaa tatcaggtgc tcgacaatgc
     1681 taaccatccc gatgccaaac tcggcaaaga caacaaccgc cagtcagcat cgctctacga
     1741 tatgattccg gcagtgcctc agaaccaaaa accctttggc gaatggaata cagccaaaat
     1801 tatggtttat aaaggtaccg tggtgcatgg tcagaacgac gcaaatgtag tggaatatca
     1861 cttgtggact cctcagtgga ctgaaatgct tcaggcaagt aaattcagcc aggaaaaatg
     1921 gccgttggct tatgaattgc tcaacaactg cggcggtgcc aacagggaag gttatatcgg
     1981 acttcaggac cacggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggaa
     2101 atggtgggac actgcaattg cggctcacaa agcggccggt atgaaataca tcgtaaaacc
     2161 ctggatgcct gttccaaaga ccctggccga cctaaaagcg tattgcgatt attacaacca
     2221 ggtgggcgaa aaatgcaacg cagcaggact caggtttgga tatcacaacc acgattttga
     2281 atttgcgaag atagaaggtg aaaccatgta tgattacatg ctgaaaaata cagatcccgc
     2341 gaaagttttc ttccagatgg atgtgtactg gacagtgatg ggcaaaaaag ggccggtgga
     2401 ttatttcaac gaatatcccg gacgtttcga aatacttcac atcaaagaca acaaggagct
     2461 cggacaaagc ggtatggtgg gctttgatgc aattttcaac aacgcagata aagccggtgc
     2521 taaatatatg gtggtagagg tggaaaaata caacttcgaa ccgttgaaaa gcatcaaaat
     2581 gagttatgat tatttgaacc gaaatgcttt cttcaaaaac gattattcgg tgaaataatc
     2641 cattcactca atttcagaca gctcttcttt atcaattgcc acgacgtaaa atcgaggcaa
     2701 ttgttttttt agaaaacagt agtgaaagat aacctcaaaa gacatatatt acacctattt
     2761 aaatatattt gtaaaaaaac ggttcaaaag tccgaataaa aacttatttg actatatttg
     2821 catttaatat ccttaaaaca cgtaatttgt gagccctcaa actgttttta tcattattct
     2881 tgtcatttta acagccgatt tcgtgtcgga aagaatattg tcggctctca acgtgaaatc
     2941 ttctaaaaaa cccattcctg atctgctttc aggcatatat gaccaggata aataccaaaa
     3001 acaacagtca tatttcaggg cgaataccag attcggcctt cttacatcaa cggtgagttt
     3061 tttggtcata attttaatga ttgttttcgg cggattcggg tggctggatg gcatagtgca
     3121 gcgatggact gccaatccga tacttgtttc gctgattttc ttcggaatac tttttctggc
     3181 taacgacatc atttccactc cgtttgagat ttacgatata ttcgtgattg aggagcgttt
     3241 tggatttaat aaaacaaccc ccaaaacttt cattctcgac aaactgaaag ggtatgctct
     3301 gacagctgtt ttaggtggcg gaatcattgc gcttgtcatg tggttttatc aactgaatcc
     3361 gcaatatttc tggctgatag cctgggcatg cgtcacttca gtcagcttat ttatcagcct
     3421 gttttattcc gaactgatag tgccgctttt caacaaacaa actccgctcg aagcgggcga
     3481 actgcgtgat gctattgaaa aattttcaaa gaaagctggc ttcgaactga aaaatatctt
     3541 cgtcattgac ggttcgaaac gatccactaa ggcaaatgcc tattttaccg gtttctggaa
     3601 gaaaaaacga attgtgctct acgatacact tatcaacgat ctggataccg cagagatagt
     3661 tgctgtgctg gcgcatgagg ccggtcacag caaatacaaa cataccctca ggaacatcct
     3721 gataaatctg ccatacacgt tgctggtgtt ctttctcctc ggactgatac tccgcagcga
     3781 tgtccttgca caggcattgg gcggcaaaga ggcttcgttt catctgaata tactcgcttt
     3841 cggcgtattg tattcacccg tctccatgat gctcggactg gcaatgaatg tgctctcgcg
     3901 caaacatgaa tatcaggccg acggatatgt taaagagaat aaaatgggcg aacagctgat
     3961 ttcggcatta aaaaagattt cgtccaaatc gctttcgaac ctcactccgc atcctgctta
     4021 tgtgtttgtg aactattcac acccaacgct gctgcaacgg gttgaaaaat taaccaattc
     4081 acctgttaac caatctacta attaaccatt acaaatgata ggactcatgg gcgctatgcc
     4141 cgaagaaatg aacaggataa taggagccat cactgaaaaa agcattacag aacttggtaa
     4201 ccggttatat cacaccggaa aactattcgg aaaagaggtg gtggcggttt tctcccgatg
     4261 gggcaaagtt gctgctgcca ctacggccac caacctgatt ctggaataca aggttgacca
     4321 gataattttt accggtgtgg caggtgccat ttcggccgaa ttgaatattg gcgatgtagt
     4381 cattgcacag cggctttttc agcacgatat ggatgcacgt ccgctgatgc ggagatttga
     4441 aataccgctg accggaaaaa cctcctttga aattccggat gaaaataccc ggcagatggc
     4501 ggatgctgtt catcattttt tgcttaacaa cagggaattc cggaaacgac tggctgaaaa
     4561 tgatatcaaa aatccgaaaa tgatgcttgg agatatagcc agcggcgatt tgtttgtttc
     4621 cggaacggaa atgaaaaacg aactgatacg aaatttgccc tctgtagttt gtgtggaaat
     4681 ggagggggcg gctgtggctc aggtgtgtta cgatttcggt gtgccacttc aggtagtaag
     4741 agtgatctcg gatacagccg acgagcaggc acacacgagt gccatcggtt ttgttaatca
     4801 gcatggtgga gagtattcgc tggccattct cagagaatac ttcaaaaaaa tggattgaat
     4861 gttttttatt cctgatgttc aacgagttaa tgaaaaagta taattgtctg aataataatt
     4921 aaataactta tcaaacaaat ggaaaacaca tcattttacc tggttcctct gtcgtcattg
     4981 ttggcattgg gatttgcttt ttacttcttt cgctccatga tgaagcacag cgaaggaact
     5041 gatacgatga aaagaatagc tcagtacgta cgtgaaggag ctatgtccta cctcaagcaa
     5101 cagtacaaag tggttactat tgtattcatt gtactggcaa tcattttcgg ggtgatggcc
     5161 tatttcggat tgcaaaattc gtgggtgcct tttgccttcc tcaccggagg atttttttca
     5221 ggattagccg gattcttcgg aatgaaaacc gctacctatg cttcggcacg cacggcattt
     5281 gcagcctcga aatcgctcaa ccacggattg cggattgcgt ttcgttcggg tgcagtgatg
     5341 ggattagtgg tggtaggact tgccttgctt gacatttcgc tttggttcgt cattctcaat
     5401 tattttattc cggatgccga tccgggtcac aaactcatca tcatcaccac taccatgctt
     5461 acgtttggta tgggagcatc cacacaggcg ctctttgcac gcgttggcgg aggcatttac
     5521 accaaagcag ccgatgtggg tgccgacctg gtgggtaaag tggaagccgg aatacccgag
     5581 gacgatccgc gcaatccggc taccatagcc gataatgtgg gagataatgt aggcgatgtg
     5641 gccggcatgg gtgccgacct gtatgaatct tactgtggct cgatactcgc tacagctgct
     5701 ctgggagctt cggcatttat gacacagcct gaaatgcaac tgaaagctgt atttgcgccc
     5761 atgctgatag cggctgctgg ggtggttttg tccattctgg gaatctttat tgtgcgtaca
     5821 aaggaagatg ccgacatgaa aaaactcatg gctgccctca accgcggtgt aaataccagt
     5881 gcgctactca ttaccggtgc tacattcctg attctttacc tgcttgatat cccgaactgg
     5941 gtgggattgt ctttctctgt cattgccgga cttgtagccg gaatcatcat cggacaatca
     6001 accgaatatt atacttctca ttctttcaaa cctactcaaa aaatagcgga aagttcaggc
     6061 acaggtcctg ctacggtaat tatttcgggt atcggtaccg gaatgctttc cacggcaatt
     6121 cctgttgtga ccattgtagc tgctattgtg atttcgtttc tgagtgccat caactttgat
     6181 acagccggaa tgcttacagc cgaaaatctg agtaagggat tgtatggaat aggtatagct
     6241 gcagtaggga tgctttccac gctcggaatt accctggcta ccgacgctta tggtcctata
     6301 gccgacaatg cgggcggcaa tgcgcagatg agcgaactcg atcccgaagt gagaagaaga
     6361 accgatgcgc tggatgcgct tggaaatacc actgcagcta ccggtaaagg tttcgccatc
     6421 ggctctgctg ctcttaccgc gcttgcactg cttgcatcct acatcgaaga ggtgaaaata
     6481 ggtttggtgc gactgggcag aaccacactc caactggcta atggtgatgt gatagaaacc
     6541 accaaagcat cttttgttga ttttatgaat tattacgaag taaatctgat gaatcccaaa
     6601 gtcctcgcag gagcatttct cggttcgatg atggcgtttt tgttctgcgg tctgaccatg
     6661 aatgccgtcg ggcgtgctgc tcagaaaatg gtgaatgaag ttcgtcgtca gttccgcgaa
     6721 ataaagggaa ttctggaagg aaaagaaact cctgattatg ctcgttgtgt ggaaatttcc
     6781 accataggtg ctcagcatga aatgcttatc ccatcgttgc ttgccatcgt tgctcccacg
     6841 ctcaccggtt tgctctttgg agtggcaggt gtaatgggtt tgcttgccgg cgggcttgct
     6901 tcggggtttg tgctggctgt attcatggct aacgccggag gcgcctggga caacgccaag
     6961 aaatatgtgg aagaaggaca ttacggcggc aaaggttccg aaaatcacaa ggcttgtgtg
     7021 gtgggcgata ccgtaggtga tccgtttaag gatacttcgg gcccttcact caatatcctc
     7081 attaaactga tgagcatggt ttccatcgtg atggcaggtt tgattgtaag ctggagcctg
     7141 ctctgatgat attaacggaa ctgtaatttg taaaaaaagc ggactcttga tccgcttttt
     7201 ttatgcattt tttttattga tgctctttcc ggagcagatc attcactgtt ttcaccggat
     7261 tgaatgtttc gatgggaact tccacaaaaa cggtgttcca gtcgctcatg gctccgttcc
     7321 acagcccggg cagttcaagc gccttcagtt cgcggccgct tttggattta agcgaaataa
     7381 atccggtgtt cttgtccaca aaattcagca ggttgaactt ttctcccttg taatttttta
     7441 tgccgcacac cagatctacc ggattaaaat gggtagaatt cagcattttg gctttgtctt
     7501 ccggattatt ctgattgatc tgcgagcttt caagaatttg cggtgacact gtgccatcct
     7561 gattgaccgt gaggaacgga ccgcctccgg gttcacccac gtttttcacc atgccgcaaa
     7621 cacgaatcgg acgattcagt ttattgaaga ggtattggcg tagttcattc cctttcagcg
     7681 acctgatgtc cggatggtga ttgttgagtt tcgactcgca gaactggctg ataaccgcca
     7741 gtttagcatc ggatattcct tcattttcaa gttcatggag gtatgcaaac gtttgtttct
     7801 gcagatgaac aaggagcccg gcaatgagtt ttttgtaggt tacagttgct tccttcagcg
     7861 aatcaggcac tacattgtca atatttttga taaaaaccac atctgcttcc aggtcgttca
     7921 ggttttcgat taatgctccg tggccacccg ggcggaatac cagttcgccg cggtcgcgga
     7981 aaggctggtt gtcgctgtcg gctgcaatgg tgtcggtgga tggtttttgt tctgagaagc
     8041 taacctgata tttcaatcca tactgttttt cgtacttcgg aaggctttca agcaggtgtt
     8101 tttcaaacag cactctgtgc tcggccgaaa cggtgaaatg aatatttact tttccgtttg
     8161 atttggcgta cagggcacct tccaccagat gttcctgcag tggagttcgt ttgtcatcgg
     8221 ggtaagagtg aaatttgagt aatccttttg gaagtgatcc gtaactcagt ccgttactgt
     8281 tgagcaggtc cgaaaccaca tctttgaatt gacctgctgc catcaattct ggaattgttc
     8341 tgccttcatt ttttatgcag gcttcattca ggtcatcgta aaaagcaaag tgacttattt
     8401 cgtcgaagaa ttttttttca aaatctgaag aaggagtgtt ttcatctttt tcgagaaatg
     8461 aaaataagtc tttgaacatg cggcttgctg cgccggatgc gggcacaaac ttgagaacta
     8521 cggcgtccga ttgctgatat atttcccatt cgtttagcaa tttatgctga ttttcagcgg
     8581 ggatgacggt gatgccgttg ttgatggaag cggcgcttcg gatttcaaga aaaggaaaac
     8641 cgttaacaaa gcttagtaac tgattttcaa tttgttgctt cgttattcct ctttgtttta
     8701 agatgtattc atctgatggt agtagcatag tagtagatat ttatgagtta atggtcagtc
     8761 attcagttgc ttatcaagca gaattgcttc ctgtttcctt tttttcaaaa tttcataata
     8821 atccagcaat tcgtagattt catcgagtgt ggtcgatgaa aatgcaatta tattcggaaa
     8881 agtttcgact gcttttgagg catagttcat cgctttgtcc agatttccca taatttcata
     8941 ggctatggca atgttattat acgcgtgaaa ttttgttttg ttgttttttg ttgtttcgga
     9001 ggctttcacc cacaagtcga tggctgcctg ccattttttg taggtcagcg aatccattgc
     9061 ctgaacgaac aattgttttt ttggagtgta aaaataacgg tcttcttttt cccagcgcgg
     9121 tatcatccgg tcggcaatgt ttgagccggt gagaatgcat gcatcaacca gggcatcgta
     9181 gcgattgggc agtttgttga attgtgcatc ctcccacgaa aattcatctg agaattcctt
     9241 gaagacactt aatgaatctg ttgggtaatg aattgtccat tttgtgttga tttttacgtc
     9301 cagcgcactc tgaatgttgc ctgaattcat ataggattta tcttcggttt gaatatgatc
     9361 cagtgaaatc agtgcatctg cattgtacaa tccacacagg aatcttacgg tttgtttgtt
     9421 gagaggagcg aggcggtaat agttgtcggt ggtgttcatg tttgtttgtg acatgctcac
     9481 cgaatagaaa aaatctttgg cttcgaggtt ttcacgtaag ctggcagcac aaaaaagagc
     9541 tgcgctgtca aatttcaacg aaacattgac gggattattt ctggttgctg acgatttgat
     9601 atcagcatga cccacattgt atggttgcac gaccgaattg ttgacaataa gtacattttg
     9661 aacttcggga ttaaaagtta cttcggcagg tctgagtaca tcgagcgtgg taaacatacg
     9721 cggagtgacg catgagctta aaatcaatat cagagatatg ggtaaaatga attttaaaat
     9781 ctttgttttc atcggtaaac agatgttaga aatatacgta cagacaaagt taaaaattta
     9841 ttgataaatt atcataagca aagtgaatat tttggggcaa ttctttttct atctcactct
     9901 gaacaggtaa atggtggctt gcatgtgtaa aataggagct atcggcattt attttccggg
     9961 caatgcggat agcttcatct accgataggt gtgaaaagtg ttcttctttg cgcaatgcat
    10021 tgactacgag tattttcagc cccttcaatt tctcaaattc agtttcggga atggttttta
    10081 catctgtgag atacgcaata tcgccaatcc ggaaacctaa aatgggcaga cgggcatgca
    10141 ttatgcgtac gggagtgatt ttaactcctt ccgccccgaa cgtgcctgtg tcaatttcat
    10201 acaggtccag actcggtacg cccggatttt tgtatttgcc aaagcaataa ggcatattac
    10261 gctggatagc gttcagtacc tttcgttcgg cgtaaatttt cacttcgccc agcggacgca
    10321 catcatcgag cccgcctaca tggtcgtaat gctcgtgtgt gagcaggatg ccgtcgatgt
    10381 gaaaaatttt gttggtgata agctgctgac gcaggtcggg cccggcatca atcagaaaac
    10441 gttttccctg aacggtaatc agtgccgaac agcgcaaccg tttgtctctc ggatcggtgg
    10501 agcagcaggt tttgcagttg catcccagct gaggtacacc agttgaggtt cctgtaccca
    10561 gaaatgtgat attgctttcg taaccggctg ccgaaacagc ctgtaatttt ttatccggat
    10621 tattgtattc tgtaaaatta ttcatgtttt atcttgaaac tgttccattt caatcatttt
    10681 tcgattatct atattacact ttccttatcc aatgaagggg agttgaatgc tgaattttca
    10741 tccggattct ccactcagca ggatacaggt tgttgcacct gctgccgata ttcttgaccc
    10801 acccgatcgg taccgactca tacgtaagca gtacgtagcc tttcgcggtt tcgggcagcg
    10861 caatggcctc tttgctcaga tattgaatgg cagtagttat atctacttct gcggtaatca
    10921 ttttttttat atcaatagat tttgacaatg ccaggcttgt atgcggtatg aggtctttac
    10981 ctttctgttc tgcaagtgta atgccgtaat gcatcgtttt cagattattt ctgagcaaat
    11041 ccactttttc ggcgtgttct ttcggaatgg ccgatatcag gttgttttca aaaatcaatt
    11101 cccggttttc ggaatccagt aactggtttt tgagcaaatg aaaggaatgc tccgctttta
    11161 cgggtgattt ggcggatttc ttcctgcccg tgggagtgcg gggagccggt gaagtttttc
    11221 gaacgactga aataaaaaat ccttcaccgc gtgttttgtg tggaaaaaac cggcatcctt
    11281 tctctgtggc cgtgatttcc ggaaaatttg aaatatctgc atgcagaact tctgctccca
    11341 gttcgtcctc catccatttt acattgtact cattttcggc ttcgttgtac gtacaagtgc
    11401 tgtaaatcaa tataccgtcc gttttcagcg tatcccacac accgatcaga atatctttct
    11461 gacgggcagc acacacgact acattttgct cgctccattc gtcgatggct cctgcatctt
    11521 tacgaaacat cccttcgcct gagcagggag catctaccac cacaacgtca aaaaacgaag
    11581 gtaaacgctt aaaatcttca ggcgcattgt tgcacaccag aaaattatca tttccccatt
    11641 taatcatgtt ttccgacagt atataagcgc ggcttcttac aatctcgttg cttaccagaa
    11701 aacctttttt attcagaaac ctggagatga gtgttgattt tccgcccggt gcagcgctca
    11761 ggtcgagcac tgtgctgccg ggtgatgcaa attgttcgag tacctgctgc agaaacatcg
    11821 aagaagcttc ctgcacgtag tatgcaccgg catgaaatag cggatcggct gtaaacaagg
    11881 gtctctcgtc aaggtaatat ccggagtcgc accaggctac tttgcaggct gaaggtatga
    11941 gctgcaattt gtcgttcacc cgcacactca cgggcgatgg ttcgttgagc gaatctgcga
    12001 aagacgtata tttgtctttc agcaggcttt tcatggcagc ggtaaatgga gcgggtaaat
    12061 tcatatttga gtgcaaatat acttattttc cgttcggata aagcgcttgc agcaaatttt
    12121 atttgaattc ctgctttcaa aattcaataa aataagctaa tattgcaaac aaattcagac
    12181 attcaaatta attgagaaat actatgagaa ttcgatcctt attcatattt ctgactaccg
    12241 gaatacttgt tttttcttcc tgcagtaaag aatacatcac ggattacacc tctttagtcg
    12301 atccgtttat cggcaccggc ggtcacggtc atacatttcc gggagccact tatccttacg
    12361 gaatggttca gctgagtccc gatacacgta tttttgaatg ggacgcctgc tccggatatc
    12421 acttcaccga tactacaatc aatggatttt cacatacaca tcttagcgga acaggtatag
    12481 gtgactatac cgatgtgctt gtgatgcctg tagtaggaga gcccgatatc cgcaaagcaa
    12541 ctcctgaaag tcagcaaact tcttttgcat cgccgttcag aaaagaaacc gaaaaagctg
    12601 taccgggata ttatacggca gaactggacc gctacaaggt gaaagccgag ttaacagcca
    12661 cacagcgtgc cggatttcat aaatacacat atccactgag cgataaagcc ggattgctga
    12721 ttgacctgga ttataccaat cagaatcatc ctaccgacag tgtaattatc gaaatagtca
    12781 gcgataccga aattcgcgga ctgaaaaaaa gtcggggatg ggctcgtcag caatggattt
    12841 atttctacgc cgtcacttcc aaaccgttta cttctgccac tttttacaaa aatgattcga
    12901 tagtgcctga ctcgatgctg ctgaacggaa aaagcggaaa aattttcctg tcatttaaca
    12961 ccaaagacag agaagaagta atgctcaaag tcggaatttc ggcagtggac atagatggcg
    13021 ccaggaaaaa tctggagcag gagatacctg cttgggactt tgaaggtgtg agaatcaata
    13081 caagaaaagt gtggaatgac ctgttggcaa aagttgatgt ggaatcgaaa aatgaaacag
    13141 ataaacgaat attttataca gcactttacc atacagccat tgctccgaat gtattctccg
    13201 atgtggatgg ccgttaccgt ggaatggata agcagatcca ccagtcgggg gagaatgtgt
    13261 acaccgtttt ttctacgtgg gataccatgc gggcacttca tccgctgctc accattatca
    13321 atccggaact aaaccagaat ttcattcgca ccttgctttc caaatacaaa gagggcggta
    13381 ttctgcccat gtgggaactt gcagctaact acaccgcaac catgatagga tacaatgctg
    13441 tgccaattat tgtggatgca tacatgaaag gcgaccggaa ttttgatctt gatctggcgt
    13501 atgaagctgt ccttaaatct tcggcatacg atactaccgg cattaaagat actcctctct
    13561 atatcaacgc tttgcagccc atttcaaaga aatttaaaaa tgaaacaggc tacattccct
    13621 tcgataaaga aatagaaagt gtggcaaaag gtctggaata tgcttacgac gactggtgca
    13681 tactccagat ggcccgggac cggaaagata cagccaacat aaccaaatac gaaaaactgg
    13741 caacgaacta caggaattat tttgatgccg gcaccggatt tatgcgcggc aaggactcga
    13801 aaggaaactg gcggtcacca ttcaacccca gaagctccgt tcaccgggat gatgattact
    13861 gcgaaggaac tgcctggcaa tggctttggt tcgtgccgca tcaggtggat agtttggtac
    13921 aattattggg tggaaaagaa aaattcgtgg ccaagctgga cggcttgttt accgccgact
    13981 ccaggataga aggcaaccag acttcggtag atatttcagg gcttataggg cagtatgctc
    14041 atggcaatga accgagtcat catattattc atttatacaa ctatgtggat atgccttata
    14101 aaacgcagga attgattgat acggtgcttt attcgcagta ttccgacaaa cccgacggac
    14161 tttcgggcaa cgaagactgc ggacaaatgt cggcctggta tgtgctcaac tccatgggat
    14221 tctatcagct atgccccgga aatccgactt attccatcgg aaggcctgtt tttgataacg
    14281 taagaattac tttgaataac ggcaggtatt ttgaaataaa aacctataac aattcaagaa
    14341 aaaataaata catcaaaagc ataaaactga acaataagaa actcgaaaaa ccatttttta
    14401 cccacgcaga gctgatggcg ggcggtaaac tggaaattgt aatgaccgac aaagcgaaaa
    14461 aataaccaac tgcagatttt tttttgaaaa aaatagaatg atgaggaatt taattttatt
    14521 cccgtttttg ttgttagccg tgtttcttcg ggggcaggag gtgttccaaa cccgtatttt
    14581 acatgaaaat gtgaaaacac tgcaggtgaa agtgaacgat aatccgttga ctttgcctgt
    14641 tattacactg aacgggaatg atgtactcga aataagtttt gacgagatgt cgcatgaaaa
    14701 acgttcattc tcgtacgaaa taaaacactg caatgccgac tggacaatct catcactttc
    14761 ctcatccgaa tacttgcagg gattctcacg cggatacatt gacgactacg ctttgtctgt
    14821 caacaccacg tttctttaca ctaattacag gttcatcctg cccaataacg atgtatcttt
    14881 tgccgtttcg ggcaattatc tgatcaatat ttacgaagac aatcaaacgg ataatcccgt
    14941 ggctcatgtc tgtttttcgg tagtggagcc gcaggtggaa ataagtgctg ctgtgcgtgg
    15001 aaataccgat accgagttga ataaatcgat gcagcaactt gattttgatg ttgttatgcg
    15061 aaattataaa attcaggatg ctcagactga attgaaagta gtggtaaggc aaaataaccg
    15121 cacagacaat caggttaccg gtctgaaacc tactttttac tcagccgata aactcagttt
    15181 tgtcaataac cgtcagctta ttttcgaagg tggaaatgag tttcatcgtt tcgacttttc
    15241 atccatctac aattacgatg agcgcattgc tcaaataaaa tttgacagac cgtattatca
    15301 ggtttatctg accgaaaaca gcattcaggc agacgatgcc tatatgcagg attttgatgt
    15361 gaacggacga ttcgtggtca actatcaaaa cgcgatagaa agcccggacg tggaagccga
    15421 ttatatgttt gtgaattttt atctgcccgt aaaccgtccg tttattcagg gaaatatctt
    15481 tctgggtgga aactggaact acaaccggtt gaacgcactc tcccgaatgg attacgaccc
    15541 ctccaatggc ttgtattaca aaacgttgct tctcaaacag ggcggataca actatcagta
    15601 tttgtttctt cgtgaaggtg agaccagggc cagtacgaaa cctgtagacg gcagtcactg
    15661 gcaaacccag aacgaatatg ccatctacat ctatcaccgg ccgtggggtg ggagatatga
    15721 caaactggtg ggtgtaaaga ttctttagtg tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnngtgttc atttgcttaa ttaccaataa ctcaataacg ttatatgatt
    15901 gtttataaat tcggaggagc gtcagtaaag tcgtctgacg gtataaaaaa cattgtacaa
    15961 attgtatcgt cggtacagga cgacctgttt atcgtggttt cggctatggg aaaaacaacc
    16021 aacgctatgg aagtagtgct tgattttttt atgaaagccg aaagggaagc agcactcgta
    16081 aaactgaccg aagtggaatc gtatcatcag cagattattt cggaattgtt tgatgataaa
    16141 accgtaggtg aaaaggctgt agaaccgctt tttgaccaac tgaagcattt cctgaacgaa
    16201 gctgtgggaa atgattacga ccgctggtat gatcagcttg tttcatacgg cgaggtgatt
    16261 tctacggcca ttgtttccgc ttttctgcag gaatcgggtg ttgaaaacaa atggctggac
    16321 atgcgtgagc tactggtgac cgacagtaat ttccgcgaag cgaatgtaaa ctacctggaa
    16381 tctgaaaaga gactgaaagc agctgtggat ttcagcaaac acaaagtgta tatcggtcag
    16441 ggatttatag gtaccactat gaaaggactg cctaccacac tcggtcgtga aggttcggat
    16501 tatacggctg ctgttgtggg caatctgctg gatgcttcgg gagtaaccat ctggaaagat
    16561 gttcccggta ttttgaatgc cgacccgcgg atttttccca atacggtgct tattcccgaa
    16621 ttaacctaca tggatgctgt ggagctggcg ttcagcgggg cgcagattat ccacccgaaa
    16681 acaatcaaac cgcttgaaaa caagaaaata cctctttatg tgcgtccgtt tgggagtccg
    16741 caggaagccg gctcggtcat caacaattca gcgcctccca ttcaggtacc cgtactgatt
    16801 ctgaaaaaaa atcaggtact ggttacgctg cggccgctcg atttttcgtt tattctggaa
    16861 gaaagtctga cctggatttt cacggttgta gaaaaacacc ggctgaaagt ggctttgatt
    16921 cagagttcgg cggtgagcat ttccatttgt gtggacaaca gccgttacct gcagagtgcg
    16981 ctggacgaac ttgccaacga ttttaaagtc agctacaaca cggggctgga attgctgacc
    17041 attcggggaa ctaatccgga cattatccgc cggactaccg aaggccgcga aattctcgta
    17101 caacagaaca cacgcaggat cggacgattt gtgatgaagg aaaaaccgga aaacggagga
    17161 tagcctctgt tggctgtcag atagtttata agaaaaggca tcggaagatg ccttttattg
    17221 ttctattgcc tttgaattgt tattggatcc gatgcctttt cagtcgttga aaataaacca
    17281 tatgttggta atagaaggac cggatttgtt acaaaaccca catgatttta tcatttcttc
    17341 atttggtttt tatgattttg caaaggattt ttacaatttt gtcatatgtt tttgctattt
    17401 tgtaaagagt ttttggttcg tggttaaaga attttggttt gtggttaaag aattttggtt
    17461 cgtggtaaaa gatttttggt tcgaggtata agattttcag ttcgtggtaa aagattttcg
    17521 gtttgtggtt aaagattttt ggttcgtggt caagagtttt cttgattttg taaagcgttt
    17581 ttacgatgat gtaacgtgtt tttatcaatg tgttggaggg tttcacgggt tgtaaggata
    17641 tttaaatcat aggtattaac acagaaaagg tatcttccga tgcctttttt gttctataat
    17701 aggaaaagaa tacactatat ttttttaatc attacttcct aaaaaataaa tagaatatag
    17761 tggaagcata ttcttatcct catctgtcat ttttgcataa aattcttgcc aaagagcaat
    17821 aaaattgtca atatccaata attttatatg tctgtgactt tctctagctg atcgttctgc
    17881 ttcagaagtg aacgaacctg atgttacaaa caaaccaacg tcaccatctt tgtttaataa
    17941 gccagttaaa cttcgaatat catcaacggg aactgaagag tctggacgat gtttaacttg
    18001 tacttttagt cttggactcg ttgcacctaa gggatcatta tatgctataa tatcaagtcc
    18061 gccatctttg ccttttggtg atataaaagg tgtgtgatat ttcatcgcac gaagcaaagc
    18121 cgcaaccata tcctgaaatt cgtatgcatt cttactccta atgaaatctt ttattccagc
    18181 tattgccctt tcttccaata aatctaaatt agccttttgt gtttgagttt tattttcttc
    18241 aagttcagtt tgttcatttt cttgatcttt actttttata gatttgtttc cttttttatc
    18301 agctgcccaa gatctataaa attgagatgc agtctcaagt agtttaacag gcccaagaga
    18361 aattgctttt tctccttcgt ctgttaaata ccaaattcct ttattttttc ttaggtaacc
    18421 tgctttgata gcatcgattg tataaaaatg tagaaaagat tcccatctta catatccagt
    18481 tttttcataa acttctaact cccagtcgct taattcaaca gaatctctga ttctttcaat
    18541 tacttgtttt ccaggcaatt gtcctcctgc ttccttgagg atattaaaag ttgcaaatat
    18601 agtttttgct gcagattgtt ttgaaggagc aagtttttga aggttattat tcatgatttt
    18661 aacaaataaa atgatggttt attattagga gtataattta gatatttttg aattcgatga
    18721 tttatgtgtt ttattttaat tgtatttgag gaaatatttg agatttttac aattttcttt
    18781 ctttctgatt ctcaaaaatc tccagttgca gtttcagata ccccaaaagt ccgatagcca
    18841 gaatgatgga gccgtatgaa aattcgcgaa acagactcgt ttttacaata aatcccagca
    18901 atccggcaat aagaaagaca aacatggata tcagcgtaaa caggatggta gttttagccc
    18961 ttttgggatt gctccagatt cgtttttcgt atttcaagga agttctggac gaatcctgaa
    19021 gcgcagcaaa actcaaagtc aaacctaaga atatcagggc attattgtaa gtgccgaaca
    19081 gatattccgg attagccgac aagtaatcaa aacctttgag tatgggtttg acaatcagat
    19141 acaatccaat cagcaataat ggatactgga tataactcag cgtgtggaaa aattgtttca
    19201 gatttttcat gcgtgttttt gataagaaag caaaaataga actacaaata tactacttcc
    19261 tgctctgaaa aaaaaaacag ccttcaattt tcatcaaaag ctgttctcaa ttatcaaaag
    19321 aaatcatcta aatcaaaaga atcacagttc agacgatttt atccattcca caatccattt
    19381 tcccacttcg cttgttttgt aggctttggc tccatcggca atgtcttcag taacgatacc
    19441 tgcgtccatg ctggcggtta cggcatcgcg aatcagtttg ccttcttcca tcataccgaa
    19501 tgcgtattca aacatcaatg cggctgaaag tatggtagct agtggattgg caatgtcttt
    19561 tccggcagcc tgcggatagg aaccgtggat aggctcaaaa accgatgtgt gcaagccgat
    19621 agatgcggat ggaagcattc ccagcgaacc ggtaattaca gatgcttcat cggtcaggat
    19681 atcgcc
//
LOCUS       DAORKP010000022        19343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_137_length_19343_cov_9.091663, whole genome shotgun sequence.
ACCESSION   DAORKP010000022 DAORKP010000000
VERSION     DAORKP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_19343_cov_9.091663"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..927
                     /locus_tag="PLN75_02415"
     CDS             7..927
                     /locus_tag="PLN75_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRG03002.1"
                     /translation="MKVLIATDKPFAKVAVDGIRNEIEGVGFELALLEKYTEKQQLLD
                     AIADADAVIVRSDIVDEEVINAGKKLKIVVRAGAGYDNVDLEAATRAGVCVMNTPGQN
                     SNAVAELVFGLLVYAVRNFYNGTSGTELMGKKLGIHAYGNVGRNVARIAKGFGMEVLA
                     YDAYCRAEDIKQEGVNPTCSVKTLYQGCEIVSVHIPLTSETRQIINKELLELLPANAV
                     MVNTARKEVVNEADLLQHMIDNPKFKYVTDVAPDNQAEIKEKAGDRYFATAKKMGAQT
                     AEANVNAGIAAAHQIVDFLKNGNQKYRVNN"
     gene            complement(1061..1690)
                     /locus_tag="PLN75_02420"
     CDS             complement(1061..1690)
                     /locus_tag="PLN75_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRG03003.1"
                     /translation="MTQTSPLVITISRELGSGGAYIGHKLAEKLNMYYADHEIITRTA
                     EKLSVFVEDVAPHEEKITSFWQNFWEKTSLNEFYNEAVGSYIPTTSKIFETESEVIQQ
                     IAREHSAVIIGRGGFHVLRDRPNVVSIYLHGSIQARAKRLMEARHITELEALTQIAAG
                     DKQRALYIQKFTKKDWADVRNFDLSIDTGKIGFDNATEIILKYIESRQI"
     gene            complement(2070..2912)
                     /gene="nadC"
                     /locus_tag="PLN75_02425"
     CDS             complement(2070..2912)
                     /gene="nadC"
                     /locus_tag="PLN75_02425"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRG03004.1"
                     /translation="MNKDFINMIPAWFAEDIGDGDHTTLSSIPADARGKSQLIIKEDG
                     IICGVEVAREIFRAFDPELKMTVFIHDGETVKYGDIAFVVEGRIQSLLQTERLMLNIM
                     QRLSGVATRTREYMKELEGTNTKVLDTRKTTPGLRMLEKYAVKMGGGENHRIGLYDMI
                     LLKDNHVDFAGGITQAISGAQKYLKEKNKNLKIEIEVRNFDELQQVLDFGGVDRIMLD
                     NFSVADTRKAVEIIGHRYETESSGGITLQNLRQYAECGVDFISVGALTHSVKSLDMSF
                     KALD"
     gene            complement(2909..3304)
                     /locus_tag="PLN75_02430"
     CDS             complement(2909..3304)
                     /locus_tag="PLN75_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRG03005.1"
                     /translation="MKTIQILTFIAFFTLLTQAVPAQTTGFPAEIVTALNTGDAAKLT
                     SYLNDNVELVVSSKNDVYSKQQAVGIITDFFRKNKVNSFQLLHNGNKEAASFIIGSLK
                     TSTGNYRVYVLTRKISGKQLIQQLRIESE"
     gene            3491..3964
                     /gene="rlmH"
                     /locus_tag="PLN75_02435"
     CDS             3491..3964
                     /gene="rlmH"
                     /locus_tag="PLN75_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRG03006.1"
                     /translation="MKIKLIMVGKTTDKILAGLIDEYANRLKHYIMFEQIIIPELKNA
                     RSLTFEQQKEKEGLAILAKLDNSDEVILLDERGKQFSSVDFAALISNKMVFSNKSMVF
                     VVGGPYGFSPDVYQRADSKLSLSKMTFSHQMIRLLFAEQLYRAMTIIKGEPYHHE"
     gene            3957..7583
                     /locus_tag="PLN75_02440"
     CDS             3957..7583
                     /locus_tag="PLN75_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRG03007.1"
                     /translation="MSRTKNFFDRMHLSHKILLLVMLLLVTGGFVFQQIYKNSAKHAF
                     SIRNFTGELHQREYKADLKIKEIEGLLRKENTDRLPYISFEKLPSVYLVYKNDQLIFW
                     SDNQTEPQNLKNEKWHYALLSNVHAIARSHQTGAYNIVAYIPVKYNYQYENRVLKNVF
                     SDGFRMNKNIGIVSNNPASSGAVFSRQSDYLFTLEAPEKPLYNEAWAITSMIMFLIAF
                     LILFYLFARLPLLYGLSYISWKNFLVAGLLMAVFVFTCLNFNFPASFFLNKVFTPFHY
                     ASNIFLKTLTHLSFLSLFLFSLVYLYTYHVKAELTEDRNNKIKQIALLLIPGSFFMLI
                     FVFLKSVVFNSSEDVNILKLEDITLVAVWNHLLFLLWGISYMLLHARSFRILLRKNNI
                     ITLARLDFFVSVFVCLAGYLMFDKYGLLFVTAYIVLTAVLYLPYVFTKLMGPNLTTVV
                     WLMFFTLFITWSSVTMNQDKKYAKYRTLAENHYLNEGTDEDRFAIALLDDLNYSIRND
                     KRIKHLVQFPDSISVANEYLNKKYLRGFWNKYDMRLFATLPDSELDMQYNQQISNWGR
                     RVKNTNFYSMGNPDSDMSFLGVFSVVKNDRASTDFYMEFYPKKYFKSYSFPDLLLDTP
                     PGIQSKFNLSTARYTFRELVNSAGSFQYPGDAGWVEKRKENYFSQVYAGYRHYIYVPN
                     VYNYFILSEENATGTKFIIYFLYTFLLYLFISFFTLWIYRLIHRQTRIRFNFSSKFLY
                     SFTILLGLSFLSIFYVSVNYMQQKYVDDQKQKLEETKKYIQSALQEKYSWRESLDSTL
                     TSDLNFDLQDLSYTYQTDIHVYDNKGRLIASSQMPLFSRELISRQISPLPFFSNVLNL
                     NQYEHIGNLEYLIAYTDFYNIDYLPIGYIAVPQFLTNDRVKEDLESFLSVIINIYLII
                     IVLFIALSLFIGRQLSSPLLMLQDSLKKIKLGQKMQKIDYKPKDEIGQLVEQYNRTVE
                     ELEKSAQLLARSERESAWKTMARQVAHEINNPLTPMKLTIQQLQRRKAMNDEHFDEYF
                     EKSSSTLIEQIENLSKIAGTFSTFARLPDAKFDKVDVARKLSSVVILFSNNNENMNIT
                     YEGPDENIFVWADQEQMIQVFNNLLKNAIQSIPATRRGEIGVNLELTEKWIHIYIKDN
                     GKGIADEIKDKLFTPNFTTKTTGMGLGLTITQSIVMMFGGGITFETKEDEGTTFKIAL
                     PRIY"
     gene            complement(7591..8430)
                     /gene="prmA"
                     /locus_tag="PLN75_02445"
     CDS             complement(7591..8430)
                     /gene="prmA"
                     /locus_tag="PLN75_02445"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRG03008.1"
                     /translation="MRYLAIHLRFDNKEDFVADVLADYLGKIGFESFVTTDEGLDAYI
                     PEKNFSEQEFETALKRFPLDVAMSYVAEEMEDKNWNEEWEKHFFEPITIDNECIIHSS
                     FHKDIPKLKYNIIIDPKMAFGTGHHETTSLMVSEILKMDLTGKSVLDMGCGTSVLAIL
                     ASLKGAESITAIDIDEWCVRNSLENIALNDVNNIRVLLGDASLLTGQHFDVIIANINR
                     NILLNDMEKYSACLPAGGELFMSGFYTEDLPVIESKAAAYGLKTDYFNRKNNWTVVKM
                     TKH"
     gene            8613..10037
                     /locus_tag="PLN75_02450"
     CDS             8613..10037
                     /locus_tag="PLN75_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03009.1"
                     /translation="MKRFLILINLIIFGLSLGFAQSGKIIDDIYVTPNDAQMVNEVKS
                     AVKSKAKKPETPQSTIYRNGAREIVFIDQYGNRTTAVSDTVYIVDGEMIADTLAADSI
                     ADEEGYYLNGFSGSKSDLEYAERIRRFHNPKYTITIADPGYNDIYFLDENYWNVYIDG
                     MYATITPTWTNPYWFNYQYSPYSYNSWAWRHNWGYPYSGWGGGWYDPWYSGYYGWGGY
                     YDPWYSGMYGWGYPYGGYYGWGGYGGWGYPYYGGYYGWGGYGGWGYPYYGGGWYDTTN
                     QTSRRNYAGGRSAFDSNTSSRRSSVINSAAGTSRSGYTSVSRQPSATTSQRTAATTSR
                     TTRSSYSPGVDTRMRTGSVRSTQEWSNVRSNNTRVYSSPEAGERSSSVINRTNPATRS
                     SSVNSSSGRTYESRSSSSAPSRTYDSNSGSSRSSTPTYTPSRSSDSGSSRSSGSYSSG
                     SSGSYSSGSSGGGASRSSGGGGRR"
     gene            10122..11636
                     /locus_tag="PLN75_02455"
     CDS             10122..11636
                     /locus_tag="PLN75_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03010.1"
                     /translation="MKKYIFAAISLSLSLLAGAQTEYDALRLSQSELTGTARYMSLGG
                     AMGALGGDASAMKDNPAALGVYRSTEITGTMNLGLNNNQPITWQGRTTNQESRSDITF
                     NNLSYVMTMPVSEGKSLVGSNFSFSFQKLNDFNKSFTINGNKSPRSFTDFLAGFSSPN
                     PPITGDVSYDNYDMPWLTVLGFDGYLIDIDGQYFKSVLNQGELVTPSYLFDQYGALNE
                     YSFGWGGNFNDNLFIGANLNIRSLDYTLSSTLSESFAEGGGFDLVNKLTQSGMGLNAK
                     LGAIYLPNNSLRLGIALHTPSISYFTEESYADLNSGLIPNDEKNPAQTPVNSQSFNLW
                     SPMQLQASAALLFGKTGFISAEYNFVNYRGARFNGNSNSVQNFGDINMAMKDVLNNVH
                     VLKAGAELKVSPSFSLRAGYAMMTPAVNTGYNDGKLLVRNAVNTNTEYFDQKYNTTYM
                     TFGLGYRQASWFIDLAYALRNQKEDFYPYQDKALPPAVIENKTNNIALTLGLRM"
     gene            11836..13410
                     /locus_tag="PLN75_02460"
     CDS             11836..13410
                     /locus_tag="PLN75_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRG03011.1"
                     /translation="MSLLSEINRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFEYDDYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVVDAAKGVETQTRKLMNVCRMRNTPIIVFVNKMDREGRDPFDLMDELEAELG
                     INVRPLSWPISMGATFKGVYNFFEQNMQLYTPNKQFISESVEIDVNSSDLEKKVGEKD
                     AAKLREDMELINGVYADFDKEMYLKGNLAPIFFGSALNNFGVKELLDCFVRIAPSPRP
                     VQTTERIVEPNEEAFSGFIFKIHANMDPNHRSCIAFVKICSGKFLRNVNYKHIRNGKM
                     MRFSSPTAFMAQKKETVDEAYAGDIVGLPDTGNFKIGDTLTSGEDLHFKGIPSFSPEM
                     FMYLENADPMKSKQLEKGINQLMDEGVAQLFVNQYNNRKIVGTVGQLQFEVIQYRLLH
                     EYGAQCRWEPVSLYKACWIESNNAVELENFKKRKMQYMAKDKEGRDVFLADSNYVLQM
                     AQNDFKDIKFHFTSEF"
     gene            13422..14060
                     /locus_tag="PLN75_02465"
     CDS             13422..14060
                     /locus_tag="PLN75_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03012.1"
                     /translation="MRKSIILAILLSVTLSFFAQEKRDKPIPVDLLFGDKRVNLMFSM
                     NKTIDGNFRFINSTSAAAYYDYLKGKTEMVSTSSIVYQFHKNIGISGGMQYHFAKGFI
                     PNIAMHFSHGNPTWLIALTPYYNFMPWNSLEAAAIAEFKPALADNLRLFTRLQGFYSY
                     DLKKDERERGIINFRLGLTMKKYTVGFGGVIDYYRPTANEIQNYGGFVRVEI"
     gene            complement(14214..15275)
                     /locus_tag="PLN75_02470"
     CDS             complement(14214..15275)
                     /locus_tag="PLN75_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG03013.1"
                     /translation="MNKEIIPPVDRSLLKKELTEIRYVRPTNKGKNDIYIVTAHDSPN
                     VMREIGRLRELSFRSWGGGTGNELDLDDFDFLKKPFKQLIVWDPQAEEIVGGYRFLSG
                     RDVELLPDGQPNFVMSHLFTFSDRFMKDFMPFCIELGRAFVQPDYQTAKMGVKSLFAL
                     DNLWDGLGALIHTEKKSKYFVGKVNIYNTYPQLARELLYEYMFLHCPDPDNLIYPKIP
                     VAVSEESKKIAQVVLTEKNAEGNYKALQKTIRHIGTTVPPMFNAYIGLTDTLRMFGSM
                     IDPDFGGTYESAIMLTMDDLTESKRQRYIEPYVQFLKQKINEKRAARRAAQILKEKEE
                     VPSKIKQATKDYLEKRKKK"
     gene            15555..16328
                     /locus_tag="PLN75_02475"
     CDS             15555..16328
                     /locus_tag="PLN75_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRG03014.1"
                     /translation="MKKILITAVVIFSCMAVMNAQKNELFLKKEFVFKGDTLKYRVLF
                     PENYDRTRQYPLVVFLHGSGERGNDNEKQLVHGASLFTDSENRKDYPSIVIFPQCPQE
                     DFWVEREKIDFENIEFPKKPKITKSLYLVKKLLDSYKKTEAVDDHKIYVMGLSMGGMG
                     TFDLICRYPKYFAAAIPICGGVNTERLKKVKKMPIRIFHGGADPVVSREFSRNAHIEL
                     KVQGSVKVEYIEFPGIGHDSWTKAFQYPDFLSWLYYQSR"
     gene            16482..16718
                     /locus_tag="PLN75_02480"
     CDS             16482..16718
                     /locus_tag="PLN75_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRG03015.1"
                     /translation="MAKLKGAVIVNVERCKGCNLCVVACPQDVLLLNKEANSKGYNYS
                     YMENPEECTGCTTCALVCPDACITVYRIKVEEKN"
     gene            16747..17826
                     /locus_tag="PLN75_02485"
     CDS             16747..17826
                     /locus_tag="PLN75_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRG03016.1"
                     /translation="MEEVTLMKGNEAVAHAAVRAGCDGYFGYPITPQSEIMETLMEMK
                     PWETTGMVVLQAESETASINMVYGAAGCGKRTMTSSSSPGVSLMLEGFSYIAGAELPC
                     VVVDVMRGGPGLGTIQPSQADYYQIVKGGGHGDYKVITLAPATVQEMADFTVLAFDLA
                     FKYRVMCVVLADGVVGQMMEKVVLPPFRPRWTEEEIRRMHPWATLGRTPDRKPNVITS
                     LALQAEEMEEINLRLQSRYREIEKNEVRYEEVMCEDAEYLIVGFGISARIGQKAAEMA
                     REEGLKVGLLRPITLFPFPTNEIKRLAEKVKGVLTVELSAGQMVDDVRLAVNGKVPVE
                     FFGKMGGIVPSPDEVLHALKEKVIK"
     gene            18018..18776
                     /locus_tag="PLN75_02490"
     CDS             18018..18776
                     /locus_tag="PLN75_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRG03017.1"
                     /translation="MTTQIIEPQNLVYKKPSLLNDSNMHYCPGCSHGVVHKIIAELIE
                     EMGIADKAIGVAPVGCAVFAYKYIDIDWQEAAHGRAPALATAINRLVPSSMVFTYQGD
                     GDLAAIGTAETIHACNRGENITIVFINNGIYGMTGGQMAPTTLVGMKTATSPFGRDID
                     LNGHPLNITELLAKLPGTCYVTRQAVHKVGDVRKTKKALQKAFQNTLDKKGTSVVEVV
                     STCNSGWKMTPEASNDWMVENMFPAYPLGDLKNL"
     gene            18806..>19343
                     /locus_tag="PLN75_02495"
     CDS             18806..>19343
                     /locus_tag="PLN75_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRG03018.1"
                     /translation="MKEEILISGFGGQGVLSMGKILVYSGLMQGQEVSWLPSYGPEQR
                     GGTANVTVILSDEKISSPILNKYDTVIVLNQPSLDKFESRVKPGGTLIFDSNGFKRFP
                     ARTDINIYKVDATEYAYKHDMVKTLNMMILGGFLKIRPIVKVENVLKGLKKSLPERHH
                     HLIPMNEKSILKGMDIVEK"
BASE COUNT         5668 a         3938 c         4215 g         5522 t
ORIGIN      
        1 atttcaatga aagtacttat tgcaaccgat aaaccatttg ccaaagtagc ggtggatggt
       61 attcgcaacg agatagaagg agtaggcttc gaactggctc tgctcgaaaa atacaccgaa
      121 aaacagcagc tgctggatgc cattgccgat gccgacgcag taattgttcg cagcgatatt
      181 gtggacgagg aagtgattaa tgccggaaaa aaactaaaaa ttgtagttcg cgcaggtgcc
      241 ggatacgaca atgtggatct ggaagcagct acccgtgccg gagtatgcgt catgaatact
      301 cccggtcaga actcaaatgc cgtggcagaa ctcgttttcg gactgttggt ttacgccgtg
      361 cgtaattttt acaacggaac ttccggcact gaactgatgg gcaaaaagct gggaattcat
      421 gcctacggaa atgtgggacg gaatgttgcc cgcatcgcca aagggtttgg aatggaagtt
      481 ttggcttacg atgcctattg ccgtgccgag gatatcaaac aggaaggagt taatcccacc
      541 tgctcggtaa aaacactgta tcagggttgc gaaattgtgt cggttcacat tcccttaact
      601 tccgaaacgc gacagattat taacaaggaa ttgctggaat tgcttcctgc aaatgcggta
      661 atggtgaata cagcccgcaa agaagtggta aacgaagcag atctgttgca gcacatgatt
      721 gacaatccga aattcaagta tgtaacagat gtagcccccg acaaccaggc agaaatcaag
      781 gaaaaagcag gcgaccgata ctttgccact gctaaaaaaa tgggcgcaca aaccgccgaa
      841 gccaatgtga atgcaggaat tgctgctgct catcagatag ttgacttcct gaaaaatggt
      901 aaccagaaat acagggtaaa caactgatta acttactgaa taatagaaaa tgaaaaggat
      961 tgcaggtgaa aacaggtaat cctttttgtt ttcaataaaa aaaccgtttt cctgtttttt
     1021 ggaaaacggt tttttatgac agattaaaaa aaacattcaa tcagatttga cgagactcga
     1081 tatacttcag aatgatctcc gttgcgttgt cgaaaccgat tttcccggtg tcgattgaca
     1141 aatcaaaatt acgtacatca gcccagtctt ttttggtgaa tttctgaata taaagtgcac
     1201 gctgtttatc gcccgcagca atctgtgtca gagcttcgag ttcggtgatg tggcgtgctt
     1261 ccatcagcct ttttgcccgt gcctggatgc tgccgtgcag ataaatgctc actacgttcg
     1321 gtcggtcgcg taaaacgtga aatccgccac gcccgataat tacggcggag tgttcgcggg
     1381 ctatttgctg aatgacctcc gattcggttt cgaaaatttt tgaagtcgtg gggatgtaac
     1441 ttcccaccgc ttcgttgtaa aattcgttca aactcgtttt ttcccagaaa ttttgccaga
     1501 aagaagtgat tttttcttca tgaggagcta cgtcttctac gaaaaccgag agcttttcgg
     1561 cggttcgggt aatgatctca tggtctgcat aatacatgtt cagcttttca gccagtttat
     1621 gaccgatata ggcaccgcca ctgcctaatt cgcgactgat ggtaattact aatggagaag
     1681 tttgtgtcat tgttttacct ccgtttcttg gtttttataa tttgtaattg taggaatgtt
     1741 atctctgaag tatgataaaa ttattgctgc gaattaaatt aatggttgaa tcagattttt
     1801 tttaatgtaa acgtcagatt tttaaaaaat atttttctgt tgaaaccggg taagagtata
     1861 agggtagttg attgatattc aacctgttgc gggtaacatt ggtgttcgga gtattcttca
     1921 gcagaaaata ctccgaaaga tcacgataaa gataaaaaag aattttatac agaacaaaat
     1981 attcacatag aatatgttat tttttcttcc ataaaaaaaa accgtcctcc tgataagaaa
     2041 acggtttaat gtggtaaaaa gttttgcgtt tagtccaaag ctttgaaact catgtctaaa
     2101 cttttaaccg aatgagtaag agctcctacc gatatgaaat caacgccgca ttcggcgtat
     2161 tgacgcaggt tctggagtgt aatgccgccc gatgattctg tttcgtacct gtggccgatg
     2221 atttctacag cttttcgggt gtcagctaca ctgaaattgt cgagcataat gcggtcaact
     2281 cctccgaaat caagcacttg ctgcagttcg tcgaagtttc ggacttctat ctctattttc
     2341 agatttttgt ttttttcctt caggtatttt tgtgctcccg aaatagcctg ggtgatgcct
     2401 cctgcaaaat ctacgtggtt gtctttgagt agaatcatat catacaatcc gatgcggtgg
     2461 ttttctccgc cgcccatttt tacggcgtat ttctcgagca tccggagtcc cggcgtggtt
     2521 ttgcgggtgt caagcacttt ggtgttggtt ccttccagct ctttcatgta ttcacgggta
     2581 cgggtagcta cgccactgag ccgctgcatg atgttgagca tgagcctttc ggtctggagc
     2641 agcgattgta tcctgccttc caccacaaat gcaatatcgc cgtattttac ggtttcaccg
     2701 tcgtgaataa acacggtcat tttcagttcg ggatcaaaag ccctgaatat ctcacgggca
     2761 acttccacac cgcagatgat gccgtcttcc ttaatgatta actgtgattt tccacgggca
     2821 tcggccggga tgcttgaaag cgtggtgtga tcgccatcgc ctatgtcctc ggcaaaccat
     2881 gccgggatca tgttgataaa gtctttattc attcagattc aattcgtaat tgctgtatta
     2941 gttgttttcc gcttattttt cgggtgagca catatacgcg gtagttgccg gtagatgttt
     3001 tgaggctgcc tatgatgaaa cttgcagctt ctttattgcc gttgtggagc aactgaaaac
     3061 tgttcacttt gttttttctg aaaaaatcgg tgataattcc taccgcctgc tgcttgctgt
     3121 acacgtcgtt tttgctgctt actaccagtt ctacgttgtc attcagataa gaggtgagtt
     3181 tcgctgcatc gccggtgttc agcgcggtga cgatttcagc cgggaaaccg gttgtttgtg
     3241 ccggaacagc ctgagtgagc agagtgaaaa aggctatgaa tgttaatatc tgtattgttt
     3301 tcattgattt ttttggtttt tacctttcaa aacagctgtt tttatctaaa aattttgcaa
     3361 tatttgcatg atgataataa ccaaccatgc aaaggtatta ataattgatt aataaaattc
     3421 tgagttctgt ggttattttc tcattaaatc atacaaaaag cagaccaaaa aaacttaaaa
     3481 ggttaattct gtgaagataa aattgataat ggttggtaaa acaacagaca aaatattggc
     3541 aggtttgatt gatgaatacg ctaacagact gaagcattac attatgtttg agcagataat
     3601 aattcctgaa ctcaaaaatg ccagaagtct gacttttgag cagcagaaag agaaagaagg
     3661 gctggctatt ctggcaaaac tggataattc ggatgaggtt attttactgg atgaaagggg
     3721 aaaacagttc agttcggtcg attttgcagc attaatttcg aataaaatgg ttttttcgaa
     3781 taaaagtatg gtttttgtag taggtggccc gtacggtttt tcaccggatg tgtatcagcg
     3841 ggcagattcg aagttatcac tttctaaaat gactttttcg catcaaatga ttcgtctgct
     3901 tttcgccgag cagctatacc gggcaatgac cataataaaa ggtgaaccat accaccatga
     3961 gtagaacaaa gaattttttt gacagaatgc atttgtcgca caagatattg cttcttgtga
     4021 tgctgttgct tgtaaccggt ggatttgtct ttcagcagat ttacaaaaac tcagcgaagc
     4081 acgctttctc cattcgtaat tttaccgggg aacttcatca gcgggaatac aaggcagatc
     4141 tgaaaattaa ggagattgaa ggtttgctcc ggaaagagaa cacagaccgg ctgccctata
     4201 tttcgttcga aaaacttcct tccgtttacc ttgtgtacaa aaacgaccag cttattttct
     4261 ggtctgataa tcagactgaa ccacaaaatc tgaaaaatga aaaatggcat tatgcgcttc
     4321 tgtccaacgt gcatgctatt gcgcgttcgc atcagaccgg ggcatacaat atcgtagcat
     4381 atatacctgt aaagtataat tatcaatatg agaacagggt gctgaaaaat gttttttcgg
     4441 atggattcag aatgaataag aacatcggga tagtatccaa caatccggca agctccggcg
     4501 cagttttcag ccggcagtcc gattaccttt ttacccttga ggcacccgaa aaaccccttt
     4561 ataacgaagc ctgggctatt acctccatga taatgtttct gattgctttt ctgatacttt
     4621 tttatctttt tgcccgtctt ccgttgcttt acggattatc ttatatttca tggaaaaact
     4681 ttcttgttgc aggtttgctg atggctgttt ttgtttttac atgtttgaat ttcaattttc
     4741 cggcttcttt ctttttgaat aaagttttca ctccgtttca ttacgcatca aatatatttc
     4801 taaaaacact tactcaccta agttttcttt cgttgtttct cttttcactg gtttatttgt
     4861 atacttacca cgtgaaagca gagttgacgg aggataggaa taacaaaatt aagcaaatag
     4921 cattgctgct tattccgggt tcttttttca tgctgatatt tgtatttctg aaaagtgtcg
     4981 ttttcaattc atctgaagat gtaaatatcc tgaagctgga agatatcaca ctggtagctg
     5041 tatggaatca cctgctgttt ctgctgtggg gcatcagcta catgctgctt cacgccaggt
     5101 ctttccgcat actgctccga aaaaataaca tcatcaccct tgccaggctg gattttttcg
     5161 tgtcagtttt cgtctgtctg gcgggttacc tgatgtttga caaatacggg ttgttgtttg
     5221 tgactgctta catcgtgctt actgccgtat tgtatcttcc ttatgttttt acaaaattga
     5281 tgggtccaaa tctgacaacc gttgtttggc tgatgttttt cacgcttttc attacctggt
     5341 cttctgtgac aatgaatcag gataagaaat atgcaaaata ccggacgctg gccgaaaacc
     5401 attacctgaa cgaaggtacc gatgaagacc gctttgcgat cgctttgctg gatgacctga
     5461 attacagcat caggaatgac aaaaggatta agcacctggt gcaattcccg gactcaatca
     5521 gtgttgcaaa tgaatatctg aataaaaaat atctgcgggg cttctggaat aagtatgata
     5581 tgcgactctt tgccacatta ccggattcgg aacttgatat gcagtacaac cagcaaatct
     5641 caaattgggg cagacgtgtg aagaatacaa atttctactc aatgggtaat ccggattcag
     5701 acatgtcgtt ccttggtgtt ttttctgttg tgaaaaatga cagggcaagc actgatttct
     5761 acatggaatt ttatcccaaa aaatacttca aaagttacag cttccccgat ctgctgctgg
     5821 atactccacc gggtattcag tcaaagttca atctttccac ggcacgatat acgttcagag
     5881 aactggtgaa ctctgccggt agcttccagt atcccggtga tgccggatgg gtggaaaagc
     5941 gaaaagagaa ttatttttcg caggtttacg cgggttacag gcactacatt tatgtgccca
     6001 atgtgtacaa ttattttatt ctatctgaag aaaatgcaac aggaacaaaa ttcatcattt
     6061 attttttata tacatttttg ctttatctgt ttatctcatt ctttaccctg tggatttaca
     6121 gattgattca tcgtcaaacc agaattcgtt tcaatttttc ttcaaaattt ctttattcat
     6181 tcaccatttt gctggggttg agtttcctgt caatattcta cgtatcagtg aattatatgc
     6241 aacaaaagta tgtggatgat caaaaacaaa aactggagga aaccaagaaa tacatccaga
     6301 gtgcgctcca ggaaaaatat tcctggcgcg aatcactcga ttcaaccctt accagcgatc
     6361 ttaatttcga tttgcaggac ttgtcatata cttaccagac cgatattcat gtgtatgaca
     6421 ataagggaag gcttattgcc agttcgcaga tgccgctttt ctcccgtgag ctcatcagtc
     6481 gccagatttc accactgcct tttttctcca atgtactgaa tctcaatcag tatgaacata
     6541 tcggtaatct ggagtatctg attgcctata ccgattttta taatattgat tatttaccaa
     6601 taggttacat cgcagttccg cagtttctga ccaacgatcg tgtgaaagaa gatctggaga
     6661 gttttctatc cgttattatt aatatctatt tgattattat tgttttattt attgcattga
     6721 gtctgtttat cggccgtcag ctgtcatcgc cgcttttaat gcttcaggac agcctgaaga
     6781 agattaaact cggacaaaaa atgcaaaaaa tagattataa acccaaagat gaaattggtc
     6841 agctcgtgga gcaatacaac cgtacggttg aggaattgga gaaaagtgcg caattgcttg
     6901 cccgctccga acgggaatcg gcatggaaaa ccatggcccg tcaggtggct catgaaataa
     6961 ataatccgct tacacccatg aagttaacca ttcagcagtt gcagcgccgt aaagcaatga
     7021 acgatgaaca tttcgatgaa tattttgaaa aatcgtcatc aacactgatt gaacagattg
     7081 agaatctttc aaaaatagca ggcacattct ctacttttgc ccgtttaccc gatgcaaaat
     7141 tcgacaaagt ggatgtggcc aggaagttaa gttcggtagt gattcttttt tcaaacaaca
     7201 acgaaaatat gaatattacc tacgaaggac ctgacgaaaa tatctttgta tgggccgatc
     7261 aggaacagat gatacaggtg tttaacaacc tgctgaaaaa cgctattcag tccatacctg
     7321 ctacccggcg cggagaaatc ggggttaatc tggagttaac cgaaaagtgg atacatattt
     7381 atataaagga caatggaaaa ggtatagccg acgaaataaa agacaaactt tttactccga
     7441 atttcacaac aaaaaccacc ggaatggggc ttgggctcac catcactcag agtattgtga
     7501 tgatgtttgg cggcggaatc acatttgaaa ccaaagagga tgaaggtaca actttcaaaa
     7561 tagctctgcc gcgaatttat taaccggata ttagtgtttg gtcattttta ccactgtcca
     7621 gttgtttttc cggttgaaat aatccgtctt caacccgtat gctgccgctt tcgactcgat
     7681 aaccggtaaa tcttccgtat aaaatccgct catgaaaagt tcgccaccgg caggcaggca
     7741 tgcggagtat ttttccatgt cattcagcaa aatattccgg ttgatgttag caataattac
     7801 atcaaaatgc tgcccggtga gcagcgatgc gtcgccgagt aatacccgga tgttgtttac
     7861 gtcattcaga gctatattct caagtgagtt tcttacacac cattcatcga tatcaatggc
     7921 cgtaatggac tctgcaccct tcagcgaagc cagtatggca agcaccgaag tcccgcagcc
     7981 catatccagt accgattttc cggtaagatc cattttcaga atttcagata ccatcagcga
     8041 agtggtttcg tgatgccccg tgccgaatgc catcttgggg tcgataatga tgttatattt
     8101 cagtttcggt atgtctttgt gaaaagaact gtgtatgata cattcgttgt caatggttat
     8161 gggctcgaaa aaatgctttt cccattcttc gttccagttt ttgtcttcca tctcttccgc
     8221 aacgtaactc atagccacat ccagtggaaa acgcttcaaa gcagtctcaa attcctgctc
     8281 actgaagttt ttctcaggaa tgtaggcgtc gagtccttca tcggttgtta cgaaactttc
     8341 aaaaccgatt tttcctaaat aatctgctaa aacatcggca acaaaatctt ctttgttgtc
     8401 gaatcggagg tgaatggcca ggtatctcat attttctgaa attttacaga acaaaattac
     8461 aaaataaatg ttggaaaaag agtaatttta catccgaaat aacgtctccg gatcttattt
     8521 gttaaagttt tcaacagact ttcaaatggc atagttgttg atgcatatat gatgtttaat
     8581 gtttttaatc accaattaat cttttacgca aaatgaaacg ctttttaatt ttaattaatt
     8641 taatcatttt tggtttgtcg ctgggatttg ctcagtcagg caaaatcatt gacgacatat
     8701 acgttacgcc aaatgatgct cagatggtga atgaagtgaa atcggccgta aaatccaaag
     8761 cgaaaaaacc cgaaacaccg caatccacta tttatagaaa cggtgccaga gaaatcgtgt
     8821 tcattgatca atacggaaac aggactaccg ccgtttcgga taccgtgtat atcgtagatg
     8881 gcgaaatgat tgccgatacg cttgcagccg acagtattgc cgacgaagaa ggatattatc
     8941 tgaatggttt ttcgggctct aaatccgatt tggagtacgc cgaacgaatt cgccgtttcc
     9001 acaatccgaa atataccatc accatagccg atccgggtta taatgacatt tacttcctgg
     9061 atgaaaatta ctggaacgta tatatcgatg gcatgtacgc cacaattaca cctacgtgga
     9121 ctaatcccta ctggttcaac tatcagtact caccctacag ctacaacagc tgggcatgga
     9181 gacacaactg gggttacccg tattccggct ggggtggtgg atggtatgat ccgtggtatt
     9241 ccggttacta cggctgggga ggatattatg atccgtggta ttccggcatg tatggctggg
     9301 gctatccata cggtggctat tatggctggg gtggctacgg aggctggggc tatccgtatt
     9361 atggcggata ctatggctgg ggtggctatg gcggttgggg atacccatat tacggtggcg
     9421 gctggtatga cactaccaat cagacaagca ggagaaatta tgccggcgga cgatcggctt
     9481 ttgactcaaa tacatcatcc agacgcagct cagtaatcaa ttcggctgca ggaaccagca
     9541 gaagcggata tacatcggta agtcgtcagc cttccgctac aacaagtcag cgtactgctg
     9601 caacaacttc acgtacaacc agatcgtcgt actcacccgg tgtagatacc cgcatgagaa
     9661 caggcagtgt aagatccact caggaatgga gcaatgtgcg cagcaacaat acccgggttt
     9721 attcatcgcc tgaggccggc gaaagatcat catcggtgat taaccgcacc aatccggcta
     9781 ccagaagcag ttcggtgaat tcaagctcag gcagaactta cgaatcgaga agttcgtcat
     9841 ctgctccgtc gcgcacgtac gacagcaatt cgggcagctc cagaagcagc actcccacct
     9901 atactccttc ccgttcatcc gattcgggca gttcgagaag cagcggttca tactcatccg
     9961 gcagcagcgg atcttactca tccggtagca gcggtggtgg cgcaagccgt tcgtcaggtg
    10021 gcggcggaag aaggtagtac cgattttttt atcagtttga aattcatttt attaaaaaaa
    10081 taaatatttc ttattcaact tacatctaac ttataaaaaa aatgaaaaaa tatatctttg
    10141 ctgctatctc cctgagcctg agcctcctgg caggtgcaca aacagaatac gatgctttac
    10201 ggctttcaca gtcagaactc accggaactg cccgctacat gagtctgggt ggtgctatgg
    10261 gggcactcgg cggcgatgct tctgccatga aggacaatcc tgccgctctt ggagtgtaca
    10321 ggagtaccga gattaccggt acaatgaatc tgggcctgaa caataatcag ccaataacct
    10381 ggcagggcag gactacaaac caggaaagtc gttcagatat tactttcaac aatttatcct
    10441 acgtgatgac catgccggta agcgaaggaa agagtctggt tggttcaaac ttttcgtttt
    10501 catttcagaa actgaatgat ttcaataaat ctttcacaat caacggaaat aaatcaccaa
    10561 gatcatttac cgatttcctg gcgggttttt cgagccccaa tccacctata accggtgatg
    10621 tgtcgtatga taactacgat atgccctggc ttacagtgtt gggatttgac ggatatctga
    10681 tcgatatcga tggtcagtat ttcaagtcgg tgctcaacca gggcgaactt gtcactccat
    10741 cgtatctttt cgatcagtat ggtgctctca atgagtattc ttttggctgg ggaggcaatt
    10801 tcaacgataa tcttttcatt ggagccaacc tgaatatccg gagtctggac tatacacttt
    10861 cgagcacact gagtgaaagt tttgctgaag gaggtggttt tgatctggtg aataaactga
    10921 ctcaaagcgg aatgggactg aatgcaaaat taggcgctat ttaccttcca aacaatagtc
    10981 tcagacttgg aatagccttg catacacctt ccatttcata ttttacagaa gaaagttatg
    11041 ccgatttgaa ttccggctta ataccgaatg atgaaaaaaa tcctgcccaa acgccggtta
    11101 attcccaatc gttcaatctt tggagtccga tgcagctaca ggcaagtgca gcattattgt
    11161 tcgggaaaac gggctttatt tcggctgaat acaattttgt gaattaccgc ggggccagat
    11221 tcaacggtaa ttccaacagc gtgcaaaatt tcggagatat taacatggct atgaaagatg
    11281 tactcaataa tgtgcatgta ctgaaagccg gagcagaatt aaaagtttcg ccgagtttct
    11341 cgctgcgcgc aggttatgcc atgatgactc ctgccgtgaa taccggctac aatgacggta
    11401 agctgctcgt gagaaacgct gtgaatacaa atacagagta ttttgatcaa aaatataata
    11461 caacttatat gacttttggt ttgggttaca ggcaggcaag ttggtttatc gatctggcct
    11521 acgcattgag aaatcaaaaa gaagattttt atccttatca ggacaaagca ctcccgccgg
    11581 cagtgattga aaataaaaca aataacattg ccttaacgct gggattgcga atgtaatacc
    11641 tccaactgca tattcttaga aaaagccggg ctgtacgacc cggttttttt gtgtaattac
    11701 ccgaaaaaca gtacctttgc acctcatttt ttttgagcag aaaaaacagc tgatttattt
    11761 tggtaaaaac ctgtaaatca aatacagaaa atcagcacaa taaacctaaa acgctcaaca
    11821 tccaacatac aaacaatgag tttactatca gaaataaatc gccgacgcac atttgccatt
    11881 atcagtcacc cggatgccgg taaaactacc ttaaccgaaa aattactctt gttcgggggc
    11941 gccattcata tagccggagc cgttaaatcc aacaaaatcc ggaaaacggc aacatccgac
    12001 tggatggaaa tcgaaaaaca gcgtggtatc tctgtggcaa cttcagtaat gggattcgaa
    12061 tacgatgatt acaaaataaa catccttgat acacccggac accaggattt tgctgaagat
    12121 acctaccgca cactcacagc tgtagatagc gtcatcatcg tagtggatgc cgccaaaggt
    12181 gtggaaaccc agacacgcaa actgatgaat gtttgccgga tgcgcaatac acccattatt
    12241 gtttttgtga acaagatgga ccgcgaaggt cgtgatccgt ttgatctgat ggatgaactg
    12301 gaagcggagc tcggtatcaa tgttcgcccg ctgagctggc caataagcat gggagctaca
    12361 ttcaaagggg tatataattt tttcgaacag aatatgcagc tttacactcc gaataaacaa
    12421 ttcataagcg aatcggtgga aattgatgta aacagctccg atttggaaaa aaaagtggga
    12481 gaaaaggatg cagccaaact tcgcgaagat atggaactta ttaatggagt ttatgctgat
    12541 ttcgataagg aaatgtatct caaaggtaac ctggcgccaa tatttttcgg tagtgcactg
    12601 aataattttg gtgtaaaaga attgctcgat tgttttgttc ggattgcacc ttcgcccaga
    12661 cctgtacaga ccacggagcg tatcgtagaa ccaaatgaag aagctttcag cggctttatt
    12721 ttcaaaatac atgcaaatat ggaccccaac caccgtagct gcatcgcatt tgtgaagata
    12781 tgttcgggta aatttctgcg caacgtgaat tacaagcata tacgcaacgg aaaaatgatg
    12841 cggttctcca gcccgacggc attcatggcg cagaaaaagg aaaccgtgga tgaagcatac
    12901 gccggagata ttgtcggatt gcccgataca ggaaacttta aaataggaga caccctgact
    12961 tcgggtgagg acttgcattt caaaggtatt ccgagttttt cgcccgaaat gttcatgtat
    13021 ctggaaaatg ctgatcccat gaaatccaaa cagctggaaa aaggcatcaa tcagctgatg
    13081 gacgaaggtg ttgctcaatt gtttgtaaat caatacaata atcgtaagat agtgggaaca
    13141 gtcggtcagt tgcagttcga ggtaattcaa taccgcctgc tgcatgagta tggagctcaa
    13201 tgccgctggg aacctgtttc gctgtataaa gcctgctgga ttgaaagcaa caatgctgtg
    13261 gaactggaaa atttcaaaaa acggaaaatg caatacatgg caaaagacaa agagggaagg
    13321 gatgtcttcc tggcagattc caattatgta ttacaaatgg ctcagaatga cttcaaagat
    13381 attaaatttc actttacatc tgaattttaa aaataagtag aatgagaaaa tcaatcattt
    13441 tagcaatcct tttatcggta actttatcct tttttgctca ggaaaaacgc gacaaaccca
    13501 ttccggttga tctcttattc ggcgataaac gggtaaatct catgttttcg atgaataaga
    13561 ctatcgatgg aaatttccgg tttatcaatt ctaccagtgc agctgcctat tacgactatt
    13621 tgaagggcaa aacagaaatg gtttccacca gttctattgt gtatcagttt cataaaaaca
    13681 tcggtatttc gggtggtatg caatatcatt ttgccaaagg atttattccg aatatcgcta
    13741 tgcatttttc gcacggaaat cccacctggc tgattgcact caccccatat tacaacttca
    13801 tgccctggaa tagcctcgaa gcagcagcaa tagccgaatt caagccggca cttgctgata
    13861 atttacgtct tttcacccgt ttacagggct tttattccta tgatttgaaa aaagacgaac
    13921 gtgaacgcgg aataattaac tttcggttgg gactcaccat gaagaaatac accgtcggtt
    13981 tcggtggtgt gatcgattat taccgtccca cagcgaacga aattcagaac tacggtggtt
    14041 ttgtaagggt ggaaatttaa tacccccgag tcccggggat gggatattga gagatgttac
    14101 cagacaatga aacataaaga taaaaggcat aaaaaaaaca gatttgataa tctgtttttt
    14161 tttgtttcca ttttgagaga ttaggggtct ttttcacaac ttgctctgca aacttatttc
    14221 ttttttcttt tttccagata gtctttggtg gcctgtttta tttttgaagg aacttcctct
    14281 ttttctttca gaatttgggc agctcttcgt gccgctcttt tttcgttgat tttttgtttc
    14341 agaaattgaa cgtatggttc aatatatcgt tgtcgttttg attctgtcag gtcgtccatt
    14401 gtaagcatga tagcggactc ataggtccct ccaaagtccg gatcaatcat gcttccgaac
    14461 atgcgaagcg tgtcggtgag gccaatgtat gcattaaaca tgggtggaac ggtagtgcca
    14521 atgtgccgta tggttttttg taatgccttg tagttgcctt cggcattttt ttcagtcaaa
    14581 acaacctgtg caattttttt gctttcttcg ctcacagcta ccggaatttt cggatagatc
    14641 agattatcgg gatcaggaca gtgaagaaac atgtattcat acaataattc gcgtgccagc
    14701 tgtggatagg tgttgtaaat gttcactttc ccaacgaaat acttcgattt tttttcagta
    14761 tgtatcaggg cgcccaatcc atcccagagg ttgtcaagtg caaacagact ttttacaccc
    14821 attttagcag tctggtagtc gggttgaaca aatgcgcgac cgagttcgat gcaaaatggc
    14881 ataaaatctt tcataaacct gtcactgaat gtaaagagat gcgacatgac aaaattcggc
    14941 tgaccgtccg gtagcagttc cacatcacga ccagaaagaa accggtatcc accgacaatt
    15001 tcttcggctt gcggatccca gacgatgagt tgtttgaaag gctttttcaa aaaatcaaaa
    15061 tcatccaaat ccagttcatt gcccgtacca ccaccccacg accggaagga gagttcacgc
    15121 agacgcccta tctcgcgcat tacattcggg ctgtcgtgtg ctgtgacaat gtaaatgtcg
    15181 tttttaccct tgttggtagg ccttacatac cgtatttcag taagctcttt tttcagcaaa
    15241 cttctgtcaa cgggtggaat aatttcttta ttcattatca ggttttttca ttgtagattt
    15301 atctcacaaa agtaatattt tttattttca atcgtacatt ttgctccgtt tttaaatttt
    15361 gacgtgtaag tgtaatattt ctcaaaatta aaatacagca ggttgatccg gaaaattcat
    15421 cctgtttgat taaatggcac gttaaaaaca aattatccga attctgccaa gtttaacaaa
    15481 ccttttgtta tatttgtaac ataaaataaa tgatacataa caaaaaatga ttcaaataaa
    15541 aaaaacaggt agctatgaaa aaaatattga ttacagccgt tgtaatattt tcttgtatgg
    15601 cagtaatgaa cgcacaaaaa aatgaacttt ttctgaaaaa agaatttgtt tttaagggcg
    15661 acacgctgaa atacagggtg ttattccccg aaaattacga ccggacccgc caatatccct
    15721 tagtggtttt ccttcatgga tccggtgaac gaggtaacga caacgaaaag cagctcgtac
    15781 acggtgcttc gttgtttacc gattccgaaa accgtaagga ttatccctcc attgttattt
    15841 tcccgcagtg tcctcaggaa gacttctggg tagaaagaga gaaaattgat tttgaaaata
    15901 tcgaatttcc caaaaaacct aaaattacaa aatcacttta tctggtgaaa aaactgctcg
    15961 atagttacaa gaaaaccgaa gctgtggacg atcataaaat atatgtgatg ggactttcca
    16021 tgggcggcat gggcactttc gacctgattt gccgctatcc gaaatacttc gcagcagcca
    16081 tacccatttg tggcggagtg aatacagaac gtctcaaaaa agtaaaaaaa atgcccatcc
    16141 ggatttttca cggaggagcc gatccggttg tttcacggga attttcgcgc aatgctcaca
    16201 tagagcttaa agtacagggt tcggtaaagg tggagtatat cgaatttccg ggcattggac
    16261 acgacagttg gacaaaagca ttccaatacc ccgatttcct gagctggttg tactatcagt
    16321 cacgctaaaa ataagtaaaa agtaactaaa ctgcttacat tctgtcattt tttggaatgt
    16381 gaaataagtg aaaaaagtga aaaaataaat catttagcag tcaatatctg tttttttggt
    16441 tacttttgtg aaacaaactc aatttttaag ttgaaataaa catggcaaaa ctaaaaggtg
    16501 ctgtaatcgt taacgtagag cggtgtaaag gctgtaatct gtgtgttgtg gcatgtccgc
    16561 aggatgtatt attgctcaac aaagaagcaa actccaaggg ttacaattat tcatatatgg
    16621 aaaatccgga agaatgtacc ggttgtacta cctgtgctct ggtgtgcccg gatgcctgta
    16681 tcaccgttta ccggatcaaa gtggaagaga aaaattagat ttttaaaaat taagattttc
    16741 aattagatgg aagaagtaac attaatgaaa ggaaacgaag ccgtagctca tgccgccgta
    16801 cgtgcaggct gcgacggata cttcggttat ccaatcacac ctcagtcgga aataatggag
    16861 actctcatgg aaatgaaacc atgggaaaca accggaatgg tagtattgca ggccgaaagt
    16921 gaaacagcat caatcaacat ggtgtatgga gctgcaggct gtggtaaacg taccatgaca
    16981 tcttcttcaa gtccgggcgt gagtctgatg ctcgaaggat tttcatacat cgccggagcc
    17041 gaattgccct gtgtggtggt agatgtgatg cgaggcggac ccggacttgg caccatacag
    17101 ccaagtcagg ctgactacta tcagatagtt aaaggtggag gacacggcga ctacaaagtg
    17161 attacacttg ctccggctac tgtacaggaa atggcagact ttaccgtgct ggctttcgac
    17221 cttgcattta aataccgggt aatgtgtgtg gtacttgccg acggcgtagt aggacagatg
    17281 atggaaaaag tggtgttgcc gccgttccgc ccaagatgga ccgaagaaga aattcgccgg
    17341 atgcacccat gggctacgct cggtagaact cccgaccgca agccaaacgt aattacctca
    17401 ctcgctctgc aggccgaaga gatggaagaa attaacctcc gacttcaaag ccgctacaga
    17461 gaaattgaga agaacgaagt gcgttacgaa gaagtaatgt gcgaagatgc agaatatctg
    17521 atcgttggat ttggaataag tgcccgcatc ggtcagaaag cagctgaaat ggctcgtgaa
    17581 gagggtttga aagtaggtct gctgcgtccg attacgctct tcccattccc gactaatgaa
    17641 ataaaaagac tggccgaaaa agtaaaagga gtgcttacag tagaattaag cgccggacaa
    17701 atggtggatg atgtaagatt ggcagtaaac ggtaaagtac ctgtagaatt tttcggaaaa
    17761 atgggtggta tagtaccatc gcccgacgaa gtactccacg cactcaaaga gaaagtgata
    17821 aaatagttgt cccctgaccc cctgaagggg aaataggaaa aatcaacact atgattattt
    17881 tgaaaatcaa tattttataa aataaagttc aaaaagttcc cctcaaacta ttttcctttt
    17941 tctttctcca tttggagaaa gtacccgaag ggggatagag gtaggggatt taggggctta
    18001 acatattatt taaaaaaatg acaacacaaa ttatagaacc tcagaacctg gtatacaaaa
    18061 aacccagttt gctcaatgac agcaacatgc actattgccc gggctgcagc cacggagtgg
    18121 ttcataaaat catcgcagaa ttgattgaag agatggggat tgccgacaaa gccatcgggg
    18181 tagcacccgt tggatgtgcg gtattcgctt acaaatatat tgatatcgac tggcaggaag
    18241 cagctcacgg acgtgcacct gctttggcta ctgcaattaa ccgtctcgtg ccaagtagca
    18301 tggttttcac ctatcagggt gacggcgact tagccgcaat cggaactgcc gaaaccatac
    18361 atgcttgcaa ccgcggagaa aacatcacca tcgtatttat caataacggt atctacggta
    18421 tgaccggcgg tcagatggct ccaacaacgt tggtggggat gaaaactgca acctcgccgt
    18481 ttggtagaga tatcgacctt aacggacacc cgctgaatat caccgaacta cttgcaaaac
    18541 ttccgggaac ttgctatgta acccgtcagg ctgttcacaa agtgggtgat gttcgtaaaa
    18601 ctaaaaaagc actccagaaa gcattccaaa acacactgga taaaaaagga acatctgttg
    18661 tagaagttgt atctacctgt aactccggtt ggaaaatgac tcctgaagcc tccaacgatt
    18721 ggatggtaga aaatatgttt ccggcttatc cgctgggcga tttgaaaaat ttatagtaag
    18781 tagttaagag ttaatagttt acagcatgaa agaagaaata ttaatatccg gttttggagg
    18841 gcaaggtgtg ttgtccatgg gaaaaatttt ggtttattcc ggccttatgc aaggtcagga
    18901 ggtgagctgg ctgccgtcat atggccccga gcaacgaggt ggcacagcca atgttaccgt
    18961 cattctgagc gatgaaaaaa taagttcgcc gattttgaat aaatacgata cagttattgt
    19021 gttgaatcaa ccttcgctgg ataaattcga aagtcgcgta aaaccgggcg gcacgcttat
    19081 ttttgacagt aacggcttca aaagatttcc tgcccgcacc gatatcaaca tctacaaagt
    19141 ggatgcaacg gagtatgctt ataaacatga catggtgaaa acgctgaata tgatgattct
    19201 gggcggtttt ctgaaaatcc gtcccattgt aaaagtggag aatgtactca aaggactgaa
    19261 aaaatcgctg cccgaacgtc atcatcattt gatcccgatg aatgagaaat caattctgaa
    19321 aggaatggat attgttgaaa aat
//
LOCUS       DAORKP010000023        19290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_140_length_19290_cov_9.748843, whole genome shotgun sequence.
ACCESSION   DAORKP010000023 DAORKP010000000
VERSION     DAORKP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19290
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_19290_cov_9.748843"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..852
                     /locus_tag="PLN75_02500"
     CDS             469..852
                     /locus_tag="PLN75_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG03019.1"
                     /translation="MIVLNKIQRPNPLDKDAPKKFYVSASSTGSVDLEEMSVNISERC
                     TITEPDVLAVLSALTSEMTKQLMDGKIVRFGSFGSFQLGVLSHGAATEEETSRNLVKG
                     VRVKFRPGRRISDALGALRFTLKTV"
     gene            complement(1696..3135)
                     /locus_tag="PLN75_02505"
     CDS             complement(1696..3135)
                     /locus_tag="PLN75_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRG03020.1"
                     /translation="MTILRKAITCVLFCSFWFANAQNTTTDYLKHHVFTLAADSLKGR
                     KAGSEESTRAARYIVEQWQAIGIQPYKDNDYFHSFDKYKNIIGIIEGGDENRKNEYIV
                     IGAHYDHLGFKIKDNNTIIYNGADDNASGTAALIETARLLKSRQNELKRSVILVAFDA
                     EEVGLVGSTRFVNDNLFSSASIMLMMSVDMVGWYGTNGAVEYSGTGSFRNSERIMNEA
                     NDIPTGLKVKTEAFEKNMFVATDTQPFARAGIPTLAVTTGLKSPYHKPKDDADLIDYV
                     GMTLITEHLANLTIKLSQEDEITPTGKVADKHLSSRKLFQMGFTGNIGNNHHYYTDGA
                     LDGKSSFAYGIGLTTQLNLGALAFRPEVFFEDVSAKYPGGNIHSNNITIPLNLVLQSK
                     KPTGGVDVFVGSYYTYRLNGKQGTEPLDFNTFNRSEWGINWGFSFLAGPVKIGYTNRS
                     ALTNFTQQPNDDNAYVRNRAGYFTLGWMF"
     gene            complement(3407..5440)
                     /locus_tag="PLN75_02510"
     CDS             complement(3407..5440)
                     /locus_tag="PLN75_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRG03021.1"
                     /translation="MTSKDSLKEKENLNNQILPEEMLQENKTEIETVVPETEQNTVTE
                     PETQTVAETVAEPKPEIAAEPLVETEPEAEAETETEPEVSESEIPVLVPIPVLKSEDE
                     SEEIKASDVTEMSKEQLIERLKELIEQEVEQVKEEVEEIKQLFYRKSKAETDLQRASF
                     IENGGIETDFIPEATELENEFKELLAEYKNKKAKITAELEKERENNLLKKNHIIEQMK
                     LLIDSKEDVSANINTFRQLQQDWKNTGKVPPSANNDLWKQYNQYQESFWDLIKISNEL
                     REYDFKKNLEAKNKLIEAAEILANEEDVVTAFRQLQKLHEDWHELGPVARDLREQTWD
                     RFKAASTVINKKHQAHFDDIRGLEEQNLAAKISLCERIETIDVSSLKTYKEWDEVTRQ
                     ILDLQEEWRTIGFAPRRANQKIFERYRKACDSFFTAKADFYKGVKSNLNENLDKKKAL
                     CEKAESLKESTDWKKTSDEFVKMQKEWKTIGPVQKKYSDEIWKRFISACDYFFEQRNK
                     NVTGQKSEEVLNLEKKKELIEKIKSFKKTDNVSESLAALRALAAEWNNTGHVPFKEKD
                     KIYKEYRTVLDAQFDSLNIDASQRRLESFRSNLKDMSDKGENKLYRERDKLVRAYEHL
                     KSEISTYENNIGFLSISSKKGGGMLQEMERKIETLKEECKLLEQKINLIDEKI"
     gene            complement(5548..6318)
                     /locus_tag="PLN75_02515"
     CDS             complement(5548..6318)
                     /locus_tag="PLN75_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRG03022.1"
                     /translation="MNTALVTGATSGFGRAIALRLAALGYNLVVTGRRKELLDTLLHE
                     INQKYSVEVFALNFDVRDKNACENALNSLPEALRKIDLLVNNAGLAAGAAPFQESDPE
                     DFERMIDTNVKGLLYITKLIVPGMIERNKGHIVNISSIAGIEVYPTGNVYCASKHAVN
                     AITKGLRIDLVKHGIRVSSISPGMAETEFSVVRYHGDREKAKAVYKGLQPLTAEDIAD
                     ALEFIVTRPPHVMINDIQINPSQQANTYISHRIDTVNQ"
     gene            complement(6363..7577)
                     /locus_tag="PLN75_02520"
     CDS             complement(6363..7577)
                     /locus_tag="PLN75_02520"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRG03023.1"
                     /translation="MKEKLDKIEDAIEDIRMGKFVIVVDDEDRENEGDFICAAEKITP
                     EMINFMLKNGRGVLCAPLTEERCDALELEMQAEVNTSIHSTPFTVSVDKLEGCTTGVS
                     AADRAATFRALADPASTPETFGRPGHIFPLRAKSKGVLRRAGHTEATVDLARLAGLNP
                     AGALIEIMNEDGTMARLPQLYEIADEFGLKIISIADLIAYRIRTESLIQRGVEVDMPT
                     AYGHFKLIPFRQLSNGLEHIALIKGTWKHDEAVLARVHSSCMTGDIFGSMRCECGEQL
                     HKALELIEKEGKGVLVYMNQEGRGIGLMNKIKAYKLQEEGLDTVDANLHLGFNADERD
                     YGIGAQILREVGVTQLRLMTNNPKKRIGLESYGLSVVENVPLEVEPNKYNAFYMQTKK
                     ERMGHKLKYVGK"
     gene            complement(7634..9142)
                     /locus_tag="PLN75_02525"
     CDS             complement(7634..9142)
                     /locus_tag="PLN75_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRG03024.1"
                     /translation="MLGIKKIDTYIVKSFLAYFFMTFFISMLILLMQFTWKHMAELIG
                     KGVSWGVLAEFFWYATLQTVPMALPLAILLAALMNFGNLGEHFELTAMKSAGISLFRM
                     MRGLIVFISIISVLAFFFSNNVLPMAQQKLWTLIFSLRQKSPEFDIPEGEFYTGINGY
                     NLYVRSKDPNKKLLRDLMIYDFSKGFDNASVTLADSGRVQFTADNKYLKFSMYSGESF
                     ENMRQQGSRYNSSSIPYRRETFQTKELLLDFDTEFNRYDESILKDQHVSKNVVELTQT
                     IDSVSKIVKARGDEQAREMISTQFPEIRETVSIDEIPMMTDEVSEEQVVTEEEPVKNY
                     AADSLFLKLNQQQMEEAVSAARQSAQAMKDKIDYNRIMLVDPTMTVRRHQIEWHRRFT
                     LSFACLIFFFIGAPLGAIIRKGGLGFPIVISVVLFVIYYIIDTTGYKMAREGFWQVFE
                     GMWLSSAVLFPLGLFLTWKAVTDASFFRTESYTRIYDNMKEYYRKRRKKSRN"
     gene            complement(9213..10598)
                     /locus_tag="PLN75_02530"
     CDS             complement(9213..10598)
                     /locus_tag="PLN75_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRG03025.1"
                     /translation="MLKYEMSELKQTEDSKLSTLSVGKLLWMYSLPAIVGAMVSSLYN
                     IIDRMYIGLGVGPMAISGLALTFPFMNFLMAFSMLVGAGASSRISIRLGEGDRNRAEQ
                     ILGNTLTLTILVSGTVILFSMLFMNPILIMFGGSENTLKYAREFMQIIVPGTILSALL
                     FGFNSIMRASGYPKKAMITMIISAFVNITLAPLFIFVFKMGIAGAAHATNIAFFVGSI
                     WVVLHFFDKKTNIRFHRKNLKLDRQIVKSILNIGMSPFSMQIAASVTVVIINRALIRY
                     GGDLAVGALGIQNAIATLVVMFIIGLNQGAQPILGFNYGARKFDRMFLALKKTAVAAT
                     LVSTAGFILGTFFPHALASLFTRDEELKELAARALRISVTVFPLIGSQIVITNFFQSI
                     GKAKISMTLSLTRQVLFLIPCLIFLPPIFGLDGVWSSMPVADSLSVIVTTSTLVFFIR
                     KFKKRQNSAFI"
     gene            complement(10725..11408)
                     /locus_tag="PLN75_02535"
     CDS             complement(10725..11408)
                     /locus_tag="PLN75_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRG03026.1"
                     /translation="MKFRIVVFLFFSVMMMNLPAQTEKKDTVKSENSGPVFRAEAGYG
                     QNFRYGDYIVTSPYHVLKGGVNVEFPVKWGFGVETGLRYSFAFGNREQIYAHSDTAFF
                     NYSGHYIDVPLRITYTLPIFWGMKLFAFAGPDFNIGLAQTEDVKFTATVLDPAPSNPL
                     NYPVSGTYNLYDNDLRRLNIQLGAGGGLQWKNYRVKSGYDWGINNISQNKNRSQKMKG
                     WYITFEYEF"
     gene            complement(11596..12279)
                     /locus_tag="PLN75_02540"
     CDS             complement(11596..12279)
                     /locus_tag="PLN75_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRG03027.1"
                     /translation="MRLIKFLVPVVLTALLGFNAYAQDKKVEFGIMAGYGYTMPKLKD
                     SRSIKNPAINSSNLNGFHAGPVLKFNFSEQVGLQTGLLFNYFSGVDLLSSSALKKTGV
                     WNQNRIKLTAFDLPLKIMYSMPLADEFYLQIFAGPNFNYSINKVTDYEAYSDNKLNVN
                     LSKKGTNIYQAPSDFNSLDFQMGAGIGVQFMGLFLRGGYDWGILNRTTLDNAKLRSND
                     LKVSVGYTF"
     gene            complement(12420..13025)
                     /locus_tag="PLN75_02545"
     CDS             complement(12420..13025)
                     /locus_tag="PLN75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRG03028.1"
                     /translation="MKTSRKISFVLLAAMLSIGFANAQLGIQAGYSNSKDANSDAALN
                     GFHAGLVYNLPIQGAVSLQYGLLYNYLTTNNDYWGIANSSTTAHRLDLPVRLAVDFPL
                     GGTVSAFVFGGPNFNYALSQSTKVESGLGSLETNNIYKIEMSNGKKHYSPFDLQLGAG
                     AGLKFNQLSVRFSYDWGMLDRNNSDAIWKNNDMKVGIAYHF"
     gene            complement(13277..14434)
                     /locus_tag="PLN75_02550"
     CDS             complement(13277..14434)
                     /locus_tag="PLN75_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRG03029.1"
                     /translation="MKKLFLTVMIIASAITYTSAQTDAAHWSFALKGGVDYFNINPAG
                     DDFIDNASWGAGAALEYTINPIVGVGLNLDYLNFNRSTIKGKTIDPSFFTSVNLSNLL
                     FPKRNSAKLNFYANFGGGVSLPSYSDYVAPTTTYARGMSAMPTKTNALAYTGASMEYN
                     LGRVVALGLDYTYRGYITPLTNVVEYNDANTLMATLRFKLGTGSKTHVRDMTTADFYP
                     APAPVIQHVENAFDPSAINSRLDGLDKAGMDLQNRLKNLEDAVKGLKDKEEGSMISAS
                     FDNIEFDFNSAKLTDASLVILDNVAAILRDNQTWSTLNINGHTDNVGPDAYNQTLSEK
                     RANSVRDYLASKGLSMAAMLTNGFGESKPIADNATVAGRQANRRVEFEITK"
     gene            complement(14792..15189)
                     /gene="ssrA"
                     /locus_tag="PLN75_02555"
     tmRNA           complement(14792..15189)
                     /gene="ssrA"
                     /locus_tag="PLN75_02555"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(15329..16852)
                     /gene="rny"
                     /locus_tag="PLN75_02560"
     CDS             complement(15329..16852)
                     /gene="rny"
                     /locus_tag="PLN75_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRG03030.1"
                     /translation="MIYYVIIAVVALALGFLGAYLMLGVFNKRAKRELEAEAELLKKK
                     KEIEAKERFIALKAEHEQQVQQRNAKVQAAEVKIQQREMQLSQRQSELQRKMNEVDAK
                     SENIEQQLTALENKKRETEHLNRQAKEQLETISGLSAEQAKERLVETLKEEAKTNALS
                     YINEIMDEAKMTANKEAKKIVVQTIQRVATETAIENSVTVFHIDSDEVKGRIIGREGR
                     NIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEIVN
                     KVRKQVEEEIIETGKRTTIDLGIHGLHPELIRMVGKMKYRSSYGQNLLQHSREVANLC
                     ATMASELGLNPKKAKRAGLLHDIGKVPDDEPELPHALLGMRLAEKYKEKPEICNAIGS
                     HHDEVEMETMLAPIVQVCDAISGARPGARREIVEAYIKRLNDLENLALSYPGVIKTYA
                     IQAGRELRVIVGAEKLDDKETELLSFDIAKKIQDEMTYPGQVKITVIRETRAVSYAK"
     gene            complement(17001..17291)
                     /locus_tag="PLN75_02565"
     CDS             complement(17001..17291)
                     /locus_tag="PLN75_02565"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRG03031.1"
                     /translation="MEDNKTFTINILLNGVRMPLNIRREDEEIYRNAEKIVNKYLEQY
                     RKQFSQRSMEEILTLTAYQLAVIVAKQATNQDQAPLAEKIEELNKELIRLMS"
     gene            complement(17296..17643)
                     /locus_tag="PLN75_02570"
     CDS             complement(17296..17643)
                     /locus_tag="PLN75_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03032.1"
                     /translation="MFFLFKAKPYFCGDLVKSMENRYADLIREFESNIRKLISEQKAL
                     REKNATLAAELDGKKRDLMEAHKEILDLQKEYKLLQTANGLGGSDENRRNSRQHLNKI
                     VREIDKCLALLSE"
     gene            complement(17681..>19290)
                     /locus_tag="PLN75_02575"
     CDS             complement(17681..>19290)
                     /locus_tag="PLN75_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRG03033.1"
                     /translation="SDLMAGIIVGIVALPLAIAFGIASGVSPEKGIFTAIIAGFIISL
                     LGGSRVQIGGPTGAFIVIVYGIIEQYGVTGLAIATIIAGLMLVAMGVLKLGTIIKFIP
                     FPVVVGFTSGIALTIFATQIKDLFGLQIEKVPADFLNKWVVYAQNISSVNWWTLLIGL
                     LSIVIISVTPRFSKKIPGSLLAIILMTVLVYFLKNSFGIQGIETIGDRFTINASIPQA
                     NTYNIDFDTVRTLLPVAFTIAMLGAIESLLSATVSDGVIGSKHNSNTELIAQGVANII
                     TPIFGGIPATGAIARTMTNINNGGRTPVAGIIHAIVLGLILFFLGGLTKHIPMACLAG
                     VLMVVAYNMSEWRTFKSLMRNTKSDVTVLLATFLLTVIFDLTIAIEVGLLLAVLLFLR
                     RISEVSTVSVIQDVVDKTDYVEEGIESEKLEIPKGVSIYEIEGPFFFGVANKFEETMK
                     QVGGSSKVRIIRMRKVPFIDSTGAHNLENLIKMSHKDKTRIVLSGVNDNVREVLQKVE
                     IDKLIGSENICNNIIEALEKSKQLLKE"
BASE COUNT         5240 a         4100 c         3796 g         6154 t
ORIGIN      
        1 aaatgctggt tttcaatcaa atatagatat ttttaaattt ttgtcatgta cttagaaatt
       61 tcatacaaaa aaccattaaa atttaattaa aatacacaag ctcgctgact ttttcgatga
      121 ttaaatggtg taaaaaaagc acttttctca catttttttt gtagaaatct tcaaaaatca
      181 ggcgttttgc agtcattttt agtcattttt agtgcctttt ttattcctaa aatctacttt
      241 ccaggcacta atatatttaa ataatgttaa ttatttatct cataacgccc acacagacac
      301 ttaaaaaggg gtttgcctga aagacgtgaa aattgttggg aaaaaaccga aaatattact
      361 tttaacaatt gtatttaatt ggtatctttg ctttagttca tgcttcacaa caacgtgata
      421 gcgagggctg ctaaccgaat ttattcattt tattgttttt ttgaaaaaat gatagtactg
      481 aacaaaattc agcgcccaaa tcctttggat aaggacgcgc ccaagaagtt ctacgtaagt
      541 gcttcgagta cgggaagtgt ggatttggag gaaatgtctg tgaacatttc tgaaagatgc
      601 accattaccg agccggatgt acttgcggtg ctttctgctc tgacttcaga gatgaccaag
      661 cagttgatgg atgggaaaat tgtccgtttc ggatcgtttg gctcatttca gcttggtgtg
      721 cttagtcacg gagcagctac agaagaagaa acttcacgca acctggtaaa gggtgttcgc
      781 gtgaagtttc gtccgggtcg tcgcatttcc gatgctttgg gagcgctgag atttacacta
      841 aaaacagtgt aagcggaaca gttgagccct ccgataaggg ccgggaaaga tgttttgcct
      901 tgaatcgctt aaattgttgg cgcaagttaa gcagggaaag caaaagtaca cgtaaagatt
      961 acagcgcact tgtacatctg tgcatagaaa gcggcaatca aaaaaggttc attaaacgtt
     1021 cattgacccc tccggggcat tggacgtttt ttttttatta taaagttcag aaaaacaacc
     1081 gtatgttttt taaaaatgtc cgtatgtttt attcaaaaag accgtatgtt ttgctcaaga
     1141 agtccttatg ttttttaaaa acatactgag tcagcataaa accggcactt ttgctgaaaa
     1201 agggcaccat ttcttagttt acctttacaa aaataactga tttgaatatt actcaagcaa
     1261 taaatttcaa caacaattat taattattgt taatcgaagc gctttggggc ttatttcgtt
     1321 cgctgctgta aagtgataga acgatgcgat tttatcgctc gtctactagc ctcaaatgaa
     1381 cacaatgtcc aattatccat tacttcccgc atcatccatt atacaactgc aaagcgtcct
     1441 gacacatttt tagttgctac gtcttttaag gcgtgggtat gtagaaaaaa agatttaagg
     1501 gctttagcct aacttatata aaactttggc tgaagccaat ctcttaaatc cattgaatcc
     1561 aagcctctga aaggcgtggc aatggattat caggtcatca tgcggatatt cataacatat
     1621 ttatctgact tactacgcgt taaattagcg tggcagcagt gggtcatttg ggtttctttt
     1681 cttttcaaaa aaagtttaaa acatccaccc aagagtaaaa tatcctgctc tgtttcgtac
     1741 gtacgcgttg tcgtcgtttg gttgctgtgt aaaattcgtg agcgcactgc gattagtata
     1801 accaatcttc acaggtcctg ccaggaagct aaatccccag ttaatacccc actccgaacg
     1861 attgaaggta ttgaaatcaa gcggttctgt gccctgtttt ccattgagac gataggtata
     1921 gtaagaacca acaaatacat caacgcctcc ggtaggtttt ttgctttgta aaacaaggtt
     1981 cagcggaatg gttatgttat tcgagtgaat attgcctccc ggatatttag ccgaaacatc
     2041 ctcaaaaaaa acctcaggac ggaaagccaa cgcacccaga ttcaattgtg tggtaagacc
     2101 gataccatag gcgaacgagg atttaccatc cagcgcgcca tcagtataat agtggtgatt
     2161 gttacctatg ttgcccgtaa agcccatctg aaaaagttta cgacttgaaa ggtgtttatc
     2221 ggctactttc cctgtcgggg taatctcatc ttcttgcgat agttttatag tcaggttggc
     2281 aaggtgctct gtgattaatg tcataccgac gtagtcaatc agatccgcgt cgtctttggg
     2341 tttatggtaa ggtgatttta atccggtagt gacagctaac gtaggtatac cggcccgggc
     2401 aaagggctgg gtatcggtag ctacgaacat gtttttttcg aaagcttcag tttttacctt
     2461 caaaccggtg ggaatatcgt tcgcttcatt cattattctt tcgctgtttc tgaaactgcc
     2521 tgttcccgag tattcaacgg ctccgttggt accgtaccat cctaccatat ccacgctcat
     2581 cattagcatt atactggccg aagaaaacag gttgtcgttg acaaagcgcg ttgaaccaac
     2641 caaaccaact tcttccgcat caaaggcaac aagaataaca cttcgtttta gctcgttctg
     2701 cctgctcttt aacaaacggg cagtttcaat cagcgcagct gtacccgaag cattgtcatc
     2761 cgctccgttg taaataatgg tattgttatc ttttattttg aatccgagat gatcgtaatg
     2821 tgcgcctata acgatgtatt cgttcttacg gttttcatcc ccgccttcaa tgatgccgat
     2881 gatgtttttg tatttatcga atgaatgaaa ataatcgttg tccttgtacg gttgtatgcc
     2941 aatagcctgc cattgttcaa caatgtatcg ggcagcccgg gtactctcct ccgaacctgc
     3001 ttttctgcct tttaacgaat cggcggcaag tgtgaagaca tggtgtttca gataatcggt
     3061 agttgtgttt tgggcatttg caaaccaaaa cgaacagaat aacacgcagg ttatagcttt
     3121 tcttaaaata gtcataataa cgaatggttt atttaaattt ttacttcttc taattttaca
     3181 tcaccctttg ctgtcgcact tttgcatcgt gtggtcaacc agtatattca atctataaac
     3241 ttttactttc tgcttcgcta gcaggcgcag gtctgtgacc tgtgccaatt tcaacctctt
     3301 atttgtattg ccacggattg taaataaatc cgcaccagcg ggggcgagag ccggataaaa
     3361 aaagcgctga aattcagcgc tttttctgtt ttaaacaaat ctaattttaa attttttcgt
     3421 caatcagatt gattttttgc tccagcaatt tacattcttc tttcagtgtt tcaatttttc
     3481 gttccatttc ctgcagcatt ccgccgcctt tcttggatga aatactgagg aaaccgatgt
     3541 tattttcgta agtagaaatc tcggatttca gatgttcgta agcgcgtacc agcttatcgc
     3601 gttcgcggta aagtttattc tcgcccttat cgctcatatc tttcaggttg gagcggaaag
     3661 attcgagcct gcgctgtgat gcatcaatat tcagtgagtc aaactgagca tctaatacag
     3721 tccggtattc cttgtaaatt ttatcctttt ccttgaaagg tacatgtccg gtgttgttcc
     3781 attcggcagc gagtgcacgt aatgctgcga gcgattcgga tacattatcg gtttttttaa
     3841 aggattttat tttttcaatc aactcttttt tcttctcaag attcagcact tcttcgcttt
     3901 tctgacccgt cacattttta tttctttgtt cgaagaaata atcacaggca ctaataaatc
     3961 gtttccagat ttcatcggaa tattttttct gaacgggacc gattgttttc cattctttct
     4021 gcattttcac aaattcatcg gaggttttct tccagtcggt actttctttg agtgattcgg
     4081 ctttttcgca aagtgctttt ttcttgtcca gattttcatt caggtttgat tttacacctt
     4141 tatagaaatc tgcttttgct gtaaagaagc tgtcgcaggc tttccgatag cgttcgaata
     4201 tcttttgatt ggctctgcgc ggagcaaaac caattgtgcg ccattcttcc tgtaaatcaa
     4261 gaatttgacg ggtcacctcg tcccattctt tataggtttt cagagaactc acatcgattg
     4321 tttcaatgcg ttcgcacaat gaaatttttg cagccagatt ttgttcttca agaccccgga
     4381 tatcgtcgaa atgagcctga tgttttttgt ttattacggt tgaggcagct ttgaaacggt
     4441 cccaggtttg ctcacgcaga tcacgtgcta cgggccctaa ttcgtgccag tcttcgtgca
     4501 atttctgcaa ttggcggaat gcagttacca catcttcttc gttggcaagt atttcagctg
     4561 cttcaatcag tttgtttttg gcttcaagat tttttttgaa atcgtattct cggagttcgt
     4621 tgctgatttt tatcaaatcc cagaatgatt cctggtactg attgtattgt ttccagagat
     4681 cattattggc cgatggaggt acttttccgg tatttttcca atcctgctga agctggcgga
     4741 aagtattgat attggcagac acatcttctt tactgtcaat gagaagcttc atctgttcga
     4801 tgatgtggtt ctttttcagt agattgtttt ctctttcctt ttcgagttcg gcggtaattt
     4861 ttgccttttt attcttgtat tctgccagca attccttgaa ttcattttca agttcggtgg
     4921 cttcgggtat gaagtctgtt tcaattccac cgttttcaat gaaggatgca cgttgtaaat
     4981 ctgtctctgc tttgcttttt ctgtaaaata gttgtttgat ttcctcaact tcctctttga
     5041 cttgttccac ttcttgttca atcagctctt taagcctttc aatcaattgt tcttttgaca
     5101 tttcagtcac atcggacgct tttatttctt cagattcgtc ttcagatttt aatacaggta
     5161 ttggtacaag aactggtatt tctgattcag aaacttcagg ttcggtttcc gtttccgctt
     5221 cagcttccgg ttcggtttca accaatggtt cggctgctat ttccggtttc ggttctgcta
     5281 ctgtttctgc aactgtttgc gtttcgggtt ctgtaactgt attttgttct gtttcaggca
     5341 caacagtttc gatttcagtt ttgttttcct gtaacatttc ttcgggtaaa atctgattat
     5401 tcaggttttc cttttctttc agagagtctt ttgaggtcat tttcattcat tttttaagtt
     5461 ccggaatttc tactccggac atcagtacca tataacagag acaaaagtaa gacttttttt
     5521 taaaataaaa tacagatttt tactatttta ttgatttact gtatctatcc tgtgtgaaat
     5581 ataggtgttt gcctgttggg aaggattaat ctgaatgtca ttgatcatca catgaggcgg
     5641 gcgtgtaacg ataaactcca gtgcatctgc aatatcctcg gctgtaagcg gttggagtcc
     5701 tttgtaaacg gcttttgcct tctcccggtc gccatgatag cgcacaacgg aaaactcggt
     5761 ttctgccatt ccgggcgaaa tggaactgac acgtattccg tgttttacca gatcaattct
     5821 caatcctttt gtgatggcat ttacagcgtg tttcgaagcg caatatacgt ttccggtagg
     5881 atacacttct attccggcaa tggaagatat attgactata tgtcctttat tacgttcaat
     5941 catacccgga acaatcagtt tggtaatata cagcagacct ttcacattgg tatcaatcat
     6001 cctttcgaag tcttctggat cactctcctg aaatggagcg gctccggctg caagtccggc
     6061 attgttcacc agcaaatcta tttttcttaa cgcttcgggc aatgaattca gtgcattttc
     6121 gcaggcattt ttatcgcgca catcaaaatt caaagcaaaa acttcaacgc tgtatttctg
     6181 attaatttcg tgtaagagcg tatccaacag ttcctttcgt cgtccggtaa ctactagatt
     6241 atagccgagt gcggcaagtc tgagtgctat ggcacgtccg aatcccgaag tagctcctgt
     6301 aactaatgct gtattcatat atctatattg ttttctgtcc gttagctgtt tactgaaaaa
     6361 aattacttac cgacgtattt caatttatgc cccatccttt ccttctttgt ttgcatgtaa
     6421 aatgcattgt atttgtttgg ttcaacttcc agcggcacat tttccactac cgacaggccg
     6481 taagattcca gtccgatcct ttttttagga ttattggtca tcagacggag ctgagttact
     6541 cccacttctc tgagaatttg tgctccaatc ccataatcgc gctcgtcggc attaaagccc
     6601 aggtgaaggt tggcatctac cgtatcaagt ccttcttcct ggagtttgta ggcttttatt
     6661 ttgttcatca atccaattcc gcgtccttcc tgattcatat aaacaagcac tccttttcct
     6721 tctttttcaa tcaattcaag tgctttgtga agttgctctc cacactcgca tcgcatcgaa
     6781 ccgaaaatat cacccgtcat gcatgatgaa tgaaccctgg ccaaaacggc ttcatcgtgc
     6841 ttccaggttc ccttgattaa tgcaatatgc tccaacccgt ttgatagctg acgaaatggt
     6901 atgagcttga aatgtccgta agcagttggc atatcaactt ctacaccacg ctgaatgagt
     6961 gactcggtac gaattcggta agcaatcaga tcggcgattg aaattatctt caaaccaaac
     7021 tcatctgcta tttcgtacag ttgaggtaag cgtgccatgg ttccgtcttc gttcataatt
     7081 tcaatgagtg ctccggcagg gtttaggcca gccagacgag ccagatctac agtggcttcg
     7141 gtatgaccgg cacggcgcaa aacacctttg gattttgctc tcaacgggaa aatatgtccc
     7201 ggtcgtccga atgtttcggg agtggaagca ggatcggcca gcgcacgaaa cgtggcagct
     7261 ctgtcggctg ccgaaacacc ggttgtgcat ccttccagct tatctacact cacggtgaat
     7321 ggtgttgaat gaatggatgt gttcacttcg gcctgcattt ccagttccag cgcatcgcat
     7381 ctttcttcgg taagcggtgc gcaaagcact ccacgaccgt ttttcagcat gaaattaatc
     7441 atttcaggtg taatcttttc ggcagcacag ataaagtcac cttcattttc acggtcttca
     7501 tcatctacca caattacaaa tttgcccatt cggatatcct caatggcatc ttcaatttta
     7561 tcaagttttt ctttcatagt tctttttttt gttgaatcac atcatctgcg tacgaatttt
     7621 tactttttct gaattaattc cgtgattttt ttcttctttt cctgtaatat tctttcatgt
     7681 tgtcataaat ccgtgtgtaa gattctgttc ggaagaagct tgcgtcggtc acagctttcc
     7741 aggtcagaaa caaacccagc gggaaaagta cggcagaact taaccacatg ccttcaaaaa
     7801 cctgccagaa accttcgcga gccattttat aaccggtggt atcaatgatg tagtatatta
     7861 caaacaacac caccgaaatc acgatgggaa atcccagtcc acctttgcgt ataatggcac
     7921 ccaggggtgc tccgataaaa aagaatatca gacaggcaaa tgacaaggta aacctcctgt
     7981 gccactcaat ctggtgacgg cgtaccgtca tggtgggatc cacaagcatt attctgttgt
     8041 aatcaatttt atccttcatt gcctgggcgc tctgacgggc agctgagact gcttcttcca
     8101 tttgctgctg gttgagtttc aggaaaagcg aatcggcagc gtaattcttc accggctctt
     8161 cttcggttac aacctgttct tcagatactt catcggtcat catcggaatt tcatcgatgc
     8221 ttacagtttc tctgatttcg ggaaactggg tggatatcat ttcgcgagcc tgctcatcgc
     8281 cccgtgcctt tacaattttc gacacagaat caatggtttg ggtaagttct accacatttt
     8341 tactcacatg ctggtctttc agtatcgact cgtcgtacct attgaattct gtatcaaaat
     8401 caagcagcag ctctttggtc tgaaatgttt cgcgacgata aggtatgctg cttgaattgt
     8461 atcttgagcc ttgctgcctc atgttttcaa aactttcgcc ggaatacatc gaaaatttca
     8521 ggtacttgtt atccgctgta aactgtactc gtccggaatc ggcaagggtt accgaagcat
     8581 tgtcaaatcc tttggagaaa tcataaatca tcagatccct gagcagtttt ttattcgggt
     8641 cctttgacct tacatacagg ttgtaaccat ttattccggt ataaaattct ccctcgggaa
     8701 tgtcaaattc gggtgatttc tgcctcaggg aaaaaattaa cgtccagagt ttttgttggg
     8761 ccatcgggag tacattgttc gaaaagaaaa atgccaggac actgattatc gagataaaaa
     8821 cgataaggcc ccgcatcatt ctgaaaagcg aaatgccggc cgatttcatg gcagtaagct
     8881 caaagtgttc gcccagatta ccaaaattca tcaatgcagc aagcaaaata gctaatggta
     8941 aagccatagg cacagtttgt aaggttgcat accagaaaaa ttcggccaaa actccccaac
     9001 ttaccccttt gcctatcaat tcagccatgt gcttccacgt aaactgcata agcagaatga
     9061 gcatggagat gaagaaagtc atgaaaaaat aagccagaaa tgactttaca atgtatgtat
     9121 caattttctt aattccgagc ataaatgaag ttatttttcg caaagcaaaa atactcaaat
     9181 ttcgacagac taccgtacat attttacaag atttatatga atgcagaatt ttgtctcttt
     9241 ttgaatttcc ggatgaaaaa caccagcgta gatgttgtga cgattactga taatgaatcg
     9301 gctacgggca tagagctcca cacaccatcc agaccgaaaa tgggcggcaa aaatatcaga
     9361 cacggaataa ggaaaagcac ctgacgggtc aaactcagtg tcatggatat tttggctttg
     9421 ccgatggact gaaaaaagtt ggttattaca atttgggaac caatgagtgg aaaaacagta
     9481 accgaaattc gtaaggctct ggcagcaagt tctttcaatt cctcgtctct cgtaaacaaa
     9541 cttgccaggg catgaggaaa aaatgttcct aaaataaatc ctgctgtaga aaccagggtc
     9601 gctgctacgg ctgttttttt caacgccaga aacattctgt cgaattttcg ggctccgtag
     9661 ttgaaaccga gaatgggctg tgcaccctga ttaagaccaa tgataaacat gacaacaagt
     9721 gtcgctatcg cattttgaat acccagagca cctactgcca aatctcctcc gtatctgatc
     9781 aaagcccggt tgatgatcac cactgtaaca cttgcagcta tctgcatact gaatggcgac
     9841 atcccgatgt tcagaattga ctttactatc tgccgatcca gtttcaggtt ctttctgtga
     9901 aaacgaatat ttgttttttt gtcgaaaaaa tgtaacacta cccatatcga accgacgaaa
     9961 aatgcaatat tggtggcatg tgcagcaccc gcaattccca ttttgaacac aaaaataaaa
    10021 agcggagcca gtgtgatatt gacaaaagcg cttataatca tggttatcat tgctttcttc
    10081 ggatagcccg atgctcgcat gatgctgttg aagccaaata acaaagcgga taaaatcgtt
    10141 cccggaacga tgatttgcat gaactctctg gcatatttca gggtattttc gctgcctccg
    10201 aacataatga gtatcggatt cataaataac atcgaaaaca gaattaccgt tccggaaacc
    10261 agtatggtca gagtaagtgt atttccgaga atctgttcgg cccggttacg gtcgccctcg
    10321 ccaagtcgta tagaaatgcg tgaagaagct ccggcaccaa ccagcatact gaatgccatc
    10381 agaaaattca taaaaggaaa cgtaagtgcc aatccggaaa tagccatagg acctactccc
    10441 agaccaatgt acatacggtc gatgatatta tagagcgaag acaccattgc tcccacaata
    10501 gcaggaagtg agtacatcca gagtagtttg cctacactca gggtacttaa tttactatct
    10561 tctgtttgct ttaattccga catttcgtat ttaagcattt ataaatgaat aatcttttcc
    10621 ggctaatttc acaaggattc atcaagttga tcctacaagg aaagaaaaat cggtttcact
    10681 tgctgtaaaa ccgaccgctt ttctgtttag tttccccgga tggattagaa ttcgtactca
    10741 aaagtgatgt accagccttt cattttttgc gaacggtttt tattctggct gatgttatta
    10801 attccccagt cgtatccgct ttttacacga taatttttcc attgtaaccc gccaccggct
    10861 cccagttgga tattcaggcg tcttaagtca ttatcatata aattgtaagt gccactcacg
    10921 ggataattta atgggttgga aggtgccgga tccagtactg tggctgtaaa tttaacgtct
    10981 tctgtttgtg ctaatccgat attgaaatca ggaccggcaa aagcaaacaa cttcatcccc
    11041 cagaaaatcg gcaaggtata ggtaatacgg agcggaacat ctatataatg acctgagtaa
    11101 ttgaaaaatg ccgtatcact gtgcgcatag atttgttcgc gattaccaaa agcaaacgaa
    11161 taccggagtc ctgtttccac tccaaagccc catttcaccg gaaattcaac attcactcca
    11221 ccttttagca catgataagg agatgtaacg atataatctc catatctgaa attctgacca
    11281 tatccggctt cagccctgaa cacaggaccc gagttttctg atttgaccgt atcttttttt
    11341 tctgtctgag caggcaggtt catcatcatt acagaaaaaa acaaaaaaac aacaattcta
    11401 aacttcatat tgtgaaattc ttttttttga atagaaactg gaataaattt ttgcaaaagt
    11461 agtgaaaata gctgcataaa cgctatttca aaacctttat taacattggt gttttacgga
    11521 aaatattatt ttcagaatgc ctactgatgt gtaataattg atatttgctc ccatttagca
    11581 gctacccggc gaactctaaa acgtgtaacc cactgatact ttcagatcgt ttgaccgtaa
    11641 tttagcgttg tcgagcgttg tgcggttcaa gataccccag tcatagcctc ctctcagaaa
    11701 taagcccatg aactgtacgc caatgcctgc acccatctga aaatccaacg aattgaaatc
    11761 gctcggcgcc tgatatatat ttgtaccttt tttgctcaga ttgacattga gtttattatc
    11821 agaatatgcc tcgtaatcag taaccttatt gatcgaatag ttgaagttag gtccggcaaa
    11881 aatctgcagg tagaattcat ctgcaagtgg cattgaatac attattttca gaggtaaatc
    11941 aaaggcagtg agtttgattc ggttctgatt ccagactccg gtttttttta aggctgaaga
    12001 acttaataaa tccactccgc tgaaataatt aaaaagcaat ccggtttgaa gcccaacctg
    12061 ctcactgaag ttgaatttca ggacaggccc ggcatgaaat ccattcaggt tgctcgaatt
    12121 gatggccgga tttttgatac ttcttgaatc cttgagtttt ggcattgtat agccatatcc
    12181 tgccataatg ccgaattcaa cttttttatc ctgagcgtaa gcgttaaatc caagtaatgc
    12241 cgttaacact accggtacta aaaatttgat aagtctcata tgtttttttt atgttttcga
    12301 atgcaaagat acataattaa cagctttcaa taaaacagtt gtttatctat ttaaaaataa
    12361 aaccagccgc gataacacat ccggctgatt tcatttttaa aaattcagtt ttatatttct
    12421 taaaaatggt aagcgatacc cactttcata tcgttgtttt tccagattgc atcagaatta
    12481 tttctgtcga gcattcccca gtcgtagctg aagcgcacag aaagctgatt gaacttgaga
    12541 cctgcacctg cacccaactg aagatcaaag ggagaataat gttttttacc gtttgacatt
    12601 tctattttat agatattgtt tgtttccaaa cttcccaatc cactttcaac ttttgttgac
    12661 tgagataatg catagttaaa attcggacct ccgaacacaa atgcacttac tgtacctccc
    12721 aacggaaaat caactgctaa acgtacaggt aaatcgagcc tgtgagccgt agtacttgaa
    12781 ttagctattc cccagtaatc attatttgtg gttaaatagt tgtacaataa accatactga
    12841 aggctcacag ctccctgaat tggcaaattg taaaccaaac cggcatgaaa tccattcaag
    12901 gcagcatcgg aatttgcatc ttttgagttg ctgtatccgg cctgaatacc taactgagca
    12961 tttgcaaaac caattgataa catggcagct aataatacga aagagatttt tctacttgtt
    13021 ttcataattt ttcttattta gttgaattaa aaaaatatat ggatggtaaa gtccaaaaat
    13081 cgtgccaacc agcttttttt ttaacttcta acaggtctgc gaacactctt tgcggcttct
    13141 tttactgaga atcaaacaat aaaaaataaa aaaccgggca aaatctttag aatcgaattc
    13201 tatctgattt tgcccgattt gtttgtttat tttaatccaa tcttctgaat ttcagaacag
    13261 attaatggtt gaaattttat tttgtaattt caaattctac gcgacggtta gcctgacgac
    13321 ctgcaactgt agcattgtca gcaattggtt ttgattcgcc aaagccatta gtaagcatgg
    13381 cagccattga taaacctttg gaagccaggt aatctctaac agaatttgca cgtttttcgg
    13441 acaaggtctg attgtatgca tccggaccta cgttgtctgt gtgaccgttg atgttcagtg
    13501 tgctccatgt ttgattgtct ctcaaaattg cagctacatt atcaaggata actaatgatg
    13561 catcggtaag ttttgctgaa ttgaaatcga attcgatatt gtcaaaagat gcgctgatca
    13621 ttgaaccttc ttctttgtct ttcaatcctt taacagcatc ttccagattt ttcagacggt
    13681 tctgaagatc cataccggct ttgtcaagtc catccagacg gctgttgata gcagatgggt
    13741 caaatgcatt ttcaacatgt tgaataacag gagctggtgc agggtagaag tcggcagtag
    13801 tcatatcacg tacgtgagtt ttgcttccgg ttccaagttt gaaacgtaaa gtagccatca
    13861 gcgtgttagc atcgttgtat tcaactacat ttgttaacgg tgtaatgtaa ccgcggtagg
    13921 tgtaatccaa accaagagct acaactctgc ctaaattata ttccattgaa gctcctgtgt
    13981 aagccagtgc attggttttg gtaggcatag ctgacattcc tcttgcataa gttgtggtcg
    14041 gagcaacata gtcagaatag ctcggcagac ttactccacc accaaagtta gcgtagaagt
    14101 tgagttttgc actgttacgt ttcgggaaaa gcaggtttga aagatttact gaagtgaaga
    14161 aactaggatc aatggtttta cctttgattg ttgagcggtt gaagttcaga tagtccagat
    14221 taagtccaac acctacaatc gggttgatag tgtattcgag ggcagcaccg gccccccagc
    14281 ttgcattgtc aataaaatcg tcgcctgcgg gattgatgtt gaaataatca acgccacctt
    14341 taagagcaaa agaccaatga gctgcatcag tttgagcaga tgtgtaagta atagcacttg
    14401 ctattatcat tacggttaaa aaaagttttt tcattttttt tgttttttat tagcagttaa
    14461 aataaattat gatgtgttgt tttcaaataa ttcacaaaaa tacgaataag ttttaactta
    14521 aattcatttt tgaataattt ttttaatcaa aagtgaacta aaggaacaaa attgatataa
    14581 atttccgttt gtaaaagtcc attttgatga aactttaaga aaataagata tttgtatcag
    14641 ctctaagatt agaataacga aaataatgcc cgaataattt gaaaaatgca gagaaaatct
    14701 ctctttcaga ttttattctg gttacgtatc gttacaacag aaacaaatga acttaagaaa
    14761 tgttttcatt tgttgtcaga aattcaacag aaagtggagc tggagggagt cgaaccctcg
    14821 tccaaacaag gaaacaatat gctttctaca tgcttatctt tgctttgatt ttcgtgctgc
    14881 agcaagacca aagctaccaa ctgctgcctt atcctttgtt atttcgctgc ccgtacaagg
    14941 ctacgaccag ctatccccga tttatccgca cctctgtatc aaatcgcctc ggggagaggg
    15001 catttgagag atgtctcgtc ctgatacctt gtatctggat taagtttaat ctactttatt
    15061 cgattaagca gcaagagcgt aattattttc gccagttatt gttttgaaat ccgggattaa
    15121 cgagccggca ttccgacgct cgacatgctt acataccttt tctgcctgct gtcaaaacca
    15181 aacagcccca aaatcgcttt tttggaataa ataataaatt aacaattctt atgccaaaat
    15241 ctcaaagatt ataacaattt taaaaaaaat atgttcaaca caagctgaac atatttttac
    15301 ttttacgctt tatatcattg tatgtaaact acttagcata gcttactgca cgtgtctcgc
    15361 ggataacggt aattttgacc tgtcccggat atgtcatttc gtcctgaatt tttttggcaa
    15421 tgtcaaatga caaaagttcc gtttctttat catccagttt ttcggccccg acaatcacac
    15481 gcaactctcg tccggcctgt attgcatacg ttttgattac tccgggatac gagagtgcca
    15541 gattttcgag gtcattcagc cttttgatat atgcttctac tatttcgcgg cgggcacccg
    15601 ggcgggcacc tgatatggca tcgcacacct gaacgatggg tgccagcata gtctccattt
    15661 ccacttcatc gtggtgagag ccaatagcat tgcatatttc gggcttttcc ttgtattttt
    15721 ctgccaatcg cataccgagc agtgcgtgtg gcaattcggg ttcatcgtcg ggcacttttc
    15781 cgatatcatg aagcagtcct gcgcgttttg cctttttcgg attgaggcct aattcagatg
    15841 ccatagtggc acacagattg gctacttcgc gcgagtgttg aagcagattc tgtccgtacg
    15901 acgaacggta tttcattttt cctaccattc ggatcaactc cggatgcaga ccgtggatac
    15961 ccaaatcgat ggtagttcgt ttgccggttt caatgatttc ttcttctacc tgcttgcgta
    16021 ccttatttac tatttcctcg atgcgggcag ggtgaatgcg tccgtcggta acgagctggt
    16081 ggagcgaaag gcgtgctatt tcgcgtctta ccggatcgaa tgccgagagt acaatggctt
    16141 cgggggtatc atccactatt atttcaacac cggtagcagc ttcgagtgcg cgtatattgc
    16201 gtccttcacg cccgataatg cgtcctttca cctcatcgga atcgatgtga aatacggtga
    16261 ctgagttttc gatggcagtt tcggttgcta cgcgctggat ggtttgtaca acaatttttt
    16321 tggcttcctt attggctgtc attttggctt catccattat ttcgttgatg tatgacagtg
    16381 cattggtttt agcttcctct ttgagtgttt ctaccagcct ttctttggct tgttcggccg
    16441 aaagccccga gatggtttcc agttgttcct tagcctgacg attgaggtgt tctgtttctc
    16501 gttttttgtt ttcgagggcg gtaagttgtt gttcgatgtt ttcggatttt gcatccactt
    16561 cgttcatttt gcgctgaagt tcgctctggc gctgactgag ttgcatctcg cgctgctgta
    16621 ttttcacttc ggctgcctgt accttggcat ttctttgctg tacctgctgc tcatgttcgg
    16681 ctttgagagc tatgaatctc tctttggcct ctatttcctt tttctttttc agcagttcgg
    16741 cttcagcctc cagctctctt ttggctctct tattgaatac tccgagcata aggtatgcgc
    16801 cgagaaatcc gagggcaaga gctactactg ctattattac gtagtaaatc atatttgttt
    16861 ttttgtttta taagtagttt attttaaaaa taaaaatcgc atcacattgt taacaaatct
    16921 ccagcaagat atcttcctga aaatgaatgc acaatctaat gcggtaatct gaataaaaat
    16981 gcattgaaag agagtaagta ctagctcatc aggcggatca actccttgtt gagttcctct
    17041 attttctcag ccagtggagc ctgatcctgg tttgttgcct gttttgcaac aatcaccgcc
    17101 agctgatatg ccgtcagtgt aagaatttcc tccatgcttc gctggctgaa ttgctttctg
    17161 tattgttcga gatacttatt tactatcttt tcggcgtttc tgtatatttc ttcgtcttcg
    17221 cgacgtatgt tcaacggcat tctgactccg ttgagtaata tatttattgt aaaagtttta
    17281 ttatcttcca tacgtttatt cgcttagaag cgccaaacat ttatcaattt cccgcactat
    17341 tttgttcaag tgctgacggg agttacggcg attttcatca gaaccgccta atccgtttgc
    17401 tgtttgtaaa agtttgtatt ctttttgcaa atccaggatt tctttgtgag cttccatcaa
    17461 atctctcttt tttccgtcca attcggctgc aagggtagca ttcttttccc gaagagcttt
    17521 ttgttcagaa atcagttttc gtatattcga ttcaaactcc ctgatgagat ctgcatatcg
    17581 gttttccata ctttttacta aatctccaca aaaataaggt tttgctttga ataaaaaaaa
    17641 caatttgctt aattttaatt caaatgaaga taaataagaa ctattccttt aacaattgtt
    17701 ttgatttttc gagtgcttca atgatgttgt tgcagatatt ttcgcttcct ataagtttat
    17761 caatttccac cttttgaagt acttcacgca cgttgtcgtt cacacccgaa agtacaattc
    17821 tggttttatc tttgtgggac atttttatca ggttttcaag attgtgagca cctgtagaat
    17881 cgataaaagg cactttacgc attcggataa ttctcacttt cgacgatccg cccacctgtt
    17941 tcatcgtttc ttcaaactta ttggctactc caaagaaaaa cggaccttcg atttcataaa
    18001 tcgatacacc tttcggtatt tcgagtttct ccgattcgat accttcttct acatagtccg
    18061 ttttatccac cacatcctga ataacagaca cggtagatac ttccgaaatt cttctcagga
    18121 aaagcaacac agcgagcagc agaccgactt caatggcaat ggtaaggtca aatatcacag
    18181 taagcagaaa tgtggcaagg agtacggtaa cgtctgattt tgtgtttcgc atcagcgatt
    18241 tgaaagtacg ccattcgctc atgttgtagg ctaccaccat taaaactccg gccaggcaag
    18301 ccatcggaat atgtttggtg agtccgccaa gaaagaacag aataagcccc agcacaatgg
    18361 cgtgaataat gcctgctacg ggagttctgc cgccgttgtt gatgttggtc atggtgcggg
    18421 caatggctcc ggttgccgga atgcccccga atatgggagt gattatgtta gccacgccct
    18481 gcgctataag ctcggtattt gaattgtgtt tgctgccgat aaccccatct gatacagtgg
    18541 ctgaaagcaa tgactcgatg gcaccgagca tggcaatagt aaaagccaca ggcaataatg
    18601 tacgcaccgt atcgaaatcg atgttgtaag tattggcctg aggaatggat gcattgatgg
    18661 taaagcgatc tccaatggtc tcaataccct gaattccaaa actatttttc agaaaataga
    18721 ccagcacggt cattaaaatg atggcaagaa gcgaaccggg aatttttttc gaaaaccggg
    18781 gagtgacact gatgataact attgaaagca aacctataag gagagtccac cagttaacag
    18841 aggatatatt ctgagcataa accacccatt tattcaggaa gtctgccggc actttttcaa
    18901 tctgcaaacc aaaaagatct ttgatctgag tggcaaaaat agttaaggct ataccacttg
    18961 taaaacctac cacaacggga aaagggataa acttaatgat ggtgcccagt ttaagcacac
    19021 ccattgccac cagcatcagg ccggctataa tggtggcgat agccagtcct gtcaccccgt
    19081 actgctcaat tattccatac actattacga tgaatgctcc cgtagggccg ccaatctgaa
    19141 cacgactgcc cccgagcaac gatatgatga aaccggcaat aatagcggta aaaatgcctt
    19201 tctccggcga tacacccgaa gcaataccaa atgcgatagc cagtggcaaa gctacaatac
    19261 caacaataat accggccatg aggtcggaat
//
LOCUS       DAORKP010000024        19239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_143_length_19239_cov_7.945423, whole genome shotgun sequence.
ACCESSION   DAORKP010000024 DAORKP010000000
VERSION     DAORKP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19239
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_19239_cov_7.945423"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1053
                     /locus_tag="PLN75_02580"
     CDS             163..1053
                     /locus_tag="PLN75_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRG03034.1"
                     /translation="MNYFQSHIGEIAALLVSFTWTGSALFFEKAGKQIGSLQVNIIRL
                     FLAVFFLGFTTLITRGMFFPMDATAYNWFWLAISGVVGFFIGDLMLFKSYSVIGSRTA
                     SLIMSLHPMMTAIIGWFFLNEVLSPRSIAAILISLTGIIIVIFNRNMKLNIPFKGFVL
                     AFLAALGQAVGLILSKKGIGDYDPMAATQIRAIFGFVCFALFITVLGRWGKVKSAVQN
                     REGMKAVSIGTVFGPFVGVSLSLFAVQHTQTGIASTLMALVPILIIFPSSLMFKEKIT
                     TRQVVGAVISFIGVSLFFTR"
     gene            complement(1046..1930)
                     /gene="dapA"
                     /locus_tag="PLN75_02585"
     CDS             complement(1046..1930)
                     /gene="dapA"
                     /locus_tag="PLN75_02585"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRG03035.1"
                     /translation="MLNSKLVGMGVALITPFKEDESIDFDALTKVIEHQIKNGTDYLV
                     VCGTTAETPTLTGEEKEEIKQFVAQTAAGRIPLVYGIGGNNTKEVVNKIQTTDLTGYE
                     AILSVTPYYNKPSQEGLYQHYAAIAKASPLPIILYNVPGRTGVNMTAETTLRIANEFK
                     NVCAVKEASGNFSQIDDIIKNKPEDFMVISGDDGITFPLITLGAVGVISVIGNAFPKE
                     FSRMVRLALEGDYRSARQIHHKFTELISLLFVEGNPAGVKSMLAIMGLIENKLRLPLV
                     PNTIKTYEKIRRVLNEII"
     gene            complement(2040..2651)
                     /locus_tag="PLN75_02590"
     CDS             complement(2040..2651)
                     /locus_tag="PLN75_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03036.1"
                     /translation="MKKLNQYILKRRVAKFLKETNRAKRYVDYKNASTILLIFESDYI
                     EKNRFIRKAIEVLTADGKKVFAWGFLDKKMTSTAILPTFKILDRSTIDWFGCPKTPYL
                     KELMEVQYDMVIDLTLTDIIPLQYVCLYSNASMKSGMSRSMDDVLDFVIKIPQPEPQD
                     IPENEEERRKIEFQDLNESLFHTDQQYLFEQLIFYLKRIKTND"
     gene            complement(2729..4744)
                     /gene="ligA"
                     /locus_tag="PLN75_02595"
     CDS             complement(2729..4744)
                     /gene="ligA"
                     /locus_tag="PLN75_02595"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRG03037.1"
                     /translation="MTDLRNQINSLRHELEQHNYNYYVLNNPTISDFEFDMKMKQLEE
                     LEKEHPEFFDPNSPTQRVGSDVISSFKQVKHQYPMLSLSNTYSEGEVNEFFERTRKAL
                     NTGFELVCELKYDGTSISLVYENGLLVRAVTRGDGEKGDDVTSNVKTIRSIPLRLQGN
                     DFPEKFEIRGEVLMPWNVFDELNKEREEQEEPLFANPRNAASGTLKMQNPAEVAKRKL
                     DGFFYFLLGDVLPSDSHFDNLQAARKWGFKVSDAIRRCKNLQEVIEFINYWDAERKNL
                     LVATDGIVIKVDSLSQQRNLGTTAKSPRWAIAYKFQAEQATTRLNSVSYQVGRTGAIT
                     PVANLDPVQLSGTTVKRASLHNADIIESLDLHIGDRVYVEKGGEIIPKITGVDKEARI
                     LIGEKIRFISKCPECGTLLKRFEGEAAHYCPNENACPPQIKGKMVHFVSRKAMNIDGL
                     GEETINLLYQNELLRNIADIYELKASDIARLERLGEKSAKNIINGIEISRNVPFERVL
                     FALGIRFVGETVAKKLALAFQNIENLEKATIEELTNVDEIGQRIAESVIAYFDDSLNR
                     EMVEKLKMYGLKMSVSEEKLRNMSDTLSGKVIVISGTFAKHPRDEYKTIIEQHGGKNS
                     GSVSSKTSFILAGENMGPEKLNKAQKLNIPVITEDEFLKMIDLKETI"
     gene            complement(4952..6196)
                     /locus_tag="PLN75_02600"
     CDS             complement(4952..6196)
                     /locus_tag="PLN75_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG03038.1"
                     /translation="MNLEYFIAKRIHFEKKGEKNVSRPAVRIATIGIAVGVAVMLIAI
                     AVVIGFKQEIRNKTIGFGSHIQITNFDNNNSYEMQPIRMSDTLQNQLKAIPNVRHIQR
                     FVTKPGIIKTENDFQGMILKGVGPEFDWSFFKSNLIEGEILNLNDSTPVNEAIISKSI
                     ADMMGLKLGDSFLTYFLQDQIRARKFTVKGIYSTNFADYDKLFVITDIRIAQRLNGWD
                     QEYFSGMEILITDFNYLDGVSDQVYALAANRFDAEGNGYFIQTIRQLNPQIFSWLDLL
                     DMNVWVILLLMLAVAGFNMISGLLILILEKTNMIGILKSYGAGNWSIRKIFLYESIFL
                     IGKGMLWGNIIGLLICFVQYQFKIIPLDPVSYYTSTVPITFNWLYIVLLNAGTLIVSV
                     LMLIGPSYLITKISPAAIMRYE"
     gene            complement(6387..8273)
                     /locus_tag="PLN75_02605"
     CDS             complement(6387..8273)
                     /locus_tag="PLN75_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRG03039.1"
                     /translation="MKYKILLLIIILLSVNLSAQQTQKPELHGKKWMAVTGKSLGATA
                     GAMIFSKGGNAVDAACGMLAATCTLWDVLSWGGETQALIYNPKTKKVIAINALGVAPT
                     GATVEFFKSRGYTFPPEYGPLAAVTPGTFGGLCYMLSNYGSMSLKEVLAPAMVLAEGF
                     PVDIPFAKSVARNKEKLTQWKYSKKLFITGNENNTETPQPGEIFVQKDLLNTLKKLVE
                     TEQKALRQGKKRKDAIMAAYDRFYKGDIAKEFVRSVQEQGGLITEKDLATWKPVEEEP
                     LHTTYRGIEVYKLNAWTQGPVMLQALNILENFDLKALKYNSADYIHTLYQTMNLVFAD
                     RDFYYGDPYFEQNIPMKGLLSKEYAKKRAEKIRNIKLNTSSEPGNPYLYEGKTNPYLH
                     LLKLRDFEHNFNNNRDFSPKHDVVNQNYTDNEYTDRLWRGTTTIEAADEEGWVVSVTP
                     SGGWIPACIAGETGIGLSQRMQSFVLDSMLNPHNVVKPGKRPRVTLSPTIALKNGKPL
                     LSFAVQGGDTQDQNQLQFFLNMVEFGMNVQQACEAENFNSNQLWLSLGGTKTEDRAPR
                     TGEILLNIQTPDSVRSELIKKGYKIQVANRTSGPLNGIYFDEKFNTFWGGSSNYGEDY
                     GIGW"
     gene            complement(8464..9657)
                     /locus_tag="PLN75_02610"
     CDS             complement(8464..9657)
                     /locus_tag="PLN75_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRG03040.1"
                     /translation="MPNTSQRGQSMPESPIRKLAPLAEKAKARGVKVYHLNIGQPDIK
                     TPKVALDAIRNIDREILEYSPSDGFKSLRLKLAEYYRSFNIDVAFDDIIITTGGSEAV
                     SFAFMSCLDPGDEIIVPEPAYANYTAFAIGAGAVVRPVVSSIDDGFALPPIEQFEELI
                     TPRTKGILICNPNNPTGYLYTRKEMNLIRDLVKKYDLYLFSDEVYREFCYTGAPYISA
                     FHLDSIEDNVILIDSVSKRYSECGIRIGALVTKNKDVRKTVMKFCQARLSPPLIGQIA
                     AEASMGTPKEYMMEVYDEYVERRKFLIDGLNRIPGVYSPIPMGAFYTTARLPIDDSDK
                     FCAWCLSDFEYEGCTVMMAPATGFYTEDGLGKNEVRIAYVLNKEDLKHALVVLGKALE
                     AYNAK"
     gene            complement(9799..12258)
                     /locus_tag="PLN75_02615"
     CDS             complement(9799..12258)
                     /locus_tag="PLN75_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG03041.1"
                     /translation="MRYKIIFILFFLLSVASVFAQGQKVRIYGYVIDENNRGIEDAGV
                     RFEGMNNGTSTNRNGYYDLYVNITDSVKIVYSHLGYETIRHTIFPGKQVVQITVALQA
                     LSRELKTVDVTVNRRSTSNMQTLEAEKYKLMPNASGSFESLLISFAGVTSNNELSSQY
                     NVRGGNFDENIVYVNGTEIYRPLLIRAGQQEGLSFINPDMVGNVAFSSGGFNAEFGDK
                     MSSVLDVKYKKPISFEASGAISLLGASAYLGTAGNRFTQMHGIRYKTSKYLLGTLDTK
                     GSYKPDFIDYQTYLTYQISPRVEINFLGNFSRNNYTFIPDSQSTAFGTFNMAEKLNVY
                     YEGQEKDLFQTAFGALSLLFRPKENLKLSITTSTFSTNENETYDITGNYVLGELKMGD
                     TGETEEGEKLGVGTYHEHARNRLKATVANISHSGEYIRSGHTLKWGVNLQREIISDKI
                     NEWEWRDSAGYSMPYTDDKVVDLFYNMKSTQKLDSRRTSAYIQDTYRWDTEHALYALT
                     GGLRASHWSYNREFLLSPRASVSILPHWDKDFNFRLATGLYYQSPFYKELRDTVSDAL
                     GNVTVHLNPNLKAQRSLHLVLGGDHYFRWLGRPFKFTTEAYLKLADRVTTYSVDNVRI
                     RYSGQNDAKAYTAGVDFKLFGELVPGTDSWINFSLMRSREDVLNDFYTDKEGNKVYPG
                     WISRPNEARYSLSMLLSDYLPNNPKYKLYLKTIFSDGLPVGPPRSPRYMADRFRLRDY
                     KRVDIGASRVIVNGSDRIMNKRWMKNIASIWLNLEVFNLFDFKNENSIYWVSDIYGYQ
                     HGSPNYLTGRQFNLKLMVDIK"
     gene            12558..13337
                     /locus_tag="PLN75_02620"
     CDS             12558..13337
                     /locus_tag="PLN75_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006846441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRG03042.1"
                     /translation="MTGNSEGIFQRTELLLGNDFLRCAAEKRVILFGVGGVGSWCAET
                     LIRSGIVHLTLVDSDRVSMTNVNRQLMATTKTVGLVKVEVLKQRLLEINPTAEIIALQ
                     RIYSPETHETFMLESYDFIIDAIDSLANKVYLIQQATKTNARFFSSMGAALKVDPTRI
                     RVDEFWKVQGCPLAKALRSRLKKTGGVAKKFTCVYSDELLENKGDMPTGQVENNPEAY
                     DNEGSIWDSRKARINGTSSYMPAMFGMTITSLVVKAIMNEK"
     gene            complement(13351..14040)
                     /locus_tag="PLN75_02625"
     CDS             complement(13351..14040)
                     /locus_tag="PLN75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRG03043.1"
                     /translation="MKFIIIGLGHFGASLGERLTNMGHEVIGVDKNMDKVDDLKDKLT
                     HVVCLDSTDSAAINTLPIANTDIFIVCIGENEGENLMTTALLKKMQVKRLISRSVSTL
                     HENVLEAMGITEIVRPEIETADRWALKLSNANFVNLFEITKEYNIVELVVPAKMIGRT
                     VMNINFNKIYNVIVLTKLKQVSEINKLGVSRTIIKAGEIVTANTRIEENDILVVYGHR
                     DDIKRMLRENE"
     gene            complement(14056..15825)
                     /locus_tag="PLN75_02630"
     CDS             complement(14056..15825)
                     /locus_tag="PLN75_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRG03044.1"
                     /translation="MAEKPNAYRNLLGINKASFTLAVIAFILSVVDLGFRLEYRTDQF
                     FLFIYHLAIALCLTSLLARIYIKRNNLQHKIVRIDMILSIFLIFVLIHNLARLFTFGF
                     HSMHNSWTYIALFLVFLREVYNINFRFRYKLINPAQLFVASFFIIILMGSLLLMLPNA
                     TRGDISYVDALFTATSAVCVTGLTVVDTGSVFTLIGQTIIIALIQIGGLGIMTFTSYF
                     SYFFRSGATYENQMYMGQMSNVDKLDDVFNVLKKIILVTFSIEASGAVFLYSSISNDV
                     VMGVGQRIYFSVFHSVSAFCNAGFSPLKNNLFEITFRYNYFFQLVIALLIITGGIGFP
                     IIFNVWKYFIYKIGNSFKRLVRKREKTYLPWVLNLNSKVIITTTAILLISGTVFIFLL
                     EYNNTLTEHRSFFGKLVVSFFGSVTTRTAGFNSVDTAAMTLPVTLIFMLLMWIGASPA
                     STGGGIKTSTIAVACMNVISIARGKKSLEIFGREISQVTINKSSAIILLSFAVIFVSS
                     ISVSAFDSDKSLLSIVFEVVSAYGTVGLSRGITGSLTDASKIIIVITMFLGRVGMITF
                     LMAIIKKSVNTKYRYPSEEILIN"
     gene            complement(15970..18147)
                     /locus_tag="PLN75_02635"
     CDS             complement(15970..18147)
                     /locus_tag="PLN75_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRG03045.1"
                     /translation="MNAENIRLYRNLYTSHSTLLTHSERRELSRILKHFLSEKKYGVE
                     NPEAFLKRNLEIMDILLNEIGLGKAAVISFLLMEPVEKQHIDVKDVDKLFSTSISPIM
                     IGLQRVKELYQKNVALESENFRKLLLTMAEDVRVILIIVAVRLQTMRNLESYPTDEQQ
                     QIARESSFLYAPLAHRMGLYKVKTELEDLSLKFTDREIYKAIARKLNETKRSRDAYMN
                     AFIEPLKKEMEEIGFPFEIKGRTKSIHSIYSKMQKQNTTFENIYDLFAIRIIFDTPIE
                     KEKAACWQAYSIVADMYQPNPKRLRDWLSIPKSNGYESLHTTVLGPQNKWVEVQIRTA
                     RMNEVAEMGLAAHWKYKGIKSESGMDEWLKNIREILENPDLNAVDFMDEFKLNLYDKE
                     VFVFTPNGDLHKLPKDATLLDFAFSIHTNLGTQCVGGKVNGKHVQIKYVLNNGDQVEV
                     LTSPTQKPNPAWLNIVTTSKAKNKIRQSLKEVEFKDAEIGRETLIRRFKNWKIELEDG
                     RFSRISKKMGYKTVSDLYQAIAHEKVDIHEVRELYLELETRDNTTATENRTAENFVKE
                     VAAIPETASKEDVLVIDNNLRNVDYKLAKCCNPIYGDEIFGFVSVMGGIKIHRTNCPN
                     APQMISRFGYRIIKARWSGKSEGSQYPITLRVVGHDDIGIVTNITSIISKEKEVMLRS
                     ISIDTNAGLFQGNLTVMVNDNKSLDDLIKKVKNVKGVKNVSRT"
     gene            18340..19059
                     /locus_tag="PLN75_02640"
     CDS             18340..19059
                     /locus_tag="PLN75_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRG03046.1"
                     /translation="MPENQFISNSLENNLLISIRSDSILYSVTDENEQVLLRQEINML
                     PGKMADATVYGHFFNQPELNILSENVLILIENSHFQLIPDELFREENMRELFELEFGK
                     MENSKIKYILLPKWGAHLVFGAPAAMIDFFEAKYPDAVIEHHISGLLKKKINKSDEAV
                     FAYLRKDTVDVLVVKDNLLQLVNSFDVKTTEDCCYFVLNVYEQLQLNTESMTLVLMSE
                     NSVNEEFIDLLKKYIVTVKVK"
     gene            19061..>19239
                     /locus_tag="PLN75_02645"
     CDS             19061..>19239
                     /locus_tag="PLN75_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRG03047.1"
                     /translation="MRIIGGKYKAKRISVPHNITARPTTDFAKEGLFNLLNNRIDFED
                     IDVLDLFAGTGSIGF"
BASE COUNT         5091 a         4055 c         4086 g         6007 t
ORIGIN      
        1 aagtagctca gtcgatagag cattagcctt ccaagctgag ggtcgcgggt ttgagccccg
       61 tcttccgctc tcgatgaaag gagagatatt aactgattgt tgatgtctct ccttttttga
      121 aattatcaaa aagtggtatt ttgaaataat aagcattcaa atttgaatta ctttcagtca
      181 catataggcg aaatagctgc gttactggtt tcatttacgt ggactggcag cgccttgttt
      241 tttgagaaag ccggcaaaca gataggttcg ttgcaggtaa atattatacg tctttttctg
      301 gcagtttttt ttctgggatt tactaccctg ataaccagag gcatgttttt cccaatggac
      361 gctactgctt acaactggtt ttggctggcc atttcgggtg tagtcggctt ttttatcggc
      421 gatttgatgt tgttcaagtc gtattctgta atcggatcgc gtactgcatc gctcatcatg
      481 agtcttcatc cgatgatgac agctataata ggatggttct tcttgaatga agtgctatca
      541 cccagaagca ttgctgccat tcttatcagt ctgaccggaa tcattatagt catattcaac
      601 aggaatatga aactgaatat cccgttcaaa ggttttgttt tggcattcct ggctgctctc
      661 ggacaagccg tagggctgat actgagcaaa aagggaattg gtgactacga tccgatggct
      721 gccactcaaa tcagggcgat ttttggtttt gtgtgttttg ctctgtttat taccgtatta
      781 ggtcgttggg gaaaggtgaa atcggcggtt caaaacaggg aaggaatgaa agctgtatcc
      841 ataggaacgg tttttggtcc gtttgtgggt gtatcgttgt ctttatttgc agttcagcat
      901 acacaaacag gtattgcatc cacattgatg gcactcgtac ccattctgat tattttcccg
      961 tcatcgttaa tgtttaaaga aaaaatcacg acccgccagg tagtgggagc cgtgattagt
     1021 tttattggtg taagcttatt ctttactaga taatttcatt caatacccgt cggatttttt
     1081 cgtaagtttt gatggtgttt ggcaccaaag gaagtcttaa tttgttctca atgagtccca
     1141 ttatggccag catgctcttt actcctgccg gatttccttc cacgaaaagc aagctgatca
     1201 gttctgtaaa tttgtgatga atttgccgtg cgcttctgta atcgccttcc agtgcgagcc
     1261 tgaccatgcg gctgaactct tttggaaaag cattcccgat taccgaaata actcctacgg
     1321 ctcccagtgt tataagcggg aatgtaatgc cgtcgtcgcc ggaaattacc ataaagtctt
     1381 cgggtttgtt cttgataatg tcgtcaatct gactgaaatt tccggatgct tcttttacag
     1441 cacaaacatt tttgaactca ttagctatgc gaagtgtggt ttctgcagtc atgtttacgc
     1501 ccgttcgccc gggtacgttg taaaggataa tgggtagggg agaggctttg gctattgctg
     1561 cataatgctg ataaagtcct tcctgcgatg gtttgttata gtaaggagtg acagaaagta
     1621 tagcctcgta tcctgtcagg tcagttgtct gaatcttgtt tacgacttct ttggtgttat
     1681 ttccaccaat tccgtaaacc aacggaattc ttccggctgc tgtttgagcc acaaactgtt
     1741 ttatttcttc tttttcttcg ccggtcagtg tgggtgtttc ggcagtagtg ccgcatacaa
     1801 caaggtaatc ggtaccgttt tttatctgat gttctatcac ttttgtaaga gcatcaaaat
     1861 caatggattc atcttctttg aagggagtaa tgagcgccac gcccattcca accagtttcg
     1921 agttcagcat gtttgaaata tcttgtttta ttacggtcag tttctgtttt gaataattaa
     1981 aaaaacttca atcggtagcc tctgcaagtg ttttgaaatt ttctgcaaaa ttacactttt
     2041 taatcgttag tttttattct tttgaggtaa aaaattaatt gttcgaaaag atattgctga
     2101 tcagtgtgaa aaaggctctc gttcaaatcc tggaattcaa tttttcttcg ttcttcttca
     2161 ttttcgggta tatcctgcgg ttccggttgg ggtattttaa ttacaaaatc cagcacatca
     2221 tccattgaac ggctcattcc gcttttcatg gatgcatttg agtaaaggca tacatattga
     2281 aggggtataa tatcggtgag agtcagatca attaccatgt cgtactgaac ttccatcagt
     2341 tctttcaggt aaggagtttt ggggcatcca aaccagtcga tagtgctgcg gtccaggatt
     2401 ttgaaagtag gcaggatagc ggttgaagtc atctttttat ccagaaaacc ccatgcaaaa
     2461 accttttttc catcggctgt gagcacttct attgctttgc ggatgaaacg gtttttttca
     2521 atataatcgc tttcgaaaat gagtaatatg gtacttgcat tcttgtagtc gacatatcgt
     2581 ttggcccggt ttgtctcctt taaaaatttg gcaactcttc tttttagaat atattgattg
     2641 agttttttca tttcaaaagc atgttcttct gcaaaagtaa caataattgt caaatacaca
     2701 tatcagggtt aaataaactt tacaaatttt agatagtttc tttcaaatca atcattttta
     2761 gaaattcatc ttcggttatt acgggaatat ttaatttctg tgctttattg agcttttctg
     2821 gtcccatatt ttctccggca aggatgaaac ttgttttgga tgatacgctg ccgctgtttt
     2881 ttcctccgtg ctgttcaatt attgttttgt actcgtcgcg cgggtgtttg gcaaatgtac
     2941 cgctgattac gattaccttt ccggagagtg tatcactcat gttccggagt ttttcttccg
     3001 aaaccgacat tttcagaccg tacattttta gcttttctac catttcccgg tttagcgaat
     3061 cgtcaaaata ggctatcacg ctttcagcta ttctttgtcc tatttcatcc acatttgtga
     3121 gttcttcgat ggtggctttt tccagatttt cgatgttctg aaatgccagt gcgagttttt
     3181 tggccactgt ttcgcccaca aagcgtatcc cgagtgcgaa aagcactcgt tcgaaaggga
     3241 catttcgtga aatttcgatg ccattgatga tgtttttggc tgacttttcg cccagacgtt
     3301 ccaggcgcgc tatatcggat gctttcagtt cataaatatc tgctatattt cttaataact
     3361 cattttgata tagcaggtta atggtttctt cgcctaagcc gtcgatattc attgctttgc
     3421 gactgacaaa atgtaccatt tttcctttta tctgcggagg acatgcgttc tcattggggc
     3481 agtagtgagc tgcttctcct tcaaaacgtt tcagcaaagt accgcattcg gggcattttg
     3541 aaataaaccg tattttttcg ccaatcagta ttcgggcttc tttatctaca cccgttattt
     3601 tcggaataat ttcacctcct ttttccacat aaaccctgtc accgatgtgt aaatcaaggc
     3661 tctcaatgat gtcggcattg tgtagcgaag ctcgtttcac ggttgtgccc gaaagttgaa
     3721 caggatccag attggcaacg ggcgtaattg cgccggtccg accaacctga taggacactg
     3781 aattcagcct cgtagtagcc tgctctgcct gaaatttgta tgcaatggcc catcgcggcg
     3841 atttggcagt agtacccagg ttgcgctgct gactgagcga atccactttt atcactattc
     3901 catcagtggc tacaagcagg ttttttcgtt ctgcatccca gtaattgata aattcgatga
     3961 cttcctgcag atttttgcat cgcctgatgg cgtctgatac tttgaatccc cattttctgg
     4021 cagcctgcag gttatcaaaa tgactatcgg acggcaaaac atcgcccagt agaaaataaa
     4081 aaaatccgtc taattttctt ttcgcaactt ctgccggatt ttgcattttc agcgtacccg
     4141 aagctgcgtt gcgcggattg gcaaaaagtg gttcttcctg ttcttcgcgt tctttattca
     4201 actcatcaaa cacattccag ggcataagca cctctccgcg tatttcaaat ttttccggaa
     4261 aatcgttgcc ctgcagcctg agcggaatgc tgcggatagt tttcacgttg gaagtcacat
     4321 catcgccttt ttcgccgtcg ccacgggtaa ccgcacgtac aagcagaccg ttttcatata
     4381 ccagcgaaat ggaggttcca tcgtatttta gttcgcagac aagttcgaaa ccggtgttca
     4441 atgcttttcg cgttcgttcg aagaattcat tcacttcgcc ttccgaatag gtgttgctga
     4501 gcgaaagcat cggatattga tgtttcactt gtttgaatga actgattaca tcacttccta
     4561 cccgctgagt gggcgaatta gggtcgaaaa attcgggatg ttccttttcc agttcttcca
     4621 gctgcttcat cttcatatca aattcaaaat cggaaatggt cggattgttc agcacataat
     4681 aattgtagtt gtgctgctca agttcatggc gcagagaatt gatctggttc cgtaaatctg
     4741 tcactccaac tccctgaagc ggggattttg gttcgtctga ttgattgtat atatctgaaa
     4801 aaaggtcttt cataaatatt acaaaataaa ttatcaaatc ttaataagtc agaaaaagtt
     4861 ttatattcct tccaatattc cccctttaga gcctgttcct gcctgtcccg attttatatc
     4921 gggaaatttg gttcgggagg gtcagaaggc ctcattcata cctcataatc gctgccggcg
     4981 aaatttttgt aatcagatat gaaggcccga tgagcattaa aacggacaca atgagggtgc
     5041 ccgcattcaa aagtacgatg taaagccagt tgaaagtaat aggtacggtg cttgtatagt
     5101 atgatacggg atcaagcggg atgattttga actgatactg aacaaagcaa atcagtaatc
     5161 cgataatgtt tccccaaagc attcctttac caatcagaaa aatggattca taaagaaata
     5221 ttttacggat tgaccagttg ccggcgccgt aagatttcag aattcctatc atgtttgttt
     5281 tttcaagaat gaggattagt aaaccggaaa tcatattgaa gccggcaact gccagcatga
     5341 gcagcagaat cacccatacg ttcatgtcca gcaggtccag ccagctgaat atctgcggat
     5401 tcagctgacg gatggtctga atgaagtagc cgttgccttc agcgtcaaac cgattggcag
     5461 caagcgcgta aacctgatcg cttactccgt caagatagtt gaagtcggta atgagtattt
     5521 ccattccgct gaaatattcc tgatcccagc cgttcagccg ttgtgcaatt cgtatatcag
     5581 tgatgacgaa cagtttatcg taatcggcaa agttggtgga atagattcct tttacggtga
     5641 attttctggc ccgaatctga tcctgcagaa aataggttaa aaaagaatcg cccagtttca
     5701 atcccatcat atcggctatg gatttggaga tgatggcctc atttaccggt gtggagtcgt
     5761 ttagattcag aatctcgcct tctataaggt ttgatttgaa gaaactccag tcgaattcag
     5821 gccccacacc tttcagaatc attccctgaa aatcattttc ggttttgata atgcccggtt
     5881 tggtcacaaa tcgctgaata tgtctaacat tcggaattgc tttcagttga ttttgcagtg
     5941 tatcactcat cctgatgggc tgcatttcgt aggaattatt gttgtcgaaa ttggtgattt
     6001 gaatgtgaga accgaatccg attgttttat tacggatttc ctgcttgaaa ccaataacca
     6061 ctgcaatggc tatcagcatc acagccacac ccacagctat tccgatggta gcaatacgca
     6121 cagcaggccg ggaaacattt ttctcaccct tcttttcgaa atggatccgt ttggctatga
     6181 aatattctaa attcaatgtt tgatgattag tcccgataga tcggcgattt ttatttattt
     6241 agcaaagaat tacaaagtta tgctttttta ttgattcagg gaggcagatt gtatcacaaa
     6301 agatgtattt ctgccttttt aaatatcgga aaatggcgat ttcagtccga ataattctga
     6361 aattcctgaa gaaatcttta acgttctcac cagcctattc cgtagtcttc gccgtaattg
     6421 ctgcttcctc cccaaaaagt attgaatttt tcgtcgaaat aaatgccatt cagcggtccg
     6481 ctggtgcggt ttgcaacctg tattttgtat ccttttttaa tcaattcact tcgaacagaa
     6541 tccggagttt gtatatttag taaaatttct ccggttcgtg gagcccgatc ctctgtttta
     6601 gtaccgccta acgaaagcca gagttgattg gaattgaaat tctcagcctc acatgcctgc
     6661 tgtacattca taccaaattc aaccatattc agaaaaaact gaagttggtt ttgatcttgc
     6721 gtgtcgccac cctgcacagc gaatgatagc agcggtttcc cgtttttcag tgctatggtg
     6781 ggtgaaagcg taacgcgggg tcgttttccg ggcttaacca cattgtgagg attcagcatg
     6841 gagtcaagca caaaactttg catccgctgg cttaatccaa tgcctgtttc acctgctatg
     6901 catgcaggaa tccaaccgcc tgacggagtg acagatacta cccatccttc ttcgtcggca
     6961 gcttcgatgg tggttgtgcc acgccataaa cggtctgtat attcattgtc agtataattc
     7021 tgatttacaa catcatgctt gggagaaaaa tcacgattat tgttaaaatt gtgttcaaaa
     7081 tcccgtaact tcaacaagtg aagataggga ttcgtttttc cttcgtataa atagggattt
     7141 cccggttcgg agcttgtatt taattttatg ttgcgtattt tttcggctct tttttttgca
     7201 tattctttgc tcaaaagtcc tttcatcggt atgttctgtt cgaaatatgg atctccgtaa
     7261 taaaagtctc tgtctgcaaa tacaagattc attgtttgat acaatgtgtg aatgtagtca
     7321 gcactgttat atttcaaagc tttcaaatca aaattctcca ggatgttcaa tgcctgcagc
     7381 attaccggac cctgagtcca ggcatttagt ttgtacactt ctattcctct gtaggttgtg
     7441 tgaagtggtt cttcttcaac aggtttccag gtggcgaggt ctttttcggt tatcaatccg
     7501 ccctgttcct gcacacttcg cacaaattct ttcgctatat cacccttata aaaccggtcg
     7561 tatgccgcca taatagcatc tttccgtttt ttcccctgcc tgagcgcttt ttgttctgtc
     7621 tccaccagtt ttttcagtgt gtttaacagg tctttctgaa caaaaatttc acccggttga
     7681 ggtgtttctg tgttattctc attcccggtg ataaacagtt ttttgctgta tttccattgg
     7741 gttagttttt ctttgttcct ggctacactt ttcgcaaaag gaatatctac cggaaagcct
     7801 tctgccagaa ccattgcggg agccaatacc tctttcagac tcatggagcc gtaattggaa
     7861 agcatataac acaatccgcc aaaagtcccg ggagttaccg ctgccagcgg cccgtactca
     7921 ggtggaaaag tataaccgcg acttttgaag aattccacag ttgcaccggt aggagcaaca
     7981 cccaatgcat ttatcgcaat aacttttttt gttttcggat tgtaaatcag tgcctgtgtt
     8041 tcgcctcccc aacttagtac atcccacagc gtgcaggtgg cagccagcat tccgcaagct
     8101 gcatcaacag catttcctcc tttgctgaaa atcattgcac cggcagtagc tcccagactt
     8161 ttgcctgtta cagccatcca tttttttccg tgaagttcgg gcttttgggt ttgttgagct
     8221 gataaattta cactaagaag gataattatc agaagtagta ttttgtattt cataagaaac
     8281 ttcctgatga acgttttaat aacattactg aattgaccgg acacaaaaat actaaaaatt
     8341 catataaaca aaaacacccg acttccgaat gaatatcatc aaaagtcaga tgcgtttagt
     8401 ttgataaaaa aaaacatgaa atttcaatat aaaaacaatc tgatgttttc caacggaaat
     8461 gagttatttg gcattgtacg cttccagcgc ttttccaagc actaccagag catgtttcag
     8521 gtcttcttta ttcagcacat aggctattct aacctcattt ttacctaaac cgtcttcagt
     8581 ataaaaaccg gttgccggcg ccatcatcac tgtacatcct tcgtactcaa aatccgaaag
     8641 acaccaggcg cagaatttat cggaatcatc aatgggcagt cgggctgtgg tatagaatgc
     8701 acccatcggg atgggagaat acacgcccgg aatgcggttc agaccatcta tgaggaattt
     8761 tcgtcgttcc acgtattcat catacacctc catcatgtat tctttgggcg tacccatgga
     8821 agcttcggct gcaatttgtc cgatgagtgg cggactcagc cgcgcctgac agaatttcat
     8881 caccgttttt cgtacatcct tgtttttggt aaccagtgcg ccgatacgta tcccgcattc
     8941 gctgtaacgt ttcgaaacgc tgtcaatcag aatcacgttg tcttcaatgc tgtccaggtg
     9001 gaaggctgat atatacggag ctccggtgta acagaattca cgatagactt catccgaaaa
     9061 aagatacaaa tcgtattttt tcaccagatc acgtatcaga ttcatctctt ttcgggtata
     9121 gagatatccg gtgggattgt ttggattgca tatcaggatt cctttggtgc gtggagtgat
     9181 gagttcttca aactgttcga taggaggcag ggcaaatcca tcgtcgatac tcgataccac
     9241 aggtcttacc acagctccgg cacctatggc aaaagccgta taattggcat aagcaggctc
     9301 aggcacaatg atttcatcgc ccggatccag gcaactcata aaggcaaagc taacggcttc
     9361 cgaacctcca gtggtgatga taatgtcgtc gaaagctacg tcgatattaa acgaacggta
     9421 atattcggcc agtttgaggc gtaaactttt gaaaccatcg ctcggactgt attcgaggat
     9481 ttcacgatca atattccgga tggcatccaa tgccactttg ggtgttttga tgtccggctg
     9541 accaatgttc agatggtaca cttttacacc acgagccttt gctttttctg ccagcggtgc
     9601 aagttttcga atgggcgatt cgggcattga ctgtccgcgt tgcgatgtgt tgggcatagt
     9661 ttgtttttga agccctccaa ctcctgcagc aggagttaag aaaggcattt gtgataattt
     9721 atttatgtat ttctttttcc ttttcttgaa attgttgcat tttttgtaag tctcacctgg
     9781 agtttttttt ggaattccct attttatatc caccatcagt ttcaggttaa attgtcttcc
     9841 tgtcaagtag ttgggacttc cgtgctgata tccgtaaata tctgataccc aataaataga
     9901 attctcgttt ttgaaatcaa acaagttgaa aacttccaga tttaaccaga tggaagcaat
     9961 atttttcatc catcgtttat tcattatcct gtccgaacca tttacaatca cgcgtgaagc
    10021 tccgatatcc acccgtttgt agtcgcgcag ccgaaaccgg tcggccatgt agcgcgggct
    10081 gcgtggcgga ccaaccggca atccatctga aaaaatggtt ttcagataga gtttgtattt
    10141 cggattattg ggcagatagt cggaaagaag catggagagg ctgtaacggg cttcattcgg
    10201 acgtgaaatc cagccgggat acaccttgtt tccttcctta tcagtgtaaa aatcgttcag
    10261 cacatcttcc cgtgatcgca tcagtgagaa atttatccac gagtcagttc cgggtaccag
    10321 ttcaccaaat aacttgaaat ctacacctgc cgtatatgct tttgcatcat tctggccgga
    10381 ataacgaatg cggacattgt ccacagaata ggtcgttacc ctgtcggcca gtttcaggta
    10441 agcttcggtg gtaaacttaa acgggcgtcc gagccagcga aaataatggt cacccccaag
    10501 cactaaatgc agcgaacgtt gcgctttcag gtttggattc aggtgtacgg taacattgcc
    10561 cagtgcatcc gaaacggtgt cgcggagttc tttgtagaat ggcgactgat agtacaagcc
    10621 cgttgccagc cggaaattga aatccttgtc ccagtggggc aaaatggaga ctgatgcacg
    10681 cggacttaac agaaattccc ggttatacga ccagtgactg gctctcaatc ctcctgtaag
    10741 tgcataaagt gcatgttctg tgtcccacct gtacgtatcc tgtatgtaag cggatgttcg
    10801 tcgtgaatcg agcttttgtg tggatttcat attgtaaaac agatcaacaa ccttatcgtc
    10861 cgtgtaaggc atcgagtaac cggcactgtc gcgccattcc cactcgttta ttttgtccga
    10921 aataatttca cgctggaggt ttactcccca tttaagtgta tgtcctgatc tgatatattc
    10981 tcctgaatgt gagatgtttg ctactgtggc tttcaggcgg tttcgggcat gctcgtggta
    11041 agtgcctaca cccagttttt caccttcttc agtttcccct gtatcgccca ttttcagttc
    11101 tccgagtaca taattgccgg ttatatcgta agtttcgttt tcgttggtgg aaaatgtgga
    11161 agtggtaatg ctcagtttca ggttttcctt gggcctgaaa agaagggaca gtgctccgaa
    11221 tgctgtttga aaaaggtctt tttcctgtcc ttcgtaatac acatttaact tttcggccat
    11281 gttgaatgta ccgaatgcag tggattgtga atcagggatg aaagtgtaat tatttctcga
    11341 aaagtttccc agaaagttaa tttctacccg gggtgaaatt tgataggtga ggtaagtctg
    11401 ataatcgatg aaatcgggct tgtacgatcc tttcgtatcc agcgttccaa ggaggtattt
    11461 cgaagtcttg tagcgaatgc cgtgcatctg tgtaaaccgg ttgccggccg tacccaagta
    11521 agcggaagct cccagcagac taatcgcacc cgaagcctca aaagaaatgg gttttttata
    11581 tttcacatcc agcaccgacg acattttatc gccgaattcg gcattgaaac cgccggatga
    11641 aaatgccaca tttcccacca tgtccggatt gataaaactc agtccttcct gctgtccggc
    11701 gcgaatgagc agcggacggt aaatctctgt tccgttgaca tagacaatat tctcatcaaa
    11761 atttccaccg cgtacgttgt actgggaact caattcgttg ttggaagtca cgccggcaaa
    11821 ggaaataagc agcgactcaa aacttccgct tgcattgggc atcagtttat atttttcggc
    11881 ttccagtgtc tgcatgtttg atgtgctgcg ccggtttacg gttacatcca cggttttcag
    11941 ttcccgggaa agtgcctgaa gagctactgt aatctgtaca acttgttttc caggaaaaat
    12001 ggtatggcga attgtttcgt aacccaggtg tgaatagaca atttttactg aatcggtgat
    12061 gttcacatac aaatcgtaat atccgttgcg gttggtgctc gtcccgttgt tcatgccttc
    12121 aaaacgtacc cctgcatctt caattccacg gttgttttcg tcaatcacat agccgtaaat
    12181 cctcactttc tgaccttgtg caaacacaga tgcaactgaa agcagaaaaa acaggatgaa
    12241 gattatttta tatctcatag acaggatgct gagtgccgaa tcaacaaata tagtaagtaa
    12301 acggtcgagt gaatggttta ttgaataatt aactcacaaa attagtgata aaatagcttt
    12361 tgacaaaaca tgtgtaaagg aattcggtaa aatgtgaaca gtttgtgatg acattatgtg
    12421 ctattgcaaa attaaacttt atagaaaaca actcagatcg gattttgaaa taatcaacct
    12481 ttcgggataa tttgcctgcc tctttttttc ctttcggagt attattacta actttgaaac
    12541 gaaaaaataa ccaaaaaatg acgggaaaca gtgaaggtat ttttcagcgg acggaattac
    12601 tccttggaaa tgattttctg aggtgtgcgg ctgaaaagcg tgtaatactt tttggagtgg
    12661 gcggagtagg gagctggtgt gccgaaacgc tcattcgttc gggcatcgta catcttacgc
    12721 ttgttgattc cgaccgggtg agcatgacca atgtgaaccg gcagctaatg gctactacca
    12781 aaacagtggg tttggtgaag gtggaggttt taaagcagcg attgcttgaa ataaatccca
    12841 cggccgaaat aatagccctc cagcgcattt acagccccga aacacacgag actttcatgc
    12901 tggagtcgta tgattttatt attgatgcaa tcgacagcct ggcaaacaag gtatatctga
    12961 ttcagcaagc cacaaaaacc aatgcacggt ttttctcctc catgggagca gccctgaagg
    13021 ttgacccgac ccgaatccgg gtggacgaat tctggaaagt gcagggttgt ccgctggcaa
    13081 aggcattgcg aagcaggctg aaaaaaacag gaggcgtagc caagaaattc acctgtgtct
    13141 atagcgacga attgcttgaa aataagggcg atatgccgac cggacaggtt gaaaataatc
    13201 ccgaagcgta tgacaatgaa ggtagtattt gggatagccg caaagcccgt atcaacggta
    13261 ccagctctta tatgccggcc atgtttggaa tgaccataac ctcgctggta gtgaaagcta
    13321 tcatgaacga aaagtaacgc cgcggaatta ttactcgttt tctctgagca tacgtttaat
    13381 gtcgtcgcga tggccgtaaa ccaccagaat atcattttct tctatccggg tattggcagt
    13441 aacaatttca cctgctttga tgatcgttcg gcttacaccc agtttattga tttcagacac
    13501 ttgttttaat tttgtgagaa cgattacgtt gtaaattttg ttgaaattga tgttcattac
    13561 cgttcttccg atcatttttg ccggcactac cagttcaacg atgttgtact cttttgtgat
    13621 ttcaaacaga tttacaaaat ttgcatttga taacttcagc gcccaccgat ctgcagtttc
    13681 tatttcagga cgaacaatct ccgtaattcc catcgcttca agtacatttt catgaagtgt
    13741 tgacacagag cggcttatca gacgtttgac ctgcattttt ttcagcaatg cggttgtcat
    13801 caggttttca ccctcattct cgccgatgca gacaataaat atatctgtat ttgcaatggg
    13861 cagggtgtta atggctgccg agtcggtgct gtccaggcaa actacgtggg ttagcttgtc
    13921 tttcagatca tccactttat ccatattttt atccacgcca atcacttcgt gacccatgtt
    13981 tgtaagtctc tcaccaagtg aagctccaaa atgtccgagg ccgatgatta taaatttcat
    14041 aaaccaatag tttttttaat taattaaaat ttcttctgaa ggataacggt atttagtatt
    14101 aactgatttt ttaattatag ccatcaggaa agtaatcata ccaacccttc ccaaaaacat
    14161 ggttatcaca ataattattt tactggcatc ggtgagcgag ccggtaattc cacggctcag
    14221 acctactgtg ccgtaagctg atacaacttc aaaaacaata ctcaacaggc ttttgtccga
    14281 atcgaatgcc gatacactta tcgacgaaac aaaaatgact gcaaacgaaa gcagaatgat
    14341 tgccgaagat ttgttgatgg ttacctgaga aatttcgcgt ccgaagattt caagactttt
    14401 tttgcccctg gcaatgctga tcacattcat gcaggcaact gctatggtgc tggttttaat
    14461 accgcctccg gtcgaagccg gcgaagctcc tatccacatc agcagcatga aaatcagcgt
    14521 gacaggaagt gtcatggcag ctgtatcaac tgaattgaag cctgcagtac gtgtggtaac
    14581 cgatccgaaa aatgaaacga ccaactttcc aaagaaactc cgatgctcgg taagtgtatt
    14641 gttatactcc aacaaaaaaa tgaaaacggt gccggatata agcagaatgg ctgttgtggt
    14701 aattattact tttgaattca ggttgagcac ccagggtaag taagtctttt ctctctttct
    14761 gacaagtcgt ttgaaactat tccctatttt ataaataaaa tatttccata cgttaaatat
    14821 tatgggaaat ccgataccgc cggtgataat gagcagggct atgaccaact gaaaaaagta
    14881 attgtaacgg aatgtaattt caaataagtt gtttttcaac ggtgagaaac cggcattgca
    14941 aaacgccgaa accgagtgaa aaaccgaaaa ataaattcgt tgacctactc ccatcactac
    15001 gtcgttgctg atgcttgagt aaagaaaaac agctcctgat gcctcgatac tgaatgttac
    15061 cagaattatc tttttcaaca cattgaaaac gtcgtccagt ttatccacat tactcatttg
    15121 gcccatatac atctggttct cataggttgc accgcttctg aagaaataac tgaaataaga
    15181 tgtaaaagtc ataatcccga gtcctccgat ctggatcagt gcgatgataa tagtttgccc
    15241 tatcagtgta aatacacttc cggtatcaac caccgtaagt ccggtgacac aaacggcact
    15301 tgtagctgta aaaagggcat cgacataaga aatatcgccc cgggtggcat tcggaagcat
    15361 aagtagcagg cttcccatga ggataatgat aaaaaaactg gctacaaaaa gttgtgccgg
    15421 attaataagc ttatacctga aacggaagtt aatgttgtaa acttcacgta aaaataccag
    15481 aaacaaagca atgtaagtcc acgagttgtg catcgaatga aatccgaaag taaaaagacg
    15541 ggctaaattg tgaatgagca caaagatcag gaaaatggac agaatcatat caatcctgac
    15601 aattttatgt tgtaagttgt ttcgtttgat atatattctg gcaagcagtg aggtgaggca
    15661 taaagcaatg gccagatggt atataaacag aaaaaactgg tcggttctgt attcaagacg
    15721 aaagccgaga tcaacaaccg aaagtataaa cgcaattaca gctaatgtaa atgatgcttt
    15781 gtttatacct aataaattac ggtatgcatt tggtttttct gccattgaac aattaatttc
    15841 aaaaaaaact ttgcaaatat agatattttt ttatctttca ttgttcgcgg taaccggaaa
    15901 aatgttattt atttgattcc gttcttctga tccggcattt tcattttttt tctgtaaatt
    15961 ctttaaaaat catgttcttg acacattttt cacccctttt acgtttttca cttttttgat
    16021 gaggtcgtcc agtgatttgt tgtcgttcac catcacggta agatttccct gaaacaatcc
    16081 ggcgttcgtg tcgatggaga tggagcgaag cattacctct ttttctttgg aaataatgga
    16141 tgtaatgttg gttacaatcc cgatatcatc gtgccccacc acgcggagtg taatagggta
    16201 ttgcgaccct tccgatttgc ccgaccatct tgctttgata atccggtatc cgaatcgtga
    16261 tatcatctgt ggagcattcg gacaattggt gcggtgaatt tttattccac ccatcaccga
    16321 tacaaaaccg aaaatctcat ctccgtagat tggattacag cattttgcca gcttgtagtc
    16381 cacgtttcgc aggttgttgt caattaccag tacatcttct ttcgaagccg tttcgggtat
    16441 ggcggcaact tctttaacga aattttcagc agtacggttt tcggtggcag tggtattgtc
    16501 acgtgtttcc agttccaggt acaattcccg aacttcgtga atgtccactt tttcatgtgc
    16561 tatagcctga taaagatcgc tcaccgtttt atagcccatt tttttcgaaa tgcgtgagaa
    16621 acgcccgtct tccagttcaa ttttccagtt tttgaaccgg cgtatcagcg tttcgcggcc
    16681 tatttcggca tccttgaatt ccacttcttt caacgattgc ctgattttat ttttagcctt
    16741 agaagttgta acgatgttga gccaggcagg attgggcttt tgggtgggcg aggtgagtac
    16801 ttctacctga tcgccgttgt tcagtacata ttttatctgc acatgttttc cgttcacctt
    16861 gcccccaaca cactgagtgc ccagattggt gtggattgaa aatgcaaaat ccagcagtgt
    16921 ggcgtctttg gggagtttgt gcaggtcgcc attcggagtg aaaacaaaaa cttctttgtc
    16981 gtacagattc aacttgaatt catccataaa gtccacggca ttcagatcgg gattttcaag
    17041 tatctcacgg atgtttttga gccattcgtc catgccgctt tcgcttttga tgcccttgta
    17101 cttccagtgt gcagccagcc ccatttcagc tacttcattc atcctggctg tacgaatctg
    17161 cacttccacc catttatttt gcggaccgag taccgtggtg tggaggcttt catatccgtt
    17221 tgatttcggg atggaaagcc agtcgcgcag tcgcttcgga ttgggttgat acatgtctgc
    17281 cacgatggaa taagcctgcc aacaggcagc tttttccttt tcgatgggag tgtcgaaaat
    17341 aatgcgaatg gcaaacaggt cgtagatatt ctcaaacgtg gtgttctgtt tttgcatttt
    17401 cgaatagatg gaatgaatgg atttcgtccg accttttatt tcaaaaggaa atccgatttc
    17461 ctccatttcc ttcttcaatg gttcgatgaa tgcattcata taggcatccc gcgaccgttt
    17521 agtctcgttg agttttcggg caatcgcttt gtaaatctcg cggtcggtga atttaagcga
    17581 cagatcttcc agttcggttt tcactttgta aagccccata cgatgtgcca gcggagcata
    17641 taggaatgag ctttcgcggg ctatttgctg ctgctcgtca gtgggatatg actccaggtt
    17701 acgcatggtc tgtaagcgta cggccacaat aatgaggata acgcgcacat cttcggccat
    17761 ggtaagcagc aactttcgga aattctccga ttccagtgct acgttttttt gatacagctc
    17821 ttttacccgc tgaagaccga tcatgatagg tgaaatggaa gtgctaaaca atttatctac
    17881 atctttaacg tcaatatgtt gtttttcaac tggttccatt agcaggaaac tgatcacagc
    17941 tgcctttccc agtccaattt cgttcagcag aatatccatt atctccaggt tccttttcag
    18001 aaaagcctcc gggttttcca caccgtattt tttttcggat aaaaaatgtt tcagaatacg
    18061 gctcagttcc ctgcgctcgc tgtgtgtaag gagcgtggag tgtgaagtat aaagatttcg
    18121 gtacaaccgt atgttttctg cattcatttt gatgaggttt acttttattt ctgcaaatat
    18181 actaattcgt acgaataact cgcagcggaa tttttctgaa tttcttcaaa tttcgttaat
    18241 aaaaaaagaa cggattgatt ttggtaagca atcatttctt tcgtaatttt gttgaaatta
    18301 tggaatcagt tgccttttca ttaaattatt aacgctaaaa tgcctgaaaa tcaattcatt
    18361 agtaattcgt tagaaaataa cctgctgatc agtattcgtt ctgattcgat actttattcg
    18421 gtgactgatg agaacgagca ggttcttttg cgtcaggaaa tcaatatgct gcccggtaaa
    18481 atggccgacg ctactgttta cgggcatttt ttcaaccagc cggagctcaa tattttgagt
    18541 gaaaatgtgt tgattttaat tgagaacagt cattttcagc ttattcccga cgagcttttc
    18601 agggaggaaa atatgcgtga gctttttgag ctggaattcg gtaaaatgga gaacagcaag
    18661 attaaataca tacttctgcc taaatgggga gctcatctcg tattcggagc gcctgccgcg
    18721 atgattgatt tttttgaagc gaaatacccg gatgccgtca tcgaacatca tatttccggt
    18781 ttgctgaaaa agaaaatcaa caagtcggat gaggcggttt ttgcctattt gcgtaaagac
    18841 acggtggatg ttcttgtggt gaaagacaac ctgcttcagt tggtcaattc gtttgatgtg
    18901 aaaaccacag aggattgctg ctactttgtg ctgaatgtgt atgagcagct tcaactgaat
    18961 accgaaagta tgactttagt tttgatgtcg gaaaattcag tgaatgaaga atttatagat
    19021 ttgttgaaaa aatatatagt aactgtaaaa gtgaaataat atgcggataa taggcggaaa
    19081 atacaaagca aaaaggataa gtgttcctca taacatcacg gcacgtccca caaccgattt
    19141 tgccaaagag ggattattca acctgctgaa caaccggata gattttgaag acatcgatgt
    19201 gcttgatttg tttgcaggca ccggaagcat cggttttga
//
LOCUS       DAORKP010000025        19117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_146_length_19117_cov_8.685972, whole genome shotgun sequence.
ACCESSION   DAORKP010000025 DAORKP010000000
VERSION     DAORKP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19117
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_19117_cov_8.685972"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLN75_02650"
     CDS             <1..850
                     /locus_tag="PLN75_02650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRG03048.1"
                     /translation="GRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     EEMKWFEYWKQFRLRWHKALGLGDHKYRFHDHDKLAHYANAATDIEFEMPFGFKEVEG
                     IHSRTDFDLSQHEKYSGKNIKYFDPELNESYVPYVIETSIGVDRTFLSIMAAAYTEEE
                     LEGGDSRVVLKFPPVLAPVKAAILPLVKKDGLPEKAREVMNELKFDFKCVYDEKDSIG
                     KRYRRQDAVGTPFCITVDHQTLEDNTVTIRHRDSMEQDRVQIDELQKIIDAQVNMKNL
                     FRKITQ"
     gene            complement(1041..1520)
                     /locus_tag="PLN75_02655"
     CDS             complement(1041..1520)
                     /locus_tag="PLN75_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRG03049.1"
                     /translation="MQRNDNIEIERKFLVRGEFMSHVSQEHRIRQGYIVASAGKTVRV
                     RTRGDKGFLTIKGPSGKNGFSRFEWEKEISFTDAEQLLELCEKNIIDKTRYEVEFDGK
                     TFEVDVFHGVNEGLVLAELELQTEDEPYSAPEWLGREVTGDMRYYNSYLSKYPFSEW"
     gene            complement(1533..4415)
                     /locus_tag="PLN75_02660"
     CDS             complement(1533..4415)
                     /locus_tag="PLN75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRG03050.1"
                     /translation="MFLQRIAKAFYTAYEADIRKFTFVFPNRRAGLFFQKYLTELIDK
                     PVFSPEIITINDCFFNASTRRVADRTAELFRLYRIYRQVSKSEETFDAFVFWGEMLLT
                     DFNDVDKYRADPRQIFTNVKELKEIDQLSEYISDDQKAAIEQFWKHFLPKIESKTKKE
                     FIAIWCILLPLYEEFCSELRSENLATEGMIFREVVDKLRQNDDAEYFRDKKFVFVGFN
                     ALNKSEQMLFEELRKRHQADFYWDYESDELRDEDNQASGFYRENIRNFPSALTIDPEV
                     LPLSGKSFELIAVPSSVGQTKEVFNLLNLLFPEDSPENDLIKTAVVLPDESLLVPLLH
                     SLPETVEKVNVTMGFPLKATPVSGLLENLFELQRRMNARGQFYHITVSNILNHQYINM
                     LCGEQIRIIHQEMVANNSFYVDESLFANDELLKTIFTSKKDPAEFMTYLLDVLKKLNV
                     AWQRFAGESEKYSLECDFLYQYYTALNRLNDVMKSIGRFEMGIDTLVRLIRQLVGGIS
                     IPFEGEPLNGLQVMGMLETRGLDFENLIICSFNEGVFPKKSTSSSFIPATLRRAFGLP
                     VSEYHDAISAYNFYRLIQRTRRIFFLYDSRSEGMQTGEVSRYIHQLRYHYNVDIKQVN
                     VTYEINSADENIVQVSKTPEIMEKLALYLTSGENERALSASSLNTYIQCPLQFFLKYV
                     EQMEELEEVQESVESGMFGTLLHEVMEKLYEPFVGKMVQKQELTELSKNNLLIDKTIT
                     RAFATRFFKKKNEAADLVELEGNYLLTARVIHQFVEKILLTDASRAPFRYIQSEKNIL
                     IRYPFENGTLHVNLKGYIDRVDEKDGMTRILDYKTGGDELKFNSVGELFERNNKKRPK
                     AVFQTFLYCLLYNQKAGTENLVPEVLKVRDLFKSNFTTQLYDKVKDQYVEDFNVYRDE
                     FSEKLTELIREIFDAEVPFEQCTELELCRYCTFRAICRREVKTLNY"
     gene            4659..5357
                     /locus_tag="PLN75_02665"
     CDS             4659..5357
                     /locus_tag="PLN75_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRG03051.1"
                     /translation="MLGTIVNTIAVIAGGSLGIFLKKGMPERIRTIYFQAVGLFTLAI
                     GISMVWKMDHILTVVTSVVVGAMLGEWMNLEKGAEQLSEWTKKKLRIGSERFSEGLTT
                     SFLLFCIGALTIMGAIEEGTTGSARLLLTKSILDGFSSILLASAFGVGVVFSAIPLFL
                     FQGGITLLARFFGEFLSAETIQALTSVGGILLLGLGINILEIKKLRILNMLPALVVVV
                     LLMWIMKYLPMLNF"
     gene            5472..5912
                     /locus_tag="PLN75_02670"
     CDS             5472..5912
                     /locus_tag="PLN75_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03052.1"
                     /translation="MNLHLAIIWFLVGFVLVNGLKALHIFGSGKRQEKKSVNKNVNPA
                     QFTQYAYRKSLPYTPVYYLTVWTLCSTFYFLQHTPANIYKDALLTGIFWWLLTLLLEM
                     LLWTVVRHRFQLTPKEMYMHSQPWISLAYYAVLISPLVLSMILA"
     gene            6156..7688
                     /locus_tag="PLN75_02675"
     CDS             6156..7688
                     /locus_tag="PLN75_02675"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRG03053.1"
                     /translation="MNKRKVFEQRLKSLQNMEEIAVQKQHLKAKLTARERLNILFDEG
                     SFFELNTFVEPAKSIKKTQNAYGDGVVVGRGLVQGRSVFAYAQDFTVMGGSLGYAHGM
                     KIAKVQEMALKFGSPCVGLIDSGGARIQEGINSLSAYARIFKNNVQSSGIIPQISVIL
                     GPAAGGAVYSPAMTDFVIMTNKTSYMFVTGPDVVKEVLNQDITMDELGGGNVHAEKSG
                     VAHFVFEDEEHAIIQVRKLLSYLPLNNFDALPVSTDVDFNEDRQEYLNKIVPDDPNRP
                     YDMKEIINTLVDNNSFLEIHEKFAQNIVVGFARLEGKTIGIVANQPKAMAGTLDINAS
                     IKGARFVRFCDAFNIPLLVLEDVPGFLPGVDQEHTGIIRNGSKLLYAFCEATVPRITI
                     ITRKAYGGAFVVMNSLGIGGDFNFAWPTAEIAVMGPAGAIKIVNRAELTAATDREKLE
                     KELIEKYKEEIANPFKAEELGMIDDVIMPSETRRILVTAFDILSNKQYSQPERKHGNI
                     PL"
     gene            7888..9381
                     /locus_tag="PLN75_02680"
     CDS             7888..9381
                     /locus_tag="PLN75_02680"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRG03054.1"
                     /translation="MKKILIANRNEIAIRIIRSAKQLGLTTCVIHCNREPDALYLEYA
                     DEIIYVKEDLTGKPIFLNPEAIVKLALEHQIDMIHPGYGFLSENPDFAALCEANGIIF
                     VGPSPELIRNMGIKTIAKEMAARAEMPLVPGSPGAITDALLAKEFADKIGYPVILKAS
                     AGGGGRGMRIVEKPETMERHFKSAHEEALAAFGNGDMFIEKYLVNPKHLEFQILGDKH
                     GNVIHLGERECSLQRKHQKIVEEAPSASISDENRVKMGELAVKFAKSIGYYSAGTIEF
                     ILDEDGEYYFMEMNTRIQVEHPVTEMITGTDLIAWQIRIALGETLTLKQEDIQFNGWA
                     IECRVNTEDPQNRFSPQTGFIEKIHFPHGDHIRIETGVKDFSVVTPYFDSMIAKIIVH
                     GENREDCIRKTLTALNDFSISGLKTTVPFCKTVLRSKEFVEATYTTRWVDTVFAPEML
                     ESEDEAMMAALAATISYAKEYLQYSSESPMFKSESLNVWVLNKRINK"
     gene            9555..10103
                     /locus_tag="PLN75_02685"
     CDS             9555..10103
                     /locus_tag="PLN75_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRG03055.1"
                     /translation="MATTYVYKRDATRLFIAMRDNGSRYKIYLPKESNPIINGKEKDI
                     FFIEHKNNFSTFSMNDTLYRCEITQRDQNRIVVKVNGVEYKFSLESIFSYLRRNMLNK
                     DAGAASDNNIRSQMPGKIVDIFMSEGDLVNEGEPILSLEAMKMQNEINASCNGVIRKI
                     HVVPGQSVMQDELLVEIASIDV"
     gene            complement(10189..11451)
                     /locus_tag="PLN75_02690"
     CDS             complement(10189..11451)
                     /locus_tag="PLN75_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRG03056.1"
                     /translation="MKLKFLLCIGITAFFMSSCATTSHNKLTLEEKNAGWQLLFDGKT
                     LNGWRDYNGEKLTAPWFAANGTIQAKGGGGDAHGYIVTKKNYENFDLVWDWKIAEGGN
                     SGMLYHVVENPAFKVPYVTGPEYQLIDDIGFPEKLEDWQKTAADYAMHLADTKKSLKP
                     AGEWNTSRILFDNGHVEHWLNGEKVVEFEAWTDDWFRRKHSGKWENAPEYGLACTGVI
                     CLQDHGSAAWFRNMKIKELPRKSKTVSLFNGKDLTGWEAYGTEKWYVNDGLLVCESGP
                     EKKYGYLATREYYNDFDLTLEFKQEADGNSGVFFRSFIEPDVKVNGWQVEVAPRGNDT
                     GGIYESYGRGWLVQIPEEKESILKEKDWNTMRIRVAGDNVKTWLNGVEMVNLTDEKIG
                     KAQGRIALQIHDGGGIKVLWRNIHLKTL"
     gene            complement(11579..12859)
                     /locus_tag="PLN75_02695"
     CDS             complement(11579..12859)
                     /locus_tag="PLN75_02695"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRG03057.1"
                     /translation="MDYQETIQYLYDRLPVFHQIGAAAYKPGLENSIRMMSELGNPQN
                     SYKTIHVAGTNGKGSVSHFLAAILQSAGYQTGLYTSPHLVDFGERIRVNGAKVNREFV
                     VKFIEKYKPLFDRIEPSFFEATMAMAFDYFASENVDVAVIEVGLGGRLDSTNIIHPEL
                     SVITNISFDHTQFLGDSLDKIAFEKAGIIKKETPVVIGESLAETKPVFIQKAESENAP
                     VFFAEELQPVRFLRYENGKMIVKTADFEMLEIGLNGIYQLKNVATVLEVVKQLQKTGF
                     NISEQHIRDGLSNVVSLTGLLGRWQTVSENPKIILDTAHNKGGFEYITEQLQQTKYNK
                     LHFVFGMVNDKDISGVLSMLPKDTEYYFTAAETKRALSPEELKNQAKTFGLHGNTYNT
                     VYEAVKTAMMRAGADDLIYIGGSNFVVGEALELF"
     gene            13044..14375
                     /gene="tilS"
                     /locus_tag="PLN75_02700"
     CDS             13044..14375
                     /gene="tilS"
                     /locus_tag="PLN75_02700"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRG03058.1"
                     /translation="METLKKVQEFIQTNELLQPSERILVGVSGGVDSVVLLNILLKSG
                     YECIVAHCNFHLRGSESDRDEKFVEKLAKEYGVMFKKTDFNTTGYARKNKISIEMAAR
                     ELRYEWFNRVAKEIHSGSIAVAHHADDSIETFFLNLVRGTGLKGLTGIDAKNGNIVRP
                     LLTCTRQEIEKYAKINNLQYVFDSTNASNDYSRNKIRNQVIPLLSDINPSVSQTFMEN
                     IIRLRGIRKIYEAKIQNIKEKVLSEKEENIYIDIEELKKQADIQTVLYEVLNEYNFNS
                     KTIREIVSGLEHNSGLQYASPTHRLLKDRNALIIQRMEPRNETEYLVSKNIREITVPV
                     HLHFRWIKNTPDFQISRTADTVHIDADLLEYPLTIRKWRKGDTFQPFGMTQSKKLSDF
                     FINSKLSLYQKEEIRLLLSAEKIVWVIGMRIDNRFRITDETQNILEISYEK"
     gene            14579..16462
                     /gene="rho"
                     /locus_tag="PLN75_02705"
     CDS             14579..16462
                     /gene="rho"
                     /locus_tag="PLN75_02705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRG03059.1"
                     /translation="MTNYNILQLNAKELSELKEIANKMALKVPNSITKDKLVYEILDQ
                     QAIMNAQEKSSVSEENEGNKRKRMRIIPKKAEDQKVFSTENVIKNQPAAEKTEKVVKP
                     EAEKTEKSDKPKQQPPKTETPKADTTPAAKQETKKKTSPKAKKTPAKKEAPTAVKVEE
                     TPVDTKAEAVIAEEQKETKIAPPVRKILQPVKEEIKTVIDDQLLQEIINEEETNETDI
                     ATEKEEQPQGTHKHQRENQGNGNRVKFNPRPEKQFDFEEVLEGTGTLEILTDGYGFLR
                     SPDYNYLSSPDDIYVSQSQIKLFGLKTGDTVKGSIRPPKEGEKYFPLIRVSKINGRDP
                     AYVRDRVPFEHLVPLFPEDKFTLNSGKNDTLSTRIVDLFCPIGKGQRGLIVAQPKTGK
                     TVLLKEIANAIAANHPEVYMIVLLIDERPEEVTDMARSVRAEVISSTFDEPAERHVKV
                     ASMVHEKAKRMVECGQDVVILLDSITRLARAYNTVSPASGKVLSGGVDANALHKPKRF
                     FGAARNIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKLSNKRIFPSV
                     DIMASSTRRDDLLLDKDTLNRMWILRRHLADMNSMEAMEFLKERLERTENNEEFLISM
                     NRE"
     gene            complement(16758..17180)
                     /locus_tag="PLN75_02710"
     CDS             complement(16758..17180)
                     /locus_tag="PLN75_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03060.1"
                     /translation="MMRFQQVRLFRLNFGNFRRGAALTIPLVGIFVGKGMEDDLNLLR
                     HEFGHILQFRKWGFWFFWRHIAKTSLDSAHASRKEHRKHQHTWTEWSANRLAYYYFNS
                     PDDWDFRRFPIQPTIEDSYSKPTFAQSNDDFMKHWMEA"
     gene            17265..17921
                     /locus_tag="PLN75_02715"
     CDS             17265..17921
                     /locus_tag="PLN75_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRG03061.1"
                     /translation="MDATKFYTKREEKANWITHLLGIMIAIAGTYLLTAKAIVHQNIW
                     ALLAFLIFGFGMFVCMLASTLYHYVQTPKTKSLLRHFDHGSIYVLIACSYSPFTLILL
                     RNEGIWGWGLFVLVWIIALTGIGFNFGTLKANSHLKTASYVLMGMIILIAIKPSIEVA
                     ASLGCMNVLLWLLIGGIFYIAGAVIYALAKHEFIHAVFHVFVLLGMMSHIYAAYLIPL
                     "
     gene            complement(17918..18388)
                     /locus_tag="PLN75_02720"
     CDS             complement(17918..18388)
                     /locus_tag="PLN75_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRG03062.1"
                     /translation="MTTNPQTCDDYVAGLSDDRKIVIEKLRKVLKENLPIGYEEEISY
                     GMIGYVIPFSIYPAGYAVKPKVPLPLMNLASQKNHVALYHMGVYSVPGIAEWFEAEYP
                     KHCKTKPDMGKGCIRFKKMDDIPYELIGELARKVDVQTWIDTYENVIRRPKKQD"
     gene            complement(18568..>19117)
                     /locus_tag="PLN75_02725"
     CDS             complement(18568..>19117)
                     /locus_tag="PLN75_02725"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154925.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRG03063.1"
                     /translation="IDYRKDVDGFHPVNVGRMSIGLPCYVSATPYGIMELLKRYEIPT
                     SGKHCVVIGRSNIVGKPIASLMMQKSYPGDATVTVCHSRTQNLKEITLQADIIIAALG
                     KPEFLRGDMVKEGAVVIDVGTTRVPSTETKSGFRLKGDVKFDEVAPKCSYITPVPGGV
                     GPMTIVSLLKNTLLAAKGEIYS"
BASE COUNT         5583 a         4301 c         3999 g         5234 t
ORIGIN      
        1 aggccgcatg aaaatcccgt ttggcattgc tcagataggt aaagcattcc gcaatgaaat
       61 cgttgcccgt cagtttatat tccgtatgcg cgaattcgaa cagatggaaa tgcagttttt
      121 cgttcgcccg ggcgaggaaa tgaaatggtt cgaatactgg aaacagttcc gtctgcgctg
      181 gcacaaagcc ctcggactgg gcgaccataa ataccgtttc cacgatcacg acaaactggc
      241 gcattacgcc aatgctgcta ccgatattga atttgaaatg ccgtttggat tcaaggaagt
      301 ggaaggcatt cattcacgta ccgatttcga cttgtcgcaa cacgaaaaat actccggcaa
      361 aaacatcaaa tacttcgatc cggagctgaa tgaatcatac gtgccctacg ttatcgaaac
      421 ctctatcgga gtggatagga ctttcctttc catcatggca gctgcataca ccgaagaaga
      481 gctcgaaggc ggcgacagcc gtgtggtact caaatttcca ccggtgctgg ctcccgtaaa
      541 agctgccata ttgccgctgg taaaaaaaga cggactgccc gagaaagccc gagaagtaat
      601 gaacgaactg aaatttgact tcaagtgtgt gtacgatgaa aaagactcca tcggcaaacg
      661 ttaccgccgt caggatgctg taggtactcc attctgcatc acagtggacc accagacgct
      721 ggaagacaac accgtaacca tccgccaccg cgacagcatg gaacaagacc gcgttcagat
      781 tgacgaactt cagaaaatca ttgatgcaca ggtaaatatg aaaaacctgt tcagaaaaat
      841 aactcagtaa ttttcggata tgatggtgga gtcaggatgc ctgaataagt tgaaaaaacg
      901 gctctgaaac tattcataca gacagaatac aggaaaaagt ttacctgaaa atcatcgggt
      961 aaactttttt ttttcacaga cggtaaagtg atgaatatgt gtgagaagtt tctaaaaaat
     1021 acaactatct caggccggag ctaccattcg ctgaaagggt atttcgacag gtaggagttg
     1081 taataccgca tatcgcccgt cacttcccgg ccgagccatt cgggtgcact gtagggctca
     1141 tcttcggttt gcagttcaag ttctgccagc accagccctt cattgactcc gtgaaaaacg
     1201 tccacttcga aggttttacc gtcaaattcc acttcgtaac gtgttttgtc aatgatgttc
     1261 ttttcgcaaa gctccagcaa ctgctctgcg tcagtgaatg agatttcctt ttcccactcg
     1321 aaccggctga aaccgttttt acccgaagga cctttgatgg taagaaagcc tttgtcgccc
     1381 cgggtgcgca cacgaacggt ttttccggcc gatgccacaa tgtatccctg cctgatgcgg
     1441 tgctcctgcg aaacatgtga cataaattca cccctgacca gaaattttcg ttcaatttcg
     1501 atgttgtcgt ttctttgcat atattttttc ttttagtaat tcagcgtctt tacttcccgg
     1561 cggcatattg ccctgaaagt gcagtagcga cacaattcca actcggtaca ttgttcaaac
     1621 ggcacttcgg catcgaaaat ttcacggatc aattcagtca gtttttctga aaattcatcc
     1681 ctgtacacgt tgaaatcctc tacgtattgg tctttaactt tgtcatacaa ctgtgtggtg
     1741 aaattgcttt tgaacaaatc gcgcactttc agcacttcgg gcaccaggtt ttctgtacct
     1801 gccttttgat tgtaaagcag gcagtacagg aaggtttgaa ataccgcctt tggccttttt
     1861 ttgttgtttc gttcgaacaa ttcacctacg gagttgaatt tcagttcgtc gccgccggtt
     1921 ttgtaatcca gaatgcgggt catgccgtcc ttttcgtcca cccggtcgat gtatcctttc
     1981 agattaacgt gcagtgtacc gttttcaaac ggataacgaa tcaatatatt tttttctgac
     2041 tgaatgtacc tgaaaggtgc tctcgaggca tctgtcagca gtattttctc aacaaactga
     2101 tgaatcaccc gggctgtgag caggtaattt ccttcgagct ccacaaggtc tgcagcttca
     2161 tttttctttt tgaaaaacct ggttgcaaat gcccgtgtaa tggttttatc tatcagcagg
     2221 ttgtttttgg ataattcagt cagttcctgt ttctgaacca ttttacctac aaagggttcg
     2281 tagagtttct ccatcacctc gtgcagcaaa gtgccgaaca tacccgattc cacgctttcc
     2341 tgcacttctt ccagctcttc catttgctcc acatacttca gaaaaaactg cagcggacac
     2401 tggatgtaag tattgagcga acttgcagaa agagcccttt cgttttcgcc gctggtcagg
     2461 tacaaagcga gtttttccat tatttcggga gttttactta cctgaacgat attctcgtcg
     2521 gccgaattta tttcgtacgt aacgttaacc tgcttaatat caacattgta atgataccga
     2581 agctgatgaa tataacggct cacctcaccg gtctgcatcc cttccgaacg gctgtcgtag
     2641 aggaaaaaaa tccggcgtgt acgctgaata agccggtaaa aattgtaagc cgaaatggca
     2701 tcgtgatatt cggatacagg cagaccaaag gctcgacgaa gggtggccgg aataaaactg
     2761 ctggatgtgc tctttttcgg gaatacgcct tcgttgaacg aacatataat caggttttca
     2821 aaatccagac cacgcgtttc gagcattccc attacctgaa gtccgttgag aggctcgccc
     2881 tcaaacggga tggaaatacc gccaaccagc tgacggataa gccttacaag cgtatctatg
     2941 cccatttcga aacggccgat gctcttcatc acatcattca gccggttcag ggctgtgtaa
     3001 tattgataca gaaaatcgca ttcgaggctg tatttttcgc tttctccggc aaagcgctgc
     3061 caggcaacat tcagtttttt cagtacatcg agcagatacg tcataaattc tgccgggtct
     3121 tttttcgagg tgaaaatggt tttcaacaac tcatcgttgg caaagagcga ttcatccacg
     3181 taaaaggaat tgttggcgac catttcctga tgaattatcc gaatctgctc accacacaac
     3241 atattgatgt attgatgatt gagaatattg gataccgtaa tgtgataaaa ctgaccccgt
     3301 gcattcatgc gccgctggag ttcaaagaga ttctcaagca atcccgaaac gggtgtggct
     3361 ttgagcggaa atcccatggt gacattcact ttctccacag tttcaggcaa cgaatgcagc
     3421 agcggcacca gcaaactttc atcgggcaaa accactgcag ttttaatcag gtcgttttcg
     3481 ggcgaatctt ccggaaaaag caggttgagc aggttgaata cttccttggt ctgccctacc
     3541 gacgagggaa cggcaatcaa ttcaaacgac ttgccgctga gcggcaatac ttcggggtca
     3601 atggtaagcg ccgaaggaaa gttccggatg ttttcgcggt agaatccgga ggcctgatta
     3661 tcttcgtcgc gcagttcatc ggactcgtag tcccagtaga aatcggcctg atgacgcttc
     3721 cggagctctt cgaaaagcat ttgttcactt ttgttcaacg cattgaaacc gacaaaaacg
     3781 aatttcttat ccctgaaata ctccgcatcg tcgttctgcc ggagtttatc caccacttcc
     3841 cgaaaaatca tcccttcggt ggccaggttt tcgctccgca gctcgctgca gaactcctca
     3901 tacagcggga gcagaatgca ccagatggca ataaattctt tcttggtttt gctttcaatt
     3961 ttcggtagaa aatgcttcca gaattgttcg atggcagctt tctggtcatc ggaaatgtat
     4021 tccgacaact gatcaatttc tttcagttcc ttcacgtttg tgaaaatctg gcgcggatcg
     4081 gctctgtact tatccacatc gttgaaatcg gtcagcagca tttcgcccca aaaaacgaat
     4141 gcatcaaatg tttcctcact tttgctcacc tgcctgtata tccggtaaag cctgaagagc
     4201 tcggcagtgc ggtcggctac acggcgggtg gatgcattga aaaaacaatc gttaatggta
     4261 attatttcgg gcgagaaaac cggcttatca atcagttctg taaggtattt ctgaaaaaaa
     4321 aggccagccc gacggttggg aaaaacgaac gtgaacttcc ggatgtcggc ttcatatgca
     4381 gtataaaatg cctttgcaat gcgttgtaga aacatgttac tccactgcct cccgaaggag
     4441 gacttttttt aaagtataat gaacagaact tgtataaaaa atttaaatgg ctaatttact
     4501 tcattttatt gaaacataaa actcatttcg gagaaaattt cttacttttg tgaaacataa
     4561 aaaaaattac aaccagggac aagtcgcggc ttgtatctgt taaaaaaaaa tccgaaaagg
     4621 tgagtaaggg aatcaccacg gatcattttc agggctttat gttaggaaca atagtaaata
     4681 cgatagctgt aattgcaggc ggctcgctcg gcatttttct gaaaaaaggg atgccagagc
     4741 gtatccgcac catctatttt caggcggtag gactttttac ccttgctatc ggcatcagca
     4801 tggtttggaa aatggatcat atcctcacgg tggttacgag tgttgttgta ggtgccatgc
     4861 tgggcgagtg gatgaatctg gagaaaggag ccgagcaact gagtgaatgg acaaaaaaga
     4921 aactccgaat tggcagcgaa cgtttttcgg aagggcttac cacctctttt ctgctctttt
     4981 gcatcggtgc actcaccatc atgggcgcta tcgaagaagg cacaacaggc tcagcccggc
     5041 tgctgcttac caaatccatt ctcgatggat tttcatccat cctgctggct tctgcatttg
     5101 gagttggagt agtattttca gccataccac tgtttctttt tcagggcggg ataacactgc
     5161 ttgcccgatt tttcggagaa tttctttcgg ccgaaaccat tcaggcgctc acttcggttg
     5221 gcggcattct gctgctcgga cttggaatta atatcctaga aataaaaaag ctgcgcatac
     5281 ttaacatgct tcctgcactg gttgttgtgg tcttactgat gtggataatg aaatatttac
     5341 ctatgttaaa tttctgaaaa tctgatatct gtgtttttaa aaaaaagttg ccaaacatcc
     5401 tgcatttttt ttaaatttac tccgatagaa aaactccttc gtgagtggat tttgaggcaa
     5461 atgacttatt tatgaactta catttagcaa taatctggtt cctggttggt ttcgttttgg
     5521 tcaacgggct taaagcactt cacattttcg gcagcggaaa aagacaggaa aagaaatcag
     5581 tcaacaagaa tgtcaatccg gcacaattca ctcaatatgc ctaccggaaa agtctgcctt
     5641 acactcccgt ttattacctc acagtgtgga ctctttgcag cacgttttac tttttgcagc
     5701 acaccccggc aaatatttac aaggatgcac tcctgaccgg aattttctgg tggctgctca
     5761 ccctgttgct cgaaatgctc ttatggacgg ttgtcagaca caggtttcaa ctcaccccga
     5821 aggaaatgta tatgcattca caaccgtgga tatcactcgc ttattatgct gtactgataa
     5881 gtccgcttgt actttcaatg atacttgcct gattggtcct tttctttttt gaatctcaat
     5941 ctcatccgtt ttttcgttca aagtactcag aggacaaaaa aaatgtaaaa aaatcatttt
     6001 tctaatcctg aaaaagagta ttttctcttt atccacgaac agaaatcagg taaattttct
     6061 gtcctcgatt ttttttagcc ggtatatttt tcagcatgtg gaaacttttt catgtcctaa
     6121 atgttaaata actattgtaa taattcaacg tttttatgaa taaacgaaaa gtttttgagc
     6181 aaagactgaa atctttgcaa aacatggaag aaattgctgt tcaaaaacag catctgaaag
     6241 caaaacttac agcccgcgaa cgacttaata tacttttcga cgagggaagt ttttttgaac
     6301 tgaacacctt tgtggagccg gcaaaatcca ttaaaaaaac tcaaaacgct tacggcgacg
     6361 gagttgtggt ggggcgggga ttggttcagg ggcgcagcgt ctttgcttac gcacaggact
     6421 ttaccgtgat gggtggctcg cttggatatg ctcacggaat gaaaatagcc aaggtacagg
     6481 aaatggcgct caaattcgga tctccctgcg taggattgat tgacagtggt ggcgcacgta
     6541 ttcaggaagg cattaacagt ctttcggcat atgcgcgtat tttcaaaaac aatgtacaat
     6601 catccggaat aattccacaa ataagcgtga tactcggtcc ggctgcgggc ggtgcagtct
     6661 attcacccgc catgaccgat tttgtaatca tgaccaacaa aacttcgtac atgtttgtca
     6721 caggtccgga tgtagtgaaa gaagtgctga atcaggatat tacgatggac gaactgggag
     6781 gtggcaacgt acatgccgaa aaaagcgggg tggcacattt tgtatttgaa gatgaagagc
     6841 acgccatcat acaggttcgg aaactccttt catacctgcc gttgaataat ttcgatgccc
     6901 tgcctgtatc caccgatgtt gatttcaacg aagatcgtca ggagtatttg aacaaaatcg
     6961 ttccggatga tccaaaccgt ccgtacgata tgaaagagat aatcaacacg ctggttgaca
     7021 ataactcttt tctggaaatt cacgaaaaat ttgctcaaaa catagtcgtc ggatttgccc
     7081 gtctggaagg aaaaaccatt ggaattgtag ccaatcagcc caaagccatg gccggcacgc
     7141 tggatatcaa tgcaagcatc aaaggtgcgc gtttcgtacg cttctgcgat gctttcaaca
     7201 ttcccctgct tgtactggaa gatgttccgg gattcttacc gggtgtagat caggagcata
     7261 cgggaatcat tcgcaacggc tccaaacttc tttatgcctt ctgtgaagct accgttccga
     7321 gaattactat catcacccgc aaagcctatg gcggagcatt cgtggtgatg aacagcctgg
     7381 gaattggggg cgattttaat tttgcctggc ctaccgccga aatagctgta atgggacccg
     7441 ccggcgctat taaaatagtg aacagagcgg aactgacagc tgcaaccgac cgtgaaaagc
     7501 tggaaaaaga actgattgaa aaatacaaag aagaaatagc caatccgttc aaagccgaag
     7561 aactgggcat gatagacgac gtaattatgc cttcggaaac gcgcaggata ctcgttacgg
     7621 cttttgacat cctgtccaac aagcagtatt cacagcccga acgaaaacac ggtaatattc
     7681 cgctgtaata aaagccccct aaattacccg acgatgtgac tttggagtct gcaatttcaa
     7741 acagaaaaaa tattttttaa gttcatcttc atttattctg aaaaaaaaat caatttcgat
     7801 accatttcga ttcaactata gaacaaaaca aacattacaa tgctctttaa gctacccttt
     7861 ggagaggttt ggagggtctt ttaatttatg aaaaaaatat taattgctaa ccgcaacgaa
     7921 attgccatcc gcattattcg ttctgccaaa caactcggtc tgactacctg tgtgatacat
     7981 tgcaaccgcg aacccgatgc tttgtatctg gagtatgccg atgaaattat ttatgtgaag
     8041 gaagatttga ccggcaagcc gatttttctg aatccggaag caatcgttaa actggcgctc
     8101 gaacatcaga tagacatgat tcatccgggt tacggttttt tgtcagaaaa tccggacttt
     8161 gcagcacttt gtgaagcaaa cggaattatt ttcgtcggtc cttcgcccga attgattcgc
     8221 aacatgggca tcaaaaccat tgccaaggaa atggctgcca gggcagaaat gccactggta
     8281 ccgggtagtc ctggagcaat caccgacgca ctgttagcca aagaatttgc cgataaaatc
     8341 ggatatccgg taattctgaa agcttcggcc ggaggcggcg gacgcggcat gcgcatagtg
     8401 gaaaaaccgg aaaccatgga gcgccacttc aaatcggctc acgaagaagc actggccgct
     8461 ttcggtaacg gcgatatgtt tatagagaaa tacctggtta accctaaaca tcttgaattt
     8521 cagatacttg gcgacaagca cggaaacgta attcacctgg gcgaacgcga atgttcgctg
     8581 cagcgtaagc accagaaaat agtggaagaa gctccgtcgg caagcatcag tgatgaaaac
     8641 agggttaaaa tgggcgaact tgccgtgaaa tttgccaaaa gcatcggata ttattcagcc
     8701 ggaaccattg aatttatact ggatgaagac ggggagtatt attttatgga aatgaatacc
     8761 cgaattcagg tagaacatcc tgtaacagag atgattacag gcactgatct catagcctgg
     8821 caaatacgga tagcgttggg agaaacttta acactgaaac aggaagatat acagttcaac
     8881 ggttgggcca tagaatgccg tgtaaacacc gaagatcctc aaaaccgctt cagtccgcag
     8941 accggattta tcgaaaaaat acatttcccg cacggagacc acatccgtat agaaacggga
     9001 gtaaaagatt tctccgttgt gacgccttat ttcgattcga tgattgccaa aataattgta
     9061 cacggcgaaa atcgtgaaga ttgcatccga aaaacactga ccgctttgaa tgatttttca
     9121 atttcgggct tgaaaaccac tgttccgttt tgtaaaaccg tattgcgaag caaggaattc
     9181 gtggaggcaa cctacaccac gcgctgggtg gatacggtat ttgcaccgga gatgctggaa
     9241 tccgaagacg aagccatgat ggcagccctg gcagctacca tcagctacgc caaggaatac
     9301 ctgcaatact catcggaatc gcccatgttc aaaagcgaat cgctcaatgt gtgggtattg
     9361 aacaagagaa ttaataagta gcccacccaa accctcccga agggaaggat ttttccggca
     9421 agtcaacttt tacagaatta agaatttctt gattacaatt aaaaatatta attaagtttt
     9481 accaacctgc ttattaccac ttcaggcttc gacgtgaact aagccgaacg gaatcaggag
     9541 cacattttta cattatggca acaacatacg tttacaaaag agatgcaacc cggcttttca
     9601 ttgccatgcg cgataacggc agccggtata aaatatacct gcctaaggag tcaaatccca
     9661 ttatcaacgg caaggagaaa gatatttttt tcatcgaaca taaaaataat ttttcgacat
     9721 tcagtatgaa tgacaccttg tatcggtgtg aaatcactca gcgcgaccag aacaggatag
     9781 tggtgaaagt taacggagtg gaatataaat tcagtttgga atcgatattc tcatatttgc
     9841 gtcggaatat gctgaataaa gatgccggtg ctgcgtctga taacaacatc cgttcgcaaa
     9901 tgcccggaaa aatagtagat attttcatga gcgaaggaga cctggtgaat gaaggcgaac
     9961 cgatactgag tctggaggca atgaaaatgc aaaatgaaat caatgcttcc tgcaacggag
    10021 tgattcgaaa aatccatgtc gttcccgggc aatcggtaat gcaggacgaa ttgctggtgg
    10081 aaatagcatc aattgatgtg tgagaaattt agattttttt aaaaaacaaa aaatacccgc
    10141 ttcgaacatt ccatattcgg gcgggtattt ttttcgtttt ttcctttatc agagagtttt
    10201 aagatggata tttctccaga gtaccttgat gccaccacca tcgtgtattt gcagtgcgat
    10261 acgtccctgt gcttttccaa ttttttcatc cgtcagattc accatttcca ccccgttcag
    10321 ccaggttttc acgttatcgc ctgccacacg tattcgcatc gtgttccagt ctttttcttt
    10381 cagaatactc tctttttctt ccggaatctg caccagccag ccgcgtccgt atgattcgta
    10441 gattccgccg gtgtcattac ctcgtggagc cacttccacc tgccagccgt ttactttcac
    10501 atccggttcg ataaacgaac ggaagaaaac gcctgaatta ccatcggctt cctgcttaaa
    10561 ttcgagtgtc agatcaaaat cgttgtaata ttcgcgggtt gccagatagc cgtatttctt
    10621 ttcgggtccg ctttcgcaca ccagcagccc gtcattcaca taccatttct cggtgccgta
    10681 cgcctcccag ccggtgaggt cttttccgtt gaacaaactt accgttttgc tttttcgtgg
    10741 aagctctttg attttcatat tccggaacca agccgccgaa ccgtggtcct gaaggcagat
    10801 tacacctgta catgccagtc cgtattcggg tgcattttcc cattttccgc tgtgtttacg
    10861 cctaaaccag tcatccgtcc aggcttcaaa ttccaccact ttttcgccat tgagccaatg
    10921 ctccacgtga ccgttatcga acaggattcg tgatgtattc cattcaccgg caggtttgag
    10981 cgattttttt gtgtcggcca gatgcatggc gtaatcggca gccgtttttt gccagtcttc
    11041 cagcttctcc ggaaatccga tatcatctat gagctggtat tcaggcccgg taacataagg
    11101 caccttgaaa gccggatttt ccaccacgtg ataaagcatg ccgctgtttc cgccttcggc
    11161 aattttccag tcccaaacga ggtcaaagtt ttcgtagttc tttttggtga ctatatatcc
    11221 gtgagcatcg ccaccgcctc cttttgcctg tatggtaccg tttgccgcaa accagggtgc
    11281 ggtaagcttt tcgccgttgt aatcgcgcca gccgttaagc gttttcccat cgaagagcaa
    11341 ctgccagccg gcgttttttt cttccagagt cagtttgttg tgcgatgttg tggcacagga
    11401 gctcataaaa aaggctgtaa ttccgatgca taataaaaat ttcagtttca taatgatttt
    11461 agtattgatt gtagttaagt atatttatcg tgtaatgatt acagtttctg aacaaaaata
    11521 gagaaataat tctattcgtg aaaaaatcag tatggatttc ctaccctttt attttttgct
    11581 aaaacagctc caaagcctct cccactacga aattacttcc gccgatgtag attaaatcgt
    11641 cggctccggc acgcatcata gccgtcttta cggcttcata tacagtatta tacgtatttc
    11701 catgcagacc gaatgttttt gcctgatttt taagttcttc gggtgataaa gctcttttgg
    11761 tttcagcagc cgtaaagtaa tattccgtat cctttggcag catactcaat acaccggaga
    11821 tgtctttgtc gttcaccatc ccgaaaacga aatgcaattt attgtatttt gtctgttgaa
    11881 gttgctctgt gatgtattca aacccgccct tattgtgagc tgtatcgagt attattttcg
    11941 gattttcact tacagtttgc cacctgccca acaagccggt aagcgatacc acattactca
    12001 atccatcgcg gatatgttgt tcagaaatat tgaaacctgt tttttgtaat tgcttaacaa
    12061 cttccaaaac agttgctaca ttttttagct gataaatacc gttcagccct atttcaagca
    12121 tttcgaaatc ggctgttttt acaatcattt taccattttc gtacctcaga aaacgtaccg
    12181 gctgtaattc ttcagcaaaa aagacaggtg cattttccga ttcagccttt tgaatgaaaa
    12241 ccggtttggt ttctgccaac gattcaccaa ttacaaccgg tgtttccttt ttgattattc
    12301 cggctttttc gaatgcaatt ttatccagtg aatcgcccag aaactgagta tggtcgaaac
    12361 tgatgttggt aatgaccgaa agttccggat gaataatgtt tgtactatcc aaccgaccac
    12421 ccaaacccac ttctataaca gctacatcca cattttcgga ggcaaaataa tcgaacgcca
    12481 tggccatggt tgcttcgaaa aacgaaggtt cgatgcggtc aaaaaggggc ttgtattttt
    12541 ctataaattt caccacaaat tcccggttca ccttcgcacc gttaacccgg attctttcgc
    12601 cgaaatcaac caaatgcggt gacgtataaa gtcctgtttg atagcccgcc gactgaagaa
    12661 tggcagccaa aaaatgagat acagagcctt tgccattggt tccggccacg tgaatggtct
    12721 tgtaggaatt ctgcggattt cccagttcgc tcatcatgcg gatggaattt tccaaaccgg
    12781 gtttgtaagc tgcagctcca atctgatgaa aaaccggaag ccggtcgtac agatattgta
    12841 ttgtttcctg ataatccata gtttgactta cgtgtaaaaa tattctttct cctctgaaca
    12901 aataatgaga catataccag acgcaacaca gcagatttta gatataatca ctaaaagtcg
    12961 cattgtggaa atataaacct tcacgtgtaa atttcagagt atttctttaa ctttgtatgc
    13021 tgatacaaaa atacacaaaa acgatggaaa ccctcaagaa agttcaggaa tttatccaaa
    13081 ccaatgaatt gcttcaaccc agcgagcgga tactcgtagg ggtaagcgga ggtgtagatt
    13141 ccgtagtttt gctgaatatt cttctcaaat cggggtatga atgcattgtg gctcactgta
    13201 attttcattt gcggggcagt gaatctgacc gtgatgaaaa atttgtggaa aaactggcaa
    13261 aagaatacgg tgtcatgttc aaaaaaactg attttaacac caccgggtat gcccgaaaaa
    13321 acaaaatatc catcgaaatg gctgccagag aattgcgcta tgaatggttt aaccgggtgg
    13381 caaaagaaat tcactccgga tcaatcgctg tggcgcacca tgccgatgat tctattgaaa
    13441 cattttttct caacctcgta cgtggtacag gactcaaagg actgaccggc attgacgcca
    13501 aaaacggcaa cattgttcgc cctctgctca cctgcacacg tcaggagata gaaaaatatg
    13561 caaaaatcaa caatcttcag tacgtttttg actctaccaa cgcttcgaat gattacagcc
    13621 ggaataaaat ccgcaatcag gtaattcccc ttttgtcgga tatcaatccg tctgtaagtc
    13681 aaactttcat ggagaacatt atccgactca gaggaatccg gaaaatttat gaagcaaaaa
    13741 ttcagaatat taaagaaaag gtattatctg aaaaagaaga aaatatttac attgatattg
    13801 aggaactgaa gaaacaagct gatattcaga cagtactgta cgaagtgctg aatgagtata
    13861 acttcaacag caaaacaatc agggaaatag tatcgggtct ggaacataat tcaggattgc
    13921 aatatgcttc acccacacac cggctgctca aggacagaaa tgctttaatc atccaacgaa
    13981 tggagcccag aaacgaaaca gaataccttg taagtaaaaa tatccgcgaa ataacagtac
    14041 ctgtacacct tcatttcagg tggataaaaa ataccccgga ttttcagatt tcaaggacgg
    14101 cagacaccgt ccatatcgat gcagacctgc ttgaataccc acttaccatc cggaaatggc
    14161 gaaaaggcga cacatttcag ccgttcggca tgactcaaag caaaaaatta agtgatttct
    14221 tcatcaactc caagctcagt ctttatcaga aagaagaaat ccggctttta ttgtccgctg
    14281 aaaaaatcgt ctgggtcatc ggaatgcgca tcgataatcg attcaggata acagatgaaa
    14341 cacagaatat attagaaatt agttatgaga agtaacgcat atttataaaa taaacgttta
    14401 atttcccatt aaaactttgt taaattcaaa attagttgta cttttgtagc gttttaacga
    14461 aatacacctc cggtcgtccg accgaatgtg ctcacataac aattttcaac aataaaatta
    14521 actttcccgc cagacagcga tagggaaaac tctccatacg tcttattttc atactaatat
    14581 gacaaattat aatattttgc aattaaatgc gaaagaactt tctgaattga aagaaatcgc
    14641 aaacaaaatg gctctgaaag tgccaaactc cataaccaaa gacaaactgg tttacgaaat
    14701 tttagatcaa caggctatca tgaacgccca ggaaaaatcc tcggtttcag aagaaaatga
    14761 aggcaataaa aggaaaagaa tgaggataat tcccaaaaaa gccgaagatc aaaaagtatt
    14821 cagcactgaa aatgtgataa aaaatcaacc tgctgcagaa aaaacagaaa aagtagtaaa
    14881 acctgaggcg gaaaagacag aaaagtcaga taaacccaaa cagcaaccgc ctaaaactga
    14941 aactccgaaa gcagacacca ctccggcagc aaaacaggaa actaaaaaga aaacttcgcc
    15001 aaaagccaaa aaaactcctg ctaaaaaaga agcacctacc gcagtaaaag tggaagaaac
    15061 acctgtggac acaaaagcag aagcagtaat agccgaagaa cagaaggaaa caaaaatagc
    15121 accgcctgtc aggaaaatac tgcaacccgt caaagaggag atcaaaaccg tgatagacga
    15181 tcaattgctt caggaaatca tcaatgaaga agaaacgaat gaaactgata ttgccactga
    15241 aaaagaagag caaccacagg gcactcacaa gcatcaaaga gaaaatcagg gcaacggaaa
    15301 ccgcgtaaaa ttcaacccac gccccgaaaa acaattcgat ttcgaagaag tgctggaagg
    15361 caccggaaca cttgaaatcc ttactgatgg atacggtttt ctccgttcgc ccgactacaa
    15421 ctacctttca tcaccggatg acatatacgt ttctcaatct caaatcaaac ttttcggtct
    15481 gaaaaccggt gatacggtga aaggatccat tcgccctcca aaagaaggcg aaaaatactt
    15541 cccgttgatt cgggtttcga aaatcaacgg ccgtgacccg gcttacgtac gcgaccgcgt
    15601 acctttcgaa cacctcgttc cccttttccc cgaagacaaa ttcaccctga acagcggaaa
    15661 aaacgacaca ctttccaccc gcattgtgga tctgttttgc cccataggaa aaggacaacg
    15721 cggactcatc gtggctcaac ccaaaaccgg taaaaccgta ctgctcaagg aaatagccaa
    15781 tgccattgct gccaatcatc cggaagttta catgatagta ctgctcatcg acgagcgccc
    15841 ggaagaagta actgatatgg cacgaagcgt acgggccgaa gtaatctcct ccactttcga
    15901 tgagccggcc gaacgccacg tgaaagtagc cagcatggtt cacgaaaaag caaaaaggat
    15961 ggtagaatgt ggacaggatg ttgtcattct gctcgactcc attacccgtc tggcaagagc
    16021 ttacaacacc gtttcgcctg cctccggcaa agtactttct ggcggtgtgg atgccaacgc
    16081 actccacaaa cccaaacgat tctttggtgc agcacgtaac attgaaaacg gcggaagcct
    16141 caccatcatc gctacagcgc tcactgaaac cggatcgaaa atggatgaag tgattttcga
    16201 ggaattcaag ggaacaggca acatggaatt gcagctcgac cgcaaacttt ccaacaaacg
    16261 cattttcccg tcggttgaca tcatggcatc aagcacacgt cgcgatgacc ttctgctgga
    16321 taaagacaca ctcaaccgta tgtggattct gcgtcgccat cttgccgaca tgaattcaat
    16381 ggaagcaatg gaattcctca aagaacggct cgaacgaact gaaaacaatg aagaatttct
    16441 gatttcgatg aaccgcgaat agccgaaaat tcaataataa aaaataatag cttgccctaa
    16501 aattccggca ggctattttt ttttaatgaa tgtttgatat tacacctaac ttagttatca
    16561 attggggctt tagtccataa ataaataaac ttcggcaaaa gccttttgtt tcttaaatca
    16621 agaatccatg gcttaaaagc tatggcaatt aattttggta aagagtgcga taatcatttt
    16681 tattttcata taaatgtatc aattccggtt gattggtatg aaaatttaaa tttacggcaa
    16741 agatgaaacc tgtaaaatca tgcttccatc caatgcttca taaaatcatc gttactttga
    16801 gcaaatgttg gtttggagta actgtcttct atggtaggct gaatcggaaa ccgcctgaaa
    16861 tcccagtcat ccggagagtt gaaataataa taagcaagcc ggttggccga ccattctgtc
    16921 catgtatgct gatgtttccg gtgttctttt cgcgaagcgt gagcgctgtc caggctcgtt
    16981 ttggcaatat ggcgccagaa aaaccaaaac ccccatttcc ggaactgcaa aatatgcccg
    17041 aattcatgac gaagcaaatt aaggtcatct tccatgcctt ttcctacaaa tattccaacg
    17101 agaggaatgg taagtgccgc tccgcgcctg aagttgccga agttgagcct gaataatcga
    17161 acttgctgaa accgcatcat tgtcagtttt tgaaaatttg aaaaaaataa attataaatt
    17221 gattgaaatg ctgtaatttt gacacataaa aaaaatcttt tttcatggat gcgaccaaat
    17281 tctacaccaa acgggaagaa aaagccaact ggataactca ccttttgggc attatgatag
    17341 ccattgccgg cacatattta ctcacagcca aagccattgt tcaccaaaat atttgggctc
    17401 ttcttgcatt tttaattttc ggctttggca tgttcgtttg catgcttgca tccacgctgt
    17461 atcactatgt tcagacaccg aaaacgaaat cactgcttcg ccatttcgac cacggaagta
    17521 tctatgtatt aatcgcttgc agctattctc cttttactct cattttgctt cgcaatgagg
    17581 ggatctgggg ttggggactt ttcgtgctcg tgtggattat tgccctaacc ggtatcggat
    17641 ttaatttcgg caccttgaaa gccaatagtc atctcaaaac tgcttcctac gttttgatgg
    17701 gaatgattat cctcattgcc atcaagccat ccattgaagt agctgcttcg ctcggctgca
    17761 tgaatgtact gctttggctg ctcatcggtg gcattttcta catcgccggt gcagtaattt
    17821 atgccctggc gaagcatgaa tttatacatg ccgtttttca tgttttcgta ttgctgggca
    17881 tgatgagcca catttacgca gcatatttaa taccgcttta atcctgtttt ttaggacgtc
    17941 ttattacatt ttcgtacgta tcaatccagg tctgaacatc cactttccgc gccagttcgc
    18001 caatcagttc atatggaata tcatccattt ttttaaaacg aatacaccct ttgcccatat
    18061 ccggttttgt tttgcaatgt ttgggatact cagcttcaaa ccactcagca attccgggaa
    18121 ccgaatatac gcccatatga tacaacgcaa catggttttt ctgtgaagcc agattcatca
    18181 gcgggagcgg tactttgggt ttcacagcat agcccgccgg ataaatggaa aatgggatga
    18241 catagccgat cattccataa cttatttctt cttcataacc gatcggaaga ttctctttca
    18301 acactttacg cagtttttcg atgactattt ttcggtcgtc ggaaagtccg gcaacataat
    18361 catcacatgt ttggggattt gtagtcatgg gtggttttta tattaaagtt ttaatttcag
    18421 acaagtttta cttcagatca gatgcaaaaa taatatattt gttgaaatgc caaaagttaa
    18481 tgatgtttca ggaaaatttt attcaaaaaa aaacctgcca aagttcataa ctttgacagg
    18541 attcatattt tgcgtttcct taactgatta agaataaatc tccccttttg ctgccaacaa
    18601 cgtatttttt agcagagaaa caatcgtcat gggtcctact ccacccggta caggcgtgat
    18661 gtaagagcat ttcggtgcta cttcgtcgaa tttcacatcg cctttcaggc ggaaaccgct
    18721 tttggtttcg gtgctgggaa cgcgcgtggt acccacatca atcactacgg caccttcctt
    18781 caccatatcg ccccgcaaaa actcgggttt tcccaatgct gcgataatga tatcggcttg
    18841 cagcgtaatt tctttcagat tttgggtgcg gctgtgacaa acggtaacgg tagcatcgcc
    18901 cggatatgat ttttgcatca tcagcgaagc aattggtttt ccaacgatat tgctgcggcc
    18961 aatcaccaca caatgttttc cggaagtcgg gatttcgtag cgtttcagca gttccataat
    19021 tccataagga gtagcagaaa cgtagcaagg taacccgatg gacattctgc caacatttac
    19081 agggtgaaat ccatccacat ccttgcggta atcaatg
//
LOCUS       DAORKP010000026        19028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_148_length_19028_cov_8.992463, whole genome shotgun sequence.
ACCESSION   DAORKP010000026 DAORKP010000000
VERSION     DAORKP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148_length_19028_cov_8.992463"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLN75_02730"
     CDS             complement(<1..267)
                     /locus_tag="PLN75_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03064.1"
                     /translation="MLLILLHNISTASSLLKSGYQLARNLKKEVGVLAFVESAEEVNA
                     KKENLAKYFSENGFDDFQVFVRPKSVKHLAEECEAVEASFLMMQW"
     gene            complement(463..2919)
                     /locus_tag="PLN75_02735"
     CDS             complement(463..2919)
                     /locus_tag="PLN75_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HRG03065.1"
                     /translation="MKLSKIAQLLGFPVIDDADAEISWLLTDSRSLSFPSGTLFFALK
                     TSRNNGHKYIPELYRQNQRFFVISEILPDFENLTDAVFIQVNDTLQALQTVAAAHRAE
                     FGIPVVGITGSNGKTVVKEWLYQLLHSDFRIVRSPRSYNSQIGVPLSVWPLSESTGMG
                     IFEAGISQTGEMEKLQPIICPTVGIFTNLGDAHQEGFSGLKQKADEKLKLFKEAEVLI
                     YNSDDKLLEIAIMQAKLKAGLFAWGKKPDARLRVLQVVKNGNTTISLKYEKQHFDVEI
                     PFTDDASVENALQCVCVMLYLNYTPELIAQRLLRLETVAMRLEVKEGVNGCLIINDTY
                     NSDLNSLNIALNFLNLQATRKNLSRTLVLSDILQSGHTSAELYSMVAELVKNKQINRM
                     IGVGPEISANTSLFETPEKYFFRDTADLLTSPLMREMRNEIILLKGSRSFHFEDLLAR
                     LEQTVHETTLDVNLNALVSNLNYFRSKLRPETRVMCMVKAFAYGSGSVEIARTLQNNR
                     CEYLAVAVADEGAELRREGIRVPIVVMNPEKSAFNTIFDNKLEPEIYSFRLLKLFIEA
                     AEKLALTDYPIHIKIDTGMKRLGFEEKDMPELLNLLKNQNQVKVRSVFSHLVGSDNPA
                     FDDFTRGQIELYSRCADVISTSFSHRILRHILNSGGIERFTEYQFDMVRLGIGLYDVS
                     ALPGVKLETVCTLKTIILQIRDVKAGETVGYSRVGKLTRDSRIGVIPIGYADGFDRHL
                     SNGAGEVWINGHRARVVGNISMDITMIDLTGIPAEEGDVVEIFGQNITIQEVADKLET
                     IPYEILTSVSRRVKRVYFVD"
     gene            3157..3591
                     /locus_tag="PLN75_02740"
     CDS             3157..3591
                     /locus_tag="PLN75_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HRG03066.1"
                     /translation="MSTVNPTMLLRDETVLPTNEVIKKVLGEELFGVYSGLITSFTSE
                     LDLEPQWRYYNDGKAWLCKVVYRKKTILWLSLWQDCIKTSFYFTEKTLPGIMNLDIHA
                     SIKELTGNAAHIGKLIPLILNISRSEQLNNLKKIIQYKKESK"
     gene            3820..5373
                     /locus_tag="PLN75_02745"
     CDS             3820..5373
                     /locus_tag="PLN75_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG03067.1"
                     /translation="MKKNLLLAMLVLSTFAMAQFNQRMNPAKQAEKTPMEIIKSFPDK
                     KTWKTPGTITAQKINELINAQQTMAAEYQLMDSIINWNLDLSNGEWKNSDKTIKMTYD
                     NKNRLISLIVQNWDLDTQEWVNSGKSTYTYNNNGDVTQLLIEGWDEGEWINTMKSTMN
                     YDRNNNLINSKIELWMDEWIALSYTEFTYDNNHNVLTEINQAGLFGPLKNETKEIFTY
                     DTKNNVLTSIHQVWNGEDWENQSKITNTYNAKNYILTYTNQTWESGTWLNFNKQTFTY
                     NSNDDETLWLSQMWKSDNWENIAQHISTYDTRQNLTTQIQQSWENGAWVNYQKYAYTY
                     NNSDLQLTELVQEYRENAWLNVVLSTSTYDTRGNNTSETTQIWLDNAWYRTGLTQETY
                     DMNNYNITYVNWGFDMDGETILSGDSSRFYYHPVSSGIPETIDEQLTVYPNPVKDYLI
                     LKIDAASSLTGEMMSYSLFDLSGKLLESRKLEGNVTRISMRRLPPATYVIKIYDGNKT
                     RKEFKIIKM"
     gene            complement(5604..6089)
                     /gene="tpx"
                     /locus_tag="PLN75_02750"
     CDS             complement(5604..6089)
                     /gene="tpx"
                     /locus_tag="PLN75_02750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRG03068.1"
                     /translation="MATTNFKGNPVKTNGELPKVGDKAPDFKLVKGDLSEVTLADFAG
                     KKLILNIFPSIDTGVCATSVRKFNQIASEKGITVLCVSKDLPFAASRFCGAEGLNDVI
                     TASDFRYNNFATDYGVLMEDGPLKGLCARSVVALDENGVVTYAELVPEITIEPDYNVP
                     V"
     gene            6220..7407
                     /locus_tag="PLN75_02755"
     CDS             6220..7407
                     /locus_tag="PLN75_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03069.1"
                     /translation="MKKQLPYSIIIFVSVSFFSCQDWNEYRVKSDFEPYVVRFEMEAK
                     QRGEDFHLRERGLIIEFGDLDNGVAGLCHYGKPIRIEIDRAYWNEMGKYGGADLIREE
                     LIFHEMGHGILGRKHLNTTLSNDEWKSIMCGGNKVEDRSWNTNYRGQRRAYYLDELFT
                     ESTAEPSFFTLPPVIDSEAFIPVLTDNFDSASSSLWKPGIYPGSSVSIENGMLIYRSK
                     STANMAVLSEIPHFNIQSDFILEYTFQYKEQADSAKYGIAFGTLANSGSYKESVDFFV
                     INNNRQLYNGNTTWYSYFTQVKRYQIIPKGKNKLKIIQSGNMLSCYINDVFAYASETE
                     NTQPGSGIGFMVPAGQTLYVDDFRLSKRTTAAAAARIRKSLTDKLNFTPVMSIINEIK
                     NNK"
     gene            7419..9110
                     /locus_tag="PLN75_02760"
     CDS             7419..9110
                     /locus_tag="PLN75_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRG03070.1"
                     /translation="MPKFVIYQVLPRLFGNYNTTRKYKGTIEENGCGKFNSFTPRALE
                     EIRKLGATHIWYTGILEHATKTDYSAFGIRKDHPGVIKGNAGSPYAIKDFYDVDPDLA
                     ENVTNRMEEFEALIRRTHEAGLKAIIDFVPNHTARQYHSDMKPNGVKDIGENDNPDWA
                     FSPLNNYYYIPNEQLRPMFNTEGYDEYPAKATGNDQFTAYPSEFDWYETVKLNYGVNY
                     MEGHQKQFDPIPDTWHKMADILCYWAAKNIDGFRVDMAEMVPVEFWGWAIPQVKNINP
                     EIIFIAEVYKPAEYNNYLCNGKFDYLYDKVGLYEKLRGILSRNEAARDITGIWQSLSR
                     ISDRMLNFLENHDEQRIASGFFCGSGTYAEPAMIISATLTKAPVMIYMGQELGELGMD
                     MEGFSGVDGKTSIFDYWGVSSLQAWANNGEFDGANLTDEQWNIRNCYQKLLNICLSEK
                     AITEGEMYDLEFANFGNPAFNTHEQFAFIRKHRNEILLIAVNFYDKPVELEINIPYEA
                     FQYLKLHDGDYYSVQNLLNEEEDLPIQRLTSYIPYRVNLSAWNGKIMKLKKANVV"
     gene            9307..10587
                     /gene="eno"
                     /locus_tag="PLN75_02765"
     CDS             9307..10587
                     /gene="eno"
                     /locus_tag="PLN75_02765"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRG03071.1"
                     /translation="MSKIEKIVAREVLDSRGNPTVEVDVTLESGVVGRASVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVENVNKVIAPALIGECALQQRKIDKAMIALDGTKT
                     KSNLGANAILGVSLAVAKAAANYLDMPLYRYIGGTNTFTLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGACCFREGLRMGAEVFHSLKKVLHDKNLSTAVGDEGGFAPNLAGGTEE
                     ALESILLAIKNAGYEPGKDVMIGLDCASSEFYKDGIYDYTKFEGANAKKRTSAEQVDY
                     LAELVANYPIDSIEDGMGEGDWDGWKLLTDKLGKKIQLVGDDLFVTNVEYLKKGIDMG
                     CANSILIKVNQIGSLTETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQI
                     KTGSLSRSDRMAKYNQLLRIEEELGDLAAYGWKK"
     gene            10887..11438
                     /locus_tag="PLN75_02770"
     CDS             10887..11438
                     /locus_tag="PLN75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRG03072.1"
                     /translation="MNRTGERIKKKREQFNLQLNELAEKIGITSSALSQIEKGKSFPS
                     ILTLKNIADKLHTTVGELIGENDSQANNPVVYKSDIKFIDQSKSGTMFYLLSQHDVNK
                     QMDTYLVRFSKASAIDGFFSAAYGQIFSYVLAGEVRFDLDGKTFILKSGDNIYFNAKS
                     QFNAINYFDGVSEIVWVQSPPSY"
     gene            11512..12057
                     /locus_tag="PLN75_02775"
     CDS             11512..12057
                     /locus_tag="PLN75_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRG03073.1"
                     /translation="MTKYPAVFLDRDGTLIDDVGYIRHTSEVSFYPFTSEALLMLQEH
                     FLLFVITNQSGIAKGLTTEKEVSEVNKYILNTLKKQDIIIHDLFCCPHKNEDNCECKK
                     PKTQFIEKAATKYDIDLTRSFIIGDHPSDVQCGINAGITPLYLLTGHGRKHKGEISNE
                     TIIYTNLLEASKYIINKNKQT"
     gene            12054..14009
                     /locus_tag="PLN75_02780"
     CDS             12054..14009
                     /locus_tag="PLN75_02780"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HRG03074.1"
                     /translation="MNDLLTVLREKNIIVPDNIAELLTGCKSYKVFNTTEELSEAATN
                     GKENEQFEVQYDVPGKGIYTEAIVQRVKNGISANYTESYMRRRDPDTMVIADNNPTDK
                     IRFINKYGYEFSGLQTDTFEWLKQQDLAVFFYFAGRENIGSLGIAIAPANAAFFALGL
                     SMLQEMVAVDELKDNAELKSVIFVAPVFRHTHFNGKQVVVHNRTESIHELYSYNLYPG
                     PSAKKGLYGVMLNQAEKEDWIIAHCSAVQSVSPYDNLTTFMHEGASGGGKSEMHQHIL
                     RETDGRVSLGRNLITSEERFITIPRFCTFNAVADDMAFCHPSVQKKDGKLRIIDAENA
                     WFVRVDSVKQYGDDPSLEKTTINPTSPLLFLNIETTPGSTALIWEHIQDKPGKSCPNP
                     RVIIPRKTVPNVVNRPVSVDIRSFGIRTPVCSKENPSYGIIGMFHVLPPALAWLWRLV
                     APRGHNNPSIVSSGGMESEGVGSYWPFATGQRVPQANMLLKQIIDTPRTTFTLVPNQN
                     IGVWQVGFKPQLLMREYLTRRGAAQLKTDQYQAARCPLLGYELNYLTLEGSKIPSRFL
                     KVYNQPEVGFEGYDAGAEMLRAFFKSELQKYLKEDLLQTGKRIIEACLSDASIEDYNE
                     IVPMSYQYEYSFNKIEDYEQSSDNPGF"
     gene            14119..15078
                     /locus_tag="PLN75_02785"
     CDS             14119..15078
                     /locus_tag="PLN75_02785"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRG03075.1"
                     /translation="MTNNNISEAAQIIKDGGLVAFPTETVYGLGANALNPFAVAKIFE
                     AKERPSFDPLIVHIASFDDLNILSKGINENVIRLAKHFWPGPLTIVLPKSSVVPDIVT
                     SGLNTVGIRMPDNPIALELIKQAGCPIAAPSANKFGLLSPVNAKHVRKQLPGIGYVLD
                     GGNTRVGIESTIIAIDGHTCQLLRPGIITISDIEKAFPDTFVFNNSKPDKLVSPGLLK
                     SHYSPRKPLYFLDNRLDFPEFSGLILHCETNTCYNAQRVIYTSRTRDKIEITANLFAS
                     LHAMEEDDSIKNIFIEPVEETGLGIAIMDRLKKAAYRYNDRMD"
     gene            complement(15367..15783)
                     /locus_tag="PLN75_02790"
     CDS             complement(15367..15783)
                     /locus_tag="PLN75_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HRG03076.1"
                     /translation="MKRIVYLFAAVTMILFTAGCSGGNAGKSPKDIEKAIYNELQKGN
                     YEKAVEMLFENVESDDDTPAEQKAEVMKAFTEKTKQSADAKGGLKSFEILEEKIAEDG
                     KTATVTTKVTYGNGTEDTQNSTYVNKDGVWKLNMNK"
     gene            complement(15816..16442)
                     /locus_tag="PLN75_02795"
     CDS             complement(15816..16442)
                     /locus_tag="PLN75_02795"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRG03077.1"
                     /translation="MILKMKLLPVVRVMLMLLLLSLLTVCCERAETNRFASVKLEEGD
                     LVFRRGTGAKSRAVMQADSLGIYSHVGIAVRRDESFGVIHVTPGERQKGDTVDRVKME
                     SLDEFWSTARAVHGAVYRLKDSKTGAMASSQALRLLDKGILFDHDYLLNDTTQMYCTE
                     LVWCAYSLAGVDISSGRRSRINMPVFGGMYIFPSDIYLNPQFELVFSF"
     gene            16903..18216
                     /gene="der"
                     /locus_tag="PLN75_02800"
     CDS             16903..18216
                     /gene="der"
                     /locus_tag="PLN75_02800"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRG03078.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTKSRRAIVNEQAGTTRDRQYGK
                     SEWEGREFSVIDTGGWVVNSSDVFEEEIKRHVMLAIEEADVILFVVDVQNGLTDLDEA
                     VAQILRRSEKPIMLVANKTDTFDLQYQAAEFYKLGLGEPFILSSINGLGSGEMLDELL
                     KHFKSEATDETEEELPRFAVVGRPNAGKSSLINALIGEDRTIVTDIAGTTRDSIYMRF
                     NKFGHDFYLVDTAGIRKKAKVNEDLEYYSVVRSIRAIENSDVCILMLDATRGIESQDL
                     NIFSLIQKNKKGLVVVVNKWDLIENKEANDTKIFEKSIRERLAPFTDFPIIFTSVPNK
                     QRILKVLDTAAKVYENKKRKIATAKLNEVLLPIIEKSPPPALKGKYIKIKYITQLPNT
                     FIPTFVFFANLPQYVKEPYRRFLENKIRDKWELHGTPINVFIRHK"
     gene            complement(18281..>19028)
                     /locus_tag="PLN75_02805"
     CDS             complement(18281..>19028)
                     /locus_tag="PLN75_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRG03079.1"
                     /translation="YVTTDFGSSVMVNVNNLSLTLYYHYVYKNDPAQAKIKSYHTFPA
                     NAEIVKVNRVQHPLRTLSSGPDDEFNYIASPANYHTKVHIPIGRIRQRIKTGGKQLDV
                     NSAILKVNVQDRTKWGTSALIPYVSGLLLIRERDLDKFFTNHELPVDTASFVATLGYE
                     NITATTYKYNYTFSSLNNLIENELKKNSTDEYLDMVLVPVSLVQASSSSYSQSVLTEV
                     NQSTQMQAVSIFSGKNKDIPMKLEVVYSGF"
BASE COUNT         5891 a         4194 c         3892 g         5051 t
ORIGIN      
        1 ccattgcatc atcagaaatg aagcttctac agcctcacat tcctccgcca gatgcttcac
       61 cgattttggc ctcacaaaaa cctgaaaatc atcaaatcca ttctccgaaa agtattttgc
      121 caaattttct ttcttcgcat tcacttcctc cgcactttcc acaaaagcaa gtaccccgac
      181 ttcttttttc agatttcggg ctaactgata tccgcttttc agaagactgg aagcagtgga
      241 aatattgtga agtaaaataa gaagcatctg tctgaactat gattttagaa tgattttggg
      301 atttataaga tttagttttt tttgattaaa aaaccttttc agtaataatt aaaacatcct
      361 ctgttttaag aataatctga tttattttta atagaattgc atcccatttt tagcatattt
      421 tcttaatcaa tctaatcata aaaatcacag ttcagacttt tcttaatcca caaaataaac
      481 ccttttcacc cgtctggata cgctggtgag aatttcgtaa ggtatggttt ccagcttatc
      541 ggccacttcc tgaatagtaa tgttttgtcc gaagatttcc accacatcgc cttcttcggc
      601 aggaataccg gtcaggtcaa tcatcgtaat gtccatactg atattgccga ccaccctggc
      661 ccgatggccg ttgatccaca cctcgcccgc tccgttgctc aggtggcggt caaatccgtc
      721 ggcatagcct attggaatga ctccaattcg gctgtcgcgc gtaagttttc ctacccggct
      781 gtagcccacg gtttcgcctg cttttacatc ccttatctgg agaataatgg ttttcagcgt
      841 acacaccgtt tcgagtttta ctcccggcaa agcacttaca tcgtagaggc cgattccgag
      901 ccgtaccatg tcaaactgat attcggtgaa acgctcaatg cctcccgaat tgagtatgtg
      961 tcttaaaatc cggtgactga acgatgttga aatcacgtcg gcacagcggg aatagagttc
     1021 aatctgaccg cgcgtgaaat catcgaacgc aggattgtcg gagcccacga ggtgcgaaaa
     1081 taccgaccgt actttcacct gattttgatt tttcagcaga ttgagcagtt caggcatgtc
     1141 tttttcttca aaaccgagcc gcttcatgcc cgtatctatt ttgatatgga tcgggtaatc
     1201 agtcagcgcc agcttttcag cagcttcaat aaacagtttc agcagccgga aactgtaaat
     1261 ttccggttcg agtttgttgt caaaaatcgt attaaaggcc gatttttcag gattcattac
     1321 cacgattggc acacgaatgc cttcacggcg caattcggct ccttcatcgg ccacagccac
     1381 ggccaggtat tcgcaacggt tattttggag cgtgcgggct atttccaccg atccgcttcc
     1441 gtaagcaaat gctttcacca tgcacatcac gcgggtttcg ggtctgagtt tggaacggaa
     1501 atagttcaga ttgctcacca atgcattcag attgacatcc agcgtggttt cgtgaacggt
     1561 ctgctcgagt cgtgccagca aatcttcgaa atgaaaactc cgtgatcctt tcagcaggat
     1621 gatttcattt ctcatttcgc gcatcagcgg cgaagtcagc aaatcagcgg tatcacggaa
     1681 aaaatatttt tcgggagttt cgaacagact ggtgttggca gaaatttccg gccccacgcc
     1741 gatcatgcgg ttaatctgct tatttttaac cagttcggct accattgagt agagttcggc
     1801 cgatgtatgt ccgctctgca gaatatccga gagcacgagt gtgcgcgaaa gattcttgcg
     1861 cgtagcctgg agattcagga aattgagcgc aatattgagc gaattcaggt cggaattgta
     1921 cgtatcgttg atgatgaggc agccgtttac gccctctttc acttcgaggc gcatggctac
     1981 cgtctccaga cgaagcagtc gctgcgcgat tagttcggga gtgtaattca gatatagcat
     2041 cacacagaca cactgcaggg cattttccac cgaggcatcg tcggtaaaag ggatttcaac
     2101 atcgaaatgt tgtttttcgt atttcagcga aatggtcgtg ttgccatttt tcactacctg
     2161 cagcaccctg agtcgggcat ccggtttttt cccccaggcg aaaagtccgg ctttcagctt
     2221 tgcctgcatg atggcgattt cgagcaactt gtcgtccgaa ttgtaaatga ggacttccgc
     2281 ctctttgaaa agtttgagtt tttcgtcggc tttctgcttc agtccgctga aaccttcctg
     2341 atgtgcatcg ccaagattgg taaaaatccc taccgtcggg caaatgatgg gttgcagttt
     2401 ttccatttcg ccagtttgtg aaatgcccgc ttcaaaaatg cccattccgg tactttcgct
     2461 cagtggccag accgacagcg gtactccgat ctgggagttg tagcttcgcg gtgaacgtac
     2521 aatcctgaaa tcggagtgca gcaactgata gagccattct ttcaccaccg ttttaccgtt
     2581 gctgccggta atacccacta ccggaatgcc gaactcagcc cggtgagcgg cagctacagt
     2641 ctgcaaagcc tgcaacgtat catttacctg aatgaataca gcatctgtga gattttcaaa
     2701 atcgggaagt atttcactga ttacgaaaaa gcgctgattc tggcggtata attcgggaat
     2761 gtatttatga ccgttgttcc ggcttgtttt tagggcaaaa aaaagcgttc ccgacgggaa
     2821 tgacaacgaa cgactatcgg taagcagcca gcttatttcg gcatctgcat catcgataac
     2881 cggaaaaccc agcagttgag ctattttaga aagtttcatt ttgtgtaata attgaaatgc
     2941 aaagttacag ttttttttca gacatagatt caactctttt ctgcgaagaa tgaatgagac
     3001 tgacagctga tgtaaaaaaa tatcaaaaag ttttgatttt ttataaaata acatctaatt
     3061 ttacaaccgg ttgctgaaat agttgaaagt gcttgagaaa aagtgtttca actattccgt
     3121 caatgatttg gcatgtataa aaatttcaaa aaaaagatgt caactgtgaa cccaacaatg
     3181 ctccttcggg acgaaacggt gctccccacc aatgaggtaa taaaaaaagt gctcggcgaa
     3241 gaacttttcg gagtgtattc cgggctgata acttcattca cctctgaatt ggatctggag
     3301 ccgcaatggc gttactacaa cgacggcaaa gcatggctgt gtaaagtcgt ttaccgaaaa
     3361 aaaacgattt tgtggctttc gctgtggcag gattgcatca aaacgagttt ctatttcacc
     3421 gaaaaaacac tgcccggtat aatgaacctg gacattcatg cttctataaa agaactgacc
     3481 ggaaatgctg ctcacatcgg caaactgata ccgctgattc tgaacatatc aagatccgaa
     3541 caactgaata atctgaaaaa gataatccaa tacaaaaagg aaagcaaata aaatctgttc
     3601 aaaaaaaacc ggacagtttt ttacgatatt ttttttgaaa cccccttttt ggggtagcct
     3661 tacaaaaaaa ggaacactat ttttgcatca aaagaaaaag gaaagattcc gaccgtaatt
     3721 ctcgtctttc gtctctatga ttgataaaaa caagaaaaaa tcgtgaatga agcatacaca
     3781 attaacaaaa aacataatca ttcaaaaaaa caccccgtta tgaagaaaaa tctactactt
     3841 gcaatgttgg ttttatcgac ctttgcgatg gctcaattca atcaaaggat gaatccggcg
     3901 aaacaggccg aaaaaactcc gatggaaatc attaaatcat ttccggataa gaaaacctgg
     3961 aaaactccgg gcacaatcac cgcacaaaaa atcaacgaac tgatcaacgc acagcaaaca
     4021 atggctgcag agtaccagct gatggacagc attataaact ggaacttgga cttatcaaac
     4081 ggagaatgga aaaacagcga caaaaccatc aaaatgactt acgacaacaa aaaccgactc
     4141 ataagcctga tagtgcagaa ttgggatttg gatacgcaag aatgggtaaa ctccggcaaa
     4201 tcaacctata catacaacaa taacggcgat gtaacacagc ttctgataga aggctgggat
     4261 gaaggagaat ggataaatac aatgaagtca accatgaact acgatagaaa caacaacctg
     4321 attaactcaa aaattgaact gtggatggat gagtggattg cattatctta caccgaattt
     4381 acctatgaca acaaccacaa tgtgctgacc gaaatcaatc aggcagggct tttcggaccg
     4441 ttgaaaaatg aaacaaaaga aattttcacc tacgacacaa aaaacaacgt gctgacttcc
     4501 atacatcagg tgtggaacgg tgaagactgg gaaaaccagt caaaaatcac aaatacctac
     4561 aatgccaaaa attacattct gacatacacg aatcaaacct gggaatcggg tacctggctt
     4621 aatttcaaca agcaaacttt tacatacaat tcgaacgacg atgaaacact ttggcttagt
     4681 cagatgtgga aatcagacaa ctgggaaaac atcgcacagc atatctccac atacgacacc
     4741 cgccagaacc ttacgaccca gatacaacag agctgggaga acggtgcctg ggtaaactac
     4801 caaaaatacg cttacacgta caacaactcc gacttacagc taaccgaact ggtacaggaa
     4861 tacagggaaa atgcatggtt aaatgtagtg ctatcaacaa gcacttatga tacacgcgga
     4921 aacaacacca gtgaaaccac tcaaatctgg ctcgacaacg cctggtacag aaccggactc
     4981 acgcaggaaa cttatgatat gaataactac aacatcactt atgtaaactg gggatttgat
     5041 atggacggag aaaccattct ttcgggcgat agtagccgct tttattatca ccctgtttcc
     5101 agcggaatac ccgaaactat tgatgagcaa ctaacagttt accccaatcc ggtgaaggat
     5161 tatctgattc tcaaaatcga tgctgcatct tcacttacgg gtgaaatgat gtcttacagc
     5221 ctctttgacc tcagcggtaa gctgcttgaa agccgtaaac tcgaaggaaa tgttacccgg
     5281 atatccatgc gcagactgcc gccggcaacg tatgtgataa aaatatacga tggaaataaa
     5341 accaggaaag aattcaaaat tattaaaatg taaaaaatcc gacggtactg accggatagt
     5401 taactacccg accgaaaccg tatagaaaac ctgaacctga aagaaatacc ttgtctgata
     5461 ccgacagggt atttttgatt ttcagggaat aaccggggaa atttatctgc aaaaaaaata
     5521 taccggaaat gtttttaaaa caaaaaaaag acctgctttc agaagcaagt cttttattct
     5581 acttttaaac acactttttt ttgctatacc ggtacgttgt aatcgggttc gatggtaatt
     5641 tccggtacga gttcggcgta ggttaccact ccgttttcgt cgagcgctac caccgagcgt
     5701 gcacaaagtc ctttcagcgg tccgtcttcc atcagtactc catagtcagt tgcgaagtta
     5761 ttgtaacgaa aatcggaagc cgtaattaca tcgttcagtc cttcggcgcc gcagaaacgt
     5821 gatgccgcaa aaggaaggtc ttttgatacg caaagtaccg taattccctt ttcagaagct
     5881 atctggttga acttgcgcac cgaagtggca cacacgcccg tgtcgatact tgggaaaata
     5941 ttcagaatca gtttcttacc ggcaaaatca gccaaagtaa cttcacttaa atcacctttt
     6001 accagtttga aatcaggagc tttatctcct accttaggca attcaccgtt ggttttcacc
     6061 ggattgcctt taaaatttgt tgttgccatt tgtagatatt ttaaatttat taataattta
     6121 tcttataata attactacat acaacaatgc tttgcggttt tttgtttata tttgtagaaa
     6181 agtttaacta cgacaagcag ccttttgccc gaaaagtttg tgaaaaaaca gcttccatat
     6241 agtataatca tttttgtaag tgtttcattc ttttcatgtc aggactggaa cgagtatcgg
     6301 gtaaagagtg attttgaacc ctatgttgtt cggttcgaaa tggaagcaaa acagcgggga
     6361 gaggattttc accttcgtga gcgcggactg ataattgaat ttggagacct ggacaacggc
     6421 gtagccggat tgtgtcacta cggcaaacca atccgcattg aaattgacag ggcttactgg
     6481 aatgaaatgg gtaaatacgg cggtgcagat ctgatacgcg aagaacttat atttcacgaa
     6541 atgggacacg gcattctggg acgaaaacac ttaaacacaa cactttcaaa cgacgaatgg
     6601 aaatcaatca tgtgtggcgg caacaaagtt gaagaccgtt cgtggaatac aaactaccgc
     6661 ggacaacgcc gcgcatatta cctggacgaa cttttcacag aaagcactgc agaaccgtcg
     6721 ttttttaccc ttccgcctgt aattgactcc gaagctttta tccctgttct gactgataat
     6781 tttgacagcg cctcctcttc actctggaaa ccgggcattt atccgggttc ttcagtttcc
     6841 atcgaaaacg gcatgctgat ataccgctcc aaatcgactg ccaatatggc tgttctttca
     6901 gaaatacctc atttcaacat tcagtccgat ttcatactgg agtacacctt tcagtacaaa
     6961 gaacaggctg acagcgcaaa atacggtata gctttcggca ctttagccaa ttccggctcc
     7021 tacaaagaat cggttgattt ttttgtcatc aacaacaacc ggcagcttta caacggaaac
     7081 accacttggt acagctattt tactcaggta aagcgctatc agataatacc taaaggcaaa
     7141 aacaaactga aaatcatcca aagcggcaac atgttgagct gctacatcaa cgacgtcttt
     7201 gcctacgctt ccgaaacaga gaacacgcag ccgggctcag gcataggttt tatggtgcct
     7261 gccgggcaaa ccctttatgt ggatgacttc agactcagca agcgaactac agccgctgca
     7321 gcagccagaa ttaggaaatc gctgacagat aaactgaact ttacacctgt catgagcatt
     7381 attaacgaaa taaaaaacaa taaataaaac atcaccccat gccaaaattt gtgatttacc
     7441 aggttcttcc gcgtcttttc ggcaattaca acaccacccg aaaatacaaa ggaaccatcg
     7501 aagaaaacgg atgtggaaaa ttcaacagtt ttacaccccg ggcactggaa gaaatacgga
     7561 aattaggagc tacacatatc tggtacacag gcatcctgga gcatgccacc aaaaccgatt
     7621 actcggcttt cggcattcgg aaagatcatc ccggagtaat caagggcaac gccggatcac
     7681 cctacgccat caaggatttc tacgatgtag atcccgacct ggccgaaaac gtaacaaacc
     7741 ggatggaaga attcgaagca ctcatcaggc gaacgcacga ggcaggactg aaagcaatca
     7801 tcgactttgt acccaaccat acagcccgcc agtatcactc ggacatgaaa ccgaatggcg
     7861 tgaaggatat cggcgaaaac gacaatccgg actgggcatt ttctccgcta aacaattact
     7921 attacatacc caacgaacag ctccgcccga tgttcaacac cgaaggttat gatgaatatc
     7981 cggccaaagc taccggaaat gaccagttta ctgcctatcc gtcggaattt gattggtacg
     8041 aaaccgtaaa actcaactac ggagtcaact acatggaagg tcaccaaaaa caatttgacc
     8101 ccatacccga tacatggcat aaaatggcag acatactgtg ctactgggca gcaaaaaaca
     8161 tcgacggatt ccgcgtggat atggccgaaa tggtgcccgt agagttctgg ggctgggcaa
     8221 tacctcaggt gaaaaatata aatccggaaa ttattttcat tgccgaagtt tacaaaccgg
     8281 cagaatacaa caactatttg tgcaacggta aattcgatta cctgtatgac aaggttggcc
     8341 tctatgaaaa acttcgcggc attttgagcc ggaatgaagc agccagagat attacgggca
     8401 tctggcaaag cctaagccgc atctccgacc ggatgctcaa ctttctggaa aatcacgacg
     8461 aacagcgtat tgcttccgga tttttctgcg gcagcggtac gtacgccgaa ccggccatga
     8521 tcatttcggc aacactcacc aaagcacccg taatgatcta catggggcag gaactcggag
     8581 aactcgggat ggatatggaa ggtttcagcg gtgtggacgg caagacttcc attttcgatt
     8641 actggggagt ttcatccctg caagcctggg caaacaacgg tgaattcgac ggtgctaatc
     8701 tgactgacga acaatggaat atccgcaatt gctatcagaa actgctgaac atttgccttt
     8761 cagaaaaagc cattaccgaa ggcgaaatgt acgacctgga atttgccaac ttcggaaatc
     8821 ctgcattcaa tacgcatgaa cagtttgctt ttatccgaaa acaccggaac gaaattttac
     8881 tcatcgcagt gaatttctac gacaaaccgg tggaactgga gataaatata ccctacgaag
     8941 cattccagta tttgaaatta cacgacggcg attattacag cgtgcaaaat ctgctcaacg
     9001 aagaggaaga cctaccaata cagcggctca cctcatacat tccataccgc gtaaacttat
     9061 cagcatggaa cggaaaaatc atgaaactga aaaaagcaaa tgtggtatga gaagttagaa
     9121 gttgttaaaa ttaagttatt tattaaatcc ttttaacctt agactgcatt aaaacgacac
     9181 aactaaacac cggtctgatt tgttaaatat agtagccgga aaaacgagga atttttagta
     9241 cctttgtgcc cgcaatcaag aaaagacaat gtacaatata tacaatttat ttaatcaata
     9301 aacattatga gtaaaattga aaaaatcgta gcccgcgaag tactcgactc acgcggaaat
     9361 cccacagtag aagtagatgt aacactcgaa tcgggtgtag taggccgtgc ttccgttcca
     9421 tcgggtgcat ccaccggcga aaatgaagca atcgaacttc gcgacggcga caaaaaacgt
     9481 tacctgggta aaggtgtact gaaagccgtt gaaaatgtaa ataaagtgat tgctcctgcc
     9541 cttatcggtg aatgcgcact tcaacaacgc aaaatagaca aagcgatgat cgctctggac
     9601 ggaactaaaa caaaatcgaa cctcggtgca aacgccatcc tcggtgtatc actggctgtt
     9661 gccaaagctg ctgccaacta cctcgacatg ccactgtatc gttacattgg cggaacaaac
     9721 acatttacac tgccagttcc gatgatgaac attatcaacg gtggttcaca ctccgatgct
     9781 cctatcgctt tccaggaatt catgatccgt cctgtaggcg catgctgctt ccgcgaaggt
     9841 ctgcgtatgg gtgctgaagt tttccattca ctgaaaaaag tattgcacga taaaaacctg
     9901 agcactgctg taggtgacga aggtggtttt gctcctaatc tggcgggcgg tactgaagaa
     9961 gcactggaat caatcttgct ggctatcaaa aacgccggtt atgaacccgg taaagatgtt
    10021 atgatcggtc tggactgcgc ttcttccgaa ttctacaaag acggtatcta cgattatacc
    10081 aaattcgaag gcgctaacgc taaaaaacgt acttcagccg aacaggtgga ttacctggct
    10141 gaacttgttg ccaactaccc catcgactct atcgaagacg gtatgggcga aggcgactgg
    10201 gatggatgga aactgctcac tgacaaactg ggcaagaaaa tccagcttgt aggcgacgat
    10261 ttgttcgtta ccaacgtaga atacctgaaa aaaggtatcg acatgggttg cgccaactct
    10321 atccttatca aggttaacca gattggttcg ctcaccgaaa cactcgatgc tatcgaaatg
    10381 gctcaccgtg ccggttacac atcagtaact tcacaccgct cgggtgaaac cgaagatgca
    10441 actattgctg atatcgctgt agctaccaac tccggacaaa tcaaaacagg ttcattgagc
    10501 cgctccgacc gtatggccaa atacaaccag cttctccgca tcgaagaaga actgggcgac
    10561 ctggctgctt acggatggaa gaaataacaa cgaactatcc ctaaatccct ttctcttggg
    10621 aggggatctg aagaccggtt ttctgaaaag agaaccggtt ttttcgtttt tattatttag
    10681 cgtcccgaaa tttatctact ggttttcttt gaaattctgg attacgtatg gtttataaaa
    10741 gcctgtctta aaataaatat gacagatata gaagaaaata cgctcccaat caccttccga
    10801 taatccactc agataaattt cttttaaaat taagctgtaa ttaataaatt aaattatatc
    10861 tttgtttctc aaattttcaa aatccaatga acagaacagg agaaagaata aagaaaaaaa
    10921 gggaacagtt taatttacag ctaaacgaac tggctgaaaa aattggcata acttcaagcg
    10981 ccctttcgca aatcgaaaag ggaaaatcat ttccttccat actgacatta aagaatattg
    11041 ccgataaatt acacacaaca gttggtgaac ttatcggaga aaatgattca caggcaaaca
    11101 atccggtggt ttataaatcc gacattaaat ttatagacca gagcaagtcg ggaacaatgt
    11161 tctacctgtt atcgcaacac gatgtcaata agcagatgga tacatacctg gtaagatttt
    11221 ccaaggcttc cgctatcgat ggctttttct cagccgctta tggtcagata ttctcttatg
    11281 tgctcgcagg agaagtgcgg ttcgatctgg acggtaaaac ttttattctg aagtccggcg
    11341 acaatattta ctttaacgcc aaatcacagt tcaacgccat taattacttc gacggcgtta
    11401 gtgaaatcgt ctgggtacaa tctcctcctt cctattaatt caaaataaat taagtaatac
    11461 tttatttatt aagtaataat tattatcttt gcagtcgaaa caattagagc gatgacaaaa
    11521 tatcccgcgg ttttcttaga cagagacgga acattaatcg atgatgtggg ttacatcaga
    11581 cacacatcgg aagtaagttt ctacccgttt acatctgaag cgttactcat gctgcaggaa
    11641 cattttctgt tgttcgttat caccaaccag tccggcattg ccaaagggct taccacagaa
    11701 aaggaagtat ctgaagtaaa caaatacatt ttaaatacgt tgaagaagca ggatattatt
    11761 atacacgatc ttttttgttg cccgcataaa aacgaagata actgcgaatg taaaaaaccg
    11821 aaaacacagt ttattgagaa ggccgcaaca aaatacgata ttgatcttac gcgttcgttt
    11881 attatcggag atcacccgtc tgatgtacag tgcggaataa atgctggaat tacaccttta
    11941 tatttactga ctggtcatgg ccggaaacat aaaggcgaaa taagcaatga aacaattatt
    12001 tacacaaatc ttttagaagc ttctaagtat ataataaaca aaaacaagca gacatgaatg
    12061 atttattgac agttttaaga gaaaaaaata ttatcgttcc cgataatatt gctgagttgc
    12121 tcacgggatg caaaagttat aaggtattca ataccactga agaactttcc gaagcagcga
    12181 ctaacggaaa agagaatgag caatttgaag tgcaatacga cgttcccggt aaagggattt
    12241 acacagaggc aatcgtacaa cgtgtgaaga atggtatctc ggcaaattac accgagtcat
    12301 acatgcgccg cagagatccc gatacaatgg ttatcgcaga caataatcca acagacaaaa
    12361 tacgctttat caataaatat ggatatgaat tttcaggtct ccaaacagat acattcgaat
    12421 ggctgaaaca gcaggatctc gctgttttct tttacttcgc cggacgcgaa aatattggtt
    12481 cactgggtat tgccattgca cctgccaacg cggcgttttt tgccctggga ttgtcgatgc
    12541 tgcaggaaat ggttgcagtg gatgaattga aagataacgc ggaactaaaa tcggtgattt
    12601 ttgttgctcc tgtgttcaga catacccatt ttaatggtaa gcaggtagtt gttcacaacc
    12661 ggacagaaag catacacgaa ttatattcct acaatttata cccgggacca agtgcaaaga
    12721 aaggactata cggtgtgatg cttaaccagg cagaaaagga agactggatt attgctcact
    12781 gctcggccgt tcagtctgta agcccatatg acaaccttac cacctttatg cacgagggtg
    12841 caagtggagg aggaaagagc gaaatgcacc agcacattct ccgcgaaacg gacggacgtg
    12901 tgtcgttagg gcgtaattta ataacttccg aagaaaggtt tattaccatt ccccgctttt
    12961 gtactttcaa cgcagtagcc gacgatatgg ctttttgtca cccttcagta cagaaaaaag
    13021 atggtaaact ccgtatcatt gatgcagaaa atgcctggtt tgtgcgtgtc gacagtgtaa
    13081 agcaatatgg tgatgaccct tctttagaaa aaacgaccat aaatcccact tcgccgttgt
    13141 tgtttctgaa tattgaaact actcccggct ctacggcttt aatctgggaa catattcagg
    13201 ataaaccggg aaaatcatgt cccaatccaa gggtgattat tccccggaaa actgtgccaa
    13261 acgtagtcaa cagaccggtg tcggtcgata tcagaagttt tggcatccgc acacctgttt
    13321 gttcaaaaga aaatccttct tacggaatta tcggaatgtt ccatgttttg cctccggcat
    13381 tggcctggtt atggcgattg gttgcccctc gcggtcataa caatccaagt attgtcagct
    13441 cgggaggcat ggaaagtgaa ggcgttggtt cgtactggcc ttttgccact ggtcaaagag
    13501 taccacaggc aaatatgctg ctcaaacaga taattgatac accacgtact acttttacac
    13561 tggttcctaa tcaaaatatc ggcgtgtggc aggtaggttt caaacctcag ctgcttatgc
    13621 gcgagtacct gactcgaaga ggagctgccc aactgaaaac tgatcagtat caggctgccc
    13681 gttgtccact tttaggttac gaactcaatt atttgaccct tgaaggttca aaaatacctt
    13741 cgagattttt aaaggtttac aatcagcctg aagtcggctt tgagggctat gatgccggtg
    13801 ccgaaatgtt aagggcattt ttcaagtctg aattgcagaa atacctgaaa gaagacctgc
    13861 ttcaaacagg aaaacgcatt attgaagctt gtttgtcgga cgcaagtatt gaagattata
    13921 atgaaattgt tccaatgagt tatcagtacg aatattcctt caataaaata gaagactatg
    13981 agcaaagcag cgataaccct ggtttctaaa gtcttgcacg aatagactga ctaaacaagt
    14041 aacctattga aaatataaat tattgaattt caacataaaa atagaaatta ctgaaagacg
    14101 tctatagtat tttacaaaat gacaaataac aacattagtg aggcagcaca gataattaaa
    14161 gacggagggc tggtggcttt cccgacagaa acagtttacg ggttaggtgc aaatgcactt
    14221 aacccatttg ctgtcgctaa aattttcgaa gccaaggaac gcccttcatt cgatcccttg
    14281 attgtacata ttgcatcctt tgacgatttg aatatattat caaaaggaat aaacgagaat
    14341 gtaatccgtt tggcaaaaca tttctggccg ggcccattaa ccatcgtatt gccaaaaagt
    14401 tctgttgtcc cggatattgt aacttccggg cttaatactg tcggcattcg catgccggac
    14461 aacccgattg cacttgaact gataaaacaa gccggctgtc cgattgcagc accgagtgcc
    14521 aataagtttg gtttgctcag tccggtaaac gctaagcatg tgagaaagca acttcccggt
    14581 atcggttatg ttttggatgg aggtaacacc agggttggga ttgaatctac catcattgct
    14641 attgatggcc atacctgtca gctgttacgg ccgggaatta ttaccatcag cgatatagag
    14701 aaagcatttc cggatacttt tgtattcaat aattcgaaac cggataagct tgtctctccc
    14761 ggtttgttga aaagtcacta ttcgccccgt aaaccacttt actttctgga taacaggtta
    14821 gatttccccg agttttcagg cttgattttg cattgtgaga ccaatacctg ttataatgca
    14881 cagcgggtta tttatacatc acgaacacgc gataaaattg aaataaccgc caatctgttt
    14941 gcctcacttc atgcaatgga agaggacgac agcataaaaa acatttttat tgaacctgta
    15001 gaagaaacag gactgggtat agctatcatg gacaggttga aaaaagctgc ctatcgatac
    15061 aacgacagga tggattaaag gattaatgtg gactcacacc aaagaatcac aggataacat
    15121 aagcccaaag gaagcactat agcttataaa agaaggaaac ctctggtttg tcatcaatct
    15181 aaaaaatcat cagaaatcgc tgattttttg tccgtaaaaa accttataaa atatcaggct
    15241 tccaaaagat aatatccgga attctgaagc acaagaaagg gggaattcga atcccccttt
    15301 aattaaatta agataatgtg tctgtgatac ttttttttta ttcaatctct cttttgacag
    15361 tatgtatcat ttattcatgt tgagtttcca gactccatcc ttgttcacgt acgtactgtt
    15421 ctgagtatct tctgtaccgt taccgtaagt cacttttgtg gtaactgtcg ctgttttacc
    15481 atcttcagcg attttttctt ccaaaatttc aaaacttttc aagccacctt tagcatcggc
    15541 actttgtttg gttttttcag tgaatgcctt catcacctcg gctttctgtt cggccggagt
    15601 atcatcatcg ctttccacat tttcaaaaag catttctact gctttttcat agtttccttt
    15661 ttgtaactcg ttgtaaatgg ctttttcgat gtctttggga gattttccgg cgttgccgcc
    15721 actacaacct gcggtaaaca aaatcattgt cactgctgca aataaataca caattcgttt
    15781 catacttcat ttgtttttta aaagattgat aattattaaa aactgaaaac taattcaaac
    15841 tgaggattca aataaatatc cgacggaaaa atgtacattc cgccaaaaac aggcatatta
    15901 atccggcttc ttcttccgga actgatatcg acacctgcta aactgtaagc acaccacacc
    15961 agttcggtac aatacatttg cgtagtgtca ttcagcaggt aatcgtggtc gaataagatg
    16021 cccttgtcaa gcagtctgag cgcctgcgat gaagccatag cacctgtttt cgaatctttc
    16081 agacgataca cagcgccatg tactgccctt gcagtgctcc agaactcatc gagcgattcc
    16141 attttcactc tgtcaactgt atcacccttc tgccgctcac ccggagtgac gtgtatcaca
    16201 ccgaaacttt catcacgcct cacggctatg cctacgtggc tgtagattcc caacgaatcg
    16261 gcctgcatta cagcacgcga cttggctccg gtaccacggc gaaacaccaa atctccttcc
    16321 tccagtttta cagaagcaaa acggttagtt tctgctcttt cgcagcaaac tgtcagcaat
    16381 gaaagcagca ggagcatcaa catcactctc acgaccggca gcaacttcat ttttaatatc
    16441 attcgaagaa tggaattaag caaaatcaaa ttgtttgtaa aaaaaaaaca attcaactct
    16501 ttgactaata aatcataaca gatttttcac aaagtaaaat aaaaatcgtt aaacagcaat
    16561 aaattttaac aaaaaatccg taatcaaatc cggtaaataa aactgtaaat gcattaggcc
    16621 ctggataatt tgatagctca ctttttgaaa agtcaataca gagaatagtc gctatttcgc
    16681 ctgaataaga caattcacag aaaatcgctt ctttaattaa tctgaagaag agcaaaacaa
    16741 tctcatttac aaatcatttc catcagaaat aaccgatttt tttttgtatc tttgcagccg
    16801 aaaaaatttg tacccactga tccgactcgt gagaaccgga cagaataatt caaatgaatg
    16861 ttgaatcctc ctgaatacaa atcgggatta aacaaaaaac caatgggaaa tattgtagca
    16921 atagtaggac gacccaacgt aggcaagtcc acccttttta acaggctcac caaaagccgg
    16981 cgagccatag taaacgaaca ggcaggcact acccgcgacc gtcagtacgg taaaagcgaa
    17041 tgggaaggac gcgaattttc agtcatcgat accggcggat gggttgtcaa ttcttccgat
    17101 gtatttgaag aagagatcaa acgccatgtg atgcttgcca tcgaagaagc cgatgtcata
    17161 ctctttgtgg tcgatgtgca aaacggtctt accgacctgg acgaagctgt ggctcagata
    17221 cttcgccgct ctgaaaaacc aatcatgctg gtagccaaca aaacggacac atttgatctt
    17281 cagtatcagg ctgccgaatt ctacaagctg ggtttgggcg aaccttttat cctctcatca
    17341 atcaacggac tcggttcggg agaaatgctc gacgaactgc tgaaacactt caaatccgaa
    17401 gctaccgacg aaaccgaaga agaattgcca cgctttgcag tggtgggcag gcccaatgct
    17461 ggcaaatcat cgctcatcaa cgccctgatt ggcgaagatc gcaccatcgt caccgatata
    17521 gcaggtacta cgcgcgattc catttacatg cgcttcaaca aattcggaca tgatttctac
    17581 ctggtggaca ctgccggaat ccgcaaaaaa gccaaagtaa acgaagacct ggagtattat
    17641 tcggtggttc gttccatccg cgccatcgaa aactcggacg tatgcatcct gatgctcgat
    17701 gccacccgtg ggatagaaag tcaggacctg aatatcttct cgctgattca gaaaaataaa
    17761 aaaggactgg ttgtagttgt gaataagtgg gatttgattg aaaataagga agcaaacgac
    17821 acaaaaattt tcgaaaaaag catccgtgaa agacttgctc catttaccga ttttcctatc
    17881 attttcacct ctgtgccgaa caaacaacgt attttgaaag tacttgatac ggcagctaag
    17941 gtttatgaaa acaaaaaacg taaaattgcg acagcaaaac tgaacgaagt actcctgccg
    18001 attattgaaa aaagtccacc accggcactg aaaggaaagt atattaaaat taaatacatc
    18061 actcaacttc cgaacacgtt tatccctact tttgtgtttt ttgccaacct gcctcaatat
    18121 gtgaaagagc cttaccgcag gtttttagag aataaaatca gagacaaatg ggaacttcat
    18181 ggtacaccaa tcaacgtatt cattcgtcat aaatagaaaa aagcagaacc gcatggttct
    18241 gctttttttt atttcatcgt attattctta ctgttacttt ctaaaaaccg ctgtaaacta
    18301 cttccagttt catgggaata tctttattct tgccgctgaa aatggataca gcctgcattt
    18361 gtgtactttg attaacttcg gtgagcacag attgagagta acttgatgaa gatgcctgta
    18421 caagcgaaac cggtacgagc accatatcga ggtactcgtc ggtggagttc tttttcagct
    18481 cgttttcgat caggttattc aggctgctga atgtataatt gtatttatag gtagtagccg
    18541 taatgttttc ataacccagt gtggcgacaa acgatgcagt atccaccggc aattcatgat
    18601 tggtaaagaa tttatccaga tcacgttcgc ggataagcag caagcccgaa acataaggaa
    18661 tcagggcact tgtaccccat ttcgtgcgat cctgaacgtt aactttaagt atagcactgt
    18721 tgacatccag ctgtttgcca cccgttttta tccgctgacg gatgcgccct attggaatgt
    18781 ggactttggt atggtagtta gccggcgagg ctatgtaatt aaattcgtcg tcaggtcccg
    18841 atgaaagtgt tcggagtgga tgctgaacgc gatttacctt tacaatttct gcattggccg
    18901 gaaatgtatg ataactttta attttagcct gagcaggatc gtttttatat acatagtgat
    18961 agtaaagtgt cagtgaaaga ttattcacat tcaccatcac ggagctgcca aagtcagttg
    19021 taacatag
//
LOCUS       DAORKP010000027        18948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_150_length_18948_cov_10.373472, whole genome shotgun sequence.
ACCESSION   DAORKP010000027 DAORKP010000000
VERSION     DAORKP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18948
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_18948_cov_10.373472"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..221
                     /locus_tag="PLN75_02810"
     CDS             33..221
                     /locus_tag="PLN75_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03080.1"
                     /translation="MKKVFLFFAVASIFVACNKPAQKVEEAQDSIATEVVEAVDSTVA
                     VVDSAATAVVDSAATAVK"
     gene            323..1174
                     /gene="hisG"
                     /locus_tag="PLN75_02815"
     CDS             323..1174
                     /gene="hisG"
                     /locus_tag="PLN75_02815"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRG03081.1"
                     /translation="MLRIAIQTKGRLNEDTMSLLAETGIKLESAKRLLLMPARNFPVE
                     VLFLRDDDIPQSVADGVADAGIVGENEFVEKNKEAEIVKRLGFSKCRLSLAIPKENDY
                     TGIQWFEGKVIATSYPRILSEFLKSNQISAQIHVITGSVEIATGIGLADAIFDIVSSG
                     STLITNHLKEVETVMKSEAILIANKHLSEEKKAILDEFLFRAEAVQMAEDKKYVLMNV
                     PTENIEEIVQIIPGMRSPTIMPLAKAGWSSLHAVISEKGFWDIIGKLKNLGAEGILVV
                     PIEKMIL"
     gene            1343..2593
                     /locus_tag="PLN75_02820"
     CDS             1343..2593
                     /locus_tag="PLN75_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03082.1"
                     /translation="MHSSKILFAVILLISASQLAVSQNNTNSPYTRFGYGEISDGTAT
                     EIRGMGGVSLGNRSQNTINSVNPASYSSVDSLTFMFDLGAGMRYSHFSEKNQSKNTLN
                     ANLEYITMRFPLAKWLGFSAGLLPYSFAGYNFSQSDSLTIPRNSPAEDPYKVGYQQSF
                     SGSGGISQLYGGLSVALFNHISIGVNAYYLFGDASHYRALTFGTSSGYNSSLFTNQIK
                     ASDFKFRYGLQLYNTFADKHDVTLGLIYENKDKLNGEFTSTLNGDTIKNIKGFELPQT
                     LGVGLTYTFNKKLTVGADFTQQKWGDALFFGKTDSLVNTSKIAVGAEYIPNPSGRVRY
                     SDHIRYRIGFSTVNQYYRMGSSQPNNFTVSVGVGLPTRTGKSIMNASLEYGKIGSSTL
                     LREDYLKLTFSTSINEFWFFKPKL"
     gene            2597..3166
                     /gene="lptC"
                     /locus_tag="PLN75_02825"
     CDS             2597..3166
                     /gene="lptC"
                     /locus_tag="PLN75_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRG03083.1"
                     /translation="MRCSKKIAHYLTTALLVVVVLFEVSCSKEAPEKIDAVKDRKILP
                     ALSATKITTVISDSGITRYRIYTDKWDIYDRAAEPYWEFSKGIHFERFAENLTINATF
                     SSSYARYYERKSLWEFKGKVKAINLEGVMFETELLYWDESADRIYSDKFIKITRPNMI
                     ITGVGFEADQSLTKWKILNPKGPIYIKEN"
     gene            3303..3488
                     /locus_tag="PLN75_02830"
     CDS             3303..3488
                     /locus_tag="PLN75_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRG03084.1"
                     /translation="MLNNILLFSLGAPEIILIALVVLLIFGGKKIPELMRGLGKGVTS
                     FKKGLKDVDEEIKKDIE"
     gene            3522..4379
                     /gene="tatC"
                     /locus_tag="PLN75_02835"
     CDS             3522..4379
                     /gene="tatC"
                     /locus_tag="PLN75_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRG03085.1"
                     /translation="MKSTNKAISEKESTFWEHLEALRWTILRSLGVLLVLVFVGFGFK
                     DFIFNKIILPPLTSDFHTYRFLCYLAEVTKFPALCPEPFRIQMINITLSGQFMIHMLS
                     SLIIAVIIAVPYLLYEAWKFIKPALYPDEKRSVGMIFFSSSLLFYLGAAVSYFIIFPF
                     TLRFLGTYQVSEVIVNQISIQSYMNTLALLVFFMGLAFELPVVMYLLSQIGIVNKEML
                     RGFRKYAFVVVLILAAFITPTTDPFTMMIVALPIYMLYELSIVVCKKGTDTPADSEAE
                     NDTDTNATE"
     gene            4563..5075
                     /locus_tag="PLN75_02840"
     CDS             4563..5075
                     /locus_tag="PLN75_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRG03086.1"
                     /translation="MSTSSVKLYSLEFTNVKTYLFAVVFVVGNLLLPQLAHLIPQGGL
                     IFLPIYFFTLIAAYKYGIRVGLLTAVFSPVVNNLLFGMPPAAVLPAILIKSILLAIAA
                     AMAARHFGKVTFIGVLLAVLAYQFIGMGAELAITQSFSAAVQDFQIGIPGIILQLTAG
                     YLALKALEKV"
     gene            5076..5474
                     /locus_tag="PLN75_02845"
     CDS             5076..5474
                     /locus_tag="PLN75_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRG03087.1"
                     /translation="MEQKSFNEVLQRFREIEFHENFDMIVAIANGGIIPAAILNQRLQ
                     TDFQILKINLRDINQKPKYENPQLLAPVGFDFHNKTILLVEDRIKTGATVNFAIGLLQ
                     GAAQIKTFAVNGNADYALYNESCFRFPWNI"
     gene            5502..6476
                     /locus_tag="PLN75_02850"
     CDS             5502..6476
                     /locus_tag="PLN75_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRG03088.1"
                     /translation="MDRRKFLKSVAITGAVAVGGKTLFKESAGMDILTQTFAGSASAA
                     TVSGVNAKYDLVAVLGGEPEVMFRKAIAEMGGMGNFIKKGDKVVVKPNIGWDKTPDLA
                     ANTNPKLVEEIIRQCFKAGAKEVVVFDHTCDDWRKCYKNSGIEAAAKAAGAKVVPADQ
                     GSYYKKVSLPNGKKLTQTMIHESIVNSDKWINVPVLKNHGGAQLTISMKNYMGIVWDR
                     GFFHANDLQQCIADVCTYEKRPVLNVVDAYRMMKTSGPRGKNESDVVLTKGLFLSQDI
                     VAVDTAAANFFNQVREMPLSKVSHLAKAQELKVGTMNLDALKIKRIKL"
     gene            6500..8026
                     /locus_tag="PLN75_02855"
     CDS             6500..8026
                     /locus_tag="PLN75_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRG03089.1"
                     /translation="MLKKIRIGFSIVLLVLITLFFLDFAGILPLGFHSVTKIQLVPAL
                     LALNINVLIILLLLSVVFGRVYCSFICPMGVYQDVVAWISKKFTKKKKYTYSKAKTIL
                     RWTIFGASVISFIFGFNFLVGLLDPYGAYGRIVTHIFRPAYLAGNNLLEYIFSSAGNH
                     TFYKVGIYGLGLFSTLIALATLIGIGLLAWRNGRTYCNTICPVGTLLGFISRYSIFRI
                     QFDDSKCNSCGLCTMNCKASCINSKDKEVDYSRCVTCFNCVEACNRNAMHYAPYKRSK
                     KTSIQQPQENIDESKRRFLSATIITATAATGLLAQKVALPLDGKRSIKRNLPIVPPGG
                     LRVDNLQEKCISCHLCVSKCPSHVIKPAFMEYGLGGMMQPRLYFEHGFCNYDCTVCTD
                     VCPSGALLPITKEEKHRSQVGQVRFVRDNCIVYTDETNCGACSEHCPTQAVHMVPYKN
                     ELTIPETDVSICVGCGGCEYVCPAIPHKAIYVEGLKKHNILEFKEEKPEEVEVNDFGF
                     "
     gene            8408..9934
                     /locus_tag="PLN75_02860"
     CDS             8408..9934
                     /locus_tag="PLN75_02860"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HRG03090.1"
                     /translation="MITSTQIGFDNEKYLQEQSEAILDRVSRFNDKLYLEMGGKIIYD
                     YHAARVLPGFDPNVKIRLLQKLKDKIDVILCVHAGDLERNKVRADFGITYEVDAFKSI
                     DDLKWFGIHVAAVVITRFKDQPAAINFKNKLELKGIKVYLHYPTKGYPTDVDKIVSDE
                     GYGANEYIQTSKPIVVVTGPGPGSGKLATCLCNLYHEYKSGVKAGYAKFETFPIWNLP
                     LNHMVNVAYEAATADLRDKNMIDTHHLKAYGIETVNYNRDIDAFNLLKRILEKITGGE
                     AMYQSPTDMGVNRAGAGIVDDKVVQEAAYQEVIRRYFRSAVEYAMGLTGKSTLDRSLD
                     IMNRIGAKESDRKVVAAARNAAAEAEEKKKGYGGIFSGAAIELHDGTIITGKSSPLFH
                     SSSSMILNATKHLAGLPDSMLLLPESIIDSVTHLKKDILNGKQVSLDVEETLIALAIS
                     ATSNPAAKMALDKLTELYGCEMHSTHIPTPGDEAGCRKLKVNLTCDPVFSSKRLFISE
                     "
     gene            complement(10011..10298)
                     /locus_tag="PLN75_02865"
     CDS             complement(10011..10298)
                     /locus_tag="PLN75_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03091.1"
                     /translation="MEEHTDYVEFDRPDGKKVRIVFELYRYEILFHYCEFRHDENSSW
                     QSLSKGDDGYFAYNVAEADGVEKTEKVKFPTPEEIMTAQDEINKRYNILSI"
     gene            complement(10350..11801)
                     /gene="mnmE"
                     /locus_tag="PLN75_02870"
     CDS             complement(10350..11801)
                     /gene="mnmE"
                     /locus_tag="PLN75_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRG03092.1"
                     /translation="MNTKNDNQLSSNSPIQGNREHEKTICAISTPPGSGGIAVIRVSG
                     YEAPGICDKIFRFGKDNEKLADKSGYSVHYGYITDKEKQEVDEVLVTVFRAPHSFTGE
                     DVVEISCHGSVFIQQKILRLLIQKGCSLAKPGEFSMRAFRNGKMDLSQAEAIADLIAS
                     TNEASHRMAMNQIRGGFSAELKNLREKLLHFVSLVELELDFSEEEVEFADRTQLAALA
                     GEIHERIRSLTDSFKIGNVLKTGIPVALVGETNVGKSTLLNLLLNEERAIVSDIHGTT
                     RDSIEDLVNIEGITFRFIDTAGIRKTTDEIEKIGINLTYKKIEQSDIVLWIVDCTQVT
                     EHIEWLTEKIAKRAQGKKIILVFNKIDKLADDEREVLDDLFKQFEGERIYISAKKRIN
                     TDNLTKALIHASQLERIKPDDVIVNNIRHYEALLLAQQDIQRVIEGIENRISGDFLSQ
                     DIRECMYHLGEITGQISNDEILASIFSKFCIGK"
     gene            complement(11914..12786)
                     /locus_tag="PLN75_02875"
     CDS             complement(11914..12786)
                     /locus_tag="PLN75_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRG03093.1"
                     /translation="MMKHFPPSELIINDDGSIFHLHLKPEQISDKIILVGDPGRVALV
                     ASYFDEQECSVSSREFNTITGTYKGKRLTVVSTGIGTDNIDIVVNELDALANIDLETR
                     TEKPFFRQLNLVRIGTSGGMQPEIPLGSFLISEKSIGFDGVLNFYAGRDGVCDLEFEE
                     EVKKQLKWNPHFCAPYVVDADAELVNKIGQEDMLRGVTISANGFYGPQGRVLRIDLAE
                     PELNERIENFSFGKNKITNYEMEGSAIAGLARLMGHKAMTVCCIIANRRVEAANTDYK
                     PYIEKLVLTVLDRI"
     gene            complement(12983..13150)
                     /locus_tag="PLN75_02880"
     CDS             complement(12983..13150)
                     /locus_tag="PLN75_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRG03094.1"
                     /translation="MAEKKENRTKNFVLRMDHETMEAIEKWAAEEFRSTNGQIQYLLE
                     QALIKSGRKKR"
     gene            complement(13165..14136)
                     /locus_tag="PLN75_02885"
     CDS             complement(13165..14136)
                     /locus_tag="PLN75_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRG03095.1"
                     /translation="METQEKKFSGIKLPGIPMIILNLLLLIAIVLLFVWAVSSQTLSS
                     GAITFYAILSTVLFIINTIFWGGFMQIEPNEARVMVFFGEYRGTVKDNGFFWVNPFYA
                     KKKLTLRARNLDAEPIKVNDKTGNPIMIGMVVVWKVVDTYKASFEIDNASMAPSIPGT
                     GSYDNVNTKMKAYENFVQIQSDAALRFVAGMYPYDLLNTESENQLTLRSGGEEMNEIL
                     EAQINERLKIAGIEVVEARINYIAYAPEIASVMLRRQQADAIIAAREKIVEGAVGMVK
                     MALDKLSADGIIELDDEKKAAMVSNLLVVLCGESNAQPVINSGTLYN"
     gene            14287..15291
                     /locus_tag="PLN75_02890"
     CDS             14287..15291
                     /locus_tag="PLN75_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG03096.1"
                     /translation="MRIVEKVIWGVIGAGNVCEVKSVPGMYKTPFSEVKMVMRRNAEK
                     AADFARRHGIPNSTTDADVIFNDPDINIIYVGTPPDTHCEYTLRAAAAGKAVYVEKPM
                     ANNADECKKMIDACMRAEVPLFVAYYRRALPGFNRLGELIHSGKIGEVRFVEIEMWRA
                     PRSYDLNTDGNWRVFPEISGGGHFHDLASHQLDYLDSLFGLVTQAKGIGVNQAGLYPA
                     DDLVAGTFVFENGVCGSGMWCFTADKSSEKETITILGSKGHLHFNTFGSPLEIHITTS
                     EGSELIEIEHPDHIQQPLIQTVVNELRGIGTCPSTGITAARTTGVMEKITSRIELTVR
                     "
     gene            complement(15384..15455)
                     /locus_tag="PLN75_02895"
     tRNA            complement(15384..15455)
                     /locus_tag="PLN75_02895"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15421..15423),aa:Met,seq:cat)
     gene            15721..18828
                     /gene="secDF"
                     /locus_tag="PLN75_02900"
     CDS             15721..18828
                     /gene="secDF"
                     /locus_tag="PLN75_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRG03097.1"
                     /translation="MQNKGLVRILGIALALICLFYLSFNLVTMRFENKAELASTTAKE
                     TFQKSNTFASLTAQDKSIKLDSIGNRAYYSYLDSVGKQKVYLNYTLKECREKGVNLGL
                     DLKGGMNVTLEISVPDIIRALSNYNTTPNFTKALQVATERQKSRSGTSYLDLFVQAYN
                     EIDPQAKLATIFSTFELKDQISLSSTNEQVIAVLKGEIESAINNSFNVLRTRIDRFGV
                     IQPNIQKLENTGRILVELPGVKEPERVRKLLQGSANLEFWATYNLNEIINNLVEANKV
                     IAQLNIAGPTIDSTSVTAPDTSKVVAQTSDKTATSSIDSLKAALTTKSQSAADSLKMM
                     EQNKKENPLFAILAVNVQNGQVIPGPQVGVVEGKDTALVNTYLKMKQVKEVLPRDLAF
                     RWGVNAIDENDKYYQLIAIKQSTRDGRAPLTGSVITDARDQFGQHSSFAEVSMTMNAE
                     GAKTWARMTKENIGKSIAIALDGYIYSYPTVQNEITGGQSSITGRFTPEEAKDLANVL
                     KSGKMPAPARIVQEDVVGPSLGEEAINSGMWSFLVAFLLVMLYMILYYGLIPGIIADI
                     ALFINVFFLMGILAAFGAVLTLPGIAGIVLTLGMAVDGNVLIYERVREEIESGKNMKK
                     AIHDGFNNAISAIIDANVTTLITGIVLFIFGQGPIQGFATTLIIGIVTSFVTSVFLTR
                     MMLEMYANRENAKQLPFTTGATKTWFQNLSIDFIGKRRIAYMISGALILVSILGFGIR
                     GMSYGIDFSGGRNYIVQFDQKIQTEEVRKLLESTFDGASTQVISIGTNNDKVRISTKY
                     KIDVTSETIDDEISAKLYEGLKPMLGNTSLADFTQKNIVNSQKVGPTIADDIKTSSVW
                     AVIVALLLVSVYIFIRFNDFGFSMGAMASLLHDVIVIIGVFALLWGIMPFSMEIDQSF
                     IAAILTYVGFSVNDTVVIFDRIRENIRLYPKRDRKEIINDALNDTLSRTFSTSMSVFV
                     VLLAIFLFGGDTIRGFVFALLVGTILGVYSTLFVAVPIAFDIIEVRKKKKASKELTAV
                     PVTKK"
BASE COUNT         5509 a         3845 c         3971 g         5623 t
ORIGIN      
        1 aacatgttaa atttttaaag gattaaaaca aaatgaaaaa agtattttta tttttcgctg
       61 tagcttcaat attcgtagct tgcaacaaac cagctcaaaa agttgaagaa gcacaagatt
      121 caattgcaac tgaagttgta gaagctgttg attctactgt tgctgtagtt gactctgctg
      181 ctactgctgt tgttgactct gctgctactg cagttaagta attaagcaca gccgacttga
      241 agtcacaaaa gcccgttgaa aaacgggctt ttattttttc ggcaaattat gtatttttgc
      301 aacctataac ccaacataca ttatgctaag aatagctata caaacaaaag gaagactcaa
      361 cgaagatacc atgtcgctgc ttgccgaaac aggtataaag cttgaatcag ctaaacggtt
      421 gctccttatg cccgccagga attttccggt ggaggtgctt tttctcagag acgatgacat
      481 tccacaatcg gtggccgatg gagtagccga tgcgggtatt gtgggagaaa atgaatttgt
      541 tgaaaaaaac aaggaagcag aaattgttaa acgactgggt ttcagtaaat gcagactctc
      601 gctggcaata ccaaaagaga atgattatac tggtattcaa tggtttgaag gaaaagtaat
      661 cgccacatca tatccacgaa tacttagtga atttctcaaa tccaaccaaa tcagtgcgca
      721 gattcatgtt attaccggtt cggttgaaat agcaacagga attggacttg cagatgcaat
      781 ctttgatatt gtaagttcag gcagtacgct gataaccaat catttgaagg aagttgagac
      841 tgtgatgaaa tctgaagcta tactcatagc taacaagcac ttatctgaag agaaaaaagc
      901 catactggat gaatttttgt ttcgtgcaga agcggtacaa atggcagaag ataaaaaata
      961 tgtactgatg aatgtgccta ccgaaaatat cgaagagatt gtacaaatca ttccgggtat
     1021 gagaagcccc accataatgc cattggctaa agcagggtgg agttcgctgc atgcagtcat
     1081 tagcgaaaaa ggattttggg atattattgg caaattaaaa aatcttggtg ctgaaggaat
     1141 cctggtagtg ccgatcgaaa aaatgatttt ataaacctct gaaactctta ttttacggat
     1201 aatttaaact tccagagacg tggtttcagt ttttttaacg gcatgagagt ttatttattt
     1261 tcacaagaaa ttaaaatgtg ccatctttgc atattcaaaa atttaaagtt aaaaaaacgg
     1321 attcttttcc acaatacatt aaatgcacag ctcaaaaata ttatttgcag taattcttct
     1381 tatctcagct tcacaactcg ctgtaagtca gaataacacc aattcgcctt atacgcgttt
     1441 cgggtatggt gaaatttctg atggaacagc cactgaaatt cgcggaatgg gaggcgtttc
     1501 gctcggaaac cgctctcaaa atacgatcaa cagcgttaat cctgcttcat attccagtgt
     1561 ggatagtctt acatttatgt ttgacctggg agcaggaatg cgctattccc atttttctga
     1621 aaagaatcaa tccaaaaata cgctgaatgc aaacctggaa tacattacca tgcggtttcc
     1681 gcttgctaaa tggcttggtt tcagcgcagg acttctgcct tattcgttcg caggatacaa
     1741 cttcagtcag tctgattcac tcactatacc ccgaaattct ccggcagaag atccatacaa
     1801 agttggttat cagcagtcat tttcaggctc aggaggcatt tcgcaactgt atggaggatt
     1861 gtcggtagct ctcttcaatc atatttcaat cggcgtaaat gcatattatc tttttggaga
     1921 tgccagccat taccgcgcac tcacttttgg tacttcgtcg ggttataatt cctcgctttt
     1981 caccaatcag atcaaagctt cagatttcaa attccgatac ggattacaac tttacaatac
     2041 atttgccgat aagcatgacg taacgctcgg tttgatttat gaaaataaag acaaactgaa
     2101 cggtgaattc acctctacac tcaacggtga cacgattaaa aacataaaag gttttgaact
     2161 gcctcagact ctgggagtag gtcttaccta tactttcaat aaaaaactca ccgtcggagc
     2221 tgattttacg cagcagaaat ggggtgatgc tttattcttc ggaaaaaccg attcgctggt
     2281 taatacttca aagattgctg tgggagctga atatattccg aatccatccg gacgcgtcag
     2341 gtattcggat catatccggt atcggattgg attcagcaca gtgaatcaat actacagaat
     2401 gggaagttcg cagccgaata actttacagt aagtgtcgga gtaggattgc ctaccagaac
     2461 cggtaaaagt atcatgaatg catcgctgga atatggcaaa attggctctt cgaccctgct
     2521 acgcgaagat tacctcaaac ttacattcag tacttctatc aacgaattct ggttcttcaa
     2581 gccaaaactg taatgcatga gatgttcgaa aaaaatagct cactatctga caaccgccct
     2641 tttggtggtt gttgtgttgt ttgaagtttc gtgttcgaaa gaagctcccg agaaaatcga
     2701 tgcggtgaaa gaccgcaaaa ttcttcccgc ccttagcgca accaagatta cgaccgttat
     2761 ttcagattcc ggcatcaccc gctaccggat ttacaccgat aaatgggata tatacgaccg
     2821 cgctgccgag ccatactggg aattttccaa aggaattcat tttgagcgat tcgccgaaaa
     2881 tctcactata aatgctacat tcagcagtag ttacgcccgt tattatgaaa gaaaaagtct
     2941 gtgggaattc aaaggtaaag tgaaagcaat caatctggaa ggagtcatgt ttgaaactga
     3001 acttttatac tgggacgaat ctgccgacag gatttactca gacaagttta ttaagatcac
     3061 acgaccaaac atgataatta ccggcgtggg ttttgaagcc gaccaatcgc tcacaaaatg
     3121 gaaaatactg aatccaaaag gtcctattta tatcaaagaa aattgaaaaa acatcacatt
     3181 ttcagccgtt tacaaagtta tatcagatga taaacaaaga ttttttgttt tattcaaata
     3241 aaaacctgaa aataatctta tatttgtaat tcaatcaacc aacaattaat acataaaaac
     3301 gaatgcttaa caacatctta ttattttcac tcggtgctcc tgaaattatt ctgattgcgt
     3361 tggtagtgct tttaatcttt ggtggtaaaa aaattcctga actgatgcgc gggctcggca
     3421 aaggtgttac aagtttcaaa aagggtttga aagacgtcga cgaagaaatt aaaaaagaca
     3481 tcgaataatt cctttcatct gttttctgta tcgggagaaa gatgaaatca acgaacaagg
     3541 caatttctga aaaggaaagc acgttttggg aacatttgga ggcattgcgt tggacaatac
     3601 tccgaagcct gggtgtgctg ttggtgcttg tgtttgttgg atttgggttt aaagacttca
     3661 ttttcaataa gataattctc cctcctctta cctctgactt tcatacctat cgttttcttt
     3721 gttatcttgc cgaagtcacc aaatttccgg cattatgccc cgaacctttc cggatacaga
     3781 tgatcaatat cacgctttca gggcagttta tgattcacat gctttcttct ctgattattg
     3841 cagtgattat tgcagtgccc tatctgctgt acgaagcctg gaaatttatc aaaccggcgc
     3901 tttatcccga tgaaaaaaga agtgtaggga tgatcttttt ttcttcttca ttgctgtttt
     3961 atctgggcgc tgcagtaagt tatttcatta ttttcccttt tacgctacgt tttctgggca
     4021 cctatcaggt atccgaagta attgtaaatc agatttcaat ccaatcgtat atgaatacgc
     4081 tggctttgct ggtgtttttt atggggttgg catttgagct gcctgtggta atgtatctgc
     4141 tttctcagat tggcattgtt aataaagaga tgcttcgtgg tttcagaaaa tatgcttttg
     4201 tagttgtgct tattctggca gcattcatca cacctaccac cgatcctttc accatgatga
     4261 tagtagcatt acctatctat atgctctatg aactgagcat tgtagtatgc aaaaaaggaa
     4321 cagacacacc agccgactct gaagcagaaa atgacactga cacaaatgca actgaataag
     4381 tgttaatttc acccgccgtt ttcacgaaaa aatcaaaagt cttttcttat tgcctgaatt
     4441 tcaacatttt ttaatataaa atgaatctat cgataggcac aaattgcgaa aaataaccta
     4501 tttttgtaaa attgcatcgt tatggtcaat tatcagagat aaataattta atcagaaaaa
     4561 atatgtcaac atcatcagta aaactttatt cattggaatt caccaatgtc aaaacgtatt
     4621 tgtttgcggt agtattcgta gttggaaatt tgctgctacc acagctggca catctcattc
     4681 cgcagggtgg attaatcttt ttgccaattt atttctttac actcattgct gcttataagt
     4741 acggcattcg ggtaggattg cttacagcgg tattttctcc ggtggtaaac aatttgctat
     4801 tcggtatgcc accggcagct gtactgcctg ccatactcat caaatcaata ttgcttgcta
     4861 tagctgcagc catggcagca aggcattttg gcaaagtaac atttatcgga gtactgttag
     4921 ctgtgctggc ttatcagttt attggtatgg gagccgagtt ggcaataacg cagagttttt
     4981 ctgcagctgt tcaggatttt caaatcggca ttccgggaat tatactacag cttacagccg
     5041 gatatttagc tttaaaagca ttggaaaagg tgtaaatgga gcaaaaatca tttaacgaag
     5101 tcttgcagcg ctttcgtgaa atagaatttc atgagaattt tgatatgatt gtggctatag
     5161 ccaatggtgg aattattcct gccgccatac tcaatcagcg tttacagact gattttcaga
     5221 tattaaagat taatctgcgg gatataaatc aaaaaccgaa atatgagaat ccgcagttgc
     5281 tggctccggt cggttttgat tttcacaata aaactattct cctggttgag gatagaataa
     5341 aaacgggtgc aactgttaat tttgccatcg ggctgctgca gggagccgcg cagattaaaa
     5401 cttttgcagt caacggaaat gccgattatg cactctacaa tgaatcatgt tttcggtttc
     5461 cctggaatat ttagcgtttt atctttacaa aaaaaaacac aatggacaga agaaaatttt
     5521 tgaaatcagt agccattaca ggtgctgtgg cagtaggagg aaaaacactt ttcaaggaaa
     5581 gtgccggaat ggatatactc acacagacat ttgccggcag tgcaagtgca gcaacagttt
     5641 cgggtgtgaa tgcaaaatac gatttggtag ctgtgctcgg aggcgaaccc gaagtgatgt
     5701 ttcgaaaagc cattgcagaa atgggtggaa tgggtaattt cataaaaaaa ggagataaag
     5761 tggtggtgaa acccaacatc ggatgggata aaacacccga tctggcagcc aatacaaacc
     5821 caaaactcgt ggaagaaatt atcagacaat gtttcaaagc cggcgccaaa gaggtagtcg
     5881 tatttgatca tacgtgcgac gactggcgta aatgctataa aaacagcggt attgaagcag
     5941 cagccaaagc agccggagct aaagtggtgc ccgccgatca gggatcgtat tacaaaaagg
     6001 tatcgcttcc taacgggaaa aaactgacac agactatgat tcatgaatcg atagttaatt
     6061 ctgataagtg gattaatgta cctgttctga aaaatcatgg tggagctcag cttaccattt
     6121 ccatgaaaaa ctacatgggt atcgtgtggg atagaggttt tttccatgcc aacgatctgc
     6181 agcaatgcat agctgatgtg tgtacatacg aaaaacgacc tgtactcaat gtagtggatg
     6241 cctaccgcat gatgaaaaca agcggtccgc gaggtaaaaa cgaatcggat gtagtgctca
     6301 ccaaaggttt gtttctgtcg caggatattg tagctgtgga tactgcagcg gccaatttct
     6361 tcaatcaggt acgtgagatg ccactctcaa aggtaagcca tctggctaaa gcgcaggaac
     6421 tcaaagtggg aaccatgaat ctcgatgccc tgaaaattaa aagaatcaaa ttataatttc
     6481 aattaagcac aaccctttta tgttgaaaaa aatcagaatt ggattttcaa tcgtgctttt
     6541 ggtgttgatt acactgtttt ttcttgattt tgcaggtatt cttccattag gttttcattc
     6601 ggttaccaaa atacaactgg tgccagctct gctggcacta aatattaatg tgctcatcat
     6661 tctgcttctg ctttcagttg tatttggacg cgtgtattgc tcatttattt gcccaatggg
     6721 cgtatatcag gatgtagtgg cgtggatatc aaaaaagttt accaaaaaga aaaaatacac
     6781 ctatagcaaa gccaaaacga tacttcgatg gactatcttc ggtgcttctg tgatttcatt
     6841 tatctttggt ttcaattttc tggtcggatt acttgacccc tacggtgcat acggacgtat
     6901 tgtaactcat atttttcgac cagcctattt agccgggaat aatctgctcg aatacatatt
     6961 ttcaagtgcc gggaatcata ctttttacaa agtcggtatt tatggacttg gacttttttc
     7021 cacactaata gcactggcta cgctcatcgg tataggtctg ctcgcctggc gcaatggtcg
     7081 tacatactgc aatacgattt gccctgtagg tacgctgctc ggatttatca gccggtattc
     7141 cattttccgg attcagtttg atgatagtaa gtgcaacagc tgcggtcttt gcaccatgaa
     7201 ctgtaaagcc tcctgcatca actccaaaga taaagaggtg gattacagca ggtgtgtgac
     7261 ctgttttaat tgtgttgaag cttgcaatcg gaatgccatg cactatgctc cctacaaacg
     7321 gtcgaaaaaa accagcattc aacagcctca ggagaatatc gacgaatcga aacgtcgttt
     7381 cctgtcggct acaattatta cagctactgc agcaacaggt ttgcttgctc agaaagttgc
     7441 actgcctctc gatggtaagc gcagtattaa acgcaacctg cccattgttc ctcccggagg
     7501 acttcgtgtt gacaatctgc aggaaaaatg catttcatgt catctgtgtg taagtaaatg
     7561 tccttcgcat gtcatcaaac ctgcctttat ggagtatgga ctgggtggga tgatgcagcc
     7621 ccggctatat tttgaacacg gattttgtaa ctacgactgt acggtttgca ccgatgtgtg
     7681 tccctccgga gcgcttctgc ccattacaaa agaagaaaaa caccgctcgc aggtagggca
     7741 ggtgcgattt gtgcgcgaca attgcatagt ctataccgac gaaaccaatt gcggagcctg
     7801 ttctgagcat tgccccacac aggcagtaca catggtgccc tacaaaaatg aactcaccat
     7861 tcccgaaaca gatgtaagta tctgtgtagg ctgtggcgga tgcgaatacg tttgtccggc
     7921 cattccacac aaggctattt atgtggaagg cttaaaaaag cacaacatac tggaatttaa
     7981 agaagaaaaa cctgaagagg tggaagtaaa tgatttcgga ttctgatcag aataattgaa
     8041 tatttaatat caatctgaat ttccaggtag aaaattgaat tagaataaaa gatttttttc
     8101 aaaaaaacaa ggaggcatca cattgaaagt atgcctcttt tttttggaaa aaatgcagga
     8161 agaatgattt attgaaatgc ggttgtaaat ctattgttgc ctgaattgta tttttgtaaa
     8221 atataccaga tgactgaaaa tttatttttt caactatctc aagcatgtga tagtaaatgt
     8281 tttaatggtc ttcattttgt gtctgcagat ttatttttgt tttttagtca tataaagttg
     8341 atgcaatttc agaaaaaatg agtaattttg aatcccgata caaacactcc taaaactaca
     8401 tcgggacatg attacttcaa ctcaaatcgg ttttgacaac gagaaatatc ttcaggagca
     8461 atctgaagct atacttgaca gggtaagcag attcaacgac aagctttatc ttgagatggg
     8521 cggcaagata atctatgatt accatgcagc acgtgttttg cccggatttg atccaaacgt
     8581 aaaaatccgg ttgcttcaga agctgaaaga caagattgat gtcattctgt gtgtacacgc
     8641 cggcgacctc gaacgaaaca aagttagagc cgatttcgga attacctacg aagtggatgc
     8701 attcaaaagt atcgacgacc tgaaatggtt cggaattcat gttgctgctg ttgttattac
     8761 ccgcttcaaa gaccagcctg cagccattaa tttcaaaaac aaacttgaac tgaagggtat
     8821 caaagtgtat cttcattacc ccaccaaagg ctatccgacc gacgttgata aaatagtgag
     8881 cgatgaagga tatggagcca atgaatatat acagacgtcc aaacccattg tggtggttac
     8941 cggacccgga cccggcagcg gaaaactggc tacctgcctc tgcaatctgt atcacgagta
     9001 caaaagtgga gtaaaagccg gatatgccaa gttcgaaact tttccaatct ggaacctgcc
     9061 tctcaatcac atggtaaatg tagcctacga ggctgctaca gccgatcttc gggataagaa
     9121 tatgatagat actcaccacc tgaaagcgta tggtattgaa accgtgaatt acaaccgcga
     9181 tatcgatgct ttcaatctcc tgaaacgaat tcttgagaag attaccggag gcgaagctat
     9241 gtatcaatcg ccaaccgata tgggagtaaa ccgcgccgga gccggaattg ttgacgataa
     9301 agtggttcag gaagctgctt atcaggaagt tattcgcagg tatttcagga gcgctgtgga
     9361 atatgccatg ggattgacag gaaagtcaac gctcgaccgt tcgctggata taatgaaccg
     9421 aattggtgcc aaagaaagcg accgtaaagt agtggctgcc gcccgaaatg ccgccgcaga
     9481 agcagaagaa aagaaaaaag gatatggtgg aattttcagt ggtgcagcta ttgaattgca
     9541 cgacggaacg attattaccg ggaaaagctc tccgctcttt cactcctcct ccagtatgat
     9601 actgaatgca accaaacatc tggcaggatt gcccgacagc atgctcctgc tgcccgaaag
     9661 catcatcgac tcagtcacac acctcaaaaa agatattttg aacggtaagc aggtaagcct
     9721 ggatgtggag gaaaccctca ttgcccttgc aataagtgct acctccaatc cggctgctaa
     9781 aatggctttg gataaactga ccgagctcta cggttgtgaa atgcactcca cgcatatacc
     9841 tacaccggga gatgaagccg gatgccggaa attgaaagta aacctgacct gtgatcctgt
     9901 tttttcgagc aaaagattat ttatcagcga ataaaattct gatattaaat gaatgtgatt
     9961 tttaaatcaa taaaaaaagg tctcacctga acgagacctt ttccatttta ttaaatgctt
    10021 agaatattgt atcttttgtt aatttcgtcc tgagcagtca taatttcttc cggtgtcggg
    10081 aacttcactt tttcagtttt ttcaacacca tcagcttcgg caacgttata agcaaaatag
    10141 ccgtcatcac cttttgaaag gctttgccag ctgctgtttt catcgtgtct gaactcgcag
    10201 tagtgaaaca gtatttcgta gcggtaaagt tcaaaaacta ttctgacttt cttcccgtcc
    10261 gggcggtcga attcgacata atcagtatgt tcttccattt gatttgaatt aacttagttt
    10321 tgctgctatt tttttaaaaa tataatgaat tatttgccta tacaaaattt cgaaaatatg
    10381 gatgcaagta tctcatcgtt agatatttgc cccgttattt cacccagatg atacatgcat
    10441 tcgcggatat cctggctcag aaaatcgccc gaaattctgt tttcgatgcc ttcaatcacc
    10501 cgttggatat cctgctgggc cagcagaagg gcttcataat gacgtatgtt gtttactatt
    10561 acatcatcgg gttttatccg ttcgagttgc gaggcatgta taagtgcttt cgtcagattg
    10621 tctgtattga ttcttttttt tgcagaaatg taaatccgct caccttcaaa ctgtttgaac
    10681 agatcgtcaa gtacttcgcg ctcgtcgtca gccagtttgt ctatcttgtt gaataccaat
    10741 attattttct ttccctgtgc tcgtttggct attttttcgg tcagccattc gatgtgctcg
    10801 gtcacctggg tacaatccac tatccacaat acgatgtcgg attgctcaat ttttttgtac
    10861 gtcagattga tcccgatttt ttcaatttca tctgttgttt tgcggattcc ggctgtatca
    10921 atgaagcgaa atgtaatgcc ctcgatattc accagatctt ctatcgaatc gcgtgtagtt
    10981 ccgtgaatat ccgaaacgat ggctcgttct tcattcagca gcagattgag cagggtagat
    11041 tttcctacgt tagtttcgcc aaccaaagct accggaatac cggttttcaa tacatttcct
    11101 attttgaatg aatcggtgag tgatcttatt ctttcgtgaa tctcaccggc aagtgccgcc
    11161 agttgagtgc ggtcggcaaa ctccacctct tcttccgaaa aatcgagttc gagttcaacc
    11221 aatgaaacaa aatggagtaa tttttctctt aaatttttca attcagctga aaaaccaccg
    11281 cgtatctgat tcatagccat tcggtgtgat gcttcgttgg tagaagctat cagatcggca
    11341 atggcttcag cttgcgataa atccattttt ccgttgcgga atgctctcat ggaaaattct
    11401 ccgggtttag ccagcgaaca gcctttctga ataagcaacc tgagtatttt ctgctgaata
    11461 aacaccgatc cgtgacagga aatttccacc acatcttcgc ccgtaaatga gtgaggagcg
    11521 cggaatactg ttaccagaac ttcgtcaacc tcttgctttt ccttatcggt aatgtagcca
    11581 tagtgaacag agtaaccgga tttatcggct agtttttcat tgtcttttcc aaacctgaaa
    11641 attttatcgc aaatacccgg cgcttcgtag cccgaaaccc tgattacggc tattccaccc
    11701 gatcctggcg gggtagaaat ggcacaaatg gttttttcgt gctctctatt gccctgaatg
    11761 ggtgagtttg atgatagttg gttatcattt tttgtattca tgttctgatg gttttggcaa
    11821 caaaaagtaa attagaaacc aatcaatata ataactatcc gaatgctgtt tttgttttct
    11881 cagcgagatt tcttcaggat ttctgcttca aaatcaaatt ctatccaaaa cggttagaac
    11941 cagtttttcg atatacggtt tatagtcggt attggctgct tccacacgtc tgttggctat
    12001 aatgcagcat acggtcattg ctttgtgccc catcaggcgt gcaagtccgg ctatagccga
    12061 tccttccatt tcgtagttgg taattttgtt cttgccaaaa ctgaaatttt cgattcgctc
    12121 gttcagttca ggttctgcca aatctatccg aagtactctt ccctgaggcc cgtagaaacc
    12181 atttgctgaa atagtaacgc cgcggagcat atcttcttgt ccgattttat tcaccaattc
    12241 ggcgtcggca tctactacgt aaggagcaca aaaatgcgga ttccatttca actgtttttt
    12301 tacttcttct tcgaactcca gatcacaaac tccatctctt ccggcgtaga aattcagcac
    12361 accgtcgaat ccgattgatt tttcggaaat cagaaagctg cccagcggaa tttccggctg
    12421 cataccgccc gatgtgccta ttctgaccag atttagttgt ctgaaaaatg gcttttcggt
    12481 acgggtttcc aggtcgatat ttgccagtgc atccagttcg tttactacga tatctatgtt
    12541 gtcggtgcca attcctgttg atactaccgt caggcgttta cccttgtaag ttccggttat
    12601 ggtattgaat tcacggcttg ataccgagca ttcctgttcg tcaaaatagg atgccactaa
    12661 agctacacgt cccggatcac cgaccagaat tattttatcg gaaatttgtt cgggtttcag
    12721 gtgcaggtga aatatgctgc catcgtcatt gatgatgagc tcggaaggtg gaaaatgttt
    12781 cataatttat gtttttttct taatccttga gtatttgctt tttatttaat caattacaaa
    12841 tatagctatt tttttgaata attatgttca gtttttgatt atgagtgtga aatctattaa
    12901 caggttaatt gatttatata agaaaaaaag agccgacccg tgagtcaact cttttatttt
    12961 ttttcaagca gagtgagtta gcttatcgtt tcttgcggcc gcttttaatc agcgcctgct
    13021 caagcagata ttgtatttgt ccgtttgtgc tgcgaaactc ctctgcagcc cacttctcaa
    13081 tggcttccat cgtttcgtgg tccatgcgca gtacaaagtt cttggtcctg ttttcttttt
    13141 tttctgccac aaaagatatt ttttctagtt atataacgta cctgaattta ttacaggttg
    13201 agcgtttgat tcgccgcaaa gtactaccag cagattgctt accatggctg ctttcttctc
    13261 gtcatccagt tcgataattc cgtctgcaga aagtttatcc agcgccattt tcaccattcc
    13321 tacagcacct tccactattt tctcacgagc ggcaatgatt gcatcggctt gttggcgcct
    13381 tagcatcact gatgcaattt cgggtgcgta agctatgtaa ttaatgcggg cttccaccac
    13441 ttcgatacca gctattttga gcctttcgtt tatttgcgct tcaagaattt cattcatttc
    13501 ttcgcccccc gaacgaagtg taagctggtt ttcggactcg gtattcagca ggtcataagg
    13561 atacattccg gctacaaatc gcaatgcggc atcgctttga atctgcacga aattttcgta
    13621 agctttcatt tttgtgttta cattgtcgta tgagcccgtt ccgggaatgg aaggtgccat
    13681 cgaggcattg tcaatttcga aggatgcctt gtaagtatcc actactttcc ataccaccac
    13741 cataccgatc atgatgggat tacccgtctt gtcgtttact ttgattggtt ctgcgtccag
    13801 attcctggca cgaagcgtca gctttttctt ggcgtagaaa ggatttaccc aaaagaatcc
    13861 gttatctttc accgtaccgc ggtattcgcc gaagaatacc atgaccctgg cttcgttggg
    13921 ttcaatctgc ataaaacctc cccagaaaat ggtgttgatg ataaacaaca cggtgctcag
    13981 aatggcataa aaggtgatag ctccggaact cagtgtttgg gaagataccg cccatacaaa
    14041 cagcagtacg atggcaataa gcaataatag attgagaata atcatgggaa ttcccgggag
    14101 tttaattccg gaaaattttt tttcttgtgt ttccattttt ttattttttg tatagtatca
    14161 aaataatatc acaaagatat agcattgaat gttaaaatcc aaatttttat caaaatattc
    14221 tttatcaaat tcatttttcg ctaacttgcg gcctgtaaaa attaatctac taaatgcaat
    14281 aaacttatga gaatcgttga aaaagtaatt tggggcgtca tcggagccgg taatgtttgc
    14341 gaagtgaaga gtgtgcccgg tatgtacaaa acgccctttt ctgaggtgaa aatggtgatg
    14401 cgccggaatg cagaaaaagc agcagatttt gcccgcaggc acggtatccc gaattcaact
    14461 accgatgcgg atgtgatttt caacgatccc gacattaaca ttatatacgt tggtactcct
    14521 cctgacactc actgtgaata cacactgaga gcagctgcag ccggtaaagc ggtatatgtg
    14581 gaaaaaccga tggcaaacaa cgctgatgaa tgtaaaaaaa tgatagatgc ctgcatgcgt
    14641 gctgaagtgc ctttattcgt ggcttattac cggagggctt tgcccggatt caacagattg
    14701 ggtgaactaa tccattcggg caaaataggt gaggtgaggt ttgtagagat tgaaatgtgg
    14761 cgtgcacccc gttcatacga tcttaatacc gacggaaact ggcgcgtatt tcccgaaata
    14821 tccggtggag gacatttcca cgatttggct tctcaccagc tggattatct ggattcgctt
    14881 ttcggactcg ttacgcaagc taagggtata ggagtgaatc aggcagggtt gtatccggcc
    14941 gacgatcttg tagcaggtac gttcgttttt gaaaacggag tgtgcggaag cggtatgtgg
    15001 tgttttacag ccgataaaag ttctgaaaag gaaaccatta ctatcctcgg aagtaagggg
    15061 catctgcatt tcaatacttt cggaagtccg ctcgaaattc atattaccac gtccgaaggc
    15121 tctgagctaa tcgaaattga acatcccgac catattcaac aaccacttat tcagacagtt
    15181 gtaaatgagt tgcgcggtat cggaacctgt ccgagtacag gaataaccgc ggcaaggaca
    15241 accggagtga tggagaaaat aacatcccgt attgaattga ctgtgaggta atgatgaaaa
    15301 atgcttaaac aaatatcccc gaaccgggag atgatttgac gcaaaaaaaa ggaaatgtca
    15361 ttgaacattt ccttttctgt tagttgcgga ggcccgattc gaacgaacga cctttgggtt
    15421 atgagcccaa cgagctacca ctgctccact ccgcgatgtg ttttgtgact gcaaaggtac
    15481 aaaatatttc ctgattagca aattatttct cttttatttt cttagataat gagcagaatg
    15541 ccctatttcg ggttattttc gggattgaaa atcacaaaat aacagtttaa ttaccaataa
    15601 aataaagaat cggagaagtg ttttgatttt tttttatgtc attattaaaa aaagtcgtat
    15661 atttgcacct cgttttaaaa actgtattta taataaatta attcataaaa aaatttttcc
    15721 atgcaaaaca aaggacttgt aagaattctt gggattgcgc ttgcgctaat ttgtctgttt
    15781 tatctgtcat tcaacctggt gacgatgaga ttcgaaaaca aggcagaatt agcttcgaca
    15841 acagcgaaag aaacttttca aaaaagcaat acttttgcct ctctgactgc tcaggacaaa
    15901 agtattaaat tggatagcat tggcaatcgt gcttattata gttatctgga ttccgtaggc
    15961 aaacaaaaag tgtatttaaa ttatacgctc aaagaatgtc gtgaaaaagg tgttaattta
    16021 ggacttgacc tcaaaggcgg aatgaacgta acgctggaaa tctcagttcc ggatattatt
    16081 cgtgctttgt caaactacaa cacgactcct aattttacca aagcattgca ggtagctacc
    16141 gaaagacaaa agagccgaag cggaacatcc tacctcgatt tgtttgtcca ggcttacaac
    16201 gagattgatc ctcaggcaaa attagccact atcttcagta ctttcgaact gaaagatcag
    16261 atttcgctca gcagcaccaa cgagcaggtc atagctgtgc tgaaaggcga aatagaatca
    16321 gctatcaaca attcattcaa cgtgttacgt acccgtatcg accgtttcgg cgttattcag
    16381 ccaaatatac agaaacttga aaataccgga cgtattttag tggaattacc gggcgtgaaa
    16441 gaacccgaac gtgttcgtaa acttcttcag ggatcggcta atcttgaatt ttgggctact
    16501 tataatctga atgaaattat caacaacctg gttgaagcca ataaagtaat tgcacagctc
    16561 aatattgccg gacctacaat tgattcgacc tctgttactg ctcccgatac tagcaaagta
    16621 gtagctcaga cttcagataa aacggcgaca tcttcaattg acagcctgaa agctgcgctc
    16681 actaccaagt cacagtcagc agcagattcg cttaaaatga tggagcagaa caaaaaagaa
    16741 aatccgctgt ttgccatact tgccgtgaat gtacaaaacg gacaggtaat tcccggtccg
    16801 caggtgggag tggtagaagg aaaagatact gcacttgtca acacttatct caaaatgaaa
    16861 caggtaaaag aagtgttgcc gcgtgacctg gcattccgct ggggcgtaaa tgccattgac
    16921 gaaaatgaca agtactatca gctcatcgca atcaaacaat ctacccgcga tggtcgtgca
    16981 ccacttacag gtagtgtaat taccgatgcc agagatcagt ttggacaaca ttcatctttt
    17041 gccgaagtga gtatgaccat gaatgccgaa ggtgcaaaaa cctgggctcg tatgactaag
    17101 gaaaatatcg gtaagtctat tgccattgct ttggacgggt atatttattc ttacccgaca
    17161 gttcagaatg aaattacagg cggtcaatcc agcattaccg gacgctttac acccgaagaa
    17221 gccaaagact tggccaacgt actgaaatcc ggtaaaatgc ctgcacctgc ccgaatcgtt
    17281 caggaggacg ttgtaggtcc ttcattgggt gaagaggcca tcaacagcgg tatgtggtca
    17341 ttcctggttg cattcctgct tgtaatgctc tacatgattt tatactacgg cttgattccc
    17401 ggaattattg ccgacattgc attgtttatc aacgttttct tcctgatggg aattttagcg
    17461 gcattcggtg cggtgcttac cctgccgggt atagctggta ttgtgctcac gctcgggatg
    17521 gccgtagatg gtaacgtgtt gatatacgag cgagtccggg aggaaatcga atcgggcaag
    17581 aatatgaaga aggcaattca cgatgggttc aacaatgcta tttcggccat tatcgatgcc
    17641 aacgtgacaa cgttgattac cggtattgta ttgtttatct tcggacaggg acctattcag
    17701 ggatttgcca ctactcttat tatcggtatt gtaacttcat tcgtaacatc ggtattcctt
    17761 acacgtatga tgctcgaaat gtacgcaaac cgtgagaatg ccaaacagct gccgtttact
    17821 acaggagcca ccaaaacctg gttccagaac ctttccattg attttatcgg taaaagaaga
    17881 attgcctata tgatttcagg tgcgctcatc cttgtcagta ttcttggttt cggaatacgc
    17941 ggcatgagct atggtatcga cttcagcgga ggacgtaatt atattgttca gtttgaccag
    18001 aaaatacaaa ccgaagaagt gcgtaagtta ctcgaaagca cctttgacgg agcttcaact
    18061 caggtaataa gcatcggtac caataatgat aaggtgcgta tttctacaaa atacaaaatt
    18121 gacgtaacct ccgaaaccat tgatgatgag atttctgcta aactatacga aggattgaaa
    18181 ccaatgctcg gtaatacgag tctggccgac tttacccaaa agaatatagt aaattcgcaa
    18241 aaagtgggac cgaccattgc cgatgatatc aaaacatcgt cagtctgggc tgtaatcgtt
    18301 gccttattac ttgtttcggt ttatattttt atccgcttca acgatttcgg attctccatg
    18361 ggagctatgg cctcattact tcacgacgtt atcgtgatta taggagtatt tgcacttttg
    18421 tggggaatta tgcctttctc catggaaatt gaccagtcgt ttattgcggc catacttact
    18481 tatgtcgggt tctcggtgaa cgatactgtg gtgatcttcg acagaatccg tgaaaatatc
    18541 cggctttatc caaaaagaga cagaaaggaa attataaacg atgcgttgaa cgatacatta
    18601 agtcgtacct tcagtacttc tatgagtgtg tttgttgttt tacttgccat ctttttgttc
    18661 ggtggtgata ccattcgcgg atttgtattc gcgctgctgg taggtactat cctgggggta
    18721 tattctacat tattcgttgc tgtgcccata gctttcgata ttattgaggt tcggaaaaag
    18781 aaaaaagcca gcaaagaact tactgctgtt cctgtaacta aaaaatagag acataaaatt
    18841 ttagtaattt tatattttat atagcaaaga aaccttgtgg agcaattctg caaggttttt
    18901 tttatgtagc ctgtcgctaa atcgaaagca tgatcttact tctgaata
//
LOCUS       DAORKP010000028        18644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_162_length_18644_cov_9.064823, whole genome shotgun sequence.
ACCESSION   DAORKP010000028 DAORKP010000000
VERSION     DAORKP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18644
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_18644_cov_9.064823"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1212
                     /locus_tag="PLN75_02905"
     CDS             427..1212
                     /locus_tag="PLN75_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRG03098.1"
                     /translation="MIVHAGKFVLLSLMLILSYLPVFSQNDPYRAEIGLQTGLNIYSG
                     DVNSIANQDLYFSNLQNMKSGIGGIFRYRFNQRLAIRLGYDFTTAKGNYLYRDAAGTY
                     SAMLNNRLHVMDVRGEFNFFDLENNPYKRFSKRYSPFIFAGFGGMVMPGYKNSEGKNY
                     TFTMPFGVGFKMKFAQRWNLNLQLANHLLLGDNLEGKVQFDNPLPRTEFNPMNHDMLS
                     GFSIGFSYDFWTRNCDCNENSFAAGRKPASHKTVKPKKTEKKR"
     gene            1282..2004
                     /locus_tag="PLN75_02910"
     CDS             1282..2004
                     /locus_tag="PLN75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRG03099.1"
                     /translation="MSFKEKIDSERLPAHIAIIMDGNGRWAKERGYDRVYGHQHGVIS
                     VRECSEGAAEIGIKYLTLYAFSTENWNRSKEEVDALMELLIDTIEKETPTLNKNNIRL
                     LAIGDLERLPEGAKAKLNRCMLETSSNDRMSLVLALSYSSRWEITNAVTQIAERVKAG
                     NLNPEDITDELITQNLTTKNIPDPDLMIRTSGEIRISNFLLWQLAYSELYFTDTHWPD
                     FRKENLYEAIVEYQKRERRFGK"
     gene            2042..4744
                     /locus_tag="PLN75_02915"
     CDS             2042..4744
                     /locus_tag="PLN75_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRG03100.1"
                     /translation="MHYKTLLTLCLLITFGLWVTAQTPATKDSTTVISQDLPPIDYGQ
                     PKEYTIAGIEVEGAEAYDDFVLIGFSGLAVGQKISIPGTEITNAVKKFWAQSMFSDVD
                     ILQTKFENEKVWLTIRLTMRPKVSDITFNGLKKSEIDDITPKIGIQKEKQITPNMSDR
                     AKLEIKRYLDEKGFANADIKVYQKDDPEKPNYVKVDISIDKKEKIKVHKIYVIGNNAL
                     TVRQIDKAMKKTNDNSIQNILRSKKFINEKFEEDKALIEKEYNAHGYRDAYVVSDSIV
                     PFDGKSVDIYLTVDEGNKYYFGDITWIGNTVYPSDYFNSVLGIKKGDIYNHTLLMKRL
                     NEDDDAVIKNYQNKGYLFSNIQPVEVSIQNDTINYELRLSEGKPATINEVTIKGNTRV
                     YENVIRRELFTKPGQLYSQQDIVRSLQYLAQMQHFENEKLYKDVGMEPNEENGTVDVT
                     YNLETKSSDQIEFSAGWGAAGLVGSVGLKFSNFAIQNLFKPETYRIVPQGEGQTFTID
                     ARTNGNYYSSFSLSFLEPWLGGKRPNSLSVSLYYSATSGMSDRYLSSYQNMYSNYGGY
                     GGYGGYGGYGGYGGYGGYGGYGGYGSGYGGYNTSYENEVDKNKYFRMLGGSIGYGKRL
                     KWPDDYFTFYGQLGFQRYMIKNWYQYETNVTNGNFNNFSLSLTASRNSIDNPLFTRRG
                     STFSLGLEITPPYSLINGKDYSSAAMTDAERYKWLEYHKWKFSAKTFTSLLPIDKTPV
                     LMTRAEFAYVGHYNKNARTPFGTYMFGGDGMTGYGGYANEYISMRGYETGQLTPYEYD
                     SNGNARRVGYLYNKFTLELRYPISLEQSATIWALGFLEAGNSFNKLSDYNPFKLKRSA
                     GVGVRIVLPMFGLMGIDWGYGFDSQVGENKPHGSQFHFVIGKEL"
     gene            4933..5448
                     /locus_tag="PLN75_02920"
     CDS             4933..5448
                     /locus_tag="PLN75_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRG03101.1"
                     /translation="MKKIFLTLCLIAGLGFAAQAQKFATVDMGYIMKNIPAYESANEQ
                     LNQVSKKWQAEVDAKMKEVQQLYKNYQTELVFMSDEMKVKREEEIVAKEKAAQELKRS
                     YFGPEGELFKKRESLMKPIQDEVYNAIQELSNTKGLDLIIDKSSAMSAIFVSQKLDIS
                     DEVLKKLGYSK"
     gene            5518..6072
                     /locus_tag="PLN75_02925"
     CDS             5518..6072
                     /locus_tag="PLN75_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRG03102.1"
                     /translation="MIKKIALFMIALLPLGAMAQSLKIGHVNSQEIIALMPEVETMNT
                     QMKEAQDAWEKELLKSREEYYAKIKAFQDAQATMTESIKQARQSELADMEQRISNLNQ
                     AATDDLQKKNQELAAPILDKVKKAMNDVAAENNFTYVFDTTTQTIVYMGPSANDITPL
                     VKKKLNLKEKPAVTSPSALPAAKK"
     gene            complement(6134..6439)
                     /locus_tag="PLN75_02930"
     CDS             complement(6134..6439)
                     /locus_tag="PLN75_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRG03103.1"
                     /translation="MSSIRYEKETVEKMIRLYCRRKHKSAPGELCPRCEATNRYAQEK
                     LDKCPFGDEKGACAECKIHCYKADMRENIRVIMRYAGPRMLLYHPFDYLIHVLKDRK"
     gene            complement(6443..6790)
                     /locus_tag="PLN75_02935"
     CDS             complement(6443..6790)
                     /locus_tag="PLN75_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRG03104.1"
                     /translation="MAKEKLYYSISEVAEMFSINQSNLRFWEKEFKQLKPRRNDKGTR
                     FYTKEDIQLVKQIMYLINEQKLTLEGVRKKLSEKKDSIAKQQELTERLRNIRNELKGL
                     IAQMGSSTEENEA"
     gene            complement(6977..8326)
                     /locus_tag="PLN75_02940"
     CDS             complement(6977..8326)
                     /locus_tag="PLN75_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRG03105.1"
                     /translation="MKVKINYIVLIVFFLILLSPAYTQVTRVIQNQPLKTIEEPDEEE
                     TEPDSTDFDLDEDEDPDSATVIPDDFTSSLDFLLQNWAVDKNVTSNCISQSNPLTDEN
                     QYRERLRKLPHLIEMPYNTAVKSFIDIYTQQRRKQVEYLLGLSDYYFPIFESALEAMR
                     LPLELKYLPIIESALNPKAVSRAGATGIWQFMIGTGKMYGLEVNTLVDERMDPVKSSK
                     VAAEYLKELYSIFSDWHLAIAAYNCGPGNVNKAIRRAGGKRDFWAIYPYLPSETRSYV
                     PIFIAAGYVMNYADKHNICAAKIRIPALTDTIMLNRRVHMEQISSVLNIQMDELRLLN
                     PQYRRDIIPGDIKPYPVCLPHNYANLFIDRRDEIYAYKANTLVDNRRDEVEIPKPVYN
                     PPKRDSKSKFTYHTVRKGQNLGSIAKKYRTTVAKIRKANGMRTSKIKTGQKLKIPKR"
     gene            complement(8356..9048)
                     /locus_tag="PLN75_02945"
     CDS             complement(8356..9048)
                     /locus_tag="PLN75_02945"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRG03106.1"
                     /translation="MKKNFLIYCFLILVAGYGYSQTITGGVITASDSLNISEVTGPVS
                     RAIQPFNDTLVFDTIVDTKREFKPDPMKSVWLGAIFPGYGQIYNRKYWKLPIVYGGFL
                     GFAYAISWNNSRYTSYKNAYIDISDNDETTNYHIKILPRGYTLQNYPGGVTTYQARLK
                     TGMDKFRRYRDLSVILSVGYYTLVLLDAFVDAQLYDFDISQDLVLNVAPARIEYEQNP
                     RSAYGLQCSIRF"
     gene            complement(9151..10029)
                     /locus_tag="PLN75_02950"
     CDS             complement(9151..10029)
                     /locus_tag="PLN75_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRG03107.1"
                     /translation="MAKNIGLGRGLGALMEMDSLGVSGTSSINEVELSLILPNPHQPR
                     SHFDEESLAELASSIKELGVISPITLRKNEDNTYMIIAGERRYRAAKSLGLKTIPAYV
                     KTAADDQVMEMALIENIQREDLNAIEIALTFFRLIQEYKLTQERLSERVGKKRATIAN
                     YLRLLHLPAEIQMGLKNRKIDMGHARAILGIAEPAVQLKLYNLILKNNYPVRMIEEMV
                     RNYAETGNLSGKQPALRQPKLNDLKELGDLKNQLSQIFNTKVQLTCDEKGKGKITIPF
                     ADDEQLMKIMQLIDRI"
     gene            complement(10061..10825)
                     /locus_tag="PLN75_02955"
     CDS             complement(10061..10825)
                     /locus_tag="PLN75_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG03108.1"
                     /translation="MGKTISIANQKGGVGKTTTAINLAASLANLGQKVLLVDADPQAN
                     ASSGLGLDIRRLEFTIYECLIDNVPIAQAIQKTEIDNLSVLPSHIDLVGAEIELLHLN
                     EREHVMENMLKPVKTDYDYVLIDCSPSLGLITVNALTASDSVIIPVQSEYFALEGISK
                     LLNTIKIIKSKLNSKLEIEGFLITMYDNRLRSANLVLAEVKKHFDKLVFDTAIYRNVR
                     LSEAPSYGKPISLYDPDSRGSLNYFDLAKELIDKHK"
     gene            11378..13057
                     /gene="gldG"
                     /locus_tag="PLN75_02960"
     CDS             11378..13057
                     /gene="gldG"
                     /locus_tag="PLN75_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRG03109.1"
                     /translation="MKSVSKSSVILFISIILLIGLSGIFLFRIDLTSDKRFTVSRQTR
                     ELMKSVSQPVKVTVYLTGDLNPGFMRLKKSTTDMLTELNIYSKGKITIELKTPVESST
                     DNKKEIEYSELEKRGMTATSVHDKDKEGKFIQKIIFPWVELSCNGKIIPVNLLKNNPG
                     LSGDENLNISLENLEFELTDAFYRLTKTKIEKIAFLEGHGELNETETYEASKSLSRYF
                     QIDRGVIGTDAGVLNDYKVVIIAKPTNPFSEKDKYVLDQYLMNGGSLLWLIDGVRISE
                     KSLTETGETPAIPYELNLEDMLFRYGVRINPSLVQDVQCALMPVNVAPTGQKPQFEPI
                     PWVFSPLLLTSGSHPVSRNISPVQARFASSLDLVGNDKNTVKKLLLASSGQSHLLPAP
                     GKISMTDLPDVKDRNYFSVSHIPVGVSIEGVFESVFANRIPPVEIVNPAPVKKTSFVT
                     RQIVIADGDIIRNDIQLSGDSIRTLPPGFDRYTSQQFGNNDLIVNSILFLTDNNNRIA
                     LRSRTLPLRLLKKNTSTSELTRLQLINVVLPLLLLLLGGVVYHWIRKRRYR"
     gene            complement(13210..15219)
                     /locus_tag="PLN75_02965"
     CDS             complement(13210..15219)
                     /locus_tag="PLN75_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03110.1"
                     /translation="MKIIKLNKKLMSAIGLLALLVTFNSCNDFLDRPPLSQVGPGVFL
                     NSEADLASYPINYYNSVFPSHGGWGVGIGNYDNGTDNQATANPNLNLYTKGTWLVPNT
                     GSLGFETIRGCNYFLEQVLPKWKAGKISGNTQNINHYIGEMYMIRAMAYFDKLDTYGD
                     FPIVLNTLPDQEEVLIAAAKRAPRNKVARQILSDLDSAILLLSSSSFNKVRITKEAAQ
                     IAKSRVALYEGSFLNYHKGTPRVPGGPGWPGASMDYNKGFSIDIDAEINFFLDQAMAA
                     AKEVADKITLTANSGVTNPPARDKFSGWNQYFEMFGSRDLAKFAEVAMWRQYNVSLGV
                     THGVTIYVERGGNTGYTKSLVDSYLMKNGLPWYAPGSGYQGDLKIMDVKKDRDLRLQL
                     FLAGEEDVRTVKKDADVFGRPSIINIAETRDVTGYRSRKYLNYDPTEAPGSDLTCISG
                     SIVFRAAEAYLNYIEACYMRKKTLDGNADKYWKDLRTRAGVDPDYTKTIAATDMNKEK
                     SDWGAYSGNVLVDATLYNIRRERRNELASEGFRFNDVVRWRAMDQVSNYIVEGFNLWD
                     EAYNDKFYTDPKPGEVTSTGLIADGSSKANVSNKSLSKYLRPYQIVNLSTNLVFNGYN
                     WSKANYLSPIPYRQMQLASPTGDAATSNLYQNPYWPSEANAAALE"
     gene            complement(15232..18393)
                     /locus_tag="PLN75_02970"
     CDS             complement(15232..18393)
                     /locus_tag="PLN75_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03111.1"
                     /translation="MCAITFTGYAQTKTVTGTVLDKQNEPLIGVTVKVQGTTNGTVTN
                     FNGVYSLSNVSADANLEFSYVGMKLQVQKVSGQSVVNVTMDEDTEMLEEVVVVGYGTQ
                     KKVNVTGAVSTVSSKDLESRPVQNVSQALQGVVPGLNLSQTNAGGALNSSMSVNIRGT
                     GSIGDGSAATPLILIDGVEGNMNALNPNDIESISVLKDAASSSIYGARASFGVILITT
                     KSGKTGKTRVSYGGNLRYSNAVQVPEMLDSYRFAQYFNTAAINDGQGAVFSDKALQNI
                     LDYQAYQNGTFKGTLTDENRNQIMYGTVADNNNRWLMYGGANASTNWFAEMYSDWAPA
                     QEHNVNVSGGTEKVTFLVSGNLLDQKGLIRHGEDKFNRYSLNGKMSIKMAPWATLDYN
                     NRWIRENYSRPAYMTGLFFHNIARRWPTNPVLDPNGHYMEGNEILQMEGGGIDKNEKD
                     YLNQQLKLSLEPLADWFVRLEGSYNTTINFNHWDKLPIYGYDAKNQPFAAPWEGGDAG
                     QSAVGESTYKYNFWSVNLYTDYTKSINDHNFKVMAGTNIELMKTRTLGGSKKDLITPL
                     IPTINTATNSTPSLSGSYDQWSTVGFFGRLNYNFKEKYLFEANLRHDGSSRFINDKMW
                     GTFPSFSAGWNIANEDFFKNLNTPISTLKLRASWGQLGNMNTSAWHPWFLSMPFSTGG
                     GSWLVGNVKPNISSAPGIVSSLMTWETIQSSNIGLDWNALNGRFIGSFELFNRRTLNM
                     IGQAVELSSILGTSVPKLNNADMESKGWELELNWRDRISDFSYGLKFVLSDDKQTILR
                     FPNPTKALSNWYEGEVVGDIWGYTTVGIAKSNQEMTDHLANNKPSWGSNWAAGDVMFK
                     DLNGDKAVNSGKSTLDDHGDLSVIGNSNPRFKYGFTVDAAFKGFDFRAFIQGVAKRDY
                     MLSGPYFWGASGGMWQSAAFVEHWDFFRPEGDPLGANLDAYFPRPQFTQSSKNQQTQT
                     RYLQDASYMRLKNIQLGYTLPQNVVKYAGMQTARLYVSVDNLLTVSQITGIFDPELLG
                     GDWGPGKLYPLSRTVSFGVNLNF"
BASE COUNT         5485 a         3922 c         3773 g         5464 t
ORIGIN      
        1 attctccacc tttgcgggca ggacgacaaa accaccgaaa gtcaggcaga aatgaccaga
       61 aaggaaaacg aagcgctggc tcagttgtat tcaatcgaaa cctgaatggt gtgaattttc
      121 agtaagccaa cttcacagtt tactatatta ctttaaagaa ttacattaca tgaaaacgat
      181 tcatcgggtt ttcagctatc atttctactg actcctgtta tggtcattcg cccatcattt
      241 aagtaaccaa aaatgagttg acttttttgc aattatttgt taaattaaac ccgccttttc
      301 agccatacta cctattatta aaaagattat cattactttt gtcagcaaat tagattagta
      361 atacaaaatt tattgctcaa aaatccgttg tacaatatac ttactattca tttatgagtt
      421 gttatcatga ttgtacatgc cggaaaattt gtattattga gtttaatgtt gattctaagc
      481 tatttacctg tgttttcgca aaacgatccc tacagggctg aaatcggttt gcagacagga
      541 ttaaacattt acagcggcga cgtaaattcg attgcaaatc aggatttgta tttctcgaac
      601 ctgcaaaata tgaagtccgg tatcggaggc atatttcgct acaggttcaa ccagcgtctg
      661 gcgatacgtc tgggttacga ttttacaaca gcaaagggta attatttgta cagggatgct
      721 gctggcactt acagtgccat gctgaataat cggcttcacg tgatggatgt tcgcggtgag
      781 tttaattttt tcgatctgga aaacaatccg tacaaaagat tcagcaaaag gtattcaccc
      841 tttatttttg caggattcgg aggcatggtg atgcccggtt acaaaaattc tgaaggcaag
      901 aattatacat tcactatgcc gtttggcgtg ggcttcaaaa tgaaatttgc gcaacgttgg
      961 aatctgaatc tgcaattggc taaccatctg ttgctgggcg acaacctgga gggaaaggta
     1021 caattcgata atccgctgcc aagaacagag tttaatccga tgaatcacga tatgctgtcc
     1081 ggtttcagta tcggttttag ttatgatttc tggacgcgca attgcgattg taacgagaat
     1141 tcattcgcag caggacgaaa accggcttca cacaaaactg taaagccaaa aaaaaccgaa
     1201 aaaaaacggt agatttgtat ttcaacgact attttaaaaa aaccgaaaaa aaacaaaccc
     1261 agtctgctgg ctttacaata catgtctttc aaagaaaaaa ttgactcaga gcggcttcca
     1321 gctcatattg ccatcattat ggacggcaac ggacgctggg caaaagagcg tggttatgac
     1381 cgtgtttacg ggcatcagca cggagtgatt tcggttcgcg aatgctccga aggtgcagca
     1441 gaaattggca ttaaatacct taccctgtac gccttttcga ccgaaaactg gaacagatcc
     1501 aaggaggaag tcgatgcgct gatggaactt ctgattgaca ccatcgaaaa agaaacaccc
     1561 acgctcaaca aaaacaacat ccgtctgctg gctatcggcg acctcgaaag attgcctgaa
     1621 ggcgccaaag caaaactcaa ccgctgcatg ctggaaacaa gcagcaacga cagaatgagt
     1681 cttgtgctgg cactgagcta ctcatcgcgc tgggaaataa cgaatgcagt aactcaaata
     1741 gccgaaaggg tgaaagccgg aaatctgaac ccggaagaca ttacagatga gcttattaca
     1801 caaaatctga ccactaaaaa tatacccgac cccgatctga tgatacgtac aagcggcgag
     1861 ataagaatca gtaacttcct gctgtggcaa ttggcatact ccgaattata ttttacagac
     1921 acacactggc ccgacttcag aaaggaaaat ttatacgaag ctatcgtgga atatcaaaag
     1981 cgtgaaagac gttttggaaa ataaaaaaat aatagaaata agaagatact caacatatta
     2041 catgcactac aaaactttac tgacactttg tttactgatc actttcggtt tgtgggttac
     2101 cgcccaaaca ccagccacca aagattcaac aactgtcatt tcgcaagacc tgccacctat
     2161 tgactatggt cagccaaaag aatataccat tgccggtatt gaagtagaag gcgctgaggc
     2221 gtatgatgac tttgtgctta tcggtttttc agggttggca gtcggtcaga aaatatctat
     2281 tccagggact gaaataacca acgcagtgaa aaaattctgg gcacagtcca tgttttccga
     2341 cgttgatatt cttcagacta agtttgaaaa cgaaaaagtc tggcttacga tccgactgac
     2401 aatgcgtccc aaagtatctg atattacttt caacggcctt aagaaaagcg aaatagacga
     2461 tataactcct aaaattggta ttcagaaaga aaaacagatt actccaaaca tgtctgacag
     2521 ggcaaaactt gaaatcaagc gctacctgga cgagaaagga tttgccaatg ccgatatcaa
     2581 agtgtatcaa aaagacgatc ctgaaaaacc caattacgta aaagtagata tttccattga
     2641 caaaaaagag aaaataaaag ttcataaaat ctatgtaatc ggaaacaatg ccttaactgt
     2701 cagacagata gacaaggcga tgaagaaaac gaacgacaac agtattcaga atattctgcg
     2761 ctcgaagaaa ttcatcaacg aaaaattcga agaagataaa gccctgattg aaaaagaata
     2821 caacgcacac ggctaccgcg atgcctatgt ggtttcagac agcattgtgc ctttcgatgg
     2881 caaatctgtt gatatttatc ttactgtgga cgagggtaat aaatactatt ttggcgacat
     2941 tacctggatt ggtaacacgg tatatccttc ggattatttc aacagtgtac tgggcattaa
     3001 gaaaggcgac atttacaatc ataccttact gatgaaaaga ctcaacgagg atgatgatgc
     3061 cgtcatcaaa aattatcaga ataaaggata tcttttctca aacatacaac cggtagaagt
     3121 atcgattcag aatgacacta tcaactacga attgcgtttg tctgaaggaa aaccggctac
     3181 tatcaacgag gtgactatca agggaaacac gcgcgtttac gaaaacgtga tacgacggga
     3241 gttatttacg aaaccgggac aactttacag tcaacaggac attgtccgtt ctttgcagta
     3301 tctggctcaa atgcagcatt tcgaaaacga aaaactttac aaagatgtcg gcatggagcc
     3361 aaatgaagaa aatggaacag tagatgtaac ctacaacctc gaaacaaaat cgagcgatca
     3421 gattgaattc tcagccggat ggggagcagc cggtctggtg ggcagtgtgg gactgaaatt
     3481 cagcaacttt gccattcaga atctttttaa accagaaaca tatcggattg taccacaggg
     3541 tgaagggcag acatttacca tcgatgcacg aaccaacgga aattattact catccttcag
     3601 cctatcgttt cttgagccgt ggctgggtgg aaaacgtccc aattcacttt ccgtatcact
     3661 ctactattca gctacatcag gcatgagcga tcgttacctc agttcatatc agaatatgta
     3721 ttccaattat ggcggatatg gtggttacgg tggttacggt ggatatggtg gatacggagg
     3781 atatggtggt tatggtggtt atggtggtta cggtagcggt tacggcggat ataataccag
     3841 ctatgaaaat gaggtggata aaaacaaata ttttcgcatg cttggaggct ctatcggtta
     3901 cggaaaacgt ctgaaatggc cggacgatta ctttactttt tatggtcagt taggatttca
     3961 gcgctacatg atcaaaaact ggtatcagta cgaaaccaat gtcacaaacg gaaatttcaa
     4021 caatttcagc ttaagcctca ccgcgagccg taattctatt gataatccgc tgtttacacg
     4081 ccggggctct acattctcac tcggtctgga aattactccg ccttactccc tgataaacgg
     4141 taaggactat tcgagcgccg ctatgaccga tgccgaacga tacaaatggc tggaatatca
     4201 caagtggaaa ttcagtgcaa agacctttac ttcgctacta cccattgata aaactccggt
     4261 actgatgacc cgcgcggaat ttgcttacgt tggacactac aacaaaaacg cacgcactcc
     4321 gttcggtaca tacatgtttg gaggtgacgg aatgacagga tatggcggat atgccaacga
     4381 gtacatttcc atgcgtgggt atgaaacagg ccagctgact ccctatgaat acgattccaa
     4441 tggcaatgcc cgtcgtgtgg gttatctgta caacaaattc acccttgaac tccgctaccc
     4501 catttcactc gaacaatcgg ccaccatctg ggcattggga ttcctggaag caggtaacag
     4561 tttcaataaa ctctccgatt acaatccgtt caaactaaaa agatcggcag gggtcggggt
     4621 acgtatcgta ctgcctatgt ttggtctgat gggtatcgac tggggctatg gatttgattc
     4681 tcaggtgggt gaaaacaaac ctcacggaag ccagttccac tttgtaatag gaaaagaatt
     4741 ataagtaaat gaccgtaccg gcttctgcac actgataaaa tgtactcatt gaatttccgt
     4801 ttcatcacgc gcacaccgat atgataatca accacttaac aaacatgtaa acttttggca
     4861 aagtatttga tataccaata tcacaaatac attatttttt acataagaaa caaattttta
     4921 atctaattga aaatgaaaaa aatatttttg acactctgcc tgattgccgg actgggattt
     4981 gctgctcagg cacagaaatt tgctaccgtt gacatgggct acatcatgaa aaacataccg
     5041 gcttatgaga gcgccaacga acagttaaac caggtgtcca aaaaatggca ggcagaagtg
     5101 gatgccaaaa tgaaagaagt gcagcaactc tacaaaaact atcagacgga gcttgttttc
     5161 atgtcggacg agatgaaagt taaacgcgag gaagaaatag tagcgaaaga aaaagctgct
     5221 caggaactta aacgctccta tttcggacct gaaggggaac tcttcaaaaa acgggaaagc
     5281 ctgatgaaac caattcagga tgaagtttac aacgctattc aggaactcag caataccaaa
     5341 ggactggatc tgataatcga caagtcatcg gcaatgagtg ccatttttgt ctctcagaag
     5401 ctggatatca gcgatgaagt tctcaaaaaa ttgggatatt ccaaataaaa tttataattt
     5461 tgcacaccct tttttcaaaa aatcaagaat aaaaaactca acttaataaa aacaaaaatg
     5521 attaaaaaaa ttgcattatt tatgattgcc ttgttaccat tgggagcaat ggcacaaagt
     5581 ctcaaaatcg gtcacgtaaa ttcacaggaa atcatagccc tgatgcctga agtggaaacg
     5641 atgaatacac aaatgaaaga agcacaggat gcatgggaaa aagagttgct taaatcgcgc
     5701 gaagaatatt atgccaagat caaagctttt caggatgcgc aggctaccat gactgaaagt
     5761 atcaaacagg ctcgtcaatc cgaactggct gatatggaac aaagaatcag caatctgaat
     5821 caggctgcaa ccgacgattt acaaaagaaa aatcaggaac tggctgctcc gattctggat
     5881 aaagtgaaaa aagcgatgaa tgatgttgct gccgaaaaca attttactta tgtttttgac
     5941 actactactc agacgattgt ttacatgggt ccatcggcca atgatattac tccgctggtg
     6001 aaaaagaaac taaatctgaa agaaaaaccg gctgtaactt ctccttcggc tctgccggca
     6061 gccaagaaat aacaatgcct gcataaacaa aaacttaaaa aaagctgacc aaagtaaatt
     6121 ggatcagctt tttttatttc ctgtctttca gtacatgaat caggtagtca aatggatggt
     6181 aaaggagcat acgcggaccc gcataccgca ttatcacacg gatattctct cgcatgtcgg
     6241 ctttgtaaca atgaatttta cattcggcac aggcaccttt ttcatcgcca aacggacact
     6301 tatccagttt ctcctgtgca taccggttgg tggcttcaca ccgggggcac aactcgcccg
     6361 gggctgattt gtgcttcctg cggcagtata gccggatcat tttttctaca gtttcctttt
     6421 cgtaacgtat ggacgacatt ttctatgctt cgttttcttc agtactgctg cccatttggg
     6481 ctatcaaacc tttcaattcg tttcggatat tacggagtct ttcggtgagt tcctgctgtt
     6541 ttgctatcga atctttcttc tcgctcaatt ttttcctcac accttccagt gtcagtttct
     6601 gctcgttgat aagatacatg atttgtttca ccagctgaat atcttctttg gtgtaaaatc
     6661 tcgttccctt gtcgtttcgg cggggtttca gctgtttgaa ctccttttcc cagaaacgaa
     6721 gattggactg attgatggaa aacatttcag ccacttcgga gatggaataa tataattttt
     6781 cttttgccat atttcagcat ttatttttta caggtaaaat aatatgaaac agccattaaa
     6841 cacaaatttt cacccatgag aataaagatg gatggcgtgt tctcccgata aatcgggaca
     6901 ggctatacgt ccttaatttt aaaaaaataa aaaaacttcg tatgaaataa tcagatgatc
     6961 cttattccgg gagtaattac cgttttggaa ttttcagctt ttgtcctgtc tttatttttg
     7021 aagtcctcat tccattggct tttcttattt tggccacagt ggttcggtat ttcttagcaa
     7081 ttgagcccaa attctgtcct tttctgaccg tatgataagt aaatttggat ttcgaatctc
     7141 tttttggcgg attatatacc ggtttgggga tttccacttc atcgcgccgg ttatcaacaa
     7201 gcgtattggc tttgtaggcg tatatttcat ccctgcggtc gatgaaaagg ttggcgtaat
     7261 tatgaggcag gcagaccgga taaggtttga tatcaccggg aataatatcg cgccggtatt
     7321 gaggattgag caggcgaagt tcgtccattt gaatattgag aaccgaagat atttgttcca
     7381 tgtgaacacg tctgttgagc atgatggtat ctgtgagtgc cggaatacga attttagcag
     7441 cacaaatatt gtgtttatcg gcataattca tcacatagcc ggcagcaata aaaataggaa
     7501 catacgatcg ggtttcggat ggtaaataag gataaatagc ccaaaaatcg cgttttcctc
     7561 cggcacggcg tatggctttg ttcacattgc ccgggccgca gttgtaggct gcaatagcca
     7621 gatgccagtc cgagaaaatg gaatagagtt ctttcaggta ttcagccgcc actttggaag
     7681 attttacagg atccattctt tcgtccacca gcgtattgac ttcaagtccg tacatttttc
     7741 cggtaccaat cataaactgc cagataccgg tagctccggc tcttgaaact gccttggggt
     7801 tcagggctga ttcgatgatc ggcaggtatt tcagttcgag cggaagccgc atcgcttcca
     7861 gtgcactttc gaaaatcgga aaatagtaat cactcaaacc cagcaggtat tccacctgct
     7921 tacggcgttg ttgtgtatag atatctataa acgattttac cgcggtatta taaggcattt
     7981 ctatcaggtg aggtagtttt cggagtcttt cgcggtactg attctcatcg gtgagcggat
     8041 tggattgcga tatgcagttg gaggtaacgt ttttgtcgac cgcccagttt tgaagtaaaa
     8101 aatccagcga acttgtaaag tcatccggaa tgactgttgc cgagtccgga tcttcatctt
     8161 catccaaatc aaaatcggta ctatccggtt ctgtttcttc ctcatcgggc tcttcaatcg
     8221 ttttgagggg ctgattttga attacgcggg tgacctgtgt gtaagccggt gaaaggagga
     8281 ttaaaaaaaa gactattaat acgatataat taattttgac tttcattctt gattgtaatt
     8341 aattaggaga gagctttaaa aacggatact gcattgcaat ccgtaagcag agcggggatt
     8401 ttgctcgtat tctattcgcg ccggtgcaac attcagcacc aaatcctgac tgatatcaaa
     8461 atcataaagc tgagcatcca caaacgcatc cagaagtacc agagtataat atcccacaga
     8521 gagtattacg ctcagatccc tgtatcttct gaacttatcc atccctgttt tcagtcgggc
     8581 ctgatacgtt gtgactcctc ccgggtaatt ttgcagggtg tatccgcggg gcaaaatttt
     8641 aatatggtaa ttggtggttt cgtcgttgtc agaaatatcg atataggcat ttttatagga
     8701 tgtgtatcgt gagttgttcc atgaaatggc ataggcaaaa cccaaaaatc ctccgtaaac
     8761 aatgggcagc ttccagtatt tgcggttata aatttgtccg taacccggaa aaatagcacc
     8821 aagccacacg gatttcatgg ggtcaggttt gaattcgcgc tttgtatcaa caatggtatc
     8881 gaaaaccagt gtatcattga atggctgaat tgcacgtgaa accggcccgg ttacttcgga
     8941 aatatttaaa gagtccgaag cggttatcac tccacccgta attgtctgag agtaaccata
     9001 tccggctacc aaaataagga aacaatatat caggaaattt tttttcaatt tttgatgatg
     9061 gttactggcc aattttatct tttattttct gcaaaaaaat gttttcacat cctgtttttt
     9121 tgttgactaa aaagggacaa gcctgaatac ctaaattctg tcgatgagtt gcatgatttt
     9181 catcaactgc tcatcgtctg caaaaggaat tgtaatttta ccttttcctt tctcgtcgca
     9241 tgtgagctgt actttggtat tgaaaatctg actcagctga tttttcaaat cgcccagttc
     9301 tttcaggtcg ttcagcttag gctgtcgcag ggctggctgt ttaccgctca gattacctgt
     9361 ttcggcatag ttccgaacca tttcttcgat catacgtacc gggtaattat ttttcagtat
     9421 caggttgtag agtttcaact ggactgccgg ttctgcaatt ccgagtatgg cccgtgcatg
     9481 tcccatgtca attttccggt ttttcaggcc catttgtatt tctgccggca ggtggagcag
     9541 cctcagatag ttggctattg ttgcccgctt tttacccacc ctttcgctga gtctttcctg
     9601 ggtgagtttg tattcctgta tcaggcggaa aaacgtgagc gcaatttcga tggcattcag
     9661 gtcttcgcgc tggatattct caatcagtgc catttccatc acctgatcgt cggcggccgt
     9721 tttcacataa gcaggtatgg ttttcagacc aagggatttt gctgctctgt agcgacgttc
     9781 gccggcaata atcatgtagg tattgtcttc gtttttacgc agcgtgatgg gtgaaatcac
     9841 tccgagttct ttgattgagc ttgccagttc ggccagtgat tcttcatcaa aatgcgaacg
     9901 gggctgatgc ggatttggca atatcagact gagttccact tcgttgatgg acgatgttcc
     9961 gcttactccg agggagtcca tctccatcag tgcgcccaat cctctgccca aaccgatatt
    10021 ttttgccata tatttatatt aagtttttta tttatccgaa ttatttatgt ttgtcaatca
    10081 gctctttggc caggtcgaaa taattaagtg aaccgcgtga atcgggatca tacagcgaaa
    10141 tgggttttcc gtaactggga gcttcgctca gacggacatt gcgataaatg gccgtatcga
    10201 aaaccagttt gtcaaagtgt ttcttcactt cggccagcac caggttggct gatcgcaacc
    10261 ggttgtcgta catagtgata aggaaacctt cgatttcgag tttcgaatta agtttgcttt
    10321 tgattatctt aatggtattg aggagtttac ttatcccttc gagagcgaag tattcgcttt
    10381 gaaccggaat aatcaccgag tcggaagccg tcagagcgtt gacagttatc agtccgagcg
    10441 aaggcgagca atcaatcagt acatagtcat agtcggtttt caccggtttg agcatgtttt
    10501 ccattacatg ttcccgttcg tttaggtgaa gcagttctat ctcagctcct accaaatcga
    10561 tgtgcgaggg taaaaccgac aaattatcaa tttcggtttt ctgaatggcc tgagcaatgg
    10621 gtacattgtc aatcaaacat tcgtatattg taaattcaag cctgcgtata tccaatccca
    10681 gccccgatga agcattcgcc tgtgggtcgg catcaacgag gagcactttt tggcccagat
    10741 tggccagtga tgcagccaga ttaattgctg tagttgtctt acccactccg cctttctgat
    10801 tggctatcga aatcgttttt cccatgtatt tctgtaatat aattatccga aaatctgtca
    10861 gttatgaagc attaaataag tatcagaact tcatcataca ctctatgcag acgaatagat
    10921 tctttattta aagtgcaaag ataataaaat aaattcagcc attcaattca tggaaaaagc
    10981 agatgggaat atacgttttt ttttgtaact actagccccc caacccccaa aggggggaat
    11041 ttttggagca actaatgtca tttaagcatt taaatttgac agttttttgt aaatattata
    11101 atttttgaac ctacaattgt tctgaaattg ctaaataaat tactaattga agcgctgagt
    11161 agttatgtgt taaactgtta cagttgaaga aaaaccgtat caattgtgta tcatctgatt
    11221 tcaaattcaa tgttggccgt taaaattcta aattcttgaa atatattaga tttcttactt
    11281 cattcgaaat gacagtcagt attttatctt tttactatct ttgcaatagt tatcgcaagt
    11341 ttcagtataa ataatctgcc ggctaactcc tgatcgcatg aaatctgttt caaaatcttc
    11401 tgtcatttta tttatttcga taatcctgct cattggatta tccggaatat tcctcttcag
    11461 aattgacctt acatcggaca aaaggttcac tgtgagccgc caaacgaggg agttgatgaa
    11521 gtcggtgagt caacctgtga aagttaccgt atatctcaca ggcgatctga atcctggttt
    11581 catgcgtctg aaaaaaagca ccaccgacat gctgaccgaa ctgaatattt acagcaaagg
    11641 taaaataacc atcgaactga agactccggt ggaaagcagt acagataata aaaaagagat
    11701 tgaatacagc gaactcgaaa aacggggtat gacagcaaca tctgtacacg acaaggataa
    11761 ggaaggcaaa ttcatacaaa aaatcatatt tccctgggtg gaattatcct gcaacgggaa
    11821 aataattcct gtaaatctgc tgaaaaataa tcccggcctt tcgggtgatg aaaatcttaa
    11881 tatttcctta gaaaacctgg aatttgaact tacagatgcc ttttaccggc tcacaaaaac
    11941 caaaatagaa aaaatagcct ttctggaagg acacggtgaa ctgaatgaaa cagagacata
    12001 cgaagcctcc aaatcactga gccggtattt tcagattgac aggggagtaa taggcactga
    12061 tgccggtgtg ctgaatgatt acaaagtcgt aatcattgcc aaacctacca accccttcag
    12121 cgagaaggac aaatacgtac tggatcagta cctgatgaac ggaggctctt tactctggct
    12181 tatcgatgga gtaagaatct ctgagaaatc actgaccgaa accggtgaaa ctccggcaat
    12241 accttatgaa ctcaacctgg aagacatgct atttcgctat ggagttcgga ttaatccgtc
    12301 gctggtgcag gatgttcaat gtgcattgat gccggtgaat gtagccccta caggtcagaa
    12361 acctcagttt gagccaatcc cgtgggtatt ctcacccctt ctgctcacct cagggtcaca
    12421 tccggtaagc cgaaatatct cacctgtaca agccagattt gcttcttccc tcgatctggt
    12481 gggcaatgat aaaaatacag taaaaaaatt gctgctggcc agctccggac aatcacacct
    12541 gctgcctgct ccgggcaaaa tatccatgac cgatttaccc gatgtgaaag accgcaatta
    12601 tttttccgtg tcacacattc ctgtgggtgt aagtatcgaa ggtgtttttg agtcggtatt
    12661 cgccaaccgg attccgcctg tggaaatagt gaatccggct ccggtgaaaa aaacaagttt
    12721 tgttacacga cagattgtca ttgccgacgg agatatcatc cgtaatgaca tccaactatc
    12781 gggagactcc atccggacat tgccgcccgg atttgaccgg tacacctcac agcaattcgg
    12841 caacaacgat ttaattgtca acagcattct gtttctgacc gacaataata accggattgc
    12901 actccgctca cgaaccttac cactgcggtt gttaaaaaag aatacctcta catctgagct
    12961 tacccgattg cagctgatca atgttgttct tccgcttctc ctactgctcc tcggtggagt
    13021 tgtatatcat tggataagaa aaaggagata caggtaaaaa aataccaatc tgtaaaataa
    13081 taaaggcgtg aaattattct cacgccttta tgtatttttg aatatgtggt tattgataag
    13141 tcatcaataa aaaaatgtat ttgcctgaca aacaattaaa cagacacata ttcacttatt
    13201 tcttctaaat tattccaaag ctgcagcatt tgcttctgaa ggccagtatg gattttgata
    13261 cagatttgag gtggcagcat ctccggtggg tgatgctaac tgcatttgtc tgtacggaat
    13321 tggtgacaaa taatttgctt tgctccagtt ataaccgtta aatacaagat tggttgataa
    13381 gttaacgatt tgataagggc gcaaatattt acttaaactt ttattggata cgttagcttt
    13441 tgaagaacca tcagctatta accctgttga agtaacttca cccggctttg gatctgtgta
    13501 gaacttatcg ttatatgctt cgtcccaaag gttgaaacct tcaacaatgt aattgcttac
    13561 ctgatccata gcacgccatc ttaccacgtc attaaaccgg aaaccttcgc tggctaattc
    13621 gtttcttctt tctcttcgga tattgtataa agttgcatca accagcacat ttcccgaata
    13681 agctccccag tcgctttttt ccttattcat atctgttgcg gctattgttt tggtataatc
    13741 aggatcaaca ccggctctcg ttcttaagtc tttccagtat ttatctgcat taccatcaag
    13801 cgtttttttc cgcatatagc aagcttcaat atagttcaaa taagcctctg cagctctgaa
    13861 aacgatactt ccggaaatac aggttaagtc agaacccgga gcttccgtcg gatcgtaatt
    13921 cagatacttt ctgcttctgt atccggttac gtctcttgtt tcagcaatat tgataattga
    13981 aggtcttccg aacacatctg catctttttt cacagtccga acatcttctt ctccagccag
    14041 aaaaagttga agacggaggt cacggtcttt tttgacatcc ataattttca aatctccctg
    14101 ataacccgaa ccgggtgcat accagggtaa tccgtttttc atcaaatagc tgtcaaccaa
    14161 acttttagta tatccggtat ttccgccacg ctcaacataa attgttacac cgtgtgttac
    14221 cccaaggcta acattatatt gacgccacat tgccacttca gcgaacttag ctaaatctct
    14281 tgaaccgaac atctcaaaat attgattcca accagaaaac ttatctcttg ccggtggatt
    14341 agttactccc gaatttgctg ttaaagtaat tttgtcagcc acttcttttg cggcagccat
    14401 tgcctgatcg aggaagaaat ttatttctgc atcaatatca atgctgaaac ctttgttgta
    14461 atccattgaa gcgcccggcc aacctggtcc accaggtacg cggggagttc ctttatgata
    14521 attcaaaaaa ctaccttcgt acagagcaac tcttgattta gcaatttgtg cggcttcttt
    14581 tgtaattctc actttgttga atgaagaact tgacaacaga agaatagctg aatccaaatc
    14641 ggataaaatc tgacgggcta ccttatttct cggggcacgt tttgcagcag caatcagtac
    14701 ttcttcctgg tcgggtaatg tattcagaac aataggaaaa tctccataag tatccagttt
    14761 gtcaaaatag gccatagcac ggatcatgta catttcacct atgtaatggt tgatgttctg
    14821 agtatttccg gatattttac ctgctttcca ttttggtaaa acctgttcga ggaaatagtt
    14881 gcatccacga atggtttcga agcccaaact acccgtattg ggaacaagcc aggttccttt
    14941 tgtatataaa ttcagattcg gattggctgt tgcctgatta tcggtaccgt tatcataatt
    15001 accaattcct acaccccagc ctccgtgtga tgggaataca gaattatagt agttgatagg
    15061 ataggaagcc aggtcggctt cagaatttag gaacactccg ggacctacct gactaagcgg
    15121 tggtctgtcc agaaaatcgt tacatgaatt aaatgtgaca agcaacgcca gcaaacctat
    15181 tgccgacatg agttttttat ttaattttat tattttcatt gtttctgttt tttaaaagtt
    15241 taagtttacg ccaaaagata ctgttctgct caaaggataa agtttacctg gtccccaatc
    15301 accaccaagc aattcgggat caaagatacc tgtaatttga gatacggtaa gtaaattatc
    15361 cacagataca tacaatcggg ctgtttgcat accggcatat ttaactacgt tttgaggtaa
    15421 tgtataacct agttgaatgt ttttcaaacg catgtaagaa gcatcctgca aatatctggt
    15481 ttgagtctgc tggttttttg aactttgagt aaactgaggt ctggggaaat aagcatcgag
    15541 attggctccc aatggatcgc cttccggacg gaaaaagtcc caatgttcaa caaaagcagc
    15601 tgattgccac attccaccac ttgcacccca gaaatagggt cctgacaaca tataatcacg
    15661 cttggcaact ccctgaatga atgctctgaa atcaaatcct ttgaatgcag catccaccgt
    15721 aaaaccatat ttgaatcttg gattactatt tccgattacc gataaatctc cgtgatcatc
    15781 cagggtagat tttccactat taacagcctt gtcaccgttc aggtctttga acataacatc
    15841 accggcagcc cagttacttc cccaggatgg tttgttgtta gctaaatggt ctgtcatttc
    15901 ctgatttgat tttgctatgc caactgtagt atatccccag atatctccaa caacttcgcc
    15961 ttcataccag ttgctcaatg ctttggttgg gttggggaag cgaagaattg tttgtttgtc
    16021 gtcagataat acaaacttca atccatagct gaaatcgctg attctgtctc gccagtttag
    16081 ttcgagttcc catccttttg attccatgtc ggcattgttc aactttggga ctgaagtgcc
    16141 cagaatagat gacaattcaa ctgcctgacc aatcatattc aatgttctcc ggttaaataa
    16201 ttcgaaagaa ccaataaaac gtccgttaag tgcattccag tccaacccga tattcgacga
    16261 ttgaatagtc tcccatgtca tcaatgaact tactataccg ggtgctgacg aaatattcgg
    16321 ttttacgtta ccaaccagcc aggaacctcc gccggtagag aaaggcatac tgaggaacca
    16381 tggatgccaa gcagaagtat tcatattacc cagctgaccc caggatgctc taagtttcag
    16441 ggtagagata ggagtgttga gatttttgaa gaaatcttcg ttggctatat tccagccggc
    16501 tgagaacgat gggaatgttc cccacatttt atcattgata aatctggaag aaccatcatg
    16561 acgcaaattt gcttcaaaca agtatttttc tttgaagttg taatttaagc gaccaaaaaa
    16621 tccgactgtt gaccattgat catacgaccc ggataaactt ggagtactgt tggtagctgt
    16681 gttaatagtc ggaatcaacg gagtaatcaa atctttcttt gatccaccca gtgtcctggt
    16741 tttcatcaat tcgatgttgg taccggccat cactttgaaa ttatggtcgt tgatactttt
    16801 ggtgtaatcc gtgtaaagat tgacactcca gaaattatat ttgtaagtgc tttctccaac
    16861 tgcagactga ccggcatcac caccttccca gggagctgca aaaggctgat ttttggcatc
    16921 ataaccatat ataggtaatt tatcccagtg attgaaattt attgtagtat tataacttcc
    16981 ttccaacctt acaaaccagt cagcaagtgg ctccagagat aatttcaact gttgattcag
    17041 ataatctttc tcattcttat caattccgcc gccttccatc tggaggattt catttccttc
    17101 catgtagtgt ccgttcggat ctaaaacagg atttgtgggc cagcgacgtg ctatattgtg
    17161 gaaaaacaaa ccggtcatat aggccggacg actgtagttt tccctgatcc atcggttgtt
    17221 gtaatccaat gttgcccagg gtgccatctt aatcgacatt tttccgttta atgaataacg
    17281 gttaaattta tcttctccgt gacgtattaa ccctttttgg tccaatagat ttccgcttac
    17341 aaggaatgta actttttcgg tacctccact tacatttaca ttatgttctt gagctggtgc
    17401 ccaatcggaa tacatttccg caaaccagtt ggtacttgca ttagctccac catacatcaa
    17461 ccagcgattg ttgttatcag ctactgttcc atacataatt tgatttctgt tctcatctgt
    17521 taaggttcct ttgaaagttc cattttgata agcctgataa tcaagaatat tttgcagcgc
    17581 tttgtcagag aaaacagccc cctgaccatc gttgattgca gctgtattaa aatattgtgc
    17641 aaaccggtac gaatcaagca tttcaggtac ctgaacagcg ttggaatacc tgagattacc
    17701 gccgtatgaa actctggttt taccggtttt acccgatttg gttgtaatta aaataactcc
    17761 gaaagaggct cttgcaccgt agatggatga agaggctgca tcttttaata cagatatgct
    17821 ttcaatgtcg ttgggattaa gggcgttcat attgccttca actccatcaa tcaaaataag
    17881 tggagtagca gcagatccat caccgatgga acctgtacca cgtatgttta ccgacatcga
    17941 actgttcagt gcgccacctg cattcgtttg tgataaattt aacccgggta caacaccttg
    18001 cagagcttgt gatacattct gtaccggtct cgattccaga tctttagatg atacagttga
    18061 aacggcacct gtcacattta ctttcttttg tgtgccgtaa cccactacta ccacttcttc
    18121 aagcatttct gtatcttcgt ccatggttac attgacaaca gattgtcctg aaactttttg
    18181 tacttgtaat ttcattccta cgtagctaaa ttctaaatta gcatctgcag aaacattgct
    18241 taaagagtaa actccattga aattagtcac agtaccattt gttgtacctt gtactttgac
    18301 tgtaacccca atgagcggct cgttttgctt gtccaacaca gttcctgtca ctgttttagt
    18361 ttgagcataa ccagtaaaag taattgcaca catcaaaatg gcaagtacac ttctaaaatg
    18421 aaataaaatt tttgattttt tcattctcct tgtttttaat tgttaataaa tagattgttg
    18481 gttagtaatt tagatatatt tttttgtttt cattccattt gatagtcaaa gacaaatatc
    18541 atttttgaag caataataaa gctatttaat aacactactg accgacaaaa aataatgcat
    18601 caaaaaggga agccatctag cagataactt ccctccgata tagt
//
LOCUS       DAORKP010000029        18106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_180_length_18106_cov_8.286965, whole genome shotgun sequence.
ACCESSION   DAORKP010000029 DAORKP010000000
VERSION     DAORKP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_18106_cov_8.286965"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1493)
                     /locus_tag="PLN75_02975"
     CDS             complement(84..1493)
                     /locus_tag="PLN75_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRG03112.1"
                     /translation="MKRKLTFLLLIGVFSLSCSSKSNLSASNAHNKLSKEKEYEVIAM
                     QDSVINFGKRYMSTPYRYGGTTPKGFDCSGFTSYVYRQFGFSLNRASRDQAKQFPSIP
                     KKELQTGDLVFFEGRKQNGIVGHVGIVTERKENGEFQFLHSSIKRGVTVSSSDEPYYA
                     ARYLKGGRVIDSQSTNLLLARKHDATENYSPENEPSKLNTKTKSESKNRIEAVYHTVK
                     KGDNLSKISKKYDVPITTIQNLNGLKSKKLKKGQRLLITEAVAKPDVPLMQIYSIRAD
                     EDEQKEQTVIAETAHSDDLPVQPAKTETKIEEKKTAVSNQTNLQTKISTNSEQKTAQE
                     KTEITTVGLSIPDEQIKKQDSKSSKHKVATGETLYAIARLYGLTVDEVKLLNNLNSNT
                     INAGQELIVERKTESLNRTSVQQKPSREKPTFHIVQRGETLYSISKKYKCTVDNLKKW
                     NKDSDDNIRPGDKIKIITN"
     gene            complement(1615..2586)
                     /locus_tag="PLN75_02980"
     CDS             complement(1615..2586)
                     /locus_tag="PLN75_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG03113.1"
                     /translation="MPIVKTRRLSIGYTKGKNSTLVQSNLNLQLRRGEMVCLIGPNGS
                     GKTTLLRTLAGLQEPLQGDVMIDDKNISEFTQHEKALQIALVLTDKVQIENATVKDIV
                     TLGQHPYTHWIGGLTPEGRKKVDEAIRMVHLDDKSGHYLNELSDGERQRVMIAKALAQ
                     DTPLIILDEPTAHLDLPNRVEVMLLLHRLAHKTQKSILLSTHELDLALQAADRIWLLN
                     QNEGVIYGVPEDLVIDGSFNRVFQSEAYYFNSTNGNFSMNYTLPKKISVSSSDNTRLY
                     WTLRALARAGYSVESGASTTITITKNGWQMEGKLFETIESLLSTLQE"
     gene            complement(2589..3584)
                     /locus_tag="PLN75_02985"
     CDS             complement(2589..3584)
                     /locus_tag="PLN75_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRG03114.1"
                     /translation="MKSRKTILFALLTVLLALLFLADLTFGSIRIPLSETINVFFGKS
                     TNNIYSEILLNFRLPKAITAILAGAALSVAGLMMQTLFRNPLADPYILGVSSGASLGV
                     AIVMMAAAALPVAFISSGWGLVVAAIIGAALVLLLVVGVSYKVQNSVSLLIVGIMFGT
                     IASSLVSVLQYFSNPDAIKLFIMWNMGSLSAVTWEYLQILLPVILAGLLLAFMLTKRL
                     NGLLLGENYARGLGIPITQTRILIVTATGILAGSVTAFTGPIAFVGVAIPHIARGLFR
                     SAKHQYLMPASIVLGASLILICDIISQIPTHTLPINTISALFGAPVIIWIIMKRK"
     gene            complement(3589..4137)
                     /locus_tag="PLN75_02990"
     CDS             complement(3589..4137)
                     /locus_tag="PLN75_02990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRG03115.1"
                     /translation="MIQLLVSDKIKLKQLQIIDATVIFNTIDTQRQYLGEWLPFVEST
                     ITVEDTLNFIHSVSETPESFRELVFCINYCGNFAGLIGLKFNPADKANRRTEIGYWLS
                     NVYQKKGIITESAAVLVNYAFDVLNLNRIAIKCAVGNERSSKIPRRLGFEYEGTERDG
                     ELFPDGHFVDLEVYSMLKKDWK"
     gene            complement(4155..5288)
                     /locus_tag="PLN75_02995"
     CDS             complement(4155..5288)
                     /locus_tag="PLN75_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRG03116.1"
                     /translation="MNKTVFILPFLVLLTLVSCTKKHGEQVATVPLDTLKPEYAQGFS
                     IIEYRNYKEVIVYSPWKNEEIYARYYLVNDENNTTPDNGIKIQTPLKSLASTSVTHLE
                     FLTLLNEIQTITGVCNPILIYNHTVQKNIKAGKITDLGDAFSMNVERTLMLRPQALMM
                     SGFNQADANAVRVQQAGIPVIYNNEWMETSLLGRAEWIKFVAAFYNKEAEADTIFSGI
                     EQRYNDMKAKASKVSRKPKIMSGNNFRGTWYMPGGNSFMGKLFSDAGADYFFANDTTT
                     GSLPLNLETVLKNFAETDFWLNTNYSSIGDLIKSDSKHALFRPVKTGKVYNFNKRLLP
                     TTANDFWESAVARPDLLLGDVIAILHPDILPDWEWVYTEKLKK"
     gene            complement(5403..6860)
                     /locus_tag="PLN75_03000"
     CDS             complement(5403..6860)
                     /locus_tag="PLN75_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG03117.1"
                     /translation="MLDIQKKKSNPFFVLLSLPSTAMGFALSVQISALSWILSTRYGL
                     EIDKIGIVWAAGPIAGIIAQPLVGLVSDKMWFLGGRRRPWIIIGSLLAALMLLALPNL
                     GIIQSIFASGNDPKAGLLAIAIIVALLLDLSINVSLNPTRSLVADCTTMNERSKGYTW
                     MQTISGTFAVLAYFIGGILGNMVLIYVGAAITLLAAIIPALLIEEPRVLNNVSEETKP
                     KSASTGKDIIMSLLPLTGLLIYGVYVLVSKLLGIDFSARVNVGSMSFGIVEFICIIIL
                     IVTSIMVLTNALRIPKNANDYQKILLAHAFTWVGVQTMFIYAFFFLTNKMELGEAAGK
                     VNSLAFFILSLISAILPAFVLNPIAKRIGMVRTHLIAIFIMAIGYSGIFFFARSVPVY
                     YLFIAIAGIGWGSVVSLPFAIMSERVDQTKMGLYMGIFNLSVVLPQMVASFKMGEIIN
                     HSSDKGVVFLICGITLFISGVLWLFVKNDKSIVQK"
     gene            complement(7128..7802)
                     /gene="yihA"
                     /locus_tag="PLN75_03005"
     CDS             complement(7128..7802)
                     /gene="yihA"
                     /locus_tag="PLN75_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRG03118.1"
                     /translation="MKKNNIKNTEKSEQRTKTPPSGGRGAIISAEFTVSNTDYKKCPE
                     GSKPEYAFIGRSNVGKSSLINMLCNKKGLAMTSSKPGKTLLINHFIINNEWYLVDLPG
                     YGYASTGKNMRDQLKKMIESYILEREQLVNLFVLIDSRHEPQQIDLEFMEWLGISGIP
                     FSIVFTKMDKQSATRGKENIESYKAKLLETWENLPPVFLTSSESKLGREELLGYINRL
                     NIDLFD"
     gene            complement(7834..9303)
                     /locus_tag="PLN75_03010"
     CDS             complement(7834..9303)
                     /locus_tag="PLN75_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRG03119.1"
                     /translation="MQSYIVLLIIVAYFAVLLLISQVVSRLSTDNDAFFRANRKSSWW
                     VVAIGMIGTSISGVTFVSVPGMVRDLSFSYMQTVLGFFFGYIVIARVLLPLYYKLNLT
                     TIYTYLDKRFGRSAYKTGASLFLLGKTIGASARLYIVALILHQTVFSTWNVPFWITVC
                     VTVILIWLYTHRSGIKTVIWTDTIQTIIMIAALVLIIFQVVQRINLPAAEIFASLRNS
                     PMTDIFVFDDWHSRQNFWKQFLSGVFIPIVMTGLDQDMMQKNLTIRTLKDAQKNMYWY
                     GIAFIPINFLFLTLGAMLLMLASQNGIALPQTSDEILPLFATQNLGYSVYILFIIGII
                     AAAFSSADSALAALTTSFSVDILQVDKLADKEKAVKIRRKIHIVISLVFVVIILLFRE
                     INDRSIIDAIYTIVSYTYGPLLGLYAFGLFTNRKTNDKLVPFIAVLSPVICYALNIIS
                     KKYWGYSFGYELLMLNGLITFVGLWMFAPQSLKGEVLKD"
     gene            9439..10059
                     /gene="recR"
                     /locus_tag="PLN75_03015"
     CDS             9439..10059
                     /gene="recR"
                     /locus_tag="PLN75_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRG03120.1"
                     /translation="MNQLYSSILLENAVNEFAKLPGIGRKTALRLVLHLLKQSEDEVE
                     LFGTTFIRLRKEIVYCKVCHNISDTEVCHICSNPSRDAQTICVVENIKDVMSVENTRQ
                     YKGLYHVLGGIISPMDGVGPNDLEIESLVKRVQEEGVKEVILALSTTMEGDTTNFYIF
                     RKLSPTGVKITTIARGIAIGDELEYADEVTLGRSIVNRVEFTNSFK"
     gene            10070..10552
                     /locus_tag="PLN75_03020"
     CDS             10070..10552
                     /locus_tag="PLN75_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03121.1"
                     /translation="MELERKIRMVTKSITVTYYVIYLLTIIAVVAIFFMTYTNVEVQR
                     IDALSPLGVKISTTYLIYLLVSIPVALYSFHIYTVKLRKETDEFLKFKKYKKASNIRL
                     WVVGTGLIVGVILVYLLRSQSMIFTSAIAAIALYFSKPSAVKMIRELDLDADPNQMIR
                     "
     gene            10623..11036
                     /locus_tag="PLN75_03025"
     CDS             10623..11036
                     /locus_tag="PLN75_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03122.1"
                     /translation="MIIAVDFDGTIVTHEYPRIGREIPFAIDTLKRLQEEGHHQLILW
                     TVREGDLLNEAVEFCRTRGLEFYAVNNNYPESEVIENQSRKISAELFIDDRNLGGIPD
                     WGVIYRMIMSGKPWSPVSSEFIPDPAPKKKNWFGR"
     gene            11112..13538
                     /locus_tag="PLN75_03030"
     CDS             11112..13538
                     /locus_tag="PLN75_03030"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HRG03123.1"
                     /translation="MKLSRIFFSFILSFFTFITLNAEDKNLIIRYDSPASIWESTLPL
                     GNGRLGMMPDGGTDTENIVLNDITMWSGSVENAMNIQAIDYLPQIRKYLLEGKNLEAQ
                     NLMYSHFKCNGPGSGFGQGKDIPFGCFQMLGNLKITYNYPSAIQKTAVSDYNFALNLN
                     NAIASTVFRKGKTAYTREYFVSHNADVMVIRISSDKKNAVGFSLDLSRPERGNVALKD
                     NVLSMQGQLNDGQNTTRGVQYKTSIRVVSPDGKISSNNGKLQLEDASEALIIVSSSTS
                     LLDKNYLRTVESLLENASSTSYFKLRTEHINKYQEKFNRVSLKLGDDKNAELPASKRL
                     YEFQKNDDPAFAALYFQFGRYLMISGTRENSMPLNLQGLWAKDVQTPWNGDYHLNINV
                     QMNYWPAEVCNLSELHKPLIDFTKSLVPSGEVTANTFYNAEGWVAHVITNPWRFTAPG
                     EHASWGATNTGGAWLCEHLWEHYAFTRDKNYLKSIFPTLTGAARFFLSMMIAEPKNGW
                     LVTAPSSSPENAFYLPENGKEPVYVCMGPTMDVQIITELFKNVLSAAEILSVEDPVID
                     KIKAALPKLPPMQISKEGYLQEWLEDYKEEDPRHRHVSHLYGLYPSNQISNYRTPELA
                     NAARETLNRRGDEGTGWSRAWKVNFWARLHDGNRSYKLLKSLLQPAFATETSKKPAGG
                     TYPNLFCSHPPFQIDGNFGGTAGIAEMLIQSQDGFVELLPALPDKWSKGEFKGLRVRG
                     GAEMDVKWENNKITEAVIRAIASNTIRIKLPEYVSDVTSDSKNMEKESEFLNLRIYTG
                     DRIVLNFRNK"
     gene            complement(13553..15625)
                     /locus_tag="PLN75_03035"
     CDS             complement(13553..15625)
                     /locus_tag="PLN75_03035"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRG03124.1"
                     /translation="MNKKAKSEIINREISWLFFNNRVLQESANPKVPLIERLKFLGIF
                     SNNLDEFFRVRVATLQRMAEMKINAGLKKDNPEAVLKKISRHTSSLQAAVEANYREIK
                     EQLKAEKIYIVDETELNDEQTAFVINYYDKQLEDAITPIMLSGSGKTFPEMQDARIYF
                     FVKLKNSLKPADKKYALIEMPGSEYSRFLVLPQIDDKKYVMFLDDVIRLCLPQLFRSL
                     PYDTFEMYTTKITRDSEMEIEAELGEGIVDKVSKGIKSRRYGSPLRFIFDSNMSDDMK
                     KYFLKKIDFKKSGTIIPGGRYHNFKDLIGFPTLGRTDLVYQKTEGIENEIIKSFDSAI
                     KAIESQDLFLHYPYYSFSQYVQLLREAAIDPDVKSIKITLYRMAHRSKVAKALIFAAR
                     NGKQVSAVIELRARFDEEHNIQWAQRMEEAGINITFGVEGLKIHSKLTLIKKKNGKRI
                     AAVSTGNFHEGNAAVYTDFTLFTANPEITAELEKVFDFIHQPFQMTRYSHLLVSPQEM
                     RKKLNQLIQNEIINAKKGLPAYILCKINHITDPKLIEKLYDAAGAGVKIKLLVRGQLS
                     MVTTQPKLKGNIEIVGIVDKFLEHSRIFIFCNNNTEKYYISSADWMTRNLDQRIEVAI
                     PVYDKKIQQELKNIVEYGLKDNVKARICDGTGENRYKTNDEPPFRSQNELMNHYIKLE
                     NENAEKLV"
     gene            16139..17626
                     /locus_tag="PLN75_03040"
     CDS             16139..17626
                     /locus_tag="PLN75_03040"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRG03125.1"
                     /translation="MNLNPLVAFLKKSPKEFTKADIINYVVANEIKMIDFRYVAGDGR
                     LKTLNFILTDLHYLEQILSTGERVDGSSLFPNFLHAGSSDLYVIPRFSTAFLDPFNEI
                     PTLSMFCSFFNKDGEPMKNSPEYILKKAAQNFTSVTGMNFQAMGELEFYVIGDREDAF
                     PIPNQRGYHEAGPFTKFEQFRRDCMLFIAQAGGMIKYGHSEVGNFTLGDKIYEQNEIE
                     FLVTDVCDAADQLVLAKWIIRSMAYRYNLDVTFAPKITTAKAGSGMHIHTRITKEGVN
                     QYVKDGALSETAKKAIAGYMTCAASLTAFGNMNPTSYFRLVPHQEAPTSICWGDRNRS
                     VLVRVPLGWTGSNNMVAMANPKENPEIQDYSDKQTIEFRCPDGSADIYLLMAGLCVAA
                     RHGFEMENPLEIAGKTYVDVNIHRQENRKMMDSLAQLPASCWDSAGELEKNRAVFEKH
                     GVFDPQIIDDIIRQLKSFNDQHIREEIANNPPKMAEMVDTYFYCG"
     gene            complement(17748..>18106)
                     /locus_tag="PLN75_03045"
     CDS             complement(17748..>18106)
                     /locus_tag="PLN75_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG03126.1"
                     /translation="YLGKMLFSGEEIYKKASVLSGGEKMRCMISRMMLANANVMVLDS
                     PVNHLDLESIQAFNNTLINFKGNVLMSSHDREFIHSVCNRIIELTPKGIIDKQMDYDD
                     YLVSEDVKAVQERMYR"
BASE COUNT         5170 a         3770 c         3805 g         5361 t
ORIGIN      
        1 tgattgttac tatatttgct gataaaaaaa aaaggtaaac ctcaatgcag gcttaccttt
       61 ttttagtaat aaatatttgt caatcagttg gttatgattt ttattttatc tcccggcctg
      121 atattatcgt cagaatcttt attccatttt tttaagttat ccaccgtgca tttgtatttc
      181 ttcgaaatgg aatacagtgt ttctccgcgc tgtacaatat ggaatgtggg tttttcacgt
      241 gagggttttt gctgaacgga agttctattc aacgattccg tttttctctc aacgatgagt
      301 tcctgaccag cgtttatcgt attgctattc agattattca gcagttttac ctcgtcaact
      361 gtcaaaccat acaatctggc aatagcatac agagtttcac ctgtagctac tttgtgtttt
      421 gaacttttgc tgtcctgttt tttgatttgt tcatccggaa tactcaatcc tactgtggta
      481 atttctgttt tttcttgtgc tgttttttgt tccgagttgg tggaaatttt tgtttgcaga
      541 tttgtttgat ttgaaactgc agtttttttc tcctctattt ttgtttcagt ttttgccggt
      601 tgaacaggta aatcgtcaga atgagctgtt tcagcaatta cagtttgctc tttctgttca
      661 tcttcatccg cgcgaatcga ataaatttgc atcagaggca catcgggttt cgctacggct
      721 tcggtaataa gcagtcgttg tccttttttt agtttctttg atttcagacc attcaaattt
      781 tgtatggtgg taataggaac atcgtatttc tttgagattt tcgaaagatt atcgcccttt
      841 tttacggtgt gatataccgc ctcaatcctg ttttttgatt ctgatttcgt ttttgtattc
      901 agtttcgacg gttcattttc aggcgaatag ttttcggttg catcatgttt acgggcaagt
      961 aaaaggttcg tggattgcga atcaatcact ctgccgcctt tcaggtatcg tgctgcgtag
     1021 taaggttcat ccgacgatga aacggtaact ccccgtttga tggaagaatg cagaaattga
     1081 aactccccgt tttcttttct ctcagtcaca atgcccacgt gtcctacaat tccgttttgt
     1141 tttcttcctt caaaaaatac cagatcaccg gtctgaagtt ctttttttgg aatggatgga
     1201 aattgttttg cctgatcgcg tgaagcgcgg ttaagactaa atccaaactg cctgtacaca
     1261 tacgacgtga atcccgagca gtcaaatcct ttgggtgtag taccgccgta tctgtaaggt
     1321 gttgacatat agcgctttcc gaagtttatt acagaatcct gcatggcaat aacttcgtat
     1381 tccttttcct tactcagttt gttgtgtgca ttcgaagctg acaggttcga ttttgaagag
     1441 cacgacaggg agaatactcc tatcagtaaa aggaatgtaa gttttctttt cacgcgctgt
     1501 gtgtgtttgg aatgttaaaa aggaaaaagt ttattttgaa taattgcaaa tataattatt
     1561 ctccgaatac catacagaat gaattttttt tttttaagaa aaaaaagata taatctactc
     1621 ctgaagtgtt gaaagcaaac tttcaattgt ttcaaacaat ttaccttcca tttgccagcc
     1681 gtttttagta atcgttatcg tggttgatgc acctgattcg acgctgtagc cggctcttgc
     1741 cagtgctctg agtgtccagt acaatcgggt attgtcagat gatgaaacgc ttattttttt
     1801 cgggagggtg tagttcatcg aaaaattacc gtttgtgctg ttgaaatagt acgcttcact
     1861 ttgaaaaacg cgattgaaac ttccgtcgat taccagatct tcgggtacac cgtatattac
     1921 gccttcattc tggttcagca accagatgcg gtcagcagcc tgcagggcaa gatccagttc
     1981 gtgagtcgag agcaggatgg atttctgagt tttgtgggcg aggcggtgga gcagaagcat
     2041 cacttccacc cggttaggta aatccaggtg agctgtcggc tcgtcgagaa taatgagcgg
     2101 agtgtcctgt gccagtgctt tggcaatcat tacccgctgg cgttctccat cggaaagttc
     2161 gttcaggtaa tggccggatt tatcatccag atgtaccatg cgaatggctt catccacttt
     2221 tttacggcct tcgggggtaa gtccgccaat ccagtgtgtg tatggatgct gaccgagtgt
     2281 tacgatgtct ttgactgtgg cattttcaat ttgtaccttg tcagtaagca ccagtgctat
     2341 ttgaagtgct ttttcatgtt gtgtaaattc ggaaatattc ttatcatcta tcattacatc
     2401 tccctgcaat ggttcctgca atccggccag tgttctcagg agtgtggttt ttccgcttcc
     2461 gtttggccca atcagacata ccatttcgcc tctgcggagc tgcaggttca ggttgctttg
     2521 taccaaagtg ctgtttttac ctttggtata accgatggat aaccggcgtg tctttacgat
     2581 tggcataact attttctttt cattattatc cagataatta ccggtgctcc gaatagtgca
     2641 ctgatggtat taatcggcag cgtatgagta gggatttgtg aaatgatatc gcagattaat
     2701 atcaatgaag cacccagcac gatgctggcc ggcatcagat actgatgttt tgccgagcga
     2761 aacagtccgc gggcaatgtg aggaatagcc actcccacaa aggcaatggg accggtaaaa
     2821 gccgtaacgg aacctgccag aattcctgtg gctgttacaa ttaaaatccg ggtttgagta
     2881 atcggaattc ctaatccacg tgcataattt tcaccgagca ataatccgtt cagtcttttt
     2941 gtgagcatga atgcaagcag taagcctgcc aggattaccg gcaggagaat ctgcagatat
     3001 tcccacgtga cggcactcag acttcccata ttccacatga taaacagctt gatagcatcg
     3061 ggattgctga aatactgcaa tacgctcacc agcgatgaag ctatggtgcc aaacatgatt
     3121 cctacgatga gtaatgaaac cgaattttgc actttgtatg atacccctac gaccagcaac
     3181 agcaccagtg ctgctccgat aatggctgct acaactaatc cccatccact tgaaataaaa
     3241 gcaacaggca atgcagctgc tgccatcatg acgatagcca ctcccagact cgctccggaa
     3301 ctgactccca gaatataggg atcggccagc ggatttctga aaagggtttg catcatcaat
     3361 ccagcaacag agagagcagc gccggcaaga atggccgtga tggctttggg caaacggaaa
     3421 ttcagcaaaa tctcagaata gatgttattt gtgctttttc cgaaaaatac atttatcgtt
     3481 tcgctgagcg gtatccggat gctgccgaag gtgaggtcgg ccagaaataa aagtgccagc
     3541 agcaccgtaa gcaatgcgaa caatatcgtt ttccgggatt tcatgctgtt atttccagtc
     3601 tttctttaac atgctatata cttcaaggtc aacaaaatga ccatcgggaa aaagttcgcc
     3661 gtcccgttca gtgccttcat attcaaaacc taagcgtctt gggatcttgc tgctgcgctc
     3721 attgccaaca gcacacttga ttgcaatccg gttgagattg agtacatcga aagcgtaatt
     3781 taccaacacg gctgccgact cggtgataat gccctttttc tgataaacat ttgataacca
     3841 gtaacctatt tcagtacgtc ggttagcttt atctgcagga ttgaatttca aacctatcag
     3901 ccctgcaaaa tttccgcaat aattgataca aaaaacgagt tcacgaaaac tttccggggt
     3961 ttcactgacc gaatgaataa aattcagtgt atcttccacg gtaattgttg attcaacaaa
     4021 gggcagccat tcgcccaggt attgcctttg ggtgtcgatg gtgttgaaaa taactgtagc
     4081 atcgattatc tgcagttgtt tgagctttat tttatcagaa acgagaagtt gtatcattat
     4141 ttcggttgta atgattattt tttcagtttt tcagtataaa cccactccca atccggcaaa
     4201 atatcgggat gtaaaatggc aataacatct ccgagcagta aatcgggacg tgctacggca
     4261 ctttcccaga agtcgttggc ggtagtaggc agcaatcgtt tgttgaaatt atatactttt
     4321 cctgttttta ccggacgaaa aagcgcgtgt ttgctgtccg attttatcaa atctccgatg
     4381 gaactgtaat tcgtattcag ccagaaatct gtttctgcaa aatttttcaa aacagtttca
     4441 agattaaggg gcaggctgcc tgtggtggta tcgttagcaa agaaataatc tgctccggca
     4501 tcgctgaaaa gtttgcccat aaaactgttg ccgccgggca tgtaccacgt accgcggaaa
     4561 ttgttgccgg acatgatttt gggttttcgg gaaactttgg aggcttttgc tttcatgtca
     4621 ttataacgct gttctattcc tgagaaaatg gtgtcggctt cggcttcttt gttgtaaaat
     4681 gcggctacaa attttatcca ttcagcccga ccgagcagtg aggtttccat ccactcgttg
     4741 ttgtaaatca ccggaatgcc ggcttgctgc acacgcacag cgttggcatc tgcctgatta
     4801 aatccgctca tcatcagggc ttggggtctg agcatcagag ttcgctctac attcatgctg
     4861 aaagcgtcgc ccaaatcggt aattttgccg gcttttatat ttttttgaac cgtgtggttg
     4921 tatatcagaa tcggattgca aacgcccgta atggtctgta tttcattgag taaggtcaaa
     4981 aattccagat gagtaaccga tgtggaagca agtgatttca gcggagtttg aatttttatt
     5041 ccattatcgg gagttgtatt attttcatca tttaccaggt aataacgggc atatatttct
     5101 tcgtttttcc acggactgta cacgataact tctttgtaat tgcggtattc aatgattgag
     5161 aatccctgag catattccgg tttcaaggta tccaaaggta cagttgcaac ttgctcaccg
     5221 tgtttttttg tacacgaaac gagtgtaagc aaaaccagaa atggaagtat aaataccgtt
     5281 ttgttcatga agcagggctg ttgggacaaa aaaaaaccgc agattttttt tccgcggttc
     5341 tcattgaatt tttgagtgat actttgcggt tttattccac aaagataaca cgattttttc
     5401 aattatttct gtacaattga tttgtcattt tttacaaaca accaaagcac tccggagata
     5461 aataatgtga tgccgcagat aaggaataca acgcctttat cggaagaatg attgataatt
     5521 tctcccattt tgaagctcgc aaccatttgc ggaagcacca ccgagaggtt gaaaatgccc
     5581 atgtacaagc ccattttggt ttgatccacc cgttcgctca tgatggcaaa aggcaacgaa
     5641 accaccgaac cccatccgat acctgcaatg gcgataaaca gataatatac cggaaccgac
     5701 cgggcaaaaa agaaaatacc cgaataacct atggccataa tgaaaatggc aataaggtga
     5761 gtgcgtacca ttccgattct tttggctatc ggattgagta caaaggcagg cagtatggca
     5821 ctgataagac ttaatataaa gaaagccagc gagtttactt ttccggcagc ttcgccaagt
     5881 tccattttat tggtcagaaa gaaaaatgcg taaataaaca tggtttgtac tccaacccat
     5941 gtaaatgcat gtgcaagcag aattttttga taatcatttg catttttcgg tattcgcagc
     6001 gcattggtga gcaccatgat agatgttaca atgagaatga taatacaaat aaattccaca
     6061 atgccaaaac tcatgcttcc aacattgact cgggcagaaa aatcgattcc aagcaatttc
     6121 gaaacgagta cataaacacc ataaataagc aatccggtga gcggaagcag cgacatgatg
     6181 atgtcttttc cggtagaagc tgattttggt tttgtttctt cacttacatt attaagcact
     6241 ctgggctctt caataagtaa agccggaata atggctgcaa gcaaagtgat ggccgctcca
     6301 acatagatga gcaccatatt tcctaatata ccaccgataa aataggcaag aactgcaaat
     6361 gtacccgaaa tggtctgcat ccaggtataa cctttgcttc gctcattcat ggtagtacag
     6421 tcggccacca gcgaacgggt agggttgagg cttacgttga tggaaagatc aagcagcagc
     6481 gccacgataa tggcaatggc cagcaagccg gctttcggat cgtttccgct ggcaaaaata
     6541 ctttggataa ttccaagatt gggcagcgcc agcagcataa gtgcggcgag cagactgccg
     6601 atgattatcc acggacgccg acgcccgccc agaaaccaca ttttgtccga aactaatccc
     6661 acgagtggct gagcaataat acctgcaatg ggtccggcag cccacacaat accgattttg
     6721 tcaatttcga gtccgtagcg ggtggaaagt atccagctca gcgccgatat ttgtacagac
     6781 aaagcaaaac ccatggctgt ggaaggcagg ctgagaagca caaaaaacgg atttgatttt
     6841 ttcttttgaa tgtctaacat ggttatacgt ttattgttaa attgttcaac atttaattgt
     6901 tcgtttctta cgaaaatatc gtgcaatgtt acttaatttt ccagtaaaaa aatgtaatgt
     6961 ataagattta aggaataaaa gagaattgca aacgtttgca aacctatcaa ttgtctgaac
     7021 ttcgattcat aagatatata tgatttttat gaaaataaaa aaggtcgaaa tgacatcatt
     7081 aatcgtgtta tttttttatt ttcagtaaaa atatcaacat aatcacatta atcaaacaaa
     7141 tcaatgttca gacgatttat ataccccaat aactcctctc tacccaactt actctcactc
     7201 gaagtcaaaa ataccggtgg cagattctcc caggtttcaa ggagttttgc tttgtacgac
     7261 tcaatatttt cttttccgcg tgtagccgat tgtttgtcca ttttggtgaa tacgattgaa
     7321 aatggtattc cgctgatacc cagccattcc atgaattcca ggtcgatttg ctgcggctcg
     7381 tgccggctgt caatgagcac aaaaagattc accagctgct cgcgctcaag gatgtagctt
     7441 tcgatcattt ttttgagttg atcgcgcata ttttttccgg tactggcata gccgtaaccc
     7501 ggtaaatcaa ccagatacca ttcgttgttg atgataaaat gattgattaa cagcgtttta
     7561 ccaggtttcg acgaagtcat tgccaaccct tttttattgc aaagcatatt gattaacgac
     7621 gatttcccaa cgttggaacg tccgataaaa gcatactcgg gctttgaacc ctcgggacat
     7681 tttttgtaat cggtgttgct gacagtaaat tcagcagaaa taattgcccc cctaccccct
     7741 gaagggggag ttttggtgcg ctgttcagat ttttctgtat tttttatatt attttttttc
     7801 ataaaaaaat cgggatatca cattaatatt cactcaatct tttaaaactt ccccttttag
     7861 ggattgaggg gcaaacatcc acaatcccac aaaagtaatc aatccattca acatcagcaa
     7921 ttcatatccg aaagaatatc cccagtattt tttgctgata atgttcaatg cgtaacatat
     7981 tacaggcgat aaaacggcta taaaaggcac cagtttgtcg ttggttttcc gatttgtgaa
     8041 aagaccgaaa gcatataatc cgagcagcgg accgtaagtg taggatacaa tggtgtaaat
     8101 ggcatcgatg atgcttctgt cgtttatttc tctgaaaagc aggatgataa caacgaaaac
     8161 cagcgaaatg actatgtgga ttttccttcg gattttcacg gctttctcct tgtcggcaag
     8221 tttgtcaact tgcagaatat caaccgaaaa agaagtagta agtgctgcta atgcggaatc
     8281 ggcacttgaa aaggcggctg ctataattcc gataataaaa agaatgtaaa ccgaatatcc
     8341 taaattttgt gtagcaaaaa gcggcagtat ttcatccgaa gtttgaggca gagcgattcc
     8401 attttgtgaa gccagcatca gcagcatggc gccaagcgtc aggaaaagaa aatttatcgg
     8461 aataaaagcg attccatacc agtacatatt cttttgcgca tctttaagtg tccggattgt
     8521 caggtttttc tgcatcatat cctggtcgag tccggtcatt acgatgggaa tgaaaacgcc
     8581 gctcaggaat tgtttccaga aattctgtct gctgtgccag tcgtcgaaaa caaaaatgtc
     8641 tgtcatcggg ctgtttctca gcgaagcgaa aatttcggca gccggaagat ttattcgttg
     8701 tacgacctga aaaataatca gcaccagcgc cgctatcatt attatggtct gaatggtatc
     8761 agtccagatt acggttttta taccgctccg atgggtgtaa agccagatta gtataacggt
     8821 gacgcatacg gtgatccaaa acggaacatt ccatgtgctg aaaacagtct ggtgcaatat
     8881 cagggcaacg atgtacaagc gcgccgaagc tccgattgtt tttcccagca gaaataacga
     8941 cgctccggtt ttgtaagcgc tgcggccaaa tcgcttgtca agatacgtgt aaatggttgt
     9001 cagattgagt ttataataga gtggaaggag tacacgggca atgacaatat agccaaagaa
     9061 aaaacccagc acagtctgca tatagctgaa actgagatct cttaccatac ccggcaccga
     9121 cacaaacgtg acccccgaaa tggaagtacc gatcatcccg atggcaacca cccaccacga
     9181 agacttacgg ttggcccgaa agaatgcatc gttgtcggta cttaagcggc ttacaacttg
     9241 agaaataagc agtaaaacgg caaaataggc aacgattata agcagtacaa tgtagctttg
     9301 catggaaaat ttttaggact ttggattgat ttctgcatac aaaattaggt aaaaataccc
     9361 gaaaattcgt gtaagcccgc caaattagtg aagatgcaat ttttttgcta aatttgcatc
     9421 gtatttctca acgtaaatat gaaccagctc tattcatcca tactgctcga aaatgctgtc
     9481 aacgaatttg ccaaattgcc cggcattggc cgaaaaacgg ctctgaggct ggttttgcat
     9541 ttgctcaagc aatccgaaga tgaagtagaa ttgtttggaa ccacttttat acggttgcgc
     9601 aaagaaattg tgtactgcaa agtgtgtcat aatatttccg ataccgaagt atgtcacatt
     9661 tgcagcaatc catcgcgtga cgcacaaacc atttgtgtgg tggaaaacat aaaagatgtc
     9721 atgtcggtag aaaacacccg acagtacaaa ggcctttacc acgtgctggg aggaataatt
     9781 tcgcccatgg atggagtagg gcccaacgat ctggaaatag aaagcctcgt gaaacgtgtg
     9841 caggaagaag gtgtaaaaga ggttatcctg gcattgagca caacgatgga gggagacacc
     9901 accaattttt acattttcag aaaattatcg cccaccggag tgaaaatcac tacaattgca
     9961 cgcggaattg ccattggcga cgagctggaa tatgccgatg aagtgacact cggacgctcc
    10021 atagtgaacc gtgtggaatt tacaaattca tttaaataaa aaaacgtata tggaattaga
    10081 acgaaaaatc agaatggtta ccaaatcaat cactgtgaca tactatgtga tttatctttt
    10141 aacaataata gcggtagtgg ctattttctt catgacttat accaacgtgg aagtacaaag
    10201 aatagatgct ttgagtccgc tcggagtgaa aatatccacc acatacctca tttatctgct
    10261 ggtttcaatt cccgtggcgc tttattcgtt ccacatttac accgttaagc tgcgaaaaga
    10321 aactgatgaa ttcctcaagt tcaaaaaata taaaaaagct tccaacatcc ggttgtgggt
    10381 agtaggcaca ggattgattg taggtgtgat actggtgtat ctgcttcgct cgcaatccat
    10441 gatttttact tctgccattg ctgccattgc actctatttc agtaagccat ccgctgtaaa
    10501 aatgattcgt gaactcgatc tggacgccga tccgaatcaa atgatccggt aaaaattttt
    10561 atttacagaa acagaatttg tttttttaaa aatcaaatca aaaacataaa aaccaaccca
    10621 ttatgataat cgctgtagat tttgacggaa ctatagtaac gcatgaatac ccccggatag
    10681 gtcgcgaaat cccgtttgct atcgatacgc tgaagcggct tcaggaagaa ggacatcatc
    10741 aacttatcct ttggacagta cgtgaaggtg atttactgaa tgaagcggta gaattctgtc
    10801 gcaccagggg actcgaattt tatgccgtca acaacaacta ccctgaaagt gaagtgattg
    10861 aaaatcaatc cagaaaaatt tcggccgaac ttttcattga cgaccgaaac ctgggcggaa
    10921 tacccgactg gggcgttatt taccggatga tcatgtcggg caaaccctgg tctccggtat
    10981 cttccgaatt cattccggat ccggctccaa agaagaaaaa ctggtttggc agataatttt
    11041 tgccataaaa ttttattttt ccccattttt ttgaaactaa gtttaaaaac cattatctaa
    11101 ataaaaaagt catgaaactt tcacgcatct ttttttcctt tattctttct tttttcactt
    11161 ttattactct caatgccgaa gataaaaatc tgataataag gtatgattct ccggcctcaa
    11221 tctgggaatc gactttaccg ctcggaaacg gaaggctcgg tatgatgccc gacggcggta
    11281 ccgatacaga aaatattgtg ctgaatgata ttaccatgtg gtcgggcagt gtggaaaatg
    11341 ccatgaacat ccaggcaatt gattacctgc ctcagattcg taaatacctg ctggaaggga
    11401 aaaatctcga agctcaaaac ctgatgtata gtcatttcaa atgcaacgga cccggttcgg
    11461 gttttggaca gggtaaagat atcccatttg gatgttttca gatgttggga aatctgaaaa
    11521 ttacgtataa ttacccttca gccatacaaa aaacggctgt ttcggattat aattttgcgc
    11581 ttaatctgaa taatgccatc gcttccaccg ttttcagaaa aggaaaaaca gcttacaccc
    11641 gcgagtattt tgtatcgcac aatgccgatg tgatggtcat caggataagt tccgacaaga
    11701 aaaacgccgt tggattttcg ctcgatttga gccgtccgga aagaggaaac gtcgcactca
    11761 aagataatgt tctttcgatg cagggacaac tgaacgacgg acaaaataca acccggggag
    11821 tgcagtataa aaccagtatc cgggtagtga gccctgatgg aaaaataagc tcaaacaacg
    11881 gaaaactcca gctcgaagat gcctcagaag ctcttattat tgtttcgtca tctaccagtc
    11941 tgctggataa aaactacctc aggactgtgg aaagtctgct ggaaaatgca agctctactt
    12001 cttatttcaa attacgcacc gagcatatca acaaatatca ggaaaaattc aaccgtgtaa
    12061 gcctcaaact cggagatgat aaaaatgcag aacttcccgc cagcaagcgt ctttatgaat
    12121 ttcagaaaaa cgacgatccg gcatttgcag ctctttattt ccagttcgga agatacctga
    12181 tgataagcgg aacgcgtgaa aacagcatgc cgctcaatct gcagggactt tgggcaaaag
    12241 atgtccagac gccctggaat ggcgattacc atctgaatat caatgttcag atgaattact
    12301 ggccggccga agtatgcaat ttgtccgaat tgcacaaacc gctgattgac tttacaaaat
    12361 cactcgtacc atcgggcgaa gtgacagcca atacgtttta caatgccgag ggatgggtgg
    12421 ctcacgtcat tacaaatcca tggcgattca ccgctccggg cgaacacgcc tcgtggggcg
    12481 caaccaatac gggcggcgca tggctctgcg aacatttgtg ggagcactat gcctttaccc
    12541 gcgataaaaa ttatcttaaa agcatttttc cgactcttac cggagctgca cggtttttcc
    12601 tttcgatgat gattgccgaa ccgaaaaacg gctggttggt tactgctcca tcatcttcgc
    12661 ccgaaaacgc attctacctt ccggaaaatg gtaaggaacc ggtgtatgtg tgtatgggac
    12721 ccaccatgga cgttcagatt atcaccgaat tgttcaaaaa tgtgttgagt gctgccgaaa
    12781 tcctttcggt agaagatcct gtaatagaca aaataaaggc cgctctcccc aaattaccgc
    12841 caatgcagat aagtaaagag ggttatttgc aggagtggct cgaggattat aaagaagagg
    12901 atccccggca caggcatgtt tctcatttat acggtttgta tccatccaac cagatttcta
    12961 attaccgtac tcccgagctt gccaacgctg cccgcgaaac actcaaccgg cgcggcgacg
    13021 aaggtacagg ctggtccaga gcgtggaaag tgaatttctg ggcgcgcctg cacgacggaa
    13081 accgctctta caaactgctg aaaagcctcc tacagccggc atttgccact gaaacatcta
    13141 aaaaaccggc tggaggaact tatcccaact tgttctgttc gcatcctccg tttcagatag
    13201 atggtaactt cggtggtaca gcaggcattg ccgaaatgct cattcaaagc caggatgggt
    13261 ttgtggaatt gcttccggca cttccggata aatggtcgaa gggtgaattt aaaggactgc
    13321 gggtgcgtgg aggagcggaa atggacgtga agtgggaaaa caataaaatt accgaagccg
    13381 taattcgtgc cattgccagc aacaccatca ggataaaact tccggaatat gtatcggatg
    13441 tgacttcaga ctccaaaaat atggagaaag aaagcgaatt cctgaattta cgtatttata
    13501 ctggagaccg tattgtgctt aatttcagga ataagtaaaa atcaataact tttcagacta
    13561 atttttcagc attttcgttt tccagcttaa tgtaatgatt cattagctcg ttttgtgaac
    13621 ggaacggagg ctcgtcgttg gttttatacc tgttttcgcc cgttccgtcg catatacggg
    13681 cttttacgtt gtctttcagc ccgtattcca cgatgttttt cagctcctgc tggattttct
    13741 tgtcgtaaac aggaattgcc acttcaatcc gttgatccag gtttcgggtc atccagtctg
    13801 ccgacgaaat gtagtatttc tctgtattgt tattacagaa aataaaaatt cgcgaatgtt
    13861 cgagaaattt atccactatc ccaactattt cgatgttacc tttcaatttc ggttgcgtag
    13921 tgaccatgga aagttgcccc cttactaaca gtttgatttt tacacccgct ccggctgcat
    13981 catacagttt ttcgatcagt ttcggatcgg taatgtggtt tattttgcac aatatataag
    14041 ccggcaatcc tttttttgcg ttaataattt cattttgaat gagttgattc agttttttgc
    14101 gcatttcctg aggagatacc agtaaatgtg aatagcgggt catctggaag ggttgatgaa
    14161 tgaagtcaaa tactttttca agctcggctg taatttcagg attggcagtg aagagggtga
    14221 aatctgtata aactgctgca tttccttcgt ggaagtttcc ggtactcact gctgctattc
    14281 gtttcccgtt ttttttcttg ataagtgtca gcttgctgtg aattttcagt ccttccaccc
    14341 caaaggtaat atttatgccc gcttcttcca tgcgttgtgc ccactggatg ttgtgttctt
    14401 cgtcaaaacg ggcgcgcaat tcgattactg ccgaaacctg tttcccgttt cgggctgcaa
    14461 atataagcgc tttagccact ttggagcggt gagccatgcg gtaaagtgtg atttttatac
    14521 tttttacatc gggatcgatg gctgcttcgc ggagcaactg tacatattgc gaaaaactgt
    14581 aataaggata atgcaaaaac aaatcctgac tctcaatggc tttaattgcc gaatcaaatg
    14641 atttaataat ctcattttca atgccttctg ttttctgata aacaaggtcg gttctgccca
    14701 atgtaggaaa tccgatcaaa tccttgaaat tgtggtatct tcctccagga ataatagttc
    14761 ccgatttttt gaagtcaatc tttttcagaa agtatttttt catgtcgtcg gacatgttac
    14821 tgtcaaaaat aaaccgcaaa ggagagccat agcgacggct tttaatacct ttactcacct
    14881 tatccactat gccttcgccg agttctgctt caatttccat ctccgaatca cgggtgattt
    14941 tggtggtgta catctcaaaa gtatcgtaag gaaggcttct gaataactgg ggtaggcata
    15001 gccgaatcac gtcatccaga aacatgacat attttttatc atcaatctga ggtaacacca
    15061 gaaagcgcga gtattccgag cctggcattt ctatcagggc atatttttta tcagcgggtt
    15121 taaggctgtt tttcagtttt acaaaaaaat aaatacgggc atcctgcatt tcggggaatg
    15181 ttttcccgga gcctgaaagc atgatgggag taatggcatc ctccagctgt ttgtcgtaat
    15241 agttgattac gaaagcagtt tgctcgtcat tcagttcggt ttcatctact atataaattt
    15301 tttcggcttt cagttgttcc tttatttccc gataattagc ctccaccgct gcctgaagag
    15361 aagatgtatg cctggatatt tttttcagaa cggcttcagg attgtctttt ttcagtcccg
    15421 cattgatttt catttcagcc atgcgctgca gtgtagctac ccgcaccctg aaaaattcat
    15481 cgagattgtt ggagaaaatg cctaaaaact tcaaacgttc tataagaggt actttcggat
    15541 tggccgattc ctgcagcacc cgattgttga aaaataacca gctaatttct ctgttgataa
    15601 tttccgattt tgctttcttg ttcatataat cctgattcgt aaacatttac aaaaatacaa
    15661 aataatttca gaattatttt tcagaataat ttttactgta catttatagc tggtcagaac
    15721 agatctattc agttattatt tctaatgaaa ctgttcaatt atcagtgtaa gtgtttgaat
    15781 gttaaatgat tgtttttcat gaacattaag atgttaaatt atattaaaca gaattttcgc
    15841 aggaatcaaa tgacttttca agtaaataat ttctgacttt tcaattgatt ttcgggaagc
    15901 aaaaattttt tcaacaaaca ttgatttgta atttagtgac ctcaatcagg aaaagaaaaa
    15961 atgaatttta aatcttaatt tataccgaaa tttatgtaaa aatcaactca gaatttttaa
    16021 ttatttcact acctttgtcg tgtttgctgt tgattgttcc tacagcattt ttttttatat
    16081 tgaccgaaaa acaacatcat ttaattactt aaaactgaat attgtggata acttttcaat
    16141 gaatttaaat cccctggtgg catttttgaa gaaatcgccc aaagaattta ccaaagcaga
    16201 tattatcaat tacgtggtag ctaatgaaat caaaatgatt gatttcagat acgttgccgg
    16261 agacggcagg ctgaaaacgc ttaattttat tctcactgac ctgcactatc tggagcaaat
    16321 tctctccaca ggtgagcgag tggatggttc gagcctgttc ccgaattttt tacacgccgg
    16381 ttcgagtgat ttgtacgtga taccccgttt ttcaactgca tttctcgatc cgttcaatga
    16441 aatacctact ttatcgatgt tctgttcatt tttcaacaag gatggcgaac ccatgaaaaa
    16501 ttcgcctgaa tacattctga aaaaagctgc ccaaaacttt accagcgtaa caggaatgaa
    16561 ttttcaggca atgggcgaac tggagtttta cgtcatcggc gaccgtgaag atgcattccc
    16621 gattccgaat caaagaggat atcacgaagc cggacctttt accaaattcg aacaatttcg
    16681 ccgcgattgt atgcttttca ttgcacaggc aggaggaatg attaaatacg gacactctga
    16741 agtgggtaat tttacactgg gtgataaaat ttacgaacag aacgaaattg aatttctggt
    16801 aaccgatgtc tgtgatgctg ccgatcaact ggttttagcc aagtggatta tccgttcaat
    16861 ggcttacagg tataacctgg atgtaacctt cgcaccgaaa atcacaacag ccaaagcggg
    16921 aagcggtatg cacattcata cccgaattac aaaagaaggt gtaaatcaat atgttaaaga
    16981 tggtgcgctc agtgaaaccg caaaaaaagc catagccgga tatatgacct gtgcggcatc
    17041 actcactgca ttcggcaata tgaatccaac gtcgtacttt cggctggtac ctcatcagga
    17101 agcacccaca tccatctgct ggggcgaccg caaccgttcg gtacttgtgc gggtaccact
    17161 cggatggaca ggcagcaaca acatggtggc aatggccaat ccgaaagaaa accctgaaat
    17221 acaagattat tccgataagc aaaccatcga atttcgctgc ccggatggaa gtgccgacat
    17281 ttatctgctt atggcaggtt tatgcgtagc cgcccgtcat ggatttgaaa tggaaaatcc
    17341 gttggaaata gctggaaaaa cgtatgtgga tgtaaatatt catcgccagg aaaatcgtaa
    17401 gatgatggat tcgttggcac aattgccggc ttcgtgctgg gattctgccg gcgaactgga
    17461 gaaaaaccgg gctgtttttg aaaaacatgg tgtatttgat ccacagatta tcgacgacat
    17521 cattcgccag ttgaaatctt tcaatgatca gcatatacgc gaagagattg ccaacaatcc
    17581 acctaaaatg gctgagatgg tggatacgta tttttattgc ggataaagaa cgctgttaaa
    17641 aaaaacaaaa agcggatatc aaactcatga tttccgcttt tttttaatac gaatactatt
    17701 cagcaagtat aaatttttgg gtctgtctgc tgcaaaatca ggtgcattta tctgtacatt
    17761 ctttcctgca ccgctttcac atcttccgaa accaaataat catcataatc catttgtttg
    17821 tcgatgattc cttttggtgt aagttcgatg atacggttac aaaccgaatg gatgaactca
    17881 cggtcgtgtg acgacatcaa cacatttcct ttgaagttga tcagcgtatt gttgaatgcc
    17941 tgaatggatt ccaaatcaag gtgattcaca ggtgaatcaa gcaccatcac attggcattg
    18001 gcaagcatca tacgtgaaat catgcagcgc attttctcac ctccggatag tacacttgct
    18061 tttttgtaaa tttcttcccc gctgaaaagc atctttccca gataac
//
LOCUS       DAORKP010000030        18005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_184_length_18005_cov_9.228301, whole genome shotgun sequence.
ACCESSION   DAORKP010000030 DAORKP010000000
VERSION     DAORKP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_18005_cov_9.228301"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLN75_03050"
     CDS             <1..219
                     /locus_tag="PLN75_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRG03127.1"
                     /translation="KTCYGLRDQIAILSDGNVVPCCIDADAHLLLGNIFSDKFSEILL
                     SARAQRIRKGFEQHRAVEDWCRKCGFRV"
     gene            436..891
                     /locus_tag="PLN75_03055"
     CDS             436..891
                     /locus_tag="PLN75_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HRG03128.1"
                     /translation="MKTAICPISDKRINENTSRGNAFLTVLLLTAYVLTSNLLIPAFL
                     FADFLLRSSDLSKYSPFAFISAKINQWLKIKPKYINAGPKVFSARIGVVFSLSVLISS
                     LLGWQTSVLIFSVIFGICAFLEAVFAFCVACKVYPFVYRLYFKPGIYRQ"
     gene            complement(946..2094)
                     /locus_tag="PLN75_03060"
     CDS             complement(946..2094)
                     /locus_tag="PLN75_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRG03129.1"
                     /translation="MEYNFDEIRSYNPEEVPAAMQRLCEEKQFMKVVSTLFPLMPKEQ
                     LRHKLLSFRNSDDLQKEMVYPFLQYLEANMTTGINLEGVENIDKEKTYLYISNHRDII
                     LDSALLCSKFIEHGMETVEIAIGDNLLIMPWIEELVRVNKSFIVQRGLSARQVLESSA
                     RLSAYLRHVLKEKKNSIWIAQREGRAKDSDDRTQESLLKMFNLGGNSNQVIENLKELN
                     ICPLSISYEYDPCDFLKAKEFQQKRDNPEHKKSPQDDLINMQTGVMGYKGKVVYHIAG
                     IINEELDAITKETSNRNEQIALAAKLIDYHIHSNYTIYPNNKIAYDEIYGDNEFARDY
                     SKSEKEQFEEYLDKQIRKIDLENKDRDFLRLKMLEMYANPLKNQLAAQ"
     gene            complement(2156..2977)
                     /locus_tag="PLN75_03065"
     CDS             complement(2156..2977)
                     /locus_tag="PLN75_03065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRG03130.1"
                     /translation="MLNKNFIQRTVSGAIFVAIVIASVLISPYTFALVFVIITALALH
                     EFYKLTHKPGETEVNAAVGISAGLLLFICSFVYAYELVNFPVYPIYGLYVLLVLIAEL
                     YRKKKNPVNNWAYFILGQVYIALPFSLLNFILFISGYQPILLMAVFITIWVNDTGAYV
                     SGMLLGKHKLFERISPKKTWEGFFGGAFFALLSGYVFSRFIPDISLIQWLIFSELVVI
                     FGTLGDLSESLIKRTEQVKDSGNAIPGHGGILDRFDSMLMVAPVIFIYLKMIIGE"
     gene            complement(3193..5256)
                     /gene="ftsH"
                     /locus_tag="PLN75_03070"
     CDS             complement(3193..5256)
                     /gene="ftsH"
                     /locus_tag="PLN75_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRG03131.1"
                     /translation="MEKRDLNNKPQKSSSGKPKFNLNWLYLLIIFALFSGFFMGDRKQ
                     TKDVSWSVFESYMYKGYIEKIDVFSNKNIVNGVVLKDSAGYVFGKDSAMYAKDRLISA
                     TITSDDAVLQAVTKVREKTGKNIDFNVKADSDYLNIFIMFFLPVIFLVAMFWLMNRRM
                     SGMGGGGSGGIFSVGKSKAQLFDKDNKNRITFKDVAGLQGAKQEVEEIVEFLKNPSKY
                     TELGGKIPKGALLVGPPGTGKTLLAKAVAGEADVPFFSMSGSDFVEMFVGVGASRVRD
                     LFRQAKEKAPCIIFIDEIDAVGRARGKNTNFGGNDERESTLNQLLTEMDGFGSNSGVI
                     ILAATNRADILDKALLRAGRFDRQIHVDLPDVHERKEIFQVHLRPIKIDSTIDVDFLA
                     RQTPGFSGADIANVCNEAALIAARKAKSFVGKQDFLDAVDRIIGGLEKKNKITTISEK
                     KSIAYHEAGHAAISWKLQYANPLVKVTIVPRGEALGAAWYLPEERQLTNREHMLDEMC
                     ATLGGRAAEEVFLNEISTGALNDLERVTKRAYAMVAYFGMSEKLPNLSYYDSSGSEYG
                     FTKPYSEKTAETIDEEARNIVAEQYERAKQILRDNADGHKQLSDLLIEQEVIFAEDME
                     RIFGKRPWESRSDELMRLNEEAIKKQKENGDSTDTPTENNETEKTGEEKPDDYTKETP
                     KEYSK"
     gene            complement(5388..5765)
                     /gene="rsfS"
                     /locus_tag="PLN75_03075"
     CDS             complement(5388..5765)
                     /gene="rsfS"
                     /locus_tag="PLN75_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRG03132.1"
                     /translation="MTKKKKSLENKEIVDKIVEGIQEKKGKKTVVVDLTKLNEAPCSY
                     FVICEGDSNTHVNAIAFSIKDWVKEKINVKPFATDGFENSQWIAMDYGQIIVHVFQRQ
                     YREFYDLEHLWDDADLDFIPDLD"
     gene            complement(5788..6540)
                     /locus_tag="PLN75_03080"
     CDS             complement(5788..6540)
                     /locus_tag="PLN75_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03133.1"
                     /translation="MSRLKHIFFLIFFFCVQLQAQEIPTSTITLKNGETYRGEIVLRT
                     EEMVMLKTKDGKRYQFQTREIEKIGAENILKSEETANAKINRGSFAGMMDAAGGVAFA
                     NATPIGHTPTMNFTLAFGSRNAFGTNAFAGAGAGYVTVAGNESVKIQSFIPLFFQINM
                     PLNNTKISPAIGSKAGYLFSLNDEYKGGAFLNLSGGIIYKLTSITSLYFGLYAEALGI
                     SGTVIENNELGEFTKQGNATVYSLGLKCSFLF"
     gene            complement(6541..7671)
                     /locus_tag="PLN75_03085"
     CDS             complement(6541..7671)
                     /locus_tag="PLN75_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRG03134.1"
                     /translation="MKPFDVYPLFDIEITKGSGCYTYDNEGNEYLDMYGGHAVISIGH
                     SHPYYLQKMSHQMEQLLFYSNSVRNSLQEELAEKLGQASGYEDYSLFLVNSGAEANEN
                     ALKLASFHTGRKKVVAFGKAFHGRTSAAVRVTDNPKIQAPLNDSPEVEFLPLNEVNTV
                     KNALKNRDVCAVIIEGIQGVGGIQTPDSDFLQALRQECTETGTVLILDEIQSGYGRSG
                     KFFAHQYAGIRPDIITIAKGMGNGFPIGGVLISPMFNAVHGMLGTTFGGNHLACAAAI
                     AVLDVMKNENLVTNAANTGAYLIEKMKSFPKIKEIRGKGLMIGIEMEMPAKELRNKLL
                     FEEKVFTGASGTHVIRLLPPLTLTNGDADVFLTRFYHALISL"
     gene            complement(7876..9936)
                     /gene="htpG"
                     /locus_tag="PLN75_03090"
     CDS             complement(7876..9936)
                     /gene="htpG"
                     /locus_tag="PLN75_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRG03135.1"
                     /translation="MAKGNIGVTSDNIFPVIKKFLYSDHEIFLRELVSNAVDATQKLK
                     TLAAVGEYKGELGDLTIRISINKKKKTLTVSDRGIGMTADEVNSYINQIAFSSAGDFL
                     EKYKNDAQAIIGHFGLGFYSAFMVSEKVEIFTQSYREGAVAQHWSCKGDPEYTLEETI
                     KSERGSDIVLHIDDENKDFLEESKIRELLNKYCKFLPIEIAFGKKKEWKDGKEVETDE
                     DNIINNTKPAWTRKPADLKDEDYSTFYHDLYPMTMDEPLFHIHMNVDYPFNLTGILYF
                     PKIKNNIDIQRNKIQLYSNQVFVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSYLQS
                     DSNVKKISAHITKKVADRLNDIFKSDRTQFEQKWDDLKLFIQYGMLSDEKFYERAEKF
                     ALLKNVDGKYFTLEEYKELTAAEQTDKDSNLIYLYATNKDEQYTYIEDARAKGYDVLI
                     MDGQLDIHAISQFEQKSEKTRFVRVDSDVIDKLIQKEETGKVTLNEDQRNALVSVFES
                     QIPAMDKTNFIVTFEQLPATANPVFVTQNEFMRRMKDMSAMQGGGMMSFYGEMPDSYN
                     LVVNTAHPLIEKLLKDEETATEAEVTPLKAQISEMKTRLDSLKETQKGKKDEEIPAGE
                     KEELDSLQKKIDELNENKKTVYRNYAADNKHVRQLIDLALLANNMLKGEALARFVKRS
                     VELM"
     gene            10233..11774
                     /gene="zwf"
                     /locus_tag="PLN75_03095"
     CDS             10233..11774
                     /gene="zwf"
                     /locus_tag="PLN75_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRG03136.1"
                     /translation="MDKTINTHPKGTAEGQALLIFGANGDLSKRKLLPAIFQLHIDGL
                     LPEKFLVIGCGSDLKDEHTFRKDVELHMHIFAKQNVERYPEKFSSFIGMLYYKTVNNK
                     QAEDFEELKKYAYEVTDRNSIPHNFIYYFSIPPFMYEKVGNHLVSCGLNSEKDGWKRI
                     IVEKPFGYSLDSAEELDRKLHEGFQEHQIYRIDHYLGKETVQNIMVTRFANGFFEPVW
                     NRSYVDRVEITAAEKIGVGDRGGYYESSGALRDMIQNHLLQVLAIVAMEPPAVFDSDS
                     IRNETVKVLQALRPIKPEEVEKYVVRGQYSEGKRRGETIPAYRDEKDVVADSTTETFV
                     ALKLYVDNWRWSDVPFYIRSGKNLPVRVTEIVIHLKPAPLQLFKQRCATQQTNMIILR
                     IHPDAGIAIDFGMKIPGAGYKVQNVNMDFHYSDLMQSKIPEAYERLLLDCMTGDSTLY
                     ARADAVHASWKFVDSILNTWNENAEIPLYLYPANTWGPKEADALFGDKRIKWQDQCDK
                     FSCEI"
     gene            11792..12520
                     /gene="pgl"
                     /locus_tag="PLN75_03100"
     CDS             11792..12520
                     /gene="pgl"
                     /locus_tag="PLN75_03100"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRG03137.1"
                     /translation="MLYNKTHINHEPESVAESVARLIVSKVKQKEGTLEHFNLAISGG
                     NTPNILFGLLADEYAGQIDWNTLRIFWVDERCVDPADKESNYGNVHKILLENVPLPPE
                     NIFNIRGDNNPSDEVVRYREVLKKELPEKNGFPVFDMILLGMGDDGHTASIFPDNLEL
                     IHSAQTVEVALHPHSGQYRITLTGNVICNAKQVIFLITGNSKAEIIESILNEKGDFER
                     FPAYYILSGCEAELYLDFEAAERL"
     gene            12604..12966
                     /locus_tag="PLN75_03105"
     CDS             12604..12966
                     /locus_tag="PLN75_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03138.1"
                     /translation="MKRFAFIASLVMVFSVAYSGEKTTIIRDILTEYAAQKVDNRQEL
                     IQFSDQQAKELKQTELNYLLEVQKAENCRCCNTANKIKKSAEKRDIELQKILTREQYL
                     KYEALDKNRIKKHPLRAL"
     gene            complement(13016..14506)
                     /locus_tag="PLN75_03110"
     CDS             complement(13016..14506)
                     /locus_tag="PLN75_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG03139.1"
                     /translation="MKKFLFLSVLFASLLLVFSACKKEEPKTANPVSQFVYDGLSTYY
                     LWADEMKSKEPTAGDVDPKTYFKSVLNATDTENGWSFITDDVESLLAGFSGEPVDFGF
                     SLAFAYANEAKTEYVAIIKYVFPNSPASNAGLQRKDMIGKINGQPVTESNYGILYGSA
                     TANFTTYKNSGSGLVADKTITLTPASIRTDPVLYTNIYNIGGKKIAYLFYTDFIRNYN
                     QSLYDAFSTFRQAGVTDLVLDLRYNHGGSVISAIYLSSLIAPETKVRNKSPLIVMNYN
                     SYLNGIFDKNKWSRTDSLGVYEKPDPDPVAANLNLNKVYIIATSDSYSASELTTFCLK
                     PYMQVVHIGGNTGGKYTASWTLHPYKEEINKYPVVNIYDEASLKADEKDKLKNWAMQP
                     IVAKYTNYKNEDFSNPGYLVPSYALAEGNGYVSNWTQIGDTKDAFLGQAIYLITGDES
                     YKPKQNIPVSRIKASESASVRLSNPNDAAKEAVILDYNRLPKNMLK"
     gene            complement(14740..15582)
                     /locus_tag="PLN75_03115"
     CDS             complement(14740..15582)
                     /locus_tag="PLN75_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRG03140.1"
                     /translation="MKLDKIEAGSFLADGGAMFGVVPKRVWQKRYPCNDDNFCTLMMR
                     CLVIKTDEKLIVIDTGTGVKQLEYLKYYHFKDIADFDTELKKLGYSAAEVTDVIHTHL
                     HFDHCGSSTRFNEDKSEIIPTFPNAAYWVGKAQWENFLNPNVREGDSYFAENMIPVEK
                     AGRLKLVEKDMYICPEVKVRLFNGHTAGQLVPYIHFGAKTVVFTGDVIPFQAALPIAW
                     VSAYDTFPITSMEEKERMLSEAAEKQQILFFEHDAYNECCTVQRVNGKYRVNRTYLLD
                     EIKP"
     gene            15759..17126
                     /locus_tag="PLN75_03120"
     CDS             15759..17126
                     /locus_tag="PLN75_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRG03141.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKDDYEITGSDDEIFEPSLSRL
                     RGHGLLPKETGWFPEKVTNHLNAVILGMHARADNPELLRAKELGIKIYSFPEYLYQQT
                     RSKTRIVVGGSHGKTSTTAMILYVMNKLRFKADYMVGAQIEGFENMVQLSYDSRVAVF
                     EGDEYLTSPIDPRPKFHLYKPHIAILTGIAWDHINVFPTFDNYVDQFRKFVDTMEIQG
                     RLIYYGGDEILNDIAQNCRQDLIVFPYKTPKYEIRNGITYVKTGKDEVALKIFGEHNL
                     QNMEAARLACRQIGIMDDKFYEAISDFTGASNRLQKIVEKPDRVAFKDFAHSPSKLKA
                     TVRAVRKQYPERTLIACMELHTFSSLNSDFLPQYADSMKEADIAYVYYNPEVIHHKRM
                     EELDPDKVKRAFGGENLLVFTDTNLLQEKLKSLNYENSALLLMSSGNFSGINLSEFAG
                     KLLAT"
     gene            17274..17696
                     /locus_tag="PLN75_03125"
     CDS             17274..17696
                     /locus_tag="PLN75_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRG03142.1"
                     /translation="MNKQLLLDKRYMRMARIWAENSYCERRKVGALLVKNKMIISDGY
                     NGTPSGFENKCEDDNNVSKPYVLHAEANAISKVARSHNSSDGATLYVTASPCMECSKL
                     IIQAGIKRVVYGETYRIMDGVELLQRAGIDVVFLENVE"
     gene            17738..>18005
                     /locus_tag="PLN75_03130"
     CDS             17738..>18005
                     /locus_tag="PLN75_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03143.1"
                     /translation="MNKRNIWTIVIIVFSALGGLLLGNVIADRAQNGKNLRDLSGLFS
                     FGNSSSKVNDLLSLIDGQYVDNVDMDSITDEVMADIVSKLDPHSV"
BASE COUNT         4723 a         3876 c         3955 g         5451 t
ORIGIN      
        1 aaaacctgct acggcttgcg cgaccagata gccattcttt ccgatggaaa tgtagtgccc
       61 tgctgcatcg atgccgacgc acatctgctg cttggaaata tttttagtga taaattttcc
      121 gaaatattac tgtcagcacg agcacagcgt atccgaaaag gatttgaaca gcaccgggcc
      181 gtggaagatt ggtgccggaa atgcggtttc cgggtatagt tgatttgccc cctgaaattc
      241 accacctgct tgcggttatt taagaagtgt ttggttgttc ttcccgatag ctatcggtag
      301 ttcggatgtt tggttgtaaa ttgtatatca aacatctgtt ctcccatgtt cttggtataa
      361 aaataccatt ttttggtgat tgcagagtat gtgaagtagt tttaactttg cagagtaaat
      421 aaaaccaatc cgaatatgaa aactgccatt tgccccatat cagataaacg aatcaacgaa
      481 aatacatcgc gtggaaatgc gtttttgact gttcttttac tcactgctta tgtgctgaca
      541 tccaatttgt taattccggc attccttttt gccgattttt tactccgatc gtcagatttg
      601 tctaaataca gcccttttgc atttatttct gctaaaatca atcaatggct gaaaattaaa
      661 ccaaaatata tcaatgccgg acccaaggtg ttttctgcca gaataggtgt tgtttttagc
      721 ctttcggtac tgatttcatc actgcttggc tggcaaacaa gtgtattgat cttttctgtg
      781 atatttggaa tttgcgcctt tttggaagcc gtttttgcat tctgcgttgc ctgtaaggtt
      841 tatccttttg tttaccgctt atacttcaaa ccgggaattt accgacagta aataaatttc
      901 cctgataaaa tgagagagac tcgtatgtgt gagtctctct catttttatt gtgcagccag
      961 ttggtttttc agcggattgg catacatctc cagcattttt agtcgtagaa aatcgcggtc
     1021 cttattctcc aggtcaattt tcctgatttg cttgtcgagg tattcttcga actgctcttt
     1081 ttcggatttt gaataatctc gggcaaattc attgtcaccg tatatctcat cataggcaat
     1141 tttgttgttt gggtaaatgg tgtaattgct atggatgtga taatcaatca gtttggcagc
     1201 cagtgcgatt tgttcgtttc tgttggaagt ctcttttgta atggcgtcca actcttcatt
     1261 aataattcct gcgatatgat acaccacttt tcctttataa cccatcacgc cggtttgcat
     1321 gttgatcaga tcgtcctgcg gcgacttttt atgttcggga ttatcccgtt tttgttgaaa
     1381 ttccttggct ttcaggaaat cacaggggtc gtactcatac gaaatgctta acgggcagat
     1441 attcagttcc ttcagatttt caataacctg attggagttg ccgcccaaat tgaacatttt
     1501 cagcaggctt tcctgcgtac ggtcgtcgga gtctttggct cttccttcgc gctgtgctat
     1561 ccagatagag tttttctttt ccttcagcac atgacgcagg tatgccgaaa gccttgcgga
     1621 actttcaagt acctgacgtg ccgagagtcc acgctgtacg atgaaacttt tatttacgcg
     1681 caccagttct tcaatccagg gcatgataag cagattgtcg ccgatagcaa tttccacagt
     1741 ttccattccg tgttcgataa atttgctgca cagcagcgcc gaatcgagta tgatatcgcg
     1801 gtgattggat atgtacagat aggttttctc tttgtcaata ttttccactc cttccagatt
     1861 gattccggtt gtcatattgg cttccaggta ttgcagaaat ggataaacca tctctttttg
     1921 caggtcgtcg gagtttctga aactcagaag tttatgtctc agctgttctt tgggcatcaa
     1981 cgggaacagc gtactgacca ctttcataaa ttgtttttct tcgcacagac gctgcatggc
     2041 tgccggtact tcctccgggt tgtaggagcg tatttcgtca aaattatatt ccattggtaa
     2101 tgagtttatt agtcggatta aatgattggt aaatcgattc gtgggcgtac agtcattatt
     2161 ctcctataat cattttcaaa tagatgaaaa taaccggtgc taccatcagc atgctgtcaa
     2221 aacggtccag aatgccgcca tgtccgggta tcgcattgcc cgaatctttc acctgctccg
     2281 ttcgtttgat aagcgattcg gagaggtcgc ccagcgtacc gaaaatcacc accagttcgg
     2341 agaaaatgag ccactgaatg aggcttatat cgggtataaa gcgcgaaaaa acatagccgg
     2401 acaacaaggc aaaaaatgca ccgccgaaaa atccttccca ggtctttttc ggtgaaatcc
     2461 gctcaaaaag tttatgctta ccgagcagca tgccgcttac gtaggcaccg gtatcgttca
     2521 cccagatggt gatgaatact gccatgagca gaatgggttg atatccgctg ataaagagaa
     2581 tgaaattgag cagcgaaaat ggcagggcaa tgtacacctg accgagaatg aaatatgccc
     2641 agttgttcac gggatttttt ttcttccgat acaattcggc aatcagcacc agaagtacgt
     2701 ataatccgta tatgggataa accggaaagt tcaccagctc gtaggcatag acaaaagaac
     2761 agatgaaaag cagcaaacct gctgaaatac ccacagctgc atttacttcg gtttcgcccg
     2821 gtttgtgggt gagtttgtaa aactcgtgca gggcaagcgc cgtaatgatt acaaaaacaa
     2881 gcgcaaaagt atagggtgaa atcaacactg atgcaatgac aattgcaaca aaaatagctc
     2941 cgctgacggt acgctgaatg aagtttttgt ttagcatgtt tcgtgtcgga attttgtgaa
     3001 ttttcgtacg gactaattgc tatgcaaaga tattaaaaga tttgtcattt caaagtaaaa
     3061 tccgtgttac tttgcgctta ctgtttgcaa aatattcgga tgatactgtt atgatttttg
     3121 gatgaaaaaa aaacaggctt tccaattgaa gcctgttttc tgaggttgtg atagtttttt
     3181 ttgaaattca ccttatttgg aatattcttt tggagtctct tttgtatagt catccggttt
     3241 ttcttcgccg gttttctccg tctcgttatt ttcggttggt gtgtcggtag agtctccgtt
     3301 ttccttttgc tttttaatgg cttcctcgtt cagccgcatg agttcatcgc ttcgcgattc
     3361 ccaggggcgt ttgccgaata ttcgttccat gtcttcggca aaaatcactt cctgctctat
     3421 cagcaggtcc gagagttgtt tatgtccatc ggcattgtct cgtaaaatct gttttgcccg
     3481 ttcatattgt tcagcaacta tattccgtgc ttcttcgtcg atggtttcag ctgttttttc
     3541 actgtaaggt ttcgtaaaac cgtattcgct gcccgacgaa tcgtagtaac tcaggttcgg
     3601 aagtttttcg ctcatgccga agtatgccac catcgcgtaa gcgcgtttgg taacccgttc
     3661 gaggtcgttc agtgcaccgg ttgatatttc attcaggaaa acttcctcgg ccgcacgtcc
     3721 gcccagcgtg gcgcacattt catccagcat gtgttcgcgg ttggtcagtt gccgttcttc
     3781 gggcagatac caggctgctc cgagtgcctc gccgcgaggt acgatggtca ctttcaccag
     3841 cggattagca tactggagtt tccagcttat ggcagcatgt ccggcttcgt gataggcaat
     3901 ggattttttt tcagaaatgg ttgtaatctt gtttttcttt tccaaaccac cgattatccg
     3961 atccacggca tccagaaaat cctgtttacc tacaaaagat ttggctttgc gggctgcaat
     4021 caacgcagct tcgttgcaca cattggcaat atcggctccg gaaaaacccg gagtctgtcg
     4081 tgccagaaaa tccacgtcga tagtgctgtc gatttttatc gggcgcaggt gaacctggaa
     4141 tatctcttta cgttcgtgta catcgggcag gtccacgtga atctgacggt cgaaacgtcc
     4201 ggcacgcagc aatgctttgt ccagaatgtc ggcacggttg gtggcggcca gaataatgac
     4261 tccgctgttc gatccgaatc cgtccatctc ggtaagcagc tgattcagcg tgctttcacg
     4321 ttcgtcgttg cctccgaagt tggtgttttt gccgcgggca cgtcctacag cgtcaatttc
     4381 atctataaaa atgatgcagg gtgctttttc cttggcttgc cggaacaaat cacgaacccg
     4441 cgaagctccc acgcccacaa acatttccac aaaatcggaa ccggacatgg agaaaaacgg
     4501 cacatcggct tcgccggcca ctgctttggc aagcagcgtt ttaccggtac ccggaggacc
     4561 tacaagcagg gcgcctttgg ggatttttcc acccaattcg gtgtacttgg aagggttttt
     4621 aaggaattca acaatttctt ccacttcctg cttggcaccc tgaagcccgg ctacgtcctt
     4681 gaaagttatc cggtttttat tgtccttgtc aaaaagctgg gctttcgact taccaacact
     4741 gaatataccg ccgcttccgc cgccgcccat tcccgacatt ctccggttca taagccagaa
     4801 catggctacc agaaatatta ccggaaggaa aaacatgatg aaaatattca ggtaatcgct
     4861 gtcggctttt acgttgaaat cgatgttttt accggttttt tctctgactt tggtcactgc
     4921 ctgcaatacg gcatcgtccg aagtgatggt ggcggatatc agcctgtctt tggcgtacat
     4981 ggccgaatcc tttccgaaaa catagccggc agagtctttt aatacgactc cgttcacgat
     5041 gtttttattg gagaatacat ctattttttc gatatatcct ttgtacatgt aagattcaaa
     5101 taccgaccag gaaacgtctt tggtttgctt gcggtcgccc atgaaaaatc cgctgaaaag
     5161 ggcaaatatt attaacagat aaagccagtt cagattgaat tttggtttac ctgacgatga
     5221 tttttgaggc ttgttgttta ggtctctttt ttccatttag ttatctctct tttttcgaat
     5281 tgtatattaa aataaatttg cctgtaccgt agtggtagag acaaaaatgg ttttctactc
     5341 atttaacgtt tgagtattgc catttgttta agaaaataaa gctttcatta atccagatca
     5401 gggataaaat ccagatcggc atcgtcccac aggtgttcca gatcgtaaaa ttcgcggtac
     5461 tgacgctgaa aaacatgtac aataatctgg ccgtagtcca tggcaatcca ttgcgagttt
     5521 tcgaaaccgt ctgtggcaaa gggctttaca ttgatttttt ctttcaccca gtctttgatg
     5581 gaaaaagcta tagcgttgac gtgggtattc gaatcgcctt cacaaatcac aaaatagctg
     5641 catggggctt cattcagctt ggtgaggtca accacaaccg ttttctttcc ctttttttcc
     5701 tgaatgcctt ctactatttt atcaacaatt tctttgttct caagcgattt tttctttttt
     5761 gtcatgtttg atgttgtgtt ttataattta aaaaaggaat gaacatttca atcccaggct
     5821 gtaaacagta gcgtttccct gttttgtaaa ttctcccagt tcgttgttct caattactgt
     5881 gcctgatatt ccgagcgctt cggcgtagag cccgaaataa agcgatgtga tggatgtcag
     5941 tttgtagatg attcctcccg ataagttgag aaatgcgcct cctttgtact cgtcgttcaa
     6001 tgaaaacagg tagccggctt tacttccgat tgcaggactg attttggtgt tgtttaacgg
     6061 catgtttatc tgaaaaaaca atggtataaa tgattggatt tttacacttt cgttgcctgc
     6121 cactgtaaca taaccggctc cggctcctgc aaaggcattt gtaccgaacg cattgcgcga
     6181 cccaaatgca agggtaaaat tcattgtcgg cgtatggcca atcggtgttg cgttggcaaa
     6241 agccactccg cccgcagcat ccatcattcc ggcaaaacta ccccggttaa ttttcgcgtt
     6301 ggcagtttct tcacttttca ggatgttttc cgcaccaatt ttttcaattt ctctggtctg
     6361 aaactggtag cgttttccgt ctttggtttt gagcatgacc atttcttccg ttcgcagcac
     6421 tatttctcca cggtatgttt ctccgttttt gagggtgatg gtgctcgtgg gaatttcctg
     6481 cgcctgaagc tgtacgcaaa aaaagaaaat caggaagaaa atgtgtttta atctgctcat
     6541 ttacagcgat attaaagcgt gataaaatct tgtcagaaag acatcggcat cgccgttggt
     6601 gagcgtaaga ggcggtagca ggcgtataac gtgtgttccg ctggctcctg taaacacttt
     6661 ttcttcgaaa agcaatttgt ttcgaagttc tttggcgggc atttccattt cgatgcctat
     6721 catcagtcct ttaccccgaa tttcttttat tttcggaaaa cttttcattt tttctatcag
     6781 ataagcccct gtattagcgg catttgtcac cagattttcg tttttcatca catcaagtac
     6841 ggcaatggcc gccgcacatg ccaggtgatt gcctccgaaa gtggttccaa gcattccgtg
     6901 tacggcattg aacatcgggc tgatgagcac tcctccgatc gggaaaccgt ttcccatgcc
     6961 tttagcgatg gtgattatgt ccggacgaat gccggcgtat tgatgagcga aaaattttcc
     7021 gctgcgtccg tagcccgact gaatttcatc cagaataagt accgtgccgg tttccgtgca
     7081 ttcttggcgg agtgcctgca ggaaatctga atccggagtc tgaatgccgc ctacgccttg
     7141 tataccttca atgatgactg cacatacatc gcggtttttc aaagcgtttt tcacggtgtt
     7201 aacctcgttg agcggaagaa attccacttc ggggctgtca ttcagcgggg cctggatttt
     7261 cggattgtca gtcactctca cggctgccga tgtgcgtccg tggaaggctt tgccgaatgc
     7321 caccactttt ttcctgccgg tgtggaatga tgccagcttc aacgcatttt cgttagcttc
     7381 ggcacctgaa ttgaccagaa acaacgagta atcctcataa ccggatgcct gcccgagttt
     7441 ttcggccagt tcttcctgca gcgaattgcg gacagaattg gagtagaaca gcagttgttc
     7501 catctgatga ctcattttct gcaggtagta tggatgtgag tgcccaatgg aaatgacggc
     7561 gtggccaccg tacatatcca ggtactcatt tccttcgtta tcgtaggtgt aacatccgct
     7621 tccttttgta atttctatgt caaagagagg ataaacatca aatggtttca ttcgaaaagt
     7681 aaatagttga ttagtaaaca agtaaacgat ataaagaact ctacgaaaaa gaatacaaaa
     7741 gtagtcattt tttagttagt ttcaatatgt atttgataat gaaaactctg ccggagtttt
     7801 cgtagtccgg cagagtttat gactcttcag aatcagcctg catccttttt tcggggcgac
     7861 tgatgaacgg ttgttctaca tcagttccac gcttcttttc acaaatctgg ccagtgcttc
     7921 acccttcagc atgttgttgg ccagcaaagc cagatcaatg agttgtcgta cgtgtttgtt
     7981 gtcagcagcg tagtttctgt aaacggtttt tttgttttcg ttcagttcgt ctatcttttt
     8041 ttgcaggctg tccagttctt ctttttcgcc ggctggaatt tcttcgtctt ttttgccttt
     8101 ctgagtctct ttcaaactat ccagacgggt tttcatttcg cttatctgag ctttgagggg
     8161 tgtaacttcc gcttcagtgg cagtttcttc atctttcagc aatttttcta tcagcggatg
     8221 agccgtattc actacgagat tatagctgtc gggcatttcg ccatagaaac tcatcattcc
     8281 tccaccctgc atggccgaca tgtctttcat gcggcgcatg aattcatttt gagtcacgaa
     8341 aaccggatta gctgttgccg gaagttgttc gaaggtgaca atgaaattgg ttttgtccat
     8401 tgccggaatc tgactttcga agacagaaac aagggcattg cgttgatctt cgttcagcgt
     8461 tacttttcct gtctcttctt tttgaatcag tttgtcaatt acatcgctgt ccacccttac
     8521 aaaacgggtt ttctccgatt tctgctcaaa ctggctgatg gcatgtatgt ccagttgtcc
     8581 gtccatgatc agcacatcgt atcctttggc tctggcatct tcgatgtagg tgtactgctc
     8641 gtccttgttg gttgcgtaga gataaatgag gttgctgtcc ttatcggttt gctctgcggc
     8701 ggtaagttct ttgtattctt cgagtgtgaa atatttacca tccacatttt tcagcagtgc
     8761 gaatttttcg gcacgttcat agaatttttc gtcgctcagc attccgtact ggataaatag
     8821 cttcaggtcg tcccatttct gttcgaactg agttctgtcc gatttgaaaa tatcgttcag
     8881 ccggtcggca acctttttgg taatgtgtgc cgagattttc tttacgttgc tgtcgctttg
     8941 caggtagctg cgcgatacgt tgagcggaat atcgggtgag tcgatcacgc cgtgaagcaa
     9001 tgtgaggtat tcgggtacaa tgttttcaac atggtctgtc acgaaaacct gattgctgta
     9061 aagctggatt ttatttcgct gaatgtcgat attattcttt attttcggaa agtaaagaat
     9121 gccggtaaga ttgaacggat aatccacgtt catgtgtatg tggaaaagcg gttcgtccat
     9181 cgtcatcgga tacaaatcgt ggtagaatgt gctgtaatcc tcatctttca ggtcagcggg
     9241 ttttcgagtc caggcgggct tggtgttgtt tatgatgtta tcctcgtcgg tctccacttc
     9301 tttgccgtct ttccactctt ttttctttcc gaatgcgatt tctatcggca ggaatttaca
     9361 gtatttattc agcagttcgc gtatttttga ttcttccaga aaatccttgt tttcatcgtc
     9421 aatatgcagt acaatatcgc ttccgcgctc cgatttgatg gtctcttcga gcgtatattc
     9481 cgggtcgcct ttgcacgacc agtgctgtgc tacggctcct tccctgtaag actgggtgaa
     9541 tatctccacc ttttcggaaa ccatgaaagc cgagtaaaag ccaagtccga aatgtccgat
     9601 gatagcctga gcatcatttt tgtatttttc caaaaaatca cctgcactcg aaaatgcaat
     9661 ctggttgatg taactattta cctcatcggc tgtcatacca attccacggt cacttacggt
     9721 gagtgttttt ttctttttgt taatactgat tcgaatagtc agatcgccca attcgccttt
     9781 gtattcgccc acagcagcca gcgttttcag cttctgagtg gcgtcaacag catttgatac
     9841 cagttcacgg agaaaaattt cgtgatcgct gtataaaaac ttcttgataa cggggaaaat
     9901 attgtcactt gtaaccccaa tgtttccttt tgccatagta attttactta taaactcttg
     9961 attaagagat taataattta tttatttatt tcttatttgt ttgccgaaat taaattttca
    10021 caaaagttcc tgatactatt tttaaataaa cgattatctg atgtcgtttt tttaataccg
    10081 gttcttttgc taccgtattt tagcaaaata aatgccatta agtcacgata tgacaaaatg
    10141 gcagtcttta caaaattggt actcttttgt caggaaaccc ggtaaacatt tatgttaaaa
    10201 acttgttata aaaggacaat tataatacag caatggacaa aacaataaat acacatccga
    10261 aaggtacggc agaagggcag gcgctgctta tattcggagc taacggcgac ctctcaaaac
    10321 ggaaattatt gcctgccatt ttccagcttc acattgacgg actgctgccc gaaaaatttc
    10381 ttgtaattgg ctgtgggagc gacctgaaag atgaacatac attcaggaaa gatgtggagc
    10441 tgcacatgca tatttttgcg aaacagaatg tggaacggta tcctgagaaa ttcagttctt
    10501 tcattggaat gctttattac aagacagtga ataacaagca agccgaagat tttgaagaat
    10561 tgaaaaaata tgcatacgaa gtaaccgaca gaaatagtat tccgcataat ttcatttatt
    10621 acttttccat tccgccgttc atgtacgaga aagtgggaaa tcatctggtg agttgcggcc
    10681 tgaacagcga aaaggacgga tggaagcgga tcattgtgga gaaacccttt ggctacagcc
    10741 tcgattcggc agaagaactg gacagaaaac ttcacgaagg atttcaggaa catcagattt
    10801 atcggattga tcactatctt ggaaaggaaa ctgttcagaa tatcatggtg acccgatttg
    10861 caaacggttt tttcgaaccg gtgtggaatc gcagttacgt ggacagagtt gaaattacag
    10921 ctgccgagaa aatcggtgta ggcgatcggg gaggctatta tgaatcctcg ggagccttgc
    10981 gcgatatgat tcagaatcac ttgctgcagg tgcttgctat tgtggccatg gagccgcccg
    11041 ctgtttttga ttcggattcc attcgtaacg aaacggttaa agtgttacag gcattacgac
    11101 ccatcaagcc ggaagaagtg gaaaaatacg tggttcgagg tcagtactca gaaggaaaac
    11161 gacgtggaga aactatccct gcctatcgcg acgagaaaga tgtggttgcc gactcgacaa
    11221 ccgaaacttt tgtagccctg aaactctacg tggacaattg gcgatggagc gatgtgccat
    11281 tttatatccg aagcggaaaa aatctgcctg tacgcgttac tgaaattgta attcatctga
    11341 aacctgcacc tcttcagctt ttcaagcaac gatgcgccac tcagcagact aacatgatta
    11401 ttctccggat tcatccggat gccggtattg ccatcgattt cgggatgaaa atacccggtg
    11461 cgggctacaa agtgcagaat gtaaatatgg atttccatta ttcggatttg atgcaatcga
    11521 aaattcccga agcctacgag cgacttctgc tcgattgtat gacaggcgat tccacccttt
    11581 acgcccgtgc cgatgcggta catgcaagct ggaaattcgt ggattcgatt ctgaacacat
    11641 ggaacgaaaa tgctgaaata ccactgtatt tatatccggc caatacctgg ggaccaaaag
    11701 aagccgacgc acttttcggt gataagcgaa taaaatggca ggatcagtgt gataaatttt
    11761 catgtgaaat ttaaaataaa aataaagtac gatgctttac aacaaaaccc acataaatca
    11821 cgagcctgaa tcggtggccg aatcggttgc caggctcatt gtatcaaaag taaaacagaa
    11881 agaaggcaca ttggagcact tcaatctggc catttcgggc ggaaacaccc cgaatatact
    11941 tttcggattg ctggctgatg aatatgccgg acagattgac tggaatacac ttcggatttt
    12001 ttgggtggat gagcgttgtg tggaccctgc tgataaggaa agcaactacg gaaatgttca
    12061 taagattttg ttggaaaatg tacctttacc acccgaaaat atttttaata tccgcggaga
    12121 caacaatcct tcagacgaag tagtgcgtta ccgggaagtg cttaaaaaag aattgcccga
    12181 aaaaaacgga tttccggttt ttgacatgat actgctcggc atgggcgacg atggtcatac
    12241 tgcatccatt tttccggata acctggaatt gatccactct gcccaaaccg tagaagttgc
    12301 cttacatcct cacagcggac agtaccgcat taccctcacc ggcaatgtga tatgcaatgc
    12361 aaagcaagtg atttttttga ttaccggtaa ctccaaagcc gaaataattg aatctattct
    12421 gaacgaaaaa ggcgattttg agcggtttcc ggcctattat atcctctcgg gttgcgaagc
    12481 agagctttac ctggattttg aagctgcgga acggttgtag cggatggata agaaacgggt
    12541 aagaaatttt aaaaaataag ttggcaggga ttttgatgga tgaattttaa aaaacacaat
    12601 cacatgaaac gatttgcatt cattgcctca ttagttatgg tattctcggt agcttattcg
    12661 ggtgaaaaaa caaccattat cagggatata ctgactgaat atgcagctca aaaagtggat
    12721 aacaggcagg aattaattca attctccgat cagcaggcca aggagttgaa acaaacagaa
    12781 ctcaattact tactggaagt acagaaagct gaaaactgca ggtgttgcaa tactgcaaat
    12841 aaaattaaaa aatcagcaga aaaaagggac atcgaactcc aaaaaatcct tacacgagag
    12901 caatacctca aatacgaggc actggataaa aacaggataa aaaaacatcc gcttcgtgcg
    12961 ttgtaaaatc tataaaaaaa agaggcattt gcctcttttt ttatagttac atgtttcatt
    13021 tcagcatgtt tttcggaagt cggttatagt caagaattac tgcctctttt gctgcatcgt
    13081 ttggattact taatctcacc gaggcacttt cacttgcttt tattctgctt actggtatgt
    13141 tttgctttgg cttgtagctt tcgtcgccgg taatcaggta aatagcctgc cccaggaaag
    13201 cgtctttggt atcacctatc tgagtccagt tggatacata tccgttgcct tctgccagtg
    13261 cataagaagg tacaaggtat ccggggttcg aaaaatcttc gtttttgtag ttcgtatatt
    13321 tagccacaat gggttgcata gcccagtttt taagtttgtc cttttcatca gctttcagtg
    13381 aagcctcgtc gtagatattc accaccggat atttgtttat ttcctcttta tacggatgca
    13441 gggtccacga tgccgtgtat ttgccacctg tattcccgcc aatgtgtact acctgcatgt
    13501 aaggtttgag gcagaatgtg gtcagctccg atgccgaata tgagtcgctg gtggcaataa
    13561 tgtacacttt gtttaagttc agatttgcag ctaccggatc cggatcgggc ttttcataca
    13621 ctcccaggct gtcggtgcgg ctccatttgt ttttgtcgaa aattccgttc agataactgt
    13681 tgtaattcat cactatcaat ggcgatttgt ttctgacctt cgtctcgggt gcaatgagcg
    13741 aactcaggta aatggcagaa atgaccgaac ctccgtggtt gtaacgcagg tcaagcacca
    13801 ggtcggtgac gccggcctgc ctgaacgtgg agaaggcatc gtacaggctt tggttataat
    13861 ttctgataaa atcggtataa aacagataag caattttctt tccgccaata ttataaatat
    13921 ttgtgtagag taccggatcg gtccggatag aagccggtgt caacgtgatg gttttatcgg
    13981 ccaccagtcc ggatccggaa tttttatacg tagtaaaatt agcagtagcg ctaccgtaca
    14041 gaatcccgta attgctttcg gtaacgggct gtccgttaat tttacctatc atgtctttac
    14101 gctgcagacc tgcgttggaa gcaggcgagt taggaaatac atatttaatg atagctacgt
    14161 attcggtttt tgcctcattg gcgtaagcaa atgccagcga aaatccgaaa tcaacaggtt
    14221 cgcccgaaaa accggcaagc aaactttcca catcgtcggt tatgaatgac catccgtttt
    14281 cagtgtctgt tgcgttcagc acactcttga agtaggtttt cggatccaca tcacccgcag
    14341 ttggttcttt gcttttcatc tcgtcggccc agaggtaata agttgataat ccgtcatata
    14401 caaattgtga aaccggattt gccgttttcg gttcctcttt tttacacgca gagaatacca
    14461 gcagcaggct tgcgaaaagg actgaaagaa aaagaaattt tttcatttga aatgtgttgt
    14521 tgttttttaa aaaaaatgat catgtaaaag tatgaaacga gcatgaacac ggtggttcaa
    14581 caaagatgat gaaaatctga catgcgagct tgcctgcggt tggcaggttc catgcgacaa
    14641 ctaaaaaaca attatttacg catgaaataa atgcatcaaa aataataccc gaatcacaga
    14701 aattgagctg tatcagtcag ttttttttta ccggagatgc tatggcttaa tttcatcgag
    14761 caggtaagta cgatttaccc ggtatttgcc gttcacacgc tgcacggtgc aacattcgtt
    14821 gtaggcatcg tgttcgaaaa aaagaatctg ctgtttttca gcggcttcgc tcagcatcct
    14881 ttctttttcc tccatcgatg taatggggaa tgtatcgtat gccgataccc aggcgatggg
    14941 cagtgccgcc tgaaaaggga ttacatcgcc cgtgaaaacc acagttttag ctccaaaatg
    15001 gatgtaaggc accagctgac ccgcggtatg tccgttgaaa agccttacct tcacctccgg
    15061 acaaatgtac atatccttct caacgagttt caacctgccg gctttttcta ccggaatcat
    15121 gttttcggcg aagtaggagt cgccttcacg cacgttggga ttcagaaaat tttcccactg
    15181 cgcttttccc acccagtaag cggcgttcgg gaaggtgggg attatctctg atttgtcttc
    15241 gttgaaccgc gtactgctgc cgcaatggtc aaaatgaaga tgtgtatgaa taacatcggt
    15301 cacttcggcg gcagagtagc ccagtttctt cagttcggta tcaaaatcag caatatcttt
    15361 gaaatgatag tatttcagat attccagctg tttgactccg gtgccggtgt caatcacaat
    15421 cagtttttca tccgttttga ttaccagaca tcgcatcatg agcgtacaaa aattatcgtc
    15481 gttgcacgga tatcgttttt gccataccct tttgggtacc actccaaaca tggctccacc
    15541 atcggcaaga aacgaaccgg cttcaatttt atctaatttc atccttttta ttgagtcttt
    15601 tttggttaat tcttactaca tttgcacctt ataatggtta cttaacactt cattgaatcc
    15661 atttgttgcc agtcaaccat gcattttcac cgattcacaa atcggttcgt tatattaact
    15721 aatcgaattt gatacaaaaa tacaaaaaat taccgggtat gcaacgagtt cattttattg
    15781 ccattggagg tgcggccatg cacaatctgg ctattgcgct gagtaagaag gatgattatg
    15841 aaatcaccgg ttcggacgac gaaattttcg aaccttcgct cagcaggctg agaggacatg
    15901 gtctgctgcc caaagaaacc ggatggtttc ctgaaaaagt gaccaaccac ctgaatgccg
    15961 tcattctggg gatgcatgca agggctgata acccggaact gctgcgtgcc aaagagctgg
    16021 gaataaaaat ctactctttc ccggaatatc tctatcagca aacccgcagc aaaaccagaa
    16081 ttgtagtagg aggaagccac ggtaagacct ccacaaccgc catgattctg tatgtgatga
    16141 ataaactccg cttcaaggca gattatatgg tgggcgcaca gatcgagggt ttcgaaaata
    16201 tggtgcaact gtcatacgac tcgcgcgtgg cagtttttga aggtgatgaa tacctgacct
    16261 cgcccattga tccgcgacca aaattccacc tttacaaacc gcatattgct atccttaccg
    16321 gtattgcctg ggatcatatc aacgtttttc ctacttttga caactacgtg gatcagtttc
    16381 gcaaatttgt agatacgatg gaaatacagg gaaggctcat ctattacggc ggcgatgaaa
    16441 tcctgaatga catagcgcag aattgccggc aggatctcat cgtatttcct tataaaacac
    16501 caaaatacga aatcagaaac gggattacgt atgtaaaaac cggaaaagac gaggtggcgc
    16561 tgaaaatttt cggtgaacat aacctgcaaa atatggaagc agcccgtctg gcgtgccgtc
    16621 agataggcat tatggacgat aaattttacg aagctatctc cgacttcacg ggtgcttcaa
    16681 accgcctgca gaaaattgtg gaaaaacccg atcgggtggc tttcaaggat tttgcacact
    16741 caccatccaa gctcaaagct acagtaagag ctgtacgcaa acaatatccg gagcgcacgc
    16801 tcatagcctg catggaattg catacattca gcagcctgaa ttccgacttt ctgccgcagt
    16861 acgctgatag tatgaaagaa gccgatattg catacgtgta ttacaacccc gaagtcattc
    16921 atcataagcg gatggaagag ttggatccgg ataaagtgaa aagagcattt ggcggcgaaa
    16981 atttacttgt ttttaccgat actaacctgc ttcaggaaaa actgaaatcc ctgaattacg
    17041 aaaattcggc tctcctgctc atgagttcgg ggaatttttc aggtatcaat ttatctgaat
    17101 ttgccggaaa attgcttgcc acctgataca cggtagctga aattttgctg aatgctgtaa
    17161 tcaccggtcg tttggaaggt gtaaaacatt ttttttcttt ttaagaaaaa tattaataat
    17221 caatgaatct ttcattttct caacaggaga atagaaggac agacttttac atgatgaaca
    17281 aacaactttt actggacaaa cgttatatgc gcatggcccg aatctgggcc gaaaattcat
    17341 actgcgaacg gcgtaaagtc ggagcattgc tcgtgaaaaa caaaatgatc atttcagatg
    17401 gttacaacgg tacaccttca ggcttcgaaa acaagtgtga agacgataat aacgtttcga
    17461 aaccgtacgt gcttcatgcc gaagcaaatg ccatttcgaa agtagcccgg tcgcacaaca
    17521 gcagcgacgg agccaccctg tacgtcacag catcaccctg tatggagtgc tccaaactaa
    17581 tcattcaggc cggaatcaaa agggtggtgt acggcgaaac ataccgcatc atggatggtg
    17641 tggaattgct tcaacgggca ggcattgacg tagttttttt ggaaaatgtg gagtagattt
    17701 tttttagata ttttttgtaa gtaaattttt attgtaaatg aataaaagaa acatctggac
    17761 tattgtaatt atagttttct ctgctttggg cgggctttta cttggcaacg tgattgcaga
    17821 ccgcgcacaa aacggtaaga acctgagaga tttatcgggt ttattttcat ttggtaattc
    17881 atcatccaaa gtcaacgatt tattatcgct cattgacgga cagtatgtgg ataatgtaga
    17941 tatggacagc attaccgatg aagtgatggc tgatattgta tcaaaactcg atccgcattc
    18001 ggtgt
//
LOCUS       DAORKP010000031        17014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_218_length_17014_cov_8.291291, whole genome shotgun sequence.
ACCESSION   DAORKP010000031 DAORKP010000000
VERSION     DAORKP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218_length_17014_cov_8.291291"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..227)
                     /locus_tag="PLN75_03135"
     CDS             complement(69..227)
                     /locus_tag="PLN75_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03144.1"
                     /translation="MKRNLTFIIFAFILFAACQNEETVKMDKIPSNKLNGDFIVKDGR
                     IVFSELNS"
     gene            complement(372..749)
                     /locus_tag="PLN75_03140"
     CDS             complement(372..749)
                     /locus_tag="PLN75_03140"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3244 domain-containing protein"
                     /protein_id="HRG03145.1"
                     /translation="MKKTNLLVIIAVLILPFIMLAAQSTPNSKEIELMKETPSGPPRA
                     PAAPAINAYLSGDFITINYLSDLGNVHIEVKDESESIVYKCTEPSGANNTSYIDLYSF
                     ESGLYTITITNDGSLYLQGVFEL"
     gene            complement(913..2604)
                     /locus_tag="PLN75_03145"
     CDS             complement(913..2604)
                     /locus_tag="PLN75_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03146.1"
                     /translation="MRNTLLLLFVLSFTTILPTSCGREKSRADFIRMERLDSLLPEKP
                     GAVLDSLRMINPDFLTKYNRAYYHLLEITAKDKSYVDFSSDSLAKETAGTFKKSRNKY
                     PGLYSRSLLYLGIVRYRMGVIDSTAYIPLKEALDFLESNKEDNPAIQYFCSFYLGMIH
                     DKNDNPGESIDYFKEAIFFAEKTKDMRYLFTAKRDLFWAYMKAGEYEQAGESLDAIER
                     MPDLSELEVQDVKYAKAVHYNALGKNEESLKMNLSLYQDSRLRKLRASPSMLYRISKN
                     YSSLHQLDSALEYALGTVEHISDTADVLNYLYYYNAAEISEKLGHWRQSAEAFKTGAA
                     LRERAVEKELDTKIFELEKSYDKSKAENNALRYRNQNILLGLLSIILFMLLVFGLFAR
                     RQHMKHTSTKQLLEKREYQVLSQEKDRIEKKLIEKEFMLPLYKQISQRNAKITSLLTD
                     LSTNTYIVRNPELQQLISNACGDLIQSALINSEMFITDDKFMKFTNIDKRYNSQFNDS
                     EKMLLVFMSLELDNKQAAVLFNTSESSIRGRKSKLRRKLDGLNIDLAGISAADRQ"
     gene            complement(2875..10299)
                     /gene="sprA"
                     /locus_tag="PLN75_03150"
     CDS             complement(2875..10299)
                     /gene="sprA"
                     /locus_tag="PLN75_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRG03147.1"
                     /translation="MILRKKIISFIIFFLILVGTAYGFVSATRHFTSATAAELQQPQP
                     RKTTPAAVKDSATRFPVSNQAIETYEDLSKVYPMDLRNPENIKTEIEYDVNTGNYVIR
                     TKAGELEISTPMTLSPEEYNRYSLRKSMLEFYRDRNVKGVESNEDKFSATDMKFSLGP
                     ADKIFGPGGVQIKTQGSAELIFGFKSNFINNPVLPQRARRSNVPNFDQKIQMNVTGSV
                     GDKINFGLNYNTEASFDFDQKLAKLAFKGKEDDIIQNIEAGNVSMQLNGSLITGSTAL
                     FGIKTDLQFGKLRVSALASQQESESQTVSSKGGAQTIPFEVKIDQYDDNRHFFLGHVF
                     RDNYEQSMSKLPFISSGITVNRVEVWITNKRGNFNEARNIIGFMDLAEPRVIDNSIWK
                     NNSGAAVLANGSNSLYSEMNALPNVRDIQQTNAVLSATYPPASTGINGGEDYEKIENA
                     RRLDPSEYTVNNALGFISLRSALNLDEVLAVAYEYTYQGQIYQVGEFSTDNVKAPQAL
                     ILKLLKSTTQSPQLASWDLMMKNIYSIGAMQMQPEDFRMNIMYRNDSIGTELQYFPEG
                     NIASQLLLRVMNLDRLDVKNTVNPDGLFDYVEGYTALSSSGRIIFPVLEPFGSHLRAK
                     IGNDAIADKYVYQELYDSTLVVAQEFSEKNKFSLVGRYKGSASGSEIRLNAMNVPRGS
                     VVVTAGGKTLVENVDYTVDYMMGTVTILNQSIIESNTNVNVKLENQSMFSMQRKSLLG
                     THLEYQFSKDFSLGGTLMHLSEMPLTKKVNTGSEPIKNTIWGLNTSWRRESQWITNVL
                     DKFPFVNATQPSSIAFNAEYAQLIPGHSKVISEMGLAYLDDFESTKTNIDIHYPYNWY
                     LASTPGMFDESKLSNNTDYGKNRALLAWYAVDRILNSEARTTPPNLRGNLESQSNHYT
                     RQVRETELFPNRDIDPTGTSLMTLLNLSFYPEERGPYNLDIAGVNPATGKLLNPKDRW
                     GGIMRKIETSDFETANIEYIEFWMMDPFVYGTQNNSGGDLYFNLGDISEDILKDGKKA
                     FEHGLPIDNTNQNFETTVWGRIPLTQSTVIAFDNTAGAREKQDVGLNGLSTQDEFTFP
                     TYKTYVENLTAHLDPAVVTRMQNDPHSPINDPSGDNYHFYLGKDYDERNLSILERYKY
                     YNGTERNSPDAAETGTEYSTSATLAPDIEDINSDNTLNEYEKYFQYKVQLRPDNMMVG
                     TNFITDLVEASVTLKNGKTENVKWYQFKIPIREYTDKVGSIRNFKSIRFIRMFLSGFE
                     KEAHLRFASLDLVRGEWKTYTKPLAVSASASNARLDVLAVNIEENGRKTPVNYILPPG
                     ITRETDPSQPQLLQQNEQAMVLRVSALEPDDARAVYKKTSNDMRQYKRFQMFVHAEAM
                     KAAPDLNDDDLAVFVRMGSDMVNNYYEYEIPLRLTPEGTYTSNKLGDRQTVWYPENMF
                     DFPFTVLTDTKLQRNRERGGSVSNALPFSVFDEKNPKNRITVMGNPSISEVENIMIGV
                     RNKSGAVKSGEIWVNEMRMSEFEEDGGWAALGNLAVNLSDLGSLNVAGRMETAGFGGI
                     ESNVTNRNMEDHYSVNLSASLDLGRFLPEQVKLQIPAYYTYTTESSSPKYNPLDEDIL
                     LKDAIGNLESQSLKDSLKALTQSVIRTRSFNITNAKVNIRSKKPMFYDPANVTVSYST
                     NETNEHTPEIEQNLTKQQRAAINYTYSFNMKPWKPFEKTKTLAKPAFKLIKDFNLYYL
                     PASVNYSSSLNRAYSQMKLRDFTGFNTGSVLDNLSFSKDFMWNRQFDFKYKITEAIDL
                     GLQTSMNANIEESYFTPEIGKEYYEAWRDTVWSNIRKMGTPYTYQQIFNASWKIPIDK
                     LPLMDWVTANAQYNSTYSWNRSAIMEGGTDVGNLASSMGRLTANADMNLESLYKKSKF
                     LKAVIERGQRNTRQQTFNPRTFNKNVNLKKGDTLTVNHALNSERFTFTAKDSLGRPIR
                     VPYKVKNASSVLLIPVMNASNATVTIISKDPNERTITEKLADFTIRLPLTIRKVSVNY
                     NQSSSLTIPGFLPEPGFLGQKKLNGIPTPGWAFVFGYHDENTLSGASERGWLYMGEDI
                     INPANEALTSDLDIRATMEPIAGLKIDLTGKRQFQSNTVIHYMYPGMPSTFNGNFNIT
                     QVALKTAFDKIGNASDNYRSATFSRFLANRELIARKLESRYTGTRYPVTGFFADNPVY
                     RGQPFDPENGTFDINSPDVLIPAFLAAYTGRDVNRINTNPFLSILEIMPNWNINFNGL
                     SNIPWVKDKFRSISITHGYSNRYTIGNYSSFSTWVSQDGNNSALGYIRDVQLNMPIPS
                     MAYDISTVSLNESFSPLFGLNLAMKNSMTTKAEYRKQRNLALNLNSTQLIEALSDEFV
                     VGVGYVVNDFDVVLKLKSSKRQTVKNDLKMNLDFSYKDIKSLLRKVSEDITQASSGNK
                     VMSLRFTADYVFSSKVNLQLFFDRQMTAPLVSSSYPVSSTNFGLSFKFMLTR"
     gene            complement(10365..10964)
                     /gene="ruvA"
                     /locus_tag="PLN75_03155"
     CDS             complement(10365..10964)
                     /gene="ruvA"
                     /locus_tag="PLN75_03155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRG03148.1"
                     /translation="MIDYIKGAVTELTPTGVVLETAGVGYYLNITLTTYSALNGREDA
                     QLFVYEAIREDAHLLFGFMNRAERQLFLLLISVSGIGANTARVIMSSYSASEIQQMIA
                     GGDVVSLNAIKGIGTKTAQRIIVDLKDKILKTGIAEESGLKGTMHDPKTNSVREETLA
                     ALAMLGFAAAASQKVVDKIMAENPTMKVEQVIKQALKML"
     gene            complement(11345..12049)
                     /locus_tag="PLN75_03160"
     CDS             complement(11345..12049)
                     /locus_tag="PLN75_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRG03149.1"
                     /translation="MNSIRLTVLIAALLFAAVSTNVHGQDSLQVLPIVEDTLRLPLQE
                     LTDSNKHFNQQFNFENRSFGNKFLRGTGYSFLFSLSIGMYLVVAPPEITQWYAENKFK
                     LPVMQKQYRKTFTTPPVIDHDLWVVNYIGHPYQGSYYYNTIRSQGGTLLQSSLHNLFQ
                     SLFWEYVWEGGVEQPSIQDIIVTPIAGSLVGELTHKATLSMRKNGFRWYEKVATCLIN
                     PAYVLNNGFSLTKRRK"
     gene            12315..13943
                     /gene="ade"
                     /locus_tag="PLN75_03165"
     CDS             12315..13943
                     /gene="ade"
                     /locus_tag="PLN75_03165"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRG03150.1"
                     /translation="MNTIAGHVVDVVARQIRKGTLYIDGGKIVNFEASDAVPDQYIIP
                     GFVDAHIHIESSMMLPSEFARYSLPCGTVACVCDPHEIANVCGIPGVNYMIENGKKSA
                     LKYYFGAPSCVPATPFDSSGAILDAEAVSKLLERDDIYFLAEMMNYPGVIEQDEQVHL
                     KLRAAHKHLKPIDGHAPELTGEGLIAYASAGITTDHECMTIAEAEAKIALGMKVLIRE
                     GSAAKNFDDLLPLLEKYSDEIMFCSDDKHPDDLIKNGHINTLARRAVAAGYDALEVLR
                     ICSLNPVLHYKLDVGLLQQGDDADFLVVNNLTDFDVKQTYIRGLKVAENNAVLFPRYI
                     PNKLINNFKATPLQPEQLRVQPSGDQLKVIVVDDGQLFTHSELVVPKIDNDNVISDTT
                     NDILKLVVYNRYQDMKPAIGFIKNIGLKEGALASTVSHDSHNIVAVGTSDEEIVSAIN
                     HIIASTGGILACNKDNVLLLPLPVGGLMSAEEGDVIAQKYEELDAAVKQMGSQLKAPF
                     MTVAFMSLLVIPKLKLSDQGLFDGRTYAFTDLMI"
     gene            14397..14534
                     /locus_tag="PLN75_03170"
     CDS             14397..14534
                     /locus_tag="PLN75_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03151.1"
                     /translation="MRQVTEAGVATIEIPVINTDLYLYNFRSLPSFTIVACLGGVCDM
                     T"
     gene            14586..14798
                     /locus_tag="PLN75_03175"
     CDS             14586..14798
                     /locus_tag="PLN75_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03152.1"
                     /translation="MRGISQSDWMPATGTQPGFSIRLYCPLKLMPITDDVTMLVCLGV
                     EFGKNGYGNTTEPVKYAGTEKIVRVG"
     gene            complement(14926..15345)
                     /locus_tag="PLN75_03180"
     CDS             complement(14926..15345)
                     /locus_tag="PLN75_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRG03153.1"
                     /translation="MKHVITSEWKGNLTFESTIDEHKIINDAPLAAGGDNLGTSPKKL
                     LLVALAGCTGMDVVSILKKMRVNIAKCNISVEADMRDEHPKYYERMHVVYEFTGKDLP
                     MEKLEKAVKMSEETYCGVEALFRKAIEVTSEIRVKEG"
     gene            15702..16610
                     /locus_tag="PLN75_03185"
     CDS             15702..16610
                     /locus_tag="PLN75_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRG03154.1"
                     /translation="MHPIASFQELTSHLTESQHRKRMAVANAVDSHTLDAVINAVNAG
                     IVEAYLVGDVAEIESYLLEQPDISSYIHIIDKPDAGEATLEAVRMVKDGECDILMKGL
                     VNTDVILRAILDKQKGLLPPGNVLTYNAALEIPGYHKLIFFSDPAVIPYPTLDQRKAM
                     IKYAINTCYKFGLSKPKVALIHATEVASPKITFMQDYLDIMQMWRAGEFGDVIIDGPL
                     DIFLALDAERGGIKKVPSPLLGDADVLIFPDFAAANVFYKGVALFANAQMGGILQGTS
                     KPCVLTSRSETALSKFYSIAVACILA"
BASE COUNT         4450 a         3707 c         3808 g         5049 t
ORIGIN      
        1 taatacaaag agcaaggtta taaataccaa acttagtttt ttcatgagtt tattttctat
       61 ttttatattt aagaatttaa ttcgctgaat actattcgtc cgtcttttac aataaaatcc
      121 ccatttaatt tattgctagg gattttgtcc attttaactg tctcttcatt ctggcaagct
      181 gcaaataaaa tgaatgcaaa aataataaat gttagatttc ttttcatact ttttgttttt
      241 aaatgttaga aatgaaatta atatatgaga actttcttta gttttagttt tcaaacacat
      301 aattaattta ggccatttta ttggactttt aaacatatgt tatagatgtt gatacactat
      361 gaagatccgt gttataattc aaatacgcct tgaagataca gacttccatc attagtaata
      421 gtaatggtat acagaccact ttcaaaggaa tataagtcaa tgtaacttgt attattagca
      481 ccagatggct ctgtacactt ataaactata gattctgatt catctttaac ctcaatgtgt
      541 acatttccca agtcggataa ataattaatc gtaatgaaat ccccagatag ataggcattt
      601 attgcaggag ctgctggtgc gcggggaggt ccgctgggtg tttctttcat taactctatt
      661 tctttactgt taggcgttga ctgagctgcc agcataatga aagggaggat taataccgca
      721 ataattacga gaagatttgt ttttttcata cggatgtgta gttttggata tattgatatg
      781 caaagctatc agatataaat cagctgcaca aatgttttaa ggtgaggagt gagaagtagc
      841 gctgaaaaac aacgtatatc actgtgagac tgttttttac aattttaaag taacgcgcag
      901 aaagcatttt atctattgcc tgtcagcagc gctgatgcca gccaaatcaa tattcagccc
      961 atccagtttt cttctcagtt ttgattttct tccgcggatg ctcgattctg aggtgttaaa
     1021 cagcacagcg gcttgtttat tatccagctc aagtgacatg aataccagca gcattttttc
     1081 actgtcgtta aattggctgt tatacctttt gtctatattg gtgaatttca taaacttgtc
     1141 atccgtaata aacatttccg aattgataag ggcgctttgg attaaatctc cgcatgcatt
     1201 ggaaataagc tgttgtagtt ccgggtttct gacgatgtag gtatttgtcg aaaggtcggt
     1261 tagcagggat gtgatttttg cattccgttg tgaaatctgc ttgtataaag gcagcatgaa
     1321 ttctttctcg ataagttttt tttctatcct atccttctcc tggcttagca cctgatactc
     1381 tcttttttcc aaaagctgtt ttgttgaggt atgcttcata tgctggcgcc gggcgaacaa
     1441 tccgaaaaca agcagcataa ataaaatgat gctcagaagt ccaagcaaga tattctggtt
     1501 tctataccga agggcgttat tctcagcttt tgatttgtcg tatgattttt ccagttcaaa
     1561 tatcttcgta tcaagctcct tttctactgc tctttctctt agcgcagcac ccgttttgaa
     1621 tgcttcggcg ctctgcctcc agtgtccgag tttttcagaa atttcagccg cattgtaata
     1681 gtagagataa ttgagaacat ctgccgtgtc gctgatgtgc tccacagttc ccaaggcata
     1741 ttcaagagca ctgtcaagtt gatgaaggct gctgtagttt tttgaaatcc tgtacagcat
     1801 gcttggggat gccctcagct tcctgagtct tgaatcttga taaaggctta gattcatttt
     1861 cagagattcc tcattttttc ccaatgcatt atagtgaacc gctttagcat atttgacatc
     1921 ctgcacttca agctctgata aatcgggcat cctttcgatt gcatccaggc tttctccagc
     1981 ctgttcgtat tcacctgctt tcatataagc ccaaaataaa tcccgtttcg cagtaaaaag
     2041 atagcgcata tcctttgtct tttcagcaaa aaatattgct tccttgaaat aatcgatact
     2101 ttctccggga ttatcatttt tatcatgaat cattcccaaa taaaagctgc agaagtattg
     2161 aattgcaggg ttatcctctt tattgctctc caagaaatcc aacgcctcct tcaatggaat
     2221 gtaggctgtg ctgtcaatca cacccattcg gtaacggact atcccgagat acagcagcga
     2281 acgggaataa agtcccggat acttgtttct actctttttg aatgttcccg cggtttcttt
     2341 ggcgaggctg tcggatgaaa aatcaacgta cgatttatcc ttagccgtaa tctcaaggag
     2401 atggtaatat gcccggttgt attttgtcag aaaatcgggg ttgatcattc gcaggctgtc
     2461 caaaactgct ccgggtttct ccggcagcag gctgtccaat ctttccatgc ggataaaatc
     2521 agctctgctt ttctccctgc cgcatgaggt aggcaggatg gtggtaaagc ttaatacgaa
     2581 caacagaaga agggtgtttc tcatagtaaa aatcggatga atcagatatt cacatcataa
     2641 aaatacgtaa aaatatcagg ttgccatctt attgcaaaga tacgtattgt ttttaattga
     2701 agtcttctcg actttaaaac atagaaaaca agagacttat tttattaaat ttttagtgtt
     2761 gctttaattg aattcgataa cattagtatt actattttta ttaatttttc agaataatta
     2821 caagtaatga tgtaccttgt atattcatcc ggagtagatt cgtaccgtaa attgctacct
     2881 cgtgagcata aacttgaaac tcagtccgaa attggtggaa gaaaccgggt aggacgatga
     2941 tacgagcggg gcggtcattt gccggtcaaa gaacagttgc agattcactt tcgaactgaa
     3001 cacgtaatcg gccgtgaaac gcagcgacat cactttattg ccgctggaag cctgcgtgat
     3061 gtcttcactc acttttcgca acagcgattt gatatcctta tatgaaaaat ccagattcat
     3121 tttcaggtcg tttttcactg tctggcgctt actcgatttg agtttcagca ccacatcgaa
     3181 atcgttgacc acatagccca ctcccaccac aaactcgtcg gacagggctt cgataagctg
     3241 cgtgctgttc agattaagcg ccagattacg ctgtttgcgg tattcggctt tggtagtcat
     3301 gctgtttttc atggcaagat tcaatccgaa aagcgggcta aaactctcgt tgagtgaaac
     3361 ggtggaaata tcatacgcca tggacggaat tggcatattg agctgaacat cacgtatata
     3421 acccaaagcg ctgttgtttc catcctgaga tacccacgtg gaaaacgagg agtaattgcc
     3481 aatggtgtac cggttggaat aaccatgagt aatgctgatg gaacggaact tatctttcac
     3541 ccaggggata ttggatagac cgttgaaatt tatgttccag ttaggcataa tctcgaggat
     3601 ggaaaggaaa gggttggtgt tgatccggtt cacatccctg ccggtgtaag cagccagaaa
     3661 cgccgggata agcacatccg gtgaattgat atcgaaagtt ccgttttcag gatcgaaagg
     3721 ctggccccgg taaacgggat tgtcggcaaa aaatccggtg accggatacc gcgtgccggt
     3781 atatctgctt tcgagtttac gggctatcag ctccctgttg gccagaaaac ggctgaatgt
     3841 ggctgaccgg tagttgtcag atgcgtttcc tattttgtca aatgccgttt tcagagctac
     3901 ctgcgtgatg ttgaagttac cgttgaaggt ggagggcata ccgggataca tgtagtgaat
     3961 cactgtgttt gactgaaact gtcttttgcc ggtcaggtca attttcagtc cggcaatggg
     4021 ttccatcgtt gcacggatat ccagatcgga agtcagagct tcgttggccg gattgataat
     4081 atcttcgccc atgtagagcc atccgcgctc agaggcgccc gaaagggtgt tttcatcgtg
     4141 ataaccgaac acaaacgccc atcccggtgt cggtattccg ttcagttttt tctgtcccag
     4201 aaaacccggt tcggggagga atcccggaat ggtgagactg ctcgactgat tgtagttaac
     4261 tgatactttg cgtatagtga gcggcaggcg tatggtgaag tcggccagtt tttctgttat
     4321 cgtgcgctcg ttcggatctt tgctgatgat ggtgacggtg gcattggagg cgttcatcac
     4381 cggtatgagc aacacggaag aagcgttttt caccttgtaa ggtacgcgta taggccttcc
     4441 cagcgaatct ttggctgtga atgtaaatct ttcgctgttc agggcatgat ttacggtaag
     4501 tgtatctccc tttttgaggt ttacattttt attgaaagtc cgcggattga acgtttgctg
     4561 gcgggtgttg cgctgccccc gttcgattac cgccttgaga aattttgatt ttttatacag
     4621 actttccaga ttcatatcgg cattggcggt aagcctgccc atggatgaag ccagatttcc
     4681 cacatcggta cctccttcca tgatggcgct ccggttccag ctgtaggtgg agttgtactg
     4741 cgcattggcg gttacccagt ccatcagtgg gagcttgtcg attggaattt tccacgatgc
     4801 attgaaaatc tgctgatagg tgtacggtgt gcccattttc cggatgttgc tccataccgt
     4861 atcgcgccat gcttcgtagt attcttttcc aatttcgggt gtgaagtagc tttcttcgat
     4921 gttggcattc atggaagtct gcaaccccag atcgatggct tcggttattt tatatttgaa
     4981 atcgaactgc cggttccaca tgaaatcctt gctgaaactc agattgtcca acaccgatcc
     5041 ggtgttgaat ccggtgaagt cccgcaattt catctgcgag taagcacggt tgaggctgct
     5101 gctgtaattt acagatgcag gcaaataata caggttaaag tctttgataa gcttaaatgc
     5161 cggcttggcc agtgttttcg ttttttcaaa aggtttccac ggcttcatat tgaaactgta
     5221 ggtgtagttg atggcagccc gctgctgttt ggtaaggttc tgctctattt cgggcgtgtg
     5281 ttcgttcgtt tcgttggtcg agtagcttac tgttacgttt gcagggtcgt agaacattgg
     5341 ttttttactc ctgatgttga cttttgcatt cgttatattg aaacttctgg ttcgtatcac
     5401 cgactgggtg agcgctttca gcgagtcttt gaggctttgc gattccaggt taccgattgc
     5461 atctttcagc aggatgtctt cgtccagcgg attgtatttg ggcgaacttg attcggtagt
     5521 ataggtgtag taagccggaa tctggagctt gacctgttcg ggcagaaaac gacccagatc
     5581 gagtgatgcc gagagattga cggaataatg atcttccatg tttctgttgg tcacattgct
     5641 ttctattcca ccaaagccag ctgtttccat tcttccggcc acattcagcg aaccgagatc
     5701 cgacaagttg acggccagat tgcccagtgc tgcccagcca ccgtcttctt caaattcgga
     5761 catcctcatt tcattcaccc atatttcacc cgattttacc gccccgcttt tattgcgaac
     5821 cccgatcatg atattttcca cctccgaaat ggaagggtta cccatgacgg taatccggtt
     5881 tttcggattt ttttcgtcga aaacactgaa aggaagcgca tttgaaaccg atccgccccg
     5941 ttcgcggtta cgctgaagtt tggtgtcggt gagcacagtg aacggaaaat caaacatatt
     6001 ttccggatac cataccgttt gtctgtctcc cagtttattg ctggtgtaag tgccttcagg
     6061 tgtgagtcga agcggtattt cgtactcata gtaattattc accatgtccg accccattct
     6121 cacaaacacg gccagatcgt cgtcattcag atcgggagct gccttcatgg cttcagcgtg
     6181 tacaaacatc tggaatcgct tgtactggcg catgtcgttg gaggtttttt tgtaaacggc
     6241 acgtgcatcg tcgggttcga gtgcggatac ccgaagcacc atggcctgct cattttgctg
     6301 caggagctga ggctggctcg ggtcggtctc acgggtaatt ccgggaggga gtatgtaatt
     6361 aaccggtgtt ttacgcccgt tttcttcaat gtttaccgcc agtacatcca ggcgggcatt
     6421 ggaggcagaa gccgacacag caagcggttt ggtgtaggtt ttccattcac cgcgcaccag
     6481 atccagggag gcgaaacgga gatgtgcctc tttttcgaat cccgaaagga acatacgtat
     6541 aaaccgaatg gatttgaaat tgcggatact gcccactttg tcggtatatt cccgaatggg
     6601 tatcttgaac tgataccatt tcacattttc cgtttttccg tttttcagcg ttacgcttgc
     6661 ttccaccagg tcggtgatga aattagtacc taccatcatg ttgtccggac gaagctgcac
     6721 cttgtactgg aaatattttt cgtactcatt cagggtatta tccgaattta tatcctcaat
     6781 atccggcgca agtgttgccg aagtgctgta ttcggttccg gtttcggcag catcgggaga
     6841 atttctttcg gttccgttgt aatacttgta ccgttcgagg atgctcaggt tccgttcgtc
     6901 ataatctttg cccaggtaga aatgatagtt gtcgcccgaa ggatcgttga tgggcgaatg
     6961 cgggtcgttt tgcatccggg tcaccactgc gggatccaga tgagctgtga gattttccac
     7021 ataggttttg taagtcggga aagtgaactc atcctgcgtg gaaagcccgt tgagccctac
     7081 atcctgtttt tcgcgtgcac cggccgtatt gtcaaaggca ataacggtgg actgagtaag
     7141 cggaatgcgt ccccaaacgg tagtttcgaa attctgattc gtattgtcga tgggcagtcc
     7201 gtgttcgaac gctttttttc cgtctttcag aatatcttcg ctgatgtcgc ccagattgaa
     7261 ataaagatca ccgcccgaat tattctgagt tccataaaca aacggatcca tcatccagaa
     7321 ttcgatgtat tcgatgttgg cggtttcgaa gtccgacgtt tcgatcttcc gcataattcc
     7381 tccccaccga tccttcgggt tcagcagctt tccggttgcc ggattaacac ctgctatgtc
     7441 caggttgtaa ggtccgcgct cttcggggta aaaggacagg ttgagcagtg tcatcagcga
     7501 agttcccgtc ggatcaatgt cgcgattggg aaaaagttcg gtttcgcgca cctgacgggt
     7561 ataatggttt gattgcgatt cgagattgcc ccgcaggttg ggcggtgtgg tacgcgcttc
     7621 gctgttcaga atcctgtcca cggcatacca tgcgagcaaa gcacggtttt taccgtaatc
     7681 tgtgttattg cttagttttg attcatcaaa cattcccggc gtactggcca ggtaccagtt
     7741 atacgggtaa tgaatatcta tgttcgtttt tgtcgattca aaatcatcga gataggccag
     7801 ccccatttcg ctaatcactt tggagtgtcc gggaataagc tgagcatatt cggcattgaa
     7861 ggcaatggac gaaggttgtg tagcgttcac gaaaggaaat ttatccagca cgttggttat
     7921 ccattgcgat tcacgtctcc aggaggtgtt gagtccccaa atcgtatttt tgatgggttc
     7981 acttccggtg ttcacctttt tagtcagcgg catttcggag aggtgcatca gcgtaccccc
     8041 gaggctgaaa tccttgctga actgatactc caggtgtgtt cccagcagcg atttgcgctg
     8101 catgctgaac atcgactgat tttccagctt cacatttaca tttgtattgg attcgatgat
     8161 agactgattg agtatggtga cagttcccat catgtaatcc accgtgtagt ccacattttc
     8221 tacaagcgtt ttacctccgg cagtcaccac caccgatcct cgcggtacat tcatggcatt
     8281 caggcggatt tccgaaccgg aagcagagcc tttgtacctc cctaccagcg agaatttgtt
     8341 tttttcgctg aattcctgag ccactaccag cgtggagtcg tacaactcct gatacacgta
     8401 tttatcagct atggcatcgt tgcctatttt ggcgcgcagg tggctcccga aaggttcgag
     8461 taccggaaaa atgattcttc cggacgaaga gagcgccgtg tatccttcca cataatcaaa
     8521 gagcccgtcg gggttgacgg tattcttcac atccagcctg tccaggttca tcacccgcag
     8581 caaaagctgg ctggcaatgt ttccttcggg aaaatactgt aattcggtgc cgatagaatc
     8641 attgcggtac atgatattca ttctgaaatc ttccggctgc atctgcatgg ctccgatgga
     8701 gtaaatattc ttcatcatca ggtcccatga agccagctga ggcgactgtg tggtgctttt
     8761 caacaattta agaatcaacg cttgcggtgc tttcacgttg tcggtggaga attctcccac
     8821 ctgataaatc tgcccctggt aggtgtattc gtaggccact gcgagcacct catcgagatt
     8881 gagggctgaa cggagcgaga taaaacccag cgcattgttg accgtatatt ccgatggatc
     8941 gagccggcgg gcattctcaa ttttttcgta gtcttcgccg ccgttgatgc ctgtggaagc
     9001 cggcgggtaa gttgccgaca aaacggcatt ggtctgctgg atatcgcgca cgttgggcag
     9061 ggcgttcatt tcgctgtaca acgagtttga gccgttggca agcacggcgg cacccgaatt
     9121 gtttttccaa attgaattat caatgacacg cggttcggcc aggtccatga aaccgatgat
     9181 gttgcgggct tcgttgaaat ttccgcgctt gttggttatc cacacttcca cacgattgac
     9241 agtgatgccg gatgaaataa acggcagttt actcatggac tgctcataat tatcacggaa
     9301 aacatgaccg agaaaaaagt ggcggttatc gtcgtactgg tcaattttga cttcgaaagg
     9361 gatggtctgt gcgcctcctt tggaacttac ggtctgcgat tcggactcct gctgggaggc
     9421 aagcgccgaa acgcgcaact tgccaaactg cagatcggtt ttgataccga aaagcgctgt
     9481 gcttccggta atgagcgaac cgttgagctg catgctcaca tttccggctt cgatattctg
     9541 gattatatca tcttccttgc ccttgaaagc cagtttagcc aacttttggt cgaagtcaaa
     9601 agaggcttcg gtgttgtaat tcagtccgaa attgatttta tcgcccaccg atccggttac
     9661 gttcatctga attttctgat cgaagttcgg tacgttggag cggcgtgcac gctgcggaag
     9721 aacaggattg ttgataaaat tacttttgaa accgaaaatg agttctgccg atccctgtgt
     9781 tttgatctgc acaccacccg gtccaaaaat tttatcggca ggcccgaggc tgaatttcat
     9841 atcggtcgct gagaatttat cttcattgct ttccacaccc ttcacgttgc ggtctctgta
     9901 aaactcgagc atgcttttac ggagtgaata cctgttgtac tcttcgggac tgagggtcat
     9961 gggagtagat atttcgagtt cgcctgcttt ggtgcgaatg acatagtttc cggtatttac
    10021 atcgtactct atttctgttt tgatgttttc gggattccgg agatccatgg ggtaaacctt
    10081 gctgaggtct tcgtaggtct cgatggcctg gttgctcacc ggaaatcggg tggcggaatc
    10141 ttttacagct gcaggagtgg ttttacgtgg ttgcggctgc tgcagttctg ctgcagtggc
    10201 ggacgtgaag tgtcgggtag cacttacaaa gccgtaagca gtaccaacca aaattagaaa
    10261 gaagatgatg aaagaaatta tttttttcct taaaatcata tcgttatctg ttaccgcacc
    10321 ttatgtcggc tgcaactctg ctaatcgttt tttattctgt ccggttacag cattttcaat
    10381 gcctgcttta ttacctgttc cactttcatc gtcggatttt cagccataat cttatccacc
    10441 actttctgcg aggcggcagc tgcaaagcca agcatggcaa gtgcggcgag cgtttcttct
    10501 cttactgaat tcgttttcgg atcgtgcatg gttcccttca gtcctgattc ttcggcaatt
    10561 cctgttttca gaattttatc cttcaggtct acaataatgc gctgggcggt tttggtaccg
    10621 attcctttga tggcattcag cgatactaca tcacctccgg ctatcatctg ctggatttca
    10681 gatgcactgt acgacgacat aatcaccctg gctgtattgg cgccaatacc cgaaacggat
    10741 atgagcagca ggaaaagttg tctttcggca cggttcataa atccgaacag gaggtgtgcg
    10801 tcttcgcgaa tggcctcgta gacaaaaagc tgagcatctt ccctgccgtt aagtgcgctg
    10861 tatgttgtga gtgtaatgtt tagataataa cctacacctg ccgtttcgag cacaacaccg
    10921 gtgggggtga gttcggttac agctcccttt atatagtcaa tcattgttct aacaaatttg
    10981 agtttatatc atttatgaaa acggaattta caaccgcaaa atcatttggt tgcattcgga
    11041 aagtataacg attaattaag agaaatagta caatttatgc ctaaaaagac gacacaatgt
    11101 gcatgagaag cgagtaatac agcgcaatac aatcagttga aggctgagag caagcagcgg
    11161 agaaggattt ccaaaaatac gtttagtaat cagtgaattg aaaagaacag caaacagaaa
    11221 acattttcag cgtaaatctg ccattttttt tattgattga aaaaacattt tctgagatac
    11281 ttctccccta gtagttgaac cgttaattat agggtgaatt ggtcgaagtc attctttggg
    11341 aatcttattt tcttcgtttt gtaagactga atccgttatt gaggacataa gccggattga
    11401 taagacacgt agccactttt tcataccatc ggaacccgtt tttacgcatg gacaatgtcg
    11461 ctttgtgggt gagctccccc accaaagagc ccgcaatggg tgtcactatt atatcctgaa
    11521 tgcttggctg ctctacaccg ccttcccaaa catactccca aaacagtgac tgaaacaagt
    11581 tatgcaatga agattgcaac aacgtgccgc cctgcgatcg gatggtattg tagtaatatg
    11641 agccctgata gggatgtccg atgtaattga ccacccacaa atcatgatcg atgacaggag
    11701 gtgtggtaaa cgtttttcga tactgtttct gcatgacggg taacttgaat ttattctccg
    11761 cgtaccattg ggtaatttca ggaggagcta caaccagata catcccgata gacaaactga
    11821 agagaaatga ataacccgta ccacgcaaaa atttattacc gaaagaacga ttctcaaaat
    11881 tgaattgttg attaaaatgt ttgtttgagt cggtgagttc ctgcaagggc agtcgcagcg
    11941 tatcttcaac gatgggaaga acctgaaggg agtcctgccc gtgtacattt gttgatactg
    12001 cagcaaacag cagtgcagca attaacaccg tcagcctgat tgaattcata cccggtttta
    12061 ttttcatttt gtatcatcat gaaacatatt gcaataataa tcaattattt tgaaagtgaa
    12121 agctaaaatg agataaaata attcctttcc ggttttctaa acttgtattt gaaacaagcg
    12181 acatccaggt agattgaaga taagcaggag ttttttttat ttactaatct ttctgaacaa
    12241 aaaccttatt tttatcctta tatacaaaca tttaagaata aaaacaatta aaaaaacttt
    12301 ataaaacctg aaaaatgaat acaatagcag gacacgtagt agatgtagta gcgcggcaaa
    12361 taagaaaagg aactctatac atcgatggag gaaaaattgt aaactttgaa gcctcagatg
    12421 ctgtgcccga ccagtacatt attcccggtt ttgtggatgc acacatccac atcgaaagct
    12481 ccatgatgtt gccatcggag tttgcacgct acagtttgcc atgtggcacc gtcgcctgtg
    12541 tttgcgatcc acacgaaata gccaatgtat gtggtatacc cggtgtaaat tacatgatag
    12601 aaaatggcaa aaaatcggca ctcaaatact actttggtgc tccttcgtgc gtgcctgcta
    12661 cacctttcga ttcttccgga gccatcctcg atgccgaagc agtgagtaag ttgctcgaac
    12721 gcgacgacat ttacttcctt gccgaaatga tgaactatcc gggagttatc gagcaagacg
    12781 aacaggtaca tttaaaactc cgggctgccc acaaacatct caaacccatc gatggacatg
    12841 cacctgagct taccggcgaa ggcctgatag cttatgcttc ggctggtatc acaaccgatc
    12901 acgaatgcat gacaattgcc gaagctgaag ctaaaatcgc ccttggcatg aaagtactga
    12961 tccgcgaagg tagcgctgcc aaaaacttcg acgacttact acctctgctc gaaaagtaca
    13021 gcgacgaaat catgttctgt tccgacgata agcaccctga tgatttaatt aaaaacggac
    13081 acataaatac ccttgcccgt cgggcagtgg ctgccggata cgacgcactg gaagtcctgc
    13141 gcatatgctc actcaatccg gttctacatt acaaactcga tgtgggattg cttcagcaag
    13201 gtgacgatgc tgattttctg gtggttaata atctgacgga ttttgacgtg aaacaaactt
    13261 atattcgcgg tttgaaagtt gctgaaaaca acgcggtact ttttccacgt tatattccta
    13321 ataaactgat caataacttt aaagccacac cactacagcc cgaacaactt cgcgtccaac
    13381 cttcgggcga tcaactaaaa gtaattgtgg tggacgatgg acagctgttt acacacagcg
    13441 aattggtagt tcctaaaatc gacaacgaca atgttatttc ggacactaca aacgacatac
    13501 ttaaattggt agtttacaat cgttatcagg acatgaaacc tgctatagga tttattaaaa
    13561 acatcggact taaagaggga gctcttgctt ccactgtttc acacgatagc cataatatag
    13621 tggctgtagg cacctccgac gaagaaatcg tatcggcaat caatcatatc atagccagta
    13681 ccggtggtat tctggcttgt aacaaagaca acgttttgct actacctttg cctgttggtg
    13741 gtttaatgtc ggccgaagag ggtgatgtga ttgcccaaaa atatgaagaa cttgatgccg
    13801 cggtaaaaca aatgggatcg cagctgaaag ccccttttat gaccgttgcc tttatgtcgt
    13861 tgcttgtaat tccaaaactt aagcttagtg atcaaggact tttcgatgga cgaacttacg
    13921 cgtttaccga cctaatgata tagtattcag caacaaaaaa agtcagttca ggtctatctg
    13981 aactgacttt ttttgttgtt ttttattcgt gtaaattgat attgaattta atttgaaaat
    14041 cactattgcc cgataaaatt ctgatttctt aaattgtatt atatttctca ctttgaaatg
    14101 acagtcatta ttctatcttt tggagtggag ttcaaaaatc tctataaatc acagtcatcc
    14161 tgagtgtagc gaagaaactt aatatttttt actcgacaaa attatctgga aatacttttg
    14221 cctgtaccac gtttcaatta tcggcatatc acgcatttcg ctttacctta acggcgataa
    14281 aatcatattt ttcattattt cgcaaaagca attgtttcat tctgttctga cgaagaatat
    14341 acagacaaca gttctaatgg agcaggtgta tgcataaccg gctttgtttt tattaaatgc
    14401 gtcaggttac cgaagcggga gtggcaacga ttgaaatccc ggtaatcaat acggatttat
    14461 acctgtacaa cttccgaagt ctgccatcct tcacaattgt tgcctgcctg ggtggagtgt
    14521 gtgatatgac ttagtcatag accgaaaagc aatacgtacg ttcggaatac ctgtacattg
    14581 acaaaatgcg aggaatatct caaagcgact ggatgcctgc cacgggtacg cagcccggct
    14641 tttccatccg gctgtactgt cccctgaaac tgatgccgat aaccgatgat gtgaccatgc
    14701 tggtgtgtct tggggtggag ttcgggaaaa acggctacgg caacaccact gaacctgtaa
    14761 aatacgccgg aacagaaaaa atagtgcggg ttggataaga tttgacaaag tgaaacaaaa
    14821 aaacgggtaa attattaggt tcgagttttc acctgattac aataataaag gagcagcgat
    14881 ttcaatccct gctcctttat tattgtattg tccggcaaac gtttttcagc cctcttttac
    14941 ccgtatttct gaagttactt ctatcgcttt tctgaataga gcttccacac cgcagtaggt
    15001 ttcttccgac attttcactg ccttttccag cttttccatc ggcaggtctt taccggtaaa
    15061 ttcatacact acgtgcattc tttcgtagta tttcggatgc tcatcgcgca tatcggcttc
    15121 cacgctgatg ttacatttcg cgatattcac acgcattttt tttagaatgg agaccacatc
    15181 catccccgta cagccggcca gcgccacaag cagcaatttt ttgggactcg taccgagatt
    15241 atcgcctccg gctgccagcg gtgcgtcatt tatgattttg tgttcgtcta ttgtggattc
    15301 aaaagtgaga ttgcctttcc attcggaagt aataacatgt ttcatttaaa aaaaattaga
    15361 gtgttataaa aaagtgaaat tataccatca aaaaaaaccg gaattttgca tttgacatgt
    15421 aaaaatcggc tcttcttccg gttttttgat aaaacatttc aggcagggta aaagttttcg
    15481 gaaaaaattt tcgataaaaa ataaaaaaaa gaaaggcaat tttcagctga attaacatac
    15541 aaattgccct aaaaattata aaattattca gattcaattt tcctgctgtc tatttgtaat
    15601 cttttcagaa attgaggatt acgagcgcaa cggccggcgg tttgcaggct tgaaattaat
    15661 tcataaattt gtaccggata tttactcaac acgtatttta tatgcatccg atagcttcat
    15721 ttcaggaact gacatcgcat ctgaccgaat cacagcatcg caaacgcatg gcagtggcca
    15781 atgcagtgga ttcacacacg cttgatgccg tgatcaacgc tgtaaatgcg ggcatcgtag
    15841 aggcttatct ggtaggcgac gtggctgaaa ttgagtccta tctgctcgaa cagccggata
    15901 tttcatcata cattcacatc attgataaac ccgatgcggg cgaagcaacg cttgaagcgg
    15961 tacgcatggt gaaggatggc gaatgcgata tcctgatgaa agggctggtg aataccgatg
    16021 tgattctgcg ggctatactc gacaaacaaa aagggctctt gccacccggc aatgtgctga
    16081 cctacaacgc cgcactcgaa attccgggtt accacaagct gattttcttt tcggacccgg
    16141 ccgtgatacc ctaccccacg ctcgatcagc ggaaagccat gataaaatac gccatcaaca
    16201 cctgctataa attcgggctt tcaaaaccca aagtggcatt aattcatgcc acagaagtag
    16261 ccagccctaa aatcacgttt atgcaggatt acctggatat catgcagatg tggcgtgcag
    16321 gtgaatttgg cgacgtgatc atcgacggac cgctggatat tttcctggct ctggacgccg
    16381 agcggggcgg tatcaagaaa gtaccttcac cgttgctggg cgacgccgat gtgcttattt
    16441 tccccgattt tgccgctgcc aatgtctttt acaaaggagt ggcacttttt gctaacgctc
    16501 agatgggcgg tattctgcaa ggcacctcca aaccgtgtgt actcacctcg cgcagcgaaa
    16561 ccgccctttc aaaattctac agcatcgccg tggcctgcat cctggcataa atgtactgaa
    16621 tgttatattt gattttcggg tgcgacggtt ttcaaccgcc gaaaaaataa acggttaccc
    16681 cgatagctat ctgggcgctt cacccgtgag gtttgtacaa acatttcggg tgaaaacagc
    16741 taaaaaccga tttacccaaa gatatttttc gtatctttat ccgctgatta cggagcatgc
    16801 gtggcatgat atggagcacc cgcaaattat acggacttca agtttgagtc acttatgcag
    16861 tttacttcat tctataaaca actgataacc aactgcaata ctgtaaaaaa ttacattcaa
    16921 gaaaaaaaac tacaaatatg gacaacaaac aaaatcagga aaatcagctg aacattgaat
    16981 tatcggctga agtagctgaa ggaacttact caaa
//
LOCUS       DAORKP010000032        16918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_221_length_16918_cov_9.281978, whole genome shotgun sequence.
ACCESSION   DAORKP010000032 DAORKP010000000
VERSION     DAORKP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221_length_16918_cov_9.281978"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLN75_03190"
     CDS             complement(<1..689)
                     /locus_tag="PLN75_03190"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRG03155.1"
                     /translation="MKKIFLYLFTLLVGSFAFYSCEDPYAGQTVADPTIYEQEALQDA
                     NFKATVLTNPLVITNDKLATNFSLLKIDSRPTLIDSAAHIEYKVLLSNTQDFATFKLL
                     PTTYGGTSGSDLVASYTVINDTLKALNPALGSHDAFARVLAYIVSGGTRALYTTSTMP
                     FKVTSFNYAPKVVNDTVIAIKDQELFYNVLANDSDVEGDQLLITAVGTPAHGKASFSG
                     GSILYTPNAGYV"
     gene            complement(766..1986)
                     /locus_tag="PLN75_03195"
     CDS             complement(766..1986)
                     /locus_tag="PLN75_03195"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HRG03156.1"
                     /translation="MKNINILILGFVLMFGFNACQEDPIVTINPSAETGDMTFQLNQA
                     RYTGYTYELLESNNDLDMDALYASQPEYGFTAAVTYYIETSFTENMADAVELPSSVQG
                     EKVSINTKELNRAILQLYKGQMPNPTVAKDMYFRLKAVVSQALPTPLDTVPTVKPLYS
                     NVVKLKVLPYFIEDKVSFNKAKKLTYWYIVGTLGKGDHWINDASGVGHSTIPLGVLEG
                     DLYDGEGNGTFSYTGYIKTSQNFKIVRDLGSWNVQWGNADGKAINKPILKLKDGDAEG
                     TDFQVPEDGYYNITLNSINNTLKIEKVQITPAVHPNMGLVGTINDWGGGGADVVLQPF
                     QAENNHYWYATYTVAAAGEVKFRVDNKWDLNWGNSTFPQGLATPGGPNVPIAAGTYTI
                     LFNDLDGFYQFIKK"
     gene            complement(2017..3591)
                     /locus_tag="PLN75_03200"
     CDS             complement(2017..3591)
                     /locus_tag="PLN75_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03157.1"
                     /translation="MKINKKFHLYLASLVVAIGLTSCVQDLDIQPIDPSVVQTFSEPE
                     VFTKVYAAWALTGQQGAAGNNDIDIDDEGRFSLYRTLWVTQQLPTDETVCAWGDIEVV
                     TMNRSAFTSTNPALKGLYARLYFVVTIANHYLEQTNASADDATIKVKRAEVRYLRALA
                     YYYLMDVFGNVPFTTEVKADAPTQIKRADLYTWIETELKEVEPELSAPKQAPYYRIDK
                     AAAWMLFARMYLNAQVYTGTPKWNEAAIYADKVMKSGYTLATNYKYLFMADNAGTNDG
                     STVNDAPKEIIFPIAADGVKTKNWGSSIFAINSTRTTGMPTWGTTGGWGGNRARVKLI
                     RKFFPGDVPEAEDLSAAANDNRAMFSSKERTLNISDVTIFKEGISVQKFWNLRADGKP
                     TSDMEFPDTDIPFMRAAEAYLTYAEAVVRGATPIGGYSALQAVNAVRARAKASVLVAV
                     DKDVILDEWSREFYFEGRRRTDLIRYGYFTGNAYNWDWKGGVANGTALPGYLNLMPLP
                     ADDLNANSNLEQNPEY"
     gene            complement(3617..>4531)
                     /locus_tag="PLN75_03205"
     CDS             complement(3617..>4531)
                     /locus_tag="PLN75_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03158.1"
                     /translation="SLLNNRFSASLDYYYRVTNDLINVTPVPAGTNFRNKVIQNVGSL
                     NNQGVELMLNAKAISTKDVTWDWNYNITYNRNHITKLTTGDQEGYYVPTGGIFQGSVQ
                     AHAVGYPASSFFVYQQVYDNGGKPIEGLYVDRNGDHKITEDDKYFFHNASPDFTMGLA
                     SKLIYKQFDFGVSARANIGNYVYNSVAAERMNVGRNGIWSPLAFFENKVSSAFDTNFT
                     GGSGITFMSDYYVQNASFARIDNITAGYSFNKLLGGVISGGRVSATIQNPFVFTKYKG
                     LDPEIFGGIDGNIYPKPVVTVIGLTLNF"
     assembly_gap    4532..4631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4632..>5899)
                     /locus_tag="PLN75_03210"
     CDS             complement(<4632..>5899)
                     /locus_tag="PLN75_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRG03159.1"
                     /translation="TNAQDMISGKIAGVVVTQNGGTPGGSSTIRIRGGSSLNASNDPL
                     IVIDGLTIDNNGIQGVANQLSTVNPNDIESFTVLKDASATAIYGSRASNGVILITTKK
                     GKSGSKPKLSYNGNFNVNTIGKYMDVMTGDEYRDYANKLYPGNTDVLKNLGTENTNWQ
                     SEIYRTALGHDHNLNLTGGFKNLPYRVSAGYTKQDGIIKTSSFERFTGAVNLNPSLLD
                     DHLKININAKGMYVKNRYADAGGVVGNAVRFDPTHPVRSTAEPYASKLDGFWQWATFA
                     GDQFLSVNRDAPANPVSMLEHRDDRSKAWDLLGNIDIDYKLHFLPDLRYHLNLATNIS
                     HGEQNTWISPYSFSNLVGGGYDGYNNQDKWNRQLSTYLQYTKELETQNFDVMAGYEWQ
                     RYHREGTYDGKSISKPDYIYQTEPVSWATH"
     assembly_gap    5900..5999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6452..7471
                     /locus_tag="PLN75_03215"
     CDS             6452..7471
                     /locus_tag="PLN75_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRG03160.1"
                     /translation="MAKTQITIIDIARELNISPSTVSRALRNHPDISRKTVEIVKECA
                     ERLSYKPNEMALNLRLGRNNIIGVIVPEIVHYFFSSIVQGIEETASAEGFNVMLFRSE
                     ESYEKELKITESLINARVAGVLANLSKETDRYEHYQQILDNDIKLVFYDRICIGIITD
                     RVVVDDYAGAFSAVEHLISTGCRRIAFYSSPLHLEISKNRRNGYLDALRKNSIPVDES
                     LIFVCDTREKAMELTPHVLSLPEPPDSFFAINDHTATGILYAVKKAGLRVPEDISICG
                     FSGGDLALACDPMLTTVEQHGYEVGKTAAKLLIDKIKGITHGQYTNRIIKTKLMVRGT
                     TKTAP"
     gene            7652..9133
                     /locus_tag="PLN75_03220"
     CDS             7652..9133
                     /locus_tag="PLN75_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG03161.1"
                     /translation="MSFGFLGIQFGFALQNANVSRIFESLGAEIDKIPILWLAAPVTG
                     LIIQPIIGHWSDKTWNRLGRRKPFFLTGALLASTILLLMPNSPSLWVAAGLLWIMDAS
                     INVSMEPFRAFVGDMLPSSQRTLGFSMQSFFIGLGAVAGSAMPYILTNWFNVSNVPVG
                     DQVIPDTVKWSFYVGGAVFITSVLITILTTREYPPEVLSSFHSESTAPVIMEGTPADE
                     VAPGKFNRLAFIFGISGLLIVSAVYLFHMDLQIYVLGGLFLLIAALFMAVGFLRKKGK
                     SNGMTEIMTDLINMPKTMGQLALVQFFTWIALFSMWIYATPAVTQFHFGTAETKSELY
                     NTGANWVGILFGAYSLFAAAVAFLLPVMARYISRKYTHLIALVIGGLGLISMMFFKNP
                     DYLLISMFGIGFGWASILSMPYAMLTGSLPAAKMGTYMGIFNFFIVLPQILAATILGS
                     ITKHLFNGQPIYTLVFGGCCMILAGVFTLIVNDADDPIHKKNR"
     gene            9265..11142
                     /locus_tag="PLN75_03225"
     CDS             9265..11142
                     /locus_tag="PLN75_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRG03162.1"
                     /translation="MNKINNVILAIILTSLLPLNVHSQKVAQGGFKGLQHIEPTNWWT
                     GMKNPELQLMLHGTDMASSELSIDYPGVSVTQIKRTDNPNYLFVYLNITPETRPGIFK
                     IILKKGRKKQVVNYELKERTPNSANRESFSEKDNVYLLMPDRFANGNSLNDSVKGYHQ
                     GVNRSNLSHRQGGDIEGIIGKIPYLADLGITALWTTPLFDNNDTNYSYHHYGCTDYYK
                     IDPRLGKNEDYLRLSQTAGNHGIKMIIDVVPNHCGGSHWWMKDVPSSDWFNVWKTYTS
                     SNYRMTAWTDPHASETDRYKLTHGWFAPNMPDLNLSNPLLYDYLKQVYIFWIEYAQIA
                     GIRVDTYPYNDIKTAARFIQTIRDEYPNMNVVGECWVKTPAETAYYQSGNNNKDGFDS
                     RLQSVMDFMLKDKLQEAFKENEGWDTGTAKFYSHFAQDFVYPNTNLIMNFVDNHDIDR
                     LSTAYKNVNEYKMALAMLVTVRGYPQIYYGNEIMLEGISGDYQGHRFTFPGGWKNDKR
                     DAFTPAGRTKEENEVFDYLKKLLHYRKNNPVLQNGKMMQFIPENGVYVFFRYNDEKTV
                     MVITNNNDHATVLDTKRFAERIKDCEMGVEVTSKQAVDMKNQVEIPGKTVLVLELGFW
                     R"
     gene            11325..13142
                     /locus_tag="PLN75_03230"
     CDS             11325..13142
                     /locus_tag="PLN75_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HRG03163.1"
                     /translation="MSTTNNSPRTPKSPLRGVGGLFHNFGEKLQTAITRFPLTFACIV
                     GVAVLFVSAIHTNTDNIYTKWIMGGIVSAFASLSFYLFAENRLGKLVANTVNIALIGL
                     IIWLFSYFSKNITDANAAQFVMLTVSFFLALFFAGYLTEKNSLHWWDNLQKTLFHLII
                     SAFFAGILMGGLSLALVSLDKLFGLKIDDKMYAYLAVFCFVLFMPSYFLSQLSEKTAS
                     DESFSISYPAIFKILGLYILLPILAIYTLILYGYLFKIIASWELPNGWVSWLVSILGL
                     AGFLTIVLIHPIAPPPPKGGISASLNKTDAVSKSVSKSPFGGLGGFFLRFFPLILLPL
                     LVLMFVGIVRRFSDYGITINRLLVLILNIWFFGISIYLFISHSRQPKWILISFAIVAF
                     LSAVGPWSVVNVTEKSLKKEFSALLTEAGWTNSKENQIKTLDKEKQTRLYDVAYYLQR
                     TYGIESIRPMFSELSETAGVENLMIKLGISDPQIAKNEHISVNSGEKSYQFDISNYNH
                     AIHLRNTYNSDVLFKSDSINIYLENYVVKCIWNNKVTLIPLESTIEKSVSMTGKSTNP
                     EEIIIKKDDYKLIIFYLDGQRKADGEVKINHFNGLLLMK"
     gene            13265..14434
                     /locus_tag="PLN75_03235"
     CDS             13265..14434
                     /locus_tag="PLN75_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG03164.1"
                     /translation="MEGLFAYFNKLLRETNLDFRRYLNSKIDWNSRLIGLLGARGVGK
                     TTLVLQHIKENLNPNEALYVTAEDFYFAEHRLLDLANDFSKMGGKYLFIDEIHKYKDW
                     SKELKLIYDYHAELKVVFTGSSILDINKGASDLSRRAIMYQMYGLSFREYLQLFHNIS
                     APVFTLEEIISHKVEIPELKHPLPLFQSYLANGYYPFAMENGYDFRLTQILNQTLESD
                     IPQYAGMNVATGRKLKQLLMIVAKSTPFKPNFSKIAQILEASRNNLADYFLFIEEAGL
                     IMQLRDETGGIRGLGKVEKVYLENTNLIRNLAQENANTGNIRETFFVNQTRVYSDVIS
                     SKTSDFLIDDMTFEIGGKNKTQKQIQNTPKSFLVKDDIEHGYLNIVPLWQFGMGY"
     gene            14572..>16918
                     /locus_tag="PLN75_03240"
     CDS             14572..>16918
                     /locus_tag="PLN75_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRG03165.1"
                     /translation="MKTKFPILIVFILILASCQKPALNLPAESENNLNLNSLLTLPMG
                     ENLIHTQDFILNPGEIDSVSSPEIPVALTENKSKIMLTLRPETSNFFTVNIWIKGVPY
                     AIPCRKTDKVDYIFSFDPQGKKYGRVQIAGQMNDWAPNLSPDLQLNDSGKYVATLNLS
                     PGTYLYQMVLDGDQNHDANNPNKVDNGYGKFNSILQVAGNSDKFPVLTTEKIEGNKFS
                     VSTQNDVKKVYAYWQNYLLPENFVSFENGKITVNIPKEAGKIDRSFIRIWAENEWGVS
                     NDVLVPLAKGKVLNKSSEINRHDRHAQIMYFMLVDRFKNGNTANDCPMNRPDVHPKVD
                     FFGGDLAGLQQVIDDGYFKKLGVNTLWISPLNQNPNEPYGFYAPKNTKFSGYHGYWPV
                     SFSKVDSRFGSNQELKELVTNAHAKNMNILLDYVAHHVHELNPLYKNHPEYFTSLYLP
                     DGTLNTERWNDYRLTTWFDTFMPTIDFANQQVVEMMTDSALFWLNEFKLDGFRHDACK
                     HVDLPFWRTLTKKIKENNKGKNLYQIGETYGSPELINSYLNTGMLDGQFDFNVFEDAN
                     TSFAGVGVPDLQRVNAVLQSSLKTYGAHNLMGYISGNHDKPRFMALASGDLKFGEDSK
                     AAGWNREIGITDSTAYDKMALMQTFILTIPGVPVIFYGDEIGLTGANDPDCRRMMKFE
                     AWNKREKKLFDKTATLAKLRNTNPVLVYGDFINLQVTKDSWIFARKYFDKTAIVLINN
                     SNESKEMEVEIPSNIQGNFKANFGSKFTVKGGKLKVSLEPFS"
BASE COUNT         4816 a         3540 c         3569 g         4793 t
ORIGIN      
        1 acataacctg cattgggtgt atagaggatt gagccaccac tgaaagatgc tttaccgtga
       61 gcaggagtgc caacagcagt aatcagcagt tgatcgcctt ctacatcact gtcatttgcc
      121 agtacattgt agaacaattc ctgatccttg atagctatta ctgtatcatt cactacttta
      181 ggcgcataat tgaatgaggt aactttgaaa ggcatagtag aagtggtgta cagggctctt
      241 gtaccacccg aaacgatata tgccaataca cgtgcaaagg catcgtgact tccaagagcc
      301 ggattcagcg ctttcagcgt atcgtttatt actgtatagc tggccaccag atccgaaccg
      361 gaagtacctc cgtaggttgt cggcaacaat ttgaacgtgg caaaatcctg cgtatttgac
      421 aacaatacct tgtactcgat atgagcagcg ctgtcaatca acgtgggacg tgaatcaatt
      481 ttcaacaagc tgaaatttgt agccaactta tcattcgtga ttaccaacgg attggtcaga
      541 actgttgctt taaaattagc atcctgcaat gcttcctgtt cgtagattgt aggatcggct
      601 acagtctgac ctgcataagg atcttcacag gaataaaatg caaagcttcc taccaacaat
      661 gtgaacagat ataaaaatat tttcttcatg tttattaagt tttgaattgt aaattttaat
      721 tcagaagatg ctgcctctac cagacagcaa cttctgattt tttacttatt ttttaatgaa
      781 ttgatagaat ccatccaaat cgttaaatag aatagtatag gtacctgcag caatcggaac
      841 atttggtcca cccggtgtag ccaatccttg cgggaaggta ctgttacccc agttcaaatc
      901 ccatttattg tcaaccctga attttacttc tccggctgca gccacagtat acgttgcata
      961 ccagtaatgg ttgttttcag cctggaatgg ttgcaatact acgtcagcgc caccgcctcc
     1021 ccaatcgttg attgtaccta ccaatcccat attaggatga actgcaggag taatttgcac
     1081 tttttcaatt ttcagtgtat tattgattga gttcaatgta atgttataat aaccatcttc
     1141 cggaacctgg aagtcagttc cttctgcatc gccatctttc agtttaagaa tcggtttatt
     1201 gatggctttt ccgtcagcat ttccccactg tacgttccag ctacctaaat ctctgacaat
     1261 cttgaagttt tgtgatgttt tgatataacc tgtgtaactg aaggttccat ttccttcgcc
     1321 gtcatataag tctccttcta aaactcccaa tggaatagta gaatgtccta caccggaagc
     1381 atcgttaatc cagtggtcac cttttcctaa tgtacctaca atataccagt aagtgagttt
     1441 tttagcttta ttgaagctaa ccttgtcttc tatgaagtag ggaagaactt tgagtttcac
     1501 tacattggaa tacaaaggtt tcactgtagg tacagtatcc aaaggagttg gtaatgcctg
     1561 acttactact gctttcagac gaaaatacat atccttggca acagtcgggt taggcatttg
     1621 acctttgtac aactgaagga ttgccctgtt caattccttg gtgttaatgc ttaccttttc
     1681 accctgaaca gaagatggca actctacagc atcagccata ttttctgtaa aagaagtttc
     1741 gatataatac gttacagctg ctgtaaatcc atattcgggc tgtgaagcgt acaaggcatc
     1801 catatccaga tcattatttg attccagcaa ttcatacgta tagccggtat atcttgcctg
     1861 gttgagctgg aaagtcatat cgccggtttc ggccgatgga ttgatggtaa caatcggatc
     1921 ttcctgacag gcattgaatc caaacatcaa cacgaaacct aatatcaata tatttatatt
     1981 tttcatttct acttgtgtta ttaagttttt cattttttag tattccggat tttgctccag
     2041 gtttgaattt gcattcaggt catcggccgg aagtggcatc aggttgaggt atccgggaag
     2101 tgcagtgcca ttggctacgc cacctttcca atcccagttg taggcatttc cggtgaaata
     2161 tccgtagcgg atgaggtccg tgcgacggcg accttcgaag tagaattctc tggaccactc
     2221 gtctaaaata acgtctttat caacagcaac gagtacactt gcttttgctc tggctctcac
     2281 agcattcaca gcctgaagcg ccgagtaacc tccaatagga gtcgctcctc ttaccacagc
     2341 ttcggcatac gtcagataag cttcagcagc acgcataaac ggaatatccg tatcggggaa
     2401 ctccatgtcg ctggttggtt taccgtcagc acgcaggttc cagaatttct gtacagaaat
     2461 accttctttg aaaatagtta catcggatat gttcaacgtt ctttctttgg atgagaacat
     2521 ggctctgttg tcattcgcag cagcgctcaa atcttcagct tcaggaacat caccagggaa
     2581 gaatttccgg atgagtttaa cccgggctct gttaccgccc cagcctccgg tagttcccca
     2641 ggtaggcata ccggtagtac gtgttgagtt gattgcaaat attgatgatc cccagttttt
     2701 ggttttcaca ccgtcggccg caatcgggaa aatgatttct ttcggagcat cgtttacggt
     2761 tgagccatca tttgttccgg cgttgtctgc catgaacagg tacttgtagt tggtagcaag
     2821 cgtgtatcct gatttcatca ctttgtcggc atatatagcc gcttcgttcc atttgggtgt
     2881 tccggtatat acctgagcat tcagatacat gcgggcaaac agcatccagg cagcagcttt
     2941 gtcaatgcgg tagtatggag cctgtttagg tgctgaaagc tccggttcaa cttctttgag
     3001 ttcggtttcg atccaggtgt ataaatcagc acgtttaatc tgagtaggag catcggcttt
     3061 tacctcggtg gtaaacggaa catttccaaa tacatccatc aggtagtagt aagctagtgc
     3121 ccgcaggtaa cgaacttcgg cacgtttcac cttaatagtt gcgtcgtcgg cagatgcatt
     3181 cgtttgttcc aggtagtgat tagctattgt aaccacaaag taaagacgag catacaatcc
     3241 tttcaatgca gggttggtag atgtgaaagc agatctgttc attgttacta cttcaatatc
     3301 gccccaggca caaacggttt cgtcggtagg taactgctga gttacccaga gtgtgcgata
     3361 cagagagaat cttccctcgt catcgatatc aatgtcatta tttccggcag ctccctgttg
     3421 tccggtaagt gcccatgcag catatacttt tgtaaaaact tccggttcgc tgaaagtctg
     3481 tactacagat gggtcaattg gttgaatatc cagatcctgt acgcaagatg taagcccgat
     3541 agcaacaacg agcgaagcca gatacagatg gaatttttta tttattttca taactttttt
     3601 ttgaatgttg agttgattag aaattaagtg ttaatccaat gactgttaca actggttttg
     3661 gatagatatt tccgtcaatt cctccaaaaa tttcaggatc aagtcctttg tattttgtaa
     3721 atacgaacgg attttgaatg gttgccgata cccgaccgcc tgaaattacg ccaccgagta
     3781 acttattaaa tgaataaccg gcagtaatat tatcaatacg ggcaaatgaa gcattctgta
     3841 cgtaatagtc tgacataaag gttataccac ttccgcctgt aaagttagta tcgaaagctg
     3901 acgacacttt gttttcaaag aaagcaagcg gcgaccagat accgtttcga cctacattca
     3961 ttctttcggc ggcaactgaa ttgtacacgt agttacctat gttagcacgg gctgacactc
     4021 cgaagtcaaa ttgcttgtag atcaacttgg aagccaatcc catggtgaaa tccggagatg
     4081 cattgtggaa gaagtattta tcgtcttcgg taattttatg gtcaccgttt ctatccacgt
     4141 acaacccttc aattggtttt ccaccgttgt catacacttg ttggtaaaca aagaatgaac
     4201 tggcaggata acctacggcg tgtgcctgaa ctgaaccctg gaaaataccg cctgttggca
     4261 cataataacc ttcctggtct ccggttgtaa gttttgtaat gtggttgcgg ttgtaagtga
     4321 tgttgtaatt ccaatcccag gttacatcct tggtagagat tgcttttgcg ttcagcatca
     4381 gctcaactcc ctgattgttc agtgaaccta cattctgaat tactttattt ctgaagttgg
     4441 tacctgcagg aaccggagtt acgttaatca aatcgttggt aacacggtaa taataatcaa
     4501 gtgatgcaga aaatctgttg ttcagcaaac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nttgtgagta gcccacgaaa caggttcagt ttgataaata taatccggtt
     4681 ttgaaatgga ttttccatca taggttcctt cccgatgata acgttgccac tcgtaaccgg
     4741 ccataacatc gaaattctga gtttccagtt ctttggtgta ttgtaaatat gtactcaact
     4801 gacggttcca tttgtcctga ttgttgtaac catcataacc gccacctacc aggttggaga
     4861 atgaatatgg agaaatccag gtattttgtt caccgtgtga gatattggta gccagattca
     4921 ggtgatagcg caaatcaggc aagaaatgga gtttgtaatc gatatcgata ttaccaagta
     4981 aatcccatgc ttttgagcgg tcgtctctgt gttcgagcat acttacggga ttggctggag
     5041 catctctgtt taccgaaagg aattggtctc cggcaaaagt agcccattgc cagaatccat
     5101 ccagtttgga tgcatacggt tcagctgttg aacgaacagg atgagtcgga tcaaaacgta
     5161 cggcgttacc tactactcca ccggcatctg catagcggtt tttcacatac atccctttgg
     5221 cattgatgtt gattttcaaa tgatcatcaa gtaaagaagg atttaagttg acagcgccgg
     5281 tgaaacgttc gaaacttgaa gttttgataa taccgtcctg cttggtataa ccagctgaaa
     5341 cgcgataggg cagatttttg aatccaccgg tcaggttgag gttatgatca tgaccaagag
     5401 ctgtacggta aatttcactc tgccagttgg tattttcggt accaaggttt ttcagaacgt
     5461 ctgtatttcc gggatagagt ttgtttgcat agtctctgta ttcgtcaccg gtcatcacat
     5521 ccatgtattt accgatggta tttacgttga aattaccgtt atagcttaat ttcggttttg
     5581 aaccggattt acctttttta gtagtgataa gaataacacc gttggatgcg cgtgaaccgt
     5641 aaatagcggt tgcagaagca tctttcaata cagtgaaact ttcaatgtcg ttgggattga
     5701 cagtactcaa ctggttggca acaccctgta taccattatt atcaatggta agaccatcga
     5761 taacgataag cgggtcgttg ctggcgttca gtgatgatcc gccacggata cgaatggttg
     5821 aactaccacc cggcgtaccg ccgttttgag ttaccacaac tccggcaatt ttacccgaaa
     5881 tcatgtcctg agcattggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     6001 tttctccggc tgcatctctt actacccccg taacggattt cttttgtgca ttggcactga
     6061 agccaatcac aagtgtcagc actacgatca gtaatgatcg catttttaaa ttaaatcgtg
     6121 cgtccttcat gcaattctgt ttttttgagg ttgataatta ttgtttattt aatgatttta
     6181 tttcaatcac atctggctaa tttcagaaaa ccattttgtg ttttttctgg aatatcgtag
     6241 caaaattata cttttgggaa attttaacaa caagaataac agttaataag aagtttttcc
     6301 agtgcaaacg tttgcaaaag cattggaaat tcaattcaat gaattaattt tacattctga
     6361 tattctatta tacatttgca atattgtaat tttcagagtt ctgagttcaa aaaatttcgg
     6421 tattaaaaac tgacttcatt cattttttat catggctaaa acacaaatta ctattattga
     6481 catcgcccgg gaactcaaca tatctccctc tacggtttcc agagcactga gaaatcatcc
     6541 ggacatcagc cgaaaaaccg tagaaattgt aaaagaatgt gccgaaagac tcagctataa
     6601 accgaacgaa atggcactga atctgcgctt aggacgtaac aatattatag gtgtaattgt
     6661 tccggaaatt gtacattatt ttttctcatc catcgttcag ggaatagaag aaacagcatc
     6721 tgccgaaggt tttaacgtga tgttgtttcg ctcggaagaa agttatgaaa aagaacttaa
     6781 aattaccgaa tcgctgatca atgcccgtgt ggccggagtg ctggccaacc tttcgaaaga
     6841 aacagaccgc tacgaacatt atcagcaaat actcgacaac gatatcaaac tcgtatttta
     6901 cgaccggatt tgtattggga tcattacaga ccgggtagtg gtggatgact atgcaggagc
     6961 attttcggca gttgaacatc tgatcagtac aggttgccgc cgaatagcct tttacagctc
     7021 gccattgcat ctggaaatat caaaaaacag gagaaacgga tacctggacg cacttcggaa
     7081 aaacagtatt ccggtcgatg aatcgctcat attcgtatgc gatacccgcg aaaaagccat
     7141 ggaacttact cctcacgtac tatcacttcc tgaacctccg gattctttct ttgctatcaa
     7201 cgaccacact gccacgggca ttctttatgc cgtaaaaaaa gcgggactgc gggttccgga
     7261 ggatatttcc atctgtggat tttcgggcgg cgatcttgcc cttgcctgcg atccgatgct
     7321 taccacggtg gaacaacacg ggtacgaagt ggggaaaaca gcagctaaac ttctgataga
     7381 caaaataaag ggtataactc acgggcaata caccaaccgg attattaaaa caaaactgat
     7441 ggtgagaggg acgacaaaaa cggcccccta aatcccccaa gggggacttt gaggtttgca
     7501 aaacaaatta tagtaataga aaaaaatata atttttcctt aaaatttata tgaataaaca
     7561 cataaaccac aatcaaccgg actcaccaaa agtcacctcc gggaaattta aggggcaaaa
     7621 accccgttta agcttctggc aaatatggaa catgagtttc ggttttctgg gcattcagtt
     7681 cggatttgct ctgcaaaatg ccaatgtaag ccggattttc gaaagtcttg gagccgaaat
     7741 tgacaaaata cccattctgt ggctggctgc acccgttaca gggctcatca ttcaacccat
     7801 catcggacac tggagcgata agacatggaa caggctcgga cgtcgcaaac catttttcct
     7861 cacaggagcc ttgctggctt ccaccatcct gctactcatg cccaattcac cttcgttatg
     7921 ggtggcagcc ggactgctgt ggattatgga cgcttccatc aacgtttcga tggagccgtt
     7981 cagggctttt gtaggcgata tgttgccgtc atcccagcga acgcttggat tttcgatgca
     8041 gagtttcttt attgggctgg gtgcagtggc cggttcggct atgccttaca tacttaccaa
     8101 ctggttcaac gtttccaatg tgcctgttgg cgatcaggtt atccccgata cggttaaatg
     8161 gtcgttttac gttggtggag ctgttttcat tacctcagta cttattacca tactgaccac
     8221 cagggaatat ccgcccgaag tgctcagcag tttccactcc gaaagcaccg cacccgtcat
     8281 tatggaaggc actccggccg atgaggttgc tcccggaaaa tttaaccgcc tggcgtttat
     8341 ttttggtatt tccggtttgc tgattgtcag tgctgtgtat ctgtttcaca tggatcttca
     8401 aatttatgtg ctgggtggat tgtttctgct tatagcggca ttgttcatgg cagtaggttt
     8461 tctccgaaaa aaaggaaaaa gcaacggtat gaccgaaatc atgactgacc tcatcaatat
     8521 gcccaaaaca atgggtcagc ttgccctcgt gcaattcttc acctggatag cgctgttttc
     8581 catgtggatt tatgccactc cggctgtcac gcagtttcac tttggcaccg ccgaaaccaa
     8641 gtcggagttg tacaacaccg gtgccaactg ggtgggaatc ctgtttggtg cttacagtct
     8701 gtttgctgcg gcggtagcat ttctcctgcc ggtgatggcc agatacattt cccgcaaata
     8761 cacacatctg atagctctgg ttataggcgg gctagggctt atctccatga tgttttttaa
     8821 aaatcccgac tatctgctca tcagtatgtt cggaattgga ttcggatggg caagtattct
     8881 ctccatgccc tacgcgatgc tgaccggttc attgccggct gccaaaatgg gcacctacat
     8941 gggtattttc aattttttca ttgttctgcc gcaaattctg gcagctacca ttctgggaag
     9001 catcacaaaa caccttttca atgggcaacc tatctacact ctcgtatttg ggggttgctg
     9061 catgatactt gccggcgtgt ttaccctgat agtgaacgat gcagatgacc ctatacacaa
     9121 aaaaaatcgc taaattctcc caggggggag aaattttgaa gaatacaaat tcaaatcttt
     9181 tctgatttat ttaacgtttt caaaatttca atcctttgta aattggaact attttcaaga
     9241 aaaaaaaaaa aaacaaaaga gtatatgaat aaaatcaaca atgtaatttt agcaattatt
     9301 ttaacctcgt tacttccttt aaatgtacac agccaaaaag tcgcacaagg gggatttaag
     9361 gggctgcaac atatagagcc cacgaactgg tggacgggga tgaaaaatcc ggaactgcaa
     9421 ctgatgcttc acggcacaga tatggcatcc agcgaattat ccatcgatta tccgggagta
     9481 tcggtaacac agattaaacg gacagacaat ccgaactatc tgtttgtata tctgaatatt
     9541 acgcccgaaa ccagacccgg aattttcaaa atcatcctca aaaaggggag aaaaaaacag
     9601 gtcgtaaatt atgagctgaa agagagaaca cccaactctg ccaaccgcga aagtttttcg
     9661 gaaaaagaca acgtgtacct gctgatgccc gaccgctttg caaatggcaa ttcgctgaac
     9721 gacagcgtaa aaggatatca tcagggtgta aaccgcagca atctgtcgca ccggcaggga
     9781 ggagatattg aaggaataat cgggaaaatt ccgtacctgg ccgatctggg aattacagct
     9841 ctctggacta ctcctctgtt tgacaacaat gacacgaact attcatacca tcattacggc
     9901 tgtacggatt actacaaaat tgatccgcgc ctcggaaaaa acgaagatta cctgcgcctt
     9961 tcacaaactg ccggaaatca tggtatcaaa atgataatcg acgtagtgcc caaccattgc
    10021 ggaggctctc actggtggat gaaagatgta ccttccagcg actggttcaa tgtgtggaaa
    10081 acctacacat cctccaacta ccggatgacc gcctggactg atcctcatgc cagcgaaact
    10141 gaccgttaca aacttacgca tggctggttt gcacccaata tgcccgatct gaatctgagc
    10201 aatccattgc tttacgatta cctgaaacaa gtgtacatct tctggataga atacgcgcaa
    10261 atagccggaa tacgcgttga tacctaccct tataacgata taaaaacggc tgccagattt
    10321 attcaaacca tacgcgatga atacccgaac atgaatgtgg tgggcgaatg ctgggtgaaa
    10381 actccagccg aaacggctta ctaccaatcg ggcaacaaca acaaagacgg attcgacagc
    10441 cgactgcaga gtgtgatgga ctttatgctg aaagacaaac tgcaggaagc tttcaaggaa
    10501 aatgaaggtt gggatacggg aacagcgaaa ttttacagcc attttgcaca ggattttgtg
    10561 tatccgaata cgaacctgat tatgaatttc gtggataacc acgatataga ccgcctttca
    10621 acggcttata aaaacgtcaa cgagtacaaa atggcgctgg ccatgctggt aactgtgcgt
    10681 ggctatccgc agatttatta cggtaacgaa attatgctcg aaggcatttc gggtgattat
    10741 cagggacata gatttacgtt tccgggcggt tggaagaacg ataaacgtga tgcatttact
    10801 cccgcaggtc gcacgaaaga ggaaaacgag gtgtttgatt atctgaaaaa actgcttcat
    10861 taccgaaaaa acaatccggt gcttcaaaac ggcaaaatga tgcagtttat tcctgaaaat
    10921 ggcgtatatg tgtttttccg ctacaacgat gagaaaacgg tgatggtcat tacgaataat
    10981 aacgatcatg ccacggtgtt agatacaaag cgatttgccg aaagaataaa ggattgtgaa
    11041 atgggcgtgg aggttacttc caagcaggca gttgatatga aaaatcaggt ggaaatacca
    11101 gggaaaacgg tgctggtact ggaactcggt ttctggagat aacaggacac ggatgacacg
    11161 gattatatgg ataaaaacgg atttttttca aatttttctt gattagaaaa aaagattcgt
    11221 tttacaaaat atttttgctt gaaactgtaa tttttcagac agataatccg tttattatct
    11281 gttgaaatat ttacttttaa acaatcaaaa attaacatcc taaaatgagt acaaccaaca
    11341 actcaccgcg aacaccaaaa tccccgcttc ggggtgttgg ggggcttttc cacaacttcg
    11401 gcgaaaaact ccaaaccgct atcacccgtt ttccactgac ttttgcctgc atcgttggtg
    11461 tggctgtgtt gtttgtatca gctatccata cgaacacgga caacatttac accaagtgga
    11521 ttatgggcgg aatcgtgtcg gcatttgctt cgctttcgtt ttatcttttt gctgaaaacc
    11581 ggctcggaaa acttgtggca aataccgtaa atatcgcgct gattggtctg ataatctggt
    11641 tgttttcata cttttcaaag aacataaccg atgccaatgc agcgcaattc gtgatgctga
    11701 cggtgagttt tttccttgcg ctgttttttg cgggatatct gactgaaaaa aattcgcttc
    11761 actggtggga taatttacag aaaacactgt ttcacctaat aatttcggcg ttttttgcgg
    11821 gaattttgat gggaggattg agtttggcac ttgtttcgct cgacaaactt ttcggactga
    11881 aaatcgacga taaaatgtac gcttacctgg cggttttctg ctttgttctg tttatgccga
    11941 gctattttct atcccagctt tccgaaaaaa ccgcatccga cgagagtttt tccatttctt
    12001 atcctgcgat tttcaaaatt ttaggattgt atattttatt gcctatattg gcgatttata
    12061 ccctgattct atacggttat cttttcaaaa taattgcatc gtgggaactt ccaaacggtt
    12121 gggtttcgtg gctggtatcg atattgggat tggcgggatt tctgacaatt gtacttattc
    12181 acccaatagc ccccccaccc cccaaagggg ggatttcagc aagtctgaat aaaactgatg
    12241 ctgtttcgaa aagcgtttca aagtccccct ttgggggatt agggggcttt ttcttgcgtt
    12301 tttttccact tatcctcttg cccttgcttg tgttgatgtt cgtaggcatt gtgcgccggt
    12361 tttcggatta tggcatcacc atcaaccgac tgctggtact gattctgaat atctggttct
    12421 ttggtatcag tatctacctg tttatcagcc attcgcgtca accaaaatgg atcttaatct
    12481 cgtttgcaat cgttgcattc ctttcggcgg tgggtccgtg gagcgtggtg aatgtgacgg
    12541 aaaagtcgct gaaaaaagag ttttctgccc ttcttaccga ggccggttgg acaaactcaa
    12601 aagaaaacca aatcaaaacc ctggacaaag aaaagcagac ccgtctttac gacgtggcgt
    12661 attacctgca acggacgtat ggaattgaaa gtattcggcc gatgttcagt gagttgagtg
    12721 aaacggcggg tgttgagaat ttgatgatca aacttggaat ctctgaccct cagatagcta
    12781 agaatgagca tatttctgta aattccgggg agaaaagtta tcaattcgat atttccaatt
    12841 acaatcacgc aatacattta aggaatactt ataatagtga tgtccttttc aaatcggaca
    12901 gtataaatat ttacctggaa aattatgtag tcaaatgcat ttggaacaat aaagtaacat
    12961 tgatacctct cgaatcaaca attgagaaat cagtttccat gacgggcaaa tctactaatc
    13021 ctgaagagat tatcattaaa aaagacgatt acaaactgat tattttttat ttggatggtc
    13081 agcgaaaagc tgatggagaa gtcaaaatca accatttcaa cggattactg ttgatgaaat
    13141 aggtgatatt gcactattac agtgcaatat ttctaattat ttgcactttg agagtgcaaa
    13201 taatttccat ttttgcactt aaacagtgca aattgttgta tctttgcaaa aaagacaaga
    13261 aaaaatggaa ggactttttg catatttcaa caaactactg cgagaaacaa atcttgattt
    13321 tcgtcgttat cttaattcca aaatcgattg gaacagtcgg ctgattggct tacttggcgc
    13381 acgtggtgtg ggcaaaacta cccttgtatt acagcatatt aaagaaaatc tcaatcctaa
    13441 tgaagcactt tatgttacgg cagaagattt ttattttgcc gagcacaggt tgctggattt
    13501 ggcgaacgat ttttctaaaa tgggcggaaa atatctgttt attgacgaaa ttcacaaata
    13561 caaagactgg tcgaaggagc tgaaattaat ctacgattat cacgcagaat tgaaagttgt
    13621 ttttaccggt tcgtccattt tggatatcaa caaaggtgct tcggatttga gtcgtagagc
    13681 cattatgtat caaatgtatg gcttgtcttt cagggaatat ttgcagttgt ttcacaatat
    13741 ctctgcacct gtttttacac tggaagaaat catttcacac aaagtcgaaa tcccggaatt
    13801 aaaacacccg cttccacttt ttcaatcgta tttagcgaat ggttattacc cttttgcaat
    13861 ggaaaatggg tacgattttc gcttgacaca aatccttaat caaacactgg aaagcgatat
    13921 accgcagtac gccggaatga acgtagccac aggcagaaag ctgaaacagt tgctgatgat
    13981 tgtggcgaaa agcacgccgt tcaaaccaaa ttttagtaaa attgcacaaa ttcttgaagc
    14041 gagccggaat aacttagctg attatttttt attcattgaa gaagccggac taatcatgca
    14101 acttcgggat gagacgggcg ggatcagggg attgggaaaa gtggaaaaag tgtatttgga
    14161 aaacacgaac ttaatccgga atctggctca ggaaaatgct aatacaggaa atatccggga
    14221 gacatttttc gtaaaccaaa cgcgcgttta ctctgatgtt atttcttcta aaacttctga
    14281 ttttctgata gatgatatga cttttgaaat tggcgggaag aacaaaacac aaaaacaaat
    14341 tcaaaataca ccgaaaagtt ttttggttaa agatgacatt gaacacggct atttgaatat
    14401 cgtcccactt tggcaatttg gaatggggta ctgagaaatt attgacaaaa aagagtacac
    14461 aaaaaacgtt tgcggtactc ttttttcatt ttaaattcat tctttaagtt tttagagatt
    14521 aattggttat ttttgttgtc taaatttatt ttcaactaat aatccagcaa tatgaaaacc
    14581 aaatttccaa tcctgattgt attcattctc atactcgcat cctgccaaaa accggcatta
    14641 aaccttcctg cagaatctga gaacaacctg aacttaaact cgctcctgac gcttccgatg
    14701 ggcgaaaacc ttattcatac gcaggatttt atactaaatc cgggtgaaat cgacagcgtg
    14761 agttcacccg aaataccggt ggcgctgaca gagaataagt caaaaataat gcttacgctc
    14821 cggccggaga cttccaactt tttcacagtt aatatctgga taaaaggcgt tccctacgcc
    14881 ataccctgcc gaaaaactga taaagtggat tacattttct cattcgaccc acagggaaaa
    14941 aaatacggcc gtgtgcagat agccggccag atgaacgact gggcaccaaa tctttcgccc
    15001 gacctgcagc tcaacgacag cggaaaatat gtggcaacac tgaatctgag ccctggtacc
    15061 tatctttatc agatggtgct ggatggcgac cagaatcacg atgccaacaa tccgaacaaa
    15121 gtggacaacg gctacggaaa gttcaactca atactgcagg tggcaggtaa ttcagataaa
    15181 tttccggttt tgactaccga aaaaattgaa ggaaataaat ttagcgtttc cacacaaaat
    15241 gatgtaaaaa aagtctatgc ttattggcag aattacctct taccggagaa tttcgtttcg
    15301 tttgaaaacg gaaaaatcac cgttaatatt ccgaaagaag ccggaaaaat cgatcgctcg
    15361 tttattcgca tttgggcaga aaatgaatgg ggcgtgagta acgatgtgct tgttcctctc
    15421 gcgaaaggaa aagtgctgaa caaatcatcg gaaataaacc gacacgaccg tcacgcacag
    15481 attatgtact ttatgctcgt ggaccgattt aagaatggaa atactgccaa cgactgcccg
    15541 atgaaccgcc ccgacgtgca tccaaaagtg gatttttttg gtggcgacct tgcaggactg
    15601 caacaagtga ttgacgacgg atatttcaaa aaactgggcg ttaatacgct ttggatttcg
    15661 ccactgaacc aaaatcccaa cgaaccttac ggattttacg cgccaaaaaa cacaaaattt
    15721 tcgggatatc acggctactg gccggtttca ttcagcaaag tcgacagccg gtttggttcc
    15781 aatcaggaat tgaaagaact ggtgaccaat gcacatgcaa aaaacatgaa tattttgctt
    15841 gattatgtgg ctcaccacgt tcacgagctg aatccactgt ataaaaatca tccggagtat
    15901 ttcacttcgc tctatttgcc cgacggcaca ctgaacaccg agcggtggaa cgactaccgc
    15961 ctgaccacgt ggtttgatac attcatgccg acgattgatt ttgccaatca gcaagtagtg
    16021 gaaatgatga ccgattcggc acttttctgg ctcaacgaat ttaagctcga cggcttccgc
    16081 cacgatgcct gcaaacacgt agatttgcct ttctggcgta cgcttaccaa aaaaatcaag
    16141 gaaaataaca agggtaaaaa tctgtatcag attggcgaaa cttacggaag tccggagctg
    16201 atcaacagtt atctgaatac cggcatgctc gacggacagt ttgatttcaa cgtatttgaa
    16261 gatgccaaca caagctttgc aggcgtgggt gtacccgatt tgcagcgcgt aaatgccgtg
    16321 ttgcagagca gcctgaagac ttacggtgcg cacaacctga tgggttacat ttccggaaat
    16381 cacgacaaac cccgcttcat ggccctggct tcgggcgatt tgaaattcgg cgaggatagt
    16441 aaggcagccg gctggaaccg tgaaatcggt atcaccgaca gcactgctta cgacaaaatg
    16501 gctttgatgc agacttttat tttaaccatt ccgggagttc ctgtcatttt ctacggcgat
    16561 gaaatcggac ttaccggcgc caacgacccc gactgccgca gaatgatgaa attcgaagct
    16621 tggaacaagc gtgagaagaa actttttgac aaaacagcca ctcttgccaa actacgcaat
    16681 accaatccgg tgttggttta cggagatttt ataaatttgc aggtcacgaa agatagctgg
    16741 atttttgccc ggaaatattt tgataaaaca gccattgtgt tgataaacaa cagcaatgag
    16801 tccaaagaga tggaagtgga aatcccctcg aatatacaag gaaatttcaa agccaatttc
    16861 ggaagtaaat ttacggtgaa aggtggaaaa ttaaaggtaa gtttagagcc gtttagtt
//
LOCUS       DAORKP010000033        16394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_237_length_16394_cov_8.255279, whole genome shotgun sequence.
ACCESSION   DAORKP010000033 DAORKP010000000
VERSION     DAORKP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_16394_cov_8.255279"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..2171)
                     /locus_tag="PLN75_03245"
     CDS             complement(111..2171)
                     /locus_tag="PLN75_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03166.1"
                     /translation="MKNTFYLILSALFIFGLFSCKSNTSGTENMKEKRFISEKSVSEV
                     TKSLLDSIGETQRIRIERGVKQVAQLWQQTDGTNEDFTTFCMENFIADSVQLDVLYKR
                     LEKNFETLSGYFNEIDVTLKEPIQLVGFEESPVDMLFGGYDVRAHMDDDFYANKIAFV
                     TALNFPFYSLDEKTKLGDTWTRKQWAYARMGDRFTSRIPAQVQQHLSQTLTNADSYIS
                     NYNIYMGQLVDAKGEKLFPADMKLITHWGLRDELKSDYADKVNGLNKQQLIYEVMKRI
                     IDQSIPSEVINNGKYTWNPVENKIFDSGKELPSKPENDVRYEVLLQNFKANRQIDAYS
                     PNFPTALSRNFDAAMEIPQKDVENLFKSLLSSQQVKEVAAYIKSRLGRDLQPFDIWYN
                     GFKAKGEISEDELTKITSAKYPTPLAVEKDLPNILIKLGWKSEKARQIASLVQVDASL
                     GAGHAWGAVLRGQKARLRTRIGENGMDYKGYNIAVHEFGHNTEQTITLNDVDYWMLNG
                     VPNNAFTEAVAFMFQKRDLELLGIKNANPDAEAFLALDNFWASYEIMGVALVDIAVWE
                     WMYANPNATPAQLKDAVITEAKKVWNEYYAGILGGKDEPILGIYSHMIDYPLYLSYYP
                     IGHLIDFQVEKQMKGKNMADEMQRMYTQGRIVPQIWMKNGVGQAISVEPTLKAVDEAL
                     KVVK"
     gene            complement(2389..2682)
                     /locus_tag="PLN75_03250"
     CDS             complement(2389..2682)
                     /locus_tag="PLN75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03167.1"
                     /translation="MKAVFITFDQSHKVDVIRILDKYLIRGFTFWDGTQGRGSGTGEP
                     HYGSHAWPSLNNSILTIVEDRQVKNLKESLTQLDEASPMLGLRVFVWGIDETF"
     gene            complement(2798..5911)
                     /locus_tag="PLN75_03255"
     CDS             complement(2798..5911)
                     /locus_tag="PLN75_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRG03168.1"
                     /translation="MKIYESAVKKPIMTILVFIGVIILGLFSLRNLSVDLLPKIDMNM
                     LMVITTYQGASAADVETNVTRPLENVLNTVPSLKELTSSSRENRSVVTMQFEFGKDLD
                     ELTNDVRDKLSLVKAMLPENAEEPIIMKFSTDMIPVIMLSATAEKSIPALYRILDDNV
                     ANRLARIDGVGSVSIAGAPQRQIQVYVDPVKMEAHKLTVEGIAQKIGAENINIPAGTM
                     DVGNETFSLRVQGEFKDPKEINNIIVGNYGGKSIFLSDVATVTDSFEERVQESYTNGV
                     QGAVMVVQKQSGANTVDIANQVLKALPEIQKNLPPDVKITVINDTSDGIKNTINGLTE
                     TVLFAFIFVIIVVLFFLGRWRATIIIILTIPVSLVASFIYLYATGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITTHIERGSRPKSAAIHGTNEVSLSVVASTLTLIAVFFPLTLV
                     TGFAGVMFKELGWIVTIIMTMSLICAMTLTPMLSSQMLRLDRNPTKLTQKIYSPIQRF
                     LDWLDNAYTRFVNWTVRNRKKTILFTTLFFILSLLPVGLIGTEFFPASDDGYISAKIE
                     LPIGTRMEITRELALELQKKWKEENPEIETISFSVGQASSSNVWGSLQSNGSHIIDMD
                     CRLVSIKNRDKSVFDLIAKFQAELDKMPEIRKSNVSTGENGMMGGQSNLEIDIFGYDF
                     NKTDQIARDLSTRFGKIEGLTNIQVSREEYVPEFQVDFDREKLAMHGLNIATASNFLK
                     NRINGLTASLYREDGQEYFIRISYDPRYRQSLTDVENILIYNTQGSPVRVKDLGTVVE
                     RFTPPTIQRKDRQRVVTVSATLSGTTIDKAVTAIKTEIDKVEIPSDIIIDIGGSYEEQ
                     QESFADLGILFFIIILLVYIVMASQFESLTYPFIIMFSVPFGISGVILAMVITGGTLN
                     IMSFIGLIMLVGIVVKNGIVLVDYINLNRERGMGIIRAVVAGGKSRLRPVLMTTATTI
                     LGMLPLALSQSEGSEMWRPMAVTVIGGLSVSTVLTLLVVPVLYTIAAGIGVKRQSKKM
                     AKKLQVK"
     gene            complement(5992..7035)
                     /locus_tag="PLN75_03260"
     CDS             complement(5992..7035)
                     /locus_tag="PLN75_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRG03169.1"
                     /translation="MKLNHLFLISLLALTVGLVSCGGKEKKTETVAEKPSVKLAKVFE
                     RNVPQIAEFTATIQPEVKNSIAPVTPGRIRRIMVEVGSQVGKGQRLVQMDAVNLENYQ
                     TQIGNLRENYNRLLELYQVGGVSKQDVDNLKAQLDQAETSMKNLQENTYLISPISGVV
                     TAKNYDNGDLFTGQLPVLTVMQINPLKVIVNVSETYFPKVRQGMGVDISFDAFPNQRF
                     GGTVSKIYPTVDEMTRTFGVEIKMQNSGNKVRPGMYARVTMNLGTENRVVVPDQAVVK
                     QVGSGNRFVYVYKDGKVSFNEVKLGQRLDAEYELISGVPAGSQVVVAGQSKLNDQMEV
                     TVSEEKKETEANK"
     gene            complement(7134..8465)
                     /locus_tag="PLN75_03265"
     CDS             complement(7134..8465)
                     /locus_tag="PLN75_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRG03170.1"
                     /translation="MKKTGLLIISLMLMSGLYSQDTLRLTLPQAVEIALSESPTIKIA
                     DKVIERTDYSKKSAWYALIPNLEGTGQYSKFLVPAKMNMFGQVMDSPSDFNASLGMSL
                     SLPLFAPALWHSIQMTTLEMQSAAEKAIASKITLRNEVTKAYYNVLVVQDSYKVLQDG
                     YALAKKNYEIAKKGYETGMLAAYDYISAEVQLNNLLPNILQAENGIAQSKAYLKVLMG
                     MDINIPIKVSDSLTDFESNIVEVKSINDYDFENNPDLRQLDIGRMQLQKSLQLQKTQR
                     MPTLAAFSQYGYSGTGSKQTMLNFGQVPIMVDASKDWFSQGLIVGLQLRVPITGIFTG
                     TIKEKQLKIQEQELLLQRDQAKSGIQLQAISALDKMNKSVKQVEAARKSVELSEKAYT
                     ISSKRYENGAGTMVELQNAALAITQSQLSYRQAISDYLSAKADLDKLIGNN"
     gene            complement(8493..8894)
                     /locus_tag="PLN75_03270"
     CDS             complement(8493..8894)
                     /locus_tag="PLN75_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03171.1"
                     /translation="MYRNKNAIDALILIIKEVKLHAHNDCTSFNYDLEKYYPSLYNDY
                     MKHKTDWMKESFMNNLRQGIEEGYYREDLDIELSAIYHVVQLSNSYRDILNHSPKIPV
                     KRITEFYIDMIVRLITNEKGLKYVQEQIMKD"
     gene            complement(9328..11733)
                     /locus_tag="PLN75_03275"
     CDS             complement(9328..11733)
                     /locus_tag="PLN75_03275"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRG03172.1"
                     /translation="MQNNIKSNSPSVSSSQNNGDTIHLRDLLFKYIQKWYWFVLVVII
                     CLILATLFIRKTETKYKIQTTILLRNDNTTPGISQMAMMESLGFNGVSKEVEDEIQVI
                     SSKKILKQAIDSLGLQIEYFEKDGLKYVEKYRDFPFTLNISKEILGTINQPLVFHVKE
                     SLGKYKLDMKYGHNFKEKYKLDNIQQTFETPLGKIGFISKNALEEGAKYKIVVYPINQ
                     LLEKNGGKISVSTVNKQSNAIQISTVESNIPKAQDYLNKIVELYNLDAIVDKNIIATN
                     TANFINDQLDIISKNLFKWETSVEGYKRSQSLTDLSSEATLYLESASEYEKKLTELGT
                     QLNMIQSVENYIKNPKNSGNLIPANVVTEDPALAKSIQEYNMAVLERMKLAQTAKEKN
                     PSLIQADQQIDALRANVLASVNSVKNGLQIAKNDVLKKDAQFNTRIKNVPTQERQFLE
                     IKREQEITQNLYIFLSQKKLENALSLASTAPAARTIDRAYASLDPVAPKRMTIYLVAI
                     MLGVLIPFLVIYLKDLINNKIEDSKEFQNLVKPPYLGNVAVSRESERVVVREGKTTPI
                     VEMFRLIRTNLQFLIGSKKSPVILITSSISGEGKSFTSINIAMSFALMKKKVVLIGMD
                     IRNPMLGEYMHIPKDKGVTLFITNTSYSINDVIEKSGFHPLLDVIPAGPIPPNPSELL
                     MSNRVDELIEELKKIYDYIIVDTAPIGVVSDTYLLNRIADNCIYVARQDYTPKDAADL
                     INEIYKENRLNGMGVILNGTPVSSGYGYSYGYGSKYKSSNAPKITFGDRLSDFIDKLT
                     KKN"
     gene            complement(11757..12551)
                     /locus_tag="PLN75_03280"
     CDS             complement(11757..12551)
                     /locus_tag="PLN75_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRG03173.1"
                     /translation="MKKNQIYFFTLMLILVIAGCTSQKKLTYFNGIDKNAADSINLRS
                     SFRHEAIIVSGDMLGITVSGLDPKAVANFNMPFVSYSSPGSDQIYAPQTLQPYVVDIE
                     GNIHFPVVGKIKAEGLTRSQLIALITKELEVYVEKPIVNIRFINSTVTILGEVNRPGQ
                     YALTNERSTLLDALGLAGDLTAYGKRENVLIRRENSGKVEFSRLNLNDGSLFNSPYYY
                     VQQNDVIYVEPNSVKSVSSQNIPLYLSSVSTLATLITLVYSISRSN"
     gene            12847..14205
                     /locus_tag="PLN75_03285"
     CDS             12847..14205
                     /locus_tag="PLN75_03285"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRG03174.1"
                     /translation="MEIKEYIKENENRFFEELFSLIRIPSVSSHSDKKAELMQCAEQW
                     KKLLLEAGVDKAEILPTKGNPVVFASKTTDPKLPTVMVYGHYDVMPAEPLELWDSPPF
                     EPEIRDGKVYARGADDDKGQSFLQAKAFEYLVKTNQLKCNVKFILEGEEEIGSPSLED
                     FCKKNKKLLACDTILVSDTSMLAADKPSITTGLRGLAYWEIEVTSANRDLHSGIFGGA
                     VANPINELAKILAQLTDNEGKITIPHFYDDVEKISKKERDLIRQIPFDEKEYMENLNI
                     NYLYGEKGYSTIERTGIRPTLDVCGIWGGYTGEGSKTVLPSKAYAKLSARLVPNQKYE
                     KIAKLVEKHLVKIAPKHIGIKVSRLHGAESYVCPIDSAEYIAAEKAYEKAFGKKPLPV
                     RRGGSIGVVPVFEKVLGVKPILMGFGLESNAIHSPNENFSIELYEKGMETIVEFYREY
                     CK"
     gene            14365..14622
                     /locus_tag="PLN75_03290"
     CDS             14365..14622
                     /locus_tag="PLN75_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRG03175.1"
                     /translation="MTKTITFNELRRIKDALPSGSMAKIANELGLQKETVKNYFGGTD
                     YKEGQSVGLHIEPGPDGGIVQLDDTSILEVAQRMLDEQRNK"
     gene            complement(14706..15647)
                     /gene="trxB"
                     /locus_tag="PLN75_03295"
     CDS             complement(14706..15647)
                     /gene="trxB"
                     /locus_tag="PLN75_03295"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRG03176.1"
                     /translation="MNEKVRCLIIGSGPAGFTAAIYASRANLSPVLYEGMQPGGQLTT
                     TTEVENFPGYPEGKTGPEIMEDLKKQAQRFGADIRYGMATAVDFTASPYKVTIDGDKI
                     IEAETVIISTGAEAKYLGIPDETKYAGFGVSACATCDGFFYRNRKVAVVGGGDTAAEE
                     ATYLSNIAEKVYLIIRKNYMRASKIMQDRVLANPKIEVLFEHETVGLTGERVVEGADL
                     VKRRGKPDEEKVHIDIDGFFLAIGHKPNSDIFKPYLETDEVGYIKVTPGTPRTAIPGV
                     FAAGDVADPHYRQAVTAAGTGCMAAIEAERYLSEKGL"
     gene            complement(15747..>16394)
                     /locus_tag="PLN75_03300"
     CDS             complement(15747..>16394)
                     /locus_tag="PLN75_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRG03177.1"
                     /translation="KMKDVKLIISLLIAWIIILPVAAQSNQNAVKVLNNTVSVLQKNA
                     IQTNFELTVKESASAKAQKMNGSFLMQGNKFVLNTSEMKVCFDGKTQWSYLPDVNEVS
                     ITNPTEKELAQTNPLALLQAYKNKSTIRFAAKNTVKAFHTIELLPKSKESDFQRIIVI
                     VNKSNSFPQSIQLTDKKGMNTLLTLTQFKTGIKTNNSTFTFITKNYKDIEINDLR"
BASE COUNT         4617 a         3372 c         3202 g         5203 t
ORIGIN      
        1 cgccatccat cttcattctc atgggtgaaa atttgtgttt aatggctgtt tcataaaatg
       61 aaacagaaat ggctgacatc atcctgtcag ccattttcat ttgcggaaat ctatttcacc
      121 actttcaacg cttcgtccac agctttcagt gtcggttcta cagatatggc ctgtcctact
      181 ccgtttttca tccagatttg cggcactatg cgtccctgcg tgtacatgcg ctgcatttcg
      241 tcggccatgt tttttccttt catctgcttt tctacctgaa aatcaatgag atgaccaatg
      301 gggtaatacg aaaggtaaag cggatagtca atcatgtgtg aatagattcc gagaatcggt
      361 tcgtcttttc cacccaaaat gccggcatag tattcgttcc agactttttt ggcttcggtg
      421 attaccgcgt ctttcagctg agcaggagtg gcattaggat tggcatacat ccattcccaa
      481 acggcaatat caaccaatgc tacacccata atttcataac ttgcccagaa attatcgagt
      541 gcaagaaatg cttcggcatc cggatttgca ttttttatcc ccagtaactc caggtcgcgt
      601 ttttggaaca tgaatgcaac cgcttcggta aatgcattgt tgggtactcc gttgagcatc
      661 cagtaatcca cgtcgttgag tgtaatggtt tgttcggtat tgtgtccgaa ttcatgtacg
      721 gcgatgttgt agcctttgta atccattccg ttttcaccaa tgcgggtacg caggcgggct
      781 ttctgaccgc ggagcacggc tccccatgca tgtccggcgc caagcgatgc atcaacctgt
      841 accagcgaag ctatttgtcg ggctttttcg gatttccatc cgagtttgat caggatattt
      901 ggcagatctt tttcaacagc cagcggtgtg gggtattttg ctgatgttat tttagtcagt
      961 tcatcttcgg atatttctcc tttagcctta aagccgttgt accagatatc gaagggttgt
     1021 aaatctcttc caagccggga tttgatgtat gcagccacct ctttcacctg ctgagatgaa
     1081 agcagcgatt tgaatagatt ttcgacgtct ttttgaggta tttccatggc agcatcaaaa
     1141 ttacggctca aagcagtcgg gaaatttggt gaataggcat caatttgtcg gtttgccttg
     1201 aaattttgga gtagaacctc gtaacgaaca tcgttttcag gttttgaagg caattctttt
     1261 cccgaatcaa aaattttatt ttcaacggga ttccatgtgt attttccatt gttgattacc
     1321 tccgaaggga tggactggtc gataatacgt ttcatcactt catagataag ctgctgttta
     1381 ttaagtccgt tcaccttgtc ggcgtagtcc gatttcagtt catcgcgcag tccccagtga
     1441 gtaatgagtt tcatatcagc cggaaaaagt ttttcgcctt ttgcatccac aagctgcccc
     1501 atgtatatgt tgtaattaga gatataggaa tcggcattgg tcaatgtctg agacaggtgc
     1561 tgctgaacct gcgccggaat acgcgatgta aaacggtcgc ccattcgggc ataagcccat
     1621 tgcttacgtg tccaggtgtc acccagtttg gttttctcat ccagtgagta aaacggaaaa
     1681 ttcaaggcag taacaaaagc aattttattg gcataaaagt cgtcgtccat gtgtgctctc
     1741 acatcatagc caccaaaaag catgtcaacg ggagattcct caaaaccaac aagctgaata
     1801 ggttctttga gcgttacatc tatttcgttg aaatagccgc tgagcgtctc aaaatttttc
     1861 tccagccgtt tgtacagcac atccaactga acggaatctg caatgaaatt ttccatgcag
     1921 aatgttgtaa aatcttcgtt tgtgccatcg gtttgctgcc agagttgagc aacctgcttt
     1981 acgcctcgct caattcgtat tcgttgtgtt tcacctatcg aatcaagcaa tgattttgta
     2041 acttccgaaa ccgatttttc ggaaataaac cgcttttctt tcatattttc agtacccgaa
     2101 gtatttgatt tacagctaaa aagtccgaaa atgaacaatg ctgataaaat aagataaaat
     2161 gtgtttttca tattcaaaaa taattatacg attcatagat tatacaaaga taatggaaat
     2221 aaatctgaag gaatgaatga agcgcaaaaa gttaataaat ttacgataat gaattagtct
     2281 gtaaattggt ttttttacat ttctgacgaa cataaagagg aactaaaaat caagaaaaga
     2341 cgaatataat atccgtcttt tcttactcta tggttaaaaa aaatgcattt aaaacgtttc
     2401 atcgataccc cagacaaata cgcgcaaacc gagcattggt gaggcttcat ccaattgggt
     2461 cagactttct ttcaggtttt tcacctgcct gtcttccact attgtaagaa ttgaattatt
     2521 aagcgatggc caggcatggc ttccgtagtg aggttcacct gtaccggagc cgcgtccctg
     2581 tgttccatcc cagaatgtaa atccacgaat taaatactta tccaatatcc tgatcacatc
     2641 gactttgtgc gactgatcaa aagttataaa aactgctttc ataatttatt gtgtacaact
     2701 tcaaaaacct gcttcattaa gtagattcag aaatatttga ttttgatttt gaaaaaatta
     2761 ttttttcaca aataaaagag attgaagact gcaagcctta ttttacctgt aatttctttg
     2821 ccatcttttt cgactgtctc ttcacgccaa ttcctgctgc aatagtgtaa agtacgggta
     2881 ctacaagtaa tgttaatacc gtggatacag acaaaccgcc tattacagta acggccatcg
     2941 gacgccacat ttcagaaccc tcgctttgcg aaagggcaag gggaagcata cccagaatgg
     3001 tagtggcagt tgtcatcaac accggtctta aacgtgactt tcctccggct accaccgccc
     3061 ggataattcc cattccacgt tcacggttca gattgatgta atccaccagt acaataccgt
     3121 ttttaaccac aatacccacc aacatgataa gcccgataaa cgacatgata ttcagcgttc
     3181 ctcctgtaat aaccatggcc agaattaccc ccgaaatacc aaaaggtaca gagaacatga
     3241 tgataaacgg atacgtgagt gattcaaact gcgatgccat gacgatgtaa accagcaata
     3301 taatgatgaa gaagagaatt ccaagatcag caaacgactc ctgctgctct tcgtacgagc
     3361 caccgatatc aataataata tccgaaggta tttccacctt atctatctca gttttgatgg
     3421 ctgttactgc cttgtcgatg gtggtgcctg aaagtgtagc cgaaacagtt accacacgct
     3481 ggcggtcttt acgttgtatg gtcggtggtg taaaccgttc aactacagtt cccaaatcct
     3541 taacccgtac cggcgatccc tgtgtattat aaataagtat gttttcaaca tcagttaagg
     3601 actggcgata tcggggatcg taagaaatac gtatgaagta ctcctgacca tcttcacgat
     3661 aaagagatgc tgtaagtccg ttgatacgat ttttaagaaa attggaggct gtagcaatat
     3721 ttaatccatg cattgcgagc ttttctctgt caaaatccac ctgaaactca ggtacatatt
     3781 cttcacgact tacctgtata ttagttaatc cttcaatctt accgaaacgt gtactcaggt
     3841 ccctggctat ttggtcggtt ttattaaaat catatccaaa aatatctatt tcaagatttg
     3901 actgtccgcc catcatgccg ttttcaccgg tggatacgtt tgatttcctg atttcaggca
     3961 ttttatccag ttctgcctga aatttggcaa tcagatcaaa taccgattta tcacggttct
     4021 taatacttac cagtcgacaa tccatatcga taatgtggct tccgttgctc tgcaacgatc
     4081 cccaaacgtt cgaactgctt gcctgtccta ccgagaaact gatagtttct atttcaggat
     4141 tttcttcttt ccatttcttt tgaagttcca gggcaagctc gcgtgttatt tccatgcgtg
     4201 tacctatcgg aagttcaatt tttgcagaaa tatacccgtc gtccgatgca ggaaagaact
     4261 cagttccgat taatccaacc ggaagtaaac ttaaaataaa gaacagagtg gtaaataata
     4321 tggttttctt gcggttacgt acggtccagt ttacgaatcg tgtataagca ttatccagcc
     4381 aatccagaaa acgctgaatg ggtgagtata ttttttgtgt aagttttgta ggatttctgt
     4441 ccagccggag catttgtgaa ctcagcatgg gtgtaagcgt catggcgcaa atcaacgaca
     4501 tggtcatgat gatggtaaca atccagccca attccttaaa cataactccg gcaaaacctg
     4561 taaccagcgt gagtgggaaa aacacagcaa tcagcgtgag tgtagatgcc accactgaaa
     4621 gcgaaacctc gttggttccg tgtatagccg cacttttggg gcggcttccc cgttcgatat
     4681 gtgtggtaat attctcgagc accacgatgg catcgtccac caccatcccg atggctatgg
     4741 acaaggagct tagtgagatg atattgaggg tgccgcccgt agcgtataga tatataaatg
     4801 aggcaacaag cgataccgga atggtaagaa tgataatgat agtagccctc cagcgcccta
     4861 agaagaataa tactacgatt attacaaata tgaatgcaaa aagaaccgtt tcggtcagac
     4921 catttattgt attcttgata ccgtcagagg tgtcatttat taccgtgatt tttacatcgg
     4981 gtggtaagtt tttttgtatt tccggcaagg ctttgaggac ctggttggca atatctacgg
     5041 tgtttgctcc ggattgcttc tgaactacca ttaccgcacc ctgaacgccg ttggtataac
     5101 tttcctgaac gcgttcttca aaactatccg ttacggtagc aacgtctgaa agaaaaatgg
     5161 attttccacc ataattacct actatgatgt tgtttatttc tttgggatct ttgaattctc
     5221 cctgtacacg cagcgaaaat gtttcgttac ctacatccat tgtaccggcc ggaatattga
     5281 tattttctgc accgattttc tgtgcaatgc cttcaacagt gagcttgtga gcttccattt
     5341 tcaccggatc aacataaacc tgaatctgac gttgaggagc accagctatg gatactgaac
     5401 ctactccatc aatacgtgcc agacgattgg caacattgtc gtccagaatc cggtataatg
     5461 ccggaatgct tttttcggca gtagctgata gcatgataac cggtatcata tctgtgctaa
     5521 acttcatgat aatcggctct tcggcgtttt cgggcagcat ggcttttacc agactgagtt
     5581 tatcacgcac atcgttagtg agttcgtcca gatcttttcc gaattcaaac tgcatcgtca
     5641 ctaccgaacg gttttctctc gatgacgagg tgagttcttt cagactggga acagtattca
     5701 aaacattctc aagcggacgt gtcacgttgg tttctacatc ggctgcactg gcgccctggt
     5761 aagttgtaat taccatcagc atgttcatat caatttttgg cagcagatcg accgacagat
     5821 ttctcaacga aaaaagtccc agaatgataa caccaatgaa gaccaggata gtcataattg
     5881 gcttctttac agcactttcg tatattttca tttaatgtgt ttttttactt ttttcactct
     5941 ccgtaaagtg agatttattt tttctaaaaa aaagcgacga aatgaacgat cttatttatt
     6001 ggcttctgtt tctttctttt cttcgcttac ggttacctcc atctgatcgt tgagctttga
     6061 ctgacctgct acaaccacct gcgaaccggc aggaacacct gaaataagct catattcggc
     6121 atcaaggcgt tgcccaagtt ttacctcatt gaatgacact tttccatcct tgtacacata
     6181 cacaaaccgg ttacccgaac ctacctgttt taccacagcc tgatcgggca ctactacgcg
     6241 gttttcagta cccagattca tggttacacg tgcgtacata ccgggacgaa ccttgtttcc
     6301 gctgttttgc atttttatct caacgccaaa agtacgggtc atttcatcca ccgtagggta
     6361 tattttactt accgttccgc cgaatcgctg attggggaaa gcatcaaaac tgatatccac
     6421 tcccataccc tgtctaactt tggggaaata ggtttcggat acattcacta ttactttcag
     6481 tggattgatc tgcatgacgg taagtaccgg aagctgaccg gtaaacaaat caccgttgtc
     6541 gtaatttttg gctgttacca ctccgcttat cggactgatg agataggtat tctcctgtaa
     6601 attcttcatc gaagtttcgg cctgatccag ctgagctttc agattgtcaa catcctgttt
     6661 tgatacacct cctacctgat acaattcgag caggcggttg taattttcac gcaaattacc
     6721 aatctgagtt tgataattct ccagatttac agcatccatt tgtaccaaac gctgaccttt
     6781 gcccacctgt gaacccactt caaccatgat gcggcgtatg cgtccaggtg ttacaggtgc
     6841 aatgctgttt ttcacttcgg gctgaatggt agctgtaaat tcggctattt gcggtacgtt
     6901 gcgttcaaat acctttgcaa gttttactga tggtttttcg gccacagttt cagttttttt
     6961 ctccttgccg ccacacgaaa cgaggcccac tgtcagtgca agtaaactta ttaaaaacaa
     7021 atgattaagt ttcatatatt ttttgttgtt attaaattct attattttaa aattcgccct
     7081 gattcgggta tttatattat taaaaatttg attttactat aatctgatgc cttttaatta
     7141 tttccgatca gtttatccag atcggctttt gctgataaat aatcagaaat tgcctgacga
     7201 tatgataatt gtgactgagt aatagctaaa gcggcgtttt gcagttctac catggttccg
     7261 gcaccgtttt cgtatctttt tgaggaaatg gtgtatgcct tttcggataa ttccacactt
     7321 tttcgggctg cttcaacctg ttttaccgat ttattcattt tgtcaagtgc tgagattgcc
     7381 tgtaattgta ttccgctttt ggcctggtcg cgctgcaaca gcagttcctg ttcctgaatt
     7441 ttaagctgtt tttctttgat tgtaccggtg aaaatgccgg ttatgggtac ccgcagctga
     7501 agtcctacga tcagtccctg gctaaaccag tctttgcttg catctaccat aatgggcact
     7561 tgtccaaaat tcaacatggt ttgtttgctt ccggttccgc tatatccata ttgactgaaa
     7621 gcggcaagag tgggcattcg ttgagttttt tgcaattgaa gtgatttttg gagctgcata
     7681 cgaccaatat caagctgacg taagtcggga ttattttcaa aatcataatc atttatgctt
     7741 tttacctcca caatgttgct ttcaaaatcg gtgagtgaat ccgatacttt tatcggtatg
     7801 ttgatatcca ttcccatcag tactttcaga taggctttgg actgtgctat accgttttcg
     7861 gcctgaagta tgttggggag cagattgttc agctgaactt cggccgaaat gtaatcgtaa
     7921 gcagcaagca taccggtttc ataccctttt ttggcaattt catagttttt ctttgccagt
     7981 gcgtagccat cctgcaacac tttgtatgag tcctgtacta ccagtacgtt gtagtaggct
     8041 tttgttactt cgtttctgag tgtaattttc gaagcaatgg ctttttcggc agctgattgc
     8101 atctcgagtg ttgtcatctg tatgctgtgc cacagagctg gtgcaaagag tggcagcgag
     8161 agtgacattc cgagcgaagc attgaaatcg gaaggtgaat ccattacctg accaaacata
     8221 ttcatctttg caggaacaag aaatttggag tattgtcccg ttccttccag attaggtatc
     8281 agagcgtacc aggcactctt cttggaataa tctgtccttt cgattacctt atcggcgatt
     8341 ttaatcgtcg gactttcact cagagctatt tctacagcct gaggcagagt caaccgcagg
     8401 gtatcctgcg aatagagacc gctcatcagc atcaggctta ttattaataa tccggttttc
     8461 ttcatttttt tttgatgtaa aaaattcttt ttttaatctt tcattatttg ttcctgaacg
     8521 tatttcaatc ctttttcgtt tgtaatcagt cttactatca tatcgatgta aaattccgtg
     8581 attcgtttaa ccggaatttt cggtgaatga ttcaaaatat ctctgtatga attgcttaac
     8641 tgaacaacat gatatatggc gcttaattct atatctaaat cctctctgta atatccctct
     8701 tcgattccct gcctgagatt gttcataaaa ctttctttca tccagtcggt tttgtgtttc
     8761 atgtaatcat tgtataagga aggatagtac ttttcaagat cgtaattgaa agaagtacaa
     8821 tcgttgtgag catgcaattt cacttctttt attattaata tcaacgcatc aatggcattt
     8881 ttgttccggt acattttcat gaacttttca gtcactaaag acgtttgaaa ttcgagaaca
     8941 gctttaacca aatcttcctt ttttggaaaa atctgataaa atgttttttt tgaaaagcgt
     9001 aactcgttac aaatatcatc aatagaaata ctcctaatgc caaattgctg aaacttggct
     9061 gaagcagagt gaatgatttg ttgagtaagt tgatccataa accggggaaa aaattcaatt
     9121 gcaaaagtaa aaaataatat caataaattc gaataattac acattatttt ttgttaagaa
     9181 aaatttcaat catttcatat tgctgtttat caactattta ataatagtac ttggaaactt
     9241 ataaattttc atggtttcct aatccaaaat taaaaattaa aaaaacctca gatgaaagcc
     9301 tgaggttttt gatttataaa aatattatta attctttttc gtcagcttat ctataaaatc
     9361 tgatagtcta tcaccaaaag taattttcgg ggcattcgaa gatttgtatt tactgccgta
     9421 accgtaactg tagccatatc ctgatgatac aggtgtgcca ttcaggatta cccccatgcc
     9481 gttcaacctg ttttccttat atatttcatt tatcagatct gcagcatctt ttggagtata
     9541 atcctgacgt gctacgtaaa tacagttgtc agcaattctg ttgagcaaat aagtgtcaga
     9601 aaccacacca atgggagctg tgtccacaat aatgtaatca tagatttttt tcaattcctc
     9661 tattaattca tccacgcggt tgctcatcag cagttctgat ggattgggtg gtatgggtcc
     9721 tgccggtatc acatcaagaa gtggatgaaa tcctgatttt tcgattacat cgtttatgct
     9781 gtaactggta ttagttataa aaagtgtcac tcctttgtct ttcggtatgt gcatgtattc
     9841 acccagcatt ggattacgta tgtccattcc tatgagcaca actttctttt tcatcagtgc
     9901 aaaagacata gctatattta tggaagtgaa agatttacct tcgccgctta tgctggaggt
     9961 tatgagaatt accggtgact ttttggagcc tatcaggaat tgaagattgg ttcggatgag
    10021 acgaaacatt tcaacaatcg gagtggtctt tccttctctc accaccaccc tttcggattc
    10081 gcggcttact gcaacattgc ccaaatacgg tggtttcacc aaattctgga attcttttga
    10141 gtcttctatc ttattgttga tcaaatcttt aagataaata accaagaacg gaataagtac
    10201 acctaacatg atggctacca ggtaaatggt cattcttttg ggtgctaccg gatcaagtga
    10261 tgcataagct ctgtcaatgg tacgggctgc gggcgctgta ctggcgagac tcaatgcatt
    10321 ctcaagtttc ttctgtgaca gaaaaatgta caggttctga gtaatttcct gttcccgttt
    10381 tatttccaga aattgacgtt cctgtgtcgg aacattctta atccgggtat tgaattgggc
    10441 gtctttcttg agaacatcat ttttagctat ctgaaggccg tttttcacac tgttgacact
    10501 tgcgagtaca tttgcccgaa gggcgtctat ctgttgatct gcctggataa gtgagggatt
    10561 tttttctttt gccgtttgag cgagtttcat cctttcgagt acggccatat tatattcctg
    10621 aatagattta gctaatgcag gatcttcggt aactacgttg gcaggtatta aatttccaga
    10681 gttcttagga tttttaatgt aattttcaac cgattgaatc atattaagct gtgtgcccag
    10741 ttcggtgagt ttcttctcat attcgctggc agactcaaga tacagagtag cttcggatga
    10801 aaggtctgta aggctttgtg aacgtttata cccctcaacg cttgtttccc atttgaataa
    10861 attttttgaa ataatatcta actggtcgtt gatgaagttg gctgtattgg tagctatgat
    10921 gtttttatca acgatggcat cgaggttgta taattcaact attttattta gatagtcctg
    10981 tgctttggga atattggatt caactgttga aatctgaatg gcgtttgatt gcttgtttac
    11041 ggtggataca ctaatttttc caccattttt ctcgagcaac tggttaatgg gataaaccac
    11101 aattttatac tttgcacctt cttccaacgc atttttggaa ataaaaccaa tcttacccag
    11161 tggagtttca aaagtttgct gaatattatc caacttatat ttttctttga aattatgacc
    11221 gtatttcata tcaagtttgt atttaccaag actttctttg acatgaaaaa caagcggttg
    11281 atttattgtt ccgagaattt cttttgaaat atttagtgta aatggaaaat ccctgtactt
    11341 ttccacatat ttcaatccat ctttttcaaa atactctatt tgtagtccta atgaatcaat
    11401 tgcctgtttg agtatttttt tcgaactaat tacctgaatt tcatcttcca cttctttgga
    11461 aactccgttg aaacccagac tttccatcat ggccatttga gaaatgccgg gcgtggtgtt
    11521 atcatttctg agaagtatgg tagtttgaat tttgtatttg gtttcggttt ttctgataaa
    11581 aagcgttgcc agaatcaggc aaattattac caccagtaca aaccagtacc acttttgaat
    11641 atacttgaac agcaaatccc ttaaatgaat tgtgtctcca ttattctgag aactgctgac
    11701 tgagggtgaa ttcgatttta tattgttttg cataacgaac cgtctgagat tttaatttag
    11761 ttggatctgg aaatagaata taccagcgtt attaatgtag ccaaagtgga tacagatgac
    11821 aaataaagag gtatattctg tgatgaaact gattttacgc tattaggttc tacgtaaata
    11881 acatcgtttt gctgaacgta gtaataaggt gaattaaata aacttccgtc gttgagattt
    11941 agtcttgaaa actccacttt tccactgttc tcgcgtctta tcagtacgtt ttcccgtttt
    12001 ccgtaggccg tcagatcacc cgccagcccc aacgcatcca gcagcgtact gcgttcgttt
    12061 gtcagcgcat actgaccagg tctgttcacc tcaccgagta tagtaaccgt agaatttata
    12121 aaacggatat ttacaatggg cttttccaca tacacttcca actctttagt aatcaaagca
    12181 ataagctgac ttctggtaag accttcggct tttatttttc ccactaccgg aaaatgaata
    12241 tttccttcaa tatctaccac gtagggttgc agagtttgag gagcgtaaat ctggtcggat
    12301 ccgggtgatg aataggatac aaacggcatg ttgaaattgg caacagcttt cgggtccagg
    12361 cccgaaacgg taatgcccag catatcacca ctgacaatta ttgcttcgtg gcggaacgac
    12421 gaccggagat ttatcgaatc tgctgcattt ttatctattc cattgaagta tgtcagtttc
    12481 ttctgacttg tacaaccggc tatcaccaat attaacatca atgtaaaaaa ataaatttgg
    12541 tttttcttca tataaaatct tgtatataat ttttcgaaaa atatcaatac caaacgtaaa
    12601 aattatgaac ttattggcta cttaaataaa ataacttaca aaaataggaa aaaaatgcat
    12661 gagtttactc aaaataaact attaaaatca aaagttttcg tttttcaaga aataaacatt
    12721 tgataccgca aaagtattta aagaaggttt gtttaagaat gaatttaatt tttcatgatt
    12781 aaaaaccttt ctcgtcatca aaacaaatgc attcgttttt cgttaaatta taaaacacaa
    12841 tttatcatgg aaataaaaga atacatcaaa gaaaatgaaa accgtttttt cgaggaactt
    12901 ttcagtctga tcagaatacc cagtgtgagt tcgcattccg acaagaaggc cgaattaatg
    12961 caatgtgccg aacaatggaa aaaactgttg ctcgaagccg gagttgataa agccgaaata
    13021 ctccctacaa aaggcaatcc ggtagttttt gcatcgaaaa ctaccgaccc gaaactacct
    13081 acagtgatgg tttacggcca ctacgacgtg atgccggcag agccgttgga gctttgggat
    13141 tcgccgccat tcgaacccga aatacgcgac gggaaagtat atgcgcgggg agccgacgat
    13201 gataaaggac aatcgtttct gcaggccaaa gcattcgaat acctggtaaa aaccaaccag
    13261 ctgaaatgca atgtgaaatt catcctcgaa ggcgaagaag aaatcggatc gcccagcctg
    13321 gaagatttct gcaaaaaaaa caaaaaatta ctggcctgcg ataccatact tgtatcggat
    13381 acaagcatgc tggcagccga caaaccaagt atcacaaccg gactgagagg gctggcatac
    13441 tgggaaattg aagttacatc ggccaacagg gatcttcatt cgggtatttt cggcggagct
    13501 gtagccaatc ctatcaacga acttgcaaaa atcctggcac aactgactga taatgaagga
    13561 aaaattacga taccgcattt ctatgacgat gtggagaaaa ttagtaaaaa agaacgggat
    13621 ttaatccgcc agattccttt tgacgaaaaa gaatacatgg aaaacctcaa tatcaactat
    13681 ttatatggtg agaaaggtta ctcaaccatc gagcgaaccg ggatcaggcc tacgctggac
    13741 gtatgcggaa tatggggtgg ctatacgggc gaaggcagca agaccgttct tccctccaaa
    13801 gcctacgcca agctttcagc acggctggta cccaatcaga aatatgaaaa gatagccaaa
    13861 ctggtagaga aacatcttgt aaaaatagct ccaaaacaca tcggtataaa agtcagcagg
    13921 cttcacgggg ctgaaagcta tgtatgtccc attgattcag cagaatacat tgcagctgaa
    13981 aaagcatacg aaaaagcatt tggtaaaaaa ccattgcccg tacgccgagg cgggagcatc
    14041 ggagttgtgc cggtatttga gaaagtgctg ggtgtgaaac cgattctgat gggattcggg
    14101 ctggaatcca atgccattca ctcaccgaat gaaaatttca gcattgaact gtatgaaaaa
    14161 ggcatggaaa ctattgtaga attttacagg gaatactgta aataattaat atactgaatt
    14221 tcaattaaat agttacaaaa tgaaattatt tttcagcatt ttttgttttt ttgcacaaaa
    14281 atgttttgta aaacaaaaaa aatgtactat gtttaacatt caatattgag ttcaaattta
    14341 ttaactataa aaacattttt tgccatgact aaaacaataa cattcaacga attacgtaga
    14401 ataaaagatg cactgccaag cgggagtatg gccaagatcg ctaacgaact tggtctgcaa
    14461 aaagaaacag taaaaaacta ttttggcgga acagactaca aagagggtca aagtgtggga
    14521 ttgcatattg aaccgggacc tgacggaggg attgttcaac tggatgacac aagcattctg
    14581 gaagttgctc agcgtatgtt ggatgaacaa agaaacaaat aaatcaaacc gaaagaagca
    14641 gtgaaattca ctgcttcttt tttatttaca actttcattt atttcaataa aaaattgcaa
    14701 aaattttaca atcctttttc tgacaaatat ctttcagctt cgatagctgc catgcagcca
    14761 gtacctgcag cagtaactgc ctggcggtaa tgaggatcag ccacatcacc cgctgcaaat
    14821 acacccggta ttgccgtacg gggagtgccc ggagttacct ttatgtaacc cacttcatcg
    14881 gtttcaagat agggtttgaa aatatctgaa ttgggcttat gaccaatggc cagaaaaaat
    14941 ccatcgatgt cgatatgtac tttttcttca tcgggtttac cgcggcgttt cactaaatca
    15001 gcaccttcca ctaccctttc gcctgtcaga cctacggttt catgctcaaa aagcacttca
    15061 attttcggat tggcaagcac tctgtcctgc atgattttag atgcacgcat gtagttttta
    15121 cgaatgatca aatatacttt ttcggcaata ttggataagt aggtagcctc ttcggcagcc
    15181 gtatcaccgc cacccactac agctactttt cggttgcgat agaagaaacc atcgcacgtg
    15241 gcacacgccg aaactccaaa accggcatat ttcgtttcat ccggtatgcc caggtatttt
    15301 gcttcggcac cggtagatat gattactgtt tcagcttcga taattttgtc tccatcgatc
    15361 gtcactttat aaggtgatgc tgtaaaatca acagccgtag ccattccgta tcgtatatct
    15421 gcaccgaaac gctgggcttg ttttttcaaa tcttccatga tttcgggtcc ggtttttcct
    15481 tcaggataac ccggaaaatt ttcaacttcg gtagtagtgg tgagctgccc acccggctgc
    15541 attccttcat acaatacagg tgataaattg gcacgcgaag cgtatatggc agcagtaaaa
    15601 cctgccggtc ctgaacctat tatcaggcat cttacttttt cattcatata tgtttatttt
    15661 tttagtataa taatttttaa aacaaagcgt gcatcaatcc ccgatttgaa agattaatgc
    15721 actgcaaaga tatagctttt tattttttat ctcaaatcat taatttctat atctttataa
    15781 ttttttgtga taaatgtaaa tgtactgttg tttgttttta ttcctgtttt gaactgagtg
    15841 agggtaagca aggtattcat tcctttttta tctgtaagct gtatagattg aggaaaggag
    15901 ttggatttat tcactatcac aattattctc tgaaaatcag attcttttga tttgggcagc
    15961 aattcaatgg tatgaaaagc cttgactgta tttttggctg caaaacggat agttgatttg
    16021 tttttgtatg cctgaagcaa tgccagcgga ttggtttgtg ccagctcctt ttcggtagga
    16081 ttggtaattg atacttcatt cacgtcaggc aggtaactcc attgagtttt cccatcgaaa
    16141 cagactttca tttcactcgt gttcagcaca aatttatttc cctgcatcag aaacgagccg
    16201 ttcattttct gcgctttagc cgatgcggat tcttttacgg tgagttcaaa gtttgtttga
    16261 atggcatttt tctgcaatac actcacggta ttattcaaaa ctttgacggc attttgattg
    16321 ctctgagctg ctacgggcaa aatgattatc catgcaatca acaggctgat tattaatttt
    16381 acatctttca tttt
//
LOCUS       DAORKP010000034        15788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_260_length_15788_cov_8.810144, whole genome shotgun sequence.
ACCESSION   DAORKP010000034 DAORKP010000000
VERSION     DAORKP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_15788_cov_8.810144"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..869
                     /gene="hisF"
                     /locus_tag="PLN75_03305"
     CDS             114..869
                     /gene="hisF"
                     /locus_tag="PLN75_03305"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRG03178.1"
                     /translation="MLTKRIIPCLDVKDGKTVKGINFVDIRDVGDPVELGKRYAEMGA
                     DELVFLDITATHEKRKTLSELVTHIAREINIPFTVGGGISSVEDVSLLLSCGADKISV
                     NTAAVRNPQLIADLADNFGSQCVVLAIDTKCIDGEWYVFLNGGRIPTEIKTMDWARQA
                     VALGVGEILLTSMNHDGTKDGFATDITRAVSEAVNVPVIASGGAGTMQHFVDVFNQGK
                     ADAALAASIFHYEEIGIAELKHYLKEKGIAVRI"
     gene            complement(904..2760)
                     /locus_tag="PLN75_03310"
     CDS             complement(904..2760)
                     /locus_tag="PLN75_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG03179.1"
                     /translation="MSNAKLSASEIVELMVKDRMITKKSAEEFVKVFFSVIEDALKDG
                     ETVKIKGLGTFKSQWNEARKSVNVNTGEEIVIDGYYKAVFIPENDLRELINEPFSHLE
                     PVELNPLSDNLPEQMQDDAEEDEPENFSEPLEPLRVFGEQAEEIKELLSEINSFSTPK
                     VRSNDTVTENESSQLPDPDEKTEEEITLENTPMDKPESVSEVAEIISAPETLHTPEHT
                     GNKETEFNFDGFDIVREISTRFGLPAGAAAVALINEESDQLPAEAESETESNISEVLP
                     EEIPENTVAAEKHEIDDREAEDDNNDDEKQEKFEEIDDDDETDENEADDETDEESDED
                     EADDETDEDEQLIPEDDTEAEDGDDNDEDTDETVVELPEVVTQTDEEAAETIEADNTA
                     AKEKSVEPLVAVKNARTVTDKKKIVSTSSSHKRRRHYHVEEKKKRPVWLYTLLGIAAL
                     GLVFFGAFYFVKNILDKQKDAMYMEYLADSTVRALNTKEVLDSMTPAGDSIATTDTVA
                     VKATDESVQTVISEAPAAGNIFDAPRNYTELITTEKMVAGSQLTKFARKYYGHPHFWV
                     YIYEANREAIKDPNNVPLGINIKIPKLDARLADPASRECLNYALKLQSEYLK"
     gene            complement(2765..3046)
                     /locus_tag="PLN75_03315"
     CDS             complement(2765..3046)
                     /locus_tag="PLN75_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG03180.1"
                     /translation="MNNKELISNVASKMNLPKNEVKYLLEAFTETFRNQLKEDNSIGF
                     QGFGSFEIRKKEERLSVHPLTKVRTMIPPKLVVSFKQSSVLKDKLNVKF"
     gene            complement(3100..4389)
                     /gene="rimO"
                     /locus_tag="PLN75_03320"
     CDS             complement(3100..4389)
                     /gene="rimO"
                     /locus_tag="PLN75_03320"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRG03181.1"
                     /translation="MKKNTVDIITLGCSKNLVDSEQLMRQFDVLGYNVRHDAENPDGE
                     IVIVNTCGFIGDAKEESINMILQLAERKRELKNKLFVMGCLSERYLHDLETEIPEVDK
                     FYGKFNYVDILKEIGQEYRADLKLERTLTTPLHYAYIKISEGCNRTCSYCAIPIITGR
                     HRSRPIEDIEKEVKLLVEKGVKEFQLIAQDLTYYGIDRYKKSKLAELVERLSDIQGVG
                     WIRLHYAYPAHFPYDVLRVMRERENVCKYLDIALQHSSDHMLGVMRRNFTSAQTYELI
                     DKIRSEVPGIHLRTTMLVGHPEETEQDFEELKEFVRWARFERLGAFAYSHEEGTYAHK
                     NYTDNIPDETKQQRLGELMDIQQRIAAEINAAKTGSILKVIIDRKEGDYFIGRTEFDS
                     PEVDPEVMIPADTKGVRVGEFYQATIVDATDFELFGE"
     gene            complement(4587..6578)
                     /locus_tag="PLN75_03325"
     CDS             complement(4587..6578)
                     /locus_tag="PLN75_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HRG03182.1"
                     /translation="MRNLLTFILICATTLAFSQKQFVLLSPDGKLKTEISVGKTTEFA
                     ITHNGDLMLEKSPVSMKLSEGKIPGPDAKVKKAVKKTVNEIIDAKIYKKSKVENNYNE
                     LNILFSGDYSLIFRAYDDGVAYRFETNFKKPYTVENEEAKFNFPDDPKAFLAYVNKSD
                     NTTFSQQFYNSFENTYHHIPLKEANPRLLAFLPLLVEGKNGKNVCITEADLLNYPGMY
                     LYNPDGTSSLTAMFAPYPREIRQGGHNMLQGEVQSAENFIARCEGKTTFPWRTVIIAE
                     NDYELTDNDMVYKLATPNQLADISWIKPGKVAWDWWNDWNIRNVDFEAGINNETYKYY
                     IDFAAEQGIEYVILDEGWAVNKKADLFQVIPEINLTELVEYGKSKKVDLILWAGYWAF
                     NRDIEGICKHYAEMGIKGFKVDFMDRDDQQMVDFYHRAAQIAAKYNLMLDFHGAYKPT
                     GLQRTYPNVINFEGVHGLEQMKWSPASVDQVTYDVTIPFIRMVAGPMDYTQGSMRNAT
                     RNNYRPVYSEPMSQGTRCRQLAQYVIFEAPFNMLCDNPSNYLAEPECTQFIAEIPTVW
                     DETVALNGEISKYVTIARRTGNEWYLGSMTNWDSRTLDVDLSFLGDGNFKAEIFRDGV
                     NAHRTAMDYRKETVDVSADRKMTLKMAPGGGFAMKITKL"
     gene            complement(6623..7573)
                     /gene="ftsY"
                     /locus_tag="PLN75_03330"
     CDS             complement(6623..7573)
                     /gene="ftsY"
                     /locus_tag="PLN75_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRG03183.1"
                     /translation="MAFFGLFNKEKKETLDHGLAKTKENVFSKLTRAVAGKSKVDDEV
                     LDNLEEVLVTSDVGVETTLRIIERIENRVSRDKYLNTNELNSILKEEIAALLAENNAA
                     QVNDFDAPLPSTPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKKVYLGAADTFRAAAVD
                     QLVIWGERVGVPVIKQKMGSDPASVAYDTLASAKANNADVVIIDTAGRLHNKINLMNE
                     LTKIKNVMQKLIPEAPHEILLILDGSTGQNAYEQAKQFTKATEVNALAVTKLDGTAKG
                     GVVIGISDQFKIPVKYIGLGEKMTDLQVFNKEEFVKSLFD"
     gene            complement(7757..7909)
                     /locus_tag="PLN75_03335"
     CDS             complement(7757..7909)
                     /locus_tag="PLN75_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRG03184.1"
                     /translation="MAKKAVASMQKGEGRTFSKVIKMVKSPKTGAYTFQEEMVHNEKV
                     KEYLTK"
     gene            complement(7922..8110)
                     /gene="rpmG"
                     /locus_tag="PLN75_03340"
     CDS             complement(7922..8110)
                     /gene="rpmG"
                     /locus_tag="PLN75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRG03185.1"
                     /translation="MAKKSKEARVQVILECTEHKESGMPGTSRYITTKNRKNTPGRME
                     LKKYNPILKKMTVHKEIK"
     gene            complement(8129..8368)
                     /gene="rpmB"
                     /locus_tag="PLN75_03345"
     CDS             complement(8129..8368)
                     /gene="rpmB"
                     /locus_tag="PLN75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRG03186.1"
                     /translation="MSKICQITGKKAMVGNNVSHSKRRTKRTFDVNLFNKKFYWVEQD
                     MWISLKISAAGLRTINKKGLDAAIKQAAEKGYLYV"
     gene            complement(8556..9788)
                     /locus_tag="PLN75_03350"
     CDS             complement(8556..9788)
                     /locus_tag="PLN75_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRG03187.1"
                     /translation="MIEIITIGDEILIGQIVDTNSAWMAVELNKAGFLVSQITSVHDD
                     AQQIKAALDLALSRNSIVLITGGLGPTKDDITKLTLCDYFQTKLVFIDSVLRNVENMF
                     RRRNIAINELTRNQAYVPESCTVIQNPVGTAPIMWFDHAGKVVVSMPGVPFEMKTVMT
                     DEIIPRLHLKFQSKPVVNQTVQVYGYGESALAIKIADWENALPPYLKLAYLPGFGIVK
                     LRLSGTLDDGEKLLSEIERQFALLDQIIGDSIVFRNDFPVEVNLGNALREKKLTISAA
                     ESCTGGNIARRLTSVPGSSDYFTGSVTAYKNEIKEQLLRVDAGDLKNSGAVSQPVVEQ
                     MAEGIRILMNTDIAVATSGIAGPAGGTDEKPVGTVWIAVSTPQKVESRRYQFGKSTRE
                     NIIERATIAALMMVTEVL"
     gene            complement(9877..10458)
                     /locus_tag="PLN75_03355"
     CDS             complement(9877..10458)
                     /locus_tag="PLN75_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRG03188.1"
                     /translation="MLQIENTIISPDLISERFVCDLASCKGACCIEGDNGAPLEENEI
                     EIIEQLLPVVWDDLTEVSKEVIREKGFFYIDEDNEPVTSIVNGRECVFTYTDENGICK
                     CAIEKAFREGKTDFYKPVSCHLYPVRLQHYTGFTAVNVHRWNVCDCARTLGSRLRIPV
                     YVFLKEPLIRKFGNEWYEQLEIADKELFSKNNK"
     gene            complement(10503..11135)
                     /locus_tag="PLN75_03360"
     CDS             complement(10503..11135)
                     /locus_tag="PLN75_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRG03189.1"
                     /translation="MELNYINIIDYLGTFAFAISGIRMAASKRFDWFGAYVVGLVTAI
                     GGGTTRDLLLGITPFWMIQPSYLIVTGIALLFVIVFGKWVVRLNYTIFMFDAIGLGLF
                     VVVGIDKTITHGFPAWVAVAMGMITGSVGGIIRDVLINQVPLILRKDIYAMACIIGGL
                     IYFACIRMNFSPFATQSVSLVSVILIRVLSARYHIGLPLLKYQEEDKPEN"
     gene            complement(11288..11674)
                     /gene="secG"
                     /locus_tag="PLN75_03365"
     CDS             complement(11288..11674)
                     /gene="secG"
                     /locus_tag="PLN75_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRG03190.1"
                     /translation="MYITLIILIIIAALLLTLVVLVQNSKGGGLAAGFQSSNQIMGVR
                     KTTDFLEKATWGLAAFIIVISILAVGVKSNMASENTNDSAIKEQSADINKQMQQGTAV
                     PNFGEVPQAQPAQAEKPAPEAQPAKK"
     gene            complement(11687..12343)
                     /locus_tag="PLN75_03370"
     CDS             complement(11687..12343)
                     /locus_tag="PLN75_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03191.1"
                     /translation="MFINLPEIEMTYDDFIHYIRQPETLKDCQPEELKKLADKYPYFK
                     LSKWLYLNSLHKTGSVYFGQELKKTALYAADRRNLYFYIHPDESAVPEPSVLRSDNAG
                     SYFDMIGRLDTKTENSRMSLQTLAEKLKAARETLKTTTAQTHGDIPTRPESEEQKAEP
                     NQSEVFEEKEKQAKQLIREKKYAEAVKILEELNLINPKKSIYFADQIRFLKKIIQNQQ
                     "
     gene            complement(12429..12953)
                     /locus_tag="PLN75_03375"
     CDS             complement(12429..12953)
                     /locus_tag="PLN75_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRG03192.1"
                     /translation="MIRLKKIFISIHVLVFVMILTGCSVKYSFNAGAIDYSKYSSISI
                     ADFPNNAELVNPTLSQIFAETLRDKFTRQTRLQVLKQNGNMHLEGEIVGYQLAAMAIS
                     ADSYASETKLTLTINVRFVDSKNPENSFNDSRYIASQIFDSNRLLSDVEPELIDILVK
                     EIVDKIYNDTVAKW"
     gene            complement(12960..14201)
                     /locus_tag="PLN75_03380"
     CDS             complement(12960..14201)
                     /locus_tag="PLN75_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRG03193.1"
                     /translation="MNHSELHTVKQRFGIIGNSEILNRAIDIAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHNYSHRKHGAYIAVNCGAIPEGTIDSELFGHEKGSFTGATADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLEAGEFIKVGSSKTQKTDVRVVAATNINIM
                     QAIRDGRFREDLFYRLNTVPIFIPPLRERREDIPLLFRKFAADFAEKYSMPPVRLSED
                     ARQILMNYYWNGNVRQLKNITEQISVIEQQREINASTLRMYLPNENTEKLPAIIGGSK
                     SENKTFNNEREILYQVLFDMKKDISDLKKVVNELSSAQIQTDVSYSPTILQGDNYVNQ
                     MNHPTEKSVKFNDVDDVEPIQEIEDYKEEDEKVSLEDMERELIRKSLEKHKGKRKIVA
                     RELKISERTLYRKIKDYEIEI"
     gene            14585..15691
                     /gene="ald"
                     /locus_tag="PLN75_03385"
     CDS             14585..15691
                     /gene="ald"
                     /locus_tag="PLN75_03385"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRG03194.1"
                     /translation="MKIGIPKEIKNNENRVSLTPSGAHTLTVNGHEVFVQTNAGMNSG
                     FTDEQYLKAGAQILPDAKSVFDIAEMIMKVKEPIESEYELIKPNQLVFTYFHFASDET
                     LTRAMIKSKAVCLAYETVELNNTLPLLLPMSEVAGRMATQQGAKYLEKPQGGKGILLG
                     GVPGVKPANVLVIGGGVVGQQAALMAAGMNANVTIFDVSLPRMRYLSEIMPKNVDTLM
                     SSKYNIEALLPAADLIIGAVLIPGAKAPHVITREMLNLMQPGSVLVDVAIDQGGCFET
                     SHPTSHADPIYKVNDVVHYCVANIPGAVPFTSTLALTNATLPYAVMLANMGWEKACAK
                     YPDLRLGLNIVDGKVVYKAVADTFGLPFEELILQ"
BASE COUNT         4018 a         3471 c         3319 g         4980 t
ORIGIN      
        1 ttagaagtta gaaattagaa attagaagtt agaagcctgt ttgcctctgg cagattagaa
       61 attagaagtt agaaattaga aattagaaat tgtaaattgt aaatcaaaat aaggtgctga
      121 caaaaagaat cattccgtgc ctggacgtaa aagacgggaa aacggtgaaa ggaattaact
      181 ttgtggacat cagggatgtg ggtgatccgg tggaactggg gaaacgctat gccgaaatgg
      241 gtgccgacga acttgttttt ctggacataa ccgctacaca cgaaaagcgg aaaacgctct
      301 cggaactggt tacacatata gcacgtgaaa tcaacattcc atttacagtg ggtggaggaa
      361 tttcatcggt ggaggatgta tcgctgctgc tgagctgcgg ggctgataaa atatcggtga
      421 atacggcagc agttcgcaat cctcagttga tagccgatct ggcagataat ttcgggagtc
      481 agtgtgtggt gctggctatc gataccaagt gcatcgacgg tgagtggtac gtttttctga
      541 acggcggccg catcccgact gaaataaaaa caatggactg ggccagacag gctgtggcac
      601 tcggagtggg cgaaattctg cttacttcca tgaatcacga cggtacgaaa gacggatttg
      661 ctactgatat tacccgtgct gtatctgaag ctgtgaatgt tccggtgata gccagcggcg
      721 gtgctggaac catgcagcac tttgtggatg tattcaatca gggcaaagcc gatgcggcac
      781 tggctgccag catctttcat tacgaagaaa ttggcattgc cgaactcaaa cattatctga
      841 aagaaaaagg cattgccgtc agaatttgac agcaatgcct ttcgtttaca gttatcgact
      901 ttcctatttg aggtattccg actgtaattt caacgcatag ttcagacact ccctgcttgc
      961 cggatccgcc agtcgggcat ccagtttcgg aattttaata ttaatgccca gggggacgtt
     1021 gttcggatct ttgattgcct ctctgttggc ttcataaata tacacccaga agtgtggatg
     1081 tccgtaatac ttacgggcaa atttggtaag ttgacttccg gcgaccattt tctctgtggt
     1141 aatcagttcg gtgtaatttc tgggagcatc aaatatattt ccggcggcag gtgcttctga
     1201 aataactgtt tgcaccgatt catcggtagc ttttacagca acggtgtcgg ttgttgcaat
     1261 agaatcgccg gccggtgtca tcgaatccag cacttctttt gtgttcaaag cccgaaccgt
     1321 agaatcggcc aggtattcca tgtacatggc atccttttgt ttatcgagga tatttttgac
     1381 aaaatagaat gcaccaaaaa acaccaggcc gagtgctgct attcccaaca aagtgtaaag
     1441 ccagacaggt cgttttttct tttcctcaac atgataatgt ctccttcttt tgtgcgatga
     1501 agatgtactg acaattttct ttttatcagt caccgtacgg gcatttttaa ctgcaactaa
     1561 aggttctaca gacttttctt tggctgctgt attatcagcc tcaatggttt ctgcagcttc
     1621 ttcatcagtc tgagtcacga cttcaggcaa ttcaaccacg gtttcatccg tatcttcgtc
     1681 gttgtcatct ccatcttctg cctctgtatc atcctctggt atcagttgtt catcttcatc
     1741 tgtttcatca tctgcttcat cttcgtctga ttcttcgtct gtttcatcat ctgcttcatt
     1801 ttcgtctgtt tcatcatcgt cgtcaatttc ctcaaacttt tcttgtttct cgtcatcatt
     1861 gttgtcatct tcggcttccc gatcgtcgat ctcgtgtttt tcggcggcaa cggtattttc
     1921 cggtatttct tcgggtagta cttcagaaat attactttca gtttctgatt cagcttctgc
     1981 cggtaactgg tcagattctt cattaattaa agcaactgca gctgcgccgg ccggtaaacc
     2041 aaatctggtt gaaatttccc tgacaatatc aaaaccatcg aaattaaatt ctgtttcctt
     2101 atttccggta tgctcaggcg tatgcaaggt ttccggagcc gaaataattt ccgctacttc
     2161 agatactgat tccggtttat ccattggagt attttcaagc gtgatttcct cctctgtttt
     2221 ttcgtccgga tccggtaact gcgagctttc attctccgta acagtatcat tcgacctgac
     2281 tttcggcgtc gaaaaagaat tgatttccga cagcagctct tttatttctt ccgcctgttc
     2341 accgaataca cgcaatggtt ccaggggttc tgaaaagttt tccggttcat cttcctccgc
     2401 gtcatcctgc atttgttcgg gcaaattatc ggacagcgga ttcagttcca cgggttcgag
     2461 gtgcgaaaat ggttcgttga tcagctctct caagtcattt tcgggaatga agacagcttt
     2521 gtaatatcca tcgataacaa tctcctcgcc tgtattcaca ttcacgcttt tgcgggcttc
     2581 gttccattgc gatttgaagg ttcccaatcc ttttattttt actgtttcac cgtctttcaa
     2641 agcgtcttcg atgacagaaa aaaacacctt aacaaattct tcggctgatt ttttggttat
     2701 catccggtct tttaccatta gttcaacaat ttcgcttgct gataattttg cattactcat
     2761 actatcagaa ttttacgtta agtttatctt tcagaaccga actttgtttg aaacttacca
     2821 ccaatttcgg cggaatcatt gtgcgcacct tcgtaagggg gtgtaccgac aatcgttctt
     2881 cttttttacg tatttcaaac gatccaaaac cctgaaaacc aattgaatta tcctctttca
     2941 actgatttct gaaagtttcc gtaaaagctt ccagcaggta tttcacctca tttttgggta
     3001 aattcatttt cgaagctaca tttgaaatta attccttgtt gttcatatat ttaatttatt
     3061 agaatacaaa aatttaattg aaattttcgt gagtaatgtt tattcgccga acagttcgaa
     3121 atcggtagcg tcaacaatgg ttgcctgata aaactcaccc actctcacac cctttgtatc
     3181 agcagggatc attacttcgg gatcaacttc cggtgagtca aattcggtac gtccgataaa
     3241 ataatcaccc tctttccgat ctataatcac tttgaggatg ctgccggttt tcgcggcatt
     3301 gatttccgca gctattcgtt gctgtatgtc catcagttcg cccagccgtt gctgttttgt
     3361 ttcgtcggga atgttatccg tgtaattttt gtgggcataa gttccctctt cgtgtgagta
     3421 ggcaaaagct cctagtcgct cgaaacgggc ccaccgtaca aattccttca gttcttcaaa
     3481 gtcctgttcg gtttcctccg gatggcctac caacatggta gttctgaggt gaattccggg
     3541 cacttcgctg cggattttat cgattagttc ataggtttga gccgatgtaa aattccgtcg
     3601 catgacaccg agcatgtgat cgctgctgtg ttgtaaagca atgtcgaggt atttgcatac
     3661 attttcacgt tcgcgcatca cccgcagcac gtcgtacggg aaatgggccg gataggcata
     3721 gtggagccga atccagccaa caccctgtat atccgaaagc cgctcaacca gttcagcgag
     3781 tttggatttt ttatagcggt cgatgccata ataggtcaga tcctgcgcta tgagctgaaa
     3841 ttctttcacc cctttctcaa ccaacaattt tacctccttt tcgatgtctt cgatcggccg
     3901 cgaccgatga cgccccgtga taatgggtat tgcacagtat gaacaggtac ggttgcaacc
     3961 ttcggaaatt ttgatgtatg cataatgcag cggggtggtg agtgtccgct ccagcttcag
     4021 atcggcgcgg tattcctgcc cgatttcctt caggatatcc acgtagttga actttccgta
     4081 aaacttatcc acttcgggaa tttcggtttc cagatcgtgc agataccgct cggataagca
     4141 acccatcacg aacaacttat tttttaattc cctttttctt tccgccaact gcagaatcat
     4201 gttgatgctt tcctctttgg catcgcctat aaatccgcag gtattcacaa tgacaatttc
     4261 cccgtcggga ttttcggcat cgtgccgtac attatagccg agtacgtcaa actggcgcat
     4321 cagctgctcg ctgtccacca gattcttgga acatccgagt gtgataatat ctacggtatt
     4381 ttttttcata agctacgtat tcctgatttt ttttattcac tgcacgacaa aatattctcc
     4441 gcagaaaatg cggttaatgt gctgtttatt ctccgcaaaa tttatttttt tacccaacgg
     4501 catcgttgaa ccggtaaaag gatgaaacgg ataattacca tttgctataa taaaaattct
     4561 tgcaggtttg tataaaaaat atgaaactac aatttggtaa ttttcattgc aaatcctcct
     4621 cccggagcca ttttcagcgt cattttcctg tcagcggaca catcaactgt ttcctttcgg
     4681 tagtccattg ccgtacggtg tgcattcacg ccgtcgcgga aaatctcggc tttgaaattt
     4741 ccatcgccca ggaatgataa atcgacatcc agtgtacggc tgtcccagtt tgtcattgag
     4801 cccaggtacc actcgttccc ggttcggcgg gcaatggtta cgtattttga tatctcgccg
     4861 ttgagcgcta ccgtttcatc ccaaacagta gggatttcgg caataaactg cgtgcattcg
     4921 ggctccgcca ggtaattgga aggattatcg caaagcatat taaacggtgc ttcgaaaatg
     4981 acatactgcg ctaactggcg gcagcgtgta ccctgactca tgggttcgga atagacagga
     5041 cgataattgt ttcgggtagc atttcgcatg gagccctgtg tgtaatccat cggaccggcc
     5101 accatccgga tgaaaggaat ggtgacatcg taggttacct gatcgacact tgccggcgac
     5161 catttcatct gctcgagtcc gtgaacacct tcgaaattaa tcacgttcgg ataggttcgc
     5221 tgaagtccgg tgggtttata ggctccgtga aaatcaagca tcagattgta tttggctgct
     5281 atctgagcag cccggtggta aaaatcaacc atctgctggt cgtcccggtc cataaaatct
     5341 accttgaaac ctttgattcc catttcagca taatgtttgc aaattccctc tatatcgcgg
     5401 ttgaaggccc agtaaccggc ccatagtatc aggtccacct ttttggattt gccatattca
     5461 accaactccg tgagattgat ttcgggaatt acctgaaaca agtcggcttt tttgtttacc
     5521 gcccagcctt cgtcgagaat cacatattct atgccttgtt cagctgcaaa atcaatgtag
     5581 tatttatatg tttcgttgtt gattcctgct tcaaaatcta cattccggat attccagtcg
     5641 ttccaccagt cccaggctac ttttccgggt tttatccagg aaatatcggc taactgattg
     5701 ggagtggcga gtttataaac catgtcgttg tcggttaatt cgtaatcatt ttctgcgatg
     5761 atcactgtcc gccacggaaa agtggttttt ccttcacacc gggcaataaa gttttcggcc
     5821 gattgcactt ctccctgaag catattgtgc ccaccctgtc ttatttccct cggataagga
     5881 gcaaacatgg cagtgagaga actggttcca tcgggattgt agagatacat gccgggataa
     5941 ttgagcagat cggcttcggt gatgcacaca tttttaccat ttttaccctc gactaacaaa
     6001 ggcaggaaag ccagcaaacg tggatttgcc tctttcagtg gaatatgatg ataggtattt
     6061 tcgaaagaat tgtaaaattg ctgactgaaa gtggtgttgt cggatttgtt tacatacgcc
     6121 agaaatgctt tgggatcgtc cgggaaattg aatttcgctt cttcattctc aacggtgtaa
     6181 ggttttttga agttggtttc gaagcgataa gccactccat cgtcataagc tctgaaaatt
     6241 aggctgtaat ctcctgagaa cagaatattt agctcattat aattattttc gactttcgat
     6301 tttttgtaaa ttttggcatc aatgatttca ttcaccgttt tcttaacagc ttttttcacc
     6361 tttgcatcgg gtccgggtat tttaccttcg gataatttca tcgaaacagg tgatttttcg
     6421 agcatcagat cgccgttgtg cgtgatggcg aattcggttg ttttaccaac tgaaatttcg
     6481 gtcttcaact ttccgtcggg cgacaggagc acgaattgtt tttgcgaaaa agccaaagtt
     6541 gtggcgcata tgaggatgaa tgtcaataga tttctcatga tattttttta aaaaatttga
     6601 taatcaggtt taacggaggg aattaatcaa acagggattt tacaaactct tctttgttga
     6661 acacctgcaa atcggtcatt ttttcgccca gaccgatgta tttcaccgga attttgaatt
     6721 gatcggaaat accgatgacg acgccgccct tggcagtacc gtcgagtttg gttactgcca
     6781 gcgcatttac ttcggtggct ttggtaaatt gtttggcctg ttcgtaggcg ttctgtccgg
     6841 tcgatccgtc gagaataagc agaatttcgt gaggtgcttc ggggatgagt ttttgcatta
     6901 cgtttttaat tttcgtaagt tcattcatca ggtttatctt gttgtgaagc ctgcctgccg
     6961 tatcaataat gaccacatct gcattgttgg ctttggccga agcgagtgta tcgtatgcca
     7021 cagaagccgg gtcggaaccc attttttgtt ttatcaccgg cactcccacc ctttcgcccc
     7081 agataacgag ctgatccact gcggcagcac ggaatgtatc ggcggctccg aggtagactt
     7141 ttttaccggc ctgtttgaac tgataggcca gttttccgat ggtggtggtt ttacctactc
     7201 cgttgacgcc taccaccatg attacataag gtgttgaagg cagcggagcg tcaaaatcgt
     7261 tgacctgtgc tgcattattt tcggccagca aagccgcgat ttcttccttc agaatactgt
     7321 ttaattcatt cgtgttcagg tatttgtctc tgctcacgcg gttttcgatg cgttcgatga
     7381 tccgcagtgt ggtttccact cccacgtcgg aagtgacgag cacttcttcg aggttatcga
     7441 gcacttcgtc gtccactttg gatttgcctg ctacagcacg tgtgagtttt gagaatacgt
     7501 tctctttagt tttggcaaga ccgtggtcaa gtgtttcctt tttttcttta ttgaatagtc
     7561 cgaaaaaagc cataattagt tttgagttta ttgtctgtag ttttaagcct gagagatatt
     7621 ggcaaatctc atacttcagg tgatagtttc tgatttttga ccaatcatac aaaaataatt
     7681 cttttttagg tataaaacaa aaaaatcctt tcattgtact aatgaaagga tttttttaat
     7741 gcttgtatcg aaaagattat tttgtcaaat actcttttac tttttcgttg tgcaccattt
     7801 cttcctggaa tgtatatgct ccggttttag gcgatttaac catcttaatc accttggaaa
     7861 aggtacgacc ttcacctttt tgcatacttg caaccgcctt ctttgccata gtttacttat
     7921 tttatttaat ttctttatga actgtcattt tcttgagaat aggattgtat tttttcaatt
     7981 ccattctgcc cggtgtgttt tttctgtttt tcgtagtaat gtaacgagaa gtacccggca
     8041 taccactttc cttatgttct gtgcattcca gaataacctg aactcttgct tcttttgatt
     8101 ttttagccat tgtattttct ccctgtgatt aaacgtataa ataccctttt tcggctgcct
     8161 gtttgatagc agcgtccaat ccttttttgt tgatagtgcg tagtccggca gctgaaattt
     8221 tcaggcttat ccacatatcc tgttctaccc agtagaactt tttgttgaac aagttcacat
     8281 caaatgtacg tttagtacga cgtttggagt gtgaaacgtt gttaccaacc attgcttttt
     8341 ttccggtgat ttgacaaatt tttgacatct ctgtatctct taaatttttc ttaatatttt
     8401 ttctcaaaaa cagtgtgcaa aggtagaaat ttattttctt tcctacaaac tttttgagtt
     8461 catatttttg tttttatttg atttaacaat aattttgagg tttctaccct cattttcaaa
     8521 gcatccgtgt atttttctcc cggatgaaat tcctgtcaga gcacttctgt taccatcatc
     8581 agtgcggcga ttgtggctcg ttcgatgatg ttttcgcggg tgcttttacc gaactgatac
     8641 ctgcgacttt ccactttttg gggtgtgcta acggctatcc acacagtacc aaccggtttt
     8701 tcatcggtgc ctcctgcggg cccggcaata cccgaagttg ccactgcaat atcggtattc
     8761 atgagtatac gaatcccttc ggccatctgc tctacaacgg gttggcttac ggctccggaa
     8821 tttttcagat ctccggcatc gacacggagc agttgttcct ttatttcatt cttgtatgcc
     8881 gtgacagaac cggtgaaata atcggagctt ccgggcaccg aagtaagcct gcgggctatg
     8941 ttgccaccgg tgcaactttc agctgccgaa atagtcagtt ttttttcacg taatgcattt
     9001 cccagattca cctcaaccgg aaaatcgttt ctgaaaacaa tcgaatcgcc gattatctgg
     9061 tcgagcaatg caaactgacg ttcaatttcg gacagaagct tttcgccatc gtccagcgtg
     9121 ccggagagcc tcagttttac aataccgaaa cccggcagat aagccagttt cagatacggg
     9181 ggaagtgcat tttcccagtc ggctattttg atggcaaggg ctgactcacc gtatccatat
     9241 acctgaactg tttgattgac aaccggtttt gattgaaatt tcaggtgcag acgggggata
     9301 atttcgtcgg tcatgaccgt tttcatttca aatggcacgc cgggcatcga aactaccact
     9361 tttcctgcgt ggtcgaacca catgatggga gctgtaccca ccggattctg aattaccgtg
     9421 caactttcgg gcacgtaagc ctgatttcgg gtaagctcgt tgatggcgat gtttctgcga
     9481 cgaaacatat tttccacatt ccggagaacc gaatcaataa atacaagttt tgtctgaaaa
     9541 taatcgcaca gggttagttt tgtaatgtcg tctttggtgg gtcccagtcc gccggtgatg
     9601 agcactatgc tgttgcgtga cagagccaga tccaaagcag ctttgatttg ctgcgcatcg
     9661 tcgtgaacag aagttatctg tgaaaccaga aatccggctt tatttaattc tacggccatc
     9721 caggccgaat tggtgtccac tatctgccca atcagaattt cgtcgccaat ggtaattatt
     9781 tctatcaatt cagtagttgt ttttttttgg aagtttgttc atataattat tcaggtattc
     9841 tttcaacctg tgtcgttttc acggggtgaa tcagcatcat ttgttgtttt tcgaaaataa
     9901 ctctttatcg gctatttcga gctgttcgta ccattcgttt ccaaatttcc ggatcaatgg
     9961 ctctttaaga aacacataga cgggaatccg aagtcgggaa cccagtgtac gggcacagtc
    10021 acacacgttc catctatgaa cgttaacagc tgtaaaaccg gtgtaatgct gcagccggac
    10081 cggatataaa tgacaggaaa cgggcttgta gaagtctgtt ttgccttcac gaaaggcttt
    10141 ttcgatggca catttacata ttccattttc gtcggtgtag gtaaatacgc attcccggcc
    10201 attcacaatg gacgtaaccg gttcgttatc ttcgtcgatg tagaaaaatc ccttttcgcg
    10261 gataacttct ttggaaactt cagtcaggtc atcccaaaca acgggtaaaa gctgttcgat
    10321 aatttctatt tcgttttctt ccagcggtgc tccgttatca ccttcgatac aacaggcgcc
    10381 tttgcagctc gccagatcgc acacaaaacg ttcgcttatt aaatcaggac tgatgatggt
    10441 attttcaatt tgaagcattc tattgattga caattaattg atttacaatt ttcaaaaaaa
    10501 aatcagtttt caggtttgtc ttcttcctga tatttcagaa gaggcagtcc gatatgatat
    10561 ctggccgaca aaacacgtat gagaataacc gaaacgaggc taactgactg agtggcaaaa
    10621 ggactgaaat tcatcctaat gcaggcaaag taaatgaggc cgccaatgat acacgccatg
    10681 gcgtaaatat cttttcggag aataagcggc acctgattga tcagcacatc tctgataatt
    10741 ccgcctacag accctgtaat cattcccatt gctactgcca cccatgcagg aaagccgtgt
    10801 gttattgttt tgtcgattcc cacaactacg aaaagcccca atccgatagc gtcaaacatg
    10861 aatatggtgt aattgagcct tacaacccat ttaccgaaaa cgataacaaa taaaagcgcg
    10921 atacctgtca cgatgagata agagggctga atcatccaga aaggggtaat tccgagcagc
    10981 aaatcgcgtg tggtaccgcc gccaattgcc gtaaccaaac caactacata agctccaaac
    11041 cagtcgaatc gtttggaggc agccatccga attccactga ttgcaaatgc gaatgtgccc
    11101 agataatcaa taatattgat gtaattcaat tccattgtca gatattcagg tgttcggatg
    11161 ttcgggtgtt cggtttattt aatttcaaca agctgaagtt tttctctttc agcacttttc
    11221 aaaacaaaaa acgtatcaaa aatcaaattt gatacgcttt ttttttcagt cattattttt
    11281 tgacttttta ttttttagcc ggttgtgctt cgggagcagg tttttcggcc tgcgcgggct
    11341 gagcctgagg cacttcaccg aaattaggaa ctgctgtacc ttgttgcatc tgcttgttaa
    11401 tatcggctga ttgctcttta atggccgaat cgttcgtatt ttcggaagcc atattcgatt
    11461 ttacacctac tgccagtatt gaaataacga taataaaagc tgccagtccc caggttgctt
    11521 tttcaagaaa atcggtggtt ttacgcactc ccattatctg attggatgat tgaaagcctg
    11581 ctgccaaacc gccgcctttt gaattctgaa cgagtactac aagggtaagc aataatgcgg
    11641 caataataat gagaataatt aatgtaatat acatatctgt gtctgtttat tgttgatttt
    11701 gtattatttt tttcaaaaat cgaatttggt ctgcaaagta aatacttttt tttggattaa
    11761 tcaaatttaa ttcctctaaa attttaacag cttctgcgta ttttttttcg cggataagtt
    11821 gtttggcttg tttttctttt tcttcaaata cttcactttg atttggctct gctttttgtt
    11881 cttcactttc gggccgggtt ggtatgtcac cgtgagtctg tgcagtagtg gtttttagcg
    11941 tttcgcgggc agctttcagc ttttcggcga gcgtctgcag tgacatgcgg ctattttctg
    12001 ttttcgtgtc gagccggcct atcatatcaa aataacttcc ggcattatca cttctcagca
    12061 ccgaaggttc ggggactgct gattcatcgg gatgaatgta aaagtacaga ttacgtctgt
    12121 cggcagcata aagtgctgtt tttttcagtt cctgcccgaa ataaacactt cctgtcttgt
    12181 ggagtgagtt caggtaaagc catttggata gtttaaaata cggatattta tcggctagtt
    12241 ttttcagttc ttcgggctga caatctttga gcgtttcggg ctgccggatg taatgtatga
    12301 aatcatcgta agtcatttct atttcaggta gatttatgaa caaagttcaa agatacaggg
    12361 taatggagag ggtttcaggc ttgtaatcaa gcaaaaaaac accttatttc aaccaacaat
    12421 ccaaaacatt accattttgc caccgtgtcg ttgtaaattt tatctacgat ttctttcacc
    12481 agaatatcaa tgagttcggg ttccacatcg ctcagcaggc ggttactgtc aaatatctga
    12541 gatgctatat agcgggaatc attgaaactg ttttcgggat ttttggaatc gacaaaacgg
    12601 acattaatcg tgagtgtaag tttcgtttcc gaagcatagg aatcggcact gatggccatg
    12661 gctgcaagct gataaccaac tatttcgcct tccaggtgca tattcccgtt ttgtttcaga
    12721 acctgaagac gtgtttgccg ggtgaattta tccctcagtg tttcagcaaa tatttgtgaa
    12781 agtgtcggat tcaccagctc ggcattgttg ggaaaatcgg caatggaaat gcttgagtat
    12841 tttgagtagt cgatggctcc ggcattgaaa ctgtatttta ccgaacatcc ggtcagtatc
    12901 atcacaaata caagtacatg aatactgata aatatctttt tcaatctaat cattcagatt
    12961 taaatttcaa tttcgtaatc tttaattttt ctatacagtg tccgctctga tattttcaat
    13021 tcgcgggcta caattttacg tttgcctttg tgtttctcga gtgattttct gatcagttcg
    13081 cgttccatat cttccagtga taccttttcg tcttcttcct tataatcctc tatttcctga
    13141 ataggctcca cgtcgtccac atcgttgaat ttcactgatt tttcggtcgg gtgattcatc
    13201 tgattcacat aattatctcc ctgcagaata gttggagagt agctcacatc tgtttgtatc
    13261 tgtgctgagc tgagttcatt cactaccttt ttcaggtccg aaatatcttt tttcatatca
    13321 aaaagaacct gataaagtat ctcgcgttcg ttgttgaatg ttttgttctc agattttgag
    13381 ccgccaatga ttgccggtaa tttttcggta ttttcattgg gcagatacat gcgcagggtt
    13441 gaagcgttta tctccctttg ctgttcgatg accgaaatct gttcagttat gtttttcaac
    13501 tgacgtacgt tgccgttcca gtagtagttc atcaatattt gtcgggcatc ttcactcaac
    13561 cgcacgggag gcatgctgta tttttcggca aaatcggctg caaatttcct gaacagcagc
    13621 ggaatatcct ctctcctttc cctcagcgga ggaatgaaaa taggcacggt gttgagccgg
    13681 tagaaaagat cttcgcgaaa tctgccgtcg cggatagcct gcatgatgtt tatattggta
    13741 gcagccacca ctctcacgtc ggttttctga gttttggacg aacccacctt aataaattca
    13801 cccgcttcaa gcacacgcaa cagcctcacc tgtgtggaca gcggcagttc acccacttca
    13861 tccagaaaaa ttgttccacc gtcggcaact tcgaagtaac ctttacgatc ggctgtggca
    13921 ccggtgaacg agcctttttc gtgaccgaaa agttcggagt caatggttcc ttcagggata
    13981 gctccacagt tcacggctat ataggctccg tgtttacggt gactgtaatt gtgaataatc
    14041 tgagggaaat tctcctttcc tactccgctt tcacccgtta ttaacacaga aagatctgtt
    14101 ggagccacct gaaccgcaat atcgatggcc cgattcagaa tttcggagtt acctataatt
    14161 ccaaatcttt gtttcacagt atgtaattcc gaatgattca tttatatatt tcaaatgtgt
    14221 aaaaaaacta acaaaattgc cgaattaata actgacaaaa ttacaaaagt ttttaataag
    14281 taagaattat tatttggtta aaaaaggttt taatcatttt ttcggcacat tgccgaagag
    14341 ctgaatcggg tatatgacaa ctctgtattt tttacacaaa cacatcagat aagcagaatg
    14401 ctgaaaaaat atatttcctg aaacagaaaa tgcagatact gaggcataaa aaaccgttaa
    14461 tggccaaatt tatatatctt tatgtaattt tttttctgat ttatcgataa aaaacatgaa
    14521 aaacatgtac attttgtcaa aaatgttatt acttttacga ccaaaacttt tataacaaat
    14581 aaccatgaaa ataggtattc caaaagaaat caaaaacaat gaaaacagag tttcactgac
    14641 tccctcgggg gcacatactc tcaccgtaaa cggacatgaa gtcttcgtac aaaccaacgc
    14701 cggcatgaat agcggattca ccgatgagca atacctgaaa gccggtgctc agattttacc
    14761 ggacgcaaaa agtgtatttg atattgcaga aatgattatg aaggtaaaag agcctattga
    14821 aagcgaatac gaactcatta agcccaatca gctggtattc acatactttc acttcgcttc
    14881 tgatgaaacg cttacccgcg ccatgattaa gagtaaggca gtttgtctgg cttacgaaac
    14941 ggttgaactc aacaatacgc ttccgctttt gctacccatg tcggaagtgg ccggaagaat
    15001 ggctactcag cagggtgcca aatacctcga aaagccgcag ggcggaaaag gaatactgct
    15061 gggcggagtt cccggcgtaa aaccggccaa cgtactggtt atcggtggcg gtgtggtagg
    15121 ccagcaggca gcactgatgg ctgccggtat gaacgccaac gtcacgatat tcgatgtttc
    15181 gctgccccgc atgcgctacc tgagcgaaat aatgcctaaa aatgtggata cgcttatgtc
    15241 aagcaaatac aacatcgaag cgctgctgcc tgcagccgac ctgattatcg gagccgtatt
    15301 gataccgggc gccaaagctc cccacgtaat aaccagagaa atgctcaacc tgatgcagcc
    15361 gggctcggta ctcgttgatg ttgccatcga tcagggcgga tgctttgaaa cttcgcaccc
    15421 cacatcacac gcggacccga tttacaaagt aaacgatgtg gtgcattatt gtgtggccaa
    15481 tatccccgga gcggttccgt tcacttctac ccttgcactc accaacgcta cgctgcctta
    15541 cgcggtgatg cttgccaata tgggttggga aaaagcctgt gcaaaatacc ccgatctgag
    15601 gcttggatta aatatagttg acggaaaagt ggtttacaaa gctgtagctg acacattcgg
    15661 tttgcctttc gaagagttga tactgcaata aatagttgta tcaataccgg aatactgccg
    15721 tgcaaaaacc acattctgca cggcagtttg tattttaaaa gaaacacaga aaacgcgtgc
    15781 catataaa
//
LOCUS       DAORKP010000035        15738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_266_length_15738_cov_8.096091, whole genome shotgun sequence.
ACCESSION   DAORKP010000035 DAORKP010000000
VERSION     DAORKP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_15738_cov_8.096091"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1030
                     /gene="gap"
                     /locus_tag="PLN75_03390"
     CDS             32..1030
                     /gene="gap"
                     /locus_tag="PLN75_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRG03195.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQQRNDIEIVGINDLISVDYMSYML
                     RYDTVHGQFKGTVEVKDGHLVVNGKTIRVTAEKDPSNLKWNEVEAEYVVESTGLFLTK
                     ETAEAHIKAGAKRVVLSAPSKDDTPMFVCGVNTDTYKGQAIVSNASCTTNCLAPIAKV
                     INDKFGMVEGLMTTVHSTTATQKTVDGPSAKDWRGGRAASGNIIPSSTGAAKAVGVVI
                     PELNKKLTGMSFRVPTLDVSVVDLTCTLAKPATYAEICAAMKEASETYLKGILGYTEE
                     EVVSSDFIGDARTSIFDAKAGIALTDKFVKLVSWYDNEWGYSNKVLDLIAVMDKVK"
     gene            1199..1693
                     /locus_tag="PLN75_03395"
     CDS             1199..1693
                     /locus_tag="PLN75_03395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRG03196.1"
                     /translation="MNSFAAIDFETANQHATSVCSVGIVVVREGEITEKFYSLIKPEP
                     EFYSYWNVQTHGITLEDTQFSPVFPHVWEQIEPMIEGLPLVAHNKGFDERCLKEVFRM
                     YQLDYPDYEFHCSLQMARKHLKGLLPNYRLHTVSEFCGFELKNHHHALADAEACAWIA
                     RYFQ"
     gene            complement(2062..4299)
                     /locus_tag="PLN75_03400"
     CDS             complement(2062..4299)
                     /locus_tag="PLN75_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03197.1"
                     /translation="MKKLLVSILLLLSIQCSFGQWNTDRIMSIGRNALYFEDYILSIQ
                     YFNQVIKVKPYLPEPYMYRAMAKINLGDYLGSEQDCNEAIRLSPFIPYAYYTRGFARK
                     NLEKYSEAIHDFTKALEFDPDNSDYLANRIETKEKNRDYKGAIEDLKYFKKINPKAKG
                     IEYEIGRIMLSDNDTTGAEEMVRKNIEIDSTMELSYKLLAHIKEKKNDLQGAILNLKE
                     LKRINPKSENADVEMGRLYLALNDTTSALNAFDNAIENEPSKPVAYGIRAFIRMVRND
                     EKGALEDYSKAIEKGSEFVGDYINRGILNVKKYNFNQALNDYNFAILLDRSNSLAYYN
                     RALLRNNLGDKNNALTDLERVLEMDPQNYEALLQKAHLELELGMLDKTITDFESILTK
                     YKYYAPAYYALAEAYNKKGDKRTSNYYKSLVFEIVNNKDYYKRKQQLEPKNQIAKVTT
                     SAALVTQAKSLADKLSAQSEDKFKKTTYNDNLRGKVQNNYAELTYIKNADLTFEDVDS
                     VLSGTNRFHDLILNFNRKNKYNGKLKIAVNSKIMSAGNVQSHFEQIEKLSDRIRQKNS
                     DADLYFARAIEFESVQDYSSALEDLNKAIALDEQLTLAYFMRGNVRIKQMEFETNTSD
                     ADKQETGAEAFSMTEERNKINKIRIIEDYDKAIALSPDFTFAYYNKGNFLASQKDYRG
                     SVTNFSKAIEIDPNFSEAYFGRGLSYVFLNEEEPAKLDLSKAGELGIYKAYNLLNRLL
                     LKADE"
     gene            complement(4328..6607)
                     /locus_tag="PLN75_03405"
     CDS             complement(4328..6607)
                     /locus_tag="PLN75_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRG03198.1"
                     /translation="MALTEERRIGFKKVFWKIFGIGLAVVVLLFFLISIGWIGYLPEI
                     GELQNPINKSATEIYSSDAQLLGRYYKGRDNRVPINYNEIDKDIINALIATEDIRFYK
                     HSGIDLRGLIRAVFGVLTRSNSGGGSTITQQLAKQLYSPGAGNIIERAFQKPIEWVIA
                     VKLERMYSKEEIIAMYLNQFDFLNNAVGIKSASHVYFNSPADKLNIQEAATLVGMCKN
                     PSYFNPLRYNERSSSRRNVVLDQMRKAGFLTENESDSIKKLPLILDYKRVDHKEGLAP
                     YFREYLRKVLTAKKPVKSDYADWQKETYENDKWSWENDPLYGFFEKNKKSDGSKYDIY
                     TDGLKIYTTIDSRMQRYAEEAVDQHMQSIQKQFFREKKGRKYAPFSRDLTTAEVSSIM
                     QSAVNQSERYRKLKKEGAGKEEIDKSFNTKTEMRVFTYNGWVDTVMTPMDSIRYHKHF
                     LRCGFMSMDPADGHVKAYVGGPDFTAFQYDMVTLGRRQIGSTVKPYLYTLCMEEGMSP
                     CDMVLNEPITIMTSSGPWSPRNASSRRIGEMVTLRWGLKESNNWISARLMSQFTPEAL
                     VKLMHSFGIKGKIDPVVSLALGPVEVSVAEMVDAYTAFPSRGIRVDPLYVSHIEDANG
                     NIIGSFTPRMEEIFSEETAFKMIYMMRSVIDDGTGSRVRRVYGLTMPMGGKTGTTNNN
                     SDGWFMGYTPKLVSGVWVGGEERSIRFDSMAQGQGAAMALPIWANYMKKVLADKQLGY
                     KVTDDFKVSSEWSSEAGCE"
     gene            complement(6719..7843)
                     /gene="hemW"
                     /locus_tag="PLN75_03410"
     CDS             complement(6719..7843)
                     /gene="hemW"
                     /locus_tag="PLN75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRG03199.1"
                     /translation="MAGIYFHIPFCKTRCTYCDFYTQTDFSLQQDVVNAMLREIEIRK
                     TYLSEEVETIYFGGGTPSTLSAADIKNLLNAVSSFFSVNEDAEITIEANPDDLSAGYL
                     KELIKTGVNRLSIGIQSFDDLQLKAINRRHTARAALDSINVAHQTGFENISIDLIFGL
                     PGQSLESWKMQVDMAVKLRVQHISAYGLTYEQGTPLWHRMKSGKIIPTDDDTMIEMYN
                     YLVETCSKSNFEHYEISNFAKPGHRSRHNSAYWKQRPYLGIGPAAHSYDGNSRQWNVS
                     SNKKYCSFISENQPFFEKEILTLPDKYNDFVMVSMRTIEGIDLESLKNNFGEKLHDFC
                     LQSAEKYIQDSKIVRKFNFLRLTSEGIHISDKIIADMMYV"
     gene            7977..9398
                     /locus_tag="PLN75_03415"
     CDS             7977..9398
                     /locus_tag="PLN75_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG03200.1"
                     /translation="MTTRREFIRSAALASAGLAFSGITSKMTAQSFERIAGANSKVNV
                     AFVGIGNRGLEVLNEFVKTGMVNVVALCDVDLGAPHTAKALADHPGAKTFKDFRKMFD
                     KVRNEFEAVVVATPDHSHFPICMLAISEGKHVYVEKPLTRTFYESELLMNAAAGHKNV
                     ITQMGNQGHSEANYFQFKAWKEAGIIKDVTRITAHMNNPRRWHTWNPKISKFPDKESI
                     PDTLDWDTWLSAVPYHDYNSKFHMGEWRSWYDFGMGALGDWGAHIVDTAHEFLELGLP
                     EEINPLKLDGYNQFFYPMASTIEFKFPKRKKMPPVTLTWYDGVDNIPPVPAGFGATEL
                     DPNIPQVAGWTIQPAKLNPGKEIYSKNLTFKGGSHGSTLSIIPDEKAKEMEKKLPAVP
                     KSSSNHFANFILACQGKEKTRSPFSVSAPLSQVFCLGVIAQQTNRKLIFDRKTKQITN
                     DPFANALLAGVPPRKEWYEFYKL"
     gene            9505..10860
                     /locus_tag="PLN75_03420"
     CDS             9505..10860
                     /locus_tag="PLN75_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRG03201.1"
                     /translation="MKKIIITVAIIIICVTSYAQQPVWQSLFNGKNLKGWTKINGTAE
                     YKVIDNTITGISKPGTPNTFLRTDKMYGDFILEFDFRVDDGLNSGVQFRSNSLKEYRN
                     GTVHGYQFEIDPSSRAWTGGVYDEARRGWLYPMTYNPAGQKAFKKGEWNTARIEAIGS
                     NIRTWVNGIYCADLLNNMTETGFIGLQVHSIGNNQELAGKTVAWRNIRILTTEPEKHK
                     ISAKEEIVQINTIANTISKRETEEGWKLLWDGKTTNGWRGAKMDGFPEKGWRIDDGIL
                     KVLKSDGGESTNGGDIVTMRPYKNFMLKVDFKITEGANSGIKYFVDTELNKGQGSAIG
                     CEYQILDDTKHPDAKLGVNGNRTLGALYDLIPPAANKPFRGGFFNTALIIVKGNHVEH
                     WLNGVKIVEYDRNNQMWDALVDYSKYKDWPNFGNAEQGLILLQDHGDEVWFQNIKIRE
                     L"
     gene            10992..12173
                     /locus_tag="PLN75_03425"
     CDS             10992..12173
                     /locus_tag="PLN75_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRG03202.1"
                     /translation="MKKLALILIFLPLFSFSVISQESEIENIIHSVRENYVPDKRICI
                     YEVTSQINENSLVLKGRISDSNVYQQLLQTIKSRGIAFTDSIQLMPESTIGLKHWGIV
                     PLSAIFIRSAPDFTAEITTQATLGTPVKILDKRAGWLLIQTPDKYIGWTNTTIRMLDK
                     NELSVITKKRRVIVTDFYSTIYKSPSGKSERIMEVLKGNILTLENEKNHLEFSQVSLP
                     DGKKGFIPKKSVIKFSEWQKSIRLTGENVVETAKDYLGLPYLWGGTSPRGFDCSGFTK
                     TVYFMYGKILPRDASQQYFIGEKIDISEGYGKLQKGDLLFFGRKSVSNPNEHIIVHVA
                     IYAGGKKFIHSSGYVRIDSLDPQSEDYDEYNAGRLLGARRIIGTNPENVESIFLNNWY
                     N"
     gene            12218..13363
                     /locus_tag="PLN75_03430"
     CDS             12218..13363
                     /locus_tag="PLN75_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRG03203.1"
                     /translation="MSQNRRNFIKSLAIAGGLSMMDLSVLAEKNARQETSKASRTAKM
                     KLSWFPYNLQLKHAFNISNYSRKTTPVVLVTLEYDGLTGYGEAALPPYLGESQPSVVA
                     FLEKIDLSGFSDPLQMDEILHYVDSVAENNTAAKAAVDIALHDLAGKIIGKPWYQLWG
                     LNIDKIPDTTFTIGMDTEEILRQKVKEVEGQYKILKIKLGGKDDRKMVETIRSVTALP
                     IAVDANQGWKDKHQALEMIHWLKEKGIVMIEQPMSKYLPDDIAWLTAESPLPIFADES
                     IQRLHDVVRTKGVFSGINIKLMKCTGMREAWKMRNLAQSLDMKVMIGCMTETSCAISA
                     AAQLAPGMDFADLDGAQLIQNDAFSGAELINGKVKPLNLPGIGVKPV"
     gene            13498..14367
                     /locus_tag="PLN75_03435"
     CDS             13498..14367
                     /locus_tag="PLN75_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRG03204.1"
                     /translation="MTQRKATQIIRGQHAVDGAGVHLRRVVGLRNVHDFDPFLMLDGF
                     DSLDPNDYIKGFPWHPHRGIETVTYLIKGEIIHADSLGNRGTIRDLECQWMTAGSGII
                     HQEMPQPSERMLGCQLWVNLPAKDKMTLPAYGDITQEDVEIVEEENALVRIISGSYKG
                     RSGAFQGEYIKVKYLDVELAPNATWSYSETPNKETLFIYLLDGTLAPDENLSAFENKA
                     CAVLFTSDNPESTENDRITVKSGGEGARFILLAGKPLREPVSWGGPIVMNTREELNLA
                     FEELDNGTFIKHK"
     gene            14639..15568
                     /locus_tag="PLN75_03440"
     CDS             14639..15568
                     /locus_tag="PLN75_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRG03205.1"
                     /translation="MNLIKSFLLLFLISLALHVQAQKTDRKLTSLIEREVTGFNGTAG
                     VYVKNLKTGKEATFNADTVFPTASTIKVPIMVGVFSKIEKGELDYFKPVIYTSDKQLY
                     KGDDGILSSLKDSTKISLSIVQMLSITTSDNSASLWLQELAGTGTEINRLMHDFGLEN
                     TRVNSRTPGREANREMYGWGQTTPREMASLLVKIRNGAIISRSACEEMYRILGTIYYD
                     DRALSQIPPTVNTASKQGMVSAARSEVVLVNAPHGDYVFAVYTKNQKDTGWEKSNEGR
                     MLIVRISSLLWNYFEPDYGWKPAPGHEKFDGND"
BASE COUNT         4590 a         3129 c         3477 g         4542 t
ORIGIN      
        1 aagaaactta atttttaaaa ttatatataa aatgattaaa gtaggtatta atggttttgg
       61 ccgcatcgga cgtttggttt tccgtgctgc tcagcaaaga aatgatatcg agattgttgg
      121 aatcaacgac ttgataagtg tggattatat gtcatacatg ttgcgctatg atacagttca
      181 tggtcaattc aaaggaaccg tggaagtaaa agacggtcac ctggtagtga acggtaaaac
      241 aatccgcgtt acagccgaaa aagatccttc aaacctgaaa tggaacgaag ttgaagcaga
      301 atatgtagta gaatcaaccg gtttattcct gacaaaagaa actgccgaag ctcacatcaa
      361 agctggtgca aaacgtgtag ttctctcagc tccttcaaaa gatgataccc ctatgtttgt
      421 ttgtggtgtt aacaccgata cttacaaagg tcaagctatc gtttctaatg catcatgtac
      481 taccaactgc ttagctccaa tcgctaaagt tatcaatgac aaattcggaa tggttgaagg
      541 tttaatgact actgttcatt caactactgc tactcagaaa acagttgacg gaccttctgc
      601 aaaagactgg agaggtggac gtgctgcttc cggcaacatt atcccttcat ctaccggtgc
      661 cgcaaaagct gtaggtgtag ttatccctga attgaacaaa aaactgactg gtatgtcttt
      721 ccgcgttcct acattggacg tttctgtagt tgacttgact tgtacgcttg caaaacctgc
      781 aacttatgca gaaatctgcg ctgcaatgaa agaagcttct gaaacatacc tgaaaggtat
      841 tctcggttac accgaagaag aagttgtttc atcagacttt atcggcgatg cacgtacttc
      901 tatcttcgat gcaaaagctg gtatcgcttt gactgacaaa ttcgtgaaac tggttagctg
      961 gtatgacaac gagtggggtt attcaaacaa agtgcttgac cttatcgctg taatggacaa
     1021 agtaaaataa gaacaccctt tgccccaaaa ggggaattgg gagaaactcc gaagatttga
     1081 aattcttcgg agttttttat tttttaaaaa tattaaatca tatttgaata atttctaatt
     1141 ttgcaaatta cctattatta tacctaaatc cccctttagg ggttaggggt cattttttat
     1201 gaattcattc gccgccatag atttcgaaac cgccaatcag catgctacaa gtgtttgcag
     1261 tgtgggaatt gtagttgttc gtgaaggtga aattacggag aagttttaca gtttgataaa
     1321 accggaaccg gagttttatt cgtactggaa tgtacagact catggaatta ctttggaaga
     1381 tacgcagttt tcacctgtat ttccgcatgt ctgggagcag atcgaaccaa tgattgaggg
     1441 actgccatta gtggcgcaca acaagggatt tgatgagcgt tgcctgaagg aagttttccg
     1501 gatgtatcag ctggattatc ccgattatga atttcactgc tcactgcaaa tggccagaaa
     1561 acatctcaaa ggactcctgc caaattaccg gctgcacacc gtttctgagt tttgcggatt
     1621 tgaactgaaa aaccaccatc acgccctggc agatgccgaa gcctgtgcgt ggattgcccg
     1681 gtattttcaa taaaaaaatc cgataattcg tgataattac cggatttttt tttgaatttt
     1741 cttcaatttt acttttccac tggatcattc ttaacagagc aatctttgct tcgaagtggg
     1801 aaaagtttct gatgaaactc accctgattt ttttcgtatt gattagttta aaagattggt
     1861 ttcttagatt tggttcacac tgtgctatgc tgtactgtgc tgatgcaaag ataaggtaaa
     1921 taaataagaa tttcagaaat aaatatatgt tttacaacat attatacaag aaaatgaaga
     1981 ggaaggatgt taattattaa ttaatttaat aaattgacgg gaataaaata aaagaaataa
     2041 gggccagatt ggaggtgaga gttattcatc tgcttttaat aataacctgt tgagcagatt
     2101 gtaagctttg taaattccaa gctcgccggc tttgctcaga tcgagtttgg cgggttcttc
     2161 ttcgttgagg aatacgtagg agaggcctct gccaaagtaa gcttcggaga agttgggatc
     2221 aatttcgatg gctttgctga aattggttac cgaaccgcga tagtctttct ggctagccag
     2281 gaaattgcct ttgttgtaat aagcgaacgt aaaatcaggg ctgagcgcta tggctttatc
     2341 gtaatcttcg ataattcgga ttttgttgat tttattgcgt tcttctgtca tcgaaaacgc
     2401 ttcagcaccc gtttcctgtt tgtcagcgtc agatgtattt gtttcaaatt ccatctgctt
     2461 gatgcgtacg ttgccacgca tgaagtaggc cagcgtcaac tgttcatcca acgcaatagc
     2521 tttgttcagg tcttccagcg cactgctgta gtcctgcacc gattcaaatt cgatggctct
     2581 ggcaaaatac aggtcagcat ccgagttctt ttgtcttatt ctgtccgaca gtttttcaat
     2641 ctgctcgaag tgcgactgta catttccggc gctcattatc ttgctgttga cagctatttt
     2701 cagttttccg ttgtatttgt ttttccggtt gaaattcagt atcagatcgt ggaaacggtt
     2761 ggtacccgac agtaccgaat ctacatcttc aaatgtgagg tcggcatttt tgatgtaagt
     2821 gagttcggcg tagttattct gcacttttcc tcgcaggtta tcgttgtagg ttgttttttt
     2881 gaatttatct tcagactgtg ccgatagttt atcggcaaga cttttagctt gcgtcaccag
     2941 ggcagctgag gttgttactt tagcgatttg atttttgggt tcgagttgtt gttttctctt
     3001 gtaataatcc ttgttgttaa caatctcgaa aacaagtgat ttataataat ttgaagttcg
     3061 tttgtcacct ttcttgttgt atgcttctgc cagcgcatag taagcgggag cgtagtattt
     3121 atatttggtc aggatggatt caaaatcggt tattgtttta tcgagcatac ccaattcaag
     3181 ctccagatgg gctttctgga ggagtgcctc gtagttttgc gggtccattt cgagcacccg
     3241 ttccaggtcg gtgagcgcat tgtttttatc gcccaggtta tttcggagca aagcacggtt
     3301 gtagtaagcc aggctgttgg agcggtcgag tagtatggca aaattataat cgttgagcgc
     3361 ctggttgaaa ttatattttt tcacgttcag aatgccccgg ttaatataat cgcctacaaa
     3421 ttccgaacct ttttcaatgg ctttgctata atcttcgagt gctccctttt catcgttgcg
     3481 caccatcctg atgaatgccc gtatgccgta agcgacgggt tttgagggct cattttcaat
     3541 agcattgtca aatgcattca gtgccgaagt agtgtcgttc agcgccagat agagcctgcc
     3601 catttccacg tcggcatttt cagatttcgg attaattctt ttgagctctt tcaggttgag
     3661 tatggctccc tgcagatcat ttttcttctc tttgatgtgt gcaagcagtt tgtatgaaag
     3721 ttccatggtg ctgtcgattt caatgttttt tcgcaccatt tcttctgcac ctgtggtatc
     3781 gttatcagaa agcattatcc ggcctatttc atattctatg ccttttgctt tcgggtttat
     3841 ttttttgaaa tatttcaaat cttcaatggc tcccttgtaa tccctgtttt tctctttggt
     3901 ttcaatccgg ttagccaggt agtcggagtt gtcgggatca aattcgagcg ctttggtgaa
     3961 atcgtgaatg gcttcggagt atttttcgag atttttacgg gcaaatcctc tcgtgtaata
     4021 tgcgtaaggg ataaaagggc tgagtctgat agcttcattg caatcctgct ccgaacccag
     4081 atagtcgccc aggttgatct tagccatagc cctgtacata taaggttcag gcaggtaggg
     4141 tttcactttg attacctgat tgaaatactg tatggaaagt atataatctt caaaatagag
     4201 cgcatttcgc cctatactca tgatgcggtc ggtattccat tgcccgaaag agcactgaat
     4261 actcagcaaa agcagtattg aaaccagtaa ttttttcaat tcgatttttc agataaaaat
     4321 ggttttttta ttcgcagcct gcttcagaac tccactcgga tgaaactttg aaatcgtcgg
     4381 ttactttgta tcccaactgt ttgtcggcca gtactttctt catgtagttg gcccagatag
     4441 gcaatgccat ggctgctccc tgaccctgcg ccatggaatc aaaacgtatg gagcgttctt
     4501 cgcctccaac ccatactccc gaaaccagtt tgggagtata gcccatgaac catccgtcgg
     4561 aattgttgtt ggtagtacct gtctttcctc ccatgggcat tgtcagtccg taaactctgc
     4621 gaacgcggct gccagtacca tcatcaataa cacttcgcat catgtaaatc attttgaaag
     4681 ctgtttcttc gctgaatatt tcttccattc tgggagtaaa actgccgata atattaccgt
     4741 tagcatcttc tatatgagac acatacaacg ggtcaacgcg gattcccctg gaaggaaatg
     4801 cggtgtatgc atccaccatt tcagctaccg aaacttccac aggtcccagt gccagtgata
     4861 ccaccggatc tattttacct tttattccaa aagaatgcat gagttttaca agcgcttcgg
     4921 gagtaaactg actcatcagc cttgcagaaa tccagttatt cgactctttc aatccccagc
     4981 gaagggttac catttcgcct atccttcggg aagaggcatt tctcgggctc cagggtccag
     5041 aggaggtcat gattgtaatc ggttcattca acaccatatc gcagggcgac atgccttctt
     5101 ccatgcaaag tgtgtacaga tagggtttca cggtcgatcc gatctgacgg cgtccgagtg
     5161 tgaccatatc atactgaaat gcagtgaaat cgggtccgcc tacataagct ttcacgtgtc
     5221 cgtctgccgg gtccatagac ataaatccgc aacgcaggaa atgcttgtga tagcggatgg
     5281 aatccatcgg agtcattacg gtgtccaccc aaccattgta tgtgaataca cgcatttcgg
     5341 tttttgtgtt gaaagatttg tcgatttctt ctttcccggc tccttctttt tttagttttc
     5401 gataacgttc actttgatta acagcacttt gcataataga cgaaacctca gctgtggtca
     5461 gatctctgga aaagggagcg tatttccgtc cttttttctc acggaaaaat tgtttctgaa
     5521 tgctttgcat gtgttgatct accgcttctt cggcgtatcg ttgcattctt gaatcgatgg
     5581 tagtgtaaat cttcagtcca tcggtgtaga tatcgtattt tgaaccgtcg gattttttat
     5641 tcttttcaaa gaaaccatat agcggatcat tttcccacga ccatttatcg ttttcatagg
     5701 tttctttttg ccagtcggca taatcgcttt tcaccggttt tttggcagtg agtactttcc
     5761 gaaggtattc tctgaagtag ggtgcaagtc cttccttgtg atctacccgt ttataatcaa
     5821 gtatcagggg taattttttg atggagtcac tttcgttttc ggttaggaat cctgcttttc
     5881 gcatctgatc caaaaccaca ttcctgcgcg atgatgatcg ttcgttgtat ctcaggggat
     5941 tgaagtagga ggggttctta cacattccca ccagtgttgc tgcttcctga atgttgagct
     6001 tatcagcggg tgagttgaag taaacgtgtg aggctgattt tattcccacg gcattgttca
     6061 gaaaatcgaa ctggttgaga tacatggcta tgatctcttc tttggaatac atcctttcga
     6121 gttttacggc tattacccat tcgattggtt tctgaaaggc acgttctata atattgcctg
     6181 ctccgggtga gtagagttgc ttggcaagct gctgggtgat ggtgctgccg cctccggagt
     6241 tggagcgggt aagtacaccg aatactgctc tgataaggcc gcgaaggtcg atacccgaat
     6301 gcttgtagaa ccggatatct tcggtggcaa tcagcgcgtt gatgatgtct ttatctattt
     6361 cgttgtagtt gatgggcacg cggttgtcgc gtcctttgta gtaccttcca agcagttgag
     6421 catcagatga atatatttca gtagccgatt tattgatcgg attctgcaat tcgcctattt
     6481 ccggcagata accaatccat ccgatgctga tcagaaaaaa taatagtacg actacagcca
     6541 gcccaattcc aaagattttc caaaatactt tcttgaaccc gatgcgcctt tcctccgtta
     6601 atgccatatt cctgttgctt tcttaatatt ttatatctta caaaattacg tttatttttt
     6661 taattgagtg atttggcaga ccgatttgaa ctttttttaa tagttttggt atattttctc
     6721 acacgtacat catatcggcg attatcttat ccgatatatg aatgccttcg gatgttaaac
     6781 ggagaaagtt gaattttctt acaattttcg aatcttgaat atacttttcg gccgattgca
     6841 aacaaaaatc atgcagtttt tctccaaaat tatttttcaa tgattccaga tcaatccctt
     6901 cgattgttcg catcgaaacc atcacaaaat cgttgtattt atccggcagg gtaagtattt
     6961 ctttttcaaa aaaaggttga ttttctgaaa tgaatgagca gtattttttg ttggatgata
     7021 cattccactg gcgtgaattg ccatcataag agtgtgctgc aggacctatg ccaaggtaag
     7081 gtctttgctt ccagtaggct gaattgtggc gtgaccgatg ccccggttta gcaaaattgg
     7141 aaatctcata gtgttcgaag ttcgattttg aacaggtttc gacgaggtaa ttgtacattt
     7201 cgatcatggt gtcgtcgtcg gttggaataa tttttccgga tttcatccgg tgccagagcg
     7261 gagtaccctg ttcgtaagta aggccataag ctgaaatgtg ctgtacgcga agttttacag
     7321 ccatatcaac ctgcattttc catgattcca ggctttggcc tggtaatccg aaaatcaaat
     7381 cgatgcttat attttcaaaa ccggtctgat gagccacgtt gatactgtcc agagctgccc
     7441 gggccgtatg gcgtcggttg atagctttca gctgtaggtc atcaaaactt tgtatgccta
     7501 tgctcaggcg atttacacct gtttttatga gctctttcag ataaccggct gataaatcat
     7561 ccggattggc ttcgattgta atttcagcat cttcatttac agaaaaaaaa gaactgacag
     7621 cattcaaaag gtttttaata tcagctgctg ataatgtaga gggtgtgccg cctccgaaat
     7681 aaatggtttc gacttcctcc gagagatatg tttttctgat ttcaatttcg cggagcatgg
     7741 catttacaac atcttgttgc aacgaaaaat ccgtttgagt gtagaaatcg cagtatgtac
     7801 accgggtttt acagaatgga atgtgaaaat agatacctgc catggttgtt ttttttgata
     7861 gatttacaaa aatacgatta gttcgatgat tgataaagct ataaaacatt ttattcgtaa
     7921 aatttttact atttttggga aatgaatatt ttttttgaaa aaaaccgttt tataacatga
     7981 caacacgaag agaatttatc aggagtgctg ctctggcatc ggcagggctg gctttcagcg
     8041 gaattacttc caaaatgaca gcgcagagtt tcgaacggat agcaggtgcc aacagcaaag
     8101 tgaatgtcgc ttttgtgggc attggtaacc ggggcctgga ggtgcttaat gaatttgtaa
     8161 aaaccggaat ggtcaatgtg gtagccctgt gcgatgtgga tttgggcgct ccccatactg
     8221 ccaaagcact ggcggatcat cccggagcca aaacattcaa agatttccgg aaaatgtttg
     8281 acaaagttcg caacgaattt gaagcagtgg tagtagctac acccgatcat tcacatttcc
     8341 ccatttgcat gcttgccata tccgaaggaa aacatgtata cgtcgaaaaa ccgcttacac
     8401 gtacgtttta tgagtcggaa ctgctgatga acgctgccgc cgggcataaa aacgtgatta
     8461 cacagatggg caatcaggga cactccgaag ccaactattt tcagtttaaa gcctggaaag
     8521 aagccggaat cattaaagat gtcacccgaa ttacagcaca catgaacaat ccgcgccgct
     8581 ggcatacctg gaatccgaaa atttcaaaat ttccggacaa agaatccatt cccgatacgc
     8641 tcgactggga tacatggctt tcggcagtgc cttaccacga ttacaacagc aaatttcaca
     8701 tgggagaatg gcgctcctgg tacgattttg gtatgggcgc actgggcgac tggggtgcac
     8761 acattgtgga tactgcacac gaatttctgg aactgggatt gcccgaagaa ataaatccgc
     8821 tcaaactgga cgggtataac caatttttct atccaatggc aagtacgata gaatttaaat
     8881 ttccgaaaag aaaaaaaatg ccacccgtaa ctttgacctg gtatgatggt gtggataata
     8941 tacctcccgt gcctgccggt ttcggagcta cggaactcga tccgaacata ccacaagtgg
     9001 caggatggac cattcaaccg gcaaaactga atccgggaaa agaaatttac agcaaaaacc
     9061 tgacattcaa aggagggtcg cacggcagca cgctgagtat tattcccgac gaaaaagcca
     9121 aagaaatgga aaagaaattg cctgcagtgc ccaaaagttc gtctaatcac ttcgctaact
     9181 tcatcctggc ttgccaggga aaagaaaaaa cgcgctcacc tttcagtgtc agtgcaccac
     9241 tgagccaggt tttctgcctg ggagtgattg cacagcaaac gaaccggaaa ctgattttcg
     9301 acagaaaaac caaacaaata accaacgatc cgtttgccaa cgctttgctg gcaggagttc
     9361 ctccacgtaa agaatggtac gaattttata aactttaaat taccggtttt agcatgaaac
     9421 gagcatgaac acggtggttc aacaaagatg atgaaaatct gacatgcgag ttccatgcga
     9481 caactaaaaa acaattattt acgcatgaaa aaaataatta ttacagtcgc aataatcata
     9541 atttgtgtga cttcatacgc ccaacaaccc gtctggcaaa gtttattcaa tggaaaaaac
     9601 ctgaaaggat ggacaaaaat aaacggaact gccgaatata aagtgattga caacaccata
     9661 acaggcattt caaagccggg aacgcccaat acattcctta gaacagataa aatgtacggc
     9721 gattttatcc ttgaatttga tttcagggta gacgatggac tcaattcggg tgtacagttc
     9781 agaagcaaca gcctgaagga atacagaaac ggtacagttc acggttatca gtttgagatt
     9841 gatccttcat ccagagcctg gacaggaggc gtgtatgatg aagcccgcag aggctggtta
     9901 tatccgatga catataatcc ggcagggcaa aaagccttca aaaaaggaga atggaacacg
     9961 gcacgcatcg aagccatcgg aagtaatatc cgcacctggg taaacggaat atattgtgct
    10021 gatttactga ataatatgac agaaacggga tttatcggct tgcaggtaca ttctattggc
    10081 aacaatcagg aacttgcagg taaaacagtt gcctggcgga acatacggat actgactacg
    10141 gaaccggaaa aacacaaaat atcagcgaaa gaggaaattg tgcaaataaa tacgatagcc
    10201 aacaccattt cgaaacgtga aactgaagag ggttggaagt tgctttggga cgggaaaacc
    10261 accaacggtt ggcgaggtgc caaaatggac ggatttcctg aaaaaggctg gagaatagac
    10321 gacggaatac tgaaagtgct gaaaagcgat ggaggggaat caaccaacgg cggcgacata
    10381 gtgacaatgc gtccgtacaa aaatttcatg ctgaaagtgg atttcaaaat caccgaagga
    10441 gccaatagcg gtataaagta tttcgttgat acagagctta ataaaggtca gggatcagct
    10501 atcggatgcg agtaccagat tcttgacgac acgaaacatc cggatgctaa actgggtgta
    10561 aacggcaaca gaacacttgg agcactctac gatttgattc cacccgctgc caataagcca
    10621 ttccggggcg gatttttcaa tacagctttg attattgtga aaggaaatca tgtagaacac
    10681 tggctcaatg gagtgaaaat agtggaatac gaccgaaaca accaaatgtg ggatgcgctg
    10741 gtggattaca gtaaatataa agactggcct aactttggga atgcggagca gggattaatt
    10801 ttgcttcagg accacggaga tgaagtatgg tttcagaata taaaaatcag ggaactgtaa
    10861 accaaaaaag gaattaattt gataaaaaag ttggaagtta ttacagattc cggctttttt
    10921 ttatatggag caaattgaat gctcttaaat taaaatcgtt atatttgcac gaagataaaa
    10981 gtcagatgga aatgaaaaaa ctagccctta ttcttatttt tcttccactt ttttcttttt
    11041 cggttatctc gcaagagtct gaaatagaaa atattattca ttccgtaagg gagaattatg
    11101 tgccggacaa acgaatttgc atctatgaag ttacttccca aatcaacgag aactcattgg
    11161 ttctgaaagg gagaatttca gattcaaacg tttatcagca gctactgcaa acgattaaaa
    11221 gcagaggaat tgcattcaca gacagtattc agttgatgcc cgaaagcacc atcgggttga
    11281 aacactgggg cattgtgcct ctttcggcca tttttatccg ctcagcaccc gattttactg
    11341 cggaaataac cacccaggcc acactgggaa ctcctgttaa gatactggat aaaagggcag
    11401 gctggctact tatccagact cccgataaat acatcgggtg gacaaataca accatacgaa
    11461 tgttggataa aaatgaattg tccgttataa ctaaaaaacg gagagtaatt gtaactgatt
    11521 tttattcaac tatttataaa tctccttccg gaaaatcgga gcgaataatg gaggttttga
    11581 aaggaaatat actgacactt gaaaatgaaa aaaatcacct ggaattttca caggtatcat
    11641 tgcccgacgg caaaaaagga tttattccga aaaaatcggt aatcaaattc agcgaatggc
    11701 aaaaatccat ccggcttaca ggcgaaaatg tagttgaaac cgccaaagat tatctgggtt
    11761 tgccttatct ctggggaggc acttccccgc ggggttttga ttgcagcgga tttaccaaaa
    11821 ccgtatattt tatgtacggt aaaatcctgc ccagagatgc ttcacagcaa tactttatcg
    11881 gcgagaaaat tgacatttcc gaaggttacg gcaagttgca aaagggtgat ttgttgtttt
    11941 ttggacgaaa atcggtatca aatcccaatg agcatatcat tgtccatgtg gcaatttatg
    12001 ccggcgggaa aaaattcatt cattcatcgg gatatgttcg gatagacagt ctggatccac
    12061 aaagtgaaga ttatgacgag tacaatgccg gtcgacttct gggtgcacgc cgtattatcg
    12121 gaacgaatcc tgagaatgtg gaaagcatat tcttaaataa ttggtataac tgatatattt
    12181 acagcttatt aaatagtagt atttaaatgt aaacattatg tcgcaaaatc gaagaaattt
    12241 tattaaaagc cttgcaatag ccggtggttt gagtatgatg gatttgtcag tgctggcaga
    12301 aaaaaatgcc cggcaagaaa cctccaaagc atcacgaact gctaaaatga aactgagttg
    12361 gtttccctat aacctgcagc tgaaacatgc attcaacatc tccaattatt cgcggaaaac
    12421 aactccggta gtactggtaa cgcttgaata cgacggatta accggatatg gtgaagcggc
    12481 gctacctcct tatttaggcg aatcgcagcc ttcggttgtc gcttttttgg aaaaaataga
    12541 tttatccggt ttttcagatc cgctgcagat ggatgaaata ttacattatg tggattctgt
    12601 tgccgaaaat aatacggctg ccaaagcagc tgtggatatt gcattgcacg atttggccgg
    12661 aaaaataatc ggaaagccgt ggtatcagct ttgggggctg aacatcgaca aaatacccga
    12721 tactacattt acaatcggga tggacaccga agaaatttta cgtcagaaag tgaaagaagt
    12781 ggaaggtcag tataaaatat tgaaaataaa actgggtgga aaagacgacc ggaaaatggt
    12841 ggagaccatt cgttccgtta ccgcattgcc tatagccgta gatgccaatc agggctggaa
    12901 agacaagcat caggcgcttg agatgattca ctggctcaaa gaaaaaggaa ttgtgatgat
    12961 agaacaaccg atgtcgaaat atctcccgga cgatatagcc tggcttaccg cagaaagtcc
    13021 gcttcccatt tttgccgacg aatcaatcca gcgattgcat gatgtggtga gaacaaaagg
    13081 tgttttctca ggcatcaata tcaaactgat gaaatgtacg ggtatgcgcg aagcctggaa
    13141 aatgcgaaat ctggctcaat cactcgatat gaaagtgatg ataggctgca tgactgaaac
    13201 ttcttgtgcc atatcggctg ccgctcaact cgctcccggg atggattttg ccgatctgga
    13261 cggcgcacag cttattcaaa acgatgcatt cagcggagcc gaacttatta atggaaaggt
    13321 gaagcccctg aacctgcccg gcattggagt aaaaccagtt tgacactgat tgtcatcccg
    13381 aacgaaacgc agtggagtga gggaactcac agacaagtcg aagcgaaacg cagtatagtg
    13441 aagaagcaaa aaaacatata taaaaaaaat cttacatcaa actaacgtta aattatcatg
    13501 acacaacgaa aagcaacaca aattatcagg ggtcagcacg ctgtagatgg tgcaggagtt
    13561 catttaagac gggtagtagg gttaagaaac gtgcacgatt ttgatccgtt tctgatgctc
    13621 gatggctttg attcgctgga tccgaacgat tacatcaaag gctttccctg gcatccgcat
    13681 cgtggaattg aaacagtaac ttatctgatc aaaggggaaa taatccatgc cgacagcctg
    13741 ggaaatcggg gaacgatcag ggatttggag tgccaatgga tgacagccgg ctcgggaatc
    13801 attcaccagg aaatgccaca accgtctgaa cgaatgctgg gttgtcagct ttgggtaaat
    13861 ctgcccgcaa aagataaaat gaccctgcct gcttatggag atattacgca ggaagatgta
    13921 gagattgtag aagaggaaaa tgcgttagtt aggattattt cgggtagtta caaaggtcgt
    13981 tccggtgcct ttcagggtga atacataaaa gttaaatacc tcgatgtgga acttgctccc
    14041 aatgcaacct ggagttattc tgaaactcca aataaagaaa cattattcat atatttgctt
    14101 gatgggacat tagcacccga tgaaaattta tcagcttttg aaaataaggc ttgtgccgta
    14161 ttatttactt ctgacaatcc ggaaagcact gaaaatgatc gcattacagt aaaatcgggt
    14221 ggggaaggag cgcggttcat tttgctcgcc ggcaaaccgc tccgcgagcc cgtttcgtgg
    14281 ggaggaccca ttgtcatgaa tacgcgggaa gaactgaatt tagcatttga agaactggat
    14341 aacggcactt ttataaaaca caaataaaaa atcaacttac caattaaaaa aagtcaattt
    14401 aatatgtaca gaaagaagaa accacttacg ggttaatcgt aagtggttga ttgtcaagtc
    14461 ggggtatcgg gattcgaacc cacgaccctc ccgattcaaa tcgggatgtg ctaaccggac
    14521 tgcttttaat aataaaaact tctttaaaat cagaaataaa aacaacctga aatcactggc
    14581 aatctgaaac gtactacgat aattttgagt gaaataaaat attcttacat aaaatccgat
    14641 gaatttaatt aaatccttcc tccttctttt cctgataagt ttagcactcc atgttcaggc
    14701 gcaaaaaacc gatcgtaaac tgacctcact catcgaaagg gaggttaccg gttttaatgg
    14761 aactgccggt gtatatgtga aaaatctgaa aactggaaaa gaagcaacat tcaatgctga
    14821 tacggtattc cctaccgcca gtacaattaa agttccgatt atggttggag ttttcagtaa
    14881 aattgaaaaa ggtgagctgg attacttcaa accagtaatt tatacttctg ataaacaatt
    14941 gtataagggt gatgacggaa tattgtcttc attgaaagac agtacaaaga tatctctgag
    15001 catagtgcag atgttgagta ttactacgag cgacaacagt gccagtttgt ggcttcagga
    15061 actggcagga accggcactg aaatcaaccg actgatgcat gattttggac tggaaaatac
    15121 aagggtaaac tctcgtacac ccggcaggga agccaatcgg gaaatgtatg gctggggaca
    15181 aactactccg cgcgaaatgg catcgctact ggttaaaatc cgaaacggag caattatttc
    15241 ccgatctgcc tgcgaagaaa tgtacagaat ccttggtaca atttattacg acgaccgtgc
    15301 gttaagccag attcctccaa ccgtcaacac agcctcaaag caaggaatgg tcagcgctgc
    15361 acgttcggag gtggttcttg taaatgcacc tcacggcgat tatgtttttg cagtttacac
    15421 aaaaaatcag aaagacaccg gttgggaaaa gagcaatgaa ggacgaatgc tgattgtacg
    15481 tatatcctct ttattgtgga attattttga gccggattat ggatggaaac ctgctccggg
    15541 acatgaaaaa tttgatggga acgattaatt gagtgttttt tcggaaattt taatcatctt
    15601 atccatttaa tcgattgttt aaatttcaat caaacataaa ttgtaaacaa tataattgat
    15661 attttatgat ttttcagaga acgttttttt ttcaatgttc gtttctctgt tttaaatatc
    15721 aggcaatcaa tctattgc
//
LOCUS       DAORKP010000036        15487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_280_length_15487_cov_7.846423, whole genome shotgun sequence.
ACCESSION   DAORKP010000036 DAORKP010000000
VERSION     DAORKP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_15487_cov_7.846423"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLN75_03445"
     CDS             <1..663
                     /locus_tag="PLN75_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03206.1"
                     /translation="EELYAEATEKKADVVLSLLENDKNLKEDFVGYGDIVYSKDFILK
                     DLIGFGYENNPGALFVRVTGNLYKKSLLKLNRINFVDTLTTTPEDVIFQIKSILRSEN
                     LALIKKQFYFHQKHTDNEGNKGSYVSFEKRGAGMAEIYSLLIREKVFDYFRHYFYKGV
                     TKQFLNALAGTVYPKFSISQFVRGVKLLRSYPFCNEAFAVYSLPPAKHKWLRMLLVKM
                     LK"
     gene            complement(664..1659)
                     /locus_tag="PLN75_03450"
     CDS             complement(664..1659)
                     /locus_tag="PLN75_03450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03207.1"
                     /translation="MSREDGIDVFRNYHISFPFFKIRDAGNWSSETLKMFEAYYRQFG
                     KPDLIHVHSVLWAGYAASLIKEKHNIPYIITEHRGIFGLSCDWAKNQFIEWQDEYMQQ
                     AFSNADLIIPVSEKLIPKISSYLDRPVPMRVISNMVDSAFFYPKYQEKSKETIQAVMV
                     NGFTYVKGYDILFPALDIAFERVDNLRITIVGEDFYGEVFDKQWSQVRHKNRITLAGE
                     KDKFGVRDALWTADFFIISSRVESQSVSTLEALATGLPVVCTEVIPETIVNESNGLRV
                     PVENVEKLSEAIVSMSESYDSYDRLKLSAEIKKVADKKVVSGMLIDAYQSIINSK"
     gene            complement(1845..2792)
                     /locus_tag="PLN75_03455"
     CDS             complement(1845..2792)
                     /locus_tag="PLN75_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03208.1"
                     /translation="MLEKFEIVPFTQTRGWYDLHSLYKPEKIIFLVNNTDNPTIACFA
                     HRKTFLGLSMILIEGEAYKNDRACNLNDVRSFYKHLTESGFPIIESTSGATYDFEYET
                     ALRQAGYLRPVGLFSMPSTKIINLQNPVCYNQNWKRNIRKANENNLYFEVTEKITTKE
                     CEDFLKIYSEMTSRKNLSFGFNSEQLFQLLSDSTHFQLFFVKSSSHERIASILIHKDI
                     HRAGLLYAATSQAALETSASFYMYDKLFEYLTSENFRFFDMEKLIPSDDVVNHVFNFK
                     NGIQGTHIQLNGEWSWYKNSFYRIAMYFVKKFLVKKREL"
     gene            complement(2822..3592)
                     /locus_tag="PLN75_03460"
     CDS             complement(2822..3592)
                     /locus_tag="PLN75_03460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG03209.1"
                     /translation="MIIQPQLTQYNGIWLDYGNELSDNDKIHFEKKAIGNLLVQLNDF
                     GFDYFNQNFPLNFVNWLPFYWQKFSQTTRYTYQLTGINNPEACFEKFSYAKKKQIRKA
                     QKELTVSTKMSAEDFYVHLTAYQTSRRQKMIYSDSFFMNLYNSCKSKNQGEIIAVIDS
                     KSVVHAALFIVWDNLTGYNLISTIHPDYKNSGASTLVVFEAIKYLSDKVQTFDFEGSM
                     DAGIENSFSQFATVQSPYFSIKKYNSLTTRALFPIFKL"
     gene            4027..5688
                     /locus_tag="PLN75_03465"
     CDS             4027..5688
                     /locus_tag="PLN75_03465"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRG03210.1"
                     /translation="MKKFFLFILTILTGGLSPSYAQDKLTGILTNELMRNFEVMKQLD
                     VPVYYISLRVEDSESYNITGSNGAVTQNRYQKGRAMAITMRVGSPAFDNMHYSGNMQN
                     IKRVALPDTDIEEEIKLSLWRALQQAYSEAKDKLQSNFTEQTTRPAEEDKSPDFSIEK
                     ASKYYEKPVPFSDLGFDENEFINKTNELSKVLSDNRDVLINFVNVTSNLTRGYFIDTD
                     GASVVQNNPSIRIYLGGYVMADDGMMLQDYRTYFGKKVADLPDSEKILSDARELSQNL
                     STLKKSPKIDSYTGPVILSGEVSGVFFHEFFGHRVEGARMKSGMDAQTLKKKIGETVL
                     PQRLSVTFDPTRSKYGNSILSGDYKYDDEGVRGQKVEVIKNGVLTGFLMSRIPIEGFS
                     KSNGHGRGVLNAGTETRQSNMIIESTKPVTAKEMHEMFVEELKKRGLKFGYRIEKVSG
                     GLTMTSSASANAFYLNPLIVYRVYVDGRPEEMVRGINIVGTPLNAFSQILAETSEHAI
                     FNGMCGALSGSVPVSAVAPAMLIKELEFQKTGDSQKMDPFILERP"
     gene            5714..7318
                     /locus_tag="PLN75_03470"
     CDS             5714..7318
                     /locus_tag="PLN75_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03211.1"
                     /translation="MKQFVLNNILAVVLFLTVVSFSAAQTNDVLLNAMKTEADRSKAE
                     LKRDSLARPFYISIISGEKDIEFTFNTSFGKVKKELTDTRKSIYAGSQVLVGDYHRTQ
                     NFTSTTNRFTLPVENNEMAIRTAIWMNLDELYKNAARNYVDKMAWLKQENSKADELAL
                     DDFEKRNPTVLIQDIEKPDYDFSKAKQLSLKIGKLLTEKVNQEKLLVDELTTSFNFSR
                     EIVRYYDTEGSIYRYPKQDGRFNITLVGVTEDGEDVGAYRSFRLNNPEELPAFEDLKK
                     AVSDMVNEYKIKYSSEDLDDPYMGPLLIVDGQVETVAKQIVNNLYTSPKTRFSNGNHY
                     QNMPGVRIISKQLSMMINYGKIAQQIPGVKRDLAPIDIQAVVPPDTILLIKDGILKNM
                     LTTRQPVKKFLHSNGNAKNGNSPEGVITFTGNQTYSYPEIKKMLIDEASLQGLEYAYI
                     MNADGKLHRKVNVETGEEIPFTANFNFGQILKTMRRVLGVEDKMTASSDGTVRFPKSI
                     LLEDAELTVYKSNNRLKKNYVTRPEL"
     gene            7453..7860
                     /locus_tag="PLN75_03475"
     CDS             7453..7860
                     /locus_tag="PLN75_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRG03212.1"
                     /translation="MARFQDESGNTSSPISGGIINIIAAGTKIVGKVSVESDFRLDGE
                     VEGDIFGKSKVVIGEKGNLKGSLTCANAEIVGKVTGNIMTSHSLTLRSTAELNGDVKI
                     KSLIVEPNAIFNGTCSMRENAQPQQNTQNQPVK"
     gene            8025..9026
                     /locus_tag="PLN75_03480"
     CDS             8025..9026
                     /locus_tag="PLN75_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HRG03213.1"
                     /translation="MKTTDINYKALVVSENEGTYVQSIQELNTNSLPEGDVLIRVHYS
                     SLNYKDALSATGNKGVTRKYPHTPGIDASGEVVESDSLQFKTGDKVIVSGYDLGMNTP
                     GGFGRYIRVPSSWVVLLPEELSLKEAMVIGTAGFTAGISITRLSELVKPSDGKIIVTG
                     ATGGVGSVALSILSRLGYQTVAVSGKENEFAYLESLGAGEIILRSDFLNLEKKPILSA
                     KYAGGIDAVGGPILENILKSLLPLGAVTTCGSVSSTELNMSVFPFILRGISLIGISAQ
                     NYPMKLRTTLWNRLANEWKPEHLMSIYNEIMLQELPDAIQLILNGKLKGRTLVNLTL"
     gene            9130..10350
                     /locus_tag="PLN75_03485"
     CDS             9130..10350
                     /locus_tag="PLN75_03485"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRG03214.1"
                     /translation="MALANENYLKTPEIYGFDEIEKRINAYRILNPKSELINIGLGDV
                     TLPLIPEVVKAMHLASDELSKTSTFKGYGPPKGYDFLISKIQKNYKTNGIAIDKDTIF
                     VNDGAKSTIGNIGHVLGRDNIIAISDPVYPVYENATIMSGRAGDLNEKQQWSHIVYLH
                     CSEKNNFIPDLPKERVDIIYLCNPNNPTGTAMNRSELKKWVDYAIENHSIIVYDGVYS
                     EFITEPDIPRSIYEIKGAKKVVVEVRTYSKLAGFTGLRCGYAVFPNELTVYTQEGESI
                     PLVKLWNRRNSYYTNGVSYIVQRAAEAIYSIEGERQIKEQISYYMTNAAMLREQLIAL
                     GHEVYGGVNAPYVWMKTPGNSSSWDYFNELLYLKKIIVTPGVLFGKNGEGYIRFSGFA
                     NREMMKLALEKFKT"
     gene            10384..12225
                     /gene="glmS"
                     /locus_tag="PLN75_03490"
     CDS             10384..12225
                     /gene="glmS"
                     /locus_tag="PLN75_03490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRG03215.1"
                     /translation="MCGIVGYIGNRQAYPILIKGLHRLEYRGYDSAGIALINHNNLKV
                     YKAKGKVSELEHFAEQKDISGTIGIAHTRWATHGEPNQKNAHPHYSQSEEIAIIHNGI
                     IENYAVLKEGLMKEGYTFQSQTDTEVLVQLIEYMKKTNNSDLPTAVQLALNQVVGAYA
                     IAVLEKNNPDLIVAARKGSPLVIGIGEDEYFIASDATPIVEYTNKVTYIGEEEIVTIM
                     RNEPLKIKTIGNIEKTPHIQKLEMTLSQLEKGGYPHFMLKEIFEQPKTLNDCMRGRVN
                     VDSDNITLAGFIDNRDKFLNAKRIIITACGTSWHAAQIGMYAIEEFARIPVEVEYSSE
                     FRYRNPVINKDDIVIAISQSGETADTLAAVELAKKHGAFIFGICNVVGSSIPRNTHSG
                     CYTHVGPEIGVASTKAFTAQVTVLTMLAILIGKEKETLSNEEYLKLIKELSLIPDKIS
                     EVLQKNSQIAEMSKTFTYASNCIYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMK
                     HGPIALISNEMPVIAIATQSDTYEKVISNIQEIKARKGRIISIVTEGDEVVRKLSSYS
                     IEVPKTETCLTPLLSVIPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
     gene            12233..14029
                     /locus_tag="PLN75_03495"
     CDS             12233..14029
                     /locus_tag="PLN75_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRG03216.1"
                     /translation="MCGIAMIRLLKPLDYYQKKYDSWQYGLNKMYLLMEKQHNRGQEG
                     AGIATLKLDITPGEEYIWREREEGKNAIQQIFANINEKIEKINGLVDDPAFAKENLPF
                     AGELYLGHLRYSTTGKSGISYVHPFLRRHNWKARTLVMAGNFNLTNVDEIFENLTEQG
                     QHPRDKADTFLMLESLGNKLDNEVQAEYEQIRKRYSNDKIITQKIEEQLDIPSFIRKS
                     EKKWDGGYVICGMLGHGDSFVFRDPDGIRTAFYYQDDEIVVVASERAPLQTVMNVKTE
                     DIRELQPGQIMIIKKNGNVLFDTIRIPSAEIKACSFERIYFSRGSDVEIYNERKKLGA
                     TLAEPILKSIDYDLENSVFSFIPNTAEVAFYGMTQGIRSITGKDPHVEKVLIKDIKLR
                     TFISQNKERNDLAKHVYDVTWNSIRRGKKDNLIVIDDSIVRGTTLKQSILSIINRLEP
                     KKIVVVSSSPQVRYPDYYGIDMSKMSEFCAFRAAIALLKESGRQQLIDEVYQECKSQE
                     NEVKEKIVNYVTKIYEPFTQEEISQKIAEILTPADVECPVDLVFQTVEGLHAACPNHT
                     GDWYFTGNYPTSGGKRLVNHAFINYYEGKEKQ"
     gene            14026..14748
                     /gene="hisA"
                     /locus_tag="PLN75_03500"
     CDS             14026..14748
                     /gene="hisA"
                     /locus_tag="PLN75_03500"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRG03217.1"
                     /translation="MIEIIPAIDLIDGKCVRLTQGDYSQKKIYNENPIEVAKMFQDAG
                     ISRLHLVDLDGAKAQHIVNYIVLERITSKTDLIVDFGGGLKSDDDLRIAFECGASMVT
                     GGSIAVKNPEIFTGWIEKYGSEKIMLGADVKNMKIAVSGWMEDTDIELIPFLETYQKR
                     GISKIICTDISKDGMLEGPSIELYRDIIRKLPDVFLIASGGVSSIADIEILHDCGVPA
                     VIFGKAIYEGKIKLKDLSRFLM"
     gene            14880..15428
                     /locus_tag="PLN75_03505"
     CDS             14880..15428
                     /locus_tag="PLN75_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRG03218.1"
                     /translation="MLKEILSITGKPGLYKLVSQGKNMLIVESLIDGKRIPTYAKDKA
                     VSLGDIAIFTETEEVPLGKVLEKVKSKENGLKASIDSKSENDELRKYMEEVLPEYDKD
                     KVYPSDIRKLINWYNLLIESNITEFVEKEEETEEHPEKELEVKEKGKKDTKPAAAKKE
                     PKVQVKQPKIAQKSMTNRKMGG"
BASE COUNT         5101 a         2963 c         3169 g         4254 t
ORIGIN      
        1 gaagaattgt atgctgaagc tactgagaaa aaagcggatg ttgtgttgag tttactggaa
       61 aatgataaga atcttaaaga agattttgtc gggtatggag acattgtgta ttctaaggat
      121 tttattctta aggatttgat tggatttgga tatgaaaata atccgggggc attgtttgtt
      181 cgggtaacgg gcaatttata caaaaaaagt ttactgaaat tgaatcggat taattttgtg
      241 gatacgctca ccacaacacc tgaagatgtt attttccaga ttaaatccat tctaaggtct
      301 gaaaatctcg ctttgataaa aaaacaattc tattttcacc agaaacacac tgacaatgaa
      361 ggtaataaag gttcttatgt gagtttcgaa aaacgggggg cgggtatggc ggaaatctac
      421 tctttattaa taagagagaa agtatttgat tatttcagac attactttta taaaggggtt
      481 acaaagcagt ttctgaacgc attagccgga acggtttatc caaaatttag catttcgcag
      541 tttgtcaggg gagtgaagct tctacgctct tatcctttct gtaatgaagc ttttgccgtt
      601 tattccttac ctcctgctaa acacaagtgg ctcagaatgt tactggtgaa gatgctgaaa
      661 taatcatttg ctgttaatga tgctttgata tgcatctatc aacatgccgg aaacaacctt
      721 tttgtctgca acttttttta tttcggccga aagtttcaat ctgtcatagc tgtcatagct
      781 ctccgacatg ctgactattg cttccgagag cttctcgacg ttctctaccg gaacccgcaa
      841 accatttgac tcattgacaa tcgtttccgg aataacctct gtacacacca ccgggagtcc
      901 ggtggcaagc gcttcgagag tggaaacgga ctgagactct acacgactag aaattataaa
      961 aaaatctgca gtccataacg catctcttac tccgaatttg tccttttctc ctgcaagagt
     1021 aattctgttc ttgtgtctga cctgtgacca ctgcttatca aaaacttctc cgtaaaaatc
     1081 ttctccaaca atagtaatcc taagattatc aacacgttca aatgcaatat ctaaagccgg
     1141 aaataaaata tcataacctt ttacatacgt aaatccattg accataacag cctgaatagt
     1201 ttctttactc ttttcttgat actttgggta aaagaaagcg gaatcaacca tgtttgaaat
     1261 tactcgcatc ggaactggcc tgtccaggta ggagctgatt tttggtatca gcttttctga
     1321 aacggggatt attaagtcag cgttggaaaa tgcttgttgc atatactcat cttgccattc
     1381 aataaactga ttttttgccc agtcgcatga aagtccaaaa atccccctgt gctcggtaat
     1441 aatatagggt atattgtgtt tttctttaat cagtgaggct gcatatccag cccagagtac
     1501 agaatgaacg tgtatcagat cgggtttgcc gaattgccgg tagtacgctt cgaacatttt
     1561 cagtgtttcg ctcgaccaat tcccggcatc ccgaattttg aaaaaaggaa aactgatgtg
     1621 atagtttcgg aaaacatcaa taccatcttc acgactcaaa atgaactttt tagaaaatcc
     1681 aaatgctttt aaaccgtatt ttctgatcga aattgacaca tttgccaaaa tggatgcata
     1741 tactttaccg gtttcattca gggcctgaac ctgatttctg caaaattgcc ccccttccgg
     1801 taaataccag gaggggagat gcagaacatg taatttctgg cttttcatag ctctcttttc
     1861 tttaccagaa attttttaac aaaatacatg gcaattcgat agaatgaatt tttataccag
     1921 ctccattctc cgttgagctg tatatgagta ccctgaattc cattcttaaa attaaataca
     1981 tggtttacga catcatcgga tgggattagt ttttccatgt cgaaaaaccg aaaattctca
     2041 cttgttaaat attcaaaaag tttatcgtac atgtaaaaag aggcagatgt ttctaatgca
     2101 gcctgagatg tagctgcata caataatccg gccctgtgta tgtctttatg aatcagtatg
     2161 gaggcaatac gctcgtgaga tgaacttttg acaaaaaaca gctgaaaatg agtggaatct
     2221 gatagcagct gaaaaagttg ttcggagttg aaaccgaatg aaagattttt ccttgatgtc
     2281 atttctgagt atattttaag gaagtcttca cactcttttg tggtaatttt ttcagttact
     2341 tcaaaataca ggttgttttc atttgctttc cggatatttc ttttccagtt ttgattatag
     2401 catacaggat tctgtagatt gatgattttt gttgagggca tactgaataa gcctaccggc
     2461 cgcaggtatc cggcctgacg cagtgcggtt tcgtattcaa aatcgtaagt tgctccggag
     2521 gttgattcta taatcggaaa acccgattcg gtgaggtgct tataaaaact cctgacatca
     2581 ttcagattgc acgcccggtc atttttgtat gcctctcctt caattaaaat cattgataaa
     2641 cctaaaaaag tttttcggtg agcgaagcat gctatcgttg gattatcggt gttgtttaca
     2701 aggaaaatta ttttttcagg tttgtaaagt gaatgtaaat cataccagcc cctggtttga
     2761 gtgaatggta ctatttcaaa cttttccaac atttgaaagt atgaattttt atccggaata
     2821 atcataactt gaatattggg aataatgccc tggttgtgag tgaattatat tttttaatcg
     2881 agaagtaggg tgattgcacg gtggcaaact ggctgaatga attctcgatt ccggcatcca
     2941 tgcttccctc aaaatcgaat gtttgtactt tgtctgacaa atactttatc gcttcgaata
     3001 cgaccagcgt ggatgctccc gaatttttat aatcagggtg aatggtagaa atcagattat
     3061 aaccggtcag gttgtcccag acaataaaga gggcagcatg aacaacactc ttcgaatcaa
     3121 tcaccgcaat tatttcaccc tgattttttg atttgcagga attgtacaaa ttcataaaaa
     3181 atgaatcgga ataaatcatt ttctgccttc tggatgtctg atatgctgta agatgaacgt
     3241 agaaatcttc tgcactcatt ttggtactta cagttaattc tttttgtgct ttacggattt
     3301 gttttttctt ggcgtagctg aatttttcaa aacatgcctc aggattgttt attcctgtaa
     3361 gttgatacgt gtatcttgtc gtttgtgaaa atttctgcca gtaaaacggc agccagttaa
     3421 cgaaattcag tggaaaattc tgattgaaat aatcgaatcc aaaatcattt agctgaacca
     3481 gcaggttgcc gattgctttt ttttcaaaat gaattttatc attatcactc aattcgttac
     3541 cataatccag ccatatcccg ttgtattgag tcagttgagg ctggatgatc attttcaacc
     3601 ccgttttttt tacaatatta catataaata caccctttat ttcatcattt tcttcgtaca
     3661 aaaatacttc ccaccaatct ttcgctttac agactgcatt catccaccac ggttgcagaa
     3721 ataaaggaac atttttgcct gaattgcaaa agttgaaata tctttcttta tttgaagagc
     3781 cttgtatcat tatggttgga ggtatcataa aaaacaaagt taccactttt ctaaaaataa
     3841 ataagtaaaa accattaatt atttaaaaaa cggacaaaat ctcaatcgat tgttttcgtt
     3901 aatctgtatc tttgtgcctc gtcgaaccaa taaaagcaga aaagtgcact gcaaaatgat
     3961 ttcaacaccc ttaaacaatc tgaaactgtc gttgcatttt tttaaaattc taaaattaca
     4021 cggtaaatga aaaagttttt tttatttatc ctgaccattt tgacaggcgg attatcacct
     4081 tcatacgctc aggataaact caccggaata ctcacaaacg aactgatgag aaattttgag
     4141 gtaatgaagc agctggacgt tccggtttat tacatcagcc tccgggtgga agactccgaa
     4201 tcgtataata ttaccggaag caacggtgca gttactcaaa acaggtatca gaaaggccgg
     4261 gcaatggcca tcaccatgcg tgtgggcagt ccggcgtttg ataatatgca ttattctggc
     4321 aatatgcaga atatcaaacg agtagccctg ccggataccg acattgaaga agaaatcaaa
     4381 ctttcgctct ggcgtgctct gcaacaggct tattccgaag cgaaagacaa acttcaatcc
     4441 aactttaccg aacaaaccac acgtcctgcc gaggaagata aatctcctga cttttccatt
     4501 gaaaaagcat ctaaatacta tgaaaagcca gttccttttt ccgatttagg atttgatgaa
     4561 aatgaattta tcaataaaac caacgaactg tctaaagtgc tgagcgacaa cagagatgtg
     4621 ttgatcaatt ttgtaaatgt aacctcaaat cttacgcgtg gatattttat tgacaccgat
     4681 ggggcatcgg ttgtccaaaa caatccttcc attcgaattt atctgggagg ttacgtcatg
     4741 gccgatgacg gaatgatgtt gcaggattac cgaacttact tcggtaagaa agttgccgac
     4801 ctgcctgatt cggaaaaaat tctttcggat gcacgggagc tgagccaaaa cctctcaaca
     4861 ctcaaaaaat cgccaaaaat cgacagctat accggtccgg tcattctgag cggcgaagtg
     4921 agcggagttt tctttcacga gtttttcgga cacagggtgg aaggtgcccg tatgaaatcg
     4981 ggtatggatg cgcagactct gaagaaaaaa attggtgaaa ctgtccttcc acaaagactt
     5041 tcggttacat ttgatcctac cagaagcaaa tatggcaact ccatactcag cggtgactac
     5101 aaatacgacg acgaaggcgt acgcggacaa aaagtggaag taatcaaaaa cggagtactc
     5161 accggattcc tgatgagtcg tatccctatc gagggattct caaaatccaa cggtcacgga
     5221 cgtggagtgt tgaatgcagg cactgaaacc cggcagtcaa acatgataat tgaaagtacg
     5281 aaaccggtaa cagctaaaga aatgcatgaa atgtttgtgg aagaattgaa aaaaagaggc
     5341 ttgaaattcg gatacagaat agaaaaagtg agtggcggat taacaatgac gagttctgcc
     5401 tcagctaatg ccttctatct caatccattg atagtttatc gagtttacgt tgatggccgt
     5461 ccggaagaaa tggtgcgtgg aatcaacatt gtaggaactc ctctgaatgc cttttcgcaa
     5521 attcttgccg aaaccagcga acatgcaatt ttcaacggca tgtgcggagc attgtccgga
     5581 agcgttccgg tatcggctgt ggctcctgca atgctgatta aggaacttga attccagaaa
     5641 acgggcgata gccagaaaat ggatccgttc attctcgaaa ggccctgata ttaacctcac
     5701 aaagatttaa tcaatgaaac aattcgtatt gaacaatata ttggctgttg tcttgttttt
     5761 gacggtagtc agtttttctg ctgcacaaac caacgatgta cttttaaatg caatgaaaac
     5821 agaagccgac cgctcaaaag ccgaattgaa acgcgatagc ctggctcgtc cgttttatat
     5881 cagcattatc tcgggcgaaa aagacattga atttacattt aacacatcgt tcggaaaagt
     5941 aaaaaaagaa ctcacagata cacgaaaatc catatatgca ggcagccagg tgcttgtagg
     6001 cgactatcat cgtacacaga atttcaccag cactaccaat cggtttactc ttccggtgga
     6061 aaacaatgaa atggctatcc gaacggccat ctggatgaat ctggatgaat tatataaaaa
     6121 cgctgccaga aattacgttg ataaaatggc ctggctcaaa caggaaaact caaaagccga
     6181 cgaactggct ctcgatgatt tcgaaaaacg caatcctaca gtgttaattc aggatattga
     6241 aaaacctgat tacgatttca gcaaagcaaa acaactttcg ttgaaaatcg gaaaacttct
     6301 caccgaaaaa gtcaatcagg aaaaactttt ggttgacgaa ctgacaactt cgtttaactt
     6361 cagtcgtgaa atagtacgat attacgatac cgaaggctcc atctacaggt atccgaaaca
     6421 ggatggcaga ttcaatatta cactcgtggg agtcactgaa gatggagaag atgtgggcgc
     6481 ttaccgaagc ttcagattga ataatccgga agaattgcca gcttttgaag atttaaagaa
     6541 agcagtaagc gacatggtga atgagtataa aataaaatat agttccgaag acctggacga
     6601 tccttatatg gggccgcttc tgatagtcga cggacaggtt gaaactgttg ccaaacaaat
     6661 agtcaacaat ctgtacactt cgccgaaaac acgtttttcg aacggtaatc attatcagaa
     6721 catgccggga gtaaggataa tttccaaaca actgtcgatg atgattaatt acggcaaaat
     6781 cgctcaacag attccgggtg tgaaacgcga cctggcacct attgatattc aggcagttgt
     6841 acctcccgat accattcttt tgataaaaga tggtattctt aaaaacatgt tgactacacg
     6901 ccagcctgta aaaaaattcc tgcattcaaa cggaaatgcc aaaaacggca attcacccga
     6961 gggagtaatt acttttaccg gaaatcaaac ctacagctac cccgaaataa agaaaatgct
     7021 cattgatgaa gcaagtctgc aggggcttga atacgcctac ataatgaatg cagatgggaa
     7081 actgcaccga aaagtgaatg tggaaacagg cgaagaaatt ccattcactg caaatttcaa
     7141 ttttggtcaa atcctgaaaa caatgcgtcg tgtacttgga gtagaagata aaatgaccgc
     7201 ctcctccgat ggcactgttc gtttcccgaa atcaatcctg ctcgaagatg ccgaactgac
     7261 agtttataaa tcaaacaaca gacttaaaaa gaattatgta accagaccgg aattatagct
     7321 taaagtattg aaaagaacac tttttgtaac caaaaatttt tgtgttgata aaaattccct
     7381 gaaaaaactt ttttaatata caaataatac ttacttttga actttatttt caatttttaa
     7441 aaacaaaata ttatggcaag atttcaggat gaatcaggca atacatcttc accgataagt
     7501 ggaggcatca tcaatataat tgcagcagga accaagatag taggtaaagt atccgttgaa
     7561 tctgatttca gactcgatgg cgaagtagaa ggtgatatct tcggaaaatc aaaagtagta
     7621 ataggcgaaa aagggaatct gaaaggttct ttaacctgtg ctaacgctga gatagtcggt
     7681 aaagtgactg gtaacatcat gacaagccat tcgcttacct tacgctcaac agccgaactg
     7741 aacggagatg tgaaaattaa atcgctcatt gtggagccga atgccatttt caacggcacc
     7801 tgttccatgc gtgaaaatgc tcaaccacag cagaatacac aaaatcaacc tgtaaaataa
     7861 tctgaaatta ataaatgatt aatcaatgac ataaaagttg ggatgcattc tcaacttttt
     7921 ttatttaaca ccctaaaaat gagattacga aattttacga tcattcaatg aaatgaaatc
     7981 attgagtggt gatttaattt gactcaaaaa aaatatattt acagatgaaa acaacagata
     8041 tcaactacaa ggcactggtg gtcagtgaaa atgaaggaac ttatgtgcag tcgattcaag
     8101 aactaaatac aaattcgtta cctgaaggcg atgtgttgat tcgggttcat tactcttcgt
     8161 tgaattataa agatgccctc tcggctaccg gaaacaaagg agtaacccga aaataccctc
     8221 acacaccggg cattgatgcc agcggcgaag ttgtggaatc tgacagccta caattcaaaa
     8281 caggcgataa agtgattgtg agcggctatg atttgggaat gaataccccc ggaggatttg
     8341 gccggtacat tcgggtgcct tcatcctggg tggtactact gccggaggaa ttatcgctta
     8401 aagaagcgat ggtaatcggc acagccggat ttacagcagg aatctccatc acccggcttt
     8461 cggaacttgt aaaaccctcc gatggaaaga ttattgtcac cggagcaacg ggaggtgttg
     8521 gctctgtggc gctcagcatt ctcagccgcc ttggttatca aacagtggca gtttcaggta
     8581 aggaaaacga attcgcgtat cttgaaagcc ttggagccgg agaaataatt ttgagaagtg
     8641 atttcctgaa tctggagaaa aagcctatac tttctgcaaa atatgccggt ggaattgatg
     8701 ctgtaggagg accaattctc gaaaatatcc tgaagtcact acttccgctt ggcgctgtta
     8761 caacttgtgg aagtgtctct tctactgaat tgaatatgag cgtttttcca tttatcttac
     8821 gtggaatttc tctgattgga atttcagccc agaattaccc catgaaactg cgcaccaccc
     8881 tgtggaacag gctggccaat gagtggaagc ccgaacatct tatgtcaatt tacaacgaaa
     8941 tcatgctgca ggaacttcct gatgccattc agcttatact caacggaaaa ctgaaaggaa
     9001 gaacactcgt taatcttaca ctttgaaaca aaaatcagct gtataaatac attttttaac
     9061 tttacagaac cgaaaaaaaa tcgcaatttt gcggtttaat atgtttcaaa aacatttttc
     9121 gaaaaagaaa tggctttagc taacgagaat tatctcaaaa ctcctgaaat atacggcttt
     9181 gatgaaattg aaaaaagaat taacgcctac aggattttga atcctaaatc tgaactgata
     9241 aatatcggat tgggtgacgt cacgctgcca ttaattccgg aagtagtcaa agctatgcat
     9301 ctggcatccg acgaattaag caaaacttct acctttaagg gatacggtcc gccgaaagga
     9361 tatgattttc ttatctccaa aattcaaaaa aactacaaaa cgaatggaat tgccattgac
     9421 aaggatacta tttttgtgaa cgatggtgcc aaatctacca ttggaaatat agggcatgtg
     9481 ctcggtcgcg acaatataat tgccatttca gatcctgtgt atcctgtgta cgaaaatgca
     9541 acaatcatga gcggacgtgc aggagatttg aacgaaaaac agcaatggag ccatatcgta
     9601 tatcttcact gctccgaaaa gaataatttc attccggact tacccaaaga aagggtggat
     9661 attatctatc tgtgcaatcc gaataaccct actggcacgg ctatgaacag aagtgaactg
     9721 aaaaaatggg tggattatgc cattgaaaat catagtatta ttgtttatga tggagtttac
     9781 agcgaattta ttacagagcc tgatattcca cgcagtatct atgaaataaa aggtgccaaa
     9841 aaggtggtcg ttgaagtgcg aacgtattct aaattagctg gatttacagg ccttcgctgc
     9901 ggatatgccg tatttccgaa tgaattaact gtgtacacgc aagaaggtga aagcattccg
     9961 cttgtaaaac tctggaaccg gcgaaattct tattacacca acggagtgtc atacattgta
    10021 cagcgtgctg cagaagctat ttattctatc gaaggagaaa ggcaaataaa agagcaaata
    10081 agttattaca tgacgaatgc tgccatgttg cgcgagcagc tcattgctct tgggcatgaa
    10141 gtttatggcg gagtaaatgc accttacgta tggatgaaaa ctcccggaaa cagttcatcg
    10201 tgggattatt tcaacgaatt gctttatttg aaaaagataa ttgtaacgcc cggagtactt
    10261 tttggaaaaa acggagaagg atatatacgc ttcagtgggt ttgccaaccg cgaaatgatg
    10321 aaactggcat tagaaaaatt taaaacttaa acgatttttt tttaattaaa aattgacaac
    10381 aatatgtgtg gaatagttgg atatattggt aacagacagg cttatcctat tctgataaaa
    10441 ggacttcacc ggcttgagta ccggggttat gacagcgccg gaatagctct cattaatcac
    10501 aacaacctta aagtatataa agcaaaagga aaagtatctg aattagagca ttttgcagaa
    10561 caaaaagata taagtggcac tatcggaata gctcacacac gctgggctac tcatggtgaa
    10621 ccaaatcaaa aaaatgctca ccctcactat tcgcagtccg aagaaattgc catcattcac
    10681 aacggaatta tcgaaaatta tgccgttttg aaagaagggc tgatgaaaga aggatataca
    10741 tttcagagtc agactgatac ggaagtgttg gtgcagctga ttgaatacat gaagaaaacg
    10801 aataactccg atctgccaac agctgtacaa cttgcattga atcaggtggt tggtgcttat
    10861 gcaattgcag ttctggaaaa aaataatccg gatttaatcg tagcagcccg caaaggcagt
    10921 ccactggtta tcggtatagg tgaagatgaa tatttcatcg cctctgatgc cacacccatc
    10981 gtagaataca ccaataaggt tacttacatt ggtgaagaag aaatagttac gataatgcgc
    11041 aacgagccgc tcaagatcaa aactatcggg aatatcgaaa aaacacctca catccagaaa
    11101 ctcgaaatga cactgagtca gctggaaaaa ggcggttatc ctcacttcat gctcaaggaa
    11161 atctttgaac aacccaaaac actcaatgac tgtatgcgtg gaagagtgaa tgtggactct
    11221 gataacatta cgttagccgg atttatcgat aacagggaca aatttctgaa tgccaaacga
    11281 ataatcatta ctgcctgtgg aacatcctgg catgctgctc aaattggaat gtatgccatc
    11341 gaagaatttg cgcgcattcc ggtagaagtg gaatattcct ccgaattcag ataccggaat
    11401 ccggtaatca acaaagatga tattgtaatt gccatctccc aatcgggcga aacagccgat
    11461 acactcgccg ctgtagaact tgccaaaaaa cacggggcat tcatctttgg gatatgcaat
    11521 gtagttggtt catctattcc tcgaaacact cattcagggt gctacaccca cgtcggacct
    11581 gaaataggag tggcatctac caaagctttc actgctcagg ttaccgtgtt gaccatgctg
    11641 gccattctga tagggaagga aaaagaaact ctctcaaacg aagagtacct gaaattaatt
    11701 aaagaactaa gtcttattcc tgataaaatc agtgaagtac tccagaaaaa cagtcagatt
    11761 gccgaaatgt ctaagacatt tacctatgcg agtaattgta tttacctggg tcgtggttac
    11821 aattttccgg ttgcactgga aggcgctctg aaactgaaag aaatatctta tatacatgct
    11881 gagggttatc cggctgccga aatgaaacac ggaccgatag cgctaattag caatgaaatg
    11941 ccggttatag ccattgccac tcagtcagat acatacgaaa aggtaatcag caacatacag
    12001 gagatcaaag cccgaaaagg cagaattatt tccatagtca ccgagggcga cgaagttgtc
    12061 agaaaactct catcgtacag catcgaagtc cctaaaacag aaacatgcct tacaccttta
    12121 ctttcggtaa ttcctctgca gctgctggct taccacattg ctgttgtaaa aggatgtgat
    12181 gtggaccaac ccagaaacct ggctaaatca gttactgtag aataaaagat atatgtgtgg
    12241 aatagcaatg ataaggcttc tgaagcctct tgattattat cagaaaaagt acgattcctg
    12301 gcagtacgga ctcaacaaga tgtatctgct gatggagaaa cagcataacc gggggcagga
    12361 gggagccgga atagccactc tgaaactcga tatcactccg ggagaagaat acatctggcg
    12421 cgaacgcgag gaaggaaaaa atgccattca gcaaatattt gccaacatca acgaaaaaat
    12481 tgaaaaaatc aacggacttg ttgacgatcc ggcattcgca aaagaaaacc tcccttttgc
    12541 cggggaactt tacctgggac acttacgtta cagtacaacc ggaaaaagcg gtatctcata
    12601 tgtacatcct tttcttcgtc gacacaactg gaaagcccgc acactggtaa tggccgggaa
    12661 ttttaatctg acaaacgtag atgagatttt cgaaaatctg actgagcagg gacaacatcc
    12721 gcgtgataaa gcagatactt ttctgatgct cgaatcgctt ggaaataaac ttgataacga
    12781 agtacaggca gaatacgagc aaatcagaaa aaggtattca aacgataaaa ttatcaccca
    12841 aaaaattgag gaacaactcg atatcccgtc gttcatacgt aaatctgaga aaaaatggga
    12901 cggtggatac gttatttgcg gtatgcttgg acatggagat tcgttcgtat ttcgcgatcc
    12961 ggatggaatc cggactgctt tttactacca ggatgatgaa atcgttgtag ttgcctcaga
    13021 aagagctccc ctgcaaacag tgatgaatgt caaaaccgaa gatatcaggg aactgcaacc
    13081 tggccaaatc atgatcatta aaaagaatgg aaacgtcctt ttcgatacga ttagaattcc
    13141 ctctgccgaa attaaagcct gttcgttcga aagaatatac ttctcaagag gcagtgatgt
    13201 ggaaatttac aacgaaagga aaaaattagg agcaactctt gccgaaccaa ttcttaaaag
    13261 tatagattat gaccttgaga attcggtctt ttctttcata ccaaataccg ctgaagttgc
    13321 tttttacggc atgacacaag gtatcagaag tataaccgga aaagatcctc atgtagagaa
    13381 agtattgatt aaagacatta agcttcgcac cttcatatcg cagaataaag aacgaaacga
    13441 cctggcgaaa catgtttacg acgttacctg gaattcaatc cgacgcggaa aaaaagacaa
    13501 tctaatagtc attgatgatt ctattgtgag aggtactaca ctgaaacaga gtattttatc
    13561 aataattaac cggcttgaac ccaaaaaaat tgttgtagtt tcgtcatcac cacaggtccg
    13621 gtatccggat tattacggaa tcgatatgtc aaaaatgagc gaattttgtg cattcagagc
    13681 tgctatagcc ctgcttaaag aaagcggcag gcaacaactg attgacgaag tttatcagga
    13741 atgtaaatca caggaaaacg aagtaaaaga gaaaatagta aattatgtaa caaaaatcta
    13801 cgaaccgttt actcaggaag aaatttcaca aaaaatagca gaaatcctga ctcccgcaga
    13861 tgtggaatgt cctgtggatc ttgtttttca aactgttgaa ggtctgcacg cagcttgtcc
    13921 gaaccataca ggcgactggt attttacagg taattatccc acatcgggtg gaaaaagact
    13981 tgttaatcac gcatttatta attattacga aggaaaagaa aaacaatgat agaaataatt
    14041 cctgccattg atctaattga tggtaaatgt gtcagactca ctcagggcga ctactcacaa
    14101 aagaaaattt acaacgaaaa cccaatcgaa gttgccaaaa tgtttcagga tgcgggcatt
    14161 tccaggttac acctggttga tctggatggt gctaaggcac aacacattgt caattatata
    14221 gtacttgaga gaattacttc gaaaacagac ttaatcgtgg attttggggg tggtctcaaa
    14281 tcggatgatg atttgcgtat tgcatttgaa tgtggagcct ccatggtgac cggtggaagt
    14341 attgccgtta aaaatcctga aatatttact ggatggatag aaaagtacgg ttcagaaaaa
    14401 atcatgctgg gagctgatgt gaagaatatg aaaatagcag taagcggatg gatggaagat
    14461 actgatattg agcttattcc attccttgaa acctatcaga aacgaggaat ttcaaaaata
    14521 atctgtaccg atataagcaa ggacgggatg ctcgaagggc catcaataga gttgtataga
    14581 gacatcattc gcaaactgcc tgacgttttt ttgatagcaa gcgggggagt aagttctata
    14641 gctgatattg aaatactcca cgattgcggt gtaccggcag tcatcttcgg aaaagccata
    14701 tacgaaggaa aaatcaaatt aaaggattta tcacgatttt taatgtaatt gaataaaaga
    14761 ttttgcaaaa aaaatactta tatttgtact gaatttggtc tgatttttta tcagatcatt
    14821 tttttctccg aaataaatca attgaacatc agataagtta atacattaat ttacacatta
    14881 tgcttaaaga aatattatcc attaccggca aaccgggtct ttacaaactg gtttctcagg
    14941 gtaaaaacat gttgatagtt gaatcgctca tcgatggtaa acgaattcct acatatgcca
    15001 aagacaaagc tgtttcgctt ggcgacattg ccatttttac tgaaaccgaa gaagttccgc
    15061 ttggcaaagt actcgaaaaa gttaaatcga aagaaaatgg tctgaaagct tccatcgatt
    15121 cgaaatcgga aaatgatgaa ctgagaaaat acatggaaga ggttttgccc gaatatgata
    15181 aagacaaagt ttatccaagt gatatccgaa agcttatcaa ctggtacaac ctgctgatag
    15241 agagtaatat cactgaattt gtggaaaaag aagaggaaac tgaagagcat ccggaaaaag
    15301 aattggaagt gaaggagaaa ggtaaaaaag ataccaaacc tgcagcagcc aaaaaagaac
    15361 caaaagttca ggtaaaacaa ccgaaaatag ctcagaaatc catgaccaac cgtaaaatgg
    15421 gtggataaat cagcaatgaa gtaccggaaa aaatgatttt tctgaaaagg gaaaaggtta
    15481 agttttc
//
LOCUS       DAORKP010000037        15385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_284_length_15385_cov_8.933594, whole genome shotgun sequence.
ACCESSION   DAORKP010000037 DAORKP010000000
VERSION     DAORKP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15385
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_15385_cov_8.933594"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3195
                     /locus_tag="PLN75_03510"
     CDS             <1..3195
                     /locus_tag="PLN75_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRG03219.1"
                     /translation="KVHTTYLWHMDQPVYWADKSKEKPDSKQFAEESHRLKMSGQNMY
                     PGSAVAHPTNNLEEIFGKADRVNAYQYTPKTAIQSISSYPDAGAQLSISAGLLENIQS
                     LGVKNQWGYSAGWMNAYKDAYGWKTRDGFPKLDIVNFTYDHALSPLVSERTLKKQILA
                     HKYVTNKYYGSSSKGYWPAEGAFSERIIKALAECGIEWSIIANSHLARTLSDYVHPYN
                     INGNIEAPNKADRVETEGINWFDATIDGRGSRLAVPYSYQAHKAQYVDPNNGQIYRID
                     VVPMCNYLSYVDGYSGANAGEVAQKIEPFSTTAHPSIVLLAHDGDNAWGGGSSYYNEA
                     VSGFTSGAAAAGYKPTTIQQFLKDNPVPATDVVHVEDGAWVNAENDWGHPQFINWIWP
                     LFSTSNYRFNPNGWTEDARNWAVITATENYVTMAEDLEGGILRINYIADGGTAATNAE
                     KAWHFYFGGLNSGFMYYGKAEDMEVKPSMTGNIAIEYAQKVIDAKPGTDNTGPSVFIP
                     QRFPYNPGETGFSPYTGYKKVNYSSDFHVWTFAYDVSGLTSVTLKYRVDLDGKNPISS
                     TQNDTYVGGSEVEPWQNIEMTRQPMAADPTDPELNFFIQPKAKADLCYAEIAGLKDKL
                     VDYYVEATDTKGNIFKTPIQHVYIGTGEGQLPTDGNVTWLPASPTSLETITITCKNAT
                     AASKLHWGVNGWNAPDGVYRPEGTVAVSAKAVETPFRQAEGKWQVVLGPFNNPAQAVT
                     AVNFVIKHESGWDNNGGSDYLITISAAQTNNPTGTNITKTLDQNSSYSFSPGDFNFAS
                     STGNTFKGIKILSLPASGTLTTNGANVSVNQLVMNVSQLTYTAATSSSSFTFRIVDSA
                     NLESDAVYTASFPVADPAQQAITVQFKKPDDWGTTPVYLWAWTGSGNLFTSWPGVAMT
                     AQQNNWYAYTFDKSITSVNVIFSKNGSPQSVDIMGVSQSTCYETDNPAGKFTVRETTC
                     GTTGMENKLIAQTISIYPQPVKSEFYLNLPNTGYTGNYILNIINLQGKMIQETHFTGQ
                     TAHIQLLTESSGVYLIKIRNVNGSEIYTSKIVKM"
     assembly_gap    3367..3466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3468..3541)
                     /locus_tag="PLN75_03515"
     tRNA            complement(3468..3541)
                     /locus_tag="PLN75_03515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Arg,seq:ccg)
     gene            complement(4127..4846)
                     /locus_tag="PLN75_03520"
     CDS             complement(4127..4846)
                     /locus_tag="PLN75_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRG03220.1"
                     /translation="MYDYPAATTVGVNYLPYILFGAVALLSWLVQINFQSKFRKYSQI
                     LMSHGMTGRQVAEKMLRDNGITDVQVISTPGQLTDHYNPQNKTVNLSEAVYASNSVAA
                     AAVAAHECGHAVQHATAYGPLQMRSRLVPVVSIASKWLQWILLGGIVLLNIFPGLLLF
                     GIILFAMTTLFSIITLPVEINASQRAIAWLSSTGVTNYETDDAAKGALKAAAYTYIIA
                     ALGSLATLFYYLMIFLGRSRD"
     gene            complement(4974..6248)
                     /locus_tag="PLN75_03525"
     CDS             complement(4974..6248)
                     /locus_tag="PLN75_03525"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRG03221.1"
                     /translation="MKKVDVLLGLQWGDEGKGKVVDVLTPGYDVVARFQGGPNAGHTL
                     EFEGQKFVLRSIPSGIFQGDKVNVIGNGVVLNPALFKEEVDALTKSGHPLTERLKISK
                     KAHLILPTHCLLDKAYESAKGTAKIGTTGKGIGPAYTDKISRNGLRVGDILNNFEEKY
                     KKATERHLEILKSYDLDFDLSGLEKDWFEGVECLKQFEFIDSEHFINDAMKNGKKVLA
                     EGAQGTMLDVDFGSYPFVTSSNTVSAGSCIGLGVAPGNIGEVFGIFKAYCTRVGSGPF
                     PTELFDEEGDSMRDKGHEYGSVTGRPRRCGWIDLVALKYAIMINGVTQLIMMKGDVLE
                     EFDTIKACVAYKINGKETESLPFDLSEVTEPVYAELPGWQADLTKLQSENEFPEELNA
                     YLDFLEDELGVPVKIVSVGPDRDQTIFRHQEN"
     gene            complement(6382..6840)
                     /locus_tag="PLN75_03530"
     CDS             complement(6382..6840)
                     /locus_tag="PLN75_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRG03222.1"
                     /translation="MKEDYSLDTVKKIFTQFLAERKHRKTPERYAILEHIFSHEGHFN
                     AELLYNAMQKEYRVSLATVYNTLELLLDSRLIVKHQFGEQVAQYEKTFGNTMHNHMVC
                     TKCGKVKEFSDKKIRAAIQSKRFVHFESTHYSLYLYGVCSKCKAELNKKQ"
     gene            complement(6909..7592)
                     /locus_tag="PLN75_03535"
     CDS             complement(6909..7592)
                     /locus_tag="PLN75_03535"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRG03223.1"
                     /translation="MKFFIQNNELDKSVQEIRRKIRTLMNGVVADSMKDKGIVYKKNF
                     GVDLPHLRDLARKYPPGHDLAQRLWALQIRETMILATLLEPADTFAESMAGEWMQSVN
                     NIELVEQICMNLFSKLSYGSQLAIRLIHVDQKWSRITGFTLSARLWKDLDNEQVRELV
                     HLAIENADTEDFYLYRAVSLSLCRLCRRDKATAEHIQNQTETFVNSDKLSHRYIAGEI
                     SNEISFLNF"
     gene            7674..7749
                     /locus_tag="PLN75_03540"
     tRNA            7674..7749
                     /locus_tag="PLN75_03540"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7709..7711,aa:His,seq:gtg)
     gene            7918..9231
                     /locus_tag="PLN75_03545"
     CDS             7918..9231
                     /locus_tag="PLN75_03545"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRG03224.1"
                     /translation="MKVLKFGGTSVGSAARMKDVAKLILNGEQKIVVLSAMSGTTNSL
                     VEIADYFYKNNTSGALERINMLEHTYMDVISELYAEEKYLNKAQEIIHTHFEYLRSFS
                     KSVFTVFEEKAILAQGELLSTNMFQLYLEEKGVNSVLLPALEYMRIDKNSEPDNAFIA
                     ENLKKLLSENKGKDIYITQGYICRNFYGEIDNLQRGGSDYSASLIGAAIKADEIQIWT
                     DIDGMHNNDPRYVEGTKPVPMLHFEEAAELAYFGAKILHPTCILPAKLHNIPVRLLNT
                     LDPSAPGTIISNKRKRGIIEAVAAKDSITAIKIKSGHMLLAYGFLRKVFEIFESNQTP
                     IDMITTSEVGVSVTIDNTRRLEEILNDLKKFGTVTVDKDMVIICIVGDMEYELAGFQA
                     KVINALKDIPLRMVSYGGSNFNISVLIKAEDKKKALNLLSEALFN"
     gene            9300..10457
                     /gene="lysA"
                     /locus_tag="PLN75_03550"
     CDS             9300..10457
                     /gene="lysA"
                     /locus_tag="PLN75_03550"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRG03225.1"
                     /translation="MKAHYPTDKFQQISTPFYYYDLDVLRSTLSEIKMLDLPENYMVH
                     YAMKANVNPSILEVIKNAGFGADCVSGGEIQAAIDAGFPASKIVFAGVGKSDWEINLA
                     LEHDIFCFNAESVPELEVINELAASKNKKARVALRINPDVSAHTHKYITTGLSENKFG
                     INMEDLDHVISLLPKLTHLEFTGIHFHIGSQITDMTAFHNLCVRIEEIQDIFNTKNIE
                     ITHINVGGGLGVNYEHPNHFPVPEFETYFKTFRDHLGLKPGQTLHFELGRSIVAQCGS
                     LISRVLYVKEGSTRKFVILDAGFTDLIRPALYQAYHRIENLSSEKPTCLYDVVGPICE
                     SSDVFAKDFMLNETKRGDLIAIRSAGAYGEIMASQYNLRSLPQAYTSDELK"
     gene            10488..11660
                     /locus_tag="PLN75_03555"
     CDS             10488..11660
                     /locus_tag="PLN75_03555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03226.1"
                     /translation="MDFFGIIRFSVVEISLLAVLLTAFIVQLYFYIRYFMGVIRHNKR
                     VSKGIEQFRNDQPPVSVIICARDEEENLRKFLPFVLEQDYPDYEVIVINDGSTDHTDD
                     YLSLMVKQYEKLRTTFVPGGATNVSTKKLGLTLGIKAAKNDLLLFTDADCMPEGKNWL
                     EMMVRNFTPETEFVLGYGGYLPKKGFLNKLIKYDTLFIALQYLGMAASHRPYMGVGRN
                     LAYRKDTFFNLKGFATTLNLMSGDDDLMVNRGATTENTRIEVSSESVTWSEPKTTFRN
                     WLIQKERHLSVSDKYNTPSKLRLTVEPVSRGIFYLAFVTAVVVSGLSFNWVAAAFAGL
                     LFIVRFTLQLLIINKSAKILGERRFYFTIPLFDSILPLITLSILTFGKKKRNLKWK"
     gene            complement(11853..12878)
                     /gene="tdh"
                     /locus_tag="PLN75_03560"
     CDS             complement(11853..12878)
                     /gene="tdh"
                     /locus_tag="PLN75_03560"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRG03227.1"
                     /translation="MKALVKAKAEKGIWMEDIPVPEIGINDVLIKIKKTSICGTDLHI
                     YKWDDWAQNTIKVPMTIGHEYVGVVVEKGRGVKNVKIGDRVTGEGHIACGHCRNCRRG
                     KLHVCENTIGVGVNRDGAFAEYLSLPASNVVHLDDRISDETASIMDPFGNATHTALSF
                     PLIGEDVLITGAGLIGTMATGICRFAGARYVVVSDFSDYRLEIAKKMGATLTVNPSKG
                     ETIQGAMKELHMRGFDVGLEMSGAPTAFRDMIDNMYNGSKISLLGILPNTTTVDWNKI
                     IFKALTLKGIYGREMWETWYKMEQMLISGFDLNPVITHRFGIDEFQQGFDIMESGQCG
                     KVILSWE"
     gene            complement(12935..14125)
                     /gene="kbl"
                     /locus_tag="PLN75_03565"
     CDS             complement(12935..14125)
                     /gene="kbl"
                     /locus_tag="PLN75_03565"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRG03228.1"
                     /translation="MYQNFKSFLQSELQGIENAGLYKNERIIVSPQGAAIRVSDGKEV
                     LNFCANNYLGLSNNQQLKDAAKSAIDTHGYGMSSVRFICGTQDLHKQLETSIARFFGT
                     EDTILYAACFDANGGVFEPLLSAEDAIISDALNHASIIDGVRLCKAQRYRYANADMAD
                     LEEQLKKAQAQRFRLIVTDGVFSMDGNVAPLDKIYELAEKYNAMVMVDESHSAGVVGQ
                     TGRGVTERYNLKGKIEIITGTLGKAFGGAIGGFTTGKKEIIDLLRQRSRPYLFSNSIP
                     PMVAAAGIRMFEMMDETNDLQDKLHANTEYFVAKMKAAGFDIKPTESAICAVMLYDAK
                     LSQEVANRLLDEGIYVIGFYYPVVPKGEARIRVQISAAHEKTHLDTCIDAFTKVGKEL
                     GVIG"
     gene            complement(14294..14911)
                     /locus_tag="PLN75_03570"
     CDS             complement(14294..14911)
                     /locus_tag="PLN75_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRG03229.1"
                     /translation="MRRHNYIAWVLLGITVLLSVLLYRAKYIPQKTAYNSSAIMEKIS
                     YVKDLSLVKYNYSGVISYKDYMKFMNLQVPLTEKSFLIRYNGYVKAGIDMSKATVTVN
                     GKSVKIGLPKPQIQETVIDEKSLQVYDESMNILNPIKVTDYQNAMVAEQHKIKQDALA
                     KGILTESSDQAHKFITSLLEELGFEKIEIDEISSIILPGNEIKKK"
BASE COUNT         4405 a         3377 c         3199 g         4304 t
ORIGIN      
        1 aaagtccaca ccacctacct ttggcacatg gaccagcctg tttactgggc cgataaaagc
       61 aaggaaaaac ccgatagcaa acagtttgca gaggaatcgc accggctgaa aatgagtgga
      121 caaaacatgt atccgggtag tgctgttgcc caccccacaa ataatctgga ggagatattc
      181 ggtaaagccg accgcgtgaa tgcctatcaa tacactccca aaacagctat tcagagcatt
      241 tcatcgtatc ctgatgccgg tgcgcagctg agtatttctg cagggctgct tgagaacatt
      301 caatcgctgg gcgtaaagaa tcagtggggc tattcggccg gttggatgaa tgcatataaa
      361 gatgcctatg gatggaaaac acgggatgga tttccaaaat tagacattgt aaatttcacg
      421 tacgatcacg ctctctcacc gcttgtaagc gaaagaacgc tgaaaaaaca gattctggct
      481 cataaatatg tgaccaacaa atactacgga agttcttcaa aaggttactg gccggctgaa
      541 ggcgctttca gcgaacgaat tattaaagca ctggctgaat gtggtattga atggtcgata
      601 attgccaaca gccacctggc gcgtacgctc tccgactatg ttcatcccta caatattaat
      661 ggaaacattg aagctcccaa caaggctgac cgggtagaaa cagagggtat caactggttt
      721 gacgccacta ttgacgggcg cggaagccgg ctggcagttc cctacagcta tcaggctcat
      781 aaggcgcagt atgtcgatcc gaacaatggt cagatttaca ggattgatgt agttccgatg
      841 tgtaattacc tgagttatgt agacggttac agcggtgcaa atgccggcga agtagctcaa
      901 aaaatcgaac cgttcagcac cacggctcac ccgagtatcg tgctgcttgc tcatgacgga
      961 gataatgcct ggggaggcgg aagcagctat tacaacgagg ctgtatcggg ttttacgagc
     1021 ggtgcagctg ctgccggata caagccaacc accatccagc agtttctgaa agataatcct
     1081 gtccctgcta ccgatgttgt acacgtagaa gacggtgcct gggtaaatgc cgaaaacgac
     1141 tggggtcatc cacagtttat caactggatt tggccgcttt tctcaacatc caactaccgt
     1201 tttaatccaa acggttggac agaagatgcc cgcaactggg cggtgattac cgctaccgaa
     1261 aattatgtca ccatggccga agacctggaa gggggaattt tacgcataaa ctatattgct
     1321 gatggcggta cagccgcaac caatgccgaa aaagcctggc acttctattt tggaggactg
     1381 aattccggat tcatgtatta cggaaaagcg gaagacatgg aggtaaagcc ttccatgacc
     1441 ggaaatattg ccattgaata tgcccaaaaa gttattgatg caaaaccggg aaccgacaac
     1501 accggaccat ccgtttttat tccgcaacgt ttcccctata atccgggcga aacaggattt
     1561 agtccataca caggttacaa aaaagtgaat tactcaagcg acttccatgt atggacattc
     1621 gcgtatgatg tgagcggatt aacttccgta actttgaaat accgagtgga tttagatggt
     1681 aaaaatccga tatcgagtac tcaaaatgac acctatgtgg gcggttctga agtagaaccc
     1741 tggcaaaata tagaaatgac gcgtcagccg atggcagctg atccgacaga tccggaactg
     1801 aattttttca ttcagccaaa ggcaaaagcc gatctttgct acgccgagat tgcaggactg
     1861 aaagacaaac tggtagatta ttatgtggaa gccaccgata ccaaaggaaa tatattcaaa
     1921 acacctattc aacatgtgta tattggaaca ggagaaggac agctgcctac cgacggcaat
     1981 gtgacctggt tacctgcatc acccacatca ctcgaaacca tcacaatcac ctgcaaaaat
     2041 gccacggcag catcaaaact gcactgggga gtaaacggat ggaatgcacc ggatggagtt
     2101 taccggcccg aaggaaccgt ggccgtatca gcaaaagcag tggaaacacc tttcaggcaa
     2161 gcagaaggga aatggcaggt agttctaggt ccattcaaca atccggctca ggcagtaaca
     2221 gcggtcaatt ttgtaataaa acacgaatcc ggctgggata ataacggtgg aagcgactac
     2281 ctgataacca tttctgctgc gcaaaccaac aatccaacag gtacaaatat cacaaaaacg
     2341 ctggatcaaa acagttctta cagtttttca cccggtgatt ttaacttcgc aagttccaca
     2401 ggaaatacgt tcaaaggaat aaaaattctg agtcttccgg cttcgggaac tttgacgaca
     2461 aacggagcga atgtatctgt caatcaactc gtcatgaatg tttcacaact gacatataca
     2521 gccgcaacga gttcatcatc tttcactttc agaatagtgg acagcgccaa tctggaaagc
     2581 gatgcagtgt acacggcaag cttcccggtg gctgatccgg ctcagcaggc aatcaccgtg
     2641 cagttcaaaa aaccggacga ctggggcacc acacctgttt atctgtgggc ttggaccggc
     2701 tcaggtaatc tattcacatc ctggcccgga gtagccatga cagcacaaca aaacaactgg
     2761 tacgcttata ctttcgacaa gtcgattacc tcagtaaatg taattttcag taaaaacggt
     2821 tctccacaat ccgttgacat aatgggagta agtcaatcga cctgctatga gacagacaat
     2881 ccggccggta aatttaccgt cagagaaacg acctgtggca ccacagggat ggagaataaa
     2941 ctgattgcac aaaccatcag tatatatcca caacccgtaa aatctgagtt ttatctgaac
     3001 ttgccaaata ccggttatac aggaaattat atactcaata tcataaattt acagggaaaa
     3061 atgattcagg aaacacattt tacaggacaa acagcccaca ttcaacttct gactgaatca
     3121 tcgggtgtat atctgatcaa aatcaggaac gttaacggaa gtgagattta cacatcaaaa
     3181 atcgtaaaaa tgtagattaa aacgagaata taattgaact ctaaaaacac aaaaaaagct
     3241 tacaaaatgt atgtaaaata cgtttgtaag ctttttgatt atttgattta caatgtttta
     3301 taaatcaaaa tgtgaccccg gcgggattct aacccacgac cttcagaacc ggaatctgac
     3361 gttctannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtga ccccggcggg
     3481 attctaaccc acgaccttca gaaccggaat ctgacgttct atacagctga actacgaggc
     3541 caaattgaat cagttttttt acttattcgg ctacaaagat acaatatttt ttttattcca
     3601 taattgttta gaaaagttac aaccggattt aaaaccaaaa tttctattca tcattatgaa
     3661 tcatcctttt aatgaaattc cgaccttgcc cgctttttaa aaatttttct ctggctctgg
     3721 cttcactcct ggttgaatac tcttcataat acacaagttc ccatggaaca aatactttcg
     3781 ttgaaaaggt acgtcctgca ttgtgttctt ttattctatt tgtaaaatta gtagtcatcc
     3841 ctttgtaatt tctgccatcc ttcaaactct ttctatatac acaaagtgtt tcataataaa
     3901 actttctata gagaagaaaa aaagactatc tcgaaatagt cctttaaagt gtatgaaatg
     3961 atgatgtgtg tttttcagac cttcagaacc ggaatctgac gttcccagct tgcagcaggc
     4021 aagtatacag ctgaactacg aggcccgata actctcaacc cctggctgaa aagtggtgca
     4081 aggatataac aaataattta aaactgcggc agtggcacgc aatttattaa tctctgctgc
     4141 gtccgaggaa aatcatcaga tagtaaaaca gtgtggccag tgaaccgagc gcagcaatga
     4201 tatacgtgta tgcagctgcc ttcaacgctc ctttggctgc atcgtcggtt tcataattgg
     4261 tcactccggt actgctcagc caggcaatgg cgcgttgcga agcattgatt tcaaccggca
     4321 gtgtaatgat gctgaacaaa gttgtcattg caaacaaaat gatgccgaaa agcaacaacc
     4381 ccggaaaaat attgagaagc acaatacctc ccagaagaat ccactgaagc catttggagg
     4441 caatgctcac taccggtaca agcctggagc gcatctgcaa aggtccgtat gctgtagcat
     4501 gctgcactgc gtgtccgcac tcgtgagcag ctactgcggc agctgccaca ctgttactgg
     4561 catacaccgc ttcgctcaga ttcacggttt tgttctgtgg attgtagtgg tcggtaagtt
     4621 gtccgggtgt ggaaattacc tgtacatcgg ttattccgtt atcacgaagc attttctcgg
     4681 ccacttgtct gccggtcatg ccgtgcgaca tcagaatctg ggaatatttt ctgaatttcg
     4741 actggaaatt gatttgaacc agccagctca ggagtgcaac agcaccaaac aaaatataag
     4801 gtaaataatt aacgccaact gtagtggctg caggataatc gtacataatt cagtatattt
     4861 aaaaagttac taatttgaat ctatcaaaat gtaagccaat tgaaatgact ttgtatctta
     4921 aataaacaaa aaaatgctca ctttgttttg atgagcattt attcataatt tttttaattt
     4981 tcctgatgcc tgaatatggt ttgatctctg tcaggaccta ccgaaacaat ttttaccgga
     5041 acacctaatt catcttccag aaagtcaaga taggcattca attcttcggg aaattcattt
     5101 tcactctgca attttgtcag atcggcctgc catcctggca gttcggcata caccggctcg
     5161 gtcacttcgc tcaggtcgaa aggaagcgat tcggtttctt tgccgttaat tttataagcc
     5221 acacaggctt tgattgtatc aaattcttcg agcacatcgc ctttcatcat gataagctgt
     5281 gttaccccgt tgatcatgat ggcgtattta agcgctacaa ggtcaatcca tccgcaccgg
     5341 cgcggacgtc cggttaccga accgtattca tgacctttat cgcgcatgct gtcaccttct
     5401 tcatcaaaaa gttcggtagg aaacgggccg cttcccacac gcgtacaata ggccttgaaa
     5461 atgccaaaaa cttcacctat gttgcccggc gctactccca gaccaatgca ggaaccggca
     5521 gaaacagtat tggaagatgt tacaaaagga tacgagccaa aatccacatc gagcatggtg
     5581 ccctgagcgc cttcagcaag cactttcttc ccgtttttca ttgcatcatt tataaaatgt
     5641 tcgctgtcaa tgaattcaaa ttgcttcagg cattccactc cttcaaacca atctttctcc
     5701 aggccgctca aatcaaagtc caggtcatag ctcttaagta tttccaggtg tcgttcagta
     5761 gcttttttgt atttttcttc aaaattattc agtatatcgc ccaccctgag tccgttacgg
     5821 cttattttat ctgtataagc cggaccgata cccttgccgg tagttcctat ttttgccgtt
     5881 cctttggccg attcataggc tttatcgagc aggcagtgag tgggtagtat caggtgtgct
     5941 tttttggaaa ttttcaaccg ttcggtaagc ggatgtcccg attttgtgag tgcatccacc
     6001 tcttctttga aaagcgcagg attgagtacc acaccgtttc cgattacatt gaccttgtcg
     6061 ccctgaaaaa ttcccgatgg aatggagcga agcacgaatt tctgcccttc aaattcgagg
     6121 gtgtgacctg cgttgggtcc tccctgaaaa cgtgccacaa catcataacc gggtgtaagc
     6181 acatccacca cttttccttt tccttcgtcg ccccactgaa ggcctaataa tacatcaacc
     6241 tttttcatat ttgttattct ttcaattttt ttattttttc cccggataaa aaaacacctt
     6301 gtgaaatagc cagttcacac agtttaaatt tcttaaagat ggattaaact tataaaaata
     6361 attccacgca gagttttcta ttcactgttt tttgttgagt tcggccttgc acttgctgca
     6421 tacgccgtat aaatacaacg agtagtgtgt ggattcaaaa tggacaaagc gcttgctttg
     6481 tatggctgcg cggattttct tgtccgaaaa ttctttcacc tttccacatt tggtgcacac
     6541 catgtgattg tgcattgtgt ttccgaatgt tttttcatat tgagctacct gctctccaaa
     6601 ctgatgttta acaatgagcc gactatccag aagcagttca agtgtattgt aaacagtcgc
     6661 cagactcact ctgtattctt tttgcatggc gttatagagc agctcggcat tgaaatgtcc
     6721 ctcgtgagag aaaatatgtt cgagtatcgc ataccgttcg ggagtcttgc gatgctttct
     6781 ttctgccaga aattgagtaa aaattttttt taccgtatcc agggagtagt cttccttcat
     6841 taaaaccagt ttaaatgttt tttttgaaaa taacgagcaa aaatacgatt tttaaatgac
     6901 actttgtatt aaaaattcaa aaaagaaatt tcatttgata tttcaccggc aatgtatcgg
     6961 tgagatagtt tatcggaatt tacaaatgtt tctgtctgat tctgaatatg ctcagcagtc
     7021 gctttatcgc gccggcaaag tctgcaaagt gacaatgaaa cagccctgta gaggtaaaaa
     7081 tcttcggtat cggcgttttc gatggccaga tgcaccagtt cccggacctg ttcgttgtcc
     7141 aggtctttcc aaagtcgggc agacagcgta aaaccggtta tacgactcca tttttgatca
     7201 acatgtatca gccgaatggc caattgactg ccgtatgaca attttgaaaa caaattcatg
     7261 cagatttgtt ccaccagctc gatattattc acagattgca tccattcgcc cgccatggat
     7321 tcggcgaatg tgtctgcggg ctcgagtaat gttgccagaa tcattgtctc acgtatctgc
     7381 agtgcccaga gtcgttgcgc aaggtcatga ccgggagggt attttcgggc cagatccctg
     7441 aggtgaggca gatctacacc aaaattcttt ttatacacta ttcccttgtc tttcatggaa
     7501 tctgccacca cgccgttcat cagtgtacgt atttttcttc tgatttcctg tacggatttg
     7561 tcgagttcgt tgttttgaat aaaaaatttc attgttgcgt ttaattgcgt tttttctaaa
     7621 aataaatttg cacaatccca aaatcagcat tatctttgca cccacaaaaa catggtggtt
     7681 gtagctcagt tggttagagc gtcggattgt ggttccgaag gtcgcgggtt cgagccccgt
     7741 tttccaccct caggagaaaa ggaaattcag caatgagttt cctttttttt atcgggatta
     7801 ccggctgcaa aattacgcca aatattgaaa ctgacaaatc aaccatattt gtaaaaaaag
     7861 tgttactttt gcacctgaat ttattttacc caaaaccatt tatttgttat cagcaatatg
     7921 aaagtactaa aatttggcgg aacttcggta ggttcggctg cacggatgaa agatgtggcc
     7981 aaactgattt tgaacggcga gcaaaagatt gttgtattat cggccatgtc gggcactacg
     8041 aacagcctgg tggaaatagc cgattatttc tacaaaaaca atacttcggg tgcgcttgag
     8101 agaattaaca tgctggaaca cacgtacatg gatgtaatca gcgaactcta tgccgaagaa
     8161 aaatacctga ataaagctca ggaaataatt catacgcatt tcgaatacct gcgttcgttt
     8221 tcaaaaagtg ttttcaccgt attcgaggaa aaagccatcc tggctcaggg cgaattgctc
     8281 tccaccaaca tgtttcagct ctatctggag gagaaaggcg tgaacagcgt tctgcttccg
     8341 gcactggagt acatgcgcat tgataaaaac tcggaacccg ataacgcttt catagctgaa
     8401 aatctgaaaa aactgctgtc agaaaacaag ggtaaagata tttatattac acagggatac
     8461 atctgccgaa atttctacgg tgaaattgac aacctgcaac gcggcggaag cgattactcg
     8521 gcttcactta tcggtgcagc catcaaggca gatgaaattc agatatggac cgatatagac
     8581 ggaatgcaca acaacgatcc gcgctacgtg gagggaacaa aaccggttcc gatgctgcat
     8641 ttcgaagaag cagccgagct ggcctacttc ggtgcaaaaa ttctgcatcc tacctgcatt
     8701 ctcccggcaa aactgcacaa cattccggtt cggttgctca atacgctcga cccttcagct
     8761 ccgggtacaa tcatctctaa caagcgcaaa agaggcatta ttgaagctgt agctgccaag
     8821 gacagtatta cagccattaa aataaaatca ggccatatgc tcctggcata cggttttctg
     8881 aggaaagtgt ttgaaatctt cgaaagcaac cagaccccca ttgatatgat caccacctcc
     8941 gaggtaggag tttcagtaac tattgacaac acccggagac tcgaagagat attaaacgac
     9001 ctgaaaaaat ttggtacggt gaccgtagat aaagatatgg taatcatctg tatcgttggc
     9061 gatatggaat atgaactcgc cggttttcag gcaaaggtca tcaatgcgct gaaagacatc
     9121 cccttacgaa tggtttctta cggaggaagc aatttcaaca tttcggttct gataaaagct
     9181 gaagacaaga aaaaggcttt gaacctgctc agcgaagcct tattcaacta aaaataacga
     9241 accgataggt aacacaatta cttatcggtt ttttaagaat taatttcaaa aaaaagatga
     9301 taaaagccca ttatcccaca gataaattcc aacagataag tactccattt tattattacg
     9361 atctggatgt actccggagc accctgagtg aaataaaaat gcttgatttg cccgaaaact
     9421 acatggtaca ctacgcgatg aaagccaacg tgaatccaag catactggag gtgataaaaa
     9481 atgccggatt cggtgccgac tgtgtaagcg gaggtgaaat tcaggctgca atagacgccg
     9541 gatttccggc atcgaaaatt gtatttgccg gagtgggaaa atccgactgg gaaatcaatt
     9601 tggctttaga acacgatatc ttttgcttca acgccgagtc ggtgcccgaa ctggaagtaa
     9661 tcaatgaact ggctgcctca aaaaacaaaa aagcccgggt agcactgcgc atcaaccccg
     9721 atgtaagtgc tcacactcat aaatacatca ccaccgggct gagtgaaaac aagttcggaa
     9781 tcaacatgga agacctggat catgtcatca gcctgctgcc caaactcact catcttgaat
     9841 ttacaggcat tcattttcat atcggatcgc aaataaccga tatgactgct ttccacaatc
     9901 tgtgcgttcg gatagaggaa attcaggata ttttcaacac aaaaaacatt gagattaccc
     9961 atatcaacgt aggaggcggt ttaggtgtca actacgagca tccgaatcat ttcccggtgc
    10021 cggaattcga aacttacttc aaaacctttc gtgaccacct gggcctgaaa cccggtcaaa
    10081 cactgcattt tgagctcgga cgatcgatag tagcccaatg tggcagcctc atttcacggg
    10141 ttctgtatgt aaaagagggt tcaacacgta aatttgtaat acttgatgcc ggctttaccg
    10201 acctgattcg tccggccttg tatcaggctt atcaccgcat cgagaattta tcctcggaaa
    10261 aaccgacctg tttgtatgat gttgtaggac ccatttgtga atcatccgac gtttttgcca
    10321 aagatttcat gctgaatgaa accaaaagag gggatttgat agccatccgt tcggccggtg
    10381 cctatggcga aatcatggct tcgcagtaca acctgcgcag tttgcctcag gcgtacacat
    10441 ctgatgaatt gaaataacag aagttgaaag aaattattga aaattacatg gatttttttg
    10501 gtataatacg tttctcagtt gttgaaataa gcctgctggc tgtgttgctg actgctttta
    10561 tcgttcaact gtatttttac atccgttatt tcatgggggt aattcgtcac aataaacgtg
    10621 tgtcaaaagg gattgagcag tttcggaacg accagccgcc ggtcagtgtg attatctgcg
    10681 cccgcgacga agaagaaaat ctccggaaat ttctaccttt tgttctggag caggattatc
    10741 cggactacga agtaattgta ataaacgacg gttcgaccga tcatacggat gattatctat
    10801 cgctgatggt gaagcaatac gaaaaactgc gaacaacttt cgtaccgggt ggagctacca
    10861 atgtgagcac caaaaagctg ggcttaacac tgggtatcaa agcggcaaaa aatgatttgc
    10921 ttttattcac ggatgccgat tgtatgcctg agggcaaaaa ctggctcgaa atgatggtac
    10981 gcaatttcac acccgaaacc gaatttgtac tcggctacgg aggttacctt cccaaaaaag
    11041 gatttttgaa taaactcatc aaatacgaca ccctctttat tgcactgcag tacctgggta
    11101 tggctgcttc acacagaccg tatatgggtg tggggagaaa tctggcatat cggaaagata
    11161 ctttcttcaa tctgaaagga ttcgctacca cgcttaatct catgtccggc gacgatgatc
    11221 tgatggtaaa ccgcggtgca actaccgaaa atacaagaat tgaagtgtct tcggaaagcg
    11281 ttacctggtc tgaaccgaaa acaaccttca gaaactggct aattcagaaa gaaagacacc
    11341 tctcagtatc agataaatac aacactccga gtaagctgcg actaactgtt gaacctgttt
    11401 cacggggcat tttctacctt gcttttgtga ctgccgttgt ggtaagcgga ttgagtttca
    11461 actgggttgc agctgcattt gcaggtttgc ttttcatagt gagattcacc ctgcaactct
    11521 taattatcaa caaatcggct aaaattctcg gtgaacgaag attttatttt accatacctc
    11581 tttttgacag tattttaccg ctgataaccc tttctattct cactttcgga aagaaaaaaa
    11641 gaaacttaaa atggaaatag ttttcttttg aatgtttgaa tgtttggatg tttggatgtt
    11701 tgaatgtttg tcccgatagc tatcgggagt ttggatgttt gaaaattatt ctgtttattt
    11761 ttaaaaacag agtttggaat ttcgagaaaa aactcaaaac tccaaactct ttactccttg
    11821 ctttttagtc caaactcaga actgattttt tcctattccc agctcagaat aacttttcca
    11881 cactgcccgc tttccattat atcaaatccc tgttggaact catcaatacc gaaacgatgt
    11941 gtaatcaccg gattcaaatc gaacccggat ataagcattt gctccatttt gtaccaggtt
    12001 tcccacattt ctcgtccgta gatgcccttc agagtcaggg ctttgaaaat aatcttgttc
    12061 caatcgactg ttgtagtatt cggcagaatt ccgagaagtg aaattttaga accgttgtac
    12121 atattatcaa tcatatcccg gaaagcagtt ggggctccgg acatttcgag cccaacgtca
    12181 aatccgcgca tatgaagttc tttcattgca ccctgaatag tttcgccttt ggaaggattg
    12241 accgtgaggg ttgctcccat ctttttggca atttcgagcc ggtaatcgct aaaatcgctt
    12301 accaccacat agcgagcccc ggcaaaccgg cagattccgg tggccatggt tccgataagc
    12361 cctgcgccgg taatcagtac gtcttcgcct atcagtggaa acgacaatgc cgtatgtgta
    12421 gcatttccaa acggatccat gatggaggct gtttcgtcag aaatgcggtc gtccaggtga
    12481 acaacattgg aagccggaag cgaaagatat tcggcaaagg ctccatcgcg atttacacct
    12541 acaccaatgg tgttttcgca tacatgcagt tttccgcggc ggcaattgcg gcaatgcccg
    12601 caggcgatgt gtccttcgcc ggtcacacgg tcgccaatct tcacattctt tactcccctg
    12661 cccttttcca caactacacc aacgtattca tgtccgatag tcatgggcac tttgattgta
    12721 ttctgcgccc agtcgtccca tttatagata tgtaaatcgg taccgcaaat ggatgttttt
    12781 ttgattttaa taagtacatc gttaattccg atttcaggca ccggtatatc ttccatccaa
    12841 atgccttttt ctgcttttgc ttttactaat gctttcatat atagatattt acattattta
    12901 ttcttgattt tttttaaaga taaaatcatt aacactatcc gatcacaccc agttctttac
    12961 caactttcgt aaatgcatcg atgcatgtat ccagatgagt tttttcgtga gctgccgaaa
    13021 tctgaacccg gatgcgcgct tctcctttcg gtacgacggg atagtaaaac ccgatgacat
    13081 agattccttc atccagcaat cggttggcta cttcctgtga taattttgca tcgtaaagca
    13141 tcacagcgca gatggctgat tcggtaggct tgatatcgaa accggcggct ttcatttttg
    13201 caacaaaata ttctgtattt gcgtggagtt tgtcctgcag gtcgttggtt tcatccatca
    13261 tctcgaacat gcggattccg gctgctgcca ccatgggagg tatggaattg gaaaataaat
    13321 atggtcgcga acgttgacgc agcaaatcga tgatttcttt ttttccggtg gtaaaaccgc
    13381 cgatggcgcc gccgaaagcc tttccgagtg ttccggtgat gatttctatt tttcctttga
    13441 ggttgtagcg ttctgtcacg ccccttcctg tctgaccaac cactcctgca gaatgcgatt
    13501 cgtcaaccat caccattgcg ttatattttt cggctaattc gtaaattttg tccagaggag
    13561 ccacatttcc gtccatagag aaaaccccgt cggttacgat taacctgaaa cgttgggcct
    13621 gtgccttttt caactgttct tccaggtcgg ccatatcggc attggcatag cggtagcgct
    13681 gagctttgca cagtcttact ccgtcaatga tggatgcgtg attgagcgca tcggagatga
    13741 tggcgtcttc agccgaaaga agtggttcga atacgccacc gttggcgtca aaacatgctg
    13801 cgtagaggat ggtatcttcc gttccgaaaa aacgggcaat ggatgtttcg agttgcttgt
    13861 gaagatcttg tgtgccacaa ataaagcgaa cggatgacat gccgtaaccg tgagtatcta
    13921 tggctgactt ggctgcatct ttcagctgct gattgttgga gaggccaaga taattgttgg
    13981 cgcagaaatt tagcacttct ttgccgtcag aaacccggat ggctgcaccc tgaggagaaa
    14041 ctatgattct ttcatttttg tacaatccgg cattttcaat gccttgtaat tcactctgta
    14101 agaatgattt aaaattttga tacatatata tttgaattta aagttgttct gtcgtaatgc
    14161 attacaaagg taatgaatta atcttaaaag atatttaaga aaggaagata tgttgcttta
    14221 gtggcaggca ctaaaacaat caggacaaga aactgattat caaactgtta agacacatca
    14281 tactaaaaga aactcatttt tttttaattt catttccggg gaggataatt gagctgattt
    14341 catctatttc gattttctca aacccgagtt cttcaagcag ggatgtgatg aacttgtgag
    14401 cctgatcgga cgattcggtg agtattcctt tcgcaagcgc atcctgtttg attttatgct
    14461 gttcggctac catagcattt tgatagtcag tcactttaat aggattcagt atattcatgc
    14521 tttcgtcata cacctgaaga gatttttcat caattacggt ttcctgaatc tgcggtttag
    14581 gcagaccgat ttttacactc ttcccgttga cagttacagt ggctttgctc atgtcaatcc
    14641 cggctttcac atagccgttg tatctgatca gaaacgactt ttcggtgagc ggtacctgaa
    14701 gattcatgaa tttcatgtaa tctttgtagc tgatcacgcc gctatagttg tacttcacca
    14761 atgagagatc tttgacgtat gaaatcttct ccataatggc actgctgttg taagccgttt
    14821 tttgaggaat gtattttgca cggtaaagca aaactgacag caatacagtg attccaagca
    14881 gcacccatgc aatgtaatta tgtcttctca tttactttgt tttattattt ggatggggta
    14941 atataggata tacagtttca ttaatccata aaaagatcat tagcctgtca ccagaaatag
    15001 caagagcaca agcaagacag ttgctcccac cacagagcca aaaattatga gcatcagttt
    15061 gaaaaattgt ttgttttcca tttcaatcat tttttaaaat accaaacaaa gataattatt
    15121 tttgttgttc ttgtgtactt gttgtcggta aaataaacat tgttattact cagcccccac
    15181 ccccccaaag ggggagttct ggagtaacta acaacatgta tgcatttgaa tttggtagtt
    15241 tttttagaaa atatttaatt ttattgaaac cgcaattgtt ctataaatga gtctgctccg
    15301 aaaaccttgt ttttgccaca aagacactat aacaccaagt tacacaaaga aactaatttt
    15361 atatctaatt gattatcaat acatc
//
LOCUS       DAORKP010000038        14915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_313_length_14915_cov_9.110767, whole genome shotgun sequence.
ACCESSION   DAORKP010000038 DAORKP010000000
VERSION     DAORKP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_14915_cov_9.110767"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLN75_03575"
     CDS             complement(<1..299)
                     /locus_tag="PLN75_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRG03230.1"
                     /translation="MKHLQVAALQNGTVIDHIPADKLFKVFSILNLESCSETITVGNN
                     LNSDTLGKKGIIKISDRFFEEDETNKIALIAPKAKINIIKEYEVVEKRPLELPEDV"
     gene            complement(355..711)
                     /locus_tag="PLN75_03580"
     CDS             complement(355..711)
                     /locus_tag="PLN75_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRG03231.1"
                     /translation="MKNLLETGNRIVDLTFGFSLKTIEYVELLEEKRKFVIANQLLKS
                     GTSIGANIREAQYCESKADFIHKFKMAAKEAEEIEYWLLLCKYSNSYPFEEKLLEEVK
                     EIQRIINSIINTSKTK"
     gene            complement(832..1737)
                     /gene="pyrB"
                     /locus_tag="PLN75_03585"
     CDS             complement(832..1737)
                     /gene="pyrB"
                     /locus_tag="PLN75_03585"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRG03232.1"
                     /translation="MKNHSLVSISDYSKEEILEILHSASEFEANPNRKTLDGKVIATL
                     FFEPSTRTRLSFETAVNRLGGRIIGFSDSTTTSSVKGETLHDTIRMVSCYADAIVMRH
                     PLEGAARYASEVSPVPIINAGDGANQHPSQTLLDLYSILKTQGTLENLKICLVGDLKY
                     GRTVHSLIMAMSHFNPSFRFIAPNELKMPEEYKIFCRKCGIPYEETADLAGNFDDADI
                     LYITRVQKERFLDLIEFERVKNVYTLKNSMLENTKPNLKIMHPLPRVNEIDQDVDEND
                     KAYYFQQAQNGLYVRQAIMSKVIFP"
     gene            complement(2033..2749)
                     /locus_tag="PLN75_03590"
     CDS             complement(2033..2749)
                     /locus_tag="PLN75_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRG03233.1"
                     /translation="MKVEIWSDVACPFCYIGKRHFEKALSQFTEKEHIEIEWKSYLLD
                     PEYVYDPENPVTETEYIAARKGISQDEARQMFTSITRMASMAGLTYDFDKVIVANTFD
                     SHKIIQLAKEKKLGDAAEERFFQAFFVNGENLNEKETLVKIAFETGLTESDVNEALTN
                     DKYAYEVKNDIQEASQIGVRGVPFFVFDRKYGVSGAQPVTTFTQTLEKSFAEWKEKNQ
                     LNPLINVAEGSACDIDGNCE"
     gene            complement(2819..3484)
                     /locus_tag="PLN75_03595"
     CDS             complement(2819..3484)
                     /locus_tag="PLN75_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRG03234.1"
                     /translation="MGAEIPQNYFQIIIQSILSIINSFITISGISEGIYKEKGSKFLS
                     FALPVTTVEEVKVILDEYRKKFYDARHVCYAYMLGAERADFRANDDGEPSGTAGRPIL
                     GQINSFELTDVLVIVVRYFGGILLGTGGLITAYKEAARDALSQAEIVEKVITQRFEIR
                     FGYELMNEVLRVFKEFEPEILKSELQYDCRYVVDVPKASSEQFFQKMNTIYGVKIERT
                     NMK"
     gene            complement(3448..3975)
                     /locus_tag="PLN75_03600"
     CDS             complement(3448..3975)
                     /locus_tag="PLN75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRG03235.1"
                     /translation="MRKIILLFAIITTFSVAAQTPVDTISTFQYPAKEVIPKPYNPKE
                     DAAKKINELLVQAKKEQKNIFIQAGGNWCGWCLRFNYFVQNTPELKQIIDQNYLYYHL
                     NYSPENKNEKVFRKYTDPDKKYGYPFFIILSSDGKILTVQESGILEAGKGYNVEKVKN
                     FLERWVPKSPKITSK"
     gene            complement(3997..5304)
                     /gene="murA"
                     /locus_tag="PLN75_03605"
     CDS             complement(3997..5304)
                     /gene="murA"
                     /locus_tag="PLN75_03605"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRG03236.1"
                     /translation="MSSFKIEGGQKLGGSITPQGAKNEALQVICATLLTPEKIIIRNI
                     PEILDVLNLIDLLKDFGVKVEKITADTYFFQADEIDLNYLESDDFYKKSAALRGSVML
                     VGPLVARFGIAAIPKPGGDKIGRRRLDTHFLGIQKLGADFEYDADLKIYKISAKVLKG
                     CYMLLDEASVTGTANILMTAVLAEGETTIYNAACEPYLQQLSKMLNRMGAKISGVGSN
                     LLTVQGVKSLKGCDHTLLPDMIEVGSFIGMAAMTGSEITIKNVAFKELGIIPDSFRRM
                     GIKIEQHGDDIFIPHQSSYTIESFIDGSIMTIADAPWPGLTPDLLSVFLVIATRANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGKHLHLRAAKMSSPDIRAGI
                     ALLIAAMAANGTSIIENIEQIDRGYQEIDKRLNAIGAKITRIS"
     gene            complement(5482..6072)
                     /locus_tag="PLN75_03610"
     CDS             complement(5482..6072)
                     /locus_tag="PLN75_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRG03237.1"
                     /translation="MEYPTLQGKLIMPEYGRNVQQMIEYALKIEDREERTKCVNTIIN
                     VMGSLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDYPYEIVKKEEMYERPETIPYKNSR
                     ISYMHYGRNLEEMIERISDYPDDDTKKELIRMIANQMKKCFLSWNKEVVDDRKIFDDL
                     RELSKGKIDVAEDIFKLMETKDVLHSKKVQNKPKKK"
     gene            complement(6115..7164)
                     /locus_tag="PLN75_03615"
     CDS             complement(6115..7164)
                     /locus_tag="PLN75_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRG03238.1"
                     /translation="MRKFIYLIVFTFVFAACGTGKKVESTNSGGNFQVVSVAFYNLEN
                     LFDTINQSNIDEEFLPDGAMRWTGMKYKAKLKNMSYVISQLGKDITPGGPSIIGVSEI
                     ENRGVLEDLVKQPSIASRTYEIVHYDSPDRRGVDVGLLYNPRHFIVTNSKSCVLQTND
                     TAFHTRDQLVVSGYLMNEKVHVIVNHWPSRTGGEVASAPRRNAAAGLTKQIADSLYAQ
                     DANAKIIIMGDLNDDPFNESTAKILNAKRNMEDVPLKGLYNPMWRILDSGVGSLAYNN
                     QWNLFDQIIISQPLLTKEKTKLSFWKAEVFNKSFLIQQEGSYKGTPMRTHAGGVWLNG
                     YSDHFPTLIYLVKEK"
     gene            7572..10340
                     /locus_tag="PLN75_03620"
     CDS             7572..10340
                     /locus_tag="PLN75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG03239.1"
                     /translation="MRKIWLVFAAFIFTTAFLSAQTRLSGYVKIAESETPLPGVTVTL
                     LHQNISTKTNAQGEFNFSYLTAGDEEVSFSKSGYLPQIKLISLKDDTQNDFGTVFLRV
                     DIQEESRQDAILQLNESDFSEDEGRASQSISTSLSRGDVYLSQSSYNFSPMRFRMRGY
                     EQEFESTYVNGVHFNALDRGGFNYSSLGGLNDANRNKDVVVGSEANTFSYGNLGSNTN
                     ILNRASAFAAGTRASVAYSNRAYKLRGQVTYATGLMPNGWAFAASGVFRWADEGLAQG
                     TFYNSAGYFFSAEKMLTPRHSLSLITYGAPTRRAQQSAATQEVYDMTGTIYYNSYWGY
                     QEGVKRNSRIVKSFDPTAIIASDFKISETQRLRTGLAYHYSMYSNSALSFYNAPDPRP
                     DYYRNLPSFQTDETIAEQIRKEWAFDNGDPVNDKVSQIDWESLYSANYKNNEINPAGN
                     AKYAVERRHNDLMETAFNSTYTNQLNPNLRLTAGIEAKMAKDINYKTMDDLLGANQWI
                     DIDQFAERDLNIDPNPDPNKIQNDLNNPNKVIKNGDLFGYKYNINIIHSSAFIQNEWN
                     LSQLDIYYAAQLTYTQFNRHGYMRNGRAPENSFGAGQIWWFVDPSVKAGATYKIDGRN
                     RLYANVLAETRAPLPSGAYVSERIKDDRIPYLQSRKILSYDLNYSFIFPSVRGRFTGF
                     RTHMLGTSENLGYYDDALRTFINHMLMKSDKIYQGIEAGLSVKLNRSFSISTAGTFAD
                     YHYTNDAIGVKSFENGSKADIQETVLTKGLKINTGPQLAANITLDYFHPKMWFADVTL
                     NYFDNNYLDFAPNRFTASNVAMYEADATGEAKRILGTQEKLNAGFLLDASIGKVVYLP
                     KRRSMNINLSFSNILNNTNMITGGFQQGRMPMLDETSLDMANLNRFPNKYYYAWGFNM
                     FLNLGFRF"
     gene            10367..11353
                     /locus_tag="PLN75_03625"
     CDS             10367..11353
                     /locus_tag="PLN75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRG03240.1"
                     /translation="MKIKSLIYIIVPVVLLTSCNKTEFDAIPVSELMGDSLKTTYTIE
                     QLKTDFMTNSDAYSDPVGKMAGLYTADKINSSQDVVISGFVTSTDVEGNVYKYFVVQE
                     DKPNGQALKVSIDASGLSAVYPLGQQVWIRCNDLYIGKYGEAPQLGSLYINKERMKIS
                     GADTIYRTEPGRMPLFTAQKHVYSYGMPQPDRIKADTMTVAQIKAADYKKLVNKLVCI
                     KNAYFTGFDGNKKALSSQNMIFAPSTGGVGYPQLRNISDGTGDVAIATSEYAKFATKP
                     LPPSNFSGNITVIVGWYKNYTDAAGVYQLTLRTLDDLGKGFETYLSNINYTK"
     gene            11370..12851
                     /locus_tag="PLN75_03630"
     CDS             11370..12851
                     /locus_tag="PLN75_03630"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRG03241.1"
                     /translation="MKKKLIYGFLLLTASVITACTNATDYDLENPESQKVIFTETFES
                     GTLGDFTTQSVTGDQVWVGDARGYAFMTGYVSATRENIANEDWLISPQINLTDVTTAH
                     FTFEHVARYFADLVNEATVWISDNYVSGAPSTATWTQVVTSPFRDPGAWDFQPSGQLS
                     LTAYAGKKIHIAFKYISTNTKAGSWEIKNMVVATGEAVNENVNYGLGTAASPYSVLGG
                     TYNQNNTSAWLKGTIVGYVWYVAGKGNSFYFSGDTCTQATNILVADSGSLYLSQAMAV
                     QLPAGAVRTGINLKDNKSLYGKKVTLYGDLTAYFGIAGLKNTSYYKLEDGTTGGNKPF
                     DPSTAIYYETFATNLGKFTTQNVTGGEIWTYNGTYKCAYMTGFVSSQNRANEDWLISP
                     EIDLTGKSDIALAFDHVFRYGGEPANEATVWVSENYSSGLPATATWTQITTNFTNAST
                     WDFYNTGDISLKAYAGKKIKIALVYKSTSTKAGTWEVKNFIVK"
     gene            complement(12955..14481)
                     /gene="gpmI"
                     /locus_tag="PLN75_03635"
     CDS             complement(12955..14481)
                     /gene="gpmI"
                     /locus_tag="PLN75_03635"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRG03242.1"
                     /translation="MRKKALLMILDGWGIGNQSEADLIYNTPTPYWDKLLATYPNSYL
                     LASGENVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINRACRDNSIMQNPEIVKAYTY
                     ARDNGKKIHLLGLVSDGGVHSSLDHLMKLIDIANEYGVTDTYVHAFMDGRDTDPKSGK
                     GFIEQLQNHIDQTTGKIATIIGRYYAMDRDKRWERVKQAYDLLVNGVGTPASDMVQAM
                     QDSYDEGVTDEFIKPIVHVDSDGKPVAKIEEGDVVIFFNFRNDRAKELTIVLTQEDMP
                     EHGMHTIPLQYFTMTPYDSSFKGLHVLFDKDNVQNTMGEYVSGLGLKQLRVAETEKFA
                     HVTFFFSGGREAEFPGEERLLVPSPKVATYDLQPEMSAPEIADRMVDALNSQKFDFIV
                     LNFANGDMVGHTGVKEAIVKAVETIDVCVEKVIEAAKANDYEAIIIADHGNADNAINA
                     DGSPNTAHSLNPVPFVFVTNDKNATIEPGILADVSPTVCQVLGIDIPKEMSGRGLITF
                     "
     gene            14693..>14915
                     /locus_tag="PLN75_03640"
     CDS             14693..>14915
                     /locus_tag="PLN75_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HRG03243.1"
                     /translation="MFIVLSFMLAGIIVGYLLRSKKIRFFQGLIITLIWLLLFLLGLE
                     IGANKEVVSQFGKLGLEAFIIAAGGTLGSV"
BASE COUNT         4112 a         3230 c         3061 g         4512 t
ORIGIN      
        1 acatcctcgg gtaattcaag cgggcgtttt tccaccactt cgtactcctt gataatgttg
       61 attttggctt ttggagcaat caacgcaatt ttattggttt catcttcttc aaaaaaacgg
      121 tcggagattt tgataattcc ctttttacca agcgtatcac tgttcagatt attcccaact
      181 gtgatagttt cactgcagct ttccaaattc aagatggaaa aaactttaaa taatttatcg
      241 gcgggaatgt gatctatcac tgttccgttt tgaagtgctg ctacttgtag atgtttcatg
      301 atttaatgtg ccaatttgat aatatgccaa tatgccaatt tgcgtattga atctttattt
      361 ggttttactt gtattaataa tgctgttaat gatacgttga atttctttga cttcctcaag
      421 tagtttctct tcaaaaggat aggaatttga gtacttacat aacaatagcc aatattcaat
      481 ttcttctgcc tcttttgcag ccattttaaa cttatgaata aaatctgcct tgctttcaca
      541 atattgtgct tctcgaatat tggcaccaat ggatgttcca cttttcaaaa gctgattggc
      601 aattacaaac ttacgctttt cttccagaag ttcaacgtat tcaattgttt ttaaagaaaa
      661 tccaaaagtt aaatcaacta ttcgattacc cgtttctagt agatttttca tacctaaact
      721 caattaaaca attctgtaat aaaacagctt ttttccgatt gataaagtgt tcaatttgca
      781 tattgccaca ttcatgcatt ggcatattgg cacattatat taattggcat attacggaaa
      841 aataacttta ctcataattg cctgcctcac atatagcccg ttttgtgcct gctgaaaata
      901 gtaagctttg tcgttctcat ccacatcctg atcaatttca ttcactcgcg gaagcggatg
      961 catgattttc agattgggtt tggtgttttc cagcatgctg tttttcaggg tgtaaacgtt
     1021 tttcacccgt tcgaattcaa tcaaatcgag aaaacgttct ttctgtacac gagtgatata
     1081 cagaatatcc gcatcatcaa aatttccggc taaatcggct gtttcttcgt agggaatgcc
     1141 acatttgcgg cagaatattt tgtattcttc cggcattttc aattcgttgg gagcaataaa
     1201 ccgaaacgac ggattgaaat gcgacatggc catgataagc gagtgaaccg tgcgtccgta
     1261 tttcaaatcg ccaacgaggc agattttcag gttctcgagc gtgccctgcg ttttcaggat
     1321 ggagtaaaga tcaagcagcg tttgcgacgg atgctgattg gctccgtcgc cggcattgat
     1381 tatcggcacg ggtgatactt cggatgcata acgggctgcc ccttcgagcg gatgccgcat
     1441 gacgatggca tcggcatagc agcttaccat tcggatggtg tcgtggagtg tttctccttt
     1501 caccgaactc gtagtcgttg agtccgaaaa tccaataatg cgccctccaa gccggtttac
     1561 ggcagtttcg aaactcaaac gggttcgggt ggacggctcg aaaaaaagtg tggcaatcac
     1621 tttaccatcc aaagttttgc gattcggatt tgcttcgaat tctgatgctg aatgcagaat
     1681 ttcaagtatt tcctctttgc tgtaatcgct gattgatacc aaactgtgat ttttcataag
     1741 ccccttaatc ccccggaagg ggattttttt caatggttat tgtttttttt tagaaaaaaa
     1801 ccaacattat tcgcagggaa taagttggct tcaatttttt taaggataaa ttttctgaac
     1861 aacttgaatg ttgaaagttg ccaacgaaaa tagaatattg attatgtctg ctaatcatat
     1921 tgaataccgc aaagatactt gtttttttct gtaaaattca aacctttgtc tgaaaaactt
     1981 ggttgaattt taataaaaaa aaccggccgg aaaggtcggt tttggatgtt ttttattcgc
     2041 agttgccgtc gatatcgcag gcactgcctt ctgctacatt gataagtgga tttaattgat
     2101 tcttttcttt ccactctgcg aatgattttt cgagtgtttg ggtgaatgtt gtcaccggtt
     2161 gcgctcccga tacgccgtat ttacgatcaa aaacaaagaa tggaacgccg cgtacgccaa
     2221 tctggcttgc ttcctgaata tcgtttttta cctcataggc atacttatca tttgtgagcg
     2281 cttcattcac atcgctttcg gtaaggcctg tctcaaacgc tattttcaca agcgtttcct
     2341 tttcgttcag gttttcacca tttacgaaaa atgcctgaaa aaagcgttct tcagccgcat
     2401 cgcccagttt tttttcttta gccagttgaa taattttatg tgagtcgaac gtattggcta
     2461 caatgacctt atcgaagtcg tatgtcaaac cggccataga agccatgcgc gtaatacttg
     2521 tgaacatttg gcgggcttca tcctgcgaaa ttcctttgcg cgcggctatg tattctgtct
     2581 cggttaccgg attttctgga tcataaacat actcaggatc aagaagatac gatttccatt
     2641 ctatctcaat atgttctttc tcagtaaatt gcgataatgc tttttcaaaa tgacgttttc
     2701 ctatatagca aaacggacat gctacatcgc tccatatttc tactttcatg attttgtttg
     2761 tttttagtaa tgtttcaaaa aaacaatcga actcgcctga aagttttatc aatggcatct
     2821 atttcatatt tgtccgttcg atttttactc cgtaaattgt attcatcttt tgaaaaaact
     2881 gctctgatga tgctttgggt acatccacta cgtagcggca atcgtactga agttcagatt
     2941 tcagaatttc aggttcaaat tctttgaata cccttaaaac ttcgttcatt agttcgtaac
     3001 caaagcgaat ttcaaaacgt tgagttatta ctttttccac aatttcagcc tgagaaagtg
     3061 catctcttgc tgcttctttg taggccgtaa tcagtcctcc ggttccgagt aagatgccac
     3121 cgaaataacg cactacgata accagcacgt cggtcagttc gaaggaatta atctgcccca
     3181 ggatgggtcg tccggccgtt ccggaaggtt ctccgtcatc attggctctg aagtctgccc
     3241 gctcagcacc aagcatatag gcatagcaca cgtggcgggc atcgtagaat tttttccggt
     3301 attcatccag aatcactttt acctcctcca cggtggttac aggcaaagca aaagaaagga
     3361 atttactccc tttttctttg taaattcctt cggaaatgcc tgatatggtg atgaaagagt
     3421 ttatgataga aagaattgat tgtataatta tttggaagta attttggggg atttcggcac
     3481 ccatctctca agaaaattct ttactttttc cacattatat ccttttccgg cttccaaaat
     3541 gccgctttcc tgtacggtca atatttttcc gtcgcttgat aaaatgatga aaaacggata
     3601 tccgtacttt ttatcaggat cggtgtattt tctgaatact ttttcgtttt tgttttcagg
     3661 cgaatagttc aggtgatagt acaggtagtt ttggtcaata atttgcttta attcaggggt
     3721 gttttgaacg aaatagttga agcgcagaca ccagccacac cagttgccac cggcctgaat
     3781 gaaaatgttt ttttgttctt ttttggcttg caccaacaac tcgtttatct tttttgcagc
     3841 atcttccttc gggttgtaag gctttggaat gacttcttta gccggatatt gaaaggtaga
     3901 aatggtgtca accggtgttt gtgccgcgac agaaaatgtt gtaataattg caaaaagaag
     3961 aattatcttt ctcatagtca ataatatata cctatcttat gaaattcgtg tgatttttgc
     4021 gccaatagca ttcagccgtt tgtcgatttc ctgatatccg cggtcaatct gctcaatatt
     4081 ctcaatgata ctggtgccat tggctgccat cgctgcaatg agcaaagcga tacctgcccg
     4141 gatatcaggc gaactcattt tggccgccct gagatggagg tgtttgccaa gtccgatgac
     4201 ggtagccctg tgcggatcgc aaagtatgat ttgagctccc atgtcaatca gtttatccac
     4261 gaaaaacaga cggctttcaa acattttctg atgaatgagt acgctgccgt ttgccctggt
     4321 ggcaataacc agaaaaacac tcagcaagtc gggagtcagt ccgggccagg gagcatccgc
     4381 aatggtcata attgaaccgt cgataaaaga ttcgatggtg taggaactct gatgtggaat
     4441 gaaaatatcg tcgccgtgtt gctcgatttt aatacccata cggcggaaac tatccggaat
     4501 tatgcccagc tccttgaacg caacgttttt gatggtgatt tcagaaccgg tcatggcggc
     4561 catgccgata aaacttccaa cttcaatcat gtcgggcagg agcgtatgat cacatccttt
     4621 gagcgatttc actccctgaa cggtaagcag attggatcct acgccactta ttttggcacc
     4681 catgcggttg agcattttac ttaattgctg caggtagggt tcgcacgcag cgttgtaaat
     4741 ggtcgtttca ccttcggcca gcacagccgt catgaggata ttggcagtac cggttaccga
     4801 tgcctcgtcg agcagcatgt aacagccttt cagtactttc gcgctgattt tataaatctt
     4861 caggtcggca tcgtattcaa aatcggcacc cagtttctga atacccagaa agtgagtatc
     4921 taaccttctg cggcctattt tatctccgcc cggtttcggg atggcagcta taccgaatcg
     4981 ggcaaccagt gggcctacca gcataacgga acctctgaga gcggcacttt ttttgtaaaa
     5041 atcgtctgat tccaggtaat tcagatctat ctcgtctgcc tgaaaaaaat aggtgtcagc
     5101 cgtaattttc tccactttca cgccaaaatc tttcagcaaa tcaatcagat tcagcacgtc
     5161 aagaatttcg ggaatatttc ggatgattat cttttcggga gttagaagtg tggcacagat
     5221 gacctgtaag gcttcatttt ttgcaccttg cggagtgatg gagccaccaa gtttttgtcc
     5281 tccctcaatt ttgaatgatg acattttttt aagttagaag ttagaaatta gaagtcaaaa
     5341 gtcagaagtc aaaagtcaga tgcttgcctg gtttatccgt acttgtcggg gtttccggca
     5401 ggtttgaaat cagaagtaag aaaagagacc gatttgtgtt aacaaagatt ttttccacgc
     5461 acacatttct tatcttgttt tttatttctt tttgggtttg ttttgtactt tttttgaatg
     5521 tagcacgtct ttggtttcca tcagtttgaa gatatcctcg gctacatcta tttttccctt
     5581 agagagttcc cgcagatcgt caaaaatttt tctgtcgtcc actacttcct tgttccaact
     5641 cagaaaacat tttttcatct gattggcaat catgcgaata agttcttttt ttgtgtcgtc
     5701 atccggatag tctgatatcc gttctatcat ttcttccaga tttcttccgt aatgcatgta
     5761 acttatccgg gagtttttgt agggaatggt ttccggacgt tcgtacatct cttctttttt
     5821 tactatttca tacggataat cgatatccag tttgaaatcg gacatgatag ccacatgatc
     5881 ccagagtttg tgtttgaaat catttacatc gcgcaggtag gggaaaagac tacccataac
     5941 atttattatg gtatttacgc atttggttcg ttcttcgcgg tcttcaattt tcagtgcata
     6001 ttctatcatt tgttgcacat tccgaccgta ttcgggcatg attaattttc cctgaagtgt
     6061 ggggtattcc ataattggtt ttttagtctg tggtcaggtg ttttctttac tttattattt
     6121 ttctttcaca aggtagatga gggtagggaa gtggtcgcta taaccgttga gccatactcc
     6181 gccagcgtgt gttcgcatgg gagttccctt gtaacttcct tcctgctgaa tgagaaaact
     6241 cttgttgaat acttcggctt tccagaaaga taattttgtt ttttccttgg ttagtaaggg
     6301 ctgcgaaatg atgatctgat caaaaagatt ccattgattg ttgtaagcca gcgagcctac
     6361 gccactgtcc agaattcgcc acatcggatt gtagagtcct ttgagtggta catcttccat
     6421 atttcttttt gcattgagaa tttttgcggt gctttcgttg aacggatcat cgttcaggtc
     6481 gcccatgatg atgatttttg catttgcatc ttgtgcgtac agcgaatcag caatctgttt
     6541 tgtcaatccg gcagcagcat ttctgcgggg tgcagaggca acctcaccgc cagtacgtga
     6601 aggccagtga ttaacaatta cgtgaacttt ttcattcatc aggtatccgc ttaccaccaa
     6661 ctgatcacgt gtatgaaagg ctgtatcgtt cgtctgcaat acacaagatt tgctgttagt
     6721 aacaataaaa tgtcttggat tgtagagcag gcctacatct actccgcgtc ggtcggggct
     6781 gtcgtaatgc actatttcgt aggtacggct ggctatcgag ggttgtttca ccaggtcttc
     6841 gagcactccg cggttctcta tttcggatac tccgatgata gacggaccgc cgggggtaat
     6901 gtctttgcca agctgcgaga tgacgtagga catgtttttc agtttggcct tgtatttcat
     6961 tccggtccat ctcatggctc cgtcggggag aaactcctca tcgatgttgc tctgattgat
     7021 tgtgtcaaaa agattttcga gattgtaaaa agccacactt accacctgaa aatttccgcc
     7081 tgagttggtt gattcaactt tttttccggt gccacatgct gcaaaaacga atgtaaaaac
     7141 aattaggtaa atgaattttc gcataatatt gatggattac atgtctttat atattttttt
     7201 tcaaaaaaca aaagtccgaa aaaaatatga gataaaaaaa caattcacca aaaaaccttt
     7261 agtttaacct gtgtaaactc agaaagattt ttagctgtat ttacttcaaa atttcatttc
     7321 atcagttatt gttttaatgt caaaatgaca ctaacttatt cacacttctc tccattttca
     7381 attaatttga tttgatatat tgaaaaatgc gacttatgtg cttgttttgg gaaaatgtat
     7441 gtcagaaatg aggtgttaat cgacttctga aatatagctt caaatcatgt gtatttcaaa
     7501 aaaatgtcgt aacttgcaaa actttttaaa attaaattta attaattaac taacagaata
     7561 aaagttcatt tatgagaaaa atttggttgg tatttgcagc tttcattttc acaacggctt
     7621 ttttgtctgc tcagaccaga ctctcgggct acgtgaaaat agctgaatca gaaacgcctc
     7681 tgcccggcgt aacagtaacg ttgttgcatc agaatatttc tacgaaaacg aatgctcagg
     7741 gcgagtttaa tttttcatat ctcacggccg gcgacgaaga ggttagtttt tcaaagtcgg
     7801 gttatctgcc acagataaag ctaatcagcc tgaaagatga tactcaaaac gattttggta
     7861 ctgtattcct gcgtgttgat atccaggaag aatcccgtca ggatgccatt ctacagctga
     7921 acgaatctga cttcagcgaa gacgaaggcc gcgcttcaca gtccatttcc accagccttt
     7981 caagaggtga tgtgtatctc tcgcagtcgt cctacaattt cagccccatg cgtttccgga
     8041 tgcgcggata cgaacaggaa tttgaatcta cctacgtgaa cggcgttcat ttcaatgcac
     8101 tcgacagagg tggattcaac tattcaagcc ttggtggtct gaacgatgcc aacagaaata
     8161 aagacgtagt agtaggttcc gaagcaaata ctttctctta cggtaatctg ggaagcaaca
     8221 ccaatatact gaaccgcgcc agcgcctttg ccgcaggcac acgggcaagt gtggcataca
     8281 gcaaccgtgc ttacaaactg cgcggacagg ttacttatgc caccggtttg atgcccaacg
     8341 gatgggcatt tgcagcatcg ggcgtatttc gctgggccga cgaaggttta gcccagggta
     8401 cattctacaa ttcggccgga tatttcttct cagctgaaaa aatgttgact ccccggcaca
     8461 gcctctcact gataacctac ggcgctccta ccaggcgcgc tcagcaaagt gcagccaccc
     8521 aggaagtgta tgacatgacg ggtacaattt attataattc ttactgggga tatcaggaag
     8581 gtgtaaaacg taactcccga atcgtgaaaa gtttcgatcc gactgccatc atcgcttccg
     8641 atttcaaaat aagcgaaacc cagcgactcc gcaccggact ggcatatcac tacagcatgt
     8701 acagcaattc tgctctcagc ttctacaatg ctcctgatcc gcgtccggat tactaccgga
     8761 atttacccag ttttcagaca gatgaaacca tcgctgaaca gattcgcaaa gaatgggcat
     8821 ttgataacgg agatccggta aatgataagg tttctcaaat cgattgggaa tcattgtatt
     8881 cggctaacta caaaaacaac gagatcaatc cggccggaaa cgccaagtat gcagtggaac
     8941 gccgccacaa cgatctgatg gaaactgctt ttaactccac ctataccaac cagctaaatc
     9001 cgaatcttcg cctgacagca ggtatcgaag ctaaaatggc caaagatatt aattataaaa
     9061 ccatggacga tctgcttgga gctaaccaat ggattgatat cgaccagttt gccgaacgtg
     9121 atttgaatat cgatccgaat ccggatccga ataaaattca gaacgacctc aataatccta
     9181 acaaagtgat taaaaacggc gatttgttcg gttataaata caatattaat atcatccaca
     9241 gcagcgcatt tattcaaaac gaatggaacc tgtcgcagct ggatatttat tatgccgcac
     9301 aactcactta tactcagttc aatcgtcacg gctacatgcg caacggacgc gcccccgaaa
     9361 attcattcgg agccggtcag atctggtggt tcgttgaccc ttcggtaaaa gccggagcta
     9421 cctacaaaat cgatggacgc aaccgcctct atgccaatgt acttgccgaa acgcgtgctc
     9481 cactgccaag cggtgcatac gtatccgaaa gaatcaaaga cgaccggatt ccttatttgc
     9541 aatcacgtaa aattctttcg tacgacttaa actactcgtt cattttcccg tcggtacgtg
     9601 gcagattcac cggattccgt acccacatgc tcggaacttc tgaaaacctg ggttattacg
     9661 acgatgcact tcgcacattt atcaaccaca tgctgatgaa atcagataaa atctatcagg
     9721 gaattgaagc cggtctctcc gtaaaactga acagaagttt ctctatctca actgccggca
     9781 cttttgccga ttatcattat acgaatgatg ccattggtgt taaaagtttt gaaaatggtt
     9841 cgaaagcgga tattcaggaa accgtactga caaaaggact gaaaatcaac accggacctc
     9901 agcttgctgc caacatcacg cttgattatt tccatcccaa aatgtggttt gccgatgtaa
     9961 cgctcaacta tttcgacaat aattatctcg actttgcgcc caaccgattc acggcaagta
    10021 atgtggcaat gtatgaagca gatgctaccg gcgaagcaaa aagaatactg ggtactcagg
    10081 aaaaactcaa cgccggattt ctgctcgatg cctccatcgg aaaagtggtt tacctcccga
    10141 aacgccgttc gatgaatatt aacctcagtt tcagtaatat tctgaacaat acaaacatga
    10201 taacaggtgg attccagcaa ggcagaatgc cgatgctcga tgaaacaagt ctggacatgg
    10261 caaacctgaa cagattcccc aataaatact attatgcctg gggatttaat atgtttctga
    10321 accttggatt cagattttaa ttatgaaaac agtaaaaagt aacaatatga aaatcaaatc
    10381 attaatatat ataattgttc cggttgttct tttaacttcg tgcaacaaaa cggaatttga
    10441 tgctatcccg gtgagtgaac tgatgggtga ttcgctgaaa acaacctaca ccatcgaaca
    10501 actcaaaact gattttatga ccaacagcga tgcttactcc gatccggtag gcaagatggc
    10561 aggactctac acggccgata aaattaattc gtcgcaggat gttgttatca gtggatttgt
    10621 aacttctacc gacgtggaag ggaatgttta taaatatttt gtcgttcagg aagacaaacc
    10681 caacgggcag gctctcaaag tttcgatcga tgcatccggc ctttcggctg tttatccgct
    10741 gggtcagcag gtttggattc gctgcaacga cttatacatt ggtaaatacg gtgaagcacc
    10801 acagctgggt tcgctctata ttaacaagga acgcatgaaa atatccggtg ccgacaccat
    10861 ttacagaacc gaacccggaa gaatgcccct ttttacggct caaaagcatg tttactcata
    10921 cggaatgcct caaccagaca gaataaaagc cgacaccatg acggtggctc aaatcaaggc
    10981 agctgattac aaaaaactgg taaacaagct ggtatgtatc aaaaatgcgt atttcaccgg
    11041 attcgacgga aataaaaaag cactctcatc acaaaacatg atatttgctc cctctaccgg
    11101 cggagtaggt tatcctcagc tccgaaatat cagtgacgga accggcgatg tggccatcgc
    11161 taccagcgag tacgccaaat ttgccaccaa acctcttcca ccgtccaatt tcagtggtaa
    11221 tattacagtt attgtcggtt ggtataagaa ctacaccgat gcagcaggtg tatatcagct
    11281 cacgctgaga acactggatg atttgggtaa aggttttgaa acctatctgt cgaatattaa
    11341 ctacactaaa taaaacaacg taaaatctta tgaagaagaa attaatatat ggatttttgc
    11401 ttttgacggc ttcagtaatt actgcctgta caaatgcaac tgactacgat ctggaaaatc
    11461 ctgaatcgca aaaggttatc tttaccgaaa catttgaaag cggtaccctg ggcgatttta
    11521 ccacccaaag tgtaacaggt gatcaggttt gggtgggcga tgccagaggt tatgctttta
    11581 tgaccggtta tgtgagtgcc acacgcgaaa atatagccaa cgaagactgg ctgatttcgc
    11641 cgcaaatcaa tctgaccgat gttacgaccg ctcattttac ttttgaacat gtagcccgct
    11701 atttcgctga cctggttaac gaagctactg tatggatttc agataattac gtttcaggag
    11761 caccatccac agctacctgg actcaggtgg taacaagccc tttcagagat cccggagcat
    11821 gggattttca gccttcagga caacttagcc tcactgccta cgccggaaag aaaatacaca
    11881 ttgctttcaa gtatatttct acaaatacga aagccggttc gtgggaaata aaaaacatgg
    11941 tggttgcaac cggtgaagct gtcaatgaaa acgtaaacta tggtttggga acagctgctt
    12001 ctccttactc cgttctcgga ggcacctaca accagaataa tacctcagca tggctcaaag
    12061 ggacaatcgt cggatatgtc tggtatgtgg caggaaaagg aaattcattc tatttcagtg
    12121 gcgatacttg tactcaggcc accaacatac tggttgccga ctccggaagt ctgtatctga
    12181 gtcaggcaat ggctgttcag cttcccgcag gtgctgttcg aaccggaatc aacctgaaag
    12241 acaataaatc tttgtatggt aaaaaggtga cactctacgg tgatcttact gcctatttcg
    12301 gcattgccgg tctgaaaaac acatcttact acaagctgga agatggtaca acaggcggaa
    12361 acaaaccctt cgatccctct acggctattt actacgaaac ctttgccacc aatctgggca
    12421 aatttaccac tcaaaacgtt accggcggtg aaatatggac ttataatgga acttataagt
    12481 gtgcgtacat gaccggattt gtgagcagtc aaaacagagc caatgaagac tggctcattt
    12541 cgcccgagat cgatctgacc gggaaaagcg atattgcact ggcatttgat catgtgttcc
    12601 gctatggtgg agagccggct aacgaagcta ctgtttgggt ttctgaaaat tacagctcag
    12661 gcttgcctgc tacagctacc tggactcaga taacgaccaa ctttacgaat gcctccacct
    12721 gggattttta caatacaggc gacataagtc tgaaagccta tgcgggcaaa aaaatcaaga
    12781 ttgcacttgt gtacaaatct acctcaacca aagctggtac ctgggaagtg aaaaacttta
    12841 ttgtgaagta aaaaaaaaca atattttatc gttttagaca aaaaaaagga agctgtcgca
    12901 tgcgggcagc ttcttttttt ttaactgtaa tttcgaaatt cgaaaaatct attttcagaa
    12961 agttatcaat ccgcgtccgc tcatctcttt tgggatatca atacccaaaa cctggcaaac
    13021 agttggtgaa acatcagcca aaattccggg ttcgattgtt gcatttttat catttgtaac
    13081 aaaaacaaat ggcaccggat tgagagagtg agctgtattg ggcgaaccgt cggcattgat
    13141 tgcattgtcg gcatttccgt ggtcggcaat gataatggct tcgtaatcgt tagcctttgc
    13201 ggcctcaatc actttttcca cgcacacgtc aatcgtttca acggctttaa cgatggcttc
    13261 ttttacacct gtatgaccta ccatatcgcc gttggcaaaa ttcagcacga taaaatcaaa
    13321 tttctgagaa ttcagtgcat ccaccattct atcggcaatt tcaggcgcgc tcatctccgg
    13381 ttgtaaatcg taagttgcta cttttggaga aggaacaaga agacgctctt cacccgggaa
    13441 ttcagcctca cgaccgcctg agaagaagaa tgtaacgtga gcaaattttt cggtttcggc
    13501 cacccgcagt tgtttcaagc caagacccga aacgtattct cccatcgtat tctgtacatt
    13561 gtccttatca aaaagaacat gcaacccttt gaaagaactg tcgtacggcg tcatggtgaa
    13621 atattgcagc ggaatagtgt gcataccgtg ttcgggcata tcttcctgtg tcaaaacgat
    13681 tgtcaattct tttgcacggt cgttacggaa gttgaagaaa ataaccacat cgccttcttc
    13741 gatttttgca actggttttc catccgaatc cacatgtaca attggtttaa taaattcatc
    13801 tgtaacgcct tcatcataag aatcttgcat tgcctgtacc atatcagaag caggtgtacc
    13861 tactccatta accagtaaat catatgcctg ttttacacgt tcccagcgtt tgtcgcggtc
    13921 catagcgtag tagcgcccga taatagtagc gatttttccg gtggtttggt caatgtggtt
    13981 ttgaagttgt tcgataaatc ctttaccact cttcgggtct gtgtcacgcc cgtccataaa
    14041 agcatgtaca taagtgtctg taacgccgta ttcgttggca atatcaatta atttcattaa
    14101 atgatccagc gaactgtgaa cacctccgtc agaaaccagg cccagtaagt gaattttttt
    14161 tccgttatcg cgggcgtatg tatatgcttt tacgatttca gggttttgca tgatgctgtt
    14221 gtcacgacaa gcacggttaa ttttcactaa atcctggtaa accacacgtc cggccccgat
    14281 gttcaggtga cctacttccg aatttcccat ctgcccgtcc ggaaggccta cgttctcacc
    14341 cgaagcaagt aaataagaat tcggataagt agccaatagt ttatcccaat aaggcgtagg
    14401 agtgttgtaa atcaggtcag cttccgattg gttgccaatg ccccaaccat ctaaaatcat
    14461 taataaagct ttttttctca tattttttaa gttttgtttt atattctgtt ttcggggtgc
    14521 aaaattactc attttttggc acttatcctt gaaattcttc gaagattttg catgcttgca
    14581 gattgatttg tcatacaaga atgtcggacc tcctgaaagc aaaaacttca tgctccgtgc
    14641 ttccaacttc caacttaata aattatcttt gcatcctcaa aaacactatt gaatgtttat
    14701 tgtactcagt tttatgctgg cgggaattat agtcggttac ctgcttcgca gtaaaaaaat
    14761 ccgttttttt caaggactaa ttataacact tatatggctg ttattgtttt tacttggact
    14821 ggaaatcggt gctaataaag aggtcgtttc gcagtttggg aaacttggtc ttgaagcttt
    14881 catcattgct gccggaggaa ctctgggcag tgtgc
//
LOCUS       DAORKP010000039        14791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_320_length_14791_cov_9.503529, whole genome shotgun sequence.
ACCESSION   DAORKP010000039 DAORKP010000000
VERSION     DAORKP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14791
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_14791_cov_9.503529"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1158)
                     /locus_tag="PLN75_03645"
     CDS             complement(142..1158)
                     /locus_tag="PLN75_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRG03244.1"
                     /translation="MEAVKLEYTPKDFKSDQQVRWCPGCGDYAIINSLHKVMAEMGIP
                     PHEIAVVSGIGCSSRLPYYVSSYGFHTIHGRGAAVATGVKVANPALTVWQVTGDGDCL
                     AIGGNHFIHSVRRNIDINVLLFNNQIYGLTKGQFSPTSLKGQVSKSSPYGTIEEPFRP
                     AELTFGARGTFFGRLLDVDLKNAQQVMLAAAEHKGTSIIECLVNCVIFNDGAHNWLAE
                     KNTRPDKMVFLEHGKPMIYGVANDKGLILENGQLKAVTIGENGITEADILVHDARNPN
                     PTLQWELAMMKGPEMPVALGIIRDVPAESYNDAMDKQIADIKAKSKIKCFDDLVDSLE
                     QWEI"
     gene            complement(1165..3012)
                     /locus_tag="PLN75_03650"
     CDS             complement(1165..3012)
                     /locus_tag="PLN75_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRG03245.1"
                     /translation="MENINVEEMDNVVVLFAGDSGDGMQLTGTLFSNISAEIGNEIST
                     FPDYPAEIRAPQGTLGGVSGFQVHIGSGKIYTPGDEADVFVAMNPAALKINIRKVKKG
                     GTIIYDENSFQKNDLTKAGFATDNPFTELGVSETITLVPTPLTNLTQTSLEGFGLDNK
                     DILRSKNMFALGLICWLFNRPMDKAEHFIHNKFRKKPAVLEANLKVLNDGFNYGNNLH
                     LAISTYSIVRSDSTLPGKYTSISGNTATAYGLIAAAEKAGLQLFLGSYPITPASDIMQ
                     ELALRRDLGVKVMQCEDEIAGITTAIGASFAGCLAATNTSGPGLALKSEAIGLAVMAE
                     LPLVVIDVMRGGPSTGMPTKTEQTDLLQALHGRNGESPVVVIAPGTPDDCFHYTFMAS
                     KIALEYMTPVIVLTDAFLGNGTSLWRLPKLAELPEIKPNYVPADLTGYNATTRNEDTK
                     VRYWAVPGTEGFAYRNGGLEKDYYKGGISSDPENHQKMVETRAAKVKGVENMIPDLEI
                     FGNENADLLIVGWGSTRGHLMSSVVSMNKKGKNVAMVHFNYINPLPRNTEEVLKKFKK
                     VVVCELNSGQFAGYLRAEIPGLNILQYNKVQGQPFTVTELEKQFEIILK"
     gene            complement(3206..4603)
                     /gene="asnS"
                     /locus_tag="PLN75_03655"
     CDS             complement(3206..4603)
                     /gene="asnS"
                     /locus_tag="PLN75_03655"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRG03246.1"
                     /translation="MQRTKIVDALKSTAFGSEVNVKGWVRTRRGNKNVSFIAINDGST
                     IKNIQVVVDLSRFDEEYLKPITTGACISVTGSLVESQGQGQSVEIQADEVLIYGSADP
                     ETYPLQKKGHTLEFLREIAHLRPRTNTFGAIFRIRHQMAFAIHKFFHDRGFFYFHTPI
                     ITASDAEGAGQMFQVTTLDLENLTKTPEGKVDYSDDFFGRPANLTVSGQLEGELAAMA
                     LGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFYEIEENMQLAQEFIQYCVKWALDN
                     CQDDLNFLNEMYDKELIERLKFVVENDFIRLTYTEGIKILEQAVANGHKFEYRVGWGT
                     DLQSEHERYLVEQHFKKPVILTDYPQEIKAFYMKLNDDGKTVRAMDVLFPKIGEIIGG
                     SQREEDYEKLRTRAAGMGVPEKDIWWYLDTRRFGTAPHAGFGLGFERLLLFVTGMANI
                     RDVIPFPRTPNNADF"
     gene            complement(4805..6403)
                     /locus_tag="PLN75_03660"
     CDS             complement(4805..6403)
                     /locus_tag="PLN75_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRG03247.1"
                     /translation="MKLMNVVKLAGFVLAILLTTSCASRTGSAEGKARSKPPRNSTVS
                     TATGWTYENENFTGANGYASSGFAAHENYRPQIPAGMVVVEGGTFMMGEKTDMMTAQR
                     NNLTRRVSVGSFYMDQYEVSNLNWREYVHWMEVVFGATAPRLVDKARPNVDKSSDPMT
                     YNEPYLDRYYSHPAFNNYPVVGVTWEQAMDYCQWRTDRMNELVLVRAGVLAEPDFSYV
                     QKENNLDSITKNFVFNTDKYLLQRSYNPKPGSKPLKNAFGATRKADMGDGLMYPNFRL
                     PTEAEWEFAAYGLKAEKNGLLPEGRIYPWTGDQLRNSEKGKQQGQMQANYVRGRGDMM
                     GTAGDLNDKYTTTAPVNEYKPNDFGLYNMAGNVNEWVMDVYRSSSSQDVAEYNPFRGN
                     VDRSYLADFKDENGNYNLKIDSLGRVAYVVLPSEDLRGFKDGSNQLVTDFKLYTDPEG
                     ERALRNNTQLDPTDVLAPKITNKTRVYKGGSWKDRSYWLNPATRRYLDQDKSANDIGF
                     RCAMTMLGSINDQPVKKKTTVKKK"
     gene            6673..7530
                     /gene="folP"
                     /locus_tag="PLN75_03665"
     CDS             6673..7530
                     /gene="folP"
                     /locus_tag="PLN75_03665"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRG03248.1"
                     /translation="MSSIPFQLNLGGKLFDLSEPAIMAIVNVTPDSFYGLSRLFTEKD
                     VLNRVENALKEGAAMIDIGAYSTRPGARHISEAEEQERLREPLKSVRKHFPDAIISVD
                     TYRAGVARWAVTECNIQIINDISGGTLDDLMFETVADTGAAYILTHMRGTPQTMQQQT
                     VYENMLAEILEFFSKRIARLVHLGAKDIIIDPGFGFAKTLDQNYELLAKMSYFKQLEL
                     PILCGVSRKSMIYKLLDSSPEESLNGTTALNMLGLLNGADILRVHDVKEAAEAVKIFG
                     KYKSYQPQS"
     gene            7545..8333
                     /gene="cdaA"
                     /locus_tag="PLN75_03670"
     CDS             7545..8333
                     /gene="cdaA"
                     /locus_tag="PLN75_03670"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRG03249.1"
                     /translation="MAFNIGLKDIIDIILTAVLLYGTFKVLKRSGASTVFVGVMTFVI
                     VWFVVSQVLKMELLGGIFDAVVNVGAFALIVLFQTEIKRFFSRVGSSKNWGILNYFKK
                     FFKNTESEQTKTDYDLVQIVLACRNLSKTATGGIIVLTRENDLLFFTQTGETVDSRIS
                     SRLIENIFFKNSPLHDGALIISNRRLSAAACILPVSSNQNIPKRLGLRHRSALGITEQ
                     SDAIAIVISEETGHISWAVNGKLTINVKPEELEHFLSEEMKDVK"
     gene            8343..>9379
                     /locus_tag="PLN75_03675"
     CDS             8343..>9379
                     /locus_tag="PLN75_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HRG03250.1"
                     /translation="MIFSRKQILLIVLIPTISFCLTSPASAQNVHNIQVFEQMNIQFN
                     PGSIPDGLNETDGIIRLGNGRIAIKKISIPVFKRNTTATVKITLTSNGDPWDKSGSCF
                     IIPKTSAINMINIAAGKTSYPAVDTTKLEKLVGIIADSNYQPTVEIMRFMTPFGVGHF
                     SGTNDSVSKKRMPVYIDGWAKEVVWKQEVSDLIPLLSGEVYVGIYIDTWTKEGYNVSV
                     EIDFCESKIKQDKMPRRHVEPLINTVFYLGQSIPDIFARKNVSIPFSLPKKAKNICLK
                     YIVTGHGGHSGGDEFVQKENILSVDGNVVYRFVPWRNDCASFRRFNPSTGVWLQKRTV
                     AYISRNGRAEK"
     assembly_gap    9380..9479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9494..10762
                     /gene="purD"
                     /locus_tag="PLN75_03680"
     CDS             9494..10762
                     /gene="purD"
                     /locus_tag="PLN75_03680"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRG03251.1"
                     /translation="MKILLIGSGGREHALAWKIAQSPKLSKLFIAPGNPGTATIGTNL
                     NIHVSDFETVKQAVLSNQIDMVVVGPEDPLVNGIVDFFENDAALKAIPVIGPNKSAAR
                     LEGSKDFSKKFMEKHNIPTARYKSVSIANLADGIDFIKTLKPPYVLKADGLAAGKGVL
                     IINELNEAVNELKQMLDGKFGKASDTVVIEEFLSGIECSVFVITDGQNYQILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVNFADKIFMQKVEERIIKPTIEGLRSDGITYKGFVFFG
                     LIKVNDDPYVIEYNARMGDPETEVVMLRLKSDLVELLEGVANENLNEKTVEFDPRWAT
                     AVMLVSGGYPEDYTKGKIITGLEMVQGSIVFHAGTKETDGNIVTNGGRVIAVSSYGND
                     KNNALAACYKNAGIIGFEGKYFRRDIGFDL"
     gene            10917..12068
                     /locus_tag="PLN75_03685"
     CDS             10917..12068
                     /locus_tag="PLN75_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRG03252.1"
                     /translation="MAKILVIDDERSIRNTMKDILEFEKHKVVLAENGRVGLDTALND
                     EFDLIFSDIKMPEMDGMELLDQFLEKGLEVPVVMISGHGNISTAVECIKKGAFDFIEK
                     PIDLNRMLVTVRNALDKNTLVTETKTLKKKVASKNMMIGKSEAIERVREMIGKVAPTD
                     ARVMITGENGTGKEVVARLLYENSNRAQFPFVEVNCAAIPSELIESELFGHEKGSFTS
                     AHKQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRALQENEITRVGSDKVIKINVRVF
                     AATNKNLKAEIEKGNFREDLYHRLNVIPIHVPSLNERKEDIPLLVDFFTEQICTEQGI
                     QLKSFDSKAIKSLQERAWRGNIRELRNVVERLIILGNQKITKEDVERFS"
     gene            12147..12599
                     /gene="rpiB"
                     /locus_tag="PLN75_03690"
     CDS             12147..12599
                     /gene="rpiB"
                     /locus_tag="PLN75_03690"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRG03253.1"
                     /translation="MKPVTELRIAITNDHAGFELKKKIFDYLQNQSPLKLQDFGCQSA
                     ESCDYPDYAHPMATAIEKGLFDYGISICGTGNGISMVMNKHQGIRAALCWNEEIARLA
                     RLHNDANVLSLPARFITEKEALDMVDVFFSTGFEGGRHQRRIEKIPVK"
     gene            12629..13606
                     /locus_tag="PLN75_03695"
     CDS             12629..13606
                     /locus_tag="PLN75_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRG03254.1"
                     /translation="MPYTPPFHITEKIANLTAEIAEKVGSISATIGDIPHPKLRKENR
                     IKTIQSSLAIENNTLSIEQVTAIVEGKRVLGSPQEIQEVKNAFETYEHLLTFNPYSGQ
                     DLLMAHQILMKELVKDNGIYRSRSVGISDGNNIIHVAPPSDRVPYLVNDLLLWIENSE
                     LNPLIKSCVFHYEFEFIHPFSDGNGRMGRLWQTLILADWKQLFAWIPVESIVKQNQTE
                     YYQALQSADSNGDSTIFIEFMLKSILRAMEDLQKTQDVGVNVGVNVGVNENMILDLIK
                     NNSKITAKEITLHLNISLRQVQRLIASMKKNKLIFREGSDKSGEWRIIT"
     gene            13669..14691
                     /gene="thiL"
                     /locus_tag="PLN75_03700"
     CDS             13669..14691
                     /gene="thiL"
                     /locus_tag="PLN75_03700"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRG03255.1"
                     /translation="MTEISKYGEFGLIRHLTEKIKLQNPSSVKGVGDDAAVLDYKDKQ
                     VLVTTDLLLEGVHFDLIYTPLKHLGYKAAIVNFSDIYAMNGRPKQITVSLGVSKRFTV
                     ENLEDIYAGITLACENYGVDLVGGDTSASLTGLIISITCIGEAEKDKIVYRDGAGEND
                     LICVSGNLGSAYMGLQLLEREKLVFEGQQENVQPDFSGKEYILQRQLKPEARKDIIER
                     LAEKGIQPTAMMDISDGLSSELIHICSQSNVGCRVYEDKIPINMQAAVMAEELNMNIV
                     TAALNGGEDYELLFTCKLEDYEKIITLEDVGIVGHITKPELGMNLTGRNGEEIPLKAQ
                     GWNSLE"
BASE COUNT         4108 a         3212 c         3348 g         4023 t
ORIGIN      
        1 tttaaagaag tggccccctc taaatctccc ccttcggggg agatttaaag agtggattat
       61 agaatttttt atacagttgc aattcaaaag tcccccttcg agcctgtccc gtacttgttt
      121 cgggagggat ttaaggggat tttatatttc ccattgctcc agactatcca cgagatcatc
      181 gaagcatttg attttggatt ttgcttttat atcggctatt tgtttatcca tcgcatcgtt
      241 gtagctttcg gcaggaacat cgcgtatgat tcccagtgca acgggcattt ccggaccttt
      301 catcatggcc agttcccatt gcagtgtcgg atttgggttc cgggcatcgt gtaccaggat
      361 atcggcttcg gtaatgccgt tttcacctat tgttacggct ttcagctgtc cgttttcaag
      421 gataagtcct ttgtcgtttg ccactccgta aatcatcggt tttccgtgtt ccagaaatac
      481 cattttatcc ggtcgggtat tcttctcagc cagccagtta tgtgctccat cattgaaaat
      541 gacgcagtta accagacatt caataattga agtgccttta tgttctgcag ccgcaagcat
      601 cacctgctgt gcgtttttca gatccacatc gagcaaacgg ccgaagaatg ttccacgggc
      661 tccgaatgtg agttctgcgg gtcggaatgg ttcttcgata gtgccgtaag gagaagattt
      721 tgatacctgt cctttcagcg aagttggcga aaactgtccc tttgtgagac cgtaaatctg
      781 attattgaaa agcaatacgt tgatatcaat atttcttctc acactgtgaa tgaagtggtt
      841 acctccgatg gcaaggcagt cgccgtcgcc ggtaacctgc catactgtaa gtgccggatt
      901 agccactttc acgcctgtag ccacggcagc tccgcgaccg tgaatggtgt ggaatccgta
      961 actgctcacg tagtaaggca aacgcgagga acatccgata ccggaaacca cagcaatttc
     1021 atgcggagga atacccattt cggccatcac tttgtgcagg ctgttgataa tagcataatc
     1081 gccgcacccc gggcaccagc gcacttgttg atcactttta aagtctttgg gagtatattc
     1141 taattttaca gcttccatag cagtctattt taagatgatt tcgaattgtt tttcaagttc
     1201 cgttacggta aagggctgac cttgcacttt gttgtattgc aggatattca gaccgggaat
     1261 ttcagcgcgc aggtaaccgg caaactgccc ggaattgagt tcgcatacta ctactttttt
     1321 aaatttcttc agaacttctt cggtgtttcg cggcagggga ttaatgtaat tgaaatgaac
     1381 cattgccaca tttttacctt ttttattcat agaaacaact gaactcatca ggtgtccgcg
     1441 cgtacttccc catccaacaa tgagtaaatc agcattttcg ttaccgaaga tttccagatc
     1501 gggaatcatg ttttccactc cttttacctt ggcggcccgt gtttccacca tcttctgatg
     1561 gttttcggga tctgaggaaa ttcccccttt gtagtagtct ttttccaatc cgccgttgcg
     1621 gtatgcaaat ccttctgttc cgggtacagc ccagtaacgc actttggtgt cttcattgcg
     1681 cgtagtggca ttgtagcctg tcaggtcagc cggtacgtag ttgggtttga tttcaggcaa
     1741 ttcagccagt ttgggcaacc tccagagtga agtaccgttt cctaaaaatg catcggtaag
     1801 cacgataacg ggtgtcatat attccagggc aattttggac gccatgaagg tatagtgaaa
     1861 gcaatcgtcg ggtgttccgg gagcaatcac caccacgggg ctttcaccgt tccgtccgtg
     1921 taatgcctga agcaggtcgg tttgttccgt ttttgtcggc atacccgtag aagggccgcc
     1981 acgcattaca tcaatcacca cgagtggtaa ttcagccatt acagccagtc cgattgcttc
     2041 ggacttcagc gccaggccgg ggcctgatgt attcgtagct gccagacatc cggcaaatga
     2101 agcgccaatg gctgtggtaa tacctgcaat ctcatcttcg cactgcatca ctttcactcc
     2161 cagatcccgg cgtagcgcca gttcctgcat aatatcagaa gcaggagtaa tgggatagct
     2221 gcccaggaaa agctgcaatc cggctttttc ggcagctgca atcagtccgt aggctgtagc
     2281 cgtatttccc gaaatactgg tgtattttcc cgggagcgta gagtcagaac ggacaatcga
     2341 gtaagttgag attgctaaat gcaggttgtt tccgtagtta aagccgtcat tcagcacttt
     2401 gagattggct tcgagtacgg ccggtttttt tctgaattta ttatgaatga aatgttcggc
     2461 tttatccatc gggcggttaa ataaccagca aatcagacca agagcaaaca tgtttttact
     2521 tctcaggata tccttattgt ccagtccgaa accttcaaga cttgtctggg tgaggttggt
     2581 tagcggagtt ggcactaagg ttattgtttc agagacaccc agttcagtga acggattatc
     2641 ggtggcgaaa ccggcttttg tcaggtcatt tttttgaaag ctgttttcgt cataaataat
     2701 tgttcctccc tttttcactt tccggatgtt aattttcagt gctgcggggt tcatagctac
     2761 gaatacgtct gcttcgtcgc ccggagtata tatttttccg gagccaatat gcacctgaaa
     2821 accggaaact cctcccagtg taccttgcgg agcgcgaatt tcggcaggat aatcggggaa
     2881 tgttgaaatc tcattaccga tttcggcaga aatgtttgaa aacagtgtgc ctgtaagctg
     2941 cataccgtca ccggaatcac ctgcaaacaa tacaacgaca ttgtccattt cttcaacgtt
     3001 aatgttttcc atataactat tttttttcga ttttagataa attcaaagtg ataaaatcct
     3061 ttattcttaa ataattgcaa attattatat taaaattgtc ggacattctt gtctggcttc
     3121 tttattgttc aaaaaattta ctactcagat ttttttatat tccggtaatt cgcgttaatt
     3181 tgcctaaatt attgtaattc gtatgttaga aatccgcatt atttggcgtg cgggggaacg
     3241 gaatcacgtc gcggatattg gccattccgg tgacgaacag cagcagacgc tcaaatccaa
     3301 gtccgaatcc ggcatgcggt gcggtaccga accggcgggt atccagatac caccagatat
     3361 ccttttcggg cactcccatt ccggctgcac gggttctgag tttttcgtaa tcttcctcgc
     3421 gttgcgatcc gccgatgatt tcaccaattt tcgggaagag cacatccatg gcacgaacgg
     3481 tttttccgtc gtcgttcagt ttcatgtaga aggcttttat ttcctgcgga taatcggtca
     3541 ggatgaccgg tttcttgaaa tgctgttcaa ccagatagcg ctcgtgttcg gattggaggt
     3601 ctgttcccca tcctacacgg tattcaaatt tatgaccgtt ggcaacggct tgttccagga
     3661 ttttaatccc ttcggtgtaa gtgagtcgta tgaagtcatt ttccaccaca aatttcaatc
     3721 gctcaatcag ttctttatca tacatttcgt tcaggaaatt cagatcgtcc tgacagttat
     3781 ccaatgccca cttcacgcaa tactggataa attcctgtgc cagctgcatg ttttcctcta
     3841 tttcgtagaa agccacttcc ggctcaatca tccagaactc ggataaatgg cgtggtgtgt
     3901 tggagttttc agcccggaac gttggtccga atgtgtaaat tgccccgagt gccatagcgg
     3961 ccagttcgcc ttcgagctgt ccggaaacgg tcagattggc cgggcgtccg aaaaagtcgt
     4021 cggaataatc cacttttcct tcgggggttt tagtcaggtt ttccaggtcc agtgtggtca
     4081 cctgaaacat ttgtccggca ccctcggcat ccgaagctgt gataatcggg gtgtgaaaat
     4141 agaagaagcc gcgatcatgg aaaaatttgt gaatggcgaa tgccatctga tggcgaatgc
     4201 ggaagatggc tccgaatgta ttggttcgtg gacgcaggtg tgctatttcg cgaagaaact
     4261 cgagcgtgtg tcctttcttc tgaagggggt aggtttcagg atcggccgat ccgtaaatca
     4321 gcacttcgtc agcctgaatt tccacacttt gcccctgccc ctgcgactcc accagacttc
     4381 cggtaacact gatacaggct cctgtggtga tgggtttgag atattcttcg tcaaaacggc
     4441 ttaaatcaac tacaacctga atgtttttta ttgtggagcc atcatttatg gctataaaac
     4501 ttacgttttt gtttccccgg cgggtacgaa cccacccttt gacatttact tctgatccaa
     4561 acgctgtact cttcagcgca tcaacaattt ttgttctttg cataaaaaaa taaatctttg
     4621 atataatgtg ttttgaactg caaaattaat acttttaaat cattaaacta aatttattcg
     4681 gaataaaaaa acacctgctt tagaaacagg cgctttatta acatattttt gcgatttgac
     4741 aaaatgtcat cttttctaat atgaaaattg cagttaattc aaattaaaac tttcaattag
     4801 tttgttattt cttctttacc gtagtttttt tcttcaccgg ttgatcgttg atacttccga
     4861 gcatggtcat ggcacagcgg aatccgatgt cgttggccga tttatcctga tcgaggtaac
     4921 gcctcgtagc cggattgagc cagtacgaac ggtctttcca cgatccgcct ttgtaaacac
     4981 gggttttgtt cgtgatttta ggcgccagca catcggtggg atccagttgt gtgttattcc
     5041 ggagtgccct ttcgccttca ggatcggtgt aaagcttgaa atctgtaacc agttgattcg
     5101 atccgtcttt gaatccgcgt aaatcctcgc tgggcagcac tacgtaggct actcttccga
     5161 gcgaatcgat tttcaggtta taatttccgt tttcatcctt gaaatcagcc aggtacgagc
     5221 ggtcaacgtt tccacggaaa ggattatatt cggctacatc ctgcgaagat gaagagcgat
     5281 atacatccat tacccattcg tttacatttc cggccatgtt gtacagccca aaatcattgg
     5341 gtttgtattc gtttacaggt gcagttgtag tgtacttgtc attcaggtcg ccggcagtgc
     5401 ccatcatatc gccccgtcct ctcacgtagt ttgcctgcat ttgtccctgt tgctttcctt
     5461 tttctgagtt acgcagctga tcacccgtcc agggataaat ccggccttct ggcagcaatc
     5521 cgtttttttc ggctttcagg ccataagctg caaattccca ttcggcttca gtaggcagcc
     5581 tgaaattcgg atacatcagt ccgtcgccca tatcggcttt tcttgtagct ccaaatgcat
     5641 ttttgagcgg tttggatccg ggtttcggat tgtaactgcg ttgcaacagg tatttatccg
     5701 tattgaaaac aaagtttttt gtgatcgaat ccaggttgtt ttctttctga acataagaga
     5761 aatcgggctc agccagcact ccggctctta cgaggacgag ttcgttcatt ctgtcggttc
     5821 gccactggca gtaatccatg gcctgttccc aggttacccc taccaccgga tagttgttga
     5881 aagccggatg tgaataatag cgatccagat aaggttcgtt gtaggtcatc ggatcacttg
     5941 atttatccac attgggtctt gccttatcta ccagacgcgg agcggtagca ccgaatacca
     6001 cctccatcca gtgaacgtat tcccgccagt tcagattgct cacctcatac tgatccatgt
     6061 agaaagagcc taccgacacc ctgcgggtga ggttattgcg ctgagcggtc atcatgtctg
     6121 ttttttcgcc catcatgaag gtaccaccct ccactaccac cattccggcg ggaatttgcg
     6181 gtcgataatt ttcatgggct gcaaatcccg aagaggcata tccgttggct ccggtgaaat
     6241 tttcgttttc ataggtccat ccggtagctg tagatacagt cgaatttctc ggaggttttg
     6301 atcttgcttt tccttcggcc gaaccggttc gggatgcaca ggaggttgta agcaaaatag
     6361 ctaaaacaaa accagccagt ttaacaacgt tcattaattt catacaatgg ctatgataaa
     6421 taataagtta ttttttatga aacagatgca tcatttctcc ccgttttttt atcggaaagt
     6481 aattctgacg tttacagtca gttgtttttg aatgcttata aataactcaa attaaacaga
     6541 aaacgtatat tttattataa aatttttaat aattctacaa aaacagagcg aaaaagcgtt
     6601 tattttctta cttttgtaga gcataattct aatgtgaaac gcatttttat caaacatatt
     6661 ttgtgatttc taatgagttc catccctttt cagttaaatt tgggcggtaa attgtttgat
     6721 ttgtcagaac cggcaattat ggccattgtc aatgtgactc cggattcatt ttacggtttg
     6781 agcagactat ttaccgaaaa ggatgtgctg aaccgcgtag aaaatgcact gaaagaggga
     6841 gctgccatga ttgatatcgg ggcctattcc acccgtccgg gagcaagaca catttccgaa
     6901 gcggaagaac aggaaaggtt gcgtgagccc ctgaaatcgg tcaggaaaca ttttccggat
     6961 gccatcattt cggtagatac ttacagagcc ggcgtggcac gctgggcggt aaccgaatgt
     7021 aatatacaga ttattaatga cataagcggt ggaacgctgg acgatttaat gtttgaaaca
     7081 gtagccgata cgggtgctgc gtacatcctg acccacatgc gcggcacacc tcaaaccatg
     7141 caacaacaga ccgtgtacga aaatatgctt gccgagatac tggaattttt ctcaaaaaga
     7201 atagccagac ttgtacatct gggtgctaag gacataatca tcgatccggg atttggattt
     7261 gcaaaaacgc tggatcagaa ttatgagctg cttgccaaaa tgagctattt caaacaactg
     7321 gaattaccca ttctctgtgg tgtctcgaga aaatcgatga tttacaaact cctggattcc
     7381 agccccgaag aatcgctcaa cggcactacg gcactgaata tgctgggtct gctgaacgga
     7441 gcagatatcc tgcgcgttca cgatgtgaaa gaggctgcag aagccgtaaa gattttcgga
     7501 aaatacaaat cgtatcaacc tcaatcataa accctgaata gctgatggca tttaatatcg
     7561 gattgaaaga cataatagat atcatcctga cggctgttct gttgtacggc acattcaagg
     7621 ttttgaagcg ctcgggtgct tccactgtat ttgtcggggt gatgaccttt gtaatcgttt
     7681 ggttcgttgt ttcgcaggtg ctcaagatgg aactgcttgg cggtattttc gatgcggtag
     7741 tcaatgtggg tgcatttgcg cttatcgtgc ttttccaaac ggagattaag cgtttctttt
     7801 cgcgtgtggg atccagcaaa aactggggca tcctgaatta tttcaaaaaa ttcttcaaaa
     7861 acaccgaaag tgagcaaacc aaaaccgatt atgacctggt tcagatagtg ctcgcctgcc
     7921 gcaatctatc gaaaaccgcc acaggtggaa ttatcgtgct tacgcgcgaa aacgacctgc
     7981 tgtttttcac ccagacaggc gaaaccgtgg attcacgaat cagttcgcgg ctgatcgaaa
     8041 atattttttt caaaaacagt ccgctccacg acggagcatt aatcatttcc aaccgcagac
     8101 tatctgctgc agcctgcatt ctgcctgttt cgagcaacca gaacataccc aaaagactcg
     8161 gattaagaca ccggtcagcg ctggggatta ccgaacagtc ggatgctatc gccattgtga
     8221 tttctgaaga aaccggccat atctcttggg ctgtaaacgg aaaactaacc atcaatgtaa
     8281 aacccgaaga actggaacat ttcctctccg aagaaatgaa agatgttaaa tagctgacta
     8341 tcatgatatt ttcaagaaaa caaatcctgc tgatcgttct tatacctact atctcatttt
     8401 gcctgacttc acctgcatca gcacaaaatg tgcacaacat tcaggtgttt gagcagatga
     8461 atattcagtt caatcccggc tcaattcccg atggactcaa tgaaactgat ggtataatcc
     8521 gcctggggaa cggacggata gccattaaaa aaatcagtat tccggttttc aaacgcaata
     8581 ctaccgccac tgtcaaaatc accctcactt caaacggcga tccgtgggac aaatccggtt
     8641 cttgcttcat tattccgaaa acttcagcta tcaacatgat aaatattgct gccggaaaaa
     8701 caagttatcc tgcagtggac accacgaagc tggaaaaact cgtgggaata atagccgata
     8761 gcaattatca gcccacggtg gaaatcatgc ggtttatgac tccgttcgga gtgggacatt
     8821 tcagcggaac gaacgactca gtttcaaaaa aaagaatgcc ggtttatatc gacggatggg
     8881 caaaagaagt ggtgtggaaa caggaagtga gtgatttaat accgctgctt tcgggcgaag
     8941 tttacgtagg aatttacatc gatacatgga caaaggaagg atacaacgta agcgtggaaa
     9001 tcgatttctg tgaaagcaaa atcaagcagg acaaaatgcc caggagacat gtggagcccc
     9061 ttatcaacac ggttttctat ctcggtcagt ctattcccga tatttttgcc cgaaaaaacg
     9121 tgtcaatccc tttttcactg cccaaaaaag ctaaaaatat ctgtttgaaa tacatcgtaa
     9181 ccggtcatgg cggacattcc ggcggtgatg aatttgtgca gaaggaaaat atcctctcgg
     9241 tggatggcaa cgtggtgtac cgcttcgtac cctggagaaa cgactgtgcc tcattccgca
     9301 ggttcaatcc atctaccggt gtgtggctgc agaaaagaac ggtagcttat atttcacgta
     9361 acggacgtgc cgaaaaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     9481 aactaaattg acaatgaaaa tcttacttat cggatcggga ggacgggagc atgcgttggc
     9541 gtggaaaata gctcaaagcc cgaaactcag caaactattc attgctccgg gaaatccggg
     9601 cacagcaact ataggtacca atctcaacat ccatgtttcc gactttgaga ctgtaaagca
     9661 ggctgtgctc tcaaatcaga ttgatatggt tgtggtagga cccgaagatc cgctggtaaa
     9721 cggaatcgtt gatttttttg aaaatgatgc cgcactgaaa gcaattcccg taataggtcc
     9781 aaacaaatcg gctgcccggc tggaaggtag caaggatttt tccaaaaagt ttatggaaaa
     9841 acacaacatc cccacagcca ggtataaaag tgtttcgatt gcgaatctgg cagatggaat
     9901 cgatttcata aaaaccctga aaccacctta tgtactaaaa gccgacggac tggctgccgg
     9961 aaagggggtt ctgattatca atgagctaaa cgaggctgta aacgaactaa aacaaatgct
    10021 ggacggcaaa ttcggcaaag caagtgatac ggtggtcatc gaagaatttc tttcgggaat
    10081 agaatgttct gttttcgtga ttaccgatgg ccaaaactac cagatcctgc cagaagccaa
    10141 ggattacaaa cggattggcg aaggtgatac aggcctcaat accggcggaa tgggcgccgt
    10201 gtcgccggta aactttgccg acaaaatttt catgcaaaaa gtagaggagc gaatcatcaa
    10261 acctaccatt gaaggacttc gaagcgacgg cattacttac aaaggttttg tgtttttcgg
    10321 cttgataaaa gtgaacgatg atccatacgt gatcgaatac aatgcccgga tgggcgatcc
    10381 cgaaaccgaa gtggtgatgc tgcggctcaa atctgatctg gtggaactgc tcgaaggggt
    10441 ggcaaatgaa aacctcaatg aaaaaacggt agaattcgac ccacggtggg caactgccgt
    10501 aatgctcgtt tcgggtgggt atcccgaaga ttacacgaaa ggaaaaatca ttacaggact
    10561 ggaaatggtg cagggcagca tcgtatttca tgccggcacc aaagaaacgg acggaaacat
    10621 cgtaacaaac ggtggacggg tcattgccgt cagttcgtat ggaaatgata aaaacaatgc
    10681 cctggctgct tgttacaaaa atgccggaat tatcggtttt gaagggaaat atttcaggag
    10741 agatattgga tttgatttgt gaaatacaaa ttacttaaat tacgaattat gcgaattaga
    10801 gcgaatttcg cgaatttgga tgagtgctgt tttgaaaaaa gagataaaat taaatacatt
    10861 tttttagatt gtaacattta aaatgtctcc ttcgggggat tcagggataa attcttatgg
    10921 caaaaatact ggtgatagac gacgaacgaa gcatccggaa tacgatgaaa gacattctgg
    10981 aattcgaaaa acataaggtt gtactggccg aaaacggtcg cgtgggattg gatacagcac
    11041 tcaacgatga atttgacctc attttttcgg acatcaaaat gccggaaatg gacggcatgg
    11101 aactcctcga ccagttcctt gaaaagggat tggaagttcc tgtggtgatg atttcagggc
    11161 atggaaatat cagcacagca gtagaatgta tcaaaaaagg cgcctttgac tttatcgaaa
    11221 aacccatcga cctcaaccgc atgctggtaa ccgtgcgcaa cgcgctggat aaaaacacgc
    11281 tggtgaccga aaccaaaacg ctgaaaaaga aagtggcttc caaaaacatg atgattggga
    11341 aatcagaagc catcgagcgg gtacgtgaaa tgataggtaa agtggctcca accgatgccc
    11401 gggtaatgat tacgggcgaa aacggcacag ggaaagaagt ggtggcacgc ctgctttatg
    11461 aaaacagcaa ccgcgcacaa ttcccattcg tggaggtaaa ctgtgctgcc ataccttcgg
    11521 agctgattga aagcgaactt ttcggacacg aaaaaggctc gttcacttcg gctcacaaac
    11581 aacggatcgg taaattcgaa caagccgaca acgggaccat ttttctcgat gaaataggcg
    11641 atatgagcct ctcggcacaa accaaggtgc ttcgggcgct gcaggagaat gaaattacac
    11701 gggtgggtag cgataaggta ataaaaatca acgtgcgggt ttttgcagct accaacaaga
    11761 atctgaaagc ggaaatcgag aaaggcaatt tccgcgaaga cctctaccac cgcctcaatg
    11821 tcatcccaat ccacgtgcca tcgctcaatg agcgcaagga agacattccg ctgcttgtcg
    11881 attttttcac ggagcagatt tgcaccgagc agggcattca gctgaaatcg tttgattcaa
    11941 aagccattaa atcgcttcag gaacgcgcct ggcgaggtaa tatccgcgaa ttgcgcaacg
    12001 tggttgaacg gctcatcatt ctgggcaacc agaaaattac gaaagaagat gtagaaagat
    12061 tctcgtaaca ggccctgcca attccttcaa ataaaaaggc ttaggaaaaa tcgttaaaat
    12121 attacacaaa aaaaaacaca gctcccatga aaccagttac agaacttaga atagccatta
    12181 ccaacgacca tgccggtttt gagttgaaaa aaaagatatt tgactacctg caaaatcaat
    12241 cgccgctcaa attacaggat ttcggctgtc aatcggccga aagctgcgac taccctgatt
    12301 acgcccatcc gatggctaca gctatcgaaa aaggattatt tgattacgga atttcaattt
    12361 gcggaacggg aaacggcatc agcatggtga tgaacaaaca tcagggaata cgcgccgcgc
    12421 tctgctggaa cgaagaaatt gcccgccttg cccgcctgca caacgatgcc aatgtacttt
    12481 cacttcctgc acggtttatt accgaaaaag aggcgctgga tatggtcgat gtttttttct
    12541 ccaccgggtt cgaaggagga aggcatcagc ggagaattga gaagattcct gtaaaataat
    12601 ttataatcag cagtttatag tttgcagaat gccttatact ccaccttttc atatcacgga
    12661 aaaaattgct aatctgactg ctgaaatagc tgaaaaggtt ggatcaatca gtgcgacaat
    12721 tggtgatata ccgcatccga agttgaggaa agaaaaccgc attaaaacca ttcaatcatc
    12781 attagccatt gaaaataaca cactcagcat tgagcaggtt accgccattg tagaagggaa
    12841 gcgtgtgctg ggttcgccgc aggaaattca ggaggtgaaa aatgcgtttg aaacttatga
    12901 acatttattg acatttaatc cttattccgg gcaggatttg ttgatggcac atcaaatact
    12961 gatgaaagaa ctggtgaaag acaacggaat atatcggagc aggagtgtcg gtatttcgga
    13021 tggaaataat ataattcacg ttgcaccgcc ttccgatcgc gtaccttatt tagtgaatga
    13081 tttgcttctg tggatagaaa acagcgaatt aaatccattg attaaaagtt gtgtgtttca
    13141 ttacgaattt gagtttattc atccattttc cgatggaaac ggcagaatgg gacgtctctg
    13201 gcaaacgctt attcttgctg attggaaaca gcttttcgcc tggattccgg tagaaagtat
    13261 tgtaaagcaa aatcaaacag aatattatca ggcattacag tcggcagaca gcaatggaga
    13321 cagcactatt tttattgaat ttatgctgaa aagtattctt cgggctatgg aagacttaca
    13381 aaaaacacag gatgtcggtg taaatgtcgg tgtaaatgtc ggtgtaaatg aaaatatgat
    13441 tttggattta attaaaaaca attcaaaaat aacagctaaa gaaataactt tgcatctgaa
    13501 tatttcactt cgtcaggtac aaagattgat cgcttcaatg aagaaaaaca aattgatttt
    13561 ccgcgaaggt tctgataaat caggtgaatg gagaataatc acataaacaa aaacatattt
    13621 aacaactaac aattattttc ttccttcggg gagtttaggg gctaacttat gacagaaata
    13681 tcaaaatacg gtgaattcgg gttaatccgt cacctgaccg aaaaaataaa actgcagaat
    13741 ccgtcatcag tcaaaggcgt gggcgacgat gctgccgtgc tggattacaa ggataaacaa
    13801 gtgcttgtta ccaccgatct gctgctggaa ggggttcatt ttgatttgat ttatactcct
    13861 ttgaaacacc tgggttacaa agctgctatt gtcaattttt ccgacattta tgccatgaat
    13921 ggccggccaa aacaaatcac ggtttcgctg ggtgtttcca aacgattcac ggtggaaaat
    13981 ctggaagata tatatgccgg aattacactg gcctgcgaaa attatggagt ggacctggtg
    14041 ggaggcgaca catcagcatc gctaacaggt ctcattatca gcattacatg cattggtgaa
    14101 gccgaaaaag acaaaatcgt gtatcgcgac ggagccggag aaaacgacct gatctgtgtt
    14161 tcgggaaatc ttggatctgc ttatatggga ttgcagctgc tcgaacgcga gaaacttgtt
    14221 ttcgaaggtc agcaggaaaa cgtccagccc gatttcagcg gaaaagagta catcctgcaa
    14281 cgccagctca agcccgaagc ccgcaaggac atcatcgaaa ggctcgctga aaaaggcatc
    14341 cagcctacgg caatgatgga tatttctgac ggattatcgt cggagttgat tcatatctgc
    14401 tcacaaagta atgtaggctg ccgtgtgtac gaagataaaa ttccgattaa tatgcaggca
    14461 gctgtgatgg ccgaagaact gaatatgaac atcgtcacgg cggcactcaa cggcggcgaa
    14521 gactacgaac tgcttttcac ctgcaaactc gaagattacg aaaaaatcat tacactcgaa
    14581 gacgtgggta tcgtgggaca tatcaccaaa ccggaactgg gtatgaatct gacaggacga
    14641 aacggcgagg aaattccgct gaaagcacag ggatggaatt cactggaata gctccctgaa
    14701 cgtttttagt gaacaacaaa tatcttatca tttgaccatg aaaaaaactc acattcttca
    14761 actgctgatg gtttctctgc ttttttgcat a
//
LOCUS       DAORKP010000040        14742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_325_length_14742_cov_9.370396, whole genome shotgun sequence.
ACCESSION   DAORKP010000040 DAORKP010000000
VERSION     DAORKP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14742
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_325_length_14742_cov_9.370396"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PLN75_03705"
     CDS             <1..1511
                     /locus_tag="PLN75_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRG03256.1"
                     /translation="INSCNSYFMKIFTTSQIRQLDEYTIEHEPIASIGLMERAADRLL
                     QQFKKDWSMNRDVLILAGPGNNGGDGLALGRMLLQIGYEVQVILIHGGKISPDCKQNK
                     DRLEQYFPDFFTEQINIFSPPEITKKTIIVDALFGSGLSKPLENTYAAVVDWINRSGN
                     TVVAIDIPSGLCGEQCAADADPVVKANLTYTLQFPKTAFFFAENEKYTGKWKVIDIQL
                     HPGAVAKTETNIYLTEKADIRTMLKPRERFSHKGTFGHTLIWAGKKGMAGAVVLSAKA
                     ALRSGAGLVSVHSVEDNRVILQSTVPEAIFVSELPDLTNYNSIAIGPGLGTDFQTTEM
                     LFEMLKTMYKPCVLDADALNIISRHKNFLEFVPENSILTPHPKEFDRLFGASTHSQER
                     MEKAITAAREHGLVIVLKGANTFISTPAGKLYINFTGNPGMAVGGMGDALTGIIAGML
                     AQGYSPVESSISGVYLHGLAGDLALSSQSEESLLPSDLIQNLGNAFTTIRKS"
     gene            1727..2995
                     /locus_tag="PLN75_03710"
     CDS             1727..2995
                     /locus_tag="PLN75_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRG03257.1"
                     /translation="MKRLLFFIVIFLTILPVRSQEITLAACQEQARANFPLTRQQGLI
                     DQVTKNTIEALMAQYFPQFRLSGQASYQSDVTKIDLNMPDGFPKIDIPTPEKDQYKLY
                     LDVSQTIFGGGSLGVQQQFTKAEAEVNKQRIEVELYKLRDKINQVYFGILLADEQQKQ
                     TDILKNDLNTALEKVKVMVKNGVSLETNQFNLEAELITINQNIKNIELGRSALLEILG
                     KLIGKDLDKETQLTKPGTIRLKSDFVRPELTLIDLQKNTLSLQNLQLAAQNVPEISLF
                     LQGGWGKPALNLFSTKFEPYYLGGVRLQWNISRLYTILRERRIIETRLKMYDVEKDMF
                     TMNNDIEQTQQLSKINQLNLSLKSDDELIALRSKIKQTSQVQLENGVITSADFIRDAN
                     AENQARQKKALHEMQLILEQYHLLWMKATP"
     gene            3160..4074
                     /locus_tag="PLN75_03715"
     CDS             3160..4074
                     /locus_tag="PLN75_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRG03258.1"
                     /translation="MAGVLFIVLLLTSCKSTTNEAFASGYFEAVETIVSARGNGTIQY
                     LNVEEGMDLQADTKVGQIDSTQIYLTIRQIQAQKGAVLSRKPDISSQTAILKEQLAHA
                     NRELIRVENLNAGGAATQKQLDDARSAVAVLNRQLSAQQTTLDKTSASVGKETNPLEV
                     QISLLQDQLEKCRIINPVTGTVLVKYAEPTEMTAIGRPLYKIADLSTLQLRAYITGKQ
                     LGSLKLGQKVKVTTDLGKEKPAEYEGTVNWISNKAEFTPKTIQTHEERSNEVFAIKVA
                     VKNDGFLKIGMYGEIAPPNLPKGEAIKE"
     gene            4149..5129
                     /locus_tag="PLN75_03720"
     CDS             4149..5129
                     /locus_tag="PLN75_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG03259.1"
                     /translation="MPLFQTNTQSHEPASPLRGVGRLYISVSNLSKTYENGKVRALDD
                     VSFTVDEGEIFGLIGPDGAGKTSIFRILTTLLIPDSGKASVLGYDVVKDFRKIRNEIG
                     YMPGRFSLYPDLSVAENLNFYARLFGTTIDENYHLIRDIYVQIEPFKKRKAGQLSGGM
                     KQKLALCCALIHKPKVLFLDEPTTGIDPVSRKELWEMLRKLKDEGICIFVSTPYMDEA
                     VRCDRIALIQQGVILKTETPEAIVKQYDKPIFAVGADNTYQLLKVLEQYPNRLNSYAF
                     GEFAHLVTNGNEDELSDFLEKKGLKDITINRIKASIEDCFIDLSIRNFST"
     gene            5253..5987
                     /locus_tag="PLN75_03725"
     CDS             5253..5987
                     /locus_tag="PLN75_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG03260.1"
                     /translation="MYSITAEKLTKKFGNFTAVDAITFEVKEGEIFGFLGANGAGKTT
                     AMRILCGLSYPTSGKASVAGFDVYKQQEDIKKNIGYMSQKFSLYENLTVSENIEFYGG
                     VYGLRMREIRPKTKNILQKLGLDSEANKLVSSLPLGWKQKLAFSTAIFHEPRIVFLDE
                     PTSGVDPVTRRQFWEMIYEAASRGITVFVTTHYMDEAEYCNRISLMVDGRIDALDAPK
                     NLKAEHNAQSMDEVFYALARKAKRSD"
     gene            6068..7228
                     /locus_tag="PLN75_03730"
     CDS             6068..7228
                     /locus_tag="PLN75_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG03261.1"
                     /translation="MINKRKYKQSHSSTPPSGGWGAILMAFLHKEFLHVFRDKRTLLV
                     MFGLPIAQILIFGFALTNEVKNARLLIVSPVQDTESKKLIEKIDASSYFTVTHIVPTR
                     QNLEHYFRNNEAQCALILPDDFSASGVSPSQVQIVADATDPNFAKTVVFYLSAILGEF
                     QQQSQPAQSIPMAIKTEVRMLYNPSLMGAMNFVPGVIALIMMIVCTTLTSISIVREKE
                     TGTMETLLVSPLKPFLLIISKAIPYTVLSLVNLSFILLLSNLVLDVPIRGSILLLLFE
                     CLLYIIACLALGLMISNIASSQAVAMMISMAGILLPTLLLTGFLFPLENMPWIFQWLS
                     NVVPARWFFIIVKSIMLKGVGLEDVWLETTVLIGMTVFLLAVSLKRFKIRLE"
     gene            7225..>8312
                     /locus_tag="PLN75_03735"
     CDS             7225..>8312
                     /locus_tag="PLN75_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG03262.1"
                     /translation="MRILLFILRKEFIQIFRDRMMLPMLLLMPLMQLLILPFAADMDV
                     RHLKIIIVDNDHSPVSKSLISKFTASDLFEIYAISGSYNEALKLIETDKADIILQIPH
                     GFERNLIREGSEKVAITADAINGVKAGLGTSYLNAIIQNFNQDIALEWSNATSLTPPL
                     DVRYSFWYNPYLRYKNFIVPAILVLLVTVISGFVSALNIVSEKEKGTIEQINVTPIKK
                     WEFIIGKVLPFWLIGIFDFSLGLLIMYYVYGIQIVGSLSTLYLFAIVYITGILGFGLL
                     ISTVSHTQQQSMFVAFFFIMIFILMSGMFTNVESMPPWAHTISSLIPVTHFMHAVRAI
                     VLKGSVFLDLWVDMVYILCFDVVAIAAAV"
     assembly_gap    8313..8412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8413..8899)
                     /locus_tag="PLN75_03740"
     CDS             complement(<8413..8899)
                     /locus_tag="PLN75_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRG03263.1"
                     /translation="MYFSKKPVYLLLMSIIAILATAQTPAVSTIVEKEGLYFTDATQT
                     KLYTGDYREYYDNGSLKLEMHIKNGSPEGTYVVYFENNKPKEIRSYKNGQFHGLWRSY
                     SAAGQLISEAEYKNDKKHGVWRIWDENGTKRYEMNYNDGKKVGVWRMWNENGELTGEK
                     KY"
     gene            complement(9165..10304)
                     /locus_tag="PLN75_03745"
     CDS             complement(9165..10304)
                     /locus_tag="PLN75_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03264.1"
                     /translation="MKKFILSLMAITVVMMSLTFNSCKPEDSVVEPQGIAGSIGAGQT
                     ENLVAGTDYILDGPLIVEDGGVLNIAAGTVIKANKGFSNYILVLQGGKINVNGTAENP
                     VRMTANAATATSGYWGGLIINGKAPLAGGSTGSTEVNSAYTYGGTVAADNSGTIKYLI
                     IEYPGARSSADVEHNGLTLNGVGSGTVIENIYVLESADDAIEFFGGSVNVKNFLAVNP
                     DDDMFDFTQGYNGTLTTAYGVWAKGYTSTESDPRGIEADGNFDGNFPTQTGQSNFVVN
                     NMTIDLRLDFNTDKTTQMQDAIKIRRGAKATITNAIIKGTGSVADLVDLTDSKGAGDP
                     TSGVSLTSQLTTAPAREVSGVGVVTVGGNNTGADKAVFGWTKYAF"
     gene            complement(10389..13118)
                     /locus_tag="PLN75_03750"
     CDS             complement(10389..13118)
                     /locus_tag="PLN75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03265.1"
                     /translation="MKRKVLFRIKIVKENIFIFLLFSLASSLAAQTGSITGKVIDSKN
                     SEPLIGAAVTINGTTTGSATDFDGNYTISNLKPGTYTLTVTYISYKTLTKENTVVEAE
                     KTTLQDIALESADISLREVQVVARANRESENILLMEQKNALLATQAVGAREMSRKGIS
                     DAESAVANVSGISKQEGVKNVFVRGLGDRYNVTNLNGFPIPSEDPEYKNIALEFFGTN
                     VIQSIGVNKVFNSSLAGDVGGAVIDISSKELIGNRALGFEVSAGANPDAFQTDYLVAD
                     GANYFGFADRTRPSDGSFGFKNSLDPQKSMPLNHGFGVSGGKSFKIGENANPLSFFVV
                     ASNSKSYSLTDETVRNSNTAGTIYQDQKGRKFSNNASQLALTNVLYKFTDHQLSYNFM
                     MVHATNQYVGEYSGLNGERYQDSEDYMGFLRRQQTNDNLLITNQLITDWKLNEKMRLK
                     AGLSHNNVKGAEPDRRENYLSKDVDGSYFLTGSNRQKRFFSLLKENDFNAKINLTYAI
                     GDKFGKGNSNFQAGYSGRLVFDNFEAVEYNFTAVSGTYQIQNLSLDNIYNQSNYDAGK
                     FMMTEGDMNQYKVYKFIHAPYAEVNYQLTDKLTANAGVKADKVDMTVNHHVQHVAPGK
                     ESINKLYFLPALNLKYDANNRNSLRLGLSKTYTLPQSKEISPYQYVNISFSSQGNARL
                     KPSDNYNADLKWDFYITPSELISITGFYKHIVNPIARVDIGNSAGLLTYDNISKFATA
                     AGVELEIRKNLFSLNGNSSDKTNRLSVGLNASYIYTDLAVNYTDFNGQLRSRHTKLEG
                     AAPVIVNFDISHNYSIHKKSFVNSLVFNYFSNRIHTIGALGFKDIIEEGVPALAFVSS
                     SKINKNLTLKLKANNILNPAYRLSRENSDGTENVILNEFTKGMDLNIGITYEL"
     gene            13397..14308
                     /locus_tag="PLN75_03755"
     CDS             13397..14308
                     /locus_tag="PLN75_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRG03266.1"
                     /translation="MSSQNASVKSILFALLANFGIAVTKTVAAIMTGSGSMLAEAIHS
                     YADCANQGLLFLGLKQSKKLAEEEHPLGHGKEIYFWSFIVALMLFSMGGLFSIYEGTH
                     KLSVHEGLNRPEIAIIVLSISIILEASSLYGCITQIKKVKRKGVSLWQWTKNTRQSEL
                     VVVLGEDIAALLGLSLALIAIVLTVITGNPVFDALGSISIGVLLLIVSIFLGIKVKGL
                     LVGQSADKETREEIKALLEARPEIDEVLNAITLQLGEYIMVSVKAKMANVETDEQLIL
                     NINACEKALKTSNPAIRWVFFEPDSEE"
BASE COUNT         4228 a         3142 c         3133 g         4139 t
ORIGIN      
        1 gtataaattc gtgtaattcg tactttatga aaatattcac cacatctcaa atccggcagc
       61 tcgatgaata caccattgag catgaaccaa ttgcatccat cggattgatg gaacgcgccg
      121 ccgacaggct gcttcagcag ttcaaaaaag actggagcat gaaccgcgac gtactgatac
      181 tggcaggtcc cggcaacaac ggcggagacg gactggctct ggggcgcatg ttgcttcaga
      241 ttggttacga agttcaggta atactgatcc acggaggaaa aatttcaccc gattgcaaac
      301 aaaacaaaga ccgtttagag caatatttcc ccgatttttt caccgaacaa attaatattt
      361 tttctccacc cgaaataaca aaaaaaacca taattgtaga tgcacttttc ggttcggggc
      421 tttcaaaacc gctggaaaac acgtatgcag cagtcgttga ctggataaac cgaagcggaa
      481 atactgtggt ggccattgat attccatccg gattgtgcgg cgagcaatgt gctgctgatg
      541 ccgatccggt agtaaaagca aacctgactt atacgttgca gtttccgaaa acagcattct
      601 tttttgcgga aaacgagaag tacaccggca agtggaaagt aattgatatt cagctacatc
      661 ccggagctgt tgccaaaacc gaaacaaata tatacctcac cgaaaaggcc gatatccgga
      721 caatgctaaa acccagggaa cgattctcac acaaaggaac attcggacac acgcttatct
      781 gggcaggcaa aaaaggcatg gcaggagcag tagtgctgtc ggctaaagca gctttacgaa
      841 gcggagcagg tttggtgagt gtgcacagcg tggaagataa ccgggttatt ctgcaatcca
      901 cagtacctga agctattttt gtgagtgaat taccggattt aaccaattac aactcgatag
      961 ccataggccc cggacttggc accgattttc aaacgacaga aatgcttttt gaaatgctaa
     1021 aaacaatgta taaaccctgt gtgctcgatg ctgatgccct gaacatcatt tcgcggcata
     1081 agaatttcct tgagtttgta cccgaaaaca gcattctcac tccccacccg aaggaatttg
     1141 atagactgtt cggtgcctcg actcactcgc aggaaagaat ggaaaaagcg attacagctg
     1201 cccgggagca tgggctggtg atagtgctga aaggcgccaa cacatttatt tccacacctg
     1261 ccggaaagct atacataaat tttaccggaa atcccggaat ggccgtgggc ggaatgggcg
     1321 atgcactcac cggaattatt gcagggatgc tggctcaggg ttattcaccc gtagaaagct
     1381 caatttcagg tgtttacctg catggtttgg ctggtgacct ggcactcagt tcacaatcgg
     1441 aagaaagcct gctgccttcg gatttgattc aaaacctggg gaatgccttt actacaatac
     1501 ggaaatcgtg agatttatga acacaacata ctacttaata tactgatttt tagtacttta
     1561 tatttattta tcggatcatt caacgacata caggtacaaa ttctgaaatt ttagccctgc
     1621 aaacgaataa aacagttgaa aaaatcttac atttgtggtg agcaagtatt tacatccatt
     1681 ttttaaatcg aaaatatctg ttttgagtaa aaaaaagttc tgacaaatga aacggttact
     1741 attttttatc gtaatttttc tgacgatatt acccgtcaga tcacaggaaa tcacgctggc
     1801 agcatgtcag gaacaggcgc gagcaaattt tccgcttacc cggcagcagg gactcattga
     1861 tcaggtgaca aaaaacacca ttgaggcatt gatggcacaa tattttccgc aattccggct
     1921 cagcggacag gcgtcctatc aatctgatgt aacaaaaatt gacctgaata tgccggacgg
     1981 ttttccgaaa attgacatcc ccacacccga aaaagatcag tataaactgt acctggacgt
     2041 atcgcaaact atattcggag ggggatcact cggcgtacag cagcaattta ccaaagccga
     2101 agcggaagtg aacaaacagc gcatcgaagt ggaactttac aaacttcggg ataaaattaa
     2161 tcaggtatat ttcggtattc tgcttgccga tgagcaacag aaacaaaccg atatcctgaa
     2221 aaatgacctg aatacagcgc ttgaaaaagt gaaagtgatg gtaaaaaacg gagtcagcct
     2281 ggagaccaac cagtttaatc tggaagccga attgattacg attaatcaaa atatcaaaaa
     2341 catcgaacta ggacgctcag ccttgctgga aattctggga aaattgattg gaaaggactt
     2401 agacaaagaa actcaactga caaaaccagg caccatccga ctgaaatctg attttgtccg
     2461 tcctgaactt acactcatag acttacaaaa aaacacgctg agccttcaaa atcttcagct
     2521 tgctgctcaa aatgtgccgg aaatatcact tttcctgcaa ggcggatggg gaaaacctgc
     2581 cctgaacctt ttcagtacta aatttgagcc ctactatctc ggaggtgtga ggttacaatg
     2641 gaacatctca aggctttata ccatcctgcg cgaacgacga attattgaaa cccgcctgaa
     2701 aatgtatgat gttgagaaag atatgtttac aatgaacaac gatatcgaac aaacacagca
     2761 gctcagcaaa atcaatcaac tgaacctttc actcaaatcg gatgatgaac tgattgcatt
     2821 acgaagcaaa atcaagcaaa catcgcaggt acagctcgaa aacggtgtga ttacaagtgc
     2881 cgattttatc cgcgatgcca atgccgaaaa tcaggcccga cagaaaaaag cactccacga
     2941 aatgcaactg atactcgaac agtatcacct cttgtggatg aaagccacac cctgaacgct
     3001 caccgaaaga ggaaaaaacc gttcagcaat ccatttgctg aattttaaga aattattgaa
     3061 agataatcaa attattacgt aaacaattca aacactatat ttcaaatgaa ttcaatccaa
     3121 tccgttagca ttcaccgaaa tcttccttcc ggaggttgga tagcaggtgt attattcata
     3181 gtcttattgt taacgtcgtg taaatcaacc actaacgaag cctttgcatc cggatacttt
     3241 gaagcagtgg agaccatcgt ttcagcccgt ggaaacggaa cgatacaata cctgaacgtg
     3301 gaagaaggca tggatctgca ggcagacacc aaagtaggac agattgacag cacacaaata
     3361 tatctgacta tcagacaaat acaagcccag aaaggagcag ttctgagccg gaaacccgac
     3421 atcagctcgc aaactgctat tctgaaagag caactcgcgc acgcaaaccg cgaactgata
     3481 cgtgtggaaa acctgaacgc aggcggtgca gccacccaaa agcaactgga cgacgcccgt
     3541 tcggcagtgg cagtgctcaa ccgtcaactc agcgcacagc aaaccacact cgacaaaact
     3601 tccgcaagcg taggcaaaga aaccaatccg ctcgaagtgc agatttcgct gctgcaggat
     3661 caactcgaaa aatgccgcat tatcaatccg gtgacaggca ccgtactggt gaaatacgcc
     3721 gaacccaccg aaatgactgc catcggaaga ccgctgtaca aaatagccga tttatcgacg
     3781 ctccagttga gagcatacat taccggcaaa caattaggaa gcctcaaact gggacaaaaa
     3841 gtgaaggtga caaccgacct gggcaaagaa aaaccggctg aatacgaagg aacggtaaac
     3901 tggattagca acaaggcgga atttacaccg aaaacaattc aaacccacga agaacgctcc
     3961 aacgaagttt ttgccatcaa agtagcggta aaaaacgatg gatttctgaa aataggaatg
     4021 tacggagaaa ttgccccacc aaacctcccc aaaggggagg ctattaaaga gtaaatgtga
     4081 actctatttt ctcgttttat tgttaaattg gaggtcaaac attttcgtct gacaattaga
     4141 aaacataaat gccacttttt caaacaaata cccaatcgca cgaaccagca tcccctcttc
     4201 ggggggtggg gcggctttac atctccgtat caaacctcag caagacatac gaaaacggta
     4261 aagtgcgcgc cctggatgac gtgagtttta cggtggatga gggcgagatt tttggtttga
     4321 ttggacccga cggcgccggt aaaacttcca tattcaggat actgaccacg ctgcttatcc
     4381 ccgatagcgg aaaagcaagc gtattgggtt acgatgtagt gaaagatttc cgtaaaatca
     4441 gaaatgaaat tggctatatg cccggcaggt tttcacttta tccggattta agcgtggcag
     4501 aaaatctgaa tttttacgcc aggctgtttg gtacgaccat tgatgagaat tatcacctca
     4561 tccgcgatat ttatgttcag attgaaccat ttaaaaagcg aaaagcagga caactttcgg
     4621 gcggaatgaa gcagaaactt gccctttgtt gcgcattaat tcacaaacca aaagtgcttt
     4681 ttctcgatga gccgacaaca ggaattgatc ccgtttcgcg caaggaattg tgggaaatgc
     4741 tccggaaact gaaagacgag gggatctgca tttttgtttc gactccttac atggacgaag
     4801 ccgtaagatg cgaccgcata gctctgatac agcagggtgt aattttgaaa actgaaactc
     4861 ccgaagcgat tgtgaaacaa tacgacaaac cgatttttgc agtcggtgct gataacacct
     4921 atcaattgct taaagtgctc gaacagtatc cgaatcgcct caacagctat gcctttggcg
     4981 aatttgctca tctggtaaca aatggcaacg aagatgaact gagcgatttt ctggaaaaaa
     5041 aagggctgaa agacatcaca ataaaccgga taaaagcttc aattgaggac tgttttatcg
     5101 atttaagcat caggaatttt tctacataat acggcgcata aaaacagtaa aaaagctaag
     5161 aataattcgt ttattttcag taaaaacaga caggaaaaca gggttttcag gacacaaaac
     5221 taaccgattt ttttctaaaa aaacaagcat ttatgtattc cattacagct gaaaaactca
     5281 ccaaaaagtt cggaaatttt accgccgtcg atgctatcac gttcgaagta aaagaaggtg
     5341 aaattttcgg ttttctgggt gccaacggag ccggaaaaac cactgccatg cggatacttt
     5401 gcggcctgtc gtatcctact tccggaaaag cttcggtagc cggatttgac gtttacaaac
     5461 aacaggaaga tataaagaaa aacattggat acatgagcca gaaattttcg ttgtacgaaa
     5521 atctgacggt gagcgagaat atagaatttt acggaggcgt ttacggattg agaatgaggg
     5581 agataaggcc gaaaacgaaa aacatattgc aaaaactggg actggacagc gaagcgaata
     5641 agctggtgag ttcgctccca ctcggctgga agcaaaaact ggctttttcc acggccattt
     5701 ttcatgaacc ccggattgta tttctggacg aaccgaccag cggcgtagat ccggtaaccc
     5761 gccggcagtt ttgggaaatg atctatgaag ccgcatcacg gggcatcacg gtttttgtca
     5821 caacgcacta catggacgaa gccgagtatt gcaaccgcat ttcgctgatg gtggatggcc
     5881 ggattgatgc actggatgca ccaaaaaact tgaaagccga acacaacgcc caatcaatgg
     5941 acgaagtgtt ttatgcgctg gcgaggaaag cgaagaggag tgattagccc cctaaccccc
     6001 tgaaggggga atttgtcgca ttttgaatga gttttgataa ttcaatgaat ttgaacgtat
     6061 tttttgaatg attaataaga gaaaatataa gcaatcccac tcctcaactc ccccttcagg
     6121 gggctggggg gcaatcctta tggcatttct ccacaaggag ttcctgcatg tctttcggga
     6181 taagcggacc ttgctggtga tgttcgggct gcccattgcg cagatattga tatttggttt
     6241 tgcgcttacc aacgaggtaa aaaatgcccg gttgctgata gtaagcccgg tgcaagacac
     6301 agaaagtaag aaactgattg aaaaaattga tgccagttcg tatttcacag tcacccacat
     6361 tgttcctacg cggcaaaatc tcgaacatta tttcagaaac aacgaagccc agtgtgcgct
     6421 cattcttccc gacgattttt cggcatcggg cgtttcacct tcgcaggttc aaattgtagc
     6481 tgatgccacc gatcctaatt ttgccaaaac ggtggttttt tacctttcgg ccatcctggg
     6541 tgaatttcag caacagtcgc agcccgctca aagcattccg atggcaataa aaacagaagt
     6601 ccggatgctc tacaatccat ctctgatggg tgccatgaat ttcgttccgg gagttatagc
     6661 gctgataatg atgatagtat gcactacgct cacgtccata tccattgttc gcgaaaaaga
     6721 aacgggaacg atggaaaccc tgctcgtatc gcctctgaaa ccatttctgc tcatcatttc
     6781 caaagctata ccctacacgg tgttgtcgct ggtcaatctt tcgttcattt tgctgttgag
     6841 caatctggtg ctcgatgttc ccattcgcgg cagtattctt ttactgttgt ttgaatgctt
     6901 gttgtatatt attgcctgcc ttgcgctggg tctgatgata tccaacattg ccagttcgca
     6961 ggcagtagcc atgatgattt cgatggccgg tatactgctg cccaccttgc tactcaccgg
     7021 atttttgttt ccacttgaga atatgccgtg gatatttcag tggctctcca acgttgtgcc
     7081 ggctcgctgg tttttcatta ttgtaaaatc catcatgctg aaaggagttg gtttggaaga
     7141 tgtatggctg gaaacaaccg tactgatagg tatgaccgtt tttctgctgg ctgtaagtct
     7201 gaaacgattc aaaatacggt tggaatgaga atactcctgt tcatattgcg caaggaattt
     7261 atccagattt tcagagacag gatgatgttg cccatgctgc tgctgatgcc tctcatgcag
     7321 ctgcttattc tcccttttgc tgctgatatg gatgtgcgtc atctgaaaat tatcatcgtg
     7381 gacaacgatc acagccctgt ttcaaaaagc cttatttcaa aatttacagc ctccgattta
     7441 tttgaaattt atgctatttc gggttcatac aacgaagcat tgaaactgat tgaaaccgac
     7501 aaagccgata ttattttgca aattccgcac ggatttgagc ggaatttaat ccgtgaaggg
     7561 tccgaaaaag tggctattac ggccgatgca attaacggag tgaaagcagg acttggtacc
     7621 agctatctga atgccattat acagaatttc aatcaggaca tagcgctcga gtggagcaat
     7681 gcaacaagcc tgacaccccc tctggatgtt cgctattcgt tctggtacaa tccgtatctg
     7741 aggtataaga atttcatcgt tccggccatt ctggtgctgc ttgtaacggt tatcagcggt
     7801 tttgtgtcgg cattgaacat tgtgagcgaa aaagaaaagg gaactatcga acaaatcaac
     7861 gtcactccga taaaaaaatg ggagtttatc atcggaaaag ttttgccatt ctggttaatt
     7921 ggtatttttg atttcagtct tggtctgctt attatgtatt atgtatatgg aattcagatt
     7981 gtagggagtc ttagcacgct ttatctgttt gcaatagtgt acattaccgg tattctgggc
     8041 tttggtttgc tcatttctac ggtaagccac acgcagcaac aatccatgtt tgtggctttc
     8101 tttttcatca tgatatttat actcatgagc ggcatgttta ccaatgtgga aagcatgcct
     8161 ccgtgggctc ataccatttc cagcttaatt ccggtgacgc actttatgca cgcggtgagg
     8221 gctattgtgc tgaaaggcag cgtttttctc gacttatggg tggacatggt ttacatactg
     8281 tgttttgatg tggtggcgat tgcggcagca gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnaatacttt ttctccccgg taagttctcc attttcattc cacatacgcc
     8461 acacacccac ttttttaccg tcgttgtagt tcatttcata acgtttggtt ccattttcat
     8521 cccagatacg ccatacaccg tgttttttat cgtttttata ttcagcttcg gaaatgagtt
     8581 gaccggcagc gctgtatgag cgccacaatc catgaaactg cccgtttttg tacgaacgga
     8641 tttctttggg cttgttgttt tcaaaataga caacatacgt gccttcgggt gaaccgtttt
     8701 tgatatgcat ttcaagtttt agagagccat tatcgtaata ctccctgtaa tcgccggtat
     8761 aaagtttggt ttgagttgca tcggtgaaat aaaggccttc cttttccaca atagtgctta
     8821 cagcgggtgt ctgcgctgta gccaggattg cgatgatact catcagcaac aaataaaccg
     8881 gtttttttga gaaatacatt ttgttgagcg ttagtatatt aaaagtattt tgaaacattt
     8941 ttgaaacaaa gatataacaa ttatgaaaca ttcgttcaga aaaccttatt acaggtctaa
     9001 atttaatatg tgttacaaaa tccgggtcca accatttcaa ttgtttcata aaacagctta
     9061 cctctttctg atctgatttt caatagatta atgtcattga atacccgaga gcaaaacgat
     9121 taggaccgga cacttttttc aaaaatgctt catttttcat tttctcaaaa tgcatatttt
     9181 gtccagccaa atacagcttt atcagcacct gtgttgtttc ctccaactgt tacaactcca
     9241 accccactca cctctcttgc cggagccgtg gtcaactggc ttgtcagact tactcctgaa
     9301 gttgggtcgc ctgcaccttt ggaatctgtg aggtcaacca aatcggcaac agaaccggta
     9361 cctttgataa ttgcattggt aattgtggcc tttgctccac ggcgaatttt gatggcatcc
     9421 tgcatttgtg tggttttgtc ggtattgaaa tcgagtctca gatcaatagt catgttattt
     9481 accacgaagt tagactggcc ggtttgggtc gggaagtttc cgtcgaaatt tccatcggct
     9541 tcgataccgc gcggatcgga ttctgtactg gtataacctt ttgcccatac gccgtaagcg
     9601 gttgtaagcg ttccgttata gccttgcgta aagtcgaaca tgtcatcatc cggatttacg
     9661 gccagaaagt tcttaacatt tacagagcca ccgaagaatt caatggcgtc gtcggcactt
     9721 tcgagtacat atatattctc gattacggta ccactgccta caccattcaa tgtaagtccg
     9781 ttgtgttcca catcagcact tgagcgtgca cccggatatt cgattatcag gtatttgata
     9841 gtacctgaat tatctgctgc aacggtacct ccgtaagtat aagcagagtt cacttcagta
     9901 gatccggtgc ttccgcctgc cagcggggct ttaccattga taatcagacc gccccagtaa
     9961 cctgaagttg ctgtagcagc gtttgcagtc atccgaacag gattttcagc agtgccattc
    10021 acattgattt taccgccctg aagtaccagg atgtaattgc tgaatccttt atttgctttt
    10081 attaccgttc cggctgcaat gttgagcacg cctccgtctt ccacgatgag cggaccatcc
    10141 agaatataat cagtgcctgc aaccaggttt tcggtttgac ctgctcctat cgaacctgca
    10201 ataccttgtg gttcaaccac ggaatcttcc ggtttacagg aattaaatgt tagtgacatc
    10261 atcactactg ttattgccat caggcttaaa ataaactttt tcattttttt taatttttaa
    10321 tgagtttaag aaaattatac gatttgtttt atatgtttag aggtttttga ttaaaaatca
    10381 atcattgttt atagttcgta cgtgatgcct atgttcaggt ccattccttt tgtgaattcg
    10441 ttgagaatga cattttcagt cccgtcggaa ttttcgcgtg atagcctgta tgccggattc
    10501 aggatgttgt ttgctttgag ctttagcgtc agatttttat ttattttgga tgaagacaca
    10561 aaagccagag caggaacacc ttcttcaata atgtctttga aaccaagcgc tccgatggtg
    10621 tgaatgcgat tactgaaata gttgaatacg agcgaattga caaaactctt tttgtggatt
    10681 gaatagttgt gtgaaatatc aaaattcaca attacaggcg cagcaccttc gagtttggtg
    10741 tgtcgggaac gcaactgacc attgaaatcg gtgtaattaa ctgccaaatc agtataaatg
    10801 tacgaagcat tcagtcctac tgataatctg tttgttttat cgctgctgtt tccattgagc
    10861 gaaaacagat tttttcgtat ttcaagttcc actccggctg ctgtggcaaa tttactgata
    10921 ttgtcgtagg taagcagccc ggcggagtta ccaatgtcca cacgtgctat cggattaaca
    10981 atatgcttgt agaatccggt gattgaaatc agttcggaag gggtaatgta aaagtcccat
    11041 ttcaggtcgg cattgtagtt atccgacggc ttgagtctgg cattaccctg gctggagaaa
    11101 ctgatattga catactgata gggtgaaatt tcctttgact gaggcaatgt gtatgtttta
    11161 ctaagtccca gacggagtga attccggttg tttgcgtcgt atttcaggtt tagtgcaggc
    11221 agaaaataaa gtttgttgat gctttctttc cccggagcca cgtgctgcac gtgatgattc
    11281 acagtcatat ccactttatc ggctttcaca ccggcgttgg cagtcagttt atctgtcaac
    11341 tgataattca cttctgcgta aggagcgtga ataaacttat acactttata ttggttcata
    11401 tctccctcgg tcatcataaa ttttccggca tcataatttg actgattgta gatgttatcc
    11461 agactcaaat tttgaatttg ataagttccg ctcactgccg taaagttgta ttccacagct
    11521 tcaaaattgt caaaaaccaa acgtccgctg taacctgcct gaaaatttga attacctttc
    11581 ccaaacttat ctccgattgc ataggtcaga tttatttttg cattgaagtc attttctttt
    11641 aataatgaaa aaaagcgttt ctggcggtta ctgccggtga ggaagtagga tccgtccaca
    11701 tctttcgaca gataattctc cctgcgatcg ggttcggcac cttttacatt attatgcgac
    11761 aaacccgctt tcagacgcat tttctcgttt agtttccagt cagtaatcag ctggttagta
    11821 atcagcaggt tatcatttgt ctgctgacga cgaagaaaac ccatgtaatc ttcgctgtcc
    11881 tggtagcgct cgccgttaag cccgctgtat tctcccacgt actgattggt agcgtgcacc
    11941 atcatgaaat tgtaacttaa ctgatgatct gtgaatttgt aaagtacatt ggtgagagca
    12001 agctggctgg cattgtttga aaatttcctg cctttctggt cctgataaat ggtgccggca
    12061 gtattggaat ttcgaactgt ctcgtcggtc aacgagtagc ttttactgtt cgaagccaca
    12121 acaaagaaag aaagcggatt ggcattttct ccgattttga aactttttcc tcctgaaaca
    12181 ccaaaaccgt gattcaaagg cattgatttc tgtggatcca ggctattctt aaaaccaaaa
    12241 ctgccatccg aaggacgtgt ccggtcggca aatccgaaat agttggcgcc gtcagccacc
    12301 agataatcgg tttggaaggc atccggattt gctccggcag atacttcaaa tccaagtgcc
    12361 cggttaccaa tcagttcttt cgatgaaata tcaataacag ctcctcccac atctccggcc
    12421 agcgaactgt tgaatacttt gtttaccccg atactttgaa tcacgtttgt tccaaaaaac
    12481 tcaagtgcaa tatttttata ttcgggatct tccgatggaa tcggaaatcc gttcaggttg
    12541 gttacgttgt agcggtcgcc cagtccgcga acgaacacgt tttttacccc ttcctgcttt
    12601 gaaattcctg aaacattggc cacggcgctt tcagcatcac ttatcccttt acgcgacatt
    12661 tctctggctc ccactgcctg tgtggcgagt aacgcatttt tttgttccat cagaagaatg
    12721 ttttcgcttt cgcgatttgc tctggccact acctgcactt ccctgaggct gatatcggcc
    12781 gattccagtg caatatcctg caaagtagtt ttttcggctt ctacaaccgt attttctttc
    12841 gtcagcgttt tgtatgaaat ataagtgacg gttaatgtat atgtgcccgg tttcaggttg
    12901 ctgatggtat aatttccgtc aaaatcggtt gcgcttcctg ttgttgtacc gttgatggtt
    12961 acggctgcac caataagcgg ttcgctgttt tttgagtcaa tcactttacc ggttatacta
    13021 ccggtttgtg ctgccaggct tgaagccaga gagaaaagga gaaaaataaa aatattttct
    13081 tttacaattt taattctgaa taaaactttt cttttcatta aaatatgttc ataaaatttc
    13141 ggtgcaaaat tccttgtttt atgttacagc aaggtgtaat tgatgttaca tttcctgaaa
    13201 gacatattac aataccatta tagctccagc tgctatcaac agaaaggaac tgtaagtgaa
    13261 tacagaattg agaaaacggg tacaatttgt gaattactgt tgtacgctta cattttccat
    13321 tgtaaaacac ttgccatgaa accgtttgtg tctgaattct tttttttact tttgcattcg
    13381 ttaatacaat actaatatgt catcacaaaa cgcttctgtc aaatccattt tattcgcgct
    13441 gctggccaat tttggaatag ccgttaccaa aactgtggct gcaattatga ccggctcggg
    13501 ctccatgctg gccgaagcca tccactccta cgccgactgt gccaaccagg gtttgttgtt
    13561 tctcggactg aaacaatcca aaaagctggc agaagaagag catccgctgg gtcatggcaa
    13621 agaaatctac ttctggtcgt tcattgtggc gctgatgctt ttcagcatgg gtggtttgtt
    13681 ttccatttat gaaggaacgc acaaactgag cgtacatgaa ggactcaatc gccccgaaat
    13741 tgcaattatt gtattgtcta tcagcattat tttggaagcg tcctcgctct acggatgcat
    13801 tactcaaatc aaaaaagtga aacggaaagg tgtttcgctc tggcaatgga caaaaaacac
    13861 acgtcaaagc gaattagtgg ttgttttagg tgaagatatt gctgctttgt tgggtttgag
    13921 tttggcttta attgccatcg tattgacagt tattaccgga aatcccgtat ttgatgccct
    13981 tgggagcata agcattggtg ttttattgtt gattgtttcc atctttttgg gaatcaaagt
    14041 aaaaggattg ctggtaggtc aaagcgccga caaagaaacc cgggaagaaa taaaagccct
    14101 gctggaagca agaccggaaa tagatgaggt gttgaatgct atcacactgc aattgggtga
    14161 atacatcatg gtttctgtta aagcaaaaat ggccaacgtg gaaaccgacg aacagctgat
    14221 tttgaatatc aacgcttgcg aaaaagcact aaaaacgtcg aatccggcaa tccgatgggt
    14281 gttttttgag cctgattcgg aagagtagtt cgtaatgttc taatgtattt gacaaaatta
    14341 tatcaccgtt tttacacatt tcctttacac atttgaaata ttccgatgat aaatttgcag
    14401 agaataaaac tcacgagaat tctctcataa tttttcattt gagaaacaac gactttgcga
    14461 aaagccgttg ttttttctgt ataacaccct taattggaac agaaatttga agttttttga
    14521 actcctgctt cattccggta aaaaatatac taaataaacg aatcagaatt cgatattaat
    14581 aaaaaatgtg tatttttgta aaaaaccaaa aaagcagtca tcgtatgaaa cagccattaa
    14641 acacaaattt tcacccatgg gaatgaagat agatggcgtg ttccattcgt ccttaattta
    14701 aaaaaataaa aaatcttcgt atgaaagcaa tgatttttgc cg
//
LOCUS       DAORKP010000041        14567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_334_length_14567_cov_10.038313, whole genome shotgun sequence.
ACCESSION   DAORKP010000041 DAORKP010000000
VERSION     DAORKP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_14567_cov_10.038313"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..734
                     /locus_tag="PLN75_03760"
     CDS             132..734
                     /locus_tag="PLN75_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HRG03267.1"
                     /translation="MKSSLIILSFFALGLLLGVFRMLPQQWVDSSITFYVLCLLMFSV
                     GLSLGHDKDSVRKFRSLSPRLLLLPAMTVLGTFLGVTAVSVLIKGRTFSELLAVSSGF
                     GYYSLSSVLITQYKGVELGAIALLANIIREVITLLFAPWMVRFFGPLAPVSAGGATTM
                     DTTFPVIIRNSGKEFSILSIYHGFITDLSVPFLVTFFCSL"
     gene            complement(908..1594)
                     /locus_tag="PLN75_03765"
     CDS             complement(908..1594)
                     /locus_tag="PLN75_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRG03268.1"
                     /translation="MKNRFIFTTFILLFTATAFGQVGLRGGINTASEIHSFSQNDISA
                     AFQGENLTGYQVGLVYQFNPKRSGLGFEIGALLSQKGSVFRMDSASIVQSLIKGYHEI
                     NYVEVPINLRLRLSFGGIIGVFGSAGVYGAYALRGKTVFESDIATLIQEESFDSFMNR
                     IDYGYSYGGGVELLKKIQLALQWSQGLQKRDANKNILDKITTESGGLAPNLTAKSSSK
                     VFSVSLTYLF"
     gene            complement(1632..3431)
                     /gene="typA"
                     /locus_tag="PLN75_03770"
     CDS             complement(1632..3431)
                     /gene="typA"
                     /locus_tag="PLN75_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRG03269.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKLLLAGHLFRDNENAGELIMDN
                     NDLERERGITILAKNVSINYQGHKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALSIGLKPIVVVNKVDKPNCRPDEVNEMVFDLMYNLNATEEQLDFK
                     TVYGSAKQNWMSDDWKNPTTDITPLLEAIIKYIPAPTQLQGTPQMLITSLDFSPYVGR
                     IAVGRIHRGILKEGMDVSLCKRDGSIVKSRIKELDTFTGLGRTKTDVVESGDICALVG
                     IDGFEIGDTIADLQNPEPLAPIAIDEPTMSMVFTINDSPFFGKEGKFVTSRHINDRLI
                     RELDKNLALKVERNPNSDVWNVFGRGVLHLSVLIETMRREGYEMQVGQPQVIFKEIKG
                     VRCEPVEQLTINLPEENSGRIIEMVAVRKGEMISMEAVGDRIQLEFHIPSRGIIGLRN
                     SVLTATAGEAIMSHRFVEYQPYKGEIERRTNGSIIAMESGTAFAYAIDKLQDRGKFFI
                     HPQDPVYTGQVIGENAKEGDIVVNVCKSKKLTNMRASGSDDKVRIVPPIIFSLEEALE
                     YIKEDEYVEVTPTKIRLRKILLDENDRKRLSKK"
     gene            complement(3648..3720)
                     /locus_tag="PLN75_03775"
     tRNA            complement(3648..3720)
                     /locus_tag="PLN75_03775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3685..3687),aa:Phe,seq:gaa)
     gene            3928..5415
                     /locus_tag="PLN75_03780"
     CDS             3928..5415
                     /locus_tag="PLN75_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRG03270.1"
                     /translation="MISIELFLLLISVLLFVSLLVGKMGSRFGVPTLLLFLLIGMLSG
                     SDGLGIEFNSPKIAQAIGVVALNIILFSGGMDTRFTEVKPIAAQGMILATIGVLFTAL
                     LTGIFIFWLTNNFFNAVTFTLLESLLLASVMSSTDSASVFSILRSKNLSLKENLRPLL
                     EFESGSNDPMAFMLTIVLIQLIQSPVIDAWAALWMFFKQLLLGGLAGYFLGRLSVRII
                     NKINLDNDALYSVLLMTLMFFLFGFTTFISGNGYLAVYVGGLFIGNSRFVHKRSSLKF
                     FDGMTWLVQIIMFLTLGLLINPSELLPIAGIGIIVGLFMIFFSRPVSVHLSLLPFRNL
                     SHKARHYVSWVGLRGAVPIIFATYPWIENLPHAKDIFNIVFFITILSLVIQGTTVGSM
                     AKWLGLSQDVLRKRKLKNFDVEISEDIKSYMSEISIKKEFLANGSRLMDLNVPEKTLI
                     AMVKRDDQYFIPRGNTELMVGDRILVITDNEKALEQTYKSMKGEM"
     gene            5634..9413
                     /locus_tag="PLN75_03785"
     CDS             5634..9413
                     /locus_tag="PLN75_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRG03271.1"
                     /translation="MQSGDINAQSPSVKPDASIRIEQVRKQNAQTAEIFFTNGQKMIL
                     DFYGENIFRMYQNNNLNVLADPQADPPARILVENSRKSIGKLEITTDNASVSILTNRI
                     KLVFDKETTCFTISDLLTGKTVVQSTSPVSFEKNEVKLTLKEHPDEYFFGGGVQNGRF
                     SHKGKTIAIENQNSWTDGGVASPTPFYWSTNGYGMMWYTFRPGVYDFGRKEKNTVQLT
                     HKTDYLDVFFMVNKETTGLLNDFYQLTGNPVLIPKFGFYEGHLNAYNRDYWKEDAGGI
                     LFEDGKRYRESQKDNGGVKESLNGELNNYQFSARAVIDRYKQHDMPLGWILPNDGYGA
                     GYGQTETLEGNIVNLKNFGDYARKNGVEIGLWTQSDLHPKEGVEPLLQRDIVNEVGVA
                     GVKVLKTDVAWVGAGYSFGLNGVQDVASIMPKYGNFARPFIISLDGWAGTQHYATIWS
                     GDQTGGVWEYIRFHIPTYIGSGLSGQPNICSDMDGIFGGKNPVVNIRDYQWKTFTPMM
                     LNMDGWGSNEKYPHALGEPAASLNRNYLKLKSELLPYAYSVSREAIDGLPVIRAMFLE
                     EQNLFTLGINTQYQFMFGPNFLVAPIYQNTRADEQGNDLRNNIYLPEGEWIDYFSGQK
                     YEGGRIINNFESPIWKLPVFVRNGAIIPMTNPNNNPTEIDKSHRIYEIYPHGNTSFTE
                     YDDDGETTAYLSGKFVETQINSSVSKNQATITIQPLKGDFKGFVKEKTTEMKVNVTAK
                     PTGIAAKVGNRKVRLKNARTMQEYNSATNVYFYDAAPDFNRFATKDSEFARVKIIRNP
                     QLLVKIGKTDVSVNDVKLIIKGFEFEPTNPLLQHAGKLTPPQNIAVSDSNATAYTLRP
                     EWSAVPGADYYELEFGGMLYSTIRENSLLFENLTPETRYAFKARAVNKSGASEWTGFE
                     ATTKSDPLQFAIRGIKAQSNVEMQGGEGLRRLFDFDEGNMMHTKYGENAVPFDLTLDL
                     NSVNQLDKLEYLPRTSGQNGMFEKGKILFSNDKQTWIEAGEFEWKRNGENKTFVFEKQ
                     PVARYIKISVEKSVGNFGSGREIYIFKVPGTESYLPGDINNDKLIDSNDLTSYLNYTG
                     LRLGDSDFEGYVSRGDVNKNNLIDAYDISNVAVRMDEAESDEDNGIVEGTLLLTADKK
                     NYREGETVEITVRGQNLKSVNALSFSLPYNNNYEFTGIRILNTGEMQNFTNDRLHSDG
                     EKVLYPTFINVGNQKTLEGNADLFVIRLKAKRALKFELTPQKGLLVGKNLSSLAF"
     gene            complement(9568..10242)
                     /locus_tag="PLN75_03790"
     CDS             complement(9568..10242)
                     /locus_tag="PLN75_03790"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRG03272.1"
                     /translation="MKRINILLSFLLMITVFSFGQFKLPSLPYEYGALEPFIDSTTMR
                     IHHTAHHAAYVNNLNKAVEKYPQYNKLDITELLRKINTLPKDIQTAVRNNGGGHYNHT
                     FFWEIMAPAGTTTMSPALEKALVKQFGSVDKFREEFEKAATGRFGSGWVWLMQKSDGT
                     LYIDSTPNQDNMLMPFNTMKGKPILALDVWEHAYYLKYQSKRAAYVKAFWNVVNWNKV
                     SELAGL"
     gene            10446..13268
                     /locus_tag="PLN75_03795"
     CDS             10446..13268
                     /locus_tag="PLN75_03795"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRG03273.1"
                     /translation="MKKYLILSFCFAMSFALQAQHAVKSKIISKSTGEPLEFVAVRLL
                     NTDSALVAGQSTDSLGEFTLKDIKSGKYILQATSVGYIAINRNLEMRSANVVLENIQM
                     EDDSKVLKELEVAGTAVQVVTKGDTIEYNATTFKTGQNAVVEDLLKKMPGVEITSDGK
                     IMVNGQEVKKIRVDGKKFFGDDMEMSTKNIPADMVDKVQVVDQKSEMSQLTGFDDGET
                     ERIINLTIKRDRRQGVFGNVQAGAGTDINPEFRYDANAFLNIMNGDTRSSVTAGANNT
                     NTARSRRGRGGFGGGRSGITATQNLGYNINTPLSKQLIVGGDVTFNHSDNLVLSESQR
                     ENYLQDATYQNNSNRMSNRENYQGNLRLEMEWKPDTMNSFIIQPNIGFNRSFSRSTSD
                     YLYLKENDSTSWGNTKNSGDGLDRDAGITFIYNRKLAKPGRTFTTRLNTNMSASDDNG
                     MNYSRKITNDSTILIDQRSDNTSNNFNFGLRLSYVEPLGTSRKHFLETSLSFNGNIRN
                     SVRDLFDQDVNGNYIVKNNEYSNEFSNRFFRETLDLNYRYVMPAYNLTLGFSAEPSQT
                     YSTTIYGDNTGVPIKNEVVNLAPAARFQYNFGKRNFARLEYRGRTEQPSISQMQPVKN
                     NTDLMNETVGNPALNPAFNHNLRLMYSNYNAETFSSYSVGVFGNATKDNLTSNSIYDN
                     TGKRYIQTVNSKEIPYNLNAFFMYNQPFLKKFNFSNNTSFGTRQQYGYTSKNVNAADI
                     NLNNLLIGDLSSTIRYNAAENISMSFTHEIVDVALRGGLGYSYSKNNFNKKASETYDW
                     TSALNFGLRPTKTLTFTTDLNFTKQNGYADFNPSQWIWNASLDLTAFKAKGVFSLKVF
                     DILRQQQNINQNVGDNFVEFSKSNALPSYFLVSFTYKINKFKGGNSQDRENLENMNRM
                     GPGMGRGMRMGGGGPDGGAPPPPPFM"
     gene            13350..14276
                     /locus_tag="PLN75_03800"
     CDS             13350..14276
                     /locus_tag="PLN75_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRG03274.1"
                     /translation="MTKIRIAFLGLGGVGGYYGGKLAARYSGSGRVEVCFFARGEHLK
                     AIQETGIHIITDEEDFIARPDLATDNPQDAGVVDYVIITTKSYDLRSSIEFIRPCIGP
                     HTIILPLLNGGDITERIREILPENIVWSGCSYIVSRKTGPGVIRATGTFYKLVFGYDK
                     EPNEQLQNFEKLLQEARIDAKLSVNIRESIWKKFFFISVSASLTSYFDVGFNELVDTP
                     DRLNMTVGLAEEFLKVAKAEGINLGENAIEQVVNRAEVLPAGTTTSMHSDFKAGNRTE
                     VETLTGVIVQLAQKHRIYTPLYETVYNSLKNR"
     gene            complement(14355..>14567)
                     /locus_tag="PLN75_03805"
     CDS             complement(14355..>14567)
                     /locus_tag="PLN75_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRG03275.1"
                     /translation="SEFVIDFIRIMPGTPKANVKSRIILTPEHAKRLLFALQDNISKY
                     EQQHGKIKTPENNMMPPLPMGFGGEA"
BASE COUNT         4126 a         3267 c         3233 g         3941 t
ORIGIN      
        1 tatgaaacag ccattaaaca caaattttca cccatgagaa tgaagtcccg atagctatcg
       61 ggaggcgtgt tctcccgata aatcgggaca ggctatacgt ccttaattta aaaaaaataa
      121 aaaatcttcg tatgaaaagc agtctgatta tattgtcatt cttcgcgctg ggtctcttgc
      181 tgggcgtttt cagaatgctg ccccagcaat gggtggattc gagcattaca ttttatgtgc
      241 tgtgtctgct tatgttcagt gtgggtttga gcctcggtca cgacaaagat tcggttcgta
      301 aattccgctc gctctcaccc cggttgctct tgttgccggc tatgaccgta ttgggtactt
      361 ttcttggagt gacggcggta agtgtgctta ttaaagggag aacattcagc gaattgctgg
      421 cagtgagttc cggattcggg tattactccc tctcgagtgt gcttatcacg cagtacaaag
      481 gtgtagaact gggtgctatt gccctgcttg ccaacattat acgcgaggtg attacgcttc
      541 ttttcgctcc gtggatggta cggtttttcg ggccgttagc gccagtttcg gccggtggcg
      601 ccactaccat ggacactacg tttccggtca tcatcagaaa ttcgggaaaa gaattttcaa
      661 tactttctat ctatcacggt tttattaccg atttgtcagt tccttttctg gttacctttt
      721 tttgcagttt gtaattttcc ggatcagatt caatgaataa cacgatgttt ttcgtatcat
      781 ttgttgaatt ggttgttgaa aaaataaggt gaattcgata gtgtgatcaa ccataccccg
      841 acaaaaaaac tgcctgtggg aggcagaata ttgtgagaaa tctgaataac acactaagac
      901 cgtacttcta aaaaagatag gtaagtgata ccgaaaatac ttttgatgat gatttggcag
      961 tgaggtttgg ggctaatccg ccgctttctg ttgtaatttt atccagtatg tttttattag
     1021 cgtctctctt ttgcagcccc tggctccact gtaatgccag ttgaattttt ttaagcagct
     1081 ctacaccacc accgtagctg taaccgtagt caattctatt cataaaactg tcgaatgatt
     1141 cttcctgaat caacgttgca atgtcacttt caaaaacagt ttttcctctc agggcataag
     1201 ctccgtaaac gccagctgag ccgaatactc caattatccc tccgaaacta agtctcaatc
     1261 ttaagtttat gggcacttcc acgtagttta tttcgtgata tccctttatc agcgattgaa
     1321 caatactggc actgtccatt ctgaaaacac tccctttctg cgatagcagc gctcctattt
     1381 caaatccaag tccagatctt ttcggattaa actgatacac caatcctacc tgatatccgg
     1441 tcagattttc gccctgaaat gctgccgaaa tgtcgttttg actgaatgaa tggatttcac
     1501 tggccgtatt gatgcctccc cgaagcccga cctgcccgaa agcagtggct gtaaacagga
     1561 gaataaaagt tgtgaaaata aacctgtttt tcatgtttga gacgtgtttt tttttcgatg
     1621 aaaatctcca gttatttttt tgataaccgt ttccggtcgt tttcgtcaag tagtattttt
     1681 cgaagcctga ttttagtggg agttacctct acatattcat cttccttgat gtattcaagg
     1741 gcttcttcca ggctgaaaat aatgggtgga acgattctta ccttatcgtc cgaacccgaa
     1801 gcgcgcatgt tggtcagctt tttcgatttg catacattca ccacgatatc gccttctttg
     1861 gcattctctc caatcacctg gccggtgtat accgggtcct gtggatgaat gaaaaacttg
     1921 ccgcggtcct gtagtttatc tatggcgtag gcaaatgctg taccactttc catggcaatg
     1981 atggagccgt ttgttcgccg ttctatttct cccttgtaag gctggtattc cacgaatcgg
     2041 tgcgacatga tggcttcccc ggcagttgct gtcaacacgc tgttgcgcag cccgataatg
     2101 cctcgtgaag gtatgtgaaa ttcaagctga atacggtcgc caactgcttc catgcttatc
     2161 atttcgccct tgcgtaccgc taccatctcg ataattcgcc ccgaattttc ttcgggtaaa
     2221 ttgatggtaa gctgctctac aggttcgcag cgtactcctt ttatctcttt gaaaataacc
     2281 tgcggctgac ccacctgcat ttcatagccc tcacgtcgca tggtctcaat aagtaccgac
     2341 aggtgcagca ctccgcggcc aaacacgttc caaacatccg aatttggatt tctttctact
     2401 ttcagtgcca ggttcttatc cagttcgcgg atgagccggt cgttgatgtg gcgcgaggtg
     2461 acgaattttc cttctttgcc gaagaacggc gagtcgttga tggtgaaaac catgctcatc
     2521 gtaggttcgt caatggcaat gggggccagc ggttcggggt tttgcaggtc ggctatggta
     2581 tcgccgattt cgaaaccgtc aattcctacc agcgcacata tatcgcccga ttccactacg
     2641 tcggtttttg tccggcctaa acccgtaaag gtgtctaatt ccttaatcct tgattttacg
     2701 atagaaccgt cacgtttaca aaggcttaca tccattcctt ctttcagaat gccccggtga
     2761 attctcccca cggcaattct ccctacgtag ggcgagaagt ccagcgaagt gatgagcatt
     2821 tgcggagttc cctgaagttg agtgggagcg ggtatgtatt tgatgattgc ttccagtagg
     2881 ggagtgatgt cggtggtcgg atttttccag tcgtccgaca tccagttttg cttcgccgag
     2941 ccgtacactg ttttgaagtc gagctgttct tcggtggcgt tcaggttgta catcaggtca
     3001 aaaaccattt cgttcacttc gtcggggcgg cagttcggtt tgtccacctt gttgaccacc
     3061 actatcggtt tcaggcctat ggaaagtgct ttttgaagta caaagcgtgt ttgaggcatc
     3121 ggtccttcga aagcatccac cagcagcagc acgccgtctg ccatgttgag cacccgttcc
     3181 acttcgccgc caaagtcggc atggccggga gtgtcgatga tatttatttt atgtccctga
     3241 tagttgattg atacattctt tgccaatata gttataccgc gctcacgttc caggtcgttg
     3301 ttgtccatga taagttcgcc ggcattctcg ttatcccgaa acaaatgtcc ggccagcagc
     3361 agtttatcaa ctaatgtggt cttaccgtgg tcaacgtgag cgataatagc tatattcctt
     3421 attttttgca tacagtaata tttggttttg cgggtgcaaa gatagtgaaa aacagtggaa
     3481 atttgtgtta atggatattc ctaatttgaa caggcagcaa agaatgtgtt tggaatctat
     3541 tgttttataa aaaaaaagta agttaaacat tgaaaatttt gtggtgcaaa ttcataaaac
     3601 caaaaaacgc aactgattga ttatcagctg cgttttcgtt ttgtaagtgg taccaccagg
     3661 aatcgaaccg gggacacaag gattttcagt cctttgctct accaactgag ctatggtacc
     3721 tctttttgat tttgagagtg caaatgtacg gcaacttttt caaagaaaaa aatatttgac
     3781 caaaaaataa tcgggtttat tatatttttt gaaaaaaaca tgtcagcaat ttattcttaa
     3841 aaaaccaaat aattcaaaat gaatagtttt acaaaacggg gaaaaaagtt aactttgcag
     3901 aagattttat attcttaaaa ttttacaatg attagtatcg aactgttttt gttgttgatt
     3961 tctgtcctgc tttttgtcag tttgctggtt ggaaaaatgg gtagtcggtt tggtgtacct
     4021 accttattac ttttcctgct gataggaatg ctgtcgggta gcgatggact tgggattgaa
     4081 tttaattctc cgaaaatagc tcaggccatt ggagtggtag ccttgaatat tatacttttt
     4141 tcgggtggta tggatacgcg gttcactgaa gtgaaaccca ttgcagctca gggaatgatt
     4201 ctggcaacca ttggagtgtt gtttacagct ttgttgacag ggatttttat tttctggctc
     4261 accaataatt tcttcaatgc ggttacattt acgctccttg aatcattgct tttggcaagt
     4321 gtaatgtcat ctaccgactc ggcttcggtt ttttctatcc tgcgttcgaa aaatctttca
     4381 ttaaaagaaa acttgcgtcc gttgcttgaa tttgaaagcg gaagcaacga tccgatggct
     4441 ttcatgctca ccattgtgct cattcaactg attcaatcgc ccgtaatcga tgcgtgggct
     4501 gctctctgga tgttttttaa gcagctgctg cttggtggtc tcgcaggtta ttttctgggt
     4561 agattgtcag tacggattat taacaaaatc aacctggaca acgacgcact ctattcggta
     4621 ctgcttatga cactgatgtt tttcctcttc ggatttacca cttttatcag tggaaacggt
     4681 tatctggcag tatatgttgg ggggttattt ataggaaaca gccgttttgt acataagcgc
     4741 agttcgctga agttttttga tggaatgacc tggctcgtac aaataatcat gtttctcacg
     4801 ctcggtttgc tcatcaatcc gtccgaatta cttcctattg ccggaattgg tataatcgtc
     4861 ggtttgttca tgattttctt ttcacgaccc gtttcggttc atctcagttt attgccgttt
     4921 cgtaatctgt cgcacaaagc caggcattat gtttcgtggg taggactccg gggtgctgtt
     4981 cccattattt ttgccactta tccgtggata gagaatttgc cccacgccaa agacattttt
     5041 aatatcgtgt tttttatcac cattctttcg ctggttattc agggcaccac agtaggctcc
     5101 atggccaaat ggctgggtct ttcgcaggat gttctccgca aacggaaact taagaatttt
     5161 gatgtggaaa tttcggaaga tatcaaatct tacatgagcg aaatttccat aaaaaaagag
     5221 tttctggcca acggctcgcg gctgatggat ttgaatgtgc ccgagaaaac cctgattgcg
     5281 atggtaaaac gtgatgatca gtatttcatt ccccgcggaa ataccgaatt gatggtaggc
     5341 gacaggatat tggttattac agacaacgaa aaagcactgg aacaaaccta taaatctatg
     5401 aaaggtgaga tgtgattttc gccttttatt attttaaaat ttcttttgaa taaactcttt
     5461 cttttttttt tgcatccgct tcttaaggag aagaggcagg agataaagta gtacacaaag
     5521 aaaaagaaca ggttttcaat tttttatgac cttatatact ataaaaaaaa taaatcacaa
     5581 tgaagaaaat taaaacattc attcctttaa ttgttttgtc attgttcctg agcatgcaat
     5641 ccggggatat taacgcacaa agtccttccg tcaagccgga tgcctccata cggattgagc
     5701 aggtccggaa acaaaacgcc caaacagcag agattttttt tacgaacggg caaaagatga
     5761 ttctcgactt ctacggagaa aatatctttc ggatgtatca gaataacaac ctgaatgtgc
     5821 ttgccgaccc tcaggctgat ccgcctgccc gaatcctggt ggaaaattcc cgtaaatcaa
     5881 ttggtaaact ggaaattaca accgataatg cttccgtttc aatcctgact aaccggatta
     5941 aactcgtttt tgataaggaa accacctgtt tcacaatcag cgatttactc accggtaaaa
     6001 ccgtcgtgca atccaccagt cctgtttcat tcgaaaaaaa cgaagttaaa ctcacactga
     6061 aagaacatcc ggatgaatac tttttcggag gtggagtgca aaacggacgt ttttcgcaca
     6121 agggaaaaac cattgccatc gaaaatcaga acagctggac cgacggcgga gtggcttcgc
     6181 cgacaccctt ttactggtca acaaatggct atggaatgat gtggtacacc ttcagaccgg
     6241 gtgtatacga ctttggaagg aaagagaaaa atacagttca gctgactcac aagaccgact
     6301 acctcgatgt tttcttcatg gtcaacaaag aaacaaccgg cttactcaat gatttttacc
     6361 aactcacggg caatccggtc ttaataccga aattcggctt ctacgagggg catctgaatg
     6421 cctacaaccg cgattactgg aaagaagatg cgggaggaat cctttttgag gacggcaagc
     6481 gctacagaga aagtcagaaa gacaacggcg gagtgaaaga aagtctgaac ggtgaactga
     6541 acaactacca attctcggca cgcgcggtaa tcgaccgtta caaacaacac gatatgccgc
     6601 tgggctggat tttgcccaac gacggatatg gtgcgggtta cggacaaacc gaaacgttgg
     6661 aaggaaacat cgtgaatctg aaaaattttg gcgattatgc ccgaaaaaac ggagtggaaa
     6721 taggtctgtg gacacagtcg gatcttcacc cgaaagaagg agtggaaccg ctcttgcaac
     6781 gcgatatagt caatgaagtg ggcgtggcgg gagtaaaagt gctcaaaaca gatgttgcct
     6841 gggttggagc aggatattcc ttcggattaa acggagtgca ggacgtggcg tccattatgc
     6901 caaaatacgg taactttgca cgtccgttca tcatttcact cgacggatgg gccggaaccc
     6961 agcattacgc aaccatctgg tcgggcgacc agaccggcgg tgtgtgggaa tacatccgct
     7021 ttcacattcc cacgtatatc ggttcaggat tatccggaca gcccaacatc tgttcggaca
     7081 tggatggcat tttcggagga aaaaatccgg ttgtcaacat ccgcgattat cagtggaaaa
     7141 ctttcacacc catgatgctg aatatggacg gttggggaag caacgaaaaa tacccgcacg
     7201 cactcggcga accggctgca tctctcaacc ggaattacct gaaactgaaa tcggaacttt
     7261 tgccctacgc atacagtgtc tcacgcgaag ccatcgacgg gcttccggtc attcgtgcca
     7321 tgtttctcga agagcaaaat ctgtttacac tcggaataaa tacccagtac cagttcatgt
     7381 tcggacccaa ttttctggtg gcacccattt atcagaatac ccgcgccgac gagcagggaa
     7441 acgacttacg caataacatc tacctccctg aaggtgagtg gattgactat ttctcaggtc
     7501 agaaatacga aggcggacga attataaaca attttgaaag tccgatatgg aaactgcccg
     7561 tgtttgtcag aaacggagcc atcatcccga tgacaaatcc taacaacaat ccgacagaaa
     7621 ttgacaagag ccaccggatt tatgaaattt atcctcacgg caatacctca ttcacagaat
     7681 acgacgacga tggggaaact accgcttacc tgagcgggaa gtttgttgaa acacaaatca
     7741 attcatcggt ttctaaaaac caggcaacca ttaccattca gcctctaaaa ggcgatttca
     7801 aaggttttgt aaaagaaaaa acgaccgaga tgaaagtgaa tgtaaccgcc aaaccgacag
     7861 gaatagctgc aaaggtcgga aacaggaagg tacggcttaa aaatgcccga accatgcagg
     7921 aatacaattc ggcaacgaat gtgtatttct atgatgctgc tccggacttc aaccgttttg
     7981 ccacaaaaga ctccgaattt gcccgggtga aaataatcag aaatccgcaa ttgcttgtta
     8041 aaatcggcaa aaccgatgtc tcggtaaatg atgtgaaact gattatcaaa ggcttcgaat
     8101 tcgaaccgac aaatccgctg ctgcagcatg caggtaaatt aactccaccg caaaatatcg
     8161 ctgtatccga cagtaacgca actgcctata ccctcagacc cgaatggagc gctgtgcccg
     8221 gtgccgatta ttatgaactg gaattcggcg gaatgctcta ttcaaccatc agggaaaatt
     8281 cgttgctgtt cgaaaacctc actccggaaa ccagatatgc tttcaaagcc cgggctgtta
     8341 acaaaagcgg agcttccgaa tggacaggtt ttgaggctac aaccaaatca gatccgctgc
     8401 agtttgccat cagaggcata aaagctcaga gcaacgtcga aatgcagggc ggagaaggcc
     8461 tcagacgctt gtttgacttt gacgaaggca acatgatgca cacaaaatac ggagaaaatg
     8521 ctgttccgtt tgatttgacc cttgatttga attcggtgaa tcagctcgac aaactcgaat
     8581 acctgccccg cacaagcgga cagaacggca tgtttgaaaa aggaaaaatc ctcttcagca
     8641 acgataagca aacctggatc gaagcgggag aatttgaatg gaaacgaaac ggagaaaata
     8701 aaacgtttgt atttgaaaaa cagccggttg ccaggtatat aaaaatctcg gtagagaaaa
     8761 gtgttggcaa cttcggctca ggcagagaaa tttatatttt caaagttccg ggcaccgaaa
     8821 gctacctgcc gggcgacatc aacaacgata aactcatcga cagcaacgac ctcacttctt
     8881 acctcaacta caccggatta agacttggcg acagcgattt tgaaggctac gtgagccgcg
     8941 gtgatgtaaa taaaaacaac ctgatcgacg cttacgatat ctcaaacgtt gccgtaagga
     9001 tggacgaggc tgaaagtgac gaagataacg gtattgtgga aggtacactc ctcctgactg
     9061 ccgacaaaaa aaactatcgc gaaggtgaaa cggtggaaat aactgtacgt gggcagaatc
     9121 tgaaatcggt gaatgcactc agtttttcac tgccatacaa caacaactat gaattcaccg
     9181 gaatccgaat cctgaatacg ggcgaaatgc agaatttcac caacgaccgc ctccattccg
     9241 atggtgaaaa ggtgctatat cccactttca tcaacgtagg caatcagaaa acactcgaag
     9301 ggaatgcgga tttattcgtt atccgcctga aagccaaacg ggcactgaaa tttgaattaa
     9361 ctccgcaaaa gggattattg gtcggtaaaa atttgagttc gctggctttt taacaaaatg
     9421 cgaaatgtat gaaagcaaga aagaaagtga acatcgggtg tcactttctt tcttgcttta
     9481 atcaaacatc ctgaaaatca tttgaataac atgaaacaaa aatacgggat atttgcggta
     9541 atgaagttga tattttcact gtttttatta taaacccgcc agctcgctga ctttattcca
     9601 gttcaccaca ttccagaatg ctttgacgta agcagcccgt ttggattgat atttcaggta
     9661 ataggcgtgt tcccatacat ccagcgccag gataggtttt cctttcattg tgttgaacgg
     9721 catcagcata ttatcctgat tgggagtaga atcgatgtaa agcgtaccgt ccgatttctg
     9781 catcagccat acccagcctg aaccaaaacg tccggtggcg gctttttcaa attcttccct
     9841 gaatttgtcc acactgccga attgtttcac cagcgctttc tccaacgcag gcgacatggt
     9901 cgttgttccg gccggagcca ttatctccca gaagaaagtg tggttgtaat ggccgccgcc
     9961 gttgtttctc accgcagtct gaatgtcttt gggaagcgta ttgatcttac ggagtagttc
    10021 tgtaatatcc agcttgttgt attgcggata tttctcaaca gccttgttca ggttgtttac
    10081 atacgctgcg tggtgagccg tatggtgaat gcgcatagtg gtactgtcaa taaaaggttc
    10141 cagtgcacca tattcatacg ggagtgacgg aagtttgaac tgtccgaagc taaatacagt
    10201 tatcatcaaa aggaatgata gtaaaatgtt tattcgtttc atagagttgc aatttaaatt
    10261 gattataaat aatttattct tttcagctat aacaatatca tcaaaaaatt gtttaacaga
    10321 ttaaactttc tgttcatcaa acagtcgaat gtataattaa tcacacacaa taatcaattt
    10381 atattactaa gagtggcgac gattttgttg caaacaactc tgataaagta tgttatattt
    10441 aaaaaatgaa aaagtacctg atcctgtcct tttgttttgc catgagtttt gcactccagg
    10501 ctcaacacgc tgtcaaatcg aaaatcataa gtaaatcaac cggtgagccg ctcgaatttg
    10561 tggcagttcg gttactcaat accgactccg cgctggtagc cggacaatca accgactctc
    10621 tcggtgagtt tacccttaaa gacatcaaat cgggcaaata cattctgcag gctacatcgg
    10681 taggctacat cgcaatcaac cggaatcttg agatgagatc ggcaaatgtg gtgctggaaa
    10741 atatccagat ggaagatgac tccaaggtgc tgaaggaact cgaagttgcc ggcacagccg
    10801 tacaagtggt taccaaaggc gataccatcg aatacaacgc aaccacattc aaaaccggac
    10861 aaaatgccgt ggtcgaggat ttgctgaaga aaatgccggg agtagagata acctctgatg
    10921 gaaaaataat ggtcaacgga caggaagtaa agaaaatacg ggtggatggc aagaaatttt
    10981 tcggcgatga tatggaaatg tccaccaaaa atattccggc cgatatggtg gataaagtgc
    11041 aagtggtgga tcagaaatca gaaatgtcac agctcacggg ctttgatgat ggcgaaaccg
    11101 aacgtatcat caacctgacc atcaagcgcg accgccggca gggagtattc ggcaatgtac
    11161 aggcgggagc cggaacggat atcaatccgg aatttcgcta cgatgccaat gcatttctga
    11221 acatcatgaa cggcgatacc cgctcgtcgg tcacagccgg tgccaacaat acaaatactg
    11281 cccgcagccg ccgtggaaga ggcggttttg gcggaggaag aagtggaatc actgctactc
    11341 agaaccttgg gtacaacatc aacacaccgc tgagtaaaca actcatcgta gggggcgacg
    11401 tgacgtttaa ccattcggac aacctggtgc tttcagaaag tcagcgcgaa aactacctgc
    11461 aggacgccac gtaccaaaat aactccaacc ggatgtccaa ccgcgaaaat tatcagggaa
    11521 atctccgcct cgaaatggaa tggaaaccgg acactatgaa ttcttttatc attcagccga
    11581 atataggttt caaccgctcg ttttcacgca gtacgtccga ttatctttat ctgaaagaaa
    11641 acgacagcac tagctgggga aatacaaaaa acagtggcga tggcctcgac cgcgacgccg
    11701 gaattacgtt tatctacaac cgtaaactgg caaaacccgg ccgaacgttt accacacggc
    11761 tcaataccaa tatgtcagca agcgacgaca acggcatgaa ttattcccgg aaaataacca
    11821 acgattctac cattctcatc gaccagcgga gcgacaatac atccaataat ttcaattttg
    11881 gtttgcgact gtcatacgtt gagccgctcg gaacttcgcg caaacatttt ctcgaaacct
    11941 cgctctcctt caacggaaat atccggaact ctgtacgcga tctgtttgat caggatgtca
    12001 acggcaacta catcgtcaag aacaacgaat acagcaacga attttcaaac cgctttttcc
    12061 gtgaaacgct cgacctcaat taccgctacg taatgcctgc atataatctt acgctcggtt
    12121 tttcggccga accatcgcaa acttacagca caactattta cggcgacaac acgggtgttc
    12181 cgattaaaaa tgaagtggtg aacctggctc ctgcagcccg ttttcagtac aatttcggca
    12241 aaagaaattt tgcacggctc gaataccgcg gacgtaccga gcagccctct atcagccaga
    12301 tgcagccggt gaaaaacaac accgacctga tgaacgaaac cgtaggaaat cccgcgctca
    12361 atccggcttt caaccacaat ctccggctca tgtactccaa ctacaatgcc gaaacgttca
    12421 gttcctacag tgtcggagtg ttcggaaacg ccacaaaaga taacctgacg agcaacagca
    12481 tttacgacaa taccggaaaa agatacattc agaccgtcaa ttcgaaagaa ataccgtata
    12541 atctgaatgc ttttttcatg tataatcagc ctttcctgaa gaaattcaat ttctccaaca
    12601 acacttcgtt cggcaccaga caacagtacg gatacacttc caaaaacgta aatgcagccg
    12661 atattaatct taacaacctc ctgatcggcg atttgagcag taccatacgc tacaatgcag
    12721 ccgagaatat ctcgatgagt ttcacccacg aaatagtgga tgtggcactt cgcggcggtc
    12781 tgggctattc ttattcgaaa aataatttca acaaaaaagc atcagaaacc tacgattgga
    12841 caagtgcgct caatttcgga cttcgaccta ccaaaacact gacttttacc accgatctga
    12901 attttacaaa acaaaacgga tacgccgatt tcaacccttc gcagtggata tggaatgcat
    12961 ccttggattt aactgcattc aaggcaaaag gagtgttctc gctgaaagtg tttgatatac
    13021 tgcgtcaaca acagaatatt aatcagaacg taggcgataa ctttgtggaa ttcagcaaat
    13081 caaatgccct gccatcctat ttcctggtta gtttcacata caaaatcaat aagttcaaag
    13141 gcggaaacag tcaggaccgt gaaaatctgg aaaatatgaa ccgcatggga cccggcatgg
    13201 gaaggggaat gagaatggga ggcggcggac cggacggcgg tgcacctcct cctcctccat
    13261 tcatgtaatc cggaaaatca ggaaaccttt tttcattcgt taaacttttc attcagctgc
    13321 ttgttggata agaaaaagac aaaacgggta tgacgaagat aagaattgca tttttaggac
    13381 tgggtggtgt aggcggttat tatggaggaa aactcgctgc acgatacagc ggatcgggta
    13441 gggtggaagt atgttttttt gcccggggcg aacacctgaa agccattcag gaaaccggta
    13501 ttcacataat caccgacgaa gaagacttta tagcccgacc cgatctggct accgataatc
    13561 ctcaggacgc cggtgtggtg gattatgtga ttattaccac caaaagctat gatctccgga
    13621 gttccatcga atttatccgt ccctgtattg gtcctcatac catcatcctg ccattactca
    13681 acggagggga tatcaccgaa cggattcgtg aaattctgcc cgaaaacatc gtctggagcg
    13741 gttgctcata catcgtaagc cgcaaaaccg gaccgggtgt aatcagggca acgggtacgt
    13801 tttacaaact ggttttcgga tacgataagg aaccaaacga acaattgcag aacttcgaaa
    13861 aactccttca ggaggcccgg atagatgcaa agctatccgt caatatccgt gaatccatct
    13921 ggaagaaatt cttcttcatt tcagtttctg cttcacttac ttcctatttt gatgttggtt
    13981 tcaacgaact ggtggatact ccggatcgac tgaacatgac cgtcgggctt gccgaggaat
    14041 tccttaaagt ggctaaagcg gagggaatta atctgggtga aaacgccatt gagcaggtcg
    14101 taaaccgtgc cgaagtgctg cctgccggta ctacaacttc catgcacagc gacttcaaag
    14161 ccggcaaccg cactgaagtg gaaacactta ccggagtcat cgttcagctg gctcagaaac
    14221 accgaatcta cacgccactc tacgaaaccg tatataattc cctgaaaaac aggtgaatag
    14281 tttatagttc tgagttctgt cccgatagct atcgggagag ttctgagtat aacactcgaa
    14341 actcagaact aaactcacgc ttctcccccg aatcccatcg gcagtggcgg catcatgttg
    14401 ttttctggtg tttttatctt tccgtgctgc tgttcatatt tcgaaatgtt gtcctggagt
    14461 gcaaatagca gtcttttggc atgctcggga gtgaggatga tgcgtgattt tacgttggct
    14521 ttgggtgttc cgggcataat gcggataaaa tcaatgacaa attccga
//
LOCUS       DAORKP010000042        14474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_338_length_14474_cov_9.145641, whole genome shotgun sequence.
ACCESSION   DAORKP010000042 DAORKP010000000
VERSION     DAORKP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14474
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_14474_cov_9.145641"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1165
                     /locus_tag="PLN75_03810"
     CDS             437..1165
                     /locus_tag="PLN75_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRG03276.1"
                     /translation="MIHRFSLRNITAIISFLIFSFTAFSLTEVKENPDTYAASAKVEN
                     PGISREKEISHVYDSLHLDKKGLTQPAFDYAISSYTMLKKKGLLDNDSIITIVDFDQP
                     SYQKRMYIIDIKHYKILFNTWTAHGKNTGTVVAKNFSNTPESLKSSLGAFVTEETYIG
                     KHGYSLRLVGLEKCNSSAYQRAIVVHGASYVSQKMINNQGYIGRSFGCPAVPDELSKP
                     IINKIKGGSCFFIYSSTYRPSILI"
     gene            1301..2566
                     /locus_tag="PLN75_03815"
     CDS             1301..2566
                     /locus_tag="PLN75_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRG03277.1"
                     /translation="MPPLAERMRPKNLDEYIGQKHLVGKNSILRLMIDSGNISSFILW
                     GPPGVGKTTLARIIANQLERPFYTLSAVSSGVKDVREVIEKAKSSRFFSQANPILFID
                     EIHRFSKSQQDSLLGAVENGTVTLIGATTENPSFEVITPLLSRCQVYVLKPLEDDDLM
                     ELADRVIKNDTELKKRTIFVDEKEAMLRFSGGDARKLLNIIELVVGSESEVEVHITNE
                     IVTNRLQQNPMAYDKDGEVHYDIISAFIKSIRGSDPDAAIYWLARMVAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANACFDALHKIGWPEGRIILAETTIYLASSPKSNSAYL
                     AIDEALQLVEKTGNLPVPLHLRNSPTKLMKDLNYGKGYKYAHDFDNNFVEQQYLPDKL
                     KKERIWKIQESPAEKKMSEWLRTLWKGRY"
     gene            2578..3450
                     /locus_tag="PLN75_03820"
     CDS             2578..3450
                     /locus_tag="PLN75_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRG03278.1"
                     /translation="MKKLVFTAFALIVFISCSVQKHELQHEPTEMTVMTFNIRLDHAG
                     DKENNWKFRKERVVKSIQFYEADIAGMQEVLHNQLLDLQEKLKEYSYLGVGRADGKEK
                     GEYSPLFYKTKKYAVISSGTFWLSETPDSAGRKGWDAAIERIATWAKFKDKSTGKSFF
                     VLNTHFDHIGSVARLQSGTLIMQKVYELAGKMPVVVMGDFNASPDTPVVKNITNSSNP
                     LHLSDSRAVSPLIYGPEWSFHDFGRTPLEERELIDYIFVKNRVKVLKYGVLAEQDGAG
                     FLSDHCPVLVKVQF"
     gene            complement(3447..4187)
                     /locus_tag="PLN75_03825"
     CDS             complement(3447..4187)
                     /locus_tag="PLN75_03825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRG03279.1"
                     /translation="MIYFISDAHLGSRLVNDPYGHEMRLVRWLDKVKSDAKAIYMLGD
                     MFDFWFEYQTVMPKGFVRFLGKMAELTDSGIEIHYFTGNHDIWTFGYLEKEIGLKIHR
                     KAEITELSGKKFYLAHGDELFSDEKGFRIIRRIFHSPTLQKLFQLVPPRIGQNFGYAW
                     SKSNREKILHLENNFQGEDNEPLVVFAKKFAETNDVDFLIFGHRHIELDLELKNKSRV
                     IILGDFIGIFSYGIFDGENFRLETDLLA"
     gene            complement(4233..4547)
                     /locus_tag="PLN75_03830"
     CDS             complement(4233..4547)
                     /locus_tag="PLN75_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRG03280.1"
                     /translation="MNEFLEKEERIVRMLRTVFDPEIPVNIYDLGLIYRIDVKDDGVV
                     DIDMTLTAPNCPAVDFIVEDVIMKVESVEGITKANVNIVFDPPWDKDMMSEEAQLELG
                     FL"
     gene            complement(4600..5001)
                     /locus_tag="PLN75_03835"
     CDS             complement(4600..5001)
                     /locus_tag="PLN75_03835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRG03281.1"
                     /translation="MYIYELDFKVRDYECDLQGIVNNSVYQNYLEHTRHEFLLSRNVS
                     FSGLHEQGVDAVVARIEIAFKNSLRSRDEFVCKLNVKKEGVKYVFHQAIYRKPDNKLC
                     ITAKVTTVVVVDGKLVSQFGIFDELVAESEQ"
     gene            complement(5046..5672)
                     /locus_tag="PLN75_03840"
     CDS             complement(5046..5672)
                     /locus_tag="PLN75_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRG03282.1"
                     /translation="MTIEEYILNHIDEEPAYLAAINRNTWVRQMNPRMCSGHLQGRVL
                     SMIARMIQPQQILEVGTFTGYSALCFAEALPENGCVDTIEMDDELEDFIRDNCRNSPY
                     ENKINLYIGDALEIIPSLEKMYDLVFLDAEKSQYTRYYDAVFPKVKNGGYILADNTIW
                     DGKVTQNVLSNDLKTREIIRFNDLIKADSRVEKVILPLRDGLTIIRKK"
     gene            complement(5679..6902)
                     /locus_tag="PLN75_03845"
     CDS             complement(5679..6902)
                     /locus_tag="PLN75_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRG03283.1"
                     /translation="MDNYNTYQLDNGLRIIHLPQSSPVSYCGVIVNVGSRDETGDQQG
                     MAHFIEHMLFKGTGKRRSAQIINRLEDVGGELNAYTSKEETVIYAGFLSEYTERTIEL
                     IADLIQHSTFPEAEVEKELVVIQDEIKSYKDIPAELIYDDFEEMLFDSNPMAHNVLGK
                     NENLETFTAKDALEFYHTFYQPENMVFFSVGNTDLKKIIRWVNRYFNFQKNGFADHVR
                     TAPQIPVKQHKIVSRDTHQVHCLMGARSYDIYHPERMTMYLLNNILGGPGMNSLLNLS
                     LRERNGLVYSVESTFQPFTDCGWWGIYFGTDPENAVKCERLVQRELTKLCENKISESK
                     LKKYKLQLMGQLAIAAENKENIALSLGKSYMRYGKFDNNEQIRKKVEAITSERLLSVA
                     QEMFDVKNLTLLKYT"
     gene            complement(7002..8594)
                     /locus_tag="PLN75_03850"
     CDS             complement(7002..8594)
                     /locus_tag="PLN75_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03284.1"
                     /translation="MKKGMILTVFVALFTMLSASNKTAIDYYESGDMKKAKSLFLASS
                     KLDAVDYYYLGMINLKENNKNAASESFNKGLQADPANLFNSVGLAAIQLASDPKTAEK
                     ALKAISGNKLYKKDPKMQVAIAEAFALIKNKALTDMFLAKAKKADKKSALPYIMEGNQ
                     LMAQGKTNEAAVSFENAVYFDPNSKIALVKLAQLYVGTRKQIAFDYLDKTTTLDPGYE
                     YGWKTQADLRYKNGFYPEARKAFEKYMSLIKPQPDDYQTYGEILYFNKDYPASLEALA
                     KAPVNTVTNRLKMYSLHDLNKFDEAIPIAETLVSTTAASDLIYQDYAFYADMLNKTKN
                     YQGAAKYYELAYKADTTRISGLTDIARAYERAKDYPNAVKYYQKVIDVNPAHTLADMY
                     SLGVAYYSAGTDLTATPDEMKRLENLSKASDAFGKMATTFPDQYVGLLYQARANSAMD
                     PETTKGLAKPYYEKVLPLLLLTPTERKAEIMEVYQYMGIYFLKKDDYPQSRAYWVKVL
                     ELDPQNAIAKQVIASIDSVKKK"
     gene            complement(8687..9628)
                     /locus_tag="PLN75_03855"
     CDS             complement(8687..9628)
                     /locus_tag="PLN75_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRG03285.1"
                     /translation="MKKEILLPVLLLSFMMMMACGRKAKNSENANTMTSGYIKIAVDQ
                     TVQNVIHNEIEVFQNLYPVKIEPIYTSEQEAIELLKKDSVRLAITARKLSQAELSYFH
                     EKTFQPEMIRIAIDGVAIITHPSNKDSVISVKDMKRILTGEVKQWNQIYPNSKLGKIQ
                     VVFDNTNSSIVRYANDSICRDKPLSRDLSALELNEQVVEHVAKVPNALGLIGVNHISD
                     MNDSLVVDFTSKIRVMKVSYDDNANADNSVQPLQYYLYNAEYPFRREIFILLNDPRGE
                     LPKGFTRFIVGDKGQRIIKRTGLLPSTMPIQAVKIVN"
     gene            complement(9840..10673)
                     /locus_tag="PLN75_03860"
     CDS             complement(9840..10673)
                     /locus_tag="PLN75_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRG03286.1"
                     /translation="MAKDINLTSEKWIDLIFEGRNKNYGAYTLRKNSPKRHTFAFLAV
                     VAAAFLIVLASIGITQYNKAKEAREAMTQVSELSDIKLNKPDVPEENVVKKFEAPPPV
                     ELKSTIQFTAPIIKKDEEVPDEQTMKSQEELVKSDVQISIADVKGTNEETGVDIATLQ
                     EHKVIVQEEVVEEKVFEIVEQPPSFPGGEAAMYEWLSKNINYPVIAQENNIQGRVTCQ
                     FVVGRNGEIEDVRVVRGVDASLDKEAIRVIRSMPKWIPGKQGGNAVKVRYTLPVQFKL
                     Q"
     gene            complement(10717..11343)
                     /locus_tag="PLN75_03865"
     CDS             complement(10717..11343)
                     /locus_tag="PLN75_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRG03287.1"
                     /translation="MQVDSGGSGKKGERKKMDTRVDFTPMVDMIMLLLTFFMLATTLS
                     KPQTMEIALPSKEKGDEKNQTLAKASEAVTLYLGADNKVYYFMGEPDLTTPNFLKSTT
                     YAADGLRALILNKNRKAVTQVNELKDKKLRLQITQEQYDKELSKIKVQDGTPTVIIKP
                     LDNSNYDNLVSALDEMLITSVGKYAITEVDENDKKMLTNSGVDFAVKK"
     gene            complement(11437..12066)
                     /locus_tag="PLN75_03870"
     CDS             complement(11437..12066)
                     /locus_tag="PLN75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRG03288.1"
                     /translation="MPKIKVKRKDTWIDMTPMSDVMVLLLTFFMLTSTFLKPEPVTVA
                     TPLSVSEIKIPEKNVMSVLISPTGQVFLDIDNPNDRIAALEKMASAYNIQLTDKEKYE
                     FSIATSFGFPMQSFKQFMALPKEKRNEELPKLGIPKDSTDNQFKGWVLAAREVKGKDM
                     VIAIKADSETPYTSIKSVMGSLQDLKENRYNLITSLREVETTPAATAAK"
     gene            complement(12165..12983)
                     /locus_tag="PLN75_03875"
     CDS             complement(12165..12983)
                     /locus_tag="PLN75_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRG03289.1"
                     /translation="MAKEKQPTKTKKQSKGLSAALVIVLSFGVALLIYNFVFGNPSNF
                     IDNNPDNHPLQGNMLGTIYKGGFVVPILLTWLLSVIILSVERFIALNRSRGTGNLDTF
                     VMSVKQKLEAEDIQGAKELCAKQKGSVAAVVDAGLRTYEQMANDTTGLLKEQKLAAIG
                     TMIEQATALEMPTMNQNMPVIATFTNLGVLTGLFGTVMGMIRAFSSLGGGGGVDSAAL
                     SVGISEALVNTAFGILTGALAVIAYNYFSAKIDNITYAIDEIGFAITGVFQSKH"
     gene            13640..>14474
                     /locus_tag="PLN75_03880"
     CDS             13640..>14474
                     /locus_tag="PLN75_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRG03290.1"
                     /translation="MDYNFREIEQKWQKFWIENRTYKTEIDHTKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLNGFNVLHPMGYDAYGLPAEQYAIQTGQHPAVTTQK
                     NIKRYREQLDKIGFSYDWDREIRTCEPDYYKWTQWAFIQMFNSYYSNQTQQARPIAEL
                     VEVFAEQGTAGLNIACTEELSFTASEWNAKTEKEQQEILLNYRIAYLADMKVNWCAAL
                     GTVLANDEVSEGFSIRGGHPVEQKVMRQWSLRVSAYAQRLLDGLDKIDWTDSLKETQR
                     NW"
BASE COUNT         4139 a         3011 c         2773 g         4551 t
ORIGIN      
        1 cacaaaggat aaaaatctta gatttttata aattatgact tttcggagtg gactcctaaa
       61 tgctaaaaat aaattactta ttgaagggga agtaattaca aaacgtttac ctatcttact
      121 aagacttata aaaaaaataa ctattgtccg gttaaaattc tcagatccat cgctacgctc
      181 cggctgcctc aggcagggat gatagtgatt taacccacac ctaaaagata aaatgtttaa
      241 aacgatgaga caaataaacc tacaatcaac tcattatcaa catttaacaa gtttactaaa
      301 tatttttata aattttaccc tatatcgtta aagttattta ttatttgagc cgttccggga
      361 acgttacaat ttattttgtc gaaatttgca cattattaat tcataaaact gcccggtccg
      421 ttaattttca ttttctatga ttcaccgctt ttcgctgaga aatatcacag caattatttc
      481 atttcttatc ttttcattta cagccttttc actcaccgaa gttaaagaaa atcctgacac
      541 atatgctgcc tcagcgaaag ttgagaaccc gggtatttcc cgtgaaaaag aaatttctca
      601 cgtgtatgac agcctgcatc tcgacaaaaa ggggcttacg cagcctgcat ttgactatgc
      661 aataagcagt tacacgatgt tgaagaaaaa aggattgctg gacaacgatt ccataatcac
      721 cattgttgat tttgatcagc ccagttacca gaaacgaatg tatatcattg atattaagca
      781 ctataaaata cttttcaaca cctggacagc tcacggaaaa aacacaggca cagtcgtagc
      841 caaaaatttc agcaacaccc ccgaatcact taaaagcagt ctgggagcat ttgttacgga
      901 agaaacctat atcgggaaac acggctactc gcttcggctg gtgggactcg aaaaatgcaa
      961 ctcaagcgcc taccaacgag ctatcgttgt tcatggagcc agctatgtaa gccagaaaat
     1021 gattaacaat cagggttata tcggccggag cttcggatgt cccgctgtac ccgacgaact
     1081 ctcaaaaccc atcatcaaca agatcaaggg tggctcctgc ttttttatct acagcagcac
     1141 ttatcgccca tcgattttga tctgaaaact tcaaaaaaag gatacaaaat taaggttgga
     1201 cactcgttca accttttttt ttcaaaaaaa gcaatccgct ttatttcttt tttatcctga
     1261 aaaaaaatca taactttgtg tattcattaa ccaaacgaat atgccccctt tagcagaacg
     1321 gatgcgtccg aagaacctgg acgaatacat tggtcagaaa cacctggtcg ggaaaaactc
     1381 cattctacgg ctgatgattg actcggggaa tatttcatca tttatactct ggggacctcc
     1441 cggagtaggc aaaaccaccc ttgcacgcat tattgccaat cagttggaaa ggccatttta
     1501 taccctcagc gctgtgtctt cgggggtaaa agatgtccgt gaggtaattg aaaaagccaa
     1561 atcgagccgt tttttctctc aggcaaatcc tatcctgttt attgatgaaa ttcaccgttt
     1621 cagcaaatca cagcaagatt cactgctcgg agccgttgaa aacggcactg tcacgctgat
     1681 tggcgccacc accgaaaatc cttcgttcga agtaatcact ccgttgctat cacgttgcca
     1741 ggtgtatgta ctgaaaccgc tggaggatga tgatttgatg gaactggccg acagagtaat
     1801 caaaaacgac actgaactga agaaaagaac catttttgtc gatgagaaag aagctatgct
     1861 ccgattctcg ggtggagatg cccgtaaact gctcaatatt attgaattag tagttggatc
     1921 cgaatctgaa gttgaagttc atatcaccaa cgaaatagta acaaaccgac ttcagcaaaa
     1981 cccgatggct tacgataagg acggtgaggt gcattacgac ataatttcag ctttcatcaa
     2041 atcgattcgt ggcagcgatc ccgatgctgc catatactgg cttgcacgca tggtggccgg
     2101 aggcgaagac ccgaagttta ttgcccgccg actggtgatt tctgctgctg aagacatcgg
     2161 actggcaaat ccgaacgctc tgctcctggc caatgcatgt tttgatgcat tgcataaaat
     2221 cggctggccc gaaggacgca ttatcttagc cgaaaccacc atctacctgg cttcatctcc
     2281 taaaagcaat tcggcctatc tggctattga tgaggctctt cagttggttg aaaaaacggg
     2341 caatcttccc gttccgctcc atttgcgcaa ttcacctaca aagcttatga aagatttgaa
     2401 ttacggaaaa gggtataaat atgcgcatga tttcgacaat aattttgtgg aacaacaata
     2461 cctgcccgat aaacttaaaa aagaacgaat ctggaaaata caggaaagtc cggctgaaaa
     2521 gaaaatgagc gaatggctca ggacactctg gaaaggaaga tattaaatca tttgattatg
     2581 aagaaattag tatttacggc attcgcatta atcgttttca tttcctgctc agttcagaaa
     2641 catgaactgc agcatgaacc caccgaaatg actgtaatga cttttaacat caggcttgat
     2701 cacgcgggtg acaaagaaaa taactggaaa ttcagaaaag aacgggtagt aaaatcaatc
     2761 caattttacg aagctgacat tgcaggcatg caagaggtgc tccacaacca gcttttggat
     2821 ttgcaggaga aattaaaaga atacagctac ctgggagtgg gcagagccga cggcaaagaa
     2881 aaaggagaat acagtccctt gttttacaaa acaaaaaaat acgctgtgat ttcatcgggc
     2941 acattctggc tgagcgaaac acccgattct gcaggacgca aaggatggga tgcagcaatt
     3001 gaacgaatag caacgtgggc taaattcaaa gataaatcaa ccggaaaatc cttttttgtt
     3061 ctgaacacgc attttgatca cataggcagc gtggcacgac tccaaagtgg cacactgatt
     3121 atgcaaaaag tatatgaact tgccgggaaa atgcctgtag tggtgatggg cgacttcaat
     3181 gcttcgccgg atactccggt ggtgaaaaat attaccaatt catccaatcc tcttcactta
     3241 tcagattcgc gtgctgtttc accgttgata tatggtcctg agtggtcgtt tcatgatttc
     3301 ggacgcactc ctctggagga aagagaactg attgattata tttttgtaaa aaacagggtg
     3361 aaagtactta aatatggtgt actggcagag caggatggag caggttttct gtcggatcat
     3421 tgtccggtac tggtgaaagt tcagttctag gcgagtaaat ctgtttccaa ccgaaaattt
     3481 tctccgtcga aaatgccgta agagaaaatg ccgataaaat ctccaagaat aattacgcgt
     3541 gatttgtttt tcagttccag atcaagttca atgtgcctgt gtccgaaaat caggaaatcc
     3601 acatcgttag tttccgcaaa ttttttggca aaaaccacca gcggctcatt atcttcgccc
     3661 tgaaaattat tttcgagatg gaggattttc tcacggttac ttttagacca ggcatatccg
     3721 aaattctgcc cgatacgtgg tggaaccagc tgaaacaact tctgaagggt gggactgtga
     3781 aaaattctcc ggataatccg gaatcctttc tcatcagaaa ataactcatc gccgtgtgcc
     3841 aagtagaatt tcttaccgct tagttccgta atttcggcct tacggtgaat tttcaatcca
     3901 atctccttct ccaaatatcc aaacgtccaa atatcatggt tgccggtgaa ataatgtatt
     3961 tcgataccac tatcggtcag ctcggccatt ttccctaaaa aacggacaaa acctttaggc
     4021 atgactgttt ggtactcaaa ccaaaaatca aacatatcac ccagcatata aatggctttg
     4081 gcatcggatt ttaccttatc aagccacctg accagacgca tttcgtgccc gtacggatca
     4141 ttcaccagac gggaacccag gtgtgcatcg gaaataaagt aaatcatttg cgggtaattg
     4201 gttatacggt tgagtggttg ataggttcaa attcagagaa accccagttc gagctgagct
     4261 tcctcactca tcatatcttt atcccagggc gggtcaaaaa cgatgtttac attagctttg
     4321 gtaatacctt ccacgctttc cactttcatt atcacgtcct caacaataaa atccacagca
     4381 ggacaattgg gggcagtgag tgtcatatca atatccacta ctccatcatc ttttacatca
     4441 attctgtaaa tcaatccgag gtcgtaaata ttaacgggta tttccggatc gaaaacggtg
     4501 cgaagcatcc gaactattct ttcttctttt tctaaaaatt cattcatatt cgttctgttc
     4561 aattaaccat caaacaaatt aatgcctcaa aaagttgatt cattgctcac tttctgccac
     4621 taattcatcg aaaataccga attgtgacac cagttttcca tccactacca ctacggttgt
     4681 aactttagct gttatacaca gcttgttgtc cggtttacgg tagattgcct gatgaaaaac
     4741 atattttaca ccctcttttt tgacgttgag tttgcaaaca aactcatcgc gtgagcgaag
     4801 cgaatttttg aaagcaattt ctatgcgcgc cactacagca tcaactccct gttcgtgcaa
     4861 cccggagaaa ctcacatttc tgctcagcaa aaattcgtgc cgtgtgtgct ccaggtaatt
     4921 ctgatacacc gagttattca caattccctg taaatcacac tcataatcgc ggactttgaa
     4981 atccagttcg taaatataca ttttgtatgc ctgttaaatt tattcgatga atttgattct
     5041 tcaaattact ttttgcggat gattgtcaag ccatcccgaa gcggcaaaat cactttttcc
     5101 accctgctat cagcttttat caggtcgttg aaacggataa tttcacgggt tttcaggtcg
     5161 tttgagagca cattttgagt cactttcccg tcccagattg tattgtcggc caggatatat
     5221 cctccgtttt ttactttggg gaataccgcg tcataatacc gtgtgtactg gcttttctcc
     5281 gcatccagga aaaccagatc gtacattttt tccaacgaag gtattatctc gagggcatcg
     5341 ccaatgtaca ggtttatctt gttttcgtag ggtgaatttc tgcaattatc acgaataaaa
     5401 tcctccagtt cgtcatccat ttctattgta tccacacatc cgttttcagg aagggcttcg
     5461 gcaaagcaaa gcgcagagta gcctgtaaaa gttccaactt caagtatctg ctgcggctgt
     5521 atcatccggg caatcatgct cagcactcgt ccctgcaaat ggcccgagca catccgcgga
     5581 ttcatctgtc tgacccatgt attgcggttg atagcagcca gataagcggg ttcttcatca
     5641 atatgattca ggatgtattc ttcgatggtc atcgcgtgtc aggtatattt cagtaaagtc
     5701 aggtttttca catcaaacat ctcctgagct acgctgagca atcgttcaga agtgattgcc
     5761 tccactttct ttctgatttg ttcgttgttg tcaaattttc catatcgcat ataacttttc
     5821 cctaaagaga gcgcaatatt ttccttgttt tcggctgcaa ttgccagctg acccatcagc
     5881 tgaagtttgt atttcttgag tttagattct gaaattttgt tttcacacaa tttagtcaat
     5941 tcgcgctgaa ccagtcgctc gcatttcaca gcgttttcag gatctgtacc aaaatatatt
     6001 ccccaccagc cacaatcggt aaacggttgg aatgtagatt ccacagaata tactaatccg
     6061 tttcgttctc tgagtgaaag attaagcaag ctgttcatgc ccggtccacc aagaatattg
     6121 ttcagcagat acatggtcat tctttcggga tgataaatat cataggatct cgctcccatg
     6181 aggcaatgaa cctgatgtgt atcccggctg actattttat gttgtttaac cggaatctgc
     6241 ggagcagttc ggacatgatc agcaaaccca tttttttgaa aattaaaata cctgttcacc
     6301 catcggatga tttttttcaa atcagtgttt cccaccgaaa agaataccat attctccggc
     6361 tgatagaaag tgtgataaaa ttcgagtgca tctttggctg tgaaagtttc gagattttcg
     6421 tttttgccca acacattgtg cgccatcgga ttggagtcaa aaagcatttc ttcgaaatcg
     6481 tcgtaaatca actcagctgg tatatctttg taagatttta tttcatcctg tattaccacg
     6541 agttctttct ctacttccgc ttcaggaaat gtggaatgtt gtatcagatc ggctatcagt
     6601 tcaatggttc gctcggtata ctcactcaga aagccggcgt aaattaccgt ttcttcctta
     6661 gaagtgtagg cattgagttc tcctcccaca tcttcgagtc tgttaatgat ctgtgctgag
     6721 cgtctcttgc ctgttccttt aaaaagcatg tgctcaataa aatgtgccat gccctgttga
     6781 tccccggttt catctcttga tcctacattg acaatcactc cgcagtacga caccggcgac
     6841 gactgaggta aatgaattat tctcaaaccg ttatccaact gatatgtatt gtaattatcc
     6901 aaaccttagc tataaaaaat tgcgtgcaaa gttaatactt tttttttgac gtaaaaaaaa
     6961 gactgcccga aagcagtctt ttgaataatt ggttttttag attatttttt cttaacggaa
     7021 tcgatgcttg caatcacttg cttagcgatt gcattttgcg gatcaagttc gagaactttc
     7081 acccaataag cgcgtgattg aggataatcg tcttttttca ggaaataaat acccatatac
     7141 tgatacactt ccatgatttc agctttacgt tcagtcggag tcagaagcaa cagaggcagc
     7201 actttttcat aatagggttt tgcaagtcct ttagtggttt cgggatccat ggccgaattt
     7261 gcacgagcct gatatagcag tccaacgtat tgatccggga aagtggtagc cattttgccg
     7321 aaggcgtcgc ttgctttgga caagttctca agacgtttca tttcatcggg agtagctgtg
     7381 agatctgttc ctgcactata atacgccaca cccaacgaat acatatctgc cagtgtgtga
     7441 gccggattta cgtcaattac tttctgataa tattttacag catttggata atctttggct
     7501 cgttcatatg cacgtgctat gtcggtaagt ccactaatgc gagttgtatc agccttgtat
     7561 gctaattcat aatactttgc tgcaccctga taattttttg ttttgttgag catatcggca
     7621 tagaaagcat aatcctgata gattaaatca gatgctgcag tagtgctgac aagtgtttcg
     7681 gcaattggaa tagcttcgtc gaatttattc aggtcatgca gactgtacat tttaagccta
     7741 ttggtaacgg tatttacagg ggctttggcc aacgcttcca gtgacgcagg ataatctttg
     7801 ttaaaataaa gtatttcacc ataggtctga taatcatccg gttgaggttt gatcagactc
     7861 atgtattttt cgaaagcttt gcgggcttcg gggtaaaaac catttttgta acgcagatcg
     7921 gcctgtgttt tccatccgta ttcgtagccc ggatcaagtg tggttgtttt atccaggtag
     7981 tcgaaagcta tttgtttgcg ggtaccgaca tacaattgtg ccagttttac cagggcaatt
     8041 tttgaattcg ggtcaaagta aaccgcattt tcaaaactta cagcagcttc gttagttttc
     8101 ccctgagcca tcagttggtt gccttccata atatagggaa gcgcactttt cttatcggct
     8161 tttttggctt tagccagaaa catgtctgta agtgctttat ttttaatcaa ggcaaatgct
     8221 tcagcaattg caacctgcat tttcggatct ttcttataca gtttatttcc actgattgct
     8281 ttgagtgctt tttcagctgt tttggggtcg gaggcgagtt gtattgctgc cagacccaca
     8341 ctattgaaaa gattggccgg atctgcctgt aatcctttgt taaaagattc acttgctgca
     8401 tttttattgt tttctttgag atttatcata cccagataat aataatctac cgcatcaagt
     8461 tttgaagagg ctaaaaacag agacttggct tttttcatat ctccggattc gtaataatca
     8521 atcgctgttt tattgcttgc tgacaacatg gtgaaaagtg ccacaaacac tgttaaaatc
     8581 attccttttt tcatttttct ttaatataat tgttagtatt tatttcaatt taatgtatat
     8641 tacccaaatt ttgtaccaaa tcttctgttt gtttcaaatt ttgagtttaa tttacaattt
     8701 tgacggcttg aatgggcata gtggaaggta aaagtccggt acgtttgatg atacgctgcc
     8761 ctttatctcc tacaatgaaa cgggtgaatc ctttgggcaa ttcgccccgc gggtcattca
     8821 aaagtataaa aatttcacgt ctgaaaggat attctgcgtt atacaaataa tactgaagag
     8881 gctgtacact gttatcagca ttagcattat catcgtatga aaccttcata acgcgtattt
     8941 tagaggtaaa atccactacc agcgaatcat tcatatcgct gatatgattc acacctatca
     9001 agcccagtgc attgggaacc ttagccacat gttctactac ctgctcgttg agttccaatg
     9061 cgctcaaatc acgtgataga ggtttatccc tgcagattga atcgttggca tagcgcacga
     9121 tgcttgaatt ggtattgtca aaaacaacct gaattttgcc gagctttgaa ttaggatata
     9181 tctgattcca ctgcttcact tcaccggtga gtatgcgctt catatcctta acacttatta
     9241 cggagtcttt gtttgatggg tgcgtaataa ttgcaacacc atcaatagct attcgtatca
     9301 tttcgggttg aaatgttttt tcatgaaaat agctgagttc agcctgagat aatttgcggg
     9361 cagtgatggc taaacgtaca ctgtcttttt tcagaagctc tattgcttcc tgctcggatg
     9421 tgtaaatagg ctctatctta acaggataaa gattctgaaa aacttcaatt tcgttgtgaa
     9481 taacattctg tacagtctga tcaacggcta ttttgatata tccgcttgtc atcgtattgg
     9541 cattctcact gtttttggcc tttctgccac aagccatcat catcataaac gataatagca
     9601 ataccggaag caggatttct tttttcatat tttattttaa aaattaatca ggtgtaggct
     9661 ttgagtcttt tttgaaattt acattttaat gtattaacat agttaatttc gggagaatca
     9721 ggatcaacaa taataattca actgtaattt catttgtgtt aaacaagaca aaatttaatc
     9781 aaatcctgaa aaacatcaag actgattaaa ttttgtcatt ttaattgttt ttttaagtat
     9841 tattgcaact taaactgaac aggtaaagtg tatcgtactt tcacagcgtt tccaccttgt
     9901 tttccaggaa tccattttgg catggatctg atcacacgga tagcttcttt gtccaagctg
     9961 gcgtcaaccc cgcgaactac tcttacgtct tcaatttcac cattacgccc tacaacaaac
    10021 tgacacgtta cacgtccctg aatgttgttt tcctgcgcaa ttacaggata gttgatgttt
    10081 ttgctgagcc attcgtacat ggctgcttca cctccgggga acgaaggcgg ttgctccacg
    10141 atttcgaata ctttttcttc cactacttct tcctgtacga tgactttgtg ctcctgaagt
    10201 gtagctatat ccacacctgt ctcctcattg gtacccttta cgtcggctat tgatatctga
    10261 acgtcggatt ttaccagttc ttcctgcgat ttcattgtct gttcgtcagg tacttcctcg
    10321 tcttttttaa ttatgggagc agtaaactga atcgtactct tcaactctac aggaggtggt
    10381 gcttcaaatt ttttcaccac attttcttcg ggtacatcgg gcttattgag cttaatatct
    10441 gacaattcag aaacctgagt cattgcttct cttgcttctt ttgctttatt gtattgagta
    10501 atcccaatgg aagccaatac aattaagaat gcagcagcta caacagcaag aaatgcaaaa
    10561 gtgtgtcttt ttggagaatt tttacgcagt gtataagctc cgtaattttt atttctacct
    10621 tcgaagatca aatcaatcca cttctcagat gttaaattta tgtcttttgc cattatttat
    10681 tagtttttta aactgttagt tttaactgga gaatcactac tttttaactg caaaatccac
    10741 accagaattt gtgagcattt ttttgtcatt ctcatctacc tcggtaattg catatttacc
    10801 tacgcttgtt atcagcattt cgtcgagagc cgaaaccagg ttatcgtagt tggaattatc
    10861 aagcggtttg atgattaccg taggagttcc atcctgtacc tttattttac tcagctcctt
    10921 atcatattgc tcttgagtaa tctgcaacct caattttttg tctttcaatt catttacctg
    10981 cgttacggct ttgcggtttt tgttgagtat aagcgctctc aagccatcgg cagcataggt
    11041 agttgatttc agaaagttgg gtgttgtcag atcgggctct cccatgaaat aatacacctt
    11101 gttgtctgct cccagataga gggttacagc ttcagaagcc ttcgccaggg tttggttttt
    11161 ttcatcccct ttttcttttg aaggcaaggc tatttccatg gtttgaggtt tactcaaagt
    11221 cgtggcaagc ataaagaaag taaggagcaa cataatcata tccaccatgg gtgtaaagtc
    11281 caccctggtg tccatttttt tacgttcgcc tttttttccg ctgccgccgg aatcaacttg
    11341 catcatatct ttataaagtt ttaatagtta ttatcttttt tgctccggat tacttaaaaa
    11401 aaaacagatg aaccggagta aaaagtgttt tatttgttat ttggctgctg ttgcagctgg
    11461 cgttgtttca acttcacgca gtgaagtgat caaattgtac cggttttctt ttaagtcctg
    11521 cagagaaccc atcacacttt taatggaggt ataaggagtt tcggagtcgg ctttgatagc
    11581 tatcaccata tcttttcctt ttacttcgcg tgcagccaat acccatcctt tgaactgatt
    11641 atcggtcgaa tctttaggta tcccgagttt tggcaattct tcgttccttt tttctttggg
    11701 aagtgccata aattgcttga aactttgcat tgggaaaccg aaggatgtag ctatactgaa
    11761 ttcgtatttt tccttatcgg taagctggat attgtaagcg gatgccattt tctcaagagc
    11821 tgcaattctg tcgtttggat tatcgatatc caaaaacacc tgaccagtag gagaaatcaa
    11881 cactgacatt acgttctttt caggaatttt aatttctgaa acggacaacg gtgtggcaac
    11941 tgttacgggt tcaggcttca ggaacgtaga agtcaacatg aagaatgtga gcagcagtac
    12001 cattacgtcg ctcatggggg tcatgtcaat ccacgtatct ttccttttaa ctttaatctt
    12061 tggcattttg tctttcttta aattaattat atgaatcaat cataaatatt atagtaatct
    12121 aattttcact taaattactc tggtctgtta caatcagatt ctaattaatg cttagattgg
    12181 aaaacacctg tgattgcaaa tccgatttcg tcaatagcgt aagtaatgtt atcaattttt
    12241 gcagagaagt agttgtaagc aattacagcc aacgctccgg tcaagatacc gaatgctgta
    12301 tttacaagag cttcagaaat acctacagaa agtgcagctg agtcaacacc accgccacct
    12361 ccaagagagg agaacgcacg gatcataccc ataacggttc cgaacagccc tgtcaacaca
    12421 cctaagttgg taaatgtagc aattacaggc atgttctggt tcatagtagg catttcaagt
    12481 gcagtagcct gttctatcat tgtaccgata gcagctaatt tttgttcttt caaaagtccg
    12541 gtggtatcat ttgccatttg ttcgtaagtt cttaaacctg catctacaac agctgcaaca
    12601 gaaccttttt gttttgcaca aagttctttt gcaccctgaa tgtcttcagc ttccagtttt
    12661 tgttttacac tcattacgaa ggtgtcaaga ttaccggttc cgcgacttct gttcaaagcg
    12721 atgaaacgtt caacacttaa aatgataact gacaataacc atgtaagaag aatcggtaca
    12781 acaaaacctc ctttgtaaat tgtacctaac atgttaccct ggagtggatg gttatccgga
    12841 ttgttgtcaa taaagttgct tggattacca aacacaaaat tgtaaatcag aagtgcaaca
    12901 ccaaaactta gcacgattac taacgcagca gacagtcctt tggattgctt tttggtcttc
    12961 gttggttgtt tttctttagc cataatagta atttttgtgt aacattttgt tgaatgttac
    13021 gtagttaata atttagaaat tttaattgtt ttaattttat ttgaattttc ttttttttta
    13081 ttcaacgtca gatattgatt attattttct tattagcttt tgaacgtaca aaaataatat
    13141 attttaggta attaatgcaa taaacggaaa atatttttca atttaattaa tcaaaaacac
    13201 aaaaaaacac agcattaaat atcacttcat tttttattta tttcgttttg tgtttatttt
    13261 tttcctgatc aaaggggaat atcccggttt cttttcgtac tataaagtct aaaaaatcag
    13321 gagaaacaaa ccatttggta cttatgaaat tacataaggt gaaacataat catttcaact
    13381 tttttatcat tgactgtgcc tgtccttgca attgaccaac taagtaaaaa tgcaaattta
    13441 tgaaaaaaat ctacattttg tctggtttcg gttttttatt taagaactta aagaaaacaa
    13501 gaaacaatgg tactcatagt ccggattttc ctatgcaata tgaacattat ttttttattt
    13561 tcacttgaat ttcgtacctt tgcacactat taaaaacata ggggataaat caaatcatta
    13621 atctcattat caattattta tggattacaa tttcagagaa atagagcaaa aatggcaaaa
    13681 attctggatt gaaaacagaa cctacaaaac cgaaatcgat catacaaaac ccaaattcta
    13741 cgttttggac atgtttccgt atccttcagg tgccggactg cacgtagggc atccgcttgg
    13801 ctatattgct tctgatattt acagccgcta caaaaggctg aatggcttca atgtgcttca
    13861 tccgatggga tatgatgcct acgggcttcc ggctgagcaa tatgccatcc agacaggcca
    13921 gcatccggcc gtgacaactc aaaaaaacat aaaacgctac cgcgaacaac tggataaaat
    13981 cggtttttct tacgactggg accgtgaaat acgcacttgc gaaccagatt actataaatg
    14041 gacacaatgg gcatttattc agatgtttaa ttcgtattac agcaaccaga cgcagcaggc
    14101 gcgtccgatt gccgaattgg ttgaagtatt tgcagagcag ggaacagctg gtctgaatat
    14161 tgcctgtact gaagaattga gttttactgc ctctgaatgg aacgccaaaa ccgaaaaaga
    14221 acagcaggaa atcctgttga attaccgcat tgcctactta gcagatatga aggtgaactg
    14281 gtgtgcagca ctcggcaccg tactggccaa cgacgaagtg agtgaaggat tttcaatccg
    14341 cggtggtcat ccggtagaac agaaagtgat gcgccagtgg agcctgcgtg tgtctgctta
    14401 tgctcagcgg ctacttgacg gcctcgataa aatagactgg acagattccc tgaaagaaac
    14461 acagagaaac tgga
//
LOCUS       DAORKP010000043        14390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_344_length_14390_cov_8.432996, whole genome shotgun sequence.
ACCESSION   DAORKP010000043 DAORKP010000000
VERSION     DAORKP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_14390_cov_8.432996"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..845
                     /locus_tag="PLN75_03885"
     CDS             105..845
                     /locus_tag="PLN75_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRG03291.1"
                     /translation="MPSKLVLIPTYNEKENIENIIRAVFRLAGEFHILVIDDGSPDGT
                     AGIVHRLMSEFPNSLFILEREGKLGLGTAYIAGFRWALARGYDYIFEMDADFSHNPED
                     LPRLYNACNDEGADVAIGSRYISGVNVVNWPIGRVLMSYFASKYVRLITGLKIADTTA
                     GFKCYRRQVLETIELDKIRFKGYAFQIEMKFTAYKCGFTLKEVPIIFINRRLGTSKMS
                     GGIFGEAFSGVFKMKISSFSRKYPQKKA"
     gene            902..2245
                     /locus_tag="PLN75_03890"
     CDS             902..2245
                     /locus_tag="PLN75_03890"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRG03292.1"
                     /translation="MKQLIRNARIVNEGHITKGSVLIDGEFIERIFSEHENPEVNGVA
                     ETIDAEGLWLLPGVIDDQVHFREPGLIHKGDIYTESRAAVAGGVTSYMEMPNTNPQTT
                     TIELLEEKYALAATKSLANYSFYMGATNTNILELKKVNPAEVCGIKVFMGSSTGNMLV
                     NDIEALNRIFEESPTLVATHCEDEAIIQHNLNYYKSNYSRNIGVEFHPLIRSAEACYK
                     SSSFAVELARKHNTRLHILHLSTAAEISLFSNEIPLKDKMITAEVCVHHLWFSDADYA
                     AYGNKIKWNPAIKKETDRQALIDAVNSNRIDVVATDHAPHTPEEKEGSCYKAASGGPL
                     VQHSLSAMLQMAKNGYFTPEKVVEKMCHAPADLFRIEKRGYIRPGYYADLVLVNPSAT
                     YLVAPENILYKCGWSPFEGETFDAAVVRTFVNGTTVFNNGVFDENVRGKRITFNV"
     gene            2286..3578
                     /locus_tag="PLN75_03895"
     CDS             2286..3578
                     /locus_tag="PLN75_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRG03293.1"
                     /translation="MEIIILSILILFNGFFSLSEIAIVSSKTTRLEQYRSTGKKGAVT
                     ALRLRSDSEMFLSAVQVGITLISLITGAYGGISLAENVVPFFAKFDILRPYAHGISIV
                     LIMFLITYFSIVLGELVPKTIALGNPEKVAIRVAPLINFFSKMFYPFVKLLSASTKLI
                     TTTFGFEKQSEHMTESELRQMIKTASMEGIIEEGQNDIHEKVFNFSDKRARHLMTHRI
                     EVEWLDLNETEEEIRNEIENCQHSKIVCCHDTLDNLAGVLLIRDYYKAILLKKEFRVE
                     DLITQPVVIHEKTSAPIVLKNLKQSSSHFCVVVDEYGDFQGIVTFHDIMENLVGLIPD
                     EGEIEEPDFFVREDKSVLMNGDAPIETLTQIIRELDVDFELIDYATVAGFIIDQVNEI
                     PRVGDKIDYLDYTLEIVDMDGNRIDKILISKKEEKEEQ"
     gene            3582..4610
                     /locus_tag="PLN75_03900"
     CDS             3582..4610
                     /locus_tag="PLN75_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRG03294.1"
                     /translation="MHNMKITGLHAFAALLIVLMTGCATENKEYTFSKGQVYGTYYSI
                     TYLHPRKKDLHRQIEEKFSEFDNSLSTFNPQSIISRINRNDSSVRTDAYFEAMYHMAQ
                     QVSDASSGAFDITVAPLVNAWGFGFGNHDHTMQPNVDSILPFIGYKKISLRNHTLIKN
                     DKRIMLDASAIAKGQSCDVIGRLLEENGCKNFMVEIGGEVFCRGLNPKGEKWHIGIDK
                     PNDDPANEDAELQTIVALSDVGLATSGNYRQYYFRDGRKYAHTIEPRTGSPVNHNLLS
                     ATVVAPTCMQADAYATAFMVLGVDSSMAICQSNPQMDCYLIYQGEKGELLTAYTPGFA
                     KYFAGEHD"
     gene            4796..5605
                     /gene="bamD"
                     /locus_tag="PLN75_03905"
     CDS             4796..5605
                     /gene="bamD"
                     /locus_tag="PLN75_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRG03295.1"
                     /translation="MKKYILTTLIALLVFTSCSEYQSLLKSTDPELKYDKAVEFFVAE
                     KYDKAITLFDDVTSYFRNTDRSELIINYLAKANMAKKDYLTAGEYYKTYAKSYPAGRF
                     IQEAKFGIAYCYYLDSPDVRLDQAPTVQAVNAFQEYIDLYPESEKVKEANKYLTELND
                     KLAQKELINADLYYNLGTYLGNNYLSAVITAENALKKYPGTKHREDLMIVILESKFQQ
                     ALYSEELLKTDRYQATIDEAYTYKNEYPEGKHIKRTDEILKISEKNIPVVN"
     gene            5675..6007
                     /locus_tag="PLN75_03910"
     CDS             5675..6007
                     /locus_tag="PLN75_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRG03296.1"
                     /translation="MDYKRLNSPLSTITRDMNSLGDKVGNTYEAVAIIGKRANQIEQE
                     LKVELDKKLQEFASYSDSIEEVFENREQIEISRFYEKMPKPTLLATQEFIEDKIYYRN
                     PLKEKLKK"
     gene            6195..7088
                     /locus_tag="PLN75_03915"
     CDS             6195..7088
                     /locus_tag="PLN75_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRG03297.1"
                     /translation="MKIKLFVLALLLSTNWTSAQELKCNLQINSDKIQGSNKSVFTTL
                     QKAATEFMNDRRWTELTYEDNERIECTINIIINTAEGDNFTAELVAQSRRPVFNSSYT
                     TTLLNFRDVNFSFTYREFDPLEFNISSLNSGLTAVLAYYAYLFIGYDMDSFSRLGGTP
                     WFQAAEEIVTQAQSTDWPGWRAFENDRNRYALISNIMDEAFRKYREYFYTYHRLGLDE
                     MVVNATNGRARIAQGIDVLREAYRARPTAVVISSFLDAKNDELINIFKKATPEEKEKV
                     VEILSTVNPSQAARYDQINKK"
     gene            7802..9457
                     /gene="recN"
                     /locus_tag="PLN75_03920"
     CDS             7802..9457
                     /gene="recN"
                     /locus_tag="PLN75_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRG03298.1"
                     /translation="MLKTLHISNYALITELQIDFEAGMSVLTGETGAGKSIIIGALSL
                     IQGLRADTRVVKEGTEKSIVEAGFDVKNYRLKSFFENNDLDYNDSCLIRREIAANGKS
                     RAFINDTPVPLNLLRELSDKLLDIHSQHENLLLSTENYQLMVVDAVAQNKGELSDFQQ
                     AFAAWNEAKAELNKLRKEADRRNADLDYLQFQLHQLDEAKLTEGEQADLETELEMLSH
                     VEEIKSALAQADHLLANEDTNVLKSVKEITTAIRRINTFLPESTSWAERLEAVLIELK
                     DLASDISLAQDKLEFNPERLEWLDNRLNLIYSLQKKFRVETVTELIEMRESFRKQLNR
                     IENLDDELSEAAKRVEQCFRAMQTNSERLTGSRRKAIPVIEKFLVGELTQLGMPAIQF
                     VVSIKTGSEFTEKGNDVVEFLFSANKNRSVLPVSQIASGGEISRLMLGIKSMLISKSD
                     LPTIIFDEIDTGISGEIAGRMGEIMRKMSEGTQVIVITHLPQIAAKGNNHFAVYKDSS
                     GKETETHIRLLPTDERIREIASMLSGKSITEAALMNARELLRN"
     gene            complement(9586..10233)
                     /locus_tag="PLN75_03925"
     CDS             complement(9586..10233)
                     /locus_tag="PLN75_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03299.1"
                     /translation="MNNRLKILIIFILTLIITVGGGYLYYTQLTEIQKMDEEYLTEVH
                     YREPVVIPGVNLGNVNPKKVRRNSKDNEPLIVPDAGRKLPSEGTVGDIETSSTGRLTG
                     FGGHTYPKRTIESNNGGGSGAGGGNMLAYSGSSRSGGSSSAGGYSSGGTMMGGTAPVM
                     APRIPTTSPPTVGTGTILVDPMNDPLESERVPVGDGLWILIALALGYSIKSRKQK"
     gene            complement(10247..13501)
                     /locus_tag="PLN75_03930"
     CDS             complement(10247..13501)
                     /locus_tag="PLN75_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG03300.1"
                     /translation="MKTFYTISIRYLFALLTICLFLFFTVKASAQSQCTDCTSSYNGG
                     NNNTGNTLTGTLCFTEGTNTIQNNISSIADGTIICVNQGATLDLSSNQYNGIQGTVTV
                     NVYGTLKFSTNPALAGNWIFNIFTGGSVSYGSSLEFSGNLTINNQGTFSGGKLAVTGN
                     TSTGTITNTGAMTLTDDLNFAGTTFTFYNNSQTALKLKTIAVSSSSSTLTFRNYTTMN
                     VSSTLNLNNGTAHFLNNGILTIGENYNSSPTSTYVNCGTYTGKFNLNSGGKVINTGTF
                     ITSQIDFGSSASKIENYGTFNSSGSINASGPILNAGIFILSSGQLSGDGNLTGPSAGS
                     ASKGYFVWSGINGKNSGTIGPNLNFRNSSGTSEKTTMFNNSASTQYTWLSNITWGDTE
                     PTTLPPTDCPLPDGKPNVAVPLQSSACAGVDLTTLEPKYSNVTYEWWTGGTSTEKGTQ
                     ITTSTTPSVSNFTTSGITVYLWAKNIFSGVYSNAGAAVNISTCLNFWKGSNDPSTGTD
                     WNTAANWTAGKVPAENEDIVFATAVNNNGSPAINDLVVPAGAAKNIGKLTNDSEKALI
                     IPAGTSVTANGSVSGFDATPEKLRLLAEAGKANGTFIALDNCNTTVLGTVQLYAKGKK
                     GEKITWTDNIEGSPTKDQSFTSSYQWQFFGVPVETVKASPTFDGGFLRKYNEAINKTS
                     GNSSNSVNGSYYYKWETLNNASDLTAFTGYEITQDNPTTYTIKGRLQLCDKTIKLTRS
                     AVEVPGSTDSNEANKRYGLGQNIFGNSFTAAIPVKDLTFPSNVEQTVYLYNTGSFANW
                     TTGSVSETTTDAGSYLSIPYNTSSALDAQIPSMQGFLLLHTGTIGTTIDMTLPYARLT
                     KNLKPQTAPRKALSYLHINLTSNSTRDNLWLFSQQGTTGKLDDGWDGRKFFGTPTAFI
                     YTENADGPMQVNTDQTIDGTVLSFYANTDTEYTLTLAKTNLDEYANLHLIDLQTHTAI
                     PLTKDTTTYRFTARSNGNVIKRFIIANASEINLSDNKFSLLHGYVKDNSRLIISNFTT
                     KKGTVSLYDISGKQITAKEMPVSVSEIPVTAEPGVYILDLQADGKRETIKLIVR"
BASE COUNT         4117 a         3244 c         3179 g         3850 t
ORIGIN      
        1 aacccgaaac ccgaaaccca aaacccgaaa cccaaaactc aaaacccgaa acccgaaacc
       61 cgaaacccga aactcaaaac tccaaactcc aaactaaaaa tatcatgcct tcaaaacttg
      121 tactgatacc gacttacaac gagaaagaaa acattgaaaa tattattcgt gcagtattca
      181 ggctcgcggg tgaatttcac atactagtca ttgatgatgg ttcgcccgat ggtactgccg
      241 gtatcgtaca ccgactgatg agtgaatttc ccaacagtct ttttatactg gaaagggaag
      301 gaaagctcgg actcggcacg gcttacattg ccggtttcag gtgggcgctc gcacgcggat
      361 acgattatat cttcgaaatg gatgccgact tctcccacaa ccccgaagac ctgccccgcc
      421 tttacaatgc ctgtaatgac gaaggtgcgg atgtggcaat cggctccaga tacattagcg
      481 gagtgaatgt tgtcaactgg cccataggcc gggtgctgat gtcgtacttt gcatccaaat
      541 acgtaaggct gataaccgga ctgaaaattg ccgacaccac tgccggtttc aaatgttacc
      601 ggcgccaggt gctggagacc atcgaactgg ataaaatccg gttcaaggga tatgcatttc
      661 agatcgaaat gaaatttaca gcctacaaat gcggatttac cctcaaagaa gtgcccatca
      721 ttttcatcaa cagacgactc ggaacgtcca aaatgagcgg cggaatcttc ggcgaagctt
      781 tcagcggagt tttcaagatg aagataagca gcttctcccg aaaatatcca cagaaaaagg
      841 cctgaacaga ttaatcggtt ttttttatgt cgttttttta cttttaaaaa ctcatccgga
      901 tatgaaacag ttaatcagaa atgcccgtat tgtcaacgaa ggacacatca ccaaaggttc
      961 agtgctgatt gatggtgaat ttatcgagcg gattttcagt gaacatgaaa atccggaagt
     1021 aaatggtgtg gcagaaacga tagacgccga aggtctgtgg ctgctgcccg gagtcattga
     1081 cgaccaggtt cattttcgcg aaccgggatt gattcacaaa ggcgacatct ataccgaaag
     1141 ccgcgctgca gtagccggag gtgtgacttc gtacatggaa atgcccaaca ccaatcctca
     1201 gactaccacc attgaattgc tcgaagagaa atatgccctg gcagccacca aatcgctggc
     1261 caactactcc ttttacatgg gagccaccaa caccaacatc cttgagctga aaaaagtcaa
     1321 tccggcagaa gtctgcggca taaaggtttt catgggttca tccaccggca atatgctggt
     1381 aaacgatatc gaagccctga accgtatttt tgaggagtcg cccacgctgg ttgccacaca
     1441 ttgcgaggac gaagctatca tacagcacaa cctgaactac tacaaaagca attacagcag
     1501 aaacatcgga gtggagtttc acccccttat acgctcggcc gaagcctgct acaaatcttc
     1561 gtcgtttgcc gtagaactgg cccgcaaaca caacacccgc ctgcatatcc tgcatctttc
     1621 caccgcagcc gaaatatcac ttttcagcaa tgaaattccg ctgaaagata aaatgattac
     1681 agccgaagtg tgtgtgcatc atctctggtt ttccgatgcg gattacgctg cctacggcaa
     1741 caaaatcaag tggaatccgg ctataaaaaa ggaaacagac cgccaggcac ttatcgacgc
     1801 agtgaacagc aacaggatag acgtagtagc taccgaccat gctccccata cccccgaaga
     1861 gaaagaggga tcgtgctaca aggcagcttc gggcggaccg ctcgtgcagc attcgctatc
     1921 agccatgctg caaatggcga aaaatggcta ttttacgccc gaaaaggtgg ttgaaaaaat
     1981 gtgtcatgca cctgccgacc tgttcagaat agagaaacgg ggatacattc gcccggggta
     2041 ttatgccgat ctggtgctgg tcaatccttc ggcaacgtat ctggtagctc ctgaaaatat
     2101 actctataaa tgcggctggt cgccttttga aggcgaaaca ttcgatgccg ccgtagtacg
     2161 tacttttgtc aacggcacta cggtattcaa caacggtgtt tttgatgaaa atgtacgcgg
     2221 caaaaggatt acatttaatg tctgatatca atttctgaat tgttaaattc aatttttcac
     2281 actgaatgga aattattatt ctgtcaatac ttattttatt caacggtttt ttttcacttt
     2341 ccgaaatagc cattgtatcg agcaaaacca cccgactgga gcagtacagg agtacaggaa
     2401 aaaaaggagc agtcacagct ttgagacttc gctccgattc cgaaatgttc ctctcagccg
     2461 ttcaagtggg catcacgttg attagcctca ttaccggtgc ttacggagga ataagcctcg
     2521 ctgaaaatgt agtcccgttt tttgcgaaat tcgacatcct ccggccctac gctcacggaa
     2581 taagtattgt gctcatcatg tttctaatca cttatttttc cattgtatta ggcgaacttg
     2641 tgcctaaaac catcgcactc ggcaatccgg agaaagtagc tatccgagtg gcgcccctca
     2701 tcaatttttt cagtaagatg ttttatccct ttgtgaagct actctcggca tccaccaaac
     2761 tgattaccac tacatttggt ttcgaaaaac aatcggaaca catgaccgag tctgaactgc
     2821 ggcagatgat caaaacggca tctatggagg gtatcattga agagggacag aatgatatac
     2881 acgaaaaagt attcaatttt tccgacaaaa gagcccgtca cctcatgaca caccgcatag
     2941 aagtggaatg gctggacctc aacgaaacgg aagaagaaat caggaatgaa atagaaaact
     3001 gccagcacag caagatagtc tgctgccacg atacgcttga taatctggcc ggagtgctcc
     3061 ttatccgcga ttactacaaa gctattttgc tcaaaaaaga attccgtgtg gaagacctca
     3121 ttacacagcc ggtggtgata catgaaaaaa cttcggctcc catagtgctg aaaaatctca
     3181 agcaaagcag ttcgcacttc tgtgtggtgg tggatgaata cggtgatttt cagggaattg
     3241 taacttttca cgatatcatg gaaaatctgg tcggacttat tcccgacgaa ggtgaaatag
     3301 aagaacccga ctttttcgta cgcgaggata aatcggtgct catgaacggc gatgcaccca
     3361 tcgaaaccct tacgcagatt atccgcgaac tcgatgtgga tttcgaactc atcgactacg
     3421 ctaccgtggc aggtttcatt atcgaccagg taaacgaaat accgcgagtg ggcgataaaa
     3481 ttgattacct ggattacaca ctcgaaatag tggatatgga cggtaaccgc attgataaaa
     3541 tactgatttc aaaaaaagaa gaaaaagagg aacaataatc catgcataat atgaaaatta
     3601 caggattaca cgcatttgcc gcgttgctga tagtgcttat gaccggttgt gcaactgaaa
     3661 acaaggaata taccttcagc aaggggcagg tgtacggcac atattactcc atcacttacc
     3721 tgcatccccg gaaaaaggac cttcacaggc agatcgaaga aaaattcagc gaatttgaca
     3781 actcgctttc tactttcaat ccccaatcaa ttatctcacg aatcaaccgc aatgactcat
     3841 ctgtacgcac cgatgcctat tttgaagcaa tgtaccacat ggcgcagcag gtatccgatg
     3901 catcatccgg ggcatttgat atcacggtag caccccttgt caacgcctgg ggatttggat
     3961 ttggtaatca cgatcatacc atgcaaccca atgtggattc aatcctccca ttcattggtt
     4021 acaaaaaaat ttcgctccgc aatcatacac tcatcaaaaa cgataagcgt atcatgctcg
     4081 atgccagtgc aatagccaaa ggtcagagtt gcgatgtgat aggccggctc ctcgaagaaa
     4141 acggatgcaa aaatttcatg gtcgaaatag gtggcgaggt tttctgtcgc ggactcaatc
     4201 cgaaagggga gaagtggcac atcgggattg acaagcccaa cgacgacccc gcgaatgaag
     4261 acgctgaact gcagacaatt gttgcgctta gcgatgtggg acttgctact tcgggcaatt
     4321 accgccagta ctattttcgc gacggaagaa aatatgccca caccatcgaa ccccgcaccg
     4381 gctctcccgt gaatcataat ctgctgagtg ctaccgtagt ggcgccaacc tgcatgcagg
     4441 ccgatgctta cgccaccgct tttatggtac ttggggttga cagcagcatg gccatctgcc
     4501 agtcaaatcc gcaaatggat tgctatctca tttatcaggg tgaaaaaggt gaattgctga
     4561 ccgcctatac tcccggattt gcaaaatact ttgccggtga acatgactga aacgaacggc
     4621 tgctacagtc attattcata aaaaagataa attttttttg aattagatat taaatgacta
     4681 attttgcacc ctgaaattca gtatgaaacg tgtacacgag ggtaaaactt atacagttga
     4741 aacatttttc aacatccggt gtcaatcgct gcaattatat aaaaaatatt tccgaatgaa
     4801 aaaatacatt ctcaccaccc tgatcgccct gctggtattt acttcctgta gcgaatacca
     4861 gagtttactc aagtccaccg atccggaact gaagtacgat aaggctgtgg aattttttgt
     4921 ggctgagaaa tacgataaag ccatcacact cttcgatgat gtgacaagct acttccggaa
     4981 taccgaccgc tctgagctga taatcaatta cctggcaaag gccaatatgg caaagaaaga
     5041 ctatctcaca gccggcgagt attacaaaac ctacgctaaa tcgtatcctg ctggccggtt
     5101 tattcaggaa gccaaattcg gaatagcgta ctgctattac ctcgattcgc ccgatgtgcg
     5161 actcgatcag gcacccaccg tgcaagcagt gaatgcattc caggaataca tagacctcta
     5221 tcccgaatcc gaaaaggtaa aagaggccaa taaatacctc acagaactga atgacaaact
     5281 ggcacaaaaa gaactcatca acgctgatct gtattacaac ctgggtacct acctgggcaa
     5341 taattacctg tcggcagtga taacagcgga gaatgcactg aaaaaatacc ccggaaccaa
     5401 acaccgcgaa gacctgatga tagtcattct cgaatcgaaa tttcagcagg cactttacag
     5461 cgaagaacta ctcaaaaccg accgatatca ggctacaatt gatgaggctt atacctataa
     5521 aaacgaatat ccggaaggaa aacacattaa acgcacagat gaaattctga aaatatcgga
     5581 aaaaaatatc cccgttgtga actaaaaaaa aatgtacaaa aacagataac aaaaatcgat
     5641 aaacaggtaa atcgtatatc gtaaaattaa taatatggat tacaaaagat taaactcacc
     5701 cctttctacc atcacccgtg atatgaattc attgggcgat aaagtgggca acacgtacga
     5761 agcagtggct atcattggta aacgggccaa tcagattgaa caggaactca aagtggaact
     5821 ggacaaaaag ctccaggaat ttgcttccta cagcgacagc atcgaagaag tgtttgaaaa
     5881 ccgtgaacag attgaaatat cacgtttcta cgaaaaaatg ccaaaaccta cactgctggc
     5941 tacacaggaa tttatcgaag ataaaattta ctaccgcaat ccgctgaaag aaaaactgaa
     6001 aaaataagct tatttttcat gttttcattt aatcattact gtttcttgtc catttatttg
     6061 ggcaggaaac ttttattttt taaaaaaaaa tcatgaatac ctgttaatat atttcttcct
     6121 gtaataccaa caatatacga ttttcggaat ttattcgtta tcaacagtgt aaatttttgt
     6181 ttttaatggc agccatgaaa atcaaactct ttgtactcgc actcctactt tcaaccaact
     6241 ggacatcggc tcaggagctg aaatgcaacc tgcagatcaa ttccgataaa attcaggggt
     6301 ccaataagtc tgtcttcacc acgctccaaa aggctgccac cgaatttatg aacgatcggc
     6361 gatggaccga actcacttac gaagacaacg agcggataga atgtaccatc aatatcatca
     6421 tcaatacggc cgaaggcgat aatttcacag ccgaactggt agcacagtcg cgccggccgg
     6481 ttttcaattc gtcctatacc accacgctac tcaatttcag ggatgtaaat ttctctttta
     6541 cgtaccgcga gtttgatccg ctcgagttta acatcagcag cctcaactcc ggactcacag
     6601 ccgttctggc ttactatgca taccttttta tcggttacga tatggattct ttttcgcgcc
     6661 tcggaggcac gccgtggttt caggcagccg aagaaatagt cacacaggcg cagagcacag
     6721 actggcccgg atggcgcgca ttcgaaaacg accgcaaccg ctatgccctt atcagcaaca
     6781 tcatggacga agccttcaga aaataccgcg agtatttcta cacctatcac cggctggggc
     6841 tcgacgaaat ggtggtaaat gccaccaacg gccgtgccag aattgcccaa gggatcgatg
     6901 tgctgcgcga ggcgtaccgc gccagaccta ccgctgtggt tatcagctcc tttctcgatg
     6961 ctaaaaacga tgaactcatc aacattttca agaaagctac acccgaagag aaagaaaaag
     7021 tcgtcgaaat actctcaacc gtcaatccct cacaagctgc ccgctacgac cagataaaca
     7081 agaagtgatt tttcaaacgt ttgaatgttt gtcccgatag ctatcgggag tttggatgtt
     7141 tgattgtttg attttttgat tttttgattt tttgattgat tgattgaatg ttaaacaccc
     7201 gaactcccaa acacccgaac atccgaactt ccgaacttcc gaacttccga acaaccaaac
     7261 attcttaagt cctctgttgg gggatttagg agctaaacat tctaacttta aatctgctct
     7321 gaaaacttta attttcacgc aaatggaaca tcctgagtat cgggagtcgc aaaggcaaaa
     7381 agttttcaac ttgtaaaaca ctgatataca gcatctattg ttagttcgca aagaactcga
     7441 ctcgactttt aaaaacatag agaacaatag tcttatttta ttgaattttc gtgttgtttt
     7501 atttaatttc gataacaata gtttaaaaat atccctgcct gcggccccga caagtcggga
     7561 taaacaaggc agggatattt ttcactatgt ttccgaatat atgagaataa gtataaatta
     7621 ttctatgttt tctattgttt taaaaaagtc aagatgactt caaagattaa aatcttcgat
     7681 tttaatggtt tttgactttt cggagtggac tcaactttct aactttctaa ctttctatct
     7741 gttttttttt ttggaatttt gcctccggaa tcataccttt gcatctaaat cgaactgctc
     7801 aatgctcaaa acgttacata tttccaatta tgcactaatc accgaacttc agattgattt
     7861 tgaagctgga atgtcggtac tgacaggtga aaccggtgcc ggaaaatcga ttatcatcgg
     7921 tgcgctttcg cttattcagg ggctgcgggc agatacacgc gtggtgaaag aaggaaccga
     7981 aaaaagcatc gtagaagccg gatttgatgt gaaaaattac aggctgaaat ctttctttga
     8041 aaataacgac ctcgattaca acgactcatg cctcatacgc cgcgaaatag ccgccaacgg
     8101 gaaatcgcgt gcattcatca acgatacgcc ggtaccctta aatttgctca gggaactgag
     8161 cgacaaactg ctcgatattc attcccagca cgaaaacctg ctgctctcaa ccgaaaacta
     8221 tcagctgatg gtggtagatg ccgtagcgca aaataaagga gaactttcgg attttcagca
     8281 ggcatttgct gcctggaacg aagcaaaagc cgaactgaac aaactccgga aagaagccga
     8341 ccgccgcaat gccgacctgg actatctgca gtttcaactc caccaactgg atgaagcaaa
     8401 actcaccgaa ggcgaacagg cagacctgga aaccgaactc gagatgctga gtcatgtcga
     8461 agagataaaa tcggcactcg cacaggctga tcacctgctt gcaaacgaag acaccaacgt
     8521 gctgaaatcg gtcaaagaaa taaccacagc cattcgccgg attaatacct ttttacccga
     8581 aagtaccagc tgggccgaac ggttggaagc cgttttgatt gaactgaaag acttggcttc
     8641 cgatatttcg ctggcacagg ataaactgga gttcaacccc gaaagactcg aatggctgga
     8701 caaccggttg aacctgattt attctcttca gaaaaaattc agggtggaaa cagtgacaga
     8761 gttgattgaa atgcgtgaat cgttcagaaa gcagttgaac cgcatcgaaa atctggacga
     8821 tgaactcagc gaagcagcga aacgggtgga gcaatgcttc cgggcgatgc aaaccaactc
     8881 cgaacgactc accgggtcgc gccgtaaagc cataccggtc atcgaaaaat tcctggtcgg
     8941 tgagctcacc caactcggaa tgcctgccat acagttcgtg gtaagcatca aaaccggcag
     9001 cgaatttaca gaaaaaggaa acgacgtggt cgaatttctt ttctcggcaa acaaaaaccg
     9061 cagcgtgctt cccgtatcgc aaattgcttc gggtggcgaa atatcgcgcc ttatgctcgg
     9121 aataaaatcc atgcttatca gcaaatctga tctgccaacc attattttcg acgaaataga
     9181 taccggcatc tccggcgaaa ttgccggtag gatgggcgaa atcatgcgaa aaatgagtga
     9241 aggcactcag gttattgtca tcacacatct gcctcagata gccgccaaag gcaacaacca
     9301 tttcgctgtt tacaaagaca gctccggcaa agaaaccgaa acacacatcc gcctgctccc
     9361 aaccgacgaa cgaatccgcg aaatagcctc catgctcagc ggaaaatcca tcaccgaagc
     9421 agccctgatg aatgcccgcg aactgctgag gaattgaaaa cagcccccac catcaattgc
     9481 ctgctgcttt aggcagctaa atttatattg atttccacct tatcagtgag gaggcaattc
     9541 aaccaatacc gggagagaat aaatggagag agttggaact ctgtgttatt tctgttttct
     9601 gctcttaatg gaatacccca atgccagcgc aataagtatc cacagtccat ccccaacggg
     9661 tactctttca gattcgagcg gatcattcat cggatcgacc agaatggtgc ctgtaccgac
     9721 ggtgggaggg gaagtagttg ggattcgggg cgccattaca ggtgctgtgc cgcccatcat
     9781 tgtacctccg gagctgtacc cgcctgcaga gctactgcct ccactgcggc tgcttccact
     9841 gtatgccagc atatttccgc cgccggctcc actaccaccg ccgttgttcg attcgatggt
     9901 tcgtttcggg taggtgtgac cgccgaagcc cgtgagtctg cccgttgaag aagtttctat
     9961 gtctcccaca gttccttccg acgggagttt tctgccggca tcgggcacga taaggggttc
    10021 gttgtctttg gagtttctgc gaactttctt cggatttaca tttccaagat tcactccggg
    10081 aatgactacc ggctcccggt aatgcacctc cgtaaggtat tcttcgtcca ttttctgtat
    10141 ttcggtgagc tgggtgtagt aaaggtagcc tccacctacg gtgataatga gtgtaagaat
    10201 aaaaatgatc agtattttca gccggttgtt catatcctct acgcccttat ctgacgatca
    10261 gtttaatcgt ttcccgtttg ccgtcggctt gcaaatccag tatataaaca ccaggttcgg
    10321 ctgttaccgg tatctcgctc accgaaaccg gcatttcttt agcggtaatc tgtttcccgg
    10381 atatatcata caggcttact gtcccttttt tggtggtgaa gttgctgatt atcaaccggc
    10441 tgttgtcttt tacgtatccg tggagcagac tgaacttgtt gtcgctcaga tttatttctg
    10501 acgcattggc tatgataaac cttttaatca cattgccgtt gcttctggca gtgaagcggt
    10561 aggtagtcgt atccttcgtc agtggaattg ccgtatgtgt ctgaaggtca atcaggtgca
    10621 ggtttgcgta ctcatccagg ttcgtttttg caagtgtgag ggtgtattcc gtgtcggtgt
    10681 tggcatagaa gctcagcacc gttccatcga tggtttggtc ggtattcacc tgcatcggtc
    10741 cgtcggcatt ttcggtgtag ataaatgccg tgggagtacc gaagaatttc cgtccgtccc
    10801 agccatcgtc cagtttgccg gtagtgcctt gctggctgaa gagccagagg ttgtcgcggg
    10861 tggaattact ggtcagattt atatgcagat aagataaagc tttgcgcgga gcagtctggg
    10921 gtttcaggtt tttggtcagc ctggcgtatg gtaatgtcat atcgatagta gtgccgatag
    10981 ttccggtatg gagcagcagg aagccctgca tggaaggaat ctgtgcatcg agtgccgatg
    11041 aagtgttgta aggaatggaa aggtaagaac cggcatcggt agtcgtttct gagacacttc
    11101 cggttgtcca gttggcaaac gaaccggtat tgtacaaata taccgtttgt tcaacattgg
    11161 aaggaaatgt caggtctttg accggaatag cagccgtaaa ggagtttccg aagatatttt
    11221 gccccaggcc atatcgtttg ttggcttcgt tgctgtcagt actgcccggc acttccaccg
    11281 ccgaacgggt gagtttgatt gttttgtcgc aaagctgaag ccggcctttg atcgtgtagg
    11341 tagtcggatt gtcctgggtg atttcatacc cggtgaatgc cgtcaggtcg ctggcattgt
    11401 tgagcgtttc ccatttgtaa tagtaagaac cgtttacgga gttgctgctg ttacctgatg
    11461 ttttattgat tgcttcgttg tattttcgca ggaaaccgcc atcgaacgtg ggcgaagctt
    11521 tcacggtttc caccggcact ccgaagaact gccactgata gctggatgtg aacgattgat
    11581 ctttcgttgg acttccttcg atattatccg tccatgttat tttttcccct tttttacctt
    11641 ttgcatagag ttgaacggtt ccgagtacgg ttgtgttgca gttatccagc gcaatgaatg
    11701 ttccgttagc ctttcctgct tcagccagca gccggagttt ttcgggagta gcatcaaatc
    11761 ccgatacact gccgtttgcc gttaccgatg ttccggcggg aataatcagc gctttttcgg
    11821 aatcattagt cagtttaccg atattttttg ccgctccggc aggtactacc aggtcattga
    11881 tggcaggaga gccgttgtta ttaactgctg ttgcaaacac gatgtcttca ttctcagcag
    11941 gtactttacc ggcagtccag ttggctgctg tgttccagtc ggtgcctgta gatggatcgt
    12001 tagagccttt ccagaagttc agacatgttg aaatgttgac tgcagctcct gcgtttgaat
    12061 aaactcctga gaagatattt ttagcccaca ggtaaacagt aattccggag gtagtgaagt
    12121 tagatacaga aggagtggta gaggtggtaa tctgagttcc tttttctgtt gatgttcctc
    12181 ctgtccacca ttcgtaggtc acattcgaat attttggttc taaagtggtc agatcaactc
    12241 ctgcacaagc tgacgattgc agcggtacag ccacattagg ctttccatcc ggtagcggac
    12301 aatcggtcgg aggcagtgtt gttggctcag tatcgcccca ggtgatattt gataaccagg
    12361 tgtattgagt ggaagcggag ttattaaaca tggttgtttt ttcacttgtt ccgctgctgt
    12421 ttctgaagtt caggttcggt ccgatagttc cactgttttt gccatttata ccggaccata
    12481 cgaaatatcc tttggaagcc gaacccgcag aaggccctgt caggttgcca tcacccgata
    12541 actgacctga gcttaaaata aatatccctg catttaagat tggtccagaa gcatttattg
    12601 aaccggaaga gttgaaagtg ccgtaatttt ctattttcga agccgaactg ccaaaatcaa
    12661 tttgtgatgt gatgaatgta ccggtattta tcactttccc gccgctgttc aggttgaatt
    12721 ttcctgtata tgtaccgcag tttacatacg ttgaggtagg tgaactgttg taattttcac
    12781 cgattgtaag aattccgtta ttcaggaagt gagccgtacc gttattgagg ttgagtgtgc
    12841 tcgagacatt cattgtagta taatttctga atgtcagcgt ggaagaggaa gaactcacag
    12901 ctatggtttt gagtttcaac gctgtttgag aattgttata aaaggtaaag gtagttcccg
    12961 caaaattcag gtcatcggtc agggtcatgg cacctgtatt tgtaattgta cctgttgagg
    13021 tgtttccggt tacagccagt ttacctcctg aaaatgtccc ctgattattt atcgtcagat
    13081 tacctgaaaa ttctaatgac gaaccataac tcacagaacc tcctgtgaat atattaaata
    13141 tccagttacc ggctaaggct gggttggtgc tgaacttcag agtgccgtaa acattcactg
    13201 taaccgttcc ttgtatgccg ttgtattgat ttgaactaag atcgagtgtg gctccctgat
    13261 ttacacagat tatagttccg tcggcaatgg aggatatatt attctggatg gtatttgttc
    13321 cctcggtaaa acaaagtgta ccggttagag tattacctgt attgttgttt ccaccgttgt
    13381 aggaagaagt acaatcggta cattgagatt gagcagaagc tttgactgtg aaaaacagaa
    13441 acagacagat tgtcagaaga gcaaataaat atctgattga aattgtataa aaagttttca
    13501 ttttgaaaaa tacagatagt aataaatagt tttaatacag tgaattcttt ctgttttgaa
    13561 aggttttttg atttgttcag gttgtttcga aatcgttggt tttttctcaa attgcgattc
    13621 agaaaacggt atgctgaccg gaatcatcaa aaaataggtt gcagctttct gcccttacag
    13681 atccaacagc tcggtaaacc aatgcgctgt agtgataaag aatgatttgt agtaaaattt
    13741 cataattttc tataattaag tagcttggta ttcggtttcg ttagtagcgt tgtgtgttcg
    13801 tgcacaagtt gctcacaaag catatagtta tgacagcagg caccattcat tctatcgtag
    13861 caggtgtatt gttgtctgat tctccgcata ttgcgccgga ataaaaaatc gtagattttt
    13921 ttcattcacc gcgagttgag cggcacatga actcacagac acgggcggtt gggccattgt
    13981 ccaatgattg aatgaaaatt ttttgaatga aaaagattaa ggtggaaaac cgtaatgctg
    14041 tcatctgaaa tatcggtgat tggtagataa taaatcagaa tgtaactttt agttcgattt
    14101 tcgttgcaaa tttattatta attgttggat tagacaaatg aatgtaattg actgctcgtg
    14161 tacccattta acgattaccg gaataaatta gtatcacgat atgttaataa tgtacttatt
    14221 ttgagccgtt tcgaaagtca ttgcaaagtt tttgatacta ttttgatacc cttaaatgcc
    14281 gtaaacgttt aaaataaatt agtatagttt aacacaatta gctttcaatt ttcattggtg
    14341 aatacgctta acgtgcgcca acagacgggc aggctgtttt tttattcatc
//
LOCUS       DAORKP010000044        14363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_347_length_14363_cov_9.733646, whole genome shotgun sequence.
ACCESSION   DAORKP010000044 DAORKP010000000
VERSION     DAORKP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14363
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_347_length_14363_cov_9.733646"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..842)
                     /locus_tag="PLN75_03935"
     CDS             complement(90..842)
                     /locus_tag="PLN75_03935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRG03301.1"
                     /translation="MRESNYYIETTESTNSTLKILSDKESLPEGYVVYTDFQTAGKGQ
                     PGNSWESEKGKNLLFSILLFPQNIPIQSQFILSQITCVAIKKVLDEYTDDISIKWPND
                     IYWKEKKICGILIENSLLRDKINKCIIGIGLNINQEVFASNAPNPVSLKQITGKEMDR
                     EKILFDIHSQLFHLYQNPDFENIQKEYHRNLYRKDGFHPYKDAQTKEHFLARIEKVEP
                     DGRLILITEAGERREYYFKEVAPSKSPPKGET"
     gene            complement(839..2308)
                     /gene="dacB"
                     /locus_tag="PLN75_03940"
     CDS             complement(839..2308)
                     /gene="dacB"
                     /locus_tag="PLN75_03940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRG03302.1"
                     /translation="MKIKHISQSNSYDFQKRHECVSIRRVVGVFMLFFNILLVAQNPV
                     NGFFNNNLHRNATISLLVKDLKTDKVLNSHRAGNAATPASTVKLITTATALELLGADY
                     QFVTTLEIDGKITADSILNGNLYISGSGDPTLGSSKLGDTGFLTGWTAAVRKAGIRQI
                     NGDIIANGSAYDTEGVNPKWIWEDLGNYFAAGAYGLSYKDNTFEMELKSGAVGTTPEI
                     VKITPDIPELSFKNYLKSTTIEFDSAYFYGAPYENQRSIYGEIPANRPVFRIKGDIPR
                     PGLILARDFLWKLKSSGISVSGKATDEFLSVNRKVIYTHKSPILSLIIKEINTNSNNH
                     YAEHLFRHLTLKNGKPVTTTAAVKRIRNFWQSKGLPVDELFLYDGSGLSPLNAVSANF
                     FVSLLSYMDKSGNSATFKASLPISGKSGTLKNFLKSTPLEGKVAAKSGSFTMVKSYAG
                     YIQTNGRKIAFAIIVNNPNSNSLTPTTRKIEEFLINVSK"
     gene            complement(2393..3481)
                     /gene="pdxB"
                     /locus_tag="PLN75_03945"
     CDS             complement(2393..3481)
                     /gene="pdxB"
                     /locus_tag="PLN75_03945"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HRG03303.1"
                     /translation="MKIIIDKNIPYIKGALDRKAEVEYLTYKEMTPDAVRDADALIIR
                     TRTKCNHNLLDGSRVKIIATATIGYDHIDTKYCNENGIKWVNAPGCNALSVTQYMASV
                     LIHLAEKNNFDLSSKTLGIVGVGNVGSRVAKLAEGFGMQVLLNDPPRARVEGSHQFVS
                     LKEIAENADIIAFHPFLNFEGIDKTFHLADDDFFSLLARKPIIINASRGEVVETSALK
                     KAIKNGLISDVVLDCWENEPNIDLELLEKVVISTPHIAGYSADGKANATTQSVQSVSR
                     FFNLGSDNWEPNALPDGLKGDFSVLSFTEFFKKTYNILSDSDLLKSSPETFEQQRSNY
                     PVRREPKAYKGNLPEGFEEKFGVFFLNP"
     gene            3623..4420
                     /locus_tag="PLN75_03950"
     CDS             3623..4420
                     /locus_tag="PLN75_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRG03304.1"
                     /translation="MELENFLNVGEYKLFYRLLKYSENQDEPVLIFLHDSWGCVEMWE
                     NFPEKISELFGLNALIYDRRGYGKSSRFSDEIRTKRYLHEASDELIKVMNVMKIEKAI
                     LYGHSDGGTIALIAAATQPDRFQAIIVEGAHSFVEEKGKAAVRESRDKALTNSLLQSL
                     RKYHGEKTAELFRRWHEAWLGDFFADWTIVPILKNIRCPVLAFRGENDPFDTIGQLHV
                     LEKEITAPVIAKVIPDAAHTPRKENEEETMELIGHFLDKGLMRGKIC"
     gene            4543..4923
                     /locus_tag="PLN75_03955"
     CDS             4543..4923
                     /locus_tag="PLN75_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRG03305.1"
                     /translation="MGIFSALMGNAGSIDREKLEKEYSKLLIESEVIELGFKLIRDTF
                     IFTNKRMIMIDVQGVTGNKLEYFSVPYKSISRFSIESAGTFDLEAELKIWISSEQHPS
                     IRKNFTRSVDVYEVQKVLAHYVMK"
     gene            5015..5371
                     /gene="folB"
                     /locus_tag="PLN75_03960"
     CDS             5015..5371
                     /gene="folB"
                     /locus_tag="PLN75_03960"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRG03306.1"
                     /translation="MSTITLQNMKFHAYHGCLDFEKRDGNTFLVNLILELNTEKAEIS
                     DKLEDTLNYQEVYDVVKHEMEIPSNLIEHAARRIFDALIENFPQILHLNLRLSKLNPP
                     LGGDVKSVSVEIRKNY"
     gene            5435..6967
                     /locus_tag="PLN75_03965"
     CDS             5435..6967
                     /locus_tag="PLN75_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRG03307.1"
                     /translation="MSQSITQKHPKGLYFIYTVAIAERFGYYGMRALFVLYMIKALAM
                     DKELSSVIYGNYTGLVYLTPLIGGYVADKYWGMRRSMFWGALLMVVGQLFMFLSALNF
                     QQVELAKWLMYIGLGALIFGNGFFKPNVSTFVGQLYEPGDRRLDSAYTIFYMGVNLGA
                     FISPLVCGYLGDTGNPADFKWGFLAAAVVSVLCLLIYVMQKEKYLIGPDGKPLGVVPA
                     GKKHTLENETKPVVKFTFREIGFWILGAVGLFALLLFTSDYDVIGSFIYAMCIVVPAF
                     VIADKSLSKIERERIGVIYIIAFFVIFFWSAFEQAGISLTYFAEEQTNRNILGWTMPA
                     SWFQSFNAVFVVILAPVFAKLWVKLGQKDMEPASPVKQAIGLFLLSMGYLLIAFGVRG
                     IEPGMKVSVLWLTGLYFIHTMGELCLSPIGLSMVNKLAPVRFASLLMGVWYLSMATAN
                     KFAGALSTLYPEAGKSRMFFGVEISNLFDFFMIFVVISAVAAIILFGLSKWLQKLMHG
                     VK"
     gene            complement(7107..7598)
                     /locus_tag="PLN75_03970"
     CDS             complement(7107..7598)
                     /locus_tag="PLN75_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRG03308.1"
                     /translation="MKKIGLITDTHGLLDERIYKHFEEVDEIWHAGDWGTMDVVNKLR
                     AFKPVRGVFGNVDGQDIRMTFPKHNRFRCEEVEVWLTHIGGYPGKYDAEVKQEIYKNP
                     PALFVCGHSHILRVMYDETLHMLYINSGSAGKYGFHQVQTLIRFEIEGDKIQNLEVIE
                     LNP"
     gene            7868..9493
                     /locus_tag="PLN75_03975"
     CDS             7868..9493
                     /locus_tag="PLN75_03975"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRG03309.1"
                     /translation="MDTTKYIFVTGGVVSSLGKGIIAASLGKLLQSRGFRVTIQKLDP
                     YINIDPGTLNPYEHGECYVTVDGQETDLDLGHYERFLGVETHRENNITTGRIYQEVIN
                     KERRGDYLGKTVQIIPHITDEIKRNIKSFSDKGFDFVITEIGGTVGDIESLPYIESVR
                     QLKWELGKDCLIIHLTYVPFIAAAGELKSKPTQHSVKLLQEQGVQPDILILRTEHDIP
                     TDLRRKIALFCNVEPHAVLQSIDASTIYRVPLNMHREKLDDVVLTKMDFDLKKTPEAD
                     MREWILFVEKLENATQTVKIGLVGKYVQLPDAYMSIIESLKHASAYNDKKLELKLILS
                     DDLTDKNAAQKLTGLDGIVVAPGFGQRGMEGKFAALKYTRERNIPCFGICMGMQTMCI
                     EFARNVLGYTDANSTEIDPATSHKIVDIMEAQKHILNMGGSMRLGAYKCELKKGSKVY
                     EIYNKSEISERHRHRYEFNNAYKDEFEKAGMTCSGRNDTLDLVEIIELTSHKWYIGVQ
                     FHPEYSSTVIHPHPLFVNFVKAAVSPGLPKGEE"
     gene            complement(9569..12865)
                     /gene="carB"
                     /locus_tag="PLN75_03980"
     CDS             complement(9569..12865)
                     /gene="carB"
                     /locus_tag="PLN75_03980"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRG03310.1"
                     /translation="MRGQVRKVLLLGSGALKIGEAGEFDYSGSQALKALKEEGIETVL
                     INPNIATVQTSEGIADKVYFLPVTPDFVEKVIEKERPDSIFLSFGGQTALNCGVALFK
                     NKILEKYNVRVLGTPVQSIIDTEDREIFNQKLAEIGVKYIQSEAVTSTEEALRAANEL
                     GYPVIVRAAYALGGLGSGFCDNDDELRTLATKAFNYSPQVLVEKSLKGWKEIEYEVVR
                     DRYDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNSEYYKLREIAIRIVRHIGIVGE
                     CNVQYAFDPFSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLPELKNSV
                     TKETSAFFEPALDYIVCKIPRWDLAKFHGVSREIGSSMKSVGEIMSIGRSFEEAFQKG
                     LRMIQQGMHGFVGNKELYTSELDNELSTPTDKRVFYLSQAFEQGYTVNRIYDLTKIDR
                     WFLYKLKNIIDTAHLIEKCADKEHLPTALFSKAKKIGFSDYQIEKLIYKKPMPKDTIR
                     NERIKRGIVPVVKQIDTLAAEYPAQTNYLYLTYNGVANDTPTNSPKGGELVRTMHHET
                     QNINNSPLTSPPLEGLGEAVSAIVLGSGAYRIGSSVEFDWCSVNALNTIRKEGLRSVM
                     INYNPETVSTDYDMCDSLYFDELSYERVLDIIELENPKGVIVSTGGQIPNNLAVRLSQ
                     SGVNILGTQAADIDNAEDRHKFSSMLDRLGIDQPRWKELSTLEDIHQFVDEVGFPLLV
                     RPSYVLSGAAMNVCSNRSELERFLTLATEVSKEHPVVVSEFIEGAKEIEIDAVAQNGE
                     LVVYAISEHVEYAGVHSGDATIQFPPQKIYTQTVRRIKKIAREIARELHIFGPFNIQF
                     LAKDNDIKVIECNLRASRSFPFVSKVLKINFIELATQVMLGYEVKKPEKSAFDLDYVG
                     IKASQFSFTRLQKADPVLGVDMASTGEVGCIGTHFDDALLTAMLSVGYRIPEKNIVVS
                     SGSAKSKVDLLDACRLLVLHGYNLFATGGTQKFLEENGVNSTYVGWPDEEGDLKVTDI
                     IANKNVDLVINIPKNLTERELTNGYKIRRGAIDFNIPLITNARLASAFIKAFCKLKKE
                     DIEIKHWGEY"
     gene            complement(13040..14095)
                     /gene="carA"
                     /locus_tag="PLN75_03985"
                     /pseudo
     CDS             complement(13040..14095)
                     /gene="carA"
                     /locus_tag="PLN75_03985"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
BASE COUNT         3897 a         3014 c         3162 g         4290 t
ORIGIN      
        1 cccctaaatc cctcccgaaa caagtacggg acaggctcga aaggggactt ttaaattgca
       61 actgtataaa aaattctata atccactctt taagtctccc ccttcggggg agatttagag
      121 ggggccactt ctttaaaata atactctctt ctttcaccgg cttctgtaat cagaattaat
      181 ctcccgtccg gctccacttt ttcgatacga gccaggaaat gctcttttgt ttgtgcatct
      241 ttgtaggggt gaaatccatc ttttcggtag agattccggt ggtattcttt ctgaatattc
      301 tcaaaatccg ggttttggta tagatggaat agctggctgt ggatatcaaa aagaattttt
      361 tcacgatcca tttcctttcc ggtaatttgc tttaatgaaa cagggttggg agcgttgctt
      421 gcaaatactt cctgattgat gttcagtccg attccgatga tgcacttatt gattttatcc
      481 cgtaacaaag aattttcaat cagaattccg caaatcttct tttctttcca gtaaatatcg
      541 ttaggccact tgatggaaat atcgtccgtg tattcgtcca gcactttttt gatggccaca
      601 caggtaatct gagagagaat aaactgactt tgaatgggaa tattttgcgg aaaaagcaag
      661 atgctgaaga gcagattttt acctttttcc gattcccacg agttaccggg ctgacctttt
      721 ccggctgtct gaaaatcggt gtaaaccaca taaccttcgg gcagcgattc tttgtcggaa
      781 agtattttca gcgtactgtt ggttgattcg gtggtttcga tgtagtaatt tgattctctc
      841 attttgatac atttataaga aattcttcta ttttgcgggt ggttggtgtg agtgagtttg
      901 agtttggatt gtttacaatg atggcaaaag ctatttttct gccgtttgtt tgaatgtatc
      961 cggcgtaact tttaaccatg gtaaacgacc cgcttttggc tgctactttc ccttcgagtg
     1021 gagtactttt cagaaaattt ttcaatgttc ccgattttcc ggatatgggc agtgaagctt
     1081 tgaatgttgc gctgtttccg gatttatcca tataactgag caaacttaca aaaaagttgg
     1141 ccgaaacggc atttaaaggt gataatcccg aaccgtcgta cagaaaaagt tcatccaccg
     1201 gtaatccttt cgactgccag aaattcctga tacgttttac agccgcggtg gtagtgaccg
     1261 gctttccgtt tttcagggtc aggtgtctga ataggtgctc ggcataatga ttgttgctgt
     1321 tggtatttat ttctttgatt atcaagctta aaataggtga tttatgagtg taaatcactt
     1381 ttctgtttac cgaaagaaat tcgtctgtgg cttttccgct gacagagatg ccggacgatt
     1441 tcagtttcca taaaaaatcg cgtgccaaaa tcaatccagg gcgtggaata tcacctttta
     1501 tacgaaaaac cggtctgttt gccggaattt cgccgtagat gctacgttgg ttttcatacg
     1561 gtgctccgta aaaataagca ctgtcgaact cgatggtcgt acttttcagg taatttttga
     1621 aggacagttc ggggatatct ggtgtgattt ttacaatttc aggcgtagta ccaactgctc
     1681 ccgatttcaa ctccatttca aaggtgttgt ctttgtatga aaggccgtaa gcgccggctg
     1741 caaagtaatt tccaagatct tcccatatcc atttcggatt cacaccttcg gtgtcatagg
     1801 ccgaaccatt ggcaatgata tcgccgttta tctgcctgat tccggctttt cgcactgcag
     1861 cagtccaacc ggtcagaaat ccggtgtcac cgagttttga agaacctagc gtggggtcgc
     1921 cactacccga aatgtagagg tttccattca gaatactgtc ggcagtgatt tttccatcaa
     1981 tttccagtgt ggtgacgaac tgataatcag caccaagcag ttccagagct gttgcggtag
     2041 ttatcagttt gacggtagaa gcaggagtgg cggcattccc ggcccgatgg ctgtttagca
     2101 ccttgtcagt tttcagatcc ttaaccaaca ggctgatagt ggcgtttcgg tgaagattgt
     2161 tgttaaaaaa accattaaca ggattttggg caacaaggag aatattgaaa aacagcataa
     2221 acacgcccac tactctccta atggaaacac attcgtgtcg cttttgaaaa tcataactgt
     2281 ttgattgact tatatgttta attttcattg attttcgatt tatttcataa aattaccaat
     2341 ctgaataaaa atacaaacca gttcttcatt ttaagtatta cagtgccttg tattaaggat
     2401 tcaaaaaaaa cactccgaac ttctcctcaa atccttccgg taaattccct ttatacgctt
     2461 tcggttcccg acgaaccggg taattggagc gttgttgctc aaaagtctca ggtgaggatt
     2521 ttagcaaatc actgtcagaa agaatgttat atgttttttt gaaaaattct gtaaaggaaa
     2581 gaacgctgaa atctcctttc aatccgtcag gtaacgcatt tggttcccaa ttatctgatc
     2641 caagattgaa aaaacggctt accgattgca cgctttgtgt agtggcattc gctttgccgt
     2701 cggcagaata accggcaatg tgaggtgtgg aaataaccac tttttccaac aattccaggt
     2761 caatattcgg ctcattttcc cagcaatcca gcaccacatc tgagatcaag ccatttttta
     2821 tcgccttttt caaagcagaa gtttcaacta cttcaccgcg tgatgcgttg ataatgatcg
     2881 gtttgcgagc aagtaaactg aaaaaatcat cgtccgccag gtggaaggtt ttatcaattc
     2941 cctcaaaatt cagaaacgga tgaaacgcta taatatcagc gttttcagca atttctttca
     3001 aacttacaaa ctgatgactg ccttccacgc gtgcacgagg cgggtcgttc agaagtactt
     3061 gcattccaaa gccttcagcc aacttagcta ctcgtgaacc gacgtttccc acgcctacaa
     3121 tcccgagcgt ttttgaactt aaatcgaagt tgtttttttc ggctaaatga atgagtactg
     3181 aagccatgta ctgagtcaca gaaagtgcgt tgcaaccggg tgcatttacc cattttatgc
     3241 cgttttcatt gcaatatttc gtatcaatgt ggtcgtaacc gatggtggct gtagcgatga
     3301 ttttcacccg ggaaccatcc agtaaattgt ggttgcattt agtccgtgta cggattatga
     3361 gtgcatcagc atcacgcact gcatccggcg tcatctcttt gtatgtcagg tattcaactt
     3421 cggctttcct gtccaacgca cccttgatgt aagggatatt tttatcgata attattttta
     3481 tgcccatatc cggtttatct aaagaagaaa ttgatttttc tactgcaaat ttactcgaaa
     3541 aatgcttaac agtaaatttt tttcggaaat atccataatt gagccaattt gtgtattttt
     3601 gcatgcttat ttatttcgaa aaatggaatt ggaaaacttc ctgaatgttg gagaatacaa
     3661 attattctac agattgctga aatactcaga aaatcaggat gaacctgtgc tcatttttct
     3721 gcacgattcg tggggatgcg tagaaatgtg ggagaatttt ccggaaaaaa tatcggaatt
     3781 gtttggtctg aatgccttga tatacgaccg gagaggctat ggaaaatctt cccgtttttc
     3841 ggatgaaatt cggacaaaaa ggtacttaca tgaagcttcg gatgagttaa taaaagtgat
     3901 gaatgtgatg aaaatagaga aagccattct ctacggccac agcgacggcg gaaccattgc
     3961 cctgatagct gcagcaacgc aaccagatcg ttttcaggcc attatagtgg aaggtgcgca
     4021 tagttttgtg gaggaaaagg gaaaagccgc cgtgcgcgaa agccgtgata aagcccttac
     4081 caattcgtta cttcagtcac tccgaaaata tcacggagaa aaaacggcag agcttttccg
     4141 tcgttggcac gaagcgtggc tgggtgattt ttttgcagac tggaccatcg ttcccatact
     4201 gaaaaatatc cggtgtcctg tgctggcatt tcggggtgag aatgatcctt ttgacacaat
     4261 cggacagctg catgtgcttg agaaggaaat aacagctccg gtaattgcaa aggtaattcc
     4321 ggatgcggct catacaccga ggaaggaaaa tgaagaagag acgatggaac ttattggtca
     4381 ctttttagat aaagggttga tgagaggaaa aatatgctaa tgtgccaatg tgctaatgtg
     4441 ctaatgtgcc aatatgccaa tatgctaatg tgccaatttg atgtcaacta gttaactaat
     4501 aaactaatta accaataaac caattaacca attaactata aaatgggaat attttcagca
     4561 cttatgggca atgccggatc cattgacaga gaaaaactgg aaaaagagta cagtaaactg
     4621 ttgattgaaa gcgaagtgat cgaactgggt ttcaaattga tccgcgatac atttatcttt
     4681 accaataaac gtatgataat gattgatgta cagggagtta cgggaaataa actggagtat
     4741 ttctccgtac cttacaagag tatctcccgt ttcagcatcg agtctgcagg gacatttgac
     4801 ctggaggcag aactgaaaat atggatttcg agcgaacagc atccaagtat ccggaaaaac
     4861 ttcacgcgct cggtggatgt gtacgaagtg cagaaagtgc tggcgcatta tgtgatgaag
     4921 taagtgcccc ccaaccccat aaagggggag ttttgggctg gttaatatat aatttctata
     4981 agaaataatt cccctttagg ggttaggggc agatatgtca acaattactt tacaaaacat
     5041 gaaattccac gcctaccacg gttgtctgga ttttgaaaaa agggatggaa atacgttttt
     5101 agtaaatctg atcctggaat tgaatactga gaaagccgaa atttcagaca aactggagga
     5161 tacgctgaat tatcaggaag tgtatgatgt ggtgaaacat gagatggaaa ttccttccaa
     5221 cctgattgaa cacgcagcaa ggcgcatttt cgatgcttta attgaaaatt ttcctcaaat
     5281 attacacctg aatttacgct tgtccaaact caatccgccg ctcggaggtg atgtgaaaag
     5341 tgtgagtgtg gaaattagaa aaaactattg aattttgaaa aatactcact tcccaatcgt
     5401 aaattgtaaa ttattaattc gtaaatagta ccgaatgtca caatctatca ctcaaaaaca
     5461 tccgaaagga ctctatttta tctataccgt tgccattgcc gagcgtttcg gttactacgg
     5521 catgcgggca ttatttgtac tgtatatgat caaggcgctg gctatggaca aagagctttc
     5581 gtcggttatt tacggcaatt atacgggttt ggtatatctc acgccgctta ttggcggata
     5641 tgtggccgat aaatactggg gcatgcgtcg ttcgatgttt tggggagcct tgttgatggt
     5701 ggtcggacag ctttttatgt ttttaagtgc gctcaatttt cagcaggtgg aattagccaa
     5761 atggctgatg tacattggat tgggagcatt aatttttggc aatggttttt tcaaaccgaa
     5821 tgtttccacc tttgtcggac agctgtatga acccggtgac cgcaggctcg attcggctta
     5881 taccattttc tacatgggag tcaatctcgg ggcatttatt tctccgctgg tgtgtggata
     5941 tttaggcgat acgggcaatc cggccgattt taaatgggga tttctggctg ccgctgtggt
     6001 ttcggtgctc tgtctgctta tctatgtgat gcagaaagag aaatacctga tcggcccgga
     6061 cgggaagccg cttggagtag tgcctgcagg aaagaaacac acgctggaga atgaaacaaa
     6121 accggttgtg aaattcactt tcagggaaat cggattctgg attctgggcg ctgtcggatt
     6181 gtttgctttg ttgctgttta cgtctgatta cgatgtgatt ggttcgttca tttatgccat
     6241 gtgtatcgtt gttccggcat ttgttattgc cgataaatcg ctttcgaaga tcgaacgtga
     6301 gcgcatcgga gtaatttaca tcattgcttt tttcgtgatt ttcttctggt cggcgtttga
     6361 gcaggccggt atttcgctta cctattttgc cgaagagcaa accaatcgga acatccttgg
     6421 atggacgatg ccagcgagtt ggtttcagtc gtttaatgcg gtttttgtgg tgattttggc
     6481 tcctgttttt gccaaactct gggtaaaact tggtcagaaa gacatggaac cggcatcgcc
     6541 tgtgaagcaa gcgatagggc ttttcctgct ttcgatgggt tatctgctta tcgcttttgg
     6601 ggtgcgtggc attgaaccgg gtatgaaagt gagcgtactc tggcttaccg gactttattt
     6661 tatccacacc atgggcgaac tttgtctttc gcccatcgga ctttccatgg taaataaact
     6721 cgcaccggtg cgttttgcat cgttactgat gggtgtgtgg tacctctcaa tggctacggc
     6781 caataaattt gccggtgcgc tgagtacatt atatcctgag gcaggcaaat ccaggatgtt
     6841 tttcggagtc gaaatttcaa acctgttcga tttttttatg atttttgtgg taatttctgc
     6901 cgtagcagcg attattctct tcggactatc caaatggttg cagaagctca tgcacggagt
     6961 gaaataagcg gcccctccat cccgatagct atcgggaccc catggggagg cttttttagt
     7021 tgtttttggg gaaaatgcaa gaaaaattca aaaaaatctc agttttaagt cccattcatt
     7081 actgagcgta atcgaagtaa agggatttag gggtttaact caattacttc cagattctgg
     7141 attttatctc cctcaatttc gaaccggata agtgtctgta cctgatgaaa tccgtatttt
     7201 ccggccgaac cggaattgat gtagagcatg tggagagttt cgtcgtacat cacccgcagt
     7261 atgtgtgaat gaccgcatac gaaaagtgcc ggtgggtttt tgtaaatttc ctgttttact
     7321 tctgcatcat atttacccgg gtaaccgccg atgtgggtga gccacacttc tacttcctcg
     7381 catctgaacc tgttgtgttt cgggaaagtc atacggatat cctgcccgtc cacattcccg
     7441 aaaacacccc gtaccggttt gaaagcgcgc agcttattta ccacgtccat tgtaccccag
     7501 tcgccggcat gccaaatctc gtccacttct tcaaaatgtt tgtaaatacg ctcgtcaagc
     7561 agcccgtggg tgtcggtaat taatccgatt tttttcatat ttccggagga tgttttcctg
     7621 taaaatttaa catgtcaaat ttactgtttt ttttcgtgtt tttcaaattt atactgagtc
     7681 gtcattttaa tgcaaaaaaa ccggttagaa actatttgtt gttttcaggt aaaaatattt
     7741 cagaaaatac attcaatata atataaattg taagatttag taataagtaa accaaaaaaa
     7801 taaaaacaaa gaaaataaat cctggcttat tttatatttt tgtagattaa acatagcaac
     7861 gaaaaaaatg gatactacca aatacatttt cgtaacaggt ggagttgttt catctcttgg
     7921 taaaggaatt attgcagctt cgctcggaaa actgcttcaa tctcgcggtt tcagggtaac
     7981 tattcagaaa ctggatccat acatcaacat tgatcccgga acacttaatc cttacgagca
     8041 tggtgaatgt tacgttaccg tcgacgggca ggaaacagat ctggatttgg gtcattacga
     8101 gcgatttctg ggggtggaaa cgcatcgtga aaacaacatc accaccgggc gtatatatca
     8161 ggaggtaatc aacaaggagc gccggggcga ttatcttggt aaaacggttc agattattcc
     8221 gcacattacc gacgaaataa aacgaaacat taagtcattc agtgacaaag gttttgattt
     8281 tgtaatcacc gaaattggcg gaaccgtagg cgatatcgaa agtttgccgt acatcgaaag
     8341 cgttcgccag ctcaaatggg agttgggtaa agactgcctg atcattcatc tcacttatgt
     8401 gcctttcata gcagctgccg gcgaactgaa atcgaagcct acgcaacatt ctgtaaaact
     8461 gctgcaggaa cagggagttc aacccgatat tttgatcctg agaaccgaac acgatattcc
     8521 gacagatttg cgcagaaaaa tagccttgtt ttgcaacgta gagcctcatg ccgttcttca
     8581 atccatagac gcctcaacca tttaccgagt accgctcaat atgcaccggg agaaactgga
     8641 cgatgtagta cttactaaga tggacttcga cctgaaaaaa acgcccgaag ccgacatgcg
     8701 tgagtggatt ttgttcgttg aaaaactcga aaatgcaaca cagacagtaa aaatcgggct
     8761 tgtaggaaaa tacgtacaat tgccggatgc atacatgtca atcattgaat cgttgaagca
     8821 tgcaagcgca tacaacgata aaaaacttga gctgaaactc atcctctctg atgacctgac
     8881 tgataaaaat gcagctcaga aactaaccgg actcgatggg atagttgttg cacccggatt
     8941 tggtcagcgt ggtatggaag gaaaatttgc tgcactgaag tacacccgtg aacgtaatat
     9001 tccatgtttt ggtatttgta tgggcatgca gaccatgtgt attgaatttg cccggaatgt
     9061 gctcggatac accgacgcca acagtaccga aatagatccg gccacttcac acaaaattgt
     9121 agatatcatg gaggctcaga agcatatcct gaatatggga ggaagcatga gactgggagc
     9181 ctacaaatgt gaactgaaaa aagggagcaa ggtatatgaa atatataata aatccgaaat
     9241 cagcgaacgc caccggcaca ggtatgaatt caacaatgct tacaaagatg aatttgaaaa
     9301 agcaggaatg acctgcagcg gacgcaacga tacactggat cttgtggaaa tcattgagtt
     9361 aaccagtcat aagtggtata taggtgtaca gtttcatccc gaatatagca gcacagttat
     9421 acatccgcat ccactatttg tgaattttgt gaaggcagcg gtctcacctg gcctccccaa
     9481 aggggaggaa taatacagcg gtcaaaatag cagtaatttt gtttctttgc atcaaaaacc
     9541 tccctttcgg ggaggtttgg tggggctttt agtattctcc ccaatgctta atttcaatat
     9601 cttctttctt cagtttacag aaagctttaa taaatgcact tgccaaacgg gcattggtaa
     9661 tgagtggaat attgaaatct attgcccccc ggcggatttt atatccgttt gtcaattccc
     9721 gttccgtcag atttttagga atattgatta ccaaatctac atttttgttt gctataatat
     9781 ctgttacctt caaatcgcct tcttcgtcgg gccagcccac atacgtacta tttacaccgt
     9841 tttcttccag aaatttctga gtaccacccg tggcaaacaa attatacccg tgtagtacca
     9901 gcaggcgaca ggcatccagc agatccacct tcgatttggc gcttccggat gaaacaacaa
     9961 tatttttctc cggaatgcga taacccaccg atagcatggc ggtgagcaat gcatcgtcaa
    10021 aatgagttcc gatgcaaccc acttcgccag tagaagccat atccacaccc aacaccggat
    10081 ctgctttctg caggcgggtg aacgagaact gtgaagcctt aatacctaca tagtccagat
    10141 cgaaagcaga tttctccggt ttttttacct cataaccgag cattacctgt gtagccagct
    10201 caatgaaatt gattttcaaa accttggaaa caaacgggaa tgaacgggaa gccctcaggt
    10261 tgcattctat taccttaata tcgttgtctt tagctaaaaa ctggatatta aagggtccga
    10321 atatatgaag ttctctggct atttcgcggg caattttctt gatacgccgt actgtctgtg
    10381 tgtagatttt ctgcggagga aactgtatag tggcatctcc ggaatgaact ccggcgtatt
    10441 ccacatgttc ggaaatagcg taaaccacca attctccgtt ttgtgcaaca gcgtcaattt
    10501 caatctcctt ggctccttcg ataaattcag aaaccaccac cggatgctct tttgacactt
    10561 ctgtagccag tgtgagaaat cgttcaagct cgcttctgtt cgaacaaaca ttcattgctg
    10621 cacccgaaag cacatacgaa ggacgcacca gtaacggaaa tccgacttca tccacaaact
    10681 gatgaatatc ttccagcgtg ctcagttctt tccacctggg ttggtcgatg cccagccgat
    10741 caagcatcga cgaaaattta tggcgatctt cagcattatc gatgtcagca gcctgagttc
    10801 cgagaatgtt tactccggat tggctcaatc gtactgccag attattagga atttgtccgc
    10861 cggttgaaac aatcactcct ttcggatttt ccagttctat aatgtccagt acacgttcgt
    10921 aacttagttc atcgaaatac aggctgtcgc acatatcgta atcggtcgac accgtttcgg
    10981 gattgtaatt aatcatcacc gaccgcaaac cttccttgcg aatggtattc agagcgttca
    11041 ccgaacacca gtcgaattcc accgacgaac cgatgcggta agcacccgag ccaagcacaa
    11101 tggctgaaac agcctcccca agcccctcca aaggagggga tgtaagaggt gagttgttga
    11161 tgttttgagt ttcatgatgc atcgtccgca ctaattctcc cccttttggg gaattagtgg
    11221 gggtgtcatt ggctactcca ttgtaagtca ggtacaaata attggtctga gccggatatt
    11281 cagctgcgag tgtatcaatc tgcttaacca caggcacaat tccccgtttg atgcgttcgt
    11341 tgcgtatggt gtctttcggc attggttttt tgtaaatcag tttttcaatc tgataatccg
    11401 aaaatccgat tttttttgct ttggagaaca gtgctgtggg cagatgttct ttgtccgcac
    11461 atttctcaat caggtgcgct gtgtcaatga tgtttttaag tttgtataga aaccagcggt
    11521 caatttttgt cagatcgtaa atcctgttca ctgtatagcc ctgctcgaat gcctgcgaaa
    11581 gatagaaaac acgtttatcg gtaggtgtac tcagttcatt gtcgagttcg ctggtgtaca
    11641 attctttatt tccgacaaat ccgtgcatgc cctgctgtat catccgaagt cctttttgaa
    11701 aagcttcttc aaaacttcgt ccgatagaca tgatttcgcc caccgatttc atcgaagaac
    11761 ctatttcgcg gcttacccca tgaaattttg ccagatccca gcgcggtatt ttgcatacga
    11821 tgtaatcaag tgccggttcg aaaaacgcgg aagtttcttt tgttaccgaa ttttttaatt
    11881 cgggaagtcc gtaacccagt ccaagtttgg ctgccacaaa agccagcgga tatccggtgg
    11941 ctttggatgc cagcgctgat gagcggctaa gccgggcatt tacttcgata acgcggtagt
    12001 cttcagaaaa cggatcgaag gcatattgta cattacactc gcctacaatg ccaatgtgtc
    12061 tgacaatacg tatagctatt tcacggagtt tgtagtattc gctgttggaa agtgtctgtg
    12121 atggtgctac tactatactt tcgccggtat gaatgcccag cggatcgaag ttttccatgt
    12181 tacaaaccgt gatacagttg tcgtaacggt cgcgtaccac ttcgtattca atttctttcc
    12241 agcctttcag cgatttttcg accagaacct gtggtgaata attgaaagcc ttcgtggcaa
    12301 gcgttctcag ttcgtcatcg ttgtcgcaaa agccgcttcc gaggccgccc agggcatagg
    12361 ctgcccggac tattaccgga taaccgagtt cgttggctgc acgtaaagcc tcctccgtag
    12421 aggtaaccgc ttcactttga atgtatttta ctcctatttc agcgagcttt tgattgaaaa
    12481 tctcgcggtc ttcggtatca ataatggatt gtaccggtgt accgagtacg cgtacgttgt
    12541 atttctcaag tattttattt ttgaataatg ccacaccgca attcagagcc gtctgaccgc
    12601 caaacgacag aaaaatgctg tcaggacgtt ctttttcaat cactttttca acaaaatcgg
    12661 gtgttaccgg taaaaaataa accttatcgg caatgccttc ggaagtctgc acggtagcga
    12721 tgttcggatt gatcaatacg gtttcaatgc cttcttcttt cagtgctttc agtgcctgcg
    12781 atccggaata gtcaaattcg ccggcttcgc caattttgag agctccggag ccaagcagaa
    12841 gtactttacg cacctggcct ctcactgatt ctccccaaag gggagagaac ccgggcgaaa
    12901 tagagtttgt tgcgttttcg ggagtgtcac ttttttttcc ttttgaagaa aaatcagatg
    12961 aggttaaata ttcttgaatg acagtgttaa cattaatcgc agtacttttt cctcctcctt
    13021 tggaggaggt taggaggagg ctaaacacat caaaaagaaa ctccgtatcc gttggtccgg
    13081 cagatgcttc gggatgaaac tgacaggaga accacggttt ggttttgtga cgaatacctt
    13141 cgttggtgcc gtcgttcaga tttacaaaaa gcggttccca gtcagcgctc aacgtgttgt
    13201 tgtccaccgc aaaaccatga ttttgtgatg taacgaatgc ccgctgtgaa cctatcagtt
    13261 gtaccggctg attgcagctg cggtgtccgt acttcagttt gtaagttttg gctcccccgg
    13321 ccagtgaaag caattggtta cccatacata tgccgaagat gggtttgttg gtttccattg
    13381 atttgcggat attgtccaca gtgatatcac agtatgccgg atcgcccggc ccgtttgaaa
    13441 taaacaatcc atcgtattca attgtattga aatcgtaatc ccagggtact cttatcactg
    13501 ttgtatcacg tttcagcaga cagcggatga tgttgttttt caccccgcaa tccaccagaa
    13561 caactttatt attgccattt ccataggtga ttacttcttt gcagcttgcc tttgccacct
    13621 gattctcgtc atcgggattg acaaacggga tgtccggctg atccgggaaa accagttttc
    13681 cgagcattgt tcctttttct ctgaccagtt tggtcagttc acgtgtgtcg atgccgtaaa
    13741 cggccggaac cttgttttct ttcagccagt cactcaggct ttttacagca ttccaatggc
    13801 tgtattcaaa tgaaaagtcg gaaacgatca gaccgcttgc ctgtattttt tccgactcaa
    13861 aaaaagcaga aataccgttt tgaacttcct ctttcggaac tccgtaattc ccgatgagtg
    13921 gataagtaag agttaaaatc tgaccaaggt aagaagggtc ggaaaggctt tcggtatagc
    13981 cgaccattcc tgtgctgaaa accacttcgc cggctgcagg tgcttcgtaa ccaaaagatt
    14041 tcccttcgaa tactgtaccg tcttcgagaa ttaattgtaa tggcttgttg tacatatggg
    14101 tgtgataatg aaaatgatat gtctgaaaga aatgatttgc acatcaaaaa agttttgcaa
    14161 aattacacat tttctttgaa ttgaggtgca tttatattca tttttcaact taaaaagtat
    14221 aaaatggagc tgttttattc attccggcga aatgaatacc tggatgttaa ataacacaat
    14281 gaaggttcag atcagttttt tttatcagat aataatcacc attttatact gcctggtgtt
    14341 gtttttttta agatagtagt tgt
//
LOCUS       DAORKP010000045        14018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_369_length_14018_cov_8.301010, whole genome shotgun sequence.
ACCESSION   DAORKP010000045 DAORKP010000000
VERSION     DAORKP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_14018_cov_8.301010"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="PLN75_03990"
     CDS             <1..2030
                     /locus_tag="PLN75_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRG03311.1"
                     /translation="YSNKGFQGPALQRRTYEYMWRWGSFFGPYGTYQGTDFRNDIAYR
                     KQAGDWIDSDDFIRLGAFLKADITKGLTLNADYTYNAEFYNYKAAYIPLNGWNSWGGK
                     IDNKVQYVTTSRLEQNSSQNKSFALNIYGNYKFDLLENHHFNLMLGGNAESGNYFSHY
                     SQRDNLLDPKMPEFSLAMGTTQIVSGSHSHWATAGYFGRLNYDYKNIWLLELNGRYDG
                     SSSFPANSRWAFFPSASLGYRISEQEFFSPLKTYISNAKFRASFGEIGNQAVGDNQYL
                     PVLTSNTVNWLLANGDRAVAYNMPKLVASTLKWERIQTLDFGGDFGFFNNDLNVSFDW
                     YQRTTLDMLAPGKTMPDVLGASAPLINAGTLRTRGWELNLDLRHRFNDLSVYANASVG
                     DFKTTIVKWDNDTKLLNTNYSGKTYGDIWGFETERLFSKDDFNTDGTYKTGVASQTAL
                     DQKPFIFGPGDIKFKDLDGSGVIDGGKGTADDHGDLKVIGNFTPRYQYSFRLGGDWKG
                     FDLDMFFQGVGKRSVWTQSAFVMPMMRGADAIYANQTDYWTEENPNPDAKFPRMFPGN
                     AARGTIAVLDLGNHNFYPQSKYLVNMSYLRFKNLTVGYTIPKDLSRKIMMEKARIYFS
                     ANNIAELINNSQAPLDPEININESGASLGNGTWGRIDPMYRTVSFGVQLTF"
     gene            2042..3793
                     /locus_tag="PLN75_03995"
     CDS             2042..3793
                     /locus_tag="PLN75_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03312.1"
                     /translation="MKNNKIMKRIILYICLLSAMTFTACDNFLDREPLDTFTNGNFWT
                     SENNVTGYANSFYGQFTGYGNAGGTGDFYFRTLSDDQAGNSFTDWTFTNVPASSTLWR
                     DGWIEIRRANILLENVDKVPMTDAAKAHWKGFARLMRAWQYYKLIRSYGDLPWVDKSL
                     DITDQGYLYKARESRDMIMDKALEDLNYACENMYDNTSKTVLNKNIAYAMKSEICLYE
                     GTFRKYRKAEDGQTAPDLTGATKFLTEAKTASAYLIDDKRGFKLNASYQGNYNSLDLS
                     KNTEMIMYKVYKQSVLQHSLIAYISSSTQLSGLTKNAFNSYLFTDGLPLALTTKDKND
                     AAEIVYGKKTVAGKTVPDTILSISKLLTLRDKRLVQTIDTAVFYVGRGFTRFGAGISM
                     TSSTGYGISKYDNKDIAAAYRNQTNANFTHAPLFWLAVIYLNYAEAAAELGAITQSDL
                     DMSINKLKTRAGLPALTVAVGFNDPANNMGVSSLIWEIRRERRCELIMDNNNRYWDLV
                     RWHQLDKLDTSAFPDVILGANIANAPAYDKAVPKVGNYIDGSKKLSRKYDKKYYWYPI
                     PSDQISLNDKLTQNPNW"
     gene            4042..5208
                     /locus_tag="PLN75_04000"
     CDS             4042..5208
                     /locus_tag="PLN75_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRG03313.1"
                     /translation="MKNVYFILILLTGSYIVSAQVPPVILGAEQTKAYLPILKNKRVA
                     LFSNHTGMVGNKHTLDILIENNVNVVAIFSPEHGFRGTADAGEHVSSSVDEKTGVPIL
                     SLYDGNLGRPSEENIRKFDVLVVDIQDVGLRFYTYYASMVRLMDACAEFNRKMLILDR
                     PNPNGFYVDGPVLDMKYKSGVGWLPIPVVHGMTLGELALMTNGERWLPASRVCDVTVI
                     PCKNYTHQTRYVLPVPPSPNLPDMKSIYLYPSTCYFEATPVSLGRGTDKPFQVYGHPN
                     MKGYDFTFTPRSISGAKNPPQLDRLCYGVDLSGKSDEEIWEKGIDLSYLIDAYRNLNL
                     DDHFFRPFFELLIGNENIRKMIKSGKSADEIKASWQEDVARFREERKPYLVYSE"
     gene            5294..5674
                     /locus_tag="PLN75_04005"
     CDS             5294..5674
                     /locus_tag="PLN75_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRG03314.1"
                     /translation="MKYRDFKDLIAYQKGFTQAMNIFQLSAGFPVEERYSLTDQIRRS
                     SRSICTNMAEAYRKRAYPKHFHSKMTDCWAENGETGVWLDFAYHCNYISEEIYNQHIA
                     LNTEIGKLITYMINNPEKFGVKED"
     gene            5752..7227
                     /locus_tag="PLN75_04010"
     CDS             5752..7227
                     /locus_tag="PLN75_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRG03315.1"
                     /translation="MSPLAVIITIAAYFLVLFTVSHLAGRKADNAGFFIGNRSSKWYM
                     VAFAMIGSTISGVTFMSVPGMVDPKAFSYMQMVLGFFVGQLIISFVLIPVFYRMKLFS
                     IYEYLDERFGVSTYKTGAWFFFISKVLGASVRLFLVCLFLQMMVFGPLNIPFIFNVVA
                     TVALVWLYTRQGGVKTLIWTDSLKTFCLILSVVLTIFFIVKNMGLDFSGMVSSIRGSD
                     LSRTFFFDNPKEGTYFWKQFLAGIFTMIATTGLDQDFMQRNLSCENPRDSQKNIITSG
                     LVQLVINLLFLMLGVLLYLFIDSKGITRPEKSDELFPMVATQGYLPLAVGILFIIGLI
                     SSAYAAAGSALTALTTSFTVDILGIKNKSEVQIKSTRKNVHIGMAVVMGVVIFVFNLL
                     NNTSVIDAVYILASYTYGPILGMFAFGILIKRSVRDKFVPYIAVLAPIICFFLQKLTI
                     AVWDFHFGYALIIVNALITMAGMFSLSLNPFPKERDWQSKK"
     gene            7266..>9226
                     /locus_tag="PLN75_04015"
     CDS             7266..>9226
                     /locus_tag="PLN75_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRG03316.1"
                     /translation="MKNTGKIHKSLRKIPLGGNNVRRKPIRGLLFVFFFFPFILSAQL
                     LTPDATQNIENYLNSEVSKEISVGKIRIDSTKIAGNELHLFINGNFSNFPFRNHNVAK
                     FNLEIFKLLNLELTKYSLKIFAGTKPVEELILPEFTTKRDKNRKTFSTHTEIPIITRL
                     SAPYKPVNGLQNRHIALWQSHGYYYEQKLARWEWQRARIFQTVEDLYTQSYVLPYLVP
                     MLENAGANVLMPRERDTQTEEIIVDNDEGIHDTSIYSEKNGTKSWSTGNAPGFAQLRK
                     QYVDFENPFKDGTFRQTETSKKGTKTTAEWTPDIPKAGKYAVYISYHTLPKSTEDAYY
                     TVFHRGGKSEFSINQKMGGGTWIYLGTFSFEKGKSGKVVLSNISSKAGQILTADGVKF
                     GGGMGNIARKVSADGATENVPSSRSNEPQAKIENPKIDYPYETSGYPRYTEAARYWMQ
                     WAGIPDSIYSESKGRNDYTDDYKCRGLWVNYLAGGSAVNPLQKGLNIPVDMAFAFHTD
                     AGTTQNDSIIGTLGIYFTGTETGKYPNGVSRNVARELTDRVNSFIVRDIRQLYEPDWT
                     RRGMWDRSYFEARSPNVPTMLLELLSHQNFADMRYGLDPRFRFTVSRAIYKGILQFIA
                     SQNYMNYVVQPLPVDNMSLRFTGGNEVEL"
     assembly_gap    9227..9326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9327..10145
                     /locus_tag="PLN75_04020"
     CDS             <9327..10145
                     /locus_tag="PLN75_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRG03317.1"
                     /translation="ELDHGVPYLNDFKYIGKQKEFRRKIPWMDDDASGFGDSYANYET
                     TVIAGNTFDYPAVHGEAILKAGYSFVSCSDEAVENQTVRLSDYKIIDLILGKEYQTRM
                     GPGGINQLQFKTFNTKIQQAISEYCQQGGNIFISGAFVGTDLWDNRYTVSIEEDKQFA
                     INILKYKWRAGQATRIGNVKAVTSYFPMFVGIYNFYNELNPDNYVVEAPDAIEPGSEG
                     AYTIFRYSENNLSAGVAYKGNYKSCVLGFPFESLKIQIEREKMMKGVLEFFEAP"
     gene            10310..12157
                     /locus_tag="PLN75_04025"
     CDS             10310..12157
                     /locus_tag="PLN75_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRG03318.1"
                     /translation="MKLKLTILFVISQIGVLYGVEPFHFALLTDLHITQNTTAVEDLE
                     NSLKQINNTPEIEFVLVTGDVTEFGDKVSLEKAKELLDKLNIPYYITSGNHETKWSAS
                     GHTDFARIFGDDKFAFEHKGVRFFGFNTGPVLRMADGHVSPQDITWLKDELSKKEKTE
                     PVILATHYPLQYGDVDNWYELTDAVRTHNIRAILGGHYHRNAVFAYDGIPGLINRSNL
                     RAKADVGGYSVYSVTPDSLIAFEQIIGGEPKRWVALSMKEQYFDEKGSDRKYPDFSVN
                     QFFPNVKEKWLMKTGTGIYSSPAIWKKNVFVGDDLGKLTSYNLKNGKKQWSFQTGQRI
                     VGTPAVADGIVVFGSADKNIYGVNAKTGKQIWKIPTNEAVLGTVTIENGIAYIGGSDN
                     SFRAIEVKSGKVNWIFDKLKGYVETKPLIINNKVIFGAWDTFLYALEKNTGNLVWQWT
                     NGIKSTHYSPAAVWPVSANGKVFVVDPERAMTAIDLETGKTSWRTKQSMVRESIGISE
                     DGNRIYAKTMQDSVVCYSSQSETPQKIWSGNIGYGYEHNPSMLIEKSEIVYGSTKNGL
                     IFALEALTGKVIWKHKIGNSLVNTVLPTGKKTVLVTTASGEVSLLKYTP"
     gene            12324..13823
                     /locus_tag="PLN75_04030"
     CDS             12324..13823
                     /locus_tag="PLN75_04030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03319.1"
                     /translation="MTKFSNSPSGIGGRSINCFIPYEEAAQVTQTIQNLKKSTLVDKI
                     YLVATTQLPAVEGCEVIETKSLKSTDAIQKIAAKADADYTMIYTKTTTLKWVNYSIER
                     MVAIAEDSNAAMTYSDHFKEANNVRQEAPVIGYQMGSLRDDFDFGSVLLYRTSALKEA
                     VQRMDADYDFAGLYDLRLKVSQKGDLVHINEFLYYEIENDTRKTGEKIFDYVDPKNRI
                     VQIEMEQACTQHLKDIGGYLEPNFKHIEFTEDNFEMEASVIIPCKNRVKTIGDAIQSA
                     LNQKTTFPYNVIVVDDNSEDGTVDVIKKFMDNPKLIYIAQDTSYHAIGGNWNAAFHDP
                     RCGKFALQLDSDDLYSDENTVQKFVDAFYEQNCAMVVGTYKLTNFDLEMIPPGVIDHK
                     EWTPDNGRNNALRINGLGAPRGFYVPMLRQINFPTTKYGEDYAVGLRVSREYQIGRIY
                     DVIYLCRRWDDNSDASLDVVKMNGHNTYKDRIRTWELQARVKMNKTPNP"
BASE COUNT         4351 a         2809 c         3029 g         3729 t
ORIGIN      
        1 attacagcaa caaaggattc cagggcccgg cattacaacg cagaacatat gaatacatgt
       61 ggagatgggg aagtttcttc ggtccatatg gaacttacca aggcactgac ttccgaaatg
      121 atatagctta ccgtaaacaa gcaggtgatt ggattgacag tgatgatttt attcgtttgg
      181 gagcatttct gaaagctgac atcacaaaag gactaacttt aaatgcagat tacacttata
      241 acgccgaatt ctacaattac aaagcagcct atattccact taacggatgg aattcatggg
      301 gtggaaagat tgataataag gttcaatatg ttacaacatc gcgattagaa caaaattcat
      361 ctcagaacaa atcctttgct cttaacatct acggcaatta caaatttgac cttctggaaa
      421 atcatcattt caacttaatg ctcggaggaa acgccgaaag tggaaattat ttctcccatt
      481 attctcaacg agataatttg ttagacccta aaatgcctga attctcatta gctatgggaa
      541 ccacccaaat tgtaagtggt agccattcac attgggctac tgcaggttat tttggccgat
      601 tgaattatga ttataaaaat atttggttgc ttgaattaaa tggaaggtat gacggttctt
      661 caagtttccc agcaaatagc cgttgggcat ttttcccttc agcctccctg ggttatcgaa
      721 ttagtgagca agaattcttc agcccactaa aaacatacat cagcaatgcc aaatttcgcg
      781 catcttttgg tgaaattggc aatcaggctg ttggagacaa tcaatatctt cccgtattaa
      841 cgagcaatac agtaaactgg cttttggcaa acggagaccg agcagtagct tacaatatgc
      901 caaaattagt tgcatctaca ttgaaatggg aaagaattca aactctggat tttggaggtg
      961 actttggatt tttcaacaat gatttgaatg tttcttttga ctggtatcaa cgaacaacat
     1021 tagatatgct tgctccaggc aagacaatgc ctgatgtact tggtgcttca gctccgttga
     1081 ttaatgcagg tacattacga acccggggtt gggaattgaa tcttgatctg cgtcatcgtt
     1141 tcaatgacct gtcagtttac gccaatgcaa gtgtgggcga tttcaaaaca acaatagtaa
     1201 aatgggataa tgataccaaa ctactgaata caaattatag cggaaaaaca tacggagata
     1261 tctggggatt tgaaacggaa agattattct caaaagatga ttttaatacc gatggaacat
     1321 ataagacagg agtggcttct caaactgcac tcgatcagaa accattcata tttggccccg
     1381 gcgacattaa attcaaagat ttagacggga gcggtgtaat tgatggtgga aaaggtactg
     1441 ctgatgatca tggtgacctc aaagtaatcg gaaactttac accccgctat cagtacagct
     1501 tccgcctggg tggtgactgg aaaggttttg acctggatat gttctttcag ggtgttggta
     1561 aacgctcagt atggacgcaa agcgcattcg taatgcctat gatgcgtggc gctgatgcaa
     1621 tttatgccaa tcaaactgat tactggactg aagaaaaccc aaatccggat gcaaaatttc
     1681 ctagaatgtt cccgggaaat gccgcaaggg gaacaattgc cgtgttggat ttaggaaatc
     1741 acaatttcta tccacagtca aaatatttgg ttaatatgtc atatctccgt ttcaaaaacc
     1801 tgacagtagg ttatacaata ccaaaagatc tgtcacggaa aattatgatg gaaaaggcac
     1861 gtatatattt tagtgccaac aatattgccg aattaatcaa taacagtcag gctccactcg
     1921 atccggaaat caacatcaat gagtcgggag caagtttagg taacggaacc tggggacgaa
     1981 tcgacccaat gtatcgaaca gtgtcatttg gcgttcaact cacattctaa aatcattaac
     2041 gatgaaaaat aataaaatta tgaaacgtat aatattatat atatgcttat tgtcagctat
     2101 gacttttaca gcttgcgaca attttttgga tagagaaccc ctggacactt tcacaaatgg
     2161 caatttttgg acaagtgaaa ataatgttac aggatatgca aactcatttt acggtcagtt
     2221 tacaggatat gggaatgcgg gtggaaccgg cgatttttat ttcagaaccc tctctgacga
     2281 ccaggctgga aattcattta ctgattggac attcactaac gtacctgcca gtagtacact
     2341 ctggagagat ggatggattg aaattcgaag agctaacatt ttattggaaa atgttgataa
     2401 agtaccaatg acagatgcag ccaaagcaca ttggaaaggc tttgcccgat taatgcgtgc
     2461 atggcagtac tacaaactga tacgaagtta tggtgatttg ccctgggttg acaaatcgct
     2521 ggatataacc gaccaaggat atttatataa agcacgagaa agcagagata tgattatgga
     2581 taaagctctt gaagatttga attatgcttg cgaaaacatg tatgataaca cttctaaaac
     2641 tgtcttgaat aagaatatag cctatgccat gaagtcagaa atttgccttt atgaaggaac
     2701 tttcagaaaa tacagaaaag cagaagacgg tcagactgct ccggatttaa cgggtgctac
     2761 taaatttctc acagaagcaa aaacagcatc ggcttatttg attgatgata aaagaggttt
     2821 caagttgaat gcctcctatc agggaaatta caattcactg gacctgagca aaaacactga
     2881 aatgattatg tataaggttt acaaacaaag tgttcttcag cattctttga ttgcttatat
     2941 aagttcctca actcagctaa gcggtttgac aaaaaatgct tttaattcct atttattcac
     3001 cgatggatta cctttggctc ttacaactaa agacaagaac gacgctgctg aaattgttta
     3061 cgggaaaaaa accgttgctg gaaaaaccgt acccgatacc attttatcca tttcaaaatt
     3121 gcttactttg cgagacaaac gtttggttca aacgatcgat actgcagttt tctatgttgg
     3181 acgcgggttt acccgctttg gagctggtat atctatgaca tcatctaccg gttatggaat
     3241 ttcaaaatat gacaataagg acattgcagc tgcatacaga aatcaaacaa atgcaaactt
     3301 cacgcatgct cctctattct ggctggcagt aatctatttg aattatgctg aagctgctgc
     3361 tgaacttgga gctataactc aatctgatct cgacatgtca atcaataaat tgaaaaccag
     3421 agccggcctt ccggcgttga cagttgctgt tggatttaat gaccccgcaa acaacatggg
     3481 tgttagtagt ttaatttggg aaattcgtcg tgaacggcgt tgcgaattaa ttatggacaa
     3541 caacaaccgt tactgggatc tcgtgcggtg gcatcaactt gacaaattgg acacttcagc
     3601 atttccggac gtgattttag gtgcaaatat tgcgaatgct cctgcgtatg acaaagctgt
     3661 accaaaagtt ggcaattaca tagatggatc taaaaagtta tcacgtaaat acgataagaa
     3721 atactattgg tatccaattc catcggatca gatttcatta aatgataaat taactcaaaa
     3781 tcctaattgg taatttaaca ggacaactta taaaggggac atcgattgga tgtcctcttt
     3841 ttaatttaat caatttgaaa atgtcatttt ggagataata ctgaaaaatg attatcaaat
     3901 tgaataattc caacagaatt ttaagaaaat agtaaaccgc cttaaacaca ccggatacaa
     3961 cttgttaaat aattcaaaat aagccaatta aaatctattt ctgaattcaa aataaaagaa
     4021 atttagcgta tttttgttag aatgaaaaat gtgtacttca tactcatcct cctgacgggc
     4081 agttatatcg tttccgccca agttcccccc gtcattctcg gtgccgagca aaccaaagct
     4141 tatctcccca ttctgaaaaa caaaagggtc gcgcttttct cgaaccatac cggcatggtg
     4201 ggcaataaac acacgctgga tattttgatt gagaataacg tgaatgtggt ggcgattttc
     4261 tcaccggagc acggtttccg aggtacggcc gatgccggcg aacacgtttc cagctcggtg
     4321 gatgaaaaaa ccggtgtgcc tatcctttct ttgtacgacg gaaatctcgg tcgtccaagt
     4381 gaggaaaata tccgtaaatt cgatgtgctc gtggtggata ttcaggatgt ggggctgcgt
     4441 ttttacacct attacgcctc tatggtgcgg ctgatggatg cctgtgccga atttaaccgc
     4501 aaaatgctta tcctcgaccg gccgaatccg aacggatttt acgtggacgg gcctgttctg
     4561 gacatgaaat acaaatcggg cgtgggctgg ctgcctattc cggtggttca cgggatgacg
     4621 ctcggtgaac tggcgctgat gaccaacggc gaacgctggc ttccggcttc gcgcgtgtgc
     4681 gatgtaacgg ttattccctg caaaaactac acgcatcaga ccaggtacgt gctgcccgtt
     4741 ccaccttcgc ccaatctgcc ggacatgaaa tcgatttacc tctacccttc cacatgctat
     4801 tttgaagcta caccggtgag tctgggacgg ggaacggata agccatttca ggtttacggc
     4861 catccaaata tgaagggtta cgacttcact ttcacaccac gaagcatttc gggtgctaaa
     4921 aatccaccac aactcgacag actttgctac ggtgtggatt taagcggaaa aagcgatgaa
     4981 gaaatttggg aaaaaggaat tgatttgagc tatctgattg atgcttacag gaatttgaat
     5041 ttggacgatc attttttccg tccgtttttc gaactcttaa ttggtaatga aaatatccgg
     5101 aaaatgatta aatccggaaa atctgccgac gaaatcaaag caagctggca ggaagatgtg
     5161 gcgcgattca gggaggagcg gaaaccgtat ctggtttata gtgagtagtt tatagtttat
     5221 agtttttttt ttggaaactt aaatttttca ctttgattga aaattagcat ttagcagtaa
     5281 aatagtttat agtatgaaat atcgagattt taaggattta atcgcctatc aaaaaggatt
     5341 tactcaggca atgaatattt ttcaactgtc tgctggtttt cctgttgaag aaaggtactc
     5401 attaaccgac caaatcagac gttcatctcg ttctatctgt acaaacatgg cagaagcata
     5461 tcgtaaaaga gcctatccga aacactttca ttcaaaaatg actgattgtt gggcagaaaa
     5521 tggcgaaacc ggagtttggt tagattttgc atatcactgc aattatattt cagaagaaat
     5581 ttacaatcaa cacattgcat taaacacaga aattggcaaa ctcataacat atatgataaa
     5641 caatcccgaa aaattcggag taaaagaaga ttagctcaac aacaatataa ctatctcacc
     5701 ataaactatc ttactatttt actttaaact atcttactat aaactaaaaa aatgtcacca
     5761 cttgcagtca tcatcaccat agcagcctat tttttggtgc tttttaccgt ttctcacctg
     5821 gcaggacgca aagcggataa tgccggattc ttcatcggca accgcagctc gaagtggtat
     5881 atggtggctt ttgcaatgat tggctccacc atttcgggtg ttacctttat gtcggtgccg
     5941 ggcatggtcg acccgaaggc attttcgtat atgcagatgg tgctcggctt tttcgtgggc
     6001 caactgatta tttcgtttgt gctgattcct gttttttacc ggatgaagct gttttccatt
     6061 tacgaatacc tggacgagcg atttggagtg agtacttaca aaaccggcgc ctggtttttc
     6121 tttatttcca aggtgttggg cgcatcggtg cgactgtttt tagtttgtct ttttctgcaa
     6181 atgatggttt tcggaccgct caatatcccc tttattttca atgtggtagc aactgtggcg
     6241 ttggtttggc tttatacgcg tcagggcgga gtgaaaacgc tgatttggac cgattcgctc
     6301 aaaactttct gcctgattct ttccgtggtt ctgaccatat tttttattgt aaaaaacatg
     6361 ggattggatt tcagcggaat ggtttcaagc atccgcggaa gcgacctttc acgcactttc
     6421 tttttcgata atccgaaaga gggtacatat ttctggaaac agtttctggc agggattttc
     6481 accatgatag ccactacggg acttgatcag gactttatgc aacgaaatct cagttgcgaa
     6541 aatccgcgcg actcgcagaa aaacatcatt accagcggtt tggtgcagtt ggttatcaac
     6601 ctgctgtttc ttatgctcgg cgtgctgctt tacctgttta tcgacagcaa aggcattaca
     6661 cgccctgaaa aaagcgacga acttttcccg atggtagcca cgcagggtta tttaccgctg
     6721 gcggtgggaa ttctatttat tatcgggctg atttcttctg catacgcggc agcaggctcg
     6781 gcactcacag ccctgacaac gtcctttacg gtagatattc tggggataaa aaataaatcc
     6841 gaagtacaaa tcaaatctac ccgtaaaaat gtccatatcg gtatggcagt ggtgatgggc
     6901 gtggttattt ttgtatttaa cttattgaat aacacgagtg taattgatgc cgtttacata
     6961 ctggcaagtt acacctacgg cccgattttg ggaatgtttg cctttgggat tttgataaaa
     7021 cgctcagtaa gagataaatt tgtgccgtat atcgcggttt tagcaccgat aatctgcttt
     7081 ttcctgcaaa aattgacaat agctgtgtgg gatttccact ttggatatgc gttgattatt
     7141 gtaaatgcct tgattacgat ggcgggaatg ttcagcctat ccctaaatcc ctttccaaag
     7201 gaaagggact ggcagagtaa aaagtaagaa tacttactaa tagaaataaa gaatatacag
     7261 aaaaaatgaa aaatacaggc aaaatacata aatcactgcg taaaatcccc cttgggggaa
     7321 acaacgttcg acgtaagcca attagggggc ttttatttgt atttttcttt ttccccttca
     7381 ttctttctgc ccagcttctc acacccgatg ccactcaaaa catcgagaat tatctgaatt
     7441 cggaagtgag caaggagatt tccgttggga aaatccgcat tgacagcact aaaattgcag
     7501 gaaatgaact gcaccttttc attaacggaa atttttccaa ttttccgttc cgaaaccaca
     7561 atgtagcaaa attcaattta gaaattttta aattattaaa cttagaatta accaaatatt
     7621 cactgaaaat atttgcagga acaaaaccgg ttgaagaact gattttgcct gaatttacca
     7681 ctaaaagaga taaaaaccga aaaactttca gcacccatac cgaaattccg ataatcacga
     7741 gattatctgc cccttacaag cctgtaaacg gactacaaaa ccgccacatt gcgctctggc
     7801 aaagccacgg ctactactac gagcagaaac tcgcccgctg ggaatggcag cgcgcccgaa
     7861 tttttcaaac cgtagaagac ctctatacac agagttacgt gctgccatat ctggtaccaa
     7921 tgctcgaaaa tgcgggtgcc aacgtgctga tgccgcgcga acgcgacacg caaaccgaag
     7981 aaatcattgt ggacaacgac gaaggaattc atgatacatc catttattca gaaaaaaacg
     8041 gaaccaaaag ttggtcaaca gggaatgcac ccggttttgc tcagttaaga aaacagtatg
     8101 ttgattttga aaatccattc aaagacggaa ctttccgcca aacagaaacc agtaagaaag
     8161 gcacgaaaac caccgcagaa tggacacctg acattccaaa agcaggaaaa tacgccgttt
     8221 atatttctta ccataccctg ccaaaaagca ctgaggatgc ttattacacc gtttttcacc
     8281 ggggtggaaa atcggaattc agcatcaacc agaaaatggg aggcggcacg tggatttatc
     8341 tcggaacttt cagttttgaa aaaggtaaaa gcggtaaagt ggttttgtcc aatatctcat
     8401 ccaaagccgg tcagatactc actgccgatg gtgtgaaatt cggcggaggg atgggaaata
     8461 ttgcccgcaa agtttcggca gacggagcta ccgaaaacgt gccgagttca cgaagcaacg
     8521 aaccgcaggc gaaaatcgaa aatccaaaaa ttgattatcc gtacgaaaca agcggatatc
     8581 cgcgctacac ggaggctgcc cgctactgga tgcaatgggc gggcattccg gacagcattt
     8641 actccgaaag caaaggccga aacgactaca ccgacgacta caaatgccgc ggactttggg
     8701 tgaattatct ggcgggcggc tcggcagtca atcccctgca aaaagggctg aacatccccg
     8761 tggatatggc tttcgccttt cacaccgatg ccggaacaac ccagaacgac agcataatcg
     8821 gtacactggg catctatttt accggtaccg aaaccggaaa atacccgaac ggcgtatcca
     8881 gaaatgttgc gcgcgagctc accgaccgag ttaattcatt cattgtaagg gatattcggc
     8941 aactttacga gcccgattgg acccgacgtg gcatgtggga tcgctcctac ttcgaagccc
     9001 gctctccaaa cgtgccgact atgttgctgg aactactttc gcaccaaaat tttgccgata
     9061 tgcgttacgg gctcgatccg cgcttccggt ttacggtgag ccgagccatt tacaagggaa
     9121 ttcttcagtt tattgcttcg caaaattaca tgaattacgt cgttcagcca cttccggtgg
     9181 ataatatgag cttgagattt accgggggaa atgaagtgga attaagnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnngaac tggatcacgg agtgccttat ctcaatgatt
     9361 ttaaatacat tgggaaacag aaagaatttc gccggaaaat tccctggatg gacgacgatg
     9421 cctcaggttt tggcgacagc tacgccaatt acgaaacgac tgttatagcc ggcaacacat
     9481 ttgattatcc ggccgttcac ggcgaagcca ttttgaaagc gggctattcg tttgtttcct
     9541 gcagcgacga agccgttgaa aatcaaacgg ttcgattatc ggattacaaa attattgatt
     9601 tgattttagg aaaagaatat cagaccagaa tgggtccggg cggaataaat cagcttcaat
     9661 ttaagacatt caacaccaaa atacagcaag caatttctga atattgccaa cagggcggaa
     9721 atatttttat ttcaggagct ttcgtaggca ccgacttgtg ggacaaccgc tacactgttt
     9781 cgattgaaga ggataagcag ttcgcaatca acattttgaa atacaaatgg cgggcgggac
     9841 aagcaacacg tattggcaat gtaaaagctg taacttcgta ttttccgatg tttgtcggca
     9901 tttataattt ttacaatgaa ttaaatcctg ataattacgt agtggaagct cccgatgcca
     9961 tagagcccgg atcggagggc gcatatacca tttttcgcta ttctgaaaat aacctgagcg
    10021 ccggcgtggc atacaaggga aattacaaat cgtgtgtgct cggattcccg tttgaaagtt
    10081 tgaaaattca gatagaaaga gaaaaaatga tgaaaggagt actggagttt tttgaagccc
    10141 cctaaccatc ccgaaacaaa ttcgggacag attctcaaag ggggaaaatt tcccgtaggc
    10201 gataaatatc aatagcagga taaaaaaata aaatattccc cggaggggat aaatatcaat
    10261 agcagggtaa aaaaaggaag aacattcccc gtagggataa attatcacaa tgaaactcaa
    10321 actaaccata ttatttgtaa tttcccaaat cggtgttttg tacggggtgg agcccttcca
    10381 tttcgcactt ttgactgatt tacatatcac acaaaacaca accgcagtag aagatttgga
    10441 aaattcgttg aaacaaataa ataatacacc tgaaattgaa tttgtactgg ttacgggcga
    10501 tgtaacggag tttggtgata aggtttcgct ggaaaaggcg aaagagcttc tcgacaaatt
    10561 gaacataccg tattacatca cttcgggcaa tcacgaaacg aaatggagcg catcgggaca
    10621 taccgatttt gcacggattt tcggggatga taagtttgct tttgaacaca aaggagtgcg
    10681 gtttttcgga tttaataccg gaccggtttt gcgaatggcc gatggacatg tttcaccaca
    10741 ggatatcacc tggttgaaag atgaattgag taaaaaggag aaaacagagc cggtaattct
    10801 tgctacccac tacccgcttc aatacggcga tgtggacaac tggtacgagc tgaccgatgc
    10861 ggtgcggacg cataacattc gggcgatttt gggcggacat tatcaccgaa acgctgtatt
    10921 tgcctatgat ggaattcccg gcttgattaa tcgctctaac ctccgtgcaa aagccgacgt
    10981 aggcggatat tcggtttact cggttactcc cgatagtctg atagcttttg agcagataat
    11041 tggtggagag ccaaaacgct gggtggccct ttcgatgaaa gagcaatatt tcgacgaaaa
    11101 agggagcgac aggaaatatc ccgatttttc ggtcaaccaa ttttttccga atgtaaagga
    11161 aaaatggctc atgaaaacgg gaactggaat atacagttcg cctgcgatat ggaaaaagaa
    11221 cgttttcgtg ggcgatgatt tgggtaaatt aacaagctac aatctaaaaa acggtaaaaa
    11281 gcaatggagt ttccaaacag gacaaagaat tgtgggaaca cctgcagttg ctgatggcat
    11341 tgttgttttt ggctctgccg ataaaaatat ttacggagta aacgccaaaa ccggcaagca
    11401 aatttggaaa attccgacaa atgaagccgt tctgggaaca gttacgattg aaaacggtat
    11461 tgcgtatatt ggcggaagcg acaattcgtt cagagccatc gaagtaaaat cgggaaaagt
    11521 gaactggatt ttcgacaaac tgaaaggcta tgtggaaaca aaaccactga taatcaataa
    11581 taaagtaatt tttggagcct gggatacttt tttgtatgct ttggagaaaa ataccggaaa
    11641 tctcgtatgg caatggacaa acggcatcaa aagcacgcat tattcacccg ctgcggtttg
    11701 gccggtttct gcgaatggga aagtattcgt tgtggatccc gaacgtgcga tgacagccat
    11761 cgaccttgaa accggcaaaa ccagctggcg aaccaaacaa tcgatggtgc gtgaaagcat
    11821 cggcatttcc gaagatggca accgcattta cgctaaaacc atgcaggaca gcgtggtttg
    11881 ctattcatcg caaagcgaaa cgccccagaa aatctggtcg ggcaacatag gttacggtta
    11941 cgaacataac ccttcaatgc taatagaaaa gagtgaaatt gtatatggaa gtaccaaaaa
    12001 cggacttatc tttgcattgg aagcattgac cggaaaggta atctggaaac ataaaattgg
    12061 caattcgttg gtgaataccg ttttgccgac aggcaaaaaa accgtgctgg taacgacggc
    12121 gagtggggag gtgagcctgt taaaatacac cccctaaccc tttaagggga ataagaaata
    12181 caatttatca aaaaatgcga aataaaataa gtataaataa atcgaacaca aattacacaa
    12241 aacacacaaa tttttaaatt gagaaaatcg tgtttattta agaaattcgt gaaattggtg
    12301 ttcaaaaaca aacacataac attatgacaa aattcagtaa ctccccttca gggattggag
    12361 gccgctcaat taattgcttt attccttacg aggaggctgc acaggtgact cagaccattc
    12421 aaaacctgaa aaaatcaaca ctggttgata aaatttattt agtggcaact actcaacttc
    12481 cggctgtaga aggttgtgaa gtgattgaaa ccaagagtct taaatcaaca gatgctatcc
    12541 aaaaaatagc tgctaaagcc gatgcggatt acacgatgat ttacaccaaa accaccacac
    12601 tgaaatgggt gaattactcc attgaacgga tggtggctat tgccgaagat tcaaacgcag
    12661 ccatgaccta ttccgaccat ttcaaagaag ccaacaacgt tcgccaggaa gcacctgtga
    12721 tcggttatca gatgggttcg ctgcgcgacg attttgactt tggttccgtg ctgctttatc
    12781 gcacatcggc actgaaagaa gcagttcaac gaatggacgc tgactacgac ttcgccggat
    12841 tatacgattt acgtctgaaa gtttcacaaa aaggcgacct ggtacatatc aacgaattcc
    12901 tgtattatga aatagaaaac gacacccgaa aaaccggaga aaagatattt gattatgtcg
    12961 atccgaaaaa ccgcattgtt cagattgaaa tggagcaggc ctgcacacaa catctgaaag
    13021 atatcggcgg atatttggaa ccaaacttca aacacattga attcaccgag gataattttg
    13081 aaatggaagc atcggtgatt attccgtgta aaaaccgcgt aaaaaccatt ggagatgcca
    13141 ttcagtcggc cttaaaccaa aaaaccacct ttccttacaa tgtgattgtg gtggatgata
    13201 attccgaaga cggaacggtg gatgtcatca aaaaattcat ggacaatcca aaactgattt
    13261 acattgcgca ggatacatcc taccatgcaa taggcggcaa ctggaatgca gcgtttcatg
    13321 atcccaggtg tggaaaattt gcccttcagt tagacagtga cgacctgtat tccgacgaaa
    13381 atacggtcca aaaattcgtg gatgcgtttt acgaacagaa ctgcgccatg gtagtgggta
    13441 cgtataaact gaccaatttt gacctggaaa tgattccgcc cggagtgatt gaccacaaag
    13501 agtggacacc cgacaacgga agaaacaacg ccttgcgcat caacggatta ggtgctcccc
    13561 gaggttttta tgtacccatg cttcgtcaaa tcaatttccc gaccacaaaa tacggagaag
    13621 actacgcagt gggattacgt gtgagccgcg aatatcaaat cggacgaatc tacgatgtga
    13681 tatacctttg ccgccgctgg gacgacaatt cggatgcttc gcttgatgtg gtgaaaatga
    13741 acggacacaa cacgtacaaa gaccggatca ggacctggga attgcaggca agagtgaaga
    13801 tgaataaaac ccccaacccc taaaaggggg caacgagtgc aaatggtcaa tatttaatcg
    13861 tttttatttt tatgtgttta ggcaacaatt ctactttatg atatctattt ctatgcaaga
    13921 accaaaactt tctgttggaa taatgtttga atcgaaaatt gatttttcgt tgctaaatgc
    13981 ttatttatgc aatggaaagg agttttttgg taatcaaa
//
LOCUS       DAORKP010000046        13815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_384_length_13815_cov_9.020858, whole genome shotgun sequence.
ACCESSION   DAORKP010000046 DAORKP010000000
VERSION     DAORKP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_13815_cov_9.020858"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLN75_04035"
     CDS             <1..395
                     /locus_tag="PLN75_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRG03320.1"
                     /translation="VPLSYGGILLMHFTDKVHFSTQFLIFWGLMVVLVQLLDYYVPIW
                     GTKKFGGSKRGIWGCAIGMVVGLFFGPWGIVLGPFVGAIAGELSGGKQTQAAIKAGFG
                     SFIGLLLGIVSKLVVGGFLLYYAFVAVF"
     gene            complement(458..823)
                     /locus_tag="PLN75_04040"
     CDS             complement(458..823)
                     /locus_tag="PLN75_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRG03321.1"
                     /translation="MTEIINEQQYEVAMQRIEELLPLVDDNTPTNDRNYIELDMISNL
                     VSDYEEKYFPIEKPGFIDILKLRMYEMGLTQKAMSELIGVSPSRLSNYLTGKSEPTLQ
                     VARRISKELNISADIVLGV"
     gene            complement(826..1122)
                     /locus_tag="PLN75_04045"
     CDS             complement(826..1122)
                     /locus_tag="PLN75_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRG03322.1"
                     /translation="MRVVSKKMLIDYFEAHPQAKTALSEWFEKTREANWRSFADVKKS
                     FNSVDNVGNKRYVFNIKGNDFRLVVLIVFTPKTVYIRFVGTHAEYEKIKNIQNL"
     gene            1398..4649
                     /locus_tag="PLN75_04050"
     CDS             1398..4649
                     /locus_tag="PLN75_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRG03323.1"
                     /translation="MLNIYRASAGSGKTFQLTKDYIFLLFQSLTNNPRPHRRILAVTF
                     TNKATDEMKSRILQELHLLSSGSKSEYRADLMKEFKLTENEVNSRAGKILIRILHDYS
                     SFSISTIDKFFQQVVRSFAREIGLSGGYNLELDTDTTLRQAVDNLYSDMSLKENEQLL
                     SWMTDFLKEQIEDGKSWKVDNEIINFGKEIFRENYQHKADEIRDKLHNKEFLKNYRKK
                     LRKIITDFRENVASSAQETLNYLLDNDLRPEYFMRGIMHSTLEKFKKDDYNFGVTFSK
                     FIDGPENCYKKSEKAEIKSAIDNLYFRGLQPRLINLINLIAEGLILYNSAKIILRHLG
                     TLGILSDLAGQIKKLTGEQNIMLLSDTNLLLNKIIDDSETPFVYERTGVNIDHYMIDE
                     FQDTSVLQWKNFKPLISNSLAANGTDMVVGDVKQSIYRWRNSDWKLLDKQVGKDFSNL
                     QTNQKTLDTNWRSDRNIVHFNNSFFKNASALLQEKLNNNIISATPGNEWSEALSNVIT
                     HAYSDVGQQVSGNASEGHVKIEFIDKEGLKSEWEEAVLKKIPELMENLVDNGYKPKDI
                     AFLVRNREHATTLINYLLTYKKSPNARTGFSYDMVGNEGLKINTSPSINFIISVLKLI
                     FNPYDDTTRTIMLYEYQIGKLKRNEDESVRSCFTEQEIYKTAQSSLFTAEENACMEEI
                     SHLSLFEATEKLISVFGLEKWYSETVFLQAFQDVVFKFSNSHNADLNSFLRWWDENGE
                     KQFITVPENEQAFRIMTIHKSKGLDFKVVIIPFCNWELDSSAKKSTLLWCETSVAPFS
                     ELPLMPVQYSKMLENTIFRNEYFDEQMHQYVDNLNLAYVAFTRARNEMYCFCTKPESK
                     KNNEFSLSTVSDLLYACATSNPDFEQAWKSEDNILETGKPQKAVYKENKANSIIRQLT
                     SYPVTDSSERLKIRHTSADYWKTRPVSESRVNYGTLMHEIMQKTIRRGDEERALATML
                     SSGKINDDDLAEIRKELERFWSLPEVNNWFSEDSEVLNECAILLPSGEMYRPDRILLK
                     NNTATVIDYKFGEEEHTAHHRQLQRYAELLRRMDYTEINSYLYYVSLGKLIEC"
     gene            4836..5183
                     /gene="rplS"
                     /locus_tag="PLN75_04055"
     CDS             4836..5183
                     /gene="rplS"
                     /locus_tag="PLN75_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRG03324.1"
                     /translation="MDLIKVAEQAFDQEKNFPLFKSGDTVTVGYRIKEGNKERIQEFR
                     GDVIKIVGHLNKKRFTVRKMSGNVGVERIFPMDSPFVESITVNKRGKVRRAKLYYLRA
                     LTGKKARIKERRY"
     gene            5360..6100
                     /locus_tag="PLN75_04060"
     CDS             5360..6100
                     /locus_tag="PLN75_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG03325.1"
                     /translation="MGYLYQTGQYALLMQKVFVKPDRWRMFWRQFPKELEKLGMESLP
                     IVIIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDAIEIMGVNSANYLILPKITAFVLMMPFLVIFSMAIGLFGGYL
                     VGMFSDIISVADYLLGIQYAFIPYYIFYSIVKSVVFAFIISSVAAYYGYFSYGGALDV
                     GKASTNAVVNSSILILFFNLLITNIMLN"
     gene            6103..6861
                     /locus_tag="PLN75_04065"
     CDS             6103..6861
                     /locus_tag="PLN75_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG03326.1"
                     /translation="MIEVKNINKSFDGNQVLFDISGVFENGKCNLIIGESGSGKTVLM
                     KSIIGLHRIDKGRIEYDGRNLPDMRMKDIRILRREVGMLFQGSALFDSLTVMENVMYP
                     LEMFSADTRKDMEERARFCLKRVNLEEKAYSLFPSEISGGMQKRVAIARAIALNPKYL
                     FCDEPNSGLDPKTSLIIDRLIQEITQEYNITTIINSHDMNSVMEIGDNIMFIDKGKKA
                     WEGSNKMIFKAENEPLNDFVFASELYKQVKKANS"
     gene            6978..7757
                     /locus_tag="PLN75_04070"
     CDS             6978..7757
                     /locus_tag="PLN75_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRG03327.1"
                     /translation="MKIISYNVNGIRAAMSKNLTGWMKETNPDVLCVQETKAQPEQIE
                     TAELNEMGYFCFVHSAHRKGYSGVAIFTRIPPDNIVAGMDLHKYDSEGRVLRADFDDL
                     TVVTVYIPNGSSTGERHHFKMDFLADFQHYISTLRETRPNIIVCGDFNIAHKPVDIYD
                     PESHEDTSGYLPEERAWLNEFESSGMADSFREFDKNGGNYTWWDMRTFARGKNLGWRI
                     DYLWVSQPLKNRLESAGILSDAFHSDHCPVMLQVTNISTPV"
     gene            7892..9292
                     /locus_tag="PLN75_04075"
     CDS             7892..9292
                     /locus_tag="PLN75_04075"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HRG03328.1"
                     /translation="MKFFQYKNFLFFLGIVIFGVSCGITESQVANPSSDASVLTAYIQ
                     YNDSSPNASKAVFTINNVDSTIYNVDSLPYGTRLDSLYLGFTFASSLGYVMNDTVAES
                     YYYGTAVTSKAYDLTNPAKIKNLASDGESTRQYTIEVRVHKVETYLHVWSELKKYVTN
                     APAENQKAVMLKNKFYYFHNYGTHYALYTSADAANWTQQAAPATLPPDAELRNMLVYR
                     DEILLLYNGNELYKSADGINWQKHTLTADPDYNYQSLLFQFKNKLWAVAQHKTNKSVR
                     ISSSSDGITWTLAEKRSFTNFPVRDFSVTKFKPSLGREKVIVLGGVDASGKTLNTRWS
                     AEDVLGVDTLYWVNLQHKTYKMDQVKQAATAYYGSKLLIIGGSKPTGLLVDAKEQLRQ
                     SIDEGLTFAVPDSTQNLMPDEYPLRTNASLIHDTANNTLYVIGGKTNTSTLADVWKIK
                     VNFYNFKDYLENPAKY"
     gene            9389..9685
                     /locus_tag="PLN75_04080"
     CDS             9389..9685
                     /locus_tag="PLN75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03329.1"
                     /translation="MNKEITIGHVAASICEKSLFKNNRLPKVFQAFVSGNISATIGED
                     EEQLACFSEEFLSYTHTSTATADSPVGTKIKKTIHLYITGWKKAIVFIPTFFIF"
     gene            9771..11612
                     /locus_tag="PLN75_04085"
     CDS             9771..11612
                     /locus_tag="PLN75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03330.1"
                     /translation="MDLEEVEISADRTQLYSSLGRIVTTIEKVQIEQAAVRSIDDLLD
                     FVAGIDVRQRGANGVQADLSIRGGSFDQILVLLNGVNITDPQTGHYNLDIPVDIGDVS
                     RVEILQGSSARVLGPNAFSGAINIVTNENHESKLSTSLSAGSYGFLSQNVSGNYASEK
                     FKMLGSVSHKKSNGHIANTDFDIKNGYFQTGIRTSNAGNINMQLAYQQKSFGANSFYS
                     LTYPNQYEHTKTLFSSVGWELNTGKMSFRSHVYFRRHHDRFELFRDFEGADKFPWYKG
                     HNYHQTDVTGGKFVFSNTWKQGKSTLGVDIRNEHIFSTVLGETIQKPRPVPFENGIDF
                     TREANRMLSTVFTDHSLSLKHWYIAAGAALTHNVKFGTSINGGIDASYNPSDYTRLYV
                     AANSAVRLPTFTDLYYQSATQISNPNLKPEKSLTLELGTKINPINQTFTATTYYRFGS
                     NIIDWVKTAEAEKWESRNLTSVNAMGTDLSYEYRFRNKFLKTAGISYSYLHLNKKAEA
                     YDSKYALDYLKHKFVMKIDHAIWSKLTGHWSFAWHDRSGNYFDFGSKQNTDYKPYTML
                     NAKIMWKTRKMTLFTEINNLLNQHYVDFGGILQPGITVNTGIRLTIF"
     gene            11816..12391
                     /locus_tag="PLN75_04090"
     CDS             11816..12391
                     /locus_tag="PLN75_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRG03331.1"
                     /translation="MKSIKGTQTEQNLLKSFAGESQARNRYSMFAAIAKKEGFEQIAA
                     IFTETADQEYMHAKKFFRYLEGGMVEITAMYPAGIEGTTAENLKASADGENEEWADLY
                     PHFAEIADAEGFSNIAATWRKIAAAEKGHEERFRKLYDRVVNGEVFKREEETDWQCRV
                     CGHIHHGKEAPKACPTCAHAQAYFEEMKTNY"
     gene            12507..13217
                     /gene="mnmD"
                     /locus_tag="PLN75_04095"
     CDS             12507..13217
                     /gene="mnmD"
                     /locus_tag="PLN75_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRG03332.1"
                     /translation="MQKSELIITEDGSHTLFVSEIDECYHSTKGAIQESRHIFIEAGL
                     KQCEKSEINVLEIGFGTGLNALLTAHLTLPPNPLKGEIEVQKVNYSSIELYPIPLEQV
                     LNLNYPETLEKQRNVPPGVFRSLFEKIHTSRWNEKVKITENFTLTKLKADFTKIELDE
                     KFDVIYFDAFSPEKQPEMWSEKMFRKLYLCASENAIMTTYCAKGAVRRAMKEAGFTVE
                     RLPGPPGKREILRAKKIV"
     gene            13289..>13815
                     /locus_tag="PLN75_04100"
     CDS             13289..>13815
                     /locus_tag="PLN75_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRG03333.1"
                     /translation="MFTPKVPLGGFRGLSYFLTPYILFFIVVLAALVHYPKAELHLLM
                     NQNHTSAADWFFRIWTEFGGNIIPFVIIFLFLFYRYSFSLFLLASKLTGVVIFSVAKL
                     IFTEPRPLYYFQIHFPQISLPTVEGVRMLNDYSFPSGHTTSAFAIFFGLSLLVRNKWL
                     KLTFFIVAALVGYSRV"
BASE COUNT         4354 a         2952 c         2829 g         3680 t
ORIGIN      
        1 gcgttccgct tagttacggc ggcattttac tcatgcattt caccgataaa gtacactttt
       61 ccactcaatt tctgattttt tggggattaa tggtcgtttt ggttcaactg cttgactact
      121 acgtcccgat ttggggaacc aaaaaattcg gaggaagcaa gcgaggcatc tggggctgtg
      181 ctattgggat ggtggtcggg ctgtttttcg gtccctgggg cattgtgctc ggacctttcg
      241 tgggcgccat tgcaggcgaa ctttccggtg gaaaacagac tcaggctgcc atcaaagccg
      301 gatttgggtc gtttatcggt ttactgctgg gaatcgtttc aaagctggtt gtcggtggat
      361 ttttgttgta ttatgctttt gttgctgtat tctaaattat ctttgtcaaa gttcaaaact
      421 ttgacaaaga ttacattttt gaaccgcagc tttcacatca cactcccaaa acaatatcag
      481 cgctgatatt caactcttta ctaattctac gggcaacctg aagtgtcggt tcgcttttgc
      541 ctgtcaaata attacttaat cgcgagggac ttactccgat taactctgac attgcttttt
      601 gagttaagcc catttcgtac atgcggagct taagaatatc aatgaagcct ggcttttcga
      661 tggggaaata tttttcttca taatcagaca ccagatttga aatcatatcc aattcaatat
      721 aatttctgtc attagtcggt gtgttgtcat ccaccaaagg caagagttct tcaattcgtt
      781 gcatcgccac ttcgtattgc tgttcgttta ttatttcggt cataattaca aattttgaat
      841 gtttttaatt ttttcatatt ctgcgtgagt acccacaaat ctgatatata ccgtttttgg
      901 cgtaaaaaca attaagacca ctaacctgaa atcattacct ttaatgttga agacataccg
      961 cttattacct acattatcca cgctattaaa cgactttttt acgtcagcga acgacctcca
     1021 gtttgcttct ctggtctttt caaaccactc tgacaatgct gtttttgcct gtggatgtgc
     1081 ttcgaagtaa tctataagca tttttttgga tacaactctc atacagaata tttttcaaca
     1141 aaaatagcac cgatatttca tattacaaaa taatatttca atttttagaa ttattatttg
     1201 aagaaagtta ttgttgagta atgaaaatag tttatttttg taaaaataca ttttcgacaa
     1261 tttaatctta caaaatgcgg attcaaaccg cacattacta ttaaaaagtc cccctcaaac
     1321 tattttcctt tttctttctc catttggaga aagtacccga agggggatag aggtggggga
     1381 tttagggggc ttcttttatg ctaaacatct atcgcgcttc tgccggatca ggcaaaacat
     1441 ttcagttgac aaaggattat attttcctgt tgttccagtc gctgacaaat aatccgcgtc
     1501 cgcaccgccg tatcctggcg gtaacattta ccaacaaggc taccgacgag atgaaatcgc
     1561 gcatcctgca ggaacttcac ctgctttcat cgggcagtaa atcggaatac cgcgccgatc
     1621 tgatgaaaga attcaaactg acagaaaatg aagtgaactc tcgggcaggg aaaattctga
     1681 tacgcatcct gcacgattat tcctcgtttt ccatcagcac cattgataag ttctttcagc
     1741 aggtggttcg ctcatttgcc cgcgaaatag gcctcagcgg cggttacaac ctggaactcg
     1801 atactgacac cacgctccgg caggctgtgg ataatcttta ttcggatatg tcgctcaaag
     1861 aaaacgaaca actgcttagc tggatgaccg attttctgaa agaacagata gaagatggga
     1921 aaagctggaa agtggacaac gaaataatca atttcgggaa agaaattttc agggaaaact
     1981 atcagcacaa agccgatgaa atacgcgata aacttcacaa caaggaattc ctgaaaaatt
     2041 acagaaaaaa actcagaaaa atcattaccg acttcaggga aaatgttgca tcatcggctc
     2101 aggaaacctt aaactacctg cttgacaacg acttaagacc tgagtacttc atgcggggaa
     2161 tcatgcacag caccctggaa aaattcaaaa aagatgatta caactttggg gttacttttt
     2221 caaaattcat cgacggcccg gaaaactgtt acaaaaaaag tgaaaaagct gaaattaaat
     2281 cggctattga caacctttac ttcagaggtt tacaacctcg tctgatcaac ctgatcaacc
     2341 tgattgccga aggattgatt ttatacaact ctgcaaaaat aatactccgg catttgggca
     2401 cactcggtat tctctccgat cttgccggcc agattaaaaa actgaccggc gagcaaaaca
     2461 tcatgttgct ctcggatacc aacctgttgc tcaacaaaat catcgacgac agcgaaacac
     2521 ctttcgtgta cgagcgaaca ggcgtgaaca tcgaccatta catgattgat gagtttcagg
     2581 acacctcagt tcttcaatgg aaaaatttca aaccgctgat ttcaaacagc ctggctgcca
     2641 acggcacgga catggtggtt ggcgatgtga aacaaagcat ttaccgctgg cgaaattccg
     2701 actggaaact actcgacaag caggttggca aggatttctc caacctgcag accaatcaaa
     2761 aaacactgga caccaactgg cggagcgacc ggaatattgt acacttcaac aattcgtttt
     2821 tcaaaaacgc atcggcctta ttgcaggaaa agctaaataa caacatcatt tcggctacac
     2881 cgggcaacga atggtcggaa gcactgagta atgtcattac acatgcatac agcgatgtcg
     2941 ggcagcaagt atcgggcaat gcttccgaag ggcatgtaaa aatagagttt atcgacaagg
     3001 aaggcttaaa atccgagtgg gaagaggcag tgctgaaaaa aatacctgaa ttgatggaaa
     3061 atctggttga caatggatac aagcccaaag atatagcatt tcttgtcaga aacagagaac
     3121 atgccaccac attaataaac tacctgctga cttataaaaa atctccaaat gcccgcaccg
     3181 gattcagcta tgacatggta ggcaacgaag ggctgaagat aaacacttca ccttcaatta
     3241 atttcatcat ttcggttctg aaactgattt tcaatcccta cgatgatacc actcgaacaa
     3301 tcatgctgta cgagtaccag attggcaagc tgaaacgaaa tgaagacgaa tcggtacgct
     3361 cttgttttac tgagcaggaa atatacaaaa cggctcagtc atcgcttttc acagccgaag
     3421 aaaatgcttg catggaagaa ataagccatc tgtcgctctt tgaggctaca gaaaagctga
     3481 taagcgtatt cgggctggag aaatggtaca gcgaaaccgt ctttcttcag gcatttcagg
     3541 atgtggtttt caaattttca aacagccata acgccgattt gaacagtttt ctccgatggt
     3601 gggatgaaaa cggggaaaaa cagtttatca ccgttcccga aaacgagcag gcatttcgga
     3661 taatgaccat ccacaaatcc aaaggtcttg attttaaggt tgttatcatt ccgttctgca
     3721 actgggaatt ggattcttct gccaaaaaat caactctgct ctggtgcgaa acttcagtag
     3781 ctccgttcag cgagctgcct cttatgcccg ttcagtacag caaaatgctt gaaaacacca
     3841 ttttccgcaa cgaatatttc gatgaacaaa tgcaccagta tgttgataat ctgaatctgg
     3901 catacgtggc gtttacgcgt gccagaaacg agatgtactg cttctgcaca aagcctgaat
     3961 caaagaaaaa taatgaattt tccctttcca ccgtttcgga tttgctgtat gcatgtgcaa
     4021 cttctaatcc cgattttgag caggcatgga aaagcgaaga caacattctc gaaaccggaa
     4081 aacctcaaaa agcagtttat aaagaaaaca aggcaaacag catcatccgg caactcacct
     4141 cctaccccgt taccgacagc agcgaacgac ttaaaatccg ccacacaagc gccgattact
     4201 ggaaaacgag acctgtttca gaaagccggg tgaattatgg cacactcatg cacgaaatta
     4261 tgcaaaaaac catccggcgc ggcgatgaag aaagagcact ggccacgatg ctttcaagcg
     4321 gaaaaataaa cgatgacgac ctggccgaaa tccgaaaaga actggagcgt ttctggtcgc
     4381 ttccggaagt aaacaactgg ttttcagaag attcagaagt actcaacgaa tgtgctattt
     4441 tacttccctc gggcgagatg taccgtccgg accggatact gttgaaaaac aatactgcta
     4501 cggtaattga ctataaattc ggcgaagaag aacatacagc ccatcacagg cagctgcaga
     4561 gatatgcaga attactccgc cggatggatt acacagagat caactcatat ctgtattacg
     4621 taagtttggg aaaactgatt gaatgctgat tcataaaaaa acctcacaac taacggaata
     4681 acagccggaa tttttctaaa aaatagttcg aatcattttg ccgtataaaa ataaagctgt
     4741 acttttgcag tccaaatttc agcgtaatct ctggcagcgg gcaaaataag tctgtgaata
     4801 ttgcgcacag tatcaacttt aataaaaatc aaatcatgga cttaataaaa gttgcagaac
     4861 aagcattcga tcaggaaaaa aactttcctc tgttcaaaag cggcgacacc gtaaccgttg
     4921 gttaccgcat caaagaagga aataaagaac gtattcagga attcagaggc gatgtaatca
     4981 aaatcgtagg tcatctgaac aaaaaacgtt ttacagtacg caaaatgtca ggaaacgtgg
     5041 gcgttgaacg tatattcccg atggattcac ctttcgtgga aagcatcacg gtaaataaaa
     5101 gaggtaaggt tcgccgcgcc aaactgtact acctgcgtgc acttaccggc aaaaaagccc
     5161 gtatcaaaga aagacgttac taatcctcac cgaaggaaaa gaaatagaaa agagagattc
     5221 agcattgagt ttctcttttt tgttttccgg aactcctttt caggctccgc gagtgaaaac
     5281 gtatagctac tcaggcgcat gtttgttcgc gattttgttt tatctttgta tttccaaaaa
     5341 aaaaagaaac attacactta tgggatatct ctatcaaacc ggtcagtatg cattgctcat
     5401 gcaaaaggta tttgtgaaac ccgatcgctg gcgaatgttc tggcggcaat ttccgaaaga
     5461 actcgaaaaa ctgggcatgg aatcgctgcc tatcgttatc attatttctg ttttcatcgg
     5521 agctatcatg actattcaga cgaaactgaa taccgagaat ccgctgctgc ccacctacag
     5581 caccggactg gtaacacgcg acacgttgct gctcgaattt tcgtccacca tcatgtgtct
     5641 gattctggca ggcaaagtgg gctcaaacat cgcttcagaa ataggcacca tgcgtattac
     5701 cgagcagatt gacgccatcg aaatcatggg agtgaattcg gccaactacc ttatcctgcc
     5761 caaaataacg gcttttgttc tgatgatgcc ttttctggtt atttttagta tggctatcgg
     5821 ccttttcggc ggttatctgg tgggaatgtt cagcgatatt atttctgttg cagattatct
     5881 gctgggcatt cagtatgctt ttatccccta ttacatcttt tattctattg tcaaatcggt
     5941 cgttttcgct tttattattt catcggttgc ggcctattac ggctactttt catacggcgg
     6001 agcgctggat gtaggaaagg ccagcaccaa tgcggtagtc aacagcagca tactgatttt
     6061 gtttttcaac ctgctcatca ccaacataat gctgaattaa cgatgattga agtaaaaaac
     6121 atcaataaat catttgacgg aaatcaggta cttttcgaca tttcgggcgt ttttgaaaac
     6181 gggaaatgca acctgataat cggcgaaagc ggttcgggaa aaaccgtgct catgaaaagc
     6241 attatcggac tgcacagaat agataaaggg cgaattgaat acgacggacg aaatctgccc
     6301 gatatgcgta tgaaggatat tcgtattttg cgtcgcgagg ttggaatgct ttttcagggg
     6361 tctgcattat ttgattcatt aaccgtgatg gaaaacgtaa tgtatccgct tgaaatgttt
     6421 tcggccgaca ccagaaaaga tatggaagaa agagccagat tctgcctgaa aagagtgaat
     6481 ctggaagaaa aagcatacag cctttttccc tccgaaataa gcggcggaat gcagaaacgg
     6541 gttgccatag ccagagctat tgccctgaac cccaaatacc tgttttgcga cgagcccaac
     6601 tcgggccttg atccgaaaac ttcacttatc atcgacaggc ttatacagga aattacgcag
     6661 gaatacaaca tcaccaccat tatcaattcg cacgacatga attcggtgat ggaaatcggt
     6721 gataatatca tgtttatcga taaaggaaaa aaagcctggg aaggaagcaa caagatgatt
     6781 ttcaaagccg aaaatgagcc acttaacgac tttgtatttg cctccgaact ctacaaacag
     6841 gtaaaaaaag cgaacagtta atgttctgat gtttgggcgt ttaattgttt ggatgtttga
     6901 atgtttgggc gtatataggt atagtttgat tttatgtctt ttttctgaaa gtctaagttc
     6961 tgaaagttta taaaaaaatg aaaataattt cctacaacgt aaatggcatt cgggcggcaa
     7021 tgagtaaaaa cctgaccggt tggatgaaag aaacaaatcc tgacgtgctt tgtgtgcagg
     7081 aaacaaaagc tcaaccggaa cagatagaaa ctgcagaatt gaatgagatg ggatattttt
     7141 gtttcgtaca ttcggcccac agaaaaggtt acagcggagt tgccatattc accagaatac
     7201 cgccggacaa catagtagca gggatggact tacacaaata cgattcggaa ggacgggtgc
     7261 tgcgggcaga ttttgacgac ctgactgtcg tcaccgttta catccccaat ggctcctcaa
     7321 cgggcgaacg gcatcatttc aaaatggact ttctggcaga ttttcagcac tatatttcca
     7381 ctctcagaga aacccgaccg aatataatag tttgcggtga ttttaatatc gctcataaac
     7441 ctgtagacat ttacgacccg gaatcacacg aagacacctc aggctatctg cccgaagaac
     7501 gagcatggct caacgaattt gaatcatcag gaatggcaga ttctttccgc gaatttgata
     7561 aaaatggcgg caattacacc tggtgggata tgagaacctt tgcaagaggg aaaaacctgg
     7621 gctggcgaat tgactatctt tgggtgagcc aaccacttaa gaaccggctt gaatcggccg
     7681 gaatactttc ggatgctttc cattccgatc attgtccggt gatgctgcag gtaacaaata
     7741 tcagcactcc tgtatgaata aaactgcggc acaaattgct tttcgattga taaaaaataa
     7801 taaatcttac tgattaaaga ttagttagtg caaattgttt ttaactttgc atattattta
     7861 catttaagct ttatttatta aacaaccggt aatgaaattt tttcaatata agaattttct
     7921 tttctttctg ggcattgtaa tatttggtgt atcatgcggt ataactgaaa gtcaggtagc
     7981 caatccctcg agcgacgcat cggtgttaac cgcttacata caatacaacg acagctcacc
     8041 caatgcctca aaagccgttt ttaccattaa taacgttgac agcacaatct acaacgtgga
     8101 ctcactccct tacggcacca ggctggatag tctttacctt ggatttacct ttgccagctc
     8161 gctcgggtat gtaatgaacg acaccgtagc agaatcctac tactacggca ctgcagtgac
     8221 cagcaaagct tacgatctca ccaatcccgc aaagattaaa aacctggcaa gtgatggcga
     8281 atcgacccgg caatacacca tcgaagtacg cgtacacaag gtggaaactt accttcatgt
     8341 gtggagtgaa ttaaaaaaat acgtaaccaa tgcacctgcc gaaaatcaaa aagcagtgat
     8401 gctgaaaaac aagttttact attttcataa ttacggcaca cactacgccc tgtacacttc
     8461 tgccgatgct gccaactgga ctcagcaggc tgcacctgca acgctcccgc cggatgccga
     8521 attgcgcaac atgctggttt acagagatga aattttactg ctttacaacg gcaatgaatt
     8581 atacaaatca gctgatggga ttaactggca aaaacacacc ctgacggctg atcccgatta
     8641 taattatcag tcgctgctgt tccagttcaa aaacaaactt tgggctgtag cacagcataa
     8701 aaccaacaaa agcgtcagaa tatcaagttc ttctgatgga attacctgga cactggcaga
     8761 aaaaagaagc ttcaccaatt ttccggtacg cgatttctct gtcacaaaat tcaaaccctc
     8821 attggggaga gaaaaggtaa ttgtgctggg aggagtagat gcaagcggaa aaacgctcaa
     8881 taccaggtgg tctgctgaag atgtattggg agtagataca ctgtactggg tgaatctgca
     8941 acataaaaca tacaaaatgg accaggttaa acaagcagca acagcttatt atggcagtaa
     9001 attactgata ataggtggaa gtaaaccgac cggcttactg gtggatgcaa aagaacaatt
     9061 gcgtcagtct atcgacgaag gactgacttt tgctgtgccc gacagcactc aaaacctgat
     9121 gcccgacgag tacccactcc gcacaaacgc atctctcata cacgatacag ccaataatac
     9181 actttatgtt ataggtggaa aaaccaacac atcaacactt gcggatgtat ggaaaattaa
     9241 agtaaatttt tacaatttca aagactacct cgaaaatccg gccaaatact aagacagttt
     9301 tccgattctg aaaagaacat taaaaatatc gtccaataac attcggttca gacaatggag
     9361 ccggaagagt tatgctgttt tctcggggtt gaataaagaa atcaccatcg ggcatgtagc
     9421 tgcaagtatc tgcgagaagt cactttttaa aaacaaccgt cttccgaaag tatttcaggc
     9481 gttcgtttca ggaaatattt ccgcaacaat cggtgaggat gaagagcaac ttgcatgttt
     9541 ttcagaagaa tttctatctt acacccacac atcaactgcc acagccgata gtccggtcgg
     9601 cacaaagatt aaaaaaacaa tccatttata cattacgggt tggaagaagg caattgtctt
     9661 cattccaacc ttttttattt tttaaaaaaa tcatgattag aagaaacatt gctttactgt
     9721 tttcaatttg cttgttatca ggtatttatt cacaaacaag tgtaaaaaat gtggatcttg
     9781 aagaagtaga aatcagcgcc gaccgcaccc aactatattc gagcctcgga cgtattgtta
     9841 ccaccattga aaaagttcag attgagcaag ccgccgttcg gagcatcgat gatttgcttg
     9901 atttcgtggc cggaattgac gtgcgtcagc gcggcgcaaa cggtgtgcag gcagacctca
     9961 gcatacgagg cggatcattt gaccagattc tcgtattact caacggtgta aacatcaccg
    10021 accctcaaac aggccattac aacctggaca ttcctgtaga tatcggggat gtcagccggg
    10081 tggaaatact gcagggctca tcggcccggg tactgggtcc gaatgcattc agcggagcca
    10141 tcaatatagt aaccaacgaa aatcatgaaa gcaaactctc aacttcgctt tcggcaggaa
    10201 gttacggatt tctatcccaa aacgtgtcag gaaattacgc atcggaaaaa ttcaaaatgc
    10261 tgggttctgt atctcacaaa aagagcaacg gacacatcgc gaatacagat ttcgatataa
    10321 aaaacggcta ttttcagacc ggaatcagaa catcgaatgc aggaaatata aatatgcagc
    10381 ttgcttatca acagaaatca ttcggagcca acagtttcta ttcgttgact tatccaaacc
    10441 agtatgaaca tactaaaacc ttgttttcga gcgttggctg ggaactgaac accggaaaaa
    10501 tgagtttccg gtcacacgta tatttccgcc ggcatcacga tcgtttcgag ctgttcagag
    10561 attttgaagg agccgacaag tttccctggt acaaaggcca caattaccat caaacggacg
    10621 ttacgggcgg aaaattcgta ttttcaaaca cctggaaaca gggcaaatcg acactcggag
    10681 ttgacatacg aaacgaacat attttcagca ctgtactggg ggaaaccatt cagaaaccac
    10741 gaccggttcc gtttgagaac ggtattgatt ttacaagaga agcaaaccgg atgctttcaa
    10801 ccgttttcac ggaccatagt ctgagtttga aacactggta catagcagcc ggagcggcat
    10861 taacacacaa cgtgaaattc ggcaccagca tcaatggcgg tatcgacgct tcgtataacc
    10921 cctcagacta cacgaggctg tatgtagcag cgaattctgc cgtcagattg cccacattca
    10981 ccgatttata ttaccaaagc gccacccaga ttagtaatcc gaatctgaaa cccgagaaat
    11041 cgctgacttt agaacttggc accaaaatca atccgataaa ccaaacattc acagcaacca
    11101 cttactacag atttggaagc aacatcatag actgggtaaa aacagcagaa gctgagaaat
    11161 gggaaagccg gaacctgaca agcgtgaacg caatgggaac agacctgagc tatgaatacc
    11221 ggttcagaaa taaatttctg aaaactgccg gaataagtta ttcttacctt catctgaata
    11281 aaaaagcaga agcctacgac tccaaatatg ccctcgacta cctgaaacat aaatttgtaa
    11341 tgaaaataga ccatgctatc tggtcaaaac tcaccggtca ctggagtttt gcctggcatg
    11401 accgcagcgg aaattatttt gatttcggat cgaaacaaaa cactgattac aagccatata
    11461 cgatgcttaa cgccaaaatc atgtggaaaa cccgaaaaat gactcttttc accgaaatca
    11521 acaacctgct caatcaacat tacgtcgatt tcggaggcat cctgcaaccc ggaattacag
    11581 taaataccgg tatcagacta accatttttt aaaaaaatga acctatttag aatgaaatta
    11641 aattaatgaa agcagtaatc aagtaataaa ctgaatgctt gacttttaag atttcggata
    11701 caagtaaaat ccgaatattc catcatctaa actgctgaaa tagtttttgt tatccttaca
    11761 ttttattaat tttgtaaata agaaaacaca ttttataaat taaatattta tctctatgaa
    11821 aagtatcaaa ggaacacaaa cagaacaaaa cctgttgaaa tctttcgcag gtgaaagtca
    11881 ggcacgcaat cgttacagca tgtttgctgc cattgccaaa aaagaaggtt ttgaacaaat
    11941 agcagctatt ttcaccgaaa cagccgacca ggagtatatg cacgccaaaa aattcttccg
    12001 ttacctggaa ggcggcatgg tagaaattac cgccatgtat cccgcaggca ttgaaggcac
    12061 taccgctgaa aatctgaaag catctgccga tggtgaaaac gaagaatggg ctgatttgta
    12121 ccctcacttt gcagaaatag ctgatgccga aggcttttca aatatcgctg ccacctggcg
    12181 caaaatagcc gcagccgaaa aaggtcacga agaacgcttc cgcaaacttt acgaccgagt
    12241 agtaaacggc gaagtgttca aacgcgaaga agaaaccgac tggcaatgcc gcgtctgcgg
    12301 acacattcat cacggtaaag aagctccgaa agcctgcccg acatgtgctc atgctcaggc
    12361 atatttcgaa gaaatgaaaa caaactacta ataaaattga cgatttaaca atttgcgatt
    12421 tacgattgta aattgtgttg taattcattg aaaatccctt tcttttaagt aagggatttt
    12481 ttattttaaa taattacctt tgcacaatgc aaaagtcgga actgataatt accgaagacg
    12541 gttcacacac gctgtttgta tctgaaattg atgaatgtta ccattccacg aaaggggcca
    12601 ttcaggaaag caggcatatt ttcatcgaag ccggattgaa gcaatgtgag aaatcagaaa
    12661 tcaatgtgct cgaaatcggt ttcgggacgg gacttaatgc gcttttgaca gcccatctaa
    12721 ctctgccccc taacccccta aagggggaaa ttgaagtgca aaaggtaaac tattcgtcga
    12781 ttgagttata tccgattccg ttggagcaag tattaaatct gaattatcct gaaacgttag
    12841 aaaaacaaag aaatgttcca cccggagtat ttagaagcct tttcgaaaaa attcacacat
    12901 cacgctggaa tgaaaaagtt aagataacgg aaaacttcac cttaacaaag ctgaaagccg
    12961 attttactaa aattgaattg gatgaaaagt ttgatgtaat ctatttcgat gctttttctc
    13021 ccgaaaaaca gccggaaatg tggagtgaga aaatgtttag aaagttgtat ctttgcgcgt
    13081 cagaaaacgc cattatgaca acctattgtg ctaaaggagc tgtaagaagg gctatgaaag
    13141 aagccggatt tacggtagaa cggcttcccg gacctccggg gaagagggag attctgagag
    13201 caaagaagat tgtctgaacc atgatttctt tgattattat gatttatatt tgaaaaaaaa
    13261 cacatcacta aatttaaaat atatttttat gtttacacca aaagtccccc ttgggggatt
    13321 tagggggctg tcctattttc tcacccctta cattttattt ttcatcgtcg tgctggctgc
    13381 tttggttcat tatccaaaag cggaactcca cttgctgatg aatcaaaacc acacttctgc
    13441 agccgattgg tttttcagaa tctggacaga attcggagga aacattatac cctttgtaat
    13501 aatttttctt tttttgtttt atcgctatag tttttcattg tttttattgg catcaaaact
    13561 aacaggtgtg gttatttttt ctgttgctaa attgattttc accgaaccac gcccgctcta
    13621 ttattttcaa atccacttcc cgcagatcag tttacccacc gtagaaggtg tcagaatgct
    13681 caatgattat agtttcccgt cggggcatac cacatcggct tttgccattt ttttcggatt
    13741 gtcgttgctg gtcagaaata aatggttaaa actcactttc ttcatcgttg ccgcattggt
    13801 aggatattca cgcgt
//
LOCUS       DAORKP010000047        13757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_387_length_13757_cov_9.662093, whole genome shotgun sequence.
ACCESSION   DAORKP010000047 DAORKP010000000
VERSION     DAORKP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_387_length_13757_cov_9.662093"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1263
                     /locus_tag="PLN75_04105"
     CDS             151..1263
                     /locus_tag="PLN75_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRG03334.1"
                     /translation="MSEKWVTLAIRTYQRAQMIKAVLEQNGIETVIHNLNLENPEMAV
                     GVRVRIKEDDLPQALSIVEDKEKAWEEETRKNEPPKGRNILIPIELTDQINEVCKYGF
                     YFAKKINADVVFLHTYLSPAYTISSSYSTEINTYSLADTEMLRRIVRSNSADVENLTN
                     LVNRWIETGEIPKVKFSFELREGVPEEEILSYCKKESPSLVVMGTRSRFQKDENLIGS
                     VTAEVLEACKAPVMAISLGLKMLPEKIRRIAFLTNFDQKDLIAIDTAISFLNKDHLEL
                     VFIHATQKKESWDEVMLAGIKEYFANHYPNLKTEYALLDKEKNLEQIQPFLEASKIDL
                     VALNTRKRSLFSRFFNLGIATRLLFNTDTPLLVMHL"
     gene            complement(1362..2510)
                     /locus_tag="PLN75_04110"
     CDS             complement(1362..2510)
                     /locus_tag="PLN75_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRG03335.1"
                     /translation="MQKINLFLPFVLIFTFSVSTLANNDTIPEKNKSLEIHASYTGDI
                     LTNVVGGIQQKTAYLGMLNLEINLNTTYANWWKGGNFYVKATNTHGESPSANIIGDLQ
                     TVSNIEAGNHTFLQEAWFSQSVSNLNIIFGLQDLNKEFAVTKYGSSFINSSFGIIPTI
                     SCNLNAPIFPLTSVGVTLKWEINEKLSWLNAIYDGEPTDFAENPFNTNWQLHANDGLL
                     AISEVQVNDNKPLPATYKIGLYNHGHSLSKLIYKNPPTITHNSWGAFAYADKNAWKKN
                     NQSFGLFAQAGYGFSAKSNCKMYLGGGFNLSGLIKKDGSDLIGLAIAYAKLEENKQNE
                     TAIEFTCHFPLNDYFYIHPDIQYIINPSGIDQNLPNSLAGIIRIGAQL"
     gene            complement(2624..3214)
                     /locus_tag="PLN75_04115"
     CDS             complement(2624..3214)
                     /locus_tag="PLN75_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRG03336.1"
                     /translation="MRQIHIAISDESLVIREGLQSVFSRYQFIHRISHAPTLDALEKL
                     LQRTTIQIVIINPYVIQSNQSQFLQLKNKNECIKWIGMLYSHFTSGMLAKLDGTIDIF
                     DTGEAIAAKLLKWIESGSTENSLQSKEILTERETDVLKHLADGKSNKEIADKLNISIN
                     TVITHRKNISQKTGIKSVSGLTIYAVVQNLISLDNF"
     gene            complement(3211..3936)
                     /locus_tag="PLN75_04120"
     CDS             complement(3211..3936)
                     /locus_tag="PLN75_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03337.1"
                     /translation="MKKTNKILPDIKIAEAIIDNPFLVLLLERFNISLPVQDLNISDL
                     CKSYGINETLFEAFVSMYNGNTSIPELKLNEHDALTIVHFLRVSHIYYSEQIYPEITE
                     LINRMNQLNDHKEMKLVSVFFKDYFREVTDHLDYENEVFHPYVIQLASQINNNEPSNI
                     RDEYSVKQYKARHDDIEEKLNDLRNLLIKYLPVNQDQTVRRKLLFLLSELEFDLKIHS
                     DIEELILIPFTLRMEKKVKQTEQ"
     gene            complement(3966..4907)
                     /locus_tag="PLN75_04125"
     CDS             complement(3966..4907)
                     /locus_tag="PLN75_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRG03338.1"
                     /translation="MKNFKIVSYNVENYFDVVDDSLTSDEEYLPTGIRAWNYSKYTTK
                     QNNIAKVLAAIGGWEAPAIVGLCEIESEKAMRDLTLYSPLKGLGYKYIHHESPDARGV
                     DVALMYQPHQFKPIHDEIIRIPSFKTRDLLYVSGKTTNEDTLHIFVCHFPSRLGGELE
                     SEDRRVTVAEKLRQKVDSVFINSGNANIVIMGDFNDYPSNSSITEILKAIPPTDNFQP
                     ESLYNLASVLQSRGKGSHKFQGEWGMLDQMIVSGNLLNPQNKISTTTSDMHVFEPDFL
                     LEPDETFLGKKPKRTYNGMAYQGGFADHLPVMLDLWY"
     gene            complement(5057..6535)
                     /locus_tag="PLN75_04130"
     CDS             complement(5057..6535)
                     /locus_tag="PLN75_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRG03339.1"
                     /translation="MSTKIFQKIIIIVAGGLFFMLTAHSCIDEQISADPALKLTFSKD
                     TLRFDTIFTTIGSSTSQIKVYNPNNKNVEISAIGLASGAASPFRINVDGALNKDNRFT
                     NIELRAKDSLYIFVEVTVDPLDVNSPVFIKDSIVFLTNNNQQDVKLIAYGQNMEVLRN
                     TTFSTDTTLTAEKPYLIYGNLVVDTAKTLNLLPGCRLFFHDQSSLMVYGNLIADGTRE
                     KPILMRGDRTDQIFEDVPYNLVSNQWGGVLLLSKEGNHVFNFVKMNSGYVGIYFSNDD
                     RNFRPKLTISNSRIHNFLKYGLVVQNGDVLVVNSEISNTGAYSVYLNGGKHTFIHSTI
                     ANYFNSTNVRIQPSGKEGNAAVMIMDLNRIIPMETVFKNCIVAGSNSGEFEILTRFES
                     QYHGSFSNSYIRKEKPDPVPAMFTNIIWYNPKDTIFKNTFYDLNKLEYYNFIPDSVSP
                     ARNIGNPDVAKLYPVDLNGNSRLTDNMPDAGAYEWIPTKKSR"
     gene            6780..7058
                     /locus_tag="PLN75_04135"
     CDS             6780..7058
                     /locus_tag="PLN75_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03340.1"
                     /translation="MASKRKLKKIMQFISSELITDVFFKTLMSGKEATEKADLLVVEI
                     SQFTREHINRIGRNGGKENPKEVKEYYRKLYTDWNNGIEKYIDEIGKL"
     gene            complement(7212..7883)
                     /locus_tag="PLN75_04140"
     CDS             complement(7212..7883)
                     /locus_tag="PLN75_04140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRG03341.1"
                     /translation="MGVFIALLVIISWASHLAYMLLLQPVNLLDVWFWVHMLIQTWLF
                     TGLFITAHDSMHGTISANRKLNNVIGFSATLLFAGMWYPMLIKKHKMHHVNPGTALDP
                     DYYTGKQNFFIWWFSFMKSYVTIWQILIMAGLFNLGLLFFSEIQLIVLWIIPSILATF
                     QLFYFGTYLPHRLPHTTEMGIYKSRTLKKNHLWAMLSCYFFGYHYEHHASPQTPWWKL
                     YQTNN"
     gene            complement(7982..9181)
                     /locus_tag="PLN75_04145"
     CDS             complement(7982..9181)
                     /locus_tag="PLN75_04145"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRG03342.1"
                     /translation="MKILVLNCGSSSVKYKLFDMNVRKVVGFGGVEKIGMKGSFLKHT
                     REDGEKVILEGEVLEHQVAIEYILGVLTSKKHGCLNDLDEISAVGHRVVHGGEQFNSS
                     VIITEEVIRKVESCIEIAPLHNPPNLAGIYAISEMLPLIPQVAVFDTAFHQTMPEYAY
                     MYGIPYSLYKKYAIRRYGFHGTSHRYVSQRACEFLGLDYQKTKIITAHIGNGASITAI
                     ENGKSVDTTMGFTPVEGLMMGTRSGDIDLGVVTYLMEKEMIGTSAATTLFNKHSGVLG
                     VSGLSSDMRDIEKAISEGNQRAKLALDMYEYRIKKYIGAYAAALNGFDVLVFTGGVGE
                     NQTATREKICKGLTFMGIEVDSEVNKIRGEEILISTKNSKVKVVVIPTDEEYMIAADT
                     MELTGKR"
     gene            complement(9193..10149)
                     /locus_tag="PLN75_04150"
     CDS             complement(9193..10149)
                     /locus_tag="PLN75_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRG03343.1"
                     /translation="MASPTKEPIERYGLSKRSRKKTLFSKIGVVGCGKDGSIIATAAA
                     THGMEVVFLEPTEEQITNAFRRIEEKLDRRIKGWGLTENEKKVIMSRIKGTMDYSDFE
                     GCDFVIEAIRYNALTGERRIAERKEVFRKLESVLATDAIIASNVTTVIVSDLASELEY
                     KSRCIGLHFLSNVVESQIIEIVRGIETSDETYNIVCKFARLINYQYVPVVESSGLVSI
                     RLFLIQLNEACSMLMEGIASTEDIDRIMNVGFGHRQGLFRIADKLGIEKIVKLMENLY
                     DEYGHVKYKPSPILLRMYRAKHLGVSTGRGFYTYDESGNVIK"
     gene            complement(10190..11191)
                     /gene="pta"
                     /locus_tag="PLN75_04155"
     CDS             complement(10190..11191)
                     /gene="pta"
                     /locus_tag="PLN75_04155"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRG03344.1"
                     /translation="MKLLEEIMQRAKNNLQRIILPEGTEIRTLKAADFILKEKAAKLI
                     LIGNTGEIQQLAAENNLNHISEALIIDPDTYGEMQQYSDLLYELRKSKGMTPEEAVQL
                     AKDPLYLACLMIKNGDADGELAGARNTTGNVLRPAFQIVKTKPGIKIVSGALLMFTPA
                     SQYGEDGLFVFADCAVNPNPTADELAQIAVSTADTARVIAGFEPRVAMLSFSSKGSAQ
                     HELVDKVTEATRIAREMAPHLQLDGELQADAALVPAIGQSKAPGSTVAGHANVLVFPD
                     LQSGNISYKLVERLSGAQAIGPVLQGIAAPINDLSRGCSVEDIIRMITITANQAMKA"
     gene            11477..13621
                     /locus_tag="PLN75_04160"
     CDS             11477..13621
                     /locus_tag="PLN75_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRG03345.1"
                     /translation="MKTTKCILGAMLLTTFFSCNNQLKNASILKQSDFSQPPVAEIKA
                     DTFTRFGVQRIDHYFWMKDKTNPKVIEYLKAENAYTDSVMASTKDFQQKIYDEIIGRI
                     KEDDQTYPTLSNGYYYYTRTEKGKQYRTYCRKKGSLSAAEEITFDVNKMAEGKPAYMF
                     GGNTISPDNSKAAYFYNETGSFADYMLKIKDLTTGNDLDFEVKGASSFAWANDNKTIF
                     YSVIDPVTLRSYRVYRQTLGAGKPELVFEEKDSKFSIGVSASKTREWIIVSSGSATTS
                     EDRILPADNPMGEFNIFLPRVKDVEYDVYPHREYFFIRYKDKENLNGKIYQVAKADYA
                     NRSAWKEIMAHNKDVRIESLNVQKDYLVIETRKNGLTELEIRHLTTGKKRFVSFPEPV
                     YNAYSNGNPEYDASTFRYSYTSLNRPTTLYEYDCTNGNTTLLKQQEIPSGFKPDNYVV
                     ERLWAVAADGAKVPLSIVYKKGLKKNSLNPALLYAYGSYGMSTDVFFSSAYYSLIDRG
                     FVFGIAHIRGGSDLGEQWYEDGKLLKKKNTFTDFIACSEKLIADKYTSADKLAAAGGS
                     AGGLLMGAVANMRPDLYHTIVAQVPFVDVVTTMLDDTLPLTTGEYEEWGNPNEEEYFK
                     YMLSYSPYDNIKAQNYPNMLITGGINDSQVLFHEPTKYAAKLRAMKTDDNILLLKMNM
                     DSGHGGATGRYDGIKETAFELAFILNRVGIEK"
BASE COUNT         3878 a         2738 c         2910 g         4231 t
ORIGIN      
        1 taaatgctgg ttttcaatca aatatagata tttttaaatt tttgtcatgt acttataatg
       61 tttttaatga aaatttaaat ccaactttca gttataattg ttatttttac acacaagaat
      121 tacatttgtc aaacattaat ctgaaaaatc atgagtgaaa aatgggttac actggctata
      181 agaacctatc agcgggctca gatgataaag gccgtacttg agcaaaacgg tatcgaaacg
      241 gtgattcaca atcttaatct ggaaaatccg gaaatggctg tgggtgttcg tgtgaggata
      301 aaggaagatg atttgcctca ggcactcagc attgtggaag ataaggaaaa agcctgggaa
      361 gaggaaacaa gaaaaaacga acctccaaaa ggcagaaata tcctgattcc gattgagcta
      421 accgatcaga taaacgaagt gtgcaaatat ggtttttatt ttgctaaaaa aataaatgcc
      481 gatgtggttt ttctgcacac ctatctgtct ccggcatata ccatctcttc gtcgtacagc
      541 accgaaataa acacctacag cctggccgat accgaaatgc tccgcagaat tgtcagatct
      601 aacagtgccg atgttgaaaa tcttacaaat ctcgtcaacc ggtggatcga aacgggcgaa
      661 atacccaagg tgaaattcag ttttgagctt cgggaaggag tgcccgaaga agagattttg
      721 tcttattgca aaaaagagtc accctcgctg gtggtgatgg gtacccgcag cagatttcaa
      781 aaagacgaga atctgatagg aagtgttacg gccgaagtac ttgaagcctg taaagcaccg
      841 gtgatggcta tttctttggg attgaagatg ttacctgaaa agatcaggcg tatagcattc
      901 cttacaaatt ttgatcagaa agatcttatt gctatcgata ctgccatttc cttcctgaat
      961 aaagatcatc tcgagttggt gtttatccat gccacacaaa agaaagaatc atgggatgag
     1021 gtgatgcttg cagggataaa agagtatttt gccaaccatt atcccaacct gaaaacagaa
     1081 tatgcgttgc tggataaaga gaaaaatctg gagcaaattc agcctttcct ggaagcctct
     1141 aaaattgacc tggtagctct taatacacgt aaaagaagtc ttttttcacg ttttttcaat
     1201 ctgggaattg ctactcggtt gctttttaat accgatacac ctctgttggt tatgcatctg
     1261 tagatcacga taatctaatg ttgatgtact tgcattattg cctcagaaat tttctgttta
     1321 tatctgcaga atgcctgttg gtttgatgtg gttgcaacgg attataattg agctccaatc
     1381 cggattattc cggcgaggct gtttggcagg ttctgatcaa ttcctgatgg atttataatg
     1441 tattggatat cagggtgaat gtaaaagtaa tcattcagag gaaaatggca ggtgaattca
     1501 atggctgttt cattttgctt gttttcctct aattttgcat atgctattgc caatccaatt
     1561 agatcgcttc cgtctttttt tatcaacccg gataagttaa atcctccccc aagatacatt
     1621 ttacaattac tttttgccga aaatccatat cctgcctgag caaaaagacc aaacgattgg
     1681 ttgttttttt tccatgcatt tttatcagca taggcaaatg ctccccagga attgtgtgtg
     1741 atggttggtg ggtttttgta aataagtttt gacagtgagt gcccgtgatt gtacaaacca
     1801 attttatagg tggccggaag aggtttgttg tcattgacct gtacttcaga aatagctaat
     1861 aatccatcat tcgcgtgcag ttgccagttt gtattgaatg gattttccgc aaaatctgtc
     1921 ggttcaccat cgtaaattgc attcagccac gataacttct cgtttatctc ccattttaat
     1981 gtaactccta cggacgttaa tggaaaaata ggagcattca ggttgcatga gatagtgggg
     2041 attattccga atgaactgtt tataaacgaa cttccgtatt tagttacagc aaattcttta
     2101 ttcaaatcct gcaatccgaa aataatattc agatttgata cagattgact gaaccaggct
     2161 tcctgaagaa atgtgtgatt tccggcttcg atatttgata cagtctgtaa atcaccgata
     2221 atattggccg agggactttc accgtgagta ttggtagctt ttacgtagaa attacctcct
     2281 ttccaccagt tggcgtaagt ggtgttcaga tttatttcca gatttaacat tcccagatat
     2341 gcagtttttt gctgtattcc gccaaccaca tttgttaaaa tatcgccggt atatgaggcg
     2401 tgaatttcta atgatttgtt tttttcggga atcgtgtcat tatttgccag cgtagatact
     2461 gaaaatgtaa atatgaggac aaatgggaga aataagttga ttttttgcat gggttgacat
     2521 tgaattaaat accctgcaaa attacacatc aggtaaaaag gctgaaataa tcaaatatta
     2581 tgattttacc tgcttgtatc taaccggaaa tgaggattaa ttatcagaaa ttatccagcg
     2641 aaatcagatt ttggactact gcgtaaatag taagtccgga aacggatttg attccagttt
     2701 tctgagatat gttttttcgg tgagtgatta cagtattgat actgatgttc agtttatcgg
     2761 ctatctcttt gtttgatttg ccatctgcaa ggtgtttgag cacgtcagtt tctctttcgg
     2821 tgagtatttc tttgctttgc aatgaatttt ctgtgcttcc ggattcaatc catttcagca
     2881 gtttggcggc gattgcttca ccggtatcaa atatgtcaat cgtgccatcc agttttgcaa
     2941 gcattccgga tgtaaagtga gaataaagca ttcctatcca ttttatacat tcatttttgt
     3001 ttttcaattg caggaattga ctttggttgg attgaataac atatggattg atgataacta
     3061 tctgaatagt agttcgttgc aacaattttt caagagcatc gagtgtaggg gcatgtgata
     3121 tccggtgtat aaactgataa cgcgaaaaaa ccgactgaag accttcacga atgaccagcg
     3181 attcgtctga aattgctatg tgtatttgtc tcattgttca gtctgtttaa cttttttctc
     3241 cattcgaaga gtgaacggta tgaggataag ttcttcaata tcagaatgaa ttttgagatc
     3301 aaattccagt tcggagagca aaaagagcaa tttacgccgt acagtctgat cctgatttac
     3361 gggtaggtat ttgatgagca gatttctcag atcgttcagt ttctcttcga tatcatcgtg
     3421 acgggctttg tattgcttga ctgagtattc atctcttata ttggaaggtt cattattatt
     3481 tatttgactg gctaactgga ttacgtatgg atgaaacacc tcattttcat aatcgagatg
     3541 atcggtgact tccctgaaat aatccttaaa aaaaactgaa acaagtttca tctctttgtg
     3601 atcgttgagt tgattcatcc ggttaattaa ctctgtgatt tcggggtaga tttgctcgga
     3661 ataatatatg tgtgatactc taagaaaatg aactatagtc agtgcgtcgt gctcattcag
     3721 tttcagctca ggaatggacg tgttgccgtt gtacatgctc acgaacgctt cgaatagcgt
     3781 ttcgttgatt ccgtaagatt tgcataaatc gctgatattc aaatcctgaa caggcaatga
     3841 aatgttaaat cgttcgagca gcagcacgag gaatggattg tcaatgatgg cttcggcaat
     3901 tttgatatcc ggaagtatct tgttggtttt tttcatattt cagtaataaa aaaaatcctt
     3961 aatgtttagt accacaaatc cagcataacg ggcaaatgat ctgcaaatcc accctgatag
     4021 gccattccgt tgtatgttcg tttcggtttt ttaccgagaa atgtttcatc gggttcgagc
     4081 aaaaagtcgg gttcgaaaac atgcatatca ctcgtagtgg tcgatatttt attctgtggg
     4141 ttgagtaaat tgccggaaac gatcatctga tcgagcattc cccattctcc ctgaaattta
     4201 tgtgaacctt tacctctcga ttgcagaacg gaggcaaggt tgtacaaact ctccggctgg
     4261 aaattatcag ttggcgggat ggctttgagt atttcggtaa tgctgctgtt tgaaggataa
     4321 tcgttgaaat cgcccataat gacaatattt gcatttcccg agtttataaa aacactatcc
     4381 actttttgac ggagtttttc ggccacagtt acccgcctgt cttccgactc cagttcgcct
     4441 cccaaacgtg aagggaaatg acacacaaaa atatgcaggg tatcttcgtt ggttgtctta
     4501 cccgaaacgt agagtaagtc acgtgttttg aacgagggga ttcgaatgat ttcgtcgtgt
     4561 atcggtttga actggtgcgg ctgatacatc agtgcgacat caacacccct ggcgtcgggc
     4621 gactcatgat gtatgtattt gtatcccagt cctttcaggg gtgaataaag tgtcaaatcg
     4681 cgcatggctt tttcgctctc aatttcgcaa agtccaacaa tggcaggtgc ttcccaaccg
     4741 ccgatggcag ccagcacctt ggctatgttg ttttgtttgg ttgtgtattt tgagtagttc
     4801 caggcgcgta ttccggttgg caggtattcc tcatcggagg taagcgagtc atccaccaca
     4861 tcaaaatagt tctccacatt gtagctcact attttgaaat tcttcatttc cctgttttga
     4921 cccaaaactg aaattgaaaa aaatagcaat aaaatataca agtaacgggt tttcatattt
     4981 ttcagttttt agaaaaaaaa tatctgacta actcttaaga tgctctaatc tgcaatattt
     5041 tcccttttcg ggagatttag cgggactttt ttgtcggtat ccattcgtat gcaccggcat
     5101 cgggcatatt gtcggtaagc ctgctgttgc cgttcagatc caccggatat agcttggcaa
     5161 catccggatt gcctatgttg cgtgccggtg aaacggagtc gggtataaag ttgtaatact
     5221 ccagcttatt caaatcgtaa aacgtatttt tgaaaatagt gtctttcgga ttgtaccaaa
     5281 tgatattggt gaacatggct ggtaccggat caggtttttc ctttcgtatg taactgttgc
     5341 tgaacgaacc atgatactga ctttcaaaac gggttaaaat ttcaaactca ccggaatttg
     5401 agccggcaac aatacagttc ttaaacaccg tttccatcgg gataatccgg ttcaaatcca
     5461 tgatcataac tgcagcatta ccttctttac ccgaaggctg aatccgaacg ttggtactgt
     5521 tgaaatagtt ggcaatggtg ctgtgaataa aggtatgttt acctccgttg agatacacgg
     5581 aataagcgcc tgtatttgaa atttcggaat ttacaaccaa tacatctccg ttttgaacca
     5641 ccagaccgta tttcagaaag ttatgaatac gggagttgct gatggtaagt tttggccgga
     5701 aatttctgtc gtcgtttgaa aaataaatgc cgacataccc gctgttcatt ttaacgaaat
     5761 tgaaaacgtg gttgccttct tttgagagca gtaaaacgcc gccccactga ttggagacca
     5821 ggttgtaagg gacatcttca aaaatctgat cggtacggtc accgcgcata agaattggct
     5881 tttcgcgtgt accatcggca atcagattgc catacaccat caggcttgac tggtcatgaa
     5941 agaacaaccg gcatcccggt agcaggttaa gcgtttttgc tgtatccacc accagattgc
     6001 cgtaaatcag gtaaggcttt tcggctgtta atgttgtgtc ggtgctgaaa gttgtgttgc
     6061 ggagcacttc catgttttgt ccgtaggcaa tcagtttaac atcctgctga ttgttgtttg
     6121 tcagaaatac aattgaatct tttataaaaa caggagagtt aacatccaga ggatcaacgg
     6181 ttacttccac gaaaatatac aggctgtctt tagcccggag ttcgatgttc gtaaaacgat
     6241 tgtccttgtt caacgctccg tccacgttga ttcggaaagg cgatgcggcg ccactggcca
     6301 ggccgatagc cgaaatctcg acgtttttgt tgttcggatt atatacttta atttgcgatg
     6361 tggaacttcc gatggtggtg aaaatggtat caaaccgcaa tgtgtctttc gaaaatgtca
     6421 gtttaagtgc cggatcggca gaaatctgct catcaatgca tgaatgggct gtgagcatga
     6481 aaaaaagtcc gcctgcaaca atgattatta ttttttgaaa tattttcgtc gacatttttc
     6541 cggtttattc ggtattaaaa ttaacgtagg atctgcattt ttatttccag ccacaaattt
     6601 attcaaaaaa gatgacaaaa cgatttttat ccattatatt tgtgctaaat agcaaatgaa
     6661 gaattacaat gaaatcgcaa tgctgacaga agacagattg ctgcatttca gattttattt
     6721 tcttcaatta tcttttatga tttaaacatc tgatttctaa cgtttaataa aaatatgaca
     6781 tggcaagcaa acgaaaactt aaaaaaatca tgcagtttat ttcatcggaa cttatcaccg
     6841 atgtgttttt taaaacgctg atgtccggca aagaagcaac tgaaaaagcc gatctgttgg
     6901 ttgtggaaat atcacagttt acccgggagc atatcaaccg gataggccga aatggcggta
     6961 aggagaatcc gaaagaagtg aaggagtatt acagaaaact ttataccgac tggaacaatg
     7021 gaatagagaa atatatcgat gaaatcggga aattgtaaaa atacaaaagg acctctccaa
     7081 tgaagtcctt tttttgtttg taagcaacta taaatttgca tttaatcccc ttgtgacggt
     7141 tgctatcgtg tattccccat cgacaggaac cgctacaacg ctataaaaaa gcaaatcacg
     7201 tttttacggg gttaattatt tgtctgataa agtttccacc agggagtttg cggcgatgca
     7261 tgatgctcgt aatgataacc aaaaaagtag cacgaaagca ttgcccacaa gtgatttttc
     7321 tttaatgtac gggatttgta gattcccatt tcggtagtat gcggcaatcg gtgcggtaaa
     7381 taagtgccaa aataaaagag ctggaaagta gccaaaatgg aaggaataat ccagagaaca
     7441 atcagttgaa tttctgaaaa aaacaacaat ccaaggttaa ataatccagc cattattaat
     7501 atttgccaaa ttgtaacata cgatttcata aaagaaaacc accagataaa gaaattctgt
     7561 ttaccggtgt aataatcggg atcgagagcg gtgcccggat ttacgtggtg cattttgtgc
     7621 tttttgataa gcatcggata ccacattccg gcaaaaagca aagtggctga aaaaccgatg
     7681 acattattga gctttcggtt tgctgaaatg gttccgtgca tggaatcatg agcagtaata
     7741 aacagacctg taaaaagcca tgtttgaata agcatgtgga cccaaaacca gacatccagc
     7801 aagtttacag gttgcaaaag gagcatatat gccaaatgcg atgcccagct gattatgact
     7861 aaaagtgcaa taaaaactcc catttttgtt tttttagatt tgttcaattt agaaatgagt
     7921 tcttttttga aaaaaaccta aaaaagtctt cactgagtta gtgactttgt gtgattttat
     7981 cttatctttt ccctgtcaat tccattgtat cggcagctat catgtattct tcgtcggtag
     8041 gtatgaccac cactttcact ttggaatttt ttgtactgat aagaatttct tcaccccgta
     8101 ttttattgac ttcggagtcc acctcaatac ccataaaggt caatcctttg catatttttt
     8161 cgcgggttgc ggtttgattt tcgcccactc cgcctgtaaa cacaagcacg tcaaatccgt
     8221 tgagtgcggc ggcatatgcg ccaatgtatt ttttaatccg gtattcgtac atatccagag
     8281 ccagttttgc ccgttgattg ccttcgctga ttgctttttc gatatcgcgc atatccgacg
     8341 aaagacccga cacacccaaa actccgctgt gtttgttgaa taatgtggtt gctgctgaag
     8401 tgccaatcat ttctttctcc atcagataag ttaccactcc cagatcgata tcgccggaac
     8461 gagttcccat cattaatcct tctaccggtg taaatcccat ggtagtatct accgattttc
     8521 cgttttcaat ggcagttatg gaagcaccgt ttccgatgtg ggcggtgatg atttttgtct
     8581 tttgatagtc cagtcctaaa aattcacatg ctctctgcga cacataccgg tggctggtac
     8641 cgtgaaatcc gtatcgtctg atagcgtatt ttttataaag cgaatagggt attccgtaca
     8701 tgtaggcata ttcgggcatg gtttgatgaa aggctgtatc aaaaactgcc acctgcggaa
     8761 tcaggggaag catttcgctg atggcatata ttccggccag gttgggtgga ttgtgcagag
     8821 gagctatttc gatgcacgat tctacttttc taatcacttc ttcggtgatt attacacttg
     8881 agttaaattg ttcgcctccg tgaaccaccc tgtgtcctac ggcactgatt tcatccaggt
     8941 cattcaggca gccgtgtttt ttgctggtta atactcccag aatgtattca atagccacct
     9001 gatgttcaag tacttctcct tcaaggatca ctttttcgcc gtcttcgcgc gtgtgtttga
     9061 ggaaagagcc tttcatcccg attttttcta cacctccgaa tccaaccact ttccgtacgt
     9121 tcatatcgaa caatttgtac ttaacagacg agctgccgca atttaatact aatatcttca
     9181 tttgctgtcg ttttatttta ttacattacc cgattcatca taagtataaa atcctcttcc
     9241 ggtgctaact ccgaggtgtt ttgccctgta cattcggagt aatataggtg atggcttgta
     9301 ttttacatgg ccgtattcat catacaggtt ttccatcagt tttacaattt tttcaatgcc
     9361 cagtttgtca gctattctga ataagccctg acggtgtccg aatcctacgt tcattatccg
     9421 gtcgatgtct tcggtgctgg ctatgccctc catcagcatc gagcaggctt cattcagctg
     9481 aataagaaag aggcggatgc ttaccaaacc cgacgattca accaccggaa cgtactgata
     9541 attgattaac cgggcaaatt tacacacgat attatacgtt tcatcagagg tttcgatccc
     9601 tctcactatt tcgataatct gagattcgac cacattcgaa aggaaatgca gcccaatgca
     9661 acggcttttg tactccagct cggaggctaa atccgaaaca atgacagtgg ttacgttgga
     9721 agctatgata gcatcggtgg caagcacgct ttcgagtttt ctgaaaacct cttttcgttc
     9781 ggcaattctt cgttcgccgg tgagtgcgtt gtatcggata gcttcgatca caaaatcaca
     9841 tccttcaaaa tcggagtaat ccatcgtgcc tttgatgcgg ctcatgatta cttttttctc
     9901 gttctcggtg agtccccatc ctttgatgcg gcgatccagt ttctcttcta tgcgccgaaa
     9961 tgcattggta atctgttctt cggtaggttc gagaaaaact acttccattc cgtgtgtagc
    10021 agcggctgta gcaattatac tgccatcttt gccgcaacct accacaccta ttttagagaa
    10081 tagtgttttt tttcggcttc ttttgcttaa tccgtaccgt tcaatcggtt cttttgtagg
    10141 tgatgccatt atatattcaa gattttaaaa ttttggaatt cactgtttat caggctttca
    10201 ttgcctgatt agcggtaatt gtaatcattc ggatgatgtc ttcaacggag catccgcgcg
    10261 aaaggtcgtt gatgggtgct gcgatgccct gcaatacagg accgatggct tgtgctcccg
    10321 acaatctttc cactaatttg taactgatat ttccggattg cagatcgggg aatacaagta
    10381 cattggcatg tccggccact gtacttccgg gtgctttgct ctgcccgata gccggaacga
    10441 gtgctgcgtc ggcctgcaat tcgccgtcga gttgcaggtg gggagccatt tcacgggcta
    10501 tgcgggtggc ttcagtcacc ttatctacca gttcgtgctg tgcactccct ttcgaggaga
    10561 aactcagcat ggccactcgc ggttcgaatc cggcaatgac tctggctgta tcggccgtgc
    10621 ttacagctat ctgagccagc tcatcggctg tcggattggg atttactgca cagtcggcaa
    10681 atacgaagag cccgtcttcg ccgtactgac ttgccggagt gaacatcagc agcgctcccg
    10741 aaacgatttt tattccgggt ttcgttttaa caatctgaaa agcggggcga agcacatttc
    10801 cggttgtgtt gcgtgcgccg gccaactcac catccgcatc accgtttttt atcatcaggc
    10861 aggccagata caatggatct ttagccagtt gtacggcttc ttcgggtgtc atgcctttag
    10921 atttccggag ttcgtagagc aggtcggaat attgctgcat ttcaccgtag gtatcaggat
    10981 cgataattaa cgcttcgctg atgtgattga ggttattttc ggctgcgagt tgctgaattt
    11041 ctccggtatt gccaatcaga ataagtttag cagctttttc ttttaaaata aaatcggcag
    11101 ctttcagggt gcgaatttct gtgccttcgg gtaaaataat ccgctgtaaa ttgttttttg
    11161 cacgctgcat aatttcttca agaagtttca taggagctta tttgaattta gattttttgt
    11221 aaatagctga ttttatgaaa ttaaatccat ttggttggat tcttacaaag ttaagtcatt
    11281 tttgttaaaa accaatgtag aaatatcaat tttgtagaaa aatattatgc tcgtaatttt
    11341 tctgtttatt atctcaactc agttttgaaa ttttacatat cctattcata ttattatcgt
    11401 tatgaaaatt atactttggt catttttatt catcagattg attaactttg taaaaaaata
    11461 aaaacaaccg gtaacaatga aaacaacaaa atgtatattg ggagccatgc tgctcaccac
    11521 atttttttca tgtaataatc aacttaaaaa tgccagtatt ttgaaacaat ctgatttttc
    11581 ccaaccgcct gtagctgaga tcaaagctga tacatttacc cgttttggag tacagcgaat
    11641 cgatcattat ttctggatga aagacaaaac caatccgaaa gtgatagagt atctgaaagc
    11701 cgaaaacgct tacacagaca gcgtaatggc ttctaccaaa gattttcaac agaaaattta
    11761 cgatgaaata atagggcgta ttaaggaaga tgaccaaaca tatcctacgc tttccaacgg
    11821 gtattactac tacacccgaa ccgaaaaagg aaagcaatat cgcacatatt gccgcaaaaa
    11881 aggttcttta agtgctgcgg aagaaattac atttgatgtg aataaaatgg ccgaagggaa
    11941 accggcttat atgtttggcg gtaatacaat aagccccgat aacagcaagg ccgcctattt
    12001 ctacaacgag acaggctcat ttgccgatta tatgctgaaa atcaaagacc tgactaccgg
    12061 aaatgatttg gatttcgaag taaagggagc ctcatcattt gcctgggcca atgataataa
    12121 aactattttt tacagtgtga ttgatcctgt aacattgcgc tcataccgcg tttatcgtca
    12181 gacacttggt gccggtaaac ctgaactggt tttcgaagaa aaagattcga aattcagtat
    12241 cggggtttct gcttcgaaaa cacgtgagtg gatcattgtt tcaagcggaa gtgccaccac
    12301 ctctgaagac agaatactcc cggccgacaa tccgatgggt gaattcaaca ttttcttacc
    12361 acgggtaaag gatgtggaat acgatgttta tccccacagg gagtatttct ttatccgcta
    12421 taaagacaag gaaaatctga atgggaaaat ttatcaggta gccaaagctg actatgcaaa
    12481 ccgttcggcc tggaaagaaa taatggctca taacaaagat gtccgcatcg aaagtctgaa
    12541 tgtgcagaaa gattatcttg tgattgaaac acgtaaaaac ggactcacgg aacttgagat
    12601 acgccatctg actaccggta aaaaacgttt tgtttcattc ccggagcctg tttacaacgc
    12661 ctattcaaat ggaaatccgg aatacgacgc ttcgaccttc cgctattcgt acacttcgct
    12721 caacagacct accaccctct acgaatacga ttgcaccaac ggtaacacca cgctgctcaa
    12781 acagcaggaa attccttcgg gtttcaaacc ggataattac gtagtggaaa gactatgggc
    12841 ggtagcggcc gatggtgcaa aagtaccgtt atcaattgtt tacaaaaaag gactgaaaaa
    12901 aaacagcctg aatccggcgc tcctctacgc ttacggaagc tatggaatga gtacagatgt
    12961 ttttttcagt tcagcttatt atagtttgat tgatcgtgga tttgttttcg gaatagcaca
    13021 catacgcgga ggtagtgatc tgggagagca gtggtatgaa gatggaaaat tgctgaaaaa
    13081 gaaaaatacc tttaccgatt ttatagcatg ttccgaaaaa cttattgctg ataagtacac
    13141 ttcggccgat aagctggctg ctgccggtgg aagcgcaggt ggcctgctga tgggtgctgt
    13201 tgccaatatg cgtcctgatt tgtatcacac cattgtggct caggtgcctt ttgtggatgt
    13261 ggtgactacc atgctggatg atacgctgcc gcttactacc ggcgaatacg aagagtgggg
    13321 aaatccgaac gaggaagaat atttcaaata catgctgtcg tactcaccgt atgacaatat
    13381 taaggcgcaa aactacccca acatgctgat tacgggcggt atcaacgatt cgcaggtgct
    13441 attccacgaa ccgacgaagt atgcggctaa actgcgtgcc atgaaaacgg atgacaatat
    13501 ccttttgctg aaaatgaata tggacagcgg acacggcggt gctaccggtc ggtatgacgg
    13561 gataaaggaa acagcatttg aactggcatt tatcctgaac agagtgggga ttgaaaaata
    13621 aaaacaaaca ataagggcat cgattcggtc gatgccctta tttcatattg ttaaccggtt
    13681 ataatttgcc ttttacaatt tcaagaatgg ctttttccat ctcatcagct ttttgttcca
    13741 gttcgtctgc tttagcc
//
LOCUS       DAORKP010000048        13569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_403_length_13569_cov_7.977357, whole genome shotgun sequence.
ACCESSION   DAORKP010000048 DAORKP010000000
VERSION     DAORKP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_13569_cov_7.977357"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..2002
                     /locus_tag="PLN75_04165"
     CDS             302..2002
                     /locus_tag="PLN75_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03346.1"
                     /translation="MAYITVIVTSLLFSECTLNEDLTNLKSSVSDLKLFIGTPEFKTG
                     VHLDFIDAKTNAYITGKALVKISGKDASIIYNNLGEARNSEHQTMNGMLSLIVDPHKV
                     DAFTIGKNPVKFDIIVSLPGYAGVTRSITIQEYKTINIIVPLVKLDDTPEGVTVIENT
                     SFASSSSTGAVQTTSVETLNSGNQTVEVQQNVVLQDESGKPVTGVIQSQVIFYDPTST
                     AAQNAIPGGTSVTATLENGKTQETKLESAGLFDISLKAGDAHVKKFSNGGLKIKTVLS
                     PSLINPITKQTIKAGDEIALWSKQENTGRWIFEKMDIIKNEGGELILEETVTHLSYWN
                     WAWTGTILAEGPKITWKGESMGESVKVSNETGSVITYAKPNDANDGIYQLRNFADVTG
                     TGKTVLLKFSSPMDVSAFSPSSILINAVNQTSNFNVIISRKPSVLELKLKFSASVQKS
                     ANGTPGASISPSFNLNIKPKNSAATDLFTTIPFVSGVGHLNLNLGTEYALNISIGTVT
                     LDGTIKIEEEGSNLKLTAIQNSEPTKPYYYTVPKPADNKIEAKYDISVSQDEMNLLNP
                     "
     gene            2014..2760
                     /locus_tag="PLN75_04170"
     CDS             2014..2760
                     /locus_tag="PLN75_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03347.1"
                     /translation="MKINFLVFFFSIFSLSLLAENNGLTLYADDRSNEKLPYFGVALG
                     VSASTNGLGININTAITKNLSFRIGYESINKTFNNVYTYSIQDQSLTFSPTWQSGGIS
                     ALADFYLFKGLYISGGAIYSNMNLACTVNSGNSIKMGDITFEPDEIGNLNLTIEPLNR
                     LSPYGALGFGSNVSRDKRLALSFELGTYYMRSYLVGLSGTNLLSENGPGNQEALDKLN
                     ETLKTISWSGFYPVVKFGLSYKFYGKMRKQ"
     gene            complement(2931..3515)
                     /locus_tag="PLN75_04175"
     CDS             complement(2931..3515)
                     /locus_tag="PLN75_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03348.1"
                     /translation="MIIRKQFLKTAGISLIILSVMFGCGCFKRNLSTVSNDAGPVKIN
                     DDTGSKGNKGQVLAQAVVYKTSKDYSAFVPVILSADKKEIVSYPAPTDVYYRGKLAYP
                     VALKNGYCLDIRGINEYAAFTDFTYQEYAALKTAPTKEELMKRIIDKHPFQEIIYCGP
                     RANYRNETEELNRLIDEDFKGMKVVKFPKMEVVL"
     gene            complement(3518..6088)
                     /locus_tag="PLN75_04180"
     CDS             complement(3518..6088)
                     /locus_tag="PLN75_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol protease/hemagglutinin PrtT"
                     /protein_id="HRG03349.1"
                     /translation="MFTLKKTSVFLFTILFSITLFARPIDKEEAQNIAKKYFSSGISS
                     SLRMKSAMNFELAYTCTDIADVKNLPQSSYYYVFNIGQNNGFVIVSADSRTKKVLGYS
                     LTGSFDILRIPVNLKAWLNEYANQIRFAIGNLPDVDSETSSPLRTIRKTARQNLTVEP
                     LLGNISYDQGKPYNDSCPALADVRTVTGCGATAVAQVMKYYNWPVKGTGIKAYTSATN
                     HFNLSADFGNTTYDWTNTLPYYSSSGTENSLQKAAIAKLMLHVGISIKMDYNTSANGG
                     SSSYTTDIPPALYNNFGYDAGIQIYQRKYLQYEAWENILITELDARRPVLYRGSTADN
                     AGHIFVCDGYDANGLFHLNWGWGGSSNGYFEISAMNPDGPGIGSGNDGYNLGHYIVTG
                     IQKPVSGSVHASLVAIDSISPSQRTIPRTGSVTLSIKKLMNVGAFDYLSSIMGTALIL
                     SQNGVTIDTLLVDKEWSNNLPPNYYYSALNYDVNFKPSIPNGDYEISAFYRNEHNLFV
                     PVLVNNSGRKYLYAKITSTTIDFISDNSKPELSLVAAPTVLLNLYENKNGRFEMKIKN
                     NGAKDYYAQIGVRITKTDDDEIYQDVIHSITTIPAGETITLQMGDSIKTTPGNYYLNV
                     YYNEANNVGDVSFPTDLMGPSQYTTTVTVNPTPTTAPVMSVVSGTYIVPATITKGQSF
                     TVKANVSNTGGLFDNRMIAFIFLMTPGTSVGYYGHQNQLIDNNQTRSLDFTGEITDLD
                     PGSYRTALYYHTGTSWSRISDIKTFTLVDNPTSVYNLKSDQLRILETPVASELKITTP
                     ADATSLAIFTLQGRAVQSVETRGEVQHSIPVIHLMPGIYILKMAVSDGKIISRKFIKK
                     "
     gene            6547..7032
                     /locus_tag="PLN75_04185"
     CDS             6547..7032
                     /locus_tag="PLN75_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG03350.1"
                     /translation="MQFTKLSGIYRFFVTVFLMAGIVISTDSQTINFWFTNGDSQNYQ
                     LTSASKIQFLNGSLIFNNISTTPIQQIRKITFNTPTEAKEITALQPHPVLSPNPAKDY
                     VIFSNLPAKEEIVSVYSITGAIILTSRISSSSNILDISMLNKGAYVVKTQNFSIRLIK
                     L"
     gene            7029..9260
                     /locus_tag="PLN75_04190"
     CDS             7029..9260
                     /locus_tag="PLN75_04190"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRG03351.1"
                     /translation="MKKTIIILTALIFSVISLVAADYLFIHLKNNTVVALDMTSLDSI
                     AFTSGQSELAVYRNTQSANVYPIAAIDSITFASMTDETIRIIYYGTNVSIINPFTCRG
                     VDVAVSGADVTINSNLSDKEITCSVSGTTTDGSLKIYSNFRHTLALNGADITNTRGPA
                     INIQSRKKCTFLLTDGTTNSLADGITYASDPEDQKSTLFSEGQLIIKGGGTLNILSRS
                     KHAICSDDYIEIEGGTIHVTSAAKDGFHANDHFNLEAGTVTVAATGDAVDCEEGNIKV
                     SGGSITSTIATNDTKGLKSDSTIFISGGIIDFTVSGKQAKGLKSGGNITITGGITNIT
                     CSGGVVLQASGSGSDPSYCTAIKSDKNILISGGDVNINHSGEAGKGISADGNLEISGG
                     RITSNTSGNGNTYTNSSGLMDGYNATCITTNSDLRLIAGDITCSASGTGGKGISAGGN
                     IIVGSSEGNPVVSLTTTGKEISVTSGRTITTYASPKTMKADGDITFNNGTTTIWSSDD
                     GIKSESNVYLYGGNINVNKSYEGIEAPYIYLSGAYADVLASNDAINTTKGTRSGGVET
                     PDGSCLYINSGTLIATCTNGDGIDSNGSIIMTGGTAIVNGPSTGVEEAVDYNDNFNMN
                     GGVFIGAGSNSSMTKSMSVTSTQPNMYVSSGSMISSATLIDIRINSTDVITFKPKYGA
                     YKFLLSTPKMTKEASYTIYTGGSYSSATNTAGLYTGGTYTPGTSKKSGTLSSSGTVTS
                     VSF"
     gene            complement(9371..9877)
                     /locus_tag="PLN75_04195"
     CDS             complement(9371..9877)
                     /locus_tag="PLN75_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03352.1"
                     /translation="MKRKLFFPVLLIALVVAMNSCKLGNDSNYSPRLTLMNPALINTD
                     STLRMSYTNEGNIKFDSLHVNDTVTISVIGEGFTNNLTAFEYEVTNPGDIEVIIPDSI
                     KTFFESRSDFVKGTLYFTPKIVWMNIPFKFVAKKTSDKVKLNFLLESDAKNVSNLYGL
                     QIEFPVKP"
     gene            complement(10011..10083)
                     /locus_tag="PLN75_04200"
     tRNA            complement(10011..10083)
                     /locus_tag="PLN75_04200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10048..10050),aa:Pro,seq:cgg)
     gene            10239..10958
                     /locus_tag="PLN75_04205"
     CDS             10239..10958
                     /locus_tag="PLN75_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRG03353.1"
                     /translation="MIEKAIDNYLSKNHFSELSLKAVFFDMDGVLFDSMPHHSVAWVK
                     AMNESGLEFSEQEAYMNEGKTGTATINGVFVRLNGREATEAEKQDIYRLKAQYFEELG
                     AIGTMPYALEMLKKIKEKGYRIFVVTGSAQPTLLDSLKDHFPGIFEKENIISAFDVTQ
                     GKPHPEPYLKALQKAGVKPWEAVVIENAPMGVQAAVAARIFTIGVNTGPLDSNVLTEN
                     GADLVLHSMQELYEKWSDFGF"
     gene            10977..11990
                     /locus_tag="PLN75_04210"
     CDS             10977..11990
                     /locus_tag="PLN75_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRG03354.1"
                     /translation="MFIKRIITAISLLMIVAGLTASETDSLKKYLPRIEGTIRAKYEY
                     NTSLDASRFQIRSARFSINGSLSPVTSYKAEIDLSDEGETKMLDAYVRLKPVDWFNFT
                     IGQQKIPFGTDNLRSPHQLYFANRSFVGKQLVSGLRDVGATLMFSNRKALPFDFQAGV
                     YNGDGLYNQQDWQKSMNYAFRMEIFPTEYMEISLNYNSVMPDNLRMNLYDVGAYANFS
                     NLHLEGEYIYKTYQGEVFPATKSYSVFAVYDIWTNKRFLKKITPLVRFDSMTDNNKGK
                     PSEGNVYQTDDVASKRLTGGITFSLGKPFQNDIRLNYEKYYYAANHVNSDDKLVLEFV
                     VRF"
     gene            12012..13298
                     /gene="hisD"
                     /locus_tag="PLN75_04215"
     CDS             12012..13298
                     /gene="hisD"
                     /locus_tag="PLN75_04215"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRG03355.1"
                     /translation="MQLIKYPAREQWAELLKRPSLDVASLYDQVKMTLDEVREQGDTA
                     LRNFTLKFDKVDVKVPEVTKEEIAEGVKAVSVELKQAIEMARRNIWKFHSEQQREYTE
                     IQTSPGVYCWQKAVPIEKVGLYVPGGSAPLFSTVLMLGIPAQIAECKEVILCTPPGMD
                     GKIHPAVLYAAQVAGIHRIFKAGGAQAIAAMAYGTESIPKVYKIFGPGNQYVTAAKQL
                     VSLTDTAIDMPAGPSEVEIIADETASAAYVASDLLSQAEHGADSQAILVTTSENLMDE
                     VLRQIEIQIEKLPRKETARKALENSKIILVKSLDEAIAITNEYAPEHLIISTRSYMVV
                     AEHIINAGSVFLGNMSPESAGDYVSGTNHTLPTHGFAKAYSGVNLDSFLKKITFQQLT
                     PDGLTNLSNAIILMAENEGLQAHANAVKVRIEKPEL"
     gene            13331..>13569
                     /locus_tag="PLN75_04220"
     CDS             13331..>13569
                     /locus_tag="PLN75_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03356.1"
                     /translation="MDKKTDSKNSEERLEKAPFWELRGLLRKNIQSLEPYSCARDEFK
                     GEASVYLDANESPYNAPYNRYPDPLQWKLKEKISE"
BASE COUNT         4205 a         2910 c         2835 g         3619 t
ORIGIN      
        1 aatgttgaat cattaaaaaa atgcccgact gtcagatgaa gtcttagcta atacatttta
       61 ttgccgagac agtgcgggtt tcagcgtttc ttcatctaat aaactttcgt gtaacttgac
      121 aggtcagtgc ttcgaaatcg gcaacaaaaa cataccgcca aaccgttatc aatttacacg
      181 catataaatt gggatttcag aaaaagtaaa aaacagtgga tacccgaatc cacaaaaaaa
      241 cataaacgaa cattgtgatt ttgaaataaa aaacgatttt atgaaggggt ataacaaatt
      301 aatggcttac attacagtaa tagttacttc gctgctgttt tcggaatgca cgctgaatga
      361 agatttgaca aatttaaaat cttccgtcag tgatctcaaa cttttcatag gtacaccaga
      421 gttcaaaaca ggtgtacatc ttgatttcat tgatgcaaaa accaacgcct acatcaccgg
      481 taaagctctt gttaaaatat ctggaaaaga tgcatcaatc atttataaca acctgggaga
      541 agcaaggaac tcagagcacc agactatgaa tggaatgctg agtctaattg ttgaccctca
      601 caaagtggat gcattcacca tcggtaaaaa tcctgtaaag ttcgatatca tcgtatctct
      661 gcctggatat gccggtgtca caagaagcat tacgatacag gaatacaaga ccattaatat
      721 tatcgttccc ttagtaaaac ttgatgacac accggagggt gtaaccgtca ttgaaaacac
      781 ttcttttgca tcatcttcat caaccggagc agttcagaca acgagtgtgg aaacattaaa
      841 ttcaggtaat caaaccgttg aggttcagca gaatgtagtt ttacaggatg agtcgggaaa
      901 acctgtaacg ggagtaatcc agtctcaggt aatattttac gatcccacat ccactgctgc
      961 tcagaatgca atacctggtg gaacgagcgt aaccgccaca ttggaaaacg gtaaaactca
     1021 ggaaacaaaa ctcgaatcag ccggtctgtt tgatatatcg ctgaaagccg gagatgccca
     1081 tgtgaaaaaa ttcagcaacg gaggcttgaa gatcaaaacc gtcctttcac cttcccttat
     1141 caatcccata accaaacaaa ccattaaagc aggtgatgaa attgcattgt ggagtaaaca
     1201 agaaaatacc ggcagatgga tttttgaaaa aatggatatc atcaaaaatg aaggtggaga
     1261 actgatactg gaagaaacag tgactcacct aagttattgg aactgggcct ggacaggaac
     1321 gatactggcc gaaggaccaa aaatcacctg gaaaggtgaa tcaatgggcg aatcggtaaa
     1381 agtgagcaac gaaacgggat ctgtaataac gtatgcaaaa cccaatgatg caaatgacgg
     1441 tatatatcag ttgcgaaatt ttgccgacgt gaccggaacc ggtaaaacgg tattactaaa
     1501 attttcaagt ccgatggatg taagtgcctt tagtccttca agcattttga taaatgccgt
     1561 taatcagacc agcaatttca atgtcatcat aagcaggaaa ccaagtgttt tggaacttaa
     1621 attaaaattc tcagcaagcg tacaaaaatc cgctaacgga actcccggtg ccagcatatc
     1681 acccagtttc aatttaaata ttaaaccaaa gaattctgcc gcaacggatc ttttcaccac
     1741 cattcctttt gtatcgggtg taggtcattt aaacctgaat ttaggaactg aatatgcatt
     1801 aaatatatct atcggtactg taaccttaga tggtacaata aaaatagaag aagaaggttc
     1861 gaatctgaaa ttaaccgcaa tacaaaactc agaacccaca aaaccatatt attatacagt
     1921 acccaagcct gccgataata aaattgaagc aaaatatgat atctcggtaa gccaggatga
     1981 aatgaatttg ttgaatccat aatcaattta taaatgaaaa taaattttct ggttttcttt
     2041 ttttctatat tttctttatc acttttagca gaaaataatg gtttaacgtt atatgctgat
     2101 gatagatcaa atgaaaaatt accttatttc ggagttgctt tgggagtaag tgcttccaca
     2161 aacggactgg gaataaatat taatactgcc attacgaaaa acctttcatt tcgtatcgga
     2221 tatgagtcga taaataaaac atttaacaac gtttacacat acagcattca ggatcagtcg
     2281 cttacatttt cgccaacatg gcaaagcgga ggcatttcgg cattagctga cttctatttg
     2341 tttaaaggac tatacatttc gggaggtgcc atctattcca acatgaatct ggcgtgtact
     2401 gtaaattcag ggaactctat caaaatgggt gatattacat ttgagcccga cgaaataggt
     2461 aatctcaacc tgacaatcga acctcttaac aggctttcgc cttatggagc gttaggtttt
     2521 ggaagtaacg tttcacgcga taaacgtttg gctctgagtt tcgaactggg tacatattat
     2581 atgagatcat acctggttgg gctttcagga acaaatttat tatcagaaaa tgggcccgga
     2641 aatcaggaag ccctggataa actgaatgaa acacttaaga ccataagctg gagcgggttc
     2701 tatcctgttg taaaatttgg attatcctat aaattttacg gaaagatgag aaagcaataa
     2761 acttcgtatc aaaaggaatg attttatcaa atgccataag tggattgcct gatataaaaa
     2821 aaaatcattt gacatatgaa acaatttgtc ccgatcagtc ggaatgaaaa ttcattccgg
     2881 caaagcggga caaactgctt aaatctgtat taataaaatt atctggtgga ttaaagcact
     2941 acctccattt tagggaattt taccaccttc atgcccttaa aatcctcgtc tatcagccgg
     3001 tttaactcct ctgtttcatt cctgtaattc gcgcgcggtc cgcaatagat tatctcctga
     3061 aacggatgct tatcaatgat tcttttcata agttcctcct ttgtcggagc cgtttttaag
     3121 gcggcatatt cctgataggt aaagtcggta aatgctgcat actcgtttat tccgcggata
     3181 tccaggcaat acccgttttt cagtgctact ggataagcca gtttgcccct gtaatacaca
     3241 tcggtgggtg cagggtacga aactatttcc tttttatcgg ccgagagaat tacgggaaca
     3301 aaagcagaat aatctttcga ggttttgtaa actacagcct gagcaagcac ctgaccttta
     3361 ttgcctttac tgcctgtgtc gtcatttatt ttcaccggcc cggcatcatt agaaacggtg
     3421 gacagattcc ttttgaagca gccacaacca aacattacag aaagtatgat taagcttatt
     3481 ccagcagttt tcaggaattg tttacggatt atcatatcta tttttttatg aattttctgc
     3541 taatgatttt accgtccgat acagccattt tcaaaatgta aattcccggc attaaatgga
     3601 taaccggaat ggaatgttga acttctccgc gtgtttcaac agattgaact gccctaccct
     3661 gcaaagtgaa aatagctaag gatgttgcat ctgctggagt tgttattttc agttcggatg
     3721 ccacgggcgt ttccaaaatc cggagctggt ctgatttcag gttgtaaaca gaggttggat
     3781 tatcaaccag cgtaaacgtt ttaatatcag aaatccggct ccacgaagtt ccagtgtgat
     3841 aatacaaagc agtacggtaa cttccgggat caagatcggt aatttctcca gtaaaatcca
     3901 gagaacgggt ttgattgttg tcaataagct gattctgatg cccataataa cctaccgatg
     3961 tacccggagt catgaggaaa ataaaggcaa tcatcctgtt gtcaaaaagc cctccggtat
     4021 tggatacgtt agctttcacg gtaaaagact ggcccttggt aatggtagca ggcacaatgt
     4081 acgtgccgct gactaccgac atcacaggag ccgtagttgg agtgggattt acggtcacag
     4141 tagttgtgta ttgcgatggt cccatcaaat cggtaggaaa agaaacatct cctacattgt
     4201 tcgcctcgtt gtaatataca ttcaggtagt aattaccggg tgtggttttg atggagtcgc
     4261 ccatctgcag agtgatggtt tcacctgccg gaatggtcgt aatcgaatga attacatcct
     4321 gataaatttc atcatcgtcg gttttggtaa ttcggactcc aatctgtgca tagtaatctt
     4381 tggctccgtt gtttttaatt ttcatttcaa atcttccgtt tttgttttca tacaggttta
     4441 acaacaccgt aggtgcggcg acaaggctta attccggctt tgagttatct gaaatgaaat
     4501 cgatagtggt actggttatt tttgcataca ggtattttct gcccgaatta tttaccagta
     4561 ccggaacaaa caggttatgt tcgtttctgt aaaatgctga aatttcataa tctccgtttg
     4621 gtattgaggg cttgaaatta acatcgtaat tcaatgcgga gtaataataa tttggaggca
     4681 ggttattact ccactcttta tctaccaaca gcgtatcaat cgtcactccg ttctgcgaaa
     4741 gtatcagcgc tgttcccatg atgctgctca gatagtcaaa agctcctaca ttcattaact
     4801 tctttatact caaagttacg cttcccgttc tgggaattgt tctttgcgaa ggacttatac
     4861 tgtcaatggc aacaagggaa gcgtgaaccg agcccgacac cggtttttga attccggtaa
     4921 ctatataatg acctaaatta tagccatcgt ttccggagcc gattcccgga ccgtcgggat
     4981 tcattgctga aatctcgaaa taaccgttgg atgagccgcc ccagccccag tttaaatgaa
     5041 acaatccatt ggcatcataa ccatcacata caaaaatgtg cccggcatta tcggctgtgc
     5101 ttccgcggta gagtacaggc cttcgggcat cgagttcggt aatcagaatg ttttcccagg
     5161 cttcgtactg taaatattta cgctgataaa tctgaattcc ggcgtcgtat ccgaaattat
     5221 tgtaaagagc cggcggaata tcggtggtgt aggaactgct gcctccattt gcagaagtgt
     5281 tgtaatccat tttgatggaa atacccacgt gtagcatcaa tttggctatg gcagcttttt
     5341 gcaggctgtt ttcagttccg gaagaactgt aatatgggag agtatttgtc cagtcgtagg
     5401 tggtatttcc aaagtcggca gataaattaa aatggttggt agccgatgta tatgctttta
     5461 tgccggtacc tttcacaggc cagttgtagt atttcattac ctgagccaca gctgttgctc
     5521 cgcatccggt tactgttctt acgtcggcca gcgccgggca cgaatcgttg tagggcttac
     5581 cctgatcgta tgagatgttt ccgagcagag gctccacggt cagattttga cgtgcagttt
     5641 tccggatagt ccggagcggg ctgcttgttt ccgaatccac atccggaaga ttaccgatag
     5701 caaacctgat ttgattggca tactcattca gccacgcttt caggttcacc ggaatccgca
     5761 agatgtcaaa tgaaccggtt aaggagtaac ccagtacttt tttggtgcgc gaatcggccg
     5821 aaacgattac aaatccgttg ttctgaccaa tattaaatac ataataataa ctggattgtg
     5881 gtaaattctt aacatcggca atatcggtac acgtatacgc cagttcgaaa ttcatggcgg
     5941 atttcatgcg caaagaggaa gaaattcccg atgaaaagta ctttttcgca atgttttgtg
     6001 cctcttcttt atctatcgga cgagcaaata aagtgattga aaacagaata gtaaaaagga
     6061 aaacggaggt ttttttaagt gtaaacatag tcgtaatttt tcgatgaaaa cgttgcagaa
     6121 tcaaatgaaa taaacgaaaa gaaaggaagc gcaatattat tcattaataa ttgattccgc
     6181 aacatttttt tacatttttt tatacgaata ttgtattata ccaggagaag ccttctgtag
     6241 ccctaaaaca ccacaggaaa ggtattatac gaaatcagca accggagaaa aaccaaagat
     6301 aaaataacaa aggcccgaag ggtaaataaa tgaattgcaa ttacagacat cacctgaatt
     6361 tcattaccac aaataaaaaa agttaaaccg atgtcaatga ttcaatataa atcaccactt
     6421 ttattaacaa ccgaacaaaa aattattaca tttgcatccg ggtttttctt gtttttgtgt
     6481 aacaatacac tattcatcca atgatttatt tcaaaacgtt tgataatctg aatgatgttt
     6541 tacaaaatgc aatttactaa attatcagga atttacaggt tttttgttac tgtatttctc
     6601 atggcaggaa ttgtcatcag tactgattca cagaccatta atttttggtt cacaaacgga
     6661 gattcgcaaa actatcagct tacgtctgca tcaaaaattc aatttctgaa tggcagcttg
     6721 atattcaaca acataagcac cactcctatt caacaaatcc gtaaaatcac ctttaatacc
     6781 cctacagaag ccaaagaaat tacagcactt caaccccacc ctgttttgag tccgaatcct
     6841 gccaaagatt atgtgatatt cagcaattta ccagctaagg aagaaatcgt ttcggtatac
     6901 agtatcacag gggcaattat actcacatca cgaataagtt cttcttcaaa cattctggat
     6961 atcagcatgt tgaataaagg tgcatatgta gtgaaaacgc aaaacttcag catccgtctc
     7021 attaaactat gaagaaaaca attatcatac ttaccgccct gatattttcg gttatttcat
     7081 tggtggctgc cgattatctt tttattcacc tgaaaaataa taccgtcgtt gccttggata
     7141 tgacttccct ggacagcatc gctttcactt ccggacagtc agaactggct gtttacagaa
     7201 atacacaatc tgcaaacgta tatccgatag ctgcaatcga cagcattaca ttcgcttcca
     7261 tgaccgacga gaccattcgg atcatttact acggcacaaa cgtttccatc atcaatccat
     7321 ttacctgcag aggtgttgat gtggctgttt caggtgctga tgtcactatt aattcgaatt
     7381 tatccgataa ggaaatcacc tgcagcgtat ccggaaccac taccgacggc tcgctgaaaa
     7441 tatacagcaa cttcaggcac accctggcac tgaacggcgc tgacattact aacacacgcg
     7501 gacctgccat caatattcaa tccagaaaaa aatgtacctt tcttctgacc gacggcacta
     7561 ccaactcgct tgctgacgga attacctatg cttcagatcc cgaagaccag aaatctaccc
     7621 ttttttccga aggtcaactc attataaaag gaggcggtac gctcaacatt ttgagccggt
     7681 caaaacacgc aatctgcagc gatgattaca tcgaaattga agggggcact atccacgtaa
     7741 cttcagcggc aaaagacggt tttcatgcca acgatcactt caatctcgaa gccggcacgg
     7801 taaccgtagc agccactggc gatgctgtgg attgcgaaga gggaaatata aaggtttcgg
     7861 gtggttccat cacatcaacc attgctacga acgacaccaa aggacttaaa tccgacagta
     7921 caatattcat ttcaggagga attattgact tcaccgtatc cggaaaacag gcaaaaggac
     7981 tgaaatcagg cggaaacatc acaattacag gaggtataac aaacattacc tgttcgggtg
     8041 gagttgttct tcaggcatcg ggttcaggat ccgatccatc atattgcaca gccataaaaa
     8101 gcgataaaaa catactcatt tcgggcggcg atgtcaatat caatcattcg ggagaagccg
     8161 gaaaaggaat ttccgccgac ggaaacctgg aaataagcgg aggcagaatt acttcaaata
     8221 cttccggcaa cggaaatact tacaccaact catccggatt aatggatggg tacaacgcaa
     8281 cctgcataac cactaattca gacttacgcc tgattgcggg agacatcacc tgctcagctt
     8341 cgggaacagg aggcaaggga attagtgcag ggggaaatat cattgttggc agtagcgagg
     8401 gaaatcctgt cgtttcactc actaccacag gtaaggaaat ttcggtaact tcgggacgta
     8461 caatcacgac gtatgcctca cccaaaacta tgaaagctga cggtgacatc acgttcaata
     8521 acggcactac aaccatctgg tcatcggacg acgggataaa atcggaatca aacgtgtatc
     8581 tttacggagg aaatattaat gtaaataaat catacgaagg cattgaagcg ccatacattt
     8641 accttagcgg agcatatgca gatgtattgg ccagtaacga cgccatcaac acaactaaag
     8701 gaacaagaag cggaggtgtg gaaacacccg atggaagttg cttatacatt aactcaggca
     8761 cactgatagc cacctgtacc aacggtgatg gcattgacag taacggcagt ataataatga
     8821 ccggaggaac cgcaatagtc aacggtcctt ctacaggagt ggaagaagct gtggactaca
     8881 atgacaattt caacatgaac ggaggggttt tcatcggagc cggttcgaac tccagcatga
     8941 caaaatcgat gagcgtaacc tccacacaac cgaacatgta tgtttcatcc ggttcaatga
     9001 tttcatcagc tacgttgatt gatatcagaa taaattctac cgacgtgatt accttcaaac
     9061 cgaaatatgg tgcctacaaa ttcctgttat ccactccgaa aatgaccaaa gaagcatcat
     9121 acaccatcta cacaggaggt tcgtattcat ctgcaactaa caccgcggga ctttacaccg
     9181 gaggaactta tactccggga acatcaaaaa aatcaggtac tttaagttct tcgggaacag
     9241 tcacttccgt atcattctga aacccgtgat ttattccgga tgacatgctt acaggtcagt
     9301 tgtttttaaa agaataaaaa aaaccatttg aatcactcca aatggttttt ttattcctga
     9361 tttcacaaaa tcagggttta acgggaaatt caatctgcaa tccgtaaagg ttgctaacat
     9421 tttttgcatc agactcaagc agaaagttga gtttcacctt atcgctggtt ttttttgcca
     9481 caaatttaaa cggaatgttc atccatacga ttttcggtgt aaagtaaaga gttcctttta
     9541 caaagtcgga gcgcgattcg aagaaagttt tgatagaatc gggaatgatc acttcaatat
     9601 ctcccggatt ggtcacttca tattcgaatg cggtgaggtt attcgtaaaa ccctcaccga
     9661 taacactgat tgtcactgta tcatttacat gcagggaatc aaacttgata ttgccctcgt
     9721 tcgtatagct catccgaagg gtactgtcgg tattgatcag agcagggttc atcagtgtaa
     9781 ggcggggaga ataattggag tcgttgccca gtttgcagct gttcatggca acaacgagtg
     9841 ctatcagtaa aaccggaaaa aacaatttac gtttcataat gcattttctt ttatttggaa
     9901 aaaaatcacc cgaacagcat caaaaaccgg gccgtaagag gttcatttta gttaaaattg
     9961 atttaaataa ataaaaaaaa agagaaccaa cggaatgatt ctcttttcag tcgggatgac
    10021 aagactcgaa cctgcgacct ctacgtcccg aacgtagcgc gctaccaact gcgccacatc
    10081 ccgttcacat tttgtgggtg caaatttact gcttttttgc tgaaaacaaa acagtaaagg
    10141 caatttcttt cagataaatt atactttgtg ctataagcat cagacataca actgtctgaa
    10201 aaaagatttt ttgtatcttt gcaaaaacaa aaaaaatgat gattgaaaaa gccattgaca
    10261 attacctgag caaaaatcat ttcagtgaac tcagtctgaa agctgttttc ttcgatatgg
    10321 acggcgtgct gttcgactcc atgccccacc actccgtagc ctgggtcaaa gccatgaacg
    10381 aatctgggct ggaattttcg gagcaagagg cttatatgaa cgaaggaaag acgggcactg
    10441 caaccatcaa cggcgtattc gtgcgcctca atggccgcga agccaccgaa gccgagaaac
    10501 aagacattta ccgactcaaa gcgcagtatt tcgaagagct cggagctata ggcacaatgc
    10561 cgtatgcact ggaaatgctg aagaaaatca aggaaaaagg ataccgcatc ttcgtagtca
    10621 cgggctcggc tcagcccacc ctgctcgaca gtctgaagga tcattttccc ggcatttttg
    10681 aaaaagagaa cattatttcg gcttttgacg taacgcaagg caaacctcac cccgagccgt
    10741 acctcaaagc actgcagaaa gcgggcgtca aaccctggga agccgtggtg atagagaatg
    10801 ctccgatggg cgtacaggca gctgtagccg cgcgtatttt cactatcggg gtcaatacag
    10861 gcccgcttga cagtaatgtg ctgactgaaa acggtgccga tctggtactc catagtatgc
    10921 aggaactcta cgaaaaatgg agtgatttcg gattctgaaa aaaacgctga aactctatgt
    10981 ttatcaaacg tataatcacg gctatatcac ttctgatgat tgtagcaggc ctcacagcct
    11041 ccgaaacgga ttcgctgaaa aagtacctgc cacgcattga aggaacaata cgggcaaaat
    11101 atgaatacaa caccagcctg gatgcttccc gctttcagat ccggagtgcc cgcttcagca
    11161 tcaacggcag tttgagcccc gttacttcct acaaagctga aattgatctt tcggacgagg
    11221 gagaaaccaa aatgctcgac gcctatgttc ggctgaaacc tgtggattgg ttcaatttta
    11281 ctatcggaca gcaaaaaatt cccttcggaa cggataatct gcgttctcct catcagcttt
    11341 actttgccaa ccgttcgttt gtgggtaaac agttggtcag cggactgcga gatgtgggag
    11401 ctacactgat gttttcgaac cggaaagccc ttccttttga ttttcaggcc ggagtgtaca
    11461 acggcgacgg actctacaac cagcaagact ggcagaaatc gatgaattac gcattcagga
    11521 tggagatttt cccgacagaa tatatggaaa tcagtctgaa ctacaattcg gtcatgccgg
    11581 ataatctccg gatgaacctg tacgacgtag gagcatacgc caatttctcc aacctgcatc
    11641 tggagggaga atacatttat aaaacctatc agggtgaggt ttttccggct accaaatcat
    11701 attctgtatt tgccgtttac gatatatgga ctaacaaacg ctttctgaaa aaaatcacac
    11761 cgcttgtccg tttcgactcc atgaccgata acaacaaggg gaagccctcc gaaggcaatg
    11821 tatatcaaac agacgatgtg gcaagcaaac gtctgaccgg cggcatcacc ttcagcctgg
    11881 gcaaaccgtt tcagaatgat atacggctca actacgaaaa atattactat gctgcaaatc
    11941 atgtgaacag cgatgataaa ctggtgctcg aatttgtagt tcgtttctaa ttcattttta
    12001 aaattaactg tatgcaactt atcaaatatc ccgccagaga acagtgggca gagctactca
    12061 aaaggccatc gctggatgta gcatcgctct atgaccaggt taaaatgacg ctcgacgaag
    12121 tccgggagca gggcgatacc gcgctgcgaa atttcaccct gaaattcgac aaagtggacg
    12181 tgaaagtacc tgaagttacg aaagaagaaa tagctgaagg ggtaaaagct gtttcggtag
    12241 aactgaaaca agccatcgaa atggcacgcc gaaacatctg gaaattccac tccgagcagc
    12301 agcgcgaata taccgagata cagacctcgc caggcgttta ctgctggcag aaagcagttc
    12361 ccatcgaaaa agtaggtctt tatgtaccgg gtggcagtgc gccgttgttt tctactgtgc
    12421 tcatgctggg catacccgct cagatagctg aatgcaaaga agttatcctc tgtacacctc
    12481 cgggtatgga tggaaaaatt catcctgccg tactttatgc cgctcaggtg gccggtattc
    12541 accgcatctt caaggcaggg ggtgctcagg ctattgcagc catggcttac ggcaccgaaa
    12601 gcattccgaa agtgtataaa atcttcggtc cgggcaatca gtatgtcacg gcagccaagc
    12661 agctcgtttc gctcaccgat accgccatcg atatgccggc aggaccttcg gaagtggaaa
    12721 ttattgccga cgaaactgcc agtgcagcct atgtggcttc agacctgctc tcacaggcag
    12781 aacacggcgc cgacagtcag gccatactgg tcaccacgtc cgaaaatctg atggacgaag
    12841 tactccggca gatagaaata cagatagaaa aacttccgag aaaagaaaca gcccgcaaag
    12901 cgctcgaaaa cagcaaaata atccttgtaa aatcgctgga tgaagccatt gccatcacca
    12961 acgagtatgc accggagcat ttgattattt ctactcgcag ttatatggtt gtagctgaac
    13021 acattatcaa tgcgggttca gtctttttag gtaatatgag tccggaaagt gccggtgatt
    13081 atgtttccgg aacaaaccac acactgccta cacacggttt tgcgaaagcg tacagcggcg
    13141 taaatctgga cagttttctg aagaaaatta cctttcagca gctcactccc gacggactga
    13201 ccaacctcag caatgccata attctgatgg ctgaaaatga aggacttcag gcacatgcca
    13261 atgcagtgaa agtgaggata gaaaagcctg aactgtgatt attatgtttt tttttgatta
    13321 atctgatttt atggataaaa aaaccgatag taaaaattct gaagaacgcc tggaaaaagc
    13381 ccctttctgg gaattgaggg ggcttctccg caaaaatatc caatcgctgg agccttattc
    13441 ctgtgcgcgc gacgagttta agggagaagc atcggtgtat ctggatgcca acgaaagtcc
    13501 gtacaacgct ccctataacc gctacccaga tccgctgcaa tggaaactga aagaaaaaat
    13561 cagtgagat
//
LOCUS       DAORKP010000049        13530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_406_length_13530_cov_9.870649, whole genome shotgun sequence.
ACCESSION   DAORKP010000049 DAORKP010000000
VERSION     DAORKP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_406_length_13530_cov_9.870649"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLN75_04225"
     CDS             complement(<1..489)
                     /locus_tag="PLN75_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRG03357.1"
                     /translation="MTPLEIQHIRTDFPILTEKIYNKPLIYFDNAATTQKPKAVLDRI
                     MYGYEHLNANIHRGVHFLSQKATEAHEEARKFVASYINAASSDEIIFTRGTTEAINLV
                     AFSYGEYACSESDEIVISVMEHHSNIVPWQMLCERKGMKLRIIPINEKGELNPDVFRS
                     LIN"
     gene            complement(703..1635)
                     /locus_tag="PLN75_04230"
     CDS             complement(703..1635)
                     /locus_tag="PLN75_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRG03358.1"
                     /translation="MNTLNFSNGDSMPVLGLGTWLSKPTEVYEAVVEAVKTGYRHIDC
                     AYIYKNEKEIGKALKYLFAENIVKREEMFITSKLWNNFHSPEQVEPAIRQSLKDLQLD
                     YLDLYLIHWPLAFKKEHAQTSDDQYSLDEMPLSATWKAMEGIKEMGLAKHIGVSNFSI
                     PKLTSIIENSAVKPEVNQVEVHPYFQQDKLIAFCKENNILVTAYSPLGSRHMMQSEES
                     ITKNPVVQKIAEKHNCTEAQVILAWGMERNTAVIPKSVNTERIRENFGSLQVKLDESD
                     MSELAKLNRNLRNAKGLYAVVPHGPYTYKNLWDE"
     gene            1765..3669
                     /locus_tag="PLN75_04235"
     CDS             1765..3669
                     /locus_tag="PLN75_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRG03359.1"
                     /translation="MRKNILAFCLTLFFSGMYAQQAPATHLNIIPEPVSTIVKKGTFS
                     PPKHITVSRPDGADNDLTVNFLTEKLRNFTDGKVTVKNSGKATIQLILNTTKDTSIGN
                     EGYTLTVLPKNITIRANSAAGLFYGVQTLVQLFPPEIESPHTTACNDVKIPVLEITDY
                     PRVGWRGLMLDVARHFFTVDEVKQYIDKMVRYKYNMLHWHLTDDEGWRIEIKSLPKLT
                     SVGAWRVEKTGWFGSFAPAKSDEPKNYGGYYTQEQIKDVIAYAKERHVQIMPEIDVPG
                     HSSAALAAYPDLSCFPETDDKHVRDGAPFLDWNTGGRPAAIMENTLCPANENVYDFMD
                     KVLTEVAALFPFEYIHTGGDEAPYTFWEKSPAVKQLMEREGLKNMAEVQSWFGKRVEK
                     IILAKGKKMMGWDEILEGGITPTTGLMSWRGISYGIEASKSGHPVVMSPSNFVYIDLM
                     QGDVSTEPRVYNSLRLNQTYKFNPIPEGSDGRYILGGQGNLWTEQIYNMRQAEYMTWP
                     RGLAIAESLWSPEGKKDYNNFIRKTENHFIRFDFAETKYSRAMYDPIIRVKKEGENYL
                     VELTPEIEGLDIYTSFDNSEPDRFYPKYSSPQFIPKDAGQMKITTYKGTKRVGRMITL
                     QVEDLKRRVK"
     gene            complement(3735..4637)
                     /locus_tag="PLN75_04240"
     CDS             complement(3735..4637)
                     /locus_tag="PLN75_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRG03360.1"
                     /translation="MTNNTCPICGNDTFSKVFDCKDHFVSGETFEIVECEKCKMRITK
                     DFPAENEIGKYYETSDYISHSDTKKGIVNAVYHRVRKLMLRKKAGWIEENTGLRSGKL
                     LDVGTGTGYFAAEMKARGWDVSVVEKNDSARRFAEKNFQLTAFPSIDDFVKKHAPEHP
                     ETFDVITLWHVLEHIENLNDTANQLVTLLKTGGKLVIAVPNCNSYDALKYGNLWAAYD
                     VPRHLWHFRKEQIEMLAGIHGLYMTDVKKMPFDAFYISMMSEKYSRKKFPFIRGMMSG
                     SGGLFSSFSDVTKSSSLVYFLEKE"
     gene            4884..7388
                     /locus_tag="PLN75_04245"
     CDS             4884..7388
                     /locus_tag="PLN75_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRG03361.1"
                     /translation="MIFTKTNLRSLTPHLVALLLFIFIAFAYFLPVIEGKELVAHDTD
                     SWRSMAQETIEYNKSHDDVTLWTNSMFGGMPNYQISMVQPNNVLQYVEKVIMSFPRPV
                     SNVFLYLICFYILLLSFGLKPWQAIAGAIGFTFASYNFIVIAAGHNSKAITIAYVAPL
                     IGAVFLAFREKRFLGAVLTAFFLSLAIRANHIQILYYTLIILFFFAIVEFIFSIREKK
                     FPDFLKSAGVLIIAAIIAVGMNATSLLTTYEYSHYTMRGKSNGLTADTQSSQHGLNKE
                     YITQWSYGVDETLTLLIPDFMGGASTGKLSMKSETAGHLKNLGVPDGQIKQIVTQLPL
                     YRGTQPGTSGPVYLGAIVIFLFVLSFFVVDKKLKWWLVPMIILTMMLSWGKNFMPLTD
                     FFIDYVPMYNKFRAVSMTLFATGFGIALLAFLAMKEVYEGNISKEKLTKSITVSALIT
                     GGICLVFALVPSLAGSFVSPQDAQFQGDYAFLKDTLPPDRQSLLRSDAFRSLVFIILG
                     AFVLWSFVKGFLKKNVSIALLAFFILIDLFPVAKRYLNDDNFESKRPTQLVQKTPADE
                     AILQDKSQFRILDATVDIFNDATPSYFHKNIGGYHAAKLRRYQELINMQLTGEIGKLF
                     GAFGSATTAESITPVFDSLRVMNMLNMKYVIYNKQAPPLVNPYHNGNAWFVNNIRIAQ
                     DANEEMKLLGEIDTRHELVIDKSLASALPSKVTPDSTAKISLKSYAPNHLIYSVDTKT
                     DQVAVFSEIYYDKGWKATIDGKEVPYTRVNYLLRAMPLKAGSYDVEFRFAPGSYSTGN
                     LLALISSVLLIIGLATYLFLFFRKKKSGAAEMNTAK"
     gene            7417..8301
                     /locus_tag="PLN75_04250"
     CDS             7417..8301
                     /locus_tag="PLN75_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRG03362.1"
                     /translation="MPKKKIRKKKYYVRNAGVTATFSMSLVLFLIGLLALSLFLTRDM
                     TNFVKENLNLSVVLSEEITESQTKAIEKYLTKSDFAKSVEYISKEQALKEHVEYLGED
                     PEAFLGYNPLFAQFEVKLNARYANNDSVPVIESKLKRFGKSIEEVSYQKDVIAQVNEN
                     IRKISIILIGIVSVLLLISFALINNTVRLRIYSNRFLINTMKLVGAKSWFIRKPYMKQ
                     SVINGLVAALIALGLLAALVYYFKFNFGLDTDLIKQKTALIVAAIVIVSGVILTAISS
                     YFAVGRYIRMRTDDMFYV"
     gene            8384..8617
                     /locus_tag="PLN75_04255"
     CDS             8384..8617
                     /locus_tag="PLN75_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRG03363.1"
                     /translation="MEEKKFGLSKINYILIAAAFLVVVLGFIIMGGDPSGEVFNPDIY
                     STKRIIVGPMISLAGFIFMVVAILYKPKKKVQE"
     gene            8614..9411
                     /locus_tag="PLN75_04260"
     CDS             8614..9411
                     /locus_tag="PLN75_04260"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRG03364.1"
                     /translation="MSIFEAIVIAIVEGLTEFLPVSSTGHMILAQGFLGVKSDEFVKA
                     FTVIIQFGAIISVLVLYWKRFFQSVDFYLKLLIAFLPAAVIGFLAGDFIDSLLENVWV
                     VAIMLVLGGIFMLFVDKWFTHPQENEPEMTLKRALKIGFAQCVAMIPGVSRSMATIVG
                     GMAQGFSRKTAAEFSFFLAVPTMAAASGYKFLKLLMEDTTRSMLTENMNVLIVGNVVA
                     FVVAMLAIKFFISFLTRYGFKAFGYYRIVAGLAIILLLVLGYNLEVA"
     gene            9414..10127
                     /gene="truB"
                     /locus_tag="PLN75_04265"
     CDS             9414..10127
                     /gene="truB"
                     /locus_tag="PLN75_04265"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRG03365.1"
                     /translation="MDFTEGEILYVDKPLGWTSFDVVNKIRWQISRTLKIKKVKVGHA
                     GTLDPLATGVMIICTGKATKRIEEFQYQTKEYIATLELGATTPSFDLEHEVDERFPTG
                     HITKELIDKVLPSFKGEIWQVPPMYSAVKVEGKRAYKYAREGQEVELKPKLLVIDEIE
                     VLDFSLPVLKIRVVCSKGTYLRALARDIGEALQSGAHLTALQRTRIGDVRLENCRQMG
                     DLLNHISGISGNEKQVIPD"
     gene            10236..11285
                     /gene="queA"
                     /locus_tag="PLN75_04270"
     CDS             10236..11285
                     /gene="queA"
                     /locus_tag="PLN75_04270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRG03366.1"
                     /translation="MKLSQFKFKLPDELIAQYPAKFRDESRLMVLNKKTGEIEHKTFK
                     DILDYFDEKDLFVFNDTKVFPARLYGNKEKTGAQIEVFLLRELNREGRLWDVLVEPAR
                     KIRIGNKLYFGDDDTIVAEVIDNTTSRGRTLRFLYDGDHETFKTELYKLGETPLPRNI
                     TRPVEPEDEENFQTIFAKNIGAVSAPAAGLHFSRELMKRMEIKGIDVAFLTSHMSLGN
                     FREIDVEDLTKHKMDSEQMEITPETAETVNNAQDEARKICAVGVTVMRALESVVGTEG
                     KIKPFSGWTNKFIFPPYDFTVASAMITNFHLPYSTLLMLTAAFGDYDYVMHAYDVAVK
                     EKYQFGVYGDAMLII"
     gene            complement(11592..12128)
                     /gene="coaBC"
                     /locus_tag="PLN75_04275"
     CDS             complement(11592..12128)
                     /gene="coaBC"
                     /locus_tag="PLN75_04275"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRG03367.1"
                     /translation="MKTVLIGVTGGIACYKSIEIVNQLRKDGYNVEVIMTRSAQEFVT
                     PLTFQTMSGNQVVTDMFDPVHKWDTKHIELAKKAALFVIVPATANVIGKIANGIADDM
                     LTTTVMAARCPVLIFPAMNSYMYENPIVQDNIEKLKKYGYHVYKTASGELACGDTGSG
                     KLPDWQEIVKEIEKFCNK"
     gene            complement(12183..12956)
                     /locus_tag="PLN75_04280"
     CDS             complement(12183..12956)
                     /locus_tag="PLN75_04280"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRG03368.1"
                     /translation="MINIAITAGGTSENMDGVRKITNISTGQLGWHCLEAILDYMLQS
                     DTEDFHVHYLLTETAARKTLNENQQKFVSFIEVTDAESVFKAVDKLTKETKIDFFIHS
                     MAVSDFTFSYAANIYRLAEEIEALQCERGFTNDDVRFLLENPQTQHRKDEKISSDNEL
                     IIGLKPTKKVIPLIKQNNPATFLVGFKLLRNATEEKLMAAAEKLTAQNGCDMVFANEV
                     SLIGEKNHEGILIKGNKIIDRPRGKKQIAKSIVNNMLCH"
     gene            complement(13068..13451)
                     /locus_tag="PLN75_04285"
     CDS             complement(13068..13451)
                     /locus_tag="PLN75_04285"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRG03369.1"
                     /translation="MLLTLLKAKLHRATVTEANLNYTGSITIDKDIIDQAGIFIHEKV
                     QIVNNNNGARFETYVIEGKSGSGVLCLNGAAARLVQPGDKVIIMAYAQMTPEEAQTHK
                     PVVLLLGDNNQILEKLSERAGQIIE"
BASE COUNT         3768 a         2778 c         2964 g         4020 t
ORIGIN      
        1 atttatcaga gaacgaaaaa catccggatt cagttcccct ttttcgttta tcggaataat
       61 gcgcagtttc atccctttcc gctcgcaaag catttgccac ggaacgatgt tggaatgatg
      121 ctccatgacc gaaatcacaa tttcgtcgct ttcgctgcag gcatattcgc catacgaaaa
      181 tgcaaccaga tttatagctt cggtagtgcc tcgggtgaaa atgatttcat cagatgaagc
      241 agcattgatg tatgatgcaa cgaattttcg ggcttcttca tgggcttccg tagctttctg
      301 gctcagaaag tgtaccccgc gatgaatgtt ggcattcagg tgctcgtagc catacataat
      361 tcggtcgagc actgcttttg gcttttgggt ggtggcagcg ttatcaaaat agataagtgg
      421 cttgttgtat atcttctctg taagtattgg gaaatcggtt cggatatgtt gtatttccag
      481 gggagtcatt attttcaata caaatctgtt tattgactac aaaattagtg tttttttatg
      541 aattatatgc aatgaagatg aaaaacaact cacgctcaac cttgtacaga aggaatgttg
      601 aatttttcac aacgtaatcg attgacatat gaactgattt tttaacagaa ccgggagcac
      661 tgaaaatgat tttttcggtt attacagtgc taaatacgct cgttattcat cccagagatt
      721 tttataggtg taaggcccgt gaggtacgac tgcataaagt cccttggcgt ttcgcagatt
      781 ccggttcagt ttagccaatt ccgacatatc actttcatcc aatttcacct gcaacgagcc
      841 gaaattttcg cggattcgtt ccgtgtttac cgattttgga atgactgctg tgttacgctc
      901 catgccccag gcaagaatca cctgagcctc cgtgcagtta tgcttttcgg ctattttttg
      961 tacaaccgga tttttggtaa tcgattcttc ggattgcatc atatgccggc tgccaagcgg
     1021 cgaataggct gtgacaagta tgttgttttc tttgcagaaa gcaatcagtt tgtcctgctg
     1081 aaaatacgga tgaacctcca cctgatttac ttccggcttt acggcagagt tttcgatgat
     1141 ggaggtcaat ttcgggatgc tgaaattcga tactccgata tgcttcgcca gacccatttc
     1201 cttaatgcct tccatcgctt tccaggtcgc agaaagtggc atttcgtcca gcgaatattg
     1261 gtcatcggag gtttgagcat gttctttttt gaaagccagt ggccagtgaa tgagatataa
     1321 atccaggtaa tcgagttgca aatctttcag agattgacgg atggctggtt ccacctgttc
     1381 gggtgaatga aagttgttcc ataatttgga agttataaac atttcttcgc gcttaacaat
     1441 gttctcagcg aataaatatt tcaaagcctt gccgatttct ttttcatttt tgtaaatgta
     1501 tgcgcagtca atatgtcgat agccagtttt aactgcttcc acgacagctt catatacttc
     1561 cgttggtttt gagagccagg tgccgagccc caatacaggc atcgaatcgc cgtttgaaaa
     1621 atttaatgta ttcatcttat tcgttatttg tttttattgt ttgaacattg tggttgaaat
     1681 attttactgc gtaatttcag tttgcagaat actaacgaaa tttgtagctt tgtagttcaa
     1741 aaaaattacc ataaaatcac cactatgaga aaaaacatcc tcgcattttg tctgacctta
     1801 ttcttttcag gcatgtatgc tcagcaggca ccggccacgc acttaaatat tattcccgaa
     1861 ccggtttcca caattgtaaa aaaaggcaca ttctcgcctc cgaaacacat tacggtatcc
     1921 agacctgatg gtgcggataa cgacttgact gtcaatttcc tgacagaaaa actcaggaat
     1981 ttcaccgacg gcaaggtgac ggtaaaaaac tccggtaaag ctaccattca actcatcttg
     2041 aatacaacaa aagatacatc gattggtaat gaaggatata cactcactgt tttaccaaaa
     2101 aatatcacta tcagggcaaa ctcggctgcc ggcttgtttt acggagttca aacgctggta
     2161 caactttttc cgcctgaaat tgaaagcccg cataccactg catgcaatga cgtgaaaatt
     2221 ccggtgcttg aaatcaccga ttatccgcgt gtaggctggc gggggttgat gctcgatgtt
     2281 gcacgccatt tttttacagt ggatgaagtg aaacagtaca tcgacaaaat ggttcgctac
     2341 aaatacaaca tgcttcactg gcacctgacc gacgatgaag ggtggcgcat tgaaataaaa
     2401 tcactcccga aacttacatc ggtgggagcg tggcgggtgg aaaaaacggg ttggtttggc
     2461 agttttgctc ctgcaaaatc cgacgaaccc aaaaattacg gcggatatta cacgcaggaa
     2521 cagatcaaag acgtgattgc ttacgccaaa gaacgtcatg tgcagattat gcctgaaatt
     2581 gatgttccgg gtcatagttc ggctgcactg gctgcttatc cggatctgtc gtgcttcccc
     2641 gaaaccgacg ataaacacgt gagagacgga gctccctttt tagactggaa tacaggcggg
     2701 cgacctgcag ctatcatgga aaatacactt tgcccggcca acgaaaatgt gtacgatttt
     2761 atggataagg tgctcaccga agtggctgcg ttgttccctt tcgaatacat ccacaccggc
     2821 ggagacgagg ctccctacac attctgggaa aaaagtcccg ctgtgaaaca gctgatggaa
     2881 cgcgagggac ttaagaatat ggccgaagtt cagtcctggt ttggaaaaag agtggaaaaa
     2941 atcattcttg ccaaaggaaa aaaaatgatg ggttgggacg aaattctgga aggaggcatc
     3001 acaccaacca ccggactgat gagctggcgc ggaattagtt acggcatcga agcttcaaaa
     3061 tcgggacatc ctgtagtgat gagcccgtcc aatttcgttt acatcgattt gatgcagggc
     3121 gatgtgagca ccgaacctcg cgtttacaac tcgctaagac tcaatcaaac ctataagttc
     3181 aatccgattc ccgaaggttc cgatggccgg tatatcctgg gaggtcaggg aaatctgtgg
     3241 accgagcaaa tttacaacat gcggcaggca gaatatatga cctggccacg cggacttgcc
     3301 atagccgaat cgctttggtc gccggaaggc aaaaaagact ataataactt tatacgaaag
     3361 accgaaaacc atttcatccg gtttgacttt gccgaaacaa aatactcccg ggccatgtac
     3421 gatccgataa tacgggtgaa aaaggaagga gaaaattatt tggtggaact tacacccgaa
     3481 attgaggggc tggatattta taccagtttt gataattcgg aaccggatcg tttctatccg
     3541 aaatactcga gcccgcagtt cattcccaaa gatgccggac aaatgaaaat aaccacctac
     3601 aagggcacta agcgggtggg tcgaatgatt accctgcaag tggaagacct gaaaaggagg
     3661 gtgaaataac caaaaaacag cggctgcaca gctgctgttt tttggtttgt tcagcatgaa
     3721 aacagattga tttcttattc cttttcgaga aaatatacca gcgaactact tttggtaaca
     3781 tccgaaaaag acgaaaataa tccgcctgat ccgctcatca tacctcttat gaaagggaat
     3841 tttttgcggg aatacttctc gctcatcatg gagatgtaaa aggcatcaaa tggcattttt
     3901 ttaacatcgg tcatgtacag tccgtgtatt ccggcaagca tttctatttg ttcttttctg
     3961 aaatgccaca ggtggcgggg cacgtcgtat gcagcccaca gattgccgta tttcagggca
     4021 tcgtaggagt tgcagttggg tacggcaatc accagttttc cacccgtttt gagtagcgta
     4081 accaactgat ttgcagtgtc attgaggttt tctatgtgct caagtacatg ccagagagta
     4141 atgacatcga acgtttccgg gtgttccggt gcatgttttt taacgaagtc atctatggaa
     4201 ggaaatgctg taagctgaaa atttttttcg gcaaaccgac gtgccgaatc gtttttttcc
     4261 actacagata catcccatcc gcgtgctttc atttcagctg caaaatagcc tgtgccagta
     4321 cctacatcca gcaattttcc tgatctcaat cctgtatttt cttctatcca gcctgctttt
     4381 tttcgcagca tcagctttct aactcggtga tacacggcat tgacaattcc tttcttcgta
     4441 tcgctgtgtg aaatgtaatc ggaggtttcg tagtatttgc caatctcgtt ttcggcagga
     4501 aagtctttcg taatcctcat tttacatttt tcgcattcaa ctatttcaaa tgtttcgccg
     4561 cttacaaaat gatctttgca gtcgaaaacc ttgctgaatg tatcgtttcc acagatcgga
     4621 caggtattat ttgtcatttt tttaagttga ttattcataa agtgcaaaga tagtgcgttt
     4681 tcctgaaccg gaatccgaga agaagtattt tttatttaat tccgctttca tattttgaaa
     4741 aaacactttc tccggacatc gtatcatttt gcggtttttt ctcttaatta attaaaattt
     4801 acctctttaa tctgaacaaa accggtaaaa atgtactact tttgtatgat ttttcccaaa
     4861 aacttccatt ttaaaactga cgaatgattt ttaccaaaac aaacttgcgc tccttaactc
     4921 cgcacctggt tgcactttta ttatttattt tcattgcgtt tgcttatttt ttacctgtaa
     4981 tagagggtaa ggagctggtg gctcatgata cagatagctg gcggagtatg gcgcaggaaa
     5041 ccattgagta caataaatct cacgatgatg tgactctctg gactaattcg atgtttggag
     5101 gaatgcccaa ctatcagatt tcgatggtgc agcccaacaa tgtgctacag tatgtcgaaa
     5161 aagtgattat gagttttccg cgtccggtat caaacgtgtt tctttatctc atttgtttct
     5221 atatactttt gctgtcattt ggattaaaac cgtggcaggc aattgccggt gccatcggtt
     5281 tcacgtttgc atcttacaac tttattgtca tagctgcggg acacaattcc aaagcaatta
     5341 cgattgcata cgtagcacct ctcattggag ccgtatttct ggctttcagg gaaaaacgat
     5401 ttctgggcgc agtgcttaca gctttttttc taagtctggc tatacgtgcc aatcatattc
     5461 agattcttta ctacacgctc attatactgt tcttctttgc tattgttgag ttcattttca
     5521 gcatcaggga gaaaaaattt ccggattttc tcaaatcggc aggcgttctg atcattgcag
     5581 ctataattgc agtagggatg aatgccacct cgctgcttac gacctatgaa tacagtcatt
     5641 acaccatgcg cggcaaatcc aatggcctca cagctgacac tcaaagttcg cagcacggac
     5701 ttaacaaaga atatattact cagtggagtt atggtgtgga tgaaactctc acactgctta
     5761 ttcctgattt tatgggtggt gcaagcacag gcaaactcag tatgaaaagt gaaacggccg
     5821 ggcatctgaa aaatctgggg gttccggatg gacaaattaa acaaatagtt acacagctgc
     5881 ccctttaccg gggtacacag cccggtactt cgggtccggt ttatctggga gctatcgtga
     5941 tttttctttt cgtgctgagt ttttttgtag tggataaaaa gttgaaatgg tggctcgtgc
     6001 cgatgataat tcttaccatg atgctttcct ggggaaaaaa tttcatgcct ctgaccgatt
     6061 tctttatcga ttatgtaccc atgtacaata agttcagagc tgtgtccatg acactctttg
     6121 caaccggatt cggtattgca ctgctggcat ttctggctat gaaagaagta tatgagggta
     6181 atatttcaaa agaaaaactt acaaaatcga ttactgtcag tgccctgata actggtggaa
     6241 tttgtcttgt ttttgctttg gttccatcac ttgcgggcag ttttgtgtcg ccccaggatg
     6301 cgcagtttca gggtgattat gcgtttctga aagatacatt gccacccgac cgccagtcgc
     6361 tgctccgttc ggatgctttc cgttcgctgg tattcattat cctgggagca tttgtacttt
     6421 ggtcgtttgt taagggtttt ctgaaaaaaa acgtatcgat agcattgctg gcatttttta
     6481 ttctcattga ccttttcccg gtagccaaac gttacctcaa cgacgataat ttcgaatcaa
     6541 aacgtccgac gcagctggta caaaaaactc ctgccgatga agccattttg caggataaat
     6601 cgcagttccg catccttgat gctacggttg atatttttaa cgatgccaca ccttcctatt
     6661 tccacaaaaa cattggcgga tatcatgccg caaagctccg gcgctaccag gaattgatca
     6721 acatgcaact taccggtgaa ataggaaaac ttttcggtgc tttcgggtct gccactacgg
     6781 ctgaatcaat cactccggtg tttgattcgc tccgggtgat gaatatgctg aatatgaaat
     6841 atgtgatata caacaagcag gcaccgccgc tcgtaaatcc ttaccacaac ggcaatgcat
     6901 ggtttgtaaa taatatacgc attgctcagg atgccaatga agaaatgaaa cttctgggtg
     6961 aaattgacac ccggcacgaa ctggttatcg ataaatcgct ggcctctgca ctgccttcaa
     7021 aggttacgcc ggattcgacg gcaaaaattt cactcaaatc ttacgctccc aatcatctga
     7081 tttacagtgt ggatactaaa actgatcagg tggctgtatt ctctgaaata tattatgata
     7141 aaggctggaa agctaccatc gatggtaaag aagtacctta tacccgtgta aattacctcc
     7201 tgcgcgccat gccgctcaaa gccggaagct atgatgtgga gttccggttt gcacccggtt
     7261 cgtacagtac aggtaatttg ctggctttga tttcgtcggt gctgctcata atcggcctgg
     7321 caacgtattt gtttttattt ttcagaaaaa agaaaagtgg tgctgcagaa atgaatactg
     7381 caaaataatt tattccgatc aatcgaattt ttactcatgc caaaaaagaa aataagaaag
     7441 aaaaaatatt acgtcagaaa tgccggagtc actgccacat tcagtatgtc gctggtgctg
     7501 tttctgattg gtttgctcgc tctgtcgctt ttcctcacac gtgatatgac caattttgtt
     7561 aaagaaaacc ttaatctttc cgttgttctg agcgaggaaa taaccgaatc gcagacaaaa
     7621 gcaattgaaa aatacctgac taagtcggat tttgcaaaat cggttgaata tatttccaag
     7681 gagcaggcac tcaaagagca cgttgaatac ctgggtgaag atccggaggc ttttctggga
     7741 tataatccgc tatttgcaca gtttgaagtg aaactgaacg cccggtatgc caacaacgac
     7801 agtgtgccgg tcatcgaatc gaaattgaaa cgatttggta agagcattga agaagtaagt
     7861 tatcagaaag atgtgatagc tcaggtgaat gaaaatattc gaaaaatcag catcatactg
     7921 attgggatag taagcgtgct gttactgatt tcttttgcgt tgattaacaa tacggtacgg
     7981 cttcggatat actccaaccg ttttctgata aacaccatga agctggtcgg tgccaaaagc
     8041 tggtttatcc gcaagcctta tatgaaacaa agtgtaatta acgggcttgt ggcagctctc
     8101 atagcgttgg gtttgcttgc agccttggtt tattatttta aattcaactt tggacttgac
     8161 acggatttaa taaaacagaa aaccgcactg atagtagcgg caatcgtcat cgtttcggga
     8221 gtgatactta cggccatttc atcttacttt gctgtagggc gttatatccg aatgcgtacc
     8281 gacgatatgt tttatgtgtg atttcggaat tcagaataca gaatacagaa cacagatttt
     8341 gaagtattta caaatagaaa aaacgaaaaa aaactgacta aatatggaag aaaagaaatt
     8401 cggactttca aaaataaatt acatcctcat agcagctgca tttctggttg ttgtgctggg
     8461 ttttataatc atgggcgggg atccctcggg cgaggtgttt aatccggata tttacagcac
     8521 aaaacggatc attgttggtc cgatgatttc gttggcaggc tttatcttta tggtggttgc
     8581 catactgtac aaaccgaaaa agaaagttca ggaatgagta tttttgaggc aattgttatt
     8641 gccattgtag agggactgac cgaatttctg cctgtgtcgt ccaccggaca catgattctg
     8701 gcacagggat ttctgggggt gaagagcgat gaatttgtga aggcatttac ggttattatt
     8761 cagtttggag ctatcatttc ggtgctggta ttgtactgga aacgtttttt tcagtcggtg
     8821 gatttttatc tgaaattgct gattgctttt cttccggctg ctgttatcgg atttttagcc
     8881 ggcgatttca tcgatagttt gctcgaaaat gtatgggtgg tggccatcat gctggtgctt
     8941 ggcggtatat ttatgctctt tgtggataaa tggttcaccc atccgcagga aaacgagccc
     9001 gaaatgacat tgaaaagagc acttaagatt ggctttgcac aatgtgtagc gatgattccg
     9061 ggcgtttcgc ggtcaatggc taccatcgtg ggaggtatgg cgcagggatt cagccgcaag
     9121 acggcagccg agttttcttt ctttctggct gtaccgacca tggcagcggc ttcgggttat
     9181 aaatttctga aattattgat ggaagatact acccgcagta tgttgaccga gaatatgaat
     9241 gtgctgatcg taggaaacgt ggttgcattt gtagtggcga tgcttgccat caagtttttt
     9301 atcagttttc tgaccagata cggctttaaa gccttcggat attatcgcat tgtggcagga
     9361 ctggctatca tattactgct cgtgttgggt tataatttag aagttgcata aaagtggatt
     9421 ttacagaagg agagatttta tacgttgata agccgcttgg atggacctca ttcgatgtgg
     9481 tgaataaaat ccgctggcaa atatctcgta cactgaaaat aaaaaaagta aaggtaggtc
     9541 atgcgggcac cctggatccg ctggcaaccg gagtgatgat tatttgtacc ggaaaagcga
     9601 ccaaaaggat tgaagaattt cagtatcaga ccaaagaata catagcaaca cttgagctgg
     9661 gagccaccac accatccttt gatctcgaac atgaagtaga tgaacgattt ccgaccggac
     9721 atattaccaa agagttgatt gataaggttt taccttcgtt caagggcgaa atctggcagg
     9781 ttccgccaat gtattcagct gtaaaagtgg aaggtaaaag agcgtataaa tatgcacgtg
     9841 aagggcagga ggttgaactt aaacctaagt tgttggttat agatgaaata gaggtgcttg
     9901 atttttcgtt acccgtgctg aaaatcaggg ttgtgtgcag caaaggcact tatttacgtg
     9961 cacttgccag agatattggt gaggcattgc aaagcggagc acacttaact gcactacagc
    10021 gtacccgcat tggcgatgta cgcttagaaa attgtcggca gatgggcgat ttgctgaatc
    10081 acatctccgg aatatccgga aatgaaaagc aagtcattcc ggattgaccg aaaaacttac
    10141 aagaacaaat gatttaactt tttttgataa taagaaagaa atatgtatta tgtgattctt
    10201 tttgaattca aaatcaatag aaacttacag aaaaaatgaa attatcacaa ttcaaattta
    10261 aattgccgga tgagttaatt gctcagtacc cagcaaaatt cagggatgaa tcccgcctga
    10321 tggtattgaa caaaaaaacc ggagaaattg aacacaaaac attcaaagac atactcgatt
    10381 attttgatga aaaagatttg ttcgtgttca acgataccaa ggttttcccg gcacgacttt
    10441 atgggaataa agaaaaaaca ggtgcgcaaa ttgaagtttt tttattgcgt gaacttaatc
    10501 gcgagggacg tctgtgggat gtgctggtgg agcccgcgcg taaaatccgc attggcaata
    10561 agctatattt cggcgatgac gatacaattg tagctgaagt gattgacaac acaacttcac
    10621 gcggacgtac actgcgtttt ctgtacgacg gcgatcacga aaccttcaag accgaactgt
    10681 ataaactggg cgaaactccg ctaccgagga atattacacg tccggtagaa cccgaagatg
    10741 aagaaaattt tcaaaccata tttgccaaaa atataggagc agtttcggct ccggcagcgg
    10801 gattgcattt cagccgcgaa ctgatgaaac gaatggaaat caagggaata gatgtggctt
    10861 tcctgacttc gcacatgagc ctgggtaact tccgcgaaat tgacgtggag gatcttacca
    10921 aacacaagat ggactccgag caaatggaaa ttacacccga aacggctgaa acagttaaca
    10981 atgcacagga tgaagcaagg aaaatatgtg ccgtcggggt taccgtaatg cgtgcactgg
    11041 aatctgtggt aggcaccgaa gggaaaatca aacccttcag cggctggaca aacaaattca
    11101 tatttccacc ctatgatttc acagttgcaa gtgccatgat tactaacttt catctgcctt
    11161 actccacgct gcttatgctt actgcagcgt ttggagatta cgactatgta atgcatgcgt
    11221 atgacgtggc agtgaaggag aaatatcagt ttggagtata tggcgatgcc atgctgataa
    11281 tctaacatgc tataagtata accggaagcg ggaagaacga agatttatgc ttcgaacttc
    11341 ccgcttcttg ttttttagga aaaatttcaa aaaatcagac ttataggtca aaaagtaggc
    11401 taaaaactcc tgaattgcct gatatcatta tctttgaggc aagtcaaaga ttatgaacaa
    11461 taaaaatgga cttaatctga acattctaat tgcgtataaa ctttgataaa tgctcaaaag
    11521 ctcctccttt agagcctgtc ccagccagtc ccgattttat atctggaaac atagtacggg
    11581 aagagtgggg ttcatttgtt acaaaatttc tcaatctctt tcacaatttc ctgccagtca
    11641 ggcaacttgc cagagcctgt gtctccgcag gcaagctctc ctgaagccgt tttgtaaaca
    11701 tgatatccgt atttttttaa tttttcaata ttgtcctgta caattggatt ttcgtacata
    11761 taggagttca tggccggaaa aatcaatacc gggcagcgag cagccatcac cgttgttgta
    11821 agcatatcgt cggcaatgcc gttggctatt tttccgatta cgttggcggt ggcaggcaca
    11881 attacaaata aagctgcttt tttggcgagt tcaatgtgct ttgtatccca cttatgcacg
    11941 ggatcaaaca tatccgtaac cacctgattg cccgacatgg tctggaatgt aagcggagtc
    12001 acaaattcct gagccgagcg cgtcatgatc acttccacat tgtatccgtc tttccggagc
    12061 tgattgacta tctcgatgga tttatagcag gctattccgc ccgttacgcc gataagtacc
    12121 gttttcattg tgttttattt tgttgattga tttcgtgtgg atgatttata aatatgtgat
    12181 aatcaatgac acagcatatt gttgacaatc gattttgcaa tctgtttttt tcctctcggc
    12241 ctgtcgatga ttttattacc ttttatcaaa ataccctcgt ggtttttttc tccaatgagc
    12301 gatacttcgt tggcaaacac catatcacag ccgttttgag ccgtaagttt ttctgcagct
    12361 gccataagct tttcttcggt agcgttacgc agtaacttga atcctaccaa aaaagtagcc
    12421 ggattgtttt gcttaataag cggtatgacc tttttggtgg gcttcagccc gataatcaac
    12481 tcgttatcag atgatatttt ctcgtctttt ctatgttgag tttgtggatt ttccaaaaga
    12541 aaccggacat catcattggt aaaaccacgt tcacactgca atgcttcaat ttcttcagct
    12601 aagcgataaa tattcgcagc atacgaaaat gtgaagtccg aaacagccat cgagtgaata
    12661 aagaaatcga ttttagtttc ctttgtgagc ttatccactg ctttgaatac actctcagca
    12721 tccgtcacct caataaaact cacgaacttt tgttggttct cattcaaggt tttacgggca
    12781 gccgtttcgg tcagcaggta atggacatga aaatcctccg tatcgctttg cagcatataa
    12841 tccaatatgg cttccaggca atgccagccc agctgtccgg tgctgatgtt ggttattttc
    12901 cggactccgt ccatgttttc tgaagttcct ccggctgtaa tggcaatgtt gatcataaaa
    12961 ggtctcctaa ccccgttcgg ctgagctcac gtcgaagcct gaagggggaa taattgtgag
    13021 aatttgaaat gaaataatgg ttttgatgat aatcctttca aataatttta ttcaattatt
    13081 tgacctgctc tttctgaaag tttttccaga atctggttgt tatcacccag taaaagtact
    13141 accggtttgt gtgtttgtgc ttcttcgggt gtcatttgcg cataagccat gatgatgact
    13201 ttatcgccgg gttgcaccag gcgggctgca gcgccattca gacacaaaac accacttccg
    13261 ctcttacctt ctatcacgta ggtttcgaaa cgtgcaccgt tgttgttatt gacaatttgc
    13321 actttttcgt gaatgaaaat tccggcctga tctattatat ccttgtcgat agtgatgctt
    13381 ccggtgtaat tcaaatttgc ttcagtaacc gtagctctgt gcagtttggc ttttaataat
    13441 gtaagtaaca taagattatt tacgatttac gatttaacaa tttacgattg aaatctttcc
    13501 ctttaatcaa ctattcaact ataacccaga
//
LOCUS       DAORKP010000050        13456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_411_length_13456_cov_9.992165, whole genome shotgun sequence.
ACCESSION   DAORKP010000050 DAORKP010000000
VERSION     DAORKP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_13456_cov_9.992165"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PLN75_04290"
     CDS             complement(<1..1686)
                     /locus_tag="PLN75_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRG03370.1"
                     /translation="MKKTAIFLFLIFMLPVLAVGQVSFGKPEKINENWRFILQDVKNG
                     EEKNFNDSRWEKIDLPHDWSVKGLLSPTLASCTGYLPGGIGWYRKTLNIPKERENEKV
                     YLYFEGVYNRSEVFVNGHSIGKRPNGYISFLYDATPYVVFGEDNVIAVRVDHSRSADS
                     RWYTGSGIYRNVWAVYSNPVHVAQWGVYAYPTRNKNQHSLNMEIEIENETSTGQTLTV
                     VNELLDKNKKVVARSSRKIQTPANQSIKLTENLKVKNPSLWSLEIPYLYELRTTLLQN
                     GKVIDSSTVNTGFRYFTFDPDKGFALNDTWMKVKGVCLHHDAGVLGSAVYRDVWKRRL
                     LNLKEIGVNAIRTSHNPQAPDIYQLCDELGMLVIDEAFDEWEFPKRKWIEGWNKGMPG
                     FQGTFDFFEEWGEKDLADMVRRDRNHLSVFAWSVGNEVDYPNDPYSHPILEGDKEGFT
                     QPIFGGYKKDAPDAMRLGAIAKKLVAVVKQYDKSRPTTAGLAGVAMSNETEYPGALDI
                     AGYNYTESMYDRDHKKYPQRVIFGSENRHELSAWKAARDKDFIFGQFLWTGIDY"
     gene            complement(1778..2905)
                     /locus_tag="PLN75_04295"
     CDS             complement(1778..2905)
                     /locus_tag="PLN75_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRG03371.1"
                     /translation="MNRIHYLSFLLIIFFFSSCAAKKVVSQKEEVISIIQQVNNYWQK
                     NNPVHGRAFWDNAAYHTGNMEAYFVTKNPEYLKFSEDWAIHNEWKGAKSNDKSKWKYT
                     YGETDEYVLFGDFQICFQVYADLYMIQPDPVKIARAIEVMEYQMSTSQNDYWWWADGL
                     YMVMPVMTKLYKITRNEVYLQKLKEYLNYSDSIMYDEEARLYYRDAKYVYPKHKTLSG
                     KKDFWARGDGWVFAGLAKVLNDLPPNHADRNRYVSRFKAMAESIAACQQPEGYWTRSM
                     LDAAHAPGPETSGTAFFTYGLLWGMNNRILDKSVYQPVVEKSWNYLTKTALQSDGKVG
                     YVQPIGEKAIPGQVVDANSTANFGVGAFLLAASEMVRYIKK"
     gene            complement(2947..4011)
                     /locus_tag="PLN75_04300"
     CDS             complement(2947..4011)
                     /locus_tag="PLN75_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG03372.1"
                     /translation="MNAFHPGEIWPDDSGVHINAHGGGVLYHNGTYYWFGEHKSENSN
                     TALVGVNCYSSADLYNWEKEGVALAVENDENSEIVKGCIIERPKVIFNKLTGKFVMWF
                     HLELKGQGYAAARSGLAVSDKVTGPYTFIRSERINAGKYPLNISHSVHQLNTEDKKYE
                     KWWTPEWKTAVEEGLFVKRDLRSGQMARDMTLFVDDDEKAYHVYSSEDNLTLHIAELT
                     DDYQSHTGRYIRVAPAGHNEAPAILKRNGTYWMITSGCTGWEPNEARMFSAESILGPW
                     KQHPNPMRGEKSEVTFGAQSTYIQQVYGKKDAYVFMADMWRPRFPIDGRYMWLPVRFE
                     NDIPYIEWSDSWNLNVFEGE"
     gene            4332..4877
                     /locus_tag="PLN75_04305"
     CDS             4332..4877
                     /locus_tag="PLN75_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRG03373.1"
                     /translation="MNTFDLFILIPIALGLIFGLFKGFVKEVVSLAAVVLAIIAAEML
                     SDSIKPLIAGFFDFSDKMAKTVSYILIFTLTILAALLISRLTDKLLSSISLGWLNSLL
                     GGVFGALKIAIVVSVLMNVYDALDSKFGFANPEKKEASLSYYPILKLAPSLWEEAKKT
                     IDKSKESNPEKTEEPTENIDV"
     gene            4874..5497
                     /locus_tag="PLN75_04310"
     CDS             4874..5497
                     /locus_tag="PLN75_04310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRG03374.1"
                     /translation="MSKLNPPRLIANFSGKILECGCDEAGRGCLAGPVVAAAVILPEG
                     FNCPGLTDSKQLSENMRNKLRPIIEKEALAWNVALADANEIDEINILNASILAMHRAI
                     DNLELRPEYIIVDGNRFRKYGEIPHHTIVKGDSRYLSIAAASVLAKTYRDEIMCKLHE
                     EFAGYGWKKNKGYPTVAHRKAITKYGPSPFHRMSFRLLKEPELPLDF"
     gene            complement(5494..6951)
                     /locus_tag="PLN75_04315"
     CDS             complement(5494..6951)
                     /locus_tag="PLN75_04315"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRG03375.1"
                     /translation="MMNLYKLIIGMMIFVLNFPFIQIQAQHKNRTDNNVEYLPIPEIF
                     PNDSLIAESPEKPVKTYKYILLKPSGPTDTTIFFVDSITRKHRLDSIMSIPLSKTDSL
                     LYASNSLLLPLVYTGKDIRAVWDINKNLMSLANGSRNDLITNKLPVKMTTENFVADLR
                     RGARDYISNHAMQLYVTTLDRLPSLSTFMSRPIKGSRVNPLAVYEDKIDLGNKKIEIN
                     DLKYIYWTKKANALLQFSQNYVSSNWYQGGNSNFAFLSIITGETNFDNRKNIQWDNRF
                     EWRTGLSSIIGNTKRKVMINDDILRYNTKFGLKAGGNWFYSVSGELSTQLFDSYKSIN
                     SDEYKGRLFTPVRANLGVGMDYKYKKYFSLMIAPVAFKYIYVHDTIHVNQNLFGVKKG
                     ENQLKQIGSSFRAQLNIVPARNWNLDSKLSFYTDYKKVEIDWEIVNNFIINRFLTTRI
                     SLNPRFDNTIILKEGEKTKIQFKELMSIGFSYRFL"
     gene            complement(7006..8355)
                     /gene="rmuC"
                     /locus_tag="PLN75_04320"
     CDS             complement(7006..8355)
                     /gene="rmuC"
                     /locus_tag="PLN75_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRG03376.1"
                     /translation="MEILTLLLVFFVAILAGFFAAWLYFSSKLNRVKETSAAALLQWQ
                     KEMNVAENKHVVATETLRLKTEELNKLYQENDKLTQTADERGMELAGLQIANANLMEK
                     LQTQKQELEVLQKRLTSEFENIASRILKEKASDFTESSHTNLSRILDPLKEKITLFEK
                     KVEETYDRELRDKISLKEEIVRLTELNARVSDEARSLTKALKGDVKKQGNWGEVILER
                     VLERSGLTRGQEYDIQVDVMSHEEQRQRPDVIVHLPDEKHIIIDSKVSLNAYEQFVAA
                     ETPEEQSVHLKKHILSLRTHIKELSVKNYQQAISVRTPDFVLMFLPIEASFSVAVQYD
                     NELYTYAWERKVVIVSPTTLLATLRTVSSIWRQENQTKNAQEIARLSKALHDKFLLFA
                     EDMDRIKINLDRASAAYSDALKKMKDGKGNIIRTAERIQELGGFDTQKALPTDFELF"
     gene            complement(8378..8590)
                     /locus_tag="PLN75_04325"
     CDS             complement(8378..8590)
                     /locus_tag="PLN75_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03377.1"
                     /translation="MPKNRPSKPTQNRVSLRKHRHIFTLNDLENKALNRYLSKYSVKN
                     KSKFIRETLMVEIIRRLEKDQPTLFD"
     gene            8693..9058
                     /locus_tag="PLN75_04330"
     CDS             8693..9058
                     /locus_tag="PLN75_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRG03378.1"
                     /translation="MAIHNELGNEGEIRAQAYLREHGYKIKQTNWHAGKYELDIVAEK
                     DGWLVIVEVKTRSTETFEHPQEAITLRKIKNIVNAAHHYIFQTDWQGETRFDVISVIP
                     KGQNFIIEHTEDAFLPPVE"
     gene            9196..9831
                     /gene="pyrE"
                     /locus_tag="PLN75_04335"
     CDS             9196..9831
                     /gene="pyrE"
                     /locus_tag="PLN75_04335"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRG03379.1"
                     /translation="MKLIEKNIAERLLKIKAVKLQPNNPFVWASGWKSPIYCDNRKTL
                     SYPAIRTYIKNALVRTIMDLYPEAEVIAGVATGAIAQGALVADELGLPFIYVRSSPKD
                     HGLENLIEGDLRPKQKVVVIEDLISTGGSSLKAVEAIRKDGSEVLGMVAIFTYGFAAA
                     EEKFKSAKVKLSALSNYDAVLTEALATSYIQEKDMETLREWRKNPSAWKKD"
     gene            9852..10286
                     /locus_tag="PLN75_04340"
     CDS             9852..10286
                     /locus_tag="PLN75_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRG03380.1"
                     /translation="MTTYESEIKTISQPQEMVFNTLSDLTNLNKIKNTDTEGQKMAGE
                     YFKDIESDQDSIKFSIPGFGQAGFRIVEREPFKTIKMEAENSPITAFGWIQLVPVSEN
                     TCKMKITIKADLPTMIKMMMDSKLKKGVNTIADAIAAAVSAL"
     gene            10350..11684
                     /gene="argH"
                     /locus_tag="PLN75_04345"
     CDS             10350..11684
                     /gene="argH"
                     /locus_tag="PLN75_04345"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRG03381.1"
                     /translation="MKLWEKSTEINEKIDKYTVGHDREMDLFLAPYDVLGSIAHIRML
                     ESVGLLHSEELNALEAELKIIFQIAENGEFVIEEGVEDVHSQVELMLTQKLGDVGKKI
                     HSGRSRNDQVLVDLKLFTRAEIEKTVESITALFDVLIEQSNKYRDVLMPGYTHLQIAM
                     PSSFGLWFGAYAESLADDLQLLLAAWKITNRNPLGSAAGYGSSFPLNRRLTTDLLGFD
                     SMNYNVVYAQMGRGKMERVVTTALAGVAATVAKLAYDACMFTSQNFGFIRLADEFTTG
                     SSIMPHKKNPDVFELTRARCNKIQSVPQQITFITNNLPSGYFRDLQIMKEVFVPAFDE
                     LRACLEMTTMMMDRIEVNRDILKDPKYDYLFSVEEVNRLVLSGVPFRDAYKQVGLSIE
                     AGEFTPDKNIHHTHEGSIGNLCNDEIKIYKDSIVEQFDFFKVHEAIRNLVKG"
     gene            complement(11685..12377)
                     /locus_tag="PLN75_04350"
     CDS             complement(11685..12377)
                     /locus_tag="PLN75_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRG03382.1"
                     /translation="MKRRFFHHFIDLLYPNLCLICNESLMSTENQLCLSCFSKMPKTN
                     YHLLQDNPVEKRFWGKVPVERATSFLFFQKGSDFQKLIHELKYRGNKEVGVTMGKFAA
                     ADLLDSDDFKSIDLIVPVPLHEKKEAKRGYNQSEMICNGIAEILGMPVVTDNLYRTAE
                     NTTQTRKSVFERYENTQGIFGLKNPAEFEGKHILIVDDVLTTGSTVEACIQALLQAKN
                     TRISVFTLAIAT"
     gene            complement(12441..12800)
                     /locus_tag="PLN75_04355"
     CDS             complement(12441..12800)
                     /locus_tag="PLN75_04355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRG03383.1"
                     /translation="MKRLLKSFVFAWQGIKAVFSSEQNMKIHVAVAITVIFFGFVFSI
                     SSAEWISVVICIGLVFSAEMFNTAFETLVDKVSPDKDPLAGKTKDIAAGAVLLAAIIS
                     VVVGIIIFLPKLINLIF"
     gene            12911..13429
                     /locus_tag="PLN75_04360"
     CDS             12911..13429
                     /locus_tag="PLN75_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRG03384.1"
                     /translation="MKIIQKITAVAVFLALSCVNFTSMGAFQPAQSGNNTNVFFRIHR
                     VVDGDTFWLINAKGEKEKIRFIGIDAPEAKNYGKKVKQYYGKESTNFLTNYLKGKKVR
                     LEYDVQKYDQYGRTLAYVFMEDGTFLNDYIVRCGFAVTSTYPPNVKYQSRFLKSERHA
                     RNNKLGMWAAGK"
BASE COUNT         3759 a         2944 c         2794 g         3959 t
ORIGIN      
        1 gtaatcgatt cctgtccaga gaaattgtcc gaaaatgaaa tccttgtctc ttgcggcttt
       61 ccaggcactc agttcatgtc tgttttcact tccaaaaatg acccgttgag gatatttttt
      121 atgatcgcgg tcatacatac tttccgtgta gttgtaaccg gcaatatcca acgctccggg
      181 atattcagtt tcattggaca tggcaacacc tgccagtccg gcggttgtag gtcgtgattt
      241 gtcgtattgt ttcacaacgg caaccagttt tttggcaata gcacctaatc tcatggcatc
      301 aggagcatct tttttataac ctccgaaaat tggttgagta aatccttctt tgtcaccttc
      361 cagaataggg tgtgaatagg gatcgttggg atagtccacc tcattgccta cgctccaggc
      421 aaaaaccgat aaatgatttc gatcccttct taccatatct gccaggtctt tttcgcccca
      481 ttcttcgaag aaatcaaaag taccctgaaa gccgggcatt cctttattcc atccttcaat
      541 ccattttctt ttgggaaatt cccattcgtc gaatgcttcg tcaatcacaa gcatgcccag
      601 ttcatcacat agctgataaa tatcaggtgc ctgaggatta tgacttgtcc gtatcgcatt
      661 tacacctatt tccttcaaat tcagcagcct gcgtttccat acatcccggt aaaccgccga
      721 acccagcaca ccggcgtcgt gatgcaggca cacacccttc actttcatcc acgtatcatt
      781 cagagcaaaa cctttgtcgg ggtcaaacgt aaagtacctg aatccggtat tgacagtgga
      841 tgagtcaatt acttttccgt tttgcagcaa ggttgttctc agttcgtaca aataaggaat
      901 ttccaaactc cacaacgaag gattttttac tttcaaattt tctgtaagtt taatcgactg
      961 gttagccggt gtctgaattt tcctgctgct tctggcaact acttttttgt ttttatccaa
     1021 aagttcattc accacggtca gcgtttgtcc ggtagatgtt tcgttttcaa tctcaatctc
     1081 catgttgagt gagtgttgat ttttgtttcg ggtcggatag gcatatacac cccactgagc
     1141 aacatgcacc ggatttgaat aaaccgccca aacgtttctg taaattcctg aaccggtgta
     1201 ccagcgcgaa tcagccgagc ggctgtgatc aacccgtaca gctatgacgt tatcttcacc
     1261 aaaaacaaca tagggtgtgg catcataaag gaaagaaata tatccattgg ggcgtttgcc
     1321 gatagaatga ccattgacaa acacttcgct ccggttgtaa acgccttcaa agtaaagata
     1381 gactttttcg ttttctcttt ctttgggtat atttaatgtt ttacgatacc agcctatccc
     1441 tccgggcaga taacctgtgc agcttgccag tgttggactt agcagacctt tcacactcca
     1501 gtcgtgcggt aagtcaattt tctcccagcg cgaatcgttg aaattctttt cctctccatt
     1561 ctttacatcc tgaaggataa aacgccagtt ttcgttgatt ttctccggtt taccaaatga
     1621 aacttgccca actgcaagaa caggcaacat gaaaatcagg aataagaaaa tagcagtctt
     1681 tttcatagct ataatataat tttatgtgaa attaatactc ccgaatggct caggaaattc
     1741 gattgggtta atagtttttt ttgatttaaa atctgtttta ctttttaata taccgaacca
     1801 tttcgcttgc tgccagaaga aatgctccta ctccaaaatt agccgtggaa tttgcatcca
     1861 ctacctgacc gggaattgct ttctcgccta ttggctgtac ataaccaact tttccatcgg
     1921 actgtaacgc cgttttagtc agataattcc atgatttttc gacaacgggt tgataaactg
     1981 acttatcgag aattctgttg ttcattcccc agagcaaacc gtacgtaaaa aatgccgttc
     2041 cactggtttc aggtcccggc gcatgtgcag catcgagcat gcttctggtc cagtagcctt
     2101 ccggttgctg acaggctgct attgattctg ccattgcttt gaagcgtgaa acataccggt
     2161 ttctatccgc gtgattcgga ggcaaatcgt tcaacacctt tgccagacct gcaaataccc
     2221 atccatcgcc ccgtgcccag aaatcttttt ttccggaaag tgttttatgt ttcggataaa
     2281 catatttggc gtctctgtaa taaagacggg cttcttcgtc gtacatgata ctgtccgaat
     2341 agttaagata ctctttcagt ttctgaagat acacctcatt gcgtgtgatt ttatataatt
     2401 ttgtcatgac aggcattacc atgtaaagtc cgtcagccca ccaccagtaa tcgttttgcg
     2461 aagtgctcat ctggtattcc atcacttcga ttgccctggc tatttttacc ggatcgggct
     2521 gaatcatgta aagatcggca tacacctgaa aacaaatctg aaaatcgcca aagagcacgt
     2581 attcgtcggt ttctccgtag gtatatttcc atttcgattt atcgttggat ttggctcctt
     2641 tccactcatt gtgaattgcc cagtcttcag aaaatttcag atattccgga ttctttgtga
     2701 caaaataagc ctccatgtta cctgtatgat acgcagcatt gtcccagaaa gcccgtccgt
     2761 gtacaggatt atttttctgc cagtagttgt tcacttgttg aatgatggat ataacctctt
     2821 ctttttgtga aacgactttt tttgctgcac aactgctaaa aaaaaatatt ataagaagaa
     2881 aagataaata gtgtattcta ttcattatct gataattatt ttaacttttc aaagttctgc
     2941 gtttttttat tctccttcaa acacattcaa attccagcta tcgctccatt cgatatacgg
     3001 aatatcattt tcgaaccgga cgggaagcca catgtatcgt ccatcgattg gaaagcgggg
     3061 ccgccacata tcagccataa aaacataagc atctttcttg ccgtacactt gctgaatata
     3121 ggtgctttga gctccgaagg taacttctga cttttcgccc ctcattggat ttgggtgctg
     3181 cttccacggt ccgaggatgg attcggcaga aaacatccgt gcttcatttg gttcccaacc
     3241 cgtacatccg gaagttatca tccagtatgt tccatttctt ttgagaatgg caggagcttc
     3301 gttgtgaccg gcaggtgcta cccggatata ccttccggta tggctctgat agtcatcggt
     3361 aagttctgct atgtgtaatg taagattatc ttctgaagag taaacgtggt atgccttttc
     3421 atcatcatct acaaaaagtg tcatatctcg cgccatctgc ccactcctga gatctctttt
     3481 tacaaacaaa ccctcttcaa cggctgtttt ccattcggga gtccaccatt tttcgtattt
     3541 tttgtcttct gtgttcaatt gatgcaccga gtgagatatg ttgagcggat attttccagc
     3601 gtttattcgc tccgaacgta tgaaagtgta gggtccggtc accttgtcgc ttacagccag
     3661 acccgatctg gcagctgcat atccctgccc tttcagttcg aggtgaaacc acatgacgaa
     3721 cttcccggta agtttgttga aaatcacttt tgggcgttca atgatgcatc ctttcacaat
     3781 ttcgctgttt tcatcatttt caactgccag tgctacaccc tctttttccc agttgtacaa
     3841 atcggcagac gaatagcagt taactccaac gagcgctgtg ttgctgtttt cactcttgtg
     3901 ctcgccaaac cagtagtacg taccattatg atataaaaca ccaccgccgt gcgcattgat
     3961 atgaactccg ctgtcgtcag gccagatttc gccggggtga aatgcattca atttttgttt
     4021 ttcatgaaat gcttgaatat aatcaacatt gccccggagc ggtagcgcag ttaaacacaa
     4081 taaacagcct gtaaaaacaa atatttgtat gtgtagatta catttttgca tgctgctgtt
     4141 tttctttgag tgaataagtg caaaattaat caaattagca tatttaccta aaaaagaaaa
     4201 aatcaaaaag gtagattatt ctttcaaaga aaaagaaaat ccatcgatcc ggtataaaag
     4261 tgtaattaat aacagctgct tatttttgtg tatgcgaatt tttcaccata tttgcatctc
     4321 tgaataatga tatgaataca ttcgatttat ttatccttat ccccattgca ctgggtctga
     4381 ttttcggcct tttcaagggt tttgtcaaag aagtggtatc actcgctgct gttgttttgg
     4441 caattattgc tgccgaaatg ctcagcgatt ccatcaaacc gctgattgcc ggtttttttg
     4501 atttttctga taaaatggcc aaaaccgtat catacattct cattttcacg ctcactattc
     4561 tggctgcact gcttatttcg agacttaccg acaaattgct ttcgagtatc agccttggat
     4621 ggctcaattc gctgctgggc ggagttttcg gtgcgctgaa aatagccatc gtggtaagtg
     4681 tactgatgaa cgtttacgat gcactcgaca gtaaattcgg ttttgcaaat ccggaaaaga
     4741 aagaagcctc gctgagttac tatccgattc tgaaattagc accatcgctc tgggaggaag
     4801 ccaaaaagac gattgataaa agcaaggaaa gcaatccgga aaaaacagaa gaaccaacgg
     4861 aaaatattga tgtatgagta aactgaatcc cccgcgcctg atagctaatt tctctggtaa
     4921 gatacttgag tgtggatgcg atgaagccgg tcgcggatgc ctggccggac ccgtagtggc
     4981 agctgctgtg attttacccg aaggtttcaa ctgcccggga ttgaccgact ccaaacagct
     5041 aagcgaaaat atgcgtaata agctccgacc catcatcgaa aaggaagcgc tggcatggaa
     5101 cgtggcattg gctgatgcca acgaaattga tgaaattaat atcctgaacg catcaatact
     5161 ggctatgcat cgggcaattg acaacctgga gttaagaccc gaatatatta ttgtagatgg
     5221 aaacaggttc agaaaatacg gtgaaattcc acaccatacc atcgtcaaag gcgactccag
     5281 gtatctttcc attgctgcgg catcggtgct ggccaaaacc taccgcgatg aaataatgtg
     5341 taaactgcat gaagaatttg cagggtatgg ctggaaaaaa aacaagggat atcctacggt
     5401 agcacatcga aaagctatta caaaatatgg tccaagccct tttcaccgga tgtcgttccg
     5461 attgcttaaa gagccggaac tgccgttgga tttttagaga aatcggtatg aaaaaccgat
     5521 actcatcaat tctttgaact gaatttttgt tttttcacct tctttcagga tgattgtatt
     5581 gtcgaatctc ggattcaatg aaatacgggt ggtaagaaac cggttgatga tgaagttgtt
     5641 cacgatttcc caatctattt ccactttttt gtaatcggta taaaaagata gtttggagtc
     5701 cagattccag ttgcgggcgg gtactatatt cagttgcgcc cgaaacgaac tacctatttg
     5761 tttcagttga ttctcccctt tcttcactcc gaagagattt tgattcacat gaatcgtatc
     5821 gtgcacgtaa atgtatttga aagccacagg agcaatcatg agtgaaaaat attttttata
     5881 cttgtaatcc atccccacac ccagattggc tctgacggga gtaaacaaac gccctttata
     5941 ttcgtcggaa ttaatggact tatagctgtc gaaaagctgg gtggacaatt ctcccgaaac
     6001 gctgtaaaac cagttgccgc ctgctttgag gccaaatttg gtattatagc gcagaatatc
     6061 gtcgttgatc atcactttgc gtttcgtatt gcctatgata gaactcagac ctgttcgcca
     6121 ttcgaatctg ttgtcccact gaatattttt tctgttatcg aaattcgttt caccggtaat
     6181 aatactcaaa aatgcaaaat tgctattacc tccctgatac cagttgctcg agacataatt
     6241 ttggctgaac tggagcaaag cgtttgcttt ttttgtccag tagatatatt tcaaatcgtt
     6301 gatttcgatt tttttgtttc ccaggtcaat cttatcttca tacactgcca gtggattcac
     6361 cctgcttcct ttaatggggc gcgacatgaa tgtactcaga ctcggcaacc tgtcgagggt
     6421 ggttacatac agctgcatgg cgtggtttga aatgtaatcc cgggctcccc gtctcagatc
     6481 ggcaacgaaa ttttctgttg tcattttcac tggtaactta ttcgttatca gatcattcct
     6541 gcttccgtta gccagtgaca tcaggttttt gttgatgtcc cacactgccc tgatatcttt
     6601 acccgtgtac acgagcggca aaagcaatga attggaggca tatagcagag aatcggtttt
     6661 tgagagtggt atgctcatga ttgaatcgag cctgtgtttg cgggtgattg aatccacaaa
     6721 aaagatggtg gtatcggttg ggcccgacgg tttcagcaaa atgtatttat aggtttttac
     6781 cggtttttcc ggcgattctg cgatgagtga gtcattggga aaaatttcgg gaattggcag
     6841 gtattcaaca ttgttatctg ttcggttttt atgctgagcc tggatttgaa tgaatggaaa
     6901 attcaaaaca aaaatcatca ttccgattat taatttgtac aaattcatca tttttttctt
     6961 tagttttgaa tgtgcaaagg tacgaaatat aagtgtttag aaaactcaaa aaagctcaaa
     7021 atccgttggg agagcctttt gggtatcgaa tccgcccagt tcctgaatac gttcagcggt
     7081 tcggatgatg ttacctttgc cgtctttcat tttctttaat gcatcgctgt aagccgcgga
     7141 tgctctgtcc agatttattt ttattctgtc catgtcttcg gcaaacaaga gaaatttatc
     7201 atgcagcgct ttgctcagcc gtgctatttc ctgagcattt tttgtctgat tttcctgcct
     7261 ccagatagaa gaaacagttc tcagagtagc cagaagcgtg gtaggactca caatcaccac
     7321 tttccgttcc caggcgtaag tgtatagttc gttatcgtac tgcaccgcca ccgaaaacga
     7381 agcctcgatg ggcagaaaca tcagcacaaa gtcgggtgta cggacgctga tggcctgttg
     7441 atagttctta acactcaact ctttgatgtg cgtacgtaag gataagatgt gtttcttcag
     7501 atgtacgctc tgctcttcgg gcgtttcggc cgctacaaac tgctcatagg cattgagcga
     7561 cacttttgag tcaattatga tgtgtttttc gtcgggcaga tgaacaatca catccggccg
     7621 ttgccgctgt tcctcgtggc tcatcacgtc cacctggatg tcatactcct gcccgcgggt
     7681 gagccccgaa cgttcgagaa cccgttccag tatcacttct ccccagtttc cctgcttttt
     7741 cacatcgcct ttcagggctt tggtgaggct tctggcttcg tcgcttacgc gagcattaag
     7801 ttcggtaagc cttactattt cttctttcag gcttatttta tccctgagct ccctgtcgta
     7861 ggtttcttcc actttctttt cgaaaagcgt aattttctct ttcagcggat caagtatccg
     7921 gctcagattt gtatgactgg attccgtgaa atcacttgct ttttctttca gaatgcgcga
     7981 ggcgatattt tcaaattcgc ttgtcagacg cttctgtaat acttcgagtt cctgtttctg
     8041 agtctgaagt ttctccatca gattcgcatt ggctatctga agccctgcaa gttccatacc
     8101 acgttcgtcg gctgtctgag ttagcttatc gttttcctga taaagcttat tgagctcttc
     8161 ggttttgagg cggagggttt cggtggctac aacgtgctta ttttcagcta cgttcatttc
     8221 tttctgccat tggagcagcg cagcagcaga ggtttctttt acccggttca gtttggagga
     8281 aaaataaagc caggcagcaa aaaaacctgc aagaatagca acgaaaaaaa ccaataaaag
     8341 ggtgaggatt tccataaccg gatgttcagc tgttcatcta atcaaaaagc gtaggctggt
     8401 ctttttccaa ccgtcgaatg atttccacca tcagtgtttc gcggataaat ttggatttat
     8461 tcttcacgct gtacttgctc aggtatctgt tcaatgcctt gttttccaga tcgtttagcg
     8521 taaaaatatg gcggtgttta cgcagtgaaa cacgattttg tgtgggcttc gatggtctat
     8581 tttttggcat aatgaaaatt taacagcaca aaaataacat tatatcctta aattatcttg
     8641 tatttttttg aaaaaagtct atttttgcaa tgaatgttaa aaaaactaat tgatggcaat
     8701 acacaacgaa ctgggtaacg aaggcgaaat acgtgctcag gcatacctgc gtgagcatgg
     8761 ctacaaaatc aaacaaacca actggcatgc aggcaaatac gaactggata ttgtagccga
     8821 aaaagacggc tggctggtga tagtagaagt aaaaacccgc tcaaccgaga cttttgaaca
     8881 cccgcaggaa gccattacgc tacgtaaaat caaaaacatt gtaaacgcag cacatcatta
     8941 catttttcag accgactggc agggcgaaac ccgttttgac gttatttccg tcattccaaa
     9001 aggacaaaat ttcatcatag agcataccga agacgccttt ttaccacctg tggaataaaa
     9061 aacgccgctt aaatatcgtt taaattccat tttttaactt agtttgttga atactttgag
     9121 taattttgcg gaaaaattga gcatgtgctt ttctcatttt cactcaatta tttatgaaaa
     9181 aaaacacaca aatttatgaa acttattgaa aaaaatattg cagaaagact gttgaaaatc
     9241 aaagcagtaa aacttcagcc caacaatcct tttgtatggg catccgggtg gaaatcacct
     9301 atttattgcg acaacaggaa aacactttct tatccggcaa tccgaactta tatcaagaat
     9361 gcgttggtac gcaccatcat ggatctttat ccggaggcag aagtgatagc cggagtggct
     9421 acgggtgcaa tagctcaggg agcactggta gccgacgaat taggcttgcc gtttatttac
     9481 gtccgctcaa gcccgaaaga tcacggactg gagaatctga tagaaggcga tctgagaccc
     9541 aaacaaaaag tggtggtgat agaagacctg atatcaaccg gcggcagtag cctgaaagct
     9601 gtggaagcta tccgtaaaga cggctcagag gtgctcggta tggtggcaat atttacatac
     9661 ggatttgctg ccgccgaaga aaaattcaaa agtgcgaaag taaaactctc agcgttaagc
     9721 aattacgacg cagtgcttac agaagccctg gctaccagct atattcagga aaaagatatg
     9781 gaaactctac gtgaatggag aaaaaatccc tcagcctgga aaaaagattg attcatacta
     9841 tccaaaaaaa aatgaccaca tacgaaagtg aaataaaaac catttctcaa ccacaggaaa
     9901 tggttttcaa taccctgagc gacctgacca acctcaacaa gattaaaaac acagacactg
     9961 aggggcaaaa aatggcaggc gaatacttta aagacatcga atccgaccag gactctataa
    10021 agttttcaat tcctggtttc gggcaggcag gtttccggat tgtagagcgt gaaccgttta
    10081 aaaccattaa aatggaagcc gaaaattctc ccataaccgc ttttggctgg attcagcttg
    10141 taccggtttc ggagaatacc tgtaagatga aaatcaccat caaagccgac ctgcctacga
    10201 tgataaaaat gatgatggac agcaaactga aaaaaggggt aaataccatt gcagatgcga
    10261 tagctgcagc ggtttctgct ctctgaaagc tttatttcgg attctataat taataacaat
    10321 tctcatattc ccgttccgga gggcaaaaca tgaaactttg ggaaaaatca acagaaataa
    10381 acgaaaagat tgacaaatat accgtcggac acgaccgtga aatggatctc tttttagccc
    10441 cgtatgatgt actgggttca atcgcacaca ttcggatgct cgaatcggtt ggtttgctcc
    10501 acagcgaaga actgaacgca ttggaggcag agctgaaaat catatttcag attgcggaaa
    10561 atggggaatt tgtgattgaa gaaggcgtgg aagacgttca ctcgcaggta gaacttatgc
    10621 tcacacagaa actcggagat gtcgggaaaa aaattcacag cggtcgttcg cgcaatgatc
    10681 aggtgctggt ggatctgaaa cttttcaccc gtgccgaaat tgaaaaaacg gttgaaagca
    10741 tcacggcact gtttgatgta ctgatagagc aaagcaataa ataccgcgac gtgctgatgc
    10801 cgggatacac ccacctgcag attgccatgc catcgtcgtt cgggctttgg tttggcgctt
    10861 atgccgaaag ccttgctgat gatctgcaac tgctgctggc tgcctggaaa atcacaaacc
    10921 gcaatccgct gggttcggct gccggttacg gttcatcatt cccactcaac cgccgcctta
    10981 caaccgatct gctgggattc gacagcatga attacaacgt tgtgtacgct cagatggggc
    11041 gtggtaaaat ggagcgtgtg gtgactaccg cattggccgg agtggctgct acagttgcca
    11101 aactggctta cgacgcatgc atgttcacct ctcaaaattt cggtttcatt cggctggctg
    11161 atgaatttac caccggttcg agcattatgc cccacaagaa aaatccggat gtattcgaac
    11221 tgacacgtgc ccggtgtaat aaaattcaat ctgttccgca gcagattacg ttcattacca
    11281 acaacctgcc ttcgggctat ttcagagacc tgcaaataat gaaggaggtt tttgtgcctg
    11341 ctttcgatga attacgtgcc tgcctcgaaa tgacgaccat gatgatggac cggattgagg
    11401 taaaccgcga tattctgaaa gatccgaaat acgactacct gttcagtgtc gaggaagtga
    11461 accggctggt gttaagcgga gtaccattcc gcgacgccta caagcaggta ggcctgagca
    11521 ttgaagcagg tgaatttaca cccgataaaa acattcatca cacccacgag gggagcattg
    11581 gtaacctctg caacgatgaa atcaaaattt acaaagacag tatcgtcgag cagtttgatt
    11641 ttttcaaagt tcacgaagct atccggaatt tagtgaaagg atgattaagt agcaatggcg
    11701 agtgtaaata cgcttattct tgtatttttg gcctgcagca atgcctgtat acatgcttcg
    11761 acggtggagc cggtggtgag cacatcgtca acgattaaaa tgtgtttccc ttcaaattcg
    11821 gccggatttt tcaatccgaa aataccctgt gtattctcgt atcgctcaaa aaccgattta
    11881 cgggtttgag tggtattttc agctgttctg tacagattgt ccgtcaccac cggcattcct
    11941 aatatttcgg ctatgccgtt acaaatcatt tcgctctgat tgtaaccccg tttggcttcc
    12001 tttttttcat gcagcggcac cggcacaatc agatctatcg atttaaaatc atccgaatca
    12061 agtaaatcag ctgccgcaaa ttttcccatt gtaacgccca cttctttatt tcccctgtat
    12121 ttcagttcgt gaatcaattt ttgaaaatca gatcctttct gaaaaaacaa aaaagaagtt
    12181 gctctttcaa ccggcacttt accccagaat cttttttcta ccggattgtc ctgaagcaaa
    12241 tgataattcg ttttgggcat tttactgaaa cacgacagac agagctggtt ctccgtactc
    12301 atcaggcttt cattgcagat aaggcaaaga ttgggataca gcagatcaat aaaatgatga
    12361 aaaaaacggc gtttcattcg gttaatcatt aaattcagga cagttttgac gaaaaaaatc
    12421 tgtaccttta aaaatcattt tcagaaaatc aggtttatca gtttcggaag aaaaatgatt
    12481 attcccacca caacagagat aattgccgcc aacaaaactg ctcctgcggc tatgtccttg
    12541 gtttttccgg ccagagggtc cttatcgggc gaaaccttgt cgacaagtgt ttcgaaagcc
    12601 gtgttaaaca tctcggcgct gaaaaccagt ccgatacaaa ttacaaccga aatccactca
    12661 gctgaagaaa tgctgaagac aaagccgaaa aaaatcaccg taatagccac tgcaacgtgt
    12721 attttcatgt tttgctccga agagaaaacg gcctttatcc cttgccaggc aaatacgaaa
    12781 ctttttagca atcttttcat gtcattgatt tgaatatatt cagatacaaa tgtatgaaaa
    12841 tctgagcaag tttcaaattt aaaatgaatt tgtaactttg taattattat tttatcaact
    12901 cgttacgaca atgaaaataa tacagaaaat tacggccgtt gccgtttttc ttgccctatc
    12961 atgtgtaaat ttcacctcta tgggtgcttt tcagccggct caatccggaa acaacacgaa
    13021 tgtcttcttc agaattcatc gagtagtaga tggcgacact ttctggctga ttaacgcaaa
    13081 gggcgaaaaa gaaaaaatcc gttttattgg catcgatgct cccgaggcaa agaattacgg
    13141 caaaaaggta aagcaatatt atggaaagga gtcaacaaat tttttaacaa attacctgaa
    13201 aggaaaaaaa gtaagactgg aatacgatgt gcagaaatat gatcaatacg gccgaacgct
    13261 ggcttatgtt ttcatggaag acggcacttt tctgaatgac tatatcgtac ggtgcggttt
    13321 tgctgtcaca tccacctatc cgcccaatgt gaaatatcag agccggtttt tgaaaagtga
    13381 aagacatgcc cgcaacaaca aattgggaat gtgggcagca gggaagtaaa aaaccacaaa
    13441 aaaaatccgg taaaaa
//
LOCUS       DAORKP010000051        13320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_417_length_13320_cov_9.294685, whole genome shotgun sequence.
ACCESSION   DAORKP010000051 DAORKP010000000
VERSION     DAORKP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_13320_cov_9.294685"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..4471)
                     /locus_tag="PLN75_04365"
     CDS             complement(56..4471)
                     /locus_tag="PLN75_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRG03385.1"
                     /translation="MLNISKVQNYVAQRITKSLSEKLQTTVSVGSVDYRLFNTFKFND
                     LYIQDLKKDTLLYARSGYLDFNFRRLFEGKIIFNELRLDRLEGNLVTDTAGINNLDFI
                     IRAFKKPKKKQEPSPVEFNFRDIRITNSSFKLHNLQRKALSDAGKFDINRLTINDINA
                     RIAVDYFSGDSLKATILSLSAREKSGLTVENLSTQIQGFKNGFRFPLLTLKLPDSNLE
                     LDSVRMDYDSISGLSDIRNKVRWHARIKPSNVVLKDLSAFVPGFSTIKDPVKIQGRLS
                     GRISSFRLQDLEFQYRNSFSMRANVDMNGIPDVEETFIYADIRDFSINKGEAQDLISK
                     LTRKPFVLPKELSRLGTVKYQGNISGFFSNLVAYGNVRTDVGSLKTDILLQLKNNMRD
                     ILYNGTVESGSFQLGTLLNTKTLGKTAFKINTKGSKLQNKSLQGALSGSIPEIFLNQY
                     TYRNITLDGNYDGTGFEGSVNIADPNLTADFDGMVDLTHKLPVFNFDLLVEKADINKL
                     KITDKYEDALLSFRGNTNMVGNSLDNLNGYLMLDSILFVNKGKTLDMKLLLFESEVGD
                     GSKFTITSDLINGFFDGNFKYSTIPQTVTSILQNYLPALSGKNVIRNRKTNGGDNQMD
                     IDLTINDTKLLSEVFELPFTLDGTTSVKGFLDEKNNQLEIKAETPFLSFGKRRIQDLF
                     VSLNNEKNQLNLLANGRYNFAKDFLSLNLKAAAKNDSLYTQVGWQNNDSVVNAGELQA
                     SAKLFKENDLTSALIDFKPTQIILFDSVWDVSPSQIAVNPDSTLDVKKFRIQNNNQYI
                     LIDGLLSKHNSDVLDVEMNEIEIDYILDLFKFKALTIKGKASGIASIYGVLSKPVFES
                     NLFVKDAYLNESLLGDAYIYTGWMRDNSELLVSGTFLQNKDTVALANGVYSLKNDSID
                     FMFDADKMNLGFLQQWLGGIVQNVSGLGTGSVRMFGPTKNIGFEGSVLAENTKLTIDF
                     LKTTYSFTDSIRLTRKSIEMKDITVYDAERNKGVLNGIVKHDGSFKNYEYDFEISARN
                     LLALDTKATDNDFFYGKAYATGDVHIYGEDEYTNIDIDAISQPKTKMYISVGGASIAN
                     ENDFITFVNPRDSAGLASKQKPESPKNEFFMNLTMNMDITPDADIQLIIDPQAGDMLA
                     ANGTGNLRLEYDDRTDMKLYGDYTISKGTYLFTLQELLRKQFKIDQGSTITWSGDPLR
                     AQLDIRAIYSLTASLRDLMDQNILSSTGRSSVPVNTVLNLTDELTRPTIKFDIDLPSS
                     DETLKMQVRNLINTEEMMNRQMVYLLLFNKFYTPEYNRTASSFASTTGSTISSFLSNT
                     AFGQLNNWLSQFSSNFSLGVNWRSSGYGSTMTNEYEGEIMYQPNNRFIVNGNIGYRDD
                     NFTKNKIIGDLDVEYLLTENGKYRLKAYNHTVDRYSLRAAPFIQGVGIVYKEEFNSWG
                     ELWRRYLEAFNSTRTVQDSVPLEIPALKPDTIKSE"
     gene            4791..5825
                     /gene="tsaD"
                     /locus_tag="PLN75_04370"
     CDS             4791..5825
                     /gene="tsaD"
                     /locus_tag="PLN75_04370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRG03386.1"
                     /translation="MQKNITVLGIESSCDDTSAAVIRDGVMLSNITAGQKVHEKYGGV
                     VPELASRAHQQNIIPVVDTALKEAGIEPEELTTIAFTRGPGLLGSLLVGTSFAKGMSQ
                     ALNIPLVEVNHLQAHVLAHFISLGAENEEFPQFPFLCLLVSGGNSQIVLVRDYPDMEV
                     IGQTIDDAAGEAFDKCAKVMGLPYPGGPHVDRLAKEGNPKAFKLNKPQINGYDYSFSG
                     LKTSFLYLLRDKLKENPDFVTENMNDLCASLQATVIDILMHKLLKASQDLGIKQVAVA
                     GGVSANSALRQAFHDYAATYGWQVYIPPFSFTTDNAAMIAMTGYFKYLKGDFCDLNET
                     PYARVSNLLA"
     gene            5900..6466
                     /locus_tag="PLN75_04375"
     CDS             5900..6466
                     /locus_tag="PLN75_04375"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HRG03387.1"
                     /translation="MKNLLPEKLRPFSSVIYFMIILVVSHFFWKFFVIGDESNEQVTF
                     FGWNISQPFNYMSRHIAVITHSLLDRIGYDITLNSNNVIRHNISKNAVWIVWSCTGIK
                     QAYIFFCIIAFYRGPWLHKLWYIPVGIVLVYLFNIFRITIITIIIDKYPDQFELWHEH
                     ILKYAFYGLIFLIWALWNEKFVEKKPLS"
     gene            complement(6521..7582)
                     /gene="buk"
                     /locus_tag="PLN75_04380"
     CDS             complement(6521..7582)
                     /gene="buk"
                     /locus_tag="PLN75_04380"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRG03388.1"
                     /translation="MHKILVINPGSTSTKVAVYEDDKQTELFSVRHSVEKLMEFPHVI
                     DQFDFRYELIMNSLREKGLALDFSIIMGRGGMLPPVQSGVYEVNERMLAALRKAERGE
                     HASNLGAFLADKIARQIPGCKAYIADPVAVDELDDVARVSGLPEIPRQSIFHALNHKA
                     AGRYHAAKVGKKYEELNLVIAHLGGGVTVAVHRQGRVVDVNQGLDGYGPISPERAGTL
                     DNGRLVRLCFSGKYTENELLRKLAGKGGLIAHVGSNDVQELVKKAEGGDTHVASMLHA
                     MSYTIGKEIGAMLAVLKGKADGVILTGGVAFNDYVVRDIKEMIEPYAAVFVYPGEDEM
                     TALAANGLRVINGEKAKIF"
     gene            7982..9307
                     /locus_tag="PLN75_04385"
     CDS             7982..9307
                     /locus_tag="PLN75_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRG03389.1"
                     /translation="MPNSLKTYTLSNYKLIPQISGLSDEMIEAIEVVGRVLPFKTNSY
                     VVNELIDWTQIETDPMFTLTFPRREMLETRHYQTVKKLLDENTDKALLDRKIHKIRME
                     LNPNPAGQEHNVPEMNSIKLKGIQHKYPETVLFFPSQGQTCHAYCSFCFRWPQFSGMS
                     GFRFAMKEAELLKQYLIKNKKVTDLLFTGGDPMTMSSRLLAGYIEPLLEPEFDHIRSI
                     RIGTKSLSYWPYRFLKDDDSDDIIRLFEKIIQSGKNLSIQAHFNHPVELSTDAVRKAI
                     SRIRSTGAQIRTQSPLLRHINDKPELWAEMWRKQVDLSCIPYYMFIARDTGSKRYFEL
                     PLEKCWDIFRKAYRQVSGLCRTVRGPSMSCSPGKVQVLGVQEVMGEKAFVLRFLQGRK
                     PEWVDVPFFAAYDPKATWFDELEPAFGEKKFFFEKNEATKPRDPEFLFE"
     gene            complement(9459..10772)
                     /locus_tag="PLN75_04390"
     CDS             complement(9459..10772)
                     /locus_tag="PLN75_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRG03390.1"
                     /translation="MKNKIFRYLSYFNIAGEREDFETIHSEVEKGVVFKGTNLWVLIC
                     AILVASVGLNMNSTAVIIGAMLISPLMGPINGMGYSLATYDFLLFRKAIKNFTFAVVV
                     SLITSAFYFFISPVNTAHSELLARTSPTIYDVFIALFGGVAGMLALSSKLKGNVIPGV
                     AIATALMPPICTAGYGLATFQFSFFFGALYLFTINTVFIAVAALFVSQVLKFPIRTIV
                     DKERARRVNHYITFILLVTAIPSVYFGYKLVQNEDFADKARNLIATVSVQENSYLFDS
                     NINPSNKSIRLIYSGYGVSNQTKTDIKKTALELNLDTAKIEILSGTDQSQLHSETQRR
                     LTEVETLNNQLTSTGFSLRQTQNRLDSLENIQNKGKEILAEVREFYPQITKCSYSVTY
                     IHPDTVKTPIVIFNVKRNLSPSDREKVTNWTKSRVKNMNAKVYFD"
     gene            complement(10933..11496)
                     /locus_tag="PLN75_04395"
     CDS             complement(10933..11496)
                     /locus_tag="PLN75_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRG03391.1"
                     /translation="MFDFLDQYPYLLPVMIFFGRICDVTLGTLRIIFVSKGKKGIAPV
                     IGFFEVFIWIVIISQILSRASDLIAYLSYAGGYATGTYVGILIENKLAFGVLLYRVYT
                     KENGSTLVQLLNANGFGATLVRGEGSVGKVDVIETVVARQLMKKIDKLIKEFDENAFY
                     VTEDVRTAQNGIFPKTASIFYRWRPGK"
     gene            complement(11588..12994)
                     /locus_tag="PLN75_04400"
     CDS             complement(11588..12994)
                     /locus_tag="PLN75_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG03392.1"
                     /translation="MLSDFISRKIREEFGFEPTGQQSVLIDLLGAFIMSADSEKAFLL
                     KGYAGTGKTSIVSALVRAMNALQQKTVLLAPTGRAAKVLSAYSGFPAYTIHKKIYRQK
                     SITDFRFQLSDNLHKNTLFIVDEASMISNTRLDASFGSGRLLDDLIEYVYSGDGCSLL
                     LLGDTAQLPPVEQTQSPALETEALKSFGLHVDEFELTYVVRQALESGILFHATNLRMH
                     LAQETTGEHPKFKLNTFTDIQRLAGMELIDTIQRSYSGVGVEDTIVITRSNKRANLYN
                     DGIRSRVMMREEQLSNGDLLMVTRNNYFWNKPYPEIDFIANGDVLEVTRIRKHREMYG
                     FRFADLSLRSLDYGWEIDARVWLDGLTAENPAQMNEMTNRLFTAVAEDYPEITNRRQL
                     VRKVLENEFFNALQVKFAYAVTCHKAQGGQWKKVFIDHGFLPDDQINAEYFRWLYTAL
                     TRATDQVYLVNFPDEWFV"
BASE COUNT         3660 a         2903 c         2824 g         3933 t
ORIGIN      
        1 gctatcggga cttcattctc atgggtgaaa atttgtgttt aatggctgtt tcatattatt
       61 ctgattttat tgtatctggt tttaacgcag gaatttccaa aggaacgctg tcctgcacgg
      121 ttcgggtgga attaaacgct tccaggtagc gccgccacaa ttcaccccag gagttaaatt
      181 cttctttata aacaatacct actccctgga taaacggtgc tgcccgcagc gaatacctgt
      241 ccacggtgtg gttgtaggct ttgagccggt atttaccgtt ttcggtgagc aggtattcca
      301 catccagatc gccgataatt ttatttttcg taaagttatc atcgcgatag cctatattac
      361 cgttgacgat aaaacgattg ttcggttgat acatgatttc accttcgtat tcgtttgtca
      421 tggtggagcc gtatccgctg cttcgccagt tgacacccaa tgagaaattg gatgagaact
      481 gtgaaagcca gttgttcaat tgtccgaatg ccgtattgga tagaaatgag gaaatggtgc
      541 ttccggtagt cgatgcaaaa cttgaggcag tacggttgta ttccggagta taaaatttat
      601 tgaaaagcag cagataaacc atctgccggt tcatcatttc ttcggtatta atcaggttgc
      661 gaacctgcat tttcagggtt tcgtcgctcg acggaagatc aatgtcaaat tttatagtcg
      721 gccgggtaag ttcatcggtg agattaagca ccgtattcac gggcacactg ctccggccgg
      781 tcgacgagag gatattctga tccatcaaat cgcgcaatga cgccgtgagt gaatagatag
      841 cgcggatatc gagctgagcc cgaagcggat cgcccgacca ggtgatggtg ctgccctgat
      901 caattttgaa ctgttttctt aacaattcct gtagtgtaaa aagataagtt cctttagaaa
      961 tggtgtaatc gccgtaaagt ttcatatccg ttcggtcatc gtactccagt cgcaaattgc
     1021 ctgttccgtt ggcagcaagc atatcacctg cctgaggatc aataatcagt tgtatgtcag
     1081 catcgggagt gatatccatg ttcatggtca gattcatgaa aaactcattc ttcggtgatt
     1141 caggtttttg ctttgaagcc agtccggcag aatcccgtgg attgacaaag gtgataaaat
     1201 cgttttcgtt ggcaattgaa gcccctccta ccgaaatata catttttgtt ttgggttgag
     1261 atattgcatc gatgtcgata ttggtatatt cgtcttcacc gtaaatgtgc acatcaccgg
     1321 ttgcataagc ttttccataa aagaaatcgt tgtcggtagc ttttgtatcg agcgccagca
     1381 ggttgcgggc actgatttca aagtcgtatt cataattttt gaacgatccg tcgtgtttta
     1441 ctatcccgtt gagtactccc ttgtttcttt cggcatcata cacagtaata tccttcattt
     1501 cgatggattt tcgggtaagg cgtatggagt cggtgaaact gtaggttgtt ttcaaaaaat
     1561 cgatagtcag tttcgtattt tcggccagaa cactgccttc aaatccgata ttttttgtag
     1621 gtccgaacat tcgcaccgat cctgttccaa ggccactcac attttgtaca attcctccca
     1681 gccattgctg caaaaagccc agattcattt tatcggcatc aaacataaaa tcgattgaat
     1741 catttttcag cgaataaacg ccgttggcaa gcgctacagt atccttgttt tggaggaaag
     1801 tacccgaaac gagcagttcg ctattgtctc tcatccatcc ggtgtagatg taggcatcgc
     1861 ccagcagcga ttcattcagg tatgcatctt tcacaaatag atttgattca aaaaccggtt
     1921 tgctgagtac tccgtaaatg cttgcaatgc ccgaagcttt tcctttgatg gtcagtgctt
     1981 tgaatttaaa caaatccagt atgtaatcga tttcgatttc attcatttcc acatcgagta
     2041 catccgaatt gtgtttcgaa agcagtccgt cgattaaaat atactgattg ttgttttgaa
     2101 tcctgaattt tttcacgtcg agcgttgagt ccggattaac ggctatttgc gatgggctga
     2161 catcccacac ggaatcaaaa agtatgattt gtgtcggttt gaaatctatc aaagctgaag
     2221 tcaaatcgtt ctctttgaag agtttggcag aagcctgcaa ttcgccggca ttcactaccg
     2281 aatcgttgtt ctgccatccc acctgcgtgt acagcgaatc gtttttggca gctgctttca
     2341 ggttcagact cagaaagtct tttgcaaaat tgtatcttcc gttggcaagc aggttcaact
     2401 ggtttttttc attattcaga ctgacaaaca aatcctgaat tctgcgtttt ccaaacgaaa
     2461 ggaagggagt ttcggctttg atttcgagct ggttgttttt ttcatccaga aatcccttta
     2521 ccgaggtagt accatccagt gtaaaaggca gttcgaatac ttctgaaagc aatttcgtgt
     2581 cgtttatggt cagatcaatg tccatctgat tgtcgccgcc gttcgtttta cggtttctga
     2641 tgacgttttt acccgaaagc gccggcagat aattctgcag aatggaagtc actgtttgag
     2701 gtatggtgct gtatttgaaa tttccatcga agaatccgtt aatcaggtcg gaagtgatgg
     2761 taaattttga tccgtcgccc acttccgatt caaaaagcag caacttcatg tccagcgttt
     2821 ttcctttgtt gacaaaaaga atgctgtcca gcatcaggta tccgttgagg ttgtccagcg
     2881 aatttcccac catattggta ttgcctctga aagaaagcag agcatcttcg tatttatcgg
     2941 ttattttcag tttgttgata tcagcttttt cgaccagcaa atcgaaatta aataccggaa
     3001 gtttatgggt cagatccacc attccatcga agtcggcagt aaggttgggg tcggcaatat
     3061 tcacacttcc ttcgaatccg gttccatcgt aatttccatc cagcgtgata tttcgatacg
     3121 tgtactgatt caggaatatt tcgggaatac tgccgctcag agcaccttgc agacttttat
     3181 tctgaagttt tgatcctttc gtgttaattt tgaaagcggt ttttccgagt gttttggtat
     3241 tcaggagcgt accaagctga aaactgcctg attccaccgt tccgttgtaa agaatatcgc
     3301 gcatattgtt tttcagttgc aacaaaatat cggttttcag acttcccacg tcggttctta
     3361 catttccgta agctaccaga ttgctgaaga agccggaaat attcccctga tacttcacag
     3421 tgcccaatct tgagagttct ttggggagca cgaaaggttt ccgggtgagt tttgaaatca
     3481 agtcctgcgc ttcaccctta ttgatgctga aatcccgtat atctgcataa ataaaggttt
     3541 cttcaacgtc agggataccg ttcatatcca catttgcccg cattgaaaag gagttgcggt
     3601 actgaaattc aagatcctga agtctgaaac tcgaaattcg cccggatagc cgtccctgaa
     3661 ttttcaccgg atctttgatg gtggaaaaac cgggaacaaa tgccgataaa tctttcagca
     3721 ccacgttgga gggtttgatc cttgcgtgcc atcgtacttt gttccggata tcactgaggc
     3781 ctgaaatgct gtcgtaatcc atgcggactg aatcgagttc caaattcgaa tcgggcaatt
     3841 tcagggttag caacgggaac cggaaaccgt ttttaaaccc ctgaatctgc gtgctcagat
     3901 tttcaacagt caaacctgat ttttcccggg cgctcaacga caaaatggtt gctttcagcg
     3961 aatcgccgct aaaataatcc accgctatcc gtgcgtttat atcattgatt gtcaaacggt
     4021 taatatcaaa tttaccggca tcgctcaaag ctttccgttg caggttgtgg agtttgaacg
     4081 aagaattggt gatgcgaata tccctgaagt taaattcgac cggcgaaggc tcctgtttct
     4141 ttttcggttt tttgaatgcc ctgatgataa aatcgaggtt atttataccg gccgtatcag
     4201 tcaccagatt tccttccaac cgatccaaac ggagctcgtt gaaaatgatt ttcccctcaa
     4261 ataatcttct gaaattgaaa tcaagatacc cgctgcgggc atagagcaat gtgtcttttt
     4321 tcaaatcctg aatatacaaa tcattgaatt tgaatgtgtt gaaaagccgg tagtcaacag
     4381 agccaacgga cactgtagtt tgaagttttt ccgaaagact tttggtaatc cgctgtgcaa
     4441 catagttttg caccttactg atattcagca agatgaacaa agttgcaatc agcgcaatga
     4501 taccgagcaa cgtaaagaca atgatatttc ggagtttttt gatgataaag caggatttta
     4561 attacaaaag taactaaatt ttcgctttta gcattgcaaa gagcgtatta gttagtactt
     4621 ttgcagagtt ttgataaaat acacaaaaat taaaccaatc ggataagatt aagtacccgg
     4681 aatgctgcat tccacctgtg acaggataaa atcaggaggt ttacaggtaa ttgtttcaaa
     4741 caaaagcgga acgaaaatcg tttcatcagt catcgtattc tgatagaatt atgcaaaaaa
     4801 atataactgt actcgggatt gaatcttcgt gtgatgatac ttcagcagcc gttatcagag
     4861 atggggtgat gctatcaaac attactgccg gtcagaaagt gcacgaaaag tacggaggtg
     4921 tggtgcccga actggcatcg cgcgctcatc agcaaaacat tattccggtg gttgacactg
     4981 ctctgaagga ggccggaatc gagccggaag aactcaccac cattgcattt acacgcggcc
     5041 ccggactgct tggctcgctg ctggttggca cttcgtttgc caaaggcatg tcacaggcac
     5101 tcaacattcc gctggtggaa gtcaatcatt tgcaggcaca cgtgctggct catttcatca
     5161 gcctgggtgc tgaaaatgag gaattccccc aatttccgtt tctttgtctg cttgtatcgg
     5221 gcggcaattc gcagattgtg ctcgtgcgcg attacccgga tatggaggtc atcgggcaaa
     5281 ccattgatga tgctgccggt gaggcttttg acaaatgtgc caaggtgatg ggacttccct
     5341 acccgggcgg ccctcatgtg gacaggctgg caaaggaggg gaatccgaaa gctttcaaac
     5401 tgaacaaacc tcagataaac ggatacgatt acagtttcag cgggctgaag acttcgttcc
     5461 tgtatctgct gagagacaaa ctgaaagaaa atccggattt tgtgaccgag aatatgaatg
     5521 atttgtgtgc aagtcttcag gctactgtga ttgacatttt gatgcataaa ctgctgaaag
     5581 cctcgcagga cctgggaata aaacaagtgg cagtagccgg aggagtttcg gccaattcgg
     5641 ctttgaggca ggcatttcac gactatgcag ctacgtatgg ctggcaagtg tacataccgc
     5701 cattcagttt tacgaccgat aatgcagcga tgattgccat gaccggatat ttcaaatacc
     5761 tgaaaggtga tttttgtgat ttgaacgaaa ctccttatgc cagggtgagc aatttgctgg
     5821 cataattttc ataaaaacag tcaatttgaa aagccagttt ctttcatgac aactttttta
     5881 attaaaaacc taaaaactga tgaaaaatct actacccgaa aaactccggc cattcagcag
     5941 cgtgatttat ttcatgatca ttctggttgt gtcgcacttc ttctggaaat tctttgtgat
     6001 cggcgacgaa agcaatgagc aggttacctt ttttggttgg aacatttcac agcctttcaa
     6061 ctacatgtcc cggcacattg cagtaattac ccactcgttg ctcgaccgga tcggatatga
     6121 tattacactt aattctaaca acgtaatccg gcataatatc agtaaaaatg cagtctggat
     6181 tgtgtggagt tgtacgggca tcaagcaggc ttatattttc ttctgcatca tagccttcta
     6241 tcgcggtccg tggctgcaca agctttggta catacctgtt ggcattgttt tggtatatct
     6301 tttcaatata tttcgcatca ccatcatcac cattattatt gacaaatatc cggaccagtt
     6361 tgaactctgg cacgaacaca ttctgaaata tgcgttttat ggccttatat tcctgatatg
     6421 ggcactgtgg aatgaaaagt ttgtagaaaa aaagccgttg agttagcttt cacctgccat
     6481 taggtttcta tccggcgagt tttccccttt tcaggtgtaa tcagaatatc tttgcttttt
     6541 cgccgttgat tacccggagc ccgttggccg ccagagcagt catttcgtct tcacccggat
     6601 acacaaatac agcagcataa ggttcaatca tttctttaat atcacgcact acgtaatcat
     6661 taaaagccac tccacccgtc agaatgacgc cgtctgcctt tcctttgagc actgcgagca
     6721 tggctcctat ttcttttccg atggtgtatg acatggcgtg cagcattgag gcaacgtgcg
     6781 tgtcacctcc ttctgctttt ttcaccagct cctgcacatc gttggagcct acgtgagcta
     6841 tgagtccgcc tttcccggcg agttttcgga gcaattcgtt ttcagtgtat ttgcccgaga
     6901 aacacaatct gaccaaccgg ccattatcca gagtacctgc acgttcaggt gaaatgggtc
     6961 cgtaaccatc cagtccctgg ttcacatcca ctaccctgcc ctgccggtga accgctacgg
     7021 taacacctcc gcccagatgt gcaatgacca ggttgagttc ttcgtatttt ttacccacct
     7081 tggcagcatg gtatcttccg gctgctttat gattaagtgc gtggaaaatg gactgtcggg
     7141 gtatttcggg aagtccgctt acacgagcta catcatccag ttcatctacc gccaccggat
     7201 cggcaatgta ggccttgcat cccggaatct gacgggcaat tttatctgcc agaaatgcac
     7261 ccagattact cgcatgctcg ccacgttcgg ctttgcgaag cgctgcgagc atccgctcat
     7321 tcacttcata cactcccgac tgtaccggag gcagcatacc accacgaccc atgattatag
     7381 aaaaatcgag cgctaatccc ttttctctca acgaattcat tatcaactca tacctgaagt
     7441 caaactgatc gatcacatga ggaaattcca tcagtttttc gacagaatga cgcaccgaaa
     7501 aaagttcagt ctgcttatcg tcttcgtaga cagccacttt ggtggaagta gagcccggat
     7561 tgatcacgag aattttatgc atattggatg tattttattt gacaaagata cacgattttg
     7621 ttaaaatacg gatattttca gggatttttt tgacattttg tcattaattg tatggcaaat
     7681 cattttctta taacctgtaa aaaaacatcc cgaaaaaatt gaaacccctg acggattagc
     7741 tatgttattt taatgaaaaa actgttaatg cattcatttt acctttcatt tttttatata
     7801 tttgtaaatc aacagattat aaaaaaaatc aacatttggt cttttttttc atcgaacgct
     7861 cagcacaaaa tactgttttt caaccactta tccgattttt ttttattcaa gtggaacaga
     7921 agttttttcg atccgttttt tttatcaatt ttgaaaactt aatgtacata aacctttccc
     7981 catgccaaat tcattaaaaa cctacacgct cagcaactat aaattaatac ctcaaatatc
     8041 cggcctgtcg gatgagatga ttgaagccat agaggtagtt ggacgcgtat tgccgttcaa
     8101 aacaaacagt tacgtggtaa acgagctgat tgactggaca caaattgaaa ccgatccgat
     8161 gtttaccctc acattcccga ggcgcgagat gcttgaaaca agacactacc agacggtaaa
     8221 aaaactactg gatgaaaata ctgataaggc tttactggac agaaaaatac acaaaatacg
     8281 gatggaactg aatccaaatc ccgcgggaca ggaacataac gtgcccgaaa tgaacagtat
     8341 caaactcaaa ggcatccagc ataaatatcc cgaaaccgtt ttgttttttc cgagtcaggg
     8401 tcagacctgc cacgcgtatt gttcgttttg ttttcgctgg ccgcagtttt cgggcatgag
     8461 cggattcagg tttgcaatga aagaagcaga acttctgaag caatacctga ttaaaaacaa
     8521 aaaagtgacc gacctgcttt tcaccggcgg cgacccgatg acgatgagtt cacggttact
     8581 tgcaggatat atcgagccgc ttcttgagcc cgaatttgat catatccgct ccatccgaat
     8641 cggcacaaag tcgctttcgt attggcctta tcgctttctg aaagatgatg acagcgatga
     8701 tatcatccga ttgtttgaaa aaattattca atcgggaaaa aatctttcca ttcaagctca
     8761 tttcaatcat cccgtagaac tttcgaccga tgcagtcaga aaagccattt cgcgtatccg
     8821 cagcaccggc gctcagatca gaactcagtc gccgctgctc aggcacatca acgacaagcc
     8881 ggaactgtgg gcagaaatgt ggcgcaagca ggtggactta agctgcattc cttactacat
     8941 gttcatagcc agagataccg gttcgaaacg atatttcgaa cttccactgg agaaatgctg
     9001 ggatattttc cgcaaagcat acaggcaggt aagcggactt tgccgcacgg ttcgtggtcc
     9061 gagcatgagt tgctctcccg gtaaagtgca ggtgctcggc gtgcaagaag tgatgggcga
     9121 aaaagccttt gtgttgcggt ttttacaggg ccggaaaccc gaatgggtgg atgtaccgtt
     9181 ttttgccgca tacgatccaa aagctacctg gtttgacgaa cttgagccgg catttggaga
     9241 aaaaaaattc ttttttgaga aaaacgaagc tacaaaaccg agagatcctg aattcctttt
     9301 tgaatagttc tgttttgatt tttttatact gaaaaaaccg atacgaaatt tgtaaattca
     9361 tatcggtttt ctgatttcag ccttcacccg atgagccgaa ttcatgcatc agcttatttg
     9421 ccggaaacat cagacggtga atttttcaaa aataatatct aatcaaaata aacttttgca
     9481 ttcatgtttt ttacccgtga ttttgtccag tttgtcacct tttcgcggtc ggatggactc
     9541 agattacgtt ttacattgaa aattacaatc ggagttttca ccgtatccgg atgaatgtat
     9601 gtaactgagt atgagcattt agtgatttgc ggataaaatt cccttacttc agccagaatt
     9661 tcttttccct tattttgtat attttccagg ctatccagtc tgttttgagt ttgccggagc
     9721 gagaatccgg tagaagtcag ctgattattg agtgtttcta cttctgtaag tcttctttgg
     9781 gtttccgaat gcagttgact ttgatctgta ccgctcagaa tttctatttt agccgtgtcc
     9841 agatttaatt ccagtgctgt ttttttaata tcggttttgg tctggttcga aacaccgtat
     9901 cccgaataga tcaggcgtat ggacttgttc gaaggattga tgttactatc aaaaagataa
     9961 ctgttttcct gtacagaaac agttgcgatg agattcctgg ctttatcagc aaaatcttca
    10021 ttttgaacga gtttataacc gaaataaaca cttggaatag ctgtaacaag cagtatgaaa
    10081 gtaatgtaat gatttacccg ccgggctctt tctttatcca caatggttcg gatagggaat
    10141 ttgagtacct gcgacacgaa aagtgcagct acagcaatga aaaccgtatt gatggtgaat
    10201 aaataaagtg caccgaaaaa gaaactgaac tgaaaagtgg cgagtccgta accggcagta
    10261 caaataggag gcattagcgc tgtggctatg gctacgcccg gaattacatt gcccttcagt
    10321 ttgctgctca atgccagcat accggccaca ccgccaaaaa gggcaataaa aacatcgtaa
    10381 atggtaggag aagtgcgtgc aagcaattcg gaatgggcag tatttaccgg cgaaatgaaa
    10441 aaataaaagg ccgaagtgat gaggctgaca accactgcaa atgtaaaatt tttaatggct
    10501 ttacggaaaa gcaaaaaatc atacgttgcc agactgtagc ccattccgtt gataggaccc
    10561 atgagtggcg aaataagcat ggcaccaatt atcaccgccg tggaattcat gttgagcccc
    10621 actgaagcca ctaatatagc acatatcagt acccaaaggt ttgtgccctt aaaaacaact
    10681 cctttttcga cttcggaatg aatcgtttca aaatcctccc tttcgccggc tatattgaaa
    10741 tagctcaaat atctgaatat cttatttttc atggtatagc ctgtaaatat cgacaaagat
    10801 aataaattaa taaacagaaa ttgttatcaa ctcattgtac aacaaaaaac agattgagaa
    10861 aaaaaatcat ttttctgctt tcgactttga atcgatattg aaagtcagta ttgtatgttt
    10921 ttttcctgtc ttttattttc cgggtctcca gcgatagaaa atgctggctg ttttcggaaa
    10981 aatgccgttt tgagcagtac gcacatcttc ggtgacgtag aatgcgtttt catcaaattc
    11041 tttaatcaat ttatctattt ttttcatcag ctgacgtgcc actacggttt caatcacatc
    11101 taccttaccg accgaaccct cgccccttac caaagttgct ccgaatccat ttgcattgag
    11161 tagttgaact aaagtggaac cgttttcttt ggtataaacc cggtaaagca acacaccaaa
    11221 agccagctta ttttcaatta aaatgcccac ataagtacct gtagcataac cacctgcata
    11281 agaaagataa gctatgaggt cacttgcccg ggagagaatt tgtgatatga tcacaatcca
    11341 gatgaacact tcgaaaaaac cgatgacagg tgcaatccct ttttttcctt tggaaacaaa
    11401 aataatgcga agtgtaccaa gcgtcacgtc acaaatacgc ccgaaaaaaa tcataacggg
    11461 cagtaaataa ggatattgat ctaaaaaatc gaacataaga tgataaatgt tagttttgag
    11521 ttctgagttc tgagttttga gttctgagtt ctgaattctg ctaaaagtta tttctaaaac
    11581 caacctttta aacaaaccat tcgtccggaa agtttaccag ataaacctga tcggtagcgc
    11641 gggtcaaagc tgtgtaaagc caccggaaat attcggcatt tatctgatcg tcgggcagga
    11701 atccgtggtc aataaatact tttttccact gtccgccctg tgctttatga caggtaactg
    11761 cataggcaaa tttcacctgc agggcgttga aaaactcgtt ttcaagcact tttcgcacca
    11821 gctgccttct gttggttatt tccggataat cttcggctac ggccgtaaaa agccggtttg
    11881 tcatctcatt catctgtgcc ggattttcag ccgtcaatcc atccagccat acgcgggcat
    11941 caatttccca tccgtaatca agcgaacgca gcgagagatc tgcaaaccga aagccgtaca
    12001 tttcacggtg tttccgtatg cgggtaactt ccagcacatc gccgttggct ataaaatcta
    12061 tttcgggata gggtttgttc cagaaataat tattccgtgt taccatcagc aaatcgccgt
    12121 ttgagagttg ctcttcgcgc atcatcaccc gcgagcggat accatcgttg tagaggtttg
    12181 cccgcttgtt ggagcgggtt atcacaatgg tatcctccac acccaccccg ctgtacgaac
    12241 gctggatggt atcaatcagt tccatcccgg caagccgctg aatatctgta aatgtgttta
    12301 atttgaattt cggatgctcg ccggtagttt cctgtgccag atgcatcctc agattggttg
    12361 catgaaacaa aatgccactt tccagtgcct gacgaaccac ataagtgagt tcaaactcat
    12421 ccacatgcaa tccgaatgat ttcagcgcct cggtttcgag tgccggactt tgtgtctgct
    12481 ccaccggcgg gagctgtgcg gtatcaccca gcaacaacag actgcaacca tcgccactgt
    12541 acacgtattc gatcagatca tccagcagcc ggccactgcc gaaagatgca tccagacgag
    12601 tattggaaat catggaagcc tcatcgacga tgaaaagcgt gtttttatga agattgtctg
    12661 acagttgaaa ccgaaaatca gtaatggatt tctggcggta gattttcttg tggatggtat
    12721 aagccggaaa tccggaatag gccgaaagca ctttggctgc tcgaccggtg ggtgcaagga
    12781 gtactgtttt ttgttgcaaa gcattcatag cacgcaccag cgcacttaca atgctcgttt
    12841 ttcccgttcc tgcatatcct ttcagcagga aagccttttc ggaatcggcg gacatgataa
    12901 aagcccccag cagatctatc aaaactgact gctgtccggt cggttcgaaa ccaaattcct
    12961 ctctgatttt cctgctgata aagtcgctta acatgcttgc aaaggtagag aatatttatt
    13021 gcatatttag ctaaagaaaa attcaattcc tatgaaaaca aatttgtaaa agtattgggc
    13081 tgtttcagat gattatcgca attatttatt atttaacgtg agttcgaaat aatttttgtt
    13141 cgtaggacat ctatttcaat agaatgaatt tatcggttat atttttcccg cagggaatat
    13201 atcccctacg gggaattagg tcctgtttaa tatataactc gtctcgactt ttaaaacata
    13261 gagatcaata gtcttatttt attgaatttt cgtgctgttt tatttaattt cgataacaat
//
LOCUS       DAORKP010000052        13191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_428_length_13191_cov_8.720691, whole genome shotgun sequence.
ACCESSION   DAORKP010000052 DAORKP010000000
VERSION     DAORKP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_13191_cov_8.720691"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLN75_04405"
     CDS             complement(<1..353)
                     /locus_tag="PLN75_04405"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRG03393.1"
                     /translation="MEYKLKCIGILTSGGDAPGMNAAIRSVTRAAIFNGIRVKAIYRG
                     YKGLITGEIKEFQTQNVSNIIQQGGTILKTARCDEFKTPEGRKLAYENMKKEGIDALV
                     VIGGDGTFTGARVFA"
     gene            complement(409..1278)
                     /locus_tag="PLN75_04410"
     CDS             complement(409..1278)
                     /locus_tag="PLN75_04410"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRG03394.1"
                     /translation="MVNIEIDPKSGFCFGVVNAIKKAEEELAKGTPVYCLGDIVHNGQ
                     EVKRLTEMGLVTINLEEFKNLRDVKVLFRAHGEPPSTYEIARQNNIELIDASCPVVLK
                     LQKRIKNSYEAATEEQQIVIYGKPGHAEIVGLLGQVDNNAIVIENKDDLSKIDFTRNV
                     RLFSQTTKSLAGFNHLVATISERMAPNAVFEYSDTICRQVANRIPNIAIFAEKNDIIL
                     FVSGKKSSNGKVLYEHSKSINPNTYLINDPEEIDGLNLDLTKKIGICGATSTPGWLME
                     AVAERIRQKATNK"
     gene            complement(1522..2205)
                     /gene="cmk"
                     /locus_tag="PLN75_04415"
     CDS             complement(1522..2205)
                     /gene="cmk"
                     /locus_tag="PLN75_04415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRG03395.1"
                     /translation="MKKIIVAIDGFSSCGKSTMAKELARYAGYIYVDTGAMYRAVALF
                     ALRNGWMNDEEISETELKNHISDIKITFKTNESGLQETFLNGENVEKQIRTLEVANGA
                     SRISAIGFVRREMVRQQQAMGIEKGIVMDGRDIGTVVFPDADLKIYLTASPEVRAQRR
                     YDELKAKGENVDFEEVLANVRERDFRDTNRAESPLYKAEDAIEIDNSHLTLQEQRDLL
                     KTLFHQKTF"
     gene            complement(2424..3461)
                     /gene="porQ"
                     /locus_tag="PLN75_04420"
     CDS             complement(2424..3461)
                     /gene="porQ"
                     /locus_tag="PLN75_04420"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRG03396.1"
                     /translation="MLKRLVLFFLFIFNLISLFPQTGRGVYRFLDLPVSSRMAAIGGM
                     NVSLRDNDVNFAFMNPALLTGETHGVIGLNMANYLADIKFGSAVYGHNFGENNFTSFG
                     VQYVDLGKFEGYDELGNFTEDFTAKDIALYMSYARRLDDYFTVGATFKPIFSSYERYT
                     SFGIAFDAGINYSRDLFSAGLVFRNMGAQLKAYYPDEQGQHREPLPFDIQLGVSKKFA
                     HAPIRISGTLNNLNRWNVAGYQTDKTTTNLDGTTEEKKVKPVDMAFRHVVLGVELVPS
                     DNFYIALGYNHRRNQEMTMEGFKSLAGFSAGAGLKLYKFHVGFGLTQFQVGNFAYQFS
                     FSTDLNDFVSF"
     gene            3713..4969
                     /gene="hflX"
                     /locus_tag="PLN75_04425"
     CDS             3713..4969
                     /gene="hflX"
                     /locus_tag="PLN75_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRG03397.1"
                     /translation="MPNRNEIKTEIYQETAILVGLITADQPEDKAREYLDELAFLAET
                     AGAIPEKMFLQRLEYPHPNTFVGPGKLQEIKAYVEENNIGIVIFDDELSPRQVRNIEK
                     ELVDQSPNAVDVVKVLDRTNLILDIFARRAQTANAKTQVELAQYQYILPRLTGMWTHL
                     ERQRGGGIGRYGPGETQLETDRRIILKKISLLKEDLKVIDRQMATQRKNRGKMVRVAL
                     VGYTNVGKSTLMNLLSKSEVFAENKLFATLDTTVRKVIIENLPFLLSDTVGFIRKLPH
                     HLIESFKSTLDEVREADLLLHIVDISHPNFEEQLEVVNQTLKEIDKSEKPMILIFNKI
                     DAFTYTPKESDDLSPVKRENISLEELENTWMSKLHENCIFISAKNKTNLEELKKMVYE
                     KVKIIHVERYPYNDFLFDKYNEEEMS"
     gene            5001..6986
                     /locus_tag="PLN75_04430"
     CDS             5001..6986
                     /locus_tag="PLN75_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRG03398.1"
                     /translation="MDNYRNLTEKEIATLVVYGCNADNWNNITVAQDFNPAYISNVSF
                     SGNVRLGTFNKVFEMEGGFKKHSGIYNCCIHNCTIGNDVFIDKIQNYIANYHIGNGCY
                     IENTNTMLVDQNSSFGNGTRVGVMNETGGREIPVFDHLSAPLAYILTLYRHRPEVIEK
                     LQNMIDNYARQISSEMGEIGENSIIVNCGRIKNVKIGEYASIKGASNLKNGSVNSNKK
                     APVAIGSGVKATDFILSSGTSITDSTLISKCFIGQGCIMDKHYSALDSVFFSNCQGMH
                     GEATAIFAGPYTVSHHKSSLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGIVERGSKT
                     TSDSYLLWPAKIGTFTLVMGRHTSHSDTTDLPFSYLIENATESYLVPAVNLRSVGTIR
                     DAQKWPKRDNRKDDVKLDPINFNLLSPYTIHKMYKGVEVLENLKKISGETTELYTYQN
                     CRIRNSSLNKGIKLYKTGIYKFLGNSLISKIVKENPSSFAELQKSLASATRKGSGDWI
                     DLSGLIVPKKEVERLLNDIEKDKMTLKSIQKRFEEMHTEYYNYEWTWAQEKLEEYWKK
                     PIAAVTKEEINSFIRLWRDSVVELDNLIYDDAKKEFNLISMIGFGVDGDDEQKHKDFE
                     NVRGSFEMNPFVKEVLNHIERKTALGNKTIEFVENLV"
     gene            7059..8234
                     /locus_tag="PLN75_04435"
     CDS             7059..8234
                     /locus_tag="PLN75_04435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRG03399.1"
                     /translation="MNNFSFYSPTYFEFGRNTEEKAGKLISRFGGKKVLIHYGSGSVV
                     RSGLLGRVIASLDAEGIHHIELGGAVPNPRSGLVYEGIELCRKEKVDFILAVGGGSAI
                     DSAKAIAIGVPYEGDFWDFYTGRKIEEALPVATILTLSAAGSEASASSVITHENGMVK
                     RGTLSDKIRPVFSILNPELTTTLPPFQTAAGATDMFAHVLERYFTNTRDVEITDRLCE
                     AVMLTIINEAPKAITNPADYEARANIMWAGMVAHNDICGVGRVQDWGSHQLEHELSAL
                     YDVAHGAGLAVMFPAWMKFTMKHDVMRFAQFAVRVFGCQMDFQQPERTAREGIERFES
                     FLKAIGMPVRFKELGAKREDIPTLIQMLKMDKRGVGNFVKLSAEDVEAIYNLAADEN"
     gene            8571..9128
                     /locus_tag="PLN75_04440"
     CDS             8571..9128
                     /locus_tag="PLN75_04440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRG03400.1"
                     /translation="MKNFKVGVVVGSLRKESFNKKAAKALVENAPENLRFEFLEIGNL
                     ELYSEDLDLTPPQSWVDFRNKVSSMDAILFVTPEYNRSVPGVLKNALDVASRPWGQSV
                     WNAKPAAVVSVTIGSTGAFGANHHLRQTLAFLNMPVLAQPEAYIGNAMSLFDENGKLT
                     EDSTKDFFGRFMQAFAALIETNVAG"
     gene            9214..10428
                     /gene="aroA"
                     /locus_tag="PLN75_04445"
     CDS             9214..10428
                     /gene="aroA"
                     /locus_tag="PLN75_04445"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRG03401.1"
                     /translation="MESAINLALNPDKIQLEINLPASKSISNRALILNALSYSPFEIE
                     NLSDCDDTRVLINVLNSNDYRFDVGAAGTSMRFLTAFLAKTVGEWRITGSERMKNRPI
                     KLLVDALNSLGAKIEYLEKEGFPPLKIYGSALKGGHICLNGGVSSQYISALMMIAPYM
                     QQGLKISLEGEVISIPYIRMTINMMQDFGIKVEFQNNEINIKPQVYSPVPFQVESDWS
                     AASYWYEILSVAREGKITLRGLKKDSCQGDSRVAEIFESLGVSTEYTEHGVILTPDEK
                     LPVKHLTLDFKNIPDLAQTIAVTCCLKGITFDFTGLQSLKIKETNRIDALINELRKLG
                     YVIHEPAEGQLAWNGEKCLMNENPEISTYDDHRMAMAFAPAALLHPLTIRHPEVVSKS
                     YPGFWNDLRLIF"
     gene            10502..11689
                     /locus_tag="PLN75_04450"
     CDS             10502..11689
                     /locus_tag="PLN75_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRG03402.1"
                     /translation="MSFLSDLLAQYQEPQRVKKLGLYPYFRPIESDQDTVVKINGKPV
                     LMFGSNSYLGLTNHPKLKEGAIKAVEKYGTGCAGSRFLNGTLDIHLELEEKLAKLVHK
                     EAALVYPTGFTVNSGVVPCLVGRDDYLIYDERNHASIIEGKRLTFGKTLKYKHNDMEH
                     LEKVLRKCEPDKVKLIITDGVFSMEGDVARLPEMVALAKKYNASLYVDEAHSLGVFGA
                     TGAGVCEHFGVSDDVDLVMGTFSKSLGTIGGFIAADENIINYLKHTSRTLIFSASITP
                     ASTGCVLAALDVMAEETWRKDQLWANTRRAKEGFEKAGFEIGPTETPIIPLYVRDNMK
                     TFTLTRMLMDDGVFVNPVVTPAVSPEDTLIRYSLMATHTFEQIDESIEKISKVARHLG
                     IIE"
     gene            11879..>13191
                     /locus_tag="PLN75_04455"
     CDS             11879..>13191
                     /locus_tag="PLN75_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG03403.1"
                     /translation="MITVSNLAIQFGKRVLFSDVNLKFTPGNCYGVIGANGAGKSTLI
                     RMLSGDLDPTRGTIHFGPGERLSVLKQDHFEFDEFSVLDTVMMGHDKLWKVKEEKDAI
                     YLKEDFSDADGMRASELEEKFAEMNGWNAESDAAALLSGLGIKEDKHYLLMKELSGKE
                     KVKVLLARALFGKPDNLLLDEPTNDLDLETVMWLEDYLANYENTVLVVSHDRHFLDAV
                     STYTVDIDYGKISLFAGNYSFWYESSQLALEQQRNQNKKAEEKKKELEEFIRRFSANV
                     AKSRQATSRRKMLEKLNVDEIQPSTRRYPGIIFTPDREPGNMVLEVSGLGKTAEDGTV
                     LFKDVNFNVEKNDKIAFVSRDPRAMTAFFEIISGNDKDHEGTYNWGITITHAYLPLDN
                     SAFFNTELSISDWFAQFSSDTTESYIRSYLGKMLFSGEEIYKKASV"
BASE COUNT         4107 a         2791 c         2781 g         3512 t
ORIGIN      
        1 tgtgcaaata cgcgggcacc ggtgaaagtg ccatcgcctc caatgacaac aagcgcatca
       61 attccctcct ttttcatgtt ttcgtaagcc agtttacgtc cttcgggtgt tttgaattca
      121 tcacaacgtg ctgttttgag tattgtacct ccctgctgaa tgatattact tacattttgt
      181 gtctgaaatt ctttgatttc accggttatc agtcctttgt atccacggta tatggctttc
      241 actctgatac cgttgaaaat ggccgcccgg gttactgagc gtattgccgc attcataccc
      301 ggagcatcgc ctcctgatgt aagtattccg atacatttca gtttgtattc catattattt
      361 cttaattttt attacgttta tcaattgtcg taaatattga acaatgtttt atttgtttgt
      421 tgctttttgt cttatccttt cagccacagc ttccatgagc catcccggtg tggatgttgc
      481 tccgcaaatt ccgatttttt ttgtcaaatc caggttcaac ccgtcaatct cttccggatc
      541 atttatcagg taagtattcg gattaatgct tttgctgtgt tcgtaaagca cttttccgtt
      601 ggaacttttt ttgccgctca cgaaaagaat gatgtcattt ttctctgcaa agatagcaat
      661 atttgggatt ctgttggcaa cctgacggca aattgtatca gaatattcaa atacggcatt
      721 gggtgccatt cgttccgaaa tggttgccac aaggtggtta aaacctgcca gtgatttagt
      781 tgtctgtgaa aataaccgaa cattccgggt gaaatcaatc tttgataaat cgtctttgtt
      841 ttcgattaca atggcgttat tatcaacctg tccgagcaat ccgacgatct cggcatgacc
      901 gggttttccg tatatgacga tttgctgctc ttcggttgct gcctcgtagg agttttttat
      961 acgtttctgg agtttgagca ctacaggaca cgatgcatca atcagttcga tgttgttttg
     1021 cctggcaatt tcataggttg aaggtggctc gccgtgagct ctgaacagca cttttacgtc
     1081 acggaggttt ttgaattctt caagattaat ggtcaccaaa cccatttcgg tcaatctttt
     1141 cacctcctgt ccgttgtgaa ctatgtcgcc cagacagtac acgggagttc ctttggccag
     1201 ttcttcttcg gcttttttaa ttgcattgac cactccaaaa caaaaacctg attttggatc
     1261 tatttctata tttaccatgt ttaatttttt agtttatgtt taacgaggtt tttttttggg
     1321 aagtcgctgt gaatgatatt tttaattaca agaaaaaata aaaaatactt tcatttatga
     1381 tttgttttgt ttaaaatcat taatgaaatt ttgattttca cagggtagct tcgatttatt
     1441 aatgtattga ttacaaacag gataccacaa aaacgtacaa ttgtttctgt aaaataagac
     1501 tgtttttttc aaaaacttta tttaaaacgt tttttgatgg aataatgttt ttaacagatc
     1561 gcgctgttcc tggagtgtaa ggtgggaatt atcaatctcg atggcatctt cggctttgta
     1621 aagcggactt tctgcacggt tagtatctct gaaatcacgt tctctcacat tggcaagcac
     1681 ttcttcaaaa tctacgtttt caccttttgc tttcagttcg tcgtagcggc gctgagccct
     1741 cacctcgggc gatgccgtga ggtagatttt cagatcggca tccggaaata ctacggttcc
     1801 gatgtctctg ccatccatta caatgccttt ttcgattccc atggcttgct gttgacgaac
     1861 catttcgcgc ctcacaaatc cgattgcgct tatccggctg gctccgttgg ctacttcgag
     1921 ggtacgaatt tgtttctcca cattttcgcc gttcaggaag gtttcctgaa gtcctgattc
     1981 gttggttttg aaggtgattt taatatcgct tatgtgattt ttcaattccg tttcgcttat
     2041 ttcttcatcg ttcatccatc cgtttcgcag tgcaaaaagt gccacagctc tgtacatggc
     2101 tcctgtatct acataaatat agccggcata gcgagccagc tctttggcca tggtactttt
     2161 accacacgat gaaaatccgt cgatagctac aattattttt ttcattagag tttatatgga
     2221 ttaaaattat aatcacatag ttcacaatag attaatatca aaacagatag attttataat
     2281 ttgaaataca catagcagct tactataaaa tgtaagatat attgatgctt aagcatcaat
     2341 aactatgtgg tcgggtctta aataaattta taactatttg ttactaagtg tcttaaatat
     2401 tcaattgaaa taatgtctat ttcttaaaaa cttacaaaat cgttcaaatc ggtactgaat
     2461 gaaaactgat aggcaaaatt tccaacctga aattgagtga gtccaaaccc aacatgaaat
     2521 ttataaagtt tcagaccggc tcctgctgaa aatccggcca ggctcttgaa accttccatg
     2581 gtcatctcct gattgcggcg atggttgtaa cccaaagcaa tgtaaaagtt atcggatggc
     2641 acaagttcta cacccagcac cacatggcgg aaagccatat ccacgggttt aacctttttc
     2701 tcttcggtag ttccatccag gtttgtcgtt gtcttatcgg tctgatatcc cgctacattc
     2761 catctgttca gattgtttag cgtgcccgaa atacgaatag gcgcatgggc aaatttctta
     2821 ctcacaccaa gttgaatatc aaacggcagg ggttcgcgat gctgcccctg ctcgtcggga
     2881 taataagcct tgagctgagc acccatgttt ctgaatacca atcctgcact gaaaagatct
     2941 ctggaataat tgatgccagc atcgaaagct attccaaaac tggtgtaacg ttcgtatgac
     3001 gaaaaaatcg gtttaaaagt tgcccctacc gtgaaataat cgtcaagccg gcgtgcatag
     3061 ctcatataaa gcgcaatatc ttttgcagta aaatcctccg taaaatttcc gagttcatcg
     3121 tacccttcga atttccccag atccacgtac tgcaccccga atgaagtgaa attattttca
     3181 ccgaaattgt gtccgtaaac tgcacttccg aatttaatat cagccaggta attggccata
     3241 ttgagcccaa ttactccgtg agtttcgccg gtaagcaggg caggattcat aaatgcaaag
     3301 ttcacatcgt tatcgcgcag cgaaacattc ataccgccga tggcagccat acgcgatgag
     3361 acaggcaaat cgagaaaacg atatacacct cttcccgttt gcggaaaaag tgaaataaga
     3421 ttgaaaataa ataaaaaaaa caggactaat cttttcagca tgttcggact aaatgtggaa
     3481 cgaatgactc attacttaaa aaaaagatac aaagatatac ttttttggtg ttgaaatcca
     3541 attttctcaa cttatttata aacgaaaata ttctgaagta attataacag ttcaataaaa
     3601 aaataccaga accttccgag actattattt cgtcatttcg aaaaacagaa tagaattatt
     3661 ttaaaagaat tatcgtattt ttgcgggtca ggaattttta ttacaagtca taatgccaaa
     3721 cagaaacgaa ataaaaacag aaatatatca ggaaacagct atcctcgtgg gacttatcac
     3781 cgctgaccaa cccgaagaca aagcccggga atacctggat gaactggcgt ttctggctga
     3841 aacagccgga gccattcccg aaaaaatgtt tctgcaacgg ttggaatatc ctcatcccaa
     3901 tacattcgta gggccgggta aactgcagga aatcaaagcc tatgtggaag aaaacaatat
     3961 cggaatcgtc atatttgacg atgaactatc gccccggcag gttcggaata tcgaaaaaga
     4021 actggtggat caaagcccta atgccgtgga tgtggtgaaa gtgcttgacc gcaccaatct
     4081 gatactggat atttttgcca gacgtgcgca aactgccaat gcaaaaacac aggtagaact
     4141 tgctcaatat caatacattt tgccccggct tacgggtatg tggacacacc ttgaaaggca
     4201 gcggggaggc ggaatagggc gctacggacc cggagaaact cagcttgaaa ccgaccggcg
     4261 aattatactg aaaaaaatct ccctgttgaa agaagatctt aaagtcattg accgccagat
     4321 ggctacccag cgtaaaaaca gaggaaaaat ggttcgggtg gcattggttg gttatacgaa
     4381 cgtgggaaaa tccaccctga tgaatctgct cagtaaatcg gaagtttttg ccgaaaacaa
     4441 actttttgcc acgctggata caaccgttcg gaaggtaata attgaaaacc ttcctttcct
     4501 gctttcggac actgtgggat ttatccgaaa actgccccac catctgatcg agtcattcaa
     4561 atcaacgctc gacgaggtcc gggaggccga tttattgctc cacattgtgg atatttcaca
     4621 cccaaacttc gaggaacaac tggaagtggt aaatcagact ttgaaagaaa tcgacaaatc
     4681 cgaaaaacca atgattctca ttttcaataa aatagatgca tttacataca ctccgaaaga
     4741 aagtgatgac ctttcgccgg tgaaacgtga gaacataagt ctggaagaac tcgaaaatac
     4801 ctggatgagc aaactccacg aaaactgcat ttttatatcg gcaaaaaaca agaccaacct
     4861 cgaagaactg aaaaaaatgg tgtatgaaaa agtgaaaatc attcacgtag aacgttatcc
     4921 ctacaatgat tttctatttg acaaatacaa cgaagaagaa atgagttaat tttttaaaaa
     4981 acaacaacca aatcgcaatt atggacaatt acagaaacct tacagaaaaa gaaattgcca
     5041 ccctcgtggt gtacggctgc aatgccgaca actggaacaa tataaccgta gcacaggatt
     5101 tcaatccggc ttacatatca aacgtaagtt tttcgggcaa cgtacgctta ggcactttca
     5161 acaaagtatt cgaaatggaa ggcggcttta aaaaacacag cggaatttat aactgctgca
     5221 ttcacaactg caccatcgga aacgatgttt tcattgataa aattcagaac tatattgcca
     5281 attaccatat aggaaacgga tgctacatcg aaaacacaaa cactatgctg gtagatcaga
     5341 actccagttt cggaaacgga acgcgtgtag gggtgatgaa cgaaaccggc ggacgtgaaa
     5401 taccggtttt cgaccatctg tctgcaccgc ttgcctacat tctcaccctg tacagacacc
     5461 gtccggaagt gatagagaaa cttcagaaca tgatagacaa ttacgcccgg caaatcagtt
     5521 cagaaatggg cgaaataggc gaaaattcaa tcatagtcaa ttgcggtaga attaaaaacg
     5581 taaaaatagg agaatatgca tcaataaaag gtgcatccaa cctgaaaaac ggatccgtca
     5641 acagcaataa aaaagctcct gtagccatcg gatcgggtgt aaaagcaact gattttattc
     5701 tgagttcagg taccagtatt accgattcaa ccctgatttc caaatgtttc atcggacagg
     5761 gatgtattat ggacaagcat tattcagccc tcgattcggt ctttttctcc aactgccagg
     5821 gcatgcacgg tgaagctaca gccatttttg caggacctta cacggtttcg catcacaaat
     5881 cgagcctgct catagccggc atgttttcat ttctgaatgc cggcagcggc tccaaccaga
     5941 gcaaccacat gtacaaactc ggacccattc atcagggtat tgtagagcgg ggctccaaga
     6001 ccacaagcga ttcatattta ctgtggccag ccaaaatcgg aacatttaca ctcgtcatgg
     6061 gtcgccacac atcgcattcg gatactaccg atctgccttt ctcctacctg atcgaaaatg
     6121 ccaccgaaag ctatctggta ccggcagtca acctgcgaag cgtgggaaca atacgtgatg
     6181 cccaaaaatg gcccaaacgt gacaaccgga aggacgatgt gaaacttgat ccgataaatt
     6241 tcaacctgtt gagcccttac accattcata aaatgtataa aggcgtagaa gtgcttgaaa
     6301 atctgaaaaa gatttcgggt gaaactacag aactatacac ctatcagaat tgcagaatta
     6361 gaaattcgtc gctgaataaa gggataaagt tgtataaaac aggcatttac aaatttttgg
     6421 gcaactcgct cataagtaaa attgtgaaag aaaatccgtc gtcatttgcc gaacttcaga
     6481 aatcactcgc aagtgccacc cggaaaggtt cgggagactg gattgatcta tcgggtttga
     6541 tagtgccgaa aaaagaagtg gaaagactgc tgaacgacat cgaaaaagac aaaatgactc
     6601 taaaatccat acaaaaacga tttgaagaga tgcacactga atactacaac tacgaatgga
     6661 cctgggcgca ggaaaaactg gaagaatact ggaaaaaacc aatcgctgcg gtaaccaaag
     6721 aagagataaa ctcttttatc cgactctgga gagattcggt agtagagctc gacaatttga
     6781 tatacgacga tgcaaaaaaa gaattcaacc tcatatccat gatcggattc ggggtagatg
     6841 gcgacgatga gcaaaaacac aaggattttg aaaacgtaag gggaagtttt gaaatgaatc
     6901 cctttgtaaa agaagtactt aaccacatcg aacgtaaaac ggcattaggc aataaaacca
     6961 tcgaattcgt ggaaaatctt gtgtaattgt gccaaaggaa tagtaataat taaattctca
     7021 aataaaattt ctaatcatta tactaaaaca acaatcagat gaataatttt agcttttaca
     7081 gccctactta ttttgaattc gggcgaaaca cagaagaaaa agccggcaaa cttatcagca
     7141 gattcggagg aaaaaaagta ctgatacact acggaagcgg ttcggtagta cggagcgggc
     7201 ttttgggcag agttattgca tcactggatg cagaaggtat tcaccacatc gaacttggtg
     7261 gagcagtgcc caatccgcga agcggactgg tatatgaagg cattgaactc tgccggaaag
     7321 aaaaagtgga tttcattctg gccgttggcg gcggaagcgc tatcgactca gccaaagcca
     7381 tcgccatagg agtaccctac gaaggcgatt tctgggattt ctacaccggc agaaaaattg
     7441 aagaagcatt acccgtagcc acaattctta cactctcggc tgccggaagc gaagcatctg
     7501 cctcatcggt catcactcat gaaaacggaa tggtaaaacg gggtaccctg agcgacaaaa
     7561 tccgaccggt tttctctatc ctgaatccgg aacttaccac cactcttccg ccgtttcaga
     7621 ccgctgcagg tgctaccgat atgtttgcac acgtactcga aaggtatttt accaacacca
     7681 gggatgtgga aattaccgat cggctttgcg aagcagtcat gctcactatc atcaacgaag
     7741 cacccaaagc aataaccaat cctgccgatt atgaagcccg cgccaacatc atgtgggcgg
     7801 gcatggtggc tcacaacgat atctgcggcg taggacgcgt tcaggactgg ggttcacatc
     7861 agctcgaaca cgagttatca gctctctacg atgtggcaca cggagcagga ctggctgtaa
     7921 tgtttcccgc ctggatgaaa ttcaccatga agcatgatgt gatgcggttt gcacagttcg
     7981 cagtacgcgt tttcggatgt cagatggatt tccagcagcc tgaacgaaca gcccgggaag
     8041 gaattgaacg atttgaatcg tttcttaaag ccatcggaat gcccgtaaga ttcaaagaac
     8101 tcggagcaaa gcgcgaagac atccctacat taattcaaat gctcaaaatg gacaaacggg
     8161 gtgttgggaa ttttgtaaaa ctctctgcag aagacgttga agcaatctac aacctggctg
     8221 ctgatgaaaa ttaaaaaaga aaaataagcc ccgtaaacct gcctgcggca gggaggtacc
     8281 ttcttggggg actttcagag ctttataata tggtttttct actaattaag gatttttttc
     8341 gatatagtga attgaaaaaa atctaaattt tgctcccaaa aaaaactata aattaaaatc
     8401 tcagataact ctgctaaaat taaaacgtta gcagagtttt ttgttgatta gacagtagga
     8461 ttataaaaat catctataca gaataaacaa aataaattca aacaattttt tcaattgatt
     8521 tgttataaag agcaaaatca aaaattacaa acaaaaaaca atacaaaaaa atgaaaaatt
     8581 ttaaagtagg ggttgttgta ggcagcttgc gcaaagaatc attcaataaa aaagcagcaa
     8641 aagctttagt agaaaatgca cccgaaaatt tgagattcga attcctcgaa atcggaaatc
     8701 tcgaacttta cagcgaagac ctggatttaa ctcctccaca gtcttgggtt gatttccgaa
     8761 ataaagtgag cagcatggat gccatacttt ttgtaactcc ggaatacaac cgatccgtac
     8821 cgggtgtact taaaaatgca ctggatgtag catcgcgtcc gtggggacaa agcgtatgga
     8881 acgccaaacc ggctgccgta gtgagcgtaa caatcggtag tacgggcgct ttcggagcca
     8941 accatcactt acgtcagaca ctggctttcc tcaatatgcc ggtactggct cagcccgaag
     9001 cttacattgg caatgcaatg agtttatttg acgaaaacgg aaaactgacc gaagatagca
     9061 ccaaagattt cttcggaaga tttatgcagg cattcgccgc gctgatagaa accaacgttg
     9121 cgggttaaat tttcgtaatc cattcaaaat aactactttt gacttcctgt ttccgaaaaa
     9181 tggaacagga agttttcttt ttaaaacaaa aacatggaat cagctatcaa tcttgcactt
     9241 aacccggata aaattcagct ggagataaac cttccggctt ccaaaagcat cagcaacaga
     9301 gcgttgatat taaatgcact ctcttacagt cccttcgaaa tcgaaaatct ttcggactgt
     9361 gacgacaccc gtgtgctgat caacgtgctg aacagcaacg attaccggtt tgatgttggc
     9421 gcagccggca cgtccatgcg gtttctcaca gcatttctgg ccaaaacggt gggcgaatgg
     9481 cgcatcaccg gcagcgaacg aatgaaaaac cgtcccataa aactgcttgt agatgcactg
     9541 aactcgttgg gagctaaaat tgaatacctt gaaaaagaag gatttcctcc gctgaaaatt
     9601 tacggcagcg ctctcaaagg cggccacatc tgtctgaacg gaggcgtaag cagccagtac
     9661 atttcggcac tgatgatgat agcaccctac atgcagcaag gattgaaaat aagccttgaa
     9721 ggcgaggtga tttcaatacc atacatccga atgaccatta atatgatgca ggattttggt
     9781 ataaaagttg aatttcagaa caacgaaata aacataaaac cgcaagtgta ctctcctgtt
     9841 ccgtttcagg tggaatccga ctggtcggca gcttcgtact ggtatgagat actctcggta
     9901 gcccgggaag gaaaaatcac gctgcgcgga ttgaaaaaag acagctgtca gggcgacagc
     9961 cgggtggctg agatttttga atcgctgggt gtttcgaccg aatacaccga acacggagtg
    10021 atactgactc ccgacgaaaa attaccggtt aagcacctga cgctcgactt caaaaacatc
    10081 cccgatctgg cgcaaactat agcagtaacc tgttgtctga agggaataac gtttgatttc
    10141 acagggttac aaagtctgaa aattaaggaa acaaaccgca ttgatgcatt gataaacgaa
    10201 ttgagaaaat tgggttacgt aattcacgaa ccggccgaag gtcagcttgc atggaatggc
    10261 gaaaaatgtt taatgaatga aaatcccgaa atttccacct acgacgatca ccgcatggcc
    10321 atggcttttg cgcccgctgc actgctgcat ccgctaacta tccgccatcc ggaggttgtc
    10381 agcaaatcat atcccggttt ctggaatgat ttacgtctga tattttaaga aatttcaaaa
    10441 aatccaattt ttaaataaaa tattgtatct ttgcacccta aaaaaatttt aaatacaact
    10501 tatgagtttt ttaagcgatc tattagctca atatcaggag cctcaaagag taaaaaaatt
    10561 gggtctttac ccttactttc gtccaatcga atccgatcag gacacagttg taaagataaa
    10621 cggaaagccc gttctgatgt ttggttcaaa cagctacctc ggacttacca accatccgaa
    10681 acttaaagaa ggtgcaataa aagcagtaga aaaatacggt acaggatgtg ccggctcacg
    10741 atttctgaat ggaacactgg atattcacct cgaactggaa gaaaaacttg caaaattagt
    10801 tcacaaggaa gcagcattgg tttaccccac cggttttacc gtaaattcag gagttgtacc
    10861 gtgcctggta ggacgtgacg actatttgat ttacgatgaa cgcaatcacg cttccatcat
    10921 cgaaggaaaa agactcacat tcggcaaaac actgaaatac aagcacaacg acatggagca
    10981 cctggaaaaa gtgctcagga aatgtgaacc tgacaaagta aaacttatca tcaccgacgg
    11041 agttttcagt atggaaggag acgtagccag actgcccgaa atggtagcat tagccaaaaa
    11101 atacaacgct tcactttatg ttgacgaagc tcactcgctc ggtgtattcg gagccacagg
    11161 agcaggagta tgcgaacatt tcggagtttc agacgacgtg gatctggtga tgggaacatt
    11221 cagcaaatcg cttggaacaa tcggaggatt tatcgctgcc gacgaaaata ttatcaatta
    11281 tctgaaacat acttcgcgaa ccctgatatt cagtgcttca atcactcctg cttcaaccgg
    11341 atgtgtactg gcggcacttg atgtgatggc cgaagaaaca tggagaaaag atcagctctg
    11401 ggcaaatacc aggcgtgcca aagaaggatt tgaaaaagca ggtttcgaaa tcggtccgac
    11461 cgaaactcct atcattccgc tttacgtgcg tgacaacatg aagactttca cgctcacacg
    11521 tatgctcatg gacgacggcg tatttgtcaa tccggtggtt acacctgcgg taagccccga
    11581 agatacgctg atacgctatt cgctcatggc aacacacaca tttgaacaaa tcgatgaatc
    11641 catcgaaaaa atctcaaaag tagcacgcca tttaggtata attgaataaa aataaaacct
    11701 aatctgcccc taaccccaca cctgaggcgg gtaaatcctg atgggggaat aagtgttggt
    11761 aagtcagaat tagactaaca taaaaacaaa tattattttt ttttaattag ataatgaatg
    11821 acaggttcac aaatccccca tcagggggtt agggaacagg tcattctctt ttttgcttat
    11881 gataactgtt tcgaatcttg ccattcaatt tggcaaacgt gtacttttct cggatgttaa
    11941 tctcaaattc actcccggca actgctacgg cgtaatcggt gccaatggtg ccggcaaatc
    12001 cactctcatt cgcatgctga gcggtgatct cgatcctacg cgcggtacca ttcattttgg
    12061 acccggcgaa cgactttcag tgttaaaaca ggaccacttt gagtttgatg aattttcggt
    12121 tcttgacacc gttatgatgg gtcacgacaa gctttggaaa gtaaaagaag aaaaggatgc
    12181 catatatcta aaagaagatt tttcggatgc agacggcatg cgtgcttctg aactggaaga
    12241 aaaatttgct gaaatgaacg gttggaatgc tgaaagtgat gcagctgcgt tgttaagcgg
    12301 acttggaatc aaagaagaca agcattatct gctgatgaaa gagctgagcg gaaaggaaaa
    12361 agtgaaagtg ctgctggctc gtgctttatt cggaaaaccg gacaatctgc tgctggatga
    12421 gccgaccaac gaccttgacc tggaaaccgt aatgtggctg gaagattatc tggcgaatta
    12481 tgaaaatacg gttcttgtgg tatcccacga ccgccatttt cttgatgcag taagtacata
    12541 taccgtggat attgactacg gaaaaatctc actgtttgcc ggaaattatt cattctggta
    12601 tgaatccagt cagctggcgc ttgagcagca acgcaaccaa aacaaaaaag ccgaagagaa
    12661 aaagaaagaa ttggaggaat ttatccgccg atttagtgct aatgttgcta agtcgcgtca
    12721 ggcaacaagc cgtcgcaaga tgcttgagaa actgaatgtg gatgaaattc agccttccac
    12781 acgtcgttat ccgggaatta tttttacgcc agatcgcgag ccgggaaata tggtactcga
    12841 agtttcggga ttaggtaaaa cggctgaaga tggcactgtg ctgttcaaag acgtgaattt
    12901 caatgtagag aaaaatgata aaattgcttt cgtgagccgt gatccgcgtg cgatgactgc
    12961 tttttttgaa ataatcagtg gaaatgacaa agatcacgaa ggcacgtaca attggggtat
    13021 aacgattacc cacgcttatc tgccattaga taattcggca ttttttaata cagaattaag
    13081 catttctgat tggtttgcac agttttcaag cgatacaacc gaatcgtaca tccgcagtta
    13141 tctgggaaag atgcttttca gcggggaaga aatttacaaa aaagcaagtg t
//
LOCUS       DAORKP010000053        13073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_436_length_13073_cov_9.538869, whole genome shotgun sequence.
ACCESSION   DAORKP010000053 DAORKP010000000
VERSION     DAORKP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_436_length_13073_cov_9.538869"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..435)
                     /locus_tag="PLN75_04460"
     CDS             complement(253..435)
                     /locus_tag="PLN75_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03404.1"
                     /translation="MKKVFLFFAVASIFVACNKPAQTETSTQADSLVVEEVTSAPADS
                     LNADSIAPVDSVAPAM"
     gene            complement(528..2255)
                     /locus_tag="PLN75_04465"
     CDS             complement(528..2255)
                     /locus_tag="PLN75_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG03405.1"
                     /translation="MIKENFIRLFEDSFRKNWDLNAYTNYGENLTLTYAQVAEEIARL
                     HLLFEQTEIKKDDKIALIGKNNANWAITYLATVTYGAVIIPILQDFNPNDVHHIINHS
                     ESKVLFVSDYIWENLEESNLRTVKAVYSLSDFRLVTLVNQPIATLLEESESKENIVAP
                     LNPGKLTKEHVNELFDKKYSDGFHKDSVRYIDKPNSELVCISYTSGTTGFSKGVMLTG
                     NNFAGNITFGIRTKLLAPGFKLVAFLPLAHAYGCAFDFLTATCTGSHVHFIGRTPSAK
                     ILLNAFAEVRPNVIFCVPLIIEKIYKKQIQPLLERPSMRWVLSIPFLDQTILAQIRKK
                     LIDAFGGNFSQVIVGGAPLNAEVEEFFNKIKFPFTVGYGMTECAPLISYSHTNDFLPK
                     SAGKVLDIMEAKIDRPDPKTGVGEIFVRGENVMKGYYKNEESTKEILDDEGWLHTGDL
                     GTIDANGNIFIRGRNKTMILSANGQNIYPEEIEAKLNNMPFVMESLVIDSKGKLMALV
                     CPDYEAVDAEGLSQEQLEKVMEENRKMLNSSVAAYESVAKIHLYPHEFEKTPKKSIKR
                     YLYTTAFES"
     gene            2502..4964
                     /gene="pheT"
                     /locus_tag="PLN75_04470"
     CDS             2502..4964
                     /gene="pheT"
                     /locus_tag="PLN75_04470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRG03406.1"
                     /translation="MNISYNWLKEYININVSPEKLAQILTSIGLEVGGVEEIQTVKGG
                     MEGLVIGEVITCSRHENSDHLSVTTVNVGTGEPLQIVCGAPNVAAGQKVVVATIGTKL
                     YSGDESFTIKKSKIRGVESHGMICAEDEIGLGTSHDGIMVLPADAKPGTLASEFFGIK
                     NDTLIEVDITPNRADAVSHYGVARDLAAYFTLHDETVKLTKPSIDGFKINNTVLSIPV
                     KIENTEACPRYSALTISGISVKESPDWLKQRLLAIGSRPINNVVDITNFILHELGQPL
                     HAFDADKITSGEVRVKNLPDGTPFTTLEGTERKLNPGDLMICNGDVPMCIGGVFGGFD
                     SGVTENTSKVFLESAYFNPVSIRKTARRHGLNTDASFRFERGCDPEMTIYALKRAAML
                     IQEVAGGIISSEIVDEYPNPVQPFEVTVKKNKIDNLIGKKIGRENILKILKGLEMEVT
                     ADTDDAYQLKVPAYRVDVQRDVDVIEDILRIYGYNNVEIGDNLVSTLSFVKKPDSHKL
                     QIIVSEQLTAQGFNEVLNNSLTKIGYYGKLTSYPENECVKIMNPLSSDLSVLRQTLLF
                     GGLENISRNTNHRNADLKLYEFGNCYQYLAGKKTDDQVLSAYNEDYHLGIWLTGNKYS
                     DSWINGSQKSSIYELKAYVENILVRLGFDLQKLKTAEYSSDIYTEAMSINSPNKKLLC
                     TFGIVSDAAKKTSDIEAEVFFADIYWTNLLQTLNTNDVKYKELPKFPEVRRDLAFLLD
                     TNITFAEIENTAFETERRILKKVSLFDVYEGKNLEPGKKSYAISFILQDENKTLTDSI
                     IDGTMKNIQKNIESKLNARLRS"
     gene            5112..6518
                     /locus_tag="PLN75_04475"
     CDS             5112..6518
                     /locus_tag="PLN75_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRG03407.1"
                     /translation="MSGFFGSISKNDCVDDVFYGTDYHSHLGTKRAGIAFFSGKNGFQ
                     RAIHSLEDGYFRNKFENDIKGFAGNCGIGVISDSEAQPMVVNSHFGRFGVATVSRIIN
                     INDLEEHFLKLHHTFSETSQGTVNPTELISMLIAEGNDFVSGIENVFEKVKGSCSMLI
                     LTENGIIAARDKLGRTPVVIGKKNNAYAAAFETCSFSNLGYETEKYLGPGEIVKLTAE
                     GWEQLRKPNVEMQICSFLWVYYGYPPSFYEGINVEETRYRCGAALAKNDPIEADFVAG
                     IPDSGVGHAVGYSHARGLPFKRPYTKYTPTWPRSFMPQSQHMRELVAKMKLLPNKSII
                     ENKKGIFLDDSIVRGTQLKDNVRDLHEAGIKEVNMRIACPPLTYPCEFLNFSRSRSSM
                     ELATHKAVKELEGTENVNMKEYSDAGSEKYAAMVDKIRENLGLTTLRFQKIEDLVEAI
                     GLPKEKLCTHCFDGSSHF"
     gene            complement(6584..6657)
                     /locus_tag="PLN75_04480"
     tRNA            complement(6584..6657)
                     /locus_tag="PLN75_04480"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6621..6623),aa:Asn,seq:gtt)
     gene            6748..7731
                     /locus_tag="PLN75_04485"
     CDS             6748..7731
                     /locus_tag="PLN75_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRG03408.1"
                     /translation="MKKVLGMGNALTDILFRIENDDILSKLNLVKGGMQLIENTQSEV
                     IKSHFTPGSATMATGGSASNTINGISKLGIESGFIGKISDDEIGRFFALNSLENGVTP
                     YMQKSKTPSGHCTILVSPDSERTLCTFLGAASELNAGDINQHFFEGYHFFHIEGYLVQ
                     DHDLIRTALKMAKDAGLITSIDLASFNVVNENLEFLHEIIEKYVDIIFANEEEAMAFT
                     GKKPAEAVRDMAQMCRIAVVKIGKDGSLIQSGTDFYDIEPHQVKSVDTTGAGDFYAAG
                     FLFGLANSYPLEVCGKIGSLVSSRVVEVVGTKMAKETWDEIAENVRLLTQN"
     gene            complement(7895..9271)
                     /locus_tag="PLN75_04490"
     CDS             complement(7895..9271)
                     /locus_tag="PLN75_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRG03409.1"
                     /translation="MELPFAESYKIKMVESIRRSTRHEREKWIRDAKYNLFGLKSGQV
                     FIDLLTDSGTGAMSDRQWSAMMLGDESYAGARSYFSLKNAISDLLGFDYFFPTHQGRA
                     AENLLFSAIVKEGDILPGNSHFDTTKAHIEYRKATALDCTIDEAKDTQLEIPFKGNID
                     LEKLENALKSYPKEKIPAVVLTVTNNTAGGQPVSMENIRETSRLCQKFDVLLLIDSAR
                     FAENAYFIKTREKGYSDKSIREIVREMFSYADVMTMSSKKDAVVNMGGFLAMKSEALW
                     KKCKIYCILNEGFITYGGMSGRDMEALAQGLEEGTDFDYLESRIKQVEYLGSRLDEFG
                     IPYQRPAGGHAIFLDAKKIISRVPAEEFIAQTLAIELYLEAGIRTVEIGALLADRDPV
                     TRQNRFPELELLRLAIPRRTYTNNHMDVVAVAVKNVFDRRNNIRRGYEIVSEEPIMRH
                     FTVELKPL"
     gene            9386..10306
                     /locus_tag="PLN75_04495"
     CDS             9386..10306
                     /locus_tag="PLN75_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRG03410.1"
                     /translation="MKINTGNVRIYIFITIAMLCWSFSFIWTKIALESFPPLTLITLR
                     LILASVLLYGFLKLSGRLQKIDKPDLKLFFALAFFEPFMYYVGETYGLTMVKPTLASV
                     IIATIPVFAPFFAFVFLKERFTIINMLGIGVSIIGVYLIVYNTDGSETTSVIGVALVF
                     LAVLSAIIYGLLLRKIPEKYSATNIIFYQSMIGLIYFIPVAAMVDLRLLARMVILRQS
                     VQALLMLTFFASVIAFVLFADVVRKIGITKSEVFTNLIPVFTALLSWAIFKEMMNSFQ
                     WIGVFVVILGVFVSQSKNKKAGRPKSEDVR"
     gene            10800..11516
                     /locus_tag="PLN75_04500"
     CDS             10800..11516
                     /locus_tag="PLN75_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRG03411.1"
                     /translation="MKLLYPLYFVYQWLIAMPLMLISTVITSILIFLISPVFPNHKIS
                     YLPGILWGKFVCYLFFIHVKISGMEHLDRKQSYVFAANHQSAFDILVIYGWLPYIFKW
                     LLKIELRKVPFLGAACQAAGHIFVDRSNPVAAKQSMQKAVEQLKNGVSVVIFPEGTRT
                     KTGKLGKFKRGAFLMALELQLPIVPITIAGSFERIKGWRTFPGTIKMTIHPPVDVTTY
                     GMDESSALLRNTQEIIRSGL"
     gene            11814..12416
                     /locus_tag="PLN75_04505"
     CDS             11814..12416
                     /locus_tag="PLN75_04505"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRG03412.1"
                     /translation="MSGKYDDLEKLRDLYSKGIITEAEFNAEKAKILNDNREAGVNET
                     TQTADNNNGYCSLMHLSQFSNFLFPGLGLIIPIVMWATRKNESKFADVNGKIIFNWKI
                     SSFIYTSVLVILVIAAALVFGTQIAFSGIGNYDTFQGMMDDNPMKIFSLIGALGIIII
                     PAIAIVVLDFIFTIIGAIKANKGEVWNYPLSIRFFSTKNQ"
     gene            12461..>13073
                     /locus_tag="PLN75_04510"
     CDS             12461..>13073
                     /locus_tag="PLN75_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG03413.1"
                     /translation="MIKLTDINKTYYTEATSLHVLKGIDLTIEKGEYVSVMGASGSGK
                     STLLNIIGILDNYDTGSYYLNDILIRDLSEKKAAWFRNEMIGFVFQSFNLINFKNAME
                     NVALPLYYKNVGRKKRNAIAMEYLDMMGLKDWATHMPSEMSGGQKQRVAIARAIISQP
                     KVLLADEPTGALDSKTSEEVMQVLKELNKTGITTVIVTHEQSVA"
BASE COUNT         3956 a         2623 c         2696 g         3798 t
ORIGIN      
        1 aaaataaaaa tacttttttc attttgtttt aatcctttaa aaatttaaca tgttatatat
       61 tttgttcaat tcaaaatcgg tgcaaagata tatcaatatt ttgatatatg attaataatt
      121 ttcatttttt tttatttttt ttttgctttt atctgtttta tccaaaaaaa gacttaaaaa
      181 caagtaaaaa aacatcaaaa aaatcagttc aatctgtttc tgataaaaca gtaaaaaaaa
      241 tcagcgagct gattacatag cgggagctac gctgtcaacc ggtgcaatgc tatcggcatt
      301 cagactatca gccggagcag aagtcacctc ttctactacc aaagaatcag cctgtgtact
      361 ggtttcggtt tgagcaggct tgttgcaagc cacaaaaatc gaagctacag cgaaaaataa
      421 aaatactttt ttcattttga gataattatt attaaaaaac ggtgcaaagg tacaacattt
      481 ttagtaatca tgtacaaata tttttttact tttttttaaa aaatttttta tgattcaaat
      541 gcagtggtat agaggtatct ttttatactc tttttgggtg ttttttcaaa ttcgtgcgga
      601 tagagatgaa tttttgctac actttcataa gcagcgactg acgaattcag cattttccga
      661 ttttcttcca tcactttctc aagttgttcc tggctcaaac cctcagcatc aacggcttca
      721 taatcaggac aaacgagcgc catcagtttt ccttttgaat caataaccaa actttccatc
      781 acaaaaggca tgttatttaa tttggcttca atttcttccg gatagatgtt ttgtccgttt
      841 gcgctcagta tcatggtttt atttcgtccg cgaataaata tattgccatt tgcgtcgatg
      901 gttcccaggt caccggtgtg cagccaaccc tcatcatcca gtatttcttt ggttgattct
      961 tcgtttttgt agtatccctt cattacgttt tcaccccgaa caaatatttc acccactccg
     1021 gtttttggat cgggtcgatc aatttttgct tccatgatat ccagcacttt tccggctgat
     1081 ttcggcagaa aatcgttggt atgcgaatag ctgataagcg gtgcacattc ggtcattccg
     1141 tatcctacgg tgaagggaaa cttaatttta ttgaaaaact cctctacctc agcattcaac
     1201 ggagcgcctc cgactattac ctgggagaaa ttgccaccga aagcatcgat cagttttttt
     1261 cttatctgtg ccaggatggt ctgatccaga aacgggatac tgagtaccca gcgcatcgat
     1321 ggtctttcga gcaggggttg aatttgcttt ttatatattt tttcgataat cagtggaaca
     1381 cagaaaatta cattcggacg cacttcggcg aaggcattga gaagtatttt ggctgatgga
     1441 gtgcgaccga taaaatggac atgactcccg gtacaggtag ctgtcagaaa atcaaaagca
     1501 caaccgtaag catgtgcaag cggcagaaat gcaaccagtt taaatcccgg cgccaggagt
     1561 ttggtacgaa tgccaaaagt gatattccct gcaaaattat taccggtaag catcactccc
     1621 ttgctgaatc ctgttgtacc ggaagtatag ctgatgcata ccagttccga atttggctta
     1681 tcaatatatc tgactgaatc cttgtgaaat ccgtcggagt attttttatc aaaaagctca
     1741 tttacatgtt ctttggtaag tttaccggga ttgagtggag caacaatatt ttcttttgat
     1801 tcgctctctt cgagtaaagt ggcaataggc tgattaacga gggttaccaa cctgaaatcc
     1861 gacagtgaat acacggcttt tactgttctc agattgcttt cttcgaggtt ttcccagatg
     1921 taatcgctta cgaacagcac ttttgactcc gaatgattga taatgtgatg cacatcgttc
     1981 ggattaaaat cctgtaaaat cggtattatc acagcaccgt aagtcactgt agcgaggtat
     2041 gtgatagccc aattggcgtt atttttaccg attagtgcta ttttatcatc cttctttatc
     2101 tccgtttgtt caaataagag atgtagccgg gcaatttctt cagctacctg cgcataagta
     2161 agcgttaaat tttcaccata attcgtgtat gcattcagat cccagttttt tctgaaactg
     2221 tcttcgaata agcgaatgaa gttttctttt atcattattc ttttgaattt tttaaaatat
     2281 attcgacaaa aatactaaaa ttacacacaa ttaaaaagtt tgtgcaatat tttttgactt
     2341 aattattatc tttttgaaaa gcactaaacg ctgaaaagag aaaaaataag tacctttgcg
     2401 aaatatttcg tggtacaggc accgagatga tgcatcgaga ttacaaaggt ctgaaaattt
     2461 gtcgtcggcc agtataaaca tctaatagta aaagaattaa aatgaatatt tcgtataatt
     2521 ggctcaaaga atacattaat attaatgtat caccagaaaa acttgctcag attctcacct
     2581 ccataggact tgaagtgggt ggtgttgaag aaatccagac tgtaaagggc ggcatggaag
     2641 gattggtgat tggtgaagta attacctgct ccagacacga aaactcagat cacctgagcg
     2701 ttacgacagt aaatgtggga acaggtgaac ctttacaaat agtctgcggt gctcccaatg
     2761 tggctgccgg acaaaaagtg gtggttgcta caatcggtac aaaactttat tcgggcgatg
     2821 aaagtttcac aattaaaaaa tcaaaaatca ggggcgtaga atctcacgga atgatttgcg
     2881 ctgaagatga aatcggtctc ggaaccagtc acgatggcat catggtgctt ccggcagatg
     2941 caaaacccgg tactttggcc agcgaattct ttgggataaa aaacgataca cttattgaag
     3001 tggatattac gcccaatcgc gccgatgctg tttcgcatta cggagtggcc cgcgacctgg
     3061 ctgcttattt tacgctgcat gatgaaactg taaaactcac caaaccgtcg atagatggtt
     3121 tcaaaataaa taataccgtt ttgtccattc ccgtaaaaat tgaaaacacc gaagcctgtc
     3181 cgcgttactc agcgctgaca atctcgggaa tttcagtgaa agaatcaccc gactggctca
     3241 agcaacgcct cctggcaatc gggtcaaggc ctatcaacaa cgtggtggat attaccaact
     3301 ttattttaca tgaattgggt caacccttgc atgcttttga tgctgataaa attaccagcg
     3361 gcgaagtaag ggtaaaaaat ctgccggatg gcactccttt tacaaccctc gaaggcaccg
     3421 aaagaaaact gaatccgggc gatctgatga tatgcaacgg ggatgtaccc atgtgtatag
     3481 gtggtgtatt tggcggtttt gattcgggag tgaccgaaaa cacctcgaaa gtattccttg
     3541 aaagtgccta tttcaatcct gtaagtatcc gcaaaactgc ccgccgtcat ggtttgaata
     3601 cggatgcatc tttccggttc gaacgtggtt gcgatcctga aatgactatt tatgcactga
     3661 aaagagcagc aatgctcata caggaagtgg ccggaggaat catttccagc gaaattgtgg
     3721 atgaatatcc caatcctgta caaccatttg aggttactgt taagaaaaat aaaattgata
     3781 acctgattgg caaaaaaatt ggccgggaaa acatcctgaa aatcctgaaa ggacttgaaa
     3841 tggaagtaac agccgataca gatgatgctt atcagctgaa agttccggca taccgtgtcg
     3901 atgtgcagcg tgatgtggat gtaatagagg atattctgcg tatttacgga tacaacaacg
     3961 ttgaaattgg cgataacctg gtttcgacct tgagttttgt gaaaaaaccc gacagccaca
     4021 aactacagat catcgtttca gaacaactta cagctcaggg tttcaacgaa gtgttgaata
     4081 attcgctcac caaaatcggt tattacggaa aactgacttc ataccccgaa aatgagtgtg
     4141 taaaaataat gaatccgctc agttcagatc tgagtgtatt gcgtcagact ttgcttttcg
     4201 gaggtttgga aaacatttcg cgcaacacga accaccgcaa tgccgatctg aaactgtatg
     4261 aattcggaaa ttgctaccaa tatcttgccg gcaaaaaaac agacgaccag gtgctgtcgg
     4321 cttacaacga agattatcac cttggaatat ggttaacagg caataaatat tcggatagct
     4381 ggattaatgg ttcacaaaaa tcaagcatct atgagctgaa agcttacgtt gaaaatatcc
     4441 ttgtacgtct cggttttgat ttacagaaat taaaaactgc agaatactca tccgatattt
     4501 acaccgaagc gatgagcatt aattctccaa acaagaaact cctctgcacg ttcggcatcg
     4561 taagtgatgc ggcaaaaaaa acatccgaca tagaagcaga agtgtttttt gctgatatct
     4621 actggactaa cctgttacaa acgctgaaca caaacgacgt aaaatacaaa gagttaccaa
     4681 aattccctga agtaaggcgt gatttggcat tcctgcttga tacaaatata acttttgcgg
     4741 agattgaaaa cacagcattc gaaaccgagc ggagaattct gaaaaaagta agtctgtttg
     4801 atgtgtatga aggtaaaaac cttgaaccgg ggaaaaaatc gtatgccatc agttttattt
     4861 tgcaggatga aaacaaaaca ctcacagaca gcattataga cggcacaatg aagaatatcc
     4921 aaaagaacat tgaatcgaaa ctgaatgcca gattaaggag ttaagtttac ttttaaaaac
     4981 aatttgcggg gctaaatcag cttcgcaaat tttcgtttta cacctgaaat ataatttcca
     5041 attacatttt cgttgcaatt cgcctgttaa atttcggtaa tctgatacaa taatattatt
     5101 aatttattca aatgagtgga ttttttggga gtatatcaaa aaatgattgt gtagatgatg
     5161 tgttttatgg caccgactat cattctcacc tcggtacaaa aagagccggc attgcatttt
     5221 tttcgggaaa aaatggcttt cagcgcgcta ttcacagtct ggaagatgga tatttccgga
     5281 ataaattcga aaacgacatc aaagggtttg caggtaactg cggaattgga gtcatcagcg
     5341 attccgaagc ccagcccatg gtagtaaact cccacttcgg acgtttcgga gtggcaacag
     5401 taagccggat tattaatatc aatgacctgg aagaacattt tctgaaacta caccacacat
     5461 tttcagagac gagtcagggt actgtaaatc ccactgaact catttccatg ctcatagccg
     5521 aaggaaacga ttttgtgtcg gggatagaga atgtttttga aaaagtgaag ggctcgtgct
     5581 ccatgctcat acttaccgaa aacggcataa ttgcggcacg cgataaactg ggacgaactc
     5641 cggttgtgat aggtaaaaaa aacaatgctt atgcagctgc attcgaaacc tgctctttct
     5701 ccaatctcgg ttatgagact gaaaaatacc tgggtccggg cgaaattgta aaacttaccg
     5761 ccgaaggatg ggaacaactg cgcaaaccca acgtagaaat gcaaatctgc tctttcctgt
     5821 gggtgtatta cgggtatcct ccctcatttt acgaaggaat aaacgtagaa gaaacacgct
     5881 acaggtgcgg agcagcatta gcaaaaaatg atccgataga agccgatttt gttgccggca
     5941 tacccgactc gggagtaggt catgccgtgg gctacagcca cgcaagaggc ttgccgttca
     6001 aacgtccgta caccaaatac actcccacct ggccacgaag tttcatgcct caaagtcagc
     6061 acatgcgcga gctggtggca aaaatgaaac ttctgcccaa caaatccatc attgagaata
     6121 aaaaaggaat ttttctggac gattccattg tacgcggcac tcaactgaaa gacaacgtcc
     6181 gcgacctgca cgaagccggt ataaaagaag ttaacatgcg tatagcgtgt ccgccgctta
     6241 cctatccgtg tgagtttctg aatttcagca ggtcgcgctc cagcatggaa ctggctactc
     6301 acaaagcagt aaaagaactg gagggaacag agaacgtgaa tatgaaagag tactccgatg
     6361 caggttctga aaaatatgca gcgatggtcg ataaaatccg cgaaaacctc ggccttacaa
     6421 cactgagatt tcagaaaata gaagatttgg tagaagcaat cgggctgccg aaagaaaaac
     6481 tgtgtaccca ctgctttgac ggatcaagtc atttttagtg tccaacttgc actgagaatg
     6541 ctttaaaaac ataaaagagc tgtattgtaa cagctcttct ttgctctccc tcttggactt
     6601 gaaccaagga ccctctgatt aacagtcaga tgctctaacc gactgagcta aggaagaatt
     6661 ttccacattt tttcaaatgc gaatgcaaaa gtaacggata tttttgaaat atacaatatc
     6721 tttgtaaaaa aaataacaaa aaattacatg aagaaagtat taggaatggg caacgccctg
     6781 acagacatac tgtttcggat tgagaacgat gatatactga gtaaactcaa tctggttaaa
     6841 ggcggtatgc aattaatcga aaacacacag tctgaggtga taaaatcaca ttttacaccc
     6901 ggatcggcta cgatggcaac cggaggatcg gcctccaaca ccatcaatgg aatatcgaaa
     6961 ctgggtattg aaagcggatt catagggaaa atcagcgacg acgaaatagg caggtttttc
     7021 gctctcaaca gtcttgagaa cggagtgaca ccctacatgc agaaaagtaa aacgccctca
     7081 ggacattgca caatcctggt ctcaccggat agcgaacgta ctctctgcac ttttctgggt
     7141 gcagccagcg aactgaatgc cggtgatata aaccagcatt tttttgaggg ctatcatttt
     7201 ttccatatcg aaggttatct ggtacaggac catgatttga ttcgtactgc actgaaaatg
     7261 gcgaaagatg ccggcctaat aacctccatt gatttggcca gcttcaacgt agtgaatgaa
     7321 aatctcgaat ttctgcatga aattattgaa aaatatgtgg atatcatatt tgcaaatgaa
     7381 gaagaagcga tggcgtttac aggcaaaaaa ccggcagaag cagtccgtga tatggcccaa
     7441 atgtgccgta tagcagtagt gaaaattgga aaagacggct ccctgatcca atccggtact
     7501 gatttttatg acatcgagcc gcatcaggtg aaaagtgtgg acacaaccgg tgccggtgac
     7561 ttttatgctg caggattttt gtttggactg gccaacagtt atccgctgga ggtgtgtggt
     7621 aaaatcggtt cgctcgtttc gtcgcgggtg gtggaagtag tgggcaccaa aatggcgaaa
     7681 gaaacctggg atgaaatagc tgaaaatgtc agattgctga cacagaatta gcggctgaca
     7741 cttatccggc ctgaaaaaca cattttctta cgtaccaaaa gtaatgctga ctgatggtaa
     7801 atgcgagaaa atgtgttttt ttcaatgagg tcatcctgac tttttttaaa acagtagaaa
     7861 tcaaagttaa acttgtatat tttcaggaat agtattacag cggcttcagc tctaccgtga
     7921 aatgtcgcat aataggttct tcggatacaa tttcatagcc tcgcctgatg ttgtttcttc
     7981 tgtcgaacac attcttaact gccacagcta ctacatccat gtgattgttt gtataggttc
     8041 ggcggggaat ggccagtcgg agtaattcaa gttccggaaa ccggttttgg cgtgtcaccg
     8101 gatcccggtc ggccagcaat gctcctattt ccacagtccg gattcctgct tcgagataca
     8161 attcaatggc cagcgtctgt gcaataaatt cttctgcggg cacccggctt attattttct
     8221 ttgcatccaa aaatatggca tgaccgcctg caggtctctg atagggaata ccaaattcat
     8281 ccagcctgct gcctaagtat tctacctgct taatccggct ttccaggtaa tcaaaatctg
     8341 ttccttcttc aagcccctgt gccagggctt ccatatccct gcccgacatg ccaccgtagg
     8401 tgataaatcc ctcgttgaga atgcagtata ttttacattt tttccagagt gcttctgatt
     8461 tcattgccag aaaaccaccc atattcacaa cggcatcttt cttggacgac atggtcataa
     8521 catcagcata cgaaaacatc tcgcggacga tttcccggat ggatttatcc gaataacctt
     8581 tctcgcgtgt tttaatgaaa taggcatttt cggcaaagcg ggcagaatca attaatagca
     8641 atacatcaaa tttttggcat aaccgggagg tttcacggat attttccatc gacacaggct
     8701 gtccgccggc ggtgttgttg gttacggtga gcacaacagc cggaattttc tctttcgggt
     8761 agcttttcag ggcattttca agtttctcca gatcgatatt tcctttgaac ggaatttcga
     8821 gctgcgtatc ctttgcctca tctatggtac aatccagggc agttgcttta cggtattcta
     8881 tgtgtgcctt ggtggtatca aagtgcgaat tgccaggcaa aatatcacct tcttttacga
     8941 tggctgagaa aagcaaattc tcagcagcgc gtccctgatg ggtgggaaaa aaataatcga
     9001 atcccagcaa atcgctaata gcatttttca gggaaaaata ggaccgtgca cctgcatagc
     9061 tctcgtcacc aagcatcatg gccgaccatt gcctgtcgct catggcccct gtgccgctgt
     9121 cggtgagcaa atcgataaaa acctgcccgc ttttcagtcc aaaaaggttg tatttcgcat
     9181 ctctgatcca tttttcgcgc tcatgacgag tgctccggcg gattgattcc accattttta
     9241 ttttatacga ttcggcaaac ggaagttcca ttttttcagc tgtttttttg tatttttgat
     9301 ttataatcat gcaaatttag caatttatag gagaatgcat ctttcatccg gtgaaaaaaa
     9361 gtaattttgt cggaaaaata tttttatgaa aattaatacc ggaaacgtca ggatctatat
     9421 atttattacc atcgccatgc tgtgctggtc attttcattt atctggacca aaatagcact
     9481 ggaaagtttt cctcccctca cactgatcac tcttcgcctg attctggctt cggtacttct
     9541 ctacggattt ctgaaactga gcggacgatt gcaaaaaatc gacaaaccgg atctgaaact
     9601 gttttttgca ctggctttct tcgaaccgtt tatgtattac gtgggtgaaa cttatggtct
     9661 gacaatggta aaaccaacgc tggcatcggt cattatagct acgattccgg tttttgctcc
     9721 tttttttgca tttgtatttc tgaaagagcg gtttaccatc atcaatatgc tcggaatcgg
     9781 tgtttcaatc attggtgtgt acctgatcgt ttacaatacc gacggaagtg aaaccacctc
     9841 tgtcattggc gttgcgctgg tttttctggc agtactttcg gccatcatct atggtttgtt
     9901 gcttcgtaaa atacccgaga agtattcggc caccaatatt atattctatc aatccatgat
     9961 tggtttgatc tattttattc ctgtggctgc gatggtggat ttgaggctgt tggcacgaat
    10021 ggtgatactg cgtcaatcgg tacaagcact tttgatgctt accttttttg cttcggttat
    10081 tgcgtttgtg ctgtttgcag atgtggtcag aaagatcggt attaccaaat cggaagtatt
    10141 tacaaacctg attccggtat tcactgcgct gctttcatgg gcaatcttca aagaaatgat
    10201 gaattccttt caatggattg gagtttttgt tgtaattttg ggtgtttttg tgagtcagag
    10261 taagaataaa aaagccggaa gaccgaagag tgaagacgtt agatgatatt tgaaataagt
    10321 tgtttgcttt aattaaaatg ttaaataaaa atgagtttct aagtttgaga agttaataat
    10381 ttggcaaaaa gccatggaaa ccggagaaga tctttttgtt ttgtaaaaag attttcccaa
    10441 agaagaacca tataactcaa cttctcagat ccgatgtgca gctgattcca ttgctttaaa
    10501 tattgcctaa ggttcaattt cccagtctga caaagaacaa cagaaattta tcagctattc
    10561 cattcgctca ttggcagaag ttgtaaatgg cttatataaa gcaaagaata gaaattattt
    10621 gccagaagat atattttcaa aatattataa acagtcgttt gatttaatga atatgatgat
    10681 tgcattcaaa aatcaaataa aataactttt tatcaagaca cgggcaagcc tgcaagtaga
    10741 atctttttta cagactaaaa cttcagtctt cggtcttacg tcctcaaact tgtgttatta
    10801 tgaaacttct ctacccttta tacttcgttt atcaatggct tatagctatg ccattgatgc
    10861 tgatatcaac cgttatcacc tctattctga tttttcttat ttctccggtt tttccgaacc
    10921 ataaaatttc gtatctgccg ggcatacttt ggggaaaatt tgtctgttat ctctttttca
    10981 ttcatgtaaa aataagcgga atggagcatc tggacagaaa gcaatcctac gttttcgcag
    11041 ctaaccatca gagtgctttt gatattctgg taatttacgg ctggcttcca tacattttca
    11101 agtggctgct gaagatagag ctacgtaaag ttccgtttct gggtgcagcc tgccaggcag
    11161 ccggtcatat ttttgtggac agatccaatc ctgttgctgc caagcagagc atgcaaaaag
    11221 ccgttgaaca gttgaaaaat ggcgtttcgg tagttatttt tcccgaagga acgcgaacca
    11281 aaaccggaaa actcggcaaa ttcaaacggg gtgcttttct catggcactg gagctgcaac
    11341 ttcccatcgt acccattact attgcaggta gtttcgaaag gattaaaggc tggcgaactt
    11401 ttccgggaac aataaaaatg actattcacc cccctgttga tgtgaccacc tacggaatgg
    11461 acgaatcatc agcattgctc cgaaatactc aggaaattat cagaagcgga ttgtaaatgc
    11521 ctttggcgaa atacggtctt ttttcactgc acataattca aatctcaatt gtttttttat
    11581 attttgagtg acaatatagt taaatgttgt taattctcaa tccaaacgac tgtgtgtaat
    11641 cgtctaatta tctgtaagat aaataaattg cattacaaag atgtaaatta tttatggtac
    11701 ttggtattgc atagtattat attgtattgt atatttgcag cataaattta ttaaaataag
    11761 aattttcata aaaaatccac tttaaaaatt aatcatttta aaacaagaaa actatgtcag
    11821 gcaaatacga cgatttagaa aaactgagag acctttactc caaaggcatt attaccgaag
    11881 ccgaatttaa tgccgaaaag gccaaaattc tcaatgataa ccgggaagcc ggtgtaaatg
    11941 aaactactca aacggcagat aacaacaatg gttactgctc gctcatgcac ttgtcacagt
    12001 tttcgaactt cctgtttccg ggattgggtc ttatcatccc gattgtgatg tgggctaccc
    12061 gaaaaaacga aagtaaattt gctgatgtta acggaaaaat cattttcaac tggaaaatca
    12121 gttcgtttat ctacaccagt gttctggtga tactggtaat agcagcagcg ttggtctttg
    12181 gcacacaaat agcattttca ggaattggta attatgatac tttccaggga atgatggatg
    12241 ataatccgat gaaaatattt agtttaatcg gagcattggg catcatcatc atacctgcaa
    12301 tagcaattgt tgtgcttgat ttcatattta ccatcattgg tgccatcaaa gccaacaaag
    12361 gcgaagtgtg gaattacccg ctcagcattc ggtttttcag cacaaaaaac caataataaa
    12421 acgaaaacaa atttttcaca tctaaagaag aataaaccgg atgataaaac ttaccgacat
    12481 aaacaaaacc tattataccg aagccacttc gcttcatgta ctgaaaggga tagacctgac
    12541 gatcgaaaaa ggagaatacg tttcggtgat gggtgcatcg ggttcgggaa aatctaccct
    12601 gctgaatatc attggcattc ttgacaatta tgatacagga agctactacc tgaatgatat
    12661 actgatccgg gatttaagcg aaaaaaaggc agcctggttt cgtaacgaaa tgattggatt
    12721 tgtatttcaa tcatttaacc tgatcaattt taaaaatgcg atggaaaatg tagcactgcc
    12781 gctgtattac aaaaacgtcg gacgaaagaa acgcaatgcc attgccatgg agtacctgga
    12841 tatgatggga ctgaaagact gggcaacaca catgccttct gaaatgtcgg gtggtcagaa
    12901 acagcgtgta gccattgccc gggctatcat ctcacaaccc aaagtactcc ttgccgacga
    12961 gcctactggt gcccttgaca gtaaaaccag cgaagaagta atgcaggtgt tgaaagagct
    13021 gaataaaacc ggtattacca ccgtcattgt tacacacgaa cagtcggtag cag
//
LOCUS       DAORKP010000054        12902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_451_length_12902_cov_7.636802, whole genome shotgun sequence.
ACCESSION   DAORKP010000054 DAORKP010000000
VERSION     DAORKP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_12902_cov_7.636802"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..388)
                     /locus_tag="PLN75_04515"
     CDS             complement(209..388)
                     /locus_tag="PLN75_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03414.1"
                     /translation="MLIKVCGMIHPDNIHQIAGLHPDFMGFELFPSSPRYAGNSINLS
                     ILEKIPGKTKKSVCF"
     gene            complement(701..1567)
                     /locus_tag="PLN75_04520"
     CDS             complement(701..1567)
                     /locus_tag="PLN75_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRG03415.1"
                     /translation="MKWINRNDDESNYEDRRGMSTGRKVAMGGGIGTIIMLALYLFTG
                     QDFSPLLEVFNGGSTTQTEATVDPSRAKENEDLKVFSLGVFNSANDVWTEIFRREGSE
                     YRKPVFVTFTDYTTSECGGASEQVGPFYCPGDEKVYMDLDFFHKLASQYNAPGELAIA
                     YVTAHEVGHHIQKLLGLTDKMQNYRGRVSETQYNDLSVRLELQADFYAGLWLHYAVKM
                     GTLALEPGDIESALGAANAVGDDTIQKQAQGYVVPDSFTHGTADQRMRWFMKGYRTGD
                     FNQGNTFNATTL"
     gene            complement(1676..2338)
                     /gene="ung"
                     /locus_tag="PLN75_04525"
     CDS             complement(1676..2338)
                     /gene="ung"
                     /locus_tag="PLN75_04525"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRG03416.1"
                     /translation="MDVKIEESWKKHLQAEFEKSYFGELTDFVRAEYQTKKIFPPAGL
                     IFKAFDSCPFDQVKVVILGQDPYHGIGQAHGLCFSVSDGVESPPSLVNIFREIERSYG
                     KPIPANGNLTRWAEQGVLLLNATLTVRAHQAGSHQGKGWETFTDAVIRLLSAEKNNLV
                     FFLWGNYAQQKSVFINQQKHLVLKSVHPSPLSAYRGFIGNNHFVLANEFLKKNGLNEI
                     SW"
     gene            complement(2368..3543)
                     /locus_tag="PLN75_04530"
     CDS             complement(2368..3543)
                     /locus_tag="PLN75_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HRG03417.1"
                     /translation="MKYNFDEIIDRHNTGAIKIEKCKALFGTDDLIPLWVADMDFRTP
                     DFITNSIAKRLEHPILGYTTPCEDYFKSVIHWIEKQHQWKVDRQWFGFLPGIVPGLAF
                     GVNAFTQPNDEIIVQPPVYPPFLNVPTKNDRKVVFNPLKEINGRFEMDFDDLERKITG
                     RTRLFILCNPHNPGGRIWERETLIRLAEICYKHGILVISDEIHSDMALPGNRHIPFAT
                     VSELAEQNSITYMAPSKTFNMAGLISSSYIIPNRDIRKRFSEFLENSELANGNIFAYF
                     AAKAAYENGSEWLKQMIQYIQGNLEYVVDFLQKNIPQIVPMIPQASFLVWLDCNGMNM
                     NSNELQDFMVHKAGLGLNKGTTFGPGGERRLRLNIGCPRSILEQAMKQLKKAIDELD"
     gene            complement(3630..5222)
                     /locus_tag="PLN75_04535"
     CDS             complement(3630..5222)
                     /locus_tag="PLN75_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRG03418.1"
                     /translation="MRYKFFFIFLLFFTFAFSQEKSQESPRLVVGIKIDGLQASHLHK
                     MWKYLTPGGFRKILSESMVVERMQHNIVSAGNAADAATYMTGTYPYYHGVSGDFYFSR
                     ADNEAISILYDKNQTGIGTKNKFSAHRLLTSTFTDELMLNNSLSQVHSIAIRAEDAIM
                     MGGHTATSVSWIDDITNRWITTAYYPKGLSRWADLMNVNGTFKELISEKWTPSASIST
                     YLNQTVKGSKTVPFSYNPTDRREGNSARTILANTPAANSLVTELAKTIVEKEQLGADP
                     HTDVLMLQYTVKIQNQIASSLHYAEQEDMYIRLDRNIQSLLYELSSKIGADKLYVFVV
                     GSGSDVHSPELLGKNQIPAGLFNADKSLALLNTYLMAIYGQEKWITGYYGKNIFLNKR
                     KIEDKNLNLSDFTKRITEFLVEFEGVQAAYTSAEILQFSGENSDARMKIRNSTNKNTS
                     GDIMIELMPGWIEVDNKGNVVGDANNPVVNVPFYLMGKGIKPHQVTEIYSTTDIAPTI
                     SSLMDIPFPNACTGRQIIPIEQ"
     gene            complement(5288..6418)
                     /gene="hisB"
                     /locus_tag="PLN75_04540"
     CDS             complement(5288..6418)
                     /gene="hisB"
                     /locus_tag="PLN75_04540"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRG03419.1"
                     /translation="MAEKPKRVLFIDRDGTIIAEPNNTFQVDTLDQLEFLPHVIRNLY
                     FIRHKLDFEWVMVTNQDGLGTPVYPQENFDRVQHKLLQILESEGIHFDRIFIDKSFPE
                     DNLPTRKPNTGMLTGYFNDEYDLANSYVIGDRLTDVELAANLGCKAILINENIDLTES
                     GDLASVTALQTGEWDRISEFLFAGERTATIKRKTNETDILVYLNLDGTGKADINTGLR
                     FFDHMLEQIGRHSGCDLKIEVNGDLEVDEHHTIEDTAIALGEALLQSLGNKRGIERYG
                     FTLPMDDCLCSVALDFGGRPWLVFEGEFKREYVGDFPTEMMMHFFKSLSDAARMNLNI
                     RVEGENEHHKIEGIFKAFARAVKMAIKRDIYNYQLPSTKGKL"
     gene            6847..8523
                     /locus_tag="PLN75_04545"
     CDS             6847..8523
                     /locus_tag="PLN75_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRG03420.1"
                     /translation="MDWLNSLLFGNGIAHTILIYSFVIFTGLILGKVKFFGISLGSTF
                     VLFMGILVGHFGFEVNHEILDFIKDFGLILFIYSIGLQVGPGFFSSFKKGGLTLNMLA
                     AGIVLLGVIVTTSFFFILNGRVSMPMLVGIMSGAITNTPGLGAAQEALRQAFEAGQIT
                     EIPQIALGYAVAYPLGVVGIITSIVLIRFLFKIDLQKEAHELENDDEATLEKPDKLTL
                     KFTNKAMAGKSLHHIKEMIGRKFVVSRMKRDENYFIPQSETLLQYGDILLVVASKADT
                     ESIIAIMGEELEMNWKESEKHLVSRRIVITRNEINGKTLGSLRLRSNFGVNITRVNRS
                     GIDLLGATNLVLQVGDRVMVVGELEAIEKVEKLLGNALKKLNEPPVITIFVGIFLGIL
                     FGSIPFYFPGMPMPVKLGLAGGPLIVAILIGRFGYKLKLITYTTQSANLMLREIGITL
                     FLASVGLASGSQFVETVFTSNGLMWVGIGFLITVLPLLIIGIVGRKAVKLNYYTLIGL
                     LAGSTTDPPALAYSSSIAPNDQPAVAYSTVYPLTMFLRVIMAQILILLFI"
     gene            complement(8627..9217)
                     /locus_tag="PLN75_04550"
     CDS             complement(8627..9217)
                     /locus_tag="PLN75_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRG03421.1"
                     /translation="MNLSDLLNSSVGQSIVKSVAGQFGLDEYQASAAVNTAIPVILGG
                     LNKNVQTPEGAESLNNALESKHDGSLLDNLGGILGGNVGDLLQDGGGILGHVFGNNVP
                     AVESGVAQKTGISMDKIGPIIKMLAPVVMAYLGKEKSSNNVNAGGLGDLLGGLLGGGQ
                     QQQSAGGGLFDMISGMLDKDKDGSALDDIFDMFTKK"
     gene            9432..9502
                     /locus_tag="PLN75_04555"
     tRNA            9432..9502
                     /locus_tag="PLN75_04555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9464..9466,aa:Gln,seq:ctg)
     gene            complement(10010..10083)
                     /locus_tag="PLN75_04560"
     tRNA            complement(10010..10083)
                     /locus_tag="PLN75_04560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10047..10049),aa:Thr,seq:tgt)
     gene            10334..11512
                     /locus_tag="PLN75_04565"
     CDS             10334..11512
                     /locus_tag="PLN75_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRG03422.1"
                     /translation="MDKATLHFILEHANSDVHSLMLQAAKFPEVDMPLAVRQITGMQK
                     VKNKIPTFYACKNLLYPVKLSLEQSSSEITAKHKSALCEGNVLADLTGGFGVDAYFFS
                     FRFDNIIYVEKDPELCRLAEHNFKALNRKNITVINDTAENFLKKTDSVSWIFLDPARR
                     TESGKKAVLLSDCEPNTVNLADELLSKTGHVMVKLSPMIDISVLCRELRNITEIQIVA
                     VENECREVIAILTDKASDAISIKTIHFPNSKPAETLDFDLNDEHNAVVRYADEVKTYL
                     YEPNAAVLKSGAFKLTGERFGIDKLHINSHLYTSDRMDSDFPGRIFLVEKVYDFSKNS
                     LKELQAEVKKSNITVRNFPVSVNELRKKLKISEGGDIYLFATTLKDGKKVLICCRKAK
                     "
     gene            complement(11588..>12902)
                     /locus_tag="PLN75_04570"
     CDS             complement(11588..>12902)
                     /locus_tag="PLN75_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRG03423.1"
                     /translation="MKLKLFLFFSVLISVSGWSSFAQENDKANQISVGIQVRPRAEYR
                     NGYKTPLNKDDKSAGFINQRARLSLEYQREGLSAGLSLQNVSVWGEYPISNTNKSNTV
                     VNEAWAQLKSRNGFFMKFGRQVLHYNDGRLLSVADWNQAARSHDALKLGYQTIMHQLD
                     FVLAYNQDAEKNSGGSYYYAYGVPYKTLQTLYYQYNGWKNFNPSFIFINVGLENGQPL
                     KNESKLANIQTMGTNLIYNPLKNLRLHGIGYYQSGKTKNDMNISAYLLSLKAEYDATK
                     QLKLTAGTDLLSGEEYGSPDANDSYNSFNTLYAGNHGFYGVMDLFIDAPYRTGMNVGI
                     WDKYLGTSIKISPKTALGLNYHHFSIASDAYEMMGEKLKKGLGSEIDLQFDYALLKDV
                     KLTCGYSAFLGTKTMDYVKGGDHKKWQDWAFMSINVNPRIFSTKW"
BASE COUNT         3573 a         2740 c         2610 g         3979 t
ORIGIN      
        1 ataccagcct ggaaggtata agaccataat cttctttcct gtgaatttcc tgtataaacg
       61 atttaagctt ttgttcgtcc tttacgccag gttttttctc aaatttactg ttgacatcta
      121 taccatttta ctttgtcggc actatgtatt tgaacaccgt ccaggtcata tttacagaca
      181 tttccgataa ttgtttccag attttcattc aaaaacacac cgattttttt gtttttccgg
      241 gtattttttc aagaattgac aaatttatac tgttgccggc atatcttggt gatgaaggga
      301 atagttcaaa tcccataaaa tcgggatgca ggcctgctat ctgatgaata ttgtccggat
      361 gtatcattcc gcaaactttg attaacatcg ctgtaaggtt ttaataagtt aggtttcaat
      421 acaaaattca aatttaattc tttcggaaga agtagtaagt ctggattatc tcaatttaaa
      481 aacattttta ttctttgttt tgtttcgatt atttgataat aaaacagcag ggttggtata
      541 gttgtgttac tttttaaaaa aaatcaaaaa ttgacttcag aaatattctt aatgagattt
      601 tgtttataat ttacacactt atcaaacaaa atcataccaa aaaaaagtgg atgtcctgtt
      661 ttttttgaga catccacttt ttattcgggt aataacctaa ttacaaagta gttgcgttga
      721 atgtattgcc ctgattaaag tcgcctgtgc ggtaaccttt cataaaccat cgcatacgtt
      781 gatcggctgt accgtgggta aatgaatcag gtaccacata cccctgagcc tgcttctgga
      841 tggtatcatc gcccacagca ttggcagctc cgagcgcaga ctcaatatcg ccgggttcaa
      901 gtgcaagtgt acccattttc acggcataat gcagccaaag tccggcatag aaatcggcct
      961 gaagttcaag gcgcaccgaa aggtcgttgt actgtgtttc ggaaacgcgc cctctgtaat
     1021 tttgcatttt atcagtcaga cccagcagtt tttgaatgtg atgccccacc tcgtgagccg
     1081 taacataggc aatggccagt tcacccggag cattgtactg agatgccagc ttgtgaaaaa
     1141 aatccaggtc catatatacc ttttcgtcgc ccggacaata gaacggtcca acctgttcgg
     1201 atgcacctcc gcattcggat gtggtataat cggtaaaagt gacaaaaacc ggtttgcggt
     1261 actcactgcc ttcgcggcga aaaatctctg tccatacatc gttggcgcta ttgaaaactc
     1321 ccagtgagaa cactttcaaa tcttcatttt ccttcgctct ggacggatcc acggttgctt
     1381 cggtctgggt ggtacttcca ccattgaaaa cttcgagcag tggcgaaaaa tcctgtccgg
     1441 taaacaaata cagcgcgagc ataatgatag tgccaattcc acctcccatg gctaccttgc
     1501 ggccggtaga cattccgcgg cggtcttcgt aatttgactc atcgtcgttt ctgttaatcc
     1561 atttcatggt agtatatttt ttaaaaagtt tgaaataatt tttttatcag aaaaatcaaa
     1621 ttcaggtttt ttgtttatgt tttttgtttt ttaaaaagaa taagtatttg taaaatcacc
     1681 aactgatttc atttaatccg ttttttttca aaaattcatt ggccaacaca aagtggttat
     1741 tgccaataaa acccctgtaa gccgaaagcg gcgacggatg aacggatttc aataccagat
     1801 gtttctgctg attgataaag acacttttct gctgtgcata gtttccccac aaaaaaaata
     1861 ccaggttatt cttttcggcc gagagtaacc tgataaccgc atcggtaaat gtctcccacc
     1921 cttttccctg atgcgaaccg gcctgatgtg ctcgcacagt aagcgtagca ttcagcagaa
     1981 gcactccctg ttcggcccag cgggtcagat ttccattggc aggtatcggt ttgccgtaac
     2041 ttctctctat ttcacgaaaa atattgacca gcgacggagg tgattccact ccatcactga
     2101 ccgaaaaaca caaaccgtga gcctgaccga ttccgtgata cggatcctgc cccagtatta
     2161 ccactttcac ctgatcaaac ggacaggaat caaatgcttt gaaaataaga ccggccggag
     2221 gaaatatctt tttggtctga tattccgcac gtacaaaatc tgtcagttcg ccgaaatagg
     2281 atttctcaaa ttccgcctgt aaatgtttct tccagctttc ttctatcttt acatccatcg
     2341 tttttttaat cggtctgccc tgtgagttta atctaattca tcaatggctt ttttcaactg
     2401 tttcatagcc tgctcaagaa tacttctcgg acagccgatg ttgagtctca ggcggcgctc
     2461 gccaccgggg ccaaacgtgg ttcccttatt cagtcccaat ccggctttat gcaccataaa
     2521 atcctgcagt tcatttgaat tcatgttcat tccgttgcaa tccagccaga ccagaaatga
     2581 agcctgggga atcatcggta ctatctgagg tatatttttc tgcagaaaat caacgacgta
     2641 ttccaaatta ccctgaatgt attgtatcat ctgtttgagc cactccgaac cgttttcata
     2701 ggcagcttta gcagcgaaat aggcaaagat attaccgttg gcaagttccg agttttcgag
     2761 aaattcgctg aatctcttac ggatatctct gttaggaatg atgtacgacg aactgataag
     2821 ccctgccatg ttgaaagttt tgctcggtgc catgtaggta atgctattct gctctgcaag
     2881 ttctgatacg gtggcaaagg gaatatgtct gttgccgggt aatgccatgt cggaatgtat
     2941 ttcgtctgaa atgaccagaa tgccgtgctt gtagcagatt tcggccagtc ggatgagcgt
     3001 ttccctttcc cagatacgcc ctccgggatt atgtggattg cagagaatga acaggcgtgt
     3061 tctgccggtg atttttcgtt ccagatcatc aaaatccatc tcgaaacgac cgttgatttc
     3121 tttcagcgga ttgaacacta ctttccggtc attcttggtg ggtacattca gaaacggcgg
     3181 atatacgggt ggctgcacga tgatttcatc gttgggctgc gtgaatgcat tgacgccgaa
     3241 agccagcccc ggaactatac cgggcagaaa gccgaaccat tgtctgtcca ccttccattg
     3301 atgctgtttt tcaatccagt gaatcacact tttgaaataa tcctcgcatg gcgtggtgta
     3361 acccagaatg ggatgctcca atcgttttgc aatagaattg gtgataaagt cgggagtacg
     3421 aaaatccatg tcggccaccc aaagtggtat cagatcgtcg gttccgaaaa gcgctttgca
     3481 tttttcaatc ttaatggcac cggtattgtg cctgtcgata atttcgtcaa agttatattt
     3541 catactatct ttttatctcc cctgaaaagg agaaattatg ttaaggaatt tattttaaaa
     3601 tcagaataaa tattcattaa atgtaattct tactgttcaa tgggtataat ctgcctacct
     3661 gtgcaagcat tcggaaacgg aatgtccatc agcgacgaaa ttgtgggagc aatatcagtt
     3721 gtactgtaga tttcggttac ctgatgtggc ttaatccctt ttcccataag gtagaacggg
     3781 acattaacca ccggattgtt ggcatcgccc accacgtttc ccttgttgtc cacttcaatc
     3841 cagccgggca tgagttctat cattatatcg cccgaggtat tcttgttggt tgaatttctg
     3901 attttcatcc gcgcatcaga gttttcgccg gagaactgaa gaatctctgc cgatgtataa
     3961 gcagcctgca ctccctcaaa ttctaccaga aattcggtaa tccgtttggt gaaatcactc
     4021 agattcagat ttttatcttc aattttcctt ttattcagga aaatattctt tccatagtat
     4081 cctgtgatcc atttttcctg accataaatg gccatgaggt aagtattgag aagcgcaagt
     4141 gatttgtctg cattgaagag tccggccgga atctgatttt taccgagcag ttcaggtgaa
     4201 tgtacatcgc tcccacttcc gaccacaaaa acatataatt tgtctgctcc tattttagag
     4261 gataattcat acaacagcga ctggatattt cggtcgaggc ggatatacat atcttcctgc
     4321 tcggcatagt ggagcgaaga agctatttga ttctgaattt tcacagtgta ctgaagcatc
     4381 agcacatcgg tatgcgggtc ggcgccgagt tgctctttct ccacgattgt tttggcaagt
     4441 tcggtaacaa gcgaattagc tgcaggagta ttggcaagta ttgttcgggc agagttgcct
     4501 tcgcggcgat cggtcggatt gtagctgaaa ggaactgttt tcgaaccttt tacggtttga
     4561 ttcagatagg ttgatatgga agcagaagga gtccatttct cactgatcag ttctttgaat
     4621 gttccgttca cgttcatcag gtcagcccat ctggagagtc ctttcgggta gtaagcggta
     4681 gtaatccacc tgttggtgat gtcgtcaatc cacgagaccg atgtagccgt gtgtcctccc
     4741 atcatgatgg catcttcggc ccggatagca atgctgtgca cctgcgatag ggaattatta
     4801 agcatgagtt catcggtaaa tgtagatgta agtaaccggt gagccgaaaa tttgtttttg
     4861 gtaccgatgc cggtctgatt tttatcatac aggatagaaa tagcctcgtt gtcggctctg
     4921 ctgaagtaaa aatcgccgct cactccgtga tagtaaggat aggtaccggt catataagta
     4981 gctgcgtcgg cagcattacc ggctgataca atgttgtgct gcatgcgttc cactaccatg
     5041 ctttccgaaa gtattttacg gaaacctccg ggagtgaggt atttccacat tttatgcaaa
     5101 tgacttgcct gcaaaccatc aattttgatt cctacaacca accggggaga ttcctgactt
     5161 ttttcctgcg aaaaagcaaa ggtgaagaat aacagaaaaa tgaagaaaaa tttatatcgc
     5221 attgttttga ttgtaagttt gatactatta tcagtggttt cagggcgatg acattgattc
     5281 ttttttgtta caattttccc tttgtacttg gcaactgata attgtagatg tcgcgtttta
     5341 ttgccatttt tactgctctg gcaaaagctt tgaaaatacc ctctatctta tgatgttcgt
     5401 tctcgccttc caccctgata ttcaggttca tacgggccgc atcgctcagc gatttgaaaa
     5461 aatgcatcat catttcagta ggaaaatcgc ctacatattc gcgcttgaat tctccctcaa
     5521 acaccagcca cggcctgcct ccaaaatcga gcgctaccga acacaggcag tcatccatag
     5581 gcaaggtaaa gccataccgt tcaatgcctc tcttattgcc cagtgactga agtaaagctt
     5641 ctccgagtgc tattgcggtg tcttcaatgg tatgatgttc atctacttcc aggtctccgt
     5701 tcacttcaat tttcaggtcg cagccggagt gacggccaat ctgctcaagc atgtggtcga
     5761 agaatctcaa acctgtattt atatccgctt ttcctgttcc atccagatta agataaacca
     5821 gaatatctgt ttcatttgtt tttcttttta tagtagcagt gcgttcgcct gcaaaaagaa
     5881 actccgaaat tcgatcccat tcaccggttt gcaacgcagt gaccgatgct aaatctccac
     5941 tttcggtcag atcgatattt tcattgatta atatggcttt acatcccaga tttgcagcta
     6001 attccacatc agttagccga tcgccgataa cgtatgaatt tgccagatca tactcatcgt
     6061 taaagtatcc ggtaagcatt ccggtattcg gttttcgggt gggaagattg tcttccggaa
     6121 aagatttatc gataaatatc ctgtcgaaat gaatgccctc gctctccagg atctgcaata
     6181 atttgtgctg taccctgtca aaattctcct gcggatacac cggagttccc aatccatcct
     6241 gattggtcac catcacccat tcaaaatcga gtttatgccg gatgaaatac agattccgga
     6301 tcacgtgagg taaaaattcg agctgatcaa gcgtatcaac ctgaaaagta ttgtttggct
     6361 ctgctattat cgttccatcg cgatcaatga aaagtactct ttttggtttt tcagccatat
     6421 ataaatgtat tttgcgtcat tttattcaag atttttgcaa tctaagatgc aaagatacaa
     6481 attacttcag aaaaattaaa tattcaaaat aactatcact tcaataaatt tttccggtat
     6541 gaattgaagt agttttgact ttttttcttt tttgtttttt aactacgaat aatacgaatg
     6601 acccgaaaac attcgttatt gtaagtcttt tgattcgttt tacccgcctg cgtcggacag
     6661 gtttgtgtaa tttgtagttt gtatgttaaa tatttttaag ttattaataa accgggacga
     6721 gctcgtaatt aaaacagcac gataagtaag ttgattaaat aaaataaaat tcttaccttt
     6781 gcaccctcat ttgagaaaca aaaataaata aattaaattt cttaaaatca gatatttaaa
     6841 ttagctatgg attggttaaa ttcattatta ttcgggaatg ggattgccca cactatcctt
     6901 atttactcct ttgtaatctt tacgggatta atactgggta aggttaaatt cttcggaatc
     6961 tctctgggtt ctactttcgt attgtttatg gggattttag tcggacattt cggcttcgag
     7021 gtgaatcatg aaatactcga ttttataaaa gatttcggat tgatactgtt catttattcc
     7081 attggtttgc aggtaggtcc gggttttttc tcttcattta aaaagggagg tctgacgctc
     7141 aatatgctgg ctgcagggat tgtattgctt ggcgtgatag tgaccacttc atttttcttc
     7201 atacttaacg gacgtgtcag catgcccatg cttgtaggta tcatgtcggg cgcaataacc
     7261 aatactcccg gactcggagc tgctcaggaa gcccttcgtc aggcttttga agcgggacaa
     7321 atcacagaaa ttcctcagat agctttaggt tacgccgtgg catacccact tggggttgtt
     7381 ggcattatca cttccattgt tctgattcgt tttcttttca aaatcgacct tcaaaaagaa
     7441 gcgcatgaac ttgaaaatga cgacgaagct accctcgaaa aacccgacaa actgacctta
     7501 aaatttacca acaaggcgat ggcaggtaaa agtctgcatc atatcaagga gatgattggt
     7561 cggaagtttg ttgtatcgag gatgaaacgc gacgaaaatt atttcattcc gcaatcggaa
     7621 actttactcc agtacggtga tatattgtta gtagtagcat caaaagctga cactgaatcc
     7681 atcattgcca tcatgggtga agagttggaa atgaactgga aagaatctga aaaacacctg
     7741 gtttcgagac gcattgtgat cacccgaaac gagataaacg ggaaaacact cggttcgctg
     7801 agattaagat caaatttcgg tgtcaatatt acacgggtga atcgttcggg tatcgacttg
     7861 ctgggagcta ccaatctggt gttgcaggta ggcgaccgcg tgatggtggt aggcgaactg
     7921 gaagccatcg aaaaagtgga aaaactgttg ggtaatgcac tgaaaaaact gaacgagcca
     7981 cccgtaatta ccattttcgt aggtatattc ctcggtattc tgttcggtag catacctttt
     8041 tatttccccg gaatgcctat gccggtaaaa ctgggtctgg caggcggtcc gcttattgtt
     8101 gccattttga tcggtcgttt cggatacaaa ctgaaactca ttacatatac cacacaaagt
     8161 gccaatctga tgcttcgtga gattggtatt acacttttcc ttgccagcgt cggactggct
     8221 tcgggcagtc agtttgtcga aaccgtattc acgagcaacg gacttatgtg ggtaggcatc
     8281 ggatttctga ttaccgtttt accattgctt atcatcggaa ttgttggcag aaaagcggtg
     8341 aaactgaatt attacacact cataggttta ctggcaggca gcaccaccga tcctcctgct
     8401 ctggcatatt cgagctccat tgctcccaat gatcagccgg ctgtagccta ctctaccgta
     8461 tatcctctca ccatgtttct gagggtaata atggcacaga ttctgattct gctgtttatc
     8521 taaatcagat tttttggtaa aactttattt tatttcaatt ggcttataaa ataacaaatg
     8581 ccggatttcg aaagaaaatc cggcatttaa tattctggtt atcagtttat tttttcgtaa
     8641 acatatcgaa aatatcgtcc aaagcacttc cgtctttgtc tttatcgagc atgcctgaaa
     8701 tcatatcaaa aagacctcct ccggcacttt gttgctgttg tccgccgcct aacaagccgc
     8761 ccaacagatc gcccaaacca ccggcattca cattattgct gcttttttct ttaccaagat
     8821 atgccataac taccggagcc agcattttga taataggacc gattttatcc atactgatgc
     8881 cggttttctg agccactccg ctttctacag ccggtacatt atttccaaat acatgtccca
     8941 gaatgccgcc gccatcctgc agaagatcac ctacatttcc tccgagaatg cctccaagat
     9001 tatccagcaa acttccgtcg tgttttgatt ccagcgcgtt attcagactt tcagcacctt
     9061 caggtgtctg tacattcttg ttcaagccgc ccaaaataac cggaatagcg gtattcactg
     9121 ctgctgaagc ctgatattcg tccaaaccaa actgaccggc aacactctta acgatagact
     9181 gacctaccga actgtttaat aaatcagata aattcatgtt attcttgttt tataatttaa
     9241 gaaaatattt tttcaaatat acgtgttata tttgtaaata gtacccaaaa tctcactttt
     9301 ttttgtgaag attactaatt gactgaaaat gaaatgtttt tcgaaaaaaa tcagattata
     9361 acattttttt tatactaaaa ttttttacca gtttactaaa attttatatc tttgcacccg
     9421 taaatcaaca atgtcctata gcgtaatggt agcgcagcag attctggatc tgttagtcta
     9481 ggttcgagcc ctggtaggac aacactaaac cgcaatacaa tatactgtgt tgcggttttt
     9541 cgtttatttt ggggacaaat tgttttacct tcgtctttcc ggttatgatc tttgatggta
     9601 aaaaagtgtt gaactgacgt ccggttgttt gatttatcaa ttgacaatta atactcaaat
     9661 ttccatttca aatcattgaa aataattctt ccagattatt gaattaatta tttctactta
     9721 cgacaacttt tactcttttg aattactccc tttctgacac attttattga acagacccac
     9781 agatttttgg acagtacccg aaaaatcagg caccattact tcgaaaaaat tacagtaagc
     9841 aataaaccgg gtaataacaa acagaaaaga tgtaacacaa tgctgataag taatataata
     9901 ccgaaaagtt gtattacaat ggaaaataga agtgatttag aggtaaaaag aaccgcttac
     9961 aatttaaaac gaaaaagagc attcagggga atactctttt tttgttttga gcctcttgtc
    10021 ggattcgaac caacgacccc gagattacaa atcacgtgct ctggccagct gagctaaaga
    10081 ggcaagtggg taagccccct gcatcgcacc gctacaacct aatacccttg ctgcggtcaa
    10141 gccctggggg agttcaaagg gagctggtcg tgcaggactt acccgggcac aaaagtacta
    10201 catttcgtcg atatgacaaa atattcatct gtatttattt attaaaatgt ccttgcagcg
    10261 catcattgag tgtttttgaa atttttccgg ttctgaataa tattcattac ctttgattgc
    10321 taaacgaatc ccgatggaca aagcaacact tcatttcata ctcgagcatg caaatagcga
    10381 tgttcattca ctaatgcttc aggctgctaa atttcccgaa gtggatatgc cgttggctgt
    10441 gaggcaaatc acgggtatgc agaaggtaaa aaacaagatt cccacctttt acgcctgtaa
    10501 aaacttactg tatcctgtga agttatcgct tgaacaatcg tcgtccgaaa taaccgcgaa
    10561 acacaaatca gcactctgcg aagggaacgt actggcggat cttacagggg gcttcggagt
    10621 agatgcgtat tttttttcct tccggtttga taatataatt tatgttgaga aagatccgga
    10681 actttgtagg cttgctgaac ataatttcaa agccctgaac agaaaaaaca tcacggtgat
    10741 aaacgatacg gctgagaatt ttctgaagaa aacagacagt gtttcctgga ttttcctgga
    10801 tccggcgcgc aggaccgaat ccggaaaaaa agcggtgctc ctctccgact gcgaacccaa
    10861 cacggttaat ctggccgatg aactgctatc caaaaccggg catgtgatgg ttaaactctc
    10921 gccgatgatt gatatttctg ttttgtgcag ggaattgagg aatatcactg aaatacaaat
    10981 agttgcagta gaaaacgaat gcagggaagt catagcaata cttactgata aagcatcgga
    11041 tgccatcagc atcaaaacaa ttcattttcc aaattcaaag cctgctgaga cactggattt
    11101 tgatttgaat gatgaacata atgcggttgt acggtatgct gatgaagtta aaacgtatct
    11161 ttacgagccc aacgctgcag tattgaaaag cggtgcattc aaactcaccg gtgagcgctt
    11221 cggaatagat aaattgcata ttaacagcca tttatacaca tcggatcgta tggattccga
    11281 ttttccgggc aggattttcc tcgttgagaa ggtttatgat ttcagtaaaa actctctgaa
    11341 agaattgcag gcagaagtta aaaagtcgaa tatcacggtg cgaaattttc cggtgtcggt
    11401 aaatgaatta cgtaaaaaac tgaaaatcag tgaaggtgga gatatttatt tatttgccac
    11461 taccctgaaa gatggaaaaa aggtattgat ctgttgccga aaagcaaaat aaaaagagca
    11521 gaaattccgc aatgaaatgc tgctcttctt atttcagata acttgagagg ctgttttcag
    11581 ttacttctta ccattttgtc gaaaatattc ggggattcac gttaatagac ataaatgccc
    11641 agtcctgcca ctttttatga tctccccctt ttacataatc catcgtttta gtgcccagaa
    11701 aggctgaata tccgcaggtg agttttacat ctttgagtaa agcatagtca aattgcaggt
    11761 cgatttccga gcccaggcct tttttgagtt tctctcccat catttcgtat gcatcactcg
    11821 cgatggagaa atgatgataa ttaagtccta aagctgtttt tggcgatatt ttgattgatg
    11881 ttccgaggta cttgtcccag attcccacat tcatgccggt tctgtagggc gcatcaataa
    11941 ataaatccat cacgccgtaa aatccgtgat ttccggcata aagggtattg aatgaattgt
    12001 aagagtcatt tgcatcgggc gaaccatatt cctcgccact cagcaggtcg gttccggcag
    12061 ttaatttaag ctgtttggtt gcatcgtatt cagccttcaa cgacaacaaa taggcggaaa
    12121 tattcatatc gtttttagtt tttccgctct ggtagtagcc tattccgtgt aaacgaagat
    12181 ttttcaacgg attgtaaatc agattcgtac ccatggtttg gatattagca agttttgatt
    12241 cgttcttcaa cggttgtccg ttttctaatc ctacgttgat aaaaataaac gaaggattga
    12301 agtttttcca tccattgtat tgatagtaaa gcgtttgcaa tgttttgtaa ggcactccgt
    12361 aagcataata ataacttcct ccgctgtttt tctccgcatc ctgattatat gcaagcacaa
    12421 aatcgagctg gtgcataatg gtctgataac ccagtttcag cgcatcatgt gagcgggctg
    12481 cctgattcca gtcggcaacc gacagcagtc gtccatcgtt gtaatgcagc acctgacggc
    12541 cgaatttcat aaagaaaccg ttccggctct tcagttgcgc ccatgcttcg tttactaccg
    12601 tattgctttt gttcgtgttc gaaatgggat actcgcccca cacacttaca ttttgcagac
    12661 taagtcctgc cgagagtcct tcgcgctgat attcaagcga taaacgtgca cgttgattga
    12721 taaatccggc tgatttatca tctttgttca gcggtgtttt gtagccattg cggtattcgg
    12781 cccgtgggcg cacctggatg ccaacactga tttgatttgc tttatcattt tcctgagcaa
    12841 acgaactcca accggatact gaaatcaaga cagagaagaa taagaataat tttaacttca
    12901 ta
//
LOCUS       DAORKP010000055        12667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_469_length_12667_cov_9.947669, whole genome shotgun sequence.
ACCESSION   DAORKP010000055 DAORKP010000000
VERSION     DAORKP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_12667_cov_9.947669"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1135)
                     /locus_tag="PLN75_04575"
     CDS             complement(194..1135)
                     /locus_tag="PLN75_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRG03424.1"
                     /translation="MTYIPFKPNQTGTFYIQDIPLKEIAQFIDWRFYFLAWRLTGQFA
                     GIEKVHDCPSCEAGWLQGFDEKDRPKAREALKLFRDTQEMLRDVLENDLLKVHAVYAI
                     YPAHAEGDNLVIETENETVILPVLRQQKPSADGFCYSLADFLSDENDYLGAFANTVTG
                     TEEMAQKFEQENDVYKSILVKTLSDRLAEATAEWLHRKVRKEFWGYAPDENLSTEEML
                     KTQYKGIRPAVGYPSLPDQSVIFELDKLLHYHKTGISLTENGAMYPNASVSGLYFAHP
                     LSRYFMIGKIDENQLADYALRKGKNPEEMRKWLAANI"
     gene            complement(1375..2439)
                     /gene="corA"
                     /locus_tag="PLN75_04580"
     CDS             complement(1375..2439)
                     /gene="corA"
                     /locus_tag="PLN75_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRG03425.1"
                     /translation="MSRNTRKRKEDIGLSPYEMKFRGQKKADKTTMDLFDFNLEFVHE
                     LQVKSTEKLKSYKHNDTISWLNVNGLDDVTLMKELTGIFGIDNNIMSDIMNPAIRPKV
                     QEFEQGIMVSLKMLQFNERTNKLSVENLCLVISHDFLISFQEVKGDFFDTVRERIRKH
                     KNKIRTAGTDYLAFALLDMVVDNYIYIIGLLGEKIENLEERMTGNVNKQLPELINLYK
                     QELNFLRRHIKPAKEMILTLAKMESEFIHAENRVNYKELQDNINEANDLTDGYREMLY
                     DLLNIYHSAATTRLNDIMKLLTIISVIFIPLTFIVGVYGTNFDFFPEIRWHYGYFAMW
                     GVMMVVAGFMIWYFKRKDWF"
     gene            complement(2488..3078)
                     /locus_tag="PLN75_04585"
     CDS             complement(2488..3078)
                     /locus_tag="PLN75_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03426.1"
                     /translation="MQFPLNFTFKISSIANDFRVHDAAGVEVAYVRQKVFKLKEEVQV
                     FNNESRSQVNFTVKATKWIDFSPTYLFFDAQGMQTGSVVRQGMKSIWRASYVICDEKG
                     KADLSINEENAVVKVFDGIFGELPLIGILAGYFFNPTYLVTRTNGLPVVRLTKEKSFF
                     GRRFKVDKLAEFSSTGEENRIMLGLMMMILLERQRG"
     gene            complement(3091..3549)
                     /locus_tag="PLN75_04590"
     CDS             complement(3091..3549)
                     /locus_tag="PLN75_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03427.1"
                     /translation="MNTSKSFINRLKPGIPKRVLLFVAGLVWTFAGGMLLYRGGRLLA
                     DYPEYLWIKLFLSIGAGILFYYFLFDKISAKHTARIKSLPHERPCMFSFFDPKSYLMM
                     GLMISMGIGLRMSGLVALKHISLLYVTMGIPLTISAIRFYLNGLFFRKNQ"
     gene            3764..4216
                     /gene="aroQ"
                     /locus_tag="PLN75_04595"
     CDS             3764..4216
                     /gene="aroQ"
                     /locus_tag="PLN75_04595"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRG03428.1"
                     /translation="MKKIIIINGPNLNLLGKREPDVYGNQDFENYFNRLKASYPDVEL
                     DYFQSNVEGEIINKLHETGFSFDGIILNAGAYTHTSIAIADAIRGITVPVVEVHISNV
                     FSRENFRHHSYLSEVCRGVIVGFGLDSYRLAIESYQAASLFGGKLVKL"
     gene            4606..4947
                     /locus_tag="PLN75_04600"
     CDS             4606..4947
                     /locus_tag="PLN75_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRG03429.1"
                     /translation="MARKIIFFEEHFIEFYKTLDNKVKEKVKYVFELIKQVERVPEKF
                     LAPIKGYEGLFEIRIEFQSNIYRIFCCFDQGQLVILFNGFQKKTQKTPKEEIERAMRL
                     KTVYFQTNKEK"
     gene            4947..5246
                     /locus_tag="PLN75_04605"
     CDS             4947..5246
                     /locus_tag="PLN75_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRG03430.1"
                     /translation="MMEYKNITNFDDLIELEHGKLGTESRNEYEERAQMFIVSEMLKE
                     ARREANLTQEELAVRAGTKKSYISKVENAKGNIQLSTLIRIFEKGLNRKIGLTFL"
     gene            5550..6998
                     /gene="pyk"
                     /locus_tag="PLN75_04610"
     CDS             5550..6998
                     /gene="pyk"
                     /locus_tag="PLN75_04610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRG03431.1"
                     /translation="MEQKKFTKIVATISDKRCDVDFISQLYEEGMNVVRMNSAHLNRE
                     GFLKIINNVRAVSNRIALLIDTKGPEVRTTVAENDAIELKTGEFIRIAGNPEMMSTKD
                     CIALSYPNFVRDLHVGDDILIDDGELDLKVVSKDDEFLTCMVLNDGMLGSRKSVNVPG
                     VRINLPSLTEKDRQNILFAIEQKLDFIAHSFVRSKQDLIDIQEILDAHKSQIKIISKI
                     ENQEGVDNIDEIIQHCYGVMVARGDLGIEVPQERIPGIQRKLIRKCVKAHKPVIVATQ
                     MLHSMIENPRPTRAEVNDIANAIYYRTDALMLSGETAYGKYPVEAVRTMSKIALEAES
                     TKMPENDIPIEYGRGDVSAFMAHAAVEAGANIDIKAIITDTFSGRTARFLASYRGSDP
                     ILAFCYNERASRELALSYGVYSYYKEELVEDERTYFITLLQNLIEREIISSEDLICYL
                     SGPISGSFGTTFLEINTAKVLLSEIDNYTMPK"
     gene            7222..9666
                     /locus_tag="PLN75_04615"
     CDS             7222..9666
                     /locus_tag="PLN75_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRG03432.1"
                     /translation="MKKLILFSLFIVFTVATKAAVNIQGRIINADTQQPIEFVTVSIY
                     NPSTSKNIAGALTNQDGVFNIVSVANGTYELRISFVGYSPFNKEFKVNGTDVKFGNIP
                     LTEDSKMLKEVQVVGQGSQMRFDIDKKVFSVDQNIAAAGGSATDVLQNIPSVDVDNDG
                     NISLRNNASVEVWINGKPSGLTADNRAQVLQQMPAENIESIEVMTNPSAKFNPEGTAG
                     IINLVMKKNRKAGYYGSLSAGATVQNGKPGYNAGASINYNVGKFDVFANLGYRRMAGN
                     GWGLTERTNYNNGDSTLLNQNSKTEFKMGGVFFRAGVDYHIDAKNTIGINGFGHNGKP
                     QMNSETDYLLINKASTNKLRDYSRTNDGSDEHKGYNVNLYHKIDFDKKGSNLMTNLSY
                     SEHGNLGNQRFIQDDRLLPDNDQDITQIADGNSNTIELKTDYTKKFTDNNRLELGWSS
                     IWNDRLSNASGTDNFKNRDIPEYYNVFDYAEQIHAGYATYGTRIDNFSVQAGVRGEYF
                     RKDITSTSSRESIKLDPQSYFKVYPSVYLSYSLPNNNELQLNYTRRVDRPRGRMVNSF
                     RDYSDSTNISYGNPLLMPEMTSAFELNYIKNWDNHTLSSSVYYRFNNDDIERVSFIRN
                     GVLESTFMNLTKEHDLGVELVAKNRLFKILNLTSSLNFYYNKLDASVYTNELYPEVRV
                     VLPAHENFSWDGRVIANLMLSPTFSGQITGQYRSEQLTPQGKQLESYSMDLGLRKSFF
                     EKKFSVNFNVRDLFDSRKFRSVTSGEGFYQYSENKRMGRMFGLTLTYNFGNMKPKPVE
                     RPKSSDSGESDEMNME"
     gene            9817..12339
                     /gene="feoB"
                     /locus_tag="PLN75_04620"
     CDS             9817..12339
                     /gene="feoB"
                     /locus_tag="PLN75_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRG03433.1"
                     /translation="MKLSELQTGQKASIVKVLGHGGFRKRIIEMGFVKGKIVESLQNA
                     PLNDPVKYRVMGYEISLRRSEANLIEVVSEEEALKTLKPEKKADVFFHDEEVRKAALI
                     KRKEITVALVGNPNCGKTTLFNFASGSREHVGNYSGVTVGAKDATFVQDGYRFRIVDL
                     PGTYSISAYSPEEKFVREFLLNETPDVIINVVDSSNLERNLYLTTQLIDMHLQTVVAL
                     NMYDELQTSGDKLDYKALGKLLGMPFVPVIAKNGFGIVQLFRSVIKMYESTHIINEEG
                     ILIQSVKDDKLLDEYHHEVQIEHQHDKKQSEADLIVKQDIRNFQISRHIHINYGPELE
                     KSIVRLRNLLVIENHHLQAKYSFRYLSIKLLENDPVVNDQIAQETNAHKVLKIRDEEV
                     RRIQSTLKTDVESAVTDFKYGFIAGALKETYTPASKKNKNTLTSKLDNVLIGKYWGYP
                     LFIFFIFIMFQATFTLGQYPMDWIESGVEWLSRVLRNSMNEGPLKALIIDGILGGVGG
                     VIVFLPNILILYFFISLMEDSGYMARAAFIMDKIMHKMGLHGKSFIPLIMGFGCNVPA
                     IMATRTIENRNSRIITILVNPLMSCSARLPVYLLLAGTFFPGQAGLVLFGIYATGIIL
                     AVGVARLLKKYVLKRDETPFVMELPPYRIPTAKSMWKHTWEKGQQYLRKMGTVILVGS
                     VVIWFLGYYPRPDAGTEMTEKQQTTQLEKSYIGQVGHAIQPVLAPLGFDWKISVSLLS
                     GVAAKEIVVSTLGVLYSGNAEAETQSLASRMKTDVRNDGTAVYTTGTALAFMLFVLIY
                     FPCLATIVAIKNETASWKWAFFAVAYSLGLAWLVAYLAQLIF"
BASE COUNT         3824 a         2611 c         2718 g         3514 t
ORIGIN      
        1 ccgaatatat gagaataagt ataaattatt ctatgttttc tattgtttta aaaaaaagtc
       61 aagatgactt cattgattaa aagatataag acgtcagaca aaaaatcaaa tcacaagaat
      121 aagaagctgt ttttttttct ttaagtcccc cttcggggtc ccgatagcta tcgtggatag
      181 ggggcctctg ctttcatata ttcgctgcca gccacttgcg catttcctcc ggatttttgc
      241 cctttcggag ggcgtagtca gccagctgat tttcgtcaat tttcccaatc atgaaatacc
      301 tggagagcgg atgggcaaaa tacaacccgc tgaccgatgc attgggatac atggctccgt
      361 tttctgtaag cgaaatgccc gttttatggt aatggagcag tttgtccagt tcaaaaataa
      421 ccgactgatc cggcaacgaa ggatatccta ccgccggtcg gatgcctttg tactgcgttt
      481 tcaacatttc ttctgtcgat aaattttcat cgggagcata tccccaaaat tctttccgca
      541 ctttccggtg gagccactcg gcggtggctt ctgccagtcg atcggagagt gtttttacaa
      601 gaatggattt gtacacatca ttttcctgct caaatttctg agccatttct tcggtgccgg
      661 tcacggtgtt ggcaaatgca cccaggtaat cattttcatc gctcagaaaa tctgccagcg
      721 aataacagaa tccgtcggcc gatggttttt gctggcgaag tacaggcaat attaccgttt
      781 cgttttcagt ttcgatcacc agattatcgc cctcagcatg agccggataa atggcatata
      841 ccgcatgtac tttcaacagg tcattttcaa gcacatcccg aagcatttcc tgagtatctc
      901 tgaagagttt caacgcttcg cgggctttcg gacggtcttt ttcatcgaaa ccctgaagcc
      961 aaccggcctc acacgacgga caatcgtgta ctttttctat acccgcaaat tgcccggtca
     1021 gtcgccaggc aaggaaataa aaacgccagt cgataaattg agcgatttct ttaagaggaa
     1081 tgtcctgaat ataaaaagtc cctgtttgat taggtttgaa aggtatgtaa gtcataatgt
     1141 gtgatacccg ccttcggatg acgaaataaa aatgtagtgt cgaattagtt tgaaataagt
     1201 atttatttaa aaaaaagaag aatatcatta ttcaaacaaa aaaacaccgt atttgttcca
     1261 cctgcagctc aaatctgtga aaaaaactat tcgtccccat tcgcgcaatt cgttataatt
     1321 cgcgcaattc gtactaattc gtgtaattcg tattaattcg tgtaattcgt attatcagaa
     1381 ccagtctttg cgcttaaagt accaaatcat aaaaccggcc actaccatca tcactcccca
     1441 catggcaaaa tagccgtaat gccaccttat ttcagggaaa aaatcgaaat tggtaccata
     1501 gactcctacg atgaatgtaa gtggaatgaa aatgacggaa atgattgtga gcagtttcat
     1561 aatatcgttc agccgggtgg tagctgccga gtgatatata ttcagcaggt cgtagagcat
     1621 ttcgcggtag ccgtcggtca gatcgttggc ttcgttgatg ttgtcctgca gctctttgta
     1681 gttaacgcgg ttttcagcat ggatgaattc cgattccatt tttgcaagtg tcagaatcat
     1741 ctctttggcc ggtttgatgt gtcggcgaag aaaattcagt tcctgcttgt acaggttgat
     1801 aagctcgggc agttgtttgt tcacatttcc ggtcatgcgt tcttccaggt tttcaatttt
     1861 ctcgcccagt aatccgatga tgtaaatata attatccacc accatatcca gcaatgcaaa
     1921 tgccaggtag tcggttcctg ccgtgcgtat tttgtttttg tgcttccgga tccgttcgcg
     1981 aaccgtatcg aaaaaatcgc cttttacttc ctgaaatgaa atcaaaaaat cgtgacttat
     2041 caccaggcag aggttttcca ccgatagttt gttggtgcgt tcgttgaact ggagcatttt
     2101 gaggctcacc attattccct gttcgaattc ctgtactttg ggtcggatgg ccggattcat
     2161 gatatccgac atgatattgt tgtctatccc gaaaattccg gtcagttctt tcatcagcgt
     2221 tacatcgtcc agtccgttta cattgagcca gctgatggtg tcgttatgct tgtaggattt
     2281 cagtttttca gttgatttta cctgaagttc gtgtacgaat tccaggttga agtcaaacaa
     2341 atccatcgtt gtcttatcgg ctttcttttg ccccctgaat ttcatttcgt agggcgagag
     2401 gcctatatct tcctttcgtt ttctggtatt tcttgacatg gtattttatt ttttttttga
     2461 aaaaaaacgt atgatgtcgt gtggtactca tccgcgttgt ctttcgagca gtatcatcat
     2521 catcaggccg agcataatcc ggttttcctc gccggtgctg ctgaattctg ccagtttgtc
     2581 gaccttgaag cgtcggccga aaaacgattt ttcttttgtc aggcgcacta ccggtaagcc
     2641 attggtgcgg gtaaccaggt aagtgggatt gaaaaaatag ccggctaaaa tcccgatcag
     2701 gggcagctct ccgaaaatcc cgtcaaaaac ttttaccacg gcgttttctt cgttaattga
     2761 aagatcggct ttcccttttt catcacaaat aacgtatgat gccctccaga tggacttcat
     2821 gccctgcctt accacgctgc cggtttgcat tccctgagca tcgaaaaaaa ggtaagtggg
     2881 tgaaaaatca atccattttg ttgctttcac agtgaaattg acctgcgagc gactctcgtt
     2941 gttgaatacc tgtacttctt ctttcagttt gaatactttc tggcgaacat aagctacttc
     3001 aactcctgca gcatcatgaa cccggaaatc gttggctatg gaagaaatct taaaggtgaa
     3061 attcagcgga aattgcatgt taaagtagtt ttattgattt ttacgaaaaa aaagtccgtt
     3121 cagataaaat cttatcgccg agatagtcag cggaattccc atcgtaacgt acagcagcga
     3181 aatgtgtttg agagccacca gtccgctcat acgcaaacct atacccatcg aaatcatcag
     3241 ccccatcatc aggtagcttt tcggatcgaa aaacgaaaac atacacggac gttcgtgagg
     3301 aagcgatttg atgcgggcgg tatgtttggc tgaaatttta tcaaacagga agtagtaaaa
     3361 caaaatgccg gcaccgatgc tcaaaaacaa ttttatccat agatattcag gataatcggc
     3421 cagcagcctg ccgcccctgt aaagcaacat tccacctgca aaagtccata ccagtccggc
     3481 aacgaaaagc agaacacgct tcggaattcc gggcttgagt cgattgatga atgattttga
     3541 agtgttcatg aatatttttt caaaaatacg gaattaaatc agtttcaaaa aaatctttcg
     3601 atataaaaat tatgaaagtg aaagaaattt gataaaaatg aagaactaaa tacccgtgct
     3661 gtcggttcgt ttggcgtaac tccgaaaacc tgtagccggt aaatttttta aaccgatgaa
     3721 aatgttgtaa ttttgtggtc gaattcaata acaaaacact ccgatgaaga aaataatcat
     3781 tatcaacgga ccgaatctga atctgctcgg caaaagagag cccgatgtgt atggaaatca
     3841 ggattttgag aactacttca accggttgaa agcgtcttat ccggatgtgg aacttgacta
     3901 ttttcagtcg aatgtggagg gagaaatcat caacaaactc cacgaaaccg gatttagctt
     3961 cgatggtatc atcctcaatg ccggagctta tactcacacc tccattgcca tagccgatgc
     4021 catcagagga atcacagttc cggtggtgga ggtgcatatc tccaacgtgt tttcccgtga
     4081 aaacttccgg catcattctt acctttcgga ggtgtgccgc ggggtgatag ttggtttcgg
     4141 gctcgattcc tacaggctgg cgatagagag ttatcaggct gcatcgttat tcggtggtaa
     4201 gttggttaaa ctctaaataa ttgttagttt ggttccggcg ttaataaaac ggaaaatatc
     4261 caggacaata aagccattag atttcactgt ttttctgttt gtcaatggat ttatcacaac
     4321 agatttgatt ttaaagttga aaaacggacc tattttcagc ttgatacgat tggtaaacaa
     4381 tgggtgcgtg ctgttaattc tgtaagctaa aataccggaa aaattatttg aaagaaacac
     4441 gtaaattgac cagggaaatt ttttattatt tttcacgcgg ttccttgtat gaaagtaaaa
     4501 atttgcctcc aaaaagcatt taaagcgtat aattaaaaga aaaaagttca tttgtgagat
     4561 aacaaaagga aattattgtt atctttgcag ataacaaaaa aaacaatggc aagaaaaata
     4621 atattttttg aggaacactt cattgaattt tataagacat tggataacaa agtcaaagaa
     4681 aaggtgaaat atgtttttga attgataaaa caggttgaac gagttcctga aaagtttttg
     4741 gcacccatta aaggttatga aggtttattt gagattcgaa ttgagtttca atccaatatt
     4801 taccggattt tctgttgttt tgaccaggga caattggtga tacttttcaa tggatttcag
     4861 aagaaaaccc aaaaaactcc caaagaagaa attgaacggg caatgagatt aaagacagtg
     4921 tattttcaaa ctaataaaga aaaataatga tggaatacaa aaatataaca aattttgacg
     4981 atctgattga acttgaacat ggtaagttgg ggacagaaag tcgaaacgaa tatgaggagc
     5041 gtgcccaaat gttcattgta agtgaaatgt taaaggaggc aagacgtgag gcaaacttaa
     5101 cacaagaaga attggcagta agagccggga caaagaagag ttatatctct aaagttgaga
     5161 atgccaaagg aaatattcaa ctgtcaactt tgattcggat atttgaaaaa ggtttgaatc
     5221 gaaagatagg attgaccttt ttataaataa ttacacccca caacatacac taagggcaac
     5281 ccggtcgggt attcgtttag taatattcgc tattaacaaa aagcagaatg actatgtaac
     5341 gcatattcaa ttactcaaaa atcacaatgc cgtcattatc aaattaaagc aacttcacta
     5401 aatcaattga aaaattgatg caaaacgaaa caaaaataac gcgaaaccaa taattcgcta
     5461 tacgcaaata acagcgaagc taactaatgg tgttgccagg ctacgtgaat tcaataacgt
     5521 gcgtagcaca aataacacga agtattcaaa tggaacaaaa gaaatttaca aaaatagtag
     5581 ccactatcag cgacaaacgc tgcgatgtgg attttatcag ccagctttac gaagagggaa
     5641 tgaatgtggt gcggatgaat tctgctcacc tcaaccgcga aggttttctg aaaataatca
     5701 acaacgtaag agccgtaagc aacagaattg cgttgctcat tgacaccaaa ggacccgaag
     5761 tacgtaccac agtagcagaa aacgatgcca ttgaactcaa aaccggtgaa tttatccgga
     5821 ttgccggtaa cccggaaatg atgtcaacaa aagactgtat agcattaagt tatcctaatt
     5881 ttgtgcgcga tttgcatgtt ggcgatgata tcctcattga cgatggtgag ctggatctca
     5941 aggtcgtatc gaaagacgat gaatttctta cctgcatggt actcaacgat ggaatgctcg
     6001 gcagccgaaa aagcgtgaat gttcccggtg tacgtatcaa cctgccatcg ctgactgaaa
     6061 aagaccgcca gaatattctc tttgccattg agcaaaaact ggatttcata gctcattctt
     6121 ttgtgcgctc caaacaggat ctgattgata ttcaggaaat tctcgacgct cataaaagtc
     6181 agataaaaat tatttcgaaa atcgaaaatc aggagggtgt tgataatatt gatgaaatca
     6241 tacagcactg ttacggcgtg atggtagccc gcggtgacct ggggatcgag gtgccgcagg
     6301 aacgtattcc cggcatacag cgcaagttga tacgcaagtg tgtgaaagca cacaagccgg
     6361 ttatcgtagc cactcagatg cttcattcca tgattgaaaa cccgcgtccg acccgtgctg
     6421 aagtcaacga tattgccaac gccatttatt accgcaccga tgcactcatg ctgagcggcg
     6481 aaacagctta cgggaaatat ccggtcgaag ccgtacgcac gatgtcaaaa attgctttgg
     6541 aagccgaaag tactaaaatg cccgaaaacg acattccgat tgaatacgga aggggagatg
     6601 tgagcgcatt catggctcac gcagcagtag aagccggtgc caatatcgat atcaaagcca
     6661 tcattaccga tactttcagt ggcagaaccg cacgattcct ggcttcatac cgcggttcgg
     6721 atccgatact tgccttttgc tacaatgaac gtgcttcgcg cgagctggca ttgtcatacg
     6781 gagtgtattc ttactataag gaagaacttg tagaagatga gcgcacctat ttcatcactt
     6841 tattacagaa tctgattgaa cgggaaatta tctcttccga agatctgatc tgctacctca
     6901 gcgggccaat cagtggcagc tttggtacca cattcctcga aataaacaca gctaaggtgc
     6961 tgctttccga aattgataat tatacaatgc caaaataatt tcgttataaa ttaagggatt
     7021 acaacctgaa acgataattg cggttatccc gaatccttaa aactctgcct acgtctcagc
     7081 cgcctgcaga gtttttgttg ttaatatacc tttttatttt ttttgaaatt aaactttttg
     7141 cgtagaaaat tgtcttagta tagatatttt aatttttgct gtttttttgg ataaaaatca
     7201 gcacaattaa caaaaacatg tatgaaaaaa ctaattttat tttcactttt tatcgttttc
     7261 actgttgcaa caaaagctgc tgtcaatatt cagggacgca tcattaatgc cgacacgcag
     7321 caacccatcg aatttgtaac agtctcaatt tataatccgt ctacttccaa aaatatagca
     7381 ggcgcactca ccaaccagga cggagtgttc aacatagtat ccgtggccaa cggaacgtat
     7441 gaactccgta tcagttttgt gggttacagc ccttttaaca aggaatttaa ggttaatggc
     7501 accgatgtga aatttggaaa tattccactt accgaagact caaaaatgct aaaagaagtg
     7561 caggttgtgg gtcagggctc gcagatgcgg ttcgatattg ataagaaagt ttttagtgtc
     7621 gaccagaata tagcggctgc cggaggatct gccaccgatg tgctccagaa tattccttcg
     7681 gtggatgtag ataacgatgg caatatctcc ctgcgcaaca atgcgagcgt ggaagtgtgg
     7741 ataaacggaa aaccctccgg tcttactgcc gacaaccgcg cccaggtgct acagcaaatg
     7801 ccggccgaaa acattgaaag catagaagtg atgacaaatc cttcggcaaa atttaatccc
     7861 gaaggtaccg cgggcatcat taacctggtg atgaaaaaga accgaaaagc aggttattac
     7921 ggcagccttt cggccggagc gaccgttcag aacggtaaac ccggctacaa tgccggtgca
     7981 agtatcaact acaatgtggg caaattcgat gtttttgcca atctgggata ccgcagaatg
     8041 gcaggaaacg gctggggact caccgaacgc acaaactaca ataacggcga ttctaccctg
     8101 ttgaatcaga atagtaaaac agaattcaaa atgggaggtg tgttcttccg tgcaggcgtg
     8161 gattaccaca tcgatgctaa aaatacaatc ggtatcaatg gattcgggca caacggcaaa
     8221 ccgcaaatga attctgaaac cgattatttg ctcatcaata aagcttcaac caacaaactt
     8281 agagactatt cacgcaccaa tgatggctcc gacgaacaca aaggctataa cgtaaatctt
     8341 tatcataaaa tagattttga taaaaaagga tccaatctga tgaccaatct ctcgtactcc
     8401 gagcacggca atctgggcaa ccagcggttc attcaggatg atcgactttt accggacaat
     8461 gatcaggaca tcacgcaaat agccgatggt aacagcaata caatagagct taaaacagat
     8521 tataccaaaa aatttactga caataacagg ctcgaactcg gctggagcag tatctggaat
     8581 gaccggttaa gcaatgcaag cggaactgac aatttcaaaa acagggatat tcccgaatat
     8641 tacaatgtat ttgattatgc cgaacaaatt catgcagggt atgccactta cggaacacga
     8701 atagataatt tttccgttca ggcaggtgtg aggggtgagt atttcagaaa agatattacc
     8761 tccacttcga gtcgtgagtc aataaaactt gacccgcaat cctattttaa ggtgtatccg
     8821 agtgtgtacc tttcctattc attgcccaat aacaatgaac ttcagctgaa ctatacccgc
     8881 cgtgttgacc gtccccgtgg acgcatggtc aattctttcc gcgattactc ggattctacc
     8941 aatattagtt acggtaaccc attgctgatg cccgaaatga cctcggcttt cgaactgaat
     9001 tacataaaaa actgggataa tcataccctc tcatcttcag tgtattatcg gtttaataac
     9061 gatgatatcg aacgggtaag cttcatccga aacggagtgt tggaatcaac cttcatgaac
     9121 ctcaccaaag aacacgacct gggagttgaa ctggtggcta aaaaccggct tttcaaaatt
     9181 ctgaatctta cttcttcact caatttttac tacaacaagc tggacgcatc tgtatatacc
     9241 aatgaattat atcccgaagt cagggttgtg ctgccggcgc atgaaaattt ctcctgggat
     9301 ggccgggtga tagctaatct gatgttatcg cccactttct ccggtcagat taccggacag
     9361 taccggtcgg aacaactcac accacagggc aaacaactgg agagctattc catggattta
     9421 ggtttacgta aatcgttttt cgaaaagaaa ttcagtgtca atttcaatgt aagagactta
     9481 tttgattccc gaaaattccg aagtgtgacc agcggagaag gattctacca atattccgaa
     9541 aataaacgca tgggacgcat gttcggactt acacttactt ataacttcgg aaatatgaag
     9601 cccaagcccg ttgaacgtcc caagtcctca gattcgggag aatcagacga aatgaatatg
     9661 gagtaagctg caataataaa ggaataaaac aggaaataac gttttgtatg ttttacagaa
     9721 cgttattttt ttttgtacag gaaaagaaat agccggtttg ctcttgttta gagacaatct
     9781 aaatagtatc tttgtgcttc atttaagaaa aacagcatga aactttccga acttcaaacc
     9841 ggtcaaaaag catctattgt gaaagtgctt ggtcatggcg gtttcagaaa acgcatcatt
     9901 gaaatgggtt ttgtgaaagg aaaaattgtg gaatcactgc agaatgctcc tctcaacgac
     9961 cccgtgaagt atcgtgtgat gggatatgaa atatcattgc gccggagtga agccaatctg
    10021 atcgaagtgg taagcgagga ggaagccctt aaaacgctta aaccggaaaa gaaagcagac
    10081 gttttttttc acgatgagga ggtgcgtaaa gctgctttga taaaacgaaa ggaaataacg
    10141 gttgctcttg tcggaaatcc gaattgcgga aaaaccacgc tgtttaattt tgcgtccggt
    10201 tcacgcgaac atgtgggaaa ttacagcgga gtgactgtgg gtgccaaaga cgctactttc
    10261 gtgcaggatg gttaccggtt tcgcattgtt gatttgcccg gaacttactc tatatctgca
    10321 tattcacccg aggagaaatt tgtgcgcgaa tttctcctta acgaaactcc cgatgtgatc
    10381 atcaacgtgg tcgattcgtc gaacctggaa cgaaacctgt accttaccac ccagcttatc
    10441 gacatgcacc tgcagacggt agtggcgcta aacatgtacg acgaactgca gacaagcggc
    10501 gacaagctcg attataaagc acttggcaag ttgctcggaa tgccttttgt acccgtaata
    10561 gctaaaaacg gtttcggcat cgtacaacta tttcgcagtg tgattaaaat gtatgaatcc
    10621 acgcacatca tcaacgaaga agggattctg atacaatctg taaaagacga taaactgctg
    10681 gatgaatacc atcacgaggt tcagatagag caccagcacg ataagaaaca atcagaagcc
    10741 gatcttattg tgaaacagga tatacggaat tttcagattt cacgtcacat tcacatcaat
    10801 tacggacctg aactcgaaaa gagtattgtg agacttcgaa atttgctggt catcgaaaat
    10861 catcaccttc aagcgaaata ctcgtttagg tatctttcca tcaaattact tgaaaatgac
    10921 ccggttgtca atgatcagat tgctcaggaa accaatgctc acaaagtact gaaaattcgt
    10981 gatgaagagg taaggcgcat tcagtcaacc ctcaaaacgg acgtggaatc ggctgtaacg
    11041 gattttaaat acggttttat agccggagca ctcaaggaaa catacactcc ggcctcaaaa
    11101 aaaaataaaa acaccctcac atcgaagctc gataacgtac tcataggtaa atactggggg
    11161 tatccgctgt ttattttctt tatttttatt atgtttcagg ccactttcac gctgggtcag
    11221 tatccgatgg actggataga gagcggagtg gaatggctga gccgtgtgct cagaaacagt
    11281 atgaatgaag ggcctctgaa agcgctcatt atcgacggaa tacttggcgg tgtgggaggt
    11341 gtgattgtgt ttttacccaa cattctgata ctgtattttt tcatttcact catggaagat
    11401 tcgggttata tggcgcgggc tgcatttatt atggataaaa ttatgcataa aatgggactg
    11461 cacggcaaat ctttcatccc gctcattatg ggtttcggtt gtaatgtccc cgctattatg
    11521 gctacccgca ccattgagaa ccgaaacagc cggatcatta ccatcctggt taatccgctc
    11581 atgtcatgca gcgccagatt accggtatat ctgttgcttg ccggaacatt tttccccggt
    11641 caggccggac ttgtgctttt cggaatttat gccaccggaa tcattctggc ggtgggtgta
    11701 gccaggctac tgaagaaata cgtattgaag agagacgaaa ctccgtttgt gatggaactg
    11761 ccgccgtacc gtatcccgac tgcaaaatcc atgtggaaac acacatggga aaaaggacag
    11821 cagtatcttc ggaaaatggg taccgttatt ctggtcggtt ctgttgtaat ctggttttta
    11881 ggatactatc cgcgtcctga tgcaggcact gaaatgactg aaaagcaaca aaccacgcag
    11941 ctcgaaaaat cctatatagg tcaggtgggt catgccattc agccggtgct tgcaccgctc
    12001 ggtttcgact ggaaaatcag tgtttcgctg ctttcgggcg tagcagccaa agaaattgtg
    12061 gtaagtacgc tgggtgtgct ttattcaggc aatgccgagg ctgagactca gagccttgca
    12121 tcaagaatga aaaccgatgt ccggaatgac ggaactgccg tttatactac cggaacagcg
    12181 cttgccttca tgttgtttgt actgatttat tttccctgtc tggccaccat tgtagccata
    12241 aaaaatgaaa ccgcaagctg gaaatgggct ttttttgctg tagcctactc gctggggctc
    12301 gcctggcttg ttgcttattt ggcacaattg attttctgat agatactgtt ttttaaaaac
    12361 aagcaaaaac ggttttagat gaactttttg aaaaataact tgttatacat gcaaaactaa
    12421 ttgatgcaac aactaatagt catagtgatt ggagtgctgg tttttggtta tgtagtttat
    12481 caaacctaca aaacccttac aaacaaaagc acctcctctg atgtaaaatg cggtggatgc
    12541 aatagttgtt cgccaataag tgaaaaataa gtgtgtgtga aaattaaaaa acgggacgat
    12601 gtgaattgtc ccgtttttgc ttttttatat cctgcaataa tgttgctttt tgtttacctg
    12661 gcgcagt
//
LOCUS       DAORKP010000056        12636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_474_length_12636_cov_7.990700, whole genome shotgun sequence.
ACCESSION   DAORKP010000056 DAORKP010000000
VERSION     DAORKP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_12636_cov_7.990700"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..935)
                     /gene="panC"
                     /locus_tag="PLN75_04625"
     CDS             complement(87..935)
                     /gene="panC"
                     /locus_tag="PLN75_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRG03434.1"
                     /translation="MIIETVKELREYLAGQRAAGKTVGLVPTMGFLHEGHLSLIREAK
                     AENDVVVVSDFVNPTQFGPNEDFESYPRNIERDTELALSAGADVIFYPSVSEMYPPES
                     STYVEVEGNITRVLCGASRPTHFRGVTTVVNKLFNIVEPNKAYFGQKDAQQVAVLTKM
                     VKDLHMDIELIICPIVREPDGLAMSSRNTYLSAEERKQAVILNQALKKAKNAFANGEK
                     DVVKLTGIITDKINEMPLAVIDYVSILDFPSLKPIITIEKDALAAVAVKFGKTRLIDN
                     EILGYS"
     gene            complement(938..1771)
                     /gene="panB"
                     /locus_tag="PLN75_04630"
     CDS             complement(938..1771)
                     /gene="panB"
                     /locus_tag="PLN75_04630"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRG03435.1"
                     /translation="MEKKISVKSFQKAKDENRKITILTAYDYSMAKILDEAGIDSILV
                     GDSLGMVFQGNESTLPVTVEDIIYHTRAVTRGVSRALVIADMPFLSYHISKDEAIRNA
                     GRIIKEGGAEAVKIEGGAYFAHTISAIVQSQIPVMGHIGLTPQSVNVFGGFKVQGRST
                     ESAKQILEDAKILEDAGVFAITLEGVPEELAKLIAESVTIPVIGIGAGKYCDGQVLVV
                     NDMLGMYSDFVPKFVKQFAKLNVEIKAAVQEYISEVQSGSFPAEENTYGIDQEIIDKL
                     Y"
     gene            complement(1778..2596)
                     /locus_tag="PLN75_04635"
     CDS             complement(1778..2596)
                     /locus_tag="PLN75_04635"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRG03436.1"
                     /translation="MIAFVGAGKVATALGNYFRTKGIDVAGYYSRNFGHARQAAEITN
                     TRPFANLQELINSSRMIWITTSDDAIEEVAIQIAALNIPPSENKMFLHTSGVHSTGIL
                     LPIKEKGFSTGSAHPLLSFSGEPTTVEKLNHTWFTIENDELTEFFKKTNNPVIKIDGK
                     QKALYHTACSILANYMVTLMDISFSIFEKTGIEKDKIRNAAIPLLESVIQNTQYKAGK
                     DALTGPLKRGDENTIRMHLQSLEKDMPGLVEIYKTLGRETMKILDDYRLSEILK"
     gene            complement(2721..4505)
                     /locus_tag="PLN75_04640"
     CDS             complement(2721..4505)
                     /locus_tag="PLN75_04640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRG03437.1"
                     /translation="MDSYVVSALKYRPSTFRSVVGQDALTTTLKNAIKSKKLAHAYLF
                     CGPRGVGKTTCARIFAKTINCEHLTEEFEACNQCESCKSFNDQRSYNIHELDAASNNG
                     VDDIRTLTDQVRIPPQIGRYSVYIIDEVHMLSQGAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISVTDTVNHLKYVASQENVNIEESALNIVAQKSDGGLRD
                     ALSIFDQLVSFSGNHITYRDVIENLNVLDYEYYFRLTNSFLGGNVSESLLIFNEILNN
                     GFDAHHFITGLSSHLRDVLVCKDPVTIQLLEVGAEIAERYRRQAADCEAEFIFSALKI
                     SNDCDLNYRISKNKRLLVELTLIKICQLAGDKKKILTGKESAPLQKTTPAPAQNDEKK
                     ESPALTAETKTEYPAAKTTTSENIVAETVVAETAKIPTDKKPAPSVNSEPKFQRPVSI
                     SISSLKNEKAETPHPIPELPIEDEGNVGLFTDKDLTSRWKDFIALHNEDPGTKALLEN
                     HYPELTKENTVKVMVDNKFQEKELMQMGVVNYLRKELKNDSIKLEIELAPESEFKKHQ
                     TPQEIYKRMANDFPLLEKLRKNLDMEID"
     gene            complement(4612..5637)
                     /locus_tag="PLN75_04645"
     CDS             complement(4612..5637)
                     /locus_tag="PLN75_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRG03438.1"
                     /translation="MKIRHIYMVFLMVALLTAGCEKTIKFRGEIMNPMLVVSSFVTPD
                     SAIKAVLTESRFFLDEGSGGYGGYEYNKVTNADFSLYVNNTFKEKLTHTGAGIYRAAY
                     RPKPGEEIRIEAVASGFESVKATTVIPPQPVILSVDTSSVTEKQYYIGYTGEYNGSNR
                     QDTLSEYTYKNIRFRVKLRDDGNKKNYYRLVVRKRTYYENEVNESYLFDFEDIVFGNQ
                     QTNDIGSLFEFSDNNYFFDTFTDDLFNGKEYDLKFSNSIRISQKDYKGYDSQFRKEMR
                     STYYIYLQEISHDYYMYARSSAAAGNQSGNPFTEPVQIYTNIANGIGIFGSYASSAPA
                     VIDYRNE"
     gene            complement(5642..8098)
                     /locus_tag="PLN75_04650"
     CDS             complement(5642..8098)
                     /locus_tag="PLN75_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03439.1"
                     /translation="MQIRKIIILISLVVWSITLHAQRYTISGYITDKSNGETLISASV
                     FDEISKKGTVTNNYGHYSISLNAGEVDLNYSYVGYTTEKSNFRLTKDTVINVKMDASI
                     ALREVTVVGRRNEMGVQGTQMSAIEVPIEQIKNIPTLFGENDLIKALQLLPGVQSGTE
                     GSAGLYVRGGGPDQNLMLLDGIPIYNVNHMFGFFSAFNTDAVKNVTLYKGSFPARFGG
                     RLSSVVDVRMKDGDDKNFHGNVSVGLISSKFNLEGPIIKEKTTFNISARRTYIDFLSQ
                     PIIKRAAKENSGDETGNTTAGYYFYDLNAKLTHKFSEKDKLYLSAYLGDDAIYARYRT
                     SSTSYGDRNTSDWMKLNWDWGNAIAALRWNHVVTNKLFMNTTAAYTRYRSNMNVGYLS
                     ESTDKSINPPKTRTEEFRVGYRSGIEDVSLKADFDYAPNTAHDIKFGVNYTYHTFRPD
                     VSTFYFFNNYNDKNETMDTVIGKGNIYAHETMTYLEDNITLTDRFKLNAGIHFSTFNV
                     QQKNYFSIEPRLGMRMLINEQLSVKAGYAHMNQYIHLLSNNSISMPTDLWVPATRFIE
                     PMKSDQYAFGIFYNLKNLLDFSLEGYYKSMNNLIEYKDGASFMGESSGWEEKVVMGRG
                     WSYGVEFLAQRSFGNTTGWLGYTWSKTERLFDRPGQELNYGKVFPAKYDRRHDLSITV
                     MHKFSEKIDISGNWVFATGNTATLGMQTYQGTVIPETNQGYTFRGPETYISSRNNYRY
                     NNYHRMDLGVNFHKKKKRGIRTWNISIYNVYNQYNPFMVYQSSEGSYNPRTGLYESSQ
                     VLKQVTLFPIIPSVSYSYKF"
     gene            complement(8563..9660)
                     /locus_tag="PLN75_04655"
     CDS             complement(8563..9660)
                     /locus_tag="PLN75_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03440.1"
                     /translation="MKTKKIVAALVVLTFAIITTTPANAVTTPPWAPAGGYNAKTTHV
                     FLPDQNMYFDLKKNVYIYEENGQWFNSPTLPQKFSEVDFNNAKQIEIEMKGENPYTKN
                     LEHREAFNKQIKAEQDAYLKEQKQKLDEAERLQKEAEKAQKESEKAQKVVDKAQQESE
                     KAQEKAQTAQKEIDKVNEKAAQEAEKARVNAEKAQQKIDAANAKAQEKTDKAAQKAAK
                     EAEKAQKLIDKANANTAKAEAKAQKEKEKAAAKAAKEAEKSQKSIDKAKAQAAKAEAK
                     AQKEKAAAEKKAAKEAAAQQKQIDKANKEAAKAEAKAQKEAKKLQKEVEKAQKNADKA
                     QKNAEKAQKKANIIQNATPPAAPQEAPAPNQ"
     gene            complement(9857..10336)
                     /locus_tag="PLN75_04660"
     CDS             complement(9857..10336)
                     /locus_tag="PLN75_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03441.1"
                     /translation="MKTKELIVAFAAMFALTIAIPAESAAAPPYWAPANGYRAKNTHI
                     YFPQQNFYYDLHRGVYIYMNGRNWSTSAALPVMYRNINLSVVPQVQLVINNHRPYAYN
                     SNHRAKYWNRKAQHDYYKRMEKHQRTYSKAMYKADKKYHRANKKMVKKYYKQNKNHR"
     gene            complement(10466..11410)
                     /locus_tag="PLN75_04665"
     CDS             complement(10466..11410)
                     /locus_tag="PLN75_04665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRG03442.1"
                     /translation="MSEDSTLFKVFSGTKSRYLAEKICKSLNWPLGKMNIEHFSDGEF
                     SVSYEESIRGQYIYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSIGAKLIADMLTVAGVDRVITMDLHADQIQGFFNVPVDHLYASTIFIPYIKS
                     LALENLVIASPDVGGSKRAGSYSKHLEVPMVLCHKTREKANVVGEMRIIGDVEGKNVI
                     IVDDMVDTAGTLCKAANLMIDNGALSVRAIVSHGVMSGIASDNITNSKLTEIAFTDSI
                     PFDCTKCAKVKILSIADMFADTIRRVQEHKSISEQYLL"
     gene            11693..>12636
                     /locus_tag="PLN75_04670"
     CDS             11693..>12636
                     /locus_tag="PLN75_04670"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRG03443.1"
                     /translation="MKIHVNIPYFTRWGQRILVSGNVKELGDGDFSKALPLSLNSHEN
                     WYGEFECNPPATEPLKYKYLLFDENTGKYTEEWGDNRTVELQKNTDDYYCFDSWNVAG
                     SVENVFMTSPFRKVLLKPGIAALAKAKQPAKYTHKFIVKAPLLKPNQALCIIGNCKQL
                     RNWTVSDPAMMNLRDDEWELKIDLSKATTEIHYKYGVYDAEAGHFLFFEQGPDRIAAV
                     IQNSVVQTNDGFIRVDNQSWHGAGLGLPVFSIRTRKSFGVGDFADLKLLVDWSEKIGM
                     KLIQLLPLNDTTGTHTELDVLPYAAISAFALNPLFLNLP"
BASE COUNT         2969 a         2676 c         2822 g         4169 t
ORIGIN      
        1 ataatgtaag taacataaga ttatttacga tttacgattt acgattttac gatttacgat
       61 tgaaatcttt ccctttaatc aactattcaa ctataaccca gaatttcatt atcaatcaac
      121 cgcgttttgc cgaattttac agccacagca gccagagcat ctttttctat cgtaatgatt
      181 ggtttcagcg acggaaaatc gaggatgctc acatagtcaa taacggctaa cggcatttcg
      241 ttgattttat cggtaatgat tccggttagt tttaccacat ctttttcgcc gttggcaaat
      301 gcatttttcg cttttttcaa cgcctgattt agaattaccg cctgctttct ttcttcggca
      361 gaaagatagg tatttcgcga actcatggca agcccgtcgg gttcgcgtac tatgggacaa
      421 ataatgagtt caatgtccat gtgcagatct ttcaccattt tcgttaatac agctacctgc
      481 tgggcgtctt tttgtccaaa atacgctttg ttgggctcaa cgatattaaa cagcttattc
      541 acaacagtag ttacgcccct gaaatgagta ggtcttgatg ctccgcaaag cactctcgtg
      601 atatttcctt ccacttccac gtaagttgaa ctttcggggg gatacatctc gctcaccgaa
      661 ggatagaata tcacgtcagc acctgcgctc agagcaagtt cggtatctcg ttctatgttt
      721 cttggatacg actcaaaatc ctcgttgggc ccgaattgcg taggatttac aaaatcgctt
      781 accaccacca catcattctc cgcttttgct tcacggatta acgacagatg gccttcatgc
      841 agaaaaccca tggtgggtac caaacccacc gttttaccgg ctgcacgctg accggcaaga
      901 tattctctta attcttttac agtttctatt atcatttcta atacagttta tctataattt
      961 cctgatcgat accgtatgtg ttttcttcgg ccggaaaact tcccgactga acttcgctga
     1021 tatactcctg aacggcggct ttaatttcaa cattcagctt tgcgaattgt ttaacgaatt
     1081 taggcacaaa atcactgtac atacccagca tatcattgac tacgagcacc tgcccatcac
     1141 aatatttacc tgcgcctatt ccaatgaccg gaatagtaac cgattcggct attagtttgg
     1201 caagctcctc aggcacccct tcaagcgtga tggcaaatac acctgcatct tccagtattt
     1261 ttgcgtcttc cagtatttgt tttgcgcttt cagtgcttct tccctgtacc ttaaatccgc
     1321 caaatacatt caccgactgg ggtgtgagcc cgatgtgtcc catcacggga atttgtgact
     1381 gaacaatggc agaaatggta tgggcaaaat aagcgcctcc ttcaattttt acggcttcag
     1441 ctccgccttc tttgataatt ctgcctgcat ttcggatggc ttcatctttg gaaatgtggt
     1501 aagagagaaa tggcatatcg gcaatgacca gtgcacggct tacacctctc gtcacagccc
     1561 gtgtatgata aatgatgtct tccacagtga ccggaagcgt cgattcgttt ccctgaaaca
     1621 ccattcccag cgagtcgccc accaggatgg agtcaattcc ggcttcgtcg agaattttcg
     1681 ccatggagta atcataggct gtgagtatgg ttattttcct gttttcatcc ttggcttttt
     1741 gaaatgactt gacactgatt ttcttttcca tgatttgtta ttttaatatt tcgctgagtc
     1801 tgtagtcgtc caggattttc attgtttccc ttcccagcgt tttgtaaatt tccaccaatc
     1861 cgggcatatc tttttccaaa ctttgcagat gcatccggat ggtgttttcg tcaccccgtt
     1921 tcagaggtcc tgtaagagca tcttttccag ctttgtattg tgtattttga attacacttt
     1981 ccagcagtgg tatcgcagca tttcgtattt tatctttttc aatacccgtt ttctcaaaaa
     2041 tgctgaagga aatatccatc aacgttacca tgtaattggc aagtatactg catgccgtgt
     2101 ggtataaagc cttctgtttg ccatcaattt ttattaccgg attgttggtt tttttgaaaa
     2161 attcagtcaa ctcatcattc tcaattgtaa accaggtgtg attcagtttt tctaccgtag
     2221 ttggctcgcc gctgaaggat agtaaagggt gtgcgctgcc ggttgaaaat cctttttctt
     2281 tgattggcag aagtatgccg gtagagtgta ctccgctcgt atgcaaaaac attttatttt
     2341 cactgggagg tatgttcagg gctgcaatct gtatggcaac ttcttcaatg gcatcgtcgc
     2401 tcgtagtgat ccagatcatg cggcttgagt ttatgagttc ttggaggtta gcgaacggcc
     2461 gggtattggt aatttcggca gcttgtcttg catgcccgaa gttgcggctg taataacccg
     2521 ctacatctat acctttggtt ctgaaataat tacctaaggc agtcgccact ttcccggcac
     2581 ctacaaaagc aatcatgctg gatgatgttt taagtaagaa atacttttta ctttcagggt
     2641 gcaaagttac gaataattat agcaaaggac aattattcgc tgacgtttgt gtgtaaagta
     2701 gtacccggag cgggttgaaa tcagtcaatt tccatgtcca ggttcttgcg aagcttttcg
     2761 aggagcggga aatcgtttgc cattcttttg tagatttcct gaggagtctg atgttttttg
     2821 aattcagatt cgggcgccag ttcaatttcc agttttatgg agtcgttttt caattccttt
     2881 cgcagataat tcaccacacc catctgcatc agttcctttt cctgaaactt attgtcaacc
     2941 attactttaa cggtattttc ttttgtcaat tcaggatagt gattttccag cagggctttt
     3001 gtgcccggat cttcgttgtg cagggcaatg aaatctttcc acctggaagt caggtcttta
     3061 tcggtaaata atcctacatt accctcatct tcgatcggga gttcggggat aggatgtggc
     3121 gtttccgctt tctcgttttt cagtgaggaa atggaaatac ttaccggacg ctgaaatttc
     3181 ggttcggagt tcacagaagg tgccggtttt ttgtcggttg gtatttttgc tgtttcggcg
     3241 acgactgttt cggctacaat gttttcagac gttgttgttt tagctgccgg atactctgtt
     3301 tttgtttcag ctgtaagtgc agggctttcc ttcttttcgt cgttttgagc aggagcgggt
     3361 gtggtttttt gtaggggggc agactcttta ccggtcagta tttttttttt atcaccggcg
     3421 agctgacaaa tttttatcaa tgtcagttct accaacagcc ttttgttttt actgattcga
     3481 taattcaggt cgcagtcgtt gcttattttc agcgccgaaa aaataaattc ggcttcacag
     3541 tcagcagcct gccgcctgta tcgttcggca atttcggcgc ccacttccag cagctgtatt
     3601 gtcaccggat ctttgcatac aagtacatcg cgcaggtgcg aactcaatcc ggtaatgaaa
     3661 tgatgtgcat caaacccatt attcagtatt tcgttgaaaa tgagcagcga ctcgctaaca
     3721 ttaccgccaa gaaaagagtt ggtaagcctg aaatagtact cataatcgag tacgttgaga
     3781 ttttcgatta catcccgata ggtaatgtgg tttcccgaga aacttacaag ctgatcaaag
     3841 atagaaagtg catcgcgcag gccgccgtca cttttctggg ctacgatgtt cagagcgctc
     3901 tcctcgatgt ttacattctc ctgtgaggca acgtatttca gatgatttac cgtgtcggta
     3961 accgaaatac ggttgaaatc ataaatctgg caccgcgaaa gaatcgtagg gataatttta
     4021 tgcttttcgg tggtagccaa tatgaagatg gcatgtgcag ggggctcttc cagtgttttc
     4081 agaaacgcat tgaaagcgcc ctgcgaaagc atgtgtacct cgtcgatgat gtatacgctg
     4141 tatcggccta tctgcggtgg tatccgaacc tggtcggtga gggtgcgtat atcgtccaca
     4201 ccgttgttgg aggcggcatc cagttcgtgg atgttgtagg atcgctgatc gttgaatgat
     4261 ttgcaggatt cacattggtt gcaggcttcg aattcttcgg tgagatgttc gcaattgatt
     4321 gttttggcaa aaatgcgggc acaggtggtt ttacccacac cgcgcggacc gcagaaaaga
     4381 taagcgtgag ccagtttttt gcttttaatc gcatttttca gggtggtagt gagcgcatcc
     4441 tgccccacta ccgagcgaaa ggtggaaggg cggtatttaa gagccgaaac aacgtatgaa
     4501 tccatctgta gttacttttc tggaggttag aaacgaatta caaatatagt tttttttatt
     4561 gaaatgacaa acaggctttc gggagctgaa ggtaatatcc cgaaacaaaa attattcgtt
     4621 cctgtaatct atcactgccg gagcagaaga ggcatagctg ccgaatatac ctatgccgtt
     4681 tgcgatgttt gtatagatct gcacaggttc tgtaaatgga tttccgcttt gattaccggc
     4741 tgcagccgac gatctggcat acatgtagta atcgtgggaa atttcctgca ggtaaatgta
     4801 gtaggtgctt ctcatttctt tccggaattg agaatcgtag cctttgtagt ctttttgact
     4861 tatccggatt gaattcgaaa atttcaaatc gtattctttc ccgttgaata aatcatctgt
     4921 aaaagtatcg aaaaaataat tgttgtctga gaattcaaac aggcttccga tgtcgttggt
     4981 ctgctgattt ccaaatacaa tgtcttcgaa atcaaataaa tacgattcat tcacctcatt
     5041 ttcatagtaa gtacgttttc ggactaccag ccggtagtag ttttttttgt taccgtcatc
     5101 acgcaatttt accctaaacc ggatgttttt gtacgtgtat tccgaaagcg tatcttgccg
     5161 gttggatccg ttgtattcgc ccgtgtagcc tatgtagtat tgcttctctg tcactgatga
     5221 tgtgtccacg ctcaggatga cgggctgcgg tggaatgacg gtggtggctt ttactgattc
     5281 aaatcccgat gcaactgctt ctattctgat ttcttcgccc ggcttcggtc tgtatgcagc
     5341 ccgataaatg cctgctccgg tatgtgtaag tttctctttg aaggtattat tcacgtacag
     5401 actgaaatcg gcgttcgtca ctttgttgta ttcatatcca ccgtagcctc cgcttccttc
     5461 gtccagaaaa aacctggatt cggtcaatac agcctttatc gccgagtcgg gtgtgacaaa
     5521 actgctcact accagcatcg gattcatgat ttctccccgg aattttatgg ttttctcgca
     5581 tccggcagtc agcaatgcca ccatcaaaaa taccatgtat atgtgtctga ttttcatttt
     5641 attaaaattt atagctgtaa ctaacagatg ggatgatggg gaacagggtt acctgtttga
     5701 gcacctggct gctctcgtaa agtccggtgc gcggattgta gctgccctcg gacgactgat
     5761 agaccatgaa cgggttgtac tgattgtaca cattgtagat gctgatgttc caggtgcgga
     5821 ttccccgttt tttctttttg tgaaaattca ctcccagatc catccggtgg tagttgttgt
     5881 agcggtagtt gtttcgggaa ctgatgtagg tttcagggcc cctgaaggtg tatccctgat
     5941 ttgtttcggg gatgacagta ccctgatagg tttgcatgcc gagtgtggct gtgttgccgg
     6001 tagcaaacac ccaattgccc gaaatgtcaa ttttttcgga gaatttatgc atcaccgtga
     6061 tgctcaggtc gtggcggcgg tcgtatttgg ccggaaatac ttttccgtag ttcaactcct
     6121 gtcccgggcg gtcgaagagc cgttcggttt tggaccaggt atagccaagc catccggtag
     6181 tgtttccgaa tgaacgctgg gccagaaatt ccactccata agaccatccg cgccccatca
     6241 ccactttttc ttcccagccg gacgattcgc ccataaagga tgcgccatcc ttgtattcaa
     6301 tcaggttgtt catggacttg tagtagcctt cgagcgagaa atcgagcagg tttttcaggt
     6361 tgtagaatat cccgaaggca tactgatcgg atttcatcgg ttcaataaac cgtgtggctg
     6421 gtacccacag gtcggtaggc atgctgatgc tgttgttgga aagcaggtga atgtactgat
     6481 tcatgtgagc gtatccggct ttgaccgaaa gttgttcgtt tatcagcatc cgcattccga
     6541 ggcggggttc tatggaaaaa taattcttct gctgcacgtt gaacgtggaa aaatggatgc
     6601 ctgcgttgag tttgaatctg tcggtgagtg tgatgttgtc ctccagatag gtcatggttt
     6661 cgtgtgcata gatatttcct ttgccgatta cggtatccat cgtttcgttt ttatcgttgt
     6721 aattattgaa aaaatagaaa gtggaaacat ccggcctgaa agtgtggtaa gtatagttaa
     6781 cgccgaattt aatatcgtgt gctgtatttg gcgcatagtc gaaatcagct ttcagcgata
     6841 catcttcaat ccccgagcgg tatcccaccc tgaactcttc ggtgcgtgtt tttggaggat
     6901 tgatggattt atcggtactt tcggagaggt aacccacgtt catattagac cggtagcggg
     6961 tgtatgcggc agttgtgttc atgaataact tgttggtaac cacgtgattc catcgcagag
     7021 cagcgatggc gtttccccag tcccagttca gtttcatcca gtcgcttgta tttctgtcgc
     7081 cgtacgacgt gctgctggtg cgatacctgg cgtatatggc atcatcgccc aggtaagcac
     7141 tcagatagag tttgtctttt tccgaaaatt tgtgcgtcag tttggcgttg agatcgtaga
     7201 agtagtatcc ggccgtggtg ttacctgttt cgtcgccaga gttttctttt gctgcccttt
     7261 tgatgatggg ctggctcagg aaatcgatgt aagttctgcg tgcagagatg ttgaaggtgg
     7321 ttttttcctt tattatcggg ccttccaggt tgaattttga cgagataagc ccaacgctca
     7381 cattgccgtg aaagttttta tcgtcgccat ctttcattct cacatctacc accgatgaga
     7441 gccttccgcc gaaacgtgcc ggaaaacttc ccttgtagag ggtaacattc tttacggcat
     7501 cggtattgaa tgccgaaaaa aagccgaaca tgtggttgac gttgtaaatc ggaattccat
     7561 ccaggagcat caggttttga tcaggtccgc ctccgcgcac atacaatcct gccgaacctt
     7621 cggtgccgga ttgtactccc ggcagcagtt gcagggcttt gatcaggtcg ttttcgccga
     7681 agagtgtcgg gatgtttttg atttgctcta tgggcacttc gatggcgctc atctgggtgc
     7741 cctgaacgcc catttcgttg cgcctgccca ctaccgttac ttcgcggagg gctatgctgg
     7801 catccatttt tacgttaatc accgtgtctt ttgtaagtct gaaattactt ttttcagtag
     7861 tgtatcctac gtaactgtaa ttcaaatcca cttcgccggc attcagtgaa atggaatagt
     7921 ggccgtaatt atttgttacg gtcccttttt ttgatatttc atcaaagaca gaggcgctga
     7981 tgagtgtttc accgttcgat ttgtcggtga tgtacccgct gatggtatat cgctgtgcat
     8041 gcaatgtaat tgaccacacg acgagtgaaa tgaggattat tatttttcgt atctgcattt
     8101 tttttcgttc attatgtaat gcaaatgtaa gaaaattccg gcaactcatc ttactgtaaa
     8161 gtattgttct gttcatataa ctaattttaa acaacctgaa tttcccgatt gtttattgtt
     8221 tcaacaacct gtattctatt taaatatttt ccggtaaatt ttatataatt gctttaaaaa
     8281 cgtcatttgt cataatttcg cctctacgag gctttatatt ctgatacata ttatttctat
     8341 cctaattttt tcctctacgt ggcttaagtc attagcacca gcggtgggac aatctcgtag
     8401 aaaatagatt acacccatca acaaaagttc ttcggagcga cattattttt tcggacaaaa
     8461 acttttcaaa aaacataatt ctttacctga tcccggcaat gtgaagtttt tttttgaaaa
     8521 aaaactcctg catttttttt gcaggagttt gtttggagtg aattactgat tgggagccgg
     8581 cgcttcttga ggagctgcag gtggtgttgc gttctgaatt atattggctt ttttctgtgc
     8641 tttctcggca tttttttgtg ctttgtcagc gtttttctgt gctttttcta cttctttttg
     8701 tagttttttg gcctctttct gtgctttagc ttcggcttta gcggcttctt tattggcttt
     8761 gtcgatttgt ttctgttgag cagcggcttc tttggctgct tttttctctg ctgcagcttt
     8821 ttctttttgt gctttagctt ctgcttttgc agcctgagct ttggctttat ctatgctttt
     8881 ctgtgatttt tcggcttctt tagcggcttt tgctgcagct ttttctttct ctttttgtgc
     8941 tttggcttct gctttggcag tattggcgtt ggctttatca attagttttt gtgctttttc
     9001 agcttctttg gctgcttttt gtgctgcttt gtctgttttt tcctgcgctt tggcgttggc
     9061 agcgtctatt ttctgttgag ctttttctgc attcacgcgt gctttttcgg cttcctgagc
     9121 agctttttcg tttaccttgt caatttcttt ttgagctgtc tgtgcttttt cctgggcttt
     9181 ttcagattcc tgctgggctt tatctaccac tttttgtgct ttttcacttt ctttttgtgc
     9241 tttttcggct tctttctgaa gtctttctgc ttcatccagt ttctgttttt gttcttttaa
     9301 gtaggcgtcc tgttcggcct taatctgttt gttgaaagct tcgcggtgtt ccagattttt
     9361 ggtgtaggga ttttcgccct tcatttcaat ttcaatttgt tttgcattgt tgaaatctac
     9421 ctcactgaat ttttggggca gtgtggggct gttgaaccat tgtccgtttt cttcatagat
     9481 gtaaacgttt tttttcaggt caaaatacat attctgatcg ggcagaaata cgtgtgtggt
     9541 tttggcattg taaccgccgg caggtgccca gggtggagtg gtaacggcat ttgcaggtgt
     9601 tgtggtgatg attgcgaatg tcaaaacgac aagtgcagca acgatttttt tagttttcat
     9661 gtagtggttt ttttatgttt gattttttaa aaaatattca ggtcgagtat ccaattcagt
     9721 gccaaaagta taaataatca ttcaaaaaaa caactcctga agttgttgat tatcaggagt
     9781 tgttttcaga agtgactgta tatggcctga aaatacggtg tatccttatt ttacttacgt
     9841 cttattggct tttcggctac cggtggtttt tgttttgttt gtaatatttt tttaccattt
     9901 tcttattggc ccggtggtat tttttatctg ctttgtacat ggctttgctg taagtccgtt
     9961 gatgtttttc cattcgtttg tagtagtcgt gctgtgcttt ccggttccag tatttagccc
    10021 tgtgattgct gttgtaggcg tatggccgat gattgtttat caccagttgc acctgtggaa
    10081 ctacactcag gtttatattt ctgtacataa ccggcagcgc agcacttgta ctccagtttc
    10141 tgccgttcat gtaaatatac acgccgcgat gaaggtcgta atagaaattt tgctgtggaa
    10201 aataaatgtg ggtgtttttt gctctgtatc cgttggcagg tgcccaatac ggaggcgctg
    10261 ctgcggattc tgccggtatt gctattgtca gcgcgaacat ggctgcaaat gcaacgatca
    10321 attcctttgt tttcataatc ttaagtttta aatagttact gaattagcta tcttactttc
    10381 gatatttcaa tatctgtgcc aaaaaaacac cctttgactt ggaattaagt tcaaagggtg
    10441 tcattctgga aataaaaata ctctgttaca gcaggtattg ttcgctgatg gatttgtgtt
    10501 cctgcaccct tcggatggta tcggcaaaca tatcggcaat gctcaggatt tttactttcg
    10561 cgcattttgt gcagtcgaat ggaatggaat cggtgaatgc aatttcagtc agtttggagt
    10621 tggtgatgtt atcgctggca attccggaca tcacaccgtg tgaaacgatg gcgcgcacac
    10681 tgagtgctcc attgtcaatc atcagattgg ctgccttgca gagggtgccg gcggtatcta
    10741 ccatgtcgtc aactataatc acatttttgc cctccacatc acctattatt ctcatttcac
    10801 ctaccacatt ggctttttcg cgtgttttgt ggcagagtac catcggcact tccaggtgtt
    10861 tggagtaaga tcctgcccgt ttggagcctc ccacgtcggg agaggcaatt accagatttt
    10921 caagagccag cgacttgatg taaggaataa aaatggtgga tgcatacagg tggtcaaccg
    10981 ggacgttgaa aaatccctga atctggtcag catgaagatc catggtgata acccggtcca
    11041 cgcccgctac ggtgagcatg tcagctataa gttttgctcc gatggatacg cgtggtttgt
    11101 ctttccggtc ctgacgcgcc cacccgaaat acggaataac tgcgatgacc ttgtaggccg
    11161 aagcccgttt ggcagcgtcg atcatgagca gaagttccat cagattgtcc gagttgggga
    11221 aagtggactg aaccaggtag atgtactgtc cgcggatgga ttcttcgtaa gagaccgaga
    11281 attcaccatc ggaaaagtgt tcgatattca tttttccaag tggccaattt aaacttttac
    11341 agattttttc ggccagataa cggctttttg tgcccgaaaa tactttgaat aaagtggaat
    11401 cttctgacat aaattcggtg agttttttgg atgagtaatt ttttgcaaaa ttacagaaat
    11461 ttagataaaa tccgaagttt tttttgaaac aaatcaaatc aatttcttaa cgtatggaaa
    11521 atcaaataga taaccgtatt ttttataaaa tgtgaatgag gtaatgattc aatgcaaacg
    11581 tttgaaacct ttttgaatgt cttttttaac tttactccga gacagataat acattatgaa
    11641 gtaattttga atttacaaac ttttatcgaa aacaattctt aagggtaaag tcatgaaaat
    11701 acacgtaaat ataccttatt ttactcgctg gggacaacgc attctggttt caggaaatgt
    11761 gaaagaactc ggtgacggag attttagcaa agcgctgccg ctcagcctta acagccacga
    11821 aaactggtac ggagaatttg agtgcaaccc cccagccacc gaaccgttga aatacaagta
    11881 cctccttttc gacgaaaaca caggaaaata taccgaagaa tggggagata accgcacagt
    11941 ggagctccaa aaaaacacag acgattacta ctgctttgac agctggaatg tggccggaag
    12001 cgtcgaaaat gtgttcatga cctcaccatt ccggaaagtt ttactcaaac ccggcatagc
    12061 agctcttgca aaagcaaaac agccggctaa atatacgcat aaattcattg ttaaagcgcc
    12121 gctgctgaaa ccaaaccagg cactttgcat tataggaaac tgcaagcagc tgcgcaactg
    12181 gacagtatcc gatccggcga tgatgaatct tcgtgatgat gaatgggaac ttaaaattga
    12241 tttaagcaag gccaccaccg aaattcatta taaatacggc gtttatgacg ctgaagcagg
    12301 tcattttctc tttttcgaac agggccccga cagaatagct gctgtaattc aaaattcggt
    12361 tgtgcagaca aacgatggct ttattcgggt tgataatcag tcctggcacg gggcaggact
    12421 tggattgcca gtgttcagta ttcgtacccg caaaagtttt ggagtgggcg attttgccga
    12481 tctgaaacta ctcgttgact ggtctgaaaa aatcgggatg aaactcattc aactattacc
    12541 cctgaatgac actaccggaa cacataccga acttgacgtg ctgccctacg cagccatatc
    12601 agcttttgcg ctgaatccgc tttttctaaa tcttcc
//
LOCUS       DAORKP010000057        12479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_487_length_12479_cov_8.517305, whole genome shotgun sequence.
ACCESSION   DAORKP010000057 DAORKP010000000
VERSION     DAORKP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_12479_cov_8.517305"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLN75_04675"
     CDS             <1..774
                     /locus_tag="PLN75_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG03444.1"
                     /translation="AGNIDSNTSLISQRPFRSPHHTISDVALVGGGTFPQPGEISLAH
                     NGVLFLDELPEFKRTVLEVMRQPLEDRKICISRAKFAIEYPASFMLVASMNPCPCGYY
                     NHPTRECVCAPGLVQKYLSRISGPLLDRIDIHIEIIPVPFEKMSELKESESSNEVRKR
                     VVKAREIQELRFRDVDGVYCNAQMSSKLLRTYAQPDKEGSELLKNAMNRLNLSARAYD
                     RILKVSRTIADLDDCENILPQHIAEAINYRNLDREGWAG"
     gene            complement(890..2089)
                     /locus_tag="PLN75_04680"
     CDS             complement(890..2089)
                     /locus_tag="PLN75_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG03445.1"
                     /translation="MKKHYSLILLAVLLSAATMSYANQTVKLRVQVPAQTVVCYATGG
                     FNGWNTSATPMTKVSDSPKIFEADISVPDNVDAGYGFKFLAGPDWKYEQGDPSADFTY
                     GAVGWTGAVVNSFKQYFTPMTPRDIKVNVLVPSTTLFCYITGTFANWEQPTKQMTFVS
                     QDQDGKVYTYTIPNIDPQLLEFKFASGPSWAYEQTQGNFTYLGNGGDNNEVSVVCSGF
                     KAIYDPVNVGDITVNITSVPAGTDSVYLVGSFKNGWKLEEAIPATKNNDGSFTAVITQ
                     VADVEFKCWNRRDWAYEECQPDGSNIPNRQYKFKDTPVANITIAKWKKMATGIDELKS
                     DFTYPVSVSDRKVTVDGVVSGVSLYNISGQLIQNFKGNGKFVSRPLTSGVYILQVDNT
                     ERKVMVD"
     gene            complement(2155..3645)
                     /locus_tag="PLN75_04685"
     CDS             complement(2155..3645)
                     /locus_tag="PLN75_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRG03446.1"
                     /translation="MKKIIIYLYLGTFIFIFAGCAGCKGTNNPKPPDTGNPGTKDVAT
                     YITTADKTMLFSSVAKSFGSGINMNIEKTLTLKPTQTFQTIDGFGAAITGSSCYNLLK
                     MNKADRTKLLEETFSVDKGMGYSYVRVAIGCSDFSLDEYTCCDEPGIENFAMHPYDVR
                     DIYPILKEILAINPKLKIMGSPWTPPRWMKVNNLTDLQPYNSWTSGQLNPKYYQDYAT
                     YFVKWIQAMEKEGFPIESVTIQNEPLNRGNSASLYMTWQEQRDFIKTALGPKFADANL
                     KTKIIVFDHNFNYDNIPDQQGYPLRIYEDADAAKYIDGAAYHAYGGSSTELTRVHNSN
                     PNKNLYFTEMSIGTWNYTFDGDLMWTMKEIGIGALNRFSKAVIVWNFMLDDKRGPNRP
                     GGCTTCFGAVDINSSDYKTLDRKSHYYFIGHMSKVLHSGSTRIGTSGYMPAGVIATAV
                     VNPNGTYGIVLQNDNSTSVQMTIDDGNHHFDVTLPAKSLTSCNWKK"
     gene            complement(3656..5059)
                     /locus_tag="PLN75_04690"
     CDS             complement(3656..5059)
                     /locus_tag="PLN75_04690"
                     /inference="COORDINATES: protein motif:HMM:NF028474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HRG03447.1"
                     /translation="MKKLHYIISLAIIFIASSCQETEFRTHYPESTPTLTASVTENSI
                     QYGDSITLLAEVSDPNPLSTLEVKIAVSNKLIISESIRTKGTSASIQKRYHIPFGAGM
                     PDKEKVKVYLTAINTEGFKTEQVIETTIGNRPPMPKLYLIPATITAGLSTKELIVTDE
                     SNLIYSVSGIEFPNTFNCYIATKKTAFGRVDWSGIVFGMRNNEITIIKQGEAPISVQD
                     ESLVNISSCSFDAINFSLQVTGKLLEPLTHMNVAADLTLSPASLLGADASKFRGATIY
                     FGKNVEVTFEGIANLTNGLSPDYFEVTGTNKAKFLGETGMYKAYYHIAGDYLYIEPMP
                     TTALPDALWICGTGIGRPSQPYATTTSWNWNTPLDYVPCRKISNGVYQATVYLENEDN
                     GEGTGFGTCNFKFFHYRGWDHGEESSMQYTVNSPLKKSSEAGNVGNWRGNDTTFKGVY
                     RITLDMNTKITHVEKLN"
     gene            complement(5100..6614)
                     /locus_tag="PLN75_04695"
     CDS             complement(5100..6614)
                     /locus_tag="PLN75_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03448.1"
                     /translation="MKNIIIKSGLGLFIILFTACNLDYDPVSQLSERTLGTKDTTGTE
                     IKYKDREAIQAVYKSMYQRMTGQQEHWYLDYLLFAECRSDNAYAGTTGAEVVPVETNN
                     LDASNSVIARDWNRYLEDIALANSIISNIDLVPDQTLTGAERNQWKAEAKIFRAMMMY
                     DMARWFGNFPVITREAGKITAENIKDVYPLYFPKANTPAEAYAQIISDLRSSLEFAPA
                     NNNADKSILSKTVARALLTKVYADRIVQNPDSVIYFANQVAADGITLVGNYSDLFGLN
                     ASGTDAKARNTSESILELQYFTGSGNWVTWMFGRDLLDFEFYFTWAKWVTPSRDLIKA
                     FDDAGDTKRKNESIVYYQTTWSNYYDSKSYPFMYKCRSNVNSIIKLRGADILLLKAEA
                     LAHKNQLQEAAQVVNTVRKRVSLPDLNASSTSSKENLLNAILLERRLELAFEGQRWFD
                     LIRHNKLMEVMNNVNQRDAGRLKQARAFTQESELLPIPQPVLDENTNLVQNPGY"
     gene            complement(6651..9635)
                     /locus_tag="PLN75_04700"
     CDS             complement(6651..9635)
                     /locus_tag="PLN75_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03449.1"
                     /translation="MKTHKKHLFSFVILLLMFFSQQSTAWGQSGGNYQGTVTDTSGET
                     LIGVSVLIKGTTTGVVTNIDGQYNIAARQGDVLVFSYVGMVTRELPLGSQLVNNVVLT
                     EEAKGLEEVVVIGYGVAKRKDVTTAVSSVSTEDLDMRPITSAAQAIQGKAAGVQVIQP
                     NGRPGAGMVIRVRGTTSMNASNDPLYVVDGVPMNNIDFLSANDIESMQIMKDASSAAI
                     YGSRGANGVVMITTKATARKDRSAISFSAYTGITNISRKIESLNLAEYKEYLTDLKSS
                     VVLPDGLTDQTNWFDETFTTGIRHNYQLSASGASNKVNYFISGGYTDETGIINTTAFK
                     RFNFRSSVESEMYKWLTLSANISYSNNISKGDIISGTGANRGGVILSVINTPTYAPVW
                     DTENPKHYYNRFYGVSNITHPLENLARTKNNKNDTHRLLATGKSVITFIPELKLSSSI
                     TADMNYWNYTSFLDPVSTSYGRGQFGEAQDNRSFTKVMMLDNILTYNKKINLNNFEAM
                     AGTSYTVSDWSQSYQFGSHFANDLIQTLNAANKISPSNGSTGSQWAIMSYLGRLSYNY
                     DSKYLATINMRADGSSKLAPSGRWGYFPSVSAAWRISSEPFMANFNWMNDLKLRAGWG
                     QTGNQDGLGDYAYLERYDFNRIEWWKPGQGDAVPTYSQVSLSSSDLTWETTTQTNIGL
                     DATMFQNRLTFNIDYYFKRTRDMLMWVTLPAGSAAVSSIQRNEGEMTNKGIEFSVSSK
                     NLTGDFKWNTDFNISHNKNMLVSLDFKQVYYDARVTDILSDFAVKNMPGRPLGGFWGY
                     ESEGVDPETGELMYVDKNNDGFINASDRGYIGDPNPDFTYGMTNYFSFKNFNLSVLLQ
                     GTYGNDIFNVSRIESEGMYDAKNQSKRILERWRRPGMVSTIPKAGYDMKISSYFVEDG
                     SYLRIKDITLSYDFSMNWMKKLGITRLQPYATVTNLFTFTKYLGFDPEVNQWGNSGNV
                     QGMDYGTYPQTRSFIFGVNVDI"
     gene            complement(9935..11557)
                     /locus_tag="PLN75_04705"
     CDS             complement(9935..11557)
                     /locus_tag="PLN75_04705"
                     /inference="COORDINATES: protein motif:HMM:NF039744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HRG03450.1"
                     /translation="MNFSITIAENHTNDSLILVYDQLLENYETQINKRQQRIDSLERI
                     SVKKNLSLIEQYNHNLQLYKEFRPYISDSAIYYQKKSISLAEKIGDKNLLSDSKLQLS
                     FFLASIGLYKESVDILNEIQEADLTPSLLIDYYNGMRHTYGELSFYGKDADLSNRYRQ
                     VSNTYTDKLRNMLQKEDDNLLDILESDYRNNRDFKMSLEINDLRLNSTTENSQKYALL
                     AFLRSLTYKESGDIYSQQKWLLKSAIADLKLGITDNASSWELANIMFKMGDIIRAHRY
                     INYSVNNSNIFNARLRFTQIAEVQSIINNAYMIDKAEQEKKLRTLLIFISILSFLLLA
                     SVVSILLQNRRISKANARTLKANDELNRLNWELNRINDEISNTNAELSESNQVKEEYI
                     GHFLSLCSLYIDKMDAYRKTIRKKLLNGQQIEVIEEAKSSDFMDNELKDFYRNFDRSF
                     LQLYPDFVHEFNNLLLDEEKILLKKDELLNTELRIFALIRLGIDNSAKIAELLHYSAN
                     TIYNYRAKIKNKARISRNDFETRVKKIGTFTK"
     gene            11889..12191
                     /locus_tag="PLN75_04710"
     CDS             11889..12191
                     /locus_tag="PLN75_04710"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRG03451.1"
                     /translation="MSQTVKHEPHTNLCSADNNWDFWSSLPEALQITSTMSERGIPAS
                     YRLIHSLVSHTYTFIYSQSQLLSVKFHLVNQQGIKNLTDAEAAELVDRDRKSHQRD"
     gene            12252..12386
                     /locus_tag="PLN75_04715"
     CDS             12252..12386
                     /locus_tag="PLN75_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03452.1"
                     /translation="MNDEQKKVFLENTARNMEGVEEFIKIRHIGKLLQSRSRLWQGCG
                     "
BASE COUNT         3338 a         2575 c         2647 g         3919 t
ORIGIN      
        1 gccggaaata ttgatagcaa tacatcgctt atctcacagc ggccattccg gagtccgcac
       61 cataccattt ctgatgtagc actcgtaggt ggcggcactt tcccccagcc gggtgaaata
      121 tcgctggcac acaacggagt gctttttctt gacgaactac cggaattcaa acgaacggtg
      181 ctcgaagtga tgcgtcaacc gctggaagac cggaaaatat gtatttccag ggcaaaattt
      241 gccattgaat atccggccag ttttatgctt gtagcttcta tgaatccttg cccgtgcggc
      301 tattacaatc atcctacccg cgaatgcgta tgtgcacccg ggcttgttca gaaatacctg
      361 agccggattt cgggtccttt gctcgaccgc attgatatcc acatcgaaat cattcccgtt
      421 ccgttcgaaa aaatgtcgga actgaaagaa tccgaaagca gcaacgaagt acgaaaaaga
      481 gtggtgaaag cgcgcgaaat acaggaactg cgattcaggg atgttgacgg ggtgtattgt
      541 aatgcacaga tgtcgtcgaa gctgcttcgt acgtatgctc aacccgacaa ggaaggcagc
      601 gaactgctca aaaatgccat gaacaggctc aatctttctg cccgggctta cgatcgaatc
      661 ctgaaggtat cgcgtaccat tgccgatctg gacgactgcg aaaatatact tcctcagcac
      721 attgccgaag ctatcaatta tcgtaatctg gacagagaag ggtgggcggg ataaaaaaat
      781 ctcagcagat ttgattgctg atattcagtt gctttgaata aagctgaaat aaaaaaaaga
      841 ccgtaagcct gatggcttac ggtcttttct ggttataaaa aaagcagaat taatcaacca
      901 tcacttttct ttcagtattg tcaacctgaa gaatataaac tccgcttgtc agaggacggc
      961 tcacaaattt tccgtttcct ttgaaattct gaataagttg tccgctgata ttgtaaagac
     1021 ttacacctga aaccactcca tcaacagtta ctttcctgtc agaaacagat accggatatg
     1081 taaaatcgga tttcaattcg tcgattccgg tagccatctt tttccatttt gcaatcgtaa
     1141 tattcgctac aggagtatct ttgaacttgt attgacgatt agggatattg ctgccgtcag
     1201 gctggcattc ttcataagcc cagtctctgc ggttccagca tttgaattcc acatcagcta
     1261 cctgagtgat tacggcagtg aaagaaccgt cgttgttttt agttgctggg atagcttctt
     1321 ccagtttcca tccgttcttg aaactgccta ccagatacac actgtctgtt cctgccggaa
     1381 ctgatgtgat attcacggtg atatctccga cgtttacagg atcgtaaatt gctttgaatc
     1441 cggagcaaac cacgcttact tcattgttgt cacctccgtt gcccaaatag gtaaagttac
     1501 cctgagtctg ttcataagcc cagcttggac ccgatgcaaa tttgaattca agcagctgag
     1561 gatcgatatt tggaatagtg taagtataca ctttgccatc ctgatcttga cttacgaagg
     1621 tcatttgttt tgtaggttgt tcccaattgg caaatgttcc ggtgatatag cagaataatg
     1681 tagtagaagg aacaagtaca ttcactttga tatcacgcgg tgtcatcggt gtgaagtatt
     1741 gtttaaatga attgaccaca gctccggtcc aacccacagc tccgtaagta aagtcggctg
     1801 acggatcacc ttgttcgtat ttccagtcgg gtccggccag gaatttgaat ccatatcctg
     1861 catccacatt gtcgggtaca gaaatatctg cttcaaagat tttcggagag tcagaaactt
     1921 ttgtcatcgg agtggcagat gtattccatc cgttaaatcc tcctgtggca tagcatacaa
     1981 ctgtttgtgc cggtacctgt accctgagtt ttactgtctg attagcataa ctcattgttg
     2041 cagctgataa taacacagcc agcaaaatta aagaatagtg ttttttcata aattttttgt
     2101 ttttaaagat ttgtaataaa attattgatt atcagatgaa tataaatatc ttttttattt
     2161 cttccagtta caagaggtca gacttttagc cggtaaagtg acgtcaaaat ggtgattgcc
     2221 atcatcaata gtcatctgta cggaagtgga attgtcattt tgaagaacaa ttccgtaagt
     2281 tccgtttgga ttgaccacag cagtagcaat tacacctgca ggcatatatc ccgaagttcc
     2341 aatccgtgtt gaaccactgt gaagcacttt cgacatgtgt ccgataaagt aataatgtga
     2401 ttttctgtcg agcgttttat aatcggatga gttgatatcc acagctccga aacaggtggt
     2461 gcatccaccg ggacggttag gtccccgctt gtcatcgagc ataaagttcc acacaatcac
     2521 cgccttactg aatcgattca atgctccgat tccaatctct ttcatcgtcc acatcagatc
     2581 tccgtcgaaa gtgtagttcc aggttcctat tgacatttcg gtgaaataca gatttttatt
     2641 cggatttgag ttgtgaacac gtgtcaactc cgtactgctt cctccgtaag catggtacgc
     2701 agctccatca atgtatttgg ctgcatccgc atcttcgtag atgcgtaacg gatatccctg
     2761 ctgatccgga atattatcat aattgaaatt gtgatcgaat actataattt tagttttcag
     2821 atttgcatct gcaaatttag gccctaatgc tgttttaata aaatcgcgct gttcctgcca
     2881 ggtcatatag agcgatgctg agttacctct gttcagtggt tcattctgta tcgtaactga
     2941 ttcaattgga aaaccttctt tttccatcgc ctgtatccat tttacaaaat aggtggcata
     3001 atcctgatag tatttcggat ttaactgacc acttgtccat gagttatatg gttgaagatc
     3061 agtaagatta ttcactttca tccatcgggg aggtgtccaa ggcgaaccca taattttcag
     3121 ttttggattt atagccagaa tttccttcag aatggggtaa atatcacgca catcgtaagg
     3181 atgcatggca aaattttcaa ttcccggttc atcgcagcag gtgtactcat caagcgagaa
     3241 atccgaacat ccgatagcta cacgtacata actgtaaccc atacctttgt caacactgaa
     3301 ggtttcttcc agcagtttgg tccggtcggc tttattcatt ttcaacagat tatagcacga
     3361 agaaccggtg atagcagctc caaagccatc gatagtctga aaggtctgtg tgggcttaag
     3421 agtgagtgtt ttctcgatat tcatgtttat gccacttcca aaacttttag ctaccgaact
     3481 gaaaagcatt gtcttgtcgg cagtggtaat gtatgtagcc acatctttgg tgcccggatt
     3541 tcccgtgtcg ggtggcttgg ggttgttggt tcctttgcaa ccggcgcaac cggcaaaaat
     3601 gaaaatgaaa gtgccgagat ataaatatat tataattttt ttcattctga tagttttagt
     3661 tcagtttctc tacatgtgtg atttttgtat tcatatccag cgtgatgcga tacacacctt
     3721 tgaatgtagt atcattaccg cgccagttac ctacattgcc ggcttcacta ctttttttca
     3781 acggtgaatt tacagtgtat tgcatcgaag attcttcgcc gtgatcccat ccccgatagt
     3841 ggaagaactt gaaattgcag gtgccaaatc cggttccttc tccattgtct tcattttcga
     3901 gatatacggt tgcctgatac actccgttag atattttccg gcatggaaca taatccagcg
     3961 gtgtattcca gttccagctg gttgtagtgg cgtagggttg cgatgggcgt ccgattcctg
     4021 tcccgcaaat ccacaacgca tccggtaaag ccgtagttgg cattggttcg atatacagat
     4081 agtctcctgc gatgtgatag taggctttat acatgcctgt ttctccgagg aatttcgctt
     4141 tattggttcc ggtaacttca aaataatcgg gtgataatcc attggtcagg ttggcaattc
     4201 cctcgaaagt aacttccacg tttttaccga aatagatggt tgctcctctg aatttcgatg
     4261 catcagctcc caaaagagaa gcgggtgaga gagtaagatc ggcagcaaca ttcatgtgag
     4321 tcagtggttc aagcagtttt ccggttactt gcaacgaaaa gttgattgca tcaaaactgc
     4381 aggagctgat attaaccaac gattcgtcct gtacggaaat gggagcttca ccctgcttaa
     4441 tgattgtaat ctcgttgttt ctcattccaa atacaatacc cgaccagtcc actctgccga
     4501 aagctgtttt tttagtggca atgtaacagt tgaatgtatt cggaaattca attccactta
     4561 cagaatagat aaggttactt tcgtcggtca caatcagctc tttggtgctc agtccggcag
     4621 tgatcgtagc cggaatgaga taaagttttg gcattggcgg acgattccca atcgtagttt
     4681 caattacttg ctcggttttg aaaccttctg tattgatggc agttaagtaa acttttactt
     4741 tttctttgtc cggcattccg gcaccgaacg gaatgtgata tctcttttgg atactggcag
     4801 atgtaccttt cgtgcggatg gattccgaaa tgataagttt attgcttacc gctattttta
     4861 cttcaagagt tgaaagcgga ttcgggtcgc tcacttcggc caataatgta atggagtctc
     4921 catattgaat ggaattttca gtcacacttg ctgtgagagt gggtgtactt tccggatagt
     4981 gtgtcctgaa ttctgtctcc tgacacgaac ttgcgataaa aataatcgca agactgataa
     5041 tataatgtag ttttttcatt gtttgcttat ggaaaactgt tcataataaa gtctgacaat
     5101 taataacccg gattttgtac cagatttgtg ttttcatcca ataccggttg cggtatcggg
     5161 agtagttccg attcctgagt gaatgctctg gcttgcttca atctgcctgc atcacgctga
     5221 ttaacgttgt tcatcacttc catgagtttg ttatgtctga tcaaatcaaa ccaccgttgc
     5281 ccttcaaagg cgagttcaag tcttctttcc agtaaaattg cattaagcag gttttctttt
     5341 gacgaagtgc ttgaagcatt cagatcgggc aaactaaccc tttttcgaac ggtattaacc
     5401 acctgagctg cctcctgaag ttgattttta tgagccaatg cttctgcttt aagcaataat
     5461 atgtcagctc cgcgtaattt gatgatggaa tttacattgg atctgcattt gtacatgaaa
     5521 ggatagcttt tggaatcata ataattagac caggtagtct gataataaac aattgattcg
     5581 tttttacgtt tcgtgtcgcc ggcatcatca aatgccttga tcaaatcacg tgatggtgtt
     5641 acccattttg cccaggtgaa ataaaactcg aaatccagca aatcgcggcc aaacatccag
     5701 gttacccagt ttccgcttcc ggtgaaatac tgcaattcca gaatagattc ggaggtgttt
     5761 cgggcttttg catcggtacc ggatgcgttc agaccgaata aatcgctgta gttgccaaca
     5821 agagttatac cgtcagcagc cacctggttg gcaaaataga tgacgctgtc cggattttga
     5881 acaattctgt cggcatatac ttttgtcagc aatgctctgg caactgtttt tgacagaatg
     5941 gatttatcgg cattgttgtt agcaggcgca aactccagcg aactgcgtaa gtcgcttatg
     6001 atttgagcgt atgcttcagc cggagtattt gctttcggaa agtaaagggg atacacatct
     6061 ttgatgtttt cagcagtaat tttgcctgct tcgcgggtga taaccggaaa attgccgaac
     6121 catcgggcca tgtcatacat catcatggct ctgaatatct ttgcttctgc tttccattgg
     6181 tttctttcag ctcctgtcag agtttggtca ggcaccaggt caatattact gatgatgctg
     6241 ttggctaatg caatgtcttc cagatagcgg ttccagtcac gtgcaatgac gctgttgctc
     6301 gcatccaggt tgttggtttc tacaggtact acttcggcgc ctgttgttcc ggcataagca
     6361 ttgtccgaac gacattctgc aaaaagcagg taatcaaggt accagtgttc ctgttgcccg
     6421 gtcatacgtt gatacatact tttataaact gcctgaattg cttcacggtc tttgtacttt
     6481 atttcagttc cggtagtgtc ttttgttcca agagtccttt cggagagttg cgatacggga
     6541 tcgtaatcaa gattacaagc cgtaaagagg ataatgaata atccaagtcc tgattttata
     6601 attatattct tcatgatttt cgaagttgct ttttaaaaag ttggatagaa ttaaatatct
     6661 acattgactc caaagataaa ggatcttgtc tgtggataag ttccgtaatc cattccctgt
     6721 acatttcctg agtttcccca ttgatttact tcgggatcaa atccgaggta tttggtaaag
     6781 gtaaacagat ttgtaaccgt tgcatagggt tgaagcctgg taataccgag tttcttcatc
     6841 cagttcatgc tgaaatcata tgataaggta atgtctttga tgcgcaggta gctgccatct
     6901 tcaacaaaat aggatgaaat tttcatatcg tatccagctt tgggaatagt agaaaccatt
     6961 cccggtcttc tccatcgttc aagtattctt ttcgactgat ttttggcatc gtacatgcct
     7021 tccgattcaa ttctcgatac gttgaaaata tcatttccgt atgttccctg taaaagcaca
     7081 cttaaattga aatttttgaa tgaaaaataa tttgtcatac cataagtgaa atcgggattc
     7141 ggatctccaa tgtaaccacg gtcggatgca ttaatgaatc catcgttgtt tttgtcaaca
     7201 tacatcagct cgccggtttc cggatcaaca ccttccgatt cgtatcccca gaatcctccc
     7261 aatggtcttc caggcatgtt tttcaccgca aaatcggaaa ggatatccgt tacgcgggca
     7321 tcgtaataca cctgtttgaa gtcaaggctc accagcatat ttttattgtg cgagatgttg
     7381 aaatcggtat tccatttgaa atcaccggtg agatttttgg aacttacact gaattctatc
     7441 cctttgtttg tcatttctcc ttcgtttcgt tgaatggatg acacagcagc ggatccggcc
     7501 ggcaatgtta cccacataag catgtcacgg gttcttttga aatagtaatc gatattgaaa
     7561 gtgagcctgt tctgaaacat ggtagcatcc agccctatgt tggtttgagt agttgtttcc
     7621 caggtgaggt ctgatgagct taagctcacc tgactgtatg tgggaactgc atctccctga
     7681 cccggtttcc accattcaat gcggttaaag tcgtatcttt ccaggtaagc gtagtcgcca
     7741 agtccgtcct gatttcctgt ctggccccat ccggcacgga gtttcaggtc gttcatccag
     7801 ttgaaattgg ccatgaatgg ttctgaagat atacgccagg ctgctgatac agacggaaaa
     7861 tacccccatc gtccggaagg tgccagtttg ctggagccgt cggcccgcat attgatggtg
     7921 gccaggtatt tactgtcgta attgtaagat aagcgtccca ggtatgacat gatagcccat
     7981 tgcgaaccgg tagagccgtt tgaaggactt attttattcg ccgcattgag tgtctgaatc
     8041 aaatcgttgg caaagtgcga tccaaactga tagctctgcg accagtcaga aacagtgtat
     8101 gaggttcctg ccatcgcttc gaaattgttc agattgattt tcttgttgta ggttaaaata
     8161 ttgtcaagca tcatcacttt agtgaatgaa cggttgtcct gagcttcgcc gaattgtcct
     8221 cttccgtagc ttgttgagac cggatcaaga aacgatgtgt agttccagta attcatgtcg
     8281 gcagtgatgg atgaacttaa ttttaattcg ggaatgaatg tgattacgct ttttccggtt
     8341 gccagcaggc ggtgtgtgtc gttcttattg tttttggttc gtgccaggtt ttcgagcgga
     8401 tgagtaatgt tgcttacccc gtagaatctg ttgtaataat gttttgggtt ttcagtatcc
     8461 catactggtg cgtaggtggg tgtgtttatg acagataaaa tcacaccacc gcggttggct
     8521 ccggtaccgg atatgatatc tccttttgaa atgttgtttg aataagaaat gtttgcgctg
     8581 agagtaagcc atttatacat ttctgactca accgacgatc ggaaattgaa acgcttaaag
     8641 gctgtggtat tgatgatacc tgtctcatct gtataaccac ccgaaataaa atagttgacc
     8701 ttgttggatg cacctgaagc tgagagctga tagttgtgtc tgattcctgt ggtgaaagtt
     8761 tcgtcaaacc agttggtctg atcggttaat ccatccggca gcacaacgga agatttcagg
     8821 tcggtgaggt attctttata ttcggccaga ttgagcgatt cgatttttct ggaaatgttg
     8881 gttattccgg tgtatgcact gaatgaaata gctgagcggt cttttcgtgc tgttgctttg
     8941 gtcgtaatca tgactacacc gtttgcaccc cgtgaaccgt aaatggcagc ggacgaagca
     9001 tctttcatta tttgcatact ctcgatatcg ttcgccgaga ggaaatcgat gttgttcata
     9061 ggtactccgt caaccacata tagcggatcg ttactggcat tcatggaagt agtacctctg
     9121 acacgtatca ccatacctgc accgggccgt ccgttaggct gaattacctg aactccggca
     9181 gctttacctt gtatggcttg tgcagctgaa gtgatggggc gcatatccag gtcctctgtt
     9241 gatactgaag aaacagctgt ggttacgtct tttctttttg ctacaccgta accgatcact
     9301 acaacttctt ccagtccttt tgcttcttcg gtgagtacaa cgttgttaac taactgactt
     9361 ccaagaggta attcgcgggt aaccattcct acgtaagaaa atacgagcac atctccctgt
     9421 ctggcagcga tgttgtattg tccgtcgatg tttgttacta caccggtagt ggttcctttg
     9481 atcaatacgc tcacaccaat cagagtttct ccgctggtgt cagtcactgt tccctggtaa
     9541 ttaccgcctg actgacccca cgcagttgac tgttgggaga aaaacatcag cagtaaaatc
     9601 acgaaggaaa ataaatgctt tttgtgtgtt ttcatacgta tctgttattg tttttgtgtt
     9661 tctgttaaat acctgaaaat agctgacaaa ccggttttac ttaaagttta aaaaaaattg
     9721 atttcgaaag taatgacctt tgaccttttt ttataaaaca agattttctg tcaaaacgtt
     9781 taagcaaata tacagtatgt tgttacgaaa taatccgtta gaaaaagatt aaggggataa
     9841 ggagggagtg aatatgcaga atatcagatg tttgtattat ttttggggtg taaaaaagtg
     9901 gatgggaata tcaaatatcg gagcttttga ttgactattt tgtaaaagta ccgatttttt
     9961 tcactctcgt ttcgaaatcg tttctggata ttctggcttt gttttttatt ttagcgcggt
    10021 agttgtaaat cgtattggcc gagtaatgca gcagttcggc tattttagcg ctattgtcta
    10081 ttcccagcct gatcaaagca aaaatgcgta attccgtatt taataattca tcttttttta
    10141 gtaaaatttt ttcttcatcc agcaacagat tgttgaattc gtgaacaaaa tccggataaa
    10201 gctgaagaaa cgagcggtca aagtttctgt aaaaatcttt cagctcattg tccatgaagt
    10261 ctgatgattt agcttcctca atcacttcaa tttgctgtcc gttaagaagc tttttacgaa
    10321 ttgtttttcg gtatgcatcc attttgtcta tatacaacga acacagcgaa agaaaatgcc
    10381 cgatgtactc ttcttttacc tgattggatt ccgacagttc ggcatttgta ttacttattt
    10441 catcgtttat tctgttcagc tcccagttga gtctgttcag ttcatcattt gccttcaagg
    10501 ttcgggcgtt ggcttttgat atcctcctgt tttgaagcaa tatacttacc acagatgcca
    10561 aaagtagaaa agaaaggata ctaatgaaaa tgagtaaggt acgtaatttt ttttcctgct
    10621 cagctttgtc tatcatataa gcgttgttaa tgatagattg cacttcagct atttgtgtaa
    10681 atcttaatct ggcattgaat atattggagt tgtttacgga gtagttgata taacgatgtg
    10741 cccgaatgat atctcccatc ttaaacatga tgttagccag ctcccacgaa gaagcattgt
    10801 cggtgattcc gagtttcaga tcggcaatag ctgatttgag cagccatttt tgttgagaat
    10861 agatgtcacc gctttctttg taggtaagtg acctgagaaa tgcgagcaga gcatatttct
    10921 ggctgttttc agtagttgaa ttcaaccgta aatcgttaat ctccagcgac attttgaaat
    10981 ctctattatt ccggtaatca gattccagga tgtcaagcaa attatcgtcc tctttctgta
    11041 gcatattcct gagtttatcc gtatatgtat tagacacttg tctatatcga ttgctcagat
    11101 cggcatcttt tccataaaac gagagttcgc cgtatgtgtg tctcattccg ttgtaataat
    11161 ctattaataa cgatggtgtt aaatcagctt cctgtatctc attcagaata tccactgatt
    11221 ccttgtaaag gccaatagaa gcaagaaaaa acgatagctg tagtttggag tctgacaaca
    11281 ggttcttgtc gccgattttt tcggccagtg aaatactttt tttctggtaa taaattgccg
    11341 aatcagaaat ataaggacgg aattctttgt aaagttgtaa gttgtggttg tattgctcaa
    11401 taagactcag attctttttc accgaaatac gttccaaact gtcaatcctt tgttgccgtt
    11461 tgttgatttg tgtttcgtaa ttttcaagca attgatcgta tacgagtatc agcgagtcgt
    11521 ttgtatgatt ctcggcaatc gttattgaaa aattcacgaa aaaaagaatt agtgatatca
    11581 attgtttggt cataagtttt gaaaaaatat aaatatgttg ttaatgattg actgaatttt
    11641 acgaaaaaga taaaatgtca atttagcttc ttccatgtcg gctaaagtct gatttcctta
    11701 caggttacaa atataacaaa tgaaccgtaa caatcatcgt tcaacatgtt cttttgttgc
    11761 tgttttaaaa gcatttgatc gtttttatct gttaaatagc taaataatgt tctcttgtat
    11821 aattccacaa agctacaccg gacattgcac ccgttttttt catccgcgat ccgctgaaat
    11881 ttcccgatct gagccaaacc gtaaaacacg aaccgcatac caatctgtgc agtgccgata
    11941 ataactggga tttttggtcg agcctgcccg aagcgctcca gatcaccagc acgatgagcg
    12001 aaagaggcat tccggccagc tatcgactca tccatagttt agtgagtcat acctacacct
    12061 ttatctattc ccaaagccaa cttctaagcg taaaatttca cctggtgaat cagcaaggga
    12121 ttaaaaatct gaccgatgct gaagccgccg aactcgtgga cagagaccga aaaagtcatc
    12181 agcgcgattg actcgaagcc agactacaca gcgaaaatta ctacgagtaa ccgggaaaac
    12241 ttttacgcct catgaacgac gaacaaaaga aagtattttt ggaaaatacg gccagaaaca
    12301 tggaaggagt agaggaattt atcaaaatac gccatatcgg aaaattgctt caaagccgat
    12361 ccagactatg gcagggctgt ggctgatgct tgcggaatca catacgaaaa agctggaata
    12421 aaataaaaaa aacctgaaat ttattgcaac tgtttacatc tgaaagaaca gggaaaatc
//
LOCUS       DAORKP010000058        12363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_495_length_12363_cov_10.208889, whole genome shotgun sequence.
ACCESSION   DAORKP010000058 DAORKP010000000
VERSION     DAORKP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_12363_cov_10.208889"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2263)
                     /gene="rnr"
                     /locus_tag="PLN75_04720"
     CDS             complement(125..2263)
                     /gene="rnr"
                     /locus_tag="PLN75_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRG03453.1"
                     /translation="MRKRRKEQGSTRLRKNDLLRNITAIFTDNPQKTYNYKQISNQME
                     ITSDSQRAFINELLYQLRDEDLLTEISRGKFKLNSRRGYIIGRFDRQGVKSFLIPDDG
                     GEPVFIPERKTGTAMLNDKVKVFMYAHRKGKEPEGEVVEILQRSQDTFVGILEVSDNF
                     AFLNVQSKVLNSDILIPKNKLKDGKTGQKALVKIIEWKHTMKSPVGEVIDIFGDKGDN
                     DAEMHAILAEYGLPYKYPENVEAEAAKIDSGITPQEISKRLDMRDVTTFTIDPADAKD
                     FDDALSIRKLENGLWEVGVHIADVTHYVRPGTIIDKEGRERATSVYLVDRTVPMLPEH
                     LSNGICSLRPDEEKLTYSVVFQLNEYAEVKSYQIVKTVIRSNRRFTYEEAQNIIETGT
                     GDYKDEMLKLNELAKILREKRFRSGAIAFDREEVRFEIDEKGKPLSVYFKVQKDSNKL
                     IEEFMLLANKTVATHIGKPGKGQKAKTFVYRIHDTPDPEKLENFSAFIRKFGYNLKST
                     GKKTQISSAINKLLDDVMGKKEQNLIETLAVRSMAKAIYSTQNIGHYGLAFDYYSHFT
                     SPIRRYPDMMAHRLLEHYLNGGKSVNEKDTEELCEHCSNREQVAANAERASVKYKQVE
                     FMAEHLGQTFDGVISGITEWGIYVELAENKCEGMIPIRDLDDDYYVMDEKNYCLIGRR
                     YHKKYQLGDELTVKVAKANLDKKQLDFVLA"
     gene            2392..3282
                     /locus_tag="PLN75_04725"
     CDS             2392..3282
                     /locus_tag="PLN75_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRG03454.1"
                     /translation="MKKNIAFIVNPVSGTTSRWNVTELIRDNLDFSHFNECRIMNTER
                     KGHGRELSRQFADEGFDVVVAVGGDGTVNEVAGALINTDTALGVISTGSGNGLARHLK
                     IPLNIKQAIEHLNFAEAIKMDYGLVNDEKTFFCTCGFGFDAYVSELFSKGTKRGWMGY
                     IEKMVTGYFNYEPQPYRLVGEDIDLKGNAFVITFANASQWGYNAYIAPNASIQDGMID
                     ISVITNVPIIAIPTIAFQLFTKTIQKDLLVTTLRTEEITLYRNKPGLFHLDGDPYELG
                     DKIHIKMMQEGLKVMVKKRF"
     gene            complement(3298..4338)
                     /locus_tag="PLN75_04730"
     CDS             complement(3298..4338)
                     /locus_tag="PLN75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HRG03455.1"
                     /translation="MKRFFFIFTILLPLSGMAQTITEGQSTLIYSLPKTEFVINATVE
                     IVTEIPGKFYQYSERFLATSDVITAEKKYYRLKKISMDTRTVSDAERTFTIIPSKKSL
                     ANQLTVNDEGILCGINVAPAKQVKEIRVERIEQKEDLESAKLLPLSEEYMLAGSVAKM
                     AEGAAKHIYRIRENRMDLLSGDVDNMPTDGASLKAMIAEMEAQEKELTELFTGKTTVQ
                     TLTQTVVYTPDSAVDDKIVFRFSSMQGVVDADDLSGVPYYLNIKYNPIKIETPEKPAK
                     KKDIEVFTVIPVTADVKFDDGNKILYENKLTLPQLGILVPIPFETMDKYSKAYVSPET
                     GRLLSVEQLPKK"
     gene            complement(4428..6773)
                     /gene="topA"
                     /locus_tag="PLN75_04735"
     CDS             complement(4428..6773)
                     /gene="topA"
                     /locus_tag="PLN75_04735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRG03456.1"
                     /translation="MSKNLVIVESPAKAKTIGKFLGNEFEVMSSMGHIRDLSEKGLGI
                     DFENNYAPQYEVSADKKKIVSELKKAAKSSETVWLASDEDREGEAIAWHLAEELKLKK
                     ENTRRIVFHEITKDAILHAVENPREIDINLVDAQQARRVLDRIVGFELSPVLWRKVRP
                     SLSAGRVQSVAVRLIVEREREINNFNPEASYRVSALFTSKDAAGTNVELKAELSQRFK
                     TKEEALAFLELCKSAKFTVDSIEKKPLKKSPAPPFTTSTLQQEASRKLGFSVTQTMVV
                     AQKLYESGKITYMRTDSVNLSALAIGTAKQEIIQMAGEKYVKIRQFSTKTKGAQEAHE
                     AIRPTYISSHTIEGTAQEKRLYDLIWKRTIASQMADAELEKTNIYINISGSNHQFIAS
                     GEVIIFDGFLKVYLESTDDEPDAETSGILPTMKKGESLTSNEILAQERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRNYVEKGDRQAIKRDFNVLKLKDGKISDKIK
                     SENTGAEKSKLFPTDIGLVVNDFLFEYFPNIMDYNFTANVEKEFDHIAEGKVKWTKSI
                     DNFYKKFHPVVESTLKNSERQVGERILGIDPKSGRQIAVKIGKFGPIAQIGKSEEEEK
                     PVFASLHRNQSIESITLEEALELFKLPREIGEFENKEMIAAIGRFGPYIRHDGKFYSL
                     PKTDDPLEISAERAVEIIEAKRESDKNKVIHIFGQNGEIQVLNGRFGAYIAFEKNNYK
                     IPKNTDVATLTEEQCREIITSQADTVGKKKKPFAGRRKKAQ"
     gene            complement(6897..7118)
                     /locus_tag="PLN75_04740"
     CDS             complement(6897..7118)
                     /locus_tag="PLN75_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRG03457.1"
                     /translation="MEKTAIIRGMSCSHCAARVSKALNKIDGVEAIVDLKSRTARINL
                     TKEVTDDVIKNAVDDVGYEVTQITAFSEK"
     gene            complement(7167..9440)
                     /locus_tag="PLN75_04745"
     CDS             complement(7167..9440)
                     /locus_tag="PLN75_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRG03458.1"
                     /translation="METKKFDVTGMTCSACVAHVEKSVRKLNGVEDVTVNLLTNSMSV
                     NYDQNSLSALNIIESVQDAGYDAYLKAETPSDNDLKVNHAENEQLAMKKRVVLSFIFL
                     IPLLYISMGNMLHFPLPAFLAGEKSALSFALTQLLLTVPVYIINRKFFSNGFRSLWKL
                     APTMDTLVAIGSSAALLYGIFAVFRIFNASLNNDSGIIHHYLHDLFFESGATILTLIT
                     LGKYFEARSKRQTSDALTKLINSAPQTASVIRDKKEIEIPVEEVVAGDLISIRPGEKI
                     PVDGIVESGFSSVDESALTGESIPVFKQTGDKVISASINKNGFLTFRATKVGNDTTLS
                     QIIHLLEEASASKAPISKLADRISGVFVPVVIVIAVLATFFWLISGYSAEFALSIGIS
                     VLIISCPCALGLATPVAIMVGTGKGAEHGILFKSAEALETGDKINTIVFDKTGTITEG
                     KPEVTDIESIHYMDEKELLRICASLEKYSEHPISAAITRKAEDEKLDLYPVTEFTSIP
                     GKGISGIINGEFYKVGNKAFISESIDIEEVEDFLFQLLQQGKTALFVADDENVLGIIG
                     VADVLKPTSREAVEMLKSMNMYTVLLTGDNQRTAFAIQDKIGFDSVIAEVMPQDKDKI
                     IQNLQAEGKKVAMVGDGINDAPALARSDLGFAIGSGTDVAIEAADVVLIKNDLVDVVT
                     GIRLSKSVIRNIKENLFWAFFYNILGIPLAAGVFYLSLGWKLSPMFAAAAMSLSSITV
                     VTNALRLKNFKPTKSKK"
     gene            10204..10809
                     /locus_tag="PLN75_04750"
     CDS             10204..10809
                     /locus_tag="PLN75_04750"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HRG03459.1"
                     /translation="MKRFGFLLVGIILWIGASAQVQADTTQVTIPDSVIEKYAATSEN
                     TREAILLQKLNPDQLLEIEKQRLDNERRRESNEIPMPMWALVLISTAPFVMVVFIVFF
                     TSRAKKEREKARYDLYLKSIEMGQPLPEKIFEEPEKKVSRLQNGLVWLGIGLALVIVG
                     ITTRNDGLLFGMVPGFIGLGILIAYLIEKPKDNSGSSAINE"
     gene            10802..11347
                     /locus_tag="PLN75_04755"
     CDS             10802..11347
                     /locus_tag="PLN75_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRG03460.1"
                     /translation="MNNKAEDLLWIAQVVVRDDRRAFDRLVCKYQSPLRRFLLNLTLG
                     DSALSDDLAQETFIKAYLNIRSFQGVSAFSTWLYRIAYNIYYDSVRSNKRYEDLNEPE
                     IDKKTSVSIEFSAEKSDIYTALQLLRKEEKAAILLCYMEEMTHKEIAGIMKLPLGTVK
                     THILKGKEKISNFLIHQGYGK"
     gene            11337..11651
                     /locus_tag="PLN75_04760"
     CDS             11337..11651
                     /locus_tag="PLN75_04760"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HRG03461.1"
                     /translation="MENKEDVLMRDFFGKNREEIPDNGFSDLVIRRLPGKKRKTNWIV
                     PVFALIGTVISLFVIDIHEVVLRAVELVAGIPFVYLLAGFMIFPITILFLYYFCEKER
                     AF"
     gene            11838..12329
                     /locus_tag="PLN75_04765"
     CDS             11838..12329
                     /locus_tag="PLN75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03462.1"
                     /translation="MKRSCFLLLLFAGVLSCNHHAGKNNSETIPETDSVVQLAPIDTL
                     ALTLDALRNKPLQTGYKMIGDVRTAILTYADFRMGDAALYIFKNETGDEIAFSGNDTK
                     VPLTVKSMQPTIENGGKNPNPLFLNKRFRVVWRRIQLNHKPQNEMELYYQEYDEIIYL
                     KEM"
BASE COUNT         3392 a         2499 c         2575 g         3897 t
ORIGIN      
        1 cctgtaacct tctgatccgt agtcagatac tctattcagt tgagccacgg ggccaattcc
       61 taataattta ggactgcaaa gataaggcaa aaatttaatt tatcaaatga aaaacggaaa
      121 ttttttatgc cagaacaaaa tcaagctgtt ttttgtccag atttgctttg gcaactttca
      181 ccgtcagttc atcaccaagc tggtactttt tgtggtatcg gcggcctatg aggcagtaat
      241 ttttttcatc cattacgtag tagtcgtcat ccagatcccg gattggtatc atgccttcgc
      301 acttgttttc agccagttcc acgtagattc cccattcggt aatgcctgaa atcaccccat
      361 cgaaagtttg cccgaggtgc tctgccataa attccacctg tttgtatttt acagatgcac
      421 gctcggcatt ggcggctacc tgctcgcggt tggagcagtg ttcgcagagc tcttcggtat
      481 ctttttcatt cacgcttttg cctccgttca ggtagtgctc gagcaagcgg tgagccatca
      541 tatccggata gcggcggatg ggcgaagtga aatgcgagta gtaatcgaac gccagtccgt
      601 aatgaccaat gttctgggtg gaataaatag cttttgccat ggaccttact gccagtgtct
      661 caatcagatt ttgttctttt ttacccatca catcgtccag cagtttgttt attgccgatg
      721 aaatctgagt cttttttccg gtcgacttga ggttgtagcc aaatttccgg ataaaagctg
      781 agaaattttc aagtttttca ggatcgggag tgtcgtgaat ccgatagaca aaagttttgg
      841 ctttctgtcc tttgcccggt ttaccgatgt gtgttgctac tgttttgttt gccagaagca
      901 taaattcctc aattagtttg ttggaatctt tctgaacctt gaaatatacg ctgagcggtt
      961 ttcctttttc atcaatttcg aatcgaactt cttcacggtc aaaagcaatg gctccgctcc
     1021 tgaaacgttt ttcacgcaat atctttgcca gttcattcaa tttcagcatt tcatctttgt
     1081 aatcacccgt gcctgtttcg atgatgtttt gagcttcctc gtacgtaaaa cgcctgttac
     1141 ttcggattac agttttcact atctggtatg atttaacctc ggcgtactca ttcagttgaa
     1201 aaacgacaga atatgttagt ttttcttcat caggtctgag cgaacagatg ccgttggaga
     1261 ggtgctcggg cagcattggc actgtgcggt caaccagata taccgaagtg gcacgttcgc
     1321 gtccttcttt gtctatgatt gttccgggcc gaacgtaatg tgtcacatcg gctatatgca
     1381 cgcccacttc ccaaagcccg ttttccagtt tccggattga aagtgcatcg tcaaagtctt
     1441 ttgcatcggc cggatcgatg gtgaaagtgg tgacgtcgcg catatccagt cgtttcgaaa
     1501 tttcctgtgg cgtaatgcca gagtctattt ttgcagcttc cgcttctaca ttttccggat
     1561 atttgtaagg caaaccatat tcagccagta tagcatgcat ttcagcatcg ttatcgcctt
     1621 tatcaccaaa aatatcaatg acctcgccca cggggctttt catcgtgtgt ttccattcaa
     1681 tgattttcac gagggctttt tgtccggttt ttccgtcttt cagcttgttt tttggaatga
     1741 gtatatcgct gttgagcact ttgctttgta cgttgaggaa tgcgaagtta tccgatactt
     1801 caaggattcc cacaaaggtg tcctgtgagc gctgaaggat ctcaaccact tcaccttcag
     1861 gttctttacc tttccggtgg gcgtacatga ataccttcac cttatcgttg agcatggctg
     1921 tgcctgtttt acgttcgggg atgaaaacag gttcgccgcc gtcgtccgga atgaggaatg
     1981 atttcactcc ctgacggtca aagcggccga taatgtaacc tctgcgtgaa tttagcttga
     2041 attttccgcg tgaaatctca gtcagcaggt cttcatcgcg aagttggtaa agcagttcgt
     2101 taataaaagc gcgctgtgaa tcggatgtaa tctccatctg gttggagatt tgtttgtaat
     2161 tgtatgtttt ttgcggattg tcggtgaaaa tggcggtgat atttctcaac aggtcatttt
     2221 ttcgtaaacg ggtgcttccc tgttctttcc ttctttttct catggagtga attttttaaa
     2281 atattttcaa atatacaaca aaccacttgc attcaatgtt tctactttga gaaatttaat
     2341 tttatttttc tgaaaattaa tactacattc gcattcataa ccgatacgtt gatgaaaaaa
     2401 aatatagctt ttattgtaaa tccggtttcc ggcacaactt ccaggtggaa tgtaactgaa
     2461 cttattcggg ataatctgga tttttctcat ttcaacgagt gccgaattat gaataccgag
     2521 cgtaaagggc atggcagaga gctttccagg caatttgcag atgaaggttt tgatgttgtt
     2581 gtggcagttg gcggtgatgg tactgtgaat gaagtggcgg gtgcattaat caataccgat
     2641 acggcgctgg gagttatttc cacaggttca gggaatggat tggcacgaca cttgaaaatt
     2701 ccgctgaaca ttaagcaagc cattgagcat ctgaactttg cggaagctat aaaaatggat
     2761 tacgggctgg taaacgacga aaaaacattt ttctgcactt gtggatttgg atttgatgct
     2821 tacgtgagcg aacttttctc caaaggaacg aaacgtggct ggatgggcta cattgagaaa
     2881 atggtaaccg gatattttaa ttatgagcct cagccttacc ggttagtggg cgaagatatt
     2941 gatttgaagg gaaatgcttt tgtgataacg tttgccaatg cttcacaatg gggttacaac
     3001 gcttatattg ctccgaatgc cagtattcag gatggaatga ttgatatttc agtgattacg
     3061 aatgtgccga ttatcgctat tccaaccatt gcatttcagc tttttaccaa aacaatccaa
     3121 aaggaccttt tagttactac actccgaaca gaagaaatta cgctatacag aaataaaccg
     3181 gggctttttc acctggacgg tgatccatac gaacttggtg ataaaattca tattaaaatg
     3241 atgcaggaag gattgaaagt aatggtgaaa aagagatttt aattggagtt tacttaatta
     3301 ttttttcggt aattgttcta cagataatag tcttcctgtt tcggggctta cgtatgcttt
     3361 gctgtattta tccatcgttt caaacggaat tggaaccaaa atacccaatt gcggcaaagt
     3421 cagtttattt tcgtacaata ttttgtttcc atcatcaaat ttcacatcag cagttactgg
     3481 aattactgta aatacttcaa tatctttctt tttagcaggt ttttcaggag tttcgatttt
     3541 aatcggattg tatttaatat tcagataata aggcactccg ctcaaatcat cagcgtcaac
     3601 aacaccttgc attgacgaaa accggaatac aattttatca tcaactgctg aatcaggagt
     3661 gtatacaacg gtttgtgtca gtgtttgtac agtagttttt ccagtaaaaa gttcggtgag
     3721 ttctttttcc tgcgcctcca tttcggcaat catggctttg agcgaagcac cgtcagtagg
     3781 catgttatca acatcgcccg agagcagatc catccggttt tcgcgaatcc ggtaaatgtg
     3841 ttttgctgct ccttcggcca tttttgctac agagccggcc agcatgtatt cttcgctcag
     3901 cggaagcaat tttgcacttt caagatcttc tttttgctca attctttcca ctcgtatctc
     3961 ttttacctgt ttggcgggag caacatttat gccgcataaa attccttcgt cgtttactgt
     4021 tagctggttt gcgagtgatt ttttggatgg aataatggtg aaagttcttt cagcatcgga
     4081 tactgtgcgg gtgtccatcg aaattttttt cagccggtag tattttttct ctgccgtaat
     4141 tacatccgaa gtagccagaa atcgttcgga atactggtaa aattttcccg gaatttccgt
     4201 cactatttcc accgttgcgt tgattacaaa ttctgttttc ggcagcgaat agatgagcgt
     4261 tgactgacct tcggtaatgg tttgtgccat gccagacagg ggaagcagta ttgtgaaaat
     4321 gaaaaaaaat cgtttcataa ttgataaatc ttttttttag gtttatgcat taatcagatt
     4381 ttcagcgagt taaaataaaa tgaatggaaa cacccgatca gagtatgtta ctgggctttt
     4441 tttcttcttc ccgcaaatgg ttttttcttt tttccaaccg tgtcggcctg cgaagtaatg
     4501 atttcgcgac attgttcttc ggtcagagta gcaacatcgg tatttttcgg tattttataa
     4561 ttgttttttt caaaagcaat gtatgcgccg aatcttccgt tcaggacctg tatctcgccg
     4621 ttctgaccaa aaatgtgaat cactttgttt ttgtcgctct cacgtttggc ttcgatgatt
     4681 tcaacggctc tttcggccga aatttccagc ggatcgtcgg ttttcggaag cgagtaaaac
     4741 ttaccgtcgt ggcggatata tggtccgaaa cggcctatag cggcaatcat ttccttattt
     4801 tcaaattcac ctatttcacg tggaagtttg aaaagttcga gtgcttcttc gagagttata
     4861 ctttcgatgg actgatttct gtgcagcgag gcgaaaaccg gtttttcttc ttcttccgac
     4921 ttgccgattt gggctatcgg tccgaatttg ccgattttta cggctatctg tctgccggat
     4981 ttcggatcga tacccaaaat acgttctcca acctgacgtt cagagttttt aagggtgctt
     5041 tccactaccg gatgaaattt tttgtagaaa ttatcaatgg attttgtcca ttttactttt
     5101 ccttcggcta tgtggtcaaa ttctttttct acattggccg tgaaattata atccatgata
     5161 ttcggaaaat attcgaacag aaagtcattg actaccaggc caatatccgt aggaaaaagt
     5221 tttgatttct ctgcgccggt attttcggat tttattttat ctgaaatttt accatcttta
     5281 agttttagca cgttgaaatc tctttttatt gcctgccggt cgcctttctc tacgtaattc
     5341 ctgttttgta tggtcgaaat ggtcggtgcg taagtggacg gacgcccaat gccaagttcc
     5401 tcgagtttgc gtaccagact tgcttcggtg tatctgggcg ggcgctgggt gaatcgttcc
     5461 tgagccagaa tttcattgga agtgagtgat tcaccttttt tcatcgtggg taaaatgccg
     5521 cttgtttccg catccggctc atcatcagtc gattcgagat agactttcag gaaaccgtca
     5581 aaaataatca cttcgcccga agcgatgaat tgatgattgc tgcccgagat attgatgtat
     5641 atgtttgtct tttccagttc ggcatcggcc atttgagaag cgatggtgcg tttccaaatc
     5701 aggtcgtaca ggcgcttttc ctgcgccgtt ccttcgatgg tatgactgct tatgtaggta
     5761 ggtctgatag cttcgtgggc ttcctgagca ccttttgttt tggtagagaa ctgacggatt
     5821 ttcacgtatt tttcaccagc catttgaatg atttcctgct tggcagtgcc gatagccagc
     5881 gccgacagat tgacagagtc ggtacgcatg taggtgattt tacctgattc gtagagtttt
     5941 tgcgctacca ccatggtttg agtaaccgag aaacctaatt tccgcgatgc ttcctgctgc
     6001 agggtagagg tggtgaacgg aggtgccgga gattttttca gtggtttttt ctcgatgctg
     6061 tccaccgtga atttggcact tttgcatagt tcaagaaatg cgagtgcttc ttctttggtt
     6121 ttgaatcgct gtgataattc agctttgagt tcaacattcg taccggcggc atctttagaa
     6181 gtgaaaagtg ctgatactct gtaagatgct tccggattga aattgttgat ttcccgttcg
     6241 cgctccacaa tcagacgaac tgccaccgac tgaacacgcc ccgccgaaag tgacgggcgt
     6301 actttacgcc acagaaccgg tgaaagttcg aaacccacaa tgcggtccag tacgcgccgt
     6361 gcctgctgtg catccaccag attgatgtca atttcgcgcg gattttcaac ggcgtgaagt
     6421 atggcatctt ttgttatttc atgaaaaaca atgcgcctgg tgttttcttt ctttaatttc
     6481 aattcttctg ccagatgcca tgcaatggct tctccctcgc ggtcttcatc ggatgccagc
     6541 caaactgttt cggagctttt tgccgctttt ttcagttccg aaacaatttt tttcttgtcg
     6601 gccgagacct cgtactgcgg cgcgtaatta ttctcaaaat cgattcccaa acctttttcc
     6661 gacaaatcgc ggatatgtcc catactcgac atcacttcga attcgtttcc aagaaacttt
     6721 ccgatggttt ttgctttggc aggtgactca acaatgacta aattttttga cataattacg
     6781 tttatttttc aagttgggtg caaaataaca aaaacaaaca ggtataagcc aaattttcag
     6841 tgaattattt ttgtttttta tgaaaatata aacggataaa tgtttgtaaa ttacgattat
     6901 ttttcagaaa atgcggtaat ttgagttact tcgtatccga catcgtcaac ggcattttta
     6961 attacgtcat cggtgacttc ttttgtgaga ttaatcctgg ctgttctgct tttcaaatct
     7021 actattgctt ctactccatc tattttattc aatgcttttg atactctggc tgcacagtgt
     7081 gagcagctca ttcctctgat aattgctgtt ttttccataa tttgtttttt attggttggt
     7141 acgttttttt cgatttgtaa tattttttat ttttttgatt ttgtcggttt aaagttcttc
     7201 aatcttaagg cattggttac cactgtaata gaactcaggc tcatggctgc tgcagcaaac
     7261 atagggctga gtttccatcc taacgataaa taaaacacac ctgcagccag cggaataccc
     7321 agtatattgt aaaaaaacgc ccaaaacaga ttttctttga tatttcggat tactgattta
     7381 ctcaatcgaa ttccggtaac cacatccacc aggtcgtttt ttataagaac tacatctgcc
     7441 gcttctatgg ctacatctgt gcccgagccg atagcaaatc ccaaatccga cctggcgaga
     7501 gccggagcat cgttaattcc atcgcccacc atggccactt ttttaccttc tgcctgcaga
     7561 ttttgaataa tcttgtcttt gtcctgtggc attacttcgg ctatcacgct gtcgaaacct
     7621 attttatcct gaatggcaaa agcggtacgc tgattatcgc ccgtaagcaa tacggtgtac
     7681 atgttcattg atttgagcat ttcaaccgct tcccggcttg taggtttcaa tacgtcggct
     7741 actccgatta taccgagcac gttttcatca tcggcaacga aaagagctgt tttcccttgc
     7801 tgcaacagct ggaataaaaa atcttcaacc tcttcaatgt caatgctttc tgaaataaat
     7861 gccttgttgc ccactttgta aaattcacca ttaatgattc ccgaaatccc tttaccggga
     7921 atactggtga actcagtcac aggatacaaa tccagctttt catcttcggc ttttcgggta
     7981 atggctgcag aaatgggatg ttcggaatat ttctccagag aggcacaaat tctcaacaat
     8041 tctttttcat ccatataatg aatggactca atgtcggtaa cctctggctt tccctcggtg
     8101 atagttccgg ttttatcaaa tacgatggtg ttgattttat caccggtttc gagtgcttcg
     8161 gccgatttga aaagaatgcc atgctctgcg cctttgccag tgccaaccat aatggccacg
     8221 ggagtagcca gccccagagc acacggacat gaaataatca aaacggatat accgatggat
     8281 aatgcaaatt ctgcagaata acctgaaatc aaccaaaaaa aggtagctaa aaccgcaatg
     8341 actataacta cgggcacaaa tacaccgcta attcgatctg caagtttgga gatgggagct
     8401 ttggaggcag acgcctcttc gagcagatgg atgatttgcg aaagcgtagt gtcattgccc
     8461 acttttgtgg cacgaaaggt gagaaatccg tttttattga tgcttgcaga aatcacctta
     8521 tcacctgtct gtttgaaaac gggtatgctt tcgcccgtaa gtgccgattc gtcaacagaa
     8581 ctgaaaccgc tttccacaat gccatcaacc ggaattttct cgcccggacg gatgcttatc
     8641 aggtcgccgg ctacaacttc ctcaaccgga atttcaatct cctttttatc gcgaatgacg
     8701 ctggcagtct gaggagctga atttatcagt tttgtaagtg catccgaagt ctggcgtttt
     8761 gaacgggctt cgaagtattt tccgagagta atcagcgtaa gaatggtagc acccgattca
     8821 aaaaataaat catgcaaata atgatgaatg atgcccgaat cgttgttgag agaagcatta
     8881 aaaatccgga aaaccgcaaa aatcccgtag agcaatgctg ctgaagaacc tattgctacg
     8941 agtgtatcca tggtaggagc cagtttccac aacgaacgaa agccattgga gaaaaattta
     9001 cggttgatta tatacacagg tacagtaagc agaagttgag taagtgcaaa actgagagcg
     9061 cttttttcgc ctgcaagaaa tgcaggtaat ggaaaatgga gcatgttgcc catggatata
     9121 taaagcaagg gtataagaaa gataaatgac agtacaactc tttttttcat tgcaagttgt
     9181 tcattctctg catgattaac cttcaaatcg ttgtctgaag gagtctcggc cttcaaataa
     9241 gcatcgtacc ctgcatcctg aaccgattca ataatattga gagcagaaag tgagttttga
     9301 tcgtaattta cagacatgct gttggttagc agattcacag ttacgtcttc cactccattc
     9361 agcttccgaa cacttttttc aacatgagcc acacatgccg aacaggtcat gccggttaca
     9421 tcaaattttt tcgtttccat attgcttatt gataatccga aaaacagatg aaattcatat
     9481 ttggttcaaa aataagtggt tatttacatg aatttaagga tggttaactg taatggtaaa
     9541 ggagacggag agaaaatgga ggacgatgtg atttaaaaac aagaaaaggt agccacgcct
     9601 gactaccaat cctttaacct taaatctaat actatgaaaa aaatcacgat gcaaatatat
     9661 aaatcaattt gagttttcac aataaactga ctaaatattc tttgagaatt aatgttattt
     9721 aactattgat gttttgaacc taattcgctc atttatttat atttacaggg ctattaataa
     9781 atatatttaa tatacaaacc gtagttattt gtttttattg ttaataaaat gcaattttaa
     9841 aatcgggctt cagtgcatga aacttgctca tgtaatgatg agatcgaatg tttacacgag
     9901 tatagttgct gctggtgata ctgtgtggta ttacagaaca ttaaatttaa aaaatatgaa
     9961 gatatttagt aactatattt taattgatta attagtttct gaatagagct taatctgatg
    10021 gatcttgtaa gtaattgatt tatccataat aattgacaaa cagatttcaa aacaggtaga
    10081 aatgttttga caacggaatg ttttataaaa aaatccgaaa aatttaaatc caaacaaagt
    10141 attcgttacg tctaattcat aaacaaagat tatatttaca aaccaattaa aattttacag
    10201 aaaatgaaac gttttggatt tttattagta ggaataatcc tgtggattgg agcaagtgca
    10261 caggttcaag cagataccac tcaggttaca attcccgaca gtgttatcga aaagtacgca
    10321 gctaccagtg agaatacacg tgaagccatt ctattgcaga agcttaatcc cgatcagtta
    10381 ctcgaaatag aaaaacaacg actggacaac gaaagacgca gggagagcaa tgaaatacct
    10441 atgccaatgt gggcactggt gctcatatcc actgcacctt ttgtaatggt ggttttcatt
    10501 gttttcttta ccagcagagc caaaaaggaa agggaaaaag cccgttacga tctttatctg
    10561 aaatccatcg aaatgggtca gccgctaccc gagaagattt ttgaagaacc ggaaaaaaag
    10621 gtatcaagac tgcaaaacgg actggtatgg cttggaatag gtttagcgct ggtcattgta
    10681 ggcattacaa cccgcaatga tggactgtta ttcggaatgg tacccggttt tatcggattg
    10741 ggaattttga ttgcatatct gattgaaaaa ccaaaagata attcaggatc atcggccata
    10801 aatgaataat aaggcagaag atttgctttg gatagcgcag gtggtcgtcc gtgacgaccg
    10861 ccgtgcgttt gaccgcctgg tatgcaagta tcagtcgccc ttgagaaggt ttttgctgaa
    10921 cctaaccctg ggcgactcag ctttatctga tgatttggca caggaaacgt ttatcaaagc
    10981 ataccttaat atccgctcat tccagggcgt ttcagcattt tccacctggc tttaccgtat
    11041 tgcttacaat atatactatg attcagtaag gtcaaacaaa agatacgaag atctgaacga
    11101 acctgaaatt gataaaaaaa catccgtaag tattgaattt tcggcagaaa aatccgatat
    11161 ttacaccgct ttacaacttc tcagaaaaga agaaaaagca gctatcctgc tttgctacat
    11221 ggaagaaatg acccataaag aaattgcagg cattatgaaa ttaccattgg ggactgtcaa
    11281 aacccacata ttgaaaggta aggaaaaaat aagtaacttt ttaatacatc agggatatgg
    11341 aaaataagga agatgtgctg atgagagatt ttttcggaaa aaacagggaa gagattcctg
    11401 ataatggttt ttcagattta gtaatacgcc ggttgcccgg gaaaaaaaga aaaacaaact
    11461 ggattgtgcc tgtatttgcg ttgataggca ctgttatttc attgtttgtg attgatattc
    11521 acgaagttgt actcagagca gttgaattgg tagccggtat tccctttgtt tatttactgg
    11581 ccggatttat gatttttcca atcacaatcc tgtttttgta ttatttctgt gaaaaggaaa
    11641 gagccttttg actcagagaa gggctaaaac acttaaaacg ataacaaata aaatgatgaa
    11701 acagccatta aaagctactc ttgacacaca acttgtcccc aggtatttcg ggaagcaaat
    11761 gtcccgatag ctatcgggag gcatgttccc tacctgtagc agtcaggcat gtgtccttaa
    11821 taaaaaaaaa taaacacatg aaaaggtcct gctttcttct cttgttattc gcaggtgttc
    11881 tctcttgtaa tcatcatgcc ggaaaaaaca acagcgaaac aatcccggaa actgacagcg
    11941 ttgttcaact tgcaccgatt gacacattgg cgctcacgct ggatgcctta cgaaacaagc
    12001 ctctgcaaac tggttacaaa atgattggag atgttagaac tgctattctc acttatgcag
    12061 atttcagaat gggagatgct gcactatata ttttcaaaaa cgaaaccgga gatgaaattg
    12121 cttttagcgg caatgatact aaggtcccgc ttacagtaaa atccatgcaa ccaaccattg
    12181 aaaacggagg aaaaaatcca aatcccttgt ttttgaataa gcgattccgc gtagtttggc
    12241 gcaggataca gctgaatcac aaacctcaaa acgaaatgga actttactac caagagtacg
    12301 atgaaatcat atatctgaaa gaaatgtagc agatgtaact tattatacac ctgctcattg
    12361 ttc
//
LOCUS       DAORKP010000059        12315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_503_length_12315_cov_9.506525, whole genome shotgun sequence.
ACCESSION   DAORKP010000059 DAORKP010000000
VERSION     DAORKP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12315
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_12315_cov_9.506525"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLN75_04770"
     CDS             complement(<1..487)
                     /locus_tag="PLN75_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03463.1"
                     /translation="MKYIISGGGTGGHIFPAISIANAIKAKDPKADILFVGATGRMEM
                     ERVPAAGYPIEGLPVSGFDRKNLLKNFRVLIDLQKSIVKARKIIRNFSPDIAIGVGGY
                     ASAPTLKAASRLGVPTLLQEQNSYAGVTNRMLASKASAICVAYEGMEKFFPKEKIILT
                     GN"
     gene            complement(577..1815)
                     /locus_tag="PLN75_04775"
     CDS             complement(577..1815)
                     /locus_tag="PLN75_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRG03464.1"
                     /translation="MGNKFSKLLKGDTTIWIVFITLCLFSVIELYSASSTLAFKAANH
                     TAPMLQHVTFLAFGALLAYFVHLIPYKYIRMLAYPGLILSFFLLIYVLLRGESANEAN
                     RWIRVFGIQFQPSEVAKLSLVIVIADFVSRIRANPSQEKKYFIWILGVAGLIIGLILL
                     ENFSTAAILGLVVFSMMFMSQLSWKKLAGILGFIVVVLLVGYILVKTIPKEKMPGAFD
                     RAYTWVARIERSANETDESKYEINDENLQVQHGRIAVARGGFFGVFPGNSVERDYLPH
                     AYDDFIYAIVVEEMGLAGGIFVMFLYLVLLFRTGQIATVCKSTFPAMLVTGLGLLITL
                     QATVNMFVGSGFGLVTGQPLPLISRGGTSILVTCIYFGIILGVIREIKSENEKIYRKK
                     EPEVDDIPQNDEIVMEINEI"
     gene            complement(2014..3348)
                     /gene="murD"
                     /locus_tag="PLN75_04780"
     CDS             complement(2014..3348)
                     /gene="murD"
                     /locus_tag="PLN75_04780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRG03465.1"
                     /translation="MKRIVILGAGESGAGAAILAAKQGFDVFVSDFGEIKHQYKLLLD
                     KHHIRWEEKQHTESLILNADEVIKSPGIPDKAPMVKALREKKIPVISEIEFAGRYTKA
                     KMICITGSNGKTTTTMLIYHILKEAGLNVGLAGNVGDSLALQVAEKNFDYYVVELSSF
                     QLDGMTEFKADIAILMNITPDHLDRYNYEFQNYIDSKFRIIQNQTEKDAFIFWNDDPV
                     IRNELKKRNIQSAIYPFSLEKEEKTKAFIENNQLIINTLNNIFTMPASELSLTGIHNT
                     YNSMAAGLAASIISVRKENIRQSLQDFQGVEHRLEYVATVRDVKYINDSKATNVNSCW
                     YALQSMRTPVVLILGGTDKGNDYSEIEELVKEKVTGMIFLGVDNEKLHLFFDGKVEKI
                     ADARSMQECVQAAYNMAKPGETVLLSPCCASFDLFENYEDRGNQFKVCVRNL"
     gene            complement(3351..4616)
                     /gene="mraY"
                     /locus_tag="PLN75_04785"
     CDS             complement(3351..4616)
                     /gene="mraY"
                     /locus_tag="PLN75_04785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRG03466.1"
                     /translation="MLYHLIQYLEKSYDIPGQQMFVSISFRTGVAFILALLVATVFGR
                     KIINRLQKMQIGETIRDLGMEDQLKKKGTPTMGGIIIILAILVPTLLLADLTNIYSLL
                     MIVTTVWMGIIGFADDFLKIKYKNKEGLKGKFKIFGQVGLGLIVGLTLYASPDAVIRE
                     NVEHIGISQRVEDVSYKAEGVKSTKTTIPFFKNNNLDYANAFKNAGENAQKYGWILFV
                     IMAIFVVTAVSNSANLTDGMDGLATGSTAIIGVTLGILAYLSGNINYAGYLNIMYIPG
                     TGEMVVFAGALIGATIGFLWYNSFPAQVFMGDTGSLTLGGIIAVFSIAIHKELLIPIL
                     CGLFFVESLSVIMQRLYFKYTKKKFGEGRRIFLMSPLHHHFQKGGKSGIEAIIKKPFN
                     PVHESKLVVRFWLIGIILAAITIITLKIR"
     gene            complement(4727..6178)
                     /locus_tag="PLN75_04790"
     CDS             complement(4727..6178)
                     /locus_tag="PLN75_04790"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRG03467.1"
                     /translation="MKLKEIIQNIKVENITGNPETVITGIQFDSRKIESGNLFVATRG
                     TASDGHTFIPAAIENGAAAIICEDIPETTDNSITYIQVKDSADALGHAASAWYGFPSS
                     KLILTGITGTNGKTTTATLLYKLFRTLGYGAGLLSTVQNFINDDAIEATHTTPDALAL
                     NELLAKMVESGCKYAFMEVSSHAVEQRRIAGLKFVGAVFTNITRDHIDYHLTFDNYLK
                     AKKRFFDELDSNAFTLTNIDDRNGLVMLQNTKAARHTYSCRGIADFKTRILEDGFDGM
                     MLDMNGKEVHVSFIGNFNAYNLTAVFGTAVLLGQDELEVLRIISTLKSVSGRFETIQA
                     PAGFTAIVDYAHTPDALENVISTINEILQGKGKLITVVGCGGNRDKGKRPMMAREAVN
                     GSWKAILTSDNPRNEEPMDIINDMIAGLDNEQKAKSLTIVDRREAIKTACAIAQKGDV
                     VLVAGKGHEDYQIIKGVKYHFDDKEEVRKAVFL"
     gene            complement(6186..8327)
                     /locus_tag="PLN75_04795"
     CDS             complement(6186..8327)
                     /locus_tag="PLN75_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRG03468.1"
                     /translation="MTEPGKKIIGRFATVYIIIVILMMSIIYNIAKIQTVERENWLKL
                     GEKNNKNDIIVRPNRGNIYSADGRLMASSIPTYYVYMDTRVPALHEDDGKLFAENIDS
                     LSICLSDYFGDKSSEAYKRDMTAAYRQGKGEYNFYKGKISYAQLKDIRRFPLFRLGRN
                     KSGLLTKEMFSRVKPFTSLASRTIGDIYADESRGGKNGLELGFDSILKGTPGLSSRQK
                     VANRWEEITQVEPVDGMDIVSTIDVDMQDITEKALLDKLKEIEAQTGYAILMEVATGE
                     IKAIVNMDRNPDGSYTENRNGAVSDKVEPGSTFKTVSLMAVLDDGKAKLTDVIETGNG
                     LYKFGKATMRDHNAHKGGYGSITLEQSLNASSNVGISRTIYRAYGDNPGKYVDKLYKM
                     GLNEPFQLYIPGSAKPWIRHPNDKSSYWSATTLPWMSIGYEVQIPPIYTLAFYNAIAN
                     NGKYIEPLFVKSINKNGLMVREFKARVINSQICKPSTLKEIRQALLGVVENPKYGTGK
                     AVHSPYVRIAGKTGTAQISKGSAGYSAGGKSHQVSFCGFFPYENPKYTCIVVIREPKI
                     GYPSGGLMSGVVVKNIAERISAIDTVSTIFDLQLDSTYTEIKKPLVKSGKYEAIQNVM
                     TNLQQSYGGEGASWVKTISDGKTTNLTNLPLVAGTVPDMTGMCAKDAIYLCEKLSLKI
                     NMKGIGKLTSQSLSPGTKTAKGQEITLIFEN"
     gene            complement(8333..8800)
                     /locus_tag="PLN75_04800"
     CDS             complement(8333..8800)
                     /locus_tag="PLN75_04800"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRG03469.1"
                     /translation="MSFWNKIVEVVKGNEDFSDLKSSTFRDVLNGNILSKNFIKKQYG
                     LFLLIAVLTFFYVDNRYYCEKQVSKEVKLKKELQDVKFESLTISAELTKLSRRTYVLD
                     YIEAKGLNLKESPYAPVVIEEPDPVKDEEVKKQKEAHKAATVKVKSDSTHIEE"
     gene            complement(8849..9742)
                     /gene="rsmH"
                     /locus_tag="PLN75_04805"
     CDS             complement(8849..9742)
                     /gene="rsmH"
                     /locus_tag="PLN75_04805"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRG03470.1"
                     /translation="MPALLAETIEGLNIKPDGVYVDVTFGGSGHSREILKHLGAKGRL
                     VGFDQDEDALQNMIDDERFTFVRSNFRYLTNFLKYHGVEKVDGILADLGVSFHHFDEA
                     ERGFSFRFEGKLDMRMNTRSSFTAARVVNEYSEEQLADVFYLYGEMHNSRRIASAIAK
                     ARSMGRIESIEQFLEVLKPFFPREREKKEMAKVFQALRIEVNKEMKVLQELLSQSVET
                     LNENGRLVVLTYHSLEDRLVKNFIRSGNFEGKQVKDFYGNIIAPLKAVNNKVITASEE
                     ETARNPRARSAKLRIAEKLKN"
     gene            complement(9860..10315)
                     /locus_tag="PLN75_04810"
     CDS             complement(9860..10315)
                     /locus_tag="PLN75_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRG03471.1"
                     /translation="MSTFIGKYDAKADVKGRIFIPSVYRKVLRDGEKDRLVMRKDADN
                     DCLVLYPESVWNKKIEELKLNLDEWDAGDQLLLMQFVSDAEWLDIDSQGRVLISKKYL
                     DLIGIENNEVMFMGMMDRFAVWGRQRYEEVKLPAGDFAKRLKDKMKTAP"
     gene            10664..11500
                     /locus_tag="PLN75_04815"
     CDS             10664..11500
                     /locus_tag="PLN75_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRG03472.1"
                     /translation="MEEKVMRFDVEEIIRAKAPEKKFPKFIVNYLKKILHQDELNEFF
                     VRAHGKKNVEFIDFALGEGLLRVTADFEGVENLPTDGNRYIFVSNHPLGGLDSVILGL
                     LLGRKYEGKVKYFANDILMFLEPMKDMFLPVSKVGKASDVRENAKAVEAFFNSDNQLI
                     TFPAGTCSRKVHGKIQDLPWKKSFVSKAVQSKRDVVPIFFNGRNSNFFYNLSIIRNKL
                     GLPNIEMLYLVNEMFKQHGRHFKIKIGKPIPYATFDKSKTPVEWAEWVRSKVYELEKQ
                     IK"
     gene            complement(11521..12018)
                     /locus_tag="PLN75_04820"
     CDS             complement(11521..12018)
                     /locus_tag="PLN75_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HRG03473.1"
                     /translation="MIKISNTNLQQAGAIGVNEFLQVFTDAYLEALGGNLNAENMHLL
                     NGHQHTLLAFHFLREEVMYGGFVQLIQNGYGSYIFDNPTAKALKQMGASDLAKIIYKA
                     REIYDANRAELERETTEEEFTAMYVDFEQFDELEERFFEIEEEQTAIIARYVDDNIAE
                     FGEIK"
BASE COUNT         3068 a         2741 c         2592 g         3914 t
ORIGIN      
        1 gatttccggt aagaatgatt ttttctttcg gaaagaattt ctccataccc tcgtaggcca
       61 cacagattgc cgatgctttg gaagcaagca ttctgttggt cactccggcg taggaattct
      121 gttcctgaag cagggtgggc actccaagtc gtgaggctgc tttcagcgta ggtgcgctgg
      181 catacccacc cactccgatg gctatatcgg gactgaaatt gcggataatt tttcgggctt
      241 tcacaatgct tttttgcaaa tcaatgagca cgcggaagtt tttcagcagg ttttttctgt
      301 cgaatccgct taccggaagc ccttcgatgg gatagcctgc agccggaaca cgttccatct
      361 ccattctgcc tgtagcaccc acgaacagaa tatctgcctt cggatctttg gctttaatgg
      421 cgttggctat gcttattgcc ggaaaaatgt gtccgccggt acctcctccg ctgataatgt
      481 atttcataat tgctgctaag ttaagttttt tacctttttt ataaaaataa gatgtcaggg
      541 gtgatttttt tacctgtaat ttcctttttt tcaggattat atttcattta tttccatcac
      601 tatttcgtcg ttttggggaa tatcgtccac ttcgggttct tttttgcggt aaattttttc
      661 attttcggat tttatttcgc gtattacgcc cagaatgatt ccaaaataga tgcacgtaac
      721 cagaatggaa gtgccaccgc ggcttatcaa gggcaaaggc tgccccgtaa ccagtccaaa
      781 tccggatcct acaaacatgt ttaccgtagc ctgcagggtt atcagcagcc ccagtcccgt
      841 aaccaacatg gccgggaagg tgcttttaca cacggtggct atttgtcctg tcctgaaaag
      901 aagtacgagg taaagaaaca tgacaaagat accacctgca agtcccattt cctccactac
      961 gatggcgtaa ataaagtcgt cgtaggcatg aggcagataa tcgcgctcaa cgctgttgcc
     1021 ggggaatact ccgaaaaatc cgccccgcgc cacggctatt cttccgtgct gcacctgcag
     1081 attttcatcg ttgatttcgt acttactctc gtcggtttca tttgccgacc gttcgatgcg
     1141 ggcaacccac gtgtaggcac ggtcaaatgc tcccggcatt ttttctttcg gaatcgtttt
     1201 taccagtata tatcctacca gcaatactac tacgatgaag cccaggatgc cggccagttt
     1261 tttccacgat agctgactca tgaacatcat agaaaatacg actaaaccca aaatggctgc
     1321 cgtcgaaaaa ttttcgagca gtatcaggcc aattatcaac cccgcaacgc ccagaatcca
     1381 gatgaaatat tttttctcct gtgacggatt ggctctgata cgcgatacaa aatcggcaat
     1441 gacgataacc agcgatagtt ttgcaacttc cgaaggctga aactgaatgc caaaaacacg
     1501 tatccagcgg ttggcttcat tggcgctttc gccacgcaaa agtacgtaaa taagcagaaa
     1561 gaatgataag ataagccccg gatatgcaag cattctgata tatttgtaag gtatcagatg
     1621 gacaaaataa gccagcaagg caccaaatgc cagaaaagtg acatgctgga gcatgggagc
     1681 ggtatggttg gcagccttga aagccagtgt actcgatgca ctgtaaagtt caatgaccga
     1741 aaacagacac aaggtgataa acacgatcca gatggtggtg tcacccttca ggagttttga
     1801 aaacttattt cccatacttt ttttagttag aaaccaattg acagataaca gataacagaa
     1861 gtcagaattc agatgaaaga ttttagatct cagataattg gtttctattt taaaatgatt
     1921 ttcacattaa ctacctgaag gatacactta tcaaccaatc tcccgatagc tatcgggaca
     1981 attaaccaat taaccaatcc accaatctcc cgatcaaaga tttcttacgc agactttgaa
     2041 ctgatttcca cggtcttcgt agttttcaaa cagatcaaaa ctggcacagc agggcgaaag
     2101 caaaactgtt tcgccgggtt ttgccatatt ataagctgcc tgaacacatt cttgcatgga
     2161 acgtgcatcg gctatttttt caacttttcc gtcgaaaaaa agatgcagtt tttcgttgtc
     2221 aacaccaaga aaaatcattc cggtaacttt ttcttttacc agttcttcta tttcggagta
     2281 atcattcccc ttatcggtgc cgcccagaat tagcacaacc ggtgtacgca tgctttggag
     2341 tgcgtaccag caggagttta cgttggtggc tttggagtcg ttgatgtatt ttacatctct
     2401 gactgtggca acatactcca ggcggtgctc aacaccctga aaatcctgca gcgactgccg
     2461 gatgttttcc ttgcgtacgc tgataataga tgctgccaac ccggctgcca tggagttgta
     2521 ggtattgtgt attccggtaa gtgatagttc tgatgcaggc atggtaaata tgttgtttaa
     2581 tgtatttata atcagttggt tattttctat aaatgctttt gttttttctt ctttctccag
     2641 tgaaaagggg tagatggccg attgaatatt ccgtttcttc agttcgttgc gtatgaccgg
     2701 atcgtcgttc cagaagataa atgcatcctt ttcggtctga ttctgaatga ttctgaattt
     2761 cgaatcgata tagttttgaa attcatagtt gtatctgtcc aggtgatcgg gtgtaatatt
     2821 catcagaata gcgatatctg ccttaaactc cgtcatccca tcgagctgaa aactgctcag
     2881 ttccactaca taatagtcga aatttttctc ggccacctgc agtgccagac tgtctcccac
     2941 gttgccggcg agccccacgt tgagtcctgc ttctttcagt atgtggtaaa taagcatcgt
     3001 agtggtggtc tttccgttgc ttccggtgat gcaaatcatt tttgccttcg tgtagcgacc
     3061 tgcaaattcg atttctgaaa ttaccggtat ttttttctct ctcagcgctt ttaccatcgg
     3121 agctttgtca ggaattccgg ggcttttgat tacttcatcc gcattcagaa tcaacgattc
     3181 agtgtgctgc ttttcttccc accggatgtg gtgcttgtcc agcagtagct tgtattgatg
     3241 ctttatttct ccaaagtctg aaacaaaaac atcaaatccc tgcttcgctg ccagaatggc
     3301 tgctccggca ccgctttcac ccgctccaag tatgacaatt cgtttcataa ttatctgatt
     3361 ttaagcgtta tgatggttat tgcagccaga ataataccta tcagccagaa tcgcacaacg
     3421 agtttcgact cgtgaacggg attaaatggt tttttaatta tagcctcgat tcctgacttt
     3481 ccgcctttct gaaaatggtg atgcaggggc gacataagaa aaatacgtcg tccttcgccg
     3541 aattttttct tcgtatattt aaagtaaagc cgttgcataa taaccgataa actttcgaca
     3601 aaaaacaatc cgcataaaat cggaatgagc agttctttgt ggatggcaat agaaaataca
     3661 gcaatgattc caccaagtgt aagacttccg gtatcgccca tgaatacctg tgccggaaag
     3721 gaattatacc agagaaatcc gatagttgct ccgatcagtg ctccggcaaa aaccaccatt
     3781 tcaccggtgc ccggaatata catgatattc aggtagccgg cgtagtttat attgcccgac
     3841 aagtatgcga gtatacccag cgtaactccg attattgccg tagatccggt agccagtcca
     3901 tccataccgt cggtaaggtt ggcactgttc gaaacagccg tcactacaaa tatggccatg
     3961 attacaaata atatccagcc atatttttgc gcattttcac ctgcattttt gaatgcgttg
     4021 gcgtaatcca ggttattatt tttgaaaaaa gggatggtgg ttttggtgga ttttacaccc
     4081 tctgccttgt agcttacatc ttctacgcgt tgtgaaattc cgatgtgttc cacattctca
     4141 cgtattaccg catcagggct tgcatacagc gtcagtccga cgattaaccc caaaccaacc
     4201 tgcccgaaaa tcttgaattt gcctttcaat ccctctttgt tcttgtattt aattttcaga
     4261 aagtcgtcag caaatccgat gattcccatc catacggtgg taacaatcat gagaagacta
     4321 taaatgttgg taagatcggc cagcagcaat gtagggacaa gtatggccag aatgatgata
     4381 attccgccca tcgtaggtgt ccctttcttt ttcagctggt cttccatgcc gagatcgcgg
     4441 atggtttcgc ctatctgcat tttttgcaga cggttgatga ttttacgccc gaaaacagtg
     4501 gccacaagca acgcaagtat aaacgccaca cccgtacgga atgaaataga tacaaacatc
     4561 tgctgacccg gaatgtcgta agatttttct aaatactgga ttaaatggta tagcataata
     4621 gccccctcaa tcccccaaag gggaaattta agattttaat attgttaaaa aattgtattt
     4681 catctgttta tagcacgaaa aaagtatttt tcctttcgaa agcgattcag agaaagaccg
     4741 cctttcttac ctcttcctta tcgtcaaagt gatatttcac tccctttatt atctgataat
     4801 cttcatgtcc tttaccggca acgagtacca catcgccttt ttgagcgatg gcgcaagctg
     4861 ttttgatggc ttcgcgccgg tccacaatgg tcagactttt tgctttctgt tcattatcca
     4921 gaccggcaat catgtcgttg ataatgtcca tcggttcttc gtttcgcgga ttgtctgatg
     4981 tcagaatggc tttccagctg ccgtttacgg cctcacgagc catcatgggt cgttttcctt
     5041 tgtcgcggtt gcctccacat cctaccacgg taatgagttt tcctttaccc tgcagaattt
     5101 cgttgatggt cgaaataacg ttttccaatg catcgggtgt atgtgcgtag tcaactattg
     5161 ctgtaaatcc tgccggtgcc tgaatggtct caaatcttcc tgaaacggat ttcaatgtgc
     5221 ttataatgcg tagtacctcc agttcatcct gcccgagcag tactgctgtg ccaaaaactg
     5281 ccgtgaggtt gtaggcattg aaattgccga tgaatgatac gtgcacctct tttccgttca
     5341 tatccagcat cataccgtcg aatccatctt ccagaattct ggtcttgaaa tcggcaatac
     5401 cacgacagga gtaggtgtgt ctggcagctt ttgtgttttg cagcatcaca agtccgttcc
     5461 tgtcgtcgat gttggtgaga gtaaaagcat tgctgtccag ctcgtcaaaa aatctttttt
     5521 tagctttcag gtagttgtcg aacgtgagat ggtaatcgat atgatctcgt gtgatgttgg
     5581 taaaaacagc tccgacgaat ttcagtccgg cgatgcgtct ttgttccacc gcatgtgagc
     5641 tgacttccat gaatgcatac ttgcaaccgc tttccaccat ttttgccagc agttcgttga
     5701 gtgccagtgc atcgggtgtg gtgtgtgtgg cttctatggc gtcgtcgttg ataaaattct
     5761 gaacagtcga caataaacct gcaccatatc cgagtgttct gaaaagtttg tacagcaatg
     5821 ttgctgtggt tgttttgcca tttgtgccgg taatcccagt cagtatcagt tttgacgaag
     5881 gaaaaccgta ccacgctgat gctgcatgcc ccagtgcatc ggcactgtct ttcacctgaa
     5941 tgtatgttat tgaattgtct gttgtttccg gaatgtcttc gcaaataatt gctgcagcac
     6001 cattttcaat ggccgcagga atgaatgtat ggccatcgga agcagtacct cttgtcgcaa
     6061 caaacaggtt gccgctctct atctttcgcg agtcaaactg gattcccgta atcacggttt
     6121 caggattacc ggttatgttt tcaactttaa tattctgaat tatttctttt agtttcatgt
     6181 gtttcttaat tttcaaatat taatgtaatt tcctgtcctt tggctgtttt ggttccgggc
     6241 gacagacttt gtgatgtcag tttgccgatg cctttcatat ttattttcag actcagtttt
     6301 tcgcacagat atatagcgtc tttggcacac atgcctgtca tatccggaac cgttccggct
     6361 accagcggca ggttggtgag attcgtcgtt tttccgtcac tgatggtttt tacccaactt
     6421 gccccttcac ctccgtaaga ttgctgcagg tttgtcatta cgttttgaat ggcttcatat
     6481 tttcccgatt ttacaagcgg tttttttatt tctgtataag tgctgtcgag ttgcaggtca
     6541 aaaatggttg acacggtgtc aatggctgaa attctttcag caatgttttt caccacaact
     6601 ccggacatga gtccgccgga agggtatccg atttttggct cacggattac tacgatacag
     6661 gtgtatttgg gattttcata agggaaaaaa ccgcagaatg acacctgatg tgattttcca
     6721 cccgccgaat agccggcgct tccttttgaa atctgtgctg taccggtttt accggcaatg
     6781 cgcacatagg gtgaatggac tgcttttccg gtgccgtatt tgggattctc gaccactccc
     6841 agcagcgctt gccggatctc tttcagtgtg gaaggtttgc atatctggct gttgattacc
     6901 cttgctttga attctctcac cattaatccg tttttattaa tggatttgac gaaaagtggt
     6961 tcgatgtatt tgccgttgtt ggcaatggca ttgtaaaagg ccaacgtgta tattggcggt
     7021 atctgaacct cgtaacctat agacatccaa gggagtgtag tagctgacca atacgatgat
     7081 ttgtcgttag gatggcgaat ccacggtttg gccgagccgg gaatgtaaag ctgaaatggt
     7141 tcgtttagcc ccattttgta aagtttgtca acgtattttc ccggattgtc gccataagcc
     7201 cggtagatgg ttcttgaaat gcccacgttg gaagatgcat tcagcgattg ctcaagtgtg
     7261 atggaaccat aaccaccctt gtgggcgttg tggtcgcgca tggttgcttt tccgaatttg
     7321 tatagcccgt tacctgtttc aattacatcc gtgagtttgg cttttccgtc gtcgagcaca
     7381 gccattaacg aaactgtttt gaacgtggaa cccggttcta ccttgtcgga tacagctccg
     7441 ttacggtttt cggtgtagct tccatcggga tttctgtcca tatttactat ggcttttatt
     7501 tcgccggtgg ctacttccat cagtatggcg tagccggttt gtgcttctat ttctttcagt
     7561 ttgtcgagca gggctttttc ggtgatgtcc tgcatatcca cgtctattgt ggatactata
     7621 tccattccgt caaccggttc tacctgggtg atttcttccc atcggttggc caccttttgt
     7681 ctggacgata agcccggcgt acctttcaga atggagtcga atcccagttc aagtccgttt
     7741 ttgccacccc gtgattcgtc ggcataaata tcgccaatgg tgcgtgaggc cagacttgta
     7801 aatggtttta cccggctgaa catttctttg gtgagcaatc cgcttttgtt tctgcctaat
     7861 ctgaacagcg gaaaacgacg aatgtccttc aactgtgcgt aagagatttt tcctttgtag
     7921 aagttgtatt caccttttcc ctgtctgtaa gctgcagtca tgtctctttt gtaagcttcg
     7981 gacgatttgt cgccgaaata atcggacaga catatagaaa gtgagtcgat gttttcggca
     8041 aagagttttc cgtcatcttc atgaagtgct ggcacgcggg tatccatata tacgtagtag
     8101 gtgggtatgg agcttgccat gagtcttccg tcggcagagt agatattgcc tctgttggga
     8161 cgaacgatga tgtcgttttt gttgtttttt tcaccgagtt taagccagtt ctcgcgctcc
     8221 acagtctgaa ttttggctat gttgtagata atagacatca tcagtattac aattataatg
     8281 taaactgttg caaatcgtcc tattattttt tttccgggtt cagtcatttt ttttactctt
     8341 cgatgtgcgt ggagtcgctt tttactttta cagttgcagc tttgtgggct tctttttgtt
     8401 tttttacttc ttcatctttt accggatcag gttcttcgat tacaacaggt gcataagggc
     8461 tttctttcag gttcagtcct ttggcttcga tgtagtcaag aacataggtt cggcggctca
     8521 gttttgtcag ttcggccgaa atggtcagtg attcgaattt tacatcctga agttctttct
     8581 tcagttttac ttctttcgat acctgttttt cgcagtaata ccggttgtcc acgtaaaaaa
     8641 atgtcagaac tgcaatgagc agaaataaac cgtactgttt tttaatgaaa ttttttgaca
     8701 gaatattccc gttcagcaca tcgcggaagg tactcgactt taagtccgag aaatcctcgt
     8761 tacctttcac cacttccact attttattcc aaaaactcaa tttttttcgg ttgattgaat
     8821 gtttcaaatt tacgtagtaa tctgtttgtc agtttttcag cttttctgca attctcagtt
     8881 ttgcgcttct tgctctcgga tttcgggctg tttcttcttc cgatgcagtg attaccttgt
     8941 tgttgacggc tttgagcggt gcaatgatat tgccgtagaa atcttttacc tgtttgcctt
     9001 caaaattgcc gctgcgtata aagtttttta ccagtctgtc ctccaatgaa tgatatgtca
     9061 aaaccaccaa tctgccgttt tcgttcaagg tttctacgct ttgcgaaagc aattcctgca
     9121 gcactttcat ttccttattc acctctattc gcagtgcctg aaaaactttt gccatctctt
     9181 ttttctctct ttcgcgcggg aaaaacggtt tcaatacttc cagaaattgc tcgatactct
     9241 caattcttcc catgcttctc gcttttgcaa tggcagatgc aattctgcgc gaattgtgca
     9301 tttcaccgta aagataaaat acgtctgcaa gctgttcttc gctgtactcg ttgaccactc
     9361 ttgcagctgt aaacgatgag cgggtattca tccgcatatc cagttttcct tcaaaacgga
     9421 acgaaaaacc gcgttcggct tcatcaaaat ggtgaaacga gacgcccaag tccgctaaaa
     9481 ttccgtccac tttttccacg ccgtggtatt tcagaaaatt agtcaggtac ctgaaattgc
     9541 ttctcacgaa ggtaaatcgc tcatcatcaa tcatattttg caatgcgtcc tcatcctggt
     9601 caaatccgac taatcgccct ttagctccca gatgcttcaa aatctcacgg gagtgaccgc
     9661 tgccaccgaa agttacatcc acatatactc cgtccggttt tatgtttagt ccttcaatag
     9721 tttcagcgag cagggcaggg atgtggtaag ctcccccgtc actgccccct aaccccctga
     9781 agggggaatt tggtagtaat gattcagact tgatgtgttt ttgggaatta ttcactttct
     9841 cactctttaa tttccccctt taaggggctg ttttcatttt gtcttttaat ctctttgcaa
     9901 aatccccggc cggcaatttc acctcttcat atcgctggcg tccccatacg gcaaaacggt
     9961 ccatcattcc cataaacatg acttcattgt tttcgatacc gatgaggtcg aggtattttt
    10021 tagaaataag cactctgccc tgcgagtcga tatcaagcca ctctgcgtcc gatacgaatt
    10081 gcatcagcag cagctggtcg ccggcatccc attcgtccag attcaacttt aattcttcaa
    10141 tttttttatt ccacacagat tcaggataaa gtaccaggca gtcattgtcg gcatctttac
    10201 gcatcacaag ccggtctttt tctccgtccc ggagaacttt cctgtaaacg gacggaatga
    10261 atatgcgtcc ctttacatcg gcttttgcat cgtatttacc tatgaatgtt gacatgtttt
    10321 tatttaataa atttttccaa cggtaaaaat aaatcttttt tttcatattc cccacttttc
    10381 cccacaaata tttatcaaca aagttatcaa caagttatca ccttatattt agctatttat
    10441 taacaggttt tcaaaaaact attctaagaa tatgaattag taatttgatt gatatcagca
    10501 tattaagttt gtcttgttga taaattgcat gaaaaggatt agaagtgggg gagattttgc
    10561 tgtgcgtaga atgcaaatct taagcagaaa gaacaactga aatacccgga ataagataag
    10621 atttcgtatt tttgcacttt cacaattcga aaaaggaaat tttatggaag aaaaagtgat
    10681 gcgttttgat gtggaggaaa taatcagagc gaaagctccc gaaaaaaagt ttccgaaatt
    10741 tattgtcaac tatcttaaaa aaattttaca tcaggatgaa ctgaatgagt tttttgtgcg
    10801 tgctcacggc aaaaaaaatg tagagtttat tgattttgcc ctgggcgaag gtttgcttcg
    10861 ggttacagca gattttgagg gtgttgagaa tttgccaacg gatggaaacc gatatatttt
    10921 cgtgagcaat catccgctgg gagggcttga cagtgtaatt ctcgggcttc tgctgggccg
    10981 aaaatacgag ggcaaagtga agtattttgc caatgatatt ctgatgtttc tggaaccgat
    11041 gaaagatatg tttttgcctg tgagcaaggt gggtaaggct tcggatgtaa gagaaaacgc
    11101 caaagcggta gaggcttttt tcaattcgga caaccagctg attacttttc cggcgggtac
    11161 gtgttcgcgc aaagtgcacg gaaaaattca ggatttaccc tggaaaaaat cgttcgtatc
    11221 caaagcggtg cagtcaaaac gggatgttgt gcctattttt ttcaatggca gaaattcaaa
    11281 ctttttctac aacctgtcca ttatccgtaa taagctgggg ttgcccaaca ttgaaatgct
    11341 ctatctggta aacgaaatgt tcaaacaaca tgggaggcat tttaaaatta aaatcggcaa
    11401 acccatacct tatgccacgt ttgacaaaag taagacgcct gtagaatggg cggaatgggt
    11461 gaggagcaag gtgtatgaat tggaaaaaca aattaagtag ctgttacttg aaagtaactg
    11521 ctacttaatt tcgccaaatt ccgcgatatt atcatccaca taccgtgcaa ttatcgctgt
    11581 ctgttcttct tctatttcaa aaaaccgttc ttccagctca tcaaactgct caaaatccac
    11641 gtacatggct gtaaactctt cttcggtggt ttcccgctcc agttcggcgc ggtttgcatc
    11701 gtaaatttcc ctggctttgt aaatgatttt tgccaggtca gacgcaccca tttgtttcag
    11761 cgctttggcg gtcggattgt cgaaaatata actgccgtag ccgttttgga taagctgaac
    11821 aaaacctccg tacatcactt cttcgcgtag aaaatgaaat gccagcagcg tgtgctgatg
    11881 gccgttgagc aagtgcatat tttctgcatt caggtttcca cccaatgctt ccagataggc
    11941 atcggtaaat acctgcagga attcattcac gccgattgcg cctgcttgtt gtaggtttgt
    12001 gttggatatt tttatcataa agcctcccaa ccccctaaag aggggatttt gtagttgaat
    12061 tgattttttt aaaaaatatt atttttgctt tttttatgtt aaatagacag agttttgcgc
    12121 tccttttccc cctttagggg gttagggggc taaaacctcc gaactaaacg gctctaaact
    12181 tacctttaat tttccacctt tcacagtaaa tttacttccg aaactggctt tgaaatttcc
    12241 ttgtatattc gaggggattt ccacttccat ctctttgggc tcgttgctgt tgtttatcaa
    12301 cacaatagct gtttt
//
LOCUS       DAORKP010000060        12193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_512_length_12193_cov_7.673917, whole genome shotgun sequence.
ACCESSION   DAORKP010000060 DAORKP010000000
VERSION     DAORKP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_12193_cov_7.673917"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLN75_04825"
     CDS             complement(<1..1254)
                     /locus_tag="PLN75_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HRG03474.1"
                     /translation="MKLKYFLILFITLVTLSFAGCKEDEPKPDGEKPNPVPSFTIPTY
                     ADDYSSISSWTNRNKWNLANVHDPSVAFYKGYYYMYGTDASYGNAHEGHGHFQGKRST
                     DLVNWDWVGGPFYDPPAWVADSLNAIRTRMGLPVIAGDKIQYGYWAPVVRRVNVGGQE
                     ILRMYYSIIIDNYIKTGKAATSANFDGSWGERAFIGMCESTDPAGAVWTDRGFVTCSS
                     SDRGLNYNRASTNDWSAYFYYNAIDPTYIVSPDGKHYLIHGSWHSGFALLQLNATTGK
                     PLNTLGNPWANNAGELASRYGVRIGTRTANSRWQGSEAPEIIYKDGYYYLFMAYDGLD
                     VPYNTRVVRSATIEGPYLDITGRNFTAGAGDSYPIVTHPYKFNLSNGWVGISHCAVFK
                     KENTNEWFYMSQGRLPVNVGGNAYSN"
     gene            complement(1345..3099)
                     /locus_tag="PLN75_04830"
     CDS             complement(1345..3099)
                     /locus_tag="PLN75_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03475.1"
                     /translation="MKLINKILTILVLTGGMTACVDQLDVQNPNNQTTYDFGKTESDL
                     QEAVISCYNRIRLEGTFARVGYTLDAVRGDEVWNSSQQWYLEYDNLNSPGTIGIGDEW
                     PWRDNYHVVNRTNFVLSKVDGVKMSETSYNEIAGQALFLRSLAYYHLATYYQNVPLIT
                     DYASYSDINTMYAANATQDQVFDQIETDLKKAMEMLPSRDKGGEWAKGRATSGAAAGY
                     LARALMFRHKFTEAYAVLKDIIAGKYGRYELTKDFGDNFREGPAYENNSESLFEVQFL
                     DFGTGGTDEEWTPVNISPEATQGHAVESNYASQELGSWGDLAGSPWLYNLFRKENSTD
                     GRLDPRLYWTLVTFEPEYNTYTGQKTAAYPGGDPRSNTIYQKDITASPLSNNAQGGIS
                     IAKFTNARNNIYSSINNGLHCGINLRLMRYSDVLLRAAECENEISGPTQTAIDYINTV
                     RRRVALKDLKLSDFPTADALFEQIANVERPKEFGCENGRGIDLLRWGFFYDSDRLNQL
                     VEHGYYKRDGKASTELLTAETADDSSFKYYYKGHEYLPIFQSTLNANPNLVGNSANNN
                     EDNGPAFKAKWTVRPVVK"
     gene            complement(3131..6331)
                     /locus_tag="PLN75_04835"
     CDS             complement(3131..6331)
                     /locus_tag="PLN75_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03476.1"
                     /translation="MKYSYLILIGLLALAPATYAEGNGNADQLSMNQTNKITVKGKIV
                     DDKGEPLVGATVQQKGTTVGAITDFNGNFSLSVPSDASLVVSYVGCLPQEIFVGGKSD
                     FGIITLASDVKELEQVVVIGYGTQRKVDLTGSVAIVDAEEMKKVSHSNISTMLEGKVA
                     GVQITTDGQPGADPIVRIRGIGSFGSTAPLYIVDGVPMGTTIRDFSPNDIETLQVLKD
                     ASAAAIYGSRAANGVVIITTKQGKKNQTMKIDYSGYVGYDQVQKGVYDVMDSYQYGQY
                     VNMAYSNSGMDVPAGYNPASPKYVDPATVNTNWYNEAFKTGVRQNHNINMSGGSANNT
                     YNIGLDYFTQKGTMVGAGPNFDRLTARINNTMDVKFVKFRTNVVYSHSNQDNMALSNA
                     NEYVQGLYGAQFPVMASVLLLPPSIRAYDPSTWVLDDKISAASQYSYDSYGYGTYYDD
                     VHGDIRVTNVLLENSLIKRNAKVDRIVASGSSTVDLIKMVGLSSANHKLDYNLNLSYS
                     KTFVKDLTFVPAFIQSTTNYLAKSNENLAQGYRSYSDALIENTITYDGKFGLSHFNVV
                     AGQTFERELFHTLTGKGVTMPEPYYLQVNNAEETSAGSFESEHVLASYIGRINYDFDQ
                     KYLISATARRDGSSRLSSADRWDWFPSVSAGWRIERENFFPEAAKSKINLLKLRGSYG
                     VLGNENIGEYQYMDVMPRGNYTYSFGNTKVTGSAISNYVNTAIRWEKKKTLDFGLDLG
                     MFNNQFEFTFDWYKSTSEDLLYSVAVPTNAGATNGTVTMNAATMENSGLEFLAAYHNH
                     KNAVKFDISANLSTLSNKVIKLGVSGEPRTDGYCRTEVGREVGSFYGYVYEGIFQSQS
                     EIDNRVNSDGKLINQPGAQPGDVAYADLDLDGEITNKDQTYLGSGIPKIHYGLNARAE
                     WKGFDLSVSTYGAAGFKAVDFVDVALRSSYGALNKDVRLLNAWTPENTNTDVPRIAYK
                     STGSITNDMFSERYIQDASYLKIANIELGYNFSDKLFGNYVNGVRIYASAQNIATLSK
                     YIGYNVDFAGGTFTPGYNYASYPTPRTIMFGARFSF"
     gene            complement(6455..8146)
                     /locus_tag="PLN75_04840"
     CDS             complement(6455..8146)
                     /locus_tag="PLN75_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03477.1"
                     /translation="MKTTKLLRSALYATLLAAIGLVGCERLDLYSIDAPSDLQSRIDS
                     IAAARPNTGDTTYINITTAIVGPEDNSAAWWAHFSDYFTIPTNKLLHMEFVNHGSGVN
                     NWNNWNLAITNEVANRTGDGYKEYFILRSDAYGWGNTDFNLKMITHNYPDTDGDGDIW
                     NDFRRTMQGARVALDVDHSATGNVFVTATAVGTNGVTLVETYQQPVSATADIVAFLIA
                     DGSYFEMKKAYLIPSKVTAVEDVEPVSITVSGAPTFVELGNNNFWGDATATVTFADGS
                     TAKVDSADLSFNVIPDMTTIGMKTVVVAYNKTKQGEYTKAVSTIYKLEVTNPVVSLEV
                     TTMPLITTYYYFNADPIIFNTTGLVVTATYSDGTKTIIPNSSLQFGKIPAATGSQGVL
                     ISYAGATKTVSTTCPLTLVKGIGQVGNTDFSSGWWTAFSPDYKVASGASKTITMYCYS
                     DNLANWHSPCTILRKTDKTENAVVRMDSFGWGAGYGTAILTSDWNWDKFTSSINGSRV
                     AITVKNNGNNTADVSYDVTYATGEKHFQKYAGITVDSSDLNCALVTEVSYLVIVE"
     gene            complement(8537..10018)
                     /locus_tag="PLN75_04845"
     CDS             complement(8537..10018)
                     /locus_tag="PLN75_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HRG03478.1"
                     /translation="MKKAIYILMVCCLPAFTACSYKSFEPTQSSNPWKDDYRSVSGMK
                     DYMKWGTYNVHDPSCKLFGDTFYMYSTDAIFGENKKEIQEKKLPFGYIQVRKSKDLVH
                     WEFAGWAFREIPQEAKNWVLNNSDGRGATNIWAPYILQYNGKYRLYYCVSAFGKQTSY
                     IGLAESESPEGPWTLKGCVVKTKTGDKMNAIDPSVVTNPETGEQWMHYGSYFGGLYCV
                     KLNPETGLTMQDGDQGHLIARRFNGKKNNIEAPEVIYNPDLKKYFLFVSYDPLMTTYN
                     IRVGRSDKPEGPFMDYNGKDMAEEEDNFPIITYPYQFNGHKGWAGTGHNTVFSDVNGD
                     YYLASQARLSPENHLMDLHVRQVFWTKDGWPVVSPERYAATKQMGIGEKDIHGDWEVI
                     TIHGDAPLRELEAGQILWGENLLKPEEVNRSAVVTLHKNKTISGEISGNWTYESGILK
                     LNTDNKIVELKVFTGQDWENEKTTLLFTGLNDRGYSIWGKRIK"
     gene            complement(10033..11574)
                     /locus_tag="PLN75_04850"
     CDS             complement(10033..11574)
                     /locus_tag="PLN75_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HRG03479.1"
                     /translation="MKHKLFFPFMLMLAFQLSAQQKTEVTIRTDKGEYRINKHIYGHF
                     SEHLGRGIYGGLWVGENSTIPNVNGVRKDIIDALKHIHIPNLRWPGGCFADEYHWMDG
                     IGPRSERPKMVNTHWGGVVEDNSFGTHEFLNLCEALETEPYICGNLGSGTVEEMSKWV
                     EYITFGGESPMANLRKKNGREKPWKVSLWGVGNENWGCGGNMTPETYAENYRRYATFC
                     RNYGDNKLYKIAGGPNVDDYRWMDVMMRNIPHFLMNGVSVHNYTFTRAWEDKGNATGF
                     SEDDYFSLLQHGLRMDEIVTKHAEVMDKYDPAKRIGMIVDEWGAWYNVEPGTNPGFLY
                     QQNTLRDAILAGTILNIFHKHADRVQVANIAQLVNVLQALFLTEGDKMLLTPTYHVFD
                     MYKVHQDATMLPVEFTSPTYSRLGKSIPSLSVSASRDKNGAVHVSFVNIDPTQAVEIS
                     SNMTDVKGKLTSAGIITAPKTDSYNTFENPNQVVLKEYKNGSFKNGILKIRIPEKSVV
                     TIEIK"
     gene            11958..>12193
                     /locus_tag="PLN75_04855"
     CDS             11958..>12193
                     /locus_tag="PLN75_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRG03480.1"
                     /translation="MKKHVFLYLCILIFAPVLHSQNSVVSKYIFNSLTVAESLPHNFI
                     DDLYKDNQGFIWISTGGGGLVRYDGYEFLTLNVN"
BASE COUNT         3021 a         2706 c         2768 g         3698 t
ORIGIN      
        1 gttcgaatag gcattcccac ctacatttac gggcaatcgt ccctgcgaca tgtaaaacca
       61 ttcgttggta ttttcttttt taaaaactgc acagtgtgaa attcctaccc atccgttgct
      121 aagattaaat ttgtatgggt gtgttacaat ggggtagctg tcgccggcac cagctgtaaa
      181 gttacgtccc gtgatatcaa ggtatggacc ttcgatggtg gcgcttctga ccacccgcgt
      241 gttgtatggc acatcaagcc catcatacgc cataaacagg tagtagtaac catccttata
      301 aataatttcg ggagcttcgc tgccctgcca gcgcgagtta gcagtgcgag tacctattct
      361 gacaccgtag cgggaagcta actcaccggc attgtttgcc cagggattgc cgagtgtgtt
      421 gagtggcttg cctgtagttg cattgagttg caacagtgca aaaccactgt gccacgagcc
      481 gtgaatcagg tagtgtttcc catcggggct tacgatatac gtcgggtcga tggcattgta
      541 gtagaaatag gcgctccagt cattcgtact tgcgcgattg tagttgagac cacgatctga
      601 tgacgaacag gtaacaaaac ccctgtctgt ccataccgct ccggcgggat cagttgattc
      661 acacatgccg ataaaggcgc gttcgcccca gcttccgtca aagttggcag aggttgcggc
      721 tttacccgtt ttaatataat tgtcgatgat aatactgtag tacattcgca gaatttcctg
      781 tccacccacg ttaacccggc gaactaccgg tgcccagtag ccgtattgaa ttttgtcacc
      841 cgctatgaca ggaagcccca tgcgagtacg gatggcgttg agcgaatcgg ctacccaggc
      901 aggcggatca taaaagggtc cgcctaccca atcccagttc accagatcag ttgaacgttt
      961 accctgaaaa tgaccatgcc cttcgtgtgc attaccatac gaggcatcgg tgccgtacat
     1021 gtagtaataa cccttgtaaa acgcgaccga cgggtcgtgt acattcgcca gattccactt
     1081 gtttcggttt gtccacgatg atatcgaaga ataatcgtcg gcgtatgtcg gaatggtgaa
     1141 agaaggaact ggattaggtt tttcaccgtc aggtttgggt tcatcttctt tacagcctgc
     1201 aaatgataac gtaactaagg taataaataa tattagaaaa tacttcagtt tcatattaaa
     1261 tgacttttgt tttaagaaaa cttccttatc gagaagtaca aaaaatatgc cgataaggaa
     1321 gtctccacac catgagaaag tgttttattt taccacagga cgtaccgtcc atttagcttt
     1381 gaaagcaggc ccgttatctt cattattgtt tgccgaattg cccaccagat tggggttagc
     1441 attcagtgta gattgaaata tcgggagata ttcatgtcct ttgtaatagt atttgaacga
     1501 tgaatcgtcg gcagtttcgg cagtaagtag ctcggtagaa gcttttccat cgcgcttgta
     1561 atagccatgt tcaaccagct gattcaaacg atctgaatcg tagaaaaatc cccaacggag
     1621 caggtcaatt ccacggccgt tttcacaacc gaattctttc ggacgttcaa cgttggctat
     1681 ttgttcaaaa agagcatcag ctgtcgggaa gtcggacagt ttaagatcct taagagctac
     1741 acgcctgcgt acggtgttga tataatcgat agcagtctgg gttgggccac tgatttcatt
     1801 ttcgcactct gctgcacgca gcaacacatc gctgtaacgc atcagtcgca ggttgatgcc
     1861 acagtgcaat ccgttgttga tggaactgta gatattgttt ctggcatttg tgaacttagc
     1921 tattgatatt ccaccctgag cattattcga cagaggagaa gcagttatgt ctttctgata
     1981 aatggtattg ctgcgtggat ctccgccagg atatgctgcc gttttctggc ctgtataggt
     2041 attgtattcc ggttcgaacg taaccagtgt ccagtacaaa cgggggtcaa gacggccatc
     2101 ggtagagttc tcttttctga acaggttata caaccaggga gaaccggcaa ggtcgcccca
     2161 gctgcccaat tcctgtgaag cataattgct ttccacagcg tgaccctgtg tggcttccgg
     2221 gctgatatta accggagtcc attcttcgtc ggtaccgcca gtgccaaagt ccaggaactg
     2281 cacttcaaaa aggctctcgg agttgttttc gtatgccgga ccttctctga agttgtcacc
     2341 gaagtcttta gtaagttcat aacgtccata cttgccggcg atgatgtctt tcagtacggc
     2401 ataagcttcg gtaaatttat gacggaacat cagtgcacgt gccaggtatc cggcagcagc
     2461 accgcttgtt gcacgacctt tagcccattc tccgccttta tcgcgcgagg gaagcatttc
     2521 catcgctttt ttgaggtcgg tctcaatctg gtcaaatacc tgatcctgag tagcgttggc
     2581 cgcatacatg gtattgatat ctgaatagga ggcataatcg gtaatcaacg gaacattctg
     2641 gtaataggtt gccagatgat aatatgccag agaccggagg aagagagcct gtccggctat
     2701 ctcgttgtat gaagtttccg acatttttac accgtccact tttgacagta caaagtttgt
     2761 acggtttacc acgtggtaat tgtcacgcca tggccattcg tcacctattc caatggtacc
     2821 cggagaattg aggttatcat attccagata ccactgttgt gaggagttcc acacttcgtc
     2881 gccgcgtaca gcatccagcg tgtatccgac acgggcaaaa gtaccttcga gacgtatacg
     2941 gttgtaacac gagatgacag cttcctgcag atctgattct gttttgccga aatcataggt
     3001 tgtctgattg ttcgggttct gaacatcaag ctggtcaacg caggcagtca tgcctcccgt
     3061 aagcaccagt attgttagaa ttttatttat aagtttcata ttgctgttct attattaaaa
     3121 ttttgttttt ttagaatgag aagcgtgcac cgaacatgat ggttcgtggt gtaggatacg
     3181 atgcatagtt gtaacccgga gtaaatgttc ctcccgcaaa gtcaacgttg tagccgatgt
     3241 attttgacag tgtggctata ttctgagcag aagcgtaaat ccggacaccg tttacatagt
     3301 taccaaacaa tttgtctgaa aagttgtaac ccaattcgat gttggctatt ttcaaatagg
     3361 atgcatcctg gatatatcgt tcgctgaaca tatcgttggt gattgatccg gtggatttat
     3421 aagctattcg gggcacatcg gtgtttgtgt tttccggtgt ccaggcattc agaagcctta
     3481 catccttgtt gagtgcgccg tacgagctgc gcagtgcaac gtcaacaaaa tctaccgctt
     3541 tgaaacctgc agctccgtag gtcgaaacgc tcaggtcaaa tcctttccat tcggcacgtg
     3601 cattcaatcc gtaatgaatt tttggaatac cacttccaag gtatgtctgg tccttattgg
     3661 ttatttcgcc gtcgagatca aggtctgcgt atgccacatc accgggctga gctccgggct
     3721 ggttaataag tttgccgtct gagttcacgc ggttgtcgat ttcggattgc gactggaaga
     3781 taccttcgta cacataaccg tagaatgaac cgacttcacg ccctacctct gtgcggcaat
     3841 agccatcggt gcggggttcg cctgaaacgc ccagcttaat cactttgttg ctgagggttg
     3901 ataagtttgc agagatatca aatttaactg cgtttttatg attgtggtaa gcggccagga
     3961 attcaagacc agaattttcc atagttgcag cgttcattgt tactgttccg tttgtagcac
     4021 cggcattcgt aggaactgca acgctgtaaa gcaggtcttc tgatgttgac ttataccagt
     4081 cgaatgtgaa ttcgaactga ttgttgaaca ttccgaggtc gagaccaaag tccagtgttt
     4141 tcttcttttc ccatctgata gccgtgttta catagtttga tatggctgag ccggtaactt
     4201 tggtatttcc aaaactatag gtatagttgc ctcgcggcat aacatccatg tactggtatt
     4261 caccaatatt ctcattaccc agtacgccgt agcttccacg cagcttcagc aggttgattt
     4321 ttgatttggc agcttcagga aagaagtttt cacgttcgat acgccatcct gccgatactg
     4381 atgggaacca gtcccaacgg tcggctgaag aaaggcggct tgaaccatcg cgacgggcag
     4441 tagctgagat aaggtatttc tgatcgaaat cgtagttgat acgtccgatg taggatgcca
     4501 acacgtgttc actttcaaag cttcccgctg atgtttcttc agcattgtta acctgcagat
     4561 agtatggttc gggcatggta acgccttttc cagtaagagt gtgaaagagt tcgcgctcaa
     4621 atgtctgacc ggcaaccaca ttgaagtgac tgagtccgaa tttgccatca taggtaatcg
     4681 tattttcaat aagcgcatcg ctatagctgc gatacccctg agccagattt tcattgcttt
     4741 tagccaggta gtttgttgta ctctgaataa atgcaggcac aaacgtaagg tcttttacaa
     4801 atgtcttgct gtacgacagg ttgaggttgt aatccagctt gtgattggcg ctgctgaggc
     4861 caaccatttt tatcaggtcc acagttgaag aaccggatgc tacaattcgg tcgacctttg
     4921 cattcctctt tattaaacta ttttcgagca gaacgttcgt tacacgaatg tctccatgaa
     4981 cgtcgtcgta gtaagttcca tatccataag agtcatagct atactgtgat gcagccgaaa
     5041 tcttatcgtc gagcacccag gtagaagggt cgtaagccct gatagacggt ggtaaaagca
     5101 aaaccgaagc cattacggga aattgagcac catacaggcc ctgtacatac tcattggcat
     5161 tactgagtgc catattgtcc tggttgctat ggctgtatac cacgttggtt ctgaatttaa
     5221 caaatttcac atccattgta ttgttgatgc gggctgtgag acggtcgaaa ttaggaccgg
     5281 cacctaccat ggtccctttc tgtgtaaagt aatcgaggcc aatgttatag gtattgttgg
     5341 cactgccacc cgacatgttg atgttgtgat tctgacgaac gccggttttg aacgcctcgt
     5401 tgtaccagtt tgtattaact gtggccggat ctacatactt cgggcttgcg ggattgtatc
     5461 cggcaggcac atccattccg ctgttgctgt aagccatgtt cacgtattgg ccgtactggt
     5521 atgaatccat tacatcgtaa acaccttttt gcacctggtc gtagccaacg taaccgctgt
     5581 aatctatttt catggtctgg ttcttcttgc cctgcttggt ggtgatgata actacaccat
     5641 tggcagcacg cgaaccatag atagcagctg cagaagcatc tttaagtacc tgtagggttt
     5701 ctatatcgtt cggtgagaag tcgcgtatgg ttgttcccat aggcactcca tcaacaatat
     5761 agagcggtgc ggtactcccg aacgaaccga taccgcgaat acgtactata ggatcagctc
     5821 cgggctgtcc gtcggttgta atctgaacac ctgccacttt tccttcaagc atggttgaga
     5881 tgtttgaatg tgaaactttt ttcatctctt cggcgtcaac aatggcaacc gaaccggtaa
     5941 gatctacttt acgttgtgtg ccgtaaccta ttaccaccac ctgttcgagt tcttttacgt
     6001 cagaagctaa agtaatgata ccaaaatcgg attttcctcc aacgaagatt tcctgaggta
     6061 agcatcccac ataagatacc acaagcgaag catctgaagg aacagaaaga gagaagttac
     6121 cattgaagtc ggtaattgca cctacagttg tacctttttg ttgtacagtt gcacctacaa
     6181 gcggttcacc tttatcgtcc acaattttcc cttttacggt aattttattt gtctgattca
     6241 ttgacagctg gtcggcattg ccgttgcctt ctgcgtatgt agccggtgcg agcgccagca
     6301 atcctatcag gattaaatat gaatatttca tctgtttata ttttaatttc ttaacactaa
     6361 tgtcagatgg aacacgccat acaatcatta ctttgtgtgt caaaagtatt ttggcatggc
     6421 tgtttcatgg ttaaattatt tattatctta ttgattattc tactattacg agataggaca
     6481 cctctgtaac tagtgcgcaa ttcagatcag aactgtctac cgttatgcct gcatatttct
     6541 ggaaatgttt ttcgccggta gcgtaagtca catcgtaaga aacatcggca gtattgttac
     6601 cattattttt tacagtgatg gcaaccctgg atccgttgat gctggatgtg aatttatccc
     6661 agttccagtc tgatgtgagg atggctgtac catagccggc accccagccg aagctgtcca
     6721 tgcgaactac tgcattttca gtcttgtcgg ttttgcgtag tatggtacat gggctgtgcc
     6781 agttggccag gttatcggag tagcagtaca tggtgattgt tttgcttgca ccggatgcta
     6841 ctttgtaatc gggtgagaat gctgtccacc atccggatga gaagtcggta tttcctacct
     6901 gacctatacc ttttacaagt gtgagtgggc aggttgtcga taccgttttc gttgctccgg
     6961 catacgaaat gagtactccc tgcgaacccg ttgcagccgg aatcttgcca aactgaagcg
     7021 atgagtttgg aatgatggtt tttgtaccgt cggagtaggt tgctgtaaca accaggccgg
     7081 tcgtgttgaa aatgatggga tcggcgttga aataatagta ggtggtgatg agcggcatgg
     7141 tagtgacttc gaggcttact accggattgg taacttccag cttgtagatg gttgatacag
     7201 ccttggtata ttcgccctgc tttgttttgt tgtaagctac aactacagtt ttcataccga
     7261 ttgtggtcat atcaggaatg acgttgaatg ataaatcagc agaatcgacc tttgcggtgg
     7321 agccgtcggc aaaagtaaca gtagcggtgg catcacccca gaagttattg tttcctaatt
     7381 caacaaacgt gggagctcca ctgacagtaa tcgaaaccgg ctctacatct tccactgcgg
     7441 tgactttaga aggtatgagg tatgcctttt tcatttcgaa atagcttccg tcggcaatta
     7501 aaaaagccac aatatcggcg gtggcagaaa ccggttgctg ataggtttct accagcgtaa
     7561 ccccgtttgt gcctactgca gtggcagtta caaacacatt accggtagcg gagtggtcca
     7621 catcgagggc aacccgtgcg ccctgcatgg ttctacggaa gtcgttccag atatcaccat
     7681 ccccatcggt gtcgggataa ttgtgagtaa tcatttttaa gttgaaatcc gtatttcccc
     7741 agccgtaagc atccgaacgg agtatgaaat attctttgta tccgtcgcct gtacgatttg
     7801 ctacttcgtt ggttattgcc aggttccagt tgttccagtt gttaactccc gaaccatggt
     7861 tcacaaactc catgtgcagc agtttgtttg ttggaatagt gaagtaatct gagaaatgag
     7921 cccaccaggc agcactgtta tcttcaggtc ctactattgc tgtagtgatg ttgatgtagg
     7981 tagtgtcacc tgtattcggt ctcgcagctg caattgagtc gatcctgctt tgcaggtcgg
     8041 agggtgcatc aattgaataa agatccagtc gctcgcaccc aactaatcca attgctgcca
     8101 aaagagttgc gtacaatgcg cttctcaata gtttagttgt tttcatacat ttaaaattaa
     8161 ttgaattctt tctatgatat ttttaaaaat cctggtctgc agttttgtca actgcggatt
     8221 gttttattta cttgaaaagt agattttttt tgtgttttca atgttgttcc ggaagtgaat
     8281 ctatattagc tcgcggttat gatgagtttt agttaataat atattttctt ccattcacac
     8341 caatgatttg atctgtattt atcaaactgg cgaaatcaac ttttttattc agggcggtcc
     8401 tttaatggaa tcggcgccaa tctgctgatc aaatttgtct gtctcaaagc atgattctat
     8461 cccggcaaac aaattcgaca attgtgttaa ctttttcatt ttgtaaaaga atttgagttc
     8521 gatttattga tacaatttat ttgattcttt ttccccagat ggaatatcct ctgtcattga
     8581 gtccggtaaa gagcaatgtt gtcttttcat tttcccagtc ctgtcctgta aacaccttaa
     8641 gttcgactat tttattgtct gtattaagtt tcaaaattcc gctttcgtat gtccagttgc
     8701 cggatatttc accgcttatt gttttgtttt tgtgtagtgt gaccacagcg gaacgattta
     8761 cctcttcggg tttcaaaaga ttctctcccc agagtatctg tccggcttcc agttccctga
     8821 gcggtgcatc accgtgaatt gtaatcactt cccaatcgcc gtgaatgtct ttttcaccga
     8881 tgcccatttg ctttgtggcg gcatatcgct caggcgacac caccggccaa ccgtctttgg
     8941 tccagaatac ctgcctaacg tgcaggtcca tcaggtggtt ctccggactc aaacgtgcct
     9001 gacttgccaa ataataatct ccgtttacat cactgaaaac agtgttgtgg ccggttccgg
     9061 cccacccttt atgcccgttg aactgatatg gataagtaat aattggaaaa ttatcttctt
     9121 cttcagccat atctttcccg ttgtagtcca tgaaaggtcc ttccggtttg tccgaacgac
     9181 cgacacggat attgtaagta gtcatcagcg ggtcgtagga aacaaaaaga aaatactttt
     9241 tcagatcggg attgtaaatg acttcaggag cttcgatatt gtttttcttt ccgttgaaac
     9301 ggcgggcaat cagatgtccc tggtcgccat cctgcatcgt gagtcctgtt tccggattca
     9361 gcttcacgca atacaaacca ccgaaatacg acccgtagtg catccattgt tcacctgttt
     9421 cagggtttgt tacaacactt ggatcaatgg cgttcatctt atctccggtt ttggttttca
     9481 ccacacatcc cttcagcgtc cagggtcctt cgggtgactc cgattcggca aggcctatgt
     9541 acgatgtttg tttgccgaat gccgaaacac agtaatataa tctgtatttc ccgttgtact
     9601 gcaaaatgta aggcgcccaa atattggttg cacctcttcc gtcgctattg ttgagcaccc
     9661 aattttttgc ttcctgcgga atttcacgga acgcccagcc agcaaactcc cagtgaacca
     9721 ggtctttgga tttgcgtacc tgaatatagc cgaaaggcag ttttttttcc tgaatttctt
     9781 ttttattttc tccgaaaatg gcatcggtgg agtacatata aaaggtatct ccgaaaagtt
     9841 tacaggaagg gtcgtgaacg ttgtaagttc cccatttcat gtagtctttc ataccagaca
     9901 cgcttctgta atcgtctttc cagggatttg aactttgtgt cggttcaaaa gacttgtagc
     9961 tgcatgcagt aaatgccgga agacaacaaa ccatcagtat ataaatggct ttcttcatac
    10021 cgttcggatg tgttatttga tttctatagt aactactgat ttttcaggaa ttcttatttt
    10081 caggattccg tttttgaaac ttccgttttt atactctttg agcaccacct gattcgggtt
    10141 ttcaaaggtg ttgtaagagt ctgttttcgg agcagtaatg ataccggctg atgtcagttt
    10201 gcccttcaca tcagtcatat tagaggatat ctccactgcc tgagttgggt caatgttgac
    10261 aaatgataca tgtacagctc cgttcttatc tctcgaagcc gacaccgaaa gcgatggaat
    10321 ggatttgccc aggcggctgt aagtgggcga tgtgaattca acaggcagca tggtggcatc
    10381 ctgatgaact ttatacatat caaaaacgtg gtacgtaggt gtgagcagca ttttatcgcc
    10441 ttcagtaagg aacagcgcct gcagtacgtt gaccagctgt gctatattgg ctacctgaac
    10501 acggtcggca tgtttgtgaa aaatattcag aattgttcct gccagaatgg catcacgaag
    10561 agtgttttgc tgataaagaa aacccggatt cgttcccggt tctacgttgt accaggctcc
    10621 ccactcgtca acgatcattc cgatacgttt cgcggggtcg tatttatcca tcacttcggc
    10681 gtgtttggta acgatttcgt ccatgcgcaa gccgtgctgc aggagcgaga aatagtcatc
    10741 ttcggagaaa cctgttgcat tgcctttgtc ttcccaggca cgcgtaaatg tgtaattatg
    10801 gactgaaacg ccgttcataa ggaaatgagg gatattgcgc atcattacgt ccatccatct
    10861 gtaatcgtcc acgttgggac cgccggcaat tttatacaac ttgttgtcgc cgtaattccg
    10921 gcagaaggta gcatatcttc tgtagttctc agcatacgtt tcgggggtca tgtttccacc
    10981 gcagccccag ttttcgttgc ctactcccca caaacttacc ttccagggtt tttcccgtcc
    11041 gtttttcttt ctgaggtttg ccatggggct ttcaccgcca aaagtgatgt attccaccca
    11101 tttggacatt tcctccactg tgccgctacc gaggtttccg cagatgtagg gttctgtttc
    11161 gagggcttcg cagagattca gaaattcgtg ggtgccaaaa ctgttgtctt ccactactcc
    11221 accccagtga gtgttgacca ttttgggacg ttcggagcgg ggtccgatgc cgtccatcca
    11281 gtgatattcg tcggcaaaac aaccgccggg ccatcgaagg ttaggaatgt gaatgtgctt
    11341 cagggcatca atgatatctt tacgaactcc gtttacattc gggatagttg agttttcacc
    11401 cacccaaaga ccgccgtaaa ttcctcttcc aaggtgttcg gaaaaatgac cgtagatgtg
    11461 cttgttaata cggtattcgc ccttatcagt gcggattgta acttcggttt tctgctgtgc
    11521 cgatagctga aatgcaagca tcagcataaa aggaaaaaat aatttgtgtt tcatgatata
    11581 aataaataaa tttaccgttt taatgttttt tctgtctgta gccggttttt tcacaatagt
    11641 gaaaataatg aatcagagaa aaaaaccgga atcgatttga atgaaagcta agtggttatc
    11701 ggataaaacc gatgaataat attgcaaaaa acattatttt tgctgttata ataatattta
    11761 aggtaataaa gtaaattttt tgctgttttt ctctaaaaac agtgttttct gatttcaacc
    11821 tgatgcaaat atacttgaat tgttcagttg gcagggtgga catttctcct taaaaggtgg
    11881 acaattgtat ttttaagtgt aaaattcaca cttttcggtt ttttatggtt atttttgttt
    11941 ttgaaaactc aggcaaaatg aaaaaacacg tgttcctgta tctttgcatt ttgatttttg
    12001 cgcccgtatt gcacagtcaa aattcagtgg tatcaaaata tatcttcaac agtcttacgg
    12061 tggccgaatc tctgccgcac aattttatcg atgacctcta caaggataat cagggattta
    12121 tctggatttc gaccggcgga ggaggcctgg tacggtatga cggttacgag tttcttaccc
    12181 tgaatgtcaa cag
//
LOCUS       DAORKP010000061        12147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_522_length_12147_cov_8.180863, whole genome shotgun sequence.
ACCESSION   DAORKP010000061 DAORKP010000000
VERSION     DAORKP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_12147_cov_8.180863"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..738)
                     /locus_tag="PLN75_04860"
     CDS             complement(109..738)
                     /locus_tag="PLN75_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRG03481.1"
                     /translation="MTLTNSPFRGLGGRFSTAYLPPVSYFAALLNAENVWLEACENYV
                     KQSYRNRCRIATANGVETLSIPVESNSGRKNPIRDVRISSHGNWQQTHWRAISSAYMN
                     SPFFEYYQDDFRPFYENKWTFLWDYNLELLTLILQLLNENPKIILTEKYKEPTEDNID
                     FRDKIHPKKETISHIKPYYQVFENKFGFLPDLSIIDLLFNMGNEAVMML"
     gene            complement(815..2305)
                     /locus_tag="PLN75_04865"
     CDS             complement(815..2305)
                     /locus_tag="PLN75_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRG03482.1"
                     /translation="MTTQTNPQPASRFDKPTSQWIKAIIWCIVYILFVIWLGNYWWML
                     VLPLIFDAFITKFIPWTWWKNSKSKTVRSVMSWVDAIGFALIAVYLINNYLFQNYQIP
                     TSSLEKTLLVGDFLAVSKASYGPRVPMTPFSFPLVQNTFPILNSKSYLEKPQVKYRRL
                     KGHDSIERNDIVVFNFPAGDTIAVKAQNPDYYTLCYREGLAYVINYLQVEPTAELCYR
                     YGREIVKSKAAQYGEIMYRPVDRRENYVKRCVGIPGDTLQIINNQIVVNGTPQPKFPG
                     IQFNYFVQTDGTRLTREALDKMNISNEDFWEIDNAERFELLKTLGLNTNFPVYHFPLT
                     EETYKMMLNTRGVIKMVIEPDSAFVYHRNDDDVYPLGGGLKWTRDNYGPVYIPKKGST
                     IPLNKETYAIYERCIKNYEGNKTEVKNGQFFINDKPATSYTFQMDYYWMMGDNRHKSS
                     DSRYWGFVPEDHVVGRPMFVWLSLNKDKSLFNGKIRFERFFKNARK"
     gene            complement(2602..3318)
                     /gene="dapB"
                     /locus_tag="PLN75_04870"
     CDS             complement(2602..3318)
                     /gene="dapB"
                     /locus_tag="PLN75_04870"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRG03483.1"
                     /translation="MKIALIGYGKMGKIIERIALERGHEIVSVIDIDNREDFQSEAFR
                     SADVAIEFTTPHIALQNYDEAFRAGVAVVSGTTGWTEHLEEVKNNVEKNNHTLFWSSN
                     FSLGVNIFITLNSYLAKMMNQFPDYNVEMTEIHHTQKLDAPSGTAITLAEGILGNLDR
                     KMVWVKDTENNSDELAIKSIRDGEVPGIHTIRYESAVDSIEITHDAKSREGFALGAVV
                     AAEFTKGKKGFLGMGDLLKF"
     gene            complement(3429..3788)
                     /locus_tag="PLN75_04875"
     CDS             complement(3429..3788)
                     /locus_tag="PLN75_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRG03484.1"
                     /translation="MKKTLIIGASDNPDRYSYKAAERLLAHGHEIELLGLRPRKIFGR
                     IIDTERKNYADINTVTLYVGAQRQPEYYDYIVSLNPRRVIFNPGTENEEFYKVLKKNN
                     IEAEVACTLVLLGTGQY"
     gene            complement(3799..4848)
                     /gene="aroB"
                     /locus_tag="PLN75_04880"
     CDS             complement(3799..4848)
                     /gene="aroB"
                     /locus_tag="PLN75_04880"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRG03485.1"
                     /translation="MPAPNTIISTHIIRQLEYEICDWESDDIYILTDTNTREKCLPLL
                     FESEKIRKGHQITVQAGDAQKNIDSLQQIWRYLTENGATRKSLMINVGGGMITDMGGF
                     AAATFKRGIDYINVSTTLLGAVDAATGGKTGINFLGLKNEIGAFYPAKKVLINVEFFR
                     TLDDKNLRSGYAEMIKHALISSRKDLNDVLEIDLGNIDFEKLNNLLIRNIEIKENIVA
                     ADPREQNLRKALNLGHTFAHALESLSYKTDNPVLHGYAVAWGMICELYLSHIKTGFPK
                     DELMKMKYFIRENYGTYDCGCTDYSEILELMKHDKKNENREINFTLLAGVGEVKINQT
                     ATNEEIKESLDFLAY"
     gene            5019..5312
                     /locus_tag="PLN75_04885"
     CDS             5019..5312
                     /locus_tag="PLN75_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRG03486.1"
                     /translation="MNKEEIEIMENAAYMLKAISNATRLRVISLLNESDELSVSQMVE
                     ELDCEQSLLSHHLTDMRARGVLNCRKDGKNCFYSLKNKQITQILSCIQSCNSN"
     gene            5338..5559
                     /locus_tag="PLN75_04890"
     CDS             5338..5559
                     /locus_tag="PLN75_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRG03487.1"
                     /translation="MTNIIKKNILGLIGVMVGAMGGYAYYYFIGCSSGSCPLTSNPYI
                     SVIFGAIIGYLFLDMFKKTEKKEKTDETD"
     gene            5546..5803
                     /locus_tag="PLN75_04895"
     CDS             5546..5803
                     /locus_tag="PLN75_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03488.1"
                     /translation="MKLIKNLFSGWTPDRFLRLALGIIIGIIYVLDGQAVYLLFAVFL
                     LVQAFLNMGCGCATSNCATASGKDKKTEYEFEKIKTTKTDV"
     gene            5796..6014
                     /locus_tag="PLN75_04900"
     CDS             5796..6014
                     /locus_tag="PLN75_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRG03489.1"
                     /translation="MYSTFDVNGEGCLVCNNQVLGRLGKLQGVFGAEVDYINGRVVVS
                     HTDEVTREDIAGVLNELGFFETKTNCEI"
     gene            6031..6534
                     /gene="trxA"
                     /locus_tag="PLN75_04905"
     CDS             6031..6534
                     /gene="trxA"
                     /locus_tag="PLN75_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRG03490.1"
                     /translation="MKKIIFATFLSLIVAVSFAATPDKNTNQNSTKENNNKTEKNKMG
                     TIHLTKEEFLKKVVDYEKNPTEWKYLGDKPAIIDFYADWCAPCKMIAPVLEELAEEYA
                     GEIYIYKINTEIEQELAGAFGIRSIPTLLFVPMGEAPQMAQGAMPKNAFKEAIDSVLL
                     KKEKVTN"
     gene            6538..7056
                     /gene="trxA"
                     /locus_tag="PLN75_04910"
     CDS             6538..7056
                     /gene="trxA"
                     /locus_tag="PLN75_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRG03491.1"
                     /translation="MRKILYFTLAVILTAGLAACSGNKTEKDQKAKPTAETVQQVPAT
                     EPAPAKSGEPIHLTKAEFLQKVVNYEANPSSWKYVGDKPCIIDFWATWCPPCKVIAPI
                     LDDLAKEYEGQIYVYKVDVDQEKELAQAFGIQSIPTLLFVPLQGEPKAEVGAISKEVF
                     KERIDNFMLAKK"
     gene            7180..7575
                     /locus_tag="PLN75_04915"
     CDS             7180..7575
                     /locus_tag="PLN75_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRG03492.1"
                     /translation="MENMFIEVQSKFDFEQTVAVLTEEILAANWKVLVTHNLQESLHK
                     NGYEVLPVKVIELCNPHHSSKILSRDAERIYTNLMPCRIAVYDKSDGTTHVSLMNSGM
                     LAAQLGGIVDEVMSAAFADSMAFIQLVKR"
     gene            7691..8197
                     /locus_tag="PLN75_04920"
     CDS             7691..8197
                     /locus_tag="PLN75_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03493.1"
                     /translation="MKKIGILTLVALFALSFTAFSQPQRPNRNGQPASRGVNWTPEQR
                     AENLTKELNLTDIQKSSLVDFYKKQDKIREENRAEREKQRDQVMKDREKTREEYRAER
                     EKELALQDAELEKIIGKEKMDEHKKIRQQRLDRMNENRRERPDMGRRGNRDQKNDSLP
                     PVREKGKK"
     gene            complement(8309..9169)
                     /locus_tag="PLN75_04925"
     CDS             complement(8309..9169)
                     /locus_tag="PLN75_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRG03494.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIR
                     NGDRLALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERKPSAEELAE
                     ELDIPVDKIADTMKVSGRHVSVDAPFVEGEDNSLLDVMINDDSPNADRVLINESLSKE
                     IERALSTLSEREHDIVKKFFGIGVPEMTLEEIGVEFGLTRERVRQIKEKAIRRLRQSS
                     KSKLLKTYLG"
     gene            complement(9416..10237)
                     /locus_tag="PLN75_04930"
     CDS             complement(9416..10237)
                     /locus_tag="PLN75_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRG03495.1"
                     /translation="MKKHILTITLLLFFITLSISAAKVDTVVTYSPAMKKAIKALVVT
                     PDTYNDSKSFPVFYLLHGYSGNYTSWVKDMPATLALADQHQFIVVCPDGGFSSWYFDS
                     PTDSTFRYETYVTDELIRWIDRKYKTIAKREGRAISGLSMGGHGGLYLGFRHQDVYGA
                     CGSMSGGVDIRPFPNNWDISKRLGKQTQRPENWENHTVINQLHLLTPNSIKIIFDCGT
                     EDFFYNVNVKLHEELSYRNIPHDFISRPGGHDRSYWDNAVKYQAQYFADYFKTYK"
     gene            complement(10362..11888)
                     /locus_tag="PLN75_04935"
     CDS             complement(10362..11888)
                     /locus_tag="PLN75_04935"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRG03496.1"
                     /translation="MSTKTKTFIYLAALVVAVSAATSLATTYVQNKNNSNTNFIENAE
                     NPTQNARARFANAVPGIETDFTVAADLTVHAVVHVKTKAPRRQSQDFFSDPFFDFFFG
                     PQQRQQQQQQTPEEAQPLGAGSGVIISNDGYIVTNNHVIDGATEIEVTLNDKRTFKAK
                     LVGTDTSTDIALLKVEATNLPSIPFGNSDDLKVGEWVVAVGNPFNLTSTVTAGIVSAK
                     ARNIGIIGTNQYGQRTNNMSIESFIQTDAAINPGNSGGALVNTKGELVGINTAIASQT
                     GTYAGYGFAVPSSIVQKVVTDLRQHGVVQRALLGVSIGDITTEIAKEKELKTLDGALV
                     GDVVDGGAADKAGIKKGDVINEINGVKVKSSSELQEQVSRYRPGDKITVGYVRDNKQL
                     RSNASLTNAEGTTEMVKNVTAPKDLGIQLKPIDNTVKEALGIDAGLEVTAVNQGKFKE
                     QGVQQGYIILKINNQTMSSVNDFEKVYNDASKAKKALNIAGVYPTTGKIAYYKIDLSK
                     "
BASE COUNT         3317 a         2455 c         2581 g         3794 t
ORIGIN      
        1 tataaagtta gttttatata tttttttatt taatttgtat ctcaatgttt cagaatcctc
       61 tgacttttac cacaagttcc cccttcaggg ggttaggggg caggcctttt aaagcatcat
      121 cactgcctcg tttcccatgt tgaacaataa atcgatgata ctcaaatcgg gtaaaaaacc
      181 gaatttattt tcaaatacct gataataagg cttaatatga gatattgtct ctttcttggg
      241 atgaatttta tctcgaaaat cgatgttatc ttctgtcggt tccttgtatt tttctgttag
      301 tataattttc ggattttcat tcaataattg aagtatcaaa gttagcaatt caagattata
      361 atcccaaaga aaagtccatt tattttcgta gaaaggtcgg aaatcatcct ggtaatactc
      421 aaagaacggg ctgttcatgt atgccgagga gatagctctc cagtgagtct gctgccagtt
      481 gccatgtgat gaaattctca catcgcgtat gggatttttc ctgccgctgt tgctctccac
      541 cggtatgctc agtgtttcca ctccgttagc tgtggctatt ctgcaccggt ttcggtacga
      601 ttgttttacg taattttcac acgcttccag ccaaacattt tcagcattca gcaaagcagc
      661 aaagtacgaa acgggtggga gataggcagt ggaaaaccta ccccctaacc ccctaaaggg
      721 ggaatttgtt aaagtaatga ttattttttc tgcgcttgat gatatgcaat cacttgctcc
      781 cccttcaggg ggatgggggg cagattgtag tgggctattt tcttgcattc ttaaagaatc
      841 tttcgaacct gattttgccg ttgaagagcg atttgtcctt attcaacgat aaccaaacga
      901 acatcgggcg tcccaccaca tgatcttccg gaacaaagcc ccagtagcgc gagtcggatg
      961 atttgtgtcg gttgtcgccc atcatccagt aataatccat ttggaatgtg tagcttgttg
     1021 ccggtttatc gttgatgaaa aattgtccgt ttttcacttc ggttttatta ccctcgtaat
     1081 tttttatgca acgttcgtaa atggcataag tttctttgtt gagaggaatt gtactgcctt
     1141 tttttggtat ataaacaggg ccgtagttgt cgcgtgtcca tttcaggcct ccgccgagcg
     1201 gatatacatc atcgtcgttt cggtggtaaa caaaggctga atcgggctca atcaccattt
     1261 tgataactcc tcgtgtgttg agcatcattt tgtaagtttc ttcggtgagc gggaaatgat
     1321 ataccggaaa atttgtattc aatccaagtg ttttcagcag ttcaaagcgc tcggcattgt
     1381 caatttccca gaagtcttcg ttgctgatgt tcattttatc aagtgcttcg cgggtaagtc
     1441 gggtgccgtc tgtctgtaca aaatagttga attgtatccc gggaaatttt ggttgaggag
     1501 ttccgttcac cactatttga ttgttgatta tttgcaacgt gtcaccgggt attcctacgc
     1561 atcgttttac gtagttttcg cgcctgtcaa ccggtcggta catgatttcg ccatactgag
     1621 cggctttgga tttcacaatt tcgcgtccgt accggtagca gagttcggcc gtaggctcca
     1681 cctgcagata gttaatcaca tacgccagcc cttcgcgata gcaaagtgtg taataatcag
     1741 ggttttgtgc tttcacggca atggtgtcgc cggcaggaaa attaaaaact acgatgtcgt
     1801 ttctttcaat ggaatcatgc cctttcagtc ttcggtattt cacctgcggt ttttccaggt
     1861 acgatttcga attcagaatt ggaaatgtat tctgtaccag tgggaatgaa aacggtgtca
     1921 ttggtacccg tggcccgtag ctggctttac ttacagccag aaaatcgcct accagcaggg
     1981 ttttctccag tgaagaagtg ggaatctgat aattctggaa cagatagtta ttgatcagat
     2041 atacagctat caacgcaaat ccgattgcat ctacccagct cattaccgat ctcacggtct
     2101 tgcttttgga atttttccac cacgtccatg ggatgaattt agtgataaac gcatcaaaaa
     2161 tgagcggcag caccagcatc caccagtaat tgcccagcca gataacgaaa agtatgtaaa
     2221 caatgcacca aatgatagct ttaatccatt gactggttgg cttgtcgaaa cgtgatgccg
     2281 gttgagggtt agtttgagtt gtcatgtgtt atttgaaatt tttgcttaga aaaaacaaaa
     2341 tttattatca aaaaaatagt tattatgttg aagtcatctt gacttttaaa acatagagaa
     2401 caatagtctt attttgtcag tttttatatt tattttaatg ttcttcgaaa acaatagttt
     2461 aaaaatatcg aacctgcctg cggcaggcag ggatattttt tctgtgttac cgaaaatatg
     2521 agaataaatg taaattattc tatgttttct attgttttaa aaaaagtcga gacgagttcg
     2581 taaaataaat atagatttca ttcagaactt cagtaaatct cccatcccca gaaatccttt
     2641 ttttcctttg gtaaattccg ctgccactac tgctccgagt gcaaatcctt cgcggctttt
     2701 ggcgtcgtgg gtgatttcaa tgctgtcaac agcactttcg taacgaatag tatgaatgcc
     2761 gggcacttca ccatcgcgaa tagattttat ggccagctcg tcagaattat tttcagtgtc
     2821 ttttacccac accatttttc tgtccagatt tcccaaaatt ccttctgcca gcgtaatggc
     2881 tgtgcccgac ggagcatcga gtttttgagt atggtgtatt tcagtcattt ctacgttgta
     2941 atccgggaat tggttcatca ttttagccag atagctgttc agtgtgatga aaatattcac
     3001 accgaggctg aaattcgatg accagaatag cgtatgattg tttttttcga cattattctt
     3061 tacctcctcc aaatgctctg tccagcctgt agtgcccgat actacggcaa ctccggcccg
     3121 gaaagcttcg tcgtaatttt gcagtgcaat gtgcggggtg gtaaattcaa tggctacatc
     3181 agcgctcctg aaggcttccg actgaaagtc ctcccgattg tcgatgtcga taactgacac
     3241 aatttcatga ccacgttcaa gggcaattct ttcgatgatt ttgcccattt ttccatatcc
     3301 gattaatgct attttcataa gtccctctga ttctccacaa gaaggagatt tttgtatgta
     3361 gtatgaatat tttactaaac ttgttggttg ctctaaaaaa accttccctt tgggaaggtt
     3421 tggatgggct aatactgccc cgttcccagc agcaccagcg tgcaggctac ttctgcttct
     3481 atattattct ttttcagaac cttataaaat tcttcatttt ctgttccggg attaaaaatg
     3541 acccttcgtg gatttaatga aacgatgtag tcgtagtatt ccggctgacg ctgtgcaccc
     3601 acataaagtg tcacagtgtt aatatcagca taatttttcc tttcggtgtc tataattcgt
     3661 ccgaaaatct tcctggggcg cagaccgagt agttctatct cgtgcccgtg tgccaaaagt
     3721 ctttctgcag ctttgtaaga atagcgatca ggattgtctg atgcgccaat gattaatgtt
     3781 ttcttcatat cagtaatgtt aatatgccag aaaatccagg ctttccttta tctcttcgtt
     3841 ggtggctgtc tgattgattt ttacttcacc cacacctgcc agaagcgtga agttgatttc
     3901 tctgttttcg ttttttttgt cgtgtttcat cagttccagt atttcgctgt agtcggtaca
     3961 tccgcagtcg tatgttccgt aattttctct gatgaaatac ttcattttca tgagttcgtc
     4021 tttcgggaat ccagttttaa tgtgcgaaag atacaattca caaatcatac cccaggcaac
     4081 tgcataaccg tgaagtaccg gattgtctgt tttgtagctc aggctctcca gagcgtgagc
     4141 aaaggtatgt cccagattga gtgctttacg caggttttgt tccctggggt cggcagcaac
     4201 tatgttttcc ttaatctcga tatttcggat cagcaggttg ttgagtttct caaagtcaat
     4261 gtttcccaaa tcgatttcca atacatcgtt caggtctttt ctgctgctga taagggcatg
     4321 ttttatcatc tcggcatatc cggaacgtag atttttatca tcaagtgttc tgaaaaattc
     4381 cacattgata agtacctttt ttgcaggata aaaagccccg atttcatttt tcaatcccag
     4441 aaaatttata cctgttttac caccggtagc cgcatctact gctccaagaa gtgttgtgga
     4501 tacattgatg taatcaatac cccgcttgaa tgtggctgcc gcaaatcctc ccatatcggt
     4561 tatcatgcct cctcctacgt tgatcatcag cgatttacgg gttgctccat tttcggttaa
     4621 ataccgccag atttgctgca gcgaatcaat gtttttctgt gcgtcgcctg cctgaacagt
     4681 tatctgatgg cctttcctta ttttttcgga ttcgaaaagg agtggcaggc atttttcgcg
     4741 ggtattggtg tcggtgagaa tataaatatc atcactctcc caatcgcaaa tttcgtactc
     4801 cagctggcgg attatatgtg tacttattat agtattcggg gccggcattt attgtttttt
     4861 aaattgtaca aagatacgct ttattcttaa aattagcttt ttattttatc gttttttagt
     4921 ttagacacgt gtttcaaagt tgtttagtta aaaataataa aattcttatt tatatatgaa
     4981 catatattca tacatttgca ttgtaattcg aagataatat gaacaaagaa gaaatagaaa
     5041 taatggaaaa tgcagcgtat atgttgaaag ccatatcaaa cgctacccgt ttgagggtaa
     5101 tttctttgct gaacgagtcg gatgaactgt ctgtttctca gatggtagag gagcttgatt
     5161 gtgaacagtc actgctctcg caccacctca ccgacatgcg tgcgagaggt gttttgaatt
     5221 gccgtaagga tggaaaaaat tgcttttact cattgaagaa taagcaaatt acacaaattc
     5281 tttcctgtat tcaaagttgt aactctaact agaaatttac ataaaaacag aaatgatatg
     5341 acaaacataa ttaagaaaaa tattttagga cttatcgggg tgatggttgg cgctatggga
     5401 ggatacgctt attattattt cataggctgc agctcaggta gttgcccgct aacttccaat
     5461 ccttacatca gtgtcatttt tggagcaata atcggatatt tattcctgga tatgttcaaa
     5521 aaaacagaaa aaaaggaaaa aacagatgaa actgattaaa aatctttttt ccggctggac
     5581 acccgaccgt tttctgcgcc tggcgctggg aataataatt ggaattatat atgtattaga
     5641 tggtcaggct gtttacttgc tttttgccgt ttttttactg gtgcaggcat ttctgaatat
     5701 gggatgcgga tgtgcaactt ccaactgtgc aactgcctcc ggaaaagaca aaaagacaga
     5761 atacgaattt gaaaaaataa aaacgacaaa aactgatgta tagtacattt gacgtaaacg
     5821 gagaaggatg cctggtgtgc aacaatcagg tactcggcag gctgggtaaa cttcagggtg
     5881 tatttggcgc cgaagtagat tatattaacg gtagagtagt ggtgtcgcat accgacgaag
     5941 tgacccgcga agatattgcc ggcgtactga acgaactcgg atttttcgaa acgaaaacaa
     6001 actgtgaaat ttaacaatac aaatacgaca atgaaaaaaa taatttttgc aactttttta
     6061 tccctcattg tagctgtcag ttttgctgcc acaccggata aaaataccaa tcagaattca
     6121 acaaaagaaa ataataacaa aacagaaaaa aacaaaatgg gaacaattca tttaaccaaa
     6181 gaagaatttc taaaaaaagt agtggattat gaaaaaaatc cgaccgagtg gaagtattta
     6241 ggcgacaagc ctgctataat tgatttctat gccgactggt gtgcaccatg taaaatgata
     6301 gcacccgtgc tggaagaact tgcggaagaa tacgccggtg aaatttacat ttataaaata
     6361 aacactgaaa tagagcagga actggcaggc gcatttggta tccgcagtat tcccacgctg
     6421 cttttcgttc cgatgggcga agcaccgcag atggctcagg gtgcaatgcc taaaaatgca
     6481 ttcaaggaag caattgacag tgtgcttttg aaaaaggaaa aagtaacaaa ctaaggtatg
     6541 agaaaaatat tgtatttcac acttgcggtg atactcacag cggggctcgc agcctgcagc
     6601 ggcaataaaa cggagaaaga ccagaaggcc aaaccgacgg ctgaaacagt tcaacaagtg
     6661 cctgcaaccg agccggctcc tgccaaatca ggtgaaccca ttcatcttac caaagctgaa
     6721 tttcttcaga aagtggtgaa ctacgaggct aatccgagtt cgtggaagta tgtgggcgat
     6781 aaaccctgta tcatcgactt ctgggctacc tggtgtccgc catgtaaagt aattgctccg
     6841 attctggacg acctggccaa agaatacgaa ggtcagatat acgtctacaa agtggatgtg
     6901 gatcaggaaa aagaacttgc ccaggcattt ggtattcaga gtattcccac tttgcttttt
     6961 gtgccgctcc agggtgaacc caaagctgaa gtaggagcca tttccaaaga ggtattcaaa
     7021 gaaagaattg ataattttat gcttgctaaa aaataagttt gatttttgat tgattgattg
     7081 gaaaatccgg ttgtctcatt tatcatcctg ctggttgaat ctgagttgcc ggattttttc
     7141 ctgaaaaaaa atgctaatcc aaaaactaaa ataataaaga tggaaaatat gtttatcgaa
     7201 gttcagagta aatttgattt tgaacaaaca gtagctgtat tgactgaaga aatcttagct
     7261 gccaactgga aagtactcgt aactcacaat ctgcaggagt cgcttcacaa aaacggatac
     7321 gaagttttgc ccgtcaaagt gattgaactg tgtaatccgc atcactcatc gaaaatcctc
     7381 agcagagatg ccgaaagaat ttatactaat ctgatgccct gcagaatagc cgtttacgat
     7441 aaatcagacg gtacgactca tgtgtcgctg atgaactcgg gaatgctcgc agcacaactc
     7501 ggaggaatag tagatgaagt aatgtcagct gctttcgctg attccatggc ttttatccag
     7561 cttgttaaac gataatttgg tgatacttaa gttaaaaaaa ataaataatt aaaggcatat
     7621 taaacgttgg gaagttctgt aagtcaaaag gataaacaat ttactttata aacaattaat
     7681 atttacgaaa atgaaaaaga ttggaatttt gactttagtt gcactttttg ctttgagctt
     7741 tacggctttt tcacagcctc aacggcccaa ccgtaacgga cagcctgcct cccgcggagt
     7801 aaactggact cccgagcagc gcgctgagaa tttgaccaaa gagctgaatc tgaccgatat
     7861 tcagaaaagc agcctggtgg atttttacaa gaaacaggat aagatacgcg aagaaaaccg
     7921 tgccgaacgc gaaaaacagc gcgaccaggt gatgaaagac agagaaaaaa ccagggaaga
     7981 gtaccgcgcc gaacgtgaaa aagaactggc tcttcaggat gcagaactgg aaaaaattat
     8041 cggaaaagaa aagatggatg aacacaaaaa aatccgtcag cagagacttg acagaatgaa
     8101 cgaaaaccgc agagagcgcc ctgatatggg ccgcagaggc aaccgcgatc agaaaaacga
     8161 ctctttgcct ccggtacgtg agaaagggaa aaagtaaaac cggcggtgtc ggactgacga
     8221 atcaatgatt tataatttaa tcagagaaaa aagagaagca actcaaattt tgagttgctt
     8281 ctcttttttc atttatttat attcagtctt aacctaaata cgttttcagc agtttgcttt
     8341 tggaactttg tctcaaccgc ctgattgctt tttcctttat ttgtcgtacg cgttcgcgtg
     8401 tgagtccgaa ttccacgcct atttcttcga gtgtcatttc aggaacaccg attccaaaga
     8461 attttttcac aatatcgtgt tcacgctcgc tcagggtaga tagagctcgc tctatttctt
     8521 tggagagcga ttcgttgata agcacccggt cggcattggg agaatcatcg tttatcatca
     8581 catcgagcag gctgttgtct tcgccttcca cgaaaggagc atccaccgat acatgtctgc
     8641 ccgaaacctt catggtgtcg gcaattttat ctaccggaat gtcgagttcc tcagccagct
     8701 cttcggccga aggtttccgt tcgttttcct gctcaaattt ggagaaagct ttattgattt
     8761 tattcaacga gcccacctga ttcagcggta agcgtacaat acgcgattgc tcggccaaag
     8821 cctgcagtat agactgacgt atccaccaca cggcgtagga aatgaactta aatccacgtg
     8881 tttcatcgaa tttttcggcc gctttaatca gtcctaaatt gccttcatta atcaaatccg
     8941 gaagacttaa gccctgattt tgatactgct ttgctactga aaccacaaaa cgaagattgg
     9001 cacgggtaag tttttccaaa gccaggcggt caccgtttct gatgcgctga gcaagttcca
     9061 cttcctcctc aacagttata aggtcttcac gtccaatctc ttgcaagtat ttgtcaagtg
     9121 atgcactctc acgattggtg attgattttg taatctttag ctgtctcata ttggatttga
     9181 aaattaagcc tgcaaaagta ctgttttttt tgtgattttg aaaatttatt gtttaaaaag
     9241 aatggtaaaa atatttgaaa atgaataatt tcccgaatat tattgccata aattgactga
     9301 aataacagat tttattgtgt caataatgta ttaacacctt gagttgaatt attgttttga
     9361 gattgctttc aaaaaaacac attatttgac atcaaaaaag ttgctcaaag agatattact
     9421 tgtatgtttt gaaataatca gcaaaatatt gtgcctgata tttcacggca ttatcccagt
     9481 atgagcggtc gtggcctccg ggacgtgata tgaagtcgtg cgggatgttt cggtagctga
     9541 gttcttcgtg aagttttaca ttcacgttgt agaaaaaatc ttcggttccg cagtcgaata
     9601 ttattttgat gctgttcggc gttaagaggt gcagctgatt tatcaccgtg tgattttccc
     9661 agttttcagg cctttgagtt tgtttaccga ggcgtttgct tatgtcccag ttgttgggga
     9721 acgggcgaat gtccacgccg ccactcatgc tcccgcaggc accgtacaca tcctgatgcc
     9781 tgaatccgag gtacaatcca ccgtgtccgc ccatgctcag tcccgaaatg gctcttcctt
     9841 cgcgtttggc tatcgttttg tatttccggt ctatccaccg gatgagttca tcggtgacat
     9901 acgtttcgta gcggaaggtg ctgtctgtcg gactgtcgaa ataccagctg gagaatcctc
     9961 catcggggca aaccacgata aattgatgct gatctgccag tgcaagcgtt gcaggcatat
    10021 ctttcaccca gcttgtgtag ttgccgctgt aaccatgcag caggtaaaaa accggaaatg
    10081 attttgaatc gttgtacgtg tcgggtgtaa ccaccagcgc tttaatggct tttttcatcg
    10141 ccgggctgta ggtgactacg gtatcaactt ttgcggcaga gatgctcaaa gtgataaaaa
    10201 acagcagaag tgtgattgtc aggatgtgtt tcttcataca atgaaatttt aaaatttttc
    10261 aaaagacttc aaaggtatct ttttttttga aaaaataaaa aaaacaggcc tttttcaatc
    10321 tgaaacagtc ctgttttttt ttgtggaaag gatgaataat attatttact cagatcaatt
    10381 ttgtagtatg ctattttacc ggtggttggg taaactcctg caatattgag tgcttttttg
    10441 gcttttgaag catcgttgta cactttctcg aaatcgttta cgcttgacat ggtctggttg
    10501 ttgattttca gaatgatgta gccctgctga acgccctgtt ctttgaattt tccctgattt
    10561 acggctgtta cttccagtcc ggcgtcaatt ccaagggctt cttttacggt attgtcaatc
    10621 ggtttgagtt gtatgcccaa atctttgggt gctgttacat tcttaaccat ttcggtagtt
    10681 ccttcggcgt ttgtcaggct tgcgttgctc cgaagctgtt tgttgtctct gacataacca
    10741 acggtgattt tatcgcccgg ccgatagcgg cttacctgtt cctggagttc agaagatgat
    10801 ttgactttta ctccgtttat ttcgttgatg acgtcgcctt ttttgattcc ggccttatcg
    10861 gctgctccac catccactac atcgcctacg agtgcaccat ccagtgtttt aagttccttt
    10921 tctttggcaa tttcagtggt gatatcgccg atgctaactc caagcagtgc ccgttgtacc
    10981 acgccgtgct gacgaaggtc ggtaaccact ttctgaacaa tgctcgaagg aactgcaaaa
    11041 ccatatcctg catacgtacc ggtttgcgat gcgatggcag tatttatccc aacgagttca
    11101 cctttggtgt tgaccagagc accaccactg tttccgggat tgatggccgc atctgtctgt
    11161 ataaatgatt cgatggacat gttgttggtg cgttgtccgt attggttggt accgatgatt
    11221 ccgatatttc tggctttggc acttacaatt ccggcggtga cagtggaggt aagattaaag
    11281 ggattgccca ctgccactac ccattcgccc accttgaggt catctgaatt tccgaaagga
    11341 atgctcggaa ggttagtagc ttcaactttg agcaatgcga tgtcggtaga agtatctgta
    11401 cccactaatt ttgctttgaa ggtacgtttg tcgttcagtg ttacctctat ttctgttgca
    11461 ccgtcaatta catggttgtt tgtcacaata tatccatcgt tgctgatgat tacacccgag
    11521 cctgctccca gtggctgagc ttcttccgga gtttgctgtt gttgctgctg tctttgctgg
    11581 ggtccgaaga agaagtcgaa aaacggatcg ctgaagaaat cctgcgactg acggcgcgga
    11641 gcttttgttt tgacgtgtac cacggcatgc accgtgagat ctgccgctac agtgaaatca
    11701 gtttcgatac ccggaacggc atttgcaaat cttgccctgg cattttgtgt cggattttcg
    11761 gcattttcta tgaaattagt attgctgttg ttcttgtttt gaacgtaagt ggttgccaga
    11821 cttgtagcag cacttactgc taccaccaac gctgctagat aaatgaacgt tttcgttttt
    11881 gtactcataa ttattatttt tagattttta ttgtctgata caaatttagc aaatggtatg
    11941 cctaaaatgg atgaaccagc tgcttatttg gtcaaattta acattgttaa aatggggttt
    12001 aacgcgcttt aacggtgata tgaagctatg tttaaaatat ctgaatgatt cagactaaaa
    12061 aaatatctgc cgaaaaatat ttatgtccgg ttttcaggtg aaattttgtc agattgctga
    12121 cagaattaca tttaattttt tgtcgtt
//
LOCUS       DAORKP010000062        12036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_537_length_12036_cov_10.179618, whole genome shotgun sequence.
ACCESSION   DAORKP010000062 DAORKP010000000
VERSION     DAORKP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_12036_cov_10.179618"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..3020)
                     /locus_tag="PLN75_04940"
     CDS             complement(159..3020)
                     /locus_tag="PLN75_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRG03497.1"
                     /translation="MRKPILLLLTFAVAIFSGCSNSNFTKKSDGVEISIQFKVPDSAQ
                     KIRLQVINEKIIRVSATPEKNFSTEKSLMIVPQDKKADFTVEEKDNLIALHTDSLQVT
                     VDKTTGEVVFMNKNGEIILQENAGGGKSFSPIEVEGTKGYTVRQVFESPDDEAFYGLG
                     QHQSDEFNYKGKNESLFQYNTKVSVPFVVSNKNYGILWDNYSLSRFGDSRDYTDLDQF
                     KLFNSEGNEGGLTASYIPLDPSKEKIVRTEPILNYEFIPQLKNFPKDFELNNSKVTWE
                     GEIESNESGNYHFYLHYAGYVTVYLNNEVVVKERWRTAWNPNDYKFAYNMEAGKRIPI
                     RVEWEPDGGISYVALKALSPLPEAEQNKLSIWSEMGNEIDYYFVYGKNMDEVISGYRT
                     LTGKSQIMPKWAMGFWQSRERYKTQYELVNTLKEFRERKIGIDNIVQDWFYWPEKEWG
                     SHKFDPERFPNPKEMVDTVHQLNAKIMISVWPKFYTTTDHYKQFDTKGWMYQQAVKDS
                     IRDWVGPGYIASFYDAYSEGARKLFWKQMEENLYPKGFDAWWMDASEPNVQDNTEIEY
                     RKKLAGPTALGPSTKYFNAYALVNAQSIYEGQRGVDPNKRVFLLTRSGFAGTQRYSTA
                     TWSGDIGTRWEDMKAQITAGLNFAMSGIPYWTMDIGGFCVEKRYENGQRLFDSMGIEN
                     ENLKEWRELNARWYQFGAFSPLYRAHGQFPYREPFNIAPENHPAYQTINYYNKLRYRL
                     MPYIYSLAGMTYHNDYTIMRALVMDYGKDIKVNNISDQYMFGKSLMVCPVYEYKAIKR
                     NVYFPNTTGWYDFYTGKFIAGGTQQSVEAPYTRMPLYVPEGAILPFGPEIQFTDEKKP
                     EYITLFVYGGKNGSFTLYEDEGVNYNYEKGAFAMIDFNYDEGSKTLEIGDRKGSFEGM
                     LQNRKFNVVFVNQSSPKAYDPDKKGVEVNYRGIKQNIRL"
     gene            complement(3039..3569)
                     /locus_tag="PLN75_04945"
     CDS             complement(3039..3569)
                     /locus_tag="PLN75_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRG03498.1"
                     /translation="MEAEIYIQLFLSFFKIGLFGFGGGYAIISLIQHEIEVHGWMTQS
                     EFTDIIAISQMTPGPIGINSATYVGYTASGTVLGSIIATFAIVLPSFLIMLTLVRFFF
                     ALQGNKYLEWAFLGLRPAVIGLIGAAAILLMNRDNFIDYKSFIIFLLAFLLSFTGKFH
                     PILLILMAGVAGFVIY"
     gene            complement(3569..4105)
                     /locus_tag="PLN75_04950"
     CDS             complement(3569..4105)
                     /locus_tag="PLN75_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRG03499.1"
                     /translation="MFMTFVRIGSFTIGGGYAMIPLIQKEVVENKKWMDAREFIDMLA
                     MAQSAPGVIAINTAIFIGYKMKGLKGSLVTTLGSALPSFIIILAVAMVFTNFRDNVVV
                     EKIFRGIRPAVVALIAAPLYKMARSAGVTWKTVFIPILAVLLIWLAGVSPIWIVLAAI
                     AGGIIYGALHARNLSKSI"
     gene            complement(4286..5215)
                     /gene="queG"
                     /locus_tag="PLN75_04955"
     CDS             complement(4286..5215)
                     /gene="queG"
                     /locus_tag="PLN75_04955"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRG03500.1"
                     /translation="MTEFIKNTALEIGFDACGIAKAGFLEEDALFLKSWLGDEFNGEM
                     SYLERNFEKRTDPTKLVEGCKSVVVTLMNYFPASAQQAAWPKISKYAFNRIDYHTVIR
                     EKLLLLENRIKEKWGKDCFNPSQQHRFVDSAPILERRWAAQAGLGWIGKNKMLINESL
                     GSFCFIGILLINKELAYDEPVKNRCGTCRKCLDSCPTGALTENEGLDARKCISYQTIE
                     KKGEITQEIRSKLSGYAYGCDICNDVCPWNKSRAKPNRHQEFQPLQEVINWNSDDWKN
                     LTQEEFNRIFKYSAIQRAGYDKLKQNIDFSGVE"
     gene            complement(5486..6613)
                     /locus_tag="PLN75_04960"
     CDS             complement(5486..6613)
                     /locus_tag="PLN75_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRG03501.1"
                     /translation="MLKKSIFFVLLVLLTACNNPGKFKVSGNITDAEGKVLYFEKTGL
                     LKDSLIDSVKLSKEGTFKFRTAVPVYPELYRLRLDNQQLVLGIDSTESIEIQGSASDL
                     ISSAITGSVQTADIQALRKSVIELQKQFDAYPAVKDVEKSKSLADSLQMRVEEHKKFA
                     QGIILKNPGSMAAYFALYQQISGNYIFSPYVKAERPYYQAVATSFHSIMPEYERSKSL
                     YNLVLSAIREDRMARRQNIPGSIGEIQKTGFLEIELKDKNGYPQKLSSLIGKPILLDF
                     SAYGTETSIDYTFALREIFTKYSPKGLQIYQVSVDQSKMLWQRSVSNIPWTCVHDETG
                     IAAKSYNVQAIPTMYLISKEGNIIGRYRSIKSMEADLGKVL"
     gene            complement(6619..7167)
                     /locus_tag="PLN75_04965"
     CDS             complement(6619..7167)
                     /locus_tag="PLN75_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRG03502.1"
                     /translation="MKKVFIFLADGFEEIEAIAPIDILRRAELDVVTVSISDSKTVTG
                     AHGIKVEADQLFTETTFGENDYYVLPGGYDGMLNLSAHQGVNELLKKQHSEGKKLAAI
                     CASPSVLGKLGILEGKEAICYPGFEGKLTGAVISKKSVVEDGNVITGKGPGVAVQFAL
                     KIVESLKGKETASQVSDSLMLN"
     gene            complement(7204..7950)
                     /locus_tag="PLN75_04970"
     CDS             complement(7204..7950)
                     /locus_tag="PLN75_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRG03503.1"
                     /translation="MLGSALFCLLTFLILWFVFMPAVVRPTEDEGIMVSFGDNIDGMG
                     FGEMIAGVEEMSSPPIPKQVSEQQELITQETEKSVVVPKQQTKPKTKPVTITEQELQQ
                     QELQRREAAAAAEAKRRQEAAARSENLVGSAFGSGGGTGSGTTEGDTRQGNPAGSGTS
                     GGHGWSLSGRSLIGTLATPSYPNNVEGKITVSIRVDASGRVTSATVGSPTTISDAQTR
                     SAALSAARTTRFSSGSGVSVGTITYNFKLK"
     gene            complement(7978..8424)
                     /locus_tag="PLN75_04975"
     CDS             complement(7978..8424)
                     /locus_tag="PLN75_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRG03504.1"
                     /translation="MALKRRSRLESGFPMSSMTDIIFLLLLFFVISSTMTTPNDIKIN
                     LPQSGAKTATEQVVARVSIDKEGKYYIAGNKEKAVEVSPEMLEEQIMNLISRDTATFL
                     ALYADQEIAYKEVVRVLDIANKNKLKLVIATSASTGESDSKNVDNQ"
     gene            complement(8497..9192)
                     /locus_tag="PLN75_04980"
     CDS             complement(8497..9192)
                     /locus_tag="PLN75_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRG03505.1"
                     /translation="MNLQILLQQTISQAVTAEAQAVPEKMNLWQMTVYGGPIMIILGI
                     MLALAVYIFIERLITINKASKEDKSFMNRIKDYILDGKIDSAQKLCRDTDSPASRMIE
                     KGISRLGRPMNDVLLAVENVGNLEISKLEKSLVILATISGGAPMLGFLGTVTGMVLVF
                     YNMTQNSTGVVQLIDLSSGMYQAMVTTVGGLIVGILAYFAYNYLISRVDSVVRNLESK
                     TLEFMDLLNEPSK"
     gene            complement(9248..9967)
                     /locus_tag="PLN75_04985"
     CDS             complement(9248..9967)
                     /locus_tag="PLN75_04985"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRG03506.1"
                     /translation="MTKLSVNINKIATLRNSRGGDMPNVVKVALDCERFGAEGITVHP
                     RPDERHIRYADVYDLKPVLTTEFNIEGNPIPEFIKLVKEIKPAQVTLVPDAHDALTSN
                     AGWDTRKNLIFLREVISEFQKEGIRVSVFVDTDLANIEYAAKTGTDRIELYTEPYAAA
                     YKNDKFAAVQPFVEAAREAHRLGLGINAGHDLNLENLAFLHQNIPWLDEVSIGHALIA
                     DALYLGLEETIRQYKARLTEI"
     gene            10289..11179
                     /locus_tag="PLN75_04990"
     CDS             10289..11179
                     /locus_tag="PLN75_04990"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRG03507.1"
                     /translation="MESKETKRIALFGNLHRTEMENELLELVQFFHQMNVVVLLDEYL
                     QLYLDEKFHKSFDRVELITDDNFSADLALSIGGDGTFLNTAARVGKKDIPILGINTGR
                     LGFLADVAKDEIIPALQAIFKHEYTIEKRSVLKLQTLDGSRLDSPYALNDIAISKQDS
                     SSMIIVNAYANGELINIYQADGLIISTPTGSTAYSMSAGGPIVVPQARSFVISPVASH
                     SLNVRPLIVPDDWEIDLQVISRSNSYLVSIDGRSKILDHSTRLRLKKADYSIQVLKPL
                     NHTFFETLRNKLLWGADVRN"
     gene            complement(11194..11823)
                     /locus_tag="PLN75_04995"
     CDS             complement(11194..11823)
                     /locus_tag="PLN75_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03508.1"
                     /translation="MNQIKSILFSLLVVALFSCKPAEPPAPYQYNTAPAFTWGLADFY
                     GNYYTNYGVQNNVLTLHLFTEKLVINKDNELDGTGQYLIIQDIFSAPADTLLAAGTYR
                     MAETGEPFTFFAGKKYEDNREPIPSGAYMYYIEADPTKSKVTYVTDGTMNVTVTESGR
                     YDIQCNFTLDGKTELKGTFKGEIPHFDRVATAPAGISRRNLIPVIEVKD"
BASE COUNT         3257 a         2632 c         2430 g         3717 t
ORIGIN      
        1 gctactatca attccggctg aactacttct tcgggtttac aggatgaagt gcagaataaa
       61 agtaaagcag aaacgagaaa gatgtgaaaa aatcttttta tcataaaatt cagaaaagat
      121 aaatttgagg ccaatattat actgtatttc atgcctgact acagcctgat gttttgttta
      181 attcccctgt aattgacttc tactcctttt ttatcaggat cgtaggcctt tggtgaagat
      241 tggtttacaa acaccacatt gaattttctg ttttgcagca ttccttcaaa agaacctttt
      301 ctgtcgccga tttccaacgt tttagaaccc tcatcgtagt tgaaatcaat cattgcgaaa
      361 gcaccttttt cgtaattgta attcacgcct tcatcttcgt acaaggtaaa tgagccgttt
      421 tttccaccat aaacaaaaag ggtgatatat tcaggttttt tctcgtcagt gaattgaatc
      481 tccgggccaa aaggcaggat agctccttca ggaacgtaca aaggcatgcg ggtataaggt
      541 gcttccaccg attgctgtgt gcctccggca atgaatttcc cggtatagaa atcgtaccag
      601 cccgttgtgt tcgggaaata aacgttgcgt ttaattgctt tatattcata aaccggacat
      661 accatcagcg attttccaaa catgtactga tcactgatat tatttacctt gatatctttt
      721 ccgtaatcca tcaccagggc acgcatgatg gtgtagtcgt tgtggtaagt cattccggcc
      781 agcgaataaa tgtacggcat cagtcggtag cggagtttat tataataatt aatggtctga
      841 taagcaggat gattttcggg agcaatatta aacggttcgc gatagggaaa ttgtccgtga
      901 gccctgtaga gaggcgaaaa tgcaccaaat tggtaccagc gggcattcag ttcacgccat
      961 tctttcaggt tttcgttttc aattcccatc gaatcgaaaa gtcgttgacc gttttcgtat
     1021 cttttttcca cacagaaacc gccaatatcc atggtccagt aaggaattcc ggacatagca
     1081 aagttcaatc cggcagtaat ctgggctttc atatcttccc atctcgtgcc aatatcacca
     1141 ctccaggtgg cggtagagta acgctgagtg cctgcaaaac ccgagcgggt aagcaggaaa
     1201 acccgtttgt ttggatcaac tccgcgctgt ccttcgtaaa tagattgtgc atttactaaa
     1261 gcgtacgcgt taaaatattt agttgaaggt ccgagtgcag ttggaccggc cagttttttc
     1321 cggtattcaa tctctgtgtt gtcctgcaca ttaggttcag aggcatccat ccaccaggcg
     1381 tcaaaacctt ttggatagag gttttcttcc atttgtttcc agaaaagctt gcgtgcacct
     1441 tctgaataag catcgtagaa agaagcgatg tagccgggac caacccagtc gcggatgctg
     1501 tctttaacgg cctgctgata catccatcct tttgtgtcaa actgcttgta gtggtcggta
     1561 gtggtgtaga atttcggcca aacggaaatc atgatttttg cattcaactg atgtacggtg
     1621 tccaccattt cttttggatt tggaaatcgc tccggatcga atttgtgact tccccattct
     1681 ttttcaggcc agtagaacca gtcctgtaca atattatcaa tacctatttt tcgttcgcgg
     1741 aattctttca acgtattaac caattcatac tgagttttgt atcgctcgcg gctttgccag
     1801 aatcccatgg cccatttcgg catgatttgc gattttccgg tcaaagttct gtatccgctt
     1861 atcacttcgt ccatgttttt gccgtaaacg aaataataat caatttcatt acccatttcg
     1921 ctccaaatcg aaagcttgtt ctgttctgct tcaggaagtg ggctcagcgc tttcaaagct
     1981 acgtaggaaa tgcctccgtc gggttcccat tccacgcgga tcggaatccg tttaccggct
     2041 tccatgttgt aggcaaattt ataatcgttt ggattccagg cagttctcca tctttctttc
     2101 actacaacct cgttgttcag ataaacggtc acatagccgg cgtagtggag gtaaaaatgg
     2161 tagtttcctg actcattgga ttcgatttcg ccctcccagg tcacttttga attgttcaat
     2221 tcaaaatctt ttgggaagtt tttcagttga ggaatgaact cgtaattcag gatgggttcg
     2281 gtgcgtacta tcttttcttt tgacggatct aatggaatat aggaagcagt cagaccgcct
     2341 tcgttccctt cggaattgaa aagtttgaac tggtcaagat cggtataatc acggctgtcg
     2401 ccaaaacgag ataaggagta attgtcccac aaaattccgt aatttttatt cgaaactacg
     2461 aatggaacgg aaactttggt gttgtactga aacagtgatt cgtttttgcc tttgtagttg
     2521 aattcgtcag actgatgctg tccaagtccg taaaacgcct catcatcagg cgattcaaaa
     2581 acctggcgga cggtgtaacc ttttgtccct tccacttcaa tgggtgagaa agattttcct
     2641 ccgccggcat tttcctgaag gataatttcg ccgtttttat tcatgaaaac gacttctccg
     2701 gtcgttttat ccactgtaac ctgtagagaa tcagtgtgaa gcgctatgag attatctttt
     2761 tcctcaacag tgaaatcagc ttttttgtcc tgaggaacaa tcatcaggct tttttctgtg
     2821 gaaaaattct tttcaggtgt agctgatacc cggatgattt tttcgtttat tacctgcaaa
     2881 cgaatctttt gagcactgtc cggaacttta aattgaatag aaatctctac tccatctgat
     2941 ttttttgtaa agttagaatt actgcaacca gaaaagattg ccactgcgaa tgttaacaac
     3001 agtaaaattg gttttctcat gatatttatg tctgaaattc agtaaattac aaatccggct
     3061 acaccagcca tcagaatcag cagaatggga tgaaatttgc cggtgaatga aagtagaaat
     3121 gccaataaaa aaattataaa actcttataa tcaatgaaat tatccctgtt catcaacagg
     3181 atagcagccg ctccaataag tccgattact gccggacgaa gaccgagaaa agcccattcc
     3241 aggtatttat ttccctgaag ggcaaagaaa aaccgaacca atgtaagcat gatgaggaaa
     3301 gacggtaaaa caatggcaaa agtagctata atcgatccca aaactgtgcc cgaagcagtg
     3361 tagcccacat atgtggcact gttgatgccg atgggtccgg gtgtcatctg cgaaatagcg
     3421 atgatatccg taaattcact ttgagtcatc catccatgca cttctatttc gtgttgaatg
     3481 agcgaaataa tggcataacc acctccaaaa ccgaacaaac cgattttgaa aaatgagaga
     3541 aaaagttgta tgtatatttc ggcttccatc agatcgattt tgaaagattg cgtgcatgaa
     3601 gcgctccata aataattcct ccggcaatag ctgccagaac tatccagata ggcgatacac
     3661 cggcaagcca aattagtaat actgcaagaa tgggtataaa aacagtcttc caggtaacgc
     3721 ctgccgaacg ggccatttta tagagcggag ctgctatcag agccaccaca gccggacgaa
     3781 ttcccctgaa aattttttca accactacat tatccctgaa attggtaaaa accatagcta
     3841 cagccagtat gatgataaaa gagggtaaag cacttccgag cgttgttaca aggcttcctt
     3901 tcaatccttt catcttgtag cctataaaaa ttgcggtatt gatggctatt acacccggag
     3961 ccgactgagc catggcaagc atatcaataa attcacgcgc atccatccat tttttgtttt
     4021 ccaccacttc tttctgaatg agtggaatca ttgcatatcc gcctccgata gtgaacgaac
     4081 cgatgcggac aaaagtcata aacatttgcc agtacagacg tgagccgctc ttctctcccg
     4141 aatcggaatg attgagtttt ttctttggga gttttcgttt atcttcagtc actgtggatt
     4201 tctggttttt ataatttttc aattttctga aaaagtaaca tttaacaacg cttcaaaaaa
     4261 aagtcaaata cttctttttt cttcatcatt caacgcctga gaagtcaata ttctgcttca
     4321 gtttatcata cccggcccgt tgaatggcag aatatttgaa aatccggtta aattcttcct
     4381 gagtgagatt tttccagtca tctgaattcc aattgattac ttcttgcaac ggttgaaatt
     4441 cctgatgtct gtttggtttt gcgcggcttt tgttccacgg acaaacatcg ttgcaaatat
     4501 cgcatccata tgcgtagccc gaaagtttgc tccggatttc ctgagttatt tctcctttct
     4561 tttcgatggt ttggtacgaa atgcacttcc gggcatcgag cccttcattt tctgtcagtg
     4621 cacctgtagg gcagctatcc agacactttc tgcaggttcc gcagcgattt ttcaccggct
     4681 cgtcatacgc cagttccttg tttatcaaaa ggattccgat aaagcagaaa gagcccagcg
     4741 actcattaat cagcatttta tttttgccta tccatccaag tccggcttga gctgcccagc
     4801 gacgctcaag aatgggagcc gaatccacaa agcgatgctg ttgagatgga ttgaaacaat
     4861 ctttgcccca tttttcttta attctgtttt ctaaaagaag caatttttcg cggataacag
     4921 tatgataatc aatccggtta aaggcatatt tggaaatttt tggccaggca gcctgctgag
     4981 cagaagccgg aaagtaattc ataagtgtta ccacaactga tttacagcct tccacgagtt
     5041 tggtcggatc agtccgcttc tcaaaattcc gctccaggta gctcatctca ccattgaatt
     5101 catcgccgag ccacgatttc agaaaaagtg catcttcttc caaaaaaccg gctttggcta
     5161 taccacatgc atcaaaaccg atttccaggg ctgtattttt gataaattca gtcaaattct
     5221 gagatgaatt aaaaattacc tgagttcgat attaattttg tcagtagggc aactatatca
     5281 atagaatgaa ttaatcgtat ataattttac cgcagggaat acatccccta cggggaattt
     5341 ggtcctattt aataccaatt ctattgatat tatttcccta taggaaatta aaaatgcttt
     5401 caatcatgca aaatttctat tccttatatc gaactcacgt taaaataaaa attcgatacc
     5461 ttatgagtat cgaattcatt atattctaga gtactttacc caaatcagct tccatactct
     5521 ttatacttct atatcttcca attatgttac cttctttgct tatcagatac atggtcggga
     5581 tggcctgcac gttgtatgat ttggcagcta ttccggtttc atcatgtaca caagtccagg
     5641 gtatgtttga tacggaacgt tgccaaagca ttttactctg atccaccgag acctgataga
     5701 tttgaagtcc tttcggtgaa tattttgtaa atatttcgcg caatgcaaat gtgtaatcaa
     5761 tgctcgtttc ggttccgtag gctgagaaat caagcaaaat cggctttccg ataagcgatg
     5821 agagcttctg aggataaccg tttttatcct tgagttcaat ctctaaaaaa ccggtttttt
     5881 gaatttctcc gatgcttccc gggatatttt gccttcttgc cattctgtct tccctgatgg
     5941 cgctgagcac caggttatac aggcttttgg atcgttcata ttccggcata atggaatgaa
     6001 atgaagtggc aactgcctga taatacggac gttcggcttt tacataggga gaaaaaatat
     6061 aattcccgct tatctgctga tacaaagcaa aataggctgc cattgatccg ggatttttca
     6121 gtataattcc ctgagcaaat tttttgtgct cttccactcg catctgtaat gaatcggcca
     6181 gagattttga tttttccaca tcttttacag ccgggtaagc atcaaattgt ttttgcagtt
     6241 caatgaccga ttttcgtaat gcctgtatgt ctgcagtctg cacagatccg gtaatagccg
     6301 atgaaattaa atcggatgca gaaccttgaa tttcaatact ttccgtcgaa tcaatcccca
     6361 aaacaagttg ctgattgtcc agtctaagcc ggtagagttc gggataaaca ggtacggctg
     6421 tacggaattt aaaggtgcct tccttactga gtttaacaga atcaatcaga ctgtccttca
     6481 gcagtccggt tttctcaaaa tagagcactt taccctctgc atcggtaata tttcctgaaa
     6541 ccttgaattt tccgggattg ttacaagccg tcaacagaac aagcaataca aaaaagatac
     6601 tttttttaag catataaatt agttaagcat taatgaatca gatacttgtg aggctgtttc
     6661 ttttcctttc agcgattcta caattttcag tgcaaactga acggccacgc cgggaccttt
     6721 gccggtgatc acgtttccgt cttccaccac actttttttg gaaataacag ctccggtcag
     6781 tttcccttca aatcccggat agcaaatagc ttccttgcct tccaaaatgc ctaattttcc
     6841 gagcactgaa ggtgaagcac agatggcagc cagctttttg ccttcgctat gttgtttttt
     6901 aaggagttca tttacaccct gatgtgccga aagattcagc attccgtcat aaccgcccgg
     6961 caacacgtaa taatcattct ctccaaaagt ggtttcagtg aaaagctgat cggcttctac
     7021 tttgattcca tgtgctcctg ttactgtttt actgtctgaa atagagacag ttaccacatc
     7081 aagttctgct ctccgcaaaa tgtcaatcgg cgctatggct tctatttcct caaaaccatc
     7141 ggcaagaaaa ataaaaactt tcttcataaa ttatttgtgt taaaaaagtt aatattgacc
     7201 tgattatttc aatttgaaat tataggtaat ggtccccaca gacactccgc taccgcttga
     7261 aaaacgtgta gttcgggcgg cgctgagcgc agccgaacgg gtttgagcat ccgaaatggt
     7321 ggttggtgag cctaccgtgg cacttgtaac ccgcccggaa gcatccaccc gaatgcttac
     7381 agtaattttt ccctccacat tattcgggta ggaaggtgta gcgagcgttc cgatgagcga
     7441 acgtccgctc aacgaccatc cgtgaccgcc tgaagttccc gaaccggccg gatttccctg
     7501 gcgtgtatct ccttcggtgg ttccgctgcc cgtgcctcca ccggatccaa acgcactccc
     7561 caccaggttc tcgctccttg cagcagcttc ctgccttctt ttggcttcgg ctgcagcagc
     7621 agcttcgcgg cgttgcagct cctgttgttg caattcctgc tctgttattg ttacgggttt
     7681 tgttttaggc tttgtctgct gctttggaac aacaaccgat ttttcggttt cctgagtgat
     7741 tagttcctgc tgttcagaca cttgtttggg aatcggaggt gaagacattt cttccacacc
     7801 ggctatcatt tcgccaaagc ccattccatc aatattatca ccaaagctga ccatgatccc
     7861 ctcgtcttct gtaggtctga ccacagcagg cataaataca aaccagagta tcaaaaaagt
     7921 gagcagacag aacagcgctg agcccagcat tccatatatc tttgattttt ccaaaaccta
     7981 ctgattatca acgttttttg aatccgattc gccggttgaa gccgatgtgg ctataaccag
     8041 tttcagctta tttttattgg caatatcgag tacccttacc acttccttat acgctatttc
     8101 ctgatcggca taaagtgcca gaaacgtagc ggtatcgcgt gatatcagat tcatgatttg
     8161 ctcctcgagc atctcaggtg atacttctac tgctttttct ttatttcctg caatgtagta
     8221 tttgccttcc ttatcaatcg atactctggc cacaacttgt tcggttgcgg ttttggcgcc
     8281 gctttgaggt aagtttattt taatgtcatt gggagtagtc attgtggacg atatcacaaa
     8341 aaacagcaac agcagaaaaa tgatgtcggt catggacgac atcgggaatc cgctttccag
     8401 tctacttctt ctttttaatg ccataatatt tcagttttga gttctgagtt caaagttctg
     8461 agtactactt actcaaaact cattgctcga aactaatcat ttcgatggtt cattcaataa
     8521 atccatgaat tccagcgttt tggattccag gttgcgtacc acggaatcta cccgtgaaat
     8581 gaggtaatta taggcaaaat aagccagaat accaactata agtcctccca ctgtggttac
     8641 catggcctga tacatacctg atgaaaggtc aattagctgt actactccgg ttgagttctg
     8701 agtcatattg taaaacacga gtaccatacc cgtcacagtg ccgagaaatc caagcatggg
     8761 agcaccgccc gaaatagtgg ccagaataac caggcttttt tcgagtttgg aaatttccag
     8821 atttcctaca ttttctacgg caaggagaac atcgttcatg gggcgaccca ggcgacttat
     8881 tcccttctca atcatccgtg aagccggcga atcggtatcg cggcagagtt tctgcgctga
     8941 gtcaattttc ccgtcgagga tatagtcctt gatgcggttc ataaaggatt tatcttcttt
     9001 ggatgctttg ttgattgtta tcagtctttc tatgaaaata tacactgcaa gcgctaacat
     9061 gataccaaga attatcatta tcgggcctcc gtatactgtc atctgccaca aattcatttt
     9121 ttcaggcact gcctgtgctt cagcggtaac tgcttgacta attgtctgtt gtaataaaat
     9181 ctgcaaattc attttgtata tttattgaat gatggaatgt atattttaat gattgtattt
     9241 tttctgatta aatttctgtc aggcgtgctt tgtattgtct gatggtttct tcgagcccca
     9301 gataaagtgc atcggctatg agtgcatgac caatggatac ttcgtccagc cagggaatat
     9361 tttggtgcag aaaagccaga ttttcgaggt tcaggtcatg ccctgcgttg atgcccaatc
     9421 cgagccggtg agcttctctg gccgcctcta caaacggttg aaccgctgca aatttatcat
     9481 ttttataggc agccgcataa ggttcggtgt agagttctat tctatcggta cctgttttgg
     9541 ccgcatactc aatgttggca aggtctgtat cgacaaaaac agacacccgg attccttcct
     9601 tttgaaattc actaatcact tcccggagga aaatcagatt ctttcttgta tcccatcccg
     9661 catttgatgt cagtgcatca tgcgcatcgg gcacaagggt tacctgagct ggttttattt
     9721 ctttcacaag tttgataaat tcgggaatag gatttccttc gatgttgaac tcggtagtca
     9781 ataccggctt caaatcatac acatctgcat agcggatatg tctttcgtcc ggacgcgggt
     9841 gaacggtgat gccttcagca ccaaaacgct cgcagtccaa tgcaactttt accacattgg
     9901 gcatatcgcc gccccgcgaa tttcgcaaag tggctatttt attgatattt acgcttaatt
     9961 ttgtcatttt tcgaagagaa attactcaaa tcattgactc cgcaaaagga ttcaaaataa
    10021 atttgtgtgt ttcaattcca ttttgttcct ttgtaaaccg taagagttca acgaaatgca
    10081 aatttaactc aaataaatga agtaatggta taaaacggct tttctttcga attttttaaa
    10141 ctttttgaaa acaaaatcaa ccgatgcaaa ttttatacct tttcgtgaag aattattgtt
    10201 cctttttgaa agttccggat cattttagct gataattctt aataaactgt cttaaaacgc
    10261 tactttttta tttttcataa catactttat ggaatcaaaa gaaacaaaac gcattgcgct
    10321 tttcggaaat ctccaccgca ccgaaatgga gaatgagcta ctcgaattag ttcagttttt
    10381 ccatcagatg aatgtggttg tattgctcga tgaatatctt cagctttacc tggatgaaaa
    10441 atttcataag tcttttgaca gggtggaact gattaccgat gataatttca gtgcagatct
    10501 ggctctgagc atcggtggag acggtacttt tctgaatact gctgcccgcg tgggcaaaaa
    10561 ggatattccg atactcggta tcaatacagg cagactcgga tttctggctg atgtggcaaa
    10621 agatgaaata ataccggcgc tgcaagctat tttcaagcat gaatacacca tcgaaaaaag
    10681 gagtgtgctg aaattacaaa cgctggacgg ttcacgattg gattctccct atgcgctcaa
    10741 cgatattgcc atttccaagc aggacagcag ctcgatgatt atagtgaatg cttacgctaa
    10801 tggtgaactc attaacattt atcaggccga tggattgatt atttccactc caacaggctc
    10861 cacggcttat tcgatgagcg ccggaggacc gatagtggtg ccgcaggcga gaagttttgt
    10921 gatttcaccc gtggcttcgc atagtctgaa tgtacgtccg ctcattgtgc ccgacgattg
    10981 ggaaattgac cttcaggtga taagccgcag caacagctat ctggtttcaa tcgacggacg
    11041 ctccaagatt ctggatcaca gcacccggct acgactcaag aaagctgact acagcattca
    11101 ggttcttaaa ccgctgaacc atactttttt tgaaacgttg aggaataaac tactctgggg
    11161 ggccgatgtg agaaattgac tttttggtga acatcaatct tttacttcaa ttaccggaat
    11221 aagattacgt cttgagatgc cggcaggagc agttgctacc ctgtcgaaat gaggaatttc
    11281 acctttaaat gtacctttca gctctgtttt tccatccagt gtaaaattgc actgaatatc
    11341 atatcttcca ctctctgtga cagttacatt catggtaccg tcagtcacat aggttacttt
    11401 tgattttgtt ggatcggctt cgatgtaata catgtaagct ccggatggta tgggctcgcg
    11461 gttatcttca tattttttcc ctgcaaaaaa agtgaaaggt tcgccggttt cggccatccg
    11521 gtaagttccc gctgcgagca gcgtatccgc cggagcactg aaaatatcct gaatgataag
    11581 atattgaccg gttccgtcga gttcattatc tttgttgatg actaattttt cggtaaaaag
    11641 atgcaacgtg agtacgttgt tttgaacgcc gtaattggtg taataatttc cgtaaaaatc
    11701 ggcgagtccc caggtaaaag caggagcagt attgtactga tatggcgctg gcggttctgc
    11761 cggcttgcag gaaaatagcg ccacgaccaa aagcgaaaac agtattgatt ttatctggtt
    11821 cataaaaaaa acatttattt aacgtgagtt cgaaataatt tttgtccgta ggacatctat
    11881 gtcaatagaa tgaatttatc gattatattt ttcccgtagg gaatatatcc cctacgggga
    11941 atttggtcct gtttaatata ttttactatt gatattaatt ccctatggga aattaaaaag
    12001 ctttcattca agcagatgtc cttattctta tatcga
//
LOCUS       DAORKP010000063        11978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_543_length_11978_cov_9.621991, whole genome shotgun sequence.
ACCESSION   DAORKP010000063 DAORKP010000000
VERSION     DAORKP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_11978_cov_9.621991"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..788
                     /locus_tag="PLN75_05000"
     CDS             93..788
                     /locus_tag="PLN75_05000"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRG03509.1"
                     /translation="MKLNMKSGKLGMLTVLAIVIYAVMILSSFISEMDDFMLGYEMGS
                     NLGRSITDSTKQESAMKTVYFLDLKAGESISTFPDTIYNLKGAQKIPIRFDKAQILAD
                     AFSKDDKSALRLMKLLEIPLSLLMLVIVVLIPVFFIKLNISLKKEVVFDKSNIKCMRW
                     IGILLILYYFVMLTVSWIIYKTNLMMFEFAEYKIQFKQPDLIWILLGVVVLLFAEVLY
                     RGSKMKDEQDLTI"
     gene            806..1042
                     /locus_tag="PLN75_05005"
     CDS             806..1042
                     /locus_tag="PLN75_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRG03510.1"
                     /translation="MAIIVNLDVQMAKRKISLNDLSERVGITPANLSILKTGKAKAIR
                     FSTLEAICKELDCQPGDILEWNEDVEPDLHLHSP"
     gene            1240..4044
                     /locus_tag="PLN75_05010"
     CDS             1240..4044
                     /locus_tag="PLN75_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRG03511.1"
                     /translation="MIAFLGATAQQMPPIPLDREVKIGKLDNGMTYYIRKNKLPENQA
                     NFYIVQKVGSMQEEENQRGLAHFLEHMAFNGTKNFPNDKTGKGIIPYLESVGVKFGQN
                     LNAMTGFDQTIYLINNVPTTRQAVLDSALMVLHDWSGFLLLRDKDIDKERKVIHEEWR
                     TRQNTNMRMLESLLPTLFKGSKYAQRMPIGTMEVVDNFQPDVLREYYKKWYRPDLQGI
                     IVVGDIDVDKMESNIKTMFADIKAPVNPAERIYEQIPDNDEPLIGIAKDKENPMTNVL
                     LMFKRNGIPADKKAGMDYLIYDFANKMVEKMLTNRLEEILQKENPPFAYTEASSGQYF
                     GLAEKTDALQMVAISNPGKTDLAIETLMREAKRAKDFGFTAGEFERAKATHMSNLEKL
                     NNERDKQRSDYYVNEYVNHFLRNEPIPGIADKFATMQQLIGYIPLEAVNQIAKQLISD
                     NNRVLAVLGTENENYSTETQLSELIKKVDGETLEAYVDNTINEPLMADMPAKGSIVKE
                     ETKGDEIIWTLSNGAKVVIKPTNFKDDEVLISGFAPGGSSMISNNYLSEIKMMGEEMK
                     MILGETAISVGGLGKFSANELKKALAGKNISMDFVLKTFSENISGKSNIKDLETAMQL
                     LYLNFTNVRDDAQTYASFTSRANAVFANLGSNPMVAFSDSVMNAMYMNNPRGRIINTE
                     NIQKADYQNMLKLYRERFANAGKFVFTVVGNVKPADFKPLVEQYIASIPGKAEKSKFN
                     PDAMKMRKGKFENHYTRKLETPKASSALIYSGKLKYNLQNSIQLDALKQVLDIQLTEK
                     IREKLGGTYGVQVMAEQDKEPVEAFNIQMQFDTEPNRRVELVNAIKQAVDELQTIGPK
                     SEEIQKIKEFMTKQHADDLKKNEFTLNALNRKYRYGADFVSNYQNYVDMLTVQSMKKF
                     AKNLFKQGNRVEVSMTSESK"
     gene            4123..4524
                     /locus_tag="PLN75_05015"
     CDS             4123..4524
                     /locus_tag="PLN75_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03512.1"
                     /translation="MLREDYIMRLFQLFSESLAKWLSKRKSENSDLIQSFNSEVVKPY
                     LEQDIQFFETKSRDQLVEYFNHIYPDKYERLTRLEILSEVLYQRATLETDSNLQQNIF
                     NTTLNVLQYINSIDKTYSLARENRISDLLHR"
     gene            4606..6093
                     /locus_tag="PLN75_05020"
     CDS             4606..6093
                     /locus_tag="PLN75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HRG03513.1"
                     /translation="MLIADLLKQEFRKGFRSQAFYRGLAVKLFMGFMTLYFAAIFITI
                     GFSLGKILEEVHDTRTPLELINGASVYIILAALTIRFFMQSLNTLNLQAYQSLPVKRS
                     TLVNFILLKPLFSIGNYLTLLVVIPFAVRSVSAWHSGWVALQFVVVCILIIWMDIWLA
                     SYLKRRFGSDLRAFVVILLILAGVAALEYFKVFSVFGFSMKIFGFVMMNPLGLVVALL
                     VSGMAYGLNLMFFSKNYYPEKFNEKLSRGENKVTGGFSFLEKYGTIGELIQLQIRLIF
                     RHKRTKSLIYMSAVFMLYGLLFYTNEMYSGSPAWLIFCGLIVTGMASLTYGQWVISWE
                     SNYFDAILTKNIPAKTYIKVNYFILIAFNVISFLLSTPYLLMGTKFFFLHVAMFLFNT
                     GINAFLLIFTATFNNKRIDLSARSMFNYQGTTYKSFLIILPVMFFPMLITFILANFLS
                     FNMILLVLGLLGLSGVVSIPYQMKLCTQQFVRRKYALAEGFRESE"
     gene            6165..6860
                     /locus_tag="PLN75_05025"
     CDS             6165..6860
                     /locus_tag="PLN75_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG03514.1"
                     /translation="MITISNLTKKYNGTTVLDIPALNIQPGESFGLVGNNGAGKTTLF
                     RLILDLIEANSGEVTIKNQPVARRDEWKSYVGSFLDENFLIDFLTPDEYFAFVGKLYN
                     KSEGDISAFLEKFSEFFNGEVTGNKKLIRELSKGNQKKTGIAAALLGDPQILILDEPF
                     TALDPSSQIRLKRILNELQETKNTTMLISSHDLNHVTEVCKRTVVLEKGKVVRDIATS
                     DDTLKELESYFAV"
     gene            6953..8371
                     /gene="ahcY"
                     /locus_tag="PLN75_05030"
     CDS             6953..8371
                     /gene="ahcY"
                     /locus_tag="PLN75_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRG03515.1"
                     /translation="MNLELFSKLPYKVANIQLADFGRKEIELAEKEMPGLMALRAKYG
                     RSKPLKGARIMGSLHMTIQTAVLIETLVELGAEVRWASCNIYSTQDHAAAAIAAAGVP
                     VFAWKGETLAEYWWCTLQALTFEGNKGPTVIVDDGGDATMMIHVGVKAEKDASVLDKI
                     AEGEDEKELYSILKATLNEDATIWNRMAPEIRGVSEETTTGVHRLYQMMEEGSLLFPA
                     FNVNDSVTKSKFDNLYGCRESLADGIKRATDLMLAGKVVVVCGYGDVGKGSAKSMRAY
                     GARVMVTEIDPICALQAAMEGFEVVTVEDSLAQGNVYVTTTGNKDIIRIEHMEKMKDG
                     AIVCNIGHFDNEIQVDKLKKYPGIELVNIKPQVDKYIFPDGHSILLLADGRLVNLGCA
                     TGHPSFVMSNSFTNQTLAQIELFTKDYPVDVYRLPKHLDEEVARLHLDQLGVKLTRLT
                     PDQAEYIGVNADGPYKSEHYRY"
     gene            complement(8491..9300)
                     /locus_tag="PLN75_05035"
     CDS             complement(8491..9300)
                     /locus_tag="PLN75_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRG03516.1"
                     /translation="MAAQKAFPARNIPTADTTNIYNEMLNDETDDLMENHPAGDLYND
                     IWSSEKLNPYKVSINSLPDSIQIDCSDFTMPVSTGRVSSKFGPRRYRYHYGIDIALPS
                     GNDVVSSFAGKIRVVDYEPGGYGNYVVVRHDNGLETVYAHLSAVTCKVNDRIKAGDKL
                     GLSGNTGRSTGPHLHFETRYIGNAFNPSSLINFDSKQVYAHNYTLTKRKNFGYQQHKA
                     NTVQLAKYYKVRKGDNLSVIASRNGTTVNKLKKLNGFKSSKVKYGQRIKIR"
     gene            complement(9415..9876)
                     /gene="ndk"
                     /locus_tag="PLN75_05040"
     CDS             complement(9415..9876)
                     /gene="ndk"
                     /locus_tag="PLN75_05040"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRG03517.1"
                     /translation="MEQTLVILKPSALQRALVGEVIKRFEQKGLRLAGIKMIQLSDQI
                     LNEHYAHLADRPFFKRIKDSMMITPVIVTCWEGKDSVRVVRTMTGSTNSRDAVPGTIR
                     GDLSMSLQENIVHTSDSVENAREELARFFSDDEIFDYENLLTPYLYANDEI"
     gene            complement(9990..>11978)
                     /gene="recG"
                     /locus_tag="PLN75_05045"
     CDS             complement(9990..>11978)
                     /gene="recG"
                     /locus_tag="PLN75_05045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRG03518.1"
                     /translation="HYPYRYVDRSRFYKIRELREDMPFVQIKGKFVRFEKVGEGKKMR
                     LSAMFSDGENILELVWFRGVRFVTDKYKTGVEYVAFGKPTLFNGKFNLVHPDIEDASK
                     LPPPEKMGFQPYYNTTEKMKNHFLNSKAIQKIIYPLIGKLEAGIPETLPAYVLQQFKL
                     MNLTESLINIHFPKNDSNLTKARQRLKFEELFYIQLDILRQRKWREQRFSGFIFSRIG
                     KYFNEFYSSHLPFALTNAQKKVIREIRTDMSSGRQMNRLLQGDVGSGKTVVALLTMLI
                     ALDNGYQACLMAPTEILANQHFTTISEQLRGINVNVSLLTGSTKKKDRESIHEQLLNG
                     EMQIIIGTHALIEDTVQFKNLGYVIIDEQHRFGVAQRSRLWIKNEHPPHILVMTATPI
                     PRTLAMTLYGDLSVSVIDELPPGRKAIQTYHYYESKRNSLLGFISKQINAGRQVYVVY
                     PLIQESEKMDYRHLEEGYAYLKEVFPNYMISMVHGKMKPAEKEAHMQKFVTGETQLMV
                     ATTVIEVGVNVPNASVMIIESAERFGLSQLHQLRGRVGRGADQSYCILLTGVKLSSDS
                     RKRMEIMTRTNDGFEISEADLQLRGPGDMEGTQQSGIPFELKIADLAKDAQMLEIARN
                     TAWDILEEDPTLSKPENRMLVLQLKKMKTNAVNWSAIS"
BASE COUNT         3462 a         2537 c         2611 g         3368 t
ORIGIN      
        1 ataaataaat tttgtttttc ggaaaaagta gtttagattt gcaccgtgaa tcagatttag
       61 tatattatga attttactca ataaatcctc ccatgaaact caatatgaaa agcggaaaat
      121 taggtatgtt gacagtactg gcaatagtta tctacgcagt aatgattctg tcatcattta
      181 tttctgaaat ggatgatttt atgctggggt atgaaatggg aagcaactta ggcaggagta
      241 ttactgacag tacgaaacag gaaagtgcga tgaaaacagt ttatttcctt gatctgaaag
      301 ccggtgagag catcagcaca ttcccggaca ccatttataa tctcaaagga gctcagaaaa
      361 tacccatccg gtttgacaaa gcgcagattc tggccgatgc attttcgaag gatgataaat
      421 cggccctgag actgatgaaa ctgctggaaa taccgctttc attgcttatg cttgttattg
      481 tagtactaat acctgttttt ttcatcaaac tgaatatcag cctgaaaaaa gaagtggttt
      541 ttgataaatc aaatattaaa tgtatgcgct ggattggtat tctgcttatt ctttattatt
      601 ttgtgatgct tacagtgagc tggattatct ataaaacaaa cctgatgatg tttgaattcg
      661 ctgaatacaa gattcagttt aagcagcccg acctgatttg gattttgctt ggcgtggttg
      721 tgcttttatt tgccgaagta ctgtaccggg gttctaaaat gaaagacgaa caggatctca
      781 ctatataaaa aagcaaaaga agtaaatggc aataatagta aatctggatg tgcagatggc
      841 aaaacggaaa atatcgttaa acgatctttc cgaacgggtg gggattaccc cggccaatct
      901 gtcgatactg aaaaccggaa aagcaaaagc catccgtttc agtacgctcg aagctatttg
      961 caaagaactc gattgtcaac ccggagatat tctggaatgg aacgaggatg tagaaccgga
     1021 tttacacctt cactctccct gaaaaaaaaa taccgaaata cgttaacacg gttcgaatat
     1081 catttttata agtttctttt ctgacctgtt tttattcatt cagcaaaaaa aacagattaa
     1141 aaaccggatt atgctgaaaa actaatatgt aacaaaaact gtttttatta aaaattattc
     1201 ttaaagatga aaaaactaac attattatct ggactaatta tgattgcttt tttgggtgca
     1261 acagcacagc aaatgccacc tatcccgctt gacagggaag tgaaaatcgg gaaactggac
     1321 aacgggatga cctattacat ccgcaaaaac aaactccctg aaaatcaggc taatttctat
     1381 atcgtacaga aagtgggatc catgcaggaa gaagaaaatc aacgcggatt ggctcatttc
     1441 ctggaacaca tggcattcaa cggcactaaa aattttccga acgacaaaac cggaaaagga
     1501 attatacctt acctggaaag tgtaggggtg aaattcggcc agaacctgaa tgctatgacc
     1561 ggttttgatc agaccattta cctgataaac aacgtgccaa ccacccgcca ggctgtgctt
     1621 gacagcgcat tgatggtgct gcatgactgg tcgggcttcc tgctgcttcg cgacaaggac
     1681 atcgataaag agcgcaaagt gattcacgaa gaatggagaa cacgacagaa taccaacatg
     1741 cgaatgctcg aatcgctgct tcccacactt ttcaaaggga gcaaatatgc tcagcggatg
     1801 ccaataggca ccatggaagt ggtggataat tttcaacccg acgtgttgag agaatattac
     1861 aaaaaatggt atcgacctga tttacaggga ataattgtag taggagatat cgatgtggac
     1921 aaaatggaaa gcaacattaa aaccatgttt gccgatatca aagcaccggt gaatcccgcc
     1981 gaacgaatct acgaacaaat acccgataac gacgaaccac tcatcggaat agccaaagac
     2041 aaggaaaatc caatgaccaa cgtactgctc atgttcaagc gaaacggtat tcctgctgat
     2101 aaaaaagccg gtatggatta cctgatttat gactttgcca ataaaatggt ggaaaaaatg
     2161 cttacaaacc ggctggaaga aattctacag aaagaaaatc caccgtttgc ttataccgaa
     2221 gccagtagtg gacaatattt cggattggcc gagaagaccg atgcgctgca aatggtcgct
     2281 atttcaaatc ccggaaaaac cgatttagcc atcgaaacac tcatgcgcga agccaagcgg
     2341 gcaaaagatt tcgggttcac agccggcgaa tttgaacggg ctaaagcaac acacatgagc
     2401 aacctggaaa aacttaacaa cgaacgcgac aaacaacgca gtgactatta tgtaaacgaa
     2461 tatgtgaatc attttctgag aaacgaaccc ataccgggca tagccgataa atttgccacc
     2521 atgcagcaat tgattggtta catcccgctc gaagccgtta atcagatagc aaaacaactt
     2581 atttcagaca ataaccgtgt tttggctgta ctgggcaccg aaaatgaaaa ctattccacc
     2641 gaaactcaac tttcggagct gattaaaaaa gtggatggag aaaccctgga agcctatgtg
     2701 gataatacaa tcaatgaacc tctgatggcc gatatgccgg ctaaaggcag catcgtaaaa
     2761 gaggaaacca aaggcgatga aatcatctgg acattgtcca acggagcaaa agtggtgatt
     2821 aagccgacta atttcaaaga cgacgaagtg ttgatatccg gatttgcccc cggtggatcc
     2881 tctatgataa gcaacaatta cctttctgaa ataaaaatga tgggcgaaga aatgaaaatg
     2941 attttgggcg aaacggccat cagcgtaggg ggattgggta aattcagcgc caatgagctg
     3001 aaaaaagccc tggcaggaaa aaatatcagc atggatttcg ttctgaaaac tttctctgaa
     3061 aacatcagcg gaaaatcgaa catcaaagac ctcgaaaccg ccatgcaact gttgtatctc
     3121 aactttacca atgtacgcga tgatgctcag acttatgctt cgtttacatc acgtgccaat
     3181 gctgtgttcg ccaacctggg aagcaatccg atggtggctt tcagtgattc ggtgatgaat
     3241 gccatgtata tgaataatcc gcgcggaaga atcatcaata ccgaaaatat ccaaaaagct
     3301 gattatcaga acatgttgaa actttacagg gagcgctttg ccaatgccgg gaaatttgta
     3361 ttcacagtgg taggtaatgt gaagcccgca gatttcaaac cgcttgttga acaatacatt
     3421 gcctccatac ccggaaaagc cgaaaaatca aaattcaatc cggatgcaat gaaaatgcgt
     3481 aagggaaaat tcgaaaacca ttatacgcgg aaactcgaaa ctccgaaagc ctcttcagcc
     3541 ctcatatatt ccggtaagct gaaatacaac ctgcaaaact ccattcagct ggatgctctc
     3601 aagcaagtgc ttgatattca gttgactgaa aagatcaggg aaaagttggg tggaacctac
     3661 ggggtacagg taatggctga gcaggataaa gaacctgtgg aagcattcaa tattcagatg
     3721 cagtttgata cagagccaaa ccgccgcgtg gaattggtca acgccatcaa acaggctgtg
     3781 gatgagttgc agactatcgg tccgaaatcc gaagaaattc agaaaatcaa ggagttcatg
     3841 accaagcagc atgccgacga tctgaagaaa aatgaattta cgctgaacgc gctgaacagg
     3901 aaataccgct acggagccga ttttgtgagc aattatcaaa actatgtaga tatgcttacg
     3961 gtacaatcca tgaagaaatt tgcaaaaaat ctcttcaagc agggcaacag ggtggaagta
     4021 tccatgacca gcgaaagcaa ataaaatcat atacctaaac tttctgaaaa acggcacctg
     4081 acaacttgtg ctgtgccgtt tttctttttt tcaaataaca atatgctcag ggaagattat
     4141 attatgcggc tttttcagct atttagcgag agtctggcta aatggctgtc aaaaagaaaa
     4201 tccgaaaatt cggatctgat acaaagtttc aacagcgaag tggttaaacc atatctggaa
     4261 caagatattc agtttttcga aacaaaaagc agggatcaac tggtcgaata ttttaaccat
     4321 atctaccccg acaagtacga acgtctcacg cgtctcgaaa ttctgtcgga agtcctgtat
     4381 cagcgggcga cgctcgaaac tgacagtaat cttcagcaaa acattttcaa tacgacctta
     4441 aatgtgttgc aatatatcaa ttctatcgat aaaacctact ccctggcacg tgaaaaccgg
     4501 atttccgatc tgctgcaccg gtagaattcc ggtttttcaa tacataatgc atgttattta
     4561 ctttcaatta attttttaac ttttggaaaa taaacaaaaa atcctatgtt gatagctgat
     4621 ttgttaaaac aggaatttcg gaagggattc cgatcgcagg ctttttatcg tggtctggcc
     4681 gtgaagttgt ttatgggctt tatgacgttg tattttgcag ccattttcat cacaattggc
     4741 ttttcgctcg gcaaaattct tgaagaagtg catgacaccc gcactccgct cgaacttatt
     4801 aacggagctt cggtttacat tattcttgca gctcttacta tccggttttt tatgcagtca
     4861 ctcaatacgc tcaacctgca ggcttatcag tcgctgcccg tcaaacgttc cacactggtg
     4921 aattttattt tgctgaaacc gctttttagt ataggcaatt acctcaccct gcttgttgtc
     4981 atcccatttg ctgtgcggag tgtatcagcc tggcattcag gctgggttgc acttcagttt
     5041 gttgttgtgt gtatcctcat tatctggatg gatatctggc tggcttccta cctcaaacgc
     5101 cgcttcggat ccgatttgag ggcattcgtg gtaatactcc tgattttagc cggtgtagca
     5161 gctttggaat acttcaaagt cttttctgta ttcggttttt caatgaaaat ttttggattc
     5221 gtaatgatga atccgctcgg attagtggta gctctgttag tatccggtat ggcatatgga
     5281 ttaaatctta tgtttttttc aaaaaattat taccccgaaa aatttaacga aaaactgagc
     5341 cggggtgaaa ataaagtcac cggcgggttc tcgtttctcg aaaaatacgg tacgatcggt
     5401 gaattgattc agttgcagat ccgtttgatt ttcaggcata aacgaacaaa atcattaatt
     5461 tacatgagtg cggtttttat gttgtacggc ctattgtttt ataccaacga aatgtattcc
     5521 ggaagccccg cctggctcat tttctgcgga ctgatagtca ccggaatggc atcgctcacc
     5581 tacggacagt gggttataag ctgggaaagc aactattttg atgccattct caccaaaaac
     5641 attccggcaa aaacatatat aaaagtcaat tacttcattc tgatagcttt caatgtgatt
     5701 tcgtttttgc tctctacacc ttacctgctg atgggaacca aatttttctt tctgcatgta
     5761 gcaatgtttt tgttcaatac gggcataaat gcctttttac tcatattcac agccacattc
     5821 aataataaac gaatcgacct ctcggcaaga agtatgttca actatcaggg tacaacctac
     5881 aaaagttttc tgattattct tccggtcatg tttttcccga tgctgattac tttcatactg
     5941 gctaattttt tgtcattcaa catgatattg cttgttttag ggctgttggg actttccggt
     6001 gtcgtatcca ttccctatca gatgaaactg tgtacgcagc agttcgtacg taggaaatat
     6061 gctctggcag aaggattcag ggaaagtgaa taacgaacca ttttcttgaa ataatttatg
     6121 taaatcaaca gattcaatac accaaataac taaaaaaacc atccatgata acgatttcaa
     6181 atcttacaaa gaaatacaac ggtaccactg tacttgatat tcctgcatta aatattcagc
     6241 cgggcgaaag ttttggcctt gtaggcaaca acggagcagg taaaaccacg ctctttcggt
     6301 tgattctaga tctgattgaa gccaacagcg gtgaagttac catcaaaaat cagcccgttg
     6361 cccgtcgcga cgagtggaaa tcatacgtag gctctttcct cgacgagaat tttctcatcg
     6421 attttctcac acccgacgag tattttgcat tcgtaggcaa actctacaac aaatccgaag
     6481 gtgatatttc agcatttcta gaaaaattca gcgaattctt caacggcgaa gtaaccggta
     6541 acaaaaaact catacgcgaa ctttccaaag gaaaccagaa gaaaaccgga atagctgccg
     6601 ctctgctcgg cgacccgcag atattaatac tggacgaacc tttcactgcg ctcgaccctt
     6661 cgtcgcagat tcgactcaaa cgaatcctca acgaactgca ggaaacgaaa aatacaacca
     6721 tgctcatctc cagtcacgat ctcaatcacg tcactgaggt gtgcaaacgt accgttgtgc
     6781 tcgaaaaggg caaagtagtg cgcgatattg ctaccagtga tgataccctg aaggagctgg
     6841 aaagttattt tgcagtataa ttcttacatt taattgaagt ttttattacc tgtaaaaaaa
     6901 tattatcttt gcattctttt ttagaagaat aaaaaacaac aacaaataaa aaatgaattt
     6961 agaacttttt tcaaaattac cctataaggt agccaatatt cagttggccg actttggcag
     7021 aaaggaaata gaactcgccg aaaaggaaat gccgggtctg atggctttgc gtgccaaata
     7081 cggccggtcg aaaccgctga aaggtgcacg tatcatgggt tcgctccaca tgaccattca
     7141 gactgccgta cttatcgaaa cacttgtaga gctcggtgcc gaggttcgat gggccagctg
     7201 caacatttac tccacacagg atcatgctgc tgctgccatt gctgctgcag gtgtgcccgt
     7261 ttttgcctgg aagggtgaaa cactcgccga atactggtgg tgtacgctcc aggcattgac
     7321 attcgaaggc aataaaggcc cgacagtgat agttgacgat ggcggagatg ccaccatgat
     7381 gattcacgtg ggcgtaaaag ccgaaaaaga tgcttccgtt ttggataaaa ttgccgaagg
     7441 cgaagacgaa aaagaacttt acagcattct gaaagccaca ctcaatgagg atgctacaat
     7501 ctggaacaga atggctcccg agattcgggg cgtgtctgaa gaaaccacaa ccggagttca
     7561 tcgcctgtat cagatgatgg aggaaggctc gctgcttttc ccggctttca acgtgaacga
     7621 ctcggtgaca aaatcaaaat tcgataatct gtacggttgt cgcgaatcgc tggctgacgg
     7681 tattaagcgt gctaccgacc tcatgcttgc cggaaaagtg gtagtagtat gcggctacgg
     7741 cgatgtggga aaaggctcgg ccaaatccat gcgtgcatac ggtgcccgcg tgatggttac
     7801 tgaaattgat cccatctgtg ccctgcaggc agccatggaa ggattcgaag tggtaactgt
     7861 agaggattcg ctggctcagg ggaatgtgta tgttaccaca acaggcaaca aggacatcat
     7921 ccggattgaa cacatggaaa agatgaaaga cggagccatc gtttgcaaca taggtcactt
     7981 tgataatgaa attcaggtgg ataaactcaa aaaatacccc ggaatcgaac tggtgaatat
     8041 caaacctcag gtggataagt acattttccc cgacggacat tcaatactgc tgcttgccga
     8101 cggtcggctg gtgaatctgg gctgtgctac cgggcatcct tctttcgtga tgagtaattc
     8161 gtttaccaat cagacactgg ctcagatcga acttttcacc aaggattatc ctgtggatgt
     8221 gtatcgcttg ccgaagcacc tcgatgagga agtggctcgt ttacatctcg atcagctggg
     8281 agtaaaactc acccgcctta caccggatca ggctgaatac atcggtgtga atgccgacgg
     8341 accctacaaa tccgaacatt atcggtatta attcttgttt aaattgcaga acgatcagaa
     8401 tatcaatcag aataaaaaaa ccattcggaa gtccgaatgg ttttttttac gaatatctat
     8461 attggttttt tattaaacca aaagatttga ttatctgatt ttaattcgtt gtccgtattt
     8521 tactttggag gatttgaatc cgttcagttt cttgagcttg ttcacggttg taccgttgcg
     8581 ggatgcaatt acactcaggt tgtctccttt tcgtactttg taatatttgg ccagttgaac
     8641 agtattggct ttatgttgct gataaccgaa gtttttgcgt ttggtaagtg tgtagttgtg
     8701 agcatagacc tgtttgctgt caaaattaat cagtgaggaa ggattgaaag cattgccgat
     8761 gtagcgggtt tcgaagtgaa ggtgcggacc ggtggaccgg cctgtattgc cgctcaaacc
     8821 cagtttgtct cctgctttta tacggtcgtt taccttgcat gtaacggcgc tcaggtgagc
     8881 atacactgtt tcaagtccgt tgtcatgcct caccacgaca taatttccgt atccacccgg
     8941 ttcgtagtca actactctga ttttaccggc aaaagaagat acaacatcat ttccgctcgg
     9001 aagtgctata tctattccgt aatgataacg gtatcgtctc ggtccgaatt tggaagatac
     9061 tctgcctgtg cttacgggca tcgtaaagtc cgaacaatcg atctgtatgg agtcgggcag
     9121 actgttgatg ctaaccttgt atggatttaa tttttcactg ctccagatgt cgttatacaa
     9181 gtcacctgca gggtggtttt ccattaagtc gtccgtttcg tcgttcaaca tttcgttgta
     9241 aatgttggtt gtgtctgcgg tgggaatatt gcgtgcggga aaagctttct gtgcagccat
     9301 tttcatgttt ccgggggctg cctgtacttt tactacttgt gcgtttacgg tgtttatcgc
     9361 aattatgctt actagtgttg taagaattaa gtgtttaaac gtcattagct gtttttaaat
     9421 ttcgtcgtta gcgtatagat acggtgtcag taagttttca tagtcgaaaa tttcatcatc
     9481 actgaaaaat cttgccagtt cctcccttgc attctcgaca gagtcggagg tatgtacaat
     9541 attttcctgc aaactcatac tcaagtcacc tcttatggtt ccgggtactg catcacgact
     9601 attggtagag ccggtcatgg tgcgtacaac acgtacagaa tcctttccct cccagcaagt
     9661 tacaatcaca ggagtaatca tcatcgaatc tttaatgcgt ttgaagaaag gacggtcagc
     9721 cagatgagcg taatgctcat tcaaaatttg gtcagacagt tgaatcatct taatcccggc
     9781 gagtctgagc cctttttgtt cgaagcgttt gatcacttct cccactaaag cgcgctggag
     9841 tgccgatggc ttaaggatta caagtgtctg ttccatttta aaataatgtt gaatttttat
     9901 tcaaatatta atctgtcgtc aaaagtaccc aaaacaaatt agatatcaaa ttgttccggg
     9961 ggaaaaaaat atttttttaa cttatttttt caactaattg cactccagtt caccgcgttg
    10021 gttttcatct ttttcaattg caatacgagc atccggtttt ccggcttact taacgtggga
    10081 tcttcttcca gtatgtccca ggctgtattt cgtgctattt ccagcatctg agcgtcttta
    10141 gcgaggtcgg ctattttcag ttcaaacggg atgccgcttt gctgagttcc ttccatgtcg
    10201 ccgggtccgc gcagttgcag gtcggcttct gaaatctcaa atccgtcgtt tgtccgggtc
    10261 atgatctcca tccgctttcg cgaatcggac gaaagcttca caccggtgag caaaatacag
    10321 taggactgat cggcgccgcg ccctactcgt ccacgcagct gatgcagctg tgacagcccg
    10381 aaccgctcgg cgctttcgat aatcatcacg gatgcattcg gtacatttac tccgacttca
    10441 ataacggtag ttgccaccat taattgtgtt tcgccggtta caaatttctg catgtgagct
    10501 tctttttcag ccggtttcat ttttccgtgt accatgctta tcatgtagtt cggaaaaact
    10561 tctttcaggt aagcatatcc ttcttccaaa tggcggtaat ccattttttc agattcctga
    10621 attaacgggt ataccacata aacctgcctc ccggcgttga tttgtttact tataaaaccc
    10681 agcagggagt ttcgtttcga ttcgtaataa tggtaagtct gaatggcttt gcgtccggga
    10741 ggaagctcgt cgatgaccga aacgctcaaa tcgccgtaca gggtcatggc cagtgtacgc
    10801 ggaatgggag tggcggtcat caccagtata tgcggcggat gttcgttttt aatccaaagt
    10861 ctggagcgtt gggctacgcc aaaccgatgc tgctcgtcaa taatgacata acccaggttt
    10921 ttgaactgca ccgtatcttc aatcaatgcg tgtgtaccga tgattatctg catttcgccg
    10981 ttcagaagct gctcgtgaat gctttcgcgg tctttctttt tggtggaacc ggtaaggagt
    11041 gatacgttca catttatgcc acgcaactgc tcgctgatag tggtaaagtg ctggttggca
    11101 agtatttctg tgggtgccat caggcatgcc tggtatccgt tgtccagtgc aataagcatg
    11161 gtcaacagcg ccactactgt tttgccgctt cccacatcgc cctggagcag ccggttcatc
    11221 tgcctgcccg acgacatatc ggtgcggatt tccctgatta ctttcttttg ggcattggta
    11281 agggcaaatg gcaggtgaga ggagtaaaac tcgttgaaat acttgcctat tctggagaaa
    11341 ataaatccac tgaaacgttg ttctctccac ttacgctgac gcaaaatatc cagctggata
    11401 tagaaaagtt cttcgaattt caaccgttgc cttgccttag tcaggttact gtcgtttttt
    11461 ggaaaatgaa tgttgatgag cgattctgtc agattcatca gtttgaactg ctgcaatacg
    11521 tatgcgggga gtgtttccgg aattcctgct tccagtttcc ctatcagtgg atatatgatt
    11581 ttctgtatgg ctttcgaatt cagaaaatga tttttcattt tctcagtcgt gttgtagtag
    11641 ggttggaaac ccattttttc gggcggaggc agtttcgatg catcttcaat gtcggggtga
    11701 accaggttga acttgccgtt gaataacgtt ggcttgccga atgccacgta ttcaacgccg
    11761 gttttgtatt tatcggttac aaagcgtaca cccctgaacc aaacgagttc aaggatgttt
    11821 tctccatccg agaacatagc cgaaagccgc attttctttc cttcgcctac cttctcaaac
    11881 cgtacaaact tacctttgat ttgaacaaat ggcatgtctt cgcgcaattc gcggattttg
    11941 taaaaacgac tacggtctac gtacctgtaa gggtaatg
//
LOCUS       DAORKP010000064        11973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_544_length_11973_cov_8.912401, whole genome shotgun sequence.
ACCESSION   DAORKP010000064 DAORKP010000000
VERSION     DAORKP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_11973_cov_8.912401"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1401)
                     /locus_tag="PLN75_05050"
     CDS             complement(112..1401)
                     /locus_tag="PLN75_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03519.1"
                     /translation="MKRINYLFLLLGTFLLGGCSESVEETITYKVNEPVFMSADLFRK
                     SVRVTDVPQKINKQGKIAFFQGYMYISEPEKGIHIIDNRNPAKPNVIGFIELLGNADM
                     AIKDNILYADSYIDLVWFDVSSPAQPRLLGRKEEVFPTSVPSIDNNFVCDYEKSMDRN
                     NGIVIGWVVKERTEKYMRERSYWWWWRGGMLEDSAPMANSAYTGGAKGAVGQTGSMSR
                     FAIYKNNLYTVLNNQLGIFNIENPTPVKSSEDFYVGWNVETIFSYKDYMYMGTPTGML
                     IYSVADPLKPERMASITHILGCDPVVVENDVAYVTIHSGNFCGQNSNQLLIYNVKDPK
                     QPKHIVSYEMKNPKGLGIDNGTLFLCDEGLKVFNASNPLTIMDPANLLAHDKGMDGFD
                     LIAYEKTLMMIAADGIYQYDYTDLKNIKPLSKISIGN"
     gene            complement(1403..1861)
                     /locus_tag="PLN75_05055"
     CDS             complement(1403..1861)
                     /locus_tag="PLN75_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03520.1"
                     /translation="MNNNLHIKKSVLSVITVLCMLSFGCENINDVGWDEIRTAAYDRE
                     TVIPVNHTVRTTDRKYIVKFDSVFTDSRCPEGVYCFWSGVAGIRFLVEDDSESRKSLD
                     IYTLKFSHFADSATYNGLKIKLIYLDPYPSVGKKLKYDSYKAKIILSKTK"
     gene            complement(1922..3145)
                     /locus_tag="PLN75_05060"
     CDS             complement(1922..3145)
                     /locus_tag="PLN75_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRG03521.1"
                     /translation="MKNPIILLVCISLLISCDNTNPLPDGTADPINLTAVQKQRVAQD
                     NEFAFDLMNRTMADTSDENVFISPLSVSIALGMARNGAIGVTKTEMETALKMEGLTDA
                     EINEYYKLMQTTLPDLDPKTRLTIANSIWYRNSFSVKAGFLKTNTDYFNSEVAALDFN
                     SPDAVKTINKWCSDHTNGLIPEVIKTIDPAQMMFLINAIYFKGTWVKQFDKKDTRESD
                     FTNELNQRVKVNMMNITDTFAYTEDDYAQYLDMPYGNQAFSMTVILPKGTGRAADVFK
                     TLTPEKYSQVLKGLDVQKVIVSMPRFRVKNRFQLKEPLRQMGMLKAFQPNAEFDNISI
                     LKPLYIGFVQHDTYVEVTEEGTEAAAVTTIGLKNTSVPEHPYFIVNKPFTFVIREKSS
                     GIILFMGKVGNAEKF"
     gene            complement(3203..4426)
                     /locus_tag="PLN75_05065"
     CDS             complement(3203..4426)
                     /locus_tag="PLN75_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRG03522.1"
                     /translation="MKTPVLMLLLVSLLISCDNTNPVPDRTADPINLTAVQKQRVAQD
                     NEFAFDLMNRTMADTSDENVFISPLSVSIALGMARNGAIGVTKTEMETALKMEGLTDA
                     EINEYYKLMQTTLPDLDPKTRLTIANSIWYRNSFSVKAGFLKTNTDYFNSEVAALDFN
                     SPDAVKTINKWCSDHTNGLIPEVIKTIDPAQMMFLINAIYFKGTWVKQFDKKDTRESD
                     FTNELNQRVKVNMMNITDTFAYTEDDYAQYLDMPYGNQAFSMTVILPKGTGRAADVFK
                     TLTPEKYSQVLKGLDVQKVIVSMPRFRVKNRFQLKEPLRQMGMLKAFQPNAEFDNISD
                     LKPLYIGFVQHDTYVEVTEEGTEAAAVTTIGFETTSLPVNPYFIVNKPFAFVIREKSS
                     GVILFMGKLGNVGKF"
     gene            complement(4448..5773)
                     /locus_tag="PLN75_05070"
     CDS             complement(4448..5773)
                     /locus_tag="PLN75_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03523.1"
                     /translation="MSDNIKHTDEKDVFSTLIKEKLENHQLPVDESIWNAIEQKMTPR
                     RRIITPVWYWISGAAVAVVALLLIINPFYETKQIPALTGVQQTEMLKPDSSPQLQYDR
                     QIQAAEKVEDVFTALTGSNKNTENTGNNLSDQTASTSEKQLLITENRNKPYTTAPGET
                     RITETQTAEVQKPEEKVMNDAVNTVVDSVRQKKTTTGTPVTSLPDLNDYPEVPPAKHQ
                     PLKKRPLLIAAAVGTGGSLSSISQLDGGGKFYDSSPSLVKREIASNYTTVLDANDYTE
                     AKHDPPVSAGIMLVKPLNRTLSLESGLIYTYLRSEYYRPGLTNYRGVLQLHYLGVPLN
                     MRALLIDFPKWNLYVSAGGMVEKGLRSIYMQEIEDQQATANTNVKSGIDGLQWSLNGA
                     LGAEYKLRKGLSLYAEPELTYYLRNNQPRSARTEQPLTIGLNGGLRIEL"
     gene            complement(5773..6321)
                     /locus_tag="PLN75_05075"
     CDS             complement(5773..6321)
                     /locus_tag="PLN75_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRG03524.1"
                     /translation="MISDNEQLLIAGCKRGEPWARRELYEKYAPAMLSLCVRYVANRE
                     TAKDVLQDGFVKVFTHIGQYEARGPLPAWLRQIFVNTALEYLQKNEILKMNVSMDHFS
                     DSIDEFDHRVLDKLSADELLECVAELPDRSRSIFNLYAIEGYSHAEIAEKLNIQENTS
                     RSQFARARKALQQLVSDLMNKR"
     gene            complement(6566..8632)
                     /locus_tag="PLN75_05080"
     CDS             complement(6566..8632)
                     /locus_tag="PLN75_05080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRG03525.1"
                     /translation="MALVLTALAACDNNEKKTDDAPLIGKQTPVIENGLLTPEVLWAF
                     GRIGGVQVSPDETKILYTVSYYSVEQNKSNSEIFVMNVDGTDRKQITATPHRESAAKW
                     MNDSQTIAFLSNESGSSQLWTMKADGSRRTRLTETEGGINDFAFSPDESKLLYVADVK
                     YGERTVDKHPDLPKSSGIIVNDLMYRHWDEWVQTVPHPFLADFVGGMLENIKDILAGE
                     PYESPMKPFGGIEQLAWSPDGKTVAYTCRKKTGKEYALSTNSDIYLYNTENATSENKT
                     EGMPGYDQNPVFSPDGEWMAWESMENDGFESDKSRLYVLNTKTGEKKDLSARFDQSSN
                     ALSWSPDSKYIYFVSVWHGTTQVYRADVLTDSITQVTRGMHDYESVIPLKDKLIGVKH
                     SLSKPDEIYAINQTTGEEKELSFENREILEKLKMGNVEERWIATTDGKQMLTWVVYPP
                     DFDPNKKYPTLLYCQGGPQSAVSQFWSYRWNLQIMAANGYIVVAPNRRGLPGFGHEWL
                     TQISGDYGGQNMKDYLSAIDAVAKEPFVNKDKLGCVGASYGGFSVYWLAGHHQKRFKA
                     FIAHDGMFNLPQQYLETEEMWFVNWDLGGAYWDKQNATAQRSYANSPHLFVDKWDTPI
                     LVIHGEKDYRILASQGMAAFNAAVLRGVPAEMLIFPDENHWVLQPQNGVLWQRTFFGW
                     LDKWLK"
     gene            8882..9490
                     /locus_tag="PLN75_05085"
     CDS             8882..9490
                     /locus_tag="PLN75_05085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRG03526.1"
                     /translation="MIDSFADRAAYWDQPDKEKMAERFVTELHRRVTLQGSWKALEIG
                     AGTGLVGLMLLPDLASIVFEDTSASMLDVLRKKTIGMSNIDILHGEITDYNRQDIDLV
                     ISNMAFHHIEDIAHTLKHLHSITRPGAVVVIGDIRSEDGSFHRFQPIPHRGFDTGALS
                     VLFEQAGFAVDAVETYHVLRQEKIPGTISEYEQFILVAHKSA"
     gene            9675..9848
                     /locus_tag="PLN75_05090"
     CDS             9675..9848
                     /locus_tag="PLN75_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HRG03527.1"
                     /translation="MKELVAKINAEIAAFAENAALQADKGNKAAGARARKASLDLEKS
                     LKEFRKVSIAASK"
     gene            complement(10050..>11973)
                     /locus_tag="PLN75_05095"
     CDS             complement(10050..>11973)
                     /locus_tag="PLN75_05095"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRG03528.1"
                     /translation="KSPVMLTAVINKDQSVDITATFNGKKTNTKIKQMPALYKTPAGI
                     LTVSFRAGKAPNYGVPIQVVIRKPMSMVPYFRSGLAVAPTSKTTSVLNLAYTTTETQK
                     GIDFLNALVGVYNMDATADKNKEALNTQEFIDGRLAIINNELGSAERQVEDYKRSQGL
                     TDITSDVQMSLQKGSQYEQKLVEVSTQLNLVDYLDTYINEQRNRNKLIPSNVGIEDPT
                     LAATINEYNRQILERDRLLRTNTESNPVIQKLDGQIEGLRAGIESSIASVKQGLSIAR
                     RDAQSQVNYYKSQTGAAPTQERQFTEIAREQQIKSSLFLMLLQKREENALSLAATAPK
                     AKVLDEATVGRMIKPKRMMVLMIALFLGLLIPALIIFLKDLLHFRIESRADIDRYSNI
                     PSLGEIPVSASGNIAVVANENRETEEAFRMLRTNLLFTLGKDKKVVVVTSTEPKEGKT
                     FIAINTAISLSLLDKKVLLMGMDLRLPRLSEYLNIDTGHGMSQFLSGNENNILDIVQP
                     SGINPNLSVISAGAIPPNPAELIARTEFEKGMEVLRQKFDYIIIDSAPVSLVTDTIVA
                     SRVADATMYVCRANYSRKNNLRFANDLSDKKMLPNMGLVVNDVSNFHSGYGTYGSYSS
                     YGYGYNKDNKKKKRIL"
BASE COUNT         2806 a         2747 c         2840 g         3580 t
ORIGIN      
        1 cgccgctctc tctgattttt cggctcaccg gacgaggtcg cccgttagca atcttctcag
       61 cgaatagtaa gttataattt ggaaaagttt agttaaatat aacacgtacc tttaatttcc
      121 aattgatatt ttactcaatg gtttaatatt cttcaggtca gtataatcgt actgatagat
      181 accatcggct gctatcatca tcagagtttt ttcgtaagct atcagatcaa agccgtccat
      241 ccctttatcg tgtgccagca gattagccgg atccatgata gtgagcggat tagaggcatt
      301 aaacactttc aatccttcgt cgcagaggaa caacgtgccg ttgtcaattc ccagtccttt
      361 cgggtttttc atttcgtacg aaacgatgtg tttgggctgt ttcgggtcct tcacgttgta
      421 aataagcagc tgattggagt tctgaccgca gaaattaccg gagtgaatcg ttacgtaggc
      481 cacatcattt tcaaccacta ccggatcgca acccagaatg tgggtgattg aagccatccg
      541 ttcgggcttc agcgggtctg ccacggagta aatcagcatt ccggtaggtg tacccatata
      601 catgtagtcc ttgtagctga aaatggtttc cacattccat cccacgtaaa aatcctcgct
      661 tgatttcacc ggtgtcggat tttcgatgtt gaaaattccg agctgattat tgagtaccgt
      721 gtaaagatta tttttgtaaa tggcaaaacg cgacatggag cctgtttggc ctacagctcc
      781 ttttgcgccg ccggtgtaag cagagttagc catcggagca gaatcttcaa gcattccgcc
      841 gcgccaccac caccagtaac ttcgttcgcg catgtacttc tcggttcttt ccttcaccac
      901 ccagcctatc acaatgccgt tgttgcgatc catgcttttc tcatagtcgc atacgaaatt
      961 gttatcgatg gagggtactg atgttgggaa cacctcttcc ttgcggccaa gcaggcgggg
     1021 ctgagcaggc gatgagacat caaaccacac cagatcgatg tacgaatcgg catacaggat
     1081 attgtctttg atggccatat cggcatttcc gagcagttcg atgaagccaa tcacgttagg
     1141 tttggccgga ttgcggttgt caatgatgtg aattcccttt tcgggttcag aaatatacat
     1201 gtatccctgg aaaaaggcaa ttttgccctg cttgttgatt ttctgaggta catccgtcac
     1261 tctcaccgac ttgcgaaaca aatcggcaga cataaatacg ggctcgttga ctttgtaagt
     1321 gatggtttct tcgaccgatt ccgagcagcc tcccaacaga aaggtgccca gcaatagaaa
     1381 taaataattt atccgtttca tattattttg tttttgagag aattattttt gctttgtatg
     1441 aatcatattt caattttttt cccaccgaag gataaggatc aagatatata agcttaattt
     1501 tcagtccgtt gtatgttgcc gaatcagcga aatggctaaa tttcagcgtg taaatatcta
     1561 aggattttct tgattcggaa tcatcctcaa caaggaaacg aatgccggca acgccgctcc
     1621 agaagcaata cacaccttcg gggcatcggg aatcggtgaa taccgaatcg aatttcacga
     1681 tgtattttct gtcagtggtc cttacagtat ggttcaccgg aatcactgtt tcgcggtcgt
     1741 aggctgcggt gcgtatttca tcccatccca cgtcatttat gttttcgcat ccgaacgaaa
     1801 gcatacacag tacagtaatg acggacaaaa ctgacttttt tatgtgtaaa ttattattca
     1861 tttgttgttc cctgcaaatc cggaaaactc atctggaaat tttccctgat aatttatttc
     1921 tttaaaactt ttccgcattg cccactttcc ccataaacag aatgatgccc gaacttttct
     1981 cacggataac aaatgtaaag ggcttgttga caatgaagta cggatgctcc ggcactgaag
     2041 tattctttaa tccgattgtt gttactgctg ctgcttcggt tccttcttcg gtgacttcca
     2101 cataggtatc gtgctgtacg aatccgatgt agagcggttt caggatggat atattgtcaa
     2161 attcggcatt tggttgaaaa gctttgagca tgcccatctg ccggagcggc tctttcagct
     2221 ggaatctgtt tttcaccctg aaacggggca tcgaaacgat tactttctgt acatcgagtc
     2281 ctttcagcac ctgtgaatat ttttcaggag taagggtttt gaagacatcg gcggcccggc
     2341 cggttccttt cggcaggatt accgtcatgc tgaatgcctg gttgccgtaa ggcatgtcca
     2401 ggtactgcgc ataatcatct tcggtgtagg caaaggtatc ggttatgttc atcatattga
     2461 cctttacccg ctggttgagt tcgttggtga agtccgattc gcgggtgtct tttttgtcaa
     2521 actgctttac ccacgttccc ttgaagtaga tggcattaat cagaaacatc atttgagccg
     2581 gatcaattgt cttaatcact tcggggatga gtccgttggt atggtcgctg caccacttgt
     2641 tgatggtttt cacggcatcc gggctattga agtccagggc ggcaacttcc gagttgaaat
     2701 aatcggtgtt cgttttcaga aaaccggctt tcacgctgaa cgaatttctg taccagatgg
     2761 agttggctat ggtcagcctg gttttcggat cgagatcagg cagggtggtc tgcatcagtt
     2821 tgtagtactc gttgatttcg gcatctgtga gtccttccat tttcagagca gtttccatct
     2881 ccgttttagt gactcctata gcaccgtttc gggccatacc gagtgcaatg cttacactca
     2941 gcggagatat gaacacgttt tcatcagacg tatctgccat cgtccggttc atcaaatcaa
     3001 aggcaaattc attgtcctga gccacgcgct gtttctgcac agctgtcagg ttgatggggt
     3061 ctgccgtccc gtcaggcaga ggattggtgt tatcgcagct tatcagcagc gaaatgcaca
     3121 ccagcagaat aatcggattt ttcatttttg aaaaagatat tatcagtcga ttgctattaa
     3181 aaaagaaaca aatttcattt acttaaaatt ttcccacatt tcccagtttc cccatgaaga
     3241 gaataactcc cgaacttttc tcacggataa cgaaagcaaa gggcttgttg actataaagt
     3301 acggattaac gggcaaggat gtggtttcga aaccgattgt tgttactgct gctgcttcgg
     3361 ttccttcttc ggtcacttcc acgtaggtat cgtgctgtac gaatccgatg tagagcggtt
     3421 tcaggtcgga tatattgtca aattcggcat ttggctgaaa tgctttcagc atgcccatct
     3481 gccggagcgg ctccttcagc tggaatctgt ttttcaccct gaaacggggc atcgaaacga
     3541 ttactttctg tacatcgagt cctttcagca cctgtgaata tttttcagga gtaagggttt
     3601 tgaagacatc ggcggcccgg ccggttcctt tcggcaggat taccgtcatg ctgaatgcct
     3661 ggttgccgta aggcatgtcc aggtactgcg cataatcatc ttcggtgtag gcaaaggtat
     3721 cggttatgtt catcatattg acctttaccc gctggttgag ttcgttggtg aagtccgatt
     3781 cgcgggtgtc ttttttgtca aactgcttta cccacgttcc cttgaagtag atggcattaa
     3841 tcagaaacat catttgagcc ggatcaattg tcttaatcac ttcggggatg agtccgttgg
     3901 tatggtcgct gcaccacttg ttgatggttt tcacggcatc cgggctattg aagtccaggg
     3961 cggcaacttc cgagttgaaa taatcggtgt tcgttttcag aaaaccggct ttcacgctga
     4021 acgaatttct gtaccagatg gagttggcta tggtcagcct ggttttcgga tcgagatcag
     4081 gcagggtggt ctgcatcagt ttgtagtact cgttgatttc ggcatctgtg agtccttcca
     4141 ttttcagagc agtttccatc tccgttttag tgactcctat agcaccgttt cgggccatac
     4201 cgagtgcaat gcttacactc agcggagata tgaacacgtt ttcatcagac gtatctgcca
     4261 tcgtccggtt catcaaatca aaggcaaatt cattgtcctg agccacgcgc tgtttctgca
     4321 cagctgtcag gttgatcggg tctgccgtcc ggtcgggcac aggattggtg ttatcgcagc
     4381 ttatcagcaa cgataccaac aacagcatta aaacgggtgt tttcattttt ctaaaaattt
     4441 gaaggtatta caattctatt cttaatcctc cgttcagacc gatggtgagc ggctgctcgg
     4501 tgcgcgcgct tctcggttgg ttattccgga ggtaatacgt taattcaggt tctgcataca
     4561 ggctcagccc tttccggagt ttatattctg ctcccagcgc accgttgagc gaccattgca
     4621 gcccgtcaat tcccgatttt acattcgtat tggcggtagc ctgctgatct tctatttcct
     4681 gcatgtaaat ggatcgcagt cccttctcca ccattcctcc ggccgaaacg tagaggttcc
     4741 acttcggaaa atcaatcagc agtgctctca tattaagcgg aacacccagg tagtgcagtt
     4801 gcagtacgcc gcggtagttg gtcagtcccg gtcggtagta ttcggagcgg agataggtat
     4861 atatgaggcc gctttcgaga cttaaagtcc tgttcagggg ctttaccagc atgatccctg
     4921 ccgaaaccgg aggatcgtgt tttgcttccg tataatcgtt ggcatcgagc acggtggtgt
     4981 agttggaggc aatttctctt tttaccagac tcggactgct atcgtagaat tttccccctc
     5041 catcgagttg cgatatggat gacaaactgc ctcctgtacc cactgcagcg gctatgagca
     5101 acggtctttt tttcaatggc tggtgtttgg cgggcggtac ttccggatag tcgttgaggt
     5161 cgggcagcga agtgaccgga gttccggtgg ttgttttctt ttgccgaacc gaatcaacaa
     5221 cggtattgac tgcatcgttc atcacttttt cttccggttt ctgcacttcg gcagtctgag
     5281 tttcagttat tctggtttca ccgggagcgg tagtgtatgg cttgttcctg ttttcggtta
     5341 ttaaaagttg tttttctgat gttgatgctg tttgatctga taaattattt ccagtgtttt
     5401 cagtattttt attgcttccg gttagtgccg taaatacatc ttccaccttt tcagcagcct
     5461 ggatttgcct gtcatactgc aactgagggc ttgaatccgg tttcagcatt tcagtctgct
     5521 gtacgcctgt tagagccggt atttgttttg tttcataaaa aggattgatg atgagcaaaa
     5581 gtgcaacgac tgccactgca gcgcctgaaa tccagtacca caccggtgtg ataattcgcc
     5641 gccggggagt catcttttgc tctatggcat tccagatgct ttcgtccacc ggcaactgat
     5701 gattttcgag cttttccttt atcagggtgc tgaaaacatc tttttcgtct gtatgtttga
     5761 tattatctga cattatcttt tattcattaa atccgacacc agttgctgta atgcttttcg
     5821 ggcacgtgca aactgtgacc gggaggtgtt ttcctgaatg ttcagttttt cggctatttc
     5881 ggcatgcgag tatccttcta tagcatataa gttgaatatg gagcggcttc tgtcgggaag
     5941 ttcggctaca cattccagca gttcgtcggc tgaaagtttg tcaagtaccc gatggtcaaa
     6001 ctcatcgatc gaatccgaaa aatgatccat gctgacattc attttcaata tttcattttt
     6061 ctgaaggtat tcgagcgcgg tgtttacaaa aatctgacgc agccaggccg gtagcggtcc
     6121 acgtgcctca tactgaccga tatgggtaaa taccttcaca aatccatcct gtaaaacgtc
     6181 ttttgcagtt tcgcggttgg caacgtaacg tacgcacaac gaaagcatgg caggggcata
     6241 tttctcatac agttccctcc ttgcccacgg ctcgccccgt ttacagccgg ctattaaaag
     6301 ttgttcgttg tccgaaatca tcccttgttt attatatgat ggtatttgtg ttcaaaacgc
     6361 tgcaagagct ttttaaaatt aacattattt aagattgatc cggcagtgca ttgttttttt
     6421 ttgaaaagag ttatctgtcg agaaactaaa aaaagccaag ccatttgaca cgatgtctta
     6481 agcccggctc ttttataata agaaatttat accaattact actatttgct tccgtcagca
     6541 aggcgaagca gcacgcagct gagaattact tcagccactt atccagccat ccgaagaagg
     6601 tacgctgcca cagtacacca ttttgcggct gcagtaccca gtggttttca tcggggaaga
     6661 taagcatttc ggccggaacg cctctcagta ccgcagcatt gaaggcagcc atgccctgcg
     6721 aggcaaggat gcggtaatct ttttcaccgt gaatgaccag tatgggtgta tcccatttat
     6781 ccacaaagag atgcggcgag ttggcatacg accgctgagc agtggcattc tgtttatccc
     6841 agtacgcacc acccaaatcc cagttcacaa accacatttc ctccgtttcg aggtattgtt
     6901 gtggaaggtt aaacatgccg tcgtgagcta tgaatgcctt gaagcgtttc tggtgatgtc
     6961 cggcaagcca gtaaaccgag aaaccaccat agctggcacc cacacatccg agtttatctt
     7021 tattcacaaa aggttcctta gccaccgcat cgatggcaga gaggtaatct ttcatgtttt
     7081 gtccaccgta gtcgccgctg atctgtgtaa gccattcatg accgaatccg ggcaatcctc
     7141 ttctgttggg tgctacaaca atatagccgt tggcagccat gatctgcaga ttccaccggt
     7201 atgaccagaa ctgactcacg gccgactgag gtcctccctg gcagtaaagc aacgttggat
     7261 atttcttgtt tggatcaaaa tccggcggat ataccaccca ggtgagcatc tgttttccgt
     7321 cggtagtagc tatccaccgt tcttccacgt tgcccatctt cagtttttcc agtatttccc
     7381 tgttctcgaa ggagagttcc ttttcttcgc ccgtagtctg gttgatggca taaatttcat
     7441 ccggtttgct cagcgagtgt tttacgccga tcaatttgtc tttcagcgga ataaccgatt
     7501 cgtaatcgtg catgccccgg gttacctgag ttatggaatc ggtgagtaca tcggcgcggt
     7561 acacctgtgt agttccgtgc catacgctta caaagtagat gtatttgctg tcgggcgacc
     7621 agctcagtgc attcgaactc tgatcgaagc gagcactcag gtcttttttc tcccctgttt
     7681 tggtattcag tacatacaac cgacttttgt ccgattcaaa cccgtcgttt tccatgcttt
     7741 cccaggccat ccattcgccg tcgggtgaga aaaccggatt ctgatcgtat ccgggcatgc
     7801 cttcggtttt attttcggaa gttgcatttt cggtgttgta caggtagata tccgaattgg
     7861 tggaaagtgc atattcttta cccgtttttt tccggcaggt gtatgccacg gtttttccat
     7921 cgggactcca ggccagttgt tcgataccgc cgaaaggttt catcggactt tcatagggtt
     7981 cacctgccag aatgtcttta atattttcaa gcattccacc gacaaaatcg gccaggaaag
     8041 ggtgcggaac ggtttgtacc cattcatccc aatggcggta catgaggtcg ttgacaataa
     8101 ttccgctgga tttggggagg tcgggatgct tgtcaaccgt acgttctccg tatttcacat
     8161 ccgccacgta gagcagtttg ctttcatcgg gtgagaatgc aaaatcgttg attccgccct
     8221 ctgtttcggt gaggcgggta cgtcgcgaac cgtcggcttt catggtccag agttgcgagg
     8281 agcccgattc gttggagagg aaagcgatgg tttgactgtc gttcatccat ttggctgccg
     8341 attcgcggtg cggggtggcg gtaatttgct tccggtctgt tccgtccacg ttcatcacaa
     8401 agatttcgga gttgcttttg ttttgttcga cgctgtaata gcttacggtg tacagaatct
     8461 ttgtttcgtc gggcgatacc tgcactccgc caattcgtcc gaaagcccag agcacttcgg
     8521 gagtcagtaa tccgttttcg atgacgggag tttgtttgcc gatgagaggc gcatcgtcag
     8581 ttttcttttc attattgtcg cacgcagcaa gcgctgtcag tactaatgcc ataaaaattg
     8641 ttttaagttt cattggtgaa tatttgtata agtttcttta ttttcagcgg tcggtacaat
     8701 agcacgtttg atttgatgca ggcaaatctt ttccactatc cggctgcaaa tatacaatat
     8761 tattttttta tacgggaaaa tcgatttagc gaaagaactt ttatagttgt ttggatgttt
     8821 ggttgtttgg ttgttgaaat tcaccaattg taatttgtat attaataaac tgtaaataaa
     8881 catgattgat tcatttgctg accgggctgc ctattgggat caacccgaca aagaaaaaat
     8941 ggctgaaagg ttcgttaccg aacttcaccg ccgtgtaacc ctgcagggca gctggaaagc
     9001 gctcgaaata ggagccggaa cgggtctggt gggtttaatg ttgctgcctg acctggcttc
     9061 aattgttttt gaagatacat cagcctcaat gctcgatgtg ctgcgaaaga aaaccatcgg
     9121 gatgagtaat attgacatct tacacgggga gataacagac tacaacaggc aggatatcga
     9181 cctggtaatc tccaacatgg cttttcacca catcgaagac attgctcaca ccctgaaaca
     9241 cctgcacagc ataacaagac ccggcgctgt ggtggttatc ggcgatatac gttccgagga
     9301 cggatcgttt caccgctttc agcccattcc ccaccgcgga tttgataccg gtgcgctgtc
     9361 ggtgctgttc gaacaggcag gatttgcagt agatgcagtg gaaacttacc acgtgctgcg
     9421 gcaggaaaaa attcccggaa caatttcgga atacgagcaa tttatactgg tggctcacaa
     9481 atctgcgtag agcggtgttg tgcccgttct tttgtccgtt cagaccggaa aaaaggttaa
     9541 aaacagcaat aattgatttt tttttaaaaa aaatcgatta aattaaccaa aatccttgca
     9601 gggacgataa aagattgtat ttttgtaaaa aaattatttc cgttttatat aaccacataa
     9661 attttaaatg ttttatgaaa gaacttgtag caaaaattaa cgcagaaatc gctgctttcg
     9721 cagaaaacgc agctcttcag gcagacaaag gtaacaaagc agcaggtgca cgtgcacgta
     9781 aagcttctct ggatttggaa aaatcattga aagaattccg taaagtttct atcgcagctt
     9841 ctaaataagt taccacatag agcttttctt tcagcgaaca aaaccattgt tccgtaaaaa
     9901 aattaagaaa aggctttaat cgttaagatt aaagcctttt cattttccaa gttccacgtt
     9961 tcacgttctg ttgcggaata tccggattat tatgatggtt ccggcgacta ttaacaataa
    10021 ctgctaatac aaaaccggct tactgctgtc taaagaatcc gttttttctt tttattatct
    10081 ttattatagc catatccgta gctgctatag ctgccgtagg taccgtagcc ggagtggaaa
    10141 ttggaaacat cgttgaccac gagccccata ttcggaagca ttttcttatc ggacaagtcg
    10201 ttagcaaaac ggaggttgtt cttgcgcgag tagttggcgc ggcatacgta catggtggca
    10261 tcggcaacgc gtgaagccac gatggtatca gtaaccagac tcaccggagc ggagtcgatg
    10321 atgatgtaat cgaatttctg cctgagcact tccatgcctt tttcgaactc ggtacgggca
    10381 atgagctcgg cgggatttgg cggaatcgct ccggccgata tcaccgacag gttgggattg
    10441 atgcccgagg gctgcacaat atccagaata ttgttttcgt tgccggaaag gaattgcgac
    10501 atgccgtgtc cggtatcaat gttgaggtat tccgacaggc gcggcaagcg aaggtccatc
    10561 cccatgagca gcactttttt gtcgagcagc gagaggctga tggcggtatt gatggcgatg
    10621 aatgtttttc cctctttcgg ttcggtggaa gtcaccacca ctaccttctt atccttgccg
    10681 agtgtaaaga gcaggttggt acgaagcatc cggaaagctt cttctgtttc gcggttctcg
    10741 ttggcaacca cggctatatt tcccgaagcg gaaaccggaa tttcgcccag cgaaggaatg
    10801 ttggagtatc tgtcgatgtc ggcgcggctc tcgatacgga agtgaagcag gtctttcagg
    10861 aagatgatga gtgccggaat gaggagtccg aggaagaggg caatcataag caccatcatc
    10921 cgttttggtt tgatcatcct acctacggta gcttcatcga gtactttggc tttgggagcc
    10981 gtagcagcca gcgagagggc gttttcttcg cgtttctgca gcagcataag gaaaaggctc
    11041 gatttgattt gctgttcgcg ggctatttca gtaaactgac gttcctgagt aggagctgcg
    11101 ccggtctggc ttttgtaata gttcacctgg ctctgcgcat cgcggcgtgc tatgctgagt
    11161 ccctgtttca cactggcaat gctactttct attcctgccc gaaggccttc tatctgaccg
    11221 tcgagtttct gaatcaccgg attggactcg gtgttggtac gcagcagtcg gtcgcgttcc
    11281 aatatctgcc ggttgtattc gttgatggtg gcagccaggg tcggatcttc tatccccaca
    11341 ttgctcggaa tcagcttgtt tctgttccgc tgttcgttga tgtaggtatc caggtaatcc
    11401 accagattga gctgagtgct tacttccacc agtttctgct cgtactgact ccctttttgc
    11461 agtgacatct gtacatccga agtaatatcg gtcagcccct gacttcgctt gtaatcttcc
    11521 acctgtcttt cggcacttcc gagttcattg ttgataatag ccagacggcc gtcgataaat
    11581 tcctgtgtat tcagcgcttc cttgttctta tcagcagtag catccatatt gtacaccccg
    11641 acgagtgcat tcaggaaatc gattcccttc tgcgtttcgg tagtagtgta agccaaatta
    11701 agcaccgatg tggttttgga tgtgggcgcc accgcaagtc ccgaacggaa atagggcacc
    11761 atcgacatgg gttttcggat tacgacctga atcggcacgc cgtagttggg tgctttgccg
    11821 gcacggaacg aaacggtgag aatgccggcc ggagttttgt agagtgcggg catttgtttg
    11881 attttggtgt tggtcttttt gccgttgaag gtagccgtta tatccacact ctggtccttg
    11941 ttgataacgg cagtgagcat taccggactt ttg
//
LOCUS       DAORKP010000065        11749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_567_length_11749_cov_9.756713, whole genome shotgun sequence.
ACCESSION   DAORKP010000065 DAORKP010000000
VERSION     DAORKP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_11749_cov_9.756713"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..75
                     /locus_tag="PLN75_05100"
     tRNA            2..75
                     /locus_tag="PLN75_05100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Arg,seq:acg)
     gene            344..727
                     /locus_tag="PLN75_05105"
     CDS             344..727
                     /locus_tag="PLN75_05105"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRG03529.1"
                     /translation="MKTVILTSCNNAVEASLIQGNLANEGIESFISNENYTSLYPNMN
                     GAMGSGIQIFVNEEDAERALIVINKSDQSIHCLNCHSKNVKLKGGKNILKVVGIMIMS
                     LFSSKASGNSKSKYHCNDCGSDFEV"
     gene            complement(840..2546)
                     /locus_tag="PLN75_05110"
     CDS             complement(840..2546)
                     /locus_tag="PLN75_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRG03530.1"
                     /translation="MMNFYTDNPALKFQLSHPLMQKIVSLRERNFSEKEKYDFAPQDF
                     EDAIDNYDKVLEIIGEISGETIAANAESVDKEGPQVISSRVKYARGTQENHEALVQAG
                     VYGMSLPREFGGLNFAMTPYVMSGEIVSRGDAGFANIWGLQDCAETICEFGDQDVKAE
                     FLPRVTLGETCSMDLTEPDAGSDLQAVQLKATEKDGKWYLNGVKRFITNGDAQIKLVL
                     ARSEEGTQDARGLSYFVADNQFDHTIKVRRIENKLGIKGSPTCELVFTNTPAKLIGTR
                     RMGLIKYVMSLMNGARLGVGAQSVGVSEAAYQEAINYANDRKQFGKAIIEFPALFEML
                     SVMKAKLDATRALLYETARFVDLYKAYEGIEQERKLTPEEKAEYKEYMRLADAFTPVL
                     KMFASEFANQNAYDSIQVHGGSGFMKDYACERIYRDARILTIYEGTSQLQVVAAIRYV
                     TTGAYLKKIREYEQMSIKPEFQNMRKRLMSMTNLLETAVARVDELKNQDYLDFQARRL
                     VEMAGHIIMCYLLILDASRDDKDMFRKSAEVYLNYAEVEVRKNATFIKQFNPENLDTY
                     RM"
     gene            complement(2712..3731)
                     /locus_tag="PLN75_05115"
     CDS             complement(2712..3731)
                     /locus_tag="PLN75_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRG03531.1"
                     /translation="MNNLIVYLEIEDGIVSDVSLELLTKGRSLADQLKCRLEAVAIGY
                     QLDKIAEQVFPYGVDVLYLADDKRLSPYTTLPHTSVLVNLFKEEKPQIAFMGATSVGR
                     DLGPRVSSALYSGLTADCTALEIGTYEDKKAGKKYENLLYQIRPAFGGNIVATIVNPD
                     CRPQMATVREGVMKKEIRDPKYKGTVKKLDVSKYVSDEDFVIEVIERHMEKSKLNIKG
                     SPIIVAGGYGMGSAENFKLLYDLADTLGGEVGASRAAVDAGFAEHERQIGQTGVTVRP
                     KLYIACGISGQIQHIAGMQESSMIIAINNDPYAPINAIADYVITGNVEEVVPKMIKYY
                     KKNSK"
     gene            complement(3843..4715)
                     /locus_tag="PLN75_05120"
     CDS             complement(3843..4715)
                     /locus_tag="PLN75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRG03532.1"
                     /translation="MGFNIIVLAKQVPDTHNVGKDAMKADGTVNRAALAAIFNPEDLN
                     ALEQALRIKDKNPGTTVSLLSMGPVRAAEVLREGLYRGADKGYLLSGREFAGSDSLAT
                     SYALSCAIRKMGKVDMIICGRQAIDGDTAQTGPQVAEKLGFPQITYAEEILDVSEKTI
                     TVKRRLERGVEVLRGQLPIVVTVNGSAAPCRPRNAKRLMKYKYAKTQSERQESGTDYT
                     EFYDNHSYLNITEWGASDVEHDPKWLGLSGSPTKVKAIESVVFTAKESKKLTADDEQI
                     EEMMKELIANHTIG"
     gene            5295..6017
                     /locus_tag="PLN75_05125"
     CDS             5295..6017
                     /locus_tag="PLN75_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HRG03533.1"
                     /translation="MKKIVFMFLALYLMNTVNGQNFQVHYDFGKNRNYVTTTFEMFKP
                     DNWGNSFIFVDFDYTMDKAKHPGMAYMEIARCFSFGKSPFSFQVEYNGGLMAIPAETG
                     YSAFAINNAYLSGLDYGWHSADFTKFLNLKVLYKYIAGKNPASFQLTGVWNLDFFNRK
                     LSMNGFADFWREDNLNYTKPNGENLSEPTETRFVFMSEPQIWYNLTPQLSLGSEIELT
                     SNFGSVDGFKVCPTIGIKWTFQ"
     gene            6150..7121
                     /locus_tag="PLN75_05130"
     CDS             6150..7121
                     /locus_tag="PLN75_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRG03534.1"
                     /translation="MSLLLMLSTCTKTPGKSQNEQPENIKTVSVPDFNADSAFLYIKK
                     QVDFGPRVPNTHQHVACGNYLKQKLTDFGAQLTVQEADLEAFDGTILKASNIIGSYNP
                     EAATRILLFAHWDTRPWSDHDPDSKNHSTPVSGANDGASGVGVLLEMARHFGKEKPAV
                     GVDIIFFDAEDYGAPADKMSPENENSWCLGSQYWASNKHIPGYRAKYGILLDMVGAPQ
                     ARFYREQISEYYASYVVDLVWEKAASLGYAHYFINQPGGSITDDHLYINQKAGIPSID
                     IIHYDPNTATGFGHYWHTVNDTMENIDKNTLSAVGKTLMQVVYNEQP"
     gene            7128..8210
                     /gene="proB"
                     /locus_tag="PLN75_05135"
     CDS             7128..8210
                     /gene="proB"
                     /locus_tag="PLN75_05135"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRG03535.1"
                     /translation="MELRYKNIAVKVGSNVLTRSDGTLDVTRMSAITDQIAYLHKLGV
                     KIILISSGAVASGRSILKSSKKLDVVEQRQLFSTVGQAKLINHYWELFREHNMVVGQV
                     LTTKTNFEGRNQYLNQRNCIRVMLENGVVPIVNENDAVSVSELMFTDNDELSGMVASM
                     MGMEALIILSNIDGIYNGSPAEETSEVISEIKKGQYLSDFIQHSKSTFGRGGMQTKVT
                     IARKIADEGIEVYIANGRKENILIDLLNRKKNVLSTHFIAEKTGVSSIKKWIAHSDGF
                     AKGEIIINENAARALTGTNAVSLLPVGIINISGRFEKDDIVKICDEKGKYIGVGKVRY
                     SSEKATLLIGKKNQKPLIHYDYLHLE"
     gene            complement(8217..8666)
                     /locus_tag="PLN75_05140"
     CDS             complement(8217..8666)
                     /locus_tag="PLN75_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03536.1"
                     /translation="MAKGRRKGCLFLVILTGIFVGAYLTNPTEDMHREAAKEKLNKMA
                     LNLLSKYGIEESFVAGLGIDLSGKYVDEFIQNHVSADNYYLFSTTRVHWSGESRIIGI
                     GAFGRVYISGKVDEILERELENFVKEKIEGIKIPGFDLKDFKLELDL"
     gene            complement(8674..9435)
                     /locus_tag="PLN75_05145"
     CDS             complement(8674..9435)
                     /locus_tag="PLN75_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRG03537.1"
                     /translation="MLSKSKIKWINSLERKKNRDKENVFVAEGVKIVTDLLPFFKCRF
                     LAVTQSEVSDKFSKYTDEIEVISQDEYRKITFQKSPQGVLAVLEMPEYKIDYPKLTMN
                     LSLALEEIQDPGNLGTIIRIADWFGIKDIFCSQHTADVFSPKSIQATMGAIARVRIHY
                     VDLALFLDKIKDKIPVYGTYMNGENIYDLTLSDSGIIIMGNEGNGISAEIEKLITRKI
                     AVPTFPEDSIASESLNVATATAIVAAEFRRRTHSK"
     gene            9595..>11749
                     /locus_tag="PLN75_05150"
     CDS             9595..>11749
                     /locus_tag="PLN75_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRG03538.1"
                     /translation="MLLFAAACNTTRYVPENEFLLDKVSISSDNKTITKEETKSYIRQ
                     LPNAEVLGLFKMQLGLYNISGPDSTKRWNRFWKRVGDEPVIYNPQLTSVTENQIGQLF
                     RNRGYINAEVKSKVNYPKEKIAEVTYQITSNRPYKIRGYYANINHPELLEIASDTARS
                     LIKPGNLFDSDVLDDERDRVTSRFRNLGYFNFVKNYLQYYADSTLNSHEVDLVLEVGN
                     EANKNDSAFLKKAFQKYMIRKVNFYSETDKTIENLNSKILRDSLEIENYSFVYENNRN
                     IRPSVLIQSTHIVPDEFYSDRAVERTYSSLNSLSAIKYMDISFKEVNSNQLDANIILT
                     PNKVQSLSTDLEGTYSAGYWGLGGNINYGHRNIFRGSETLSLRGRAAYEYQGKDQNAY
                     ELGGDIGLKFPTFLLPFATRDIKRNVRATTEINGSYSYRNRPKEYTGIVTGIGFKYNW
                     SERFEIKHNVDVLNISYVYYPFISPEYRAYLSTSPYFVYNFQNHLIMRTGYSGSYSSF
                     RPLQPLRNYSTLNYGIETAGNLLYGMNNLLNSKKDTAGTFKIFGIRYAQFVKTDFNVT
                     RHQIFDKNNRIVYHAGIGVALPYGNNNLIPFEKRYFSGGANSVRGWTAYQLGPGTYKS
                     KGGYIDYNTQMGDVRIDLNMEYRTKLFWKLDGALFLDAGNVWTIREYESQPGGVFRFS
                     DFIGQMGVAYGGGLRADFSFFVLRLDLGLKLHNPAL"
BASE COUNT         3454 a         2473 c         2389 g         3433 t
ORIGIN      
        1 tggccccgtg gctcaactga atagagtatc tgactacgga tcagaaggtt acaggtttga
       61 atcctgtcgg ggtcacttaa ataaaaccta aataactatt atacagttat ttaggttttt
      121 tgttttatct gcttgcataa tttttacata catttacata cattctgaca acgacaattt
      181 atccttacca ttctttcgga aactaattca aacaaataaa atcccaacct ttcagaatct
      241 gatttaatat tcatttcaat agaattttcc acgaaaaaaa cttttctgat gctttttcaa
      301 ctgataattt ctatatttgt gtaaatattt aaccgttaca gccatgaaaa ccgtaatcct
      361 tacatcgtgc aacaatgctg tggaagccag tctgatacag ggtaatctgg caaatgaagg
      421 cattgaaagt ttcatttcca acgagaacta cacatcgctt tatccgaata tgaacggagc
      481 aatgggaagc ggcattcaga tttttgtaaa cgaggaagat gctgaaaggg cgctgattgt
      541 gatcaacaag agcgaccaaa gcatacactg cctgaattgc cattcgaaga atgtaaaact
      601 taagggtgga aaaaacatcc tgaaagttgt cggaataatg ataatgagct tattttcctc
      661 caaagcctcc ggaaatagca aatcaaaata tcactgtaac gattgcggaa gtgattttga
      721 agtataaaat ctcctgattc tgcacagaac ttatcgaaag aaaaccgctg aaaattaact
      781 aattttcagc ggtttattta tctgtgtaaa tctgtctaat ctgtgttatc agtgttctgt
      841 tacattctat atgtatccag attctcggga ttgaactgtt tgataaacgt ggcgttttta
      901 cgaacttcca cttcggcgta attcagatac acttcggccg atttgcggaa catgtcttta
      961 tcatcgcggc tggcatccag aatcagcaga taacacatga tgatgtgtcc ggccatttct
     1021 acaaggcggc gagcctgaaa gtccagataa tcctggtttt tcagttcatc aacacgggct
     1081 acagcagttt cgagcagatt agtcatgctc atcaggcgct tacgcatatt ctgaaattcc
     1141 ggcttaatcg acatttgctc gtattcacga atttttttca ggtaagctcc ggtagttaca
     1201 tagcgtatag cagccaccac ctgcaactgt gaagtacctt cgtaaatagt caggatacgt
     1261 gcatcacggt aaatgcgttc gcaggcataa tctttcataa aacctgaacc tccgtgaacc
     1321 tgaatactgt cataagcatt ctggttagca aattcactgg caaacatttt cagaacggga
     1381 gtaaaagcat ctgccagacg catatattct ttgtattcgg ctttttcttc tggagtaagt
     1441 tttctttcct gctctattcc ttcatacgcc ttgtacaaat ctacaaaacg ggcggtttca
     1501 tataacaatg cccgagtagc atcgagtttt gctttcatca cagacagcat ttcgaatagt
     1561 gccggaaatt cgatgatggc tttaccgaat tgcttacggt cattggcata gttaatggct
     1621 tcctggtagg cagcttctga tacgccgaca gattgagcac caacacccaa gcgtgcacca
     1681 ttcatcaacg acatcacgta tttaatcaat cccatacgcc gtgtacctat taattttgcc
     1741 ggtgtgttgg taaatacaag ttcgcaggtg ggagagcctt tgatacccag tttgttttcg
     1801 atacggcgta ctttgatggt gtggtcaaac tgattgtcgg ccacaaaata cgataatcca
     1861 cgggcatcct gcgtgccttc ttccgaacgg gcaagtacga gttttatctg cgcatctccg
     1921 ttggtgatga aacgttttac tccgttcagg taccatttcc cgtctttttc ggtagctttg
     1981 agttgcacgg cctggaggtc agaccctgca tcaggctctg tgagatccat ggagcaggtt
     2041 tcacccaatg ttacgcgagg caggaattca gctttcacat cctggtcgcc aaattcgcaa
     2101 atggtttctg cacagtcctg caagccccag atattggcaa agcctgcatc accgcgagaa
     2161 acaatttcgc cgctcatgac gtaaggtgtc atggcgaaat tcagtccgcc aaattcacgt
     2221 ggcaacgaca tgccgtaaac gcctgcctga acaagtgctt cgtgattttc ctgcgtgcct
     2281 ctggcgtatt tcacgcggct gctgattacc tgcggtcctt ccttatccac actttcggca
     2341 ttggcagcaa tagtttcgcc gcttatttcg ccgatgattt cgagtacttt gtcgtagttg
     2401 tcaatcgcat cttcaaaatc ctgaggtgcg aagtcgtatt tttctttttc cgaaaaattt
     2461 cgttcacgaa gtgaaacgat tttctgcatc aacggatgcg aaagctgaaa tttcagcgcc
     2521 ggattgtctg tgtaaaagtt cataatatgt aagccccctc aatcccccaa aggggactta
     2581 atgagcagaa attagttgga tttatttaat ttcaaattat tcaatttcta ttataaaaaa
     2641 aactttgttt tcacctatct aacaaaagtc tccccttcgg tgtcccgata gctatcggga
     2701 tagaggggct tttacttgct attcttttta taatatttta tcattttggg caccacttct
     2761 tccacgttgc cggtgattac gtaatcggct atggcattga ttggagcgta cggatcgttg
     2821 ttgatggcaa taatcatgga gctttcctgc atgccggcaa tgtgctgaat ctgtccggaa
     2881 attccacagg cgatgtacaa cttgggacga acagtaacac cagtttgtcc aatctgccgc
     2941 tcatgctctg caaaacctgc atccactgct gctcgcgaag cacctacttc gccacccagc
     3001 gtatccgcca ggtcgtaaag aagtttgaaa ttttcggcac ttcccattcc atagccgccc
     3061 gccacgatga tgggcgatcc tttgatgttc agcttcgatt tttccatgtg gcgttcgatc
     3121 acttcaatca caaaatcttc atcggaaacg tattttgaaa cgtccagttt ctttactgtt
     3181 cccttgtact tcggatcacg gatttctttt ttcatcacac cttcgcgcac ggtggccatc
     3241 tgcggacggc aatccgggtt tacgatagtt gccacgatgt ttccaccgaa tgccggcctg
     3301 atctgataaa gcagattttc gtattttttt ccggcttttt tgtcttcgta agttccgatt
     3361 tcgagggcag tacagtcggc agtaagccca ctgtagagag ctgatgaaac tcgcggaccc
     3421 aagtcacgac ccaccgaggt ggcgcccata aaggcgattt ggggcttttc ttctttgaaa
     3481 agatttacca gaacggatgt atgtggcagt gtggtgtagg gtgacaaacg tttatcatca
     3541 gccagataca gcacatctac tccgtatgga aatacctgtt cggcaatttt atccagttga
     3601 taaccgattg ctacggcttc cagtctgcac ttcaactggt cagccagtga acgacctttg
     3661 gtaagaagtt ccagactcac atcacttacg atgccgtctt caatttcaag atatactatt
     3721 aaattattca tattcttttt aatttgccaa tttattaata tgccaaagtg ccaatataaa
     3781 tgctaattgt tctattgcca actaatacca ttggcatatt ggcacatttt ttcatttgca
     3841 tactagccta ttgtatggtt agcaattagt tctttcatca tttcttcaat ctgttcatca
     3901 tcggctgtga gctttttgct ttccttggca gtaaacacca cactctcaat cgcttttact
     3961 ttggtaggtg aaccagagag gccgagccat ttcggatcgt gttccacatc gctggctccc
     4021 cattctgtaa tattcagata ggagtgattg tcgtaaaatt cggtataatc cgtaccgctt
     4081 tcctgacgtt ctgactgggt cttggcgtat ttgtatttca tcaacctttt agcattgcgc
     4141 gggcggcagg gtgcggccga tccgttgacg gtaacaacaa tgggcagctg accgcggagc
     4201 acttctacac cacgttctaa acggcgtttt acggtaatag ttttctcaga tacgtcaaga
     4261 atttcttcag cataggttat ctgcggaaaa ccgagtttct cagccacctg cggaccggtt
     4321 tgagccgtat ccccatcaat agcctgacgt ccgcaaataa tcatatccac ttttcccatt
     4381 tttcgaatgg cacatgaaag tgcgtaagat gttgccagac tgtcggatcc ggcaaattcg
     4441 cgtccgctaa gtaaataacc cttgtcggct ccgcggtaaa gtccttcgcg cagaacctcg
     4501 gcagcccgca ccggacccat cgagagaagg cttactgtgg tacccggatt tttgtctttg
     4561 atacgaagcg cctgctccaa cgcattgagg tcttccggat tgaagatagc agcaagtgca
     4621 gcacgattga ctgtgccatc agccttcatc gcgtctttcc caacattgtg tgtgtcgggt
     4681 acttgcttgg caagaacaat gatattaaaa cccatattct tttgttttat aaatagatat
     4741 aatgtttgtc tgaaaagtaa tagtttttct gaaaaacgaa gacaaaatta ctgatttata
     4801 ttgaatttct gaaaattgac tgcattttta atagaattac agaaatttac gttaatttac
     4861 acatttcttt tcaaatctat ttttttataa attactcaac acgttttaac ggttttcaac
     4921 catttaaaaa ttaaaaaagg gcttcttgcg aagtcctttt ttattaggta ttagtaagta
     4981 ataaggtgta aaaagattgt gtttgttgta gaacgatgca aagtaaacac atatttcttt
     5041 acttaacaaa ttaaaaaata tgttttacaa cctatttttg tacttttttt gtaaatattt
     5101 tcttaaaaac atcatttttc tttgaaattc acaaatatca gtcatatatt actagaaaac
     5161 gaacatgtaa aacgataaaa tgttcagatt tttatcataa tgtcattgaa gagtaaggat
     5221 aaaataaaaa gatttgtttt gtgaacttat ttattttgaa atacctttgc aggactaaaa
     5281 aagaagatga aaaaatgaaa aaaattgtat ttatgtttct tgctttgtat ttaatgaata
     5341 ctgtaaatgg tcaaaatttc caggttcatt atgactttgg caaaaatcga aattacgtta
     5401 ccacaacatt tgagatgttc aaaccggaca attggggaaa ttccttcatt tttgtggatt
     5461 ttgattatac gatggacaaa gccaaacatc cgggaatggc atacatggaa attgcccgct
     5521 gcttttcatt cggcaaaagc cctttcagtt ttcaggttga atacaatgga ggattaatgg
     5581 ctattccggc tgaaacggga tattccgcct ttgctatcaa caatgcttat ttaagtggtc
     5641 tggattatgg ttggcacagt gcagatttta ccaaatttct caatctaaaa gtcttatata
     5701 aatacattgc aggcaaaaat ccggcttcgt tccagctaac aggagtctgg aacctggact
     5761 ttttcaatag aaaactatca atgaatggtt ttgccgattt ctggcgggaa gacaacctta
     5821 attatacgaa acctaacggt gaaaaccttt cagagcctac tgagaccaga tttgttttta
     5881 tgtctgagcc acaaatatgg tataatctca ctccacagct atcgcttggt tctgaaattg
     5941 agcttacttc taattttgga tcggtggatg gattcaaagt ttgcccaaca atagggatta
     6001 aatggacttt tcagtaaaaa ataacacaaa tcaacgctga cacgggatat atttttcgaa
     6061 catatatttt tagaaaatgt agtatctttg ctgaataaag tcattttaaa taaaaaaacc
     6121 gtaaatagaa aatgcgcatc ttactgtata tgagcttact gttgatgcta tcaacctgta
     6181 ctaagactcc cggaaaatct caaaacgaac aaccggaaaa cataaaaacc gtttctgtac
     6241 cggatttcaa tgccgactct gccttcctgt atatcaaaaa acaggttgat tttggtccac
     6301 gtgtgccgaa tactcatcag catgtggctt gcggcaatta tctgaaacaa aagcttactg
     6361 attttggagc gcagttaact gttcaggaag cagacctgga agcatttgac ggtacaatac
     6421 tgaaagccag taacattatc ggcagctaca atccggaggc agcaacacgc attttgcttt
     6481 ttgctcactg ggatacccga ccgtggtctg accacgaccc cgacagtaaa aatcattcca
     6541 cacccgtatc aggagctaac gatggagcaa gcggtgtggg cgttttactt gaaatggcac
     6601 gtcatttcgg taaggaaaaa ccggctgtag gagttgatat tattttcttt gatgccgaag
     6661 actacggcgc accggcagat aaaatgtcgc ctgagaatga aaattcctgg tgtcttggaa
     6721 gtcagtattg ggcatcaaac aagcatattc cgggctacag agccaaatac ggaattttgc
     6781 ttgatatggt aggagcacct caggcaagat tttacagaga acaaatatcg gagtactacg
     6841 ccagctatgt ggtcgatttg gtctgggaaa aagctgcctc gctcggttat gctcattatt
     6901 tcatcaatca gcccggtgga agcattactg acgatcacct ttatatcaat caaaaagccg
     6961 gaatcccgtc cattgacatc attcattacg atccgaatac cgctaccggt ttcggacatt
     7021 actggcacac agtgaatgat acaatggaaa atattgataa aaacaccctg tcggccgtag
     7081 gtaaaacatt gatgcaggtg gtttacaacg aacaaccctg agacccgatg gaattgcgat
     7141 ataaaaatat agccgtaaaa gttggcagca atgtgctgac acgctccgac ggcacactgg
     7201 atgtaacacg catgtcggca attaccgacc agattgctta ccttcacaaa cttggtgtaa
     7261 aaatcatttt aatatcatcc ggtgcggtcg cttccggccg aagcatacta aaatcatcca
     7321 aaaaactgga tgtggtagag cagcgtcagc ttttttcgac agtgggtcag gcaaaactta
     7381 tcaaccacta ctgggaactc ttcagagaac acaacatggt cgtcgggcag gttctgacca
     7441 caaaaacaaa tttcgaaggc cggaatcagt acctcaacca gcgaaattgt attcgggtga
     7501 tgctcgaaaa cggcgttgtg ccgattgtga atgaaaacga cgctgtgtcg gtttccgaac
     7561 tcatgtttac cgataacgat gaactctcgg gtatggtagc ttccatgatg ggcatggaag
     7621 cgctgattat tctgagtaat attgacggaa tttacaatgg ttcacccgct gaagaaactt
     7681 cggaagtgat ctccgaaata aaaaaaggtc agtatttgtc ggactttatt caacattcaa
     7741 aatccacttt cgggcggggt ggtatgcaga ccaaagtaac cattgcccga aaaattgccg
     7801 atgaaggtat tgaagtttac attgccaacg gcagaaaaga aaatatcctg attgatctgc
     7861 tgaacaggaa gaaaaacgtg ctttcgactc attttattgc cgaaaagacg ggagtttcga
     7921 gcatcaagaa atggattgct cacagcgatg gatttgcgaa gggagaaatt atcatcaatg
     7981 aaaatgcagc cagagcgctt accggtacaa atgcagtaag tcttttacct gtgggaatta
     8041 tcaacatttc gggcaggttt gaaaaagatg acattgtaaa aatatgcgat gaaaagggaa
     8101 aatatatcgg cgtcggcaaa gttcgttaca gcagtgagaa agcgactctg cttataggaa
     8161 agaaaaacca gaaaccgctt attcactatg attatttgca tttggaatag aatacattac
     8221 aaatccagct ccaacttaaa atccttcaaa tcaaatccgg gaattttgat tccttcaatt
     8281 ttttctttca caaaattttc gagctcgcgt tcgaggattt catccacttt tccggaaata
     8341 tataccctgc cgaaagcacc gataccaata atccggcttt caccactcca gtgaactctg
     8401 gttgtggaga acaggtaata gttatccgct gatacgtggt tttgaatgaa ctcatccaca
     8461 tattttccgg ataaatcgat tcccaatccg gcaacaaaac tttcttcaat tccatatttg
     8521 gataataaat taagtgccat tttgttgagt ttttcttttg cagcttcgcg gtgcatatcc
     8581 tcggtgggat tggtaaggta agcaccaaca aatattcctg ttaaaattac gagaaacaga
     8641 caaccttttc ttcttccttt tgccatatta tgattatttg gaatgcgttc ttcttctgaa
     8701 ttcagcagcc acaatggctg tagcggtagc tacattgagt gattctgaag caatgctgtc
     8761 ctccggaaag gtcggaacag ctatttttcg ggttattaat ttttctattt ccgcagaaat
     8821 accgtttcct tcgttaccca tgattataat tccgctatca ctcagagtaa ggtcgtagat
     8881 attttcacca ttcatataag ttccgtaaac cggtattttg tcttttattt tatcaagaaa
     8941 aagcgccaga tcaacataat gaatacgaac ccgggctatg gctcccatgg tggcctgaat
     9001 agatttaggg ctgaaaacat ctgcggtgtg ttgcgaacaa aatatatctt ttatcccaaa
     9061 ccagtcggct atacgaataa ttgttcccag attgcccggg tcctgaattt cttccagtgc
     9121 cagcgaaaga ttcatggtta acttcgggta atctatttta tattcaggca tttcaagaac
     9181 agccaaaacg ccctggggtg atttctgaaa agtgattttt cgatattcat cctgagaaat
     9241 tacctcaatt tcatcggtgt atttactgaa tttatccgac acttcggact gggtgacagc
     9301 taaaaaacga catttgaaga atggcagtaa atcagtaaca atttttacac cttcggccac
     9361 aaacacattc tctttatccc ggtttttttt tcgttccaaa gaattaatcc attttatttt
     9421 tgattttgaa agcatagaga agtattattt aattcattgg atgatttcaa ttgtttttta
     9481 cgttacaaaa ctaacaaaga aaacgagaat aaaacgtata tttgcaaaga ttttgaagaa
     9541 tttttgtttc aaacagctat gagaaacctg ggctattcat tcatactact tctgatgtta
     9601 ttatttgccg cggcatgtaa taccaccagg tatgtccctg aaaatgaatt tctgctggat
     9661 aaagtcagca tttcgtctga taataaaaca attacaaaag aagaaacaaa atcgtatatc
     9721 cggcagctcc caaatgcgga agtactcggg cttttcaaaa tgcaactggg actttacaac
     9781 atttcagggc ctgattctac caaaaggtgg aaccgcttct ggaaaagagt aggcgacgaa
     9841 ccggtgatat ataatccgca attgacctcc gttacggaga atcaaatcgg gcagcttttt
     9901 cggaaccgcg gatacataaa cgccgaagta aaaagcaaag taaactatcc gaaagaaaag
     9961 attgccgaag taacttacca aatcacatcg aacagacctt acaaaattcg ggggtattat
    10021 gccaatatca atcaccccga actgctcgaa attgcttcgg acacagcacg aagtttgata
    10081 aaaccgggga atctttttga cagcgacgtg ctggacgatg aacgtgaccg cgtgacaagc
    10141 aggtttcgga atctgggtta tttcaatttt gtgaaaaatt atctgcaata ttatgcggac
    10201 agcacactga attcgcatga agtagatttg gtactcgaag tgggtaatga ggcgaataaa
    10261 aacgactcag cattcctgaa aaaagcattt cagaaataca tgattcgaaa agtaaatttt
    10321 tacagtgaaa ccgacaaaac aattgaaaat cttaactcaa agatattgag ggattcactt
    10381 gaaattgaaa attatagctt tgtctatgaa aataacagga atattcgtcc ttcggttctt
    10441 attcaaagta ctcacatcgt accggatgaa ttttactctg acagagctgt agagcgaacg
    10501 tacagttcgt tgaattctct gagtgccatc aaatacatgg atatcagctt caaagaagta
    10561 aattccaatc agctggacgc taatatcatt cttactccca ataaggttca atcgctttcc
    10621 accgacttag aaggtaccta ttcagcaggc tactggggac tgggcggcaa catcaattac
    10681 ggacaccgga atatattcag aggctcagaa accttatcgc tccggggaag agccgcttac
    10741 gaatatcagg gcaaagatca gaatgcatac gaacttggtg gtgatatcgg tctgaaattt
    10801 cccacctttt tacttccgtt tgccacacgc gacataaaac gaaacgtaag agctaccacc
    10861 gaaataaacg gctcttacag ctacagaaac aggcccaaag aatatacggg aattgtgacc
    10921 ggaataggat tcaagtacaa ctggagcgaa cggtttgaaa ttaaacacaa tgttgacgta
    10981 ctgaacatca gttatgtgta ctacccgttt atttcacccg aataccgtgc ctacctgagt
    11041 acaagtccgt attttgtgta taattttcag aatcacctca tcatgcgaac cggttacagc
    11101 ggatcttaca gcagtttcag gcccttgcag ccacttcgga attacagcac gttaaactac
    11161 ggaatagaaa ctgccggtaa tttgctttat ggcatgaata atctgctgaa ctcaaaaaaa
    11221 gacacagcag gcactttcaa aattttcgga atcagatatg cgcagtttgt caaaaccgat
    11281 ttcaacgtga cccgccatca gatatttgat aaaaacaacc gtattgttta tcatgcagga
    11341 ataggagttg cactacccta tggcaataac aacctcattc ctttcgaaaa aagatacttt
    11401 tcgggcggag caaacagtgt acgcggatgg actgcttatc agctcggccc cggaacctac
    11461 aaaagcaaag gtgggtacat cgattacaac acgcaaatgg gcgacgtgag aatagatctg
    11521 aatatggaat accgaaccaa attattctgg aaactcgatg gggctttgtt tctggatgca
    11581 ggcaatgtgt ggaccatcag agagtacgaa agtcagccgg gaggagtttt tcgtttcagc
    11641 gatttcatcg gacaaatggg tgtggcttac ggaggtggac ttcgtgcgga tttttcattt
    11701 ttcgtgttga ggctggacct cgggttgaaa cttcacaatc cggcactga
//
LOCUS       DAORKP010000066        11704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_573_length_11704_cov_7.679543, whole genome shotgun sequence.
ACCESSION   DAORKP010000066 DAORKP010000000
VERSION     DAORKP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_11704_cov_7.679543"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..964)
                     /locus_tag="PLN75_05155"
     CDS             complement(110..964)
                     /locus_tag="PLN75_05155"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRG03539.1"
                     /translation="MKFNELTQEEKQVILYKATERPFTGELLDNKEKGTYVCKQCDAP
                     LYKSEAKFESHCGWPSFDDEIKGAVKRIPDADGRRTEIVCANCGGHLGHVFEGEGFTA
                     KNTRHCVNSISMKFVPEKTSTTETAYFASGCFWGTEYHFMKAPGVRATTVGYMGGRTQ
                     NPTYKEVCTGTTGHVETTEVLFDTSKTSYEDLIKLYFETHDFTQVGGQGPDIGDQYRS
                     VIFYVNEEQKKMAEKYIQILTDKGYKVATELKPAENNEFWDAEDYHQEYYDKKNGTPY
                     CHIYRKIF"
     gene            complement(1283..1753)
                     /locus_tag="PLN75_05160"
     CDS             complement(1283..1753)
                     /locus_tag="PLN75_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRG03540.1"
                     /translation="MWQRTQTIYFVLVILLMIAAVLIPNADFFNQANNITYRLDARGI
                     VELTQQGTTKGVVGTNPCTYLFGIILFLTTYCIASYKNRKRQLRMATINLFLIVIYIA
                     ILAVYIFFAKNKLEADITWKYPVIFPVIALIFNYLAMRGIAKDENLVKSLDRLR"
     gene            complement(1850..2839)
                     /locus_tag="PLN75_05165"
     CDS             complement(1850..2839)
                     /locus_tag="PLN75_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HRG03541.1"
                     /translation="MSEESEIKRKNDFFRKIKNLLISKDALVFLLFLALSGTFWFVQS
                     LDKQRETTLKIPVDYMGIPEDVELENKLPQRIEIKVRDEGLTLMKYNSRNTVPLALDL
                     ERVYFAKGKIVITADQLKNRISRYVLPTTAVLEIKPDSIVVLYHKLASVTLPVRLSGE
                     VKLSSQYVLSDSIQIEPSTVKIFGPKNIVDTMRAVYTEKLDTKSLTDTTFTKVRLEKP
                     KEGIRYAFSDVNVGIFVEMFTENKLEMPVTVINAPENINVRIFPPTVYVTYNVGLSNF
                     NKVRENDIKVIFDYKEAKDLDKRRYKLQIIQESHYISNLRVTPAQVEFLFEEK"
     gene            complement(2858..3199)
                     /gene="yajC"
                     /locus_tag="PLN75_05170"
     CDS             complement(2858..3199)
                     /gene="yajC"
                     /locus_tag="PLN75_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRG03542.1"
                     /translation="MNLLNMLLQTQAPAAPGQQGGMGAYSGILMMVLIFVVFYFFMIR
                     PQTKRQKEIKAQREAMKQGDRVVTSGGIYGKIRDIKETTVTVEIADNVRITVDKNSVF
                     ATAADIPAETK"
     gene            complement(3314..4249)
                     /gene="nusB"
                     /locus_tag="PLN75_05175"
     CDS             complement(3314..4249)
                     /gene="nusB"
                     /locus_tag="PLN75_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRG03543.1"
                     /translation="MINRVLLRIKILQIVYAYYKGHSASVALAEKELFYSVEKTYELY
                     FQLLQLAVEVTNYAALKIDARKNKLRPSAEDLNPNTRFVDNKFIHQLSRNVQLQSFID
                     EHKISWANNLDTVKMVYELTMESDMYAEYMSAETSDYAADKDIWRKIFKKLILQSEEF
                     ADSIEDQSIYWNDNLEIVISFIIKTIKRFDLASGVEQELLPMFKDDEDSEFAGKLLKN
                     TLEKEKEYREMIDIHTQNWELDRIAFMDIIIMQTALAEIMSFPTIPVNVTLNEYIEIS
                     KNYSTDKSAIFINGVLDNIVKELKAENKLIKVVKI"
     gene            4566..4997
                     /locus_tag="PLN75_05180"
     CDS             4566..4997
                     /locus_tag="PLN75_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRG03544.1"
                     /translation="MEVEKRKIELEKKDSEIIYSKAIKAGKRIYYLDVKKSRNDDLYL
                     AITESKKKVTGYDEDAQVTYEKHKIFLYKEDFEKFVEGLDDVIGFIKQEQGEPIPRPE
                     YNHSQNDSNVLAEEIAPEVKEITEPTTTGETKRSFFDKFKF"
     gene            5129..6154
                     /locus_tag="PLN75_05185"
     CDS             5129..6154
                     /locus_tag="PLN75_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRG03545.1"
                     /translation="MKTFASDNYSGVHPEIMQAIIQANSDHQISYGDDEITAEMNNIF
                     REQFGDVDVLYAFNGTGANIVSLKCAVLPFQSVICTESAHIQVDECGGPTQNIGSTLV
                     PLPAPDGKLTPAIIEHQLTFIGNVHHTQPRVISVSQSTELGTIYSRKELKALADFAHT
                     NDMYLHVDGARISNAVAALGCTLKEATADCGVDIMSFGGTKNGLMIGEAILIFNKELR
                     KHAPFYHKQTAQLFSKNRFIAAQFKALLSNDLWKRMAAHSNRMAQLLANETGNLEGVT
                     ITQKVQANAVFAIIPKHAVEPLRSAFRFYDWNAATGELRWMCSFDTTEEDVYEFVKKL
                     KELIAAT"
     gene            6277..7458
                     /gene="uxuA"
                     /locus_tag="PLN75_05190"
     CDS             6277..7458
                     /gene="uxuA"
                     /locus_tag="PLN75_05190"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRG03546.1"
                     /translation="MQKTWRWFGKNDPITLPMLRQIGVEGIVTSLYHIAPGKVWPEEE
                     IHNLNNFIKNQQLTWSVVESVPVAESIKYGGNDRDELIENFRQTLINLGKLGINTVCY
                     NFMPVIDWIRTDLNRTLPDGTSTLFFDKIRFGYFDLKILKRKNGEKDFTAEELQKIEE
                     LDRTITQEEKDQLIYTIIIKTQGFINRNIVGENQNPVDAFNSLLNLYQGIDKNQLREN
                     LLYFLKKVIPTAEEYGVKLCIHPDDPPFGAFGLPRIVSNEDDIEWILSAVNSPVNGLT
                     FCVGSLSAGIHNDVARLASRFVKRVHFAHLRSTEVLDNGDFLEASHLEGRGNLIEIIR
                     IFEKENPALPMRVDHGKLMLDDGDKNYNPGYSFHGRMFALAQVEGMMAVVRNELNAGI
                     Y"
     gene            7478..8290
                     /locus_tag="PLN75_05195"
     CDS             7478..8290
                     /locus_tag="PLN75_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRG03547.1"
                     /translation="MNKLFSIEEKVVVITGGTGILGGTIAAYLAEEGAHVVILGRRKE
                     AGDKLVSEIGQKGFEAIFLETDVMNKNALIDNRTEILKRYGRVDALLNAAGGNMPGAT
                     IPPTGTFFDLNIDDFEKVLRLNLTGTVLPTQVFLEPMVAQMKGSIVNFSSMAAFRPMT
                     RVAGYAAAKAGISNFTQYMAHEVALKFGEGIRVNAIAPGFFITEQNRNLLTNPDGTYT
                     ERGNDVIRQTPFRRFGKPEELCGTAQYLISDASSFVTGTVAVVDGGFNTFAM"
     gene            8317..9129
                     /locus_tag="PLN75_05200"
     CDS             8317..9129
                     /locus_tag="PLN75_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRG03548.1"
                     /translation="MTTGSTSIDLTNEQKELRLQYERLKIEFSELFALKNEMLSHEAP
                     LLTSLYLELIGRKLYEVYCLSVELSKLKQRMTLLQAYVNRNQIPEITVVDKKIETQFA
                     EYQKKIEDEAQRLAAAKEYLKGSFLSAEETKELKGIYYTIVKRLHPDINADLTEDQKE
                     LFVKAQAAYDLLDINTLRIILISLDIGKKEDSEEYNLAEKVKKLAENVSVLTNQIDAL
                     NREFPYVLREKLSDEAWIKAEQEDADKQLETYKTEIEKYKQYVILLEEWKPA"
     gene            9114..9503
                     /locus_tag="PLN75_05205"
     CDS             9114..9503
                     /locus_tag="PLN75_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HRG03549.1"
                     /translation="METGITKLSPELLALIGTGGVNALAPFTREIFLLDIVVAGTSYC
                     KKIDELKDELTPETVLKMIRDPENLHDELAIGIYFKDIRIGWVPRELNEVISRLMDAG
                     KAFFCRITKTRLINSWLKINAKIFMVE"
     gene            complement(9551..10546)
                     /locus_tag="PLN75_05210"
     CDS             complement(9551..10546)
                     /locus_tag="PLN75_05210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRG03550.1"
                     /translation="MNQSSISPANGGYGAFSTPVGIPLPAFEITYHDRIFTLGSCFAE
                     NIGKKLQDDYFLSFINPFGVLYNPFSVSNSIRYLVSDKLFGDDDLFHLGSLWNSFAHS
                     SIFSATSSEETLQKINSRLIAARDFLSQTKVMMITLGTAWIYEDSETGKVVANCHKLP
                     SARFNRRRLNVEEIVGELSEVMLQLREQIPDLQVIFTVSPVRHWKDGAHENTVSKSTL
                     HLAIDELVKKFSFTGYFPAFEIVMDELRDYRFYDTDMLHPSAQAVDYIWQRFSDLYFS
                     ENTRKLKKELEQLRADLNHRPLHADTIEYQQFILSVEKKKNGLIENYPFLKDRFE"
     gene            complement(10608..11522)
                     /gene="menA"
                     /locus_tag="PLN75_05215"
     CDS             complement(10608..11522)
                     /gene="menA"
                     /locus_tag="PLN75_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HRG03551.1"
                     /translation="MKKLQYYVQSARLRTLPLSVAGIIMGAMIAASDSHWSTATFVWG
                     LLTALSLQIISNVANELGDLQKGTDNENRLGPIRSVQSGKLTPQNLKGMLAVFVAIAM
                     FSGLMLVVVSFHSLTSFYSLLMLFTGALAIVAAVKYTFGVKAYGYVGLGDVFVFIFFG
                     LVSVLGMYFLLAGTFSPEVLLPASSIGFLSTAMLNQNNMRDIDNDKNFQKKTLAVRMG
                     LNAAKTYHLIIIVLAFILMTSYCFIEKKGIFGYLYLISLPVFVHHLNYVLKNDGKSLD
                     KHMKVIAAGTMLFAVSGGVGMILEQLMG"
BASE COUNT         3621 a         2404 c         2363 g         3316 t
ORIGIN      
        1 ggtctgacgg tctgtgaaaa gccaatattt acaattaata aaaatacaaa taacaggctg
       61 actttttttc tcataattat taaaatatcg tttttgaatt aagtttcttc taaaaaattt
      121 ttctgtaaat atgacaatag ggagtgccgt ttttcttatc gtaatactcc tgatgataat
      181 cctccgcatc ccagaattcg ttattttcag ccggtttcaa ttcggttgcc accttataac
      241 ctttgtccgt cagaatttgg atatactttt cggccatctt cttctgttct tcatttacgt
      301 aaaaaatcac cgagcggtac tggtcgccga tatcaggacc ctgaccgcct acctgtgtga
      361 aatcgtgcgt ttcgaaataa agctttatca ggtcttcgta gctggttttg gaggtatcaa
      421 agagcacttc ggttgtttcc acgtgcccgg tagtaccggt acaaacctcc ttgtacgtag
      481 gattctgcgt acgcccgccc atgtaaccga cagtagtggc acgtacaccc ggtgctttca
      541 taaagtgata ttctgttccc caaaaacacc ccgatgcaaa gtaggcagtt tcggtggtgg
      601 atgttttttc ggggacaaat ttcatggata tggaattaac gcaatgacgg gtattcttag
      661 ctgtaaatcc ctcaccttca aacacatgtc caaggtgtcc gccgcaattg gcgcaaacaa
      721 tttccgtacg tcggccatca gcatcgggaa ttcgtttcac agcacccttt atctcatcat
      781 cgaacgacgg ccatccgcag tgactttcga atttggcctc cgacttgtag agcggtgcat
      841 cgcactgttt gcatacatag gttccctttt ctttgttgtc cagaagttcg ccggtgaaag
      901 gtctttcagt ggccttgtaa agtattacct gtttttcttc ctgagttaat tcattgaatt
      961 tcattgcttt gttgttggtt ttattggttt gtccacaagc agtaaaaaca acgaggagca
     1021 tcaggattac cacctgccct ccccgtgtaa agtatgtttt cgttgctttc atatttctta
     1081 atttattagt tttaaaaaca aagaaagcgg cgaattgttt gattcaggaa attcgattat
     1141 tcgggtattc aattgttttg acgttccggg atattattta aattcaatct ctctaaaatt
     1201 caattctaca gactgcaaac agacatttaa acatccaaac tcccgatagc tatcgggacg
     1261 aacatcctaa ctttcgaaca ttttatctca acctgtctaa tgattttacc aggttttcgt
     1321 cttttgcaat tccacgcatt gccaggtagt tgaatatcag cgcaatgacc ggaaatatga
     1381 ccggatattt ccaggttata tcagcttcaa gtttgttttt ggcaaaaaag atatatactg
     1441 caagaatagc gatgtaaatt acaatcagaa acagattgat ggtagccatt ctgagctgac
     1501 gttttctgtt tttataagaa gcgatgcaat aagttgtcag aaaaagaata attccgaaaa
     1561 gataggtaca gggatttgta ccaactactc cttttgtagt tccctgttga gtcagttcta
     1621 ctattccgcg agcatccagc ctgtatgtaa tgttgttagc ctgattaaag aaatctgcat
     1681 tgggtataag cacagcagca atcatcagaa ggattaccag tacaaaataa atcgtttgag
     1741 ttctctgcca cataaatttt aaaagttgat agttgaaatt actttttttc cggaaagtgg
     1801 tatagtatga aatggtttta tttgatttgg aaatctgatt tggagtatgt tatttttctt
     1861 cgaacaaaaa ctctacctgc gcgggtgtaa ctcgaagatt cgagatataa tgtgattcct
     1921 gaataatctg cagtttatac cttcgtttat cgagatcttt ggcttcttta taatcaaaaa
     1981 tcactttaat gtcattttct ctaactttgt taaaattgct cagaccgacg ttgtaggtaa
     2041 catacacagt gggtggaaaa atccgcacat taatattttc gggagcattg ataaccgtca
     2101 ccggcatttc cagtttattc tcggtaaaca tttcgacaaa gattccgacg tttacatcac
     2161 tgaaagcata cctgatgcct tcttttggtt tttcaagtct cactttggtg aaagtagtgt
     2221 cagtcagcga tttagtgtcc agtttttcgg tatatacagc tctcattgta tctacaatat
     2281 ttttcggacc gaagattttg actgttgagg gttctatctg tatgctgtca ctcagtacgt
     2341 attgcgacga aagttttact tcgccactca gtctgacggg caaagttaca gacgcaagtt
     2401 tgtgatacaa taccacaatt gaatcgggtt taatttccag aacggcagtt gtagggagta
     2461 cgtatcgcga tattctgttt ttcagctggt cggcagtaat aacgattttt ccttttgcaa
     2521 aataaaccct ttcgaggtcg agtgcaagcg gcaccgtgtt tctgctgttg tacttcatca
     2581 acgtgagccc ttcgtcacgc actttaatct ctattcgttg cgggagtttg ttttccagtt
     2641 ccacgtcttc aggtattccc atgtagtcca caggaatttt cagcgtggtt tcacgctgtt
     2701 tgtcaagcga ttgtacgaac cagaatgttc cggagagggc aagaaacaac aggaaaacca
     2761 atgcatcttt tgaaattaaa agatttttga ttttcctgaa aaagtcattt ttgcgtttta
     2821 tttctgactc ttcggacata attcatcaga aggtaggtta tttcgtttct gcaggtatat
     2881 cggcagcagt agcaaaaaca gaatttttat ctacagtaat gcgcacatta tcagctattt
     2941 ctacagttac agtagtttct ttgatatcgc ggattttgcc gtaaatacca cctgaagtca
     3001 ctacgcggtc gccttgtttc atagcttcgc gttgggcttt tatttctttc tgacgtttag
     3061 tctgcggacg tatcataaag aaataaaaca caacgaaaat aagcaccatc atcaggatac
     3121 ctgaataagc tcccattccg ccttgttgtc ccggggcagc aggggcttgt gtctgtaata
     3181 gcatgtttaa taagttcatt tttataaagt tagaaattag aagttagaaa ttagaaactt
     3241 ttgtcagatt agaaatcaga agaaaagaag aattttgaag caatcattct gttaaacatt
     3301 ctgcttactg ctttcaaatc ttcactactt tgattagttt attttcggct ttcagttctt
     3361 tcactatgtt atcgagcact ccgttgatga aaatggcact tttatcggta ctgtaatttt
     3421 ttgaaatctc aatgtattca tttaacgtta cattcaccgg aattgttggg aatgacatga
     3481 tttcggccag tgcagtttgc ataatgatga tatccatgaa agcaatccgg tccagttccc
     3541 agttttgggt atgaatatca atcatttcac ggtattcttt ctctttttcg agtgtatttt
     3601 tcaaaagttt gccggcgaat tcagaatctt cgtcgtcttt gaacatcggc aacaactcct
     3661 gctccactcc acttgccagg tcaaatcttt tgatggtttt tataatgaaa ctgataacta
     3721 tttcgagatt atcgttccag taaatgctct gatcttcaat ggaatctgca aattcttcgc
     3781 tttgaagaat taatttttta aatatttttc tccagatatc cttatcggcg gcataatctg
     3841 atgtttcggc cgacatatat tcagcataca tgtcagattc catggttagt tcatatacca
     3901 ttttcacggt atccagatta tttgcccagg atattttatg ttcatcaatg aaactttgca
     3961 gctgcacatt ccgggacaac tgatggataa atttattatc gacaaaacgg gtgttgggat
     4021 tcaaatcttc cgcactcgga cgaagtttgt ttttacgtgc atcaattttg agggcagcgt
     4081 aattggtaac ttccacagcc agctgcaata attgaaaata aagctcgtaa gtcttttcaa
     4141 cactataaaa caattccttt tctgctaaag ccacagaagc gctgtgcccc ttgtaataag
     4201 cataaacaat ttgaaggatc ttaatacgaa gtaaaactcg gttaatcata aattctctga
     4261 ttcgtcaatc ttaaatatga tgcaaaggta caaaactttt ctgttcattc tgtcatttca
     4321 cttctaaata tctgaaatat gatgcatttt cggtactttc aaaatagttt aatttcaaaa
     4381 aaaacacaat taatcttctc tttgtttgaa taatcgaaaa aatattctga aatttggcaa
     4441 tcgaaagaac aaacaataat tctatcggtt tttttgaata aaaattaata ctaacgcagc
     4501 taagccagga tattttacgg atgtgaaaat tacattgaca gctgttaaaa aaaattatct
     4561 gatttatgga agttgaaaaa agaaaaattg agttggaaaa aaaggatagt gaaattattt
     4621 actccaaagc catcaaagca ggaaaaagaa tttattatct cgatgtaaag aaaagcagaa
     4681 atgatgattt gtatcttgca atcaccgaaa gtaagaaaaa agtaacagga tacgatgaag
     4741 atgcacaggt aacgtatgag aaacacaaaa tattcttgta caaagaagat ttcgaaaaat
     4801 ttgtagaagg actcgatgat gttatcggat ttatcaaaca agaacaggga gaaccgattc
     4861 ccagacccga atataatcat tcgcagaatg actccaacgt attggctgaa gaaattgcac
     4921 cggaagtaaa ggaaataact gaaccaacaa ctacaggaga aacaaaacgc agtttttttg
     4981 acaaattcaa attctaaaca atttttttaa acaatagaat aacgtccgtg gaaatctatg
     5041 gacgtttttt cattcagatg aatctatttg tatttatatc aacaaaatca aactaatcaa
     5101 actaatcaaa ttaatcataa ttctgacatt gaaaacattt gccagtgaca attattcggg
     5161 tgttcatccc gaaatcatgc aagccatcat ccaagccaac tctgaccacc agatatcata
     5221 cggcgacgat gaaataacgg ctgaaatgaa taatattttc agagaacaat tcggtgatgt
     5281 ggatgtgctt tatgcattca acggtaccgg tgccaacatt gtaagtttga aatgcgctgt
     5341 attgcctttt caatcagtga tttgtactga atcggctcac attcaggtag atgaatgcgg
     5401 aggacctacc caaaacatcg gtagtacact ggtgccgctg cctgcacccg atggaaaact
     5461 tactcccgca atcatagagc atcagcttac tttcatcggc aatgtacacc acacgcagcc
     5521 cagggtgatt tctgtaagtc aaagcacaga actcggcacc atatacagtc ggaaagagct
     5581 caaagccctg gccgattttg cacatacaaa cgacatgtat ctccatgtgg acggagcacg
     5641 catctccaat gccgtagcag ctctgggatg tacgctgaaa gaagctacag cagactgcgg
     5701 agtggatatc atgagtttcg gcggtaccaa aaacggactg atgattggcg aagcaatact
     5761 aattttcaat aaagaactcc ggaaacatgc acctttttat cacaaacaaa cggcacaact
     5821 attctccaaa aaccggttta tagccgcaca gttcaaagca ctgctatcca acgatttatg
     5881 gaaaaggatg gctgcacatt ccaaccggat ggctcaactc ctcgccaatg aaacaggcaa
     5941 cctggaaggt gtaaccatta cccaaaaagt acaggcaaat gccgtttttg cgattattcc
     6001 gaaacatgcg gtagaacctc tccgaagtgc tttccgcttt tacgactgga acgctgcaac
     6061 cggtgaactg cgctggatgt gctcatttga cactaccgaa gaagatgtgt atgaatttgt
     6121 taagaaactg aaggagttga tagctgccac atgactacga tgagataaaa aaaatgtcaa
     6181 tcacctgaca tacaagatat taattgttat tttaaaaata gtttaagact taaaacaaac
     6241 tctctgataa ttccccgttc agggacaaat cttattatgc aaaaaacctg gagatggttc
     6301 ggtaaaaacg acccgataac gcttccgatg ctcagacaaa tcggtgtgga aggaattgta
     6361 acttcactgt atcatattgc acccggaaaa gtctggccgg aagaagaaat tcacaatctg
     6421 aataatttta ttaaaaatca acaacttacg tggagcgtgg tcgaaagtgt tcctgtagcc
     6481 gaatccatta aatacggagg aaatgacaga gacgaactga ttgaaaattt ccggcaaacg
     6541 ctgattaatc tgggcaaact cggcatcaat actgtatgct acaacttcat gccggtaata
     6601 gactggatac gtaccgattt aaaccgaacg cttcccgacg gcacatcaac actgtttttt
     6661 gataaaatcc ggttcggcta tttcgatttg aaaatcctga aacgaaaaaa tggcgaaaag
     6721 gattttacag ccgaagaact tcaaaaaatt gaagaactgg acagaaccat cacgcaagaa
     6781 gaaaaagacc agctgattta caccattata attaagactc agggatttat caaccggaat
     6841 atagtcggtg aaaatcaaaa tccggtagat gcctttaatt cccttctgaa tttatatcag
     6901 ggaatcgaca aaaaccagct tcgcgaaaat ctgctctatt ttctgaaaaa agtgattcct
     6961 acagcagagg agtatggcgt aaagctgtgt attcatcctg acgatccacc tttcggagca
     7021 ttcggtctgc cccgcatagt gagcaatgaa gatgacattg agtggattct ttcggcagtg
     7081 aacagccctg tcaatggtct gactttttgt gtcggatcgc tgagtgctgg catccacaac
     7141 gatgttgcgc ggctggcttc cagattcgtg aaacgagttc actttgccca tttgcgaagc
     7201 accgaagtgc tcgataacgg cgattttctg gaagcatcgc acctggaagg acggggaaat
     7261 ctaatcgaaa taattcgtat ctttgaaaaa gaaaatccgg cattgcccat gcgggtggac
     7321 catggaaaac tgatgctgga cgacggagat aaaaattaca atcccggcta ttcatttcat
     7381 ggaagaatgt ttgccctggc acaggttgaa ggcatgatgg ccgtagtaag aaacgaatta
     7441 aacgcaggaa tttattaaac aaaaaacgca tttgataatg aataaattat tcagcattga
     7501 agaaaaagta gtggttatta ccggaggaac aggcattctg ggcggaacca tagctgccta
     7561 tctggccgaa gaaggcgctc acgtagtgat tctgggcagg cggaaagaag ccggcgacaa
     7621 actggtgagt gaaatcgggc aaaagggatt cgaagccatt ttccttgaaa cagatgtgat
     7681 gaacaaaaat gcactgatag ataaccgaac cgaaattctg aaacgctacg ggcgcgtgga
     7741 tgcattgctc aacgctgcag gaggcaatat gccgggtgca accatacctc cgacgggaac
     7801 ttttttcgat ttgaacatcg acgattttga aaaagtgctt cgtctgaatc tgaccggaac
     7861 ggtattgcca actcaggtat ttctggagcc gatggtagca cagatgaaag gaagcattgt
     7921 gaatttctca tccatggctg ctttcagacc catgacccgc gtagccggat atgctgcagc
     7981 caaagccgga atctcaaact tcacccaata catggctcac gaagtggcac tgaagtttgg
     8041 tgaaggaatt cgggtaaatg ccattgctcc cggatttttc attaccgagc aaaaccgcaa
     8101 tctcctcaca aatcccgatg gcacctacac cgaaagaggc aacgacgtaa tcagacaaac
     8161 tcccttcaga cgattcggta aacctgaaga actctgcgga actgcacagt atttaatcag
     8221 cgacgcttca tcgttcgtta ccggaacggt ggcagtggtc gatggcggat tcaatacgtt
     8281 tgccatgtaa tttttttcaa aaaaaaactc caaataatga caaccggctc cacttcaatt
     8341 gaccttacaa acgaacaaaa agaactccgg ctgcaatacg aaagactgaa aattgagttt
     8401 tcagaactat ttgcgttgaa gaacgaaatg ctgtcacacg aagcaccgtt actcacatcg
     8461 ttgtaccttg aactgattgg aagaaaactg tacgaggtgt attgcctgag cgtggaacta
     8521 tcgaaactca agcagcgcat gactctgctg caggcttatg tcaacaggaa ccaaataccc
     8581 gaaattactg tagttgacaa aaaaatcgaa actcagtttg ccgaatatca gaaaaaaatt
     8641 gaggatgagg ctcaaagact ggcagcagct aaggaatatc tcaaaggctc attcctgagt
     8701 gctgaagaaa cgaaggaact gaaaggaatt tattacacca tcgtaaaaag actgcatccc
     8761 gacatcaatg ccgacctgac agaagatcag aaagaactgt tcgtaaaagc tcaggcagca
     8821 tacgacttgc tcgacatcaa tacgttacgg atcatattga tatcactcga catcgggaaa
     8881 aaggaagatt ccgaagaata caatctggcc gaaaaagtga aaaaactggc tgaaaatgta
     8941 tctgttctga cgaatcaaat cgatgcgcta aaccgcgaat tcccgtacgt gctgcgcgaa
     9001 aaactaagtg acgaggcatg gataaaagca gaacaggaag atgccgacaa acaactggaa
     9061 acatataaaa ctgaaataga aaagtacaaa caatacgtaa tcctgctcga agaatggaaa
     9121 ccggcataac caaactttca cccgaactac tggctttgat tggcaccggc ggagtaaatg
     9181 cgctggcacc tttcacgcgc gaaatcttcc tgctggatat agtggtggca ggaacttcgt
     9241 attgtaaaaa aatagatgaa cttaaagatg aactgactcc tgaaacagtg cttaaaatga
     9301 ttcgcgatcc cgaaaacctg cacgacgaac tggctatcgg tatttatttc aaagatatcc
     9361 gaattggatg ggtgccaagg gaactgaacg aagtgatttc gcgattgatg gatgccggaa
     9421 aagctttttt ctgccgcatt accaaaacca gattgataaa cagctggctg aaaataaacg
     9481 ccaaaatatt tatggttgaa tagctttttt taaaattcaa gaattttcag ttggattatt
     9541 ttggtattaa ctactcaaat ctatctttca gaaacggata attttcaatt agtccgtttt
     9601 ttttcttttc cactgataaa ataaactgct gatattcaat tgtatccgca tgaagggggc
     9661 gatgattgag gtctgccctg agctgttcca gttctttctt cagtttccgg gtgttctccg
     9721 aaaaataaag atccgaaaac cgctgccaga tataatccac cgcctgagcc gatggatgaa
     9781 gcatatcggt gtcataaaac cggtaatcgc gcagctcgtc catcacaatt tcgaaagcag
     9841 gaaaataacc ggtaaacgaa aactttttca ccagttcatc tatagccaga tgaagcgtac
     9901 ttttgcttac ggtattctca tgggcgccgt ctttccagtg acgaacgggg cttaccgtga
     9961 aaattacctg caaatcaggg atctgctcac gcagctgcag cataacttcc gatagttcac
    10021 ctacaatctc ttccacgttc agcctgcggc ggttgaaccg ggcagatggt agtttatgac
    10081 agtttgcaac cacctttccg gtttccgaat cctcgtaaat ccaggctgtt ccgagtgtta
    10141 tcatcattac ctttgtttga ctcaggaaat cgcgggcggc aatcaggcgg ctgttgattt
    10201 tctgcaaagt ttcttcagac gaagtggccg agaaaatact gctgtgagca aaactgttcc
    10261 acaaagagcc caggtgaaat aaatcatcgt caccaaacag cttatcgctc accaaataac
    10321 gtattgagtt gctcaccgaa aatggattgt acaacacacc aaacggattg ataaacgaca
    10381 aaaaataatc atcctgcagt tttttgccaa tattttcggc aaaacacgat ccaagcgtaa
    10441 aaatccggtc gtgatacgtg atttcaaaag caggaagcgg aataccgacg ggagtggaaa
    10501 acgcgccgta tccgccatta gcgggtgata tggatgattg attcatattt tttcgtatcg
    10561 acaaattact atccgttttt tataagtccg cgtttaaaaa taccggttca tcccatgagt
    10621 tgttctaaaa tcatacccac tccacctgat acggcaaaca acatggtacc tgctgcaatc
    10681 actttcatgt gtttgtccag gcttttgcca tcgtttttca gcacataatt taaatggtgg
    10741 acaaataccg gtaatgaaat cagatacaaa taaccaaata ttcccttttt ttcaataaag
    10801 caataagatg tcatcagaat gaaagccagt acgatgatta taagatgata agttttcgca
    10861 gcattcaaac ccatgcgtac agcgagggtt tttttctgaa aatttttatc gttatcaata
    10921 tcgcgcatgt tgttttggtt gagcatggcg gtggaaagaa atccgatgga tgaagccggc
    10981 agtaatactt cgggcgaaaa agttccggcc agcagaaaat acattcccaa aacactcacc
    11041 aatccgaaga aaataaaaac gaatacatcg cccaacccaa catagccgta ggctttgaca
    11101 ccgaacgtgt atttgacggc tgcaacgatg gccaaagcac cggtgaatag catcaaaaga
    11161 ctgtaaaatg aagtaagtga atggaatgaa actactacga gcatcaagcc ggaaaacatg
    11221 gctatggcta caaacactgc aagcatccct tttaggtttt gcggagtcag tttgccgctt
    11281 tgtacactcc ggatgggtcc cagccggttt tcgttatcgg ttcctttctg caaatcgccg
    11341 agttcgttgg caacatttga tataatctgg agcgaaagtg cggtaagcaa accccaaaca
    11401 aatgttgcgg tactccagtg gctatctgag gcagctatca ttgctcccat gatgataccg
    11461 gcaacagaaa gtggcaacgt tcgcaacctg gcactttgga cataatattg taattttttc
    11521 acctgtaaaa atactgtttt ttcgaattgc aaagttaaga aaaacgggga acataaccac
    11581 taaaaaaaaa cagagacgtc atctgaaaaa tgacatctcc gcctgtaaat ccggacttac
    11641 ttaatccgta tttcttattg agccttaact acaacttttt tttgctttcg cagcaagcag
    11701 attt
//
LOCUS       DAORKP010000067        11652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_579_length_11652_cov_8.047857, whole genome shotgun sequence.
ACCESSION   DAORKP010000067 DAORKP010000000
VERSION     DAORKP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_11652_cov_8.047857"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLN75_05220"
     CDS             complement(<1..398)
                     /locus_tag="PLN75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRG03552.1"
                     /translation="MKYALLLTQACNLACRYCYIIKHKHSISTENARKAVDFIFSNSQ
                     EGEPIDIGYFGGEPLLEFNRLKEITEMIEKHPLFPLREVVLQVITNGTIFSDEMAGYL
                     LQHNISLGVSCDGIPAVQDQARIYPDGRPSS"
     gene            complement(500..880)
                     /locus_tag="PLN75_05225"
     CDS             complement(500..880)
                     /locus_tag="PLN75_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03553.1"
                     /translation="MEYIRYSPVFDRHLINGPHNPMFWRYKNIVEKFVKEMKLEPIHE
                     EMIQKRSIELNPLHLDLEWWWKYGGMKMAHVHLNGQIYLLKDEQWREFSKRILTDIQN
                     KLKDAKGVSFENTLDIVNSMESIF"
     gene            complement(1629..3338)
                     /locus_tag="PLN75_05230"
     CDS             complement(1629..3338)
                     /locus_tag="PLN75_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03554.1"
                     /translation="MKCFLFGILLVLTANSAACYTVFHGNSALQNGNDSSVTCRQQLP
                     GLQEAIAGGLLYLRERQISSRRVKLQPDDFRLTRTRKTYGINSIRSFLGTWLFTEKHQ
                     QGAWPGFVTFLPTRGPAAWKPITTATDYNLFTTASTLYCLYRFDESLLPSGSRFVANM
                     RNNALVTFGHFRHNDTYRFWKIRRTELVSYPYTAPENIPLWMLSVRQWINKKTGWWGL
                     NGFHESDYISEWIHRIYNPVYNKTGARAMFNIPADTDDTSLAVTMLLLDCNDNPQREA
                     DSVALAQLNRYRDQNRLRRDRWNSHTGENTGAFLTWHKDENTPVFDQPDMGIIPLENN
                     NVDLVVNCNALLALGAAGMTHTPRYAEVLKLLSRAVKYNLWQRGNIYYPEPLWFPYAL
                     SRAISEADGGIPEADTLFIQLTEQLLRMEQKQSRSHPESAGSFAPFDSVSTVTATALG
                     LNALLNMGRKYAVTTGMTAAYDSIVNASARYLLESRKSYKTKTYPNEICYWQSGPLFS
                     SSVHELAYWYSNSLNTALVIDALSKYVAYTDYSPETRSKKKLPIYIINGKYSVDFSAN
                     SYQ"
     gene            3654..5567
                     /gene="dxs"
                     /locus_tag="PLN75_05235"
     CDS             3654..5567
                     /gene="dxs"
                     /locus_tag="PLN75_05235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRG03555.1"
                     /translation="MAKQNYKYLNSINSPDDLKRIDKNELHVVCDELRDFIIDEVSKN
                     PGHLGSSLGVVELTVALHYVFNTPYDRIVWDVGHQAYGHKVLTGRRDVFHTNRQLGGI
                     SGFPKIEESEYDTFGVGHASTSISAALGMAVAASMRGEDRKVVAVIGDGSMTGGLAFE
                     GLNNTSMDKNDMLIILNDNQMAIDPINGGFTQYLVDITTSKTYNKIRHKVYNLFRKSR
                     LVDDEKKNKIIRFNNSLKTAFTQKDHNIFEGLNIRYFGPVDGHDVSNLIRILAEIKEF
                     KGPKVLHVITQKGKGFEPAEKSATVWHAPGKFDPETGERLVCNHNNAEPPLFQDVFGK
                     TLLELAKQNDKIIGITPAMPSGCSMTIMQRRFPERVFDVGIAEAHAVTFSAGLAKEGM
                     VPFCNIYSSFMQRAYDSVIHDVAIQKLPVIMCLDRAGIVGSDGATHQGQFDLAYMRCI
                     PNLTIAAPRNEMELRNLMYTAQLPGKGPFVIRYPRGKGFNVNWHNEWRELTTGRGECL
                     KTGTDLAVITIGTMAAVAEKAINSVENLTGKSIAHYDARFLKPLDEEMLREIGEKFTK
                     VITIEDGTIEGGLGSAVLEFMSDINYHPEVLRIGIPDVFVEHGTPEELHAMLGMNAEG
                     VEKKIKDMIDAKV"
     gene            5646..6881
                     /locus_tag="PLN75_05240"
     CDS             5646..6881
                     /locus_tag="PLN75_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HRG03556.1"
                     /translation="MKLKTTLLIFSFVLTHTLLSQNQSQLAPTPPMGFMTWNYFGLDI
                     HENDIKTLADAMVETGLRDLGYNYIFIDDGWQGGRDNKNNIIADPAKFPSGMKSLVDY
                     VHTKGMKIGIYSDAAPLTCGGYTASLNFEEQDAKTFAAWGFDYLKYDYCGAPADWQTA
                     ISRYERMAKALQNSGRDIAFGICEWGDRSPWLWARKAGGHLWRTTADVRDKWKSHAPA
                     GSAPHELHGYGAGILDILEINAGLDKYAGPNGWNDPDMLVVGLYGKKGAPSTGLGGTG
                     CTDTEYQSQMSLWCLMAAPLMITCDVRNMNEATKRILTNKDIIAIDQDPLGIQAERKL
                     KTDLLQIFVKPLSNGDVALGILNTSDQEQTIEVNPETLGIFNKRKAKDLWSGQTMKTG
                     KRIKVKMAAHETKVFRLSE"
     gene            6984..8318
                     /gene="trkA"
                     /locus_tag="PLN75_05245"
     CDS             6984..8318
                     /gene="trkA"
                     /locus_tag="PLN75_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRG03557.1"
                     /translation="MKIIIAGAGDVGTHLAKLLAKENFDTTLLDEDPNRLTDLEMNYD
                     LLTRTGSPTSIESLKECGVEKADLFIAVTPYESINMTSCMLATNLGAKRTLARIENYE
                     YLIPENKEFFAKLGVDYLILPEKLAAEEIVESIKYTWLRQNLSFHNEALFLLAVKLRE
                     NALILNKKFNTGFFDHGKFRVVAINRNGHTIIPNGGDELMANDVVYFVTMKEHIQFVK
                     EQAGKKDYQIRNVMIMGGSRIAQKTIQYFPGNINVKVMERDKEKCYLLAGCLHNALII
                     NADGRNVEALKSEGIEEIDAFIATTSNSEANILACLEAKRLGVKKTVAEVENIDYIQL
                     AEELDIGSVINKKMIAASYIYQLTLDADVLDVKSLTSADAEVVEFVAKAGSKITKSQI
                     KNIRLPENVNIGGIVRDGVGSIAYGNTQILPGDHVMVFCVSSAIRKVESMFN"
     gene            8360..9832
                     /locus_tag="PLN75_05250"
     CDS             8360..9832
                     /locus_tag="PLN75_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRG03558.1"
                     /translation="MNRDLNIKLISRVLGGLLLIEAAFLLTPIAVALMYGETEMIRYY
                     LFTMLIALVLGLSGIAAGWKAESTVGKREGAVIVTSTWLLYTLIGSLPFWMSGFIPDF
                     TDAFFETLSGFTTTGTSILNNIESLPYNLLFWRSMTHWIGGLGIVVIFLAILPMIGGA
                     GYQLFTSESTTPTREKILPKINQTAKILLYVYVVLTLLCALALLLAGMNGFDALNHAL
                     STISTGGFSTKQLSIAYFQSPAIEYVIIFFMFFSGVNFTLYYFLVKFNFDKIIKNEEL
                     KYYIIVLVVAAVIIFLSHIDYNNSSGFRDFEKSFRSSLFIVTSCITSTGLCTEDYTLW
                     NSFTWVVMLVLMISGASTYSTSGGIKISRVVLIVKFCYYEFKKLVHPNAIIPVKFNGQ
                     IVKDEMMMRILAFTMIYTLIIVIGTFVLSLSGLGFIESMSGMITCMSDVGLGLGQLGP
                     THSFSEIPVFSKWFLAFVMIVGRLEVYSVLLLFTPEFWKK"
     gene            9835..11313
                     /locus_tag="PLN75_05255"
     CDS             9835..11313
                     /locus_tag="PLN75_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRG03559.1"
                     /translation="MPFKSEINFKLVSRLIGNLLLVESATMLFVLVITLLFNEDDGRY
                     FIYSSLIALALALLMILFGRNAPARAGKREGSMIVTFAWVFFSAIGALPFWMSGAIPS
                     YTNAFFETMSGFTTTGASILDNIEELSHGILFWRAMTHWLGGLGIVVISMAILPMLGI
                     NGVHLFAAETTGPTKDKFSPKVNDTARILFIVYIFLTIAETVFLYFGGMSLFDSVTHS
                     FSTVATGGFSTKQTSIAYWENSPYIQYVLAFFMLLSGVNFAMYFFGYKRKFFKIKENE
                     ELRYYLYIMIGASLMVMISLIDFSDTINLSVIEKAWRDSFFTISSLMTTTGFVTVDYM
                     TWKPVTWVVIVIIMLIGASAGSTAGGIKVVRIVIAAKACYYEFKRIIHPSAVIPVRYN
                     KIVVHDNTMIRVLTFIILYLSVTLIGILVLMISGMGISESIGGMITCISCVGPGFETV
                     GPSGNFNNIPEFSKWFLSFVMLMGRLELYTVLMILTPAFWRH"
BASE COUNT         3211 a         2507 c         2665 g         3269 t
ORIGIN      
        1 gaactgggcc tgccatcggg gtatatccgg gcctgatcct gaacagccgg tattccatca
       61 caacttacac ccagtgaaat attatgttga agtaaatatc cggccatttc gtcggaaaat
      121 attgtcccgt ttgtgataac ctgcagtacc acttctctga gtggaaaaag cggatgcttt
      181 tctatcattt ccgttatttc tttcagccgg ttgaattcca gcagcggttc gccgccgaaa
      241 taaccaatat caatcggttc accttcctga gagttggaaa agataaaatc aacggcttta
      301 cgtgcatttt cagtacttat cgagtgtttg tgcttgataa tataacagta ccggcaagcc
      361 agattacaag cctgagtaag tagtagtgcg tatttcatgg gtaaaaaagt ttggataaaa
      421 cccggtttgg aaatgaataa ttcgtgcaga ggtaatacaa acccgcctta ccgtttcggg
      481 caggcgggtt tacagggggt caaaagattg attccattga attaacaatg tccagggtat
      541 tttcgaaact tacacctttt gcgtctttta gtttattctg aatatctgta agaattcttt
      601 ttgaaaattc tctccattgt tcgtctttca gtaaatagat ttgaccattc agatgcacat
      661 gagccatctt cattcctcca tatttccacc accattccag atctaaatgt agcggattta
      721 attcgatgga tcttttttga atcatttctt catgaatcgg ctccagtttc atttctttca
      781 caaatttctc aacgatgttt ttataacgcc aaaacatcgg attgtgagga ccgttgataa
      841 gatgacggtc gaaaacaggt gaatatctga tatattccat tttatttgaa aaattaattt
      901 tagtttgata ataatcagtt tgtgctaact gatatagaaa aagttacggt ggattatccg
      961 gatgcttttt caggtatcaa tacaggggga gagagtaatc aatgcgtttt aaggcaaccg
     1021 gtattcacag tagtttatgc tgtgcgtcaa attgaaattg tattaaatac tgtatttatt
     1081 gccttatgct gattttcgat gcacggatac aggtaaatta atatacctgt tgcttttgaa
     1141 atatattgta taagaatgtt ttttaaaacc gaaaatgtat tttgagttaa taaacactcg
     1201 gcttcttgtc gtttgcagtt gtgggtaaaa ctatacccga atgtaccgaa gaatagatta
     1261 cggtagctga ataaagttgt tttttttctt tctgccgttt acctcctttc tttctgaaca
     1321 agttcattaa caacaagata attataaaaa ttaaacaatt tgagaatctg attagttcac
     1381 tgacacattc atttctttgt ccactgatta accggactaa tttgaatgct cttttcagga
     1441 cattttcaat ctgtatcatc cggaattgcg tggaaacagt ccaaccgctt caggcggttt
     1501 gaccgatgag taaaaaatgt ccccgggacg gaaaaagatc tccctttggg ggagtttgag
     1561 gggtgctttc ttcccaaaaa gctcctcctt cgggcttgcc agcctcagaa gggggaatct
     1621 gaacgggatt attgatagga atttgcggag aaatccaccg agtattttcc attgatgatg
     1681 tatatgggga gtttcttttt gctccgggtt tccggactgt aatcggtgta agccacatat
     1741 ttagagagag catctatgac cagcgctgta ttgagggaat tagagtacca gtaagccagt
     1801 tcgtgtaccg atgaggaaaa cagcggtccc gattgccagt agcagatttc gttgggataa
     1861 gtttttgttt tgtaactctt ccggctttcg agcaggtatc gggccgaagc attcacgatg
     1921 ctgtcgtaag cagctgtcat tccggttgtt acggcatact ttctgcccat attgagcagg
     1981 gcgttgaggc ccagagcagt ggcagtgacg gtgctcaccg aatcgaaagg ggcaaacgag
     2041 cctgctgatt cagggtggct gcgactttgc ttttgctcca tgcgcaggag ttgttccgtc
     2101 agttgtatga aaagagtgtc tgcttcgggt atgcctccat ctgcttcact gattgctctg
     2161 ctcagagcat aaggaaacca aagcggttca ggatagtaga tattgccccg ctgccagagg
     2221 ttatatttta cagcacggct caacagcttc aacacttcag cgtagcgggg agtgtgagtc
     2281 attcctgctg cgccgagtgc caaaagggcg ttgcagttca ccaccagatc cacattgtta
     2341 ttttcgagcg gaataatgcc catatcgggc tgatcaaaaa cgggagtgtt ttcgtccttg
     2401 tgccaggtga gaaatgcgcc ggtattttcg cccgtatggg agttccatcg gtcgcgacgt
     2461 aacctgttct ggtcgcggta acggtttaat tgagcaagtg ccacgctgtc cgcctcgcgt
     2521 tgaggattat cgttgcagtc gagcagcagc atggtcacag ccagcgatgt atcgtcggtg
     2581 tcggcgggga tgttgaacat ggctctggcg ccggttttat tgtaaacagg attgtaaatg
     2641 cggtgaatcc attccgaaat ataatccgat tcatgaaatc cgttcagtcc ccaccagccg
     2701 gtttttttgt ttatccattg ccttacgctg agcatccaga gtggaatatt ttcgggtgcg
     2761 gtgtatggat acgataccag ctctgtccgg cgaattttcc agaaacgata tgtgtcgttg
     2821 tgcctgaaat ggccgaaggt aaccagggcg ttgtttctca tgttggccac gaaccggctt
     2881 ccggatggga gcaggctttc gtcgaaacga tagaggcagt acagtgtgga ggcggtggta
     2941 aagaggttgt aatcggtggc agtggtaatg ggtttccagg ctgccggccc cctggttgga
     3001 agaaaagtta cgaagccggg ccatgcaccc tgttgatgtt tttcggtgaa aagccacgtt
     3061 cccagaaaac ttcgtatgct gtttattccg tatgtttttc gggtgcgggt aagtctgaaa
     3121 tcatcgggtt gcagttttac cctgcgactg cttatctgcc tctctctgag gtaaagcagc
     3181 ccgcctgcta tggcttcttg cagtccgggc aactgctggc ggcaggtgac ggaggagtcg
     3241 ttgccgtttt gcaatgcgga gttaccgtga aaaacagtgt agcaggccgc tgaattagct
     3301 gtaagcacca gtaaaattcc aaaaaggaag catttcatgg tcatgttttg agtgtgatac
     3361 ccgcttgtgt tttcaagagt taaataattc cggttctcac gggagaatcc tgtttttcta
     3421 atcctgattc tgcaggatga accgttccaa agataattaa tttttgctga aaatatccag
     3481 aattcacaac taaatgaaaa taagaagtat taactgtaag aaaaagcaac acatctgcgc
     3541 aatccatctc ttttcgccgc tctttttacc agtttccaaa taaactttga ctaaagaatg
     3601 tcaaagcaat aaaaatgtat tatttttgca tgacagatac tttaaaaatt ggaatggcaa
     3661 aacaaaacta taaatacctg aacagcatca actcacccga tgacctgaaa agaattgaca
     3721 aaaacgaatt gcatgttgtg tgtgacgaac tgcgtgattt tattatcgac gaagtttcaa
     3781 aaaaccccgg acacctgggc tcaagccttg gcgtggtgga acttacggta gcactgcact
     3841 acgttttcaa tacgccttac gaccgcatcg tgtgggatgt gggacaccag gcttacggtc
     3901 ataaagtgct taccgggcgg cgcgacgtat tccataccaa ccggcagttg ggtggaatca
     3961 gcggattccc caaaatagaa gaaagtgaat atgatacatt cggggtagga cacgcttcca
     4021 cttccatctc agcagcactc ggcatggctg tggcagccag catgagaggc gaagaccgca
     4081 aagtggtagc tgtgattggc gacggttcca tgaccggcgg acttgccttc gaagggctca
     4141 acaacacttc gatggataaa aacgatatgc tcattattct gaatgacaac cagatggcta
     4201 tcgacccgat taacggcgga ttcacccagt acctggtgga tatcaccact tccaaaacct
     4261 acaacaaaat acgtcataaa gtatataatc tcttcagaaa aagcagactg gtagacgacg
     4321 aaaaaaagaa caagattatc cggttcaaca acagtctgaa aactgctttc acacaaaaag
     4381 accacaacat cttcgaagga ctgaatatcc gctatttcgg tccggtggac gggcacgatg
     4441 taagtaatct cattcgcatt ctggcagaaa ttaaagaatt caaaggtccg aaagtgctgc
     4501 atgtaatcac tcaaaaaggt aaaggattcg aaccggccga aaaatcggca accgtgtggc
     4561 atgcacccgg aaagttcgac cccgaaaccg gtgagcggct tgtgtgcaat cacaacaacg
     4621 ccgaaccacc cctctttcag gatgtttttg gaaaaacatt gctcgaactg gccaagcaaa
     4681 acgataaaat catcggtatt acccccgcca tgccttcagg ctgctccatg accatcatgc
     4741 agcggcgttt ccccgagcgt gttttcgatg tgggaatagc cgaggcgcac gccgtaactt
     4801 tctccgccgg actggccaaa gaaggaatgg tacccttctg caatatatat tcatcgttta
     4861 tgcagcgggc gtacgatagc gtcatccacg atgtggccat ccagaaactg cctgtcatta
     4921 tgtgtctgga ccgcgccggc attgtaggct ccgatggagc tacccaccag gggcagtttg
     4981 acctggctta catgcgttgt attccgaacc tgactatagc agctccgcgc aacgaaatgg
     5041 aactccgaaa ccttatgtac acggctcaac tgcccggtaa gggtcctttc gtgatacgat
     5101 accctcgtgg aaaaggtttt aacgtcaact ggcacaatga gtggcgcgaa ctgaccaccg
     5161 gcaggggtga atgtctgaaa accggaaccg acctggcagt gataaccatt ggaacaatgg
     5221 ctgccgttgc cgaaaaagcc atcaattctg tagaaaatct gaccggtaaa agcattgccc
     5281 actacgatgc ccgcttcctc aagccgcttg acgaagaaat gctacgtgaa atcggagaaa
     5341 aattcaccaa agtaattacc atcgaagacg gaaccatcga aggaggatta ggctcggctg
     5401 tgcttgaatt catgtccgac atcaactatc accccgaagt gctccggata ggtattccgg
     5461 atgtattcgt agagcacggt acacccgaag agttgcatgc catgctcgga atgaatgcag
     5521 aaggcgtcga gaagaaaata aaggacatga tagacgcaaa agtataaggg atcgtaccgg
     5581 tatcttctga tttaacagtt aaaaacgtct tacaaaacat tcaacaaact aaatactcaa
     5641 tagaaatgaa actaaaaacc accctgctga ttttcagctt cgttcttacc cacacattat
     5701 tgtcgcaaaa tcagagtcag cttgcaccca cacctccgat gggattcatg acctggaatt
     5761 attttgggct cgatattcac gaaaacgaca taaaaacact ggcagatgcc atggtggaaa
     5821 ccggactgag agacctcgga tataactata tttttattga cgacggctgg cagggaggca
     5881 gagataacaa aaataacatc attgctgatc ctgcaaagtt cccctccgga atgaaatcac
     5941 tcgtagatta tgttcatacc aaaggtatga aaataggtat ctactcggat gctgctccgc
     6001 ttacctgcgg gggctacacg gcaagtctta atttcgagga acaggatgcc aaaacatttg
     6061 ccgcatgggg atttgattac ctgaagtatg actattgcgg cgctccggcc gactggcaga
     6121 cagccatatc acgctacgag cggatggcaa aagcccttca aaacagcgga cgtgacattg
     6181 cattcggtat ctgtgaatgg ggcgacagat cgccgtggct atgggcacgc aaagccggag
     6241 gacacctctg gcgaactacc gccgatgtac gtgataaatg gaaaagccat gcaccggcgg
     6301 gtagtgcacc tcacgaactt cacgggtatg gagcaggaat tctggatatt ctcgaaatca
     6361 atgccggact cgacaaatat gccggaccga acggatggaa cgatccggat atgctcgtag
     6421 tgggacttta cggcaaaaaa ggagcaccat ctaccggttt gggaggtaca ggctgcaccg
     6481 acaccgaata tcagtcgcag atgtcgctct ggtgcctcat ggccgcaccg cttatgatca
     6541 cctgcgatgt gcgcaatatg aatgaagcta caaaacgtat tctgaccaat aaagatatca
     6601 ttgctattga ccaggatccg ctcggaattc aggcagagcg aaaactgaaa accgacctgt
     6661 tgcagatatt tgtaaaacca ctttcaaacg gcgatgtagc attgggaata ctgaacacat
     6721 ccgatcagga acaaacgata gaggttaatc ccgaaacgct gggtattttc aacaaaagga
     6781 aagcaaaaga cctctggagc ggccaaacga tgaaaacggg caaacgtatc aaagtaaaga
     6841 tggctgcaca cgaaacgaaa gtttttcgcc tgtccgaata acagggtgag ttgaacataa
     6901 aatgctgaat acagggttct gaaaactcaa agctgacaat aaactgaaat agaattactt
     6961 ttttttgaaa aaaagataca tcaatgaaaa taatcattgc gggagccggt gatgtgggta
     7021 ctcacctggc aaaactcctg gcgaaagaga attttgatac cactttgctg gatgaagatc
     7081 ccaaccgact gacggatctg gagatgaatt acgatttact cacccgcacc ggatcgccca
     7141 cctccatcga aagtctgaaa gaatgtggtg tggaaaaggc ggatttgttt attgccgtca
     7201 ctccctacga aagtataaac atgacttcgt gtatgctggc aaccaatctg ggagccaaac
     7261 gtacacttgc ccgcattgaa aactacgaat acctgatacc cgagaacaaa gaattctttg
     7321 ccaagctggg agttgattac ctcatcctcc ccgagaaact cgcagcagag gaaatcgtgg
     7381 agtcgataaa gtacacctgg ttgaggcaaa atctgagttt tcacaacgaa gcgctgttcc
     7441 tgctcgctgt aaaactacgt gaaaatgcac ttattcttaa taaaaaattt aacaccggtt
     7501 ttttcgatca tggcaaattc cgtgtggtag ccatcaaccg aaacggacat accatcatcc
     7561 cgaatggcgg tgatgaactt atggccaacg atgtggtgta tttcgtaacc atgaaagagc
     7621 acatccagtt tgtgaaagaa caggcaggaa aaaaagacta ccaaatcagg aatgtgatga
     7681 tcatgggcgg aagccgcatt gcacagaaaa ccatccagta ttttccgggc aacatcaacg
     7741 taaaagtaat ggaacgcgac aaggaaaaat gctacctgct cgctggatgt ctgcataatg
     7801 ccctcataat taatgccgac ggtagaaatg tggaggccct gaaatccgaa ggaattgaag
     7861 aaatagatgc tttcatagcc actacctcca attcggaggc caacattctt gcctgcctgg
     7921 aagccaaacg cctgggagtg aaaaaaactg ttgccgaagt cgaaaacatc gattacatac
     7981 aactggccga agaactggat atcggcagcg tgataaacaa aaagatgata gcagccagtt
     8041 acatctatca gcttacgctt gatgccgatg tactcgatgt gaagagcctt acttccgccg
     8101 atgccgaagt ggtggaattt gtagctaagg cgggctccaa aatcacaaaa tcacaaatca
     8161 aaaatattcg ccttccggaa aatgtaaata tcggaggaat cgtgcgcgat ggagtgggta
     8221 gcatagctta cggaaacaca caaattcttc cgggcgatca tgtgatggtg ttctgtgtat
     8281 cttcagccat ccgaaaagtc gaatcaatgt ttaattagtt tcccggggta aaacatttta
     8341 cggttggttg cacctgtgta tgaacagaga cttgaacatt aaactcatta gtagggtgct
     8401 gggcggattg ctgctgattg aagccgcttt tctgcttact cccatagcgg ttgcgctcat
     8461 gtatggcgaa acagaaatga ttcgctacta tctctttacg atgctcatag cacttgtgct
     8521 gggcttaagc ggcattgccg ccgggtggaa agccgaatcg acagtgggca agcgcgaagg
     8581 ggcggtgatc gtgacttcta cctggctgct ttatacgcta attggctctc ttccgttctg
     8641 gatgagtggt ttcatccctg attttacaga tgcctttttt gaaaccttgt cgggcttcac
     8701 cactaccggc acgtctattc tgaacaatat agaatcgctg ccatacaacc tgcttttctg
     8761 gcgttcgatg actcactgga ttggaggtct cggaattgta gtgatttttc tggccatact
     8821 gcccatgatt ggaggtgcag gctatcagct ttttacatcc gaaagtacta cacctacccg
     8881 ggaaaagatt ttgccgaaga tcaaccagac tgccaaaatc cttttgtatg tttatgtggt
     8941 gcttacctta ctctgcgccc ttgcactgct gctggccggt atgaacggat ttgacgccct
     9001 gaatcatgca ctgtccacca tcagcaccgg aggattttct accaaacagc tgagcatagc
     9061 ctattttcaa tcgcctgcca ttgagtatgt cattattttc tttatgtttt tttcgggagt
     9121 aaatttcacg ctttactatt tcctggttaa attcaatttt gataagatta taaaaaacga
     9181 agaacttaaa tactacatca tcgtattggt ggtggcggca gtgattattt tcttgtcaca
     9241 catcgattac aacaattcgt ccggttttcg cgattttgag aagtcgttca gaagcagtct
     9301 ttttattgtc acttcctgta tcacgtccac cgggctctgt accgaagatt acaccctttg
     9361 gaattcgttt acatgggtgg tgatgctggt gttgatgatt tcgggagcct ccacctattc
     9421 cacctccgga ggtatcaaga tttcgcgtgt ggtgcttatt gtcaaattct gttactacga
     9481 atttaaaaaa ctggttcatc cgaatgccat tatacccgta aaattcaatg gccagatagt
     9541 gaaagatgaa atgatgatgc gaattcttgc atttacaatg atatatacat taattattgt
     9601 catcggaact tttgttttga gtttgtcggg attgggcttt attgaatcaa tgagcggaat
     9661 gattacctgt atgagtgatg tggggctggg attggggcag ctcggtccaa ctcacagttt
     9721 ctccgaaata cccgttttca gcaagtggtt ccttgctttt gttatgattg taggacgtct
     9781 ggaggtttat tctgtattat tattgtttac ccctgaattt tggaaaaaat aattatgcca
     9841 ttcaaatcag aaataaactt caaactggtt tcccggttaa taggaaatct gctgctcgtg
     9901 gagagtgcaa ccatgttgtt cgtgctggtt attacactgc tttttaatga agatgatggc
     9961 aggtatttta tttacagttc attgattgct ttggcgttag ccttgctgat gattttattc
    10021 ggacgtaacg cacctgccag agctggcaaa agagaaggtt ccatgattgt aacatttgcc
    10081 tgggtattct tttcggctat cggagcattg cctttctgga tgagcggggc catcccttct
    10141 tacaccaatg cctttttcga aaccatgtca ggctttacca ctaccggagc atcaattctg
    10201 gataatatag aagagttgtc ccacggcatt cttttctggc gtgctatgac ccactggctg
    10261 ggtggattgg gtattgtagt catttcaatg gctattcttc ccatgctcgg tatcaacggt
    10321 gtgcatcttt ttgcagccga aaccaccgga ccaaccaaag ataaattcag cccgaaagtt
    10381 aatgacacag cacgtatatt gtttattgta tatatatttc ttaccattgc cgaaaccgta
    10441 tttctgtatt tcggtggtat gtcgttgttc gattcggtta ctcattcttt ttctacggtg
    10501 gccacaggcg gcttctcaac caaacaaaca agtattgcct actgggagaa ttcaccctat
    10561 attcaatatg tgcttgcttt tttcatgttg ttgtccggcg tcaactttgc catgtatttt
    10621 ttcggctaca aaagaaaatt cttcaaaatc aaagagaatg aagagttgcg gtattatctg
    10681 tatattatga tcggtgcaag tctgatggtt atgatatcgc tcattgattt ttcagatacc
    10741 atcaacctgt ctgtcattga aaaagcatgg cgcgattcat tttttaccat atcgtcactc
    10801 atgactacca caggctttgt tactgtcgac tatatgacct ggaaacccgt cacgtgggta
    10861 gtcattgtca tcattatgct tataggtgca tctgcaggct caacagcagg tggcatcaaa
    10921 gtcgtacgaa tagtcattgc tgccaaggca tgctactatg agttcaaacg cataattcac
    10981 ccatctgcag tcatacctgt gaggtataat aaaatagtgg tacatgataa cacgatgata
    11041 agggtgctca cttttattat actatacctt tcagttacac ttatcggaat actggttctg
    11101 atgatttccg gcatgggaat aagtgagtct atcggaggga tgatcacttg tataagctgt
    11161 gtgggtcccg gattcgaaac ggtaggccca tcgggaaact tcaataatat tcctgaattc
    11221 agcaagtggt ttctctcttt tgtcatgtta atgggcagat tggaattata caccgtcctg
    11281 atgattctta cccctgcttt ctggcgtcat taattttttt atactccgac tatcaccata
    11341 tagaagattc atccggttat tatccgcttt cttctgtctg tttccttttt tttacaggta
    11401 aaaaaatctg ctttttcgta cttttttagc aatagttcat tgttaacgta gctgtatttc
    11461 gtcggtaaac aataaaatca gacgcattaa cttaccaaac aggtagtgtt ttcagcaaaa
    11521 atattacttt ggtgtctgtt ttattcaaat ttttttcatt cttaactggt ctgtttatca
    11581 gttactttaa ttatttcttt gattttcttt gatattttct tttgatttta ttttgttgtt
    11641 ataaaaatag tc
//
LOCUS       DAORKP010000068        11577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_586_length_11577_cov_8.395678, whole genome shotgun sequence.
ACCESSION   DAORKP010000068 DAORKP010000000
VERSION     DAORKP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_11577_cov_8.395678"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLN75_05260"
     CDS             complement(<1..191)
                     /locus_tag="PLN75_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03560.1"
                     /translation="MIYFFQTPDQQIFAVQSTSEFNSETINKLTWLFGEAKLLDNEII
                     QGNFIGPRREMITPWSTNAV"
     gene            419..1192
                     /locus_tag="PLN75_05265"
     CDS             419..1192
                     /locus_tag="PLN75_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRG03561.1"
                     /translation="MIKAVFFDIDGTLVSFETHQVPASARRAVEELRAKGINVFIATG
                     RHKSAINNLGDMQFDGFVTINGGYCMLGDGTCIYRNSIPRSDMEALLNYQQIDTFPCV
                     TMDDNEMYINFHNQNVEKVLEMLAFPKLRVRPLEETLQYDVLQVIAFFEKEQESDIMA
                     ALPGCDSTRWFPLFTDIVPRGSNKSVGIDKMLAHFNILPEETIAFGDGGNDMAMLQHV
                     GLGIAMGNAEDAVKSCAGFVTDTVDADGVYKALKKFELL"
     gene            complement(1197..3392)
                     /locus_tag="PLN75_05270"
     CDS             complement(1197..3392)
                     /locus_tag="PLN75_05270"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRG03562.1"
                     /translation="MNLFTKIFLIWSACMITLTPLHSERPKVGVVLMGGGAKGFAHIG
                     ALKVIEEAGIPIDYIAGTSMGAIVGGLYSIGYDSHDLDSLSKNQDWMHLLTDEIYRYN
                     LYGSEKDKAQTYIVTMNYEKQGIKLPSSIVSGQNIYNLMLDVTTGYHESVDFHRLPIP
                     FSCVAADVKTGKEVVLSDGILPQALRASMAIPGFFSPVKIDSMLLIDGGILNNYPVDV
                     VKKMGADIVIGVSFDKDEKKIEKNMGSFLELTNDFGNFLGNEKLEENLRNTDLSIRVK
                     LDKYNIASFRSDEVDSIIRIGETTTRNKWNELIELKNKLGSDDEISIRQIKNPYIGTD
                     TLLIRSVYIEGLSAEDQQLVLNRLRIGPKTTRKELQNIISRMYGTDLFSKVFYRLENQ
                     KPPYDLVFEIEKKDIRNLNVGIRFDTQDLASILLNSTIRLRTSLNSTFEVTARLNKNP
                     YLQINYSLSSTMLYKGGVQFKTSKNDINIYDKGRLAYNLGFYQNTLNVNFSEFQFYNM
                     NLRLGMDIDYFYYLYELKNSGLPDLNLESKPYMNYFLSGTYDNLNSSYYPTRGFYFNF
                     RYAVKTDNFYRFEGHKPMQSIKFTFIKPHAINKKMTITPLIAGRIILSDTVPFIYSNL
                     AGGQWDSHYLNQQISIQGLQGMEIFEKSLIKTELVLKYNFYKQHSVQATINCTMHDEN
                     IRQLPDRKPILGGTLGYSYNTIIGPLIVRFSYSDRTRKIYPFISAGYYF"
     gene            complement(3508..4455)
                     /locus_tag="PLN75_05275"
     CDS             complement(3508..4455)
                     /locus_tag="PLN75_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRG03563.1"
                     /translation="MSEEIITSNHWLVITNPTSGKRKFQEQGKYVLSELDKAHIPYIY
                     KLTEYSGHTIEIAKSYARRGCLNYLVLGGDGSVSEVINGIFAAEPDDTSKIKIALIPR
                     GTGNDWGRFWGLRKNNKESIKIFRKGNCKLIDIGKIDFIEDNNQSESHYFINSVGFGL
                     DAEVVEITHRMKKYIGSFSLLYTLALLLAVFRYKPTPTQLTINGKSREVKLFTMNIAN
                     GPFSGGGIKQNPYALPYDGTFDMIVVEKPTVRDILTALPLIFNGKLTQHPVIQSFQAK
                     EIWIKTKNEILIEADGIIVPDGHNSRVSIIPEAIQMIVP"
     gene            complement(4547..4849)
                     /locus_tag="PLN75_05280"
                     /pseudo
     CDS             complement(4547..4849)
                     /locus_tag="PLN75_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(4860..5921)
                     /gene="aroC"
                     /locus_tag="PLN75_05285"
     CDS             complement(4860..5921)
                     /gene="aroC"
                     /locus_tag="PLN75_05285"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRG03564.1"
                     /translation="MNTTGKLFTFTSFGESHGKGIGGIIDGCPAGIELDETFIQNELS
                     RRRPGQSIISTPRSEEDNVEFLSGIFEGKTTGTPIAFVIWNKNQESKDYDHLKDVYRP
                     SHADFTYQQKYGIRDHRGGGRSSARATAAWVAAGAIAKLVLNQKGVEIKAYTSQVGHI
                     SVGKSYENLDLSLIESNIVRCPEPETAEKMIAYIDELRTEGNSIGGIVSCVVKGVPAG
                     WGEPVFDKLHARLANAMMNINAVHGFDYGSGFEGVRLTGAEMNDAFTLKGENVTTKTN
                     NSGGIQGGISNGQDIYFRVLFKPVATISKKQETLDKQLNKIELEARGRHDPCVLPRAV
                     PIVEAMAALTLVDLYLISK"
     gene            complement(6142..7536)
                     /gene="glmM"
                     /locus_tag="PLN75_05290"
     CDS             complement(6142..7536)
                     /gene="glmM"
                     /locus_tag="PLN75_05290"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRG03565.1"
                     /translation="MALVKSISGIRGTIGGRAGEGLSPLDVVKFTSAYAAFIKSGGKS
                     DSRTIVVGRDARISGEMVNKLVVGTLLGMGMNVTDIGLATTPTTELAVTMEKAAGGII
                     LTASHNPKQWNALKLLNEKGEFLNAKEGETILKLAEAEDFTFTDVDNIGKLTVKDFYT
                     QKHIESVLALQLVDVEAIKAANFRVAIDCVNSVGGIAIPELLEALGVKTLEKLYCEPN
                     GHFPHNPEPLPEHLTDISDVMKKGKFDVGFVVDPDVDRLAIIAEDGSMFGEEYTLVSI
                     ADYILRHTPGNTVSNLSSTRALRDVTRKYNYDYNAAAVGEVNVVAKMKETNAVIGGEG
                     NGGVIYPASHYGRDALVGIALFLTHLAKEKKTVSELRKTYPNYFMSKNKIELTPNIDV
                     DGVLAVIKGSYKQYEVNDIDGVKIDFPQGWVHLRKSNTEPIIRIYSEAGTPEQADNFA
                     KAIMEEIKRIANIA"
     gene            complement(7599..8141)
                     /locus_tag="PLN75_05295"
     CDS             complement(7599..8141)
                     /locus_tag="PLN75_05295"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HRG03566.1"
                     /translation="MKKILTAFFSLFFIIVFAACDNTKSYSELQDEEIVTIENYIKKN
                     NIQVVTTKPADNAWGTNVYYKTASGLYIHIVNTGDKTDSLVVNSTVGYRVIEYLLDEK
                     KTIQFKNWEVRDYNNPIQFTYGSSTAISSIGTGMHEAIGFMKYKNSEAKVIVPSGLNI
                     STYNQNVIPVGSDIKITVIN"
     gene            complement(8271..8711)
                     /locus_tag="PLN75_05300"
     CDS             complement(8271..8711)
                     /locus_tag="PLN75_05300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRG03567.1"
                     /translation="MPANKTLETDSTEIKLLELNKRITQLEDSAIVAFLALQPGSYTK
                     ANAGFWYHIQQTGGSGYVVDDSVAVFSYTAFTLEGTQVMHEELKTIHFGKKEAVTGLE
                     EGLKLLKHGGKARFIVPWYLAYGSLGSGKIKPYTTLIYEVEVLE"
     gene            complement(8914..11358)
                     /gene="thrA"
                     /locus_tag="PLN75_05305"
     CDS             complement(8914..11358)
                     /gene="thrA"
                     /locus_tag="PLN75_05305"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRG03568.1"
                     /translation="MKVIKFGGSSVGSIEGILSVKKIAESQQESVVVIVSALSGITDQ
                     LYATSNMASQGNENYLTAYEEMVSRHYQLVEEVVPESRKTKTIQKLNEYFSDLTNIFR
                     GIYLIKDISTRITDTIVSYGEVLSSVIVSNLIENATHYDARNFIKTVNKFDKHIVDFP
                     ITNKLIADTFRNVTGISVCGGFISTDVSTGYITNLGRGGSDYTASIIASALNASKLEI
                     WTDVDGFMTADPKIISNAYVIEHLSFSEAMELSNFGAKVIYPPTIFPVYHKNIPVEIK
                     NTFNPDTPGTLISSEKKPNKNGETKAIKGISSINDTSLITIQGLGMVGVIGVNYRIFR
                     ALAKNGISVFMVSQASSENTTSIGVRNADSRMAVDVLREEFKTEIQQGEINQIFSEDD
                     LVTVAIVGDNMKRTPGIAGKLFGTLGRNGINVIACAQGASETNISFVTDRAVLRKTLN
                     VIHDSFFLSEYKVLNLFIVGVGTVGSSLLNQIKSQRHKLMDQNGLKINVAGIASSSKM
                     MFSRAGIDLENCVATLRAEGEECNTKTIRDEILKMNIFNSVFVDCTASQEVSDIYQSL
                     LENNISVVTANKIAASSEYEKYMKLKDTSRLRGVKFLFETNVGAGLPIINTMNNLTNS
                     GDHILKMEAVLSGTLNFIFNTISEKVTLSKAIRMAKESGFSEPDPRIDLSGKDVIRKL
                     VILAREAGYKVEQENVKKNLFIPDRYFEGSLDDFWKKIPELDEKFEKDRKELSTAGKY
                     WRFVATMDRGECSVGLQTIDSIHPFYDLQGSNNIVRITTERYNEYPLIIKGYGAGASV
                     TAAGVFADIISIANIR"
     gene            11502..11575
                     /locus_tag="PLN75_05310"
     tRNA            11502..11575
                     /locus_tag="PLN75_05310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11536..11538,aa:Arg,seq:tct)
BASE COUNT         3034 a         2383 c         2450 g         3710 t
ORIGIN      
        1 accgcattgg tactccatgg ggtaatcatt tcacggcgcg gaccaataaa attaccctga
       61 attatttcat tgtcaagtag tttagcttcg ccaaatagcc aggttagttt gtttattgtt
      121 tcgctgttga attctgaagt ggattgtact gcgaaaattt gctgatcggg agtttgaaaa
      181 aagtaaatca tgcggatgaa tgctaaggat taaactcaat ttagtgattt ttatcagagc
      241 acaaaggtac taattaaatg gcacgctgaa aagtaaaaaa acgattttta agtgattgca
      301 atcaattcat agtcaatcta aaaaaactca tagatttgtt gttgccgtcg tttttgacta
      361 attttgacat ccaaaaagga atttggtttt attctgattt caatacatgg ttaactaaat
      421 gataaaagct gttttttttg atattgatgg tacgcttgta agtttcgaaa ctcatcaggt
      481 tccggcaagt gcacgcaggg ctgtagagga attgagagct aaaggtatta acgtgtttat
      541 tgctaccgga cgtcataaat cggccatcaa caatcttggc gatatgcagt tcgatggatt
      601 tgtaaccatc aacggcggct actgcatgct cggtgacggt acgtgcattt atcgcaacag
      661 tattccccgt tcagacatgg aagctttact caactatcag caaattgata cttttccctg
      721 tgtgacgatg gacgacaatg aaatgtacat caactttcac aaccaaaacg tggaaaaggt
      781 actggaaatg cttgcatttc caaaattgcg ggttcgtcct ctcgaagaaa cattgcaata
      841 tgatgttttg caggtcattg cattttttga aaaagaacag gaaagtgata taatggcagc
      901 gctgcccgga tgtgattcca ccaggtggtt tccactgttt accgatatag ttccccgggg
      961 cagtaataaa agtgtaggga ttgacaaaat gcttgcacat tttaatattt tacccgaaga
     1021 aacaatcgct tttggtgatg gcggaaacga tatggctatg ttgcagcatg tggggttggg
     1081 tatagctatg ggtaatgctg aagatgccgt taagtcgtgt gccggttttg taaccgatac
     1141 ggttgatgct gacggtgtgt ataaagccct gaaaaagttt gaattgcttt gaagtatcaa
     1201 aaatagtatc ctgcactgat gaagggataa atctttcggg tgcggtcaga atagctgaat
     1261 cgtactatca gaggccctat aattgtattg tatgaatatc ccagtgtacc tcccagaata
     1321 ggttttctgt caggcagctg ccgtatattt tcatcgtgca tggtgcagtt aatggttgct
     1381 tgtaccgaat gttgtttata gaaattatac ttcaacacca gttcggtttt tatcagcgat
     1441 ttttcaaaaa tttccattcc ctgtaatccc tgaatcgaaa tttgctgatt gagataatga
     1501 ctatcccact gaccacccgc gagattggaa tatataaagg gaacggtgtc gctcaggata
     1561 atccggcctg ctataagagg tgtaatagtc atttttttgt tgatggcatg gggttttatg
     1621 aacgtaaact taatcgattg catgggttta tgaccttcaa agcggtaaaa attatcggtt
     1681 ttcactgcgt acctgaagtt gaaataaaac ccgcgggtag ggtagtagct gctgttcagg
     1741 ttgtcgtaag ttccgctcag aaaataattc atatacggct ttgattccag attcaaatca
     1801 ggtagtcccg aattcttgag ttcgtacaga tagtagaaat aatcaatgtc catgcccaga
     1861 cgtaaattca tattgtagaa ttgaaattcg ctaaagttca cattcagggt attctgataa
     1921 aatcccaggt tgtaggccaa tctgcctttg tcgtaaatgt tgatgtcgtt tttggatgtt
     1981 ttgaattgta ctccaccttt gtataacatc gtactactca gcgaataatt tatctgcaga
     2041 tacggatttt tattgagccg ggcagtcact tcaaatgtag aattgagtga agttctcaat
     2101 ctgatggtgc tgttgagtag tattgaagcc agatcttgcg tgtcaaagcg aatgcctacg
     2161 ttgagattgc ggatgtcttt cttttcgatt tcgaatacca gatcgtacgg aggtttttga
     2221 ttttcgagtc tgtaaaaaac tttcgaaaat aaatcagtgc catacattcg cgaaatgatg
     2281 ttttgcaact cttttcgcgt ggttttgggg ccaatgcgca gacgattcag caccagttgc
     2341 tgatcttcgg ccgataaacc ttcgatataa acgcttctga tcaacagagt gtctgtcccg
     2401 atgtatggat tttttatctg tcggattgaa atttcatcgt ccgatcccag tttattcttc
     2461 agttcaatca gctcattcca cttatttcgg gtcgttgttt ctccaatccg gataatgctg
     2521 tcaacttcat ccgaccgaaa actggctata ttgtatttat ctagtttcac ccgaatggaa
     2581 agatcagtat tccgaaggtt ttcctccagc ttttcgttac ccagaaaatt gccgaaatca
     2641 tttgtcagtt cgaggaatga acccatattt ttttctattt ttttctcatc cttatcgaaa
     2701 ctgacaccga ttacaatatc agctcccatc ttctttacca catccacagg atagttgttg
     2761 agaattcctc catcaataag tagcatactg tcaatcttaa caggactgaa aaatcctgga
     2821 atggccatac tggctcgcaa agcttgcgga agaatcccgt ctgagagaac aacttccttt
     2881 cccgttttga catctgctgc cacgcagctg aaaggaatcg gtagtcggtg aaaatctacg
     2941 ctttcgtgat atccggtggt tacatcaagc atcaggttgt aaatattctg accgctcaca
     3001 atacttgaag gcagcttaat gccctgtttt tcgtagttca tcgtcacgat gtaggtttgt
     3061 gccttatctt tttcagatcc gtacaagttg tatctgtata tttcgtcggt aagtaaatgc
     3121 atccagtcct gatttttcga gagcgaatca agatcatgtg aatcgtatcc gatgctgtaa
     3181 agtccgccta caatagctcc catacttgtt ccggctatat aatcaatcgg tattccggct
     3241 tcttcgatga ctttcagggc gccaatatgg gcaaatcctt tagcgccgcc acccatcagt
     3301 accacaccaa ctttcggacg ctcgctgtga agcggggtaa gtgtgatcat gcaagccgac
     3361 caaatcagga atattttggt aaagagattc atcttttaag aaataatttg aacagaatct
     3421 gactaatatt ataactatgg cagcgacgaa ttgttttgta aaagatggtg agtgcagttg
     3481 atttaatgaa acagaagaga taaatttcta cggaacaatc atctgtattg cttcaggaat
     3541 aatggaaaca cggctgttat gcccatcggg gacaattatc ccatctgctt caatcagaat
     3601 ctcatttttg gtttttatcc aaatttcctt tgcttgaaaa ctctgaataa cgggatgctg
     3661 agtgagtttg ccgttgaaaa tgagcggaag agctgtaagt atatcacgta cggtgggttt
     3721 ttccactaca atcatatcaa aggtaccatc gtaggggagt gcatagggat tttgtttgat
     3781 gcctccaccg ctgaatggtc cgttggcaat gttcatggtg aaaagtttta cttctcgtga
     3841 cttaccattg attgtcagtt gcgtaggagt aggtttgtat ctgaaaacgg cgagcagtaa
     3901 tgccagcgtg tacaacaggg agaaacttcc gatatatttt ttcatccggt gagttatttc
     3961 cacaacttct gcatcgagcc cgaaacctac cgaattgatg aaataatgac tttcggattg
     4021 attgttgtct tcgatgaaat caatttttcc gatatcaatc agcttgcaat tgccttttcg
     4081 gaaaatttta attgattctt tgttgttttt tcttaatccc cagaatcttc cccaatcatt
     4141 tccggtacct cgcggtatga gtgctatttt tattttcgat gtatcatccg gctcggctgc
     4201 gaatatacca tttatgactt cacttaccga cccgtcgccg cctaatacaa gataattcag
     4261 acatccgcgc ctggcatacg attttgctat ttcaatggta tgtccggagt attcggttag
     4321 tttgtaaata tacggaatgt gtgctttgtc gagttccgaa agtacatatt tcccctgttc
     4381 ctgaaatttt ctttttccgg aagttgggtt ggtgattacc agccaatgat tggatgtgat
     4441 gatttcttcg gacatatttg cccctccaac ctccccgaat gggaggcttt atgagttatt
     4501 gtttttgttt aaaagttttt tgtacccgat ttcgaggttt ttttttattg actttgatag
     4561 caatatcact gtttagaacg cctgaaagca tggcaatgcc ttgttctcta aaagcaaacg
     4621 tgagataact ccccaacctg aggtgaaatt ttggtacctc aaattttcca attcaataga
     4681 tgtcaattca aacataaaat cagccgctat gcgtttaata ttacgtctta cagcacgttt
     4741 taacgctttt tttttcaatc tggtacattt cagccaaatc aaaatcaagc attactttta
     4801 caccccgaat ctcaaagatt ttgttttgaa taagttgaat atcagtcatt tctttcagtt
     4861 tatttactta tcaggtacaa atccaccaac gtaagcgcag ccatggcttc cacaatgggt
     4921 actgcacggg gcagcacaca tggatcatgc cgaccgcggg cttcaagttc tattttattc
     4981 agctgtttat caagcgtttc ttgttttttg ctgatggtgg ctaccggttt gaacaacacg
     5041 cggaaataaa tatcctgacc gttgctgatg cccccctgaa tgccgcctga gttgttggtt
     5101 tttgtagtta cgttttcacc cttcagcgtg aaagcgtcgt tcatttcggc accggtgagg
     5161 cgaacacctt caaaaccgct tccgtaatca aatccgtgaa ctgcattgat gttcatcatc
     5221 gcattggcaa ggcgggcatg cagcttatcg aatacgggtt ctccccaacc tgcagggaca
     5281 cctttcacca cacaactgac aataccgcca atggaatttc cttcagtccg caattcatca
     5341 atgtaggcaa tcattttttc ggccgtttcg ggttccggac aacgaacaat attactctca
     5401 atcaacgata aatccaggtt ttcgtacgat ttacccaccg aaatatgtcc cacctgcgaa
     5461 gtgtatgctt taatttcaac acctttttga tttaatacca gtttggcaat ggctcctgct
     5521 gccacccacg cagccgtagc gcgtgctgaa ctccgtccgc caccccgatg gtcgcgtata
     5581 ccgtatttct gctggtaggt aaagtctgca tgcgatggcc gatagacatc tttcaggtgg
     5641 tcgtaatctt tactttcctg atttttgttc cagatgacaa acgcaatggg tgtgccggtg
     5701 gttttacctt caaaaatacc tgacagaaac tctacattat cttcttcgga gcggggtgtg
     5761 gaaataatgg actgacccgg acgacgacgc gagagttcat tctgaataaa tgtttcatcc
     5821 agttcaattc cggcaggaca tccgtcgata atgccgccga tacctttgcc atgcgattcg
     5881 ccgaaagatg tgaatgtgaa gagtttgcct gtcgtattca tgaacacccg taactttcta
     5941 aaaaaggaaa tttcgttaag tttgctgtaa atttattgca aagttaaaat aaaatttgat
     6001 taaaaataaa tcaattgata tttcaatgct ttaaaagctc acaaattgaa aaaaaaggat
     6061 taaaattcaa ataaaaaacg ccacattaca tggcgtttta tttatttcat ttagtaagac
     6121 ttacagttga attcagtcag tttatgcgat attagcaatt ctcttaattt cttccataat
     6181 tgcttttgca aagttatctg cctgctccgg agtgcctgct tcggagtaaa tgcggataat
     6241 cggttcggtg ttggatttgc gcagatgcac ccagccttgc gggaagtcaa ttttcactcc
     6301 gtcgatatca ttcacttcgt attgtttata gctgccttta attactgcca gtacgccgtc
     6361 cacatcaata ttcggagtga gttcaatttt atttttactc atgaaatagt ttggatacgt
     6421 cttgcggagt tcggaaaccg ttttcttttc tttggctaaa tgggtaagaa aaagagcaat
     6481 acccaccagc gcatcccgtc cgtagtggct cgctggatag attacaccac cgtttccttc
     6541 gccaccgatt acagcattag tttctttcat tttagctact acatttactt cgcctactgc
     6601 tgcagcattg tagtcgtagt tgtattttcg tgtcacatcg cgcagtgcgc gggtggaact
     6661 caggtttgaa accgtattgc cgggtgtatg ccggaggatg taatcggcaa tggatacaag
     6721 cgtatattct tctccaaaca tgctgccgtc ttcggcaata atggccagac ggtctacatc
     6781 gggatctact acaaaaccta catcgaattt tccttttttc atcacatctg aaatatctgt
     6841 caggtgctcg ggcagtggct cgggattgtg cggaaaatgt ccgttcggtt cgcagtaaag
     6901 tttttctaag gttttaacgc cgagtgcttc caataattcg ggtatggcaa ttccgcccac
     6961 agaatttaca cagtcaattg ctacgcggaa attagctgct ttgatggctt ccacgtccac
     7021 cagttgaagt gccaaaacac tttcgatgtg tttttgcgta taaaagtctt ttacggtcag
     7081 tttaccgata ttatccacat ctgtaaatgt aaagtcttcg gcttcagcca gtttcaatat
     7141 ggtttcacct tctttggcat tcagaaattc acctttttcg ttcagcagtt tcagtgcatt
     7201 ccattgtttc ggattgtggc tggcagtaag tataatgcct cctgcagctt tttccatagt
     7261 taccgccaat tcggtggtag gtgttgttgc cagtccgata tcggtaacgt tcattcccat
     7321 tccaagtagc gttcccacta ccagtttgtt aaccatttct cccgagatgc gcgcatcgcg
     7381 tcctacgacg atggtgcgcg agtccgattt tccgcctgat ttgataaatg ctgcgtaggc
     7441 tgatgtgaat tttacaacgt caagcggact taatccttca ccggcacgtc cgccgatggt
     7501 tcctctgatt cctgagattg atttaactaa tgccatagaa atttgtaaat gaagtatgtg
     7561 tatatgaaac caaaaacccc ttatgaaggg gctcttgttt aattaattac ggtaattttg
     7621 atgtcgcttc caaccggaat tacattttga ttgtaggtgc ttatattcaa tccggaggga
     7681 acaatgactt tagcttctga atttttatat ttcataaatc cgattgcctc gtgcatccct
     7741 gtaccgatac tcgaaatggc tgtgctgctg ccgtaggtaa actgaatggg attgttgtaa
     7801 tctctcactt cccagttttt gaactgaatg gttttctttt cgtccagcag gtattcaatg
     7861 actctgtaac ctactgtcga attcacaacc agagaatcgg ttttgtcgcc tgtgttaact
     7921 atgtggatat aaagtcctga tgcggtttta tagtacacgt tggttcccca ggcattgtca
     7981 gccggtttgg tggtaacaac ctgaatgtta tttttcttaa tgtaattttc aatagtgaca
     8041 atttcttcgt cctgaagctc ggagtaactt ttagtattat cgcaagcagc aaatactatg
     8101 atgaaaaaca aggagaaaaa tgcggttaaa atctttttca tttagagatt tattttccta
     8161 atttatagag tttggaattt ggatgcaaat atccgatttt atcgtgatat acaaaagtta
     8221 attttcagta atctggtgtc agagctgtta tttgtgttaa aaaaaatggt ttattcaagt
     8281 acttccactt cgtaaataag ggtggtgtag ggtttaattt ttccgcttcc cagcgagccg
     8341 taagccaggt accagggaac gatgaagcgt gctttcccgc catgtttcag tagttttaat
     8401 ccttcctcga gtccggttac tgcctctttt tttccaaaat gtatagtttt gagttcttcg
     8461 tgcatcactt gtgtaccttc aagtgtgaat gccgtgtagc tgaatacagc tactgaatca
     8521 tcgactacat atccggagcc gccggtttgc tgaatgtgat accagaaacc tgcatttgct
     8581 ttggtgtagc tcccgggctg aagcgccaga aatgccacaa tagcgctgtc ttcgagttgt
     8641 gtgattcttt tattgagttc aaggagtttt atctcagtag aatctgtttc aagagtttta
     8701 ttggctggca attgagctgt ttgtttccgg catgatacat taaggagtaa aaaaattaca
     8761 catccaaaaa tcagaaataa aattgttggt tgacgaaacg aattatgaaa ttttgaaatc
     8821 atgtatttat ttcggtttac aatttatttt gaaaaaaata attttatcta aaacaagttc
     8881 aacgcctggt tgcagaaatt ttagatcatt tttttacctg atattggcaa tgctgatgat
     8941 gtcggcaaaa actccggctg ccgtaacgct ggcaccggct ccgtagcctt tgatgatcag
     9001 cgggtattcg ttgtaacgct cggtggtgat tctcacgatg ttgttgctgc cctgcaaatc
     9061 gtaaaacgga tgtatgctgt cgatggtttg aagtcctacg ctgcattctc ctctgtccat
     9121 ggtggcaacg aagcgccagt atttaccagc tgtactaagt tctttgcggt ctttttcgaa
     9181 tttctcgtcg agttccggaa tttttttcca gaaatcatcc agcgatcctt caaaataccg
     9241 gtcggggata aacagatttt tcttcacatt ttcctgttct accttgtagc cggcttcgcg
     9301 cgccagaata acgagtttgc gaatcacatc ttttccgctt aaatcgatcc gcggatcagg
     9361 ctcggaaaat ccggattctt tagccatccg tatcgccttg cttagcgtaa ctttttcact
     9421 tatggtgttg aaaatgaaat ttaacgtacc tgaaagcacc gcttccattt tcagaatgtg
     9481 atcgccgctg ttggtcaggt tgttcattgt attgataatg ggcaaaccgg ctcctacgtt
     9541 ggtttcaaac aggaatttca caccacgcag tctggaagtg tctttgagtt tcatgtattt
     9601 ttcatactcg gatgaagctg caatcttatt ggcagtaacc accgagatgt tgttttcaag
     9661 caacgactga taaatatccg aaacttcctg actggcagtg cagtcaacga aaacggaatt
     9721 gaatatattc attttcaaaa tttcatcccg tatggttttg gtgttacatt cctcgccttc
     9781 ggctctgagt gtggctacgc aattctcaag atcaatgcct gcgcgcgaaa acatcatttt
     9841 ggagctggaa gcgatgcctg ccacgtttat tttcagtccg ttctgatcca tcagtttgtg
     9901 gcgctgactc ttgatctggt tcagcagact gctgcctacg gttcctacgc ctacgataaa
     9961 caagttgagg actttgtatt ccgagaggaa aaatgagtcg tgaatgacgt tgagtgtttt
    10021 acgaagtacc gccctgtcgg tgacgaaaga tatgttggtc tccgaggctc cctgcgcaca
    10081 ggctattaca ttgataccat tccggcctag tgtaccgaaa agtttaccgg caatgcccgg
    10141 agtgcgtttc atattgtcgc ctacgatggc cacagtgacc agatcgtctt ccgaaaaaat
    10201 ctgattgatt tctccctgct gaatttctgt tttgaattct tcgcgaagta catctactgc
    10261 catgcggctg tcggcgtttc gcaccccgat ggaagtggta ttttccgatg aagcctggga
    10321 aaccatgaaa acactgatgc cgtttttggc cagagcgcgg aaaatgcggt agtttactcc
    10381 gattacacct accataccga gtccctggat ggtgataagg gaagtatcgt tgatagaaga
    10441 aataccttta atggctttgg tttctccgtt cttgttcggt ttcttttcgc tggaaatgag
    10501 cgtacccgga gtgtcgggat tgaaggtgtt ttttatttct acaggtatat ttttatgata
    10561 caccggaaaa atggtgggtg gataaatcac tttggcaccg aagttggata gttccatcgc
    10621 ttcgctgaag ctcaaatgtt cgattacata agcgttgctg atgatttttg gatcggcggt
    10681 cataaaccca tccacgtcgg tccagatttc gagtttggaa gcgttgagtg ccgatgcgat
    10741 gatggaagcc gtatagtccg aaccaccacg acccagattg gtgatatagc ccgtacttac
    10801 atcagtagaa atgaatcctc cgcaaacaga aatgccggta acgtttctaa atgtatcggc
    10861 aatgagcttg ttggttattg gaaaatcaac aatgtgctta tcgaacttat tgacggtttt
    10921 gataaaatta cgtgcatcgt aatgtgtggc attttcaatc aaattgctca caatgactga
    10981 tgaaaggact tcaccgtaac ttacaatggt atcggtaatg cgggtagaaa tatccttaat
    11041 aagataaatg cctctgaaga tgttggtcag atcactgaaa tattcgttca gtttctgtat
    11101 cgtttttgtt tttcttgatt cggggactac ctcttccacc agctgataat ggcgtgatac
    11161 catttcttcg taagctgtga ggtagttttc atttccctgc gaagccatat tggaagtagc
    11221 gtaaagctga tcggtaatgc ccgataatgc cgaaacaatt accacaaccg actcttgctg
    11281 cgattccgca atttttttta cacttaatat tccttcaatc gaaccgaccg atgaaccgcc
    11341 aaattttatt accttcatcc tgaataattg tttgaaaaat ttcaggttgc aatattactc
    11401 attttttggc aaataatgag aaatctgatt gcttttttgg cgataagtgt tgcttagttg
    11461 taaaaaatca cgtacttttg caaaactttt ttttgaaaaa tggtctcgta gctcagctgg
    11521 atagagcaac agccttctaa gctgtgggtc gtgggttcga atcccgccgg gatcaca
//
LOCUS       DAORKP010000069        11302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_629_length_11302_cov_10.194363, whole genome shotgun sequence.
ACCESSION   DAORKP010000069 DAORKP010000000
VERSION     DAORKP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_11302_cov_10.194363"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..742)
                     /locus_tag="PLN75_05315"
     CDS             complement(137..742)
                     /locus_tag="PLN75_05315"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRG03569.1"
                     /translation="MTNINLENTDNRELRSTTPPSGGWGAGDASDIIKASIRNIPDFP
                     IPGIQFKDITTALKNPEILKLMTDTLYEYYKNKGITKVVGVESRGFVIGSILAYKLNA
                     GFVLLRKPGKLPAETFSLKYELEYGVDGLEIHKDAIEPDDVILLHDDLLATGGSANAS
                     LQLLRKFGDNPIYVSFLIELLELEGITRLDEAMDVYSLVQF"
     gene            complement(871..>1743)
                     /locus_tag="PLN75_05320"
     CDS             complement(871..>1743)
                     /locus_tag="PLN75_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HRG03570.1"
                     /translation="YKQLGENKTLLMVFFNSSLRTRLSTQKAGMNLGMNTMVLDINQG
                     AWKLETERGVIMDGDKPEHILEAIPVMGTYCDIIGVRAFAQFEDKEFDYQETILNQFI
                     HYSGKPVFSMEAATGHPLQAFADLITIEEYKRTARPKVVLTWAPHPKALPQAVPNSFA
                     DFMNEADVDFVITHPEGYELSEKFVRDARVEYDQMKAFEGADFVYAKNWAAYQDPNYG
                     KILSTDRSWTVSERQMAVTNNAYFMHCLPVRRNMIVTDDVIESPQSIVIPEAANREIS
                     AQYVIKKMLESTAP"
     assembly_gap    1744..1843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1913..3472)
                     /locus_tag="PLN75_05325"
     CDS             complement(1913..3472)
                     /locus_tag="PLN75_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HRG03571.1"
                     /translation="MVNKKYMNRILWADDEIDLLRPYVIFLKEKGYSVTTVTNGKDAI
                     DLCQKESFDIIFLDENMPGLSGLETLTQIKTIDPNVPLVMITKSEEEDIMNMAIGNKI
                     ADYLTKPVNPSQILLTLKKNIHKREIVSEHATSTYQQEFRNIGMQISDSLTWENWVEV
                     YKKLVFWELELAEADNNMDEMLRMQKDEANSTFNKFIKKNYMDWMVNPNGRPMISPEL
                     MKNKVFPLLDEGEKVFLVVVDNFRYDQWLVIREVLNEFYTFTEEETYCSILPTATQYA
                     RNAIFSGLMPLQIQQMFPDLWVEEEDEEGKNIHEEALLKTQFDRFRKKYSFSFNKVFE
                     SSYCQKVIDQLPRMDGNNLNVVVLNFIDMLSHARTESKMMRELANDEKAYRSLTLSWF
                     RHSATYDLFKAIAHRGYKVIVTTDHGTIQVNNAIKVVGDKNVNTNLRYKVGKNLSYNK
                     KEVFEILQPHKAGLPSPNVSSAYIFAGNDDFFAYPNNYNYYVSYYKNTFQHGGISLEE
                     MIIPVVTMEPK"
     gene            complement(3663..7121)
                     /locus_tag="PLN75_05330"
     CDS             complement(3663..7121)
                     /locus_tag="PLN75_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03572.1"
                     /translation="MLTRFNIAFNGNTSYNEGMENIAKANKDDYSQIINMFPVSNHSN
                     TSGAAGNMNRTIEKSRKAIKLHSIKKKPERDYKRYNDPDYQAFYNQNEFNPELKNAWM
                     QLGKAEFHKGDFLGSVGTFSYITRFYATDPDLVMAAQLWIARAYAEMDWIYEAEDVIE
                     KIKPNQLKGDNIGLYTSTNADLLIKKNQYPEAIPFLKTAIKEEKNKKLRNRFKFVLAQ
                     LLEKTGNKKEAENYYTQVIKSTPPYEMDFNARINLAQVKTANPAGMERELKKMARNRN
                     NKDYLDQIYTALGNIYIQKKDSAKAIENYNLAIGKSTRNGVEKGVALVILGNLYYQQR
                     DYVKAHPYYDEAAKIYTKEFDDYEKISHRAEVLGELVKEYETVHLQDSLQALSVMPEK
                     ERLLAINRVIEKIKEDEKAEAEGKLMAQNPIEEDFTMPMQQIGGGAGDWYFYNSMTVN
                     NGKSEFRRRWGNRKLEDNWRRSNKSAALFTDNPASETSDTTARTDSVFVSTNPAIPSF
                     RQTSDKKSVDFYLAQIPFTEAQKEISNQQLATGLYNMAFVYKDKIEDYPLAYKSFGEF
                     QKRFGTDDRILETLYHRFLLASKENNPTLANQFRSEILAQYADSKYAEMLADPDFIRN
                     QQRMFEEQDFIYGKTYSAFTHSDYKTVFASTEEIKRKFPLSTLLPQFEFLNTLSIGKT
                     EGSEKFEKSLNELVENYPESNISAISKDILALLKQGNIAQQGQTFGSLLSKREQENLT
                     PEEQKAQSFSELKFAPHRMMLITDADNEAINKLQYNLAIFNFSRFMIKDFGFQLTQVD
                     AGRRALSVMNLASYNEGIWYQNTLATDTELAKQMDEMHVEQVIISEDNFGKLRTVFTL
                     DDYLAFEKEFLSKDVPTALLAAADAPKTEKKQTTVKIIDGTKTDLPPNPPETSSEQAL
                     KGELEMQQSDFVQNAEIAKEQPKEVSQNRNISIPTETVNQKPPKSPEGGLKERPKEVA
                     QSVNGQKPAEQSKSTEQPKLVQQQQVEKSKQTEPKQDDTQKIISEKYAQIPLSGGEGA
                     LYAFQANAPHYVAFYIPRGGRFDFEQVKKALDEYNAANYATMNLKVTLEEFGRESIIF
                     VSIFADANVAKSYFLRMLKEPTIVKATSGMNKRNLITTRDNLNKMLQNNALDVYFEFM
                     REYYLK"
     gene            7452..7838
                     /locus_tag="PLN75_05335"
     CDS             7452..7838
                     /locus_tag="PLN75_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRG03573.1"
                     /translation="MISLILLLVFAGLVVFTATYFNRRGKDEQNDIVINENSECCGAH
                     EVCERDNLQIIDTKIEYFDDEELDTLAGISPDNFTKKQIESVSEVFYSMHENDVAPWL
                     RSLQLRNIQLPDFLREEALMIVGERR"
     gene            complement(7902..8777)
                     /locus_tag="PLN75_05340"
     CDS             complement(7902..8777)
                     /locus_tag="PLN75_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRG03574.1"
                     /translation="MLKNLFKNSGVFGKLLMLFLCMFFLIFTGMALISLIPGNPADIH
                     ILKINQLILSVFTLLLPPIICSYFWYDKPLKSYSLHRLPELKQVILAIILIFSISPFI
                     NLLAYINEQMVLPEFLSGLENQIREMEDNAAELTRQMLDVNSTSALLLNLLVMAVIPA
                     LGEELFCRGALQNIFSEKFNKYTAVWIVAVIFSLIHFQMYGFLPRMVLGAVLGYLLVW
                     SGSLWLPILVHFINNATIVLASYFGRESEMLDAMENIGKAETWGYGIASAIISGVIIW
                     HIAHPIPPKGRDFAK"
     gene            complement(8920..9165)
                     /locus_tag="PLN75_05345"
     CDS             complement(8920..9165)
                     /locus_tag="PLN75_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRG03575.1"
                     /translation="MNIYFGNLSYKVRENDLQGVVAEYGEVTSCKVIKDRETGKSKGF
                     AFVEMTDDAAAKKAIEELNGAEFDGRAMVVKEAKPRV"
     gene            complement(9426..>9751)
                     /locus_tag="PLN75_05350"
     CDS             complement(9426..>9751)
                     /locus_tag="PLN75_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRG03576.1"
                     /translation="FIAIEYVLASFTLMITIVGIPFGLQTLKLAELALFPFGKKIVQN
                     DTSSGCISALMNIIWFFVGSLPIALTHLGLGLLFYITIIGIPFGNQHFKLMKLAFVPF
                     GKKIV"
     assembly_gap    9752..9851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9852..11110)
                     /locus_tag="PLN75_05355"
     CDS             complement(<9852..11110)
                     /locus_tag="PLN75_05355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRG03577.1"
                     /translation="MSMNVNNTNDWKQKAVTSSRSLNLVSDERKNLFLLSLADEIDKN
                     CAQILKANAYDLAGTEPENPLYDRLKLTPERIFAIAEDVRNVASLPSPTGRILMQSTR
                     PNGMKITKISVPFGVIGVIYEARPNVSFDVFSLCFKSGNACVLKGGSDAYHSNFAIVD
                     LIKKTLINSELDENIVTLLSAGREAASELMNATGYVDVIIPRGGKGLIDFVRQNSLVP
                     VIETGAGVCHTYFDEYGDLEKGINIVFNAKTRRVSVCNALDCLIIDEKRLADLPQICR
                     KMAEKNVIIYADEHAYEVLAGNYAENLLKTADAESFGTEFLDYKMAVKTVKNIGEAIE
                     HITKFSSGHSECIISEDASNIQIFSSQVDAACVYTNVSTAFTDGAQFGLGAEIGISTQ
                     KLHARGPMGLEELCSYKWLIEGDGQVR"
BASE COUNT         2892 a         2407 c         2297 g         3506 t
ORIGIN      
        1 ggtgtttcag tattattgga actattaaat gtattcactt tatttcttaa atattatctt
       61 ttatcaaaaa aatcaaacaa atcacagttc agacaaagtc ccccttctgg gtcccgatag
      121 ttatcgggat agggggctaa aactgcacca gtgaatacac atccatcgct tcatccaggc
      181 gggtaatgcc ctccagttcg agcagttcga tcaggaaact gacgtaaatc ggattgtcgc
      241 cgaacttgcg cagcaactgc aacgaggcat tggcgcttcc gccggtggca agcaggtcgt
      301 cgtgaagcag tattacatca tcgggttcga tggcatcctt gtgtatttca aggccgtcaa
      361 ctccgtattc cagctcgtac ttcaaactaa aagtttcggc aggaagtttt ccgggttttc
      421 gaagcagtac aaatccagcg ttgagtttat aagccagaat acttccgatt acaaagccac
      481 ggctttccac acccaccact ttggtaattc ccttgttttt gtagtactcg tagagtgtat
      541 cggtcatcag cttcaatatt tcaggatttt tcagagctgt tgtaatgtct ttgaactgaa
      601 ttccgggtat tggaaaatca ggaatgttgc ggatggaagc tttgattatg tcgcttgcat
      661 cacctgcccc ccaaccccca gaagggggag ttgttgagcg taattcacga ttatctgtat
      721 tttcgagatt tatatttgtc atttttaaaa ttattatttg tttttcctaa tccgtttatt
      781 tagaatgaat acttatattt atttagaatg aatacctgca ttatttttca agaacttgaa
      841 taataaacaa ttattccccc ttcgggaggg ttagggggca gtgctttcca gcattttttt
      901 tatcacatac tgcgccgaaa tttcccgatt ggccgcttcg ggaataacga tggattgggg
      961 actttcaatc acatcatctg ttacaatcat attgcggcga acgggcaggc agtgcataaa
     1021 ataagcattg ttggtaactg ccatttgccg ttctgaaacg gtccaggagc ggtctgtaga
     1081 gagaatttta ccgtaattcg gatcctgata agctgcccag tttttagcgt aaacaaaatc
     1141 tgctccttca aaagctttca tctggtcgta ttccacgcgc gcgtcacgca cgaatttttc
     1201 gctcagttca taaccttccg ggtgtgtaat cacgaaatca acatccgctt cgttcataaa
     1261 atcggcaaag gaattgggca cagcctgtgg caatgctttc gggtgcggag cccacgtgag
     1321 aaccactttc gggcgagcag tgcgtttata ttcctctatc gttatcaaat cggcaaatgc
     1381 ctgcaacgga tgccctgtag cggcttccat actgaaaacg ggtttacccg agtaatgaat
     1441 aaactgattg agaatggttt cctgataatc aaattcctta tcctcaaact gagcgaaagc
     1501 cctcacgcca atgatgtcgc aatacgttcc catcacggga atggcttcca gaatatgctc
     1561 cggtttgtcg ccgtccataa tcacgccccg ctcggtttcg agtttccagg caccctgatt
     1621 gatatcgagc accatggtat tcattccgag gttcattcct gctttttggg tactgagccg
     1681 ggttcgcagg cttgaattga aaaacaccat caacagcgtt ttattttcgc ccaactgctt
     1741 gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcggtt caaaaccgca
     1861 atctgctatc atattattca aaaaaatcac tctaaaatca cagttcagac ctttacttcg
     1921 gctccatggt taccaccgga attatcatct cttcgagcga aattccgccg tgctgaaacg
     1981 tgtttttgta gtagctcacg tagtagttgt agttgttcgg ataagcaaaa aagtcgtcgt
     2041 ttccggcaaa gatataggcg gaacttacat tggggcttgg caggccggct ttgtgcggct
     2101 gcaggatttc aaacacctct tttttgttgt aactcaggtt tttacccact ttgtagcgca
     2161 ggttggtgtt tacgtttttg tcgcccacca cttttatggc gttatttacc tgaatggtgc
     2221 cgtggtcggt tgttacgata accttataac cgcggtgagc aatggctttg aacaaatcat
     2281 acgtggccga atggcggaac cacgacagcg tgagcgagcg gtaggctttc tcgtcgttgg
     2341 ccagttcgcg catcattttg ctttcggtgc gggcgtgcga gagcatgtca ataaaattga
     2401 ggaccaccac gttcaggttg ttaccgtcca ttcttggaag ctggtcaatc actttctggc
     2461 aatagctgct ttcaaaaact ttgttgaatg agaaggagta tttcttcctg aaacggtcaa
     2521 actgggtctt caatagcgct tcttcgtgga tatttttccc ttcttcgtcc tcttcttcca
     2581 cccagaggtc cggaaacatc tgctgaatct gcaaaggcat caaacctgaa aaaattgcat
     2641 ttcgggcgta ctgtgtagcg gtcggcagga tactgcaata ggtttcttct tcggtaaaag
     2701 tgtaaaattc gttgagcact tcgcggatta cgagccactg gtcgtacctg aaattatcca
     2761 ccactaccag aaacactttt tcaccttcgt cgagcagcgg aaatacctta tttttcatca
     2821 gttccgggct aatcatcgga cgtccgttgg ggttgaccat ccagtccatg taattctttt
     2881 tgataaattt attgaacgtg ctgtttgcct catctttctg catccggagc atttcgtcca
     2941 tattattatc cgcttcggca agttccaatt cccagaaaac cagcttttta tacacttcca
     3001 cccagttttc ccaggtcaga ctgtcggata tctgcatccc gatgtttcgg aattcctgct
     3061 gataggtgct tgtggcgtgt tccgacacaa tttcgcgttt gtggatattt tttttcagtg
     3121 tgagcaggat ttgactcggg ttgaccggct tggtcaggta atcggctatc ttattcccga
     3181 tggccatgtt catgatgtcc tcttcctcgc ttttcgtaat catcaccagc ggtacattcg
     3241 gatcgatggt tttgatttgt gtgagcgttt ccagtccgct caaacccggc atgttttcat
     3301 ccagaaaaat gatgtcaaaa ctctccttct gacacaggtc aatggcatct ttcccgtttg
     3361 taacggtagt aacgctatat cctttttctt tcagaaaaat tacatacggt cgcagcaggt
     3421 caatttcgtc gtcggcccag agtattcggt tcatatattt tttatttacg atttacgatt
     3481 ttagatttac gattgggtta catttcgcgc ttttgtgttg acttttctgt aaagagtttt
     3541 ttagtattct ttctatttat atcgatttac atttgttttt ttgtcgattt tttaagcaga
     3601 tgattttcaa taattaatat cttctacacc caatcgtaaa tcgtaaataa ataaatcgta
     3661 aattatttca ggtaatattc cctcataaac tcaaaatata catccagcgc attattttga
     3721 agcatcttgt tcaggttatc gcgtgtggtt atcagattgc gtttattcat tcccgatgtt
     3781 gcttttacga ttgtcggttc cttgagcatc cgcaggaagt atgatttagc cacgttggca
     3841 tcggcaaata tgcttacaaa gataatactt tcccgcccaa attcttccag tgttaccttc
     3901 aaattcatcg ttgcgtagtt tgcagcgttg tactcatcca gcgccttttt cacctgctca
     3961 aaatcaaacc gtccgccacg cgggatgtag aaagccacat aatgcggtgc attggcttga
     4021 aatgcatata aagccccctc accccccgaa agggggattt gagcgtactt ttcggatatt
     4081 attttctgag tgtcatcctg tttcggttca gtttgtttag atttttcaac ttgctgttgc
     4141 tgaacaagtt ttggctgttc agtcgatttc gattgctccg caggtttttg cccattaaca
     4201 gattgagcaa cttcttttgg acgctcttta agtccccctt cgggagattt tgggggcttt
     4261 tgattgacgg tttcagtcgg aatagaaata ttccggtttt gtgaaacctc cttcggttgt
     4321 tctttagcaa tttcagcatt ttgcacaaaa tctgattgtt gcatttccaa ttctccctta
     4381 agagcttgtt ccgaacttgt ttcgggaggg ttagggggca gatctgtttt tgtcccgtca
     4441 ataattttta ccgtcgtttg ttttttctcc gtttttggtg catcagcagc tgccagcaat
     4501 gctgtcggga catctttgct taaaaattcc ttttcgaaag ccaggtaatc gtccaacgtg
     4561 aaaacggtac gtaattttcc gaaattatct tccgaaatta tcacctgctc cacgtgcatt
     4621 tcgtccatct gtttagccag ttctgtatct gtggcaaggg tattttggta ccagatacct
     4681 tcgttgtacg atgccagatt catcaccgaa agcgcacgcc ttcctgcatc gacctgagtc
     4741 agctgaaatc cgaaatcctt aatcataaac cgggagaaat tgaaaatggc caggttgtat
     4801 tgcaatttat taatcgcttc attgtcagca tcggttatga gcatcatccg gtgaggcgca
     4861 aatttcagtt cagaaaaact ctgtgctttc tgttcttccg gggtgaggtt ttcttgttcg
     4921 cgtttggaga gcagcgagcc gaatgtttgt ccctgttggg caatatttcc ctgtttcagc
     4981 agagccagaa tgtctttaga aatggcgctg atgttgcttt cgggataatt ctccaccagt
     5041 tcgttcagcg acttttcaaa tttttctgaa ccttccgttt ttccgatact gagcgtgttc
     5101 aaaaactcaa attggggtag caaagtggag agcggaaatt tccgttttat ctcttctgtg
     5161 ctggcaaaaa cggtcttata atcgctgtgc gtgaatgccg agtaggtttt tccgtaaata
     5221 aaatcctgct cttcaaacat tcgttgctga ttgcggataa agtccgggtc agcaagcatt
     5281 tcggcatatt tgctgtcggc gtattgcgct aaaatctcgc tgcgaaactg attggcgagt
     5341 gtagggttat tctctttgga tgccagcaga aagcggtgat acagcgtttc cagaatgcgg
     5401 tcgtccgtac caaatcgctt ttgaaattct ccaaacgact tgtaagcgag cggataatct
     5461 tcaattttat ccttgtaaac aaatgccata ttgtacagtc cggtggccag ttgctgattt
     5521 gaaatttctt tttgagcttc ggtaaacggt atctgagcca ggtaaaaatc cacgcttttt
     5581 ttgtcggagg tttgcctgaa cgacggaatg gccggattgg ttgaaacaaa tacagaatcg
     5641 gtgcgagcag ttgtgtctga tgtttctgaa gccggattat cggtaaacaa tgcagccgac
     5701 ttgtttgacc gccgccagtt atcttccagt ttgcggtttc cccagcggcg gcggaattcc
     5761 gattttccgt tgttgacagt cattgaattg tagaaatacc aatcgcccgc gccaccgcct
     5821 atctgctgca ttggcatggt gaaatcttcc tctatcggat tctgagccat cagtttgcct
     5881 tctgcttccg ctttttcatc ttcttttatc ttttcaatca ccctgttgat agccagcaga
     5941 cgctcttttt ccggcatcac ggacaatgcc tgcaaactgt cctgtaaatg cactgtttcg
     6001 tactctttca ccagctcgcc gagtacttcg gcgcggtggc ttattttttc gtaatcatca
     6061 aattccttgg tgtaaatttt agcagcttcg tcgtagtacg gatgtgcttt tacataatca
     6121 cgctgctgat agtacagatt gcctaatata accagcgcaa ctcctttctc aactccgttt
     6181 cgggtgcttt ttccgatggc caggttgtaa ttttcaatgg ctttagcgct gtcttttttc
     6241 tggatgtaaa tattgccgag tgctgtataa atctggtcga gataatcttt attatttcgg
     6301 tttcgggcca tttttttcag ttctctttcc atacctgcag ggttggcagt ttttacctgt
     6361 gccagattga tgcgggcatt aaagtccatt tcatatggag gtgtcgattt gatgacctgg
     6421 gtgtagtaat tttcggcttc ttttttattt ccggtttttt ccagcagctg tgccagcaca
     6481 aatttgaagc ggttcctgag ttttttattt ttttcctctt ttattgctgt tttcagaaaa
     6541 ggtattgcct ccggatactg atttttcttt atcagcagat cagcatttgt cgatgtatat
     6601 agtccgatgt tatcgccttt gagttgattt ggttttattt tttcgatcac atcctcagct
     6661 tcgtatatcc agtccatctc ggcataggca cgtgcaatcc agagctgagc tgccattacc
     6721 aggtcgggat ctgtagcata aaaccgtgta atgtacgaaa aagtgcctac cgaacccaga
     6781 aaatctcctt tgtgaaattc ggctttaccg agctgcatcc aggcattctt caattcagga
     6841 ttgaattcat tttgattata aaaagcctga taatcagggt cgttgtagcg cttgtaatcc
     6901 cgttcgggct ttttctttat agagtgcagt ttgatggctt tacggctttt ctcgatagtt
     6961 cggttcatgt tgccggcagc acccgaagtg ttggaatgat tgctaaccgg aaacatgttg
     7021 attatctgag agtaatcgtc cttattggct ttcgcaatgt tttccatccc ttcgttgtag
     7081 ctcgtattcc cgttgaaagc aatgttaaac ctggttagca tagcatggta acgacgggac
     7141 atgccggtgt ttttgctcgt ggagcaactg cttgccacga gcaaaaccga aataaaaaaa
     7201 atgatatgta aaaactttat tctcaatttc atcaaatttt ctttaataca gggtcagaat
     7261 ccaacttttt taaaaccgac aatctgctct tttattgtgc aaaatcaacg aatagagcgt
     7321 taaatattct aattacgggc tttatttctt aactttgcag actgaaaatt gttatctgtt
     7381 tttgaaacat aaagcagttt tttacattac aaagaacact gatttagaaa gtctgtaaat
     7441 tttttattag aatgatttct ttaattttat tattggtttt tgccggtttg gtagtattta
     7501 ctgctactta cttcaaccgg agaggaaaag atgagcaaaa tgatattgtt atcaatgaaa
     7561 attccgaatg ttgcggtgcg catgaagttt gcgaaaggga taatttgcaa ataattgaca
     7621 caaaaataga atatttcgat gacgaagagc tggatacgct ggccggaata tcgccggata
     7681 atttcacgaa aaaacagatt gaatctgttt cagaagtgtt ttattcgatg catgaaaacg
     7741 atgtggcgcc ctggcttcgc agtttacaat tgcgtaatat ccaactgcct gattttctgc
     7801 gcgaagaagc gctgatgata gtgggggaga ggagataaag cccctccaac ctccccaaag
     7861 gggaggctcc ttttcagaat gctaaaaatc caatcaaatt tttattttgc aaaatccctc
     7921 cctttgggag ggattgggtg ggctatatgc caaataatca ctcccgaaat tatagcactt
     7981 gctattccat atccccaggt ttcggctttt ccgatatttt ccatggcatc aagcatttcg
     8041 ctttcgcgcc cgaaataaga cgccagcact atggtggcat tatttatgaa atgcaccaaa
     8101 atcggcaacc acagactccc cgaccaaacc agcagatatc ccagcactgc gccgagcacc
     8161 attcgcggta gaaatccata catctgaaaa tgaatgaggc taaaaatgac agccacaatc
     8221 caaactgctg tatatttatt aaatttttcc gaaaaaatat tctgtaaagc gccccggcaa
     8281 aacaattctt cgcccaaagc aggtatcact gccatgacca gcagattaag aagcagtgca
     8341 ctcgttgaat ttacatcaag catttgacgc gtgagttctg ctgcgttgtc ttccatttcc
     8401 cgaatctggt tttccagtcc ggaaagaaac tccggaagca ccatctgctc gttgatatag
     8461 gcaagcaggt tgataaacgg cgaaatgctg aaaataagaa ttattgccag tattacctgt
     8521 ttcaactccg gcagacggtg taatgaatag gatttcagcg gtttatcgta ccagaaataa
     8581 ctgcagataa tcggtggaag caaaagtgta aaaacggaaa gtatcagttg attgattttc
     8641 agaatatgaa tatcagcagg attaccgggg attaaactta tcagagccat tcctgtgaaa
     8701 atcaggaaaa acatacataa aaagagcata agcaatttgc cgaatactcc tgagtttttg
     8761 aataaatttt taagcatagc aactaaatgt gtgtacaaaa gtacaaaaaa cctgaggtat
     8821 tttaatttat ctgactgaag tgcgcgtttt acaaaaaaaa atcttccatc tccggcgagg
     8881 agtcagaaga ttttcaatac cctttacctg aaaaaaaaat taaacgcggg gtttagcttc
     8941 tttaaccacc attgcgcgac cgtcaaattc tgcgccgtta agttcttcga ttgctttttt
     9001 tgcagcagca tcatcggtca tttccacaaa agcaaaccct tttgatttgc ctgtttcgcg
     9061 atctttaatc actttgcatg aagttacttc accgtactca gccactacac cctgaaggtc
     9121 attttcccga actttatagc tcaggtttcc aaaataaatg ttcataatta caaaaataaa
     9181 aaagattgaa tataacagat ttgataaaac agaaagagta ttgaatgcgg aaggcttaaa
     9241 aaacaatttt cattcaaata atgaaattcc cgaagagaat tgactgattt agaacctgac
     9301 gaaaaataca ccaactattt tcacagcaaa gaaaaacatt ttttttgaat caacaaacaa
     9361 aaaaataaat atttgaactt taattcgttg tcagttaaat tattggctat atgagatggc
     9421 accatttaca ctattttttt cccgaaaggc acaaaagcca gtttcataag cttaaaatgc
     9481 tgattaccaa atggaatgcc aataattgtg atgtaaaaaa gcagtccgag tcccaggtgt
     9541 gtaagtgcaa ttggcaggct gcccacaaaa aaccagataa tgttcatcaa tgccgaaata
     9601 caccccgaag aagtatcgtt ttgaacaatt tttttcccga aaggaaacag ggccagttcg
     9661 gcgagtttca aagtttgcag cccgaacgga ataccaacga ttgttatcat cagtgtaaag
     9721 cttgccaata catattcgat ggcgataaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn ntatctcacc tgtccatccc cttcaatcag ccacttgtaa gagcaaagtt
     9901 cctccagtcc catcggaccc cgggcgtgta gcttttgtgt gctgatgccg atttcagcgc
     9961 ccaaaccaaa ctgagcccca tcggtaaatg ccgtagaaac attggtatat acacaggcag
    10021 catcaacctg tgaactgaaa atctgaatgt ttgaagcatc ttctgaaatg atacattcgc
    10081 tatgaccgga actgaatttt gtgatatgct caatagcttc cccgatattt ttcaccgttt
    10141 tcaccgccat tttatagtcg agaaattcag ttccgaaact ttcagcatcg gccgttttca
    10201 acagattttc ggcgtaattg ccggccaaaa cctcataagc gtgttcgtcg gcataaatga
    10261 taacattttt ctctgccatt ttcctgcaaa tctgcggaag gtctgccagt cgtttctcat
    10321 cgattatcag acagtcgagg gcattacaca cgcttacacg gcgtgttttt gcattgaaaa
    10381 cgatgtttat tcccttttcc agatcaccgt attcatcaaa ataagtgtga cacacgccgg
    10441 ctccggtttc aattaccgga accagtgaat tctgtcttac aaagtcaatt aaacccttcc
    10501 cgccgcgtgg aataatgaca tctacgtagc ctgtggcatt catcagctcg gaagcagctt
    10561 cacgtcctgc agaaagcaat gttacgatat tttcgtccaa ttcgctgttt atcagtgttt
    10621 tttttatcag gtctacgatg gcaaaattgg aatgataagc atcgctaccg cctttgagca
    10681 cacaggcatt gccggacttg aagcaaagcg agaacacatc gaaacttacg ttgggacggg
    10741 cttcatagat tacacctatc actccgaaag gaaccgagat tttagtgatt ttcattccgt
    10801 ttggccgtgt ggattgcatc agtattctgc ccgtaggcga ggggagtgat gccacgttac
    10861 gtacatcttc agcaattgca aaaatgcgtt caggcgtaag tttcaacctg tcgtaaagtg
    10921 gattttcagg ctccgttcct gccaggtcat aggcattggc ttttagaatt tgagcgcaat
    10981 ttttatcgat ttcatcggct aaagatagca ggaaaaggtt tttccgttcg tctgaaacca
    11041 aattcaggga gcgggaagaa gtcaccgcct tttgtttcca gtcattggta ttgttgacat
    11101 tcatactcat tctttttttt tgatgaaaac tgtaatcagt tttttaaact cagtgtatat
    11161 tgaacacgtt gtttttttac aggtaataat tcttgcagga atacatccac aggcgatatg
    11221 ctataataaa ttcagcacac ggtccaactc tttcgacagt atatttttga aatagtctat
    11281 ttgctctatt tctttcatta tt
//
LOCUS       DAORKP010000070        10892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_688_length_10892_cov_9.171173, whole genome shotgun sequence.
ACCESSION   DAORKP010000070 DAORKP010000000
VERSION     DAORKP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_688_length_10892_cov_9.171173"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1101)
                     /locus_tag="PLN75_05360"
     CDS             complement(193..1101)
                     /locus_tag="PLN75_05360"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03578.1"
                     /translation="MEKLIRHIETLLRRHDYVIVPELGGFVLQRQSSVITDEGIEPPL
                     TVVSYNPLMKTSDGLLAIELSRAEHISFREAVQLITAEVETAKVLLEKNKKLELGKLG
                     FLSIDSGDKVTFRPSANAGFIPSNFGLETLHVAAIQKADEEEKRVIRIVIPSRRKLTR
                     YAAAAAVIAGLVFGAPKMSEAYRDFANLYPASLFDKTEIQPENQTAQPVQTPAEEPET
                     AVAVAEPSHHVIVSCMATLKDAEEYQEWLKSLNYQHTRILPPVKTYRIAIESFTTKEE
                     AIVYMQELRNSKPQFADAWVLSDSGK"
     gene            complement(1221..2318)
                     /locus_tag="PLN75_05365"
     CDS             complement(1221..2318)
                     /locus_tag="PLN75_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRG03579.1"
                     /translation="MNLQKIGLKRLDTYIIWKFLGTFFFSIVVILSIAVVFDVTEKID
                     DFYEHNANLHAIVFDYYLSFLPYYAYLFTPLFTFISVIFFTSKMANDTEIVAILASGV
                     SFNRLMRPYFIASMIITVFAFLLGAFVIPSSTEKLIDFEKKYIEPEKASNNARNVQME
                     VEKGVVLYIERFEITDNTGYRFSLEKFEDKTLVSRITAETVKWDSLYNWKLFNYVQRD
                     FNGMREKLVRGASKDITIKVLPKELFITATEAPQLSLTELRNHLRKQKERGVGNIQAF
                     EDEYYRRFSTAISVLVMTLIGVSLSSRKVRGGMGLNLGIGLGLSGLYVIFTTMSTTFA
                     VNGVMPTLLAVWIPNITFFIVAVYLFIRAPK"
     gene            complement(2393..3523)
                     /gene="tgt"
                     /locus_tag="PLN75_05370"
     CDS             complement(2393..3523)
                     /gene="tgt"
                     /locus_tag="PLN75_05370"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRG03580.1"
                     /translation="MNFTLQHTDPNSRARTGLIKTDHGEIKTPIFMPVGTVGSVKGVH
                     FQELKDDIGAQIILGNTYHLYLRPGIKILEQAGGLHRFNSWDKPILTDSGGFQVFSLS
                     NTRKMHEEGVTFSSHIDGSKHLFTPEGVVDIQRSIGADIVMALDECTPGTADYRYARE
                     SMELTHRWLARGVKRFVETEPKYGYSQTFFPIVQGCVYKDLRQQSASFAASLDREGYA
                     IGGLAVGEPTEVMYEMIEVVNEILPAEKPRYLMGVGTPVNILEAIERGVDMFDCVMPT
                     RNGRNGMVFTSRGIMNMKNEKWKNDFSPLDESGTAAVDHIHSKAYLRHLFVSKELLAM
                     QIASIHNLAFYLWLVGEARSHIEAGDFTSWKKEMVVQFAHRL"
     gene            complement(3712..4305)
                     /gene="hisH"
                     /locus_tag="PLN75_05375"
     CDS             complement(3712..4305)
                     /gene="hisH"
                     /locus_tag="PLN75_05375"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRG03581.1"
                     /translation="MNVVIIKYNAGNIRSVDFALQRLGVRAVVTDDFDEICAADRVIF
                     PGVGEASSAMEYLNERKLDTLITGLKQPVLGICLGMQLMCKESEENNTKCLGIFDLTV
                     RRFPETGFKIPQIGWNNIYDLSSPLFSGISENEYMYFVHGYYVEKSTQTIARADYALE
                     YSAAISKDNFYAVQFHPEKSGVMGQKIIDNFLKIPAP"
     gene            complement(4479..7292)
                     /locus_tag="PLN75_05380"
     CDS             complement(4479..7292)
                     /locus_tag="PLN75_05380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRG03582.1"
                     /translation="MLKMKTLVAALLMVFAVNLSAQQMQPLPLDPKIRYGKLENGLTY
                     YIRHNEEPKQRAEFHIAQNVGAILEKDEQNGLAHFLEHMAFNGTKNFPDKGIINYFEK
                     QGVKFGYDINAYTSLDETVYRLSNVPTARQGLLDSALLVLHDWSGFISLKEEEIDNER
                     GVIREEWRTGNNASRRMFRKSNELRYPGSQYSKRDVIGDTAVINNFSYDALRAYYKKW
                     YRPDQQAIVIVGDVDVDLMENKIKEMFADIPKKENFGERPVYEIENNKEPIVALVTDP
                     EASQTQIRIDFKKDKLPAEVQLSMVGYVMNTVNNIVSSVMNERFTEISQQADAPFVGA
                     FSYYGELVKSKDAFINIVIPKEGKEIEGFRALALEMEKLKRFGFTNAEVERAKTNLLK
                     DMEKSYNERDNQNSQRLAREYIRHYLSNEPVPGIESEYETVKMILPQITTAAVNQVAK
                     SYVVDENVIISMNAPEKPEVKVPEKTQLLAVLSEAKQAELTAKAEEEATRPLLDKTPK
                     AGKVKKITKNQDVEVTEMTLSNGIKVVFKPTTFKKDEISLRAFSDGGLSKVKNIDDLY
                     SASLATSIVSANGIGNFTATDLSKALTGKIASVSPYINQYSEGMNGNSSVKDLETLLQ
                     LVYLHFTAPRKDDNSYGALLNMLKTSLANAEKNPNKAFSDSVRKTTSGNDPRVLIQNM
                     QMIDKINQDKAIEIYKQRFANPADFTFVFVGNIDPNDKATQKLLATYLGGLKTAKAKE
                     TYDKVYRFTPKGKIKNYFNREMQTKKASNRIVYTADLPFNIHTNMNVSAIGDILDIRY
                     LESIREKEGGSYGVGVYGGVGNTPKDEATILMQFDTDPEKQARLMEIIHQEVTDIVNN
                     GPKAEDVQKVKENLLKQYAQDLEQNSWWAATLKSYYEDGINNLKDYKSAVEAMNGQTI
                     QKTLKQIVDQGNVIEVVMLPEKK"
     gene            7606..8097
                     /locus_tag="PLN75_05385"
     CDS             7606..8097
                     /locus_tag="PLN75_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRG03583.1"
                     /translation="MSESIVISKNQPKEVVYSELFPQLKSLLEGETDLIANLANVAAA
                     LKEVFGWWWVGFYLVKGNELVLGPFQGPVACTRIAFGRGVCGMAWKEKRSVLVPDVNQ
                     FPGHIACSSASVSEIVVPVFNENNEVFAVLDVDSERYDVLDEVDVEWLEKIVLMKNLK
                     FTI"
     gene            complement(8196..8726)
                     /locus_tag="PLN75_05390"
     CDS             complement(8196..8726)
                     /locus_tag="PLN75_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03584.1"
                     /translation="MTKPSNVKALETGTGQSWEYWLEFLESIKARELPHDEIAVRVNT
                     HGAGAWWSQGITVAYEQHIGRRIPGQTCDGDFQVTVSKTIGGNMDDALEKWLKRVEGM
                     TEFNRVKITREPTISQTEKWRYWRCGLEDKSNVSVNIQTKAGGEKSLLAINHDKIQEA
                     GDLEKWRAFWKSFTAP"
     gene            complement(8858..10201)
                     /gene="sufD"
                     /locus_tag="PLN75_05395"
     CDS             complement(8858..10201)
                     /gene="sufD"
                     /locus_tag="PLN75_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRG03585.1"
                     /translation="MVLEQQYIELYEQHSETIRKHSASALNIGRDKAFEKFRELGFPT
                     PRVENYKYTDLREPLTVDYGLNIKRMNIPVNPYEVFKCDVPGISAYLYFVVNDAFYPV
                     NYEKNKSLPEGVVITSLRKASEEYPELVEKHISKLAEKHVDGMIAFNGAFAQDGFFIY
                     VPKNVLLDHPIQIINIMRSDVDFMANSHNLVIVEQGAKAQVLVCDHTVDDVRFFSNRL
                     TEVFVGENAFYEHYKLENVSRKTTDVSTLLIDQMQNSNVLGNLLTLHNGMTRNTVEID
                     LDGENCETYLCGMALSDGKQVIDNYTSILHNKPNSHSNELFKYILDEEAKGGFTGRLY
                     VAKDAQKTLAYQNNKNILLNRTAKMRTKPQLEIYADDVKCSHGATIGQLDEKAMFYLR
                     QRGISEKEARMLLMYAFTADVIENIRIDALKDRIKMLVEKRLRGELSKCEGCSIC"
     gene            complement(10414..>10892)
                     /gene="sufC"
                     /locus_tag="PLN75_05400"
     CDS             complement(10414..>10892)
                     /gene="sufC"
                     /locus_tag="PLN75_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRG03586.1"
                     /translation="VEIPGVSMVNFMRAAINEQRKYRGQEPVSATEFLRLMREKKELV
                     EMDSQLTNRSVNEGFSGGEKKRNEIFQMAMLEPKLAILDETDSGLDIDALRIVANGVN
                     KLKTPENASIVITHYQRLLDYIIPDFVHVLHNGKIIKSGDKSLAMELEEKGYDWLK"
BASE COUNT         2787 a         2364 c         2286 g         3455 t
ORIGIN      
        1 ctttttctcg aaaagcggac gcaaagatag tgtatttttt tgaaatgaaa aagagatagg
       61 gttttatttt aagaaacaag tagttattat tcattcgttt attcgttttg ggtagatttg
      121 atgacaggtg aaatgtattt cgaataaacg cccgaacagt tgaacttccg aacagttgaa
      181 cttgtgaaaa aactatttcc cggagtcgct cagcacccat gcatcggcaa actgtggttt
      241 gctattacgc agttcctgca tataaacgat ggcttcttct tttgtggtga aagattcgat
      301 ggctattctg taggtcttta caggtggaag tatccgtgta tgttgataat tcagtgattt
      361 gagccactcc tgatactctt cggcatcttt gagtgtcgcc atgcaggata caattacgtg
      421 gtggctgggt tcagcaactg caacggcagt ttccggttct tcggcaggtg tttgtaccgg
      481 ttgagccgtc tgattttcag gttgaatttc agttttgtcg aaaagtgaag ccggatacag
      541 attggcaaaa tcgcgataag cttcgctcat tttgggtgct ccgaaaacca atccggcaat
      601 cacagcggca gcagcggcat atcgtgttaa ctttcttcgt gatggaatca caatccgtat
      661 gacacgtttt tcttcttcgt cggctttctg aatggctgca acgtgaagcg tttccaatcc
      721 gaagtttgaa gggatgaagc cggcgttagc tgaaggtctg aatgtgacct tatcgccgga
      781 atcaatggaa agaaatccca gtttcccaag ttccagtttt ttgtttttct ccaaaagtac
      841 ttttgccgtc tccacttcgg ctgtaataag ctgcacagct tcgcgaaaac tgatatgctc
      901 tgctctggaa agttcgatag caagcaatcc gtcggatgtt ttcatcagcg gattatagct
      961 tacaaccgtc agcggtggtt cgataccttc gtcggtgata accgatgatt gtctttggag
     1021 tacaaatcca ccgagttcgg gcacgattac gtaatcgtga cggcgaagca atgtttcgat
     1081 atgacgaatt aatttttcca tcgttgcaaa tatagacatt cctgctgaat ggaaaacaac
     1141 tttggcttaa aaatttatca acaacttatg aaatgagact acttgttaaa ataaaccgga
     1201 gtagaataac ttgtaatcaa ctacttaggt gccctgataa aaagatatac ggcaacgata
     1261 aagaaagtga tattcggaat ccatacagcc agcagcgtag gcattactcc attcacggcg
     1321 aaagtagtgg acatggtggt aaaaatcaca tacaatccgc tcagccccag gccaatgccc
     1381 aaattcaacc ccatgcctcc ccgcactttc cgtgaagaga gcgagacacc gatcagcgtc
     1441 atcacaagta ccgaaatggc tgtagagaat ctgcggtagt attcatcctc gaatgcctgg
     1501 atgtttccca caccccgttc tttttgtttt ctcaggtggt tgcggagttc ggtaaggctg
     1561 agttgcggag cttcggtggc agtaataaat agttccttcg gcagcacctt gatggtgata
     1621 tctttgcttg caccccgtac cagtttctcg cgcatgccgt tgaaatcgcg ctgcacgtag
     1681 ttgaaaagtt tccagttata gagcgaatcc cacttcactg tttcggcagt aatacgtgat
     1741 acaagggttt tatcctcaaa tttttcaagc gaaaagcgat aacctgtatt atcagtgatt
     1801 tcaaaacgtt cgatatacaa taccactccc ttttcaactt ccatttgaac attccgggcg
     1861 ttgtttgatg ctttttcggg ttcaatgtat tttttttcga aatcaatcag tttttcggtt
     1921 gacgatggga tgacaaaagc tccgagcagg aaagcaaaaa cagtaataat catcgaagcg
     1981 ataaaataag gccgcattaa ccggttgaaa cttactccac tggccagaat agctacaatt
     2041 tcggtatcgt tggccatttt tgaagtaaag aatattaccg aaataaaagt gaaaagcgga
     2101 gtaaacaggt acgcgtaata gggcagaaaa ctcaggtaat aatcaaatac aatggcatga
     2161 agattggcgt tgtgttcgta aaaatcatca attttttcgg taacgtcaaa aaccactgca
     2221 atacttaata tcacaacgat ggaaaagaaa aatgtaccaa gaaatttcca gatgatataa
     2281 gtatccagtc gcttcagtcc tattttttgt aagttcattg ttcgttgatt gattgatcgg
     2341 ttgatttttt aaggagtaaa atctgcaagc gggttacaga acaatctttt ttttaaagcc
     2401 tgtgtgcaaa ctgtaccacc atttcctttt tccagctcgt aaagtctccg gcttcgatgt
     2461 ggctgcgcgc ttcgcccacg agccaaaggt agaacgccag gttgtgaatg gaagctatct
     2521 gcatagccag caattccttg ctcacaaaca ggtgtcgaag gtatgccttt gaatgaatat
     2581 gatccacggc agctgtgccg ctttcatcaa gcggcgagaa atcattcttc cacttttcgt
     2641 ttttcatatt catgattcca cgggaggtaa ataccattcc atttctgccg tttcgggtgg
     2701 gcattacgca gtcaaacata tctactcccc gttcgatagc ctccaggata ttcaccggag
     2761 tacccactcc catgaggtaa cggggtttct ctgccggcaa aatttcattc accacttcta
     2821 tcatttcata catcacttct gtaggttcgc ccacggcaag tcctccgata gcatatcctt
     2881 ccctgtccag cgaagccgca aacgatgccg attgttgtct taaatccttg taaacacagc
     2941 cctgtactat cggaaagaag gtttgtgagt atccgtactt cggttcggtt tcgacaaacc
     3001 gtttcacacc cctggcaagc cagcgatgtg taagttccat agattcacgg gcatacctgt
     3061 aatcagcggt acccggcgta cattcatcca gcgccatgac gatatcggct cctatgctgc
     3121 gctgtatatc taccacacct tcgggtgtga agaggtgctt gctgccatca atgtgcgaac
     3181 tgaaggtcac accttcttcg tgcatttttc tggtattgga aagcgaaaat acctgaaaac
     3241 caccgctatc ggtcagaatg ggtttatccc agctgttgaa cctgtgaagt cctccggctt
     3301 gttccagtat tttaataccg ggacgcaggt aaagatggta ggtatttcca agaataatct
     3361 gagctccgat atcgtctttc aactcctgaa aatgcacccc ttttacactc ccgacagtgc
     3421 ctacaggcat gaaaatgggt gttttgattt caccatgatc ggtcttaatc aggccggtac
     3481 gtgcacggct gttcgggtcg gtatgttgta aagtaaaatt cataaaacaa taaactgtcc
     3541 ttttaaacca ctcctattgt gggacttttt tttttgaata cgggtagttt tcagattttg
     3601 ttcaataata tttatttgtt tcaattgttt taaaaataca tgtcccgaaa atcaaactat
     3661 tctgtagtat tttactcaaa tgcagtattt taagagtccc tcaaggggga tttagggggc
     3721 cggtattttt aaaaaattat caattatttt ctgccccatc actcccgatt tctccgggtg
     3781 aaactgcacg gcgtaaaaat tatctttgga gatggccgca ctgtactcga gggcataatc
     3841 ggctctggct atggtttgcg tacttttctc cacgtagtat ccgtgaacga aatacatgta
     3901 ttcgttttcg gagattccac tgaaaagcgg cgatgagagg tcatagatgt tgttccaccc
     3961 gatttgggga attttgaatc ctgtttcggg aaaacgcctt actgtcagat caaaaattcc
     4021 caaacatttc gtgttgtttt cttccgattc cttgcacatg agctgcatgc cgaggcaaat
     4081 acccaataca ggctgtttca gaccggtgat aagggtgtcc agttttcttt cattcaaata
     4141 ctccatagcc gaagaagctt cgccaactcc cggaaaaatg accctgtcgg cagcacaaat
     4201 ctcatcaaaa tcatcggtaa caaccgcccg gactcccaat cgctgcaatg caaaatcaac
     4261 agaacgtata ttgcctgcgt tgtatttaat gattactacg ttcatatttt ctatttgaaa
     4321 ctgcaaaagt ataaaacaaa ttccttttaa caatcgattt taatgattgt tttcgtttaa
     4381 tgatagtagc atgtagccgc cggaaaaaaa agcgggaaga gtttcggcga attgctgaaa
     4441 ctcttcccgt ttctgaaaaa aagcggaaaa cgagctgctt attttttttc gggcagcatg
     4501 accacttcaa tcacattgcc ctgatcaact atctgtttga gggttttctg gattgtctgg
     4561 ccgttcatcg cttctactgc tgatttgtaa tctttgaggt tgttgattcc atcttcgtaa
     4621 tacgatttca gcgtggcagc ccaccaggag ttttgttcca ggtcctgagc atactgtttg
     4681 agcagattct ctttcacttt ctgcacatct tcagctttcg gaccgttgtt aactatatct
     4741 gttacttcct gatgaatgat ttccatcaag cgggcttgtt tctccggatc ggtatcgaat
     4801 tgcattagaa tcgtagcctc gtctttcggt gtatttccca caccaccgta aactcctact
     4861 ccataggagc ctccttcttt ttcacgaatg ctttcgaggt agcgtatgtc cagaatatca
     4921 ccaatagcag acacattcat atttgtatga atattaaacg gcaaatcggc agtgtaaacg
     4981 attcggtttg aagctttttt ggtctgcatc tcccgattga aatagttttt aatctttcct
     5041 ttgggagtga accgataaac cttatcgtag gtttcttttg cttttgctgt tttcaaacct
     5101 cccagatagg tggcaaggag tttttgggtt gccttgtcgt tcgggtcgat atttcctacg
     5161 aatacaaagg taaaatcggc cggattggca aaacgctgct tgtatatttc gatggctttg
     5221 tcctgattaa ttttatcaat catttgcata ttctggatga gtacacgcgg atcgttgccc
     5281 gaagtggttt tccttacgct gtcactgaat gccttattcg gatttttttc agcgtttgcc
     5341 agcgatgttt tgagcatatt cagcaaagct ccgtacgagt tatcgtcttt gcgtggtgca
     5401 gtgaagtgca ggtatacgag ttgcagaagt gtttcgaggt cttttaccga cgaatttccg
     5461 ttcattcctt cggaatactg gttgatgtaa ggacttactg atgctatctt tccggtaagc
     5521 gccttgctca ggtcagtagc tgtaaaattg ccgattccgt tggccgaaac gatgctggta
     5581 gctaaacttg cagagtataa gtcatcgata ttttttacct tggataaacc accatcactg
     5641 aatgcacgaa gcgaaatttc atctttcttg aaagtagtag gtttgaatac cactttaatg
     5701 ccattgctca gggtcatttc agtaacctca acatcctgat ttttggtaat ttttttcacc
     5761 ttacctgctt tcggagtttt gtcgagcagc ggacgtgtgg cttcctcttc ggctttggct
     5821 gttagttcgg cttgttttgc ttcagacaat acggcgagca gctgagtttt ttcgggcact
     5881 ttcacttccg gtttttcggg tgcgttcatc gatataataa cgttttcgtc cacaacatac
     5941 gatttggcta cctgatttac agctgcggtg gtgatctgcg gcagaatcat tttcacggtt
     6001 tcgtattccg attcaattcc cggaacgggc tcgttgctga ggtaatggcg gatgtattca
     6061 cgtgccagac gctgactgtt ttgattatcg cgttcgttgt acgatttttc catgtctttc
     6121 agcaagttgg tcttggcacg ctccacttcg gcattggtaa aaccgaaacg tttcagtttt
     6181 tccatctcca gtgcgagtgc cctgaatcct tcgatttctt tcccttcttt tggtattacg
     6241 atattgatga atgcgtcttt tgattttacg agttcgccgt aatatgaaaa agcacctaca
     6301 aaaggagcat ctgcctgctg gctaatttcg gtaaagcgtt cgttcatcac cgaactgact
     6361 atgttgttta cggtattcat tacataacct accatcgata gttgtacttc ggcaggtaat
     6421 ttatcttttt tgaaatcgat acgtatctga gtttgtgaag cttcgggatc ggtaacaagg
     6481 gctactatgg gttccttgtt attttcgatt tcgtaaacgg ggcgttcgcc aaagttttcc
     6541 ttttttggaa tgtcggcaaa catttccttg attttgtttt ccatcaggtc cacatccaca
     6601 tcgccaacaa taacgatagc ctgctgatcc ggacggtacc attttttgta ataagctctg
     6661 agtgcatcgt acgagaaatt attgataact gccgtatcgc caattacatc tcttttggag
     6721 tactggctgc ccggatagcg gagttcgttt gatttgcgga acattcttcg gcttgcattg
     6781 tttccggtac gccattcttc gcggattacg ccccgctcgt tgtcaatttc ctcttctttc
     6841 agcgagataa atcctgacca gtcatgcaat accagcagtg cgctgtccag caatccttga
     6901 cgggcagtag ggacattgga aaggcgatat accgtttcgt cgagcgaagt gtatgcgttg
     6961 atatcgtatc cgaattttac gccctgtttt tcgaaatagt tgataatgcc tttgtccgga
     7021 aagttttttg tcccgttgaa agccatgtgt tccagaaagt gagcgagtcc gttctgttcg
     7081 tccttttcaa ggattgcacc cacattctgt gcaatatgaa actctgcacg ttgttttggc
     7141 tcttcgttgt gacgaatgta gtaggtaagt ccgttttcta attttccata acggattttg
     7201 ggatccagcg gcagcggttg catctgctgt gctgacagat ttactgcaaa taccatcagt
     7261 aaagctgcaa ctaaagtctt cattttaagc atattgattt tatttaagtt aatttgagca
     7321 ttttccgatt cgtaaaaaaa tctgaaagat tacacaaagg aaattatttt ttaacaaaca
     7381 gccaattctg aaaagaattt aagcgttaaa taaataaaaa aacggtttga aagtgacttt
     7441 tgttaaatat taccttacaa attgataact tcatcggttg gtagtatttt tttaaaaaaa
     7501 tgtcaaaatc tattttactt accttttttc tcatcccaat ttcaagtttg tgaaacatgc
     7561 agatttgatt tacctttgca tgtatcaatc tgaactaaaa attgtatgtc tgaatcaata
     7621 gttatttcta aaaatcagcc gaaagaagtt gtttacagtg aactttttcc tcagttgaaa
     7681 tcactgctgg aaggcgaaac ggatttaatt gccaatctgg caaacgtggc tgcggcgctg
     7741 aaagaagttt tcggctggtg gtgggtagga ttttatctgg taaagggaaa tgagctggtt
     7801 ctcggacctt ttcaggggcc ggttgcctgt acgcggattg cattcggacg aggcgtttgc
     7861 ggaatggcat ggaaagaaaa acgctctgtt ttagtgccgg atgtgaatca gtttcccgga
     7921 catatcgcct gcagcagcgc ttcggtttcg gagattgttg tgccggtttt taacgaaaat
     7981 aatgaagttt tcgccgtact ggatgtggac agcgagcgat acgacgtgct ggacgaagtg
     8041 gatgtggagt ggttggagaa aatagtcctt atgaagaatt taaaatttac aatttaacga
     8101 tttacaattg ggattcacga tgcttataaa aaaaatactc ttattcatga gtgtttttcc
     8161 aatttaacga tctctttaag tcccccttgg gggatttagg gggcagtaaa tgacttccag
     8221 aaagcgcgcc atttttccaa atcgccggcc tcctgaattt tatcgtgatt gatggcaagt
     8281 aaagattttt ctccgcctgc tttggtttgg atattcacgc tcacattcga cttatcttcc
     8341 aggccacagc gccaatagcg ccatttttca gtttggctga ttgtaggttc gcgggtaatt
     8401 tttactctgt tgaattcggt cattccttct acccttttca accatttttc cagggcatca
     8461 tccatatttc cgccaatggt tttacttact gtaacctgaa aatcaccatc gcaggtttga
     8521 ccgggaattc gtcttccaat gtgctgctcg taagccacag ttattccctg gctccaccag
     8581 gcaccggctc cgtgagtatt aacccttaca gctatttcat catgtggaag ttctctggct
     8641 ttaatgcttt ccagaaattc cagccagtat tcccagcttt ggccggtgcc ggtttcaagt
     8701 gctttgacgt tggagggttt ggtcataaag cgaatttaag attcacgatt cacgattcac
     8761 gatttaagat ttacgattga aaaaaaattg cgatgattgg ctgctccgat acttcttttt
     8821 cgtcttaatt cgtcttaatt cgtgtaattc gtacaaatca acatatacta cacccctcac
     8881 acttgcttaa ttcgccgcgc aaccgtttct ctaccagcat tttaattcta tctttcagtg
     8941 cgtcaatccg gatattttct ataacatcgg ctgtaaacgc atacatcaaa agcatacggg
     9001 cttctttctc tgaaatgccc cgctgacgca agtagaacat cgctttttcg tccagctgac
     9061 caatcgtagc gccgtgcgaa catttcacat catcagcata gatttcaagt tgtggtttgg
     9121 tacgcatttt tgccgtgcgg ttaagcagaa tgtttttatt attctgatat gcaagcgttt
     9181 tctgcgcgtc ttttgccacg taaagtcttc cggtaaatcc acctttggct tcttcgtcca
     9241 gaatgtattt gaaaagttcg ttgctgtgcg aatttggctt gttgtgaagg atagatgtgt
     9301 aattatcaat cacctgttta ccgtcagaca gcgccattcc gcaaagatac gtttcgcagt
     9361 tttcgccatc caaatcgatt tccacagtgt ttcgtgtcat gccgttgtga agcgtcaaaa
     9421 gatttcccaa cacattggaa ttttgcattt ggtcaatcaa tagcgttgaa acgtcggtcg
     9481 tttttcgact tacgttttcc agtttataat gttcataaaa tgcattttct cccacaaaaa
     9541 cttccgtcaa ccgattggaa aaaaatcgca catcatccac cgtatgatca cacacaagca
     9601 cctgagcctt agctccttgc tccacgatta cgagattgtg actgttcgcc ataaaatcga
     9661 catccgaacg catgatgttg ataatctgaa tgggatgatc aagcaacacg tttttaggaa
     9721 cataaatgaa aaatccgtct tgcgcaaaag ctccgttgaa agcaatcatg ccgtctacgt
     9781 gtttttcagc cagtttggaa atgtgctttt caaccaattc cggatattct tctgaagctt
     9841 tccgtaagct ggtgattacc acgccttccg gcaatgattt gtttttttcg taatttacag
     9901 gataaaacgc atcattcacc acaaaataaa gataagccga aattcccggc acgtcgcact
     9961 tgaacacttc atacgggttt acgggaatgt tcattctttt gatattcaga ccgtaatcta
    10021 cggtcagcgg ctcacggagg tcggtgtatt tataattttc aacacgcggt gtaggaaatc
    10081 ccaattcacg aaatttctca aaagctttat cgcgaccgat attcaatgcg gacgccgaat
    10141 gcttgcggat ggtttccgaa tgttgctcgt aaagctcgat atattgctgt tcaaggacaa
    10201 tcgcttcttt tttcggagtt gcttgttcct gaacgtttgt attagtattt gccattttat
    10261 tgatacaaaa ttttcaattt atttcttcct tttccggaag tttttgtaat ttttgaaagc
    10321 ggaaatttcg tttttttttg gcttcgccaa aattcggaca agaatgtcta aattcctgaa
    10381 actgcacttc cgacttccat ctcccaactt tatctacttc aaccaatcgt atcctttctc
    10441 ttccagttcc attgccaacg atttatcacc ggatttgatg atttttccgt tgtgaagcac
    10501 gtgtacgaaa tcaggaataa tataatccaa aagtcgctga tagtgcgtga ttacgatgct
    10561 agcattttcg ggagttttca gcttgtttac gccgttggct acaatgcgaa gagcgtcaat
    10621 atccaaaccg ctgtcagttt catccagaat tgccaatttt ggttccagca tagccatttg
    10681 gaaaatctcg ttccgttttt tctcaccacc ggaaaaacct tcattcacag agcgattggt
    10741 caactggctg tccatttcca ccagttcttt tttctcacgc atcaaacgaa gaaattcggt
    10801 ggcagaaaca ggttcctgac cacgatattt acgttgttcg ttgatggcag cccgcataaa
    10861 attgaccatg ctcacacccg gtatttccac cg
//
LOCUS       DAORKP010000071        10857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_696_length_10857_cov_9.356230, whole genome shotgun sequence.
ACCESSION   DAORKP010000071 DAORKP010000000
VERSION     DAORKP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_10857_cov_9.356230"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLN75_05405"
     CDS             complement(<1..317)
                     /locus_tag="PLN75_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRG03587.1"
                     /translation="MVKDQIILGIDPGTTVMGYGIIRVSKNQPEMMAMGVLELRKYID
                     HYHKLQKIFSRTLSLVDEYKPDSLAIEAPFFGKNVQSMLKLGRAQGAAIAAALYRDLP
                     VYE"
     gene            complement(321..623)
                     /locus_tag="PLN75_05410"
     CDS             complement(321..623)
                     /locus_tag="PLN75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRG03588.1"
                     /translation="MLIYNTTYLIADRKFGPWIKWLQEIHIPFMLSCGFSNPLAAKIL
                     TADSEQEGTSIAVQFQIQNFQLLKIWDEENAEAILNDLSERFGSEVLSFATVMEML"
     gene            704..777
                     /locus_tag="PLN75_05415"
     tRNA            704..777
                     /locus_tag="PLN75_05415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:738..740,aa:Ala,seq:ggc)
     gene            complement(849..1271)
                     /locus_tag="PLN75_05420"
     CDS             complement(849..1271)
                     /locus_tag="PLN75_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03589.1"
                     /translation="MKKLLILSLGLLLMYSCSNNPQSMAEKGVTKYLKNSIAKYEPVS
                     FGALDTITVGEDPAYIAARDSMKIYMDGLKETADQFKLADNQKNAKRLKAVMADFEKF
                     YEGKKFKINHKYKANTAEGKNEEIDKDFFLNSMYEVVE"
     gene            complement(1397..3691)
                     /gene="rpsA"
                     /locus_tag="PLN75_05425"
     CDS             complement(1397..3691)
                     /gene="rpsA"
                     /locus_tag="PLN75_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRG03590.1"
                     /translation="MEEKEKLAEEVAQTPEAVEVTPETAAEATPEAVEVTPETVAEAT
                     PEAEEIQPAEVVEAVEESVEEVIEEAPSEKKEKKQKVVFEKPAGPDAGFDWDSYERGD
                     VLSGKSHDELEKMYDETLNAIKDHEVVEGTVISINKREVVVNVGFKSDGIVPMSEFRY
                     NPELKVGDKVEVYIESQEDKKGQLLLSHKQARATRSWDRVNSALESQEVVKGFVKCRT
                     KGGMIVDVFGIEAFLPGSQIDVKPIRDYDVFVNKTMEFKVIKINQEFKNVVVSHKALI
                     EEEIEQQKKEIISHLQKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVSHPE
                     EVVTLDQKLNVVILDFDDEKKRIALGLKQLSPHPWDALSADLKVGDKVNGKVVVMADY
                     GAFVEIAPGVEGLIHVSEMSWSQHLRSAQDFLKVGDEVESVILTLDRDERKMSLGIKQ
                     LKSDPWNNIEERYPLGSKHNAKVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPS
                     EFTQIGADIEVQVLEIDKDNRRLSLGHKQLEENPWDALESLFSVDSIHDGIIVEMMDK
                     GAVVSLPYGVEGFVTPKHLVKEDGSQAQVEEKLQFKVIEFNKDAKRIILSHSRIHEEA
                     KRAEKSAEGGDETKKTAKRVKKEEVSAPAIEKTTLGDIQQLADLKDQMVNEAAVKLAA
                     KEAKKVKAAKEAETEKEAQTVKAEKPVKTVAEVTVEKEEKPETEVKADVTVAEVVEAK
                     EVPANAEKTPKAEKKEKPAKEKEA"
     gene            complement(4072..5535)
                     /locus_tag="PLN75_05430"
     CDS             complement(4072..5535)
                     /locus_tag="PLN75_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRG03591.1"
                     /translation="MFKDLYLRKSLASIEAQAHENSLRKHLNVTNIILLGIGCIIGAG
                     IFVLTGTAAALHAGPAVSVSFLVSALGCLLAGLCYAEFASMLPVSGSAYTYGYATMGE
                     FIAWIIGWDLILEYLFGSATVAVGWSGYVVSFLDDIGLHLPAQICQSPFAYDINGWHH
                     TGAIINFPAVFIIALMTTLLVIGIRESARFNNVIVMIKVAVILLFIGFGISYINVDNW
                     TPFIPENTGKFSEFGLTGVLTGAAVVFFAYIGFDAVSTTAQEAINPQRDMPKGILISL
                     AICTLLYVAVSLVMTGIVNYTELNVPAPIAVAIDATGQGLAWLRPVIKIGAIAGLSSV
                     VMVLLLGQSRIFYSMAHDGLLWKSFARTHPKFKTPYITSIVTGCFAALFAGLFPIGLL
                     GELVSIGTLLAFVIVSVGLIILRYKEPDAPRTFRTPWVPFVPVMGALICLAQMAALPK
                     DTWLRLIGWMVIGFAIYFTYGRKHSIVRQEAKKNKLM"
     gene            5786..6661
                     /locus_tag="PLN75_05435"
     CDS             5786..6661
                     /locus_tag="PLN75_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRG03592.1"
                     /translation="MKLDFDKFIVKPGSKVKLKDYDTGYAAGLKKKKGVKELQNNIEE
                     LAVLQEKFWADNRYSMLIILQAPDAAGKDGAIRHVMSGLNPQSCRVHSYKAPSREELD
                     HDYLWRHYKDLPERGMIEIFNRSHYENVLATKVNPEYLMSENIPGIDSPEKADKKFWN
                     ERYEQINNFEKHIYQNGTRIVKIFLHLSKEEQKERLLARLNEKEKNWKFSSDDLKSRA
                     KWDEFQAAYEEMMEKTSTEYAPWYVVPADKKFFARVAIGEIIVEHLKKLDLQYPAGET
                     SGELQKAKEMLMNEV"
     gene            complement(6740..7900)
                     /gene="dnaJ"
                     /locus_tag="PLN75_05440"
     CDS             complement(6740..7900)
                     /gene="dnaJ"
                     /locus_tag="PLN75_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRG03593.1"
                     /translation="MAKRDYYEVLGVSKTATPDEIKKAYRQKAIQYHPDKNPGDKASE
                     EKFKEAAEAYEVLSDQQKRQRYDQFGHAGVGSSAAGGGFGGGGMSMEDIFAHFGDIFG
                     GHFGGFGGFSGFGGSQRGGQRVRRGSDLRVKVKLDLSEIANGVEKKIKVKKYVSCKHC
                     NGTGAESGSGTTTCTTCNGNGRVTRVQQTILGQMQTTSECPTCQGEGKIIKDKCSHCN
                     GDGIVREEEVININIPAGVMEGMQLSVGGKGNAARRGGVNGDLLVVIEEEHHPELIRD
                     DNDLIYNLLLSVPVATLGGSVEVPTVDGKVKVSIPAGTQPGKVLRLRGKGLPSVNSYG
                     KGDLLVNIGVYIPENLSRDEKQTMENLNKSNNFKPNSSAAKGFFSHFRNMFE"
     gene            complement(7906..8487)
                     /locus_tag="PLN75_05445"
     CDS             complement(7906..8487)
                     /locus_tag="PLN75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRG03594.1"
                     /translation="MKNKKHAPEKETENKDVLMPENVENAEQTENNKENITEETDKIA
                     EEIAKLNQKCEELNDKNLRLMAEFDNFRKRTLREKADLIKTAGEKVFVNILPLIDDFE
                     RAIQAMDSTEDVLAVKEGVDLIYAKFISFLNANGVKAIATENEPFDVDVHEAVTMFPA
                     PSEEMKGKIIDCLSKGYMLNDKVIRYAKVVVGE"
     gene            complement(8649..10646)
                     /locus_tag="PLN75_05450"
     CDS             complement(8649..10646)
                     /locus_tag="PLN75_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRG03595.1"
                     /translation="MKSKLILLTILSFLSFQLFSQIYDPVSWSVEKKVTGKTTADLII
                     KATIEKGWHLYGLNIPPDGPIATRISVENLENAKKEGTLQAKSKLIEAFDPNFDMKLN
                     WYANEAVFVQKVSSPDPNAIYAKVLVNFMVCNDESCLPPTEEELEVGKPGIKVSEVKE
                     ETPLTASAVAGIDSVLKPDSALLVPPALSDSTAKADLWTPVIDQLRTFGENASGGGMP
                     SLWWIFIMGMLGGFVALFTPCVWPIIPMTVSFFLKRSENKARGRRDAATYGLAIILIY
                     VGLGLLITVIFGASALNSLSTNALFNLIFFALLVVFAISFFGAFEITLPASWSTKMDM
                     KADSTAGILSILFMAFTLVLVSFSCTGPIIGTLLVQASVSGSILAPAVGMLGFAIALA
                     IPFSLFAFFPTLLKSMPKSGGWLNRVKVVLAFLELALALKFFSVADLAYGWGLLDRET
                     FLALWIVIFALLGFYLIGKLRFKHDDETKHTSVTGLFLGIISLAFSIYMIPGLWGAPL
                     KAVSAFSPPVITQDFNLYENDVHAKFTDYELGMNYAASVNKPVVVDFTGFGCVNCRKM
                     EVAVWTDPQVSNLLNNDYVLISLYVDDKTPLKEPYEITENGRTRKIKTIGDKWSYLQR
                     YKFGANAQPFYVMLDNQGKPMAKSYAFDENPANFVKWLERK"
BASE COUNT         2942 a         2478 c         2241 g         3196 t
ORIGIN      
        1 tattcatata ccggcagatc acggtacaaa gcagctgcaa tggctgctcc ctgagctctg
       61 ccaagtttga gcatggactg cacgttttta ccgaagaaag gggcttcgat agccaggcta
      121 tcgggtttgt attcatcgac aagcgagagt gtacggctga atattttctg aagtttgtga
      181 taatgatcga tgtatttcct gagttcaagc acacccatgg ccatcatctc gggctgattt
      241 ttactcacgc ggataatacc gtaacccatt acggtggttc cggggtcgat tcctaagatt
      301 atctgatctt ttaccatggc ttacagcatt tccataacag tagcaaacga cagtacttcc
      361 gatccaaacc tttcagaaag atcattaaga atagcttctg cattttcctc atcccaaatc
      421 ttcaacagtt gaaaattctg aatctgaaac tgcacagcaa tggaagtgcc ttcctgctcg
      481 gaatcggctg tgagaatttt ggctgcgagc ggattgctga atccacagct gagcataaac
      541 ggaatgtgaa tttcttgaag ccatttgatc cagggaccga acttacggtc ggctatcaga
      601 taagtcgtgt tgtaaatcag catatttttt ttgatatatt ttcgaacaaa attacgaatt
      661 tatttccgaa aatgattatc tttgcatttc aatttcaaaa aacgggggtt tagctcatct
      721 ggctagagcg cgacactggc agtgtcgagg tgaccggttc gagtccggta atctccaccc
      781 ggtaaaaacg aaggaaggat taacttgctg ctaatccttc cttcattttt ttttgatatt
      841 tattggaatt attctaccac ttcgtacata ctgttcagga aaaaatcctt atcgatttct
      901 tcatttttgc cttcagctgt attggctttg tatttatgat tgattttgaa ttttttacct
      961 tcgtagaatt tctcaaagtc agccatcact gcttttaatc ttttggcatt tttctgatta
     1021 tcagcaagtt tgaactgatc tgccgtttcc ttgagtccat ccatatagat tttcatcgaa
     1081 tctcttgcgg ctatgtaagc cggatcttca cccacggtta tggtatccaa tgctccgaac
     1141 gaaacaggct catattttgc aatgctgttt ttcaggtact ttgttacacc cttttcggcc
     1201 atactttgcg gattgttgct gcaggaatac ataagcagca atccaagtga taaaattagt
     1261 aattttttca tatctgtttg tttttagtgt tgaataagta tgcaaacata catttttttt
     1321 tacaaattat caaagaaaat caaaaaaaaa gctgtacaat ttattgcaca gctttttttt
     1381 tgattaattg gataaattat gcttctttct cttttgcagg tttctctttc ttttctgctt
     1441 ttggagtttt ttctgcatta gccggtacct ctttagcctc aaccacttca gctaccgtta
     1501 cgtctgcttt tacttcagtt tcaggtttct cttctttttc aaccgttact tctgctacag
     1561 tttttacagg tttttcagct tttactgttt gagcttcctt ttctgtttca gcttctttgg
     1621 cagcttttac tttcttagct tctttggcag cgagtttcac ggcagcttca ttaaccattt
     1681 gatctttcag atcagccagt tgctgaatgt cgcctaaggt agttttttca attgcaggag
     1741 ccgaaacttc ttctttctta acccgttttg cggttttctt agtttcatcg ccaccttctg
     1801 ctgatttttc agctcgttta gcttcttcgt gaatacggct gtgtgaaaga atgatgcgct
     1861 ttgcatcttt attgaactcg ataaccttga actgaagttt ttcttcaacc tgtgcttgtg
     1921 atccgtcttc tttcaccagg tgtttcggag ttacgaaacc ttctacgccg taaggcaacg
     1981 aaacgacagc ccctttgtcc atcatttcaa cgatgattcc atcgtgaatt gagtcaactg
     2041 aaaacaggct ttcaagagca tcccatggat tttcttccaa ctgtttgtgt ccaagactca
     2101 accggcggtt gtctttgtca atttcgagca cttgtacttc gatatcggca ccaatctggg
     2161 taaattcgga cgggtgtttg attttctttg tccagctcag gtctgagatg tgaatcaacc
     2221 catctacacc ttcttctatt tcaacgaaaa ctccaaagtt ggtaaagttg cgcactttag
     2281 cgttgtgttt gcttcccagc ggatatcttt cttcgatatt attccatggg tcggatttca
     2341 gctgcttgat gcccagtgac attttacgtt catcgcggtc aagtgtgaga attactgatt
     2401 ccacttcgtc gcctaccttg aggaagtcct gtgcagagcg aaggtgttgt gaccagctca
     2461 tttcagatac gtgaatcaga ccttctactc cgggtgcaat ttcaacgaat gctccgtaat
     2521 cggccatcac cactactttt ccatttactt tgtcgcctac tttcagatct gcactgagag
     2581 catcccacgg atgtggtgaa agttgtttaa gaccaagagc gatgcgtttc ttttcatcat
     2641 cgaaatcgag gataaccacg ttgagtttct ggtcgagtgt aaccacttct tcaggatgcg
     2701 atacgcgtcc ccagcttaag tctgtaatgt gaatcagccc atctacgccg ccaaggtcga
     2761 tgaatactcc gtaagaggtg atattcttaa ctgttccttc gagaacctga cctttctgaa
     2821 gatgactgat aatttctttt ttctgttgtt cgatttcttc ttcgataagt gctttgtgag
     2881 aaacgacaac atttttgaat tcctggttga ttttaatcac cttgaattcc atcgttttat
     2941 taacgaatac gtcgtagtcg cggatcggtt tcacgtcaat ctgtgagccc ggcaggaatg
     3001 cttcgattcc gaatacatct acaatcatac cgccttttgt gcgacatttt acaaagcctt
     3061 tcaccacttc ctggctttcg agcgctgagt tcacacgatc ccagctgcgt gtagcacgag
     3121 cctgtttgtg cgaaagaagc agctgccctt ttttgtcttc ctggctttcg atgtagactt
     3181 ctaccttatc gcccactttg agttcggggt tgtagcggaa ttcgctcatc ggtacgatgc
     3241 cgtctgattt gaaacctaca ttgaccacta cttcgcgttt gttgatagag ataacggtac
     3301 cttccactac ttcgtgatct ttgatggcat tgagtgtttc atcatacatt ttttcaagct
     3361 catcgtgaga ttttccggaa agtacatctc ctctttcata agaatcccag tcaaatccgg
     3421 cgtccggacc tgcaggtttt tcgaacacca ctttttgctt tttctctttt ttttctgaag
     3481 gagcttcttc tatcacttct tctactgatt cttctactgc ttctacaact tctgcgggtt
     3541 ggatttcttc tgcttcgggt gttgcttcag caacagtttc cggagttacc tccacagctt
     3601 cgggagtagc ttcagcagca gtttcaggag ttacctccac agcttcggga gtttgagcca
     3661 cttcttcagc taatttttct ttttcttcca tttctgaaat ttttgttttt ttactaataa
     3721 atagtaggtt agacattatg ttgattaaaa aaaaacgtgg gtgcaaaggt acaaaaatta
     3781 aatagattat atgcaatatc tttacttttt tcttaggggt taaaagcctg tttttcacca
     3841 aagaaacaaa aaaaacatga tttctgagga aatatttctt ttcacaaaaa atacggcgat
     3901 aaaaaaacac acggaataac agagtgataa tttttattca ttttttatcg aaattgagtt
     3961 aatgtatact acacagaggc gtctccacat tttgagaata caaaatgtgg agacgcgaat
     4021 cagtaggtct gaaatagtgt gaacggaaaa tgtaccgcaa gtgattattc gctacattaa
     4081 tttattcttt ttggcttcct gacgtactat gctgtgtttt cggccgtaag tgaaatagat
     4141 tgcaaagccg attaccatcc accctatgag ccgcagccag gtatctttgg gcaatgcggc
     4201 catttgagcc agacagatta aagcacccat aactggcacg aaaggcaccc agggagtgcg
     4261 gaaagtgcgg ggagcatcgg gttctttgta ccggagaatt atcaatccga cactaactat
     4321 tacgaatgcg agcagggtgc cgatggatac cagttcgccc agcaacccga tggggaataa
     4381 accggcaaac aaagcggcaa agcatcccgt aacgatactg gtgatgtagg gagttttgaa
     4441 tttcggatga gtccgggcaa agcttttcca gagcaatcca tcatgcgcca tggagtaaaa
     4501 tatgcgggac tgtccgagta acagcaccat gactacggag ctcagaccgg caattgcacc
     4561 tattttgatc acaggtcgca accacgcaag tccctgtccg gtagcatcaa tggccacagc
     4621 tatcggtgcc gggacgttta attcggtata attgacaatg ccggtcatca ccagactgac
     4681 agccacatac aacaaggtac aaatagcgag tgaaataaga atccccttcg gcatatcgcg
     4741 ctgcggatta atggcctcct gtgcagtggt ggaaacagca tcaaaaccga tgtaagcaaa
     4801 gaaaacaaca gcagctccgg ttaatactcc cgttaggccg aattcggaaa attttccggt
     4861 attttcggga ataaaaggtg tccagttatc tacattgatg tatgaaatac caaatccgat
     4921 aaacagcaaa atgacagcga ctttaatcat cacaatgaca ttattaaaac gagccgactc
     4981 ccggatgccg ataaccagca aggtagtcat cagagctatg ataaacacag ccgggaagtt
     5041 gataatagca ccggtgtgat gccacccgtt gatatcataa gcaaacgggc tctgacaaat
     5101 ctgtgccgga agatgcaagc caatgtcgtc cagaaaactc accacataac ccgaccatcc
     5161 taccgccact gtggctgagc cgaaaaggta ttcgaggatc aggtcccagc cgataatcca
     5221 ggcgataaac tcacccatag tggcatatcc gtaggtataa gcactgcctg ataccggaag
     5281 catggaggca aactcggcgt aacacaagcc cgctaacaaa catcctaaag ctgaaaccag
     5341 aaaagaaact gatacagcag gaccggcatg aagcgctgcc gcagtgcctg tgagtacaaa
     5401 aattccggct ccgatgatac atccaatccc cagaagaatg atattggtaa cattgaggtg
     5461 ttttctcaaa ctgttttcgt gagcctgtgc ctctatgctg gccagacttt tgcgcaggta
     5521 taaatctttg aacataagct gtcagatcgg tgaaaatggt atgattatta cttacttttt
     5581 ggaaatttat caacgcaaaa ataatcaaat ttatgattta tttactaaaa aatgtaattt
     5641 ttattcacat catcgttgaa atattccggt aaaagaatcg atcggactga aatgaaaaaa
     5701 gaaaacaggt taaactattt gatttcaaat ttgttaacaa taaataaatc tacattacgt
     5761 tcatcttaaa aaaatcatta ccatcatgaa actcgatttt gataaattca ttgtaaaacc
     5821 gggcagtaaa gtaaaactga aagattacga taccggatac gctgccggac tgaagaaaaa
     5881 aaagggtgta aaagaattac aaaacaacat tgaggaactg gcagtactgc aggaaaaatt
     5941 ctgggcagac aaccgctact ccatgcttat catactgcag gctcccgacg ctgccggcaa
     6001 agacggtgcc atacggcatg tgatgtcggg gctgaatccg caaagctgta gggtgcacag
     6061 ctacaaggct ccatcgcgcg aggaacttga ccacgattac ctgtggcgcc attacaaaga
     6121 cctgcccgaa cggggaatga ttgaaatttt caaccgctcg cactacgaga atgtactggc
     6181 taccaaagtg aatcccgaat acctgatgag tgaaaacatt ccgggaatcg acagccccga
     6241 aaaagccgat aagaagttct ggaacgagcg ctacgagcaa atcaacaatt tcgagaaaca
     6301 tatctatcaa aacggcaccc gcatagtcaa gatttttctg cacctctcca aagaggaaca
     6361 aaaagagcgg ctgctggctc gactcaacga aaaagaaaaa aactggaaat tcagttccga
     6421 cgatctgaaa tcacgtgcta aatgggatga atttcaggct gcctacgaag aaatgatgga
     6481 aaaaacatcc accgaatatg ctccctggta tgtggtaccg gctgataaga agttctttgc
     6541 ccgggtggca atcggcgaaa tcatagtgga acatctgaaa aaactggatc tgcagtatcc
     6601 ggcaggtgaa acgtccgggg agcttcagaa ggcaaaagaa atgctgatga acgaggttta
     6661 gacaacatgt tttttttaaa aaaaaagcaa agggtgaaat gcaatatctc accctttgtg
     6721 tgtattattt tctgaaaaat tattcaaaca tattccggaa atgggagaaa aatcctttgg
     6781 cagccgatga gtttggtttg aaattattcg atttgttgag attttccatc gtttgttttt
     6841 catcacggct cagattttcg ggaatatata ctccgatatt gaccagcaaa tcgcccttgc
     6901 cgtagctgtt tacactcggt aaaccttttc cgcgcagacg aagcactttg ccgggttgag
     6961 taccggccgg aatgcttact ttcacttttc catctacggt gggcacttct accgatccgc
     7021 ccagtgtagc caccggaacc gaaagtaaca gattgtatat caaatcattg tcatcgcgaa
     7081 tcaattcagg atgatgttct tcttctatca ccacgagcag atcgccgttc acaccgccgc
     7141 gccgggcagc atttccctta ccgcctaccg aaagctgcat accttccatt acacccgccg
     7201 gtatattgat gttgatcact tcttcttcac gtactattcc gtcgccgttg cagtgagagc
     7261 atttatcctt gataatcttc ccttcgccct ggcaggtcgg acattcactg gtggtctgca
     7321 tctgtcccag aatagtttgc tgcacacggg taacacgtcc gttaccgtta caggtggtac
     7381 acgtagtcgt acccgatccg gattcagctc ctgtgccgtt acagtgctta caggaaacgt
     7441 atttcttgac tttaattttt ttctctactc cattggcaat ttccgacagg tccagtttga
     7501 ccttcacacg caaatcggaa ccgcgacgaa cgcgctgccc acctctctga gagccgccaa
     7561 atccactgaa accgccaaat ccgccaaaat gtcctccaaa gatatcgcca aaatgagcaa
     7621 aaatatcttc catgctcatt ccgcctccgc cgaaacctcc tccagctgca ctgctgccta
     7681 cgcccgcatg accaaactgg tcgtaacgct gacgtttctg ctgatcgctc aaaacttcgt
     7741 aagcctcggc agcttccttg aatttttctt cagaagcctt atcacccgga tttttatcgg
     7801 gatgatactg aatggctttc tgacggtagg ctttttttat ctcgtctggc gtagctgttt
     7861 tggatacacc caatacttcg taataatctc ttttggccat atctcttatt ctccaactac
     7921 aactttagca taacggatta ccttatcatt cagcatgtat ccttttgaca gacagtcaat
     7981 tattttacct ttcatctctt cggacggtgc cggaaacatc gtaactgctt cgtgtacatc
     8041 tacatcaaac ggctcatttt cagtagcaat agctttcacg ccgttggcat tcagaaaact
     8101 gataaatttg gcgtaaatca aatccacacc ctctttcaca gccagcacgt cttctgttga
     8161 gtccatggct tgtatagctc tttcgaaatc atcgatcagc ggaagaatat tgacaaacac
     8221 cttttcgccg gcagttttta tcaaatcggc cttttcacgg agagtacgtt ttctgaaatt
     8281 gtcaaattcg gccatcaacc gtagattttt atcgtttaac tcttcacatt tctggttcag
     8341 ttttgcaatt tcttctgcaa ttttgtcagt ttcttcggtg atgttctcct tattgttttc
     8401 agtttgttct gcattctcca cattttccgg catcaaaacg tctttgtttt ctgtttcttt
     8461 ttcaggagcg tgttttttat ttttcatatc tgaatttgtc tgtattgtgt attttattga
     8521 atttattaat tgttgatgaa gcaataagca gtctgtttat tacaataaac ctgccaaaac
     8581 atccggactt cattttttgt cagaaatcgg gacattttgg caggttatca ttttttcagt
     8641 gaattggact attttctttc aagccatttc acaaaattag ccggattttc gtcgaaagca
     8701 taagattttg ccattggttt gccttggttg tcgagcatga cataaaacgg ctgtgcgttt
     8761 gctccgaatt tatatcgctg caggtagctc catttgtcgc ctatggtttt tatctttcga
     8821 gtccgtccgt tttcggtgat ttcgtaaggc tctttcagcg gtgttttatc atccacatag
     8881 agcgaaatca gcacatagtc gttgttgagc agattgctta cctgcggatc tgtccatacg
     8941 gccacttcca ttttacggca gttgacacat ccgaaaccgg taaaatcaac gactacaggc
     9001 ttgttgactg aagctgcata attcattccg agttcataat cggtaaattt ggcatgcaca
     9061 tcattttcat acagattgaa atcctgtgtg attaccggcg gcgagaatgc gcttaccgct
     9121 ttcaatgggg caccccacaa tccgggaatc atgtaaatag aaaatgccag cgaaatgatt
     9181 cctaaaaaca agcctgtgac tgatgtgtgt ttggtttcat catcgtgctt gaagcgcagt
     9241 tttccgatga ggtaaaagcc taacaaagca aatattacaa tccacaacgc caggaatgtc
     9301 tcacggtcga ggagtcccca gccataagcc aaatcggcaa cagagaagaa cttgagcgcc
     9361 agcgccagct ccaggaatgc cagcaccacc ttcacgcggt tgagccatcc gcccgatttc
     9421 ggcatcgact tgagcaaggt tgggaagaaa gcaaaaaggc tgaaaggaat ggccagtgca
     9481 atggcaaatc cgagcatacc gacggcagga gccagtatcg acccactcac cgaagcctgc
     9541 acaagcaagg tgccgataat aggaccggtg caggaaaacg aaaccagtac gagtgtaaaa
     9601 gccatgaaaa gtatgctcag aatgcccgct gtggagtcgg ctttcatgtc cattttggtg
     9661 gaccaggaag ccggaagggt aatttcgaag gctccgaaga acgagatggc aaacaccacg
     9721 agcaaagcga agaatatcag attaaagaga gcgttggtag agaggctgtt gagagcgctt
     9781 gctccgaaaa taacggtaat gaggagtccc agacctacat aaatgagaat aatggccaag
     9841 ccgtaggtgg cagcatcgcg gcgtccgcgg gctttgttct ccgaccgttt cagaaagaaa
     9901 cttactgtca tcggtatgat aggccatacg cacggtgtaa acaaggctac aaatccgccg
     9961 agcatcccca tgataaaaat ccaccagagc gatggcattc ctccaccgct cgcgttttcg
    10021 ccaaaagttc gcaactgatc gatgacaggt gtccaaagat ctgccttcgc agtagaatct
    10081 gacaacgccg gcggaacaag caaggcagaa tcaggcttca gcaccgaatc gattcctgca
    10141 actgcagatg cagtcaatgg cgtctcttct ttcacttcag agacttttat tcccggtttt
    10201 cccacctcga gttcttcttc ggtaggaggc agacagcttt catcattaca caccatgaaa
    10261 ttcaccaaca ctttggcata aatggcattc ggatcgggtg aagacacttt ctgcacaaat
    10321 accgcctcat tagcatacca gttcagtttc atatcgaaat tgggatcaaa cgcttctatc
    10381 aactttgatt ttgcctgaag tgtaccctct tttttggcat tttcgaggtt ctcaacagaa
    10441 attctggtgg cgataggacc atcgggagga atgttcagtc catacaaatg ccatcctttt
    10501 tctatggtgg ctttgataat cagatcggct gtggttttac cggtcacctt cttttcgacc
    10561 gaccacgaca ccgggtcata tatctgactg aacaattgaa aggacaaaaa acttaaaatg
    10621 gtaagcagaa tgagttttga cttcatcgtt tcgttttttt atatttacaa aattgggtgc
    10681 aaatttagga ataatccgac aataatataa cctaagtatc actattttca gttatttttc
    10741 agaaagataa caagtaataa tgcatttggt tcatgcccga aagtttttcc ggcagataaa
    10801 ccctacctcg tgagcataaa cttgaaactc agtccgaaat tggtggaaga aaccggg
//
LOCUS       DAORKP010000072        10850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_698_length_10850_cov_9.892358, whole genome shotgun sequence.
ACCESSION   DAORKP010000072 DAORKP010000000
VERSION     DAORKP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_10850_cov_9.892358"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLN75_05455"
     CDS             <1..1416
                     /locus_tag="PLN75_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG03596.1"
                     /translation="ITDEVMADIVSKLDPHSVYIPAKDLEAVNSELEGSFSGIGVQFN
                     IQNDTVMIVSVISGGPSEKVGLMAGDRIIRVNDTIFAGAGVVSNEKVMRKLRGPKGTE
                     VKLGVKRHGTREILSYTIKRGEIPVNSVTVAYIIEPGVGFIGLNNNFGNNTYDEFLHA
                     IAELRAKGARKFIIDLRENSGGLMDVAIRMINEFLPAGQMIVYSEGKAYPRADAKADG
                     TGSCINYPLAVLIDEFSASASEIFAGAIQDNDRGLIIGRRSFGKGLVQTQIPFSDGSA
                     VRLTVARYFTPSGRSIQKPYERGKGDDYQMDLVKRFEHGEFDTKDSIHVADSLKFKTL
                     KGRTVYGGGGIMPDIFVPRDTSKYSPYLTKVVNYGYIYQYAFQYTDKNRTALSKFKSW
                     QEMDKYLSGQNLLNEFVSFASSKGVPANQREISVSESLLRNQIKSYIVRNMLGDSQFF
                     PMLNKEDETVKKALEELKSQK"
     gene            complement(1459..2193)
                     /locus_tag="PLN75_05460"
     CDS             complement(1459..2193)
                     /locus_tag="PLN75_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRG03597.1"
                     /translation="MNKISETILSNSCKEKKMLAVLLDPEKCREKMLVSVLSAFEKNL
                     PDFIFVGGSSKSCPTDDLFDALHDVPVPKILFPGDASQFSPEADALLFLSLISGRNPD
                     YLIGQHVLSALEIKQSGIETIPTGYILIDGGNHSAVQRVSQTIPIPAHSVDECVSTAV
                     AGELLGMKMIYLEAGSGAENPVSADIIAAVKRNLNIPLLVGGGIRTTGQLTSALSAGA
                     DLIVVGNVFESQTDKIAEFIHCVKSH"
     gene            complement(2186..2818)
                     /locus_tag="PLN75_05465"
     CDS             complement(2186..2818)
                     /locus_tag="PLN75_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRG03598.1"
                     /translation="MNDNHISTHYGSRICLLENDMTVDELIQSFNQPELYLPEMEKIN
                     SEHRRREYLGLRLALKQCLNGEEKTISHTPDGKPVLTDNSYKISFSHCKNWISVIANP
                     TYEVGIDIERPTDRIEKIHTRFLGNEELEEYLKHRELNYLKVVWSTKEALYKIIGEKA
                     YNFARKIRILPFELREEGELKALFQETNKEYLVNYRLTEKYTLAYCTDNE"
     gene            complement(2870..3454)
                     /gene="gldD"
                     /locus_tag="PLN75_05470"
     CDS             complement(2870..3454)
                     /gene="gldD"
                     /locus_tag="PLN75_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRG03599.1"
                     /translation="MKIDTLKLIFSLVLTGLLISCADNPTPRPYGYFRVDLPPHQYVR
                     YDSAALPYRFDKPVIASIKPVTEKGENYWIDINYSILKASIYCSYIPVRANLFNLLED
                     SRKIVYKHTVRADGISETPFENPGRKVYGILYQLSGNTASPAQFVLTDSSKHFFRGAL
                     YFEQVPNKDSIAPMADYIRKDIIQLMESFEWKNN"
     gene            complement(3444..4775)
                     /gene="gldE"
                     /locus_tag="PLN75_05475"
     CDS             complement(3444..4775)
                     /gene="gldE"
                     /locus_tag="PLN75_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRG03600.1"
                     /translation="MLLNIFTDVYFTAPQWSDYVLLVLSMLFLAFSAFFSSSEVAYFT
                     LSPKNLAEMHESEEKSAKTTIKLLNSPQKLLATIMIGNSVANIAFVILSTTLIYSVFD
                     FELTPVWKFIAPVLFVSVTLLTFGQIMPKVYSSEYSTETVLKNSGIILALQNFFKPFV
                     DILAESKALVNSRLPKRSPNMISMDELSHALDLSDDKQEEDKEMLEGIIKFANIQVAD
                     VMTSRMDMVIVDIKLNFKQLMDVIIESGYSRLPVYAGTRDNIKGILFSKDLLPYLDKP
                     ETFRWQTLIRQPYYVPETKKIDDLLSEFQENRIHLALVVDEYGGTSGLVTLEDIIEEI
                     VGDISDEYDEEETLFQKIDNHTYIFEAKILLNDFFKAADIERELFDKITEEVETLAGM
                     ILEIKGEMPVKNEMIGFKHYVFEIIAVDNRRIKKVKLHIKNNPNKPADDED"
     gene            4967..5644
                     /gene="trmD"
                     /locus_tag="PLN75_05480"
     CDS             4967..5644
                     /gene="trmD"
                     /locus_tag="PLN75_05480"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRG03601.1"
                     /translation="MRIDIISAVPELLDSPLNHSILKRAKEKGLVEIFVHSLRDYTDD
                     KHRRVDDYAFGFGAGMVLQIEPVDNAISALKSARSYDEIIYTSPDGEKFTQKLANRYS
                     LAENLIILCGHYKGIDHRIREHLITREISVGDFVLTGGELPAAIMADAIVRLIPGVIG
                     DETSALSDSFQDNLLAPPVYTRPADYKGWKVPEILLSGHQARVEEWMHQQSVERTRLL
                     RPDLLEE"
     gene            5649..5909
                     /locus_tag="PLN75_05485"
     CDS             5649..5909
                     /locus_tag="PLN75_05485"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRG03602.1"
                     /translation="MNAFFTYLVVVNIVSAVLFTYDKVAAIRRKRRIPELVLHFSEVT
                     GGVFFVVMLMYLINHKNRKPDYYRITYVVLIVWTVIAGWFFT"
     gene            5936..6589
                     /locus_tag="PLN75_05490"
     CDS             5936..6589
                     /locus_tag="PLN75_05490"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRG03603.1"
                     /translation="MSGKKQSIESRLFHGVKEFRENDFEQNREVFKNLESSQHPHTLF
                     IGCSDSRVVPNLITSTLPGELFIIRNIANLVPYYKENHDTYVATSSAIEYALHVLKVE
                     NIIVCGHSNCGGCRALYSDRLQNQLPLTRKWLELAAPVKKLVKAKILNEKLKPCRRDM
                     LTEQLNVVEQINHLLTFPYIKERYDKGELNIMGWYYVIEKGEVYNYSHDNQVFERIE"
     gene            6651..6989
                     /locus_tag="PLN75_05495"
     CDS             6651..6989
                     /locus_tag="PLN75_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRG03604.1"
                     /translation="MAWIFLIIGGVFESLFSFSLGKISLTSGREMFLWILVFVFSVSL
                     SMYFLYKAIDSGLGVGVSYAVWAAIGAAGSVLMGIFLLAEPVSFWRIFFLTLLVVSVV
                     GLNLSSHPAS"
     gene            7028..7996
                     /gene="argC"
                     /locus_tag="PLN75_05500"
     CDS             7028..7996
                     /gene="argC"
                     /locus_tag="PLN75_05500"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRG03605.1"
                     /translation="MIKAGVIGGAGYTAGELIRILVHHPEVKIEFVNSTSNAGNPITA
                     VHGGLIGETGLIFTDELPLGETDVLFLCSAHGDSRKFLAAHEVPENVRIIDLSTDFRH
                     KRDDHDFVYGLPELNKNAIIHANRVANPGCFATTIQLALLPLAAAGLLTDDIHVNAIT
                     GSTGAGVKPSSTSHFSWRNNNISVYKAFEHQHLDEIFQSIHQLQPGFAKDLNFIPVRG
                     NFARGIFATTYTKSSLSAAEAEKIYRDFYNDAAFTHVVSENPDMKQVVNTNKAIVHVE
                     KHGEYLLIISAADNLLKGASGQAVQNMNLMFGLDEKTGLNLKSIGF"
     gene            complement(8208..10088)
                     /locus_tag="PLN75_05505"
     CDS             complement(8208..10088)
                     /locus_tag="PLN75_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRG03606.1"
                     /translation="MVNYIQVENLTKSFGDKLLFENISFGIADNQRVALIAKNGTGKS
                     TLLNILADRESPDSGHISFRKDLRIGYLEQSPELPSELSVIDACLQSDNQLMNTIARY
                     EHAMLHPENDTLSEVLAEMEMLKAWDYESRIKQILGKLKITNFEQKIGELSGGQLKRI
                     ALANVLISEPELLILDEPTNHLDLEMVEWLEDFLRRSSMALLMVTHDRYFLDRVCTNI
                     LELENHNLFSYSGNYSYYLEKRQERIDNFNAETTSAQNIYRRELEWIRSTPQARTGKS
                     KGRIDRFYDIEERAKQRLVNDNVKLAVKASYLGSKIFEAKYISKSYGDLKILDNFYYN
                     FGRFEKLGIVGKNGTGKTTFLKILLGQLKPDSGSVDIGDTVQFGYYSQEGLMFDEQMK
                     VIDIVRDIAEEVDLGNGKKMNVSQFLNYFLFSPETQHNYVYKLSGGEKRRLHLCTVLM
                     QNPNFLVLDEPTNDLDIVTLNILEDYLLTFKGCVIVVSHDRFFMDKVVDHLFVFEGDA
                     VVRDFPGNYTQYREASLNPSEGGTSKPASEKNQRKNTPTPNENRIEGKPRKMSFREKQ
                     EFEALEKEIPQLEAEKTEIEVRLSSGMLDNEAILAASERFGELTALIDEKTMRWLELS
                     EI"
     gene            complement(10173..10547)
                     /locus_tag="PLN75_05510"
     CDS             complement(10173..10547)
                     /locus_tag="PLN75_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03607.1"
                     /translation="MKNTITMGNDEFILYVRKNYNCSLTNDRIGKLVWSWILNNSKEI
                     DKTKDIERNVPCLWQNSRLRKTFGFRLPRTASQITFNRKLLPKLFSYLDQLGEIYRAD
                     EQFDFRKRIEEEEHKNMPELDF"
     gene            complement(10690..>10850)
                     /locus_tag="PLN75_05515"
     CDS             complement(10690..>10850)
                     /locus_tag="PLN75_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HRG03608.1"
                     /translation="AQDSIKNVEVLEYPELGMEAIWKIDVVDFPAFILVDDKGNDFFQ
                     LIKKQVIC"
BASE COUNT         2899 a         2271 c         2444 g         3236 t
ORIGIN      
        1 attaccgatg aagtgatggc tgatattgta tcaaaactcg atccgcattc ggtgtatatt
       61 ccggccaaag acctcgaagc cgtgaacagc gaactggaag gtagtttcag cggtattggc
      121 gtacagttca acattcagaa tgacacagtg atgattgttt cggtcatcag cggcggacct
      181 tcggaaaaag tgggattaat ggccggtgac cggataattc gggtaaatga tacaatattt
      241 gccggggcag gcgtggtgag caacgagaag gtgatgcgaa aacttcgcgg accgaaaggg
      301 actgaagtga aactcggcgt aaagcgtcac ggtaccagag aaatactttc atacaccatt
      361 aaaaggggag aaattcccgt taattcagta actgtagctt atattatcga accgggtgta
      421 ggttttatcg ggctgaacaa taatttcgga aacaatacgt acgacgaatt tctgcatgca
      481 attgccgaat tgcgagccaa gggtgcccgt aaatttatca tcgacctgcg cgaaaacagc
      541 ggcggactga tggatgtggc tatccggatg atcaacgaat tcttacccgc cgggcaaatg
      601 atcgtttact ccgagggaaa agcttacccg cgtgcggatg ccaaagccga cggaaccggc
      661 tcatgcatca actatccgct cgccgtgctg attgacgaat tcagcgcttc ggcaagcgaa
      721 atctttgcag gagctattca ggataacgac cgcggactaa ttatcggtcg tcgttcgttt
      781 ggaaaaggac ttgtgcagac tcagattcca ttttcggatg gttcggccgt gagacttacc
      841 gtagcacgtt attttacccc ctccggacga agtatccaga aaccttacga aaggggcaaa
      901 ggcgatgatt accaaatgga tctggttaaa cggttcgaac acggtgaatt tgacaccaaa
      961 gatagtattc atgtggctga ttcgctgaaa tttaaaaccc tgaaaggcag gacagtttac
     1021 ggtggcggcg gaattatgcc cgatatattc gtgccgcgag acacatccaa atattcacct
     1081 tatctgacta aagtggtgaa ttacggatac atttatcagt atgcatttca gtataccgac
     1141 aagaacagaa cggcactgag caaattcaaa agctggcagg aaatggacaa atacctgtcg
     1201 ggtcagaatt tgctgaatga attcgtttct ttcgcttcat caaagggagt tccggctaac
     1261 cagcgtgaaa taagtgtatc cgaaagtctg ctgcggaatc agataaaatc gtacatcgta
     1321 agaaatatgc tgggcgacag tcagtttttc cccatgttga ataaggagga cgaaacagtg
     1381 aaaaaagcct tagaagaatt gaaaagtcag aaataatcat tttcaaaaag aaaagcccga
     1441 tttcacgtcg ggcttttttt aatgagattt tacacagtga ataaattcag ctatcttatc
     1501 agtctggctt tcaaacacat tccccaccac tatcaaatcg gctccggcac ttaacgccga
     1561 ggtgagttgc ccggttgttc ttattccgcc tccaaccagg agcggaatgt ttagattgcg
     1621 cttcacggct gcgattatat cagccgaaac aggattttcc gctccgcttc ctgcttccag
     1681 atatatcatt ttcatgccca gcaattcgcc tgcaactgct gttgatacgc attcatccac
     1741 actgtgagca gggataggaa ttgtttgact aacgcgctga acagctgaat gatttccacc
     1801 atcaatgagt atgtagcctg tcgggattgt ttctataccc gattgcttga tttcaagagc
     1861 agaaagcacg tgctgcccga tcagataatc cggattgcgg cctgaaatga gcgagagaaa
     1921 aagcagtgca tcggcttccg gtgaaaattg ggaggcatcg cccggaaaaa gtattttcgg
     1981 aaccggtaca tcatgaagcg catcgaaaag atcgtcggtg gggcatgact tgctgcttcc
     2041 gcctacaaaa ataaagtccg gtaaattctt ctcaaaggcc gaaagtacac ttacgagcat
     2101 tttttcccgg catttttcag ggtcaaggag tactgccagc attttttttt ctttgcagga
     2161 attactcaga atggtttcgg atatcttatt cattatcagt gcagtaagct aatgtgtatt
     2221 tttcagtcaa gcggtagtta accagatatt ctttgtttgt ttcctgaaaa agcgctttga
     2281 gttcaccttc ctcccggagc tcgaaaggta gaatccgtat ttttcgggca aaattatagg
     2341 ctttctcccc gataatttta taaagggctt ccttggtgga ccagaccact ttcaggtaat
     2401 tcagttctct gtgtttcagg tattcttcca actcttcatt tcccagaaaa cgggtatgaa
     2461 ttttctcaat ccgatcggta ggtctttcaa tgtctatccc cacttcatac gttggattcg
     2521 caatcaccga aatccagttt ttgcaatgtg aaaaactgat tttatagctg ttgtcggtca
     2581 gcacgggctt gccgtcgggt gtatggctga tggttttttc ttctccgttc aggcattgtt
     2641 ttaatgccag ccgaagtccg agatattctc tcctgcgatg ttcggaattg attttttcca
     2701 tttcaggcag gtagagttcc ggctgattga acgattgtat cagttcatcc acagtcatat
     2761 cgttttcaag cagacaaatt ctgcttccgt aatgcgtgga tatgtgattg tcgttcattt
     2821 tttttaaaaa aaagttattg taaggatatt agcttgacag gatttggttt taattgtttt
     2881 tccattcaaa actttccatc agttgtatga tgtctttccg gatgtaatcg gccatcggtg
     2941 cgatagaatc cttgtttggt acctgctcaa agtaaagtgc tcctctgaaa aagtgcttcg
     3001 agctatcggt gagcacaaac tgtgccggcg atgccgtatt gcctgaaagc tggtataaaa
     3061 taccgtaaac ctttcgtccg ggattctcaa agggagtttc actgattcca tcggcacgaa
     3121 cggtgtgttt atacacaatt ttacggctat cttccagcag attgaacagg ttggccctga
     3181 ccggtatgta actgcaataa atacttgctt tgagaatgct gtagttgatg tcaatccagt
     3241 aattttctcc tttttcggtt acaggtttaa tgcttgcaat aaccggctta tcgaatctgt
     3301 agggcagggc agccgagtca taacgaacgt attgatgcgg aggcaaatct accctgaaat
     3361 atccgtaagg acgaggagtg ggattatctg cacagctgat taataatccg gtcagaacaa
     3421 gactaaagat taatttcagt gtatcaatct tcatcgtcgg ctggtttatt cggattgttt
     3481 ttgatatgca gtttcacttt cttgatccga cggttatcca cggctattat ttcaaaaaca
     3541 taatgtttga aaccaatcat ttcattttta accggcattt ctccctttat ttccagtatc
     3601 attccggcca gcgtttccac ctcttcggtg attttgtcaa atagttctct ttcaatgtcg
     3661 gctgccttga aaaaatcatt gagcagtatt ttagcttcaa aaatgtaggt gtgattgtca
     3721 attttctgaa acagtgtttc ttcttcgtcg tattcatcac tgatatcacc tacaatttct
     3781 tcgataatat cttccagtgt taccagccct gaagtcccgc cgtattcatc cactaccagc
     3841 gccaggtgga ttcggttttc ctgaaattcg ctcagcaaat catctatttt cttcgtttcg
     3901 ggcacatagt aaggctgccg aatcagtgtc tgccagcgga atgtctccgg tttgtcgagg
     3961 taggggagca ggtctttact gaaaagaatg cctttgatgt tatcgcgtgt acctgcatac
     4021 accggtagcc gcgaataacc tgactcgata attacatcca tcaactgctt gaaattcaat
     4081 ttaatatcca ctatcaccat gtccatgcgc gaggtcatca catcagctac ctgaatgttg
     4141 gcaaatttaa tgattccttc gagcatttcc ttgtcttctt cctgtttgtc gtcggagagg
     4201 tccaacgcgt gcgaaagctc atccatcgaa atcatattcg ggcttctctt gggcaaccgg
     4261 ctgtttacca gtgccttgga ttcggcaagg atgtccacaa atggtttgaa aaaattctga
     4321 agtgccagaa tgattccgga atttttaagc accgtttcgg tagaatattc cgaggaatat
     4381 accttcggca tgatttgtcc gaaggtaagc aaggtaacgc tcacaaacaa aacgggagca
     4441 ataaatttcc agaccggagt cagttcaaaa tcaaaaaccg aatagatgag tgtggtggaa
     4501 agaatgacga atgcgatgtt ggcaacacta ttccctatca tgatggtagc cagcagtttt
     4561 tgaggagaat tgagcagttt gatggttgtt tttgctgatt tctcttcgct ctcgtgcatt
     4621 tctgccagat tttttggcga aagggtgaaa taagctactt cagaagaact gaaaaaagca
     4681 gaaaatgcca gaaacagcat ggacagaacc agcagcacgt aatcgctcca ctgaggagct
     4741 gtgaaatata catcggtgaa aatattgagc aacatagggt tttattacaa aatcagagtt
     4801 tatttattca tgcaaatata caaaaaagtt ctgttttact ctccgaaaac gtaataagtt
     4861 tcaaattctg aattcattat gctgtgttct gtattttgtg ttctttgcta tttgttcttc
     4921 aatcctgaaa gttttttcgt aattttgcag caaaacattt ttttttatgc gtattgatat
     4981 tatttctgcc gtacccgaac tgctggatag tccgttgaat cattctatcc tgaagcgggc
     5041 caaagaaaag ggactggtgg agatttttgt tcacagcctg cgtgattata cggatgacaa
     5101 gcatcgccgg gtagatgatt atgcatttgg atttggcgcc ggcatggtgc ttcagattga
     5161 gccggtggat aatgcaattt cggcactgaa atcggcacga agctacgatg aaatcattta
     5221 tacctcgccc gacggcgaaa aatttaccca gaagctggct aaccggtatt cgctggccga
     5281 aaacctgatt attctctgcg gtcattacaa aggaatcgat caccgaattc gtgagcactt
     5341 gattaccagg gagataagcg tcggcgattt tgtgctcacc ggaggcgaac tgcctgctgc
     5401 catcatggcc gatgccatag tcaggcttat tccgggtgtg atcggcgatg aaacttcagc
     5461 actttcggat agtttccagg ataacctgct tgcaccgccc gtttacacgc gccccgctga
     5521 ttacaaaggc tggaaagtac ccgaaattct cctttcgggg catcaggcga gggtggagga
     5581 gtggatgcat cagcagtcgg tggagcgtac caggctctta cggcctgatt tactggagga
     5641 atgacacagt gaatgcgttt tttacatatc tggtggttgt aaatattgta tctgcagttc
     5701 tcttcactta cgataaagtg gcagcaatca gacgaaaacg gagaattccg gaacttgttc
     5761 ttcacttttc agaggtaaca ggaggtgtgt ttttcgtggt aatgctgatg tatcttatca
     5821 atcacaaaaa ccgaaaaccg gattattacc gcattactta cgttgttctg atagtctgga
     5881 cggtaattgc agggtggttt tttacataaa aaattaagaa ataataaaga atttgatgtc
     5941 gggaaaaaag caaagcattg aatccaggtt atttcacgga gtgaaagaat ttcgtgaaaa
     6001 cgatttcgag caaaaccggg aagtgttcaa aaatctggaa agtagtcagc atccgcatac
     6061 gctttttatc ggatgctccg attcgcgagt ggtacccaat ctgataacaa gtaccctgcc
     6121 gggcgaattg tttattatac ggaatatcgc caatctggtg ccttattaca aggaaaacca
     6181 cgacacctac gtagctactt catcggccat agaatatgca ttgcatgtgc tgaaggttga
     6241 aaatattatc gtttgcggtc actccaactg cggaggctgc agagctttgt attcagacag
     6301 gttgcagaat cagcttccgc ttacgcgaaa atggcttgaa cttgctgcgc cagtcaaaaa
     6361 actggtgaag gcaaaaattc tgaatgaaaa gttgaaacca tgccggcgcg atatgctaac
     6421 cgagcaactc aatgtggtgg agcagatcaa tcatttgctt acatttccgt atatcaaaga
     6481 gagatacgac aagggagaac tcaacatcat ggggtggtat tatgtgattg aaaaaggcga
     6541 agtatataac tattcgcatg ataatcaggt gtttgaaaga atcgagtagg tgaagtttag
     6601 cctgttctga tttaaattta tacttagttg caataaaata ataactacaa atggcctgga
     6661 tttttttaat tatcggaggc gttttcgaat cactgttttc cttcagtctg ggaaaaatat
     6721 ctttaacttc cggcagggaa atgtttctct ggatactggt ttttgttttt tcggtgagcc
     6781 tgtccatgta ttttctttac aaagcaatag attcgggcct gggtgtcggc gtttcttatg
     6841 ccgtctgggc agccatcggt gcagccggaa gtgtgttgat ggggattttt ctgcttgctg
     6901 aaccagtttc tttctggcgg atatttttcc tcactttgct cgttgtttcg gttgtaggtt
     6961 tgaatttaag ctctcatccg gcatcgtagc agaatattta caaaacaatt aatttattga
     7021 taatgatatg attaaagcag gagtaattgg tggtgcgggt tatacggccg gagaattgat
     7081 tcggattctc gttcatcatc cagaagtgaa aattgagttt gtaaacagca caagcaatgc
     7141 cggaaatcca attaccgctg ttcatggggg gctcattggc gaaaccggtc tgatttttac
     7201 cgacgaattg cctctcggcg aaacggatgt tttgtttctt tgttcggcgc atggcgacag
     7261 ccggaagttt ttagctgctc atgaagtgcc tgaaaatgta aggataatcg atttatcgac
     7321 cgattttcgc cacaaacgtg atgatcacga ttttgtgtac gggcttccgg aactcaacaa
     7381 aaatgcaatc atacacgcaa accgcgtagc caatccgggc tgttttgcca cgaccataca
     7441 actggcactt ctgccattgg cagcagccgg tttgctcacc gacgatattc acgtaaacgc
     7501 tattacgggc tccaccggtg cgggtgtgaa accctcatca acctcccatt tcagttggag
     7561 aaacaataat atttccgttt acaaagcctt tgaacatcag catctggatg aaatctttca
     7621 atccatccat cagcttcagc ccggttttgc caaagacctg aatttcatac cggtacgtgg
     7681 aaatttcgca cgcggaattt ttgctacaac ctatacaaaa agttcgctga gtgcagcaga
     7741 agccgaaaag atttaccgcg acttttacaa tgatgccgct tttacacacg tggtaagtga
     7801 aaatccggat atgaagcagg tggtcaatac caacaaagcc atagtgcatg ttgaaaaaca
     7861 cggagaatat ctcctgatta tttctgctgc tgataatttg ctgaaagggg catcgggaca
     7921 ggcggtgcaa aatatgaatc tgatgttcgg actggatgaa aaaacgggat taaacctcaa
     7981 atcaatcggt ttttgatgaa attgattaaa aaaagaaata ttttttgcaa acgtttgaga
     8041 aaaatttcgg cagaaaaaca ggaaaaaaca gggggtgtga ataaaatctc cgatatcttt
     8101 gcatcatgat tttttcatag tattagattt aaggttaaca aagattggtt gtcaggcgtg
     8161 gcaacctttc gcgtttttaa agagcaggta gctcttttat cacggctcta aatctcactc
     8221 agttccagcc accgcatggt tttttcgtca attaatgctg tcagttcacc gaatcgctct
     8281 gatgcagcca atatggcttc attatcgagc attccggatg aaagccgaac ctcaatctcg
     8341 gttttttcgg cttccagttg cggtatttcc ttttccagcg cttcaaattc ctgcttttct
     8401 ctgaaactca ttttgcgggg ttttccctca atcctgtttt cattgggagt gggagtgttt
     8461 tttcgctgat ttttttcact tgcaggtttt gaagtccctc cttcggaggg atttagggag
     8521 gcctctctgt attgcgtgta attgcccgga aaatcgcgca cgacggcatc gccttcgaac
     8581 acgaaaagat gatccaccac cttgtccatg aaaaaacggt cgtggcttac cacaataacg
     8641 caccccttga atgtgagcag ataatcttcc agtatgttga gtgtcacaat atccagatca
     8701 tttgttggct catccagcac cagaaaattc ggattctgca tcagcacggt gcaaagatga
     8761 agccgccttt tttcgcctcc gctgagtttg tacacgtaat tatgctgtgt ttccggcgaa
     8821 aaaagaaaat agttcaaaaa ttgcgagacg ttcatctttt tgccgttgcc caggtctact
     8881 tcttctgcaa tatctctcac tatatcaatc actttcatct gttcgtcgaa catcagccct
     8941 tcctggctgt aatacccgaa ctgaactgta tcaccaatat caacactgcc gctgtccggc
     9001 ttcaactggc ccagcagaat cttcagaaaa gtagttttac cggttccgtt tttccccaca
     9061 attcccagtt tttcaaatct gccgaaattg tagtaaaaat tatccagaat tttcaaatct
     9121 ccgtaagatt tgctgatgta ttttgcttca aaaattttgg aaccaaggta agatgccttc
     9181 actgccagtt ttacgttgtc gtttaccagt cgctgctttg cccgttcttc aatgtcgtaa
     9241 aaacggtcga tacgcccttt tgattttccc gtacgggctt gcggagtgct tcgaatccat
     9301 tccagttcgc ggcggtaaat attctgagct gaggtggttt cggcattgaa attgtcaatg
     9361 cgctcctgtc ttttctcgag atagtagctg taattgcctg aataactgaa taaattatga
     9421 ttttcaagct cgaggatgtt ggtacatacc cggtcgagaa aataacggtc gtgggtgacc
     9481 atcagcagcg ccatggacga tcgcctgaga aagtcttcga gccattccac catttccaga
     9541 tccaggtggt tggtcggttc gtccagaatg agcaattcgg gttcgctgat aagtacgttg
     9601 gccagtgcta tccgtttgag ctgacctcct gaaagttcgc caattttttg ttcaaagttg
     9661 gtgattttga gtttacccag tatttgcttg atgcgcgatt cgtaatccca ggctttcagc
     9721 atttccattt ctgcaagtac ttctgacaag gtatcgtttt caggatgaag cattgcgtgc
     9781 tcgtatcggg caatggtgtt catcagttgg ttgtccgatt gcaggcaggc gtcaattacc
     9841 gaaagttcgg aaggcaattc gggcgattgc tccaggtatc cgatccggag gtctttccgg
     9901 aatgagatat ggccactgtc gggggattcc cggtcggcta agatattcag aagggttgac
     9961 tttccggttc cgtttttggc aatcagggct acccgctgat tgtcggctat accgaatgaa
    10021 atgttttcaa atagtaattt gtctccgaat gatttggtta aattctctac ctggatatag
    10081 ttgaccatac aaaaaaaata ttttgtacaa aggtagttat tcgttgtgag aagacaagaa
    10141 aaccggtacc ttaactccgg aaaaaatgtt gactaaaaat ccagctcggg catattttta
    10201 tgctcttctt cctcaatccg tttgcggaaa tcgaactgtt cgtcggctct gtaaatttcg
    10261 cccagctgat cgagatacga aaagagtttt ggcaggagtt tccggttgaa agttatctgc
    10321 gaggcggtgc gcggcaggcg gaatccgaat gtttttctca gacgggagtt ttgccagagg
    10381 cagggaacat ttcgttcaat gtcttttgtt ttatctattt cttttgaatt gtttaaaatc
    10441 cagctccaga ccagttttcc gattctgtcg ttggtgagtg agcagttgta atttttccgt
    10501 acatagagaa taaattcatc gtttcccatg gttattgtgt ttttcatatt ctgaaagtat
    10561 taaaatgggt gttaagattt tatttttcaa ttccttacaa atatatgaaa ttgctttata
    10621 tatccaaaaa aaatccggtg attttttttc accggatttt tctgttttat ttcattctaa
    10681 aaaaaatcgt tagcatataa cctgtttttt gatgagctgg aagaagtcgt ttcctttgtc
    10741 atctacaagt ataaatgccg ggaaatcgac cacgtcgatt ttccagatag cttccatacc
    10801 gagttcggga tattccagca cttctacatt cttaatgctg tcctgcgcca
//
LOCUS       DAORKP010000073        10783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_715_length_10783_cov_9.321588, whole genome shotgun sequence.
ACCESSION   DAORKP010000073 DAORKP010000000
VERSION     DAORKP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_10783_cov_9.321588"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..440
                     /gene="rplL"
                     /locus_tag="PLN75_05520"
     CDS             63..440
                     /gene="rplL"
                     /locus_tag="PLN75_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRG03609.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKDEYGIEPAAAAVAVAGP
                     AAGAAAPVVEEKTSFDVILKAAGPNKLAVVKLVKELTGLGLKEAKDLVDAAPSAIKEG
                     VTKDEADALQKSLTEGGAEVELK"
     gene            554..4366
                     /gene="rpoB"
                     /locus_tag="PLN75_05525"
     CDS             554..4366
                     /gene="rpoB"
                     /locus_tag="PLN75_05525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRG03610.1"
                     /translation="MSSTTNNQRINFASIKNQMDYPDFLEVQLKSFHDFFQMDTAPER
                     RKTEGLYKVFNENFPIADTRNNFVLEFLDYFIDPPRYTIEECFERGLTYSVPLKAKLK
                     LYCTDPEHEDFDTVIQDVYLGPIPYMTPKGTFVINGAERVIVSQLHRSPGVFFGQSLH
                     ANGTKLYSARIIPFRGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFNLAEEIKVNKTNLKKVVGRKLAARVLKSWVEDFVDEDTGEVVTIERNEVIIDRETV
                     IDSDHIDMIIDSGAQNVLIHKDDANQSDYAIIYNTLQKDPSNSEKEAVLYIYRQLRNA
                     EPADDSSAREVINNLFFSEKRYDLGDVGRFRINRKLNLSIDDEIKVLTKEDIIEIIKY
                     LVELINSKAEVDDIDHLSNRRVRTVGEQLYNQFGVGLARMARTIRERMNVRDNEVFTP
                     VDLINSKSITSVINSFFGTNALSQFMDQQNPLAEITHKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCIYAKINDLGFISTPYRKVTDAKVDISNEG
                     IAYFTAEEEEDLVVAQGNAPLNDDGSFIRKTVKTRLGADFPVVTPSEVHLMDVSPSQI
                     ASIAAALIPFLEHDDANRALMGSNMMRQAVPLLRCEAPIVGTGIEGQLIRDSRTQIIA
                     EGEGIVEFVDATTIKVKYERTEEEEFVSFDTAVKTYELPKFMKTNQNTTIDLRPIVKK
                     GDKVKAGQILTEGYATEKGELAIGKNLKVAFMPWKGYNFEDAIVINEKVVREDVFTSI
                     HVVEQLLEVRETKRGIEEFTADIPNVSEEATRDLDENGIIRIGARIEPGDIIIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIAKRLFSKAQKNRKTKLAD
                     KAELPKLEEQFEKQAAKLKDTLIEKLLLIIEDKASAGVKDFLGTDIISRNSKFTAERL
                     SEIDFTTIQLSKWTTDSHKNELIRQLILNYLQKFKELDAQIKRQKFNITIGDELPNGI
                     VQMAKVYIAKKRKIRVGDKMAGRHGNKGIVSRIVRQEDMPFLEDGTPVDIVLNPLGVP
                     SRMNLGQIFETVLGWAGKEMGVRFATPIFDGAELSDLDEWCDKAGIPRYGKTYLYDGG
                     TGERFDQTATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEME
                     VWALEAFGASHILQEILTVKSDDVMGRARTYEAIVKGDPMPAAGIPESLNVLLHELRG
                     LALSVNLE"
     gene            4522..8829
                     /gene="rpoC"
                     /locus_tag="PLN75_05530"
     CDS             4522..8829
                     /gene="rpoC"
                     /locus_tag="PLN75_05530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRG03611.1"
                     /translation="MAFKLDNKSKSSFSKISIGLASPEEVLKLSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPTKDFECHCGKYKRIRYRGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGMPTKKLDAIIYYEKYVIIQAGILENNENITYGELL
                     TEEEYLNLLETLPRENQMLEDTDPNKFIAKMGGDAIYDLLNRLDLDVLSYDLRNKANT
                     DTSQQRKTEALKRLQIVESFRASKNRNKPEWMVIKVVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQESVDSLFDNSRKSSAVKTDAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FVIRKLIERGIVKTVKSAKKVIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKMIEGKAIQLHPLSCTAFNADFDGDQMAVHLPLGNEAILEAQMLMLASHNILNPAN
                     GAPITVPSQDMVLGLYYITKPRPGARGEGLIFYSPEEAEIAYNEGRVDLHAIIKVRTK
                     DLDENNQLTTQLIETTIGRILFNKNVPAEMGYINELLSKKSLRDIIGRVIETCGVART
                     AQFLDDIKDMGYYMAFRGGLSFNLSDVIIPVEKEKLVKEGNDEVEEIMNNYNMGFITP
                     RERYNQIIDTWTHVNTKLSNVLIKQLADDRQGFNSMYMMLDSGARGSKEQIRQLAGMR
                     GLMAKPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVSQDVIINEEDCGTLRGLIATEVKNNEDVISSLYERILGRVSVHDIYHPLTG
                     ELIVESGAQITEQKAKIIESSPIERVEIRSVLTCESKKGVCAKCYGRNLATGRLVQMG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKIALKYDGRLEFDELRTVDAKD
                     EEGKEYSVVVSRLTEMRVIDTNTGIVLTTQNVPYGSKLYAKNGEIGKKGQLICTWDPF
                     NAVIIAENEGKIEFEDVIENVTFKNEVDDTTGLREKIIIESKDKTKVPSANILDPNGN
                     IIRTYNLPVGAHLILDEKDEIKAGQMLVKIPRAVGKAGDITGGLPRVTELFEARNPSN
                     PAVVAEIDGEINFGKIKRGNREVSVTSKTGEVKKYLIPLSKQILVQENDFVRAGTPLS
                     DGATTPADILAIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIIDQ
                     GDTRFLEKQIVDKHEFMEENDRIWGKKVIIDAGDSDSLRPGQIITARKLRDENSVLKR
                     KDLKVVQTRDAVAATSSQVLQGITRAALQTNSFMSAASFQETTKVLNDAAINGKVDKL
                     DGLKENVICGHLIPAGTGLREYEKIIVGSRDEYEKMMDARRNVTDIAQEHDMDDALN"
     gene            9121..10656
                     /locus_tag="PLN75_05535"
     CDS             9121..10656
                     /locus_tag="PLN75_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRG03612.1"
                     /translation="MKIKISLFISLFFSICLNAQESSLWRMIFEDEFSASTFDTRYWS
                     YCTRANPDWAKYLTSSSETVNTADGLLKVKLIKNTNTGTDNVPYLSGGIQSRKKFNFT
                     YGKVEVRAKFSNGQGSWPAIWMMPEDGSAGWPACGEIDIMEHVNSEDRIYQTIHSDFV
                     NTQGNRNPSPTQTTSIDKNAFNVYGVEWHPDRLDFTLNGNVTFSYPRINTTKTGQWPF
                     DKPFYIILNMAGGGSWTGPISEAALPFEMQVDWVKVYERNPEGPYIIPAWKSAKTQND
                     PYWANTFVQQITTSGAQNDINFQAQKRHETYYYQHPDTLLVTEGSNLDFNLKAFSLGN
                     YTESKVLQDLRYTCNYVFADFDGDKRFEMSLPRIGKVPPSNGVGGNMDVMNITRSVGI
                     PYNKDKNGRIRIVYDNAWNERYSPEIYVYEGVVYDFPIRITAQNTGIKDKIVQPTVKR
                     VGDVLISDYIEGNFKVEVFNPIGQQLINKRFTDSSLNEQLPKGLMIIRITDSSGRNFR
                     YKM"
BASE COUNT         3469 a         2116 c         2367 g         2831 t
ORIGIN      
        1 gaaaacactc ggtgaaagac aataaataaa ttattaacaa aaaaatatta aaataaaata
       61 aaatggcaga tttaaaagct tttgcagaac aattggttaa cttaacagta aaagaagtga
      121 atgagttagc tcaaattttg aaagacgaat atggtatcga accagcagct gcagcagttg
      181 cagtagcagg tcccgcagca ggtgctgcag caccggtggt tgaagaaaaa acatctttcg
      241 atgtaattct gaaagctgca ggtcctaaca aattagctgt agttaaatta gtaaaagaat
      301 taacaggtct tggcttgaaa gaagctaaag atttagttga cgctgctcct tctgcaatca
      361 aagaaggtgt aactaaagac gaagctgatg ctcttcaaaa atcacttact gaaggcggag
      421 ctgaagttga acttaaataa aaaaacctga ttatcaggtg tttggtttag attcccccga
      481 ttcgggggat tctaaacctt tttctgtcta tactaaatat tgagttctcc gcactaaaaa
      541 accatctttg acaatgtctt caactactaa taatcaaaga ataaattttg cttcgattaa
      601 aaatcaaatg gattatccgg attttctcga agtacaatta aaatctttcc acgacttttt
      661 tcaaatggac acggcgcctg aaagaaggaa aactgaggga ctttacaaag ttttcaacga
      721 aaacttcccg attgccgaca cccgcaacaa ttttgtactt gaatttctcg attacttcat
      781 agatccaccc cgatatacca ttgaggaatg tttcgaacgt ggattaacct atagtgttcc
      841 attaaaagcc aaattaaaac tttattgtac cgatcccgaa cacgaagatt ttgataccgt
      901 gattcaggat gtatatctgg gtccaattcc atatatgact cccaaaggca cattcgttat
      961 caacggagcc gaacgggtga tcgtatcaca attacaccgt tcgcccggtg tgttcttcgg
     1021 acaaagtctg cacgccaatg gtacaaaact atattctgca cgtattattc ccttccgtgg
     1081 ttcatggatt gagtttgcta ccgacatcaa caatgtgatg tatgcataca tcgaccgtaa
     1141 aaagaaatta cccgtaacaa cactgttgcg tgctatcggc tttgaaagtg acaaagacat
     1201 tttggaaata ttcaacctgg ctgaagaaat caaagtcaat aaaaccaatc tgaaaaaagt
     1261 agtcggacgt aaacttgcag cacgtgtgtt gaaatcgtgg gtagaggatt ttgtagatga
     1321 agataccggc gaagtggtta ctatcgaacg taatgaagta atcattgacc gtgaaacagt
     1381 aattgacagt gatcatatcg atatgattat tgattcggga gctcagaatg tactcataca
     1441 caaggacgat gccaaccaga gcgactatgc aatcatttac aatacattgc agaaagaccc
     1501 gagcaactct gagaaagagg ccgtacttta tatttatcgt cagttacgta atgccgaacc
     1561 ggcagatgat tcttcggcgc gtgaagtaat caacaacctc ttcttctctg aaaaaagata
     1621 tgacctgggc gatgtaggcc ggttccgtat caatagaaaa ctgaatcttt cgattgatga
     1681 tgagattaag gtactgacca aagaagatat tatcgaaatt atcaaatacc tcgttgaact
     1741 gataaattcc aaagccgaag tagacgatat cgaccacctt agcaaccgta gggtacgtac
     1801 agtgggcgaa caactctata atcagttcgg agtaggactc gcgcgtatgg cccgcactat
     1861 ccgcgagaga atgaatgtgc gtgataatga ggtatttact ccggttgacc tgataaattc
     1921 aaaaagcatt acctcggtaa tcaattcatt tttcggaacg aatgcacttt cgcagtttat
     1981 ggatcagcag aatccactgg cagaaattac tcacaaacgt cgtttgtcgg ctttaggccc
     2041 aggcgggtta tcgcgtgaaa gagccggctt tgaagtgcgc gacgttcact acacgcatta
     2101 cggacgtctc tgcccgattg aaactccgga aggccctaac atcggactta tttcttcgtt
     2161 gtgtatatat gccaaaatca atgatttagg atttatttcc acaccctacc gtaaagtaac
     2221 tgatgcaaaa gtagatattt caaacgaagg aatagcttat tttacagccg aagaagaaga
     2281 agatttagtg gtagcacagg gtaatgcacc gctcaacgac gatggcagtt ttatccgtaa
     2341 aactgtaaaa acccgtttag gagcagattt ccccgttgta acaccatctg aagttcattt
     2401 aatggacgtt tcgccctcac aaattgcttc cattgcagct gcattaattc cattcctgga
     2461 acacgatgat gctaaccgtg cactgatggg ctcaaacatg atgcgccagg cagttccgct
     2521 gctccgatgc gaagctccga ttgtaggaac cggaatagaa ggacaactca tccgcgactc
     2581 ccgtacacag attattgcag aaggcgaagg aattgttgaa tttgttgatg ccactacaat
     2641 aaaagtgaaa tacgaacgca ctgaagaaga ggaatttgta agttttgaca ccgctgtcaa
     2701 aacatatgaa ttacctaaat tcatgaaaac caatcagaat acaaccattg accttcgtcc
     2761 gatagtaaaa aaaggcgaca aggtaaaagc aggtcagatt ctgacagaag gttatgctac
     2821 cgaaaaaggt gaactggcaa tcgggaaaaa cctgaaagtg gcattcatgc cctggaaagg
     2881 gtataatttt gaagatgcca ttgttattaa cgagaaagtg gtacgcgaag atgtgtttac
     2941 ttccattcat gtagttgaac aattgctgga ggtacgtgaa accaaacggg gtatcgaaga
     3001 gtttacggct gatattccca acgtaagcga agaagcaaca cgggatctgg acgaaaacgg
     3061 tattatccgc atcggtgcac gcattgaacc cggcgatatt atcatcggta aaattacacc
     3121 gaaaggggaa tcagatcctt caccggaaga aaaactcctt cgggctatct ttggtgataa
     3181 agcaggtgat gtgaaagatg cctcgttgaa agccacacct tctttgtcgg gagttgttat
     3241 tgctaaacga ctcttctcga aagcacagaa aaaccgaaaa acaaaactgg ccgacaaagc
     3301 cgaacttccg aaactggaag aacaattcga aaaacaggca gccaaactca aagataccct
     3361 gattgaaaaa cttctgttga ttattgaaga taaagcttct gcgggtgtaa aagattttct
     3421 gggtaccgat attatttcac gaaacagcaa attcacggct gagcgactaa gcgaaattga
     3481 ttttactact attcagcttt ccaaatggac tactgatagt cataaaaacg agctgatccg
     3541 tcagttgatt ctgaattacc tgcagaaatt caaagaactc gacgctcaga taaaacgcca
     3601 gaaattcaat ataacaatag gtgatgaact tcctaacgga attgtacaga tggcaaaagt
     3661 ttatattgcc aaaaaacgca aaattcgcgt tggggacaaa atggccggtc gccacggtaa
     3721 caaaggtata gtgtcgcgta ttgtgcgtca ggaagatatg ccattcctcg aagacggaac
     3781 tccggttgat atagtactga atccgctcgg tgtaccatca cgtatgaacc tcggacagat
     3841 tttcgaaacc gtactgggat gggcaggaaa agaaatggga gtaagatttg caactcctat
     3901 atttgacggg gccgaattaa gcgaccttga tgaatggtgc gacaaggcag gaattccacg
     3961 ctatggtaaa acttatcttt acgacggagg aaccggcgaa cgtttcgacc agacagccac
     4021 cgtgggtgtg atttatatgc tcaaactcgg tcacatggtt gacgataaaa tgcatgcacg
     4081 ctccatcgga ccatactcac tcattacaca gcagccactt ggaggtaaag ctcagtttgg
     4141 tggtcagcgt ttcggggaga tggaagtctg ggcactcgaa gctttcggtg catcgcatat
     4201 tttgcaggaa atccttaccg tgaagtccga cgacgtgatg ggaagagccc gtacatacga
     4261 agctatcgta aaaggcgatc cgatgccggc tgccggaatt ccggaatctc tcaatgtgtt
     4321 gttacacgaa ctgagaggtt tggcactaag tgtcaatctg gagtaaaaga tccgccccat
     4381 gacctcctga agggagaata attggtgggg aaatcaatat ttcctaacgt aatgacgttg
     4441 aaataaagct tcatatattt ttaaaaattt aattaacgct ctaaattctc ctttcagggg
     4501 gtaggggcat attatacgaa catggctttt aaattagata ataaatcaaa aagtagtttt
     4561 agtaaaatct ccatcggatt agcttcgccc gaagaagtgt tgaaactttc aagcggagag
     4621 gttttgaaac ctgaaactat caattatcgt acttacaaac ccgaacgcga cggattattt
     4681 tgcgaaagga ttttcggtcc gacaaaagac tttgaatgtc attgcggcaa atataaacga
     4741 atccgttatc gtggtatcgt ttgcgaccgg tgcggtgtgg aagtcaccga aaagaaagta
     4801 cgccgtgaga gaatgggaca tattcaactg gttgtgcctg tagcacatat ctggtatttc
     4861 cgctcgttgc caaacaaaat cggttatctc ctcggaatgc caactaaaaa actggatgca
     4921 atcatttatt acgaaaaata tgtgataatc caggccggta tacttgaaaa taacgaaaat
     4981 atcacttacg gtgaactcct tactgaagaa gaatacctga atctgctgga aacacttccg
     5041 cgtgaaaatc agatgcttga ggataccgat ccgaacaaat tcattgcaaa aatgggtggc
     5101 gatgccattt acgatttgct caatcgcctc gatctggacg tgctttcata cgatctgcgt
     5161 aacaaagcaa acaccgatac ctcacagcaa cgcaaaacag aagcattgaa acgtcttcag
     5221 attgtagagt ccttccgtgc atccaaaaac cgaaacaaac cggaatggat ggtcatcaaa
     5281 gtagtaccgg taattccacc ggaattacgt ccgctggttc cgctggatgg aggccgcttt
     5341 gctacttctg acctgaatga cctgtaccgt agggtgatta tacgcaataa ccgattaaaa
     5401 agattgattg aaattaaggc tcctgaagtt attctgagaa atgaaaagcg tatgcttcag
     5461 gaatcggtgg attcactgtt tgataattca cgcaaatcaa gtgcggttaa aaccgatgca
     5521 aaccgtccgc tgaaatcact ttccgacagt ttgaaaggaa aacagggccg gttccgccag
     5581 aacctgcttg gtaaacgtgt ggattattct gcccgttcgg ttattgtggt aggcccggaa
     5641 ctgaaaatgc atgaatgcgg attgccaaaa gatatggctg ccgagcttta caaaccgttt
     5701 gttattcgaa aacttatcga gagaggaata gtaaaaacgg taaaatcggc caaaaaagta
     5761 attgaccgaa aagatccggt gatttgggat attttagaat acgtgatgaa aggacatccc
     5821 gtactgctaa accgtgcccc tacacttcac cggttgggta tccaggcatt ccagccgaaa
     5881 atgattgaag gaaaagccat acaattgcac ccgctgtcat gtactgcgtt caatgccgac
     5941 ttcgatggcg accagatggc tgtacacctt ccactcggaa acgaagcaat actcgaagca
     6001 caaatgttga tgcttgcatc tcacaatatc cttaatcctg ccaatggagc tcctatcact
     6061 gtaccttcgc aggacatggt gcttggactg tactacatca ccaagccacg tccgggagcc
     6121 agaggtgaag gattgatatt ctattcgccc gaagaagctg aaattgctta taacgaaggt
     6181 agagtagatt tacatgctat cattaaggtg agaaccaaag acctggatga aaataatcag
     6241 ctaaccacac aactgattga aacaaccatc ggaagaattt tattcaataa aaacgttccg
     6301 gctgaaatgg gttatatcaa tgaattgctt tccaaaaagt cattgcgcga tataatcgga
     6361 cgtgtaatcg aaacctgcgg tgtggcccgt acagctcaat tcctcgacga tatcaaggat
     6421 atgggttact acatggcctt caggggtggt ttgtcgttca atctgagtga cgttatcatt
     6481 ccggtagaaa aagagaaact cgttaaagaa ggtaacgacg aagtggaaga aatcatgaac
     6541 aattataaca tgggattcat cactccgcgc gaacgttata atcagattat tgatacatgg
     6601 actcacgtaa atacaaaatt gtctaatgta ttaattaaac aacttgctga tgaccgtcag
     6661 ggatttaact ccatgtacat gatgcttgac tcaggtgccc gtggatcaaa agaacagata
     6721 cgtcagctcg caggtatgcg tggtttgatg gcaaaacctc aaaaatcggg cgctgaaggc
     6781 ggacagatta ttgaaaatcc gattttgtcg aactttaagg agggactttc ggtactggag
     6841 tattttattt ctactcacgg tgcccgtaaa ggtttggccg atactgccct caaaactgct
     6901 gatgccggtt atttgacccg tcgtttggtc gatgtttcgc aagacgtgat tatcaacgaa
     6961 gaagactgcg gaacattacg aggactgatt gcaactgaag tgaaaaacaa cgaggatgtg
     7021 atttcatctc tttatgagcg tattctggga cgtgtatcag ttcacgatat ttatcatccg
     7081 ctgaccggag aacttatagt ggaatccggt gcacaaatta ccgaacagaa agcaaaaata
     7141 attgaaagtt ctccgattga acgtgttgag attcgttcgg tactgacctg tgaatcgaaa
     7201 aaaggagtat gtgccaaatg ttacggacga aacctggcta ccggtcgact ggtacaaatg
     7261 ggtgaggcgg ttggtgtcat agctgctcaa tcaatcggcg aacctggaac tcagttgaca
     7321 cttcgtacat tccacgtcgg tggtattgct tccaacattg cagccgaatc aaaaattgct
     7381 ttgaaatatg atggtcgtct tgaatttgat gaacttcgta cagtagatgc caaagatgaa
     7441 gaaggaaaag aatacagcgt agtggtaagc cgtctgaccg aaatgcgtgt cattgacacc
     7501 aataccggaa tcgtactcac aactcagaac gttccttacg gatcgaaact ttatgcgaaa
     7561 aacggtgaaa ttggcaagaa aggtcagttg atctgtacgt gggacccttt taatgctgta
     7621 attattgcag aaaatgaagg taagattgaa tttgaagatg taatagaaaa tgttacattc
     7681 aaaaatgagg tagatgacac caccggtctg cgtgaaaaga ttattattga atccaaggat
     7741 aagactaaag ttccaagtgc caatattctc gatccgaatg gaaacattat acgtacatac
     7801 aacctgccgg tgggagctca ccttattctg gatgaaaagg atgaaatcaa agcaggacag
     7861 atgcttgtta agatacctcg tgcggtgggt aaagccggcg atatcacggg aggtctgcca
     7921 cgtgtaactg aattgttcga agcacgtaat ccatccaatc cggcggttgt agccgaaatt
     7981 gatggtgaaa taaacttcgg aaaaatcaaa cgtggaaatc gtgaagtttc tgtaacttct
     8041 aaaacaggtg aagtgaagaa gtatttgatc ccactttcga agcagattct ggttcaggaa
     8101 aatgattttg ttcgtgcagg cactccgctt tccgacggtg caactacacc ggctgatatt
     8161 ctggccatca aaggtcctac cgcagttcag gattacattg tcaacgaagt gcaggatgtg
     8221 taccgtctgc aaggtgtgaa aattaacgat aaacactttg aagtgatagt tcgtcagatg
     8281 atgcgtaagg tggagattat tgatcagggc gatacccgct tcctggaaaa acaaattgtt
     8341 gacaaacatg agtttatgga agaaaacgac agaatctggg gtaaaaaggt aatcatcgac
     8401 gccggtgatt cggatagcct cagacccgga cagataatca cagcccgcaa gcttagggat
     8461 gaaaacagtg tactgaaacg taaagactta aaagtggttc aaacacgcga tgctgttgct
     8521 gctacttcaa gccaggtgct tcagggaatt acccgtgcag cgttgcaaac caacagtttc
     8581 atgtctgcag cttcattcca ggaaactact aaagtactga atgatgcagc catcaacgga
     8641 aaagttgata aactggatgg attgaaagag aacgtaattt gcggtcattt gattcctgcc
     8701 ggaaccgggt tgcgtgagta tgaaaaaatc attgtgggct cacgggatga gtacgaaaaa
     8761 atgatggatg cacgacgtaa cgtaaccgat atcgctcagg aacacgatat ggatgatgca
     8821 ctaaattaat ttacttactc aactcttata taaaagaatg acaaaatctg caaatgatta
     8881 tgtcattctt ttttttactt tcttgtgcca ttttgagtgt tgtatcaatt taaaatatta
     8941 tcttcgtaca accttaaatt agattgtttg attaagtaga aaaactgatt ttttttcatt
     9001 ggatgatttt tttttatgaa taattttatt acaatcaggt aaatattttc ttcataaaaa
     9061 tgtattcaat aatcaaaaaa aggatttgta aataatcgac tttcataaat aataatacaa
     9121 atgaaaataa aaatctcact ttttatatcc ttgttttttt caatctgcct gaatgctcag
     9181 gaaagttctc tttggagaat gattttcgaa gatgaatttt cagcatctac tttcgatacc
     9241 agatactggt catactgtac acgcgccaat ccggactggg ccaaatacct cacttcgtcg
     9301 tcagaaacgg tgaataccgc tgatggtttg ctgaaagtaa aactcatcaa gaataccaat
     9361 accggtaccg ataatgtgcc gtatctaagc ggtggaatac aatcgcggaa aaaatttaac
     9421 tttacgtacg gcaaagtgga agtgcgggct aaattttcca acggtcaggg ctcatggccc
     9481 gctatatgga tgatgcccga agatggcagt gccggttggc cagcttgcgg cgaaatagac
     9541 atcatggagc atgtcaactc tgaagaccgc atttatcaga ccatccattc cgatttcgtc
     9601 aacacacagg gcaaccgtaa tccttcgccc acacaaacaa cttcaatcga caaaaatgca
     9661 ttcaatgtgt atggtgtgga atggcatccc gatcgtctcg actttacact gaatggtaat
     9721 gtaacatttt cctatccaag aataaataca acgaaaacag gtcagtggcc attcgacaaa
     9781 ccgttttata taattttgaa tatggcaggt ggtggaagct ggacaggtcc tatcagcgaa
     9841 gcagcattgc cgttcgaaat gcaggtggac tgggtgaagg tgtatgagag aaatccggaa
     9901 ggaccatata ttattccggc ctggaaatcg gcaaaaactc aaaatgaccc atattgggcg
     9961 aacacctttg ttcaacagat tacaacctcg ggtgcacaga atgatatcaa ttttcaggca
    10021 caaaaaaggc atgaaactta ctattatcaa catcccgata cactactggt aacagaaggc
    10081 agcaatctgg atttcaatct gaaggctttt tcgttgggaa attacacaga aagcaaagtc
    10141 cttcaggatt taagatatac atgcaactat gtttttgctg attttgacgg agataaacgt
    10201 ttcgaaatgt cgttaccacg aattggaaaa gtacctcctt ctaatggtgt tggtgggaat
    10261 atggatgtaa tgaatattac acgttctgtc gggattccat ataataaaga taaaaacgga
    10321 aggataagaa ttgtttatga taatgcatgg aacgaaagat attcaccgga gatatatgtt
    10381 tatgaaggag tggtatacga ttttccaata cggattacag cacaaaatac cggaattaag
    10441 gacaaaattg ttcaaccaac tgttaaaagg gtaggagatg ttttaatctc agattatatt
    10501 gagggtaact tcaaagttga agttttcaat cccataggac aacagctgat aaataaaaga
    10561 ttcactgact ccagcttaaa tgaacaatta cctaagggtt tgatgataat cagaattacc
    10621 gattcgtccg gtcgaaattt cagatacaag atgtaatttt aattgttttc caatcctatt
    10681 cgtaaaccaa tccggatttt gaaaggttat aattttattg attccgaaat tcttcagaat
    10741 catttttttt tgcacaacca gtaaatctga gtttttaaaa aga
//
LOCUS       DAORKP010000074        10654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_735_length_10654_cov_10.329182, whole genome shotgun sequence.
ACCESSION   DAORKP010000074 DAORKP010000000
VERSION     DAORKP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_10654_cov_10.329182"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1967)
                     /locus_tag="PLN75_05540"
     CDS             complement(207..1967)
                     /locus_tag="PLN75_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRG03613.1"
                     /translation="MFYACANRGQGPQGGAKDEVPPRFVKSTPREKSVNVKDGKIEIE
                     FDENVNLKDISKNVIISPPQRINPEITSYGRKVNVRFDDTLQSNTTYSIDFGNAIVDN
                     NEGNILKNYVFSFATGNEIDTMQIAGTLLNAEDLNPLQGITVGIHDDLSDSAFLKKPF
                     LRIARTDEQGRFTVSNVRNGIYRVFALNDLNRDYFLQKGEALAFDEKSFQTGMENITR
                     PDTVWKDSITVDTIRFVPAIRYLPDDVVLRYFKDDYKRQYLAKSERPEPHLIKMFFNT
                     SAEKLPEIRPLNVNWDKSVLLQKNQRLDSLTYWLTDSALIKTDTITLEVKYLKTDSLF
                     KLQPQTDTIKFVMKKPAKKPSVTGKKKEFLDIQTNLSPQFDVYKPVMLTFQVPVKSYE
                     IEKIHLSQLVDTVLTPIQFVLEKKDSIGMNFQINHKWIPETTYQLLIDSAAFTSVYNL
                     HSDNLKNELKIKSLDQYSGLKLLLANYDSTAILQVVNKDDKLIRTAPVLKGGTKVEYL
                     EPGDYYLRMFLDRNGNGKWDPGNYFEKRQPESVYYYEKKLTLIKNWEFEETWDHTALP
                     LLKQKPQELKKSDQQKPNNQ"
     gene            complement(2073..3356)
                     /locus_tag="PLN75_05545"
     CDS             complement(2073..3356)
                     /locus_tag="PLN75_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRG03614.1"
                     /translation="MKESILHYVWQFRLFRMNNLKTTDGKMVEIIHPGLPNTDAGPDF
                     FNAKIKIDDTLWAGNIEIHNQSSDWAKHRHTADKAYDNVILHVVNKADKAVFRTNGDP
                     LPQLELEVPEHIRMNYNELLSGKKWIPCGDKITQVPLILINDWKSALLIERMEQKTEM
                     IETLLTQTNNHWEEAFYVSLARSFGFGTNSQAFERLARSLPMSILAKHKDDIFQLEAL
                     LFGQAGLLEQAENDEYHAKLKKEYRFLQTKYHLKPIESSEWKLLRLRPDNFPHVRLAQ
                     FAALTHRSSKLFSKTIAAADIMQLRALFDCVVSDYWKKHYLFGKQSAESAKRLGRKSI
                     DILLINTVVPFLFAHSKKKNAECETALKLLELIPSEKNAVVRRWVELGVSSASAFDSQ
                     ALLHLKKKYCDDKKCLRCRIGHKVLSKSNNQVQLT"
     gene            complement(3502..4104)
                     /locus_tag="PLN75_05550"
     CDS             complement(3502..4104)
                     /locus_tag="PLN75_05550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRG03615.1"
                     /translation="MRQVPEIIKALWRTPVWRIKSAEKVIYLTFDDGPNPQVTPKVLD
                     ILDQWNVKATFFCVGENVMKFPGIFQEVKKRGHSVGNHTYNHLRGHKTSVREYAENVW
                     KAHELIQSSLFRPPHGRITFRQVNKLKKDFEIIMWDFITYDFDRRVKPAEILNEIKKR
                     SRSGSVVVFHDSEKAHSNVLATLPDALKFWKENGYEVKAI"
     gene            complement(4138..7362)
                     /locus_tag="PLN75_05555"
     CDS             complement(4138..7362)
                     /locus_tag="PLN75_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRG03616.1"
                     /translation="MKNFRFLNTIFGWLAFAVAAVTYLMTIEPTASFWDCGEFISAAY
                     KLDVGHPPGAPFFMLTAKFFSLFTSDPSKVAMMVNAMSALASAFTILFLFWTITHLAQ
                     KVIGKSEEEYTTANKIIVIGAGLVGALAYTFSDTFWFSAVEGEVYAFSSFFTAVVFWL
                     ILKWERNADNPGSDRWLILIAYLMGLSIGVHLLNLLAIPAIVLVYYYKKYTPSTRGIL
                     LAMAASVIILAFTLYGLIPGTIMWTTKFELLFVNVLGASYNTGLFVYIFMVIAALVWA
                     IYETHTGKNYTRMAVSFILAVTITGVPFLFENIILGIFIILAMIGFFAYKKKSVKPRW
                     LNTSLVMIAVIMIGYSSYAVIMIRSAAQPTMDQNSPDNVFSLQYYLNRQQYGDRPLLY
                     GGVYNAPMKLKVEGNTCTYDADKTPLYAPKPKRDASEKDEYVVAGYKFDYKKNEKFMM
                     LFPRMYSGDPNHVKAYKMWGNVVGKKVNYDFCGEQRSDIKPTFGENMRFFFDYQVNFM
                     YWRYFMWNFSGRQNDMQGYGEIEHGNWITGIPFIDNMLVGNQKLLPSELKNNKGRNVY
                     FMLPLLLGLLGIFFLLNAGKQGNHVFWAIMLLFFLTGLAIVVYLNQTPYQPRERDYAY
                     AGSFYAFSIWIGLGVMGLSKLLDKILPKNVSAAIAAIACLGIPVLMAAQNWDDHDRSG
                     RTTARDFGQNYLNSTEKNAILFSNGDNDTFPLWYNQEVEGVRTDVRVCNLSYLPTDWY
                     IDQMKRQAYESAPLPISWQKKDYISGRNDLMSVQNLTDGKPFNMKDAFEIIKSDEAEF
                     KIDGDGFFPSPKLSLPVDAAEVVKTGSVPAKDISKIVPEMIFDLPKSVFKGEAMVMEM
                     IKENNWKRPMYVCVTVGEEFYPQSLSKYLSRTGLVYQILPVGQQDSTRVNVNTDKMYD
                     NMMNKFRFGGVDNPKIYLDDQVLRMCKTHRIQFMYLAEALIEEGDTVRAKKVLEYANK
                     VIPAATVPHDYSSNMMALYYYQLGMKSEADAILDSVAGNSVEYLNWYASLKPSQQSNA
                     TSSIGHEMAVMNQVLQISSQAGSKTLLDKYMKQFEMFANKFNMR"
     gene            complement(7560..8219)
                     /locus_tag="PLN75_05560"
     CDS             complement(7560..8219)
                     /locus_tag="PLN75_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03617.1"
                     /translation="MKKLIFILSALFVFAACGQKQTGESKTDKDAAKEVAAQAKKGKK
                     YPIEKGIVYQKSNVMGIESNPVIYFDKWGEWEATETTTTMEMLGQKMSSTNITIVKGN
                     DNWDIDMDKKTGFHFNRAAVINQLGVDLETLTEETMGKMKLEKIGQEDYLGFPCVKYR
                     MKNDEMKMDVEYLASGNLMMKMKGKVMEIPTSIEVTKIEAVTPPAEKFEIPAGVTITE
                     Q"
     gene            complement(8357..9655)
                     /locus_tag="PLN75_05565"
     CDS             complement(8357..9655)
                     /locus_tag="PLN75_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRG03618.1"
                     /translation="MKRIVFFLCICFLFGLQMSAQGWLERAGQRVKDRVVEKVENRID
                     QKTDEATDKVLDNAEGSVKKSGKKNQKGQKDAEEAAESDENQDEDAVTNSSNKQKLES
                     VTAYDFVPGDKILFFEDFSQDALGDFPALWTTNGSGEVKTVNIAPGNWFHMNGENAVY
                     CYTKNISFPDNFIVEFDIIPDAEYYDGIRFTIYQDNPEDPQELNDDLYPGKGGLHIVP
                     GKEGWSTVGYTDSPDDRWLTGEASKNPVVTEKVNHVIIWIQKRRVRIYHQGAKVLDMP
                     TNIYPGIAFNKIRFSGWDVRSWPMVTNLKITTASPDTRSKLITEGKLVTYGITFDVNK
                     ADVKPESYGTLKSIADVLSENPTVKVKIIGHTDSDGDAAKNLDLSRRRAESVKNELSK
                     NFGIDATRMITEGAGETKPVAANDTPENKAKNRRVEFVKQ"
     gene            complement(9745..10326)
                     /locus_tag="PLN75_05570"
     CDS             complement(9745..10326)
                     /locus_tag="PLN75_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRG03619.1"
                     /translation="MNLINRAKSILLSPKTEWAVIEPEQTDVKTLTLNYLLPLALIPA
                     IAGFIGFGLIGQNVLGMHIGSAGYGLRYAIVSLVSTIGGAFLTAYIIDLLAPSFGSEK
                     NFLQSFKLVVYSYTPMMVAGVFLIIPALGILATLAGLYGLYLLYIGLQPMKKTPDDKK
                     TTYFVVSLIVVIIGSVVLSAILSSIFITSGIHY"
BASE COUNT         2802 a         2287 c         2194 g         3371 t
ORIGIN      
        1 cgctctgcca actgagctat tgccgcaatt ctatttacaa tttacaattg ctatacaact
       61 taacacgacc tctccgattg gaatcggagt gctctgccaa ctgagctatt gccgcaattc
      121 tatttacaat ttatctatat acaatttaca atttacaaaa tcagaaatgc tcagcaacaa
      181 tcttcctgat gcaaaaataa cacttcttac tgattatttg gcttttgctg atccgatttt
      241 ttcagttcct gtggcttttg ctttaacaag ggtaaagcag tgtgatccca ggtctcttca
      301 aactcccagt tttttatcaa tgtgagtttc ttttcgtaat aataaaccga ttcgggttga
      361 cgtttttcaa aatagtttcc cggatcccat tttccgtttc cattccggtc cagaaacatc
      421 ctcaggtaat aatcacccgg ctccaggtat tccactttcg tgccgccttt caatacagga
      481 gctgttcgta tcagcttgtc gtctttattt accacctgta aaatggctgt tgaatcatag
      541 ttggccaaaa gaagtttgag tccggaatac tgatccagcg atttgatttt cagttcgttt
      601 ttcaaattat cgctatgaag attatatacc gaggtgaatg ctgcagaatc aatgagcagc
      661 tgatacgtgg tttcgggtat ccatttgtgg ttaatctgaa aattcatgcc aatggagtct
      721 ttcttttcaa gcacaaactg aatgggagtc agcaccgtat ctaccaattg actgaggtga
      781 atcttttcaa tttcatatga tttaaccgga acctgaaaag tcaacatcac cggtttatac
      841 acatcaaatt gtggagaaag attggtctgt atatccagaa attccttttt tttgccggta
      901 acagatggtt tcttagccgg tttcttcatc acgaatttga tggtatcggt ttgtggctga
      961 agtttgaaca acgaatccgt cttcaggtat ttcacttcga gggtgattgt atctgttttg
     1021 ataagcgcag aatcggtaag ccagtatgtg agcgaatcca gccgttgatt tttctgtagg
     1081 agcacgcttt tgtcccaatt cacattcaga ggtctgattt cgggtagctt ttcagcagac
     1141 gtattaaaga acattttgat taaatgcggt tcgggccttt ccgattttgc cagatactgg
     1201 cgtttgtaat cgtctttgaa gtacctgagg accacatcgt ccggcaggta tcggattgcc
     1261 ggtacgaagc gtatggtatc aacagtaatt gaatctttcc acaccgtatc gggtctggta
     1321 atgttttcca tgcctgtttg aaatgatttt tcatcaaacg ccagcgcctc acctttttgt
     1381 agaaaataat cgcggttgag gtcgttcaga gcaaacaccc gatagatacc attacgtaca
     1441 ttggagacgg taaaacgacc ctgttcgtcg gtacgggcaa ttctcagaaa cggttttttg
     1501 agaaatgcac tatcactgag atcgtcgtga atacccacgg tgataccttg caggggattc
     1561 aggtcttcgg cattgagcag tgtaccggct atctgcatgg tatcaatttc atttccggtg
     1621 gcaaatgaga atacgtaatt tttcagaatg ttgccctcgt tgttgtccac aatagcattc
     1681 ccaaagtcaa ttgaataggt ggtattggat tgcagggtat catcaaaccg aacgttcact
     1741 tttctgccat aggatgtaat ttcgggatta atccgctgag gaggagatat gatcacattt
     1801 ttggaaatgt ctttcaggtt tacattttcg tcaaactcaa tctctatttt accatccttt
     1861 acgtttaccg atttttcgcg gggagttgat tttacaaacc tgggtggtac ttcatccttg
     1921 gcgccaccct gcggtccctg tccacgattg gcacatgcat aaaacaccag tagcaaagtt
     1981 aacgagattg ttatattctt aaattgaaaa aagttcttca ttgttgattg aaataattac
     2041 atttttttaa aaaccttatg taacgaaatc tgttatgtca gttgtacctg attattgctt
     2101 ttactgagca ctttatgccc tatccggcat cgcaggcact tcttatcgtc acaatatttt
     2161 tttttgaggt gcaggagtgc ctgcgaatca aaagctgatg ctgaacttac tccgagttcc
     2221 acccatcgac gaacaaccgc atttttttcg gaagggatca gttccaataa cttaagagct
     2281 gtttcgcatt cggcattttt cttttttgaa tgtgcaaaca aaaacggaac aacggtattt
     2341 atcagtagaa tatcgattga tttccggcct aaacgtttgg ctgactcagc actttgtttg
     2401 ccgaaaagat aatgtttctt ccagtaatcc gatactacgc aatcaaacaa ggctctcaat
     2461 tgcatgatgt cagcggctgc aatcgttttt gagaatagct ttgaagagcg atgagtgagc
     2521 gcagcaaact gagccagcct gacgtgagga aagttatccg gccgaagtct taacagtttc
     2581 cactccgaac tttcaatggg tttcaggtga tattttgttt gcaaaaaacg atattccttt
     2641 tttaatttcg catggtattc atcgttttct gcctgttcga gaagtccggc ctggccgaaa
     2701 agcaacgctt ccaactgaaa aatgtcatct ttgtgttttg ccagaatact catcggcagc
     2761 gaacgggcta atctttcaaa cgcctgactg ttggtgccaa atccgaagct tcgggccagt
     2821 gaaacgtaaa atgcttcttc ccagtggttg tttgtctggg tgagcagggt ctcaatcatt
     2881 tcggtctttt gctccattcg ctcaatgagc aatgcgcttt tccagtcgtt gatgagtatc
     2941 aggggcactt gtgtaatttt atccccgcat ggaatccatt ttttcccgct caacaattca
     3001 ttgtagttca tccggatatg ctccggcact tcgagttcga gctgcggtag cggatcgcca
     3061 ttggttcgaa acacggcttt atctgcctta ttcaccacat gcaggatcac attgtcatac
     3121 gccttatcgg ccgtatgccg gtgtttagcc caatcggaac tctggttgtg aatttcaata
     3181 tttcctgccc agagtgtatc gtctatttta atctttgcat tgaaaaaatc aggtccggca
     3241 tccgtattcg gaagccccgg atggataatc tccaccattt taccgtcagt ggttttcaaa
     3301 ttattcatcc ggaataatct gaattgccag acatagtgta agatagattc tttcatgggt
     3361 gccaaaccat taaagtggta aattataatg tattgaaaat tattcggaac taagtttaat
     3421 ctgtttttat ttcagcattc agatttctta aaaaaaacga tatttttaac ggctttatat
     3481 caacttttat ggattggcaa ttcatattgc ctttacttcg tacccgtttt ctttccagaa
     3541 tttcaacgca tcaggcagcg ttgccaacac attgctatgt gctttctccg aatcgtgaaa
     3601 aacaacgaca gagccacttc tgcttctttt ttttatttca ttcagtattt cagcgggttt
     3661 tactctccgg tcgaaatcgt acgtgatgaa atcccacatg attatttcaa aatccttttt
     3721 cagcttgttc acctgcctga aagttattct tccgtgtggt ggcctgaaca ggctgctttg
     3781 aatcagctca tgcgctttcc atacattttc ggcatattcc cgtacagatg ttttgtgtcc
     3841 tctcaaatga ttgtaggtgt gatttcctac ggaatgtccc cgttttttca cttcctgaaa
     3901 aattccggga aatttcatta cattttcgcc tacacaaaag aaagtggctt tcacgttcca
     3961 ttggtcgagt atatcgagca ctttgggtgt tacctgagga ttcgggccat catcaaaggt
     4021 aaggtaaatt actttttcag ctgattttat tcgccaaacc ggagttcgcc aaagagcttt
     4081 aattatttcg ggaacctgcc gcatttgttt cgatgaaaaa taaaagtttt ccgccaatta
     4141 ccgcatattg aatttattgg caaacatttc aaactgcttc atgtatttat ccagtaaggt
     4201 ttttgaacct gcctgactgc ttatctgcag cacctgattc attacagcca tttcatgtcc
     4261 gattgaactt gtagcgttac tttgctgcga tggtttcagg ctggcatacc agttaagata
     4321 ctcaacacta ttgcccgcta ccgaatcgag tatggcgtcg gcttctgatt tcattcccag
     4381 ttgataatag taaagagcca tcatatttga actgtagtcg tgaggtacgg tagcagcagg
     4441 tatgacttta ttggcatatt cgagtacctt tttagccctc acggtatccc cttcttctat
     4501 taaggcttct gccagataca tgaattgaat ccggtgtgtt ttgcacatac gtaatacctg
     4561 atcgtcgagg taaatttttg gattatctac tccgccaaac ctgaatttat tcatcatgtt
     4621 gtcatacatt ttgtcagtat tcacatttac gcgtgtactg tcctgctgcc cgacgggcag
     4681 aatctgatac accaaaccgg ttctggagag gtatttgctc aaactttgcg gatagaattc
     4741 ctcacccacg gtgacgcata cgtacatggg tcgtttccag ttgttttcct tgatcatttc
     4801 catgaccatg gcttcgcctt tgaaaacact tttgggcaaa tcaaatatca tttctggtac
     4861 aattttagaa atatctttag caggaactga acctgttttc accacttcag cagcatctac
     4921 cggcagcgag agtttcggag aggggaagaa tccatctccg tcaattttga attcagcttc
     4981 gtcgcttttg ataatttcaa aggcatcttt catgttgaat ggttttccat cggtcagatt
     5041 ctgcactgac atcaaatcgt ttctacctga aatgtaatct ttcttttgcc aggaaatagg
     5101 cagcggtgcc gattcgtaag cctgacgttt catctgatcg atgtaccaat cggttggcag
     5161 atagctgagg ttacatactc gcacatcggt tcgtacgcct tctacctcct gattgtacca
     5221 aagtgggaac gtatcgttgt cgccgtttga aaagaggatg gcgttttttt cggttgagtt
     5281 caggtaattc tgtccgaaat cacgggctgt agtgcgtccg ctgcggtcgt ggtcgtccca
     5341 gttttgggca gccatcagta caggaatacc caggcaggca atggcagcta tggcagcact
     5401 tacatttttg ggtaatatct tatcgagtaa ttttgacagc cccatgacac caaggcctat
     5461 ccagatactg aatgcataga acgatccggc ataagcgtag tcgcgttcgc gcggctgata
     5521 gggtgtttgg ttgagataga ccacaatagc cagtccggta aggaaaaaca agagcattat
     5581 tgcccaaaaa acatgatttc cctgtttacc ggcattgagg aggaaaaaaa ttccgagcaa
     5641 tccgagcagc agcgggagca taaaatagac attccgtcct ttattgtttt tcagttcgga
     5701 aggcaatagt ttttgattgc ctacaagcat gttatcgatg aaagggatac cggtaatcca
     5761 gtttccgtgt tcaatttcac catatccctg catatcgttc tgtcgcccgg aaaaattcca
     5821 catgaaataa cgccagtaca tgaaattaac ctgataatca aagaagaacc gcatattttc
     5881 accaaaagtg ggtttaatat cgctcctttg ctcgccgcaa aaatcataat tcactttttt
     5941 gcccacaaca ttcccccaca ttttgtatgc tttcacatga ttcggatcgc cgctgtacat
     6001 acgtggaaac aacatcatga atttctcgtt tttcttgtaa tcgaatttat aacctgccac
     6061 aacatattca tccttttcag aagcatcacg tttgggtttc ggagcataaa gaggtgtttt
     6121 atcagcatcg tacgtgcagg tatttccttc cactttgagt ttcatgggag cgttgtacac
     6181 gccgccgtat agcagcgggc gatcgccgta ctgctgccgg ttgaggtaat actgaagtga
     6241 gaacacatta tctggtgaat tctgatccat ggtaggctgg gctgccgagc gaatcatgat
     6301 aactgcatac gaagaataac caatcataat cacagcaatc attaccaaac tggtattgag
     6361 ccagcgtggc ttcacggatt tctttttata agcaaagaaa cctatcattg ccaggatgat
     6421 aaagatgccg agtattatat tttcgaacag gaaaggcaca cccgtgatag taaccgccag
     6481 gataaacgaa acagccattc tcgtgtagtt tttgcctgtg tgggtttcgt aaattgccca
     6541 gaccagcgct gcaataacca taaaaatgta aacaaacaaa cctgtattat aagaagctcc
     6601 taacacatta acgaatagca attcaaattt ggtagtccac atgatagtgc ccggaatgag
     6661 tccgtaaagc gtaaatgcaa gaatgatgac agaagctgcc attgccagta aaattcccct
     6721 ggttgaagga gtgtattttt tgtaataata aaccagtacg atagccggta tagcgagcag
     6781 gttgagaagg tgaacgccaa tagaaagccc catcaggtaa gctatgagta tgagccagcg
     6841 atcggaaccg ggattatcgg catttctttc ccatttcaga atgagccaga aaactactgc
     6901 tgtgaagaac gatgaaaacg cgtacacttc accttctacg gccgaaaacc agaaggtatc
     6961 cgagaaagtg taagccagtg ctcccaccag tccggctccg atgacaatta ttttattggc
     7021 tgtagtgtat tcttcttcgg attttccgat taccttctga gccagatgtg tgatggtcca
     7081 gaagaggaaa agaatggtaa aggcacttgc cagcgccgac atagcattta ccatcatagc
     7141 tacctttgaa ggatctgaag taaaaagcga gaagaattta gccgttagca tgaagaaagg
     7201 tgctccggga gggtgcccta catcgagttt ataggcagct gagataaact caccacagtc
     7261 ccagaaactt gcagtcggtt ctattgtcat cagataagtg accgctgcca ctgcaaatgc
     7321 cagccagcca aaaatggtgt tcaaaaatct gaaatttttc atcggatttg aattgtttat
     7381 gttgtatttt gtactttgtg ttgtattttg attcaaaaaa aacgttcaaa gatacaaaga
     7441 aaattcagtt tgagggtaaa gctatatgca ttttagagaa tattaaaaaa acgacagcca
     7501 ccatctggtt gatagcagct gtacgcgaat ttttcaaaaa acaggattct atcaaaaaat
     7561 cattgttctg taatcgttac tccggcgggt atttcaaatt tttcggcagg cggagttacc
     7621 gcttcgattt tggtaacttc gatggaggtc gggatttcca tcacttttcc tttcattttc
     7681 atcatcagat tgccactggc gaggtattcg acgtccattt tcatttcgtc gtttttcatt
     7741 ctgtatttta cgcaggggaa gcccaggtaa tcttcctgac caattttttc cagtttcatt
     7801 ttacccatgg tttcttcggt gagtgtttcg aggtcaactc ccaattggtt gataaccgct
     7861 gcgcggttga aatgaaatcc ggtttttttg tccatgtcaa tatcccagtt atcattgcct
     7921 tttacaatgg taatgttcgt tgaactcatt ttctggccga gcatttccat agtggtagtg
     7981 gtttcggtgg cttcccattc gccccattta tcgaaataaa tgaccggatt gctttctatg
     8041 cccattacat ttgatttctg ataaacaatt cctttttcaa tcgggtattt tttacctttt
     8101 tttgcctgtg ctgctacttc tttggcagca tctttgtcgg ttttcgattc gccggtttgc
     8161 ttttgcccac aagcagcaaa cacgaataag gcactgagaa taaaaattaa ttttttcatg
     8221 atctgaagtt tttttaaggt aattaatttt ggcagttgat tcataaagta ttgcggagtg
     8281 ttattctgcg ccactgtcca gccggcagcg agcagaacga cacccagtaa cttggttttt
     8341 agtgacaatt taatttctat tgttttacaa attccaccct gcgattttta gctttgtttt
     8401 cgggagtatc gttggcggca acaggttttg tctcgccggc tccttcagta atcattcttg
     8461 tagcatctat accgaagttt ttggacagtt cgtttttcac ggattcggct ctacgcctgg
     8521 ataagtcgag attttttgct gcatcgccgt cactgtcggt atgtccaatg attttcactt
     8581 tcacagtcgg attttcggac agcacatcgg ctatgctttt gagcgttccg tacgattcgg
     8641 gcttcacatc ggctttgttc acatcgaaag taatgccgta ggttacgagt ttaccttcgg
     8701 tgatgagctt gctgcgggta tcgggcgatg cggtggtgat tttcaggttg gtcaccatgg
     8761 gccagcttct cacgtcccat cccgaaaatc gaattttgtt gaaagctatc cccggatata
     8821 tgttggttgg catatcgagc actttggctc cctgatgata gattcttacc ctccttttct
     8881 gtatccagat aatcacatga ttgacttttt cggtcaccac aggatttttt gaggcttctc
     8941 cggttagcca gcggtcgtcg ggactatcgg tatatccaac agtgctccat ccttcctttc
     9001 cgggtacgat gtgtagtcct cctttaccgg gatacaaatc atcgttgagt tcctgcggat
     9061 cttcaggatt gtcctggtag atcgtaaagc ggattccatc ataatattcc gcatcgggta
     9121 tgatatcaaa ttccacaatg aaattatcgg ggaaggagat gtttttagtg taacaatata
     9181 ctgcattttc gccgttcatg tggaaccagt tgccgggagc tatgttcact gttttcactt
     9241 cgccgctgcc gttggttgtc cacagtgccg ggaaatcgcc taaggcatcc tggctgaaat
     9301 cttcgaaaaa gaggatttta tcacccggca caaagtcgta tgcagtaact gattcgagtt
     9361 tctgcttatt actactattt gttacagcat cttcgtcctg attttcgtca ctttctgctg
     9421 cttcttcagc atctttctgt cctttttggt ttttcttacc cgattttttt accgatcctt
     9481 ctgcattatc gagcacttta tcggttgctt cgtcggtttt ttggtcaata cgattttcca
     9541 ccttctccac cacacggtct tttacccgtt gaccggcccg ttcaagccaa ccctgcgcac
     9601 tcatttgtaa accaaacaaa aaacagatac aaagaaaaaa tacaatacgt ttcatgatta
     9661 taaattttgg tattagtaaa aaatggttga atgctgattt taaaagtcat ttttcatcct
     9721 gaaggaaaga ataaaaaatc gattttaata gtgaattccc gatgtaatga atatcgacga
     9781 aagaatagct gagagtacca ctgagccgat aattacaacg atgagactta ccacaaaata
     9841 ggttgttttt ttgtcatcgg gagttttttt catgggttga agaccgatat agagcaggta
     9901 taacccatac aaaccggcca acgtggcaag aatgcccagc gcaggaataa tcagaaaaac
     9961 acctgccacc atcatgggtg tataggaata tacaaccagt ttgaacgatt gcagaaagtt
    10021 cttttcggag ccgaagcttg gtgccagtaa atcaatgatg taagcggtaa gaaaagctcc
    10081 accgatggta cttaccagac tgacgatggc ataacgcaga ccgtatccgg ccgatccgat
    10141 gtgcatacca agtacatttt gtcctatcag tccaaagccg ataaatccgg ctatggcagg
    10201 aatcagagcg agaggcaaca agtagttgag ggtcagtgtt tttacatctg tttgttcggg
    10261 ttctattacc gcccattcgg tttttggcga caacagaatg cttttagcac gattaatcag
    10321 gttcatagga ataataattt aataggttta tgaattaaga aatatgaaat tagctaaaaa
    10381 gttgatccaa ttaattatta gtcagactct taaccggaaa aaagctactt atttccggtt
    10441 ttcaacactt tctcatcaat ttttgttaca aaaacctctg ttaattcttt ctatttttcc
    10501 acgtattatt ccatcgctta acgcgttggc aagacaaaca acaaacctct tatccgactg
    10561 tatttcaaaa atttgaatca cctgttgatg ttgttttgta ttgattttcc atcattgccc
    10621 aaaagttatc aattcctgat caatgcacga aaat
//
LOCUS       DAORKP010000075        10447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_768_length_10447_cov_9.428695, whole genome shotgun sequence.
ACCESSION   DAORKP010000075 DAORKP010000000
VERSION     DAORKP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_10447_cov_9.428695"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2698)
                     /locus_tag="PLN75_05575"
     CDS             complement(113..2698)
                     /locus_tag="PLN75_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03620.1"
                     /translation="MKIRLLLFLFILSFPLFGKITAQDIESANKLKSYAQQYVAFSKN
                     YPREKVYIHFDNTSYFLGETIWFKAYVVRADRHSLSELSKVLYVELINQEGYVVAEKK
                     LKIENGQCHDNFYLPSAGYAGFYEIRAYTRYMLNFGNENYFSRVLPVYDEPKNEGEYT
                     PKVTVRPNSQRIPLKRPSYSQKERISLTFYPEGGNLVQGVKSKVAFKATGENGENVIV
                     SGNIVNGKNEVLADIYTEYLGMGAFEYTPTAGRQFAKVSYNNRDYTFQLPEALPEGYV
                     MNVNNLDSAKVNILISKSPNTKSEPLGLSVSCRGIMYAFENVDLTTDNEINLTLSKNM
                     LPSGVSQITLFSPKGEIYSERLIFINHQSEMKINMTQNKPVFRPYEEVTMNFRLNDRK
                     NNPVQTTFSLSVCDAGTSSVVPYADNALTNLLLSSELRGYIENPGYYFETDDLRRKSA
                     LELLLLTQGWTRYEWKEMSGAAPFNPVHPLEKELAIEGSVVSLFRKTELKDVEIMMVL
                     MGDSSSQRGKALTDENGKFGFLLRDFYGDAKIILQSKIDEKRKETRIMLDRNFSPDLK
                     SFSQTEINEPRHMISFNGSETAESNVDTQYENDTINGDLPMSEKMHVLKDLIVTARQR
                     PIKISVKYEVAKEMDKIQDTGEWSPADIYSFLDKMNQYFSYSTDSGGSTFRYKGKKVY
                     FMRDDSPDLVAFNIESLMSGQSTDLVSTQNEINTSMNGSNNNFNTQMPVFEEIESITI
                     IEDYGSILRLFSGDPNYDPTKVAIVVMHLKKNYQKEPVGIRNTSFRGFSYPKAFFNPL
                     YNNRDLPQLTEADYRRTLYWNPNVTSDSSGNAEISFYNNGKSRHFNVSLETVTQNGII
                     GSFKN"
     gene            complement(2781..3809)
                     /locus_tag="PLN75_05580"
     CDS             complement(2781..3809)
                     /locus_tag="PLN75_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03621.1"
                     /translation="MMKELKDLAAHPFDSKVRSNVQNYIYFSAPLLGQGTLGCYAPCA
                     DISILYISCRATEDFIWKNNINSRKSDAFYFNYFLKVSHGLQAMSEDRKSKRSLVENT
                     LLVDGTLSAEKSLILVKDEIFEIFQVALSTAYLESLLNEYLTDFTPEIINFCYRKSTD
                     RSQVGLTLPIESREEMCIREIVNCPLEINLQNEYTVMKVKELILYYLQRMVNGGVLEV
                     SDNKDISVREKEKLINVKTFLEQETGKDPDYGHLCELAEMNKQKLNVLFEKVFGQSIA
                     RYHRTQKMQRACNLLLDFEKKTEYQRDSLSTWIFVSFIFFKSILQRIQKKTYRIQNRI
                     TTILTYIN"
     gene            complement(3821..4903)
                     /locus_tag="PLN75_05585"
     CDS             complement(3821..4903)
                     /locus_tag="PLN75_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRG03622.1"
                     /translation="MSLYSDLRKDDYIEELSISMSDFDEILPSKTPNFFNFKSASIGN
                     GTFGSYTPKKGVWIFYASLFPEKDVVWNSAIIEDGKDTLFFNYILNAPEGMRSYVEIP
                     EAEETLSSKTLLIDATKSSRKSLKLPGKKKFEILQIALSEEIFSDYMMELIPQTTDEI
                     KKYIQSHFYKKNQTGLMLPLDIKEERCIREIIHCPTKEELQRFFTLLKISELMMCYFS
                     KIINKEILNIIGGEKITLTEKDTIVKIKKHIDENIEKDLDVNALSELSGMPATKIKSL
                     FCYIFGCTISKYHRKKKLNEAYSKLLDIGNKMTVKEITFALGFNSIASFSRSFYNEFK
                     IRPSDINVKIRDLDAFMHQTKKSAND"
     gene            5253..9089
                     /locus_tag="PLN75_05590"
     CDS             5253..9089
                     /locus_tag="PLN75_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRG03623.1"
                     /translation="MIKTTTFQLKTITLTVMLVCFGVIPAFGQLTITKEAPDSVKPGD
                     ELRFRIVYQNNGTTIQNNVVIEDVLPSVDFTYLYSDPVGNFDPLTNKITWTKNEIPEL
                     EHLNAGTHVIHVYGRVGKKFIDASHYPDGYYLSNSTSQDFFNAATIKSDQITTPITSN
                     TTKSSSKQKCTVTLNQASGVIKSASNSELFYIVSVTNTGNIWNKWVLSTTQAGNPFQQ
                     LTTSFSSLNGIPLNNNSTEWIEPGATSMYLMKLVSPIGTNPSVGTPQDPNKTTVTATP
                     VACGNPVSKEFITEICGGNCPPYQYVSAYKVDVPDPVQSGGNLEYRMIIYNSYGSPVQ
                     NITLTETYPLLTTYISSTQPTNNGGAVPHTFTGNNKWTFNNLPAGLTTFNVIVKVNDN
                     VTPNTTIENKIELTTPAYGTAPFLTFIEKTQVVSTHDLWITKTANKTNAVAGDIITYT
                     LNFGNMGNYKGDNVTITDNYDETYMDVIDAAGGTNSNGNIEWTISGDMPIGSSYTKTY
                     QLQIKPGANFPAGTTNIHNMAYIANHQNPLISFDSDYTNNQAGYKVFVVNIPDLKVVK
                     IADKNRVSPNENLTYTITVSNIGDADHTGNYTVKDFLPIGVNYVSSSPSGDYNTSDHS
                     VIWTFSTPLVINDSHTLSLEVSNITEDMGGTDLVNEVTVYSAGMNDKDLDNNEFSLSV
                     PVAINLWYGGVSTDWAVETNWTYGVVPAAGENVVFATTVNHTSAAINDLMLDQDRTIG
                     NLVNESDKALIIPVEKTLIVNGTATMGTADQLILKSLKTKANGALVFAQPHNNAAVEA
                     TVEFASISKPSTGNWPRIWQFFGVPVRNKRLFELFGPNVQGSIYGGDPSVNTIIRKYE
                     EYHTDPQSLQEKWQNVAINDILVPFLGYEITQPQAAFDNADTSPYKFRGLLNTDENFS
                     LPLTISQSPVYSRGNYMLANPYAAPIFISNMQAGDFDNLAPTIYIYNTGSRQDWLTNN
                     GAGQAGDLPGTYSAIPINAANTIGKTQIPSMQAFLVKAIADNPPTANFTFRYATVYRN
                     AITTQNEPMRISGVLSEKTIDVTSKQIKPMLTIDVIGENGSDRVYLITAEGTGKSYDA
                     GWDGYKTLSADNVQLYAMDADNRRMQVNTDNDLNDTYIGFRTGGESTYTLKFRFNTEM
                     QGMYESLNIQDLATGFTQQIADSGSVTFASSAGSAEKRFKLTGNRVITGTKPQPENSD
                     IQLTVKPDNIDVRNNTGEELLVTVYDLAGHLMMTNKVPTGMMSIRHDLKPGIYMLEAY
                     AKVSGQKTILKSVINK"
     gene            9100..9744
                     /locus_tag="PLN75_05595"
     CDS             9100..9744
                     /locus_tag="PLN75_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03624.1"
                     /translation="MKTKIGTIALLTFTVLSLSFLLLRSTIHSIKEEALIESFNKVRP
                     NTQQVLAGTIEVPAYKPRNKSSKSIYSGNTEGFSSFSSSESYSGGVGNDPEIFSTTAV
                     ISSNGGKSSLGHRAGNNAEKSIPSAMIALKQSKSSGSERLYASNRVSVRVEAASGLTQ
                     PFSGGGVSGPLMRMDGDDYNPPAEGAPVGEGVVILLVLSVAYGVYQRFRVKSVF"
     gene            complement(9824..9906)
                     /locus_tag="PLN75_05600"
     tRNA            complement(9824..9906)
                     /locus_tag="PLN75_05600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9871..9873),aa:Leu,seq:taa)
     gene            complement(10044..10116)
                     /locus_tag="PLN75_05605"
     tRNA            complement(10044..10116)
                     /locus_tag="PLN75_05605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10081..10083),aa:Gly,seq:gcc)
     assembly_gap    10147..10246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2987 a         2104 c         2125 g         3131 t
ORIGIN      
        1 ccacgtgttc aacattaaga tctgattgac agatgtgtaa aaaaaaatga acagaggagc
       61 aatcatctgt tcattttttt tatttcatcg ggtacatgaa gacctgattt ttttaatttt
      121 tgaaagatcc gataattccg ttttgagtta cggtttcaag gcttacgttg aaatgcctgc
      181 ttttaccgtt gttatagaat gatatttcag cattaccgct actgtcggag gttacattcg
      241 gattccagta gagggtgcgc cggtagtcgg cttcggttag ttgaggcagg tctcggttgt
      301 tgtacagcgg attgaaaaat gctttcggat aggagaaacc cctgaacgag gtattgcgta
      361 tcccgacagg ctctttttga taatttttct tcaaatgcat taccacgatt gctacttttg
      421 tggggtcgta atttggatct ccactgaata accgcagtat cgaaccgtaa tcttcaataa
      481 tagtaatcga ttcaatttct tcgaacacgg gcatttgtgt gttgaaattg ttgttgctac
      541 cattcatcga agtgttgatt tcgttttgag tggaaactaa atcggtggat tggccgctca
      601 tcaggctttc aatgttgaaa gccaccaaat cgggcgaatc atcgcgcatg aaatatactt
      661 ttttgccctt gtacctgaat gttgagcctc cactatcagt agaataggag aaatactgat
      721 tcatcttatc cagaaagctg tatatgtcgg ccggactcca ctcgccggtg tcctgtatct
      781 tatccatttc ttttgccact tcgtatttga cgctgatttt tatcggtcgt tgtcgggctg
      841 ttactatcag gtctttcagt acatgcattt tctcgctcat gggcaaatcc ccgttgatgg
      901 tgtcgttttc atactgtgta tctacattgg attctgcggt ttcgctgcca ttgaatgaaa
      961 tcatgtggcg gggctcgttt atttcagtct gagaaaatga cttcaaatcc ggactaaagt
     1021 tcctgtcgag catgattctg gtttcttttc gcttctcatc tatcttcgat tggaggataa
     1081 tcttggcatc gccgtagaaa tcacgcaaca gaaaaccgaa ttttccgttt tcgtcagtga
     1141 gtgctttacc ccgttgcgag gagctgtcgc ccatcagtac catcatgatt tccacgtcct
     1201 tcaattccgt ttttctgaac aatgagacca ccgaaccttc gattgcaagt tctttctcaa
     1261 gcggatgaac cggattgaat ggtgctgcgc cgctcatttc tttccactcg tatcgggtcc
     1321 atccctgagt aaggagcagc aattccagtg cactttttct cctcagatcg tcagtttcaa
     1381 aatagtaacc cgggttttcg atatagccgc gcagttcgga cgaaagcagc aagttggtca
     1441 gtgcgttatc ggcataaggc accacagagc tggtaccggc atcacacaca gaaagcgaga
     1501 atgtggtctg aaccggattg tttttcctgt cgttcagccg aaagttcatt gtcacctctt
     1561 cataagggcg gaaaacaggc ttgttttgtg tcatgttgat tttcatttca ctctggtgat
     1621 tgatgaaaat cagtctttcg gagtatattt cgcctttcgg actgaaaagc gtgatttgtg
     1681 acacacccga tgggagcata tttttagaga gtgtgaggtt tatttcgttg tcagtggtca
     1741 gatccacatt ttcaaaagca tacatgatgc ctctgcacga aacagaaagc cccagaggtt
     1801 cggattttgt gtttggagat ttactgatca ggatatttac tttggcgctg tccaggttgt
     1861 tcacattcat cacgtaacct tcaggtaagg cttccggtaa ctgaaaagtg tagtcacggt
     1921 tgttgtagga tacttttgcg aattgcctgc ctgcagttgg agtgtattca aatgctccca
     1981 tgcccaggta ttctgtatag atgtctgcca gcacctcgtt tttcccgttc acaatatttc
     2041 ccgaaacgat tacattctca ccgttctctc cggtggcttt gaatgctact tttgatttca
     2101 ctccctgtac cagattacct ccttcaggat aaaatgtcag tgaaatgcgt tctttttggc
     2161 tgtaggaagg tcgtttcagt ggaattcgct gtgaatttgg tctcacagtg actttaggtg
     2221 tgtattcacc ttcgtttttg ggttcgtcat atacaggcag caccctggag aaatagttct
     2281 cattaccgaa attgagcata taccgtgtgt atgctctgat ttcataaaaa ccggcataac
     2341 cggctgatgg aagatagaag ttgtcgtggc attgcccgtt ttcaattttc agtttttttt
     2401 cggccacgac atatccttcc tgattaatca gttctacgta cagcacctta ctcagttcgc
     2461 tcagtgagtg gcggtcggcg cgcacaacgt aagctttgaa ccagatggtt tcacccagaa
     2521 aataggaagt attgtcaaaa tgaatgtaca ctttttcccg cggataattt ttcgagaatg
     2581 ctacgtattg ctgagcgtag cttttcaatt tgtttgcaga ttcaatgtcc tgagcagtga
     2641 ttttaccgaa aagcgggaag gataagataa agagaaatag aagtaacctg attttcatag
     2701 taccatttcg aatttgatct tcaaaggtac aaaaatatat ttttcagagc gggtaatttc
     2761 tgaaagaatt gtcggtaatt ttagtttatg tatgtcagaa tggttgttat ccgattctga
     2821 atccggtagg tctttttttg aattcgttgt aaaatgctct tgaaaaagat gaaactgacg
     2881 aaaatccaag ttgaaaggct atctcgttga tactcagttt ttttttcaaa atccagtagc
     2941 aaattacagg cacgctgcat tttttgtgtc cggtgatagc gcgcaatact ctgcccgaaa
     3001 actttctcaa acagtacatt cagtttttgt ttgttcattt cggcaagttc gcacagatgc
     3061 ccgtaatcgg gatctttccc ggtttcctgt tccagaaatg tttttacgtt tatcagtttt
     3121 tctttttccc ggactgaaat gtccttgttg tccgaaactt caagcactcc tccgttgacc
     3181 atccgctgaa ggtaatagag tatgagttct ttcactttca ttacggtgta ctcgttttga
     3241 agatttatct ccaaaggaca atttacaatc tcgcgaatac acatttcctc tctcgattcg
     3301 ataggaagag ttaatcctac ctgacttctg tcggtgcttt ttcggtaaca gaaatttatg
     3361 atttcggggg tgaaatctgt caggtattca ttcaaaagcg attcaaggta agcggtggaa
     3421 agtgccacct gaaaaatctc aaaaatctca tcttttacaa gtatgagtga tttttcggca
     3481 gaaagcgtac catctaccag caacgtgttt tcaaccagcg accgtttgga tttccggtct
     3541 tccgacatgg cctgtaatcc atgcgaaact ttcagaaagt aattgaaata aaaggcgtcg
     3601 cttttccggg agttgatatt gtttttccag atgaaatctt cggtcgccct gcatgagatg
     3661 tatagaatgg aaatatcggc acacggagca taacatccca gagtgccctg cccgagaagc
     3721 ggagcactga aataaatgta attctgaaca tttgaacgta ctttagagtc aaacggatgc
     3781 gcagccagat ctttcagttc tttcatcatg tgtgttttat ttaatcattt gcagattttt
     3841 tggtttgatg cataaatgca tccaaatctc ttattttcac gttgatatca gaaggcctga
     3901 ttttgaattc attataaaac gaccttgaaa acgaggcaat tgaattaaat cccagtgcaa
     3961 acgtaatttc ttttacagtc attttatttc cgatgtcgag gagtttgctg taggcttcgt
     4021 tcagtttttt cttccggtgg tacttgctta ttgtacagcc gaaaatgtag cagaaaaggc
     4081 tttttatttt tgttgccggc atgccggata attcacttaa tgcatttaca tccagatctt
     4141 tttcgatgtt ttcgtcgatg tgttttttga tcttcacgat tgtgtctttt tcagtcagcg
     4201 taattttttc tcctccaatg atgttcaaaa tttctttgtt gatgatttta ctgaaatagc
     4261 acatcatcag ttcactgatt ttcagaagag tgaagaatcg ttgtaattcc tcttttgtag
     4321 ggcaatgaat tatttcgcgg atacatcgtt cttcttttat atcgagcgga agcatgagtc
     4381 cggtctggtt tttcttgtag aaatggcttt ggatgtattt tttgatttca tcggttgttt
     4441 gtggaatcaa ttccatcatg tagtctgaga aaatctcttc ggataaagca atctgcaaaa
     4501 tctcaaactt ctttttccct ggtagtttta atgatttacg ggaagacttt gttgcatcta
     4561 tgagcaatgt tttacttgat aaagtctctt cagcttccgg tatttcaacg tagctacgca
     4621 tcccttcagg agcgttaagt atgtaattga aaaataaagt atcctttccg tcttcaatta
     4681 ttgcactatt ccatactacg tctttttcag gaaaaagact ggcgtagaaa atccatacac
     4741 cttttttggg cgtataagac ccaaaagtcc cgttgccaat ggaagccgat ttgaaattga
     4801 agaaattagg tgtttttgac ggtaagattt catcaaaatc agacatcgaa atactcaatt
     4861 cttcaatata atcatcctta cgcaaatctg aatataaact cataatgtca tgtatttgcc
     4921 tttgggagaa gctttcgttt atttccggtt gcaaatgtaa caaagaaatc ataaatggat
     4981 ggcagaaatc agatgtttta ataaaataaa ggttaaagat gtataattaa taggtttgag
     5041 gatagaataa atcgtgtata attcataaaa acggaatata agattctgaa atggtaacaa
     5101 aaaagtcaaa aatgataaca gattattttt atcacccttt atttttgcgt ggaataattc
     5161 taaataaata caacgaaatt tagttctcat ttttttagca ttttaagcct gaaccttcat
     5221 tttttcattt acacattaaa aattagaatt gtatgataaa aactactact tttcaattaa
     5281 aaacgattac attgaccgta atgcttgttt gttttggtgt aatccctgct ttcgggcaat
     5341 taacaataac taaagaagct cccgattccg taaaacccgg cgacgagttg agattcagaa
     5401 tcgtatatca gaataacgga actaccatac agaataatgt ggtgattgag gatgtgttac
     5461 cttcagttga ttttacttat ttatattcag atcctgttgg caatttcgat ccgttgacca
     5521 acaaaataac ctggacaaaa aatgagatac cggaattaga acatttgaat gccggaacac
     5581 atgtaattca tgtgtatggc cgggtaggga aaaaattcat agatgcttct cattatcccg
     5641 atggatatta tttgtcaaat tccacttcgc aagacttttt caatgcagcg actatcaaat
     5701 ctgatcaaat tacaactccc ataacaagca atacaacaaa atcttcatca aaacaaaagt
     5761 gtacagtcac actgaaccag gcaagtggag tgataaaatc agcttcaaat tcggagttgt
     5821 tttacattgt atctgttacc aataccggta atatatggaa taagtgggta ttatctacta
     5881 ctcaggcagg aaatccattc cagcaactta caacttcatt ctcatcacta aacggtatac
     5941 cgcttaacaa taacagtacg gaatggatag agccgggtgc aacttcaatg tatcttatga
     6001 aactggtttc gcctatcggc acgaatccca gtgtcggaac tcctcaggac ccaaataaaa
     6061 ccacagttac tgccactccg gttgcatgtg ggaatcccgt atctaaagaa tttataactg
     6121 aaatatgtgg aggtaattgt ccgccatatc aatatgtaag tgcttataag gttgacgttc
     6181 ctgatcctgt acagtcaggc ggaaacctgg aatacagaat gataatctat aattcttacg
     6241 gatctccggt tcaaaacatt acacttaccg aaacttatcc attgctgacc acttacatca
     6301 gcagcacgca accaactaat aatggtggag cagtacctca cacatttacc ggaaataata
     6361 aatggacatt caacaacctt ccggctggtc tgacaacttt taatgtcatt gtgaaggtga
     6421 atgacaatgt tacaccaaac acaaccatcg aaaacaaaat tgaattaacg accccggcat
     6481 acggaacagc cccatttttg acttttattg agaaaacaca ggttgtatct acccatgatt
     6541 tgtggattac aaaaactgct aacaaaacaa atgcagttgc aggtgatatc attacatata
     6601 cgttgaattt tggtaacatg ggtaattaca aaggtgataa tgtaactatc actgataatt
     6661 atgatgaaac ctacatggat gtgatagatg cggctggtgg aacgaatagc aatgggaata
     6721 tcgaatggac gatatcagga gatatgccta tagggtcaag ttataccaaa acctaccagc
     6781 tgcagatcaa acccggtgct aattttccgg caggaaccac caatatacat aacatggcct
     6841 acattgccaa tcatcagaat ccgttgattt cttttgattc tgattacacc aacaatcagg
     6901 cgggatacaa agtatttgtt gtgaatatac ctgatctcaa agttgtgaaa attgctgata
     6961 aaaatcgagt gagcccaaac gaaaatttaa cttacacaat aacagtatca aatattggag
     7021 atgctgatca taccggtaat tatacagtca aagattttct ccccatcgga gtgaattatg
     7081 tttccagttc accgtcaggc gattacaata catcggatca ctctgtaata tggacatttt
     7141 ctactccgct ggttataaat gattcacata ccctaagcct ggaagtaagt aatattactg
     7201 aggatatggg aggtactgac ctggtaaatg aggttaccgt ttactcggca ggtatgaacg
     7261 ataaagacct ggataataac gaattttcac tttctgtccc ggtcgccatc aatttgtggt
     7321 atggtggagt aagtaccgac tgggctgtcg aaaccaactg gacatacggt gttgtacctg
     7381 ctgcgggtga aaatgtggtt tttgcaacaa cagtcaatca tacttcagca gccatcaatg
     7441 acctgatgct ggatcaggac agaacaatcg gaaatctggt taacgaatca gataaagcgc
     7501 tgataattcc ggttgaaaaa acgttaatcg ttaacggaac agccaccatg ggtacagctg
     7561 atcagttaat actaaagtca cttaaaacca aagcaaacgg agcattggtc tttgctcaac
     7621 cacataacaa tgctgccgtt gaagctaccg ttgaatttgc ttccatttcc aaaccatcta
     7681 ccggtaactg gccaaggata tggcaatttt tcggtgttcc tgtgagaaac aagagactgt
     7741 ttgaactgtt cggacccaat gtgcagggaa gtatctatgg aggcgatcca tcggtaaata
     7801 ccattatccg gaagtacgag gaataccaca ctgaccctca aagtttacag gaaaaatggc
     7861 agaatgtggc aatcaacgac atccttgttc ctttcctggg gtatgaaatc actcagcctc
     7921 aagcagcctt tgataatgct gataccagtc cgtacaaatt caggggactt ttgaataccg
     7981 atgaaaattt ctccctcccg ttaacaatat cacaaagtcc ggtgtactca cgtggaaatt
     8041 atatgctggc taatccgtat gcagcaccga ttttcatcag taatatgcaa gccggtgatt
     8101 ttgataatct ggcacccacg atttacatat acaataccgg aagtcgccag gattggttga
     8161 caaataacgg agccggtcag gcaggtgatc tgcccggaac ttattcggca atccctatta
     8221 acgcggcaaa tacgattgga aaaacacaaa ttccttcaat gcaggcattt ttggtcaaag
     8281 ctattgcaga taatcctccc acagcaaact ttactttccg gtatgctaca gtttatcgga
     8341 atgctattac cactcaaaat gaacctatga gaataagtgg agttttgtct gagaaaacga
     8401 tagatgtcac cagcaaacag attaagccga tgctaaccat cgatgtaatt ggtgaaaatg
     8461 gttcggacag ggtgtatctg attacagcag aaggaaccgg gaaatcgtat gatgcaggat
     8521 gggacggata caagacgttg agcgccgaca atgttcagct ttacgcaatg gatgccgaca
     8581 acaggcgaat gcaggtaaat acggacaatg atttgaatga cacgtacatt ggcttccgta
     8641 ccggaggaga aagtacgtac accctgaaat tcagattcaa taccgaaatg cagggaatgt
     8701 atgaatcgct taacatacaa gacctggcaa ccggattcac tcagcagatt gccgacagcg
     8761 gttcagtgac cttcgcctct tcagcaggtt ctgccgaaaa acgcttcaaa ctgaccggaa
     8821 acagggtcat tacaggcact aaaccgcaac cggaaaacag tgacatacag ctgactgtaa
     8881 aaccggataa tatcgatgtg agaaacaata cgggcgaaga acttcttgtt accgtttacg
     8941 atttggcggg tcatttgatg atgactaata aagttccgac aggaatgatg agtattcgtc
     9001 atgatctgaa accgggaatc tatatgctcg aagcgtatgc caaagtcagt ggacaaaaaa
     9061 caatcctgaa atcagtaatt aacaaataaa ccggaacaga tgaaaaccaa gataggaaca
     9121 atcgctttat taacatttac tgtgcttagc ttatcatttt tgctgctaag aagcaccatt
     9181 cattccatca aagaagaagc actgattgag agtttcaata aagtcagacc aaatacgcag
     9241 caggtattgg ccggtacaat tgaagtgccg gcgtacaaac cccgcaataa gagttcaaaa
     9301 tcaatttact ccggcaatac ggaaggattt tcgtcctttt cgtcttctga aagttattct
     9361 gggggcgtgg gaaatgaccc ggaaattttc tctacaacgg cggttatatc atcgaacggc
     9421 ggtaagtctt cgttaggcca cagagcgggt aacaatgcgg aaaaatctat accctcagcc
     9481 atgatagccc tgaaacaatc gaaatcatcg ggcagcgaaa gactttatgc ttcaaacaga
     9541 gtttcagtgc gggtagaagc cgcttcagga cttacgcaac ccttttccgg tggaggtgta
     9601 agcggacctc tgatgagaat ggatggcgac gactacaatc caccggccga aggagcacct
     9661 gtaggtgagg gtgttgtaat attgttggta ctttctgttg cttacggtgt gtatcaacgg
     9721 ttcagggtga aaagtgtttt ttaatctccg ggctgtttat ttatagcagc gtgatttgtt
     9781 aatgaaaaaa ccttgtaatc agacgattac aaggtttttt ttgtacccga agcgggactt
     9841 gaacccgcac agccgtgaag ccaaaggatt ttaagtcctt cgtgtctacc attccaccat
     9901 ccgggcagga atgattcctt aagaaaaacg aattacttcg ttgtctctgt gagcgaaaaa
     9961 cgggacttcc cgatattgct tcgctgcatc gggataaact tctcgtcccg ttttcagcag
    10021 catttaagct tttcactact ttgagcgaaa aacgggactc gaacccgcga ccctaacctt
    10081 ggcaaggtta tgctctacca actgagctat tttcgcatat ctaaaattag aaattagaaa
    10141 tttctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt ctgccttctg
    10261 ccttctgcct tctgccttct gccttctgcc ttctgccttc tgccttcttt taagcgggtg
    10321 caaatataca cactttttct aaataatgaa atagctttca caaaaaagta gtctgagtct
    10381 cctttcaaca ttcagatttg tttagttatc aactccactc tactacagta agccaattca
    10441 accattc
//
LOCUS       DAORKP010000076        10444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_771_length_10444_cov_8.821446, whole genome shotgun sequence.
ACCESSION   DAORKP010000076 DAORKP010000000
VERSION     DAORKP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_10444_cov_8.821446"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..626)
                     /locus_tag="PLN75_05610"
     CDS             complement(105..626)
                     /locus_tag="PLN75_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRG03625.1"
                     /translation="MAYIKSVRGFTPQFGENCFLAENATVVGDVIMGDGCSVWFNAVL
                     RGDVNAIRIGNNVNIQDGTVLHTLYQKSVVEIGDYVSVGHNVIIHGAKINDYALIGMG
                     ATLLDYAEVGEGAIVAAGSVVLSNTKIPPYTLWAGVPAKYVKDVEPHQTNEMNRKIAH
                     NYAMYSGWYKEDE"
     gene            complement(759..1199)
                     /gene="ssb"
                     /locus_tag="PLN75_05615"
     CDS             complement(759..1199)
                     /gene="ssb"
                     /locus_tag="PLN75_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRG03626.1"
                     /translation="MSINKAILVGNVGRDPEVRYLDKNVAVANFTMATTERGYTMQNG
                     NQVPDRTEWHNVVAWRGLAELAEKYIRKGTQLYVEGKIQTRSWEKDGVKRYTTEIMAE
                     TIQLLGKRPEQNETSPVAPIATAENEALKASDIPLANETDDLPF"
     gene            1405..2214
                     /gene="nagB"
                     /locus_tag="PLN75_05620"
     CDS             1405..2214
                     /gene="nagB"
                     /locus_tag="PLN75_05620"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRG03627.1"
                     /translation="MRLIIQPDYQNVSKWAANYIASRINAANSTKDKPFVLGLPTGSS
                     PLGTYKYLINLYKQGTLSFKNVITFNMDEYVGLKQDHEQSYYTFMWSNFFSHIDILPE
                     NVHILNGNAADLEAECAAYENAMKEVGGIDLFLGGIGPDGHIAFNEPASSLQSRTRQK
                     TLTTDTMIANSRFFDNDVNKVPKTALTVGVGTVLDAREVVIIVNGHNKARALRHAVEE
                     PINHMWTISALQMHPKGIIVCDYDACAELKVGTYKYFLDIEKDKLDPDTLI"
     gene            2376..3902
                     /gene="atpD"
                     /locus_tag="PLN75_05625"
     CDS             2376..3902
                     /gene="atpD"
                     /locus_tag="PLN75_05625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRG03628.1"
                     /translation="MSEIVAGRISQIIGPVVDVFFDLQKKDEEKLPAIHDALYIDRGD
                     GRQLVVEVQQHIGENTVRTVAMDSTDGLRRGLIVKTLGSPITMPVGNQVKGRLMNVIG
                     ESIDGMKSLDREGAYPIHREPPKFEDLSTSTEVLFTGIKVIDLIEPYAKGGKIGLFGG
                     AGVGKTVLIQELINNIAKGHSGFSVFAGVGERTREGNDLLREMIESGVIRYGEEFKKS
                     MDEGNWDLSKVDYDEVEKSQATLVFGQMNEPPGARSSVALSGLTVAESFRDGVGENKK
                     GNDILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGTMQERITSTKQGSIT
                     SVQAVYVPADDLTDPAPATTFSHLDATTVLSRKIAELGIFPAVDPLDSTSRILNPEVL
                     GEDHYNTAQRVKQILQRNKELQDIISILGMEELSDEDKITVNRARKIQRFLSQPMYMA
                     AQFTGLPGVMVPIEETIRGFKMILDGEVDEYPEMAFLNVGVIDEAIEKGKKLLEQAKK
                     "
     gene            3912..4160
                     /gene="atpC"
                     /locus_tag="PLN75_05630"
     CDS             3912..4160
                     /gene="atpC"
                     /locus_tag="PLN75_05630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRG03629.1"
                     /translation="MKLEIVTPDQIVFSGEVSLVTVPGTSGRFTILENHAPIISSLSA
                     GNITWRVKNTDTSILASDGFVEVSKNVVSIAVEGITNE"
     gene            4153..4536
                     /locus_tag="PLN75_05635"
     CDS             4153..4536
                     /locus_tag="PLN75_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03630.1"
                     /translation="MNKAFHLTWILAASIAALTIVAYVVFYFTGFSFMPEYFYITPVF
                     FLMLTLVLAFYVKKHLKKEKELSVGGILGIRVLLLAPVVIVLVINMLIDKEHILPLTV
                     AYILYDLVFSVFETKILLALNNNKK"
     gene            4592..5638
                     /gene="atpB"
                     /locus_tag="PLN75_05640"
     CDS             4592..5638
                     /gene="atpB"
                     /locus_tag="PLN75_05640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRG03631.1"
                     /translation="MNISPLRASGGEAEKEKPFDIKEMIFSHVLDSYEWHILTIGEKH
                     ISIPLLMIVKDERGWHAFSTKKLAHGHEHNGFYISQTDPYKGKIVTKNAAGEIIRPLD
                     LSLTKNAASILVASIILIVILLSIASQYKKNPLRSMKGFAGAIEQLTMSVHNDIIKPS
                     IPNDYKRFAPYLLTVFYFIFFNNLFGLIPLFPGGANVTGNIAVTFVLAMLTFVATNFF
                     GNKEYWKEIFWPDVPVFLKTPVFPLMQLIEFIGILTKPFALMIRLFANMLAGHMIVLV
                     LMGLIFIFAAMLGVGAAAGVSVVSVAFSIFMVLLDVLVSFIQAYVFTMLSSIFIGMAR
                     AEHHTHKVHESTEH"
     gene            5691..5948
                     /gene="atpE"
                     /locus_tag="PLN75_05645"
     CDS             5691..5948
                     /gene="atpE"
                     /locus_tag="PLN75_05645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRG03632.1"
                     /translation="MLLTILLQVAASAGFANLGAALGAGIAVIGAGLGIGRIGGQAMD
                     AISRQPEAAGDIRSNMIVIAALIEGVAFLAVIVCALAIFIK"
     gene            5952..6452
                     /gene="atpF"
                     /locus_tag="PLN75_05650"
     CDS             5952..6452
                     /gene="atpF"
                     /locus_tag="PLN75_05650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRG03633.1"
                     /translation="MELFTPEVGLIFWMLIPFLVVFFILAKFAWPAILKGVNARNKYI
                     DDSLLAAKQARDELARVKADGQAIVEEARKEQSRILAEAAKSRDLIVNGAKDKATEEA
                     SKIMENAREQIALEKEDAIRQIRREVATLSVEISEKVLRKNLEKKNEQMDMIERLLDE
                     FNIPKS"
     gene            6541..7095
                     /locus_tag="PLN75_05655"
     CDS             6541..7095
                     /locus_tag="PLN75_05655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRG03634.1"
                     /translation="MNTGLISVRYATALFDFAVETKSVERVYGEAKMLTHMFLKMGEL
                     RIVLDNPVLPQSEKRQLILNAAGGSTSKTFEHFTDLLLKNGRENQVQYIMLKYIDLYR
                     KDKNILYGKLTTAFEVDKATEERLISLVTDKTGGTLELEKVVDPEILGGFMLEVEDVR
                     WDASLSGQLTRIKNEYIERNRRIV"
     gene            7154..8737
                     /gene="atpA"
                     /locus_tag="PLN75_05660"
     CDS             7154..8737
                     /gene="atpA"
                     /locus_tag="PLN75_05660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRG03635.1"
                     /translation="MSDTKIKVSEVSEVLRLQLEGIDNGVKFDEIGTVLQVSDGVVRI
                     YGLRNAEANELLEFDNGIQAIVMNLEEDNVGAVLLGPSNEIKEGYSVKRTKRVASIFV
                     GEGMLGRVINPLGEGIDGRGKITGELLEMPLERKAPGVIYRQPVNQPLQTGLKAIDAM
                     IPIGRGQRELIIGDRQTGKTSIAIDTIINQRRNFDEGDPVYCIYVAIGQKGSTVASIV
                     NTLKEHNAMEYTIVVAANASDPAALQYYAPFAGAAIGEYFRDTGRHALVIYDDLSKQA
                     VSYREVSLILRRPSGREAYPGDIFYLHSRLLERAAKIINQEEVAKQMNDLPESLKGRV
                     KGGGSLTALPMIETQAGDVSAYIPTNVISITDGQIFLDTDLFNQGNRPAVNVGISVSR
                     VGGNAQIKAMKKVAGTLKIDQAQFRELEAFTKFGGDMDAVTAMTIDKGQKNTKLLVQK
                     LHQPMAVEKQVAVLYCGTHGLLSKVPLEKVTVFEEEFLSTLELTHQEDVLDVIKKGII
                     DEKVTTLLEKVAEEVARRI"
     gene            8821..9690
                     /locus_tag="PLN75_05665"
     CDS             8821..9690
                     /locus_tag="PLN75_05665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRG03636.1"
                     /translation="MASLKEIKSRIQSVKSTQKITSAMKMVSSAKLRKAQKRIESFYP
                     YEQKLNQILNNFLNAEEESSSIYAESREIKRVAVIAFSSNSSLCGGFNSNIAKKLTAT
                     LERYNHLGRENILIFPIGKKIGRVVKKIGFPIENFDEMADKPTYPAAQALTDRVMQLF
                     RNGEVDKVELIYHHFKNKSSQVLLEETLLPITLESAHNYQSGVLLNYLVEPDRKTIMS
                     ELIPKVLRLKVYTALLDSNASEHAARTIAMQIATDNADDLLQELNLQYNKSRQQAITN
                     ELLDIIGGSFGQS"
     gene            complement(9767..>10444)
                     /locus_tag="PLN75_05670"
     CDS             complement(9767..>10444)
                     /locus_tag="PLN75_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRG03637.1"
                     /translation="PAGKNLAETANVSTAGEVPISIIRDAKGKVEILASRNQQDIVYT
                     LNKAKKESSYFMPLQLRDGGTVTAWFKGNPNTKTTAVFDKILTVQLEVVSASSVEPGS
                     GDAAHLVDGDPTTIWHTMYSVTVAKHPHWVDFDMGNEKTLKGFTYLPRQNGTNGNVKD
                     YTLQVSTDGKTWSEPVAKGKFDRSGNEKIVMLDKSVKARYVRFTALSEVTGADFASGA
                     EFGVLAE"
BASE COUNT         3137 a         2262 c         2240 g         2805 t
ORIGIN      
        1 atttaaagct tctcctgttt tgttaaataa atataaaaaa aaaagaaacg cttcgtcttt
       61 atttacggcg aatcgttttt cctttttagt tctgtttaca atatttattc atcttccttg
      121 taccaacccg aatacatggc atagttgtga gctattttgc ggttcatttc attggtttga
      181 tgaggttcca catcctttac gtattttgcc ggcacacccg cccaaagcgt ataaggaggt
      241 atttttgtat tgctcaacac caccgaaccg gctgcaacga tggctccctc gcccacttct
      301 gcatagtcca gcagcgtagc acccatacct atgagcgcat agtcgttgat ttttgctccg
      361 tgaatgataa cattatgccc taccgataca tagtcgccta tttcgacaac cgacttctgg
      421 tacagcgtgt gcagtacggt tccgtcctgt atattcacat tgtttccaat ccggatagca
      481 tttacatcgc cacggagcac agcattgaac cacacgctgc aaccatctcc cattattacg
      541 tcgccaacta ccgtggcatt ttctgccagg aaacaatttt cgccaaattg cggtgtaaaa
      601 ccgcgtacag attttatata agccatttct ttattgaatt attaatttta cgagtattaa
      661 acaaaaaaaa tccattcaat gttttcattt ttacataata gaacaggaca cacagctatt
      721 caccgtgtgt cctgtttttt tcgtaatcac tgtctttttt agaaaggcaa atcgtctgtt
      781 tcattcgcaa gtggaatatc actggctttc agggcttcgt tttcagctgt tgcaattggt
      841 gcgacaggtg aagtttcgtt ttgttccggg cgtttaccaa gcagttggat ggtttcggcc
      901 atgatttcag ttgtataacg ttttactcca tctttttccc aactgcgagt ctggatttta
      961 ccttccacat acaattgagt acctttccga atgtattttt cggcaagttc ggccaaacca
     1021 cgccatgcta ctacattgtg ccattctgtg cggtcaggca cctgattccc attttgcatg
     1081 gtataaccac gttcggtagt agccattgta aaattagcta ctgcaacatt cttgtctaaa
     1141 taacgtactt cagggtcacg acccacattt cctactaaaa ttgctttgtt gattgacata
     1201 ataaataaaa tttgataatt aatataaaac aattgcacgg caaatataca agtttttttt
     1261 tactttcaat atttttatcg tatttttttc aaaacatttt tttcagcaaa aaaatttgtt
     1321 gtatttttaa acttcaattt tttacagttt tagaaaataa aaccgtaaat ttgtacttta
     1381 ttcatatata aaccaaaaaa aatcatgaga cttatcattc agcctgatta tcagaatgtt
     1441 tcaaaatggg ctgcgaatta catcgcatcc agaataaatg cggccaattc caccaaagac
     1501 aaaccttttg tactcggctt acctacggga tcttcgccgc ttggcaccta caaatacctt
     1561 attaatctct acaagcaagg caccctgtca ttcaaaaacg taatcacgtt caacatggat
     1621 gaatatgtag gactaaaaca ggaccacgaa cagagctact acacgttcat gtggtccaac
     1681 tttttcagcc acattgatat cctacccgaa aatgtacaca tactgaacgg taatgccgcc
     1741 gacctggaag ctgaatgtgc tgcctacgaa aacgcgatga aggaagtggg tggcatagat
     1801 ttatttctgg gaggaattgg gcccgacgga cacatcgcgt tcaacgaacc tgcttcttct
     1861 ttgcagtcgc gtacacggca gaaaacgctt accaccgata cgatgatagc caactcgcgc
     1921 ttctttgaca acgacgtgaa taaagttccg aaaacagcac ttaccgtagg tgtaggcacc
     1981 gtactggatg cccgcgaagt agtaatcata gtgaacggac acaacaaagc gcgggccctt
     2041 cgtcatgctg tggaagaacc catcaaccac atgtggacca tcagcgcact gcaaatgcac
     2101 cctaaaggta ttatagtatg cgattacgac gcctgcgccg aactgaaagt aggcacttac
     2161 aaatacttcc tggacataga aaaagacaaa ctcgatccgg atacactgat ataaaagtca
     2221 gattaattta tccgaaccgc cctaccggtg gttgggcggt tttgttttta aaaaagtaac
     2281 gaaaaaggga taaaaaacca tagggaaaat aattgtcact ctaaaagttt ttttgtacgt
     2341 ttgtatattc aaaaaataaa aaacacctga aaaatatgtc agaaatagta gcaggtcgca
     2401 tttcacaaat tatcggtccg gtagtggatg tgtttttcga tttacagaaa aaagatgaag
     2461 aaaaacttcc ggccattcac gatgcacttt atattgaccg cggcgatggg cgccagctgg
     2521 tagtagaagt tcagcaacac attggcgaaa acacagttcg cactgttgca atggattcta
     2581 ccgacggatt aaggcgcgga ctaatcgtaa aaacgcttgg aagccccatt acaatgcctg
     2641 tgggaaatca ggtaaaagga cgcctgatga atgtaatcgg cgaatcaatc gatggtatga
     2701 aatccctcga tcgcgaaggc gcatatccga tacaccgcga gcctccgaaa tttgaagacc
     2761 tttccacctc aaccgaagta ctttttaccg gaatcaaagt cattgacttg atagaaccct
     2821 atgcaaaagg gggtaaaatc ggcttattcg gtggtgccgg agtaggtaaa accgtactta
     2881 tccaggaact aatcaacaac attgcaaaag gacacagtgg tttctcggtg tttgccggtg
     2941 tgggtgagcg tacgcgcgaa ggcaatgacc tcctgcgcga aatgattgaa tcgggtgtaa
     3001 tccgttacgg tgaagaattc aaaaaaagca tggatgaagg caactgggat ttatccaagg
     3061 tagattacga tgaagtggag aaatcgcagg ccacactcgt cttcgggcaa atgaatgaac
     3121 ctcccggtgc acgttcatca gttgctttat cgggtttaac ggtagccgaa tcattccgcg
     3181 acggtgtggg tgaaaacaaa aaaggaaatg atatcctgtt ttttatcgac aatattttcc
     3241 gttttacaca agcaggctcc gaagtgtcgg ctttgctcgg acggatgcct tcggcggtag
     3301 gatatcaacc tacactttcc actgaaatgg gaaccatgca ggaacgaatc acctccacaa
     3361 aacaaggctc aatcacctcg gttcaagctg tttatgtgcc tgccgacgac cttaccgacc
     3421 cggcaccggc caccacattc tcacacctgg atgctactac agtattaagc cgtaagattg
     3481 ccgaactggg tattttccca gctgtggatc cgctggattc aacctcacgc atcctgaatc
     3541 cggaagtttt aggagaagat cattacaata cagctcagcg ggtaaaacag attctacaac
     3601 gtaacaaaga gcttcaggac atcatttcga ttctcggtat ggaggagctt tctgacgaag
     3661 acaagataac cgttaaccgg gcccgtaaaa ttcagcgttt cctgtctcaa cctatgtata
     3721 tggctgcaca gtttaccgga cttcccggag ttatggtgcc cattgaagaa acgatacgcg
     3781 gattcaaaat gattctggac ggagaagtag atgaataccc tgaaatggca ttcctaaacg
     3841 taggcgtaat agatgaagcg atagaaaaag gcaagaaact gctggagcag gcgaaaaaat
     3901 aaaagctgaa aatgaaactt gaaatcgtaa caccggatca aattgtattt tcgggagagg
     3961 tcagtctggt taccgttccg ggaacgagcg ggcggttcac catacttgaa aatcacgcac
     4021 ccattatttc gagcctctcg gcaggcaaca ttacctggcg cgtaaaaaac acggatacga
     4081 gcatcttggc ttcggatgga tttgtagaag taagtaaaaa tgtggtatcc attgccgttg
     4141 aaggaattac gaatgaataa agcatttcat ttaacatgga tactggctgc aagtattgca
     4201 gctttgacca tcgtagctta tgtagttttt tatttcacag gtttcagctt tatgcctgag
     4261 tatttttaca tcacgccggt attctttctt atgctgacgc tggttttagc tttttatgta
     4321 aaaaaacacc tgaaaaagga aaaagagttg tcggtcggtg gtatattggg catcagagtt
     4381 ctcctgctgg ctccggttgt aattgtgctg gtgataaaca tgctcattga caaagaacat
     4441 attctgccgc ttacggtggc ttatatactt tacgatctgg tattttctgt gttcgaaaca
     4501 aagattttac tggcgttaaa caataataaa aaataatatg ccctcttatt ttaaacgatt
     4561 ttcgctactc ctgcttacat tcatttttat tatgaatata agccctttaa gagcatccgg
     4621 cggcgaagct gagaaagaaa aaccgttcga tatcaaagaa atgattttct ctcacgtgct
     4681 ggattcatac gagtggcaca ttctgactat cggtgagaaa catatttcca ttccgctgct
     4741 gatgattgtg aaagacgaga gaggatggca tgcattctca actaaaaaac tggctcacgg
     4801 acacgaacat aacggttttt acatttctca gacagatcct tacaaaggaa aaatcgtaac
     4861 taaaaatgca gcaggagaaa ttattcgtcc gttggattta tcactaacca aaaacgccgc
     4921 atcaatcctc gtagcaagta ttattctgat tgtcatacta ctcagcatcg cttcacaata
     4981 caaaaaaaat ccgctcagat ccatgaaagg atttgcgggc gccatagagc aactcacgat
     5041 gagtgtacac aacgatatca tcaaacccag tattccgaat gattacaagc gatttgcacc
     5101 ctatctgctc accgtttttt attttatctt cttcaataat ctgttcgggc tgataccgct
     5161 gtttcccgga ggtgccaatg tcaccggtaa tattgccgtc acatttgtgc tggcaatgct
     5221 tacgtttgtt gccaccaact tttttggcaa caaagaatac tggaaagaga ttttctggcc
     5281 ggatgtgccg gtttttctga aaacacctgt tttcccactc atgcagttaa tcgaatttat
     5341 tggaatactt acgaaaccct ttgccttgat gattcgtctt tttgccaata tgctggccgg
     5401 acacatgatt gtgctggtac ttatgggatt gatatttatt tttgcggcaa tgcttggtgt
     5461 aggagctgcc gcaggcgttt cagtagtttc agttgcattt tccattttta tggtgctgct
     5521 tgatgtgctt gtttctttca tccaggcgta tgttttcacc atgctttcat ccatatttat
     5581 aggcatggca agagccgaac atcacactca caaagttcat gaatcaaccg aacactaaca
     5641 atcaataatt aaattacata aatattacaa acaattaaaa gaaagacatt atgttactaa
     5701 ccatcttatt acaagttgcc gcatcagcgg gtttcgcaaa tttaggcgca gcactgggcg
     5761 caggtattgc agtaattggc gcaggtcttg gcatcggccg cattggcgga caagctatgg
     5821 atgctatttc acgtcagccg gaagcagccg gcgacatacg ttcgaatatg atcgtgattg
     5881 ctgcacttat cgagggggtt gcattccttg cagttatcgt ttgtgctctg gccattttca
     5941 tcaaatagca tatggaactt ttcactcctg aagtcggtct gatcttctgg atgctcattc
     6001 cttttctggt tgtctttttt attctggcta aatttgcatg gcctgcaata ctgaaaggcg
     6061 taaacgcaag gaataagtat atagatgatt cgcttctcgc tgccaaacaa gcccgcgacg
     6121 agctggccag agtgaaggcc gacggacaag ccatagtgga agaagcccgc aaagagcaat
     6181 ccaggatact tgcagaagct gccaaatcaa gggatttgat agtaaacggc gccaaagaca
     6241 aagctaccga ggaggcttcg aaaatcatgg aaaatgcccg cgaacaaatt gcacttgaga
     6301 aagaagacgc cattcgtcaa atccggagag aagtagcaac actttcggtt gaaatttcag
     6361 aaaaagtgct gcgaaagaat cttgaaaaga aaaacgaaca aatggatatg attgaacgtc
     6421 tgctcgatga attcaacata ccgaaatcct gatttggatg aatagtataa agaaatgtat
     6481 cattcacata tacatttatt catataccat tcaatctgat tatacttata aattaaaagt
     6541 atgaataccg gacttatatc agttagatat gccaccgccc tgtttgattt tgcagtagaa
     6601 acaaaatcag tggaaagagt gtatggcgaa gccaaaatgc tgacccacat gtttttgaaa
     6661 atgggtgaac tgaggattgt gctcgataat ccggtattgc cacaatcgga aaaacgacaa
     6721 ctgattctca atgcggccgg cggatcaacc agcaaaactt tcgagcattt caccgatttg
     6781 ctcctgaaaa acggacgaga aaatcaggtg caatacatca tgctgaaata catcgatctg
     6841 taccggaaag acaaaaacat cctttacgga aaactcacta cagctttcga ggtggacaaa
     6901 gccaccgaag aacgtttgat ttcgctggta accgataaaa cggggggcac gcttgaactg
     6961 gaaaaagtag tagatcctga aatactgggc ggattcatgc tcgaagtgga agatgtacga
     7021 tgggatgcaa gtctctcggg acagttgaca cgcataaaaa atgagtacat tgagcgcaac
     7081 cgaagaattg tttgatatac atttcaatca tttcaaacta atatcgtaaa taacaaaaag
     7141 aaaaaaaata cagatgtctg atacgaaaat aaaagtgagc gaagtctcag aagtgttgcg
     7201 actgcaactc gaaggcattg acaacggagt aaaatttgat gaaattggta ccgtattgca
     7261 ggtaagcgat ggagtggttc gaatctacgg attgcgaaat gctgaagcca atgagctgct
     7321 tgaatttgac aatggcattc aggccatcgt tatgaacctc gaagaagaca acgtaggagc
     7381 cgtattgctg ggaccatcca atgaaataaa agagggatac agtgtaaaac gaaccaagcg
     7441 ggtagcttca atttttgtgg gcgaaggaat gctcggacgt gtcatcaatc cgctgggaga
     7501 aggtattgac ggacggggaa aaattaccgg cgaactactc gaaatgcctc ttgagcgcaa
     7561 agctccgggt gtaatctatc gtcaaccggt gaatcagccg cttcaaaccg gtctgaaagc
     7621 catagatgca atgattccaa tcgggcgcgg gcagcgggaa cttatcatcg gtgaccgcca
     7681 gaccggaaaa accagtattg ccattgatac aattatcaat cagcgacgaa actttgatga
     7741 aggtgacccc gtttactgta tttacgttgc aatcggtcag aaaggatcca cagttgcatc
     7801 cattgtgaac accctgaagg agcacaatgc catggaatat acgattgtag tggcagcaaa
     7861 cgcctccgat ccggccgcct tgcagtatta tgctccgttt gccggcgctg ccatcggcga
     7921 atacttccgc gataccggac gccatgcgct ggttatttac gacgaccttt caaaacaagc
     7981 cgtttcgtac cgcgaagttt cgcttattct tcgtcgccct tccggacgcg aagcctatcc
     8041 gggcgatatt ttctatctgc actcgcgcct gctcgaaaga gccgctaaaa tcatcaatca
     8101 ggaagaagta gccaaacaaa tgaacgacct gcccgagagc ctcaaaggca gagtgaaagg
     8161 cggtggttca ctcacagctc ttccgatgat agaaacccag gcaggcgacg tatctgccta
     8221 tattccaacg aacgtaatct cgattaccga cggacaaata tttcttgata ccgacttatt
     8281 caatcaggga aatcgtccgg ctgtaaatgt gggaatctcc gtatcgcgtg taggcggtaa
     8341 cgctcagatc aaagccatga aaaaggtagc cggaacacta aaaattgacc aggcacagtt
     8401 tcgtgaactt gaagcattta ccaagtttgg aggcgatatg gatgcggtga ctgccatgac
     8461 cattgataaa ggacagaaaa acacaaaatt gctggttcag aaacttcatc aaccaatggc
     8521 tgtagaaaaa caagtggcag tactctattg cggtactcat gggctgcttt ctaaagttcc
     8581 acttgaaaaa gtaactgttt ttgaggagga atttctgagt acccttgaac tgacacatca
     8641 ggaagacgta ttggacgtaa tcaaaaaggg aataatcgac gaaaaagtga caactctgct
     8701 tgaaaaagtg gcagaagagg ttgcaagaag aatataggaa tagctctgac ttatttaaca
     8761 gaaaattttt agtaattaaa agtctgatag actttggtct attagtctaa agtccgacga
     8821 atggcttcat taaaagaaat aaaatcgagg atacaatccg taaaatctac gcagaaaatc
     8881 acttcggcga tgaagatggt ttcgtctgcc aagttacgca aagcgcagaa aaggattgaa
     8941 agtttttatc cgtacgagca gaaactaaat cagattctga acaatttcct gaatgcagaa
     9001 gaagaaagtt cttccatcta cgctgaaagc cgggaaatca agcgggtggc agtcattgca
     9061 ttttcatcta actcaagtct ttgcggagga ttcaactcaa acattgcaaa aaaactaact
     9121 gccacactcg agcgatataa tcatctcggg cgggagaata tcctgatttt cccgatagga
     9181 aaaaaaattg gcagagtggt aaaaaaaatt ggatttccta ttgaaaattt cgatgaaatg
     9241 gccgataaac caacctatcc ggccgcacag gcactgactg acagagtgat gcaactcttt
     9301 agaaacggtg aggtggacaa agtggaatta atctatcatc atttcaaaaa caaaagttca
     9361 caggtgctac ttgaagaaac cctgctgccc atcacactgg aatctgctca taattaccag
     9421 agcggtgtat tgctaaatta cctggttgaa cccgacagaa aaacgattat gagcgaatta
     9481 ataccgaaag tactgcgtct gaaagtgtac accgctcttt tggacagcaa cgcatctgaa
     9541 cacgctgccc gaaccattgc tatgcagata gccaccgaca atgctgatga cctgctacag
     9601 gagctcaatc tgcaatacaa caaatcgcgt cagcaagcta taaccaacga actgctggat
     9661 attatcggag gctctttcgg acagagttaa agaattgttt gatacataaa aaaaaaccag
     9721 ctaattctca acaaattagc tggttttttt atataagtgg aattcgttat tccgccaaca
     9781 ctccgaactc tgctccactg gcaaagtcgg cgccggttac ttcactcagg gctgtaaaac
     9841 gcacatagcg agcttttaca gatttgtcga gcattactat tttttcgtta ccggaacggt
     9901 caaatttacc tttagcaacc ggttccgacc aggttttccc gtcggtgctc acttgcagcg
     9961 tgtaatcttt cacgtttccg ttggttccat tctggcgtgg caggtaagtg aagcctttca
    10021 gcgttttttc attgcccata tcaaaatcca cccaatgcgg atgttttgcc acagtaaccg
    10081 aatacatggt gtgccaaata gtagtaggat cgccatctac caggtgagca gcatcgcctg
    10141 aaccgggctc cacactactt gcagaaacca cttccagctg cacggttaag attttatcaa
    10201 atactgccgt tgtttttgta ttcggattgc ctttgaacca ggcagttacc gttccgccat
    10261 cacgcagttg cagcggcata aagtacgaac tttctttttt ggctttattt aatgtgtaaa
    10321 caatatcttg ctgattgcgg cttgccaaga tttccacttt tcctttggca tcgcgaatga
    10381 tggaaatcgg cacttcaccg gctgtggaaa cgttggctgt ttcggccaaa tttttgcccg
    10441 cagg
//
LOCUS       DAORKP010000077        10431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_774_length_10431_cov_9.742965, whole genome shotgun sequence.
ACCESSION   DAORKP010000077 DAORKP010000000
VERSION     DAORKP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_10431_cov_9.742965"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1293
                     /locus_tag="PLN75_05675"
     CDS             181..1293
                     /locus_tag="PLN75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRG03638.1"
                     /translation="MKYGFFFVFFTFLLSIIAYTTIRGYQTLAPYGNFKTVYLVSMVV
                     LSVVMFAGIGLENQLPHGLAKGLSFVGHTFFIVAAYLFISFLLTDIFRAVNHFVPLVT
                     NIQAFRLGAMVGTLAILTVLLVWGNYKFNHPQKVQLTIHSNKSHKKNREIKIVAVSDI
                     HLGTSIDKKRLRQYVDLINAEKPDLVLIAGDLIDRSIKPVISQKMDEELRTIQAPMGV
                     YVIFGNHEYYGEGDHHIADFYRKSNITLLRDTSVVINNELLVVGRDDKHNPNRRHLKE
                     LFPPDTDKPVIVLDHQPYHLQEAESSRVDLQISGHTHNGQFFPANLIVKSMFELGYGH
                     LKKGNTHFYVSSGLGLWGPQYRLGSQSELVVVNFRF"
     gene            complement(1267..3753)
                     /locus_tag="PLN75_05680"
     CDS             complement(1267..3753)
                     /locus_tag="PLN75_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRG03639.1"
                     /translation="MKKILSLFTVFIISFSVVTAQSWIRVNQIGYLEDDVKVAVWISK
                     DSRLITEFDLVDVASGSIVYSSDKVKQTGPQPAFNTSARLDFSGFNLPGSYKIRVNET
                     ESSVFRIGNDIYAGAADIPLQYMRQQRCGYNPFLKDSCHTTDGISVGDPEGKRDGKYY
                     NTTGGWHDASDYLQYVTTSANAVYQMLFAYTQHPESFGDNFDANGDPGSNGIPDILDE
                     AKWGLDWLVKMNPDSLTYFNQLADDRDHVGMTIPSEQKVDYGWGAGNARPVYFCSPSP
                     QGLRQYKNRSTGLASTLGKYSSSFALGSRVLAKYYPEFSKNIALKAAHAYRKGMTNPG
                     VCQTACTVSPYFYEEDNWADDMQLAAAEMYLLTKSSENLNQAVNYGRMEPVTPWMGAD
                     SARHYQWYPFVNLGHYHLAKQNANSRISREFIRNMRSGLQRVKDRAADNAFMNGVPFI
                     WCSNNLTIGLITQCRLYHELTGDDTFVEMETSMRDWLFGVNPWGTCMIVDYPANGDTP
                     SDPHSAFTKLRNYPISGGIVDGPIYAPIFNSLRGVHLSKEDMYAPFQSDYVVYHDDFA
                     DYATNEPTMDGTASLTFYLSYLNSKAIARKVVQGGIVRGNPLERKLALVFTGHEFADG
                     AATISKTLKKNGVKASFFLTGDFYRKFPQVTKQLQQEGHYMAPHSDKHLLYADWENRD
                     LTLVTSYSFEKDLQNNYRAMEKAGLKIDEPRYFIPPYEWYNSEISDWAKQMNVQVVNF
                     TPGTGSNADYTTPEMNSYKSSEVIYNNILQFEKENTLNGNLLLLHIGTHATRTDKFYY
                     RLDDLIKELKTRGYEVVRVDELLKPEIHHY"
     gene            complement(3817..4230)
                     /locus_tag="PLN75_05685"
     CDS             complement(3817..4230)
                     /locus_tag="PLN75_05685"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRG03640.1"
                     /translation="MRQALDEARQAFRRDEVPVGAVIVCNDRIIARGHNLTETLIDVT
                     AHAEMQAITAAANFLGGKYLTDCTLYVTVEPCVMCAGALGWSQISRIVYGASDEKRGF
                     TRFAPDAIHPKTEVVAGVLEEECSALMKEFFKKKR"
     gene            complement(4428..6311)
                     /locus_tag="PLN75_05690"
     CDS             complement(4428..6311)
                     /locus_tag="PLN75_05690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRG03641.1"
                     /translation="MEDLKNLEKIDVVYGDGNIITLEGLEHIRRRPGMYIGKLGDGSH
                     ADDGIYVLLKEVIDNSIDEFRMGHGKTIEIRIENERVSVRDYGRGIPLNSVVKAVSIM
                     NTGGKFDSQVFKKSVGLNGVGTKAVNALSSSFMVESYREGKSKKATFSEGKLVEEQDF
                     EGLKKENGTLTEFIPDATLFPNYEYRDEFVETMLRNYAYLNTGLVLNYNGKKISSKNG
                     LIDLLNENMTSESLYPIVHLRGEDIEIAFTHSNQYGEEYYSFVNGQHTTQGGTHQSAF
                     REAVARTIKEFYNKNMEITDIRNGIVAAVAIGVEEPVFESQTKTKLGSRDIHKNGITV
                     NKFISDFVKKELDNYLHRNGETADILLQKIQESEKERKAIAGVTKQARERAKKVSLHN
                     RKLRDCRYHFNDAPVKDEAKNKLMEDSSIFITEGDSASGSITKSRDVNTQAVFSLRGK
                     PLNSFGLTKRVVYENEEFNLLQAALNIEDGIEGLRYNKVIVATDADVDGMHIRLLIIT
                     FFLQFFPDLIKKGHVYILQTPLFRVRNKKETRYCYSEEERVDAIQTLGPNPEITRFKG
                     LGEISPDEFRNFIGADIRLEQVKLRREDAVQALLSFYMGKNTSERQGFIIENLVVEED
                     TAP"
     gene            complement(6322..7743)
                     /locus_tag="PLN75_05695"
     CDS             complement(6322..7743)
                     /locus_tag="PLN75_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRG03642.1"
                     /translation="MNKKITYLITIISLILSCNFGVRAQSMETLKKQRMEIQERINET
                     NKLLKETQKNEKASLKKLNALKKNLNERRNLIKNYNDEINVLDRKMNSLTAERIELEK
                     QLEKLKGDYARLIRKTQANMNSYSRLMFLLSAKDFDQTIRRVRYLREFTDYKKEQVKQ
                     IEQVKTQIALKTDSLDQNKLAKMGALKAKQLEAEKLKKDESEEKSMYASLQQEEKRLS
                     EEYRAHQRKKDQIDNKIQQVIEEEIRRAEARRRAEEARIRAEQEARRKIEERKMAEAR
                     AAEQRKKAESQNKKTETIAEAKPAETTKPVETTSKTEVATTTRSGNAESVSAYTREER
                     LLSGNFANNQGRLPWPVDRGYIRGHYGTQPHPELKYVTINNKGVYFRSPAGTNARAVF
                     EGVVTRIFSLPGTGNAVIVQHGNYRTLYANLSEVFVRSGEQVSARQPIGKIYTDDESG
                     QAELQFNLYNGSQMVNPESWLTR"
     gene            complement(7750..8586)
                     /locus_tag="PLN75_05700"
     CDS             complement(7750..8586)
                     /locus_tag="PLN75_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRG03643.1"
                     /translation="MKPKLIRILVVAAAIVLFGACKTTKTAVKPTQTATTPTEAAHEF
                     TPADMVKQLQLAQPAFRTANASKMSVYVDFKGRQMDVKASCKIVSDSALYLSIQPFMG
                     VELFRLEMTPSTLIVVDKMNKRFYESNYSIFRNTLGVTVNYDVIQSLISNRLFVPGDK
                     VFLPDEFNWKDNTPGKSLTLQTESFNQEVLVDVLLSRIAAVMILSADSTYTMKTDYSD
                     FKKTDGMLFPQKITIDANKGEEKSSFHFTIEQVEFNKPVVMEPTNLSRYTRGNINSLF
                     RK"
     gene            complement(8583..10325)
                     /locus_tag="PLN75_05705"
     CDS             complement(8583..10325)
                     /locus_tag="PLN75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03644.1"
                     /translation="MGRFRGAFLLILLLTIPAFALPKGKMSVPVAVRLSADQQARFDY
                     YFFEAQRLKNIQEYDAQIDALRMCLEIDSTSAAAQSETGYAYARLNWPTESMRAFRKS
                     VEAAPENWWYRTQYISMLSTQKLYPTAIEQAEELRKRFPQREEVYTMLSSLYKQTGEF
                     DKAIGALNQLEVFTGINEYLSIEKFQLYALLKKDNKAIEEILKLTQKYPQESRYRVLL
                     GDIYMDQKQPRKAYEIYQQILRDDPGNPFVYVSLSNYYKLNRQDDKALESIVSALKNP
                     ELPSDTKMDILGQYVDQLLGSNQKIGETENLFKILIDMYPLDEMAHAYYALFLQHQKR
                     TAEALEELESVININPKNEAAWKNSLQILTEKNDTTGVLSLTERALHELPEVPEFYFY
                     RSIALYQQQKYEEALQTNFTAIENLTSAAPPVLSSFYGQIGDIYFKMDDKEHAFENYE
                     KALEANPANVYIMNNYAYYLSVEGTDLRKAERMSAKTVELEPNNSTYLDTYAWIFYKQ
                     GNYNLARIYIDKAVSNMKEDEISEVILEHSGDIYYALKNTEKALEMWQKALKLNENNA
                     ELIKKTDQAKNEIK"
BASE COUNT         2382 a         2365 c         2495 g         3189 t
ORIGIN      
        1 acatagaata atttatactt attctcatat attcggaaac atagtgaaaa atatcttcga
       61 tatttttaaa ctattgttat cgaaattaaa taaaacagca cgaaaattca ataaaataag
      121 actattgttc tctatgtttt aaaagtcgag acgagtttaa tatatttaat ttaactaatt
      181 atgaaatacg gatttttctt tgtttttttc acctttttgt tgtcaatcat agcttacacc
      241 actatcaggg gatatcagac gcttgcccct tacggtaatt tcaaaacagt atatctggta
      301 tccatggttg tactttctgt cgtcatgttt gcaggtatcg gacttgaaaa tcagcttccg
      361 cacggcttag ccaaagggct gagttttgtg ggtcacacct ttttcattgt agctgcttac
      421 ctgtttattt cttttctgct gacagatata ttcagggcgg tcaatcattt cgtgccgctg
      481 gtaaccaata ttcaggcatt ccggctggga gcaatggtgg gcacgctggc tattctaact
      541 gtactgctgg tatggggtaa ctataaattc aatcatccgc agaaagtaca gctcactatc
      601 cacagcaaca aatcccataa aaaaaaccgg gagattaaaa ttgtggcagt aagcgacata
      661 cacctcggca cttccattga caaaaaaaga ctccggcagt atgtcgacct catcaatgcc
      721 gaaaaaccgg atcttgtact cattgccggc gatctgatcg accggtcgat taagcccgtc
      781 atcagtcaga aaatggacga agaactccgc accattcagg caccgatggg cgtgtatgtc
      841 attttcggaa atcatgaata ttacggcgaa ggcgatcatc acattgccga tttttaccgt
      901 aaatcaaaca tcacactgct cagagataca tcagtggtta ttaacaacga actgctcgtg
      961 gtaggtcgcg acgacaagca caaccccaac cgccggcacc tgaaagagct ttttcctccg
     1021 gatacagata aaccagtgat cgtgctcgac catcagccat accatctgca ggaagcggaa
     1081 agcagtcgtg tggatttaca aatttcggga cacacccaca acggtcagtt ttttccggcc
     1141 aatctgattg taaaaagtat gttcgaactg ggttatggtc acctgaaaaa gggtaatacg
     1201 catttttacg tatcgtcggg gcttggccta tggggaccac agtatcggtt gggaagccaa
     1261 agtgagttag tagtggtgaa tttcaggttt taataattca tccaccctca ccacttcata
     1321 tcctcttgtt ttcaattctt tgatcaaatc atccaggcga taataaaatt tgtcggtacg
     1381 agtggcgtgt gtgccgatgt gaagcagcaa caggttgccg ttgagtgtat tttccttttc
     1441 gaactgcaga atgttgttat aaatcacttc cgacgatttg taggaattca tttcgggcgt
     1501 ggtatagtcg gcattggagc ctgtgccggg tgtaaaattc accacctgta cgttcatctg
     1561 ctttgcccag tcgcttattt cgctgttata ccattcataa ggcgggatga aatagcgcgg
     1621 ctcgtctatt ttaagtcctg ctttttccat tgcgcggtag ttattttgca ggtctttctc
     1681 aaaagaatag cttgtaacca acgtcagatc gcgattttcc cagtcagcgt aaagcaggtg
     1741 tttatcggaa tgcggtgcca tgtaatgacc ctcctgctga agttgttttg taacctgcgg
     1801 aaacttccga tagaaatcgc ccgtcaggaa aaagctcgct ttcacaccat ttttcttcag
     1861 ggtttttgaa atcgttgcag ctccatcggc aaattcatgt ccggtgaaga cgagtgccaa
     1921 tttcctttcc aacggattac cgcgcacaat accgccctga accactttgc gggcaatggc
     1981 tttactgttc agatagctca ggtagaaagt aagtgaggct gttccgtcca tggtgggttc
     2041 gttggtcgca taatcggcaa aatcatcgtg ataaaccacg tagtccgact ggaaaggagc
     2101 gtacatatct tcttttgaaa gatgcacacc ccgcaacgag ttgaaaatcg gtgcataaat
     2161 cggtccgtcc actattccac cactgatggg gtaattccgt aatttggtaa aagcagagtg
     2221 cgggtcagaa ggcgtatcgc cgttagcggg ataatccact atcatgcagg ttccccacgg
     2281 attgacgccg aaaagccagt cgcgcatgga cgtttccatt tccacgaagg tatcatcgcc
     2341 tgtgagttcg tgatagaggc ggcattgtgt aatcagacca atggtcagat tattcgaaca
     2401 ccagatgaaa ggaactccgt tcataaatgc attgtccgca gcccggtctt tcacccgctg
     2461 caatccgctg cgcatattcc ttataaattc acggctgata cggctatttg cgttttgttt
     2521 tgcgaggtga taatgcccca gattaacgaa aggataccac tgataatgac gcgccgaatc
     2581 agctcccatc cagggagtta ccggttccat gcgaccgtaa ttcaccgcct gattcaggtt
     2641 ttcggaggat ttcgtcagta aatacatttc ggcagccgcc agctgcatat cgtcggccca
     2701 gttatcttct tcgtaaaagt atggcgaaac cgtgcaggct gtctgacata ctccggggtt
     2761 tgtcatccct ttgcggtagg catgagcagc tttcagggca atattttttg aaaattcggg
     2821 ataatatttt gccaaaaccc gggagccaag tgcgaaggaa gaagaatatt ttcccagggt
     2881 ggaagccagt ccggtgcttc ggtttttgta ttgccgcaat ccctgcggag aagggctgca
     2941 gaaataaacc ggacgggcat tgccggctcc ccagccgtag tccactttct gttcggaagg
     3001 aatggtcatt cccacgtgat cgcggtcgtc ggccagctga ttgaagtacg tcagcgaatc
     3061 gggattcatt ttcaccagcc agtccagtcc ccattttgct tcgtcgagta tgtcgggaat
     3121 gccgttggaa ccgggatcgc cgttggcatc gaaattatcg ccaaatgatt caggatgctg
     3181 tgtgtaggca aaaagcatct ggtaaaccgc attggccgaa gtagttacat actgcaggta
     3241 atcggaagca tcgtgccatc cgccggtggt attgtagtat tttccgtcgc gttttccttc
     3301 cggatcgccc acggagatgc catcggtagt atgacaggag tccttcagaa acggattata
     3361 gccgcatcgc tgctggcgca tgtattgcag gggaatgtcg gctgctccgg cgtaaatatc
     3421 gtttccgatg cggaaaacag acgattcggt ttcgtttacc ctgattttgt aagagcccgg
     3481 aaggttgaaa cccgagaaat caagtcgggc agatgtattg aaagcaggct gcggtccggt
     3541 ttgttttact ttatctgatg aatatacaat gctgcccgaa gcgacatcca cgagatcaaa
     3601 ttcggtaatc agccgtgaat ccttacttat ccacacagcc actttcacat cgtcttccag
     3661 gtaaccaatc tgattgacac gaatccatga ttgagccgtt acaacagaaa aacttattat
     3721 aaatacggta aaaagtgaaa gtatcttttt cataaggaaa tgatcttttt atatgcacga
     3781 caagttagaa aacttttttt aaaaaagtgt gtttttctat cttttctttt tgaaaaattc
     3841 cttcatcagt gcgctgcatt cctcttcgag cacgccggca actacttctg ttttggggtg
     3901 aatggcatcc ggggcaaagc gggtgaatcc ccgtttttcg tcggatgcac cgtaaacaat
     3961 gcggcttatc tgcgaccagc ccaatgcacc tgcgcacatc acacaaggtt ccactgtcac
     4021 gtagagcgta caatcggtga ggtattttcc gcccagaaaa ttggcggcgg cggttatagc
     4081 ctgcatttcg gcgtgggcag tcacatcgat gagggtttcg gtgaggttgt gcccgcgggc
     4141 aatgatgcgg tcgttgcaca cgatgacagc tcctacaggc acctcatcgc gccggaatgc
     4201 ctgccgggct tcatcgagag cctgacgcat aaagacctga tggcccccta aatcaccccc
     4261 agcggaattt ggtatgtgtg attgaatatg tgattgaaaa tttgacattt aaccttagat
     4321 taaaaaaagt tctgaatgtc ggacttacaa aaagaaaaaa aataaaagtt gaacgtttgc
     4381 agttctcaaa agtcccccgt agtttatccc gcatggcggg aggggattta gggggctgta
     4441 tcctcttcca ctaccagatt ttcgatgata aagccctgcc gttcgctggt atttttaccc
     4501 atgtagaatg aaagcaaagc ctgcacggca tcttcgcggc gaagttttac ctgctccagg
     4561 cgaatatcgg cgccaataaa attacggaac tcatcgggcg aaatttcacc caaccctttg
     4621 aaccgggtta tttccggatt gggtccgagt gtctgaatgg cgtccacccg ttcttcttcg
     4681 ctgtaacaat agcgtgtttc ttttttattc cgaaccctga aaaggggtgt ctgaaggatg
     4741 taaacgtgtc cttttttgat cagatcgggg aagaactgca ggaaaaacgt gataatgagc
     4801 aggcggatgt gcattccgtc cacgtcggca tcggttgcta cgattacttt gttgtagcgc
     4861 agaccttcaa tgccgtcttc gatgttgagt gctgcctgaa ggaggttaaa ctcctcattt
     4921 tcatacacta cccttttggt gagtccgaag ctgttcagcg gtttaccgcg caggctgaaa
     4981 actgcctgtg tgttcacatc ccggcttttg gtgatggagc cgcttgccga gtcgccttcg
     5041 gtgatgaaga tggacgaatc ttccatcagc ttatttttgg cttcgtcttt caccggagca
     5101 tcgttgaagt gatagcggca gtcgcgcagc ttgcggttgt gaaggctaac cttttttgcc
     5161 cgttcgcgtg cctgtttggt tactccggct atggctttgc gttccttttc agattcctga
     5221 attttctgca gaagaatatc agcagtttcg ccgtttcggt ggaggtagtt gtccagctct
     5281 ttttttacaa aatcggaaat gaatttattg accgtaatgc cgtttttgtg aatatctctc
     5341 gatccgagtt tggttttggt ctgcgattca aaaaccggct cttcgactcc gatggctaca
     5401 gcggctacta ttccgttgcg tatatcggtg atttccatgt ttttgttgta aaactccttg
     5461 atggtacgcg caaccgcctc acggaatgcg ctctgatgcg taccgccctg ggtggtgtgc
     5521 tgtccgttga cgaatgaata gtactcttcg ccatactgat tgctgtgggt aaaggctatt
     5581 tcgatgtctt ctcccctgag gtgaacgatg ggatagagcg attcggaagt catattttcg
     5641 ttcagcaaat cgatcagacc gtttttggaa ctgattttct ttccgttgta gttgagtacg
     5701 aggcctgtat tgaggtaagc atagttccga agcatggttt ctacaaattc gtccctgtac
     5761 tcataattcg gaaacagtgt tgcgtcgggt ataaactccg tgagagtacc attctctttt
     5821 ttcaatcctt caaaatcctg ttcttcaacc agttttcctt cggaaaaggt ggctttttta
     5881 gattttcctt cacggtaact ctccaccatg aagcttgacg ataacgcatt cacagctttg
     5941 gttccgacac cgtttaaccc aaccgatttt ttgaagacct gtgaatcaaa ttttccgccg
     6001 gtattcatga tggatacggc tttcaccacg ctattgagcg gaatgccccg tccgtaatcg
     6061 cgcacgctca ctctttcgtt ttcgatacgg atttcgattg ttttgccgtg tcccatccga
     6121 aattcgtcga tcgaattatc aatcacctct ttgagaagta cataaatacc atcatccgca
     6181 tgcgatccgt cgcccagttt gccgatatac atccccggtc ggcgacggat gtgctccagc
     6241 ccttcgaggg tgataatatt accgtcgcca tatacaacat cgatcttttc caaattcttt
     6301 aaatcttcca tttttgatat attatcttgt taaccaactt tcaggattta ccatctgact
     6361 accgttgtag aggttgaact gcagttctgc ctgaccgctt tcatcgtcgg tgtagatttt
     6421 tccgatgggc tggcgtgccg aaacctgctc accggagcgt acaaacactt cggagaggtt
     6481 ggcgtagagt gtgcggtagt ttccgtgttg cacgatgact gcatttccgg tgccgggcag
     6541 tgagaaaatg cgggtgacta ccccttcgaa tacagcgcgt gcgttggtac ctgccggcga
     6601 tcggaagtac acgcctttgt tattgatagt cacgtatttg agttcgggat gcggctgagt
     6661 gccgtaatgt ccgcgtatat accctctgtc caccggccag ggcaatcgtc cctggttgtt
     6721 ggcaaagtta cccgaaagca atctctcttc gcgggtatag gccgatacgg attcggcatt
     6781 tccggacctg gtggttgttg ctacttcggt ttttgacgta gtttcaacgg gtttggtggt
     6841 ttcggcgggt tttgcttcag caatggtttc tgttttctta ttttgtgatt cagccttttt
     6901 gcgctgctcg gcagctcgtg cttcggccat tttacgttct tcgatcttgc ggcgagcctc
     6961 ctgctctgcc cttattcggg cttcttcggc gcggcggcgt gcttcggcgc ggcggatttc
     7021 ctcctctatc acctgctgaa ttttattatc tatctgatct tttttacgct gatgtgccct
     7081 gtactcttcg ctcagccttt tttcttcctg ctggagtgag gcgtacatac tcttctcttc
     7141 gctttcatct tttttcagct tttcggcttc gagttgtttt gctttcaggg cacccatttt
     7201 ggcgagttta ttctgatcga ggctgtcggt tttgagagca atttgagttt ttacctgttc
     7261 aatctgtttg acctgttctt ttttataatc ggtaaattcg cgcagatagc gcacccttcg
     7321 tattgtctgg tcgaagtctt tggccgaaag cagaaacatg agtctggaat atgaattcat
     7381 gttggcctgg gttttccgga taagccgggc gtaatctccc ttcagttttt ccagctgttt
     7441 ttcgagttca atgcgctcgg cggtgaggct gttcatcttt ctgtcgagca catttatctc
     7501 gtcgttgtaa tttttgatga ggtttcttcg ttcgttcagg ttttttttga gtgcattgag
     7561 ttttttgagc gatgcctttt cgtttttctg ggtttctttg agcaacttat tggtttcgtt
     7621 gatgcgttcc tgtatctcca tgcgctgttt tttgagtgtt tccatcgatt gtgcccggac
     7681 accgaaatta caggataaaa tcagcgaaat gattgtgatt aaatatgtta tctttttgtt
     7741 catcaactat tattttctga ataaggaatt aatgtttccg cgtgtgtacc gactcaggtt
     7801 ggttggttcc atcactaccg gtttgttgaa ttccacctgc tcaatggtaa aatggaacga
     7861 cgatttttcc tcgcctttgt tggcatcgat ggttattttt tgaggaaaaa gcatgccgtc
     7921 ggtttttttg aaatccgaat agtcggtttt catggtgtaa gtggaatcgg cagaaagaat
     7981 cataacggct gcaatgcggc taagcagcac atccacgagc acttcctggt tgaaggattc
     8041 ggtctgcagg gtaagtgatt tgccgggggt attgtctttc cagttgaact catcgggtag
     8101 aaacacctta tcgccgggta cgaaaagccg gttgctgatg agcgactgga tgacgtcgta
     8161 attgacggtg acaccgagtg tattcctgaa tatgctgtaa ttgctttcgt agaacctttt
     8221 attcatttta tccaccacaa taagggtgga aggcgtcatt tcgagtcgga agagttccac
     8281 gcccataaag ggctgaatgg acaggtagag tgcggagtcg gatacaattt tgcacgatgc
     8341 ctttacgtcc atctggcgcc ctttgaaatc cacataaacg gacattttac tggcgttggc
     8401 cgttcggaat gccggttgcg cgagctggag ctgtttcacc atatcggcag gtgtgaactc
     8461 gtgagctgct tctgtgggtg tggtggcggt ttgggtaggt tttacagccg ttttggtggt
     8521 tttgcaggct ccgaagagga ctattgcagc tgccacaaca agtattctga tcagtttcgg
     8581 tttcatttta tttcattttt tgcttgatcc gtttttttga ttagttcggc gttgttttca
     8641 tttagtttca acgccttttg ccacatttcc agggcttttt cggtgttttt cagtgcgtaa
     8701 taaatatcgc cgctgtgctc cagaatcact tccgatattt cgtcctcttt catgttgctt
     8761 accgccttat cgatgtaaat gcgggccagg ttataatttc cctgtttgta gaatatccag
     8821 gcgtaggtat cgaggtaagt gctgttgttg ggctccagct ccacggtttt tgcactcatt
     8881 ctttcggctt ttctgaggtc agttccttct acggagaggt aataggcgta gttgttcatg
     8941 atgtacacgt tggccggatt ggcttccagg gctttttcgt agttttcgaa agcgtgttcc
     9001 ttatcgtcca ttttgaaata aatatcgcct atctgcccat agaagctgct taaaacaggt
     9061 ggtgcagccg atgtcaggtt ttcgatggcc gtgaaatttg tttgtagtgc ttcttcatat
     9121 ttctgctgct gatacagagc tatagagcgg tagaaataga attccggcac ttcggggagt
     9181 tcgtgcagag cgcgttcggt gagcgatagc actccggtag tatcgttttt ctcggtcaga
     9241 atttgcagcg aatttttcca ggcggcttcg tttttcggat tgatattgat cacactttcc
     9301 agttcctcga gtgcttcggc ggtccgtttc tgatgctgaa gaaaaagtgc atagtaagca
     9361 tgcgccattt catcgagcgg atacatgtca atgagtattt taaaaaggtt ttcggtttcg
     9421 ccgattttct ggttggaacc caggagctga tccacgtact ggcccaggat atccattttg
     9481 gtatcagacg gcaattccgg atttttcagg gcagatacga tagactccag cgccttgtcg
     9541 tcctgcctgt tcagcttgta atagttggag agcgatacgt acacaaaggg atttccggga
     9601 tcgtctctga gtatctgctg atatatttca taggctttgc ggggctgctt ctgatccatg
     9661 tagatatcac ccagcaacac gcggtagcgg ctttcctgcg gatacttctg tgtgagtttc
     9721 agaatctctt cgatagcctt attgtccttt ttcaggaggg catacagttg aaatttttct
     9781 attgacaggt attcattgat gcctgtaaaa acctccagct ggttgagtgc accgatggct
     9841 ttatcaaatt ccccggtctg cttatacagt gacgagagca tggtgtacac ctcttcgcgc
     9901 tgcggaaagc gttttcggag ttcttcagcc tgttcgatgg ctgttgggta aagtttttgt
     9961 gtgctgagca tgctgatata ctgcgttctg taccaccagt tttcgggtgc ggcttctact
    10021 gactttctga aagcacgcat cgattcggtt ggccagttga ggcgagcata tgcatatcct
    10081 gtttcgctct gggcggcagc gctcgttgaa tctatctcaa gacacattct caatgcatcg
    10141 atttgtgcat cgtactcctg tatattcttt agccgttgtg cctcgaaaaa ataatagtca
    10201 aagcgtgcct gttggtcggc tgaaaggcga actgcaaccg gaacgctcat tttgcctttt
    10261 ggaagagcaa acgccggaat tgtcagcagc aggatgagga gaaaagcccc tctaaatctc
    10321 cccaaagggg agacttttgc aatagtgccg gtatgttgtt ttttactctt actcataact
    10381 cataactcat atctcattac tcatatctca ttactcatat ctcattactc a
//
LOCUS       DAORKP010000078        10364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_788_length_10364_cov_8.341449, whole genome shotgun sequence.
ACCESSION   DAORKP010000078 DAORKP010000000
VERSION     DAORKP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_10364_cov_8.341449"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLN75_05710"
     CDS             <1..764
                     /locus_tag="PLN75_05710"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRG03645.1"
                     /translation="ARCDEFKTPEGRKLAYETMKKEGIDALVVIGGDGTFTGARVFAQ
                     EFNIPIIGLPGTIDNDLFGTDSTIGYDTALNTIVEAVDKIRDTASSHERLFFIEVMGR
                     DAGFLALNSALASGAEAAIIPERETKVDQLAELIQNGFRKSKNSSIVIVAESEVTGGA
                     NGLAERMHREYPGYDVRVTILGHIQRGGSPTAYDRTLASRMGVAAIDALMDEQRSIMI
                     GIVNDEIVHVPFTKAIKDDKPVNERLLGVLQMLSI"
     gene            850..1332
                     /locus_tag="PLN75_05715"
     CDS             850..1332
                     /locus_tag="PLN75_05715"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRG03646.1"
                     /translation="MISLLFVCLGNICRSPMAQTVFEKIIENQGVEDQFRVDSAGLIN
                     YHEGEKADSRMRSHARARGYEITHLSRPVQKKDFEKFDYIVGMDDQNIRALNHMTSSG
                     EEQAKIIKMTDFLQKFQAVSVPDPYYEGDAGFVKVIDLLEDACEGLFRHLQIKDFGNK
                     "
     gene            1413..2177
                     /locus_tag="PLN75_05720"
     CDS             1413..2177
                     /locus_tag="PLN75_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03647.1"
                     /translation="MKKISFKLSAVLLLTLYAVSCAKEPDLGTVSLDPSSKTVTFGQV
                     FNIKPVFSATGEAKNKTYTWKSSADSIASIKMVSGGFGEVTPKRIGQATMTYASTDGK
                     ITKTAIITVDPRSTILNGVYFKNGATQSEINNNMLSGFSPSTLESTSSLLVFASGSST
                     VSKLIYELDTSNKLKALYVILNNTADNKKSAEDYLAERFMNTGKSQGGILYYKNTGYV
                     ASNSVPLKTALGLFFDLTINATQYPLGVKIMDGSNL"
     gene            2326..2952
                     /locus_tag="PLN75_05725"
     CDS             2326..2952
                     /locus_tag="PLN75_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03648.1"
                     /translation="MLENLDEELVRCRPCGYVMKKSELENVGVCPACGLPHTVFEPYR
                     EKVSPKRLFILSLDIHPIVIHLSQTFVAMIPVLMITHLLFPNFIPDILSPVIDFSIFV
                     FPITLIGAFLSGILDGLTRFKTLDTPLLKSKIYYGAAILILSFAMLLSLLFMPEKYTF
                     IDLILSLGALFCAVKLGMMGKELLNVILPGTYQRRKKKVVVKKPAVNQ"
     gene            complement(3025..4044)
                     /gene="pheS"
                     /locus_tag="PLN75_05730"
     CDS             complement(3025..4044)
                     /gene="pheS"
                     /locus_tag="PLN75_05730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRG03649.1"
                     /translation="MKVRIKQLTDEINALYANTMDEVEILRIKYLSKKGEVSALFNDF
                     RNVPNDEKREIGQLLNDLKNLAQDKINGMKETLSATDKMEKRNDLTRTAEPVKLGTRH
                     PLMLVKQEIAEIFSRIGFTISEGPEIEDDWHVFGALNFPPEHPARDMQDTFFIEKNPD
                     VLLRTHTSSVQTRIMSSQQPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALLYFAKEMFGADTQIRLRPSYFPFTEPSAEMDISCNLCGGEGCAFCKYTGWV
                     EILGCGMVDPNVLKNCSIDPEIYSGYAFGMGVERITNLKYQVKDLRLFSENDVRFLEE
                     FKGAY"
     gene            4218..4892
                     /locus_tag="PLN75_05735"
     CDS             4218..4892
                     /locus_tag="PLN75_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRG03650.1"
                     /translation="MKEVLDFLRGLRENNNREWFAAHKSEYEIAKKEFENVCQSLILK
                     IGEFDDKILNLTPQECVFRIYRDIRFSNDKTPYKNHFGTYIAFPGGRKSPRGGYYLHI
                     DPIEGCFFGGGIWSPEPAVLKVLREGIYYNYEEFEEIINEPEFKKIYGNSFYNEDKLK
                     TLPKGFPADFANPEILKLKHYFVSLDLNENEITDPNFTNHLAWLARVAYPLNQFLNYA
                     VDESKD"
     gene            4917..6926
                     /locus_tag="PLN75_05740"
     CDS             4917..6926
                     /locus_tag="PLN75_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRG03651.1"
                     /translation="MKTKPILPLVKSDPWLEPYADAIQGRYDYYLQVEKKLTGGNMKL
                     SDFATGYLYFGLHHVPEGWIFREWAPNATAIYMIGDFNNWKKDEKYRLIPTNDGIWEI
                     HLDENALKHKQLYKLLIEWEGGAGERIPAWCRRVVQDEQTKIFSAQVWNPENPYQFKV
                     EKFVPDTKPLLIYECHIGMSSSEEKVSTYDEFRRTVLPRVVQNGYNCIQIMAIQEHPY
                     YGSFGYHVSSFFAASSRFGTPEELKRLIDQAHEMGVAVIMDIVHSHAVKNEVEGLGRF
                     DGTPYQYFHGGDRREHPAWDSLCFDYAKPSVLHFLLSNCKFWLDEYKFDGFRFDGVTS
                     MLYRSHGLGEDFNGYGSYYNLNQDGDAICYLTLANKMIHQVNPNAITIAEEVSGMPGL
                     AIKPEDGGIGFDYRMAMGIPDFWIKYIKEVKDEDWKAGHIFWELTNRRADERTISYAE
                     SHDQALVGDKTIIFRLIDADMYWHMQRGDQNLTVERGIALHKMIRLITATTINGGYLN
                     FMGNEFGHPEWIDFPREGNGWSCKYARRQWELVDNPDFIYHGLGEFDRAMLELIHSVP
                     AFNETPIYQLWDNEGDQVVAYQRENLVFVFNFNGTNSYTDYGILAPKGSYKVILNTDN
                     PQFDGHGLIDENVEHFTQKASKEFMGKEWLKLYIPARSAFVLRKL"
     gene            6953..7732
                     /locus_tag="PLN75_05745"
     CDS             6953..7732
                     /locus_tag="PLN75_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRG03652.1"
                     /translation="MKSLSLKILSTNLWKEYAENIEANLFHKFESLKESELSTENFFF
                     YVSVSAVFSSKIEGEGIDLDSFLKHKKLGVSYQHDYTRKIDDLYEAYVFAQNHSLTEK
                     TLAEVHRHLSKNLLHTSKQGVYRSGNMFVMTADGKIEYVAPSPYVLKFELSDFFEDLN
                     ALLNADLSFEQSLFFASQLHLILVKIHPFEDGNGRTARLLEKWFLAEKLGEKAWFLQS
                     EKTYYDNQNGYYAALRALGLEYETLDYSRALPFLTLLPKSL"
     gene            7851..8303
                     /locus_tag="PLN75_05750"
     CDS             7851..8303
                     /locus_tag="PLN75_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03653.1"
                     /translation="MELNEAKKDYNEPMHTTEHILNQTMVRMFGCGRSMNAHIERKKS
                     KCDYTLSQAPTEAQIEEITNRVNDVIGQQLPVTESLMNREEASRIADLSKLSADASEI
                     LRIIKVGEYDACACIGQHVKNTSEIGRFEILTHDFENGRWRVRWRVVN"
     gene            8352..9233
                     /locus_tag="PLN75_05755"
     CDS             8352..9233
                     /locus_tag="PLN75_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRG03654.1"
                     /translation="MNVLIAGGTGFIGNYLKNRFEASGHIVRLVSRSGMDVSWNLKDL
                     IPELEKTDVLINLAGKSINSRFTEKNKELILQSRLKTTGILNEAVAACKNPPALWINA
                     SATGIYKHTGQNQTLNESSMDFASDFLGNVVQLWEREFFGTSIPGVRKTAIRTSVVLG
                     KSGGAYPIFKLLAITGLGGKQGSGAQMVSWIHIEDYYRIILFIIEESGISGVINASAP
                     FPVTNKIFMKTLRKAKRFPVGIPAPEWAIKLGTGIVGVDSSLILASSNVNSEILMKNG
                     FEFRFPTVEKAFLDLTQ"
     gene            9264..9965
                     /gene="mtgA"
                     /locus_tag="PLN75_05760"
     CDS             9264..9965
                     /gene="mtgA"
                     /locus_tag="PLN75_05760"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRG03655.1"
                     /translation="MKKKKTFFSRLRSWVMKLLLIMFAGSILAVLIARFVPVYFTPLM
                     FIRAAESVFSGEKPTFERNWTSIHNISPNMIQAVVASEDNLFLEHNGFSFNDIEKAIE
                     HNKKGKRIRGGSTISQQTAKNVFLWPHRSWIRKGLEAYFTVLIEFFWTKQRIMEVYLN
                     VAETGDGMYGVQAAAENYFDKDAKNLSKSQSALIAVCLPNPRKFNPAKPSSYIQRRKN
                     QIVSLMGKLKQVEFD"
BASE COUNT         3127 a         2023 c         2178 g         3036 t
ORIGIN      
        1 ctgcgcgttg tgatgaattc aaaacacccg aaggacgtaa actggcttac gaaacgatga
       61 aaaaggaggg aattgatgca cttgttgtaa ttggcggcga tggcactttc accggtgccc
      121 gcgtatttgc acaagagttc aatatcccca taataggcct tccgggtact attgacaacg
      181 acctctttgg tacagactca acgattggct acgatacagc cctcaatacg atagtagaag
      241 ctgtggataa aatccgcgat acagcctcat cacacgagcg ccttttcttt atcgaagtaa
      301 tggggcgcga tgcaggattt ctggccctca acagcgcact ggcatcagga gccgaagcgg
      361 ccatcattcc cgaacgtgaa accaaagtgg accagctggc cgagcttatc caaaacggat
      421 tcagaaaatc aaaaaacagc agcattgtca ttgtagccga aagtgaagtg accggaggag
      481 ccaacggact ggccgaacgt atgcatcggg agtatccggg ctacgacgta cgggtaacca
      541 tacttgggca cattcagcgc ggtggttcgc ccacagcata cgaccgtacg ctggcaagcc
      601 gcatgggagt ggcagccatt gatgcactga tggacgagca gcgaagcatt atgataggaa
      661 tagtgaacga cgaaatcgtg cacgttccct tcaccaaagc catcaaggat gataaaccgg
      721 tgaatgaaag attgctggga gtactgcaga tgctatcgat ataaaacatt aaaaaactta
      781 agccggtttg gaatcatcca aatcggtttt tttgatacaa ataaattctt ttttttctga
      841 aatgacaata tgatatctct tctttttgtt tgtctgggca atatatgcag gtcgccgatg
      901 gctcagactg tttttgaaaa aataattgaa aatcaaggtg ttgaagatca attccgtgtt
      961 gattccgcag gattgattaa ctatcacgag ggcgaaaaag cagactcacg catgcgttca
     1021 catgcccggg caagagggta tgaaatcacc cacctttcac gtccggttca gaaaaaggat
     1081 tttgagaaat ttgattatat cgtgggtatg gacgaccaga atatccgcgc acttaaccat
     1141 atgacttcat ccggagaaga acaggcaaaa atcatcaaaa tgacagattt tcttcagaaa
     1201 tttcaggcag tctccgtgcc cgatccttac tacgagggcg atgccggttt cgtgaaagtc
     1261 attgacctgc tggaagatgc ctgcgaaggt ctcttcaggc atttgcaaat caaagatttc
     1321 gggaataaat aaacctaatg aaacgtttcg gtgttataaa taagatacat ccaagttttc
     1381 taaattaaaa atttcccgta aaaataataa ttatgaaaaa aatatccttt aagctatctg
     1441 ctgttttgct gctaacattg tatgcagttt catgtgctaa agaacctgat ttaggcactg
     1501 tgtcactcga tccgtcatct aaaaccgtca catttggaca ggttttcaac attaagcctg
     1561 tatttagtgc tacaggagaa gctaaaaata aaacctatac ctggaaatcg agtgcagatt
     1621 ccatagcaag tattaaaatg gtgtcaggtg gtttcggcga agttaccccg aagcgtatcg
     1681 gacaagctac catgacttat gcttctaccg acggaaaaat aactaaaaca gccattatta
     1741 cggtagatcc gcggtccaca attctgaatg gagtttactt caaaaatggc gctacacaaa
     1801 gcgagatcaa caacaacatg ctgtcaggat ttagccccag cacgcttgaa tcaaccagct
     1861 cgttgctcgt ttttgcatcc ggaagttcta cagtaagcaa actgatatac gaacttgata
     1921 caagcaacaa gctgaaagct ctatacgtga ttttgaacaa cacggctgat aataagaaat
     1981 cggctgaaga ttatctggca gaaagattta tgaataccgg aaaatcgcag ggtggaattt
     2041 tatattacaa aaacaccgga tacgttgcca gcaattctgt acccctgaaa actgcattgg
     2101 gtttgttttt tgatctgact atcaatgcta ctcagtatcc tttgggggtg aaaattatgg
     2161 acggttcaaa tttataatgc attctgaaaa ttcaaaaaac tgaaagagac cggcagtcat
     2221 tggttgtcgg tctcttgttt ttttttgtat ttttttcaac gttgcgttta ataattgata
     2281 atatcttgtt atttttgcaa ttaaattaat aaaaaatcag gtattatgtt agaaaattta
     2341 gatgaagaac tggtacgctg tcgtccgtgt ggatatgtaa tgaaaaagag tgaactggaa
     2401 aatgttggag tgtgtccggc ttgcggattg cctcacaccg ttttcgagcc gtatcgtgaa
     2461 aaagtatcac ccaaacgact ttttatttta agtcttgata ttcacccgat tgtgattcac
     2521 ctttcgcaga cattcgtagc tatgattccg gtactgatga tcactcatct gctgttcccg
     2581 aacttcattc ccgatattct ttcgcctgtc attgattttt cgatttttgt atttcccatt
     2641 acacttattg gtgcctttct ttcgggtatt cttgatggtt tgacccgctt caaaacgctg
     2701 gatactccgc tgttgaaatc aaagatttat tacggagcgg ctatcctcat tctttcattt
     2761 gctatgctgc tgtccttgct ttttatgccc gaaaagtaca cttttatcga tctcatctta
     2821 agtctgggtg ctttgttctg tgctgtgaaa ctgggaatga tgggcaaaga attgctcaat
     2881 gtaattttac cgggcacata tcagcgaaga aagaaaaaag tggtagtaaa gaaaccggct
     2941 gtaaatcagt agaataatat tgctttttaa aacataaaaa aagccacttt atcggtggct
     3001 tttttttatg tatatccatt attatcagta ggctcctttg aattcttcga gaaaacgtac
     3061 atcgttttca gaaaaaaggc gtaggtcttt cacctgatat ttcaggttgg taatacgttc
     3121 cacacccatc ccgaaagcat atccggagta aatttcggga tctatactgc agtttttgag
     3181 tacgttggga tctaccatgc cgcatcccag aatttctacc caaccggtgt atttacagaa
     3241 tgcacatcct tctcctccgc acaaattgca ggaaatgtcc atttcggcac ttggctcggt
     3301 aaacggaaaa taggacgggc gcagacgaat ctgagtgtcg gctccgaaca tttctttggc
     3361 aaaataaagc agcgcttgtt tcaaatctgc aaaagataca tttttatcga tgtaaagccc
     3421 ttctacctgg tgaaagaaac aatgagcacg gtatgaaatg gcttcgttcc gatacacgcg
     3481 gcccggaaat atcatccgga taggaggttg ctgcgatgac atgatgcggg tttgaaccga
     3541 cgatgtatgc gtgcgaagca atacgtccgg atttttttcg ataaagaaag tgtcctgcat
     3601 atcgcgggca gggtgttcag gaggaaagtt gagcgctccg aatacgtgcc agtcgtcttc
     3661 tatctctggt ccttcggata tagtaaagcc tattcttgag aatatttccg caatttcctg
     3721 ttttacaagc atcaacgggt gacgtgtgcc cagcttcacc ggctcggctg tacgggtaag
     3781 gtcgtttctt ttctccattt tgtcggtagc agaaagcgtt tccttcattc cgttaatctt
     3841 atcctgagcc agatttttca gatcgttcag cagctgaccg atttcccgct tttcatcgtt
     3901 aggtacgttt ctgaaatcat tgaaaagtgc tgaaacttca ccttttttac tcagatattt
     3961 aatacgtaaa atttctactt catccattgt atttgcatac aaggcattta tttcatctgt
     4021 taattgtttg attctgactt tcatctcctt ttttgttatc cttccgggtt tattttacat
     4081 gttttttacc cgattgaaat ttgatttgca aaattaatca tttttctcca aactgacaga
     4141 gaaccggaaa aaatatctca tatacgaagt aaaattgtaa tttttcagtt atatttgtaa
     4201 ataaattttg atgaataatg aaagaggtac ttgattttct cagaggattg agagagaata
     4261 acaacaggga atggtttgcc gctcacaaaa gcgaatatga aattgcgaaa aaagaatttg
     4321 agaacgtatg tcaatccctg atattaaaaa tcggagaatt tgatgataag attctcaatt
     4381 taactcctca ggaatgtgtt ttcaggattt accgcgatat ccgtttcagt aacgataaaa
     4441 ctccctataa aaaccatttt ggaacctaca ttgccttccc gggcggcaga aaaagtcctc
     4501 ggggcggata ttaccttcat atcgatccca tagaaggttg tttcttcgga ggcggcattt
     4561 ggagtcccga accggctgta ctcaaagtgc ttcgtgaagg tatttattat aattatgaag
     4621 agtttgaaga gataatcaac gaaccggagt tcaaaaaaat atatggtaat tccttttaca
     4681 acgaggataa gctgaaaact cttccaaaag gatttccggc agattttgcc aatcctgaaa
     4741 ttctgaaact gaaacattat tttgtttcac tcgatttgaa tgaaaacgag ataaccgacc
     4801 cgaattttac aaatcactta gcctggctgg ccagagtggc ttatccgttg aaccagtttc
     4861 ttaactatgc tgtagatgaa tcaaaagact aaaaaacaga taattaaaaa ataaatatga
     4921 aaacaaaacc gattcttcct ttagttaaat cagacccttg gctggaacct tatgccgacg
     4981 ctattcaggg cagatacgat tactatctcc aggtggaaaa gaaattgaca ggtggcaata
     5041 tgaaattatc cgattttgcc acaggatatc tttactttgg tcttcatcac gtacctgaag
     5101 gatggatttt cagagaatgg gctcccaatg ctactgctat ctatatgatc ggtgatttta
     5161 ataactggaa aaaagatgaa aaatacaggt tgattccaac gaatgatgga atctgggaaa
     5221 tacaccttga cgaaaatgcg ctgaaacaca agcagcttta caaattgctg atagagtggg
     5281 aaggtggtgc aggggagcga attccggcct ggtgcagaag agttgtgcag gatgaacaaa
     5341 ccaaaatctt cagcgcacag gtctggaatc ccgaaaatcc gtatcagttt aaggtcgaaa
     5401 aatttgtacc cgataccaaa ccactgctga tttacgaatg ccacatcggc atgtcaagtt
     5461 ccgaggaaaa agtatccacc tacgatgagt ttcgccgaac tgttttacca cgcgtggttc
     5521 aaaacggcta caattgcatc cagataatgg ccatccagga acatccatac tacggatcat
     5581 ttggatacca tgtctccagt ttttttgcag catcatcacg ctttggtaca cccgaagagc
     5641 tgaaacgcct cattgatcag gcgcatgaaa tgggggtagc cgtaatcatg gatattgtgc
     5701 attcacacgc tgtaaaaaac gaagtggagg gtttggggcg ttttgacgga acaccatacc
     5761 agtattttca cgggggagat cgtcgcgaac atccggcatg ggattcgctc tgttttgatt
     5821 acgcaaaacc atccgtattg cacttcctgc tctccaactg taaattctgg ctcgatgaat
     5881 ataaattcga cggattccgg ttcgatggcg ttacctccat gctgtaccgt agccacggat
     5941 tgggtgaaga tttcaacggg tatggctctt attacaacct taatcaggat ggcgatgcta
     6001 tctgctacct gactttggct aacaaaatga ttcatcaggt aaatccgaac gccatcacca
     6061 tagccgaaga agtgagtgga atgcccgggc tggctattaa acccgaagac ggaggcattg
     6121 gctttgatta ccgcatggcc atgggaatac ccgatttctg gataaaatac atcaaggaag
     6181 tgaaagatga agactggaaa gccggacata tattctggga actcacaaac cgaagggcag
     6241 acgaacgaac catcagctat gccgaaagtc acgatcaggc actggtgggc gacaaaacca
     6301 tcatttttcg actcattgat gccgatatgt actggcacat gcagcgtgga gatcagaatc
     6361 tgactgtgga acgcggcatt gcactgcata aaatgattcg gttaataaca gccactacca
     6421 taaatggcgg ttatctcaac tttatgggca acgaattcgg acatccggag tggattgatt
     6481 ttccacgcga aggaaacggc tggtcctgca aatatgcacg caggcagtgg gaactggttg
     6541 ataaccccga ttttatttat catggtttgg gtgaatttga ccgcgcaatg ctcgaactga
     6601 tacattccgt tcccgctttc aacgaaacac ccatttatca gctctgggac aatgaaggcg
     6661 accaggtggt ggcttatcaa cgagaaaacc tggtatttgt gttcaatttc aacggaacaa
     6721 actcatacac cgactatgga attctggccc cgaaaggaag ctacaaagtg attctgaata
     6781 ccgataaccc gcaattcgat ggtcacggat tgatcgatga aaacgtagaa cattttactc
     6841 aaaaagcttc taaagaattt atgggcaaag aatggctcaa actttacatt ccggcacgct
     6901 cggcttttgt gcttaggaaa ctgtgagata gttgcttatt atattgtaat caatgaaatc
     6961 actaagcctc aaaatactgt caacaaactt atggaaagaa tatgctgaaa atattgaagc
     7021 gaatttgttc cataaatttg agagtctgaa agaaagtgaa ttgagtacag aaaatttctt
     7081 cttttatgtg tcagtttcag ctgtattttc atccaaaatt gagggtgaag gtattgattt
     7141 agacagtttt ttgaaacata aaaaattagg tgttagctat caacacgatt atacccggaa
     7201 aattgacgat ttatacgaag cttatgtttt tgctcaaaat cattctctga cagaaaaaac
     7261 actggccgaa gttcaccgac atttgagtaa aaatcttctt catacctcaa aacaaggagt
     7321 ttatagatcg ggaaatatgt ttgtgatgac agctgatggg aaaatagagt atgtagctcc
     7381 atcaccttat gtgttgaaat ttgaattaag tgattttttt gaagatttga atgctttgct
     7441 gaatgccgat ttaagttttg aacaatcgct tttttttgct tcgcaattgc atttaatact
     7501 tgtgaaaatt catccttttg aagatggaaa cggacgcaca gcgcgattgc tagaaaaatg
     7561 gtttctggct gaaaaattag gcgaaaaggc ctggttttta caatccgaaa agacctacta
     7621 cgataaccaa aacggctatt atgcagcttt aagagcattg ggtttagagt atgaaacgct
     7681 cgattacagt cgagcacttc cttttttaac tttattgcca aaatcgttgt agcaggttgc
     7741 ttctggtttt ctgatatgtt ttaaattttt attcgaatcc tcaactatac ctccctgcca
     7801 aagttaaatg actttggcag aattttgtaa atttatatta tgcaaaaaaa atggaactca
     7861 acgaagccaa aaaagactac aacgaaccca tgcacaccac cgaacacatt ctgaatcaga
     7921 ccatggttcg gatgttcggt tgcggccgtt cgatgaatgc acatatagag cgcaaaaaat
     7981 ccaagtgcga ctatacttta tcacaagcgc caaccgaagc gcaaattgaa gaaattacaa
     8041 atcgagttaa tgacgtcatt gggcagcagt tgcccgttac agaaagtttg atgaaccgtg
     8101 aggaagcctc acgcattgct gatttgtcta aactctctgc tgatgcatca gaaatattga
     8161 gaattatcaa agtgggcgaa tacgatgcat gtgcgtgtat cggacaacat gtgaaaaata
     8221 catccgaaat cggaaggttt gaaattctga cccatgattt tgaaaacggc cgctggcgtg
     8281 ttcgctggag ggtggtgaat taattcaaat caggttgaaa atcaggtgtg ataacttatt
     8341 aaattaacaa catgaatgta ttaattgcag gtggtacagg ttttattggt aattacctta
     8401 aaaaccgatt cgaagcatcc ggacacattg ttcgtttggt ttcccgcagc ggaatggatg
     8461 tttcctggaa tttgaaagac ttgattcctg aactcgaaaa aacggatgta ttgatcaatc
     8521 ttgccggaaa atcgatcaat tcacgcttca ctgaaaaaaa taaagagttg attttgcaat
     8581 cacgcttaaa aaccaccgga atactcaacg aggcagttgc agcttgcaaa aatccaccgg
     8641 ctttgtggat aaatgccagt gcaacaggca tctacaagca tacgggtcaa aatcagacat
     8701 tgaatgaatc atccatggat tttgcaagtg attttttggg aaatgtggtt cagctttggg
     8761 aacgcgagtt ttttggcact tcaattcccg gagtgagaaa aactgccatt cgaacatctg
     8821 tagttctggg taaatccggt ggggcttacc cgatttttaa gttgcttgca atcacaggat
     8881 taggaggcaa gcaaggcagt ggcgcgcaga tggtcagctg gattcacatc gaagattatt
     8941 atcggattat tctttttatc atcgaagaat ccggtatttc gggagtaata aatgcatcgg
     9001 ctccatttcc ggttacaaac aagattttca tgaaaacact ccgtaaagcc aaacgctttc
     9061 ctgttggaat tcccgcacct gaatgggcaa taaaacttgg caccggcatt gtaggagtag
     9121 attcttcgtt gattctcgca agttcaaatg taaattcaga gattttaatg aaaaatggtt
     9181 tcgaattcag attcccaacc gttgaaaaag catttctgga tttgacacaa taatatatac
     9241 atatataata tataatgatg acaatgaaaa agaaaaagac atttttttca agactcagaa
     9301 gctgggtgat gaagttgctg ttgattatgt ttgcgggtag tattctggct gtactcattg
     9361 cccgttttgt acctgtttat ttcactccgc tcatgtttat ccgggctgcc gaaagcgttt
     9421 tttcgggcga aaaaccaaca ttcgaacgca actggacatc tattcacaat atctcaccca
     9481 acatgattca ggcagtcgta gcttcagaag ataatttgtt tctcgaacac aatggattct
     9541 cttttaatga catagaaaaa gcaatcgaac acaataaaaa aggaaaacga attcgtggcg
     9601 gaagcactat ttcacagcaa acagccaaaa atgtatttct ctggcctcat cgttcatgga
     9661 ttcgtaaagg attggaagct tattttaccg tactgattga gtttttctgg actaagcagc
     9721 gaattatgga agtatatctg aatgtagcag aaaccggtga tggtatgtat ggagtgcagg
     9781 cagctgccga aaattacttt gataaagatg ccaaaaatct tagtaaatct cagtcggctt
     9841 taatcgcagt ttgtttgccc aatccgcgaa aattcaatcc cgcgaaacct tccagctata
     9901 ttcagcgacg taaaaaccag attgtcagtt tgatgggaaa attaaagcag gtagaatttg
     9961 attgaatact ttccgaaaat aacttttttc tttattttga acaatttttg tatatatcaa
    10021 acatattttc tcccgaacgt ttgtaagcgt tcgaatcata catgaatata tgtatgcaaa
    10081 tctttcctgt tttatccatc tgtttactca ttattttact cgaatgttcc gttcgttttg
    10141 tccatttttt gtccatttat tgtcattttc cagtcttttt agagtcattt agattgataa
    10201 ttgtgatcac cggagagagt tttgggtaga ttattcgttt tttgatttac aatttgaaaa
    10261 aaaagttttc atcaaaacgt cctgtttatc agttacttta attttttttt ttgattttct
    10321 ttgatatttt cttttgattt tattttgttg ttataaaaat agtc
//
LOCUS       DAORKP010000079        10307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_802_length_10307_cov_8.369684, whole genome shotgun sequence.
ACCESSION   DAORKP010000079 DAORKP010000000
VERSION     DAORKP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_802_length_10307_cov_8.369684"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..503
                     /locus_tag="PLN75_05765"
     CDS             141..503
                     /locus_tag="PLN75_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRG03656.1"
                     /translation="MENLDYNEKYRRRTKKYAVDIIRFYTQNCTKNEVMRILGKQLLR
                     SGTSVAANFRAYTRGRSIAERYSKMCIVVEEADETQFWLEIIEEAELLPFDNLSTLKN
                     ETEELVKIFTKTKASVKK"
     gene            547..1506
                     /locus_tag="PLN75_05770"
     CDS             547..1506
                     /locus_tag="PLN75_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRG03657.1"
                     /translation="MEEQVFTLSEHTDTTTFYGVNNSNILLLRNLHPNIRIVARGHVI
                     KIDGSDAEIESFKQALGRLELFTLNNNALSHENIIEIIKGNQPAEVTFDNLILHGMNG
                     KSIMARTKNQQKLVKDFETNDLLFAIGPAGSGKTYTAIALAVRALKNREKKKIILSRP
                     AVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMLPAAKLKDYMENGTIQIAPLAFMRG
                     RTLSDAVVILDEAQNSTTMQIKMFLTRMGLNTKMIVTGDITQIDLPMQQKSGLIDALE
                     VLRNVKGIAKIEFDKGDIVRHKLVQRIVEAYEKAAPRPPKGGV"
     gene            1615..2562
                     /locus_tag="PLN75_05775"
     CDS             1615..2562
                     /locus_tag="PLN75_05775"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRG03658.1"
                     /translation="MNALTKTDFHLDGQTNVYHGKVRDVYTVKNDLLVMVATDRISAF
                     DVVLPEGIPYKGQMLNQIAAQFLDATADIVPNWKLATPDPMVTVGLRCEGFPVEMIVR
                     GYLCGSAWRTYKSGVREICGVKIPDGMRENEKFPTPIVTPTTKAQQGLHDEDISKEEI
                     LKQGLATPEEYELLEKYTLALFQRGTEMAAKHGLILVDTKYEFGKRDGKIYLMDEIHT
                     PDSSRYFYAEGYEERFAKGEPQKQLSKEFVREWLMENGFQGKPGQQVPEMTPEIVKGI
                     SERYIELFENITGNRFDKSDTTQVALRIEKNITDYLNTI"
     gene            2883..5366
                     /locus_tag="PLN75_05780"
     CDS             2883..5366
                     /locus_tag="PLN75_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03659.1"
                     /translation="MKKTSITLLLAVVCILSSLAQAQNPAQIFTIKGTVVDSLSNETV
                     PFVTVSASKSTMPVMPFKRVAADAQGKFEFTVSTSEALILKFDAVGMKTTAISVSDFS
                     NKTIELGKIRIAANDKMLSEVTVIAAKPLVKVDLDKITYDMKSDPESQTSSTLEMLRK
                     VPMVTVDGDENIQVKGQSNFKIFMNGKETTMISSNPSQVLKSIPANTVKSIEVITEPG
                     AKYEAEGLAGIINIITESSLKGYTGTVRAGVDNYGAVNGGLYFSTKVGKWGITTNLNQ
                     GLFRNPGVSVETKRESFYSPDFMYFNQKNEGKNTGNFRYGSLSVSYEMDSLNLFTLSG
                     NGFGGGYNSKTTGSTNLLSQSMETVQQYNNLAEMKGIWGGYEGSIDYQRSFSKKGKLL
                     TTSYKMSYSPRGSDNNVEVENVMNYFDNKQHIKSNSSGAEHTFQLDYTEPFNKKHVIE
                     IGAKYIYRLNQSENDYQQYNFAKSAWEAMPGIVDKKMTHSNGILGAYGSYTYKLDKFS
                     ARAGGRLERTNASIQYNGDEAFTQKPFTNLVPSITLSYQTTPGSNLQLSYTQRISRPD
                     ITYLNPFRNISDPKNISQGNPDLIPEINNSFNLNYNLFTQKINFNSSIFTSFGNNSIE
                     QVSMLRDTVVYSTYENTGRSRSTGISAYVRWQVTKDFNWYFNGNTRYSYFSFTQNNKL
                     TENKGFNYGAFLGASYSLPKNFRVNTNGGYMSPSVSLQGKSNGFYYYSLSLTKSFLQR
                     KLNISANANGFLQKFVSYKGSTETDTFRSENIITRKSPRFGINVSYTFGQMKEQIKKA
                     ARGIINDDVKTGGGENATQGTQTGTQTGN"
     gene            complement(5523..5978)
                     /gene="smpB"
                     /locus_tag="PLN75_05785"
     CDS             complement(5523..5978)
                     /gene="smpB"
                     /locus_tag="PLN75_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRG03660.1"
                     /translation="MFKKSTIVIKNKRAAFDYEILDRYVAGVQLFGTEIKSIRDGKAS
                     LTETYCTFINNELWVKNMNISTYFFGTYNNHEPRRDRKLLLTKKELNKLLRATKETGN
                     TIIPIKLFVTDKGLAKLEIGLARGKKMYDKRQTLKEKDDKREMDRMMKK"
     gene            6143..6739
                     /locus_tag="PLN75_05790"
     CDS             6143..6739
                     /locus_tag="PLN75_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRG03661.1"
                     /translation="MASEIRIHIAGMIYNQSLGGTYSLVLSEDGGQKRRFSVLIGESE
                     AQSIALKLNNAKSPRPLSHDLMNSIIRELHAKVVKVVIYDLNNDIFYSEIYLIHDNKP
                     VIIDARTSDAVALAVRCDAPIYIHSDILDIVGTAIDEEKTETEQPIEEDLLRPENLNV
                     TTLKKIGTEKLKDLLEVAIEEERYEIAAMIRDEIEKRK"
     gene            6916..8187
                     /locus_tag="PLN75_05795"
     CDS             6916..8187
                     /locus_tag="PLN75_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG03662.1"
                     /translation="MKIRLIIMNFLQFFVWGAWMMTLGHYGFVEKQWNGAEFGLVFST
                     MGFASLIMPTLFGIIADKWKANYVYAILHLLFGITMCFLPLIDAPIPFFWVLFVAMSF
                     YMPTIGLNNSIGFGLLKNEGKDPTTYFPPIRVWGTIGFIAAMWITNVFTKEWGFGMSI
                     KVSFIISAIFAFVLSLFSFFFLPVLDGKKEKSAQTKKTFAQKLGLEAFILFKQKKMAL
                     FFLFSLFLGGALQLTNAYGDSFLQDATVFPKGELINNFSTIILSISQISETLFILAIP
                     FFMKRFGIKNVMLFSMIAWVLRFGLFGIAQNSIPGFIFIIASCIIYGMAFDFFNISGA
                     LFVEKNTDSKIQSSAQGVFMLMTNGVGAVIGNIVAGLVIAKWFEDPVTHVKDWQGIWF
                     TFSAYALVIAILFVIFFKYKHVKEEVADFRH"
     gene            complement(8257..9801)
                     /gene="dnaB"
                     /locus_tag="PLN75_05800"
     CDS             complement(8257..9801)
                     /gene="dnaB"
                     /locus_tag="PLN75_05800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRG03663.1"
                     /translation="MAEKRNYKKRETPTPIPANEYGKLPPQAPELEEDVLGAIMLEKD
                     AYTMISDILKPEYFYKTVHQKIFEAIIDLALQQNPIDMHTVTEMLRRKGTLDEVGGPY
                     YITLLTSRVSSAAHLIYHSQIIVQKYLARELIRLSSEIQTKAFDEKMDVDDLMQEAEG
                     KLFEITQQNLKRDVVQINPVIEEARERMKDAAKKQGASGLPSGFDALDKITSGWQKSD
                     LIIIAARPAMGKTAFILSMAKNMAVDHNSPVAIFSLEMSNVQLVNRLLMNVCNLEGEK
                     IKNGQLTPAEWERFDKDVNNLYGAPIYVDDTPSLSVFELRSKARRLVKEHNIQCIIID
                     YLQLMNASGLSFGSREQEVSLISRSLKGLAKELDLPIIALSQLNRGVEGRTGIDGKRP
                     QLSDLRESGAIEQDADMVCFIHRPEYYGIPADSQGNSYEGIAEIIISKHRNGAVGDVK
                     LKFKKTYAKFLNLTDENPFGDGNFAVISSKMNTDFESETPPEYLQTAPLPEYIQTPSS
                     PGDVPF"
     gene            9956..>10307
                     /locus_tag="PLN75_05805"
     CDS             9956..>10307
                     /locus_tag="PLN75_05805"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRG03664.1"
                     /translation="MTVYPNAKINLGLNVVERRSDGYHNIETIFYPIGIQDVLHVEHS
                     EMCSDYSFSSSGISVGGDPEMNLIIRAFRLLRSHYQFPPVDITLHKQIPFGAGLGGGS
                     SDAAFMLKALNELFE"
BASE COUNT         3148 a         2181 c         2175 g         2803 t
ORIGIN      
        1 ggtaaaagac cgggtgaaca aaacaatgag caactatccg ttgtttgcgt attgaaagag
       61 gcaaggagta agaggtaaga ggtatgctgc tctaaattaa tcacttatta tttttataat
      121 taactaaata tgtttttcac atggaaaatt tagattataa cgaaaaatac agaagaagaa
      181 caaaaaaata tgctgtggac ataatccgct tttatacgca aaattgcaca aaaaatgaag
      241 ttatgaggat tcttggcaaa caattattgc gttcaggaac ttctgttgct gctaatttca
      301 gagcatatac tcgtggacgt tcaattgctg aacggtattc aaaaatgtgt attgttgttg
      361 aagaggcaga tgaaactcaa ttctggttag aaataataga agaagctgaa cttctacctt
      421 ttgacaactt atcaactctt aaaaacgaaa cagaagaatt agttaagata tttacaaaaa
      481 caaaagcaag tgttaagaag taagggactt acctcttact ccttacctct tgcctctcaa
      541 aatactatgg aggaacaagt tttcaccctt tccgaacaca cagatacgac cacattttac
      601 ggagtaaaca acagcaacat cctgctgctg agaaatttac atcccaacat ccggatagtg
      661 gcccgcggac acgtgataaa aattgacggc agcgatgccg aaattgagag tttcaagcaa
      721 gcgcttggca ggttggagct tttcactttg aataacaatg ctttatcgca cgaaaatatc
      781 atcgaaatta taaaaggcaa tcaaccggct gaggttactt ttgacaattt gatactgcac
      841 ggtatgaatg gcaagtccat catggcacgt accaaaaatc aacaaaagct ggtgaaggat
      901 tttgaaacaa acgatttgct ttttgctatc ggacccgccg gatcgggtaa aacctataca
      961 gccattgcgc tggcggtgag agctttgaaa aatcgcgaaa aaaagaaaat cattctgagc
     1021 cgtccggcgg tggaagcggg tgaaaaactg ggttttctgc ccggcgatat gaaggaaaaa
     1081 attgatccgt atctgcaacc actttacgat gcacttcagg acatgctgcc tgccgccaaa
     1141 ctgaaagatt acatggaaaa cggcaccatt cagattgcac cgctggcatt tatgcgcgga
     1201 cgaacactga gcgacgctgt ggtaattctc gacgaggcac aaaactccac cactatgcag
     1261 ataaaaatgt ttctgacacg catggggctg aataccaaaa tgatagtaac cggtgacatt
     1321 acacaaatcg acctgccgat gcaacaaaaa tcaggattga tagatgcact ggaagtgcta
     1381 cgaaacgtca aaggaattgc aaaaattgaa tttgacaaag gggatattgt ccgtcacaag
     1441 ttggttcaac ggatagtaga ggcgtatgag aaagctgccc cccgtccccc taaaggggga
     1501 gtttaatccc atatcaaatt gttttttata caaaatagat attcaaaaac aaaatcaatc
     1561 taatttaatg ctttccaatt ccccctttag ggggtaaggg ggcaaataca tattatgaac
     1621 gcacttacaa aaacagactt ccatttagat ggacaaacaa acgtgtatca cggcaaagta
     1681 cgggatgtgt acaccgtgaa aaatgattta ttggtgatgg ttgctaccga ccgcatttcg
     1741 gcttttgacg tggtgctgcc cgaaggtatt ccgtacaaag gacagatgct caaccagata
     1801 gcagctcagt ttctggatgc taccgctgat atagtaccca actggaaact tgccacaccc
     1861 gatccgatgg tcaccgtggg acttcgctgc gaaggttttc cggtggaaat gattgtacgc
     1921 ggttacctct gcggaagtgc ctggagaacc tacaaaagcg gcgtacgtga gatttgcgga
     1981 gtaaaaattc cggatggaat gcgcgaaaac gagaagttcc ctactccaat cgtaacaccg
     2041 accacgaagg ctcagcaagg acttcatgat gaagacattt caaaagaaga aattctgaaa
     2101 caaggtcttg caacacccga agaatacgaa ctgctggaaa aatatacgct tgcccttttc
     2161 caacgcggta cggaaatggc agccaaacac gggctgattt tggtggatac caaatatgaa
     2221 ttcggaaaac gtgacggcaa aatttacctg atggacgaaa tccacacgcc cgacagttcg
     2281 cgatacttct atgccgaagg ctacgaagaa cgttttgcca aaggagaacc gcagaagcag
     2341 ctttccaaag aatttgtacg tgaatggctg atggagaatg gattccaggg aaaacccggg
     2401 cagcaggtgc ccgaaatgac tcccgaaata gtgaaaggca taagcgaacg atacatcgaa
     2461 ctgttcgaaa atatcaccgg aaaccgcttc gataagtctg atacgactca ggtagcactg
     2521 cgcatagaaa aaaacataac ggattacctg aatacaatct aaaaaaatca gttcaaaact
     2581 acaaattaaa ctgtcagtgt cgtgtttaac gaattctgac agtttttttt ccacaatcct
     2641 tccatatacg gctttctggc aataaaaaaa cacacgtagt aagtaaagtt tttatgttaa
     2701 acggatgttt ttccggcaat atgcctatat acgctgaaac atccactgag aaaaggtttg
     2761 aagcaggata ccattaacat aattttgcat tattaattat gtttaatttc aaataaaaac
     2821 attttttcaa aacccatctt tctatgcatt tacactactt ttgtgatcaa attaacataa
     2881 ttatgaaaaa aacttctatt accctgctac tcgcagttgt atgtatttta tcctcattgg
     2941 cgcaggcaca gaatcctgcc caaattttca ccattaaagg aaccgttgtt gattctcttt
     3001 caaacgaaac ggttcccttc gtcaccgttt ctgcatcaaa atctaccatg cctgtcatgc
     3061 catttaagcg ggtagccgcc gatgctcagg gaaagtttga attcactgtc agcacatcgg
     3121 aagcgcttat tcttaaattc gatgcagtag gaatgaaaac gacagccatt tccgtgtccg
     3181 atttttcaaa caaaacgata gaactgggca aaatcaggat agcagccaac gacaaaatgc
     3241 tctcagaagt aactgtaata gccgcaaaac ctttggtgaa agttgatttg gataaaatca
     3301 cctacgacat gaaatcggat ccggagtcgc aaacgtccag tacgctcgaa atgctgcgaa
     3361 aagtgcccat ggtaaccgta gatggtgatg aaaacatcca ggtgaaagga cagtcgaact
     3421 tcaagatttt catgaacgga aaagaaacca cgatgatttc gagcaacccc tcgcaggtgc
     3481 tcaaaagcat ccctgccaac acggtgaaaa gcatcgaagt tatcaccgaa ccgggtgcaa
     3541 agtacgaagc agaaggcctg gccggaatca ttaatatcat caccgaaagt tcactgaaag
     3601 gctatacagg taccgtgaga gccggagtag ataactacgg agcagtcaac ggcggccttt
     3661 atttttcgac caaagtagga aaatggggta tcactaccaa tctgaatcag ggtttgttcc
     3721 ggaatccggg cgtctctgtc gaaacgaaaa gagaaagctt ttattcaccc gatttcatgt
     3781 atttcaatca aaaaaacgaa gggaaaaata ccggaaattt tcgttacgga agtctgagtg
     3841 tgagctacga gatggattcg cttaaccttt tcacgctgag cggaaacgga ttcggaggtg
     3901 gctacaacag taaaaccacg ggaagcacca accttctctc tcaatccatg gaaacggttc
     3961 agcaatacaa caatttagca gaaatgaaag gaatctgggg cggatacgaa gggagcatcg
     4021 actaccagcg ctcattcagt aagaaaggaa aactgctgac cacttcgtat aaaatgagtt
     4081 attcaccccg cggaagcgat aataatgttg aagttgagaa tgtgatgaat tattttgaca
     4141 acaagcagca catcaaaagt aattcatcgg gcgccgaaca cactttccag cttgactata
     4201 ccgaaccatt caacaaaaaa catgtgattg aaataggggc aaaatacatt taccgtctca
     4261 atcagagtga aaacgactat cagcagtaca atttcgcaaa aagcgcttgg gaggcaatgc
     4321 cgggcattgt tgacaaaaag atgacacact ccaacgggat tctgggtgcg tacggaagct
     4381 acacctacaa acttgataaa ttcagtgcac gtgccggcgg acgactcgaa cgaaccaacg
     4441 catccattca atacaacggc gatgaagcgt tcacccaaaa accgttcacc aatctggtac
     4501 cttctatcac actgagctat cagactacgc cgggatccaa cctgcaactt agttatacgc
     4561 agcgtatcag ccgccctgac attacgtatt tgaatccgtt cagaaatatc tccgatccga
     4621 aaaacatatc gcagggaaat ccggacctga tacctgaaat caataactcc ttcaacctga
     4681 attacaattt atttactcag aaaatcaatt ttaattcgag catattcacc tctttcggca
     4741 ataattccat cgagcaggtg agcatgctga gggatacggt ggtttactcc acgtacgaaa
     4801 acaccggccg ctcacgcagt acaggtattt ctgcttatgt aaggtggcag gtcaccaaag
     4861 atttcaactg gtatttcaac ggaaacaccc gatactcata cttttcgttc acacaaaaca
     4921 ataaactgac cgaaaacaaa ggattcaact acggagcatt tttaggcgca agctattcgc
     4981 tgccaaaaaa cttcagagta aacactaacg gcggctatat gtcgccctcg gtttcgctgc
     5041 agggaaaatc caacggtttt tactactaca gtttgtcgct taccaaaagc tttttgcaac
     5101 gaaaactcaa catctcggcc aacgccaacg gttttctcca aaaatttgtc agttacaaag
     5161 gctcaactga aaccgacact ttcagaagtg aaaacattat cacgcggaaa tcaccccggt
     5221 ttggtatcaa cgtgagctat actttcgggc agatgaagga acagataaaa aaagcagctc
     5281 gtggtattat caacgacgat gtgaaaaccg gcggaggcga aaatgctact cagggcacac
     5341 aaacaggcac acaaacaggc aattaactcc ttcgtagtta atcacttaca ggtgtaaaaa
     5401 aaaaggttcg gtgaatgcct gaaatggcaa atcaccgaac tttttttttc ttttttgaat
     5461 ttttaaaagg agaaacatca gaaaaaaaaa ttcagaaaaa aaaatccccc gaaaatgaat
     5521 gattattttt tcatcatccg gtccatctcg cgtttatcgt ctttttcttt cagcgtctgc
     5581 cgcttgtcat acattttttt accgcgtgcc agacctattt caagttttgc aagtccttta
     5641 tcggttacaa acaatttgat gggaataatc gtattgcccg tttcttttgt tgcccggagc
     5701 agtttgttga gctctttttt ggtgagcagt agtttacggt cgcgccgggg ctcgtgattg
     5761 ttgtatgtac cgaaaaaata agtggatata ttcatgtttt tcacccacag ttcgttgttg
     5821 ataaaagtgc agtaagtctc tgtaagagaa gcttttccgt cgcgtatcga cttaatttca
     5881 gtgccgaata actgaacgcc tgctacgtaa cggtcaagga tttcataatc gaatgcagcg
     5941 cgtttgtttt ttataactat ggttgatttc ttaaacatcc tgtttatttt acaatttccg
     6001 gcaaatttaa gcataagttg gaaataattg aaaaaagaga tgaaagttgc tgcaataaat
     6061 aggttggatg gactattttt aatcccaaaa tgaacagatt gtataaaaaa atgcttattt
     6121 ttgcttaaat ttttatcaga aaatggcatc agaaatcaga atacatattg caggaatgat
     6181 ctacaaccaa tcgctgggag gtacctattc gttggttttg agtgaagacg gcgggcagaa
     6241 aagacggttt tcggttttga ttggcgaatc tgaagcacaa tccattgccc tgaaactgaa
     6301 caatgccaag tctcctcgtc cgctttcgca cgatttgatg aactcgatca tccgcgagct
     6361 gcatgcaaaa gtggtcaaag tggtcatcta cgacctgaac aacgatattt tttactctga
     6421 gatttacctc atacatgaca ataagcctgt aatcatcgac gcacgcactt cggatgctgt
     6481 agcgctggct gtgcgctgcg acgcacccat ttacattcat tcggatatac tggatatagt
     6541 aggaactgcc atcgacgaag aaaaaaccga aaccgagcaa ccaatagagg aagatctgct
     6601 acgaccggaa aacctgaacg taactacatt gaagaaaatc gggacagaaa agctgaaaga
     6661 tctgcttgag gtggctatcg aagaagaaag atatgaaata gcagccatga tccgcgatga
     6721 aattgaaaaa cgaaagtaga gaaatagttc ggagttttga gttcggagtt ttgagttctg
     6781 atttctgact tctgacttca gtgttggctt tttggaaact gcatgccgac tgtatttaat
     6841 cgtttttcaa aaaaaaacaa ctggaaagta aaaatacaaa aataaaatca gataaattta
     6901 ccaaaaaaca caaacatgaa gattcgactt attatcatga attttctcca gttcttcgta
     6961 tggggagcct ggatgatgac ccttgggcat tatggatttg tagaaaaaca atggaacgga
     7021 gctgagtttg gcttagtatt ttccaccatg ggttttgcat cgctcattat gcctactctg
     7081 tttggcatca tagccgacaa atggaaagca aactacgtgt atgcaatact gcatttgctg
     7141 tttggaatta ctatgtgctt tcttccgctg attgacgcac ccattccgtt tttctgggtt
     7201 ctctttgttg ccatgtcttt ttacatgcct accatcgggt tgaacaactc catcggtttt
     7261 ggattattaa aaaatgaagg aaaggatcct acaacgtact ttccgcccat ccgcgtgtgg
     7321 ggaactattg gatttattgc agccatgtgg atcaccaatg tattcaccaa ggaatggggc
     7381 ttcggtatga gcattaaagt atcgtttatc atttctgcta tctttgcttt cgttctatcg
     7441 ctgttttcgt tcttttttct tcctgtactc gacggtaaaa aggaaaaatc agcccaaact
     7501 aaaaaaacat ttgcccaaaa actcggactg gaagcattta ttttattcaa acagaagaaa
     7561 atggcgcttt tctttctttt cagtctattt ctcgggggag cattgcaact caccaatgcc
     7621 tacggcgaca gttttctgca ggatgctacc gtatttccaa aaggtgaact cattaataat
     7681 ttctcgacca tcatcctgtc catttcgcag atttccgaaa ccttgttcat tctggccatt
     7741 ccatttttta tgaaacgatt tggtatcaaa aatgtaatgc ttttcagcat gatagcgtgg
     7801 gtacttcgtt tcggcttgtt cggaattgct cagaattcta tacccggatt tatatttatc
     7861 attgcatcgt gtatcattta cggaatggct ttcgatttct tcaatatctc aggagcattg
     7921 tttgtcgaaa aaaataccga ttcaaaaatt cagtcgtcgg ctcagggagt attcatgctg
     7981 atgaccaacg gtgtaggagc tgtgataggg aatatagttg ccggactggt gatagccaaa
     8041 tggtttgaag acccggtgac acatgtgaaa gactggcagg gcatctggtt taccttttct
     8101 gcctatgcgc ttgttatagc cattcttttc gtcattttct tcaaatacaa gcatgtgaaa
     8161 gaagaagttg ccgatttcag gcattgaaca ttcgctttat ttcacactat aatattaaag
     8221 catcaaaccg gccggtttga tgctttttat ttattatcag aaaggaacat cgcccggaga
     8281 ggacggtgtt tgaatatact caggcaaagg tgctgtctgc aaatattcag gtggcgtttc
     8341 gctttcgaaa tcagtattca tttttgagga aataacggca aaattaccat caccaaacgg
     8401 attttcatcg gtcagattga ggaatttggc atacgttttc ttgaatttaa gctttacatc
     8461 tcctaccgcg ccgtttcggt gtttcgaaat aatgatttcg gcaattccct cgtacgaatt
     8521 tccctgagaa tctgcaggta tgccataata ttcgggacgg tgaatgaagc aaaccatatc
     8581 ggcatcctgc tcgatggctc ccgactcgcg caaatccgaa agctgtggtc gtttgccatc
     8641 aattccggta cgtccttcta caccacggtt caactgcgac agggcaatga tgggcagatc
     8701 aagctctttg gcgagtcctt tgagcgaccg ggatatgagg ctcacttcct gctcgcggct
     8761 gccgaaagag agcccgctgg cattcataag ctgcaggtaa tcaatgataa tgcattgaat
     8821 attatgttct tttaccaggc ggcgggcttt acttcgcaac tcgaaaacag acagactcgg
     8881 cgtgtcatcc acataaatgg gtgcaccgta caggttgttt acatccttgt caaatcgttc
     8941 ccattcggcg ggagtgagct gaccgttttt aattttctca ccttcgaggt tgcacacatt
     9001 catcaacaaa cggttgacta actgcacatt ggacatttcc aatgaaaata tagccaccgg
     9061 agaattgtgg tcaacggcca tgtttttagc catggaaagt atgaatgccg ttttccccat
     9121 tgccggacgg gctgcaatga ttattaaatc ggacttttgc cagccggatg taatcttatc
     9181 cagcgcatcg aatcctgacg gcagaccgct tgctccctgc tttttagctg catctttcat
     9241 tctttcgcgg gcttcttcta tcacaggatt aatctgcacg acgtcccttt tcagattttg
     9301 ctgcgtaatt tcaaaaagtt tgccttcggc ttcctgcatc aggtcgtcca cgtccatttt
     9361 ttcgtcaaaa gccttggtct gaatttcgct tgaaagccgt ataagctctc tggcaaggta
     9421 tttctgaaca ataatctgtg agtggtaaat aaggtgggct gccgacgaaa cgcgcgaagt
     9481 cagcagtgtg atgtagtaag gtcctcccac ctcgtcgagc gtaccttttc ggcgcagcat
     9541 ttcggtcacg gtgtgcatat caatcggatt ttgctgcaaa gccaggtcaa tgattgcttc
     9601 aaaaattttc tgatgaacgg ttttatagaa atattccggt ttcagaatat ccgatatcat
     9661 ggtgtaagca tccttctcga gcatgatggc tccgagcaca tcttcttcaa gctcaggtgc
     9721 ctgtggcggt agttttccgt attcattggc cgggataggc gttggagttt ctcttttttt
     9781 gtaattacgt ttttctgcca tttttaactt tttgttttgg gtctcaaagt tacgaaaaga
     9841 ataagggatg taaaatgaaa aaaaataatt ctttcaacaa gtaatcaaca gttgattaca
     9901 gctttgaaat acaaaaaaaa tacggtaatt ttgacaaaaa ataaactaaa aaacaatgac
     9961 agtatatcct aacgcaaaaa taaatctggg tctgaatgtg gtggaacgcc gttcggacgg
    10021 ctaccacaac atcgaaacca ttttttaccc catcgggatt caggatgtac tgcatgtaga
    10081 acattccgaa atgtgcagcg attacagttt ttcatcgtcg ggcatttcag tgggtggcga
    10141 tcctgagatg aatcttatta tcagggcatt tcgtctactc cgttctcatt atcagtttcc
    10201 acccgtagat atcacactcc ataagcaaat tccattcgga gcaggactcg gtggtggttc
    10261 gtcggacgct gccttcatgc tgaaagctct gaatgaactg ttcgaac
//
LOCUS       DAORKP010000080        10175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_824_length_10175_cov_8.467589, whole genome shotgun sequence.
ACCESSION   DAORKP010000080 DAORKP010000000
VERSION     DAORKP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_10175_cov_8.467589"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PLN75_05810"
     CDS             complement(<1..1262)
                     /locus_tag="PLN75_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HRG03665.1"
                     /translation="MKSKFHIACLLIITSAFISCASARSAYKYESLYENLAFSMPKVQ
                     APVFPDNRVNLPDVGGKPDGISLNTEAFEKAMKSLSDKGGGTLIVPKGIWFTGPIVFR
                     SNINMHLEKGALILFSPDFNLYPLVETVFEGLDTRRCQSPISGRNLQNVAITGQGSID
                     GSGDAWRPLKKEKVTERMWKQKVASGGVVRSGNYWFPTAASLKGHEMSDMNVPRKDMT
                     ETQWLEIKDFLRPVMISFIECKNVLLEGVLFENSPAWNIHPLMCENVIIDGIYVKNPS
                     YSQNGDGLDLESCVNSIVVNSTFDVGDDAICIKSGKDEDGRRRGRPTVNLIVDNCKVF
                     HGHGGFIVGSEMSGGVRNVSVRNCQFLGTDVGLRFKSNRGRGGVVENIYISDIYMFNI
                     ATDSFLFDLYYGGKSASESLADGDVTPVA"
     gene            complement(1397..3043)
                     /locus_tag="PLN75_05815"
     CDS             complement(1397..3043)
                     /locus_tag="PLN75_05815"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HRG03666.1"
                     /translation="MKKNMNIAKRILLPVVMLLVLAGCDRTEDLGNPDRLFRPIFKDP
                     VVSMTWVKAEWDKYKGTNSYEVELSTDTFKNILRSNRTDSARITFNNLEFDTPYQIRV
                     RGVGDSILSSGDTIRSEYNVLHVRTQDYPTQLITPASADVIDKSIRVKWKVTSLVYSH
                     IDVMLDKYTVYKTVPLTEADNLAGEKIISGLNPATSYIVKIFSEDGYKGKKSFKTVAS
                     QVFEGPVVDLRDFADDVSFSKITQAFIDSLGTQYPEGFNLVLAGGTRYKLPTINIPVS
                     MNMVTGLSFKGKAIVAVNGSFGIKGATKVNSVKIEKVFFTEGTDAGKLKTDANFGGTY
                     LFNFNQAEGNLGTLTVENCDIKYKRGVVRLQTTAKIDLLTINNTVADSIGGYGIVNLD
                     NAASMVTDLVIKNSTFSHFDGYICRNIKSTASPNSIYVENITTCYAPASTRYFFELTD
                     RAYPGGITIKKSIFGSVLAAGTTVHGLRSASTNVTVENCFKTSDLVWTIAAGATEPTY
                     PIECTDLGKTSAEIFANPASGNFRVTSSALVKKAGDPRWW"
     gene            complement(3095..5053)
                     /locus_tag="PLN75_05820"
     CDS             complement(3095..5053)
                     /locus_tag="PLN75_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03667.1"
                     /translation="MKIKQYLTVLIAAVFTLSSCSDFLEPVSESNFDETYVFSNAGDA
                     KKMVMGVYALFTQDSYTSRMSNVWMQNTDVEVQAPSALPDGSRRDVWSLQGGLLTGFG
                     DIKTAWDQNYLAIDRANQCIEGIQKSPEAKTGEMKMLLGESYCLRAYRYFLLINFWGD
                     VPYFRDAANATMQLDVPKTDKNIIYSGMIQDLVNSEADMFFADEFSDGIERMNREFAL
                     GMISRLALFRAGYGMTKGGEMKRADEYLNIQTNDSLAVTYTYKGVTKTARTSTEYFQL
                     AKDYAEKLTELKPRQLTDFATVFMNQNKWIKPVNSDILYEVAFGNTNSGGDVGWCVGV
                     PVTGGSYGKTTIQVGFSPTYYFMFDEKDKRRDVTISKVSYTDENTQSTLGVTSLAAGK
                     WNRIFLPAAPGEASSKGTGINWPLMRYSDVLLMLAEAENELNGPTALARNLLKTVRAR
                     AFDAADHALQVENYVNSLTDKTAFFDAIVTERALEFGGEGLRKFDLVRWNIYGKKIVE
                     TKTALDNMGKAGNNLELENPEVAKYAGYARKLYYQKRSGKIKWYNTFYNPEVIPEMIV
                     DDADLEKAGNENAYATINWTQSLYKKVTDTEGNVTYTTADFTDRCWRGYKDPSGITAV
                     PYLLPINIQTVAASKYLNNEGYGLVLTN"
     gene            complement(5089..8361)
                     /locus_tag="PLN75_05825"
     CDS             complement(5089..8361)
                     /locus_tag="PLN75_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03668.1"
                     /translation="MMQKHKSRAKGFALFLGMMLMASGLLAQQRNISGTVKDVSGEPI
                     IGASVLVKGTNSGVITNIDGEYNLSVPGSATTLTVRYVGKTPKDVAITGNVIHVVLED
                     DQKVLDEVVVIGYGTARRRDLTGSVTSIQGKTIAQIPVTGTAQAMAGRMAGVQVTSTD
                     GSPDAEILVRVRGGGSITGDNSPLYIVDGFPASNINDISPSDIQSIDVLKDASSTAIY
                     GSQGANGVVIVTTKQAQGGKTKVSYNGFVQTKRLSKRLEVLDPFEYALLNYEYGALGG
                     EDGIKSYEKTFGVFEDIDLYKYMRAHDWQQDMFGSDVVSQQQNISLTGGTDKTKFSLS
                     STFNKDGGLMTGNDYTRFTTNFKLDHELYKNLKATFNLRLGDTKVNGSGSSGGLYKIR
                     TTQAVTSPAVKGLSEMIVVDPALMTEDEYEQWQRSNMSLTEQAQQYWKRRNDRTFNFI
                     GGLDWKIIKSLVYRIEGGYEYGFGEVKNYWGENTTNASYVDGKPLVDWTKTNSNKLRL
                     ANTLTYMQTFAGDHSINVLAGQELISNTANNTYIYATGYSPDLTPEKIFANLALGGAS
                     KDIRSTVSIPNNLLSFFGRAVYNYKERYLLSATFRADGSSRFAPGNQWGYFPSVAAAW
                     RIVEEPFMKNTKDWISNLKLRYSYGEAGNNKIGGGQFQKMYSIQSTKTYGLGDIQNNY
                     WATSNTQLPNKDLRWETSVTQNIGLDFGFFDEKLSGTFELYQNTNKDLLLEIPIVAPG
                     YRTTFRNIGQTTNKGIELSLNSVFAQRKNFSLYGNFNIGFNKSNVDKLADGITLQEYA
                     SGWAGTDLKGYYDYQVKVGEPVGLIYGWVNDGYYKTTDFTFDPASNKYTLIQGVPSIG
                     MLGGRIGTRPGTAKFKDLSGPNGQPDGVVNDFDRTVIGRTAPKFLGGFGLNGTVYDFD
                     FTMLFNFVYGNQVYNANKIASAQQYRTTNPNMLAFMRQNNRYTYLNNETGEIVYDLET
                     LAQMNEGANAKEYWSPFSFGNAVAVPMSWAIEDGSFLRFQNLTVGYTVPKMLSNRIGM
                     DQLRVYATVNNLWILTRYTGYDPEVSTAVRGSSTSGLTPGVDYSSYPKSLSWTFGLNL
                     TF"
     gene            8808..9896
                     /locus_tag="PLN75_05830"
     CDS             8808..9896
                     /locus_tag="PLN75_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRG03669.1"
                     /translation="MQKRIRIKDIARMAGVSAGTVDRIIHGRGSVSKKSKEVVESILR
                     EVNYRPNIHMSSISLKKTYNILFVAPGFAEGEYWESIYKGIQRALNVFENISVNCSIM
                     TYNQYDVYACREVFESALEPDFDAVIIGPIFQHETREFCRKLKMKNIPYVFVDSNVED
                     TSPLAYFSANHYISGYLMCRLIHSIMQNGKDIGIMQAMRVGNESANSTILRKNGFSDY
                     QKENRLKNKIHRIPFSATEPESNEQLFNDFFSENDNIGGIVVLNSRGYVIANYLHKKK
                     IKDMKVVCVDLTDANKKALKKGHIDFLIGQKPERQGYLAMKFLIEHLIYKIPVKVANI
                     LPLDILTRETIDLYSEFNDLVYLDQYEK"
BASE COUNT         2595 a         2312 c         2268 g         3000 t
ORIGIN      
        1 gctacgggcg tcacatcgcc atcggcaagc gattcggaag ctgattttcc gccgtaatac
       61 agatcgaaaa ggaaagaatc ggtagcgatg ttaaacatat aaatatctga tatatagatg
      121 ttttcaacta cgccgcctct acctctgttg ctcttgaaac ggaggcctac atcggtgccc
      181 agaaactggc agtttctcac agatacattt cgcacaccgc ccgacatctc gctgcctacg
      241 ataaatccgc cgtgtccgtg gaatacttta caattatcca caatcaggtt gactgtagga
      301 cgtccacggc ggcggccgtc ttcatcctta ccggatttga tgcagatggc atcgtcaccc
      361 acgtcgaaag tgctgttaac cacaatggaa ttgacacacg attcgaggtc gagtccgtcg
      421 ccattctgtg aatagcttgg atttttcacg taaataccgt cgataatcac attttcacac
      481 atcagcgggt ggatattcca ggcgggtgaa ttttcaaaca gaacgccttc aagcagtaca
      541 tttttacatt ctataaaact aatcattacc ggacgaagaa aatctttaat ttccagccat
      601 tgagtttcag tcatatcctt gcgcggcacg ttcatatcgc tcatttcgtg tcctttaagc
      661 gatgcggctg ttggaaacca gtaatttccg ctgcgtacca cacctcccga cgctactttc
      721 tgtttccaca tcctttcggt taccttttcc tttttgagag gcctccaggc atcgcccgat
      781 ccatcgatgg agccttgtcc ggtaatggca acattctgca gatttcttcc cgaaatgggc
      841 gactggcagc gacgtgtatc gagcccttcg aaaacggttt cgaccagcgg atagaggtta
      901 aaatcgggtg aaaaaagtat cagagctcct ttttcgaggt gcatgttgat attcgaacgg
      961 aaaacaatgg gtccggtaaa ccagattcct ttcggaacaa ttaaagttcc tcccccttta
     1021 tccgaaagac tcttcattgc tttttcgaag gcttcggtat tgagggagat gccgtcgggt
     1081 tttcctccca cgtcgggcag atttacacgg ttatcgggga atacaggtgc ctgcactttg
     1141 ggcatgctga aagcgagatt ttcatacaat gattcatact tgtaagcact ccgcgcagaa
     1201 gcacaggaga tgaatgccga tgtgataatg agcagacagg ctatatggaa ttttgatttc
     1261 atggatattt ccggtatatt tttcattctg tattttccgt tcattcatac tttcagaaaa
     1321 gcatgattca ggatttgttt cgtaataaaa aagcaggttt taccctactt ttcagtaagg
     1381 taaaaccttt agaatattac caccagcggg gatcgccggc ttttttcacc aacgcgctgc
     1441 tggtaaccct gaagttaccg gatgcaggat ttgcaaatat ctcggcggat gttttgccaa
     1501 gatcggtaca ttcgatagga tacgtgggtt cggtagcacc ggcagctata gtccatacca
     1561 gatccgaagt cttgaagcag ttttccaccg ttacattggt ggaagccgaa cgcaaaccat
     1621 gcacagttgt tcctgctgca agcacactgc cgaaaattga ttttttgatt gttataccac
     1681 ccggataggc tctgtcggtg agttcgaaga aatatcttgt cgatgccgga gcgtagcagg
     1741 tagtgatgtt ttctacgtaa atggaatttg gtgatgcagt agatttaata ttgcggcata
     1801 tgtaaccatc aaaatgcgag aaagtggaat ttttaatcac cagatcggtt accatacttg
     1861 ctgcattatc caggttcaca attccataac cgccaattga atctgcaacg gtattattga
     1921 tggtcaaaag gtcaattttg gcagtggtct gcagacgcac cacacctctt ttgtatttga
     1981 tatcgcaatt ctcaactgtc aatgttccaa gattaccctc tgcctgattg aaattgaata
     2041 aataggttcc tccaaaattg gcatcggttt tgagttttcc ggcatcggtt ccttcggtaa
     2101 aaaatacttt ttcaattttc acagaattaa cctttgtagc gcctttgata ccgaagcttc
     2161 cattaacggc aactatggct ttacccttga aacttaagcc ggtaaccata ttcatggata
     2221 caggaatgtt gatggtaggc agtttgtatc gggtacctcc cgccagcaca aggttgaaac
     2281 cttcgggata ttgggtgcca aggctgtcga taaatgcctg ggttattttg ctgaaagata
     2341 catcgtcggc gaaatcgcgc aagtcaacca ccggcccttc gaatacctgc gaagcaacgg
     2401 ttttgaatga tttcttgcct ttgtatccgt cttcggaaaa tatttttaca atatacgagg
     2461 tagcaggatt tagacctgaa atgattttct caccggccag attatccgct tcggtcaggg
     2521 gtacggtttt gtaaaccgta tatttatcca gcattacatc tatatgactg taaaccaatg
     2581 atgtaacttt ccattttacg cggatggatt tgtcgataac atcggccgat gcaggtgtaa
     2641 taagctgagt cggatagtcc tgcgtacgta catgaagtac gttatattcc gaacgaatcg
     2701 tatcgcccga agataaaatg gagtcgccca ctccgcgaac cctgatttga tacggtgtat
     2761 caaactccag attattgaaa gtgatgcgtg cactgtcggt acgattcgac cggagtatgt
     2821 ttttgaacgt gtcggtcgaa agttcaactt cgtacgaatt agtccctttg tatttatccc
     2881 attcagcttt tacccaggtc atactcacaa ccgggtcttt gaaaatcgga cggaacagtc
     2941 tgtcgggatt tcctaaatct tccgttcgat cgcaaccggc caaaaccagt aacataacca
     3001 ccggtaacag gattcttttt gcaatattca tatttttttt catatttttt tttatttttt
     3061 ctgataaaaa tagagcagtc ggttttataa aaaatcaatt ggttaacacc agaccgtagc
     3121 cctcgttgtt caggtattta cttgcagcta ctgtttgtat gttgatcgga agcagataag
     3181 gaactgccgt aattccggag ggatctttgt atcctctcca gcatcggtcg gtaaaatcgg
     3241 ctgtagtata agttacattt ccttcggtat cagttacttt tttatagagc gactgtgtcc
     3301 agttgattgt tgcatacgca ttttcgtttc cggctttttc caggtcagca tcgtccacta
     3361 tcatttcggg tatcacttcc ggattgtaga aagtattgta ccattttatt ttaccgctcc
     3421 gtttctggta ataaagcttt ctggcgtacc cggcatattt ggccacttcg ggattttcaa
     3481 gctccaggtt attgccggct ttacccatat tgtcgagtgc ggtttttgtt tccactatct
     3541 ttttaccgta gatattccag cgcaccaggt cgaatttgcg taaaccctcg ccaccgaatt
     3601 caagtgcacg ttcggtgaca atggcgtcga agaaagccgt tttgtccgta agggagttta
     3661 catagttttc cacctgtaag gcatgatcgg cggcatcaaa agctcttgcc cgaacggttt
     3721 taagtaaatt tctggccagt gctgtgggtc cgttcagttc attttctgct tcggccagca
     3781 tgagcagcac atcggaataa cgcatcagcg gccagtttat gcccgttcct ttggatgagg
     3841 cttcacccgg tgctgcaggg aggaaaatgc ggttccattt tccggcagca agactggtta
     3901 cacccaacgt gctttgcgtg ttttcatctg tatagctcac tttcgatatg gttacatcgc
     3961 gtcgtttgtc tttctcgtcg aacataaaat aataggtggg tgaaaatcct acctgaatgg
     4021 tcgtttttcc atacgaacca ccggtaacag gcacacctac gcaccatccc acatcgccgc
     4081 cgctgttggt attgccaaag gctacttcat agagaatatc actgtttaca ggttttatcc
     4141 acttgttctg attcataaat acggtggcaa aatcggtcag ctgccgcggc ttcagctctg
     4201 tgagtttttc ggcataatct ttggccagct gaaaatattc tgtcgatgta cgggcagttt
     4261 ttgttactcc cttgtaggtg tatgttacag ccaggctgtc gtttgtctga atattgaggt
     4321 attcgtcggc gcgtttcatt tcaccgcctt tggtcattcc gtagccggct ctgaagagtg
     4381 ccagacgcga aatcattccc agtgcaaact cgcggttcat gcgttcaatg ccatcgctga
     4441 attcatcggc aaagaacata tcggcttcgc tgttcaccag atcctgaatc atgcccgaat
     4501 aaatgatgtt tttatccgtt ttaggcacat cgagctgcat ggtggcattc gcagcatcgc
     4561 gaaaataagg cacatcgccc cagaaattga taaggagaaa atagcggtaa gctctcaggc
     4621 aatagctttc gccgaggagc attttcattt ctccggtttt tgcttcaggg cttttctgga
     4681 taccttcaat gcattgattg gcacgatcga tggccagata attttgatcc caggcggttt
     4741 tgatatcgcc gaaacctgta agcagcccgc cctgcagtga ccatacatcg cggcgcgaac
     4801 catcgggcaa ggcactggga gcctgaactt ccacatcggt gttttgcatc cacacgttgc
     4861 tcatacgcga tgtgtatgaa tcctgtgtga aaagtgcata aacacccatt accatttttt
     4921 tggcatcgcc ggcattcgaa aacacgtaag tttcatcgaa atttgattcc gacaccggtt
     4981 ccagaaaatc gctgcacgaa ctaagggtga atacggcagc tatcagtact gttaaatatt
     5041 gcttgatttt cattgtttgt ctgtttttaa attttcggtt taacatggtc agaatgtaag
     5101 attaagtcca aaagtccacg ataagctctt tggataggag gaatagtcca caccgggtgt
     5161 aagtcctgaa gttgaacttc ctcttacggc agtgctcact tcggggtcgt agccggtgta
     5221 cctggtgagt atccacaggt tattcacagt agcatatacc cttagctgat ccattccgat
     5281 cctgttgctc aacattttcg gtacagtgta gcctactgtc aggttttgga aacggagaaa
     5341 cgacccgtct tcgatagccc acgacatggg tacagctact gcattgccga atgagaacgg
     5401 cgaccagtat tccttggcat tggcgccttc gttcatctga gccagtgttt cgaggtcgta
     5461 aacaatttcg ccggtttcgt tattcaaata ggtgtaacgg ttgttctgtc gcataaaagc
     5521 aagcatattg ggatttgtgg tcctgtattg ctgtgccgaa gctattttgt tggcattgta
     5581 cacctggttg ccatatacaa agttaaaaag catggtgaaa tcgaaatcat acaccgtacc
     5641 gttcagaccg aaaccgccga ggaatttcgg agcggtgcgt cctattactg tgcggtcgaa
     5701 atcgttcacc acaccgtcgg gttgaccatt tggcccggat aaatctttga atttggcagt
     5761 tccgggacgt gttccgatac gacctccaag cattccgatt gaaggaacac cctggatgag
     5821 ggtgtattta tttgaagccg ggtcaaatgt aaaatctgtg gttttgtaat atccgtcgtt
     5881 tacccaaccg taaattaatc ctaccggttc acctaccttc acctggtaat cgtaatatcc
     5941 tttcaggtcg gtgcctgccc agcctgatgc atattcctgc aaagttattc catcggccag
     6001 tttatctaca tttgatttat tgaaaccgat attgaaattt ccgtaaagtg agaaattttt
     6061 acgttgtgca aaaacggagt tcaaactcag ttctataccc ttgttggtgg tttgtccgat
     6121 gtttctgaac gtagtgcggt agccgggagc cacgattgga atctcgagca gcaggtcttt
     6181 gttggtattc tgatataatt cgaaagttcc cgagagtttt tcgtcgaaga agccgaaatc
     6241 cagaccgata ttctgggtaa cggatgtttc ccagcgcagg tctttgttgg gcagctgggt
     6301 gttggatgta gcccagtagt tattttgaat atcgcccagt ccgtaagttt tcgttgattg
     6361 aatactgtac attttctgga attgtccgcc gcctatttta ttgtttccgg cttcaccgta
     6421 gctgtagcgt agtttcaggt tcgaaatcca gtctttcgtg tttttcataa acggttcttc
     6481 gacaatgcgc catgctgccg ctaccgatgg gaagtaaccc cactgattgc cgggagcaaa
     6541 acgcgacgaa ccatcggcac ggaacgtggc agaaagcagg tatctctctt tgtaattgta
     6601 caccgcacgt ccgaagaatg aaagcagatt gttcggaatg gacacggtgc ttcggatatc
     6661 tttggaagct cctccaagtg ccagattggc aaaaatcttt tcgggtgtga ggtcgggact
     6721 gtagccggtg gcataaatgt atgtgttatt ggctgtgttc gatataagtt cctgaccggc
     6781 cagtacatta atactgtgat caccggcaaa agtttgcata taggtaagcg tattggccag
     6841 acggagtttg ttgctgttgg tttttgtcca gtcaaccagc ggtttgccat ctacatagga
     6901 ggcattggtg gtgttctcgc cccagtagtt tttcacttca ccgaaaccgt attcataacc
     6961 gccttcgatc cggtacacca gactttttat aattttccag tccagcccgc ctataaagtt
     7021 gaaagtacgg tcgttgcggc gtttccagta ttgttgtgcc tgttcggtca acgacatgtt
     7081 ggagcgctgc cattgttcgt attcatcttc ggtcatcagg gcggggtcca ctactatcat
     7141 ttccgacagt ccttttaccg ccggtgaggt gacagcctgc gttgtacgga ttttatacaa
     7201 tccgcccgac gagccggagc cgttgacttt ggtgtcgccg aggcgcaagt tgaacgtcgc
     7261 cttaaggttt ttgtatagct cgtgatccaa tttgaagttg gtggtgaaac gggtgtaatc
     7321 gttgcctgtc atcagaccgc catccttgtt gaaagtggaa cttaaggaga attttgtttt
     7381 atccgttccg ccggtcaggc tgatgttttg ttgctgcgaa accacatccg aaccaaacat
     7441 atcctgctgc cagtcgtgag cacgcatgta tttgtacagg tcgatatctt cgaatactcc
     7501 aaacgttttt tcataagatt tgatgccgtc ttcgcctccg agagctccgt actcatagtt
     7561 aagcaatgca tattcaaatg gatcaagcac ttcgagtcgt ttggagagtc gttttgtttg
     7621 cacaaaaccg ttgtatgaaa ctttggtttt gccaccctgt gcctgtttgg tggttactat
     7681 aaccacaccg ttggcaccct gcgaaccata tatggctgta gatgatgcgt ctttcagcac
     7741 gtcgatactc tgaatgtcgg aaggtgaaat gtcgttgatg ttcgatgccg gaaatccatc
     7801 cacgatatac agcggtgagt tgtcgccggt gatggagcct ccgccacgta cacgtaccag
     7861 aatttcagca tcgggcgaac cgtcggtaga ggttacctgc actcccgcca ttcttcccgc
     7921 catagcctga gcagtacctg taacaggtat ctgagcaata gttttaccct gtatagatgt
     7981 gaccgaaccg gtaagatcgc gcctgcgggc ggtaccgtaa ccgattacca ccacttcatc
     8041 cagtactttc tgatcatctt caagaactac atgaatgaca ttgccggtaa tggctacatc
     8101 cttgggagtt ttacccacat atctcacagt cagtgtagtg gctgaaccgg gcaccgaaag
     8161 gttgtattca ccgtcgatat tggtaattac tcctgaattg gtacctttta ctaataccga
     8221 tgctccgatt atcggctcac cgctgacatc ttttactgtt cctgaaatgt ttcgttgctg
     8281 agccagcaaa ccgctcgcca tcaacatcat tccaagaaat agagcaaaac cttttgccct
     8341 tgatttgtgt ttttgcatca tactgatcgt taaattaaga ttattctttt tctttgaatt
     8401 ggttttctgc ccgtcttgtt gtaccgatct ttattgcgat ctttacatta cctgttatat
     8461 gaattctgtg tgctttttgt gtgctttttt aaattgtgca cgtacacaat attgcaaaca
     8521 caaaaataca acatatattt tcatatcaga taacttactt gttaaatgtt gttaagtgat
     8581 tgaatttata tcctccggat gaaggaaatg aacgaaaaca cgtataatta taaaataaat
     8641 tgtatttttg caggttaaga aatgcagatt taattacaac taacctgaca caaatccggg
     8701 agtgttgaaa taaatcggat tcagccggaa agaaatttat ccgttcggaa aatttcaccg
     8761 aaaaggttca aaaaaacaca gcgcagggct taataataaa caggtaaatg caaaaaagaa
     8821 tcagaatcaa ggacattgcc agaatggcag gagtttccgc cggaacggtt gaccgcatta
     8881 ttcacggcag aggaagtgta tccaaaaaga gcaaggaagt ggtggagagt attctccggg
     8941 aggttaacta ccggcccaac atccacatgt caagcatttc gctcaaaaaa acgtacaaca
     9001 ttctcttcgt ggcacccgga ttcgctgaag gggagtactg ggagagcatt tataaaggca
     9061 tacagcgtgc gctcaacgtt tttgaaaata tctctgtcaa ctgcagtata atgacctata
     9121 atcagtacga cgtgtatgcc tgccgcgaag tgttcgaaag cgcactcgag ccggatttcg
     9181 atgctgtaat tatcggaccg atttttcagc atgaaacccg cgaattctgc cggaaactta
     9241 aaatgaaaaa cattccctat gtttttgtag attccaacgt ggaagacact tcacctctgg
     9301 cctatttctc tgccaatcac tacatttcgg gctatctgat gtgccggctc atccattcca
     9361 tcatgcaaaa cggcaaagac atcggcatca tgcaggccat gcgcgtgggc aacgaaagcg
     9421 ccaactccac catcctacgc aaaaacggat tctctgatta tcaaaaagaa aacaggctga
     9481 aaaataaaat ccaccggatt cctttttcgg ctaccgagcc ggaatcgaac gagcaacttt
     9541 ttaatgattt cttcagtgaa aacgataaca tcggcggcat agtggtgctc aattcacgcg
     9601 gatatgtcat tgccaactat ttgcacaaga agaaaattaa agacatgaaa gtggtgtgtg
     9661 tggatttgac tgatgcaaac aagaaagccc tgaaaaaagg acacatcgat ttcctcattg
     9721 gtcaaaaacc cgaacgacag ggttaccttg ccatgaaatt cctcatcgaa caccttattt
     9781 acaaaattcc ggtaaaagta gccaatatcc tgccgctcga tatactgacc cgcgaaacca
     9841 ttgatctata cagcgaattc aacgacctgg tatatctgga tcagtatgag aagtgagttt
     9901 ggagttctga gtttcgagtt tcgagtttcg agttcggagt tcggagttct gtcccgatag
     9961 ctatcgggaa agttgagggt ttcggtttgt ttactcttta ctccatactc catactccat
    10021 actccatact ccatactcca tactttttac tcttttttct aatttctaat tttaaaatcg
    10081 tatctttgca ctcaattttg aaaaaccatt tgtaaggtac aaaaaaacag atatgataaa
    10141 cattacattc cccgatggtt cggtgcgtcc gtttg
//
LOCUS       DAORKP010000081        10041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_843_length_10041_cov_10.740336, whole genome shotgun sequence.
ACCESSION   DAORKP010000081 DAORKP010000000
VERSION     DAORKP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_10041_cov_10.740336"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2321)
                     /locus_tag="PLN75_05835"
     CDS             complement(249..2321)
                     /locus_tag="PLN75_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRG03670.1"
                     /translation="MRFLLKILFAVVLLLVAGKLEAQDAIKSAEEIIADIYEQISEES
                     ETEIDFTNFFDDLMALSENPINLNSTNKEELDKLQFLSDTQVDNILYYLYKSAPMQTI
                     YELQLIEGLDMTDIRRMLPFVSLGESQARKQLLNLNEMLRYGKNELYFRLDKGLETKE
                     GYRFVPEEENAADENESRKYVGDPYYNHLKYRFRYKDRVQFGVTAEKDAGEQFWGEHH
                     KGYDFFSAHLELRNVGKIKTLVLGDFRANFGQGLVMRTDFSMGKSSYVMQVTPKSNGL
                     KKFSSTSETDFLRGAGATFRFGKVDVTAFYSNRMIDGDTLNGQFSGINESGLHRTLSD
                     LQKKNTVNQQIAGANIVYTHSWFQVGTTAIFNHFSQSLQPRDAIYNRFYFRGQNQLAA
                     SVNYRLRWQKLNIFGETALTDQRAMATINGVNFSPLSRVSLVALHRYFSKEYDALFAN
                     TFSETSRVNNESGMYLGTEIRPVKYWKVSAYVDSYSFSWNKFGVDAPSTGEDYLLQVD
                     YFPKRNVNMYWRFRHETKAHNFSDTLSILPVVLQQPRWQARYQLNYSFGHFDFKNQLD
                     VNGFDDGKNKPTYGFSVFQDVSYDFQKIPLGVNARFHVFDAQSFENRFYTYEKDVLYA
                     FSVPMNYGMGTRYYLNLRYDAGKKLSLWLKLAQTVYADDRTTVSSGNEEITGNRKTDM
                     RFLMRWKF"
     gene            complement(2332..3150)
                     /locus_tag="PLN75_05840"
     CDS             complement(2332..3150)
                     /locus_tag="PLN75_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03671.1"
                     /translation="MKRICLIFFLTACLLPVYAQISPVIPSSVSVAGASSAVMNDWSA
                     FHNPAALAHIDRTEIAAQFENRFMMKELSTKSVQAGFNTGPVNVGLSFSYQGYSVYNE
                     MIAGLGLARNFGDKFSMGVQFDYYTAYFSASDESRYRGAVLAQFGVASTILPKLTVGF
                     NTFNPFQTNIKTEFSTRRIPSVFSIGTNYAFADNLIWLTQIDKEASSNFRFASGFEYT
                     MIRELTVKLGAYGTDYLIPCLGFGIHLGGFHFHLNGELHPLLGLNTVANLKYRF"
     gene            3357..3980
                     /locus_tag="PLN75_05845"
     CDS             3357..3980
                     /locus_tag="PLN75_05845"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRG03672.1"
                     /translation="MKKIFRFAILLTLILTSSGSYGQINVDMTVPGTRPASYAMMEMV
                     VEQGDTIYLDNLRVLYVYPPLVFKSGRQEKFYWRTVRDVKKALPYARLATIEIMNLNR
                     ELYYMPNDAARKKHISDFQSRIFRQYEKPLRNLTINQGKMLIKLIDREHDMNTFDIIK
                     AYKGNMPAFFWNTIARFFGSDLKSEYDASDKDRIVERVVTLVDAGQL"
     gene            4017..4616
                     /locus_tag="PLN75_05850"
     CDS             4017..4616
                     /locus_tag="PLN75_05850"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRG03673.1"
                     /translation="MKTILSKLFSHRKERIKQQKKQIRHEMSLLKKQLSDDQKNNAAE
                     IVFSKIERTRTFLSAKTILVYWSTSSELPTHEFIKKWSREKTILIPSVHGENMTVKKF
                     IAFDEPAARDSEDSDELSEPKTEPYKGKAELAIIPGIAFDLKKNRLGRGGGYYDSYLS
                     AKNIHVFGVGFDFQLMDKIPVKWGSLKMNKIFSPNQVIE"
     gene            complement(4621..5328)
                     /locus_tag="PLN75_05855"
     CDS             complement(4621..5328)
                     /locus_tag="PLN75_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03674.1"
                     /translation="MPYRRLPNTDQARLRALRTVIKQAGQQNFTQQVVTFKSIRDAEN
                     FLHVFEKQLFLYKQTLESQINANKQYQHILYNARMYISHFIQVFNLAVIRGDIKKEHK
                     LIYQLDPEIHNVPDLTTESALQEWGQKIIDGENERIRNGGSPIYNPTIAKVQVHFEVF
                     KEYKSSQKLYQATTTRNWEELVKQRKNGDAIILDIWNQVEEYFRNETPYNKLKKCEKF
                     GLVYYYRRNEKELTPEN"
     gene            complement(5394..6371)
                     /locus_tag="PLN75_05860"
     CDS             complement(5394..6371)
                     /locus_tag="PLN75_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRG03675.1"
                     /translation="MYTFNEVLSIVNTEIEQINWKRVPTGLYEPIEYILSLGGKRLRP
                     AITLMACNLYSDEIMKALKPSIGLEIFHNFTLLHDDIMDRADMRRGKPTVHKKWNDNT
                     AILSGDVMQIEAYKWIACAPEVLLKKILDIFSKTAAEICEGQQFDMEFEQQSDVTIEQ
                     YIEMIRLKTAVLIAAGCQIGALIGGSDDEDAENLYDFGNNIGIAFQLKDDMLDVYGNE
                     ASFGKKIGGDILCNKKTYLLINALKLAEGREKEELHYWIGKNDSEHQHEKIRAVTEIY
                     NRLGVKEVCQKAMVHYYNLAISSIDKVKVPEERKEELKKLAQKLMSRDD"
     gene            complement(6487..7461)
                     /locus_tag="PLN75_05865"
     CDS             complement(6487..7461)
                     /locus_tag="PLN75_05865"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRG03676.1"
                     /translation="MKKNIAIVAGGNSSEVHVSLRSAAGIKSFIDAEKYNCFIVTIVG
                     QVWQVELSESEKYPIDRNDFSFTVGSKKTVFDFAYITIHGTPGENGILQGYFELLNIP
                     YSTCNVLVSALTFNKFTCNQYLKGFGIRVAESVLLRKASSISDEEVIEKIGLPCFIKP
                     NVGGSSFGVTKVKQKTDIQPAIEKAFSEGEEVLIEQFVSGNEFTCGMYKTKTKTVVFP
                     VTEIVTKNEFFDYGAKYNHESEEITPARISAALTERIQTLTNAIYEILGCKGIIRVDY
                     IITENEEINLLEVNTTPGMTTTSFIPQQVSAAGMDIKEVMTDIIENEM"
     gene            complement(7513..9000)
                     /gene="crtI"
                     /locus_tag="PLN75_05870"
     CDS             complement(7513..9000)
                     /gene="crtI"
                     /locus_tag="PLN75_05870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRG03677.1"
                     /translation="MNKKVIIVGAGLGGLASGLLLSKKGFEVEILEMNSQAGGRLNQV
                     QADGFTFDSGPSFFSMSYIFKEFMDKCGVEMPFKFVELDPLYSVNFRGSDKTFHLYKD
                     IKKLAAQFEGIEPDFEQKMERYLKKSGALFHDTVDLVIKNNFDNKLDYITTLMRVNPV
                     HLPVLFESFYGHVKKYFQSKEARQIVSLVAFFLGRTPFDTMGIYSLLSYTEFRHDGYY
                     NVEGGMYNVVKGIVKELEKQNVRIRYSTEIVDVQSDGDKVKKLTDKNGNTYEADTFLI
                     NADAALFRNQILKRPKFSDKKLAKMEWTMGYLTFYVGIKRKLPQVNHHNYYLGGNYEE
                     YANNVLKNPDTLQKPYYYVNVVSKHNAECAPEGCESLFFVCPVPNMQYKPNWDDREEI
                     VNSILTDFSERIGVDILPDIITKTIYTPKEWKEWMNLYQGSGLGLSHKMSQIGGLRPA
                     NFDEKFKNMFYVGASTVPGAGLPMAIISADLACQRIIKQAKSAEK"
     gene            complement(8997..9728)
                     /locus_tag="PLN75_05875"
     CDS             complement(8997..9728)
                     /locus_tag="PLN75_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03678.1"
                     /translation="MKIRLTLFFLLIAFGYTSAASTEQTVIYKAYTSSNMTLWKKTMD
                     SMHDQAGKTNENELELLNYEYGYIGWCIGNKKTAEARKYIDRGQQRIEKLMQKKFKVS
                     MLHAYKSAFYGFEIGLNKAKAPFWGRKSIDEAKRAVATDANNPFGYIQLGNIEFYMPA
                     VFGGSKKKALEYYLKAEKLMTVGDVKNDWNYLSTLTLIAGAYEELGNYEKAETYYLKI
                     LSISPQFTWVKNNLYPKFLSKKSKK"
     gene            complement(9745..>10041)
                     /locus_tag="PLN75_05880"
     CDS             complement(9745..>10041)
                     /locus_tag="PLN75_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRG03679.1"
                     /translation="DWRWGEAYFANKLLDYDLAANNGNWQWVAGCGCDAAPYFRIFNP
                     AEQQKKFDPEMNYVKKWIPEYGTKHYPDPIVEHTFARNRVLERFKKALEIKQNY"
BASE COUNT         2550 a         2190 c         2204 g         3097 t
ORIGIN      
        1 cataaactta acgaactatt gaaaaataag acaatgaata aaccaatttt tcctaaaaca
       61 atattaaaaa gattgttttt ttatcatgaa acagccgatt tgaaaatcgg ctgaaaacac
      121 aagcagaaga ctttttttac cttgctattc tgtaaatcgg actagaatgt ccgatcccct
      181 gttaccataa cattgcaccc tttgcatgca ctcttacccc tccccctgag ggggaggctg
      241 ggtgggggct aaaacttcca cctcatcaga aacctcatat ccgttttacg attgccggtt
      301 atttcctcgt ttccgctgct tacggtagtg cggtcgtcgg catacacggt ttgagcgagc
      361 ttgagccaca atgaaagttt tttaccggca tcgtagcgca agttcaggta gtacctggtt
      421 cccataccat aattcatcgg taccgagaaa gcgtaaagta cgtctttctc gtaggtataa
      481 aagcggtttt cgaaactttg cgcatcaaaa acatgaaaac gggcattcac accgagcggt
      541 attttctgaa aatcatagct tacatcctga aaaaccgaga atccgtatgt cggtttgttt
      601 ttaccatcat caaatccatt gacatccagc tgatttttga aatcaaaatg tccgaacgaa
      661 taattcagct gatagcgtgc ctgccagcgg ggttgctgaa gcactacggg caggatgctg
      721 agcgtgtcgg agaagttatg cgcctttgtt tcgtgtctga atcgccagta catgtttacg
      781 tttcgttttg gaaaataatc cacctgaagc aggtaatctt cgccggttga aggggcgtca
      841 accccaaatt tattccagga aaacgaataa ctgtccacat aagccgatac tttccagtat
      901 ttcacgggtc tgatttcggt tcccagatac attccgcttt cattgttcac ccgagatgtt
      961 tcggagaacg tattggcaaa gagcgcatcg tattctttgg agaaataacg gtgtaatgct
     1021 accaggctaa cccgcgacag ggggctgaag ttcactccat tgatagtggc cattgcccgt
     1081 tgatccgtca gggcggtttc gccgaaaata ttgagttttt gccaccgcag gcggtaattc
     1141 acgcttgcag ccagctggtt ctgtccacgg aaatagaaac ggttgtaaat tgcatcccgc
     1201 ggttgaaggc tctgactgaa gtggttgaat atagccgttg tgcccacctg aaaccagcta
     1261 tgtgtataaa cgatgtttgc accggcaatt tgctggttca cggtgttctt tttttgtaaa
     1321 tcgctcagcg tcctgtgcaa tccgctttca ttgatacccg aaaactgacc gttgagcgta
     1381 tcgccgtcga tcatccggtt ggagtagaaa gcagttacat ccacttttcc gaatcggaaa
     1441 gtggcaccgg cgcctctcag aaaatcggtt tcggaagtgg aactgaactt ttttagtccg
     1501 ttgcttttag gcgttacctg catcacgtac gacgattttc ccatggagaa atccgtacgc
     1561 atcaccagtc cctgcccgaa attagccctg aaatcgccca aaaccagtgt ttttattttg
     1621 ccgacatttc tcagttcgag gtgagcggag aaaaaatcgt atcctttgtg gtgttcgccc
     1681 cagaattgtt cgcccgcatc tttctctgcc gtaacgccaa actgaaccct gtctttgtac
     1741 cggaaacggt atttcaggtg attgtagtag gggtcgccaa catattttcg cgattcgttt
     1801 tcatccgctg cgttttcttc ttcgggcaca aagcggtatc cttctttcgt ttcaagcccc
     1861 ttatcgagcc tgaaatagag ttcgttttta ccgtatctca gcatttcgtt cagattgagc
     1921 agttgttttc tggcctgcga ttcgcccagc gacacaaaag ggagcatccg ccggatgtcg
     1981 gtcatatcaa gcccttcaat gagctgcaac tcgtagattg tttgcatcgg agcgcttttg
     2041 tagagataat acaggatgtt gtcaacctgc gtatccgaca aaaattgaag tttatccagt
     2101 tcttccttgt tggtggagtt gaggttgatc ggattttcgg acaatgccat caaatcgtcg
     2161 aagaaattag tgaaatcaat ttctgtttcc gattcttctg aaatctgttc gtaaatatcg
     2221 gcaatgattt cttcggcaga tttaatcgca tcctgtgctt caagttttcc tgcaacgagc
     2281 agtaaaacta ccgcaaacaa tattttgagt aaaaatctca tgttgaggta attagaaccg
     2341 gtatttcaga ttagccactg tattcagtcc cagcagcggg tgcagttcgc cgttcaggtg
     2401 gaaatgaaaa ccgcccagat gtataccgaa acccagacag ggaatcaggt aatcggttcc
     2461 gtaagctccg agttttacgg ttagttcccg gatcatggtg tattcaaaac ccgatgcaaa
     2521 gcggaagttg ctgcttgctt ccttatctat ttgtgtgagc cagatcaggt tgtcggcgaa
     2581 tgcatagttg gttccgatac tgaaaaccga tggaattcga cgtgtgctga attcggtttt
     2641 tatatttgtc tgaaacggat tgaaagtgtt aaatccgacc gtgagtttcg gtagaatggt
     2701 ggaagctact ccaaactgag ccagcactgc accacgataa cggctttcgt ccgatgcaga
     2761 gaaataggct gtatagtagt cgaactgaac acccatcgaa aacttatcgc cgaagttgcg
     2821 ggctaatccc aggcctgcaa tcatctcatt gtaaacagaa tatccctgat acgagaaact
     2881 cagccctacg ttgaccggac cggtgttaaa gcctgcctgc acacttttgg tgctcaactc
     2941 tttcatcatg aaacgattct caaactgagc ggcaatttca gtgcgatcta tatgggcaag
     3001 tgcagcagga ttatgaaaag cactccagtc attcatcacc gctgatgatg cccctgcaac
     3061 ggataccgaa gaaggtatta caggagatat ttgtgcataa acgggtaata aacaggctgt
     3121 tagaaagaag attaaacaga tacgtttcat agagagaatt ctaaatgttt gtaaaaaagc
     3181 aatacttaaa tttcaatcct ggctaagaag aactaatatg ttttccaaaa gtaataaata
     3241 aattataatt tttcaccgca ataaaaaata aaagttggaa taattattgt tgtatatata
     3301 tggttgtctt gtggtttgtc taaggtttta ctatctttga cgctgattat ttcaaaatga
     3361 aaaagatatt tcgctttgca atccttctga ctttaattct cacttcttca gggagttatg
     3421 gtcagattaa tgtcgatatg accgttcccg gtacacgtcc ggcttcgtat gccatgatgg
     3481 aaatggtggt ggagcagggc gataccattt atctcgataa tttacgcgtt ctgtatgttt
     3541 atcctccgct ggtattcaaa agcggcaggc aggaaaagtt ttactggcgc accgtgaggg
     3601 atgttaagaa agcattgccg tatgcccggc tcgctaccat tgaaattatg aatctgaacc
     3661 gcgaactgta ttatatgccc aacgatgctg cccgcaagaa gcacatcagc gactttcagt
     3721 cacgcatatt ccggcaatac gaaaaaccac tccggaatct gactatcaat cagggcaaaa
     3781 tgcttatcaa actgattgac agagagcacg acatgaatac gttcgacatc ataaaagcgt
     3841 acaaaggcaa tatgccggct tttttctgga atacaattgc gcggtttttc ggctccgatt
     3901 tgaagagcga atacgatgcc tccgacaagg atcgtattgt agagcgggta gttacgctcg
     3961 tggatgccgg ccagctgtag atttttgagt attaacctta ccccatattt tgcacgatga
     4021 agactatttt atcgaagttg ttttcgcatc gtaaagaaag gatcaagcaa cagaaaaaac
     4081 agatccgcca tgaaatgagt ttactgaaaa agcagctgag cgatgatcag aaaaacaacg
     4141 ctgccgaaat tgtgttctca aagattgaac gaacaagaac atttttaagt gccaaaacaa
     4201 ttctggtgta ctggtcaaca tcatccgagc tgcctaccca tgagtttatc aagaagtgga
     4261 gccgggagaa aaccatcctg ataccctcgg tacacgggga aaacatgacc gtgaaaaaat
     4321 tcatcgcttt cgatgagccc gctgcccggg acagtgaaga ttcagatgaa ctttcggaac
     4381 ccaaaaccga gccatacaag gggaaggcag aactggcaat tattcccgga atagctttcg
     4441 acctgaaaaa aaacaggctg gggcgtggtg gaggctatta cgactcttac ttgtcggcca
     4501 aaaatataca tgtattcgga gttggttttg attttcagct gatggacaaa attcccgtga
     4561 agtgggggag tctgaaaatg aataagatat tcagtccgaa tcaggtaatc gaatgaattt
     4621 tcagttttcc ggagtcagtt ctttctcgtt tcggcggtaa taatacacca gtccgaattt
     4681 ctcgcatttt ttcagcttgt tatagggagt ttcgtttctg aaatactctt ccacctgatt
     4741 ccagatgtcc agaataatcg catcaccgtt tttacgttgt ttcacgagtt cctcccaatt
     4801 gcgggtggta gtggcctgat acagcttttg actggacttg tactccttga aaacttcaaa
     4861 atgaacctgc actttggcaa ttgtcggatt gtagatgggc gaccctccgt tgcgtattcg
     4921 ttcgttctca ccatcaatga ttttctgtcc ccattcctgt aatgccgact cagtagtcag
     4981 atcaggaaca ttgtggattt ccggatcaag ttgatagatg agtttatgtt cttttttgat
     5041 atcgcctcgg attacagcca gattgaaaac ctgaataaaa tgagaaatat acattcgggc
     5101 gttgtagagt atgtgctgat actgtttgtt ggcgtttatc tggctttcga gggtttgttt
     5161 gtacagaaaa agttgttttt cgaatacgtg caaaaaattc tcggcatcgc gtattgactt
     5221 gaaagtgact acctgctgag tgaaattttg ctgccccgcc tgtttaataa ctgtacgtaa
     5281 ggctctgagt ctggcctggt cggtatttgg taagcggcgg tagggcattt ttaatcgcaa
     5341 aaattgagat gagatagtta ataaatattg tattttttac aaaagtacga attttaatcg
     5401 tctcttgaca tcagtttttg tgctaatttt tttaattctt ctttccgttc ttcgggcact
     5461 tttactttgt caatgctcga aatggccaga ttatagtaat ggaccatggc tttttggcag
     5521 acttccttca ccccgagccg gttgtaaatc tcagttactg ctctgatttt ttcatgctga
     5581 tgttcggagt cgtttttccc gatccagtag tgaagttctt ctttttctct tccttcagcc
     5641 agtttcaggg cgttgataag caagtaggtt tttttgttac agagtatgtc tcctcctatt
     5701 ttttttccga aactcgcttc gttgccgtac acatcgagca tatcatcctt gagttgaaaa
     5761 gcaataccta tattattacc gaaatcgtaa agattttcgg catcttcatc gtccgatccg
     5821 cctattaatg ctccaatctg gcatccggct gcaatcagta ccgctgtttt cagtcgtatc
     5881 atttcgatgt attgttcgat ggtaacatcg ctctgttgtt caaattccat gtcgaattgc
     5941 tgcccttcgc agatttcggc ggctgttttg gagaatatat cgaggatttt tttcaggaga
     6001 acttcgggtg cacatgctat ccacttgtaa gcctcaatct gcatcacatc gcccgatagt
     6061 atcgccgtat tgtcgttcca ttttttatga acggtcggtt taccgcgacg catgtcagcg
     6121 cggtccatga tgtcgtcgtg cagcagggtg aaattatgaa atatttccag accgattgaa
     6181 ggtttcaggg ctttcattat ctcatccgaa tacaaattgc aggccatcag cgtaatggca
     6241 ggcctaagcc gctttccacc cagcgagagg atatattcaa tcggctcgta aagaccggtc
     6301 ggcacccgtt tccagttgat ttgttcaatt tctgtgttga caatgcttaa aacctcgttg
     6361 aatgtgtaca tagttggtgt attggttgaa aagttaaata gttgatttgt ttgtcactaa
     6421 tcagtattct aaaaagtccc tttcggggga aacaacgttt ggtgtaagcc aattcggatc
     6481 cttatattac atctcatttt caataatatc ggtcatcacc tctttgatat ccattccggc
     6541 ggcgctcact tgctgcggga taaaactcgt ggtagtcatg ccgggtgttg tgttcacttc
     6601 cagcaggttg atttcttcgt tttcggtaat gatgtaatcg acgcgaatta tgcctttgca
     6661 acccagaatt tcgtagatag cattggtcag agtttgtatc cgctctgtga gtgctgccga
     6721 aatgcgggcc ggtgtaattt cctccgattc atggttgtat tttgcgccgt aatcaaaaaa
     6781 ctcatttttg gtcacaattt ctgttacagg aaaaaccact gttttggttt tggttttgta
     6841 catgccgcag gtaaattcat taccactcac aaattgctct atgagcactt cctcgccttc
     6901 ggaaaaggct ttttcaattg cgggttgaat gtcagttttt tgcttcactt tcgttacccc
     6961 gaagctgctt ccgcccacgt tgggtttgat gaagcagggt aaaccgattt tctcaattac
     7021 ttcttcgtcg gatatgctgc tcgccttgcg cagcagaaca gattctgcca cgcggatgcc
     7081 aaatcctttg aggtactggt tgcaggtgaa tttattgaaa gtaagtgccg ataccaggac
     7141 attgcaggta gagtagggta tattgagcaa ctcgaaatat ccctgcagga tgccgttttc
     7201 gcccggagtg ccgtgaatgg tgatgtaagc aaaatcgaat accgtttttt tgctcccgac
     7261 cgtgaaactg aaatcattcc tgtcgatggg gtatttctcc gattccgaca attccacctg
     7321 ccacacttgc cctacaatgg tcacgatgaa gcagttgtac ttttcggcat caatgaacga
     7381 tttgattccg gccgcactcc ggagcgaaac gtgtacttcg gatgagtttc ctccggcaac
     7441 gatggcgata ttttttttca ttgttgtagt ttcctttttt tctgaatgat gaaacaaaat
     7501 tactacaaaa aattattttt cggcagattt tgcctgtttt attattcgtt ggcatgccag
     7561 gtcggcgctg ataatcgcca tcggaagtcc ggctccgggc acggtggatg cacctacgta
     7621 gaacatgttt ttgaactttt cgtcgaagtt ggcaggacgc agaccaccta tctggctcat
     7681 tttgtgcgac aatccgagtc cgctgccctg atacaaattc atccactctt tccattcttt
     7741 gggggtgtaa atggtttttg tgataatatc cggaagtata tccacgccaa tacgttcgga
     7801 gaaatcagtt aaaatgctgt tcactatttc ctcgcggtca tcccagttgg gtttgtattg
     7861 catattgggt acagggcata caaaaaacaa gctttcgcat ccctcagggg cacattcggc
     7921 gttgtgtttc gataccacat tcacgtaata gtagggcttt tggagagtgt cggggttttt
     7981 cagcacgttg ttggcgtact cctcgtagtt gccgcccagg tagtaattgt ggtgatttac
     8041 ctgcggcaat tttcgtttta tcccaacgta aaaagtgagg tatcccatag tccactccat
     8101 cttggccagt tttttgtctg aaaatttcgg gcgtttcagt atttgatttc tgaataatgc
     8161 agcatcggca tttatcagaa aagtatctgc ttcgtaggta tttccatttt tgtcagtcag
     8221 cttttttacc ttgtctccat ccgactgcac atccactatt tcggttgagt accggattct
     8281 cacgttttgt ttttcaagtt ctttcactat ccctttcacc acgttgtaca tgccgccttc
     8341 cacgttgtag tacccgtcgt gccggaattc ggtgtaggag agcaggctgt aaattcccat
     8401 ggtgtcaaaa ggcgttctgc ccagaaaaaa tgccaccagc gatactattt gccgggcttc
     8461 tttggactgg aaatatttct tcacatggcc gtaaaaagac tcaaaaagta ctggaagatg
     8521 aaccggattt acacgcatca gcgtggtaat gtaatccagc ttattgtcaa agttgttttt
     8581 aatcaccaaa tcaactgtat cgtgaaagag tgcaccggat tttttcaggt atcgctccat
     8641 tttttgttca aagtcgggtt caataccttc aaactgtgcg gctagttttt taatgtcctt
     8701 atacagatga aatgttttat cgcttcctct gaaattgacg gaatacaacg gatcgagttc
     8761 cacaaatttg aacggcattt ctaccccgca tttatccata aattccttga aaatatagga
     8821 catactgaaa aaactgggtc cggaatcaaa tgtgaatcca tctgcctgca cctgattaag
     8881 tctgcctccg gcctgggaat tcatttccag tatttctact tcaaatcctt ttttggaaag
     8941 gagcaaaccg ctcgccagtc cgcccaaacc ggcacctaca ataattactt ttttgttcat
     9001 ttttttgatt ttttagaaag aaatttagga tacaaattat tttttaccca ggtaaactgc
     9061 ggtgatatac ttaatatttt gaggtaatac gtttctgctt tttcataatt gccgagttct
     9121 tcgtaagcgc cggctatgag tgtaagcgtg ctcaggtagt tccagtcgtt tttcacatcg
     9181 cctacggtca tgagtttttc tgctttcagg tagtactcga gcgctttctt tttggagccg
     9241 ccgaaaactg ccggcatata aaattcaata ttaccgagct gaatgtagcc aaaaggatta
     9301 ttggcatcgg tggctactgc ccgtttggct tcgtcgatgc tttttcttcc ccagaacggt
     9361 gcttttgctt tattcagacc tatttcaaag ccgtaaaagg ccgatttata agcatgcagc
     9421 atgcttacct tgaatttttt ctgcatcagt ttttctattc gctgctgccc tctatctatg
     9481 tattttcggg cttcagcggt ttttttgttg ccaatacacc agccgatata accgtattcg
     9541 tagttgagca gttccagctc attttcattc gttttgccag cctgatcgtg catggagtcc
     9601 atcgtttttt tccacaaagt catgttgctg cttgtgtaag ctttgtaaat cacagtttgc
     9661 tcggtggagg cagcactggt gtacccaaac gcaattagta aaaaaaagag agtcaaacgt
     9721 attttcattt gcttatggtt tttttcaata gttttgtttg atttcaagtg cttttttgaa
     9781 acgttcgagc acccggtttc gggcaaaagt gtgttccaca atcggatcgg gataatgttt
     9841 ggtgccgtat tccggaatcc attttttcac atagttcatt tcggggtcaa atttcttctg
     9901 ttgttcggcc ggattgaaaa tgcggaaata tggtgcagca tcgcatccgc agcccgctac
     9961 ccactgccag ttgccgttgt tggctgccag gtcgtagtcc agcagtttgt tggcaaaata
    10021 agcttcaccc cagcgccagt c
//
LOCUS       DAORKP010000082        10015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_851_length_10015_cov_7.820683, whole genome shotgun sequence.
ACCESSION   DAORKP010000082 DAORKP010000000
VERSION     DAORKP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_851_length_10015_cov_7.820683"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1250)
                     /locus_tag="PLN75_05885"
     CDS             complement(93..1250)
                     /locus_tag="PLN75_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRG03680.1"
                     /translation="MIKENPYRYKTGTSRTETPLHKTFIILLEIIRLLLAAVFIFSGF
                     VKAIDPLGSTYKFEDYLNAFGGFFGQLTFMAFPAAIALSTLELAIGLCFLLKIKLKTT
                     SLAALLFMLFMLPLTLYVALANPVTDCGCFGDALIISNWATFYKNIVITFLIVILLIF
                     NGKLRRIFLPAIEWILLLLFIASGVALSVYSYSHLPMIDFRPYKVGVNIPAAMQVPPG
                     KPTDKYETTFIYSKDGVSREFTLENYPKNDSTWTFVEQKSTLIEKGYEPPIHNFTIVD
                     ANQIDITDEVIHHKGKVHLLIMYDVSKASEKGVAKTEELYRQAKADNELFYALTASPD
                     EEIEKFAKQHGVTYPFCKTDPITLKTIIRANPGLMVIENGTIKEKRNWRDF"
     gene            complement(1271..1813)
                     /locus_tag="PLN75_05890"
     CDS             complement(1271..1813)
                     /locus_tag="PLN75_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRG03681.1"
                     /translation="MTSTDRQYDHVRAICREIFEKKLKDYGASWRILRQTSVTDQIFI
                     KANRIRSIETKGVNKVGEDIRGELIAIVNYGVIGLIQLELGETTCDDLKPENALELYE
                     KYILRAKQLMEAKNHDYDEAWRLMRIESYTDLILMKINRTKQIEDHQGKTLISEGIDA
                     NYFDMINYAVFGLIKIEFGE"
     gene            complement(1979..3406)
                     /locus_tag="PLN75_05895"
     CDS             complement(1979..3406)
                     /locus_tag="PLN75_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRG03682.1"
                     /translation="MPSLKELFKKATGTNADTEVRLTVSGSNRKYYRLQNKDISLIGV
                     VGISAEENHAFIEMSKHFKAKALPVPEFIIKSDDNLKYLQEDLGDTVLFDYLRKGRLT
                     RVFSSDEKELLHKTMHMLAKIQIKGAEGFDFSICYPQPEFNKRSVLWDLNYFKYCFLK
                     ATGLEFQENRLEDDFDRFADKLLQAPIDCFMYRDFQSRNVMIKEGEPYFIDYQGGRKG
                     PVFYDVASFLWQAKAQYGAELRGELLDTYIKSLGEHRDVNETEFREMLDHFVLFRLLQ
                     VLGAYGFRGYFEKKPHFLQSIPYALENLREVMQHCDFSAFPYLMNVLHEMAALKQFRE
                     VNLDKPLVVTVYSFSYKKGMPNDDSGNGGGFVFDCRAVNNPGKYERFQSLNGLDTPVI
                     QFLEEDGEIIRLLDNAYNMVDTTVKRYMERKFTNLMVSFGCTGGQHRSVYSAQKMAEH
                     ISEKFGIEVNLVHREQNLEQHFERK"
     gene            3888..5846
                     /locus_tag="PLN75_05900"
     CDS             3888..5846
                     /locus_tag="PLN75_05900"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRG03683.1"
                     /translation="MYLRIKINIPDTSKISYGVLQMDIDDAFVAYINGNEIARLGIGT
                     KGDYPAFNQLGANHEATMYAGGSPENFLITKATLRSCLKPGQNVLAIQVHNSSYTSSD
                     MSSNAFLSFAIRDASVIFRPTPFWFVEPDLSGITPDFSSSNLPVIMINTNGAAIIDEP
                     KIPADMKIIFHDDNSRNYLSDPANVYDGKIGIEIRGASSAGFPQKPYGLETRDMTGAN
                     LNVSLLGMPADNDWVLLSNWNDKVYMRNTLAFELFRKMGHYAPRMRHCEVVMNNEYQG
                     IYLFGEKIKQGKPSRVNIAKLTMLDDSGDDVTGGYIFKTDYDDGYGTYWTSSYSPLNR
                     PRGVVRFVYHVPKATDLIDAQKEYIKNHVNTVESVLYGTNFKDPVNGYRKYLDVSSFV
                     DYFIIGELSRNVDAYKKSRFFYKDKDSKDIKIYSGPVWDYDWAWKNMNDCYFLSNTNG
                     TGWAYKINECNVSPVPPSWEVRMLQDPYFANAINDRYFSLRKTILSQSFINNYIDSVA
                     NLLSEAQIRHEKKWKTLGKNVGAPEIDAQPTTFAGDITKFKNWITTRLAWLDANMVGE
                     STAVTELNAYANLQVYPNPVQDELNVKADVNIKRIILLNSLGQYITDVHPANSSHLIN
                     TSNLPRGMYIINVTLSDGHTLTRKIIKE"
     gene            6145..7881
                     /gene="lysS"
                     /locus_tag="PLN75_05905"
     CDS             6145..7881
                     /gene="lysS"
                     /locus_tag="PLN75_05905"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRG03684.1"
                     /translation="MSIELSEQELFRRQSLTELRNLGIDPYPAAEYPTNAFSTEIKAA
                     FNDEEEPKREVCIAGRIMSRRIMGKASFAELQDSKGRIQVYISRDDINTEAQPEMYNT
                     VFKKLLDIGDFIGIKGFVFRTQMGEISIHAQELTVLSKSLKPLPIVKYKDGVAYDAFE
                     DPEQRYRQRYVDLVVNEGIKDIFLKRTRVYQSMRDFFNNEGYIEVETPVLQPIPGGAA
                     ARPFITHHNALDIPLYLRIANELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTA
                     MEIYVAYKDYNWMMDFTERMVEKVCLDVNGTTKVKIGDREIDFKTPYKRISMIDSIRE
                     HTGIDINGMNEEQLFEVCKKLNVEVDDTMGKGKLIDEIFGAKCEGNYIQPTFITDYPR
                     EMSPLCKRHRTNPELTERFELMVNGKELANAYSELNDPIDQLERFQEQLKLSEKGDDE
                     AMFIDLDFVRSLEYGMPPTSGMGIGMDRLVMLMTGQEAIQEVLFFPQMKPEKAAQKDT
                     TDKFTALGIPEDWAPVVQKAGILKVKDLAGLNANKFHQDICGINKKNKMDLKNPSRED
                     VQSWISAAENLA"
     gene            8019..9092
                     /locus_tag="PLN75_05910"
     CDS             8019..9092
                     /locus_tag="PLN75_05910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRG03685.1"
                     /translation="MIAESIEWGNSNISISEVLAETPIRDSSDKARIAIMGGGSWATA
                     LAKIALSNSKYINWYMRRQEQIDEFIRLGKNPSYLTGVKFDTDRITFTSDINKVVRTS
                     DILIFATPSPFLKQHLKKLKRKIDNKIVVSAIKGIVPEENMLISDYFIEKYKVPAENV
                     LVIGGPCHAEEVALERLSYLTIACKSREKARYVADKLVNPYIHTRISDDVYGLEYTSV
                     MKNIYAIASGVCHGLKYGDNFQAVLISNALQEMNRFCTATNPQHRDISESAFLGDLLV
                     TAYSKFSRNRLFGSMIGKGYSVKTAQMEMEMIAEGYYATKTIHELNEKYQVKIPIVEA
                     IYNILYEKRSPVIEIRELTGKMQ"
     gene            9214..>10015
                     /locus_tag="PLN75_05915"
     CDS             9214..>10015
                     /locus_tag="PLN75_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRG03686.1"
                     /translation="MEHIKLTIDKAYGFVQADKVAAYKADAEAANLALQNGTGKGNDF
                     LGWLNLPAEIDEAHLADVENTAKILRDNCEVVVVIGIGGSYLGAKAVIDALSNSFDWL
                     QTKRTSPVVVYAGQNIGEDYLYELQELLKTKRFGIISISKSGTTTEPALAFRLLKTQL
                     EEQQGKEEAKKLIVCITDKAKGALRVLANREGLKTFILEDNIGGRYSVLSPVGLLPIA
                     VAGMDIRQLVQGAVTMQQVCGTGTAFEENPAAQYAAVRNELYRTGKKTE"
BASE COUNT         2923 a         2267 c         2216 g         2609 t
ORIGIN      
        1 ctccggggaa cttaaaagat tttgttgttg ttattgttat tttttttaca aattatgttt
       61 ttaagcctcc atttcggagt ggtttggaga ggctaaaaat ctctccagtt gcgtttttct
      121 tttatcgttc cgttttctat caccatcaat cccggattgg cgcgaatgat tgttttcagg
      181 gttatcggat ctgttttgca gaaaggataa gttacaccgt gctgttttgc gaatttttcg
      241 atttcttcat cgggtgaagc cgtaagagca taaaacagtt cgttgtcggc ttttgcctgc
      301 cggtaaagtt cttcggtttt agccactcct ttttcggatg ctttgctgac gtcatacatg
      361 atgagcaggt ggacttttcc tttgtggtga attacttcgt cggtaatatc gatttggttc
      421 gcatctacga tggtaaaatt atgaatcgga ggttcgtaac ctttttcaat cagcgtggat
      481 ttctgctcca caaaagtcca tgtactgtca tttttaggat aattttccag tgtaaattcc
      541 cggctcactc cgtctttgct gtagataaac gtggtttcgt acttatcggt tggttttccg
      601 ggtggcacct gcatggctgc cgggatattt acacccactt tgtacggacg gaaatcaatc
      661 atgggcagat gactgtagct gtatactgat aaagcaacgc ctgaagcgat aaacagcagc
      721 aataaaatcc actcgatggc aggcagaaat atccggcgga gttttccgtt gaaaatgagc
      781 aaaataacta tgagaaaagt gattacaata tttttataga aagttgccca attggatata
      841 atcagtgcat cgccaaagca accgcaatct gtcaccggat tcgcgagggc tacgtagagg
      901 gttagcggga gcataaacag cataaacagg agggcagcta aggatgttgt tttcagtttt
      961 attttgagca aaaagcacag tccgattgcc aattcgaggg ttgaaagagc tatagctgcg
     1021 ggaaaagcca tgaaggtgag ttgaccgaaa aagccgccaa aggcattcag gtagtcttca
     1081 aatttatagg tggagccgag cgggtcaatc gcttttacaa atccggaaaa gatgaaaaca
     1141 gcagccagca aaaggcggat tatttcaagc aggataatga aagttttatg caaaggcgtt
     1201 tctgtacggg aagttccggt tttgtatctg tatggatttt ctttaatcat tttcaaaaag
     1261 ttaggtacct ttattctcca aattcaattt ttatgagacc gaaaacggca taatttatca
     1321 tatcaaagta gttggcgtca atgccttctg aaataagagt cttaccctga tgatcctcta
     1381 tttgcttggt gcggtttatt ttcatcagaa tcaaatcggt ataggattca atgcgcatca
     1441 gcctccatgc ttcgtcgtaa tcgtggtttt tggcttccat aagctgtttg gccctgagga
     1501 tgtacttttc gtaaagctcc agtgcatttt caggtttcag atcatcgcag gttgtctcgc
     1561 ccagttccag ctgaatgaga ccgatgactc cgtaattgac aatagctatc agctcaccgc
     1621 ggatatcttc gcccactttg ttcactcctt tggtttcgat agagcgtatc cggttggctt
     1681 tgataaaaat ctggtcggtg accgaagtct gacgcaaaat acgccaggag gcaccgtagt
     1741 ctttcagttt tttttcaaat atttccctgc aaattgccct tacgtggtcg tattgccggt
     1801 cggtactggt cataagtggt tgatttgatt ttggtttgca aaattaacaa tttttcggga
     1861 acatcacaaa aatgtagctg ataattaaca ccggacaggt agtcaaaaaa gtttaaataa
     1921 ataaaaaagc acatttcatt taagtgtatt cacccgaaaa gcggttttta atttcttttt
     1981 atttcctttc gaagtgttgt tcgaggtttt gttcgcggtg aaccaaattg acttctattc
     2041 caaatttttc gctgatatgc tcggccattt tctgggctga atataccgac cggtgctgcc
     2101 cgccggtaca tccgaaagat accatcagat tggtgaattt tcgttccatg tatcttttta
     2161 cggtggtatc taccatgttg taggcattat cgagcaggcg gattatttcg ccgtcttctt
     2221 ccagaaattg tatgaccgga gtatcgagtc cgttgagcga ctggaaacgt tcgtattttc
     2281 cgggattgtt taccgcgcgg caatcgaata caaatccgcc tccgttgccc gagtcgtcgt
     2341 taggcattcc ttttttgtag gagaagctat acacggtcac caccagcggt ttatccagat
     2401 tcacttcccg gaactgcttg agggctgcca tttcatgcaa tacgttcatc aggtagggga
     2461 atgccgaaaa atcgcagtgc tgcattactt cgcgcagatt ttcgagtgca tacggaatgc
     2521 tttgcaggaa atgcggtttt ttctcaaaat agcccctgaa gccataagcg ccaagcacct
     2581 gcagcaacct gaagagcaca aaatgatcca gcatttcgcg gaattccgtt tcgtttacat
     2641 ccctgtgctc tccgagcgat tttatgtacg tatcgagcag ttcgccccga agttcggctc
     2701 cgtactgtgc cttggcctgc cagagaaacg aagccacatc gtagaaaacg gggccttttc
     2761 ggccaccctg ataatcgata aagtaaggtt caccctcttt tatcatcacg ttacggctct
     2821 gaaaatcgcg gtacataaag caatcgatgg gcgcctgcag taatttatcg gcaaagcggt
     2881 caaaatcatc ttcgagccgg ttttcctgaa attccagccc ggtagctttc agaaaacagt
     2941 atttgaagta attcaaatcc cagagcaccg atcgcttatt gaactccggc tgcggatagc
     3001 aaatggaaaa atcaaagcct tcagctcctt ttatttgtat ttttgcgagc atgtgcatgg
     3061 ttttgtgcag caattctttt tcgtccgagc tgaaaacccg cgtaagtctc ccttttcgca
     3121 gataatcaaa aagcactgta tcgcccaggt cctcctgaag gtacttgaga ttgtcgtccg
     3181 atttgatgat aaactcaggt acgggcaacg ctttggcttt gaaatgtttg gacatttcaa
     3241 tgaatgcgtg gttttcttcg gccgatatcc ctaccacccc gatgagcgag atgtctttat
     3301 tctgcaggcg gtaatacttc ctgttggagc ctgatacagt gagccttact tcagtatcgg
     3361 cattggtacc ggtggctttt ttgaaaagtt cttttaatga gggcattgaa tcaatttttt
     3421 ttttcatttt tagtaagttt tgcaaattta caaataaaaa ggataccatt gaggtatgaa
     3481 aaatgaattt ttcacaaaac tacaactcaa acgtgttata tgtttggtta gatttgtcag
     3541 cttttcataa aatgagtata tttgcacctg aattgtggtc accacaattt taacctgtct
     3601 atcactattt tttctatgaa aaaacacgtt ggtatattga tattttttca attaactatt
     3661 ttttttccgc tataactcag acaattcatc actgggaaac tgcggttttc aaagatgaca
     3721 gctggaaata tttcgttggg aaatccgagc cagatgctca gtggcgtcaa ctgaatttcg
     3781 acgattcatc ctggaattca ggccagggcg gattcggata tggcgacaac gatgataata
     3841 ccattattga acaggatgca cttcccacat atcagtatcc tccatccgtg taccttcgca
     3901 tcaaaatcaa catccccgac accagcaaga tttcatacgg agtactccaa atggatattg
     3961 acgatgcgtt tgtagcctat atcaacggca atgaaattgc ccggttgggt atcgggacca
     4021 aaggtgacta tccggcattc aatcaacttg gggcaaatca tgaggccacc atgtatgccg
     4081 gtggatcgcc cgagaatttt ctgataacca aagccaccct tcgcagctgc ctgaaacccg
     4141 gccagaatgt attagccatt caggtacaca acagctctta cacatcatcc gatatgtcat
     4201 ccaatgcttt tctttcgttt gccattcggg atgcttccgt catattcaga cccacaccct
     4261 tctggtttgt tgagcctgat ttatcaggaa ttacacccga tttttcctca tccaatcttc
     4321 cggtaattat gatcaatacc aacggtgctg caatcattga cgaacccaaa attccggccg
     4381 atatgaaaat catttttcac gacgacaact cccggaacta tctttctgac ccggcaaatg
     4441 tgtatgatgg taaaatcgga atcgaaatca ggggtgcttc ttctgccggc tttcctcaaa
     4501 agccctacgg actggaaaca cgcgacatga caggagccaa tctgaacgtt tcgctgctcg
     4561 gaatgccggc agacaatgac tgggtgctgc tttccaactg gaatgacaag gtgtatatgc
     4621 gaaataccct tgcttttgaa ttattcagaa aaatgggaca ttatgcaccc aggatgagac
     4681 attgtgaagt ggtaatgaac aatgaatatc agggtattta cctttttggt gaaaagataa
     4741 agcagggcaa accatcccgg gtaaacattg ccaaactcac catgcttgac gactcgggcg
     4801 atgatgtgac aggtggatac attttcaaaa cagactacga cgacggatac ggaacctact
     4861 ggacaagcag ttactctccg ttgaatcgtc cacggggagt ggtgagattt gtatatcacg
     4921 tcccgaaagc taccgacctg attgatgccc agaaagagta catcaaaaat catgtaaaca
     4981 ccgtagaatc ggtgctatac ggcaccaatt tcaaagatcc cgtgaatgga tacagaaaat
     5041 atctcgacgt cagttcattt gttgactatt ttattattgg cgaattgtcg aggaatgtag
     5101 atgcatacaa gaaaagccgc tttttctaca aagataaaga cagtaaggat attaagattt
     5161 attcaggtcc ggtatgggat tacgactggg cctggaaaaa tatgaatgat tgttattttc
     5221 tgagtaatac aaacggtacc ggatgggcat acaaaataaa cgaatgtaac gtgtcgcccg
     5281 tacctccttc gtgggaagta agaatgcttc aggatcctta ttttgccaat gctatcaacg
     5341 acaggtattt cagtcttcga aaaaccattc tgagccaatc tttcatcaat aattacattg
     5401 actcggtagc caatctgctt tccgaagccc agatacgtca cgagaaaaaa tggaaaacgc
     5461 tgggtaaaaa cgtcggagca cctgagattg acgcacagcc taccactttt gccggtgata
     5521 ttacgaagtt caaaaactgg attaccaccc ggcttgcctg gctggatgcc aatatggtgg
     5581 gcgaatcaac agctgtaacc gaactgaatg cctatgccaa cctgcaggtt tatccaaatc
     5641 ctgttcagga tgaattaaat gtgaaagctg atgtaaatat taaacgaata attttactga
     5701 actcactcgg gcaatacatt accgatgtgc atcccgccaa cagcagtcac ctgataaata
     5761 catcaaattt gcctcgtgga atgtatatca tcaacgtaac gctcagcgac ggtcatacac
     5821 tcacccgaaa aataatcaaa gaatagctct gaaaaagctt aaaaaaggaa atttgccgca
     5881 agtttttgtt aagtggaaga taaatgatca cacaacaaac acaagacatc agttgatttt
     5941 aatctgaatc aaatatagtt ttcactcttt tttttgtaac tttgcagtcc gtttttttgg
     6001 atttattgtt gcacagttag ttggttatac aggtaaacgc ctgatataaa aaggtttgaa
     6061 tagttcaatt ccattgagca aaatctgacc ggttaactta tcaattcaat aaaccatcaa
     6121 cttaacaaac agaagagact aacaatgagc atagaattaa gcgaacaaga actattcaga
     6181 cgtcagagtc tgaccgaatt gcgcaacctg ggcattgacc cctaccccgc tgccgaatat
     6241 ccaaccaacg ctttctccac cgaaataaaa gccgctttca acgatgagga agaacccaaa
     6301 cgcgaggttt gcatagccgg aagaatcatg agccgccgta ttatgggtaa agcatccttc
     6361 gcagaactgc aggacagcaa aggacgaata caggtttaca taagccgcga cgacataaat
     6421 accgaagccc agccggagat gtacaacacc gttttcaaaa aactgcttga catcggtgat
     6481 tttatcggca tcaaaggatt tgtattccgc acccagatgg gcgaaataag cattcatgca
     6541 caagaactta ccgtgctgag caaatcgctc aaaccactcc ccatcgtgaa atacaaggac
     6601 ggcgtggctt atgatgcatt cgaagatccg gagcaacgct accgtcagcg ctatgtggac
     6661 ctggtagtga acgaaggcat taaggatata ttcctcaaac gtacccgcgt atatcagtcc
     6721 atgcgcgatt ttttcaacaa cgaaggctac atcgaggtag aaactcccgt actgcagccc
     6781 attccgggcg gagcagccgc caggcccttc atcacgcatc acaatgcact cgatataccg
     6841 ctttatctgc gcattgccaa cgaactttac ctgaaaaggc ttattgtagg cggatttgaa
     6901 ggagtgtatg aattctccaa aaacttccgc aacgaaggga tggaccgcac gcacaatccg
     6961 gagtttacgg ctatggaaat ttacgttgct tacaaggatt acaactggat gatggacttt
     7021 accgagcgga tggtggaaaa agtgtgtctg gatgtaaacg gcactaccaa agtgaaaata
     7081 ggtgatcgcg aaatcgactt caaaactccc tacaagcgca tatcgatgat tgacagcatc
     7141 cgggaacaca ccggcattga catcaacggg atgaacgaag aacaactttt cgaagtctgc
     7201 aaaaaactga acgtagaagt ggacgacacc atgggcaaag gtaagctcat cgacgaaatt
     7261 ttcggtgcca aatgcgaagg caactacata cagcccacct ttatcaccga ttatccgcgc
     7321 gaaatgagcc cactctgcaa gcgtcaccgc accaatccgg agctcactga gcgattcgaa
     7381 ctgatggtga acggcaagga actggccaat gcctactcgg aactgaacga cccgatcgac
     7441 cagctcgaac gtttccagga acaactgaaa ctctccgaaa aaggcgacga cgaagccatg
     7501 tttatcgacc tggattttgt acgctcgctg gaatacggca tgccccccac ttcgggaatg
     7561 ggtatcggta tggaccggct cgtaatgctt atgaccggcc aggaagccat tcaggaagta
     7621 ctcttcttcc cccagatgaa accggaaaaa gcagcgcaga aagacacgac agataaattc
     7681 acagcactcg gcatacccga agactgggca ccggtagtgc agaaagccgg aattctgaaa
     7741 gtgaaagacc ttgcggggct caatgcgaac aaatttcatc aggatatatg cggcattaac
     7801 aaaaagaaca aaatggacct gaaaaatcca tccagagaag atgtacaaag ctggataagt
     7861 gcggctgaaa acctggcctg agaatttatt acacaaaaca tcttcccgaa aaacaaaccg
     7921 actgatttac acgttatctg attactgaag tcattttcag gaatccagat aaccgaccga
     7981 attcaatagt aattttcgta aaatcataat tcaaccaaat gatagcagaa agcattgaat
     8041 ggggaaattc aaacatttca atttcggagg tattagccga aacgcccatc agagatagtt
     8101 ctgacaaggc acggatagct ataatgggag gcggcagctg ggctaccgcc ctggccaaaa
     8161 tagcgctttc taattcaaaa tacatcaact ggtacatgcg ccgacaggag caaatcgatg
     8221 aatttatccg gttgggcaaa aatccatcgt acctcacggg agtgaaattc gataccgacc
     8281 gcatcacatt caccagcgat ataaataaag tggtgcgcac gtcggacata ctgatatttg
     8341 ccactccctc cccttttctg aaacaacacc tgaaaaaact gaagcgaaaa attgacaata
     8401 agattgtggt ttcggccatc aagggcattg ttcccgaaga aaacatgctt atttcggatt
     8461 attttatcga aaaatacaag gttccggcag agaatgtgct ggtgataggc ggtccgtgtc
     8521 atgccgaaga agtggcactc gagcggctct catacctcac catagcctgc aaatcgcgcg
     8581 agaaagcacg ttatgtggcc gataagcttg taaatcccta catccatacc cgcataagcg
     8641 acgatgtata tggtctggaa tacacttcgg tgatgaaaaa tatctacgcc atagcatcgg
     8701 gcgtatgcca cgggctgaag tacggcgata actttcaggc ggtgctcata tccaatgcac
     8761 tgcaggaaat gaaccggttc tgtacggcca ccaatccgca gcaccgcgac atcagcgagt
     8821 cggcttttct gggcgattta ctggttaccg cctattcgaa attcagccgc aaccggctct
     8881 tcggatcaat gatagggaaa ggttactcgg taaaaacagc acaaatggaa atggagatga
     8941 tagccgaagg ttattacgcc acaaaaacca tacacgaact caacgagaaa tatcaggtga
     9001 aaattcccat tgtggaagcc atctacaata tactctacga aaaaagatca cccgtgattg
     9061 aaataaggga acttaccggg aaaatgcagt agcgaaagcc tcctaaaatc ccgcgaaggc
     9121 agacatactg ctcttatatt taacagataa ttattatacc tgaactccaa aatctcccct
     9181 tcgggagggt tagggggcaa taaaattatt aatatggaac atatcaaatt aacaatcgac
     9241 aaagcttacg gatttgtaca ggccgataaa gtggctgcct acaaagctga tgccgaggct
     9301 gccaacctgg cactccagaa tggaaccggc aaaggcaacg actttctggg ctggctcaac
     9361 ctgcccgccg aaattgacga agcacacctg gctgatgtgg aaaacacagc taaaattctc
     9421 cgcgacaact gcgaagtggt ggtggtaatc ggtatcggcg gcagctacct gggcgccaaa
     9481 gccgtaatcg acgcactctc caacagcttc gactggttgc agaccaaacg cacatcgccc
     9541 gtagtagtgt atgccgggca gaatatcggc gaagattacc tctacgaact gcaggaactg
     9601 ctgaaaacaa aacgattcgg catcatctct atctccaagt ctggaacaac caccgaaccc
     9661 gcgctggcat tccgcctgct gaaaacacag ctggaagaac aacagggcaa ggaagaggct
     9721 aaaaaactga tcgtgtgcat caccgacaag gcaaaaggtg cactccgtgt gcttgccaac
     9781 cgcgaaggac tgaaaacttt catccttgag gacaacattg gcggacgcta ctcggtactc
     9841 tcgcccgtgg gattactccc cattgctgtt gccggaatgg atatccgcca gctcgttcag
     9901 ggagcagtaa ccatgcagca ggtttgcggc accggaacgg ctttcgaaga aaatcctgct
     9961 gctcagtatg cagccgtgcg caacgaactc taccgtacgg gcaaaaaaac agaaa
//
LOCUS       DAORKP010000083         9941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_869_length_9941_cov_9.846854, whole genome shotgun sequence.
ACCESSION   DAORKP010000083 DAORKP010000000
VERSION     DAORKP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_9941_cov_9.846854"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(619..1737)
                     /gene="dprA"
                     /locus_tag="PLN75_05920"
     CDS             complement(619..1737)
                     /gene="dprA"
                     /locus_tag="PLN75_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRG03687.1"
                     /translation="MNDLLKYQIGITLIKGIGPNLAKNLIAYLGGVEAVFKEKKQNLA
                     KIPGIGDVLSAEITSQDVMSRAEMEVEFILKNKIETYFFTQKSYPYRLKECPDSPVML
                     YYKGTANLNEGKFIGIVGTRKATEAGKDICKNIIADLSEKAGNITVVSGLAYGVDICA
                     HKSSLDVHTATIGVLGHGLDRIYPSVHRSTAVRMIQHGGLLTEYMSETNPDKQNFVMR
                     NRIIAGMCDALIVIESGRRGGSLITAQLANDYNRDVFAVPGRIGDEYSAGCNMLIKQN
                     KASLIESAEDVQRFMNWEDTSTPGSKMAVQTQLFENLSDDEQTIIASLRRFPDGININ
                     ELTVQLNKPYSRLSSQLLEMEFKGLVKCLPGGMYRVVK"
     gene            complement(1822..2205)
                     /locus_tag="PLN75_05925"
     CDS             complement(1822..2205)
                     /locus_tag="PLN75_05925"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRG03688.1"
                     /translation="MPNIFLRTYWKSILLCFIIFLLSSVTFKSIPEAARFQYSDKLIH
                     ALMYVALGVVAFFEYTKDTTFKVKYKHWLKFVFISLVLFGGVIEILQGTLLKPRTAEL
                     ADWIADIIGLSAGLGAGRLLFGRKE"
     gene            2303..3121
                     /locus_tag="PLN75_05930"
     CDS             2303..3121
                     /locus_tag="PLN75_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRG03689.1"
                     /translation="MKKVIPILLVVLTLTSCSTVLLTGRRQLNLVSDAELNQMSFAAY
                     KELKDSVPLSADKVNSAMVNRVGDNLKAAVEKILVANGAESEIQNFEWEYNMFKVNQV
                     NAFAMPGGKIAVYDGILPKTMNEAGMAVVVGHEIAHVVAKHSAERLSQQVMAQYGGAI
                     LGAAVGGKSAAVQQGIGALYGIGVQAAVLLPYSRKQEYEADMLGLYIMAVAGYDPDEA
                     LTFWQRMTADNTQSSSDFMSTHPSDDKRIAQLQVVIPEAKKFYKTGLVPVLQKK"
     gene            complement(3175..3825)
                     /gene="upp"
                     /locus_tag="PLN75_05935"
     CDS             complement(3175..3825)
                     /gene="upp"
                     /locus_tag="PLN75_05935"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRG03690.1"
                     /translation="MKVVNLSETNSVLNQFLKEIRSVDIQKDSMRFRRNMERMGEIMA
                     YEISKTMNYKPDKIKTPLGTLESMKLDEKIVISTILRAGLPFHQGFLSYFDTAENAFV
                     SAYRKYKDALKFDIFIEYIASPKLDGKTLIITDPMLATGSSMELAHGALLTKGKPKKV
                     IIATIIASQPAIDFMKNHFSDDVTLWTAAVDPGLNEHSYIIPGLGDAGDLAYGEKL"
     gene            complement(4048..5709)
                     /locus_tag="PLN75_05940"
     CDS             complement(4048..5709)
                     /locus_tag="PLN75_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG03691.1"
                     /translation="MIEKLLQQTDFESFEDFKTNYKLIVPDQFNFAYDIVDAWAAREP
                     DKKALLWTNDQGECREFTFGEIKTHTDQTASYFQTLGLKKGDIVMAILKRRYEFWFTI
                     IALHKIGVTIIPATHLLTKKDLVYRNNAADITAIIAVGEEPILNAINDAMPESPTIRH
                     LISIGPLVPQGWEDFHKGKQNAPEFVPNPRVNKNDDNMIISFTSGTTGNPKMVALDYL
                     YPLGHIITARYWHNLHEKSLHLTIADTGWLKAVWGKLYGQWIVGACVFVYDHEKFAPA
                     DILRKIEKYKVTSLCAPPTIFRFLIREDLTKYNLSSLEYCTIAGEALNPEVYNQFYKL
                     TGIKLREGYGQSETTLSVLTTPWVEPKPGSMGLPSPQYDVDILTPDGRSAEVGEEGQI
                     IIRTDKSYPVGLFKGYYRNKQLTDECFHNNIYYTGDVVWKDEDGYFWFVGRADDVIKS
                     SGYRIGPFEVESALMTHPAVVECAITGVPDEIRGQVVKATIILAQNYRDKAGEELIKE
                     IQNHVKQVTAPYKYPRIIEFVDELPKTISGKIRRTEIRAKDNAAK"
     gene            complement(5725..6273)
                     /locus_tag="PLN75_05945"
     CDS             complement(5725..6273)
                     /locus_tag="PLN75_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRG03692.1"
                     /translation="MNDQIRQIAERLRGLREALEMNTEEFATRAGVNQTEYERYESGN
                     FDIPMSLLFGVAQTFGIDTTELLAGETARASVFFVTRKGTGISVERRKAYKYQALGAG
                     FTGNKSEVFEVTVEPGDKAISLNSHTGQEFNYVLQGTMQLHIAGNDLVLYEGDSIYFD
                     ASKPHGMKALNGEKVVFLAVIL"
     gene            complement(6280..6900)
                     /locus_tag="PLN75_05950"
     CDS             complement(6280..6900)
                     /locus_tag="PLN75_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRG03693.1"
                     /translation="MNTFFKIITYIFQPLLIPTYGIILLMQLSLFQLFPTSYRLFAIA
                     GTVLFTCILPSLPILFMMKNRQISDIFISKREERTMPYLFSLLAYIFWVLFLWRTLQF
                     PMEFVLLAIGSIVSVVLMVFINLKWKISAHTAGMGGLIGGIFGVAFVAAINPVWIITA
                     AIIIAGLVAISRILLKAHTLSQVIGGFFLGFACVFIPVIVYNFLFK"
     gene            complement(7063..8514)
                     /gene="rpoN"
                     /locus_tag="PLN75_05955"
     CDS             complement(7063..8514)
                     /gene="rpoN"
                     /locus_tag="PLN75_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRG03694.1"
                     /translation="MLRQQLQQKLQQKLSPAQIQVIKMLEIPTLELEERIRQEIEENP
                     VLEEGSENAETNDDQDTNQADEADSNDDFDLDEYMSDDDIPDYKLIASNVSRDDKHED
                     IPFSAGFTFHEHLIDQVGLLHLNDQQRKLVEYIIGNIDEEGYLRRDAGSMADDIVFQA
                     GVETNDDEMKQLIKIVQQLDPAGVGAANLQECLQLQLERKEPSEPVKNARRIISDYFE
                     EFSKKHYDKIIRSMSMTEESLKDALAEITRLNPKPGNAWGGNVLEKSFSTIVPDFIIE
                     NDNGVITVQLNNSNVPELRINSTYNEMFEDYTNNKSNQTREMKDAVMFVKQKIDAARW
                     FINAIRQRQQTLLTTMTAIVDFQREFFLEGDETYLKPMVLKDIADITGYDISTISRVS
                     NSKYVQTEFGIFPVKFFFSESMTNDSGEEISTREIKKIMQECIDEEDKRNPLNDDRLV
                     EVLKEKGYLIARRTAAKYREQLNIPVARLRKEI"
     gene            8809..9261
                     /locus_tag="PLN75_05960"
     CDS             8809..9261
                     /locus_tag="PLN75_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HRG03695.1"
                     /translation="MYSKDELKQLKKDFWEGFGLFCANHPVLGKRKSKFMLYNTKMKG
                     VELKFDANREGAFVILEFNHSSETDRLSKYEQFERYKVLMEEEFPEGLIWDFAYRLET
                     GNEVCRIYSQKTGIDIHRRIQWMDFYRFMAEEMLKLERAFREVKDVID"
     gene            9291..9896
                     /locus_tag="PLN75_05965"
     CDS             9291..9896
                     /locus_tag="PLN75_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRG03696.1"
                     /translation="MNHLLNQIEFIREIDKLKYIFRKTKLINSDRPENDAEHSWHLAM
                     MAIVLAGHANEPIDLEKVMKMVLIHDIVEIDSGDVFAYDTTKSHDNFDEELKAARRIF
                     GILPEEQAEEFINLWIEFEEMKTPESKFARALDRLEPLLQNASNNGGTWREFDVKYDK
                     VIEKKKAIKDGSEELWKFAENLIDESVEKGILSSPLTPKGG"
BASE COUNT         2796 a         2119 c         2146 g         2880 t
ORIGIN      
        1 cactcgaaaa aataccgacc ctgcaaactc caataaactg ggaagaactg ctcccgtaaa
       61 accaaataaa acaaagataa aacggactta gagagtgatg ttactctaag tccgtttttt
      121 atctataacg gcggatttgg gatggttaca aaaaaataca cataaactgt tgttgcgctt
      181 cacttcatca cccggtacat tcctccgggc aggcaacgga gatacaaata agggagtttt
      241 ctaattaaaa tttcatactc ttccaggaca caaacattcc gttttccgtc tgaaaattca
      301 gatcaccacc ataaactacg gcaagtgaat ttggattgtt cgtggaataa atattatctg
      361 acaattttat gcaaataaat aaatccgatt tatatatttg catacattta aagttaaatc
      421 agcaattatt tttcaaattc catcaaacat tctgttctac ctatgacaat ctgactgaat
      481 ttgtgataaa tttcctgagt acattcattc cctgttcttg taaccaccgg aactgttaca
      541 ccttttgaaa aaaatacgat aacatccaaa ctcccgatag ctatcgggac aaactttcga
      601 accggataaa ctgatttctc atttcaccac cctgtacatc cctccgggga ggcattttac
      661 cagtccctta aattccattt cgagaagctg cgatgagagg cggctgtagg gcttattgag
      721 ctgtacggtg agttcgttga tattgattcc gtccggaaag cggcgcaggc tggcgataat
      781 ggtctgctca tcatccgaca gattttcgaa aagctgggtt tgaacagcca ttttggagcc
      841 cggtgtggag gtatcttccc agttcataaa ccgctgcaca tcctcagccg actcgatgag
      901 cgaagctttg ttctgcttga taagcatatt gcagccggcg gaatattcat cgccaatgcg
      961 gcccggaaca gcaaaaacat cgcggttgta atcgttggcc aactgagcgg taatgagcga
     1021 ccctcctctc ctgcccgatt ctatcacaat aagtgcatcg cacatgccgg caatgatgcg
     1081 gttacgcatc acaaaattct gcttatcagg attggtttcg ctcatgtatt cggtaagcaa
     1141 accgccatgc tgaatcatcc tgacggccgt agaacgatgt accgacggat aaatgcggtc
     1201 cagcccatgc cctaatacac ctattgtggc tgtatgcacg tccaaagaag atttgtgggc
     1261 acagatatcc accccgtaag ccagtccgct cacaacggtg atattacctg ctttttccga
     1321 caaatcggca atgatattct tacagatgtc cttaccggct tcggtggctt tgcgggtacc
     1381 tacaatgcct atgaatttgc cttcattcag gttagcggtg cccttgtaat aaagcatcac
     1441 cggcgaatcg gggcactctt tcagacggta aggataggat ttttgagtga aaaaataggt
     1501 ttcgatttta tttttgagaa tgaattccac ttccatttcg gccctactca tcacatcctg
     1561 cgaggtaatt tcggcggaaa gaacatcacc gattccggga attttggcga ggttttgctt
     1621 tttttctttg aagactgctt ccacaccgcc cagataggca atcaaatttt tggccagatt
     1681 aggtccaatc ccctttatca gcgttattcc aatctggtat ttaagtaaat cattcattgg
     1741 aggttaagta gtaaatttgt ttcgcatgga atgtatttat aacgaatccg gggtacagat
     1801 aatcgggcaa ctgaattatt tttattcttt tctcccaaat aacaacctgc ccgctccgag
     1861 tccggcggat aaaccaataa tatcggctat ccagtcggcc agttcggcag ttctgggttt
     1921 gagaagagta ccctgcagaa tttcaatgac tccaccgaaa agaacaagcg aaataaatac
     1981 aaactttagc cagtgtttat attttacttt gaaagtagta tctttggtat attcgaaaaa
     2041 ggctaccaca cccagggcta catacatcaa agcgtgaata agcttatcgc tgtactgaaa
     2101 ccgggcagct tcggggattg acttaaatgt aactgaagac aacaaaaata taatgaaaca
     2161 caggaggatt gatttccagt aagtacgtaa aaaaatattg ggcataataa ttgtttttgt
     2221 ttcaattata tttatcttta caaagataat ttattttatc aaaaccatat catttttttt
     2281 taactgaatt aacacatcaa tcatgaaaaa ggtaattcca attttattag ttgtacttac
     2341 actgacatcc tgctccaccg tgttgcttac cggtaggagg caattaaatc tggtatctga
     2401 tgcagaatta aaccaaatga gttttgcagc atacaaagaa ctgaaagatt cagttcctct
     2461 ttcggccgac aaggtaaact cggcgatggt aaatcgtgtg ggtgataatc tgaaagcagc
     2521 tgttgaaaaa atactggttg caaacggtgc tgaaagcgaa attcaaaatt tcgaatggga
     2581 atataacatg tttaaggtca accaggtaaa tgcatttgca atgcccggtg gaaaaattgc
     2641 ggtttacgat ggtattctgc ccaaaacgat gaatgaagcc ggaatggccg tagtggtagg
     2701 tcacgaaata gcccacgtag tggcaaaaca cagcgccgaa agactcagtc agcaggtgat
     2761 ggctcagtat ggtggcgcta tactcggtgc agccgtgggc ggcaagtcgg cagctgttca
     2821 gcagggaatt ggtgcgctct acggaatagg agttcaggca gccgtgctgc tcccttactc
     2881 acgcaagcag gagtatgaag cagatatgct cggtctttac atcatggctg tagccggcta
     2941 cgatcccgat gaagcactta cattctggca acgaatgaca gctgataata cacaatcctc
     3001 atcggacttt atgagtaccc acccatcgga tgataaacgt attgcacaat tgcaggttgt
     3061 gattcccgaa gcgaagaaat tttataaaac agggctggtt cctgttttgc aaaaaaaata
     3121 acaggctgta tgttacaaca aaaaagagag aatcgcttct ctcttttttg tttatcacag
     3181 tttctctccg taggcaagat ctccggcatc gcccagtccg ggaatgatgt aggagtgttc
     3241 gttcagcccc ggatcaacag cggcagtcca gagcgtaaca tcgtcggaaa aatgattttt
     3301 catgaaatcg atggccggct ggctggcaat gatagtcgca ataatcactt ttttcggttt
     3361 gcctttggta agcaatgcac cgtgggccaa ctccatcgaa ctgcccgtag ccagcatcgg
     3421 atcggtaatg atgagggttt taccgtccag tttcggagag gctatgtact ctatgaaaat
     3481 atcgaatttc agtgcatctt tgtattttct gtaggccgat acaaaggcat tttcggcagt
     3541 atcaaagtag gagagaaatc cctggtgaaa tggcagtccg gccctgagta tggtcgaaat
     3601 tacaattttt tcgtcgagtt tcatcgattc aagcgtaccg agcggagttt ttatcttatc
     3661 gggcttgtaa ttcatggttt tgcttatttc ataagccatt atttcaccca tccgttccat
     3721 gttccggcga aatcgcatgg aatctttctg aatatccacc gaacggattt ctttgagaaa
     3781 ctgattcaga acagagttcg tttctgataa attgactact ttcatattga atggtattat
     3841 attcttttgt attgattaac agaagagttt atatgatttt ttgaactact cagcccccca
     3901 acccctaaaa aaggggagtt ttggcgcgac aaacaacatt tatgcatttg aatttggcag
     3961 ttctattgta agtattataa tttttgcatc tgcaattgtt ctgaaaatgc taaataaatt
     4021 acttatcgat gtgctgaata gttacgatta tttggcagca ttatcctttg cccttatctc
     4081 tgttctccgg attttcccgc tgattgtttt cggcagttca tctacaaatt cgatgattcg
     4141 cggatattta tacggtgccg tgacctgttt tacatgattc tgaatctctt ttatcaattc
     4201 ttcgcctgct ttgtccctgt aattctgagc cagaatgatg gtggctttca ccacctgccc
     4261 gcgtatctcg tcgggaacgc ccgtgatagc acattctacc actgcggggt gggtcataag
     4321 agcactttcc acttcgaacg gacctattcg gtaaccggaa ctcttaatca catcgtcggc
     4381 acgccccaca aaccagaaat agccgtcttc gtctttccat accacgtcac ctgtgtaata
     4441 aatgttgttg tgaaagcatt catccgtcag ctgtttattt cggtaatacc ctttgaaaag
     4501 cccaacggga taagatttat cagttcggat gataatctgt ccctcctcac ctacttcagc
     4561 ggagcgtccg tcgggtgtca gtatatcaac gtcatactgc gggctgggca gtcccatgga
     4621 accgggtttg ggctccaccc agggcgtggt aagcaccgaa agtgtggttt cgctttgtcc
     4681 gtaaccttca cgcagtttaa ttcccgtaag tttatagaac tgattgtaaa cctccgggtt
     4741 cagagcttcg ccggctatgg tgcagtactc cagcgacgaa agattgtatt tcgtcaggtc
     4801 ttcgcgaatg agaaaacgga atatggtagg tggtgcacac agggaggtta ccttgtattt
     4861 ctcaatttta cgaagtatat ctgccggtgc aaatttttcg tgatcataaa caaatacaca
     4921 cgcccccact atccactgtc cgtaaagttt gccccatacg gccttgagcc agccggtatc
     4981 ggctatggtg aggtgcagac ttttttcgtg gaggttgtgc cagtatctgg ccgtaatgat
     5041 gtgcccgagc ggatacaggt aatccagtgc taccattttc ggattgccgg tggtgcctga
     5101 agtaaaactg atgatcatgt tgtcatcatt tttatttacc cggggattcg gtacaaattc
     5161 aggtgcgttt tgttttcctt tgtgaaaatc ttcccagccc tgcggaacca aaggcccaat
     5221 agaaatcagg tgtctgatgg ttggagattc gggcattgca tcgttgatgg cgttcagtat
     5281 tggctcttcg cctacagcta tgatcgcggt aatgtcggca gcattatttc ggtacacgag
     5341 gtcctttttg gtaagcaaat gcgttgcagg tatgatggtt accccgattt tgtggagggc
     5401 aatgatggta aaccagaatt cgtagcgccg cttaagaata gccatgacga tatcgccttt
     5461 tttaagcccc agtgtctgaa aataagatgc tgtctgatcg gtgtgtgttt ttatttcgcc
     5521 gaaagtaaac tcgcggcatt cgccctgatc gttggtccaa agcaacgctt ttttgtccgg
     5581 ctccctggca gcccatgcat ccactatatc gtaagcaaag ttgaactgat cgggcacaat
     5641 cagcttgtag ttagttttga aatcttcaaa actctcaaaa tcagtttgtt gtaataattt
     5701 ctctatcatg attttttgaa atgattagag tataacagcc agaaatacta ccttttcgcc
     5761 attcaacgct ttcatgccat gaggttttga agcgtcgaaa tagatgctgt cgccttcgta
     5821 aagcaccaga tcattgccgg cgatgtgcag ctgcatagtt ccctgcagga cgtagttaaa
     5881 ctcctgacct gtatgcgaat tgagcgaaat tgccttatca ccgggttcta cagtaacttc
     5941 aaaaacttcc gatttgtttc ctgtaaaacc ggctcccaat gcctgatatt tgtatgcttt
     6001 tctcctctcc acacttatcc cagtaccttt tcgggtaaca aaaaataccg atgcacgggc
     6061 agtttcaccc gccagaagtt cggtagtgtc aattccaaaa gtctgagcca ccccaaagag
     6121 caaactcatg ggaatatcaa agttgccgct ttcgtatcgt tcgtactcgg tttgattcac
     6181 tcctgctctg gtggcaaact cttcggtatt catttcaagc gcttcgcgca aaccccgaag
     6241 ccgttcggca atctgcctga tttgatcatt catatttttt tatttgaata aaaagttata
     6301 aactattact ggaataaaaa cgcatgcgaa acccagaaaa aaccctccaa tgacctgcga
     6361 tagtgtatgg gctttgagta aaatgcgtga aatggcaacc agacccgcaa tgattatggc
     6421 tgctgtaata atccacaccg gattaatggc cgccacaaaa gctactccaa agatgccccc
     6481 gatcaaacct cccattccgg cagtatgggc gcttattttc catttcaggt tgataaaaac
     6541 catcagcaca accgacacga tggaaccgat ggcaagtaaa acaaattcca tgggaaactg
     6601 cagcgtacgc cacaggaaaa gcacccagaa aatataagcc agcagcgaaa acaaataagg
     6661 catggtacgc tcctcccgtt tagaaatgaa aatatcactt atctgccggt ttttcatcat
     6721 aaaaagtata ggcaacgatg gtaatatgca cgtaaaaagc actgtcccgg caatggcaaa
     6781 taagcgataa gaggtgggaa acagctgaaa aagagaaagt tgcatgagaa ggattattcc
     6841 gtaagtggga atgagaagcg gctgaaaaat ataagtgatt attttgaaga atgtattcat
     6901 ttcgtttttt ttatttaccc tttgggtatt taattagtgg caatctggct gtgtaatagc
     6961 tggaaaaatg aaaaaaatga aatttttccg gatattttga ttcaaaagtg cacgacgact
     7021 attgaaaaat tatgaaatca acaggcaaag ccgattaaga aattaaattt ctttacgcag
     7081 ccgggctacc ggaatgttga gttgctccct gtacttggct gcagtgcgcc tggctatgag
     7141 ataccccttc tctttcagca cctctaccag ccggtcgtcg ttgagcgggt ttctcttatc
     7201 ttcttcatca atgcactcct gcatgatttt ttttatttct ctggttgata tttcctcgcc
     7261 cgaatcgttt gtcattgatt cagagaaaaa gaatttgacg ggaaatattc caaattctgt
     7321 ttgcacatat ttgctgttgc ttacacgcga aatggtagaa atatcatatc cggttatatc
     7381 ggctatatcc ttcagtacca ttggtttgag gtaagtttca tcgccttcca gaaagaattc
     7441 gcgctgaaaa tccacaatgg cagtcatggt agtgagcagt gtttgctgcc gctgcctgat
     7501 ggcgttgata aaccaacggg cggcatcaat tttttgcttc acaaacatca cggcgtcttt
     7561 catttcgcga gtctgattgg atttattgtt cgtgtaatct tcaaacattt cgttgtacgt
     7621 gctgttaatt ctcagttcgg gcacgtttga attgttgagc tgtacggtaa tgactccgtt
     7681 gtcattttca ataataaagt caggtacgat ggtcgaaaaa gatttttcga gcacattacc
     7741 gccccacgca tttccgggtt tggggttgag gcgtgtaatc tctgccagcg catctttcag
     7801 actttcttcc gtcatactca tcgagcgtat gattttgtcg taatgttttt ttgaaaattc
     7861 ctcaaagtaa tcgctgataa ttcttctggc attttttacc ggttcggatg gttccttcct
     7921 ttcgagctgg agctggaggc attcctgcag attggcagct cccacaccgg cgggatcaag
     7981 ttgctgtaca atttttatca actgcttcat ctcatcgtca tttgtttcca cgccggcctg
     8041 aaatactata tcatctgcca tcgacccggc atcgcgacgc aggtaaccct cttcgtcgat
     8101 gtttccgatg atgtattcca ccagttttcg ctgctggtcg ttcagatgca gaagccccac
     8161 ctgatcaatt aggtgttcat gaaaagtaaa tccggcagaa aaaggaatgt cctcatgttt
     8221 atcatcacgt gatacattgc ttgcaatgag cttataatca ggtatatcat cgtccgacat
     8281 atattcatcc aggtcaaaat catcgtttga atccgcttca tcagcctggt ttgtatcctg
     8341 gtcatcgttc gtttcggcat tttcggagcc ttcttccaac accggatttt cctctatttc
     8401 ctggcgaatg cgctcctcca gttcaagagt aggaatttcc agcatcttga ttacctgaat
     8461 ctgtgccggt gaaagttttt gctgtagttt ctgctgtaat tgttgtctta acataaaaaa
     8521 aagtaaacaa gtaaacgagt tgacaagtta acaagaattt aagattgctc cctgtcaatc
     8581 cgattagatc gtacaaagat acattttttc atttaaaact gaagtttgaa cgttgaaaat
     8641 aaaacaaatc tcatgtattg cggttcacga aagaaccgat ttttattgaa aatttctcaa
     8701 agctttcagc ttctcatctt tgagaaaaag aatttattga gtagttttgc aagttgagaa
     8761 ataaaactct gcctctgact caaactgtgg caaagcgacc attcaagcat gtacagcaaa
     8821 gacgaactta aacaacttaa gaaagatttt tgggaaggat ttggtctgtt ctgtgccaat
     8881 catccggttt tgggtaagcg aaaaagcaaa tttatgcttt acaataccaa aatgaagggg
     8941 gtggaattga aattcgatgc caacagggaa ggtgcttttg ttattttgga attcaatcat
     9001 tcaagcgaaa ccgataggct ttcgaaatac gagcagtttg agcgttacaa agtgctgatg
     9061 gaagaggaat ttcccgaagg gctgatttgg gattttgctt atcggctcga aacaggaaac
     9121 gaagtttgcc ggatctacag ccagaaaacg ggcatcgata ttcaccgccg tattcaatgg
     9181 atggattttt accggtttat ggccgaagaa atgctaaaac tggaaagggc gttccgggaa
     9241 gtgaaggatg tgattgatta gacatcatct ctcagaaaaa agacagaaaa atgaaccatt
     9301 tgctcaacca aatcgaattt atccgggaaa ttgataaact taaatacatt ttccgaaaaa
     9361 cgaaactaat taacagcgac cggccggaga atgatgccga gcacagctgg cacctggcaa
     9421 tgatggcgat tgttcttgcc gggcacgcca atgagccgat agacctggaa aaagtgatga
     9481 aaatggtgtt gattcatgat attgtggaaa ttgacagcgg cgatgtgttt gcgtatgaca
     9541 cgacaaaaag ccacgataac tttgatgaag aactgaaagc tgcccgtcgt atttttggga
     9601 ttttacctga agaacaagct gaagaattta ttaatttgtg gattgaattt gaagagatga
     9661 aaacgcctga aagtaagttt gcaagagcac tcgaccgcct ggaaccattg cttcaaaacg
     9721 catccaacaa cggcggaacc tggcgtgaat ttgatgtgaa atacgataaa gtgattgaga
     9781 aaaaaaaggc aattaaggac ggatcagagg aactttggaa atttgccgaa aatctgattg
     9841 atgaaagcgt agaaaaaggt atattgagca gccccctaac ccctaaaggg gggtaaaaag
     9901 gagcaatttt caaatgtttt ctaaaattca aaattgtgtt t
//
LOCUS       DAORKP010000084         9891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_884_length_9891_cov_9.208215, whole genome shotgun sequence.
ACCESSION   DAORKP010000084 DAORKP010000000
VERSION     DAORKP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_9891_cov_9.208215"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..2215)
                     /locus_tag="PLN75_05970"
     CDS             complement(179..2215)
                     /locus_tag="PLN75_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03697.1"
                     /translation="MKYPITCILIILLLNSTLYASDTLTEHQDSTIQLNEIVVDAYRI
                     NTRLQKIPGNIAVLTANETSLSDGNNFTPALHSTPGIYMHSGTYGTSRIVIRGVGSRT
                     PYNTNRIKSYLNDIPITTSDGISTPEDIDLLSIQRIEIVKGPASALYGSGMGGNINLY
                     TPSEKEKSAKGSIQYGSFNTMKVGASGIVNSRNFNLFGSLNHLTSDGYRENNHFIRTS
                     AVSSGRWTLPHHSLEYTLLLMNVIAGIPSSIGKTLYNTHPQAAAVQWNLIKGYKKLSK
                     AIGGITLAKKITPNVSNRFTVFTRWIDSYEKRPFNNLDDGTFSIGWREKLSFHSEKWD
                     FAAGAEFVNDTYNWQLDKNNALINKNSENRNQLNIFGIAYFRPSEKWNISAGSALNKV
                     NYSLTDQFAENSDQSGTRNFPFIFSPRLGINYTPTPQIALYSSVGSGFSMPSPEETLL
                     PEGKINQAIQPEQGIQAEVGVRLNLYDSNTQLDLAVYQIDLTNLLVTKRLTEDIFTGI
                     NAGKTRHRGFELMLKQNLLQLQSFPGNLNLTTSYTYSDNRFIDFTDGGISYNDNSLPG
                     IPSHLAQSTFNWNPAKSLHVQTTFHYVGSQYMNDANTEKSDGYFTIQLKTAYRFSLKK
                     IGNIEIYGGVNNLTDARYASMISVNALSVGGNEPRYYYPGLPRHFFAGLRFNFN"
     gene            complement(2235..2573)
                     /locus_tag="PLN75_05975"
     CDS             complement(2235..2573)
                     /locus_tag="PLN75_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRG03698.1"
                     /translation="MKTNDWKDKLGTLLPEDFKPEPAPEATETKIRYENQLLRIELDK
                     RKGKPATLVTEFQGTDDELKELAKVLKNKCAAGGSQRDGEILIQGDFRVKVAEILQNM
                     GFKVKKINFK"
     gene            complement(2664..3401)
                     /locus_tag="PLN75_05980"
     CDS             complement(2664..3401)
                     /locus_tag="PLN75_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03699.1"
                     /translation="MRIRNFILTATLVVSHFIYSQSGVQKETYQAFLNNDETRWRNVT
                     TKFEKSADLNESADLLQLINYYYCYVSVLIDKQMDKQADENIRKAEVYISKVLKSEPN
                     NALGINYKGIFQSYQVSLNKMKAATMGKQILGNLNKAYSLDPNSVQILFDRGNSFYYP
                     PKVLGGNKKEALNYFQKAISLIEKQKNTHQNWMYLQLLFLEAHCNELLGNTDLARKGY
                     EKALKTEPNFKLVRDKYYPDFLKKYGN"
     gene            complement(3432..3989)
                     /locus_tag="PLN75_05985"
     CDS             complement(3432..3989)
                     /locus_tag="PLN75_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRG03700.1"
                     /translation="MRKLKITELNRLTTEEFKHTEKTPLTVILDNVRSLHNVGSVFRT
                     SDAFLVECVYLCGITSTPPHAEIHKTALGAENTVNWKYCEDALGAVRELKTKGYTIFA
                     IEQAEGSTSLEKINPHRDQKYAVVLGNEVKGVQQAVIDECHACLEIPQFGTKHSLNVS
                     VTAGIVIWEFANKLGLSSAGRFNEK"
     gene            complement(4009..5025)
                     /locus_tag="PLN75_05990"
     CDS             complement(4009..5025)
                     /locus_tag="PLN75_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG03701.1"
                     /translation="MSKLFRYILLLPALISVLQACMKEPDIQPNTNKGNLDALWQIID
                     RRYCYLDYKNINWDSIYTEYAVRLDTVTNKYGLFDLLGEMLLNLKDGHVNLYSEFDRS
                     RYWKWYTDYPENFNSSLIYTNRYLGNNYRIAGGIRYRKIAGDRIGYMYYSSFSNSFSD
                     ANIRSVFEVFAGCKGLIIDVRGNGGGLVSNAALLASYFFDEKKLTSYISHKTGNGHSD
                     FSEPTAVYTEPHKKIKWERPVVILTNRMSYSATNEFVCRMKYAPKATIAGDKTGGGGG
                     LPLSSELPNGWMVRFSASPMYDAGMINTEWGIDPDVKVNLSKQDEANGYDTIIEKAVS
                     LILD"
     gene            complement(5018..5866)
                     /locus_tag="PLN75_05995"
     CDS             complement(5018..5866)
                     /locus_tag="PLN75_05995"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HRG03702.1"
                     /translation="MKKSTILVIFLFIFVLIRAQQFTDEKYALTTTATSVGAGFITLL
                     DPYLSPFEYKGFEMRIENKSRRFFNPDNDKLSYSHRMYLELGKGNHPSGKNSMLFFNT
                     NYMYGLNYHMRPVENLMILAGGSWDIDLGGKYLGRNVNNPFSLDFFSDLNATAEIQYE
                     FDLKNRSIRIQYGAVCPVAGFMFVPMQNVSYYELFVLDNTKNAFHFSSVHNKRAWYQY
                     LNLDIPFKSNTLRLSLQHDYQKYSANEVIYRKNAISLSVGAVMHLYTFRGNKDRIPTH
                     FIHSYE"
     gene            6098..6796
                     /locus_tag="PLN75_06000"
     CDS             6098..6796
                     /locus_tag="PLN75_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRG03703.1"
                     /translation="MNRKKELVVLTGAGMSVESGLSTFRGGGGLWDNHKVEDVATPEA
                     WKRNPELVLNFYNLRRKELLAVKPNAGHLALAELEKEYDVKIITQNVDNLHEKAGSTN
                     VLHLHGELMKVRSTGPGEEIFTLSDDNYETHPGDVCPEGFQLRPHIVWFGEAVPAMDQ
                     AIRTVEEADIMLIIGTSMQVYPAAGLIHYVRSGVPVYLIDPNEVGVYGRDISVIKKGA
                     GEGMKDFMNRLKNK"
     gene            6793..7890
                     /locus_tag="PLN75_06005"
     CDS             6793..7890
                     /locus_tag="PLN75_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03704.1"
                     /translation="MKVKSATLKLMLIITLVVVNVSIVWPQSEKLSAGDNSHELTVYV
                     FPTLKPLDWTSPASLYKSTIDLYLKTMFLTDHYLLGHMAVKLQTPLLDKPLLTAQTSC
                     GMEEKRKLLFNEKIGLGILGAALKGRMEPADELMHNLNVYARRDKLAFIKYKLNEKST
                     RRIIDFIKMYSSNMMGRQSQSGFYGGSFWPRYKYEGAGCSAFAIAILELAGIQPPDSC
                     GWKINVNIPMKLIGGKFNQGKKIKSGEIKRTLLWHSGEGEKNVDFVSYNIYDPTYIYD
                     WINNKIQQLNSGYKVVSENGISGLLTDATTVECNEDEPVFLHNDSPDLFVATHFRENK
                     EFPFAEISADSLQRMQIPEILKLDSTAVIRK"
     gene            8066..8257
                     /gene="rpsU"
                     /locus_tag="PLN75_06010"
     CDS             8066..8257
                     /gene="rpsU"
                     /locus_tag="PLN75_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRG03705.1"
                     /translation="MIVVPVKEGENIEKALKKFKRKFEKTGVVKELRRRQCFDKPSII
                     KREQKNHAVYVQQMQRSED"
     gene            8342..9241
                     /locus_tag="PLN75_06015"
     CDS             8342..9241
                     /locus_tag="PLN75_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRG03706.1"
                     /translation="MISLFLQYLQYEKNYSSHTVLSYRTDLFQFCDFLGTDPSEFNPA
                     LVSQSTIQQWILSFMERGVSPRSISRKISSLKSFWRFLMKRGLVDKNPALKLVLPKTN
                     KPLPAFYKQQEMDNVLSENNRTGGFEAVRNRMIIDLFYETGMRVSELVSLTDSSVDFD
                     ARQLRVIGKRNKERIIPLGETICQDLRVYQEIRNRVTDIKCDALFVRKNGMKMYSKAI
                     YNIVHNEMAQFSTLKKQSPHVLRHTFATILLNNGADINAVKDLLGHSSLAATQVYTHV
                     SFSELNKIYKQAHPRAIKKGGQI"
     gene            9238..9537
                     /gene="raiA"
                     /locus_tag="PLN75_06020"
     CDS             9238..9537
                     /gene="raiA"
                     /locus_tag="PLN75_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRG03707.1"
                     /translation="MKFRIQSINFDATEKLEAYINKKAMKLSKVFDEIMTMDVYLKVV
                     KPETATNKEAEIKVTAPNVEFFASKTCDTFEEAVDLGLEAIEKQVIKYKEKLAKK"
     gene            9652..9725
                     /locus_tag="PLN75_06025"
     tRNA            9652..9725
                     /locus_tag="PLN75_06025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9686..9688,aa:Thr,seq:tgt)
BASE COUNT         2834 a         1978 c         2105 g         2974 t
ORIGIN      
        1 gttgacgata tgtcgtatgt atcaaccggt ggcggagcgt tgctcgaagc cattcaggga
       61 ttggtactgc ccggcattgc agctatcaag ggataaaata aaaatctcca caaaaaaaga
      121 atatttcaaa aaaaatgctc tgaagaaagt cgaaggcgaa tgcttttcgg ctttcttttt
      181 agttgaaatt aaatctcaat cctgcaaaaa agtgtctggg caaccccggg tagtaatatc
      241 ttggctcatt tccgccaact gaaagggcat tgaccgaaat catcgaagca taccgggcat
      301 cggtcagatt gttcactcct ccataaattt caatatttcc aatttttttc aacgaaaacc
      361 gataagcagt tttcagttga atggtgaaat atccatccga tttttcggtg ttagcatcat
      421 tcatatactg tgacccgacg taatgaaagg tggtttgaac gtgcagcgat tttgccggat
      481 tccagttgaa agtactctga gcgaggtgcg aaggaattcc cggaagtgaa ttgtcgttgt
      541 atgagattcc gccatcagtg aaatcgatga acctgttatc ggaatatgta taactggttg
      601 tcagattcag atttcccgga aaggattgaa gttgcagcag gttctgtttc agcattaatt
      661 caaagccccg atgacgcgtt ttacctgcat tgatgcctgt aaaaatatct tcagtgagcc
      721 gctttgtcac cagtaaattt gtcaggtcaa tctgataaac cgccagatcc agctgtgtat
      781 tgctgtcata cagattgagt ctcacaccaa cctctgcctg aatgccctgt tcgggttgaa
      841 ttgcctgatt gattttccct tcgggaagga gtgtttcttc gggtgaaggc atggagaatc
      901 cgcttcctac cgatgaataa agtgctattt gcggtgttgg agtgtagttt atacccagac
      961 gtggcgaaaa tataaacggg aaattacgtg tcccgctttg gtcactgttt tctgcaaact
     1021 ggtctgtaag cgagtaattc actttattta atgcgcttcc tgccgaaata ttccattttt
     1081 cagagggtct gaaataggct atcccaaaga tattcagctg gttgcggttt tcggagtttt
     1141 tatttatcaa cgcattattt ttgtcgagtt gccagttgta ggtatcgttt acaaattcgg
     1201 ctcctgctgc aaaatcccat ttttccgagt ggaaagacaa tttttctctc caaccgatac
     1261 tgaacgtgcc gtcatccaga ttgttgaaag gacgtttctc atagctatca atccatcgtg
     1321 taaaaacagt aaaccgattg ctcacattgg gggttatttt tttagccagc gtaattccgc
     1381 caatcgcctt gctgagtttt ttatagcctt tgattaaatt ccattgaaca gctgccgcct
     1441 gcggatgggt gttgtacagc gttttcccga tggaacttgg tattccggcg ataacattca
     1501 tcagcagcag agtgtattcc agcgagtggt gaggcagagt ccatcgtccc gacgaaacgg
     1561 ccgaagtccg gatgaaatga ttgttttccc gataaccatc cgacgtcagg tggtttaagc
     1621 tgccgaacag attgaaattt ctgctgttca caattccact tgcacctact ttcatcgtat
     1681 tgaaactgcc gtactgaatg cttccttttg cacttttctc tttttcggaa ggtgtgtaca
     1741 ggttgatatt gcctcccatg ccggaaccat agagtgcaga tgcaggccct tttactatct
     1801 caattcgttg aatgcttagt aaatcaatat cttcgggagt cgaaattcca tcggaggtcg
     1861 ttatcggaat atcattcagg tatgatttta tccggttggt gttgtagggc gtacggcttc
     1921 ccacaccacg tattacaatc cgactggtac cgtaagtgcc gctgtgcata taaatgccgg
     1981 gtgtggaatg gagtgctggg gtaaaattgt tcccatccga aaggctggtt tcattagctg
     2041 tcagcacagc aatatttccg ggtatttttt gtaatcgggt attgatccgg tatgcatcta
     2101 ctacgatttc attcagctgt atggtggagt cctggtgctc agtgagtgta tcacttgcat
     2161 ataaagtgct gtttaacagc aatataataa gtatgcaggt gattggatat ttcatttatt
     2221 taaaatgact gtttttattt gaaattgatt ttcttcactt tgaatcccat gttttgaagt
     2281 atctcagcta cttttacacg gaaatcgccc tgaataagaa tttcgccatc gcgctgtgaa
     2341 cctcctgcag cacatttgtt tttcagtact ttagccagct ctttcaattc atcgtcagta
     2401 ccctgaaatt cagtaacaag cgttgccggt ttgccctttc tcttgtcgag ttcgatgcgc
     2461 agtagctggt tttcgtatcg gatttttgtt tcagtagctt caggtgcagg ctcaggtttg
     2521 aaatcttccg gcaataatgt gcctaatttg tctttccagt cgttggtttt cataagagct
     2581 aagtgattat atgtctgaaa atcatttttg tatgttgtaa aacccggttt tcactgatac
     2641 ggattgtttg aaagttcatg caatcagttt ccgtattttt tcaaaaaatc agggtaatat
     2701 ttatcgcgta caagtttaaa gtttggctcc gttttaagtg ctttttcgta ccctttccgt
     2761 gccagatccg tattccccag caattcattg cagtgagcct cgagaaacaa caactgaaga
     2821 tacatccagt tttggtgcgt atttttttgt ttttcgatga gtgatattgc tttttggaag
     2881 taattcaatg cttccttttt gttgcctccg agtacttttg gagggtaata aaacgagttt
     2941 ccacggtcaa aaagtatctg tacgctgttc ggatcgaggc tgtatgcctt gttgaggttg
     3001 cccagaattt gctttcccat ggtggcggct ttcattttat tcaaagatac ctgatagctc
     3061 tgaaaaatac ctttgtagtt gattccaagc gcattattcg gttctgattt caataccttg
     3121 ctgatgtaaa cttcagcttt cctgatgttt tcatctgcct gtttgtccat ttgcttgtct
     3181 atgagtaccg atacgtagca gtaataataa ttaattagtt gcagcaggtc agctgactcg
     3241 tttaagtcgg cacttttctc aaattttgta gtcacatttc tccacctggt ttcatcgttg
     3301 ttcagaaatg cctgatatgt ttctttctga acaccacttt gactgtaaat aaagtgcgaa
     3361 accacaagcg ttgcagtcag tatgaagttt ctaattctca ttatttttta ttttttgtta
     3421 cttgtaaaaa attacttctc attgaaccgg cctgccgaac tcaatccgag tttgtttgca
     3481 aattcccaga ttactatccc agctgtaacg cttacgttca aagaatgttt tgttccgaat
     3541 tgcggaattt caaggcatgc gtgacattca tctatcaccg cttgctgcac acctttcact
     3601 tcgttgccca gcaccacggc gtatttctga tccctgtgag gatttatttt ctccagcgaa
     3661 gtgcttcctt cggcctgttc aatggcaaat atagtgtatc cttttgtctt cagttcgcgt
     3721 actgcgccga gagcatcttc acagtacttc cagttcaccg tattttcggc tcccagggct
     3781 gttttgtgta tctcggcatg tggaggagtg gatgtaatgc cacaaagata tacgcattcc
     3841 accagaaatg catcggaagt tctgaaaaca gatcctacgt tgtgcaggct tcgtacattg
     3901 tcgagtatca ctgtgagtgg tgtcttttct gtatgtttga attcctcggt tgtcagccgg
     3961 tttaattcag taatcttcag cttgcgcata ataaagtata aatccttttt aatctaaaat
     4021 gagtgaaact gctttttcta ttatggtgtc gtatccgttg gcttcgtcct gtttgcttaa
     4081 attcactttc acgtccggat caattcccca ttcggtatta atcattccgg catcatacat
     4141 ggggctggcc gaaaaccgta ccatccagcc attcggaagt tcgcttgata aggggagtcc
     4201 gcctcctccg cccgttttgt cgcctgcaat tgtagctttc ggagcgtatt tcatcctgca
     4261 tacgaattcg ttggtggcac tgtaagacat acggttggtg agaattacca ccggacgttc
     4321 ccattttatc tttttgtgtg gttcggtata tactgctgtg ggttccgaaa agtcggaatg
     4381 gccgtttcct gttttatggc tgatatagct tgtgagtttt ttttcatcga aaaagtaaga
     4441 agccagcagt gcagcattcg acaccaggcc tccaccattg ccccttacat caataatcag
     4501 acccttgcac ccggcaaaga cttcaaaaac tgaccggata tttgcatcgc tgaaactgtt
     4561 cgagaaacta ctgtaataca tatatccaat cctgtcgcct gctattttgc ggtatcggat
     4621 gccacccgca atccggtaat tgtttcccaa atagcggttt gtatagataa gactggagtt
     4681 gaaattttcg ggataatcag tgtaccactt ccagtaacgg ctgcggtcaa attccgaata
     4741 caggtttacg tggccatctt tgagattgag cagcatttca cccagcaggt cgaaaagtcc
     4801 gtatttgttt gtaaccgtat ccaggcgaac agcgtattcg gtgtagatgg agtcccagtt
     4861 gatgtttttg taatcgagat aacagtatcg cctgtcaatg atctgccaca gggcatccag
     4921 attcccttta ttggtattcg gttgaatatc aggttctttc atgcaggctt gaagcacact
     4981 gatcaatgca ggcagaagga gaatatatct gaacaattta ctcatacgaa tggatgaaat
     5041 gcgtaggaat tctatcttta ttgcccctga atgtgtataa atgcattacc gctcccacag
     5101 ataaactgat tgcatttttt cggtaaatta cttcattggc agaatatttt tggtaatcat
     5161 gctgcaaact tagtcgcaat gtgttggatt tgaaaggaat atctaaattg agatactgat
     5221 accatgcacg tttgttgtgg acagaactga aatgaaatgc atttttggtg ttatccagca
     5281 caaacaattc gtaataactt acattttgca tcggtacaaa cataaatccc gctaccggac
     5341 atacagcacc gtactgaatg cggattgacc ggtttttcaa atcaaattcg tactgtattt
     5401 cggcagtagc gttcaaatcc gaaaaaaagt cgagcgaaaa cggattattc acattccttc
     5461 ccagatattt acctcccaaa tcaatgtccc agctccctcc ggccagtatc atcagatttt
     5521 ccaccggacg catgtgataa tttaatccgt acatgtaatt tgtattgaaa aacaacatgc
     5581 tgtttttacc actcggatga tttcctttac ccagttcaag atacatgcgg tgagaatagg
     5641 aaagtttgtc gttgtccgga ttgaaaaaac ggcgggattt gttttcgatt ctcatctcaa
     5701 aacctttata ctcaaaaggt gaaagatagg gatccagcag tgtgataaat ccggctccga
     5761 cggaggtggc ggtggtggta agtgcgtatt tctcatcggt aaactgctgt gcccgaatta
     5821 aaacgaatat gaacaggaag atgacaagta tcgttgattt tttcatcaga aagtccgttt
     5881 tgctgttgtg tgcaaaagta agaaataatt ttttaataac ctcgttgctg ttttcggaaa
     5941 aagatgagtg atttttttag aaaatccggt ttagtaggga cgaaaattta atttaacgtt
     6001 gaaccctttt tctgaaaaaa taaacagaga atcctttcgt atggcagaaa aatagtattt
     6061 ttgtgaacat ttcaaaacct gaaaaacgat ttgagtcatg aacagaaaaa aggaattggt
     6121 ggtgcttacc ggagcaggca tgagtgtgga aagtggatta tctactttca gaggcggagg
     6181 aggcctctgg gataaccata aagtagaaga tgtagccact cccgaagcct ggaagcgaaa
     6241 tccggagttg gttctcaact tttacaatct tcgccggaag gaactactgg cagtaaagcc
     6301 caacgccgga catctggcct tggccgaact tgaaaaagaa tatgatgtca agatcatcac
     6361 gcagaatgtg gataatctgc acgaaaaagc cggaagcacc aatgtactgc acctgcacgg
     6421 cgaactgatg aaagtccgct caaccggtcc gggagaagaa atttttacgc tgagcgatga
     6481 taactacgaa actcacccag gcgatgtatg tccggaagga tttcagctgc gaccgcatat
     6541 agtttggttt ggcgaagccg tacctgctat ggatcaggca ataaggactg tggaagaagc
     6601 cgatatcatg ctcataatag gtacttccat gcaggtatat cctgccgccg gactcattca
     6661 ctacgtacgt tccggtgtac ctgtctattt gattgatccc aacgaagtgg gagtttacgg
     6721 acgcgatatt tcagtaataa aaaaaggagc cggcgaaggc atgaaagatt ttatgaaccg
     6781 gctgaaaaac aaatgaaagt aaaaagcgca acacttaagc taatgttgat aattacactc
     6841 gtagttgtca atgtcagtat tgtatggcct caatccgaaa aattgtcagc aggcgataat
     6901 tcgcatgaat taacagtgta cgtttttccc acattaaagc cactcgactg gaccagtccg
     6961 gcaagcttat acaaatcgac catcgatttg tatttgaaaa caatgtttct gactgatcat
     7021 tatttactcg gacacatggc tgtaaaactt caaacaccac tgctggacaa acctctgctt
     7081 acggcgcaga cttcatgcgg gatggaagaa aagcgaaaac tgctgttcaa tgaaaaaatt
     7141 ggtctcggaa tacttggggc ggcactgaaa ggacgcatgg agccggcaga tgaactgatg
     7201 cataatttaa acgtatatgc ccggcgggat aaacttgcct tcattaaata caaactgaat
     7261 gaaaaaagca cccggagaat tattgatttt attaaaatgt attcgtcgaa catgatggga
     7321 agacaatcgc agtcaggatt ctacggtgga tccttttggc caaggtacaa atacgaagga
     7381 gccggttgct cggcttttgc aatagccata ctcgaactcg ccggaataca acctcccgat
     7441 agctgcggat ggaaaataaa tgtgaatata ccgatgaaac tcattggcgg caagtttaat
     7501 cagggtaaaa aaataaaatc gggtgaaata aaacgcacgc tgctatggca tagcggagag
     7561 ggagaaaaaa atgtggactt tgtcagttac aatatctacg atcctactta tatctatgat
     7621 tggataaata ataaaataca acaactcaac tcaggctata aggtagtgtc tgaaaacggt
     7681 atttcgggct tgctgaccga tgctacgact gtagaatgca acgaagatga acctgttttt
     7741 ctccacaacg attctcccga tttgtttgtc gctactcatt tccgggaaaa caaagaattc
     7801 ccatttgccg agatttctgc cgactctttg caacggatgc agattcccga aattcttaaa
     7861 ttggattcaa ctgctgtaat tcggaaataa aacaccttta ggcagataat acaatggcaa
     7921 atacagcagc atgtacatcc tgaatgcagt ccggatttca tatgaaataa aatattgttt
     7981 ttagttttgg aatttgaaaa tgattctgta tctttgcaga ccaaaaaaat aagcaaccca
     8041 aaaacaatta aaataagatt taattatgat tgtagtacct gtaaaagaag gcgaaaacat
     8101 tgaaaaagca cttaaaaaat tcaaacggaa atttgaaaaa accggagttg taaaagaatt
     8161 gcgtcgtcgt cagtgttttg ataagccttc tatcattaaa cgtgaacaaa agaatcacgc
     8221 tgtttacgtg caacaaatgc agagaagcga agattaactg aaattttttt tcgaaaagtt
     8281 ggtatattga taaaattatt ctattttcgt ttgttgttag cacaacggaa attttatcgg
     8341 tatgatcagc ctgtttttac aatatttaca gtatgagaag aattattcat ctcatactgt
     8401 tttgtcatat cgcaccgatt tgtttcagtt ctgcgatttt ctcggaactg atccatccga
     8461 attcaatccc gcccttgttt cacagagcac cattcagcaa tggatattat ctttcatgga
     8521 acgcggtgtt tcaccccggt ccatatcaag aaaaatatcg tctctcaagt cattctggcg
     8581 ttttttgatg aaaagagggc tggtcgacaa aaatccggca ttaaaactcg tattgccaaa
     8641 aacaaataag ccacttcctg cgttttacaa acaacaggag atggataacg tgctttcaga
     8701 aaacaaccgt acaggaggat ttgaagccgt cagaaaccgt atgattatcg atctttttta
     8761 cgaaaccgga atgcgtgtat cagaacttgt ttcacttacc gattcaagtg ttgattttga
     8821 cgcccgacag ttgcgggtaa tcggaaaaag aaacaaagaa cggatcattc cgctcggaga
     8881 aacaatctgt caagacctga gagtatatca ggaaataaga aacagggtga ctgatataaa
     8941 atgcgatgca ctttttgtac gaaaaaacgg aatgaaaatg tacagcaaag ctatctataa
     9001 tattgtacac aacgaaatgg cgcagttcag caccttgaaa aaacaaagtc ctcacgtttt
     9061 gcgtcatact tttgctacca tcctgcttaa caacggagct gatataaatg cggtaaaaga
     9121 cctgctcggg catagcagtc tggctgctac ccaggtttat acccatgtaa gtttcagcga
     9181 attaaataaa atttataaac aggctcatcc acgagctatt aaaaaaggag gtcagatatg
     9241 aaatttagaa ttcaatccat caactttgat gctacagaaa agttggaagc ttacatcaac
     9301 aaaaaagcta tgaaactcag taaggtgttt gatgaaatta tgactatgga tgtgtacctg
     9361 aaggtggtta agccggaaac ggccacaaac aaggaagcag aaataaaggt gacagctcct
     9421 aacgtggaat tctttgcctc gaaaacctgc gatacttttg aagaagcagt tgatctgggg
     9481 ttggaagcaa tcgaaaagca agtgattaaa tacaaggaaa aattagctaa aaaataaaaa
     9541 attgaacaat tagttttgta tatcaaaata attttgtatt tttgcaagcc gtttgaatag
     9601 cgatattcga acggcttttt atccctgcaa aggcttagat aataagtatt tgcctcttta
     9661 gctcagctgg ccagagcacg tgatttgtaa tctcggggtc gttggttcga atccgacaag
     9721 aggctcactt ttagtaaaat agtatattag tataaaagtt gagtagtgaa atagttaatt
     9781 tttttgccaa aatggggcaa atatccgaat ttactaatta accaatcaac caaaaaaaag
     9841 ggcagttacc aaagtggcca actggggctg actgtaactc agctgtcttt c
//
LOCUS       DAORKP010000085         9837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_897_length_9837_cov_8.349622, whole genome shotgun sequence.
ACCESSION   DAORKP010000085 DAORKP010000000
VERSION     DAORKP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_9837_cov_8.349622"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..104)
                     /locus_tag="PLN75_06030"
     tRNA            complement(32..104)
                     /locus_tag="PLN75_06030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Thr,seq:cgt)
     gene            454..3729
                     /gene="carB"
                     /locus_tag="PLN75_06035"
     CDS             454..3729
                     /gene="carB"
                     /locus_tag="PLN75_06035"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HRG03708.1"
                     /translation="MDKSIKKVILLGSGALKIGQAGEFDYSGSQALKAMREEGIETVL
                     INPNIATIQTSEGVADKVYFLPITPYFVEKVIQKEKPDGILLAFGGQTALNCGTELYQ
                     SGVLARYGVQVLGTTVEAIMITEDRDLFVKKLDEIEIKTPVSHAVESLEDALAAAREI
                     GFPIMVRSAYALGGQGSGISENETEFIELCKSALSFSQQILVEESLKGWKEIEFEVIR
                     DKNDHCFTVVPMENFDPLGIHTGESIVVAPIITLSKEQIALLEDIARQVVRHIGIVGE
                     CNIQYAFNAETNDYRIIEINARLSRSSALASKASGYPLAFVATKLALGYSLDQIGEMG
                     TPNSAYVAPSVDYMIVKIPRWDLTKFEGVSREIGSSMKSVGEIMSIGQSFEEIIQKGL
                     RMIGQGMHGFVGNRDMTFDNVEDELSNPTDLRIFAIADAFEKGYTVDQIEALTKIDKW
                     FLEKLENIVRYSKVLAGFNRIEDLTEEVLNEAKRLGFSDFQIARFVENPQGTVEDDLL
                     RVRTHRQVMNVKPIVRRINTIASENPEKTNYLYFTYGAPSNSPKGRENTSYTTSTNAE
                     KMHISPPSEGTGEVVVVLGSGAYRIGSSVEFDWCSVNAVQTARNLGYQSVMINYNPET
                     VSTDYDMCDRLYFDELTLERVLDVIEIENPKGVIVSMGGQIPNNLAIKLHRQHVPILG
                     TSPLSIDRAENRHKFSAMLDSLNIDQPRWKELTSFDEIDAFVEEVGFPVLIRPSYVLS
                     GAAMNVCYSKDQMHEFLKMAARVSKEFPVVVSVFMQNAKEIEFDAVAKNGEVVEYAIS
                     EHVEFAGVHSGDATLVFPAQKIYFETMRRVKKISKQIARELKISGPFNIQYLAKNNEI
                     KVIECNLRASRSFPFVSKVLKHNFIETATRIMLDAPFRKPDSSVFDLEYIGVKASQFS
                     FSRLQNFDPILGVDMSSTGEVGCIGDDFGEAMLKSMLSVGYNIPKKGVLISSGEVKSK
                     VDLLEACKLLHEKGFELFASHGTQKFLEENGVKATDVNWPDEDGENNIRTMIAEKRFD
                     LIINIPKDITKRELTNGYIIRRSAVDYNIPLITNARLASAFINSFCSLKPDDVKIKSW
                     QEY"
     gene            complement(3811..4266)
                     /locus_tag="PLN75_06040"
     CDS             complement(3811..4266)
                     /locus_tag="PLN75_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03709.1"
                     /translation="MLLNRNIKENNKNAFYHLVIGLIFIGMTLWVYFKGENKPNGVDQ
                     FDWVYIFIFGMVGVFFSFKGLSSIFRKAYILVDEERIAIKPDETTKSETILWNDIEAI
                     RQVEKNFEILKKDGSSYIIYFSYFKYENAGDLKEAIIDMASTKGISIQE"
     gene            4468..5028
                     /locus_tag="PLN75_06045"
     CDS             4468..5028
                     /locus_tag="PLN75_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03710.1"
                     /translation="MTRKIKISSVVIFAGLMLAFTSCETIKEKLAVPIPINNVEVIFE
                     VQPAPASVPAQKAPAITAETKVLLDKTFDVNVVEEAEKLGYDLDNIIEFVITAGTIEL
                     VEPAGFDMNEFKNVKIYFDNQTNLVAQADKIQNGKITIKIVDGDLLQMLKNDQLHVII
                     TGDSLPSKRVKLKFISSYRAKIQVIK"
     gene            complement(5107..5625)
                     /locus_tag="PLN75_06050"
     CDS             complement(5107..5625)
                     /locus_tag="PLN75_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG03711.1"
                     /translation="MNLLESVNIRLRAPEPEDLELLYKWENDSSGWHTCNTRTPYSRF
                     QLKKYIASSTFDIYENKQLRLMMMEKTTSQVVGTVDLFDFDLHHSRVALGLYVAPEFQ
                     GRGYARESLKLAENYVFNFLKINQLYVQIAESNMPSRKLFENNYELHGMLKHWIKTHE
                     GFENILTYQKFR"
     gene            complement(5633..6589)
                     /locus_tag="PLN75_06055"
     CDS             complement(5633..6589)
                     /locus_tag="PLN75_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRG03712.1"
                     /translation="MMIKNFLHFNRGQKIGILIMLGLIVTSLLAGWLLPRFVQPKTAT
                     TDTSFLTEAAKFKESLVELERAKQNRYPDYDNFYKPYPKKTFEKEKYELFIFDPNSAD
                     SATLVRLGLKPYVAVNILRYRVKGGKFRKPEDFARVYGITPEKFEELKPFISFESNIA
                     KVADENFSETENQEIKPEKKNLKNGIAIELNTADTAAMLQIAGIGPSTARGIARYRNL
                     LGGYHSIEQLKEVYGMRPENFERIRSNFTIDDSQIKKINVNTASVDRLKKHPYIKTFQ
                     KAVAIYEYRRKKVKLSGIEDLKVLDELTGEDLSKLAPYLEFK"
     gene            complement(6705..8186)
                     /gene="proS"
                     /locus_tag="PLN75_06060"
     CDS             complement(6705..8186)
                     /gene="proS"
                     /locus_tag="PLN75_06060"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRG03713.1"
                     /translation="MAIEIKELTSRDTDYSQWYNDLVIKADLAETSAVRGCMVIKPYG
                     YAIWEKIQGELDRMFKETGHVNAYFPLFIPKSFLSREAEHVEGFAKECAVVTHHRLKQ
                     DPTGSGLIVDPEARLEEELIVRPTSETIIWNTYKNWIQSYRDLPILVNQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATEAEAIEEAERMLHVYADFAENFMAVPVLRGLKSANE
                     RFAGAIETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVTFVNKENKMEYVWATSWGV
                     STRLMGALIMTHSDDNGLVLPPNLAPYQVVIVPIYRNEEQRAAITEKVNELTAKLRAL
                     GISVKYDNNDNKKPGWKFAEYELKGVPVRLAMGARDLENGTVEVARRDTLTKETVSFE
                     GIDTFVGHLLKEIQDNIYQKALNYRSSMTREINSYDEFKVEIEKGGFLLCHWDGTPET
                     EEKIKEETKATIRCIPLTGDKTPGLCMVTGKPSVQRVVFARAY"
     gene            complement(8327..8641)
                     /locus_tag="PLN75_06065"
     CDS             complement(8327..8641)
                     /locus_tag="PLN75_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRG03714.1"
                     /translation="MKQVTFDFEKIESIDDFYREAVEKMDLPDYFGYNLDAMWDCLTG
                     EIELPIEIRFINLTSEQLDEFVEIILLFEDAAFDMDEDLFFEYSLKEDEADLLLTGDE
                     TE"
     gene            complement(8638..9138)
                     /locus_tag="PLN75_06070"
     CDS             complement(8638..9138)
                     /locus_tag="PLN75_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRG03715.1"
                     /translation="MQKKSSTIIIFLLLIITGILLYNTFIQKNDVKNDVKTGNVTKKT
                     TNKPSVITTDNQEIDKLTREEVVVPYVKENGRLPDYYITKRQARQHGWIASEGNLCDV
                     LPGRAIGGDIFTNREKRLPTRKGRTYYEADLNYKCGNRNADRLIFSDDGLIFVTHDHY
                     KTFEKQ"
     gene            9321..>9837
                     /locus_tag="PLN75_06075"
     CDS             9321..>9837
                     /locus_tag="PLN75_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRG03716.1"
                     /translation="MRFDELPLCDAVLDGLDAMNFTEMTPVQEQAIPVILEKRDLIAC
                     AQTGTGKTAAFLLPLINNLYTEPRVEHAVNAIIMSPTRELAQQIDQQMEGFSYFVPVS
                     SVAVYGGSDSQAWDVQRNGLQKGADVVIATPGRLISHLNLSEIDLSHVKYFILDEADR
                     MLDMGFFDDIMM"
BASE COUNT         2704 a         2021 c         2160 g         2952 t
ORIGIN      
        1 aaatgctttg tagtaagggt tttggagggt gagccttttt ggggactcga accccagacc
       61 tattcattac gaatgaattg ctctaccgac tgagctaaaa aggcttgaaa aaaaaggctt
      121 gattaatggc ctaataaatg gattgcaaag atagaactta aatttctttt ttgtaaaata
      181 ttttcaaaaa ttttgaccgg gatattaaat aaaagcccta actttgcata aattatttat
      241 acaggtattt tctaatcaat aagataaatg tattctaagt tgtgtaagcc tgcatcagat
      301 gaggcaactg ttatacgggg tatcggcaaa ccggtgtatt tatcctcaaa acaacttatt
      361 tttttttata aatcggaaaa ataaagacaa tcagtctgaa tttcttccga ttttttttgt
      421 gtgtataaaa aactgaaaaa aataaatata caaatggaca aatcaatcaa aaaagtaatt
      481 ttactgggtt caggtgctct gaaaataggg caggcgggcg aatttgacta ttccggttcg
      541 caggccctca aagcgatgcg cgaagaaggc atcgaaaccg tattaatcaa tccgaacatt
      601 gctaccattc agacttcgga aggggtggcc gataaagtat atttcctacc cattacacct
      661 tattttgtcg aaaaagtaat tcagaaagaa aaaccggacg gtatcctgct tgcttttggc
      721 ggacaaacag ctctcaattg cggcactgaa ctgtatcaga gcggtgtact tgccaggtat
      781 ggcgtacagg tattgggaac aacggtagaa gccattatga ttaccgaaga ccgtgatttg
      841 tttgtaaaaa aactggatga aattgaaata aaaactccgg tttcgcacgc cgtggaaagt
      901 ctggaggatg cgctcgccgc tgcccgtgaa atcggatttc cgattatggt tcgttcggct
      961 tacgcacttg gcggacaagg gtcgggtatt tcggagaatg aaaccgaatt tatagaactc
     1021 tgtaaaagcg ctctctcgtt ttcgcaacaa atactggtgg aagagagtct gaaaggatgg
     1081 aaagagattg agtttgaagt aatcagggat aaaaacgatc attgttttac cgttgtaccg
     1141 atggagaatt tcgatccgct gggcattcac acgggcgaaa gtatagtggt tgctccaatc
     1201 attactttgt cgaaagaaca gattgctttg ctcgaagaca ttgcccgtca ggtagttcgt
     1261 catattggta ttgtgggcga atgcaacatt cagtatgcct tcaatgccga aacaaacgat
     1321 taccgcatca tcgaaatcaa cgcccgcctc agtcgctcgt cggcgctggc ttccaaagca
     1381 tcgggttatc cgctggcatt cgtggccacc aagctggcgc tgggttattc gctggatcag
     1441 attggcgaaa tgggaacacc aaattcggcc tacgtggcac catctgtaga ttacatgatt
     1501 gtgaaaattc cacgctggga cctcaccaaa tttgaaggag taagccgcga aatcggatca
     1561 tccatgaagt cggtgggcga aattatgtcc atcggacaat cgtttgaaga aattattcag
     1621 aaaggactgc gcatgatagg gcaggggatg cacggatttg tagggaatcg tgatatgact
     1681 ttcgataatg tggaagatga gctttccaat cctaccgatt tgcgcatttt tgccattgca
     1741 gatgctttcg aaaaaggata tacggtggat cagatcgaag cgcttacaaa aattgacaag
     1801 tggtttcttg aaaaacttga aaatatagtg cgctacagca aggtacttgc cggattcaac
     1861 cggattgaag accttaccga agaagtactt aacgaagcca aaaggctcgg tttcagcgat
     1921 tttcagatag cccgttttgt ggaaaatcca cagggaactg tagaagatga tttgttacgc
     1981 gtacgaacac atcgtcaggt tatgaacgta aaaccaattg ttcgccggat aaacaccatt
     2041 gcttcagaaa atccggaaaa aaccaactat ttgtatttta cttacggagc cccctctaat
     2101 tcccccaaag gacgagaaaa cacgtcctac accacaagta ctaatgctga aaagatgcat
     2161 atttcccctc cttctgaggg tactggggag gttgttgttg tgttgggttc tggtgcttac
     2221 cgcatcggtt cgtcggtaga gttcgactgg tgctcggtga atgccgttca gactgcccgg
     2281 aatctgggct accagtctgt aatgattaat tacaaccctg aaacggtttc gaccgattat
     2341 gacatgtgtg atcgccttta tttcgacgaa ttaacgctgg aacgcgtcct ggacgtgatt
     2401 gaaatagaaa atccgaaagg agtaattgtg tccatgggcg gacaaattcc gaataacctc
     2461 gctatcaagc tgcatcgtca gcacgtaccc attttgggaa cttcgccact ttcaatagat
     2521 cgcgctgaaa acaggcataa attctcggcc atgctcgaca gcctgaatat tgatcagccc
     2581 cgttggaagg aactcaccag tttcgatgag atagatgcct ttgtggaaga agttggattt
     2641 ccggtactta tccggccttc ctatgtactc tcgggcgccg ccatgaatgt atgttacagt
     2701 aaagatcaga tgcatgaatt cctgaagatg gcagccaggg tttccaaaga atttccggtt
     2761 gtggtgtctg ttttcatgca gaatgccaag gagattgagt ttgatgcggt tgccaaaaac
     2821 ggtgaagtgg tggaatatgc catttccgag cacgttgaat ttgcaggtgt tcactcgggc
     2881 gatgccacac tggtgtttcc ggctcagaaa atttacttcg aaaccatgcg ccgggtgaaa
     2941 aaaatctcaa aacaaattgc ccgtgaattg aaaatcagcg gtccgttcaa cattcagtat
     3001 ctggccaaaa acaatgaaat taaggtaata gagtgtaacc tgagagcttc ccgttctttc
     3061 ccgtttgttt ccaaagtgct gaaacataat tttatcgaaa cagccacgcg catcatgctc
     3121 gatgcccctt tccgcaaacc ggacagctcg gtattcgacc tggaatacat cggcgtgaaa
     3181 gcatcgcagt tttcattttc ccgattgcag aatttcgacc ccattctggg cgtggatatg
     3241 tcgtcgaccg gcgaagtggg ctgcataggc gatgattttg gcgaagcaat gctcaaatcc
     3301 atgctttcag ttggctacaa cattccgaag aaaggtgttt tgatttcatc aggcgaagtc
     3361 aaatcaaaag tggatctgct tgaagcatgc aaattgcttc acgaaaaagg ctttgaactc
     3421 tttgccagcc atggtactca aaaatttctg gaagaaaacg gggtgaaagc cacagatgta
     3481 aactggcccg acgaagacgg tgaaaacaac atccgcacca tgattgccga aaaaagattt
     3541 gacctgatca tcaatatccc gaaagacatt acgaaacgtg agctgaccaa cggttatatc
     3601 atccgccgca gcgcagtgga ttataacatt ccgctcatta ccaatgcgcg actggcttcg
     3661 gcttttatca attcattctg cagcctgaag cccgacgatg tgaaaataaa gagctggcag
     3721 gagtattaag ttcataatca acttagtatt aaataaaaaa atcccttcaa agtttaaaag
     3781 aagggatttt ttgtattcag tgtaagcgtt ttactcctga atagaaatac cttttgttga
     3841 tgccatatcg ataatggctt ctttcaaatc gccggcattt tcgtatttga agtaggagaa
     3901 ataaatgatg taggaggacc cgtctttttt cagaatttcg aaattctttt cgacttgtct
     3961 gatggcctca atgtcattcc ataaaatggt ttcagacttg gttgtttcgt ccggcttgat
     4021 tgcaattcgc tcttcgtcca ccagtatgta ggctttccgg aaaatggaac ttaaaccttt
     4081 gaacgaaaaa aaaactccaa ccatcccgaa aataaaaata tacacccagt caaactgatc
     4141 cacgccgttc ggtttgtttt cgcctttgaa gtacacccag agtgtcatac cgataaaaat
     4201 aaggccaatg acgaggtgat aaaatgcatt tttattgttt tctttgatat tgcggttgag
     4261 taacatattg ctgcaatttt tatttgttct gacggcagca aaggtaataa aacgattgat
     4321 tttgtgcaaa aaaccttcag aataacagaa attaatccct tttttgagat attttctttt
     4381 ttttctcaaa aacaaatttc tgaatttgat tgttattcta taaatttgat aactttacaa
     4441 actcaaacaa agctaaaaaa taccactatg actcgaaaaa ttaaaatctc ctcagttgtt
     4501 attttcgccg gattaatgct ggcatttacg tcgtgcgaaa ctattaagga aaaactggct
     4561 gtacccattc cgataaacaa cgttgaagtt atttttgaag ttcagcccgc tccggctagt
     4621 gtccccgcgc aaaaagcacc tgctattact gctgaaacca aggtacttct ggataagacc
     4681 tttgatgtaa acgttgtgga ggaagctgaa aaacttggct acgatctgga taacatcatt
     4741 gaatttgtga ttacggccgg aaccattgaa ctggtggaac ctgccggctt cgatatgaat
     4801 gaattcaaaa atgtgaaaat ttattttgat aatcagacta atctggtagc tcaggccgat
     4861 aaaattcaaa atggaaaaat taccatcaaa attgttgacg gcgatttact tcaaatgttg
     4921 aaaaatgatc agttgcatgt aattattacg ggtgattcgt tgccttccaa acgtgtgaaa
     4981 ctgaaattta tttcgagtta cagggctaaa attcaggtga ttaaatagag aagttttcag
     5041 tttataagct actgtgaaaa aagatgctga ttttgtcacc aaaatcagca tctttcattt
     5101 ttcttactac ctgaattttt ggtaggtgag tatattttca aaaccttcgt gcgttttaat
     5161 ccagtgcttc agcatgccgt ggagttcgta gttgttttcg aaaagttttc ggctgggcat
     5221 gttgctctca gctatctgca cgtagagttg attgattttc agaaagttga aaacgtaatt
     5281 ttcagccagt ttcagacttt cacgggcata acccctgccc tgaaactcgg gtgccacgta
     5341 aagccctaat gccacccggc tgtgatgcag gtcgaaatca aacaaatcta ctgtcccaac
     5401 aacctgagaa gtggtttttt ccatcatcat tagccggagc tgtttgttct cataaatatc
     5461 aaatgtggag gatgctatgt atttttttag ctgaaaccgt gagtagggtg tgcgtgtgtt
     5521 gcaggtgtgc catcccgaac tgtcgttttc ccacttgtag agtaactcca ggtcttcggg
     5581 ctccggtgcg cgcaatctta tattgactga ttccaacagg ttcattttga gtttatttaa
     5641 attccagata cggagctaat ttgcttaaat cttctcctgt aagttcatcg agtactttca
     5701 aatcttctat tccgctgagt ttcacttttt tgcgtcggta ttcgtaaatg gccacagctt
     5761 tctggaaagt tttgatataa ggatgctttt tcagtcggtc cacgctggcg gtgttcacgt
     5821 taattttttt tatttgcgag tcatcaatcg taaaatttga cctgattcgt tcgaaattct
     5881 caggtcgcat tccgtatact tctttcagct gctcaatgga gtggtagcct cccaaaaggt
     5941 tccgatatcg ggcaattccc ctggcagttg aaggtccgat acctgctatc tgaagcattg
     6001 cagcagtatc ggctgtattc agttctattg caataccatt tttaagattc tttttctccg
     6061 gttttatttc ctgattttcc gtttcagaaa aattttcatc cgctactttc gctatatttg
     6121 attcaaatga aataaaaggc ttcagttctt cgaatttttc gggagtgatg ccgtaaaccc
     6181 gggcaaaatc ttccggtttt ctgaattttc cacctttgac tctgtatcgt aatatgttta
     6241 cagctacgta aggctttaat cccagtcgaa ccaacgtggc tgaatcggca gaattgggat
     6301 caaaaataaa aagttcgtat ttctctttct caaaagtctt tttcggatac ggtttgtaaa
     6361 aattgtcgta atccgggtaa cggttttgtt ttgctcgttc cagttcaaca aggctttctt
     6421 tgaattttgc ggcttctgtc agaaatgagg tatcggttgt tgctgttttc ggttgtacaa
     6481 aacgaggcaa caaccaacct gccagcaaag atgtcacaat cagtcccagc ataattaaaa
     6541 taccgatttt ctgcccccgg ttgaaatgga ggaaattttt tatcatcatt aagctcttaa
     6601 agtctccacg agatatgtat ttgtatttta atctacatca atgttgccgg attcgatttt
     6661 agaattctct gaaagcctcc ccgtcggaga gcctggagtg gcttctaata cgcccgggca
     6721 aataccacgc gctgtaccga aggcttaccg gtaaccatgc ataatcccgg agttttgtcg
     6781 cctgtgagcg gaatgcagcg gatggtggct ttggtttctt cctttatttt ctcttctgtt
     6841 tcgggcgtac cgtcccagtg gcaaagcagg aatcctcctt tttcgatttc cactttaaac
     6901 tcatcatacg aatttatttc gcgggtcatg gaggagcggt agttgagtgc cttctggtaa
     6961 atattatcct gaatttcttt cagcaaatgg ccgacaaaag tatcgatacc ttcgaaagat
     7021 acggtttcct tggtgagtgt gtcgcgtcgg gctacttcta cagtaccgtt ttcgaggtca
     7081 cgggcaccca tagccaggcg tacaggtacg cctttcagtt catattccgc aaatttccag
     7141 cccggttttt tattgtcgtt gttgtcgtat ttcacactga tgcccaaggc acgtagtttt
     7201 gccgttaatt cattcacttt ttcggttatg gcagcgcgtt gttcttcatt cctgtaaata
     7261 ggtacgataa ctacctgata tggagccagg tttggaggca gtacaagccc gttgtcatcg
     7321 gagtgagtca tgatgagtgc tcccataagc cgggtagaaa ctccccagga tgtagcccac
     7381 acatattcca ttttattttc tttattcaca aaggtcacat caaatgcttt ggcaaagttt
     7441 tgtccaagga agtgtgaagt gcctgcctgg agggcttttc cgtcctgcat aagtgcttcg
     7501 atgcagtatg tttcgatggc accggcaaat cgctcgttgg ccgatttcaa tccgcgcagt
     7561 accggaacgg ccatgaagtt ttcggcaaaa tcggcgtaca catggagcat tctttcagct
     7621 tcctcgatgg cttctgcttc ggtagcgtgg gcagtgtgtc cttcctgcca gaggaattcg
     7681 gccgttcgaa ggaaaagccg ggttcgcatt tcccagcgta ctacgttggc ccactgatta
     7741 accaggatag gtaaatcgcg gtaagattgg atccagtttt tatacgtgtt ccagatgatg
     7801 gtttcggagg tggggcgcac gatgagttct tcttccagac gggcttccgg atctacaatt
     7861 aatccgctgc cggtggggtc ttgtttcagg cggtgatgcg tcaccacggc acattctttg
     7921 gcaaatcctt ccacgtgttc ggcctcacgg ctcaggaatg atttggggat aaaaagcgga
     7981 aaataagcat tcacatggcc ggtttcctta aacatccggt cgagttctcc ctgaatcttt
     8041 tcccagatgg cgtatccgta aggtttaatc accatacagc cacgtactgc cgaagtttcg
     8101 gccagatcgg cttttatcac taaatcattg taccattggg agtaatctgt atcacgcgaa
     8161 gtgagttctt ttatttctat tgccatattc ttgtgaaaat ttttatccgt aatctgccgg
     8221 ataataatca ttatatcagt atattaagtt tttgcgtgca gttatttctg aaaaaacaga
     8281 ctgcaaagat agttaatttt cggaaaatat gccaaccgga atcgggctac tctgtttcat
     8341 caccggtcaa cagcaaatct gcctcatcct ctttgagcga atattcaaaa aataaatctt
     8401 cgtccatatc gaaagcggca tcttcaaaca gaagaattat ttcaacgaat tcatccaact
     8461 gctccgatgt aagattgata aacctgattt caatcggaag ttcaatttca ccggtgaggc
     8521 aatcccacat tgcatcgagg ttatagccga aataatcggg caaatccatt ttttcaacgg
     8581 cttcacggta gaaatcgtct atggattcta ttttttcaaa atcaaaagtt acctgtttca
     8641 ttgtttttcg aaggttttat aatggtcgtg agtaacaaaa atcagtccgt cgtcggagaa
     8701 aataagcctg tcggcattgc ggtttccgca cttgtagttc agatcggcct cgtagtaggt
     8761 tcggccttta cgtgtcggaa gtcgcttctc gcgattggtg aaaatgtctc cgccgatggc
     8821 tcttccgggt agtacgtcac acagattgcc ttctgaggct atccagccat gttgtctggc
     8881 ttgtcgcttg gtgatgtaat aatcggggag acggccgttt tctttcacat acggcaccac
     8941 tacttcttca cgggtaagtt tgtcaatctc ttgattgtca gtagttatta ccgaaggttt
     9001 atttgtcgtc ttctttgtta cattcccggt ttttacatca ttttttacat catttttttg
     9061 aatgaaagta ttgtaaagca aaataccggt gataatcaac agcagaaaga tgatgatggt
     9121 ggatgatttc ttttgcattg agtatatttt tctacaaata tagttaactt tttatgattc
     9181 ttacatttta tgatataaat ctgatgtgtc tgtttagtgt aaactgaagg tcggaatctt
     9241 aaataacctg ctgtaaaaca tcatttttca aaattttagt agtacttttg tgaaaaaata
     9301 agaaaataaa ttcctataaa atgagatttg atgaacttcc gctgtgtgat gcggtgctgg
     9361 acggacttga cgccatgaat tttaccgaaa tgactcccgt tcaggagcag gcaatacctg
     9421 ttattttgga aaagagagat ttaatagcct gtgcacaaac cggtacaggc aaaacagctg
     9481 cgtttttgct tcctcttata aataaccttt acaccgaacc ccgcgtggaa catgctgtaa
     9541 atgccattat catgtcgcct actcgcgaac tggcacagca aatcgatcag cagatggaag
     9601 gtttttcgta ctttgtacca gtatcttcgg tagcggtgta cggaggcagt gattcgcagg
     9661 cttgggatgt tcagcgtaac ggactgcaaa aaggagccga tgtggtgatt gcaacgcccg
     9721 gcagattaat ttctcatttg aatttatcgg aaattgacct ttcgcatgtg aaatatttca
     9781 tactggatga agccgaccgg atgctcgata tgggattttt tgacgatatc atgatga
//
LOCUS       DAORKP010000086         9793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_908_length_9793_cov_9.420415, whole genome shotgun sequence.
ACCESSION   DAORKP010000086 DAORKP010000000
VERSION     DAORKP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_9793_cov_9.420415"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..2313)
                     /gene="recQ"
                     /locus_tag="PLN75_06080"
     CDS             complement(127..2313)
                     /gene="recQ"
                     /locus_tag="PLN75_06080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRG03717.1"
                     /translation="MTIHSDLTAQLKKYFGFGTFKGNQEAIIRNVMSGKDTFVLMPTG
                     GGKSLCYQLPALLLEGTAIIVSPLIALMKNQVDAMRNFSEEDGIAHFLNSSLTRSAID
                     VVKEDVLSGKTKMLYVAPESLTKEENIELLRQVKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIISEIGTAPIIALTATATPKVQHDIQKNLTMLEATVFKSSFNRPNLYYEVRAKN
                     KDVEKEIIKYIRSRPGKSGIIYCLSRKKVEELAETLQVNHISVLPYHAGMDATQRTMN
                     QDSFLMEKVEVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRGGRDGGEGEC
                     ITFYSYKDLDKLRKFMQGKPIAEQEIGNQLLLETQAYAESSVCRRKLLLHYFGEEYPH
                     DNCGSCDNCMKPKKIIEGQELLLLVLETVQQLKEKFKAEHIVDILKGNETSDITSYQH
                     DELENYGVASDEEEKLLHAVIRQAMIAGYISKDIETYGVLKLTPAGKEYLKKPLPFPI
                     VKDTEFEEDDEEAAGRTVGGTSAADDVLFSILKDLRKKLSKKLEVPPFVIFQDPSLEA
                     MATTYPVTMEELQNIPGVGAGKAKRYGEEFLKVIKEHVKENDIIRPEDLRVRTVAKKS
                     QLKVSIIQAIDRKIALDDIALSKGLDFNEMLEELEAIVYSGTKININYFLEEVMDPDK
                     VDEIFDYFNNAETDKLNEALDELGEDDFTETEIRLVRIKFLSEVGN"
     gene            complement(2550..3782)
                     /gene="clpX"
                     /locus_tag="PLN75_06085"
     CDS             complement(2550..3782)
                     /gene="clpX"
                     /locus_tag="PLN75_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRG03718.1"
                     /translation="MAKNTKKCNFCGRSESETGFLILGIEGVHICNECALQAAEIVNE
                     TIKDKSGLPKLDKEKVPKPKEIKTFLDQYVIGQEEAKKFLSVAVYNHYKRLMQDKHND
                     DVEIEKSNIIMVGYTGTGKTLLAKTIARKLDVPFTMVDATVLTEAGYVGEDIESIITR
                     LLQVADYNVNLAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSMVNV
                     PPQGGRKHPEQKFIQVNTQNILFICGGAFDGIEKKIAQRLNTRVVGYGASLDSENIDR
                     NNMLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDREALRRILTEPKNSIIKQYVKLF
                     KMDHMELVFEPEVLDYIVDKAIEFKLGARGLRSITETIMMDAMYESPSKKANKLVVTL
                     EYAHSKMDKMSAHRLKIA"
     gene            complement(3868..4545)
                     /gene="clpP"
                     /locus_tag="PLN75_06090"
     CDS             complement(3868..4545)
                     /gene="clpP"
                     /locus_tag="PLN75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRG03719.1"
                     /translation="MEKRNDFRKYATKHLGMNGLALDQYAQITDSYISPSILEERQLN
                     VTQMDVFSRLMMDRIIFLGTAVDDYAANVIQAQLLYLDSSDPGKDISIYLNTPGGSVY
                     AGLGIYDTMQFISSDVATICTGMAASMGAVLLVAGTKGKRSALKHSRIMIHQPSGGAQ
                     GVAADMEINLREMMKLKKELYQIIADHSGKTYEQIEKDSDRDFWMTSQEAMEYGMIDR
                     VLIKEKK"
     gene            complement(4797..6152)
                     /gene="tig"
                     /locus_tag="PLN75_06095"
     CDS             complement(4797..6152)
                     /gene="tig"
                     /locus_tag="PLN75_06095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRG03720.1"
                     /translation="MNISKKDIDQINSVITISVSKEDYAEKVEKSLRDYRKKANMPGF
                     RPGNVPIGLIKKMYGKAVKAEEINNLVSEGLVNFIEENDIQILGQPLPNEKSEFDFDT
                     QDDFDFHFDIGIAPQFDVNLNDMPDVPYYEISVSAEMIDNQIKSYASRFGKYEQVDSV
                     DEKDMVKGNLAELENGEVKAGGITVADAVLTPAYMKDEEQKNLFTGKSKGDKIVFNPQ
                     KAFENETEISSLLKIRKEEAKDLTSDFEIEITGITRYQEGEVNQELFDKVYGEGVINS
                     EDEFREKIKENIKESLIADSDYKFNIDVREALIKQFSNLEFPETFLKRWLIDNNKDMT
                     PEKVEEDYPKMIEDLVWHLTKDKIAKANEIKVELPDVEEYARKVAKAQFAQYGMVGLG
                     DEILDNYVKDMMKKEETVRNFIDRAAEEKVLAVIRQKMNLDKKEISIEDFNKMFEQTQ
                     G"
     gene            6465..9143
                     /locus_tag="PLN75_06100"
     CDS             6465..9143
                     /locus_tag="PLN75_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRG03721.1"
                     /translation="MISAYGESPEITSLKVEMQTNPKGLSTLSPRFSWQINADKPNLK
                     QTSYRIQVSSSEKDLLKEKNLLWDSGQIESEVSQLIPYSGLKPGSGTQYFWRVRVGTN
                     QGKSKWSKAGSWSTALLADSDWKARWIGEDTLSNPGETSSKNTRLAARYLRKTFTADK
                     KVKRAVLYISGLGMYEAYINGLRISEDVFSPTPSWYPERVYYNTYDVTHLLKNGVNVL
                     AVKLGNGRFFGMRENKTMMFGLPRLLAQLEMENTNHQKTTIITDQSWKITSRGPIVSN
                     NEFDGEEYDARLEMKGWNSPEFDDAGWETAEIMKNPGGKLTAQPNPNIAVQDHITPLR
                     IIKTTGNKYVLDMGQNMVGWLKVNLKGKKDKPLKMRFAEILKKDSTLYLDNIRSAKVT
                     DIYTPATDGMFTWEPSFTYHGFRFVEIEGLDYEPSVTDFEGKVIYDRMTTSGSFETSS
                     EMINTIYKNAFWGIRGNYRGMPTDCPQRDERMGWLGDRATGCFGEAFVFDNSQLYSKW
                     IQDIEDSQSPEGSISVVSPRYWTIYNDDVTWPAAYFYAAQMLIRQFGDTKPIIKHYDS
                     MKKYLEQIQRISMKDYIITKDTYGDWCMPPEKQELIHSKDPARKTDGAILSTTMYYQL
                     LNMMCEFAEISSNNQDIESYRNLASKIKQAYNEKYFDKNTAQYGNNTVTANILSLRLG
                     LVPAGYEQRVFENIVKKTETEFNGHVSTGVLGIQHLMRGLTEFGKSDLAFKIATNDTY
                     PSWGYMAQKGATTIWELWNGDTADPSMNSGNHVMLLGDLLIWYYEDLAGIKNAPESNG
                     YKKLLMQPVFPNNLHHVKASYQSVYGEIKSEWTRKEGLFDWKITIPGNASAIVRIPKE
                     ITLNQLNTNEIISQLKHADYTEIEVGSGHYSFTGNY"
     gene            9234..>9793
                     /locus_tag="PLN75_06105"
     CDS             9234..>9793
                     /locus_tag="PLN75_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRG03722.1"
                     /translation="MKNILTFLSFIIITSFSVHSQGISFEENHDLNAALAKAKTENKL
                     IFIDAYAVWCGPCKVMARDIFPLKEVGDFFNANFVNLKLDMEAKENINIAKKYEVQAY
                     PTYLFLNADGELVHKGLGSMPAAQFIEVAKVAADTENNYAALSKKIKNGDRSLATVKK
                     FITQNPYDTGNGALVDSYFQTLSENQ"
BASE COUNT         2722 a         2091 c         1917 g         3063 t
ORIGIN      
        1 gatgtattga taatcaatta gatataaaat tagtttcttt gtgtaacttg gtgttttagt
       61 gtctttgtgg caaaaacaag gttttcggag cagactcatt cttggaacta tttaaaaatt
      121 cagttctcaa tttccaactt cagataaaaa tttaattcgt actagacgga tttctgtttc
      181 ggtaaagtca tcttcaccta gctcatccaa tgcttcattc agtttatcgg tttcggcgtt
      241 attgaaatag tcgaatattt catccacctt atctggatcc atcacttctt caaggaaata
      301 attgatgtta attttggttc cggagtaaac gatggcttcc agttcttcga gcatttcgtt
      361 gaaatccagt cctttagaaa gtgctatatc gtcaagggct atttttcggt caatagcctg
      421 aatgatagat actttcagct gagatttttt ggctacagtg cgtaccctca agtcttccgg
      481 ccgtataata tcattctctt taacatgctc tttaatcact ttcaggaact cttcgccgta
      541 tcgtttggct tttcctgcac ctactcccgg aatattttga agttcttcca ttgttacagg
      601 ataggtggta gccattgcct cgagcgatgg atcctggaag attacgaacg ggggaacttc
      661 cagttttttg gagagtttct tacgcagatc tttcagaatg gagaacagca catcgtcggc
      721 tgcactggtt ccaccgacag ttcttcctgc tgcctcttca tcgtcttctt cgaattcagt
      781 atctttgaca atggggaaag gcagtggttt cttcaggtac tctttgcctg ctggcgtaag
      841 tttgagtacg ccgtatgttt caatatcttt acttatatag cctgcaatca ttgcctgccg
      901 gattacggcg tgcaggagtt tttcttcttc atcggaggct actccgtaat tttcaagctc
      961 atcgtgctgg taggatgtga tatccgatgt ttcattgcct ttcagaatgt caacgatgtg
     1021 ttctgcttta aacttttctt tcaattgctg aacggtttca agaactaaca ataacagttc
     1081 ctgaccttca attattttct ttggtttcat acaattatcg caacttccac aattatcgtg
     1141 tgggtattct tctccaaaat aatgtaataa taattttctt ctgcataccg atgactcggc
     1201 gtatgcctga gtttccagta ataattgatt gccgatttcc tgttcggcaa tgggtttacc
     1261 ctgcataaat ttccgtagtt tatccagatc tttataagag taaaatgtga tgcattctcc
     1321 ttcaccgcca tcccgtccgc ctcttccggt ttcctgatag taaccttcga gacttttcgg
     1381 tatatcgtaa tggattacgt atctcacatc gggtttatct attcccatcc cgaaggcaat
     1441 ggtagcaaca atgacctcca ctttttccat caggaaacta tcctgattca tagttcgctg
     1501 agtggcatcc attcctgcgt ggtaaggcag tacagagatg tgattcacct ggagtgtttc
     1561 ggccagttct tccacctttt tacggctcag acaatatatg attcctgatt taccgggtct
     1621 tgaccggatg tatttgataa tttctttttc tacatcttta tttttagccc ttacttcgta
     1681 atacagattc ggccggttga aagacgattt gaagacagtg gcttcgagca ttgtcaggtt
     1741 tttctgaata tcgtgctgta cttttggagt agctgtagca gtgagtgcta tgataggagc
     1801 agtacctatt tcgctaatta tagggcgaat gcgtctgtat tccggacgaa aatcgtgtcc
     1861 ccactcggat atgcaatgtg cttcgtctat agcatagaac gatatcttca cctgccttag
     1921 taactcgata ttttcttcct tggtaagtga ctcaggtgct acataaagca tctttgtctt
     1981 gcctgaaagc acatcttctt ttaccacatc aatggccgaa cgtgtaagtg atgagttcag
     2041 aaaatgagca attccgtctt cttcgctgaa atttcgcatg gcatccacct gatttttcat
     2101 cagggctatc agaggcgata cgataatggc ggtaccctcc agcaataaag ccggtaactg
     2161 gtaacacaat gattttccac ctcctgtggg catcagtaca aaagtatcct tgccgctcat
     2221 tacattgcgt ataatggctt cctggttgcc tttgaaggta ccaaagccaa aatacttctt
     2281 taattgagca gtcaggtcgg agtgaattgt catttttatt ttttcgactg ttgaatgaaa
     2341 acgaaattac agtatttttt tatatctgac aaaaaatatc aaagaataat ttgatgattt
     2401 attttgtttt tggatttaac aaaattataa acagttttct attatacaag tatatttaca
     2461 aaaaataatc agactgcttt tccattatcc ggctaacagt ctgattatgt atgctttgtg
     2521 atatccaatt ttttaataac aaatattttt taagctattt ttagtctgtg agcactcatc
     2581 ttgtccattt tggagtgtgc atattctaaa gtaacgacaa gtttatttgc ttttttggac
     2641 ggcgactcgt acatggcatc catcataatt gtttctgtaa tggatcttaa tccccgtgcg
     2701 ccgagtttga attcgattgc tttatctacg atataatcaa gcacttcggg ttcaaaaacc
     2761 agttccatat gatccatttt gaaaagtttg acatattgct tgatgattga atttttcggt
     2821 tcggtgagta ttcttcgcag tgcttctctg tcaagcggtt cgagataagt aagaatgggc
     2881 agacgaccga tgatttcggg tatgagtccg aatgatttga ggtcctgtgg ggcaatatac
     2941 tgaagcatgt tgtttcggtc aatgttttca gagtcgagcg aagcgccgta acctaccacg
     3001 cgggtattca atctttgtgc aatttttttc tctataccgt cgaatgcacc tccgcaaata
     3061 aacaagatat tttgggtgtt tacctgaatg aatttttgtt caggatgctt gcggcctccc
     3121 tgtggcggta cgtttaccat cgatccttcc agaagtttta gaaggccctg ctgaactcct
     3181 tcgccgctca catcgcgggt gatgctcgga ttatcgcttt ttcgggctat tttatctatt
     3241 tcatcaataa aaacaatgcc tctttcggcc aggtttacgt tgtaatcagc cacctggagc
     3301 agacgtgtaa taatgctttc gatgtcttcg cccacataac cggcttcggt gagtaccgta
     3361 gcatcaacca tcgtgaaggg cacatccagt ttccgagcta tggttttggc aagcagggtt
     3421 ttgccggtac cggtgtatcc taccatgatg atatttgatt tctcaatctc cacatcatcg
     3481 ttgtgtttat cctgcatcag ccttttgtag tgattatata ccgcaacgga aagaaacttt
     3541 ttagcttctt cctgcccgat cacatactga tccagaaaag ttttgatttc ttttggtttt
     3601 ggtacctttt ccttatcgag ctttggaagt ccggatttat ctttaatagt ttcatttaca
     3661 atttcagctg cctgcagggc acattcgttg caaatgtgaa ccccttcaat accgagaatg
     3721 agaaaaccgg tttcagattc ggacctgccg cagaaattgc atttctttgt attttttgcc
     3781 ataaaattta atgtgctaat gtgccaatgt gctaatgtgc caataaacta tcgaattagc
     3841 acattggcac atggcattat tggcttatta ctttttttct ttaataagta cccggtcaat
     3901 cataccatat tccatggctt cctgggaggt catccagaaa tcacgatccg agtctttttc
     3961 tatttgctcg taggttttcc ccgaatgatc ggcgataatt tgatagagtt cttttttcag
     4021 tttcatcatt tcacgcaggt tgatttccat atcggctgcc actccctgag caccaccgct
     4081 gggttggtga atcatgatgc gtgaatgttt cagtgccgaa cgtttccctt tggtacctgc
     4141 cacgagcaat acagcgccca tcgaagcggc cattccggtg caaatggtgg ctacgtcgct
     4201 gctgataaac tgcatggtgt cataaatacc taatccggca tatactgagc caccgggcgt
     4261 attcaggtag atggaaatat ctttgccggg atccgatgaa tcaaggtaaa gtaactgagc
     4321 ctgaatgaca ttggctgcat aatcatctac ggctgtacca agaaaaatta ttctgtccat
     4381 catcaatcgg gagaaaacgt ccatttgtgt cacattcaac tgacgttctt ccaaaattga
     4441 tggtgaaata taactgtctg taatttgtgc gtattggtct aacgccagtc cgttcattcc
     4501 cagatgcttg gttgcatatt ttctgaagtc gtttcttttt tccatattgt ttttgtaaat
     4561 tttaattttt catcaattgt tgcgaatatc ctgccaatct gtaaaacatg ccaaattgtc
     4621 atattattta cccgaatgca gctatagcct gatatacaaa ggtacatttt ttttgttaaa
     4681 acactacttt gcagccgatg aaaaaatatt ttcttaaaat aacgggttta taattattta
     4741 aaaaagcgaa atcataaaag atttcgcttt ttctgttatt ggaatggaaa aaagaattat
     4801 ccctgtgtct gctcaaacat tttattaaaa tcttcaatcg aaatttcttt tttgtccaga
     4861 ttcatcttct gacgaattac tgcgagtact ttttcttcgg ctgcgcggtc aatgaaatta
     4921 cgtaccgttt cttccttttt catcatatct ttcacgtagt tatcaagtat ttcatcgccc
     4981 aaacctacca tgccgtactg agcaaactgt gctttggcca cttttctggc gtattcttcc
     5041 acgtcaggaa gttccacttt tatttcgttt gcttttgcaa ttttgtcttt tgtcaggtgc
     5101 caaaccaggt cttctatcat tttcggataa tcttcttcca ctttttcggg tgtcatatcc
     5161 ttgttgttgt caatcaacca ccgtttcagg aatgtttcgg ggaattccag attactgaat
     5221 tgttttatca gcgcctcacg aacatcgata ttaaatttat aatcgctgtc ggctatcagg
     5281 ctttctttga tattttcctt tattttttcc cggaattcat cttcggaatt aattacgcct
     5341 tcgccgtaca ccttgtcgaa taattcctga ttaacttcac cttcctgata gcgtgtaata
     5401 ccggtgattt caatttcaaa gtcagaagtc agatcttttg cctcttcttt cctgattttg
     5461 aggagtgaac tgatttctgt ctcgttttca aatgctttct gcggattgaa aacgatttta
     5521 tcgcctttgg atttgccggt aaagagattt ttctgttctt catctttcat gtatgccgga
     5581 gtgagcacag catcggccac agtgattccg cctgctttca cttcaccatt ttcaagttcc
     5641 gccaggtttc cttttaccat gtctttttca tctaccgaat ccacctgttc gtattttccg
     5701 aagcgtgagg catatgattt tatctgattg tcaatcattt cggcggatac ggatatttca
     5761 taataaggaa catcgggcat gtcgttcaga tttacgtcga actgaggagc tattccaata
     5821 tcaaaatgaa aatcaaagtc atcctgcgta tcaaagtcaa attctgattt ttcgttcggc
     5881 agcggttgtc ccaaaatctg aatatcgttt tcttcgatga agtttaccag cccttcagac
     5941 accagattat tgatttcttc ggctttcact gcttttccat acattttttt aatgagtccg
     6001 atcggaacgt ttccggggcg gaatcccggc atattggctt ttttacggta gtcacgcagc
     6061 gatttttcaa ccttttcggc ataatcttct ttcgatacac tgatagttat aaccgaatta
     6121 atctgatcaa tgtctttttt tgaaatattc atatcttttt aattattagc acattataag
     6181 ctattattat tctcgggtaa attggggtgc aaagatacaa aattttcaat aaacattcct
     6241 cattttttca ctttttacta ttaaattatt ctggaattaa tttatgcaat atgtattggc
     6301 ctttttgttg atttttctca aattatacat cttaaatatc aaaaatatct ccttttttat
     6361 acaattagtt ttgtaaattc gcacgaaaat aaacatctat acaccccacc atgaacacag
     6421 ccagattttt caaagtcatc ttttttttat tgttgcctgt ttttatgatt tccgcctacg
     6481 gcgaatcacc cgaaatcact tcactcaaag tagaaatgca gacaaatcca aagggtttga
     6541 gcacactttc gccacgtttt tcgtggcaga taaatgcaga caaaccaaat ctgaaacaaa
     6601 cctcctatcg gatacaggtt tcatcctccg aaaaagatct gttgaaggaa aagaatcttc
     6661 tttgggattc ggggcagatt gaaagtgaag tttcgcaact cattccatac agcggattaa
     6721 agcccgggtc ggggacccaa tacttctggc gcgtgagagt gggcacaaat cagggaaaaa
     6781 gcaagtggag caaagccggc agctggtcaa cggcattgtt ggctgactcc gactggaaag
     6841 cccgctggat aggtgaagac acactatcca acccgggaga aacaagttcc aaaaacaccc
     6901 gactggctgc ccgctacctt cgaaaaacct tcacggcaga taaaaaggtg aaacgggctg
     6961 tattgtacat atcaggcttg ggcatgtacg aagcatacat caacggcctg agaatatccg
     7021 aagatgtatt ttcacccact ccatcgtggt atccggagcg tgtttactac aatacttatg
     7081 atgtaactca cctgcttaaa aacggagtga atgtgctggc tgtaaagctt ggcaacggcc
     7141 ggtttttcgg aatgcgcgaa aacaaaacga tgatgttcgg acttccccgc ttgctggctc
     7201 agctcgaaat ggaaaacaca aaccatcaga aaaccaccat aataaccgat caatcctgga
     7261 aaatcacctc cagaggccca atcgtatcta acaacgaatt tgacggcgaa gagtacgatg
     7321 cgcgtttaga aatgaaagga tggaactcac cggagttcga tgatgctggt tgggaaacag
     7381 cagaaataat gaaaaatccg ggcggaaaac ttactgctca gcccaacccg aatattgctg
     7441 tacaggatca tatcaccccg ttgagaatta tcaaaacaac tggaaacaaa tatgtactgg
     7501 atatggggca aaatatggtt gggtggttga aagtaaatct gaaaggcaaa aaggataaac
     7561 ccctgaaaat gagatttgcc gaaattctga aaaaagacag caccctttat ctggataata
     7621 tccggagtgc aaaggtaacc gatatataca ctcccgctac ggatggtatg tttacctggg
     7681 agccgtcatt tacatatcac gggttccgct ttgtggaaat tgagggactt gattatgagc
     7741 cgtcagttac cgatttcgaa ggaaaagtaa tttatgacag gatgacaaca tccggaagtt
     7801 tcgaaacatc cagcgaaatg attaacacga tttacaaaaa tgcattttgg ggtatccggg
     7861 gcaattaccg cggcatgccc accgactgcc cgcagcggga cgagcggatg ggatggttgg
     7921 gcgacagagc taccggatgc tttggcgagg ctttcgtttt tgacaattca cagctttaca
     7981 gtaaatggat tcaagatata gaagattcac agagccccga aggaagcatt tctgtggttt
     8041 cgccccgcta ctggactatc tacaatgacg acgtaacctg gcctgcagcc tatttctatg
     8101 ccgctcagat gctcatcaga caatttggcg acaccaaacc gatcattaag cattatgact
     8161 ccatgaaaaa atacctggaa caaattcagc gaatatccat gaaggattac atcattacca
     8221 aagacacgta tggcgactgg tgtatgcctc ccgaaaagca ggaactcatt cattccaaag
     8281 atccggcccg aaaaaccgat ggagccattc tgagcacaac catgtattac cagctgctga
     8341 atatgatgtg tgaatttgct gaaatttcat caaacaatca ggacatcgaa agctacagaa
     8401 atctcgcttc gaaaattaaa caggcataca acgaaaaata ctttgataaa aacaccgctc
     8461 aatacggcaa caacactgtg acagccaata tactatcact ccgtctgggt ctggttccgg
     8521 ccggatacga gcagcgtgta ttcgaaaaca tagtcaaaaa aacagaaacc gaattcaacg
     8581 ggcatgtcag tacaggagta ttgggcattc aacatctgat gcgcggactt actgaattcg
     8641 gaaagtcaga tttagcattt aaaatagcca ccaacgatac ttacccaagc tggggctaca
     8701 tggcacaaaa aggtgccacc actatctggg aactgtggaa cggtgatact gccgatcctt
     8761 caatgaactc gggaaaccac gtaatgctgc ttggcgactt actgatatgg tattacgaag
     8821 atttggccgg cataaaaaat gctcctgaaa gtaatggtta taaaaaactg ctcatgcaac
     8881 cggtatttcc aaataatctg catcatgtaa aagcttccta ccaatctgta tatggtgaaa
     8941 taaagagcga atggacccgg aaagaaggac ttttcgactg gaaaattacg attccgggaa
     9001 acgcatcggc tatcgtacga ataccgaaag aaattacact caatcaatta aatacaaatg
     9061 aaataataag ccagttaaaa catgcggact ataccgaaat agaggtaggt tcgggccatt
     9121 attcttttac aggaaactat taaccaattc ttaaagattt ataaccgaaa ttagatactt
     9181 caataccaat attttttact aattttgcac ttcatcaaaa aaaatgtatc acaatgaaaa
     9241 atattctgac atttctgtca tttataatta ttacttcatt ctcagtacac tcacaaggta
     9301 tcagtttcga agaaaatcac gacctgaacg cagctttggc taaagcaaaa acagagaaca
     9361 aacttatttt catcgatgct tacgccgtgt ggtgtggtcc ctgcaaagtg atggcacgcg
     9421 atattttccc actcaaagaa gtgggtgatt tcttcaatgc caactttgta aacctcaaac
     9481 tggacatgga agcaaaggaa aatatcaata ttgccaaaaa atacgaggta caggcctatc
     9541 caacttacct ttttctgaat gccgacggtg agttggttca caaaggactt ggaagcatgc
     9601 ctgcagccca atttattgaa gtggccaagg tggctgcaga cacagaaaac aactatgccg
     9661 ctctttccaa aaaaatcaaa aacggcgacc gcagcctcgc aaccgtcaaa aaattcatca
     9721 cacaaaatcc gtacgacacc ggaaacggag cactcgtgga cagctacttc cagacacttt
     9781 ctgaaaatca gat
//
LOCUS       DAORKP010000087         9703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_927_length_9703_cov_9.942890, whole genome shotgun sequence.
ACCESSION   DAORKP010000087 DAORKP010000000
VERSION     DAORKP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_9703_cov_9.942890"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..533)
                     /locus_tag="PLN75_06110"
     CDS             complement(63..533)
                     /locus_tag="PLN75_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRG03723.1"
                     /translation="MDEHEKFMRKAISLSIQNIERGGGPFGAVIVKDGRIVATGVNRV
                     TAVNDPTAHAEISAIRKAAKKLGTFSLEGCTLYTSCEPCPMCLGAVYWAHFDRLFYAN
                     TKSDAKKIGFDDSFIYDEIELNPSERSIKTVRLLPEEAIRAFEFWNEKDDKIEY"
     gene            729..1679
                     /locus_tag="PLN75_06115"
     CDS             729..1679
                     /locus_tag="PLN75_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRG03724.1"
                     /translation="MIEDKIRAGRNQTLTLNEEERVQLFDNLFIPEKNETLVPEKILN
                     KTILSDLFDVLDDLPESFADLIIIDPPYNLSRDFHGFKFKATDEESYADYLNSWFPKI
                     VSILKPNGSLYLCGDWKNTALLQQVMSGHLTVLNRITWQREKGRGASKNWKNGMEDIW
                     FGVKNPDDYYFDVEAVKMKRQVIAPYKKDGKPKDWHETNEGNFRLTHPSNFWDDISIP
                     FWSMPENTDHPTQKPEKLIAKLILASCPPGGTVFDPFLGSGTTSVVAKKLNRNYLGVE
                     MNREYALWAEKRLLLCEQFPQIQGYFDNVFWERNTLKFRK"
     gene            1759..2715
                     /locus_tag="PLN75_06120"
     CDS             1759..2715
                     /locus_tag="PLN75_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRG03725.1"
                     /translation="MILDIVFIVAGFVFLIKGADWLVGGASGLAKKHNVSDLVIGLTI
                     VAFGTSAPELVVNSIASVDKHSDIVFGNIIGSNNFNLFFILGTVALIFPVTVQRSMIR
                     KEIPFSLVITVLVLVLSNAVLGQKENVLSRFEGMALLVLFAAFIYYTFRQSAVDNDVE
                     KIEMKQMTTLKIWALIVIGLSLLVAGGRMVVTGAENIALDLGISEKIIALTIVAAGTS
                     LPELVTSVVAASKKNSDIAIGNVIGSNIFNLLFILPVSAVIHPVSYATTFNREMLLLI
                     GGTIIVILYMFAGKRYKVDRWEGALLLGVFLAYTIFLIQQEL"
     gene            2759..3244
                     /locus_tag="PLN75_06125"
     CDS             2759..3244
                     /locus_tag="PLN75_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03726.1"
                     /translation="MKISRYIFMLVLAVFFGCENKYKSPVPDVPVEISLNMLTYNPTF
                     GSVLNDTMIFVRPRTYPPFAIGYAGVLVVVGYDENLSNRFFSYDLCCPYEADPNVRVY
                     TGMHLLNPEKYQEGYAVCPKCGSEFYITDGWGRVSKGPSKHPLKRYQTDYINNYLIVR
                     N"
     gene            complement(3248..4339)
                     /locus_tag="PLN75_06130"
     CDS             complement(3248..4339)
                     /locus_tag="PLN75_06130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03727.1"
                     /translation="MTLVLISLFILFAYAVMIISFIKGWAITPDFKAEKTGNSCLVAI
                     VTACKNEIENLPLLFSSLKKQTYRNFQFMLVDDDSTDGSLEFASKCTPEFSELTLLKN
                     KGKGKKEAIKTAIVQTDARIIVSLDADCLPSPDWLATIVEFYSQQLTDLIICPVKMTT
                     NGSLFGNFQQFEFAALVASGAGAAGIGIPVLCNGANLAFRRQAWLSSEDRLHFEEPGG
                     DDIFLLQSIKKRGGPIRFLKSGEAAVETAPTKTLKEFFRQRRRWAGKKAAYADWQLAA
                     VALIVFLASFIILVNLAFAFIGLKWLVLAGWLYLMKWMIDFSFFMRIKDFFGLKNVAG
                     NSLLFSLFYPFYIVVTAFFSLFGKRKGKW"
     gene            complement(4375..5244)
                     /gene="murI"
                     /locus_tag="PLN75_06135"
     CDS             complement(4375..5244)
                     /gene="murI"
                     /locus_tag="PLN75_06135"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRG03728.1"
                     /translation="MASSPILSLGSPWSKEAAPIGIFDSGYGGLTILNEIRKELPLYD
                     FLYLGDNARTPYGTRSFDVVYKFTLQCVEYLFGRGCHLIILACNTASAKALRTIQQND
                     LPKIDSSRRVLGVIRPTVEEVGKLTATNHVGLFATSGTVQSNSYPLEIHKIYPDVRVV
                     SEACPMWVPLIENNEFQSPGADYFVRKHTHNLMKQDDRIDTVILGCTHYPLLKEKIEK
                     ELPAHVSVISQGEIVARSLAEYLHRHPEMDKKCTKNGTCSYLTTENPGKFSSSASVFL
                     SEEITARHVEVEI"
     gene            complement(5265..6233)
                     /locus_tag="PLN75_06140"
     CDS             complement(5265..6233)
                     /locus_tag="PLN75_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRG03729.1"
                     /translation="MAKKKFYFNPETVDYEEIQITFSLRLRQILLHTLSGILLGVAFF
                     FLFVSVVKSPKEKELTMQKNKMEGQYKALEGQMKEFQGILADLQERDDNLYRVVFQAD
                     PIPYQSRHAASGNMDYYNELMKMTNSQIVVRTTQKLNELKKQLYVQSKSYDEIVKLAQ
                     SREDLLKCIPAIQPVANKDLKRVASGWGYRYDPIYHTRRFHEGMDFTAPTGTDIFTTG
                     NGTISEAGWQQGYGNCVKVDHGFGYESFYAHMSEIKVSVGQSVTRGEIIGLVGSTGKS
                     TGPHLHYEVHYKGQVMNPQNYYFLDLTPEEYDRMIQLSQNAGQTFD"
     gene            6439..9042
                     /gene="alaS"
                     /locus_tag="PLN75_06145"
     CDS             6439..9042
                     /gene="alaS"
                     /locus_tag="PLN75_06145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRG03730.1"
                     /translation="MTSQEIRQSFLDFFASKDHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPVKYPRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAITWAWEYLTEVLKLDKDRLYVTVFEGSEYEKLERDNEAAEIWSQFLPADRIINGN
                     KKDNFWEMGDTGPCGPCSEIHIDIRPNDERAKVNGLSLVNANHPQVIEIWNNVFMQYN
                     RKADGSLEELPAKVIDTGMGFERLCMAIQGKQSNYDTDIFQPIIKEIGAITNHRYGEN
                     EKTDVAMRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRAVRYGYTFLGQRHAFM
                     YRLIQVLGQVMGSAYPELIAQKTLIEKVIKEEEESFLRTLETGIKLLEKSIPQTSSKG
                     GGMLDGTVAFTLYDTFGFPFDLTELILREHNMTVDVNGFNAEMQKQKERARNAAAVET
                     GDWITVREGDSQFVGYDMTECEAEILRYRKVTQKNKSFYQLVLTQTPFYAEMGGQVGD
                     SGTLTADDEKIEIFDTKKENNLSVHLSNTLPKDVTATFKVQIEIENRLATARNHSATH
                     LLHEALREVLGNHVEQKGSYVSPESLRFDFSHFQKMTREEIREVEKIANRRIRANYPL
                     HEMRETPIAEAQAMGAMALFGEKYGDTVRVIQFGNSVELCGGTHVHATGEIGMVKIVS
                     ENSIAAGIRRIEAITGKAVEDLINNVQDTLFSAKELFSNTPDLTKAIQKTIEENSDLK
                     KQVENFMHERIFALRDKLIAEAKEENDVKTIRFRTELPTDAIKTLAFQIKNVVGNQLF
                     VAIGSIFDGKPSLTVLLSDDLVAQGHSAVNITREAAKFIQGGGGGQPHFATAGGKNAD
                     GLDKALEAIKL"
     gene            9258..>9703
                     /locus_tag="PLN75_06150"
     CDS             9258..>9703
                     /locus_tag="PLN75_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRG03731.1"
                     /translation="MKIQVLKSVFLIVFLVGNFSINAQDWANLKRFQKENDTLLLKPE
                     IKNRVVFMGNSITEGWKGTHPDFFSKNPYVCRGISGQTTPQMLVRFRQDVISLKPEVV
                     VILAGTNDIAGNTGPSTPEMILDNLKSMTELSTANNIRVVLCSVLPA"
BASE COUNT         2861 a         2133 c         2132 g         2577 t
ORIGIN      
        1 tatgaaaata aacggctgaa acacaggagg atgtttcagc cgtttatttt ttttggttgc
       61 tgtcaatact caatcttatc atccttttcg ttccagaatt caaatgcccg gatagcctct
      121 tcgggaagca accgaacggt tttgatactc cgttcagaag gattcagttc tatctcatca
      181 tagataaacg aatcgtcaaa acctattttt ttggcatcgg acttggtgtt ggcgtagaaa
      241 agacggtcga aatgtgccca atacacagca cccagacaca tcggacaggg ttcgcacgaa
      301 gtgtaaagcg tgcaaccttc gaggctgaaa gtgccgagtt ttttggccgc tttgcgaatg
      361 gcactaattt cggcatgtgc cgtggggtcg ttcactgctg tcacccgatt aactccggtt
      421 gcaacgatcc ggccgtcttt gacgattacc gcaccgaaag gtccgcctcc cctttcaata
      481 ttttgaatcg acagcgaaat ggcttttcgc ataaattttt cgtgttcatc catagttgag
      541 cattttttta aaaaataact ttacaaatat agcgtttatg ttgaaaacaa acaatcctga
      601 taactgaata ttaagcccgt aatcgggtga tattgtaatt aatctccaac taacctgcaa
      661 aagcattcga tttttttttc gaactttgca tatcgtatca aaactcactg attagcaaag
      721 ataaaataat gatagaagat aaaatcagag caggcagaaa tcaaactctg acactcaacg
      781 aagaagaacg ggtacaactt tttgataatc tttttatacc cgaaaagaac gaaacgttgg
      841 tacctgagaa aattctgaac aaaacaattc tgtccgattt atttgatgta ttggatgatc
      901 tgcccgaaag ttttgccgat ctgatcatta ttgatccgcc ttataatttg agtcgcgatt
      961 ttcatggatt caaattcaaa gccaccgacg aagaaagtta tgctgattac cttaactcct
     1021 ggtttccgaa aattgtgagt atactgaaac ccaacggatc gctctatctg tgtggcgact
     1081 ggaaaaacac agctttacta cagcaggtga tgagtgggca cctgacggta ttgaaccgca
     1141 tcacctggca gcgcgaaaaa gggcggggtg catccaaaaa ctggaaaaac ggaatggaag
     1201 acatctggtt tggtgtgaaa aatccggatg actactattt cgatgtggaa gccgtgaaaa
     1261 tgaaacgtca ggtaattgca ccctataaaa aagacggtaa accgaaagac tggcatgaaa
     1321 ccaacgaagg caacttcaga ctcacccacc cttccaattt ctgggacgat atcagcatcc
     1381 cgttctggtc catgcccgaa aacaccgatc accccaccca aaaacccgaa aaactgatag
     1441 ccaaactgat acttgccagc tgtccgccgg gtggtacggt gtttgatcct tttctgggct
     1501 ccggtacgac ctcggtggta gccaaaaaac tgaacagaaa ctacctggga gtagaaatga
     1561 atcgggaata tgcgctctgg gccgaaaaaa gattgctgct gtgtgaacaa tttccacaaa
     1621 ttcagggtta ttttgacaat gttttctggg aaaggaatac gttgaaattc agaaagtgaa
     1681 aagggttact taagattgat tttgcactga actcagactc ccggactctt caattgaata
     1741 acataaataa cattttttat gattttagat atcgttttta ttgttgcagg ttttgtgttt
     1801 ttaatcaagg gtgccgactg gctcgttggc ggagcttccg gactggccaa aaaacacaac
     1861 gtttccgatc tggtcatcgg actaaccatt gttgcgtttg gcacatcggc tcccgaactg
     1921 gttgtaaatt cgattgcatc tgtcgacaaa cactcagata ttgtattcgg aaacattatc
     1981 gggagcaaca attttaattt attctttatt ctgggcactg tagcacttat atttccggtc
     2041 acggttcagc gcagcatgat acgcaaagaa atcccgtttt cgctggtaat cacagttctt
     2101 gtgctggtat tatcaaacgc tgttctcggc cagaaagaaa atgtactatc acgtttcgaa
     2161 ggaatggcac ttctggtact ttttgccgca tttatatact acacattcag acaatcggct
     2221 gttgataacg acgtggaaaa gattgaaatg aaacagatga cgaccttaaa aatctgggct
     2281 ctcatagtca ttggattgag tctgttagtt gcaggtggca ggatggtggt gacgggagcc
     2341 gagaatatag cactggattt gggtatttcg gaaaaaataa ttgcgctaac cattgtggct
     2401 gcaggaacct cactgcctga gctggtcact tcggtagtgg cagcatcaaa aaagaacagc
     2461 gatatagcca ttggcaacgt tatcggctcc aacattttca acttgctgtt tattcttccg
     2521 gtgagtgccg tgattcatcc ggtaagctac gctaccactt tcaaccgcga aatgctactg
     2581 cttatcggcg gaacgattat tgtaattttg tatatgtttg caggcaaaag atacaaagtg
     2641 gacaggtggg aaggtgcttt gctgctgggt gtgtttcttg cctataccat tttcctcatt
     2701 cagcaggaat tgtgaaataa aagcggatag ttaccgtttt tatatcaaaa tcattcgctt
     2761 gaaaatttcc aggtacatat tcatgcttgt tttagccgtt tttttcggct gcgaaaacaa
     2821 atacaaatcg cccgtgcccg atgtaccggt agagataagc ctcaacatgc ttacctacaa
     2881 tcctactttt ggcagcgtac tgaacgacac gatgattttt gtacgtccgc gtacctatcc
     2941 tccttttgca atcggctatg caggcgtatt ggtggtagtg gggtatgatg aaaatctgag
     3001 taaccgtttt ttttcgtatg acttgtgctg tccgtacgaa gccgatccga atgtgcgtgt
     3061 gtacaccggc atgcacctgc ttaatcccga aaagtatcag gaaggatatg ccgtttgccc
     3121 taaatgcggt tcggaattct acatcaccga cggctggggc agggtgtcga aagggccatc
     3181 caaacatccg ctcaaacgct atcagacaga ctacatcaac aactacctga tagtgagaaa
     3241 ttaactgcta ccactttcct tttctttttc cgaaaagcga aaaaaatgcg gtaacaacaa
     3301 tgtagaaggg ataaaataac gaaaacagca ggctgtttcc ggccacattt ttcaatccga
     3361 aaaaatcttt tatcctcatg aagaatgaga agtcaatcat ccatttcatc agatacagcc
     3421 agccggccaa tacaagccat ttcagaccaa taaaagcaaa tgccagattc accaggataa
     3481 tgaaacttgc cagaaaaacg atcagcgcga cagcagccag ctgccagtcg gcataagctg
     3541 cctttttacc ggcccatctg cggcgctggc ggaaaaattc tttcaaagtc tttgtgggag
     3601 cggtttctac tgctgcctct cccgatttga gaaaccggat aggccctccc cgttttttaa
     3661 tactctgaag cagaaaaata tcgtcgcctc ccggttcttc gaaatgaagc ctgtcctcgc
     3721 tgctgagcca ggcttgtctg cggaatgcca ggttggcacc attgcacaaa acgggtattc
     3781 cgattccggc tgcacctgct ccgcttgcta ccagggcagc aaactcaaac tgctgaaaat
     3841 tgccaaaaag actcccgttt gtggtcattt tcacaggaca aataatcaga tcggtcaatt
     3901 gctgactgta aaattccaca atggtagcca gccaatcggg tgatggtaag caatcggcat
     3961 ccagcgaaac gataatgcgg gcatccgttt gtactatagc ggttttgata gcttctttct
     4021 tacctttgcc tttgttttta agtagtgtca gttccgaaaa ttccggagta cacttgctgg
     4081 caaattccag gctgccgtcg gttgaatcgt cgtcgacgag catgaactga aaattgcggt
     4141 aagtttgttt tttcagcgaa ctgaagagca ggggaagatt ttctatttcg tttttacaag
     4201 ccgtaactat tgccaccaga caactgttcc cggttttttc ggccttaaaa tcgggcgtta
     4261 tcgcccatcc tttgataaaa ctgattatca ttacggcata ggcaaaaaga atgaaaagtg
     4321 aaatcagaac aagtgtcatt tccgttctct ttttttacaa aatggggcaa aaaatcagat
     4381 ttccacttcc acgtgtcttg cggtgatttc ttccgacaaa aagacagaag ccgatgatga
     4441 aaattttccc ggattttcgg tggtcagata actgcaagtg ccgttttttg tacatttttt
     4501 atccatttcg gggtgacggt ggaggtattc ggccagactc ctggctacaa tttcaccctg
     4561 cgaaatcacg ctgacatgag caggcagttc tttttcaatt ttttctttca gcaacggata
     4621 gtgtgtgcaa cccagaatta cagtgtcaat cctgtcatcc tgcttcatca gattgtgagt
     4681 gtgttttctg acaaaataat cagctccggg gctttgaaat tcattatttt cgatcagcgg
     4741 cacccacatc ggacaggctt ccgaaacaac ccgcacatcg ggataaattt tatgaatttc
     4801 gagcggatat gagttggact gaacagtgcc gctggtggca aataatccca catgattcgt
     4861 tgctgtgagt ttccctacct cttccaccgt tggccgaata acacctaaaa ctctgcggga
     4921 agaatcaatt tttggcaaat cattttgctg tatggtgcgc aaggctttcg ccgaggcagt
     4981 attgcaggcc agaataatca aatggcagcc ccgaccgaac aaatattcca cacactgaag
     5041 ggtaaacttg taaacaacat cgaatgagcg ggttccgtac ggagtgcggg cattatcgcc
     5101 cagatacaaa aaatcgtaaa ggggcaattc cttccgaatc tcattcagga tggtaagtcc
     5161 gccgtagccg gaatcaaaaa taccgatggg agctgcttcc ttactccagg gtgaaccgag
     5221 cgaaagaatc ggagaagatg ccatagttat gtgttataaa tgaattaatc aaaagtctga
     5281 cctgcattct ggctcagctg aatcatccgg tcgtattctt cgggcgtaag atccagaaaa
     5341 taataattct gcggattcat cacctgacct ttgtaatgaa cttcgtaatg aagatgcggc
     5401 ccggtggatt ttccggtgct gccgacaagc ccgatgattt cgccccgcgt aaccgattga
     5461 cctacagata cttttatttc ggacatgtgt gcgtagaaac tttcataacc gaatccgtga
     5521 tctactttta cacagttgcc gtaaccctgt tgccatccgg cttctgatat tgttccgttg
     5581 cctgttgtaa aaatatcggt accggtggga gccgtaaagt ccatcccttc atgaaaacgc
     5641 ctggtatgat aaatcggatc gtaacgataa ccccagcccg aagccacccg tttcaggtct
     5701 ttattggcca caggctgtat ggcaggaatg catttgagta aatcttcgcg actttgagcc
     5761 agctttacaa tttcgtcgta tgatttcgac tgaacgtaca gttgcttctt taattcattc
     5821 agtttttgag ttgtcctcac cacaatctgt gaattggtca ttttcatcag ctcattgtaa
     5881 taatccatat tgcccgaagc ggcatgcctg gactgatacg gaatgggatc tgcctgaaaa
     5941 accacacgat acaggttgtc atcccgctcc tgcaagtcgg caagtatgcc ctgaaactct
     6001 ttcatttgcc cctccaatgc tttgtactga ccttccatct tgtttttctg catagtcagt
     6061 tccttttctt tgggagattt gaccaccgac acaaacagga aaaagaaagc aactcccagc
     6121 agaatacctg aaagcgtatg tagcagaatt tgtctcaaac gaagggaaaa tgttatctga
     6181 atctcctcgt aatcaacagt ttccggatta aaataaaact ttttcttcgc catacacgta
     6241 ttttggatag ataaagacat atcgagagca gattaggaag gtaaattact ctccgactgc
     6301 tgttttttta ccagaatgtt gatgcaaaag taataatttt gaagtacttt tgcagattaa
     6361 attgaaaaaa tattgtatta tggcagatat aactgaaacc tgccggctgc gtacaccatt
     6421 taacaaaatt attttacaat gacttcacaa gaaatcagac aatcattttt agattttttt
     6481 gcttctaaag accacaaaat cgttccctct gctccgatgg taatcaaaga tgaccccacg
     6541 ctgatgttta ccaatgcggg aatgaaccaa ttcaaaaata taattctggg aaacgatcct
     6601 gtaaaatatc cccgcgtagc cgactcgcag aaatgtctgc gcgttagtgg caagcacaac
     6661 gacctggaag aggtgggtca cgacacctac caccacacca tgttcgaaat gctgggcaac
     6721 tggtcattcg gcgattattt caaaaaagaa gccattacct gggcgtggga atacctcacc
     6781 gaagtgctca agctggacaa agaccgattg tatgtcacgg tattcgaagg ctctgaatac
     6841 gaaaagctgg aacgtgataa cgaggctgcc gaaatttggt cgcagtttct gccggccgac
     6901 cggattatca acggaaataa aaaagataat ttctgggaaa tgggcgacac aggaccctgc
     6961 ggaccctgtt cggaaattca tatcgatatc agaccgaacg atgaacgtgc aaaagtaaat
     7021 ggattgtcat tggtaaatgc aaaccatcca caggtgattg aaatctggaa caatgtattc
     7081 atgcagtaca accgcaaagc cgatggttcg cttgaagaac ttccggccaa ggtaattgat
     7141 acgggcatgg gttttgaacg gctttgtatg gctatacagg gaaaacaatc caattacgat
     7201 accgatattt tccaaccgat aatcaaagaa atcggagcaa tcacaaacca ccgatacggt
     7261 gaaaacgaaa aaacagacgt ggccatgcgc gttattgcgg accacatccg taccattgcc
     7321 ttttccatta ctgatggcca gttaccttcc aatgccaaag cgggttatgt aatacgccgg
     7381 attctgcgcc gcgccgtacg ctacggatac acgttcctgg gtcagcgtca tgcatttatg
     7441 taccggctta ttcaggtgct tgggcaggta atgggaagtg cctacccgga attgattgct
     7501 cagaaaacgc tgatcgaaaa agtgataaaa gaagaggaag aatcgtttct gcgtacactg
     7561 gagacaggaa tcaagttgct ggaaaaaagc attccgcaaa cctcctccaa aggaggagga
     7621 atgttagacg gtacagtagc ctttacgctt tatgacactt ttggttttcc atttgacctg
     7681 acggaattga tactccgtga acataatatg actgttgatg tgaatggttt caacgctgaa
     7741 atgcaaaaac aaaaagaacg ggcacgtaat gctgctgccg ttgaaaccgg cgactggatt
     7801 accgttcgcg agggcgacag ccagtttgtg ggatacgata tgacagagtg cgaagccgaa
     7861 atactccgat accgcaaagt gacacaaaaa aacaaatcat tctaccaact tgtacttact
     7921 caaactccat tctacgccga aatgggagga caagtgggtg actccggcac gttgactgcc
     7981 gatgatgaaa aaatagaaat ttttgataca aaaaaagaaa ataatctttc ggttcatctt
     8041 tcaaacacac tcccgaaaga tgtaacagct actttcaaag tacaaatcga aattgaaaac
     8101 cgcctggcta ctgcccgcaa tcactccgcc actcacttac tccacgaagc attgcgcgaa
     8161 gtacttggaa atcatgtgga gcaaaaaggt tcttatgtta gccctgaaag ccttcggttc
     8221 gacttttcgc attttcagaa aatgacccgt gaagaaatac gtgaagtgga gaaaattgcc
     8281 aaccggagaa tacgggccaa ttacccactt catgaaatgc gcgaaactcc gattgccgaa
     8341 gctcaggcga tgggtgctat ggctcttttc ggcgaaaaat acggcgatac cgttcgtgtc
     8401 attcagtttg gcaactcagt agaactttgc ggaggcacac atgtgcacgc tacaggcgaa
     8461 ataggcatgg tgaaaatagt aagcgagaat tcaatagcag ccggaatacg ccgaatagag
     8521 gctatcaccg gcaaagcggt ggaagacctg attaacaacg tacaagacac tttattctca
     8581 gctaaagaac tttttagtaa tacgcccgat ctgacaaaag ccattcaaaa aacaatagaa
     8641 gaaaacagcg acctgaaaaa acaggtggaa aacttcatgc acgaacgtat ttttgcgttg
     8701 cgtgacaagc tgatagctga agcaaaggaa gaaaacgacg taaaaacaat ccggttccgc
     8761 accgaactgc caacagatgc catcaaaaca ctggcattcc agattaagaa tgtagttggg
     8821 aatcaactat ttgtggcaat cggcagcatt ttcgacggta aaccttccct gactgttctt
     8881 ctgtctgacg acctggtggc acaaggacac agtgcagtta acattacccg agaagctgcc
     8941 aaatttattc agggtggcgg aggcggacaa cctcactttg caacagcagg cggcaaaaat
     9001 gccgatggac tggataaagc actggaagcg ataaaactgt aaagatgccc caacctcccg
     9061 aagcaagtgc gggacaggct ctaaaaggga agttttgacg gattgtaaca tactggattt
     9121 tagcggaaaa tgaatataca actaacttaa tcaagattaa aaaaattgtg gagcaaattg
     9181 tggtttaacc catagtgtat tttgataata aattatttgc tttaacagaa tattaaaata
     9241 aacagataga taagactatg aaaatccagg tattaaaatc agtttttcta atcgtatttc
     9301 tagttggtaa tttcagtatt aatgcgcagg attgggctaa tctgaaacga tttcaaaaag
     9361 agaatgatac gcttctatta aaacctgaaa tcaaaaacag ggtggtattt atgggaaatt
     9421 ccatcactga aggctggaaa ggtacgcatc ccgacttttt cagcaagaat ccctatgttt
     9481 gccgtggcat aagcggacaa actaccccgc aaatgctggt acgttttcgt caggatgtga
     9541 ttagcctgaa gcctgaagta gtcgtgattc tggccggcac taatgacatt gccggaaata
     9601 cgggaccctc cacaccggaa atgattctgg acaatctgaa atccatgacc gaactctcta
     9661 ctgccaataa tattcgggta gtgctttgct cggtacttcc ggc
//
LOCUS       DAORKP010000088         9658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_937_length_9658_cov_8.221806, whole genome shotgun sequence.
ACCESSION   DAORKP010000088 DAORKP010000000
VERSION     DAORKP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_9658_cov_8.221806"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLN75_06155"
     CDS             <1..522
                     /locus_tag="PLN75_06155"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRG03732.1"
                     /translation="GGSSDAAFMLKALNELFELKIPTRKLEQLAAGLGADCPFFIRNE
                     PVYATGIGNVFTPIDLSLRDYWLLLVKPDIHVSTAEAYAGITPAQSEISLTELIKLPV
                     ETWKNCIKNDFEKSVFEKQPEIGKLKNQIYDLGALYASMSGSGSSVYGIFDEKPDFTG
                     LFDGFFVACGLMD"
     gene            complement(597..2066)
                     /locus_tag="PLN75_06160"
     CDS             complement(597..2066)
                     /locus_tag="PLN75_06160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRG03733.1"
                     /translation="MNSSGFLSAQSFSNDLGVFCYHKDIGEVKIKGDAMYNSEKQEYT
                     LTGAGKNIWFGSDEFHYLYKKIKGDFILTAQVHFTGKGTDPHRKTGWMIRQSLDSNSP
                     HISALLHGDGLTSLQYRKAAGADMDEKKLEITAPDVIQLIRKGNKYTMSAAQWGDTLI
                     TEQIMGDIMSDEVFIGIFICSHNPDVTEKAVFENVRIEIPAGDELVPYRDYLGSNLEI
                     MDVATGKRQIIHQVAYSIQAPNWTPDNKTLIYNSKGLLYSFDLASGNTSVINTKFADN
                     NNNDHVLSSDGKFIGISHHSKKDNGQSVIYYLPVNGGKPKRVTSKSPSYLHGWSPDGK
                     FMIYTGGRNDKYDIYKISKKGGKEIQLTDSEGLDDGSEYSPDGKYIYFNSSRSGKMQI
                     WRMKPDGSEQEQVTSDNLNNWFPHLSPDGKWIVFLTFNNDVKPDDHPFYKHVYIRMMP
                     TAGGKPQVIAYLYGGQGTINVPSWSPDSTKIAFVSNTGF"
     gene            complement(2145..3641)
                     /locus_tag="PLN75_06165"
     CDS             complement(2145..3641)
                     /locus_tag="PLN75_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRG03734.1"
                     /translation="MQAQQNDIVVIIRPVTQGYDYVKSADIQATEYMFPERIDAFEID
                     EMDGVAAVQLRGVSKNGKSLNKSGDFVLYDLKTQMPRWSKKINFPEENATLTGGRPFL
                     LKPYSVTALDEATGAEKWTGKTQIFYADKKTGTGVGYKFNSLNPASDNLEGIDLNNGN
                     TIWKRNLDRTYGWNDITKINDSLILITSGGLYAINLKNGLGWDYKAHTGKKQTGEAVA
                     KGLGSVMLGVLTGVTVIPNVDVITGIVSNTLSDSTGIYLASAGQIGRLNSNGTEIWTT
                     QLPKDLCSASAVYRKNGNIYLINKGIAYENGNMREYGRPFVAVFNEMDGAQVYFRSFD
                     EKKLILNDFEVQGDTINLLFHNKIVQLSLKTDTVLSEKSFDVKNSGYLYSFANDKTFF
                     QQDDTFVPARQNRSKYLITTSTGNVLILNDKMETEQKLTTGEFHKKLFESPTCKLLYK
                     EGSLAVVSHDGKRLAEIKTSPKVLLKGSKLYEAVDKVLREIELDDIIP"
     gene            4061..5320
                     /locus_tag="PLN75_06170"
     CDS             4061..5320
                     /locus_tag="PLN75_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRG03735.1"
                     /translation="MVKESTLEKLKILADAAKYDVSCASSGTTRGNPNKTLGTAAGWG
                     ICHSFTADGRCISLFKILMTNHCIYDCAYCSNRRSNDLKRVAFSPAELAELTIEFYRR
                     NYIEGLFLSSGVIRNPDYTMEQMISVVKKLREEHRFNGYIHMKSIPGASQKLVNIAGM
                     YVDRLSVNIEIPTEESLKKLAPEKDYLSVWKPMKYIKQGILENTEDRKKFRKTPRFVP
                     AGQSTQMIVGATPETDNQILTLASKLYNIDSMKRVYYSGYIHINQNDDRLPALKQPPL
                     VREHRLYQADWLLRFYNFQVNEIVNDANPELDLEVDPKVSWALRHPEFFPIDVNTAPY
                     EAILRIPGVGVLSAKKIITARRYGRLTSYQLQKIGVVMKRAKYFIVCRELPVSTVNEV
                     SPETIRKQLIDEKIKEKDRFQLSFTFD"
     gene            5364..6125
                     /locus_tag="PLN75_06175"
     CDS             5364..6125
                     /locus_tag="PLN75_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HRG03736.1"
                     /translation="MTAFTYDNSFEGLLCCVFYAFEQQVTPDILLHSDDVKPLFLEKS
                     FPIVTESEKSARVWSALEKKISPSACHMLMLVWISELPEVDLLLFRYIYKAFKSTESI
                     ELNFGDADVLACANIYKKVNQSCHKAIQFVRFQKTSDGVFFAPISPDYNVLSLVAKHF
                     SNRYADQPWIIYDTKRNYGLYYNLHKTTEITFSESPVDNSGKLKAEQLDSEEQLYQQM
                     WKDYFKTMAIKERINLKLQRQHMPVRYWKYLTEMQ"
     gene            complement(6151..7527)
                     /locus_tag="PLN75_06180"
     CDS             complement(6151..7527)
                     /locus_tag="PLN75_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG03737.1"
                     /translation="MTNAASNPKSLKQKFSNAFWVANGVELLERLAYYAVFIVITLYL
                     SNVWGFSDIEAGAISGVFSASLYLLPLFSGAYADKIGFRSAIILAFSLLSLGYAGLGI
                     LPTLLESAGLVDYSMTTTFNGLNESYLRWTIVPVMALIVLGGSFIKSVISGTVARETT
                     SENRARGYSIFYMMVNIGAFTGKTVVDPLRKSMGDQGLVYLNFFSASMTLLALIAVIF
                     FYKSVKTEGQGKSFREIGQGLIRVLTNWRLLILILIISGFWLIQSQMYATMPKYVIRL
                     IGEDASPGWYANVNPLIVFVMVNFVTSLMKKKKALSSMMIGMLIIPVSALVMSFGNQI
                     DSPSILGLHPVAFMMILGIAMQAIAECFISPRFLEYFSLQSPKGEEGLYLGFSHLHSF
                     FSYLLAFLMGGFLLDKYCPDPRLFSSAEAYAAATEHAHYIWYVFVVVGLISALALFIY
                     GKVTREKV"
     gene            complement(7861..8334)
                     /locus_tag="PLN75_06185"
     CDS             complement(7861..8334)
                     /locus_tag="PLN75_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRG03738.1"
                     /translation="MKLSYLFLSISLALIVSSCGTTKQSAKTKYNAQLAQKLGADELG
                     MKMYVLVILKTGDTKIEDKALRDSLFRGHFANINKLADEGKLVVAGPLEKNENQYRGI
                     FILDVKTFAEAGLLLQNDPTVSQGIFKPEMYEWYGSAALPVYMQMHKKIQKKQVK"
     gene            8560..8946
                     /locus_tag="PLN75_06190"
     CDS             8560..8946
                     /locus_tag="PLN75_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG03739.1"
                     /translation="MAYSYDVKSKRSGINKNDVKYYAIPKRTGVVTTRQLAKIISERC
                     TLTEPDMVAALSALSKVLEEYLVQGYHVKLDNIGRFSLSVTSDGMQTPEQVTASHVKA
                     KKICFMADKELKLKTANVEFKRKSVK"
     gene            9012..>9658
                     /locus_tag="PLN75_06195"
     CDS             9012..>9658
                     /locus_tag="PLN75_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03740.1"
                     /translation="MTVNLIGRGGATTPTIENPCGICYNVSNDGSTTDIGSNREECLK
                     LIQPTYTPSTSLKVFSNNIENGEYYSAIYLEKSSFNTGDTFSVVINGMSATAFALKGE
                     SNSTVVNKLKNAIISVDNGARAEVDDNNYLYVIYPNRYVYQSDLIAKFASVTSSYHVW
                     VPCLDDYTVRVYANNERPSITVNESEPVYITWDSFDTAKSCNCSYINDDGLTKFCG"
BASE COUNT         2910 a         2013 c         1930 g         2805 t
ORIGIN      
        1 ggtggttcgt cggacgctgc cttcatgctg aaagctctga atgaactgtt cgaactgaaa
       61 attccgaccc gaaaactgga acagctcgcc gccggactcg gagctgattg tccgtttttt
      121 atccggaatg aaccagttta tgccacgggt atcggaaatg tatttacacc gattgattta
      181 tcgctcagag attactggct gctgcttgta aagccggata ttcatgtttc cacagctgaa
      241 gcttatgcag gcattacacc tgcacagtct gaaatatcgc tgactgaatt aattaaactt
      301 ccggttgaaa cctggaaaaa ctgcattaaa aacgattttg aaaaatcggt tttcgaaaag
      361 cagcctgaaa tcggcaaact gaaaaaccaa atctatgacc tgggtgcact ctatgcatcc
      421 atgtccggtt ccggctcatc ggtctatggt atttttgatg agaaaccgga cttcacaggt
      481 ttgttcgatg gattttttgt agcctgcggt ttgatggatt gatttttacg gataaaaacg
      541 attctgtttt ttctcttttc aaatcatgat taagaatcga ttattttggc tgcaatttaa
      601 aatccggtgt tgctcacaaa tgctattttg gtgctgtcgg gcgaccagga aggcacattg
      661 attgtaccct gtcctccgta taaataagca attacttgag gttttccacc cgctgtgggc
      721 atcatacgga tgtaaacatg cttgtaaaac ggatgatcat ccggtttaac atcgttattg
      781 aatgtaagga atacgatcca ttttccatcg ggtgacaggt gtggaaacca gttattcaaa
      841 ttgtcagaag tgacttgttc ctgttcgctc ccatcgggct tcattcgcca gatttgcatt
      901 tttccactgc gcgatgagtt gaaatagatg tattttccgt cgggtgaata ctccgaacca
      961 tcatccagcc cttcagaatc tgtaagttgt atttcttttc ctcctttttt tgaaattttg
     1021 tatatatcgt atttgtcatt tcgaccaccg gtgtaaatca tgaatttccc gtcgggcgac
     1081 catccgtgaa gatacgaagg gctttttgag gtgactcttt tcggctttcc tccatttacg
     1141 ggaaggtaat aaatcaccga ttgcccgttg tcttttttac tgtgatgact tattccgatg
     1201 aattttccgt cggaactgag cacgtgatca ttgttattgt tatctgcaaa ttttgtattt
     1261 attaccgagg tgttgccgga tgctaaatca aaggaataaa gcaagccttt actattgtat
     1321 atcagggttt tattatcagg tgtccagttc ggagcctgga tagaatatgc tacctggtgt
     1381 ataatctgcc gcttaccggt tgccacatcc attatttcga gattgctacc cagataatct
     1441 ctgtaaggta ccagctcgtc gccggcagga atttcaattc ttacgttttc gaaaacagct
     1501 ttttcggtta catccggatt gtgggagcaa ataaatatgc caataaacac ttcatcggac
     1561 atgatgtcac ccatgatttg ttcggttata agcgtatctc cccattgagc agctgacata
     1621 gtgtatttat ttccttttct gataagctga attacatcgg gagctgtaat ttccagtttt
     1681 ttttcatcca tatcggcacc tgctgctttt cggtattgaa gggatgtaag tccgtcgcca
     1741 tgaagtaaag cactgatatg aggcgaattt gaatcgagcg actgacgaat catccagcct
     1801 gttttacggt gaggatcggt gcctttgccc gtgaaatgaa cctgagctgt aagaataaaa
     1861 tctcctttta tttttttgta cagataatga aattcatcac tcccaaacca aatgtttttt
     1921 ccggcaccag taagtgtata ttcctgtttt tcagaattat acatcgcatc accttttatt
     1981 ttcacttcac cgatatcctt gtggtaacag aaaacgccta aatcgttgct aaacgactga
     2041 gcagataaaa atccggaaga attcataaca taaattgcta atgataaaac gttaaaccaa
     2101 attctgtaat ttttcataac aattgatttt taaaagattc agtttcaggg tatgatatca
     2161 tccagttcaa tctctctcag cactttatcg acggcctcat agagtttact gcctttcagt
     2221 aaaactttgg gtgaagtttt tatttcagcc agccgtttgc catcgtggct caccacagcc
     2281 agacttcctt ctttgtacag caatttgcat gttggactct cgaatagttt tttatgaaat
     2341 tctccggtgg taagtttctg ttctgtttcc atcttatcgt tcagaatcag cacattaccc
     2401 gtgctcgttg ttatcaggta tttcgatctg ttttggcggg cagggacaaa tgtgtcgtct
     2461 tgctgaaaga aagttttatc gttggcaaag ctgtagaggt atccggagtt tttcacatca
     2521 aatgatttct cagaaaggac agtgtctgtt ttcaacgaga gttgcactat cttgttatga
     2581 aacaggagat taatggtatc tccctgtact tcaaaatcat tcaggatgag ctttttctcg
     2641 tcgaaacttc tgaaatacac ttgtgctcca tccatttcgt taaatacagc tacaaaagga
     2701 cgtccgtatt ctctcatatt tccgttttca taggctatac ctttgttaat caggtaaata
     2761 tttccattct tacgataaac tgctgaagcg ctgcaaaggt ctttcggtaa ttgagtggtc
     2821 cagatttcag taccattaga attcagacgg cctatttgcc ctgcagaagc caggtaaatg
     2881 cctgtactgt ctgagagtgt atttgatact atgccggtga ttacatcaac gtttggaata
     2941 accgtcacgc ccgtgagtac tcccagcatt acactgccca acccttttgc caccgcttcg
     3001 ccggtttgtt tttttcctgt atgagctttg taatcccagc ccaacccgtt tttcagattg
     3061 atggcataca aaccgccgga cgttatcagg attaggctgt cgttgatttt tgttatgtcg
     3121 ttccagccat aggtgcgatc caggttgcgt ttccagattg tatttccgtt gttcaaatca
     3181 atgccttcca aattatctga agcaggattc agggagttaa atttataccc cacccctgtt
     3241 ccggtttttt tatcggcgta aaagatttgt gtctttcctg tccatttttc agcaccggta
     3301 gcttcgtcga gtgctgttac gctgtagggt ttcaacagaa atggtcgtcc gcctgtcaac
     3361 gttgcatttt cttccggaaa attgattttt ttggaccacc gtggcatttg cgtcttcaaa
     3421 tcataaagta caaaatctcc tgatttgttc aggctctttc cgtttttact cactccgcga
     3481 agttgtacag ctgcaactcc atccatttca tcaatctcaa acgcatcaat tctctcggga
     3541 aacatgtatt cagttgcctg aatatcagca ctcttcacat agtcataacc ctgagtgaca
     3601 ggcctgatta tcacaacaat gtcattttgt tgagcttgca tgacggatgc aaagaagaga
     3661 cagatcaaag taaagcggaa tttattcatg ttatttttgt aaaaataaaa attaagatta
     3721 ataaaaatgt tttataaagc tgtataaatt caattcaagt ttatcaaatc aataagcatt
     3781 gaattttatc ttcataacgg tattcagaat gctatttgac acaacaaaat aaatcaaata
     3841 aatccgaaca aacaacttat tccgaaaaaa tttcaataag cctgaatcat tgtgctgaaa
     3901 aggatttcga agtatcaatc agaccggata aaacttaaac tccggataaa aaaaagatac
     3961 ctgtaccgac aaaaaaaagc tcgaaaaaat ttatcaacac ctcaataata tttagaattc
     4021 attgacataa atagtcggaa aattttgctt actttgtaat atggtaaaag aaagtacact
     4081 cgaaaagcta aaaatccttg ctgatgctgc caaatacgat gtttcgtgcg catcaagcgg
     4141 aactacgcgc ggcaatccga acaaaacgct cggaacagct gccggctggg gtatctgcca
     4201 cagttttacg gctgatggca ggtgcatttc gcttttcaaa atcctgatga ccaaccactg
     4261 catatacgat tgtgcgtatt gctccaacag gcgcagcaat gacctgaaac gtgtagcctt
     4321 cagccccgcc gagctggccg aactaaccat tgaattttat cgcagaaatt acattgaagg
     4381 attatttctg agttcggggg tcatacggaa tcccgattac acgatggaac aaatgatttc
     4441 tgtggtgaaa aaactgcgtg aagaacatcg tttcaacggg tacatccaca tgaaaagcat
     4501 tccgggtgcc agccaaaagc ttgtgaacat agccggaatg tacgtggaca ggctgagtgt
     4561 aaacatcgaa atccccacgg aggagagcct gaaaaaactg gcccctgaaa aagactacct
     4621 gagtgtgtgg aaaccaatga aatacatcaa acaaggtatt cttgaaaata ccgaagaccg
     4681 aaagaaattc cgtaaaaccc ccagatttgt tccggccggt caaagcacac agatgattgt
     4741 gggcgccaca cccgaaactg acaatcagat acttacactc gcatcaaagc tgtacaacat
     4801 tgacagtatg aaacgggtgt attactccgg ttatattcac atcaatcaga acgacgacag
     4861 gcttcctgcc ctgaaacaac cgccactggt tcgcgaacac cggctctatc aggcagactg
     4921 gctgcttcgt ttctacaatt ttcaggtaaa tgaaattgta aacgatgcca atcctgaact
     4981 cgatctggaa gtggatccga aagtttcgtg ggcgcttcgt cacccggagt ttttccctat
     5041 tgacgtcaat acagcaccct acgaagctat tttacgaatt ccgggagtag gagttttatc
     5101 ggcaaaaaaa ataattacgg cacgccgata cggcagactg accagctatc agcttcaaaa
     5161 aatcggcgtg gtaatgaagc gtgccaaata tttcatcgtt tgtcgtgagt tgcctgtcag
     5221 caccgtgaat gaagtgagcc cggaaaccat acggaaacaa ctgattgatg aaaaaatcaa
     5281 ggaaaaagat cgttttcagc tgagttttac attcgattga ttgctcaatt cactttcttt
     5341 attcaaccac taaccccgga aaaatgaccg cttttaccta cgataattcg tttgaaggat
     5401 tgctgtgttg tgtgttttat gctttcgaac agcaggttac acccgacatc ctgcttcatt
     5461 ctgatgatgt aaaaccgtta tttctggaga aatcattccc cattgtgacc gaatcggaga
     5521 aatcggcccg tgtatggagt gcgctcgaaa agaaaatttc accttccgct tgtcacatgc
     5581 ttatgctggt ctggatttca gaactgccag aagttgattt gcttcttttc agatacattt
     5641 acaaggcatt caaatcgaca gaatccatcg aactcaactt tggcgatgcc gacgtgcttg
     5701 cctgtgccaa tatttacaaa aaagtaaatc aaagttgcca caaagccatt cagtttgtca
     5761 gatttcagaa aacatccgat ggtgtatttt ttgcacccat ctcgcccgat tacaatgttc
     5821 tgtcactggt ggcaaaacat ttcagcaatc gttatgccga tcagccctgg atcatttatg
     5881 acaccaaacg gaattacgga ttgtattata atttacacaa aacaacagaa atcacatttt
     5941 cagaaagtcc tgtggataat tcaggtaagt tgaaagcgga acagttggac tccgaagagc
     6001 aactttacca gcagatgtgg aaagattact tcaaaacaat ggctataaaa gaacgaatca
     6061 acctgaaact tcagcgccag cacatgccgg taaggtactg gaaatacctt acagaaatgc
     6121 agtaaatttt taaagggata tatcccggta ctatactttt tcccgggtaa cttttccgta
     6181 aataaacaat gcaagcgccg aaataagccc tacaaccaca aacacatacc agatgtagtg
     6241 tgcatgctcg gtggctgcgg cataagcttc ggccgaactg aacaggcgcg gatcggggca
     6301 atatttatcg agcaggaatc cacccatcag aaacgcgagc aaataggaaa aaaaggaatg
     6361 caggtggctg aaaccgagat acaagccctc ctcacctttg ggggactgca gcgagaaata
     6421 ctccagaaaa cggggtgaaa taaaacactc ggcaatggct tgcatggcaa tacccaaaat
     6481 catcataaaa gctaccggat gaagccccag aatagacggc gaatcaatct gatttccgaa
     6541 tgacattacc agtgccgaca ccggtattat gagcatcccg atcatcatgg acgaaagcgc
     6601 ttttttcttt ttcatgagcg aagtcacaaa gttaaccatc acaaacacaa tgagcggatt
     6661 cacattggcg taccagccgg gtgatgcatc ctcgccaatg agacgtatga cgtatttggg
     6721 catggtggcg tacatctgac tctgaataag ccagaaacca ctgattatca gtatcagtat
     6781 taacagcctc cagtttgtca gcacccgaat aagcccctgt ccgatttcgc ggaaactctt
     6841 tccctgtcct tcggttttca ccgatttata aaagaagata acagcaatta gtgccagcaa
     6901 ggtcatagat gctgaaaaga agttcagata caccagcccc tgatcgccca tgctcttacg
     6961 aagcggatca accaccgttt ttccggtaaa agcaccgata tttaccatca tgtaaaaaat
     7021 agaataaccg cgcgcacggt tttcggaagt agtttcgcga gctacggtac cagaaatcac
     7081 cgacttgata aacgaaccgc caagtacaat caaagccatt accggaacaa tagtccaacg
     7141 cagatagctc tcattcaaac cattgaaagt ggtagtcatg gaataatcca ccaatccggc
     7201 actctcaaga agagtaggaa gaatacccag tccggcatat cccagcgata aaagactgaa
     7261 agccaatata atggccgaac ggaaaccaat tttatcggca taagctcccg aaaacaacgg
     7321 aagcagatac aacgaagcag aaaaaacgcc cgaaatggca cctgcctcaa tatccgagaa
     7381 accccagaca ttggagagat aaagtgtaat gacgataaaa acagcgtaat atgccagtcg
     7441 ctcaagcagt tcaactccgt tggctaccca aaaggcattg gagaattttt gtttgagaga
     7501 tttcggattg gatgcggcgt ttgtcatagg tctattttta tttttaaaaa acagatgcaa
     7561 agttacacaa agtgagtggt ttaaaaaatg caaaaatttt atttacggaa ttttaagggc
     7621 tattgggaaa attaactaaa gttgaaaatt cagacttact tttatatttt ttacgttcca
     7681 tttgggtgag ttttcaatca accggattaa ttcagggttc atttccggac aggatatttt
     7741 ttttatttcc atttctgaag gcattccacg ggaatcaatc gccaataaaa cattgatatt
     7801 caaagtcaca gtgtcaaatg aaagcaactt aacatcgaga ttaaagaatg ttattaaaaa
     7861 ctatttcacc tgcttttttt ggattttttt atgcatttgc atatatacgg gcagcgctgc
     7921 agaaccgtac cattcataca tttcaggttt gaaaattcct tgcgaaaccg ttgggtcgtt
     7981 ttgaagcagc aaacctgctt cggcaaatgt ttttacatcc aggataaaaa taccccgata
     8041 ttggttttcg ttcttttcca atggtccggc aacaactaat tttccttcgt cagccagctt
     8101 gttgatgttt gcaaaatgcc cccggaataa actgtcgcgc aaggctttgt cttcaatttt
     8161 agtatccccg gttttcagaa taaccaacac atacattttc atcccgagtt catcggcacc
     8221 cagtttttga gccaactgag cattgtattt tgtttttgcc gactgttttg ttgttccaca
     8281 actgctcaca ataagggcaa gggatatgga taaaaataaa taagataatt tcattttgag
     8341 ttgaatatga cggtttgtaa tttctatgat atactaagaa caatcaaaat atcagaaatg
     8401 ttggaagaca acttttgaaa atatttatat aaatttactg atattctttg caaaagatgt
     8461 agatgtattg tgtaaaaaga tgtacatttg ttatatcaaa acatctacat ctattatgta
     8521 tatagatgta catctgttaa tcaaaacttc aggaagatta tggcatattc ttatgatgtg
     8581 aaaagcaaaa gaagcggtat aaataaaaac gatgtaaagt attacgctat accaaaacgt
     8641 acaggcgtgg tcacaacccg ccagctggca aaaatcattt cagaacgttg caccctcacc
     8701 gagcccgata tggtggcagc actttcggca ttatcaaagg tgttggagga atatttggtg
     8761 cagggctacc acgtaaagct ggacaatatc gggaggtttt cgctttctgt taccagtgat
     8821 ggtatgcaaa cccccgaaca agtaaccgca tcgcacgtaa aagcaaagaa aatctgcttt
     8881 atggccgaca aagaactcaa actgaaaaca gcaaacgtgg agtttaaaag aaaatcagtg
     8941 aagtgagatt taatatattg agtgcatcac ttcaagtgac accctcaata aatttcttaa
     9001 tctgaccagg tgtgactgta aatcttattg gacgaggggg ggctaccaca ccaactattg
     9061 aaaatccttg cggaatctgt tacaacgttt ctaatgatgg atcaactact gatataggtt
     9121 caaaccgcga agagtgtcta aaattaatcc aaccaacata tactcccagt acttcgttaa
     9181 aggtattttc taataatatt gaaaatgggg aatattactc agcaatatat ttagaaaaat
     9241 ccagctttaa tacaggagat acctttagcg ttgttattaa tggaatgtca gctaccgcat
     9301 ttgcattaaa gggtgaaagt aacagtacgg ttgtaaataa gctcaaaaat gcaataatta
     9361 gtgttgacaa tggagctcgg gcagaagtag atgataataa ttatttatat gttatctacc
     9421 ccaatagata tgtatatcaa agtgatttaa tcgcaaaatt tgcttcagtt acaagcagtt
     9481 accatgtatg ggttccgtgt ctcgatgact acaccgtgag ggtatatgct aataatgaaa
     9541 gaccttctat aactgtgaat gaaagtgaac cagtatatat cacttgggat tcatttgata
     9601 cagcaaagag ctgtaattgt agttatataa atgatgatgg tctaactaaa ttttgtgg
//
LOCUS       DAORKP010000089         9468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_980_length_9468_cov_9.316052, whole genome shotgun sequence.
ACCESSION   DAORKP010000089 DAORKP010000000
VERSION     DAORKP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_9468_cov_9.316052"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..740)
                     /locus_tag="PLN75_06200"
     CDS             complement(177..740)
                     /locus_tag="PLN75_06200"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRG03741.1"
                     /translation="MTDCSWHANDPLMKAYHDTEWGVPLHDDRKLFEFMVLDAFQAGL
                     SWRTVLYKRENFRRAFDQFDPVKIASYPESKIRELMEDASIIRNQAKIRATVTNAAAF
                     LRVVTEFGSFDRFIWQFTGYQTIHHHYQSISEVPASTPESDAMSKALQQRGFKFVGTT
                     ICYAFMQASGMVNDHTESCEVNPLRFG"
     gene            845..1381
                     /locus_tag="PLN75_06205"
     CDS             845..1381
                     /locus_tag="PLN75_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRG03742.1"
                     /translation="MNPIEIIQKYYDKKSKLYKILLKHSCQVRDKALEVVDRHPEFGA
                     DRQFVSDAAMLHDIGVYLTDAPEIECFGTHKYVAHGYLGSEIVAREGYPQHALVCERH
                     TGLGLSLEWIEKRNLPVPHRDMRPVTIEEQIVCYADKFYSKTKLSRELKVDKILKKLG
                     KYDESQVEQFLEWHKRFG"
     gene            1558..1755
                     /locus_tag="PLN75_06210"
     CDS             1558..1755
                     /locus_tag="PLN75_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRG03743.1"
                     /translation="MFTERIGSNAGLIWAALENGEMEVKSLKKATKLTEKDLNLALGW
                     LAREGKVSFREVEKDLYVALA"
     gene            complement(1836..3788)
                     /locus_tag="PLN75_06215"
     CDS             complement(1836..3788)
                     /locus_tag="PLN75_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRG03744.1"
                     /translation="MKTLFLFFSLLFSISLVAQNAKPFVIPELKEWKASDGVLLFCPK
                     IKIVSDEASNQVARQFAADYKDMFGKKLKLHAGRSNSAGVISFTIHPDSLKANGEEAY
                     SIDIGTSVQVTANSTTGLYWATRTLLQMLEQSHSLPKGTITDFPDYPKRGFMLDVGRK
                     FFPMEYLQAVVRLMSYYKMNTFQVHLNDNGFIQHFGNDWSKTYAAFRLESETYPGLAA
                     KDGHYSKAEFRRFQQDALAQGVTVIPEIDAPAHTLPFTHYLPEIGSKEYGMDHLDLFN
                     PKTYTFLDGLFKEYLEGPNPVFVNEYVHIGTDEYSNKDKAVVEKFRYFTDYYIKEVEK
                     YGKKAALWGALTHAKGDTPVKVKDVLMNCWYNGYAQPRDMMKLGYDVMSIPDGLVYIV
                     PAAGYYYDYLNTKHLYENWTPAVIGGETFEEKHPQIKGGMFAVWNDHPGNGISTQDVY
                     HRIYPAMQTLAVKMWTGRKVELPFAEFDAKRKLLSEAPGQNMLSTPAKSEKGIVFEIR
                     KPTQGEPLKQKLTDIGWNYRTSFDVTAGRNPNGTALFISDNSVFYLTAPADGKLGFSR
                     NGYDYQFNYTVPEGQKVHISIEGTHMETSLYVNGKLQETLRVIPHPDDVNVKKDKRKR
                     VQTLVFPLKETGKFNGKVENLKVEFI"
     gene            complement(4137..4736)
                     /locus_tag="PLN75_06220"
     CDS             complement(4137..4736)
                     /locus_tag="PLN75_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRG03745.1"
                     /translation="MNAEIITAVALGIGLSASAGFRVFIPMLVASVAAHFNIIPLSDS
                     FAWLGSWPAMISFGVATVVEVLAYYIPFVDNLLDTITTPMAVGAGTLLMTSVLPVDNE
                     LMKWIMGFVVGGGAAATMQSGSALSRFASSKFTAGTANPVVSTGENVVALGASVSALA
                     IPVVVAVIFIIIAILIFSRMAAWRRHKKARQREAGQSGD"
     gene            complement(4910..7339)
                     /gene="lon"
                     /locus_tag="PLN75_06225"
     CDS             complement(4910..7339)
                     /gene="lon"
                     /locus_tag="PLN75_06225"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRG03746.1"
                     /translation="MKKQFDNLFTNEMMGESEVYPIISLDENPTEINLNPGDELPILP
                     LRNMVIYPGVLVPVSVARPKSLRLIRQAQANDGLIGACTQIDKKVEDPTIDQLYKMGV
                     AAHIVRVLEMPDSTTTVILEGKKRFRLGELVSNKPYLKAKAYPVDDVLPDTSDKSFIA
                     LSSTIRDLAVKIIEGSGIVPPEMAFAIRNIENPVFLINYICTNFGINVEEKQRLLEID
                     DVMERGYQLLNLLNKEFQMIEIKTSIQNKVKEGIDQQQREYFLQQQMKTIQSELGENS
                     PEYDVQEFRERARSKKWTAPVGKIFEKELRKLERMSQHSPEYTVQLNYVETMLDIPWG
                     EYTKDNFNLKNAKKVLEKDHFGMDTVKERILEHLAVLKLKGDMKSPIICLYGPPGVGK
                     TSLGKSIAAALGRKYVRMSLGGLHDEAEIRGHRRTYIGAMPGRIMQNIKKAESANPVF
                     VLDEVDKINADYKGDPSSALLEVLDPEQNHAFHDNYLDIDFDLTKVLFIATANNLNAI
                     PRPLLDRMELIEVSGYTQEEKVEIAVRHLVPKELENHGLTTEQVQITKPVVSQVIDQY
                     TRESGVRELDRKITGLMRKVALKVATKEKYNVEITPEDVKTYLGTPRYSRDRYEGNEY
                     AGVVTGLAWTSVGGEILFIESSLSPGKTPALTLTGNLGDVMKESAMLAMQYIKAHAAE
                     LEIDQKKIEENNVHVHIPEGAIPKDGPSAGVTMVTSLVSAFTRRKVRKNVAMTGEITL
                     RGKVLPVGGIKEKILAAKRAGITDIVLCEENRKDIDEIKTVYLKGLKFHYVTDIMEVI
                     NFALLKEQVKK"
     gene            7685..9436
                     /locus_tag="PLN75_06230"
     CDS             7685..9436
                     /locus_tag="PLN75_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRG03747.1"
                     /translation="MRKISGYILLFFSVLFLAVFCKLEGKKSGNHSMSLKKNPPDTTL
                     FQQALNEYLATLDSADQSFYNQIYEPKGMNSFWFSDLKRAAAHIDTLNKVISASSDHG
                     IRPEYFDIKSVVDYTAVLDFKKPDYAQLAVAEIMLTDVYFKYCRGMKFGFINPLKLYP
                     KDYFIQIQQPDSAFVNSIFSNPDSLRVYLDHVVPKNKEYISLQQEMQKLRLLKDSVFP
                     SIPLLPPGKTIMAGKKHTSVPLVARRLMLTGELPYTPKVDSVYRTFNDTLLVALNKFR
                     TKYNLLIDKEIGNGTLAALNRSFDSQYWVVAANLERLRWKPLNDISRKYVHVNVANMT
                     LQALKGDTLVQTMKVVVGKPPKNKTPLLYGKMYEVVLNPTWTVPNSIIINEISRRMRN
                     DPSYLGRNRMKVYRNRMEVSEWSVPWDSLTSRYQPYVIVQDSGSNNSLGRLKFNFSNP
                     HNVYLHDTNNKSAFKLHYRGLSHGCVRVEKPLELAFFCLHDVDSTNSSEVRENNILQD
                     RIRHSIGLRAKTSEGKTALSKGQGLKKLKRVELKPGVTVLLDYHTCFTGKDGDVQFCN
                     DHYKMDSVLVGKLKRLE"
BASE COUNT         2483 a         2112 c         2153 g         2720 t
ORIGIN      
        1 catgttttca tgtcttcatg caatcaagta acactcccgt caatcataaa acgttgttaa
       61 aagttttctt tcgctcactt tggggtttgg ggactgagta gctgcaagcc gatcaaccaa
      121 tcaaccaatt caccaattca ccaattcacc aattcaccaa tcaactaatt cactaatcaa
      181 ccaaacctca acggatttac ctcacaactt tctgtgtggt cgttgaccat accggaagcc
      241 tgcatgaaag cgtagcaaat cgtagtgccc acaaatttga atccccgctg ctggagggct
      301 ttgctcatgg catccgattc gggtgtgctg gccggaactt cggatatgga ctgatagtga
      361 tggtggatgg tctgatagcc ggtaaactgc cagataaacc ggtcgaacga accaaactcc
      421 gtgaccacac gcagaaaagc agcggcgttg gttacggtgg cgcgtatttt ggcctggtta
      481 cgtatgatgg aagcgtcctc catcaactcc cggattttac tttcggggta cgatgcaatt
      541 ttcacgggat caaactgatc aaacgccctg cggaaattct cccgcttgta gagcactgtt
      601 cgccagctca accctgcctg gaaggcatcg agcaccatga attcaaacag cttgcggtcg
      661 tcgtgcagcg gaacacccca ttcggtgtcg tgataggctt tcatcagcgg atcgttggcg
      721 tgccacgaac aatctgttat ttctattgtt ttttttacta aatttgccac cgtttttttt
      781 gttagttgca caaaattaca aattcaaatt gagaattgag tgtaattcgt ttataaaata
      841 ttgcatgaat ccgatcgaaa tcattcagaa atactacgat aaaaagagta aactgtataa
      901 aattcttctg aaacacagct gtcaggtgcg cgataaggca ctggaagtgg ttgaccgaca
      961 tcccgagttc ggagcagacc gccagtttgt atccgatgct gccatgctgc atgatatcgg
     1021 cgtgtacctt accgatgcgc ccgagatcga atgcttcggc actcataaat acgtggctca
     1081 cggttacctg ggttccgaaa ttgtagcccg cgaaggttat ccgcagcatg cgctggtctg
     1141 cgagcggcat acgggcctcg gactgagcct cgaatggatc gaaaagcgca atctgcctgt
     1201 tcctcaccgc gatatgcgcc ccgttaccat cgaagagcag attgtatgtt atgccgataa
     1261 gttttactct aaaaccaaac tcagccgcga actgaaagtg gataaaatac tgaaaaaact
     1321 gggcaagtat gacgagtcgc aggtggagca atttctcgag tggcacaaac ggtttggata
     1381 aatttacatt tatcggaacg aaagtgatgg gcaatggctt agttaaagaa ggttaaatta
     1441 attaaaaagt aagctgatat tcagtgtttt tatgttttac aacatttttt taggtaactt
     1501 tgcaccgaaa attaaagaat tgaaaaattt aaattcatac taaatttata gaaaattatg
     1561 tttacagaaa gaattggttc aaatgcaggc ttgatctggg ctgctttgga aaatggagaa
     1621 atggaagtga aatcgcttaa aaaagcaacc aaattgacag aaaaagattt gaatcttgct
     1681 ttgggatggt tggcacgcga aggtaaagtg tcattcagag aagtggagaa agatctttac
     1741 gttgcgctgg cataacacag aaagtaatac gtataaaaaa agcggaacag ttcacagaac
     1801 tgttccgctt tttttggtat aacatccggc tgtttctata taaattccac tttcagattt
     1861 tccacttttc cgttgaattt acctgtttct ttcagcggga aaaccagcgt ttgcactcgt
     1921 ttgcgcttgt ctttttttac attcacatcg tcagggtggg gtattacacg gagggtttct
     1981 tgaagcttac cgttcacata aagcgatgtt tccatgtgcg taccttcaat gctgatatgt
     2041 actttctgtc cttcgggcac ggtgtaatta aactgataat catatccgtt gcgactgaaa
     2101 cccagtttgc cgtcagcagg agcagtgagg tagaataccg aattatcgga gataaacaaa
     2161 gctgttccat tcggattcct gccggctgta acatcgaaac ttgtccggta attccagccg
     2221 atatcggtga gtttttgttt cagtggttct ccctgcgttg gttttctgat ttcaaacaca
     2281 attccttttt cggattttgc aggtgtggag agcatgttct gtccgggtgc ttcactcagc
     2341 agcttccttt tagcatcaaa ctcagcaaac ggcagttcga ccttcctgcc tgtccacatt
     2401 ttcacggcaa gtgtttgcat ggccggataa atacggtgat aaacatcctg agtgctgatt
     2461 ccattgccag ggtggtcgtt ccatacggca aacatacctc ctttaatctg cggatgtttc
     2521 tcctcaaaag tttctccgcc aatcactgcg ggtgtccagt tttcgtacag atgtttcgtg
     2581 ttcaggtaat cataataata gcctgcagca ggcactatgt aaaccaatcc atcgggtatg
     2641 ctcatcacat cgtagcccag tttcatcatg tcgcgaggct gcgcatagcc gttgtaccag
     2701 cagttcatca gcacatcttt cacctttacg ggagtatcgc ctttggcatg cgtaagtgct
     2761 ccccagagtg ccgctttttt gccgtatttt tccacttctt tgatgtaata atcggtaaaa
     2821 taacggaatt tctccaccac agccttgtct ttgttgctat actcatcggt gccgatatgc
     2881 acatattcat ttacaaaaac cggattcggg ccttccaggt attctttgaa aagtccgtcc
     2941 aggaatgtgt atgttttcgg attgaataaa tccaaatggt ccattccgta ttccttgctg
     3001 cctatttcgg gcaaatagtg ggtgaatggg agtgtgtgtg ccggagcgtc aatttcagga
     3061 atgacagtta caccctgcgc aagtgcatcc tgctgaaacc ggcggaattc cgctttgctg
     3121 taatgtccgt ctttagcagc caggccggga taggtttcac tttccagacg gaatgcagcg
     3181 tacgttttgc tccagtcgtt gccgaaatgc tgtataaaac cgttgtcgtt cagatgcacc
     3241 tgaaaagtgt tcatcttgta atacgacatc agtcttacta ctgcctgcag gtattccatc
     3301 ggaaaaaact tacggccaac gtcgagcata aatccgcgct tcggataatc gggaaaatcg
     3361 gtgatagtac ctttgggaag cgagtgcgac tgttccagca tctgcagcaa tgtgcgcgta
     3421 gcccagtaca gtccggttgt gctgtttgcc gtcacctgca ccgatgtgcc gatgtcaatg
     3481 ctgtaagctt cttcaccgtt cgctttcaga gagtcaggat gaattgtaaa cgaaataact
     3541 ccggctgaat ttgaacgacc ggcatgaagc tttaattttt tgccgaacat atctttgtag
     3601 tcggctgcaa actgacgggc tacctgatta gaggcttcgt cagaaacgat ttttattttc
     3661 gggcaaaaca gcaatacccc atcgctggct ttccattctt tgagttcggg gatgacaaaa
     3721 ggttttgcgt tttgggcaac aagggatatt gaaaaaagga gtgaaaagaa taaaaacaag
     3781 gttttcatag catcaattgt gatttttagt taagctggtt tctgtcagta cctttcggta
     3841 caaaatcaga aaagcagggt acaaatatac ggctaaaatc gttatcagta agccctcaga
     3901 ctcttttacg aagcaatcat taacgaaatc aaactttttt ttgaaaaaaa aattgagttg
     3961 tcgtgacaac cggatgccgg tatgcggttg tgaaatgtgg cttttcaatc tttcgggaga
     4021 aacaaagcat acccggataa aaaaacagct caaaacggat ggtttcgagc tatggataac
     4081 gactttagag aaatgaaatg cattccggga aagggataat aacttaccat cgcggattaa
     4141 tccccgcttt gtccggcttc tctttgtctg gcctttttgt gtcttcgcca tgcggccatc
     4201 ctgctgaaaa tgaggatagc gataatgata aagattacag ctacaactac cggaatggca
     4261 agtgccgata cagatgcgcc cagtgccacc acattttcac cggtggaaac aacaggatta
     4321 gctgtgccgg cggtgaattt gctggaagcg aaacgtgaaa gagcacttcc gctctgcatg
     4381 gttgcagcag ctccaccacc caccacaaag cccatgatcc acttcatgag ttcgttgtca
     4441 accggcagaa cagatgtcat cagcagcgta ccggcgccca cagccatcgg agtggtaatg
     4501 gtatccagca ggttatccac aaagggaatg taataagcca gcacctctac cacagtagcc
     4561 acaccaaaac tgatcatagc aggccagctt ccaagccagg caaaactgtc ggaaagagga
     4621 ataatattga agtgggccgc cacactggca accagcatag ggataaatac ccgaaaaccg
     4681 gcactggcac ttaagccaat ccccaaagca acggcagtaa taatttcagc attcataact
     4741 tggcaaatgt agttaaattt ttgaaaatca gaaaaaaatt gtcggttttt ttgaaactca
     4801 aacaatactc ctgcaaatat cgtgcatgtt tttgaatgct gggaactgat ataaaaaaac
     4861 tgcggttgtg atttaaccgc agtttttttg tagctaactt cagggagttt tatttcttta
     4921 cctgttcttt cagtaaggca aaattaatca cctccataat atccgttacg tagtgaaatt
     4981 ttaagccttt caggtaaacg gtttttattt catctatgtc tttccggttt tcttcgcaca
     5041 atacgatatc ggtgatgccg gcacgttttg cagccagaat tttctctttg ataccgccta
     5101 cggggagcac tttgccgcgc agggttatct caccggtcat ggccacattt tttctcactt
     5161 ttcgtctggt gaaggctgaa accagcgatg tcaccatcgt tacgcctgcc gagggtccgt
     5221 ccttggggat ggctccctcc ggaatgtgta catgtacgtt gttttcttct atctttttct
     5281 gatctatttc cagttcggca gcatgagctt tgatgtactg catggcgagc atggccgatt
     5341 ctttcatcac atctcccagg ttaccagtga gggtgagtgc cggagtcttt cccgggctca
     5401 ggctgctttc gataaagagt atctcgccgc ccacgcttgt ccacgcaagc cctgtaacta
     5461 ctccggcata ttcgttgcct tcgtatctgt cgcggctgta tcggggtgta ccgagatagg
     5521 ttttcacatc ttccggtgta atttctacgt tgtatttctc ctttgtagct actttcaatg
     5581 ctacttttcg catcagtccg gttattttcc ggtccagttc gcgcactccc gattcgcggg
     5641 tgtactgatc aataacctgg cttaccacgg gtttggtgat ttgcacctgt tcggtggtca
     5701 gtccgtggtt ttcaagttct tttggcacca ggtgacgaac cgcaatttcc actttttcct
     5761 cctgggtata tccgcttact tcaataagtt ccatcctgtc gagcagcggt cgcggtatgg
     5821 cgttcaggtt gttggcagtg gcaatgaaaa gtaccttggt caggtcgaaa tcaatgtcca
     5881 ggtaattatc gtgaaatgca tgattttgct ccggatcaag tacttcgagc aaagccgacg
     5941 acggatcgcc tttataatcg gcgttgattt tgtccacttc gtccagtacg aaaacgggat
     6001 tggccgattc ggcttttttg atgttttgca tgatgcggcc gggcatggcg ccgatgtatg
     6061 tgcggcgatg accgcgaatc tccgcttcgt cgtgcagtcc accgagcgac atacgcacgt
     6121 attttcggcc cagtgcagct gcaatggact tgcccagcga ggttttgccc acacccggag
     6181 gtccgtacag gcaaataatc ggcgatttca tgtcgccttt caacttaagt acagccaggt
     6241 gttccagtat gcgttctttc accgtatcca tgccgaaatg gtctttttcc agtacctttt
     6301 tggcattttt caggttgaag ttgtctttgg tgtattctcc ccagggaata tcgagcatgg
     6361 tttccacgta gttgagctgc acagtgtatt ccggcgaatg ctggctcatg cgctccagtt
     6421 tacggagctc tttttcgaat atcttaccta ccggtgcagt ccattttttg cttcgggcac
     6481 gttctctgaa ttcctgcacg tcgtattcgg gtgagttttc gccgagttcg ctctggatgg
     6541 ttttcatctg ctgctgcagg aagtattcgc gctgctgctg gtcaatgccc tctttcactt
     6601 tattctgaat ggaggttttt atttcaatca tttgaaattc cttgttcagc aggttgagta
     6661 gctgataacc ccgttccatc acatcgtcga tttcgagcag ccgctgcttt tcttccacat
     6721 tgatcccgaa gttggtgcag atgtagttaa taaggaaaac cggattttcg atatttcgga
     6781 tggcaaaagc catttcggga ggcacaatgc ctgaaccttc gatgattttt acagccagat
     6841 cgcgaatggt ggatgagagg gcgatgaatg atttgtcgga tgtgtcgggc agcacgtcgt
     6901 caacaggata agctttggct ttcaggtaag gcttgttgct caccagttcg cccaggcgga
     6961 aacgtttttt cccttccaga attacggtgg tagtactgtc gggcatttcg agcacacgta
     7021 cgatatgtgc tgccacaccc attttataga gttgatcgat ggtgggatct tctacctttt
     7081 tatcaatctg tgtgcaggca cctatcagcc cgtcgttggc ctgagcctgg cggataagcc
     7141 gcagcgattt ggggcgtgct accgaaaccg gaaccagtac gcccggataa ataaccatat
     7201 tgcgaagggg cagaatgggt aattcatcgc ccggattgag attgatttct gtagggtttt
     7261 catccagtga aataatcgga tatacttccg attcacccat catttcattt gtaaatagat
     7321 tgtcgaactg ctttttcatc tgacgttttt tctcttttat actctttcaa actgccaaaa
     7381 tggcatacgt tcaatttctc tgattgtttg gaaattgaat aacctcttga atatcttagt
     7441 tttagtagta ttgtggttag tgtgtcttat ttttaagtca attgttgtgc caaagacata
     7501 tccgggcttt ttgtcattaa aaaaggtata tatctgaaaa agtcgccgaa tacccataaa
     7561 atgataatat aaggaaacag taacttgttt atcaatcaat gaattttttc cattcatttt
     7621 cagacaaata atggataaat aataaaaaat atgctatttt tgcttaattt ttcattcttg
     7681 tgatatgaga aaaatttccg gctatattct gttgtttttt tctgtacttt ttctcgctgt
     7741 tttttgtaaa ctggaaggga aaaagagcgg aaatcattcc atgagcctga aaaaaaatcc
     7801 tcccgatacc actttgtttc agcaagcgct caacgaatat ctcgctaccc tggattcggc
     7861 agatcagtca ttttataatc agatttatga gcctaagggg atgaattctt tctggttttc
     7921 ggacctgaaa cgcgctgcag ctcatatcga tacactgaac aaggtgattt cagcttcgtc
     7981 agatcacggc atccggccgg agtatttcga tataaaaagc gtagtggatt acacagctgt
     8041 acttgatttc aaaaaaccgg attatgctca acttgcagta gccgagatta tgcttacgga
     8101 tgtatatttt aagtattgca ggggcatgaa attcggattc atcaatccgc tgaagcttta
     8161 tccgaaagac tattttattc agatacaaca accggacagc gcttttgtga attcaatatt
     8221 ttccaaccca gactcactgc gtgtttatct ggatcatgtt gtcccgaaaa acaaagaata
     8281 catctccctt cagcaagaaa tgcaaaaact cagactactg aaggattcgg tttttccctc
     8341 cattccgctg ctgcctcccg gtaaaaccat catggcaggt aagaaacata cttcggtgcc
     8401 gctcgtggcg cgccggctga tgctaaccgg agaattacct tatacaccca aagtcgattc
     8461 ggtgtacaga acgttcaatg acacgcttct ggtggcactc aataaattca gaacaaaata
     8521 caatctgctg atagataaag agataggaaa tggaaccctc gctgcactca accggagttt
     8581 cgattcgcag tactgggtag tggcggccaa tctggagcgg cttcgctgga aaccgctgaa
     8641 cgatatttca agaaaatacg tgcatgtgaa cgttgcaaat atgactttgc aggctttgaa
     8701 aggcgataca ctggtgcaga ccatgaaagt ggtggtgggt aaacctccca aaaacaaaac
     8761 tccgctactg tacggaaaaa tgtatgaggt ggtgctcaac cctacatgga cagtgcctaa
     8821 cagcatcatc atcaacgaaa taagcagacg gatgagaaat gacccatcct acctgggcag
     8881 aaaccgcatg aaggtgtacc gcaacagaat ggaggtgagc gaatggtcgg taccgtggga
     8941 cagcctgacc agcaggtatc agccttatgt aatagtgcag gattccggat caaacaattc
     9001 gctggggagg ctcaaattta atttttccaa tccgcacaat gtatatctgc acgatacaaa
     9061 caataaatcg gctttcaaac tccattatcg tggattgagt cacggctgtg taagggtgga
     9121 gaaacccctg gaacttgcat ttttttgcct gcacgatgta gattcaacga acagtagtga
     9181 ggtgagagaa aacaatatat tgcaggaccg catcaggcat tctatcggac tgcgtgccaa
     9241 aacttccgaa ggaaaaacag cattatcaaa aggacaggga ctgaaaaagc ttaaaagggt
     9301 ggagttgaag cccggagtta ccgtgctgct tgattatcac acatgtttca ccggaaaaga
     9361 tggcgatgta caattctgca atgatcatta taaaatggac tcggtgcttg taggcaaatt
     9421 gaagagactc gaataaattt cacgttccgt gttccgtgtt ctgtgttc
//
LOCUS       DAORKP010000090         9446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_985_length_9446_cov_8.416037, whole genome shotgun sequence.
ACCESSION   DAORKP010000090 DAORKP010000000
VERSION     DAORKP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_9446_cov_8.416037"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3046)
                     /locus_tag="PLN75_06235"
     CDS             complement(<1..3046)
                     /locus_tag="PLN75_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03748.1"
                     /translation="MGFAQKVSLNYSQQSLKNVLQSISRQTGYTLAYSKEVVDLNDNV
                     SIQVQNADLSVAMAKLFASRNLSYEIKDKKIYIADNSPRTEARSTASQQTENVQIRGK
                     VTDENGEPVIGASVAVPNTGIGTITNIDGDYSLSVPRGSLLRISFVGYVNQEFTITNQ
                     SVLNAQLREDVASLDELIVIGYGVQKKSMVTAAISRVTAEDLAQSKPSRIEDVLKGKV
                     SGVQITQSSGQPGADSKVRIRGIGTINNSDPLYIVDGMPVDGGIAYLNPADIQSVEIL
                     KDAASAAIYGTRAANGVVLITTKTGSVSRGSINYNFSYGWQNPWKKKRMLNATEYMTL
                     MNEAALNDGGIPVYGANAFEKLGGAGTDWQDATFNYDAPIQSHQISASGGSQNAQYFL
                     SAGYFSQEGVIGGNFGRSNYDRFSVRSNTTFAAYETKERSFLNKLDVGVNMGYSRVKS
                     TGVEANSEYGSILGSAIGSSPLSPIYAPETSEFDFIYTQSYIENKYPTAVRDAEGRLF
                     SIPTSVFQEIANPVAMLNQPGMGRNNEDKFVGTFWGELSLLPGLKFKSSFGTDLAFWG
                     YDSYNFPYFMTTQGKEIQRSDVQSEMNRGMKWQLENVLSFNKTFNEVHNFSAVLGQSA
                     IRYSTRNLGGYTKDLLMLDPVMANINSGTADKIDQAVWGGTNGFDYESLASYFGRVIY
                     NFDERYMAQASVRRDGSSRFGPNNKWAIFPAFSLGWNVHNESFMENRPDWFNALKIRY
                     SWGSNGNERMPNFRYVSRLDGGQNYYYGGGYIVDSPTTGTINGTMQYGSSPAGLPNPS
                     LKWEHSVQSDFGFDAQFFNSALTFSFDYYTKKTNGMLIQVPIPAYVGQGSPWGNVGDM
                     KNSGLEFELGWKQVVNDFKYHISANASYAKNVLVNLGNATGETILESAGASGVGSYVK
                     GKNGEVYPYFYGFKTDGIFQNQTEVDTYVNADGVKYQPQAKPGDVRFVDFDGDGVITD
                     DDKTKIGKGMPDWTFGSTMGAEWKGFDVNFFFQGTYGNDMYDF"
     gene            complement(3358..4347)
                     /locus_tag="PLN75_06240"
     CDS             complement(3358..4347)
                     /locus_tag="PLN75_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRG03749.1"
                     /translation="MNTPDKELIDRVIAGQATKKEAREVVEWFSDTTEGQSYLSELIN
                     SDAHLLDLEGVDETSLPPMLSQKILAEINRQIRQKEIRHLIFRVAAILIPFVMIAGLT
                     FYTNSRLDLFGTSGYSEIYIPKGEKTRILFQDGSQAFLNADTKIRYPEKFGLTERKIQ
                     LEGEAYFKIATNSNRPFIVQINDTKVKVLGTSFNVKAYKNDKNLNIVLDRGKISFITP
                     RNSYNLHPGQQAVYNTESGQCSIYSLDKSTEASLWVNNITQFKDTPLPEVLKALNRKF
                     NVEFRLNDPNAYKYTYTLTTESNSLKEIIKELEKITPVRFQIQNDNVVIVTTN"
     gene            complement(4384..5001)
                     /locus_tag="PLN75_06245"
     CDS             complement(4384..5001)
                     /locus_tag="PLN75_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRG03750.1"
                     /translation="MSKNNPLHINNEDEVLFHLIKKKDKSAFTAIYNKYHRYLYALSV
                     RYLKNSEMAEEVVQYVFVKLWETITDIDIQINLKNYLYTMTKNYILNTIRNKKEMVSL
                     SYEKSQIEIKDETGDFLKMIEESQLMSILHKGIDSLPPQKREICRKKINENKSNQEIA
                     EEMGVSIHTVKSHYQESIRLLRIYFGKFESIFLLISIPELILLLS"
     gene            complement(5244..5624)
                     /locus_tag="PLN75_06250"
     CDS             complement(5244..5624)
                     /locus_tag="PLN75_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRG03751.1"
                     /translation="MEISGKIIAVLPLQSGQGKNGLWRSQDYVLETADQYPKKVCFNL
                     FNDKIDKFPMALNEMVTVSFDIESREYSGRWYTTIRAWNVKKNTEDNPAEGVQSSEIP
                     PFSATKHPSFGLPDDSENITDLPF"
     gene            complement(5774..7198)
                     /locus_tag="PLN75_06255"
     CDS             complement(5774..7198)
                     /locus_tag="PLN75_06255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRG03752.1"
                     /translation="MDYISNFLYMRSEVSLLAVIIFLLLYDLIAGEKGMKYFQPVAII
                     VFAVHTLATIFPYEAGIAFGGMYQYSPMMTIVKGILNLGTLIVFLQAKEVFDGDKQSN
                     KRGEYYFLTLITLLGMYFMISSGHFLMFYIGLEMASIPLATLAALEKYSKKSAEAGAK
                     YILIAAFSSGIMLFGISYLYGSVGTLYFADMPVHITANVLQIMAFVFFMVGLFFKISL
                     VPFHLWTADVYEGAPTSVTSYLSVVSKGSAVFVLMILLYKVFGNLMVQWQGILYGIII
                     VSITLANLFAIRQQNIKRFLAFSSISQAGYIALAIISGTAFGMTTLVYYIFVYVFSNL
                     AAFGVVTTIENRSGKLNISDYKGLYSTNPQLSFVMMLAMFSLGGIPPFAGFFSKFFVF
                     AAALEQGFYVLVLIAVVNTIISLYYYLLVVKAMFIDSSENPVPMLKSDFFNRTSLFIC
                     TAGILMVGILSFFMDKIGLYSFGM"
     gene            complement(7336..8829)
                     /locus_tag="PLN75_06260"
     CDS             complement(7336..8829)
                     /locus_tag="PLN75_06260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRG03753.1"
                     /translation="MNFLLIFVLIPVLMTLTLFLARNRKQVLGIMVTGSIALVVASAY
                     LVVVYVSARNAGASDPMLFTSHLMWYKPLNIGFSLGVDGVSVAMLCLSSIIVLTGTFT
                     SWQIAPLTKEFFLWFCLLSTGVFGFFITTDLFTMFMFYEVALIPMYLLIGIWGSGKKE
                     YSAMKLTLMLMGASAFLLVGILGIYFSAGATSMNILDIVKVHIPIEQQYLLFTLTFFG
                     FGVLGALFPFHTWSPDGHASAPTAVSMLHAGVLMKLGGYGCFRVSIYLMPEAAEVLSP
                     IFLILTGTGVFYGALAAIHQRDLKYINAYSSVSHCNLVLFAILMMTQTAMTGAVLQML
                     SHGLMTALFFALIGMIYGRTHTRMITEMGGLMKVIPFLGASFVIGGLASLGLPGLSGF
                     AAEMTIFIGSFENADLFRRILTIIAISSIVTTAVYILRMVGKFLYGPMQDEHHAEITD
                     AKWFEKLAVITLIAGIVYIGSFPLGLSDMLHDSLGNVMAQINGPVAP"
     gene            complement(8871..>9446)
                     /locus_tag="PLN75_06265"
     CDS             complement(8871..>9446)
                     /locus_tag="PLN75_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRG03754.1"
                     /translation="AFYMFRLYYNIFWGKEYKIHGNHKPHESPRSMTYPLMFLALLTI
                     VGGFIPFGKFVTPDRSEYLIHLDIKIAAISVAVALAAIALATVLYRRDSKRPAELAGK
                     MRGVYTVAYNKFYIDEVYQFVTKKIIFNSISRPFAWFDRHVVDGAMNGMAWLTGWTSL
                     KTKGFQSGSIQWYAWVFLFGTLLITALAAFL"
BASE COUNT         2707 a         1991 c         1980 g         2768 t
ORIGIN      
        1 agaaatcgta catgtcgttt ccgtaggttc cctggaagaa gaaattcacg tcaaaacctt
       61 tccactcagc acccatggtc gaaccaaaag tccagtccgg cataccttta ccgattttgg
      121 ttttgtcgtc atcggtaatt acaccgtcac cgtcaaagtc aacaaatctt acatcaccag
      181 gtttggcttg tggctggtat ttaactccgt cggcatttac gtatgtatct acttctgtct
      241 ggttttggaa gattccatcc gttttgaaac cgtagaaata tggataaacc tcaccgtttt
      301 taccttttac gtatgatcct actcctgatg caccggcact ttccaaaata gtttcgccgg
      361 tggcattacc caggttgacc aacacgtttt tagcataaga agcattggca gaaatatggt
      421 atttgaaatc gtttaccact tgtttccatc ctaattcaaa ttccaacccg ctgtttttca
      481 tatcacccac gttgccccag ggagaacctt gacccacgta tgcaggaatt ggcacctgaa
      541 tcaacatacc attggttttt ttggtataat aatcgaagct gaatgtcaat gcgctgttga
      601 aaaattgtgc atcaaaaccg aagtcgctct gtacggagtg ctcccatttc aatgatgggt
      661 ttggaagacc tgccggagaa ctaccgtact gcatagttcc gttaattgtt ccggtagtag
      721 gcgagtcaac gatgtagccg ccgccgtagt agtagttttg accaccatcc aggcgagaaa
      781 catagcggaa gttcggcata cgttcgttac cattggaacc ccagctgtaa cggattttca
      841 atgcattgaa ccaatccggt cggttttcca tgaaagattc gttgtgaaca ttccagccta
      901 aagagaatgc agggaaaata gcccatttgt tattcggacc aaaacgtgaa gaaccgtcac
      961 gacgaacaga agcctgcgcc atgtagcgct cgtcgaagtt gtaaattaca cgaccgaagt
     1021 aagatgccag tgattcgtag tcaaatccgt tggttccacc ccaaactgcc tggtcaattt
     1081 tgtcggcagt tcctgagttg atgtttgcca tgaccggatc aagcatcagc aggtctttag
     1141 tataaccgcc taaattgcgg gtgctgtatc tgatggcaga ttgtcccaaa acagcagaga
     1201 aattgtgaac ttcgttaaat gttttgttaa atgacaatac attttcaagc tgccatttca
     1261 tacctctgtt catttcgctt tgaacgtcgc ttcgctgaat ttcttttccc tgagtggtca
     1321 taaaataggg gaagttgtag ctgtcatagc cccagaatgc aaggtcagta ccgaaagaag
     1381 atttgaattt caagcctgga agaagactta attcacccca gaaagttccc acaaatttgt
     1441 cttcgttgtt tcttcccatt cccggctggt tgagcattgc tacagggtta gcaatttctt
     1501 gaaaaacaga tgtcggtatg gagaataatc ttccttccgc atctcttaca gctgttgggt
     1561 atttattctc aatgtaagat tgagtataga tgaaatcaaa ttctgatgtt tcaggagcat
     1621 aaattggaga tagcggagaa gaaccgatag cactacccag aatggagccg tactcggagt
     1681 tggcttcaac acctgtagat tttacccttg aatagcccat gtttacacct acgtccaatt
     1741 tgttcaggaa agaacgttct ttagtctcgt aagcagcaaa tgtagtattg gaacgtacgc
     1801 tgaaacggtc gtagtttgat ctgccaaagt taccaccgat aacaccttcc tggctgaaat
     1861 aacctgcaga aaggaaatac tgcgcatttt ggcttcctcc gcttgcgctg atttggtgtg
     1921 attggatagg agcgtcgtag ttgaatgtag cgtcttgcca gtctgtaccg gctccgccta
     1981 atttctcaaa ggcatttgcc ccgtacacag ggattccacc gtcattaagc gcagcttcat
     2041 tcatcaaagt catgtattct gttgcgttca gcattctttt tttcttccag ggattttgcc
     2101 atccgtagct gaaattgtag ttgattgatc ccctgcttac cgaaccggtt ttggtggtaa
     2161 tcaacacaac accgtttgca gcacgtgtac cataaatagc agctgaagcg gcgtctttca
     2221 aaatttcaac agactggata tcggcagggt tcaggtatgc gatacctccg tcaaccggca
     2281 ttccatccac gatatagaga ggatcgctgt tgttgatggt accaatacca cgaatacgta
     2341 cttttgaatc cgcgcccggt tgtccggaac tctgtgtgat ctgaactccg gaaacttttc
     2401 ctttcagcac gtcttcgata cgtgatggtt tggactgtgc caggtcttcg gctgttacac
     2461 gactgatggc agcggtaacc atgctctttt tctgtacgcc gtaaccaatt acgataagtt
     2521 cgtcgaggct agccacgtct tcgcgtagct gtgcgttgag tacagattgg tttgtgatgg
     2581 taaattcctg attcacataa ccgacgaaag agatacgcag taatgaacca cggggaactg
     2641 aaagtgaata atcaccatct atattggtga ttgttcctat gccggtattg ggcacagcta
     2701 cgcttgctcc gattaccggt tcgccgtttt catccgttac tttgcctctg atctgtacat
     2761 tttcagtttg ctgcgaggca gttgacctgg cttctgtacg gggtgagttg tcggcaatgt
     2821 aaattttctt gtctttgatt tcgtagctca gatttcttga ggcgaataat ttggccatag
     2881 ctaccgacag atcagcattc tgaacctgaa tacttacatt gtcgttgagg tcaacgactt
     2941 ctttgctgta tgcaagcgta taaccagttt gccggctgat actctgcaat acatttttaa
     3001 gactctgttg gctgtaattc aaacttactt tttgtgcaaa tcccatattg ccgggactca
     3061 acatcaaaaa gcagattaaa ataaaagcca accttttcaa aaggtttttt ctcatggtgc
     3121 gaactttgat ttagttaaac atttatttat tagatacaag aatatttttt tattttctta
     3181 aatccaaaaa agaacttctg ttgctgatat atttcaaaga ataagcggtt tcaatcagtt
     3241 cacaggttaa agttaaggtt tttttttatg taaaactaat ttttatacgg ttattattcg
     3301 tgtttatctt ttttgacacg gactgaacct gaaagtatga gtaagtattg atttttttta
     3361 atttgtagtt actatgacta cgttatcatt ctgaatttga aatctgaccg gtgtgatttt
     3421 ttcgagttcc tttataattt ctttcaggga attgctttcg gttgtcaggg tgtatgtgta
     3481 tttataggcg ttcgggtcat tcagcctgaa ctcaacatta aacttccggt tcagggcttt
     3541 aagtacttcg ggcaacggtg tgtctttgaa ttgtgtgatg ttatttaccc aaagtgaagc
     3601 ttctgttgat ttatcaagag agtatatgga gcattgcccg gattccgtat tataaacggc
     3661 ttgctgaccg gggtggaggt tataactgtt tcggggtgtg ataaacgata ttttgcccct
     3721 gtccagtaca atattcaggt ttttatcgtt tttataagcc ttcacgttga atgaggttcc
     3781 cagtactttc acttttgtgt cgttaatctg aacgataaaa gggcggttgc tgttagtggc
     3841 aattttgaag taagcttcac cttcgagttg tatttttctt tcggtcagtc caaatttttc
     3901 cgggtagcgg attttggtat cggcattcag aaatgcctga cttccatcct gaaataaaat
     3961 gcgggttttt tcgcctttcg gaatgtaaat ttctgaatat ccggatgttc cgaataaatc
     4021 caggcgggaa ttggtgtaga acgtaagccc tgcaatcatt acaaagggga taagtatggc
     4081 tgctacgcga aaaataagat gtctgatttc tttctgacga atttgcctgt tgatttctgc
     4141 gaggattttc tgtgaaagca ttggtggaag agaggtttcg tctacacctt ccaaatcgag
     4201 caggtgagca tcactattta tcaattcgga taaatatgac tgaccttcgg ttgtgtcaga
     4261 aaaccattca actacttcac gggcttcttt tttggttgcc tgacccgcta ttacgcggtc
     4321 tatcagttct ttatcaggtg tattcatggt attgtaattt gcttcttaca atatattgac
     4381 acctcaggac agtaaaagta tgagttcggg aatggaaatt agtagaaata ttgattcaaa
     4441 tttaccaaaa tagattctta gtaacctgat tgattcctga tagtgtgatt ttacggtgtg
     4501 aatggatact cccatctctt ctgcaatttc ctgattgctt ttgttttcgt tgattttctt
     4561 ccggcaaatc tccctttttt gtggtggcag gctatcgata cctttgtgaa ggattgacat
     4621 gagctgtgat tcttcaatca ttttcagaaa atccccggtt tcatccttaa tctctatttg
     4681 tgatttctcg taactgagtg ataccatttc ttttttattc cgtatggtat tgaggatgta
     4741 attttttgtc atcgtataca gataattctt caaatttatc tgtatatcga tgtctgttat
     4801 tgtttcccaa agtttcacaa aaacatactg aacaacttct tcggccattt cagagttctt
     4861 caggtacctt acagacagtg cataaagata tctgtggtat ttgttgtaaa tagcagtaaa
     4921 tgccgatttg tcttttttct taattaaatg aaaaagaact tcatcttcgt tgttgatatg
     4981 taacggatta ttttttgaca tgttatctgc tgaaaatcac agaaatttcg gatcaggtca
     5041 acaaaggtaa ttgtaaaaat agttaatttt taaaaacttc gtatttgagt aattcacaag
     5101 attaaacgaa actttttgag tcttacctga aaaaacagtc gtggcatgca taaatatttg
     5161 taaaaagcac attaacactg tataaaaaaa gaaagctcag ttttctgaaa gttctttaat
     5221 gtctgctctg aagtttatac gattcaaaaa gggagatcgg tgatattttc ggagtcgtcc
     5281 ggtaaaccaa atgagggatg tttcgttgct gaaaaaggag gtatttctga tgattgtact
     5341 ccttctgcag ggttatcttc agtatttttc ttcacattcc acgccctgat ggtggtatac
     5401 catctgccgc tgtattcacg gctttcgata tcgaaactga cagtgaccat ttcgttcaat
     5461 gccatcggaa acttatcaat cttatcgttg aaaaggttga aacaaacctt tttaggatac
     5521 tgatctgcgg tttccaatac gtaatcctgc gaacgccaaa ggccgttttt gccctgtccg
     5581 ctttgcagtg gtagtactgc aataattttt ccggaaattt ccattttttt gctgacttat
     5641 atttgttgta aattttttta taatcgggat gcaaagataa gaaactaaat cgttctccga
     5701 aaagaaatga ctataaaaaa tcccctgcat cagattgata caggggacac ttttttattc
     5761 agattttcga acgttacatt ccaaaactgt acaacccgat tttatccata aagaaactga
     5821 gaatacctac catcagtatg ccggcagtac agataaacag gcttgtacgg ttgaaaaagt
     5881 cggatttcag catcggaacc ggattctcac tgctgtcgat aaacatggct ttcacaacca
     5941 gtaaatagta gtagagtgaa ataatcgtat tcactacagc tatcagcacc agaacataga
     6001 atccttgttc cagggcggct gcaaatacaa aaaacttact gaagaatccg gcaaacggcg
     6061 gaataccacc gagcgaaaac atggcaagca tcatcacaaa acttaattgc ggattagtac
     6121 tgtagagtcc cttgtaatcg cttatattca gtttacccga acggttttcg atggttgtaa
     6181 ccaccccgaa ggcagccaga ttggagaata catacacgaa aatatagtaa accagcgtgg
     6241 tcataccgaa tgcggttcca ctgattatgg ccagcgcaat gtatcctgcc tgagaaatgg
     6301 aagagaatgc caagaaacgt ttgatgttct gctgacgaat ggcaaacagg ttggccagcg
     6361 tgatactcac aatgattatt ccataaagaa tgccctgcca ctgaaccatc agattaccga
     6421 aaaccttgta aagcagtatc atcagcacga atacggctga acctttggat accactgaca
     6481 gatacgatgt aacactggta ggtgcgcctt catatacatc ggcagtccag aggtggaaag
     6541 gcaccagtga aatcttgaaa aacaaaccta ccataaagaa aacgaacgcc atgatttgaa
     6601 gcacgttggc ggtgatgtga actggcatat cagcgaaata cagcgtacct accgaaccat
     6661 atagatacga aatcccgaag agcatgattc cggatgagaa ggcagcaatg aggatgtatt
     6721 ttgcacctgc ttcggctgat ttcttgctgt atttttcaag ggctgcaagt gtggcaagcg
     6781 gaatcgaggc catttccaga ccgatgtaaa acatcaggaa gtgtccgctc gaaatcataa
     6841 aatacattcc aagcagcgtt atcagcgtaa ggaaatagta ttcgccacgt ttgttgcttt
     6901 gcttgtcacc gtcgaatact tcttttgcct gcagaaatac aatcagcgta cccaggttca
     6961 gtatgccttt tacgattgtc atcatcggtg aatactgata cattccgccg aaagcaattc
     7021 ctgcctcgta aggaaatatc gttgcaaggg tatgcactgc aaaaacgatg atagcaaccg
     7081 gctgaaaata cttcattccc ttttcgccgg caattaaatc ataaagcagc agaaaaataa
     7141 ttacagccaa aagcgagact tcgctgcgca tgtataaaaa attactaata tagtccataa
     7201 ctttatgccc ataacctcct gaagcgggat tattttgggg ttatttaatg tttgttttat
     7261 taaaagaatc tatctgatag aaaaaatcat ttttaattat tcagcaaagg gctgaaattc
     7321 agtgctaatt cccctttagg gggctacggg gccgtttatc tgcgccatca catttcccaa
     7381 actatcgtga agcatatccg acaatcccaa agggaatgaa ccgatatata cgatacctgc
     7441 aatcagcgta attactgcca gtttctcaaa ccatttcgca tcggttatct cagcatggtg
     7501 ctcatcctgc atgggtccat acaggaattt tcccaccatc cgaagaatgt aaactgctgt
     7561 agtaacaata gatgaaatgg caatgatagt aagaatacgg cggaaaagat cggcattttc
     7621 aaatgaaccg ataaaaatag tcatctctgc cgcaaaaccg cttaagcccg gaagtcccag
     7681 tgatgccaac ccgccaatta caaatgaagc accgaggaaa ggaataactt tcatcagacc
     7741 acccatttcg gttatcatac gtgtatgtgt gcgaccgtaa atcataccaa tcagtgcaaa
     7801 gaacaaggct gtcatcagac cgtgtgacag catctgaagt accgcacccg tcatggctgt
     7861 ctgagtcatc atcaggatgg caaacagcac caggttacag tggcttaccg atgaatatgc
     7921 attgatgtat ttcaaatcgc gctgatgaat ggctgccagt gccccgtaaa atactccggt
     7981 accggttagg atgaggaata taggcgaaag aacttctgct gcttcgggca tcaggtagat
     8041 ggatacacgg aaacagccat aaccacctaa tttcatcaaa actccagcat ggagcatgga
     8101 tactgctgtt ggtgcagatg cgtgaccgtc gggagaccag gtgtggaatg ggaaaagggc
     8161 tccaagcacc ccaaaaccaa agaaagtgag tgtgaataac aaatattgct gctcaatcgg
     8221 aatgtgaact ttcacaatat ctaaaatatt catgctggta gcacctgcgc tgaaataaat
     8281 acccagaatg ccaacgagca gaaatgcgga tgcacccatc agcatcagcg taagtttcat
     8341 ggcagaatat tctttcttac cactacccca gatgcctata agcaggtaca tcgggatgag
     8401 agccacttca taaaacatga acatggtaaa caaatcggtg gtgatgaaaa atccgaatac
     8461 gcccgtagaa agcaggcaga accacagaaa gaattctttg gtcagcgggg ctatttgcca
     8521 ggaagtaaat gtaccggtca gcacaataat agaagaaaga cacagcatag ccaccgatac
     8581 accgtcaaca cccagcgaaa agccgatgtt cagcggttta taccacatca gatgagaagt
     8641 gaaaagcatc ggatcggatg caccggcatt acgggcagaa acataaacta ccacaagata
     8701 tgccgaagca accaccagtg caatactgcc ggtcaccatt atacccagta cctgttttcg
     8761 gtttcgtgcc agaaacagcg taagtgtcat cagcaccggt ataagtacga aaataagaag
     8821 aaagttcata tcttaacgtt ataaatttca aattttaaaa aatcagttac ctataaaaaa
     8881 gctgccagtg cagtaatcaa taatgttcca aaaaggaaaa cccacgcata ccactgaata
     8941 cttcctgact ggaatccttt ggttttcaac gatgtccatc cggtaagcca ggccattccg
     9001 ttcatggctc cgtccaccac atggcggtcg aaccaggcaa acggacgtga tatgctgttg
     9061 aaaattattt tcttggtaac aaactgatac acctcgtcga tataaaactt attgtaagca
     9121 actgtgtaca caccgcgcat ttttcctgcc agttctgccg gacgtttgct gtctctgcga
     9181 tatagtacgg ttgccagcgc aatagctgcc agcgcaacag ccacactgat ggcagcgatt
     9241 tttatatcca aatgtatcag gtattcgctg cggtcgggag tgacgaattt tccgaacgga
     9301 ataaatccgc ccacaatggt caacagcgcc agaaacatca gcggataagt cattgagcgg
     9361 ggactttcgt gaggtttatg attcccgtga attttatatt ccttgcccca gaaaatatta
     9421 taataaagac ggaacatgta aaatgc
//
LOCUS       DAORKP010000091         9379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1003_length_9379_cov_7.156371, whole genome shotgun sequence.
ACCESSION   DAORKP010000091 DAORKP010000000
VERSION     DAORKP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_9379_cov_7.156371"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1208
                     /locus_tag="PLN75_06270"
     CDS             249..1208
                     /locus_tag="PLN75_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRG03755.1"
                     /translation="MMKESNYIRKYLTVFFFLLFPVIKISAEINKITEVFSTRNGQEL
                     KMDIYRSASSGVNPCIIHVFGGGFKEGARDQNYFSSYLKYFANAGFTVISIDYRLGMK
                     SVDAKGLKFVKALRQAIGFAVEDIYSATDYIIQNASELQIDTTRIILSGSSAGAVSVL
                     HADYEHCNNFDGDTILSDNFKFAGIISFAGAILSNKGVPSYKKSPSPTLFFHGTQDRL
                     VNYNKIQWFNLGLFGSKSITAQFRKYKYPYQFYSIENSGHEVAVNPMESRREEILYFI
                     QHLIFEKKPWMLDVYFNDINQKPSLMMSARDFYESKGSANQKK"
     gene            complement(1258..2247)
                     /locus_tag="PLN75_06275"
     CDS             complement(1258..2247)
                     /locus_tag="PLN75_06275"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRG03756.1"
                     /translation="MTQLHAVITGVGGYVPDYRLTNEELSRIVDTNDEWITTRVGIKE
                     RRILKGKNLGASYLGIKAVQDLFSKKDINPEEIEMVICSTSTPDHIFPSTASIIANKT
                     GITKAFCYDIQAACTGFLVAFDAASNYIQSGRYKKILIVSAEKMSASVDYTDRNTSPL
                     FGDGAGCVLVEPTTEEIGFVDSIIRNDNTGFKHLHMKAGGSAKPANHETVEKREHFVY
                     QEGTHVYKNAVWNMAEVSAELMNNNELTAEKLDWLIPHQANLRIIDAVVKRTDVPADK
                     TCINIHKYGNTSSASIPICIWEWENQFKKGDNIILTAFGAGFIWGAVYLKWGY"
     gene            complement(2327..2512)
                     /gene="rpmF"
                     /locus_tag="PLN75_06280"
     CDS             complement(2327..2512)
                     /gene="rpmF"
                     /locus_tag="PLN75_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRG03757.1"
                     /translation="MAHPKRKHSKQRTAKRRTHYKAALPTLAICSNCGASHIYHTVCP
                     ECGYYRGKLAIEKATAE"
     gene            complement(2528..3103)
                     /locus_tag="PLN75_06285"
     CDS             complement(2528..3103)
                     /locus_tag="PLN75_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRG03758.1"
                     /translation="MAKFDEYNIPLKGLKADSQDFEFNLDNQYFKKIDSPEVQQGNVK
                     AKVTVVKKTTLFELSFYLDGVIQIPCNRCLDDMNQPIQYKEKLQVKFGENFSEEGDIV
                     IVPESDGAINIAWFLYEFIVLNIPIKHVHAPGECNKTMVTKLKKHITRQKNADDDDVE
                     DIVFDDDDDISIEDTATDPRWDGLQNILENN"
     gene            complement(3287..4441)
                     /locus_tag="PLN75_06290"
     CDS             complement(3287..4441)
                     /locus_tag="PLN75_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRG03759.1"
                     /translation="MNRIYQTNQILKFVFIAVAMIIVVASTIFTNRLAEKLSVEETKK
                     IEVWAEATRQLILSDESTDMNFLLNIIEGNTTIPVIMTDENDNMLQHRNIKMPKSNEE
                     MFFSRKIARLKEVRPPIVVKLDADTRQFIYYDESFLLKQLYIFPYVQFGIIIIFLIIV
                     ILAFSSTKKAEQNQVWVGLSKETAHQLGTPISSLLAWTELLKLRHEDDKLINDMEKDV
                     RRLSIIAERFSKIGSKPDLKPTNLTEALNNAVGYMKNRSSNKVVITLNHPENELITAP
                     LNVPLFEWVIENLCKNAIDAMNGSGNIDITVKKSNTKEVIIDVKDTGKGMERKMHKAI
                     FTPGFTTKERGWGLGLSLAKRIIEEYHQGKIFVKQSEVGVGTVFRIVIPV"
     gene            4589..5479
                     /gene="era"
                     /locus_tag="PLN75_06295"
     CDS             4589..5479
                     /gene="era"
                     /locus_tag="PLN75_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRG03760.1"
                     /translation="MRSMHKSGFVNIVGNPNVGKSTLMNALVGERISIITSKAQTTRH
                     RILGIVNGEDFQIVYSDTPGVLKPNYRLQESMLSFSNSALTDADVLLYVTDIFDNHEK
                     NKEFVEKVNFNEAPVILIINKIDLTDQTKLEELVERWKSVLPKAEIFPISATEKFNID
                     PLFNRILSLLPVSPPFFEKDQLTDKPARFFVTEIIREKILLNYEKEIPYSVEVEVEQF
                     KEEENLIRINALIYVERDSQKGIIIGKGGKSLKKVGSEARKDIESFFEKKVFLELYVK
                     VEKDWRNKEIKLKGFGYKPE"
     gene            5609..5851
                     /locus_tag="PLN75_06300"
     CDS             5609..5851
                     /locus_tag="PLN75_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRG03761.1"
                     /translation="MKNSGGILAALLGGAVVGAALGILLAPDKGSETRAKIVDEFDTT
                     KEKVVEALKKRGINLTKNDLEDLVDDIKSEVQDVTA"
     gene            5857..6255
                     /locus_tag="PLN75_06305"
     CDS             5857..6255
                     /locus_tag="PLN75_06305"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRG03762.1"
                     /translation="MAVKTDNSNNGFQQLFADVKEYVKLQTDYWQVGLVEKLTKLLSK
                     LIIATISFVFIISMLFYLLFALAYALEPVLGFVASFVLIGVFFLILLLIIIVFRKQLI
                     INPILRIMVDVFYEDSNEKKPEDENHAATL"
     gene            6230..6526
                     /locus_tag="PLN75_06310"
     CDS             6230..6526
                     /locus_tag="PLN75_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03763.1"
                     /translation="MKTMQQLYEKKDLTFEDLSVIRKQLKDRIDLQQNQIQKSARRLI
                     PFSQNDGSLSLKNKAFSPLSLITTPIRKGKTLSLIEGIVIGYKLVRGIRRIIRK"
     gene            complement(6611..8437)
                     /locus_tag="PLN75_06315"
     CDS             complement(6611..8437)
                     /locus_tag="PLN75_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HRG03764.1"
                     /translation="MRKFILNTLTILLLIHVSIDIQAVPAYPYPVEVRQPDGTVLTLR
                     LRGDEFHHFLQTVDGYPVSKDEDGTYCYATLDIKGNLVSTKIKANNRSERNRSEVSFL
                     SGLEINELSTGEIRQMRSKRAQLSDNNPQKRIYPRTGSPRSLIILVNFADLSFVTPNP
                     QQAFTAMLNVKGYADNGGTGSARDYFIDNSIGKFSPDFDVVGPYTLPKGYAYYGKNDK
                     DGMDSIPFQMIVDACAAAHQAGINFSVYDADNDNIVDNIFVYFAGHNEAEWAGVNTVW
                     PHRWGIYPISLYRDGNYNGTVASVTFDGKRVEDYACTSELSGRTGSVMAGIGTFTHEF
                     GHVIGLADMYATNGATHHTLSDWNIMDGGAYLNKGRTPPAYNSFERFKLGYLSPKLLT
                     KPRNVLLRPLITDNEAFIVSPSDGHNLDGNNPVPTEFFMLENRQKTGWDKYLPGHGML
                     IYRINYNQNDWDYNQPNNDAANMAIDILEADNVANENTLSGDPFPGNSQVRSFQFETR
                     SGTKLENPLSDIAENFDGIISFYYGILPEKELRYSILDSNGSVLVFLSADEMEDIYVY
                     DTAGRLVQKTEANSTLIKVEGLHKNIIYIIKSGKRIAKIALR"
     gene            complement(8534..>9379)
                     /locus_tag="PLN75_06320"
     CDS             complement(8534..>9379)
                     /locus_tag="PLN75_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRG03765.1"
                     /translation="DKRYWERKRQEIRLKEKRLEETLARYETELEQTEKKRKEITLQA
                     KAEAENLLKAANAKIENTIRQIIEAKADKEKTQKARNELKEFSSPLSTIAIGTPKEGL
                     KKVQKSLTLKRKKLTNPTDIPPSGGWGAGVLVRIKGQNAVGTIIELQDKKAIVAFGQL
                     KSTVKLSQLEAVSNNQQKKENRKYENLGKTVTDDVRERKLNFKSEIDVRGMRGDEALQ
                     AVTYFVDDALMVGTASVRILHGTGTGILRQLIRQYLSTVHGVMKYHDEHVQFGGAGIT
                     VVEFE"
BASE COUNT         2703 a         1734 c         1825 g         3117 t
ORIGIN      
        1 tgccgttttt aatgaaagag caattacaga aatgtagttg ctcttttttt ttccatccgg
       61 taccattact gttaaaggga atagaaattt gagttccgaa tatatcggtt aatattcgta
      121 tttgtgtcat atttacaata taagtccttt acaagcaatt gtattgaata tattagtttt
      181 ccgaatatag tgaaagccgt atttttgaag attttattat atctttacag tctatttaat
      241 gtatttgaat gatgaaagaa tcaaattata taagaaaata tctgacggta tttttctttt
      301 tattatttcc tgtcattaaa atctcagcag aaatcaataa aataactgaa gttttctcaa
      361 ccaggaatgg ccaggaactt aagatggata tatatcgttc ggcaagttca ggagtaaatc
      421 cttgtattat tcatgtattt ggaggaggat ttaaagaggg agcgcgggat caaaattatt
      481 tttcatcgta tttgaaatat tttgcaaatg ccggatttac agtaatatcc atagattaca
      541 gattgggtat gaagtcagta gatgcaaaag gattgaagtt tgtaaaggca ttgaggcagg
      601 caattggctt tgctgtagag gatatatatt cagctactga ttacatcatt cagaatgcat
      661 cggaacttca gatagataca acccggatta tattaagtgg ttcaagtgca ggagctgtat
      721 cagtattaca tgcagattat gagcattgta acaattttga tggtgatacc atactatctg
      781 ataatttcaa atttgccggt attatttctt ttgctggtgc tattttgagt aataagggag
      841 taccatctta caaaaaaagt ccatcgccta cactcttttt tcacggaact caggatagat
      901 tggtgaatta caataaaata caatggttta atttgggctt atttggttca aaatctatta
      961 cagcacaatt tagaaaatat aaatatccat atcagtttta tagtatagaa aatagtggac
     1021 atgaagttgc tgtaaatcca atggaatcca gaagagaaga aattctttac ttcattcaac
     1081 acctgatttt tgaaaagaaa ccttggatgc tggatgttta ctttaatgat attaatcaga
     1141 aaccgtcact catgatgagt gccagagact tttatgaatc gaaaggatct gctaatcaaa
     1201 aaaaataaag ccacctaacg atggctttat tgaaggatgt atagagtata taaattatca
     1261 gtatccccat ttgagataaa ctgctcccca gataaatccg gctccaaagg ctgtgagaat
     1321 gatgttatct ccttttttaa attgattttc ccattcccaa atacaaattg gtatgctggc
     1381 tgatgatgta tttccgtatt tatgaatgtt gatacaagtc ttatcggcgg gaacatctgt
     1441 acgttttact actgcatcaa ttattctcag attggcctgg tgaggtatga gccagtctaa
     1501 cttttcagct gtcaactcgt tattattcat cagctcggct gagacttccg ccatattcca
     1561 aacggcattt ttataaacgt gagttccttc ctgataaaca aagtgctccc gtttttcaac
     1621 tgtttcatgg ttagccggtt ttgccgaacc gccagctttc atatgcaagt gcttaaagcc
     1681 tgtattgtca ttacgaataa ttgaatcaac gaatccgatt tcttccgttg tgggctctac
     1741 aagcacgcag cctgctccat ctccaaataa cggactggta ttacgatcgg tatagtcaac
     1801 cgatgcagac attttttcag cagatacaat aagaattttt ttatatcttc ctgattgaat
     1861 gtaatttgat gcagcgtcaa atgcaaccag aaaaccggta caagcagctt gaatgtcata
     1921 acagaaggct ttcgttatgc ctgttttatt tgctattatg gatgctgtgg atgggaaaat
     1981 atgatcaggt gtggatgtgg aacaaattac catttcaatt tcttcaggat ttatgtcctt
     2041 ttttgaaaat aaatcctgta cagcttttat ccccagatac gatgctccca gatttttgcc
     2101 cttgagaata cgtctttctt ttattcccac gcgggtggta atccactcgt cgttggtatc
     2161 cactattcga cttaattctt catttgtgag cctgtaatca ggtacataac cgcctacgcc
     2221 tgtaattacg gcatgtaatt gcgtcatgaa attttagttt tttaaaataa gaaatcaagt
     2281 aaacatttta atatttgtgt ttacttgatt taagtgtata gaaagattat tctgctgtgg
     2341 ctttttcgat agccagttta cctctgtaat aaccacattc aggacataca gtatgataaa
     2401 tgtgacttgc accacagttt gaacagattg ctaatgttgg caaagctgct ttataatgtg
     2461 tccgtctttt agcggttctt tgttttgagt gttttcgttt aggatgtgcc attttagtat
     2521 tcttttttta attgttttct aatatgtttt gcaggccgtc ccatcgcggg tcagttgcag
     2581 tatcttctat tgaaatatcg tcatcatcat caaatacaat gtcttcaaca tcatcgtcat
     2641 ctgcattttt ttgtcttgtg atgtgttttt ttagcttggt taccatcgtt ttattacatt
     2701 cgccgggtgc atgtacatgt ttgatcggaa tgttcagcac gataaattcg tataaaaacc
     2761 aagctatatt gattgctccg tctgattccg gaactatcac aatatcgcct tcttctgaaa
     2821 aattttcacc aaatttaacc tgaagttttt ctttgtactg aataggttga ttcatatcgt
     2881 ccaggcatct gttgcatggg atttgaatga caccgtccag gtaaaaggaa agttcaaaca
     2941 gtgttgtttt ttttacaaca gtgacttttg cttttacatt tccttgctga acttcaggac
     3001 tgtcaatttt cttgaaatat tgattatcca gattgaattc aaaatcctgg ctatctgcct
     3061 tcaatccttt caaaggaatg ttatattcgt caaatttagc cataatgaat tatctaaaaa
     3121 ccatgcaaag gtacaaaatt tagcttgaaa ataaaagttt taatacaaaa ttatttgcaa
     3181 tataactgat ttgtatgtct gtgagattat ggtgttttct agtgttaaca tcacacggga
     3241 ttaatgtttt tattaacaga ccatcaaatg cttgtcaaac aggttattat acgggaatta
     3301 ctattctgaa aaccgtacca acacctactt cggattgttt aacgaaaatt tttccctgat
     3361 gatattcttc tatgattctt tttgcaagag aaagacccaa gccccaccca cgctctttag
     3421 tggtgaatcc cggagtgaat atggctttat gcattttgcg ttccatccct tttccggtat
     3481 cttttacatc aattatcact tctttggtgt tggatttctt aactgtaatg tcaatattac
     3541 cgcttccatt catggcgtcg atggcatttt tacaaagatt ttcaatcacc cattcgaata
     3601 acggaacatt caatggcgct gtaattaatt cattttcagg atgattgagt gttataacca
     3661 ctttgtttga gcttctgttt ttcatgtatc ccacggcatt gttcaacgct tctgtcagat
     3721 tggttggttt caggtcgggt ttggaaccaa tttttgagaa tcgctcggca atgatgctca
     3781 atcgcctgac atccttttcc atgtcgttaa tcaacttgtc gtcttcgtgg cgcagtttga
     3841 ggagctcggt ccaggcgagc aacgatgaga tgggtgtgcc cagctgatga gcggtttctt
     3901 ttgaaagccc tacccaaacc tggttttgtt ccgctttttt tgtacttgaa aatgcaagta
     3961 ttacgataat gagaaatatg attataatac cgaactgcac atacgggaaa atatataact
     4021 gcttgagtaa gaatgactca tcgtagtaga taaattgacg ggtgtcagca tcgagtttta
     4081 ccactattgg cggacgtact tctttcagtc gggctatttt tctgctgaaa aacatttctt
     4141 cattgctttt cggcattttt atatttcggt gttgtaacat gttgtcgttt tcatctgtca
     4201 tgattaccgg aatagtagtg ttgccctcta taatatttag cagaaaattc atgtcggtag
     4261 attcgtcgga cagtatcaac tggcgtgtag cttcggccca gacctctatt ttcttggttt
     4321 cctccaccga tagcttttca gcaagcctgt ttgtgaatat agtagatgca acgactatta
     4381 tcattgctac tgcaataaat acaaacttaa ggatctgatt ggtctgatag attctattca
     4441 taagtaactt aaagaataaa cgctgagtat tattaacgac aaaattaatg aaaattgtct
     4501 gatgtgaaaa aatatcttca tattccggtt ccattcatcc ttcaaagccc tcgaaattgt
     4561 tagtgaaaat atgtatcttt gtaaaaaaat gagaagtatg cataagtcag gttttgtaaa
     4621 tattgtaggg aatcccaatg taggaaaatc aacattaatg aacgcattgg tgggagagcg
     4681 catttcaatc attacatcca aagctcagac tacacgccac aggattttgg gaattgtgaa
     4741 tggtgaagat tttcagatag tatactccga tactcccgga gtgctcaaac caaactatcg
     4801 gcttcaggaa tccatgctga gtttttcgaa ttctgcactt acagatgccg atgtactgct
     4861 gtatgtgacc gatatatttg acaatcatga aaaaaacaaa gaatttgtag agaaggtaaa
     4921 ttttaacgag gcacctgtaa ttctgataat caacaagatt gacttaacag atcaaacaaa
     4981 actggaagaa ctggtagaaa gatggaaatc agtattgccg aaagcagaaa tatttccgat
     5041 atcggctact gagaaattca acatcgatcc tcttttcaat cgcattttat ctttattgcc
     5101 cgtttcgcca ccttttttcg agaaagatca gcttaccgat aagccggcaa ggttttttgt
     5161 aacagaaata atcagggaaa agatactgct gaattatgaa aaagaaattc cctactcggt
     5221 ggaagtggaa gtggaacaat ttaaagaaga agaaaatttg atccgaatca atgcactgat
     5281 ttatgtagaa agggattcgc agaagggaat tataatcgga aaaggaggaa aatcacttaa
     5341 gaaagtgggt tctgaagccc gaaaggatat cgaaagtttt tttgagaaaa aagtttttct
     5401 cgaattgtat gtaaaagttg aaaaggactg gcgaaataaa gaaatcaaac tcaagggatt
     5461 cggatacaaa cctgagtgaa ttatctgagt ttttaaccct gcgcggatat aatttaccac
     5521 caatttttaa tattttggca aactttttga ttaaaagcgt gttatagtta tattaaagta
     5581 agttattaac aaattaattt ttactattat gaaaaattca ggtggcattt tagcagccct
     5641 tttgggcggt gcagtagttg gagcagcatt aggcattctt ttagctccgg ataaaggctc
     5701 ggaaacaaga gcaaaaatcg tggatgaatt cgatacaaca aaagaaaaag tggttgaagc
     5761 tttgaaaaaa agaggcataa atcttactaa aaatgacctc gaagatctgg tagatgatat
     5821 aaaatcggaa gtacaggacg taacagcgta aaaaacatgg ctgtcaaaac agataactcg
     5881 aataacggtt ttcaacaact ttttgctgat gtaaaagagt atgtgaagtt acaaactgat
     5941 tactggcagg taggattggt tgagaaattg actaagttac tttcaaaatt aattattgcc
     6001 accatttcgt ttgtgtttat catcagtatg ctgttttatc tgttgtttgc tcttgcttat
     6061 gccttagagc cggttttggg atttgtagca agctttgtgt taatcggagt tttttttctg
     6121 attttactgc tgatcattat agtttttcgc aaacaattga ttatcaatcc aatactcaga
     6181 attatggtag atgtttttta tgaagattcc aacgaaaaaa aaccggaaga tgaaaaccat
     6241 gcagcaactt tatgaaaaaa aagacctgac ttttgaggac ttatccgtta tcagaaaaca
     6301 actcaaagac aggattgatt tacagcaaaa tcaaattcag aagtctgcca ggcgtttgat
     6361 tccattttcg caaaacgatg gaagtctgtc attgaaaaac aaagcctttt ccccactttc
     6421 gctgattact acgcccattc gaaaaggtaa aactctttcg ctcatcgaag gaatagtaat
     6481 cggttacaaa ctcgtaagag gcatacgaag gattattcgt aaatgatatt caaacactta
     6541 cattaaaaaa ggccggttaa aaaaaccggt cttttttatg atggtaagat aaaatcaggt
     6601 gaaaacgata ttatctcaat gcaattttgg caatcctttt tcctgattta ataatgtaaa
     6661 taatattttt gtgtaatcct tcaactttta tcaaagtgga gttcgcttca gttttctgaa
     6721 ccaatcttcc tgccgtatca tacacatata tatcttccat ttcgtcggca ctaagaaaca
     6781 ccaatacact tccgttgcta tccaggatgg aatatctcaa ttccttttcg ggtaaaattc
     6841 cgtagtaaaa agaaataatt ccatcaaaat tttcggcaat gtcactcaat ggattttcaa
     6901 gcttagtacc gctgcgggtt tcaaactgga aacttctgac ctgcgaattg ccgggaaacg
     6961 gatcgccgga aagtgtattc tcattggcca cattatcggc ttccaggatg tcgatagcca
     7021 tattagcagc atcattgttt ggttggttgt agtcccagtc attttggttg taattgattc
     7081 ggtaaataag catcccatga ccgggtaaat atttgtccca accggttttc tgacggtttt
     7141 cgagcatgaa aaattctgtg ggaaccggat tgtttccgtc gaggttatgc ccgtcggaag
     7201 gcgaaacgat gaacgcttca ttatcggtaa tcaacggacg caataaaaca tttctgggtt
     7261 ttgtcagcaa ttttggagat aaataaccca atttaaagcg ttcaaacgaa ttgtatgcag
     7321 gaggtgttcg gcctttgttc agataggctc ctccgtccat gatattccag tcggacaagg
     7381 tatgatgagt agcgccattt gtggcgtaca tatcggcgag tccgataacg tgtccgaatt
     7441 catgggtgaa agtgccaata ccggccatta cagagcctgt tcttccactt aactccgatg
     7501 tacaggcata atcttccact cttttcccgt cgaaagtgac tgatgccacg gtaccgttgt
     7561 aattgccatc tctgtagagg cttatggggt atattcccca gcgatgtggc cacacggtat
     7621 ttactcccgc ccattcagct tcgttgtgtc cggcaaagta aacgaatata ttgtcaacga
     7681 tgttatcgtt atctgcatcg tacaccgaaa agttaattcc tgcctgatga gctgctgcac
     7741 atgcatctac tatcatctga aacggaatgc tgtccattcc gtccttatca tttttaccgt
     7801 agtatgcgta gccttttggc aatgtataag gtcctaccac atcaaaatcg ggactgaatt
     7861 ttccaatgga attatctata aaataatcgc gtgcagaacc tgtacctcca ttgtcagcgt
     7921 atccttttac attgagcatg gctgtaaagg cttgttgtgg gttgggggtg acaaaagata
     7981 gatctgcaaa gttgaccaga ataatcagtg aacggggtga ccctgttcgg ggataaattc
     8041 ttttttgtgg gttgttatca gacaattgag ctctttttga acgcatttgc ctgatctctc
     8101 cggtgctaag ttcatttatt tccaggcccg aaagaaaaga tacttcggaa cgatttcgct
     8161 cgcttctgtt gttggccttt atttttgtgc ttaccagatt ccctttgata tcaagggtag
     8221 cataacagta ggtaccatct tcgtcttttg aaaccggata accatctacc gtttgtagaa
     8281 aatgatgaaa ttcatctccc cggagtcgga gtgtaagtac agtaccatcg ggctgacgta
     8341 cttcaaccgg ataaggatat gccggaactg cctgaatgtc aatactgaca tggataagca
     8401 gaaggattgt taaagtattt agaatgaatt tcctcatttt atggatattt ttaatttaca
     8461 aatttatctt ttttcttcag aattccaccg tttctgttga aaattaacct cttttctgtc
     8521 atcagctgct acattattca aattctacca ccgtaatacc cgcaccgcca aactgaacat
     8581 gttcgtcgtg gtatttcatc actccatgta ccgtggagag gtactgccgg ataagttgac
     8641 gcagaatgcc ggtgcctgtt ccgtgaagta tgcgaacgct cgcagtgcct accatcaggg
     8701 catcgtcaac aaaataagtc actgcctgca aagcttcgtc gccacgcatg ccgcgtacgt
     8761 caatttccga tttgaaatta agttttcgtt cccgtacatc atcagtaacg gtttttccta
     8821 ggttttcgta cttgcggttt tcctttttct gctgattatt gctaacggct tcaagttggc
     8881 ttagttttac ggtagatttt agttgaccaa atgctacgat tgccttttta tcctgaagct
     8941 caataattgt gccgactgca ttttggcctt tgatacggac aagaactccg gccccccaac
     9001 cccccgaagg ggggatgtca gtggggttag tcagcttttt tcgttttaaa gtaagacttt
     9061 tttgcacttt ttttaagccc tctttcggag taccgatagc tattgtggac aggggacttg
     9121 agaattcttt taactcattc cttgctttct gcgttttttc tttatctgcc ttcgcttcaa
     9181 taatctgccg aatggtattt tcaatctttg cattggcggc tttcagcaga ttttcagctt
     9241 ccgcttttgc ttgcagggtt atttcctttc tttttttctc ggtttgttcc agttcggttt
     9301 cgtatctggc aagcgtttcc tcgaggcgtt tttctttgag tcggatttcc tgtcgcttgc
     9361 gttcccagta gcgtttatc
//
LOCUS       DAORKP010000092         9010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1099_length_9010_cov_9.420771, whole genome shotgun sequence.
ACCESSION   DAORKP010000092 DAORKP010000000
VERSION     DAORKP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_9010_cov_9.420771"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLN75_06325"
     CDS             <1..449
                     /locus_tag="PLN75_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRG03766.1"
                     /translation="PEVSLVAILDADKEGFLRNHRSLTQTAGRAARNLNGKVIMYADK
                     TTASMQKTIDETNRRREKQLAYNAKYGITPTQITKGERSSLSKMEPNAYIEPETPNIL
                     AADPVVKYMSKSALEKAIAKAKKSMQEAAKKLDFLEAAQYRDEMIK"
     gene            complement(543..989)
                     /gene="rplI"
                     /locus_tag="PLN75_06330"
     CDS             complement(543..989)
                     /gene="rplI"
                     /locus_tag="PLN75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRG03767.1"
                     /translation="MEIILKEDVINLGYKGDIVKVKDGYGRNFLIPTKKAVIASESAK
                     KMLAEDNKQRAHKFEQIKNNAVELSEKIAALGSLTIGAKTSSTGKIFGAVGPIQIAEA
                     LEKAGVTVDRKVIAIKEPIKEVGSYTASVKLHKEVSAEISFEVVSE"
     gene            complement(1009..1272)
                     /gene="rpsR"
                     /locus_tag="PLN75_06335"
     CDS             complement(1009..1272)
                     /gene="rpsR"
                     /locus_tag="PLN75_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRG03768.1"
                     /translation="MAQGEIRYLNPPTVDVKKKKYCRFKKSGIKYIDYKDADFLKKFL
                     NEQGKILPRRITGTSLKYQRKVAQAVKRARHLSLLPYVTDMMK"
     gene            complement(1277..1681)
                     /gene="rpsF"
                     /locus_tag="PLN75_06340"
     CDS             complement(1277..1681)
                     /gene="rpsF"
                     /locus_tag="PLN75_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRG03769.1"
                     /translation="MLNHYEAVFILTPVLSDVQMKEAVDKFKAVITDNGGTITNEENW
                     GMRKLAYPIQKKSTGFYSLLQFDVEPAVIATLETQFRRDERVLRFLTFRLDKYAYEYA
                     EKRKNVKSQPKEVKEEKVTRKEVKEEIKEVEE"
     gene            2000..3892
                     /locus_tag="PLN75_06345"
     CDS             2000..3892
                     /locus_tag="PLN75_06345"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HRG03770.1"
                     /translation="MKKIFLLFGLFLFTGSIIAQSPDKKKPVKDKFVKSWHIDEQFAI
                     ADTVPIDTARLNFQENNIIERFSTDNSYNANYGSPIQSKIYMRRPQGTDFMFEDAYKP
                     YLLDLSSALFYDANFPYTNLTYLTGGPVIGKEEQVKFLYTGSPSKKANFGLNLDYLNS
                     KGRFANQAAQRFTGGIFGRYSGKHYSAYGLAAVNNHFNHENGGLSNLELLNNPNVDVQ
                     TKDMPTYIHGYSVFRKNTFFYNHNYSVGINREVKISEDSTAYEYVPVTRFGHMIKFEE
                     MKKRYYEPQLEKSFYKNSYDSINNTNDTAAVRTLTNLLSINLAEEFNRWMKFGLTGYV
                     ENEVQQFTFFRDSLLQKDTRSNTRVGGVLSKSRGTNLRYSVLGDIYLIGYKLGEFRLE
                     GKAEGSFRIFKEDVLLAANAFVRNEEPSYFIQNYYSNHFRWENNFTKMYKTHVGGTFS
                     LPKRKTRLNVAVENLTRLIYFDENAMPVQHDGNVQVLSADLKQDVSFGPFTLENNAVY
                     QVSSNQDVLPLPMLTLYHNFYYHDKWFVDLYPQFGVSVRYHTNYFAPSYMPATGQFYN
                     QKVVEIGNYPVLSAYANFHLKKARFFFEYNNLARYFMNGWGFHMPNYPINPPMFKMGL
                     SWNFYN"
     gene            3981..4721
                     /locus_tag="PLN75_06350"
     CDS             3981..4721
                     /locus_tag="PLN75_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRG03771.1"
                     /translation="MNRSRAHSAGTVTLFETGKLRVATEKSRVGFALGEKTALGFNYE
                     IVKAFAHSMDLELEISLVNNMDSAITGLHQNKYDLVACNIPNTGEMQKKVNLSVPLLN
                     SRQMLIQRTGNDSVAADTLVTDHRMLHNQKIYIAGGSPFRFRLENLSGEIADTIHITE
                     VESATTEKLVKAVSEGKIKYTICDEQQARKLSAQYSNIDFSVPVGFNQHFCWAVDKKS
                     LRLLHEINEFLNDFLQSDDYWNIYRKYY"
     gene            4778..5695
                     /gene="metA"
                     /locus_tag="PLN75_06355"
     CDS             4778..5695
                     /gene="metA"
                     /locus_tag="PLN75_06355"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRG03772.1"
                     /translation="MPLNIPKMLPAVETLRKENIFVMDTDRAQSQEIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEIDFLQLKSHTPKNTPIEHMMAFYRNFDEIRKSNYDG
                     MIITGAPVELLDFEQVKYWKEVTDIFEWAHRHVTSTLYICWAAQAGLYHFYKVPKYPL
                     AEKMFGVFEHKTNNSHNPIFRGFDDVFYVPHSRHTEIRREDILKIPELTLLSESDRSG
                     VYMVMARNGREFFITGHSEYSCYTLDTEYKRDLKKGLPIKMPENYYLDDNPKNEPVVR
                     WRSHANLLFTNWLNYFVYQETPYDLANIR"
     gene            5703..7523
                     /locus_tag="PLN75_06360"
     CDS             5703..7523
                     /locus_tag="PLN75_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRG03773.1"
                     /translation="MRTIELLSPAKNLECGLAAVNHGADAVYIGAPMFSARAAAGNSL
                     ADIEQLISYAHRYRSKVLVALNTVLTDSQLTEAQKLITQIYETGADALIVQDLGILQL
                     DLPPIELHASTQTDNRTVEKVKFLQDAGFSRVVLARELTLNQIRQISSQTDVELECFV
                     HGALCVSYSGQCYISQAMTGRSANRGECAQYCRLPYQLTDSNGQVLVKNKHLLSLKDL
                     DLSDHLEELTDAGVTSFKIEGRLKDADYVKNITAYYRKKLDAILEKRSDVKKSSQGTT
                     TFFFEPNPDKSFRRSATDYFLNGRHRDISQPETPKSVGEEIGIITYVSSQYIEIKTEK
                     DIHNGDGLSFFAKDGTLSGFRVNRVEGKRIFPAEMPVTEPNILLYRNQDHEFDKILAG
                     KTSERKIGVDIQFSETSDGFSIQITDEEGISAGCEFVSEKQPANNAERALQTIESQLT
                     KLGNTIYTARNVDISVSAPRFLPASALNEWRRKTIELLDEKRLKAYITRKRKPANTEA
                     VFPEKKLTYLGNVTNERAREFYLQHGVEEIMPGFEVIAEPDVPLMFCKHCIKYSLGWC
                     PKEGYRATFREPLYITYKNQQFQLEFDCRKCEMMVKKRKD"
     gene            complement(7589..>9010)
                     /locus_tag="PLN75_06365"
     CDS             complement(7589..>9010)
                     /locus_tag="PLN75_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRG03774.1"
                     /translation="KFNAETIQDAQINLKYFKNQAYTLEAKSVPGYTFKQWEIPAGSN
                     SQMLIANGSIWKYWDGNAMPASNWFSPGYSDSGWKTGIASLGYGGLGHTTVIGYGSDA
                     NNKYPTAYFRKTVNITDLASKDNFEISTFVDDGAAVYVNGTEVGRVNMPAGTLTFNTY
                     STTYNNGVTGTFTVPKNLLTEGNNVIAVEVHQTGANSSDLFFNLQFTCTISGESQVIL
                     SPVFASTLTGTFNIRAIYEKNSGEDPDKDLQVVINEIVASNTIIADEFGDKDDYIEIF
                     NNSNREVNIAGWYISDTPANPTLYQIPATNAALTTIPAKGRLVIRADDESSQGVLHAS
                     FKLSKDGETVTLSRLNYLGQLTEMDRVTFPYMEQNMSYSRIPDGSSTWLVTSPTCNLA
                     NSNQTSTDDIRLTARMYPTLADNYLIVENAQNRLIRVIDLTGKVLMQQTSNDEKIRLE
                     LSNLKPGVYILTAGNEKFRFIKK"
BASE COUNT         2547 a         1965 c         1981 g         2517 t
ORIGIN      
        1 tgcccgaagt ttcgctggtg gctatcctcg atgccgataa ggaaggattt ctgcgaaatc
       61 accgttcgct cacacagacc gccggacgtg cggcccgaaa tctgaacggg aaagtaatca
      121 tgtatgccga taaaacgact gcgagcatgc agaaaaccat cgatgaaacc aaccggcgac
      181 gcgaaaaaca gcttgcctac aacgccaaat acggcattac acccactcag ataacgaaag
      241 gtgaacgcag ttcactcagc aaaatggagc cgaatgctta cattgagccc gaaacaccca
      301 atattctggc tgccgatccc gtagtgaagt acatgtcaaa atccgcgctc gaaaaagcta
      361 ttgccaaagc caaaaaatcg atgcaggaag cagctaaaaa actcgatttt ctggaagccg
      421 cacaatatcg ggatgaaatg attaagtagg aggaattgat gaaagggaaa gaatgaaaaa
      481 aaccgaaaca ctcaaatgaa tgagtatttc ggtttttcgc gggattttga atcgaaagga
      541 acttattcag aaaccacttc gaatgagatt tcagcagaaa cttccttgtg aagttttaca
      601 cttgctgtat atgaacccac ttctttgatc ggttctttga tggcaattac ttttctatcc
      661 acagttacac cggctttttc aagagcttct gctatttgga ttggacctac ggcaccaaat
      721 attttgcctg ttgagctggt ttttgcacca atggtaaggc tgccaagcgc tgcaattttt
      781 tcgctgagtt caacggcgtt atttttgatc tgttcaaatt tatgtgcgcg ttgtttgttg
      841 tcttctgcca acattttctt agcagattca ctggcgataa cggctttttt ggtcggaatc
      901 aaaaagttac gtccgtaacc gtctttcact tttacgatgt cgcctttgta acccagattg
      961 attacatctt cttttaatat aatttccata atcttttctc cttttttatt atttcatcat
     1021 atcggttacg taaggaagca atgagaggtg gcgtgctctt ttaactgcct gagccacttt
     1081 tcgctgatat ttaagcgaag ttccggtgat tcggcgggga agtatttttc cctgctcgtt
     1141 cagaaatttt ttcagaaagt cggcatcttt ataatcgatg tatttgatac cgcttttttt
     1201 gaaacgacaa tattttttct tttttacatc cactgtcggt ggattcaagt atctgatttc
     1261 tccttgtgcc atgattttat tcctccactt ctttaatttc ttctttaact tctttcctgg
     1321 ttactttttc ctctttcact tctttgggtt gtgattttac gttttttctt ttttcggcgt
     1381 actcgtaagc gtacttgtcg agacggaaag ttaagaaacg aagtacacgt tcgtcgcggc
     1441 ggaactgggt ttccagtgtg gcgatcacgg cgggttcaac atcgaactgc aacagggagt
     1501 aaaatcctgt cgattttttt tgaatagggt aagccagctt gcgcatgccc cagttttctt
     1561 catttgtgat agttcctccg ttgtctgtga tgacagcttt gaacttatct accgcttcct
     1621 tcatctgaac atcagacaaa acgggagtta aaatgaaaac ggcttcataa tgatttaaca
     1681 tactgttttt tacctgttta atggtttgta aatggtttta ataaagtggg tgcaaagata
     1741 tgaataattc tttgattgac aaaacaatcc cgggaaaaag ttgatgggtt gtttgattgt
     1801 ttgattgttt gggtgtttgg tttacaggga ggttgttgag cggagtctgc tgtatcccat
     1861 tcaatgattt tttttcttgt taataaatta aaatctccat ccaaatattg tcaaactacc
     1921 aaactttcaa agcggcaaat actcaatttt caaactcatt tcgtatcttt gcgactttat
     1981 aaacgcacag caattcctga tgaagaaaat ttttctgctt tttggtctgt ttctttttac
     2041 cgggtcaatt atagcccaga gcccggacaa aaaaaaaccg gtaaaagata aatttgtgaa
     2101 atcgtggcat atcgatgagc agtttgccat tgccgatact gtacctattg atactgcacg
     2161 tcttaatttt caggaaaaca acatcatcga aagattcagc accgacaatt cctataatgc
     2221 caactatgga tcgcccatac agtcgaaaat atacatgcga cgtccgcagg gaaccgattt
     2281 catgtttgaa gacgcctaca aaccctacct gctggatctt tcctcggcgt tattttacga
     2341 tgccaatttt ccttatacca accttacgta tctcaccggt ggtcctgtta tcggtaaaga
     2401 agaacaggtg aaatttctct acacaggcag tccgtcaaaa aaagccaatt tcggactgaa
     2461 cctcgattac ctcaattcaa aaggaagatt tgccaatcag gctgctcagc ggtttacagg
     2521 aggtattttc ggccggtatt cgggcaaaca ttattcggcc tacgggttgg ctgccgtcaa
     2581 caatcatttc aatcatgaaa atggcggact gagcaacctc gagctgctga ataatccaaa
     2641 tgtggatgtt cagacaaaag atatgcccac atatatacac ggttattctg tttttcggaa
     2701 aaataccttc ttttacaacc acaattacag tgtggggatt aatcgcgaag tgaaaatcag
     2761 cgaagattcc acagcttacg aatacgttcc cgtcacccga ttcggacaca tgatcaagtt
     2821 tgaagagatg aaaaaacggt attacgaacc tcagctcgag aaatcattct acaaaaattc
     2881 gtacgattcc atcaacaata cgaatgatac agccgcagta cgtacgctta caaacctgct
     2941 atccataaat ctggccgaag aattcaaccg gtggatgaag ttcggactca ccggctacgt
     3001 ggaaaacgaa gtgcaacagt ttacgttttt cagggatagt cttctgcaga aagacaccag
     3061 atccaatacc cgcgtaggag gtgttctttc taaatcacgg ggcacaaatc tgaggtattc
     3121 ggtgttgggc gatatttatc tgataggcta taaactcggc gaattcagac tggaaggcaa
     3181 agccgaaggt tctttccgta ttttcaaaga agatgtactg cttgccgcca acgcttttgt
     3241 gcgcaacgaa gaaccttcgt attttattca gaattattac tccaatcatt tcaggtggga
     3301 aaataatttt acaaaaatgt ataaaacgca tgtgggtggt actttttcgt tgcccaaacg
     3361 aaaaacacgg ctcaacgtag ccgtggaaaa cctcaccagg ctgatatatt tcgatgaaaa
     3421 tgccatgccc gtgcagcacg acggcaatgt acaggtactc tctgccgatt tgaagcagga
     3481 cgtaagtttt ggcccgttca cgcttgaaaa caacgctgtg tatcaggtca gctccaacca
     3541 ggatgtgctg ccgctgccca tgctcacgtt gtatcataac ttttactatc acgataaatg
     3601 gtttgttgac ctgtatccgc agtttggtgt gagcgtaagg tatcatacca actattttgc
     3661 accgtcgtac atgcctgcca ccggccagtt ttacaaccag aaagtggtcg aaattggaaa
     3721 ttatccggtg ctcagcgcgt atgccaattt tcacctgaaa aaagcacgct ttttcttcga
     3781 atacaataac ttagcacgtt attttatgaa tgggtggggc tttcacatgc caaattatcc
     3841 gattaatccg cccatgttca aaatgggcct ttcgtggaat ttttacaatt gattctttta
     3901 tgaacaatcg gaagtgtgga ataattacag ggctgagtgg cctccttctt ttggttggga
     3961 tattaatgct cagcgtttta atgaacaggt cacgtgcaca ctctgccgga acagtcacct
     4021 tgtttgaaac aggaaaactg cgtgtagcta ccgaaaaaag cagggtcgga tttgcacttg
     4081 gcgaaaaaac ggccctggga tttaattatg aaatagtgaa agcttttgct cactccatgg
     4141 acctggaact cgaaatcagt ctggtgaaca acatggattc ggccataacg ggtttacatc
     4201 agaacaaata tgatctggta gcctgcaaca taccgaatac gggtgaaatg caaaaaaagg
     4261 taaatctcag cgttccgcta ctgaattcgc gacagatgct tattcagcgg acagggaatg
     4321 atagtgtagc tgccgatacg ctggtcactg accatagaat gctgcataat cagaaaattt
     4381 acatagccgg aggctcacct ttcagatttc gccttgagaa tttatccggt gaaattgctg
     4441 ataccattca tattacagaa gtggagtcgg ccacaacaga gaaactggta aaagccgttt
     4501 cggaaggaaa aataaaatat accatctgcg acgaacagca ggcccgcaag ctgagtgcac
     4561 agtattcaaa tatcgatttc tcggttccgg ttggatttaa tcagcatttt tgctgggcag
     4621 tggataagaa atcgctcaga ctgcttcacg aaataaatga atttctgaac gattttttac
     4681 aatcagacga ttactggaac atttaccgaa agtattattg attcgttcag taataagcat
     4741 taaaataacc ccagactcta tactataaac tgttgatatg ccattaaata tccccaaaat
     4801 gcttccggcc gtcgaaacac tgcgcaaaga aaatatcttc gtgatggata ccgacagggc
     4861 gcaaagccag gaaatcaggc cgcttaagat agtgattctc aacctgatgc ccctgaaaat
     4921 taccaccgaa accgatttga tccgcttgct ctccaattcg ccgctacaga ttgaaattga
     4981 ttttctgcag ctcaaaagcc atactcccaa aaatactccc attgagcaca tgatggcttt
     5041 ctacaggaat tttgatgaaa tccgtaaaag caattacgat gggatgataa tcaccggagc
     5101 gccggtggaa ctgcttgatt ttgagcaggt aaaatactgg aaagaagtga ctgatatctt
     5161 tgaatgggca catcgtcatg ttacttctac gctatatatc tgctgggctg cccaggccgg
     5221 gttgtatcat ttctacaaag ttcctaaata cccgttagcc gaaaaaatgt tcggagtatt
     5281 cgaacataaa accaacaatt cacacaatcc tatttttcgg ggtttcgacg atgtgtttta
     5341 cgtacctcac agccgtcata ccgaaattcg gcgcgaagac atactgaaaa tacctgaact
     5401 gactttgctg tcagaatccg accgttcggg agtatatatg gtcatggcac gcaacggacg
     5461 cgaatttttc attacaggcc actccgagta ttcctgctat acgctcgaca ccgaatacaa
     5521 gcgcgatttg aaaaaaggcc tgcccatcaa aatgcccgaa aactattacc tggatgataa
     5581 tccgaaaaat gaacccgtag tgcgctggag aagtcacgcc aatctgctgt ttaccaactg
     5641 gctgaactat ttcgtatatc aggaaacgcc ctacgatttg gctaatatcc ggtaaccgaa
     5701 tcatgcgcac catcgaactt ctatcgcccg ccaaaaacct ggaatgtggt ttggctgctg
     5761 tcaatcacgg tgcagacgct gtttatatcg gtgcaccgat gttcagtgcc cgcgctgcag
     5821 ccggaaattc actggctgat attgaacagc ttatcagtta tgcccaccgc taccgcagca
     5881 aagtactagt ggcattgaat accgtgctga ctgacagtca gctaacagaa gcccaaaaat
     5941 taatcactca gatttatgaa acaggagctg atgctttgat tgtgcaggac ctgggcatac
     6001 ttcaactgga tttgcctcca atcgaactcc acgccagcac gcagaccgat aaccggactg
     6061 ttgaaaaggt gaaatttcta caggatgcag gtttctcaag ggtggtgctg gcaagggaat
     6121 tgacgctgaa ccagatacga caaatcagca gtcagaccga tgtcgaactc gaatgttttg
     6181 tgcatggcgc cttgtgtgta agctacagcg ggcaatgcta catcagccag gcaatgaccg
     6241 gacgcagtgc caatcgcggt gaatgcgctc aatattgcag gcttccgtat cagcttaccg
     6301 acagcaacgg acaggtactc gtgaaaaaca aacacttgct ttcgctcaaa gacctcgatt
     6361 tatccgacca cctggaggaa ttgaccgatg ccggggtaac ttcgttcaaa attgaaggca
     6421 gactgaagga tgccgattat gtgaaaaata ttacggctta ctacagaaag aaactggatg
     6481 caattctgga gaaaaggagc gacgtaaaaa aatcatcaca aggaacaaca actttctttt
     6541 ttgaaccaaa tccggacaaa agtttcagaa gaagtgccac cgattatttc ctcaacggac
     6601 gccatcggga tatcagtcag cccgaaacac cgaaatccgt tggtgaggaa atcggaatca
     6661 tcacgtatgt ttcatctcag tatatcgaaa taaaaacaga aaaggatatt cacaacgggg
     6721 atggcttgag tttttttgcc aaagacggaa cgctgagcgg ttttcgggtt aaccgggtgg
     6781 aagggaagag gatattcccg gcagaaatgc ctgtaacaga acctaatata ttgctttaca
     6841 gaaatcagga tcatgaattt gataaaatcc tggccggtaa aacatccgaa cgaaaaatcg
     6901 gagttgatat tcagttttca gagacatccg atggattttc aatacaaatt accgatgaag
     6961 aaggtatatc ggccggttgt gagtttgtat ccgaaaagca acctgccaat aatgccgaac
     7021 gtgctctaca gaccatcgaa agtcagctga caaaactggg aaataccatt tatacggctc
     7081 gtaacgttga tatttcggtt agcgcgcccc ggtttctgcc tgcttccgca ctcaacgagt
     7141 ggcggcggaa aaccattgaa ttgctcgacg aaaaacggct gaaagcttac atcacccgaa
     7201 aaagaaaacc ggcaaacacc gaagccgttt tccctgaaaa aaaactaact tatcttggca
     7261 atgttaccaa tgaacgggca cgtgaatttt atttgcagca tggagtggaa gagataatgc
     7321 ccggtttcga agtcatcgcc gaacccgatg tgccgttgat gttttgtaaa cattgcatta
     7381 aatattccct gggctggtgt ccgaaagaag gttacagggc cacattccgt gaaccgctct
     7441 acatcactta caaaaatcag cagtttcagc tcgaatttga ctgccgcaaa tgcgaaatga
     7501 tggtgaagaa aagaaaagat taaaaaaatc catacccgaa tcgggatatg gatttttttt
     7561 tcttttgaca gaaaataatc agataaaatt actttttgat aaacctgaat ttctcatttc
     7621 cggctgtaag tatgtaaacg ccgggtttca ggttgcttaa ttccaatcgt attttttcgt
     7681 cgttgctggt ctgttgcatc agcactttgc cggtgaggtc aattacgcgg atcagtctgt
     7741 tctgcgcatt ttcaacaatc agataattat cagccagcgt tggatacatt cgtgcggtga
     7801 ggcggatgtc gtcggtagac gtctggttgc tgttggcaag attgcaggta ggacttgtta
     7861 caagccaagt gctgctgcca tcagggatgc gtgagtagct catgttttgc tccatgtacg
     7921 ggaatgtcac tctgtccatt tcggtcagct ggcccaggta gttgaggcgc gacaaggtca
     7981 ccgtttcacc gtctttactt agtttgaaac tggcatgcaa tactccctga gacgattcat
     8041 catcggcccg gattaccagc cggcctttgg ccggaatggt ggtgagcgca gcatttgtag
     8101 ccggtatctg atacaatgta ggattggccg gtgtgtctga tatgtaccaa ccagcaatgt
     8161 tcacttcgcg gttgctgttg ttgaaaattt cgatataatc gtctttatca ccgaattcat
     8221 ctgctatgat tgtgttagaa gccacaattt cattaatcac cacctgcaga tctttgtcgg
     8281 gatcttcgcc tgagtttttc tcgtaaatgg cccggatatt aaacgtaccg gtaagtgtgg
     8341 aggcgaacac cggtgataaa attacctgtg attctcccga gatggtgcag gtaaactgaa
     8401 gattgaaaaa caaatccgaa ctattggcgc cggtttgatg tacttccacc gctatcacgt
     8461 tattgccttc ggtgaggagg tttttgggca cagtaaatgt acctgttact ccattgttgt
     8521 aagtggttga gtaggtattg aaagtaagcg ttccggcggg catgtttacc cttcccactt
     8581 cggttccgtt tacataaacg gcagcgccgt cgtccacgaa agtgcttatt tcaaaattat
     8641 ccttcgatgc cagatccgta atattcactg ttttacggaa ataggcggtg ggatatttgt
     8701 tgttcgcatc gctgccgtaa ccgattacag tggtgtgccc cagtcctccg taacccaacg
     8761 aagcgatgcc ggttttccat ccgctgtcgg agtagccggg tgaaaaccag tttgaggcgg
     8821 gcattgcatt tccatcccag tacttccaga tgctcccgtt agctatcagc atttgagaat
     8881 tggaacctgc gggtatctcc cattgtttga aggtgtatcc gggtacagat ttggcttcga
     8941 gtgtatatgc ctgattttta aagtatttca ggttgatttg agcgtcctga atggtttcag
     9001 cattgaattt
//
LOCUS       DAORKP010000093         9001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1103_length_9001_cov_8.198189, whole genome shotgun sequence.
ACCESSION   DAORKP010000093 DAORKP010000000
VERSION     DAORKP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_9001_cov_8.198189"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2260)
                     /locus_tag="PLN75_06370"
     CDS             complement(29..2260)
                     /locus_tag="PLN75_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03775.1"
                     /translation="MKKQLILLFLVSFSSVSFAQVHGIIKDNAGQGIPGAHIHWLGTD
                     VGTLSDESGHFIISQPNDANTLIFSSIGFASDTVNVAGKNNIDQVNVILDDHTELAEL
                     TVTQRRSGKYVNRLAVQNMETLNTNELRKAACCNLSESFTTNPSVDVSYSDAATGAKQ
                     IKLLGLSGSYVQMLTENIPNLRGLSSAYGMGYIPGPWMESIQISKGTASVKTGYEAIA
                     GQINVEYKKPQNSDRVSLNIFTNSVGRTEFNADAAVKLSDSLATSVMLHASDDYMTPD
                     DNGDGYRDLPRIRQYNLANRWFYQKDNYILQAFVRGLSENRTGGKTDNSYKIGINTER
                     YELFVKNGFLFGGDEHEEEGHQHDQEHEHNHEHNLSSLGIILNGTIHNQDAVYGAKKY
                     DGRQNNLYLNVIYDREMGEHHKFSAGTSFNMDTYDEILTLGNTQNFTRTEYVPGAFAE
                     YTVTYKDLTAMAGLRADYNTLYGTFVTPRFHVRYNIKDRAHLRVSAGKGYRTPNILAE
                     NNFLLASNRVINIGENLKQEEAWNFGATAHVFIPVWGGKELALMGEWFYTNFINQVVV
                     DMDSNPHAAGFYNLNGKSYSSSLQFEANLEVFRGFTATLAHRINDVKVTVMGRLREKP
                     LNNRSKSLITFTYETPLKKWQFDYTAQFNGGGRMPDPDASNPLWENEFKPFNIMNAQV
                     TRNFRTWSVYAGAENITNFMQHHPIIDVQNPFGDNFDASMVWGPMHGRTFYAGLRWAL
                     TKK"
     gene            complement(2349..2816)
                     /locus_tag="PLN75_06375"
     CDS             complement(2349..2816)
                     /locus_tag="PLN75_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03776.1"
                     /translation="MKTKAIHIILSLFFSAIFMFAGTGYNLIRYCCDDCRNTGIWKVE
                     EMSCEIVHDQHHHSCCGSGYSQTVPAPKSCVNEMNKGCDIDRLTVDVPSVQSSESEAL
                     NYSLIYSQLADYQIDVISYSSFTSDPVSHHTPPNISFPQAGRYILSKISVLII"
     gene            3013..3837
                     /locus_tag="PLN75_06380"
     CDS             3013..3837
                     /locus_tag="PLN75_06380"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HRG03777.1"
                     /translation="MRRFVVYLISTVFIAGAFISCQTQEMETEILDFSKKFYLSEDTT
                     LGGLSFQAETEIPVKFHNMEVLENVRKQIIANIFGEVFNNFPADSILPKYANNLFLDY
                     KRSNETYLANNAEIKESPLILENEIQIQGVAMYLDDRILSYSYERYAFMGGPHGNSSR
                     LLYNFDLTNSHLIKESDLFIADYTESLTRLIKQQIVEDNAEMESVADLNDFHFFEDQI
                     KPNNNFYITAEGIVYVYNPYDIAPYSTGQTVVLLTFEKLRPILKPENPIAYLYKNE"
     gene            3846..4370
                     /locus_tag="PLN75_06385"
     CDS             3846..4370
                     /locus_tag="PLN75_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRG03778.1"
                     /translation="MPFYELIKNRRSIRQYIDRKIEEEKINRLTAAALMSPSSKNSKP
                     WEFIIVENRDTLVKLSESRNQGSQMLARAPLAIVVLADTAKSDVWPEDASIAALMIQL
                     QAQDLGLGSCWVQIYQRWKDETQSSEDFIRKVLEIPEKYAVLCVISIGYSNEERLPHE
                     PEKLANDKIHREIF"
     gene            4402..4779
                     /locus_tag="PLN75_06390"
     CDS             4402..4779
                     /locus_tag="PLN75_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03779.1"
                     /translation="MSKDSRFPWGISLLVFGIFFLIRQLGIFSPEVDDLIFDLRNVLL
                     VVGVIFLITYRNKAIGIVLISIWALFYLKDLILWSRNLSDFIWPLLLIAAGALLVVSA
                     KGNKKDSTDIVKKDENHTENIEN"
     gene            4793..5284
                     /locus_tag="PLN75_06395"
     CDS             4793..5284
                     /locus_tag="PLN75_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRG03780.1"
                     /translation="MKTAIIYTTTYGTTEKVANYIAEKLHYKNIDVVELKHNTDFDVS
                     PYDIVILGASVYLGDIQHTMSNFCKRNLEVLLTKKIGLFICGIEPELVRQDAELEMAF
                     PLNLYNYALATAFVGGEINLEALNASQKFITTSLLGIKESASFIQYDLIDIFINQLQI
                     ASE"
     gene            5281..6270
                     /locus_tag="PLN75_06400"
     CDS             5281..6270
                     /locus_tag="PLN75_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRG03781.1"
                     /translation="MSSYLKLIRWENLLFIFLIQWLMQHAIIYPLLQTFGFETGQHQT
                     FYLLLIAATVLISAGGYVINDYFDVKIDRINKPAKVIVGESVSLRSAMVLYQILTILG
                     TLTGIILAFLSKSFTLGFIFILTPGLLWFYSASYKRQFMIGNLIVSLTASLSILIVGI
                     LAVALLKTQYSSELLSQTPVPSQIYGWTGGFTGFAFLLTWIREIIKDLEDAEGDREME
                     CRTMPIKWGETHTKRFLYALITFTIASLFFICFRYIHFEGTLTLRYLIFGIVIPLTVL
                     AFLIFKARRKEDFRQASTLSKLIMLAGVFYSLIFYYLQAKEFGISLFGLFLIK"
     gene            6273..6845
                     /locus_tag="PLN75_06405"
     CDS             6273..6845
                     /locus_tag="PLN75_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HRG03782.1"
                     /translation="MLTHLKKYDIILGSASPRRNELLKGLDIEFRIMSNDVDENYPAD
                     LIGVQIPEYLAQKKADTYTLKENTLLITADTIVWLDGVVYGKPTDRNDAREMLKMLSG
                     KMHQVITGVCIKTAGKSTVFHALTDVYFAPFSDEEIEYYLNHYAPYDKAGSYGVQEWI
                     GYIGVERIEGSYFNVMGLPIQRLYNELKNW"
     gene            7039..7752
                     /locus_tag="PLN75_06410"
     CDS             7039..7752
                     /locus_tag="PLN75_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRG03783.1"
                     /translation="MKIDVCFSPKLFPFYALDDQIIVITDIFRATTSICTSFHNGAKG
                     MKTVSSVEEAMQLKEKGYPVAAERNVKKCDFADFGNSPFDFTPEKVSGKDIVFTTTNG
                     TRAVEMALGADEILIGAFSNISVLTEYCSSKVKNLLILCSGWNNRFCLEDTLFAGALA
                     EKLLQNKSSDSKSDATLAALELWKIAKTDIKSYICNTEHYDRLKMNGLENSVEYCLTA
                     DTAPVLPFYDKSERKFVIE"
     gene            7781..8863
                     /locus_tag="PLN75_06415"
     CDS             7781..8863
                     /locus_tag="PLN75_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRG03784.1"
                     /translation="MKINLLLLAGMMLMGISCKKAPNSAKEEPVTPVFIKGADIGWLP
                     QMEATGYKFYNKDGKEEDCLKILKSYGINAVRLRTWVDPSDNPQSGHCSRDETVAMAV
                     RAKKEGMQVMINFHYSDSWADPAKQVKPKAWVNLSFIELKKALYDYTFDVMTALKNAG
                     IQPEWVQVGNEITPGLLLPEGNADTNFSQLVELLNQGYDAVEAVSPDSKVILHVDQGN
                     NNKRFRWWFDNAEKHKARYDIIGMSYYPYWLEGSPDYTLSIDSLGRNLNDMVSRYGKE
                     VMVVEVGGEDGQDSRLTAEQNATKIRNTHDLVAATIQKVREVPDNKGLGVFYWEPQGA
                     RSWSRYALSAWGNDGRPTEIMDAFSK"
BASE COUNT         2683 a         1863 c         1904 g         2551 t
ORIGIN      
        1 attgatttat ttgaatgctg caaattgact attttttggt cagagcccat cgcaggcccg
       61 cataaaaagt ccgtccgtgc atgggccccc aaaccatgga tgcatcaaaa ttgtccccaa
      121 aaggattttg aacgtcgata atcggatgat gctgcataaa attggtaata ttttcagcgc
      181 ccgcatacac cgaccatgtt ctgaaattcc gggtaacctg agcattcata atgttaaagg
      241 gcttaaattc attttcccaa agcggatttg aggcatccgg atcgggcatg cggccaccgc
      301 cgttgaattg agccgtgtag tcaaactgcc attttttcag cggtgtttcg tacgtgaatg
      361 taatcaggct tttcgaacgg ttattcagcg gtttttcgcg aagccggccc atcacagtca
      421 cttttacatc gttgatacga tgagccagtg tggctgtaaa tcccctgaac acttccaggt
      481 tggcttcaaa ctgcagactg ctcgagtagg atttaccgtt gaggttgtaa aaacctgctg
      541 catgcggatt ggaatccata tcaacgacta cctgattgat aaagtttgtg taaaaccatt
      601 cacccatcag tgcgagttct tttccgcccc ataccggaat gaaaacatgg gctgtagctc
      661 caaaattcca ggcttcttcc tgtttcaggt tttcgccgat gttgattacc cggttacttg
      721 ccagcaaaaa gttgttctcg gcaagtatat tgggtgtccg gtaccctttt ccggcagaaa
      781 ctctcaggtg agcgcggtct ttgatgttgt agcgcacatg aaaacgcggg gtaacgaacg
      841 taccgtagag cgtattgtaa tcagccctta atccggccat ggccgtgaga tctttgtaag
      901 taactgtgta ttcggcaaaa gctcccggaa catattccgt ccgtgtgaaa ttctgcgtat
      961 tccccagcgt cagtatttca tcgtaggtat ccatgttgaa actcgttccg gcagaaaatt
     1021 tatgatgctc acccatttca cggtcgtaaa ttacattcag ataaaggtta ttttggcggc
     1081 catcgtattt tttggctcca tatacagcat cctgattgtg aatggttccg ttaagaatta
     1141 ttccaagact ggataggttg tgctcatgat tgtgctcgtg ttcttggtcg tgctgatgtc
     1201 cttcttcttc atgttcatca ccgccaaaca gaaaaccatt tttcacaaac agttcgtatc
     1261 gctcggtgtt gattcctatt ttgtatgagt tatcggtttt ccctccggtt ctgttttcag
     1321 aaagtccccg cacaaatgcc tgcagaatgt aattgtcttt ctggtaaaac caccggtttg
     1381 ccaggttgta ctgacgaatc cggggtaaat cccggtatcc gtctccgttg tcgtcgggtg
     1441 tcatgtagtc atcagaagca tgcagcatca ccgatgttgc cagcgagtca ctgagtttga
     1501 cagccgcatc ggcgttaaac tccgttctgc caacactgtt cgtaaatatg ttcagagaga
     1561 ccctgtcgct gttctgaggt tttttatatt ctacgttgat ttggccggca atggcttcat
     1621 atccggtttt gaccgatgcg gttcccttgg aaatctggat actttccatc cacggaccgg
     1681 gaatgtaacc cataccgtaa gccgacgaaa gaccgcgaag gttggggatg ttctcggtga
     1741 gcatttgaac gtagctgccc gacagcccga gcaatttaat ctgtttggca cccgttgccg
     1801 catcgctgta ggatacatct accgaaggat tagtggtgaa actctccgaa aggttgcaac
     1861 atgcagcttt tcggagttcg ttggtattga gagtttccat gttttgtacg gccagtcggt
     1921 taacatattt cccggatctg cgctgcgtta cagtcagttc agcaagttct gtgtggtcgt
     1981 cgaggataac atttacctga tcaatgttgt ttttaccagc cacatttacc gtatcggaag
     2041 caaagccaat gctgctgaat atcagcgtat tggcatcatt gggttgtgaa attataaaat
     2101 gtccgctttc gtcggatagt gtgcccacgt cagttccgag ccagtgtatg tgtgctccgg
     2161 gaataccctg acctgcattg tcctttataa taccatgtac ctgggcaaag gaaacactgg
     2221 aaaaggaaac taaaaaaagt aatattagtt gttttttcat ttgtatattt gaatattttt
     2281 aaaaaataaa gaatagtgat tcagacatct atgtatctaa aaatcaaacg atttatttta
     2341 taaaataatc aaataatgag cacggaaatt ttggaaagaa tgtatcttcc cgcctgtgga
     2401 aaagaaatat tgggtggtgt gtgatggcta accggatctg acgtgaaaga ggaatacgaa
     2461 atcacatcta tttgataatc agctaattgc gaataaataa gagaataatt gagagcttct
     2521 gattctgaag attgcactga aggaacatcg acagtgagac gatcaatatc gcatcccttg
     2581 ttcatttcat tcacacatga ttttggagcg ggtacggttt ggctatagcc cgaaccacag
     2641 caggagtgat gatgttggtc gtgtacgatt tcacacgaca tttcttcaac cttccatatt
     2701 ccggtattcc ggcaatcatc acagcagtaa cgaatgaggt tatagcctgt tccggcaaac
     2761 atgaaaattg cagaaaaaaa caaacttaaa atgatatgta ttgcctttgt tttcataagc
     2821 gactgcaaat ataattcaaa cgtgattcaa ttcaaaccta ttctccaatt atttaggatt
     2881 attttaaata atattttaaa aaatgatgaa taaattattg ggactaaaat cacaatttgt
     2941 cagataatat tgaaccaatt gaaaggaatt attagtaaat ttgcagaaat ttttaaaaat
     3001 gtataattga ttatgagaag gtttgttgtt tacttaattt caaccgtttt cattgccgga
     3061 gcattcatat cctgtcagac tcaggaaatg gaaactgaaa ttttagactt tagcaaaaaa
     3121 ttttacctga gtgaagacac tactctgggt ggtttgtcat tccaggctga aactgaaatt
     3181 cccgtgaaat ttcataatat ggaagtgttg gagaatgtaa gaaaacaaat cattgccaat
     3241 attttcggcg aagtgttcaa taactttccc gctgactcca ttctccccaa atatgccaac
     3301 aatctttttt tggattataa aaggagcaat gaaacctatc tggccaataa tgcagaaata
     3361 aaggaatcac ctttaatact tgaaaacgaa attcagattc agggagtagc catgtatctg
     3421 gacgacagaa ttttatctta cagttacgag cgctatgcct ttatgggagg ccctcacggt
     3481 aacagcagcc ggcttcttta caactttgac ctcacaaatt cgcatttaat caaagaaagc
     3541 gatctgttca tcgccgatta caccgaatca ctcacccggc ttatcaagca gcagatagtg
     3601 gaagataacg ccgaaatgga atcggtagcc gatttgaacg attttcattt tttcgaggat
     3661 cagattaagc caaacaataa tttttatatc acggctgaag gaattgtata tgtgtataat
     3721 ccttatgaca ttgcacctta cagcaccggt caaacggtgg tattgcttac gtttgaaaag
     3781 ctcaggccta ttctgaaacc tgaaaatccg attgcatacc tttacaaaaa cgaataagtt
     3841 tgattatgcc tttttatgaa ctgataaaaa accgcagaag cattcgtcag tatattgaca
     3901 ggaagattga agaggagaag attaaccggc tcacagcagc tgcacttatg tcgccttcgt
     3961 caaagaacag caagccgtgg gagtttatta tcgttgagaa tcgggacacg ctcgttaagc
     4021 tatccgaaag ccgcaaccag ggttcacaga tgcttgcccg cgcaccgctg gccattgtag
     4081 ttttggcaga taccgccaaa agcgatgtgt ggccggaaga tgcttccata gcagcgctta
     4141 tgattcagct tcaggctcag gatcttggat tgggaagctg ctgggtgcaa atataccaac
     4201 gctggaaaga tgaaacgcag agcagtgaag attttattcg taaagttctt gaaattccgg
     4261 agaaatatgc cgtattatgt gttatatcta tcggatacag caacgaagag cggctacctc
     4321 atgagcccga aaaacttgca aacgataaaa ttcatcgcga aatattttaa ctaatactca
     4381 ctttttaatt cattaaatgc catgtcaaaa gacagtcgtt tcccctgggg aatttcatta
     4441 ctcgtatttg gaatattttt tctgatcagg caactcggta tattctctcc cgaagtagat
     4501 gatttaattt ttgatttgcg aaatgtgttg cttgtagttg gagtcatttt tttgattacc
     4561 tacagaaata aagctatcgg gatagtgctg atatccatct gggctttatt ttatctcaag
     4621 gatttaatct tgtggtccag aaatctttcc gatttcatct ggccattatt gctcattgca
     4681 gccggcgcat tactcgtagt gagcgccaaa ggcaataaaa aagactccac tgacattgta
     4741 aaaaaagacg aaaatcacac agaaaatatt gaaaactaaa aaatacagcc cgttgaaaac
     4801 agccataatc tacacaacca cttacggtac taccgaaaaa gtagcaaatt atattgccga
     4861 aaagctgcat tacaagaaca ttgatgtcgt tgaactgaaa cataacacag attttgatgt
     4921 atcgccttac gatattgtta tattaggtgc ttcagtttat ctgggtgata tacagcatac
     4981 tatgagcaat ttttgcaaaa gaaacctgga ggtactgcta accaaaaaaa tcggattgtt
     5041 catttgcggc atcgagcccg agctcgtgag gcaggatgcc gaactggaaa tggcgtttcc
     5101 gctaaatctt tacaattatg cgctggctac agcattcgtg ggtggcgaga ttaacctgga
     5161 agcattgaac gcatctcaaa aattcatcac cacatcattg ctcggaatca aggaatctgc
     5221 ctcatttatc caatacgatc tgattgatat tttcattaat caactgcaaa tagcaagtga
     5281 atgagttcat acctgaaact gatacgatgg gaaaatctgc tgtttatctt cctgattcag
     5341 tggttgatgc aacatgctat aatatacccg ttgcttcaga ccttcggatt tgaaacagga
     5401 cagcatcaga ctttctactt attgcttata gctgctaccg ttttgatttc ggcaggcggc
     5461 tacgttatca acgattattt cgacgtaaaa atagaccgaa tcaacaaacc tgcaaaagtg
     5521 attgtaggtg aatccgtttc actccggtca gcgatggtgc tgtatcaaat tcttaccata
     5581 cttggcactc taacaggcat aatactggct tttctgtcca aaagttttac actcggattt
     5641 atttttatcc tcacaccggg gttgctctgg ttttattccg caagttacaa acggcagttt
     5701 atgattggaa atctgattgt cagccttacg gcctcacttt ccattctgat agtgggaatt
     5761 cttgcggttg cccttctcaa aacacaatac agcagcgaac ttctcagtca aacaccagta
     5821 ccttcacaaa tttacggctg gacaggtggt ttcacaggtt ttgcatttct gcttacctgg
     5881 atcagagaaa taattaaaga cctggaagat gcagagggcg accgcgaaat ggagtgccga
     5941 accatgccaa taaaatgggg cgaaacccat acaaaacggt ttctgtatgc attaatcacg
     6001 tttacaatcg cttcactgtt tttcatttgc tttcgttaca tccattttga aggcaccctc
     6061 accctgcgtt accttatttt cggaatcgtc ataccgctca cagtccttgc ttttcttatt
     6121 ttcaaagcca ggagaaaaga agatttcagg caagcctcta cactatccaa actcatcatg
     6181 ctggcaggtg ttttttacag tctgattttc tactatcttc aggctaaaga attcggtatc
     6241 agcctgttcg gactttttct tataaaataa ttatgcttac acacctgaaa aaatacgaca
     6301 taattcttgg ctctgcatcg cccagacgta atgaactgct aaaaggtctg gacatcgaat
     6361 tccggattat gtccaatgac gtggacgaaa attacccggc cgacctgatt ggagtacaaa
     6421 ttcccgaata tctggctcag aaaaaagccg acacctatac actaaaagaa aacacactgt
     6481 tgattacagc cgacaccatc gtttggctcg atggtgtggt gtatggtaaa cctaccgacc
     6541 ggaatgatgc acgtgaaatg ctgaaaatgc tttcgggaaa aatgcaccag gtcattaccg
     6601 gcgtttgcat caaaacggca ggaaaaagca cggtttttca cgcattgaca gatgtatatt
     6661 ttgctccgtt ttctgacgaa gaaattgagt attatctgaa tcattacgcc ccctacgaca
     6721 aagccggctc ctatggcgtg caggagtgga taggttatat cggcgttgaa cgtattgaag
     6781 gctcgtactt taacgtcatg ggacttccca ttcagcggtt gtataacgaa ctgaaaaact
     6841 ggtaaataaa attttgagtt tcgggtttcg gatttcggat tctgagtttt gtattcaatg
     6901 ataaataaac attcaaaagt cttcgacgtt ttgaagtttg tcaacatcgt atcttctgtg
     6961 ttccaagctc catattccaa gtactatgtt ctgtatttgt tcctggttgt tattgttgat
     7021 ttttaaaaat gctttttcat gaaaattgat gtttgctttt cacccaaact ttttccgttt
     7081 tatgccctcg atgaccagat aatagtcata acagatattt tcagggctac caccagtatt
     7141 tgcacttcgt tccacaacgg agcaaaaggc atgaagacag tttcatccgt tgaagaagcc
     7201 atgcagttga aagaaaaagg ttatccggta gctgccgaaa gaaatgtaaa gaaatgtgat
     7261 tttgctgact tcggaaattc tccctttgat ttcacgcccg aaaaagtcag cggaaaagat
     7321 atcgttttta caactaccaa tggtacaaga gctgtagaaa tggctctcgg tgcagatgaa
     7381 atactgatag gtgctttttc aaacatttcg gtactgacag aatattgttc atcaaaagtt
     7441 aagaacttac tgatactttg ctccggatgg aataaccggt tttgcctgga agacaccctt
     7501 tttgccggag cacttgcaga aaaattactc cagaataaat cttccgatag taaatctgat
     7561 gcaacacttg ccgctctcga actttggaaa attgcaaaaa cggatataaa atcttatatt
     7621 tgcaataccg aacactacga caggctgaaa atgaacggac tggaaaactc ggttgaatat
     7681 tgcctgacag ctgatacagc acctgtttta cccttttacg acaaatccga aagaaaattt
     7741 gtaatagaat aaaatcattt ttctaaaaaa aaacaccaga atgaaaatca acttattact
     7801 gttagcagga atgatgctga tgggaatttc ctgcaaaaaa gcacccaatt ctgcaaaaga
     7861 agaaccagtc actccggtat ttataaaagg agcagatatc ggctggctgc cgcaaatgga
     7921 agccacagga tataaatttt acaacaagga cggaaaagaa gaagattgcc tgaaaatcct
     7981 gaaaagctac ggaatcaatg ccgtacgcct ccgcacgtgg gttgaccctt cggacaatcc
     8041 tcaaagcgga cattgcagcc gcgacgaaac cgtggcgatg gccgtgaggg caaaaaaaga
     8101 aggcatgcag gtgatgatta atttccatta cagcgacagt tgggcagatc cggcaaaaca
     8161 ggtgaaaccc aaagcctggg taaatctcag ttttattgaa ctaaaaaaag cactctacga
     8221 ttacactttc gatgtgatga ctgccctaaa aaatgcagga atacagccgg aatgggtaca
     8281 ggttggaaat gagattactc cgggacttct gttgcccgaa ggcaatgctg acaccaactt
     8341 ttcgcagttg gttgagctgc tcaatcaggg ttacgatgca gtagaagcag taagtcctga
     8401 ttcaaaagta attcttcacg tagatcaggg aaacaataac aaacgattcc ggtggtggtt
     8461 tgacaacgct gaaaaacaca aagcccggta cgacatcatc ggcatgtcgt actaccccta
     8521 ctggctcgaa ggaagcccgg attacacact atccatcgat tcgctcggca gaaatctgaa
     8581 tgacatggtg agccgctacg gaaaagaagt gatggtggta gaagtaggcg gcgaagacgg
     8641 gcaggacagc cggctcacgg cagagcaaaa cgcaacaaaa atccggaaca cacatgatct
     8701 ggtggctgcc accatccaga aagtgcgtga agtacccgat aacaaaggac ttggagtatt
     8761 ctactgggag ccgcagggag cacgcagctg gagccgttac gcactgagcg cgtggggtaa
     8821 cgacggcaga ccaaccgaaa ttatggatgc attttcaaaa taaaattttc gaaaaatatt
     8881 gaacaaatga gtctttacgg ttgttttaag ggcaataatt ttcattttaa gaaataaatt
     8941 aaaaaacaat aataaataaa aggagaacaa aactatgctt aatccaaaaa ttgcaaaaat
     9001 g
//
LOCUS       DAORKP010000094         8990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1105_length_8990_cov_8.351203, whole genome shotgun sequence.
ACCESSION   DAORKP010000094 DAORKP010000000
VERSION     DAORKP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1105_length_8990_cov_8.351203"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..681
                     /locus_tag="PLN75_06420"
     CDS             37..681
                     /locus_tag="PLN75_06420"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HRG03785.1"
                     /translation="MKLNINKLVFFSLLIFNINFGVQAQDIIKYFTDMPAYLLPSLES
                     SHKLELIENYQKTGKDTLENLFGGKVTLLDLDTVADYISIRTTSVSRFEMMLFQREDK
                     SSFITVINTVCAPVCSSYIHFFDTEWKEIRMEFPKFDNRQWLKSTDETIDGVKLQDMI
                     KGIFLEFNFNPHEKSVEVRNNSVQMLGKTEQGQIKPFFMEDKIISVKWKEGKWR"
     gene            721..1740
                     /locus_tag="PLN75_06425"
     CDS             721..1740
                     /locus_tag="PLN75_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03786.1"
                     /translation="MIDNFPLVSIISPTYNHEKYIADCIESVLNQTYSNWEMIIIDDG
                     STDATFTIAEKYAGKDNRIQAYTQKNIGIFRLGESYNFALSKCRGKYIAILECDDVWL
                     PEKLSIQVDVLEKRPECVLSWGKAYLSSVDLSENYYLAPQNMDDEKLFNNKPVGVFLK
                     RFIYSTLIPALTIVIRKEKLDEIGGFIQKHGLPLVDIPTTLELLLRGEFAFVGQPLGH
                     WRIYPNQVTKTYTGQMTASYYLLIQELMARHPGLFESQGLTKKEIDNHFADRLVVSYS
                     RSGRYKLIRKDFKGARKDYIYSITHYGFHEPVWKLRSLVGLVMSWFHGDVEWIAKLIG
                     NASYK"
     gene            1758..2660
                     /locus_tag="PLN75_06430"
     CDS             1758..2660
                     /locus_tag="PLN75_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03787.1"
                     /translation="MKSLKLIWQKSAPLFIQNFLNIFTDSLENKAKRKKILNYYRNKA
                     KNKLTWEESELIRFLKHHRYSPFPFQWALKYDNLIPEVLFDESVSFHYVLFEGKKLYF
                     PRNFTRNQVMWTMRSISKEQDKKSAHLYLTDAFQVENGSIMVDGGVAEGSLSLSLIEK
                     LKKLYLIECEPNWNEALKLTFAPWKEKVEIVTKYLSDITDEQHVSIDNLVEVKEEEKY
                     FIKLDVEGYEKQTLKGMNRFFSQVKNLKMCVCTYHFENDAVEISKMLENREMSYEFSE
                     GWLLFSMENEMPSFRKALIRAWKE"
     gene            complement(2665..3807)
                     /locus_tag="PLN75_06435"
     CDS             complement(2665..3807)
                     /locus_tag="PLN75_06435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03788.1"
                     /translation="MKVLFLSSWYPSAENPAFGVFVKEHAKAIHTTSAEIVVLAVLVF
                     RSGDLLKIHVKDYKDESGIRTIEIIIRSRYRDIIHHLVWFQKQIVYHYFRKLILKNFQ
                     PDIIHSNVIFPAGITGDFIARKINKPHIITEHWSKIGDLLRKPVFSASARKAYTKARA
                     ILPVSEFLRNHLLDLLPDLPPTKLRVVPNIIDSETFAYVEKEVSDELRFCALATWATK
                     RKPDKLPELFIEALNNFHLKTGKKVRLTMLGGGDRLDELKALASKQSYPIEFAGSIPK
                     PDIALQMQKADFFVHASTIETFGVVVAEALMTGTPVICSRVAALPELVNQSNGVLCEN
                     SVESWVDGIEKAVITTFDNQKIAQTVRDKFSYRAVGRKIEDIYKDL"
     gene            complement(3895..5352)
                     /locus_tag="PLN75_06440"
     CDS             complement(3895..5352)
                     /locus_tag="PLN75_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRG03789.1"
                     /translation="MGDNLKEKMIGAVAWTSIDRIGMQAVQFVVGLILARLISPENYG
                     LIGMVMIFLPVSTLLVDGGFGLALVRKQNADEMDFNTVFLFNIFVSVILYLFLFLAAP
                     LIAAFFNEPQLVNISRVLFLIILINSLYLIPIAKMQRNLDFRGSAKVNIFSILCSGII
                     SVILAFSGFQVWALVLQQVLFHFFRLIIFQYYVKWKPKFMFSFRVIRDMGAFSLHLLG
                     TNVLNAVFNQIYIVILGKFYSKYETGLYTQANKLNETTNSTFQTILVGSTYSLLVKIQ
                     DDEERFRRVYRGIASKISIVTFPFMFVLVSVAFPLIFVLYSEIWVKAVPYFQLLTLSA
                     LFAPLYGLNVNALNARGKSNITFRVEIIKKTLILISVLICFRWGIMAMLTGYIAACYI
                     AYFISMIYLKLNLKHKLSHQIADFVGCIGVGLVLALSNTGISFLIENNYLLIALQLTA
                     SAIIYLIFVRLFYNELFSQALHFVFEKTKLKKNNR"
     gene            complement(5425..7542)
                     /locus_tag="PLN75_06445"
     CDS             complement(5425..7542)
                     /locus_tag="PLN75_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRG03790.1"
                     /translation="MKKITLFLLGILLTVGVKADEGMWMLPLVEKLNIQKMQGLGLTM
                     TAEQIYSNNNTSLKDAVVIFGGGCTGVMVSPAGLLFTNHHCGYSAIQQLSSVEHNYLK
                     DGFTAAELTDELPCKGLAVTFLVKVEDVTPRVLEKTGNLTGKEREKALNEIIETIRKE
                     AKGETNYTAQVRSFYSGNEYYLFVYERFNDVRLAYTPPSSIGKFGGETDNWMYPRHTG
                     DFSVFRVYADKDGKPAEYSDKNIPYVPKRFAAVSNKGMSEGDYAMILGNPGSTDRYAT
                     SFAIKNRVKNGNSARIEMRGVKQEVWKKFMRADEAINIAYANKFAGSSNYWKNSIGMN
                     NAIKNLNVIAEKEKQEAEFTEWINSNVSLKQKYGNILPELKRDYELIDKNQKAIEYLR
                     ESLLAGVELPRMASGVDRMDKKLPLDSNMAFISNIYKDYYPEVDKNAFTALLEAYRKS
                     VTPDALPEIYKTIDKKFKGNYSKYTDYLFSKSVFTSQDKLVKALKKSKYDLTKDPVAV
                     FGKDVQKKMTELRTKEYDEALANIDNNKRLYEAALKEWYAAKDKSMYPDANFTMRLTY
                     GQILGYKPADGVDYEFYTTTKGVLEKEDPENPEFVVPEKLKEAIEKGDYGRYIDTKTG
                     KMHVAFISTNDITGGNSGSPVFNAAGEVIGLAFDGNWEAMSGDIIFEPNLQRTISVDI
                     RYVLFVMEKVGGADRLIKELTIK"
     gene            complement(7865..8884)
                     /locus_tag="PLN75_06450"
     CDS             complement(7865..8884)
                     /locus_tag="PLN75_06450"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRG03791.1"
                     /translation="MENIDWSNLGFGYMKTDYNVRCSYKDGEWGEIRTCTEETITMHM
                     AATSLHYGQEAFEGLKAFRGKDGKIRIFRMNENAKRLQSSCDGIMMAKLPVEKFEEAV
                     LKVVKLNERFIPPYESGAALYIRPLLIGTSAQVGVRPAKEYEFLIFVTPVGPYFKGGF
                     ATTPYVIMREYDRAAPLGTGTFKIGGNYAASMVAGMKAHDMGYSAVFYLDAKEKKYID
                     ECGAANFFGIKNDTYITPKSSSILPSITNKSLMVLAAEMGMKVEQREVPEEELATFEE
                     AGACGTAAVISPIERIDDLLENKSYIFSNDGKPGPWCTKLYNKLRAIQYGDEPDTHGW
                     VTVLE"
BASE COUNT         2605 a         1811 c         1828 g         2746 t
ORIGIN      
        1 ctatacatcc ttaatttttt ttaaaaaaat cttcgtatga aattgaatat caataaatta
       61 gtgttttttt ctttattaat tttcaatatt aactttggag tacaggctca ggacataatc
      121 aaatatttta ccgatatgcc tgcttatctg ctcccttcgc tggagagttc acacaagttg
      181 gaattgattg aaaattacca gaaaaccgga aaagataccc tcgaaaatct gtttggagga
      241 aaagttacat tacttgatct cgatacagtg gctgattata tcagtataag gacaacatct
      301 gtttcgcgat ttgagatgat gctgttccaa cgcgaagata aatcttcttt catcaccgta
      361 atcaacaccg tttgcgcgcc tgtatgcagt tcctacattc atttttttga tactgaatgg
      421 aaagaaatca ggatggaatt tccgaagttt gacaaccggc agtggctgaa atcaaccgat
      481 gaaacgattg acggagtaaa attgcaggat atgattaaag gtatatttct ggaattcaat
      541 tttaatccac acgaaaaatc ggtggaagtg aggaataata gtgtacaaat gcttggaaaa
      601 acagaacagg gacagataaa accatttttc atggaagata aaattatcag tgtaaagtgg
      661 aaagaaggaa aatggagata atgaaacaca agaataaaaa acgtaaaaaa actgaactat
      721 atgattgaca actttccttt ggtttccatc atttcaccta cttataatca tgaaaaatac
      781 attgctgatt gcattgagtc ggtgctgaac caaacatact ctaactggga aatgataatc
      841 attgacgacg gtagcacaga tgcaacattt accatagcag agaaatatgc aggtaaggat
      901 aaccgcattc aggcatatac tcaaaaaaat atcggaatct tcagactcgg tgaatcttat
      961 aattttgcct tatcaaaatg taggggaaaa tatattgcca ttcttgaatg tgacgatgtg
     1021 tggctgcccg aaaaactatc aattcaggtg gatgtattgg aaaaaagacc tgaatgtgtt
     1081 ctttcgtggg gtaaggcata tttgtcgtct gtcgatcttt cggaaaatta ttacctggct
     1141 ccgcaaaaca tggatgatga aaaacttttc aacaataaac cggtcggcgt tttcttaaaa
     1201 agatttattt attccacgct tattcctgca cttactatag ttatacgaaa agaaaaactg
     1261 gatgaaatag gaggatttat tcagaaacac ggacttccgc tggtggatat acccactact
     1321 ctcgaactgc ttctgagagg cgagtttgct ttcgtcggac agccgctcgg acactggcgt
     1381 atttacccca atcaggtgac caaaacgtat accggacaaa tgacagcaag ctattatctg
     1441 ctcattcagg aattgatggc caggcatccc ggattatttg aaagccaggg tcttactaaa
     1501 aaggaaatcg ataatcattt tgccgacagg cttgttgtaa gttattccag atcaggccga
     1561 tacaaactta ttcgaaaaga tttcaaaggt gcacgtaaag attatattta ctccattaca
     1621 cattacggtt ttcacgaacc ggtttggaaa ctgagatcgc tcgttggttt ggtcatgagt
     1681 tggttccacg gcgatgttga gtggattgca aaattaatcg gaaatgccag ctacaaataa
     1741 ctaattgtaa ttgaaaaatg aaatctctaa agcttatctg gcaaaagtca gcaccacttt
     1801 ttattcaaaa tttcttaaat atattcacag attctcttga aaataaagcg aaaagaaaga
     1861 aaattctgaa ttattacaga aacaaagcca aaaacaaact tacctgggaa gaaagtgaac
     1921 tcatccggtt tctgaaacat catcgttata gcccgttccc ttttcaatgg gcgctgaaat
     1981 acgataatct gatacctgag gtgctattcg atgagtcggt ttctttccac tatgtgctgt
     2041 ttgaaggtaa aaaactttac tttccccgaa actttactcg aaatcaggta atgtggacca
     2101 tgcgaagtat cagcaaagaa caggataaaa aatcggcgca tctttatctt accgatgctt
     2161 ttcaggtcga aaacggaagt atcatggttg atggtggagt tgccgaagga agtctgtcgc
     2221 tttcgctcat cgaaaaactg aagaaactgt atctgataga atgtgaacca aactggaatg
     2281 aagctttgaa actgaccttc gccccctgga aagaaaaagt ggaaatcgtc accaaatatc
     2341 tttctgatat aactgatgaa caacacgtat caattgacaa cctggtggaa gtaaaggagg
     2401 aagagaaata ttttattaaa ctcgatgtgg aaggctatga gaaacagacg ctgaaaggga
     2461 tgaacagatt cttctcacag gtcaaaaacc tgaaaatgtg tgtctgtacg tatcattttg
     2521 agaacgatgc agtagaaatc agtaagatgt tggaaaacag agagatgtct tacgagtttt
     2581 cagaaggttg gctgcttttt tcgatggaaa acgaaatgcc gtcttttcgg aaagctttaa
     2641 tcagagcctg gaaagaatag actgttataa atctttgtaa atatcctcaa ttttccgccc
     2701 aacagcccgg tagctgaatt tatccctaac tgtttgagct attttttggt tatcaaacgt
     2761 agttattaca gctttttcaa taccatctac ccaactttcg acagaatttt cgcacaatac
     2821 tccgtttgat tgattaacca gttccggaag tgcagctacc cgggagcaaa ttaccggagt
     2881 tccggtcatc agtgcttctg caaccaccac tccaaaggtt tcaatggtac tcgcatgaac
     2941 aaaaaagtcg gctttttgca tttgtagtgc tatgtcaggt tttggaatag aacctgcgaa
     3001 ttcaatcgga tagctttgct ttgaagccag cgctttcagc tcatcaaggc ggtcgccacc
     3061 acctaacatt gtcagtctga cttttttccc ggtttttaga tgaaaattat ttaatgcttc
     3121 gataaatagt tccggaagtt tgtcgggctt acgtttggta gcccatgttg ccagtgcaca
     3181 aaaccggagt tcatccgaaa cttctttttc aacgtatgcg aatgtttcgc tgtcgataat
     3241 attcggaaca acccggagtt tcgtcggtgg taaatccgga agtaaatcga gcaggtgatt
     3301 tctcagaaat tcagatacag gaagtattgc gcgggctttc gtgtaggctt tgcgggctga
     3361 tgctgaaaaa acgggttttc gtaataaatc gccaattttg ctccagtgtt cggtgatgat
     3421 atgaggcttg ttaatctttc gggcaataaa atcgccggta attccggccg gaaatatgac
     3481 attggaatga ataatatcgg gctggaagtt ttttaaaatc aattttctga aataatgata
     3541 tacaatttgc ttctgaaacc aaaccaaatg atggataata tcccgatatc ttgagcggat
     3601 aatgatttca attgtccgaa taccactttc atctttgtaa tctttcacat gaattttcaa
     3661 taaatcacca gaacgaaaaa ccagcactgc cagtaccaca atttcagcag aagtagtatg
     3721 tatggcttta gcatgctctt ttacaaatac accaaaagcc ggattttcag ccgacggata
     3781 ccacgaactt agaaaaagta ctttcatgcg tttattttct atttttatat taaggacaca
     3841 tacaacacac cattaatcca aattgtgtgt caagaatagt tttagatgac tgtttcatct
     3901 gttgtttttt ttgagttttg ttttttcaaa aacgaaatgc agggcttggg aaaataactc
     3961 attgtaaaat agccgtacga agataagata gattattgct gaagctgtaa gttgcagtgc
     4021 gatcaggagg taattatttt caatcaaaaa tgaaatacct gtgttactca atgccaaaac
     4081 aagtccgacg ccgatacaac ccacgaaatc agctatttga tggctcagtt tatgtttcag
     4141 gtttaatttt aagtaaatca ttgaaatgaa atatgctatg taacatgccg caatgtagcc
     4201 agtcagcata gccataattc cccaccggaa acatatcagt accgaaatca gaatgagcgt
     4261 ttttttgatt atttccactc tgaatgtaat atttgattta cctctcgcat tgagggcatt
     4321 tacattcagt ccgtacaagg gtgcaaaaag ggctgaaaga gtaagcaact ggaaataagg
     4381 caccgctttc acccaaatct cagagtacag gacgaaaatc agtggaaaag cgacactcac
     4441 cagcacaaac atgaaaggga aagttacaat cgaaattttt gaggcaatgc ctctgtaaac
     4501 cctgcgaaaa cgttcttcat catcctgaat ttttaccaga agcgaatatg tactgccaac
     4561 cagtattgtc tgaaaagtgg aattggtcgt ttcgttgagt ttgttggcct gtgtgtaaag
     4621 ccctgtttcg tattttgaat aaaacttacc cagaataacg atgtaaatct gattaaatac
     4681 agcgtttaat acgtttgtgc caagtagatg caggctgaaa gctcccatat cccgaatgac
     4741 gcgaaaagaa aacatgaatt tcggcttcca ctttacatag tactgaaaaa tgatcagccg
     4801 gaaaaaatga aataaaacct gctgtaaaac cagtgcccat acctgaaatc cgctgaatgc
     4861 aagtattacg cttatgattc cgctgcaaag aatggaaaaa atattgactt ttgcacttcc
     4921 cctaaagtca agatttcgct gcattttagc tatcggaatg agatacaatg aattgattaa
     4981 aataatcaga aacaaaaccc tggatatgtt gacaagctgg ggctcattga aaaatgcagc
     5041 aatcaaaggt gcggctaaga acaagaaaag gtaaagaatg acggatacaa agatattgaa
     5101 caggaaaacg gtattgaaat ccatctcatc agcattctgt ttgcgtacca atgccagacc
     5161 gaaacctcca tcgaccagca atgtggaaac aggcaaaaaa atcatcacca tgcctatcaa
     5221 accgtaattt tcgggtgaaa tcaatcgggc taaaataagc cccacaacaa actgaactgc
     5281 ctgcatcccg atccggtcaa tcgaggtcca ggcaacagca ccaatcattt tttccttcag
     5341 attatctccc atattagttg caaaaaaaac agattacaaa gatagccaat gtaatctgtt
     5401 ttttttaaag aatgctctga taaattattt tatagtcagt tctttgatca accggtcggc
     5461 tccgcccact ttttccatca cgaaaagtac gtaacggata tccacgctga tagttcgttg
     5521 caaattgggc tcgaaaatga tgtcgccgct catggcttcc cagttaccat cgaatgccag
     5581 cccgatgact tcacctgctg cattaaatac gggactgccc gaatttccac ccgtgatatc
     5641 gtttgtgctg ataaatgcca catgcatttt tccggttttg gtgtcgatgt atcgcccata
     5701 atcgcctttt tcaatggctt ctttcagttt ctcgggcaca acaaattccg gattttcagg
     5761 atcttccttt tcaagaacgc ctttggtagt ggtgtaaaat tcgtaatcaa ccccatctgc
     5821 cggtttgtat cccagtatct gaccgtatgt aagccgcatg gtgaaattgg cgtccgggta
     5881 catcgatttg tctttggcag cataccattc tttcagagcg gcttcgtaga gtcgtttgtt
     5941 gttatcaatg tttgccaatg cctcgtcata ttcctttgtg cgcagttcgg tcattttctt
     6001 ttgaacatct ttaccgaaaa ctgctaccgg atcttttgtt aaatcatact tgctcttttt
     6061 caacgctttc acaagtttat cctgcgaagt gaacaccgat ttgctgaata aatagtcggt
     6121 gtattttgaa tagttgcctt tgaatttttt atcaatggtt ttgtagattt cgggcagagc
     6181 atccggtgtt accgatttgc ggtaagcctc cagcaatgca gtgaaagcat tcttgtccac
     6241 ttcggggtaa taatccttgt agatattgct tataaaagcc atattggagt ccagaggcag
     6301 ttttttgtcc attctgtcca ctccacttgc cattcgcggc aattccacac cggcaagcag
     6361 tgattcacga agatattcaa tagccttctg gtttttgtca atcaactcgt agtctctttt
     6421 cagttcgggg agtatgttgc cgtatttttg tttcagcgaa acgttactgt ttatccattc
     6481 tgtgaattca gcttcctgtt tttccttttc ggcaatgacg ttcaagtttt ttatggcgtt
     6541 attcatacct atgctgtttt tccagtagtt ggagcttccg gcaaatttat tggcgtaggc
     6601 tatgttgatg gcttcgtcgg cccgcatgaa ttttttccat acctcctgtt ttacgccgcg
     6661 catttcaatg cgggcgctgt tgccgttttt cacccggttt tttatggcaa aagaggtagc
     6721 atagcggtcg gtcgaacccg gattgcccag tatcatggcg taatcgcctt cgctcatacc
     6781 tttgttggat actgcagcaa aacgtttagg tacataagga atgtttttat cggaatattc
     6841 tgccggtttt ccatccttat cggcatatac gcggaatacc gagaaatcgc ccgtatggcg
     6901 cggatacatc cagttgtcgg tttcaccgcc gaattttcca atcgatgatg gaggtgtgta
     6961 ggcgagtctt acatcgttaa atctttcata tacaaaaaga taatactcgt ttccggaata
     7021 gaaagatcgc acctgtgccg tgtagtttgt ctcgcctttg gcttctttcc ggatggtttc
     7081 gatgatttca ttcagcgctt tttcgcgttc ttttccggtc agattgccag ttttttcaag
     7141 tacacgcggt gtcacatctt ccacttttac cagaaaagtg accgccaatc ctttacacgg
     7201 cagttcatcg gtaagttcgg cagctgtaaa tccgtctttc aggtaattgt gttccacgct
     7261 gctcagttgc tgaatggcgc tatagccgca atggtggttg gtaaagagta gtccagcagg
     7321 cgataccatt actccggtgc atcctccgcc aaaaattact acggcatctt tcaggctcgt
     7381 gttgttgtta ctgtagattt gttctgcggt cattgtcaga cctaaaccct gcattttttg
     7441 gatgttgagt ttttccacca gaggcagcat ccacattcct tcatcggctt tcacgccaac
     7501 ggtcagtaaa attccaagca aaaataaagt tatttttttc ataaatgagt aagttcatct
     7561 ccaccgggag tggatctgga taattaaatg tttttttttg tatgaaagta tgttgttttt
     7621 taaaataaac taatgacagt tgagtctgat ccgaaaacct tgtttttgcc acaaagacac
     7681 taaaacacca agttacacaa agaaactaat tttatatcta attgattatc aacacatcta
     7741 atttcgacac aaaggataaa aatcttagat ttttataaat tatgactttt cggagtggac
     7801 tcacagttat aatatatact gtcagagttt ctttttaatt ctatcggaaa tttttcatac
     7861 cggcttattc gagtactgta acccatccgt gtgtgtcggg ttcgtcgccg tactgaatgg
     7921 ccctcagttt gttgtagagc ttagtgcacc acggtcctgg ttttccgtcg ttggagaata
     7981 tgtacgattt attttccaac aagtcgtcga tgcgttcgat ggggctgata acggctgcag
     8041 tgccgcaagc gccggcttct tcgaatgtag cgagttcttc ttcgggcact tcgcgctgtt
     8101 caactttcat ccccatttca gcagccagca ccatcagact tttgttggtg atggatggca
     8161 gtatcgaaga ggatttcggt gtgatgtaag tgtcgttttt gattccgaaa aaattagctg
     8221 caccgcattc gtcgatgtac tttttttctt tggcgtcgag gtagaatact gcagaatagc
     8281 ccatatcatg agctttcatg cctgctacca tgctggcagc ataattgccc ccgattttga
     8341 aagtgccggt tccgagcggg gctgcacggt cgtattcgcg cataataacg tagggtgtgg
     8401 tggcaaaacc tcctttgaaa tagggtccta cgggtgttac aaatatcaga aattcgtatt
     8461 ctttggcagg tcttacaccc acctgggcgc ttgtgcctat gagtaacgga cggatataga
     8521 gagctgcacc gctttcgtag ggtggtatga aacgttcgtt gagtttcacc actttcagta
     8581 cggcttcttc gaatttttcg acaggaagtt ttgccatcat tattccgtcg catgatgatt
     8641 gcagacgttt ggcgttctcg ttcatcctga aaattcgtat ttttccgtct tttcctctga
     8701 aggctttgag tccttcaaat gcttcctgac cgtagtgcag acttgtcgca gccatgtgca
     8761 tggttattgt ctcttctgta caggtcctga tttctcccca ttcgccgtct ttgtatgagc
     8821 aacgaacgtt gtaatcggtt ttcatataac cgaatcctaa attgctccaa tctatgtttt
     8881 ccataataga ataagtaatt agccccctaa ccccctgaag ggggaatata agagcgttta
     8941 taaagttagt tttatatatt tttttattta atttgtatct caatgtttca
//
LOCUS       DAORKP010000095         8920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1135_length_8920_cov_10.579244, whole genome shotgun sequence.
ACCESSION   DAORKP010000095 DAORKP010000000
VERSION     DAORKP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_8920_cov_10.579244"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLN75_06455"
     CDS             complement(<1..328)
                     /locus_tag="PLN75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRG03792.1"
                     /translation="MKRRFLILYFLIAFASIASNTYSTTKSFVFEPFSLNDKLSSNSV
                     TRTFQDRDGYVWFGTKDGLCRFDGYDIKVFRSSALTPGKLTNNEIECLAEDNSGKLWI
                     GTMEGIN"
     gene            691..778
                     /locus_tag="PLN75_06460"
     tRNA            691..778
                     /locus_tag="PLN75_06460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Ser,seq:gga)
     gene            complement(901..1671)
                     /locus_tag="PLN75_06465"
     CDS             complement(901..1671)
                     /locus_tag="PLN75_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRG03793.1"
                     /translation="MIDTHSHIYSEEFDADRDEVILRAQHAGVTKIILPNVDSDSLPR
                     LLKLEARYPDYCYAAVGLHPESVKENYQKELDVIREELERRRYIAVGEIGIDLYWDKT
                     YQTEQILAFQTQVEWAIEFNLPVIIHVRNSHNETIQALKPYAGKGLKGIFHCFGGTPE
                     EAAEIFSLGDFKLGIGGVVTFKNSGLAASLMHIPPENLVLETDSPYLTPAPFRGKRNE
                     SAYTVLVAEKLAQVYDMSVGEIDRITTENACSVFSGLK"
     gene            complement(1767..3284)
                     /gene="guaA"
                     /locus_tag="PLN75_06470"
     CDS             complement(1767..3284)
                     /gene="guaA"
                     /locus_tag="PLN75_06470"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRG03794.1"
                     /translation="MEKIIILDFGSQTTQLIGRRLRELNQYCEILPYNKFPDDTSDVK
                     GVILSGSPYSVYDPSAFKTDLTALRGKLPLLGICYGAQLLAYTAGGTVEAAGSREYGR
                     AILSGIREQDELFKGIHLNSQIWMSHGDSITALPENFSVIANTEDVEIAAFRVQGEKT
                     WGVQFHPEVYHTADGMQLLENFLNICGTERGWSPASFIQSTVNELKETIGKDKVVLGL
                     SGGVDSSVTAVLLNKAIGKNLTCIFVDHGLLRKNEFETVLKDYEHLGLNVIGVDAKEY
                     FYKALRGESDPERKRKIIGAGFIDVFDAEAHKLKDVKWLAQGTIYPDVIESLSITGTV
                     IKSHHNVGGLPEKMHLKLCEPLRLLFKDEVRRVGYELGMMSHLLKRHPFPGPGLGVRI
                     LGEVTPDKVRILQDADDIFINALKDWNLYDKVWQAGVILLPVQSVGVMGDERTYSNAV
                     ALRAVTSVDAMTADWAQLPYDFLAKVSNDIINKVKGVNRVVYDVSSKPPATIEWE"
     gene            complement(3370..4596)
                     /locus_tag="PLN75_06475"
     CDS             complement(3370..4596)
                     /locus_tag="PLN75_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRG03795.1"
                     /translation="MNHPERKEAIYIDNFNYELPDQRIAKYPLAKRDASKLLLWKNGE
                     ISESQFSEIAQFLPENSLLVCNNTRVIQARIVFHKQTGARIEVFCLEPLQPSDYALSL
                     SATGSCEWKCLVGNLKKWKEGTLQQSILMGEKEVIFSAELLETEGNTHRIRFGWNDHE
                     FTFSEILMQIGELPIPPYLHRKTEDADKKTYQTVYSKIEGSVAAPTAGLHFTPEVFES
                     LKFKNIEIEELTLHVGAGTFQPVKSASIHEHTMHTETIAVPLETIKHIQRKIGNIIAV
                     GTTSVRTLESLYYIGKNLPNLPDENFIDFRVDQWEPYEKTTGIPVEKALQNIIDYLEK
                     HDQDTLHAETQILIKPGFQFRVINGMITNFHQPKSTLLLLVSAYVDGGWRKIYDYALA
                     NDFRFLSYGDSSLLLK"
     gene            4682..5752
                     /locus_tag="PLN75_06480"
     CDS             4682..5752
                     /locus_tag="PLN75_06480"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HRG03796.1"
                     /translation="MIKKGDTVRFLNAVGGGKVTRIDEKQNIVYVEDADGFEVPALAR
                     ECVVIEAVNQKTNFPVKDFKSKPVENTMKPTNDVKSPEQSKTPEPIVETPDGELFKSL
                     LAFFATDIKRMETTAYECYLVNDSNYFLYYNFVNGEESNRKSIANGTIEPNMQEFLCN
                     IEKTQLNEWEKVRVQIIPFKKDKIYTEQDALDFMLKINTVKFYKLHSFTETDYFDDPC
                     MLIDLTEERNHSRLREVSPEEIRQVIHQKSQPPLRPRILNKLPQKEIIEVDLHINTLL
                     DSTAGMTNAEMLSYQMDTFHRILEENKNKKGQKIVFIHGKGEGVLRTEIEKQLKSRYK
                     GYLFQDASFREYGFGATMVIIR"
     gene            complement(5826..>8920)
                     /locus_tag="PLN75_06485"
     CDS             complement(5826..>8920)
                     /locus_tag="PLN75_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRG03797.1"
                     /translation="MVTIQGKLSLISYFAKVQVKNEGGSITALPNGGISIQNADAVTI
                     ILAGKTNFSTTSPTYIHDAARVPVDVENIVSKAIAKDYATLKSTHIADYKSLFDRVSL
                     KIGTTSNTVPTNQLITKYASDKDNFLNELYFQFGRYLMISSARGIALPSNLQGIWNNS
                     NTPPWSSDIHSNINVQENYWPAEVTNLSELHNTFLDYIYRESQQRNQWKQNAKDSGQT
                     KGWTLYTENNIFGWHGSFMHNYVIANAWYAMHIWQHYRYTMDKDYLLNKAYPVMKSCA
                     EYWMERLINDKGNATYGFAADGTLVCPNEYSPEHGPSEDGVAHAQQLVWDLFKNTLEA
                     MDSLGTAVAGDAAFRANLQAKFAKLDNGCHTEKDGGKYFLREWKYTPRSVVGDYSHRH
                     ISHLVGLYPGNQISPLINDSIFKAALVALDSRGDYSTGWAMGHRITCWARALDGNRAY
                     RILNNALALSSGGGGIYSNLFDAHPPFQIDGNFAATAAIAEFLIQSHIQGIIQLLPAL
                     PDAWTEGQVKGLRAIGNFTVDMSWVNNLLTTATITSVSGQVCKINFPNAKNATITNLA
                     TGFRVSVMVLDDNTISFPTTANGQYKVTPGPTCEMPVFNPTGGKYTTDLMVTITSATE
                     GAEIYYTLDGTNPTTASTRYSGLITLSKSLTMKAIAAKEGFAVSKVASADYILGDYTV
                     NTPVNAPMTRTDRHLDVVRFTGNSGGSLTGTLPTTGTRLLYTDLTKSVTGYALPGETL
                     TPSMTYTGSWMHGYVYLDKGNDGVFSSTVNVDGSPSAESDVVSYSYYSGKNSTGATVS
                     NQNPGVNSPAFTIPTDMKPGFYRMRYKVDWDYIDAGGNATNTNNIIANGGGIADVLLN
                     VHKTTSTIKINKTNGDVLKSDNTPFSDTEVPFASAVDVVLSPNQGYKQNGMLIKHGYL
                     SNPKFIHGNRQWQIDTIPATQFTDNRFTIPARLIDGDVEITAEFVLLSGVEKPTYDPN
                     LIINTNKNYLTVVAILPAQVKVMDVTGRTHFLGQLSGVRTFKLNSGVYFVNRQKVIVP
                     "
BASE COUNT         2488 a         1929 c         1896 g         2607 t
ORIGIN      
        1 tgttgatgcc ctccattgta ccaatccaca acttgccgct gttgtcttcg gctaagcatt
       61 ctatttcatt gttggtcagt tttcccggtg tcagggcact tgatctgaac actttaatat
      121 catatccgtc aaaacgacat aatccgtctt ttgtgccgaa ccacacgtat ccatcgcggt
      181 cctgaaaagt gcgtgttact gaatttgacg ataatttatc attcagtgag aacggttcaa
      241 aaacaaatga ctttgttgtt gaatatgtat ttgaggctat tgaagcaaaa gctatcagaa
      301 agtataatat taaaaatctt cttttcatca ttgcgaaaat actacattta cttctatctg
      361 gcaaaaatat tgatgaataa taaatgacaa gcatattttc cataaatact gagctgtact
      421 ccgaaatttc actatccggt tattcaattt atatatgctt acttatgaaa cggataaaaa
      481 atcttcccgt ttgcctgatt taattaatat cacagttgaa ttcttccgtc aactcgctct
      541 tctaagaaat ttttcatgga attatcctac cgaatcactt ctaagaatac gccgaagcat
      601 cactttctat gaatcaatgt aaacattatt cagccgttgt gttgcaaata agtatttttt
      661 tgtatctttg ctgtccgttt tcaccctgaa ggagagatgc tcgagtggtt gaagaggcac
      721 gcctggaaag cgtgtatacc cctaaagggt atcgcgggtt cgaatcccgc tttctccgct
      781 gaatatgctg taaatcaacc atctataagt cttacacact gatttacgca cctaaaaagc
      841 aataaaaaag cgggtttttt agcccgctct ttttatttta aaagtgattt tcctgttttg
      901 ttatttcaga cctgaaaaaa ccgaacaggc attttcagta gtaatccggt caatttctcc
      961 tacgctcata tcatagactt gtgccagttt ttcggctacc aaaaccgtgt aagccgattc
     1021 gtttcgcttt ccgcgaaacg gtgccggtgt aaggtaaggg gagtcggtct caagtaccag
     1081 attttcgggc ggaatatgca tcaggctggc agccaggccg gagtttttga acgtcaccac
     1141 cccacctatg cccagcttga aatcacccag actgaatatc tcggcagctt cttccggagt
     1201 tcctccaaaa caatggaaaa tcccttttaa gcccttgcct gcgtatggtt tgagtgcctg
     1261 aattgtttca ttatgcgaat tacgcacatg aataatgaca ggcaggttga actcaatggc
     1321 ccattccacc tgagtctgaa aagcaaggat ttgttcagtc tgatatgttt tatcccagta
     1381 gagatcaatg ccaatttcgc ctacggcaat atatcttctt cgttcgagtt cctctctgat
     1441 tacgtccagt tctttttgat aattctcttt cacactctcg ggatgcaagc caacagcagc
     1501 ataacaatag tcaggatatc ttgcttccag tttcagcaag cgaggcagag aatcggaatc
     1561 gacattggga agaattattt tggtcacccc ggcatgttgc gcacgcagga ttacctcatc
     1621 gcgatcggca tcaaattctt cggaatagat atgtgaatgg gtgtcaatca taaggcccgc
     1681 taaatccacc caaagggaga tttttggtat gatttattaa taaaaaatat atttggcatc
     1741 ccttcaggga ggtttggtgg ggctttttac tcccactcga tggttgcggg cggtttcgaa
     1801 ctgacgtcgt acaccacgcg gttcacgcct ttcactttat tgataatatc gttggatacc
     1861 ttggccagaa aatcgtaggg gagctgtgcc cagtcggcag tcatggcatc taccgaagtg
     1921 acagcacgca gcgctacggc attgctgtag gtacgttcgt cgcccataac tcccacagat
     1981 tgtaccggga gtaatatgac accggcctgc cagactttat cgtacagatt ccagtctttc
     2041 agtgcattta taaaaatatc gtcggcatcc tgcagaatac gtactttatc gggggtgact
     2101 tctcccagaa tgcgtactcc cagtcccggg ccggggaaag gatgacgttt gagcaggtgg
     2161 ctcatcattc ccaactcata ccctactctg cgtacttcgt ccttaaagag caaccgcaac
     2221 ggttcgcaga gttttaagtg catcttttcg ggcagtccgc ctacgttgtg atgcgatttg
     2281 atgactgtac ccgtgatgga gagcgattca attacatcgg gataaatagt gccctgtgcg
     2341 agccatttta catctttcag tttgtgtgct tcggcatcaa atacatcaat aaatcctgca
     2401 cctattattt tgcgtttacg ctccggatct gattctcccc ggagtgcttt gtagaaatat
     2461 tctttggcat caactcctat cacattaagt ccgagatgtt cataatcttt cagtacggtt
     2521 tcaaattcat tttttcgcaa caatccgtga tccacaaaaa tgcaggtgag attttttccg
     2581 attgccttat tgagcaaaac tgctgtaacg gatgaatcca cccctcctga tagtccgagc
     2641 accactttat ctttgccgat agtttctttt agttcgttga cagtggattg aataaaagaa
     2701 gcgggtgacc atcctctttc ggtgccgcaa atattaagaa agttctccag aagctgcata
     2761 ccatcagccg tatgataaac ttcggggtga aactgaactc cccatgtttt ttcaccttgt
     2821 acccggaaag cggcaatctc tacatcctcg gtgttagcaa tcacactgaa attttccggc
     2881 agagcggtga tggagtctcc atgcgacatc cagatttgcg aattcaggtg aattcctttg
     2941 aagagttcat cctgctcccg gatacccgat aagatagctc tgccatactc gcggcttccg
     3001 gctgcttcca cggttccgcc ggcggtgtaa gcaagaagct gagctccgta gcagatgccg
     3061 agtagaggca gtttacctct cagagcagta agatcagttt taaaggccga aggatcatat
     3121 acagaatatg gacttccgga aagtatcacc cccttcacat cggatgtatc atcggggaat
     3181 ttattgtaag gcagaatttc acaatattgg ttcagctcgc gcagacggcg tccgataagc
     3241 tgtgtggtct gtgaaccaaa atcgaggatg ataatttttt ccatggagaa tttgatctgt
     3301 tttaatgttt tgcaaaaata gcaaaaaagt aagaaatgag gaaatgaaat ccgattaatc
     3361 ggtttctact tatttcagca acagcgaact gtcgccgtag ctcagaaagc gaaaatcatt
     3421 tgcgagggcg taatcgtata ttttgcgcca accgccatcc acgtatgcag atacgagaag
     3481 caatagggta cttttcggtt gatgaaagtt ggtaatcatt ccattgataa cgcgaaactg
     3541 aaaacccggc ttaataagaa tttgtgtttc ggcatgcagc gtgtcctggt catgtttttc
     3601 cagataatca ataatgtttt gaagtgcctt ttctacagga attccggttg ttttttcata
     3661 cggctcccac tgatcaaccc tgaagtctat aaagttttca tcgggtaaat tcgggagatt
     3721 ttttccgata tagtacaaac tttccaaagt acgcaccgaa gttgtaccaa ccgcaataat
     3781 attcccgatt tttctctgaa tatgtttaat cgtttccagc ggcactgcaa tggtttcggt
     3841 gtgcattgta tgttcatgga tggaggcaga ctttaccggt tggaatgtac cggctcccac
     3901 atgtagggta agttcttcaa tttcaatgtt tttaaatttc agactttcaa acacttccgg
     3961 tgtgaaatgc agtccggcag tgggtgccgc caccgagcct tcaattttgg aataaaccgt
     4021 ttgataggtt tttttatcgg catcttccgt tttgcggtgc aaataaggag gaatgggaag
     4081 ttcgcctatt tgcatcagga tttcagaaaa ggtgaattcg tggtcgttcc agccgaagcg
     4141 tattcggtga gtatttccct cagtttcgag tagctcggcc gaaaaaatca cctctttttc
     4201 acccatcagt attgactgct gtaaagttcc ctctttccat tttttaagat ttccaacgag
     4261 gcatttccac tcgcaactgc ctgtagccga taaggaaagt gcataatccg atggctgcag
     4321 gggttccagg caaaacacct cgatccgggc acccgtctgc ttgtgaaaaa caattctggc
     4381 ctgaattaca cgggtattgt tgcaaaccag cagtgaattt tcaggtaaaa attgagcaat
     4441 ttcggagaat tgactttcag aaatctcgcc atttttccaa aggagcaatt tggaggcatc
     4501 gcgttttgca agcggatatt ttgcaatccg ctggtcgggt aattcgtagt taaaattgtc
     4561 aatgtaaatg gcttcttttc tttcgggatg gttcacgagg caataaaagt ttgtaaattt
     4621 gcgcaaaatt aagacaatta gaaccaagcg acaagctatt tgacataaaa aaacaaagac
     4681 tatgataaaa aaaggtgata cagtgcgttt tctgaacgca gtaggtggcg gcaaagtgac
     4741 ccgcatcgat gaaaaacaaa atatagtata tgtggaagat gccgacggat ttgaagttcc
     4801 ggcacttgcc cgtgaatgtg tggttattga ggcagtaaac cagaaaacga atttcccggt
     4861 taaagatttc aaatcaaaac cagtggaaaa cacaatgaaa ccgacgaatg atgtgaaatc
     4921 gcctgaacag tcgaaaactc ccgaaccaat agtagaaact cctgatggtg agctatttaa
     4981 atcactcctg gcgtttttcg caactgatat aaaacggatg gagacaacag cgtatgagtg
     5041 ttatttggtg aacgacagca attattttct gtattacaat ttcgtaaacg gcgaagaaag
     5101 caaccggaaa agcattgcaa acggaaccat tgaacccaac atgcaggaat ttctgtgcaa
     5161 cattgaaaaa acgcaactga acgagtggga aaaggttcgc gtgcagataa tcccgttcaa
     5221 aaaggacaaa atatatacgg aacaagacgc gctcgatttc atgcttaaaa tcaatacggt
     5281 gaagttctat aagctccaca gttttaccga aacagattat ttcgacgatc cgtgtatgct
     5341 aatcgatctg actgaagaaa gaaatcacag ccgcctgcgg gaggtttcgc ccgaagaaat
     5401 acgtcaggta atacatcaaa aaagtcaacc gccactccgt ccgagaattt tgaacaagct
     5461 gccgcaaaag gaaatcattg aagtggatct ccacatcaac actctgctcg acagtacggc
     5521 tggtatgact aacgccgaaa tgcttagcta tcagatggat acattccacc gcattctgga
     5581 agaaaacaaa aacaagaaag gacagaaaat tgtatttatc catggcaaag gcgaaggagt
     5641 actgcgaacc gagatagaaa aacaactgaa aagccgctat aagggttatc tttttcagga
     5701 tgcttccttc cgtgaatatg gttttggagc tactatggtt attataaggt gataaaatat
     5761 tttacacaaa aagagcagtt cagatttttt ccgaactgct ttttttattt tcatttttat
     5821 aacccttaag gcactatcac cttttgcctg ttgacaaaat acacaccgga atttaattta
     5881 aaagtacgta cacctgataa ctgtccaaga aaatgtgtcc gtccggttac atccatcact
     5941 tttacctgag ccggcaaaat tgcaacaacc gttaaataat ttttattggt attgattatc
     6001 aaattcggat cgtaagtcgg tttttcaact ccggaaagca aaacaaattc tgctgtaatt
     6061 tccacatcac catcgattaa ccttgctggg attgtgaacc tgttatcggt aaactgagtt
     6121 gctggtatcg tatcaatctg ccattgcctg ttgccgtgta taaacttcgg atttgaaaga
     6181 tacccgtgtt taatcagcat tccattttgc ttataaccct gatttgggct taaaaccaca
     6241 tcaaccgctg aagcaaacgg aacctcagta tctgaaaaag gagtattatc agatttcaga
     6301 acatcaccgt ttgttttgtt tattttgata gttgatgtcg ttttatgtac attgagcaac
     6361 acatcagcaa ttccaccacc gtttgcaatg atattattcg tatttgtggc gttgccgcct
     6421 gcatcaatat aatcccagtc cactttatag cgcatacgat aaaagcccgg cttcatatca
     6481 gtggggatgg taaatgccgg agaatttacg cccggatttt ggtttgaaac agttgctccc
     6541 gtactgtttt taccactata ataagaatac gaaaccacat cagattctgc tgatggagaa
     6601 ccatctacat ttacagtcga tgaaaaaacc ccatcatttc ccttatccag gtacacataa
     6661 ccgtgcatcc aacttccggt gtaggtcatg gagggagtga gtgtttcgcc tggtaaagcg
     6721 taacctgtaa cagatttcgt taaatcagta tataaaagcc tggttcctgt ggtgggcaaa
     6781 gttccggtca ggctacctcc cgagtttcct gtaaaacgaa caacatccag atgacgatcg
     6841 gtacgtgtca ttggagcatt gacgggtgta tttacggtat aatcacccaa aatatagtca
     6901 gcagatgcta ctttacttac agcaaagcct tctttggcag caatcgcttt cattgtcagt
     6961 gattttgaaa gcgtaatgag accggaatat cttgtcgatg ctgttgtagg atttgttcca
     7021 tctaaagtgt aatagatttc agctccttca gttgctgatg tgattgtaac catcaaatcg
     7081 gtggtgtatt ttcctccggt aggattaaaa acaggcattt cgcatgtcgg gcccggtgta
     7141 actttatatt gtccgtttgc tgtggtaggg aaactgatgg tattatcgtc aaggaccata
     7201 acacttaccc gaaaaccagt agccaggttt gtaattgttg cattttttgc attcgggaaa
     7261 ttgattttac atacctgtcc gctgacagat gtaatcgtag cagtagtcag caaattgttc
     7321 acccagctca tatccactgt gaaattaccg attgctctca aaccttttac ttgtccttca
     7381 gtccatgcat cgggcaatgc gggcagtaac tgtattattc cctgaatatg gctttgaatc
     7441 aagaactcgg caatagcagc agtagccgca aagtttccgt caatctggaa aggcggatga
     7501 gcatcgaata gattggaata gatacctccg ccaccggatg ataaagcaag agcattattc
     7561 aatatccggt atgctctgtt accatctaat gcgcgtgccc aacaagtgat gcgatgtccc
     7621 attgcccaac cggtgctgta gtcgccgcgc gaatcaagcg caacaagggc ggctttgaat
     7681 atggagtcat taattagtgg cgaaatctga ttgccggggt ataatcccac cagatgcgaa
     7741 atatgccggt ggctataatc acctactaca gaacggggtg tatatttcca ctcacgcagg
     7801 aagtattttc caccgtcctt ttcagtatga cagccattat cgagcttagc aaatttcgcc
     7861 tgaaggtttg cacgaaaggc tgcgtcacct gccactgctg tacccagtga atccatggct
     7921 tccagcgtat ttttgaacaa gtcccaaacg agctgctgtg catgcgccac accatcttcc
     7981 gaaggtccgt gttcgggcga atactcgttc ggacaaacca gtgttccgtc agcagcaaaa
     8041 ccataagtag cgttcccttt gtcattgatt aatctttcca tccagtattc ggcacaggat
     8101 ttcatgaccg gataagcttt gttgagcaga taatccttat ccatggtgta gcgatagtgt
     8161 tgccagatgt gcatggcata ccaggcatta gcaattacgt agttatgcat aaaactaccg
     8221 tgccatccga agatattgtt ttcggtgtag agtgtccatc ctttggtttg tcccgaatct
     8281 ttcgcatttt gcttccactg gttacgttgc tgcgactctc ggtagatata atccagaaag
     8341 gtattgtgta attccgaaag gtttgttact tcagccggcc agtaattttc ctgtacatta
     8401 atattactgt gaatatcact gctccagggt ggcgtgttgc tgttattcca gataccctga
     8461 agattagaag gaagagcaat tccccgggca gaagaaatca tcaggtatcg gccaaactgg
     8521 aaataaagtt cattcaggaa gttatctttg tctgaagcgt attttgtaat taactggtta
     8581 gtaggcaccg tatttgaagt agtgccaatt ttgagcgaaa cgcggtcaaa taatgattta
     8641 tagtcagcaa tatgcgtgga tttcaaggtt gcataatctt tcgcgattgc tttgctgaca
     8701 atattttcta catctacagg aactcttgca gcatcatgaa tgtaggtggg acttgtggtc
     8761 gagaaattgg tttttccggc cagaataatc gttactgcat ctgcattttg aatacttatt
     8821 ccaccattgg gcaatgcggt aattgatccg ccttcatttt taacctgaac tttggcgaag
     8881 tacgaaatca gacttaactt tccctggata gttaccatat
//
LOCUS       DAORKP010000096         8861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1150_length_8861_cov_8.544515, whole genome shotgun sequence.
ACCESSION   DAORKP010000096 DAORKP010000000
VERSION     DAORKP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_8861_cov_8.544515"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /gene="infB"
                     /locus_tag="PLN75_06490"
     CDS             <1..2562
                     /gene="infB"
                     /locus_tag="PLN75_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRG03798.1"
                     /translation="PKPEEKPIVKEPEKPVAKAEETKTEPKQEKTQKSEIKPEEKTEV
                     KAETGTKPAKEIKQETKPEKTEEFKPKPELTETEIKVQPEPEKVAVKDTKPQETAEPE
                     DKQNEDEVYRIGKPKLTNSPVVVGSIDLDSLNQSTRPKSKTKEQKRKEREERNKASVE
                     IKTKKTEPVKASDATAANKQSKRKRINKQHVDLSKPENFDTGKKPAKKEHFKKPIKTV
                     VNEEDVQKQIKETLARLSQANNKRGKGAKYRRDKRESISAKLHEQEALERSEEKLLKL
                     TEFVTVSELATMMNVSVNEVIGTCMAIGMMVSINQRLDAETINIVADEFGFKTEYVSH
                     DVVEALAEEEDNEEDLVSRAPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIG
                     AYNVKLDNGQRITFLDTPGHEAFTAMRARGAKVTDMAIIIVAADDNVMPQTIEAINHA
                     SAANVPIIFAINKIDKPGANPEKIKETLANMNYLVEDWGGKYQSQDISAKQGIGVSEL
                     LEKVLLEAELLELKANPKRRAIGSIIESSLDKGRGYVATVLVENGTLHQGDIVLAGIY
                     QGKIKAMFNERNQRIEAAKPSEPVLILGLNGAPQAGDTFNVVDSEQEAKEITNKREQL
                     QREQSIRTKKHFTLDELGRRIALGDFKELNIIVKGDVDGSIEALADSLLKLSTEHIQV
                     NVIHKAVGAISDSDILLAAASNAIIVGFQVRPSVSARRMAEKEEIDIRLYSIIYDAIE
                     EIKSAMEGMLSPEIKKEVTATIEVLEVFKISKVGTVAGCIVREGKAKRSNKLNLIRDG
                     IVVFTGELDAIKRFKEDVKEVGVNFECGISIKNYHDIRTGDIIETFEEIEVKQTL"
     gene            2721..3407
                     /locus_tag="PLN75_06495"
     CDS             2721..3407
                     /locus_tag="PLN75_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRG03799.1"
                     /translation="MLIKSTNILFIALLLVLISGNRMNAQGNLPFADEKKIHYGFSLG
                     MNFMDFAVVPASTAHDASVTNIIPGFSVGAITDLRLTRYLNLRFTPTLMLSQRILNYS
                     DSSSTDLLSVPLYLPLYLKYSSVRTGNFRPYLLAGGGLWIDWGRNKERTVLLKPFDVL
                     MEAGVGCNIYFSFFKLAPELKFSFGFNNMLTPLDERDAGALLNPQTIRHTESISKLTT
                     RMITLTFNFE"
     gene            3467..4108
                     /locus_tag="PLN75_06500"
     CDS             3467..4108
                     /locus_tag="PLN75_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRG03800.1"
                     /translation="MKKAIVIGSTGLVGTALVSQLLENDNYSEVISIVRRKSDSAHPK
                     LTEHIVDFDKPDLWKNLVKGDVLFSAMGTTIKAAKTKDNQYKVDYTYQFETAKAACES
                     GVPVYVLVSSAGASSSSLNFYTNMKGKLEDSVKQLPFKTIQILQPGQLDGNRKEHRTG
                     EKMALKMMHFFNKMGFLMKYRPIQGSEVARFMIMIAEKPVSGVYKLDELFKIK"
     gene            complement(4163..4804)
                     /locus_tag="PLN75_06505"
     CDS             complement(4163..4804)
                     /locus_tag="PLN75_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRG03801.1"
                     /translation="MQIKTILFDFDGVVVDSESLHLRALGEVLTNHGISYPDHLLEKF
                     VGKSDSSFYKYVIDNLNSSFELDYLLDQKNKIFEQIIHELQPIDGFLDFIDIVIRKNI
                     PRAIVTSSSNETLKMVSEIFPFQNYFDIVVCEEDTTEHKPKAAPYLLGLERTGGEKET
                     TLVIEDSVNGIKAGKSAGCIVFGLTTSLPREALIEAGADRVFDSYEEIREKIW"
     gene            complement(4953..6626)
                     /gene="ettA"
                     /locus_tag="PLN75_06510"
     CDS             complement(4953..6626)
                     /gene="ettA"
                     /locus_tag="PLN75_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRG03802.1"
                     /translation="MADDKKIIFSMVGVSKTFPPQKRVLNNIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGVEKNFDGNVVFSPGYSVGYLEQEPQLDDTKTVKQIVQEGTQEVVD
                     MLAEYDRINEQFAEPDADFDKLIARQGELQELLDHADAWNLDNKLDRAMDALRCPPED
                     ALVKNLSGGERRRVALCRLLLRQPDILLLDEPTNHLDAESVEWLENHLHDYAGTVIAI
                     THDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTTRMAMEEKQASKRRKTLEREL
                     EWVRMAPKARQAKGKARLEAYDRLLNEDQKEREEKLELFIPNGPRLGNKVVEAIAVSK
                     AFGEKLLFENLNFMLPPNGIVGIIGPNGAGKTTLFRMMMGMEKADKGTFDVGETVKIG
                     YVDQSHADIDPKKSVYQVISGGTEFIRVAGKDINARAYLSRFNFTGADQEKLCGVLSG
                     GERNRLHLALTLKSEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHDRWFLD
                     RICTHIMAFEGDSQVFVFEGSYSEYEENKKMRLGDVEPKRIRYKKLLAP"
     gene            6853..7251
                     /locus_tag="PLN75_06515"
     CDS             6853..7251
                     /locus_tag="PLN75_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRG03803.1"
                     /translation="MKKLFFIIIGSLCLILGITGIFLPVLPTTPFLLLSAALFARSSD
                     RLYLWLINHKIFGEYIRNFREDKAITLQGKIISISTLWLFMLYSIFYIVSEKWYLQVL
                     LGSIALGVSVYILSFKTKVSAGKNKDYTED"
     gene            complement(7236..8387)
                     /locus_tag="PLN75_06520"
     CDS             complement(7236..8387)
                     /locus_tag="PLN75_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03804.1"
                     /translation="MKLLKQQIRESFFNPILYFLPALIFMVADDFWGENTAWKVSFPI
                     AFAMMFYIYYMYKGMFIWHGMLAVCYLIIGFTSSLVPDTHPVLIYIDELTFILFILLM
                     ILQKKSLGKIASKALPYKLPMFNNENELFRVARVLMLIVIAFVFFSLYFRIFDIHKKE
                     IPLGYLKYLYASTLIFAALFELIRVSIIRARLIREDWVPVVDESGRVTGSVQYRRDHP
                     KEERLMHPQVRLYFLENGMIYLQQRKADDINEPLLWDASLSRSVRMTESIDLVLNKYT
                     KKLYNQDQQKFLFLTNYIYNGRFNDQYIYLFISCKTDELKPQKDEVFQTKWWTTRQIE
                     DNLGKNIFTERFEKEYDILKRSGLLEQESCECECALRELVKNKNGQSSV"
     gene            complement(8553..>8861)
                     /locus_tag="PLN75_06525"
     CDS             complement(8553..>8861)
                     /locus_tag="PLN75_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRG03805.1"
                     /translation="HVDQTAICKNVEEADGSWYFTFEYEFDPVKARKGYMTVDKGSVT
                     VNGVSLTVVEPTNNSFRVAIIPYTYEYTNFHQIKVGTTVNIEFDIIGKYISRMLEFNP
                     "
BASE COUNT         2729 a         1960 c         1832 g         2340 t
ORIGIN      
        1 ccaaaacccg aagaaaaacc aattgtaaaa gagcccgaaa aacccgttgc gaaagctgaa
       61 gagactaaaa ctgaaccaaa acaggaaaaa acccaaaaat ccgaaatcaa accagaagaa
      121 aagactgaag taaaagcgga aaccggtaca aaacctgcaa aggaaataaa gcaggaaacg
      181 aaacctgaaa aaactgaaga attcaaacca aaacccgagt taaccgaaac ggagataaaa
      241 gtacaaccgg aacctgaaaa agtagctgta aaggacacca aaccgcaaga aaccgctgaa
      301 ccggaagaca agcagaatga agacgaagtt tacagaattg gaaaaccaaa acttacaaac
      361 tcaccggttg tagtaggttc tattgatctg gacagcctca atcagagcac aagacccaag
      421 tcaaagacca aagagcagaa acgtaaagaa cgtgaagagc gaaataaggc ttcagttgaa
      481 atcaagacta aaaaaactga acctgtaaaa gcatccgacg caactgctgc aaacaaacag
      541 agtaaacgca aaagaataaa caaacaacac gtagacctga gcaaacccga aaacttcgac
      601 accggaaaaa aacccgcaaa gaaagaacac ttcaaaaaac cgattaaaac agtcgtaaac
      661 gaagaggatg ttcagaaaca gattaaggaa actctcgcca gactttcgca ggctaacaac
      721 aaacggggaa aaggagccaa atatcgccgc gataaacgcg aaagcataag cgccaaactt
      781 catgaacagg aagcactgga gcgaagcgaa gaaaaattgc tgaaactcac cgaatttgtt
      841 accgtaagcg aactggctac catgatgaac gtatcggtga acgaagtgat aggtacctgt
      901 atggccatag gcatgatggt atccatcaat cagcgcctgg atgccgaaac cattaacatc
      961 gtagcagatg aattcggatt caaaacagaa tatgtgagtc atgatgtggt tgaagccctt
     1021 gctgaagaag aagacaacga agaagatctg gtttcacgcg ccccgattgt gacagtcatg
     1081 ggtcacgttg accacggtaa aacttcactg ctcgactata tacgtaaaac aaacgtgata
     1141 gccggtgaag ccggaggaat cacacaacac attggtgcat ataatgttaa actggacaac
     1201 ggacagcgaa tcacattcct tgacacaccc ggtcacgagg catttaccgc catgcgtgca
     1261 cgtggtgcca aagttaccga tatggcaatc atcattgtag cagccgacga taacgtgatg
     1321 cctcaaacca ttgaagcaat aaaccatgcc tcggcagcca atgtaccgat tatttttgca
     1381 atcaacaaaa ttgacaaacc gggcgcaaat cccgagaaga taaaagaaac gctggcaaat
     1441 atgaactacc tggtagaaga ctggggcgga aaatatcagt cacaggacat atcggccaag
     1501 cagggaattg gagtaagcga actgctcgaa aaagtactcc tcgaagccga gctgctcgaa
     1561 ctgaaagcaa atccgaaacg acgcgccatc ggttcgatca ttgaatcatc gcttgataag
     1621 ggacgcggat atgtagccac agtacttgtg gaaaacggaa ctcttcatca gggtgatatt
     1681 gttttggccg gtatttatca gggcaaaatt aaagccatgt tcaatgaaag aaatcagcgc
     1741 attgaagctg caaaaccttc cgaaccggtt cttattctcg gattgaacgg agcaccacag
     1801 gctggcgata cattcaatgt ggtagacagc gaacaggaag caaaagaaat aaccaacaaa
     1861 cgcgaacaac tgcagcgtga acaaagtatc cgcactaaaa agcactttac actcgatgaa
     1921 ctcggacgac gaatagcact gggcgatttc aaagaactga atatcattgt aaaaggcgac
     1981 gtggacggat caatcgaagc acttgccgat tcattactca aactttccac agaacacata
     2041 caggttaacg ttattcacaa agctgtgggt gctatttcgg actctgatat acttctggct
     2101 gcagcatcca acgctatcat cgtcggattc caggtacgtc cttctgtttc tgcccgtaga
     2161 atggccgaaa aagaagaaat agatatccgc ctgtattcca tcatttacga tgcaatcgaa
     2221 gaaataaaat cggccatgga aggaatgctt tcgcccgaaa taaagaaaga agtgaccgcc
     2281 accatcgaag tgcttgaagt attcaaaatt tcaaaagtag gtactgtagc gggttgtatt
     2341 gtgcgcgaag gaaaagcaaa acgctcaaac aaactaaacc tcatccgcga tggaattgta
     2401 gtcttcaccg gtgaactcga cgctatcaaa cgtttcaagg aagacgtgaa agaagtaggt
     2461 gtaaactttg aatgtggtat cagcatcaaa aattatcatg acatacgcac tggcgacatc
     2521 atcgaaacat tcgaagagat tgaagtcaaa caaacacttt aatacaaaat ctcttttcgg
     2581 caaatgaccg gaagatgaaa accgatcgtc atattcttca aatttccatt tcagccgaaa
     2641 tggaaatttg cttttaaata cagacctgat tatactaaaa aagcgtttac gacgtttttc
     2701 aaacaaacag gaaagtcagt atgcttatta aatccaccaa catacttttc attgcacttt
     2761 tgctagtact catttcgggc aaccggatga atgctcaggg caacctgcca tttgccgatg
     2821 aaaaaaagat acattacgga ttctccctgg gtatgaattt catggatttt gcagtcgtac
     2881 cggcatccac ggctcatgat gccagcgtga ccaacattat ccccggattt tcagtaggtg
     2941 caatcaccga tttgcgcctg acacgttact tgaacctgcg cttcactcct actctcatgc
     3001 tcagtcagcg cattctgaat tattccgatt catcaagcac cgatttactg tcagtgccac
     3061 tctatctgcc tctttacctt aaatacagct ctgtaagaac cggtaatttc aggccctacc
     3121 tcctcgcggg cggaggacta tggattgact ggggcagaaa caaagagcga actgtactgc
     3181 ttaaaccatt cgatgtgctg atggaagcag gcgtgggatg caatatttat ttctcgtttt
     3241 tcaaactggc acccgaactg aaattttcat tcggtttcaa caacatgctg actcccctcg
     3301 acgagcgcga cgcaggtgcg ctgctcaacc ctcaaaccat caggcacacc gaatcaatca
     3361 gcaaactcac tacccgcatg attacactca cattcaattt tgaatagatt tttttctccg
     3421 gaatgaaata aataattttg tttcaaaaaa aaaactcagc attaaaatga aaaaagcaat
     3481 tgttataggt tctacaggat tggtaggcac agcattggtc agtcaattgt tggaaaacga
     3541 caattattcg gaagtgattt cgattgtgcg cagaaaaagt gattcagctc atccaaaact
     3601 aaccgaacat atcgtggatt ttgacaaacc cgatttatgg aaaaatcttg taaagggaga
     3661 tgtgctcttt tcggcaatgg gaacaactat taaagcagct aaaaccaagg acaatcagta
     3721 caaagtagat tatacctacc agtttgaaac ggctaaagca gcttgtgaaa gcggtgttcc
     3781 cgtatatgta cttgtctcat cggcaggtgc cagttccagt tcattgaatt tttacaccaa
     3841 catgaaggga aaactggaag attcggtaaa acagcttccg ttcaaaacca tccagatact
     3901 gcagcccggg caactggacg gcaacagaaa ggaacaccgg acaggtgaaa aaatggcgct
     3961 gaaaatgatg cattttttca acaagatggg ttttttgatg aaataccgcc ccattcaggg
     4021 aagcgaagtg gcccggttta tgattatgat agccgaaaaa cctgtttcgg gagtgtataa
     4081 gctggatgaa ctgtttaaga ttaagtagct attggtgaat caactaagtt gcagtcaatt
     4141 gcaattgact gctacttaat ctttaccaaa tcttttctct gatctcctca taactatcaa
     4201 aaaccctatc cgcaccggct tctatcaggg cttcccgcgg taatgaagtt gtcagtccga
     4261 aaacaatgca accggcagat tttccggctt ttataccatt gactgaatct tcgattacga
     4321 gcgtggtttc tttttcgcca cccgtccgct ccaaacccaa caagtacggc gctgcttttg
     4381 gtttatgctc ggtggtatct tcttcacaaa ccacgatatc aaagtaattt tgaaaaggaa
     4441 atatctctga aaccattttc agtgtttcgt tggaggagga cgtcacaatg gctcgcggga
     4501 tgtttttccg gatgacaata tcaatgaaat cgagaaatcc gtcgatgggt tggagttcgt
     4561 gaataatctg ctcgaaaatc ttgtttttct ggtcgagcag gtaatccagt tcaaaactac
     4621 tattcagatt atcaatcaca tacttataaa acgacgagtc acttttaccc acaaactttt
     4681 ccagcaaatg atcgggatag ctgataccgt gattggtcaa cacttctccc aaagccctca
     4741 gatgcagact ttcagagtca acaactactc cgtcaaaatc gaaaaggatg gtttttattt
     4801 gcatatagta gcccgcctca atcccccgaa gggggactta aagatgtgta ttcaagatcg
     4861 tttaattttt tttatatcag acgggaataa aactgcaata aattgtgtat ctctaaatat
     4921 tggcaattac caaaagtccc ccttcggggg atttaagggg ctaataactt cttgtaccta
     4981 atccgtttgg gttccacatc gcccaaacgc atttttttgt tttcttcgta ttccgaataa
     5041 cttccttcga agacaaacac ctgcgaatcg ccttcaaaag ccatgatatg ggtacaaatc
     5101 cggtccagaa accagcggtc gtggctgata accactgcac atccggcaaa attttccagt
     5161 ccctcttcga gagctctcag cgtatttacg tcgatatcgt tggtaggttc atcgagtagc
     5221 agcacgttgg cttcagattt cagtgttaga gccagatgca gccggtttct ctcaccaccg
     5281 gaaagtacac cgcatagctt ttcctgatcg gcgccggtaa agttaaacct cgaaagatag
     5341 gcacgtgcgt tgatatcttt tccggcgacg cggataaatt ctgttccgcc cgaaataacc
     5401 tgatacaccg attttttagg atcgatatca gcgtgtgact ggtccacgta acctattttc
     5461 acagtctccc ccacgtcaaa ggtaccttta tccgcttttt ccattcccat catcatacgg
     5521 aaaagcgtgg tttttccggc tccgttcggg ccgataatgc ccacaatgcc gttgggtggc
     5581 agcatgaagt tgagattttc aaacaatagt ttctcaccga atgcttttga aaccgcaatt
     5641 gcctccacca ctttgttccc caaacgcggt ccgttcggta tgaaaagttc cagtttttct
     5701 tcccgttctt tctggtcttc attcagcaag cggtcgtaag cctccagacg ggctttcccc
     5761 ttagcctgac gggccttggg tgccatgcgt acccattcca actcgcgttc aagtgtttta
     5821 cgtcgtttgc ttgcctgttt ttcctccatt gccatccggg tggttttttg ctccagccac
     5881 gaagagtaat ttcctttcca gggtatgcct tctcccctgt ccagttccag aatccagcct
     5941 gccacattat ccagaaaata acggtcgtgt gtaatggcaa tgaccgttcc ggcataatcg
     6001 tgcaggtgat tttcgagcca ttctaccgat tctgcatcca ggtggttggt tggttcatcg
     6061 aggagaagta tatcgggctg tcggagcagt agccggcata gcgccacacg gcggcgttcg
     6121 ccacccgaaa gatttttcac caacgcatct tcgggcggac agcgaagagc atccatggcg
     6181 cggtcgagtt tattatcgag attccaggca tcggcgtgat cgagcagctc ctgcaattca
     6241 ccctgacggg caatgagttt gtcaaaatcg gcatccggtt cggcaaactg ttcgttgatg
     6301 cggtcatatt cggccagcat gtccaccact tcctgggtac cttcctgtac aatttgcttt
     6361 accgttttag tatcatcgag ctgtggttcc tgttccagat aacctacgga atatccggga
     6421 gagaaaacca cgttgccatc gaaatttttc tcaacacctg caataatttt cagcaaagta
     6481 gattttccgg agccattcag ccctatgatt ccaattttgg caccgtagaa aaacgaaagg
     6541 taaatattat tcagaactcg tttttgaggc ggaaaagttt tggaaactcc caccatggag
     6601 aaaataatct ttttatcgtc agccatattt atttttttaa cagttagatt gttagataat
     6661 agacttttag acggatgacc gaagttgttc gggtgttcgg ttgttcggat gtttaggtgt
     6721 tattgagcta aacaatcaaa ccaatgaaca aataaacttc aagcttcgtg ttaccaactc
     6781 caagctattt ttggcaaaga tacgagaaaa aggcacaata aacaacgtga ttttattacc
     6841 tttgcatcct gtatgaaaaa attgtttttt atcataatcg gttcactttg tctgatcctc
     6901 ggaattacag gaatttttct tcccgttttg cctaccactc cttttctttt gctttctgct
     6961 gctttgtttg cacgcagttc ggacaggttg tacctgtggc ttatcaatca taaaattttc
     7021 ggtgagtaca ttcgtaattt cagggaagat aaagccatca cgcttcaggg aaagattatt
     7081 tccatctcca cactctggtt gtttatgctt tacagcattt tttatattgt gagtgagaaa
     7141 tggtatcttc aggttttact ggggagtatt gctttgggag tttcggtgta tattctttcc
     7201 tttaaaacga aagtgagtgc cggaaaaaac aaggattata ctgaggactg accatttttg
     7261 tttttcacca actctctgag agcacactcg cactcacagg attcctgttc gagtaatccg
     7321 cttcgtttga ggatatcata ttctttttca aatcgttcgg tgaagatatt ctttccaaga
     7381 ttatcttcta tttgtctggt agtccaccat tttgtctgaa atacttcatc tttttgaggt
     7441 ttcagttcat cggttttgca cgagataaac agataaatat attgatcgtt gaacctgccg
     7501 ttgtaaatgt aatttgtcag aaaaagaaat ttttgctggt cctgattata caactttttg
     7561 gtatatttat tcagtaccaa atcaatagat tcggtcatac gcacactccg gctgagtgag
     7621 gcatcccaga gcaacggctc gttgatgtca tcggcttttc gttgctgcaa ataaatcatt
     7681 ccgttttcaa ggaagtatag ccttacctga ggatgcatca gcctttcttc ttttggatga
     7741 tcgcgcctat attgcacact ccctgtaact ctacccgatt catccactac cggtacccag
     7801 tcttcacgga taagcctcgc tctgatgatg cttacacgaa ttaattcaaa caaagcggca
     7861 aatataagtg tgctggcata caagtatttc aggtatccga ggggtatctc ttttttatga
     7921 atatcaaaaa tgcgaaaata gagtgaaaaa aacacaaacg ctatcacaat taacatcaac
     7981 acccgggcaa cgcggaaaag ttcgttttcg ttgttaaaca tcggcagttt gtaaggaagt
     8041 gctttggatg caatcttgcc cagtgatttt ttttgtaaaa tcatgagtag aataaacaaa
     8101 ataaatgtca gttcgtcgat ataaatcaaa acaggatgag tatcagggac aaggcttgaa
     8161 gtaaatccga taatcaggta gcaaacagcc agcataccat gccaaataaa catgcctttg
     8221 tacatgtaat atatgtaaaa catcattgcg aatgctatag ggaaagacac tttccaggct
     8281 gtattttcac cccaaaaatc gtcggcaacc ataaaaataa gtgccggcag aaaataaaga
     8341 atgggattga aaaatgattc ccgaatttgt tgttttaata gtttcatgaa aaaatattgc
     8401 cgtaaaaaat cttcattttt ttaaaaaata tcctcagtct attcaataaa caaaatagac
     8461 taaggatagt tcactcgcat gaagtttaga gttggagcgg cttgcaggtt ttaatccgaa
     8521 acaaaaaaaa cagaaaatcc ggaataaaaa atttacgggt taaactccag catacgactg
     8581 atatacttgc cgataatatc aaattcgatg ttcacggttg tgcccacttt gatttggtga
     8641 aagttggtgt attcgtaagt gtagggaatt attgccaccc tgaacgaatt attggttggt
     8701 tccaccacgg tgaggcttac gccgtttaca gtcacagagc ctttatccac agtcatgtag
     8761 cctttgcggg cttttacagg atcaaattcg tactcaaaag taaaatacca gcttccgtcc
     8821 gcttcttcta cgtttttaca gatcgcagtc tgatccacgt g
//
LOCUS       DAORKP010000097         8823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1157_length_8823_cov_8.121807, whole genome shotgun sequence.
ACCESSION   DAORKP010000097 DAORKP010000000
VERSION     DAORKP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_8823_cov_8.121807"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLN75_06530"
     CDS             <1..410
                     /locus_tag="PLN75_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRG03806.1"
                     /translation="EDIDVLDLFAGTGSIGFEFISRGARSVVSVEQSDRHCVFIHKVC
                     NDLKIDNLMLIKGDVFRYIASARVKFDVIFADPPYELTKINEIPDLIFKQELLKPDGL
                     FVLEHSAKNNFEKHPNFSSHRHYGNVNYSFFEV"
     gene            complement(548..1453)
                     /locus_tag="PLN75_06535"
     CDS             complement(548..1453)
                     /locus_tag="PLN75_06535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRG03807.1"
                     /translation="MRRKSAYVPAPKLTRLTVKDETQLMDFLLAKMGGMSRSSVKSLL
                     AHRQISVNDKITTQFDFVLKPNDQVTVNTTRGNLELTHPKLRIIFEDTDLIVVEKKEG
                     LLTVTTGTNHDTTAFSILKNHVKKSSPANKIYTVHRLDRETSGVLVFTKNRDAQHTFR
                     NNWQEIVTKRTYVAVVEGKVERQKDRIVSWLTENEVSLKIKSSKTDNGGKEAITNYKL
                     LKAGEEFSLLEIELETGRKNQIRVHMESIGHPIAGDRKYGSTVNIGRLALHARVLEFY
                     HPVTGEIMHFETPVPKEFSKLVNNG"
     gene            complement(1475..2281)
                     /locus_tag="PLN75_06540"
     CDS             complement(1475..2281)
                     /locus_tag="PLN75_06540"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HRG03808.1"
                     /translation="MRFNTFIDSVKFFITRYKSALSGALLIIVSAIIISRISDELHPD
                     RIKDLYPEIVDCDRSKYATAPITARRLMNDKNDIQNIHAQANGLKDIYISNASFVEDS
                     ARLASEHILVRLKNNRLYHVKELTHSYPYATPEMADLLNTIGMHFYRKIKEKNLGYYR
                     FLVTSALRTNETQQDLTSRNSNASTQSAHLYGATVDITYKEFYNANTGNIEQNYLVAD
                     ALRETMLDLREQCLLLVVRERRQAVYHFTVVNCDPAKAPAEQITRKVLEY"
     gene            complement(2509..3015)
                     /locus_tag="PLN75_06545"
     CDS             complement(2509..3015)
                     /locus_tag="PLN75_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRG03809.1"
                     /translation="MKKIGLFYSHSTVKTAQIARKILQEFSDEEVEAVNLDEAWDDEF
                     NKFDNFILGTATWFDGELPDHWDEIIPKINTIKFRGKKVAIFGLGDQKNYPDNFVDGI
                     GLLAKVFEEIGAQIVGYTSTEGYTYGHSVAERDGQFCGLAIDFENQSRKSNERVKSWV
                     EQLRREFA"
     gene            complement(3087..5117)
                     /locus_tag="PLN75_06550"
     CDS             complement(3087..5117)
                     /locus_tag="PLN75_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRG03810.1"
                     /translation="MKKYDILTTDKETLRKWYSLLTLGRAIDDKAPVYLIQSLGWSYH
                     APYAGHDGIQLAIGQVFEKGVDYFFPYYRDMLSVLSAGMSVEEILMNGLSKAGDLSSG
                     GRHMSNHFAKLEWNIETVSSATASQNVHAVGVARAMVYYGQKGVAITSHGDSSASEGY
                     VYEAINGASNERLPVIFVIQDNGYGISVPKKDQTANRKAANNFSGFKNLKIIHCNGRD
                     VFDSMNAMTEAKQWAIENQSPVIVQANCVRIGSHSNSDKHTLYRDENELKYVKAADPL
                     YKFRRMLLRYNRFTEEELLEIEAESKKTLSAANKKVIAMPDPDPATILDHVLPEAYEP
                     KVYTDGTHHQDGEKTNLVTAINETLKAEFRHNPDTFLWGQDVANKDKGGVFNVTKGMQ
                     QEFGEKRIFNAPIAEEYIVGTANGMSRFDPKIKVVIEGAEFADYFWPAMNQYVECTHE
                     LWRSDGRFVPNVLLRLASGGYIGGGLYHSQNIEGALTTLPGARIVYPSFADDAAGLLR
                     TAMRTKGFTVFMEPKALYNATEAATFIPEDFEVPFGKARIRKQGEDLTLVTYGNTTHL
                     SLAAALQLSNEGYSVEVIDIRSLIPLDKDTIFESIKKTGKALIVHEDKVFSGFGAEIA
                     ATIGTEIFQYLDAPVQRVGSTFTPVGFHPKLEKAILPDTQRIYEAAKKLIEF"
     gene            complement(5136..6458)
                     /locus_tag="PLN75_06555"
     CDS             complement(5136..6458)
                     /locus_tag="PLN75_06555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRG03811.1"
                     /translation="MSKFEIKMPKLGESITEGTIVSLNIKTGDLVDEDQILFEVTTAK
                     VNAEVPSPVAGKVVEILFNEGDTVEVGKTVLLIDLEGASAEVAGSAAENQSPDTPAES
                     NQPENNSASASPAPKPEKPSNESERWYSPAVLQLAKQEAISVDELEKIQGTGYLGRLS
                     KKDLKNYIETGATRSTQTIQDKKLRPVEKPIETPLKSTPAYSVSMETSSGEVKDMDAV
                     GKIIADRMILSKQTSAHVTTVVEVDVTKLVNWRKKNKDEFRKQEGINLTYMPAIVQAT
                     VKALKEYPKLNASVDGYKYILKKNINIGVAVALEDENLVVPVIHNADKLNITGIAHQI
                     ENLSGKARSNKLAMSDIENGTFTITNFGTFKNIIGTPIINQPEVAILGVGYIEKKPAV
                     VETPEGDVIAIRHKMYLSLSYDHRLINGAVGGAFLLKIANYLENWSEK"
     gene            complement(6549..7268)
                     /locus_tag="PLN75_06560"
     CDS             complement(6549..7268)
                     /locus_tag="PLN75_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRG03812.1"
                     /translation="MQLYLENSSTDPYLNMAVEEFLLHHCNEPVFHVWQNEPSVIIGR
                     NQLADAEVNLDFIRKNDIKLVRRFSGGGAVYQDLGNINLTFINSNGNLDFDTYNLKVM
                     DFLTSLHLQPTSNQRRAIFINEKKVSGSSQHIRGQKVIYHATLLFSSNLDFLNRSLQG
                     KDMPEMPSKYVKSVKSPVTNLADHLPPDFTIEEFKQLIIRFFTGKDSRKIIFTEKETQ
                     EILVLKDFKYTNPDWINDGIL"
     gene            complement(7311..8660)
                     /gene="lpdA"
                     /locus_tag="PLN75_06565"
     CDS             complement(7311..8660)
                     /gene="lpdA"
                     /locus_tag="PLN75_06565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRG03813.1"
                     /translation="MNFDIAIIGGGPAGYNVAEKAALSGLKTVLFEKKAIGGVCLNEG
                     CIPTKTMLYSAKLLDNARAAAKYGILGTENAVFDLGKIITRKNKTVRKLTAGVRMKMK
                     ESGVTVVEHEAIIQGEKNEQIIISAGSETYSATYVLIATGSETFIPPIKGLSEVDFWT
                     SSEALDVKELPHTLAIIGGGVIGMEFASFFNSMGVKVTVIEMLPEILGAMDKELSAML
                     RSEYAKKGVDFHLNTKVVEVDSHSLTVEKNGTTSVIEAEKILVSTGRVANTKNLGLET
                     LSIELLRNGIKVNEYMQTSHPRVYACGDVTGSYMLAHTASRESEVALNHILGMDDKMS
                     YAANPNVVYTNPEISCVGKTEEELTENNIPFKVFKLPLAYSGRFIAENETGNGLCKII
                     TNENEQIIGCHILGNPSSEIISTACVAIEENYTLQKFQKIIFPHPTVAEIIHESLYL"
BASE COUNT         2184 a         1906 c         1922 g         2811 t
ORIGIN      
        1 ttgaagacat cgatgtgctt gatttgtttg caggcaccgg aagcatcggt tttgaattca
       61 tttcaagggg tgcacgaagt gtggtaagcg tggagcaaag cgaccgtcat tgtgttttta
      121 tccacaaagt gtgcaacgac ctgaaaattg ataatctgat gctaattaag ggcgatgtgt
      181 ttcgttacat cgcatctgcg agggtgaaat ttgatgtgat ttttgccgat ccgccctacg
      241 aactgaccaa aatcaatgaa attccggacc tgatttttaa gcaggaactg cttaagccgg
      301 atggattgtt tgtactggaa cattcggcca aaaacaactt tgaaaaacat ccgaatttct
      361 cgtcgcaccg ccattacggc aatgtgaatt atagtttttt tgaggtttag ctgataggtc
      421 agctcaccaa tcccctgaat gagaacttaa aaatgataat tgattagtta cctgaaacat
      481 ccaaacatcc aaacatccaa acatccaaac ttttcaaacg tccgaacatc cgaacttttc
      541 aaacatctca accgttgttc accagtttcg aaaactcttt cggaaccggt gtttcgaagt
      601 gcatgatttc accggtcacc ggatgataaa actccagcac acgtgcgtgc aaagccagtc
      661 gtccgatgtt aacggtggag ccgtatttcc tgtcgccggc tatcgggtgt ccgatgctct
      721 ccatgtgtac acgaatctga tttttccggc cggtttccaa ctctatttcc agtagcgaaa
      781 attcttcacc cgctttcagc agtttgtagt ttgtgatggc ttccttgccg ccgttatcag
      841 ttttactcga tttgattttc agcgaaactt cattttcggt aagccacgat acgattctgt
      901 ccttttgccg ctctactttt ccttccacca ctgctacgta ggtgcgtttg gttacaattt
      961 cctgccagtt attcctgaaa gtatgttgtg cgtcgcggtt tttcgtaaaa acaagtacac
     1021 cggacgtttc acgatccagc cggtgaacgg tgtaaatctt atttgccggt gaggatttct
     1081 ttacgtggtt tttcagaata gaaaatgctg tggtatcgtg attggtgccg gtagtcactg
     1141 taagcaatcc ctcttttttt tccaccacta tcagatcagt gtcttcaaag attatccgca
     1201 gtttcggatg ggtaagttcc agatttcctc ttgtggtatt cacagttacc tgatcgttag
     1261 gtttgagcac gaaatcaaat tgggtagtaa ttttatcgtt caccgaaatt tgccggtgtg
     1321 cgagcagcga tttgaccgag ctgcggctca tgcctcccat tttagccagc aggaagtcca
     1381 taagctgagt ttcatctttt acagtgagtc tggtgagttt tggagcaggt acgtaggcag
     1441 attttcggcg cataattgta aatttttatt tttgtcagta ttctaaaact tttcgtgtaa
     1501 tctgttcggc tggagctttc gcggggtcgc agtttaccac cgtgaaatga tatacagcct
     1561 gccttctttc acgtactacg agcaggaggc actgctcgcg caggtcgagc attgtttcgc
     1621 gcagtgcgtc ggctaccagg tagttttgtt caatgtttcc tgtattagca ttgtaaaatt
     1681 ccttgtaggt aatatccacc gttgcgccat acagatgagc cgattgagtg cttgcattgc
     1741 tgtttcggct tgtcagatcc tgttgggttt cgttggtgcg aagtgccgaa gtaaccaaaa
     1801 accggtaata tcccagattt ttctccttta tttttctgta aaaatgcatt ccaatggtat
     1861 taagcaggtc tgccatttcg ggagttgcgt aagggtagga gtgagtcagt tctttcacgt
     1921 ggtaaaggcg gttgttttta agccgtacca gaatatgttc gctggccagt cttgcgctgt
     1981 cttctacgaa cgaagcattg cttatgtaga tgtctttaag cccgttggcc tgagcgtgaa
     2041 tgttctgaat atcgttttta tcgttcataa gtcgcctggc tgtaataggc gctgtggcat
     2101 acttgctccg gtcgcaatcc acaatttcgg ggtataaatc ttttattctg tccggatgta
     2161 attcatctga aattcttgaa ataataatgg cagaaacgat aatgagcaat gcacctgaga
     2221 gtgcagattt gtaacgggta atgaaaaatt taactgaatc aatgaaagtg ttaaaacgca
     2281 tagtgaaaaa tttgcacaaa attacgcttt tacgacagca tttcaatgca tgttaacgaa
     2341 ttttgggaaa taaaatgcct tctgaaatgc atgtgataat gagtatgtgt gataaaattt
     2401 ctatcccaaa gaacaccttg ttcgtgtttt ttgggataga aacagattca gatttacagt
     2461 gaatgattga ttttctgaaa aaatggatta ccggacagaa ttcagtaatt acgcaaactc
     2521 tcttctcaat tgttccaccc agctttttac gcgttcgtta ctttttctgc tttgattctc
     2581 gaagtcaatg gcaagtccac agaactgacc gtctctttcg gcaaccgaat gcccgtaggt
     2641 atagccttct gttgaagtat aacccacaat ctgagctcca atttcttcaa aaacctttgc
     2701 cagcaatccg attccgtcca caaagttatc cgggtaattt ttctgatcgc ccagcccgaa
     2761 aatcgcaact tttttccccc tgaattttat ggtgttaatc tttggaatga tttcatccca
     2821 gtggtcgggg agttctccat caaaccaggt ggcagttccg aggataaagt tgtcgaactt
     2881 attgaattca tcgtcccagg cttcgtccag attcaccgct tcaacttctt catccgaaaa
     2941 ttcctgaaga atttttcggg ctatctgagc tgtttttacc gtgctgtggc tgtaaaataa
     3001 accaattttt ttcatttttt atatgcagtt atatttttga tcaatgttat ttttttttaa
     3061 tcagaatcca ccggtttgcc ggttttctaa aactcaatga gctttttggc tgcctcataa
     3121 attcgctgag tatccggtag aattgctttt tcaagtttcg gatgaaaccc gaccggcgta
     3181 aaggtagaac ctacccgctg aaccggtgca tccaggtact ggaaaatctc agttccgatg
     3241 gtagccgcaa tttctgctcc aaaaccggaa aataccttgt cctcgtgcac aatgagcgcc
     3301 tttccggttt tttttatcga ttcgaaaatg gtgtctttat ccagcggaat aagcgatcgg
     3361 atgtcaatta cctcaactga ataaccctca ttggataatt gcagggcagc tgccagcgac
     3421 aaatgtgtgg tattgccgta agtaaccaga gtcaggtctt caccctgttt gcgtattctg
     3481 gcttttccaa atggcacttc gaaatcttcc ggaataaaag tagcagcttc ggtggcatta
     3541 taaagcgctt ttggctccat aaatacagtg aaacctttcg tacgcatggc tgtgcgaagc
     3601 aagcctgcag catcatcggc aaaggatgga tacacgatcc tggcacccgg caatgtggtg
     3661 agtgcacctt caatattctg tgaatggtac aatccgcctc cgatataacc gccggaagcg
     3721 agtcggagca gcacattcgg aacaaaacgg ccgtcgcttc gccacaattc atgggtacat
     3781 tccacatact ggttcatggc aggccagaaa taatcggcaa actcggctcc ttcaattacc
     3841 actttaattt tcgggtcaaa acggctcatt ccgttggcgg tacctactat atactcttct
     3901 gctatcggag cattgaaaat gcgcttttcg ccaaattcct gctgcatccc ttttgttaca
     3961 ttgaatacgc cgcctttgtc tttgttggct acgtcctgtc cccagaggaa tgtatcggga
     4021 ttgtgtctga attcggcttt cagcgtttcg ttgatagccg ttaccaggtt tgttttttcg
     4081 ccatcctgat ggtgggttcc gtcggtgtaa actttcggtt cgtaagcctc aggaagcaca
     4141 tggtctaata tcgttgccgg atcgggatcg ggcatggcta ttaccttttt attggctgca
     4201 gatagcgttt tcttcgattc tgcctctatt tcaagcagtt cctcttcggt gaaacggttg
     4261 tagcggagca gcatccggcg gaatttgtac aacggatctg ctgcttttac atatttcaat
     4321 tcattttcat cacgatacaa ggtatgttta tccgaattgg agtgcgaccc gatgcgcaca
     4381 cagttggcct gcacaattac cggactttgg ttttcgatag cccattgttt tgcttctgtc
     4441 atggcgttca tcgaatcgaa tacatcccgg ccattgcaat ggattatttt cagattcttg
     4501 aatcctgaaa aattatttgc cgctttgcgg ttggcggtct ggtctttttt cggaacagaa
     4561 attccgtatc cattatcctg aataacaaaa atgaccggca accgctcgtt gctggcaccg
     4621 ttgatagcct catatacata tccttcggag gcacttgaat cgccgtgact ggtgatggca
     4681 actccttttt gtccgtaata aaccatggcg cgggctacac ccacagcatg tacgttttgc
     4741 gaagcagtag ccgaggacac agtttcaatg ttccattcca gtttggcaaa atggttggac
     4801 atgtgacggc cgccactcga taaatcgcct gctttagaaa gaccgttcat cagaatttct
     4861 tcaactgaca tgccggctga tagcaccgaa agcatatcgc ggtaataggg gaaaaagtaa
     4921 tcaacgcctt tttcaaatac ctgaccaata gccaactgaa tgccgtcatg acccgcataa
     4981 ggtgcatgat acgaccaacc gagcgactgt atgagatata cgggtgcttt atcgtcgatg
     5041 gcacggccaa gtgtaagcag cgaataccat ttccggagtg tttctttatc ggtggttaaa
     5101 atatcgtatt tcttcatttc tatttcgtta tttttttatt tttcagacca gttttcaaga
     5161 taattggcaa ttttcagcag gaatgcacca cctacggcgc cgttgataag ccggtggtcg
     5221 taggagagtg agaggtacat tttatgccgg atagcaatta catcaccttc gggcgtttcc
     5281 actacagcgg gtttcttttc gatgtatccc acgcccagaa tggctacttc aggctgattg
     5341 ataatgggag tgccgatgat gtttttgaat gttccgaaat tggtaatggt aaaagtgccg
     5401 ttttctatgt cggacatggc aagtttgttg cttcgtgctt tccccgacag attctcaatc
     5461 tgatgtgcta tgccagtgat attcagcttg tcggcattgt gaatgacggg cactaccaaa
     5521 ttttcatctt ccagtgctac agccactccg atgtttatat tttttttcag aatatattta
     5581 taaccatcta ctgaagcatt gagttttgga tattccttca gcgccttaac agtagcctgt
     5641 acaatggcag gcatataagt gaggtttatt ccttcctgtt ttctgaattc atccttgttt
     5701 ttcttccgcc agtttaccag ttttgttaca tccacttcca ctacggtggt aacgtgtgct
     5761 gaagtctgtt tggagagaat catccggtcg gctatgattt ttcctaccgc atccatatct
     5821 tttacttccc ccgacgaggt ttccatgctc acagagtatg cgggagttga tttgagagga
     5881 gtttcaatcg gcttctctac gggtcggagt tttttgtcct gaatggtttg ggtgcttctg
     5941 gttgcacctg tttcgatgta gttttttaag tcttttttac ttaaacgacc taaatagccg
     6001 gttccctgta tcttttccag ctcatcaaca gaaatggctt cctgctttgc cagttggagt
     6061 acagcgggtg aataccagcg ttctgattca tttgatggtt tttcgggttt cggagccggt
     6121 gatgcactgg ctgagttgtt ttccggttga ttactttcgg caggagtgtc cgggctctga
     6181 ttttcagcag ccgatccggc aacttcggca cttgcgcctt cgaggtcaat cagcaagacg
     6241 gttttgccta cttctaccgt atcgccttcg ttgaatagga tttctacaac tttgcctgcc
     6301 actggtgaag gcacttcggc atttactttg gcagtagtta cttcaaaaag aatctgatct
     6361 tcatccacca gatcgccggt ttttatattg aggctcacaa ttgtaccttc ggtgatactt
     6421 tcgccgagct tcggcatctt tatttcaaat ttcgacataa atagtttgat ggttagtgtt
     6481 tgttttttac cgtttcataa caaccaaact atttagaatg tttctaagtc tgtgtatttt
     6541 atgaaaaatt aaagtatccc gtcatttatc cagtccggat ttgtgtattt gaagtctttc
     6601 aaaaccagta tttcttgcgt ttctttttct gtaaaaatga ttttacgtga atcttttccg
     6661 gtaaaaaaac gaatgatgag ctgtttgaat tcttcgatgg tgaaatcggg cggaagatgg
     6721 tcggcaaggt ttgtgaccgg acttttcacc gattttacat acttcgatgg catttcgggc
     6781 atatctttgc cctgtaatga gcggtttaaa aaatcaagat tggaagaaaa cagcagggtg
     6841 gcatgataga taactttctg tccgcgaatg tgctgtgagc ttccggatac tttcttttcg
     6901 ttgatgaaaa tggctcgccg ttggtttgaa gttggctgca aatgcagcga ggtgagaaaa
     6961 tccatcactt tcaggttgta cgtatcaaaa tcaaggtttc cgttggaatt gataaaagtg
     7021 agattgatat tgcccaaatc ctgatataca gctccgcctc ccgaaaagcg tctgacaagt
     7081 ttaatgtcat ttttccggat aaaatcaaga tttacttcag catccgcaag ctgattgcgg
     7141 cctatgatga ccgaaggttc gttttgccag acatggaaaa ccggttcgtt gcaatgatgt
     7201 aacagaaatt cctccacagc catattcagg tatggatcgg tagaggaatt ttcaagatac
     7261 aattgcatgg ttgattggga tttacagatt aataatcaac tctcacacat ctacaggtac
     7321 aaactctcat gaataatctc agctactgta ggatgcggga aaatgatttt ctgaaacttc
     7381 tgcagtgtgt aattttcttc aatggcaaca caggcagtgg agatgatttc agatgatgga
     7441 ttcccgagga tgtgacaacc aatgatttgt tcattttcat tggtaatgat tttacacagg
     7501 ccgtttccgg tttcattttc agctatgaat ctgccggagt atgccagcgg cagtttgaaa
     7561 actttaaaag gtatattatt ttcggtaagt tcttcttcgg ttttaccaac acaggaaatc
     7621 tccggattgg tgtaaacaac attcggattg gcagcgtaac tcattttatc gtccatgcca
     7681 agaatatgat taagagccac ttcactttcg cgtgaggcag tatgagccag catgtaagag
     7741 ccggtcacgt caccgcaggc atacacccgc ggatggcttg tctgcatata ttcattcact
     7801 tttatgccgt tgcggagcaa ttcgatgctg agtgtttcca gtccgaggtt tttggtatta
     7861 gccactcttc cggtgctcac cagaattttt tcagcttcta tgactgaggt tgttccgttt
     7921 ttttccacag taagagagtg gctgtcaact tccactactt ttgtattcag gtgaaaatca
     7981 acaccttttt tggcatactc gctcctcagc atggccgaaa gctctttatc cattgctccg
     8041 agtatttcgg gcaacatttc aattactgtc acttttactc ccatgctgtt gaaaaaggag
     8101 gcaaattcca tcccgatcac tccgccgcca ataatggcaa gcgtgtgtgg cagttctttc
     8161 acatcgagtg cttcagacga agtccagaag tcaacttctg aaagaccttt gatgggcgga
     8221 atgaatgttt cggaaccggt agcaatgagt acatacgtag ctgaatacgt ttcacttccg
     8281 gcactaatga tgatttgttc atttttctct ccctgaatga tggcttcgtg ttccacaacg
     8341 gtgacaccac tttccttcat tttcatccgt acgccggcag taagtttcct cacggttttg
     8401 ttttttctgg ttatgatttt tccaagatca aaaactgcgt tttcggttcc cagaatgccg
     8461 tatttggcag cagcccgggc attgtcgagc aatttggcag agtaaagcat ggttttagtg
     8521 ggtatgcatc cttcgttcag gcatacaccg ccaatggctt ttttctcaaa taaaacagtt
     8581 ttcagcccac tcagagctgc tttttcggct acgttgtatc cggctggacc tccaccgatg
     8641 attgcaatat cgaaattcat acgattataa gttgttagaa ttaattagtt atcaattggt
     8701 tatttttcag aaatatccgg tttctaaaaa aagaaaatat aagtgataaa cattcaaaga
     8761 gggaattagt tttttaaaaa attaaaaaat ttcactttgc ataccgaaga tttatgataa
     8821 aat
//
LOCUS       DAORKP010000098         8780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1172_length_8780_cov_10.985444, whole genome shotgun sequence.
ACCESSION   DAORKP010000098 DAORKP010000000
VERSION     DAORKP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_8780_cov_10.985444"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1438
                     /locus_tag="PLN75_06570"
     CDS             173..1438
                     /locus_tag="PLN75_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRG03814.1"
                     /translation="MKSIIIAGIMLLATFAMPLQAAKPLKPWNKGAFETQKYRNVFVE
                     MGYKSEAVYAKLDEIFNSLFYGPNKIYFEVGDSMAYISDIKNHDVRTEGMSYGLMIAV
                     QLDKKEIFDRLWRWGKKYMQHQEGPMKGYFAWSLKTDGTRNSQGPASDGELYYVTALI
                     FASNRWGNDTGINYLAEAQNILNCSEEKNGEKGVLPLIDREHQLITFVPVGFGARFTD
                     PSYHLPAFYEVWARWAEDGRADYWRECAVKSREYLHKSIHPVTGLNPDYNNYDGTLLN
                     SGRIIGDAFRFDSWRVPMNIALDYSWSNVDKKWQQNYANTIQKFLYSKGLTTFVDQFN
                     VDGTDVKEILPAGEHKALRHSLGLVATSAAVSLAAKNKMSREFVDQFWNAKHEPYEDG
                     YFDAYYDGLLRLFAFMHLSGNYQIIFPKK"
     gene            1576..3534
                     /locus_tag="PLN75_06575"
     CDS             1576..3534
                     /locus_tag="PLN75_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRG03815.1"
                     /translation="MKKIQNKLFVFVLFCILCAGCSEDIPNPENPSPPKPDEETPVVV
                     SKNKLPKLHVDGRFLKNESGENITLHGFAQTYSPFFNQNAWNNYSVSGCLTYNKRMID
                     RIMGTGWKMNFVRMHMDPYWSDDPTQASVRYEGHERFSQERFKKYLDEVFVPMAEHAI
                     SKGLYVVLRPPGVSPGKIAPGDDYNKFLELVWDIVSKHPKIKNNSDIMFELANEPINI
                     LGPNGTYASSGQGHFDQMKLFCQSIVDKIRANADNIIWVPGLAYQSSFSGFANNPVKG
                     TNIGYAVHAYPGWYGSDTEEASPELGGSMGGGYESFQRGWNAQVKPVADIAPIMVTEM
                     DWAPKKYEASWGKSFTGVVGGSGFGANFKYIADITGNVSWLIFTECHRLAEFNDIAGT
                     AGNYTFLNDPEACVWPTFHWYKEYASGKEDSVTESELAKIEVVGVSGNLTMLTGGDRY
                     LIVNAIYNNGTSLPVTSKTVFTTSDQSVVSINENGRLIALKDGSATITATFTPSTGVS
                     KQVSFTVYSTTFPLTKELFNPSIFAQGTFDEETRTLVTGQWGFGGWWYNKGVDLSKYK
                     YLIAELGNDNNSSVSFRLFDENNYWSQPATYDFGNSRKVVVELGNMYKKINGVQVKLN
                     PAQIYIIGFWSSGGSPIIIKGVYLSDSL"
     gene            3750..>5759
                     /locus_tag="PLN75_06580"
     CDS             3750..>5759
                     /locus_tag="PLN75_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03816.1"
                     /translation="MSQTKTAPRKNLSERFVHKSIGNPYLPLWEHLPDGEPRVFEDPD
                     NPGKYRAYIIGSHDLRVNSYCGPDIRVWSAPVEDLSSWRDEGPVFTYFIDNQWDVMYA
                     PDLVEVKRKDGTKEYYLYPHSRGPQREAMVAKGSRPDGPFKPINLTEDGRRTLPGSIL
                     GFDPAIYVDYITDPNDPDYEIGFRAYAYWGFQRSLAAELDQKTMYSLRPGTEIIPYFL
                     PSSARYGVIRDPEGTKYPQLYANEDPKNFNFFEASSIRKVGNKYITVFSGYSGPEYGL
                     GSTNSSLRYAVADSPLGPWKSGGVLVDSRGPVLNQDGSGLQTTSGGHNTHGSIENING
                     QWYVFYHKPPRNFGFARQAVVAPITIEWDEKPVAEGGSAVIRAYDPYAKNKIWTAKDN
                     QGREYTGAEITSEGFHIYGLDPYQYYSAGYASYLSDVSIQQDSWDIWDNHMPITNVKN
                     GHIIGYKYFGFGGLKKATKGLNAFKGTKRGNKTEFNLFLTPKTSKSFKVNVWLDGPWD
                     NDTWKGKKIGEIIVPANSKQNVTKFTVNVSKSVDKLKKKHAIFLVAEGDGSEELFDLS
                     GLGFSSKKNKIERPIVPTVSISVNGQKIDLPKTPVRSTNENGIVGYDIYEATYKLPAE
                     TTIVPKVTATVDNNDVKIKVTQADSATGTAKVEFDYNGVVKTYRVV"
     assembly_gap    5760..5859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5890..7251
                     /locus_tag="PLN75_06585"
     CDS             5890..7251
                     /locus_tag="PLN75_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG03817.1"
                     /translation="MMKNKVISAVVFLMVLVLSNAQNPIIQTMYTADPAPMVYNGKLY
                     LYTSHDEDNSSWFVMNNWKLYSTTDMVNWTDHGVVLSYSDFSWAKGDAWAAQCIERDG
                     KFYMYVPVTSRLNNRPAIGVAVADSPYGPFYDPLGKPLVQSGHGDIDPTVFIDDDGQA
                     YLYWGNPNCYYVELNEDMISFKNDIVRVPMTEESFGKREGNVKDRPTLYEEAPWLYKR
                     KDMYYLLWGGGPIPEHLGYSTGKSPKGPWKNQGMLMPTEGRSFTNHPGIIDYKGKTYL
                     FYHNGALPGGHGFNRSVCVEEVKFNKDGTIQPMKMTEGIQSGLETLNPYRKNEAETIA
                     FSEGVKASQNKQVGVFVNAMQNNAYTKVKGVDFGTQGASRFSARVGTTINGGVTMEIR
                     LGSKDEKLIGTIRVPITGGDDRWEVVSTDIEKVTGTQDLYFVFKSDKPGRIMYFDYWM
                     FSK"
     gene            7376..>8780
                     /locus_tag="PLN75_06590"
     CDS             7376..>8780
                     /locus_tag="PLN75_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HRG03818.1"
                     /translation="MSIVRVGDTYYMSSTTMHMSPGVPIMKSTDLVNWKLVNYAYDTL
                     DDVDALNLNNGKNAYGGGSWASCIRYVNNTFYVSTFAGTTGTTYIYSTKDIEKGPWEV
                     KKFKPSYHDHTIFFDDDGKIYMIWGAGRLRMIELKEDLTGVKEGTERVLIENASAPAG
                     NNIMLPAEGSQLFKVNGKYYLFNITWPRNSMRTVVIHRADKITGPYEGRLALQDLGVA
                     QGGLIDTPDGNWFAYLFRDNGAVGRIPYLVPVKWEDGWPVLGENGKVPVSLNLPASKG
                     LIPGIVASDDFKRKKNEPKLPLVWQWNHNPDNSKWDVNGKKGYFRLTTGRIDSDILSA
                     KNMLTQRTIGPTCVGETKINVFKMKEGDVAGLMLLQARYGYAGVKFENGKKYIVMVNA
                     GGDKPEEVERIPLKGKKVYFKATCDFTNMRDMADFYYSLDGKNWNKIGTPLRMRYTLP
                     HFMGYRYGLFNYATKTTG"
BASE COUNT         2702 a         1627 c         2016 g         2335 t
ORIGIN      
        1 attgtttcag tgagcatgaa atattaatat agaaaatcat gatacatcca ttagcgtaat
       61 tacgatttta gacgtacaaa ggattgatta ttaataagat aagctttaca atctattatt
      121 aaagctcaat aattccccct tcagggggtt agggggcaga tataagaaaa ttatgaaatc
      181 aattattata gccggcatta tgttattagc aacatttgca atgcctcttc aggcggcaaa
      241 accactaaaa ccttggaata aaggagcttt tgagactcag aaataccgaa atgtattcgt
      301 agaaatgggg tacaaatcgg aagcggtgta tgctaaactg gatgagattt tcaactctct
      361 tttctacgga ccaaacaaga tttattttga agttggggat tcgatggcat acatttctga
      421 catcaaaaat cacgatgttc gcacggaagg aatgtcctac ggactgatga ttgccgttca
      481 gttagataaa aaagaaatat ttgaccggct gtggcgctgg ggaaaaaagt acatgcagca
      541 tcaggaaggg ccgatgaaag ggtatttcgc gtggagtttg aaaaccgacg gcacgcgcaa
      601 ttcacaagga cctgcttctg acggagaact ttactatgtc acggcactaa tctttgcttc
      661 gaatcgctgg ggaaacgata ccggtataaa ttacttagcc gaagcgcaga atatcctcaa
      721 ttgctcagag gaaaagaatg gtgaaaaagg agttcttcct ttgatagacc gtgagcatca
      781 gctgattaca tttgttcctg taggttttgg agccagattt accgatcctt cctaccacct
      841 acctgcattt tatgaagttt gggcaagatg ggctgaagat ggtcgtgccg attactggcg
      901 tgaatgtgcc gtgaaaagtc gtgaatactt gcataaatcc attcatccgg tgaccggatt
      961 gaatcctgat tacaacaatt atgacggaac tttgctcaat agcggacgga ttatcggtga
     1021 tgcattccga tttgattcct ggcgcgtccc gatgaatatt gcattggatt actcatggtc
     1081 aaacgtagat aaaaaatggc agcaaaacta cgccaatacc attcagaaat tcttgtattc
     1141 caaaggattg accacttttg tagatcaatt taatgtggat ggtacggacg taaaagaaat
     1201 tcttcctgcc ggcgaacaca aagctttgag gcattcgctt ggcttggtag caacatctgc
     1261 tgcagtttca ttggctgcaa agaataaaat gagccgagaa ttcgtcgatc agttttggaa
     1321 cgcaaaacac gaaccgtatg aagacggcta tttcgatgct tattacgatg gattgttgag
     1381 gttatttgca tttatgcatt tgagtgggaa ttatcagatt atttttccca aaaaataggt
     1441 aatagaaacc aaatccgttc ctgcgaaata actgatgatg ttgttttcat gatgttataa
     1501 actatttaat tgcttaataa gaattacagt tgttagcgtt gattttgacc gttttacatc
     1561 agctttaatt aaaacatgaa aaagattcaa aataaactgt ttgtttttgt gttattctgt
     1621 attttgtgtg caggatgttc agaagatata cctaacccgg agaatccttc acctccaaaa
     1681 ccagatgaag aaacacctgt tgtggtatct aaaaataaac ttccaaaatt acatgttgac
     1741 ggtcgtttcc ttaaaaatga gtctggtgaa aatattaccc tgcatggatt tgcgcaaaca
     1801 tacagtccgt ttttcaacca aaatgcatgg aataattaca gcgtatccgg gtgtttaaca
     1861 tataacaaac ggatgattga ccgcattatg ggcaccggat ggaagatgaa ttttgtgaga
     1921 atgcacatgg atccatactg gagcgacgac ccaactcagg catctgttcg ttatgagggg
     1981 catgaacgtt tttctcagga gcggttcaag aaatacctgg acgaagtgtt tgttccaatg
     2041 gctgaacatg ccatcagcaa agggttatat gtggtgttgc gtcccccggg cgtaagtccg
     2101 ggaaaaattg cgccgggaga tgattacaat aaatttcttg agttagtatg ggacattgtg
     2161 tcaaaacatc ctaaaatcaa aaataattcc gatataatgt ttgaactggc taatgagcct
     2221 ataaatatat tgggaccaaa tggtacgtat gcaagtagcg gacaggggca ctttgatcag
     2281 atgaaattat tctgtcaaag cattgttgat aagattaggg caaatgccga taatataatt
     2341 tgggtaccgg ggctggccta ccagtcttct ttcagtggat ttgctaataa tccggttaaa
     2401 ggaacaaata ttggttatgc tgttcacgca tatccgggat ggtacggtag cgataccgaa
     2461 gaggcaagtc cggagttggg aggttcgatg ggaggaggat atgagagttt tcagcgtggg
     2521 tggaatgcac aggtgaaacc cgtagcagat attgctccta tcatggtcac tgaaatggat
     2581 tgggcaccca aaaaatatga agcatcatgg ggcaaaagtt ttaccggagt ggtaggaggt
     2641 tctggttttg gagctaattt taaatatatc gcagacataa cagggaatgt gagttggttg
     2701 atttttaccg aatgtcatcg ccttgctgag tttaatgata ttgcaggaac agctggaaat
     2761 tacactttcc tgaatgatcc tgaagcttgt gtgtggccaa cattccactg gtataaagaa
     2821 tatgcatcag gtaaagagga ttcggtaacg gaaagtgaat tagcaaagat agaagttgta
     2881 ggcgtgagcg gaaacttgac aatgttaacc ggaggagaca ggtatttgat tgtaaatgca
     2941 atttacaaca acggcacatc tttgccggtt acatcaaaaa cagtgttcac aacttcagac
     3001 caatctgttg taagtataaa tgagaatgga aggttgatag cattaaaaga tggatcagca
     3061 accataacgg ccacatttac accttcaaca ggagttagta aacaggttag ctttactgtt
     3121 tactcaacta cctttcctct gactaaagag ttgtttaatc catctatttt tgcgcaaggg
     3181 acgttcgacg aggaaacgcg aaccttagtt accggacaat ggggctttgg aggatggtgg
     3241 tataacaaag gagtagattt gtcaaaatat aaatacctca ttgctgaact tggtaacgat
     3301 aataatagca gtgtctcttt cagacttttt gatgaaaaca attattggtc tcagccggcc
     3361 acgtatgatt ttggcaactc aagaaaggta gtcgtggaac ttgggaacat gtataaaaaa
     3421 ataaatggag tacaggtcaa acttaatccg gcacaaatct atattattgg cttttggtca
     3481 tcgggtggca gtccgataat cataaaaggt gtttatctgt cggatagtct ttaataaaaa
     3541 tccgggttgg tgcttacacc attattttaa ctatggtaga agcaatgaaa tttgctcaaa
     3601 aagcaatggt tattttcgct tgttgttaat gatatatgtg ttgaattttt attaataatg
     3661 tattttatat gaagagatta gaatttttac agaaagagag atacgcgatt tttttcatcg
     3721 cgatgttctt attttcctcg accggattga tgagccagac taaaacagct ccaagaaaaa
     3781 atctatcaga aagatttgtc cataaatcaa taggaaatcc ctatttaccg ctttgggagc
     3841 acctgcctga cggcgaaccg cgcgtatttg aagatcctga taatccggga aaataccgtg
     3901 cttacatcat tggttcgcac gatttgcgtg taaatagcta ttgcggaccc gatatccgcg
     3961 tttggtcggc accggtagaa gatctgtcaa gttggcgcga tgaaggtccc gtttttacat
     4021 attttattga taaccaatgg gatgtgatgt acgcacctga tttggtagag gtgaaaagaa
     4081 aagacggcac caaagaatat tacctttatc cacacagccg cggtcctcaa agagaagcaa
     4141 tggtagccaa aggaagccgt cctgatggtc cgtttaagcc cattaatctt accgaagatg
     4201 gaagaagaac gcttcccggc agtattctcg gttttgaccc ggccatttac gttgattaca
     4261 tcacggatcc aaacgatcct gattatgaaa ttggtttcag agcatacgcc tattggggtt
     4321 ttcaacgctc attagcagcc gaattagacc aaaaaacaat gtattcgctt cgtcccggca
     4381 ctgagattat tccatatttc ttgccatcca gcgccagata cggtgttatc cgagaccctg
     4441 aaggaacaaa atatcctcaa ttatacgcga atgaagatcc gaaaaatttc aatttctttg
     4501 aagcatcatc catccggaaa gttggaaaca aatacatcac ggttttcagc ggttattccg
     4561 gacctgaata tggattagga agtactaatt cttcgttgcg ttatgcggtt gccgattcac
     4621 cgctcgggcc ctggaaaagc ggtggcgttt tggttgactc gcggggacct gttttgaatc
     4681 aggacggctc gggattgcag accaccagcg gtggccacaa cacgcacggc agcatcgaaa
     4741 atattaacgg ccaatggtac gtgttttatc ataaaccacc aaggaatttc ggttttgcac
     4801 gtcaggcagt ggtagctccg attacgatag aatgggatga aaaaccagta gcagaaggcg
     4861 gtagcgcagt aatcagagca tatgatcctt atgcaaaaaa taaaatttgg acagcaaaag
     4921 acaaccaggg aagagaatac acgggtgctg aaattacgtc ggaaggcttt catatctacg
     4981 ggcttgatcc gtaccaatat tattctgcag gctatgcaag ctacctttcc gatgtcagca
     5041 ttcagcaaga ttcatgggat atttgggata atcacatgcc gataacaaat gtgaaaaacg
     5101 gccatattat cgggtataaa tattttggat ttggaggact taaaaaagct accaaaggtc
     5161 ttaatgcttt caaaggaacc aaaagaggga ataaaacaga attcaacttg tttctcactc
     5221 ctaaaacttc aaaaagtttc aaagtaaatg tttggttaga cggtccttgg gacaacgata
     5281 cttggaaggg gaaaaagata ggtgaaatta ttgtccctgc taattcgaaa cagaacgtaa
     5341 caaaatttac tgtaaatgtt tctaaatcag tagataaatt gaaaaagaaa cacgcgatat
     5401 ttttagttgc agaaggcgat ggatcggaag aactgttcga tttaagcgga cttggtttca
     5461 gctcaaagaa aaataaaatt gagcgtccta ttgtaccgac tgtttctatt tctgtgaacg
     5521 gacaaaagat tgatttgccg aaaacacccg ttagatctac caatgaaaac ggaattgttg
     5581 ggtacgatat ttacgaagca acctataaac tccctgctga aacgacaatc gttcctaaag
     5641 tgacggcgac agttgacaat aacgatgtga aaataaaagt aactcaggca gattctgcaa
     5701 caggaacagc aaaagtagaa tttgattaca acggagttgt aaagacctat agggttgttn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttttcacaaa caattaaaaa
     5881 agctgcaaaa tgatgaagaa taaagtaata tcagccgttg tgtttttgat ggttttggtg
     5941 ctttcgaatg ctcagaaccc aattattcaa accatgtaca ctgccgatcc ggcaccgatg
     6001 gtgtacaatg gcaaactgta tctctacacc agccacgacg aggacaattc ctcctggttt
     6061 gtgatgaata actggaaact ttattctact accgacatgg taaattggac tgatcacggc
     6121 gtggtacttt catactctga tttttcatgg gcaaagggtg atgcttgggc ggcacaatgc
     6181 attgagagag acggaaaatt ttacatgtac gtgccagtta catcccgatt aaataaccgc
     6241 cctgcgattg gagtggctgt tgcagatagt ccttacggac ctttctacga tccgctcgga
     6301 aaaccgctgg tgcaaagcgg tcacggtgat attgacccca ccgtattcat tgacgatgac
     6361 ggacaagcgt acctctactg gggaaatccg aactgctact acgtggagtt gaacgaagat
     6421 atgatttctt ttaaaaatga tattgtacgg gttccaatga ccgaagaatc ttttggaaaa
     6481 agggaaggaa atgtgaaaga ccgaccgact ttgtacgagg aagcaccttg gctctacaag
     6541 cggaaagaca tgtactacct gctttggggt ggcggaccca ttcccgaaca tttgggttat
     6601 tcaaccggca aaagtccgaa aggaccctgg aaaaatcagg gaatgctgat gcctactgaa
     6661 ggcagaagtt tcaccaatca tccaggaatc attgattaca aagggaaaac ctaccttttt
     6721 taccacaacg gagcacttcc cggcgggcac ggtttcaacc gctcggtgtg cgtggaagaa
     6781 gtcaaattca acaaagatgg aaccatacag ccgatgaaaa tgaccgaagg aatccaaagc
     6841 ggactggaaa ctttgaatcc atacagaaaa aacgaagcag aaaccatcgc tttttccgaa
     6901 ggagtaaaag cttctcagaa taagcaagtg ggcgtatttg tgaatgcgat gcagaataac
     6961 gcatacacaa aagttaaagg cgttgatttt ggaactcagg gagcatcccg attttcagct
     7021 cgcgtaggca caaccatcaa cggaggtgtg acaatggaaa tccgcctcgg aagcaaggat
     7081 gaaaagctta ttggaacgat aagagtaccg attacaggcg gtgacgaccg ttgggaagtg
     7141 gtgagtaccg atattgagaa agttaccgga acgcaagacc tgtattttgt tttcaaatcc
     7201 gataaaccgg gaagaatcat gtatttcgat tactggatgt tttcaaaata gaaaggaata
     7261 accgatgaaa atattactta aaaaacttat agttgcaact ttcctcataa ttggaattgc
     7321 atcagcgtcg gctcagaaag ccgaaaatcc aatcgtattt gctgacgtgc cggacatgtc
     7381 catcgtaagg gttggcgaca cctattacat gagcagtacc accatgcaca tgagtccggg
     7441 tgtgcccatt atgaaatcga ccgatttggt gaactggaaa ctcgtaaact atgcttacga
     7501 cacgttagat gatgttgatg cactaaatct gaataatgga aaaaacgcct acggaggtgg
     7561 ttcctgggca agctgcattc gctacgtcaa caatacgttc tatgtttcca cttttgcggg
     7621 tactaccgga acgacctaca tttattccac caaagacatt gaaaaaggac cctgggaggt
     7681 gaaaaaattt aagccttcct accacgacca taccattttc tttgatgacg acggaaaaat
     7741 ttacatgatt tggggagccg gacgattgcg aatgattgaa ttgaaagagg atcttacggg
     7801 agtgaaagaa ggaacggaaa gggttttgat tgaaaatgcc agcgctcctg cgggaaacaa
     7861 tattatgttg cctgctgagg gttcacagct tttcaaagtg aatgggaaat actacctttt
     7921 caatatcacc tggccacgta acagcatgcg tacggtcgtt attcaccggg ctgacaaaat
     7981 caccggccct tatgaaggac gccttgcttt gcaggatttg ggagttgcac agggaggatt
     8041 gattgatacg ccagacggta attggtttgc atacttgttt cgtgacaacg gtgctgtggg
     8101 acgaattccg tacttagttc ctgtgaaatg ggaagacggt tggcctgtat tgggagaaaa
     8161 tggaaaagta ccggtttcgc tgaatctgcc tgcaagcaaa gggttgattc cgggaattgt
     8221 tgcttcggat gatttcaaaa gaaagaaaaa tgagcccaag cttccacttg tatggcagtg
     8281 gaaccacaat ccggataatt ctaaatggga tgtcaacggg aaaaaaggtt atttccgtct
     8341 gacaaccgga cgcattgata gcgacattct ttcggcgaaa aatatgctta ctcagcgtac
     8401 catcggccca acctgcgtag gcgaaaccaa aatcaacgtt ttcaaaatga aagaaggaga
     8461 tgtagctggc ttgatgttgc ttcaggcaag gtacggttac gctggtgtca aatttgaaaa
     8521 tggaaaaaaa tacattgtga tggtcaatgc cggcggtgat aaacctgaag aggtggaacg
     8581 gattccattg aaaggcaaaa aagtttattt caaagccacg tgtgatttca ccaatatgcg
     8641 tgatatggcc gatttttact acagccttga tggaaaaaat tggaacaaaa tcggaactcc
     8701 gttgcgtatg agatacacgc ttccgcactt tatgggctat cgttacggtt tgttcaatta
     8761 tgccactaaa accacaggcg
//
LOCUS       DAORKP010000099         8732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1189_length_8732_cov_8.437478, whole genome shotgun sequence.
ACCESSION   DAORKP010000099 DAORKP010000000
VERSION     DAORKP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_8732_cov_8.437478"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..787)
                     /locus_tag="PLN75_06595"
     CDS             complement(131..787)
                     /locus_tag="PLN75_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRG03819.1"
                     /translation="MIEILDNLDKQWLLALNNDYSVFWDGIMFAFSEKITWIPLYISM
                     IYVIYRRWKKESWWLILALILSVVFADQIASGILKDWVARLRPSHDPSLAGQVVLVNG
                     YKSGLYGFVSSHAANSFALALLSSLFFRNRIFTIFILLWAVINAYSRIYLGVHYPGDV
                     LGGALVGVFVAVVLFLLLKKIRPPLFAEYRTGEIKYMIPVWIFLITCVGLIGYSVWFF
                     "
     gene            complement(981..1652)
                     /locus_tag="PLN75_06600"
     CDS             complement(981..1652)
                     /locus_tag="PLN75_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03820.1"
                     /translation="MANQLYKQGQYADAARLYENELKKGVAAELYYNLGNSYYKSNEI
                     GMAILNYERALRLDPDYEDARYNLKIAESKIVDNVNSTPTFFVKRLFNALITSNTSNH
                     WTVISFVCFIVALVLFFFFVFSRNRRNRKYGFFGMFLFAGLFVTALIFSGIRKDQFLN
                     RNEAIVMSGAVTAKSSPDKSGTDIFQLHEGTKVLVKSTLSGWTEITLENGAVGWVEES
                     AIARI"
     gene            complement(1750..3573)
                     /locus_tag="PLN75_06605"
     CDS             complement(1750..3573)
                     /locus_tag="PLN75_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRG03821.1"
                     /translation="MKRVLFILSVLAWMAGWNVNAQDEVEFTASAPDVAYIDTPFQLI
                     YSVNSSAKDLQAPDFQFFEILAGPFESRSSYTQIVNGKRSASTSLSYTFTLMPSKAGT
                     FKIPGASIVVDGKKIHSNDLTIRVENAEKNATQQTQGGAATGGTTQRVTGESIFVRTI
                     VSKTNIYEQEAISVTYKLYTLLDVAQFTGAKLPDFNGFLKQDIEQSQNKQLSAETYKG
                     RSYGTVVLYQAVLFPQHTGEIEIGKASFTALIRLQNKAQVRSIFDDFFDSYTNVERTL
                     TAPAVTVRVNELPLGGKPATFSGAVGNFNLTSTLSSGNLKTNEAATIKVVISGNGNMK
                     LLKNPEIKFPDGFEVYDPKVDNSFSTGSGGVSGTKTIEYMFIPRRSGKYDIPSAELSY
                     FDLNNRTYRTLRTPPYKINVTKGEGGETVVENFTGKEEVTQIAKDIRYIYTGKIEIKP
                     ESRPWFGTMPFWTVFLIPLILAGILFVYLRKMAKENANLDFVKTKKANKVAQKRLNLA
                     HKFLSEGKKNQFYDEVMKAVWTYLSDKLSIPVAELNKENIRFKMEAKGIETDLIEDFT
                     NILHTCEYASYAPGSGQLEMGNFYQETVEAISKVEEHFKKK"
     gene            complement(3651..4337)
                     /locus_tag="PLN75_06610"
     CDS             complement(3651..4337)
                     /locus_tag="PLN75_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03822.1"
                     /translation="MKFFQYISFLLLLFPLSVIAQKESDDIRAGNRLYKEKKYTEAEI
                     EYRKALQKNNKSFEANYNLGNALFRQGKYDKAFEHYKNALPLTSDKQKTAYDFHNLGN
                     TFMSGGKIEEAISAYKTALKNNPKDNETRYNLAYAMSQLKKQQQDQNQNQNDNGNDKD
                     QNQNQDNEKQQPKNNPDKKEEQPQPQQPQMSKENAQQILEALQQDELDTQEKAKNAQK
                     RTVRKTDKDW"
     gene            complement(4403..5428)
                     /locus_tag="PLN75_06615"
     CDS             complement(4403..5428)
                     /locus_tag="PLN75_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRG03823.1"
                     /translation="MFRFAHPEYLLLIVPVALLVGFFIRSQILRIKNTKLWGNPDILD
                     PLMPNVSYVRPQIKFYLQLLVLILLVLVLAQPQFGTKEENVKKKGIEVMIALDVSNSM
                     LAQDIQPSRLQKAQQILSQLIDRMKDDKVGMIVFAGNAYVQLPITTDFMAAKMFLSTI
                     NTSMVPVQGTSIGSAIDLAIKSFGEKKTDAGRAIIVLTDGENHEDDAVAAAKLAADNG
                     IKVYVVGMGTPEGGPIPIGGTVSFKKDRDGNVVVSALNEQMCSGIAQAGGGLYVRADN
                     SDAALKSISKQLDTLATADLEEKVFSQYNEQFQSFAIIALILLLIEFFIFSRKNKWLS
                     RIRIFDV"
     gene            complement(5607..6590)
                     /locus_tag="PLN75_06620"
     CDS             complement(5607..6590)
                     /locus_tag="PLN75_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRG03824.1"
                     /translation="MSFKNPEYLFLLLLLLPIVFWYIWELRKSDASMQVSAISKLKGY
                     PKTLRMKFRHLPFILRTLAIGLLIVAIARPQSSSNWRTENKEGIDIMMALDISGTMQA
                     EDLRPSRLEAAKNVAIDFIQSRPNDNIGLVVFAAESFTQCPLTSDHSVLINLFNGIRY
                     GLINDGTAIGLGLANAVSRIKDSKAKSKVIILLTDGSNNQGDIAPITAAEIAKTFGIR
                     VYAIGVGSHGTINMPVQTPFGVRYQQVESEFDEKTLKDIAEMTGGQYFRATNNAKLRA
                     IYQEIDQLEKTKISVKEFSNKSELYFLFALLAFLLLFAENLLRNTWMRTLP"
     gene            complement(6706..7680)
                     /locus_tag="PLN75_06625"
     CDS             complement(6706..7680)
                     /locus_tag="PLN75_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HRG03825.1"
                     /translation="MNKLFRFCFILFLFLCTEYTSAQPVTVKARMDSTQIWIGDQTAL
                     TFEVVQNKGQKVKMPLFSDTIVGNLEIVEQAKSDTFNIADDKVQVNVKYIVTSFEDSL
                     IYVPPFPYISGSDTSWSNSLSLKVIQPFVIDSTSNQLADIKPVFKPKFNWKEFAKQMM
                     LVFLLIVLAVLLYFLVRKYVMRKPILKTETRKVIIPPHVEALQRLDKIREEKLWQKGR
                     MKEYHTELTETLRIYIERMFNINSMEMTSEEILTHTSFMKVDKSGAYGALRQILTLAD
                     LVKFAKWNPSPNDNEMSLMNAYLFVNQTKVEEFRPSEKITDEIKLNNE"
     gene            complement(7974..>8732)
                     /locus_tag="PLN75_06630"
     CDS             complement(7974..>8732)
                     /locus_tag="PLN75_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRG03826.1"
                     /translation="GMTFSEVREYQYGDDVRSIDWNVTARFGRPYIKVFEEERELTVM
                     LLVDVSGSLDFGTTHQFKKGIITEVSATLAFSTIQNNDKVGAIFFSDKIEKFIPPKKG
                     KKHVLHIIRELLVFEPASNQTNIGEALKYFTNAIKKRSTAFIISDFIDSGFERTLTIA
                     NNKHDIAAMQVYDIREAELPDVGLIKVKDAETGERIWVDTSDKRLRTSYKHKWNERQL
                     ALQKTFKQSGVDSVTMSTSEDYVRALMKLFKLRG"
BASE COUNT         2354 a         1858 c         1803 g         2717 t
ORIGIN      
        1 caggaagcgc attgaattcg atgaagaaat aaaagcaaat tgaatcatta tgaaaattaa
       61 aatcccaaaa gtttacgata acttttggga tttattattt tttaattctc ggtgaataag
      121 tgagttttct ttaaaaaaac caaacgctgt atccgatcaa cccgacacag gtaataagaa
      181 aaatccaaac aggaatcatg tatttaattt caccggtgcg gtactccgca aacaaaggcg
      241 gacggatttt ttttagtaac aggaataaaa caactgccac aaaaacccca accaaagcac
      301 ctcccagtac atcgcccgga taatgcacac ccaggtaaat gcgcgaataa gcgttgataa
      361 cagcccacag aagaataaaa atagtgaaaa tcctgtttcg gaaaaataag gaagaaagca
      421 gtgccaaagc aaaagaattg gcagcatgag aggatacaaa accgtaaagc ccacttttat
      481 agccgttgac aagcactacc tgacctgcca gagacggatc atggctcggt ctgagacgcg
      541 ccacccagtc tttgagaatg cctgaagcaa tctgatcggc aaaaaccaca ctcagtatca
      601 acgccaggat tagccaccac gactcctttt tccaacgcct gtagatgacg taaatcatag
      661 aaatgtacag cggtatccag gtgatttttt cgctgaaagc gaacatgatt ccgtcccaaa
      721 aaacggaata atcgttattc aaagccagca gccattgttt gtccaggtta tcgagaattt
      781 ctatcataag gcgttttttg taagttaaat gcttgcctgg tttatccctg cctgcggcag
      841 gcaggccgat atgtcggggc ctatggccgg ttagaaattt aaaattagaa attagaaatt
      901 agaaagcctg ccttcaattt agaacacaga acacagaaca cagaacacag aatacagaac
      961 acagaactcg aaactcaaat ctatattctt gcaatagcgc tttcttccac ccatcctacg
     1021 gctccgtttt cgagggtaat ctcagtccat cccgacaagg tgcttttaac caacactttg
     1081 gttccttcgt gcaactgaaa gatgtctgtg ccgcttttgt cgggcgaact tttggctgta
     1141 acggctccac tcatcacgat ggcttcgttg cgattcagaa actgatcttt cctgattccc
     1201 gagaaaataa gtgctgtgac aaacaatccg gcaaaaagaa acatgccaaa aaatccgtac
     1261 ttacggtttc ttctgttgcg cgaaaacaca aaaaagaaaa atagcaccaa cgcgacaata
     1321 aaacagacaa aactgatgac agtccagtga ttggaagtgt ttgaagttat aagcgcatta
     1381 aagagacgtt ttacaaagaa agtgggagtg gaatttacgt tgtcaacaat tttactttct
     1441 gcaattttca ggttgtagcg ggcatcttcg taatccggat cgagccggag cgctctttcg
     1501 taattcagga ttgccatccc tatttcgttg gatttgtaat aactattgcc cagattatag
     1561 taaagttcag cagctactcc ctttttcagt tcattttcat aaagtctggc agcatcagcg
     1621 tactgaccct gtttgtacag ttgatttgcc atctgtatgt ttttgttttc agatgaaata
     1681 ctgttttgtg cctgtaatgt aacagtgatg atcaaaacaa aacagaaaaa tacatagata
     1741 cgtttcattt tattttttct taaaatgttc ttccactttg cttatggctt ctaccgtttc
     1801 ctggtagaaa ttgcccatct ccagctgacc tgacccgggt gcgtagcttg catactcaca
     1861 cgtatggagg atgttggtaa aatcctcaat caaatctgtt tcaatacctt tcgcttccat
     1921 tttgaaacga atattttcct tgttcagttc cgcaaccgga atcgacaact tatcggacaa
     1981 gtaagtccat acggctttca tcacttcatc atagaattga tttttctttc cttccgagag
     2041 gaatttgtga gcaagattga gtcgcttctg agccacttta tttgcttttt tggttttcac
     2101 aaaatcaaga tttgcatttt cttttgccat ttttcttaaa taaacaaaca aaatgccagc
     2161 cagtatgagc gggataagaa ataccgtcca aaaaggcatt gtaccgaacc agggtcggga
     2221 ttcgggtttt atttcgattt tgcctgtata gatgtatcgg atatcttttg ctatctgagt
     2281 gacttcttcc ttccccgtga agttttccac cacggtttct cctccttcgc ctttggtcac
     2341 attgattttg taaggcggtg tacgcaatgt acgataggtt ctgttattca aatcgaaata
     2401 ggataattcg gccgaaggaa tatcatattt cccagatcga cggggaatga acatgtattc
     2461 gatggttttg gtacccgaaa ctcctcccga accggtgctg aaactgttgt ccactttcgg
     2521 atcataaact tcaaatccat ccgggaattt tatttcagga tttttcagca gtttcatgtt
     2581 accgttgccg gaaatgacca ctttaatggt ggcagcttcg ttggttttca gattgccgga
     2641 gctcagtgta gaagtgagat taaaattgcc aacggctccc gaaaatgtgg caggtttacc
     2701 tccgaggggc aattcgttga cccgtactgt tacagccggt gcggtgagag ttctttccac
     2761 gtttgtatag gagtcgaaga aatcgtcgaa gatgctgcgt acctgcgctt tgttctggag
     2821 tcggatgagt gccgtgaaac tggcttttcc gatttcaatt tcacccgtat gctgtggaaa
     2881 taaaacagcc tggtaaagca ctacagtgcc ataactccgg cctttgtaag tttcggcaga
     2941 aagctgtttg ttttgcgatt gctcgatgtc ctgtttcaga aatccgttaa aatcagggag
     3001 tttagctccg gtaaactgag ccacatccag cagcgtataa agtttatagg taacggaaat
     3061 agcttcctgc tcgtagatat ttgtttttga aacaatggtg cggacaaaaa tactttcgcc
     3121 cgtcacacgc tgcgtagtgc cacctgtagc agcgccgccc tgagtttgtt gcgtggcgtt
     3181 tttttcagcg ttttctaccc ggatggtcag atcattggaa tggatctttt tcccgtccac
     3241 tacgatgctt gctcccggaa ttttgaatgt tccggccttt gagggcatca gggtgaatgt
     3301 ataggaaaga ctggtagatg cagatcgttt gccatttaca atttgagtgt atgaactcct
     3361 ggactcaaaa ggtcctgcga gtatttcaaa aaactgaaaa tcaggtgcct gaagatcttt
     3421 ggcggaagaa tttacagagt aaatgagctg aaagggagtg tcgatatagg caacatcagg
     3481 agcagatgca gtaaactcca cttcatcctg agcatttacg ttccatccgg ccatccatgc
     3541 caagaccgat agtatgaata atactctttt catttttttt gaattcttcg tttatttttc
     3601 ttcgctgtaa tccgcggatg ccaatatttc ctggctggtt ttcagaaaaa ttaccagtct
     3661 ttatccgtct ttctgaccgt acgtttctgt gcgtttttag ctttttcctg tgtatccagt
     3721 tcgtcttgct gcaatgcttc cagtatctgt tgagcgtttt ctttcgacat ttgaggctgt
     3781 tgaggctgag gctgttcctc ctttttatcc ggattattct taggttgttg tttttcgtta
     3841 tcctgatttt gattctgatc tttatcgttg ccgttgtcgt tttgattttg attttgatct
     3901 tgctgctgtt ttttcagttg cgacattgcg taagccaggt tgtaacgggt ttcattgtct
     3961 ttcggattat ttttcaacgc cgttttataa gccgaaatag cttcctctat ttttccaccc
     4021 gacatgaatg tattgccaag attgtgaaaa tcatacgctg ttttttgttt gtcggaagta
     4081 agtggtaatg catttttgta gtgctcgaat gctttatcgt atttaccctg tctgaaaagt
     4141 gcatttccga gattatagtt cgcttcaaat gatttgttgt ttttttgcag tgcctttcga
     4201 tattcaattt cggcttcggt atattttttt tccttgtata gcctgtttcc ggcacgtata
     4261 tcgtcactct ccttttgagc tatcactgac aaaggaaata acaacagcaa aaaagatatg
     4321 tattggaaaa acttcatagt ttagttctga gttctaagtt ttgagttttg agttttgagt
     4381 tttgagtttt gagttctgag ttctaaacat caaatattct tatccggctc agccatttgt
     4441 tttttcggct gaaaataaag aattcaataa gcaaaagtat gagtgcaatg atagcaaacg
     4501 actggaattg ttcgttgtac tgcgagaata ccttttcctc gaggtcggct gtagccagcg
     4561 tatcaagttg tttgctgatg cttttcagag cagcatcaga attatctgcc cgcacgtaaa
     4621 gtccgccgcc tgcctgagca atacctgagc acatctgctc gttgagtgcg gataccacca
     4681 cattgccatc gcggtctttc ttgaagctta cggttcctcc aatgggaatc ggaccacctt
     4741 cgggcgtgcc catacccact acatatactt ttattccgtt gtcggcagcc agttttgcag
     4801 ctgctaccgc gtcgtcttcg tgattttcgc catcggtgag tacaattatt gctcttccgg
     4861 catctgtttt cttttctccg aatgatttga tagccaaatc gatggccgaa ccgatggaag
     4921 ttccctgcac cggaaccatg ctggtgttga tagtggaaag aaacattttt gcggccatga
     4981 agtcagtcgt aatgggaagc tgaacatacg catttccggc aaatacaatc atcccgacct
     5041 tatcatcctt catccggtcg ataagctgag agagaatctg ctgggctttc tgaaggcggc
     5101 tgggctgaat atcctgcgcc agcatagaat tggagacatc gagtgcaatc attacttcta
     5161 tgcccttctt tttcacattc tcctctttgg taccaaactg tggttgcgcc agtaccagca
     5221 ccagcaatat caacaccagc agctgcaggt aaaattttat ctgcggacgt acgtaggata
     5281 cattaggcat gagcggatcc agtatatccg gatttcccca gagtttggta ttcttaattc
     5341 tcaaaatctg ggaacgaata aaaaacccta ccaacaatgc taccggtacg attaataaca
     5401 gatattcagg atgtgcaaat ctaaacattt ttatattaga ataatcagaa gaaaacaaat
     5461 ttgacccaaa ccactgatgg gagaaattaa ttagcgtttg ttaatatttt ttcaacagta
     5521 agtattataa attacacctt ttctttttta agaaaacttt tatctccatc ccaattttcc
     5581 cccttcggga gtcagggggc taaaatctac ggcaatgttc tcatccaggt attgcgaagc
     5641 agattttcgg caaacaatag taaaaaagcc agcagagcaa acaagaaata caattcgctt
     5701 ttattgctga attccttcac gcttatcttt gtcttttcga gctggtcaat ttcctgatag
     5761 atagctctta atttagcatt attagttgcc ctgaagtact gtccgccagt catttcggca
     5821 atatccttca gggttttttc gtcaaattcg gactccacct gctggtatct gactccaaaa
     5881 ggagtttgta cgggcatatt gattgtgccg tgggaaccaa ctccgatggc atacacccga
     5941 ataccgaatg ttttggctat ttcggctgca gtaatgggtg caatatctcc ctgattatta
     6001 gagccgtcgg taagcagtat aatcactttt gattttgctt tactgtcctt aatacgactc
     6061 acagcattgg ccaggccaag tccgatggca gtcccatcat tgatcagtcc gtatcttata
     6121 ccattgaaga gattaatcag cacagaatga tcgctggtga gcggacattg cgtaaaactt
     6181 tcggcagcaa aaaccaccag accgatgttg tcattcggac gcgactgtat aaaatcgatt
     6241 gccacgtttt ttgcagcttc gagtctgctc ggccggaggt cttcggcctg catggttccg
     6301 gatatatcca gcgccatcat gatgtctatt ccctccttgt tctcggtacg ccagttgctt
     6361 gacgactgcg gacgggcaat ggccacaata agcaaaccga tagccagcgt tctgaggatg
     6421 aatggcaagt gcctgaattt catccgcaac gttttgggat atcctttcag cttgctgata
     6481 gcagaaacct gcatgctggc atcggacttt cgcagctccc agatatacca aaatacaata
     6541 ggcagcaaaa gcaggagtaa aaacagatat tcaggattct taaatgacat ttttttatta
     6601 aaatacttcg ttcaacagtt gtttgtttca tacgggtgca ctgtcagttt tcagtcagca
     6661 gcacttgttt tccaatactc tatactcaat tgttcggaaa tttatttact cattatttaa
     6721 ttttatttca tcggttattt tttccgatgg tctgaattcc tccactttgg tttgatttac
     6781 aaacagatac gcattcatca ggctcatttc attgtcgttg ggagagggat tccatttagc
     6841 gaatttcacc aggtcagcca gcgtaagtat ctgccttaat gctccgtaag cgcccgattt
     6901 atcgactttc atgaaactgg tatgagtcag aatttcttca gaagtcattt ccatgctgtt
     6961 aatattgaac attctttcga tataaatacg cagtgtttca gtaagttcgg tatgatattc
     7021 cttcattcgg cctttttgcc agagtttttc ttctcttatt ttatccagtc gctggagtgc
     7081 ttccacgtgt ggcgggataa tcactttgcg ggtttcggtt ttcagaatgg gcttgcgcat
     7141 tacatatttt ctaaccagga aataaagcag caccgccagc acaataagca gaaatacaag
     7201 catcatctgt ttggcaaatt ccttccagtt gaatttcggc ttgaaaacgg gcttgatatc
     7261 agccagttgg ttggaggtcg agtcgattac aaacggttga attaccttga gtgaaagaga
     7321 attggaccac gaggtatcgc tcccggagat gtacggaaac ggcggaacat aaatcaatga
     7381 atcttcgaat gaagtgacaa tgtattttac gttcacctgt actttgtcgt cggcaatgtt
     7441 gaatgtatcg gactttgcct gctccacaat ctcgagattg cctactatcg tatccgaaaa
     7501 aagaggcatc ttcactttct gtcctttatt ctgaacgact tcaaaagtaa gtgcagtctg
     7561 atcgcctatc cagatttgag tggaatccat ccttgctttc accgtaacag gctgtgcaga
     7621 agtatattct gtacaaagaa acaggaagag aataaagcaa aaacgaaata acttgttcat
     7681 tttatttagt aatgtattcg tttcttttat agaagtcatc ttgacttgca aaacatagag
     7741 aacaatagtc ttattttgtc agtttttata tttattttaa tgttcttcga aaacaatagt
     7801 ttaaaaatat cgaagatatt ttttctatgt ttccgaaaat atgagaataa gtgtaaatta
     7861 ttctatgttt tctattgttt aaaaaagtcg agacaagttt ataaaaaaag tctgatcccg
     7921 atagtaatca gagagttctg gtgtaaataa taaactataa actgctaact atttcaccct
     7981 ctcaacttga aaagtttcat taaagcgcgt acataatctt cgctcgtgct catggttacc
     8041 gaatccaccc ccgattgctt gaaagtcttt tgcagtgcga gctggcgttc gttccacttg
     8101 tgtttataag atgtacggag acgtttatca gaagtatcta cccagatacg ttcgccggtt
     8161 tcggcgtctt tcaccttgat gaggcctacg tcgggcagtt ctgcctcacg aatatcatac
     8221 acctgcatgg ctgctatgtc gtgcttgttg ttggcaatgg tgagcgtacg ctcgaatccc
     8281 gaatcgatga aatcagaaat gataaatgcc gtcgaacgtt ttttgatggc gttggtaaaa
     8341 tatttcaatg cctctccgat gttggtctga ttgctcgcag gttcgaaaac cagtaactca
     8401 cgaatgatgt gcagcacgtg ttttttccct tttttcggcg gaataaattt ttcgatttta
     8461 tccgaaaaga aaatggctcc tactttatcg ttattctgta tggttgaaaa tgccagtgta
     8521 gccgaaactt cggtgatgat gccttttttg aactgatgcg tagttccgaa atcgagactt
     8581 cccgaaacat cgaccagcag catcacggtg agttcacgct cttcttcgaa caccttgata
     8641 tacggacgac caaaacgggc agtcacgttc cagtcgatgg accggacatc atcgccatat
     8701 tgatattcac gcacttcgga gaaggtcata cc
//
LOCUS       DAORKP010000100         8547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1234_length_8547_cov_10.034856, whole genome shotgun sequence.
ACCESSION   DAORKP010000100 DAORKP010000000
VERSION     DAORKP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_8547_cov_10.034856"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1318)
                     /gene="pdxA"
                     /locus_tag="PLN75_06635"
     CDS             complement(242..1318)
                     /gene="pdxA"
                     /locus_tag="PLN75_06635"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRG03827.1"
                     /translation="MEPDKIIIGITHGDINGIGYEVILKALADNRTLDMFTPIIYGSS
                     KLAGFYKKNLDIQGINFNIIHHAGEASSKRINIINCVDDEVKIDVGQSTSEAGTAAFA
                     ALEKATEDLKNKKIDALVTAPINKNNIQSESFHFPGHTEYLEEKFGEKSSSLMMLVSD
                     AIRIAVVTGHIPVSQVSGTITEELILKKLTVFNHSLKKDFSIVRPRIAVLGLNPHAGD
                     NGVIGDEEQKIIVPALKKAEQQGIMCFGPYAADGFFGSGNFKNFDGVLAMYHDQGLIP
                     FKTMAMDNGVNFTAGLAVIRTSPDHGTAYDLAGQNKASELSFLQALYLATDVFRNRSI
                     YKEMHVNPLPVSEPKHDSRKNSIE"
     gene            complement(1337..4387)
                     /locus_tag="PLN75_06640"
     CDS             complement(1337..4387)
                     /locus_tag="PLN75_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRG03828.1"
                     /translation="MKNFFKNKHIYLLLIFSLISVFSYSQDQLIRILEDSLTVLGKKY
                     ASVGYVKIKKITSNDDQRRITLTTNETLSYLPLRPENVDSIYNLSKRIIGLTYPDYSV
                     SIITDNRELEDLIPGYFRKNRKSPYNRFKVSPENFPLVKNLSAPFTVTNGLQNRHLAV
                     WQSHGKYYNQSRQEWVWQRPVVFQTVEDLYTQAYVLPFLVPMLENAGANVMLPRERDT
                     QINEIIVDADRSTGNSTFKADEKIWQKSTQAGFGNTQLTYTEKQNPFRLGYYMHAKTV
                     KDSLKSSKATWIPDIPESGRYAVYVSYKSVPNSTRDARYTVKHKGGETHFSVNQTMGG
                     GTWIYLGHFDFEKGKSSEMCVTLSTYSSFKDKDVTADAVKIGGGTGNIARKRADEVIK
                     TTVGKGRKKRTVTSVKKYSNYSVSRLPRFTEGARYWLQWAGVPDSVYSRTEGKNDYSD
                     DFQSRGYWVNYLAGGSSVLPQQEGLKIPLDMAFAFHSDAGINKNDSVIGTLAIFTVEN
                     TDKKKIYKNNISRWNAREMTDIIQTQIVDDIRKSFYSDWNRRGLWNRNYSESRVPEVP
                     TMLLELLSHQNFEDMRLGHDPRFRFLVSRAVYKGMLRYLATVNDFEYVVQPLPVDEFS
                     TRFISDSTIELSWKARPDKLEPTAIPDSYIVYTRTEDGGFDNGVMVKKNVFTKKIEKG
                     KIYSFRVEAVNRGGKSFPSEILSVFKAPNSNPSVLIINAFNRISGPESFTEGTELAGF
                     NNDFDAGVPYISDISFTGRQTEFRRSKPYVNDQNVGHGASNDDFGHQVIAGNTFDYPY
                     IHGKAIKAAGHSFVSASEASVVSGKVKPADYKIVDLILGKERQQIDPLDSTSTDFKTF
                     VPEMRKAITELTDAGGNLMISGSNIVSDLVIADKSTNFERRFMENTLKIKWKSDKIGN
                     VGQVVFKPVNNKHFQHVQQLSFYSIPNSVSYHVEAPDVILPAGKKAVSLCDYKENGYS
                     AGIAYKEKYGICAFGFPFETIKSADDQNMLMKSVLQFFTDKNIH"
     gene            complement(4633..6747)
                     /locus_tag="PLN75_06645"
     CDS             complement(4633..6747)
                     /locus_tag="PLN75_06645"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRG03829.1"
                     /translation="MATLQTIRNRGVLLAIIIGLALLAFVIGDFLNSGSTLFHQAKRN
                     VADVAGEKIDIETYQKAIDQMNTVYKIEYGRNDFNENEMSQMRTQVWESLINEKIMAM
                     EAKKMGLTVSKEELTDRLIGNNIHPMIMQRRAFADPQTGQFSKNSLLQFYNSIFSDEV
                     PQQDQEQLQEAKSYWLFWENAVKNTILQEKYISLIGKAVGANTIEAKYNFDARKMTGD
                     VNYIVQNYSTISDSTISVSDSELKALYEKQKELFKQEDNRSISYVSFEVTPLQEDFKE
                     AEAWIAKVSEEFKTTDDVAGLVNTESDISYTGENYSRQTVPANLKEFAFSNGNGAVFG
                     PLFQNNTYTMARIMESGIMESDSVKLRMIVLQPNEEKKADSIVNAIKSGASFTEMVAK
                     HSLPQAAANGGEVGWITKGMVGKEIADPAFSKSTNETFKVSGMQGIQIFQIMEKTPAR
                     PKVKLAILERKVTPSNQSYGKIFNEAKQFAGASTDSKKFEEIAKQKGYVIRPAIGLMK
                     TTDQVDAIPQSRQVVRWAFENKPGVISDVFDCDRNTYLVATVTEVNPKGYRTLKQVTP
                     QLKAQIIKDKKADVLKKQLSDMLAKNPTLEVLATTLGVEVKTAPSVNFASFQFGDAGP
                     EPYIIGKASVTADNKITAPLKGETGVFVIQPLSKIPDATAFNAKIEAAQLDGRTSQSL
                     PYAIMQKLKEKYNVVDNRFNYY"
     gene            complement(6893..8143)
                     /locus_tag="PLN75_06650"
     CDS             complement(6893..8143)
                     /locus_tag="PLN75_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRG03830.1"
                     /translation="MNLIGYIILSLAGILFFAGIETAYMASNRLRFELAKKDKSLNSY
                     IISIFYNNPQQFISTLFVGKYISLVAYGVLIAMWLVEPLASMGLAFSLILQIIIAATI
                     LVIVGEFLPKTVFRLHPNLWLRIFSWLLLLFYFLLYPFSRIISWLSLKLFRFSKTEIP
                     ASTEENVFSRIDLNYLLQENLEANEDIKDLNNDVKIFQNVLDFSKVKLRDCYVPRTEM
                     VAHEYGVNVNTLKQTFIETGLSKILIYKEDIDNIVGYIHSSEMFKHSKDWVKHIKPIP
                     IVPENMAAQKLMKIFMQQKKSIAVVVDEFGGTAGIVTLEDIMEEIFGEIEDEHDFKDI
                     TAEKTAENEYLFAGRLEVEEANQMFGLSIPVSEDYSTIAGYILHAHQHFPKVNEIVRV
                     DRFTFKCLKVTNNRIELVKMQVNK"
BASE COUNT         2273 a         1721 c         1719 g         2834 t
ORIGIN      
        1 actacaaacg gtgaggtatc ccacatcatg cttttaaccg atgaacaaag attgctggca
       61 aaattgtttg attttgggtg acccagtgtc gaagtttcat tttgccataa taataagcaa
      121 ttaatgcagt atttagcaat ataacattac tatatctaac ttcgaagccg acttttgctt
      181 tcaaaaagaa agcaaaaatt cattttgaga cagcctcttt tctgtttttt tcagttacga
      241 attattcgat actgtttttt ctcgaatcat gtttgggttc gctgacagga agcggattta
      301 catgcatttc tttgtagata gaacggtttc ggaatacgtc cgtagcgaga taaagtgcct
      361 gaagaaaaga taattcagaa gccttgttct gtccggcaag gtcgtaggct gtgccatgat
      421 caggtgaagt gcggataacc gcaagtccgg cggtgaaatt aacgccgttg tccatcgcca
      481 tggttttgaa agggataagt ccctggtcgt gatacatagc cagcaccccg tcaaaattct
      541 tgaaattacc cgaaccgaaa aatccatcgg cagcataagg gccaaaacac atgattccct
      601 gctgttcggc ttttttcaaa gccggaacga tgattttttg ttcttcatca cctatcacgc
      661 cgttatcccc ggcatgggga ttcagaccta acacagcaat tcttggtcgc acgatggaga
      721 agtctttctt cagcgaatga ttaaaaacgg taagtttctt cagaatcaat tcctccgtaa
      781 tggtacctga aacctgactt accggaatgt gtcccgtaac aacagcgata cgaatagcat
      841 cgctcacaag catcatgagg gatgaagatt tttcgccgaa tttttcctcc aggtattctg
      901 tatgaccggg aaaatgaaag gattcggatt gaatgttgtt tttgttgatg ggtgcagtta
      961 ccagagcatc tattttcttg tttttcaggt cttcagtagc tttttcgagt gcagcaaatg
     1021 cagctgttcc ggcttccgaa gtggactggc cgacatcaat tttcacttca tcgtccacac
     1081 aattaatgat gttgatgcgc ttgctgctgg cttctcctgc atgatggatg atgttgaaat
     1141 taatcccctg aatgtccagg ttttttttgt aaaatcctgc cagtttagac gaaccgtata
     1201 tgattggggt aaacatatca agcgtccggt tgtctgccag tgctttaaga ataacttcgt
     1261 agcctatccc gttgatatcg ccgtgtgtta ttccgattat gattttgtca ggttccatag
     1321 ttaattttat attgaattaa tgaatatttt tatccgtaaa aaattggaga acacttttca
     1381 tcagcatatt ctgatcatca gcagatttga tagtttcaaa cggaaatccg aaagcacata
     1441 tcccgtattt ttctttatag gcaatgcccg cgctgtagcc attctccttg taatcgcata
     1501 agctaacggc ttttttgccg gccggaagta ttacgtcagg tgcttctaca tggtaggata
     1561 cactattcgg aatggaataa aatgaaagtt gttgcacgtg ttgaaaatgt ttgttgttta
     1621 caggtttgaa tactacttgc cctacgttcc cgatcttgtc cgatttccat tttattttca
     1681 gtgtattctc cataaatcgc cgctcaaagt tagtactctt atcagctatt accaaatccg
     1741 agacgatgtt tgaacctgaa atcatcaaat ttccacccgc atccgtcaat tccgtgatgg
     1801 cttttcgcat ttcaggtaca aatgttttga aatctgtact ggttgagtcg agcggatcaa
     1861 tctgctgtcg ttccttaccc agtataagat ctactatttt gtaatctgcc ggttttactt
     1921 ttccgcttac taccgatgcc tcacttgcag aaacgaatga gtgaccggct gctttgatag
     1981 ctttgccatg tatataggga tagtcgaacg tattacctgc tattacctga tgaccaaaat
     2041 catcattact tgctccatgc cccacattct ggtcgtttac gtaaggtttc gaacgcctga
     2101 attccgtctg tcgacccgta aagctgatat ctgaaatgta gggcaccccg gcatcaaagt
     2161 cgttgttgaa gccggctaac tcagttcctt cagtaaaact ttccggtcct gaaattcggt
     2221 taaatgcatt gattatcaat acagaagggt tggagttggg ggctttaaat accgatagta
     2281 tttcggatgg gaaacttttt cctcccctgt ttactgcttc caccctgaaa ctgtaaattt
     2341 tacctttttc tatttttttt gtaaatacat ttttcttcac catcactccg ttgtcaaatc
     2401 ctccgtcttc tgttcgggta taaacaatgt atgaatccgg aatggcagta ggctccagtt
     2461 tgtcaggtct tgctttccaa ctgagttcaa tggtggaatc ggatatgaat cgggtgctga
     2521 attcatctac aggtaacggc tgaaccacat attcaaaatc atttacagta gccagatagc
     2581 gcagcatccc tttataaaca gctctgctta ccaaaaatct gaatcgcggg tcatgtccga
     2641 gacgcatatc ttcaaaattc tgatgtgaca acagctcaag tagcatggtg ggaacttcgg
     2701 gtactcttga ttcgctgtaa tttcggttcc agagtccccg ccggttccag tcggagtaaa
     2761 atgattttcg gatatcatcc actatctgag tttgaatgat atcagtcatt tctctggcgt
     2821 tccatcggga gatgttattt ttgtaaattt tctttttatc ggtattttcc actgtaaaaa
     2881 tggccagtgt gcctattaca gagtcattct tattgattcc ggcatccgaa tgaaaagcga
     2941 aagccatatc gagtggaatt ttcagacctt cctgctgagg caaaacggaa gaaccgccgg
     3001 caagataatt tacccaatat cctcgcgact gaaaatcgtc cgagtaatca ttttttcctt
     3061 cggtacggct gtagacacta tcgggtacac cggcccattg cagccagtag cgtgcacctt
     3121 cggtgaaacg aggtaaccgg ctcacagagt agttagaata ctttttgacg gaagtaaccg
     3181 tacgtttctt tcgtcctttc cctacggtgg tttttatcac ttcatccgct cttttccggg
     3241 ctatgttgcc cgtgcctccg ccaattttta cggcgtctgc cgtcacatct ttatccttaa
     3301 agctgctgta agtagaaaga gttacacaca tttcgctgct ttttcctttc tcaaagtcaa
     3361 aatgtccgag atatatccac gttccgcctc ccatggtttg attcactgag aaatgtgttt
     3421 ccccgccttt gtgctttacg gtgtagcggg catcccgggt actgtttggc accgatttgt
     3481 aagatacata tacagcatat ctgccacttt caggtatatc aggaatccag gtggctttcg
     3541 aagatttcag tgaatctttt actgtttttg cgtgcatgta atatccaagt ctgaatggat
     3601 tttgtttttc ggtgtatgta agctgagtat tgccgaatcc ggcctgagtg cttttttgcc
     3661 atattttttc gtcagccttg aaagtggaat ttccagtgct tctatcggca tctactatta
     3721 tttcattgat ttgtgtgtcg cgttcgcgtg gaagcattac gttcgctccc gcgttttcga
     3781 gcattggcac aagaaacggg agtacgtaag cctgtgtata aagatcttca acggtctgaa
     3841 acactaccgg tctttgccac acccattcct gtcgtgactg gttgtagtat ttgccatgac
     3901 tttgccagac agccagatgc cggttctgga gtccattggt aacggtaaaa ggtgcggata
     3961 agtttttcac cagtggaaaa ttctccggcg ataccttgaa acggttatac ggacttttcc
     4021 tgtttttcct gaaatatccg ggtatcaaat cttccagttc tctgttgtcg gtaattatgc
     4081 ttaccgaata atcaggataa gtcaggccga taattctttt tgacaggttg taaatcgaat
     4141 caacattttc aggtctgagt ggcaaataac tcagtgtttc gttggtagta agtgttatac
     4201 gtcgctgatc atcatttgaa gtgatttttt taatttttac ataacccaca ctggcatact
     4261 ttttacccaa tacagtaagc gaatcttcca atatcctgat caactgatcc tgtgaatagg
     4321 aaaatactga aattaaactg aatatgagca gcagatatat gtgtttgttc ttgaaaaagt
     4381 ttttcatcag agtatttttt ttaaaaagaa tcatcaggag tcaatacgat gacctacgcc
     4441 ggcttacagt gaaagggcct ggttgaaaag tattaaactt tagtaattca gagccggata
     4501 ttttgttttg aaagtaagat atagttttga tattcggcaa acaatacaaa aatacggttt
     4561 ttagcttaaa atacaaaaac ggagcaaagc gagtttactc cgtttttaca tgtgatgtat
     4621 tttttctttt aattaatagt aattgaagcg attatcaact acgttgtatt tttctttcag
     4681 tttctgcatg attgcataag gaagtgattg agaggtacgt ccatcgagtt gtgctgcttc
     4741 aatttttgca ttaaatgcag tagcatcagg aattttggaa agcggttgaa tcacaaacac
     4801 tccggtttca cctttcagcg gtgctgttat tttgttgtcg gcggtaacag aagcttttcc
     4861 gatgatataa ggctcgggtc ctgcatcacc aaactggaat gaagcaaaat taactgatgg
     4921 tgcggttttt acctcaacac ccagggtggt agccagtact tccagtgtag gatttttagc
     4981 cagcatgtcg ctcaattgtt ttttcagcac atcggctttc ttgtctttaa taatttgtgc
     5041 tttcaattgt ggagtaacct gtttgagggt tctgtatcct tttggattga cttctgtaac
     5101 ggttgctacc aggtatgtgt ttctgtcgca gtcaaaaaca tctgaaatta cacccggttt
     5161 gttttcaaaa gcccaccgca ctacctggcg cgactgtgga attgcatcta cctggtcggt
     5221 ggttttcatc agaccaatgg caggacgtat tacatatcct ttctgtttgg caatttcttc
     5281 gaatttttta ctgtcggtgc ttgctccggc aaactgtttt gcttcgttga agatttttcc
     5341 gtatgactgg ttgcttggtg tcactttcct ttcaaggatt gctaatttta ccttgggtct
     5401 tgcaggtgtt ttttccataa tttggaaaat ctgtatgcct tgcattccgc ttactttgaa
     5461 agtttcattg gttgatttcg aaaatgcggg atcagctatt tcttttccca ccatgccttt
     5521 ggttatccat cctacttcac ctccgttggc tgcagcttgt ggcaaggagt gcttagctac
     5581 catttcagta aagctggcac cgctcttaat ggcgtttaca atgctgtcag ctttcttttc
     5641 ttcattgggt tgcagtacta tcatgcggag ttttacagag tcggactcca taattcccga
     5701 ctccataatg cgagccattg tataggtatt gttttgaaaa agaggaccaa atacggcacc
     5761 gtttccatta ctgaaagcaa attctttcag gttagccggc actgtctgac gtgaatagtt
     5821 ttcacctgta tagcttatgt ccgactctgt attgaccagg cctgctacat catcggttgt
     5881 tttgaattct tcgcttactt ttgctatcca tgcttcagct tctttgaagt cttcctgaag
     5941 gggagtcacc tcaaacgaaa cataactgat tgaacggtta tcttcctgtt tgaatagttc
     6001 tttttgtttt tcgtaaagtg ctttcagttc tgagtcagat acgctgattg ttgaatccga
     6061 aatggttgaa taattttgaa cgatataatt tacatctccg gtcattttgc gggcatcgaa
     6121 attgtatttt gcttcaatag tattggctcc cacggctttt ccgattaatg atatgtattt
     6181 ttcctgtaag atagtgttct ttacagcatt ctcccaaaac aaccaatatg atttggcttc
     6241 ctgaagttgc tcttggtctt gttgaggtac ttcgtcggag aaaatactgt tatagaactg
     6301 gagtaagctg tttttgctga actgaccggt ttgcggatct gcaaatgcac gtcgctgcat
     6361 gatcatcgga tggatgttgt tgccaatcaa gcgatctgta agttcttcct tgcttactgt
     6421 cagtcccatc tttttggcct ccatagccat aattttttcg ttgatcagac tttcccaaac
     6481 ctgagtacgc atttgtgaca tttcattttc gttgaaatca tttcttccgt attcaatttt
     6541 ataaacagta ttcatctggt cgattgcttt ctggtaggtt tcgatgtcaa tcttttcacc
     6601 tgctacgtct gctacgtttc gttttgcctg atggaatagg gtggaaccgg aattcaaaaa
     6661 gtcgccaatt acgaatgcta ataaggctaa tccaataatg atagccagta aaactcctct
     6721 gttcctgata gtttgtaatg ttgccatttt attaaaaagt tgataataat attttgataa
     6781 attttaattt taatttgagg gcacgaattt acaaaaaaaa agtgtataaa cgtgcatact
     6841 tacctgattt tttgagaaat gattgttttt agcttgtggt aaaatctgaa aattatttgt
     6901 ttacctgcat tttaaccaat tctattctgt tgttggtcac tttcaggcac ttgaaggtaa
     6961 accggtccac tcgtacaatt tcattcactt tcgggaagtg ttgatgggcg tgtaagatgt
     7021 atccggcaat ggtagagtaa tcttctgaaa caggtatgct gaggccaaac atttgattgg
     7081 cttcctccac ttcaagtctt ccggcaaaca gatattcatt ttcggcagtt ttctctgcgg
     7141 taatatcttt gaagtcgtgc tcgtcttcta tttctccgaa aatttcttcc ataatatctt
     7201 ccaaagttac tattccggct gtgcctccaa attcatcaac cactacagct atactttttt
     7261 tctgctgcat gaaaattttc atcagtttct gagcagccat attttccgga acgataggaa
     7321 tgggtttaat gtgtttcacc cagtcttttg aatgtttgaa catttcggaa gagtgaatgt
     7381 atccaacaat gttgtcaata tcttctttgt aaatcagaat tttcgagagt cctgtttcaa
     7441 taaaggtttg tttgagcgta ttcacgttta ctccatattc atgggctacc atttcggtgc
     7501 gtggaacgta gcaatcgcgc agcttaactt tagagaaatc aagtacattc tgaaagattt
     7561 tcacatcatt gttcaggtct ttgatatctt cattcgcttc caaattttcc tgtagcagat
     7621 aattcaaatc aatacgcgaa aaaacatttt cttctgtgga agctggaatt tcagttttag
     7681 aaaatctgaa aagtttgagc gaaagccagg aaattattcg cgaaaaagga tataaaagga
     7741 agtaaaataa gagcaaaagc catgaaaata tcctcaacca tagatttgga tgaagtctga
     7801 atactgtttt aggcaaaaat tctccgacta tcaccaggat agtggcagca ataataattt
     7861 gaagaatcag gctgaatgca agccccattg aagctaaagg ctctacaagc cacatcgcaa
     7921 taagtactcc gtaagctacc agacttatgt atttgccaac aaataatgtg gaaataaact
     7981 gttgaggatt gttgtagaaa atcgaaataa tataggaatt cagactttta tctttctttg
     8041 ccaactcaaa acgcaatcgg ttggaagcca tataagcagt ctcaataccg gcaaaaaaca
     8101 atatgcctgc cagcgataga attatgtacc caattaagtt caaacctgaa aaattagttt
     8161 aatagagtgt tgaaaatcaa aacattcgtg atattaggat gcaaagatac aatattttag
     8221 gaagttaaac ataataatta gttctaaaaa tgttcatttt gcataaagac aatcctaaaa
     8281 tcgttggaaa tttttagata aactgaatgc ggattgtcaa tctgaattgc tgtgtgccac
     8341 tccccccggt cggctccgcc cgaagtggtg atacaaaaaa caaatctcaa cactttgctc
     8401 tgaaacagat agagttgtga gaatccgact tttaaaatct tggggtaaaa tgaaggatat
     8461 tcaattatta tatttagaaa acccagctgc ttttaaaaag aaaaaaacgg tctgtaaaac
     8521 cgtttttaat ttgtgattca gctgggg
//
LOCUS       DAORKP010000101         8546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1235_length_8546_cov_10.201507, whole genome shotgun sequence.
ACCESSION   DAORKP010000101 DAORKP010000000
VERSION     DAORKP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_8546_cov_10.201507"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLN75_06655"
     CDS             complement(<1..790)
                     /locus_tag="PLN75_06655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRG03831.1"
                     /translation="MLAKIQETADFIKSKVKVMPKTGIILGTGLGNLVTQITDKLEIP
                     YQTIPNFPVSTVEGHSGKLIFGKLGDVDVLAMQGRFHFYEGYDMKQVTFPVRVMKALG
                     IQNLMVSNAAGGMNADFEIGDLMIITDHINVFPEHPLHGKNYKELGVRFPDMSEAYNR
                     EFIAKAKAIAKRNNIRVVEGVYVGTQGPTFETPAEYKYFKIIGGDAVGMSTVPEVIVA
                     NHAGMRVFGMSIITDLGVEGKIVEVSHEEVQQIGNAAQPLMTKIM"
     gene            complement(762..1838)
                     /gene="lpxK"
                     /locus_tag="PLN75_06660"
     CDS             complement(762..1838)
                     /gene="lpxK"
                     /locus_tag="PLN75_06660"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRG03832.1"
                     /translation="MAKPHKNIVFYLLSVLYGIVTEARNRMFDWGILKQHSFDFPIIS
                     IGNLAVGGTGKTPHTEFIIQQLSKNIKIAVLSRGYKRATKGFVLAGKESTAKTIGDEA
                     FQIFSKFPEVTVAVDEKRVRGIGRLMEQCPEIKAIILDDAYQHRHVKAGLSVLLTDYS
                     ALYADDFILPYGNLREHKNNSNRADIIVVTKCPDEFQLEEFSKIERKLNPATHQQLFF
                     SGFEYGNIYPVFSEGKNLSLEKTGEGMAILLVTGIVSPTPLLNYLKNFTTKITTKFFP
                     DHHDFSKNELYDIEKEFEKMTGFKIILTTEKDAARLKSNPYLTEKLKKNLYALPLEVK
                     ISNNEQDKFIQKIQDYVSKNSGNR"
     gene            complement(2018..3781)
                     /gene="sppA"
                     /locus_tag="PLN75_06665"
     CDS             complement(2018..3781)
                     /gene="sppA"
                     /locus_tag="PLN75_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRG03833.1"
                     /translation="MKQFLKFTLATIVGIIITSVIGMLLMFGIIGAIAGSAEKVVKVQ
                     PNSVYELELNGQLIERSKDDPFTEIFSNAFGQDAAKIIGLDDVLANIRKAKVDENIKG
                     IYLKGGMLTGGYASVKEIRDALADFRQSGKFIVAYADTYSQKNYYLSSIADKVYLNTQ
                     GMLEFQGIAANTTFFKNTLDKLGVEMQIVKVGTYKSAVEPYIDTKMSEANKEQVTAFI
                     NSIWKNVLAEISQSRNIPVDSLNRYADAMMTFQPTEMALKNKLVDSLVYADGMNEVFK
                     KLTKQEDFKDIHFISHSDMNKVPHSEKMQKQKVAVIYGVGGIDMGQTDEITSEDLVET
                     INEVKDEKSVKAVVFRVNSPGGSAYGSEQIWHAITELKKVKPVIVSMGDYAASGGYYI
                     SCAADSIIAQPNTLTGSIGIFGTIPNIEGLNKKIGLTHDGVKTNKMSDAISLNRAFTP
                     DERNIMQSYINRGYELFTKRCADGRGMNIDSLKAIAEGRVWTGEDAMRIGLVDKLGGL
                     GDAIKIAAGKAKLENYMVKEFPAKEDIMTRLFKDMNSTVETKILKSRLGDENYRLLKQ
                     IEQAKYFNGIQALMPVNITLN"
     gene            complement(3879..4175)
                     /gene="trxA"
                     /locus_tag="PLN75_06670"
     CDS             complement(3879..4175)
                     /gene="trxA"
                     /locus_tag="PLN75_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRG03834.1"
                     /translation="METFNDIINSGKPVLIDFYATWCGPCKAMSPIIEQVGKEVQGKA
                     RVLKIDVDKNQLLAAKYQIQAVPTFAIFKNGQIVWRQPGGTDKQTLLRTLLSHL"
     gene            complement(4242..4676)
                     /locus_tag="PLN75_06675"
     CDS             complement(4242..4676)
                     /locus_tag="PLN75_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRG03835.1"
                     /translation="MTLVRFTGNPLIDRFFDTDLFDWTSKNYSRTNTTLPSVNVKESD
                     NEFSIEVAAPGFDKTDFKIEVHNDVLTVSSEKQTENETKDESERYTKREFSYQSFSRS
                     FSLPLTADGDKVEAVYDKGILTVSIPKKEEAKPKAPRAIEIK"
     gene            complement(4884..6551)
                     /locus_tag="PLN75_06680"
     CDS             complement(4884..6551)
                     /locus_tag="PLN75_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRG03836.1"
                     /translation="MKKTVVIYCKNTQDYHSFPLGTSLLEIYNSLNITLKYKVVAARV
                     NYKVEDLNFLIYKPKDIEFIDSGTSSGMRVYVRTLSMVLSKAVSELFPHSVIRIEHPI
                     SKGYYCNIENTEEPVTPEMVNRIKTKMQEIIDSNEPVILEEKQTEQVKELFRNGNNNT
                     SKDSLFDSLAEPYLRYYRIGKFIDYYTSILLPSTGYLDVFNLIPYYDGMLLQVPDREN
                     PNDLEEMVQQPKMFEIFKEFLSWNKIMGLNSVGEFNKACLDNRAFDLIKIAEALHEKK
                     VASIADMIQARPEVKVILISGPSSSGKTTFSKRLSVQLMVSGLIPKSISLDNYFVDRE
                     ATPRDENGDWDFESLYALDLDFFHLQMQQLIAGEEIELPFFNFESGKRYFKGEKLKLE
                     KDTLLILEGIHALNPELTKSIPPEALFKIYVSALTTISIDEHNWIPTADTRLLRRIIR
                     DYHYRGYSARDTIGRWSSVRKGEDKWIFPFQENADIMFNSALIFELAVLKRHVMPILE
                     QVPKYCDEYTETHRLLRFLRYFVAINDSEIPPTSLLREFVGGSSFTY"
     gene            complement(6696..7964)
                     /locus_tag="PLN75_06685"
     CDS             complement(6696..7964)
                     /locus_tag="PLN75_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG03837.1"
                     /translation="MTNLAKTYCVLFVFFIGYQALKAENKPFSQLQDTAINVFSGVIT
                     GEKGDKMPYAVLSVEKTNISTVTNADGEFLLKVPKSDMDKNVLVSFLGYENLRIPLAA
                     MMPRGNKYSMRPLQFTLPPLEVISKDPESIIRKMMENKDKNYSNKDMMMTAFYREIIK
                     KRNTNVSLSEAITEVYKRAYNSSLHDMASLYKSRKSTDYNKLDTLVFKLMGGPFNNIY
                     LDVMRYPDYIFSENIFENYEFKFLKTDQINDRIVYVIDFKQLRHIVEPLFYGQLYIDA
                     ETYALARAEFDMDLTNNDEATRIFVRKKPINAKVSTYKAHYLIDYRLNDDLWYYSYSR
                     IDLGLKIDWKKRLLNTYYNSIIEMAATDWSDDMDRRTFRSKDRINPTVVIQNEASGFS
                     DPEFWGEYNIIEPDKSIENAINKIKKQLDK"
     gene            complement(8093..>8546)
                     /locus_tag="PLN75_06690"
     CDS             complement(8093..>8546)
                     /locus_tag="PLN75_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HRG03838.1"
                     /translation="GLALFMTELLQKTLKHSECDENLYFFIESSVCRLDECDEGVGNF
                     HLVFMAQLAGRLGFLPDTTEAPTAQYFDLLNGVFEYSKPAHTHYLIPETGDVFRKLTM
                     MDYSMLSQLPMTRKQRGEMLNHFIEYYKIHLPDFHTLHSVEVLHKLWE"
BASE COUNT         2140 a         1780 c         1831 g         2795 t
ORIGIN      
        1 tcataatttt cgtcatcagc ggttgcgcgg cgttgcctat ttgctgaact tcttcgtgcg
       61 aaacttccac aatttttcct tccactccca gatcggtgat gatggacatc ccgaaaaccc
      121 gcattccagc atggttggct acaattactt caggaacagt actcattcca actgcatcac
      181 cgccaatgat tttgaagtat ttatactcag ccggtgtttc gaaagttggt ccctgagtgc
      241 ccacatacac gccttccact acccggatgt tgttgcgttt ggcaatggct tttgctttag
      301 cgatgaattc acggttgtag gcttcgctca tatcggggaa gcgtacgccg agctccttgt
      361 aattttttcc gtgcagcgga tgttccggga agacattgat gtggtcggta attatcatca
      421 gatcaccgat ttcaaagtcg gcattcatgc ctccggcggc attcgaaacc atcagatttt
      481 ggatacccag cgctttcatc acacgcaccg gaaaggtaac ctgtttcatg tcgtatcctt
      541 catagaaatg gaaacgtccc tgcatggcaa gcacatccac atcaccgagt tttccgaaaa
      601 tgagttttcc gctgtgtcct tctactgttg aaacgggaaa attgggaatg gtttgataag
      661 gaatttcgag tttatctgtg atttgcgtaa ctaagttgcc taaacctgtt cccaaaatga
      721 tacctgtttt gggcataact tttacctttg atttgataaa atcagcggtt tcctgaattt
      781 ttgctaacat aatcttgaat tttttggata aatttatctt gttcgttgtt tgatattttc
      841 acttctaaag gcagtgcata gaggtttttc tttagctttt cagtgagata tggatttgac
      901 tttaagcgcg ctgcgtcctt ctcggtcgta agaattattt tgaatccagt cattttttca
      961 aattcttttt caatatcgta cagttcattt tttgaaaaat catgatgatc cggaaaaaat
     1021 tttgtcgtga ttttagtggt gaaattcttc agataattca gcaagggagt tgggctcaca
     1081 atgcctgtaa ccagcaaaat ggccattccc tcccctgttt tttctaatga caagttttta
     1141 ccttccgaaa aaaccggata aatatttccg tactcaaacc cggagaaaaa caactgctga
     1201 tgtgttgccg ggtttaattt tcgctcaatt ttgctgaatt cctcgagttg gaattcatcg
     1261 ggacattttg ttaccacgat gatatccgcc cggttgctgt tatttttatg ctcgcgcagg
     1321 ttgccataag gcagtataaa atcatctgca tacaatgcgg agtaatcggt cagcaaaacg
     1381 gacagtccgg cttttacatg acggtgctga tacgcgtcat cgagtataat cgctttaatt
     1441 tcagggcatt gctccatgag ccgtccgatt cccctcacac gtttttcgtc cactgctact
     1501 gtcacctccg gaaatttgct gaaaatctga aatgcctcgt cgcctatggt tttggcggtg
     1561 ctttcttttc cggcaagaac aaatcccttt gtcgcacgtt tgtatccgcg gcttaataca
     1621 gcaatcttaa tgtttttgct taattgttgt ataataaatt cagtgtgcgg cgtttttcct
     1681 gtgcccccca ctgccagatt tccgatgctg atgatgggaa aatcaaatga atgctgtttc
     1741 aaaattcccc aatcaaacat ccggtttcgt gcctctgtaa cgataccgta aagcaccgaa
     1801 agaaggtaaa atacaatatt tttgtgcggc tttgccattg tatctacaaa gataaaaaat
     1861 gttttcgtat tttgaacgag gtgttggatg gtgagaactg agttttctga aattcagttt
     1921 tataagttcg cagattggat tttcggcttg atacaaatag aaaggtatcg caaacatgct
     1981 gcgatacctt tctttgaatg attttcaagt ctccgtatta gttcagcgtg atattcacgg
     2041 gcatcagtgc ctgaattccg ttgaagtatt tggcttgttc aatttgtttg agcagtcggt
     2101 agttttcgtc tccgagcctg gatttcagta ttttggtttc taccgtggag ttcatgtctt
     2161 tgaacagacg ggtcatgata tcttctttgg cggggaattc ttttaccatg taattttcca
     2221 gttttgcttt tccggcggct atttttatgg catcgccgag tcctccgagt ttgtccacca
     2281 atcctattct cattgcatct tcaccggtcc atacacgccc ttcggcaatg gctttcagcg
     2341 agtcgatatt cattccgcgg ccgtcggcac agcgctttgt gaacagctca taaccgcggt
     2401 tgatgtagct ttgcattatg tttcgttcat cgggtgtgaa tgcgcggttc agagagatgg
     2461 catcgctcat tttgttcgtt ttcactccgt cgtgtgtcag accgattttt ttgttcagtc
     2521 cttcaatatt cggaatggtt ccgaaaattc ctatcgaacc ggtcagtgta tttggttgtg
     2581 caatgatgct gtcggcagcg cacgagatgt agtaaccgcc cgaagctgca taatcgccca
     2641 tagaaacgat taccggtttc acttttttca gttcggtaat cgcgtgccat atttgttccg
     2701 atccgtaagc gctgccgccc ggtgaattta cccggaaaac cacggctttg acagattttt
     2761 catctttcac ttcgttgatg gtttctacca ggtcttcgga agtaatttcg tcagtctgac
     2821 ccatgtcaat gcctcccacg ccgtagataa ctgcaacttt ttgtttttgc attttttcag
     2881 agtgaggtac tttgttcata tcggagtgcg aaataaaatg aatatccttg aaatcttcct
     2941 gttttgtgag ttttttgaat acttcattca tgccatcggc atacacaagc gagtcaacca
     3001 gcttattttt cagtgccatt tcggttggtt gaaaagtcat catagcatcg gcgtacctgt
     3061 tgagcgagtc cacggggatg ttgcgcgatt gagaaatttc tgccagcaca tttttccaga
     3121 tggagttgat aaaagcggtt acctgctcct tgttggcttc actcattttg gtgtcaatgt
     3181 aaggctctac ggcagatttg taggttccca cttttactat ttgcatttcc acacctaatt
     3241 tatccagcgt atttttgaag aaagtggtgt tggcagcaat tccctgaaat tcaagcattc
     3301 cctgcgtgtt cagatacact ttgtcggcta tggatgagag gtagtagttt ttctgactgt
     3361 acgtatcggc gtaagccaca ataaacttac ccgattgcct gaaatcggcc aaagcgtcac
     3421 gtatttcctt aacagaggca tagccacctg tcagcatgcc gcctttcagg taaattcctt
     3481 ttatgttttc gtcgactttc gctttgcgga tgttggccag cacatcatcc agcccgatga
     3541 ttttagctgc atcctgtccg aatgcatttg agaaaatctc ggtgaacggg tcgtcttttg
     3601 agcgttcaat cagctgaccg ttcaactcaa gttcgtaaac agaattaggc tgtactttta
     3661 caactttttc ggcagaaccg gctatagctc cgattattcc aaacatcaat agcattccga
     3721 taacggatgt aatgataatg ccaacaattg tggccagagt aaatttaaga aattgtttca
     3781 taggttttgt gtttcaataa ttgatggtgc aaagaaacga aaaaaaaggg aaattaattt
     3841 gtttagtaac aaatatcaac cgaaaaacat tgattttctc agagatgcga caggagtgtt
     3901 ctcagaagtg tttgtttgtc ggttccgccg ggttgcctcc acacgatttg gccgtttttg
     3961 aaaatggcaa atgtaggtac agcctgaatc tggtatttgg cagccagcag ctgattttta
     4021 tccacatcta ttttcagcac gcgtgctttg ccctgcactt ctttgcccac ttgctctatg
     4081 atgggcgaca tggctttgca cggaccgcac caggtagcgt aaaagtctat cagaacgggt
     4141 ttcccggaat taattatatc gttgaatgtt tccatgaaag tatgatataa gaaaaaggga
     4201 agacgaactt tccgtcctcc ctttaacagt tagaaattca gttacttaat ctcaatagcc
     4261 cgtggagctt tcggtttggc ttcttctttt ttcggaattg aaacagtcag aatacctttg
     4321 tcgtatacgg cttccacttt gtcgccatca gcggtaagtg gtaaactgaa cgaacggctg
     4381 aacgactgat agctgaactc gcgttttgtg taacgttcgc tttcgtcttt ggtttcgttt
     4441 tcagtttgtt tttcagacga aaccgtcagc acatcgttgt gaacttcaat tttgaagtca
     4501 gttttatcaa atcccggtgc agctacttca atgctgaatt cgttgtcact ttctttcaca
     4561 tttaccgaag gtaatgttgt gtttgttctg gaatagtttt ttgaagtcca gtcgaataaa
     4621 tcggtgtcga aaaaacggtc aatcaacgga ttgcctgtaa atcttacgag tgtcatagtt
     4681 gttttccttt cttatttttt agtttgttta atgatttgtt tttttttgtc ttacgacctc
     4741 attaatacaa ataaaatacc aacaggaaaa aactgacaaa atggcatgtt tgaaaacgaa
     4801 tattattatt ccaaatagtt aaacgctgtt tccgatgccg aggatccctt ttgtgagtat
     4861 gacaaaatga ccgatatatt tttttaatac gtaaaactgc taccgcctac aaattcgcgc
     4921 aggagcgatg tgggtggaat ttcgctgtcg ttgatggcta caaagtaacg caaaaaccgc
     4981 agcaaccggt gagtttcggt atattcatcg caatatttcg gcacttgttc caggatgggc
     5041 attacgtgac gtttcagcac ggcaagttca aaaatcagtg ccgagttgaa cataatgtcg
     5101 gcattttcct ggaaagggaa aatccatttg tcctctcctt tacgcacgct gctccatcgt
     5161 ccgatggtat cgcgggctga gtatccgcgg taatggtagt cgcggatgat gcgtctcagc
     5221 aaacgtgtgt cggctgtggg tatccagttg tgttcgtcga tggaaatggt ggtgagtgcc
     5281 gatacgtata ttttgaacaa agcttccggc ggaatgcttt tggtgagttc cggattgagt
     5341 gcatggatgc cctccaggat gaggagcgta tctttttcca gtttcagctt ttcgcctttg
     5401 aaatatcgtt ttccgctttc gaagttaaaa aacggcagtt cgatttcttc tccggcaatt
     5461 aattgctgca tctggaggtg aaaaaaatcg aggtcgagtg catagagcga ttcaaagtcc
     5521 cagtcgccgt tttcatcgcg gggtgtagct tcgcggtcca caaagtaatt gtcgagcgat
     5581 atgcttttgg gtatgagtcc gctcaccatc agctgcacgg acaatcgttt gctgaaagtg
     5641 gttttacccg acgatgaagg ccccgaaatc agtatgactt tcacctcggg ccgggcttgt
     5701 atcatatcgg ctatcgatgc tacttttttc tcgtgcagcg cttcggcaat ttttatcaga
     5761 tcaaaagcgc ggttgtccag gcaggctttg ttgaattcac caaccgaatt cagccccatg
     5821 attttattcc agctcagaaa ttccttgaat atttcaaaca ttttgggctg ttgcaccatt
     5881 tcctcaagat catttggatt ttctctgtcg ggtacctgca gcagcattcc gtcgtagtag
     5941 ggtatcaggt tgaacacatc cagatatccg gtagagggca gcaggatgct tgtgtaataa
     6001 tcgatgaatt ttccgatgcg gtaatagcgc aggtagggct cagccaggct gtcgaaaagc
     6061 gaatctttcg aggtgttgtt gttgccatta cggaataatt cctttacttg ttcggtttgt
     6121 ttttcttcca gaatgacagg ttcattgctg tcaatgattt cctgcatttt tgtctttatc
     6181 cggttcacca tttcaggggt aaccggttct tccgtgtttt cgatgttgca ataatatcct
     6241 ttggagatgg ggtgctcaat ccggataact gaatgtggaa aaagctccga aactgctttt
     6301 gagagcacca tagagagtgt tcgaacgtaa acacgcatgc ccgatgaagt gccgctgtcg
     6361 ataaattcga tgtctttcgg tttgtagatt aaaaaattca ggtcttctac cttgtaattt
     6421 acccgggcag caaccacttt gtattttagc gtgatattca gcgaattgta tatttcaagc
     6481 aacgatgtgc ccaatgggaa ggaatggtaa tcctgcgtgt ttttgcaata gatgacgacg
     6541 gtttttttca taaaaaaagt gatttgtttt ttcgaatccg actgtctgtg aatcgttcgg
     6601 ttgagtttcg tttttgcaca taataataat tccaccacaa agttagtgga attattataa
     6661 aaagagttga tcctggaagt caattacttt ttttgctatt tatccagctg ttttttgatt
     6721 ttgttgatag cgttttcgat ggatttatcg ggttcaatta tgttgtattc tccccaaaac
     6781 tcgggatccg aaaatcccga tgcttcattt tgaatcacta cagtaggatt aattcggtct
     6841 ttggagcgga aagttcttct gtccatatcg tccgaccaat cggtggcagc catttcgatg
     6901 attgaattgt aataggtgtt taaaagccgt tttttccagt caattttcaa tcccagatca
     6961 atccggctgt aagagtagta ccataaatcg tcattcaatc tgtaatcaat cagataatgt
     7021 gccttgtagg tactcacttt ggcattaatc ggttttttgc gtacaaatat ccgggtagct
     7081 tcatcgttgt tagtcaggtc catatcaaat tcggcccttg ccagtgcgta agtttcggca
     7141 tcaatataca gctggccgta aaacaaaggt tccacaatgt gtctgagttg tttgaagtca
     7201 atcacataaa caatacgatc gtttatctgg tctgttttga ggaacttaaa ttcataattt
     7261 tcaaaaatat tttccgaaaa aatataatcc ggataacgca ttacatccag atagatatta
     7321 ttgaaaggcc ctcccatcag tttgaaaacc aacgtatcga gtttattgta atcagtgctt
     7381 ttgcgggatt tatacagtga tgccatatcg tgcaggctgc tgttgtaagc ccgtttgtaa
     7441 acttcggtaa tggcttccga aagtgatacg ttcgtgtttc tctttttgat aatctcacgg
     7501 tagaatgccg tcatcatcat gtctttgttg ctgtaattct tgtccttgtt ttccatcatt
     7561 ttccggatga ttgactccgg gtcttttgat atgacttcaa gcggtggaag tgtaaactgg
     7621 agtggcctca tcgagtattt gtttccacga ggcatcattg ctgccagcgg aatacgcaga
     7681 ttttcgtacc cgagaaatga aaccagtacg tttttgtcca tgtctgattt tggtactttg
     7741 agtaaaaact caccatccgc attggttacg gtagagatat ttgttttctc aaccgaaagc
     7801 actgcgtaag gcattttgtc gcctttttca cctgtaatta cacctgaaaa tacatttata
     7861 gcagtatcct gcaactgtga aaatggttta ttttcagctt ttagggcctg atatccgata
     7921 aaaaaaacaa acagaacgca gtatgttttc gcgagatttg tcatttgaat tgatcgaatt
     7981 gatttcatga ttgtgtattt tgttaaaaaa gtcagattaa agttgttttt ttttatttgt
     8041 aaaatttcat tccataatac aaaacaaaca tacggataaa atgttgaaag tattattccc
     8101 aaagtttatg aagcacctcc accgagtgaa gtgtgtgaaa atcaggcagg tgaattttgt
     8161 aatattcaat gaaatgattc agcatctcac ccctttgttt tcttgtcatg ggcagttggc
     8221 tcagcatgct gtaatccatc atcgttagtt ttctgaaaac atcccctgtt tcgggtatca
     8281 gataatgtgt gtgagccggt ttgctatatt caaaaacacc gttcaacaga tcaaagtact
     8341 gagcagttgg agcttctgtt gtatcgggta aaaatccaag ccgcccggca agctgcgcca
     8401 tgaaaaccag gtgaaaattg cccacgccct catcacattc atccagcctg cagaccgacg
     8461 attcgatgaa aaagtacaaa ttttcgtcac attccgaatg tttcagcgtt ttttgcagca
     8521 attcagtcat aaacagcgcc agccca
//
LOCUS       DAORKP010000102         8451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1264_length_8451_cov_8.893283, whole genome shotgun sequence.
ACCESSION   DAORKP010000102 DAORKP010000000
VERSION     DAORKP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_8451_cov_8.893283"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2815
                     /locus_tag="PLN75_06695"
     CDS             <1..2815
                     /locus_tag="PLN75_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRG03839.1"
                     /translation="PNPPLVPEEFNPTGTYRREFEIPDSWKGEDIILAFDGIKGASYI
                     YFNGEFLGMTKDSKLPARFDISTIAKAGKNVIALQTYRWSSASYLECQDFWRLSGIER
                     DVYVYTRPKMHIADFFAKPLLDKNYENGVFNLDVQVKNSNNKPTTFTLNYEIADATGK
                     SIAKDSKTLTAEKSVSEFSFNEKNIPGVKKWSAESPDLYSLVIELKDNNGKVLEATAV
                     KIGFRTAEIKNKQFMINGKPVLVKGVNIHEHNEFTGHYVSEELMRKDFELFKKYNVNT
                     ARTCHYPQPELFYKLADEYGIYVIDEANIEAHGMGYGLHVGGTLGNNPLFLEDHLNRT
                     TGMVERDKNHACVVTWSLGNESGNGYNMYETYRWIKNRDTSRPVQYERAGLEWNTDIF
                     CPMYDRPDEIERYAQNPASNRPLILCEYAHAMGNSLGNFTEYWDVIRKYPLLQGGCIW
                     DWVDQGLAQTDKDGVKFWAFGGDFGPKGTPSSGDFCCNGMVLPDRSLKPQTLEMAKVY
                     QNVWFKNFDYKKESVDIYNENFFVDLSQYDISYEIKSNGKTLKTGKINVQAEPQETKT
                     VKIPGIAGLFKDGQQTTIQFFVKQNKATQAIPAGWLVAKDQFIVNEYPKMQITNNKKP
                     VKLEDNATYMKVSGSNFEAAFDKSSGIMTSYKFKGTEYISDGFGLRPFFARAPLDNDY
                     GAQLPRKLKAWKDASYAQPKAVNFSHEVTADNATAITLTYEYPGVNATWKVKYTINEN
                     GKISVDNHFDATRNQLPLIFRVGMRMQLPGSVVNAEYYGRGPLENYADRKTSMFVDRY
                     KSPIIDMAAKYVFAQENGHHVDTRWLALTQKSGAGLLFASDKNFEFNVSNYLLETISN
                     GDDWNNDAPVGTAPVNKHINAYKPSDKVDLFIDYRMQGVGGNNSWGELPLEQYRIVPK
                     NTDISYSFTIIPVNNTKEIDKIF"
     gene            complement(2869..3585)
                     /locus_tag="PLN75_06700"
     CDS             complement(2869..3585)
                     /locus_tag="PLN75_06700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRG03840.1"
                     /translation="MSLIAASVLPVIILMIFFYSRDKFEKEPPKTLLKAFSGGVLSAF
                     LAVILAVSMSFPLPETSSAGYAAFIQSFWEAAVPEEISKFALLYLFIWRDKNFNEYYD
                     GILYAVFVSLGFACVENFLYLMEHGMSIAMSRALLAVPLHALCGVIMGYYFSLARFTG
                     RKISNLLKAVLLAILAHGVYDFILFFLQPYVELSPAVGLLSTVLVFGFVGLLWKISLG
                     KIRNHVNNSVFRYNKPDELV"
     gene            complement(3928..5130)
                     /locus_tag="PLN75_06705"
     CDS             complement(3928..5130)
                     /locus_tag="PLN75_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRG03841.1"
                     /translation="MKRTFVTWIVLLGIFVSVSSQTVVVGDSAELSMITCSPGPLSYE
                     KFGHTALRYFDPATGVDLVFNYGIFDFDQPNFYTKFIKGETYYMLGVYKTGQFLPQYA
                     ERNSQVTEQVLNLTKAEKQQLLDALFVNYLPQNRNYLYNFVFDNCATRPRDKVLELFR
                     GKQILYNLSEDNRSFREWVAAYTGEKSWLMFGIDLLFGKDADRIATKWETMFLPEVLS
                     KELSEVQVVDNDTLTRNLFKSESILINKIPEVESVNYLKLPIVATSLLLLIGLLITFY
                     ENKKKKYFKLVDSLLLIITGIAGIILFYLMFFSIHPLVKSNFNLLWCNPINILVGIIL
                     WIRKLAKVVHVMQGINILLFLTVLIVGAVGFQILNPAFLPLIVLLLIRSFWWLHNRHK
                     LDFTHENL"
     gene            5381..6082
                     /locus_tag="PLN75_06710"
     CDS             5381..6082
                     /locus_tag="PLN75_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRG03842.1"
                     /translation="MTKRHNSSLKPIEDVFSFWDVMTPDERNFIQNTYTVQHYRKNEV
                     IQMEGDIPTHLMVLASGKVKVYKTGFGNHPQIVRMLKPGENFGYRAIIANEPNSTTVT
                     AFEASSVYMISGEIFLSILRHNNAFCYRFLEEMSFDLAESDARTVNLTQKHIRGRLAE
                     SLLFLKENYGVEEDGATISIYLSREDLANLSNMTTSNAIRTLASFVNDEMIAMDGRKL
                     KIIDEERLRKTSKLG"
     gene            6143..8356
                     /locus_tag="PLN75_06715"
     CDS             6143..8356
                     /locus_tag="PLN75_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03843.1"
                     /translation="MTPTEIHSTYKKTLTYLTEGRLKNAFEKITELNTELQSAAHEQN
                     IENMQTSYRYLLQYFMEGANDPERKTIYNKLIARTFTAAAEMRDELLTRDSTNFEFTQ
                     KRYFPYKEQLSPANIKEWINRKEKLAELHEINTGDIEKENRIESEFENAVRYLFNYFW
                     LKTNYDSAGIMEIYNRVMDDEFTDSVAKSMLVSAITLNLWRTFNENKLLMLLDACNSA
                     DINTQQRALVGLCFILAKYNRFLPYFPSVRNRLILLADNDILIEKLQNIIIQIIGTTE
                     TEEISKKLQEEIFPELMKLRPMMDEGLTGDINPLNTDEWGEINPDWQEMIENSGVSDK
                     IQEFAEMEMSGADVYMSTFSMLKSFPFFQEFSNWFLPFYTDNQAVKPLFTSDEKSLIS
                     VFVNSNVMCNSDRYSFCMSILRMPDMQRNMMKQSFSQAAEQMEEMNKDEALITPNVQA
                     KTISKHYIQDLFRFFKLNTHRNDFADMFQTSLIMHKTYLFDMLSMDDEIKKNIAEYYF
                     SKKLYIQALELFGEIEKEGESSASLYQKMGYAYQQNSQLTLALQAYKKADIIQPDDFW
                     TNRKIALCYRLLGDNENALSTYRHADFIKPNNLSVQLQVVNCLKELKRYDEALKELSK
                     LYKEYPENQKVLRTTMWVAFNAKNIAQASYFASILLDTADVTAYDRMIAGHIDWCMNK
                     NKEAREKYDYVLKMLDNNWDMFVNMFRQDSELLNANGVDNEEIPLILDSIAFQFF"
BASE COUNT         2779 a         1880 c         1753 g         2039 t
ORIGIN      
        1 accgaatccg ccactcgttc ccgaagagtt taaccccaca ggcacttatc ggagagaatt
       61 tgaaattccc gatagctgga aaggcgaaga tattatcctc gcgttcgacg gcatcaaagg
      121 cgcgtcgtat atttatttca atggcgaatt tctcggaatg accaaagaca gcaagctccc
      181 ggcccgcttt gatatatcga ccatagccaa agccggcaaa aacgtgattg cgcttcaaac
      241 ataccgctgg agcagtgcct cttacctgga atgtcaggac ttctggcgtc tgagcggcat
      301 tgagcgcgat gtgtatgtgt acacacgccc caaaatgcac attgccgatt tttttgcaaa
      361 accattgctg gataaaaact acgaaaacgg agttttcaac ctggatgttc aggtaaaaaa
      421 cagcaacaat aagcccacaa catttacact taactatgaa atagcagatg ccactggcaa
      481 gtcaatagcc aaagattcca aaacattaac agctgaaaag agtgtatcgg aattcagttt
      541 taacgaaaaa aacatacccg gagtaaagaa atggtcggca gaatcgcccg atctttattc
      601 cctggttatc gaattgaaag acaacaacgg aaaagtgctg gaagcaacag ctgttaaaat
      661 cggattccga acagccgaaa tcaagaacaa gcaattcatg atcaacggga aaccggtact
      721 ggtaaaaggg gtgaatattc atgagcataa cgaatttacc ggacactatg tatcggaaga
      781 actgatgcgg aaagatttcg aacttttcaa aaaatacaac gtaaatacag cccgcacctg
      841 tcactatcca cagccggaat tattctacaa actggccgat gaatacggta tttatgtaat
      901 cgacgaagcc aacattgaag cccacggaat gggatatggt cttcatgtgg gtggtacgct
      961 gggtaataat cctcttttcc tcgaagacca tctgaatcgt accacaggaa tggtggaacg
     1021 ggataaaaac catgcttgcg tagtgacctg gtcgctgggc aacgaaagcg gcaacggcta
     1081 caacatgtac gaaacttacc gctggataaa aaaccgcgac acctcacgcc ccgtacaata
     1141 tgaacgggca gggctggagt ggaataccga tattttctgc cccatgtacg accgcccgga
     1201 cgaaatagaa cgatatgctc aaaatccggc ttcaaaccgc ccgctcattc tctgtgaata
     1261 tgctcatgcc atgggcaata gcctgggtaa ttttaccgaa tactgggacg ttatccgaaa
     1321 atatccactg cttcagggtg gttgcatctg ggactgggta gatcaggggc ttgcacaaac
     1381 cgataaggat ggcgtaaaat tctgggcttt cggaggcgat tttggtccga aaggaacacc
     1441 atcttcaggc gatttctgct gcaacggaat ggttctgccc gaccgcagcc tgaaaccaca
     1501 aacactcgaa atggccaaag tgtatcaaaa tgtatggttc aaaaattttg attacaaaaa
     1561 agaaagcgtg gatatctaca acgaaaattt ctttgttgat ttgagtcagt acgacatttc
     1621 gtatgaaata aaatcaaacg gaaaaacgct gaaaaccgga aaaatcaacg tacaggctga
     1681 acctcaggaa accaaaacgg taaaaattcc gggtattgca gggcttttca aagacggcca
     1741 gcaaaccacc attcagttct ttgtaaaaca aaataaagct actcaggcaa ttcctgccgg
     1801 ctggttggtg gctaaggatc agttcattgt gaacgaatat ccgaaaatgc aaatcacaaa
     1861 caacaaaaaa ccggtaaaac tggaagacaa cgccacttac atgaaagtaa gcggaagcaa
     1921 ctttgaagct gcttttgaca aatcttcggg catcatgact tcatataaat ttaaaggcac
     1981 agaatacatc agcgatggat tcggacttcg tccgtttttt gcacgcgcac cgctcgacaa
     2041 tgattacggc gctcagcttc cccgcaaact caaagcctgg aaagatgcca gctatgccca
     2101 accaaaagct gtgaatttca gccacgaagt gactgctgac aatgcaaccg ccattacgct
     2161 gacttacgaa tatcccgggg taaatgctac atggaaagtg aaatacacaa tcaatgaaaa
     2221 cggcaaaata tctgtcgaca accatttcga tgccacacgc aaccagcttc cgcttatttt
     2281 ccgcgtggga atgcgaatgc agctgcccgg aagcgtggta aatgctgaat actacggacg
     2341 cggaccactg gaaaactatg ccgaccgcaa aacttccatg ttcgttgaca gatataaatc
     2401 gccaatcatt gacatggcag caaaatatgt attcgcacag gaaaacggcc atcacgtaga
     2461 tacccgctgg cttgcgctga cacagaaatc aggcgccggt ttgctctttg cttctgacaa
     2521 aaattttgaa ttcaacgtat cgaactacct gctcgaaacc atctccaatg gtgatgactg
     2581 gaacaacgat gcacccgtag gcacagctcc ggtgaataaa catatcaatg cctacaaacc
     2641 atcggacaaa gtagatttat ttatcgacta ccgcatgcag ggcgtgggtg gcaacaacag
     2701 ctggggagaa ttgccgcttg agcagtatcg gattgtaccg aaaaatacag atatttcata
     2761 tagttttaca attattccgg taaataatac gaaggaaatt gataaaatat tctgatctga
     2821 cattattctg aaaaaaaagg atgctcccgc gcatcctttt tttttgtttc aaaccagttc
     2881 atccggttta ttgtacctga acacagaatt gttcacatga ttcctgattt tccccagcga
     2941 aattttccaa agcagaccaa caaatccgaa tacaagcact gtggacagaa gtccgactgc
     3001 cggactcagt tcaacgtaag gctggagaaa aaacaggata aaatcataca cgccgtgagc
     3061 cagaatggcc agcaaaactg ctttcaataa attggaaatt ttccggccgg tgaaccgagc
     3121 cagtgaaaaa taatagccca taatcacccc gcacaaagca tgcagcggaa ctgccagcaa
     3181 ggctcgtgac atggctatac tcatgccgtg ttccataaga tacaggaaat tctccacgca
     3241 ggcaaaaccc agcgaaacaa aaacggcata gagaatacca tcgtaatatt cattgaaatt
     3301 cttatcgcgc cagataaaaa gatagagcag cgcaaacttg cttatttcct ccggaacagc
     3361 agcttcccaa aatgactgta taaatgcggc atatccggcc gaagaagttt cgggcaaagg
     3421 aaatgacata ctcacagcca gaataacggc taaaaaagcc gaaagaactc ctcctgaaaa
     3481 tgctttgagc aacgttttgg gcggttcctt ctcaaattta tcgcgggaat agaagaatat
     3541 catcaggatg atgaccggaa gtactgaggc tgcaattaaa ctcataacga tgtttttgcc
     3601 gtaaatatac tgtaaaaaat tgattcgtgt attcaaccgg atggatgtaa atgcatttat
     3661 aaactacatt tttcacattt acaaaaatga attatatcaa taaaatcgct gagattcttc
     3721 gggaacggtt ttgaccgtgc ttaattcagt aaagcaaaat gctgctttaa gcgtctgttt
     3781 gctaactcag aagtcgagat aaactctatt tttattttac aacaacttac tgagtcctga
     3841 tccaactttt tattttcaaa gaaaacggaa tgaactttgg cagatcattc cgtatgattt
     3901 atagaaagtg gcaggtgatg aaagcgatca aaggttttcg tgagtaaaat ccagtttatg
     3961 cctgttgtgc agccaccaga acgatctgat caacagaagt acaatgagag gtaaaaacgc
     4021 cggattgaga atctgaaaac ccactgctcc cactatcagg actgtgagaa acagcaagat
     4081 attaatgccc tgcattacat ggacaacttt cgccagcttc ctgatccaca gaataatacc
     4141 caccagaata ttgataggat tacaccaaag caaattgaaa ttggacttta cgagcggatg
     4201 aatggaaaaa aacatcagat aaaacagaat gattccggca ataccggtaa tgataagcag
     4261 cagagagtca accagtttaa agtacttttt ctttttattt tcgtaaaacg taattaacag
     4321 gccgataaga agcagcaatg aggtggctac aataggaagt ttcagataat taaccgattc
     4381 aacttcgggt attttgttga taagaatgga ttcgcttttg aacagattac gtgtaagagt
     4441 gtcattatca acaacctgaa cttcactcaa ctccttgctg agcacttcgg gcaaaaacat
     4501 ggtttcccat ttggtagcta tccggtcggc atctttaccg aaaagcaaat caatgccgaa
     4561 catcagccag cttttctcac ccgtataagc agccacccac tcgcgaaacg agcggttgtc
     4621 ttccgaaaga ttataaagaa tctgcttgcc tctgaacaac tccaacacct tatcacgcgg
     4681 tcttgtggca caattatcaa acacaaaatt gtagaggtag tttctgtttt gcggcaggta
     4741 gttcacaaaa agcgcatcga gcaactgctg cttttcggct ttggtgaggt tgagcacctg
     4801 ctctgtgacc tgcgaattgc gctcggcata ctgcggcaga aattgcccgg ttttataaac
     4861 gccaagcatg taatacgttt cacccttgat aaatttggta taaaaattgg gttgatcaaa
     4921 atcaaaaatg ccgtaattga acaccagatc gacaccggta gccggatcga aataacgcag
     4981 tgcggtatga ccgaattttt cgtaggaaag cggtcccggc gagcaggtga tcatgcttaa
     5041 ctcagccgaa tcgcccacaa caacggtctg cgaacttacc gaaacgaata ttcccaaaag
     5101 aacaatccag gtaacaaaag tccttttcat aatctttttt tttgtgacaa cgacaaaaat
     5161 acaatttttc tttcaaacta tgcatcaatc attaaagtga ttttttccga ttgaataaat
     5221 gattttagaa ttaaacaacc acgaggataa aaaaaagtgt taattataag aaattcagga
     5281 acagaacaag tcactttttt aaataaattt gtcggtaatg tgaatttttc gttgtaaatt
     5341 tgtgctgtaa aacattgttt tcaaacataa agagtgcacc atgaccaaaa gacataattc
     5401 atcgttgaaa cccattgaag atgttttttc tttttgggac gtaatgactc ccgatgaaag
     5461 aaattttata caaaacacgt acaccgttca gcactaccgg aaaaatgaag tcatacagat
     5521 ggaaggcgac atccctacac acctcatggt gctggcaagc ggcaaggtga aagtatataa
     5581 aaccggtttc ggcaatcacc cgcagatagt gagaatgctc aaaccgggcg aaaacttcgg
     5641 ataccgcgcc atcatagcca atgagccgaa cagcaccacc gtgacggcat ttgaggcatc
     5701 atcggtgtac atgatcagcg gagaaatttt tctttccata ctccgccaca acaatgcttt
     5761 ttgctaccgg tttctggaag aaatgtcatt cgatctggca gaatcggatg cccggactgt
     5821 caatttgaca caaaaacaca tcaggggaag attggcagaa tctttgctct ttctgaaaga
     5881 aaattacggt gtggaggaag acggtgccac aataagcatt tatctttcaa gagaagacct
     5941 ggcaaacctc tcaaacatga ccacctccaa tgccatccgg acactggcaa gttttgtgaa
     6001 cgacgaaatg atagccatgg acggacggaa actgaaaata attgatgaag aaagactccg
     6061 gaaaacaagc aagttaggtt aacacgtatc ttttgttttt cagtccgttt ctcatccgaa
     6121 aaataacaca cgaaaacaaa agatgacacc taccgaaata cacagcacat acaaaaaaac
     6181 ccttacatac ctcacagaag gaagactcaa aaatgctttt gagaaaatta ccgagctgaa
     6241 caccgaactt cagtcggctg cacacgagca gaacatcgaa aatatgcaga caagctacag
     6301 atacctgctg caatacttca tggaaggggc gaacgatccg gaacgaaaaa caatttacaa
     6361 caagctgatt gccagaacat tcacagctgc tgctgaaatg cgcgacgagc tgctcactcg
     6421 cgattccacc aatttcgaat ttacacagaa acgctacttt ccctacaaag agcaactaag
     6481 ccccgctaac atcaaagaat ggataaaccg gaaagaaaaa ctggccgaac tgcacgaaat
     6541 aaatacaggt gatattgaaa aggaaaatcg gatagaatct gaatttgaaa atgcagtgcg
     6601 ctacctcttc aattacttct ggcttaaaac caactacgat tcagccggaa taatggaaat
     6661 atacaaccgc gtaatggacg atgaattcac cgattcagta gccaaatcca tgctcgtatc
     6721 ggccattaca ctcaacctct ggcgaacttt caacgagaat aagctgctca tgctcctcga
     6781 tgcctgcaac tcggctgata ttaacaccca gcagagagct ttggttggtt tgtgttttat
     6841 tctggccaaa tacaacaggt ttctacccta tttcccatcc gtcagaaacc ggctgatact
     6901 cttggccgac aacgacatac tgattgagaa actgcaaaac atcatcatac aaatcatcgg
     6961 aaccaccgaa actgaagaga tttcgaaaaa gctgcaggaa gaaattttcc cggaactcat
     7021 gaaactccgc cccatgatgg atgagggact aacgggcgat atcaatccgc taaacacgga
     7081 cgaatggggc gaaattaatc cggattggca ggaaatgatt gaaaacagtg gtgtttcgga
     7141 caaaattcag gaatttgccg aaatggaaat gagcggagcc gacgtgtaca tgagcacttt
     7201 ctccatgctg aagtcattcc cgtttttcca ggagttttcc aactggtttt taccgtttta
     7261 caccgacaat caggctgtaa aaccactctt cacgagcgac gaaaaatcgc tcatcagcgt
     7321 ctttgtgaat tcaaacgtga tgtgcaactc cgaccgttat tcattctgca tgagcatact
     7381 ccgcatgccc gacatgcaac gcaacatgat gaaacaatcc ttcagccagg ccgccgaaca
     7441 gatggaagaa atgaataaag acgaagccct gataacgcca aatgtgcagg ctaaaaccat
     7501 ttcgaagcat tacattcagg atttattccg ctttttcaag ctgaatacac accggaacga
     7561 ctttgccgat atgtttcaga cctcgttgat catgcataaa acctatcttt tcgacatgct
     7621 ttccatggat gatgaaatca agaaaaacat tgccgaatat tatttttcga aaaaactgta
     7681 cattcaggcg ctcgagcttt tcggggaaat agagaaagaa ggcgaatcgt cggcatcgtt
     7741 gtaccagaaa atgggttatg cctatcagca gaattcacag ctcacactcg cgctgcaggc
     7801 ttataaaaaa gcggacatta ttcagcccga cgacttctgg acaaaccgga aaatagcctt
     7861 atgctaccgg ctgcttggcg acaacgaaaa tgcgctgagc acctaccggc atgccgactt
     7921 catcaagccc aacaatcttt cggtgcaact gcaggttgtc aattgcctga aagaactgaa
     7981 aagatacgac gaagcgctga aagaactctc gaaactctac aaggaatatc ctgagaatca
     8041 aaaggttttg cgtaccacca tgtgggtggc tttcaatgca aaaaacatag ctcaggcaag
     8101 ctatttcgca tccatattgc ttgacacagc agatgttacc gcttacgacc gaatgattgc
     8161 gggtcatatc gactggtgca tgaataaaaa caaagaagca agggaaaaat atgactacgt
     8221 gttgaaaatg cttgataata actgggatat gtttgtcaac atgttcaggc aggacagcga
     8281 gctgctcaat gccaacggtg tggacaacga ggaaattccg ctgattttgg actccatagc
     8341 gtttcaattt ttttaaataa ttgatatgtt gatgagtttt tatgtaatat aatttgtatt
     8401 tatatgtttt ttcgtacttt tgtcaacaaa catttaaata aaaaaatatg a
//
LOCUS       DAORKP010000103         8372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1289_length_8372_cov_8.061801, whole genome shotgun sequence.
ACCESSION   DAORKP010000103 DAORKP010000000
VERSION     DAORKP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_8372_cov_8.061801"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1043
                     /locus_tag="PLN75_06720"
     CDS             69..1043
                     /locus_tag="PLN75_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRG03844.1"
                     /translation="MKTRNYFKLFLIISISVLSFTGCIKTEDEPKVDYYKVMTDYMAA
                     NKMDLPDVIASGVTTAAAVSANPAAFYIIDLRSATDFAAGHIQGAVNSTVKDVLTAAQ
                     NAGGKPILLVCYTGQNAAYANVCLRLSGYADSKIMKWGMSAWTTQLDKWSANVSNQAV
                     NHANWSTTNAIKTPVTFSLPTFTATAKVGADILKERVKVALDKGFVGSKVPDVLGTPA
                     NYFIVNYWTDADVNQFGHIKGAYRLNETLKLTDPVGFKNLNPTAVIVPYCWTGQTSAM
                     VSAYLTVLGYDAKGLMFGANNMIQDALLAAKKNSWNNEKPKTDNPVVK"
     gene            1144..2241
                     /locus_tag="PLN75_06725"
     CDS             1144..2241
                     /locus_tag="PLN75_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRG03845.1"
                     /translation="MKNIKYIIVALFATAFAFTAYSQGCVSGGGDVVGVHGFIQSEYN
                     YYMNGTDANGKSLDQNTFNINRARLGVVGSIPYDVDYYVMIEYSPFTPQNKVHLLDGY
                     VSYTRFSQWAKISVGQFKSPFSLEQNTACSGLYTVDRSEVVNQLAGPQRDLGFVVNGG
                     NDSTFVQYSVGMMNGSGMNVKDDNRFKDIVGRVVLSPLRSTGIDALKDLKLGGSFKYG
                     KINPTDNTQPLNEITRLGVELQYKLHGFLLQSEYILGTDKLHSASQVPVFGGCGGIVG
                     YETKSAGTYNKSGFWVIGSYMTPWNLEPTVKFDTYMADHAVTNSRTNRITVGLNYYIN
                     DYARLQMNYINAQESAGIVNDRFVVQLQAKF"
     gene            2272..3051
                     /locus_tag="PLN75_06730"
     CDS             2272..3051
                     /locus_tag="PLN75_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRG03846.1"
                     /translation="MKKIIFLFMSFVAFSLQAQLISVDELSKIIKEPNVVVIDARPAG
                     DYMKTHIDGAISVDAATLSTNSPVEGTLKSTGELASLLGKAGVSNKNKIVIYCKTGVN
                     AGLMYWVLDYLGAKDVSILDGQLDAWFAARKPITKNAKKLAATTFTPSVNAAVKVDKN
                     QVKAKMNSAVLVDTRKKEEYAAGKIGNALNIPSDAMLVKSKLKPAAALQQLFSEVPKN
                     KEVIVYCKTGTTASLAYFVLKSILKYPNVKVYEGAYLDWVK"
     gene            3227..3733
                     /locus_tag="PLN75_06735"
     CDS             3227..3733
                     /locus_tag="PLN75_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRG03847.1"
                     /translation="MKKYWILSLSSLLILLLGACSGNDHKSYKSVEEMVADAKASVTF
                     ISAQDFKKKVESGEKYYLIDCREAVEFDSVCIKGAINIPRTVLEAQVSDKAPKKRNTV
                     YIYCNNADKAILAASVLPEFKYAHVKVIDGGLDAVKAKFPELIELNPVRDGAKSKAPT
                     PASGGCGG"
     gene            3904..4317
                     /locus_tag="PLN75_06740"
     CDS             3904..4317
                     /locus_tag="PLN75_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03848.1"
                     /translation="MSKLVIGLSSGSIDKLVGAGVMMSGAAADDMDIEVYVLLTAGRA
                     FLKGNENLSDSLVEYPHLKDEMYARMKELGIDNWIEAWQEVKDLTNTKIFICSLAGKM
                     WGGDKLEDFNSLVDGFTGIFQYVQAAQAADLHISL"
     gene            complement(4498..5178)
                     /locus_tag="PLN75_06745"
     CDS             complement(4498..5178)
                     /locus_tag="PLN75_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRG03849.1"
                     /translation="MMEHNCTSCPFNRQAMNTLSQSSFDKLNANHAVVSFEKGSTIIK
                     QGMFSTNVAFLREGLVKLHLAGPYYEQIVRLVKAPTYLGLPTTFGNKINQYSATAISE
                     AEVCFIDIQIFRELLEKNHQFSYEIILELCRNELESFRRCANRTQKQSRGNIAAFLLE
                     LSGDIFQSDEFLLPLNQQEIGNLIDSTRESVSRILTEFSKDEIIRMDGKKIKILNRST
                     LELISKNG"
     gene            5317..5883
                     /locus_tag="PLN75_06750"
     CDS             5317..5883
                     /locus_tag="PLN75_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRG03850.1"
                     /translation="MEKNNEATILQLQSQMEALQKKVQRLENNRQDQLSMAIVSGDMD
                     KILAAMIISLAAAAMDTQVKLFFSFWALAALRDPKKNPKGKDFISKMFGMMLPKSRNN
                     LKLSNMNMAGMGPVMIKSLMKKKNVMSLDEMFRQAAELGIEITVCEMSMDLMGFKKEE
                     IIDYPHLRYAGATTFVADAGESAMQLFI"
     gene            6119..6370
                     /locus_tag="PLN75_06755"
     CDS             6119..6370
                     /locus_tag="PLN75_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRG03851.1"
                     /translation="MTTEELKNVAVAKSVDARGTACPGPLLEAKKAIGTIGVNDVMEV
                     LSADEGTKMDIPKWCNKQGHEYLGFVEENGYFKIYLKKQ"
     gene            6499..6912
                     /locus_tag="PLN75_06760"
     CDS             6499..6912
                     /locus_tag="PLN75_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRG03852.1"
                     /translation="MAIATKKTAKILVFSTEKISDPAIDMAGLMKLHYPPTVYTITVP
                     CSSGIKSKWILYAYEKGFDGVFIAADGSDCVYGESCTEKTGKLVTKTHELMKSQEIEP
                     ARLRMAAICSVCSESFVKQIRSFNDYLLKTLHPES"
     gene            6909..8213
                     /locus_tag="PLN75_06765"
     CDS             6909..8213
                     /locus_tag="PLN75_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRG03853.1"
                     /translation="MNQEKKNITYDALVIGAGIAGGEAALNLANTGFKVLLVDKDDSI
                     GGKMILLSKVFPTLDCAACITTPKVSEISRHPNITIFTGSKVDNIKRVSENEFTATIY
                     KKPRYVHVEDCTACQKCEEACPVSVSDQYQYGLVGRKAAYIPFSICNPKAAVIDIENC
                     TLCGACEKACPTDCIDFTMEEEQYDLHVRTVVLATGFNLFDPLKMPRYGFGTYKNVLT
                     SMQMERQIAPTRPFHTILRPSDGKMPDKIAYVLCTGSRDASVGNPICSQICCMYSIKQ
                     AQLLMGALPMADITIYYLNIRSFGKGFEEFYEQAKGMGVNFVKGKIGKITEKENGNLM
                     LRYEDINAGKLKVAEHDMVVLSVGALPNTEAKGMFADDLKLDKFEFVGQPDLMASPAQ
                     TSIEGVFVAGTASAPMDIPDAILSAGSASSEAISYLMKTQPH"
BASE COUNT         2674 a         1701 c         1839 g         2158 t
ORIGIN      
        1 tagtttatca gcttgctgaa aaattaggaa atcggaggag attcaaatat taaacaataa
       61 aaaatcttat gaaaacacgt aattatttca aactctttct gatcatttca atatcagttt
      121 taagttttac cggttgtatc aaaaccgaag acgagcccaa agtagattac tacaaagtaa
      181 tgaccgatta tatggctgcc aataaaatgg acttgcccga tgtaattgct agtggagtta
      241 ccactgctgc agccgtaagt gctaatcctg ctgcatttta tattattgac ttacgttctg
      301 caactgattt tgctgccgga catattcagg gtgcagttaa ttcaaccgtt aaagacgttc
      361 tcaccgctgc tcagaatgct ggtggtaaac cgattcttct ggtgtgctac acaggccaga
      421 atgcagctta cgctaacgtt tgcctgcgct tgagcggata cgcagacagc aaaataatga
      481 aatggggtat gagtgcctgg acaacacagc ttgacaaatg gagcgccaat gtatccaacc
      541 aggcagtaaa tcatgcaaac tggtcaacaa ctaacgccat caaaactccg gttacattct
      601 cacttccaac tttcacggca actgcaaaag taggtgctga cattctgaaa gaaagagtga
      661 aagtggcact tgataaagga tttgtgggct ccaaagttcc tgacgtgctt ggaactcccg
      721 ccaattattt tattgtaaat tattggacag atgctgatgt taatcagttt ggacatatta
      781 aaggtgctta tcgcctgaat gaaacattaa aattaactga ccctgtagga ttcaaaaacc
      841 ttaatcccac tgccgttatc gttccttatt gctggaccgg acaaacttca gccatggttt
      901 ctgcttatct taccgttctg ggttacgatg caaaaggact tatgttcggt gcaaataata
      961 tgatacagga cgctttgctt gcagcaaaga aaaacagctg gaacaatgaa aaaccaaaaa
     1021 ctgacaatcc ggtagttaaa taagtattga aagttttttt tgagagaaca gtcaatctct
     1081 cttggtttta gagagatttg gctgatttcc aattcattat tagttattct aaaaatcagt
     1141 aaaatgaaaa atataaaata tattattgtt gctttgtttg ccacagcatt tgcatttacg
     1201 gcatattcgc agggttgtgt gtccggaggc ggtgatgtag tgggtgttca cggtttcatt
     1261 cagtcggaat acaactatta tatgaatggt accgatgcca acggcaaaag cctggatcaa
     1321 aacacattca atatcaatcg tgcacgtttg ggcgtagttg gttccattcc ttacgacgta
     1381 gattattatg taatgattga atacagccct tttactccgc agaataaagt gcacttgctg
     1441 gatggatacg tgtcatacac acgcttcagc caatgggcaa aaatatccgt ggggcagttc
     1501 aaatctcctt tcagtttgga acaaaataca gcctgctcgg gactttacac cgttgaccgt
     1561 tcggaagtgg tgaatcaact tgccggtccg caacgcgatc tgggatttgt agttaacgga
     1621 ggaaatgatt caacatttgt tcaatatagc gtgggaatga tgaatggaag cggcatgaac
     1681 gtgaaagacg ataatcgttt caaagatatt gtaggtcgtg ttgttttaag tccattgaga
     1741 agtaccggta ttgatgcgct gaaagacctg aagctgggcg gcagtttcaa atacggaaaa
     1801 ataaatccga ccgacaacac ccagcctttg aatgaaatca cccgcttagg tgtagaatta
     1861 caatacaagc ttcatggttt tcttttgcag tccgaataca ttcttggaac agataaactt
     1921 cattctgctt cgcaggtacc cgtatttggc ggatgcggcg gtatcgtagg ttatgaaacc
     1981 aaaagtgcag gaacttataa taaaagcggt ttctgggtaa tcggttccta catgactccg
     2041 tggaacctgg agccaaccgt gaaatttgac acttacatgg cagaccacgc tgtaaccaac
     2101 agcagaacaa accgcatcac cgtaggtctg aattattata tcaacgatta tgcccgcctg
     2161 cagatgaatt atatcaatgc acaggaaagc gccggcattg tcaacgaccg ttttgtagta
     2221 caattacaag ctaaatttta aaaaattaaa aggatttaaa ttcagagaaa aatgaaaaaa
     2281 ataatatttc tattcatgtc atttgtggca ttcagccttc aggcacaact catctcggta
     2341 gatgaactca gtaaaataat caaagaaccg aatgtagtcg taatagatgc gcgtccggca
     2401 ggcgattata tgaaaacaca cattgacgga gctatcagcg tagatgctgc caccctttca
     2461 acaaacagcc ctgtggaagg aaccttaaaa agcactggcg aattagcatc acttttaggt
     2521 aaagcaggtg tttccaataa aaataaaatt gtgatttatt gtaaaacggg cgtaaatgcc
     2581 ggactcatgt actgggtgct cgattatctg ggagccaaag atgtaagcat actcgacgga
     2641 caactcgatg cctggtttgc agcccgtaaa ccaattacaa aaaacgctaa aaaactggct
     2701 gcaactacat tcacgccgag tgtaaacgct gcagtaaaag tggataaaaa tcaggtgaaa
     2761 gccaaaatga actcagccgt actggtcgat acccggaaaa aggaagaata cgcagccggc
     2821 aaaatcggga atgcactcaa catccccagt gatgcgatgt tagtcaaatc aaaactgaaa
     2881 ccagctgccg cacttcaaca gttattttcc gaagtaccta aaaataagga agtaattgtt
     2941 tactgcaaga ccggcactac tgcctcgttg gcatatttcg tactgaaatc cattctgaaa
     3001 tatccgaacg tgaaagtgta cgaaggtgca tacctggatt gggtgaaata agaagccccc
     3061 taaccctccc gaaacaagtt cggaacgggc tctaaagggg gaataactag agcgattctt
     3121 gactttcaaa gggtcaaata actatacttt ataacagaat attttaaatt tttgcactcc
     3181 aaaactcccc ctttaggggg ctggagggct aatatataat caaaaaatga aaaaatactg
     3241 gatattatca ttatcaagcc tgctgatttt attgctcgga gcttgttcgg gtaatgatca
     3301 taagtcttat aaatctgttg aagaaatggt tgcagatgct aaagcgtctg taaccttcat
     3361 cagcgctcag gattttaaga aaaaggttga aagcggcgaa aaatattatc tgatcgactg
     3421 ccgcgaagca gtagaattcg atagtgtctg tatcaaagga gcaatcaaca taccccgaac
     3481 tgtactcgaa gcgcaggtat ctgacaaagc tccgaaaaaa agaaatacag tatatatata
     3541 ttgtaataac gccgataaag caatacttgc agcatccgta ctgcctgaat tcaaatatgc
     3601 tcacgtaaaa gtgattgatg gcggtttgga tgcagtaaaa gcaaaattcc cggaattaat
     3661 cgaactgaat cccgtacgcg atggcgcaaa gtcaaaagca ccgacaccgg cctcaggcgg
     3721 atgtggcgga tagaagcgag ttaacagtaa aatagtaaaa tagtaaaatc gaatttgtcc
     3781 ccttttgcga ttcagaaagt cccaatttta ataaaaaaat ccttttgttt tcccccatgt
     3841 gggggaaatc tcgaagggaa aggtgtttgg ggatttaggg gacctaaaaa taaaaaaaaa
     3901 cttatgtcaa aattagtaat cggactatcg tccggaagta tagataaact ggtaggagcg
     3961 ggcgtaatga tgagcggagc tgctgccgat gatatggata tcgaagtgta tgtattgctc
     4021 acagccggta gagcattcct gaaaggcaac gaaaatctca gtgattcgct ggtggagtat
     4081 cctcacctga aagatgaaat gtatgccaga atgaaagaac tcggcatcga caactggata
     4141 gaagcctggc aggaagtgaa agacctcaca aatacgaaaa tatttatctg cagccttgcc
     4201 ggcaaaatgt ggggaggcga taaactggag gatttcaact cgctggtgga cggatttaca
     4261 ggcatttttc agtacgtgca ggcggcacag gcagctgatt tacatatcag cctttaggtt
     4321 aatgtgaaaa aggggatgta tcaatagtac acggatgaca ctgatttagc tgattttctc
     4381 agataaataa aaatataaat attgtaaaac gctgattaca acagcaataa aaatctgtgt
     4441 ttatccgtaa aatccgcgtt atacgtgtac ttttttattt tgagacaact cttttgatta
     4501 tccgttttta ctgatcagtt caagggtgct tctattcagg attttgattt ttttaccgtc
     4561 catccggatg atttcgtctt tgctgaactc cgtcagaatg cggcttacgc tttcgcgggt
     4621 tgaatcaatc agattgccga tttcctgctg gttgagcgga agcagaaatt catcggactg
     4681 aaaaatatca cccgatagtt cgagaagaaa agcggcaata ttaccgcggg attgcttctg
     4741 ggtacggttg gcgcaacgac gaaaactttc cagttcattg cggcaaagtt ctaaaataat
     4801 ctcgtaagag aactgatggt ttttttcgag caattcacga aaaatctgaa tgtcgataaa
     4861 acaaacctct gcctccgaaa tggcagtagc ggagtattga ttgattttat tgccgaatgt
     4921 agtaggaagt cccagataag tgggagcttt caccaggcgc acaatctgct cgtagtaagg
     4981 gcctgccaga tgcagtttca ccagtccttc gcgcagaaaa gccacgttgg ttgaaaacat
     5041 tccctgtttg ataatcgtgc ttcctttttc aaagctgact actgcgtgat tcgcattcaa
     5101 cttatcaaaa ctgctctggc taagcgtatt catagcctga cgattaaagg gacaagaggt
     5161 gcaattgtgt tccatcatgt tgcaaatata gtaaatgtga tttaaatcac aaaatactgt
     5221 aagaataaac actgaataat tatgaaaagt gattgtaaaa taatatacat ttgctgaaaa
     5281 atttcttcaa tataaaacat ccaaaaatat tatctaatgg aaaagaataa cgaagcaaca
     5341 attctgcagt tgcagtcaca gatggaagct ttgcagaaaa aagttcaacg gctggaaaac
     5401 aatcgtcagg atcaactctc catggcaatt gtatctggcg atatggataa aattctggca
     5461 gccatgatta tttcgctcgc agcggcagct atggatacac aagtaaagct ctttttctct
     5521 ttctgggcgc tggcagcctt acgcgatccg aagaaaaatc cgaaaggcaa agactttatt
     5581 tcaaaaatgt ttggaatgat gctgccgaaa agcagaaaca acctgaaact atcgaacatg
     5641 aacatggcag gcatgggtcc ggtgatgatt aagtcactga tgaaaaagaa aaacgtgatg
     5701 tcgctggatg aaatgtttcg tcaggcagcc gaactgggca tcgaaatcac cgtttgcgaa
     5761 atgtccatgg acctgatggg cttcaaaaaa gaggaaatca tagattatcc acacctgcgt
     5821 tatgcaggag ctaccacatt tgtggccgat gcaggcgaaa gcgctatgca gctgtttata
     5881 taaaacagat cactgccccc caaccctccc gaaataaatt cgggacaggc tctgaagggg
     5941 gaaatggagc attttttaag caaattttac aaaaaaaatt tctaaataca tataacattg
     6001 acctttgcac tttaaattcc ccaactactg ttttccactt cctttctcca actggagaaa
     6061 gtccccgaag ggggatagag gttgggggct agggggccgg tcttaaacat ttacaattat
     6121 gacaacagaa gaattaaaaa acgtagcagt agcaaaatca gtagatgcac gcggtacagc
     6181 atgtccgggt ccgctgctcg aagcaaaaaa agcaatcggc accatcggtg tgaatgatgt
     6241 aatggaagta ctttcggccg acgaaggaac aaagatggat ataccaaaat ggtgtaacaa
     6301 acagggacat gaatatctcg gtttcgtgga agaaaacgga tatttcaaaa tttatttgaa
     6361 aaaacaataa gatgaaaaat gaactcccaa ccggttggag aaaaatcaaa tctctgcccg
     6421 gagggagttt tttatccttt tttatttcac cgaatttttt tttctgaatt ttcataactc
     6481 agaaactcaa tcaatattat ggctattgca actaaaaaaa ctgcaaaaat actggttttc
     6541 tctaccgaaa agatttcaga cccggctatc gatatggccg gattgatgaa actgcattat
     6601 cctccaacag tatataccat tacagtgcct tgttccagcg gaataaaatc caagtggatt
     6661 ttgtatgctt acgaaaaagg atttgacggt gtatttatcg ctgccgacgg gagcgactgt
     6721 gtgtatggcg aaagctgtac agaaaaaacc ggtaaattag tgacaaaaac acatgaactg
     6781 atgaaatcac aggaaatcga accggcacga ctgcgtatgg cagctatctg ctccgtttgc
     6841 agcgaatctt ttgtgaagca aatcagaagt ttcaacgatt atttattaaa aactttacac
     6901 ccagaatcgt gaaccaggag aagaaaaaca taacgtatga cgcgttagtc atcggagcag
     6961 ggattgccgg aggcgaagcc gccctcaacc ttgccaatac aggcttcaaa gtactgctcg
     7021 tggataagga cgacagcatt ggaggaaaaa tgatcctgct gagcaaagta tttcccaccc
     7081 tcgattgtgc cgcctgtatc accacaccga aagtttcgga gatatccaga catccgaata
     7141 tcaccatctt cacaggaagc aaggttgata atattaaaag agtaagcgaa aacgaattta
     7201 ctgcaaccat ctacaaaaaa ccgcgatatg tacacgtgga agactgcacg gcttgtcaga
     7261 aatgcgagga agcctgcccg gtaagtgtca gcgaccagta tcagtacgga ctggtgggac
     7321 gtaaggctgc ctacattccg ttcagcatct gtaatcccaa agccgcggtc attgatatag
     7381 aaaactgcac gctctgcggg gcctgcgaaa aagcctgccc gaccgattgc atcgatttta
     7441 ccatggaaga ggaacagtac gatttacacg tgcgcaccgt agtgcttgcc acgggcttca
     7501 acctctttga tccgttgaag atgccgcgtt acggatttgg aacctacaaa aacgtactta
     7561 cctccatgca aatggaacgc cagatagctc ccacgcgtcc gttccacacc atcttgcgtc
     7621 ccagcgacgg aaaaatgccc gataaaatag cctacgtgct gtgtacaggt tcgcgcgatg
     7681 cttcggtggg caatcccatc tgctcgcaaa tatgctgcat gtattccatc aagcaggcac
     7741 agctcctgat gggcgcactg cctatggctg atatcaccat ctattacctc aatatccgct
     7801 ccttcggaaa aggttttgaa gaattctacg aacaagccaa aggaatggga gtgaatttcg
     7861 ttaaagggaa aataggtaaa atcaccgaaa aggaaaacgg caacctgatg cttcgttatg
     7921 aggatatcaa tgccggaaaa ctcaaagtgg cagaacacga tatggtagtg ctttcggtgg
     7981 gcgctttgcc caataccgaa gcaaaaggta tgtttgccga cgatctgaag ctggacaaat
     8041 tcgaattcgt gggacaaccc gacctgatgg cctcacctgc ccagacaagc atcgaaggtg
     8101 tgtttgtagc cggaaccgcg tcagcaccga tggacatccc cgatgctatc ctgtccgcag
     8161 gctcggcatc gtccgaagcc atcagttatt taatgaaaac acagccccac taaatctctc
     8221 caaaggggag acttattaaa agggtataac aataaaaaag tctatacata ttataaacta
     8281 actcattttc cccctttggg ggaattaaaa ggggctttta tggaaaaaag aataggagta
     8341 tacatttgtc attgcggtgg aaacatttca ga
//
LOCUS       DAORKP010000104         8193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1355_length_8193_cov_8.834112, whole genome shotgun sequence.
ACCESSION   DAORKP010000104 DAORKP010000000
VERSION     DAORKP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_8193_cov_8.834112"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3052)
                     /locus_tag="PLN75_06770"
     CDS             complement(<1..3052)
                     /locus_tag="PLN75_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG03854.1"
                     /translation="MKILKIELQNINSLKCESPIVIDFEHSRFEDVGLFAITGPTGAG
                     KTTLLDAITIALYRKVPRFEKPGFTGKLEDAVSYGAATALARVTLEVRNERYEAHWDI
                     RTATNNGRQLNKPVETVRLKNLSTGKILAETKTGCDEKIVEITRLNYEQFLRSVLLAQ
                     GEFAAFLSARNAEKGLLLQQIAGDEIYRKIGETLKNRISVERKQLEQIKSRINTDDLL
                     SREVTEQLEAEKVRLAKNSEELNDELKKTEELLKWYEQRNKLQLQKQKIENERNTLEL
                     EKTQHAADFYLLERHEWAEPLQPTLTEVLRLEKEIEIKKNRIAEIEKELEELETKLTQ
                     AQREVKNCKLRTDEHELSARQWQPKLEKVKELDTQISSHLKNIEEKRTRKEEINRTLL
                     QLKKEEEAKNKELAEQKLLLEQYEKYLQNNPNIPAFEKQLGRWNSQLTQRQSNLQRLT
                     ELKKDIAESRKNITENTSGIEKTGKSLKSEQEKLDLLVGDIKILENFLKKNNVEELFR
                     ENNKLTERKTFIAELITFSENHCELTKKQQEDTKIIAGLRQKTDELSGQLQSIQKDIE
                     TATLLVTDAETLYEKDRYIVSLEAERKKLKKGEPCALCGSTEHPLVEKYAETEISDSK
                     KRLEERKTQLVILQNQQIRLDNELTEHRTAHRLAETQFNKTNETLEKVTRQFTEKKPD
                     FGINEKTAMTAALNETEKKQQETSALMTEIQNCLKRKDEKSVLTKSIDEMITGLQKEQ
                     IRLSTANQAIEKHMEECIKTGTEIKSRNDELEQILTKELSESGFQLPETDRTASFIRE
                     LENKVTTYNDTAKKRSDAENKMSQINIELNNILRIRFEKSKEAETTEAEISDIQKALS
                     VDTESRNAILAAEISTDNKRLQLQNAIDLSKQEEENAAKQLDELQVVKTSLDGEKKSL
                     RNQHSEQTAELCERETALGDMIGKSNFADRNELLASLLTDEQKTRILKIRNGINERTI
                     SLSSLEKENSLEMENHEKELMPALSENETGVRIHSLNGQKEELQK"
     gene            complement(3101..4312)
                     /locus_tag="PLN75_06775"
     CDS             complement(3101..4312)
                     /locus_tag="PLN75_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRG03855.1"
                     /translation="MKILHTSDWHLGHRLLDQSQLEEQSLFLEWLPDYIEANSIDVLL
                     ISGDIFDVGVPSANAQKLYYDFLIRLHHSCCREVIITGGNHDAPSTLNAPKELLNALS
                     IRVVGKATEPISDEIFKLTRDNEELIVAAIPYLRDQDIRRAVAAETADEIVNRYKTAL
                     ISHFGEAAESCERMKSANTTVIGMGHLFAVGGRTSESEQSIYVGNLGDIGAEDFPTVF
                     DYVALGHLHRAQRVGGREHIRYSGSPYMLSFSETNQEKKIILIETGKPLIITETTIPV
                     FRKILQLEGSVESCKAQLEKLDEENNSLTPWVEVILNNEKEAATGYREINKFAEHLKS
                     EVLKITVKDEKKIDRLKSLVENAQHITGFKPLDVFKLRCEEQNLDLNERPEMLDAFNE
                     ILQTALNPENP"
     gene            complement(4391..5200)
                     /locus_tag="PLN75_06780"
     CDS             complement(4391..5200)
                     /locus_tag="PLN75_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03856.1"
                     /translation="MTDKNSNKKNQIRQENEELKKKMEEEFGAASQWSSPDLSPEMEN
                     DFLKHIMEFENAWKNTKKTTVYEFLGKPVFRRVENLQPEEISAELERMYTLMEKNQIS
                     LDTICEVPDDELYRFITEELFNEEIDDVRIEGMMSCYTYEEFHPNHEYDLNRYANDFI
                     QSYLDKTSDDYLHEISSDASKSDWHKHFRDAFSRFDVQKFEIESLNYNLETAKAEVGF
                     ECDIQAKVEGESDLFRFVGKGKFGFVHQWDFWYVDSVEFPKPYNESSAVRN"
     gene            5434..5874
                     /locus_tag="PLN75_06785"
     CDS             5434..5874
                     /locus_tag="PLN75_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03857.1"
                     /translation="MDRHEPKSVEISELFITFANPIQKRIMLIFDNILFATFIPQILM
                     FLGFVACVSASYLYPPPEPEKETMSEYRPATIVADNVSHSKTFLFTDYDFFANPAVTQ
                     ADSSDPNIPHISRSVYPLVTTSLSADGSDFLLFTRPPPVFYKLA"
     gene            5985..>8193
                     /locus_tag="PLN75_06790"
     CDS             5985..>8193
                     /locus_tag="PLN75_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03858.1"
                     /translation="MKKILFSVLALACIGIPAYALENDSLKTIRLGQVDVYSPKETNL
                     QNAPVSSTTLGADKINQSQIFSIRDISGVVPNFFIPDYGSCMSASPYVRGVGSRNSGQ
                     SIALYVDNVPYPEKSAFDFELYDLAQIEVLRGPQGTLYGRNSIGGIVNMYTLSPLNYQ
                     GVRASVTAGNYGMMQAKASYYTKLGEKTGFSAGGYYGKHDGFYKNDYTSKNLDAEKTA
                     GARVKFAWTPTQALKLEYATNFDYVDQGAFPYGLYNAESRTVANPNFNDASSYRRKTW
                     NNSLSSKYIFDNMTLTVSLAHQYFDDQMNLDQDFSPASIFTLQQNQKQQMLNGEAVLK
                     SAMKSNYSWLFGLNGFGQRLDTSAPVSFREDGIRTILQPVFTKIGQASGMTMTIKNPT
                     LDIPGTYDTKRGGGAMFHQSTFRNILVDGLSLTVGARLDIEKVALDYNSSSYLDIEAK
                     RGQIVMGTQRATAELIGTADTTFVEFLPKAALKYEWDHMNFIYGSVSRGYKTGGFNIQ
                     MISDLVRDKLQNTRPGAPAQETDVKHAVMYQPEHSWNYEAGIQNAFLDRKLRTSLTLF
                     YMDIKGLQLTEFVASGAGRMLTNAGTAVSKGLEVSADMKISKELNAGLNYGYAHASFN
                     NYTQVETLNGQPAEVDYSGKFVPYAPQQTANIYLNYVKTFENSFISQFYGNISYNGIG
                     KIYWQESNETAQDFYSLVDVKLGVKRDAFGLELWGKNLLNTTYNAFYFESFGNK"
BASE COUNT         1867 a         1838 c         1802 g         2686 t
ORIGIN      
        1 tcttttgcag ttcttctttt tgcccgttga gtgaatgtat ccgtacaccg gtttcatttt
       61 ccgaaagggc cggcatcagc tctttttcgt ggttttccat ttccaggctg ttttcctttt
      121 cgagtgagga aagcgaaata gttcgctcgt tgatgccgtt tcgtattttc agaatgcgtg
      181 ttttctgttc gtcggtgagg agtgaggcaa gcagttcatt ccggtcggca aaattacttt
      241 ttccaatcat gtccccaagt gcggtttccc gttcgcagag ttcggcggtc tgttccgaat
      301 gttgattcct taaactcttt ttttcgccat caagacttgt tttaaccacc tgcaattcat
      361 ccagctgttt cgctgcgttt tcttcttcct gttttgataa atcgatggca ttctgcagct
      421 gcaaccgttt attatcggtc gaaatttcgg cagctaaaat ggcgttgcgg ctttcggtat
      481 caacactaag ggctttttga atatcggaaa tttccgcttc ggtcgtttcg gcctcttttg
      541 atttttcaaa cctgattctg agaatattat tgagttcgat gttgatttga ctcattttgt
      601 tttcagcatc tgatctcttt ttcgcagtgt cgttgtaggt tgttacttta ttttcaagtt
      661 cccgtataaa tgacgctgtg cggtctgttt cgggcagttg aaatccggat tcgctgagct
      721 cttttgtcag gatttgctcc agttcgtcgt tcctgctttt aatttcagtt cccgttttga
      781 tacattcctc catgtgtttt tcaatagcct gattagcggt tgaaagccgt atttgttctt
      841 tctgaaggcc tgtaatcatt tcatcgatgc tttttgtcag aaccgacttt tcgtctttgc
      901 gtttcagaca gttctgtatt tcggtcatca gagcagatgt ttcctgctgt tttttttcgg
      961 tttcattcaa agcggctgtc atggccgttt tttcattaat tccgaaatcc ggtttttttt
     1021 cggtgaactg tcttgttacc ttttcgagtg tttcgttggt tttgttgaac tgagtttctg
     1081 ccagccgatg ggctgtgcgg tgttcggtta attcattgtc aagcctgatt tgctggtttt
     1141 gcaggatgac gagttgcgtt ttgcgttctt cgagcctttt tttgctgtcc gaaatttctg
     1201 tttcggcata tttttccacc agcggatgtt cagtagaacc acacagggca cacggctcgc
     1261 cttttttcag cttttttctt tcggcctcga ggctcactat gtagcggtct ttctcgtaca
     1321 gtgtttcagc atcggttacc agcagagtgg ctgtttcgat gtctttctga atggactgaa
     1381 gctgtccgga cagttcatct gttttctgac gaagacctgc gattattttt gtgtcttcct
     1441 gctgcttttt ggtaagttcg cagtggtttt ccgaaaaggt aatcagttcg gcaatgaaag
     1501 tcttccgttc tgtgagcttg ttgttttccc tgaacagttc ttccacgttg ttttttttta
     1561 gaaaattctc cagtattttt atatccccta ccagcaaatc cagtttttcc tgctctgatt
     1621 tcagcgattt tccggttttt tctattccgg atgtgttttc agttatgttt ttcctcgatt
     1681 cggcgatgtc ttttttaagt tctgtaagtc tttggaggtt gctttgacgc tgtgtgagct
     1741 gtgaattcca gcgtccgagt tgcttttcaa aggcaggaat gttcggattg ttttgcaggt
     1801 acttctcgta ttgctccagc aacagctttt gctccgcaag ttccttattc ttagcttcct
     1861 cctctttctt aagttggagt aaagtccggt ttatttcttc tttccgggtt cttttttctt
     1921 caatattctt caggtgtgag ctaatctgtg tgtcgagttc tttaactttt tccagtttgg
     1981 gctgccattg cctggccgat agttcatgct catcagtgcg cagtttgcag tttttcactt
     2041 ctctttgagc ctgtgtcaac ttcgtttcga gttcctccag ctccttttca atttctgcta
     2101 tccggttttt tttgatttca atctcctttt ccaacctgag tacttcggtc agcgtgggtt
     2161 gcagtggttc tgcccactcg tgtctttcca gtagataaaa atctgctgca tgttgtgttt
     2221 tttccagctc cagggtgttt ctttcgttct caatcttctg tttttgcagt tgaagcttgt
     2281 ttcgctgttc gtaccatttc agcagttctt ccgttttttt tagctcgtcg ttaagttctt
     2341 ccgagttttt agccagccgg actttttctg cttccagttg ttcggttact tctcggctga
     2401 gcaaatcgtc ggtgttgatc cggcttttga tttgttcgag ctgttttctt tcgaccgaaa
     2461 tccggttctt gagtgtttct ccaattttgc ggtatatttc gtcgccggca atctgctgca
     2521 gcagcaatcc tttttcggcg tttctggccg agagaaatgc cgcgaattca ccctgagcga
     2581 gtaatacgga gcgcagaaac tgctcgtagt tgagccgggt gatttccaca attttttcgt
     2641 cgcatcctgt tttggtttca gcaagtattt ttcctgtgct cagatttttc aacctgacgg
     2701 tttccacggg tttattcagc tgccggccgt tgtttgttgc cgtgcggatg tcccagtgtg
     2761 cctcgtagcg ctcgttacgg acttccaggg ttactcttgc cagcgcggtg gcagctccgt
     2821 agctcactgc atcttcgagc tttccggtga atcctggttt ttcaaaccgg ggtacctttc
     2881 gataaagggc tatggtaatg gcatcgagga gcgtagtctt gcccgctccg gtaggtccgg
     2941 taatggcaaa gagacccaca tcctcaaaac gggaatgctc aaagtcaatc actatggggc
     3001 tttcgcattt cagcgagttg atattttgca gttctatctt tagtatcttc atgtgttagt
     3061 ggttggttta cagttaataa tcccttactt cgtacgggaa tcagggattt tccggattca
     3121 gtgcggtttg cagtatttcg ttgaaggcat cgagcatttc cggtctttca ttcagatcca
     3181 ggttctgttc ttcgcacctg agtttgaaaa catccagcgg cttaaaacct gtgatgtgct
     3241 gcgcattttc caccagactt ttcagcctgt cgattttttt ttcgtccttt acagtaattt
     3301 tgagaacttc ggattttaaa tgttcagcaa atttgtttat ttcccgataa ccggtggctg
     3361 cctctttttc gttgttcaga ataacttcca cccagggtgt cagcgaattg ttttcctcgt
     3421 cgagtttttc aagctgtgct ttacagcttt ccaccgaacc ctccagctga agaattttac
     3481 gaaacacggg aatagttgtt tccgtaataa ttaacggttt gccggtttcg atgaggatga
     3541 tttttttctc ctggtttgtt tcgctgaaac taagcatgta cggcgaaccc gaataccgga
     3601 tgtgctcacg gccgcccacg cgctgagcac ggtgaagatg tcccagcgcc acataatcaa
     3661 atacagtggg gaaatcttcg gctccgatat cacccagatt acccacgtag atgctttgct
     3721 cgctttctga agtgcgcccg cctacagcaa acagatgccc cataccaatc acggtggtat
     3781 ttgcagattt cattcgttca catgattcgg ctgcttcacc gaaatggctt atcagggcag
     3841 ttttgtagcg gttgacgatt tcatcggccg tttcggctgc cacggcacgt cgtatatcct
     3901 gatcgcgcag gtaggggatt gccgccacaa taagttcctc attatcacgc gtcagtttga
     3961 aaatctcatc gctaatcggc tcggtggctt taccaaccac acggattgaa agtgcattca
     4021 gcaactcttt gggtgcattc agtgtgcttg gcgcatcgtg attgcctccg gtgatgatta
     4081 cttcgcggca acaactgtgg tgaagccgta tcagaaaatc atagtagagt ttctgtgcgt
     4141 tggccgaagg aactcccaca tcgaaaatgt cgcccgaaat gagcagaaca tcgatagagt
     4201 tagcttcaat gtagtccggc agccattcga gaaagagcga ctgttcctcc agttgcgact
     4261 ggtcgagcag gcggtgaccc aaatgccagt cggaagtatg tagtattttc atggagattt
     4321 aaatgatggt tttttttgtt ttaaatatca tcaggatggc aagtgttgtt cagaaatttt
     4381 tatatttcat tcaatttctt acggcagatg attcgttgta aggttttgga aattctaccg
     4441 aatccacata ccagaaatcc cattgatgaa caaaaccgaa ttttcctttt ccgacaaacc
     4501 ggaataaatc cgactcacct tctacttttg cctgaatgtc gcattcaaaa ccgacctctg
     4561 cttttgcggt ctcaaggtta taattcaggc tctcaatctc aaacttctgt acatcaaaac
     4621 ggctgaatgc atctctgaaa tgtttgtgcc agtcgctttt tgaagcatcg gagctgattt
     4681 cgtgcaggta gtcgtccgaa gttttatcca ggtaagattg tatgaaatca tttgcgtagc
     4741 ggttgagatc atattcgtgg ttggggtgaa attcttcgta cgtgtaacag gacatcattc
     4801 cctcaatacg tacgtcgtca atttcttcgt tgaataattc ttcagtaata aaacggtaaa
     4861 gttcatcatc cggaacctcg caaatagtat ccagactaat ctgatttttt tccatcaacg
     4921 tgtacatacg ctccagttcc gcactgattt cttccggttg caggttctca accctacgga
     4981 agaccggttt gccaagaaat tcgtaaacgg tggttttctt tgtatttttc caggcgtttt
     5041 caaattccat aatgtgtttt aagaaatcgt tttccatttc aggcgacaag tccgggctgc
     5101 tccattgact tgctgccccg aattcttctt ccatcttctt ttttaattct tcgttttcct
     5161 gccggatttg attttttttg tttgaatttt tgtcggtcat agtggtttct gtaataaggt
     5221 ttatatatag ttataattca tggctttcag tgagttctct tcgatatttt cagaatgata
     5281 cgcccttgta ttcgtttact caacaaatat acagatttat ttttatcccc gctttctctc
     5341 cggaataaaa tccaacttca tgagcagtac tccgttttat ttttatgaat ttaaccggtt
     5401 atttcttacg taaatgttgc tttatccaat cttttggata gacatgaacc gaaatcggtg
     5461 gaaatatcag aactatttat tacttttgct aacccaattc agaaacggat tatgctgatt
     5521 ttcgacaaca tactttttgc tacgttcata ccccagatac tgatgtttct gggttttgtt
     5581 gcctgtgttt cggcatctta tctgtacccg ccgcctgaac cggaaaagga aaccatgtcc
     5641 gaatacagac cggctacaat tgttgctgat aatgtgagtc acagcaaaac ttttctgttt
     5701 acggactatg acttttttgc aaatcctgca gttactcagg ctgacagcag cgatccaaac
     5761 attccgcata tcagccgatc cgtttatccg ttagtcacaa cttcactctc tgctgacggg
     5821 tctgattttc tgctttttac gcgccctcct cctgtttttt acaaattagc ataaaaaatt
     5881 cttcaatcag ttttctggtt tttggtatga tgcgggtttt tgcaaattta cttttgctta
     5941 aacagctctt ttataactat ttgtaaaaaa taaattattc aaaaatgaaa aaaatattat
     6001 ttagtgtttt ggctttagca tgcattggta ttccggctta tgcacttgaa aacgacagtt
     6061 taaagacaat ccgtcttgga caggtggacg tgtacagtcc taaggaaacc aatctgcaaa
     6121 acgctcccgt ttcatcgaca acgctcggtg ccgacaaaat caatcagtcg caaatttttt
     6181 ccatcaggga cataagcggc gtggtaccca actttttcat ccccgattac ggttcctgca
     6241 tgtcggccag tccgtatgtt cgcggtgtgg gttcgcgtaa cagcggacag tccattgctt
     6301 tgtatgtgga taatgtgcca taccccgaga agtcggcttt tgacttcgaa ctgtatgacc
     6361 tggctcagat tgaagtgctg cgcggtccgc agggtacact ttacggccga aacagcatag
     6421 gaggtattgt taatatgtac accctgtcgc cgctcaacta tcagggtgtg cgggcatcgg
     6481 tcactgccgg aaactacggt atgatgcagg ctaaagcatc gtattatacc aaattgggtg
     6541 aaaaaaccgg attttcggcc ggcggttatt acggaaagca cgacggattt tataaaaatg
     6601 attacacctc gaaaaatttg gatgccgaaa aaacggccgg tgcgcgtgtg aaatttgcct
     6661 ggacacccac acaggcactg aaactggagt acgccaccaa cttcgattat gttgatcagg
     6721 gtgcgttccc ttacggactg tacaatgccg aaagccggac ggtagccaat ccgaatttca
     6781 acgacgcgag cagttaccga cggaaaacct ggaataacag cctgtcatcc aaatatattt
     6841 ttgataatat gacattgacg gtttcgctcg cccatcagta tttcgatgat cagatgaatc
     6901 tcgatcagga tttttcaccg gcatccatat tcaccctgca gcaaaaccaa aaacagcaga
     6961 tgctgaacgg agaagcggta ctgaaatcgg ctatgaaaag caattatagc tggttattcg
     7021 gtctgaatgg tttcggacaa cggctcgata cttcggctcc ggtgagtttc cgggaagatg
     7081 gaattcgtac cattcttcag ccggtgttta cgaaaatcgg acaggcatcc ggcatgacaa
     7141 tgacgatcaa aaatcctacg cttgatatcc cgggtacgta cgataccaaa cggggcggag
     7201 gagccatgtt tcaccaatcc acattccgga atatattggt cgatggactt tcgcttaccg
     7261 ttggcgcacg tctggacatt gaaaaggtgg cgctggacta caactcgagc tcctacctgg
     7321 acatcgaagc caaaagaggc cagatagtaa tgggaacaca gcgtgcaaca gccgaactga
     7381 tcggcactgc cgatactaca tttgtggaat ttctgccaaa agcagcgctg aaatacgagt
     7441 gggaccatat gaactttatt tatgggagtg tatcacgcgg ctataaaacc ggcggattca
     7501 atattcagat gatatccgac ctggtcagag acaaattgca aaacacccgt ccgggagctc
     7561 ctgcacagga aacagatgtg aaacatgccg tcatgtacca acccgagcat agctggaact
     7621 acgaagcagg cattcaaaat gcttttctgg atagaaaact ccgcacttcg ctcacacttt
     7681 tctacatgga tatcaaagga cttcagctca ccgaattcgt agccagcgga gccggcagaa
     7741 tgcttaccaa tgccggaacg gctgtgagca aagggctgga ggtttcggct gatatgaaaa
     7801 tcagcaagga gctgaacgcc ggactgaact atggatatgc tcacgcatca ttcaataatt
     7861 acacacaggt agaaacgcta aacggacagc cggctgaagt ggattactcc ggcaagtttg
     7921 tgccttatgc accccaacaa acagccaaca tctacctgaa ttacgtgaag acattcgaaa
     7981 actcttttat ttcacagttt tatggaaata tttcatacaa tggtatcggt aaaatctact
     8041 ggcaggaatc caatgaaact gctcaggatt tttacagcct ggtggatgta aaactgggag
     8101 tcaaaaggga tgcatttggt ttggaattat ggggcaagaa cctgttgaat acaacttata
     8161 atgcatttta tttcgaatca ttcggcaaca aat
//
LOCUS       DAORKP010000105         8154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1371_length_8154_cov_9.700827, whole genome shotgun sequence.
ACCESSION   DAORKP010000105 DAORKP010000000
VERSION     DAORKP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1371_length_8154_cov_9.700827"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLN75_06795"
     CDS             complement(<1..847)
                     /locus_tag="PLN75_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRG03859.1"
                     /translation="MKLYRLNKSLVRALIMTFLAFTFISGLIAQTSRPKQLRNEILQS
                     KTKEDKRTTSTSRKNRLGNPSRVGATVLKPVNPAAKNRGSSMVQLERSNTLLYNQDLY
                     PGIQILLGNVVFRHDNALLFCDSAYFYEQANSFDAFSNVRIVQADTLTVYGDFLNYNG
                     NTKLARIWDNVKMVNRNTVLTTNVLYYDRNLNLAFYDTGGVIEDGKNTLTSMWGQYSP
                     ATKVALFKDQVKMTNPDMNMTTDTLKYNTSSSVADIVGNSLIVYKAETDIYSQRGWYD
                     TKNDRM"
     gene            complement(925..2298)
                     /locus_tag="PLN75_06800"
     CDS             complement(925..2298)
                     /locus_tag="PLN75_06800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRG03860.1"
                     /translation="MRKLSLLLLALTLLTGGLKAQQNKKNIIDEVIWIVGDEAILKSD
                     VETMILDAEINRRPIEGNPYCIFPEQIAIEKLYLHQAAIDSITVNESYINSISDRELE
                     YYINNAGSAQKLEEYMRRPISQLRDEIRTRNRTRSMAQQMQAKLVEKIEATPAEVRRY
                     FSNIPADSLPTIPEQVELQVLSLLPPIPPAEINRVKEQLREFTERANKNPGDFSLLAR
                     LYSDDVESAKKGGELGFAGRGIYDPDFASVAFNMQDPNKVSRIVETQFGFHIIQFIER
                     RGDKVNVRHILMRPKATSDVKTQGINKLDSLANLIRENKITFEQTVMAYSQDKNTRMN
                     AGLMMQQDKYTGSITSKYEYQDLPPEIARAAYAMNVGEVSKAFTMIDQTTGNEVIAVV
                     KMKSKIPTHKANVKEDYQMLKSFLEEKKKSEFLQEWVAKKQKETYISIDPEYRDCNFT
                     YSGWLNK"
     gene            complement(2374..3222)
                     /locus_tag="PLN75_06805"
     CDS             complement(2374..3222)
                     /locus_tag="PLN75_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03861.1"
                     /translation="MKKLLFFILLSVIVSCSEKKSDNGRTPILEVEGKFLYKDETDKI
                     IPPNSTVIDSADIADRYIKKWATEVLMYENAKRNIDNLDEINKLADEYRQSLIIHAYE
                     QALVAERLDPKISEKEAKNFYEEFKTHLTLEDNLIKGMLLIVPVGAPQMEAVKEWVRK
                     GDTKSLEKIEKYSLQNAISYDYFMNKWTPFTEITKKAPFVTENTSGFVLNTRFAEVSD
                     SINSYLLHISKAIAAGDTEPYDYAKERITTTLLNRKKSDFIITFEKNVYNDALKKGNI
                     KFYKKK"
     gene            complement(3320..5260)
                     /locus_tag="PLN75_06810"
     CDS             complement(3320..5260)
                     /locus_tag="PLN75_06810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRG03862.1"
                     /translation="MKKVFVSLLVLVLASNALMAQQSDPVLMKINGKNIPLSEFEYIY
                     NKNNSNNVVDKKSLDEYVDLFVNFKLKVEEAITQGLDTTQSFKSELGMYRNQLAEQYL
                     NDSKALDKLVEEAYSRKTEEVEVRHILFRIPETGTSADTLQVYNKALAIWKRAQKEDF
                     ESLAREVSEDPSVAQNGGYVGWISTMRTPYSFENMAYSTPVGKVSKPVRTFIGYHIIK
                     VEGKRQSQGEVKVSHILLMNDRQHPENNVSVKQRTDSIYQRILAGDDFGELAVKLSQD
                     PGSARQNGELPWFGSGQMIPEFENAAFALRNKGDVSQPVESQFGWHIIKLIDKKPVGS
                     FDDLKASLEGQIKQNERNKMTGDSFAENLKKEYNFTQEPNYLADFLKLSDNYAPSDSM
                     FAVEARKMNLPMFSFGGQQFTQKDFADYLLSGARTFRGVRSDFIQDNYKEFVNSRLVA
                     YEQTQLDRKYPDFHNLMQEYHDGILLFEVMNNEVWEKATKDTEGLKRYFEANKAEYAW
                     AKPHYKGHIIHAKDKATMKAAQKIVKRALPDSISTYLNKRLNDSIQYVKVEKGLWVEG
                     ENPVIDFRIFKKGTYTPSKEYPFYFVAGKMLKTLPEEYSDVRGIVTANYQDYLEKEWI
                     AGLRKKYPVTIDEKVLKTVKKN"
     gene            complement(5446..6924)
                     /gene="guaB"
                     /locus_tag="PLN75_06815"
     CDS             complement(5446..6924)
                     /gene="guaB"
                     /locus_tag="PLN75_06815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRG03863.1"
                     /translation="MSFIADKIEFEGFTFDDVLLVPAYSEVLPRNVSLTTRFSRNIEL
                     KIPVVSAAMDTVTEAKMAIAIAREGGIGVIHKNMTIAEQARQVSMVKRAENGMISNPV
                     TIRQGATVADALSLMAEYKIGGIPVVDDDGYLMGIVTNRDLRFQRDLSKLIDEVMTKE
                     NLVTTSRTTNLEEAAEILQQYKIEKLPVVDENYKLIGLLTYKDITKAKDKPMACKDSR
                     GRLRVAAGVGITSDTFERIEALVEAGVDAIVIDTAHGHSKGVIDALKKVKSRFTEIDV
                     IVGNIATAEAALALVESGADAVKVGIGPGSICTTRIIAGVGVPQLSAIYAVAHALQDT
                     GVPVIADGGIRYSGDIVKALAAGADTIMAGSLFAGVEESPGETIIFNGRKFKSYRGMG
                     SLEAMDKGSKDRYFQDMEDDIKKLVPEGISARVPFKGSLYEVVYQMIGGLRAGMGYCG
                     ANNIGELHQAKFTRITSAGVVENHPHDVTITSEAPNYSRGDN"
     gene            complement(7093..7809)
                     /locus_tag="PLN75_06820"
     CDS             complement(7093..7809)
                     /locus_tag="PLN75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRG03864.1"
                     /translation="MKKAIVVGASSGIGRELARLLVKNDYKVGITARRENLLNELYAE
                     NPDSYVVGAFDLSEIDVIVPNLNRITEELGGLDLVVISAGLSGQTLDFDFKVEFESLQ
                     INVMAFTCICNWAFRIFREQNHGHLAGISSVAGARGWRNSKVYNAAKAYQINYLEGLR
                     NHAAYLKLPVTVTTIIPGYVETRMKGNNYVFWVAPVEVAARQIYNSIRKKRKIIYTYR
                     RWRFIYFLFKIFPNKLIEIS"
BASE COUNT         1940 a         1734 c         1783 g         2697 t
ORIGIN      
        1 tcatgcggtc atttttggtg tcgtaccagc ctcgctgact gtaaatgtcg gtttcagctt
       61 tatatacgat gagcgaattt cctactatat ctgccaccga actgcttgta ttgtatttca
      121 gggtgtcggt ggtcatattc atgtccggat tggtcatttt cacctgatcc ttgaacagtg
      181 ctacttttgt agcgggcgag tactgccccc acatggacgt aagtgtgttt ttaccatctt
      241 ctatcacacc accagtatcg taaaatgcca gattgaggtt tcggtcgtag taaagcacgt
      301 tggtagtcag cactgtattt cggtttacca ttttcacgtt gtcccagatg cgtgccagtt
      361 tggtgtttcc attgtagtta agaaaatcgc cgtaaacagt aagcgtgtca gcctgcacta
      421 tccgaacgtt gctgaaagca tcgaatgaat tggcttgttc gtaaaaataa gcactgtcgc
      481 agaataacaa tgcattgtcg tgtctgaata ccacatttcc taataaaatc tggataccgg
      541 gatacaaatc ctggttgtag agcagtgtat tgcttcgctc cagctgcacc atggatgacc
      601 ctctgttttt agcagccgga ttaacaggtt taagtacggt agcgcctact ctcgacggat
      661 tgcccagccg gtttttccgt gaggtggaag tggttctttt atcttctttg gttttggatt
      721 ggagtatttc gttgcgcaat tgtttaggcc tgctggtttg agctatcagg cctgatataa
      781 aagtaaaggc aagaaaagtc attatcaatg ccctgaccag cgatttattt agtctgtaaa
      841 gtttcatttg cagtgaagag attgattgag catagttcct ttatatcaca ggttcatcac
      901 ttaccattct ctttttcggg ttttctattt gttcagccag ccgctgtaag tgaagttaca
      961 gtcgcggtat tccggatcaa tgcttatgta ggtctctttc tgctttttgg ccacccattc
     1021 ttgcagaaat tcgctttttt tcttttcttc gaggaaagat ttcagcattt ggtagtcttc
     1081 cttcacgttg gctttgtggg tgggtatttt tgatttcatt tttactaccg ctattacttc
     1141 gttgccggta gtctggtcaa tcatggtgaa agcctttgac acttcaccca cattcattgc
     1201 atacgctgcc cgggctattt cgggtggtaa gtcctggtat tcgtacttcg aagtgattga
     1261 tccggtgtac ttatcctgct gcatcatgag tccggcgttc attcgggtgt ttttatcctg
     1321 cgagtaagcc attacggttt gctcaaatgt gattttattt tcacggatga ggttggccag
     1381 tgagtccagc ttgtttattc cctgtgtttt gacatcagaa gtggctttag ggcgcataag
     1441 tatatggcgt acgttcactt tgtcgccgcg acgttcgatg aactgaatga tatgaaaacc
     1501 gaactgtgtt tcaactattc tggatacttt gttcggatcc tgcatattga aagctacgga
     1561 tgcgaaatca gggtcgtaaa ttcctcttcc ggcaaatcct aattcgcccc cttttttggc
     1621 gctttccaca tcatccgaat aaagtcttgc aagcaggctg aagtctccgg gatttttatt
     1681 ggcccgttcg gtgaattcgc gcagttgctc tttcacgcgg ttgatttctg ccggtggaat
     1741 gggaggaagc aaagataaaa cctgcagttc aacctgttca gggatggtag gaaggctgtc
     1801 ggccggaatg ttgctgaagt agcggcgtac ttcggccgga gtagcttcga ttttttcaac
     1861 gagtttggcc tgcatctgct gagccatgct tcgtgtgcgg tttcgtgtgc gtatttcgtc
     1921 tctaagttgt gagatgggac ggcgcatata ctcttcaagt ttctgagccg aaccggcatt
     1981 attgatgtag tattccagct ccctgtcgga aatgctattg atgtagcttt cgttcactgt
     2041 tatgctatcg atggcagcct gatgcaggta tagtttttcg atggctatct gttccggaaa
     2101 aatgcaatag ggattccctt caatgggtct ccggttgatt tcggcatcca gtatcatggt
     2161 ttcaacgtcc gatttcagta tcgcttcatc accaactatc catattactt cgtcgatgat
     2221 gttttttttg ttttgttgtg ctttaagtcc gcctgtgagc agggtaagcg ccagaaggag
     2281 caaagataat tttcgcatat tcgttatgtt cattaccggt tttttgccgg atccgattct
     2341 tatgtttttt taaaaatatt gtttgtgaat tgattatttc tttttataaa actttatatt
     2401 tccttttttc aaggcatcgt tgtacacgtt tttttcaaat gtgataataa aatccgattt
     2461 ttttctgttc agcagggtcg ttgtgattct ttcttttgca tagtcataag gttcggtatc
     2521 gcctgctgca atggctttgg agatatgcag taaataagaa tttatactgt cagaaacttc
     2581 ggcaaatcgt gtgtttagta caaaacctga agtattttca gttacaaaag gagctttttt
     2641 ggttatttca gtgaatggag tccatttgtt cataaaataa tcgtaactga tggcattttg
     2701 caaactgtat ttctcaattt tctccagcga tttagtatca ccttttctta cccattcttt
     2761 tacggcttcc atttgaggtg ctcccacagg cactataagc aacattcctt ttatgaggtt
     2821 atcttcaagt gtgagatgtg tcttgaattc ttcatagaag tttttggctt ccttttccga
     2881 aattttcgga tccagtcgtt cggctacgag cgcctgttcg tatgcatgta tgatgagcga
     2941 ttgtctgtat tcatctgcca gtttgttgat ttcatccagg ttgtcgatgt tgcgttttgc
     3001 attttcgtac atgagcactt cggtagccca tttttttatg tatcggtcgg caatatctgc
     3061 gctgtcaatt acggtcgaat tgggtggtat aatcttatct gtttcgtctt tgtacagaaa
     3121 ttttccttcc acctccagta tgggtgtccg cccgttatcc gacttctttt cggagcagga
     3181 tacgatgact gaaaggagta tgaagaacaa caattttttc attcgaaagg gcagaagatt
     3241 aaccacatac caatgggata cagcaatgcc agaataagaa ttcaaaggta gaacttttat
     3301 tctggcattc gttaaaaaat tagtttttct taaccgtttt cagtactttt tcgtcaatgg
     3361 tcaccgggta tttttttcgc aatccggcta tccattcttt ttcgaggtaa tcctgatagt
     3421 tggcagtaac tataccccgt acatcggagt attcttcggg tagtgttttt agcattttgc
     3481 cggctacaaa gtaaaacggg tattctttgg aaggtgtgta tgtgcctttt ttgaaaattc
     3541 taaagtcaat aaccggattt tctccttcta cccagaggcc tttttctact ttcacgtatt
     3601 gaatactgtc gttcagtctt ttattcaggt aggtgctgat ggaatccggc agggctcttt
     3661 tgactatttt ttgggcagct ttcatcgttg ccttgtcttt tgcatggatt atgtgacctt
     3721 tgtagtgcgg ttttgcccat gcatattctg ccttattggc ttcaaaatac cgtttcagac
     3781 cttccgtatc tttggtggct ttttcccata cttcgttgtt cattacttcg aagagtaaaa
     3841 ttccgtcgtg gtattcctgc ataagattat gaaaatcagg atatttacgg tcgagctgtg
     3901 tctgttcgta agcaacaagc cggctgttga caaattcttt atagttgtcc tgaatgaaat
     3961 cgcttctcac tccgcggaat gttctggcac ctgaaagcag gtagtcggca aagtcttttt
     4021 gagtaaattg ctgaccgccg aacgaaaaca tgggcaggtt catttttcgg gcttctacgg
     4081 caaacatgct gtccgatgga gcatagttgt ccgatagttt cagaaaatca gccagataat
     4141 taggttcttg tgtgaaattg tattcttttt tcagattttc ggcaaaagag tcacctgtca
     4201 ttttattgcg ctcgttttgc tttatttgtc cttccagaga ggctttcaga tcatcgaaac
     4261 ttcccaccgg ctttttgtca atgagtttga taatgtgcca tccaaactgc gactcgacgg
     4321 gttgtgaaac atcgccttta ttccggagtg caaaagcggc attctcaaat tcaggaatca
     4381 tttgtccgct tccgaaccag ggtaattcgc cattttgacg tgctgagccc ggatcctgtg
     4441 aaagttttac ggccaactca ccgaaatcgt cgccggcaag tatgcgctga tagatggaat
     4501 ctgttctttg ttttactgaa acgttgtttt cagggtgctg acggtcgttc atgagcagaa
     4561 tgtgcgatac tttcacttcg ccctgcgact gacgtttgcc ttccactttg atgatgtgat
     4621 aaccgatgaa cgtgcgaacc ggtttcgata cctttccgac aggagtgctg taagccatgt
     4681 tttcgaaact atagggagtg cgcatggtcg aaatccagcc cacatatccg ccgttttggg
     4741 ctacgctcgg atcttcggac acttcacgag ccaggctttc aaaatcttct ttttgagcac
     4801 gtttccagat tgcaagtgct ttgttgtaaa cttgcaatgt atcggcagaa gtacctgttt
     4861 cgggtatccg gaataagata tgccttacct ctacctcttc ggttttgcgg ctgtaggctt
     4921 cttctaccag tttatccagg gctttgctgt cgttcaggta ttgttcagcc agttgattgc
     4981 ggtacatacc cagttctgat ttgaaagatt gagtggtatc caatccctga gtaatggctt
     5041 cttcaacttt caacttgaaa ttcacaaaca aatccacgta ttcatccagc gattttttat
     5101 caactacgtt gtttgaatta ttcttgttgt atatatattc aaattccgac aggggaatgt
     5161 ttttcccatt aattttcatc aataccggat ctgactgctg tgccatcagt gcatttgaag
     5221 ccagaaccaa aaccaaaagg gaaacaaaaa cttttttcat ctgtttgaac tatttttttt
     5281 atgaacttaa attgttatta tctttattcg caatgttaaa cgttttgaat cagaaaaaat
     5341 tgtttgatgt ttgaaaatgt gtctgcaatt cagaattcag aacacggagc ttgaaacctg
     5401 gaatctggaa ccctgcctgc ctcgggcagg tcagaactca gaactctaat tatctccccg
     5461 gctgtaatta ggtgcttcac ttgttatggt cacatcgtgc ggatgatttt cgactactcc
     5521 ggcgctggta attcgggtga atttggcctg atgaagctct ccgatgttat tcgcaccaca
     5581 gtaacccatt ccggcgcgaa gtcctccaat catctgataa acaacttcgt acaaggaacc
     5641 tttgaaggga acgcgggctg aaattccttc cggaaccagt ttcttgatgt cgtcttccat
     5701 gtcctgaaaa taacggtcct tggagccttt gtccatggct tccagtgagc ccattccccg
     5761 ataggatttg aatttacgtc cgttgaaaat gatagtttcg ccaggtgatt cctccactcc
     5821 ggcaaacaat gatccggcca tgatggtgtc ggctccggca gccagtgctt tcactatatc
     5881 acccgaatag cggatgcctc cgtcagctat caccggtact ccggtgtcct gcagtgcatg
     5941 tgctacggca tagatggccg aaagttgcgg aacgcccaca ccggcaatga tgcgtgtggt
     6001 acagattgaa cccggtccga ttcccacttt cacggcatca gctcccgatt ctacaagagc
     6061 cagggcagct tcggcagtgg caatgttacc tacaatcacg tcgatttcgg taaaccggga
     6121 tttaactttt ttgagagcgt caattacccc tttcgagtgg ccgtgagccg tatcaatcac
     6181 aatggcatcc acgccggctt ctaccagtgc ttctatgcgt tcgaatgtgt cggaggtgat
     6241 tccaactccg gctgccacgc gtaagcggcc ccgactgtcc ttgcaggcca tgggtttgtc
     6301 tttggctttg gttatgtcct tgtaggtgag cagaccgatg agtttgtaat tctcatccac
     6361 tacaggtaat ttctctattt tatactgctg tagaatttcg gctgcttcct ccaggttggt
     6421 ggtgcgcgat gttgttacca aattttcttt ggtcattacc tcatcgataa gcttactcag
     6481 gtcgcgctga aagcgcaaat cgcggttggt gactattccc atcaggtaac catcgtcgtc
     6541 caccacgggt attccgccga ttttgtattc cgccatcaac gaaagggcat cggctaccgt
     6601 agctccctgc cggatggtta ccggattaga aatcattcca ttttcggcac gtttcaccat
     6661 gcttacctga cgggcttgtt cggctatggt catattcttg tgtataactc caatgccgcc
     6721 ttcacgggct attgcaatag ccattttagc ttcggtgact gtatccatgg cagccgaaac
     6781 tacagggatt ttcaattcaa tgtttctgga aaaacgggtg gtgagactca cgttgcgggg
     6841 aagtacttca gagtaagccg gaacgagcaa cacatcgtcg aaagtaaagc cttcaaactc
     6901 tattttatct gcaataaaag acatagtatg agtgttttag cgttgttaat acctatgaaa
     6961 aatattgcct gcaaatgtaa tcattttttg tgggtaatgg attattttat ttgtgaaaaa
     7021 acagtaaaat gagtttcctc ttcaggtggc ttgactggct ctatgcaatt acactgctga
     7081 tagcagtgct tgtcagctta tttctatcag tttgttcgga aagattttga aaagaaaata
     7141 gatgaatctc cagcgccggt atgtgtaaat tatttttctt tttttacgga tggaattgta
     7201 tatctgtctg gctgccacct ccacgggtgc tacccagaat acataattat tgcctttcat
     7261 gcgggtttcc acatatccag gtattattgt ggttaccgtg acgggcaatt taaggtaagc
     7321 tgcgtggttt ctgagtcctt cgaggtaatt tatctggtag gctttggcgg cattgtaaac
     7381 tttgctgttg cgccagcctc tggctcctgc caccgacgat atgccggcca gatgtccatg
     7441 gttttgctct ctgaaaatac ggaatgccca gttgcatata caggtaaatg ccattacatt
     7501 gatctgaagt gattcaaatt ccaccttaaa gtcaaaatcc aaagtctgac ccgataaacc
     7561 tgcactgata acaaccaggt ccagtcctcc aagctcttcg gtgatccggt tgagattggg
     7621 gacaatcaca tcaatttcac tcaggtcgaa agctcccact acgtatgaat cgggattttc
     7681 ggcgtaaagt tcattgagca gattttctct tcgggctgtt ataccaacct tgtagtcgtt
     7741 tttaaccagt aagcgagcca gttctctgcc aattccggag gaggcaccta caacaattgc
     7801 tttcttcata acagatatgt tcacataaat tacttcaaaa taactgaaaa tcaacaacat
     7861 ctattcagtg aaatcactaa acgcatgaaa ccggtaaatc aatacaaatg taagtaaatt
     7921 aattgagctt gtatcttaaa atttaactta ttgaaaaaac tatttgagtt gaaaaagtaa
     7981 atattctaca aacacgcatc ctgatgtgat gatgatctca tcatatgtat cttaatatct
     8041 cgagtgaatt aactcagtct tgtgtaatgg atatttagct gatttttttt ttagaaaagc
     8101 aggagtgaaa atatatattg aaattaaatg taaagaattt gttatttgca ttct
//
LOCUS       DAORKP010000106         8033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1409_length_8033_cov_6.674981, whole genome shotgun sequence.
ACCESSION   DAORKP010000106 DAORKP010000000
VERSION     DAORKP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_8033_cov_6.674981"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1931)
                     /gene="ilvB"
                     /locus_tag="PLN75_06825"
     CDS             complement(255..1931)
                     /gene="ilvB"
                     /locus_tag="PLN75_06825"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRG03865.1"
                     /translation="MNGAHAFMQALIHEGVDTIFGYPGGAIMPVFDALYDYSDKVNHI
                     LTRHEQGATHAAQGYARVSGKVGVSLVTSGPAATNAITGIADALLDSTPLIVITGQVG
                     TSLLGTDAFQEIDVVGITQPITKWSYQIRYAKDIAWAVARAFYIARSGRPGPVVLDFT
                     KSAQNEKVNFDYKPCNFIRSYIPVPDIRMDQIEAAADLINKAEKPYALVGQGVILANA
                     EKALKEFLEKADIPAASTLLGLSAMSTDFRLNKGFLGMHGNVAPNMKTNECDVLIAIG
                     MRFDDRVTGDLNTYAKQAKIIHLDIDQSEIGKNVTVDAPVLGSVADSLPALTKKLKPA
                     SHTEWVESFREHDNREHEAVIRKEVYRTEGELTMGEVIHKITEATGNQAIVVTDVGQN
                     QMMAARYFKYSKTRSMVTSGGLGTMGFGIPAAIGAKMGAPERTVCMFAGDGGFQMTIQ
                     ELGTIMQEQIGVKMIILDNRFLGMVRQWQELFFDERYSFTKMHNPDFVAIAKAYNIDG
                     KLVTKREDLDDAISDMLKDDKPYLLVVEVEQKGMVYPMVPAGTCVTNIIM"
     gene            complement(2084..4009)
                     /gene="ilvD"
                     /locus_tag="PLN75_06830"
     CDS             complement(2084..4009)
                     /gene="ilvD"
                     /locus_tag="PLN75_06830"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRG03866.1"
                     /translation="MAGARALWKANGMTTEQMGKPIIAIVNSFTQFVPGHTHLHEVGQ
                     MVKAEIEKLGCFAAEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHKADAM
                     ICISNCDKITPGMLMATMRLNIPTIFVSGGPMEAGILDGKQLDLVDAMVLAADDNVSD
                     EQVKRVENAACPTCGSCSGMFTANSMNCLNEAIGLALPGNGTIVATHVNRKQLFVDAA
                     AIIVENAWKYYRDGDDSVLPRNIATRDAFINAMTLDVAMGGSTNTVLHILAIAQEAEV
                     EFTMDDIDEISRKTPCVCKVAPNSNKFHIQDVNRAGGILGIMGELSPPKSPHGGTFEE
                     PPSPKSPHGGTFEEPPSPKSPHGGTFEEPPSSKSPQKGLLNLGVKRVDGLTLGEAIEK
                     YSITKENVHPDAGRIYGSAPANRFNLVMGSQESYYNTLDTDRMEGCIRSLENAYTKDG
                     GLAILKGNIAQDGCVIKTAGVDESIWHFEGKAKVYTSQDTACSGILKGEVVAGDVVVI
                     IHEGPKGGPGMQEMLYPTSYIKARQLGKACALITDGRFSGGTSGLSIGHISPEAAAGG
                     NVGLLQDGDVIEIDIPNRKINVKLSDEELQLRRESEMARGKDAFKPKDRNRTVSKALR
                     AYASMVSSADKGAVREI"
     gene            complement(4683..5339)
                     /locus_tag="PLN75_06835"
     CDS             complement(4683..5339)
                     /locus_tag="PLN75_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRG03867.1"
                     /translation="METKHRVHNLILLDESGSMTSIKNTIIQGFNELVQTVKGIALQY
                     PEQEHLISFFTFNGMGIKTLHFCEPVEKLSEIDERKYQPDAMTPLYDAMGFSFGKLRK
                     ELESITDFNVLVTILTDGEENSSREYSGEAIKRLVEELSQQRWTFTYIGADHDVEKFA
                     VSISISNTMKFSKNEEDMKQMFAKEQSARAIYSRKIRMKEETGLFFYDEDDDKKDDKK
                     "
     gene            complement(5481..6395)
                     /locus_tag="PLN75_06840"
     CDS             complement(5481..6395)
                     /locus_tag="PLN75_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRG03868.1"
                     /translation="MDQPKVERLLRIMKMLTGNTSLTIEEIAGQLSISPRSVYRYIDT
                     FRDAGFVIKKKNNCFRLDKSSPYFKDISSLIHFSREEAYILKSAIESIDENNVIKQNL
                     KKKLYSVYDYNVLAETIINTKNGNNVKRLVEAMENKRTVILHNYASSHGNDIRDRRVE
                     AYAFTTNYVQVWCYCPEEECNKLFRVSRIGRVEVLPEKWKSIRKHKPGFIDIFRMNSS
                     RTFHIKLQLGLRAKNLLVEEFPLAEPLLTALPQNQWLLETNVCSAEGVGRFIMGLPDD
                     IEIIDSPEITQYIMSLVDKMKNKYNVTS"
     gene            complement(6854..7951)
                     /locus_tag="PLN75_06845"
     CDS             complement(6854..7951)
                     /locus_tag="PLN75_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03869.1"
                     /translation="MKSTLLNSVIALLFVVIGVSCDSEPAGVSLKESLDKGVKELNAA
                     VDKIKTSADFQLLSQNDESAATGMAKAPAFSEWYFMPNDTVMITLDKVAGIYDYSWQR
                     VKTNNPYYRFFTKTGESEYMIVRLPVQKVRHPAAMFWHQKADTALTNNFEAKVTDYYV
                     FRNRKAGNEYRLTSDFSIDNAQIGSLTELSSRNKVNGYNASSVYTLASGYTVSRTENS
                     GDTAVSAYAISEGDKILYEEKMTSVRLTDKKIRYREKTYSLTVGNVKIVRNLKPESMD
                     SAQVYLNGVLQTNSKVEIIVIQPDSLNQCLTHATRDLKITLDDGTSTTLKTLMNETID
                     GIQQIFRSVQQARFTTDIIDRIAWNIYSRKK"
BASE COUNT         1864 a         1785 c         1839 g         2545 t
ORIGIN      
        1 caaaccaacc gtattttctg aaaaaacggt gatggtatat aattttgttt ccataatgtc
       61 agccccctaa cccccaaaag gggaatttcc cggattttac aagaggggtg ggaaattaaa
      121 atttgttttt tagaatactt attgtttttg aaatgctaag cgtttgcacc attattcccc
      181 cttaagagcc tatcccaaac aatcccgatt tcataacggg aaacttggtt tgggagggtt
      241 aggggcgtgt ctttttacat aattatatta gtaacacagg ttccggcagg caccatcgga
      301 tacaccatgc ctttttgttc cacttccact accagcaggt atggtttgtc gtctttcagc
      361 atgtcgctga tggcatcgtc aagatcttcg cgtttcgtca ctaattttcc gtctatgttg
      421 tatgctttcg caatggccac aaaatccgga ttgtgcattt tggtgaacga atagcgttcg
      481 tcgaagaaaa gttcctgcca ctggcggacc atacccagaa accggttgtc gagtataatc
      541 attttcacac caatttgctc ctgcatgatg gttccgagtt cctgaatggt catctggaat
      601 cctccatcgc ctgcaaacat gcatacagta cgctcgggag ctcccatttt agcaccgata
      661 gctgccggaa tcccgaatcc catggtgcct aaaccgcccg aagtgaccat acttcgtgtt
      721 tttgaatatt tgaaatatcg ggctgccatc atctgatttt gtcccacatc ggtcactacg
      781 attgcctgat ttccggtggc ttcagttatt ttgtgaatta cttctcccat tgtaagttct
      841 ccttcggtac gatacacttc tttccggatt accgcctcgt gttccctgtt gtcatgttcc
      901 ctgaaacttt ccacccattc tgtgtggctg gctggtttta gttttttggt tagcgcaggc
      961 aggctgtcgg ctaccgagcc cagtactggt gcatccacgg tcacattttt gcctatttcc
     1021 gattgatcaa tgtccagatg aatgattttg gcttgtttgg cgtaagtatt caaatcgcct
     1081 gttacccgat cgtcgaatcg cattccgatg gcaataagca catcacattc gttggttttc
     1141 atgtttgggg ctacgtttcc gtgcattccc agaaatcctt tgttcagccg gaaatctgtt
     1201 gacatggccg aaagtccgag cagggtggaa gctgccggta tatcggcttt ctccagaaat
     1261 tctttaagtg ctttttcggc gttagccaga attacgccct gaccgacaag tgcataaggt
     1321 ttttcggcct tgttgattaa gtcggccgca gcttctatct gatccatccg tatatccgga
     1381 acaggtatgt agctacgaat gaaattgcac ggtttgtagt caaaatttac tttctcgttt
     1441 tgagcacttt ttgtaaagtc gagtactacc gggccgggcc ttccgcttcg ggcaatgtag
     1501 aaagcacgcg ctacagccca ggcgatgtct ttggcgtacc ttatctggta cgaccatttc
     1561 gtaataggct gcgtaatgcc taccacatct atttcctgaa aagcatcggt accgagcagc
     1621 gaagtgccca cctgaccggt gatgactatc agcggagtgc tgtcaagcag tgcatcggct
     1681 atgcctgtga tggcgttggt ggctgccgga cccgaagtga ccaggcttac gcctactttt
     1741 cccgaaacgc gtgcatagcc ctgagcggcg tgggtagctc cctgctcgtg acgggtgaga
     1801 atatgattga ctttatccga ataatcgtaa agagcatcga atacgggcat gattgcgccg
     1861 ccgggatacc cgaaaatggt gtcaactcct tcgtgaatga gtgcctgcat gaatgcatgt
     1921 gcaccgttca tgatttgttc tccggctccc tgagtggtgg gattggagtg tagttcctga
     1981 gtttttgtgt ttttgttcat actaatttat ttcgatgtta tcagtaatct gttttttttt
     2041 gagaaaatgc agccttttct gcaaatattg aagtcgggag aatttaaatt tctctcaccg
     2101 ctcccttatc ggccgaactt accatgcttg cgtaggctct cagtgcttta gaaacggtcc
     2161 ggtttctgtc cttgggtttg aatgcatctt ttccgcgtgc catttcggat tcgcggcgga
     2221 gttgaagttc ctcgtcggat aattttacgt ttattttccg gttcggtatg tcaatctcta
     2281 ttacatcacc gtcctgtagc aagcctacgt tgcctccggc ggctgcttcg ggcgaaatat
     2341 gtccgatgga taagcccgat gttccgcccg agaagcggcc atcggttatc agtgcgcagg
     2401 ctttaccgag ttgcctggcc ttgatgtatg aagtagggta gagcatttcc tgcataccgg
     2461 gtcctccttt gggcccttcg tggattatca ctaccacatc gccggctaca acctcacctt
     2521 tcagaatgcc actgcaggca gtatcctgtg aggtatatac ctttgctttc ccttcgaagt
     2581 gccagatgct ttcgtccaca ccggcagttt tgattacgca gccgtcctgt gctatgtttc
     2641 ctttcagaat ggcgagtccg ccgtctttgg tgtaggcatt ttccagtgaa cggatacaac
     2701 cttccatgcg gtcggtatcc agcgtattgt agtaagattc ctgtgatccc atcaccaggt
     2761 tgaatcggtt cgcaggcgcg ctgccgtata ttcgcccggc atcgggatgt acattttctt
     2821 tggtaatgga atatttttcg atagcttcac ccagcgtcag tccatccacc cgtttcacac
     2881 cgaggtttaa aagccccttt tgaggtgatt tagaagatgg gggttcttca aaagtccccc
     2941 catgggggga tttaggggat gggggttctt caaaagtccc cccatggggg gatttagggg
     3001 atgggggttc ttcaaaagtc cccccatggg gggatttagg ggggcttaac tctcccatta
     3061 tccccaatat tcctccagct ctgttcacgt cctgaatatg gaatttgttc gaattgggtg
     3121 ccactttgca tacacagggc gttttacgtg aaatttcatc gatatcgtcc atggtaaatt
     3181 ccacttcagc ttcctgtgct atggctaaaa tatgcaatac ggtattggtg gaaccgccca
     3241 ttgccacatc cagcgtcatg gcgttaatga atgcatcgcg ggtggcaatg ttccttggaa
     3301 gaacactgtc gtcgccgtcg cggtaatatt tccaggcatt ttctacaatt atcgcggctg
     3361 catccacaaa gagttgtttg cggttgacgt gtgttgccac gatggttccg ttaccgggca
     3421 atgccagccc gattgcttcg ttcaggcaat tcatggaatt ggctgtaaac atgcccgagc
     3481 atgatccgca agtgggacaa gctgcattct ccaccctttt aacttgttcg tcggagacat
     3541 tgtcgtcggc agccagtacc attgcgtcca ccagatccag ctgtttacca tccagaatgc
     3601 cggcttccat cggacctccc gacacaaaaa ttgtcgggat gttcagtcgc atggtagcca
     3661 taagcatgcc cggggttatc ttatcgcagt tgctgatgca gatcatcgca tctgctttgt
     3721 gtgcattcac catgtattcc acactgtcgg ctatgaggtc acgcgaggga agtgagtaga
     3781 gcatcccgtc gtggcccata gcgatgccgt cgtccacggc aatggtgttg aattctgcag
     3841 caaaacatcc cagcttttct atttccgctt ttaccatctg acccacttcg tgcaggtggg
     3901 tatgtccggg aacgaactgg gtgaatgagt ttacgatggc aataatcggt ttgcccatct
     3961 gttcggtggt cattccgttg gctttccaga gtgcgcgggc tcctgccata cggcgtccct
     4021 gagtggatgt ggcggaacga agttgacctc caaacctccc cgaagcagcc cctccaaacc
     4081 tccccgaagg ggaggcttca ggattgtggg gtttattatt attttgctga ttgttcatta
     4141 ttctgagttt ttctatgtaa gaaattacct tgttctgtta ttgcttttaa gaaatttctc
     4201 cttctggtca ggttgtaact ggcgaaggat tcaatccttt tttaattaaa aaaacctttc
     4261 tcggattgtg tgagaaaggt tttattgctt tatataaaca tttgtaaaca ctacaaccgt
     4321 tctcacctct gatttttgac caaaagagta atgacgagga cgagtagaat gttgacgctt
     4381 tgagtagtca tttgttgttt ttttctgaat tgtttgcaaa gataaggcaa gatttttaat
     4441 gtgccaatag tttaacacta aaaaatcaaa ataattgcaa aattgtaagt tttattagtc
     4501 tttttgtgag ttttgatagt atcgggtgaa aaattattcg ttaaactgtt ttttttgttg
     4561 tcactgccaa cctgaaggca ttgcagttat tccatcaaaa aagccacctg actcagtggc
     4621 ttttctgatt gtaaacaaac gaaatgataa ccggattaag gtttatcgtt ttagttgatt
     4681 attcattttt tgtcgtcttt tttatcgtca tcttcatcgt agaaaaaaag gccggtttct
     4741 tctttcatcc ggattttcct gctatagatg gctctggccg attgttcctt tgcaaacatc
     4801 tgtttcatat cttcctcgtt tttgctgaat ttcatggtgt tgctgatgga gatggagacg
     4861 gcaaatttct ccacatcgtg gtcggctccg atgtaggtaa atgtccatcg ctgctgcgat
     4921 aattcttcta ccagcctttt gatggcttcg cctgaatact cgcgtgagga attttcttcg
     4981 ccgtcggtaa ggatggttac cagtacgttg aagtcggtaa ttgattcgag ttcttttctg
     5041 agcttgccaa aactgaatcc catggcatcg tacagcgggg tcattgcatc cggctgatat
     5101 tttctttcat ctatttcgct gagtttttct accggctcgc agaagtgcaa ggttttgata
     5161 cccatcccgt tgaatgtgaa gaatgaaatc aggtgttcct gttcggggta ttgaagggct
     5221 atacctttca cagtttgtac cagctcgttg aatccctgaa taatggtgtt tttgattgac
     5281 gtcatcgaac cgctttcgtc aagcagaatg aggttgtgaa cccggtgttt tgtttccatc
     5341 tttgtgttgg ttaaaaggtt aaatgtgtgt tttgaaccat gaaaccacaa agatacaaca
     5401 caggtttgac agcttttgac gcaaaaagac tgatttactt tttaactaat atttaacatt
     5461 ttattgttaa cttgcagttc ttacgaggtt acattgtatt tatttttcat cttatcgacc
     5521 agactcatta tgtattgagt gatttcgggc gaatctatga tttcaatatc gtccggaaga
     5581 cccatgatga atcgtcccac accttcggct gagcacacat tggtttcgag cagccactgg
     5641 ttttggggga gtgcggtgag caggggttcg gccagtggaa attcctccac gagcagattt
     5701 ttggcacgta gtccgagttg aagttttata tggaaagtcc ggctgctgtt catcctgaaa
     5761 atatcgatga agccgggttt gtgtttccgg atggatttcc atttttcggg cagcacttca
     5821 acccttccaa ttcgcgatac cctgaaaagc ttgttgcatt cctcttcggg gcagtagcac
     5881 catacctgca cgtagttggt ggtgaatgcg taggcttcca cgcggcggtc gcgtatgtcg
     5941 ttgccgtgcg acgaagcgta attgtgcaga atgaccgtgc gtttgttctc catggcctcc
     6001 accagccgtt taacgttgtt tccgtttttg gtgttgatga tggtttcggc cagtacgttg
     6061 tagtcgtaca cggagtagag tttcttcttc agattttgtt tgataacgtt gttctcgtcg
     6121 atactctcga tggccgattt cagaatgtag gcttcctcgc ggctgaagtg aatcaggctg
     6181 ctgatgtctt tgaagtaagg cgatgacttg tcgagcctga agcagttgtt ttttttcttg
     6241 attacaaaac cggcatcgcg aaaagtgtcg atgtagcgat acactgaacg gggtgagata
     6301 gacagctgac cggcaatttc ttcaatggta agcgacgtgt tgcccgtgag catcttcatg
     6361 atgcgaagca ggcgttctac tttgggttga tccatggtat ctgattgtat gattgaagtg
     6421 aataattgat ttcaacttac gctactaatc aaacctgttg atgaatgtca gaaaagcagc
     6481 aaaaaaaact cgtttttcag cgaaatcatt cagccggaca tattcacgag aaatgctgcc
     6541 agacaaagat acaataaact ttgagagaaa aaacttttta ttttattcct ttttgctgaa
     6601 tatgatgatt ttttcctgaa tgattcctac agataatcat taatgccggt gcaatgcagt
     6661 ggtctgtaaa cttctttttt tttatgaaat aactttagag tttacctcag gcagttgatc
     6721 aaccgtgaga aggtgggata atgacttttt accataaaaa atttctgcat gctgccctga
     6781 atacattaac catacgtttt cggacaaaaa aaggccggaa aaaatccggc cccgtaacag
     6841 cacttaaata cagttatttt tttcgcgaat agatattcca ggctatccgg tcgatgatat
     6901 cggtggtaaa tctggcttgc tgtacgctcc tgaagatttg ttggattcca tcgattgttt
     6961 cattcatgag tgttttcagc gtagtggatg tgccgtcatc taaagtgatt ttcagatcgc
     7021 gcgtagcatg tgtaaggcac tgattgagcg aatcgggttg gataacaatg atttcaacct
     7081 tcgaattggt ctgcagtact ccattcagat acacctgagc gctgtccatg ctttcaggtt
     7141 tcagattacg tactatcttt acgttgccca ctgtaagtga gtaggtcttt tctctgtatc
     7201 ttattttttt atccgtaagg cgtaccgaag tcattttttc ttcatacaaa atcttatccc
     7261 cttccgaaat ggcataggcc gaaacggctg tgtcgccgct gttttcggta cggctcaccg
     7321 tgtaacctga tgcaagggtg taaaccgatg aagcattgta tccgtttact ttatttctgc
     7381 tactcaattc tgttaacgaa cctatctgag cattgtcgat gctgaaatca gaagtgagac
     7441 ggtattcatt gccggctttc cggtttctga acacataata atcggtcacc ttagcttcga
     7501 agttgtttgt gagggccgtg tcggctttct gatgccagaa catggcagcc ggatgacgaa
     7561 ccttttgtac aggtaaacgt acaatcatgt attcactttc gcctgttttg gtgaaaaaac
     7621 gataatacgg attattcgtt ttaacgcgtt gccaggaata gtcataaatg cctgccactt
     7681 tatccagtgt tatcattact gtatcattcg gcatgaaata ccactccgaa aaagcgggtg
     7741 ctttggccat tccggtagca gcgctttcat cgttttgact caaaagctga aaatcggctg
     7801 aagtctttat tttatcaaca gcagcattca gttcttttac tcctttatcg agcgattctt
     7861 tgagcgatac acctgccggc tcagaatcgc atgatactcc aatgaccaca aaaagtaaag
     7921 cgattacaga atttaataat gttgacttca tacatttcat ttgttttaat aatgattatt
     7981 ttatgaatta tatttttctc tcaaaatatt taccggacat tttattctat aat
//
LOCUS       DAORKP010000107         7974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1440_length_7974_cov_9.314307, whole genome shotgun sequence.
ACCESSION   DAORKP010000107 DAORKP010000000
VERSION     DAORKP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_7974_cov_9.314307"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..455)
                     /locus_tag="PLN75_06850"
     CDS             complement(57..455)
                     /locus_tag="PLN75_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03870.1"
                     /translation="MKRKAYCIHNYFIVAGVVLIVAGLIAIYFFIVKGIKPEFLQLDV
                     FTVYSKYLETKTFTLIKNNQGDEIAFTLYWTGWLMIVYGLRKSLMNKESLAIIILIAG
                     YVLLHGLAAMYFLLCFLFVLPLFFVIKKVK"
     gene            832..1908
                     /locus_tag="PLN75_06855"
     CDS             832..1908
                     /locus_tag="PLN75_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRG03871.1"
                     /translation="MKENNYCIIMAGGLGSRFWPFSRNERPKQFLDFFGTGRSLLQMT
                     FDRFRNVVPASNILIVSNIIYRNLILEQLPEIKSEQVLLEPTRRNTAPCIAYAVNRIK
                     ATTSTANIIVAPSDHLIMKEERFIETIQEGLDFVEKNNSLLTLGIKPSRPETGYGYIQ
                     VGEGTENPRKVKTFTEKPNAELAQVFFETGEFFWNSGIFLWNLKTIDSAFNIYLPEVA
                     MKFNAGKDFYNTEKEQDFIDEMYPSCPNISIDYGIMEKAESVYVLCAEFGWSDLGTWG
                     SLYDLSAKDDNQNVILKCDTIMYDSNENIIALPKDKLAVIQDLNGYIVAESDNVLLIC
                     KRSDEQRIRQFVNDANFKFKGNYS"
     gene            2059..3897
                     /gene="mutL"
                     /locus_tag="PLN75_06860"
     CDS             2059..3897
                     /gene="mutL"
                     /locus_tag="PLN75_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRG03872.1"
                     /translation="MTDIIHVLPDSVANQIAAGEVIQRPASVVKELVENSIDAGAKNI
                     QVIIKDGGNALIQIVDNGKGMSETDARMSFERHATSKIRQAEDLFSLRTMGFRGEALA
                     SVAAVAQVEMRTKREDDVLGTLIEIAGTRVFRQESVQCDTGTNISVKNLFFNVPARRR
                     FLKSQSTEMMHVRNEFFRIVLVHPEISFSLFEDDKELMVLPVSSLKARIENVFVMFSK
                     KRMDQQLLPVEAATSLLNIRGFVGRPEFAQKNAQQYFFVNERYMRHPYFHKAVMVAYE
                     KLIKPGENPNYFIYFDIDPNSIDVNVHPSKTEIKFDNEQAVWSIILATVKEALGKFQV
                     MPPLDFDQEDSPDIPVLDKTTRIVPPRINFDPNYNPFGKTSTTSKRPDYDWEKLYDDF
                     NKNKEKGKAPSATANTEWVDAEIPIQQQLEITDASADYLQFKLKYILTSVKSGLMLID
                     QHRAHFRVLFDRYMLIIRNQKGVSQQMLFPEVLELETGEVVIIERILPELLWIGFDIE
                     NFGKNAYVINGIPAEMGAGSVLSLLKELIAEVQLTELSVSETIQEKIASVMARKTAIQ
                     YGKSMTLTEMTHLFQDLFSCDNHQYAPDGKPIITVLLNEEIEKRFR"
     gene            complement(3979..4686)
                     /locus_tag="PLN75_06865"
     CDS             complement(3979..4686)
                     /locus_tag="PLN75_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRG03873.1"
                     /translation="MNEEKVRILLCEDDENLGMLLREYLQTKGYDCDLQPDGEAGYKA
                     FTRNKYDLCVLDVMMPKKDGFALAADIRAMNIEVPIIFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESILRRVRGKKSKDVVSYQIGGFTFDAQKQTLEFDGKAKKLTT
                     KESELLNLLAANANNILERNFALKTIWVDDNYFNARSMDVYITKLRKLLKDDPNVAII
                     NIHGKGYKLIMPQVKED"
     gene            complement(4691..6208)
                     /locus_tag="PLN75_06870"
     CDS             complement(4691..6208)
                     /locus_tag="PLN75_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRG03874.1"
                     /translation="MKKSTLWLLIGLMAVTFGALIALQVRYLSATAALAEKQFDDGVK
                     RSLIQTVTLLEENEALKFLAQTLEDDKYIQTNRSSNSYRKPSTDLRITDSVRRDWNKD
                     PLTPSIRLSTGHGKATIEETSKFLREKFQLNFSRSKTILDQAVFRWLHESENKDINER
                     VDFLELDEILDTYFKSNGIELPFYYTVVDKRNKVIYQCNKTINVNGSPNTDVYVQKLF
                     PREDTQRETFIQIVFPTKQNFIFESLNLLLPSIGVVLLTLGIFVITIIIIFRQKQLNT
                     IKNDFVNNMTHEFKTPISTISLASQMLQDTEVGKNPAMLKHVSNVIKDETKRLSLQVE
                     KVLQMSMFENEKATLKLTEIHINNLIKDIISSFSLKVTSKGGEINTSLKAQNDLLLID
                     EVHFTNVIFNLMDNALKYCDKSPILSIETWNEKENLCISVEDNGIGIKKENIKRIFEK
                     FYRVSTGNLHNVKGFGLGLAYVKKIVNDHRGTIKVESELGLGTKFTIIIPTLKNY"
     gene            6444..7697
                     /locus_tag="PLN75_06875"
     CDS             6444..7697
                     /locus_tag="PLN75_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG03875.1"
                     /translation="MEQNRQKWFSLFICNFFGVFNDNFLKNSIIFVTIAWSLPTWLSH
                     SQLISIVSSCLVIPYLIFSPLGGRFAVRYSKLKVFRVLKLIEIPIMMIACVAFFKHWI
                     VLAIVSMLIMGIQSSLYSPSKYSLIRDIGGEEGASFGSGMFETMAFLGILLGTFVASV
                     VSDHFSLWLFSSVILGIALMGYLSSKTIKAVELPVEKTGSETLNPIRFVRQNYLFARE
                     HKLVNSAIIGSSSFWLISGMVQMNVIIHCKNVYGVSNSLTGLVMAFAAIGIAAGTSVT
                     GKYSGTVVRKGLIIPALCGMILNLVLLLIVPLSFGTFAAGIVVFAFIAGVFQVPCMAM
                     MQQADLGRKRGDTIAYLNMMNFLFILAGTGLFSLTTALTGENSYAVFGVMIAVCAVIL
                     LYFAFRYPDFRTETRLLFEKSGNQL"
     gene            7736..>7974
                     /locus_tag="PLN75_06880"
     CDS             7736..>7974
                     /locus_tag="PLN75_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03876.1"
                     /translation="MNKKIRFAISFLIVVLALTACNQSSKTSEGDGNHFSQTEKTNAE
                     IQPNTAAEIYAKKQVPVLCYHRIENGRNDEYTVSPA"
BASE COUNT         2364 a         1465 c         1593 g         2552 t
ORIGIN      
        1 cttcggaatc attttttttt gtacaaccag taaatctgag tttttaaaac gaggttttac
       61 ttcacttttt tgataacaaa aaacaagggt aatacgaaaa gaaaacacaa caaaaaatac
      121 atagcagcca gtccgtgcag caaaacatat cctgcgataa gaataatgat agcaagtgat
      181 tccttgttca tcaatgattt tcttagaccg taaactatca ttaaccagcc tgtccagtaa
      241 agtgtgaagg caatttcatc cccctgattg tttttaatca atgtgaaggt ctttgtttca
      301 agatacttcg aataaacggt aaatacatcc agttgtagaa attcgggttt gattcctttg
      361 acgataaaaa aataaatagc tattagtcca gccactatta aaacaacgcc ggcaacaatg
      421 aagtagttgt gtatgcagta cgcttttctt ttcatactgt aaaaatgttt cggttaagat
      481 aaaaaattga agaaataatc gctgattgca aagtaaagaa ttttttttga atgataatga
      541 atattacatt cggttattca cgcgtaatgc tgataatata tgtgtgtgac aaataatatt
      601 ttacagattt ataaattgta ttcagataaa taaatcagga taattttgaa aaagaaatga
      661 caataaaaat tatctctgaa acctgctcat aaaaagcatt ttaataaata tttttcagct
      721 tcatagtgaa atttatgcga tatttcggac attttatttg ttcattctaa aaataaaacg
      781 taatttagtg caacgttttt gtgttaaata taaaaagctg aaattcaaaa catgaaagaa
      841 aataactatt gtataatcat ggcgggaggg cttggttctc gtttctggcc attcagcagg
      901 aatgaaagac ccaagcagtt tcttgatttt ttcggtaccg gtcgttcgct gttgcaaatg
      961 acttttgacc gtttcagaaa tgtggttcct gcttcaaata ttctgattgt ttccaatata
     1021 atctacagga atctgattct ggaacagtta cccgaaatta aatccgagca ggtgcttctg
     1081 gaaccgacaa gaagaaatac agcaccctgt attgcatacg ctgtaaacag aataaaggct
     1141 acaacctcca ctgcaaatat cattgtagct ccctctgacc acctgatcat gaaagaagaa
     1201 cgttttatcg aaacaattca ggaaggactg gattttgttg agaaaaacaa cagtctgctc
     1261 acactgggta ttaagccgag cagacctgaa acaggctatg gctatattca ggtcggagaa
     1321 ggaaccgaaa atccacgcaa agtgaaaact ttcactgaga aaccaaatgc agaactcgca
     1381 caggtttttt ttgaaacagg tgaatttttc tggaattcag gtatttttct atggaatctg
     1441 aaaacgattg acagcgcttt caatatatac ctgcctgaag ttgcaatgaa atttaatgca
     1501 ggaaaagatt tttacaatac tgagaaagag caggatttca tcgatgaaat gtatccctca
     1561 tgcccaaaca tctctattga ctacggaata atggagaaag cagaaagtgt gtacgtgcta
     1621 tgcgccgaat tcggttggtc tgacttaggt acgtggggtt cactttacga tttatcggct
     1681 aaagatgaca atcagaatgt aattcttaaa tgtgatacca taatgtatga cagcaacgag
     1741 aatatcatag cactacccaa agataaactg gctgtaattc aggatttaaa cggttacatc
     1801 gttgccgaat cagacaatgt acttttaatc tgtaaacgat cggacgaaca acgtatcagg
     1861 cagtttgtca acgatgctaa cttcaaattt aaaggaaatt attcttagtg attttttcaa
     1921 acagagtttg ccggcaaggc aagctctgtt gattatcagt gaaatagtaa gtgcaatttg
     1981 ttcagattaa gattttttct ttttcggaat aaagaatctt atattcactt aatttttcaa
     2041 atccaccatc cacttttcat gactgacata atacatgtac tgcccgattc ggttgccaat
     2101 cagatagcag ccggcgaggt gattcagcga ccggcttctg tagtgaagga actggtagag
     2161 aattcgattg atgccggtgc taaaaatatt caggtgataa ttaaagacgg aggaaatgcg
     2221 ctcattcaaa tagtagataa cggcaaggga atgagtgaaa ctgacgcacg aatgtcgttc
     2281 gaacggcatg ccacttctaa aataaggcag gcagaagatc ttttttcgct ccgaacgatg
     2341 ggattccgcg gcgaagcact cgcatcggtg gcagcagtag cacaggttga aatgcgaaca
     2401 aagcgtgaag acgacgtatt gggcacgctg atagaaattg ccggtacccg cgtattcagg
     2461 caggaatcgg ttcagtgtga tacaggaacg aacatttcgg tgaaaaatct ttttttcaat
     2521 gttcctgcac ggcgtcgttt cctgaaatct cagtccacag aaatgatgca tgtacgtaat
     2581 gaattctttc gtattgtgct ggtacatccc gaaataagct tttcattgtt tgaggacgat
     2641 aaggagctga tggtattgcc tgtatcgtca ctgaaagcga ggattgaaaa tgtttttgtt
     2701 atgttttcaa aaaaaaggat ggaccagcag ttgcttccgg tagaggcggc cacaagtctt
     2761 ctgaatattc gcggattcgt gggtagaccc gaatttgccc aaaaaaatgc tcaacagtat
     2821 ttctttgtaa atgaacgata tatgcgtcat ccgtactttc acaaggcggt aatggtagcg
     2881 tacgaaaaat tgattaaacc gggtgaaaat cccaactatt tcatttattt cgacattgat
     2941 ccgaattcca ttgatgtgaa cgtacatccg tccaagactg aaataaaatt tgataacgaa
     3001 caggctgtct ggtcaatcat ccttgctacc gttaaggaag cccttggcaa gtttcaggta
     3061 atgccgccac ttgattttga ccaggaagat tcgcccgata ttcctgtgct ggataaaact
     3121 acccgcatag ttcctcccag aatcaatttt gatccgaatt acaatccgtt cgggaaaaca
     3181 tccactactt caaaaagacc tgattacgac tgggaaaaac tctacgatga tttcaataaa
     3241 aataaagaaa aaggaaaagc cccgtccgct acagcaaata ctgaatgggt ggatgctgaa
     3301 attccaattc aacagcagct tgaaataacc gatgcatcgg cagattatct gcagttcaaa
     3361 ttgaaatata tacttaccag cgtaaaatcc ggactcatgc tgattgacca gcaccgggca
     3421 catttcagag tcctttttga ccggtatatg ctcattatac gcaatcagaa gggtgtgtct
     3481 cagcagatgc tttttcctga agttctggaa ctggagaccg gagaagtggt aattattgag
     3541 cgaattcttc ccgaattact ttggataggg tttgatattg aaaattttgg gaaaaacgcg
     3601 tatgttatta atggtattcc ggctgagatg ggtgcaggat cggttttatc cttgctgaaa
     3661 gagctgattg ccgaagtgca actcaccgaa ctaagtgtaa gtgaaaccat tcaggaaaaa
     3721 atagcgtcgg tgatggcacg caaaacagct atccaatacg ggaaaagtat gacactgact
     3781 gagatgactc acttgtttca ggatttgttc agttgtgata atcatcagta tgctccggat
     3841 ggtaaaccaa ttattactgt attactaaac gaagaaattg aaaagcgatt caggtaaaat
     3901 gagcaggaga gtaaaggaac aaaaaaaatg cggtgaaatt caccgcattt ttttgtaatt
     3961 gatattattt cgttgttatt agtcctcttt cacttgtggc ataatcaact tataaccttt
     4021 gccatgaatg ttgatgatgg ctacattcgg atcatctttg agcaatttgc gcaatttggt
     4081 aatgtatacg tccatgcttc tggcattgaa gtaattatca tctacccaga ttgttttcag
     4141 tgcaaaattg cgttccagga tgttgttggc atttgcagcc agcagattaa gcaattcaga
     4201 ttctttggta gtcagctttt tagctttgcc gtcaaattcg agtgtctgct tttgtgcatc
     4261 gaaggtaaat ccaccaatct gataggatac cacatctttt gatttttttc cgcgaactct
     4321 gcgcagaatt gattcaatcc gatatagcag ttcttccatg ctgaatggtt ttgatagata
     4381 atcgtcagca cccagtttga atccctgcag tatgtcttct ttcatggact tagcagtaag
     4441 gaaaatgata ggcacttcga tgttcatagc tctgatgtca gctgccaggg caaagccgtc
     4501 tttttttggc atcattacat ccaacacaca caagtcatat ttattgcgtg taaacgcttt
     4561 ataaccagct tcaccgtcag gctgcagatc gcagtcatac ccttttgttt gcaggtattc
     4621 cctcaataac atgccaagat tttcgtcgtc ttcgcatagt aagattctta ctttttcttc
     4681 gttcataatt ttagtaattt ttaagcgttg gtattattat cgtaaatttt gttccgagac
     4741 cgagttcact ttcaactttt attgttccgc gatgatcatt cactattttt tttacataag
     4801 ccaatcctaa accgaatcct ttgacgttgt gaagattgcc tgtcgataca cggtaaaatt
     4861 tttcaaaaat ccgttttatg ttttcttttt taattccaat tccgttgtct tctacactga
     4921 tacacagatt ttctttttcg ttccatgttt caatacttaa aataggcgat ttgtcgcagt
     4981 atttcagagc attgtccatc agattaaaaa taacgttagt gaaatgcact tcgtcaatca
     5041 gcagcaggtc gttttgagct ttaaggcttg tattgatttc gcctcctttg ctggttactt
     5101 tcagagaaaa actactgata atatctttaa tcaggttgtt aatgtgtatt tcagtcagtt
     5161 tgagtgttgc cttttcattc tcaaacatag acatttgcaa tactttctcc acctgaaggc
     5221 tcaaccgttt cgtttcatct tttatgacgt tggacacatg tttcagcatc gccggatttt
     5281 taccaacttc ggtatcctgc agcatttgcg aagcaagtga aatggttgaa atcggggttt
     5341 taaattcatg cgtcatatta ttaacaaaat cattcttaat agtgttcagt tgtttctgcc
     5401 tgaaaatgat tataatagta ataacaaaaa tgccaagtgt aagcaggact actcctattg
     5461 agggtaaaag cagattcagc gattcaaaaa taaagttttg ttttgtcggg aaaacaatct
     5521 gaatgaacgt ttcccgttgt gtatcttcac gcgggaacag cttctgaaca tacacatctg
     5581 tgttcgggct gccatttacg ttgatagtct tgttgcactg atagattact ttatttcttt
     5641 tgtcaactac ggtgtagtag aaaggcagtt cgatgccgtt gctcttgaaa taggtgtcca
     5701 gtatttcgtc cagttccaga aaatcaacgc gctcgttgat atctttgttc tccgattcgt
     5761 gcagccacct gaatacggcc tgatcgagaa ttgtttttga acgtgaaaaa ttaagctgaa
     5821 acttttcacg caggaatttg gatgtttcct cgattgtagc tttcccgtgt cccgtcgaga
     5881 gccttattga gggtgtcaat ggatctttat tccagtctct tcgcactgaa tcggtaattc
     5941 tcagatcagt cgaaggtttc ctgtatgaat tactgcttct gttggtctga atatatttat
     6001 catcttccaa tgtttgtgcc aaaaatttca atgcttcgtt ctcctcgagc aaggtaacag
     6061 tctgaatcag gcttctcttt acaccgtcat cgaactgctt ttcagcaagt gctgcggtgg
     6121 cggataagta cctgacctgc aatgctataa gcgctccgaa tgtaactgcc atcagcccta
     6181 ttaataacca aagcgtactt tttttcatga ctttacaaag gtagaataaa acgaaatgtt
     6241 aatttaacat ttaacacaat ttaactcgac aatttattaa cattagtgat ttgccagtcg
     6301 attagccgat tgatgttcaa caaatctccg attattatat ttaacgtaaa taaaataaat
     6361 tttgtttatt caatttattt ataaattttt acttaaaaca atgattcatt atttgtaatt
     6421 ttgcattcaa gatgagtttt gctatggaac agaacagaca aaaatggttt tcgcttttta
     6481 tttgtaattt ctttggagtt ttcaatgata attttctgaa gaattccata atttttgtca
     6541 ccatagcatg gtctctgcct acgtggttgt cgcattctca gttgatttcc attgtaagtt
     6601 cctgtctagt tatcccttac ctgatatttt ctccgcttgg cggaagattt gctgtacggt
     6661 attcaaaact gaaagttttt cgggtgctca aattgattga aattcccatc atgatgattg
     6721 cgtgcgtggc atttttcaaa cactggatag tgcttgccat tgtttctatg ctgattatgg
     6781 gtattcagag ttcgctttat tcaccctcca agtatagttt aatacgtgat ataggtggcg
     6841 aagagggagc ttcgttcggt agtggcatgt ttgagacgat ggcttttctg ggcattttat
     6901 tgggaacatt cgttgcatcg gttgtttcag atcatttttc tttgtggctt ttttcttccg
     6961 tgattttggg aattgcactg atgggttatt tatcttcaaa aacaatcaaa gcggtggagt
     7021 tgccggtcga aaaaactggt tcggaaacgc ttaatccgat tcgcttcgtc aggcaaaatt
     7081 atctttttgc cagggaacac aaactggtaa acagtgcaat tatcggttca tcttctttct
     7141 ggcttatttc aggcatggtt cagatgaatg taattattca ctgtaaaaat gtatatggag
     7201 tttccaactc gctcacaggt cttgtgatgg catttgcggc cattggtata gcggcaggaa
     7261 cttcggtaac gggtaaatac tcgggcactg tcgttagaaa aggattgata atacctgccc
     7321 tgtgtggtat gattctgaat ctggttttat tattgattgt gccattatct tttggaacgt
     7381 ttgcagcggg tattgtagtt tttgcattca tagccggagt ttttcaggtt ccatgcatgg
     7441 ctatgatgca gcaggctgat ttgggaagaa aaagaggaga tacgattgca tatctgaata
     7501 tgatgaattt tttatttatt ctggccggaa ccggtttgtt ttcacttaca acagctctga
     7561 ccggcgaaaa tagttatgcc gtatttggag ttatgattgc ggtttgtgct gtaattctcc
     7621 tctatttcgc attcagatat cccgatttca gaacagaaac acggctcttg ttcgaaaaat
     7681 ctggtaatca attataaata gtttttttaa aaattttgaa aagataagat atagtatgaa
     7741 caaaaaaata cgctttgcta ttagcttttt aattgttgtt ttagcattaa cagcatgtaa
     7801 ccagtcatcc aaaacttcag aaggtgatgg taatcatttt tcgcaaacag aaaaaaccaa
     7861 tgcggaaata caaccgaata ctgcagccga aatttatgcc aaaaagcaag tgcccgtgtt
     7921 gtgctaccac cgaattgaaa acggacgaaa cgatgaatat accgtatctc cggc
//
LOCUS       DAORKP010000108         7897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1475_length_7897_cov_9.984698, whole genome shotgun sequence.
ACCESSION   DAORKP010000108 DAORKP010000000
VERSION     DAORKP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_7897_cov_9.984698"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1374
                     /locus_tag="PLN75_06885"
     CDS             226..1374
                     /locus_tag="PLN75_06885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRG03877.1"
                     /translation="MTNSQISAAAFSDTKPHYAILDGLRGVAAIMVIFYHVFEAFATS
                     PFDQRFNHGYLAVDFFFILSGFVIGYAYDDRWKKMTVKDFFKRRLIRLHPMIVMGFVL
                     GALTFVIQGSEKWDGTPTPVSMIMLATLLSLFLIPAYPGAGYEVRGNGEMFPLNGPSW
                     SLFFEYIGNILYALILRRMGTKALTVLVALAGAGLASFAVFNFSGAGHLGVGWTLADN
                     NFLGGSLRLMFAFSAGLLMSRVFKPVNIRGAFWISSLIIVVLLSMPYISSKATPWMNG
                     IYDSVCVILVFPLVVYLGASGKTTDKFTTRLCKFLGDISYPLYMVHYPFMYLYYSWVW
                     KNELTFAQTWHVAILLVVGNILLAYLVLKLYDIPVRNFFTKRLMNRQK"
     gene            complement(1471..3066)
                     /gene="pckA"
                     /locus_tag="PLN75_06890"
     CDS             complement(1471..3066)
                     /gene="pckA"
                     /locus_tag="PLN75_06890"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRG03878.1"
                     /translation="MANLDLSKYGISGDIEILHNPTYEELFQAEIDPANEGFEKGVLT
                     KTGAVAVDTGKFTGRSPKDKFIVMDDTTRDTLWWDGTINRPTTPEVFDHCKDLVTAQL
                     STAKKLYVVDTFCGTNEDTRMKVRFIMEVAWQAHFVTNMFIRPSHYELANYGEPDFVC
                     LNGSKTVNPNWKEQGLNSEVFTLFDLTRKMQVIGGTWYGGEMKKGIFALMNYYLPLRG
                     IASMHCSANVGAEGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGVFNYEGGC
                     YAKVIDLEKDKEPDIWRAIRRDALLENVTVLSDGTPDYTAAASKTENTRVSYPIYHIS
                     KIVSPSRAGHAKKIIYLSADAFGVLPPVSILDDKSAQYHFLSGFTSKLAGTERGITEP
                     VPSFSPAFGEAFLTLHPTMYAKTLIGKAQEHNAKVYLVNTGWNGTGKRISLKNTRAII
                     DAIIDGSIENAPTTHIPILNLVAPTALNNVDTEILDPRNTYADVTEWETKAKSLAAKY
                     LKNFEQYCDNDDALALVASGPQL"
     gene            3429..5030
                     /locus_tag="PLN75_06895"
     CDS             3429..5030
                     /locus_tag="PLN75_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03879.1"
                     /translation="MKTKLMSMLAFVVAAFTFSANAQSYGYNDYDDYNSSGRYSNRNV
                     TVEAESSDISYNLDLRAVANVFADSRDLEEFEQRINDYNTGINNLDLNADGQVDYLRV
                     IESAKGNTHLVVLQAVIGYDMYQDVASIVVEKRNNKRHYVQVIGDPYIYGPYYIIEPV
                     YVYTPSIFSFFWGPRYFRWHSPYYWGYYPSYWHFWNPYPLGVYVNNINIFCNNYNHHY
                     NYSDRIISNNYNELRREVSRNDFQRSNPDGSFINRNSSRSNVTNRRDLGSYRGSDRAA
                     VSSRDNSSARSEAGQRTRGTSMDARSNTRNSAGSVRGTYNENQRMNSGRSTGAVREGY
                     NGAQRSSGNTRMDQNTRNGEYNRGAVRSNSGRSQDNNGSYSPRSNSGSSRSTSSYETP
                     GRASNSPTVAPRGSNSEGRSSTYSQPGRSSDSRSGSYSAPSRSSSGGTYSAPSRSSSR
                     ESYSTPSRSSSRSSSTYSQPSRSSSGGSYSAPSRSSSSSSGSYSAPSRSSSSSSGSYS
                     APSRSSSSGSSSSRSSSGSGGRSSR"
     gene            complement(5274..6926)
                     /locus_tag="PLN75_06900"
     CDS             complement(5274..6926)
                     /locus_tag="PLN75_06900"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRG03880.1"
                     /translation="MLKKTIIAWVALGLLSIAHSTACTNFLVGKNATTDGSTFISYAA
                     DSYYLFGALYHYPASVNAPGTMRDVYEWDTGKYLGKITEAERTYNVIGNMNEYQVSIG
                     ETTFGGRSELIDTTAIIDYGSLMYIALQRSRTAREAIKIMTDLVAEYGYYSSGESFSI
                     ADPNEVWIMEMISKGPAEKGAVWVALRIPDDCVSAHANQARITTIPFKDKNNCLYSKD
                     VVSFARKKGYFTGKDSEFSFSDVYNPLDYSGLRICESRVWSFFRQVNPDMDRFITYVK
                     GETKERMPLWVKPTKKLDKNDITGFMRDMYEGTELDMTKGFAAGAFGSKLRHSPLTFK
                     VDGVEYGHERPIATQQTGFSFVSQMRSWLPHYMGGILWFGVDDAASNVYVPVYCTTDK
                     VPWCFDEKNGSLLDYSSSSAFWIFNQVSNFAYGKYLPMMQDIKMKQKELESAFDKDQP
                     EVEKSASAMSEAEARKFLTDYSLRAAEKSTDQWKKLSEFLLVKFMDGNIKKEENGKFV
                     KNQYGLPPTIIRAGYSDECKLQMVKENPGLRQKTQAELDSRK"
     gene            complement(7422..7841)
                     /gene="mscL"
                     /locus_tag="PLN75_06905"
     CDS             complement(7422..7841)
                     /gene="mscL"
                     /locus_tag="PLN75_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRG03881.1"
                     /translation="MGFVKEFKEFAMKGNVIDLAVAVIIGGAFGKIVSSFVADIVMPL
                     IGLLLGGVNFTDLKWVIKDAVMENGVEKAAAATLNYGNFIQVTIDFLIIALAIFLAIK
                     AMNSLKKKEEPAPEAPAAPSAEETLLGEIRDLLKEKK"
BASE COUNT         2168 a         1754 c         1675 g         2300 t
ORIGIN      
        1 acagaatagc aaagtgaaaa gacttctgct tgtgttttca gccgatttga aaatcggctg
       61 tttcatgata aaaaaacaat ctttttaata ttgttttaga aaagattggt ttattcattg
      121 tcttattttt aaaatttaaa ttttattcca aaatatatgc ggtcagattt ttgtactttt
      181 gtatttcttt tttaaaaaca gccttttgaa atctgacatt gcactatgac aaactcacaa
      241 atctctgccg ctgccttttc tgacaccaaa ccacattacg ccatactcga cggactacgc
      301 ggtgtagctg ccatcatggt cattttttac cacgtattcg aggctttcgc taccagcccg
      361 ttcgaccagc ggttcaatca tggctacctg gctgtcgact ttttctttat tctttccgga
      421 ttcgtgattg gttatgccta cgacgaccgc tggaagaaaa tgaccgtcaa agattttttc
      481 aaacgccggc ttatccgttt gcaccccatg attgtgatgg gatttgtgtt gggtgccctc
      541 accttcgtga ttcagggcag tgaaaaatgg gacggcactc ccacgcccgt ttccatgata
      601 atgcttgcca cgctgctcag tcttttcctg atacctgcgt atcccggagc gggctacgaa
      661 gtgcgtggaa atggcgaaat gtttccgctc aacggtccct cctggtcgct cttcttcgaa
      721 tacatcggga atattctgta tgctttgatt cttcgccgaa tgggcaccaa agcacttacg
      781 gtgctcgttg cgctggcggg tgcgggattg gcttcgtttg ctgtattcaa tttttccggt
      841 gccggacatt taggcgttgg atggacgctg gctgataaca atttcctggg cggttcactc
      901 aggctgatgt ttgcattttc tgccggactg ctcatgtcac gtgttttcaa acctgtaaat
      961 atccgcggag cattctggat tagcagtttg attattgtcg tcttgctatc aatgccttac
     1021 ataagcagca aagcaactcc gtggatgaat ggcatctacg actccgtttg cgttattctt
     1081 gtttttccgc tggttgtata tctgggcgct tcaggcaaaa ccaccgataa attcactacc
     1141 cgtctgtgca aattcctggg cgacatttcc tatccgctct acatggttca ttatccattc
     1201 atgtatcttt attactcatg ggtgtggaaa aacgaactta catttgcaca gacctggcat
     1261 gtagccatac ttttagtggt gggtaatatc ctgctggctt atctggtgct caaattgtat
     1321 gacattccgg tacgaaattt tttcacaaaa agactgatga acaggcaaaa ataaaagcaa
     1381 ttaatatata tagctacctc tgaaatgatt aacaaaaaat cccgctccaa atgctgaagc
     1441 gggatttttt taatatagtt acaactcaaa ttacaattgc ggacctgaag caaccaatgc
     1501 aagtgcatcg tcgttgtcgc aatactgttc gaagtttttg aggtatttag cagccagtga
     1561 ttttgctttt gtttcccatt ctgtcacatc ggcataggta ttgcgtgggt cgagaatttc
     1621 tgtatccaca ttgttcaatg cagtaggagc cacgagattc agaattggaa tgtgcgtagt
     1681 cggagcattt tcgattgaac cgtcgataat ggcgtcgatg atggcgcggg tgtttttgag
     1741 cgaaatacgt tttccggtac cgttccagcc ggtgtttacc agatacactt tagcattgtg
     1801 ctcctgagct tttcctatca gggttttagc atacattgtt ggatgcagtg tgaggaatgc
     1861 ttctccaaat gcaggagaga atgatggaac aggctctgta ataccgcgtt ctgtaccggc
     1921 cagtttggag gtaaagcccg aaaggaagtg gtattgagca gatttatcat ccaggattga
     1981 taccggaggt aacactccga aagcgtcagc cgaaaggtag atgatttttt tggcatgtcc
     2041 tgcgcgtgaa ggagaaacga ttttgctgat atgatagatt ggataggaaa cacgtgtgtt
     2101 ttctgttttt gaagcagcag cggtgtaatc aggagttccg tcggaaagta cagttacgtt
     2161 ttctagcagt gcatcgcggc ggatagctct ccagatatcg ggttctttgt ctttttccag
     2221 gtcaatcact ttggcgtagc aaccaccttc gtagttgaac acaccgtgat cgtcccatcc
     2281 gtgttcgtcg tcaccgatga ggtagcgttt cgggtcggct gaaagggttg ttttacctgt
     2341 tcccgagagt ccgaagaaaa tggcaacgtc gccttcggcg cctacgttgg cagagcagtg
     2401 catcgaagca ataccgcgaa gtggcaggta gtagttcatc agtgcaaaaa tacctttttt
     2461 catttcaccg ccgtaccagg taccgccgat tacctgcatt ttgcgggtaa ggtcaaacaa
     2521 agtaaacact tccgagttca atccctgttc tttccagttt ggattcactg ttttcgatcc
     2581 attcagacat acgaaatcgg gttcgccgta gttagccagt tcgtagtgcg acggacggat
     2641 aaacatgttg gtcacaaagt gtgcctgcca tgctacttcc atgatgaaac gtactttcat
     2701 gcgggtatct tcgttagttc cgcagaatgt atccactacg taaagttttt tggctgtaga
     2761 aagctgagct gtaacaagat ctttgcagtg gtcgaaaact tcaggagtag tcgggcggtt
     2821 gatggtacca tcccaccaca aagtgtctct tgtagtgtca tccattacga tgaacttgtc
     2881 tttaggagaa cgtccggtaa atttaccggt atccactgca acagcgccgg ttttggttaa
     2941 tactcctttt tcaaaacctt cgtttgcagg atcaatttct gcctgaaaca gttcttcgta
     3001 ggtcggattg tgtaggattt cgatgtcgcc cgaaatgcca tacttactta aatctaaatt
     3061 tgccatttta aaaaatttaa ttgattttat cttgatttta tctctcttct gatttgacgg
     3121 tgcaaaagta ctacaatttc tgcaactaaa aaagcaatta aagaaatttt tctttaatta
     3181 aatttttcac atcacacagg taaactttat taacaggtca aacaataatt attcaaagtt
     3241 tgttcgatcc gaaacaatgt ttaaataaat cttaacaaag gaaaaaacgc ccttcgtccc
     3301 aaaattaact ttttatctat cagcaattta acaactcatt tttgttttgg cacggatatt
     3361 gtttttcaag cggtacccga tcagttgtcc gggaaaaaat cattcacaca gataaaaaac
     3421 acaaaactat gaaaactaaa ttgatgagta tgttagcatt cgttgttgct gcatttacat
     3481 tctcagccaa cgctcaatca tacggataca acgattacga tgattacaac agttcgggcc
     3541 gctactccaa ccgcaatgtt acggtagagg ccgaaagttc cgacatcagc tataatctcg
     3601 acctgagagc tgtagccaat gtttttgcag actcgagaga tctggaagaa ttcgaacagc
     3661 ggattaacga ttataataca gggatcaaca atctcgatct gaatgctgac ggtcaggtag
     3721 attaccttcg cgtaatagaa agcgcaaaag gtaacaccca cctggtagtg ctgcaggccg
     3781 ttatcggata tgacatgtat caggatgtgg cttccattgt ggtagaaaag agaaataaca
     3841 aaagacatta tgtccaggtg ataggcgatc cttatattta tggtccgtat tacatcatcg
     3901 aaccggttta cgtgtacact ccgtctatct tttcgttctt ctggggtcct cgctacttca
     3961 gatggcactc accctactat tggggctatt atccctctta ctggcatttc tggaatcctt
     4021 acccattagg agtgtatgtt aacaacatca atatattctg taataattac aatcatcact
     4081 acaattactc cgaccgtata atcagtaata attacaacga attgcgccgc gaagtgagtc
     4141 gtaacgactt tcagagatca aatcccgatg gttcgttcat caacagaaat tcatccagat
     4201 ccaacgtgac taaccgcaga gacctgggca gctacagagg ctccgacaga gctgcagtaa
     4261 gttcacgcga caattcatcg gcaagatcag aagccggaca gcgcaccaga ggcacatcga
     4321 tggatgcacg gagcaatacc agaaattcag caggctccgt acgcggcacc tacaacgaga
     4381 atcaacgcat gaacagcggc cggagtaccg gcgcagtgag agaaggttat aacggtgcac
     4441 aacgttcgtc aggtaatacc agaatggatc agaacaccag aaacggcgaa tacaaccggg
     4501 gtgctgtccg cagcaattca ggcaggagtc aggataacaa tggaagctac tcaccccgct
     4561 ccaactcagg cagtagcagg agtacatcat cctacgaaac gccgggcaga gccagcaaca
     4621 gtcctacggt agctccaaga ggcagcaaca gcgaaggacg ttcatccaca tatagccaac
     4681 ccggcaggag ttcagattca agaagcggct cttactctgc accaagcaga agttcatccg
     4741 gaggaactta ctccgcacca agcagaagca gcagcagaga atcatattcc accccgagca
     4801 gaagcagcag caggagttca tctacctaca gtcagcccag cagaagttca tccggtggat
     4861 cgtactcggc tccgagcaga agcagcagct caagcagcgg ttcttactca gctccaagca
     4921 gaagcagcag ttcgagtagt ggttcttact cagctccaag ccgcagctca agctcaggta
     4981 gtagctcaag tcgcagctcg tcaggttcgg gcggcagaag ttcaagataa aaaaggcaaa
     5041 taaactaaac ttaaattata tctgttcgat ttaccataat ggtgatttca attcagcgaa
     5101 gcattaaata tgcttcgctg ttgttttttt aaagaaatga ttgaatacaa ttatgcattc
     5161 agtatctaat tcggtcttat gtaccaaaaa tccacctgtc tgaagatgca cttcaaaaca
     5221 agtggattaa ctgatttttt ttgaaaaacc gaatgaggtt atttcacgat tacttatttt
     5281 cggctgtcga gttcggcttg cgttttttgt cgcaatccgg gattttcttt caccatttgc
     5341 agcttgcatt cgtcggagta accggctcgt atgatggtgg gaggcagtcc gtactgattt
     5401 tttacaaatt taccgttttc ttctttcttg atatttccat ccataaattt cacaaggaga
     5461 aattcgctca gttttttcca ttggtcagtc gatttttcag ctgccctgag ggagtaatcg
     5521 gtaaggaatt tacgagcttc tgcttcactc atggcagaag cactcttttc cacttccggc
     5581 tggtccttat cgaatgccga ttcgagttct ttctgtttca tttttatatc ctgcatcatc
     5641 ggaaggtatt taccgtaagc aaaattggac acctgattaa aaatccagaa agcggatgag
     5701 gatgagtaat ccaacagact tccgtttttt tcatcaaaac accacggcac cttatccgtg
     5761 gtgcaatata ccggcacata cacattgctt gcagcatcat caacaccaaa ccacagaatt
     5821 ccgcccatat aatgagggag ccagcttcgc atttgagata caaacgaaaa accggtctgc
     5881 tgagtagcga tagggcgttc gtgtccgtac tccacgccgt ccaccttaaa tgtgagcgga
     5941 ctatgtctca gtttactgcc gaatgcacct gcagcaaatc ctttggtcat atccaattca
     6001 gtaccctcgt acatatcgcg catgaagccg gtaatatcgt ttttatcgag ttttttggtg
     6061 ggtttcaccc aaagcggcat acgctctttg gtttcgcctt tcacgtaagt aataaatctg
     6121 tccatatcgg gatttacctg acggaaaaat gaccaaaccc tggattcgca aatacgcaaa
     6181 ccggaataat ccagcgggtt gtacacatct gaaaagctga attcgctgtc ttttcctgta
     6241 aaatatcctt ttttacgggc aaaacttacc acatctttgg aatacagaca gttattcttg
     6301 tctttgaaag gaatggtggt aatgcgtgcc tgattggcgt gtgccgatac gcagtcgtcc
     6361 ggaatgcgca atgcaaccca tacagctcct ttttcggcag gacccttgct tatcatctcc
     6421 attatccata cttcgttggg gtcggctatg gagaaggatt cacctgaact gtagtatccg
     6481 tattcggcta ccagatcggt cataatcttg atagcttcgc gtgcagtgcg cgaacgctgc
     6541 agtgcgatat acatcaaact gccgtagtca attatggccg tagtatcaat cagttcgctt
     6601 cttccaccga aagttgtttc gccgatgctt acctgatact cgttcatatt accgattacg
     6661 ttgtaagtcc gctcggcttc cgtgattttt ccgaggtatt ttcccgtatc ccattcgtac
     6721 acatcgcgca tggtgccggg agcattaact gatgccggat agtgatacaa tgctccgaaa
     6781 aggtagtacg agtcggctgc gtaagaaatg aaggtagagc catcggtagt ggcatttttt
     6841 ccaaccagga aattggtaca ggctgttgaa tgagctatgg acagcaaacc caaagccacc
     6901 caggcaataa ttgttttttt cagcataatt aaatttctaa aaaatgaatt aaaaaaaggg
     6961 gcaatatttt tccttaccgg aaatatattg ctccgaatta caaaaataaa cattttccga
     7021 ctattctcca aacctctttc ggagagaaac gcttggaaaa gattggcaag gtaaagaatc
     7081 ctgacagaaa ataagcactc agaaatgtct gaataccttt ttttctcaat tttggagtgc
     7141 gttttgaggt aaacagacaa tgcagccgac ccgtaccagt tatacatttc tacctcaaag
     7201 attttatcgt gaattgtcgg atcgatttga agcaattcag cagcttctct catatcatga
     7261 acattcagaa caaaaataac ccgaatatga atgtcgtttg ttgattatcc tgattttatt
     7321 tctacatcat ttagaaagtt tttccggcga taaatatgta ccaaaaaaaa tcgttccgaa
     7381 agcggcttcc ggaacgattg gatttgataa aaatgctgtg attatttctt ttctttcagc
     7441 aagtcacgta tttcaccaag caatgtttct tcggccgaag gtgctgcagg agcttcggga
     7501 gccggttctt cttttttctt gagtgaattc attgctttga tagcaaggaa aatagccaga
     7561 gcgataatca ggaagtcaat agttacctga ataaagttac cgtaattcag ggtagcagca
     7621 gcagctttct ctactccgtt ttccataacc gcatctttaa taacccattt tagatcggtg
     7681 aaattcacac cacctaacag taatccgatc aacggcatta ctatatcagc tacaaaggat
     7741 gacacgattt taccaaaagc gcctccgata attactgcaa ctgccaggtc aattacattg
     7801 cctttcattg caaattcttt aaattctttt acaaatccca taattttatt ttttagtttt
     7861 ataagtacat ggcaaaaata tattaattaa cttaaat
//
LOCUS       DAORKP010000109         7886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1484_length_7886_cov_9.013791, whole genome shotgun sequence.
ACCESSION   DAORKP010000109 DAORKP010000000
VERSION     DAORKP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_7886_cov_9.013791"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1418)
                     /gene="serS"
                     /locus_tag="PLN75_06910"
     CDS             complement(147..1418)
                     /gene="serS"
                     /locus_tag="PLN75_06910"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRG03882.1"
                     /translation="MLTLKHITENTEDVILHLAKKHFDGKEIINQIVELDERRKTTQT
                     AADTYAAELNALSKEIGILFKQGKQSEANAAKDKTVALKENVNRLTEEKEAIEQKLND
                     LLVLIPNLPHESVPEGKHAEDNVVEKSGGTIPELHKDAVPHWDLAKKYDLIDFELGVK
                     ISGAGFPVYKGKGARLQRALINFFLDSARDAGYLEVQPPYVVNAASGYGTGQLPDKEG
                     QMYHAQLDDLYLIPTAEVPVTNIYRDVILNEEDLPVKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVRIDTPEHSYESLTEMVEYVQILVEKLELPWRILRLCGGDMSFTSA
                     LTFDFEVYSAAQQRWLEVSSVSNFENYQANRLKCRYRNADKKIQLCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALVPYTGFEWIE"
     gene            complement(1646..1912)
                     /gene="rpmA"
                     /locus_tag="PLN75_06915"
     CDS             complement(1646..1912)
                     /gene="rpmA"
                     /locus_tag="PLN75_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRG03883.1"
                     /translation="MAHKKGQGSSHNGRESQSKRLGVKIFGGQFAKAGNIIVRQRGTV
                     HNPGLNIGIGKDHTLFALIDGTVEFRKKRDNKSFVSIKPVESTN"
     gene            complement(1934..2248)
                     /gene="rplU"
                     /locus_tag="PLN75_06920"
     CDS             complement(1934..2248)
                     /gene="rplU"
                     /locus_tag="PLN75_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRG03884.1"
                     /translation="MYAIVEILGQQFKVEQGKKLFVHRMNEAERGSKVEFDKVLLVDN
                     DGAVTVGSPVVDGAKVVCEVVSHVRGEKVIVFHKKKRKGYRKRNGHRQDFTELTIKEI
                     LA"
     gene            2691..5216
                     /locus_tag="PLN75_06925"
     CDS             2691..5216
                     /locus_tag="PLN75_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRG03885.1"
                     /translation="MSVKYSDQLNNVFTYSLQEAERLGNAYVGPEHLFLGLIRNAETH
                     AAELMQAMGVDINSAKQRIEAMVRTDSEPTGADIPMLKSTEKIKKVMELEARSMSAET
                     ADTEHLLLAILKDKNNLAVDVLSADNLTYEKLIKHLNPMKPQDTQASAGLPHEDDEDD
                     EDQYGSRKSKTKQSGNKPSETPALDTFGTDITKAAVENRLDPVVGREREIERIAQILS
                     RRKKNNPVLIGDPGVGKSAIVEGLALRIVQHKVSRVLFDKRVVALDMASIVAGTKYRG
                     QFEERIKAILNELQKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALSRGEIQCVG
                     ATTLDEYRQSIEKDGALERRFQKVMVDPTTPEETLTILQNIQDRYEYHHNVRYTPEAI
                     KACVRLTDRYITDRSFPDKAIDALDEAGSRVHIATVSVPTEIEQLEKRLEDLKAEKNR
                     VIAEQKYEEAVPIRDKEKQTQYQLEDAIKRWEKESQLNPETVDEEKVAEVVAMMSGIP
                     VQRIAQAEGLKLRQMGEVLKSRVIGQNDAVDKITKAIQRNRIGLKDPNKPIGSFLFLG
                     PTGVGKTHLARVLADFMFDSTDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLT
                     EKVRRKPYSIVLLDEIEKAHPDVFNIMLQVMDEGRLTDSLGRRIDFKNTIIIMTSNVG
                     TRQLKDFGRGVGFNMDEDMTIDSDYSRGVIQKALNKTFAPEFLNRVDDIVMFDQLNRE
                     SLKSIIDLELKGIFKRVTDLGYKLELSPEAKDFIADKGYDVQYGARPLKRAIQRYLED
                     ELADVVLAGEAAEGDTILMELNEAKDGVKARVVKQNPTITETE"
     gene            complement(5309..6178)
                     /locus_tag="PLN75_06930"
     CDS             complement(5309..6178)
                     /locus_tag="PLN75_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRG03886.1"
                     /translation="MNSKKDYLKSFYWKDYIMIFAGLILYAVGLTGFLLPNKIVTGGL
                     AGVTVLIKSATEIPLWVSYVSINAVLLIFAWKIVGKNFVIKTVVSVAVLTILLRYGEF
                     YITKPIIHADALLSSMIGAMFCGAGLGLVYSVNGSTGGTDIIGAVVTKYRHVSMGRVL
                     LLVDILIVASSFFLFRSVEKIAIGLIVMAVMYYAVDVMISGMRQSVQFFIFTSKYDEV
                     ASHINSEIKRGCTIIDGMGWYSKKSQKIIIVLARKTESTSIFRLVKSIDQDAFVTQAN
                     VVGVYGKGFDVLK"
     gene            complement(6285..>7886)
                     /locus_tag="PLN75_06935"
     CDS             complement(6285..>7886)
                     /locus_tag="PLN75_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRG03887.1"
                     /translation="TGAIMAVPGHDSRDYAFAKHFNLPIIPLIEGADISEESFDAKEG
                     IMINSGFLNGLTVPEAIAKAKEYIEEEGIGKVKVNYRLRDAIFSRQRYWGEPFPVYYK
                     DDLPYMLDESKLPLELPEVDKYLPTETGEPPLGRAKNWATDDGYKLELNTMPGFAGSS
                     AYYLRYMDPKNDKALVSTEANEYWQDVDLYIGGTEHATGHLIYSRFWNKFLFDLGLVT
                     KDEPFKKLINQGMIQGRSNFVYRIQGTNTFVSLNLKDQYDVTPIHVDVNIVSNDVLDV
                     EKFRNWNPEYKNAEFILENGKYICGWAVEKMSKSMFNVVNPDDIVEKYGADTLRLYEM
                     FLGPLEQSKPWDTNGIDGVHRFLKRLWSLFFRDNNLSFSNETPTPEELKVLHKTIKKI
                     SSDIETFSYNTSVSAFMICVNELYTLKCNKKSIMEPLTIILAPFAPHIAEELYHLLGN
                     TTSVCDAQWPLHNEEYLKESTVKYPVSFNGKVRFTLELPADMSREDVEKTTLTNEQTQ
                     KYLDGKTPKKVIVVPGKIVNIVVAP"
BASE COUNT         2212 a         1674 c         1705 g         2295 t
ORIGIN      
        1 agccccgttt gccgctcctg ataagtgctg aaaatctgta aatggctccc gggtgatttg
       61 tccaaccttg atttcagatt aaaaataata aaatgcaaaa tggcggattc tgaatccgcc
      121 atttgtgttt tagatgtact tatttctcat tcaatccact cgaatccggt gtaaggcacc
      181 aacgctttcg gaatacgaat tccttcggga gtttggttgt tttcgagcag agctgctact
      241 atccggggca gtgccagggc actcccgtta agtgtgtgac acagttgaat ttttttatct
      301 gcattgcggt agcggcattt caggcggttt gcctggtagt tctcaaaatt cgatacagaa
      361 ctcacttcga gccagcgttg ctgtgctgct gaatatactt caaagtcgaa tgtaagagcg
      421 gaagtaaaac tcatatcgcc gccgcaaagt cgaagaattc tccagggaag ttcaagtttt
      481 tccaccagaa tttgtacata ttccaccatc tcggtaagcg attcgtagga gtgttcggga
      541 gtgtcaatgc ggacaatttc taccttgtca aactgatgaa gacggttcag tccgcgtaca
      601 tccttgccgt atgatccggc ttcgcgccga aaacaggcag aataagccgt atttttaaca
      661 ggcagatctt cttcgttcag aatcacatca cggtaaatat tcgtaacagg aacttcggcg
      721 gttgggatca ggtataaatc gtccaattgt gcgtgataca tttgtccttc cttgtctgga
      781 agctggccgg ttccgtaacc ggaagcggca tttactacat atggaggctg cacttctagg
      841 tatccggcgt cacgtgcact gtccaggaaa aagttaatca aagcgcgttg aagccgggca
      901 ccttttcctt tataaacagg gaaaccggct ccgctgattt ttacacccag ttcaaaatca
      961 atcagatcat atttctttgc taaatcccag tgaggaacag catctttatg cagttcggga
     1021 atagttccgc cgcttttttc caccacgttg tcttcggcgt gttttccttc aggtacgctc
     1081 tcatgaggaa gatttggaat taagaccagt aaatcattca gtttttgttc aattgcttcc
     1141 ttttcttctg tcagacggtt tacgttttct ttcagcgcta ctgttttatc ttttgctgca
     1201 ttagcttcgg attgcttgcc ttgtttgaac aatattccta tttctttgga aagtgcattc
     1261 aactcagcgg cataagtgtc ggcagctgtt tgagtggttt ttcggcgttc atccagttcc
     1321 actatttggt tgatgatttc ctttccgtcg aaatgttttt ttgccaggtg aaggataaca
     1381 tcttcggtat tttctgtgat gtgttttaat gttaacatcc tatttatatt tacaatttta
     1441 caatttacga ttgggaaatg acaaagttac aaaaaaatct tgtcaacaaa tctaacttgc
     1501 ctgaagattt caatattgag aatcaccaaa agtcccccta aagggaaaca acgtacggca
     1561 ttagccaatt agggggcttt tgaaacaaaa aaatctctca caaaattcat tgtgagagat
     1621 ttcttaatac gttttacccg gttgtttagt ttgttgattc aacgggtttg attgaaacga
     1681 aagatttgtt gtctcttttc tttctgaatt ctacggttcc gtcaatcaaa gcaaacagtg
     1741 tgtggtcttt accaattccg atgttcaaac cgggattgtg tactgttccg cgctgacgaa
     1801 ctataatgtt gccggcttta gcgaattgtc ctccgaatat ctttactcca agtcgtttgc
     1861 tttgcgattc acgaccgttg tgtgaactac cttgaccttt cttgtgtgcc attttcttct
     1921 aatttttaat cggttaagct aatatttctt taattgttaa ttcggtgaag tcctgacggt
     1981 gaccattgcg tttgcgatag cctttacgtt ttttcttgtg gaaaacgatt actttttcac
     2041 ctcttacatg cgaaactact tcgcaaacta cttttgcacc atcaacaacg ggagaaccca
     2101 ctgttacggc tccatcattg tcaactaaaa gaactttgtc aaattcaact ttagagcctc
     2161 tttcggcttc gttcatacga tgaacgaaaa gttttttacc ttgttcaacc ttaaattgct
     2221 gacccaggat ttctacgatt gcgtacattc aatttatttt tttgagatta catcggtgag
     2281 gtgagatgac tcgatatttt tgagcagcgc tgctttcttt taccccattc gaattgagcg
     2341 tgcaaatata cgacattatt tgtaaacagg caatattccc cgtaaaatat ttcttacaat
     2401 aattgaatgt acattgaaca ttctgtatgt tgcgaaagtt aaatatttgc ggagtgcact
     2461 gtacatgttt tttttattcg tttttcatgt cattttgtcc gatttgaatc atgcgaaggg
     2521 agagcacttt tatgacaaca ttaccgaaag ttgaaggaaa agtatttcgg tactattttt
     2581 gactttaatt tttctcaaat gccacaaatt atttattttt gtggaatacc gcagaaaaaa
     2641 aagtgagcat tgagaaattc atacaaactt tattacattt ataaaactat atgtcagtga
     2701 aatattcaga tcaattaaat aacgtattca cttatagtct gcaggaagca gaacgccttg
     2761 ggaatgccta tgtgggaccc gaacatcttt ttctcggcct gatacgcaat gcagaaactc
     2821 atgctgcaga attaatgcag gcaatgggtg tagatatcaa cagcgcaaaa caaagaattg
     2881 aggccatggt acgtacggat agcgaaccga ccggtgctga tattccgatg ctgaaaagca
     2941 ccgaaaaaat caaaaaagtg atggaactcg aagcacgctc catgtcagcc gaaacggcag
     3001 ataccgaaca cctgctgctg gctattctga aagataaaaa taatctggct gtggatgtac
     3061 tgagtgccga taacctgact tatgaaaaac tcataaaaca tctcaatcca atgaaacctc
     3121 aggatacgca ggccagcgca ggacttcctc atgaagatga cgaagatgac gaagatcagt
     3181 acggttcaag gaaatcaaaa accaagcagt caggcaacaa accgagcgaa acgcctgcat
     3241 tggatacctt tggaactgat attaccaaag cagctgtgga aaacagactc gacccggtag
     3301 taggaaggga aagggaaatt gagcggattg ctcagattct gagccgtcgg aaaaaaaata
     3361 atcctgtact aattggtgat cccggtgtgg gaaaatcggc tatagtagaa ggtttagcgc
     3421 tgcgcatagt tcagcataaa gtttcgcgcg tgctgtttga taaacgtgtg gtggctcttg
     3481 acatggcttc tatcgttgcc ggaacaaaat accggggtca gtttgaagaa cgtatcaaag
     3541 caatattgaa cgagctgcag aaaaacccca atattattct ttttattgat gaaattcata
     3601 ccattgtggg agctggaggt gcaccgggtt cattggatgc tgccaacatg ctgaaacccg
     3661 ccttatcgcg gggtgaaatc cagtgtgtgg gagctaccac gcttgatgaa tatcgtcaga
     3721 gtattgaaaa ggacggagcg ctggaacgcc gtttccaaaa agtaatggtt gatccgacca
     3781 ctcctgagga aacactcact attctgcaga atatccagga caggtacgaa tatcatcaca
     3841 acgtacgcta tacgcccgaa gccatcaaag cttgtgtacg cctcaccgac agatacatta
     3901 ccgaccggag tttccctgat aaagccatcg atgcactcga cgaggccgga tcaagggtgc
     3961 acatagctac cgtatcggtg ccaaccgaaa tagaacagct tgagaaaagg cttgaagacc
     4021 taaaagctga aaagaacagg gttattgccg aacaaaaata cgaagaagca gtacccatac
     4081 gtgataagga aaagcagacc caatatcagc tcgaagatgc aataaaacgg tgggagaaag
     4141 aatcgcagct gaatccggaa acggttgacg aagaaaaagt ggctgaagta gtggccatga
     4201 tgtcaggcat tcccgtgcag cgtattgctc aggccgaagg acttaaactg cggcagatgg
     4261 gagaggtgct caaatcgagg gttatcggcc agaatgacgc agttgataaa attacgaaag
     4321 ccattcagcg caaccgcatc gggctgaaag atccgaacaa accgattggc tcatttcttt
     4381 tccttggtcc gaccggagtt gggaaaactc atctggcgag ggtgctggca gattttatgt
     4441 ttgacagtac cgatgcgttg attcgggtgg atatgagtga atacatggaa aaattcagcg
     4501 tttcacgttt aatcggtgca cctccgggat acgtaggata cgaagaaggc ggacaattga
     4561 ccgagaaagt acgccggaaa ccgtattcca ttgttctgct cgatgaaata gagaaagcgc
     4621 accccgatgt tttcaatatc atgcttcagg tgatggatga aggacgactc accgacagtc
     4681 ttggacgccg aattgatttc aaaaatacca ttattataat gacttctaat gtcggtaccc
     4741 gccaactgaa agattttggt cgcggagtag gtttcaacat ggacgaagat atgaccatcg
     4801 attcagacta ctctcgcgga gttattcaga aagcactgaa taagactttt gctcccgaat
     4861 ttctaaatcg cgtggatgat attgtgatgt tcgatcagtt aaaccgcgaa agcctgaaat
     4921 ccattatcga cctggaactg aaaggtatat ttaaacgtgt gaccgatctg ggttacaaac
     4981 tggaactctc acccgaggcc aaagatttta ttgccgataa agggtatgat gttcagtacg
     5041 gtgcccgtcc gctgaaacgc gctatccagc gttatctgga agacgaactg gccgatgtgg
     5101 tgcttgcagg agaggccgcc gaaggagata ccattctgat ggaactcaat gaagcaaaag
     5161 acggcgtgaa agccagggtt gtgaaacaaa atccgacaat tacggaaaca gaatagcgat
     5221 tttttctttc tattaaataa aaaaaaggag tgatataaaa tcgctccttt tattcttaaa
     5281 gtcccctttt gggggattta gggggcttct acttcaatac atcaaatcct ttgccgtaaa
     5341 cacccactac attggcttga gtaacgaatg catcctgatc aatgcttttt acaagtctga
     5401 aaatggaggt ggattcggtt ttgcgggcaa gaacaataat gattttttgc gatttctttg
     5461 aataccagcc cattccgtca attatagtac atccccgttt tatctccgaa ttgatatgac
     5521 tggccacttc atcgtatttg gaggtaaaga taaaaaactg aaccgactgc cgcataccac
     5581 ttatcattac gtcaacagcg taatacatca ctgccattac gatcagaccg atggcaattt
     5641 tttcaacact tctgaacagg aaaaatgaag aggctacgat taaaatatct acgagcagca
     5701 acacccgccc catgcttaca tggcggtatt ttgttaccac agcacctatt atgtcagttc
     5761 cgccggtgct cccgtttact gaatacacaa gacctaatcc cgcaccgcaa aacatggcgc
     5821 caatcatgga tgaaagcagt gcgtcggcat gtatgattgg tttggtaatg taaaattcac
     5881 cgtatctcag cagaatagta agtactgcta cactgactac ggttttgata acgaaatttt
     5941 taccgacaat tttccaggca aaaatcagca acaccgcatt tatcgaaacg taagataccc
     6001 aaagcggaat ttcagttgcc gattttatca aaactgttac acccgccagg ccacctgtta
     6061 cgattttatt tggtaacaga aatccggtca gaccaacagc atataatatc aatccggcga
     6121 aaatcataat gtaatctttc cagtagaaag attttaaata atcttttttt gaattcataa
     6181 tagccctcat gtaccctgta gggtgatttt agattgaatg aattgaaaaa tttctatatt
     6241 aaataaagtt tttgagttcc ggcacaagtt ctcttttcag gagatcaggg ggcaactaca
     6301 atatttacaa tttttccggg aactacaatt actttcttcg gcgtttttcc atccaggtat
     6361 ttttgagtct gttcgttggt cagggtagtt ttttctacat cctccctgct catgtcggca
     6421 ggcaattcga gcgtgaaacg tactttgccg ttgaacgaaa cgggatattt cactgtactt
     6481 tccttcaggt actcttcgtt gtgcaggggc cactgagcat cacataccga agtggtattt
     6541 ccgagtaaat gatacaattc ttcggcaatg tggggtgcaa aaggtgccag aatgatggtg
     6601 agcggttcca taattgactt tttattgcat ttcagcgtgt aaagttcatt tacacaaatc
     6661 ataaatgccg aaacagacgt attgtaagag aacgtttcga tgtcgctgct gatttttttg
     6721 atggttttgt gtaatacttt cagttcttcg ggagttggag tctcgttgct gaagctcagg
     6781 ttgttatctc tgaagaataa actccagagc cttttcagaa aacggtgaac cccgtcaatt
     6841 ccgttggtgt cccagggttt ggattgctcc agcgggccca gaaacatttc gtagagccgc
     6901 aaggtgtctg ctccgtattt ttctactatg tcatcgggat ttactacgtt gaacatcgat
     6961 tttgacattt tctcaaccgc ccatccgcaa atgtatttac cgttttcaag aataaattcg
     7021 gcgttcttgt attccgggtt ccagttacgg aatttttcaa catccagtac atcgttcgat
     7081 acgatgttta catccacgtg aatgggcgtt acatcgtatt gatctttcag atttaatgaa
     7141 acaaaagtat tggtaccctg tattcggtac acgaaattgc ttcttccctg tatcattccc
     7201 tgattaatca gttttttgaa tggctcgtct ttagtcacca atccaaggtc aaaaaggaat
     7261 ttattccaga agcgactgta aatcaggtgg ccggtagcat gctctgttcc gccgatgtac
     7321 agatccacat cctgccagta ctcgtttgcc tccgtgctga ccagcgcttt gtcgtttttc
     7381 ggatccatgt atcggagata ataagccgaa gatcctgcaa aaccgggcat tgtgttcagt
     7441 tcaagtttgt atccgtcatc cgttgcccag ttttttgctc gtcccagcgg cggttcgccc
     7501 gtttcggtgg gcaggtattt atccacttcg ggaagttcga gaggtaactt gctctcatcg
     7561 agcatataag gcaaatcatc cttgtaatat accggaaacg gttcacccca ataccgctga
     7621 cgactgaaaa tggcatcacg gagacggtaa ttgaccttca cttttcctat tccttcttct
     7681 tcaatgtatt cttttgcttt cgcaatggct tcaggaacag taagaccgtt caggaaacct
     7741 gaatttatca ttatcccttc tttggcatca aaactttctt cacttatatc agctccctcg
     7801 ataagcggaa ttatcggcag attgaaatgt tttgcaaaag cgtagtcacg gctatcgtga
     7861 cccggaacag ccataatggc accggt
//
LOCUS       DAORKP010000110         7692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1561_length_7692_cov_6.903496, whole genome shotgun sequence.
ACCESSION   DAORKP010000110 DAORKP010000000
VERSION     DAORKP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_7692_cov_6.903496"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..2323
                     /locus_tag="PLN75_06940"
     CDS             74..2323
                     /locus_tag="PLN75_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG03888.1"
                     /translation="MRKESKYNKSLIDLAEVLLEDNNTLSIRVSGSSMYPSLKEGDIC
                     YEQKCPVEDLKKGDIIACRKNNFLIMHRLKRLSKRPDGKYVIEMRGDNVLHYDPPFDE
                     EEFVAKLLYFKRDGKIHQVDSPSMKFKKFIDDYFHPVSCRFNKGLLFTKYAFSVIRKQ
                     NSSLFTNLGQLIRGNQSQFRSNALIAVLKGIVPIGMLVSIKFLIDFLTHSSFGQPEQK
                     LLFFALLGVTALLFLAGGVLTQIGNYTAEKMSQSVTRRVYDELHQKHIRLHLTDFENS
                     AQLDRMHRAVQEASYRPVRILNSALGLIKTCSAGILLLALFVTIRWYLVIILLVAVLP
                     EAVFRMAYSRKLYRMKEKQAPDEREKSYYNRVITGFPFAKEIRLFDFPDYFIRLFNRK
                     QDLLFDEKLKLSRYELRTSILAQVFAVLLIFSTLGIVSYLSIAGMMTIGTVVLFFFAF
                     QRGYSILNEFFRSVTSLVEDNSFLQDYLSFLQIPDVQLNKVISPKPFRLENEIRFDNV
                     SFRYSESDREALRNVNIRIPAGKTVALVGENGAGKSTLVKLLCGFYRPTGGEILLDGV
                     PAGEIGQVELLRGITAVFQDFALYQVSAADNILLGNYRKPRNMTQMKEAAAEAGIAET
                     LEKLPQGYDTRLGHLFGESEELSIGQWQKMALARAFYRDAPLLLMDEPSSALDANAER
                     QLIESLKALARNKTTLIVSHRLSTVQWADIIYLLHRGEVAETGTHEELIQLRGRYYRL
                     FSKNENGVK"
     gene            2656..3252
                     /locus_tag="PLN75_06945"
     CDS             2656..3252
                     /locus_tag="PLN75_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRG03889.1"
                     /translation="MNIFENSSTQPFQQPDPDNATGNSGTPVPTADGRHWLAAYVKMH
                     HERKTRDMLNAMGIENFLPVQEEVRRWSDRLKKMERILIPMMIFVRVNAAEQRQVIQL
                     PAVIRYLVLRGEHRPAIIPDYQMNHFRFLLDHAENTVTIEQELLVPGISVRVVKGPLQ
                     GLEGILLTVEGNSRIAVRIEQLGYATVEMHMGMVEINS"
     gene            complement(3938..4708)
                     /locus_tag="PLN75_06950"
     CDS             complement(3938..4708)
                     /locus_tag="PLN75_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03890.1"
                     /translation="MLKNNKYGRIHKMETPTLIKGVNAIVKKYDTVDLDLKTWCDQLL
                     NQESELAELTEINLTHPAMKELKKQRKARKKLLGVVNIHYSALERAQVDSLTTQQEVV
                     IPFLKQYVRNTITNNNKKMEEIVIQMLRTIEENEPLRMALQTVGLKVYFDELKMIQQN
                     ITLCKTKIVAFKSETPKMRTAAIKKSAVNALSNLIQSIELGKIRYPEKDYMPLTNELE
                     AFLSEYQMLDKTRRTISKKIAENKKTVASSPTTDATAS"
     gene            complement(5197..>5398)
                     /locus_tag="PLN75_06955"
     CDS             complement(5197..>5398)
                     /locus_tag="PLN75_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03891.1"
                     /translation="RKKGLIELRLPLLLRIKGVIRPRLTSLLRIKGVIRPRLTQLLRI
                     KMSDHTATNPIAPDKKNGGSSP"
     assembly_gap    5399..5498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6380..7690
                     /locus_tag="PLN75_06960"
     CDS             6380..7690
                     /locus_tag="PLN75_06960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRG03892.1"
                     /translation="MNITIVGTGYVGLVSGTCFAELGSNVTCVDVNEEKIANLKQGIV
                     PIYEPGLDSMISKNVNAGRLHFTTRLHEVIEDTEIVFIAVGTPSGEDGSADLKYVLDV
                     ARSIGRSINKYLVVVVKSTVPVGTSLKVKQAIREELGKRNADVEFDVASNPEFLKEGD
                     AIDDFMKPDRVVVGVETEKARKWMTQLYQPMLLNNFRVIFMDIPSAEMTKYAANAMLA
                     TRISFMNDIANLCELVGADVNMVRRGIGSDSRIGNKFLYPGCGYGGSCFPKDVKALIK
                     TASLHNYDLKVLNAVEEVNDKQKNILFEKFHTLFHGDVFGKKVAVWGLAFKPGTDDMR
                     EAPSLVLIDKLLEAGCEVAVYDPVAMEESKRRIGDKVNYAKDMYDAVLDAQVIFHVTE
                     WKEFRMPNWEVIKRSMKPQPVMIDGRNVFDAETLEGFTYLSIGR"
BASE COUNT         2140 a         1611 c         1647 g         2194 t
ORIGIN      
        1 cgcaacaaac cgcttagagc ggttggttgc tcgtgctaag cggttggttg ctcgtactga
       61 aaaaattaaa tttatgcgta aagaatccaa atacaacaaa tccctgattg acctggcaga
      121 ggtgcttctg gaggataata atacgctgag catacgtgtg agcggtagca gcatgtatcc
      181 ttcgctgaag gaaggagata tctgttatga gcaaaaatgc cctgtcgaag atttaaaaaa
      241 aggtgatatc attgcctgca gaaagaataa ttttcttatt atgcatcgcc tcaaacgatt
      301 gtcaaaaagg cccgacggaa aatatgtcat tgaaatgcgg ggagacaatg tgttgcatta
      361 cgacccgcca ttcgatgaag aagagtttgt agcaaaactc ctttatttca aacgtgacgg
      421 aaagattcat caggtcgaca gtccatccat gaaattcaaa aaattcattg atgattattt
      481 tcacccggtt tcatgccggt tcaacaaagg cttgttattt acaaaatatg ctttttctgt
      541 gatcagaaag caaaacagtt ctttgtttac caatctcggt cagttgattc ggggtaatca
      601 gagtcagttt cgctccaatg cgctcattgc ggtactcaaa ggcattgttc cgatcggcat
      661 gctggtaagc attaaatttc tcatcgactt tctgacccat tcttcgttcg ggcagccgga
      721 gcagaaatta ctctttttcg ccttgctcgg tgtcactgcg ttgctctttc tggccggggg
      781 agtgctcacg cagattggaa actataccgc cgagaagatg tcccaatccg ttacccgccg
      841 ggtgtacgac gaacttcatc agaagcacat ccgcctgcac ctcaccgact ttgagaattc
      901 cgctcaactg gaccgcatgc atcgtgctgt tcaggaagct tcgtaccggc ctgtccgaat
      961 tctcaactca gccctcgggc ttatcaaaac atgcagcgca ggaatactcc tgctggcact
     1021 ctttgtaacc atacggtggt atctggtcat tattttgctt gtggcggtac tacctgaagc
     1081 cgttttcaga atggcataca gtcgcaaact gtaccggatg aaggaaaaac aggctcccga
     1141 cgaacgggaa aaatcgtatt acaacagggt aataaccggt tttccctttg ccaaggaaat
     1201 acgccttttc gactttccgg attatttcat ccgacttttc aaccggaaac aggacctgct
     1261 tttcgacgag aaactgaagc tcagccggta tgaactccgt acctccatcc tggcacaggt
     1321 attcgcagta ttgcttattt tttccaccct cggcatcgtg tcgtacctca gcattgcagg
     1381 catgatgacc atcggaacgg tggtgctttt tttctttgcc tttcagcggg gatactccat
     1441 cctgaacgag tttttccgtt cggttacctc gcttgtggaa gacaattcgt tccttcagga
     1501 ttacctttct tttctgcaaa tacctgacgt acaattaaat aaagtgatca gcccgaaacc
     1561 tttccgcctc gaaaatgaaa tccgattcga caatgtaagt ttccggtaca gcgaaagcga
     1621 tcgcgaagca ctccgaaacg tgaacatacg cattccggcc ggtaaaaccg tggcgctggt
     1681 gggtgaaaac ggtgccggga aatccacatt ggtgaagttg ctctgcggat tctaccgccc
     1741 caccggaggc gaaatcctgc tcgacggtgt tcctgcaggt gaaatagggc aggtggaact
     1801 gctgaggggt atcactgctg tatttcagga ctttgcgctc tatcaggtat cagcagccga
     1861 caatatcctc ctgggtaatt accggaaacc gcgtaacatg actcagatga aggaagctgc
     1921 tgccgaagcc ggcattgcag agacactgga gaaacttccg cagggttacg acacacgcct
     1981 tggacacctt tttggcgaaa gtgaggaact cagcatcgga cagtggcaga aaatggcgct
     2041 ggcacgggct ttctaccgcg atgcaccgtt gctgcttatg gacgaacctt ccagtgccct
     2101 tgatgcaaac gctgaacgcc agctgattga gagcctcaaa gcccttgccc ggaataagac
     2161 cacccttata gtcagtcacc ggctgtccac cgtgcagtgg gccgatatca tctacctgct
     2221 tcacaggggt gaagtggccg aaaccggcac tcacgaagaa ctgatacaac tccgcggaag
     2281 gtattaccgg ttattcagca aaaacgaaaa cggagttaag tgaaacaagt tttaaagttc
     2341 tgaattcgga gccagcctgg ttttgaaagg ttttaaatta gtttaacgtg atttcgatat
     2401 aagaaaaact acttgtttaa tcgtaataat ttaaatatca ataagttaat gaggtttatt
     2461 tttctcttca tctatttcga ctaaggtaga taatcaagat aaggtttcat ttttcttata
     2521 aaaaccttat tttccaaaag tatcttagta gaatggaaaa aattagcatt tctgtcctta
     2581 ttaaccttat attcagaaga ataaatttat ttataaatct aactcacgtt agttaattta
     2641 caaattttta ttgaaatgaa cattttcgaa aattcatcca cacagccttt tcagcagccc
     2701 gatcctgaca atgcgaccgg caactccggc acgcctgtgc caacagccga cggccggcac
     2761 tggctggcag cttatgtaaa gatgcatcac gaacggaaaa cgcgcgatat gctgaacgca
     2821 atgggcatcg aaaacttttt gcccgtacag gaggaagtgc gccggtggag cgaccgcctg
     2881 aaaaaaatgg agcgtatcct cattccgatg atgatattcg tgcgggtgaa tgcagccgaa
     2941 cagcggcagg tgattcagct gcctgccgta atccgctacc tggtgcttcg aggtgagcac
     3001 cggccagcca ttatccccga ttaccagatg aaccatttcc gctttctgct cgatcatgcg
     3061 gagaatacag tcaccattga gcaggaattg cttgttcctg gcatttcggt tcgcgtggtc
     3121 aaagggccgc tacaggggct cgaaggcatc ctcctgactg tggaaggcaa ctcacgcatc
     3181 gcagtgcgga tagaacagct cggatatgct accgtggaaa tgcacatggg aatggttgag
     3241 ataaactctt aaatcccgca tgaacgggat gattttatta agataattga ttttagtaaa
     3301 ggtaatatgt gtagttcata tatacgttat tgatcatttt tttaatagaa ttaaacatgg
     3361 tggctttgat taataagaat aaggttatat ttcctgtatt tgcatttcta ctaaggttga
     3421 agatgaaaaa taaagttatt gataaatctg aaaaacctta tttccaagta tatatttacc
     3481 ttagtagaaa atttatgtat atgattttaa ccttattagt aatatgtatt tttagtaacc
     3541 gccattttta gtataagtaa actgtgtcgt attttgttaa tatacgaata gatatatttc
     3601 ttttttatcg aatggttaag tgctaaaaat cgccgcatga aggggtgaac ttttttaccg
     3661 gtgggaactg aaagacagcg gtatttcggt tataattcaa aaaaaagtgc aggaattgca
     3721 gtgattacac cgggaaatat tgttaaatga cccaaaaacg ctattatgtg ttaaataaat
     3781 taaaaactaa atgttaaaca tgattgcatg aaaatcatat atcaaaaaat agtatacttt
     3841 tgcacgtgta tcaatgtttg tatcaatttt ttctgagagg aaaaaaaaga accaactccg
     3901 caaacaggca tacgaagctg gttcccagca aaaacttcta agaagcagtc gcgtcagttg
     3961 taggagacga agcgactgtt tttttatttt ctgctatttt ttttgaaatt gttcttcgcg
     4021 ttttatccag catctggtat tcactcagaa acgcttccag ttcattggtc agtggcatat
     4081 agtccttttc gggatatctg attttaccca gttctatgga ttgtatcaaa ttgctaagtg
     4141 cgttgaccgc cgattttttg atagctgctg tccgcatctt cggagtctcc gatttgaatg
     4201 caactatctt tgttttacac aatgtaatat tttgctgaat cattttcagc tcgtcgaaat
     4261 ataccttcag ccccactgtc tgcagtgcca tccgcagcgg ttcattctcc tcaatagtac
     4321 gaagcatttg aataacaatc tcctccatct ttttattgtt gttggtgatt gtgtttcgca
     4381 catattgttt cagaaaaggt atcactactt cctgctgcgt tgtcagcgaa tcgacctgag
     4441 cacgttcgag tgccgaataa tgaatgttaa ccacgccgag aagctttttt cgggctttcc
     4501 gttgtttttt caactctttc attgccggat gcgtcagatt gatttctgta agttccgcga
     4561 gttcagattc ctgattcagt aattgatcac accatgtttt taggtccaaa tccaccgtgt
     4621 cgtatttttt cacgatggca tttacacctt tgataagcgt tggggtctcc atcttgtgga
     4681 ttctcccgta tttattattt ttcaacataa taaaataaaa tttataaatt aatattataa
     4741 ttattacgct gcgctttcat caacggcatt actgaaacag cctgtttata tttcatcgtg
     4801 tcaaagatcg aattacgcaa gcgtaaccct gcgaaaatgc aaaaaatcca ttcttcacca
     4861 atttcgcact gcaatgttag tgaatatttt ttaaataaaa cgattttttt ttataaaata
     4921 tttcgaaaaa ttttcaacaa atttctaaca ataagttcaa tccattgaat atcagtaata
     4981 taaattaatt gaatgttgaa aaagttttcc aaaaaataaa tatttatttt cagaaaatac
     5041 gatttaacag tcgaaaaagg cggcttttac ctacaaaaaa taaatccggc ctctccccgc
     5101 gagaaaaaat ccggcctctc cccgcgacaa tttccatccc gaagagattt tgcacgcccc
     5161 tctccaaaag agaggggagg aagattcttt ttctaactag gggctgctgc ccccattttt
     5221 tttatccgga gcaattgggt tagtcgcggt atgatcactc atttttatcc ggagcaattg
     5281 ggttagtcgc ggtctgatca ccccttttat ccggagcaat gaggttagtc gcggtctgat
     5341 cacccctttt atccggagca ataagggtag tcgcagttcg atcaaccctt ttttccggnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cggagcaata agggtagtcg
     5521 cagttcgatc aacccttttt tccgggattt ttgtgcctgt tttgatgagt gaagttgcat
     5581 tatcttttgt ctaatcacct gataattaat gcagaataac gattttatgc aacttgttta
     5641 tcgccgttat ctatcggaca atttcataca cagaagcggt accaatacgc cttgccgttt
     5701 ttttttcgaa tacagagaca cctgtcatta cagataatta tcttttttta actcagactg
     5761 aggaattgaa ttaacgggta aaacatttaa taatcaaaac attaagtttt aagtttcaag
     5821 ccagctgtaa ttttttaata tatatcagaa ttgtattaaa cagtgctttt taaatacaaa
     5881 taaaaatggt aactttgcat gaatttttgc tattccggaa tgctgttcat ttttgttgat
     5941 aaacgcgatt ccaatgtaaa atcatggcat gctgattttt tgctaacccg ccatatttta
     6001 agtagtttac agaacattca gtatgcgttt ttttcagaga catgatgtat tcatttcggt
     6061 cctctgcaag cgcaggaatc tggtagcaga tacgatagcg tattcacata cgatgtactg
     6121 catccggacg gtcggtttta tttctttttt tctatctgcc aatgtgacag gtagtcacga
     6181 agtgtgcccg gaaagttcgt ctgtcctttt ttagtgattg ggtgactccg attcacccgc
     6241 tcacttacgc gcaaatttgc ataaaaatgc cttatccgta taaatccgtc aaatccgcat
     6301 catccgcgta ctaaataaaa cccaaaagcc tctgcccggc cgctatcggt atcagggcag
     6361 ccaaaatacg aaatagaata tgaatataac aattgtaggt accggatacg taggattggt
     6421 ctccggaaca tgttttgcag agttgggctc caatgttaca tgcgtggatg tgaacgaaga
     6481 aaagattgcc aatcttaagc agggcattgt acccatttac gaacccggac tggactctat
     6541 gatcagtaaa aacgtcaatg ccggacggct tcatttcact acgcggctac atgaggtgat
     6601 tgaagacact gagattgtat ttatagctgt aggtacacca tccggtgagg acggaagcgc
     6661 cgacctgaaa tatgtgctgg acgtggcacg cagtataggc cgttccatca acaaatacct
     6721 ggtggtagtg gtgaaaagta ctgtaccggt gggcacttcg ctgaaagtga aacaggccat
     6781 tcgcgaagag ctggggaaaa gaaatgccga cgtggaattt gatgtggctt caaatcctga
     6841 atttctgaaa gagggcgacg ccatcgatga ctttatgaaa cccgaccgcg tggtggttgg
     6901 cgtggaaacg gaaaaagccc gaaaatggat gacccaactg taccaaccca tgttgctgaa
     6961 taatttcagg gtgatattta tggacattcc atcggcagaa atgaccaagt atgcagccaa
     7021 tgccatgctc gccacccgta tcagttttat gaatgacatt gccaatctgt gtgaactcgt
     7081 tggcgccgat gtcaacatgg ttcgccgcgg tatcgggagt gacagccgga ttggcaacaa
     7141 attcctatac ccgggatgcg gttatggcgg gtcctgtttc ccgaaagacg tgaaagcgtt
     7201 gattaaaacc gcctctctcc acaattacga tttgaaagtg ctgaatgcgg tggaagaggt
     7261 gaatgataaa cagaaaaaca ttctttttga aaaattccat accttgttcc acggagatgt
     7321 tttcggtaaa aaagtggctg tatggggcct ggctttcaaa cccggcaccg atgatatgcg
     7381 cgaagctccg tcgctggtgc ttatcgacaa attgctcgaa gccggatgcg aggtggcggt
     7441 ttacgatcct gtggcgatgg aagagagtaa aagaagaatc ggcgataaag taaactatgc
     7501 caaagacatg tacgatgccg tactcgatgc gcaggtaatc ttccacgtga ccgaatggaa
     7561 agaattccgc atgcccaact gggaagtcat caaacgctcc atgaaacccc aaccggtcat
     7621 gattgacgga agaaatgttt ttgatgcaga aacgttggaa ggatttacct atttaagcat
     7681 cgggcgataa ta
//
LOCUS       DAORKP010000111         7655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1577_length_7655_cov_8.962237, whole genome shotgun sequence.
ACCESSION   DAORKP010000111 DAORKP010000000
VERSION     DAORKP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_7655_cov_8.962237"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2209)
                     /locus_tag="PLN75_06965"
     CDS             complement(197..2209)
                     /locus_tag="PLN75_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRG03893.1"
                     /translation="MEQNQEKPVNLPENAYRELKEGEEYKPVMPAGSSVAEVTPWSVG
                     MGLLMAVIFSAAAAYSGLKIGQVFEAAIPISIIAVGASAAFRKKGALGQNVIIQSIGA
                     SSGVIVAGAVFTIPGLYILQAKYPEIQVDFWQIFFSSLFGGFLGILFLIPFRKYFVKD
                     MHGKLPFPEATATTEILMTGEKGGNQAKLLVTSGLIGGLFDFSFSAFRLWSEEISTRI
                     VPVGAILAEKFKMVLKFNVSALIFSFGYLVGLRYALIITVGSLLSWLVLIPLVNEFGM
                     ISATLSGGVNPFAAMSAEQIFAAYVRPIGIGAIAMAGIIGIIKSSGVIGSAFKLAVGG
                     KTKGHAEEKTERTQRDIKMTFVMLFLFLTLVAVFIFLLVGVKVTLLQAVIALLTIAVI
                     SFLFTTVAANAIAIVGTNPVSGMTLMTLILSSVILVAVGLKGWQGMVSGLIIGGVVCT
                     ALSMAGGFVTDLKIGYWLGTTPAKQESFKFLGTLVSAATVGAVIYILNEAYGFVATPE
                     HTNPMVAPQANAMAAIIEPLMSGTGVSWILLGVGAVISILINWLGISPLAFALGMFIP
                     LPLNTPLVVGGLLNQWFNRSTKNAKLNNARHQRGILIASGFIAGAALFGVVGALVIFI
                     TGRGDALDLKVWTDPSGVGAQVTALVAFVALIAFFVWESFKAKEED"
     gene            complement(2222..4216)
                     /gene="pulA"
                     /locus_tag="PLN75_06970"
     CDS             complement(2222..4216)
                     /gene="pulA"
                     /locus_tag="PLN75_06970"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRG03894.1"
                     /translation="MKKTWIILAISGILISCTGSGKKFKSYEEFPVYEGNDLELTYSP
                     SGSKFRVWSPAADEVKLLLFDNGLDGSAYEMKDMKRSKSGTWIANVKGDLKGKYYTFQ
                     VKMGEKWLNETPGMWVKAVGINGKRAAIIDINETNPDGWENDKHVLPEHVTDAVLYEV
                     HMRDFSVSPNSGIKNKGKFLAFTEKGTKNTAGQSTGIDHLKELGITHVHLLPSYDYAS
                     VDETKLKENKYNWGYDPLNYNVPEGSYSTDPANPVSRIKEFKQMVQSLHSEGIGVVMD
                     VVYNHTYVGEESHLNLLVPGYFYRFNADSTWSNASGCGNETASDRAMMRKFIVESVVY
                     WATEYHVDGFRFDLMGIHDIETMNAIRAALDKVNPKIILYGEGWTAAASPLGEEKRAV
                     KKNVSKLNGIAVFSDDIRDALKGNWMNEFPGFVSGRDSLENAVKFGIVASTPYPGIDT
                     TKLVHSKAPYATQPTQVINYVSCHDDMCLVDKLRKRKPAGATEAEIQKFNKLAQTVVF
                     TSQGIPFIYAGEEIYRDKKGVNNTYESPDSINQINWDNKTTYNDIFQYYQGLIALRKA
                     HPAFRMTDFEMMKKHLKFIELNVPNVIAFTLSDNANGDSWKDILVILNGNRKNVKVEI
                     PEGEWNVVCHDGKISPSIPLMTVKNPLFTLGASSASIMYK"
     gene            complement(4249..6312)
                     /locus_tag="PLN75_06975"
     CDS             complement(4249..6312)
                     /locus_tag="PLN75_06975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRG03895.1"
                     /translation="MTALTITAGVFAANPLLQPYKTPYQTVPFDEIKTEHFKPAFLEA
                     MKQNKAEIEALVNNPKPATFENTIVALDKAGRLLARVSSPFYNLLGAETSDELQAVAQ
                     ELSPLMSEHSNEILLNEKLFERIKSVYDQKDKLKLNAEQKTLLKDTYDGFANNGANLS
                     DADKARYRELTKELSMLTLTYGQNVLKETNKYEMLIKNPETLKGLPEDVMEMLAENAR
                     KAGKEGWLINLKTTSYAPVMKYADNRDLRRELYLASSTKCMIGGDYDNRENLRKIANL
                     RLQIANLLGHKTYADHVLQRRMAENTDNVYELLNKLLDAYKPTAEKEYADVQAYADRN
                     GAYFKIQPWDWSYYSEKLKAEKFNLNDEMLKPYFELERVKKGIFWLANKLYGLNFKEN
                     PNIPVYHSEVKAYDVTDSKGKFVAVLYTDYHPRDGKRAGAWMSSTKDQWMDGKKDSRP
                     HIINVMNFTRPTSTKPALLTFDEVTTMLHEFGHALHGMLSKTTYQSLSGTSVYRDFVE
                     LPSQIMENWGVEKEFLDQFAVHYQTGEKMPAELIQKIKDADNYNVGTFTLRQLSFGLL
                     DMKWHTIDKPFEGDVIAFEKEAMAPAQVIPVVENTGMSPTFSHIFSGGYAAGYYSYKW
                     AEVLDADAFSVFLKNGIFDKKTADSFRVNILEKGGSEHPMVLYKRFRGQEPDIKALLQ
                     RHGIK"
     gene            6576..7226
                     /locus_tag="PLN75_06980"
     CDS             6576..7226
                     /locus_tag="PLN75_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRG03896.1"
                     /translation="MKKLEIPSIKALNDVGADSAGDLLEESGYKSVIEILNWPDQYAY
                     KPITYFFIGRSADAVFLKFSVNGTMLKAVYTEDHSPVHEDSCVEFFCMPEGADKYTNF
                     EFNCIGTCSASSRKGRNEDVIPFSKEEMATIERYASIGKKPFKEMEGMFEWELTVKIP
                     LKLMNLDPENLPEKIRGNLYKCADDTDSVHFVSWAPIKTENPDFHRPEFFGEMYFK"
     gene            7372..>7655
                     /locus_tag="PLN75_06985"
     CDS             7372..>7655
                     /locus_tag="PLN75_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRG03897.1"
                     /translation="MKKTFSTALFITAFAAAGSAQNQKPNVILILADDLGYSDIEPYG
                     QEKIKTPNLNRMAENGMQFMQFYCGTSVSAPSRASLMTGLHTGHTHVRGN"
BASE COUNT         2034 a         1730 c         1634 g         2257 t
ORIGIN      
        1 taaattatta aaatattaga tttctcactt cgttcgaaat gacattcagt attttacctt
       61 tcagaacaat ctccacaaac cactgtcgtc cggagcaaag cgaagaaact aaaaaatttt
      121 atcggacaac aataaaaaaa tctaccaatc tgctgattga tagatttttt tagtatccga
      181 aattaatccg ttttttctaa tcctcttctt tggccttaaa tgattcccac acaaaaaatg
      241 caatcagtgc aacgaaagcc actagtgcag ttacctgagc tcctactccg gagggatctg
      301 tccatacttt cagatccaaa gcatcacccc ggccggtaat gaaaataacg agagcgccta
      361 caactccaaa taaagccgcg ccggcaataa atccggatgc tatcagaatt ccgcgctgat
      421 gacgggcgtt gttgagtttc gcattttttg tacttctgtt aaaccattga ttcagaaggc
      481 cgcccacaac cagcggagta ttgagcggta gcggtataaa cattcccagg gcaaatgcaa
      541 gtggtgaaat tcccaaccag ttaatcaaaa tggaaataac agctcccacg cccaacagaa
      601 tccagctgac tccggttccg gacatgagcg gttcgataat ggcagccatg gcgttggcct
      661 ggggggctac cattggattg gtgtgctccg gagtggctac aaatccgtaa gcttcgttca
      721 gaatgtaaat gactgctccc acagtagctg cagaaaccag cgtcccgagg aatttgaatg
      781 attcctgctt agccggagtg gttccaagcc agtaacctat cttcaggtcg gtgacaaaac
      841 ctcccgccat ggataatgct gtgcaaacca caccaccgat tatcagaccg ctgaccatcc
      901 cctgccagcc tttcaaacct acggcaacga ggatcacgga tgataaaatc agcgtcatca
      961 gcgtcattcc cgaaaccgga ttagtaccta caatggcaat ggcattggct gctacggtag
     1021 tgaaaaggaa cgaaatgaca gcgatagtca gcaaagcaat cactgcctga agaagagtga
     1081 ctttaacacc cacaaggaga aaaataaata cggctaccaa cgtcagaaat aagaagagca
     1141 tcacaaaagt cattttaata tcacgctgtg tgcgttctgt tttttcttcg gcgtgtcctt
     1201 tggttttgcc acccacggcc agtttgaaag cgctgccgat tactccggat gatttgataa
     1261 taccgataat tcccgccata gctatggcac cgataccaat agggcgaaca tacgctgcaa
     1321 aaatctgttc ggcggacata gctgcaaacg gattgacacc gccgctcagg gtggctgaaa
     1381 tcattccgaa ttcgtttacc aacggaatta aaaccagcca cgaaagcaac gaaccaaccg
     1441 taatgataag tgcataccga agccccacga ggtatccgaa actaaaaatc agtgcactga
     1501 cattaaattt cagcaccatt ttgaattttt ctgccagtat ggctcccaca ggaacaattc
     1561 tggttgaaat ctcttcgctc caaagacgaa aagctgaaaa gctgaaatca aaaagtcctc
     1621 cgattaaacc gctggttacc agcaattttg cctgatttcc acctttttca ccggtcataa
     1681 gtatttcggt ggtagctgtc gcttccggaa aaggcaattt gccgtgcata tcttttacaa
     1741 aatatttccg gaaaggaatc agaaacaaga ttccgagaaa tccgccaaaa agggatgaga
     1801 aaaatatctg ccagaaatcc acctgtattt cggggtattt tgcctgtaaa atatacagtc
     1861 cgggaatggt aaatacagct cctgcaacaa ttactcccga agatgccccg attgactgaa
     1921 taatcacatt ttgccccagt gctccttttt tccggaatgc tgccgaagct cctactgcaa
     1981 tgatggagat aggtatcgca gcttcgaata cctgccctat tttaaggccg gaataggcag
     2041 ctgcagccga gaaaataaca gccatgagca agcccattcc taccgaccac ggtgtaactt
     2101 cagctactga cgagccggcc ggcatcacag gtttgtactc ttcgccctct ttcagttcgc
     2161 gataggcgtt ttccggtaag tttaccggtt tttcttgatt ttgttccata aatagctgac
     2221 attatttata cattatactt gccgaggatg ctccgagagt aaacaatgga tttttcacag
     2281 tcatgagagg aattgacgga gaaatttttc cgtcgtgaca aaccacattc cattcgcctt
     2341 ccggaatttc cacttttaca tttttccggt ttccgttaag aattaccaga atgtctttcc
     2401 aactatcacc gtttgcatta tcagacagtg tgaatgcaat cacgttgggc acattcagtt
     2461 caatgaattt cagatgcttt ttcatcatct caaaatcagt cattcggaag gccggatggg
     2521 ctttgcgcaa agcaataaga ccctgataat attggaaaat atcgttgtaa gtggttttgt
     2581 tgtcccagtt gatctgattg atgctgtcgg gcgattcgta ggtgttgttc accccttttt
     2641 tgtcgcggta aatttcttct ccggcatata taaacggtat tccctgcgaa gtaaatacca
     2701 cagtctgggc cagtttgttg aatttttgaa tttcggcttc agtggctccc gccggtttac
     2761 gtttacgtag tttatccacc agacacatat cgtcgtggca cgaaacgtaa ttgattacct
     2821 gcgttggctg agtggcataa ggtgctttgg aatgtacgag tttagttgta tcaattcccg
     2881 gatagggtgt ggaagccaca atgccgaatt tgacggcatt ttccaggcta tcccttcccg
     2941 aaacaaatcc cgggaattcg ttcatccagt ttcctttcag cgcatcgcgg atatcgtcgc
     3001 tgaatacggc tattccgttc agtttactta catttttctt cacagcccgt ttttcttctc
     3061 caagcggtga ggcagctgct gtccagcctt ctccgtacag aataattttc ggatttacct
     3121 tatcaagagc tgcgcggatg gcgttcatag tttcgatgtc gtgtatgccc atgaggtcaa
     3181 agcggaatcc gtccacgtga tactcggtag cccagtaaac cactgattcg acgataaatt
     3241 tccgcatcat ggcacgatcg gaggctgttt cgttgccgca tcccgatgca ttagaccaag
     3301 tgctgtcggc attgaaccgg tagaaataac ccggaacgag cagattcaaa tggctttcct
     3361 cgccaacata cgtgtgattg taaacaacat ccattaccac tcctattcct tctgaatgaa
     3421 gtgattgcac catttgcttg aattccttga tacggcttac cggatttgcc ggatcggttg
     3481 aataactgcc ttccggaacg ttgtaattca ggggatcgta accccagttg tatttgtttt
     3541 ccttcagttt ggtttcatcc accgaggcat aatcgtatga tggcagcaga tgcacgtgag
     3601 tgattccaag ttctttcagg tgatcgatac cggtggactg tccggctgta ttttttgtcc
     3661 ccttttcggt gaaagccagg aattttccct tgtttttaat gcctgaattc ggtgaaaccg
     3721 agaaatcgcg catgtgcact tcgtaaagta ccgcatcggt cacgtgttcc ggcaggacat
     3781 gtttatcatt ttcccagcca tccggatttg tttcgttgat atctatgatt gcagcgcgtt
     3841 taccgttgat tcccacggct ttcacccaca tgccgggagt ttcgttgagc catttttcgc
     3901 ccattttcac ctgaaaagtg taatatttgc ctttcagatc tccttttacg ttggctatcc
     3961 aggtaccgct tttagagcgt ttcatgtctt tcatttcata agcagagcca tccagtccgt
     4021 tgtcgaaaag caacagtttt acttcgtcgg ctgctggcga ccatactctg aattttgatc
     4081 cggatggaga ataagtcagt tcaaggtcat ttccttcata cacaggaaat tcttcataac
     4141 tcttgaattt tttcccacta ccggtgcagc taatcagtat tccggatatt gccagaatta
     4201 tccatgtttt tttcatgatg atgttgtgtt atattacaaa aattacaatt atttgattcc
     4261 gtgacgctgc agtaaggctt tgatatctgg ttcctgaccc ctgaaacgtt tgtaaagcac
     4321 cataggatgt tcacttccgc ctttttcaag gatatttacc cggaaagaat cggctgtttt
     4381 tttatcaaaa ataccgtttt taaggaatac agagaatgca tctgcatcga gcacttcggc
     4441 ccatttgtag ctgtaatagc cggctgcata tcctccggag aaaatatgac tgaaagtggg
     4501 gctcattccg gtattttcca ctaccggaat tacctgtgca ggcgccattg cttccttctc
     4561 gaacgcaatg acatctcctt caaacggttt atcaatcgtg tgccatttca tatcgagcag
     4621 tccgaaactc agttggcgaa gggtaaatgt acctacgtta taattgtcgg catccttgat
     4681 tttctgaatt aattcggccg gcattttttc accggtctgg taatgtacgg caaactgatc
     4741 gagaaattcc ttttcaacgc cccagttttc cattatttgc gacgggagtt ccacaaaatc
     4801 gcggtaaacg ctcgtacccg agagtgactg gtaggtggtt ttagagagca tgccatgaag
     4861 tgcatgacca aactcgtgga gcatggtggt aacttcatcg aatgtaagca aagcgggttt
     4921 agtagatgtg ggacgggtaa aattcatgac gttgatgatg tgcggacggc tgtctttttt
     4981 tccgtccatc cactgatcct tggtgctgct catccatgct ccggcgcgtt tgccgtcacg
     5041 cggatgataa tcggtataga gtaccgccac gaattttccc ttactatcgg tcacatcgta
     5101 ggctttcact tcggaatgat acaccggaat gttcggattt tccttgaaat taagtccgta
     5161 aagcttgttt gcgagccaga atatgccttt tttcacacgt tccagttcga aataaggttt
     5221 gagcatctcg tcgttcagat taaatttctc ggctttaagc ttttcggaat aatacgacca
     5281 gtcccagggt tgaattttga aataagcacc gtttcggtcg gcataagcct gcacatcggc
     5341 atattctttt tcggcagtgg gtttatatgc atcgagcagt ttgttaagca gttcgtacac
     5401 gttgtcggta ttttcggcca tccggcgctg gagcacatgg tcggcatagg ttttatgtcc
     5461 gagcagattg gctatctgaa gacgcagatt agctattttt cgtagatttt cccggttgtc
     5521 gtaatcacct ccaatcatac attttgttga actggccagg tagagttcgc gcctcaggtc
     5581 gcggttgtct gcatatttca ttaccggtgc gtaactcgta gttttcaggt tgatcaacca
     5641 gccttctttt ccggcttttc gggcattttc ggccagcatt tccatcacat cttcgggcag
     5701 tcctttgagt gtttcgggat ttttgatgag catttcatat ttgttcgttt ccttcagcac
     5761 gttttggccg taggtaagtg tgagcatgct cagctctttg gtcagttcgc ggtatctggc
     5821 cttatcggca tccgacaggt tggcaccgtt attggcaaac ccatcatacg tatctttcag
     5881 gagggttttt tgctctgcat tcagtttgag cttgtctttc tgatcgtaaa cggatttgat
     5941 gcgttcgaaa agtttctcat tcagcaggat ttcattggag tgttcgctca tcaggggtga
     6001 aagttcctga gccacagcct gcagttcatc actggtttcg gctccgagca ggttgtagaa
     6061 aggtgaggaa acccgggcaa gcagtctgcc tgctttgtcg agcgctacga tggtattttc
     6121 gaaagttgca ggtttggggt tgttgacaag ggcttcaatt tcagctttat tttgcttcat
     6181 tgcctccaga aaagcaggtt tgaaatgttc tgtttttatt tcgtcaaacg gaacggtttg
     6241 atacggagtt ttatacggtt gtaacaatgg attggcggca aatacgcctg cagtaatggt
     6301 taatgcggtc atacacacaa ttattttctt ttttatcata ctcttttttt ttctgaataa
     6361 acttaattat acttttttta tggttcaaaa tgaaaaaaaa cctgtcaaag ttaagtacaa
     6421 ttattctaat aaacaagatt tgaattctcc ttttataaat ttaaggaaat gttgaattta
     6481 tgcttttaga caaacctttt atttagaaga atttgatatt attaaaagat tacactattt
     6541 ttgcatatat cgaaccaact aatatttcaa aaactatgaa aaaactggaa atcccttcga
     6601 taaaagcact gaatgatgtc ggggcagatt cagccggtga cttactggaa gaatcaggct
     6661 ataagtctgt gatagaaata cttaactggc cggatcagta tgcatacaag cccattacct
     6721 attttttcat cgggaggtct gccgatgctg tttttttgaa attttctgta aatggcacca
     6781 tgctgaaggc cgtttatacc gaagatcatt cacccgtcca cgaagacagt tgtgtggagt
     6841 ttttctgcat gccagaaggg gccgacaaat acaccaattt tgaattcaac tgcattggaa
     6901 cctgttcagc atcgagccga aaagggcgta atgaagatgt gattcctttt tcaaaagaag
     6961 aaatggctac catagagcgg tatgcatcca taggcaaaaa gcctttcaag gaaatggaag
     7021 gcatgtttga atgggaactg accgtaaaaa ttccgttgaa actgatgaat ctggatccag
     7081 aaaatcttcc tgaaaagatt cggggcaatc tgtacaaatg tgccgatgat accgattcgg
     7141 tacactttgt gagctgggcg cctatcaaaa cggaaaatcc cgatttccat cgtccggagt
     7201 tttttggcga aatgtatttc aaataaacct ttaccgaact gaaaaatttt ccaggcaaaa
     7261 aaactaccgg cttcgttcat ctttatattt taagaattat tattaactaa ttatttttaa
     7321 ttaaagtttt taataattat ctttgtaccc tgtgacaata atctacatca catgaaaaaa
     7381 acattttcta ctgcattatt tatcactgct tttgctgctg caggttcggc acagaatcaa
     7441 aaaccaaacg taatactgat actggccgac gatctcggtt attcggacat cgaaccctac
     7501 ggacaggaaa aaatcaaaac acccaatctg aacaggatgg ccgaaaatgg aatgcaattc
     7561 atgcaattct actgtggcac ttctgtcagc gcaccatccc gcgccagcct gatgacaggc
     7621 cttcacacag ggcatacaca cgtacgcggc aacaa
//
LOCUS       DAORKP010000112         7478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1658_length_7478_cov_9.219318, whole genome shotgun sequence.
ACCESSION   DAORKP010000112 DAORKP010000000
VERSION     DAORKP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_7478_cov_9.219318"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4777
                     /locus_tag="PLN75_06990"
     CDS             <1..4777
                     /locus_tag="PLN75_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRG03898.1"
                     /translation="QDITGLISIPEAANFTFQTDKNVVKIYPETMPENKLTLQINQGI
                     QNITGLKLDKNYTYNLSVEGTKPEIAFEKSGNILPNSEQLVLPFKAVNLWAVDVKVIK
                     IYQNNILYFLQTNTLSENSQNEIRRFGRLIKKIRIRLDEDKTMDLSRWNNFSIDLAPM
                     IKKDPGAIYLVQLSMKQDYSLYRCNGNAPVQPLITPMKSFDDDTADAEEMNKWDEHYA
                     DYYEDTDWENYNWDERDDPCKPSYYMNSNRRAETMVMASNMGIIAKSGDNGKITVAVT
                     DILTTSPLSGATVKVFNYQLQQTATAKTNADGFAEIEYGKTKPFVVTASKGKDIGYLE
                     VKDELSLSLSNFDVSGKEIRKGLKGYLYGERGVWRPGDTIHLTFILEDKNQKLPNGHP
                     VSLEIFTPTRQFYSRQVKTEGINGFYTFNIPISPTGQTGTWQAYVKVGGTNFYKGLRV
                     ETIKPNRLKVRFETDTIIDAGKGVVSGILTSQWLHGAPAANLKADVELTLSKTDKPFR
                     NFTNYSFNNPLVNFESGKHMIFQGVLSQSGTAIVNSKIPIAETAPGILQGSILSRVFE
                     TGGDMSFYSQTVYYSPYSSYVGIKSPATRENEFLETDKNLVFDVVTLNAKGIKLNRKV
                     DYKIYKLEWSWWWNTSEENLGSYVNNTAVRPLTMGSVVTTGGAAKINFQVDYPDWGRY
                     LILAKDTESGHTTGTVFYVDWPASRGRSSKTDPNGLTMLSFSTDKKEYSVGEKATVTI
                     PKSSEGRVLISIENGSHIIQREWVKTSPDSDSKYSFRVTDEMNPNFYVFATLLQPHSQ
                     QNNDLPIRMYGVVNVSVDNKNSKLTPIIAMPGELRPEKEFTVSVSEQSKKPMTYTLAI
                     VDEGLLDLTSFKTPNAWNEFYAREAIGVRTWDLFDRVLGARTGMLGPLLSIGGDEALK
                     ASSDKVNRFKPVVKFIGPFTLKSGETKSHKIKLPAYVGSVRVMVVAGGNGAYGSADKS
                     VQVTSALMTLTTLPRVLGPNEEVFLPVNVFVTDKKVKNVTVSIKTNGMLKPADGVSKT
                     VSFDKPGDKVLFFRLKADKKTGEEHISIKSSGGGESFSENIDIGIRNPNPPHIISKSA
                     LVDSKGKVLLSLHSDNTGPNDWAKLEISRLPGINFNKNLQYLLNYPHACTEQITSQGF
                     PLLFISDFTRQTDEEKKRNSEKVDEIIRILSSRQLPDGGFMYWSGDHYASEWVSTYAG
                     HFLTEARQKGFEVSEVVLSKWVQFQQKLARNWTPTNPYRNYYSLSMPQLQQAYRLYSL
                     VRAENTESGAMNRLRELKDLSIQARWQLAAAYALTGKKDVANELIFNQSTQVENYTFN
                     NDTYGTPARDQAIMMETYLLLGQTEKAMALASDVAKALSGDYISTQTAAFGLVAMQKL
                     AEKMGKGNINVSWTMNDNKMNQINTPQVFHQVNLKLSENLTVEIANQGQAKVFVRMTA
                     LTQPLDASNFPSDEETGESPLRLTASYKDVNGNPMDIKSLKQGQEFTAEVSVQNNNIQ
                     DFTDLALVQIFPSGWEIFNERMMHVNSSAGEASFSYRDIRDDRILTYFNLGAGQSKSF
                     SVRLQAAYRGTFFHPSISCQAMYAPGEQARTPAAWVKVE"
     gene            4786..7149
                     /gene="pbpC"
                     /locus_tag="PLN75_06995"
     CDS             4786..7149
                     /gene="pbpC"
                     /locus_tag="PLN75_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRG03899.1"
                     /translation="MNFIKACFKKFQSFTTWKKITFLVIFFLLIWYALCLPKPLFEKS
                     YCTVVTDRNDELLGARIAKDGQWRFPPAHSVPQKYKICLLEYEDRYFNRHFGVNPLAI
                     VRAAIQNMQNKRIVSGGSTITMQTVRLMRQKKRTVVEKIIEMILATRLEFSYKKNEIL
                     ALYASHAPMGGNVVGIDAASWRYFGHSATSLSWAEAATLSVLPNSPSLMHVGKNREEL
                     TLKRNKLLKKLSDEKLIDVTDYNLAISEPLPARPQVLPQIAPHLVTQFYLTNPGLQSK
                     SSVDKQLQLQADEVLKRWNTEFSQNGINNLAALIVDIEKNEVLTYAGNIDFEKNTAGN
                     QVDVIQSPRSTGSILKPFLYCAMLQEGLMLPRELLPDIPVNINGFAPKNFSLQYDGAT
                     PANEALARSLNVPWVVSLRKYGVAKFYDFLKKTGISTLTKTADYYGLSLILGGAEGTL
                     WEIANGFSHLARTVNDYNQSNEYFEIEDFSVQLMDNNIKRKKTSSPVFNAGAAWITLE
                     ALTNVNRPEEIDWQFIPSIRKVAWKTGTSFGFRDGWAIGVTPKYLVAVWTGNASGEGR
                     PGLTGARTSAQVMFDLFNLLPATSWFQTPYGELREASICSESGLLRGLNCPESSVDTI
                     LIPKKGQQAKTCEFHKIIHVSEDEHFQVFEQCAGNRGIKTVSQFVLPPSWEWYYKQNH
                     PNYRSLPSFSPECIGANSGQVMQFIYPYPNSVLKITKQLDGSRGKVVFELAHRNSSTK
                     VFWHLDDGFLGETSDIHKLEYAPEKGEHVLTVVDENGNAISTKFKIE"
BASE COUNT         2344 a         1625 c         1623 g         1886 t
ORIGIN      
        1 acaggacatc acagggctta tttcaatacc cgaagctgct aatttcacat ttcagacaga
       61 taaaaatgtg gtgaaaattt accctgaaac aatgccggag aacaagctca cacttcaaat
      121 caaccagggc attcaaaaca tcaccggatt aaaactggac aaaaattaca cctacaacct
      181 cagcgtggaa ggaacaaaac ctgaaattgc ttttgaaaaa agcggaaata ttctaccaaa
      241 ctccgagcag ttggttttac ctttcaaagc tgtaaatctg tgggctgtag atgtaaaagt
      301 aattaaaatt tatcaaaaca acattcttta cttcctgcaa accaacacat tgagtgaaaa
      361 ttcacaaaac gaaatccgtc gtttcggccg tttgataaag aaaatccgga tacgactgga
      421 tgaagataaa accatggacc tgagcaggtg gaataatttt tccatcgatc tggcgcccat
      481 gattaaaaaa gatcctggag ccatctacct tgttcaactt tcgatgaagc aggattattc
      541 tctttaccgg tgtaacggaa atgctcctgt acaacctcta atcacaccca tgaaaagttt
      601 tgatgatgat acggctgacg ctgaagagat gaacaaatgg gacgagcact acgccgatta
      661 ttacgaagac acggattggg aaaattacaa ctgggatgaa agagatgacc cgtgcaaacc
      721 ttcgtattat atgaacagca accgacgtgc cgaaacgatg gtgatggcct caaacatggg
      781 tattattgcc aaaagcggtg ataatggtaa aattacagta gctgtcaccg acatcctgac
      841 aaccagtcca ctttcgggtg caactgttaa agttttcaat tatcaactgc agcaaaccgc
      901 aacggccaaa actaatgccg acggatttgc agaaattgag tatggaaaaa caaaaccatt
      961 tgttgtcaca gcatccaaag gcaaagatat cggttatctg gaagtgaagg atgagctatc
     1021 gctatcgttg agtaattttg atgtgagtgg caaggaaata cggaaagggc tgaaaggata
     1081 tctgtacggc gaacgtggag tatggcgtcc gggcgacacc atacatctga cttttattct
     1141 cgaagataaa aatcaaaaac tcccgaatgg gcatccggtt tcactggaaa tttttacccc
     1201 gacacgccag ttctacagcc gccaggtgaa aaccgaaggt atcaacggat tttacacttt
     1261 caatatcccc ataagcccta cagggcaaac cggcacctgg caggcatacg tgaaagtggg
     1321 tggaacgaat ttctacaaag gactcagggt agaaaccatc aagccaaacc ggctcaaagt
     1381 acgattcgaa accgacacaa taattgatgc cggtaaagga gtggtttctg gcatactcac
     1441 ttcgcaatgg ctacacggag ctcctgcagc caatctgaaa gccgatgtgg aactcactct
     1501 gtcaaaaacc gataaaccgt tcagaaattt cacaaattat tcctttaaca atccgctggt
     1561 aaacttcgaa tccggaaaac acatgatttt tcagggtgtg ctcagtcagt cgggaaccgc
     1621 tattgtcaat tctaaaatac cgattgccga aacagctccg gggatattgc aaggcagtat
     1681 tttgtcacgt gtatttgaaa ccggcggcga tatgagtttt tattcccaaa cagtatatta
     1741 ctcaccctac agttcttacg tgggcatcaa atcacctgca acccgggaaa acgaatttct
     1801 tgaaacggat aaaaatctcg tttttgatgt ggttacactc aatgcaaagg gaataaaact
     1861 caaccggaaa gtggattata aaatctacaa actggaatgg agctggtggt ggaacacttc
     1921 cgaagagaat ctgggttcat acgtgaataa tactgctgtt cgacccctta ctatgggaag
     1981 cgttgtcaca acaggaggag cagccaaaat aaattttcag gttgattacc cggattgggg
     2041 acgttatctt attttagcta aagacacaga aagcggacac actaccggta ccgtttttta
     2101 tgttgactgg cctgcatcca ggggcaggtc ctccaaaacc gatcccaacg gactaacgat
     2161 gctttcattc tcaaccgaca aaaaagaata ttctgtgggc gaaaaagcaa cggttaccat
     2221 accgaaaagt tccgaagggc gtgttttaat aagtattgaa aatggctcac acatcattca
     2281 gcgggaatgg gtaaaaacat ccccggattc agactcgaaa tattctttca gggttaccga
     2341 tgaaatgaac ccaaatttct acgtttttgc cacgctcctg caaccacatt cacaacaaaa
     2401 caacgacctt ccaatccgca tgtatggagt tgtaaatgtg agtgttgaca ataaaaacag
     2461 taaactcaca ccgataatcg cgatgccggg tgaactgcgt cccgaaaagg aatttacagt
     2521 ttccgtttcc gaacagtcga aaaaaccgat gacctacaca ctggcaattg tagatgaagg
     2581 tctgctggat ttaacgtcat tcaaaacacc gaatgcctgg aatgaatttt atgcgcgtga
     2641 agctattgga gtacgtacat gggatctgtt tgatcgcgtt ttgggtgccc ggaccgggat
     2701 gctcggaccg ttgttgagta ttggcggcga cgaggcgctg aaggcatcga gcgataaggt
     2761 gaaccggttc aaaccagttg tgaaatttat cggtccattc acacttaaat cgggagaaac
     2821 gaaatcacac aaaataaaac taccggctta cgtcggttcg gttcgcgtaa tggtggtagc
     2881 tggtggaaat ggtgcttatg gaagtgccga taaatcagtt caggtaacga gtgcgcttat
     2941 gactctgacc actttgcccc gtgttctcgg accgaatgaa gaagtcttcc tgcctgtgaa
     3001 tgtatttgtt actgacaaga aagtgaaaaa tgtaaccgtt tccattaaaa ccaacggaat
     3061 gctgaaaccg gcagatggcg tttcaaaaac ggtttcgttt gataaacccg gagataaagt
     3121 actctttttc agactgaaag cagataaaaa aacaggcgaa gaacatatct cgataaaatc
     3181 ttcgggaggc ggagaatcat tctcagaaaa tatagacatt ggcattcgaa atccgaatcc
     3241 acctcacatt atatcaaaat cagctcttgt tgattcgaag ggaaaggtct tattatcatt
     3301 acattcagat aacaccggtc cgaatgactg ggcaaagctg gaaatatcac gtctgcccgg
     3361 catcaatttt aataaaaatc tgcaatacct gctgaattat ccacacgcat gtaccgaaca
     3421 aatcacttca cagggctttc cgctgctttt tatttctgat tttacccggc aaacggacga
     3481 agaaaagaaa cgcaattcgg aaaaagtaga cgaaataata cgaattttgt cttcgcgaca
     3541 actgcccgac ggcggattta tgtactggag tggtgatcat tatgcttcgg aatgggtaag
     3601 cacttatgcc ggtcattttc tgactgaagc cagacaaaaa gggtttgaag tttcggaagt
     3661 cgtactttca aaatgggtac aattccagca aaaacttgcc cgaaactgga ctccaaccaa
     3721 tccatacagg aattattaca gcctgtcaat gcctcagttg cagcaggcgt accggcttta
     3781 ctcgctggta cgtgcggaaa atacggaatc cggagccatg aaccgcttgc gggagttgaa
     3841 ggatttgagc atacaggccc gctggcaact ggctgcggct tatgcactca ccggcaagaa
     3901 agatgtagcc aacgaactga ttttcaacca aagtacacag gtggaaaact atacattcaa
     3961 caacgacacg tacggaacac cggctcgtga tcaggcaatc atgatggaaa catatctgct
     4021 gctcggacaa accgaaaaag caatggcatt ggcttcagac gtggctaaag ctctttccgg
     4081 tgattatatt tcgacacaga cagctgcttt cggactggtg gcaatgcaaa aactggccga
     4141 aaaaatgggc aaaggcaata ttaatgtcag ctggaccatg aacgacaaca aaatgaatca
     4201 aatcaacaca ccgcaggttt ttcatcaggt aaatttgaaa ctttcggaaa atcttacagt
     4261 agaaattgcc aaccagggcc aggctaaagt attcgtccgg atgactgccc ttacgcagcc
     4321 actggatgca tctaactttc catcagacga agaaacgggt gaatcgccac tcagattaac
     4381 agcatcttac aaagatgtga acgggaatcc gatggatata aaatcgctga aacagggtca
     4441 ggagttcact gccgaagttt ctgttcaaaa caacaacata caggacttta ccgatctggc
     4501 attggtacag atattcccct cgggatggga aattttcaac gaaagaatga tgcacgttaa
     4561 ttcttctgca ggtgaagcaa gcttcagcta tcgcgacatt cgcgacgaca gaattctcac
     4621 ctatttcaat ctgggtgcag gacagtcaaa gtcgttcagc gttcgtttgc aggccgccta
     4681 tcggggtaca ttctttcacc cgtcgatttc ctgtcaggcc atgtatgctc ccggcgaaca
     4741 agcccgcaca cctgctgcgt gggtcaaagt ggaataatct gaaaaatgaa ttttatcaaa
     4801 gcctgtttta agaaatttca atccttcaca acatggaaaa aaatcacctt cttagtgatt
     4861 tttttcctgt tgatttggta tgccctatgt ttaccgaaac ctctttttga aaaatcgtac
     4921 tgcacggtag taaccgatcg gaacgatgag ctgttgggtg caagaattgc caaagatggt
     4981 caatggcgat tcccacccgc ccacagcgtt cctcaaaaat acaaaatctg cctgcttgaa
     5041 tatgaggatc gatattttaa cagacatttc ggagtcaatc cgctggctat tgtacgcgcc
     5101 gccattcaaa acatgcaaaa taagcgaatt gttagcggcg gtagcaccat cacgatgcaa
     5161 actgtccggt tgatgcgaca aaaaaaacga actgtcgtag aaaaaatcat tgaaatgatt
     5221 ctcgcaacac ggcttgaatt ttcatataaa aaaaatgaaa tacttgccct gtatgcatcg
     5281 cacgcaccca tgggaggcaa tgttgtggga attgacgctg cttcctggag gtatttcgga
     5341 cacagtgcaa cctcactatc gtgggctgaa gccgccacac tgtcagtact gcccaactca
     5401 ccttcgttaa tgcacgttgg gaaaaaccgg gaagaactga cgctaaaacg caacaaactt
     5461 cttaaaaagc tttcggatga aaaactgatc gatgtgaccg actacaatct tgctatatct
     5521 gagccacttc cggctcgtcc acaagtattg cctcaaatag ctcctcacct ggtcacgcaa
     5581 ttttacctca cgaatcccgg cctacaatca aaatcatcag tcgataagca attacaactt
     5641 caggcagacg aagtactgaa aagatggaat actgagtttt cacaaaacgg aattaataat
     5701 ctggcggctt taattgttga tattgagaaa aatgaagtgc tgacatacgc aggtaatatt
     5761 gattttgaga aaaacactgc cggaaatcag gttgatgtca ttcagtctcc acgaagcaca
     5821 ggtagtattc tgaagccatt tctttattgt gctatgctcc aggaaggatt aatgttaccc
     5881 cgtgaattac ttcccgatat tccggtaaac atcaacggct ttgctcccaa aaatttcagt
     5941 ttgcagtacg acggagctac accggccaac gaagccctgg cacgttcgct caatgtaccc
     6001 tgggtagtgt cattgagaaa atacggcgta gcaaaattct atgattttct gaaaaaaact
     6061 ggaatttcca cccttactaa aacagctgat tattacggtc tttcactaat tctgggtggt
     6121 gcagaaggca ctttgtggga aattgcaaac ggattttcac accttgcacg cacggtgaat
     6181 gattacaatc aatcaaatga atattttgaa atagaagatt tcagcgttca attaatggat
     6241 aataatatta aaagaaaaaa aacctcatct ccagttttca atgcaggtgc tgcctggatt
     6301 actcttgaag ccctgacgaa tgtgaacagg cccgaagaaa ttgactggca gttcattcca
     6361 tcgatacgta aagtggcatg gaaaacaggt acaagctttg gtttcaggga tggttgggca
     6421 atcggagtta ctccgaaata cctggtagct gtatggaccg gcaatgcaag cggcgaaggc
     6481 cgtccgggac ttaccggcgc ccgaacttca gcacaggtga tgtttgattt atttaattta
     6541 ttacccgcta cgtcgtggtt tcagactcct tacggagaat tgcgcgaagc ttccatttgc
     6601 agcgaaagcg gacttctgag aggattgaat tgcccggaat cctctgtcga tacaatactt
     6661 attcctaaaa aagggcaaca agctaagacc tgcgaatttc acaaaattat acatgtttcg
     6721 gaagatgaac attttcaggt ctttgagcaa tgtgccggaa accgtggaat taaaacagtt
     6781 tcacaatttg tgcttccacc ctcctgggaa tggtattaca agcagaatca tccaaattac
     6841 cgctctcttc cgtcgttttc tcccgaatgt attggagcaa attcgggtca ggtgatgcaa
     6901 tttatttatc cttatccaaa ctccgtactc aaaatcacaa agcagctgga tggttcaaga
     6961 ggaaaagtgg tttttgagtt ggcacacaga aattcgtcca ctaaagtatt ctggcatttg
     7021 gatgacgggt ttttgggtga aacttccgat attcacaaac tggaatatgc tcccgagaaa
     7081 ggtgaacacg tgttgaccgt ggtggatgaa aacggaaatg caatctcaac aaaattcaaa
     7141 attgaataaa ttcacctgtt attcttagtc tttctgtttc tctttgaaac tttcgagtta
     7201 tctaaactta tttgaacccc ctaaaatgcc aattgacaga ttttttagaa aaaaaaagag
     7261 aatttgattt gttagtataa aaaaaagcag tatctttgca atcgcttttg aaattttgga
     7321 ttcagtagct cagcaggtaa aacatcccga tttcatcggg agtggtcacg gcttcaagct
     7381 ccggttgaat cactaaaaaa gcaaaataaa aaggagattc agcagctcag caggtagagc
     7441 atcccgattc catcgggagt ggtcctggct tcaagcaa
//
LOCUS       DAORKP010000113         7392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1694_length_7392_cov_9.627504, whole genome shotgun sequence.
ACCESSION   DAORKP010000113 DAORKP010000000
VERSION     DAORKP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1694_length_7392_cov_9.627504"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLN75_07000"
     CDS             <1..852
                     /locus_tag="PLN75_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRG03900.1"
                     /translation="MAVITSLEADHLDIYGTYEAVVDSFQHFASLITPGGALILNEKI
                     NLQPKLSKGVKFFTYGMNKTSDFHAENVRIIKENIYFDFVTPTETIHDIRLAVPVMIN
                     VENGVAAMALAWLSGVTNEELRTAMSTFSGIYRRFNIVYYNPEVVYMDDYAHHPSELK
                     ASISSIRQLYADRKITGIFQPHLYSRTRDFAPEFAEALSELDELILLDIYPARELPIE
                     GVTSELIMKDVSIPDKTLTSKENLLILLDQKELDVLVTFGAGDIDKMVPEIKKYLKER
                     FGNNSEK"
     gene            853..1590
                     /locus_tag="PLN75_07005"
     CDS             853..1590
                     /locus_tag="PLN75_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HRG03901.1"
                     /translation="MNKLLKYILIISAMALCVSYAVLANVKGTASDDRKVCSSLNVTI
                     ADYKEKKLISNEEIALLLKTKELNPIGQSLGKIRTKTIEDVIEKHPMVRRAECYKTPK
                     GEVEIVIEQRNPVLRVAGTENYYVDDLRKTMPVSLNFAAYVPVATGRITRKTATGPLF
                     DFARYISRDPFWNDQIAQININEKMQVELIPRVGEHIIILGTFDRYKQKLEKLRKLYL
                     YGLNEAGWNAYSTINLQFRDQVVCTRK"
     gene            1760..3043
                     /gene="ftsA"
                     /locus_tag="PLN75_07010"
     CDS             1760..3043
                     /gene="ftsA"
                     /locus_tag="PLN75_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRG03902.1"
                     /translation="MNKNPLVYIDFGGSRIKAIAGFVQDNHALKVMGEQEKVSDDVKS
                     GIVEKISGAAFNVSKITRLLQNSLKLDQITRVSTSVNARTMKHFTTSVEGRIMNLVTE
                     KLLKDLEKECIDEVESDKISAFECIPLAYYIDGQYIDEPPGKKGSMLRIDYNVIIGNK
                     LVKESLERSIERTGIAVDYIHLGIEAAATAILEDKDREEGCAVISFGATTTTLGVYCE
                     GKLQEMSVIPLGGKNITKDIQELGISFEHAELLKTKTGCAMEEKVEKPVYVQIPNENP
                     GNEPVKISTSFLARIIEARLEEMLEPIFDQINMIPYPLRWGIIITGGAAKLKYLPEFI
                     EQKTGCTVRVGSHAEWLSEDTDEKFYDPAYSQAVGALLLSDELINKQAEIQQAAIKSK
                     LPNSFIKKVKNKITVGMESLFKYDELEKEQNEQQK"
     gene            3078..4409
                     /gene="ftsZ"
                     /locus_tag="PLN75_07015"
     CDS             3078..4409
                     /gene="ftsZ"
                     /locus_tag="PLN75_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRG03903.1"
                     /translation="MDFLDDNLPLNLPLANSSIIKVIGVGGGGGNAVNHMYNQGIADV
                     SFVVCNTDNQDLVRSPVPIKVQLGKNITQGLGAGGRPEVARQAAEESVEDIRRMLADN
                     TKMVFITAGMGGGTGTGASPVVAKVAHDMGILTVGIVTIPFAFEGNQKIRQALEGVVA
                     LSEYVDAILVINNEKLKQIYPDLELSNAFAKADDVLTNAAKAIAEIITIPGYINTDFA
                     DVSTIMRDGKVAIMNTGFASGENRITKAIEDALNSPLLNTNDVNGAGKVLLSLYCSKS
                     NQIKMEEISQIHEFMAKVGENVTVIWGASFDESLGDEVKITLIATGFDVKDIPGMPAS
                     VLKKYKMDEKKDNNESGDFENNIFDEELEEHKKTAEAEISKAIVELYGGMIKSETPKE
                     TKPEEIFQEVIQFDAVQEEELPVVNLFDIDDIDDLKKVEQIPAWKRRFLKK"
     gene            4483..4938
                     /locus_tag="PLN75_07020"
     CDS             4483..4938
                     /locus_tag="PLN75_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRG03904.1"
                     /translation="MEKLFEQISNDIKTAMLAREKVALEALRGIKKEFLEAKTAKGSD
                     GILHDEQAVKIMQKMAKQRRETAQIYTEQNRPELAEKELAEAAVIEQYLPKQMTDTEL
                     EAALIEIIAQTGAAGPQDMGKVMGAATRQLAGKADGRMISEKVKSLLSR"
     gene            complement(5037..5624)
                     /locus_tag="PLN75_07025"
     CDS             complement(5037..5624)
                     /locus_tag="PLN75_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRG03905.1"
                     /translation="MSLSTSKTRKKLIDVSRELFALRGKKNITMNDIAEASKKGRRTL
                     YTYFKNKDEVYKAVIENELNLVIIALEENAKKAIDAFEKLRNHIIIHLDLIKQSVTRN
                     GTLRADFFKDIYEVERSRRKMDVIELKIIRNILDEGLATGKFKTMDTEIASSIIMYSL
                     KGLEIPYIRQNLTSEFEKNKHEIVEFVFNGISMES"
     gene            complement(5706..6659)
                     /locus_tag="PLN75_07030"
     CDS             complement(5706..6659)
                     /locus_tag="PLN75_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRG03906.1"
                     /translation="MMETTLRRTRTAVISALPKAGRTTVWLLKIIIPISLAVSILQYT
                     GVIEVIARYLSPVFSVIGLPGEAAIVFITSIMLSLYAPIAIIATLPLDPRDVIILAVM
                     CLIAHNMIVETAVQSKTGSRYITMFLLRIISAFVAAFFLNLLLPQTVETSQIAADAEV
                     YQSFADMLLAWLISSFWLIVKILLIVTSLMLLQNILKEFKILDVISRTLAPLMSLMGL
                     PRNCSFLWFVAHILGLTYGSAVMIAEVESGELSREDANLLNHHIAVNHSTLEDTLLFA
                     AIGVPLLWMIVPRFVLAIVIVWFVRLVHRLKLKPELKTNKS"
     gene            7182..>7392
                     /locus_tag="PLN75_07035"
     CDS             7182..>7392
                     /locus_tag="PLN75_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRG03907.1"
                     /translation="MRTSFITEQQEIDKIIESCSHCFVGMTEKDGSPYVIPMNFGYAD
                     GIIILHSGPHGKHLNLLDADNRVCVT"
BASE COUNT         2450 a         1573 c         1556 g         1813 t
ORIGIN      
        1 atggctgtca tcacgtcgct cgaagcagat catctggata tatacggcac ttatgaagcc
       61 gtagtggata gttttcagca ttttgcatcg ctcattaccc ccggaggagc actcattctg
      121 aatgaaaaaa tcaacttaca gccaaaactc agtaaaggag tcaagttttt cacttacgga
      181 atgaacaaaa cctccgattt tcatgcagaa aacgtaagaa taatcaaaga aaacatctat
      241 tttgattttg taacacccac agaaaccatt cacgacatcc gcctggcagt gcccgtgatg
      301 ataaacgtag aaaacggagt ggcagccatg gcgcttgcct ggttaagcgg agttactaac
      361 gaagaactca gaaccgccat gtctacattc tcgggtattt accgcagatt caacatcgtt
      421 tactacaacc cggaggtggt ttacatggac gactatgcac atcatccgag cgaactgaag
      481 gcaagcatat catccatccg tcagctctac gccgaccgga aaataaccgg gatattccag
      541 ccgcacctgt acagccgcac gcgtgatttt gctccggaat ttgctgaagc attgtcagaa
      601 ctggatgaac tgattctgct cgatatttat ccggcgcgcg aacttcccat agagggcgta
      661 acctcggaat taataatgaa agatgtaagc atccctgata aaacgctcac ttctaaagaa
      721 aatttactaa ttttgttaga tcaaaaagaa cttgatgtgc tcgtaacttt cggtgccggc
      781 gatattgaca aaatggtgcc cgaaatcaaa aaatacctga aagaacgatt tggaaacaat
      841 tcggaaaaat aaatgaacaa actgctcaaa tacatactga ttatctccgc aatggctctg
      901 tgtgttagtt atgcggtgct ggccaatgta aaaggtacag catcggatga cagaaaggta
      961 tgctccagtc tgaatgttac cattgcagat tacaaagaaa aaaaactgat cagcaacgaa
     1021 gaaattgcct tgctgctcaa aacaaaagag ctgaatccaa tcggtcaatc actgggaaaa
     1081 atccgaacca aaaccattga ggatgtaatt gaaaaacatc cgatggtgcg aagggcggaa
     1141 tgttataaaa caccaaaggg agaagttgag attgtgatcg aacagcgcaa cccggttttg
     1201 cgggtcgcag gcaccgaaaa ctattacgtg gatgatttga gaaaaaccat gccggtttca
     1261 cttaattttg cagcctacgt gccggttgca actggcagaa ttacccgcaa aacagctaca
     1321 ggacctcttt ttgactttgc ccgttacata agtcgcgatc cattctggaa cgatcagata
     1381 gctcagatta acatcaatga aaaaatgcag gtagagttga taccacgcgt cggcgaacac
     1441 ataatcattc tcggaacttt tgaccggtac aaacaaaaac tcgaaaaact ccggaaactt
     1501 tatctttacg gactcaacga agcaggctgg aacgcctact ctaccatcaa tctgcaattc
     1561 agagatcagg tagtatgtac cagaaaatag ctcagtgaac agaaaatgaa aaattttaaa
     1621 ctctaactta ttttgatttt ttatctgaca acattgattt tctacacaca ttaaatatat
     1681 ttttctgaat ttcagacaga tatatttgag tgtttttcaa aaaaaacgta aattttataa
     1741 cgtctaattt tccataagta tgaacaaaaa tcctcttgta tatattgact ttgggggatc
     1801 acgaataaaa gcaatagccg gatttgtgca ggataatcat gccttgaagg tgatgggtga
     1861 acaggaaaaa gtgagcgacg atgtaaaaag cggtattgta gaaaaaatca gcggcgctgc
     1921 tttcaatgtg agtaagataa ccagattgct tcagaactca ttgaaacttg atcagattac
     1981 cagggtaagc accagtgtga atgccagaac catgaaacat ttcacaacca gtgttgaggg
     2041 tagaattatg aatctggtta ccgaaaaatt actgaaagat ttagaaaagg aatgtatcga
     2101 tgaagtagaa tctgacaaaa tcagtgcatt tgaatgcatt ccgcttgctt attacataga
     2161 cggacaatat attgacgaac cacccggaaa aaaaggctcc atgctgcgta ttgattacaa
     2221 cgtcatcatc ggcaataaac tggtaaaaga atcgctcgaa cgaagcatcg aacgcaccgg
     2281 aattgccgta gattacattc atctgggcat tgaagccgca gccactgcca tacttgaaga
     2341 caaagaccgc gaagaaggat gcgctgtaat ctcttttggt gccaccacca ccacactggg
     2401 agtctattgc gaaggaaaac ttcaggaaat gagcgtaata ccgctcggag gcaagaacat
     2461 caccaaagac attcaggaac tcggtatcag ttttgaacat gcagagttat tgaaaacaaa
     2521 aacgggctgt gcaatggaag aaaaggttga aaagcccgta tatgtgcaaa ttccaaatga
     2581 aaatccgggc aatgaaccgg taaaaatttc cacttcgttt ctggcaagaa ttattgaagc
     2641 acgtctggaa gagatgctgg aacccatttt cgatcagata aatatgattc catatccact
     2701 ccggtggggc atcatcatta ccggtggcgc agcaaaactt aaatatctgc cggagtttat
     2761 cgagcagaaa acaggctgta ccgtccgggt gggaagtcat gccgagtggc tttcggagga
     2821 taccgacgaa aaattctacg atccggccta ctcgcaggct gtaggagctt tgttactatc
     2881 tgatgaactg atcaataaac aggctgaaat tcagcaggct gccataaaat ccaaattacc
     2941 gaacagtttt attaagaaag ttaaaaacaa aataactgtc gggatggaat cgctcttcaa
     3001 atacgacgaa ctggaaaaag aacaaaacga acaacagaaa taaaaaaaag gatttaatca
     3061 tacttaaact aaataatatg gattttttag atgataatct gcctttgaat ttgccgctgg
     3121 ctaattcatc catcataaaa gtaatcggag ttggaggtgg aggcggaaat gccgtgaacc
     3181 acatgtacaa ccaggggatt gccgatgtat catttgtggt atgcaacacc gataatcagg
     3241 accttgtacg ctcacccgta cctattaaag tgcaactggg caaaaacatc acccaggggc
     3301 tgggtgcagg cggacgcccc gaagtagcac gccaggcagc cgaagaatcg gtggaagaca
     3361 tcagacggat gcttgcagat aacaccaaaa tggtattcat taccgccggt atgggcggag
     3421 gcaccggcac aggagcttca cccgtagtag ccaaagtggc tcacgatatg ggaatactta
     3481 ccgtaggcat cgttaccatc ccctttgcct ttgaaggaaa tcagaaaatc cgtcaggcac
     3541 tcgaaggcgt ggtggcactc agcgaatacg tggatgccat tctggtaatc aacaacgaaa
     3601 aactcaaaca gatttatccg gatctggagc tctccaatgc ctttgccaaa gccgacgacg
     3661 tactcaccaa tgccgccaag gcaatagctg agatcattac gattccgggc tacatcaata
     3721 ccgactttgc cgacgtgagc accataatgc gcgacggaaa ggtagctatc atgaacaccg
     3781 gatttgcttc cggcgaaaat cgcatcacca aagccattga agatgccctc aactctcccc
     3841 tgctgaacac caacgatgtg aacggcgccg gaaaagtact gctgagtctt tactgctcca
     3901 aaagcaacca gattaaaatg gaagaaattt ctcaaataca tgaattcatg gccaaagttg
     3961 gcgaaaatgt cacagtcatc tggggagcaa gtttcgacga gtcactcggc gacgaggtta
     4021 aaatcaccct tatagcaacc ggatttgacg tgaaagacat acccggaatg cctgcatccg
     4081 tactgaaaaa atacaaaatg gatgaaaaaa aggacaacaa tgaatccgga gattttgaaa
     4141 ataatatttt tgacgaagaa ctggaggaac ataaaaaaac cgccgaagcc gaaataagca
     4201 aagccattgt agagctttac ggtggcatga tcaaatcaga aacaccaaaa gaaacgaaac
     4261 cggaagaaat attccaggaa gtaattcaat ttgatgccgt tcaggaagag gaactgccgg
     4321 tagtgaacct tttcgacatc gatgacatcg atgacctgaa gaaagtggaa caaattccgg
     4381 cctggaaaag acgatttctg aagaaataaa gaattttcat cggaagcaaa acaaattcac
     4441 ccgatctatt tcaactattc aaatattcaa cttataaaaa caatggaaaa gttattcgaa
     4501 caaataagca acgatataaa aacagccatg ctggcccgcg agaaagtggc actggaagca
     4561 ttgcgcggca taaaaaaaga atttctggaa gccaaaactg ccaaaggaag cgacggcata
     4621 ctccacgatg agcaagccgt aaaaatcatg cagaaaatgg caaaacaacg cagggaaaca
     4681 gcccagattt acactgaaca aaaccggccc gaattggcag aaaaagagct agcagaagca
     4741 gccgttattg agcaatacct gcccaaacag atgaccgaca ccgaactgga agccgctctg
     4801 atcgaaatca tcgcacagac aggagctgca ggacctcagg atatgggaaa agtgatggga
     4861 gcagctacca gacagcttgc cggtaaagcc gacggaagaa tgatttcaga aaaggtcaaa
     4921 tcgctactga gcagatagta tcacccggaa agtatccgcc tgatgcatac ataaaatcag
     4981 cgggagcacg attgattcat gctcccgctg attttttata taaagatagt tcagaatcag
     5041 gattccatac tgattccgtt gaacacaaat tctacaattt cgtgtttgtt tttctcaaat
     5101 tcggaggtca gattctgtcg aatatacgga atctcaagcc ctttgagtga gtacatgata
     5161 atactagatg ctatttcagt atccatagtc ttaaattttc cggtagcaag cccttcatcc
     5221 agaatatttc tgatgatttt cagttcgatg acgtccattt tacgtcgcga gcgttccacc
     5281 tcgtaaatat ccttaaaaaa atcagcgcgc agcgttccgt tgcgtgtgac cgattgtttt
     5341 atcaaatcca ggtgaatgat gatgtgattt cttagcttct cgaatgcatc aatcgctttt
     5401 ttggcattct cttcgagcgc aatgatgacc agattcaatt cattctctat caccgcttta
     5461 tacacttcgt ccttgttctt aaagtaggtg taaagcgttc ttctgccctt tttcgaagcc
     5521 tctgcaatgt cgttcatggt aatgtttttc ttaccccgca atgcaaacag ttcacgcgac
     5581 acatcaatca gttttttcct ggttttagaa gtggataatg acatgatatg tatgtaataa
     5641 tttgagtata aagttattca tttgacccga aatctccaaa aagccccgat ttgatttcag
     5701 caaatttaac ttttgttcgt tttcaattcc ggctttaatt tcagccggtg caccaggcga
     5761 acaaaccaca caataacgat ggccagcaca aaacggggca caatcatcca gagcagcggc
     5821 accccgatgg cagcaaaaag tagcgtatct tcaagcgtag agtgattcac ggcaatgtga
     5881 tggttgagca gattggcatc ttccctggaa agttcgccac tctccacttc tgctatcatc
     5941 acagccgatc cgtaagtaag acccagaata tgagccacaa accagagaaa agaacagttg
     6001 cgcggtaagc ccatcaggct catcagcggc gcgagcgtcc gcgaaatcac atcaagaatt
     6061 ttgaattctt tcaggatatt ctgcaggagc atcagactcg tcacaatgag cagaatcttc
     6121 actatcagcc agaacgaact gataagccag gccaacagca tgtcagcaaa tgactgatac
     6181 acttcggcat ctgctgctat ctgactcgtt tcgaccgttt gtggtagcaa tagattcagg
     6241 aaaaaagccg ccacgaaagc actgatgatt cggagcaaaa acatggtgat ataacgcgag
     6301 ccggttttgc tttgtacggc cgtctccact atcatgttgt gagctatcaa acacattacg
     6361 gcaagaataa tgacatctct gggatcaagc ggaagggtgg caatgatggc aatgggagca
     6421 taaagcgaca gcataatact ggtaatgaat acgatagccg cttcgccggg caaaccaatg
     6481 actgaaaata caggagaaag atagcgggca atcacctcaa taacaccggt gtattgcaga
     6541 atactcacag ccagactgat ggggatgatg attttcagca gccaaacggt tgttcttcct
     6601 gctttgggta aagctgaaat cacggctgta cgtgttctac ggagtgttgt ttccatcatt
     6661 tgatttattt gaaatgtatt taaaaaaaga aaggttgtct cacgacaacc caatcttcaa
     6721 ttaaccttaa atctaatacc atgaaaaaca cgatgcaaag ataacgcgtt tttatgttat
     6781 aaaacatagt tttgatgaaa aaatcgtcgt ttttaactct aattaactta attgcgtatt
     6841 ttaaccggtt ttctgaagtt ttataagcat tgtaatacac tgaaaatatc gatcagaaaa
     6901 aaatgtcaat cagaaacttt ttttaaaaaa agaaaggttg tctcacgaca acccaatctt
     6961 caattaacct taaatctaat accatgaaaa acacgatgca aagataacgc gtttttatgt
     7021 tataaagcat agttttgatg aaaaaatcgt cgtttttaac tctaattaac ttaaaaatac
     7081 catcaatctc tctttttgaa gatctgttca cagtatttta aaaagtttca acagttatat
     7141 atcggacaaa attatctatt tttgtgcaaa aatcagtcac aatgagaaca agttttatta
     7201 ccgaacagca ggaaattgac aaaataattg aaagttgcag ccattgtttc gtaggaatga
     7261 ccgaaaaaga cggctctcca tacgtcatac caatgaattt cgggtatgcc gacggcatta
     7321 tcatcctcca ctcgggtcct cacggaaaac acctgaatct gctcgatgcg gacaacaggg
     7381 tttgtgtgac at
//
LOCUS       DAORKP010000114         7212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1802_length_7212_cov_10.043175, whole genome shotgun sequence.
ACCESSION   DAORKP010000114 DAORKP010000000
VERSION     DAORKP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_7212_cov_10.043175"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1240
                     /locus_tag="PLN75_07040"
     CDS             368..1240
                     /locus_tag="PLN75_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRG03908.1"
                     /translation="MGKKDNFWNNNYLGYILKNILIATGLLMGLILATLLLINIYTNH
                     GSSEKVPNLKGTTLEEARIMLKRHNLEAEIIDSVYLKDKKQGTIIEQNPAPNSIVKPG
                     RPIYLIINSKSARKITLPDVRDVSLRQAEAMIKSMGVNIATVQYAPSEYRDLVLDIKY
                     KGQTLLPGSKIPEGSSVVLIAGDGYGGGTSSGVPSVKGLDLKSATEIINASNFLMGGI
                     IYDVTPNGNEDQYIVYQQRPEPGDPVSTGTAIDLFLSKDRSRLNEALPPMVKRKSTED
                     KQEKEKVEDIEEFF"
     gene            1266..2345
                     /locus_tag="PLN75_07045"
     CDS             1266..2345
                     /locus_tag="PLN75_07045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRG03909.1"
                     /translation="MEEDYLEDIDSEMEMDDTASGGEMYEHYRFEVDKGQSLLRIDKY
                     LTDKIAGISRNRIQDAADAGNILVNRKPSKSNYRVKPLDIITIVLAYPRIALEITPQD
                     IPIRIVYEDDELLVIDKQPGLVVHPGVGNYEGTLLNAIAFHLKDNPYFDPNDSRVGLV
                     HRIDKDTSGLLLIAKTPNAKTNLGKQFFDKTTRRTYNALVWGNVKEDEGTIIGDLGRD
                     PRDRMLFTVFPEGENPLAKHAVTHYKVLERLGYVTLVECRLETGRTHQIRVHMKHIGH
                     TLFNDARYGGNEILKGNRTAKYQQFVKNCFEICPRQALHARTLGFTHPTTGRDMNFDS
                     ELPDDMTQLIEKWRRFAGIISTPDL"
     gene            2673..3533
                     /locus_tag="PLN75_07050"
     CDS             2673..3533
                     /locus_tag="PLN75_07050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRG03910.1"
                     /translation="MKQSSGNSLELYNELSYELSREITRKYSTSFHSATTLFGAETRR
                     AIQSIYGFVRLADEIVDTFHDTDKQYLLEKFEADYYDAYKQGISLNPLLHSFQSTVKK
                     YGINDEFIQDFLLSMKADIEKKEYKSKSEMNDYIYGSADVVGLMCLKVFCNGNAQLFQ
                     SLLIPAMRLGSAFQKVNFLRDLREDLETLGRSYFPEVSLNKPDEKTKAILVEDIEKDF
                     ATALEGIRKLPDDSRLAVMVAYRYYTRLLKNIKKTPAQELMKRRIRVSDSKKVMLLVT
                     SVIKHKLNLE"
     gene            3533..4075
                     /gene="idi"
                     /locus_tag="PLN75_07055"
     CDS             3533..4075
                     /gene="idi"
                     /locus_tag="PLN75_07055"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRG03911.1"
                     /translation="MERENMVQLVDEQDNPTGLMEKMAAHRDPHLHRAISVFILNSSG
                     EWLLHQRSSHKYHSRELWSNACCTHPYEGESYPEAAARRLQHEMGMNCPLTPMFSFIY
                     KAQIDDELTEYEYDQVFAAVTDDLPQLNEEEVKDWKYIRFDDLKLDVENHPEKYSAWF
                     KIIYLRVHSELNGLIFPKNT"
     gene            4078..4563
                     /locus_tag="PLN75_07060"
     CDS             4078..4563
                     /locus_tag="PLN75_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03912.1"
                     /translation="MVLNILMLLIGFSMMEFVAWSNHKYVMHGFLWRWHKDHHVRDRQ
                     KVTQSELAKTEANRFEKNDLFFIVYATPAIVLMVIGIALQLMPALFLSIGITLYGMIY
                     FLIHDVAIHQRLNIPFLIHSRNKYLQAIIRAHVAHHRPKKKTDFYNYGLLIFPKKFWN
                     E"
     gene            4567..5256
                     /locus_tag="PLN75_07065"
     CDS             4567..5256
                     /locus_tag="PLN75_07065"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRG03913.1"
                     /translation="MGFYLTILLLSFSIPFLLSFDRKLHFFTQWKQAIPAILIVAVAF
                     ILMDMLYTHLGVWGFNPDYYLGFQIFNLPLEEVLFFILIPYACVFLHEVLKLYFPKIH
                     LRKKHSNRVSVILLVISIVLLVFAFPKLYSVYSLGLVVVAALLAIFFDRENEMGYFYF
                     SFLVMLIPFVIVNGFLTGSFAGNEVVWYDNSENSSLRLLTIPVEDFGYGFGMLYFVVW
                     IKKRLEIGKIW"
     gene            5240..5887
                     /locus_tag="PLN75_07070"
     CDS             5240..5887
                     /locus_tag="PLN75_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRG03914.1"
                     /translation="MEKSGKYSIRDWVVIGALVIFYTVGFIGFELPLSHSLFVKLTPL
                     ALLLSVLVLIKYDKGEACRKMLLYLAFVFAGGFFIEVVGVNTGLIFGHYNYGEGLSIK
                     LFDTPLMIGLNWALMVYLSAAVVLSFRRNFLYQVLLSSAIMVAYDVIMEQVAPKMDMW
                     NWAGETIPLQNYLLWATIAVVFHAVRYFIRLEVKNRMALPILIIQSIFFLLIWLI"
     gene            5889..6515
                     /locus_tag="PLN75_07075"
     CDS             5889..6515
                     /locus_tag="PLN75_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRG03915.1"
                     /translation="MLKSRHHFIVYPFFQHYTRYLLRTRYHTVSIENHFADNGNAVLL
                     IGNHVGWWDGFWAMYLNLKVLKRKFHFMMLEEQLLRYRFFNYTGAFSVNKGSRSVVES
                     IRYASELLNDSKNMLLMYPQGRLHSMYEHHFQFEKGIERMLKNRERKVQVVFSANLVD
                     CFTEPKPSLSIYTENYEGAFTVEALEHAYNDFYARCLEKQFAKAENKD"
     gene            6537..>7212
                     /locus_tag="PLN75_07080"
     CDS             6537..>7212
                     /locus_tag="PLN75_07080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRG03916.1"
                     /translation="MIYILYILVGFAAVQFVVALSNLLFQEKLSEKGLPQTGVERNDV
                     SVLIPVRNEEKNIGSLLSDLLQAGDNFREIIVFDDESTDGTAEVVSKIASADSRVHLL
                     TSAGLPEGWLGKNHACHRLALEAQGEFFLFLDADVRISKGLIDKMVNYVKKKKLDLLS
                     IFPTQEMTGWQEKITVPNMHYILLSLLPLPLVRLAPFSSLAAANGQCMLFRSETYREM
                     MLHEKFR"
BASE COUNT         2016 a         1627 c         1686 g         1883 t
ORIGIN      
        1 tcacttgcgg gagtttggac gggtacctgc ctttcaatgc tggtcatgtt acaactgatg
       61 gaatcataat gcaccgcaca aacacttaca ttccagtagt atcgctctga gatgaactta
      121 acagcttatc ttcggtgtta aatggactcg gtaataactt cttgctgtgt atcagcatac
      181 aacataaagc atgacaatcc tctgtttcgc gttaggtgag tccaaacgat taaggagtaa
      241 cgtctggcat ggtcttctta tttttttgat tttccaaaga ctatccgttg aaatagtaca
      301 attatttacg tatttttgta aattaattac tcgtttctaa aaaaatcaaa cgatatatac
      361 ctggattatg gggaaaaagg ataacttctg gaacaacaat tacttaggct acatacttaa
      421 aaacatactg atagcaaccg gtttactgat gggtttgata cttgctaccc ttctgctcat
      481 taacatttac accaaccacg gaagctccga aaaagttccg aacctgaaag gcactacgct
      541 ggaagaagcc aggattatgc ttaaaaggca taacctggag gcagaaatta tcgattcggt
      601 ttatctgaaa gacaagaaac agggcaccat catcgagcaa aatccggcac ccaacagcat
      661 agtgaaaccg ggcaggccaa tctatctgat aatcaactct aaatcggcac gcaaaatcac
      721 cctgccggat gtcagagacg tttcgctgcg gcaggccgaa gccatgatca aatccatggg
      781 agtgaacatt gccactgtgc agtatgcccc gtcggaatac cgcgacctgg tgctggatat
      841 caaatacaaa ggacaaacat tgcttcccgg ttccaaaatt ccggaaggca gttcggtagt
      901 gctcatagcc ggcgacggat acggtggagg cacttcatcg ggagttcctt cggtgaaagg
      961 gctcgatctg aagagcgcca ctgaaattat caacgcctcc aacttcctaa tgggaggcat
     1021 catatacgat gtgaccccca acggcaacga ggatcaatac atcgtctatc agcagcgccc
     1081 cgaacccggc gatccggttt caaccggcac tgccattgat ttgtttctga gcaaggaccg
     1141 ctcacgcctc aacgaagctc tgcctcccat ggtgaaacgg aaaagtaccg aagataagca
     1201 ggaaaaggaa aaggtggaag acattgagga attcttttaa tttgtttgca aggcaataaa
     1261 atacagtgga agaagactat ctggaagaca tagattccga aatggagatg gatgacacgg
     1321 cttcgggcgg tgaaatgtat gagcattacc ggtttgaagt ggacaaaggt cagtcgctgc
     1381 ttcgtatcga taaatacctg acggacaaga tagccggcat ctcccgcaac cgcatacagg
     1441 atgctgccga tgccggcaac atactggtaa acaggaaacc gtcaaaatca aactacaggg
     1501 ttaagccgct ggatatcatc accatcgtgc tggcataccc acgcatagcg cttgaaatta
     1561 ctccgcagga tatacccatc cggattgtat atgaagatga cgaactgctc gtcatcgaca
     1621 agcaaccggg gttggtggtg catcccggag tgggcaatta cgaaggtacg ctgctcaatg
     1681 ccatcgcctt ccacctgaaa gataatccgt atttcgatcc gaacgactcg cgggtgggac
     1741 ttgtgcaccg tatcgataag gatacttcgg gtttgctgct gatagccaaa actccgaatg
     1801 ccaaaacaaa cctgggaaaa cagtttttcg ataaaaccac acggcgaacc tacaacgcac
     1861 tggtgtgggg aaacgtgaaa gaagacgaag gtaccatcat cggcgatctg gggcgcgacc
     1921 cgcgcgaccg gatgctcttc accgtatttc ccgaagggga aaatccactg gcaaaacatg
     1981 ccgtgaccca ctacaaggta ctcgaacggc tgggatacgt tacgcttgtg gagtgccggc
     2041 tcgaaacggg gcgaacgcat cagattcgcg tacacatgaa acacatcggg cacaccctct
     2101 tcaacgatgc ccgatacggc ggcaacgaga tactgaaagg caaccgaacc gccaaatacc
     2161 agcagtttgt gaaaaattgc ttcgaaatct gtccgcgtca ggcactgcat gcccgtacgc
     2221 tgggcttcac tcaccccact acgggccgcg atatgaactt tgacagcgaa ctgcccgacg
     2281 atatgactca gctgatagaa aaatggagaa gatttgcagg aattatttca acaccagact
     2341 tgtaaatgct tttataaaaa atttgattat ccgaaaagtg tactgataaa attagttttt
     2401 gcctgtaagc catatttaat tggctacgcc aaacgttgtt tcagcccaat agcaacgccc
     2461 gggaaatcga ttgatatatg caatttgtga cctgaaaggg caaattaaat ttttaagttt
     2521 ggctttcagc cgattattca atttagaaca cattgcggat aattagaaaa aaacttctct
     2581 cattaaattt gtaattagaa caaaaatata ttatttttgt taataataga acacatttag
     2641 tctaactaaa agtcatattt atcagaaaat ccatgaaaca gtcatcaggc aattctttgg
     2701 agctgtacaa cgagttatcg tatgagctaa gccgggaaat taccagaaag tacagcacct
     2761 cgtttcactc ggctaccaca cttttcgggg cagaaacccg ccgtgccatc cagagtatct
     2821 acggctttgt gcgtttggct gatgaaattg tggatacttt tcacgatacc gacaagcagt
     2881 atttgcttga aaaatttgaa gcggattact acgacgccta caagcaggga atcagtctga
     2941 atccgctgct ccactccttt cagtccaccg taaagaaata tggtatcaac gatgaattca
     3001 ttcaggattt cctcttgagc atgaaagccg acattgagaa aaaggaatac aaaagcaaat
     3061 cggaaatgaa cgattacatc tacgggtcgg ccgatgtggt gggtctgatg tgcctgaaag
     3121 tattctgcaa cggcaatgca cagttgtttc agtcgctgct cattccggcc atgcggctgg
     3181 gttctgcatt tcagaaagtg aatttcctgc gtgacctcag agaagatctc gaaaccctcg
     3241 ggcggtcgta ttttccggaa gtttcactga acaaacccga cgaaaagacc aaagccatac
     3301 tggtggagga catcgaaaaa gactttgcga ccgccctgga gggtatccgc aaattgcccg
     3361 atgactcgcg cctggctgtg atggtagcct acaggtacta cacccggcta ctgaaaaata
     3421 tcaaaaaaac acccgctcag gaactcatga aacgtcgtat ccgcgtatcc gacagcaaga
     3481 aagtgatgct cctcgttacc tcggtcatca aacacaaatt aaacttggaa taatggaaag
     3541 agaaaacatg gtgcaactcg ttgacgagca agacaatccc acgggactga tggaaaaaat
     3601 ggccgcacat cgcgaccctc atctgcacag ggctatttct gttttcattc tgaatagcag
     3661 cggcgaatgg ctcctgcatc agcgttcgtc tcacaagtat cactcgcgtg aactttggag
     3721 caatgcctgc tgcacccatc cgtatgaggg cgaaagctat ccggaagctg ccgccagacg
     3781 tctgcagcac gaaatgggaa tgaactgccc gcttactccg atgttcagtt tcatctacaa
     3841 agcccagata gacgacgaac tgactgaata tgaatacgat caggtgtttg cagccgtcac
     3901 agacgacctc cctcagctca acgaagagga ggtaaaagac tggaagtaca tccgtttcga
     3961 cgatttgaag ctggatgtgg aaaatcaccc ggaaaaatac tctgcatggt tcaaaatcat
     4021 atatctgagg gttcacagtg aattaaacgg actaattttt cctaaaaata catagccatg
     4081 gtacttaata ttcttatgct gctcatcggt ttctcaatga tggaatttgt ggcctggtcc
     4141 aatcataagt atgtgatgca cggttttctg tggcgctggc acaaagatca ccatgtgcgc
     4201 gaccggcaaa aggttaccca atccgagctg gcaaaaaccg aagccaaccg cttcgagaaa
     4261 aacgacctgt ttttcattgt ttacgccact ccggctattg tacttatggt catcggcatt
     4321 gcactgcagt tgatgcctgc gctgtttctg agcatcggaa tcaccctgta cggaatgatt
     4381 tattttctga ttcacgatgt ggctattcat cagcgcctga acatcccttt tcttatacac
     4441 tccagaaata aatacctgca agccattatc cgggctcacg tagcacatca ccgaccaaaa
     4501 aagaaaaccg atttttacaa ctacggattg ctcatctttc cgaaaaaatt ctggaacgaa
     4561 taagctatgg gattctatct gacaatattg ctgctctcgt tttccattcc ttttctgctc
     4621 agttttgacc ggaaactgca cttcttcacg cagtggaaac aggccatacc tgccattctg
     4681 atagttgcag tggcttttat cctgatggat atgctctaca cacatctggg agtttggggt
     4741 tttaatccgg attattattt gggcttccag attttcaatt taccactgga ggaggtatta
     4801 ttctttatcc tgatacccta tgcctgcgtt tttttgcacg aagtgctgaa gctttatttc
     4861 ccgaaaattc atttgagaaa aaaacacagc aatcgggtat ccgtcattct gctggttatc
     4921 agcattgtcc tgctggtgtt cgcttttcct aaactctatt cggtttacag cctcgggctg
     4981 gtggtggtgg ctgcgttgct ggccattttt ttcgacaggg aaaacgaaat gggctacttt
     5041 tatttcagtt ttttggtaat gcttatcccg tttgtgatag tcaacggctt cctgactgga
     5101 tcatttgccg gcaacgaagt ggtgtggtat gataattccg aaaactcctc gctgcggctg
     5161 ctcaccattc cggtggaaga tttcggatac ggattcggga tgttgtattt tgtagtatgg
     5221 attaaaaaac ggttggaaat tggaaaaatc tggtaaatat tcgattcggg attgggtagt
     5281 aataggtgct ctggtcattt tctacacggt aggatttatc ggtttcgagc tgccgctgtc
     5341 tcattcgctt tttgtgaaac tcaccccgct ggcgctgctg ctttccgttt tggtgctgat
     5401 taaatacgac aagggtgagg catgcagaaa aatgttgctg tatcttgcgt ttgtttttgc
     5461 cggaggattt tttatcgagg tagttggtgt aaacaccggg cttatttttg gtcattacaa
     5521 ctatggtgaa ggtctgagta tcaaactgtt tgatacaccg ctgatgatcg ggctcaactg
     5581 ggctttgatg gtttatcttt cagcggcagt ggtcttgtcg tttcgccgca attttctata
     5641 ccaggtgctg ctctcttccg ccattatggt ggcctacgat gtaatcatgg agcaggtagc
     5701 tcccaaaatg gacatgtgga actgggcggg tgagaccatt cccctgcaga attacctgct
     5761 ttgggcaacc attgcagtcg tatttcatgc tgtcaggtat ttcattcggc tggaagtgaa
     5821 aaacaggatg gcgttgccta ttctgataat ccaaagtata ttttttctgt taatctggct
     5881 gatataacat gctgaaatcc aggcatcact tcatagttta tccgtttttt cagcactata
     5941 cgcggtattt gctcagaacg cggtatcata cggtttccat cgaaaatcat ttcgccgaca
     6001 atggaaacgc ggtgctgctg ataggcaatc atgtgggctg gtgggacggg ttttgggcca
     6061 tgtatctgaa tctgaaagta ctgaagcgca agtttcactt tatgatgctg gaagagcagc
     6121 tgctaaggta tcgttttttc aactatacgg gtgcattttc ggtgaataag ggaagccggt
     6181 cggtggtgga aagcatccgc tacgcctccg aactgctcaa cgacagtaaa aacatgcttc
     6241 tgatgtatcc acagggaaga cttcactcga tgtacgaaca tcattttcag tttgaaaaag
     6301 ggatagaaag gatgctgaaa aacagagaac gaaaagttca ggtggttttt tcggccaacc
     6361 tggtggattg ttttaccgaa ccgaaacctt cgctgtccat ttacacagaa aattatgaag
     6421 gagcattcac agtagaggct ctggagcacg catacaacga tttttatgct cgctgtcttg
     6481 aaaagcaatt tgcaaaagcg gaaaataaag attaaacaaa atattattct caaacattga
     6541 tatacatact ttacatcctg gttggttttg ctgccgtaca atttgtagtg gcactgtcga
     6601 atctgctttt tcaggaaaaa ctttccgaaa agggattgcc gcagaccggt gtggagagaa
     6661 atgacgtatc ggtcctgatt ccggtccgaa acgaggaaaa gaacatcggc agtctgctga
     6721 gtgatttgtt gcaagcaggc gacaattttc gggaaatcat cgtgttcgac gatgaatcga
     6781 ccgacggaac tgccgaagtt gtaagcaaaa tagcttccgc cgacagcagg gttcacctgc
     6841 taacgtccgc cggcctgccg gaaggatggc tgggcaaaaa ccacgcctgc caccggctgg
     6901 cgctggaggc tcagggagaa ttctttcttt ttttggatgc cgatgtgcgg ataagtaaag
     6961 ggctgataga caaaatggta aactacgtga agaaaaagaa actggatttg ctaagcatct
     7021 ttccgactca ggaaatgacg ggctggcagg aaaaaattac cgtgccgaat atgcactaca
     7081 tattgctcag cttgcttccg cttccgctgg tgagacttgc cccgttttcg tcgctggcag
     7141 ccgccaacgg acagtgcatg ctttttcgct ccgaaacgta cagagaaatg atgcttcacg
     7201 aaaaattccg ga
//
LOCUS       DAORKP010000115         6968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1938_length_6968_cov_8.894691, whole genome shotgun sequence.
ACCESSION   DAORKP010000115 DAORKP010000000
VERSION     DAORKP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_6968_cov_8.894691"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLN75_07085"
     CDS             complement(<1..558)
                     /locus_tag="PLN75_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03917.1"
                     /translation="MKKSIISFVLLVIFISIANRAEAQNVYVLQNGSRVEFFSALTNV
                     MSSMQDGDTLYLPPKPIDASLNITKRVAIIGAGYHPDTSKVTGITKITGEVVFAKKSK
                     GSSMTGVRVEGRVLVQDSSITVTRCNVQFVEAQNGAVPISQVYFGDCIIRDVVRNFNN
                     VVTNALIERCIIGGQIEGGNKTNNLM"
     gene            720..1892
                     /locus_tag="PLN75_07090"
     CDS             720..1892
                     /locus_tag="PLN75_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRG03918.1"
                     /translation="MITAITSTLPMFVCLFWVMLLLIDRQQMNLSKRFLVFFFSVSFV
                     NYFSHTAYFNHQYQLYTVFDSIWCFTSLSVFPLYYLYIRLLTLDARVKWKWALVLIPS
                     LSVALLSAVIYLLMSPDEVQTFVHGVLYSEPGYEPPYSALIRLQLFRMSLFKVVFVIQ
                     VLIVIFFGLRHIMKYNARIKDFYSDTEKKDLTPIKWLLVVFIFASLVSLISSGIGKTY
                     FISHDWLLFLPSVTHSLFLFGVGYAGYKQNFTIEHYQQDLNQYELHPAGVAPLAHEKI
                     KNEDYPAQLIYLLEKDEIYKNADLRITDVAKMLHTNRTYTSRLMHDVFQSNFADFINK
                     YRVKKSEELIRNDIENEYSLEDIATLSGFSSESSFYRSFKKEKGTTPGDFRKGLKK"
     gene            1990..2823
                     /locus_tag="PLN75_07095"
     CDS             1990..2823
                     /locus_tag="PLN75_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRG03919.1"
                     /translation="MKIKLLFFALIVFSVSLNAGKTTKIVILHTNDTHSQVEPLGSND
                     MGGYARRMGVINQIRSEEKNVLLLDAGDFLQGTPYFNFYGGRVEGTALKMMGYDAVTL
                     GNHEFDNGIDSLVNVLKTAKIDVVNTNFDFSNTPLQPLVQPYKIIKRGGVKIGVFGLG
                     VNLNGLAFQKNFAGLTLKEPIASALQTSDYLKNTLKCDLVICLSHLGVSSLDSTPTDY
                     DLAAASTNIDVIIGGHSHKMLVNETAQNALGKPVVIAQMGKSGQNLGRIDVTMEKKPK
                     K"
     gene            complement(2872..3831)
                     /locus_tag="PLN75_07100"
     CDS             complement(2872..3831)
                     /locus_tag="PLN75_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRG03920.1"
                     /translation="MHRTKNRIFIFVKWLVLLAAYSFLVYKLAHVEYWNELKISFLNP
                     EIQRFVYFAAILFLMPLNWAIETAKWQLLLSGTVSQSFGDALKSVLAGLNTGFVTPNR
                     IGEFAGRILLLPPAHRPAGVAMSVINSVTQNIIIVVVGLAGAVVYFSEHYSNLTLKNY
                     LIILFSVISITLLVYFLFPSFIGKLKTDRWSENLRNAVNSLAAIKRNKLSAVLGISAV
                     RYCVFCLQFYLMLLFFNIEISPLQALAAIPVMYLLITFTPSLAAAEPAIRGSAAVFIF
                     SVYSPDDVGILLTGILIWLVNFVVPMLVGSVLIGIAPGSDKNE"
     gene            complement(3812..4321)
                     /locus_tag="PLN75_07105"
     CDS             complement(3812..4321)
                     /locus_tag="PLN75_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRG03921.1"
                     /translation="MRKVFILTGKFISDIIDFFYPPFRRYFSLQFFRYGVSGAGNMVF
                     DWLLYSFVYNVVLQKQMLNLGLVTLSSPIAALFITFPITLFTGFLLQKYVTFTASEMR
                     GHKQLVRYMLVVALNLVINYTGLKILNGVLGLWPLASKMIITVITTLFSYFSQKKFTF
                     RIKNAPDKK"
     gene            4498..5277
                     /gene="rsmA"
                     /locus_tag="PLN75_07110"
     CDS             4498..5277
                     /gene="rsmA"
                     /locus_tag="PLN75_07110"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRG03922.1"
                     /translation="MAQVRAKKYLGQHFLRDLEIARRIAESLPLREAMPVLEIGPGTG
                     VLTRFLLENPLINLTAVEIDRESVDYLHIHYPTLKLIEGDFLKMDLNELYDDKFCVIG
                     NFPYNISSQIFFKVLDYRDKIPCVCGMLQKEVAERIASKPGKKAYGILSVLIQAFYTV
                     DYLFTVSEHVFDPPPKVKSAVIRFTRNEVQHLDCDEKLFKTVVKTAFNQRRKTMRNSL
                     KPLVGKENPMYEDAIFDKRPEQLDVESFVYLTNLVEKALKI"
     gene            5372..6319
                     /gene="corA"
                     /locus_tag="PLN75_07115"
     CDS             5372..6319
                     /gene="corA"
                     /locus_tag="PLN75_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRG03923.1"
                     /translation="MSELRLFYHEDNKIKIAKSLDILTKIDVKSIIWIDLNNVALDVE
                     TRLEQFLKIYIQEDEEMEEIEISSRYIETDDSLVANSNFLLDNFTLEPVSFILKNGLL
                     VSVRDVDLRSFNETVKKIFANSKAFPTGFHVLVALLETRVEYDADVIEDTTDLISNLS
                     KSLNAVEEPDEDVLIRIKDLQEKVMVIRQTIIDKQRIVSNMLKSRLFPRELLPRLTMI
                     IKDISSLFEYTRFGFERLDYLQDTFLGMVNIQQNKIIKIFTVVSVIFLPPTLIASMYG
                     MNFKFMPELDWKLGYPFAILLMVVFSAAVLLYFKRNKWL"
     gene            6601..>6968
                     /locus_tag="PLN75_07120"
     CDS             6601..>6968
                     /locus_tag="PLN75_07120"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG03924.1"
                     /translation="MLSFFLALSGFAQQTITGTVTSSEDGLPVIGASIVVKGTSRGVS
                     TNFDGAYTLSASRGQILVFSYVGMIPREETVGESNVINVTLEPSAEQLEEVVVTAMGV
                     QTEKKKLNFAVQSVGADALT"
BASE COUNT         2133 a         1431 c         1425 g         1979 t
ORIGIN      
        1 catcaggtta tttgttttat tgcctccttc aatctgccct ccgataatac atctttcaat
       61 taatgcattg gtaaccacat tgttgaaatt tctgaccaca tcccggataa tacaatcacc
      121 gaaatatacc tgcgaaatgg gtacagctcc attttgagct tctacaaact gtacattaca
      181 ccttgtaacc gtaatggagg agtcctgaac cagtacgcgg ccttctactc ttacgcccgt
      241 catggaggaa cctttgcttt tctttgcaaa tacaacttct cctgtaattt ttgtaatgcc
      301 cgtaaccttg gatgtatccg ggtgataacc ggcaccgatg atggctaccc gtttggtaat
      361 gttcaaactg gcatctatag gttttggagg caggtacagc gtgtcaccgt cctgcatgct
      421 gctcattaca ttggtcaatg cggagaaaaa ctccaccctg cttccgtttt ggagtacata
      481 cacattctga gcttcagccc tgttggcaat tgaaataaaa attaccaaca gtacgaacga
      541 aataatagat tttttcatgg caatcggttg ttttgtaagt tatcacttga aatatttttt
      601 tcaaaaatag caaatagttg tgttaatcaa ttgtcaaatc gtaaaaatat gttgtcaaat
      661 cgtaaaaact tttttaaaac aggaaaaata catatatttg tgaaatattt attacccata
      721 tgataactgc aatcacttct accctgccaa tgtttgtttg cctgttttgg gtaatgcttt
      781 tactgatcga caggcagcag atgaatttat cgaaacgttt tcttgtcttt ttttttagtg
      841 tttcgtttgt caattatttt tctcacacag cctatttcaa tcaccaatac cagctatata
      901 ctgtttttga cagtatttgg tgctttactt ctctatccgt atttccgttg tattacctgt
      961 atatacgttt actgactctg gatgcccgcg taaagtggaa atgggcactg gtgcttatac
     1021 cttcgctgtc ggtagcgtta ttgtctgcgg ttatctattt attaatgtca cccgatgaag
     1081 tccagacttt cgtacacggc gtgttgtatt cggagccggg ctatgagcct ccatactcag
     1141 cgttgattcg tttgcagttg ttccggatgt cgcttttcaa agtggttttt gtgatccagg
     1201 ttttaattgt gatatttttc ggtctcagac acattatgaa gtacaacgcc agaatcaaag
     1261 atttctattc ggatactgaa aagaaagatt taactcccat aaagtggctt ctggtagttt
     1321 ttattttcgc ctcacttgtt tcactcattt cttcgggtat aggtaagacg tatttcatca
     1381 gccacgactg gctgttgttc ctgccatcag tcacacattc cctgttcctt ttcggagttg
     1441 gttatgccgg ttacaagcaa aattttacca ttgagcatta tcagcaggat ttgaatcagt
     1501 acgaattaca tccggcggga gttgcacctt tggctcatga aaaaataaag aacgaagatt
     1561 accctgctca gttgatatat ttgttggaaa aggatgaaat ctacaaaaat gccgatttgc
     1621 gcataacgga tgtggccaaa atgctccata caaaccgtac gtacacctcc agactcatgc
     1681 atgatgtttt tcaatctaat tttgcagatt ttatcaataa atacagggta aaaaaatcgg
     1741 aagaactgat cagaaatgat atcgaaaacg aatactctct ggaagatatc gctaccttat
     1801 ccggattttc aagtgaaagc agtttttatc gttcgttcaa aaaagaaaaa ggaactacac
     1861 ccggtgattt cagaaaaggt ctgaaaaagt gaccttcggt ctttggagtg ttttcacaac
     1921 tccaatgaaa aaaaaagtaa actattattt cgtcccattt gtttcaatat cctataaaag
     1981 taccgatcta tgaaaatcaa actattattt tttgcactga ttgttttttc tgtttctctt
     2041 aatgccggaa aaaccacaaa aattgtcatt ttgcacacca acgatacaca cagtcaggtg
     2101 gagcctctcg gaagcaatga tatgggcgga tacgcccggc ggatgggtgt aatcaatcag
     2161 atacgctcag aagaaaaaaa tgtgttactt ttggatgccg gtgattttct gcagggaacg
     2221 ccttatttca atttttacgg cggaagggta gaaggtaccg cgttgaaaat gatgggatat
     2281 gatgctgtaa cgctcggtaa tcatgagttt gacaacggca tcgactcgct ggttaatgta
     2341 ctgaaaacag caaaaatcga tgtggtaaat accaatttcg acttcagtaa tacacccttg
     2401 cagccgctcg ttcaacccta caaaattatt aaaaggggag gcgtgaaaat aggagtattc
     2461 ggactgggag taaatctgaa cggactcgca tttcagaaaa attttgccgg actcaccctc
     2521 aaagaaccca tagcatcagc acttcaaact tcggattacc tgaaaaatac gttgaaatgt
     2581 gacctggtga tctgcctgtc gcacctgggt gtaagcagtc ttgacagtac acctaccgac
     2641 tacgatctgg cagcagcatc caccaatatc gatgtaatca tcggagggca ctcacataaa
     2701 atgctggtga acgaaactgc ccaaaatgcc cttggaaaac cggtagtgat tgctcagatg
     2761 ggtaaaagcg gacagaatct cggcaggatt gatgtgacaa tggagaaaaa accgaagaaa
     2821 taaatatttt cagtaattat tatgaatgag taacggaact ttttgaatct gttactcatt
     2881 tttgtcagat cccggtgcaa tacctattaa aacgcttccg acaagcatgg gtaccacaaa
     2941 atttaccagc cagataagta tgccggttaa caaaatcccg acatcatccg gggaatagac
     3001 gctgaaaata aacacagctg ccgacccgcg gatggccggt tcggcggctg ccagtgatgg
     3061 ggtgaacgtg ataagcagat acatgaccgg aatggccgcc aaagcctgaa gcgggctgat
     3121 ttcgatatta aaaaacagaa gcatcaggta aaactgaaga caaaatacgc agtagcgcac
     3181 tgctgaaatc cctaataccg ctgataattt attccgtttg attgctgcga gcgagtttac
     3241 tgcatttctt agattttcgg accacctgtc tgtcttcaat ttgccgataa acgacgggaa
     3301 taaaaaataa accaaaagcg ttattgaaat aaccgaaaat agaattatca ggtagttttt
     3361 cagagtaagg ttactgtagt gttctgaaaa atataccaca gctccggcca gtccaactac
     3421 cacaattatg atattctgtg tcacgctgtt gattacgctc atggccacac ccgcggggcg
     3481 gtgagccgga ggaagcagaa gaattctgcc tgcaaattcg ccgatgcggt tgggtgtgac
     3541 aaatccggtg ttcagacctg ccaggactga tttcagcgca tcaccgaacg attgcgaaac
     3601 agttccggaa agtaggagtt gccatttggc tgtttcgata gcccagttga gaggcatcag
     3661 aaataaaatt gccgcgaaat atacaaatcg ttgaatttca ggatttagga aagagatctt
     3721 cagttcattc cagtattcga catgtgccag cttatacacc aaaaaactgt aagccgccaa
     3781 taaaacaagc cattttacaa aaataaagat cctatttttt gtccggtgca ttttttatac
     3841 ggaatgtaaa tttcttttgc gagaaatagc tgaacaaggt tgtgataacc gtaataatca
     3901 tcttggaagc cagcggccac aagccaagca ctccgttgag aattttcagg ccggtgtagt
     3961 ttatcaccag attgagcgct actacaagca tataacgcac aagctgcttg tgacctctca
     4021 tttcagaggc ggtgaaagtg acgtatttct gaagcaaaaa tccggtaaaa agcgtgatcg
     4081 gaaaggtgat aaaaagagct gcaatgggcg aactcagggt aacaagtccg agattaagca
     4141 tttgtttctg aagcaccaca ttgtatacaa acgaatacaa aagccagtca aaaaccatat
     4201 tacccgcacc cgaaacgcca tatcgaaaga attgcaggct gaagtacctc cggaaaggcg
     4261 gatagaagaa atcaatgatg tcactgataa atttgcctgt gagaataaat acttttctca
     4321 tttcaataga gtgcgaaaaa aatcgttttt ttaggataat tgaataattt tacctacttt
     4381 tgccggactt gcaaaaatta cagatgcaaa gatactaaaa aaccgccaac tctgtaaaac
     4441 aatttttatt acaacaattt ctatttgaac tgaatatgcc tgacgactat caccaatatg
     4501 gcacaagtac gagcaaaaaa atacctggga caacatttcc ttcgggatct ggaaattgcc
     4561 cgacgtattg cggagagttt acctcttcgc gaggctatgc ctgtgcttga aattgggccc
     4621 ggtacaggcg ttcttacccg ctttctcctc gaaaatccgc tgattaactt aactgccgta
     4681 gaaattgacc gcgaatcggt agattatctg cacattcatt atccgacact caaactgatt
     4741 gaaggagatt tcctgaaaat ggatttaaat gagctctatg acgataaatt ctgtgttatc
     4801 ggcaattttc cctacaatat ttcgagccag atttttttca aagtgctgga ttaccgcgat
     4861 aaaataccct gtgtgtgcgg aatgctccag aaagaggtgg cagagcgaat tgcatcaaaa
     4921 cccgggaaaa aagcctacgg aatcctgagc gttctgatcc aggcatttta tacggtcgat
     4981 tatcttttca ccgtgtctga gcatgtcttt gaccctccgc cgaaggtcaa atcggctgtt
     5041 attcgcttta cccgaaatga agtgcaacat ctcgattgcg acgagaaact cttcaagaca
     5101 gtagtgaaaa cagccttcaa ccagcgacgc aagaccatgc gcaattcttt gaaaccgctt
     5161 gtaggaaaag aaaatccaat gtatgaagat gctatttttg ataaacgtcc cgaacagctg
     5221 gatgttgaaa gctttgtata cctgactaat ctggtggaaa aagcactgaa aatctaacac
     5281 ttcttcaagc tacgcattca gcgcaaaagg tcttaaaatt ctcattatta atcatctgca
     5341 aagcgaaagc ttacaagaaa ggaggtaaat catgtcggaa ttgagacttt tttatcacga
     5401 agacaataaa ataaaaattg ccaaaagtct tgacatctta accaaaatag atgtgaagag
     5461 tattatctgg attgatctga acaatgtggc actggatgtg gaaacacgcc tcgaacagtt
     5521 tctgaaaatc tacatccagg aagacgaaga aatggaagaa attgaaatca gctccaggta
     5581 catagagacc gacgatagtc tggtggcaaa ctccaatttc ctgctcgata attttaccct
     5641 tgagccggtc tcatttatcc tcaagaacgg attactcgtt tccgtgcgtg atgttgacct
     5701 gcgttcgttc aacgaaacgg taaaaaaaat ctttgcaaac tccaaagcat tccccaccgg
     5761 ttttcatgtg ctggtagcac tcctcgaaac ccgcgtggaa tatgacgccg atgtaattga
     5821 agacaccacc gacctcatat caaatctcag taagtcgttg aatgccgtag aagaacccga
     5881 cgaagatgtg ctcatacgca tcaaggacct gcaggaaaaa gtaatggtaa tccgccagac
     5941 cattatcgat aagcagcgca tcgtatccaa tatgctcaag agccggttgt tcccgcgtga
     6001 actgctccca cgcctcacca tgattatcaa agacatcagc tcgctttttg aatacacacg
     6061 gtttggattt gagcgactgg actacctgca ggacacattt ctcggtatgg tcaacattca
     6121 gcagaataaa atcatcaaaa tttttaccgt ggtatcagtc attttcctcc cacccacact
     6181 catcgcaagc atgtacggca tgaacttcaa atttatgccc gaactggact ggaaactcgg
     6241 atatccattc gctatcctgc tcatggtggt cttttcggca gctgttttac tgtatttcaa
     6301 gcggaataaa tggctttaga acccataata attagtcatt ttcattaaaa tgaattattg
     6361 aaaattcggg aagtatttta ttattaccgt tatttttgtt aaaaagcagc attttgtcat
     6421 aaaaatgctg ttttttttgt tttatgtata gagggatgta ttttcgatta aattacctta
     6481 ataataccta aaagaattaa ttaaactgta aaatttttgt attttcggag attgataaat
     6541 gaaaaaagta tcaagtgtaa agtaaataat aatattatta tgaagaaatg ttgtttctta
     6601 atgctgagtt tttttcttgc attatccggt tttgcacagc aaaccattac cggtacagta
     6661 acttcatcag aagacggatt accggtgata ggagcttcga tagtggtgaa aggaacctca
     6721 cgcggagtat ccaccaattt tgacggtgct tatacattat cagcttcaag aggacaaatc
     6781 ctggtttttt cctacgttgg aatgataccc cgggaagaaa cggtaggtga atcgaatgtt
     6841 atcaacgtga cactcgaacc atcggcagag cagctcgaag aagtggtagt gactgctatg
     6901 ggtgtacaga cagaaaaaaa gaaactgaat tttgcggtgc aaagcgtggg agccgatgcg
     6961 ctgaccga
//
LOCUS       DAORKP010000116         6950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_1949_length_6950_cov_9.661494, whole genome shotgun sequence.
ACCESSION   DAORKP010000116 DAORKP010000000
VERSION     DAORKP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_6950_cov_9.661494"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLN75_07125"
     CDS             <1..408
                     /locus_tag="PLN75_07125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRG03925.1"
                     /translation="KGVFFIMTITYNKKNYMTTNQIAELSKNGHTIGFHSWDHTQAIK
                     YGNDSILKKNIIEPRKKLEEIIGKPVEYFAYPYGLTDERTTAQMNDYFKMSFILSTKQ
                     DSINPIQSVRRMIAPEWKPQSLLRAMEKTFSRR"
     gene            complement(451..1101)
                     /locus_tag="PLN75_07130"
     CDS             complement(451..1101)
                     /locus_tag="PLN75_07130"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRG03926.1"
                     /translation="MKTIKHLPLFIFIGFILSSCSLWRLSNRNMMSIERGMTKTEIKR
                     MLGNPDYRNFDREGEEWVYTTTEKNLIVGFYNDIVESMTTFPTGTYYGRNSYPEMSNS
                     SYPAYPENNPNMGNSPAVMSDKEFQDLYTTANRQPFREDKMTSIRNGVYGRAFTCRQC
                     VRMMSLFTFDDEKLQVFNIMAPNILDWENQHLILDNFKFVSSRDKAMQTIQSIRKR"
     gene            complement(1348..3690)
                     /locus_tag="PLN75_07135"
     CDS             complement(1348..3690)
                     /locus_tag="PLN75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRG03927.1"
                     /translation="MNKKIGILLLAAVFVLIGCNQKKSGENLDSSIEKKIDKIMADMT
                     LEEKVGQMAQFTLDVIGKGPNVFSSDEPFQIDQAMLDTVIGKYKVGSILNTANNRART
                     PEVWEYIVRTIQERAIKETGIPVIYGIDSNHGTTYTAGGTFFPQEIGMAATFNTALTE
                     EGSRISAYETRASNIPWTFNPTMDLGRDARWSRQWESYGEDPYLNARMAVASVKGFQG
                     PDHNNIGRDYIAACIKHYMGYGVPVSGKDRTPAVISERELREKFFEPFRASIVEGKAL
                     SLMVNSGIINGVSTHADHRLITEWVKNELNFDGVIVSDWADIPNLFNRDHIAADYRGA
                     VKAAINAGVDMVMEPYNVAFCPTLVQLVKDKEVPMSRIDDAVRRVLRMKFRLGLFDRP
                     NWSVADYPKFGSKEHEAAAKKAADESITLLKNVNNTLPLKQGARILVTGPNANSMRTL
                     NGGWSYSWQGEKTEEFAQGYNTILEAMRNKFGEGNVRYAAGVEYKMDGQYFEELPPQI
                     GQAVAAASGVDYIVLCVGENSYCETPGNLEELVLSENQTALAKALLKTGKPVILVLNE
                     GRPRIIRTIEPESKAIIQLYLPGNYGADALADVLAGDINPSGKLPYTYPKFEQGLLTY
                     DHKPSQNIDGKMEGAYDYGAQTSVQYPFGFGLSYTTFEYSNLSVDKKEFISGDSLTVS
                     VDVKNTGSVIGKEAVMLFSSDLVASLTPDVRRLRSFEKIELNPGESKTVKLSISADDL
                     AFVNELGKWTLEEGDFRLQVGNQVDVVKCKETKIWKTPNK"
     gene            complement(3758..4894)
                     /locus_tag="PLN75_07140"
     CDS             complement(3758..4894)
                     /locus_tag="PLN75_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRG03928.1"
                     /translation="MNFFLILTLLLLSNCGSKNGTGTDEPKPNTPVVWTFSPETLADF
                     PADGESQKITITTNTAWKVTSDQNWCITSASGGFPAGSFFNVIVGKNPTDKVRTATLT
                     FTAKETTKTFSVKQAAGTGQTGGTGEHSGFVPTGYNLVWWDEFNDTKLGNGKNPLPKE
                     SIWWYETGASGWGNNEIQNYVPGFYGTDTSAVISNGTLKIIAKKRASEVISIRMNSKD
                     SWLYGYFEARMKMPVGKGTWPAFWMMPKNFNKWPDDGEIDIMEFVGYEPNVVHSTVHT
                     AAYNHSIGTQKGNKTSVSNASTEFHVYSLEWTPTYIKGFADGKEYFKFSNDNQNNKAT
                     WPFNAPFYLKLNMAWGGNWGGAQGIDPAALPATYEIDYVRVYQK"
     gene            complement(5275..6783)
                     /locus_tag="PLN75_07145"
     CDS             complement(5275..6783)
                     /locus_tag="PLN75_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03929.1"
                     /translation="MKKIIKNLSWMLMAVLFFAACNPVEDASYALGDMTPVKAEDVSF
                     TITPSAKSPNIVTLTNTTPHKGVISVVWTVDVKGDLQTFFKDKIELTLPEAGDYPVML
                     TIYNPDGTAVSKNSTLKIANSDFSLVSSEAYINLTGGMENPAGKVWVFDQYNNFAKEV
                     ADATGKNVKGHMGLGPQGSLGQEWWGAGPNEKGSWKMYDFKFNFIQAGVKLNITNGGE
                     GYGRKASSASVGGFTVTGENGDDVFFPYNGGAYTFSIDETGANPILKLSGNAFMGYYC
                     GSQEYEVFYQTDKVMALRVNNTVEGQDWVFVYTLQELNVPPPPVVKEPKAVPLSEDFE
                     GATPKVILTHDAMGDKSAVVDNPLPVPLNTSNKVYRYQKPAEFYSNMRFTAADYKFDL
                     TKQNKIKIKVFIPSYNDYTTDYATAGSWISNAKLQPQLAIKLQNSEHPAPWETQTEIV
                     KADLVKDKWIELTYDFSGVASRTDYDRIVIQFGAEGHAGPGFFFFDDFTFSE"
BASE COUNT         1840 a         1507 c         1456 g         2147 t
ORIGIN      
        1 aaaggggtgt tttttattat gaccatcact tacaacaaga agaactacat gacaaccaat
       61 cagattgcgg aactttcaaa aaacggacac accattggtt ttcacagctg ggatcataca
      121 caggcaataa aatatggcaa cgactccatt ctgaaaaaaa acatcatcga accgagaaag
      181 aaactggaag aaattatcgg aaaaccggtt gaatattttg cataccctta tggattgacc
      241 gatgaaagaa ccactgctca aatgaatgat tatttcaaaa tgtcatttat actttcaaca
      301 aagcaagatt cgataaaccc tattcagtcg gtcagaagaa tgattgcgcc tgaatggaag
      361 cctcaaagcc tgctcagggc catggaaaaa acatttagcc gaagataatc ccgaatgact
      421 tttagcgggg tttttttatt gacgtgatac ttatcttttc cggatggatt gaattgtttg
      481 cattgccttg tcgcgcgatg aaacaaattt gaaattatcc agaatgaggt gttggttttc
      541 ccaatcgagt atgtttggcg ccataatgtt gaatacctgt aatttctcat catcaaatgt
      601 aaacaggctc atcatgcgca cgcactgacg gcaggtgaac gcacgaccgt aaactccatt
      661 tcggatagat gtcattttat cttccctgaa aggctgacga tttgcagtag tgtataaatc
      721 ctgaaactcc ttatcgctca ttacagctgg agaatttccc atgtttgggt tattctccgg
      781 atatgccgga tagcttgaat tgctcatttc cggatatgaa tttctaccat aataagttcc
      841 ggtgggaaaa gtggtcatac tttccactat gtcattataa aacccgacaa tcagattttt
      901 ttcggtcgta gtgtaaaccc attcctctcc ttcacggtca aaatttcggt agtccggatt
      961 tcccagcatt cgttttattt cagttttggt catacctctt tctatgctca tcatgtttcg
     1021 gttgctcaac ctccataggc tgcagctgct aagaatgaag ccgataaaga taaataacgg
     1081 aaggtgcttt atagttttca tattcagaaa gttggagttt ttttttgaaa attgtgtttt
     1141 aggagccgta aatcatctat aacttctaaa agtcaacaaa aactatgcta aagaaaaatg
     1201 caggagaatc aaacacaggg tgggtcggaa aaatgattga atatgaatat tccgggcact
     1261 caactgttcc tgtgccgtaa aaaagaacag cagcctcttt tattagactg ctgattcctt
     1321 tttttaaaaa acatcctggt ttactatcta tttattaggt gttttccaga tttttgtttc
     1381 cttgcatttt accacatcta cctgattgcc aacttgcagg cggaaatcgc cttcttcaag
     1441 cgtccatttt ccaagttcat tcacaaatgc aaggtcatcg gcagaaatac ttaatttcac
     1501 cgttttagat tcgccggggt tcaactcgat tttttcaaaa gagcgcaaac gtctaacgtc
     1561 aggcgtgaga cttgccacca aatcactgct gaaaagcatc accgcttctt tcccaatcac
     1621 gcttccggtg tttttcacat ctacgcttac tgtcagactg tcgccggaaa taaattcttt
     1681 tttatccaca gaaagatttg agtattcaaa agtagtatag ctcaatccaa atccgaacgg
     1741 atactgaacc gaagtctgag ctccgtaatc ataagcgcct tccatttttc cgtcgatatt
     1801 ctggcttggt ttgtggtcgt aagtgagaag tccctgttca aattttggat aggtgtaagg
     1861 cagttttcca cttggattaa tatcgcctgc aaggacatcg gccagtgcat cggctccgta
     1921 gttgcccggc agataaagct ggataattgc tttggattcc ggttcaatag tacggataat
     1981 gcgcggacga ccttcgttca acaccaaaat aaccggcttg cctgttttga gcaatgcttt
     2041 tgccagggca gtctgatttt ctgaaagcac cagctcttca aggtttccag gagtttcgca
     2101 ataggaattt tcacccacgc aaagtacaat gtaatctact cctgatgcag ctgctactgc
     2161 ctgtccgatt tgcggaggaa gttcttcaaa atattgtcca tccattttgt attccactcc
     2221 ggcagcatat cttacattgc cttcgccgaa tttattcctc atggcttcca gaattgtgtt
     2281 gtatccctga gcaaattctt cggttttttc accttgccag gaatagctcc aaccaccgtt
     2341 cagcgtgcgc attgaattgg cgtttggtcc tgtgaccaga attcgagcac cttgtttcaa
     2401 aggaagagta ttattcacgt ttttcagcaa agtaattgat tcgtcagcag cttttttagc
     2461 ggccgcttca tgctctttgc ttccaaattt tggataatct gctacgctcc aatttggacg
     2521 gtcgaagagt cccaatctga atttcatgcg cagtacgcgt cttacggcat cgtcgatacg
     2581 gctcatcgga acttctttgt cttttacaag ttgtaccagg gtagggcaga aagcaacatt
     2641 gtaaggctcc atcaccatat caacgccggc attgatagct gcttttacag cgcctctgta
     2701 atcagcagca atgtggtcgc gattgaaaag gttaggtatg tcggcccagt cggaaacgat
     2761 tactccgtca aaattcaatt catttttcac ccattctgtt atcaaccggt ggtcggcgtg
     2821 ggtagaaacg ccgttgataa tacccgagtt caccatcagc gagagagctt ttccttcaac
     2881 aatagaagcc ctgaaaggct cgaagaattt ttcacgaagt tccctttcag aaataactgc
     2941 cggtgtacga tctttaccgg aaacgggcac accatatccc atgtagtgct tgatacaagc
     3001 tgcaatataa tcgcgtccaa tgttgttgtg gtcgggtccc tggaaacctt ttactgatgc
     3061 aacggccatg cgggcattca ggtaaggatc ttcaccataa ctttcccact ggcgtgacca
     3121 acgggcatca cggccgaggt ccatagtagg attaaatgtc cagggaatat tgctggcacg
     3181 tgtttcgtaa gccgaaattc ttgagccttc ttcggtcaat gcagtattga aagttgcggc
     3241 cattccaatt tcctgtggaa aaaatgtacc gccggcagta taggtcgttc cgtggttgga
     3301 gtcaattccg tagataaccg gaattccggt ctctttgata gctctttcct gaatggtgcg
     3361 gacaatgtat tcccaaactt cgggagtgcg ggcacggttg tttgcagtat tgaggatgga
     3421 tccaactttg tattttccaa taacggtatc gagcatagcc tgatcaatct ggaaaggttc
     3481 gtcacttgaa aaaacgtttg gtcctttgcc gattacgtcg agcgtaaatt gtgccatctg
     3541 accaactttt tcttcgagcg tcatatccgc catgattttg tcaattttct tctcgatgga
     3601 ggaatccagg ttttctcccg atttcttttg attacagccg ataagcacaa aaacagccgc
     3661 cagcagtaaa attccaattt tcttgttcat aatgaattgt tttttttcgt gtttttatag
     3721 atattttttt ttgttttagc attgaaaaat tgaaacccta tttttggtaa actctcacgt
     3781 agtcaatctc ataagtggca ggaagagcag caggatctat cccctgcgca ccgccccagt
     3841 taccacccca ggccatgttt aatttcaggt aaaatggtgc attgaagggc caggtagctt
     3901 tgttgttttg attgtcattg gaaaatttga aatactcttt tccgtcagca aatcctttga
     3961 tataagttgg agtccattcc agggaatata catgaaattc ggtggatgca tttgacacag
     4021 aggttttgtt ccctttttga gtcccgatgc tgtggttata agcagcagtg tgaacggtgg
     4081 aatgtacaac atttggttca taacctacaa attccataat atcaatttca ccgtcatccg
     4141 gccatttatt gaagtttttc ggcatcatcc agaatgcagg ccaggtgcct tttcccacgg
     4201 gcattttcat acgtgcctca aagtatccgt atagccagct gtctttggaa ttcattcgga
     4261 tggaaatgac ttcggaagct ctcttttttg cgatgatttt taaagttccg ttagaaataa
     4321 ccgcactcgt atctgttccg taaaatccgg gaacataatt ttgaatttcg ttgtttcccc
     4381 atccgcttgc acccgtttca taccaccaga ttgactcttt tggaagcgga tttttgccat
     4441 tacccagttt tgtatcgttg aattcgtccc accaaaccag gttatatcct gtaggaacaa
     4501 atccactgtg ctcacctgtg ccaccggttt gtccggttcc ggcagcttgt tttactgaaa
     4561 atgtcttagt agtttctttc gcagtgaatg tgagtgttgc ggttctcact ttgtcggtag
     4621 gatttttccc gactattacg ttgaaaaacg aaccggctgg aaatccgccg gaagctgaag
     4681 taatgcacca gttctgatcg gaagtaactt tccacgccgt attggtggtg atggtgatct
     4741 tttgtgactc gccgtcagcc ggaaaatcag cgagagtttc gggtgaaaat gtccaaacta
     4801 caggcgtgtt gggtttaggt tcatcagtgc cggttccatt tttactgcca cagttagaca
     4861 gcagtagcaa tgttaagatt aaaaagaaat tcatattttt catgcgtaaa taattattaa
     4921 atgagcccac caacttcgga ctgttgagtg ctgtttgtca gagtttttgt atcatttgcc
     4981 acgactttca agtcgtggat agttgaatat cctaagtatt ggctttagcc aaatttgttc
     5041 caaatataat gaaataaaaa tagttttgga ctaaaacccg atgttgaaag taactttatc
     5101 cacgactttt taaagttgag gtaataaaaa atgcagtttt cccttcgggg gatctgcctg
     5161 cggccccgac aggtcgggat aaaccaggca ggtttatggg gcttcccgcc atacgcttgt
     5221 gacggaatat tgcgggaaag accgcaatca agcagtcttt ccctgaagaa ttaattattc
     5281 agaaaaagta aaatcgtcaa agaagaagaa gccgggtccg gcgtgacctt ctgctccaaa
     5341 ctgaattacg attctgtcgt agtctgttct ggaagctaca ccactgaaat cataggtcaa
     5401 ttcaatccat ttgtctttta ccaaatctgc ttttacaatc tcagtttgtg tttcccacgg
     5461 tgcggggtgt tcgctgtttt ggagtttaat ggctaattgc ggttgcaatt ttgcatttga
     5521 aatccaggaa cctgccgttg catagtcagt tgtataatcg ttgtacgaag gaataaacac
     5581 tttaatcttg attttatttt gttttgtcag gtcaaattta tagtcagctg ccgtaaaacg
     5641 catattacta tagaattcag caggtttctg gtagcggtaa actttatttg aagtgttcag
     5701 aggtactgga agcggattgt caactacagc actcttatcg cccatggcat cgtgagtcaa
     5761 aataactttt ggagttgcac cctcaaagtc ttcagacaat ggaacagctt ttggttcttt
     5821 cactacgggt ggcggtggta cgttcagttc ctgaagggta taaacaaaca cccagtcctg
     5881 accttcaacg gtattattta cccgaagagc catcactttg tctgtctgat agaatacctc
     5941 gtattcttgc gaaccgcagt agtatcccat aaaggcgttt cctgatagtt tcagaattgg
     6001 attagctccg gtttcgtcga tagagaaagt gtaagctccg ccgttgtatg ggaagaatac
     6061 gtcatcaccg ttttctccgg taacagtaaa gccaccaaca cttgccgaac ttgctttacg
     6121 tccatatcct tcgccaccat tggtaatgtt aagttttaca ccggcttgga tgaaattaaa
     6181 tttgaaatca tacatcttcc atgaaccttt ttcattcgga cctgctcccc accattcctg
     6241 gccaagagat ccttgtggtc ctaagcccat gtgccctttc acgttctttc ctgttgcgtc
     6301 agctacttct ttcgcaaagt tgttgtattg gtcaaaaacc cacacctttc cggcaggatt
     6361 ttccatgccg cccgtgaggt tgatataagc ttcggaacta accagactga aatcgctgtt
     6421 tgcaattttc aacgtggaat tttttgaaac agctgttccg tcagggttgt agattgtaag
     6481 cataaccgga taatcaccgg cttcaggcag cgtaagttca attttatctt tgaagaaagt
     6541 ttgtaaatcg ccttttacat ccactgtcca aaccacagaa ataacgcctt tgtgaggtgt
     6601 ggtattggtc agtgttacga tgttcgggct ttttgcagat ggagtgatgg taaaagaaac
     6661 gtcttcagcc tttacgggcg tcatatctcc cagcgcatat gaagcatctt caaccggatt
     6721 acaagcggca aaaaatagca ccgccatgag catccacgat agatttttaa ttattttttt
     6781 cataaagtag tgtattaata tggattttgt tcaagtgcaa attcagtacc ggctgttttc
     6841 tcaatttcca actgaggaat cggcaagtat ttcttattaa tagtccaggt tctggagctc
     6901 tcggtaagag attcggtaag tacagaaggt gcatcgcccc atcttactac
//
LOCUS       DAORKP010000117         6708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2101_length_6708_cov_8.964227, whole genome shotgun sequence.
ACCESSION   DAORKP010000117 DAORKP010000000
VERSION     DAORKP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2101_length_6708_cov_8.964227"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLN75_07150"
     CDS             <1..211
                     /locus_tag="PLN75_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRG03930.1"
                     /translation="KAQSDSFKLDKEAVKAARENDYGIVIISASRDYGLDDLLFGPKE
                     YHLIRRSDVPLLLVNPRGDLYTLCD"
     gene            295..612
                     /locus_tag="PLN75_07155"
     CDS             295..612
                     /locus_tag="PLN75_07155"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRG03931.1"
                     /translation="MSEIKNKGSKLRRIVLNKYVLVLVVFLVFILFFDQHNLINRWKT
                     GRNIRSLEKEIRFYNDEIEMNKKKMNDMQVSDESLEKYAREQYYLKKDSEDIFIIKET
                     NEK"
     gene            602..940
                     /locus_tag="PLN75_07160"
     CDS             602..940
                     /locus_tag="PLN75_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03932.1"
                     /translation="MKNKSLHSVQLAGSLLTLTGAALQLFELEFSKYVFAIGALILIV
                     VYFIYTLRAKNEPNRIQRQHRLMLFATLFLGVGAYLMYTNKDTWVVMVLIYALISVFL
                     SFRKVEKQTE"
     gene            complement(983..2203)
                     /gene="serB"
                     /locus_tag="PLN75_07165"
     CDS             complement(983..2203)
                     /gene="serB"
                     /locus_tag="PLN75_07165"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRG03933.1"
                     /translation="MNSEIILINISGEDKPGMTNAITSILGKYNATILDIGQSDIHHN
                     LALGILFRTLDNQHSGNMLKEILFKCSELNMNVRFKPVDEKNYEQWVARQGKSRFIVT
                     VLGKVISAGQLSQVTGELAAQGLNIDSIKRLTGRPALDGNENVRSCIELSVRGDLKDK
                     AALSEQFMNLTVKEGIDISFQKDDIFRRNRRLICFDMDSTLIKTEVIDELADRAGVGD
                     EVRAITEAAMRGEIDFTESFSRRVALLKGLDVSVMKEIAENLPIMDGAERLMSVLKMC
                     GYKIAILSGGFTYFGNYLKKKFDMDYVYANELEVEDGKLTGRYVGDVVDGKRKAELLR
                     LISQVEKIQLEQVIAVGDGANDLPMLSIAGLGIAFHAKPKVKEVARQSISTIGLDGIL
                     YFLGFRDIHIDKDV"
     gene            complement(2178..3755)
                     /locus_tag="PLN75_07170"
     CDS             complement(2178..3755)
                     /locus_tag="PLN75_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRG03934.1"
                     /translation="MSKKNGFIVLAVCFLLIGCINRAAEIDRAPLAKPKPLSNDTLRV
                     VTEYDATSYFEYNGEPMGYDYELALELAKHLNLPMKLEVVKTDEEIIHMLRYGQAHIS
                     IFNFTETNDLKREFNMLFSEDESYMVLVQLVKRDAITDITELAGNEVWVKQNSIYHKR
                     LQTLNDEIGGGIIIRFAPDSVSTDDLMLQVAEKTIPYTLAYFNKAMLQKKFSRQLDCR
                     IPVGFNQRRGWLIPKSNRALTDSVNRWLNIESTKKYKSRLHNIYWLNNPYFAFKKVPI
                     PKGAISPYDDLFKLHAARIDWDWRLLAAVAYAESGFDSTAVSWAGARGVMQLMPGTAI
                     MYGVDSFDIENPAKNIAAATEYIKYLDLMYQNIEDREERIKFILASYNAGPARVLDAM
                     ALTRRYGKNPHLWFENTEFYMEKMDEAEFYKDSVVRYGPFGGTETLRYVPYVLDTYTR
                     FVEKGLTKAEKEKLLEKNKKTEKDSVVDKEKTREKDTLKNTEQEKKTVPEKETEPDKN
                     IELEKETENNEQRNHSD"
     gene            complement(3766..4500)
                     /locus_tag="PLN75_07175"
     CDS             complement(3766..4500)
                     /locus_tag="PLN75_07175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRG03935.1"
                     /translation="MDESLQKIMFIDIETVPQTAVFDELPEEMKHLWEDKYSLLQRKN
                     PEKYGTETTAAEGYFTNAGIFAEFGRIVCISVGYIFYRSNVMHFRTKSFAGKDEKTLL
                     SDFSELIIKNFPSRYDYFCGHNIKEFDIPYICRRMLIHRVPLPNALQLNGKKPWEVNL
                     LDTLDFWKFGDYKNFASLKLLTAVFGIPTPKDDIDGSQVARVFYLEDDLPRITAYCQK
                     DVQATAQVWLRMNGLETFNPENIEYA"
     gene            complement(4513..5127)
                     /gene="udk"
                     /locus_tag="PLN75_07180"
     CDS             complement(4513..5127)
                     /gene="udk"
                     /locus_tag="PLN75_07180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRG03936.1"
                     /translation="MLVIGIAGGTGSGKTTVVRKILERLPQGEVVILPQDSYYKDSSH
                     LPLEERLEINFDHPESLEWDLLIKHLKELKKGHDINQPIYSYLTCTRASETIPVKPTD
                     VIIVEGILILYNAELRKLLDLKVFVDADADDRLIRVINRDIVERGRSVNKVMERYELT
                     VKPMHLQFIEPSKRFADLIIPQGGNNHIAIDILTKYIENNLNNK"
     gene            5387..5575
                     /locus_tag="PLN75_07185"
     CDS             5387..5575
                     /locus_tag="PLN75_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRG03937.1"
                     /translation="MEKELKITVIKSGPLKVEGELKLIMPDGEEKVQNNAFLCRCGGS
                     SKKPFCDGTHKKIGFETD"
     gene            5728..6534
                     /locus_tag="PLN75_07190"
     CDS             5728..6534
                     /locus_tag="PLN75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRG03938.1"
                     /translation="MDRLRFRSLASGSSGNCYFLGNTSYGILIDAGVGVRNTRKFLKT
                     MGLDYCHIWGIFVTHDHADHIKAVGAIGEKFHVPVYATRLVHEGIDRNYCVTQKLSSS
                     RRFIETGDETVIGDFRVTAFPVSHDSTDSVGYSVQYKGFTFTFATDLGYVSDEAARYL
                     VNSDCVVLEANYDEFMLENGPYPLVLQTRIKADTGHLCNMQAATFLAENYTERWKYVF
                     LCHLSRENNMPELAFSTITGHLKDQMIPYEGRTEIVPLERTEPSRMYIFD"
BASE COUNT         1710 a         1479 c         1505 g         2014 t
ORIGIN      
        1 aaaagcccaa tccgacagtt tcaagctcga taaggaagct gtgaaagctg cccgcgaaaa
       61 tgattatgga attgtaataa tttctgcatc aagagattac ggacttgacg accttctgtt
      121 cggcccaaag gaatatcacc tcattcgccg atctgatgtt ccactgctgc ttgtcaatcc
      181 gcggggtgac ctgtacaccc tttgcgattg aaaaaatctg tttttcagat tagcaacctt
      241 actttcactt ccggaaaaat tgtctatctt tgcacaaatt acaccccgga gaacatgtct
      301 gaaatcaaaa acaaaggatc aaaactgcgt cgtatcgtgc tgaataagta cgtactggtg
      361 cttgttgttt ttctggtttt tatcctgttt ttcgatcagc acaacctcat caaccgttgg
      421 aaaacaggta gaaatatcag gagtctggaa aaagaaatcc ggttttacaa cgacgaaata
      481 gagatgaaca agaaaaaaat gaacgacatg caggtaagcg atgaaagtct ggaaaaatac
      541 gcccgcgaac agtattacct gaagaaagac agcgaggata ttttcataat taaagaaaca
      601 aatgaaaaat aaatcattac attcagttca gttggcaggt tcattactca cgctgacagg
      661 tgcagcgctc caactgttcg aactggagtt ttcaaaatac gtttttgcca tcggagcgct
      721 aattcttatt gtggtgtatt ttatttacac cctccgggca aaaaacgaac cgaaccgcat
      781 acagcgacag caccggctga tgctctttgc cacgcttttt ctgggagtag gagcgtacct
      841 gatgtacacc aacaaagata cctgggtggt aatggtgcta atttatgcgc tgatttcagt
      901 ttttctttcc ttccgcaaag tggaaaaaca gaccgaataa tcaaatcatt ttttttgttt
      961 tgggagtaga aacgctgctt ttttacacat ccttgtcgat gtgaatatcc ctgaaaccga
     1021 gaaaatacaa aatcccatcc agaccaatgg ttgaaatgga ctgacgggct acttccttca
     1081 ccttgggttt ggcatggaag gcaattccga gtccggcaat gctcagcata ggcagatcgt
     1141 tggctccgtc gcccacggca atcacctgtt caagttgaat tttctccacc tgcgatatca
     1201 gtctcagcag ttctgccttt cgtttcccgt ccaccacatc gcccacgtag cgtccggtga
     1261 gtttaccgtc ttccacttcc agttcgttgg catatacgta gtccatgtcg aattttttct
     1321 tcaggtaatt gccgaaatag gtaaatccac ccgagaggat ggcaatttta tacccgcaca
     1381 ttttcagtac cgacatcagt cgttcggctc cgtccatgat gggcagattt tcggcaattt
     1441 ctttcatcac gcttacgtcc agccctttga gcagcgctac gcggcgcgaa aaactttcgg
     1501 taaaatctat ttctccccgc atggcggctt cggtaatagc tctcacttcg tcacccacgc
     1561 ctgcgcggtc agccagttcg tcgattactt cggtcttgat aagtgtagaa tccatatcaa
     1621 aacagattaa ccggcggttg cgacggaaaa tatcgtcttt ctgaaacgaa atatcaatgc
     1681 cttctttcac agtgaggttc ataaattgct ccgaaagtgc cgctttatct ttcagatcgc
     1741 ctcttaccga aagttcgata caagatctta cattttcgtt tccatccagc gccggacgtc
     1801 ctgtgaggcg tttgatggag tcgatgttca gtccctgcgc tgccagttct ccggtgactt
     1861 gcgatagctg accggccgaa ataactttac cgagaacggt gacgatgaaa cggcttttgc
     1921 cctgccgggc tacccattgc tcgtagtttt tttcgtccac cggtttgaac ctcacgttca
     1981 tgttcagttc ggagcatttg aagaggattt ctttcagcat attgcccgaa tgctgattat
     2041 ccagtgttct gaaaagtatt ccaagtgcca ggttgtgatg aatatccgac tgaccgatat
     2101 ccagaatagt agcattgtat ttccccagaa tactcgtaat ggcattcgtc atgccgggtt
     2161 tatcttcacc tgaaatatta atcagaatga tttcgctgtt cattgttttc tgtctctttt
     2221 tctaattcaa tatttttatc cggttctgtt tctttctccg gtactgtctt tttttcctgt
     2281 tcagtatttt tgagagtgtc tttttccctg gttttttctt tgtcaaccac cgaatctttt
     2341 tctgtttttt tgttcttctc taaaagtttt tctttctcag cttttgttaa tcctttttct
     2401 acaaaacggg tataagtatc gagtacgtag ggcacgtaac gcagtgtttc agtgccgccg
     2461 aaaggtccgt atctgaccac cgaatcttta taaaattccg cttcatccat tttttccatg
     2521 tagaattcgg tattttcaaa ccagagatgt ggattcttac cgtatcgacg ggtgagagcc
     2581 attgcgtcca gtacgcgtgc aggtccggcg ttgtagcttg ccagaataaa ttttatccgc
     2641 tcttccctgt cttcaatatt ctgatacatc aggtccaggt atttgatgta ttcggtggct
     2701 gcagcaatgt ttttagccgg attttcaata tcaaaactat ctacaccgta catgattgcg
     2761 gttccgggca tgagctgcat gacgcctctg gcgcccgccc acgatactgc cgtgctgtcg
     2821 aaacccgatt cggcatacgc cactgctgcc agcagccgcc agtcccagtc aattcgtgcg
     2881 gcgtggagtt tgaacaaatc gtcgtagggc gaaatggctc ctttgggtat cgggactttt
     2941 ttgaatgcaa aatacggatt attcaaccag tagatattgt gcagtctgga tttgtatttt
     3001 ttggtgcttt cgatattcaa ccaacgattt acgctgtctg tgagtgcccg gttgcttttc
     3061 ggtatgagcc atcctcttcg ctggttgaaa ccaacgggta ttctgcagtc cagctgtcgg
     3121 ctgaattttt tttgaagcat tgctttgtta aagtaagcga gtgtgtaagg gattgttttc
     3181 tcagctacct gaagcatcag gtcgtcggtg cttaccgagt cgggggcaaa gcggataatg
     3241 atgccgcctc cgatttcatc gttcagtgtc tgcaggcgtt tgtggtaaat gctgttttgt
     3301 ttcacccaaa cttcattgcc tgccagttcg gtaatgtctg tgatagcatc ccgttttacc
     3361 agttgcacca gcaccatgta cgattcatct tccgaaaaca gcatgttaaa ttcccgtttc
     3421 aaatcgttgg tttctgtgaa gttaaaaatg gaaatgtgtg cctggccgta tcggagcatg
     3481 tgaattattt cctcatcggt ctttaccact tcgagtttca tcggcaggtt caggtgtttt
     3541 gccagctcca gtgccagctc gtagtcatat cccatcggtt cgccgttgta ttcaaagtac
     3601 gacgtggcat catattcagt aactactctc agcgtatcgt tggagagtgg ttttggcttt
     3661 gccaggggtg ctctgtcaat ttcggctgcc cggttgatac acccgatgag caggaaacag
     3721 actgccagta ctatgaatcc gtttttttta ctcattttga gttttttatg cgtattcgat
     3781 gttttcggga ttaaatgttt ccagtccgtt cattctgagc caaacctgtg cggtagcctg
     3841 tacgtctttc tggcagtaag cggttattct gggtaaatca tcttccagat aaaaaacacg
     3901 cgctacctga ctgccgtcaa tgtcatcttt gggtgtggga atgccaaaaa cggccgtcag
     3961 caatttcagt gaagcaaagt ttttataatc tccgaatttc caaaagtcca gtgtgtccag
     4021 cagatttact tcccagggct ttttgccatt aagctgaagg gcgttgggca gcggcacgcg
     4081 atggataagc atgcgccggc agatgtacgg aatgtcaaac tctttgatgt tatgaccgca
     4141 aaaataatca taacgtgaag ggaaattttt gatgataagt tcgctgaaat cactcaacag
     4201 tgtcttctcg tcttttccgg caaacgattt ggtgcggaaa tgcattacat tgctgcggta
     4261 aaaaatgtat ccgactgaaa tgcagactat gcgtccgaat tcggcaaaaa tcccggcgtt
     4321 ggtgaagtat ccttctgcgg ctgttgtttc agtaccgtat ttctccggat ttttccgctg
     4381 cagaagcgaa tatttatctt cccacaggtg tttcatctct tcaggcagtt catcgaaaac
     4441 ggctgtttgc ggaaccgttt cgatgtcgat aaacatgatt ttctgcagcg attcgtccat
     4501 agtggtggtg ctctatttgt tgttcagatt attctcgatg tatttggtca gtatatcgat
     4561 ggctatgtgg ttgtttcctc cctgcggaat gatcagatcg gcaaaccgct tggatggttc
     4621 gataaactgc aggtgcatgg gtttcacagt gagttcgtag cgttccatca ccttattcac
     4681 cgatcgtccg cgctccacga tatcgcggtt aatgacccgt atcagccggt cgtcggcatc
     4741 ggcatccaca aataccttca gatccagcag tttacgcagt tcggcattgt aaagtatcag
     4801 aattccctcc acgatgataa catctgtagg cttaacgggt attgtttcag acgcacgggt
     4861 gcaggtgagg taggagtaaa tgggttggtt tatatcgtga ccttttttga gttctttcag
     4921 gtgtttgatg agcagatccc actcgagcga ttcgggatgg tcgaaattga tttcgagccg
     4981 ttcttccagc ggaagatggc tgctgtcttt ataatacgaa tcctgtggga ggattactac
     5041 ttcgccctga ggcaggcgct ccagaatttt gcgtacgacg gtggtttttc ctgatccggt
     5101 gccaccggca attccaatta ctaacatgtt gtataatgat ttaatatttc agggcactaa
     5161 aataagaaat aatttccgaa ataaggataa agtaaaagat gttttcggaa tgaaaaaaaa
     5221 tcaggatgtt gcaattgaaa ccatcgttgg tgaaataacc tgattttacg cttttcagag
     5281 ctgttttttt gtcgtttgtt gtttcgatta ccttcgtttt atttttcgct gccgaaaaac
     5341 ctttacagct gctgatttgt tattatttta acaaaaaata attcacatgg aaaaagaact
     5401 gaaaattaca gtcatcaaaa gcggacccct caaagtggaa ggcgaattga aactgatcat
     5461 gcccgacggt gaagaaaagg tgcaaaataa tgcattcctg tgccgttgtg gcgggagtag
     5521 caagaaacca ttctgcgacg gaactcacaa aaaaatcggt ttcgaaacgg attaagtgct
     5581 tcaggaaatg aaaaacagat agggagatca ttctttatct gttttttgct tttacattca
     5641 tctgcatttt taacgttaat tacagggctt atgtggtaag tattcccttt tttatgttaa
     5701 ttttgtagct aattagtaag tgattgaatg gacaggttac gtttcagaag tttggcaagc
     5761 ggcagcagcg ggaattgtta ttttttagga aatacatcgt acggtatttt gatagatgcc
     5821 ggtgtgggag tgcgaaatac gcgtaaattt ctgaaaacga tggggctgga ttactgccat
     5881 atctggggca tttttgtaac acatgatcat gcggatcata taaaagccgt gggagcaatc
     5941 ggtgaaaaat ttcatgtgcc ggtatacgct acccgtctgg ttcacgaagg aatagaccgg
     6001 aattactgcg ttacgcaaaa gctgtcttca tcacggcgct ttatcgagac cggcgacgaa
     6061 acggtcatcg gcgatttcag ggtaacggca tttcccgttt cgcacgattc gaccgacagc
     6121 gtgggttact ccgtgcaata caagggtttt acctttactt ttgctaccga tttgggttat
     6181 gtgagcgatg aagcagcccg ctatctggtc aattcggatt gtgtggtgct cgaagcaaat
     6241 tacgatgaat tcatgcttga aaacggacct tatccgctcg tattgcaaac cagaatcaag
     6301 gctgataccg gacatctgtg caacatgcag gctgccacat ttctggccga aaactacacc
     6361 gaacgatgga aatatgtctt tctgtgtcat ttgagccgcg aaaacaacat gcccgaactg
     6421 gcgttttcaa ccattaccgg tcatctgaag gatcagatga ttccatacga aggaagaacc
     6481 gaaattgtgc cgctggaacg tacggaacct tcacgaatgt atatttttga ttgaaaaaaa
     6541 taattaaaag agttatgagt attgagttat gagtattgag ttatgagtat tgagtattga
     6601 gtattgagtg caaacccgaa acccgaaacc cgaaacccga aacccaaaac tcaaaacccg
     6661 aaacccgaaa cccgaaaccc gaaacccgaa acccgaaacc cgaaaccc
//
LOCUS       DAORKP010000118         6468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2270_length_6468_cov_14.594262, whole genome shotgun sequence.
ACCESSION   DAORKP010000118 DAORKP010000000
VERSION     DAORKP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_6468_cov_14.594262"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLN75_07195"
     CDS             complement(<1..622)
                     /locus_tag="PLN75_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRG03939.1"
                     /translation="MLLNIKDPLKDAAFIVATNIGVWSFDRFVLQAEYARINFKTVRN
                     NFKSGFVWDNDMFSTNLLMHPYHGGLYFNAARSNGKNFWQSVPFAAGGSLMWELFMEN
                     ESPSINDFMATSLGGAALGEMTYRVSDLLIDDRLTGFDRFKREALLTVISPVRGLNRI
                     INGDAWKHRNIRGNSVPATPVVFSVSAGHRFIADRLYKRQDYSNMLS"
     gene            complement(858..1619)
                     /locus_tag="PLN75_07200"
     CDS             complement(858..1619)
                     /locus_tag="PLN75_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRG03940.1"
                     /translation="MKAVIVEDELIAAQNLQRVIGQVAADIQVIAVLQSVEESVEWFS
                     LNPAPDLVFMDIHLADGSSFGIFEKVQIKSPVIFTTAYDEYALKAFEVNSIDYLLKPI
                     NVRNFERAISKYRNLVLAKDTNVDAISNLLATFNKTKDTWKSHFLIPYKDKLIPLAID
                     RIACFYVENKMAKIITTDDVIYYLDSPLEDLYAQLDPSEFFRANRQYIISHKAIKDIS
                     IWFGSKLSVNLIVAVPEKIIVSKARVADFKAWYTK"
     gene            complement(1616..2674)
                     /locus_tag="PLN75_07205"
     CDS             complement(1616..2674)
                     /locus_tag="PLN75_07205"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRG03941.1"
                     /translation="MRHKPLHRFSNALALRLSITLSIFLDCVLLLGYVYGNRIGESIG
                     LRLIYAFLSDVVLFYSLYSFTFKIIQLDIKKNAKFSYIILGSFAIVAVLNSIFTEGSI
                     RFFPSSLISANFLIVGNIMKDLIVMFIVLLSTMLLHSNIQRQQILLENEKLVADNIRI
                     RYEALKNQLDPHFLFNSLNTLDGLIGNDDEKAHEYVQNLSQVFRYAIGNKEITHLSDE
                     LDFTKSYALLMKIRYGDNLQIQYNIDEKYRNYFIVPVSLQLLVENAIKHNVISSRHPL
                     QITIETTLHDTIRVLNAIQPKSGVEHGEGIGLANLTERYEILFQKEVIITKTDVFCVE
                     IPLIKQQSDNTIKNPGLK"
     gene            complement(2800..5928)
                     /locus_tag="PLN75_07210"
     CDS             complement(2800..5928)
                     /locus_tag="PLN75_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRG03942.1"
                     /translation="MSIVETSIKKPLIVGVIFTLLTIGGILSYMNLNLNLMPKFELPM
                     LTVQTVYPGAGASEVETSVTKRIEDALSTLENLKKISSTSMEGVSVIAIELNAGADAN
                     LAVQEAQRKISAIKSNLPTDVKEPSISKIALEEKSILSVAASSSMPSTEFYKLVEDRI
                     QPRLAKLPGVGAVKLTGGKERQIRVNIDAEKLKAYKLSALQVLSAIQTANMEIPAGNV
                     ENSEQVYSVRLAAKYSSLNELRNTVIATTPNGGKVKVMDVAEVEDGVAEQKLINRIDG
                     KDAIGLSIQKQSDANAVKVADMAKAELKAIEKEYSANNVKFEIATDDSVYTNASAHSV
                     VIDLILAILIVAFVCFIFLHNIRSALIVMVAVPLSIIPAFIGMYLLGYSLNMMSLMAL
                     SLVVGILVDDSIVVIENMFTHMEKGKTRFRAAIDGCKQIMFTAMAITLVIVVVFLPLA
                     VSGGMIGNVLKEFATPIIIATMCSLLVSFTVTPLLMSRFGNIPDDTRPSLSGRFSRFV
                     ENAFESLKEWYAGILSWGLKHKVTILIVTVAIFIASLSLFPGGFIGFSFVPTTDQGEY
                     TVILDMNPQVTVYQNNQITMEAEKIINSIPEVQRVYTNVGMDSKTTKNNITTITVKMI
                     DKDKRKMSVQDCANLTKEKIMKQIPGVRVRASVASLGGGGQEPIQLIVQGTDYNQVQE
                     TAAMVLETVRHTPGTSDAKYSIDDPRQEVQIKLNREKMSALGLSVSDVGMTLRTALNG
                     NDNSKYTENNTEYKIRVGVDNFDRTKSEDVAKLTFINKKGELIELKQFADIYYGLGPS
                     ALERTDRISSITVKSMVVGRPSGTVGAEIEKAIEGKIPEGVSVKKSGMMEQQSTAFGS
                     LGFSFLAAIVLIYLIMVMLYNSLTDPLIVLFSIPLAMIGAFLALALTMHNLDIFSIIG
                     LIVLIGLVAKNAILLVDFTNKIRKEEGLNTFSALIEAGKDRMRPILMTTFAMIFGMLP
                     IALGAGDGAEMKNGMGWVIIGGLTSSMILTLVVVPVVYYIFDRIKFRKQVRRRKRLVK
                     KVTARQLERSLA"
     gene            complement(5951..>6468)
                     /locus_tag="PLN75_07215"
     CDS             complement(5951..>6468)
                     /locus_tag="PLN75_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRG03943.1"
                     /translation="LGAYVNPGSVIAGIADISQLKIVLEVSESNAYELYQGQQIDVTT
                     DVNPGARYAGKIANISPKASASHTYPVEILIPNTGKDKLKAGTYVNVSVNMGKSGKAL
                     MIPRDAIVSSVKDPSVYVVNDNAVKLVKITTGRSFNSNLEVAAGLNEGDKVVTNGQIN
                     LMDGAKVSILN"
BASE COUNT         1801 a         1438 c         1328 g         1901 t
ORIGIN      
        1 agctaagcat attgctgtaa tcctgtcgtt tgtacagacg gtcggcaatg aagcgatgcc
       61 cagccgatac ggaaaaaacc acgggagtgg ctggcacgga attcccgcgg atattacgat
      121 gcttccaggc gtcaccgttt ataattctgt tcaatcccct gaccggtgag atgacggtca
      181 gtaaagcttc gcgtttaaaa cgatcaaaac ccgttaaccg gtcgtcgatt agcagatcgg
      241 acaccctgta ggtcatttca cccagagccg cgccacccag agaggtagcc ataaaatcat
      301 taatggaagg agactcattt tccatgaata attcccacat caggctgcct cctgctgcaa
      361 aaggaaccga ttgccagaaa tttttcccgt ttgaacgtgc cgcgttgaag tataatccac
      421 catgataggg atgcataaga agattggtag agaacatgtc attgtcccat acgaagccgg
      481 atttaaaatt gttcctcaca gttttgaagt ttatccgggc atactcagcc tgaaggacaa
      541 atcgatcgaa ggaccaaaca ccgatattgg tggcaacaat aaaagcggca tctttcagtg
      601 gatcttttat atttaaaagc aatgaatcgc tacgatttgt tcctgagatt gaacctgtat
      661 ggtcgggaac ctggctttgg ccgtggaata tcagaaaaca aaaaatcagt gtcagtaatg
      721 tcctgaaatg atacttattc ataaacaaca aaaaaaaagt agcaatcgac aaatttatta
      781 aaggagaaat atctgagttt ctgtgtctga aatttaaaga atgctgttat taaaaattga
      841 atataaaaaa aaacggtcta tttggtgtac caggccttga aatccgcgac gcgggcttta
      901 cttactatta ttttttcagg tacagcaaca atcaggttta ctgacagttt acttccaaac
      961 cagatagaaa tatctttgat tgctttgtgt gaaataatgt actgacgatt agccctgaaa
     1021 aactcagaag ggtcgagctg cgcgtacaaa tcttcaagcg gactgtccaa atagtaaatc
     1081 acatcatctg tagtgatgat ttttgccatt ttattttcaa cataaaaaca agctatcctg
     1141 tcaatggcca aaggaattaa tttatcttta tatggtatca gaaaatgtga tttccaggta
     1201 tctttcgtct tgttgaaagt agccagcaga ttgctgatcg cgtcaacatt tgtatcctta
     1261 gccaatacca gattacggta tttagaaatt gcccgttcaa agtttctgac attaattggt
     1321 ttgagcaaat agtcgatact gtttacctcg aaggctttca gggcatactc atcataagct
     1381 gtagtgaaaa taaccggact ttttatctga accttttcaa aaattccgaa cgaagaaccg
     1441 tcagccagat gaatatccat aaacaccagg tccggagcag gattgagtga aaaccactcc
     1501 acactttcct cgacactttg caatacggca atcacctgga tgtcggcagc tacctgacca
     1561 attacacgtt gcagattttg agcagcaatg agttcatctt ctacaataac agctttcatt
     1621 ttaatcccgg atttttaatt gtgttatctg attgttgttt aatcaatgga atttccacac
     1681 aaaaaacatc tgtttttgtt atgatgactt ctttctgaaa caggatttca tacctttcgg
     1741 tcaggttagc caggccaatt ccttcgccat gttccacacc gcttttgggc tgaatggcgt
     1801 tcagtactct tatggtgtca tgcagcgtgg tttcaatggt tatctgcaac ggatgcctgc
     1861 tgctgattac attgtgtttg atagcatttt ccaccagtaa ttgcaggctg accggaacga
     1921 taaagtaatt ccggtatttt tcgtcaatat tgtactgaat ttgcaaatta tcaccgtagc
     1981 gtattttcat caacagggca tacgatttgg taaaatccag ttcatcactc agatgcgtta
     2041 tttctttgtt gcctatagca tatcgaaata cctgcgataa attctgcaca tattcgtggg
     2101 ctttttcgtc gtcatttcct atcaatccat cgagcgtgtt cagcgagttg aacagaaaat
     2161 gaggatcaag ttgattttta agcgcttcgt aacgaattcg gatattatct gcaaccagtt
     2221 tttcattctc gagcaatatc tgttgacgtt gaatgttaga atgaagtaac attgttgata
     2281 ataacacgat aaacatcaca ataagatctt tcataatatt ccctacaatc agaaagttgg
     2341 ctgaaatcag acttgaggga aaaaaacgta tggaaccttc ggtaaaaata gaatttaata
     2401 cggcaacaat ggcaaatgaa cccagaatga tgtagctgaa ttttgcattt ttctttatgt
     2461 ccagctgtat tattttgaac gtgaagctat ataatgaata gaacaaaaca acatctgaca
     2521 gaaaagcata aatcagccgt aagccgattg attctcctat tctgttacca tatacatatc
     2581 ccagcagcaa aacacaatcc agaaatatac ttaatgttat tgatagtctg agagctaaag
     2641 catttgaaaa gcgatgtaag ggtttgtgtc tcatcgcaaa aatggtagag tttttaaaat
     2701 tgattggagt attgatttta ctcacaaatt atttttgacg ataaaaatac taaaaaacaa
     2761 cgactaaact ataaaagtcc ggtcgttgta tgtaatctat tacgctaaac ttctttccag
     2821 ttgcctggca gtcacttttt taaccagtct tttacggcga cgcacctgtt ttctgaattt
     2881 gatgcgatcg aaaatataat ataccacagg caccaccacc agtgtgagga tcatcgaact
     2941 cgtaagcccg ccgataatca cccaacccat accgtttttc atttcggctc cgtcaccggc
     3001 tcccagcgca atgggcaaca taccgaagat catcgcgaag gtggtcatca gaattggacg
     3061 catacggtct tttccggctt caatcagggc actgaatgta ttcagtcctt cttccttgcg
     3121 tattttattg gtgaagtcaa ccagcaaaat cgcattcttg gccaccagac cgataagcac
     3181 tatcaaacca atgatactga agatatccag attgtgcatg gtcagcgcca atgccaggaa
     3241 agctccgatc atagccagcg ggatggaaaa caatacaatc agcgggtcgg tgagcgaatt
     3301 gtaaagcatg accatgatga gataaatcaa cacgatagcc gccaggaaag agaagcccaa
     3361 gctgccgaaa gcggttgatt gttgctccat catcccactc tttttgacgc tcacaccttc
     3421 cggtattttt ccttcaattg ctttttctat ttccgcaccc actgttcctg atggacgacc
     3481 gacaaccatt gattttacag taatggatga aatacgatcg gtacgttcaa gcgccgatgg
     3541 gcccagcccg tagtagatgt cagcaaattg tttcagttca atcagttcgc ctttcttgtt
     3601 gataaaggtg agtttggcta cgtcttccga cttggtacgg tcgaagttat cgaccccgac
     3661 acgtattttg tattctgtat tgttctctgt gtatttgctg ttgtcgttac cgttcagtgc
     3721 cgtacgcagg gtcattccca catcacttac cgacaatccc agagctgaca ttttttcacg
     3781 gttgagttta atctgcactt cctgacgcgg atcatcaatg gaatatttcg cgtccgaagt
     3841 tccgggtgta tgtcgcaccg tttccagtac catagctgct gtttcctgaa cctgattata
     3901 atcggttccc tgcacaatca gctggatagg ttcctgacca ccaccgccta agcttgccac
     3961 agaagcacgc acccggacac caggaatttg tttcatgatt ttttccttgg tgaggttggc
     4021 gcaatcctgt acgctcatct tacgcttgtc cttgtctatc atcttcaccg tgatggttgt
     4081 aatgttgttt ttcgtcgttt tggagtccat gcccacattg gtatatactc tctgaacttc
     4141 ggggatgctg ttaatgattt tttcagcttc catggtaatc tggttgttct gataaactgt
     4201 aacctgcggg ttcatatcca gtataacagt atattcgccc tggtctgtgg tcggaacaaa
     4261 ggagaaacca ataaatccac cggggaacag tgacagggaa gcaatgaaga tggcaactgt
     4321 aactatcaga atggttactt tatgtttcaa tccccaggaa agaatcccgg cgtaccattc
     4381 cttcagtgat tcgaacgcgt tttccacaaa tcttgagaat cgtccggaaa gtgaaggacg
     4441 ggtgtcgtca ggaatattac cgaaccgtga catcagaagt ggagttaccg taaatgaaac
     4501 cagcaaactg cacatggtgg caataataat cggtgtggca aactctttca gaacgtttcc
     4561 tatcataccg ccactcactg ccagcggaag gaatacaacc acaatcacca gggtgatagc
     4621 catggcggta aacataatct gtttacaacc atcgatagct gcccgaaatc tggtcttccc
     4681 tttttccatg tgtgtaaaca tgttttcaat gaccacaatg gagtcgtcca caagaatacc
     4741 cactaccaat gataaggcca tcagcgacat catgttcagc gagtaaccca gcagatacat
     4801 gcctatgaag gccggtatta tagagagcgg aacagctacc attacgatca gcgcacttcg
     4861 tatattgtga aggaagataa agcaaacaaa tgctacaatg agaatagcca gtatcaagtc
     4921 aataaccacc gaatgtgccg aagcattggt gtacaccgag tcatcggtag caatttcaaa
     4981 ttttacgttg ttggccgaat attccttttc aatggctttc agctctgcct ttgccatatc
     5041 ggcaacctta acagcattgg catcactttg cttctgaatg gaaagtccga tggcgtcctt
     5101 accgtcgata cgattgatca gtttttgttc ggcaaccccg tcttcgactt cggcaacatc
     5161 catcactttc actttacctc cgttgggagt tgtagcaatc acggtgttgc gaagctcgtt
     5221 cagactggag tatttagcag ccaaacgtac cgaatagacc tgttctgaat tttccacatt
     5281 ccctgccgga atttccatat tggcagtctg aatggccgat aatacctgca aagccgaaag
     5341 tttatacgct ttcagtttct ctgcatcaat atttactctt atctgccgtt ccttaccacc
     5401 tgtgagtttt accgcaccaa cacccggaag ttttgccagg cgtggctgaa tacggtcttc
     5461 caccagtttg tagaattcgg tggaaggcat cgatgaggat gcagctacgc tcaggatgga
     5521 tttctcttcc agggcaattt tactgatgga aggttccttc acatcggtgg ggagattgga
     5581 tttgatagct gatattttac gttgagcttc ctgcacagcc aggttggcat cggctccggc
     5641 attcagttcg attgcgataa ccgacacgcc ttccatggag gtggagctta ttttcttcag
     5701 gttttcaagt gtcgaaagag catcttctat ccttttggta accgaagttt ccacttccga
     5761 tgctccggct ccgggataca ccgtttgaac cgtcagcata ggtagctcaa acttaggcat
     5821 taagttcaga ttgaggttca tgtaggacag gattcctccg atagtcagca gcgtaaagat
     5881 tacacccact atcaaaggct tttttattga ggtttctaca atagacatat cgttgttttg
     5941 gattaaaaat ttagtttagg atggatactt ttgctccgtc cattaagttg atctgaccat
     6001 tcgttaccac tttatcacct tcattcagac cagcagctac ttcgagattg gaattaaaac
     6061 tgcggccggt ggttattttc accagtttta ccgcgttatc atttacaacg taaaccgaag
     6121 gatctttcac actgctcaca atggcatcac gcggtatcat cagcgctttt ccactcttgc
     6181 ccatatttac acttacattt acatatgttc cggctttcag tttgtctttt ccggtgttag
     6241 ggatcagaat ttcaaccgga taggtgtgcg aagcgctcgc cttggggctg atattggcta
     6301 tttttcccgc atatctggct ccgggattta catcggtggt tacatcaatc tgttgtccct
     6361 gataaagttc gtatgcattc gattcggata cttcaagcac aattttcagt tgagaaatat
     6421 cagctattcc cgcaattacc gatccgggat tgacataagc tcctaatt
//
LOCUS       DAORKP010000119         6449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2282_length_6449_cov_7.544886, whole genome shotgun sequence.
ACCESSION   DAORKP010000119 DAORKP010000000
VERSION     DAORKP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2282_length_6449_cov_7.544886"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLN75_07220"
     CDS             <1..531
                     /locus_tag="PLN75_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03944.1"
                     /translation="TQSVLISYVGATKTVTTTCPLTLVKGIAQVGLTDMTTAWWSAFS
                     DDYNVASGTSKTITLYCYSDNAANYHSPCTILRKADKSEYAVVRMDNFGWGNGYNTAT
                     LTSDWNWDNFASSISGSRVAITVTNNGNNTADILYKVTYVNGESHFQKYAGVTVDSSD
                     LNCALVTEGSYLVIIK"
     gene            554..3751
                     /locus_tag="PLN75_07225"
     CDS             554..3751
                     /locus_tag="PLN75_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG03945.1"
                     /translation="MKYSYFILLGLLALTPATYAAGNINTAQMTMAQAGNITIKGKIV
                     DERKEPLPGATIQQKGTSNGVITDIDGNFSISASSDATLTVSFIGFRSVEIPVGGRTA
                     LGNITLISEIKELDQLVVIGYGSQRKVDLTGSVAVVNTEEMKKVSNSNISTMLEGKVA
                     GVQITSDGQPGADPTVRIRGIGSFGSTAPLYVIDGVPMGTTIRDFSPNDIETLQVLKD
                     ASAAAIYGSRAANGVIIITTKQGKKNEAMRINYSGYYGMDQVQNGVYNVMDAYQYGDY
                     VTMAYNNSGMDVPAGYNPASAKYIDPAQVNTNWFNEAFKTGIRQNHNINMSGGGTNNT
                     YNIGLDFYSQRGTMVGAGPNFDRYTARVNNTMDVKFVKIKTNIVYSHSNQDNMALSNA
                     NEYVQGLYGAQYPVMASALITPPTIKAYDPSTWVLDDKISAASEYSYDSYGYGTYYDD
                     VHGDLRVTNVLLTNSLLKRNSTVDRIVAAASATVDFIGMVGHKSKNHKLDYNLNVSYS
                     RTFAKDFTFVPAFIQSTTNYLSKSNENLSQGYRNYSDALVENTINYDGKFGLHHINAV
                     VGQTFERELYHTLTGKGVNMPEPYYLQVNNAEETDATSYESEHVLASYIGRINYDFNE
                     KYLISATARRDGSSRLSSNDRWDWFPSVSGGWRMEREEFFPIAESKINLFKLRGSYGV
                     LGNENIGEYQYMDVMSRGNYTYSFGNNKVTGSAISNYVNTAIRWEKKKTIDFGLDLGM
                     FSNQLEFTFDWYKSTSEDLLYSVAVPTNAGATNGTVTMNAATMQNSGLEFLLAYHNHK
                     NPVKFDISANLSTLNNKVIKLGVSGEPRTDGYSRTEVGREVGAFYGYVSEGLFQSQEQ
                     IDNRVNAEGEHINQNGAQPGDVIYADLNLDGEITNEDQQFLGSGLAKIHYGLNARAEW
                     KGFELSVSTYGAAGFKAVDFVDVTLHSSYGALNKSVDLLNAWTPNNTNTNVPRVAYKS
                     SGSITNDMFSQRFIQNASYLKIANIEVGYNFPDRWFGNYLNGVRIFASAQNLATLSEY
                     KGYNVDFSGGTFTPGYNYASYPTPRTIMFGAHFSF"
     gene            3784..5532
                     /locus_tag="PLN75_07230"
     CDS             3784..5532
                     /locus_tag="PLN75_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG03946.1"
                     /translation="MKNINRILIMLAFVGGMTACNDELDVQNPNNQTTYEFGNTESEL
                     QEAVIACYNRIRLEGTFARVGYTLDAVRGDEVWNSSQQWYLEYDNLNSPGTIEIGDMW
                     PWRDNYHIVNRTNFVLSKVDVVKMSETSYNQIAGQALFLRSLAYYNLATYYQNVPLIT
                     DYASYSNINTMYAANNTQDEVFDQIEADLTKAMQLLPSRDKGGEWAKGRATCGAAAGY
                     LARALMFRHKYAEAYVVLKDIIAGKYGHYELTADYGDNFRENTENNVESLFEVQFLDF
                     GTGGTDEEWTPVNISSQATQGHAVESNYASQALGSWGDLAGAPWLYNLFRKENSTDGR
                     LDPRLYWTLITYESEYSSYTGAKTAAYPNGDPRSNTIYQQDITATPLSNNAQGGISIA
                     KFTNARNNIYSSITNGLHCGINLRLMRYSDVLLRAAECENELHGPTQIAIDYINIVRR
                     RVALKDLKLSDFPSADALFEQIANVERPKEFGCENGRGIDLLRWGFFYSTDRLNQLVE
                     HGYYKLDGKASTDELTAETASGSSFKYYYKGHEYFPIYQSTLNANPNLVGNSANKNED
                     NAPAFTAKWTVRPVVK"
     gene            6041..>6449
                     /locus_tag="PLN75_07235"
     CDS             6041..>6449
                     /locus_tag="PLN75_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HRG03947.1"
                     /translation="MKLKYLLISLIGITTFSYVGCKDEAPQPDDPKPDTLANFSIPSY
                     ADDYSSISSWSNRNKWNLANVHDPSVAYYKGYYYMYGTDASYGNEAVGHGHFQGKRST
                     DLVNWNWVGGPFYQAPSWVADSLNAIRSRMGLVA"
BASE COUNT         1992 a         1491 c         1346 g         1620 t
ORIGIN      
        1 actcaatctg tcttaatctc ttatgtagga gcaacaaaaa cagtgactac cacctgcccg
       61 ttgacgcttg ttaaaggtat agcccaggta ggtttaaccg atatgacaac tgcctggtgg
      121 tcggcattct ccgacgacta caatgttgca tcaggcacga gcaaaacaat tactttgtac
      181 tgctactccg acaatgcagc caactatcac agtccctgta caattcttcg caaggccgac
      241 aagagtgaat atgcagtagt gcgaatggac aacttcggtt ggggtaacgg ctacaacact
      301 gccactctga caagcgattg gaactgggat aatttcgcat caagcataag cggatccagg
      361 gtagctatca ctgttacaaa caacggaaac aataccgccg atatacttta caaggttact
      421 tacgtcaatg gcgaatcaca tttccagaaa tatgcaggtg taacggtaga cagttctgat
      481 ttgaattgtg cactcgtaac agaaggatct tatctcgtaa ttattaaata atcacaaaaa
      541 aaatatttaa attatgaagt attcatattt cattctattg ggattgctgg cacttacacc
      601 ggcaacctac gcagcaggca acatcaatac tgcccagatg acaatggctc aggcaggcaa
      661 tataacaata aaaggtaaaa tcgttgacga aagaaaagaa ccgcttccgg gcgctaccat
      721 ccaacaaaaa ggcacatcca atggtgtcat taccgacatc gatggtaact tctcaatttc
      781 tgcctcttct gacgctacgc ttacagtctc ctttatcggc ttcagaagtg ttgaaatacc
      841 cgttggagga agaacagcac tcggcaatat cacgctgatt tcggaaataa aagaacttga
      901 tcaattagtt gttataggtt atggttcgca gcgcaaagtt gaccttacag gttcagttgc
      961 agttgtcaac accgaagaaa tgaagaaagt atccaattca aacatatcga caatgctcga
     1021 aggtaaagta gcaggcgttc agattacatc cgacggacag cccggagccg accctacagt
     1081 acgtattcgt ggtatcggtt cgttcggtag cactgctcct ctctacgtta tcgatggagt
     1141 gccaatggga acaacaatac gcgacttttc gcccaacgat atcgaaaccc ttcaggtact
     1201 taaggatgca tcggctgccg ccatctacgg ttcgcgtgca gccaacggcg tgattatcat
     1261 caccaccaaa cagggtaaga aaaacgaggc catgagaatc aactatagtg gctactatgg
     1321 catggatcag gtacaaaacg gggtatacaa tgttatggac gcttaccaat atggggatta
     1381 tgtaacaatg gcctacaata acagcggtat ggatgtacct gccggataca atcctgccag
     1441 tgcgaagtac attgaccctg ctcaggtaaa tacaaactgg ttcaacgaag cgttcaaaac
     1501 aggaattcgt cagaaccaca acatcaacat gtcaggaggc ggtaccaata atacttacaa
     1561 catcggtctc gatttctata gccaacgggg aacaatggta ggagccggtc caaatttcga
     1621 ccgctatact gctcgcgtca acaacactat ggacgttaag tttgtaaaaa tcaaaacaaa
     1681 tatcgtatac agccacagca atcaggacaa catggcgctc agtaatgcca acgaatacgt
     1741 acaaggtctg tacggagcac aataccctgt tatggcatcg gcgctgatta caccaccaac
     1801 catcaaggcc tacgatccat ccacctgggt actcgacgat aagatttcgg ctgcatctga
     1861 atacagctat gactcttacg gttatggtac ttactacgac gatgttcatg gtgacttacg
     1921 tgtaacaaac gtacttctca ccaatagctt gctcaaaagg aactcaactg tggaccgtat
     1981 tgttgcagca gcttctgcaa ctgtcgactt cataggcatg gttggccaca aaagcaaaaa
     2041 ccacaagttg gattacaatc tgaatgtatc gtacagccgg acttttgcaa aagactttac
     2101 atttgtgccc gcatttattc agagtacaac aaactatttg tcgaaaagca acgaaaacct
     2161 gtcacagggc tatcgcaact acagtgatgc acttgtagaa aacacgatca attacgatgg
     2221 taaattcgga ctccatcaca tcaatgctgt tgtgggtcaa acattcgaac gcgaactcta
     2281 ccatacactc acaggcaaag gcgtgaacat gcccgaacct tattacctgc aggtaaacaa
     2341 tgcagaggaa actgatgcaa ccagctacga aagcgaacac gtgttggcat catacatcgg
     2401 tcgtatcaat tacgatttca atgaaaaata ccttatttcg gcaacagcac gtcgtgatgg
     2461 ttcaagtcgt ctgtcctcaa atgaccgttg ggactggttt ccatctgtat caggaggctg
     2521 gcgcatggaa cgcgaagaat tcttccctat agccgaatcg aagatcaatt tattcaaatt
     2581 acgtggtagc tacggtgtac ttggaaacga gaacataggc gaataccaat acatggatgt
     2641 aatgtcgaga ggcaactaca cctatagctt tggtaacaac aaggtgaccg gctcagccat
     2701 atcgaactat gtgaacacag ccattagatg ggaaaagaaa aaaacaattg actttggtct
     2761 tgatctcggt atgttcagca atcaattgga atttactttc gactggtaca aatctacatc
     2821 cgaagacctt ctttacagtg tagctgtacc aacaaatgca ggcgctacca acggaacagt
     2881 aacaatgaat gcagcaacaa tgcaaaactc aggactagag tttttgcttg cctatcacaa
     2941 tcataaaaac cctgttaagt tcgacatttc ggccaactta agcactctca acaacaaggt
     3001 aataaagtta ggcgtttcgg gcgaaccccg taccgatggc tacagccgta cagaggtagg
     3061 tcgtgaagta ggtgcattct atggttatgt ttccgaagga ctcttccagt cgcaggaaca
     3121 gatagacaac cgcgtgaatg cagaaggcga acacatcaat caaaacggag cacaaccggg
     3181 agatgttatc tacgccgacc tcaacctcga tggcgaaatt acgaacgaag accagcaatt
     3241 cctgggcagt ggtttagcaa aaatccatta cggattgaat gcgcgggccg aatggaaagg
     3301 tttcgagtta agcgtctcta cctatggagc tgcaggcttc aaagcagttg actttgttga
     3361 tgtcactttg cacagctctt atggcgcgct caacaagagc gttgacctgc tgaatgcatg
     3421 gacacctaac aacacaaaca ccaatgtgcc ccgcgtagct tacaaatcgt caggttcaat
     3481 cacaaacgat atgttcagtc agcgctttat tcaaaatgca tcctatctta aaattgccaa
     3541 tatcgaagtg ggctataact tccccgacag atggtttggc aattatctga atggcgttcg
     3601 catatttgca tcggctcaaa atctggccac tttgtctgaa tacaaaggtt acaacgtaga
     3661 cttctcggga ggtacattta cacccggata caactatgct tcgtatccaa caccacgaac
     3721 cattatgttc ggagcacatt tctcattcta aaaaaataat tcaaacaaaa atagaatatc
     3781 aatatgaaaa atataaacag aattctaata atgttggcat ttgttggagg catgacagcc
     3841 tgtaatgacg aactcgatgt tcagaaccct aacaatcaga ccacttacga atttggcaat
     3901 accgaatcag aacttcagga ggctgttatc gcgtgctaca accgcatacg cctcgaaggc
     3961 acatttgccc gcgttggtta cactttggat gctgtacgtg gcgacgaagt gtggaactcg
     4021 tctcaacagt ggtacctgga gtacgacaac cttaattctc cgggcacaat agaaataggt
     4081 gacatgtggc catggcgcga caactaccat attgtaaacc gtaccaactt tgtattatcg
     4141 aaagtagatg ttgtaaaaat gtcggaaact tcttataatc agattgccgg acaagccctc
     4201 ttcctcagat cgcttgccta ttacaacctg gctacctact atcagaatgt tccgttaatt
     4261 accgactatg catcctattc gaacatcaac acaatgtatg cagccaacaa tacgcaggac
     4321 gaagtatttg atcagataga agctgatctc acaaaagcaa tgcagttatt gccatcgcgc
     4381 gacaaaggtg gcgaatgggc gaaagggcgt gctacatgtg gcgccgccgc cggatacctg
     4441 gctcgcgcac tgatgttccg tcataaatat gccgaagctt atgttgtact gaaagacatt
     4501 atcgccggca agtacggaca ttatgaactt acagccgact atggtgataa tttcagagaa
     4561 aatactgaaa ataatgttga gagccttttc gaagttcaat ttcttgattt tggcacaggg
     4621 ggcaccgacg aagagtggac tccggtaaat atcagctcgc aagccactca gggtcatgct
     4681 gtagaaagca actacgcttc gcaggcattg ggtagctggg gtgaccttgc cggcgcacca
     4741 tggctgtaca acctgttcag aaaagaaaac agcactgatg gccgtcttga tccacgttta
     4801 tactggacat tgattaccta cgaaagtgaa tacagcagct ataccggcgc taaaacagca
     4861 gcatatccta acggagatcc ccgcagcaat accatttatc aacaggatat tactgcaact
     4921 cctctatcga acaacgcaca gggcggtata tcaattgcta aatttacaaa tgcgagaaac
     4981 aatatctata gttctattac aaatggtttg cattgtggca tcaacctgcg tcttatgcgc
     5041 tacagcgatg ttttattgcg tgcagccgaa tgcgaaaatg agctccatgg ccccactcag
     5101 atagctatcg attacatcaa catcgtccgc cgccgcgttg cgcttaagga tcttaaactt
     5161 tccgacttcc catcggccga tgcgcttttc gaacaaatag caaacgttga acgtcctaaa
     5221 gaattcggtt gcgaaaacgg acggggtatt gacttactcc gttggggatt cttctacagt
     5281 accgatcgtc tgaatcaatt ggtagagcac ggttattaca aactcgatgg taaagcttcc
     5341 accgatgagc ttacagccga aactgcttca ggttcatctt tcaaatacta ttacaaagga
     5401 catgaatatt tccctatcta tcaatcgacg ctgaatgcaa atccgaacct tgtaggtaac
     5461 tcggccaaca agaatgaaga caatgcacct gctttcacag ctaaatggac ggttcgtcct
     5521 gttgtaaaat agaaattatt cgcctatatt tgaaacttcc ttatcaacta ttttttatgc
     5581 tgataaggaa gttttattta ccaaaaaatt cagacacgaa acgggcatta aagtatttac
     5641 tattttacaa tgtacaattt actattttaa tgcgaatcag gggttgaaaa taattctatt
     5701 ttgcttattg taaactattt cccataggga atgtatatcc atagaaaata gaaaatcatc
     5761 aaaatcagtt ccccgtaggg gataaataaa tataaaacat gaatgtccta cggacaataa
     5821 aacattgatg cgtgtctatt ctattgatat tgaagtccta tgaactactt tcaactcctg
     5881 atttgcatat ttaataaaga tagataaaca gcatgaaaca cccatgacct aactaaaaat
     5941 tcaggacaca caaggagatg attaaaattt cactaaacat tctttgtccc ttcttattcc
     6001 ccctttaggg ggctaggggg cagacataaa ttacaaacac atgaaactaa agtatttact
     6061 tatttctttg atcggcataa ctaccttctc ctacgtaggt tgcaaagacg aagcgccgca
     6121 gccggatgat cccaagcctg acacccttgc caatttcagt attccatcat acgccgacga
     6181 ttattcatcc atttcatcgt ggagcaaccg caacaagtgg aaccttgcca acgttcacga
     6241 cccatcggtg gcctactaca aaggctatta ctatatgtac ggcaccgatg cctcctatgg
     6301 caatgaggct gtaggacatg gacattttca gggcaaacgc tccaccgatc tggtcaattg
     6361 gaattgggta ggcggacctt tttatcaggc gccttcctgg gtagccgact cgctcaacgc
     6421 cattcgttcg cgcatgggac tcgttgcca
//
LOCUS       DAORKP010000120         6377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2350_length_6377_cov_8.379943, whole genome shotgun sequence.
ACCESSION   DAORKP010000120 DAORKP010000000
VERSION     DAORKP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2350_length_6377_cov_8.379943"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="PLN75_07240"
     CDS             complement(<1..2032)
                     /locus_tag="PLN75_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRG03948.1"
                     /translation="MKKQVLFLLLVLLTNTLVAQLKINELMTNNVSAVIDDSYNYSMW
                     VELYNTSTTTSYNQYSYYFTDDLTQPKKWHPPVSKMISPRGYSVLWFERSDRSGHASF
                     RLKPEGGVLYLLNESGQVIDNVMYPAQYRNISYGRVSDGSLEWTYFEQYSSGLTNNGK
                     KSATERCAKPVFVTKGGFYNTPIYVTLDSPMTGDTIYYTTNGAEPTRSHIRYTPGSVI
                     SISRTSIIRARCFSRNKLSSDIASCTYFIGERNFRLPVVSVVTEQKNLTDNTIGIYVQ
                     GTNGITGNGMNTPANWNQDWDRAANIELYDTTNVSRINQEVDIRIAGGWTRMNGQKSL
                     ILNPTKKYGDNKFDYDIFKATKPGMKYRSIMLRNSGNDFDHSMMRDGYMQSLVMKRMN
                     LDCIAYEPAVCFMNGVYYGIQNLRERSDEDFVYSNYGYDEEDIFLVESWEMDYDSEFK
                     KLTNYVSNSDITQKAVYDNVCTMMDMDNFMDYFLTEIYLRNTDWPHNNVKAWKKKDGG
                     KWRWILYDTDFGYNIWGNDHTHNTLIWALGEEAGSLPANAPWSTLLLRRLVLNETFRK
                     RFIDKFAIQISSTFEVNRANAILDSLAAKIRTEIVYHKNKWGQGRDFNTDISIMKGFS
                     ANRPTSMMNFIGARFMNNAGTAEIQISSNNPKASYKFNAETIQDAQINLKYFK"
     gene            complement(2223..3242)
                     /gene="recA"
                     /locus_tag="PLN75_07245"
     CDS             complement(2223..3242)
                     /gene="recA"
                     /locus_tag="PLN75_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRG03949.1"
                     /translation="MAKNKEENTSPVSEKLRALQLAMDKIDKDYGKGAIMRMGDSEVI
                     KVAVIPTGSIGLNAALGVGGYPRGRVIEIYGPEASGKTTLAIHAIAEAQKTGGIAAFI
                     DAEHAFDRYYAEKLGVNTDELLISQPDDGEQALEIADQLIRSAAVDIVVIDSVAALTP
                     RKEIEGDMGDSNVGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPETT
                     TGGNALKFYSSVRLDIRRVSQLKDGDDAIGNQVRVKVVKNKVAPPFRKAEFDIMFGEG
                     ISRAGEIVDLGVEYGIVKKSGSWFSYGDTKLAQGRDATKNVMKENPDLAAELEAKIME
                     AITTK"
     gene            complement(3317..5632)
                     /locus_tag="PLN75_07250"
     CDS             complement(3317..5632)
                     /locus_tag="PLN75_07250"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRG03950.1"
                     /translation="MDFLNELNKSQLEAVTYTDGPSLVIAGAGSGKTRVLTYKIAYLL
                     TQGVSPASILALTFTNKAAREMRRRIGELVGFQTSRYLWMGTFHAIFSRILRNEASLI
                     GYTKDFTIYDTSDSKSLIKSIIKEMKLDDKIYKPGNIHSRISSAKNNLYTAQDYVKNP
                     DFLKSDIQSRIPRTGEIYAIYANRCKQANAMDFDDLLLQTNILFRDHPQILAKYQHSF
                     AYILVDEYQDTNFAQYLIVKKLAERHERICVVGDDAQSIYSFRGANIDNILKFQQTYR
                     NARLFKLEQNYRSTKNIVKAANSLIDKNSEQIRKNIFSEKEEGNLIQVTSVLTDFEEA
                     FNVANSIARLQKTENLTYSHIAVLYRTNSQSRVMEEALRKQNIPYRIYGGLSFYQRKE
                     IKDVISYFRLVTNSNDEEALKRVINYPARGIGDVTVDKLSSTAQLHGVSIWSVLHDML
                     KYNLNVNAGTATRLAQFRDMIDVFAAQIDTLDAYELADSIVKTTGVIADAMNDRSAES
                     LSRVDNVQELLKAIHEFSENKRKEEHAETVTLTDFLSEVSLLTDQDTEKDENEDKVTL
                     MTIHAAKGLEFRCVFIVGMEEQLFPSPYAETPREMEEERRLFYVAITRAEENCFISYS
                     KSRFRNGQTNFANPSRFLKDIDRKFLEYNDEVSTTTDKNAFFNRWDDEVEIERNRFRQ
                     NSFRPASSEPLKEPAQPRKLKKMSPSTESETPVENTNLPVGTFVKHGIFGIGKVIKTT
                     LADGNEKAEIDFGEKGVKSLLLKFARLEILK"
     gene            5904..>6377
                     /locus_tag="PLN75_07255"
     CDS             5904..>6377
                     /locus_tag="PLN75_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRG03951.1"
                     /translation="MNLSRWILKLAGWKSVYQVEEPAKSVICVAPHTSNWDFIIGKLY
                     YWSLNKKAGFLMKKSWFFFPMGYLFRAMGGVPIDRSKRTSVTQQMVEEFNRRSVFHLA
                     ITPEGTRKPRAEWKKGFYYIAKGARVPIQLAYLDFGKKEMGITEVFYPTDDEEADF"
BASE COUNT         1508 a         1380 c         1456 g         2033 t
ORIGIN      
        1 ttttaaagta tttcaggttg atttgagcgt cctgaatggt ttcagcattg aatttataag
       61 atgctttcgg gttattagaa ctgatttgaa tttcggcagt gcccgcatta ttcataaaac
      121 gggctccgat aaagttcatc atcgaagtcg gccggttggc cgaaaaacct ttcataatgc
      181 tgatgtcggt gttgaaatcg cgtccctgcc cccatttgtt tttatgataa actatttctg
      241 ttcggatttt cgcagccaga ctgtcgagaa tggcatttgc ccggttcact tcgaaagttg
      301 atgaaatctg gattgcaaat ttgtcaataa atcgcttcct gaatgtttca ttgagcacaa
      361 gtcgccgcag cagcagtgtt gaccagggcg catttgccgg caatgatcct gcttcttcgc
      421 ccagcgccca gattaatgtg ttgtgtgtgt gatcattacc ccagatgttg taaccgaaat
      481 cggtatcgta aagtatccag cgccatttgc caccgtcttt tttcttccag gcttttacgt
      541 tgttgtgagg ccagtcagta tttcgcagat atatttctgt aaggaaataa tccatgaaat
      601 tatccatatc catcatagta caaacattgt cgtaaacagc tttttgggta atatcgctgt
      661 tggatacgta gtttgtcagt tttttgaatt cggaatcgta gtccatttcc cacgattcaa
      721 ccaggaagat atcttcttcg tcgtatccgt agtttgaata cacaaaatct tcgtcgctgc
      781 gttcgcgaag gttctgaatg ccgtagtaaa cgccattcat aaagcataca gccggttcat
      841 aggcaataca gtcgagattc atgcgcttca tcacgagcga ttgcatgtat ccgtcgcgca
      901 tcatggagtg atcgaagtcg ttgcctgaat tgcgcagcat gatgctccgg tatttcattc
      961 cgggtttggt ggctttgaaa atatcgtaat caaatttatt atctccgtat ttcttggttg
     1021 gatttagtat cagtgatttc tgtccgttca tgcgtgtcca tccgccggct atccggatat
     1081 ccacttcctg attgatgcgt gaaacgttgg tggtgtcata cagttcgatg tttgctgccc
     1141 ggtcccagtc ctgattccag ttggcgggtg tattcatacc gttaccggta atgccgtttg
     1201 ttccttgcac ataaatgcct atcgtgttgt cggtcaggtt tttctgttcg gtgaccactg
     1261 aaacgaccgg cagtctgaaa tttcgttcgc cgataaaata ggtgcaactg gctatgtcgc
     1321 ttgatagctt gttgcgcgaa aagcatcgtg ctctgattat tgaagttcgg gagatgctga
     1381 tgacagatcc gggtgtgtaa cgaatgtgac ttctggtggg ttcagcacca tttgtggtgt
     1441 aataaatggt atcgcctgtc atcggcgaat ccagtgtaac gtatataggt gtgttgtaga
     1501 atccgccttt ggtcacgaat accggtttgg cacaacgctc cgttgcgctt tttttcccgt
     1561 tgttggttaa tccggagctg tattgttcaa aataggtcca ttcgaggctg ccatcgctta
     1621 cccttccgta tgaaatgttg cggtactgtg cgggatacat cacgttatca atcacttgtc
     1681 cgctctcatt gagcaggtaa agtacaccgc cttcgggctt tagcctgaaa ctggcgtgtc
     1741 cgcttctgtc gctcctttcg aaccacagta cgctgtatcc gcggggtgaa atcattttcg
     1801 ataccggagg atgccatttt ttaggttgag tgagatcgtc ggtgaagtaa tagctatact
     1861 gattataaga cgtggtggtg cttgtattat aaagttccac ccacattgaa taattgtaac
     1921 tgtcatctat cactgcagaa acattgtttg tcatcaactc gtttattttc agttgagcaa
     1981 caagggtgtt ggttagtaag actaataaca gaaaaagtac ctgttttttc atggttaatg
     2041 gctatgagat ccttcattcg gactacaata tctgttccga acttattgaa atgatgttat
     2101 tttaaaaaag gaataaggga aaccgagatt cccttattcc tttgatgtaa tgacagttat
     2161 aattttttac aaaaaaagat cagaatcaga tgtgattaat ctgtatgtat ttaaggtgtt
     2221 ttttattttg ttgtgatggc ttccatgatt ttggcttcca gttcggcagc cagatccgga
     2281 ttttctttca tcacattttt ggtggcatca cgtccctgcg caagttttgt atcgccatag
     2341 ctaaaccacg agccgctttt tttcacaatg ccgtattcca ctccgagatc aactatttca
     2401 cctgcccgtg agattccctc accgaacata atgtcaaatt cagctttgcg gaatggcggt
     2461 gctactttgt ttttcaccac ttttactctc acctgattac cgatagcatc gtcgccgtct
     2521 ttcagctggc ttacgcggcg aatatccaga cgaaccgaag aatagaattt gagggcatta
     2581 ccaccggtag ttgtttccgg atttccaaac attacgccta ttttctcacg caactgattg
     2641 ataaagatac atgtagtatt tgttttgctg atgttggcgg taagttttcg cagcgcctgc
     2701 gacatgaggc gggcctgaag tccgacgttg gaatcgccca tatcaccttc aatttctttg
     2761 cggggcgtta gtgcagccac cgagtcaatc acaacaatat ccacagctgc cgaacggatg
     2821 agctgatcgg ctatttcgag tgcctgttcg ccgtcgtcgg gctgagaaat gagcagttca
     2881 tcggtgttca cacccagttt ttcggcatag tagcggtcaa atgcatgttc ggcatcaatg
     2941 aaagctgcaa taccacctgt cttttgtgct tctgctatgg cgtggatagc cagtgtggtt
     3001 ttaccggatg cttcaggtcc gtaaatctct atcacacgtc cgcgtggata gcctcccacg
     3061 cctaatgctg cattcagacc aatggatccg gtaggtatta ccgccacttt gatcacttcg
     3121 ctgtcgccca tgcgcataat ggcacctttg ccgtaatctt tatcaatttt gtccatagcc
     3181 agctgtaaag ctctcagttt ctcgcttacc ggactggtgt tttcttcctt gttcttagcc
     3241 atattgtttt ttttcaaaaa ttgtttttag atatttttct tacaaagata aaaattttta
     3301 tcagaacttt ttacatctac ttcaatattt ccagcctggc aaatttcagc agcaatgatt
     3361 ttaccccttt ctcgccgaaa tctatttccg ctttttcgtt accatcggca agggtggttt
     3421 taatcacttt tccgattccg aaaattccgt gttttacaaa tgttcctaca ggcagatttg
     3481 tgttttctac cggcgtttca ctttcggtgg aaggactcat ttttttaagt tttcgtggct
     3541 gtgccggctc ttttaacggt tcggatgaag cgggtctgaa ggaattctgc ctgaaccggt
     3601 tacgttcaat ttccacctca tcgtcccacc ggttgaagaa cgcatttttg tcggtcgtgg
     3661 tgcttacttc gtcgttgtat tccagaaatt ttcggtcaat gtctttcaaa aaacggcttg
     3721 gattggcaaa atttgtctgt ccgtttctga accgtgattt tgaataactg ataaagcagt
     3781 tttcttcggc ccgggtgatg gccacgtaga acaatcgccg ttcttcttcc atttctcgtg
     3841 gagtttcggc ataaggcgac gggaaaagct gttcttccat gcctacaatg aatacgcatc
     3901 tgaactccag tcctttagct gcatgaatgg tcatcagtgt cactttatct tcgttttcat
     3961 ctttttcggt gtcctggtcg gtgagcagcg agacttccga aaggaaatcc gtcagcgtta
     4021 ccgtttcggc atgttcctct tttcttttgt tttccgaaaa ttcatgaatg gctttgagca
     4081 gttcctgcac gttgtcaacg cggctcaggc tttcggccga acggtcgttc atggcatcgg
     4141 ctattactcc tgtagtcttt acaatgctgt cggccagttc gtaggcgtcg agcgtgtcaa
     4201 tctgtgcagc gaacacgtca atcatgtccc tgaactgtgc cagtctggta gctgtgccgg
     4261 catttacgtt cagattgtat ttgagcatat cgtgcagtac gctccagata cttacaccgt
     4321 gaagctgcgc cgtactgctc agtttatcca ccgttacatc gccaatgccg cgtgccggat
     4381 agttgatgac ccgcttcagt gcttcttcgt cgttgctgtt tgttacgagc cggaaatagg
     4441 atattacatc ttttatttct tttcgctggt agaatgaaag tccgccgtaa atgcggtaag
     4501 gaatgttttg cttccgcagc gcttcttcca tcacgcgcga ttgcgaattg gtacggtaaa
     4561 gcaccgcaat gtggctgtat gtcagattct cagttttctg aagcctggct atgctgttgg
     4621 caacattaaa tgcctcttcg aagtcggtca gcacggaggt gacctgtatc agatttcctt
     4681 cttctttttc tgagaaaata tttttacgga tctgttccga attcttgtct atcaggctgt
     4741 tggccgcttt cacaatgttt tttgtcgatc ggtaattttg ttccagtttg aaaagcctgg
     4801 cattacggta tgtctgctga aatttcagaa tattgtcgat gttggctccg cggaatgaat
     4861 agatgctttg tgcatcgtcg cctaccacgc aaatgcgctc gtggcgttcg gcaagttttt
     4921 ttactatcag gtactgtgca aaattggtgt cctgatattc gtcaacgaga atataagcga
     4981 agctgtgctg atattttgcc agtatttgcg gatgatcgcg aaaaagaatg ttggtctgaa
     5041 gcagcaaatc gtcaaaatcc atggcatttg cctgtttaca gcggttggca tagatggcgt
     5101 aaatctcgcc ggtgcgtggt atgcgcgact ggatatcgga tttcagaaaa tcgggatttt
     5161 tcacataatc ctgcgccgtg tacaaattgt ttttagcact tgaaatacgc gaatggatgt
     5221 ttccgggttt atagatttta tcatccagct tcatttcttt gatgatgctt tttatcaggc
     5281 ttttgctgtc ggaggtgtcg tagatggtga aatctttggt ataaccgatc agcgatgcct
     5341 cgttgcgcag gatacgcgaa aaaatggcat gaaaagtacc catccacagg tagcgtgaag
     5401 tctgaaaacc aacgagttcg cctatgcgcc ggcgcatttc gcgggcagcc ttgttggtga
     5461 aggtgagtgc cagaatagaa gcgggtgaaa cgccctgagt aagcaggtag gctatcttgt
     5521 atgtaagtac gcgcgttttg cccgaaccgg ctccggctat caccagcgaa ggtccgtcgg
     5581 tgtaggtcac tgcttccagc tgacttttat ttagttcgtt gagaaaatcc atttgtagcc
     5641 ccctaaatcc ccctcaaagg ggactttttg gtttgtaaat taatatatca ggaagaaatt
     5701 aacgtgatat ttttgtttat ttttaaaaaa atagcaacaa cttttgctgc aatcaaagaa
     5761 atcaattaaa ccataaaaaa tcacagttca gacttcagtt cacaaaatta ctaatttgca
     5821 cttaattatc agaaaatagt tatcaacaac ttgctttttg aatgaaaata ggtagatttg
     5881 tgaaattttt aacagctaac gatatgaatt taagtaggtg gattttaaaa cttgccggtt
     5941 ggaaatcggt ttaccaggtt gaagagccgg ccaaaagtgt gatttgtgtg gctcctcaca
     6001 caagcaactg ggattttatc atcggaaaat tgtactattg gtcgctgaat aaaaaagccg
     6061 gttttctgat gaagaaatca tggtttttct ttcccatggg gtatcttttc agagccatgg
     6121 gcggggtacc gattgaccgg agcaaacgta cgtcggtaac gcagcaaatg gttgaggaat
     6181 ttaaccggcg gtcggttttt cacctggcca ttacccccga aggcacccgc aaaccacgtg
     6241 cagagtggaa aaaaggtttt tactacatcg ccaaaggcgc ccgagtgccc attcagctgg
     6301 cttatctgga ttttggcaaa aaggaaatgg gtattaccga agtcttttat ccaacggacg
     6361 atgaagaggc tgatttt
//
LOCUS       DAORKP010000121         6354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2365_length_6354_cov_8.861565, whole genome shotgun sequence.
ACCESSION   DAORKP010000121 DAORKP010000000
VERSION     DAORKP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_6354_cov_8.861565"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..480
                     /locus_tag="PLN75_07260"
     CDS             313..480
                     /locus_tag="PLN75_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRG03952.1"
                     /translation="MAYVINEDCIACGTCISECPVEAISEGDIYVIDPDICTDCGTCA
                     DVCPSEAIHPA"
     gene            complement(542..1612)
                     /gene="wecB"
                     /locus_tag="PLN75_07265"
     CDS             complement(542..1612)
                     /gene="wecB"
                     /locus_tag="PLN75_07265"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRG03953.1"
                     /translation="MKKIITIVGARPQFVKAATLSRQFSLLGVDEKIIHTGQHYDANM
                     SDVFFEEMEIPKPTYNLDIHGVGHGAMTGRMLEGIEEILLNELPDGVLVYGDTNSTLA
                     GALAASKLHIPVVHVEAGLRSFNMQMPEEINRILTDRISDLLLCPTDTAIHNLQREGF
                     NNFNTKIVKNGDVMQDAAIFYAAKAEEKSNVLKSINLSRYILATIHRQENTDSPENLQ
                     NIIVGLNEINRQTPVVVPIHPRTRNILAQLKIVPAFKLIDPVGYFDMIVLLKNCEMVI
                     TDSGGVQKEAFFFGKHCITMREQTEWVELVENGFNTLTGSDAEKIVTAYEQTKQKKSD
                     FSINLYGKGQAAEVAAKEIFSF"
     gene            complement(1667..2971)
                     /locus_tag="PLN75_07270"
     CDS             complement(1667..2971)
                     /locus_tag="PLN75_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG03954.1"
                     /translation="MKKKVLIVTYYWPPSGGAGVQRWLKFVKYLRDFGWEPIVYTPEN
                     PEYPSEDYSLYKDIPEGVEVIKTPIWEPYNIYRNLFGKKGQKINAGFISETKKSGWKE
                     RLSIWIRGNFLIPDPRLFWVKPSIKYLKEYLEHNPVEAIVTTGPPHSMHMIGLGLKKL
                     HPDVPWIADFRDPWTNIDFYKELNLTYLADKRHHQLEKQVIQSADCVVVVSNDMVDEF
                     SRLPHQRIEMITNGFDAADVSGRALTPDSMFTLSHIGTLNAARNPKTLWKVLGEICSA
                     DEKFRSDLKIQLIGKTDFSVLEDIEKLGLTPRLEKTEYLPHSEANARQQTSQILLLLI
                     NNTANAKGILTGKFFEYLSAGRPILAVGPVDGEVAKVLEDTQAGMIVDFEDEENTRKA
                     VLDYYNSYKQGNLKVAAKSIDKFSRKSLTGELAEIINTLATK"
     gene            complement(3024..4550)
                     /gene="gltX"
                     /locus_tag="PLN75_07275"
     CDS             complement(3024..4550)
                     /gene="gltX"
                     /locus_tag="PLN75_07275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRG03955.1"
                     /translation="MNRKVRVRFAPSPTGPLHIGGVRTALYNYLFARQHGGDMLLRIE
                     DTDSARFVPGAEEYIVEALRWLGIEIDEGIGAKNTGKHAPYRQSERKDIYKKYVNQLL
                     EAGLAYIAFDTPAELDMKRGKTANFQYDATTRNGMLNSLTLSDAEVKKRLEAGENYVV
                     RIKIEPNLEICVNDLIRGEVVINSSVLDDKVLYKSVDELPTYHLANVVDDYLMEVTHV
                     IRGEEWLPSAPLHVLLYRYLGWESVMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFP
                     LDWEDPNTEVISSGYREAGYFPEAMVNFLALLGWNPGNEQEIFSMQELINLFSLDRVS
                     KSGAKFDYEKGKWFNHKYLQMKSNEDLADIFQHVLVEKDIIEDSEKVIKVVALIKERA
                     DFVTDLWEQGFFFFQAPTSFDEKTVSKRWKENTPAQMNELIEKLQQIDDFSAANQEKI
                     VIQWIEEKGYGMGAVMNAFRLALVGAAKGPHLFDITEIIGKQETIARIKRAVSVLAQE
                     "
     gene            complement(4818..5822)
                     /gene="tsf"
                     /locus_tag="PLN75_07280"
     CDS             complement(4818..5822)
                     /gene="tsf"
                     /locus_tag="PLN75_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRG03956.1"
                     /translation="MAVTMAEISKLRTMTGAGMMDCKNALIEAENDFDKAIEIIRKKG
                     QAVAAKRSDREASEGCVLAGEKDGFAAVLALKCETDFVAKNADFIALTQSILDKAIAE
                     KPESLDALKALTFTADGRTIADLVTERSGITGEKLELDYYEFVKGGTVTTYIHPGNKL
                     ATIVAFAENDAEYETIHGVAMHIAAMSPVAIDESAVPQKIKDNELAVAIEKTKTELVA
                     KEVEVALKKAGINPAHVDSDDHIESNMAKGWITAEDAAKAREIKSTVVAEKSASLPEQ
                     KVNSIAAGRMAKFFKEYTLLEQKYEGGGEEAGKTTVKEVLAKKHLTCVAFKRVTLNQE
                     "
     gene            complement(5990..>6354)
                     /gene="rpsB"
                     /locus_tag="PLN75_07285"
     CDS             complement(5990..>6354)
                     /gene="rpsB"
                     /locus_tag="PLN75_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRG03957.1"
                     /translation="PSALFVVDVMKEQIAVKEAQRLGIPVFGIVDTNSNPNEVDFVIP
                     ANDDATKSVEVILNAICAAVQEGLEERKIEKTDSEAAEAPEAKERKSRISKRPRTQKE
                     DDDAMKANVVKKFVKEEE"
BASE COUNT         1616 a         1418 c         1264 g         2056 t
ORIGIN      
        1 gataaaaaaa acattttcta ataaaaaata tcattgtcag gtaaacattt tagaaaattc
       61 ctgcaagttt ttctaatatg tgacttacca taatgtcgcc tctacgaggc tttaattttt
      121 agcaccagcg gtgcgacaat ctggtagaaa acagattaca cccatcaata aaagctccat
      181 cggagcgaca ttattttttc tttttctttt atcagacaat aaaattaaaa aaaatctcaa
      241 aatctttttt taggaataga atttgttata catttgcaca actaattagt actaacaaaa
      301 atttttcaga aaatggctta cgtaattaat gaagattgta ttgcttgcgg tacctgcatc
      361 agcgaatgtc cggtagaagc aatttcagaa ggtgatatct atgttattga tcctgatatc
      421 tgtacagact gcggtacttg tgctgatgta tgtccctcag aagcaatcca tccggcgtaa
      481 attaaatacg caaaaaacta aaaaaatcct gtaagtgtat cacttacagg atttttgttt
      541 ttcaaaagga aaatatttcc tttgctgcca cttcggctgc ctgtcctttt ccgtacaggt
      601 taatcgaaaa atcggatttt ttctgtttgg tttgctcata agcggtcact attttttcgg
      661 catcacttcc ggtcagggtg ttaaacccat tttcgacaag ctccacccat tcggtttgtt
      721 cgcgcatggt tatgcagtgt tttccaaaga aaaaggcttc tttctgtacc ccgccactat
      781 cggtaatcac catttcgcag tttttgagca gcacaatcat atcaaaataa cccaccggat
      841 cgatgagttt aaacgctgga acaattttca gttgcgctaa aatattccgg gttcgggggt
      901 ggataggcac tacaacaggt gtttgtcggt ttatttcatt caaaccgaca attatatttt
      961 gtaaattttc aggtgaatca gtattttcct gacgatgaat agttgccaaa atataccgcg
     1021 acaagtttat tgatttcaat acgtttgatt tctcttctgc ttttgcagcg tagaaaatag
     1081 cggcatcctg cattacatca ccattcttta caattttcgt attaaaatta ttaaaacctt
     1141 ctctctggag gttatggatg gcagtatcag tcggacaaag cagcaaatca gaaatcctgt
     1201 cggtcaggat gcggttaatt tcttcgggca tctgcatgtt gaaggagcgc aatccggctt
     1261 ccacatgcac caccggtata tgaagcttgg atgctgccag tgctccggca agtgtagaat
     1321 tggtatcgcc gtacacaagc actccgtcgg gtagttcgtt cagcagtatt tcttctattc
     1381 cttccagcat ccgaccggtc atagcgccgt gtccaactcc gtgtatatca aggttgtagg
     1441 ttggttttgg gatttccatc tcttcaaaaa aaacgtccga catattggca tcgtaatgct
     1501 gaccggtgtg aattattttt tcatccaccc caagcaggct gaactgacga ctcaatgtgg
     1561 ctgctttcac aaactgtggt cgtgcaccca cgatggtgat tatttttttc atacgattaa
     1621 ttgcccccta accccctaaa gggggaataa gaagggagat aatttcttat ttagttgcta
     1681 atgtattaat tatttcggct aattctcctg taagcgattt acgagagaat ttatcgattg
     1741 atttggcagc tactttgaga tttccctgtt tgtaactgtt ataataatcc aaaacggctt
     1801 ttcgggtatt ttcttcgtcc tcaaaatcaa caatcattcc ggcctgagta tcttcaagca
     1861 cctttgccac ttcgccatcc acaggaccga cagccaatat gggtcttccc gccgagagat
     1921 attcgaagaa tttcccggtc agaattcctt ttgcatttgc cgtattgtta atcagcaaaa
     1981 gcaaaatctg cgatgtctgt tgcctggcat ttgcttcgct gtgcggcagg tattctgttt
     2041 tttccaaccg aggagttagg cctaactttt caatgtcttc gagaacagaa aagtccgttt
     2101 taccaatcag ctgaattttc agatcggagc ggaatttttc gtctgccgaa caaatttctc
     2161 cgagtacctt ccagagcgtt ttcggatttc tggcagcatt gagcgtaccg atgtgcgaaa
     2221 gggtaaacat gctgtcagga gtgagcgctc tgccgcttac atccgcggca tcaaatccgt
     2281 tggtaatcat ctctattctt tgatgaggaa ggcgggaaaa ttcatccacc atgtcgttgg
     2341 aaaccactac cacacaatca gctgattgaa tcacctgttt ttcaagctga tgatgtcttt
     2401 tatcggctaa gtaagtcaga ttaagctcct tgtagaagtc gatgttggtc cacggatctc
     2461 taaaatcagc gatccacgga acatccgggt gcagtttttt taatccaaga ccaatcatgt
     2521 gcatggaatg cggaggaccg gtagttacga ttgcttccac cggattatgc tcaaggtatt
     2581 ctttcaggta ttttatggat ggttttaccc aaaaaagtcg cggatcggga atcagaaaat
     2641 ttcctcttat ccagatggac aaccgttctt tccatcccga tttttttgtc tcggagataa
     2701 agccggcgtt gattttctgg cctttttttc cgaacaggtt tcggtaaata ttataaggtt
     2761 cccaaatcgg tgttttaatc acttcaacgc cttcagggat gtctttgtaa agcgagtaat
     2821 cttccgaagg gtattccgga ttttcagggg tgtatacgat gggttcccat ccgaaatcac
     2881 gcaggtattt tacgaatttc aaccaccttt gcacgcctgc cccacctgag ggtggccagt
     2941 aataagttac aatcaatacc ttttttttca tctgaatgtg cattatgtta gcgcaggaaa
     3001 tcagccggat tgctaactta cagctattcc tgagctaaaa cactcacagc ccgttttatt
     3061 cttgcaattg tttcctgctt cccgataatt tcggtgatat caaataaatg cggacctttg
     3121 gcggctccca cgagtgccag acgaaaagcg ttcattacag ctcccatgcc atagcctttt
     3181 tcttcaatcc actgaatgac aatcttttcc tgattggcgg cagaaaaatc gtcgatttgc
     3241 tgcaattttt caatcagttc gttcatttgt gcaggcgtgt tttctttcca gcgtttgctt
     3301 acggtttttt cgtcgaagga agtcggtgcc tggaagaaga aaaagccctg ctcccagagg
     3361 tcggtcacga aatcagcccg ttcttttatc aatgctacca cttttatcac tttttccgag
     3421 tcttcgataa tgtctttttc taccagcaca tgttggaaaa tatcggcaag atcttcgttc
     3481 gacttcattt gtaagtattt atgattaaac cacttacctt tttcataatc aaatttagca
     3541 ccgcttttgc ttacgcggtc gagcgagaaa agattaatca gttcctgcat cgaaaatatt
     3601 tcctgctcgt ttccgggatt ccatccgagc aatgccagga aatttaccat tgcttcgggg
     3661 aaatagcctg cttcacgata acctgaagaa atgacttcgg tgttcggatc ttcccagtcg
     3721 agcgggaaaa cagggaaacc aagccggtca ccatcacgtt tgctcaactt accgttacca
     3781 tcgggtttga gcagcagcgg caggtgggca aactgcggca taacactctc ccaacccagg
     3841 tagcggtaga gcaacacgtg aagcggtgcg gatggcaacc actcttcgcc gcggataacg
     3901 tgtgtaacct ccatcagata atcatccacc acgtttgcca ggtgataggt aggcaattca
     3961 tccactgatt tgtaaagtac cttatcgtcc agtaccgagg aattgattac cacttctccc
     4021 cgaatcaaat cattgacaca aatttcgaga tttggttcaa tttttatacg taccacataa
     4081 ttttcaccgg cttcaagtct tttcttaact tctgcatcgg ataatgtcag tgaattcagc
     4141 attccgttgc gtgtagtggc atcgtattga aaattagctg tttttccacg tttcatatcg
     4201 agttcggcag gagtatcgaa ggcaatgtaa gccaaacctg cttccaggag ctgattaacg
     4261 tattttttgt aaatatcttt tcgctcactt tgccggtagg gagcatgttt tccggtattt
     4321 ttagcaccta ttccttcgtc aatttctatt ccaagccacc ggagtgcttc tacgatatac
     4381 tcttcggctc cgggcacaaa acgggccgaa tcggtatctt caattcgcaa caacatgtcg
     4441 ccgccatgct ggcgtgcaaa caaataatta tacaaagccg tacgaactcc gcctatatgc
     4501 agcgggccgg ttggactcgg ggcaaatcgc accctcactt ttctgttcat tataaaatat
     4561 ggatgattta actgcaaaat tacgtaaaag tttagtaagt tcaaaagttt gaaccttcta
     4621 ttgataaaag acccacctgc ttaccaataa ctgacagcaa ttgtttcctg ttgaatgaca
     4681 tcaaaaaaaa acctttcgga ttcatccgaa aggtttttgt tcttgttctc ctaaaacaat
     4741 cattccattt tctttctcct tttggagaaa gtatccgtcg aatgacggag gatagaggtg
     4801 tggggtcagg agtgatttta ttcctgattc agggttacac gtttgaaagc cacgcaggtt
     4861 aaatgttttt ttgcaagcac ttcttttaca gttgtttttc cggcttcttc gccgccacct
     4921 tcgtattttt gttctaaaag ggtatattcc ttgaagaatt ttgccatacg tcctgcagca
     4981 atgctgttca ctttctgttc gggaagagac gctgattttt cagcaacgac tgttgacttt
     5041 atttcgcgtg ctttggctgc atcttctgca gtaatccagc cttttgccat gtttgattct
     5101 atgtggtcat cgctatcaac gtgggcagga tttatgccgg cttttttcaa cgcaacttca
     5161 acttcctttg caaccagttc ggttttggtt ttttcgatag ctactgccag ctcgttatct
     5221 tttatcttct gaggtacagc agattcatca atagctacag gtgacatagc cgcaatgtgc
     5281 attgccacac cgtgaatggt ttcgtattct gcgtcatttt cagcaaatgc aacgattgtg
     5341 gccagtttgt tgcccggatg aatgtaagtt gttactgtgc cgccttttac aaattcataa
     5401 tagtccaact ccagtttttc gccggtaatt ccggaacgtt cagttacaag gtctgctatt
     5461 gtacgcccgt cagcagtaaa agtcaacgct ttaagtgcat ctaaactttc aggtttttct
     5521 gctattgctt tatcgagaat tgattgtgtc agtgctataa aatcagcatt ttttgccaca
     5581 aagtcagttt cacatttcag tgctaataca gctgcaaatc catctttttc ccctgcaagc
     5641 acacaacctt cagatgcttc acggtcgctg cgttttgctg ctacagcttg tcctttttta
     5701 cgaataattt ctattgcttt atcaaaatcg ttttcggctt cgatgagtgc gttcttgcaa
     5761 tccatcattc cggcacctgt catggtacgc aattttgaaa tttctgccat tgttactgcc
     5821 ataataataa gtttttagat accgacccct aaaccctgat tggagataaa agtcttgtat
     5881 gtttgttaat tttttttcta tttaaaaaag gttacaagct acaactaaat tataacccgt
     5941 aaccctgttt ttagtacccg ttttcccctt caggggttgg ggctgttttt tattcttctt
     6001 cttttacgaa ttttttcacc acattggctt tcatcgcatc gtcgtcttct ttttgagttc
     6061 ttggtctctt cgaaatgcgt gatttccttt cttttgcttc cggtgcttcg gcagcttcgg
     6121 agtcagtttt ctcaatctta cgttcttcaa gtccttcctg aacggctgca caaatggcat
     6181 tgagaattac ttcaactgat tttgttgcat cgtcgttggc cgggattacg aagtccactt
     6241 cgttcggatt tgagttggta tcaacgattc cgaaaacggg aataccgaga cgttgagctt
     6301 ctttcactgc gatttgttct ttcatcacat ccaccacaaa gagtgctgaa ggca
//
LOCUS       DAORKP010000122         6348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2369_length_6348_cov_8.741300, whole genome shotgun sequence.
ACCESSION   DAORKP010000122 DAORKP010000000
VERSION     DAORKP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_6348_cov_8.741300"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1179
                     /locus_tag="PLN75_07290"
     CDS             301..1179
                     /locus_tag="PLN75_07290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRG03958.1"
                     /translation="MKKQLKHIFAEFMEKMRFKYRISIVNENTLEETWYTRLSRISVV
                     LFVTGFFLLTFIFLTLIIFATPLRHYLPGFEDSGNRAVIVQQSIRVDSLENQLQLMGS
                     YYQVLKNHISETTSVDSVKSLDSVTIRERATVLMEKSKRESEFVKQYEETEKYNLGSI
                     NTIESDKKVFVFFRPVEGVVASTFNPKEEKFGISIITSPQETVKSVLEGRVVYAGYTF
                     DNQWVIHLQHDNDYLSIYKNNTRLLKNVGDYVKAGQSIAITGSDENVGTNKHFYFELW
                     KKGTPVNPQDVITFRF"
     gene            1247..2413
                     /locus_tag="PLN75_07295"
     CDS             1247..2413
                     /locus_tag="PLN75_07295"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRG03959.1"
                     /translation="MADRKKNIAILGSTGSIGTQALDVVRSNPERFEVFALTANNNLE
                     MLIKQAVEFQPEVVAIANESHYLQLRQAVADLPVKVFAGKEAIAQLVEMEPIDLVLTA
                     MVGYAGLKPTINAIKAGKTIALANKETLVVAGEIINELALKHKTPILPVDSEHSAIFQ
                     CLAGEGDNSVEKIILTASGGPFRTKTLEELRYVTSAQALKHPNWDMGAKITIDSASMM
                     NKGFEVIEAKWLFGLKPDQIDVVVHPQSIIHSMVQFADSSIKAQLGMPDMKLPIQYAF
                     TYPERLPTEFPRVDFMKYPMLTFEKPDQQRFRNLALAYRAMENGGNMPCILNAANEIV
                     VAAFLKNRIGFLQMSDIIENVMQKTTFIKKPTYDDYVLTDDEARQITEKEINQM"
     gene            2497..3966
                     /gene="rseP"
                     /locus_tag="PLN75_07300"
     CDS             2497..3966
                     /gene="rseP"
                     /locus_tag="PLN75_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRG03960.1"
                     /translation="METFLIRAAQLILSLSILVVLHELGHFAFARIFKVRVEKFYVFF
                     NPKISLIRAKKILGKWNIRFFAQNVLPNERIKLDSNGEPLMNGKNHVMEPVPESELPE
                     GDWRRYPETTEWGIGWLPLGGYCKIAGMIDESMDKTQLTQEPKPWEFRTQPVWKRLFI
                     ISGGVLVNFVLALFIYSVILFTWGREYIPLENATNGLSFSQTMIDAGFRDGDKITAID
                     GKKPLELSDAIEMILIDHATTVTVDRSGTPVTIEIPENLTQKALASEKGVMTYRMPFV
                     IDTVVTGSLASKAALMKGDSLVAVNGKLINMQQEVIREFSENKGKKISFDYYRQGTLY
                     RTTLTMGADGKLGVGWRNFLKTERVEYGFFEAIPAGIGIGVQKLVGYVKQFKLVFTKE
                     GVKSIGSFGALGQLFPATWDWLSFWELTALISIILAFMNILPIPGLDGGYIVFLIYEM
                     ITGRKPSDKFMEVAQMIGMTLLMLLFVYAIGNDIFRAFK"
     gene            3977..4333
                     /locus_tag="PLN75_07305"
     CDS             3977..4333
                     /locus_tag="PLN75_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HRG03961.1"
                     /translation="MITILCYPTCGTCRKAENWLKENGIKYNYRHIKEENPGFEELQL
                     WKEKSGLPVSKWFNTSGLLYKEHNMKEKVKSLAEDEQLRMLASDGMFVKRPILLSGET
                     VLVGFKQEEWEKLLKK"
     gene            4499..4933
                     /locus_tag="PLN75_07310"
     CDS             4499..4933
                     /locus_tag="PLN75_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRG03962.1"
                     /translation="MKKTLFSVFVLMFLAVSASAQVDKIVGKWKTIDDKDGSEKSIVN
                     IFKASNGKYYGKVEKLFKEPNKKCTECEGENKDKPVLGMTVLNNMVEKDGTLTGGTII
                     DPANGKVYKCNVSLDTNTGKLKVRGSLDKRGVIGRTQHWVKN"
     gene            complement(5015..>6348)
                     /locus_tag="PLN75_07315"
     CDS             complement(5015..>6348)
                     /locus_tag="PLN75_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRG03963.1"
                     /translation="WLPTLRTHAMPGGIVKRDVYEKLVNQTLEMLKKSLPLDGLFFDI
                     HGAMSVQDLEDPEGDFIVRIREVIGNEVLISTSMDLHGSVSPRLAENSDLITCYRLAP
                     HEDAMISKKRALTNLLDRLENGKGKPAFKAIIPVPILLPGEKTSTRIEPAKSLYELIP
                     TVIDGEKVIDAAIWMSYPWADEPRNHAVVMAYGDDKEAVGKAAEKLANHFWNVRDQFE
                     FVAPTAYLPECIEKALASTKKPFIISDMGDNPTAGGAGDVTWTLTELLKRPEFKSENG
                     KTVIYASIPDSGFVKKAVAIGVGGKIDATAGARVDNRYAPPVRIAGTITAIVNDEKDG
                     NTVVVVKTGSVSVIVTKKRKAYHYESYFTQAGLNPRETDILVVKIGYLVPELYDMRGD
                     WIMALTPGGVDQDLTRLPYKNIRRPMYPLDKDMAQPDLNAEFVPLAGKSKK"
BASE COUNT         1941 a         1333 c         1376 g         1698 t
ORIGIN      
        1 gaatacctgg atgttaaata acacaatgaa ggttcagatc agttttttta tcagataata
       61 atcaccattt tatactgcct ggtgttgttt ttttaagata gtagttgtat taatttaatc
      121 aaaggaacga aagattcaaa tcatgatatc ttatgaatga ataaacatgc aaacgggatg
      181 cttaggataa tcttctgaat tttaaataat taacgaaata aaggatactt atatatttat
      241 gttttgttca ttttcttaaa aatagctaac tttgcgggca tttttgtact gaacagatgc
      301 atgaaaaaac agctgaaaca catatttgct gagtttatgg aaaagatgcg gtttaagtat
      361 cgtatctcca ttgtgaacga aaatacgctc gaagaaacct ggtacacccg cttatcacgc
      421 attagtgttg tattgtttgt gaccggtttt tttctgctca catttatttt cctcactctc
      481 atcatttttg caactccgct gaggcattat ctgcccggat ttgaagactc cggtaatcgg
      541 gcagttatcg ttcaacaatc tattcgtgtt gattcgctgg agaatcagtt gcagttaatg
      601 ggctcgtact atcaggtact aaaaaatcac atttctgaaa ccacatcggt tgatagtgta
      661 aaatcactcg attcagttac catcagagag cgtgcaaccg tactgatgga aaagtccaaa
      721 agagaatcgg agtttgtaaa gcagtatgaa gaaaccgaaa aatacaacct cggatccatt
      781 aatacgatag aatctgataa aaaagtgttt gttttctttc ggcctgtaga gggagtggtg
      841 gcttcaacgt ttaatccgaa agaggagaaa ttcggaattt caattattac ctcacctcag
      901 gaaacagtaa aaagtgtact ggaaggaagg gtggtatatg ccggttatac atttgacaac
      961 caatgggtta ttcacctgca acacgacaac gattatttat ccatttataa aaacaacacc
     1021 cggttgctca aaaatgttgg cgattacgtt aaagcaggtc agagcattgc cattaccgga
     1081 agtgatgaaa acgtagggac caacaagcat ttttattttg aattgtggaa aaagggaact
     1141 cccgtgaatc cgcaggatgt tattacgttc aggttttaat cgtgaatgta ttaaatggaa
     1201 agtatcggat tatttattca gaaatagagc aaattaaata aaaaaattgg ctgacagaaa
     1261 gaaaaatatt gccattctgg gctccaccgg ttctatcgga acgcaggcgt tggatgtggt
     1321 gcgctctaat cccgaacggt ttgaggtgtt tgcgcttaca gccaacaaca atctcgaaat
     1381 gctcataaag caggccgttg aatttcagcc cgaagtggtt gccattgcca atgaaagtca
     1441 ttatctgcaa ctcaggcagg cagttgctga tttgcctgtc aaagtttttg ccggaaaaga
     1501 ggccattgct cagttggtag aaatggaacc aatcgatctg gtactcaccg ccatggtggg
     1561 atatgccggt ttgaaaccca ccatcaacgc cataaaagcc ggaaaaacca ttgcgctggc
     1621 caacaaggaa accctggtag tagccggaga aatcatcaac gagcttgcgc tgaaacacaa
     1681 aacaccgatt ctgcctgttg attccgaaca ttctgcgata ttccagtgtc tggcaggcga
     1741 aggcgacaat tccgttgaga aaatcatact tacagcatcg ggcggacctt tcaggacgaa
     1801 aaccctggaa gaactccggt acgtaacgtc ggcccaggct ctcaaacacc ccaactggga
     1861 tatgggtgca aaaattacaa ttgattcggc aagcatgatg aacaaaggct ttgaagtaat
     1921 tgaagccaaa tggcttttcg gtctgaaacc ggatcaaatc gatgtggtag tgcatccgca
     1981 atccatcatt cattccatgg tgcaatttgc cgacagctct atcaaggcac aactcggcat
     2041 gcccgatatg aagctcccca ttcagtatgc cttcacctat ccggaacgac tgccaactga
     2101 atttccgagg gtggatttca tgaagtatcc gatgcttact ttcgaaaaac ccgaccagca
     2161 aaggttccga aatcttgctc tggcctaccg cgcgatggaa aacggaggaa acatgccctg
     2221 tatcctgaat gccgccaacg aaatagtggt agctgcattt ctgaaaaaca gaatcggatt
     2281 tctgcaaatg agtgatatta ttgaaaatgt gatgcagaaa accacattca tcaaaaaacc
     2341 aacttacgac gactatgtgc tcaccgacga tgaagcccgg cagataacag aaaaggaaat
     2401 aaaccagatg taaaataata atcaaaaata ccttgattgt tagttgatta tattttttaa
     2461 aaaaaaagaa aacagtaaaa aaagaaaata aaatagatgg aaacattttt aatacgcgcg
     2521 gcgcaactca ttctgagttt atcaatttta gtagtgctgc atgaattggg tcactttgct
     2581 tttgccagaa tttttaaagt aagggtggag aaattttacg tgttcttcaa tccgaaaatc
     2641 tcactaatca gagccaaaaa gattctcggg aaatggaaca tccgcttttt tgcacagaat
     2701 gtactgccaa acgaaaggat taaactcgac tcaaatggcg aacccttaat gaatggtaaa
     2761 aaccatgtaa tggaacctgt acccgaaagc gaattgcccg aaggcgactg gcgccgctat
     2821 cccgaaacca ccgaatgggg catcggatgg cttccactcg gaggatactg caaaattgcc
     2881 ggaatgatag atgaatccat ggataaaacc cagcttactc aggaaccaaa accctgggaa
     2941 tttcgtacac aaccagtttg gaaacgactt tttatcattt caggcggcgt gttggtcaat
     3001 tttgtattgg cgctttttat ctattcggtt attctcttca cctgggggcg cgaatacatt
     3061 ccgcttgaaa atgccactaa cggactgagt ttctctcaaa caatgattga tgcgggtttt
     3121 cgggatggtg ataaaataac agccatcgac ggcaaaaaac cactcgaact ctcggatgcc
     3181 atcgaaatga tattgattga ccatgccacc acggttactg tggatcgcag cggaacgccc
     3241 gttacaattg aaatccccga aaatctgacc cagaaagcac tcgcatccga aaaaggggtg
     3301 atgacttacc ggatgccctt tgtcattgat accgtagtga ccggatcact cgcctccaaa
     3361 gcagccctga tgaaaggcga cagccttgta gcagtcaacg gaaagcttat caacatgcag
     3421 caggaggtaa tcagagaatt ttcagagaac aaaggaaaaa agatttcttt tgattactac
     3481 cggcagggaa ccctttacag aacgacactt acgatgggtg ccgacggcaa actgggagta
     3541 ggctggcgta attttttgaa aaccgaaagg gttgaatacg gatttttcga agccattccc
     3601 gccggcatag gcattggtgt gcaaaaactc gtgggctatg tgaaacaatt caaactcgta
     3661 ttcaccaaag aaggcgtgaa atccatcggc agcttcgggg cgctcggtca gctattccct
     3721 gccacatggg actggctcag cttctgggag cttacggcac tcatttccat catcctggcg
     3781 tttatgaaca ttctgccaat ccccgggctg gacggcggtt acatcgtttt tctcatttac
     3841 gaaatgatta caggacgcaa accgagcgat aagttcatgg aagtagcgca aatgattggt
     3901 atgacattgc tgatgctgct ctttgtttat gccatcggaa acgatatttt cagagccttc
     3961 aaataaaaat tcaacgatga ttacaatctt gtgttaccca acatgcggca cttgccgtaa
     4021 agccgaaaac tggctgaagg aaaatggtat aaaatacaat taccggcaca ttaaagagga
     4081 aaatccgggt ttcgaagaac ttcaactctg gaaagaaaaa agcggactcc ctgtgagtaa
     4141 gtggtttaat accagcgggt tgctctacaa agaacacaat atgaaggaaa aggtaaaatc
     4201 acttgccgaa gatgaacagc ttcggatgct cgcttcggat ggcatgttcg tcaaacgccc
     4261 cattctgctt tcgggtgaaa ctgtattggt aggatttaag caggaagagt gggaaaaatt
     4321 actgaaaaag taaccggagt tcttccaaaa aaaaatcagc agattcaatg gttggaatct
     4381 gctgattttt tttcttaaaa gttcttgttt ttttgagtat aaaaaaagga aattctcatt
     4441 tgcagactat atttgcactc gtcattctga taatataaac taaaaaaaac gaattaaaat
     4501 gaaaaaaaca ctcttctcgg tatttgtgct tatgttttta gctgtatcgg catcggcaca
     4561 ggtggataaa attgttggaa aatggaaaac aattgacgat aaagacggaa gcgaaaaatc
     4621 aatagtgaat atatttaaag cttcaaatgg aaaatattat ggtaaagttg aaaaactttt
     4681 caaggaaccc aataaaaaat gtaccgaatg tgaaggtgaa aacaaggata aacccgtact
     4741 tggcatgacc gtactaaaca atatggtaga gaaagacgga acccttacgg gtggtactat
     4801 aatagatccg gccaatggaa aagtgtataa atgcaacgtg agtctggata caaataccgg
     4861 aaaactcaaa gtgcgtggtt cactggataa aagaggagtt atagggcgga ctcagcattg
     4921 ggtaaaaaac tgatattcaa aataatgaaa aaaatatggc ggatctcccg gtttggattt
     4981 tccgccattt tttttatatc agtagtgctt tattttattt tttcgacttg ccggcaagcg
     5041 gaacaaattc agcattcaaa tccggctgag ccatatcttt atccagcggg tacataggcc
     5101 gccggatatt tttgtaggga agtcgagtca aatcctggtc cacacctccc ggagtgagtg
     5161 ccataatcca atcgccacgc atatcataca gttcaggcac cagatagcct attttaacta
     5221 ccagtatgtc tgtttcacgc ggattcagcc ctgcctgtgt gaagtaactt tcataatgat
     5281 aagccttccg ttttttggta acgatcacag agacgctacc tgttttcact acaaccacag
     5341 tgttcccgtc tttttcatcg tttacaatgg cagtaatagt gcctgcaata cgcacaggtg
     5401 gtgcataccg gttatccacc cgggcacctg ctgtggcatc aatttttcct ccgactccga
     5461 ttgcaacagc tttttttaca aatccggaat cgggaataga tgcataaata accgttttcc
     5521 cgttttctga cttaaattca ggtcgtttaa gcagttctgt caacgtccag gtcacatctc
     5581 cggcaccacc ggcagtcgga ttatcgccca tatcgctgat tataaaaggc tttttagtac
     5641 tggccagcgc tttttcgata cattcgggca gatacgcggt aggagccaca aactcaaact
     5701 gatctcgtac attccaaaaa tggttggcaa gtttctcagc tgctttaccc accgcttctt
     5761 tatcgtcgcc gtaagccatc actaccgcat gatttcgggg ctcgtcagcc catggatacg
     5821 acatccagat agcggcatct attacttttt ctccatcaat tacagtagga ataagttcat
     5881 aaaggctttt tgcgggttct atccgggtac ttgttttttc gcccggcagc agtatcggaa
     5941 cagggataat ggctttgaaa gccggttttc ctttgccgtt ttccagccgg tccagcagat
     6001 tggtcaaagc tcttttcttc gaaatcatgg catcctcgtg cggagcaagg cggtagcagg
     6061 taatcagatc cgagttctca gccaggcggg gcgatacact gccgtgtaaa tccatgcttg
     6121 tggaaataag cacttcattc ccaatcactt cgcggatacg tacaatgaaa tctccctccg
     6181 gatcttccaa atcctgcacg ctcatggcac cgtgaatgtc aaagaacaat ccatccagcg
     6241 gaagcgattt tttaagcatt tcaagagtct gatttaccag tttttcgtaa acatccctct
     6301 tcactatgcc tccgggcatg gcgtgagtac gcagtgtagg aagccatt
//
LOCUS       DAORKP010000123         6272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2440_length_6272_cov_7.907029, whole genome shotgun sequence.
ACCESSION   DAORKP010000123 DAORKP010000000
VERSION     DAORKP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2440_length_6272_cov_7.907029"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="PLN75_07320"
     CDS             complement(<1..1381)
                     /locus_tag="PLN75_07320"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HRG03964.1"
                     /translation="MKIYPKIGIRPTIDARQGGVRESLEEKTMNLAKAVANLISSNLR
                     NGDGSPVECVIADSTIGRVGESAACAAKFEREGVGSTISVTSCWCYGSETMDMNPYYP
                     KAVWGFNGTERPGAVYLAAVLAAHAQKGLPAFGIYGHDVQDLDDNSIPEDVAEKILRF
                     AKAAQAVATMRGKSYLSIGSVSMGIAGSIVDPHFFQDYLGMRNESVDSTEILRRMEEG
                     IYDKEEFRKAMEWTEKHCKSNEGTDFNTEAKTKSRKEKNADWEFVVKMTIIIRDLMTG
                     NPTLEKMGFKEEAIGHNAIAAGFQGQRQWTDYKPNADFPEALLNTSFDWNGIREAYVL
                     ATENDALNGVSMLFGHLLTNTAQIFSDVRTYWSPEAVKRVTGKELTGDARNGIIHLIN
                     SGATTLDGTGEQTKDGKPAMKPFWEITPDEADACLKATTWYPANRDYFRGGGYSSNFL
                     SKGEMPVTMT"
     gene            complement(1447..2430)
                     /locus_tag="PLN75_07325"
     CDS             complement(1447..2430)
                     /locus_tag="PLN75_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRG03965.1"
                     /translation="MELHFDHKLTKVKQLADHLQKAITVNDLKAGEKLPSINFLSKKH
                     EVSRDTVFKAFKLLKEIGLIDSNQGKSYFVTAATTDILILLDEYSQFKEALFTSFRNQ
                     LPLTYRVDLLFHQYNENLFNTIIKETAGHYSKYLVMNYNNETFSEALKRIDPQRLILL
                     DFGNFDKQGYSYICQDFDENFYDALVTIKDRLRKYEKLVFVFNKSHKHPKSSKAYFEK
                     FCRDYDFNYLITDEFTENMNVEKGNLYIAIKQSDVVNIIKSFRRENFSPGAEAGIIAY
                     NDNPIYEVVADGISSISIDFRLMGAMASQFVLSGESIQTYLPTNVIIRNSL"
     gene            2797..3225
                     /gene="fucU"
                     /locus_tag="PLN75_07330"
     CDS             2797..3225
                     /gene="fucU"
                     /locus_tag="PLN75_07330"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HRG03966.1"
                     /translation="MLKGISPLLSPDLLAVLCRMGHGDEIVLADAHFPAHAVNPLVIR
                     ADGLRIPDLLAAILPLFELDSYVPNPVVMMAAVKGDELNMTVEKSYMDEINQFYPLIM
                     KPERIERFDFYERAKSAFAVVVTGETAKYGNIILKKGVTP"
     gene            complement(3335..3419)
                     /locus_tag="PLN75_07335"
     tRNA            complement(3335..3419)
                     /locus_tag="PLN75_07335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3383..3385),aa:Ser,seq:tga)
     gene            complement(3651..4361)
                     /locus_tag="PLN75_07340"
     CDS             complement(3651..4361)
                     /locus_tag="PLN75_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG03967.1"
                     /translation="MADRTLIKYENVELCQQEHIVLKDVNIEIMSGEFIFLLGKVGSG
                     KSTFMKSLYAEIPVLDGQANVLDYNLLKLKRKEVPNLRRKIGIVFQDFQLLIDRSINA
                     NLEFVLKATGWKDKNAIAEQIHHVLKQVGMQTKGYKMPHQLSGGEQQRIVIARSLLNS
                     PEIILADEPTGNLDEKTGKDIMDLLYSIRKSGTTVVVATHNLSWIDMYPGRVLVCEEE
                     KLVDITAETISENINQMA"
     gene            complement(4436..5056)
                     /gene="hisIE"
                     /locus_tag="PLN75_07345"
     CDS             complement(4436..5056)
                     /gene="hisIE"
                     /locus_tag="PLN75_07345"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRG03968.1"
                     /translation="MNEEKKDCKMTLDFQKSGGLIPAIIQDSNTNKVLMLGYMSPESL
                     SKTEKTGKVTFFSRSKNRLWTKGEESGNFLHVVSISADCDNDTLLIKVHPEGPVCHTG
                     ADTCWNEENKTDISFLAYLQEFIQKRYDEMPEGSYTTSLFRKGINRMAQKVGEEAVET
                     VIEATNGTDEGFIYEASDLVYHLIVLLTSKGYSLADLAKELEKRHS"
     gene            complement(5187..6224)
                     /gene="asnA"
                     /locus_tag="PLN75_07350"
     CDS             complement(5187..6224)
                     /gene="asnA"
                     /locus_tag="PLN75_07350"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRG03969.1"
                     /translation="MSYFIKPNEYTPLLDPQQTELGIQKIKDFFQANLSAELRLRRVT
                     APLFVLRGTGLNDDLNGIERPVNFAIKDMNDERAEVVHSLAKWKRVTLADYQISDGFG
                     IYTDMNAIRADEELGNIHSLYVDQWDWEQVITEQDRNIEFLKKIVRRIYAAFLRTEYL
                     VSESFPEIKPILPEEITFIHAEELCQLYPSLTPKEREYEITRKHKAVFIIGIGGELSD
                     GQKHDGRAPDYDDWTTPGEDGLPGLNGDILLWNSVLEIPFEVSSMGIRVDKKALQHQL
                     RITGKEDRLELYFHKRLMEDTLPLSIGGGIGQSRLCMFFLRKAHIGEIQASIWPSEMK
                     DECKKDNIHLI"
BASE COUNT         1551 a         1344 c         1385 g         1992 t
ORIGIN      
        1 gagtcatggt tacaggcatt tcacctttcg aaagaaaatt ggatgaatat ccgcctccgc
       61 ggaaataatc acgattggca ggataccagg tcgtagcttt caggcatgca tccgcttcat
      121 cgggtgtaat ttcccagaaa ggtttcatgg ccggtttgcc gtcttttgtt tgctctccgg
      181 ttccgtcgag cgtggtggca cccgaattaa tcaggtggat tatgccgttt ctggcatctc
      241 cggtgagttc ttttccggtc acccgcttaa cggcttcggg actccagtaa gttcgtacat
      301 ctgaaaatat ctgtgcggta ttggtgagca ggtgcccgaa aagcattgaa actccgttta
      361 atgcatcatt ttcggtggcc agtacgtaag cttcgcgtat tccgttccag tcgaatgagg
      421 tgttgagcag tgcttccgga aaatccgcat tgggtttgta gtctgtccac tgacgctgcc
      481 cctgaaaacc cgctgcaatg gcattgtggc ctatggcttc ttctttgaaa cccattttct
      541 caagtgtcgg atttcctgtc atcagatcac ggataattat agtcattttt accacaaatt
      601 cccaatctgc gttcttctct ttgcgcgatt ttgtttttgc ctcggtgttg aaatcggtac
      661 cttcattgct tttgcagtgt ttttcggtcc attccatcgc tttgcggaat tcttccttgt
      721 cgtaaatgcc ttcttccatg cgtcggagta tttcggttga atccacggat tcattgcgca
      781 tgcccaggta atcctggaaa aaatgcggat caacgattga gcctgcaatg cccatcgaaa
      841 cactcccgat ggagaggtat gacttacccc gcatggttgc cactgcctga gctgcttttg
      901 caaatcggag gattttttct gccacatctt ccggaatgga attatcgtcc agatcctgca
      961 catcgtgtcc gtaaatgccg aatgcaggta agcctttctg agcgtgagct gccagtactg
     1021 cagccaggta cactgctccc ggacgttctg ttccgttgaa accccacact gctttcggat
     1081 agtacggatt catatccatg gtctcggaac cgtagcacca gcatgaagtg acggaaatag
     1141 tggatccgac gccttctctt tcgaatttag ctgcgcaggc ggcactttcg cctactcggc
     1201 cgatggtgga gtcggcaatg acgcattcca cagggctgcc atccccattc ctgaggtttg
     1261 agcttataag gttggctaca gcttttgcca gattcatggt cttttcttcg agactttcgc
     1321 gaactcctcc ctggcgggcg tcaatggtgg gacgaattcc gatttttggg tagattttca
     1381 tagtatagtt tattaatgtg attttttaag attttttatg ttcgaaaata tgctgattgt
     1441 tttttattaa agcgaattcc ggataatgac atttgtgggc aaataagttt gaatactttc
     1501 acctgaaaga acgaactgcg aagccattgc gcccataagt ctgaaatcga tgctgatact
     1561 tgaaattcca tcggctacaa cttcataaat cggattgtca ttgtaagcga tgatgcccgc
     1621 ctcagcgccc ggactgaaat tttctctccg gaaacttttt ataatattta ccacatccga
     1681 ttgcttgatg gctatgtata aattaccttt ttccacgttc atattttcgg taaattcatc
     1741 ggtaatgagg taattaaaat cataatccct gcagaatttt tcaaaatagg ctttactgct
     1801 tttcggatgc ttgtgtgatt tgttgaaaac aaaaaccagt ttttcgtatt ttcttaatcg
     1861 gtctttaatg gttaccaggg catcgtagaa gttttcatca aaatcctggc aaatatacga
     1921 gtagccttgc ttgtcgaaat ttccgaaatc gagcagaatt aatcgctgtg gatcgatacg
     1981 cttcagtgct tctgaaaatg tttcgttgtt gtaattcatt accaggtatt tactgtaatg
     2041 tcctgctgtt tctttaatta ttgtattgaa gaggttttcg ttgtattggt gaaaaagcag
     2101 atcgacgcgg tacgtaagcg gaagttgatt tctgaagctg gtgaaaagtg cttctttgaa
     2161 ctgtgaatac tcatccagca gtatcagtat atcggtggtt gcagcggtta caaaatagct
     2221 ttttccctgg ttggaatcaa ttaaaccgat ctctttcagc agtttgaatg ccttgaaaac
     2281 ggtatcgcgc gaaacctcgt gtttcttact caaaaaattg atggaaggca gtttttcacc
     2341 tgctttcaaa tcgttgaccg tgatggcttt ctgtaggtgg tctgccaatt gtttaacttt
     2401 cgtgagctta tgatcgaagt gtaattccat atccaactgt gctgtacttt gctgaatcaa
     2461 aggtatggat gtttagagga aagtaaaaat cattttacat gttgtaaaac ataaaatttg
     2521 ggattttaaa ccaatttaac gcttgaggac tgtaaagttc ggtgaaaata gaatagcttt
     2581 gacgaaaatg taactactta gcccccccca acccccaaaa ggggagtttt ggagcgtcta
     2641 ataagattta cagaatttga aactggaagt tcttttgtaa aaaattataa tttttgtatc
     2701 tgcaattgtt ctgaaaatgc taaattaatt aattatagaa gtgctgagta gtcaccaaaa
     2761 aatcgagtat cattttaaaa aaaataaaac aggaatatgc ttaaaggaat atcacctctg
     2821 ttaagtccag atttattggc tgtgctctgt aggatgggtc atggcgatga aattgtgttg
     2881 gccgacgcac attttccggc tcatgctgtt aatccgctgg taatccgcgc cgacgggctc
     2941 cgcattcccg atttgctggc tgccatcctg ccactgtttg agctggatag ttatgtgccg
     3001 aatccggttg tgatgatggc cgctgtgaaa ggggatgaac tgaacatgac ggtcgagaaa
     3061 tcttacatgg atgagatcaa tcaattctat ccacttatca tgaagcccga aaggattgaa
     3121 cggtttgatt tctacgaaag agcgaaatca gcttttgccg tggttgtaac aggcgaaaca
     3181 gccaaatacg gcaatataat cctgaaaaaa ggcgtcactc cgtgagctga actaatttgt
     3241 tattacagcc tttttgattt cgccgcatta ctgccgcagt aaaaaacaaa aaacgctgta
     3301 tattattgat atacagcgtt ttacatacat tttgcggaga gagagggatt cgaacccccg
     3361 gaacctctca gttcaacggt tttcaagacc gccgcgatcg accactctgc catctctcca
     3421 tggtttcatt tctcaaaagc gactgcaaag atactgcatt ttttcaaata tcaaaatatc
     3481 tcactcaata tttgacaaaa agtaaataaa tacttgtacc ttgttttttg ggaaaaggga
     3541 tagaaaatca gaacttagaa tgacatatac gtacagcaga tgacaccata ataaaaaacc
     3601 ggaaagcaaa actgctgccc ggccctggag aattcttttc aatggtagtt ttatgccatt
     3661 tgatttatgt tttcggagat ggtttctgct gtgatatcca ccagtttttc ctcttcgcac
     3721 accaataccc tgccgggata catgtctatc cagctcagat tatgggttgc tacaaccacg
     3781 gttgtgcccg attttcggat agagtagagt aaatccatga tgtcttttcc tgttttttcg
     3841 tcgagatttc cggtgggttc gtctgccagg attatctcgg gtgaattgag cagcgaccgg
     3901 gcaatcacaa tacgttgctg ctcgccccct gatagctgat ggggcatttt gtagcctttt
     3961 gtttgcatac ctacctgttt cagtacgtga tgtatttgtt ctgcaatggc gtttttgtct
     4021 ttccagccgg tggctttgag cacaaattcc agattggcat tgatgctgcg gtctatcagc
     4081 agctgaaaat cctgaaatac aatgcctatt tttcgtctca gattgggaac ttctttccgt
     4141 tttagtttca acaggttgta atcgagcaca ttggcttggc cgtctaagac gggaatttcg
     4201 gcgtaaagcg atttcataaa cgtgcttttt ccgcttccca cttttcccaa cagaaatatg
     4261 aattcgccgc tcataatttc aatgttgaca tccttcagca cgatgtgttc ctgctgacat
     4321 agctctacat tttcgtattt tataagtgtt ctgtctgcca tttttcagat ttttgatatt
     4381 ggatacagga aatcagacat gtattatatg tttttgattt cagtttttta tcgtgttatg
     4441 aatgtctttt ttcaagttct ttggccaaat cggcaagcga atagcctttg gaggtgagca
     4501 gtacgataag atgatacacc agatctgacg cttcgtaaat gaatccttca tcggttccgt
     4561 ttgtagcttc tataacagtt tcaactgcct cttcgccaac tttttgtgcc atcctgttga
     4621 tcccttttct gaaaagcgag gttgtatagg agccttcggg catctcatcg tagcgctttt
     4681 ggatgaattc ctgcaggtat gccagaaacg aaatgtctgt tttattttcc tcgttccagc
     4741 aggtgtcggc tccggtgtgg caaaccggtc cttcaggatg aacctttatc agaagtgtat
     4801 cgttgtcgca gtcggccgaa atggacacta cgtgcagaaa attgccactt tcttcgcctt
     4861 tggtccagag tctgttttta cttcggctga aaaatgtgac ttttccggtt ttttcagtct
     4921 tgctcaatga ttcagggctc atgtatccga gcatcaacac tttattggta ttgctgtcct
     4981 gaatgatggc aggtatcaat ccgcctgatt tttgaaaatc caacgtcatc ttgcagtctt
     5041 ttttttcttc gttaataaat ggcgaaagta ctaaaaatcg tccaaccgga caattattaa
     5101 gtattaaaaa acaaaaaacc ctgaatggag gtggtctctt tcagggtatt tatttcatgt
     5161 ccggattttt caggatttat tatctgttaa atcaaatgaa tattatcttt tttgcattcg
     5221 tctttcattt ccgatggcca gatgcttgcc tgaatttcgc cgatatgtgc cttccgcagg
     5281 aaaaacatac aaagacgcga ttgaccaatg cctccgccaa tggaaagtgg gagtgtatcc
     5341 tccatcagtc gtttgtggaa atagagttcc agccgatcct ctttgcccgt aatccgaagc
     5401 tgatgctgca acgctttttt atctacccgg atacccatgg atgacacttc gaacgggatt
     5461 tccagcacgc tgttccatag cagaatgtcg ccgttcagtc ccggcagtcc gtcttcgccg
     5521 ggcgtagtcc agtcgtcgta gtcgggtgcg cgtccgtcat gcttttggcc gtcgctcagt
     5581 tcgccgccga ttccgataat gaaaactgct ttgtgcttcc tggtgatttc gtattcccgt
     5641 tctttgggag tgagtgaggg ataaagctgg cagagttctt ctgcatggat aaatgtgatt
     5701 tcttcgggta aaatcggctt tatttccgga aaactttcac ttacaaggta ttctgtccgc
     5761 agaaatgcgg cgtaaattct tcgaactatt tttttaagaa actcaatatt ccggtcctgt
     5821 tcggtaatga cctgttccca gtcccactga tcaacgtata gcgagtggat gttgccaagc
     5881 tcctcgtcgg cacgaatagc gttcatgtct gtgtaaatgc cgaatccatc gcttatctgg
     5941 taatcggcca aagtcactcg tttccatttg gcgagcgaat gaaccacttc ggcccgttcg
     6001 tcattcatat ctttgatggc aaaattcacg ggtcgctcta tgccgttcag gtcgtcgttg
     6061 agcccggtgc cccgcaaaac gaaaagtggt gccgtgacgc ggcgtaagcg caattcggcc
     6121 gataaattgg cctggaagaa atcttttatt ttttgtattc ccagttctgt ctgctgaggg
     6181 tcgagcaggg gagtgtactc gttgggtttg ataaaatagc tcatgtgtga ttttgtttga
     6241 taatgtgtga aatagattgc aaaaatacaa ct
//
LOCUS       DAORKP010000124         6199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2502_length_6199_cov_8.737142, whole genome shotgun sequence.
ACCESSION   DAORKP010000124 DAORKP010000000
VERSION     DAORKP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_6199_cov_8.737142"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..757)
                     /gene="aqpZ"
                     /locus_tag="PLN75_07355"
     CDS             complement(56..757)
                     /gene="aqpZ"
                     /locus_tag="PLN75_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HRG03970.1"
                     /translation="MKKNLAEFLGTFGLVLGGCGSAVLAAGIPDLGIAYAGVALAFGL
                     TVLVGAYGLGHISGAHFNPAVTLGLYAGGRTSGKDVLPYIISQILGALAAAAVIYIVA
                     SGKAGFAIDNSQPGAFAANGYGALSPHGYGVLSAVVIEFVLTFMFLIVIMGATDERAP
                     KGFAGLAIGLALTLIHLISIPVTNTSVNPARSISQAVFAGGGYIPQLWLFILVPIAGG
                     MCAGWVYKSFLSEKK"
     gene            1056..4388
                     /gene="mfd"
                     /locus_tag="PLN75_07360"
     CDS             1056..4388
                     /gene="mfd"
                     /locus_tag="PLN75_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRG03971.1"
                     /translation="MNLSEFQHLYAHHPQIEALTLWAATHESNLRISGLCGSSLSLVM
                     AAFTQRVKQNHVFVMTDADEAGYLYNDLRQILSDEVVMFFPSSYKKTIRLSQLDSSNE
                     ILRTEVLNRLTNTSSQAIVVTYPEAMMQKVISQHGFKSKTLKIKAGENISIGFLIEIL
                     TAYGFQRVDFVYEPGQFSVRGGIVDVFSFAFELPYRLDFFGDEVETIRIFDIETQLSQ
                     DKKQQIEIVPDLHKSYNDTKYVSFPEFVSGQTWFHFTDVAFVRDRINQLYDDALVKAN
                     TENKLVENLYATHITGHEFLQHLTDYRKPEWGNKSFFKAKQTIHFNTSPQPVFQKNFE
                     LISNNLKKNIAEGYKIFIFSDSVKQTDRIAAIFKDRNDEIVFQPVKNTIHEGFVDHDL
                     SLVCYTDHQIFDRFHKYQLKTDKTRQGKVVMTLKELNQLQFGDFVVHVDHGVGKFGGL
                     VRTNVNGKMQEMVKLIYKDDDIIFVSIHSLHRISKYKGKEGEAPRINKLGSGAWERLK
                     ERTKAKVKDIARDLIKLYAKRKAEKGFEFSPDSYLQQELEASFLYEDTPDQVKATADI
                     KKDMETDMPMDRLVCGDVGFGKTEVAIRASFKAVADSKQVAVLVPTTVLAMQHYNTFK
                     DRLKDFPCTISYLSRAKSAQKTSETLKKLEEGKIDIIIGTHKLVGKSVKFKDLGLLII
                     DEEQKFGVSVKEKLKELKVNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRYPV
                     QTEIHPFDEEVIRDAIQLEMSRNGQVFFINNRIQNIYMIEAMIKRLVPGCRVAVGHGQ
                     MHADKLEEIIMDFIDYEYDVLVATTIIESGIDIPNVNTIIINSAHRFGLSDLHQLRGR
                     VGRSNRKAFCYLLSPDMSLLTPEARRRLKAIETFAELGSGFNIAMQDLDIRGAGNMLG
                     AEQSGFIADLGYETYQRILNEAVHELKDEEFADLYADERTEGGKNKVYVTDCQIDTDM
                     ELMFSADYIENVSERISLYQTLDALESESELERFQRELTDRFGKIPKQATGLMLIVRL
                     RWLAMRYGVEKLVLKNQQMIAYLVSNQKSVYYQSDEFGRMLQYMARNPRVCKLREQNG
                     RRSVVFSTVKSVEEAWGVFEAI"
     gene            4827..5501
                     /locus_tag="PLN75_07365"
     CDS             4827..5501
                     /locus_tag="PLN75_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG03972.1"
                     /translation="MSKKQEAKVEELESVGRALSNSEAFIEKNQKPIFIGVGVVVLIV
                     LGIMAFHNFYLKPRETAAENAMYRAQEFFAVDSFRVALEGDGTKSMGFKEIASEYGMT
                     ASGNLANAYTGICYYKLGKYEDAIKYLVQYDGDDNYFKTSVVGLTGDCYAELGDKGKA
                     QSFYKKAIAEKNELSPIYLKKSGILFETDGKAAEAEKAFQQIKDDYPMSNEAYDIDKY
                     LARVQK"
     gene            5584..6105
                     /gene="ribH"
                     /locus_tag="PLN75_07370"
     CDS             5584..6105
                     /gene="ribH"
                     /locus_tag="PLN75_07370"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRG03973.1"
                     /translation="MATQYQNLSDYDSELMPDKQTVENQSYAIVVADWNPDITNSLLN
                     GAMSTLTENGVLSKNIKVFHVPGTFELTHGAAKLMNQYKTVSGKKKRLYDAIIVIGCV
                     IQGETPHFSYVCSSVTKGVTELNLIPDSCPVIFGVLTTNTLQQAKDRAGGIHGNKGDE
                     AAITAIKMANIVW"
BASE COUNT         2010 a         1268 c         1283 g         1638 t
ORIGIN      
        1 gcgtgttcca tacgtcctta atttaaaaaa ataaaaaatc ttcgtatgaa aaaaatcatt
       61 tcttttcgct cagaaaagat ttataaaccc atccggcaca catgccccct gctatgggca
      121 ccagaatgaa aagccacaac tgtggaatgt atcctccacc ggcaaatacc gcctgagaaa
      181 tactgcgggc aggatttaca gaagtgttgg ttaccggaat actgatgagg tgaatgagag
      241 tgagcgccag accgatggct aatccggcaa aacctttggg agcgcgttcg tcagtagcac
      301 ccatgattac tatcagaaac atgaaagtaa gaacaaactc aatgacaact gccgacaata
      361 ctccataccc gtgaggtgaa agagcgccat atccgttggc agcaaaagca cccggctgag
      421 agttgtcaat agcgaatcct gctttgccgg aagctacaat gtaaataact gctgcagcgg
      481 caagggctcc aagaatctga gaaatgatgt agggcaatac atctttaccg ctggttctgc
      541 caccggcata aagacccagg gtaacggcag ggttgaagtg agctcctgaa atatgcccca
      601 ggccgtaggc acctaccagt acggtaagac cgaatgcaag ggctactccc gcataagcaa
      661 tacctaaatc gggtattcct gctgccaata cagcactacc acaacctcca agtaccaaac
      721 cgaaagttcc gagaaattct gctaagtttt tcttcataaa attaatatgt taatttatac
      781 ctactcgtaa ggcttttcgg tatccgcctc ctgaataact tatttttttt ttttgaaaaa
      841 aaagttgatg aatgtcaaaa gtagaaataa tttttgatta taataactat ttaacatatt
      901 ttcaacttgt tatccgaaaa aaaaaacaaa gtgaaatttc tacgatagcg taatactgat
      961 aatatttctg tttaattttt caggttttca atttacaaat gaatcttcct atatcatgta
     1021 aaatgattat ctttgcaacc cattttttac tgattttgaa tttatccgaa tttcaacatc
     1081 tttatgccca ccatcctcag atagaagcgt tgactttgtg ggcagcaaca cacgaatcaa
     1141 atctcagaat ttccggtttg tgcgggtcgt cgttgtcact tgtaatggct gcattcacac
     1201 agcgggtaaa acagaatcat gtgtttgtga tgaccgatgc cgacgaagca ggatatctgt
     1261 ataacgacct ccggcagata ctatccgatg aggtagtcat gttttttcct tcgtcataca
     1321 aaaaaaccat tcgcttgtcg cagctcgatt cttccaacga aatactccgg acagaagtgc
     1381 tcaaccggct gactaacact tcttcacagg caatcgtagt tacctatcct gaagccatga
     1441 tgcaaaaagt gatttcacag catggtttca aatcgaaaac attgaaaata aaagccggcg
     1501 agaatatcag tatcggtttt ctgatagaaa ttttaacagc gtatggcttt caacgtgtcg
     1561 atttcgtgta cgaaccgggt cagttttcag tacgtggagg cattgtggat gttttttcat
     1621 ttgcattcga actgccatac cgacttgatt tctttggcga tgaagtggaa acaatccgaa
     1681 tttttgatat tgaaacacaa ctttcgcaag ataaaaagca gcaaatagaa attgtgcccg
     1741 atttgcacaa aagctacaat gatacaaaat atgtcagttt tccggaattt gtgtccggtc
     1801 aaacctggtt ccattttaca gatgttgcct ttgtccgcga ccggatcaat cagctttacg
     1861 acgatgcttt ggtaaaggcc aatacggaaa ataaactagt tgaaaatctg tatgccacac
     1921 atatcacagg ccatgagttt cttcagcatc ttacggatta ccgaaaaccg gaatggggaa
     1981 ataaatcttt tttcaaagcc aaacaaacca tccatttcaa tacttctcca cagcctgttt
     2041 ttcagaagaa tttcgaatta ataagcaaca acctgaagaa aaatatcgct gaaggttata
     2101 aaatctttat tttcagcgac agcgtaaaac aaaccgaccg gattgctgcc atctttaaag
     2161 accgaaacga tgaaattgtt tttcaacctg taaaaaacac cattcacgaa ggttttgttg
     2221 atcatgatct ctcattggtt tgttacaccg accatcagat ttttgaccgt tttcacaaat
     2281 atcagctcaa aaccgataaa acacgccagg gtaaagtggt gatgacactc aaggaactca
     2341 atcaattaca atttggcgac tttgtggttc atgtagatca tggagtaggc aagtttggcg
     2401 ggctggtaag gactaatgtg aacgggaaga tgcaggaaat ggtgaaactg atttacaaag
     2461 atgacgatat aatatttgta agtattcatt cgttgcaccg gatttcaaaa tacaaaggca
     2521 aagaaggcga ggcaccccgt atcaacaagc tcggctccgg tgcctgggaa aggctgaaag
     2581 aacgcacaaa agccaaagtt aaggacatag cccgcgacct gattaagctc tatgccaagc
     2641 gaaaagccga aaaaggattt gaattttcgc ccgacagtta tttgcagcag gagctggagg
     2701 cttcttttct gtatgaagat acacccgatc aggtgaaagc taccgccgat atcaaaaagg
     2761 atatggaaac cgatatgcct atggacaggc ttgtatgtgg cgatgttgga tttggtaaaa
     2821 ccgaagttgc cattcgtgca tcgttcaaag ctgtagccga cagcaagcag gtggctgtac
     2881 ttgtgcccac tacggtactt gcaatgcagc attataatac tttcaaagac aggctgaaag
     2941 attttccgtg caccatttca tacctgagcc gagccaaatc tgcccagaaa acttcagaaa
     3001 cgcttaaaaa actcgaagaa ggaaagattg acattatcat cggtacacat aaactggttg
     3061 gtaaatcggt aaaattcaaa gatttaggtt tgcttatcat tgacgaagag cagaagtttg
     3121 gtgtatcggt aaaagaaaaa ctgaaagaac tcaaagtgaa tgtggataca ctcactctca
     3181 ctgctacacc cattccccgg acgcttcagt tttcgctgct tggtgcccgc gatttatcaa
     3241 tcatcaatac accaccacca aacagatacc ctgttcagac tgaaattcac ccattcgacg
     3301 aagaagttat ccgcgatgcc atccagctgg agatgagccg taatggccag gtttttttta
     3361 tcaataaccg aattcagaat atttacatga ttgaggccat gataaaaaga ctcgttccgg
     3421 gttgcagggt agctgtaggt cacggacaaa tgcatgcaga taaactggaa gaaatcatta
     3481 tggatttcat tgattatgaa tacgatgtgc ttgtggcaac caccatcatc gaatcgggca
     3541 tcgacattcc gaacgtaaac accataatta tcaatagtgc tcaccggttt ggactaagtg
     3601 acttgcatca gttgcgcggc agggtagggc gcagcaaccg gaaagccttc tgttacctgc
     3661 tttcgccgga tatgagtctg ctcacacctg aggcacgcag gaggctcaaa gccatcgaaa
     3721 cctttgccga acttggaagc ggattcaata tcgctatgca ggatctcgat attcgtggag
     3781 ccggcaatat gctgggtgct gagcagagtg gttttattgc cgatttgggt tacgaaacct
     3841 atcaaagaat tctgaacgaa gccgtacacg aactcaaaga cgaagaattt gccgatctgt
     3901 atgccgatga acgcaccgaa ggaggtaaaa ataaagtata tgtaacagat tgtcagattg
     3961 atacagatat ggaactgatg ttttcggcag actacatcga aaatgtttcg gagcgtatct
     4021 cgctgtatca gacactggat gctctggaat cagaatccga acttgaacgt tttcagcgcg
     4081 aactgaccga ccggtttgga aaaataccca aacaagccac cggattgatg ctcatagttc
     4141 gactgcgctg gctcgccatg cgttacggcg ttgaaaaact ggtattaaaa aaccaacaga
     4201 tgattgcata cctggtcagc aatcagaaat cagtctacta tcagtccgat gaatttggac
     4261 gcatgcttca gtacatggcc agaaatccac gtgtatgcaa actcagggag caaaacggtc
     4321 gacgctcggt tgttttttca actgtaaaat cggtagaaga agcctgggga gtgtttgaag
     4381 caatataaat attacctgaa aattttgata agctaaaaga aataaaacga ttcaacaatg
     4441 caaaggtatt taaaataaaa aaccgcattg ttacctgttg agaaaactcc tataaatagg
     4501 atttgaggca tcggttttct ttgtaatctg ctgtcccgac gtatcggaat acccgaaggt
     4561 gcagcccgcc attgaaaacc tttgtatctc ggaataaatt atctgttgag ttttccaatc
     4621 tctgaacaat gcggttcaca aatataatac ttttaaatga aatttgtttc ggcattatga
     4681 taattttttg ttaaaactta agacaaataa cactgtgatg ctgtaaatca aaaatgtagc
     4741 aaaaaatagc cttaatattt cgctatatgg aagaaaaatc ttacttttgt ggttcaaaaa
     4801 aataaaacag atataaacca ttcaatatgt caaagaaaca agaagcaaaa gttgaagaac
     4861 ttgaaagtgt cggacgtgct ctgtccaatt cagaagcttt tattgaaaaa aatcaaaaac
     4921 caatctttat cggagtagga gttgtagtat taatcgtatt gggcattatg gcgtttcata
     4981 atttctatct taaaccacgt gaaacagctg ccgaaaatgc catgtacagg gcacaggaat
     5041 tttttgccgt tgattcattc agagtagctt tggaaggtga cggtacaaag tcgatgggct
     5101 tcaaggaaat tgcatccgaa tatggcatga ctgcaagcgg caacctggca aatgcatata
     5161 caggtatttg ttactataaa ttaggtaaat acgaagacgc tatcaaatac ctggttcaat
     5221 acgacggaga tgataattac ttcaaaactt cggtagtcgg tttgaccggc gattgctatg
     5281 cagaactcgg cgataaaggg aaagcacaaa gtttctataa aaaagccatt gccgaaaaaa
     5341 atgaactcag tcccatctat ttgaaaaaat caggcatact gttcgaaact gacggaaaag
     5401 cagctgaagc tgaaaaagca tttcagcaaa tcaaagacga ctatccgatg agcaacgaag
     5461 cttatgacat tgacaagtat ctggcccggg tacaaaaata aatttcacat aaaaataaaa
     5521 ccaaagaaag ctgctttcag tgtatgaatg cagttttttt tagtcttaaa aaatttacaa
     5581 cttatggcca cacaatatca aaacctttct gattatgatt cggaattaat gcccgacaaa
     5641 caaacagttg agaatcaatc ctatgccata gtggtagccg actggaatcc cgatattacc
     5701 aattcgctgc tgaatggtgc catgtcaacc ctgactgaaa acggtgtgtt aagtaaaaat
     5761 ataaaagtat ttcatgtacc cggaacattt gagctgactc atggtgctgc taaactcatg
     5821 aatcaataca aaaccgtgtc aggtaagaaa aaacgtcttt acgatgccat cattgttatc
     5881 ggatgtgtta ttcaaggtga aacccctcat ttttcatatg tatgcagcag cgtgaccaaa
     5941 ggtgtgactg agctgaatct gatacccgat tcttgcccgg ttatcttcgg agtgctcact
     6001 accaatacat tacagcaggc aaaagaccgc gccggcggaa tacacggtaa caaaggtgac
     6061 gaagctgcca taacagcaat aaaaatggca aatatagttt ggtaattcaa tcataaaacc
     6121 ttatctttgt acccgcaaat taacaaaggg cagttaccaa agtggccaac tggggctgac
     6181 tgtaactcag ctgtctttc
//
LOCUS       DAORKP010000125         6170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2525_length_6170_cov_8.108749, whole genome shotgun sequence.
ACCESSION   DAORKP010000125 DAORKP010000000
VERSION     DAORKP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_6170_cov_8.108749"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="tsaA"
                     /locus_tag="PLN75_07375"
     CDS             complement(<1..666)
                     /gene="tsaA"
                     /locus_tag="PLN75_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRG03974.1"
                     /translation="MNPNQPHRPKLPLQGAGGPVIARIRTDFPTKFGIPRQSGLVKEL
                     QGRIVFEPEYRNPEAVRGLEDFSHVWLIWEFSESKRDGWSPTVRPPKLGGNIRKGVFA
                     TRSPFRPNPIGLSAVKLEKVDIHPELGPVLHVSGADLMDGTPIYDIKPYIPYADCIPD
                     AKGGFTRETDNSLLEVIFPENLLEIIPFEKQKAVMQILAFDPRPSYQEDDERIYGVEF
                     AGFD"
     gene            complement(815..2995)
                     /locus_tag="PLN75_07380"
     CDS             complement(815..2995)
                     /locus_tag="PLN75_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRG03975.1"
                     /translation="MKHLLSIILFLSVFHFASADLLTDITDGKYRAKTVSGIKSMNDG
                     EHYTLLQNNKLIVKYSYKTGQAVDTIFDASTTKLNKIEQIRGYDFSPNEQKILVYNEQ
                     KFRYRRTFTSEYYVFDVKRNELKPLSGNGPQEVPLFSPDSRYIAFARENNLFLHKLDF
                     GTESPITKDGEFGKVINGTPDWVYEEEFVNTRYFVFSPDSKLVAFVKFNETDVPTYQM
                     QKYLQSSLMQDGMPLYPVFQSFKYPKPGQPNSGVSVCVYDDFYKSVRTVNLGTIETDF
                     YIPRIKWTKEQDKLAVFVLNRNQNRLDMIYAHPKSLVSKLILREEDKYYVDYDIIDDI
                     YFTGDGKYFTFVSERNGWRHAYLYNMNGTLSKALTQGNFDVTKVYGYDETKQTLYFQS
                     AEQSPLQRDVYAVDAKGKKTRLTDGKGTHNALFSSTFAYFSDDASGLNSPNVTTMRNS
                     KGESIRILEDNANLVAEFKSLNLPAKEFFSFTTSEGILLNGWMIKPVNFDSQKKYPAL
                     MTQYSGPNSQEVKDSWNAGWEYYLASQGYVVASVDGRGTAARGAQFRKCTYMQLGVLE
                     TKDQVEAAKYLGKQSFVDKNRIGIWGWSYGGFMTLNAMSTGEKIFKAGIAVAPVTDWR
                     LYNSAYTERFMRQPKENNGGYDAGSPIKHAEKLNGEVLIIHGTADDNVHVQNTYLYLD
                     ALLKADKQAELYLYTDKNHSILGKETRRHLYQKKFEFLESELKN"
     gene            3280..4128
                     /locus_tag="PLN75_07385"
     CDS             3280..4128
                     /locus_tag="PLN75_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRG03976.1"
                     /translation="MKSIIELFSHPAPHESKYKIGISLSGGGALGFAHIGVLQALEDY
                     RIFPEAISGSSIGSIVGAMYAAGYTPTQMLQFIKDGKLYKITNIMNFQPTFWKAGLSN
                     HNSILSMMKEFIPNNSFDKLEKKLFVCVSNLTTGNWEIISKSEKLDIWIAASCSIPGI
                     FNAMKINDMMYVDGGLLNNLPSQPLRDRCEFVIGSDVIPHLESKKVLKSRDTLVESLR
                     VALHQNSLAGRTKCDYLIESEAIEKHHEFNYDAYQSIYQSGYRAASQFISNNPDILKL
                     QSPVKS"
     gene            4180..5157
                     /locus_tag="PLN75_07390"
     CDS             4180..5157
                     /locus_tag="PLN75_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRG03977.1"
                     /translation="MSELFLFKLFGNLEQLDKIRLNFSDGGVLFMNFAIAFVMFGVAL
                     GIKTEHFKDLAKNPKTIVLGVISQYFILPAVTFLLVLLINPSPAVAFGMILVAACPGG
                     NVSNMISMLAKGNVALSVSLTAFTTVFALFMTPFNFTFWGGLYARQSPYLVELVIEPS
                     QIFQTVGLILGIPVLLGLIVGARFPVFREKIQKPVRWASMFVLIGFIVGALAANFDHF
                     MKYIHLVFFIVLIHNALGFTVGYWFPKLFKENSQNSRTIAIETGIQNSGLGLALIFNP
                     KIFPPELELGGMAFIAAWWGVWHIVAGSGLAFYWGRRPSKSAKRDFAEF"
     gene            5478..>6170
                     /locus_tag="PLN75_07395"
     CDS             5478..>6170
                     /locus_tag="PLN75_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRG03978.1"
                     /translation="MQLHVNRVFRLHFHKLVFTGKENIPQDKPLIYAPTHRNALMDAL
                     ILVHENVKQQVVFLARADIFKKPLVIKILYLFKIMPVYRIRDGKENLGKNKEIFVQCG
                     EILKNGNPLCLFPEARFNPHQNLLPLQKAIPRIALPTEAETGFTLDTHIVPVAFYYTA
                     KDRFLSDLYVTYGKPVRVADYKELYLTDENQAINKLRNDLDDRLRQYVVDIPEEDYGE
                     ISALIDVNANDLA"
BASE COUNT         1660 a         1293 c         1316 g         1901 t
ORIGIN      
        1 atcaaaaccg gcaaattcca ctccgtaaat tcgctcatca tcctcctgat acgatggtct
       61 ggggtcaaat gccaaaattt gcatgactgc tttttgtttt tcgaaaggaa taatctccaa
      121 taaattttca ggaaaaatga cttccaataa actattatct gtttcccgtg taaaaccacc
      181 tttggcatca ggaatacaat cagcgtatgg aatataaggt ttaatgtcgt aaatcggcgt
      241 tccgtccatc agatctgctc ccgaaacgtg caaaacagga cccagttcgg ggtgaatatc
      301 taccttttcg agttttacag ccgaaagccc gatgggattg ggtctgaaag gcgagcgggt
      361 ggcaaaaacg cctttgcgga tatttccacc aagcttcgga ggtcgcaccg taggcgacca
      421 gccatcgcgc ttcgattccg agaattccca aatcagccac acgtgcgaaa aatcttccaa
      481 tccacgaacc gcctccggat tgcggtattc cggttcaaac acaatcctgc cctgcaattc
      541 cttcaccaat ccactctgac gcggaatgcc gaattttgtg ggaaaatcag tgcggatacg
      601 ggcgataacc ggccccccag ccccctgaag ggggagcttt ggtctgtgtg gttgattggg
      661 attcaatgat tgttagattg ttagtttatt cttttggatg tttggatgtt tggatgttta
      721 ttagctaatt tttcgtcctt cgtgctttca accaacttca gcgaaattag ttttaattcg
      781 tgtaattcgt cttaattctt cttaattcgt ataattagtt cttcaattca ctctccaaaa
      841 actcaaactt tttctggtac aaatgtctgc gggtttcttt gccgagaatg ctgtggtttt
      901 tatcggtata aagataaagt tcggcttgtt tatcggcttt tagcaatgca tccaggtaaa
      961 gatacgtatt ctgcacatgc acattgtcgt cggcagtacc gtgaataatc agtacttctc
     1021 cgttcagttt ttctgcatgt ttgatgggtg aaccggcatc gtatccgccg ttgttttctt
     1081 tcggttgacg cataaaacgc tcggtgtaag cagaattata cagtcgccag tcggttacgg
     1141 gcgcaacagc aattcctgct ttgaaaatct tttcgccggt gctcattgca ttcagcgtca
     1201 taaatccgcc gtagctccag ccccagattc cgatccggtt tttatctaca aaactctgtt
     1261 ttcccaggta ttttgccgct tccacctggt ctttggtttc caaaactccc agctgcatgt
     1321 aggtgcactt tctgaattga gctccccgtg cagccgtacc tcgtccgtcc acgcttgcca
     1381 ctacataacc ttgtgatgcg agataatatt cccagcctgc gttccagctg tctttcactt
     1441 cctgtgaatt tggtccgctg tattgcgtca tcaaagccgg atatttcttt tgggaatcaa
     1501 aattcacagg tttaatcatc cagccgttga gcaatatacc ttctgaagtg gtaaaactga
     1561 aaaattcttt tgcaggcaaa ttcaaacttt tgaattcagc aacaaggttg gcattatctt
     1621 ccagaatccg gattgattca cctttgctgt tccgcatcgt tgtaacattt ggactattca
     1681 ggccggatgc atcgtcggaa aaataagcaa aagtggaaga gaaaagtgca ttatgagtgc
     1741 ctttgccatc tgtaagccgg gttttctttc cttttgcatc cacagcatac acgtcgcgct
     1801 gcaatggcga ttgttcagca gactggaagt aaagtgtttg tttggtttcg tcgtatccgt
     1861 acactttggt tacatcaaag ttgccctgag tgagcgcttt gctgagggta ccattcatat
     1921 tgtaaagata cgcatgtcgc catccgttac gttcgcttac gaaagtgaaa tatttaccgt
     1981 caccggtgaa atagatatcg tcaataatat cgtaatcaac ataatatttg tcttcctccc
     2041 ttagaatcaa tttcgaaacg agtgatttcg gatgagcgta aatcatatcg aggcggtttt
     2101 gattgcggtt gagcacaaat acagccagtt tatcctgttc tttggtccat tttattcgag
     2161 gaatataaaa atccgtttca atagttccta aattgacagt gcgcacagat ttgtaaaaat
     2221 catcgtacac gcatacgctc acgcctgaat ttggctgacc gggtttggga tatttaaacg
     2281 actgaaaaac aggatataaa ggcattccat cctgcatcag ggagctttgc aggtactttt
     2341 gcatctgata ggtgggaaca tccgtttcgt taaatttcac aaaagccacc agtttactgt
     2401 cggggctgaa aacaaaatag cgggtattca caaattcctc ttcatagacc cagtcgggag
     2461 tgccgttgat tacttttcca aattcgccgt ctttggtgat gggactttcg gtgccaaaat
     2521 ccagcttatg cagaaacagg ttattctcgc gtgcaaaagc gatgtaccgg ctatcgggtg
     2581 aaaaaagcgg aacctcctgc ggaccatttc ccgaaagggg tttcaattcg tttcttttca
     2641 cgtcgaacac gtaatattct gaagtaaaag tgcggcgata acggaatttt tgctcgttgt
     2701 aaaccagaat tttctgctcg ttcgggctaa aatcgtatcc tctgatctgc tcaattttat
     2761 tgagtttggt agttgatgca tcgaaaatcg tatcgactgc ctgcccggtt ttataactgt
     2821 actttacgat cagtttattg ttttgaagca gtgtataatg ttcgccatca ttcatggatt
     2881 tgataccgct gacggttttt gcacggtatt ttccatcggt gatgtccgtc agcaaatcgg
     2941 cagatgcgaa atgaaaaacg gaaagaaaaa ggatgattga aagtagatgt ttcataagaa
     3001 atgacgcttt aaccctgcct gcagcaggcc ctgaatggga taagatttta taatttaacg
     3061 atttacaatt tgagaaaaga aacattttag ataattcaaa aaaatgctct tgcaatttac
     3121 caattactcc ccccggatca ctgagcgaag tcgaagtgca ggggttgtgg gtgtaaaatt
     3181 acctctattt cccaattatt tcattttatt tggcaaaaaa tttatttcca ttaacacttt
     3241 tgagttatat ttgcataaca aaaaaaaaca tcagttggta tgaaatcaat tattgaactg
     3301 ttttcgcatc ctgcgcctca tgaatcgaaa tacaagattg gtatttccct gagtggcggt
     3361 ggtgcattgg gatttgctca catcggggtg cttcaggcgc tcgaagatta ccggattttt
     3421 cccgaagcta tctccggttc aagtattggc agtattgttg gtgctatgta tgcagccgga
     3481 tatacgccta cacagatgct gcagtttatc aaagatggca aactttacaa gattacgaat
     3541 atcatgaatt ttcagcctac tttctggaaa gccggattat ccaatcacaa ctcgatttta
     3601 tcgatgatga aggaatttat tccaaacaac agttttgata aactcgagaa aaagttgttt
     3661 gtgtgtgtct caaatctcac aactggtaat tgggaaatta tttcgaaaag cgaaaagctg
     3721 gatatctgga ttgcggcttc gtgtagcatt ccggggatat tcaacgccat gaaaatcaac
     3781 gatatgatgt acgttgacgg cggattgctc aacaacttac cctctcaacc gctcagagac
     3841 cggtgtgaat ttgtgattgg ttctgatgtg atacctcatc tggagtccaa aaaagtactg
     3901 aaatcacgtg atacactcgt ggagtcgttg cgggtagcgc ttcaccaaaa ctcattagcc
     3961 ggcagaacca aatgtgatta tctgattgaa tcagaagcta tcgagaagca ccacgaattc
     4021 aattacgatg cctatcaatc catttatcag tcgggttaca gagctgccag tcaattcatt
     4081 agcaacaatc ccgacatttt aaaactacag tcgccggtga aatcatagtg tatcagattc
     4141 gacgtgtaca agaattaaag aatcattaaa acaaggaata tgagcgaatt atttctattc
     4201 aaattgtttg gtaatttaga acaactggat aaaatccggt tgaatttcag cgatggcggc
     4261 gtgcttttta tgaattttgc catcgctttt gtaatgtttg gtgtagcgct cggcatcaaa
     4321 accgaacact tcaaagatct ggctaaaaat ccaaaaacca ttgttctggg cgttatatcg
     4381 cagtatttta tacttccggc agtaacattt ttactggttt tgctgataaa tccatcgcct
     4441 gcagtggcat ttggtatgat actggtggca gcctgtccgg gcggaaatgt ctcaaatatg
     4501 atttcgatgc ttgccaaggg aaatgtggcg ctttcggtga gtcttacagc atttactacg
     4561 gtttttgcgc tgtttatgac accttttaac tttacattct ggggcggact ttatgctcgg
     4621 caatcgcctt atctcgtgga actggtcata gaaccatctc agatttttca gacggtgggt
     4681 ttgattctgg gtatccctgt tttactgggt ttgatagttg gagcgcgatt tccggttttt
     4741 cgcgaaaaaa tacagaaacc ggttcgctgg gcttccatgt ttgtgctgat tggatttatt
     4801 gtcggagcgc ttgcggctaa ttttgatcat ttcatgaaat acatccacct tgttttcttc
     4861 attgttctta tccacaatgc attaggtttt actgttggat actggtttcc gaaacttttc
     4921 aaagaaaatt cgcaaaacag ccgtaccatt gccattgaaa ccggtattca gaattccggt
     4981 ttgggtttgg cgctgatttt caatccgaaa attttccctc ccgaactcga actgggcggt
     5041 atggctttta tcgcagcctg gtggggcgta tggcacattg tggccggctc aggattggct
     5101 ttttactggg gaagacggcc atctaaatcc gccaaaaggg actttgccga gttttgagtt
     5161 cattagttta tagtaaaata gctaaaatct gtgctcgata aataacaata ttttaccccc
     5221 ccggactttt tcccaagggg taatttgctt tgtttccgtg ggcgagattt aatttacata
     5281 ccgacgtttt ttttacagcg ttttgcatgt tttattaatg caggacaaat atattattta
     5341 tcaaaataca gtttgaaaat atcaacacat tccaaaaatt acataaattt tttcaaaata
     5401 ttttacgcat gaaagtaaaa cacaataaat caagccaaaa attccccttt ggggcttggg
     5461 gggctgggta tttcctcatg cagcttcatg taaatcgtgt gtttcgcttg cattttcata
     5521 aattagtttt tacaggtaaa gaaaatattc ctcaggacaa acctctcatc tatgctccta
     5581 cacaccgaaa tgcgttgatg gatgcactga ttttggtgca tgaaaacgtg aaacaacaag
     5641 tggtttttct ggcacgtgcc gatattttta aaaaaccgct ggtgattaaa atcctgtatt
     5701 tatttaaaat tatgcccgtg tatcgcattc gcgacggcaa ggaaaatctg ggtaaaaaca
     5761 aggagatttt cgtgcaatgt ggcgaaattt tgaaaaacgg aaatcctctt tgcctttttc
     5821 ctgaagccag attcaatccg catcagaacc tgctcccgct gcaaaaagca attccgcgca
     5881 tcgcactgcc cacggaggcc gaaacgggct ttaccctcga tacacacatc gttcccgtgg
     5941 cgttttatta cacggcgaaa gatcgttttt tgtcggattt gtacgtaact tacggcaaac
     6001 ctgttcgggt agctgattac aaagaacttt acttaacaga tgaaaatcag gcgattaaca
     6061 agcttcgcaa tgatttggat gaccgtctgc gtcaatatgt agtggatatt ccggaggagg
     6121 attacgggga aatttcggcg ttgatagacg tgaatgccaa cgatttagcc
//
LOCUS       DAORKP010000126         6048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2617_length_6048_cov_8.218755, whole genome shotgun sequence.
ACCESSION   DAORKP010000126 DAORKP010000000
VERSION     DAORKP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2617_length_6048_cov_8.218755"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..903)
                     /gene="yaaA"
                     /locus_tag="PLN75_07400"
     CDS             complement(130..903)
                     /gene="yaaA"
                     /locus_tag="PLN75_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRG03979.1"
                     /translation="MLTIISPAKIQNFNPQNTISDYTLPVFLKEANMLAKQLGSYSIS
                     ELASLLHINSKIAEENANRYFHWKTPFTPENSKQAIFTYNGEVYRGLDAATLNNEEIL
                     YLQNHLRILSGLYGALKPLDLIQPYRLDVKDRLTTEKGDNLYSFWKKRVTDETAKALK
                     KSDNPNVLLNLASAEYTRMLDIKQLKVRIIDFDFLQYNPDSDTFKPITIYLKRARGLM
                     VRFVAQNRITDPEDLKAFSADGYWYSERFSTENKMVFVQ"
     gene            complement(1030..2232)
                     /locus_tag="PLN75_07405"
     CDS             complement(1030..2232)
                     /locus_tag="PLN75_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRG03980.1"
                     /translation="MSEFSTHQSQKIADLTELFEAIIYKQKAAETIHKFQSTTIANIT
                     PDDVVIFVHQLVDSGYPMDDIKSGINKLLNVLRRPLDDFRYEKPADDSFFGVCIQNNA
                     QAISHLNKIRPYILEINKKALTSEIKTELITLWTDVQKFTDYYQIKENILFPLVEKYV
                     ADYRCISVMWSIHDDIRKNINQLIDILNLNDTDLKHLNRLTGDIFYNIYSIIFREERL
                     LFPVIQKYIPADEIENIWNESVESGFPYINPPKKENVMEKNKVPSDLIELKSGQLTAE
                     QIVLVFSHLPVDITFVDENNKVRFFSTPKERIFHRTNAVIGRDVHNCHPKESVHVVEQ
                     IVEAFRAGKKDKADFWISMRNGKRVLIQYFAVRNEHNEYKGVIEVTQEISEIQRLEGD
                     RRILDWEG"
     gene            complement(2305..3120)
                     /gene="pyrF"
                     /locus_tag="PLN75_07410"
     CDS             complement(2305..3120)
                     /gene="pyrF"
                     /locus_tag="PLN75_07410"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRG03981.1"
                     /translation="MNKQQLVENIFHKKTFLCVGLDTDVDKIPEHLFDVTDDPVFEFN
                     KQIIDATADLCVAYKLNLAFYESIGLEGWDALERTVDYIRSSYPDQFIIADAKRGDIG
                     NTSAMYARTFFGNMDFDAVTVAPYMGHDSVSPFLTYENKWTILLALTSNKGAFDFQYM
                     EENGEKLFEKVLKTSKEWGSDENMMYVVGATKAEMLTEIRKIIPNHFLLVPGVGAQGG
                     SLQEVVKYGKNDECGLLVNSSRQIIYASSDVDFAEKAREEALKVQQEMAELLK"
     gene            complement(3144..4223)
                     /gene="prfA"
                     /locus_tag="PLN75_07415"
     CDS             complement(3144..4223)
                     /gene="prfA"
                     /locus_tag="PLN75_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRG03982.1"
                     /translation="MSSLIEKLDGLQHKFEEISMLITDPAVIADMQRYVKLNKEYSEL
                     SKIMNAQKEYSTTLSNIQEAKDILSNESDTELREMARMELEMLESKVPELEEKIKFLL
                     IPVDPEDAKDVVMEIRGGTGGDEAAIFAGDLFKMYTRFCESKGWKVDVTDFSEGTIGG
                     YKEIIFTVTGENVYGTLKYESGVHRVQRVPETETQGRVHTSAATVAVLPEADEFDVQI
                     NPADIDFHTSRSGGAGGQNVNKVETKVQLTHRPTGIVVVCQQARTQLGNREIAMEMLR
                     TKIYNMEYEKHLSAIASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLAAFM
                     NGDIQDVINQLTVVENAERMKESEL"
     gene            complement(4319..5263)
                     /locus_tag="PLN75_07420"
     CDS             complement(4319..5263)
                     /locus_tag="PLN75_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRG03983.1"
                     /translation="MKKESLKKFAYLSIAAAVVTIGLKFFAYLYTGSMGLLSDALESC
                     VNLVAAVVALIMIHISEKPADEGHEFGHTKAEYFSSAIEGSLILVAAFSIIWSAVPRL
                     IAPQPIENVGLGLLISTSASLVNLGVSLVLISNGKKRKSLLLEADGKHLMTDVWTSAG
                     VIAGVLIVKLTGFLILDPVIAVLVALNIIWTGFTLIKRSANGLMDASITEEELKKVKH
                     YLDGFKTDTIEYHSLMTRQAGQRKFISLHLLVPGEWSVQQGHDFAEKIEESIEDMFSE
                     PVTVSSHIEPIEDPASMRDIGIDRKKITENNQFTITKS"
     gene            complement(5323..>6048)
                     /locus_tag="PLN75_07425"
     CDS             complement(5323..>6048)
                     /locus_tag="PLN75_07425"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRG03984.1"
                     /translation="TNGEREMAYALYLAGFDVKDVHMTDLASGRETLEDLNLIVFCGG
                     FSNSDVLGSAKGWAGGFLYNEKARKALENFYKRTDTLSLGICNGCQLMVELNLINPEH
                     EKRAKMLHNDSHKFESGFVTLNIPENDSVMFNTISGSKLGIWVAHGEGKFSLPMGENA
                     YKIVAKYNYDGYPANPNGSDYAVAGICSADGRHLAMMPHLERAIFPWQCAHYPADRIK
                     NDEITPWIEAFVNARKWVETNKK"
BASE COUNT         1543 a         1252 c         1277 g         1976 t
ORIGIN      
        1 gatataaaat tagtttcttt gtgtaacttg gtgttttagt gtctttgtgg caaaatcaag
       61 gttttcggag cagactcatt gttttaaaaa aaagtcgaga cgggttctat ttaataaatc
      121 gtgcaagctt tactgcacaa ataccatttt gttttcggtg gaaaatcgct cgctgtacca
      181 gtatccatcg gcagaaaaag ctttcagatc ttcgggatcg gtaatccggt tttgagctac
      241 gaaacgcacc atcaggcccc gtgctctttt caggtagatg gtgattggtt tgaatgtatc
      301 ggaatcggga ttatactgca gaaaatcaaa atcaatgata cgcactttca gctgttttat
      361 atccagcatt cgggtatatt ctgccgacgc cagatttaat aaaacgttcg gattatctga
      421 ttttttcagt gctttcgccg tttcgtcagt cacccgtttt ttccagaatg aatacaaatt
      481 atcacctttt tcggtcgtga gtctgtcttt tacgtccaga cggtagggct gaattaaatc
      541 aagcggtttc agtgctccgt acaggccaga gagtattcgc aggtggtttt gcaggtaaag
      601 aatttcttca ttgttaaggg tagcggcatc caacccacga tatacttcgc cgttgtacgt
      661 aaaaatggcc tgttttgaat tttccggtgt gaaaggtgtt ttccagtgaa agtagcgatt
      721 tgcattttct tcggcaattt ttgaattgat atgcaataaa gacgccagtt cgctgatgga
      781 gtaggaacca agctgttttg ccagcatgtt tgcttctttc agaaaaacgg gcagagtata
      841 atcagaaatc gtattttgag gattaaaatt ttgtatttta gcgggtgaaa tgatggtaag
      901 cattgtctga actttgattt ttttgattta tatgataaaa acagacaaaa tagctttttg
      961 agtcaaagaa actatgatat agaatatgtg gaatatatca ttttaatcct aaaaatcaag
     1021 tttcagacgt tatccttccc agtccagaat tcttctgtca ccttccaggc gctgaatttc
     1081 gctgatttcc tgcgttactt ctattactcc tttgtattca ttatgttcgt ttcgaacagc
     1141 aaagtactga atcagcaccc gttttccgtt gcgcatgctt atccagaaat cggctttgtc
     1201 ttttttgccg gctctgaatg cttccactat ttgctctact acatgaacac tttcttttgg
     1261 gtggcagttg tgtacatcgc ggcctataac agcattcgta cgatgaaaaa tgcgctcttt
     1321 cggcgttgaa aagaaacgaa ctttgttatt ttcatccaca aacgtaatat ctaccggcaa
     1381 atggctgaaa accaatacaa tctgttcggc ggtgagttgt ccgcttttca gttcgattaa
     1441 atctgaaggg actttatttt tttccattac gttttctttt tttggtggat tgatgtaagg
     1501 aaatcccgat tcaacacttt cgttccagat gttttcaatt tcatctgccg gaatatattt
     1561 ctgaataacc ggaaaaagca accgctcttc tctgaaaata atcgaataaa tgttgtaaaa
     1621 tatatctccg gtaagtctgt ttaaatgctt taaatcagta tcgttaagat tcagtatatc
     1681 aattaattga tttatgtttt ttctgatgtc atcgtgtatg gaccacatca cggagatgca
     1741 tcggtaatcg gcaacgtatt tttcaacgag cggaaaaaga atgttttcct tgatctgata
     1801 atagtctgta aatttctgta catcagtcca aagcgtaatg agctcagttt tgatttcact
     1861 ggtcagtgct tttttgttga tttcaagaat gtagggccgg attttattca ggtggctgat
     1921 agcttgtgca ttgttttgaa tgcatacgcc gaaaaaactg tcatctgcag gcttttcata
     1981 ccgaaaatcg tccagtgggc ggcgaagcac attgagcaat ttgttgatac ccgatttaat
     2041 gtcatccatc gggtaaccgg aatctacgag ttgatgtaca aatattacca catcatcggg
     2101 tgttatgttt gcaattgtgg ttgactgaaa cttgtggatg gtttcagcag ctttttgctt
     2161 gtagattatc gcttcgaaaa gttcggtcag atcggcgatt ttttgggatt gatgcgttga
     2221 aaattcagac atattcgaaa agttagatgt ttgaagttag aaattagaaa agttgggagc
     2281 taactcagaa ctcagaactc ttatttactt gagcaattcc gccatttcct gttgcacctt
     2341 cagcgcttct tctcttgctt tttctgcaaa atcaacgtcg gatgaagcat aaataatctg
     2401 acgggaggaa tttaccaaca atccgcattc gtcgtttttt ccgtatttaa ctacttcctg
     2461 aaggcttcca ccctgagcac ctacaccggg tacgagcaga aagtgatttg ggattatttt
     2521 acgaatttca gttagcattt cggctttcgt ggcaccaacc acatacatca tattctcatc
     2581 cgaaccccat tctttagagg ttttcagtac tttttcgaat agtttttcgc cgttttcttc
     2641 catgtactga aaatcaaaag ctcctttatt ggatgtcaga gccagcagaa ttgtccattt
     2701 attttcatag gtcagaaacg gagaaacgct gtcgtgtccc atatacggtg caaccgtgac
     2761 ggcatcaaaa tccatatttc cgaaaaacgt gcgtgcatac atggctgaag tattaccgat
     2821 atcgccccgt ttggcatcgg caatgataaa ctggtcggga taactggagc ggatgtaatc
     2881 caccgttcgt tccaatgcgt cccagccttc cagtccgata ctttcataaa atgccagatt
     2941 gagtttgtaa gccacacaga gatcggcagt tgcgtcaatt atttgcttat tgaactcaaa
     3001 aaccgggtcg tccgttacat cgaaaagatg ttcgggaatt ttatcgacat cggtatccag
     3061 accaacgcaa agaaatgttt ttttgtgaaa aatattttca acaagttgtt gtttgttcat
     3121 agcgtaaatc attaaaaggg tttttataat tcactttctt tcattcgttc tgcattttcc
     3181 accaccgtga gttgattgat tacatcctgt atgtcgccgt tcataaatgc ggccaggttg
     3241 tagatggtgt aattgattct gtggtcggta attcgtccct gtggatagtt gtacgtgcgt
     3301 atttttgccg atctgtcgcc ggtagatacc atggttttac gtttcgaagc aatcgctgaa
     3361 aggtgttttt cgtattccat attgtatatt ttcgtacgaa gcatttccat ggctatttca
     3421 cggttaccga gctgagtacg agcctgctgg cataccacca caataccggt tggacggtga
     3481 gtaagctgca ctttggtttc cactttgttc acattctgac cgcctgcacc gcctgagcgt
     3541 gacgtatgga aatcgatatc ggccggatta atctgtacat caaattcgtc ggcttcgggg
     3601 agcacagcca cagtggctgc cgaggtgtgt acacgaccct gggtttcggt ttcgggtacg
     3661 cgctgcacgc ggtgaacccc gctttcgtat ttcagcgtac catacacgtt ttcgcctgtc
     3721 acggtgaaga taatttcctt gtatccgcca atggtgcctt cactgaagtc agttacatca
     3781 actttccagc ctttgctttc acagaaacgg gtgtacattt tgaaaagatc tcctgcaaaa
     3841 atggctgctt catcgccgcc tgttccgcca cgtatttcca tcactacgtc tttggcatct
     3901 tccggatcaa ccggaatcag caggaatttg atcttttctt ccagctcggg aacctttgat
     3961 tccagcattt caagctccat tcgagccatt tcgcgcagtt cggtgtcgct ttcgtttgaa
     4021 agtatgtctt tggcttcctg tatgttggac agcgtggtgg agtattcttt ctgagcgttc
     4081 atgattttcg atagctcact gtattctttg ttgagtttca cgtagcgctg catgtcggct
     4141 atcacagccg gatcggtgat gagcatggat atttcttcaa atttatgttg aaggccgtcc
     4201 agtttttcga tgagtgatga catatatttt atttacgatt tacaatttta gatttacgat
     4261 tgggtttatt ttctatattt taatatattg ttatacagca atttgcaatt atttattttt
     4321 acgattttgt tatagtgaac tgattgtttt cagtgatttt ttttctgtca attccgatat
     4381 cgcgcatcga agccggatct tctatcggtt caatgtgaga tgaaacggtt acaggttcgg
     4441 aaaacatgtc ttcgatactt tcttctattt tttctgcaaa gtcgtgtcct tgctgtacgc
     4501 tccattcacc cggaacgagc agatgaagcg aaataaattt tcgttgtccg gcctgacgtg
     4561 tcatcaacga atgatattca attgtatctg ttttgaaccc gtcaagataa tgttttactt
     4621 ttttcagttc ttcttcggtg atggatgcat ccatcagtcc gtttgctgag cgtttgatta
     4681 atgtaaatcc ggtccagata atattcaaag ccaccaaaac ggcaataaca gggtcaagaa
     4741 tcaaaaaacc ggtcaatttc actatcagta ctccggctat gactcctgcc gaagtccata
     4801 catcggtcat caggtgtttg ccgtctgctt cgagtagcaa cgattttcgt tttttcccgt
     4861 tactgattaa aaccagggaa actcccagat tgacaagcga agcgctcgta gatattaaca
     4921 aacccaagcc tacgttttca attggctgag gtgcaataag acgtggaaca gccgaccaga
     4981 tgatgctgaa tgcagctacc agaatgagac ttccttcaat ggcactggag aaatactccg
     5041 ctttggtgtg cccgaattca tgtccttcat cagccggttt ttcagaaatg tgtatcatta
     5101 tgagcgctac tacggcagct accaggttta cacacgattc gagcgcatcg gaaagcaaac
     5161 ccatggaccc tgtgtataag taggcaaaga atttaagccc gatggttacc actgcagctg
     5221 caatggaaag gtaggcaaat ttttttaatg attctttttt cattagatga agtgagaaat
     5281 tttgtaatga ttacactctg atctgctgtg atttgaattt cattattttt tgttagtttc
     5341 cacccatttt cgtgcattca caaaagcttc gatccagggc gtaatttcat cattcttaat
     5401 gcggtcggca gggtagtggg cgcattgcca tgggaaaatg gctcgttcca gatgcggcat
     5461 catggccaga tgacgtccat cagcagaaca gattccggca acagcataat ctgaaccgtt
     5521 cggattggca ggatatccat cataattgta ttttgctaca attttgtacg cgttttcacc
     5581 catcggaagt gagaatttac cttcgccgtg tgccacccaa atgccgagtt tacttcccga
     5641 aattgtgtta aacatcaccg aatcgttttc gggaatattg agcgtaacaa atccactttc
     5701 aaatttatgg ctgtcgttgt gaagcatttt tgcacgtttt tcgtgttcgg gattgatcag
     5761 gttcagttct accattagct ggcagccgtt gcaaatgccc aaacttagtg tgtctgtgcg
     5821 tttgtaaaag ttttcgagtg ctttccgggc tttttcgttg taaagaaaac cacctgccca
     5881 acctttggcc gatccaagca catcggaatt ggaaaaaccg ccgcagaata ctatcagatt
     5941 caggtcttca agtgtttcgc gtcctgaagc caggtcggtc atgtgcacat ctttcacatc
     6001 aaaaccggcc agataaagcg cataagccat ttcacgttcg ccgtttgt
//
LOCUS       DAORKP010000127         6043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2620_length_6043_cov_7.076319, whole genome shotgun sequence.
ACCESSION   DAORKP010000127 DAORKP010000000
VERSION     DAORKP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_6043_cov_7.076319"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1259..2839)
                     /locus_tag="PLN75_07430"
     CDS             complement(1259..2839)
                     /locus_tag="PLN75_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRG03985.1"
                     /translation="MNSELIVDVRPSQISIALLEDKRLVELNEEGREENFAVGNIYLG
                     RVKKIMPGLNAAFVDVGYEKDAFLHYLDLGNNFNTLNGFIKQVQSDRKKTPLLTKAKQ
                     LPEIEKNGSINDILKTGQEIVVQISKEPISTKGPRLTAELSIAGRFLVLIPFADKVSV
                     SQKIKTEEERIRLKQLIQSIKPKGFGVIVRTVAEEKKVAELDNELKILVKRWEETIPR
                     IQKSQGVSLISEELGRTVGIIRDLFNSNFKNIYVNDKDVHTEVRDYVELIAPERKDIV
                     KLYKEDIPIYDHFGITKQIQSALGKTVSFRSGAYLIIEHTEAMHVIDVNSGNRSKAGS
                     GQEINALEVNLAAADEIARQLRLRDMGGIIVVDFIDMHEGENRQKIFDRMRENLTNDR
                     AKHNILPLSKFGLMQITRQRVRPVIDIDIEETCPTCYGSGRTKPSILFTDQLESKIDY
                     LVSKLKVKDFTLHIHPYVDAYISRGLPSMKMKWKLKFGIGCKVIPSQKLGFLQYKFYD
                     KKGEEIDLQEEIEKISSK"
     gene            complement(3148..3420)
                     /locus_tag="PLN75_07435"
     CDS             complement(3148..3420)
                     /locus_tag="PLN75_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG03986.1"
                     /translation="MTKADVVNEIAKNTGIDKATVLVTVENFMDVVKASLARKENVYL
                     RGFGSFIIKERAQKTARNISKNTTIIIPAHSIPAFKPAKTFSGTVK"
     gene            complement(3552..4667)
                     /locus_tag="PLN75_07440"
     CDS             complement(3552..4667)
                     /locus_tag="PLN75_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRG03987.1"
                     /translation="MNKKLFFIPLAILMLSACQQSGYKISGTIENPGFFDSTMVVLSE
                     RINREWVDLDSTVVLNGSFAFEGKSDSAKVCYLRFKTISGDRKTGDFILENGKMTASM
                     DSSFNVVVKGTPQNDVLAQFYSAENQIQKKANEKMKEIMPENTQEPTPEMMAAFREFS
                     KEINKELKSNLITNVMNHVNTLAGSHIFMSNFYGMTTEEKDALFAKMDVKTKAIPRIA
                     ELMAATEVEKKTAVGQPYVNFTLTTPDEKEVSLSDFIGKTDYLLIDFWASWCGPCIRS
                     FPELTAFYAKNKGSKFEILGVSLDKEKPDWTNAILKYGLTWKHVSDLKYWDSEAARLY
                     AVNSIPATVLIDKNGKIVGRNLELDEMQNLLNQQVTK"
     gene            4840..5901
                     /gene="mutY"
                     /locus_tag="PLN75_07445"
     CDS             4840..5901
                     /gene="mutY"
                     /locus_tag="PLN75_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRG03988.1"
                     /translation="MNSKQTAAIRKILNEWYLINKRTLPWRETKDPYKIWISEIILQQ
                     TRVNQGLDYYNRFIERFPDVKSLAEAGEDEVLKYWQGLGYYSRARNLHKAAKQIMNKY
                     NGAFPTVYHEVLSLSGVGEYTAAAVCSLAYHQPYAVVDGNVYRVLSRLTGSSTPIDTS
                     AGKKEFSAVAQQLLSITSPDVHNQAMMDFGALQCTPANPDCEICVLQDFCEAYKLNIV
                     GQLPVKQGKTNVKNRYFNYFFIKNDDYTYLKKRTGKDIWQNLYEFPLIETDKEMNFQE
                     LTETESFRQLFGETELTVQKISHPVKHILSHRIINAVFYKINISHENETIKDFEKLLL
                     SELNQFPVSRLTELYLEKG"
BASE COUNT         1707 a         1150 c         1159 g         2027 t
ORIGIN      
        1 agggaattat caggaaatca acatatttat ttttatgaaa aagttatttg atcacttttg
       61 caacgaactc atcctcactt tctgattttg ttaagattta ttcagattat cgatttttca
      121 ttacttttta attcgcctct tcaccattct ccaaagggga aaatcattgg gtcggttgaa
      181 attagtacat tcattttatt tctaaaaaaa gttccggacg aattcaatat ctgacttttt
      241 aaaagaacgt ttttataaaa tcttaatcat atttttactt cccaaaattt taaatgaact
      301 cacatgaatg cactcaaaat cagctattcg gtcggcgctg gtcccggtaa caaagaggta
      361 ttcaaattga tcggccggaa ttctgctgat taaatgaaac agaaaagcca gaaaaaatac
      421 tgaaaaaaaa ctaatcgtat tgttttcata acttagtata aacaaaagat taaccagtat
      481 taaatctatg agaaattgtg tagtgaaaat atgagagttc ttatgaaaac ataagaaaag
      541 ttcatcacag taaaactgtg gttcatgggt gataaaattg gtaaaaagaa tagaaaatat
      601 tgaatgtggg gacggaagta ctactttgta ttacagaagt tgccgtactg agaaaattgg
      661 tggcgtcata caatttttct ataaattgtt aaagtacatt tttaatttaa acaattcagt
      721 gctgtgatag tttgttaaat ttgttgagtt gcgctgtaaa tcaaaaagat tctaaaaaaa
      781 atgtttcaaa ttgaggcggt gtaaagtcat cggtcacttt tcccttcgtt ctttcggata
      841 taaaattcca ataaaagtat atttaattct attagctgtg gctggatgaa atgccggttc
      901 ggctgcaatg gcaagcgaac agcatcggga tgaaagtagc ttgaaaattc aatatcattg
      961 tttcaactgc ctgaatctga ctctttatta tttttgcaaa ataccggttt atctgtagaa
     1021 gtttgttttt tgaaacttca aaaaaggtaa agtggcaaaa atatatttga aagtaaaaaa
     1081 aattagcagt actgaaagga ttaaattttg aacttttccg gagttccgga tattaattaa
     1141 gctgattgta acttattaat caggaatttg tgacagaaga tatgcaggta atgatgagtg
     1201 cagggacttt atataaatca aaaaagagct gcccgtatga gcagctcttt tttgattttt
     1261 acttagacga aatcttttct atttcttcct gaaggtcaat ttcttctcct tttttgtcgt
     1321 aaaatttgta ttgcagaaaa cccagtttct gggacggaat gactttgcag ccaattccga
     1381 attttaattt ccatttcatt ttcatggatg gcaatcctct tgaaatgtag gcatcgacat
     1441 acgggtggat atgcagcgtg aagtctttca ctttcagttt gctgaccaga taatcgattt
     1501 tgctttccaa ctgatcggtg aagagtatgg acggtttggt gcgtccgctt ccgtaacatg
     1561 tcggacaggt ttcctcgata tcaatgtcaa taaccggacg aacccgttgt cgggtaattt
     1621 gcatcaggcc aaacttgctg aggggcagaa tgttgtgttt tgccctgtcg ttggtcagat
     1681 tttcacgcat ccggtcgaaa attttctgcc ggttctcacc ttcgtgcata tcaatgaagt
     1741 ccaccacaat gatgcctccc atatcccgca aacgcaattg ccgggctatt tcatcggctg
     1801 cagccaaatt tacttccagt gcattgattt cctgtccgct tccggctttt gaccggtttc
     1861 cgctgttgac gtcaatcaca tgcatggctt ctgtgtgttc gataatcaga taagctccgc
     1921 ttctgaacga cactgtctta ccgagtgcag actgtatctg cttggtaatc ccgaaatgat
     1981 cgtaaatcgg gatgtcttcc ttgtaaagtt ttacaatgtc tttccgttcg ggagctatca
     2041 gttctacgta atcgcgaact tcggtatgca catccttgtc gttgacgtag atgtttttga
     2101 agttcgaatt gaataaatct ctgattattc ctaccgtgcg acctaattct tctgatataa
     2161 gcgatactcc ctgcgacttt tgaattctgg gaatggtttc ttcccaccgt ttcaccagta
     2221 ttttcagttc attgtcgagt tccgctactt ttttttcttc ggcaaccgta cgaactatta
     2281 cgccaaaccc ttttggcttt atactctgta tcagttgttt cagtctgatt cgctcttctt
     2341 ccgtttttat tttctgcgac acagaaacct tgtcggcgaa tggtatcagt accaggaatc
     2401 ttcctgctat ggagagttcg gctgtgagtc gcggaccttt ggtggagatg ggttcttttg
     2461 agatctgaac cactatttcc tgtccggttt tcagtatatc gtttattgag ccgtttttct
     2521 caatttcagg aagttgtttg gctttagtca gtagaggtgt tttcttacgg tcgctttgca
     2581 cctgtttgat aaagccgttg agagtgttga aattgttacc taaatcaaga tagtgaagaa
     2641 aagcgtcttt ctcgtagcca acatctacaa acgctgcatt aagtccgggc attatttttt
     2701 tcacccttcc gaggtagatg ttcccaacgg caaagttttc ttcgcgtcct tcttcgttga
     2761 gttctaccaa ccgtttgtcc tcaagcagcg caattgaaat ctgagacggt cttacatcta
     2821 caattaactc gctgttcact tgttttggat ttatgtattt ttgttttcaa cctgtcggag
     2881 aacgggttat cactcttttt ttcgttaaaa aaagcgtggt tttttgctgc tgtccggcag
     2941 gtctgtgttt ataaaaaaca aacttaaatc cattgaattc ggttttaagt ttgtttcttt
     3001 ggacctttta agaactaatg tccaacagac tatttctttt tatgtctatt ttttctcagt
     3061 ctttttttcc gtttgtgagt agacatttta tgtctttttc tttttttacc gcttggcatc
     3121 ttttgttaaa atttaatcgt tattgaatta ttttacagta ccggaaaaag tttttgccgg
     3181 tttgaatgca ggaatgctgt gcgccggaat gataatagta gtgtttttag aaatgttgcg
     3241 agcagttttt tgagctcttt ccttgatgat aaaactaccg aaacctctca gatatacgtt
     3301 ttcttttctt gcaagagaag cttttacaac atccataaaa ttctctacag ttactaatac
     3361 agttgcttta tcaatgcctg tatttttagc aatttcgttt acaacgtctg ctttagtcat
     3421 ttttttttaa ttttttataa gttaaataat atagtgtatt tatttttttt cgaaacggac
     3481 tgcaaatata gagtttttct tctgattaaa aaagaattag ttacgttttt ttcaaatttt
     3541 aaatggaaag tttatttcgt aacttgttga ttgagtaaat tttgcatttc gtctaattcc
     3601 agatttcttc caacaatttt tccgttttta tctatcagca cagtagcagg aatggagtta
     3661 acagcataga gccgggctgc ttcactgtcc cagtatttga gatcagacac gtgtttccat
     3721 gtcagtccgt atttcaatat ggcattggtc cagtccggtt tttctttatc aagcgataca
     3781 cccagaattt cgaattttga ccctttgttt ttggcataga aagctgtcag ttcggggaat
     3841 gaacggatgc acggaccgca ccacgacgcc cagaaatcaa tgagcaggta gtcggttttg
     3901 ccaataaaat ctgagagcga aacctctttt tcgtcgggag tagtgagtgt gaagttaacg
     3961 tacggttgac ctactgccgt tttcttttcc acttctgtag cagccatgag ttccgcaatg
     4021 cgtggaatgg ctttggtttt tacgtccatt ttggcaaaaa gtgcgtcctt ttcttcggtg
     4081 gtcattccgt agaaattact cataaagatg tgcgagccgg ccagtgtatt cacgtgattc
     4141 atcacattgg ttatcagatt ggatttcagt tctttattta tttctttgga aaattcgcgg
     4201 aatgcggcca tcatttcggg agtaggctcc tgcgtatttt caggcattat ttctttcatt
     4261 ttctcatttg cttttttctg gatttgattt tcggcagagt aaaattgagc cagcacatca
     4321 ttttgaggcg ttccttttac caccacgttg aaactgctgt ccattgaggc ggtcattttt
     4381 ccgttttcga ggataaaatc gccggttttc ctgtctcctg aaatggtttt gaagcggaga
     4441 tagcacactt tggctgaatc tgatttgcct tcaaatgcaa aacttccatt caaaaccacg
     4501 gtgctgtcca aatccaccca ctcccgatta atccgttcac tcagcacaac cattgtgctg
     4561 tcaaaaaatc cgggattttc aatagttccg gaaattttat acccgctttg ctgacatgct
     4621 gacaacatta atattgccaa tggaatgaaa aataattttt tgttcataat attctagcct
     4681 atttaatttt ttgattgtaa tttcgcctta ttctttttac gtcggaaaat tgtacaaact
     4741 gcatgcaaag ttgcaacttt ttctgcaacc tgacaaaatt tgcgacaaca ttttttcgaa
     4801 ttaagtaatt aattttcaaa taaataaaat ccaacacttg tgaacagcaa acaaactgcg
     4861 gctatccgga aaatactgaa cgaatggtat ctgatcaaca aaagaacgtt accatggagg
     4921 gaaactaaag acccttataa aatctggatt tcagagatta ttcttcagca aacacgtgta
     4981 aatcagggtt tggactatta caatcgtttt atcgaacgtt ttccggacgt gaaaagttta
     5041 gcagaagccg gtgaagatga agtgctgaaa tactggcagg gactgggtta ttactcccgt
     5101 gcccgaaacc tgcacaaagc tgcaaaacaa atcatgaaca agtataacgg cgcatttcca
     5161 actgtttatc acgaagtgtt gagtttatcg ggcgtgggtg aatacacagc agccgccgtt
     5221 tgttcgctgg cttaccacca accttatgcc gtagtggacg gaaatgtgta tcgcgtactt
     5281 tcgcggctta ccggatcatc cactcccatc gacacatcgg cgggcaaaaa ggaattctcc
     5341 gctgttgcac agcagctgct ctccatcacc agccccgacg tacacaatca ggccatgatg
     5401 gattttggag ccctgcaatg cacacctgca aaccccgatt gtgaaatctg tgtgcttcaa
     5461 gatttttgcg aagcatataa actgaatata gttggtcaac ttcctgtgaa acaaggtaaa
     5521 acaaacgtga aaaaccgcta cttcaattat tttttcatta aaaacgacga ttacacctac
     5581 ctgaaaaaaa gaaccggaaa agatatctgg caaaatttgt atgaatttcc attgattgaa
     5641 actgacaaag agatgaattt tcaagaactt accgagacag aaagtttcag gcagcttttt
     5701 ggtgaaacag aactcacggt tcaaaaaata agccatcctg taaagcacat tttgagccac
     5761 cggattatta atgccgtttt ttataaaata aatattagcc atgaaaacga aactatcaaa
     5821 gattttgaaa aattactact aagtgaatta aatcaatttc cagtttcaag actcaccgaa
     5881 ttatacctgg aaaagggatg aagtttgata taattgatga taatctgaag taacacttcg
     5941 aaattacata aataaacttt cgcagattat aacttactat ttgcagataa gattgctaac
     6001 gggcgacctc gtccggtgag ccgaaaaatc agagagagcg gcg
//
LOCUS       DAORKP010000128         5960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2683_length_5960_cov_8.619136, whole genome shotgun sequence.
ACCESSION   DAORKP010000128 DAORKP010000000
VERSION     DAORKP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_5960_cov_8.619136"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLN75_07450"
     CDS             complement(<1..242)
                     /locus_tag="PLN75_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG03989.1"
                     /translation="MNYSDRYVKLNTCFTPEKDTHLPDAKIDVKNFCFESNSFDAGHK
                     ETSIHRNEIIVAENLKFRYPVFVPRGRFRADKAILL"
     gene            450..1307
                     /locus_tag="PLN75_07455"
     CDS             450..1307
                     /locus_tag="PLN75_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRG03990.1"
                     /translation="MNSKIPPYLYEKGNNVRLILFTAFFALLFINIFQPFGSRGWYPK
                     VSDLKYFVFSSLIILTGMLVVVVSRMIMHRYTHRHNISYVQFAIWILCEILAMSLFYS
                     LFTKFIPSQGAERQFSEIFRSCTINTGLILLLPYSISWLYFSWKEKNRMIEKLKAEES
                     APEVHTRPMVAFMDEKGELKISLMVENLLYIDAADNYATIYYLNKSKLSHYLLRNTLK
                     WMDENLTNDTPLVRCHRSYMVNLDKVKVLRKTKTGIYIELDAENTPDIPVSKTYYERF
                     MKKFSQYSV"
     gene            1488..1901
                     /locus_tag="PLN75_07460"
     CDS             1488..1901
                     /locus_tag="PLN75_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRG03991.1"
                     /translation="MKLSKTSEYALRILIYMAQKPEELYTAKFLVEKLNVSDKYLRRL
                     MTDLSKSGFIKSTQGRDGGYTFAKEIHEISLYEVIDSVEGMNKLNDCVLGFDECSCTN
                     PCAMHNEWVVVREKLNQLFTETRLSDLDFERISRY"
     gene            2137..2499
                     /locus_tag="PLN75_07465"
     CDS             2137..2499
                     /locus_tag="PLN75_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRG03992.1"
                     /translation="MKTINYKDLVTAQNPHGVDARKLHSNDDVQVVHMHLKPGQSLKK
                     HITAVNVFFYVLEGEGVVEIGDEKQTVTKDCLIDSPKDIPHCLYNESTTDFRVLVVKT
                     PSPTAAQNTKAIENMLKQ"
     gene            2509..3054
                     /locus_tag="PLN75_07470"
     CDS             2509..3054
                     /locus_tag="PLN75_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRG03993.1"
                     /translation="MNAIKILVDEHDIILRMIDVTRSMLVDTSTLNTDHVEQVVDFIK
                     NFADKYHHLKEEDVLFVEMENHGMSRQSGPVGVMLHEHEQGRAYVKQAEEGILRLKSG
                     DIHAAGAIKESLLNYSTLLTNHINKENNILYPMAERLLPQTVMLAMSENFGEAITIAS
                     NNEFKQKYLKMADELSRIYMK"
     gene            3082..3777
                     /gene="ric"
                     /locus_tag="PLN75_07475"
     CDS             3082..3777
                     /gene="ric"
                     /locus_tag="PLN75_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRG03994.1"
                     /translation="MKTNKQTKIGDIVTLDFRAAEIFKNAGIDFCCGGSQTLGDACRD
                     KNLDVAAIETQLEQLENSAPDSSHMFNEWKLDFLCDYIVNTHHQTVMNLLPQLTFYTQ
                     KIAEVHGNNHPELLEIAGLFAQVDTELRQHLRNEEEVLFPAIKEVMKTNSDESKATII
                     SEITRMTGEHEFAGGAMDKINELSRRYAVPEDGCNTYRVAYKLLEQFEDDLHIHVHLE
                     NNILYPKAMKLTV"
     gene            3934..5169
                     /locus_tag="PLN75_07480"
     CDS             3934..5169
                     /locus_tag="PLN75_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRG03995.1"
                     /translation="MSELINNSQNRKDKLKALILKLHEGETEESVRNELLLTLSQIPY
                     GEVVEVEQELIAEGLPEEEILDLCDAHSSVLEGRVDLSALKPVPDGHPVHVFRRENEE
                     IRKTTNAIFARLSDIIHGSEENLHEKVLNLRGLFNSLYDVDKHYQRKEYLVFPYLEKL
                     EITGPPKVMWGKHDEIRELIKGSIEVLQVDSLSREELEAAADMVLKPAARGAQEMITK
                     EEEILFPMALDALTEAQWYEISRQSIEIGFCLYDPEVDWRPEWVEEQTVNQLQKSGSH
                     IQLPSGSFSAEEIMAILNTLPVDMTFVDKDDKVKYFSQGAERIFQRNRAILNRDVRHC
                     HPPASAHIVDKIIEDFKTGKETRAPFWINMQGKLIHIEYFALRNEKGEYLGTLEVSQN
                     VQPYRDLEGEQRILSYAKK"
     gene            5180..5704
                     /locus_tag="PLN75_07485"
     CDS             5180..5704
                     /locus_tag="PLN75_07485"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRG03996.1"
                     /translation="MMETPKLIISPKTKVLQLIEAYPQLEDMLIEYVPTFRKLKNPIL
                     RNTIAKITTLQQAATIGNVKVEELINLFRGVVGQDAVELADEVTLNTTQPDWYDEDRI
                     VNSLDIRPMLNAGEQPVNQVVSDLNKLKPDEIYEVIAPFIPAPLIDKATSLNMAHWIE
                     RKGTELFLVYFCRK"
BASE COUNT         1953 a         1254 c         1280 g         1473 t
ORIGIN      
        1 aaaagcaaaa ttgccttgtc agctctgaat cttccacgcg gaacaaaaac cggatatctg
       61 aacttcagat tttcagcaac gatgatttcg ttgcggtgaa tggaagtttc tttgtgtccg
      121 gcatcaaaac tgttgctttc aaaacagaaa ttctttacat caatttttgc atcgggaagg
      181 tgtgtatcct tttccggagt gaaacaggta tttagtttta catatcggtc ggaatagttc
      241 attctttaat ttttatttga attgaatgca aaataataat atcaaaacag gatgtgcaaa
      301 aaaaagggaa atccatccaa tctcaggggt taaaaagcga gttgcgggat gaaaaccaaa
      361 atagcggggt gaaatttccg gtctgaaaac cgaagaatgg ataatttgac gtatttttgc
      421 accggataac aggcaacatc aggcaacaga tgaattcaaa aattcctcca tatctttacg
      481 agaaaggcaa taatgtccgt ctgattttat tcacggcatt ttttgccctg ctttttataa
      541 acatatttca gcctttcggg tcccggggct ggtatcccaa agtgtcggat ttgaagtatt
      601 tcgtgttttc gagtctgatt attctcaccg gaatgctggt agtggttgta agccggatga
      661 ttatgcaccg ctatactcac cgacacaaca tcagctacgt gcagtttgca atctggattt
      721 tgtgtgagat actggccatg tcgcttttct actcactttt taccaaattc atacccagcc
      781 agggtgccga aagacagttc tccgagatat tccgcagctg taccatcaat accggactca
      841 ttctgctgtt gccttactcc atttcgtggt tgtatttttc atggaaagaa aaaaaccgaa
      901 tgatagagaa actaaaggct gaagagtctg cccccgaagt tcatacccgg ccaatggtag
      961 cttttatgga tgaaaaagga gaattgaaaa tttcgctgat ggtcgaaaac cttctttata
     1021 tcgatgcagc tgataattat gccaccattt attacctcaa taagtcgaag ctttcgcatt
     1081 acctgctccg aaatacgctg aaatggatgg acgaaaacct cacaaacgat actccgctgg
     1141 tgcgttgtca tcgttcgtac atggtcaacc tggataaggt gaaagtactg cgtaagacca
     1201 aaaccggcat ttacatcgaa ctcgatgcag aaaatactcc cgacattccc gtctcaaaaa
     1261 catactacga aaggttcatg aaaaaatttt cgcagtactc ggtgtgatat tcccgggcga
     1321 ccatccccag ttgaggaaaa aaatccgggt cagtaaaatg ttttttttta gtgaatctga
     1381 aaaaacatct ttttttcttt taaaaaagac taaaatcaat taattatttg cgtatttcaa
     1441 ttaaaagttt ttcctttgca taaaataata caaacaggta gtatatagtg aaattatcta
     1501 aaacatcgga gtacgcattg cggatattaa tttacatggc ccaaaagccg gaagaattat
     1561 ataccgctaa gtttctggtg gagaaactga atgtgagtga caagtatctg cgacgcctga
     1621 tgaccgattt gtcaaaatca ggattcatca aaagtaccca gggacgcgac ggaggatata
     1681 cttttgccaa agaaatccat gagatatcat tatacgaagt gattgattcc gttgagggta
     1741 tgaacaagct gaacgactgt gtacttggct tcgacgaatg ttcgtgtacg aatccctgtg
     1801 ccatgcacaa cgagtgggtc gttgtcagag aaaaactgaa ccaactattt accgaaacca
     1861 ggttgtccga tctcgatttc gaaaggattt cacgttatta aattttttta tgcaaaaata
     1921 ctacatatta gtagtattat tgtaaacacg aacataaaca aaataacaaa aataaaatac
     1981 ctatccctaa aataaaaatt cagaacacac gacttgttct gatgcatata gggtagggaa
     2041 tgataattaa taaaactaac ttctctttat tcccccttca gagcttgtcc ctgccagtcg
     2101 ggaggggtag ggggcagata ttaaaactac aaaaaaatga aaacaattaa ctacaaagac
     2161 ctggtaactg ctcaaaaccc gcacggagta gatgcccgaa agctacattc aaacgacgat
     2221 gtgcaggttg tacacatgca cctgaaaccc ggacagagtc tgaaaaaaca tattaccgcc
     2281 gtgaatgtgt ttttctatgt gctggaaggt gaaggagtgg tggaaatagg agatgaaaaa
     2341 caaaccgtaa ccaaagattg ccttatcgac agtccgaaag acataccgca ctgcctctac
     2401 aacgaaagta cgaccgattt cagggtgttg gtggtgaaaa ctccatcgcc tacagctgca
     2461 caaaacacaa aagccatcga aaatatgttg aaacaatagt acaatacgat gaatgcaata
     2521 aaaattcttg ttgatgaaca cgacatcatt ctccgaatga tagatgttac ccgaagcatg
     2581 ctggtcgata catccacact caacacagac catgtagaac aggttgtgga ttttattaaa
     2641 aactttgccg ataaatacca ccacctgaaa gaagaggatg ttctgtttgt agaaatggaa
     2701 aaccacggca tgagccggca gagtggcccg gtgggagtga tgctgcacga acacgaacag
     2761 ggcagggcat acgtaaaaca ggcagaagaa ggtattctcc gactcaaatc aggcgatatc
     2821 catgcagccg gggcgattaa agaaagttta ctcaattaca gcacactgct caccaaccat
     2881 ataaataaag agaataatat tttgtacccg atggctgaaa gattgctgcc gcaaacagtg
     2941 atgctggcca tgtctgaaaa tttcggtgaa gccattacaa tcgcttcaaa caatgaattt
     3001 aaacaaaaat acctcaagat ggccgatgaa ctcagccgta tttatatgaa ataatgattt
     3061 ttttaaaaaa aacataacac gatgaaaaca aataaacaaa caaaaattgg tgacattgtc
     3121 accctggatt tcagagcagc cgaaatattc aaaaatgccg gcattgattt ctgctgtggt
     3181 ggcagtcaaa ccctcgggga tgcctgccgt gacaaaaatc tggatgtggc tgccatcgaa
     3241 acacaactgg agcaactcga aaattctgcg cccgactcgt cgcatatgtt caacgaatgg
     3301 aaactcgatt ttctgtgtga ctacatagtg aacacccacc atcaaacggt gatgaatctg
     3361 ttgccgcagt tgacctttta cacccaaaaa atagcagaag ttcacggcaa taatcatcct
     3421 gaactgcttg aaatagccgg tctttttgca caggtggata cagaactgcg ccagcacctt
     3481 cgtaacgaag aagaagtgct ttttccggcc atcaaagaag tgatgaaaac caattccgat
     3541 gaatcaaaag ccaccatcat cagcgaaatt accagaatga ccggcgaaca tgaatttgcc
     3601 ggaggcgcta tggacaaaat caatgagttg agccggcgct atgcggtgcc cgaagatgga
     3661 tgcaatacct accgcgtggc atacaagctg ctcgaacaat ttgaagatga tctgcatatt
     3721 cacgttcacc tggaaaacaa cattctgtat ccaaaggcga tgaaattgac ggtgtaaaac
     3781 tgaataaata gaaagaaagc tataattgta aacagaacgt aaaaccggat gtttctgtaa
     3841 acctaatccg gcaaagaaat gtttcattat tctaagttat catcgccccg aagacacaaa
     3901 acgataaatc aactttcaaa aaaaaacaga aatatgagtg aactaattaa caattcgcaa
     3961 aacagaaaag ataaacttaa agccctgatt ctgaaacttc acgaaggtga aacggaggaa
     4021 tcggtacgca acgaactgct tctgactctt agccagatac cctacggcga agtggtggag
     4081 gtagagcagg aattgatagc tgaaggcctg ccggaagagg aaatactcga cctgtgcgac
     4141 gctcattcgt cggtactcga gggcagggtt gacctttcgg cactgaaacc cgtgcccgac
     4201 gggcatccgg tacatgtgtt ccgccgtgaa aacgaggaaa ttcggaaaac caccaacgcc
     4261 atatttgcac gcctgtcgga tatcattcac ggaagtgaag aaaatctgca tgaaaaagtg
     4321 ctgaatcttc gcggattgtt caacagcctg tacgatgtgg acaaacatta tcaacggaag
     4381 gaatacctgg ttttcccgta tctcgaaaaa cttgaaatca ccggtccgcc caaagtgatg
     4441 tggggcaaac acgatgaaat acgcgaactg ataaaaggaa gcatagaagt actccaggtg
     4501 gacagcctca gcagggaaga gctcgaagca gcagccgata tggtgctgaa accggctgcc
     4561 cgcggcgctc aggaaatgat aaccaaagag gaagaaatac ttttccccat ggcgctcgat
     4621 gcactgaccg aggcccagtg gtacgaaata agcaggcaat ccatcgaaat tggtttctgc
     4681 ctttacgacc ccgaagtgga ctggcgtccg gaatgggtgg aagaacagac tgtcaaccag
     4741 ttgcagaaaa gcggaagcca tatacagttg cccagcggaa gtttctcggc cgaggaaatc
     4801 atggcgatac tcaatacact gccagtggat atgacattcg tggacaaaga cgacaaagtg
     4861 aagtatttct cgcagggagc cgagcgcatt ttccagcgca atcgtgccat cctcaaccgc
     4921 gacgtacgcc actgtcatcc gcccgcaagc gcacacatag tggataaaat tatcgaagat
     4981 ttcaaaaccg gaaaagaaac ccgtgcccct ttctggatca atatgcaagg caaattgatt
     5041 catattgagt actttgctct cagaaatgaa aagggagaat atcttggtac gctggaggtt
     5101 tcacaaaatg ttcagcccta cagggatctg gaaggtgagc agcgaatttt atcctatgca
     5161 aaaaaataaa taaaaaacga tgatggaaac acccaaactg attataagtc cgaaaacgaa
     5221 agtacttcag cttatcgaag catatccaca actggaagat atgctgattg aatacgttcc
     5281 gactttccga aagctgaaga atccgattct gaggaatacg atagccaaaa ttactactct
     5341 gcagcaggca gccaccatcg gcaatgtgaa agtggaggaa ctcatcaacc tgtttcgcgg
     5401 cgtagtgggg caggatgcgg tggagttggc cgacgaagtg actcttaaca ctactcagcc
     5461 cgactggtac gacgaagacc gcattgtcaa ctcactcgat atcagaccca tgctcaatgc
     5521 cggtgaacag cccgtgaacc aggtagtatc cgatctgaat aaactcaaac cggatgaaat
     5581 ctacgaagta atagctccgt ttattcccgc tccgctgata gataaagcta ccagcctcaa
     5641 tatggcacac tggattgagc ggaaaggtac agaactcttt ctggtttatt tttgcagaaa
     5701 ataaaagcac acttcggcag tcagctgtcc aatcaggcgg tttcggtatg atttcggaac
     5761 cgccttgtta gctttttaag atgaaaaatg attttaagat ttttgctttt tatttacaca
     5821 gtttattgaa cagacccgaa ttaccatagt tttttcaata ccgtaagttt aacgtgagtt
     5881 cgatataaaa ataaggacat ctacctgaat gaaagctttt taatttccca tagggaatta
     5941 atatcaatag taaaatatat
//
LOCUS       DAORKP010000129         5837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2809_length_5837_cov_9.837081, whole genome shotgun sequence.
ACCESSION   DAORKP010000129 DAORKP010000000
VERSION     DAORKP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_5837_cov_9.837081"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..594
                     /locus_tag="PLN75_07490"
     CDS             253..594
                     /locus_tag="PLN75_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRG03997.1"
                     /translation="MNLNQIIGLCDGEILNETSDIKDFRTAFSSDLMSDVLRWHSDNM
                     ILITGLATIQTIRTAEVSGISCIVLARGKKVSDEMLELAKESDIAIITSPASMFEISG
                     KLYENGIRPLY"
     gene            594..1004
                     /locus_tag="PLN75_07495"
     CDS             594..1004
                     /locus_tag="PLN75_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG03998.1"
                     /translation="MHLQFEIEGGDFSAAGEASSEVKKTLKQLNIPPVLVRRIAVALY
                     EAEVNVVAHAYRGVMEVDISGGGIYVKLKDKGPGIENIDRAMEEGFSTASDKVREMGF
                     GAGMGLPNIKKNCDEMKLTSTPGVGTTLELTINF"
     gene            1016..2398
                     /locus_tag="PLN75_07500"
     CDS             1016..2398
                     /locus_tag="PLN75_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRG03999.1"
                     /translation="MTGQHYVHAIQINTEKCIGCSHCMQVCSTQAIRIVEGHARIMPE
                     RCVDCGECYSVCPVAAVYVKDDELELNLQKFIYKIALVPAVFFGQFPTQYSVSQISSA
                     IRKLGFNAVFEVEHAVDFIKEQYVEIAEDAEIARPVISSFCPSVVRLIQVMFPSLTEN
                     ILRLKAPHDLAAIYLRHINSNSNINPEEIGVFYFTPCASKIVAAKAPEGEDDSPTDVV
                     VSMKLLYNKVYRIIKDIPLSDDLKRNSTMEPDSIKWALSGTEREYFKGRSLAIDGVEN
                     VIKFLEKLESGHITDIDFLELRVCDQGCAGGILCPGNRFLTVERIGERVKKLQNRLVE
                     NKEVVNPLMNHRELLEKGCKTEAIKPREGLLLDDNVEIALQKMKKIKALNSYLPGFDC
                     GACGAPTCKNLAEDIVKGKANMSYCVFVQRVMEKNYKLSPEQAFQTIEKIWGKDRLNK
                     YKDNHGEKES"
     gene            2379..2729
                     /locus_tag="PLN75_07505"
     CDS             2379..2729
                     /locus_tag="PLN75_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRG04000.1"
                     /translation="MEKKNLKKLIDDLGLTCVANENLLDREVNSAYVSDLLSDVMGKA
                     QPQMLWVTSQIHKNIVAVASLIELSAIVIVNERKPDEEMLKLAQEEGVVVLTSDNPAF
                     ETAGLLYNYLNTAP"
     gene            2790..3554
                     /locus_tag="PLN75_07510"
     CDS             2790..3554
                     /locus_tag="PLN75_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRG04001.1"
                     /translation="MDSESFIRNSSFRGRGGCTFKADLHIHTCLSPCGDLEMSPQNII
                     RTAKEKGLDIIAITDHNSTRNVRTCMELGKKNNLFVIGGCEINTQEEVHCLAYFPDLE
                     KLDEFQNFLDEHLPDIKNNPDIFGHQVAVDEDDNIIYEEEKALIMALDIDIESVSEKV
                     LSMSGIFIPAHIDRSKNSIFSQLGFIPFDLKYNALEISWRTTVEKLTEKYPKLTGKRF
                     ILSSDAHYPEDIGRVHTKLKMEKTDWESFVKAIHRL"
     gene            3597..4139
                     /locus_tag="PLN75_07515"
     CDS             3597..4139
                     /locus_tag="PLN75_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG04002.1"
                     /translation="MDIVQNSIRAGASNVEITFIENKTDSSLTFSVKDNGTGMTEETV
                     RKISDPFFTSRTTRKVGLGVPFLKMTCEQTGGSLTIQSELNKGTEITAVYRTDHPDCL
                     PLGDLAGYLVLLLIANPKMDIKFTYRLGDEKFEISARELGENEIDDLNSAGMSAAVKE
                     YINENLKELFLVRKTESYLC"
     gene            4176..4568
                     /locus_tag="PLN75_07520"
     CDS             4176..4568
                     /locus_tag="PLN75_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRG04003.1"
                     /translation="MEKIKTLADLKLKQEKFRSQVELRSKASRAEELVQIKVAMATCG
                     IASGAKEVMEFFVEELDKRNIEAVVTQTGCMGYCFAEPTIEVTLFGKDPVVFGYVDVK
                     KADEIIERYIKTGELVDGIIPKNYESIN"
     gene            4583..>5837
                     /locus_tag="PLN75_07525"
     CDS             4583..>5837
                     /locus_tag="PLN75_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRG04004.1"
                     /translation="MAKFKSHILICGGTGCQSSNSEAILNNLERELATKGLIEDVQVI
                     RTGCFGFCEKGPIVNVLPDNTFYVEVKPDDAQEIIDEHIIKGRRVNRLLYVNPENEEH
                     IPDSKHMGFYKKQLRIALRNCGLVDPENIEESIAHDAYQALGKVLTEMSQNDAIEIIK
                     KSGLRGRGGGGFPTGLKWEITKNSVSDKKYVVCNADEGDPGAFMDRSILEGDPHSILE
                     AMAICGYCIGADEGLIYIRAEYPLAIKRLKVAIKQAEEYGLLGSDIMGTGFNFKIKLR
                     YGAGAFVCGEETSLIHSMEGKRGEPTNKPPFPSVSGYLQKPTNVNNVETFANVPAIIL
                     KGAEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREIIFEIGGGIKGGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDYDNL"
BASE COUNT         1824 a         1070 c         1408 g         1535 t
ORIGIN      
        1 gttgcttcat ggggttattt gaaaaaaaaa gcggacaaaa aattctataa ttatgtataa
       61 tggttttaaa ttgtaagtga attccggtaa aaccttttaa aatttccttt ttttagatgg
      121 ttttatttag ttaatatact ggtaattaaa aacatagtta aatatcgaaa ataataagga
      181 aatatttctt taattaaaaa tcttgtttta tatttgtggt gaatttgtat aacataaatt
      241 aatagtagtc ttgtgaatct gaaccaaata atagggcttt gtgatggcga aattttgaac
      301 gaaacatctg atataaaaga ttttcgtaca gcattttcgt ccgatctgat gagtgatgtg
      361 ctgcgctggc acagtgacaa catgattttg ataaccggac tggctactat tcagacaatc
      421 cgcacagccg aagtttcggg aatatcatgc atagtgctgg ctcgtggaaa aaaagtgtcg
      481 gatgagatgc tcgaactggc aaaggaaagt gatattgcaa tcatcacgtc tcctgcttcc
      541 atgtttgaaa tttcaggtaa actctatgaa aacggtatta ggccgctata ctaatgcatt
      601 tacagtttga aatagaaggt ggagacttca gtgcagccgg tgaagcgagt tcggaagtaa
      661 aaaagacatt gaagcaactc aatattcctc cggttttggt acgcaggatt gcggttgcgc
      721 tttatgaagc tgaagtgaat gtggtggctc acgcataccg tggggtgatg gaggtggata
      781 tttcaggtgg tggaatttat gtgaagttga aagacaaggg acccgggatt gagaatatcg
      841 acagggcgat ggaagaaggt ttttctaccg catcggacaa ggtgcgcgag atgggatttg
      901 gagccggaat ggggctgcca aacatcaaga agaactgtga cgagatgaaa ctcacatcta
      961 ctcccggagt tggaacaacc cttgaattga caattaactt ttaaataata ccaacatgac
     1021 gggtcagcac tatgtgcatg cgatccagat caatactgag aagtgtattg gctgttcgca
     1081 ttgtatgcag gtttgttcca cacaggcaat tcgcatagtg gaaggtcatg cccgcatcat
     1141 gcccgagcga tgtgtcgatt gcggtgagtg ttatagtgtt tgtcccgttg cagccgttta
     1201 tgtaaaagac gatgaactgg agttaaatct gcagaaattc atttataaaa tagctttggt
     1261 accggctgtt ttcttcggtc agtttcctac ccagtattcc gtttcacaaa tttcatcggc
     1321 aattcgaaaa cttggtttta atgcggtttt tgaagttgag catgccgttg attttatcaa
     1381 ggaacaatat gtcgaaatag cagaggatgc ggaaattgca cgtcctgtga ttagttcttt
     1441 ttgtccatca gttgtacggc tgattcaggt catgtttcct tctctgaccg aaaatatact
     1501 tcgcttaaaa gccccacacg atctggccgc aatctatctg cggcatatca actccaactc
     1561 caatatcaat cctgaagaaa taggcgtatt ttattttaca ccctgcgctt cgaaaatcgt
     1621 ggcggctaag gcaccggaag gagaagatga ttcaccgaca gacgtggtcg taagtatgaa
     1681 gctcctttac aacaaagttt acagaataat caaagatatt cctttgtcgg atgatttgaa
     1741 gcggaactca accatggaac cggattcgat taaatgggca ctttctggta ccgaacggga
     1801 gtatttcaaa ggacgcagcc tggctattga cggagtggaa aatgtgataa aattcctcga
     1861 aaagctggag tcagggcaca tcacagatat cgattttctg gaattacgtg tgtgtgacca
     1921 ggggtgtgca ggaggcattc tttgcccggg aaaccgtttc ctgaccgttg agaggatagg
     1981 ggaaagagtg aaaaaattac agaacagatt ggttgaaaat aaagaagttg taaatccgct
     2041 gatgaatcac cgggagttgt tggaaaaagg atgtaaaaca gaggcgataa agccccgaga
     2101 agggttgtta ctggacgata atgtggaaat cgcactgcag aaaatgaaaa aaatcaaagc
     2161 gctgaattcg tatcttcccg gatttgactg tggagcctgc ggtgcaccta cctgtaaaaa
     2221 tctggcagaa gacatcgtga aagggaaggc aaatatgtcg tattgcgtat ttgtacagcg
     2281 ggtgatggaa aaaaactaca aactcagtcc ggaacaggcg ttccagacca ttgaaaagat
     2341 ttggggtaaa gacagactta ataaatacaa agacaatcat ggagaaaaag aatcttaaaa
     2401 agctcattga tgacctcggt ttaacgtgtg tggcaaatga aaatctgctc gatagagaag
     2461 taaattcagc atatgtgtct gatttgttga gcgatgtgat ggggaaggca cagccacaga
     2521 tgctgtgggt aacatcacag attcataaaa atattgtagc ggttgcttcc ctgattgaat
     2581 tgagcgccat tgtaattgta aatgaacgca agcccgatga agaaatgttg aaactggctc
     2641 aggaagaagg agttgttgtg cttacgtctg acaacccggc ttttgaaaca gcagggcttt
     2701 tgtataatta tttgaatacg gccccctaaa tcccccgcaa ggggacattt tgagcgtggt
     2761 actatgatga ttagattaaa tacagaaaat tggactctga atccttcata agaaattcct
     2821 ccttcagggg gcgagggggc tgtactttca aagccgacct gcacattcac acgtgccttt
     2881 ctccttgtgg tgatctggag atgagtccgc agaatattat ccgaacagcc aaagaaaaag
     2941 ggctggatat catagcaatt actgaccaca actccacccg caatgtaaga acgtgcatgg
     3001 agttgggtaa aaaaaataat ctgttcgtca tcggcggatg cgaaataaat acgcaggaag
     3061 aggtgcactg cctcgcttac tttcctgatt tggaaaaact ggatgagttt cagaatttct
     3121 tggatgaaca tttaccggac ataaagaata atccggatat ttttggacat caggtggcag
     3181 tagatgaaga tgacaatatt atatatgaag aagaaaaagc gctgattatg gctctggata
     3241 ttgatatcga gtcggtttct gaaaaagttt tgtcaatgag tggaattttc attcccgcgc
     3301 acattgacag gtctaaaaac agtattttta gccagctcgg gtttattccg tttgatttga
     3361 aatacaatgc actggagatt tcctggcgta caactgttga gaaacttact gaaaaatacc
     3421 caaaattgac cggaaagaga tttatactaa gttcggatgc tcactatccg gaagatatag
     3481 ggagagtgca tactaaactg aagatggaaa aaacagattg ggagagtttt gttaaggcaa
     3541 ttcataggtt gtaaaataat tgtacaaaga agttggttga tcttgcaatg catatgatgg
     3601 acattgtcca aaactcaatc cgggctggag cctcgaatgt ggaaatcacg tttatagaaa
     3661 ataaaacgga tagcagtttg acattttctg taaaagataa cggtaccgga atgacagaag
     3721 agactgttag gaaaatttct gatccttttt ttacttcccg aactactcga aaggttggat
     3781 taggtgtacc tttcttaaaa atgacttgtg agcaaacggg tggctcgctg acaattcagt
     3841 cggaactgaa taaaggaacc gaaattactg ctgtttacag aacagatcat ccggattgtc
     3901 tgcctctggg cgatctggcc ggttatctgg ttttactgct gatagccaat ccgaaaatgg
     3961 atataaaatt cacctaccga ttgggtgatg aaaagtttga aattagcgcc agggagctgg
     4021 gcgaaaatga aattgatgac ttaaacagtg ccggtatgtc cgccgctgta aaagaatata
     4081 tcaatgaaaa tctgaaagaa ttgtttttag ttcgcaagac cgaaagttat ttgtgttaag
     4141 aattaaatat taactatatt tcaatcaaaa caaaaatgga aaaaataaaa acattggctg
     4201 acttgaaact gaaacaggaa aagttcagat ctcaggtaga actgaggagt aaagcaagcc
     4261 gggccgaaga acttgtacag ataaaggtag cgatggctac ctgcggcatc gcatccggcg
     4321 ccaaggaagt gatggagttt tttgtagaag aactggacaa aagaaatatc gaagctgtgg
     4381 tgactcaaac cggttgtatg ggatattgct ttgcagagcc gacaatcgag gtgacacttt
     4441 ttggaaaaga tccggtagtc ttcggttacg tagatgtgaa gaaagccgac gaaatcatcg
     4501 aacgctacat caaaaccgga gaactggtag atggaattat acccaagaat tacgaatcaa
     4561 ttaattaagg gaggacattc gcatggcaaa attcaaatca cacatcctga tttgtggtgg
     4621 tacaggttgt caatcatcga acagtgaagc cattttaaat aatctggaaa gagagttggc
     4681 taccaaagga ctgattgaag atgttcaggt aattcgcacc ggctgtttcg gcttctgtga
     4741 aaaaggacct atcgttaatg tattgccgga caatactttc tatgtggaag tgaaaccgga
     4801 tgatgcacaa gaaatcatcg acgaacatat catcaaagga cgccgtgtga accggttgct
     4861 gtatgtaaat ccggaaaacg aagagcatat ccccgactcc aaacacatgg gattctacaa
     4921 aaaacaattg cgtatcgctt tgcgtaattg cggtttggtg gaccctgaaa atatcgaaga
     4981 atccattgca catgatgctt atcaagcgct tggaaaagtg ttgaccgaaa tgtcgcagaa
     5041 cgatgccatt gaaatcatta agaaatctgg cttgcgcgga cgtggcggcg gcggattccc
     5101 gaccggactc aaatgggaaa tcactaaaaa cagtgtttcg gataagaaat atgtggtttg
     5161 taatgccgac gaaggcgatc ccggtgcatt tatggaccgt tccatcctcg agggagatcc
     5221 gcactccatc ctcgaagcca tggccatttg cggttattgc ataggtgctg atgaagggct
     5281 gatttacatc cgcgctgaat atccgctggc tatcaaacgt ctgaaagtgg ccatcaaaca
     5341 agctgaagaa tacgggcttc tgggtagcga catcatgggt accggcttca atttcaaaat
     5401 taaacttcgc tacggagccg gagctttcgt gtgcggtgaa gagacatctc tcattcactc
     5461 catggaaggc aagcgtggcg agcctaccaa taaacctcca ttcccgtccg tgagcggtta
     5521 cctgcaaaaa ccgaccaacg taaacaacgt ggaaacattc gccaatgtgc ctgctattat
     5581 tctgaaaggc gcagaatggt tctcttccat tggtacggaa aaatccaaag gaacaaaagt
     5641 ttttgctctg gccggaaaaa tcaacaacgt tggactgatt gaagtgccga tgggaattac
     5701 acttcgtgaa attatattcg aaatcggcgg cggcatcaaa ggaggtaaaa aattcaaagc
     5761 cgttcagacc ggaggccctt cgggaggttg cctcaccgaa aagcacctgg acaccccgat
     5821 cgactacgac aacctgc
//
LOCUS       DAORKP010000130         5825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2819_length_5825_cov_7.972270, whole genome shotgun sequence.
ACCESSION   DAORKP010000130 DAORKP010000000
VERSION     DAORKP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_5825_cov_7.972270"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /gene="nifJ"
                     /locus_tag="PLN75_07530"
     CDS             complement(<1..2102)
                     /gene="nifJ"
                     /locus_tag="PLN75_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRG04005.1"
                     /translation="MAKEKKFITCDGNQAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAASGRKNMFGETVLVEEMQSEGGAAAAMHGTLQTGTLATTFTASQGLLLMIPNMYKV
                     AGELLPGVYHVSARAIAAQALSIFGDHQDVMAVRQTGCALFATGSVQEVMDMAAVAHL
                     AAIKSRIPFVHFFDGFRTSHEIQKIEEIDAKAVQALVDQDALTEFRKRGLNPENPVVR
                     GTAQNPDIYFQSREAANPFYDALPGIVEEYLDQLAVITGRKYGLFDYYGDPEADRVVI
                     AMGSATEAIREGIDHLMEKGEKVGLVSVHLYRPFSAKHFLSVLPKSAKRICVLDRTKE
                     PGANGEPLYLDIKDALYGTENQPLVVGGRYGLSSKDFTPAQVFAVYKNLALPTPRNRF
                     TVGIVDDVTFTSLPLGEEISLGGEGTYEAKFYGLGADGTVGANKNSIKIIGDNSNKYS
                     QAYFEYDSKKSGGFTCSHLRFGDSPIRSTYLVTTPNFVACHVQAYLRLYDVTKGLKKN
                     GTFLLNTVWNAEEVKDNLPLNIKRYFAQNNIKLYIINATDIAHEIGLGNRTNTILQSA
                     FFKITNVIPYDLAVEQMKKAIVKSYGRKGEVIVNKNYAAVDRGGEYVEVEIPSEWANL
                     ADEVEVNDNVPAFIRDIVRPVNAQNGNSLPVSVFKGREDGTWIQGTTKYEKRGVAAFV
                     PEWDLNNCIQCNQCAYVCP"
     gene            complement(2227..3405)
                     /locus_tag="PLN75_07535"
     CDS             complement(2227..3405)
                     /locus_tag="PLN75_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG04006.1"
                     /translation="MEGFYKTHQYLVQHVESPVRRLLMDEIDWSHRLIGIKGSRGVGK
                     TTFLLQYAKDHFEPTDRRCLYVNFNNLYFTTHRLVDFAGDFYAAGGRILLLDQIFKYE
                     NWSKELRYCHDKYSELQIIFSGSSVMRLVEDNEDLQDVVASYNLRGFSFREYLNLKSG
                     NNFQHFSLEEIIQNHTDISNKICNIVNPMNYFHEYLHHGFYPFFLESRNYSENLLKTI
                     NMMMEVDILLIKQIDLKYLSRIRKLFYLIMQCAPCTLNVSQLSDEIQTSRSTVMNYIK
                     YLRDARLLNWIYAEGDDFPKKPKAVFPQNTNIMHVMLNNVDKVCEQRTFLYNALHARH
                     KVNLGKKHADFLIDNALSFKYIERLKNQKNNPNTLYAVSSITTGAKNEIPVWLFGFLY
                     "
     gene            3752..5179
                     /locus_tag="PLN75_07540"
     CDS             3752..5179
                     /locus_tag="PLN75_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRG04007.1"
                     /translation="MELGKTDNRTMQHLDILANRIGGRPTGSDAYNTATYWVAGLLEK
                     WGLEVEIQEAGTVPVGFNRGPWFGRAIGSLNLTLEFVTPSYTSGTRGIQRGHVVIEPK
                     TRQEFERMKGTLKGAWVLIEGKNNGFPVDYSAEGDSIREAVIKHNSEAKSDTAMKTEP
                     ALFYKEMKAAGVLGIIQSAPVPLVAVYDKKNLMKMSFENLPDLPDIKLNEEQYQLIRQ
                     KVEKREFVMLEFDIRNHFKPGPVKYHNVIGKIKGVKFPDEYVISGCHLDSHDAASGAV
                     DCGTGVAPNLEMARMIMAAGGNRPDRTILFCFWAGEEYGFLGSKYWVEHNMDKLPRIS
                     NYFNRDGGPTAASGLVVPEKMVEDILKCTEGLENYDPKIPFRVETSKIHPVNIPSKPG
                     YTDEAYFAMNGVPTLQFKNSDPLGYNFSYREIWHTNRDLYNMSIPEYMNYTSVIQAVT
                     IYNIANLKNLLPRSEIYKQKITQGK"
     gene            5211..5699
                     /locus_tag="PLN75_07545"
     CDS             5211..5699
                     /locus_tag="PLN75_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRG04008.1"
                     /translation="MKTLIIFRHGKAESYDAKPNDYDRELAGRGRLNAGEMGKFILKK
                     YGKPGLVLASSARRTLQTATLAAEEMEYPITEIKTDENLYLASERRIMKIIRSLPDDC
                     ESCLLVGHNPGLTNLINQLNVRLDNLPTGSAVCFNFNTGKWSEISADNSQFQWLQLAR
                     EL"
     gene            complement(5723..>5825)
                     /locus_tag="PLN75_07550"
     CDS             complement(5723..>5825)
                     /locus_tag="PLN75_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRG04009.1"
                     /translation="AEKKYAAHDFADVAYFEPFYLKEFQATIPKKLF"
BASE COUNT         1760 a         1173 c         1236 g         1656 t
ORIGIN      
        1 ggacaaacgt aagcacactg gttacattgg atacagttat tcaaatccca ttcaggaacg
       61 aatgctgcca caccacgttt ttcgtatttg gtagttcctt ggatccaggt tccgtcttca
      121 cgacctttga atacagaaac aggcagactg ttaccgttct gagcgttaac cggacgaaca
      181 atgtcgcgga taaatgcagg tacgttgtcg tttacttcta cttcatctgc cagatttgcc
      241 cattctgacg gaatttctac ttctacatat tcaccaccac ggtcaacagc cgcgtagttt
      301 ttatttacaa ttacttcacc ttttcttccg tatgatttca caatggcttt tttcatttgc
      361 tcaaccgcca aatcgtaagg aattacattg gtgattttga agaatgctga ttgtagaatg
      421 gtgttggtgc ggttacccag accaatttcg tgtgcaatgt cagttgcatt gatgatataa
      481 agcttgatgt tgttttgagc aaaataacgt ttgatgttca gcggaagatt gtctttcact
      541 tcttcagcat tccaaacagt attcagcagg aatgtgccgt tctttttcag gcctttcgtt
      601 acatcgtaca gacggaggta agcctgaacg tggcaagcaa cgaagtttgg agtggtaacc
      661 aaataggtag aacggatagg agagtcgcca aaacgcaagt gcgaacaggt gaaacctccc
      721 gatttcttgg agtcgtactc aaaataggct tgagaatatt tattggaatt gtcaccaatg
      781 attttgatgg agtttttgtt agctccaaca gttccgtcag caccaagacc gtagaatttc
      841 gcttcgtatg taccttcgcc acccagtgaa atttcttcac ccaatggaag agaagtaaat
      901 gtaacatcat cgacaatacc tactgtaaaa cggttgcgcg gagtaggcaa tgccaggttt
      961 ttgtaaacgg caaatacctg agctggagta aagtctttgg atgaaagccc gtaacgtccg
     1021 cctactacaa gcggttgatt ttctgttccg taaagagcat ctttaatatc taaataaaga
     1081 ggttcgccgt tagctcccgg ttctttggta cggtcaagta cacaaatccg ttttgcagat
     1141 tttggaagta cagaaaggaa atgttttgcc gagaaaggac gatacaagtg aacagaaaca
     1201 agaccaactt tttcgccttt ttccatcaag tggtcaattc cttcgcggat agcttcggta
     1261 gcagaaccca tagcaattac tacgcgatca gcttcaggat ctccgtaata atcaaacagt
     1321 ccgtatttac ggccggtaat tacagctaat tgatccaaat attcttctac aattccagga
     1381 agtgcgtcgt agaaaggatt agcagcttcg cgagattgga agtaaatatc cgggttttga
     1441 gccgtaccac gaacaacagg attttcagga ttcaaaccgc gtttgcggaa ttctgtcaga
     1501 gcatcctgat caaccaatgc ctgaacagct ttggcatcaa tttcttcgat tttctgaatt
     1561 tcgtgagaag tacggaatcc gtcgaagaag tgtacaaatg gaatgcggga tttgatagca
     1621 gccaagtgag ccactgcagc catatccatt acttcctgca cagaacctgt tgcaaataat
     1681 gcacaacctg tctggcgtac agccataacg tcctgatggt cgccaaagat ggaaagtgcc
     1741 tgagcggcaa ttgcacgggc agatacgtga tatacgcccg gaagcagctc gccggccact
     1801 ttgtacatat taggtatcat cagaagcaat ccctgagaag cagtaaaggt agttgccaat
     1861 gtaccggttt gaagcgtgcc gtgcattgct gctgccgctc caccttctga ttgcatttct
     1921 tcaacaagca ctgtttcacc aaacatattc tttctaccgg aagccgccca ttcatcgacg
     1981 tattcagcca tagtagaaga aggtgtgatt ggataaattg cagccacttc gctaaacatg
     2041 taggcgatat gagcggcagc ctgattacca tcacaggtaa taaatttctt ttcttttgcc
     2101 atttattaat ttttttagtt agtttatttc aagcagtatg taatcaataa tattcgtttt
     2161 ttttattgta aaacaaccga aagcagggat ttaaaagttt tcaataatcg tcattcagtc
     2221 aatcgtttaa tacaaaaaac caaataacca taccggaatt tcattcttcg ctcccgttgt
     2281 tatgctgcta acagcataca gcgtattcgg attgtttttt tgatttttca gacgttcaat
     2341 atacttgaag ctaagtgcat tatctatcag aaaatcagcg tgttttttgc ccagattcac
     2401 cttgtggcgt gcatgtaatg cattgtaaag aaacgttctt tgctcgcaga ctttatcaac
     2461 attgtttaac attacatgca tgatatttgt gttttgcgga aaaacggctt ttggtttttt
     2521 cggaaaatcg tcaccttcag cataaatcca attcagcaaa cgcgcatctc tgaggtattt
     2581 aatataattc attacagttg aacgggaagt ttgaatttca tcactcaact ggctaacatt
     2641 cagtgtgcaa ggtgcgcatt gcataatcag ataaaaaagt tttctgatcc gtgaaagata
     2701 cttcaaatct atctgtttga tcagtaaaat atcaacttcc atcatcatgt taatggtttt
     2761 cagcaaattt tcggaatagt ttctggattc cagaaaaaaa ggataaaaac cgtgatgaag
     2821 gtattcatga aagtaattca tcggattcac aatattacaa attttatttg aaatatcggt
     2881 atgattttga ataatttctt cgagcgaaaa atgctgaaaa ttgtttccgg atttcagatt
     2941 cagatattca cggaaagaga aaccacgcaa attgtaagat gctaccacat cctgaagatc
     3001 ttcgttgtct tccaccagtc gcataaccga tgagcccgaa aaaatgatct gcagttcaga
     3061 gtacttgtcg tggcaatatc tgagttcttt cgaccagttt tcatacttga aaatctggtc
     3121 gagaagcaga attctccctc ctgcagcata aaaatcaccc gcaaaatcaa ccagtcgatg
     3181 agtggtaaag tacagattat tgaaattcac atacaggcag cgtcggtcgg ttggctcaaa
     3241 atgatcttta gcatattgga ggaggaaggt agtcttacct acaccccgac tgcctttgat
     3301 gccaatcaga cggtgcgacc agtcaatttc atccatcaga agtcttctga caggtgattc
     3361 gacatgttga accaaatatt gatgcgtttt gtaaaaacct tccatggatg atgataaatc
     3421 gaatacaaaa ttatataaaa aaattcattt tgcaaagtgg agtttgcaaa atgaacaaaa
     3481 atacacacgt ttttatattt ctgatttgat ttaaaaacaa ttggtttcac agaaatattc
     3541 aaaattaaaa ttttttcaat tcaggaaacc ccttgataga attcaaaaaa ataaggaatt
     3601 aatccgaatt ttcctgaaat tttgctttat ctttgaaacc tgaaatagtt ttaaatcata
     3661 tattatgtac aactcaaagc gacttttttt attcttagtc atatttttat ctttcgcagc
     3721 cggttcacaa aataaagtat ccaaacgaat aatggaactt ggaaaaaccg ataaccgcac
     3781 catgcaacac ctggacatcc tggcaaaccg aataggtgga cgacccacgg ggtctgatgc
     3841 ctataacaca gcaacgtact gggtggcagg attgctcgaa aaatggggac tcgaagttga
     3901 aattcaggaa gcgggaactg tgcccgttgg gtttaaccgt ggtccctggt ttggcagggc
     3961 tattggcagt ctgaatttga cgctggagtt tgttacgcca agttacacat caggcaccag
     4021 gggaatacaa cgtggacatg tagtgattga accaaaaacg cggcaggagt ttgaaagaat
     4081 gaaaggtaca ctgaaaggag cctgggtgct tattgaggga aaaaataacg gtttcccggt
     4141 tgattactcg gccgaaggcg attccatcag agaagctgtg attaaacaca actccgaggc
     4201 taaatcagac actgcaatga aaaccgaacc agctctgttt tacaaggaaa tgaaagctgc
     4261 aggcgttttg ggtattatac agtcggctcc tgtgccgttg gtggccgtat acgacaagaa
     4321 aaatctcatg aaaatgagtt ttgagaatct tccggacctg cctgatatta agttgaacga
     4381 agaacagtat caattaatca ggcagaaggt tgaaaaacgc gaatttgtga tgctggagtt
     4441 tgacattcgc aatcatttca agcccgggcc ggtaaaatat cacaacgtga tcgggaaaat
     4501 aaaaggggtt aaattccctg atgaatatgt aataagcggt tgtcatctgg attcgcacga
     4561 tgctgcatct ggtgcggtag attgcggaac gggcgtagca cccaatctgg aaatggcgcg
     4621 aatgataatg gctgccggtg gaaatcgtcc ggatcgtacc attttgttct gtttctgggc
     4681 gggtgaggaa tacggctttc ttggttcaaa atactgggtg gaacacaaca tggataagct
     4741 tccgcgaatt tccaactatt tcaaccgcga tggaggtcct acggctgcat caggtcttgt
     4801 agtgcccgaa aagatggttg aagatattct aaaatgcacc gaaggactcg aaaattatga
     4861 cccgaaaata ccgttcaggg tcgaaacgag taaaatccat cctgtgaaca ttccttcaaa
     4921 accaggatat accgatgagg cttacttcgc tatgaatggt gtacctacat tgcagtttaa
     4981 gaattctgat ccgcttggtt acaacttcag ttaccgcgaa atatggcaca ccaaccgcga
     5041 tctttacaat atgagtattc cggagtatat gaattatact tcagtaattc aggcggttac
     5101 aatttataat atagccaatt tgaaaaatct gcttccaaga agtgaaattt ataaacaaaa
     5161 aattactcag gggaaataat tttctcacga tataatcgtt cgaagcgaca atgaaaacat
     5221 taatcatttt tagacacgga aaagccgaat catacgatgc taagcccaat gattatgacc
     5281 gtgaacttgc cggcagagga aggctcaacg cgggtgaaat gggtaaattt attcttaaaa
     5341 aatacggcaa acccgggctg gtgctggctt cgtcggccag gcgaacgctc cagactgcca
     5401 cccttgccgc cgaagaaatg gagtatccga ttacggaaat aaaaaccgat gaaaatctct
     5461 atcttgcttc tgaaagaaga atcatgaaaa taatcagaag cctgcctgat gattgtgaaa
     5521 gctgtctgct ggtagggcat aatcccggac tgaccaatct gatcaatcaa ctgaatgtga
     5581 ggcttgacaa cctgcctaca ggctctgcag tctgctttaa cttcaatacc ggaaagtgga
     5641 gtgaaatttc tgctgataat tcgcagtttc aatggctcca gttagcccgc gagctataga
     5701 tgaaccagta ttttttgttt tttcaaaaaa gttttttcgg tatggtagcc tgaaattctt
     5761 tgaggtagaa aggctcaaag taagctacat cagcaaagtc atgtgcagca tatttttttt
     5821 cggcc
//
LOCUS       DAORKP010000131         5823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2821_length_5823_cov_8.486997, whole genome shotgun sequence.
ACCESSION   DAORKP010000131 DAORKP010000000
VERSION     DAORKP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_5823_cov_8.486997"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLN75_07555"
     CDS             complement(<1..767)
                     /locus_tag="PLN75_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRG04010.1"
                     /translation="MFGFLKSFDRKDHHPILGGLDIFKYIGPGLLVTVGFIDPGNWAS
                     NLAAGADFGYALLWMVTLSTIMLILLQHNVAHLGIATGLCLSEAATVHLKPRYSRLLL
                     ISGMGASVSTSLAEILGGAIALNMLFNVPIRAGALLVMIFVLIMLFTNSYKAIEKWII
                     AFVSIIGLSFLYELSLVNIDWNSALTGWVTPTFPKGSMLVIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNREDETVIKKQLKYEFFDTLMSMLIGWAI"
     gene            complement(811..2916)
                     /gene="ovoA"
                     /locus_tag="PLN75_07560"
     CDS             complement(811..2916)
                     /gene="ovoA"
                     /locus_tag="PLN75_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HRG04011.1"
                     /translation="MEHLITRTIDLQHGNPDEKRKEIRDYFLKTWEIDEKLYTQLKSD
                     DIFYHRGDPLRHVILFYLGHTAVFYINKLMLAKMIDTRINPEFESIFAIGVDEMSWDD
                     LNEKHYNWPQVSAVREYRNKAKDLILSVIDNAPLSMPVTWNQPFWIIMMGIEHQRIHL
                     ETSSVLIRQLPLNEVVAGRFGERCPDSGSAPENEMINVTGAKVILGKSENHPFYGWDN
                     EYGKYEEHVEDFQASKYLISNGEYLKFIHDGGYQTEEYWTEEGWNWRNFKEAEMPLFW
                     RKNGEDYILRLVAEEIPMPWNWPAEVNYLEAKAFANWKTKTTGKIHRLPTEAEWYRLH
                     EAAGLKDVTDWQKAPGNINLEYCTSPCPVDKFKQGDFYDVVGNVWQWTETPITGYPGF
                     KVHPLYDDFSTPTFDGKHNLIKGGSWISTGNEATSHARYAFRRHFYQHAGFRLVESAH
                     PLVIQSNEYETDQEVTLSCESNWGDSFIAAPIFSKVLARLVKDVAGDNRTLRLLDLNA
                     DSGRLAFELAQYYDDVTALDFTARMIRIPIQLQEQGFMRYVMKDEGELQFFRDVVLSD
                     YGLESGKNNILFMQADAMNLKPLYSGYDIIVVPNLLEELNNPILFLKKIHERLNNNGL
                     LVIASTYEWDDTITTRENWPGGFKQDGEPVTSLDGIKQILSEKFDIFGDPIDLNIYQK
                     LSSRISVHKISQLTFWKKR"
     gene            complement(2919..3470)
                     /locus_tag="PLN75_07565"
     CDS             complement(2919..3470)
                     /locus_tag="PLN75_07565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRG04012.1"
                     /translation="MNHTEIKYTIEKLTPLYYSDWKEELLELYLHAFTTGEFAQYVRR
                     EDASEKLDEFQRVGSGAVAFLHGKPVGALMGVPFTSDAEFPFANHPEIPVKTTAYIAE
                     LMVHVHVRGLGIASALIHEYLKNETLNGVTDAVIRVWIENIPAVKLYEKLGFRRIEEI
                     TQTKYKSETETFEMQKIYLHKKL"
     gene            3619..4626
                     /locus_tag="PLN75_07570"
     CDS             3619..4626
                     /locus_tag="PLN75_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04013.1"
                     /translation="MRKQKILYLSFFISIVLVSCGGKTGKGAESESVTEAEHPQFVDS
                     TAFHQKLTQLANGDTTGLWPVKNQPIPLYGALLPEHRIVAYYGNLYSKKMGALGEYAP
                     KEMWSRLKAEVKAWEAADPTTPVIPALHYIAVVAQGDPGKDGKYRFRMPEKQIDSVFT
                     ISRMHPGTLVFLDIQVALSKIQDELPLLEKYLKLPNVHLGIDPEFSMKDGSKPGKRIG
                     TYDAKDVNYCSQYLAKLVKEHNLPPKVFVIHRFTQGMITNYKNIRLHPEVQIVIDMDG
                     WGEPELKKGTHRNYIHKEPIQFTGFKLFYKNDLKKSPNRMMTPQELLKLRPQPSYIQY
                     Q"
     gene            complement(4785..5822)
                     /locus_tag="PLN75_07575"
     CDS             complement(4785..5822)
                     /locus_tag="PLN75_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HRG04014.1"
                     /translation="MSVLSYSQVRNEIQIPDILGFKTLKCDFHMHTVFSDGLVWPTVR
                     VSEAYSQGLDAIAITDHIEYRPHKNDMPGASLNRPYEIAEAAARSNNIILIRGSEITR
                     SMPPGHSNALFLSDCNALDVSVYMDAFKAAKAQNAFIFWNHPGWDAQQPDTTRWFKEH
                     TELYNAGLMQGIEVVNGTEYYPEAQRWCMEKKLTMLGNTDVHDPLTPKEGEHRTMTLV
                     FASARTPEAIHEALIERRTVVYLNDKLIGEEKYLKEIFHQSIEIVNTEKTDKYYRITV
                     KNNSDLPFYLHKTSHNPDLVYFRDYTIDAHGRHTITVKLANGATGGDVNFEITNLLAE
                     PNKGLHVSFPI"
BASE COUNT         1599 a         1327 c         1256 g         1641 t
ORIGIN      
        1 ttgatagccc agcctatgag catggacatg agtgtatcga aaaattcgta cttcaactgt
       61 ttcttaatca cggtttcgtc ctccctgttc cactgacggc tctgaatgac ttcggagtga
      121 agaaaaagat tatgaggcat cactacggct cccagtacac tcataatcac caacatggaa
      181 cctttcggga aagtgggcgt cacccaaccg gttaatgcgc tgttccaatc gatattcaca
      241 agcgacagtt cgtacagaaa cgacagtccg atgatggaaa cgaaggcaat aatccacttt
      301 tcgatggctt tgtatgagtt agtaaacagc atgattagca caaaaatcat caccagcagc
      361 gctccggcac gaatgggaac attgaataac atattcaggg caatggcacc accaagtatt
      421 tcggcaagtg acgtagacac agatgccccc atgcccgaaa taagcaatag cctggaatag
      481 cggggtttca gatgcacggt ggcagcttcc gaaaggcaaa gccccgtggc aatgccgagg
      541 tgagccacgt tgtgttgcag gagaataagc atgatggtgg aaagcgtgac catccacagc
      601 aaggcataac caaaatcagc tccggcagcc agattggaag cccagttgcc cggatcgata
      661 aaacctaccg taaccagcaa tcccggacct atgtatttga aaatatccag gccgcctaaa
      721 atcggatggt gatccttcct gtcaaaactc ttcaaaaatc caaacatagt tatattctgt
      781 aatcttatat taatgaaacg gattgataca ctatcttttt ttccagaagg ttaactgaga
      841 tatcttatga acagagatcc ggctggataa tttctgataa atattcagat caatggggtc
      901 tccgaagata tcaaactttt cactcagtat ctgcttgatt ccatcgagcg aagtcaccgg
      961 ttcgccatcc tgcttgaaac ctcccggcca gttttcgcgt gttgttatcg tatcatccca
     1021 ttcatacgtg gacgcaataa ccagtaaacc gttattattc aggcgttcgt gtattttttt
     1081 caggaataaa atcggattgt tcagttcttc gagcagattc ggaaccacaa tgatatcata
     1141 accggaatat aatggtttca gattcatagc gtcagcctgc ataaagagaa tattgttctt
     1201 tccggactcg agtccataat ccgataaaac cacatcgcgg aaaaactgca attcgccttc
     1261 atccttcatc acataccgca tgaatccctg ctcctgaagc tggatgggaa tacgtatcat
     1321 ccgggcagta aagtcgagtg ctgttacatc gtcgtaatat tgggcaagtt caaaagccag
     1381 ccttccggaa tcggcattca aatcgagtaa acgcagtgtg cggttgtcac ctgccacatc
     1441 ctttacgagg cgggcaagta ctttcgaaaa aatgggagct gcgataaacg aatctcccca
     1501 gttggattcg cacgaaaggg taacttcctg atcggtttca tattcgttgc tttgtattac
     1561 cagcggatgc gcggactcta cgaggcgaaa acctgcatgc tgatagaaat gtctgcggaa
     1621 ggcatatcgt gcgtgtgagg tggcttcgtt tccggtggaa atccacgatc ctccttttat
     1681 cagattgtgt tttccatcga aagtcggagt agaaaaatcg tcgtacaacg ggtgcacttt
     1741 gaagccggga tagccggtta tgggtgtttc ggtccattgc cacacgttgc ccactacatc
     1801 gtaaaaatct ccttgcttaa atttatccac cggacagggt gaagtgcaat actccaggtt
     1861 gatgtttccg ggagcttttt gccagtctgt cacatctttc aatcctgctg cctcgtgaag
     1921 tctgtaccat tctgcttcgg tgggcaaccg gtggattttt cctgtggttt ttgttttcca
     1981 gttggcaaaa gcctttgctt cgaggtagtt gacctctgca ggccagttcc acggcatagg
     2041 aatctcttca gccaccagcc tgagtatgta atcttccccg ttttttctcc agaacaaagg
     2101 catctcagcc tctttgaaat ttcgccagtt ccagccttct tcagtccagt attcttcagt
     2161 ctgataacct ccatcgtgaa taaatttcag gtattcaccg ttggaaatga ggtatttcga
     2221 tgcctggaaa tcttccacat gctcttcgta ctttccatat tcgttgtccc atccgtaaaa
     2281 cgggtgattt tcggattttc caaggataac ttttgcacct gtcacattta tcatttcatt
     2341 ttcgggagca gaaccgctat caggacatct ttcgccaaat cttccggcaa caacttcgtt
     2401 caaaggcagc tgacggataa gcaccgacga cgtttcgagg tgaatgcgct gatgctcaat
     2461 gcccatcatg atgatccaga aaggttgatt ccaggttacc ggcatcgaca aaggggcatt
     2521 atctatcacg ctcaggatta ggtccttagc tttattgcga tattcgcgta cggcgcttac
     2581 ctgaggccag ttatagtgct tctcgttcag gtcgtcccaa ctcatttcat ccacgccaat
     2641 ggcaaatatc gattcgaact ccggattaat acgggtatct atcattttag ccagcatcaa
     2701 cttgttgata taaaacacag ccgtatgtcc gaggtagaaa agtatcacgt ggcgcagcgg
     2761 atcgccgcgg tgataaaaaa tatcgtcaga tttcagctgg gtgtacaatt tctcgtcaat
     2821 ctcccatgtc ttgaggaaat aatcgcgaat ttcctttcgc ttttcgtcgg gattaccgtg
     2881 ctgtaaatca atggttcggg ttattaaatg ctccattatt ataatttttt atgtaaatat
     2941 attttttgca tttcgaaggt ttcagtctcc gatttgtatt ttgtctgtgt aatttcttca
     3001 attcgtcgga atcccagctt ttcatacaat ttcaccgccg gaatattttc aatccagacc
     3061 cgaataacgg catcggtgac tccgttcaac gtttcgtttt tcagatattc atgaataagt
     3121 gctgaggcta ttcccagtcc tcgtacatgt acatgaacca tcagttcggc tatataagcg
     3181 gtcgttttca ccggaatttc aggatgattc gcaaaaggaa attctgcatc ggatgtaaaa
     3241 ggaacaccca tcagagcacc aaccggtttt ccgtggagga aggcaaccgc tccgctgccg
     3301 actctttgaa attcatccaa cttttcggaa gcatcttcac gccttacata ctgagcaaat
     3361 tcaccggtag taaaagcgtg caagtataat tcgagcaact cttccttcca atcagagtag
     3421 taaagtggag ttagtttttc aatcgtatat ttgatttctg tgtggttcat cgtttcatta
     3481 tttaacaaaa ttaagcaaag gatgtttaca tcaaaaggtg attttaataa tatctcatct
     3541 tcacggcttc gacaattcat ttaatttctg taattttgtt cccgattttt aaaaaaactt
     3601 ttttttacta atgaacgaat gagaaaacaa aaaatccttt acctttcttt ttttatcagt
     3661 atcgtgctcg tatcctgcgg cggaaaaacc ggaaagggcg ctgaaagcga atccgttaca
     3721 gaagcagagc atcctcagtt tgtagattca acagcattcc atcaaaaatt aacccagctt
     3781 gccaacggtg acactaccgg actctggcct gtaaaaaacc aaccgattcc gctttacggc
     3841 gcattgctgc ccgaacacag aatagtggct tattacggca atttatattc aaaaaagatg
     3901 ggagcgctcg gcgagtacgc acccaaagaa atgtggtcca gattaaaagc cgaagtgaaa
     3961 gcctgggagg ctgctgaccc aacgactccg gtgattccgg cactccatta catcgccgtg
     4021 gtggctcagg gagatccggg caaagacggc aaataccggt ttcggatgcc tgagaaacag
     4081 attgattctg tatttaccat atctcgtatg caccccggta cgctggtatt tctggatatt
     4141 caggtggcgt tgagcaaaat tcaggatgaa ttgccactgc tggaaaaata cctgaaactg
     4201 ccgaacgttc acctgggaat tgacccggaa ttctccatga aagacggatc caaacccgga
     4261 aagcgaatcg gtacgtacga tgctaaagac gtgaattact gctcgcaata cctggcaaaa
     4321 ctggtaaaag agcacaatct gcctccgaaa gtgtttgtca ttcaccgttt tacacaaggc
     4381 atgataacca actataaaaa tatccggttg caccccgaag tacagatagt gattgatatg
     4441 gatggctggg gcgaaccgga actgaaaaaa ggtacgcacc gcaactacat tcacaaagaa
     4501 ccgatacaat tcaccggttt caaactcttc tataaaaacg acctgaaaaa atcgcctaac
     4561 cggatgatga cgccgcagga actgctcaaa ctccgtccac aaccgagtta cattcagtat
     4621 cagtaatcac ggtacttaac ggaaattcta tactcaaagc tcaaagggct gcttatcact
     4681 ggcagccttt ttttattttt tttggatgag gcacgcaaat atataagggc aagcagggct
     4741 tacagttttt tgtaagtgtc tttcagggaa taattccaat catttcagat tggaaaactt
     4801 acatgtaatc ctttattggg ttctgccagt aaattggtga tttcaaaatt cacgtcaccg
     4861 cctgtcgcac cgttagcaag tttcacagtg atggtatgcc tgccatgtgc atctatggtg
     4921 taatccctga aataaaccaa atcgggattg tgcgaagttt tatgcagata aaaaggtaag
     4981 tcggaattgt ttttcactgt tatcctgtaa tatttatccg tcttttcggt attcacaatt
     5041 tcgatggatt gatgaaatat ttccttcagg tatttttctt ctcctatcag cttgtcattg
     5101 agataaacca ctgtcctgcg ttcgatcagc gcttcatgga ttgcttcagg agttctggcg
     5161 cttgcaaaaa caagtgtcat cgtacggtgt tcaccttctt tcggtgtgag aggatcatga
     5221 acatcggtgt tgccgagcat ggtaagtttt ttctccatac accatcgctg agcttcgggg
     5281 taatattcag ttccgttcac cacttcaatt ccctgcatta gtccggcatt ataaagctcg
     5341 gtatgttcct taaaccaacg cgtggtatcg ggttgttgtg catcccatcc aggatgattc
     5401 caaaaaataa aagcattctg agctttggca gctttgaaag catccatata aaccgacaca
     5461 tcgagtgcat tgcaatcaga gagaaagagt gcattggaat gtcccggagg catcgaacgg
     5521 gttatttcag agccacgaat gagaatgata ttgtttgacc gtgctgctgc ttcagctatt
     5581 tcatatggtc gattgagaga tgctccgggc atgtcgttct tgtgaggacg gtattcaata
     5641 tgatcggtaa tggcaatggc atcgagacct tgtgaataag cttcgcttac acgaactgta
     5701 ggccatacca gaccgtcgga aaacaccgtg tgcatatgga agtcacattt gagggtttta
     5761 aatcccaata tgtcggggat ctgaatttca tttctgacct gtgaatatga caaaaccgaa
     5821 atg
//
LOCUS       DAORKP010000132         5693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2930_length_5693_cov_9.027315, whole genome shotgun sequence.
ACCESSION   DAORKP010000132 DAORKP010000000
VERSION     DAORKP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_5693_cov_9.027315"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1093
                     /locus_tag="PLN75_07580"
     CDS             23..1093
                     /locus_tag="PLN75_07580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRG04015.1"
                     /translation="MSVSVVILNWNGKKYLEEFLPFLLESVSDMPEAEIVVADNASTD
                     DSVAFLKKHYPTIKTDVFDQNYGFAGGYNRALEKIKSEYYVLLNSDVEVTKDWLKVLY
                     NYMQSNPRVAACQPKILSYHRRNYFEHAGAAGGYIDKYGYPYCRGRIFGEIEEDKGQY
                     DDIRDVFWASGACFFIRSEDYWNAGGLDEAFFAHMEEIDLCWRLKSRGRRIVCNPQSV
                     VYHIGGGTLKTEHPRKTYLNFRNNLLMLYKNLPHKSLKKTFDVRFYLDNLAAFHMLLQ
                     GKIQNLKAITDARTDFRNMQADFAEKRKENILKSVQSTFQEISQSSLLYEFYIKRRKK
                     FSDIPRNEIIVEFEEPQQEKEE"
     gene            complement(1116..2246)
                     /locus_tag="PLN75_07585"
     CDS             complement(1116..2246)
                     /locus_tag="PLN75_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRG04016.1"
                     /translation="MLTNIQTKDISELYSTPIVQQTAFWSVVKKKLGATTIAINFKSK
                     ESFIYHTPASQQDDIIVSDLLVILQKIDRDHSIAYVPYGPELEPAEEFQGMFLEELSE
                     SIRSFLPKNCIMIRYDLCWQSYWAKDSDHFDENGYWLGDPPTTTQELRFNFNTLNWNF
                     RKASYNILPSNTIYLNLNRETDEILASMKAKTRYNIGLALRKGVVVRSSDIDQIDVWY
                     DLYKQTAARNNIFLNDEKYFRAVLTTKINDSKSPAEVKLLVAEYNGKPLAAMFLIITG
                     HRGTYLYGASSAEHRNLMATYALQWEAIKISKSKGCTEYDMFGVSPGPDVNHPMYGLY
                     RFKTGFGGEIFHSLGCWDYPLNVEKYNIFTATELYSQGYHVR"
     gene            2418..4544
                     /locus_tag="PLN75_07590"
     CDS             2418..4544
                     /locus_tag="PLN75_07590"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRG04017.1"
                     /translation="MLIKYFTTILFSFISLALFASENLLVDSIMKQVMRAAETYSGVT
                     RSYEADVYMRTLVETKKKNFLYQYTYMIPNFVLHDRKNDQAIIESVSKLIFRAPNNYS
                     QDINYVTGTLTKKSDIAMLPFRFVSMDVYDESAHGEIFFLPLREKSSRYYTYSISYVE
                     HQDNRTIYSINFTPIYKNPQLISGYFLVEDNTWRVLGMYGEGNDLFVDFTFDIRMGDK
                     IANQFLPVKFNIVQVYSYLGNVVYNEYQADLDYKNIEFKTVETKKKNYNLGNSYRVRM
                     DSVPLNNDTVLWEQFRTSPLNDQEIELVETHNKRAMIAKAERMAKDSLSPQNDAALQL
                     MKNVAINTNYRFKSGDLRFSGLLNPSMIGYSTIDGLSYGQKMALAINLPREQRINVNL
                     FGGYIFGRKQLRYGIKSVWNYEPTKLGYLSLSAGRGNPSYSSRFLGLIKDSLLNYDEL
                     NLAYYIDYYFKLFNNIEIRNGLQLGLGIDYHIRQPNNKNQVSPVYNGFHQILTTQYNF
                     VPLISVSWTPEQYYVMDRNQKVYVRSDYPTFKIEFAQSINGVLGGKSSYNRVEFDVNQ
                     RIKFGLMKSLNYHAGFGIFSRQKNEYFNDFSYFAKSYFPETWGDGIGGGFSVLRYRYY
                     NSTDKYAQAHLMYESSTFLFTRLRSLSSGVARERLYLSQLYTPFMKSYTELGYGIGNR
                     FLNAAIFVGFHQLKYGSITAKAAFLL"
     gene            complement(4608..5558)
                     /locus_tag="PLN75_07595"
     CDS             complement(4608..5558)
                     /locus_tag="PLN75_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04018.1"
                     /translation="MNKTTSILIILTAFLLAGCSKNDDLEMDNIKMSTVLSIMVKDGK
                     AGMTTLVNDNYNVTTEYLIDNTKTDAQALQAFMPKGSFYRTTIDNYYLASTLYKNNEG
                     TIVEYKFPRIYSFEPGNQFIYFKNGERFAMDTLALGYFHSVDAAENEVFAGFFGTREI
                     YINGSYLLPAKAFYREGTGKITVLPMPDKNLNFNGVSCVHRSGNNIYVGGLMDFPMYW
                     KNKEIVQLNEKYGEVNQIFTVGNDVYAVGFYNKANSKTTGHTACYWKNGLLTELEDNA
                     VGYSIFIDGNDIYVGGAVGRFDAEYKACYWKNGKRVLLPV"
BASE COUNT         1676 a         1182 c         1182 g         1653 t
ORIGIN      
        1 tttatcatag ttaaataaat atatgtcggt atcagtagta atacttaact ggaacgggaa
       61 aaaatacctc gaggaatttc tccctttctt gcttgaaagt gtaagcgata tgcctgaagc
      121 tgagattgtg gtggctgata atgcttctac cgacgattcg gtagcgtttc tcaaaaaaca
      181 ctatccaact atcaaaacag atgtttttga tcagaattac ggttttgcag gcggttacaa
      241 ccgcgctttg gagaaaatca aaagcgaata ttacgtgttg ctcaattcgg atgtggaagt
      301 gacgaaagac tggctgaaag tcctctacaa ttacatgcaa agcaatccgc gtgtggcagc
      361 ctgccagccc aaaatccttt cgtaccaccg ccgcaactat tttgaacatg ccggagctgc
      421 aggcggatat atcgacaagt atggttatcc gtactgccgc ggacgaattt ttggagaaat
      481 agaagaggat aaagggcaat acgacgatat acgcgatgtg ttctgggcat cgggagcctg
      541 tttttttatt cgttcggaag actactggaa tgcaggcggg ctcgacgaag cgttttttgc
      601 tcacatggaa gaaatagacc tttgctggag gctcaaaagc aggggaaggc gcatcgtatg
      661 caatccacaa agcgtggttt atcacattgg aggaggcaca ctgaagaccg aacatccacg
      721 aaaaacatac ctgaatttca gaaataatct cctgatgctt tacaaaaacc tccctcacaa
      781 gagtttgaaa aaaacctttg atgtacggtt ttacctggat aatttagccg cttttcatat
      841 gcttcttcag ggtaaaatac aaaacctgaa agctattacc gatgcccgta ccgacttcag
      901 aaacatgcag gctgattttg ccgaaaaacg aaaagagaat atcctgaaat ctgttcagtc
      961 aacttttcag gaaatttcgc agagcagtct gttgtatgaa ttttacatca aacgccgtaa
     1021 aaaattcagc gatattcccc gtaatgaaat tatcgttgaa tttgaagaac cgcaacagga
     1081 aaaagaagaa taaccacatt tcccggaatg cggctctaac gaacgtgata gccctggctg
     1141 taaagttcgg tggctgtaaa aatattgtat ttttccacat tcagcggata atcccagcat
     1201 ccgaggctat ggaatatttc acctccaaag ccggttttga aacgatacag gccatacatg
     1261 gggtggttta cgtccggacc gggtgaaact ccgaacatat cgtattcggt gcaacctttt
     1321 gacttcgaaa ttttgatagc ttcccactgc aaagcatagg ttgccatcag atttcggtgc
     1381 tcagccgacg aagcaccata gaggtaagta cccctgtgtc ccgtaatgat taaaaacatg
     1441 gctgccaatg gcttaccatt gtattctgcc accagcagtt tgacttctgc cggagatttc
     1501 gaatcgttta ttttggtggt gagcacagcc cgaaaatact tttcatcatt cagaaatatg
     1561 ttgtttctgg ctgcagtttg tttatacaga tcataccata cgtcaatctg atcgatatct
     1621 gagctcctta ccacaactcc cttgcgcaat gccagtccta tgttatacct ggtttttgct
     1681 ttcatggaag cgagtatttc atccgtttca cgattcagat taagataaat ggtattcgaa
     1741 ggcagaatgt tgtaactcgc tttccggaag ttccagttaa gagtattgaa gttgaatctg
     1801 agttcctgag tagtggttgg cggatcgccc agccagtaac cgttttcatc aaaatgatcg
     1861 ctgtctttag cccagtaaga ttgccagcac aaatcgtacc ggatcatgat gcagtttttg
     1921 ggcagaaatg agcggatgct ttccgacaat tcttcaagaa acatgccctg aaattcttct
     1981 gccggctcca gttcgggccc gtaaggcaca taagctatgg aatgatcgcg gtcaattttt
     2041 tgcaatatga ccagcaaatc agatacaatt atatcatcct gctgagatgc aggagtatga
     2101 tagataaacg attcctttga tttgaagttg atggcaatgg tagttgcacc cagcttcttt
     2161 ttaactaccg accagaatgc cgtttgctgc acgatgggcg tggaatataa ttcgcttatg
     2221 tctttggtct gaatgttcgt cagcataatt ttttttctga aaataaatga ttcaactcct
     2281 gcaaaggtaa ggacaaacat cggggaataa aaatatctca tacaacctga aaaattaaaa
     2341 gatttgtttc ataaggtgtt agatataatt tattcgaaaa aaagcgttaa gtttgtattc
     2401 tgtttttatt tttcccgatg cttataaaat atttcactac catattattc tcattcattt
     2461 ctttagctct ctttgcttcc gaaaatctgt tggttgacag tatcatgaaa caggtgatga
     2521 gagctgcaga aacctacagc ggtgtgacca gatcgtacga ggctgatgtg tatatgcgta
     2581 cgctggtaga aacaaaaaag aagaattttc tgtaccagta cacctatatg ataccgaatt
     2641 ttgtactgca cgaccgcaaa aacgaccagg ccattatcga gtcggtaagc aaacttatct
     2701 tcagggcacc aaacaattac tcccaggata tcaactatgt gaccggcaca ttgaccaaaa
     2761 aaagtgatat cgccatgctg cctttccggt ttgtgagtat ggatgtgtat gatgaaagtg
     2821 ctcacggtga gatatttttt ctcccgcttc gtgaaaaatc gtccagatat tacacctatt
     2881 ctatctctta cgtcgaacat caggacaacc gcaccattta ttccatcaat tttacgccca
     2941 tatataagaa tccacaactc ataagtggtt attttctggt tgaagacaat acctggcgcg
     3001 tacttggcat gtatggcgaa ggtaatgacc tgttcgttga ttttactttt gatatccgga
     3061 tgggcgacaa aattgccaat cagtttcttc cggtaaaatt taatattgta caggtttaca
     3121 gctatctggg caatgtggtg tacaacgaat atcaggccga tctggattat aagaacatcg
     3181 aatttaaaac cgttgaaacc aaaaagaaaa actacaacct gggcaacagc taccgcgttc
     3241 gtatggattc ggtgccgttg aacaacgaca cggtgctttg ggaacaattc cgcacctcac
     3301 cgctgaatga tcaggaaatt gaacttgtgg aaacgcataa taaaagggca atgattgcca
     3361 aagccgaacg gatggctaaa gactcgcttt cgcctcaaaa tgatgcagct ttacagttga
     3421 tgaagaatgt ggctataaat accaactacc ggttcaaatc gggcgactta cgctttagcg
     3481 gactgctcaa tccatccatg ataggctatt ccaccatcga tggcttatcg tacggacaaa
     3541 aaatggcgct ggccatcaat ctgccccgtg aacagcgaat caacgtgaat cttttcggag
     3601 gatatatttt cggcagaaaa caactccggt atggtattaa aagcgtatgg aactacgaac
     3661 ccaccaaact tgggtacctt tcactgtcgg ccggaagagg aaatccttcc tacagttccc
     3721 gctttctcgg tttgataaaa gacagtctgc tcaattatga cgaactgaat ctggcttatt
     3781 acatcgacta ttatttcaag cttttcaaca atattgaaat cagaaatgga ttacagctcg
     3841 gcctgggtat cgactatcat atccgtcaac caaataataa aaatcaggtc agtcctgtct
     3901 ataacggttt tcatcaaata ctcactactc agtacaattt tgtgcccctg attagtgtaa
     3961 gctggacacc cgaacaatat tatgtgatgg accgaaatca aaaagtgtat gtgaggtcgg
     4021 attatcccac tttcaaaata gaatttgctc aaagtatcaa cggagtgttg ggaggaaaat
     4081 cgtcctacaa cagagtggaa tttgatgtaa atcagcggat taaatttgga ttgatgaaga
     4141 gtctgaacta tcatgccggt tttggaatat tcagtcggca gaaaaatgag tatttcaacg
     4201 atttcagcta ttttgccaag tcttattttc ccgaaacgtg gggcgatgga ataggaggcg
     4261 gattcagtgt actccggtat cgttattata actcaaccga taaatatgcc caggcacacc
     4321 tgatgtatga atcatcgact ttccttttta cacgattgcg cagtctgtcg agcggagtag
     4381 cccgtgagag gttatatttg agtcagctat acaccccgtt catgaaatca tataccgaac
     4441 tgggttacgg aatcgggaac cggtttctga atgcagcaat atttgtcggt tttcatcagc
     4501 tgaaatacgg aagtatcacg gctaaagctg cattcttatt gtaaacagaa aaagagccgg
     4561 agtaggccga ccctgtaaaa ttaaatggtg agaatagaaa aatcctgtta aaccggcagc
     4621 agcactcttt ttccgttttt ccagtagcag gctttgtatt cggcatcgaa tctgcccacc
     4681 gctccgccta cataaatatc attcccatca atgaaaatgc tgtagcctac agcgttgtct
     4741 tccagttcgg tcaacaatcc gtttttccag tagcaggctg tgtgtccggt ggtttttgaa
     4801 ttcgccttgt tgtaaaaacc aactgcataa acatcattcc cgacagtgaa aatctgattt
     4861 acttcgccgt atttttcatt taactgaaca atttccttgt ttttccagta catcggaaaa
     4921 tccatcaaac cgcctacgta aatgttatta ccggagcgat gtacgcaact tactccgttg
     4981 aaattcaggt ttttatctgg cataggcaga acggtaattt ttccggtacc ttcgcgatag
     5041 aatgctttgg caggcaaaag ataagagccg ttgatataaa tctctcttgt accgaaaaaa
     5101 ccggcaaaaa cctcattctc agcagcgtca accgaatgaa agtatcccag tgccagcgtg
     5161 tccatggcaa agcgttcgcc atttttgaaa taaataaatt gatttccggg ttcgaatgaa
     5221 tatatgcggg gaaacttata ttcaacaatt gtaccttcgt tatttttgta aagcgtagaa
     5281 gcaaggtaat aattatcaat ggttgtacgg taaaaactcc ctttcggcat aaaagcctgc
     5341 agagcctgag catcggtttt tgtgttgtca atcaggtatt ctgtagtcac attgtaattg
     5401 tcgttcacga gtgtggtcat cccggctttt ccgtctttca ccataatgga aagcacagta
     5461 ctcattttaa tattatccat ttcaaggtcg tcattcttac tgcaacctgc aagtaaaaat
     5521 gctgtcagga tgattaatat agacgttgtt ttattcataa ggcttttttt tataatagtt
     5581 ggttttccaa ttctgcttta ttatatgatg aaaataagca tcaaaacgct gcatacaatg
     5641 ttgttaaata atcttaaatg agcatttttt ttcctttctt ctccttgctg att
//
LOCUS       DAORKP010000133         5676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2947_length_5676_cov_6.913716, whole genome shotgun sequence.
ACCESSION   DAORKP010000133 DAORKP010000000
VERSION     DAORKP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_5676_cov_6.913716"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..927)
                     /locus_tag="PLN75_07600"
     CDS             complement(7..927)
                     /locus_tag="PLN75_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRG04019.1"
                     /translation="MAIPTTRTKENATISIDSEKCTGCGECVSVCKDFNFYIENKKAK
                     ISGSPIFGCIACGHCMAVCPAGAIEISGRELSKDDLFDLPLRKDAANYEQLYALFARR
                     RSIREFQDKDIEREIIQKILDAAVTSPMGLPPSDVNVLILDSREKTRRFAVDFCAFLD
                     NMKWFVSGWFLALMRPFWGKTNDEMFKGFVKPLFNIYIRNMQAGINLVNYDAPLAMYF
                     YGSPYTDPADPIVAATTAMYAAESLGLGTCMLGAIHPLIQSGRKAKIFREKHGIKYTS
                     REGLFVIFGYPAVKYQKGIKRTFASTDVMN"
     gene            complement(981..1613)
                     /locus_tag="PLN75_07605"
     CDS             complement(981..1613)
                     /locus_tag="PLN75_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRG04020.1"
                     /translation="MKITVFGASGKTGRLLIEEALASGHEVVAYARTKESINTVHPKL
                     KVVVGQLSNKEKLKSAISGSDACISTLGGSSMTKHSTEIMEGIANIIAIMEELNVKRF
                     IYMSSIGAGKSRNFMPQPVRFFIADLMLRVPLADHTANENRIMNSKLEWTIIRPGGLT
                     DGAKSDTLKHGSEFTKLTGNLRISRSGVAAFILSQLTDTNYLNKSVWLYE"
     gene            1813..3081
                     /locus_tag="PLN75_07610"
     CDS             1813..3081
                     /locus_tag="PLN75_07610"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRG04021.1"
                     /translation="MEIKPKDKIVIVGGGFAGMRLVKELKNQPVSVTLIDKQNYHSFQ
                     PLFYQVASARLEPASISFPFRKVFQNYRNFEFRMAEVKSADVKNNLLMTSNGIVHYDH
                     LIVSTGCTTNFFGNEEIRKNSLAMKTMQEAIEIRNRILLDFETYLTASFEEQEELMNI
                     VIVGAGATGVELAGAFAELKNDILPKDYPNVDFSGLKIILLEGSPFTLGVMSDESKKM
                     SRKYLEDLGVEIKTGVILNSYDGKTAKLNTGETIPCRNLIWSAGVTGNIIEGIAGKEN
                     IVRNRYIVDRFNLVKGTKNVYALGDVAYMKTELYPNGHPQVANVAITQAKNLAENMKK
                     SLQGKKPAEYEYKNKGSMATIGKHKAVVDLNFWKFQGFFAWLIWMFLHLMLILSVRNK
                     LIIFINWAWSYFTKDTSLRLIINPEEEDIG"
     gene            3411..4619
                     /locus_tag="PLN75_07615"
     CDS             3411..4619
                     /locus_tag="PLN75_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04022.1"
                     /translation="MKRPVLFLLLILSCCINSRSRAAVSDADSLTRPKMPLLAWFSIP
                     EAYTDSNRFGELAETGINQSLTFYSSADAAMKALDMAEKANVKVIVACPELETDTEKT
                     VRRFMNHPALAGYYLRDEPDVSLFQKLNAWVRKIEAVDSTHYCYINLFPNFVSPNSLG
                     TPQYSDYLTRFLEEVPVRYLSFDNYPIVEKKLRPLWYQNLEIIANSSRQFNLPFRAFA
                     LTTAHGKYPIPGLAELRLQVFSNLAYGAQGIQYFTYWTPPPVEWDFHHGPINADGQRT
                     NTYELIRTMNAEINALSGVFLGAKLIAVYHTGTFIPKGTNRLKKLPLPFLRIKTGNPG
                     AVVSFLQNKDKKYLVIVNRSMLESTFVRIEFNSDSVKQVMKDGKTNSLPGGFNRFEVE
                     PGDVLILNYP"
     gene            5084..>5676
                     /locus_tag="PLN75_07620"
     CDS             5084..>5676
                     /locus_tag="PLN75_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRG04023.1"
                     /translation="MMEKLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEGLGVDVIE
                     AGFPISSPGDFKSVVEISKAVTWPTICALTRGVEKDIEVAAEALQYAKRGRIHTGIGT
                     SEQHIKYKFNSTQDEILERAVAATKYAKKFVEDVEFYCEDAGRTDNVYLARVVEAVIK
                     AGATVVNIPDTTGYCLPWEYGEKIKFLFENVKNIHQAV"
BASE COUNT         1737 a         1172 c         1163 g         1604 t
ORIGIN      
        1 aaataattaa ttcatcacat ctgtagaggc aaacgttcgt ttgattcctt tttgatattt
       61 cacagccgga tacccgaata tgacaaagag tccttcgcgg ctggtgtatt ttatcccgtg
      121 cttttccctg aagattttag cttttctgcc gctttgaatg agcggatgaa tggcacccag
      181 catgcaggtt cccaatccca gagattcggc ggcgtacatg gctgtagtgg cagctactat
      241 cggatcggcc ggatctgtat agggtgaacc gtagaaatac atggctaaag gggcatcgta
      301 gttcaccagg ttgattccgg cctgcatgtt ccggatataa atattgaaaa gaggctttac
      361 gaaacctttg aacatttcgt cgttcgtttt gccccagaat ggccgcataa gcgccagaaa
      421 ccaaccagaa acaaaccatt tcatgttgtc gagaaatgca caaaaatcaa ctgcaaatct
      481 ccttgttttt tctctgctgt cgagtattag cacatttaca tccgaagggg gtagtcccat
      541 cggcgaagtg acggcagcat cgagaatttt ttggatgatt tctcgctcaa tatctttatc
      601 ctgaaattct ctaatgcttc ttcgtcgggc gaacaaggca tacaactgct cgtagttagc
      661 agcatccttt cggagcggca agtcaaacaa atcgtctttg gataattccc tgccggaaat
      721 ttctatggct cctgccggac atacagccat gcaatgtccg caggcaatgc atccgaatat
      781 cggtgaaccg gagatttttg cctttttatt ttcgatgtaa aaattgaaat ctttacatac
      841 cgaaacacat tcaccacaac ccgtgcattt ttcggagtca atgctgattg tagcattttc
      901 ttttgttctt gtagtcggaa ttgccatttt atttttttaa aaaaaaatcc tgatattagc
      961 tgcctcgttg ctgaagtgat ttattcatac aaccagacgc ttttattcaa ataattagtg
     1021 tcggtaagtt gagagagaat aaacgctgcc actcctgaac gtgaaatcct cagatttccg
     1081 gttagttttg tgaattcgga accgtgtttc aacgtgtcag attttgcacc gtcggttaaa
     1141 ccaccggggc gtatgatcgt ccattcgagt ttgctgttca tgatacggtt ttcgttggct
     1201 gtgtgatcgg ccaaaggtac acgcagcatc aggtcggcaa taaaaaaccg aactggctga
     1261 ggcataaaat tccggctttt tccggcaccg atactcgaca tataaatgaa gcgtttcacg
     1321 ttcaactctt ccattatagc gataatattg gcaattcctt ccattatttc ggtagagtgt
     1381 ttcgtcatgg acgatccgcc caaagtagag atgcaggcat ccgacccgct aattgctgat
     1441 ttaagttttt ccttgttgct gagctgacct acaactactt ttaatttagg atgaaccgtg
     1501 ttgatcgatt ctttcgtgcg ggcataggca actacttcat ggccggatgc taatgcttcc
     1561 tctatcaata accggcctgt ttttccactg gctccaaata ctgttatttt catgtttttt
     1621 attttgtaaa atgtataatt caggaaagaa acgctctgaa actttttgca gggagcactc
     1681 taagaatggt tttctcccct ggttttcaaa tcagattttc cgtttccaca aaattacttt
     1741 tttttctcca tgctataact tttcatcagc aaatttgttt tagttttaat ttttcaatta
     1801 aaagaatcaa gtatggaaat aaaaccaaaa gacaaaatag tcattgtagg aggtggcttt
     1861 gccggcatgc gactggttaa agaactgaaa aaccaaccgg taagtgtcac cctgattgat
     1921 aaacaaaact atcactcgtt tcagccacta ttttatcagg tagccagcgc ccgactcgaa
     1981 ccggcaagca tttcatttcc tttccgaaaa gtatttcaga attaccggaa ttttgaattt
     2041 cggatggcag aagtcaaaag tgccgatgta aaaaataacc tgttgatgac cagcaacggt
     2101 attgtgcatt atgatcattt aatagtgagc acaggctgca ctaccaattt tttcggcaac
     2161 gaagagatcc gtaaaaactc actcgccatg aaaaccatgc aggaagccat tgaaatcagg
     2221 aaccggattt tgcttgattt tgaaacatac ctcactgcga gtttcgaaga acaggaggaa
     2281 ctgatgaata ttgtcattgt gggagcagga gctaccggag tggaactggc cggagcattt
     2341 gcggaactca aaaacgatat tctgcccaaa gactatccga atgtcgattt ttcggggctc
     2401 aaaatcatac ttcttgaagg gagtccattt acgctgggtg taatgagcga cgaatcgaaa
     2461 aaaatgtcga gaaaatatct cgaagattta ggcgtagaga tcaaaacggg cgtgatactc
     2521 aattcatacg atggtaaaac agcgaaatta aataccggcg aaaccattcc atgccgcaat
     2581 cttatctggt cggcgggcgt tacgggaaac atcatcgaag gtatagccgg gaaagaaaac
     2641 atcgtcagaa accgctatat agttgacaga tttaaccttg tgaagggcac aaaaaatgtg
     2701 tatgctttag gcgatgtagc ctacatgaaa actgagttgt atcccaacgg gcatccgcag
     2761 gtagccaatg tggccatcac ccaggccaaa aatctggccg aaaacatgaa gaaatccctg
     2821 cagggcaaaa aaccagccga atacgaatac aaaaataaag gctccatggc cactatcggt
     2881 aaacacaaag ccgtagtgga tctgaatttc tggaaatttc agggattttt tgcatggctc
     2941 atctggatgt ttcttcacct gatgctcata ttaagcgtaa gaaataaact gataatattc
     3001 atcaactggg cctggagcta tttcaccaaa gacacttcgc tcagattgat catcaatccc
     3061 gaagaagaag atataggttg aaaaaaaatg aaacagacat tagttatcaa acctgaaata
     3121 tagacctctc tcaccattca ctcgggaaat aattttatcg gtcatactta aatttgcaat
     3181 atcacaaaca aaattagttc attcttcatt ctgccagaaa tgaatactga gacaaattta
     3241 ttcaaaaaaa agttaggtct gttaaaattt gcagaacaat catgcaatgt ttgcaaacag
     3301 gcttacaact atctttttta attccaccat tacatgaata tgaattagtc agttaaaatt
     3361 tgcttttgtt ggaaaaggtt gagtgttgaa aatccaaata atatcccaca atgaaaagac
     3421 ctgttttgtt tttattactg attttaagct gttgtataaa tagccggagt cgtgcagccg
     3481 taagcgatgc agattcactg acaagaccta agatgcctct gttagcctgg tttagcattc
     3541 ctgaagctta cactgactcc aatcggttcg gcgaactggc agaaacggga attaatcagt
     3601 ctttaacatt ctacagctct gcagatgcag ctatgaaagc cctggatatg gccgaaaagg
     3661 cgaatgtgaa agtcattgtt gcctgccccg agttggaaac tgatacagaa aaaacagtca
     3721 gacgttttat gaatcatccg gctctggcag gatattactt acgggatgaa ccggatgttt
     3781 ccctgtttca gaaactgaac gcatgggtaa gaaaaattga ggcggtggac agtacgcatt
     3841 attgctacat caatttgttt ccgaactttg tgtcacccaa cagccttgga acgccgcaat
     3901 acagcgacta cctaacccgg tttctggaag aagttcccgt gcggtatctt tctttcgaca
     3961 attatccaat agtagaaaaa aaactgcgcc cgctgtggta tcaaaatctg gaaattattg
     4021 ccaattcctc ccggcagttc aacttacctt tccgggcatt tgcgctgact actgcccatg
     4081 gtaaataccc catccccggt ttggctgaat tgcggctgca ggtattcagc aacctggcat
     4141 acggagctca gggtatccag tactttacat actggactcc cccacccgtg gaatgggatt
     4201 ttcatcatgg ccccatcaac gccgatgggc aacgcacaaa tacgtacgaa ctaatcagga
     4261 cgatgaatgc agaaatcaat gctctttcgg gcgtgttttt gggtgcaaaa cttattgcag
     4321 tataccacac aggaacattt atacccaaag gcaccaatcg gctaaaaaag ctacccttac
     4381 catttctgag aataaaaaca ggcaatcccg gagctgttgt gtcttttctt caaaacaaag
     4441 acaaaaaata cctggtaatc gtaaaccgga gtatgctgga gtcaacattt gttcgtattg
     4501 aatttaattc cgactccgta aaacaggtta tgaaagatgg aaaaacaaac tctttgccag
     4561 gcggattcaa tcgttttgaa gtagaacccg gcgatgtttt aattctgaat tatccttaaa
     4621 attcagtgaa agtactcact attaaatgtc agggtccgtt tatgaaactt caaactgttt
     4681 tcacaaaata acgataaatt cattacaaat cagaatgaaa attggtttta tgttacaaat
     4741 tcaatttttt ttatgctttt ttgtttgata tttgaaaatt ttgatttacc tttgcatcgt
     4801 tttataaaaa aagcacaaca atgtcaacct ctttcaacca cattattctc ctcggtctca
     4861 ttattctctt acaaaataag acgaagtgag tttggtatgt gtttgattca aaataatgaa
     4921 aaaatataaa acctttctca cacaagagaa aggttttttt taataaaaga aatgacccct
     4981 aactactaaa ggggaatata gaaaaaacaa ttatagaaat tattataaac ttaatatgcc
     5041 cttaacaatc actttgaata gataaaaaag ggacgggaga tttattatgg aaaaactttt
     5101 tatttttgac acaaccctgc gcgatggcga gcaggttccc ggttgtcagt tgaatacagt
     5161 agagaaaatt caggtagcca aagcattgga aggacttggc gtggacgtga ttgaagccgg
     5221 tttcccgatt tcaagtccgg gtgatttcaa atcggttgta gaaatttcta aagccgtaac
     5281 ctggccaact atctgcgcac tcacacgcgg cgtggagaaa gacattgaag tggcagccga
     5341 agcgcttcaa tatgccaagc gaggacgaat ccacactgga ataggtactt ccgagcaaca
     5401 tataaagtat aaatttaact ccactcagga tgaaattctg gaacgcgcag ttgcagctac
     5461 caaatatgct aaaaaattcg tggaagacgt ggaattttat tgcgaagatg ccggacggac
     5521 agacaatgtg tatctggcac gcgtagtaga agccgtgatt aaagctggtg caaccgtggt
     5581 aaacatcccg gacacaaccg gatattgcct gccatgggaa tacggtgaaa aaatcaaatt
     5641 cctttttgaa aatgtgaaaa atatccatca agcggt
//
LOCUS       DAORKP010000134         5650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_2984_length_5650_cov_9.596962, whole genome shotgun sequence.
ACCESSION   DAORKP010000134 DAORKP010000000
VERSION     DAORKP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_5650_cov_9.596962"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLN75_07625"
     CDS             <1..663
                     /locus_tag="PLN75_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRG04024.1"
                     /translation="ETGVRIHSLNGQKEELQKKLGEIAEKFRKDAEIKARNVRVVEEI
                     TLQEKVCLKWTTLMSILGGSQDAFNTYVQRLTLRNLIDLANLHLYKLNPRYSLQLNPQ
                     YKSGEELNFKLVDHYQAGETRPVDTMSGGEKFLISLSLALGLSDLASQNVTIGSLFID
                     EGFGTLDSNTLETVISTLETLKSQGKMIGIISHVDSLKERIPVQIQVLKKSNGVSTVT
                     IS"
     gene            complement(785..3565)
                     /locus_tag="PLN75_07630"
     CDS             complement(785..3565)
                     /locus_tag="PLN75_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRG04025.1"
                     /translation="MAKEDELNEMEDQDLPDESPENAEQASDTDDAGETHSNYIIPKI
                     KDDESVKHHLSGMYQTWFLDYASYVILERAVPEIHDGLKPVQRRILHSMKRLDDGRYN
                     KVANIVGHTMQFHPHGDASIGDALVQLGQKDLLIDTQGNWGNILTGDGAAAPRYIEAR
                     LSKFALETVFNPKITEWKLTYDGRNKEPISLPIKFPLLLAQGVEGIAVGLNSKILPHN
                     FNELIDASVAHLRGEDFVIYPDFQTGGSIDVSKYNDGERGGSVKIRAKIGKLDNKTLI
                     ISEIPFGTDTSKLIESIIKASDKGKIKIRKVDDNTAQNVEILVHLAPGASSDKTIDAL
                     YAFTGCEASISPNCCVIHDKKPRFIGVSELLRISTDHTKELLKLELEIQRDELLEQLF
                     FVSLEKIFIENRIYKDREFEESETQDAVIKHIDSRLEPFKKDFIREVTREDILKLLDI
                     KMIRITKFDTKKADDTIIGIQKKIRELEYNLENLVEFTIFWFQNLKEKYGKNYPRMTE
                     IRNFDTIVAAKVVEANEKLYVNHEDGFIGTGLKKDEFVCNCSDMDEIIVFFKDGKYKV
                     VKVAEKLFVGKNILYLNVFKKNDKRTIYNVVYRHGKNGPFYIKRFAVTGITRDKEYDI
                     SQGKEGSRVFYFSANPNAEAEVIRVTLKPKPRLKNLVIEKDFSEIAIKGRQSMGNILS
                     KNDVHRITLKHKGGSTLGGREVWFDRDVLRLNYDKRGDYLGEFLSEDLILVITTKGEF
                     RTTNFDLSNHFETEILTIKKFDPNKVWTVALYDAEQKFYYLKRFQLEPSAKLQSFLGE
                     NPESKLMLISGKTYPRIEVVFGRGDAFREALVVDAEEFIGVKSFKAKGKRLTTFEVES
                     INELEPIRFKPEKETEEKNEADETDEINADETSDEPETENENDESSETNSDEPIDQQR
                     LIDDITGQMTLF"
     gene            3755..4345
                     /locus_tag="PLN75_07635"
     CDS             3755..4345
                     /locus_tag="PLN75_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04026.1"
                     /translation="MKPKLFILFAIALLSVFTKAQTAEKGKIGLTYSFNGGTTVMQPA
                     LEGSASHDVTDATAIGIIYLKPLNNWLELETGITYSLCKITTTSAPTPEVNTYKSNLS
                     LIDIPVGLRAVFLKYFFVNGALLLDAGLMNNSSVSSQTGIGMMAGVGIKYDFRFGGSL
                     FFNPYGKIHSLVPFGGWKHHDRILESGWKLGITCAL"
     gene            complement(4419..4622)
                     /locus_tag="PLN75_07640"
     CDS             complement(4419..4622)
                     /locus_tag="PLN75_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04027.1"
                     /translation="MTYVTGSFKSAVTGFASENNIEFAWQPRFREYIICSQHEPERIY
                     EFIQNNVTAWNNDHFNPPTGADL"
     gene            4678..5256
                     /locus_tag="PLN75_07645"
     CDS             4678..5256
                     /locus_tag="PLN75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRG04028.1"
                     /translation="MISMGNKKELSPEQRDEIISTLKDRFEKNMNRHKGLEWDGLLAR
                     LNSGTRDEKLWSLREMERTGGEPDAVGYDEKTGEYIFYDCSAESPTGRRSLCYDREAL
                     ESRKTYKPENNAIDMAADMGIELLTEEQYRELQKLGNFELKTSSWVKTPAEIRKLGGA
                     LFCDRRYDRVFVYHNGAESYYAVRGFRGSLRV"
BASE COUNT         1470 a         1297 c         1263 g         1620 t
ORIGIN      
        1 gaaaccggtg tacggataca ttcactcaac gggcaaaaag aagaactgca aaagaaactg
       61 ggcgaaatag ccgagaaatt cagaaaagac gccgaaataa aggcgcggaa tgtacgcgtg
      121 gtggaagaga taacccttca ggaaaaggtt tgcctcaaat ggacaaccct gatgagcatt
      181 ctgggtggtt cgcaggatgc atttaacacc tacgtgcagc ggcttacgct ccgaaacctc
      241 attgaccttg ccaacctgca cctctacaaa ctcaatccgc gatattcact ccagctcaac
      301 ccacagtaca aatcgggcga agaactcaat ttcaaactcg tggatcatta tcaggcagga
      361 gaaacccgac cggtggacac catgagcggg ggcgaaaaat tccttatcag tctttcgctg
      421 gcgctcgggc tgtcggatct ggccagccaa aacgtgacca tcggctccct ttttatcgac
      481 gaaggtttcg gtacgctcga cagcaacacg ctcgaaaccg tgatttcgac cctcgaaacc
      541 ctcaagtcgc agggaaaaat gattggcatc atctcgcatg tggacagtct gaaggaacgt
      601 attcccgttc agattcaggt gctgaaaaag agtaacggag tgagcacggt taccataagc
      661 tgatattgtt tgccttattg tcagtttcgg cagctgtcaa acaagacata aaaaaaacca
      721 cctgatatca acgcaatata tcaggtggct ttattttaat aaatttttcc tctgcgtttt
      781 tttttcagaa aagcgtcatc tggcctgtta tatcgtctat caggcgctgc tggtctatgg
      841 gttcatcgct gtttgtttcg gacgattcat cattttcatt ttctgtttcc ggttcatcag
      901 aggtctcatc ggcatttatt tcatccgttt catcagcctc gtttttttct tccgtttcct
      961 tttcgggttt gaaacggata ggctccagtt cgttgatgga ttccacctcg aaagtggtca
     1021 gccgttttcc tttggctttg aagcttttca caccgataaa ttcttcggca tccacaacca
     1081 gagcctctct gaaagcatct cctctgccga aaaccacctc gatgcgcgga tacgttttgc
     1141 ccgaaatgag catcagtttt gattcgggat tttcgcccag gaaactctgg agttttgccg
     1201 aaggctccag ctgaaaacgt ttcaggtagt agaatttttg ctcagcatcg tacagagcta
     1261 cagtccacac tttgttcgga tcgaattttt ttattgtcag aatttcagtc tcaaagtgat
     1321 tgcttaaatc aaaattggta gttcggaatt cgcctttggt tgtgatgacc agaatcaaat
     1381 cctcacttaa aaattcaccc aggtaatcgc cccgtttatc ataattcaac cgcaacacat
     1441 cgcggtcgaa ccacacctcg cggccgccca gcgtcgaccc gcccttgtgt ttgagcgtga
     1501 tgcggtgcac atcatttttc gaaaggatat tacccatgga ctgacgcccc ttgatggcaa
     1561 tctcgctgaa atctttctca atcaccaggt ttttgaggcg tggttttggt ttcagcgtta
     1621 cgcgtatcac ttctgcttcg gcattggggt tggcgctgaa gtaaaacacc cgggagccct
     1681 cttttccctg cgaaatgtcg tattccttgt cgcgggtaat tcctgttacg gcaaaacgtt
     1741 tgatgtagaa aggaccattt tttccgtgcc ggtacaccac gttgtagatg gttcgtttat
     1801 cgtttttctt aaatacgttc agataaagga tgttttttcc cacgaaaagt ttttcggcca
     1861 ctttcaccac cttgtatttt ccgtccttga aaaatacgat gatttcgtcc atatccgagc
     1921 agttgcatac gaattcgtcc tttttcaatc ccgtccctat aaagccatcc tcgtgattca
     1981 catacaattt ttcgttggct tccaccactt tagcagctac aatggtatca aagttccgga
     2041 tttcggtcat gcgcgggtag tttttcccgt atttttcttt caggttctgg aaccagaaaa
     2101 tggtaaattc caccaggttt tcgaggttat actccagttc cctgattttt ttctgaatgc
     2161 cgatgatggt gtcgtccgct ttcttagtgt cgaatttggt gatgcgtatc attttgatat
     2221 ccagcagttt gagaatatcc tcgcgggtca cctcacggat aaagtccttc ttgaacggtt
     2281 cgaggcgact gtcaatgtgt tttattacgg catcctgcgt ttcgctttct tcaaattccc
     2341 ggtctttgta aatccggttt tctataaaaa tcttctcgag cgaaacgaaa aagagttgct
     2401 cgagcagttc atcgcgctga atctcgagtt cgagtttcag cagttctttg gtatggtcgg
     2461 tactgatgcg gagcagttcg ctcactccga taaagcgcgg tttcttgtcg tggatgacgc
     2521 agcagttggg cgaaatgctg gcttcgcagc ctgtaaaggc atagagggca tctattgttt
     2581 tgtccgacga agcgccgggt gccaggtgaa ccagtatttc cacgttttgc gccgtgttat
     2641 cgtccacttt gcggattttt attttcccct tatcgctcgc tttgataatg ctttcgatga
     2701 gtttggaagt atccgttccg aacggaattt cactgattat gagtgtttta ttatcaagtt
     2761 taccgatttt agctcttatt ttcaccgagc cgccccgttc gccatcgttg tatttgctta
     2821 catcaatgct tccgccggtc tgaaagtcgg ggtaaattac aaaatcctct ccgcgaaggt
     2881 gagcaacgct tgcatcaatc aattcgttga aattgtgcgg cagaatcttg gaattgagcc
     2941 ccacggctat cccctccaca ccctgagcga gcaacagcgg aaatttgatg ggcagcgaaa
     3001 tcggttcttt gttgcgcccg tcgtaggtga gtttccattc ggttattttc gggttgaaaa
     3061 cggtttcgag cgcaaattta gaaagccgtg cctcgatata acgtggcgca gccgcgccat
     3121 cgccggtaag gatgtttccc cagtttccct gggtatcgat gaggagatct ttctgcccaa
     3181 gctgtacgag cgcatcgcct atggaagcat caccgtgggg gtgaaactgc atggtatgtc
     3241 ccacgatatt ggccaccttg ttgtaacgtc cgtcgtccag acgcttcatg gaatgcagaa
     3301 tacgtcgctg aaccggtttc agtccgtcgt gaatttcggg cacagcacgc tccaggatta
     3361 catacgaagc gtaatccaga aaccacgtct ggtacatccc cgaaaggtga tgtttcaccg
     3421 attcgtcgtc ttttattttc ggaatgatat agttggaatg agtttcaccc gcatcgtccg
     3481 tgtcagatgc ctgctccgca ttctccggac tctcatccgg gagatcctga tcctccatct
     3541 cgttcagttc atcctctttt gccataaatt tttcgcctgt catcccccac agaggaaaca
     3601 acatatttta attaacagtg aattattatc gtgaactatt acaaaataat ttacaatcga
     3661 caaagttaaa catttttttt aaaaataata agtaccttta cggctgtttg tgacgtaaaa
     3721 aagacaaaag ttatcaacaa tcaaatcatt ctccatgaag ccaaaattat tcattctgtt
     3781 tgctattgcg cttctttctg tttttacaaa ggctcaaacg gctgaaaaag gaaaaatcgg
     3841 cctgacttac tcattcaacg gcggcactac ggtcatgcag cctgccctcg aaggttctgc
     3901 aagtcacgat gtaacggatg ccactgcaat tggaatcata tacctgaagc cacttaacaa
     3961 ctggctcgaa ctggaaacgg gaatcaccta ttcgctctgc aaaattacca ctacatcagc
     4021 tcccactccc gaagtcaata cctacaaatc gaatttatcg ttgattgaca ttcctgtagg
     4081 tttgcgtgcc gtttttttga aatacttttt tgtgaacggg gctttgctgc tggatgccgg
     4141 attgatgaac aacagctccg taagcagcca gacaggtatt ggcatgatgg caggtgtcgg
     4201 cattaagtac gacttccggt tcgggggctc acttttcttc aatccgtacg ggaaaataca
     4261 ttcgcttgtg ccattcggcg ggtggaagca ccacgaccgc atactggaaa gcggctggaa
     4321 attagggatt acctgtgcac tttgatggaa tgagcaggaa ttgaatattt tttgtccggt
     4381 aaatccggtg aaaaaacctg aatgcatccg gcacggtgtt acaaatcagc gccggtggga
     4441 ggattaaagt ggtcgttatt ccatgcggtt acgttgttct gaataaattc ataaatacgt
     4501 tccggttcgt gctgactgca aataatatat tcgcggaaac gcggttgcca agcaaattcg
     4561 atgttgtttt cgcttgcaaa tccggtgact gcagatttga aactaccagt aacgtatgtc
     4621 aatcgaccac ttcaaacctg ctccggtgag aattaataac ctaatctgca taagacaatg
     4681 atatcaatgg gaaataaaaa ggagttatca ccggaacaac gggatgaaat aatcagcacg
     4741 ttgaaagacc gttttgagaa aaacatgaat cgccacaaag gtcttgaatg ggacggacta
     4801 ctggcaaggc tgaactccgg tacccgggat gaaaaactgt ggtcgcttcg tgaaatggaa
     4861 agaaccggag gtgaaccgga tgctgtagga tatgatgaaa aaacgggtga atacattttt
     4921 tatgactgtt cggcagaaag ccctaccggc cgcagaagtc tgtgttacga ccgcgaagcg
     4981 ctggaatcaa ggaaaacata taaaccggag aataacgcta ttgatatggc agccgatatg
     5041 ggtattgaac ttctgacgga agaacaatac agggaattgc aaaagcttgg aaatttcgaa
     5101 ctgaaaacat cgagctgggt gaaaacgccg gcagagatta gaaaacttgg cggtgcactc
     5161 ttttgtgatc gtcgctacga tcgtgtcttc gtgtatcaca acggtgcaga atcttattat
     5221 gccgtcaggg gttttcgcgg gtcactcagg gtttgatttt ttcccgagcc tctaccctgc
     5281 cggcagctcg attgtatcta aaatcgaacg gtttcgaagg aatttgaaga aatatggaat
     5341 cagtctgctg ttaatgtact gaatcaaaaa accgataaca ttaaagaaaa ctacggagta
     5401 agtgtaaaaa tgaattttcc acgtaataca atcaatcaac atcatataat ctgatatgaa
     5461 acagccatgc caatttattt ttgacacaca agtagatgat taatggcgtg ttaaatgtgt
     5521 ccttaataaa aataataaat aaacacatga aaaagttttg cctttcaatg ctgtttagca
     5581 tcgtaatttc catttctctc ttttctcaag gttatcaaaa tccggttttg cccggctttt
     5641 atcccgatcc
//
LOCUS       DAORKP010000135         5618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3022_length_5618_cov_9.288693, whole genome shotgun sequence.
ACCESSION   DAORKP010000135 DAORKP010000000
VERSION     DAORKP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_5618_cov_9.288693"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLN75_07650"
     CDS             complement(<1..1004)
                     /locus_tag="PLN75_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG04029.1"
                     /translation="MAVAAILTSCYDLKSRERLARAQQLLEQEYGDSAANALDSIYLP
                     RDMGREYYMQYLVTRVQAKYKAYRDVKNDTAVMEAVRYYEKKNKNPEQIALANYSAGC
                     VMKERGENKRAIPYFKKAVNAATLTKNFKLQGLIYGNLAYVYHEELFQEQAIDYFKKA
                     FSVYRQAKGTETNQMNTLSFLATNFLTNQQQDSALHYFKQALTIADAVHDRYYQAFIR
                     NNIGLVYQQKKDYNLSNFFLNESLHYSPDSSLRNKIQLNIAENYLKLTDFNALKEYLP
                     VIKKSMESTSDLYYKSATIDFLKEYEVRIGNYTQAFLYSEENYRIASTIFNQNQARAL
                     "
     gene            1405..5199
                     /gene="dnaE"
                     /locus_tag="PLN75_07655"
     CDS             1405..5199
                     /gene="dnaE"
                     /locus_tag="PLN75_07655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRG04030.1"
                     /translation="MGSFVHLHVHSSYSVLDGMSSISGLVDKAIGSGMNALALTDHGS
                     MFGIKEFYNYVKKKNDDKYAKIRELESKLKSDELPEPEKTALANELEATKLKIFKPIF
                     GSEVYVARRTRFDKELKEDRSGYHLVLLAKNEKGYRNLSKLVSYGWVQGHYYRPRIDH
                     DLLAQYSEGIIACSACLGGEIHKKVESGNMEEAEKAVLWYKKVFGDDFYLELQRHKTD
                     KPGADTETYVKQEIQNTELIRLARKTNTKLVATNDVHFVEEDHADAHEHLICLSTGKD
                     LDDPTRMRYTKQEWLKTPEQMKEIFADLPECIENTLEIADKVEYFKLESAPMMPVFPI
                     PKEFGTEEEYRQKFDEAALREEFGDAFERVGGYDKAIRIKLDSDFLTKITYEGAAKRY
                     GSNLNEETRERIDFELGVMKKMGFPGYFLIVQDFIQAARDMNISVGPGRGSAAGSVVA
                     YCLKITNIDPLKYDLLFERFLNPDRVSLPDIDIDFEDDKRGQVVRWVTEKYGKKRVAN
                     IVTYGTMGTKSAIKDVARVQKLPLSEADRLTKLVPGKFPKDENGKDIKVNLSNCFRYV
                     EELRESRDSSNPILSNTLKYAEMLEGTVRQTGVHACGLIIGADDLINLVPLSTAVDKE
                     TKEDKVITQYEGTVIEDVGLVKMDFLGLKTLSIIKDTLSNIRKSKGIDIDIDTIPIDD
                     KKTYKLFSRGDTVGVFQFESTGMQKYLKDLKPTQFEDLIAMNALYRPGPMEYIHQFVN
                     RKHGLEKIEYPFPVMEKRLKETYGITVYQEQVMLLSRDLAGFTRGQSDELRKAMGKKL
                     KDKMEALHAKFIEGAQKNGFGPTDKLEQIWADWAKFAEYAFNKSHATCYSLVAYQTAY
                     LKAHYPAEFMAANLTRNKDDITEVGKLMDECKAMKMSVLGPDVNESELTFTVNKEGNI
                     RFGLGGIKNVGDGAVEAIIHEREKNGKFKGIFDFVERVNMTACNKKTIESLAYAGAFD
                     AFTDIKREQFFTLNSREEQVMESLIKYGNLYQQDVLFNQNSLFGALDNAVEITKPEIP
                     FVTEWSSLVKLNREREMIGMYLSAHPMDEFEFEMNHICNATTVEITDLSKYHLGAGLK
                     LGGIVTNFRTGTTRTGKPFGMITIEDYQGSYEFPLFGKNYDDYGKYMIKDLFLYIHAV
                     VQEKGSDWSRKPASDLKPEIELKIITIMPFREVRKQIKRIRISLSVQKLNNDVVETLT
                     SLIKENKGDVSVYMDIKDFFTFEKVSLFSRPYRMKITSEVYKYLKYAEEEEILTYKIE
                     MN"
     gene            5320..>5618
                     /gene="trxA"
                     /locus_tag="PLN75_07660"
     CDS             5320..>5618
                     /gene="trxA"
                     /locus_tag="PLN75_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRG04031.1"
                     /translation="MALEFTDDNIKEIIESGKPVVIDFWAEWCGPCRMVSPIVEELAE
                     EFSGKVEIGKLNVDDNIQTPNEYGIRNIPTILFFKDGKMVDKQIGATPKASLKAKV"
BASE COUNT         1761 a         1233 c         1242 g         1382 t
ORIGIN      
        1 atcaatgccc gggcttgatt ttgattaaat atagtggatg caatacgata gttttcttct
       61 gaatacaaaa acgcttgcgt atagtttcct atcctgactt catattcctt caaaaagtca
      121 attgttgcac ttttataata gaggtcagaa gtagattcca tacttttttt gattacggga
      181 agatattctt tcagagcatt aaaatccgtt aacttcaggt agttttctgc aatatttaat
      241 tgtattttgt ttcttaaaga tgaatcaggg ctataatgta aagactcatt caggaaaaaa
      301 tttgagaggt tataatcttt tttttgttga tatactaatc ctatattatt tcgaataaat
      361 gcttgataat accgatcgtg aacagcatcg gcaatcgtaa gggcttgttt aaaataatgt
      421 aatgcactat cctgttgttg attggtcaaa aaattagtcg ccaaaaatga aagcgtattc
      481 atttgattag tctctgttcc ttttgcttga cgataaacgg agaaagcttt tttaaaataa
      541 tctatggctt gctcctgaaa taattcttca tgatacacat aagctaaatt cccataaatt
      601 aatccttgta atttgaaatt tttggttagt gtcgctgcat ttaccgcttt tttaaaataa
      661 gggatcgctc ttttgttttc ccctcgctcc ttcattacgc atcctgccga atagtttgcc
      721 agagctattt gttcgggatt tttgtttttc ttttcgtaat accgcactgc ttccattacg
      781 gccgtatcgt ttttcacatc acggtaagcc ttgtactttg cctgcacccg ggtaacaagg
      841 tactgcatat agtattcccg ccccatatcg cgcggcagat agatggaatc cagtgcattg
      901 gcagcgctgt cgccatactc ctgttccagt agttgctgtg cacgggcaag ccgctcgcgg
      961 cttttcaggt cgtagcagga agtgagaatg gcggcgaccg ccaccgtgaa aagaagcgta
     1021 agtaggatag aattttttcg tatcattgga cacaaaaata caaaaaaatc ttgtttggga
     1081 caaaagaata ttaatgaacg aaaagcaaca attcatctgt tttttctgct cacttctaaa
     1141 cgcatttagt ctcaactctc ctccaacaat atttaacaaa atcctatctc tttcattctc
     1201 tgaaagtacc ggggtatttc gtcggattgt caataacttt tctaaaaata attttccttc
     1261 cggtaaaaaa tccactatct ttatgtttcg attagttaaa gaagaatcca attctattat
     1321 actggttttt accgtgtcag gtagtgcatt gtggttcaaa atccaatcgt aaatcgtaaa
     1381 tttttaaatt gtaaattcta aaaaatgggt tcatttgtac atttacacgt tcactccagt
     1441 tactcggtac tcgacgggat gtcaagtatt tcgggattgg tagataaagc cattggcagc
     1501 gggatgaacg cgctggcact gaccgaccac ggaagcatgt ttggtatcaa ggaattttac
     1561 aattatgtga aaaagaaaaa tgatgacaaa tatgcaaaaa tcagggaact ggagtcgaag
     1621 ctgaagtcgg atgaactgcc agagcctgaa aaaacggctc tcgccaatga actggaggct
     1681 acgaagctga agattttcaa acctattttt ggctcggagg tgtacgtggc acgccgaacc
     1741 cgttttgata aagagctgaa agaagatcgc agcggttacc acctggtgct gctggccaaa
     1801 aatgaaaaag gataccgcaa cctgtcgaaa ctcgtttcgt acggctgggt gcagggtcat
     1861 tactaccgtc cgcgcattga ccatgattta ctggctcagt acagcgaggg aatcattgca
     1921 tgctctgcct gtcttggggg cgaaattcat aaaaaagttg aatcgggaaa catggaagaa
     1981 gccgaaaaag cggttctctg gtacaaaaaa gtgttcggcg atgattttta cctcgaactc
     2041 caacgccaca aaaccgataa accgggagcc gataccgaaa cctacgtgaa gcaggaaata
     2101 caaaacacgg aactcatcag gcttgcccgt aaaaccaata caaaactcgt ggcaacaaac
     2161 gatgttcact tcgtggaaga ggatcatgcc gacgcacacg agcacctgat ctgcctgagc
     2221 accggcaaag acctggacga ccccacccgc atgcgctaca ccaagcagga atggctcaag
     2281 actcccgaac agatgaagga aatctttgcc gatctgccag aatgcatcga aaacaccctc
     2341 gaaatagccg ataaggtgga atacttcaag ctggagtcgg cgcccatgat gcccgtattc
     2401 cccatcccca aagaattcgg caccgaagaa gaataccgtc agaaattcga tgaagcagcg
     2461 ctccgcgaag agttcggtga tgcttttgag cgcgtaggcg gatacgataa agctatacga
     2521 atcaaactgg actccgactt tctgacaaaa attacctacg aaggcgccgc gaaaagatac
     2581 ggctccaacc tgaacgaaga aacccgcgaa cggattgatt ttgaactcgg tgtaatgaaa
     2641 aaaatgggtt ttcccggata ttttctcatc gtgcaggact tcattcaggc tgcccgcgac
     2701 atgaatattt ccgtcggtcc cggacgcggt tcggctgccg gctccgtggt ggcttattgc
     2761 ctcaaaatca ccaacatcga tcccctgaaa tacgacctgc tgttcgaacg gtttctcaat
     2821 cccgatcgtg tttcactgcc cgatatcgac attgacttcg aggacgacaa gcgtgggcag
     2881 gtagtgcgct gggtgaccga aaaatacggc aaaaaacggg tggcaaacat cgtaacctat
     2941 ggcaccatgg gaacaaaatc tgccatcaaa gatgtggcac gtgtccagaa actgcctctt
     3001 tcggaagctg accggctcac caaactcgta cccggtaaat ttcccaagga tgaaaacgga
     3061 aaagacatca aggtaaattt gagcaattgt ttcaggtacg tggaagaact gcgggagtcg
     3121 cgcgattcgt cgaacccgat actctccaac accctgaaat atgccgagat gctcgaaggt
     3181 accgtacgcc aaaccggtgt gcatgcctgc ggactcatta tcggtgccga cgacctgatc
     3241 aatctggtgc cactgagcac agcagtggat aaagaaacca aagaagacaa ggtaatcacg
     3301 caatacgaag gaacggtgat tgaagatgtg ggattggtta aaatggactt cctgggactg
     3361 aaaactctct ccatcatcaa ggatacactc tcaaacatcc ggaaatccaa aggaatcgat
     3421 atcgacattg acaccattcc gatcgacgat aagaaaacat acaaactttt cagccgcggc
     3481 gatacggttg gcgtttttca gtttgaatct accggaatgc aaaaatacct gaaagacctg
     3541 aaaccaactc agtttgaaga ccttatagcc atgaatgcac tctaccgtcc gggtcccatg
     3601 gagtacattc accagtttgt gaaccgaaaa cacggcctgg aaaaaattga atatccgttc
     3661 ccggtcatgg aaaaacgcct caaggaaacg tatggaataa cggtatatca ggaacaggtc
     3721 atgcttctga gccgcgacct ggcaggattc acccgcggcc agtccgacga gctccgcaag
     3781 gccatgggga aaaagctgaa agacaagatg gaagccctgc atgcaaaatt cattgaaggc
     3841 gctcagaaaa acggattcgg accaactgac aagctcgaac aaatctgggc agactgggca
     3901 aaatttgccg aatatgcatt caacaaatcg cacgctacct gctactccct ggtggcctat
     3961 cagactgcat acctcaaagc acattacccg gcagagttca tggctgccaa cctcacccgc
     4021 aataaggacg acatcaccga agtaggcaaa ctgatggatg agtgtaaagc catgaaaatg
     4081 agcgtactcg gccccgacgt aaatgaaagt gagctcactt ttaccgtcaa caaggagggt
     4141 aatatccgtt tcggactcgg agggataaaa aatgtgggcg acggtgcagt ggaagccatc
     4201 atccacgaac gggagaaaaa cggaaaattc aaaggcatct ttgatttcgt ggaacgggta
     4261 aatatgactg cctgcaataa gaaaaccatc gaaagtctgg cttatgccgg tgcattcgat
     4321 gcttttacgg acataaaacg cgaacagttt ttcaccctca acagccggga agaacaggtg
     4381 atggaatcgc tcatcaaata cggaaacctc taccagcagg acgtactgtt taatcaaaat
     4441 tcgctgttcg gagcactgga caatgcggtg gaaatcacca aacccgaaat acccttcgtg
     4501 accgaatggt cgtcgctggt gaaacttaac cgcgaacgcg aaatgatagg tatgtacctc
     4561 tcggcacacc ccatggatga attcgagttt gaaatgaacc atatctgcaa cgctaccacc
     4621 gtagaaatta ccgacctgag caaatatcac ctcggcgccg gactgaagtt gggcggaata
     4681 gtgaccaatt ttcggacagg aactacccgg acaggaaaac cttttggaat gataaccatt
     4741 gaagattatc agggatccta cgaatttccg ctcttcggta agaattacga cgattacggc
     4801 aaatacatga tcaaggatct tttcctgtat attcatgcag tggtacagga gaaaggttcg
     4861 gactggagca ggaagccagc ttcggatctc aaacccgaaa ttgagttgaa aattatcacc
     4921 attatgcctt tcagggaagt gagaaagcag ataaaaagga taaggatttc cctatcggtt
     4981 caaaaactca acaacgacgt ggtagaaaca ctcacaagcc tgataaagga aaacaaggga
     5041 gacgtaagtg tttacatgga tataaaagat ttcttcacct tcgagaaagt gtcgcttttt
     5101 tcgcgcccat accgcatgaa aataacatca gaggtgtata aatacctcaa atatgctgaa
     5161 gaagaggaaa ttctgacata taaaatagaa atgaactgag ccaatagaaa gaattgataa
     5221 gattattgag aaaaaattca tcgtggcatt taaacatttt ctgaaaaaca acgttataga
     5281 aataattata aaaaaaatga attttaaaaa acgaagatta tggcattaga atttacagat
     5341 gataacatca aagagatcat agaatcggga aaacctgttg tgattgactt ttgggctgag
     5401 tggtgcggac cctgccgcat ggtaagcccc attgtagaag aactggcaga agaattctcc
     5461 ggaaaagtgg aaataggtaa actgaatgtg gatgataata tccaaactcc caacgaatac
     5521 ggcatccgca acatccccac catccttttc ttcaaagacg ggaaaatggt tgacaagcaa
     5581 ataggagcta cacccaaagc atccctgaaa gcaaaagt
//
LOCUS       DAORKP010000136         5542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3123_length_5542_cov_8.612174, whole genome shotgun sequence.
ACCESSION   DAORKP010000136 DAORKP010000000
VERSION     DAORKP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_5542_cov_8.612174"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            806..1672
                     /locus_tag="PLN75_07665"
     CDS             806..1672
                     /locus_tag="PLN75_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04032.1"
                     /translation="MARVKNLDQITGSLKNLSFYTRKGSDAIFVRTKGGPSKEKIKRN
                     PEFEGLRRINREFGGCSAMSKQIRQSFGELSHVADYNLSSALNSLMKSIQKTDEQHET
                     GSRSIRLSAFRYTLAGFDFGRKTRFSTLLRVPTGYNIDRENLTATVTIPAFACSFGLN
                     LKASPSQGAGNPGMFRISAALGIVTDMRLNNSGTAYEPVHDKLGHGIQLRSTPWFSTL
                     GSVQEQEIKLALNREYAVATDPLTTPPFVPEFTDADSLVLTIAVEFGTPDAFGNTVPI
                     RGTGGGMVLGVF"
     gene            1947..2609
                     /locus_tag="PLN75_07670"
     CDS             1947..2609
                     /locus_tag="PLN75_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04033.1"
                     /translation="MKKFAGKYRIQTARAPWWDYANDGAYFVTICTAHRECYFGDVVN
                     GMMKFNEMGEIANNIWDEIPRQFPYAELGVYQVMPNHVHGIIVINHTKCGDLGEKRDD
                     DDANDGGMRDDGTRGCRGAINRVSTGCNAETITSNADETPGGITGNHNPMLHDNLSRI
                     LRWYKGRVTFECHQTDVNFGWQARFHDNIIRDTASYNTISEYIINNPAKWDADKFHPS
                     NM"
     gene            2955..4697
                     /locus_tag="PLN75_07675"
     CDS             2955..4697
                     /locus_tag="PLN75_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04034.1"
                     /translation="MNIPLDEFEQHIDEDILKRGLQYFKKGYVTSVDELSGGEYEAIV
                     EGNDTYIVQLQVKNGSVIACNCTCPYDWGPVCKHEVAVMFYLQQDELGLKAKKKSTTK
                     TSPLSDVAGKEKKEVKKKKTIQEQLEEILEKLSPEEMRGYIGEYCDKDKTFRDLFLTQ
                     YLHLIQPVSKDIYTKQIRAVLKSASGRYGYIDYAGARQVGNSMHQLYQLAEKAVSAGN
                     WREAMYMGCAMLEEMTKAIEHGDDSNGDLGGSIDNAQNILFRIAQECTDSGIRSELFE
                     YSLDAYHRKLFEGWDWHYNMLDLAVELQQTEDEKTRILHYLDQIKPKNDGWDFRHEDA
                     QKQRLKLIRKTEGETCANDYLEANISNPDFRRELILKAIEEKNYAKAIRLAEKGIAAD
                     QKEKPGLADEWKWHLLNVYQLQNDPENVIEQARYLFLNSGRFSSKEMFHIMKKYVPAA
                     DWKLFFEQLVAEKIKSERWVSFHTIADMYIWEEQWENLFSLLIKNPSLDNIAYVEKYL
                     TKQYASQLVDLYCMAIQTFLEENVGRAHYKTACKYMRRMNKLGGREKTDILIAELRTK
                     YKPRRALMEELDKV"
     gene            5009..5446
                     /gene="tnpA"
                     /locus_tag="PLN75_07680"
     CDS             5009..5446
                     /gene="tnpA"
                     /locus_tag="PLN75_07680"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRG04035.1"
                     /translation="MPFIKVYIHFVWSTKNRVPYLNTPELRKGMWKHIRENAREKGIH
                     IDFINGFEEHCHCLVSLGDDQTMRKTMQLIKGESSYWINKEHLLNEKFEWQDEYFAVS
                     VSESLVDRVREYIKNQENHHVRKTWDEEYKDLIEKYGFERFKD"
BASE COUNT         1769 a         1083 c         1282 g         1408 t
ORIGIN      
        1 acctcaccat cgaaattacc gatccggccc gattctggag cgtctcgaaa tacctcgtgg
       61 ttcagaaata gttatagatt tcgaagcgga aacaggggga atgatttgcc gatacaacta
      121 tctcaggtga gattcagttt ccgatattat cagtaacagt aacctcaaag agataaaata
      181 acttacacaa aaattcagga aaacagcgtg gagcaacgaa atccacgctg ttttttgttt
      241 atttccctcc tttcggtacg catgttcttt aattcatgcg ctgttcttgg tgaatggctg
      301 tttatcgggc attgcattca gatttactgt atatcaccaa gagtgaaaaa tggatgagat
      361 gagacccgta aagcgatttg catcggtacg cagaaacgcc tgcaacatga agagttcagg
      421 caggaatcac tcccgaagta atacttcaat cagaattaaa caaatgttat cctgcaattc
      481 tccggggttt ttgtccacat gttttaactg tttcaggaat ttcatttgcc cgtatttgta
      541 tgtctgatga gtatgtgctg atatcaatat attacagata tattacacat atatcacaca
      601 tataaaacac atttgtttca cattttaatc tgaaatatat ctgaatctat tctttgttta
      661 atgtttattt tatatatttg taattacatt aagaacacac tcttctatct gtttgctgcg
      721 acactgagag gctttccaga tacagacagg cagagatgtt tttgatagta cgattaaaca
      781 ggaatttttt ttaaaaaata cgattatggc acgggtaaaa aatctggatc agataacggg
      841 cagtctgaaa aacctgtcgt tttacacacg taaaggcagc gatgcaatat ttgttcgcac
      901 caaaggcgga ccttccaaag aaaaaatcaa acgcaatccc gaatttgaag gattgagacg
      961 aatcaaccgc gagttcgggg gatgttctgc catgagcaaa caaatacggc aatcgttcgg
     1021 tgaactgagc catgtggcgg attacaacct gtcatccgca ctgaattcgc tgatgaaaag
     1081 catccagaaa accgacgagc agcacgaaac cggcagtcgc tctatccgct tgtcggcttt
     1141 caggtacacg ctcgcgggct ttgatttcgg gcggaaaacg cggttcagca cgctgctgcg
     1201 ggtgccgacc gggtacaaca tcgacaggga gaacctgaca gcgacggtga cgataccggc
     1261 tttcgcgtgc agtttcgggt tgaacctgaa agcaagtccc tcccagggtg caggcaaccc
     1321 gggtatgttc cggattagcg cggcgctggg aattgtaacc gatatgcggc tcaacaactc
     1381 cggcactgcc tacgaaccgg ttcacgataa gctggggcat ggcattcagc tccggagtac
     1441 accgtggttt tccacactgg gcagtgtaca ggaacaggaa ataaagctcg cactgaacag
     1501 ggaatatgcc gttgcaaccg acccgctcac taccccaccc ttcgtgcccg aattcaccga
     1561 tgccgacagc ctggtgctga caatagccgt tgaattcgga acacccgacg cattcggcaa
     1621 cacggtgccg atacggggca cgggaggagg aatggtgttg ggagtttttt agtttgatgt
     1681 ttgtagtttc aggtcatacc caattatgtt catgggataa ttgtaattaa ttatacgaaa
     1741 tatggggatt tgggagaaaa acacgggacg atgatgaaat aaatgatgac gggatgcgcg
     1801 attgtagaga cgcgattaat cgcgtctcta cggtgtgcaa cgcgggaatt atcgcgtcca
     1861 acgcggaaac catcgcgcac atcacggatc atcccgacaa tcattatccg aaaaaaaaat
     1921 aaaacaataa taataacaaa ccaaccatga aaaaatttgc cgggaaatac cggatacaga
     1981 ccgcacgtgc accgtggtgg gattatgcca acgacggagc gtattttgtt accatttgta
     2041 cggcacaccg cgaatgttat tttggcgatg tggtaaacgg catgatgaaa ttcaatgaaa
     2101 tgggtgaaat tgccaataat atttgggatg aaataccccg gcaatttcca tacgccgaat
     2161 tgggggtata tcaggtgatg cccaatcacg ttcatggaat tattgtaatt aatcatacga
     2221 aatgcgggga tttgggagaa aaacgtgacg atgatgatgc caatgatggt gggatgcggg
     2281 acgatgggac gcgcggttgt agaggcgcga ttaatcgcgt ctctaccgga tgcaacgcgg
     2341 aaaccatcac gtccaacgcg gacgaaacgc ccggcggtat caccggcaat cacaatccga
     2401 tgttgcatga taatttatca cgcattttgc gttggtacaa aggtcgtgtt acatttgaat
     2461 gccaccaaac agatgtaaat tttggatggc aagcacggtt tcatgataac attatccggg
     2521 atacggcatc atacaacacc atttcggaat acattataaa caacccggca aaatgggatg
     2581 cggacaaatt tcatccgtca aatatgtaga ggtgcgatta atcacgtatc tacataattg
     2641 attaatcgcg tcaatttttc gaaatatggt tgtgattcac ggtcgtagag gcgcgattaa
     2701 tcgcgtatct actaaccggt ggatgaatat agaaatatcg taatagaaat aataaaatca
     2761 atttatattt cggtgggttg cacctgtgaa attgttgttc tatatttgtt ttcaacataa
     2821 cgcccaactg tcaccggatg cactgccgtt tcttcaggat taccgatgaa acgacgttta
     2881 caggaaacct gacatataaa accaaatttc gaaaaaaaac cagcaatata accaaattat
     2941 aattccaacc aactatgaat attccattag acgaatttga acagcacatt gatgaagata
     3001 ttctgaaacg cggtctccag tacttcaaaa aaggctatgt tacgagcgtg gatgaactaa
     3061 gcggaggtga atacgaggca atcgttgagg gcaatgatac ttacattgtt cagcttcaag
     3121 tgaaaaacgg cagtgtaatc gcctgcaact gtacctgccc ctacgattgg ggaccggttt
     3181 gcaagcacga agtagctgtc atgttctatc tgcagcagga tgaacttggg ctgaaagcga
     3241 aaaagaaatc caccaccaaa acatctcccc tttcggatgt ggcaggtaag gagaaaaaag
     3301 aggtcaaaaa gaaaaagacc attcaggagc agctggaaga aatacttgaa aaactctccc
     3361 cggaagaaat gagaggatac attggggaat attgcgacaa ggacaaaacg tttcgcgatc
     3421 tttttctgac acaatattta cacctgatac aaccggtatc gaaggatata tataccaaac
     3481 aaatacgggc tgttttgaaa tcggcatctg gaaggtatgg atacatcgat tacgcgggag
     3541 cccgacaggt gggcaactcg atgcatcagt tgtatcaact ggccgaaaaa gccgtgagtg
     3601 ccggcaactg gcgtgaagcc atgtacatgg gttgcgctat gctggaggaa atgaccaaag
     3661 cgatagaaca cggcgacgac agcaacggcg atctgggtgg cagcatcgat aatgcacaga
     3721 atatcttatt ccgaatagct caagaatgta ccgacagcgg tattcggagt gaactttttg
     3781 agtactcgct cgacgcttat caccgcaaac tgttcgaagg atgggactgg cactataata
     3841 tgctcgatct ggctgttgaa ttgcagcaaa cagaagatga aaagacgcgg attttgcatt
     3901 atcttgacca gattaaaccc aaaaatgacg gttgggattt tcgccatgaa gacgcacaga
     3961 aacaaagact gaaacttatc cggaaaacgg agggagaaac ctgcgctaac gactatctgg
     4021 aggccaatat atccaaccct gatttcagac gtgaactgat actgaaagcc attgaagaaa
     4081 aaaactatgc caaagctatc aggctggctg aaaaaggaat tgcagccgac caaaaggaaa
     4141 aacccggact ggcagacgaa tggaaatggc acctgctgaa cgtttatcaa ttacagaatg
     4201 acccggaaaa cgtaattgaa caggcccgtt atttattttt gaattcaggc aggttcagtt
     4261 cgaaggaaat gtttcacatc atgaagaaat atgtacctgc tgccgattgg aaacttttct
     4321 tcgaacagct ggttgcggaa aagatcaaat ccgaacgatg ggtttctttt catacgattg
     4381 ccgatatgta tatttgggaa gaacaatggg aaaacctgtt cagcctgctg ataaaaaacc
     4441 cttcgcttga taatattgcc tacgtggaaa aatacctgac aaaacagtat gcttcgcaat
     4501 tggttgatct gtattgcatg gcaattcaga cattcctgga agaaaacgta ggcagagcac
     4561 attacaagac agcctgtaaa tatatgcgcc ggatgaataa actgggcgga cgtgaaaaaa
     4621 cggatatcct gatagctgaa ttgcgcacaa aatataaacc gcgacgggca ttgatggagg
     4681 agttggataa agtatagctt cgtttcacgt ttaacgtttg aggtttaatg tttcgcgttt
     4741 catgtttcat gtttcatgtt tcacgtttga ggttattgag ttattgagtt gagtatttat
     4801 gcaatacaat atgaattgga ttgggcttta gcccaatcat ataaattata atatcaaata
     4861 tgggctttag ccaaaggatt tataatgaat atattttgca atagaattgg gataataccc
     4921 attcaaaaca atattaaaat caaataggaa ctttagccaa atctctcaaa aaataagcat
     4981 gaaaatcatt attaaataaa tcaaaattat gccatttata aaggtttaca ttcattttgt
     5041 ctggtcaaca aaaaacaggg tgccttatct gaacacccct gaattgagaa aaggtatgtg
     5101 gaaacatatc cgggaaaatg cccgggagaa aggaattcat attgatttca tcaatggttt
     5161 tgaggaacat tgtcattgcc ttgtttcttt gggagatgat caaacaatga ggaaaacaat
     5221 gcaattgatt aaaggagaat catcatactg gattaacaaa gagcatttgc tgaatgagaa
     5281 gttcgaatgg caggatgagt attttgctgt atcggtatct gagtcgctgg tggatagagt
     5341 aagggagtac attaaaaacc aggaaaacca tcatgtccga aaaacatggg atgaggaata
     5401 caaagattta atagagaaat atggatttga aagatttaag gattaaataa tttggctaaa
     5461 gccaatacaa ggaaaatgaa tttgttgtcc tccggctaaa gccggaggca attcaggagg
     5521 caattcagga ggcaattcag ga
//
LOCUS       DAORKP010000137         5481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3202_length_5481_cov_7.316255, whole genome shotgun sequence.
ACCESSION   DAORKP010000137 DAORKP010000000
VERSION     DAORKP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_5481_cov_7.316255"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLN75_07685"
     CDS             <1..681
                     /locus_tag="PLN75_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRG04036.1"
                     /translation="YPYLHGESIRKAGFSYVSCSEKSVTDGDVKLHCYPFVDLILGKE
                     REWTSLNKEATQPVFRTFTPEMQQAIMQYTDSGGNLLVSGSNIISDLVLSKNSTDRDR
                     SFVENTLKIKWMSDKINDLHKVNFNNNEINHFKDLNVSFHASPNEESYYVEAPDIITP
                     NGKFAYQICKFEENNQGAGIVYDGNYRICALGFPFETIRDDHDRNKLMKYVLNFFSDT
                     NRNIISYK"
     gene            692..1765
                     /gene="pdxA"
                     /locus_tag="PLN75_07690"
     CDS             692..1765
                     /gene="pdxA"
                     /locus_tag="PLN75_07690"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRG04037.1"
                     /translation="MEPEKIIIGITHGDINGIGYEVILKALSDNRVLDMFVPVIYGSS
                     KLAGFYKKNLDLQGLNLNVINSLEEANSKRINIINCVDEELKVELGQSTAEAGKAAYE
                     ALEKATEDLKKGRLHAIVTAPINKKNIQSADFHFPGHTEYLETKFGEQSSALMMLVND
                     FMRVAVVTGHIPVSNVASVITEDLIVEKLKILNSSLKKDFSIVKPRIAVLGLNPHAGD
                     DGVIGNEEQNVIIPALKKADSQGILCFGPYPADGFFGSGNFRNFDAILAMYHDQGLIA
                     FKTLSMESGVNYTAGLSVIRTSPAHGTAYTLAGQNKASEQSFLEALYLACDIYKNRLE
                     YIAMNANPLVIEPKRENHKRNIE"
     gene            complement(1857..3680)
                     /locus_tag="PLN75_07695"
     CDS             complement(1857..3680)
                     /locus_tag="PLN75_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRG04038.1"
                     /translation="MDAPDTMNEMDNLNESLITPEEIQEVTTEPILEVKPEAPVAHDL
                     TKQEICDRMKELIEQNVEQVKEEVESMKQLFYKKSKSEVGEQRAAFIEKGGVETEFIP
                     EPTELEAEFKELLNEFRNNKAKLNAQQEQEKGNNLLKKQHIIEQMKQLVESNDDVSAN
                     VAAFRQLQQDWKNTGPVPPTATNELWKQYNLYQESFWDLIKINNELREYDFKKNLEAK
                     TKLCEAAEMLANEEDVVSAFRQLQKLHENWHELGPVARELREEIWNRFKTASTTINKK
                     HQSHFDDIRKVEDENMALKASLCERIEAIDTTKFSSYKDWDEATKIVLEAQEEWRSVG
                     FAPRKANQKIFDRYRKACDKFFSTKAEFYKSIKSNLTQNLEQKKLLCEKAEAIKDSTD
                     WKEAGDQLVQLQKEWKTIGPVAKKYSDDIWKRFITACDYFFEQKNKNASGQRSVEMQN
                     LDKKKELIAKITGFEKTGNAAESLAALRALAAEWNALGHVPFKEKDKIYKEYRAALDA
                     QFEKLNIDVSQRRLETFRNNLKDMTSKGDNKLYREREKLVRAYEHLKSEIATYENNIG
                     FLSIASKKGDGMLKEMERKIETLKEECKLLEQKISLIDDQM"
     gene            complement(3848..4606)
                     /locus_tag="PLN75_07700"
     CDS             complement(3848..4606)
                     /locus_tag="PLN75_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRG04039.1"
                     /translation="MNRTAIVTGATSGFGRAIALRLAGLGYRLIVTGRRKELLDTLTA
                     EISEKYSVSVLALNFDVRDFKACEEAVNSLPDEFKSIDLLVNNAGLAAGAAPFDQSLL
                     DDFDRMIDTNVKGLLYMTKLIVPGMIERQAGHIVNISSIAGIEVYPSGSVYCASKHAV
                     NAITKGLRIDLVKHGIRVSSISPGMAETEFSIVRYHGDTEKAKAVYNGLTPLNAEDIA
                     DALEFIVTRPAHVSINDIQINPSQQANTYISHRK"
     gene            complement(4603..>5481)
                     /gene="ribA"
                     /locus_tag="PLN75_07705"
     CDS             complement(4603..>5481)
                     /gene="ribA"
                     /locus_tag="PLN75_07705"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRG04040.1"
                     /translation="ASTPETFGRPGHIFPLRARSKGVLRRAGHTEAAVDLARLAGLYP
                     AGALIEIMNEDGSMARLPQLFELADQFKMKIITIADLIAYRIRTESLIEKGDEVDMPT
                     AYGHFKLIPFRQVSNGLEHVALIKGDWEKDEPVLVRVHSSCMTGDIFGSMRCECGEQL
                     HRAMQLIEKEGKGAIVYMNQEGRGIGLMNKIKAYKLQEEGMDTVDANLHLGFRADERD
                     YGVGAQILREIGVSKIRLMTNNPKKRIGLEAFGLSIVENVPIEVKPNEYNEFYMLTKK
                     DRMGHKLKYVGKDNVK"
BASE COUNT         1446 a         1199 c         1136 g         1700 t
ORIGIN      
        1 tatccatacc tacatggaga aagtatccga aaagccggat tttcatatgt ttcatgcagc
       61 gaaaaatcgg taaccgacgg agacgtgaag ctgcactgct accctttcgt agatttgata
      121 ctgggcaaag agcgcgagtg gacaagtttg aacaaagagg ccacacaacc cgttttcaga
      181 acttttaccc ctgaaatgca acaagcgatc atgcaataca ccgattcggg tggtaatctg
      241 ctggtaagcg gttcaaatat tatttcagat ttggttttat caaaaaacag caccgatcgg
      301 gatcgctcct ttgtggaaaa cacgctgaaa atcaaatgga tgtcggataa aataaacgac
      361 cttcataagg tcaattttaa taacaacgag attaatcatt ttaaggattt aaatgtctca
      421 tttcatgctt ccccgaatga agaatcgtac tatgtggaag cgccggatat cattaccccg
      481 aacggtaaat tcgcttatca gatttgcaaa tttgaagaga acaatcaggg agcaggcatc
      541 gtatacgatg gaaactaccg gatttgtgcg cttggatttc ccttcgaaac catcagagac
      601 gatcatgacc gcaataaact gatgaaatat gtgctgaatt tcttttcgga tacaaacaga
      661 aacatcattt catacaaata aaataaataa tatggaacca gaaaaaataa taatcggaat
      721 aacacacggt gatatcaatg gcataggcta cgaagttatt ctaaaagcct tgtcggacaa
      781 tcgggtactg gatatgtttg taccggtgat atacggctcg tctaaactgg caggttttta
      841 caaaaaaaat cttgatttgc agggactaaa tctgaacgtg atcaattcac tcgaagaagc
      901 aaattcgaaa cggatcaata tcatcaattg cgtggacgaa gaattgaaag tcgagttggg
      961 acagtctact gctgaagccg gtaaagcggc ctacgaagca ttggaaaaag cgactgaaga
     1021 tttaaaaaaa ggaagactac atgcaatcgt tacggctccc ataaataaaa aaaatattca
     1081 atccgctgat tttcattttc ccggtcacac cgaatatctg gaaacaaaat ttggtgaaca
     1141 atcatcggct ctgatgatgc ttgtaaatga tttcatgcgg gtggctgttg tgaccggcca
     1201 tatacccgtc agcaatgttg catcggttat tacggaagac ctgatagtgg agaaactgaa
     1261 gatactgaat agttcgctga aaaaagattt ttcaattgtc aaaccccgga ttgctgtttt
     1321 gggtttgaat ccccatgcag gtgatgatgg tgtaatcggt aatgaagagc aaaatgtaat
     1381 aattccagcc cttaaaaagg ctgacagtca gggtatactt tgttttgggc cttaccctgc
     1441 cgatggtttt tttggatcgg gtaatttccg taatttcgat gccattctag ccatgtatca
     1501 cgaccagggt ctgatagctt ttaagaccct gtccatggaa agcggtgtaa actatacggc
     1561 cggtttatcg gttatacgca catcgcccgc tcacggtact gcctacacgc tggccggcca
     1621 aaacaaagca tcggagcaat cttttctgga agcactgtat ctggcttgcg atatttacaa
     1681 aaacagactg gaatacattg ctatgaatgc aaatccactg gtaattgaac caaaaaggga
     1741 aaaccacaaa agaaatattg aataaaactc tccaccaatg atggagttca tcctattaca
     1801 acagaaaagc accggaattt cccggtgctt ttctgttgga taataatata agatatttac
     1861 atttgatcgt caatgagtga tattttctgc tccagcagtt tgcattcctc tttcaatgtt
     1921 tcgatttttc gctccatctc tttgagcatt ccgtcgcctt ttttggaggc aatgctgagg
     1981 aaaccgatat tattttcgta ggtggcaatt tcagatttga gatgttcgta agctctgaca
     2041 agtttttcac gctcccgata cagtttgttg tctcctttgg aggtcatatc cttcaggtta
     2101 ttgcggaatg tctccaacct acgctgcgaa acatcaatat tcagcttttc aaactgagca
     2161 tcgagtgcgg cacgatattc tttgtatatt ttatcttttt ccttgaaagg aacatgacct
     2221 aaagcattcc actcggcagc cagtgcacgc agggcagcca gcgattcagc ggcgttacct
     2281 gttttttcaa aaccggtaat ttttgctatc agctcttttt tcttgtcaag attttgcatt
     2341 tctacactgc gctgaccgga tgcatttttg tttttctgtt cgaagaaata atcacaggca
     2401 gtaataaatc gtttccagat atcatcggaa tatttcttgg ctaccggtcc tatggttttc
     2461 cattctttct gtaactgtac cagttgatca cccgcttctt tccagtcagt actatcctta
     2521 atggcttctg ccttttcaca gagtagtttt ttctgttcaa gattctgtgt cagatttgac
     2581 tttatggatt tataaaattc agctttggtg gagaaaaact tatcgcaggc tttgcggtaa
     2641 cgatcgaaaa ttttctgatt agccttgcgt ggtgcaaaac ccacagagcg ccattcttcc
     2701 tgtgcttcaa gtacaatttt tgtggcttcg tcccaatcct tgtacgaact gaatttggtg
     2761 gtatcgatgg cttcaattct ttcgcacagc gatgccttca acgccatatt ctcgtcttcc
     2821 accttgcgga tatcatcaaa gtgcgactga tgttttttat ttattgtagt ggatgctgtt
     2881 ttgaatcggt tccagatttc ttcgcgaagc tcgcgtgcta ccggaccaag ttcgtgccag
     2941 ttttcatgca gtttttgcaa ctgacggaaa gccgaaacca catcttcttc gttagccaac
     3001 atttcggcag cttcgcacag tttagttttt gcttccagat ttttcttgaa atcgtattcc
     3061 ctgagttcgt tgtttatctt gatcagatcc cagaaagact cctgatacag gttgtattgt
     3121 ttccaaagtt cgttggttgc tgtaggaggt acaggaccgg tgtttttcca gtcttgctgc
     3181 aattgacgaa atgcagctac attggccgac acatcatcgt tactctcgac cagctgtttc
     3241 atttgctcaa taatgtgctg ttttttcagc agattattgc ctttctcctg ttcctgttga
     3301 gcattcagtt ttgctttatt gtttctgaat tcatttaaga gttctttgaa ttcagcttca
     3361 agttcggtcg gttcgggaat aaattcagtt tcaactccac ctttttcgat gaaagcagcc
     3421 cgttgttcac caacttctga tttactcttt ttatagaata attgtttcat ggactcaact
     3481 tcctctttga cctgctccac attctgttcg ataagttctt tcatccggtc acagatttcc
     3541 tgtttagtga ggtcatgtgc gactggagct tcgggcttta cttccagtat tggttcggtt
     3601 gttacttcct ggatttcctc gggcgtaatg aggctttcat tgaggttatc catttcattc
     3661 atagtgtcag gtgcgtccat aatctgttgt tttttagtat gtcaaagtta ttttaccttc
     3721 aacatgggtg tccaattcat ttcaaaataa aaattttgca accacaaaaa taagataaat
     3781 tttcaaataa tttaatcctt ttgttataat ttctttagat gtttaatttt ccgacagttt
     3841 tatattttca ttttcggtgc gaaatatagg tatttgcctg ttgtgaggga ttaatctgaa
     3901 tgtcattaat gctgacgtga gcaggccttg taactataaa ttccagtgca tcggcaatgt
     3961 cttcggcatt cagcggggtc aatccgttgt acactgcttt ggctttttcg gtatcgccat
     4021 gatatctcac tatagagaat tcggtttcgg ccatgccggg agagatagaa ctgaccctaa
     4081 tcccatgctt caccagatca atccgaagcc ctttggtaat ggcattgacc gcatgtttcg
     4141 aagcacagta gacacttccg gacggataaa cttcaatgcc ggcaatggac gagatattga
     4201 ctatatgacc agcctgacgt tcaatcattc cgggtacgat cagcttagtc atatacagaa
     4261 gtcctttgac attggtatcg atcatccggt caaaatcatc gagcagactt tggtcaaaag
     4321 gtgctgcacc agcagcgagc ccggcattat tgacgagcag atcaatactt ttgaattcgt
     4381 cgggcagcga attgacggct tcttcgcatg ccttaaaatc acgcacatca aaattcaaag
     4441 ccaatacgct gacagaatat ttttcactga tttcggcggt gagtgtatcc agtaattcct
     4501 ttcgccggcc ggttacaatc aaccgataac ccagcccggc aaggcgaagt gcaattgcac
     4561 gtccaaatcc ggaagttgct ccggttacaa tagctgtcct gttcatttca cgttatcttt
     4621 tcctacgtat ttcagtttat gccccatccg gtcttttttt gtcaacatat aaaactcgtt
     4681 atactcattg ggcttcactt caatgggcac attttcaaca atggagagtc cgaatgcttc
     4741 gagcccaatc ctttttttgg gattatttgt catcaaccgg atttttgaca caccaatttc
     4801 gcgaagtatc tgtgctccca cgccgtagtc gcgttcgtcg gcccgaaacc cgagatgtaa
     4861 attggcatct accgtatcca ttccttcttc ctgcagttta taggccttta tcttattcat
     4921 cagtccaatg ccacgacctt cctggttcat atacacgata gctccctttc cctccttttc
     4981 gatcaattgc atagctctat gcaactgttc gccgcattcg catcgcatgg atccaaatat
     5041 atcgcccgtc atacaggatg aatgtactct aaccagcacg ggttcatcct tttcccagtc
     5101 acccttgatg agggcaacgt gttccagccc gttggatacc tgtcggaacg gtattaattt
     5161 gaagtgtccg taagctgttg gcatatccac ttcgtcgcct ttttcgatga gtgattcggt
     5221 tcggatacga taggctatca ggtcggctat agtaataatc ttcattttga actggtcagc
     5281 aagttcaaaa agctgaggta aacgtgccat ggagccatct tcattcatga tttcaatgag
     5341 ggcacctgcc ggatagagac ctgccagacg ggcaagatca acagccgctt cggtatgacc
     5401 ggcgcggcgc agaacgcctt ttgagcgggc tctcagaggg aatatatgtc cgggtcgtcc
     5461 gaacgtttcg ggcgttgatg c
//
LOCUS       DAORKP010000138         5419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3277_length_5419_cov_9.555183, whole genome shotgun sequence.
ACCESSION   DAORKP010000138 DAORKP010000000
VERSION     DAORKP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_5419_cov_9.555183"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..809
                     /gene="rnc"
                     /locus_tag="PLN75_07710"
     CDS             42..809
                     /gene="rnc"
                     /locus_tag="PLN75_07710"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRG04041.1"
                     /translation="MKFNPLHIFRLLSKNRQEPFLSFSHILGFNPGNIELYEIAVRHR
                     SVPFKNKNGEAVNNERLEFLGDAVLNSIISDILYHRYGNKQEGFLTNARSNIVKRDSL
                     NKMCHQTGLDKLVVADKQLNLKENANIYGNALEAFIGAIYLDAGYDKCARFVKQRLLI
                     SPETMQILVEDNENFKSALLEWCQQRYLTLDFDLVEETVDSHNRHTFTSQVLINNMPI
                     CTGSGANKKESHQRASAQALRMIRNDENFTNTFGLTE"
     gene            943..2745
                     /locus_tag="PLN75_07715"
     CDS             943..2745
                     /locus_tag="PLN75_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRG04042.1"
                     /translation="METTKPFQRLIQIQAEKYGDKPALYDRNKLTDPWNSISWNRMND
                     MVNHLAKALLELGVKPQDRIGQYSQNKAENLLVDYATFSIRGVVVPIYPTSTQQQVDF
                     ITKDAAVEILFVDGQKQYEAALEVMKTSTHLKKIIVLEQQVKLADSTHSMYFEDLIEI
                     GMKSKKDDEVEKRRAESVEEDMGCLLYTSGTTGNPKGVVLNFVMFNEAVRIHLIRLSQ
                     VNDSDTSLAFLPLTHVFERMWCYFLLQVGTTVYINHNPTEIQTTLKEVRPSLMCAVPR
                     FWEKVYIGVKEVLSGYSPLKLAIVTWATAVGKKHNIDYLRKGKKPDFLLKFYYRLAEK
                     MVFSKLKTTLGLENAKILPVAGAKLSDEITLFFRSIGVPVVYGYGLTESTATVTCFED
                     EYEIGTVGSIMPDVEVKIGKDNEILLRGKTITPGYYNNPEANKKAFTDDGWFCTGDAG
                     TIRDNKIVLTERLKDLFKTSNGKYIAPQEIETRVSTDKFIEQVAVIGDERNYVTAIIA
                     PSIPALEKYAKEHKIVYQDVDELLRLPEIKKLIEDRIAEHQQGMASYEMIKKITLIKI
                     PFTIEAGELTNTLKIRRSVITQKYKNEIDAMYEA"
     gene            3041..4033
                     /locus_tag="PLN75_07720"
     CDS             3041..4033
                     /locus_tag="PLN75_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRG04043.1"
                     /translation="MVSYKELGLVNSKELFRKAIEGKYAIPAFNFNNLEQLQAIVSAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVQYAKELGYEIPIVLHLDHGDSFELCK
                     DCIESGFSSVMIDGSHLPYEENIALTKKVVEFAHAHDVTVEGELGVLAGIEDDVVAEH
                     HTYTEPEQVVDFVSRTGVDSLAISIGTSHGANKFTPAQCTRDENGVLIPPPLRFDILE
                     EIEKQIPGFPIVLHGSSSVPQEYVATINKFGGALKDSIGIPEEQLRHAAESAVCKINI
                     DSDGRLAMTAAVREVFATKPGEFDPRKYLGPARDELKKLYMHKTQSVLGSAGKA"
BASE COUNT         1659 a         1260 c         1196 g         1304 t
ORIGIN      
        1 ggcgttaatt tcaaaacgat tgaaagcctt tttgtatata aatgaaattc aatccgttac
       61 atatattcag gctcttgtcg aaaaaccggc aggagccttt tttgtcgttc agccatatac
      121 tgggattcaa tccgggcaac atcgaactgt acgaaatagc ggtgagacac cgttcggttc
      181 cgttcaaaaa caagaacggc gaagcagtta acaacgaacg gctcgaattt ctgggcgatg
      241 cggtactcaa ttcaatcatt tcggatatcc tgtatcaccg ctacgggaat aaacaggagg
      301 gatttctgac caatgcccgt tcgaacatcg ttaaacgaga ttcgctcaat aaaatgtgcc
      361 accaaaccgg actcgataaa ctggtagtgg ccgataaaca actgaatctg aaagagaacg
      421 ccaacattta cggtaacgca ctggaagcgt tcattggagc catatacctc gatgcgggtt
      481 acgataaatg cgcccgtttt gtaaagcaac ggttgcttat ttctcccgaa acgatgcaaa
      541 ttctcgttga agataacgag aacttcaaat cggcattgct cgaatggtgc cagcagcgct
      601 atctgacgct tgatttcgat ctggtggaag aaacggtgga cagtcacaac cggcatacat
      661 tcacttcgca ggtactcatt aataatatgc ccatctgcac aggcagcgga gccaataaga
      721 aagaatcgca tcagcgggca tcagcgcagg cgctgcgtat gatccgaaac gacgaaaact
      781 ttacaaacac cttcggcctg accgaataag atgtacctct tccggttcaa acctgtaaca
      841 ggaggttttt agctgttacg tacaattcaa tccgaatcag tttaaacttt atgaaacttt
      901 catctgtttc attcacagac aatacaatca aaaaaagaaa ttatggaaac gacaaaaccc
      961 tttcagagac ttatacaaat tcaggcagaa aaatacggcg acaaacccgc actgtacgac
     1021 cggaacaaac tgaccgaccc ctggaattcc atcagttgga accggatgaa cgacatggta
     1081 aatcacctgg cgaaagccct gctggaactg ggtgtaaaac cgcaggaccg catcggacag
     1141 tattcgcaaa acaaagccga gaacctcctg gtggattacg ccactttcag catccggggt
     1201 gtggtggtgc ccatctaccc tacttccaca cagcagcagg tggactttat caccaaagat
     1261 gccgcagttg aaattctgtt cgtggatgga caaaaacaat acgaagcggc gctggaagtg
     1321 atgaaaactt ccacgcatct gaaaaaaatc attgtgcttg agcagcaggt gaaactggcc
     1381 gattccactc attccatgta tttcgaggat ctgatagaga taggaatgaa atcaaaaaaa
     1441 gacgacgagg tagaaaaaag aagagccgaa agtgtggagg aagatatggg ctgcctgctc
     1501 tacacatcgg gcactacggg taatccaaaa ggggttgttc tgaatttcgt tatgttcaac
     1561 gaagcggtac gcattcacct catcagactg tcgcaggtca acgacagcga cacttcgctg
     1621 gcatttctgc cgctcaccca cgttttcgaa cgaatgtggt gttatttcct gcttcaggta
     1681 ggcactaccg tgtacatcaa tcacaacccg accgaaatcc agaccacgct caaagaggtg
     1741 cgcccctcgc tcatgtgcgc cgttccgcgg ttctgggaga aagtatacat aggcgtcaaa
     1801 gaggtgctga gcggttactc gcctctcaaa ctggccatcg taacctgggc tacagccgta
     1861 ggcaagaaac acaacatcga ctacctgcgt aaaggcaaaa aaccggattt cttactcaaa
     1921 ttctactacc ggctggccga aaaaatggtt ttctcgaaac taaaaaccac actgggactc
     1981 gagaatgcaa agattctgcc cgtagccggt gccaaactct cagatgaaat caccctgttt
     2041 ttccgtagca tcggtgtacc cgtagtttac ggatacggat tgaccgaatc gaccgctacg
     2101 gtcacctgct ttgaggacga gtatgaaata ggaaccgtag gctccattat gccggatgtt
     2161 gaggtaaaaa tcggtaagga taacgaaatc ctgctgagag gaaaaaccat taccccgggt
     2221 tattacaaca accccgaagc caataaaaaa gcgtttaccg atgatggctg gttctgtacg
     2281 ggcgatgccg gcacaatcag agacaataaa attgtgctca ccgagcggct gaaagacctg
     2341 ttcaaaacct ccaacggcaa atacattgct ccgcaggaga tagaaacccg cgtaagcacc
     2401 gataaattta tcgaacaggt agccgtgata ggcgatgaac gaaattatgt gaccgccatc
     2461 atcgctcctt ccatccccgc actggaaaaa tacgccaaag aacacaaaat cgtttatcag
     2521 gatgtggacg aattgctgcg gcttccagaa atcaagaaac tgatagaaga caggattgcc
     2581 gagcaccagc agggaatggc aagctatgaa atgataaaaa aaatcacact catcaaaatc
     2641 ccgttcacca ttgaagccgg cgaactgacc aacacactga aaatccgccg ctcggtcatc
     2701 acccaaaaat acaaaaacga gattgatgcg atgtatgagg cgtagcaggc ttagtagttt
     2761 ctgtagccaa tgtgcaatgt gctaatttgt caatatgcta atgtgctaat gtgccaatat
     2821 gccaatatgt gaatgtgcca atgagccaat aaactaatat acgaataaac taattcacca
     2881 atacaccaat acaccaataa accaataaac caataaacca ataaactaat acactaatac
     2941 accaataaac ctcaaaaaaa atcttgtcac gtggcaaaaa aatagtaatt ttgcagcgaa
     3001 aacagaaaac gtaaaataaa cattctaaat aaatacaatt atggtaagtt acaaagagtt
     3061 aggcctggta aactccaaag agttgttccg gaaagccatc gaaggaaaat atgcaattcc
     3121 tgcattcaat ttcaacaacc tggaacagtt gcaggcaatt gtttcggcct gtgttgaaac
     3181 aaaatcgccg gtgatacttc aggtatccag cggagcacgc aagtatgcca atcagacact
     3241 gctgcgctac atggctcagg gagcagttca atacgccaaa gagctgggtt atgaaattcc
     3301 aatcgttctt cacctggacc acggagatag ttttgaacta tgcaaagact gtatcgaaag
     3361 cggtttttca tcggtgatga tcgacggatc gcacctgcct tatgaagaaa acattgccct
     3421 gaccaaaaaa gtggttgaat ttgctcatgc tcacgatgta accgtagaag gtgaactcgg
     3481 cgtgctggca ggtatcgagg acgatgtggt agccgaacac cacacttata ccgaacccga
     3541 acaggtagta gatttcgtat cgcgcaccgg tgttgattca ctggctattt ctatcggcac
     3601 ttcacacgga gccaacaaat tcacaccggc acaatgcacc cgcgacgaaa atggcgttct
     3661 gattccaccg ccgcttcgtt tcgatatcct cgaagaaatt gaaaaacaaa tccccggatt
     3721 ccccatcgta ttgcacggtt catcttccgt tccacaggaa tatgtagcca ccattaacaa
     3781 attcggtggt gcgctgaaag attctatcgg aattcccgaa gaacaacttc gtcatgctgc
     3841 cgaatcggct gtttgcaaaa tcaacattga ctccgacggc cgtctggcta tgacagctgc
     3901 cgtacgcgaa gtatttgcaa ccaaaccggg cgaatttgac ccgcgtaaat acctcggacc
     3961 agcccgcgac gaactgaaaa aactttacat gcacaaaacc caatcggtat tgggcagtgc
     4021 aggaaaagca taaattctgc cccgaaagag agttctgaag acagaaactg caaaacaaca
     4081 aaaatcccaa tccttccgaa tcaatttcag tgatggattg ggttttttat ttgatgtcac
     4141 tgcgattgcc acatcatttg tgacgtgaat atgaattata atcgcatgat aacctattat
     4201 cagcaagtga aacgggtgga cagagttcca actctgtcaa taaactctct gcattcaaat
     4261 cattccgaca gaatcggaat tagcttcgct gaacgttgtt tctgtccgcc cgtggcagca
     4321 ttattatcgg aattccagcg cctgttaaca ttatcataca gcactaccca aaagtcccat
     4381 aaacagaaat aatccgcttc cttccccttg tgggggaagt cccgtagggg atgggggctg
     4441 ggattaaggg gccgatccca ttattatttc gggagagctg ttcatcatga gattcctcac
     4501 tgcgaaatcc ttcacttcgt tccggttttc ttgttcggaa tgacaacgtt acttttttta
     4561 ttacccgtcg aaaatattta tgaaaataat gtgatttcaa cacgacgggg cgtcggtagt
     4621 tttgcaccga aagagttaca agtaaccaat agaataaaac cagaaaatga atctacgttg
     4681 cattacacaa ctctccccct tcgggggagt tggaggggac ttttcaccca aaagtcctat
     4741 aaacaaaaaa aatctgcttc tttccccttg tggtcactga gctggtcgaa gtgaggggga
     4801 gtcccaaagg ggatgggggc cgggatttag ggggctgtcc agtcccgatg gctatcgggt
     4861 tagggagggt tttgccaact gcttcgtgcg gtttcacttg cagggattta gatgttagca
     4921 tatttttcag gcaacacgca gatatatatt tgaaactagg ggctgcagcc ccttgtttcg
     4981 ataaatttat ccggagcaat gccggtagcc ggatcagccc cttgtttgaa tatttttatc
     5041 cggagcaata cgacaggata gagtacgtca tagtattttc atacaggata tgaattacaa
     5101 aatacccgtg attctgataa atcccttaag taatttgtga tcatttataa aaaaaaacct
     5161 gacaagtatg gatacttatc aggttttgta aattaatcag atggtgactc ccgctggaat
     5221 ttcccgggag tctttccttt ttttaagtcg ggtcgattgc tccgagagga agtaagagag
     5281 gggtactttt acggtactat caccttccct acccggtttc cgactttgac tatgagtact
     5341 cccgaaattt gtacaggcag tatgttcaac ccttcggaag cttttgaaat cagcattttc
     5401 tgaccgctaa gattgaata
//
LOCUS       DAORKP010000139         5401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3298_length_5401_cov_9.477740, whole genome shotgun sequence.
ACCESSION   DAORKP010000139 DAORKP010000000
VERSION     DAORKP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_5401_cov_9.477740"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLN75_07725"
     CDS             complement(<1..708)
                     /locus_tag="PLN75_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRG04044.1"
                     /translation="MQDSIYHTIDSVNHTRVDSAFLSDSIHRADSLSLIDSVKMDAPV
                     FSGFEGILHPSSPSSESWVFIILVLLFLLLVIGIIRSAGEFFQNIKIFFSKKEPVNII
                     QTETVNFAQFQIIITIFTISVFTLTVYEVFFISSSGFHLKTFGLLSGITAGYYLLKRI
                     LINMIGYTFFKKKITNIYNKMYFNLMNVLAVVLFPVLVLFTYQPGSWHQPLLISMAGL
                     TGIFYIFLIVKLFQVFYS"
     gene            complement(735..1313)
                     /locus_tag="PLN75_07730"
     CDS             complement(735..1313)
                     /locus_tag="PLN75_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRG04045.1"
                     /translation="MKNICVYCASSSKIDSKYVDSAKKLGHLLAENSMQLVYGGGSVG
                     LMGILADSVLEKGGKVTGIIPQFMVEAEWHHEGLTELVLTETMHERKEKMASMSDAAV
                     ALPGGCGTLEELLEVITWKQLGIYKNPIIIVNVDGYYNHLLAQFRQAADELFMRPQHL
                     DMWTVVETADQVIEAIRNSKPVHEDLRQYAKI"
     gene            1384..2388
                     /locus_tag="PLN75_07735"
     CDS             1384..2388
                     /locus_tag="PLN75_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRG04046.1"
                     /translation="MILVTGATGMLGGHLIRELLRENSEVAAIKRKSSETQSLKSIFG
                     FYGDDVEDLYNRITWIEADVEDYYALCKAFEGIKYVYHCAARVNLGKNDNKMFEVNVG
                     GTKNVVDAALINKVRKLCFVSSIAALSDGNGQNEIDEETPAVHNNTNTIYGESKRLAE
                     LEIKKGIDKGLDSVIVNPGVILGYSKEMKGSGVLFERVKKGMPFYTNGLTGYVAVQDV
                     AKAMILLMKSDISDERYVLTAENCLHKDVLRWMAEGYDVMRPFIGMNRSLPVIACMLE
                     FLGKLFGFTPIIDRSSARVAISKKSYSSQKFLSVFPDFKFSDIRDTIIQIGEFDKNAT
                     "
     gene            complement(2466..3464)
                     /locus_tag="PLN75_07740"
     CDS             complement(2466..3464)
                     /locus_tag="PLN75_07740"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRG04047.1"
                     /translation="MTALYTLKNGKGMSAEICNYGARVTKLNVPDKNGMTADVVLSFD
                     TLEEITTKETYFNSTCGRFANRIKDAKFTLEGKEYKLAVNNGPNSLHGGAEGFNMKVW
                     DVKSVTESEIILHYLSPDGEEGYPGNLDITLTYQLTDENELKIHYEAICDKTTVIGLT
                     NHSYFNLKGAGEGLIHDHFLQLNADFHTALDEFTCPTGEILKVDNSPYDFRHPVLLGE
                     RINDPVYASNRGLDDNWVIRKNQPGELALAGYIYEPESGRKMEVITTQPGVQVYTANW
                     VDKQTAKYGKEYNAQGAICLETQGFPASPNFSHFPSTVLRAGEKYDEWCIYKFSAE"
     gene            complement(3583..4746)
                     /locus_tag="PLN75_07745"
     CDS             complement(3583..4746)
                     /locus_tag="PLN75_07745"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRG04048.1"
                     /translation="MEISELKNAFKEAYGVDADAVYFSPGRVNLIGEHTDYNGGFVFP
                     CALSFGTYLLLRKNGEKFVQFRSLNLPEVVKIPLDKLTERLENNSWANYPLGVFAQFV
                     KRGYNIDQGFDIMIWGNVPNGAGLSSSAALETVTAFALNDQLGTQLGRTELAKIGQKA
                     EHEFALVNCGIMDQFASAQGKKDHAIFLNCDTLDFELVPVKLEGVKVVISNTHSPHKL
                     DSGAYNSRVAQCQLAVKQINEVRPIKYLAELTEEEFKKIESAITDEVAHKRARHVVGE
                     VQRTTDAVKALKEGNLKLFGELMNASHVSLRDDYEVTGPELDTMAEEAWKIDGVIGSR
                     MTGGGFGGCTVSLVKDEAIDEFIEKVGAAYEKKIGIKPDFYIAEIGDGAKKLD"
     gene            complement(4872..5288)
                     /locus_tag="PLN75_07750"
     CDS             complement(4872..5288)
                     /locus_tag="PLN75_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04049.1"
                     /translation="MKKAIWIFILVVLAGLSGLIYYKYFFVFGEGVKAGELNYMVRKG
                     VVFKTYEGKLIQSGFRSSSGKTAGTGIQSYEFEFSVIDKAIADSLMLCSGRDMELHYK
                     EYKGTLPWRGFSRYVVDKIISIDESRYPKTQISPGN"
BASE COUNT         1541 a         1055 c         1117 g         1688 t
ORIGIN      
        1 agaataaaat acttgaaata acttcactat taagaatata taaaatatgc ctgtcaaacc
       61 tgccattgat attaaaagtg gctgatgcca gcttccgggc tgataggtga aaagaaccaa
      121 aacaggaaaa agtactacag ccaatacatt catcagatta aaatacattt tgttatagat
      181 atttgtaatt ttctttttga agaatgtata gcctatcata tttatcaata tgcgctttaa
      241 caggtagtat ccagccgtta ttccggaaag caaaccaaat gttttgagat gaaatccgga
      301 agatgatatg aaaaacactt cgtatacagt aagtgtaaaa acgctgatgg taaaaatggt
      361 gatgataatc tgaaattgtg caaaattaac agtctccgtc tgaatgatgt ttaccggttc
      421 cttttttgag aaaaatattt tgatgttttg aaaaaactct cccgccgacc ggataatacc
      481 aataaccaga agcagaaaaa gtaaaacaag tataataaaa acccagcttt cggaggatgg
      541 agaggaaggg tgaagtatac cttcaaatcc cgaaaaaaca ggggcatcca tttttacaga
      601 atcaatgagc gacagggagt cagcccggtg aatactgtct gacaggaaag ccgaatcaac
      661 tctcgtgtga tttacactgt ctatagtatg atatatgctg tcttgcatat ctctctgttt
      721 atcacttttt gggattatat ttttgcatac tgtctcaggt cttcgtgtac aggtttgctg
      781 tttctgattg cctctatcac ctgatcggct gtttcaacca cagtccacat gtctaaatgt
      841 tgcggacgca taaaaagctc atcggctgcc tgacgaaatt gcgccagcaa atggttgtaa
      901 taaccatcaa cattgacgat gataatcgga tttttatata taccaagttg tttccaggtg
      961 atcacttcca gcaattcttc cagtgtaccg catccaccgg gcaaagctac agcagcatca
     1021 ctcatggaag ccattttttc cttacgctca tgcatggttt ctgtcagaac aagttcggtg
     1081 agtccttcgt gatgccattc ggcttctacc atgaattgtg gaataatgcc ggtgactttg
     1141 cctccttttt ccaatacact atcagccagt attcccatca aacctacaga acctcctccg
     1201 tatacaagct gcatactgtt ttcagccagt aagtgaccaa gttttttagc tgaatcgacg
     1261 tattttgaat ctattttact acttgaggca cagtatacac aaatattctt cataaccttg
     1321 aaattttgtg caaaattacg gttttctttc cgtatttttg agacttcaat caattttacg
     1381 ataatgattt tagtaacagg tgcaaccgga atgcttggtg gacatttaat ccgcgaattg
     1441 ctccgtgaaa acagcgaagt ggctgctata aagcgtaaat cctctgaaac gcaatctctg
     1501 aaaagtattt tcggatttta cggtgatgat gtggaagatc tctacaatcg aatcacatgg
     1561 attgaggctg atgttgaaga ttattacgcc ttgtgcaaag cctttgaagg aattaaatat
     1621 gtgtatcact gtgcagcaag ggtgaatttg ggcaagaatg acaataaaat gttcgaagtc
     1681 aacgtaggcg ggacaaaaaa cgttgtagat gcagcgttga ttaacaaagt cagaaaactt
     1741 tgttttgtga gttccatagc agctttgtcc gatggaaacg gacaaaatga aatagatgaa
     1801 gaaactcccg ctgtgcataa caatacaaat acaatttatg gcgaaagtaa gcgtcttgcg
     1861 gaattagaga taaaaaaagg aattgataaa ggtttagatt cagtaattgt taaccccggt
     1921 gttattttgg gatattcgaa agaaatgaaa ggaagcggtg tgcttttcga acgggtgaaa
     1981 aagggaatgc ctttttacac aaatggactt acgggatacg tagccgtgca ggatgtggca
     2041 aaagcgatga tacttttgat gaagagtgat atcagtgacg agcggtatgt attgacagct
     2101 gagaactgtt tgcacaagga tgtgcttagg tggatggccg agggttatga tgtgatgcgt
     2161 ccgtttatag gaatgaaccg gtctttgccg gtaattgcct gcatgttaga gtttttaggt
     2221 aagctgtttg gttttacgcc gattatcgac aggtcatctg cacgggtagc tatcagtaaa
     2281 aaatcatatt cttcacagaa atttttgtcg gtttttccag atttcaaatt ttcggacatt
     2341 cgggatacaa tcattcagat aggtgaattt gacaaaaatg caacctgatt ttttctaaaa
     2401 agaaacgact ttacaaaaaa gtgttgtaaa gtcgttcagg tattaaaaca aaacaaaata
     2461 tttttttact cagcagagaa tttataaata caccattcgt cgtatttctc gcctgcacgc
     2521 agcacggtag atgggaaatg tgaaaaattg ggtgatgcgg ggaatccctg agtctcgagg
     2581 cagatggctc cctgcgcgtt gtattctttt ccgtacttag cggtttgctt gtccacccag
     2641 ttggcagtat aaacctgtac tccgggttgt gtggttatca cttccatttt tcgtccgctt
     2701 tccggttcgt aaatatagcc ggctaaagcc agttcaccgg gttgattttt gcgtattacc
     2761 cagttgtcgt ccaatccgcg gtttgaagca tatacaggat catttattct ttcgccgaga
     2821 agcaccggat gccgaaaatc ataaggtgaa ttgtcaactt tcaaaatttc accggtaggg
     2881 caggtgaact catccagcgc agtgtgaaaa tcggcattta actgtagaaa atggtcgtga
     2941 atgagccctt cgcccgctcc tttgagattg aaataggagt ggttggtcag tccgattact
     3001 gtggttttat cgcagattgc ttcgtagtga attttcagtt cgttttcatc tgtgagttga
     3061 taggtaaggg taatatccag attgccggga tagccttctt cgccatcggg cgaaaggtaa
     3121 tgaagtataa tttcactttc agtgaccgat ttcacatccc atactttcat gttgaatccc
     3181 tctgcaccac cgtgtaatga atttggtccg ttgtttacgg ccagtttata ttctttacct
     3241 tcgagtgtga attttgcatc ttttatgcgg ttggcgaatc gtccgcaggt agagttgaaa
     3301 taggtttctt tagtagtgat ttcttcgagc gtatcgaaac tcaacacaac atctgcagtc
     3361 attccgtttt tatccggaac gttcagtttt gtcacccggg caccgtagtt gcatatttcg
     3421 gctgacatgc ctttgccgtt ttttaaagta taaagtgctg tcattttttg ttgaaaacca
     3481 ctcttatccc ctgatgaggc aataagagtg ggttgattga aaagttaatt tttttcttaa
     3541 tgtaaaaaaa atacggagtt gagatttaat ctttcttcgt gtttaatcca gtttttttgc
     3601 tccgtcgcct atttcagcaa tgtagaagtc cggtttgata ccaattttct tttcataggc
     3661 agcgccaact ttttcgataa attcatcgat ggcttcgtct ttaaccaatg aaacagtaca
     3721 tccgccaaaa ccgccaccgg tcatgcgtga gcctattacg ccgtctattt tccatgcttc
     3781 ttctgccatt gtgtccagtt cgggcccggt aacttcatag tcatcgcgca gcgaaacgtg
     3841 tgaagcattc atcagttcac caaacagctt cagattgcct tctttcagtg ctttcacagc
     3901 atcggtagtg cgctgaactt cacctaccac gtggcgggcg cgtttgtgag ccacttcgtc
     3961 ggtgatggca ctttctattt ttttgaattc ttcttcggtc agttctgcca ggtattttat
     4021 cggacgaacc tcgttgattt gttttacggc cagctgacat tgggctacgc gtgaattgta
     4081 agctcctgaa tctaatttgt gtgggctgtg tgtgttggaa atgaccactt tcacaccttc
     4141 cagttttacg ggaacaagtt caaaatcaag ggtatcgcaa ttgaggaaaa tagcgtgatc
     4201 ttttttgccc tgtgcacttg caaactgatc catgatgccg cagttaacca gcgcaaattc
     4261 atgttcggct ttctgcccta tttttgcgag ttcagtacgg cccagttgag ttccgagctg
     4321 atcattcagt gcaaaagcgg taacagtttc gagtgctgca gatgatgaaa gtccggctcc
     4381 attgggtaca ttaccccaaa tcatgatatc aaaaccctgg tcgatattgt agccgcgttt
     4441 tacaaattga gcaaaaacgc caagcggata attagcccat gaattgttct caagtcgttc
     4501 ggtgagttta tccaacggga ttttaaccac ttcaggcaaa ttgagtgagc ggaattgcac
     4561 aaacttttcg ccgttctttc ttagcagcag gtatgtaccg aaacttaaag cacacgggaa
     4621 tacaaatcca ccgttgtaat cagtgtgttc tccaatcagg ttaacccggc cgggtgaaaa
     4681 ataaacggca tcggcatcta ctccgtaagc ctctttaaaa gcgtttttta attcagaaat
     4741 ttccatattg tattaaattt tataatgaat aattcaagat aaaggcgata aaatcataca
     4801 ttttacctta tgcaaatgta tgataaaata gtagaaattt ttgttaacag tttatgcgta
     4861 tttgttaatt ttcaattacc cggagaaatt tgtgttttcg gatatctgct ttcatctatg
     4921 gagatgattt tatctacaac atatctgctg aaacctctcc agggcagggt tcctttatat
     4981 tctttgtagt gtagctccat atcccggcca ctgcaaagca tcagcgagtc agctatggcc
     5041 ttatctatta ccgaaaattc aaattcgtaa gactggatgc ctgttccggc agtttttccc
     5101 gaagatgatc gaaatcccga ctgtataagt tttccttcgt aagttttgaa aacaactcct
     5161 tttcttacca tgtaattaag ctcgccggct tttacacctt ctccaaatac aaaaaaatac
     5221 ttgtaataaa ttagccccga gagtcctgcc agaactacca gaatgaatat ccaaatcgct
     5281 tttttcatgt tttaaaaaat ttttaatagt gtaatgctga gatattaaat gcaaatataa
     5341 tgtttttttt agtacatgac aaaaattata tagcgatata attatttgat aatcagcaaa
     5401 a
//
LOCUS       DAORKP010000140         5398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3304_length_5398_cov_9.000187, whole genome shotgun sequence.
ACCESSION   DAORKP010000140 DAORKP010000000
VERSION     DAORKP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3304_length_5398_cov_9.000187"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLN75_07755"
     CDS             <1..371
                     /locus_tag="PLN75_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HRG04050.1"
                     /translation="DVSKLPFDHHELMAMVAPRALLCLGNPDFEWLADESGYVSCKAA
                     KRVWETFGIADRFGYSIAGGHGHCQLPEFQRPEVEAFIDKFLLGKKNVDTNVEKHPYP
                     NVDFERWTNWWGTKNPVFQK"
     gene            384..2867
                     /locus_tag="PLN75_07760"
     CDS             384..2867
                     /locus_tag="PLN75_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HRG04051.1"
                     /translation="MNKLKLHIILFLLSVAAMLSAQNAPTFISYSGDTGNWELTDTSK
                     PISILMDENEYSGVQNAILHLQNDIEMVTGKKSVVLNSGNQTIKIIVGTYNKNKLINK
                     LVGKVDLEGKYEKFIIQTIENPADGVEKALVIAGSDMRGTIYGIYELSRQMGVSPWYY
                     WADVPVRKSEKIFIKPGKFTAGEPAVKYRGIFLNDEAPALSGWSKATFGGFNHLFYEK
                     VFELILRLKGNFIWPAMWGNAFYDDDPENGPLADKMGIVIGTSHHEPMGRAHAEWSKY
                     GTGKWDYSKNQQVLAEFWRSGFERMKNYETVVTLGMRGDGDEPMSEESNIKLLEKIVK
                     EQRNIIKKVSGKKITETPQVWALYKEVQDYYDKGMRVPDDVTLLFCDDNWGNVRKLPD
                     PAAKAHKGGYGMYYHFDYVGGPRNYKWINVSQIQRIWEQMNLTYSHGVDRIWIVNVGD
                     LKPMEYPISFFLDMAWNPAQFNSQNLLSHTEKWCAAQFGEKYSKEAARLIDTYTKYSR
                     RITPELLNDTIYSLKNYNEFETVMNDFRALALDAFRLYQQIPAESRDAFDQLVLFPIN
                     AFSNLYEMYFAVAKNKELAKQNNPKANFWADKVKACFDRDSLLTVHYNTVISGGKWAH
                     MMDQVRIGYTYWQQPERSVMPAVVRVEPTNKGIFTEKDGYISMEAANFSRKNESANVR
                     WEIIPNLGKTLSGITTFPQNYYPNSNENIYLEYDFQLESEGEFPVYILVSPTLNFNEN
                     KGLRYAVSVDGQQEQIVNINDKYDVRKMERWQAKSINQTITKHNFNKKGKHTLRFRVL
                     EPGIVLQKIMIDTGGLKPSYLGAPESIKR"
     gene            2969..4375
                     /locus_tag="PLN75_07765"
     CDS             2969..4375
                     /locus_tag="PLN75_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG04052.1"
                     /translation="MELNTMTKGESKGFYKLSWVQRIGFGSGDLAQNLIYQTVAQYLL
                     IFYTNVFGLPAATAAVMFLIVRLIDVVWDPIVGTFVDKRNPKIGKYRSYLVLGGIPLT
                     GFAILCFWNGFSGSLLYAYITYVGLSMCYTLVNVPYGALNASLTRDTDEITKLTSVRM
                     FLANLGGLAVAYGIPIIVKSLSPDGKINSPESGKAWFITMTIYALAGLALLIFSFTQT
                     KERVVMDDKDMEHVKVSDLWTEFKRNRPLRILAFFFITAFAMMAIGNSAGSYYMIYNV
                     HAPDMLPYFAALGSIPAFIFMPMVPAIKRAIGKKQMFYVFLSIAIFGMFILYIVSVIP
                     ALKTQVWLVLTAQFIKSTGVIVATGYMWALVPEVISYGEHTTGRRISGIVNALTGIFF
                     KAGMALGGVVPGFVLAMVKFDEKNTVSQSPLVQQGILWLVAVIPALLLMLAMFTISKY
                     ELEDDVIDKINMEIEQRQ"
     gene            4401..>5398
                     /locus_tag="PLN75_07770"
     CDS             4401..>5398
                     /locus_tag="PLN75_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRG04053.1"
                     /translation="MKTKTQKATIFSILTLLFVALTGCANHKVTLKDALKDKFLIGVA
                     MNEAQITEADSSSVAIIKNHFNSITAENCMKSGEIQPEEGKFNFDLPDKFVKFGEENN
                     MYIIGHTLVWHSQAPGWFFTDKAGKDVSREVLIERMKNHIFTVVGRYKGKVKGWDVVN
                     EAIEDDGSWRKTKFYNIIGEDYIKLAFQFAHEADPDADLYYNDYSMAHEGRRNAVVKM
                     VNNLKSQGVKIDGVGMQGHMDMVFPDLDEFEKSMLAFAETGVKLMITELDVTVLPRPG
                     IDVGAEISASFEYQQKLNPYAEGLPDSVSIALNNRYNDIFKLFLKHSDILERVTLWG"
BASE COUNT         1657 a         1060 c         1250 g         1431 t
ORIGIN      
        1 aagatgtatc gaaattgccg tttgatcatc acgaactgat ggcaatggtg gctccgagag
       61 cgcttctctg cttgggaaat cccgattttg aatggctggc ggatgaatcg ggttatgtgt
      121 cctgcaaagc tgcaaaacgt gtttgggaaa ctttcggaat tgccgacaga tttggatatt
      181 ccatcgccgg aggtcatgga cattgtcagt tgccggaatt tcaacgacct gaagtagagg
      241 cttttatcga taaattttta cttggtaaaa aaaatgtaga tacgaacgtg gaaaaacatc
      301 cttatcctaa cgttgatttt gagcgttgga caaactggtg gggaacgaag aatcccgttt
      361 ttcaaaaata accgaatata aaaatgaaca aactgaaatt acatattatc ctttttctac
      421 tttctgtcgc agcaatgctg tccgctcaaa atgcaccgac ttttatttcc tactccggtg
      481 ataccggaaa ctgggaattg accgatacaa gtaaaccgat ttcaatttta atggatgaaa
      541 atgagtattc cggagttcag aatgccattt tgcatcttca gaatgacata gaaatggtta
      601 ccggtaaaaa atcggtagta ctgaattcgg gcaatcaaac gattaaaatc attgtaggta
      661 cttacaacaa aaacaagctt atcaataagt tggttggaaa agtcgatctg gaaggaaaat
      721 acgaaaaatt cattattcag actattgaaa atcctgcaga tggagtcgaa aaagctctgg
      781 tcattgccgg aagcgatatg cggggtacca tttacgggat ttatgaactt tcccgtcaga
      841 tgggagtttc accctggtat tactgggcag atgttccggt gagaaaatcg gaaaaaatat
      901 tcataaaacc gggaaaattt accgccggag agcctgccgt gaaatatcgc ggcattttcc
      961 tgaacgatga agcaccggct ttgtccggat ggtcgaaagc cactttcgga gggtttaatc
     1021 atttgtttta cgaaaaagtg tttgaactga ttctccggct gaaaggaaat tttatctggc
     1081 ctgccatgtg gggaaatgct ttttacgacg atgatccgga aaacggaccg cttgcggata
     1141 agatgggcat cgttatcgga acctcgcacc acgaaccgat ggggcgggca catgccgaat
     1201 ggagcaaata tggaacagga aaatgggatt acagcaaaaa ccagcaagtg ttggctgaat
     1261 tctggcggtc gggtttcgaa cggatgaaaa attacgaaac ggtggttacg ctcggcatgc
     1321 gtggcgacgg cgacgaaccg atgagcgaag aaagcaacat caaactgctc gaaaaaatcg
     1381 tgaaagaaca gcggaatatc atcaaaaaag tttcaggcaa aaaaatcacg gaaacgccgc
     1441 aggtctgggc actctacaag gaagtgcagg attattacga taaaggaatg cgcgtgccgg
     1501 acgatgttac attgctgttt tgcgacgaca actggggaaa cgtgcgcaaa ctgcccgatc
     1561 cggcagcaaa agcacacaaa ggcggatatg gaatgtatta tcacttcgac tacgtgggcg
     1621 gtccgcgcaa ttacaaatgg atcaacgtga gccagataca gcgcatctgg gaacaaatga
     1681 acctcactta cagccatgga gtagaccgaa tctggattgt aaatgtgggc gatttgaaac
     1741 cgatggaata tcccataagc ttcttcctgg atatggcctg gaatccggct cagttcaatt
     1801 cacaaaatct gctcagccac accgaaaaat ggtgtgccgc acaatttgga gaaaaatact
     1861 cgaaagaagc agcccgactg atagacactt acaccaaata cagccgcagg attacacctg
     1921 aactgctgaa cgataccatc tacagcctga aaaattacaa cgaattcgaa accgtaatga
     1981 atgatttcag ggcgctggcg cttgacgcat tccggcttta tcagcagatt ccggcagaga
     2041 gcagagatgc ttttgatcag ctggttcttt ttccgataaa cgcattttca aacctgtatg
     2101 aaatgtattt tgcggtagcc aaaaacaagg aattggcaaa acaaaacaac cccaaagcaa
     2161 acttctgggc agataaagta aaggcttgtt ttgaccggga ttcgctgttg actgttcatt
     2221 acaatacagt gatttcaggt ggaaaatggg cgcacatgat ggatcaggtg cggattggct
     2281 acacctactg gcagcagccc gaacgaagcg tgatgcctgc cgtagtgcgg gttgaaccga
     2341 cgaataaagg catttttacg gaaaaagacg gttatatttc catggaggca gcaaattttt
     2401 ccagaaaaaa tgaatcggcg aatgttcgat gggaaattat cccaaatctg ggaaaaaccc
     2461 tttccggaat cactactttt ccacaaaatt attatccaaa ttcgaacgaa aatatttact
     2521 tggaatatga tttccaactt gaatcggaag gagaatttcc agtttatatc ctggtatcgc
     2581 caactcttaa tttcaacgaa aacaaaggcc ttcgctacgc cgtttcggtg gatggacagc
     2641 aagagcagat cgtgaatatt aacgacaaat acgatgtgag aaaaatggaa agatggcagg
     2701 caaaaagcat taatcaaacc attacaaaac ataatttcaa caaaaaagga aaacatacgc
     2761 tccgcttccg ggtattggaa ccgggcattg ttttgcaaaa aataatgata gatacaggcg
     2821 gattgaaacc gagctactta ggcgctccgg agagtataaa aaggtgattc aacaataaac
     2881 cagaattctg cttttattcg gaaataatca gattaattga aaaaataagt gcaatcgtag
     2941 aaaaaataaa ctaaaactta tcaaataaat ggaactaaac acaatgacaa aaggtgaatc
     3001 gaaaggattt tataaacttt cgtgggtgca acgtatcggt ttcggatcgg gcgacttggc
     3061 acaaaatctt atttatcaga cagttgcgca atacttgctc attttttaca cgaatgtttt
     3121 tggacttccg gccgctacgg cagctgtgat gtttctgatt gtgaggttga tagatgtcgt
     3181 ttgggatcct attgtaggaa catttgtaga taagcgaaat ccgaaaatag gcaaataccg
     3241 ttcctatctg gtgttgggcg gaataccgct tacagggttc gccatccttt gtttctggaa
     3301 cggattttcc ggttcattgc tttacgctta catcacctat gtaggtttat ccatgtgtta
     3361 cacgctggta aatgtgcctt acggtgccct caatgcatcg cttacgcgcg ataccgacga
     3421 aatcactaaa ctcacttcgg tgcggatgtt cctggccaat ttgggtggtt tggccgttgc
     3481 ttacggcatt ccgattattg taaaatcact ctcgcccgat ggaaaaatca actcaccgga
     3541 atcgggtaaa gcctggttta tcaccatgac aatttatgcg ctggcaggtc tggcgttatt
     3601 gattttcagt tttacccaga ctaaagagcg ggtggtgatg gacgataaag atatggaaca
     3661 tgtgaaggtt tcagacctgt ggacagaatt caaacgcaat cgtccgctgc gaatcctcgc
     3721 tttctttttt atcacggcat ttgctatgat ggctatcgga aactctgccg gctcttacta
     3781 catgatttac aacgtacatg cgcctgatat gctgccttat tttgctgctc tcggatctat
     3841 tcctgcgttt atttttatgc cgatggttcc ggctatcaaa agggcaatcg ggaaaaaaca
     3901 aatgttctac gttttcttgt caatagccat tttcggtatg ttcattctct acattgtatc
     3961 agttatcccc gcacttaaaa cacaggtatg gcttgtgctt acagctcagt ttataaaatc
     4021 taccggagtg attgtagcta caggatatat gtgggcgctg gttcctgaag ttatttcata
     4081 cggcgagcat actaccggca gacgtatttc aggcattgta aacgcactca caggtatttt
     4141 tttcaaagca ggaatggcgc tgggcggtgt tgtgccgggt tttgtgctgg caatggttaa
     4201 atttgacgaa aaaaataccg tctcgcaatc tcctttggta caacagggca ttctttggct
     4261 tgtagctgtg attcccgcat tgttacttat gctggccatg ttcaccattt caaaatatga
     4321 actggaagat gacgtgattg ataaaattaa catggaaatc gaacagagac aataatatga
     4381 atcaattaaa taataagatt atgaaaacaa aaacacaaaa agcaacaatt ttttctatcc
     4441 tgacgctttt attcgttgct ttgacgggat gtgctaatca caaagttact ttgaaggatg
     4501 cgctgaaaga caaatttctg attggagtgg caatgaatga ggctcagatt accgaagctg
     4561 attcgagtag tgttgccatt atcaaaaatc actttaattc tattacagcc gaaaactgca
     4621 tgaaaagcgg cgaaattcaa cccgaagaag gtaaatttaa ctttgatctg cccgacaaat
     4681 ttgtaaagtt cggagaagaa aataacatgt acatcatcgg tcatacgctg gtgtggcatt
     4741 cgcaggctcc gggatggttt ttcaccgaca aagcaggaaa agatgtttca cgcgaagtgt
     4801 tgattgaaag aatgaaaaac catattttca cggttgtggg gcgctacaaa ggcaaagtaa
     4861 aaggctggga cgtagtgaat gaagcgattg aggacgatgg ttcttggaga aaaaccaaat
     4921 tttacaacat catcggcgaa gattatatca agttggcgtt ccagtttgca catgaagcag
     4981 atcccgatgc ggacttgtat tacaacgact attccatggc tcacgaaggc cgccgaaatg
     5041 ctgtggtaaa aatggtaaac aacctgaaaa gccagggtgt aaaaattgac ggcgttggta
     5101 tgcagggaca tatggatatg gttttccctg atttggatga attcgaaaaa agcatgcttg
     5161 cttttgctga aaccggcgtg aaactgatga ttaccgaact cgatgttacc gttcttccaa
     5221 gaccgggtat agatgttggt gccgaaattt cggccagctt cgaatatcag caaaaactta
     5281 atccatacgc cgaagggctt ccggattctg tttcaatagc tttgaataat cgttacaatg
     5341 atattttcaa attattcctg aaacacagcg atatattaga acgtgtaacg ctctgggg
//
LOCUS       DAORKP010000141         5394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3310_length_5394_cov_9.658738, whole genome shotgun sequence.
ACCESSION   DAORKP010000141 DAORKP010000000
VERSION     DAORKP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_5394_cov_9.658738"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1009
                     /locus_tag="PLN75_07775"
     CDS             290..1009
                     /locus_tag="PLN75_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRG04054.1"
                     /translation="MKSFKTFGYIAILALGLFLAAYLYSVFIKPTAYGAGNTMGMMLL
                     GLVLGGLIVFLIVKLLSKKQTEITAESSHTVVESMRKVFKIVCAEGQFQEIYNYEQTK
                     KLLKIIPSTKKALVIVKAKVLIGYDFEKCKWEIDEESKKLKIVSFPEPEILSLEPDFK
                     YYYFEEDLFNFINRDDLDKIQIQGKKQVEQAALQSGLKRIAAEQMRTVLTEMVAANRW
                     SISNPEMLTLPLQTATSDQKK"
     regulatory      1068..1286
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1398..3422
                     /locus_tag="PLN75_07780"
     CDS             1398..3422
                     /locus_tag="PLN75_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRG04055.1"
                     /translation="MNLLVEKRNLIVGILLLSGSIITFSQSKTDSVTHLREVTITQKA
                     LNPEIKSATPVQILSEKKIQTLNALLLSDAVKHFPGVTIKDYGGIGGLKTISVRGLGA
                     THTAVVYDGIAVSDVQTGQIDIGRFTLDNVQSISLNSGQTDQIFQPARMFASASVLSI
                     QTQKPVFSNRNITGKISMKAGSFGLINPSFTFHGKINRRLSASFNGDWLSANGKYPYT
                     LHYGTQYTDSSSVEIRKNTDVKNIRLEGTLFAGFSEKSNGYLKAQFFNSERGLPGAVI
                     FYNDKSTSSQRLQDNSFFTQANFTHRFSNRWAAQANAKFNRSGINYTDSAWMNSAQVL
                     ENTYNQREIYGSIAVMNSVTENLSFSAASDVSLNTMEANLHNFAYPGRLTWQSSLASK
                     YATNRISLTGSLLLTQTYESVKSGVTADNQKKVSPFVSFSVKPLEEINFRIRSFYKNI
                     FRMPSFNDLYYLRTGERKLRPEDAHQINLGITYSLSKLKWLENIDFSADMYHNRIKNK
                     IVAKPSASLFEWTMTNFGTVHITGLDLHAEALIAFSEDISVETGVNYSYQHAVDMTDE
                     TNRTYGHQLPYTPFHSGSGRVNLINQWVNIAYSVIWSGVRYSNAQNYTENRLPAYSDH
                     SISLNKTFIIDNLTFRPSIEFLNIFNKNYEVIRGFPMPGRSFRINITVNI"
     gene            3443..4513
                     /locus_tag="PLN75_07785"
     CDS             3443..4513
                     /locus_tag="PLN75_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04056.1"
                     /translation="MKRVNLLILTIFAILLSACEVEDIKKKTDLPNETVKLLVLSEGS
                     FTNNNSTLAYYDLKNKTTDKDYFLTQNKRGLGETANDMIRYGSKVYIVMNGSSRIEII
                     DINTGKSIRQIPMFEGNVAKGPRQIISHNGKVYVSSFDDTVTRIDTASMTIDGSLKTG
                     FDPEGMCVVGEKLYVANSGGLEWANGYEKTVSVVDLKTFSEIQELEVGINPVHLHADN
                     QGDIYVVSNGDYGENPARFQRIDTKTNTVTDLNLNVTNFIVFQNKAYMYSYDYFTKKM
                     SVKVFDCLTEKVINEHFISDGTILNIPYSIQIDPFNNDVYMTDAIDNSVSGDVFCFDK
                     TGKLKFQVREVGINPNNIILLD"
     gene            4749..5297
                     /locus_tag="PLN75_07790"
     CDS             4749..5297
                     /locus_tag="PLN75_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HRG04057.1"
                     /translation="MLINERQNRRERLLKVAEAMATAARTAPKGKGLDIIEILTVADE
                     HIAELSAEMIKYSEKSGLKFFLRDAENILQAEAVILIGTKTKTHGLNCGYCGFPTCEE
                     KLLHEEIPCSLNSVDVGIAIGSACATAADMRVDSRVMFSAGRAAQELGWMPGCSNIYG
                     IPISCSSKNPFFDRKTSRPAGV"
BASE COUNT         1688 a         1146 c         1084 g         1476 t
ORIGIN      
        1 ttaggatttg gaaccgaaaa tttctataat ttttagtacg aacttttttc attccaacca
       61 tcggatatgt acatcctgca gattattttc tgcaggattt tccattttac acggtttttt
      121 taatgagttt gtacgtcggg gattattttt ccatttattc ttaatacatt tgtaattaat
      181 aatttaccaa ttcgctccgg ttacataagg aaacatcttt tttacagaaa aagcctaaaa
      241 aaaactttgg ttcgggtttt gtgaaagata ataatataca cttcgacaga tgaaaagttt
      301 caaaacattc ggatatattg ccatccttgc acttggctta tttctggcag cctacctgta
      361 ttctgtattt atcaaaccga ctgcctacgg cgcaggcaac accatgggaa tgatgctgtt
      421 aggccttgtt ttaggaggat taatcgtatt tctgattgta aaactactct ccaaaaaaca
      481 gacagaaatt accgccgaaa gttcacatac ggtagtggaa agtatgcgaa aagtattcaa
      541 aattgtatgc gccgaaggtc agtttcagga aatatacaac tacgaacaga caaaaaaact
      601 gctgaaaatc atcccttcaa ccaaaaaagc gctcgtcatt gtgaaggcaa aagtgctgat
      661 tgggtacgat tttgaaaaat gcaaatggga aattgacgag gagagcaaaa aactaaaaat
      721 cgtttcgttt cccgagcccg aaattctttc gctcgaacct gatttcaaat attactattt
      781 cgaagaagac ctcttcaatt tcatcaaccg cgatgatttg gacaaaattc agatccaggg
      841 caaaaaacag gtggaacagg cagctctgca aagcggattg aagagaattg ccgccgagca
      901 aatgcgcacc gtgcttaccg aaatggtagc cgccaaccgc tggtccattt ccaatccgga
      961 aatgcttaca cttccgctcc aaactgccac atccgaccaa aaaaaataaa ctgttaaatt
     1021 cgattatttc ttacctatta ttttttccca caggaaataa tctctacctt tgcatcgaaa
     1081 attttggtgt caggcattcc ttccattacc ggaatgcaag atgaaaaggg aatcaggtgc
     1141 aaatcctgaa cagacccgct gctgtaagct ccaccacaac gttctgaaca aaaacacctt
     1201 gagccactgt accgccacgt gcggaacggg aaggcgttcg gaaacgggag taagtcagaa
     1261 gacctgccaa attaatctgt taattgcttt cggggaatgg agcattaaat cgaacgtaca
     1321 atcaactttt ttcttttatt tctcagcaga tttgtgcttt ctttttggtt ctgtttcctg
     1381 aaattcattc gggaataatg aatttactgg ttgaaaaaag aaacctgata gttggtatat
     1441 tgctcttatc gggcagcatc atcacatttt cgcagtcgaa aaccgacagc gtgacacacc
     1501 tgcgcgaagt gacaatcaca cagaaagccc taaatcccga gattaaatca gcaactcctg
     1561 ttcaaattct ttcagaaaaa aaaatccaaa cgctcaacgc cctccttcta tcggatgctg
     1621 tgaaacattt cccgggtgta acgattaagg attacggcgg cattggtggt ctgaaaacca
     1681 tttcggtgcg tggtcttggt gccacccaca ctgcagtggt gtacgatgga attgctgttt
     1741 ccgatgtcca gaccggccag attgacatcg gccgttttac gctggataat gtgcaaagca
     1801 tttcgctgaa tagcgggcaa accgatcaga tttttcaacc ggcgaggatg tttgcctcag
     1861 cttcggtact cagcatccaa acgcagaaac ccgtattttc caaccggaat attacaggaa
     1921 aaatttcaat gaaagcaggt tctttcggac ttattaatcc ctcttttact tttcacggaa
     1981 aaataaatcg tcgtctgtct gcctcattca atggcgattg gctttcggcc aacggaaaat
     2041 atccttacac gctgcattac ggcacccagt acacagacag ctcttcggta gaaatacgga
     2101 aaaatacaga tgtgaaaaat atccggcttg aaggcacctt atttgccggt ttttcggaga
     2161 aaagtaacgg atatctgaaa gctcaatttt tcaattccga acggggtctg ccgggagctg
     2221 tgattttcta caacgataaa agtacctctt cacaacgact acaggacaat tcgtttttca
     2281 ctcaggcaaa tttcactcac cgtttttcaa atcggtgggc agcacaggca aatgctaaat
     2341 tcaatcgctc gggcattaac tacacagact cagcctggat gaacagtgct caggtacttg
     2401 aaaacacgta taatcagcgt gagatatatg gctcaattgc cgtaatgaat agtgttacag
     2461 agaatttatc gttttctgcc gcctcagatg tttcgctgaa tacgatggaa gccaacctgc
     2521 acaattttgc ctacccgggt cgtttgacct ggcagtcgtc acttgcttca aaatatgcga
     2581 ccaaccggat ttcgctcacc ggaagcctgc tcctcacgca aacttatgaa agtgtgaaat
     2641 cgggtgttac ggccgataat cagaaaaaag tgtctccttt tgtaagtttt tcggtaaaac
     2701 cgctcgaaga gatcaatttc agaatccggt cgttctacaa aaacatattc cggatgccct
     2761 cgttcaatga tttgtactac ctgcgcactg gcgaacgaaa actcaggcct gaagatgcac
     2821 atcaaatcaa cctgggtata acttactcat tatcaaagct aaaatggctg gaaaatatcg
     2881 atttttctgc cgatatgtat cacaaccgga tcaaaaataa aattgtagcc aaaccttctg
     2941 ccagtctttt tgaatggacg atgacgaatt ttggaaccgt tcatatcacc ggtctggatt
     3001 tgcatgctga ggcactcatt gctttcagcg aagatatttc ggttgaaacc ggcgttaatt
     3061 acagctatca gcatgccgtg gacatgacgg atgaaacaaa tcgcacctac ggtcatcaat
     3121 taccctacac gccttttcat tcgggttcgg gcagagtaaa tctgattaat cagtgggtga
     3181 acattgccta ttcggttatc tggtcgggag tacgctattc aaacgctcag aattacaccg
     3241 aaaaccggct tccagcatac tccgatcata gcatatcatt gaataaaaca ttcattatcg
     3301 ataatttaac attcaggcct tcaatagaat ttctaaacat attcaacaaa aattatgaag
     3361 taatccgcgg atttcctatg ccggggagat ctttccggat aaacataacc gtaaatattt
     3421 aatttttaaa aaatacagat tcatgaaacg agttaattta ctgattttaa cgatttttgc
     3481 cattctactg tcggcatgcg aagtggaaga tattaaaaag aaaacagatt tgccaaacga
     3541 aacggtaaag ttgctggtac tttctgaagg cagttttaca aacaacaaca gcactttggc
     3601 atattacgac ctgaaaaata aaactaccga caaggattat tttctgacac aaaacaagcg
     3661 tggactcgga gaaacagcca acgacatgat tcgctatggt tcgaaagttt acattgtaat
     3721 gaacggctca agccgtatcg aaatcatcga catcaacacc ggaaaatcca tcagacaaat
     3781 tcctatgttc gagggtaacg tggcaaaagg tccccgacag attatttcgc acaacggaaa
     3841 agtttacgtt tcatcattcg acgatactgt aacccgaatt gatactgcat cgatgactat
     3901 tgacggcagc cttaaaaccg gatttgatcc cgaaggaatg tgcgtggtag gtgaaaaatt
     3961 gtacgtggct aattcaggcg gtctggaatg ggcaaacgga tacgaaaaaa ccgtatcggt
     4021 ggttgatctg aaaacatttt ctgaaattca ggaattggaa gtaggaatca atccggtgca
     4081 cttacatgct gacaatcaag gtgatatcta cgtagtcagt aacggcgatt acggtgaaaa
     4141 tcctgccaga tttcagcgga tagatacaaa aacgaacaca gtaacagatc tcaatttgaa
     4201 tgtaaccaat ttcatcgttt tccaaaacaa ggcttacatg tacagttatg attattttac
     4261 aaaaaaaatg tcggttaagg tgttcgattg cctgaccgaa aaagtgatca acgaacactt
     4321 tatttctgac ggaactatct taaacatacc atattctatc caaatcgacc cgttcaacaa
     4381 cgatgtttac atgactgatg ccattgacaa ttcagtttcg ggcgatgtgt tttgttttga
     4441 taaaaccgga aaattaaaat ttcaggtcag agaggttggc atcaatccaa acaacataat
     4501 tttactcgat taaatccgaa ccataacagg ttatttaata tataaatcag gcaaacaggt
     4561 acagtagagc gctattgtac ctgttgtgtt aaaagctgcc cctaaccccc aaaaggggga
     4621 ataagaaatg aaatatagaa gtttgagaaa aaatatgtaa ttttgaaagc aaataatctt
     4681 caaaaatacg tataaccatt aatttaccaa cccaaagtcc cccttggggg atttaggagc
     4741 tcaatattat gctaatcaac gaaagacaaa accgccgcga gcgcctgctg aaagtggcag
     4801 aagccatggc aacagctgcc cgtacagcac ctaaaggtaa aggacttgac atcatcgaaa
     4861 tccttactgt tgcggacgag catattgcag aactatcggc tgaaatgata aaatacagcg
     4921 aaaagtccgg tttgaaattc tttcttcgcg atgctgaaaa catacttcag gcagaagcag
     4981 tgattcttat tggtacaaag accaaaaccc acgggctgaa ctgcggttat tgcggtttcc
     5041 cgacctgcga agaaaagcta ctacacgaag aaataccctg ctcactgaat agcgtggatg
     5101 tgggaattgc cattggatcg gcgtgtgcta cagctgctga tatgcgtgtg gacagccggg
     5161 tgatgttttc agccggacgg gctgctcagg aacttggctg gatgcccggt tgttccaata
     5221 tttatggaat tccgataagt tgctcctcta aaaatccatt ttttgatcgc aaaacttcaa
     5281 gacctgccgg tgtgtgaata atgaataaaa acatgatata ccctcaaata ctttcatggt
     5341 aaatatttcc gtaagtcctc tgatttttaa tgtttgtccg cattttgcct tttc
//
LOCUS       DAORKP010000142         5331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3376_length_5331_cov_9.204132, whole genome shotgun sequence.
ACCESSION   DAORKP010000142 DAORKP010000000
VERSION     DAORKP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_5331_cov_9.204132"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..648)
                     /locus_tag="PLN75_07795"
     CDS             complement(145..648)
                     /locus_tag="PLN75_07795"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRG04058.1"
                     /translation="MSKISIIAAVADNYAIGKNNKLPWHLPADLKRFRQITTGHAVVM
                     GKRTFESLPNGPLPNRKNIVLTSIPEGNYTKYYEATSLRDAIDLCDKEEEVYIIGGAS
                     LFKQALTFPLVNYMYITWVHEKFDADVFFPKFNEREWKLLQCEKHEADEKHPYPYSFC
                     EYERIKK"
     gene            complement(798..1592)
                     /locus_tag="PLN75_07800"
     CDS             complement(798..1592)
                     /locus_tag="PLN75_07800"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRG04059.1"
                     /translation="MKQYLDLLRRILDEGVRKEDRTGTGTISIFGHQMRFNLEEGFPC
                     LTTKKLHLKSIIHELLWFLSGDTNAKYLQDNGVRIWNEWADENGNLGHIYGYQWRTWP
                     DYNGGYVDQISQAVDAIRNNPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVADGRLS
                     LQLYQRSADAFLGIPFNIASYALLLQMMAQVTGLRAGDFIHTLGDAHIYLNHLEQVNL
                     QLTREPRHLPRMLINPNIYDIFGFRFEDFELTGYDPHPHIKGVVAV"
     gene            complement(1721..3727)
                     /locus_tag="PLN75_07805"
     CDS             complement(1721..3727)
                     /locus_tag="PLN75_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRG04060.1"
                     /translation="MYRLAASYLTVFILVLLTACGGTKSTLRKADQSFMKGEYALAGD
                     LYNKAYSRISSKDKPLRAETAFRQGECYRMTGYSRAEQVYMNAVRNQYADSTVFLRLA
                     QMQQKNGKIREAASNYAICLLSQPDNELALNGLKATKMIDSLKNNPTRYIISREKNFN
                     AARSSSFSPSFSGSEADVLIFTSNRKVSKKDNSLKKNTVSGQANNHLFSVKKNSQGKW
                     EKPEVLEGEVTTKNDEGIASFTSDGSTMYFTRSLTEDNRGEGVMIFISSRSGGSWGAP
                     QSISLFNDTSISVAHPAISPDGNILYFATDAPGGYGGKDLWRARLKAGKAEYVENLGP
                     EINTSGDEMFPTFRPDGTLYYSSNGKPGVGGLDIFKAVQTQKPDSTKGWQVTHMGIPL
                     NSGADDFGMAFEGDKEKGYFSSNRNETRGYDALWSFVLPELEYVLEGKITARNGSAVP
                     DARINIVGNDGESARINAKGDGSYRFRLKKDVSYQLQASARDFLNQRDSLSTYSINPR
                     ESQTFTRNFSLIPTFQSVKIDNIYYDFDKATLRPESKAGLDALIAMMNENPNITIEMS
                     AHTDYKGKDEYNRDLSARRAQSVIDYLIAAGIDAARLTAVGYGEIKPFVVDAITAQQF
                     TFLPTGTRLTQEYILTLTPDEQEICNQINRRTEFIILSTTFNMY"
     gene            complement(3830..5077)
                     /locus_tag="PLN75_07810"
     CDS             complement(3830..5077)
                     /locus_tag="PLN75_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRG04061.1"
                     /translation="MATIKPFKGIRPPKNLVEKVASRPYDVLNSDEARAEAAGNPYSL
                     YHIIKPEIDFPVGTDEHEDKVYTKAAENFAKFRSNGWLVQDENEKYYVYAQTMNGRTQ
                     YGLVVCAYVDDYMTGKIKKHELTRRDKEEDRMKHVRVNNANIEPVFFAYPHHEELDNI
                     VKDISKNTPEYDFVAEFDGFGHTFWKIDDEKTIAQITGIFAEIPSLYIADGHHRSAAA
                     ALVGNEKRLQNPNHTGKEEYNYFMAVCFPDNQLTIIDYNRLVKDLNGLTDEEFLNKLK
                     ENFQVEEKGTDIFKPESLHHFSLYLSGKWYSLIAKEGTYNDNDPIGVLDVTISSNLIL
                     DEILGIKDLRSDKRIDFVGGIRGLGELKRRVDSGEMKAALALYPVSMKQLIDIADSGN
                     IMPPKTTWFEPKLRSGLVIHDLE"
BASE COUNT         1348 a         1124 c         1214 g         1645 t
ORIGIN      
        1 gattgcaaaa atacaactat tattttaata taaaaacaaa atgaatataa aaatgcatta
       61 tttaattaca tttatctaaa aaaaagaaaa acagttcatt tttgttgaac tgttttatcc
      121 ttgtgtaaat atttattact gattttattt ttttatccgt tcgtattcac aaaatgagta
      181 ggggtaaggg tgcttttcgt cagcttcatg tttctcacat tggagtagtt tccactcccg
      241 ctcgttgaat ttcgggaaaa aaacatcggc atcaaatttt tcatgcaccc aggtgatgta
      301 catgtaattt acaagtggaa atgtgagtgc ctgcttaaag agagatgctc ctccgatgat
      361 gtacacttct tcttctttgt cgcaaaggtc aatggcatcg cgcagtgaag tggcttcgta
      421 gtatttggta taatttcctt cgggaatgga agtcagaaca atgtttttac ggttcgggag
      481 tggcccgttg ggtaaacttt cgaaagtgcg ttttcccatc actactgcat gtccggtggt
      541 tatttgcctg aatcgtttca ggtcggcagg taaatgccag ggcagcttgt tgtttttgcc
      601 gatagcataa ttgtcggcaa cggcagcaat aatcgaaatt tttgacataa ttatttgaat
      661 ttgagtaatt agctgtcaga atttttttct ctgcaaaaaa acacacaaag gtacagaaaa
      721 aaatgtggaa aaaccaaccg ttttatcttt tcagaaaagt aaatcccgat ttgatatttt
      781 ttgtcagaat tgccttatta aacagctaca acacctttga tatgcgggtg tgggtcgtag
      841 cctgtcagct cgaaatcctc aaatctgaag ccgaaaatat cgtaaatatt tggatttatt
      901 aacatcctgg ggaggtgccg gggttctctg gtgagttgta aattcacttg ttccaaatga
      961 ttcagataaa tgtgtgcatc gcccagcgta tggataaagt cacctgctct caatccggtg
     1021 acctgtgcca tcatctgaag caggagtgcg taggaggcaa tattaaaggg tattcccaga
     1081 aatgcatcgg cgctgcgctg atacaactgc agacttagcc tgccatcagc tacgtaaaac
     1141 tgaaaaaagg catgacaggg cggcagattc atgtttggca aatcggctac attccaggcg
     1201 ctcacaatga ttcttcggga atcaggatta ttgcggatgg catcaacagc ctggctgatt
     1261 tgatccacat agcctccgtt gtaatcgggc cacgtgcgcc attgatatcc gtagatgtgt
     1321 ccgaggttgc cgttttcatc tgcccattcg ttccagattc tcactccgtt gtcctgaagg
     1381 tattttgcgt ttgtgtcgcc gctcaaaaac cagagcaact catggatgat ggatttcaga
     1441 tggagttttt tggtggtcag acacgggaaa ccttcttcca gattaaaacg catctggtgc
     1501 ccgaaaatac tgatggtacc cgtacctgtg cggtcttctt tccgtactcc ctcgtcgagt
     1561 atccgccgga gcaagtctaa gtattgtttc ataggagaaa tgatgaggtg caaatgtagg
     1621 taaaaaatca ggaatgggca aaacgtagca acctgaattc cgtagccgaa ttctgtgaaa
     1681 ctggagtaaa aaaggtttta gagtaatgaa aaaaaacagg ttaatacata ttaaatgtgg
     1741 tggaaagtat gataaactct gtccgtcggt ttatctggtt gcagatttcc tgttcgtcgg
     1801 gagttaatgt gaggatatat tcctgcgtaa gccgggtgcc ggttggtaag aatgtaaact
     1861 gttgagcggt tattgcatcc accacgaatg gctttatttc gccgtagccc actgcggtga
     1921 gtctggcagc atcaatgccg gctgcaatca ggtagtcgat gaccgattgt gcacgccggg
     1981 ctgataaatc gcggttgtat tcgtcttttc ctttataatc ggtgtgagcg ctcatttcta
     2041 tggtgatatt cggattttcg ttcatcatgg ctatcaatgc atcaagaccg gcttttgatt
     2101 ctggtcgaag agtggctttg tcaaaatcgt aataaatatt gtcaattttt accgactgaa
     2161 atgtgggtat cagactgaag tttcgtgtaa atgtctggct ttccctggga ttgatgctgt
     2221 aggtcgaaag gctgtctctt tgattcagaa aatcacgtgc cgacgcctga agctggtaac
     2281 tgacatcttt tttcagcctg aaacggtagc ttccgtcgcc tttggcatta attcgggcgc
     2341 tttcaccgtc gttgcctaca atgttaattc ttgcgtcggg cactgcactg ccgttgcggg
     2401 cggtaatttt gccttcgagc acatattcca gttcgggtaa aacaaaactc cacagcgcat
     2461 cataaccgcg tgtttcgttc ctgttgctcg aaaaatatcc tttttcctta tcgccttcaa
     2521 atgccatccc gaagtcatcg gcacccgaat taagcggaat tcccatgtgt gttacctgcc
     2581 aacccttcgt actgtcgggc ttttgagtct gaacggcttt gaaaatatcg agaccgccta
     2641 caccgggttt cccgttggat gaataataca atgtgccgtc gggtctgaat gtgggaaaca
     2701 tttcatcgcc cgaagtattt atctcaggcc cgagattctc cacatattca gcttttccgg
     2761 ctttcagcct ggcgcgccac aggtcttttc caccgtatcc gcccggcgca tctgtagcga
     2821 agtaaagtat atttccatcg ggtgaaatgg ccggatgagc tactgaaatg ctggtgtcgt
     2881 tgaaaagtga gatgctctgg ggcgcgcccc acgagccacc gctgcgcgac gaaataaata
     2941 tcattactcc ttcaccccgg ttgtcttcgg taagcgaacg ggtgaaatac atggtgcttc
     3001 cgtctgacgt gaaagaggct attccttcgt cgtttttagt ggtcacctca ccctcgagca
     3061 cttcgggttt ttcccatttt ccctgactgt ttttttttac agaaaaaaga tgattgtttg
     3121 cctgtccgct tacggtgttc ttttttagcg aattatcttt tttgctcact ttacggtttg
     3181 atgtaaaaat aagtacatca gcctccgaac ccgaaaatga aggactgaac gacgaacttc
     3241 gggcggcatt gaaatttttc tcccgactga taatgtatct ggttggatta tttttaaggc
     3301 tgtcgatcat tttggtagcc ttcaatccgt ttaaagccag ctcgttgtcg ggctgtgaga
     3361 gcaggcagat ggcataattg gaggctgctt cacgtatttt accgtttttt tgctgcattt
     3421 gagccagccg caggaaaacg gtcgaatcgg catactgatt ccgtaccgca ttcatgtaaa
     3481 cctgttcggc acgtgaatat ccggtcatcc ggtagcattc tccctgcctg aaagccgttt
     3541 cggcgcgcag tggcttatct ttggacgaaa tgcgggaata ggctttgttg tataaatcac
     3601 cagccagggc atattctcct ttcataaagc tctgatctgc ttttcgcaat gtggattttg
     3661 tgccaccaca agccgtgaga agcacgagta tgaaaacggt aaggtatgaa gcggcaagtc
     3721 ggtacatata aaaataaaac ccctttatta gctgattact taaaataaac taattaaaag
     3781 ggtttttatt gtttctttag tgcggtaaaa acaccaaata gctgagagat tattccaaat
     3841 cgtgaattac aagtcccgaa cgtagtttgg gctcaaacca ggtggtttta ggaggcatga
     3901 tgttgcccga atctgcaatg tcgatcagct gcttcatcga aacgggatat agcgccagtg
     3961 ctgctttcat ctcaccgctg tccacgcggc gtttcagttc gcccagtcca cgtatgccac
     4021 ccacaaaatc aatccgcttg tcgctacgca ggtctttgat gccgagaatt tcatccagaa
     4081 tcaggttgga cgaaatggtt acatccagca ctccaatcgg atcgttgtcg ttgtaggtac
     4141 cttctttggc aatgagtgaa taccattttc ctgacagata gagtgagaaa tggtgcaggc
     4201 tttccggttt gaaaatgtca gtaccttttt cttccacttg aaaattttct ttcagcttat
     4261 taagaaattc ttcatcagtc agtccgttca aatctttcac caggcggttg tagtcaatga
     4321 tggtaagctg attatcgggg aagcacacag ccataaaata gttgtactcc tccttacctg
     4381 tgtggttcgg gttttgcagg cgtttttcat tcccaaccag tgctgcggcg gcgctccggt
     4441 ggtgtccgtc ggcaatataa agtgatggaa tctccgcaaa aattccggta atttgtgcta
     4501 ttgttttttc atcgtcaatt ttccagaaag tgtgaccgaa accgtcaaat tcggctacga
     4561 aatcgtattc cggagtgttt tttgaaatat cttttacaat attatccagt tcttcgtggt
     4621 gaggataagc aaaaaatacc ggttcgatgt tggcattatt cacccgaacg tgtttcatgc
     4681 ggtcttcttc cttatcgcgg cgggtaagtt cgtgtttttt gatttttccg gtcatgtaat
     4741 cgtccacata agcgcatact accaatccgt actgagtgcg cccgttcatt gtttgagcgt
     4801 aaacgtaata tttttcgttc tcatcctgca caagccagcc gtttgagcgg aatttggcaa
     4861 aattttcggc cgctttcgtg taaactttgt cttcgtgttc gtcagtgcct accggaaaat
     4921 ctatttccgg cttgatgatg tgataaagtg aatacggatt gcctgcagct tctgcccgtg
     4981 cttcgtcaga atttaacaca tcgtaggggc gtgaagccac tttttcaacc agatttttag
     5041 gaggacgaat gcctttgaac ggtttaattg ttgccataaa tagtttgtag tttattgtaa
     5101 aatagtttgt aatagcatta ttatgactta ttttttaatt aactttcgca aatgtaagta
     5161 gttgtaatta aagcatttac aaatatgctt acaattttct acttgcgttg acctgtcccc
     5221 ctctccttgg ggagaggggt taggggtgag cctgcctgcc gcaggcaggg tcgaatgata
     5281 agaatatttg aaagatcatt tttgtataaa catctgatag tcaatatatg c
//
LOCUS       DAORKP010000143         5289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3442_length_5289_cov_8.893198, whole genome shotgun sequence.
ACCESSION   DAORKP010000143 DAORKP010000000
VERSION     DAORKP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_5289_cov_8.893198"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLN75_07815"
     CDS             <1..1167
                     /locus_tag="PLN75_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HRG04062.1"
                     /translation="TCNLDCAYCYYLEKEKLYQNRKKLEMTDEVLEMFTKSYIEAQPV
                     KEVLFTWHGGETLLRDISFYKKALQFQKKYGRGRQIDNVLQTNGTRLNDEWCRFFKDN
                     NFLVGISIDGPEHCHDVYRKNKGGKGTFAEVMKGVGLLQKHGVEFNTLSVINDYNVQF
                     PLDVYRFFKEIGSRYMQFAPIVERISDGRTDGLKLLPPDQNIDYQLASWSVNPVKFGK
                     FYIEIFDEWVRYDVGNFYVQLFDATLANMVGAQPGVCIFAETCGHAAAMEFNGDVYAC
                     DHYVFPEFKLGNVRNQTIFEMMFQQQQLQFGADKKEKLPTQCLQCDVLRFCHGECPKN
                     RIARTSDGEPGLNYLCEGYKAFFRHVLPYMEFMANELHNKRPPANVMYFARGKK"
     gene            complement(1182..2192)
                     /locus_tag="PLN75_07820"
     CDS             complement(1182..2192)
                     /locus_tag="PLN75_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRG04063.1"
                     /translation="MRNKIHYLYRIFKTGFGVQKLLNVAKLELSYRLSVFGILTRWNV
                     SPQYISIEPANVCNLHCPECPVGMRQTAVKPVFADLALIHQVVDELSPALMYATLYFQ
                     GEPLLNKNFTEIVKHLHSKNIFTATSTNAQLIDDRMAKNLVESGLDRLIISVDGTTQE
                     TYEAYRAGGSLEQSIRAIQALTEWKNRLKSPSPQIEIQFLVLKTNEHQLDEMKKLARE
                     WKADKLTFKSAQLYDFENGHELLPENKKYARYELRQDGKYHIKNTLKNRCKRLWKGAV
                     ITSGGDVLPCCFDKDSRFVFGNLRSKTFGECWQSEAAYGFRKKLLQNRKQFDMCRNCT
                     SP"
     gene            2350..4053
                     /locus_tag="PLN75_07825"
     CDS             2350..4053
                     /locus_tag="PLN75_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG04064.1"
                     /translation="MSKKILFLVTIASAVIFTSCSKKLTALDAAYIKTNPNPLEMKAG
                     EVNATVTGTFPQKYFNKKAVVTVTPVLKYGNKETKAQSAVFQGEKVLGNDRMISYKAG
                     GTYEMNPKFMFDPEMAKSELYLNFDVAQKKKTYKLPALKIADGVISTAYLASTSPKEL
                     QPALSPDNFQRITQENQDADILFLIQQAQLRNSELNKDDIKQFSAKVKDARDAQNKEI
                     SSLEVLGYASPDGSLDLNTNLAEKRQKVTTDYINKELKKLQANVSVDSKFTAEDWDGF
                     QKLMEASNIQDKDLILRVLSMYQDPEQREREIKNLASTFQSIAGEILPQLRRSRLKMS
                     VDVTGKSDAEIMSLASTSPKDLTANELLYAGTLATTTKEQADIYENFIDLHPEDARGY
                     NNLGLMLYYDGDVKEAERLFEKSLSLNPASPDANFNLGLVKLGKGLLTDAQAFFGKSA
                     GTAGNLNAALGTSYIAQGDYTKALNVMRGSNTNNAALVQILNNDYNGARNTLAAVKNP
                     NALTAYLGAILAARTNDRDTVYSNLRSAVQQDRTLIKKALNDLEFAKYFADANFLSIL
                     K"
     gene            4381..>5289
                     /gene="holB"
                     /locus_tag="PLN75_07830"
     CDS             4381..>5289
                     /gene="holB"
                     /locus_tag="PLN75_07830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRG04065.1"
                     /translation="MFSDIIAQQSTKDRLIRSVREKRIPHAQLLHGPEGVGKLSLAIA
                     FAQYICCKNKTDKDACGVCPSCKKYSQLAHPDLHFVFPVIKPPGKNTVICDDYLPDFR
                     KMYIENAYFSLEDWYERIGDGAKQGMIYSGESSEIIRKLSLKTYESDYKVMIIWMPEL
                     MHESCANKLLKILEEPPAFTVFLLVSNNPDTIIQTILSRTQHLKVPRLGEDEIIAALT
                     AENPDAEAAEIRNAARVSEGSYLKARKLLTETGEMSLNFDRFKELMRKAWMVGNKKDH
                     DSLKTLKKWADVLAAAEVGRERQKKFL"
BASE COUNT         1572 a         1159 c         1146 g         1412 t
ORIGIN      
        1 acctgcaacc tggattgtgc ctattgttat tacctggaaa aggaaaaact ttatcagaat
       61 cgcaaaaagc tggagatgac cgacgaagtg ctagaaatgt ttaccaaaag ctacatcgag
      121 gcgcagccgg taaaagaagt gcttttcacg tggcacggcg gcgaaacgtt gctgcgcgat
      181 atttctttct ataaaaaagc gttgcaattt cagaaaaaat acggaagggg aaggcaaatt
      241 gacaatgtgc ttcaaaccaa cggtaccagg ctgaacgatg aatggtgccg tttttttaaa
      301 gataataatt ttctggtcgg aatttcaatc gacggccctg agcattgcca cgatgtatat
      361 cgaaaaaaca aaggcggaaa aggcacgttt gcggaggtga tgaaaggagt gggtctgctg
      421 cagaaacacg gggtggaatt taacactctc tcggtgataa atgattacaa tgttcagttt
      481 ccgcttgatg tatatcgctt tttcaaggaa atcgggagcc gctatatgca gtttgcaccc
      541 attgtggagc ggatttcgga tgggcgaacc gatggactga aactgctgcc tcccgaccaa
      601 aatattgatt atcagcttgc ttcgtggagt gtgaatccgg tgaagttcgg gaaattctat
      661 atcgaaatat ttgatgaatg ggtcagatac gatgttggta acttttacgt tcagcttttc
      721 gatgccacgc tcgccaacat ggtaggcgca cagcccggag tttgtatttt tgctgagact
      781 tgtggacatg ctgctgccat ggaattcaac ggcgatgtgt atgcctgcga tcattacgtt
      841 tttccggaat tcaaactcgg gaatgtgcgg aatcaaacca tattcgaaat gatgtttcag
      901 cagcagcagc ttcaatttgg tgctgataag aaagaaaaac tccccactca gtgtcttcag
      961 tgtgatgtgc tccggttttg tcacggcgaa tgtcccaaga accgcattgc ccgaacttcg
     1021 gatggcgaac cgggcctcaa ttatttatgt gaaggttata aagcgttttt cagacatgtg
     1081 ttgccttaca tggaattcat ggcaaacgaa ctgcacaaca aacgtccgcc tgccaatgtt
     1141 atgtactttg caagggggaa aaagtaaatg cccgcaatac gtcagggaga ggtgcagttt
     1201 cggcacatat cgaattgctt ccggttttgg agcagttttt tcctgaaacc gtaagcggcc
     1261 tcactttgcc agcattcacc gaaggtttta ctcctcagat ttccgaatac aaaccgactg
     1321 tccttatcaa aacagcacgg gagcacatcg ccgcccgagg tgattacagc tcctttccat
     1381 aaacgtttgc agcggttctt gagtgtattt ttgatatggt attttccatc ctgacgaagt
     1441 tcgtaacgcg catatttttt attttcgggc agcagttcat gtccgttttc aaaatcataa
     1501 agttgtgctg atttgaaagt cagtttgtcc gctttccatt ccctggccag tttcttcatt
     1561 tcatccagtt ggtgttcgtt ggttttcagc accagaaact ggatttcgat ctgcggcgac
     1621 ggtgatttta atctgttttt ccattcagta agcgcttgta ttgcacggat tgattgctcc
     1681 agactcccac ctgcacgata tgcttcgtag gtttcttgtg tggttccatc caccgaaatt
     1741 atcagccggt cgagtcccga ttctacaaga tttttagcca ttctgtcatc gataagttgt
     1801 gcattggtcg atgtagcggt gaaaatattt ttgctgtgca ggtgttttac tatttcggta
     1861 aaatttttgt tgagcaatgg ttctccctgg aaatacagcg tggcgtacat caaagccggc
     1921 gaaagttcgt ccaccacttg atgaatgagc gccaaatccg caaaaacagg ttttacagct
     1981 gtttggcgca ttcctaccgg acattccgga caatgcaggt tacatacatt tgccggttct
     2041 attgaaatat actgcggaga tacattccat cgggtaagaa tcccgaaaac cgacagccgg
     2101 taggagagtt cgagtttcgc aacgttgagc agtttttgaa caccgaagcc tgttttgaaa
     2161 attctgtaca aatagtgaat tttatttctc atttttcacc aacgccgaat tgtaataatt
     2221 tattatagca aatcaaaaat ataaaaaaca cgatgcaaag taagttaaaa attggtttaa
     2281 aaggcgtata tttgcacaag aataataaaa aaacaaatac tatattaccc aattattttt
     2341 aagatcacta tgagtaagaa aattttattt ttagtcacaa tcgcatcagc tgtcattttt
     2401 acatcatgca gcaaaaaatt aacggctctg gatgccgctt acatcaaaac aaatcccaat
     2461 ccgctcgaaa tgaaagccgg tgaggtgaat gccacagtaa caggtacttt tcctcagaaa
     2521 tatttcaaca aaaaagcggt tgttaccgtc actcctgtgc tcaaatacgg caacaaggaa
     2581 acaaaagctc agtctgccgt ttttcaggga gaaaaagtac taggaaacga cagaatgatt
     2641 agctacaaag ccggtggtac atacgaaatg aatccaaaat tcatgtttga ccccgaaatg
     2701 gcaaagtcag aactttattt gaattttgac gtagctcaaa agaaaaaaac ctataaactt
     2761 ccggctttga aaattgctga tggagttatt tcaacagcat atctggcttc tacctcaccc
     2821 aaagaactgc agcctgcgct ctcgcccgat aattttcaac gcattacgca ggaaaatcag
     2881 gacgccgata ttctcttcct cattcagcag gcacagcttc gcaacagcga actcaacaaa
     2941 gatgatatta aacagttttc tgccaaagtg aaagatgccc gcgatgctca gaacaaggaa
     3001 atttcatcgc tcgaagtgct tggttatgct tctcccgatg gatctttgga tctgaatacc
     3061 aacctggcag aaaaaagaca aaaagttacg accgattaca tcaacaaaga attgaaaaaa
     3121 cttcaggcaa acgtatcggt cgacagtaaa tttactgccg aagactggga cggtttccag
     3181 aaactgatgg aagcatcgaa tatacaggat aaagatttga ttctgcgtgt actttcgatg
     3241 tatcaggatc ccgagcagcg cgaacgcgaa atcaaaaacc ttgcatcgac attccagtcc
     3301 atcgccggtg aaattcttcc tcaattgcgt cgttcacgcc tcaaaatgtc cgtagatgta
     3361 accggaaaat ccgacgccga aataatgagt ttggcatcta cttcgcctaa agaccttaca
     3421 gccaacgagt tgttgtatgc aggaacgctt gcaaccacaa ccaaagaaca agccgatatc
     3481 tacgaaaatt tcatcgatct tcatccggaa gatgcccgcg gatacaacaa cctgggtttg
     3541 atgctttatt atgacggtga cgtgaaggaa gctgaaagat tgttcgagaa atcactttca
     3601 ctcaatccgg cttcacccga tgcaaacttc aatcttggac tggtgaaact gggcaagggc
     3661 ctccttacag atgcacaggc attttttgga aagtcagccg gcacggctgg caatttgaat
     3721 gcagcactcg gaacaagcta catagctcag ggagattaca ccaaagcact gaatgtgatg
     3781 cgcggatcga acaccaacaa tgctgccctg gtacagattc tgaataacga ttacaacggt
     3841 gccagaaaca cgctggctgc tgtgaaaaac ccgaatgccc ttactgcgta tctcggagcc
     3901 atactggcag cacgcaccaa cgaccgcgat acggtgtaca gcaatctgcg cagcgccgta
     3961 cagcaggatc gtacactcat caaaaaagca ctcaacgatc ttgaatttgc aaaatatttc
     4021 gctgatgcga attttctgtc aattttgaaa taattttttc ttatagttta tttttaatcc
     4081 cggtattcat gcatgagtat cgggattttt ttttcgataa acaatactga cttttaataa
     4141 gctgatgact taaaaataaa agaaggtttc gtgcctttta ttatttgttt ctcacagtcg
     4201 aaaaccctga ttccacgctg cctacgaaat atgtaacaca catttttgtt ttcttaaaat
     4261 tttaatcaag tttatgtaat aaaaaattat tctcgtacct ttgtcattat aaagaaattg
     4321 cattactccg cttccaccgg aaaccgatgc aaacagacag cactataaat tgataatcaa
     4381 gtgtttagcg atataatagc tcagcaatcc accaaagacc gtttaatccg atcggtcagg
     4441 gaaaaacgca taccccatgc ccagcttctt cacggaccgg agggtgtggg caagctctcg
     4501 ctcgctattg cctttgcaca atacatttgc tgtaaaaata aaacagataa agatgcctgc
     4561 ggtgtttgcc cttcgtgcaa gaaatacagt cagctggctc atcccgattt gcactttgta
     4621 tttccggtga taaaacctcc cggcaaaaac accgtgatat gcgatgatta cctgcccgat
     4681 ttcagaaaaa tgtatattga aaatgcatat ttcagtctcg aagactggta cgaacgcatc
     4741 ggcgacggag ccaaacaggg aatgatttat tccggcgaaa gctccgagat catccgaaaa
     4801 ctgagcctga agacctatga gtccgattac aaagtcatga tcatctggat gccggaactg
     4861 atgcacgaat cctgtgccaa caaattgctg aaaattctgg aagaaccgcc tgcgtttacc
     4921 gtttttctgc tcgtatccaa caatcccgat acgattattc agactatact atccagaaca
     4981 cagcacctga aagttccccg cctgggtgag gacgaaatta ttgcggcact tacggctgaa
     5041 aatcccgatg ccgaagctgc tgaaattcgt aatgcagccc gggtgtcgga gggcagttac
     5101 ctgaaagccc gaaaattact gaccgaaaca ggtgaaatgt cacttaattt tgacaggttc
     5161 aaagagctga tgcgaaaagc ctggatggtg ggcaacaaaa aagaccatga ctcactcaaa
     5221 acgctgaaaa aatgggccga tgtgctggcc gctgcagagg tggggcgcga aaggcagaaa
     5281 aaattcctt
//
LOCUS       DAORKP010000144         5284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3448_length_5284_cov_7.332377, whole genome shotgun sequence.
ACCESSION   DAORKP010000144 DAORKP010000000
VERSION     DAORKP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_5284_cov_7.332377"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PLN75_07835"
     CDS             complement(<1..1388)
                     /locus_tag="PLN75_07835"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRG04066.1"
                     /translation="MQVQFRLNYQALWGQSIYLQVYTGDEQYVRHEMICKGVNLWLVK
                     INLDENATEIQYRYAIRNQDKTFENEFGLMRRLKIPKTDKIIALEDYWRIPNGDSAFN
                     TLAFTDCFFNRKNAPKSKPSVNINFILRINCPQMEPDRHFAIIGNQEALGNWDVSKKV
                     RLDETLYPFWSIALNTHDIKFPLEYKYLIVDTKSDEVLAWGGGPNRTVLNADEEKLTI
                     VTDENFIRTIPSWKTAGVAIPVFSLRSEEGFGIGEFLDLKKMVDWAKLTGQRIIQTLP
                     INDTILNHTNYDSYPYNAVSVYALHPIYLRLDRLGVPKYKKDIVYFENKKKELNENSF
                     SDYQNVLNEKWKYFQLIYPKESPAVFASDDYRQFFESNKTWLVPYAVFSYLRDKNETP
                     EFGKWGEFSTYNADEIVAFADEGNPQYPEIALFYFLQYHLHKQLIEVHEYARSKGVAL
                     KSDIPIGVSPRSV"
     gene            complement(1531..1601)
                     /locus_tag="PLN75_07840"
     tRNA            complement(1531..1601)
                     /locus_tag="PLN75_07840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1567..1569),aa:Cys,seq:gca)
     gene            complement(1756..2232)
                     /locus_tag="PLN75_07845"
     CDS             complement(1756..2232)
                     /locus_tag="PLN75_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04067.1"
                     /translation="MRNLKYIVLSTLMVLTLTAQAQRERHGHPNVKEMHERKWQFVMN
                     EVSLSPAEMNAVKPVFLDYEKKMWELHMKNREYFRMGVEKKQDAAINYAELNDKAVNS
                     EISQAQLLRAYHMKLRKLLKPETLYYYYQAERKFKRKLLQNMPKNMPPPPDEELKR"
     gene            complement(2247..2573)
                     /locus_tag="PLN75_07850"
     CDS             complement(2247..2573)
                     /locus_tag="PLN75_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04068.1"
                     /translation="MMEKKNISLDKIDKKLPFSVPENYFEDFAARMELQVAGETLQPP
                     HRHILNWRVAVAAVFAGILLAGAIYTTIFKNASKSDNYESYVLSQVDESSMLYYYVGE
                     PTTETK"
     gene            complement(2626..3174)
                     /locus_tag="PLN75_07855"
     CDS             complement(2626..3174)
                     /locus_tag="PLN75_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRG04069.1"
                     /translation="MNSSNISFNSLSHKDKATAFSGIVRTYQERLYWHIRKMVLSHDD
                     ANDILQDVFMKAWNGLDNFRGDSQVSTWLYRIATNETLTFLAAKRMKNINSLDEVEDV
                     LLQNLKADSYFNGDETQLQLQKAILALPEKQRLVFNMRFYDDLKYEEMSEVLGTSVGA
                     LKASYHHATKKIEQILTGKIED"
     gene            complement(3250..4263)
                     /locus_tag="PLN75_07860"
     CDS             complement(3250..4263)
                     /locus_tag="PLN75_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRG04070.1"
                     /translation="MSKKMKVFRDYFFFIGIAILFFMAYKLGWKTIVDNLGQTGWWFL
                     AIVGVWIFVYLANALSLAIIIRDGSEESKRVSLMHLLKLTISAYSINYITPLAIGGEP
                     YKALELKPDLGTHKATSSVLLYVMMHYVSHFFFWMISVPLFLLIIPKIPPGVQIILWG
                     MIFGSILLIYWAFTVYTKGIIRKALAIGCRIPFFGKKIRIYRDKNKERFDEMDELIAN
                     LFTNRKKDFFLSLGVELLSRYISCLEIYFMVIPMNYDITFIQCILIYSFATLVANIFF
                     FAPMQMGTREGGFALAVSLLYVPAGLGVYIGLCTRIREFFWIVVGIGLMKLKIPDRLQ
                     IRS"
     gene            complement(4279..5157)
                     /locus_tag="PLN75_07865"
     CDS             complement(4279..5157)
                     /locus_tag="PLN75_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRG04071.1"
                     /translation="MKTEIVASLKSIETENYLDRVFYRPIGFKIAKALIGTGITPNMI
                     TILSLFVGSVAGVLWYFPYSLTFAVVGILSLIVANIMDCVDGQLARLTGIKSEIGRIL
                     DGFAGDFWFLLIYIAFAHRLNVQFDTWLFWLAALLMAISHLNQAAITDYYKTLHLFFI
                     SKEKGKEFESSERIRARIATMPAGVNRFFSRFYLIYTLSQEKQTPEVQKYLAALKEKY
                     GEDIPEDVRLAFRTKSRQLLPMIDMMTFNGLSGILFLSVLLNAVWIYLVWVIVILNIV
                     NKIIRLRHEKMCKSLG"
BASE COUNT         1515 a         1096 c         1077 g         1596 t
ORIGIN      
        1 acactgcggg gacttactcc tattggaata tcacttttga gtgcaactcc tttgctgcgg
       61 gcgtattcat gaacttctat cagttgtttg tgcaaatgat attgcagaaa ataaaacagg
      121 gcaatttccg gatattgtgg attaccttca tcagcaaaag ccactatctc atcggcattg
      181 taggtactga attcacccca tttcccaaat tcgggtgttt cattcttatc ccggaggtac
      241 gaaaaaaccg catacggcac cagccaggtt ttattggatt cgaaaaattg acggtaatca
      301 tccgaagcaa atacagccgg actttcttta ggatatatta attgaaaata cttccatttt
      361 tcgttcaaaa cgttctgata atccgaaaaa ctgttctcgt tcagttcttt ctttttgttt
      421 tcaaaataaa caatatcctt tttgtacttc ggcacaccca atcggtccag ccgcaggtaa
      481 atgggatgca acgcatagac cgaaacggca ttatagggat acgaatcgta attggtgtga
      541 ttcagtatgg tgtcgttgat ggggagtgtt tgaattatac gctgtccggt gagtttagcc
      601 cagtccacca tttttttcag atcgagaaac tcaccgattc cgaaaccttc ttcacttcgg
      661 agtgagaata cgggaatggc cactcctgcg gttttccacg agggtatcgt ccggatgaaa
      721 ttttcatcgg tgacgatggt taatttttcc tcatcagcat tgagtactgt tcggttgggt
      781 ccgccacccc aggcgagcac ttcatcggat ttggtatcca ctatcaggta tttgtattcc
      841 agtggaaatt ttatatcatg ggtattgaga gctattgacc agaaaggata taaagtttca
      901 tccaacctca cttttttgct tacatcccag tttcccaggg cttcctgatt tccaataatg
      961 gcaaaatgcc ggtcgggctc catttggggg caatttatcc gcaggatgaa attgatatta
     1021 acggatggtt tacttttggg agcatttttt cgattaaaaa agcagtcggt aaatgccagt
     1081 gtattgaagg cactgtcgcc atttgggatg cgccagtaat cttccaaagc aattatctta
     1141 tccgttttgg ggatttttaa tctccgcatg aggccaaact cattttcaaa cgttttatcc
     1201 tgatttcgga tagcgtagcg gtattgtatc tctgtggcgt tttcgtccag atttattttg
     1261 accaaccata aatttacgcc tttacatatc atctcgtgtc gaacgtattg ttcgtcgccg
     1321 gtatagacct gtaagtaaat ggattgtccc cagagagcct gataattgag tctgaattgt
     1381 acctgcatag gtgtaaagta ttattgtgtg caaatttaca aattaataaa cctgctcaaa
     1441 agaaataacg aattgcctaa cgaaatgaga gctaaaacgt ttgataaaaa acaccctcta
     1501 taaattttat tttatagagg gcgtctgatg aggtacttgg cggattcgaa ccgccgtaaa
     1561 cggttttgca gaccggtgcc tagccactcg gccaaagtac cctggttttg gactgcaaag
     1621 atagtattat tttgtcagtt gacaaaattt catttcggtt ttttctatag cccgttgata
     1681 acaaccatta aaaaaaagaa cacaacacaa aaaatcaatt gtgttgtgtt ttttgcacat
     1741 tcatatagga tgattttacc ttttcaattc ttcatcagga ggtggaggca tgtttttcgg
     1801 catattctgc agcagttttc gtttgaattt tctctctgcc tgatagtaat aataaagagt
     1861 ctcgggtttg agtaatttgc gcagcttcat gtgatatgcc ctgagtagct gcgcctgcga
     1921 tatttctgaa tttaccgctt tatcattaag ttcagcatag ttgatagcag catcttgttt
     1981 tttctctacc cccatccgaa agtactccct gtttttcatg tgaagttccc acattttttt
     2041 ttcataatcc agaaataccg gttttacggc attcatttcg gcaggcgaaa gcgaaacttc
     2101 attcatcaca aactgccatt ttctttcgtg catctctttc acattcgggt gtccatgtcg
     2161 ttcccgctgt gcctgagctg tcaacgtgag caccatcaat gtgcttaaaa cgatatactt
     2221 tagatttctc attggtcagg atttttttat ttggtttctg tagttggttc acctacataa
     2281 taatacagca tggaggactc gtccacctgc gacaatacat aggattcata attatcagat
     2341 tttgaagcgt ttttaaatat tgtggtatat atagcgcctg ccagaagtat tcccgcaaaa
     2401 acagcagcca ctgcaaccct ccagttcaat atgtgacggt gaggtggttg cagtgtttca
     2461 ccagcgacct gcaattccat gcgagctgca aaatcctcaa aatagttttc gggaaccgag
     2521 aatggtaact tcttatctat tttatccagc gatatgtttt tcttttccat catgttgttt
     2581 tgactgtttt tagcataaaa ggtttaaata tctcattctt aaaacttagt cttcaatttt
     2641 tccggttaga atttgctcaa ttttcttagt ggcatgatga taggatgctt tcagtgcacc
     2701 tacggaggtt ccgagcacct cgctcatttc ttcatatttc aagtcgtcgt aaaaccgcat
     2761 attaaatacc aatcgttgtt tttcgggcag ggcgaggatg gctttttgca gctgcaactg
     2821 ggtttcgtcg ccgttaaagt aagaatcggc ctttagattt tgcagtaaca catcttccac
     2881 ttcatccagc gaattgatat ttttcattct tttggcggcc aaaaaagtga gggtttcgtt
     2941 ggtggctatc cggtaaagcc aggtagatac ctgcgaatca ccccgaaaat tatcgagtcc
     3001 gttccaggct ttcatgaata catcctgcag tatatcattg gcatcatcgt gcgaaagcac
     3061 cattttccgt atgtgccagt acaatcgctc ctgataggtg cgtacaatgc ccgaaaatgc
     3121 agtcgctttg tctttgtggg acaatgagtt gaatgaaata ttgcttgaat tcaattgttt
     3181 gcttttagag gttagacttg caaagcccca aaaggtttaa tcgggatcag tataaaagaa
     3241 ctgtaaagtt tagcttcgga tttgaagtct atcggggatt ttcagcttca tgagtccaat
     3301 tcccaccaca atccagaaaa actcgcggat acgggtgcat agtccaatgt aaactcccaa
     3361 cccggcaggc acgtacaaca ggctgactgc caatgcaaaa ccaccttcac gcgtccccat
     3421 ctgcatgggc gcaaagaaga atatattagc aacaagagta gcgaatgaat agataagaat
     3481 acactggata aacgtaatat cgtaattcat gggaatgacc ataaaatata tttcaagaca
     3541 tgaaatgtaa cgggaaagca actccacgcc cagcgacaag aagaaatcct tcttccggtt
     3601 tgtaaaaaga ttggcgatta gttcatccat ttcatcaaac cgttccttgt tcttatccct
     3661 gtagattctg atttttttcc cgaaaaacgg aatccggcaa cctattgcca gtgctttacg
     3721 tataatccct ttggtataaa cggtaaatgc ccagtaaatg aggaggatgc ttccaaagat
     3781 cattccccag agaattattt gcactccggg tggaattttg ggaataataa gcaaaaagag
     3841 tggaacagaa atcatccaga agaaaaaatg cgagacataa tgcatcatca cataaagcag
     3901 aacagaagaa gtggctttgt gggttcccag atcgggttta agttcgagcg ctttataagg
     3961 ttcaccgcct atggcaagtg gtgtgatata attgatacta tatgccgaga tcgtcagctt
     4021 gagtaaatgc atgagtgata cgcgttttga ctcttcactg ccatcccgta tgatgattgc
     4081 aagtgacaaa gcattggcca ggtatacaaa tatccaaaca cccacgatgg ccagaaacca
     4141 ccatcccgtc tgtccgagat tgtccacaat ggttttccat cccagtttgt aagccatgaa
     4201 aaacaaaatc gcgattccga tgaaaaaaaa gtaatcgcga aacactttca tttttttcga
     4261 catgtcgaaa cttttatttt aaccaagcga tttacacatt ttctcgtgtc tgagcctgat
     4321 tattttattg actatattca aaatgacaat aacccacacc agataaatcc atactgcatt
     4381 gagaagcaca gaaagaaaca gaatgccgga caggccattg aaagtcatca tatctatcat
     4441 gggcaacagt tggcggcttt tagttctgaa cgccagacga acatcctcgg gtatatcttc
     4501 accgtacttc tctttgagag cggccaggta tttttgtact tcgggagttt gtttttcctg
     4561 actaagggta tagatgaggt aaaatcggga gaagaaacgg tttacaccag ctggcatagt
     4621 ggcaatgcgg gctctgatgc gctccgacga ttcaaattct ttcccttttt ccttactgat
     4681 aaaaaagaga tgcagggttt tgtaataatc ggttatggca gcctgattga ggtgtgaaat
     4741 agccatcagt agagctgcga gccagaatag ccaggtgtcg aactgtacat taagccggtg
     4801 tgcaaaagcg atataaatga gaagaaacca aaaatcaccg gcaaatccgt ccagtatacg
     4861 accaatttca gacttaattc ccgtcaggcg ggctaactgg ccatccacac aatccattat
     4921 attagctaca atcaacgaca ggatgccaac gacagcaaat gtgagcgaat agggaaaata
     4981 ccataaaaca ccggctaccg atcccacaaa aagtgaaaga atagtgatca tattgggtgt
     5041 aatacccgtg ccaatcaatg cttttgctat tttaaatcca atggggcggt aaaatactcg
     5101 gtcgagataa ttttctgttt ctatggattt aagggaagct acgatttctg tcttcataag
     5161 ttacgtctaa tgttatactt caaatactta aaaataaact gacgaacatt ttttcaaata
     5221 tttttggtca aacatctgtc agcgacaacg aaaaataatc ataatatttt gtgaacgtgc
     5281 agac
//
LOCUS       DAORKP010000145         5259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3480_length_5259_cov_7.841852, whole genome shotgun sequence.
ACCESSION   DAORKP010000145 DAORKP010000000
VERSION     DAORKP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3480_length_5259_cov_7.841852"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..100)
                     /locus_tag="PLN75_07870"
     tRNA            complement(26..100)
                     /locus_tag="PLN75_07870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Val,seq:tac)
     gene            complement(404..2035)
                     /locus_tag="PLN75_07875"
     CDS             complement(404..2035)
                     /locus_tag="PLN75_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HRG04072.1"
                     /translation="MIKNLLTASLLLLASGALFAGGLLTNTNQSVHFLRNPARDASIE
                     IDAAYSNPAGVVFLSDGFHFSLNNQSAFQTRTITSTFAPFAGNGGSATKEYSAKTSAP
                     VIPSFQAAYVKDRFAISGSFGVIGGGGKAVFKEGLPSFEAPVSMIPLSLTSNKILTSS
                     YSLDQYMEGQQYTFGAQLNGAYKITERLSASVGLRVNIVSNGYKGHLTSIMINPNQPA
                     FGAAYNGNAMVSASKFFTDAATTLTGWSAGANSYAAGLQPIVSGGGGNYPLSSGTSVG
                     LTSVQVAQIQALLGATGLTPAQIGGMSILDAKNALTAAGPAFAQKATAMTANAAGTAD
                     KQLETKQAGVGFAPIFSLNYNLDRLNLAVKYEMKSTITLTNETTVDETGMYADGAETP
                     YDIPAYLAAGADYHITDSWKISAGYHHFFDSDAKMANNKQQYINGGGNEYLFGTEYKF
                     AKRFLLSAGGQLSRTAVTDEYQTDMSFSLNSMSVGLGGAVDLTKNLRLNVAYMFTNFS
                     DWTKSSANYNGTTLAGTDVYARTSKAFGIGIDYRF"
     gene            complement(2489..3067)
                     /locus_tag="PLN75_07880"
     CDS             complement(2489..3067)
                     /locus_tag="PLN75_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRG04073.1"
                     /translation="MSFSLVQITSTFIALFIIIDILGAIPILIGLKEKGIQIKSLSVS
                     VVSFVILLIFLFAGDWILQLFNVDIQSFAVAGSLVIFIFAIEMVLDVEIYKNRGPEGS
                     ASFVPIAFPLVAGPASFTTLLSLRAAYDVQNIIVALVINIIWVYLVLKFTGKIEKFFG
                     KGGIYIMRKFFGIILLAISVKLFTENIGSLLK"
     gene            complement(3098..3904)
                     /locus_tag="PLN75_07885"
     CDS             complement(3098..3904)
                     /locus_tag="PLN75_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRG04074.1"
                     /translation="MLNASIVLFNHSIDDIKPLVESLRSSALMNDVFLIDNSPAEVVG
                     FRNLPAVYIFNNKNLGYGAAHNIALHKTLEQNVLYHLVINPDIQLDYRILDELTDYMD
                     KNPDIGHIMPRVLNPDGTIQYLCKLLPRPSDLLFRRFLPTGWTQKSNEHFELRKSGYD
                     KIMEVPYLSGCFMLLRTEALKNVGLFDERFFMYPEDIDLTRRIHRKYKTIFYPNVSVV
                     HHHARASYQNVRLLAVHVWNLVRYFNKWGWFFDKERKKVNEFVLRKLDLL"
     gene            complement(3906..5018)
                     /locus_tag="PLN75_07890"
     CDS             complement(3906..5018)
                     /locus_tag="PLN75_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRG04075.1"
                     /translation="MNYKIAFIGNSSLTMHNFRINLLRSMVKQGYEVVVIAPKDSDTT
                     SYKSNKIRYIPVDIDCKGLNPIRDILLMNRLRALYQKEKFDFIFHYTIKPVIYGSFAA
                     RLTGIKHISVITGLGYTFLNTGFLNKLVISLYRHSLKKAEEVWFLNEDDREVFLTNEV
                     VNKKRTKVINGEGVNARLYQPAKKESSRIKFLFVGRMLWDKGIGEYVEASRILKKKYP
                     NVVFEALGSLGSANPSAISREQMDEWEKEEIIKYLGETHRVIPFLSNSSCVVLPSYRE
                     GISRVLLEASAMEKPVVATNVTGCRDIVVDGYSGYLCEVRNPESLAEAMEKIIRLPKE
                     KLEEMGKEGRRLVLEKFDEKIIIQEYVSKLKEIFPE"
     gene            complement(5050..>5259)
                     /locus_tag="PLN75_07895"
     CDS             complement(5050..>5259)
                     /locus_tag="PLN75_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRG04076.1"
                     /translation="LPFPDIHIEHDTWIGICAYKVGSVHIADEVLMNYYRHGQNASPI
                     AENRTNSLSIKLLRRWYMLIAYLKY"
BASE COUNT         1575 a         1154 c         1013 g         1517 t
ORIGIN      
        1 tttctacatt cgacttttat gaaagtgggc gttgacggat tcgaaccgcc gaccctctgc
       61 ttgtaaggca gatgctctga accagctgag ctaaacgccc gtattaattt caaaaaactt
      121 ctttttactg atgccgaccc ctccgattta tcggagtgct ctgaaccagc tgagctaaac
      181 gcccttgttt tttctaaagt gatgcaaagg tactacaatt tttaaaacta ccaaaaaact
      241 cttcaaatat ttctgaaata ttttttataa gaccccaaaa acggaaattc gttccacaat
      301 cttcttagca ctataaaaaa aacggaacca attctttcaa tcagttccgt ttaagagtag
      361 aagataaaac gatttattcc cggggaaaat cgctttgcta ttttcagaat ctgtaatcta
      421 tcccgatacc aaatgctttg ctggtgcggg cataaacatc tgttccggcc agggtggttc
      481 cgttgtaatt tgccgaagat tttgtccagt ctgagaagtt ggtaaacatg taagccacgt
      541 tcagacgaag atttttagtc agatcaactg caccacccag tcctaccgac atggagttga
      601 ggctgaagct catatctgtc tgatactcat cggtcaccgc cgtgcggctc aactggcctc
      661 ctgcgctcag caggaacctt ttggcaaact tatattctgt accgaacagg tattcatttc
      721 cgcctccgtt gatgtactgc tgtttgttgt tggccatttt ggcatccgaa tcaaagaaat
      781 ggtgataacc ggcagaaatt ttccagctgt cagtaatatg ataatcggct ccggctgcca
      841 ggtaagcggg tatatcgtag ggagtttctg ctccgtcggc atacatgcct gtttcatcta
      901 cggtggtttc gttggtcagt gtgattgtac ttttcatttc gtattttaca gccaggttca
      961 gccggtccag gttgtaattc agactgaaaa tcggtgcaaa tcccacacct gcctgtttgg
     1021 tttcaagctg cttatcggca gtaccggcag catttgcggt cattgcagta gctttttggg
     1081 caaaagctgg accagccgct gttaatgcgt ttttagcatc caaaattgac atgccgccta
     1141 tctgagcagg agtaagtcct gtagctccga gcaaagcctg aatctgggca acctgaacag
     1201 aggtcagtcc aactgaagtg ccgcttgaaa gcggataatt tccaccgcca cctgagacaa
     1261 taggttgtaa accggcagcg taagaattgg cacctgccga ccaaccggtg agtgtagtag
     1321 ccgcatcggt gaagaatttg gaggcagata ccatcgcatt gccattgtaa gcagctccaa
     1381 aagcaggttg attcggatta atcatgatac tggtcaggtg tcctttgtaa ccattgctca
     1441 caatatttac acgcaaacca acacttgccg acaaccgttc agtaatttta tacgcaccat
     1501 tcaactgggc accaaaggtg tattgctgtc cttccatata ctgatcgagt gaatacgatg
     1561 aagtcagaat tttatttgac gtcagcgaca gaggaatcat cgaaaccgga gcctcgaacg
     1621 agggcaaacc ttctttgaac acggccttac cgccaccgcc tatcactccg aaacttccgg
     1681 aaatggcaaa acggtccttg acataagctg cctgaaaact tggaattaca ggagcagaag
     1741 ttttagcgct gtactctttg gtggcactgc cgccgttacc agcaaaaggg gcaaacgtag
     1801 atgtgatggt ccgggtctgg aatgcactct gattgttgag cgagaagtga aaaccatccg
     1861 agagaaatac cacacctgcc ggattggaat aagcagcatc aatttcgatg gatgcatcac
     1921 gagccggatt tcttaaaaaa tgaacactct ggttggtgtt agtgagcagc cctccggcaa
     1981 aaagagcacc cgaagccagt aataaaagtg aagctgtaag taagttctta atcataaaat
     2041 tatgttttgt agtttaatga ataacgttca tccgaatttt ctaataattt agggatgaaa
     2101 taaattgtca atttgcaaaa ttacacattt caaagtaaat tgtgcaaatc ataaaataat
     2161 aaatgcacat ttaattaata attgtgcaga tttcattctc acaaactacc aaatcattag
     2221 cgtataacgc tgaatatcaa tgcataagca ccattattct gcatgtgtat atatttgtta
     2281 attttagttt cgatgtgttt aaagaaataa cattacaacc aaatttatag cccaaaggta
     2341 ttctgattca ttttcatttt attgattgaa atcgggctgc acggacttga caggatttga
     2401 aaaacgtagt tgcggggaaa aagaaaagtg aacaaaatct cctgaaaaaa gaattgttca
     2461 cttgtataaa gtgctgatta tatgttgttt atttcaaaag acttccaata ttttctgtaa
     2521 agagcttcac ggaaatggca agtaatatga tgccgaaaaa tttccgcatg atgtaaattc
     2581 cccctttacc gaagaatttc tcaatctttc ccgtaaattt caaaacaaga tatacccaaa
     2641 tgatatttat taccaaagct acaatgatat tctgcacatc gtaggctgcg cgcaatgaca
     2701 acagggtagt gaatgatgcg ggtccggcca ccagcggaaa agcaatggga acaaacgaag
     2761 cactcccctc gggaccgcgg tttttataga tttcgacatc cagcaccatc tcaatggcaa
     2821 aaataaaaat aaccaacgaa cctgccactg caaacgactg aatatccaca ttgaaaagtt
     2881 gcagaatcca atcaccggca aacagaaaaa tcaacagtat cacaaatgat actacgctta
     2941 cactcagcga tttgatttga ataccttttt ctttaagccc tatcaaaata ggtatcgcac
     3001 ccaaaatatc gataatgata aaaagggcta tgaaggtact ggttatttga actaaactaa
     3061 aactcatgac gattgatttt ataaatattc tgcaaaatta caacaaatcc agctttctca
     3121 gcacaaattc atttactttt ttccgctctt tatcaaaaaa ccatccccat ttattgaaat
     3181 acctgaccaa attccataca tgaacagcca aaagtctgac attttgatag gatgcccggg
     3241 catgatgatg aacaaccgag acattagggt aaaaaatagt tttgtatttc cggtgaattc
     3301 gccgcgtcag atcgatatcc tcgggataca taaagaatcg ttcgtcaaac aatccgacat
     3361 ttttcagcgc ctccgtgcgc aggagcatga agcagcccga aagataggga acttccatga
     3421 ttttatcgta acctgatttt cttagttcaa aatgttcgtt tgatttttgg gtccatcccg
     3481 tcgggagaaa acgtctgaac aacagatcgg aaggtctggg gagaagttta catagatact
     3541 gaatggtacc atccggattg agcacccggg gcattatgtg accgatatcc ggatttttgt
     3601 ccatataatc agtcagttcg tccagaatcc ggtaatcgag ctgaatgtcc ggattgatta
     3661 ccaggtgata aagaacattc tgttcgagag tcttatgcag ggcaatgttg tgagccgctc
     3721 cgtagcccag gtttttgttg ttgaaaatat aaacggccgg tagattccga aatccaacga
     3781 cttctgcagg tgaattgtca atcaggaaga catcgttcat cagcgccgac gaacgtagtg
     3841 actcaaccag gggcttgata tcgtcgatag aatgattgaa aaggacaatg gaggcgttaa
     3901 gcatattatt cgggaaaaat ttcttttaac ttgctcacat attcctgaat aattattttt
     3961 tcatcaaatt tctccagaac taaccttcgt ccttcctttc ccatttcttc caatttttcc
     4021 tttggaagcc taatgatctt ttccattgcc tcagcaaggc tttcaggatt tctgacttca
     4081 cacaaataac cagaataacc gtcgacaact atatccctac atcccgttac attggttgcc
     4141 acaaccggtt tttccatagc gcttgcttcg agcagaaccc tggatatacc ttctctgtat
     4201 gatggcagaa ctacacaaga agaatttgaa agaaagggta tcacccgatg agtttcaccc
     4261 agatacttaa tgatctcttc cttttcccat tcgtccattt gttctcttga aatagccgaa
     4321 ggattggcag aacctaaaga gcccaaagcc tcaaacacca cattcggata tttttttttc
     4381 aaaatgcgtg aagcttccac atattcacct attcccttat cccacaacat tctgcctaca
     4441 aaaagaaatt ttatcctgga cgattccttt ttggcaggct ggtacaagcg ggcattcaca
     4501 ccttctccat ttattacttt cgttcgcttt ttattgacca cttcatttgt cagaaatact
     4561 tcccggtcgt cctcattcag aaaccacact tcttcggctt ttttcaatga atgccgatac
     4621 aatgaaatga ccagtttatt caaaaaaccc gtatttaaaa acgtatatcc taagcctgta
     4681 ataaccgaaa tatgttttat ccctgttaat cttgcagcaa atgaaccgta aatgaccggc
     4741 ttgatggtat aatggaaaat gaaatcaaat ttctcctttt gataaagcgc tctcagacgg
     4801 ttcatcaaca ggatatctct tatcggattc aatcctttac aatcgatatc tacaggaata
     4861 tatctgattt tattggactt atagctggtc gtatcgctat ctttgggcgc tataacaacc
     4921 acctcatacc cttgcttcac catgcttctc agtaagttaa tcctgaaatt gtgcatggtg
     4981 agcgagctgt ttcctataaa agctattttg taattcatga tatgtcctga aaactataat
     5041 aattcatatt taatacttca aataagcaat aagcatatac catcgcctca gcaatttaat
     5101 agacaacgaa ttagttctgt tttctgcaat aggtgatgcg ttctgcccat gcctgtagta
     5161 gttcataagt acctcgtcag ctatgtgcac tgacccaact ttgtatgcac aaataccaat
     5221 ccaggtatca tgttcaatgt gaatgtccgg gaaaggcaa
//
LOCUS       DAORKP010000146         5258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3483_length_5258_cov_10.328272, whole genome shotgun sequence.
ACCESSION   DAORKP010000146 DAORKP010000000
VERSION     DAORKP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3483_length_5258_cov_10.328272"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..4106
                     /locus_tag="PLN75_07900"
     CDS             276..4106
                     /locus_tag="PLN75_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRG04077.1"
                     /translation="MKSKLSRVGLLIICFFSFLVFNSFREKTAKPIYLNTSYSFRERA
                     VDLVSRMTTDEKISQLGNTMPPIPRLGVNHYDVWGEALHGVMGRNNNSGMTATSFPNS
                     VAVGATWDPELIKREASVISDEARGFNHDLIFTLTYWSPVIEPARDPRWGRTAETFGE
                     DPFLVSEIGKGFIRGMMGNDPVYLKTVPCGKHYLANNSEFNRHTGSSNMDDRDMREFY
                     LAPYRKLIREYKLPSIMTAYNAVNGVPVSASRFLVDTIARKTYGLDGYVTGDCGAIDD
                     IVRGHFYTKSFEEAAAMGLKSGVDSDCGGVYQNHIQGALKNGLLTIGDIDRALINLFT
                     IRMRLGEFDPKAKVPFAGIKPGIINDPSHHNLAIEIAEKTPVLLKNNKLAQSGENALP
                     INLTKINKIAVLGPQADKVELGDYSGPVEPELRITPLQGIRSYIANNNLSTEVITRSG
                     GNTDRRTDFFSMTGFSTISDGKVKMDFDATKFDEAANGVIASYRFGQNVLQGIKDGDW
                     TLYKNVDISNVDSIRLNTGVSGDGGILEIRVGSASGNILASQEIKPLQNRGNLRNSGR
                     NQIIPVKINNLGISGPQSLVLVYKEAPGSGIDQETLDMAASADVALIFVGTDQSTGRE
                     ESDRFSLKLPGNQNELIQAVSAVNPNTIIVMQTMGMVEVEQFRNLPNVSGIIWTGYNG
                     QAQGEAMAKILFGDVNPGGKLNVTWHKSLSDLPDLNNYTLRGDGSNGRTYWYYNKDVS
                     YEFGFGMSYTTFEYSNFRINKTEITPNDKVTVSVDITNSGKMDGDEVVQIYVKTPDSP
                     SSLERPVKRLKGFKRVTIPRGQSKTVSIEIDCADLWFWDAENDKITFDQGKYIFEIGA
                     SSKDIRGSVQANLSGTYKPVLTTVVAESDLSVLKPGKTIETSLSAALSDDSFLDISKA
                     KVSYRCNNPSVAAVDANGKVTAVGVGVATIFADVTVDGTTVSGSYPIKVMPDLTPKSI
                     SVNGKPVPDFSKDTKAYSYLLDKKSKIPEVKAAAMFPGIRVDIEQAESVPGTAIVRMI
                     DDVTSEINTYYLNFDVKSAGDEFNGNTLGNQWQWIREDPLTNSLSEKSGSLTITSQPG
                     DVSERTNNAKNILLQSTNNDWTIETKLTASRVPSQPENAGILVYQDDDNFVKLMFRAV
                     VKTSQMGRPGAPVQAGTIDLMIEENGISKSFATFNVPNPVTDGNPLILRLEKKGSLYT
                     AYYSLDGNKFDMLGSAVALLKNIRSGLIACDGIITQYMKSIFWFDSDTTKPDSPFNVS
                     FDYFRITNNGLKTK"
     gene            4118..>5258
                     /locus_tag="PLN75_07905"
     CDS             4118..>5258
                     /locus_tag="PLN75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRG04078.1"
                     /translation="MKKLNSFLFLTFFAGTFFGFMQINAQVVEDFKTSKTTQIGKNFP
                     QVNSEGRVRVQISAPDAKLVQLDIGAVKYDLKKDENGVWTGESAPQDEGFHYYQLNID
                     GASVPDPGSLYYYGASRWGSGIEVPAHDQDFYALKNVPHGQVREHIYYSEVNKSMRRC
                     FVYTPPGYDKNTKKRYPVLYLQHGGGENETGWSSQGHANLIMDNLIAEGKAKPFIIVM
                     DNGTWTMSGPRRTEGGTWPPTGWADGFMNTLLKDIVPMIDASYRTIANQKNRAMAGLS
                     MGGMQTRVITLANPDVFSQVGMFSGGSISPDDVNKAPGFREKVKLLFVSYGSYELDNP
                     RTNMNGGPKENTDKLKEMGMNTHFYVSPKTAHEWQSWRRSLYQFAQ"
BASE COUNT         1554 a         1117 c         1217 g         1370 t
ORIGIN      
        1 tggtgtttta gtgtctttgt ggcaaaaaca aggttttcgg agcagactca ttctttcaaa
       61 aaaagaattt tccccggatt cattatgagt tgataatgaa tccgggaata ttgctttgaa
      121 cattgaaata ttaggactat tgatagggag tttagcaaat caatccttat ttttttttca
      181 tcataagttg tatacctcct cattcactgt aggttacatt atatttacct aatcaaaaaa
      241 tcagattgtt ctcagtactt taatacgtaa atacaatgaa aagtaaatta tcccgagtgg
      301 gtttattgat tatttgtttt ttttcatttt tggtctttaa ttctttccgg gaaaaaactg
      361 caaaaccgat ttacctcaac acatcctatt cattcaggga acgtgccgta gaccttgttt
      421 cacgcatgac gacggatgaa aaaatcagcc agttgggaaa tacgatgcca cccattcccc
      481 gcctgggagt aaaccattac gatgtttggg gagaagcctt acacggagtg atgggtcgaa
      541 acaacaacag cggaatgacc gctacttcat ttccaaacag cgtggcagta ggcgctacct
      601 gggatccaga gctgattaaa cgtgaagcct cagttatttc tgatgaggca cgaggtttca
      661 atcacgattt gatatttacg cttacttatt ggtcaccggt gattgagcct gcgcgcgacc
      721 cgagatgggg aagaacagcc gaaacgttcg gtgaagaccc gtttcttgtt tccgaaatag
      781 gcaaaggatt tatccgggga atgatgggca atgatcctgt ttacctgaaa actgttccgt
      841 gcggaaagca ttatctggca aataattccg aattcaaccg ccacaccggc agttcgaaca
      901 tggacgacag ggatatgcgg gaattctatc tggcacctta ccgaaaactc atccgggaat
      961 acaaacttcc gtccattatg actgcctaca atgctgtaaa cggtgttcct gtttcggcca
     1021 gccggttttt ggtggatact attgcccgaa aaacatacgg tttggatggt tacgtgaccg
     1081 gcgactgtgg tgccatcgac gacattgtac gagggcattt ttatacgaaa agttttgaag
     1141 aagcagctgc catgggcttg aaatcgggtg tggattccga ttgcggcggc gtttatcaga
     1201 atcacattca gggagcatta aaaaatggat tactgaccat cggcgatatt gaccgcgcac
     1261 tgatcaatct ttttaccatc cgcatgaggt tgggcgaatt tgatccgaaa gcgaaagttc
     1321 catttgccgg tataaaaccg ggtattatca atgacccgtc gcaccataat ttggctattg
     1381 aaattgccga aaaaacaccg gtgcttttaa aaaacaacaa actggctcaa tccggcgaaa
     1441 atgcattgcc aattaatctg acaaagataa ataaaattgc tgttttggga cctcaggcag
     1501 ataaggtgga gttgggcgat tattccggtc cggtcgaacc ggagttgcga attacacctc
     1561 tccagggcat tcggagttac attgcaaaca acaacctgag tacggaagtt attacccgtt
     1621 cgggtggaaa taccgaccgt cgcaccgact ttttttcaat gaccggtttt tctaccattt
     1681 cagacggaaa agttaagatg gattttgacg caacaaaatt tgacgaagcg gcaaacggag
     1741 tcatcgcttc ttaccggttt gggcaaaacg ttttgcaggg aatcaaggac ggcgactgga
     1801 ctttatacaa aaacgtggat atatcaaatg tggattccat ccggttgaac acgggtgttt
     1861 cgggtgatgg tggtattctg gaaataagag ttggttctgc ctccggcaat attctggctt
     1921 cgcaggaaat caaaccgttg caaaaccgtg gaaatttaag aaattcgggt agaaatcaaa
     1981 ttattccggt aaaaataaac aacctgggaa tttcaggacc gcaatcattg gttttggtct
     2041 ataaagaagc tcccggctcc ggcattgatc aggaaacgct tgatatggca gcctcggccg
     2101 atgtcgcatt gatttttgta ggtactgatc aatccacagg tcgcgaagaa tccgaccgtt
     2161 tttcactaaa acttcccgga aaccagaatg aattaattca ggctgtatcc gcagtcaatc
     2221 ccaatactat cattgtcatg cagactatgg gcatggtgga agtggagcag ttcagaaatc
     2281 tgccaaatgt ctccggtatt atatggacag gctacaacgg tcaggcacag ggcgaggcca
     2341 tggctaaaat tctcttcggc gatgtaaatc cgggtggaaa actcaacgtt acgtggcata
     2401 aatccctcag cgatctgcct gatttgaaca attatacgct tcgcggcgat ggctctaacg
     2461 gaagaactta ctggtactac aacaaggatg tatcctacga atttggcttt ggaatgtcct
     2521 acaccacgtt tgaatatagt aatttcagaa tcaataaaac ggaaatcaca cccaacgaca
     2581 aagttactgt cagcgtggat attaccaatt ccggaaaaat ggatggcgac gaagtggtac
     2641 agatttatgt gaaaactccg gacagtccct catcgctgga gcgaccggta aaaaggctga
     2701 aaggcttcaa acgggtaacc attccccgcg gacagtccaa aaccgtttcc atcgaaatcg
     2761 actgtgccga tctttggttt tgggatgctg aaaatgacaa aattacattt gatcagggaa
     2821 aatatatttt cgaaatcgga gcatcttcca aagacatccg tggaagtgtg caggctaatc
     2881 tgagcggtac gtacaaacct gtgctgacaa ccgttgtggc agaaagcgac ctgtctgtac
     2941 tgaaacccgg aaaaacaata gagacttctc tttcagcagc cttgtcggat gatagttttt
     3001 tagatatttc caaagcaaaa gtaagttaca ggtgcaataa tccttcggtc gctgctgtgg
     3061 atgcaaacgg gaaagtaacg gctgtaggag ttggtgtggc taccattttt gcagatgtga
     3121 cagttgacgg aacgactgtt tcgggcagtt atccgataaa agtgatgccc gatcttactc
     3181 caaaatcaat ctctgtgaat ggaaaaccgg tacctgattt ttccaaagac accaaagctt
     3241 acagctacct gctcgacaaa aaatcgaaaa ttcctgaggt taaagctgca gccatgtttc
     3301 cgggaattcg ggtagatatc gaacaagcag aaagcgtacc cggaacagcc attgtaagga
     3361 tgattgacga tgttacctca gaaataaaca cctattattt gaattttgat gtaaaatccg
     3421 ccggtgatga attcaacgga aatacattag gcaatcaatg gcaatggatt cgggaagatc
     3481 cgctgacaaa ttcgctttct gaaaaatcgg gttctctgac tattaccagc cagcccggag
     3541 atgtgtcaga acgcaccaac aatgccaaaa acatcctcct gcaaagcacc aacaacgatt
     3601 ggaccatcga aacaaaactg actgcatcga gagtgccttc acagcccgaa aatgcaggaa
     3661 ttctggttta tcaggacgat gataattttg taaaactgat gttccgcgct gttgtgaaaa
     3721 cctctcagat gggcagaccg ggcgctccgg ttcaagctgg aaccatcgat ctgatgatag
     3781 aagaaaacgg tatttctaaa tcatttgcca ctttcaatgt gccgaaccct gtaacagatg
     3841 gcaatccgct cattctcaga ctggaaaaga aaggaagtct ctacacagcc tattattcat
     3901 tggatggaaa caagtttgac atgctcggct cggcagttgc attgcttaaa aatatccgct
     3961 ccggattgat agcttgcgac ggaattatta cgcagtacat gaaaagcatt ttctggttcg
     4021 attcggatac caccaagccc gattctcctt tcaatgtctc gtttgattat ttcagaatta
     4081 ctaataatgg tttgaaaacc aaataaatac aaatatcatg aaaaaattaa attcttttct
     4141 ctttctaact ttttttgccg gtacattttt cggattcatg cagataaatg cacaggtggt
     4201 ggaagatttc aagacttcca aaacgactca gattggtaaa aattttccgc aggtaaactc
     4261 tgaaggaaga gtgcgtgtgc aaatatcagc accggatgcc aaactggtgc agctcgatat
     4321 tggcgctgtc aaatacgatt tgaaaaagga tgaaaacgga gtctggaccg gtgagtcagc
     4381 tcctcaggat gaaggttttc actattacca gttaaacatc gacggtgctt cggttcccga
     4441 tccgggcagt ttgtactact acggtgcttc cagatggggc agcggaattg aagttccggc
     4501 acacgatcag gatttttatg ctttaaaaaa tgtacctcac ggacaggttc gggagcacat
     4561 ttattactcc gaagtaaata aatccatgcg tcgctgtttt gtttacactc ctcccggata
     4621 cgataaaaac acaaagaaaa ggtatcctgt gctctatctt caacacggtg gcggtgaaaa
     4681 tgaaaccggc tggtcatcgc agggacatgc caatctcatc atggacaacc tcattgccga
     4741 aggcaaagca aaacctttca tcatcgtaat ggacaacggc acctggacaa tgagtggtcc
     4801 ccgaagaacc gaaggcggca cctggccacc tacaggttgg gcggatggat ttatgaacac
     4861 ccttttgaaa gacatcgtac ctatgattga tgcaagttac cgcaccattg ccaatcagaa
     4921 aaaccgcgct atggctggtc tttcgatggg tggcatgcag acccgcgtga ttactttggc
     4981 taacccggat gtgttctcac aagtaggcat gttcagcggt ggaagcatca gcccggatga
     5041 tgtgaataaa gcgcccggat tcagagaaaa agtgaaactg ctttttgtga gctacggcag
     5101 ctacgagctg gataatccga gaacgaacat gaacggaggg ccgaaagaaa atacagataa
     5161 actgaaagaa atgggtatga atacgcattt ctatgtttct ccgaaaacag ctcacgaatg
     5221 gcagagctgg cgcaggagtt tgtatcagtt tgctcagt
//
LOCUS       DAORKP010000147         5196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3561_length_5196_cov_8.080529, whole genome shotgun sequence.
ACCESSION   DAORKP010000147 DAORKP010000000
VERSION     DAORKP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_5196_cov_8.080529"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="PLN75_07910"
     CDS             <1..1796
                     /locus_tag="PLN75_07910"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRG04079.1"
                     /translation="PYAAISAFALNPLFLNLPELGAMPANSYMKQQYLPKQAELNALP
                     LISFLEVINFKLQYAREAYLHQKSAFLNDPEFKVFFDKNAFWLVPYAAYCVLRDRFGT
                     NDYRQWQEFAQYDESKIAAFTSSDQSHFDEVGVNYYIQYHLHRQLNDAGLYANEHGIV
                     LKGDIPIGVNRNSVDTWVTPELFHMDMNAGAPPDMFAIKGQNWSLPTYNWEQMKNTGF
                     KWWKQRFAQMSNYFDTFRIDHILGFFRIWEIPASEVEGIMGHLNPSIPIYINEFAEKG
                     IGFDYNRFCKPYITDQILYDFFGDEAAWVKANCLQVEEGWILRLKPEYLHQAYVETLY
                     KNGEISEKVKYGLYDLISNVLFFEVEGTDRTQFYPRYGMHSLRTYAELDEHTKKGLDE
                     LYIHYYYHRQDENWYHSGLDKLPALKRATNMLICGEDLGMMNPAVTSVMKELGILSLE
                     IQRAPKSNKIDFAHPNDAAYLSVVTPSTHDMSTIRGWWEEDRSITQQFYNQQLGHWGE
                     APYFCEWWISRDVILQHLYSPAMWAIFQMQDLLGISEKLRRENPHEERINDPSNSEAS
                     WRYRMHIAIEDLMEEEEFNNELKNFIKQSGR"
     gene            complement(1845..2744)
                     /locus_tag="PLN75_07915"
     CDS             complement(1845..2744)
                     /locus_tag="PLN75_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRG04080.1"
                     /translation="MKDTNKALLFVAITVFSWSTVATAFKIALRDFSHYELLLVATST
                     ALIVFAAVITLQSNWHLVAGITKKQWLSYALTGFLNPFFYYLVLFKSYDLLPAQLAQP
                     INYFWPILLTIMLAVFTHQHIAGVKFIGMIISFAGVVLISLGPGSISGEDVSIAGLLL
                     AFFSAFIWAGFWIMNKKNPQTDNTVNLFASFLFGLIYLLIGALFVDLNLDSAKGMQAS
                     IWSGLFEMAIPFLFFGMALRKTSNPVLINQLCYLSPFLSLFFIHFILKEKIFITTLIG
                     LLLIIAGVIFNEYIAKKQSPSRS"
     gene            complement(2780..4003)
                     /locus_tag="PLN75_07920"
     CDS             complement(2780..4003)
                     /locus_tag="PLN75_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04081.1"
                     /translation="MIHRRQVCLLLFILTTTFGYTQDNVPATINKTDSIEKEILNYTD
                     TQTELITKGRRLLLDRFTAGDLQKVKEVKDYLKNQVENRDYVVLFPFEHWLILYWTKE
                     YTELINLIKNPDAFEYTRVNRIAPQQDILYERIREKSLISISKLTSEIYLSGLSEVDN
                     DFLVLYLNAILSGKSSTITQDKLNDLADEFILKYPQSEYNNYIRENLRFKFVPSKWGF
                     AYEFFSGYGMATGNLTNHFTNNIPIGVAFDVQFKNLSLYLRDYIGFSKLKNDISYPDG
                     TWKAKSQVRVFVPEATLGYVVMDRNRVKLAPFAGIGGVNFSPTEKDISNEPFLKNANI
                     GGFSYMAGFNLDIKFGKDISFNEFNSGVGFGFIRIRYSYCMPRLAEKYDGMTGNMHCF
                     TVGIGAFGRTVKRDK"
     gene            complement(4087..4827)
                     /locus_tag="PLN75_07925"
     CDS             complement(4087..4827)
                     /locus_tag="PLN75_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRG04082.1"
                     /translation="MKHLFLNLLFLFGLPGLNAQKVPDATVILDKVDKNMSAGSQIIT
                     AKMEIGTARTTRTMEMKTWTVGDKKSFTEYLAPAREKGTKMLKLNNQLWIYSPSTDRT
                     IQISGHMLRQSVMGSDLSYEDMMTDKKMNDQYKAEVTGEEIIDGRKCLILTLTAIVTD
                     VNYYSQKVWVDEERYVLLKAQLFAKSGKLLKQILFSNVEKVQNRWYPKVFMYKDMLKE
                     GKGTKMTVTSIQLDAGIPESVFNKANLK"
BASE COUNT         1524 a         1099 c         1084 g         1489 t
ORIGIN      
        1 tgccctacgc agccatatca gcttttgcgc tgaatccgct ttttctaaat cttccggagt
       61 tgggggcaat gcctgctaac agctacatga aacagcagta cctgcccaaa caggcagagc
      121 tgaatgcact tccgcttatt tcattcctcg aagttatcaa tttcaaactt caatatgcca
      181 gagaagccta cctgcatcag aaatctgcat ttctgaacga tccggaattc aaggttttct
      241 ttgataaaaa cgctttctgg cttgttccct atgctgctta ctgtgtgctg cgcgatcgtt
      301 tcggaaccaa tgattaccgg cagtggcagg aatttgcaca atacgatgaa tcgaaaattg
      361 cagcatttac gtcatccgat cagtcccatt tcgacgaggt cggtgtaaat tactacatac
      421 agtatcatct gcacaggcaa ctcaacgatg ccggcttgta tgccaacgaa catggaatag
      481 tgctgaaagg cgatatcccg ataggtgtaa accgcaacag cgtggatacg tgggtcacac
      541 ccgaactgtt tcacatggat atgaacgccg gagctccgcc cgatatgttt gccatcaaag
      601 gtcagaactg gtcattacca acctacaact gggaacagat gaaaaatacc ggcttcaaat
      661 ggtggaaaca acgctttgca caaatgtcga actatttcga cacattccgc atcgaccaca
      721 tactcggatt tttccgcatc tgggagattc ctgccagtga ggtggaaggc attatgggtc
      781 atctgaatcc ttctattcca atctatatca atgagtttgc cgaaaaagga atcggatttg
      841 actacaaccg cttctgcaaa ccgtatatta ccgaccagat tctgtatgac ttctttggcg
      901 acgaagcagc ctgggttaaa gccaattgcc tccaggtgga agaagggtgg atactgagac
      961 tgaaacccga atacctccat caggcgtatg ttgagacact ttacaaaaac ggcgaaattt
     1021 ctgaaaaggt aaaatacggg ctctacgatt tgatttccaa cgtcttgttt ttcgaagtgg
     1081 aaggtacgga ccgtacacaa ttttatccac gctacggtat gcattccctc cgtacgtatg
     1141 ccgaactgga cgaacacacc aaaaaaggac tcgacgaact gtacattcac tattactatc
     1201 accgtcagga tgaaaactgg tatcactccg gcctggataa acttccagct ctgaagcgcg
     1261 ctaccaacat gctcatctgt ggcgaagacc ttggaatgat gaatccggcg gtaacctctg
     1321 tgatgaaaga gctcggaata ctgagtctcg aaattcagcg tgcgccgaaa agcaataaaa
     1381 ttgattttgc acatccgaat gatgctgctt acctgtcggt tgtaacacct tcgactcatg
     1441 atatgagtac catacgaggt tggtgggaag aagaccgcag catcacgcag caattctaca
     1501 atcagcaact cggccactgg ggtgaagcgc cgtatttctg cgagtggtgg atttcccgcg
     1561 atgttatcct gcagcacctt tattcaccgg ctatgtgggc aattttccag atgcaggatc
     1621 tgctcggaat atccgaaaaa cttcgtcgcg aaaatccgca cgaagaacgc atcaacgatc
     1681 catccaactc cgaagcaagc tggcgttaca gaatgcacat agctatcgag gatctgatgg
     1741 aggaagagga gttcaacaac gaactgaaaa attttatcaa acaatccgga aggtaatttt
     1801 tcgccgttaa ggattcgaaa aacaaacaat ttcccccgaa cgcattatga gcgtgagggg
     1861 gattgttttt tggcgatata ttcattgaaa atcactccgg caatgatcag aagcaggcca
     1921 atgagcgtag tgatgaaaat tttctccttc agtatgaaat gaataaaaaa cagcgaaaga
     1981 aaaggggaca ggtaacacag ctgattgata agaaccggat tgctggtttt tcgcaaagcc
     2041 attccgaaga acaggaacgg tattgccatc tcgaaaagac ctgaccagat acttgcctgc
     2101 attccttttg cagaatcaag attcaggtcc acaaacaatg caccaatcag caaatatata
     2161 agtccgaaaa gaaaactcgc gaacaaatta acggtgttgt cggtttgagg attttttttg
     2221 ttcattatcc agaaaccggc ccagataaat gcactgaaaa atgccagcag tagtcctgca
     2281 attgaaacat cttcgcccga tatgctgccg ggtcccagtg atataagtac cacgccggca
     2341 aatgaaatga tcatgccgat gaatttcact cctgcaatgt gttggtgtgt gaaaaccgcc
     2401 agcataatgg tgagcaggat aggccagaaa taattgatag gctgagccag ctgagcgggt
     2461 agcaaatcat aggatttgaa cagaaccagg taataaaaaa acggattcag aaatcctgtg
     2521 agagcatagc taagccattg tttcttggtg ataccagcta ccagatgcca gttggattgc
     2581 agggtgataa ccgcagcgaa cacaatcaga gcagttgaag tagccacaag cagcaattcg
     2641 tagtgtgaaa aatcccgtaa agcaatttta aaagctgttg ctactgttga ccagctgaaa
     2701 acagtaatgg caacgaaaag aagtgctttg ttggtgtctt tcatctgttt gtcattcgtt
     2761 aaaaaactaa caaatttgat tatttgtctc tttttacagt tctgccgaaa gcaccaattc
     2821 ctactgtaaa acaatgcata tttccagtca taccgtcata tttttcggct aatcggggca
     2881 tgcagtagct gtagcgaatc cggataaatc caaatccgac tcctgaatta aattcgttaa
     2941 aggaaatatc ttttccaaac ttgatatcca aattaaatcc cgccatgtat gaaaatccac
     3001 caatatttgc attttttaga aaaggttcat tggaaatatc tttttctgtt ggactgaaat
     3061 tcactcctcc aattccggca aacggtgcca gttttacgcg gtttctgtcc attaccacgt
     3121 atcccaaagt ggcttcagga acaaaaacac gaacctggga ttttgctttc catgtcccat
     3181 caggatatga tatgtcattt tttaacttac tgaaaccaat gtaatcccga aggtataaac
     3241 tcagattttt aaattgcaca tcgaaggcaa ctccaatcgg aatgttgttg gtgaaatgat
     3301 tggtgagatt acctgttgcc attccatagc cgctgaaaaa ttcgtaggca aatccccatt
     3361 ttgaaggtac aaatttgaaa cgtaaatttt cacgtatata gttattgtat tcactttgag
     3421 gatacttcag aataaattcg tcggccagat cattcagctt atcttgtgta attgtgctgc
     3481 ttttacctga aagtatggca ttcagataca aaaccagaaa atcgttgtca acttccgata
     3541 accccgataa ataaatttcg gaggtcagtt tgctgatcga aatcaacgat ttttctctga
     3601 ttctttcgta caaaatatct tgttggggag caatcctgtt taccctggta tactcaaatg
     3661 catccggatt tttgattaaa tttatcagct ctgtatattc tttggtccaa tacaaaatca
     3721 gccagtgttc aaatggaaac aaaacaacat aatcccggtt ttcaacctga tttttcagat
     3781 aatcctttac ttcttttact ttttgcaggt cgccggctgt aaatcggtcg agtaacagac
     3841 gtcgcccctt ggtaatcagt tcagtctgag tatccgtgta attcagaatt tccttttcaa
     3901 ttgagtcggt tttgtttatt gtggctggta cgttgtcttg agtatagcca aaggttgttg
     3961 tcagaataaa aagcagtaaa cagacctgtc tccggtgtat catattcttt tgtttttttt
     4021 aaaaatagga ttaatcagat gctatttaga cctgaaaaaa tgattataca aagataatca
     4081 aaaaaattat ttcagatttg ccttattgaa aacgctttcg gggatgccgg catcaagttg
     4141 aatggacgta acggtcattt tggttccctt gccctctttg agcatatctt tgtacatgaa
     4201 aacctttgga taccagcggt tttgaacttt ttctacattt gagaataaaa tctgtttgag
     4261 aagtttgccg ctttttgcaa agagttgagc ctttagcagc acataccgtt cctcatccac
     4321 ccatactttc tgagaataat aattcacatc ggtaactatc gctgtaagtg tcagaataag
     4381 acatttacgt ccgtcaatga tttcctctcc ggtcacttcg gccttgtact gatcgttcat
     4441 ttttttgtcg gtcatcatgt cttcgtatga aaggtcgctc cccatcaccg attgccggag
     4501 catatggccc gaaatctgaa ttgtgcggtc ggttgaaggt gagtaaatcc agagttggtt
     4561 gttcagtttg agcattttcg tccctttttc gcgggcggga gccaggtact ccgtgaaaga
     4621 tttcttgtcg cctacagtcc atgtcttcat ttccatggtg cgggtggtgc gtgcagtgcc
     4681 gatttccatt ttggcagtaa taatctgaga accggcactc atgtttttat ctactttgtc
     4741 cagaatgaca gttgcatcag gcactttctg agcattcaga ccgggtaaac cgaaaagaaa
     4801 taaaaggttg agaaataagt gtttcatagt ttataaaaat taaacccccc taaatccccc
     4861 aaggggggac ttttggtgag tgctgaattc aagatatctt gatgataatg tatttgtgta
     4921 aattttaatt ttatgtatta ttgtccgata aaaacaaaag aaaaaataat gtcgctccta
     4981 cggagctttt gttgatgggt gtaatctatt ttctaccata atgtcgcact gctggtgctg
     5041 aaaattaaag cctcgtagag gtgacattgt ggcaagtcac gttttataag gacttacagg
     5101 aattttctaa aatgtttacc atacaataat aaattttatg tataaattga tttgaaatta
     5161 cgtttcttta agtcccccaa gggggattcc ccgata
//
LOCUS       DAORKP010000148         5157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3626_length_5157_cov_7.829871, whole genome shotgun sequence.
ACCESSION   DAORKP010000148 DAORKP010000000
VERSION     DAORKP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_5157_cov_7.829871"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1200
                     /locus_tag="PLN75_07930"
     CDS             1..1200
                     /locus_tag="PLN75_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRG04083.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLAEEKRYDVYTAVANTGGFSDEE
                     LKVIEEKAYRLGAVKHVAIDVTQEYYEKSIKYMVFGNVLRNGTYPISVSSERIFQAIA
                     IINYAKEINAEYVAHGSTGAGNDQVRFDLTFQILAPEIKILTPTRDLTLTREYEINYL
                     KEHGYVADFKKMEYSINKGLWGTSIGGKETLKSEQTLPEEAYPGQVVNTGEETVTIGF
                     EKGEICSVNGEITNDKIHLINKLENIANKYAIGRDMHIGDTIIGIKGRVGFEAAAPML
                     IINAHKMLEKHTLTKWQQYWKDQLGNWYGMFLHEAQYLEPVMRDIEQFLESSQQNVTG
                     TVIIKLRPYSYTLVGVESDFDLMKTDFGEYGEVNKAWTADDVKGFTKILGNQMKIFYN
                     VQKRNGK"
     gene            complement(1283..2683)
                     /locus_tag="PLN75_07935"
     CDS             complement(1283..2683)
                     /locus_tag="PLN75_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HRG04084.1"
                     /translation="MKLFFLIYFFFLSLIAFPGNDSIRYEVGIADVASSGEFAPFWLQ
                     HNRYGKISPSPSSGMISGAIEKKFDKPHKLFDYGFKANALVRYDSNKNLDIFFQELYA
                     KARFSVFNLSVGAREQIFGNQDSTLSSGGLLFSENTRPMPEISAGIERFTPVPLTFGI
                     VEIKGGLRHALFTDNIYTKGILLHHKFGYLRMGGKLPVHLEYGIEHAAQWGGTDPVHG
                     PNPSGFKNFTRIFRAESGGKDATLSDQINILGNHIVSQGLKLEAEAAGWKLSGYWQNL
                     SEDPPVVFITANRMNIQDGLWGISLKNKSFPYIKGVLYELLNTTDQSGPYHDKDGLIY
                     GGADNYFRGAYPEGWSYYSRTIGTPFVTSPLYNNNRVLSTQNNRVRVHHFGLEGSVKG
                     FEYRALASFSRNYGVLGSQIDIPNNSFLLEINKHITWLSGFDISLSAAGDWGKYYGNS
                     QGVLISIRKKGILLDY"
     gene            complement(2736..3611)
                     /locus_tag="PLN75_07940"
     CDS             complement(2736..3611)
                     /locus_tag="PLN75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRG04085.1"
                     /translation="MPGNGRASAVSFAVNGKGYIALGRDANSVQLNDCWEYDPSTDSW
                     QQKSSFPGIARVKATAAILNGRAYIGLGHNNYSGVYNSNSCLKDFWVFTPETDSWRKL
                     ADFPSNFTDACVSFVYNDEIVVANGFSESGYGGEIWKYRPDDDRWYRLKNFEGQWRFG
                     GVLCANEQHIFFGTGFGIGNLNDWWEYLPQSDSWKKLRAMPDKGRVNGVSLTIDNRYF
                     VATGRHFGGNIGRGHLKSDVMEYDPRKNSWHKAGELPDGNRENAVAFVIDGRGYIGLG
                     ENDNKVLNDFWSFEP"
     gene            4943..>5157
                     /locus_tag="PLN75_07945"
     CDS             4943..>5157
                     /locus_tag="PLN75_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04086.1"
                     /translation="MKKVFQINLKSITKVFAVVMALSFVTFTGCKSYDQDIDALNTDL
                     ASLKTELTNLNNATKSALETQIAALNT"
BASE COUNT         1633 a         1054 c         1010 g         1460 t
ORIGIN      
        1 atgaaagaaa aagtagtttt agcattcagc ggtggattgg acacttcatt ctgcgtaaaa
       61 tatttagccg aagaaaaaag gtacgatgta tatacggctg tagccaatac cggcggattt
      121 agcgacgagg agctgaaagt gattgaagaa aaagcctacc gactgggagc tgtaaaacac
      181 gtggcaattg atgtaaccca ggaatattac gaaaaaagca tcaaatacat ggttttcggt
      241 aacgtgctgc gcaacgggac ttaccccatt tccgtaagct ccgagcggat tttccaggcc
      301 attgccatca tcaattatgc caaggaaata aatgcagaat atgtagccca cggaagtaca
      361 ggtgcaggta atgatcaggt gcggttcgat ctgacctttc agattctggc acctgaaatc
      421 aaaatcctta ctcccacacg cgacctcacc ctgacccgcg aatatgaaat caattacctg
      481 aaagaacacg gctacgtggc tgacttcaaa aaaatggaat attccatcaa taaaggactg
      541 tgggggacat ccatcggtgg taaggaaacg ttgaagtccg aacaaacgct gcctgaagaa
      601 gcatatccag gtcaggtagt caatacaggg gaagaaaccg tgacgatagg ttttgaaaaa
      661 ggtgaaatct gttcagtaaa cggtgaaata acgaacgata agattcactt aatcaataaa
      721 ctcgaaaata ttgccaataa atatgctatc ggccgcgata tgcacatagg cgataccatt
      781 attggcatca aagggcgcgt gggttttgaa gcggcagctc ccatgctcat catcaatgcc
      841 cacaaaatgc tcgaaaaaca tacacttacc aaatggcagc aatactggaa agatcagctg
      901 ggcaactggt acggcatgtt tctccacgaa gctcaatacc tcgaaccggt gatgcgcgat
      961 atagagcaat tcctcgaaag ttcacagcaa aacgttaccg gaacggtgat tatcaaactc
     1021 cgcccctaca gctacacact cgtgggagtg gagagtgatt ttgatttgat gaaaaccgat
     1081 ttcggtgaat acggcgaagt aaacaaagcc tggacagccg atgatgtgaa aggatttacc
     1141 aaaatactgg gtaatcagat gaaaattttc tacaacgtgc agaaaagaaa cggaaaatag
     1201 ttatcccata gatacttaaa acgcaaaaaa gaactgaaaa agtttttcgg agattccaaa
     1261 tccggagaac ttttttttta gtttaataat ccagtaaaat cccttttttt cggatggaaa
     1321 taagtacgcc ctgcgagttt ccgtagtatt ttccccaatc acctgcagcg gataaactga
     1381 tgtcaaatcc ggataaccag gtgatgtgct tatttatttc cagtaaaaaa gagttgttcg
     1441 gaatatcaat ctgcgaacca agtactccgt aatttctgct gaacgaagcc agtgcgcggt
     1501 attcaaatcc tttcacagag ccttccaaac cgaaatgatg tacacggaca cggttgttct
     1561 gagtactcag cacccggttg ttgttataaa gcggcgatgt gacgaaagga gtaccgatgg
     1621 tgcgcgagta atagctccac ccttcgggat aagcgcctct gaaataatta tcggcaccgc
     1681 cgtaaatcaa accatccttg tcgtgatacg gtcccgactg gtcggttgtg ttcagcagtt
     1741 cgtacaatac acccttgatg taaggaaatg atttgttttt cagggagata ccccaaagtc
     1801 cgtcctgtat attcatccgg ttggctgtaa taaacacgac gggaggatct tccgaaagat
     1861 tttgccagta tccgctgagt ttccatccgg ctgcctccgc ctccagtttc agtccctgcg
     1921 aaacaatgtg attccccaaa atatttatct gatcgctcag ggtcgcatcc ttgcctcccg
     1981 attcagcacg aaaaatccgg gtgaaattct taaatccgga aggatttggt ccgtgaacag
     2041 ggtctgtacc tccccattgt gccgcatgtt caatcccgta ttccagatga accggaagct
     2101 taccacccat gcgcaggtat ccgaacttgt gatgaagcaa aatccctttg gtataaatat
     2161 tgtcggtaaa gagcgcatgc cgcaaaccac cttttatttc tactattcca aaggtgagag
     2221 gaaccggtgt aaaccgttcg ataccggccg aaatttcagg cataggccgg gtgttttccg
     2281 aaaaaagcaa accacctgat gagagtgttg agtcctgatt gccaaaaatc tgctctctgg
     2341 cgccaaccga aaggttaaaa acagaaaaac gggctttcgc gtatagttcc tgaaaaaaaa
     2401 tatccaaatt tttattgctg tcataacgca ccaatgcatt ggctttgaat ccgtaatcga
     2461 acagtttgtg gggcttgtca aatttctttt caatagctcc ggatatcata ccgctcgagg
     2521 gcgatggcga gatttttcca taccggttgt gttgcagcca gaatggagca aattcgccgg
     2581 atgaagctac atcggcaatt ccaacttcat atcttattga atcatttccg gggaaagcaa
     2641 taagcgacag aaagaaaaaa tatataagaa aaaacagctt caaaaaaccg ttttttgatt
     2701 tattttttgt cgaagaggat ctcatttttg ttgttttaag gttcgaaact ccagaaatcg
     2761 ttcagtactt tattatcatt ctcgcccagt ccgatatatc cccgtccatc aatgacaaaa
     2821 gcaactgcat tctcgcggtt tccgtcgggc aattcacctg ccttatgcca tgaattcttt
     2881 cgcgggtcgt attccatcac atccgatttg agatgccccc ggccaatatt cccaccaaaa
     2941 tgtcggcctg tggcaacaaa ataccggtta tcaatggtga gcgaaacacc attcacccgg
     3001 cccttgtcgg gcattgccct gagttttttc cagctgtccg attgaggaag atactcccac
     3061 cagtcgttca gatttccgat tccgaagccg gtgccgaaaa atatgtgttg ctcgttagcg
     3121 cagagaactc cgccaaaccg ccattgacct tcaaaattct tcaaccggta ccacctgtca
     3181 tcatccggac ggtatttcca gatttcacct ccgtaacccg attcgctgaa tccgttggcc
     3241 accactattt catcgttata cacaaagctt acgcaagcat cggtaaagtt gctcggaaaa
     3301 tcggccaact tcctccagct gtcggtttcg ggtgtgaata cccagaaatc tttcagacac
     3361 gaattcgaat tatatacacc cgaatagttg ttgtgcccca gaccaatata agccctgcca
     3421 ttcagaatgg cagctgtggc ttttaccctt gcaattcctg ggaaggatga tttttgctgc
     3481 caggaatcgg tggaaggatc atactcccaa caatcattga gctgaacact gtttgcatcg
     3541 cgtcccagtg cgatgtaccc tttcccgttc actgcaaatg aaactgcaga agcccgtcca
     3601 ttgcccggca tggaagtttt ttttacaaaa ttcaccgtca attcaggtgg atcagcctca
     3661 caaccggttg ttagaaggac agatattgat aataatatta atgtgtatat taaaactgat
     3721 attttcattt ctcagaattt caaactgatt aataaactat aattccattt caggtgttat
     3781 tatcccgtat aatgtttcaa atacattttt ttttatttct gttgaaaaaa cggatttgca
     3841 gcaacaaaat taatgttttt atcgttaata agcatgtata ctgccaaaaa cgtaccgaat
     3901 cagtaatgaa acggatattt ttcaggtttt taaaggttgg atttaataca cggatttttc
     3961 gttttgagga atcactatgc aaaatctacg aaaaacagac atataatatt atagcacgct
     4021 gaaaaagatg gatttaatat caaatgtcaa aaaaagtttc gatactataa tttttcggaa
     4081 tgatttttgt tggaagtgat aggttgtgat tctccttgcc ggagtattaa cttttcattt
     4141 cccttttttc ggcttttcct gaatgaaaag tcaatatttt caataaaaat catttttcag
     4201 gggtgtaaaa tgcagaaata ttttataaaa cacttgcaaa ttgaaaaaaa tagtataaat
     4261 ttgccagact ttttgttgca aattgaatgg attagataat tcagaatgtc aatgttataa
     4321 cttatacatt caataatgtt taaattgtga ttaataagac ttcaatttac tgattcagat
     4381 ttatgtggac gatagataat ttatcttatt ttataaaaaa atacgtaaaa tattttgaaa
     4441 tccgtttgaa tgttatatct ttgcgctaaa ttattgctaa ctcccctgct taaaagagtt
     4501 tatcaggggg ataatttata agggcatgac aaaaacttat ttgaaaagtt aatgtgtttt
     4561 aataaaaaag aagtaaaatt attttgaatt ccatttgaat gttttatctt tgcagttatg
     4621 caagtcaaca ttgtgttttg tgtggataca attaataagt aacaataccc tgcaatacca
     4681 ggaacaaaac ctgaaacaaa agtcaattgt ttgttttttt ttgtcgatgg aaataaataa
     4741 ttactttttt ttcgcaatat aatttaatac gaaagagtta aaaaaccaca agtaaatatt
     4801 taaaaactaa ttatttatga ataaaatcta atcaattctc tcattttcac gggcaggtag
     4861 ccctgtggaa attattgctg tacaatgaat ccagtaacaa atagtaacta taaaaaaaaa
     4921 ttaattatca aaaaaaaaat ttatgaaaaa agtatttcag attaacttga aaagcataac
     4981 caaagtcttc gctgtggtta tggctctttc atttgtaaca tttacaggat gtaaatctta
     5041 cgatcaagac attgacgctt tgaacactga tcttgcaagc ttgaagaccg aactcacaaa
     5101 tctcaacaat gcaacgaaaa gtgctttaga aactcaaatt gcagcactta acacaga
//
LOCUS       DAORKP010000149         5155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3628_length_5155_cov_9.673922, whole genome shotgun sequence.
ACCESSION   DAORKP010000149 DAORKP010000000
VERSION     DAORKP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3628_length_5155_cov_9.673922"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1669
                     /locus_tag="PLN75_07950"
     CDS             278..1669
                     /locus_tag="PLN75_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRG04087.1"
                     /translation="MKNKYYDLIEQTFEFPNDEFTVEDNELYWHGIPLMDIIKQYGTP
                     LRISYLPRITEAIQKARRMFNVAMAKVDYEGTYHYCYCTKSSHFSFVMEEVLKNGAHI
                     ETSSAFDINIIETLYEQELLTPQTFVLCNGFKRPMYVENIQNLIKRGFTNVIPILDNI
                     FELDLLMNDFEEKFKVGIRIASEEEPKFDFYTSRLGIRYNDIIPYYEEKIATNEQVEL
                     KMLHFFINTGVKDTLYYWNELSKAVNLYCELKKVCPTLDSLDIGGGFPIQTHMDFTFD
                     YEYMAEEIVNQIKLICNQNGVPEPHIFTEFGSFTVGESGAMLYSIVNQKKQNDRELWN
                     MIDSSFMTTLPDTWSINQRYVFLAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAVF
                     LPKMQLGREQYIGFFHMGAYQESLSGFGGIQHCLIPAPKMVIIDKDEDGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(1739..2719)
                     /locus_tag="PLN75_07955"
     CDS             complement(1739..2719)
                     /locus_tag="PLN75_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRG04088.1"
                     /translation="MKKKSNILFLLMVAVLLHVFLTSCDRRPEGVLNSRSMENLLVEM
                     HLLEGSLRASGGYYGSGTDKMEYYNALFDKYGITVDEFDSSLVWYTKHPKQFERIYLN
                     VVGRIDTLSAEILRGKYHPVDSSARSGEMNLWTKATKYVFTKDSARNKLEFKIENVEL
                     LPGDVYKLSFLRRSAPSDSSRNPHAVLYVNYANDVVDSIYTRTPNDSVLRRYTLIFKA
                     RKNLPVRSLSGYLLGTESSAGKMNASVDSIRLIRRYNPFKQDSIRAAMHKMDSTLVVA
                     DSLQTAAADSSKSLRKDTLKSVPGDTALPKKLIPGKGKRLQQVRKLEAPQ"
     gene            complement(2703..3317)
                     /locus_tag="PLN75_07960"
     CDS             complement(2703..3317)
                     /locus_tag="PLN75_07960"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRG04089.1"
                     /translation="MSIKTKSILLIILLLLIDQASKIYIKLNFALGDEVEVFPWFKIL
                     FIENNGMAFGMELVGKLFLTLFRITAVVFLSYFISKLIRQNARQGYILAISMLTAGAA
                     GNIIDSVFYGVIFNESTPYQVATLFPEGGGYASLFYGKVVDMLYFPLITNAGGETVFF
                     RYIFNVADSCVTVSVFIILLFFRKDLNHSLESKKTPETSHEEKE"
     gene            complement(3437..3823)
                     /locus_tag="PLN75_07965"
     CDS             complement(3437..3823)
                     /locus_tag="PLN75_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRG04090.1"
                     /translation="MTQKEKTRYTDKELEEFRQIILEKLEKAQKDYELLRTGLNNSDG
                     NDVSDTSPTFKVMEEGASTLGKEETGQLAQRQMKFIQHLQAALVRIENKTYGICRETG
                     KLIPKERLRAVPHATLSIEAKEGSKK"
     gene            complement(3918..>5155)
                     /locus_tag="PLN75_07970"
     CDS             complement(3918..>5155)
                     /locus_tag="PLN75_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRG04091.1"
                     /translation="AADAEIPVSERPEIDRWIISLLNTLVKDVKSYYEDYEPTKAGRA
                     ISDFVGENLSNWYVRLNRKRYWGGEYDKDKIAAYQTLYTCLVTVAQLMAPIAPFYADQ
                     LYLDLTAPHPPQGGASEAYLNVSENVEMPVSSPLSGELEGASVHLTDFPEFDEALIDK
                     NLEECMQLAQQISSMILALRRKAEKKVRQPLSKAVIPAPDEKTYEQIRYVADLIKAEV
                     NVKQLEIISADADMDSLVKRVKPNFKTLGKKYGKQMKEIAAALPGMTKQQIAQIEQKG
                     EYILTLPSGDVILTPEDVEIFTEDMPGWLVANEGNLTVALDIDVNEALQREGIARELV
                     NRIQNIRKSSGFEIVDKIDIQIQKQDQINAAVTEFSEYIAAQVLASSIALVDAAENAA
                     ELDFEDFTVKIVVAKTIIS"
BASE COUNT         1329 a         1181 c         1178 g         1467 t
ORIGIN      
        1 gctaactcga agttagcagt taattattct tgaagttagc tgttaattat tctcgaagtt
       61 agcagttaat tatttctgaa gttagttgtt actttaacaa ccgtaaagct gaattgtttt
      121 gaaaatattc gtttcataca atccgttatt attcaaagta tttgtatatt tgcaccctgt
      181 ttgcacaaaa aaatcaaatc cgcgttattt actttgattt ttttcagcgc atccggatta
      241 tttacatgga ttattcatct ttattttaag caaagaaatg aaaaataagt attacgatct
      301 gatcgaacaa accttcgaat tcccgaacga tgaattcacg gtggaagaca acgaacttta
      361 ctggcacggc atcccgttga tggacattat caagcagtac gggactccgc tgcggatatc
      421 gtacctgccc cgcattacgg aagctattca gaaggcacgg cgtatgttca acgtggcgat
      481 ggccaaggtt gactacgagg gcacgtacca ctactgctac tgcacaaaga gttctcactt
      541 ctcattcgta atggaagagg tgctcaaaaa cggcgcccac atcgaaactt cgtcggcatt
      601 cgacatcaac atcatcgaaa cactctacga acaggagctg ctcaccccgc agaccttcgt
      661 gctgtgtaac ggattcaaac gcccgatgta tgtggagaac atccagaacc tgatcaaacg
      721 gggattcacc aacgtgattc cgatactcga caacattttc gaactcgatc tgctgatgaa
      781 tgattttgag gaaaaattca aggtgggtat ccgcattgct tcggaagaag agccgaaatt
      841 tgacttttac acctcgcggc ttggtattcg ctacaacgat atcatccctt attacgaaga
      901 aaagatagct accaacgaac aggtggaact gaagatgctt catttcttca tcaacaccgg
      961 agtaaaagac acgctttact actggaacga actcagtaaa gcagtcaatc tctactgcga
     1021 actgaagaaa gtttgtccta cactggacag cctggacatc ggcggaggat tcccgataca
     1081 aacccacatg gatttcacgt tcgattacga gtacatggcc gaagaaattg tgaatcagat
     1141 aaagctcatc tgcaaccaaa acggagtacc cgaaccgcac atttttaccg aattcggctc
     1201 atttaccgta ggtgaaagcg gtgccatgct ctactccatc gtgaaccaga aaaagcagaa
     1261 cgaccgcgaa ctctggaata tgatcgacag ctcgtttatg accaccctgc cggatacctg
     1321 gtctatcaac cagcgctatg tgtttctggc catcaacaac tgggattcgg aatacgagcg
     1381 ggtgaacctg ggcgggctca cgtgcgacag cgaagacttc tacaacgccg aggtgcactc
     1441 caatgcggtg tttctgccca aaatgcagtt gggacgcgag caatacatcg gtttcttcca
     1501 catgggggct taccaggagt cgctgagcgg cttcggcggg atacagcact gccttattcc
     1561 ggctccgaaa atggtcatta tcgacaagga cgaagacggc gaatacttta ccaaactctt
     1621 tgccaaggag caaagctata aatccatgct gaagatactg ggatattaac ggcgtaacgt
     1681 gatgggaaga aaaagaaaag tccgcaggtt tcagtgcctg cggacttttt atttattttc
     1741 attgaggcgc ctcgagttta cggacctgct gtaatctttt ccctttaccg ggaatcaact
     1801 tttttggtaa tgccgtatca ccgggaacag atttcaatgt atctttccgg agagattttg
     1861 atgaatcggc ggcagcggtt tgaagcgaat cagcgacaac aagcgttgag tccattttat
     1921 gcatggctgc ccggatactg tcctgtttga aaggattgta cctgcggata agccggatgc
     1981 tgtccacgct tgcgttcatt tttccggccg aagattcggt gccgagcagg taaccgctca
     2041 gcgacctgac cggaagattt ttccgtgcct tgaaaattag cgtatagcgg cgcaaaacac
     2101 tgtcgttggg tgtacgggtg taaatgctgt cgacaacgtc gttggcatag ttcacataca
     2161 acactgcatg cggattgcgg ctggaatccg aaggggccga gcgacgcaga aacgacaact
     2221 tatacacatc gccgggcagc aattccacat tttcgatttt gaattcgagc ttattacgcg
     2281 ccgaatcttt cgtgaagacg tatttggttg cctttgtcca aaggttcatt tcgcccgagc
     2341 gggcagaaga atccacagga tggtattttc cccgaaggat ttcggcgctc agcgtatcaa
     2401 tgcggcctac cacgttaaga taaatgcgtt cgaattgttt cggatgtttg gtgtaccaca
     2461 ccaggctcga gtcaaactca tccaccgtga tgccgtattt atcaaaaagt gcgttgtagt
     2521 actccatttt atcggttccc gatccgtagt aacctccgga tgcccgcagc gatccttcga
     2581 gcaggtgcat ttccaccagc agattttcca tgctgcggga gttcagtaca ccctccggtc
     2641 gtctgtcgca cgatgtaaga aaaacatgga gcagcactgc caccattaac agaaacagga
     2701 tgttactctt tttcttcatg ggaagtttcg ggagtttttt tagattcgag gctgtggttc
     2761 aggtcttttc tgaaaaacag caggatgatg aatacagaaa ccgttacaca ggaatcggcc
     2821 acgttgaata tatacctgaa gaataccgtt tcgccgccgg cattggtaat gagggggaaa
     2881 tagagcatat caactacttt gccataaaag agggaggcat agccgccccc ttcgggaaag
     2941 agggtagcaa cctgataggg agtactttcg ttgaaaatga cgccgtagaa taccgaatcg
     3001 atgatattgc cggcagctcc ggctgtgagc atggaaatag ccagtatata tccctgacgg
     3061 gcattttggc gtattaattt actgataaaa tacgacagga aaactacagc ggtaatccgg
     3121 aagagcgtaa ggaataattt acctacaagc tccatcccga aggccatacc gttgttttcg
     3181 atgaataaaa tcttaaacca gggaaaaact tccacttcgt cgccaagcgc aaaattcagt
     3241 ttgatataaa tttttgatgc ctgatcgatg agtaaaagca ggatgatcaa taagatggat
     3301 ttagttttaa ttgacatgct ttagttagtt ttctcataca aaaattcgac aaccgttttg
     3361 tgttttcggc tgtcgaatga tttatttttt agtccgtaag ttaacttttt aacaccgaat
     3421 cctttagctt cgtgtgctat tttttgctgc cttctttcgc ttcgatgctc agtgtggcat
     3481 gcggaacggc acgcaggcgt tctttcggta tcagtttacc ggtttcacgg catattccgt
     3541 acgttttgtt ctcgatgcgt acgagtgctg cctgcaggtg ttggataaat ttcatctggc
     3601 gttgtgccag ctgtccggtt tcttccttgc cgagggtgga ggctccttct tccataacct
     3661 tgaatgtggg cgaagtatcc gaaacgtcgt ttccgtcgga attgttcaat ccggtacgca
     3721 gcaattcata atccttctgg gctttttcga gtttttccag aattatctga cgaaattctt
     3781 ccagttcctt gtcagtatat cttgtttttt ctttttgagt cataattcag catgtttatc
     3841 aggttcaaca gtttatttcc taaatgaatg tgcgaaaata cgcatttaat ttcaaaataa
     3901 caattatttt acaaatgtta tgaaattatt gtttttgcca ccacaatctt cactgtgaag
     3961 tcttcgaaat cgagttctgc agcattttcg gcagcgtcaa ccaacgcaat ggaggaggcc
     4021 agaacctgtg cggcgatgta ctccgaaaat tcggtcacgg cggcatttat ctgatcctgc
     4081 ttctgaattt gtatgtctat cttatccaca atttcgaagc cacttgattt tcgtatgttc
     4141 tgtatgcgat tcaccagttc gcgggctatg ccctcgcgtt gcagagcttc gttcacatca
     4201 atatccaggg ctacggtcag atttccttca tttgccacca gccatccggg catgtcttcg
     4261 gtgaatatct ccacatcttc gggtgtgagg attacatcgc ccgatggcag tgtaagtatg
     4321 tattcacctt tttgttctat ctgagcaatt tgctgtttgg tcattccggg cagtgccgct
     4381 gcaatttctt tcatctgttt gccgtatttc tttcccagtg ttttgaagtt cggttttacc
     4441 cttttcacca ggctgtccat atctgcatcg gctgagatga tttccagctg ttttacgttc
     4501 acttcggctt ttatcagatc agctacatac ctgatttgtt cgtatgtttt ttcatccggt
     4561 gcaggtatca cggctttaga gaggggctga cgaacctttt tctcagcttt acggcgtagc
     4621 gccagtatca tggaagatat ctgctgcgcc agttgcatac attcttcgag atttttgtcg
     4681 attaatgctt catcaaattc cgggaagtca gtcagatgaa cagaagcccc ctctaattcc
     4741 cccgaaaggg gagaacttac gggcatctca acattttctg aaacattgag atacgcttct
     4801 gacgctcccc cttgaggggg atggggggct gttaaatcca aatacaactg gtcggcataa
     4861 aacggagcaa tcggtgccat aagttgtgcc acggtaacca ggcaggtata aagtgtctga
     4921 taagcagcga ttttatcttt atcgtactca ccaccccagt agcgtttgcg gttgaggcgt
     4981 acgtaccagt tgctcaggtt ttcacccaca aagtccgaaa tggcgcgtcc ggctttggtc
     5041 ggttcgtaat cttcgtaata cgacttcaca tcttttacca gggtgttgag caatgaaata
     5101 atccagcggt caatttcagg acgttccgaa accggaattt cggcatcagc tgccg
//
LOCUS       DAORKP010000150         5145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3640_length_5145_cov_9.562672, whole genome shotgun sequence.
ACCESSION   DAORKP010000150 DAORKP010000000
VERSION     DAORKP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3640_length_5145_cov_9.562672"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..780
                     /locus_tag="PLN75_07975"
     CDS             34..780
                     /locus_tag="PLN75_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRG04092.1"
                     /translation="MKAMIFAAGLGSRLKPITDSLPKALVPVGGKPLLEHNILKLKQA
                     GFDEIMINIHHFGDQIIDFLAAKNNFDIRIELSDERNELLDTGGGMKKAAYFFDDDRP
                     FLVHNVDILSNVDLKEVYNQHLKDPERLASLVVSYRDTYRYLIFDDSGCLQGWVNIRT
                     GETKPLKNMYILPFQKFAYAGIQVVSPRIFRLMEKEPEKFPIMDFYLQNCITQKIAGY
                     VPENLRMLDVGKLNSLNTAEEFILQNVSMP"
     gene            948..1523
                     /locus_tag="PLN75_07980"
     CDS             948..1523
                     /locus_tag="PLN75_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRG04093.1"
                     /translation="MKTFELSGEVRENLGKKATKAVRVSENIPCVLYGGKDNVHFTVS
                     ASAVRKLIYTPEVYVVELTIGKNKTKAIMKELQFHAVTDKVQHIDFLEIDEKKPVVVE
                     LPVKLTGLAEGVKAGGKLSLEMRKLKVKGIYTNIPESITVDVTNVGLGKSIQVGKLAL
                     KDLEILNSKMSVVAQVKLTRAARGAAANAQS"
     gene            1533..2096
                     /gene="pth"
                     /locus_tag="PLN75_07985"
     CDS             1533..2096
                     /gene="pth"
                     /locus_tag="PLN75_07985"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRG04094.1"
                     /translation="MKYLIVGLGNIGSEYHNTRHNIGFTILDAFAKASNVFFTDNRYG
                     ATAEVKLKGKTLLLLKPSTFMNLSGNALRYWMQKENIPLEHILILVDDIALPFGTLRL
                     KPKGSDAGHNGLKNIQEILGHSNYNRLRFGIGNDFPRGGQVHYVLSNWTDEESALLPD
                     RCEKAGEIIKSFCLAGVQHTMNQYNNK"
     gene            2225..2617
                     /locus_tag="PLN75_07990"
     CDS             2225..2617
                     /locus_tag="PLN75_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRG04095.1"
                     /translation="MEVRIDKWLWAVRLFKTRSLATEACKKGKILIQQAAVKPSRTVK
                     VGDIIQIKRPPVVYSFKVLALAQNRMNARLVPEHMQDITTPDQLELIELGKIAAKTAR
                     ERGTGRPTKKERRDMDDFFEPFFVDDED"
     gene            2705..3256
                     /locus_tag="PLN75_07995"
     CDS             2705..3256
                     /locus_tag="PLN75_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HRG04096.1"
                     /translation="MYIATALRKKNIIEYLLYMWQIEDLIRAFGLDMSRINEKIVEPY
                     QLSDNDKKRLYEWYESLTEMMRTENAQEAGHIQLNKNIVIQLNDFHAEVLQSGMLHDY
                     NSRFNRLRPFLSQLRSKQNDPEISDIELCFNFLYGIMTLRMQKAEITPETLQVKDEIG
                     KFMGLLNEYYQQYVSGELQLQEE"
     gene            complement(3277..4029)
                     /locus_tag="PLN75_08000"
     CDS             complement(3277..4029)
                     /locus_tag="PLN75_08000"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRG04097.1"
                     /translation="MNTFRRLYTVLFVLLLISRFSFGAEWNIIRGEKLKYKIAFSSGL
                     TGNVKGGEATMSVKSSTVRTGNHDTYLATLYGATTGVIEWFYQVENKYETYIDVNSGA
                     PLMYRQAVRENKYRSADTVFFDQNNHVATYRHKRITIPPNTHDFVSMIYYVRSLEASK
                     MKKGDSVTIPFFTSDKVIMSKVVFNGIEQIRANNMLVSCYAFKPQVGKGKVFNQDYPA
                     TIWISTDSKRLPMLIEARMKVGRVKMELLKSE"
     gene            4328..>5145
                     /locus_tag="PLN75_08005"
     CDS             4328..>5145
                     /locus_tag="PLN75_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04098.1"
                     /translation="MKRIQIIVILSVVLVATAATIALAQKSKTSSMETRWKKVERFAE
                     QNLPESALKEVEAILGQARKEKKSPEIIKSMVYKMRFILDKNPDETEKLIAEFEDYTI
                     AGTDPAEKAIMYSMTAELYLKYYQNERYKINQRTELQGFVPEDMKEWTRNIFEDKIVG
                     LACKSLEKPETLQKIPSLQFAAILEQGKDSRDMQPTLFDLLSYRKIDLLMNFKENDSD
                     NEFPEDKADFTSSGQNVLASENQLTIHSKILDTYNGIIDYNKKQGNTPAIVYAEL"
BASE COUNT         1653 a         1071 c         1057 g         1364 t
ORIGIN      
        1 cgtccttaat ttaaaaaaat aaaaaatctt cgtatgaaag caatgatttt tgccgcagga
       61 ctcggttcca gactaaaacc cattacagat tcgttgccca aagccctggt tccggtagga
      121 ggcaaaccat tgcttgagca caacattctt aaactcaaac aagccggatt tgatgaaatc
      181 atgatcaata ttcatcattt cggggatcag atcattgatt ttctggctgc caaaaataat
      241 tttgatatcc gcatcgaact ttcagatgag cgcaatgaac tgctcgatac cggtggagga
      301 atgaaaaaag ctgcgtattt tttcgatgat gacaggccat ttctggttca taacgtggat
      361 attctttcaa acgtggattt gaaggaagtt tacaatcaac atctcaaaga ccctgagcgc
      421 cttgccagcc tcgtggtaag ctacagagac acataccggt atctgatttt tgacgattcg
      481 ggatgtctgc agggatgggt aaatataaga accggagaaa ccaaaccttt gaaaaacatg
      541 tatatccttc cttttcagaa gtttgcttac gcaggcatac aggtagtttc gccccgtatt
      601 ttccggttga tggaaaaaga accggaaaaa tttccgatca tggattttta tctgcaaaac
      661 tgtataaccc aaaaaatagc aggctatgta cccgaaaatc tgcggatgct cgacgtggga
      721 aaattaaact cgctgaatac agcagaggaa ttcatcctac aaaatgtcag catgccgtaa
      781 actgaaactc ccgtaaattc atttcatttc aatttgcata ttcacatcaa aattactatc
      841 tttgcaccac ttttttgcaa aagaaagtgt gatgggaaat taagcgacta atcacttaat
      901 aacgaaacgc ttaaattttg agtaacacaa tttatcatta aaaaaaaatg aaaacatttg
      961 aattatcagg cgaagtaaga gaaaacctgg gaaaaaaagc aaccaaagct gttcgggtat
     1021 ccgaaaacat cccctgcgtg ctttacggtg gtaaagataa cgtacacttt acagtatctg
     1081 caagtgctgt acgtaagctc atttatactc ccgaagtata tgtggtggaa cttaccatcg
     1141 gtaagaacaa gactaaagcc atcatgaaag aattgcaatt ccatgcagtg acagacaagg
     1201 tacagcacat cgacttcctc gaaattgacg agaaaaaacc ggtggtcgta gaattgcccg
     1261 ttaaacttac cggattggcc gaaggtgtga aagccggtgg aaaattaagc cttgaaatgc
     1321 gaaaattgaa agtaaaaggc atctacacca acattccgga gtcaattact gtggatgtaa
     1381 ctaacgtagg ccttggaaaa tcaattcagg ttggcaaact tgcactgaaa gatcttgaaa
     1441 tactaaactc gaaaatgtct gtagtagctc aggtgaaact cacacgtgcc gcacgtggtg
     1501 ctgctgccaa tgctcagtca taatcacagc aaatgaagta cctcattgta gggttgggta
     1561 acattggttc tgaataccat aacacccgac acaacattgg atttacaata ttggacgctt
     1621 ttgctaaagc gtccaatgtt tttttcacag acaaccgtta cggagctact gccgaggtaa
     1681 agctgaaagg aaagacacta ctcctgctga aaccttccac attcatgaat ttgagcggca
     1741 atgccctccg gtactggatg caaaaagaga atattccgct ggagcacata ttgattctgg
     1801 tagatgacat tgctttgcca ttcggaacac tcagactgaa gcccaaaggt tcagatgccg
     1861 gtcacaacgg acttaaaaat atacaggaaa tactgggtca cagcaattat aatcgacttc
     1921 gattcggtat cgggaatgac tttccgcgtg gcggacaggt gcattacgtg ttgagtaact
     1981 ggacggatga agaatccgcg ctcctgcccg acaggtgcga aaaagccggt gaaatcataa
     2041 aaagtttctg ccttgccggc gtacagcaca cgatgaatca gtataataat aaatgagttt
     2101 tgagttatgt gttcggagtt ctgagttctg agttcggatt acctgattca tctaaacacc
     2161 agttaaccaa ttaactaatc actcttaagt ccgcctggga gatccagggg gcttatacca
     2221 cccaatggaa gtacggatag acaaatggtt gtgggcagtg cggctgttca aaacacgaag
     2281 cctggctacc gaagcctgta aaaaagggaa aattctcatt cagcaggctg ccgtaaaacc
     2341 ttcgcgcaca gtaaaggtag gagacatcat tcaaattaag cgtcctccgg tggtgtactc
     2401 cttcaaagtg cttgccctgg ctcaaaacag gatgaatgca cggcttgtgc cggaacacat
     2461 gcaggatatc accacacccg accagctcga actgatcgaa ctcggaaaaa tagctgctaa
     2521 aacagcccgt gaacgcggta cgggacgtcc gaccaagaaa gaacggcggg atatggacga
     2581 ctttttcgaa ccgttttttg tggatgatga agattaaatc cagctttcgg ctcgttcccg
     2641 ttttcactat tcattatttt tttaaaaaat acatttttca ccaaaaacaa aacaacccaa
     2701 ttccatgtat attgctacag ctctgcgaaa aaaaaatatt atcgaatacc tgctttatat
     2761 gtggcagatt gaagatttga tccgggcatt cgggctggat atgagcagga taaacgaaaa
     2821 aattgtggaa ccttatcagc tttcggacaa tgacaaaaaa aggttgtatg agtggtatga
     2881 gagcctgaca gaaatgatgc gaaccgaaaa tgctcaggaa gcaggtcata tccaactgaa
     2941 taaaaatatc gtaatccagc tgaatgattt tcatgccgaa gtgcttcaat cgggcatgct
     3001 gcatgattac aacagccgtt ttaacaggct ccggcccttt ctctctcagt tgcgcagcaa
     3061 acaaaacgat ccggaaataa gtgatataga gctgtgtttc aactttcttt acggaatcat
     3121 gacactccgg atgcaaaaag cagaaattac tcccgaaaca cttcaggtga aggatgaaat
     3181 cggcaagttt atgggtttgc tgaatgaata ttatcagcaa tatgtgagcg gagaattaca
     3241 gcttcaggaa gaataattta aaccatttta ccgagattat tcgcttttca aaagttccat
     3301 tttcacccgg cctaccttca tcctggcttc aatgagcatc ggaagacgtt ttgagtcggt
     3361 cgaaatccag attgtagccg gataatcctg gttgaaaact ttccctttac ccacctgcgg
     3421 cttgaaagcg tagcaactta caagcatatt attcgccctg atttgctcga tgccgttgaa
     3481 caccactttg gacatgatca ctttatcaga agtaaaaaaa ggaatggtaa ccgaatctcc
     3541 ttttttcatt tttgatgctt cgagagagcg gacataatat atcatggaaa caaaatcgtg
     3601 tgtatttggc ggaatagtga tccgcttatg ccggtaagtg gccacatggt tattctgatc
     3661 aaaaaacacc gtatcggcac ttcggtattt gttttctctc actgcctgac ggtacatgag
     3721 cggagcgccc gagtttacat cgatataggt ttcgtactta ttttccacct gataaaacca
     3781 ctcgataaca ccggtggtgg caccgtaaag agtagccaga taagtatcat gatttcccgt
     3841 tcttacggtt gaggatttca cactcattgt agcttcgcca cctttcacat tgcctgtaag
     3901 gcccgaactg aatgcaattt tgtatttcag tttttcgccc ctgataatat tccattcggc
     3961 tccgaacgaa aaccggctaa tcaataacaa aacaaaaaga acagtatata atcttctgaa
     4021 tgtattcata taaaaatttg ttttgaatct gttgtcaaaa attattccaa aaagttaact
     4081 ttgtatttct tttaaacata tttacgagat taagcagcac acgcttcttc ataaaaaaac
     4141 tggctgacat atttacataa gctgaaagaa aatctgactg gatttattac ctgaatgtgt
     4201 tgagacactt ttcagcagat ggcatataaa ttgatgtgta aaaatataat ccaggaaata
     4261 aacgttctgt ttattacaaa gtgtattgtt cgtttgttta aatttataat cctccaaaat
     4321 aatcgttatg aaacggattc aaattattgt catattgtcg gtcgtgctgg ttgccactgc
     4381 tgctaccatt gcattggctc aaaaatccaa aacatcatcc atggaaacac gctggaaaaa
     4441 agtagaacgt tttgccgaac agaatttacc ggaatcggca cttaaagaag tggaagccat
     4501 tcttggccag gcccgaaagg aaaaaaaatc gccggaaatc attaagtcaa tggtgtataa
     4561 aatgcgtttc atcctggaca agaatccgga tgaaaccgaa aaactcatcg ctgagtttga
     4621 agattacact attgcaggta ccgatccggc cgaaaaagcc attatgtact ccatgacggc
     4681 cgaattatac ttaaaatact accagaatga acggtataaa attaatcaac gcaccgaact
     4741 tcagggcttc gtaccggagg atatgaagga atggaccaga aatatatttg aggataaaat
     4801 tgtcggacta gcctgcaaat cgctggaaaa acccgaaacg ctgcagaaaa tcccctcact
     4861 gcaattcgcc gcaatactcg agcagggaaa agacagccgc gatatgcagc caacgctgtt
     4921 tgatcttttg tcgtatcgga aaattgactt gttgatgaat tttaaggaaa atgactccga
     4981 taacgaattt ccggaagaca aagctgactt cacttcctca ggtcagaatg ttttagcttc
     5041 tgaaaatcag cttactattc attcaaaaat tctcgacacc tacaacggga ttattgacta
     5101 caacaaaaaa caaggcaaca ctcccgcaat cgtttatgcc gaact
//
LOCUS       DAORKP010000151         5078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3737_length_5078_cov_8.763886, whole genome shotgun sequence.
ACCESSION   DAORKP010000151 DAORKP010000000
VERSION     DAORKP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_5078_cov_8.763886"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLN75_08010"
     CDS             <1..1148
                     /locus_tag="PLN75_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRG04099.1"
                     /translation="ASLMTGLHTGHTHVRGNKEYPPEGQEPMGEMKTLGNLFQQAGYA
                     TGIYGKWGLGFPGSGSEPTKKGFDHFYGYNCQRQAHTFYPNWLWKNDEKTVLTGKEYS
                     QDLIHRQALTFIRSNANKPFFAYITYTLPHAGLEQPDDSILKIYSGKFPETPYSGNGN
                     YAPADEPRAQFAGMVTRMDAYVGEIRNELQKLGIEENTLLIFTSDNGPHIEGGADPAF
                     FANKQTLRDTKRSLYEGGIRVPTIMEWRGTISAGVKSEFPSAFWDFMPTFVHLTHQQK
                     TWKQKTDGISILPTLTGKSQQQPHKYLYWEFHEEGGRQAVRVGDWKLIKQKIKSGNPT
                     FELYNLKNDPFEKRDLSAENPERVRKLKKRMDKAHTPSEMFNFGIDK"
     gene            1150..3714
                     /locus_tag="PLN75_08015"
     CDS             1150..3714
                     /locus_tag="PLN75_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HRG04100.1"
                     /translation="MRQIIYAILMLIWPLIGVAQQMESISMNDGWQFSKSGKNQWKQA
                     TVPGSVQRDLIRLGELPDPYWGTNEKLVQWVEDKNWDFRKTFTIDSVQLTSDEALLCF
                     EGLDTYADVFLNGSKILHTENMFLGYEVSVKNLLKKGENNLFIRFFSPIETILPAHTT
                     SGFDYPADNDHRMPRLSVYTRKAPYHFGWDWGMRMVQTGIWRPVSLLFYKKAQIKDYW
                     VQQIDIQPEKAILNNHLEIHSLSEKNADATLTIDYDPLILNRKPLQIQQKITLKEGKN
                     IISIPLEIDNPKLWMPRDWGEQNLYHFTVKVFSNNELIDSKTVTTGLRRIELIRDSDV
                     NGRSFYFKVNGIPLFAKGANYIPGEIINTQQDKAYYDRLFENITAANMNMIRVWGGGF
                     YEDDYFYRLADEKGILVWQDFMFACTTYPHDGTFMDNVEKEAEYNIKRLRNNPSVALW
                     CGNNEVAEALKYWGWEKKYTPEIFNDFKVGYDKLFKQLLPEKVKELDPAKYYIHSSPD
                     TANWGRPESLKLGDAHYWGVWYGRQPFEILNERVPRFMSEFGFQAFPEMKTIRSFAPP
                     EEWSLESPTMLTHQKSTTGNDAIKEYMERYYHTPRNFEDFVYIGLVMQGRGMSHGMKA
                     HRRNRPYCMGTLYWQLNDSWPVVSWSGIDYYGNWKAMHYHVRDAFKPLTIDIYEENGK
                     IGIFTLSDKLQDENDLKLEIELKDFSGKNIRKIKKIITAKANDSKKVLEVKSSDFVTE
                     TERKNSFLLVRLSNKQGKLITQEIHYFDAPKNLNLPETTIQKTIKKEERKLTITFKSK
                     MLAKDVFVEIPIQGAKFSDNFFDLLPGEKKVITITSPELKANGNDAIKVKHLRETY"
     gene            3717..4463
                     /locus_tag="PLN75_08020"
     CDS             3717..4463
                     /locus_tag="PLN75_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRG04101.1"
                     /translation="MYKIEICANSVTSCLEAQRGGAYRVELCAAIPEGGTTPSYGEIF
                     LARELLDIKLNVIIRPRAGDFLYSEIEHQTMLRDIEMCRKLRVDGVVFGCLTSGGEID
                     MNRNRELIEAAAGMSTTFHRAFDMCRNPMESLEKIIELGFDRILTSGGKPKAEQGIDL
                     LKKLTEKAGHRIIIMPGSGITEDNIAKIARETGASEFHLSARLPVASAMEYRNPAVSM
                     GGKNIEIDEYTFPVTSAERVKRTIENLNKN"
     gene            4477..>5078
                     /locus_tag="PLN75_08025"
     CDS             4477..>5078
                     /locus_tag="PLN75_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRG04102.1"
                     /translation="MKVLKKIIFTAVAFLIIAGTASLNAAEANYQVIPLPNSIKPAKG
                     TGFSPNSKTTIVYPKGNVKMQKNAEFLSDYLSQMNVAKLTIATSGKSNAIILQTGLKS
                     ANPEAYTLTVGKSSIIIKGASEAGVFYGIQTLRKATPIGGKSVIYPAVIITDAPRFEY
                     RGMHLDVARHFFPVSFVKKYIDMIALHNMNKFHWHLTEDQG"
BASE COUNT         1677 a         1126 c         1111 g         1164 t
ORIGIN      
        1 gcgccagcct gatgacaggc cttcacacag ggcatacaca cgtacgcggc aacaaagaat
       61 accctcccga aggccaggaa cctatgggag aaatgaaaac gctgggaaat ctatttcagc
      121 aggcaggata cgccaccgga atttacggaa aatggggtct cggattccca ggatcgggct
      181 cagagccaac caaaaaagga tttgatcact tctacggata caattgccag cgacaggcgc
      241 acactttcta tccaaactgg ctttggaaaa atgatgaaaa aaccgttctt accggcaagg
      301 agtattctca ggatttaatt caccggcagg cacttacctt tatacgcagt aatgccaaca
      361 aaccgttttt tgcctatata acctacacat tacctcatgc aggactcgag caacccgacg
      421 acagtattct gaagatttat tccggaaaat tcccggaaac tccctacagt ggaaacggaa
      481 attatgcccc tgctgatgaa cctcgtgcac aatttgcagg aatggtcacc cgaatggatg
      541 cgtatgtggg cgaaatcaga aatgaacttc aaaaactggg aattgaggaa aatacactgc
      601 tcattttcac cagcgataac ggaccgcaca tagaaggcgg tgcagatccg gcgttttttg
      661 ccaacaaaca aacgctgcgc gacaccaaac gctcgcttta cgaaggtgga ataagagtac
      721 ccacaattat ggaatggcgt ggaactattt ctgccggagt aaaatctgaa ttcccgtctg
      781 ctttctggga ttttatgccc acttttgtcc accttaccca tcagcaaaaa acatggaagc
      841 aaaaaaccga tggaatatcc attcttccaa ctctgacagg gaaaagccag cagcaaccgc
      901 acaagtatct ctactgggaa tttcatgagg aaggtggccg ccaggcagta agagtaggcg
      961 actggaagct gataaaacag aaaataaaaa gcggaaatcc gacttttgaa ttgtataatc
     1021 tgaagaatga cccgtttgaa aaaagagatc tttcggcaga aaatccggaa agagtcagaa
     1081 agctgaaaaa acgaatggac aaagcacata ccccttcgga aatgttcaac tttggtattg
     1141 acaaatgaga tgagacaaat catatatgct attctgatgc tgatatggcc attaataggt
     1201 gtagctcaac aaatggaatc aatatcaatg aatgatggct ggcaattttc caaaagcgga
     1261 aagaaccaat ggaaacaagc cacagtgccc ggttcggttc aacgtgatct gatccgattg
     1321 ggcgaactgc ccgatcctta ctggggaaca aacgaaaaac tcgttcaatg ggtagaagat
     1381 aaaaactggg atttcagaaa aacgtttaca attgattcag tacaacttac atccgacgaa
     1441 gctttgcttt gtttcgaagg acttgatacg tatgcagatg tttttctgaa cggatcgaaa
     1501 atacttcata cagaaaatat gtttctgggc tatgaagttt cggtgaaaaa tctgctgaaa
     1561 aaaggcgaaa acaatctttt tatacgattt ttctcaccca tcgaaaccat cctgcctgct
     1621 cacactacaa gcggattcga ttatccggcc gacaacgacc accgaatgcc ccggctgagt
     1681 gtgtacaccc gcaaagcacc ctatcatttc ggctgggact ggggaatgag aatggttcaa
     1741 accgggatct ggcgaccagt ttcgctactt ttttacaaaa aagcacaaat aaaagactac
     1801 tgggtgcagc agattgacat tcagccggaa aaagcaattc tcaacaatca ccttgaaatt
     1861 cattcccttt ctgaaaaaaa tgcagatgcc acattgacaa ttgactatga tccattgatt
     1921 ttaaatcgaa aaccactaca aatccaacag aaaataacct taaaagaagg aaaaaacatt
     1981 atttccattc cgctggaaat tgacaatcca aagctctgga tgcccagaga ctggggtgaa
     2041 cagaatttgt atcattttac tgtaaaagtt ttttcaaaca acgaattgat tgatagcaag
     2101 acggttacaa ccggattgag aagaattgaa cttattcggg atagcgatgt taatggtcga
     2161 tctttctatt tcaaagtcaa cggaattccg ctttttgcca aaggagccaa ctacattccc
     2221 ggtgaaataa tcaacactca acaggataaa gcatactacg accgcctttt tgaaaatatt
     2281 acggctgcca atatgaatat gatccgtgta tggggaggtg gattttacga agacgactac
     2341 ttctaccggc tggccgacga aaaaggcata ctggtatggc aggattttat gtttgcctgc
     2401 actacttatc cgcacgacgg gacatttatg gataatgtag aaaaagaggc agaatacaac
     2461 atcaaacgtt tacgcaataa tccttcggta gcgctgtggt gtggcaataa cgaagtggct
     2521 gaagcgctga aatactgggg ctgggagaag aaatatacgc ccgaaatatt caacgatttc
     2581 aaagtgggct acgacaaact tttcaaacag ctactgcccg aaaaagtgaa agaacttgac
     2641 cccgcgaaat attacattca ctcctcgccc gacacagcca actggggccg ccccgaaagt
     2701 ctgaaactgg gcgatgccca ttactggggt gtgtggtacg gacgccaacc gtttgaaatt
     2761 ctcaacgaac gggttccccg ctttatgagt gaattcggat ttcaggcgtt ccccgagatg
     2821 aaaaccatcc gcagctttgc accgcccgaa gaatggagcc tcgaaagccc gaccatgctt
     2881 acccaccaga aaagcaccac cggcaacgac gccatcaaag aatacatgga gcggtactat
     2941 cacacaccac gtaattttga ggattttgtg tatatcgggc tggtgatgca gggacgcggc
     3001 atgagccacg ggatgaaagc gcaccgaaga aaccgtccgt actgcatggg tacgctttac
     3061 tggcaactga acgacagctg gccggtagtg tcgtggtcgg gcatcgacta ctacggcaac
     3121 tggaaagcga tgcactatca cgtacgggac gctttcaaac ctttaaccat agacatttac
     3181 gaagaaaacg ggaaaatcgg catcttcacc ttatccgaca aactgcagga tgaaaatgac
     3241 ctgaaacttg aaattgaact gaaggatttc agcggaaaaa acattcggaa aatcaaaaaa
     3301 atcatcacag ccaaagccaa tgattccaaa aaagtattgg aagtgaaaag cagtgatttc
     3361 gtcacagaaa cagagcgaaa aaacagtttt ttacttgtta gactttcgaa taaacaagga
     3421 aagctgataa cacaggaaat tcattatttc gacgcaccga aaaacctgaa tttacctgaa
     3481 acaactattc agaaaacaat caaaaaagag gaaagaaaac tcaccatcac gttcaaaagc
     3541 aagatgttag caaaagatgt atttgtggaa atccccatcc agggagcaaa attctcggat
     3601 aatttctttg atttattgcc gggcgaaaag aaagtaataa ctatcacttc gccggagctg
     3661 aaagccaatg gaaatgatgc aattaaggta aaacatttga gagagaccta ttaataatgt
     3721 ataaaataga aatttgcgcc aattccgtta ccagttgcct cgaagctcag cgtggcggag
     3781 cgtatcgggt ggagctgtgt gctgcaattc ccgaaggggg aacaacccct tcttacggcg
     3841 aaatttttct ggctcgcgaa ctgttggaca tcaaactgaa cgtgattatt cgtccgcgcg
     3901 ccggtgattt tctttattcc gaaatcgaac accaaaccat gctacgggat attgagatgt
     3961 gccgaaaact gagagttgac ggagttgtat ttggctgcct cacatcaggt ggcgaaattg
     4021 acatgaacag aaaccgggaa ctgattgaag cagccgcagg aatgagcaca acttttcacc
     4081 gcgcttttga tatgtgccgg aatcccatgg aaagccttga aaaaataatc gaacttggtt
     4141 tcgacagaat tctgacttca ggtggaaaac caaaagccga acagggcatt gatttactga
     4201 aaaaattaac agaaaaagcc ggtcaccgta tcatcatcat gcccggaagc ggcattacgg
     4261 aagacaacat tgcaaaaatt gcacgcgaaa caggcgcctc tgagtttcat ctttctgcca
     4321 gattgccggt ggcaagcgca atggaataca gaaatccggc agtgtcgatg ggaggaaaaa
     4381 acatcgaaat cgacgagtac acttttcctg taaccagcgc cgaacgggta aaacgaacaa
     4441 ttgaaaatct aaataaaaac taaaaaaatc aacaacatga aagtattgaa aaaaatcatt
     4501 ttcaccgctg tggcttttct gataatagca ggtactgcaa gcttaaacgc agctgaagct
     4561 aattatcagg tcattccatt gccaaattcc attaaaccgg cgaaaggcac aggattttca
     4621 ccaaattcaa aaacaacaat cgtttatccg aaagggaatg ttaaaatgca gaaaaacgcc
     4681 gaatttctgt cggattacct ctcacagatg aacgtggcaa aattaacaat tgccacatcg
     4741 ggcaaatcaa atgcaattat tctgcaaacc ggattaaaat ctgccaatcc cgaagcttac
     4801 acgctgactg tcggaaaatc gtccattatc atcaaagggg cgagcgaagc cggcgtgttt
     4861 tacggtatcc aaaccctgcg caaagccact cccattggcg ggaaatctgt aatttatccg
     4921 gctgtaatca tcacagatgc accgcgtttt gaatatcgcg gcatgcacct ggacgtagca
     4981 cggcatttct tcccggtgtc atttgtaaaa aaatacatcg atatgattgc tttgcacaac
     5041 atgaacaaat ttcactggca cctgaccgaa gatcaggg
//
LOCUS       DAORKP010000152         5078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3738_length_5078_cov_7.478001, whole genome shotgun sequence.
ACCESSION   DAORKP010000152 DAORKP010000000
VERSION     DAORKP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_5078_cov_7.478001"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1995)
                     /gene="argS"
                     /locus_tag="PLN75_08030"
     CDS             complement(202..1995)
                     /gene="argS"
                     /locus_tag="PLN75_08030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRG04103.1"
                     /translation="MNLETQLTQGVITAIQKLYNQIVDGGTVQFQKTKKEFEGDITLV
                     VFPFVKIARKSPEQTAAEIAEELKKQYPMISSYNIIKGFLNLNIDSSFWLEELDEMHN
                     DNRFGTVATTEDAPLMMIEYSSPNTNKPLHLGHIRNNLLGYSIAEIQKANGWNVVKTN
                     IVNDRGIHICKSMLAWKLFGNGETPESTGMKGDHLVGKYYVEFDKAYKAEIRELMEKG
                     LSEEDAKKEAPLIRQAQEMLLKWEQGDEEVRSLWHTMNGWVYAGFDETYRKLGVDFDK
                     IYYESDTYTEGKSKVEEGVAKGVFYQREDNSVWADLTADGLDEKLLLRADGTSVYMTQ
                     DIGTAKIRYEEYPINKMVYVVGNEQNYHFQVLSILLDKLGFAWGKDLVHFSYGMVELP
                     EGKMKSREGTVVDADDLIEEMIGTARETSQELGKLDGISENEALHVARMVGLGALKYF
                     ILKVDPKKNMTFNPKESIDFNGNTGPFIQYTHARIKSVLRKADEQGISYKSLTDKNIP
                     LSEKENYLVQTLTEFPQIVKEAGDEFSPALIANYIYELVKEYNQFYHDFSILREENEH
                     LKVFRLVLSETIAHVIKTGMGLLGIEVPERM"
     gene            complement(2173..2445)
                     /locus_tag="PLN75_08035"
     CDS             complement(2173..2445)
                     /locus_tag="PLN75_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRG04104.1"
                     /translation="MNKGELISAIAQASGLTKVASKKALDATIKSISDALAKGDKVTL
                     VGFGTFSVAERAARKGVNPSTKQPIRIKAKKVAKFKAGSELADVVK"
     gene            2686..3471
                     /locus_tag="PLN75_08040"
     CDS             2686..3471
                     /locus_tag="PLN75_08040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRG04105.1"
                     /translation="MNRSLPPVVKNILIINVILYLATLMLPTILRRWGIDMRLEDVLG
                     MHYVGSGKFNLLQVVSYMFMHGSVTHILFNMFAVYMFGPIFEQVWGSRKFLFYYLVTG
                     IGAGLVQQLFWYVELGDVFRAMNAAVSSGSAEALAPYSAELSHYFRFSGDIAQMPVTQ
                     VLQMKKELADALLTIGASGAVFGILLAFGWLFPEQKLFLIFFPVPIKARWFVAGYAVA
                     ELFLGVANFAGDSVAHFAHLGGMLFGVLLILYWKKKGKLYSSH"
     gene            3487..4365
                     /locus_tag="PLN75_08045"
     CDS             3487..4365
                     /locus_tag="PLN75_08045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRG04106.1"
                     /translation="MNLIDNLKLTYKNGSTVIKLIYVNVAIFLFVSILSVVFRLFNLT
                     SIDYTTWFSVPSNPSQLLTHLWTPVTYMFYHEQFFHIFFNMLVLYWFGKIFTMYYSEK
                     QLLSLYILGGLTGAAVYVVAYNVFPYYEAASYYSVLMGASGSILAILVASAVRAPNME
                     MQLLLIGRIKLMWIAVITVLISFFGLTSENGGGEMAHLGGALAGYLFVVFEKKGTDIT
                     RFINKIIDFFVNLFRPRPRKKPTVYHAQKMSPEQYNQKKAQDEVEINRILDKIKTSGY
                     ESLSADEKRKLFEQKR"
     gene            4331..>5078
                     /locus_tag="PLN75_08050"
     CDS             4331..>5078
                     /locus_tag="PLN75_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04107.1"
                     /translation="MKKGSFLNKKDKTMTKFLRGIMILINIFVVLALVLVKIGSVVSP
                     NTALLPAYASLGLLPIIILNIAFIVFWIMLRKWWSLLSLLALIIFSGIIKSAVPVNLT
                     RTEADSTRTKITLLSYNTMNTGLMKKHRKDDPNPVVQYILDSDADIVCLQEFAVSENK
                     RQFQSDDFERIFSKYPHKHVSFQLDKWRMNIGLATLSKYPIIEKRNVDYESVFNLSIY
                     TDVVIGDDTIRILNNHLESNRITSMDMKQTA"
BASE COUNT         1245 a         1089 c         1142 g         1602 t
ORIGIN      
        1 aaaatagttt taaaaatatc agtttaaatt aatgtagttt atcgggggct tacgacaaaa
       61 cgaccgatac aacatttcat taccgggaaa aagataagtc cgtttgtcga tgaaaaatac
      121 acccgaaaaa accggatgaa aaatccggaa tgacacatac atatcaacat atttggaaat
      181 ccgggttgta aatacaattc cttacattct ttccggcact tctattccca gtaatcccat
      241 gccggttttg atgacgtggg cgatggtttc cgagaggacg aggcggaaca ctttcaggtg
      301 ttcgttttct tcgcggagga tggagaaatc gtggtaaaac tggttgtatt cctttaccag
      361 ctcgtagatg tagttggcta tcagcgcggg gctgaactcg tcgccggctt cttttacaat
      421 ctgaggaaat tcagttagcg tttgtaccag gtaattttct ttttccgaaa gcggtatatt
      481 cttgtcggtc aatgatttgt agctgattcc ctgttcgtcg gctttgcgga gcacggattt
      541 gatgcgggca tgtgtatatt gaataaacgg acctgtgttg ccgttgaaat cgatggattc
      601 tttcggattg aaggtcatgt ttttcttcgg gtccactttc agaatgaagt atttcagtgc
      661 gccaagtccc accatgcggg caacatgaag agcttcattt tcagaaatac cgtctaattt
      721 tcccagttcc tgggaggttt cacgggcagt gcctatcatt tcttctatca gatcgtcggc
      781 atccaccacc gttccttccc ggcttttcat ttttccttcg ggaagttcca ccatgccgta
      841 ggagaaatgc accagatctt ttccccaggc aaaaccgagt ttgtcgagca gaatggacag
      901 tacctggaag tggtaattct gttcatttcc aaccacatac accattttat tgatcgggta
      961 ctcttcgtag cggattttag ctgttccgat gtcctgcgtc atgtaaaccg aagtgccgtc
     1021 ggcgcggagg agaagtttct cgtccagccc gtcggcggta aggtctgccc acacggaatt
     1081 atcctcacgc tggtaaaaca ctccttttgc caccccttct tccaccttgg attttccttc
     1141 ggtgtaggta tcggattcgt agtatatttt atcaaaatcg actcccagtt tacggtatgt
     1201 ctcgtcgaaa ccggcgtaca cccatccgtt catcgtgtgc cacagcgagc gcacctcttc
     1261 atcgccctgt tcccatttca gcagcatttc ctgtgcctgg cggataagcg gagcctcttt
     1321 tttggcatct tcctcgctca ggcctttttc catcagttcc ctgatttcgg ctttgtaggc
     1381 cttatcgaat tccacgtaat atttccccac caggtggtcg cctttcatgc cggtgctttc
     1441 cggtgtttcg ccgttaccga agagtttcca ggcaagcatg gatttgcaga tatgaatgcc
     1501 gcgatcgttt acaatgttgg ttttcaccac gttccacccg ttggcctttt gaatttcggc
     1561 aatgctgtag ccgagcaggt tgttgcggat gtgaccgagg tgaaggggtt tgtttgtgtt
     1621 tggcgatgag tattctatca tcatcagcgg agcatcttct gtcgtagcga cggtgccaaa
     1681 ccggttgtcg ttatgcattt cgtcaagttc ctcaagccag aatgaactgt cgatattcag
     1741 attcagaaaa cccttgatga tgttgtacga actgatcatc ggatactgtt ttttaagttc
     1801 ttcagctatc tctgcggcag tctgttcggg cgatttgcgg gcaattttta caaaagggaa
     1861 aaccaccagc gtgatatctc cttcgaattc cttttttgtc ttttgaaatt gcactgtacc
     1921 gccatcaaca atctgattgt atagtttctg tatggcagta attacacctt gggtaagttg
     1981 agtttctaaa ttcatctgtc ggaagaaaaa ataattgttt gaaagcgcaa agatacggat
     2041 ttctgtgaaa ttgtgcatta cacgtttaga gaatggttga ttttttaaag aggtaacagt
     2101 tttttcataa aacaaacagg gtgtccgcta tgattgcgaa cgccctgttt gttaatgagt
     2161 tatgagaatt ttttatttaa ctacatcagc cagttcagat cctgctttga atttagctac
     2221 ttttttagct ttaattctga ttggttgttt ggtggaaggg ttcacgcctt ttcgggcggc
     2281 acgttcagct acagaaaatg ttccaaatcc gacaagagta actttgtcgc cttttgcaag
     2341 ggcatcgctg atgctcttga tggtggcatc cagcgctttt ttggaggcaa cttttgtcag
     2401 gccggaagct tgagcaatag cactgattaa ttctcctttg ttcatagttt tttagttttt
     2461 tagtttaaga cattaaaagc caaatacttc ggtgctgcaa atataattaa tacgaattaa
     2521 taaacaaatt ttttgtttag aaaaatgtat ttttttccta aagaagttca atatcctgtc
     2581 caacaacata gttttgaagt ttttttcgta attttgcagc tcatttgaaa gcaggcatgt
     2641 tcttttcaca gcctgaattt ctgagaaaaa acaaaacgat tgtttatgaa cagatccttg
     2701 cctccggtag ttaaaaatat tctgattatc aatgtcattc tatacctggc tactttgatg
     2761 cttcccacca ttctccgaag gtggggaata gatatgcgcc tggaagatgt cctcgggatg
     2821 cattatgtcg gatccggaaa attcaacctg ctgcaggtcg tttcgtacat gtttatgcac
     2881 ggctcggtta cgcatatact tttcaatatg tttgccgtgt atatgttcgg acccattttc
     2941 gagcaggtgt ggggttcgcg gaagtttctt ttttattatc tcgtaaccgg catcggagcc
     3001 ggacttgtac aacaactctt ctggtacgta gagctgggcg atgtattcag ggcgatgaac
     3061 gctgctgttt cgtctggttc ggctgaggca ttggctcctt attcggccga actttcacat
     3121 tacttccgtt tttcgggtga tatagctcag atgcctgtca cacaagtgct tcagatgaag
     3181 aaggaactgg cagatgcgct gctcacaatc ggtgcttcgg gtgccgtttt cggcattttg
     3241 cttgctttcg gatggctgtt tcccgagcag aaactttttc tgattttctt tccggtgcct
     3301 atcaaagccc gctggtttgt ggccggatat gcggtggctg aattattcct cggagtagcc
     3361 aattttgcag gcgacagtgt agctcatttt gcccaccttg gcggaatgct tttcggggtg
     3421 ctgctcattc tttactggaa aaagaaaggt aaactttatt cttcacacta aaaaacaatc
     3481 acgtaaatga atctcattga taatctgaaa cttacttata agaatggcag cacggtgatc
     3541 aaactcattt atgtaaatgt ggccattttt cttttcgtaa gtatcctctc ggtcgttttc
     3601 aggttgttta atcttacttc cattgattac accacgtggt tttccgtacc ttccaatccc
     3661 tcacagctgc ttacccatct gtggactccc gtaacgtaca tgttttacca cgaacagttt
     3721 tttcacatct ttttcaatat gctggtgttg tactggtttg gtaagatttt caccatgtat
     3781 tactctgaaa aacagctgct ttcgttgtat attctcggcg gattaacggg cgccgcggtg
     3841 tatgtggtgg cttacaatgt ttttccgtat tacgaagcag cttcgtacta cagcgtgttg
     3901 atgggagctt caggttctat tctggctatc ctggtggcat cggcagtacg agcaccgaac
     3961 atggaaatgc agttgctgct cataggtaga atcaaactga tgtggattgc cgtgattacg
     4021 gttctgatta gttttttcgg gttgacttcc gaaaacggag gcggcgaaat ggcccatctg
     4081 ggcggtgcgc tggccggtta tctttttgtg gtttttgaga aaaaaggtac agatattacc
     4141 cggtttatta acaaaataat cgattttttc gtaaatttgt tccgtccgcg cccccgaaag
     4201 aaaccgactg tttatcatgc gcaaaaaatg tcgcccgaac agtacaatca gaagaaagca
     4261 caggacgagg tggagattaa ccgcattctg gataaaatta agacatcggg ctacgaaagc
     4321 ctttcagccg atgaaaaaag gaagcttttt gaacaaaaaa gataaaacaa tgaccaaatt
     4381 cctgcgtgga ataatgatat tgattaatat attcgtcgtt ttggcgctgg tattggtcaa
     4441 aatcggttca gtagtcagtc cgaatactgc acttttgccc gcttatgcat ccctggggct
     4501 gctcccaatc attattctga acattgcctt tattgttttc tggattatgc tgcgcaaatg
     4561 gtggtcgctg ctctcgttgc ttgcccttat cattttttcg ggaatcatta aaagtgctgt
     4621 tcctgtaaat cttacccgga cagaagctga ttctacccga acaaaaatta cgctcttgtc
     4681 gtacaacacg atgaataccg ggctgatgaa gaagcaccgg aaagatgacc ctaatcccgt
     4741 agttcagtac atcctggatt ccgatgccga tattgtatgt ctccaggaat ttgctgtgag
     4801 tgaaaataaa aggcagtttc agtcggacga tttcgaacgg attttcagca agtacccgca
     4861 caagcacgtg agttttcagc tggataagtg gagaatgaac atcggtctgg ccaccctttc
     4921 gaaatatccg attatcgaaa aaagaaatgt ggactacgaa tctgtattca atctgtccat
     4981 ttataccgat gtggtgatag gggacgatac cattcgtatt cttaataacc acctggaatc
     5041 caacaggata acttcgatgg atatgaagca gaccgccg
//
LOCUS       DAORKP010000153         5059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3769_length_5059_cov_8.865108, whole genome shotgun sequence.
ACCESSION   DAORKP010000153 DAORKP010000000
VERSION     DAORKP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_5059_cov_8.865108"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..>1099
                     /locus_tag="PLN75_08055"
     CDS             1..>1099
                     /locus_tag="PLN75_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRG04108.1"
                     /translation="MPSGGGGALISGIQQIGIGVENLYEAWKYYIDVFGMDIRILEDN
                     KVAELMLPYTGGVPQRRHACIAVNMQGGGGFEVWQYAERKPKKLDFELNMGDLGVLVC
                     KIKSKDVAKSFEEFSRNPKVNIVTRLSRNLDGMQTFYVKDPYGNLFQIVHDDYIFKNE
                     GRLTGGAVGATIGVTDIEKAMTIYRDILGYDTIISDETGVFPDFDGLPSGNQKFHRRL
                     LTHSKPRKGAFSELFGPSYIELVQALERTPRKLYEGRFWGDPGFIQVCFDIRNMEELG
                     KLCTEKGHPFTVDTTQKFKEGSSFDMGDAAGQFAYIEDPDGTLIEFVEAHKVPIVKKL
                     GIAINLKKRNPEKALPKFILKALSVMRVSSRQ"
     assembly_gap    1100..1199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1393..2709
                     /locus_tag="PLN75_08060"
     CDS             1393..2709
                     /locus_tag="PLN75_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRG04109.1"
                     /translation="MKKENLPVRMLMLLTGIFVIAFGVALSIRSNLGTTPISSVPYVY
                     NIVFPAVTVGTFTILLNLLFVVLQIVLLRKNFKPYLLLQVPVVFIFGWFIDFSLYLLG
                     GAVPGNYLVQWVFCIASCVIIAFGVFLQVKANVVMLPGDGLVIALADTLRKEFGLTKI
                     LFDSFLVLVSLITVLIIREGFHGVREGTVASALLVGFIVQFYQKHIRFVDKLTDTAVA
                     AKEYVPEPYMTTDNYVITISRQYGSAGHAVGELIAQKLGISFYDSKLIDLTAEASGFT
                     PEYVKAHEQKLSNGLLYQLYKQNYAYVNEAIPPQDMLFMVQTKVIRDIAARESCVIVG
                     RCADFILKGHPHCFNVFVHADDAFRLNRVITDYGADPEEAEKEMEKKDRERMNYNKHY
                     TKREWANLKDFDMTVESSMFGIEITAAMIIDARRKAIFTASDSSLS"
     gene            3129..>5059
                     /gene="gyrB"
                     /locus_tag="PLN75_08065"
     CDS             3129..>5059
                     /gene="gyrB"
                     /locus_tag="PLN75_08065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRG04110.1"
                     /translation="MTEEQINEAGEQYGASSIQVLEGLEAVRKRPAMYIGDVGIKGLH
                     HLVYEVVDNSIDEALAGYCDNITVTINEDNSITVTDNGRGIPVDIHEKEGRSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSFMRVEVARNKKMYVQEYSCGHPQFD
                     VKLIGDTADQGTKVTFLPDTSIFIESVYKYEVLANRLRELAYLNAGITLTLTDKREKD
                     ENGDFISETFFSREGLKEFVAYIDESREDLIDNVIHISTEKNGTPVEIAMLYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDNKLLEKLEKAKIEISGDDFREGL
                     TAVISIKVAEPQFEGQTKTKLGNTEVMGSVEMAVSEALGNYLEENPKDARTIVDKVVL
                     AATARHAARKARELVQRKSPLSGGGLPGKLADCSSKDPARCELFLVEGDSAGGTAKQG
                     RSREFQAILPLRGKILNVEKAMQHKVLESEEIKNIYTALGVFIGTEEDSKALNLSKLR
                     YHKVIIMTDADVDGAHIATLLMTFFFRHMKEVIEQGYLYIATPPLYLCKKGKVEEYCW
                     TEQQRLAFIDKYGGGNESAIHVQRYKGLGEMNESQLWDTTMNPENRTLRQVTIENAAT
                     ADHVFSMLMGDDVAPRRQF"
BASE COUNT         1465 a         1072 c         1176 g         1246 t
ORIGIN      
        1 atcccttcgg ggggtggagg ggctctcatc tccggcatac agcaaattgg catcggtgta
       61 gaaaatctct acgaagcctg gaaatactac atcgatgtgt tcggaatgga tatccgaatt
      121 ctggaagaca acaaagtggc ggaactgatg ctcccttaca cgggcggcgt tccacaacgg
      181 cgacatgcgt gcattgccgt gaatatgcag ggtggtggcg gctttgaagt gtggcagtac
      241 gccgaacgga aaccaaaaaa actcgatttt gaattgaata tgggcgattt aggcgtgctg
      301 gtttgcaaaa taaaaagtaa agatgtcgca aaatctttcg aagagttttc ccgaaacccg
      361 aaagtgaata ttgttacccg attgagccga aatctggacg gaatgcaaac tttctacgtg
      421 aaagatccgt acggaaatct gtttcaaatt gtgcacgacg attatatttt caagaatgaa
      481 ggtcggctca cgggcggtgc tgtgggtgca actatcggtg tgaccgatat tgaaaaagca
      541 atgacgattt atcgggatat tctgggttac gacaccatta tttccgatga aacaggtgtt
      601 tttcccgatt ttgacggatt gccatcgggc aatcagaaat tccatcgtcg attgcttacg
      661 cattccaaac cacgtaaagg agcattcagc gaattgttcg gaccatcgta catcgaactg
      721 gtacaggcac tggagcgtac accccgcaaa ctctacgaag gacgattctg gggcgatccg
      781 ggctttattc aggtttgctt tgatatccgg aatatggagg aactggggaa actctgtact
      841 gaaaaaggac atccgtttac cgtggatacc actcagaagt ttaaagaagg tagctccttt
      901 gacatgggcg atgcggcagg acagtttgcc tacatagaag atccggatgg cacgctgata
      961 gaatttgtgg aggcacacaa agttccgatt gttaagaaac ttggcattgc aatcaacctg
     1021 aaaaaacgta atcccgaaaa ggcattgccg aaatttattt tgaaagcgtt gagcgtgatg
     1081 cgggtcagta gccgtcaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1201 tctttaagta gcagctaacg aaatagttag cagctactgc agtagccgtc aactgaaagt
     1261 tgactgctac ttggagagaa gaaaagaatt ttttttaagt aacagctaac aaaatagtta
     1321 gcagctactg cagtgactgc tactttaaga aaaagtttta ttagttttgc attctaagaa
     1381 ttttgaataa aaatgaaaaa agaaaatctt ccggtacgta tgctgatgct gttaacaggc
     1441 atttttgtca tagcattcgg ggtggcgctc tccattcggt caaatctggg aactacgccc
     1501 atttcatctg tgccctacgt ttacaacatt gttttcccgg ctgttactgt cggaactttc
     1561 actatactgc tcaatttgtt gtttgttgtt ttgcagattg ttttactgcg taagaatttc
     1621 aaaccctatc ttttattgca ggtgccggtg gtatttatat ttgggtggtt tattgatttc
     1681 agtctgtatt tactgggcgg agcagtaccc ggtaactatc ttgtgcaatg ggtattctgt
     1741 attgcaagct gtgtaatcat cgcattcggt gtgtttctgc aggtgaaagc caatgtggtg
     1801 atgctgcccg gcgacgggct ggtaattgca cttgccgata ccctgaggaa agaattcgga
     1861 ctcactaaaa ttctttttga ttcattcctt gtactcgttt cactcattac agtactgatt
     1921 atacgcgagg gctttcacgg agtgcgcgaa ggaacggtag cttcggcgct ccttgtcgga
     1981 tttattgtcc agttttacca aaagcacatc cgcttcgtgg ataagcttac cgacaccgca
     2041 gttgctgcca aagaatatgt tcctgaaccc tacatgacca ccgacaatta cgtgataacc
     2101 atttcgaggc aatacggtag cgccggacat gctgtgggtg aacttatagc tcagaaactt
     2161 ggaatttcat tctacgacag caaactcatt gacctcactg ccgaagcaag tggtttcact
     2221 cccgaatatg tgaaagcaca cgagcagaaa ctctccaacg gacttcttta tcagctttac
     2281 aaacagaatt atgcttatgt aaacgaggca attcctcctc aggacatgct tttcatggtt
     2341 cagaccaaag tgatacgcga cattgctgcc cgcgaatcgt gtgtgatagt aggccggtgt
     2401 gccgatttta ttcttaaggg acatccgcac tgtttcaacg tatttgtaca tgcagatgat
     2461 gcattcaggc tcaaccgcgt gataaccgac tatggagccg accccgaaga agccgaaaag
     2521 gaaatggaaa agaaagacag ggaacggatg aactacaaca agcactacac caaacgtgaa
     2581 tgggcaaacc tcaaagactt cgatatgacg gtggaaagtt caatgtttgg catcgaaata
     2641 accgccgcca tgattatcga cgcccgcaga aaggccattt tcacggcttc tgattcctcc
     2701 ctgtcataaa taatccaacc ttttccgcaa gaacaaatgg tttttgcctg cttctaactt
     2761 atatgtcatt aaacattttt atagttttca cgccttttaa tgcatgggtt tttataaagt
     2821 agattaaaga gatttagcca acatttatca aactttggct taagccaatc tcttaaaacc
     2881 attaaattca cgcctaaaaa ggcgtggcaa tggattttgg tcatcatgcg gataatcaaa
     2941 taatacttta cgaaatgcat tgaaaaaata atgataaatc tgtgaaatat ttagctaaat
     3001 ttggcttgtt cttctttttc ggaatgggat ttttttcgta tatttgtatg ttttttgggt
     3061 tggtaaatcc atgtgaaaac agaacaacag aatattggat aatattctga aaattaaaga
     3121 gataaaaaat gacagaagaa caaattaacg aagctggaga acaatacgga gccagcagta
     3181 ttcaggtact cgagggattg gaagcagtaa gaaaacgccc cgccatgtac ataggcgatg
     3241 taggtataaa aggactccat cacctggttt acgaagtggt ggataactcc atcgatgaag
     3301 ccctcgccgg ttattgcgac aatataacag taacgatcaa cgaagataac tccatcacag
     3361 tgactgacaa cggacgcgga atacctgttg acattcacga gaaagaagga cgatcggcgc
     3421 tcgaagtggt gatgactgtg ctccatgccg gaggtaaatt cgacaaagga agttacaaag
     3481 tttcaggagg acttcacggg gtaggcgttt cgtgtgtgaa tgcactttcg tcgttcatgc
     3541 gggtggaagt ggcccgtaac aaaaaaatgt acgttcagga gtactcctgc gggcaccccc
     3601 agtttgatgt aaaacttatc ggagatactg cagatcaagg cactaaagta actttcctgc
     3661 ccgatacttc catatttatc gagtcggtgt ataaatacga agtgctggcc aaccgtctgc
     3721 gcgaactggc ttacctcaac gccggaatca ccctcacgct caccgataaa cgcgagaaag
     3781 atgagaatgg agatttcata agtgaaacat tcttctcccg cgaaggattg aaagaatttg
     3841 ttgcctacat cgacgaaagc cgtgaggacc tgattgacaa cgtcatccac atttcgaccg
     3901 agaaaaacgg cacaccggta gaaattgcca tgctttacaa tacctcgtac aatgaaaata
     3961 ttcactcgta tgtgaataat atcaacacca ttgagggagg aactcacctg gccggtttcc
     4021 gacgcggact tacacgtacg ctgaaaaaat acgccgaaga caacaaactg ctcgaaaagc
     4081 tcgaaaaagc aaaaatagaa atcagcggcg atgactttcg cgaagggctt acagccgtta
     4141 tctccatcaa ggtggccgaa ccgcagttcg aaggacagac caaaaccaaa ctcggaaata
     4201 ccgaggtaat gggatcggtg gaaatggctg tgagtgaagc actcggcaat tacctggaag
     4261 aaaatccgaa agatgcacgc accatagtag ataaagtggt actggctgcc acagcacgcc
     4321 atgctgcccg caaagcccgc gaactcgttc agcgcaaatc gcccctttcg ggtggcggac
     4381 tgcccggaaa actggccgac tgctccagca aagatccagc cagatgcgaa ttatttctcg
     4441 tcgaaggaga ctcggccggt ggtactgcca aacagggacg cagccgcgag tttcaggcaa
     4501 ttttaccttt gaggggaaaa attctgaacg tggaaaaagc catgcagcac aaagtgctcg
     4561 aaagcgaaga aattaaaaat atctatacgg cgctcggcgt gtttatcggt accgaagagg
     4621 acagcaaagc gcttaatctg agcaaactgc gctatcataa ggtaatcatc atgaccgatg
     4681 ccgatgtgga cggagctcac attgccacgc tgctcatgac attcttcttc cgacacatga
     4741 aagaggtaat agagcaggga tatctctata ttgccacacc tccgctatat ctgtgtaaaa
     4801 aaggaaaagt ggaggaatac tgctggaccg aacaacaacg ccttgcattt atcgataaat
     4861 acggaggcgg aaacgaatcg gccatccatg tgcagcgcta caaagggttg ggagaaatga
     4921 acgaaagtca gttgtgggac accaccatga atcctgagaa ccgcacgctg cgtcaggtaa
     4981 ccatcgaaaa cgctgctaca gccgaccacg ttttctccat gctgatgggc gatgatgtgg
     5041 caccgcgccg ccagtttat
//
LOCUS       DAORKP010000154         4916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_3988_length_4916_cov_9.182267, whole genome shotgun sequence.
ACCESSION   DAORKP010000154 DAORKP010000000
VERSION     DAORKP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_4916_cov_9.182267"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLN75_08070"
     CDS             complement(<1..244)
                     /locus_tag="PLN75_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04111.1"
                     /translation="MKLNQILLILAISALSLIFSACEKQDAYMDWKVMNNDWLEANKN
                     DKNSFPFNWTIDVLKTPLVNAEFQTTESGIKYKILRL"
     gene            complement(301..1194)
                     /locus_tag="PLN75_08075"
     CDS             complement(301..1194)
                     /locus_tag="PLN75_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04112.1"
                     /translation="MDKKKLTILIVVLTLVIAALVAGLIIFMNKSNKQSQQIAQTEQF
                     MEEEKQQMVTEVDALAGEMDGYTMYVHNDSLLREFDLQKQKIKDLQAELKSTKATDAK
                     RIAELKAEIATLRTILAHYVQQIDSLNSLNKRLTNENLEVKQRYQNVSETAEMLAKEK
                     ESLSEVVTRAAVLELYNFSFNPMDERNRKTERSSKMTNLKFNFTIGKNITAEPGPKTV
                     YLRLSRPDGEVMQKGNSVFAYENKNIEYSLKKEFDYTGEAHSDAVYWKVEEILQIGTY
                     QADFFVDGNRIGSYSFRIEKK"
     gene            complement(1340..2641)
                     /gene="murF"
                     /locus_tag="PLN75_08080"
     CDS             complement(1340..2641)
                     /gene="murF"
                     /locus_tag="PLN75_08080"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRG04113.1"
                     /translation="MRLYDIFLKHPLICTDSRNCPPDSIFFALKGDNFDANGFAAGAL
                     EKGCAYAVIDNPQYAVDERFIVVNDVLESLQELARFHRQKLGTKIIGITGTNGKTTTK
                     ELIASVLMEKFNTHFTRGNFNNHIGVPLTLLQLKPEHDIAVVEMGANHPGEIKILAEI
                     ALPDYGIITNVGKAHLEGFGSFEGVMNTKAELYDFILKKSGKIFLNTGNEYLKKMAEK
                     SGFRDANSILAYKTDSEINGNQATGTITSCSPLLRMQCVTSEGDFDVKTQLIGTYNAE
                     NVLAAVAIGNYLEVSNDQIKAGLENYQPRNNRSQFTETDKNKLIIDAYNANPTSLQAA
                     ILNFAQMEAPHKTLIIGDMLELGSQSAEEHQKVVDLVNESGFTDVFLVGPNFNATRNN
                     FSNFADVDLLKEYLQKNPLINRFILIKGSHGIHLEKVISEL"
     gene            2773..3048
                     /locus_tag="PLN75_08085"
     CDS             2773..3048
                     /locus_tag="PLN75_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04114.1"
                     /translation="METEKDKKDKVIQVVKDKFSKLPLGFIIGAAIGGGLYLYATRSK
                     GTKSVNAITNMAKLAAPYVIIAMLDKLVDTYETSRNREIEEAEAEVN"
     gene            complement(3139..>4916)
                     /locus_tag="PLN75_08090"
     CDS             complement(3139..>4916)
                     /locus_tag="PLN75_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRG04115.1"
                     /translation="IALHNMNKFHWHLTEDQGWRIEIKKYPKLTEVGAWRSETVIGHN
                     TGKFDGKPHGGFFTQDEIRDIVKYAQDRYVTVIPEIDLPGHMLAALASYPELGCTGGP
                     YEVEKSWGVFDDVLCAGREKTYTFLEDVLTEVCELFPSKYIHIGGDESPKVRWEKCPD
                     CQAKIKELGLKDDKKHKKEFYLQSYVMERMEKFLNGKGRRIIGWDEILEGTLAPNATV
                     MSWRGEAGGIEAARMGHDVIMTPNSYLYFDYYQTTDTKNEPDAIGGYVPVELVYSYEP
                     MSKELTPAEQKLVLGAQANIWTEYIPTTQQVEYMAMPRMAALADVQWTQPAKKDYADF
                     LRRIPRLLSLYDLLDYNYATHLYDVKADLKPNTGKGTLDVILSTLDNAPIYYTLDGTE
                     PSANSIRYTETISLNKDAVISAKAIRANGKNSRTFSEKVKLSKSSLKPITLLTTPDRN
                     YTYTGAGLLNDGLTGDTNYKTGRWMGFQGTDLIAVIDLQQDTEISKAGIRTNIVTGDW
                     ILDAENFIVSVSDDGKNFTPVAGIQTNLTDRAEHWKGVVTHTVTFNPVKTRYVKITVE
                     PFKAFPKWHGANGKPYIFVDEISIF"
BASE COUNT         1179 a         1064 c         1079 g         1594 t
ORIGIN      
        1 ccagtcgaag aatcttgtat ttaatacctg attcggtagt ttgaaattca gcattaacga
       61 gtggagtttt cagaacatcg attgtccagt tgaacgggaa actgtttttg tcgtttttat
      121 tcgcttccag ccagtcgttg ttcattactt tccagtccat gtatgcatct tgtttttcac
      181 aagctgagaa aatcaacgat aatgcagaaa tagccaatat taagagaatt tgattaagtt
      241 tcatatatgt ttggtagaaa aaataatttt ttattgttca aaaatgaaag cgtaaagata
      301 ctattttttt tcaattctga aagagtaact tccgattctg tttccatcca cgaagaaatc
      361 ggcctgatag gtaccgattt ggagaatttc ttccactttc cagtacactg cgtcgctgtg
      421 agcttcgccc gtgtaatcaa attctttttt cagtgagtat tcgatgtttt tgttctcata
      481 ggcgaaaacg ctatttcctt tctgcatcac ttcaccatcc gggcgcgaca gacgcagata
      541 tacagttttt ggtcccggtt cggcggttat gtttttgccg atggtgaagt tgaatttcag
      601 gtttgtcatt ttggacgaac gttccgtttt gcggtttcgt tcatccatcg gattgaatga
      661 aaaattatat agttcgagta cggctgcgcg ggttaccacc tcgctcagcg attctttctc
      721 ttttgccagc atttctgccg tttccgaaac gttttggtat cgttgcttaa cctccagatt
      781 ctcgttggtg agccgtttgt tcagggagtt taatgagtct atttgctgca cgtaatgtgc
      841 caggatagtt cttagtgtgg ctatttcagc tttcagttcg gctatgcgtt tggcatccgt
      901 ggctttggtg cttttcaatt ctgcctgcaa gtccttgatt ttttgcttct gcaggtcaaa
      961 ttcacggagc agcgaatcgt tgtgtacata catggtataa ccgtccattt cgccggcaag
     1021 cgcatccact tcagtaacca tctgctgctt ttcttcttcc ataaattgtt ctgtctgagc
     1081 tatttgctgg ctttgtttgt ttgatttgtt cataaaaata atcaaaccag ccaccagagc
     1141 ggctattact aatgtcagaa ctacaatcaa aatggtcagt ttctttttat ccatattttt
     1201 tcattttttt ttgaagcctc attctcttca gcacttttcc ctcacggaga agaaccgggc
     1261 aatcaaactt tttttactat tttattgtaa atgtgtactt taatagtccc tttttgggaa
     1321 atattcggag atttttcttt tacaattcgc tgatgacttt ctccagatga atgccgtgag
     1381 accctttgat gagtataaaa cggttgatca gcggattttt ctgaagatac tctttcagta
     1441 aatcaacatc tgcaaaatta ctaaaattat tccgggtagc attaaaattc ggccccacca
     1501 gaaaaacgtc cgtgaatccc gattcgttta ctaagtccac cactttctga tgctcttcgg
     1561 ccgattggct gcccagttcg agcatatcgc cgataatcag cgttttatgc ggtgcttcca
     1621 tctgggcgaa attcagaata gctgcctgaa gactcgtagg gttggcattg taggcatcga
     1681 tgatgagttt atttttgtct gtttcagtga attgcgaacg gttgtttctc ggctggtaat
     1741 tttccagacc ggctttaatc tgatcattgc ttacttccag ataatttccg atagctactg
     1801 cagccagcac attctcggca ttgtaagtgc cgattaattg ggttttcaca tcaaaatccc
     1861 cctctgacgt aacacattgc atcctgagca gcggactgca cgaagtaatg gtgcctgtag
     1921 cctgattgcc gttgatttca ctgtccgttt tataagctaa aatactgttt gcatctctga
     1981 aacccgattt ttcagccatt tttttcagat attcgtttcc ggtattcaga aatatttttc
     2041 cggatttttt cagtataaaa tcatacagtt cggctttggt attcattact ccttcgaaag
     2101 agccgaaacc ctccaggtga gctttgccca cattggtaat gatgccgtaa tcgggcagag
     2161 caatttcagc gagtattttg atttctcccg gatggttggc gcccatttcc actacggcaa
     2221 tatcgtgttc gggtttcagt tgcagcagtg tgagcggcac tccgatgtga ttgttgaaat
     2281 ttccccgggt aaaatgggtg ttgaatttct ccatcagtac ggatgcaatc agttcttttg
     2341 tagtcgtttt gccattggtt ccggtaatgc cgattatttt tgtacccagt ttttgccggt
     2401 gaaaacgtgc cagttcctgg aggctttcca gcacatcgtt aactacaata aatcgctcat
     2461 ccacagcata ttgcggattg tcaatcactg cataggcgca tcctttttca agagctccgg
     2521 cagcaaagcc attggcatca aagttatcgc ctttcagggc aaaaaatatt gaatcgggtg
     2581 gacaatttcg gctgtcggtg caaataagcg gatgtttcag aaagatatcg tagagtctca
     2641 ttcggaattt ttctgcaaaa ataatcaatc ttgcagactt gcacgaaaat atctgttttt
     2701 attaagattt tataaactaa aagcgtcagg tctttgtttc attggtagta aaaaccttca
     2761 ttcaaaaatc ggatggaaac tgaaaaagat aaaaaagaca aagtcattca ggtggtaaaa
     2821 gataaattct caaaattgcc tctcggattc attatcggag ctgctatcgg cggcggactt
     2881 tacctctatg ccaccaggag taaaggcact aaaagcgtga acgctattac caatatggcc
     2941 aagctggctg ctccgtatgt gattattgcc atgctggata agctggtgga tacgtatgaa
     3001 accagtcgca accgcgaaat agaggaagct gaagccgaag tgaattgatt ttcaaaacat
     3061 tagcagtaaa ataagaaaac gcattgccgc cagtggcaat gcgttttctg attttcttat
     3121 tcgtgtaatt cgctattatt agaatatcga aatttcatcc acaaaaatgt atggtttgcc
     3181 attcgctcca tgccatttgg ggaatgcttt gaagggctcg acggtgattt taacgtagcg
     3241 agtttttacc ggattgaatg taacagtgtg agtgacaact cctttccagt gttcggcccg
     3301 gtcggtaagg ttagtctgaa tacctgcaac gggagtaaaa tttttcccgt cgtccgaaac
     3361 agacacgata aagttctctg catccaaaat ccagtcgccg gtaactatgt ttgttcgaat
     3421 tccggctttt gaaatttcgg tatcttgctg caggtcaatt acggcaatca ggtcagttcc
     3481 ctgaaatccc atccagcgac ctgttttgta atttgtatcg ccggttagtc cgtcattgag
     3541 taatccggct cctgtgtagg tatagttccg atcaggagta gtgagcagcg taatcggctt
     3601 caggctcgat tttgataatt tcactttttc tgaaaaagta cggctgtttt tgccgttggc
     3661 ccggatggct ttggcgctga taacagcatc tttattcagg ctgattgttt cggtgtaccg
     3721 gatcgaattt gccgaaggtt cggtgccatc cagggtgtaa tagatgggtg cattatccag
     3781 agttgataaa atcacgtcca gtgttccttt gcccgtgttt ggcttgagat cggctttcac
     3841 gtcgtaaagg tgtgtggcat agttgtaatc aagtaaatca tacaggctga gtaagcgggg
     3901 tattctcctg agaaaatcag cgtaatcctt ttttgcaggc tgagtccact gtacgtcggc
     3961 caatgcagcc atacgcggca tggccatgta ttcaacctgc tgtgtggtgg ggatatactc
     4021 ggtccagata ttggcctgtg cgccaagtac cagtttctgt tcggccggag tgagttcttt
     4081 cgacatcggt tcgtagctgt aaaccagttc cacgggtaca tagccgccta tggcatcggg
     4141 ctcgtttttc gtgtcggttg tttggtaata atcaaaatac aggtagctgt ttggtgtcat
     4201 gatcacatca tgacccatgc gggcggcttc aatgcctccg gcttcgcccc gccatgacat
     4261 cacggtggca tttggagcaa gagtaccttc cagaatttcg tcccagccga taatccggcg
     4321 tcctttgccg ttcaggaatt tctccatccg ctccatcacg tagctttgga gataaaattc
     4381 ttttttgtgt tttttatcat ctttcaatcc cagttctttg atttttgcct ggcagtcggg
     4441 gcatttttcc cagcgtactt tcgggctttc gtcaccgcca atgtggatgt atttcgacgg
     4501 aaaaagttcg cacacttcgg tcagtacatc ttcgaggaat gtgtaggttt tttcacgtcc
     4561 ggcacaaagc acatcgtcaa atacacccca tgatttttcc acttcatacg gtccgcctgt
     4621 gcaaccgagt tccggatacg aagccagtgc tgccagcatg tggcccggaa ggtcaatttc
     4681 aggaataacc gtcacatagc ggtcctgagc atattttact atatcacgga tttcgtcctg
     4741 tgtgaaaaat ccgccgtgtg gtttgccgtc gaattttccg gtgttatgtc ctattaccgt
     4801 ttcgcttctc caggcaccca cttcagtcag tttcgggtat ttttttatct caatgcgcca
     4861 gccctgatct tcggtcaggt gccagtgaaa tttgttcatg ttgtgcaaag caatca
//
LOCUS       DAORKP010000155         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4061_length_4870_cov_10.625130, whole genome shotgun sequence.
ACCESSION   DAORKP010000155 DAORKP010000000
VERSION     DAORKP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_4870_cov_10.625130"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..368
                     /locus_tag="PLN75_08095"
     tRNA            297..368
                     /locus_tag="PLN75_08095"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:330..332,aa:Glu,seq:ttc)
     gene            407..661
                     /gene="rpsT"
                     /locus_tag="PLN75_08100"
     CDS             407..661
                     /gene="rpsT"
                     /locus_tag="PLN75_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRG04116.1"
                     /translation="MANHKSSIKRIRQTNTKKLANRYYAKTARNAVRKLRATTEKAAA
                     VELLPKVSSMLDKLAKKNVIHKNKAGNLKSKLTLYVNRLA"
     gene            complement(774..3608)
                     /gene="polA"
                     /locus_tag="PLN75_08105"
     CDS             complement(774..3608)
                     /gene="polA"
                     /locus_tag="PLN75_08105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRG04117.1"
                     /translation="MKRLFLLDAYAIIYRAYYAFIRNPRVNSKGLNTSAIFGFVNTLE
                     DVLRKEKPSHIAVAFDPPGKTFRHDEYESYKAQREASPEDIRKAVPIIKDIVRAYNIP
                     VLEVPGYEADDVIGTLAKEAERAGFEVFMLTPDKDYGQLVSDHIFMYRPRHTGGFEVM
                     GPEQVRQKYGLQSHEQVIDLLGLMGDSSDNIPGCPGVGEKTAVKLLQEFGSIDNMIIN
                     TDKLKGALKTKIEENIEQIRFSKYLATIKTDVPVTFDEEKLKLEPVNEPALRALFEEL
                     EFRTLSARMWNEVKAPVKSESKQPKASQGAQMSLFDTPETPENKSGNPDNAQYLSSET
                     ELTDAEGMRTIHNSPHQYTLVDDRVKRAGLVTKLFMQKSVCFDTETTGLDVFNAGLVG
                     LSFSFKTGEAYYVTLPPDRQACMEVLKEFKAFFQSEQIEKVGQNMKFDLLMLAQYGIE
                     LRGKLFDTMIAHYLLQPELRHGMDYLAEIYLKYRTIHYDDLFGGKPKTKQNILNVDLN
                     LLCDYASEDADITFQLKQILEKEIVENGLEKLFYEMEMPLMRVLAIMEQNGVRIDAGA
                     LRESSEILTKIMLALEKEIQQMAGTEFNVSSPMQVGEVLFGQLKLDEKAKKTKTGQYS
                     TSEDVLEKLKSRHPVVDKILEYRGLKKLLSTYIDALPLMVNPKTGKVHTSFNQTVAAT
                     GRLSSTNPNLQNIPVREELGREIRKAFIPDAGEVFLSADYSQIELRIMAHLSADVNML
                     EAFNSGNDIHAATAAKIFKVSLDEVTPDMRRKAKTANFGIIYGISTFGLAERLNIPRA
                     EAKELIDGYFETYPDVKKYMDNAIQTAKERGYVETIYKRKRYLADINSQNSVVRGYAE
                     RNAINAPIQGSAADIIKIAMVRIQKRLETENLRAKMTMQVHDELNFTVPEDEKSRVKE
                     LVVNEMQNVIKLKVPLIADCGIGDNWLEAH"
     gene            3793..4767
                     /locus_tag="PLN75_08110"
     CDS             3793..4767
                     /locus_tag="PLN75_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRG04118.1"
                     /translation="MNYIEKIKRPILAEMLEFEKIYAKVLDSDNNLLSNVHAYVMEGS
                     GKKLRPILTLLAAKLLGKINDETLYGAFSLELLHTASLVHDDVVDDTLERRGRASVNS
                     RWTNKIAVLTGDYLLSKSLHCAYSTGNIDIVRAISYIGMTLTDGELLQLANTKQSSTT
                     EKEYFEIIKRKTALLFATCTEVGALSVKANRDELEHLRNYGEYLGICFQIRDDIFDYY
                     EETHIGKPTGNDVRDGKLTLPLIYAIEHTSGSAKDEVVKMIDNKDFSAENINKIMHFA
                     HEKGGVEYATKVMDNFIQKARIELNDFPDNDAKNALIDCLEFVAERDF"
BASE COUNT         1323 a         1071 c         1062 g         1414 t
ORIGIN      
        1 aaatggttga caagcaaata ggagctacac ccaaagcatc cctgaaagca aaagtggaag
       61 cattactctg agcaattcaa cgtacaacag cctgaaagcg gaatgatttt ttatttatca
      121 tttcgctttt gggctgaata tatacggcaa accgacaact cgctgacaat aaacgatttg
      181 tgtgcaaaat aaataatttt gtaaaatatt tcatcacatc attttgtcaa ttcaaaaatc
      241 tgccgtatat ttgcacccgc aattgaaaca aaggatttta gttaaatcca ttcaatggcc
      301 gattcgtcta tcggttagga cgccagattt tcattctgga aagaggggtt cgattcccct
      361 atcggctact aaaaaaaatt attgattact caaaaagaca ttagagatgg caaatcacaa
      421 atcatccata aaaagaattc gtcagactaa caccaaaaaa ctggcaaacc gctactatgc
      481 taaaactgcc cgtaacgccg taagaaaatt gcgcgctaca acagaaaaag cagcagctgt
      541 agaattgctt ccaaaagtta gctccatgct cgacaaactg gcaaagaaaa atgttatcca
      601 taaaaacaaa gcaggtaatc tgaaatcgaa actgactttg tatgtgaatc ggttggcata
      661 acaaattgct tctaaaaaaa tacggaagcc ctttctcact atgaggaagg gcttttttgt
      721 cgtagtatgt tgtcctgaag gttgttttca gttttacaac gtgccggcgg cggtcagtgt
      781 gcctcgagcc agttatcccc tattccgcaa tcggctataa gaggcacttt cagctttatc
      841 acattttgca tttcgttcac caccaattct ttgaccctgc ttttctcatc ttcgggaacc
      901 gtgaaattca attcgtcgtg tacctgcatg gtcatttttg cgcggaggtt ttctgtttcc
      961 aaccgctttt ggattctcac catcgctatt tttataatat cggcagcgga gccctgtatc
     1021 ggagcattga tggcatttcg ctcggcatag ccgcgcacta cgctgttttg tgaattgatg
     1081 tcggccaggt atcgtttcct tttgtaaatc gtttccacgt atccgcgttc tttggccgtc
     1141 tgtatggcat tatccatgta ttttttcaca tcgggataag tctcaaaata cccatcaatc
     1201 agttctttgg cttccgcacg cggaatattg agccgctcgg ccagtccgaa cgtggagatg
     1261 ccgtaaataa tcccgaagtt ggccgttttg gctttgcggc gcatatccgg agtcacttca
     1321 tcaagtgata ctttgaagat tttagcggcc gtagcagcat ggatgtcgtt gccactgttg
     1381 aatgcttcga gcatatttac atcggcgctc aggtgggcca ttatacgcag ttctatctgc
     1441 gaatagtcgg cactcagaaa cacctcgccg gcatcgggta tgaatgcttt gcggatttcg
     1501 cgtcccagtt cctcgcgcac cggaatgttc tggaggttcg gattggtgga actcagccgg
     1561 cctgtggcag ccaccgtttg attgaaggaa gtgtgcacct ttccggtttt cggattcacc
     1621 atcagcggaa gtgcatcgat gtaggtgctc aacagctttt tcaaaccgcg gtattccaga
     1681 atcttatcca caaccgggtg cctggatttg agcttttcga ggacatcctc cgatgtggag
     1741 tattgtccgg ttttggtctt tttagctttt tcatccagtt tcaactgtcc gaaaagcacc
     1801 tcgcccacct gcatgggcga cgaaacgttg aattctgttc cggccatttg ttgaatttcc
     1861 ttttcaagag ccagcattat tttggtcaga atttcagaac tttcgcgcaa ggcacccgca
     1921 tcaatccgta caccgttttg ttccataatg gcaagaaccc gcatcagcgg catttccatt
     1981 tcgtagaaca gcttctccag gccgttttca actatttcct tttccagtat ttgttttagt
     2041 tgaaaagtaa tgtcggcatc ttcggatgca tagtcacaga gcaggttcag atcaacattc
     2101 aggatatttt gcttcgtttt gggctttcca ccgaataaat cgtcgtaatg gatggtgcgg
     2161 tatttcaggt agatttccgc cagataatcc atgccgtgtc tcaactccgg ttgaagcagg
     2221 tagtgggcaa tcatggtgtc gaacaacttt cctctgagtt caatgccgta ttgtgccagc
     2281 atcagcaggt cgaatttcat attttgacct actttctcta tttgttcgct ttgaaaaaat
     2341 gctttgaatt ccttcaacac ctccatacat gcctgtctgt cgggtggaag tgtaacgtaa
     2401 taagcttcac cggttttgaa tgaaaatgac aaaccaacca atcctgcatt gaaaacatcc
     2461 agtccggtgg tttcggtatc aaagcaaacc gacttttgca taaaaagttt agttaccagt
     2521 ccggctcttt tcactctgtc gtccaccagc gtgtactgat ggggtgaatt atgaatggtt
     2581 ctcattcctt cggcatcggt cagttccgtt tctgaagaca gatattgtgc attatcggga
     2641 tttccggatt tgttttcagg ggtttcgggg gtatcgaaga gcgacatctg agcaccctga
     2701 cttgcctttg gttgttttga ttcgcttttg acaggtgctt tcacttcgtt ccacatcctg
     2761 gccgagagag ttcggaactc gagctcttcg aacaatgctc gcagggccgg ctcgtttaca
     2821 ggttcaagtt tcagtttttc ttcgtcaaaa gttaccggaa catccgtttt gatggtagcg
     2881 aggtattttg agaaccgaat ctgttcgata ttttcttcga ttttcgtttt cagggcacct
     2941 ttcagtttgt cggtgtttat tatcatatta tcgatgctgc cgaattcctg cagcaatttt
     3001 acggccgttt tttcgcccac tcccgggcat ccgggtatat tgtcggaact gtcgcccatc
     3061 aatccaagca ggtcaatgac ctgctcgtga ctctgcaacc cgtatttctg ccttacctgt
     3121 tcgggtccca tcacctcaaa tccaccggta tgtctggggc gatacataaa aatatgatcc
     3181 gagactaatt gcccgtaatc tttgtcgggt gtgagcataa aaacctcaaa accggccctt
     3241 tcggcttctt tggccaatgt acctatcaca tcgtcggcct catagcccgg cacctccagc
     3301 acggggatat tgtacgcccg cacaatgtct ttgatgatgg gcaccgcttt tcgaatatcc
     3361 tcgggcgagg cttcacgctg agctttgtaa ctctcgtatt catcatggcg gaaggttttt
     3421 ccgggaggat cgaaagctac ggcaatatgc gaaggttttt ccttcctcaa tacatcttcc
     3481 agcgtattga caaatccaaa aatagccgaa gtattcagcc ccttggaatt gacacgcgga
     3541 tttcggataa aggcgtagta tgcacggtaa ataatagcgt aggcatcgag caaaaagagt
     3601 cgtttcatct gattaaattt gaaaaagtaa atggaaagaa cttaacataa aaacataatc
     3661 atgtaataat tgttttcatt caaagtctgg tgatgtaaaa ttacacaaaa aaaacttatt
     3721 tatttctgaa aggataaaag aaagaacaaa attgcttaat tttgcatact gtgtcaaaac
     3781 ttctatagct aaatgaacta tatagaaaaa atcaagcgtc cgattctggc tgaaatgctg
     3841 gagtttgaga aaatatatgc caaagtcctt gacagcgaca ataatctgct aagcaacgtt
     3901 catgcctatg tgatggaagg cagcggaaaa aaattacgtc ccattcttac gctgctcgca
     3961 gccaaactac tcggaaaaat caacgacgaa acactttatg gcgcattctc acttgaattg
     4021 cttcacacgg caagtctggt gcacgatgat gtggtggacg acactcttga aaggcgcgga
     4081 agagcatcgg tcaattcccg atggaccaat aagatagctg tactgaccgg cgattatctg
     4141 ctttcgaaat ccctgcactg cgcctactct accggcaata tagatattgt acgggcaatt
     4201 tcatacatcg gaatgacact taccgacggc gagctacttc aactggctaa tacaaagcaa
     4261 tcttcaacaa ccgaaaagga gtattttgaa ataatcaaaa gaaaaacggc gctgcttttt
     4321 gccacttgta ctgaagtggg agcactttcc gtaaaagcca accgtgatga gctggaacat
     4381 ctgcgtaatt acggagagta tttgggaatc tgttttcaaa tcagggatga tatttttgat
     4441 tattacgaag aaacccacat aggcaaacct acaggcaatg atgtgagaga cgggaaactg
     4501 accttaccac tcatttatgc aattgaacat acatcgggca gcgcaaagga tgaagtggta
     4561 aaaatgattg acaacaaaga cttttcggcc gaaaacatca ataaaataat gcattttgct
     4621 cacgaaaaag gcggcgtgga gtatgctaca aaagtgatgg acaacttcat acaaaaagca
     4681 cggatcgaac tgaatgattt ccctgataat gatgcaaaaa atgcattgat agactgtctg
     4741 gaatttgttg ctgaaagaga tttctgattg gttgtttgag tgtttggatg ttcggttgtt
     4801 tggatgtttg gggtagaatg gttgaattgg cttactgtag tagagtggag ttgataacta
     4861 aacaaatctg
//
LOCUS       DAORKP010000156         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4188_length_4791_cov_7.468750, whole genome shotgun sequence.
ACCESSION   DAORKP010000156 DAORKP010000000
VERSION     DAORKP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_4791_cov_7.468750"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1675
                     /locus_tag="PLN75_08115"
     CDS             245..1675
                     /locus_tag="PLN75_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG04119.1"
                     /translation="MISEKIKLREKIGYGFGDAASSMFWKIFGMYSLFFYTDVFGITA
                     AAAGTMFLVARLWDSFFDVLVGIMSDRTKSRYGKYRPYLLWFAIPFAVMGAVTFFVPD
                     FGQTGKLVYAYITYSLMMIVYSLINVPYASLLGVISSNPQERNSLSSYRMSFAFIGSF
                     VTFMLLQPLIDFYAKTFNPSDVALNAVNSTVSTSPVGWVLGVGTIGMICIVLFLLCFS
                     WTKERVTQIESEENVSVKQDLKNLFHNAPWWILVGTGLAALLFNAIRDSVAIYYFRDY
                     VSINYRMPGTGWDMTTIYFLVGQAANLIGVMLAPTVSARFGKKKTYMIAILLAGFFSI
                     GMYLIPNNITYILILQFVISIFAGYVLPLLWSMFADIVDHQELKTGRRATGLIFSSSS
                     MSQKLGWALGAAMSGWILAIFHYMPDAAQQSAETIFGERIMISLFPAVCAVIAFVGML
                     FYPLSDKKVKEISEELNKKRELSGKK"
     gene            1698..4175
                     /locus_tag="PLN75_08120"
     CDS             1698..4175
                     /locus_tag="PLN75_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRG04120.1"
                     /translation="MQYGHFDDSRREYVITNPRTPWPWINYLGNEDFFSLISNTAGGY
                     SFYKDAKFRRITRYRYNNVPMDSGGKYFYINDGESVWNPGWKPCKTELDCYECRHGMN
                     YSIIKGSKNGVEAEALYFVPLKTHAEVQRLRLTNYSTETKNLKIFSFVEWALWNAATD
                     MENFQRNFSTGEVEIEDSVIYHKTEYRERRNHYAYYSVNAALNGFDTDRETFFGLYNG
                     FDEPQTVLEGAPRNSEAHGWSPIASHYVEVTLAPGESKDLIFMLGYVENEQEDKWESK
                     NIINKTKAKACIARFDTTDKVEAALAELRAYWDNLLSVFSATTHEEKLDRMVNVWNQY
                     QCMITFCFSRSASFFESGIGRGMGFRDSNQDLIGFVHQIPERARERIIDIASTQFPDG
                     GCYHQYQPLTKKGNNEIGQGFNDDPLWLILGTTAYIKETGDFGILDEKVPFNDSDGKD
                     ESNLTSPPSEGLGEASLFDHLTVSFNHVINHLGPHGLPLIGRADWNDCLNLNCFSNDP
                     NESFQTTENKTEGSKAESVMIAGLFVVYGRDYVELCQKSGKTAEAQRAQNEVDKMVAA
                     IKQHGWDGEWFLRAYDFYGNKIGSNENEEGKIFIESNGWCTMAGVGLDEGMVDKALES
                     VKERLDCEHGIVLNNPAFSKYYIEYGEISSYPAGYKENAGIFCHNNPWIMIGETVRGR
                     GDRAWEYFCKICPSYTEEISDLHKVEPYVYAQMIAGKDAFRPGEAKNSWLTGTAAWNF
                     YTISQYILGIQPTYDGLRIEPCIPADWKTFSVKRKFRGAQYHIEVKNPNGKMKGVSKI
                     LINGVGIADNVIPLFEAGNVINVEVIM"
     gene            4224..>4791
                     /locus_tag="PLN75_08125"
     CDS             4224..>4791
                     /locus_tag="PLN75_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRG04121.1"
                     /translation="MKKIKIEESNLKSPKGDLGDLKQQLTDELTQNILHFWQTKMKDK
                     NGGFFGQMTGQNELQKSAARGGILNARILWTFSSAARIFGNDAYKAIAEHARNYIFEH
                     FFDAEFGGTYWMLNADGSVADAKKQIYSQAFFIYALVEYYRLTNDEECLERAKELFYL
                     IEKNSFDKQKNGYFEAYSRDWQLLDDLRL"
BASE COUNT         1401 a         1014 c         1095 g         1281 t
ORIGIN      
        1 tcagggatgt gtgggagaaa aaagacctga ctttcaagcg taaaaagtat cagtcggact
       61 ttgtagtggg tggcgatgat attgtaatgt ttgtgataag tcctgcgatg aaaaaataac
      121 acccagttta gaaacatatc cacactgttc tgtttattca aataaaataa ttttgtaaaa
      181 tacaaaaatt acagtgacat cattcaatta atctatatca ataaaactaa taaaatcagg
      241 taaaatgatc tcagaaaaaa tcaaactcag agagaaaatc ggatacggct ttggcgacgc
      301 cgcttcttcc atgttttgga aaattttcgg aatgtattca ctgtttttct acaccgatgt
      361 ttttggaata accgctgccg ccgccggaac catgtttttg gtagcccgtc tttgggattc
      421 cttcttcgat gtgctggtag gcattatgag cgaccgcacc aaatccaggt acggaaaata
      481 tcgtccttat ctgctttggt ttgccatacc ttttgctgtc atgggtgcag tcaccttttt
      541 tgttccggat tttggccaga caggtaaact ggtatatgca tacatcacct attcgctgat
      601 gatgatcgta tattctctta tcaatgtgcc ctacgcatca cttttgggtg ttatttcatc
      661 caatcctcag gagcgaaatt cactgtcgtc gtaccgcatg tcgtttgcct ttatcggaag
      721 tttcgtaaca ttcatgctgt tgcagccact gattgatttt tatgcaaaaa cgttcaatcc
      781 ttcggatgta gctttaaatg ctgtgaacag tacagtgagc acctctccgg tgggttgggt
      841 gctcggtgtg ggaacaatcg gaatgatttg tatcgtcctg tttttgctgt gtttcagctg
      901 gaccaaagaa cgtgttactc aaatagaaag cgaagaaaat gtatcggtta aacaggattt
      961 gaaaaatctg tttcacaatg ctccatggtg gattctggtg ggtaccggac tcgcagcatt
     1021 gctattcaat gccatccgcg acagtgtggc tatttactat ttccgggatt atgtaagtat
     1081 caactaccgg atgccgggca ccggatggga tatgacgacc atttattttc ttgtaggtca
     1141 ggcagccaac cttattggag taatgcttgc tccgactgtt tcagcacgat ttggtaagaa
     1201 aaaaacctac atgattgcaa ttctgttggc gggatttttc agtataggta tgtatctgat
     1261 accaaacaac atcacatata tacttatttt gcagtttgtc atttcaatat ttgcgggtta
     1321 tgttctgccg ttgttgtggt ctatgtttgc cgacattgtt gatcatcagg aactcaaaac
     1381 cggacggcgt gcaaccggcc tgattttctc atcatcctcc atgtcgcaaa agctgggctg
     1441 ggctttgggt gcagccatga gcggctggat actggccatt ttccattata tgcctgatgc
     1501 agctcagcaa agcgctgaaa ccattttcgg cgaacgcatc atgatcagcc ttttcccggc
     1561 tgtatgcgct gtcattgcat tcgtgggcat gttattctac ccgctttcag ataaaaaagt
     1621 gaaggaaatt tcagaagaac tgaataaaaa acgtgaatta tccggaaaaa aataaccatt
     1681 caattaaaaa cgaaaaaatg caatacggac atttcgatga cagccggcgt gaatacgtga
     1741 ttaccaaccc gcgcacgccg tggccctgga taaattatct gggaaatgaa gattttttca
     1801 gcttaatttc gaatactgct ggtggatatt ctttttataa ggatgccaaa ttccgccgta
     1861 tcacccgtta taggtacaac aatgtgccca tggacagcgg tggaaaatat ttttatatca
     1921 acgacggaga atcagtctgg aatccgggtt ggaagccttg taaaaccgaa ctcgattgtt
     1981 acgaatgccg tcacggcatg aattacagca ttataaaagg ctcaaaaaac ggcgtggaag
     2041 ccgaagcact ttattttgtg ccgctaaaaa cacatgccga agtgcaacgt ttacgactga
     2101 caaattattc caccgaaaca aaaaacctga aaatattctc tttcgtggaa tgggcgctct
     2161 ggaatgccgc cacggatatg gagaattttc aacgcaattt ttcaaccgga gaggtggaaa
     2221 ttgaggattc ggtaatttat cacaaaaccg aatacagaga gcgccgcaac cattatgcct
     2281 attactcggt gaatgcagcc ttgaatggtt ttgacaccga ccgcgaaaca ttttttggtt
     2341 tgtacaatgg tttcgacgaa ccgcaaacgg tgctcgaagg tgcaccacgc aactccgaag
     2401 cgcatggctg gtcgcccatt gcttcgcatt atgtggaagt gacacttgcg ccgggcgaaa
     2461 gtaaagacct gatttttatg cttggatatg tggagaacga gcaggaagat aaatgggaaa
     2521 gcaaaaatat cataaacaaa acaaaggcga aagcctgcat tgcccgcttc gataccactg
     2581 ataaagtaga agctgctctg gctgaattgc gtgcctattg ggacaatttg ctgagcgtat
     2641 tcagcgcgac gacccacgaa gaaaaactcg accggatggt aaatgtgtgg aatcagtacc
     2701 aatgcatgat taccttctgt ttctcgcgtt cagcttcgtt tttcgaaagc ggtatcggcc
     2761 gtggcatggg tttccgcgat tctaaccagg atttgatagg ttttgtgcac cagattcccg
     2821 aacgcgcccg cgaacgcatc atcgacattg cttccacaca atttcccgat ggcggttgct
     2881 accatcaata tcaaccattg acaaaaaaag gtaacaacga aatcggtcag ggattcaacg
     2941 atgacccatt atggctgata ttgggtacaa cggcttatat caaggaaact ggcgatttcg
     3001 gcattctgga cgaaaaagta ccattcaatg attctgatgg taaagacgaa agtaacctta
     3061 catcccctcc ttcggagggg cttggggagg cttctttatt tgaccacctc accgtttcgt
     3121 tcaatcatgt gattaatcat cttggtccgc acggattacc gcttatcggt cgtgccgact
     3181 ggaacgattg tctgaatctg aactgctttt cgaacgaccc aaacgagagt ttccaaacca
     3241 ccgaaaataa aactgaaggc tccaaagctg aaagtgtgat gatagccgga ctttttgtag
     3301 tgtacggacg agactacgtt gaactttgtc aaaaatcagg caaaaccgcc gaggcgcaac
     3361 gtgcacaaaa tgaagtggat aaaatggtag cagccatcaa acaacatggc tgggatggcg
     3421 aatggtttct gcgcgcttac gatttttatg gcaacaaaat tggctccaac gaaaacgaag
     3481 agggcaaaat attcatcgaa tcgaacggct ggtgcaccat ggccggcgtc ggactcgatg
     3541 aaggcatggt ggacaaggcc ctcgaatcgg taaaagagcg tctcgattgc gaacatggca
     3601 ttgtgctcaa caatcctgct ttttcaaaat actatatcga atacggcgaa atatcgagct
     3661 atcctgctgg ttacaaggaa aatgcaggga ttttctgtca caacaatccc tggataatga
     3721 ttggtgaaac tgtgcgcggc cgtggcgaca gagcgtggga gtatttctgc aaaatctgtc
     3781 cttcatacac agaagaaatt agcgatctgc acaaagttga accttatgtg tacgcacaaa
     3841 tgatagccgg aaaagatgct ttcagacccg gcgaagccaa aaactcgtgg ctcacaggta
     3901 cggcagcgtg gaacttttac accatttcgc aatacatact tggcatacag cccacttacg
     3961 atggcctgcg catcgagccc tgcattccgg ccgactggaa aactttcagc gtaaaacgca
     4021 aattcagagg ggcacagtat cacattgaag tgaaaaatcc gaacggaaaa atgaaaggcg
     4081 taagcaaaat ccttattaac ggggttggaa ttgctgacaa tgtaattccc cttttcgaag
     4141 ccggaaacgt gataaatgta gaagtaataa tgtaactccc caatcccctg aagtggaagt
     4201 taacagataa ttgagattaa aaaatgaaga aaataaaaat agaagaaagt aacttaaagt
     4261 cccccaaggg ggatttaggg gacttaaaac agcaactcac cgacgaactt acgcagaata
     4321 ttcttcattt ctggcaaaca aaaatgaagg acaaaaatgg tggttttttc ggacaaatga
     4381 ccggccaaaa cgaattgcag aaatctgcag cccgcggtgg cattctgaat gcccgtattc
     4441 tctggacttt ctcatcggct gcccgtattt ttggcaatga cgcatacaaa gccattgctg
     4501 agcatgccag aaactatatt ttcgaacatt ttttcgacgc tgaatttggt ggaacttact
     4561 ggatgctgaa tgccgatggc tctgtggccg atgctaaaaa acagatttac agtcaggcgt
     4621 tttttattta tgctttggtg gaatattatc gccttacaaa cgatgaggaa tgtctcgaac
     4681 gagccaaaga gttgttttat ttaatcgaaa aaaacagttt tgataagcag aagaatggct
     4741 atttcgaagc ctacagccgc gactggcaat tgctcgacga cctccgactg a
//
LOCUS       DAORKP010000157         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4194_length_4786_cov_8.949905, whole genome shotgun sequence.
ACCESSION   DAORKP010000157 DAORKP010000000
VERSION     DAORKP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_4786_cov_8.949905"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2843)
                     /gene="ileS"
                     /locus_tag="PLN75_08130"
     CDS             complement(<1..2843)
                     /gene="ileS"
                     /locus_tag="PLN75_08130"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRG04122.1"
                     /translation="MKNFKEYSGLNLSEVNKKILEYWNEHDMFRKSVEIREGHPSFVF
                     YEGPPSANGMPGIHHVMARTIKDVFCRFKTMQGFQVKRKAGWDTHGLPVELGVEKALG
                     ITKEDIGKTITVEEYNAACRHDVMKYTREWEDLTEKMGYWVDMNDPYVTYDNRYIETL
                     WWLLKQLYNKGLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTVVAQFRLSPS
                     LSKGEGVARNLNEKLMQIFALNAPPSEDLGGLYFLAWTTTPWTLPSNTALAVGANIDY
                     VLVKTQNPYTKEKVSLILAENLLSSVMGKNEYEVLGKCKGKDLEGIEYEQLIPWVNPG
                     GKAFEVVIGDFVTTEDGTGIVHIAPTFGADDDRVAKQNGISPLILIDKDGNRRPMVDL
                     TGKFYKIEDLDADFVKNSVNTDLYAEFAGRFVKEEYKDSPTSPKEGLNSPPSGEPEGA
                     LDVDLAVMLKQQGLAFKIEKHIHSYPHCWRTDKPVLYYPLDSWFIKSTACREEMIALN
                     NTINWKPQSTGTGRFGKWLENLQDWNLSRSRYWGTPLPIWRTEDGTEEICIGSVEELM
                     DEIEKSIQAGWMAENPFKNFKVGEYTAENYDTKNIDLHRPYVDNIILVSATGKPMKRE
                     TDLIDVWFDSGAMPFAQLHYPFESLSQTLSEGKGLSEQPKKKGFGYTTADPALYEILK
                     ENAKKHKENPTQAEEVLWSYLKGKKLNGYKFRREHIIYDMIVDFVCLKEKLVIEVDGK
                     YHEDDDVKKLDQLKTQILKERGLRVIRFGNEEVLGNIEGVLDKIINELKQSHHVAPFP
                     SGRVGDGLSGLFPADFISEGVDQTRGWFYTLHAISAMIMGSVAFKNVISNGLVLDKSG
                     NKMSKRLGNAVDPFSTIENYGSDPLRWYMMTNASPWDNLKFDIEGVEEVRRKFFGTLY
                     NTYSFFALYANVDNFSAADAEIPVSERPEIDRW"
     gene            3312..4691
                     /gene="radA"
                     /locus_tag="PLN75_08135"
     CDS             3312..4691
                     /gene="radA"
                     /locus_tag="PLN75_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRG04123.1"
                     /translation="MAKTRSVYVCQNCGVDSPKWIGKCPSCGEWNTFVEEIIHKENSS
                     SKISMAGFEPIKQKPVLISSVETQAEERTDTSCHELNRVLGGGLVNGSLVLIGGEPGI
                     GKSTLVLQVVLGMKGKRTLYVSGEESVNQLKMRAERLSHKDPDCYIVSETSLEQLFVH
                     AQNVKPDLLIVDSIQTITTESIESSPGSVSQVRECAAALLRFCKTTNIPVLLIGHINK
                     EGSIAGPKVLEHIVDTVLQFEGDQHYMYRILRPIKNRFGSTSELGIFEMQQSGLREVS
                     NPSELLLSQNHEGLSGVAIASAIEGIRPFLIEVQALVSSAAYGNPQRSSTGFDLRRLN
                     MLLAVLEKRAGFKLAQKDVFLNIAGGIKVNDPAIDLAIISAILSSNIDIAIEQHVCMA
                     GEVGLSGEIRPINRLEQRIHEAEKLGFRKMIIPQVNLKSLDLKKINIELMQVRKVEEA
                     FRMLFGKNK"
BASE COUNT         1277 a         1084 c         1060 g         1365 t
ORIGIN      
        1 atccagcggt caatttcagg acgttccgaa accggaattt cggcatcagc tgccgagaaa
       61 ttatctacgt tggcatacag tgcaaagaac gagtaggtat tgtaaagtgt accgaagaat
      121 ttgcggcgca cttcttccac accttcaata tcgaatttca ggttgtccca gggcgatgcg
      181 ttggtcatca tgtaccagcg gagcggatcg gaaccgtaat tttcgatggt agaaaacggg
      241 tcaacggcgt taccaagccg ttttgacatt ttattgccgc ttttatcgag caccaatccg
      301 ttggaaatca catttttgaa ggcaacggat cccatgatca tcgcactgat ggcatgtaac
      361 gtgtagaacc agccacgtgt ctggtcaaca ccctcggaga tgaagtcggc agggaaaagg
      421 cccgaaagcc catccccaac ccttcccgaa gggaagggag caacatggtg cgattgctta
      481 agttcattaa taatcttatc aagtacaccc tcgatatttc ctaatacctc ttcattacca
      541 aaacggatta cccgtaatcc tctttctttc agaatctgtg tctttaactg gtctaatttc
      601 ttaacatcgt catcttcatg atattttcca tcaacttcta tgaccagttt ttccttcaga
      661 cagacaaaat ccacaatcat atcatagatt atatgttctc ttctgaattt atagccattt
      721 aattttttac ccttcagata cgaccaaaga acttcttctg cctgagtcgg attttctttg
      781 tgtttctttg cattctcttt taatatttca taaagagccg ggtctgccgt cgtataccca
      841 aaacctttct tttttggctg ttcgcttaag ccctttcctt cggaaagggt ttgggatagg
      901 ctctcaaacg gataatgtaa ctgtgcaaaa ggcatagctc cgctgtcaaa ccacacatcg
      961 atcaggtctg tttcgcgctt catcggtttg cccgtagcgg ataccagaat gatattatcc
     1021 acgtacggac gatggaggtc tatatttttt gtatcgtagt tttcggcagt gtactctccc
     1081 actttgaaat ttttgaacgg attttctgcc atccatccgg cctggatgga tttttcaatc
     1141 tcatccatca attcttccac cgatccgatg catatttctt ctgtaccatc ttctgttcgc
     1201 cagatgggca gcggtgtacc ccagtaacgg ctgcggctca gattccagtc ctgcagattt
     1261 tcgagccatt tcccgaaccg gccggttccg gtcgattgcg gtttccagtt gatggtgttg
     1321 ttgagtgcta tcatctcctc gcggcaggct gtgcttttta taaaccagct gtcaagcggg
     1381 taataaagca ccggtttatc cgttcgccag cagtgtggat aactgtgaat gtgtttttca
     1441 atcttgaatg ccaaaccctg ctgtttcagc ataactgcca aatcaacatc caaagccccc
     1501 tccggctccc ccgaaggggg agagtttagc ccctccttcg gagaggttgg agaatcttta
     1561 tattcctctt ttacaaaacg tccggcaaac tcagcataca agtctgtatt gacactattt
     1621 tttacaaaat cagcatccaa atcctctatt ttgtagaatt ttcctgtcaa atctaccatt
     1681 ggacgtctgt tgccgtcttt atcaatcaaa attaacggcg aaataccgtt ttgcttagcc
     1741 acgcggtcgt catcagcacc aaatgtggga gcaatgtgca caattccggt tccgtcttcc
     1801 gtagttacga aatcgccgat aacgacttcg aaagcttttc cacccggatt tacccaggga
     1861 ataagctgct catattcaat tccctccagg tcttttcctt tgcatttacc gagtacttcg
     1921 tactcgtttt tacccataac agatgaaagc aggttttcag ccaaaatcag actaactttt
     1981 tctttggtat atgggttttg agtttttaca agcacgtaat caatgttcgc ccctactgcc
     2041 aatgcggtat tggaagggag tgtccaggga gtagttgtcc aggcgagaaa atacaagccc
     2101 cctaaatcct ccgaaggagg ggcattgagt gcaaaaatct gcattagctt ttcgttcaaa
     2161 ttacgagcaa ctccctctcc tttggagagg gatggggaga ggcgaaactg cgctaccacc
     2221 gttgtatctt tcacatcgcg gtaacagccg ggctggttca gctcgtgggt ggaaagtccg
     2281 gtgcctgcag ccggtgaata cggctgtatg gtgtagcctt tatagagtaa tcccttgttg
     2341 tacagctgtt tgagcaacca ccagagtgtt tcgatgtacc ggttgtcgta tgttacatac
     2401 ggatcgttca tatccaccca gtagcccatt ttctcggtca ggtcttccca ttctctggtg
     2461 tatttcatta catcgtggcg gcaggctgcg ttgtattctt ccacggtgat ggttttcccg
     2521 atatcttctt tggttattcc cagggctttc tccacgccca gttccaccgg aagtccgtgg
     2581 gtatcccatc cggctttccg ttttacctga aatccctgca tggttttgaa gcggcagaac
     2641 acatccttga tggtgcgcgc catcacatgg tgaatgcccg gcatgccgtt ggccgaaggc
     2701 ggaccttcat aaaacacaaa tgaagggtgt ccttcgcgga tttcgacact cttacggaac
     2761 atgtcatgct cgttccagta ttcgagtatc tttttgttta cttccgaaag attaagaccg
     2821 gaatattctt taaaattttt catacagaca acatgaattt acaaaagtca ttcagtaagt
     2881 gaaatgacct gttaatcaaa aattgggtgc aaaattacaa aaaaaatatc attccggaaa
     2941 gcaggttaag atgctgaaaa ttatcgcctg taattctgaa aacagtagat tacaaatacc
     3001 tgcagcctgt gatattcaaa tcaggtgttt ttatttacca aattcaattc agggatgttc
     3061 aaaataaaaa tcattttcat ccggcaaaca ataaataaat cagatttctc actccattcg
     3121 aaatgacaat taacatttta tctttttgga gggttgttgg ttggcgtttt cgccgccaac
     3181 caacaaccct cctccagtaa cgcaggttaa atccttgtca acctgagcat agcgagggat
     3241 cttataattt tcacagaaca atagtaaata aaaaatttaa aaagttatct ttgtgtaaaa
     3301 aataatgctt tatggctaaa acaagatccg tctatgtgtg tcagaactgc ggggtagatt
     3361 ctccgaaatg gattggtaaa tgtccgtcgt gcggcgagtg gaatacattt gtagaagaaa
     3421 tcattcacaa ggaaaacagt agttcaaaaa tatcgatggc cggttttgaa ccgataaaac
     3481 aaaaaccggt gcttatatcc agcgtagaga ctcaggctga agaacggaca gataccagtt
     3541 gtcacgagct aaaccgggta ctgggaggag gattggtgaa tggttcgctg gtactgatag
     3601 gcggcgaacc cggcatcggg aaatccacgc tggtattgca ggttgtactc ggtatgaagg
     3661 gtaaacgaac gctttatgta tcgggcgagg aaagtgtcaa tcagctgaag atgcgtgccg
     3721 aacgactttc gcacaaagat ccggattgct acattgtaag cgaaacttcg ctcgaacagc
     3781 tctttgttca tgctcaaaat gtgaaacccg atttacttat cgtagattcc atccagacta
     3841 ttaccaccga atccattgaa tcttcgccgg gcagtgtatc gcaggtacgc gagtgtgcgg
     3901 cagcgctgct gagattttgt aaaacaacca atattcctgt attactgatc ggacatatca
     3961 acaaagaggg cagcattgca ggtcctaaag tactcgaaca cattgtggat acggtattgc
     4021 agttcgaagg cgatcagcat tacatgtacc gcattctgcg tcccatcaaa aaccgttttg
     4081 ggagtacctc ggagctgggc atattcgaga tgcagcaaag cgggctacgc gaagtgagca
     4141 atccgtcgga attgctgctc tcgcagaacc acgaaggcct gagcggagta gccattgcat
     4201 cggccattga aggcattcgt ccttttctga ttgaggtaca ggctttggtg agttcagccg
     4261 catacggcaa tccgcaacgt tcgagcacgg gttttgatct tcgcaggttg aatatgttgc
     4321 tggcggtatt ggaaaaacgc gcgggattta aattggctca aaaggatgtt ttcttaaata
     4381 ttgcgggtgg aattaaagta aatgatccgg caattgattt ggcaataatt tctgcaattt
     4441 tatcatcaaa tatcgatatc gccatcgaac aacacgtttg tatggcaggt gaagtggggc
     4501 tttccggtga aattcgtcct atcaaccggc ttgaacaacg catccacgaa gccgaaaaac
     4561 tgggattcag gaaaatgatt atcccgcagg taaacctgaa aagcctggat ttgaaaaaaa
     4621 tcaacatcga actcatgcag gtgagaaaag tggaagaggc tttcagaatg ttgtttggaa
     4681 aaaataaatg aaaatataaa aaccgaatgc tttgcaacat tcggtttttt tgtgggcgtt
     4741 gacggattcg aaccgccgac ccctccgata tatcggagtg ttctga
//
LOCUS       DAORKP010000158         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4285_length_4738_cov_9.850950, whole genome shotgun sequence.
ACCESSION   DAORKP010000158 DAORKP010000000
VERSION     DAORKP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_4738_cov_9.850950"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..2602
                     /locus_tag="PLN75_08140"
     CDS             98..2602
                     /locus_tag="PLN75_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HRG04124.1"
                     /translation="MRNSFYISFLIVLLILTYSPLYAVPAYPFPQQILQPDGTSLSVI
                     LKGDEFHHYHVTEDGYFIKRGINGVFNYAIPDEYGRLQDTKIKANDPNKRESAEKSFV
                     SALQVNQSLQRINKEARVERTPSSTNAGQKSQFPRVGSPKSLVILVNFKDTSFVTPNP
                     KMAFSALLNEKGYSANGGTGSARDYFIDNSSGKFSPEFDVVGPFTLPQRMSFYGENGT
                     NDSDKNPVQMIVDACKAADDAGVDFSVYDTENDGIVDNVFVYYAGYNEAEWGGVNTIW
                     PHRWGIYPTSIYNGGNYGGSAASVTFDGKRVEDYACTSELRGKTGTNMAGIGTFTHEF
                     GHVIGLADMYATNGAKHHTLSYWDIMDAGAYLNQGRTPPAYNTFERMQLGFITPYILT
                     GPEDVKLQPLTTHNQAYLISPSDVHNLNGNNPSPVEYFLLENRQKVGWDAYLPGHGML
                     IYRIYYNQNDWYYNQPNNDPLKMGVDIIEADKTANTTSFAGDPFPGTSNVDSYIPALR
                     SGTELNKPITSIIESDKTIIFRYKGGKNVPVLSAESNFTPASTVQGTPSESQSITVAG
                     IRLVSDMIISFVQNEHFEVKKETDPESAWAKSLKLTPVDSVVTPIKILVRYNPTVPSY
                     RIMHSDMMNITSVYAATISKNVTGKSTRPVYVVEPTATKESDLTYKGFVANWNPVFDA
                     TGYYLTVIETNAAGEQSPIFTDKWVETTSYTLYHLISGRNYFYKVKASDKNLQFEYEN
                     ITGYSNTVQVKTPDYPYEKELRAVVSKGSVMVFVPSDDKSAGEPGAIKTVDIFNTMGQ
                     KVKSYPADRDIVEISDLPRGMVFIIQSGKYRTKVIL"
     gene            complement(3013..3345)
                     /locus_tag="PLN75_08145"
     CDS             complement(3013..3345)
                     /locus_tag="PLN75_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRG04125.1"
                     /translation="MGKFVVTQRTNGDFQFSLKAGNGQIILSSQGYAAKPSCLNGIES
                     VRKNSQDDARFDRKVSSNGKPFFNLTATNGQIIGNSEMYESEAARDNGIESVKKNAPD
                     ADLDDQTL"
     gene            complement(3547..3966)
                     /locus_tag="PLN75_08150"
     CDS             complement(3547..3966)
                     /locus_tag="PLN75_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRG04126.1"
                     /translation="MTLKEKYAALIDAANQSGATNLQVVEQDGVLYINGDVPSGAAKD
                     HLWDVYGTIDPNFAAGDVVMNVNAAVVEGGQVRVITQEGNLNIRKGPGTDQPIVGKAA
                     KGEIITLISKSTDQWWLVKTNDGEQGYSYAAYLEPVL"
     gene            complement(3976..4458)
                     /locus_tag="PLN75_08155"
     CDS             complement(3976..4458)
                     /locus_tag="PLN75_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRG04127.1"
                     /translation="MKKTSFLSMLALTLLIAVGVTSCKPKDADILKAVQTAIATSPDA
                     AGVTAAVDKGVVTLTGEVKDEATSIALNALAAGVKNVKSVVNNLTVVPPAPIISASDQ
                     AIIDGLVAALKDNPTVTSAVKDGIITLTGEVKKADLPKILQKISTLKPVKIDNQLTVK
                     "
BASE COUNT         1397 a          960 c          983 g         1398 t
ORIGIN      
        1 gtgagagtcg tataatttat aaaaaaatct aaactttagg agataattac agctatggaa
       61 ttaaataatt atagatttgt ataaaaattt tatagtaatg agaaacagtt tttatatttc
      121 ttttctgatt gtcctgctga ttctaacata tagtccgctt tatgcagtac ctgcctatcc
      181 attccctcag caaattttgc aacccgatgg tacatcactc tccgtaattt tgaaaggtga
      241 tgaatttcat cattatcacg tcaccgaaga tgggtatttt ataaagagag gtatcaacgg
      301 ggtgtttaac tacgccatac ccgacgaata tggccggttg caagacacga aaatcaaggc
      361 aaacgatcca aataaaagag aaagcgccga aaaatcgttt gtcagcgcat tgcaggtgaa
      421 ccaatcattg cagcgcatca ataaagaagc aagagttgaa agaactcctt catcaaccaa
      481 tgccggacaa aaaagtcaat ttccccgcgt aggttcaccc aaatcactgg tcattctggt
      541 caatttcaag gatacttctt tcgttacgcc caatccgaaa atggcatttt cagccttgct
      601 caacgagaaa ggatatagcg caaacggcgg taccggatca gcccgcgatt attttataga
      661 taattcttcc ggaaaattca gccccgaatt tgatgtggta ggacccttta ccctgcccca
      721 gcgtatgtct ttttacggtg aaaacggaac aaacgattct gataaaaatc cggtacaaat
      781 gatagtggat gcctgtaagg cggctgatga tgccggagta gatttctcgg tttacgatac
      841 tgaaaacgat ggaatcgttg ataacgtatt tgtatattac gccggataca acgaagcaga
      901 gtggggtggt gtaaatacca tctggcctca ccggtggggc atttatccca cgagtatata
      961 caacggcgga aactacggtg gttctgcggc atcggtaaca ttcgacggga aacgggtgga
     1021 agactacgcc tgcacctccg aattaagagg gaaaaccgga acaaacatgg caggcatagg
     1081 caccttcacg catgaattcg gacatgtaat cggcctggcc gatatgtatg ccaccaacgg
     1141 agccaagcat cacacattat cttactggga tataatggat gccggtgcgt accttaatca
     1201 gggaagaact cctccggctt acaacacatt cgagcgtatg cagctgggtt ttatcacacc
     1261 ttatatactt acaggtcccg aggatgtgaa actacaaccc ttaacgacac acaaccaggc
     1321 atacctgatt tctccgagtg atgtacacaa cttaaatgga aataatccgt caccggtaga
     1381 atactttctg ctcgaaaacc ggcagaaagt tgggtgggat gcttatttgc cgggtcacgg
     1441 gatgcttata tacagaattt attacaatca aaacgactgg tactacaacc aacccaacaa
     1501 cgatccgctg aaaatgggag tggacattat tgaagcagat aaaacagcga atacaacctc
     1561 gtttgcagga gatccatttc ccggtacttc aaatgtagat tcatatattc cggctcttcg
     1621 ttcgggaact gaactgaata agccgataac atcaataata gaatcagata aaaccattat
     1681 tttcagatac aaaggcggga aaaatgtacc agtgttgagt gctgaaagta atttcactcc
     1741 tgcgtcaacg gttcagggca ctccgtccga atcacaatca ataaccgtcg caggaatcag
     1801 actcgtgtct gatatgatta tttcatttgt gcagaatgaa cattttgaag taaaaaaaga
     1861 aactgatccc gaaagcgcct gggctaaatc actcaaactg actcctgttg attcggtggt
     1921 aactcccatt aagatactgg taagatataa tcccaccgta ccttcatacc gcattatgca
     1981 cagcgatatg atgaatatta catcggttta tgctgctacg ataagcaaaa atgtaaccgg
     2041 aaaatcgaca cggccggtat atgtggttga acctacggca acgaaagaat ccgaccttac
     2101 ctacaaaggg ttcgtagcca actggaatcc ggtgttcgat gctaccggat attatctgac
     2161 ggtaattgaa acgaatgcag ccggagaaca atcacccata tttactgata aatgggtgga
     2221 aactaccagc tatacgctgt atcatctgat ttctggcagg aattattttt acaaagtgaa
     2281 agccagcgat aaaaatttac agttcgaata tgaaaatatc accggatatt ccaataccgt
     2341 gcaggtaaaa accccggatt atccttatga gaaagaactt cgtgcagtgg taagcaaagg
     2401 ttcggtaatg gtatttgtgc catctgacga caaaagcgca ggagagccgg gtgctatcaa
     2461 aaccgtggat atcttcaaca cgatgggtca aaaagtgaaa agctatccgg ctgacaggga
     2521 catagtggag atttcggatt taccccgcgg aatggttttt attattcagt cgggtaaata
     2581 ccgtacgaaa gtaattctgt aaaataaatg ttgtgagatt agcatctaca taacttctgt
     2641 tagacttcat aaagccattc atgaaatcag attgctgtaa atttccattt tgataattga
     2701 gtctgatccg aaaaccttgt ttttgccaca aagacactaa aacaccaagt tacacaaaga
     2761 aactaatttt atatctaatt gattatcaac atatctaatt tcgacacaaa ggataaaaat
     2821 cttagatttt tataaattat tacttttcgg agtggactca taattcgata aaaaataaaa
     2881 agagctgtcc caaatttgga ttgagacagc tcattttttt gttgtcattt aacgtgagtt
     2941 cggaattgat cttcccttat aaaaagcctt tctcgctctt gtgtcacatc aattgataaa
     3001 tccccgtaga aattacagcg tttgatcgtc cagatctgca tcgggtgcgt ttttctttac
     3061 cgattctatt ccgttgtcgc gggcggcttc gctttcgtac atttcgctgt taccgattat
     3121 ttgtccgttg gtggcagtga ggttgaaaaa tggtttgccg ttgcttgaca ctttccggtc
     3181 gaagcgtgca tcatcctgcg agtttttacg tacggattca atgccgttga ggcagcttgg
     3241 tttggctgca tatccctgac tgcttagaat tatctgaccg tttccggctt tcagactgaa
     3301 ctgaaagtca ccgtttgttc tttgtgttac tacaaatttt cccatgttta tcaagttaga
     3361 agttagaaat tagaagttag aagcttgtct gcctccggca gattagaaat taaaagaata
     3421 ataagagatg aaattaaaaa ataaaaaagg aagttgaaat taagtgaaac ttgtatcaaa
     3481 tttctgaagt tagtagtctg aaacgcccct ttccctattt tctctcttat tccccttttt
     3541 gggcgtttat aataccggtt cgagataagc agcgtatgaa tatccctgtt cgccgtcgtt
     3601 ggttttcacc agccaccact gatcggtgga tttgctgata agagtgatga tttcgccttt
     3661 ggcagctttc cctacgatgg gttgatctgt tccgggtcct ttgcgtatat tcaggttacc
     3721 ttcctgggtg atgacgcgta cctggccgcc ttctactaca gcagcgttaa cgttcataac
     3781 gacgtcgccg gctgcaaaat tcgggtcaat cgtaccgtaa acatcccaaa ggtggtcctt
     3841 ggcagcaccg cttggcacat cgccgttgat atacaacaca ccatcctgtt caactacctg
     3901 caggtttgta gctccggatt gattggcagc atcgatgaga gctgcatatt tttctttcag
     3961 tgtcatggat tttacttatt ttacagttag ttggttgtcg atttttaccg gtttgagagt
     4021 cgagattttt tgaagaattt taggcaggtc ggcctttttt acttcacctg tcaatgtaat
     4081 aattccgtcc tttacagcac tggtgaccgt gggattgtct ttcagggctg ctaccagacc
     4141 gtcgataatt gcctgatccg atgcgctgat aatgggtgca ggcggaacca ccgtcaggtt
     4201 gttaacaaca gatttcacgt tttttactcc cgcagcaagt gcgtttaatg caatgcttgt
     4261 ggcttcgtct tttacttcgc ccgtcagggt taccacacct ttatcaactg cagcggttac
     4321 tcctgcagca tcgggactgg tagcaatggc tgtctgaaca gctttcagga tgtctgcatc
     4381 ttttggttta cacgatgtta caccaactgc tatcagcagt gtaagtgcaa gcatggaaag
     4441 gaaacttgtt tttttcatgt tttttgtttt ttaaaagtta gtgaattaaa ttaatagtta
     4501 aataacgatt acaagtgtta ttttgttttg aaagaacatt ttgaaagaac aaaatctgtt
     4561 atttttttat ccggaataat tttactgcaa gaactgtgct aaaattacaa aaatttgtaa
     4621 atgaacagtt tttgttttaa gtattttttt tgtttttatt ctcctcaata actttcaata
     4681 aatcagttga atttgaataa tttgtgtaat aaaattaaaa tatttctcat taaataga
//
LOCUS       DAORKP010000159         4564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4635_length_4564_cov_9.995343, whole genome shotgun sequence.
ACCESSION   DAORKP010000159 DAORKP010000000
VERSION     DAORKP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_4564_cov_9.995343"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..886
                     /locus_tag="PLN75_08160"
     CDS             128..886
                     /locus_tag="PLN75_08160"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRG04128.1"
                     /translation="MKIKKILVSQPKPVTEKSPYFDIIEKYNVKIDFRPFIKVEPIPA
                     KEFRTQRINILEHTAIIFNARHGIDHFFHLCEELRVTIPESMKYFCVSETVALYLQKY
                     IQYRKRKIFFTQTGKFADLAAILNKHTDEKYLYITSDIRNEETMSVLENAKINFNQAI
                     MYRTVSTKISEEELQDYDMLVFFSPAGIKSLLENAPNMQQGDRVIASYGSTTAQAVHE
                     AGLRLDLEAPSPGVPSMTMALENFLKEQKKNGKK"
     gene            975..1385
                     /gene="rnpA"
                     /locus_tag="PLN75_08165"
     CDS             975..1385
                     /gene="rnpA"
                     /locus_tag="PLN75_08165"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRG04129.1"
                     /translation="MNTFPKTEHLFGEKVIENLFKNGQSFITYPYRVVFLDVSHSVEE
                     LPLRVMMSVSKKKFKKAVTRNRIKRLMREVYRLNKTELVNFASDEKIKIHVAFQYVAD
                     EILSFDEMNNKMIKVTDKLKRNLLENLKKTDENG"
     gene            1372..1602
                     /gene="yidD"
                     /locus_tag="PLN75_08170"
     CDS             1372..1602
                     /gene="yidD"
                     /locus_tag="PLN75_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRG04130.1"
                     /translation="MKTAKKVIEFIFLLPVYFYRIFISPMTPPSCRYTPTCSAYAIEA
                     VKKHGILKGGWLALKRILSCNPWGGSGYDPVP"
     gene            1734..2414
                     /locus_tag="PLN75_08175"
     CDS             1734..2414
                     /locus_tag="PLN75_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRG04131.1"
                     /translation="MKKQVSVAYLISGLLFSTCLIVANIVEQKLIQIGPIEATAGLLI
                     FPISYIVNDIIAEVWGYRKARLIIWYGFLMNFLAVAVFRLSILVPGSENFTHQEAFSL
                     VMGNTLRITVASFIAFLIGSFLNAFVMSKMKIMQKGRNFSLRAVVSTLIGEGADSIVF
                     FSIAFMGVIPNDDLWILMLTQTGMKTAYEIIILPFTNRIVTWVKKKEEEDVFDENVSY
                     NPFRIKDI"
     gene            complement(2430..3200)
                     /locus_tag="PLN75_08180"
     CDS             complement(2430..3200)
                     /locus_tag="PLN75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRG04132.1"
                     /translation="MKQRILYLSVLISFTLQTYAFDAVGHRIIADIAYENLKCSTRKK
                     LDKMLGKHGMIYASVWSDDIRSDSAYAFSYNWHFQNLKDGMTKPELQFLLDNPSSEGK
                     HLFLALDSLSKNLKQNKNDVEALKFIVHFSADLFQPMHLGRLSDRGGNDVMIKWFGRD
                     IRLHQLWDTQMLEGQKFSYSEYSSYLKDKFAKQKKELKKQSKPDAIWETYLLRNKIYG
                     YDYENLRAYNYLYEFNEDMDRQLFRAGIQLANLLNQIF"
     gene            3276..4202
                     /gene="xerD"
                     /locus_tag="PLN75_08185"
     CDS             3276..4202
                     /gene="xerD"
                     /locus_tag="PLN75_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRG04133.1"
                     /translation="MLSLLQEYHVYLKMEKSLSKNSVDAYERDIKKLISYLNEAHIKP
                     EEAKLEHLLDFIVEIGETGIDKRSQARLISGIRSFYRFLIYKDKISQDPTELLEAPKI
                     GLRLPEVLTVNEIDRIFAAVDLSKPEGQRNRAIIEVLYGSGLRVSELINLKLSNIYFD
                     ENYMLVEGKGSKQRLVPLSEESVKQIGFWRLDRNKLDIKKGNEDYLFLNRLGSRLTRA
                     MIFTIVKDLCVVAGIKKNVSPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYT
                     HIDVQYLRETILMYHPANRARKRQNNDKQQIN"
     gene            4324..>4564
                     /locus_tag="PLN75_08190"
     CDS             4324..>4564
                     /locus_tag="PLN75_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG04134.1"
                     /translation="MLLFFVQSLSAQETLVVGQVFNKYDRTPLESVSIFFKGSNVHTQ
                     TNEEGYFLIRNQGDESVLVFSLIGYVKEEIKVKPGE"
BASE COUNT         1438 a          876 c          893 g         1357 t
ORIGIN      
        1 attcaagaat attagattta ttctatattt ttttgtacct ttgcacgctc gaaattatac
       61 ccttattgtt gttattctgg gcatataatc tagctgttta atgttttaac taaaaaacca
      121 taaagtcttg aaaatcaaaa agattctggt ttcacaaccg aagcctgtaa ccgaaaaatc
      181 accgtatttt gatattattg aaaaatataa tgttaaaatc gattttcgcc cttttattaa
      241 ggtcgaaccc attccagcaa aagaattccg gactcagcgt atcaatatct tagagcacac
      301 agctataata ttcaatgcgc gtcacggtat tgatcatttc tttcatctgt gtgaagaact
      361 cagggttacc attcccgaat ccatgaagta tttttgtgta tctgaaacag ttgcactgta
      421 cctgcaaaaa tacattcagt accgtaaaag aaagattttt ttcactcaga ccggaaaatt
      481 tgccgattta gctgctattc tcaacaagca taccgacgaa aaatatttat acatcacttc
      541 cgatatccgt aatgaagaaa caatgtctgt tttggagaat gccaaaatca atttcaatca
      601 ggcaatcatg tacagaacgg tgagcaccaa aatttcggaa gaagaacttc aagattatga
      661 tatgctggtg tttttcagcc ctgcaggtat caaatcactg cttgaaaatg ccccaaatat
      721 gcaacaggga gacagagtca ttgccagcta cggctctact acagctcagg cagtacacga
      781 agcaggtttg agacttgacc tggaagctcc atcgcccggt gtaccttcca tgacaatggc
      841 tctggaaaat tttcttaaag agcaaaaaaa gaacggtaaa aaataaggca tcaacttatc
      901 gaaataatta acattcggat atcccgacag tactgaaaat ttcgtacttt tgggatattc
      961 tttttcataa aaaaatgaat acatttccga aaacggaaca tctctttggt gaaaaagtga
     1021 tagaaaacct gttcaagaat gggcaaagct tcattacata tccatatcgg gtggtttttc
     1081 tggatgtaag tcattcagtc gaagagcttc cgctgagggt aatgatgagt gtttcgaaaa
     1141 agaaattcaa aaaagcagtg acccgcaacc ggattaaaag attgatgcgt gaagtttaca
     1201 gactcaacaa aacagaactg gtaaactttg catcggatga aaaaattaaa atacacgtgg
     1261 cttttcagta tgttgccgac gagattttaa gcttcgatga gatgaataat aaaatgatta
     1321 aggtaactga taaactgaaa agaaatttac tcgaaaatct gaaaaaaacc gatgaaaacg
     1381 gctaaaaaag tcatagaatt tattttttta ctaccggtgt atttctatcg tattttcatc
     1441 tcgcctatga ctccgccttc atgcaggtac acaccaacct gctcggctta tgcgatagaa
     1501 gcagtaaaaa aacacggaat tctgaaaggc ggctggctgg ctttgaaacg aattctcagt
     1561 tgtaatccat ggggcggcag tggttatgac cccgtaccat aacacccaaa actcttatta
     1621 tggcagtaat cggtttacgc acaaaagtga ttaatttatt catatttgca aaaaacaatt
     1681 aatagataat tccaaaccgt gtgcttgaac accacgtaaa aaaaacaaga agtatgaaaa
     1741 aacaagtttc agtagcctat ctgataagtg ggctgctatt ttccacctgc cttattgtag
     1801 ccaacatagt tgagcaaaag ttaatccaaa tcggtccgat tgaagccact gccggattac
     1861 tcatttttcc gatttcctac attgtcaacg atattatcgc tgaagtatgg ggctacagaa
     1921 aagcacgact gataatctgg tacggttttt tgatgaattt tctggctgta gcagttttca
     1981 gactgagtat actggtgccg ggttctgaga attttaccca tcaggaagcg ttttcgcttg
     2041 taatgggcaa cacactcagg ataactgttg caagtttcat tgcatttctg attggttctt
     2101 ttctcaatgc tttcgtgatg agcaaaatga aaattatgca aaaagggcgt aatttttcgt
     2161 taagggctgt agtttcaact cttatcggag aaggggccga ttccatcgta ttttttagca
     2221 tcgcattcat gggtgtcatt ccaaacgacg acttatggat attgatgctc actcaaaccg
     2281 gtatgaagac tgcttacgaa atcatcattc ttccgtttac caaccgtatt gtgacgtggg
     2341 taaaaaagaa agaagaggaa gatgtttttg atgaaaatgt gtcctacaat ccattcagga
     2401 taaaggacat ttaaaaaatg gttaagtggt cagaaaattt gatttaaaag atttgccagt
     2461 tggattccgg ctctgaaaag ctgccggtcc atatcttcat tgaattcgta taaatagttg
     2521 taagccctca gattttcgta atcataccca taaatcttgt ttcggagcag gtacgtttcc
     2581 cagattgcat cgggtttcga ttgtttcttg agttcttttt tctgctttgc aaatttatct
     2641 ttcagatagc tgctgtattc ggaataagaa aacttctgac cttcaagcat ctgcgtatcc
     2701 caaagctgat gaagccttat atcacggccg aaccacttga tcatcacatc gttgccgccc
     2761 cggtcggata aacgcccaag gtgcatcggc tgaaataaat cggcagaaaa atgaacgata
     2821 aatttgagtg cttccacatc attcttattt tgcttcagat ttttcgaaag gctatcaagt
     2881 gcaagaaaca ggtgttttcc ttctgaggat ggattgtcga gcagaaactg cagttccggt
     2941 ttggtcatcc cgtctttcag attctgaaaa tgccagttgt agctgaatgc ataagcagaa
     3001 tcacttctga tatcgtcgct ccacacggaa gcatatatca ttccgtgttt tccaagcatt
     3061 ttatctaatt tttttcgggt actgcatttc agattttcgt aagctatatc cgcaataatg
     3121 cggtgtccga ctgcatcaaa ggcataagtt tgcagtgtaa aactgattaa tacggaaaga
     3181 tagagaattc tttgtttcat tagtgttatt ttaaatgaaa acaaagatag aaaatatttt
     3241 tcataccttt gcagaatcgg ttaatttagt catccatgct ttcgctcctt caagaatatc
     3301 atgtttacct gaaaatggaa aaatcgttgt cgaaaaattc ggtcgacgct tatgaacgtg
     3361 atattaaaaa attgatttca tatctgaatg aagcgcacat aaaacccgag gaagccaaac
     3421 tggagcatct actggatttc atcgttgaaa tcggcgaaac tggaattgac aaacgatcac
     3481 aagcgaggct tatttcgggt atcaggtcgt tctaccgttt ccttatttac aaagataaga
     3541 tttcgcagga tcctaccgaa ttgctcgaag cacccaaaat tggtttacgc ttgcctgagg
     3601 tacttacggt gaatgagatt gaccggatat ttgcagctgt tgatctttca aaacccgaag
     3661 gacaaagaaa ccgcgcaatc attgaggtgc tttatggttc gggtttgcgg gtttcagaac
     3721 tgattaatct gaaactttcg aacatctatt ttgatgaaaa ctatatgctt gttgaaggca
     3781 aggggagtaa gcagcgactt gttccacttt cggaagagtc agtgaaacaa atcggttttt
     3841 ggcggctgga ccgcaacaaa ctcgacatca aaaagggaaa tgaagattat ctttttctga
     3901 accggcttgg aagcagactt acccgggcta tgatttttac gatagtcaaa gacctttgcg
     3961 tcgttgcagg aataaagaaa aacgttagtc cgcatacatt tcgtcattca ttcgccacac
     4021 atctgctcga aaatggcgcc aatttgcgtg ccattcagca gctactcggt catgaatcta
     4081 tcacaaccac cgagctttat acacatattg acgttcaata tctgcgagaa acaattttaa
     4141 tgtatcatcc ggcaaacaga gctcgtaaaa gacaaaacaa tgataaacag caaattaact
     4201 gagttactta ccttaaaaat caaaataatg aattcatttg cgcatttctt atctttcttt
     4261 ttccggggat cgaatcggct gtcaatcatt tttttaaagc aaacaaaagc gttattcgtc
     4321 gttatgcttt tgttttttgt acaaagtctc tcagcacagg aaacattagt ggtaggccag
     4381 gttttcaata agtatgaccg aacgccgtta gagtcagtca gcattttctt caagggttcc
     4441 aacgtacata ctcaaacgaa cgaagagggt tatttcctga ttcgcaacca aggcgatgag
     4501 tctgtactgg tattctcact tattggctat gtaaaagagg aaattaaagt gaaacccgga
     4561 gaaa
//
LOCUS       DAORKP010000160         4554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4651_length_4554_cov_7.197599, whole genome shotgun sequence.
ACCESSION   DAORKP010000160 DAORKP010000000
VERSION     DAORKP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4651_length_4554_cov_7.197599"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1439)
                     /locus_tag="PLN75_08195"
     CDS             complement(210..1439)
                     /locus_tag="PLN75_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04135.1"
                     /translation="MKNEKHIAELTSRLRAKYGKPVDSVVSIANLAEEVGTSEQTMRR
                     FLGKVKTKNGINPVSDKSLSVICRYVGYRDWSHFTQSNGNGHGHPEPDKLYIEHMEPF
                     FRNGKKYNLNYQQDTITVDMLNDYIKVIYATKQNIAHFYNLNHENNWAADYVLAWLPN
                     YNYFGQNWFRAILSKHAAHTDKPLVRLALTNFLRFGAFLTNDNVAYTPDSETLNRAYA
                     AYRKNNEYMPFQEMRYYTNLLIDAKRNNETQKFYGLLHQFLQEFEEQNIREFNKHEVM
                     IFFCNTLIWLKEYQVAYELLKNRTGFITHFAANSAKERALHFYGINLAFMKTTFALTW
                     AVNRIPEPRLLALTSADFDHPTGLLYNDYIHVMYQARCILTGRGAKHKKTLYDELKTL
                     TDKTNYVRIYDLVGKAT"
     assembly_gap    1676..1775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2535..3788
                     /locus_tag="PLN75_08200"
     CDS             2535..3788
                     /locus_tag="PLN75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRG04136.1"
                     /translation="MLNDYSIQISVLIIVLACVLFFSLYKSNKNILFLSSFLIIYAIQ
                     SVTVGLVFYGGPVWLLAILFIHFNPLYYLAPALFYFSVRASLTDSMHLSKRDLLHLIP
                     FLVMLVAAIPYYFTGFSYKLELASGLMGNYDAYLDYGKVVVFNEHFNQFVRPVIFLGY
                     MVASVVQAHRFLPKYRSAYGPVLKQYRFQLRSIYLAIVVFSIHAIVQLIVTIDYLFEP
                     VIIQGLDHISFLLHVSGIMYLCIPFYIITQPRFLYGMPQLDNSLHTANDDSHRLTDGN
                     GHHSDKQPKENHFSENENLIRLSEQIMAYLHAEKPYLNEKFSVLDICERLSVPRHHVQ
                     YCFNMIMNKSFSEMKNELRIQHAMELMKINSKNNLSIEGIGRQSGFASNSSFYTSFKE
                     ETGLTPSKWMELHAAASTETVTRQS"
     gene            4010..>4554
                     /locus_tag="PLN75_08205"
     CDS             4010..>4554
                     /locus_tag="PLN75_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04137.1"
                     /translation="MEELKQFGVIPVDVAALETVFSSYSFPQKKITTLEQTGKLIRLK
                     RGLYVVAPGVSGRILSEELIANHLYGASYVSMETALRYYGLIPENVYTVTSMTLKLTK
                     YYENSVGKFEYIHCAKDYYSIGIRQVTHEDYAFQLASPEKALCDLIAYTPKLRLRTVK
                     SVRVFLEEDLRFDMDAFYNLDA"
BASE COUNT         1156 a          938 c          956 g         1404 t
ORIGIN      
        1 cagcgaagct aattccgatt ctgtcggaat tatttggatg ctgtgagttt attgacagag
       61 ttggaactct gtccacccgt gtcactgctg aaaagagaat ccctactgta ccaaaaaata
      121 ccaggaaaat aatcttatgt tgcattctta aagtccccca agggggattt agggggcttt
      181 cctcacccca aaagtccccc aagggggatt taggtagcct ttcccaccaa atcataaatc
      241 cttacgtagt tggttttgtc ggtgagagtt ttgagttcat cgtagagtgt tttcttatgt
      301 tttgctccgc gtccggtgag tatgcagcgt gcctggtaca tcacgtggat gtagtcgttg
      361 tagagcagcc ccgtaggatg gtcgaaatcg gcagatgtca gtgccagcag tcgcggttcg
      421 ggtattctgt tcacagccca ggtgagtgca aaggtggttt tcataaaagc caggttgatg
      481 ccatagaagt gtagcgccct ttctttggcg ctgttggcgg caaagtgggt gataaatccg
      541 gtgcggtttt tcagcagttc gtaagctacc tggtactcct tgagccagat aagggtgttg
      601 cagaagaaga tcatcacttc gtgcttgttg aattcacgga tgttctgctc ttcgaattcc
      661 tgcagaaact ggtggagcag cccgtagaat ttctgagttt cgttgtttct ttttgcatct
      721 atcagcaggt tggtgtagta cctcatctcc tggaatggca tgtattcgtt gtttttccgg
      781 taggcggcat atgcgcggtt cagcgtttcg gagtcggggg tgtatgctac gttgtcgttg
      841 gtaaggaaag cgccaaagcg gagaaaattg gtgagtgcaa gacggacgag cggtttatcg
      901 gtatgtgcag cgtgtttgct gagtatagcc cgaaaccagt tttgcccgaa ataattataa
      961 ttgggcagcc acgccagtac atagtcggct gcccaattgt tttcatgatt aaggttgtag
     1021 aaatgcgcta tgttttgttt cgtggcgtag attactttta tgtaatcgtt cagcatatct
     1081 actgtgatgg tatcttgttg ataattaagg ttgtatttct ttccgtttct gaaaaacggt
     1141 tccatgtgtt cgatgtagag tttatcgggc tccgggtgcc cgtggccatt accgttcgat
     1201 tgggtaaagt ggctccagtc tctgtatccc acgtagcggc aaatcaccga aagcgattta
     1261 tcactcaccg ggttgatgcc gtttttggtt ttcactttac ccagaaaacg tctcatggtt
     1321 tgttcgctcg tgcccacctc ttcggcaagg tttgcaatgc tgaccaccga atcgaccggc
     1381 ttgccgtatt ttgcccggag tcgggaagtt aattccgcaa tgtgtttttc atttttcatg
     1441 taaaagaaac agatttaaag gtcgaataaa aaatactccg ctgtgtactt ttaaataaaa
     1501 aagttttcaa aaaaaaacag gtaaatctct tgctgtgttt tattttggca tttttagccg
     1561 tgccaaaagt aaagtttttt ttcgctcctg tcaatagttt tttcggctgc tgcacgaagt
     1621 gcccaaaata tgtaacgaag tcagttcaca gaatcagtca accggtagtt ttttgnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccca aaatatgtaa cgaagtcagt
     1801 tcacagaatc agtcaaccgg tagttttttg aacagaagaa agcaaaaaac cacagtccta
     1861 cactcccatg caggtcaccc gacgttttcg ggtgttatta aaatgattac aaacgtagtg
     1921 tatttatttc gtaaaatctc aatgaaaaat cgtgtttctt atactggttt taaaatcagg
     1981 aaagatttta ccggtttttc agcattgccc cggatcattc tgcgctgcct gatgatgcac
     2041 gctgcagtaa aataaagcat gtcgggcagg tttatcaacc gattgataat cagtgaaaat
     2101 actgtgattt ttagtgcagg ttttttactg tcaggatgcc atttcgggag ttaaattgaa
     2161 aaaataatat cgcaccgttt gtgcaactta ctatcaccgc cagccgatgc attccttcgg
     2221 gtcaaaagta ttctcaaatc gtaaattttt cgttgtcaaa tcgtaaattt taccttataa
     2281 aaacatgttt taagccgttt atatccgttt tagtttttga attcgtgtta acaccggctg
     2341 cctgccggtg aaaatagatt gaaaaataat tcattttttt cgttttttat ttttacattt
     2401 gcagttgaaa aaaagcgttg tttgcttaaa ttatttttta attccggtat tatccggctg
     2461 caggttgccg ctgttaatct atttaagctg tacactcctg gaaattgaaa aacacaacta
     2521 aacctttttc tcgtatgtta aacgactatt caatacaaat ttcagtactc atcatcgtac
     2581 ttgcctgcgt tttgtttttt tctctgtaca aatccaataa aaacatcctt tttctttcct
     2641 catttctgat aatttacgcc attcagtctg ttaccgtggg cctggtattt tatggcggtc
     2701 cggtatggct gctggccatt ctgttcatcc atttcaatcc tttgtattat ttagcgccgg
     2761 cattgtttta tttctctgtc agagcatcgc ttaccgattc catgcatctc agtaaaaggg
     2821 atttgcttca cctgatacca tttctggtta tgctggtggc tgccattcct tactatttta
     2881 ccggcttcag ctacaaactt gaattagctt cgggactcat gggcaattac gatgcttacc
     2941 tggattacgg caaagtggta gttttcaacg aacatttcaa tcagttcgtc cgtccggtta
     3001 ttttcctcgg ttacatggtc gcttcggtgg ttcaggctca ccggtttctg cccaaatacc
     3061 gctcagcata cggtccggtg ctcaaacaat accggtttca gctccgctcc atctacctgg
     3121 ccatcgtggt attttccata cacgccatcg ttcagctgat cgtgactatt gattatcttt
     3181 tcgagcctgt gataattcag ggtcttgatc atatcagctt cttattgcac gtatccggta
     3241 tcatgtatct ttgtattcct ttttatatca tcactcaacc acgctttctg tacggaatgc
     3301 ctcagctgga taacagtctt catactgcta atgatgactc acaccgactt accgacggta
     3361 acggtcatca ctccgacaag caaccaaagg aaaatcattt ttcggaaaac gaaaacttaa
     3421 tccggctttc ggagcaaatc atggcttacc tgcatgccga aaaaccctac ctgaacgaaa
     3481 aattctcggt tctggatatt tgcgagcggt tgagtgttcc acgccaccac gtgcagtact
     3541 gcttcaacat gatcatgaac aaaagtttca gcgaaatgaa aaacgagctg cgcatccaac
     3601 atgccatgga actgatgaaa atcaattcga aaaacaatct ttccatcgaa ggcatcggca
     3661 ggcagtcggg ctttgcctcc aactccagtt tttacaccag tttcaaggaa gaaaccggcc
     3721 tcactcccag caagtggatg gagttacacg cggcagcatc caccgaaacg gtcaccaggc
     3781 aatcctgatg gtattgttga attctccagt atgactccgt tttccggcaa aagcacatgg
     3841 ccgttccaac ggcattgtgc gtaaattgag aataatacct ttatttcagc agaaaacctg
     3901 acaagtattc cacacctgtc aggtttttat ctgttaatta tgtcattttt atttacagaa
     3961 aacgaataaa taatgcgtta tctatcaaaa aaaagactat ttttgcatca tggaagaatt
     4021 aaagcaattt ggagttattc ccgtagatgt tgccgcattg gaaactgtat tcagttctta
     4081 cagctttccg caaaaaaaga ttacaactct tgaacaaacg ggtaaattaa tacgacttaa
     4141 gcgtggtctg tatgtagtag cgcctggtgt atcgggcaga atattgtcag aagaattaat
     4201 agccaatcac ctgtatggtg cttcgtatgt gtctatggaa acggcacttc ggtattacgg
     4261 actgataccg gaaaacgtat atactgtaac atcgatgacc cttaaactga cgaaatatta
     4321 cgaaaacagt gttggaaaat ttgaatatat tcattgcgct aaggactact attctattgg
     4381 tatccggcag gttacgcatg aagattatgc atttcagtta gcttctcccg aaaaggctct
     4441 gtgcgatttg attgcttata ctcccaaatt gagacttcgg accgtgaaat cagtccgggt
     4501 ttttttggag gaagatttgc gtttcgatat ggatgcattt tacaacctgg atgc
//
LOCUS       DAORKP010000161         4522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4715_length_4522_cov_9.606671, whole genome shotgun sequence.
ACCESSION   DAORKP010000161 DAORKP010000000
VERSION     DAORKP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4715_length_4522_cov_9.606671"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /gene="serC"
                     /locus_tag="PLN75_08210"
     CDS             complement(<1..1056)
                     /gene="serC"
                     /locus_tag="PLN75_08210"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRG04138.1"
                     /translation="MKKYNFNAGPCVLPKPAIESAIEAIRNFDNTGIGILEISHRTPG
                     WERIMAETEQLWRDLLNIPEEYAVLFLGGGASTQFFYVPANLLGKKAAYLQTGVWAKK
                     AIKEAKFYGEVEVVASSEDKNYTYIPKGYTIPTDADYFHITTNNTIYGTEIHEDINSP
                     VPLVADMSSDIMSRPVDVSKYALIYGGAQKNVGPAGVAFVIVRKDLLGKMDRKLQTMV
                     DYRTHIGETPKDNSMFNTPPVFSIFVMHETLKWVKELGGLDAMHKINLQKANLLYDEI
                     DRNRMFVGTAVKEDRSIMNVCFVMAEAYKDKEADFMNFAKERGMVGIKGHRSVGGFRA
                     SIYNACPLESVEALVACM"
     gene            complement(1247..2647)
                     /locus_tag="PLN75_08215"
     CDS             complement(1247..2647)
                     /locus_tag="PLN75_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRG04139.1"
                     /translation="MKKQIIFIFALFVSAALFSQSPVGSWNGLLKVQGQQIRLVINIQ
                     QAENGYKATMDSPDQGAKGIPVDRVTFVNDTLKFEVKMIGVTYTGVLGSDNVIKGTFT
                     QMGMSLPLDLSAQTVEKEKIVRPQEPQKPYPYYTEEVKFTNHGGDILAGTLTLPKKTG
                     KYPVVVLITGSGPQNRDEELMGHKPFLVIADYLTRNGIGVLRYDDRGTAQSTGDFQKA
                     TSVDLANDAEAAVRYLLTRKEINKKKIGLSGHSEGGIIAPMVAARNKKVAFIVMLAGP
                     GLRGKELLELQTAAIAKASGMTETELANVLKLNSGAYNLVLHSNDTVKLKTDLTDYFR
                     KNLKDNPTPGENPTQEQLDMQAKGYTNQLASPWMQYFIKYDPFPTLRKVKCPTLALNG
                     SKDLQVPPAENLALIRKAFTDSGNKKLTAIELPGLNHLFQESTTGSPAEYAKIEQTFS
                     PKALDEILKFIRQQVK"
     gene            complement(2662..3234)
                     /locus_tag="PLN75_08220"
     CDS             complement(2662..3234)
                     /locus_tag="PLN75_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04140.1"
                     /translation="MKNKNALRYIYNPFEKIAGWQALIYGLIIACLSGWIGKQAGLMF
                     DGAIDAHFGNEVSYYQAFMMLFIDIACVGAVMYLAGLLVTRNFRFIDLLGTMTLARAP
                     YLLLSLTGLFVTIPETAELLANPALIFSNPGFLIFIALSFPAIIWVIALMYNAWKVST
                     GAKGSKMIIAFIAGLFVAEVISKILISILI"
     gene            complement(3224..3487)
                     /locus_tag="PLN75_08225"
     CDS             complement(3224..3487)
                     /locus_tag="PLN75_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HRG04141.1"
                     /translation="MLPVYCPSCHAQLKVKCLKCESCQTEVNGSYDLPLLALLSLDDQ
                     QFILQFVKNSGSLKNMANELKLSYPTVRNLLNEIILTIEKYEK"
     gene            complement(3689..>4522)
                     /locus_tag="PLN75_08230"
     CDS             complement(3689..>4522)
                     /locus_tag="PLN75_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRG04142.1"
                     /translation="GESYVANDVFLDGKTQQIIVVTGPNMSGKSALLRQTALITLMAQ
                     IGCFVPVESAKIGVVDKIFTRVGASDNISQGESTFMVEMNEAASILNNLSDRSLVLFD
                     ELGRGTSTYDGISIAWAIVEYIHEHGSCKAKTLFATHYHELNEMEKTFNRIKNYNVSV
                     KEVDKKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRADQILKQLETDNRQNGDLS
                     KPVGELATQREGYQLSFFQLDDPVLEQIRDEIKGLDVNNLTPLEALNKLNEIKRIITG
                     K"
BASE COUNT         1168 a         1023 c          943 g         1388 t
ORIGIN      
        1 catgcatgcc accaatgctt ctacgctttc gagcggacag gcattgtaaa tggatgcccg
       61 gaaacctccc accgaacggt ggcctttgat acccaccatg ccccgttcct tggcaaaatt
      121 catgaagtcg gcttccttgt ccttataagc ttcagccatc acgaaacata cattcataat
      181 cgaacggtct tccttaactg ctgttccgac aaacatcctg ttacggtcaa tttcatcgta
      241 caacagattg gctttctgaa gatttatttt atgcatggca tccagtccac ccagctcttt
      301 tacccatttg agggtttcgt gcatcacgaa aatggagaac accggaggag tattaaacat
      361 ggagttatct ttgggagttt cgccgatgtg ggttctgtaa tcgaccatgg tttgaagttt
      421 ccggtccatt ttgccgagca gatccttacg cacaataacg aatgcaacac cggcaggacc
      481 cacatttttc tgagcgccac cataaatcag cgcgtatttg cttacatcaa ccgggcgcga
      541 catgatatcc gacgacatat cggccaccaa aggaaccggg ctgttgatat cttcatgaat
      601 ttccgttccg taaatggtat tgttggtggt gatgtggaaa taatcggcat ctgtgggaat
      661 ggtgtaacct tttggtatgt aggtgtaatt tttatcttcg gaagaggcaa caacttctac
      721 ttcaccgtaa aattttgctt ccttgatggc tttctttgcc catacgcctg tttgcagata
      781 agcggctttt ttgcctaaga gattagccgg tacgtagaaa aactgtgtgg acgcaccacc
      841 gccaagaaac agtacggcat actcttcggg aatgttgagc agatctctcc agagttgctc
      901 tgtttctgcc ataatgcgtt cccagcccgg tgtacggtga gatatttcaa gaataccaat
      961 tcctgtatta tcaaaattgc gaatggcttc aatagccgat tcaatagccg gtttgggcaa
     1021 aacacatggt cctgcattaa aattatactt tttcatacat attgtttttt aagaatgaaa
     1081 tttagtaatt gaagtggaca aatttacgta tttttttctg taaaatttca tcaaaaaagg
     1141 aataatttaa cttgcctacc ggtttttgta tgaacacgca tttgactttc agtattagcc
     1201 acaaaaaaag cagaaatatc aaaattttct gctttttcgt ttcattttat ttcacttgct
     1261 gcctgataaa ttttagtatt tcatccagcg cttttggtga gaaagtctgt tctattttag
     1321 catattcagc cggcgaacct gtggtacttt cctggaaaag gtggttcaaa ccgggcagtt
     1381 caatggctgt cagctttttg tttccgctat ctgtgaatgc tttgcggatg agagccaggt
     1441 tttcagcagg cggcacctgc aggtcttttg agccgttgag tgccagcgtc ggacatttca
     1501 ctttcctgag cgtggggaat ggatcgtatt ttataaaata ctgcatccag ggtgatgcaa
     1561 gctgattggt atatccctta gcctgcatat ccagttgttc ctgtgttgga ttttcgccag
     1621 gggtgggatt atctttcagg tttttccgga agtagtcagt cagatcagtt ttgagtttga
     1681 cagtatcatt cgaatgaagc accagattgt atgcacctga atttagtttc agtacgttgg
     1741 caagttcggt ttcggtcatt ccggaggctt tggcaatagc agcggtctgt aattcgagca
     1801 gttcttttcc ccgcaatccg ggaccggcaa gcatcacgat gaatgctact tttttgtttc
     1861 tcgcagccac catcggcgca ataattcctc cttcactgtg acctgacaga ccgattttct
     1921 tcttgttgat ttctttacgg gttagcagat aacggactgc ggcttcggcg tcgtttgcca
     1981 ggtcaacaga agtggctttc tggaaatcgc ctgtggattg cgccgtcccg cggtcgtcgt
     2041 agcgaagcac tccaatgccg tttctggtta agtaatcagc aatcaccagg aaaggtttat
     2101 gacccatcag ttcctcatcg cggttttgtg gtccgctgcc ggtgatgagc accactaccg
     2161 gatattttcc cgtttttttt gggagcgtga gcgttcccgc caggatatcg cctccgtgat
     2221 tagtaaattt cacttcttcg gtgtaataag gataaggttt ctgcggctcc tgcggacgta
     2281 caatcttttc tttttcaacc gtttgtgcag ataaatcgag cggcagcgac atacccatct
     2341 gggtaaatgt gcctttaata acattatcgc tgccaagaac tccggtgtag gtaacaccaa
     2401 tcattttaac ttcgaatttc agggtgtcgt ttacaaaagt cacccggtcg accggaattc
     2461 cctttgctcc ctgatcagga ctgtccatgg tggctttata gccattttca gcctgctgga
     2521 tattaatcac caggcgaatt tgctgaccct gtactttgag cagtccgttc caggagccca
     2581 ccggactttg tgaaaaaagt gctgctgaaa caaacagggc aaaaataaaa atgatttgtt
     2641 ttttcattga attcaaattt ttcagattag tattgaaatt aaaattttcg aaatgacctc
     2701 ggcaacaaac aaaccggcta taaatgcaat aatcatttta gagccttttg ctccggtgga
     2761 cactttccag gcattgtaca tcagtgcaat cacccaaatt attgccggaa acgacaatgc
     2821 gatgaaaatt aaaaacccgg gattgctgaa tatcaaagca ggattagcca gcaattcggc
     2881 tgtttcgggt atagtcacaa ataatcctgt gagcgaaagc aataaatacg gtgcacgggc
     2941 aagtgtcatc gtaccgagca aatcaataaa cctgaaattt ctggtcacaa gtaatcctgc
     3001 cagatacatc acggcaccga cacatgctat atctataaaa agcatcataa aagcctgata
     3061 atagctgact tcattaccaa aatgcgcatc aatagcgcca tcgaacatca gaccggcctg
     3121 tttaccgatc caccctgaga gacatgcaat gatcaatcca taaatgagcg cctgccaacc
     3181 ggcaattttt tcaaaaggat tgtaaatgta tcgcaatgcg ttcttatttt tcatactttt
     3241 caatggttaa gataatttcg ttcaacaaat tcctaacggt tggatagctg agcttgagtt
     3301 cgttagccat atttttcaga cttccgctgt tttttacaaa ctgaagaata aactgctgat
     3361 cgtccagcga aagcaacgcc agtaagggca aatcgtacga gccgtttacc tctgtctgac
     3421 aactttcaca tttcaaacat ttaactttga gttgcgcgtg gcaactcgga caataaactg
     3481 gtaacatgat tctatttttt tgctgcaaat atagcatgca attttcaata atccaaatta
     3541 tttttgaatt atattaatac acagtttaat tttatttatt tctatttgta ttttatcaat
     3601 taagaacgac tttattttca caaaaaaaca gcaccggaca aaatttatcc ggtgctgttc
     3661 attttttcaa ttctaattgg tttcttacct actttcctgt aattatccgc ttgatttcat
     3721 tcagtttatt cagtgcttcc aagggtgtta aattatttac atccaatcct ttgatttcgt
     3781 cgcggatctg ttcgagcacc ggatcatcga gttggaagaa actgagctga taaccttctc
     3841 gttgcgtagc aagctcgcct accggcttac tcagatcgcc gttttgccgg ttatcggttt
     3901 caagctgttt gagtatctga tctgcgcgtt tcacaatgct ctgcggcatt ccggccattt
     3961 tagccacgtg aataccgaaa ctgtgttcac tccctccacg aaccagtttg cgcaggaaaa
     4021 tgactttttt gtccacttcc ttcaccgaaa cattgtaatt ttttatccgg ttgaaggttt
     4081 tttccatttc attcagttcg tggtaatgag tggcgaaaag tgttttagcc ttgcagctgc
     4141 catgttcgtg aatgtattcc acaatggccc aggcgatgga aatgccgtcg taggtacttg
     4201 tgccgcgtcc aagctcgtcg aacagcacca gacttcggtc cgaaaggtta ttcagaatac
     4261 ttgcagcttc gttcatttcc accataaatg tactttcgcc ctgcgaaata ttgtcgctgg
     4321 cacccacgcg ggtaaaaatc ttatcgacta ccccgatttt ggcactttcc accggcacaa
     4381 aacatccaat ctgagccatg agcgttatca gtgcagtttg tcggagcaaa gcagacttac
     4441 ccgacatgtt gggacctgta acaacgatga tttgttgcgt tttcccgtcg agaaatacat
     4501 cgtttgccac atagctttcg cc
//
LOCUS       DAORKP010000162         4521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4716_length_4521_cov_9.247649, whole genome shotgun sequence.
ACCESSION   DAORKP010000162 DAORKP010000000
VERSION     DAORKP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_4521_cov_9.247649"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1389)
                     /gene="fabF"
                     /locus_tag="PLN75_08235"
     CDS             complement(127..1389)
                     /gene="fabF"
                     /locus_tag="PLN75_08235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRG04143.1"
                     /translation="MELKRVVVTGYGAVTPLGLTATETWQNVLKGVSGAAPITHFDTT
                     TFKTKFACEVKGFDPNQYFDRKEARKMDLYTQYALVAAKEALAHSGLDLEKVDKDEVG
                     VIFASGIGGIATFEQEVANYYLHQEQGPRYNPFFIPKMISDIAAGQISIQYGFRGPNY
                     STTSACASSNHGLIAAFNHIRMGHAQVMITGGAEAAITPAGVAGFNALTALSTRNDYP
                     EGASRPFSKSRDGFVMGEGAGCIILEEYEHAMARGASILCEIAGGGMSADAYHLTATH
                     PEGLGAVLVMKRALRDAGLNPEEVDYINVHGTSTPVGDIGEVKAIRELFGEHAYKLNI
                     SATKSMTGHLLGAAGAVEGIISINALINNIVPPTINFHEGDEDPEIDYNLNFTFNRAQ
                     ERKVDVVLSNTFGFGGHNACVVFKKFTP"
     gene            complement(1413..1649)
                     /locus_tag="PLN75_08240"
     CDS             complement(1413..1649)
                     /locus_tag="PLN75_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRG04144.1"
                     /translation="MSEVAEKVKAIIVDKLGVDEADVVETASFTNDLGADSLDTVELI
                     MEFEKEFGVSIPDEEAQKISTVGDAIAHVEALKK"
     gene            1802..2368
                     /locus_tag="PLN75_08245"
     CDS             1802..2368
                     /locus_tag="PLN75_08245"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRG04145.1"
                     /translation="MPKNIAIFASGSGSNAENIIRYFSRSDLFHFPVIVSNRADAFVH
                     RRAEDLNIRSVTFGRKEFETGEAVLELLREYRVDAIVLAGFLLKVPELLIDAFPERII
                     NIHPALLPKYGGKGMYGDHVHKAVRASGDTESGITIHYVNKHYDEGAVVFQATCAIEP
                     SDSYEDIARKVHALEYEHFPKVIEMIFG"
     assembly_gap    2376..2475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2658..3761
                     /gene="ychF"
                     /locus_tag="PLN75_08250"
     CDS             2658..3761
                     /gene="ychF"
                     /locus_tag="PLN75_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRG04146.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNKLAELVHPQKIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFDDDNVTHVDGSVNPVRDKEIIDAELQIKDLETIEARINKVEKQAKTGGDKEAL
                     RAFHIYSRFREALQQGLSARTVTFDNKDDEKTARELYLLTYKPVMYVCNVDEASAVSG
                     NKYVDALRESVKAENAEILIVAAKIESEIAEFDTYEERQMFLEEIGLQESGVARLIKA
                     AYHLLDLQTFLTAGPQEVRAWTFHRGWKAPQCAGVIHTDFEKGFIRAEVIKYNDYLAL
                     KSEAACKEAGKMNVEGKEYVVQDGDIMHFRFNV"
     gene            3809..4288
                     /locus_tag="PLN75_08255"
     CDS             3809..4288
                     /locus_tag="PLN75_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG04147.1"
                     /translation="MLTIRPIQSSDNEILAKIIRQCFRDFGAPTAGTVYEDPTTDDLY
                     ALFRKEKSVLWVAEWSGEVVGCCGIFPSENLPYGYVELVKLYLSADARGKGIGKALMI
                     KSIESAGELGYTNIYIESLPEFDTAVSLYEKSGFLRLNNPIGNSSHPGCNVWMVKEL"
     gene            complement(4343..>4521)
                     /locus_tag="PLN75_08260"
     CDS             complement(4343..>4521)
                     /locus_tag="PLN75_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRG04148.1"
                     /translation="IAKDQNGDRYYMVKNSWGEDSKYKGIWYVSKPFVMEKTINILVH
                     KDAVPKEIRKKLGI"
BASE COUNT         1142 a         1011 c         1046 g         1222 t
ORIGIN      
        1 ggattgaatt tcatttatat acaaaaaggc tttcaatcgt tttgaaatta acgccagata
       61 tgactgcaat cctttgacaa gactgcgtta agtcctccct gtggcctgtc cgcctctgga
      121 acggatttag ggggtgaact tcttaaacac cacgcaggcg ttgtgtccgc caaatccgaa
      181 cgtattggag agcactacat ctactttgcg ttcctgtgcc cggttgaagg tgaaattgag
      241 gttgtagtca atttcaggat cttcatctcc ttcgtggaag ttgatggtag gcggtactat
      301 attgttaatc agcgcattga tagatataat tccttcaacc gctcccgcag ctcccagcag
      361 gtgacctgtc atggatttag tggcgctgat gttcagtttg taagcatgtt caccgaatag
      421 ttcacggatg gctttcactt cgccgatgtc gccaacgggt gtggaggttc cgtgtacgtt
      481 gatgtagtcc acctcttcgg ggttcagtcc ggcatcgcgt agcgcccgtt tcattaccag
      541 cactgctccc agtccttcgg ggtgagtagc agtcaggtga taagcatcgg ccgacatacc
      601 acctcctgct atttcgcaaa ggatgctggc accgcgtgcc atggcatgct cgtattcttc
      661 caggataatg catccggcac cttctcccat cacaaatccg tcgcggcttt tgctgaatgg
      721 acgtgaagct ccttccggat aatcgtttcg ggtagaaagt gcggtaagcg cattgaatcc
      781 ggctactccg gcaggtgtaa tggctgcttc ggcacctccg gtaatcatta cctgtgcgtg
      841 tcccatccgg atgtggttga atgcagcgat caatccatga ttagacgatg cacacgccga
      901 tgtggtagag taatttggtc cgcggaatcc atactggatg gaaatttgtc cggcagcaat
      961 gtccgaaatc attttcggga taaaaaacgg attgtatcgc ggaccctgtt cctggtggag
     1021 gtaatagttg gctacttcct gctcgaaagt ggctataccg ccaatacccg aagcaaaaat
     1081 tacacccact tcatctttat ccactttttc aaggtcgaga ccgctgtgtg ccagcgcttc
     1141 tttggcagct accagtgcgt actgcgtgta aagatccatt ttacgggctt ccttgcggtc
     1201 gaaatattgg ttgggatcaa aacctttcac ctcgcaggcg aatttggttt tgaatgtggt
     1261 ggtgtcaaaa tgggtaatag gcgcagcacc actaaccccc tttagaacgt tctgccacgt
     1321 ttccgtggca gtaagtccta aaggagtaac agcaccataa ccggttacca ctactctttt
     1381 taattccata aaaaatgtct tttcagggaa atttattttt tcaaagcttc tacgtgagcg
     1441 atagcgtcgc ctacagtcga gattttttgt gcttcttcat caggaataga aacgccgaat
     1501 tctttttcga attccatgat cagttctact gtatccagcg agtcagcgcc aaggtcgttt
     1561 gtgaaactgg cagtttctac tacatctgct tcgtcaacgc ctaatttatc tacgataata
     1621 gcttttactt tttctgcaac ttctgacata atttttcttt ttaagtgagt aaataaaatt
     1681 tgtttttata aatttgcgct gcaaaggtaa ttaatttttc atgaattcaa atcacatcca
     1741 aagaaaaaag tgtaaaaatt tgcacaaatt cactcatttt gagcaataaa tccaactaca
     1801 tatgcctaaa aatatcgcta ttttcgcatc gggttcgggc agtaatgccg aaaatattat
     1861 ccgctatttc agtcgttccg acttgtttca ttttccggtt attgtatcga accgcgcaga
     1921 tgcctttgtc caccgccgcg ctgaagacct gaatatccgg tcggtcactt tcggccggaa
     1981 agagttcgaa accggcgaag ctgtgctaga actgctccgt gaataccggg tggatgctat
     2041 cgttttggcc ggctttctgc tcaaagtgcc cgaactgctc atcgatgcct ttccggagag
     2101 gattattaac attcatccgg cgctgctccc caaatacggc ggtaaaggga tgtatggcga
     2161 tcatgtacat aaggcagttc gtgcatcggg cgacaccgaa agcggaatta ccatccacta
     2221 cgtgaataaa cattacgacg agggagcggt cgtttttcag gccacgtgtg cgatagagcc
     2281 ttcagacagc tacgaagata tagcccgtaa agtgcatgcg ctggagtacg aacatttccc
     2341 gaaggtgatt gagatgatat tcggatagtt ctgagnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnngagtt ctgagttctg agttctgaga tattccaggt tgtttttggc
     2521 ttctgatttc tgatttctga tttctgattt ctaatttaaa gcagtacctt tgtgtgctga
     2581 aaaaatatta accaatctcc tcttttgtct gattatctat tgtctgtcag cgaagcggtc
     2641 taacagtctt aataaaaatg tctttacaat gcggtatagt gggtcttcca aacgttggta
     2701 aatccactct tttcaattgt ctttccaatg ccaaagccca ggcggctaat tttccgtttt
     2761 gcaccatcga acccaatgtg ggagtcatca ccgtacccga cgaacgcctg aacaaactcg
     2821 cagaactggt tcatcctcag aaaattgtgc ctaccacggt ggaaattgtg gatattgccg
     2881 gactggtaaa aggtgccagc aaaggcgaag ggctcggtaa taaatttctg gccaacatac
     2941 gcgaaacaga tgccattctg catgtgctcc gctgcttcga cgacgacaac gtaactcacg
     3001 tggatggctc ggtaaatccg gttcgcgaca aggaaatcat cgatgccgag ttacagatta
     3061 aagaccttga aaccatcgaa gctcgcataa ataaagtgga aaaacaggct aaaaccggag
     3121 gcgacaagga agccctgagg gcttttcata tctattcccg gttccgcgaa gcgctgcaac
     3181 agggactctc agcacgcaca gtgacattcg acaacaaaga cgatgaaaaa acagcccgcg
     3241 agctttacct gctcacttat aaacccgtga tgtacgtgtg caacgtggat gaagccagtg
     3301 ccgtatcggg caataaatac gtggatgcac tccgcgagtc cgtgaaagcc gaaaatgccg
     3361 aaatactgat tgtggcagcc aaaatagaat cggaaattgc cgagtttgat acttacgagg
     3421 agcgtcagat gtttctggaa gagatcggat tgcaggaatc gggtgttgcc cgcctgataa
     3481 aagcggcgta tcatttgctt gatttgcaga ctttcctcac tgccggaccg caggaagtgc
     3541 gtgcctggac attccacaga ggatggaaag ctccgcagtg tgccggagtg attcataccg
     3601 attttgaaaa aggttttatc cgggcagagg tgatcaaata caacgattat cttgctctca
     3661 aatcggaagc tgcctgcaaa gaagccggaa aaatgaatgt ggaaggcaag gaatatgtgg
     3721 tgcaggacgg cgatatcatg catttcaggt ttaatgtgta gcccctccaa acctccccga
     3781 aagggaggct ttaagaaaga gtgcagaaat gctgaccatt agacctatac aatcctccga
     3841 caatgaaata ctggcgaaaa taattcgtca gtgttttcgt gatttcgggg cacccactgc
     3901 cggaacggtg tacgaagacc ccacgaccga cgatctgtat gcccttttcc ggaaagaaaa
     3961 atcggtgctg tgggtggcgg aatggagtgg agaagtggtt ggctgctgcg ggatttttcc
     4021 atcagaaaat ttaccatacg gatatgtgga actggtgaaa ctctatctct cagccgatgc
     4081 ccgcggcaaa ggaatcggga aagcgctgat gattaaatca atcgaatctg ccggggaact
     4141 cggttataca aatatttaca ttgaaagtct gcccgagttt gatacagccg tttcgcttta
     4201 tgaaaaatcg ggtttcctcc gactgaataa tccgattgga aattcgagcc atccgggatg
     4261 caacgtgtgg atggtgaagg agttgtaaaa aaataaaaag ccggaattta tttccggctt
     4321 ttcttttctc cattcatcgg gattaaattc ccagtttctt gcgtatttct ttcggtactg
     4381 catctttgtg aaccagaatg ttgatggttt tctccatcac aaaaggtttg cttacatacc
     4441 agattccctt gtatttgctg tcttctcccc acgagttttt caccatgtaa taccggtcac
     4501 cgttttgatc tttcgcaatg c
//
LOCUS       DAORKP010000163         4513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4737_length_4513_cov_9.379767, whole genome shotgun sequence.
ACCESSION   DAORKP010000163 DAORKP010000000
VERSION     DAORKP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4737_length_4513_cov_9.379767"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1026)
                     /locus_tag="PLN75_08265"
     CDS             complement(172..1026)
                     /locus_tag="PLN75_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRG04149.1"
                     /translation="MVKSNSIVTDNSDKSTLIPPSGGGGATIWITGASSGIGEACSYL
                     FASEGAKLILTATREEQLKKVQQKCIELGAECEILPCDLLQIDRLDAIADKALSIFGK
                     IDILFLNAGISQRSKAIDTDISVDKKIMDVNFYAPVKIAKRILPEMITCGGGTIAVTT
                     SITGKFGFPLRSAYAASKFALYGFFETVQAEYYEQNIRVVMVCPGRVKTNISFNALEA
                     DGTKYAQMDDGQAGGISAEKAARKIVKAIKMQKPEVLVGGKELLMVYIKRFLPGLARK
                     MVRKVKAT"
     gene            complement(1094..2428)
                     /locus_tag="PLN75_08270"
     CDS             complement(1094..2428)
                     /locus_tag="PLN75_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRG04150.1"
                     /translation="MLRIKIQYKYELKCAECGYVTPDFWTWFGQEQKCPKCGSKHSEV
                     WYRRNYKKLLRLWKSTPNSFWHYFTFLPLLRKRHIISRNEGAVPLERWAFLENFAKER
                     YNLNIEVHVYRNDLNGGTGTFKDIAASLAASIFNEIGLKQYCIASTGNSATAYAKYLS
                     MANVNCSVFMPEDAIKTSEATISSYGQQVFRVLGDYAKAKEIAAGYAKIYNIPMSTGN
                     IDPIRIEAKKTMVFEWMRQTGKLPDVYIQAVSGGTGPLAIDKGIRELNQIMPELKNPR
                     MILVQTDGCDPMVQAWEKAEAESFPEGWEKKYPIIPNPKTEVPTLATGNPATYPIIAR
                     LVKKTGGTFIRMKEEKLVPLGKLISYEQKVIPGPASAVCVAGYFQALEKNLIKDGETV
                     IVNIGEGPNRAPLFLEQMIYTTCEVESVEQCKPHEINGFREELWKEVAPYPR"
     gene            complement(2537..3799)
                     /locus_tag="PLN75_08275"
     CDS             complement(2537..3799)
                     /locus_tag="PLN75_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRG04151.1"
                     /translation="MLDIFDRLKGMEGPLEQYRRKAEGYFMFPELEGEIGPRMKFHGR
                     EYITWSLNNYLGLANHPEVRKADADAAAQWGMAYPMGARMMSGQTKYHRELEQKLAAF
                     EKKESAYLLNYGYQGMVSIIDSLCTRNDVIVYDSESHACIMDGIFLHKAKGGKSFVFP
                     HNDMERCDKMLKFATKQAEENNGGILVITEGVFGMSGDLGYLPGIVELRKKYNFRLMI
                     DDAHGFGTMGETGAGASEHFGLMDEVDIYFATFAKSMAGIGAFVASDKPIVDSLKYNM
                     RSQIFAKSLPMPMVIGALKRFEILQNQPEHKENLWKIAKALQEGLVAEGFNIGNTQSP
                     VTPVYFSGSVPEGTNIVIDLRENHNVFCSIVVYPVVPKGVLMLRLIPTASHTLQDVND
                     TIEVFKKIKIKLEAGAYISDEVQSRALR"
BASE COUNT         1158 a         1035 c          987 g         1333 t
ORIGIN      
        1 ccccgaaggg atagcccccc caccccccga aggggggatg ttggtagtgg taggattatt
       61 tgaagtattt aatgcttgat tattcattta tttgagtatt tgggttactt taattggtca
      121 cttaaaaagt ccccgttcgg ggtcccgata gctatcgcgg atagggggct ttcacgttgc
      181 tttcactttc ctcaccattt ttcttgctaa tcccggtaaa aaccgtttga tatacaccat
      241 cagcaactct ttgccgccaa ccagcacttc cggtttttgc attttgatgg cttttactat
      301 ttttcgggct gctttttcgg ccgaaatgcc acccgcttgc ccgtcgtcca tttgggcgta
      361 tttggtgccg tcggcttcga gtgcattgaa ggaaatattg gttttcaccc gtcccggaca
      421 aaccattacc acacggatgt tttgttcgta atattcggct tgcacggttt caaaaaagcc
      481 gtaaagtgca aatttggacg ccgcgtaagc tgagcgaagg ggaaatccga attttcctgt
      541 aatactggtg gtaacagcta tagtaccgcc accacaagta atcatctcag ggagtattct
      601 tttggcaatt tttacgggtg cgtaaaaatt cacatccatg atttttttat ccaccgagat
      661 atcggtgtcg attgctttgg aacgctgact gataccggca ttaagaaaaa ggatatctat
      721 ttttccaaat attgaaagtg ctttatcggc aattgcatcc aggcggtcaa tttgtaaaag
      781 gtcgcagggc agaatctcac attcagcacc taattcgatg catttttgct gaactttctt
      841 caattgttct tcacgggttg ccgtcagaat cagcttcgca ccttccgacg cgaataaata
      901 cgaacacgct tcgccgatgc ccgacgaagc gcctgtgatc cagatggtag cccccccacc
      961 ccccgaaggg gggataagag tgcttttatc agaattatct gttacaatag agttagattt
     1021 gaccatataa tattttcatt acacaataat cttacattgt cgcagtagaa gtcccccatg
     1081 ggggtcatga tagctatcga ggataggggg ctacttcctt ccacaattct tctctgaatc
     1141 cgtttatttc gtggggtttg cattgttcca cgctttccac ttcgcaggtg gtataaatca
     1201 tttgttccaa aaagagggga gcacggttcg gcccctcgcc gatgttgaca atgaccgttt
     1261 cgccatcttt aatcagattt ttttccaaag cctgaaaata gcctgccaca cacactgcgg
     1321 aagccggacc cggaataact ttttgctcgt atgatatcaa ttttcccaag ggcacaagct
     1381 tttcctcttt catccggata aatgttccac ccgttttctt taccagcctg gcgataatcg
     1441 ggtaggttgc cggattgccg gtagccaggg taggtacttc tgttttggga ttaggaatga
     1501 tgggatattt tttttcccac ccttcgggaa aactttcggc ttcggctttt tcccacgcct
     1561 gcaccatcgg atcacagcca tcggtttgta ccaaaatcat tcgtggattt ttcagttcag
     1621 gcataatctg attgagttcg cgaatgcctt tgtcaatagc cagtggacct gttccaccac
     1681 tcacagcctg tatgtacacg tcgggcaatt tacctgtctg gcgcatccat tcgaacacca
     1741 tcgtcttttt ggcttcaata cgaatcgggt caatattgcc ggtggacatg gggatgttgt
     1801 aaattttggc atagccggct gctatttcct tggctttggc atagtcgccc aacacgcgga
     1861 aaacctgctg tccgtaagag ctgatggtcg cttccgaagt tttgattgca tcttcgggca
     1921 tgaatacgga gcagttaaca tttgccatcg ataagtattt ggcgtaagct gtggccgaat
     1981 ttccggtgga ggcaatgcag tattgtttca atcctatttc gttgaaaata cttgccgcaa
     2041 gtgaggctgc aatgtccttg aaagttccgg tacctccgtt caggtcattg cggtacacat
     2101 gcacttcgat attgagattg tatcgctctt ttgcaaaatt ttccagaaaa gcccaccttt
     2161 cgagtggcac agctccttca ttcctcgaaa tgatgtgacg tttacgaagc aacggcaaaa
     2221 aggtaaaata atgccagaaa ctattgggag tgcttttcca caaccgcaga agctttttgt
     2281 aattgcggcg ataccacact tcggaatgtt tacttccaca tttcggacat ttctgttcct
     2341 gcccaaacca cgtccagaaa tcgggtgtaa cgtaaccaca ttctgcacat ttcagttcgt
     2401 atttgtattg aatttttatt cgtaacataa ttttataaac gagttgacaa gtaaacaagt
     2461 acactttgaa agacaattta accaaacact caaagtgccc ttgcgggtac cgatagctat
     2521 cgtggacagg ggacttttat ctcagcgccc tggattgaac ttcatcagaa atatatgcgc
     2581 cggcttcaag tttgatcttg atttttttga acacttcgat agtgtcgttt acatcctgca
     2641 aggtatgcga ggcagtgggg ataaggcgaa gcatgagcac acctttgggc actacggggt
     2701 aaaccacgat agaacagaaa acattgtgat tttcgcgcag atcgattacg atgttggtac
     2761 cttcgggcac cgaaccgctg aagtaaacag gtgtgacggg cgattgtgtg ttgcctatat
     2821 taaatccttc ggctacaagg ccttcctgca atgctttggc tattttccag aggttttctt
     2881 tgtgctcagg ttgattttgc agaatttcaa aacgcttgag cgcacctata accatgggca
     2941 tgggtagtga tttggcaaaa atctgcgaac gcatgttgta tttcagcgag tcgacaatcg
     3001 gtttgtcgga agccacaaag gcaccgattc cggccatgga tttggcaaag gtggcaaaat
     3061 agatatccac ttcgtccatc agtccgaaat gctcgcttgc tccggctccg gtttcaccca
     3121 tagtaccaaa tccgtgagca tcgtctatca tcaggcggaa attgtatttc tttcgcagct
     3181 ccacgatacc cggcagatag cccagatcgc ccgacatacc gaagactcct tcggtaatca
     3241 ccagaattcc gccgttattt tcttcagcct gtttggtggc aaatttcagc attttatcgc
     3301 agcgttccat atcgttgtgc gggaatacaa agctctttcc gcctttggct ttgtgcagaa
     3361 aaatgccgtc catgatgcag gcgtgcgact ctgaatcgta cacgatcaca tcgttgcgtg
     3421 tgcagagtga atctatgatg gaaaccattc cctggtagcc gtagttgagt aaataagccg
     3481 attctttttt ctcgaaagcg gccagctttt gttccagctc ccgatggtat tttgtctgac
     3541 cggacatcat acgggcgccc atcggataag ccatgcccca ctgtgcggct gcatcggcat
     3601 cggctttacg cacttcggga tgattagcaa ggccgaggta attattaaga ctccaggtga
     3661 tatactcacg cccgtgaaac ttcattctcg gtcctatttc tccctccagt tcggggaaca
     3721 taaaataacc ctccgctttg cggcgatact gctccagcgg cccttccatg cctttcagac
     3781 gatcaaaaat atctaacata attttgctgt attttggtga aaattaattt taacagacaa
     3841 aaatagcttt tccgcgttac atttcaaaat ataatgaata aaaaagctcc gaacggatcc
     3901 cgtgcagccg gatggattat atttcttatt tcaacaaata tccctgtgcg ctgcacctga
     3961 aaaacaaata aaatacaaca gtttagaaat tattgaggag cgtagctgcg attcattatt
     4021 agaatacagc attaaagaat ttaatgaggt gggtatggta cattccgact tgacagaagc
     4081 atcgattctg ttatatttat caaactacga atgacacaaa ttacacgaaa aaattcaata
     4141 catagaattt tttgagcagc gttgcaccga ttctgatttc aaatagcaca ttgcggttcc
     4201 aaatagcaca ttgcggtttg aaaccgcatt gtgcatttaa gggaaaagag aatcaataag
     4261 gttttatttc cggttattcg gattactact aaggttcaaa tgtttgaatc agtgccacgc
     4321 accttgaatt gattgtattt tttatttcta caaatgaatc ggtgcgctgc accaaataca
     4381 aaaaacattc actatgatca gacgttgcat tcaccaaaag tcccccatgg gggatttagg
     4441 gggccgtttc cttatttctg cggtttgaaa ccgcattgtg catttaaggg aaaagagaat
     4501 caataaggtt tta
//
LOCUS       DAORKP010000164         4491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4771_length_4491_cov_6.751578, whole genome shotgun sequence.
ACCESSION   DAORKP010000164 DAORKP010000000
VERSION     DAORKP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_4491_cov_6.751578"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1310)
                     /locus_tag="PLN75_08280"
     CDS             complement(27..1310)
                     /locus_tag="PLN75_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRG04152.1"
                     /translation="MDLEECLQIGLEQNFDIRLSRNNRQITDNNATRGNAGMLPTVDL
                     SSSFGTSASGSNQIPLDGSDKIKIRGSHTETLNAGVNLNWMLFEGFRAQTSYKKLQEL
                     QKMGELNTQSVVENFIADLSAEYYNYVQQNIRMKNLRSAVRLSRERLRIVEARYQIGN
                     MSRLDLQQARVDFNTDSSRLMHQYELLNTSRIKLNELMGNQDVDRQFQTTDTAILVVT
                     ILDKKALQERIYTDNILMKLAEKDKVISELDLKTALSQNFPYLRLNAGYGLTHFNYNR
                     GNLDKQNTWGPNVGLTLGFRLFDGFNKAREQNNARIQIKNRELEQDRLKLSLNSQFTN
                     IWLSYRNNIELLSLEKISLENARLNYEIAMDRYKLGDLSGLQLREAQNNLQAAEERLV
                     SAEYSAKLYEISLMQISGRIGEYLSPSKPLQRGGF"
     gene            complement(1451..>4491)
                     /locus_tag="PLN75_08285"
     CDS             complement(1451..>4491)
                     /locus_tag="PLN75_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRG04153.1"
                     /translation="LSELSIKRPVLSTVFVLIILLLGGIGYTYLAVREYPSVDNPIIT
                     VSVSYPGANADVIEKQITEPLEQNINGIPGIRSLMSSSSQGNSRITVEFELSVDLETA
                     ANDVRDKVSRAQRYLPRDVDPPTVSKADADADPIMQITVQSPNRSLLELTEIAELTVK
                     ERLQTIQDVSAVEIWGENRYAMRLWLDPTKMAAYNITPLDVKNTLDRENIELPAGSIE
                     GDQIERSIRTMGLMITPEEFNNIILIENDKRIVRFRDIGYAELDSENRKNILRLNGIP
                     MVSTIIIPQPGANQINIADEARMRVQQMQKDLPEDVKVDVVFDNTRFIRASIAEVEST
                     VYVAFALVVIIIFLFLRNWRVTLIPALVIPVSLIGAFFVMYLAGFSINVLSMLAVVLA
                     VGLVVDDAIVVTENIYKKIELGMNPRKAAIEGSKEIFFAVVSTTITLVAVFFPIVFLE
                     GMTGRLFREFSIVISGSVLISSFVALTFTPMLSAKLLKKKEKHNWFQEKTEPFFEKMN
                     SGYENMLKSFIGRKWLVIPIIVVTIGLIFLFWNIIPAEMAPLEDRSQVTIRTSAPEGA
                     TYEFVRDYTGKISGIADSVAPERESNITISRGGFGMVRLTLPDIKERKRSQMEIADEL
                     TGAVRPETEARSFVQQQSTFGGRRGGMPIQYVLQAPNIQKLEEFIPLFMDEVNKSPYF
                     QMSDINLKFTKPETRIVIDRDKAALLGVSTREIGQTLQYALSGQRMGYFYMNGKQYQI
                     LAEINRQQRNTPLDLKSIYIKNSAGQMIQMDNVVQLVETVAPPQLYRYNRFNSATVSA
                     GLAPGYSLGQGIDEMDRIAKATLDETFRTALEGESRNFRESSSSLLFAFVLAILLIIL
                     ILAAQFESFKDPFIIMFTVPLAIGGALLFMWIAGVTMNIYSQIGLIMLIGLVAKNGIL
                     IVEFANQRQNAGMDKSEAILGASVLRLRPILMTSASTILGLLPLVFASGEGANGRIAM
                     GVAVVGGMLISTLLTLFIVPAMYMYISTKRNLPPTPLKGE"
BASE COUNT         1125 a         1051 c         1030 g         1285 t
ORIGIN      
        1 gctaatagct cccgcaattt tgaactttaa aaacctcccc tttggagagg tttggagggg
       61 cttaagtatt ccccaattct tccgctgatt tgcatcagtg atatttcata cagtttggca
      121 ctgtattcgg ccgaaaccag tcgctcctcg gctgcctgca gattgttttg cgcttcccga
      181 agttgtaagc cggataaatc acccaatttg tatctgtcca tcgcaatttc gtaattcaac
      241 cgggcatttt caaggctgat tttttcaaga gaaagcagtt cgatattatt ccggtaagac
      301 agccagatat tggtaaactg gctgttaagc gatagtttca accggtcctg ttctaattct
      361 ctgtttttaa tctgtatgcg tgcattgttc tgttcgcgtg ccttattgaa tccgtcgaaa
      421 agccgaaatc caagtgtcaa tcccacgtta gggccccacg tattttgttt gtccagattt
      481 ccccggttat aattgaaatg agttaaacca taaccggcat taagcctcag ataaggaaag
      541 ttctgactta aagccgtttt aaggtcgagt tcactgatca ctttatcttt ttcagccagt
      601 ttcatcagaa tattatcggt gtatattctt tcttgtagtg cctttttatc caggatagta
      661 acaacaagga tagccgtgtc ggtagtttga aattgcctgt cgacatcctg atttcccatt
      721 agttcgttca gttttatgcg cgatgtgttc agcaattcat actgatgcat cagtcgtgag
      781 ctgtcggtgt tgaagtcaac ccgtgcctgc tgcaagtcca gcctcgacat atttccaatc
      841 tgataacggg cttccactat tcgcagccgc tcacgcgaaa ggcgaactgc cgaacgcagg
      901 tttttcatcc gaatattttg ctgtacgtag ttgtagtatt ctgccgaaag gtcggctatg
      961 aaattctcaa cgactgattg cgtgttcagt tcgcccattt tttgcaattc ttgcaatttt
     1021 ttgtagctcg tttgggctct gaaaccttca aaaagcatcc agttcaagtt tacacctgca
     1081 ttcagcgttt cggtgtgtga accgcggatt tttattttat cgcttccgtc caagggtatt
     1141 tgattgcttc cgcttgccga agtaccgaaa ctgctgctga gatccactgt aggtagcatg
     1201 cctgcgttac cgcgtgtggc attgttgtcg gttatttgcc ggttgtttct cgaaagccgg
     1261 atatcgaaat tttgctccag tccgatttgc aggcattctt ccaaatccat tttttgctga
     1321 gaaaaagcaa gaagagggag gaaaagtaat aggcccccta accccctaaa gggggaaaac
     1381 ccgaaaaatg atactttagt tcttatactt tgttttttca ttttaaacgc aattcttaaa
     1441 tagtccacaa ctattccccc tttagggggg tagggggcag gtttctcttc gttgaaatat
     1501 acatatacat cgccgggacg ataaaaaggg taagcagtgt ggaaatcagc atgccgccca
     1561 ccacggctac acccatcgca atccggccgt tagcgccttc gcccgaagca aaaaccaacg
     1621 gcagtaatcc taaaatggtg gaagcgcttg tcatcagaat cggtcgtaag cgcagcaccg
     1681 aggcacccaa tatggcttcg gacttatcca taccggcgtt ttggcgctga ttggcaaatt
     1741 ctacaatcag aatcccgttt ttagctacca gcccaatcag cataatgagt cctatctgac
     1801 tgtagatgtt catggtaaca ccggctatcc acatgaaaag cagcgctccg cctatggcca
     1861 gaggtaccgt gaacatgatg ataaacggat ctttgaaact ttcgaattga gcagccagaa
     1921 taagtataat gagcaatatg gcaagtacaa aagcaaaaag gaggctcgaa gaactttccc
     1981 tgaagttacg cgattctcct tcgagtgctg ttcggaaagt ttcgtcgaga gtggctttgg
     2041 caatccggtc catctcatca atgccctgcc cgagcgagta accgggagcc agtccggccg
     2101 aaacagtggc tgagttgaat cggttgtagc gatacagttg cggtggtgcc accgtttcta
     2161 cgagctgcac cacattgtcc atctgaatca tttgaccggc cgaattttta atataaatgg
     2221 atttcagatc aagtggtgtg ttgcgttgct ggcggtttat ttcggccagt atctgatatt
     2281 gttttccgtt catgtagaaa tagcccattc gctgtccgct caatgcatat tgcaaagtct
     2341 gaccaatttc gcgggtgctc actccaagca atgcagcttt atcccggtca atcacgatgc
     2401 gtgtttcggg ttttgtaaat ttcagattga tatcagacat ctggaagtac ggacttttgt
     2461 ttacttcgtc catgaaaagg gggataaatt cttcgagttt ctgaatgtta ggtgcctgca
     2521 gcacgtattg aatcggcatc cctccacggc gtccgccgaa agtggattgc tgctgaacaa
     2581 acgagcgggc ttcggtttcg ggtcgtacag ctccggtcag ttcgtcggct atttccattt
     2641 ggcttcgctt acgctctttg atatcgggta gcgtgagtcg taccatgccg aatccacccc
     2701 ggctgatggt aatgttcgat tctctttccg gtgcaaccga atcggctatg ccggaaattt
     2761 ttccggtata atctcttaca aattcatacg tagccccttc gggtgccgaa gtccggatgg
     2821 taacttgcga gcggtcttcc agaggcgcca tttcagccgg aataatgttc caaaaaagga
     2881 aaatcaggcc aatggtaacc actataatgg gaataaccag ccattttctg ccaataaaac
     2941 ttttcagcat gttttcgtat ccggaattca ttttctcgaa aaatggttct gttttttcct
     3001 gaaaccagtt gtgtttttct ttctttttga gcaatttagc cgaaagcatc ggggtaaaag
     3061 tcagtgctac gaacgaagaa ataagtaccg aacctgaaat tacaatgctg aattcgcgaa
     3121 aaagccgtcc ggtcattcct tccagaaaaa cgatggggaa aaacaccgca acgagtgtaa
     3181 tggtagtgga aacgactgca aagaaaattt ctttcgaacc ttcgatggca gctttccggg
     3241 gattcatgcc gagttctatt ttcttgtaaa tattttcggt aaccacgatg gcatcgtcca
     3301 ccaccagccc cacagccaga actactgcca gcatcgaaag tacgttgatg gaaaatccgg
     3361 ccagatacat cacgaaaaaa gcgccgatga gcgaaaccgg aatgactaaa gcgggaatca
     3421 acgttacccg ccagtttcga aggaaaagaa atattattat cactacaagc gcaaatgcaa
     3481 catataccgt ggattctact tcggctatag atgccctgat aaatcgggta ttgtcaaaaa
     3541 ccacatccac cttcacatct tcgggcaggt ctttttgcat ctgttgcaca cgcatgcggg
     3601 cttcgtcggc aatgtttatc tgattagcgc cgggctgagg aatgataatc gtacttacca
     3661 tcgggatacc atttagccgg aggatgtttt tgcggttttc gctgtccagt tctgcataac
     3721 caatatcgcg gaaacgaaca atccgtttgt cgttttcaat cagaataatg ttattaaatt
     3781 cttcgggagt aatcatcaaa cccatcgtac ggatggagcg ttcaatctga tcgccttcga
     3841 tgctgcctgc cggaagttca atgttttcgc ggtcgagcgt gttttttaca tccagtggtg
     3901 tgatgttgta agctgccatc ttggtcggat caagccacag acgcatggcg taccggtttt
     3961 cgccccaaat ttccactgca cttacatcct gaatggtttg aaggcgctcc tttaccgtca
     4021 gttcggcgat ttcggtgagt tccagcagcg accggttcgg actctgaaca gtgatttgca
     4081 tgatcggatc ggcatcggca tcggcttttg aaacggttgg cggatccaca tcgcgcggaa
     4141 gatagcgctg tgcccgcgat accttgtcgc gaacatcgtt ggcagcagtt tccaggtcca
     4201 cgctcagctc aaattcaacg gtaattcttg agttaccctg actgctcgaa ctcatgagcg
     4261 accggatgcc cggaattccg ttgatattct gctccagtgg ttcggtaatt tgtttttcga
     4321 tcacatcggc attggctccc ggataggata cggataccgt gatgattgga ttgtcaacac
     4381 ttggatattc cctaacagcc aaataggtgt agccaatgcc tcccagaagc aagatgatca
     4441 acacaaaaac tgtggacaat actggtcgtt ttatgctaag ttcggataga t
//
LOCUS       DAORKP010000165         4484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4782_length_4484_cov_7.895010, whole genome shotgun sequence.
ACCESSION   DAORKP010000165 DAORKP010000000
VERSION     DAORKP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_4484_cov_7.895010"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1156
                     /locus_tag="PLN75_08290"
     CDS             113..1156
                     /locus_tag="PLN75_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRG04154.1"
                     /translation="MEPETENKKPKIRMPAEWEPQSFVQLTWPHALTDWAYMLDEVTA
                     CFVNIAREIVNRQNLLVVCADKNEVREKLKKLPQKKIMYAEIPTNDTWARDHGGITVF
                     KNGKPVVLDFTFNGWGMKFAASHDNQITRKLFTENVFPESVKYKSRKNFVLEGGSIES
                     DGKGTILTTAECLLSENRNNLTKEKAEKKLKKYLGAERILWLENGFLKGDDTDSHIDT
                     LARFCSVDTIAYVKCTDENDEHYEALSRMENELKTMTLSGSANKYNLIPLPMADAVYD
                     ENDDRLPATYANFLIINGAVLMPTYNSTKDQIALQQLQKAFPDRKIIGIDCTALIKQH
                     GSLHCVTMQYSLP"
     gene            1353..>1513
                     /locus_tag="PLN75_08295"
     CDS             1353..>1513
                     /locus_tag="PLN75_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRG04155.1"
                     /translation="MKIALIQQSNTANKNENLEKLEKNIREVASQGAELVVLQELHNG
                     LYFCQTEDVS"
     assembly_gap    1514..1613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1614..1919
                     /locus_tag="PLN75_08300"
     CDS             <1614..1919
                     /locus_tag="PLN75_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRG04156.1"
                     /translation="QRDAWITVQRSHAVANGIPVISCNRVGYEADPSGITKGIQFWGT
                     SFIAGPQGEILTKTSDKKDENLVFDLDLNRTETVRRWWPFFRDRRIDAYHDLIKRWK"
     gene            2048..>4484
                     /locus_tag="PLN75_08305"
     CDS             2048..>4484
                     /locus_tag="PLN75_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRG04157.1"
                     /translation="MRNFTKQLFIPLILTVAFSVQGAIPTGYYHFALGKKKAELKTTL
                     HDISSPLTVLDYGSGPGCTWQGFFSTDRNADNTVQDMYSNIVRNFNGYNSIPDMAIEH
                     SFPKSWWGAYENVAYKDLFHLYPADAYTNGIKNNLPLGEVAGTLLLDNGKSKIGKNGF
                     GTVYTDNSFEPADEYKGDFARSYLYISTIYEDLAPLWNSPMLTKTVYPVWQPWAIDLL
                     VKWHNQDPVSDKELARNDSIYAIQGNRNPFIDFPELTSYIWGIDSTKAFTYAEETRAF
                     LVAPRRKTKLDFGVILLNVTKSLNLTVQGMNITGPVSFSFLRNSPALSLSVNSISADQ
                     AHAGYDLQVNYQPVNSGTTIDTLLIQGGGLTEITRIPVRGLATSEFIVTEPTDVTPVG
                     GTLHWLEDPAATNYTVSLYQGDTRAGNLIISGYLEGTSYDKALEIYNGTGNDVDLSKY
                     TLKKQSNGYGDYETSYKLSGTLRNNKTLILAHASSTNETLRSKATLFTDSVLNFNGND
                     AIALFRNGVMVDVIGKLNGGADYSWGFDKILKRNADVTHPAANFDLNQWTEYPYTQLD
                     KIGTHVVNFASANNYIFKDKPVGITNHLTVDNLNPNENYTYSVTSYRSGVVAPSVNTQ
                     RLRTAGLEIPVGMAATDISGTSFTANWEETPYAAEYYLDVYQLSGEIKTETEGFNNVG
                     TNGKTLPDGWTGTASGNYTTAASSGINIPSIAFKNNLEWLQTKQFPNIIRKISFMYRY
                     PSAGTGSYFTVEGQNQSGWSLIETIPYNASTAKYYPSYTFSPEKGYTAIKFTYTKVTG
                     NLALDDVTIEHGKV"
BASE COUNT         1417 a          922 c          916 g         1129 t
ORIGIN      
        1 ttggtggacg aaggtgtgaa atggaagaga attgatattt agtcgaatag tcgaatagtt
       61 gaatagttga atagttgaat agtaaattgt aaataaaagg tgtcttacta acatggaacc
      121 tgaaactgaa aacaagaaac ccaaaattag gatgcctgcc gaatgggaac cacaatcgtt
      181 tgtacagctc acctggccgc atgccctcac cgactgggca tacatgctgg atgaagtaac
      241 agcatgtttt gtaaacatag cgcgtgaaat tgtaaatcgt cagaatctgc tggtggtttg
      301 tgctgataaa aacgaagttc gtgaaaaact aaaaaaatta ccgcagaaaa aaatcatgta
      361 cgcagagatt cccaccaacg atacctgggc gcgggatcac ggaggcatta cggtcttcaa
      421 aaacgggaaa cccgttgtgc ttgattttac attcaacggt tggggaatga aatttgctgc
      481 aagtcacgac aatcagatta cgcggaaatt gtttactgaa aacgtattcc ccgaaagtgt
      541 gaaatacaaa agcagaaaaa attttgttct cgaaggtgga agcattgaaa gcgatggcaa
      601 aggaaccatt ctgacaacag cagaatgtct gctttctgaa aaccggaata atctgaccaa
      661 agaaaaagca gagaaaaaac tgaaaaaata cctgggcgca gagcggattc tctggcttga
      721 aaacggattt ctgaaaggag acgataccga tagtcacatc gatacactgg cgcgattttg
      781 ctccgtcgat accatagctt acgtgaaatg taccgacgaa aacgacgaac attatgaggc
      841 gctgagcagg atggaaaatg aactgaaaac gatgacattg tccggaagcg ctaacaaata
      901 caacctgatt ccgcttccga tggccgatgc tgtgtacgat gaaaacgacg accgccttcc
      961 ggcaacttac gctaattttc tgattatcaa tggagcagta ctgatgccaa cctacaattc
     1021 tacaaaggac caaatcgctt tgcagcaact gcaaaaagca tttcccgatc ggaaaatcat
     1081 cggaattgac tgtacggcac tgattaaaca gcatggatcg ctccattgcg ttacgatgca
     1141 atacagcctc ccctaacccc tccaaaggag ggggatttgg ttctgcactc attgaattat
     1201 tgagtaaaaa acgtggatgt aatttctgtg actcaattat tggggaaaaa aatttgaaaa
     1261 tatttttctt aactcggtaa aagaatattg ttatacaaaa cttacaatta aaaactaatt
     1321 tccttccctt tggggaggtt aggaggggct atatgaaaat cgcacttatc caacaatcca
     1381 acacagccaa caagaatgaa aacctggaga agttagaaaa aaacatccgc gaagttgcat
     1441 cgcagggtgc agaacttgta gtacttcagg aacttcacaa cggattgtat ttctgtcaga
     1501 cggaagatgt gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagcgcg
     1621 atgcctggat cactgttcag cgatcacatg ctgttgccaa cgggattcct gtgatttcat
     1681 gcaacagggt agggtatgaa gctgatcctt ccggtattac aaaaggaatt cagttctggg
     1741 gtacaagttt cattgccgga ccgcaaggtg aaatcctgac caaaacttcc gataaaaagg
     1801 atgaaaattt agtttttgat ttagacttaa acagaactga aacagttcgc cgctggtggc
     1861 cttttttccg tgatcgaaga attgatgcct accacgacct tatcaaaagg tggaaatgaa
     1921 ataatataca accggtaaag tattaacaac tctttttgtc aaagaatgat aaaaagagtt
     1981 ttttcttttt gctgcctgat caaaataact tatatttgaa ctttctaaca tccgaagaat
     2041 taagccgatg cgcaatttta caaaacaact tttcatacca ttaatattaa ccgtcgcttt
     2101 ttcagttcag ggagccattc caacgggtta ctatcatttt gccctgggaa agaaaaaggc
     2161 cgaactgaaa acaacccttc acgatatctc atcacccctc actgtgctcg attatggaag
     2221 cggaccgggc tgtacatggc agggattctt ttctaccgac cgtaatgccg ataataccgt
     2281 gcaagatatg tattccaata ttgtccggaa tttcaatggt tacaattcca tccccgatat
     2341 ggccatcgag cactcattcc ctaaaagttg gtggggagct tatgaaaatg tggcttacaa
     2401 agaccttttt cacctctatc cggctgacgc atacaccaac gggataaaaa ataatctgcc
     2461 tttgggagaa gttgcaggta ctcttttact tgataatgga aaatcgaaaa taggaaagaa
     2521 tggtttcgga acagtatata ctgataatag ttttgaacct gcagatgaat acaagggcga
     2581 ttttgcacgt tcatacctgt atatcagcac aatctatgaa gatctggctc cactctggaa
     2641 ttcgcccatg ctcactaaaa cagtatatcc tgtttggcag ccctgggcta tcgatttact
     2701 tgtgaaatgg cacaatcaag acccggtaag cgataaagaa ttagcacgca atgacagcat
     2761 atatgccata cagggcaatc gcaatccatt tatcgatttt cccgaactga cttcatatat
     2821 atggggaatt gatagtacca aagcatttac ctacgccgaa gaaacaaggg cgtttctggt
     2881 ggcaccacga agaaaaacca aactggattt cggagttatt ctgctgaatg taacaaaatc
     2941 actgaatctc actgttcagg gaatgaatat cacaggaccg gtttcctttt cattcctcag
     3001 aaacagtccg gcattatccc tttcggtaaa ttccatatca gctgatcagg cacatgccgg
     3061 atatgattta caggtaaatt accagcctgt caattcgggc actaccattg atacgttgct
     3121 cattcagggt ggaggtctta ctgagattac ccgaattcct gtccggggac tggctacttc
     3181 cgaatttatt gtcacagaac ctaccgatgt tacacccgtt ggcggcactc tccattggct
     3241 ggaagatccc gctgccacca attatacagt ttcgctgtat cagggagata cccgggccgg
     3301 aaatctgatt atttcgggat acctggaagg tacatcgtac gacaaagcac tcgaaattta
     3361 taacggaacc ggaaatgacg tagatttatc gaaatatact ctgaaaaaac agtccaatgg
     3421 ttatggtgac tatgaaacat cctacaaact ttccggtact ctccggaata ataaaacact
     3481 gatactcgca catgcctctt cgaccaacga aacacttcgt tcaaaagcta cattatttac
     3541 tgattctgtg ttaaatttca atggtaacga tgccattgcc ttattcagaa acggagtgat
     3601 ggtagatgta ataggaaaat taaatggcgg agctgattac agttggggct tcgataaaat
     3661 cctcaaaaga aatgccgacg taactcatcc tgctgcaaat tttgatttaa accaatggac
     3721 tgaatatcct tacacccagc tggataaaat aggaactcac gtcgtgaact ttgcatcggc
     3781 caataattat attttcaaag ataaaccggt gggaattacc aatcatctga cagttgacaa
     3841 cttaaatccc aacgaaaatt atacctacag cgtcacctct tacaggagcg gagtggtagc
     3901 accctcggtc aatacccagc gactgcgaac cgccggtttg gaaattccag tagggatggc
     3961 agctacagat atcagcggca catccttcac tgccaactgg gaagaaacgc cttacgctgc
     4021 ggaatattac ctcgatgtat atcaactgtc cggagaaatt aaaaccgaaa ccgaagggtt
     4081 taataatgta ggtacaaatg gaaagacact tccggacggt tggaccggaa cagcgagtgg
     4141 aaactatact acagctgcaa gttcgggtat aaatattcct tccattgcat ttaaaaataa
     4201 ccttgaatgg cttcaaacca aacaattccc gaacataatc cgtaaaatta gttttatgta
     4261 ccgttatcct tcagccggaa caggttctta ttttactgtt gaagggcaaa atcaatcggg
     4321 atggtcattg attgaaacca taccatacaa tgcaagcaca gcaaaatatt atccatctta
     4381 tactttttca cctgagaaag gttacactgc aataaaattc acttatacaa aagtcacagg
     4441 aaacttagca ctggacgatg tgaccattga acacgggaaa gtgg
//
LOCUS       DAORKP010000166         4473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4805_length_4473_cov_9.775238, whole genome shotgun sequence.
ACCESSION   DAORKP010000166 DAORKP010000000
VERSION     DAORKP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_4473_cov_9.775238"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLN75_08310"
     CDS             complement(<1..314)
                     /locus_tag="PLN75_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRG04158.1"
                     /translation="MLEIKNLHATVNGKEILKGVDLTVKKGEVHAIMGPNGSGKSTLA
                     SVITGNPAFEVTKGKVIYEGVDLLELSPEERAGAGVFLSFQYPVEIPGVSMVNFMRAA
                     IN"
     gene            complement(477..1913)
                     /gene="sufB"
                     /locus_tag="PLN75_08315"
     CDS             complement(477..1913)
                     /gene="sufB"
                     /locus_tag="PLN75_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRG04159.1"
                     /translation="MSDEILYEVTQSEYKYGFTTDIETDIIPPGLNEDVVRLISAKKN
                     EPDWLLEFRLKAYRHWTKMEMPDWAYLKIPPIDYQAISYFAAPRKNAPKSLDEVDPEL
                     LKTFDKLGIPLEEQKILSGMAVDAVMDSVSVKTTFRENLAELGIIFCSFSEAVQEHPD
                     LVQKYMATVVPYADNYFAALNSAVFSDGSFAYIPKGVRCPMELSTYFRINAANTGQFE
                     RTLIVAEEGSYVSYLEGCTAPMRDENQLHAAIVEIVAMKDAEVKYSTVQNWYPGDKEG
                     KGGVYNFVTKRGICKGENSRISWTQVETGSAITWKYPSCILMGDNSSAEFYSVAVTNN
                     HQQADTGTKMIHIGKNTSSHILSKGISAGVSNNSYRGLVKINPKAENARNFSQCDSLL
                     LGDKCGAHTFPYMEVNNDTAIVEHEATTSKISEDQIFYCNQRGIDTETAVGLIVNGYA
                     KEVLNKLPMEFAVEAQKLLQITLEGSVG"
     gene            complement(2250..3251)
                     /locus_tag="PLN75_08320"
     CDS             complement(2250..3251)
                     /locus_tag="PLN75_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRG04160.1"
                     /translation="MSFLTNDKLVIVGAAGMIGSNMAQTAAMMRLTPNICLYDPFPTG
                     LEGVSEEIRHCGFEGLNLTYTTDIKEAFTDAKYIVSSGGAPRKDGMTREDLLKGNCEI
                     AEQLGKDIKAYCPDVNHVVIIFNPADITGLVTLMHSGLKPNQVTTLAGLDSTRLQSEL
                     AKHFGVKQSEVTNTRTYGGHGETMAVFASTAKVNGTPLLELVGTEKLTKEEWADLKTR
                     VTKGGANIIKLRGRSSFQSPSYVSIEMIRAVMGGEAFRWPSGCYVSNGEFDHIMMALE
                     TTLDKNGVSYGEIKGTEEEMTDLRTSYSHLRKLVDEVIGFGILPAYEDWNKLNPNIK"
     gene            3608..4450
                     /gene="nfo"
                     /locus_tag="PLN75_08325"
     CDS             3608..4450
                     /gene="nfo"
                     /locus_tag="PLN75_08325"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRG04161.1"
                     /translation="MKYIGAHVSAAGGVENAPLNAQKIGANAFALFTKNQKQWVAPPL
                     TEKSIELFKLRCEEFGFLSEHILPHDSYLINLGSPDPESLDKSRSSFVVEMQRCEQLG
                     LKKLNFHPGAHLNKISIDDCISIIATSINIALEKTNGVIAVIENTAGQGSAIGHNFEH
                     LAQIIDQVEDKSRVGVCLDTAHTLAAGYEIRTKDGFEETFRKFDDIVGFNYLQGMHIN
                     DSKKELATRVDRHDSLGKGFMGMDTFSFIVNDSRFDNLPLILETPDETLWAEEIALLF
                     SLAK"
BASE COUNT         1216 a          985 c          937 g         1335 t
ORIGIN      
        1 tcgttgatgg cagcccgcat aaaattgacc atgctcacac ccggtatttc caccggatac
       61 tgaaaactca ggaagacacc tgctcctgcg cgctcttcgg gcgaaagttc gagcaaatct
      121 actccttcgt aaataacttt tcctttagtc acttcaaatg ccggattacc ggtaatcacc
      181 gatgccagtg ttgattttcc ggatccattc ggacccataa tagcgtgaac ttcgcctttt
      241 tttacggtca aatccactcc ttttaatatc tctttaccgt tgacggtagc gtgtaagttt
      301 ttgatttcta acataaagcc cctccaaacc tccccgaagg ggagtctttt aaaaccctca
      361 aatatgagag tttttgattg ttattttgtg taatgtatct atttaataaa tcgagaatat
      421 gtaagcgcaa aggcactatc cctaagcctc ccttttgggg aggttggtgg ggctttttac
      481 cccacacttc cttccaatgt aatctgcaac aatttctgtg cttctacggc aaattccatc
      541 ggaagtttat tcagtacttc tttggcgtag ccgtttacta tcaatccaac agcagtttcg
      601 gtatcaattc cgcgctggtt acaataaaaa atctggtctt cgctgatttt tgacgtggtg
      661 gcttcgtgct ctacgatggc tgtatcattg tttacttcca tatacgggaa tgtgtgtgca
      721 ccgcatttgt cgccaagcaa caagctatcg cattgtgaga agttacgtgc attttcagct
      781 tttggattga ttttaaccaa accgcggtag ctgttgttgc tcacaccagc cgaaattcct
      841 ttggaaagta tgtggctcga cgtgttttta ccaatgtgga tcattttggt tcctgtatcg
      901 gcttgctggt ggttgttggt aacagccacg gagtaaaatt cggcagatga attatcgccc
      961 atcagaatgc acgacggata tttccaggta atagccgaac cggtttcaac ctgcgtccat
     1021 gagatgcggg agttttcacc cttacaaata ccgcgtttgg ttacaaaatt gtaaaccccg
     1081 cctttgcctt ctttgtcgcc cggataccag ttctgtaccg ttgaatattt aacttcggca
     1141 tctttcatag ctacaatttc cactatggca gcatggagct gattttcatc gcgcataggt
     1201 gctgtacagc cttccaggta agaaacgtag cttccttctt cggctacaat caatgtacgt
     1261 tcgaactgac cggtatttgc agcattaatt cggaaataag tggaaagttc catcggacag
     1321 cgcacacctt tcggaatgta agcgaatgaa ccatcgctga aaacagctga attgagcgct
     1381 gcaaagtaat tatcggcata aggaactacc gtagccatgt atttttgtac caaatcagga
     1441 tgctcctgaa ccgcttcgct gaacgaacag aaaataatgc ccagctcagc cagattctcg
     1501 cgaaaagtag ttttcactga aacgctgtcc atcacagcat ctacggccat tcccgaaaga
     1561 attttctgct cttcaagcgg aattccgagt ttatcaaaag ttttgagcag ttccggatcc
     1621 acttcgtcca ggctttttgg agcgtttttc cgcggagcgg caaagtatga aatcgcctga
     1681 taatcaatcg ggggaatttt caaatacgcc caatccggca tttccatctt cgtccagtga
     1741 cggtacgctt tcagtcggaa ttcgagcagc caatccggtt cgttcttctt cgcagaaatc
     1801 aatcgcacca cgtcttcatt cagcccagga ggaataatat cagtctcaat atcggttgta
     1861 aatccgtatt tgtactcgct ctgggttact tcatataata tttcgtcaga cattttttaa
     1921 aattagaagt tagagattag aaatctgaaa taagaaaatt gttccggtat agctttgctc
     1981 atcgaaatgt ttgcaatatg ttgtcacatt gccatcaaag atagaaaaaa ttatttcaga
     2041 tttatttaca caacaatagc tttcatttct ccaatgaaat agtagtgtaa taacaacagg
     2101 aagtggaaat tgttttaaat tagtcgtggc taatgtgaat gaaaaatgcg tatcaaaatt
     2161 actcctgata cgcatttttg aaaaaacatt cgacctcttg ctctcacaat tcactttcgg
     2221 ttgcagcgaa aggaataggg ataagggctt tatttgatat tcgggttcag tttgttccag
     2281 tcttcgtaag caggtaagat accgaaaccg attacttcgt ccaccagttt gcgaaggtga
     2341 gagtagcttg tgcgaaggtc tgtcatttct tcttctgtac ctttgatttc gccataagaa
     2401 acgccgtttt tgtcaagcgt agtttccaat gccatcatga tgtggtcaaa ttcgccgttg
     2461 cttacatagc aaccggaagg ccaacgaaat gcttcgccac ccatcaccgc acgaatcatt
     2521 tcgatagaaa catatgaggg actttggaaa gatgagcgtc cgcgaagttt gatgatatta
     2581 gcaccacctt ttgttacgcg ggttttaaga tctgcccact cttcttttgt aagtttttct
     2641 gttccaacga gttcaagaag tggcgtgccg tttacttttg cagttgaagc aaatactgcc
     2701 atagtctcac cgtgaccgcc ataggtacgt gtattggtca cttcgctctg ttttacgccg
     2761 aagtgttttg ccagttcgct ttgcagacgc gtgctgtcca aacctgccaa agtagtcacc
     2821 tgattaggtt tcaggccgct gtgcatcaat gtaaccaaac ctgtgatatc agccgggtta
     2881 aaaatgataa ccacatgatt tacatcaggg cagtatgcct tgatgtcttt acccaattgc
     2941 tctgcgatct cacagtttcc tttcagcagg tcttcgcgtg tcattccgtc tttgcgcggc
     3001 gcgccaccgg aagaaacgat gtattttgca tcggtaaatg cttctttgat atcggtagta
     3061 taagtcaggt tcaacccttc gaaaccacag tggcggattt cttcggatac accttccaat
     3121 cctgttggaa atgggtcgta aaggcaaatg tttggagtta acctcatcat agctgctgtt
     3181 tgtgccatgt tggatccgat cataccggct gcgcctacga ttactaattt gtcgtttgtt
     3241 aaaaagctca taatcttttt gttataatgg ttaataaatt tcaattttga tagtgcaaat
     3301 ttactcatta ttattgacat ggaaaagata atgcaaaact atttaatgaa atttttctct
     3361 aattaaaaaa aggcactttt ctgacctgaa aacgtcattt aattgacatt tcaagaaaca
     3421 atgtttatct ttgtttgtta gaaaatagat tgtaagaccg cttcgctgat agaacacaga
     3481 ctgcattcaa cactaaaaag tcttgattct aaaagcccag gctcccggtt ctttttccct
     3541 ggttcttgaa aatctattgt ctaacagtct attgtctaac agtctattgt ctaaaaatct
     3601 tgaggaaatg aaatacatcg gcgctcacgt aagtgctgca ggtggcgtgg aaaacgcacc
     3661 tcttaatgca caaaaaatag gtgcaaatgc ttttgcactt ttcaccaaaa accagaaaca
     3721 atgggtggct ccaccactga cagagaaaag catcgagctt ttcaagcttc gctgtgagga
     3781 attcggcttc ctgtcggaac atatccttcc gcacgacagc tatttaatca atctgggaag
     3841 tcctgaccct gaaagcctgg ataaatcccg gagttcgttt gttgtggaaa tgcagcgctg
     3901 tgaacagctt ggactgaaaa agctgaattt ccaccctgga gcacatttga acaaaatcag
     3961 catcgacgac tgcatcagta tcattgctac ttccatcaat attgctttgg agaagaccaa
     4021 tggcgtgatt gcagttatag aaaatacggc gggtcaaggt tcggccatcg ggcataattt
     4081 tgagcatttg gcacagataa tcgatcaggt agaggacaaa tcgcgcgttg gcgtttgctt
     4141 agatacggct catacccttg ctgcaggtta tgaaatccgc acaaaggatg gatttgaaga
     4201 gacttttcgg aaattcgatg atatcgtagg tttcaactac ctgcagggaa tgcatatcaa
     4261 cgacagcaag aaagaacttg ccacgcgcgt ggaccgccac gacagcctag gaaaaggatt
     4321 tatgggtatg gatacatttt cttttattgt taacgacagc cggtttgaca acctcccgct
     4381 gatactcgaa acgccggacg aaacgctgtg ggcagaggaa atagcgctgt tgttttcgct
     4441 ggcaaaataa acattgactt aaataatact aaa
//
LOCUS       DAORKP010000167         4428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4887_length_4428_cov_9.419392, whole genome shotgun sequence.
ACCESSION   DAORKP010000167 DAORKP010000000
VERSION     DAORKP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_4428_cov_9.419392"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLN75_08330"
     CDS             complement(<1..410)
                     /locus_tag="PLN75_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRG04162.1"
                     /translation="MKKVLTLMLIAVFMLSACSSSKKAALQSGKAIQPIQTVIPARPA
                     GQKHVLGLTTPKMDVVRVGFIGLGMRGPGAVDRFTHIPGVEIKALCDLYPERVENSQR
                     ILEKNGKPRATAYSGSEDAWKAMCERDDIDLIYIV"
     gene            662..2080
                     /locus_tag="PLN75_08335"
     CDS             662..2080
                     /locus_tag="PLN75_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRG04163.1"
                     /translation="MKLRSYLFMSLVAFTSLLAAQTEDNKLSIGLSGGLNEYNGDLGN
                     GLFKFKPINPLGALSLSTYVSPSFNLGIQGSFGKFGYKGSYLNPLMDATQDIEFGGNK
                     YDGFMFLEYKLNNGYLLSKESKFAPFLTAGIGLAGYLENNNDGMIQPFPVDLITPVGA
                     GLKFQFTDNLALQYKYLYSFTNHDEHDLTRMVDKNDGYGQHLLGVVISLGAKAKDTDG
                     DGVVDKKDMCPDTPAGVQVDASGCPLDRDGDGIADYQDECPDVPGLKALNGCPDSDGD
                     GVTDSKDLCPDTPAGVKVDAKGCPLDRDGDGVADYLDKCPDVVGVPLFNGCPDTDGDG
                     VPDYKDKCPTVAGPVENNGCPEEAHVLVQSLEGIQFEFDSDVIKPESYPTLDAAVETL
                     KSKTEFNLDVSGHTDNIGDDAYNQNLSERRANAVVKYLTDKGISAARLKAAGFGETQP
                     VAENETPEGRAKNRRVDFKVNY"
     gene            2215..4098
                     /locus_tag="PLN75_08340"
     CDS             2215..4098
                     /locus_tag="PLN75_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG04164.1"
                     /translation="MNKTEKPVKTRSPWAWIPTLYFAEGIPYVVAMTVAVIMYKRLGI
                     SNTDIALYTSWLYLPWVIKPFWSPFVDLIKTKRWWITTMQLLIGAGLAGVAFLIPAPF
                     FFQATLAVLWLIAFSSATHDIAADGFYMLALDSGQQSFFVGIRSTFYRLAMIAGQGVL
                     IILAGSLEVATGTEPLKIQVQSSNTAQSTTIIPDSLPTDEKRTSEVFTAFPASLTLST
                     TNISPEELAKIKDFAKVENEKNGFNTSETPKKVTEVQEKSSWWTAHISEPLGNYIKTR
                     FGEKRDTDIPLIVGQKTGNAGLIAVRLNRKPEPGKEMVLNTRFSSGDKSISLVSGERL
                     VFNENNWDKPAWLVVQLDKKLTTEVNGTFTAASGNLPLAWSITFYILAGFFLLIFIYH
                     RFALPHPASDAPKNHRASEIMREFGLTFKSFFSKEGIGLAITFMLLYRLGEAMLVKMA
                     SPFLLDTREVGGLGLTTGQVGLVYGTVGVISLTLGGIVGGIAASRKGLKYWIWPMALC
                     ITLPHLAYLYLSWFMPQNMFLVNMAVAIEQFGYGFGFTAYMLYLIYFSNGEHKTAHYA
                     ICTGFMALGMMLPGMIAGWIQELIGYNHFFIFVMICAIPTLAVIPFLKIDRSFGKKEI
                     AAK"
     gene            complement(4135..>4428)
                     /locus_tag="PLN75_08345"
     CDS             complement(4135..>4428)
                     /locus_tag="PLN75_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRG04165.1"
                     /translation="GNEIINRLVNHGFIEQMPDKDDRRSIRIRITDFGRSEVIKILPE
                     MQLVSRIVTGNLNQNEIRTLSYLLRKLDDYHNDIFLNKKEFGLKEVLASQNSG"
BASE COUNT         1265 a          946 c          994 g         1223 t
ORIGIN      
        1 acaatgtata tcaggtcaat atcatcgcgc tcgcacatgg ctttccaggc atcttcgctc
       61 ccggaataag ccgtagcacg cggtttgccg tttttttcaa gaatacgttg tgagttttcc
      121 actctctcgg gataaagatc acacagcgcc ttgatttcta ctccgggaat atgggtaaaa
      181 cgatctacag ctccgggacc acgcataccg agaccgatga aacctacgcg aacgacatcc
      241 attttgggag tagtaagtcc gagaacatgt ttttgtcctg caggacgagc gggaataact
      301 gtctgaatag gttggattgc cttgcccgat tggagcgcag cttttttgga agaactacat
      361 gccgataaca taaaaacagc tatcagcatc agagttaaaa cttttttcat agattattta
      421 atttttgtgg tttttttcaa cgtgcaaaag taagaatttt attgcaaaac tcctaaatat
      481 tttttcaaac aagtttgatt tgcaaaggat tgcgatttgg aattaagata taaagcatgt
      541 tttgagcctg tttttttgtt tttttaatta tttttgaaca ttatacttaa aacaagcatt
      601 ataacatagt aattaagaat tttcaattca ttattcaatt aattataaac aataaaagat
      661 tatgaaacta agaagttatt tattcatgtc gttggttgct tttacatcat tacttgcagc
      721 acagacagaa gacaacaaat tatcaatcgg tttgtcgggt ggtctgaatg agtataacgg
      781 tgatcttgga aacggtttgt tcaaatttaa gcccatcaat ccactgggtg cactttctct
      841 gtccacttac gtaagtcctt cgttcaacct gggaatacag ggcagttttg ggaaattcgg
      901 ttataaggga agctatttaa atccgttaat ggatgccact caggacattg agtttggcgg
      961 aaataaatat gacggattca tgtttctgga gtataaactg aacaacgggt atctgctgag
     1021 caaagaatcg aaatttgcac cattccttac ggcgggtatt gggttggccg gatacctgga
     1081 aaacaacaac gacggtatga ttcaaccttt ccccgttgat ttgattactc cggtaggtgc
     1141 aggtttgaaa ttccagttca ctgataatct ggctcttcag tataaatacc tgtatagttt
     1201 caccaatcac gacgaacatg acctgacccg catggttgac aaaaacgatg gctacggtca
     1261 acatcttctg ggcgtagtta tttcactggg tgctaaagct aaagataccg acggagacgg
     1321 agttgtagat aaaaaagaca tgtgtccgga tactccagcc ggtgttcagg tggatgcatc
     1381 aggatgtccg ctcgacagag atggcgacgg tatagccgac tatcaggatg aatgtccgga
     1441 tgtgccgggt ttgaaagcgc tcaacggatg tcccgacagc gatggcgatg gtgtaacaga
     1501 ctccaaagac ttatgccccg atactcctgc cggagtgaaa gtagatgcaa aaggatgccc
     1561 gctggaccgc gacggtgacg gcgtagctga ctacctggac aaatgtccgg atgttgtagg
     1621 cgtaccattg ttcaatggtt gtcctgatac agacggcgac ggtgttccgg attataagga
     1681 caaatgtccg acagtggccg gaccggttga aaataatggc tgccccgaag aagcacatgt
     1741 acttgtacag tcgctggaag gcattcagtt tgagtttgac agcgatgtaa tcaagccgga
     1801 atcttatccg acgctggatg ctgccgtaga aacgctgaag tcaaaaaccg aattcaatct
     1861 tgatgtgagc ggtcataccg ataacatcgg cgatgatgcc tacaaccaga atttatctga
     1921 acgtcgtgcc aatgcagtgg taaaatacct caccgataaa ggtatcagtg cagccaggct
     1981 gaaagctgca ggttttggcg aaacccaacc ggtagctgaa aatgaaactc cggaaggcag
     2041 ggctaaaaac cgccgcgtag atttcaaagt gaactattaa aatacaattt actgacaaaa
     2101 tgctccatcg atgattcggt ggagcatttt ttatttaaaa aatcttgaat ttgactcaaa
     2161 tgcaacaatc atttcttaca tttgcatgaa tcatttatcc ggaaaacacc cgctatgaat
     2221 aaaacagaaa aacctgtgaa aacacgttcg ccctgggcgt ggatacccac tctttatttt
     2281 gccgaaggaa taccctatgt cgtagccatg actgtagccg taatcatgta caagcggctc
     2341 ggcatatcaa acaccgacat tgccctttat accagctggc tgtatttacc ctgggttatc
     2401 aagcctttct ggagtccttt tgtagatctg ataaaaacaa aacgatggtg gattaccacc
     2461 atgcagctgc tcattggtgc agggctcgca ggtgttgctt ttttaattcc ggctccgttt
     2521 ttctttcagg ccaccctggc agttttgtgg ctgatcgctt tcagttctgc tactcacgat
     2581 attgccgccg acggatttta catgctggca ctcgatagtg gtcagcaatc attcttcgtg
     2641 ggaatacgca gcacatttta ccggctcgcc atgatagccg gacagggagt actcataatt
     2701 ctggccggaa gcctggaagt ggctaccgga acagaaccgc tcaaaatcca ggtacaatca
     2761 tccaatactg ctcaaagcac gacgattatt ccggattcat tgccgacgga tgaaaaacgt
     2821 acatccgaag tttttacggc ttttccagcg agcttaacat taagcactac gaatatttca
     2881 ccggaagaac tcgcaaaaat taaggatttt gcaaaggttg agaatgaaaa gaacggattc
     2941 aacacctctg aaacgccaaa aaaagtcaca gaagttcagg aaaaatcctc ctggtggacc
     3001 gctcatatat cagagccgct tggtaactat attaaaacca gatttggtga aaaaagagac
     3061 accgacatac ctttaattgt agggcaaaaa acgggtaatg ccggattaat tgctgtaaga
     3121 ctgaacagga aacccgaacc cggaaaagaa atggtactga atacgcgttt ctcatctggc
     3181 gataaaagca tatcgcttgt aagtggagaa aggctggttt tcaacgagaa taactgggat
     3241 aaacctgcct ggctggtagt gcagcttgac aagaaactga caaccgaagt gaatggaaca
     3301 ttcaccgctg catcgggaaa tctgccgctg gcctggagta taacatttta cattctggcc
     3361 ggttttttcc tgttgatatt tatctatcac cggtttgccc tgccacatcc agcgtccgac
     3421 gctcctaaaa atcaccgtgc aagcgaaatt atgagagagt tcggactgac tttcaaatca
     3481 ttcttctcaa aagagggaat aggacttgcc attactttta tgctacttta tcgtcttggt
     3541 gaagccatgc tggtgaaaat ggcatctccc ttcctgctcg acacacgcga agtgggaggt
     3601 ctcggactta caacgggaca ggttggactc gtttatggaa ccgtaggagt aatttcacta
     3661 accttaggcg ggatagtagg tggcattgcc gcatcacgta aaggactcaa atactggatc
     3721 tggcccatgg cactctgtat cacactacct catttggcat acctttacct gtcgtggttc
     3781 atgccgcaaa acatgtttct ggtaaatatg gcagtggcaa tcgaacaatt cgggtatgga
     3841 ttcgggttta cggcatacat gctgtatctt atctactttt caaacggcga gcacaaaacg
     3901 gctcattacg ccatctgcac cggctttatg gcactcggga tgatgctccc ggggatgatt
     3961 gccggatgga tacaggagtt gatcggatat aaccatttct tcatctttgt gatgatatgt
     4021 gccataccta cgctggcagt aattccgttc ctgaagattg acaggagttt cggaaaaaaa
     4081 gaaattgcag caaagtaaaa cgaatttata aaagaaacag ataatcctgt ttttttaacc
     4141 ggaattctga gatgctaaaa cttccttcaa cccgaattcc tttttattca ggaaaatatc
     4201 gttgtgataa tcgtcgagct tccgaagcag gtatgacagc gttctgattt cgttctgatt
     4261 cagatttccg gtcacaattc tggataccag ttgcatttcg ggtaaaattt taatcacttc
     4321 acttcttccg aaatcagtga ttctgattct tatgcttctt cggtcgtcct tatcgggcat
     4381 ctgctcgata aacccatgat tcacaagtct gttgataatt tcattgcc
//
LOCUS       DAORKP010000168         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_4922_length_4414_cov_9.407892, whole genome shotgun sequence.
ACCESSION   DAORKP010000168 DAORKP010000000
VERSION     DAORKP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_4414_cov_9.407892"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2150
                     /locus_tag="PLN75_08350"
     CDS             45..2150
                     /locus_tag="PLN75_08350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRG04166.1"
                     /translation="MKKLFLIMMIGTIVTACTGKSEKSDNPFFSDYKNEYKAPPFESI
                     KNAHYLPAFEEGIKQHQAEIDSIASNPEAPTFANTIEAMEYSGELLSKVSAVFSNLQS
                     AETNDEMDSIAKIVTPKLTEHSDNIYLNDKLFQRVRAIFDTKATLGLTPEQDRVLEKI
                     YKRFIRSGAALNEEQKAQLREINKELSTLELTFGQNILAETNAFKKFVTNKEELKGLP
                     EGIIEAAAEEAKKEGKEGQWLFTTSKTSFIPVLQYGENRELRKELLMAYVDRCNHDNQ
                     NDNKPVINKIMKLRVRKAQLLGFESPAAFILDNTMAKTPDAVYSFLNKVWTPAVAKAK
                     AEASELQKLMDAEGKGEKLEPWDWWFYTEKLRKAKFDLDEEQLRPYFKLENVRKGVFD
                     LTTKLFGLKYEKLEGMPVYHKDVEVFKVTDADGKLIGILYTDYFPRAGKTVGAWMSNF
                     QEQYIKDGVNQRPIIVNVGNFTKPTADKPSLLTMDEVETLFHEFGHALHGLLANSTYP
                     SLSGTNVARDFVELPSQIMENWCWEPEVMKTYALHYKTGEVMPQELMDKIRKAGTFNQ
                     GFVNTELLSASILDMDYHVKKDTSDIDVNAFEKASMDKMGMIPQIIVRYKSPIFKHIF
                     EGGYSAGYYSYTWAAVLDADAYQAFKETGDIYNKEVATAFRKNILEKGDSEEPMKLYR
                     TFRGADPNPDALLIKRGLK"
     gene            2297..3280
                     /locus_tag="PLN75_08355"
     CDS             2297..3280
                     /locus_tag="PLN75_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04167.1"
                     /translation="MKSDLQKLIVPSLPLTALIYVVSFVIWMLFYIIYKYPYPMPGKI
                     GSIAESFLSSQSLAAYLLSYAIVVVNSMVIAQMNNRFSFIRTRTFLPTFVYLLVSVCW
                     LPLHGNYVANLAAFFVLLAIFMSLVMYKMPNGVEQAFLSFFLLALSTFLVPDYAYLIV
                     LFWMGYFFLKCFSPRVFFASVFGFVTPWILYLSVIFFIFNEQGFVSDIPAFFYRYSVF
                     HYENIPAIIYVLLMILIVAISLVQMIAKSRQDSIQARNELNFLKLLFFGVAMLFVLRF
                     SNYISYLPLIAMFFSVFTAYTFTLIRNLFNFIVFIALVALSFLFAFYIIII"
     gene            3288..3728
                     /locus_tag="PLN75_08360"
     CDS             3288..3728
                     /locus_tag="PLN75_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRG04168.1"
                     /translation="MFDFAEWGYLGLFIASFLAATVVPFSSEVVFSAMVFGGLHPWTC
                     VLVATAGNWLGGMTSFYIGRLGKIEWVEKFLHIKKEKVERFVEKFSRYGDWFVFFSFV
                     PFVGDVIAVAAGFFRGRWWVVAIAMLLGKFIRYVIWMYANGMFM"
     gene            3831..4181
                     /locus_tag="PLN75_08365"
     CDS             3831..4181
                     /locus_tag="PLN75_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRG04169.1"
                     /translation="MKKIFFFAIFSLAIISLNAANKVKAEADAKANAEVSVQKNAING
                     RVIDAVTNETLAGAEISVNGQKIYSDLDGNFDIKDAKGEKIKITISMISYADQVVEIE
                     PGTHNLLNVKLKQL"
BASE COUNT         1264 a          905 c          948 g         1297 t
ORIGIN      
        1 gtaaaatata ttcttaaata ataatactgt aaaaaataaa agttatgaaa aaactatttt
       61 taattatgat gattggaaca atcgtaactg catgcaccgg taagtctgaa aaatcggaca
      121 atcctttttt ctcggattat aaaaacgagt acaaggcacc gcctttcgaa agtatcaaaa
      181 atgcgcacta tctgcctgct tttgaagaag gcatcaaaca acatcaggca gaaatcgatt
      241 caattgcctc caatcccgaa gctccgactt ttgccaacac cattgaagca atggaataca
      301 gcggcgagtt gctcagcaag gtcagtgccg tattttcaaa tcttcagtcg gccgaaacaa
      361 acgatgaaat ggattctatt gccaaaatcg taaccccgaa acttaccgaa cacagcgata
      421 atatctacct gaacgacaaa ctgtttcaac gggtaagagc cattttcgac accaaagcca
      481 cgctgggact tacacccgaa caggaccgtg tgctggagaa aatctacaaa cgattcatac
      541 gcagtggcgc tgccctcaat gaagaacaaa aagcacagtt gcgagaaatc aacaaggaac
      601 tgagcacact cgaattgact ttcggacaga atatcctggc cgaaaccaat gcgttcaaaa
      661 aatttgtgac caacaaagaa gaattgaaag gacttcctga aggcatcatc gaggcagctg
      721 ccgaagaagc taaaaaagaa ggaaaagaag gtcaatggct tttcacaacc tctaagacaa
      781 gtttcatacc cgtgcttcag tacggtgaga accgcgaact gcgcaaagag ctgcttatgg
      841 cttacgtgga ccgctgtaat cacgataacc aaaacgacaa caagccggtg attaataaaa
      901 taatgaaact cagggtgaga aaagctcaat tattgggctt cgagagtcct gcagcgttta
      961 ttcttgacaa caccatggcc aaaactcccg atgcggttta cagcttcctg aacaaagtat
     1021 ggacgccggc agtagccaaa gcaaaggctg aagcatccga actgcagaaa ctgatggacg
     1081 cagaaggtaa aggcgaaaaa ctcgaaccgt gggactggtg gttctatacc gaaaaactgc
     1141 gcaaagcaaa atttgatttg gacgaagaac agttgaggcc ctacttcaag cttgaaaatg
     1201 tgcgaaaagg tgtgttcgat ctgaccacaa aactattcgg actgaagtat gaaaaactgg
     1261 agggaatgcc cgtttatcac aaggacgtag aagtatttaa agtaaccgat gccgacggaa
     1321 aactgattgg aattctttat accgactatt tcccgcgtgc gggtaaaacg gtgggtgcct
     1381 ggatgagcaa tttccaggaa cagtatatca aagacggtgt gaatcagcgt ccgataatcg
     1441 taaatgtggg taatttcacc aaacccacag ccgataaacc ttcgctgctt accatggacg
     1501 aagtggaaac actcttccac gaattcggac acgcgttgca cggactgctc gcaaactcta
     1561 cctatccgag tctttcgggc accaatgtag cacgcgactt tgtggaattg ccctcgcaaa
     1621 tcatggaaaa ctggtgctgg gaaccagaag tgatgaaaac ctatgctctt cactacaaaa
     1681 ccggcgaagt gatgcctcag gaactgatgg ataaaatcag aaaagccgga acatttaatc
     1741 agggatttgt gaataccgaa ttgctgtcgg cttctatcct ggatatggat taccacgtga
     1801 aaaaagatac ctccgatatc gatgtgaacg cgtttgaaaa agcaagcatg gataaaatgg
     1861 gcatgatacc tcagattata gtgcgctaca agagcccgat tttcaaacat atttttgaag
     1921 gcggatactc tgccggatac tatagctaca cctgggcagc agtgctcgac gccgatgctt
     1981 atcaggcttt caaggaaacc ggcgatattt ataacaagga agtggctact gctttccgta
     2041 aaaacatcct cgaaaaaggt gacagcgaag aaccgatgaa actttaccgc acgttccgcg
     2101 gagccgatcc gaatccggac gcattgctta ttaagcgcgg tctgaaatag gtttttctgt
     2161 gccctgctat gtgaatatga agagagacgt tgcttgcagc gtctcttttt ttgtgcaatt
     2221 tctgctgagg aaatgattta acttcatata aaatgtgtaa ttttgttttc tcaacggata
     2281 atccgatatt ccagtcatga aatcagacct ccagaaactc attgttccct ctttgccgct
     2341 cactgcgctt atctacgtgg tcagttttgt tatctggatg ttattttata ttatctacaa
     2401 atatccttac cctatgcccg gcaagatagg gagtatagcc gaaagttttc tttcgtcgca
     2461 gtcgctggca gcttacctgt tgagttacgc catcgtggtg gttaattcaa tggttatagc
     2521 gcagatgaac aacaggtttt cattcatccg tacgcgcact tttctgccca cattcgttta
     2581 tctgttggtg tcggtttgct ggctgccctt acatggcaat tacgtagcta atctggctgc
     2641 tttttttgta ttgctggcta tttttatgag tcttgtcatg tataagatgc cgaacggcgt
     2701 ggagcaggca tttctgtcat tttttctgct ggcactcagc acgtttctgg tgcctgacta
     2761 cgcttatctg attgtgctct tttggatggg ttattttttt ctgaaatgtt tttcacccag
     2821 ggtgtttttt gcttctgtat tcggttttgt cactccctgg attttgtatc tgagtgtcat
     2881 tttctttatt ttcaacgaac agggatttgt gtccgacatc ccggcttttt tctaccggta
     2941 ttctgttttt cactacgaga atattccagc tattatttat gtccttttga tgattcttat
     3001 cgttgctata tcacttgtac agatgattgc caaatcccgt caggatagca ttcaggcccg
     3061 caatgaactg aattttctca aattgctgtt tttcggtgta gccatgcttt ttgtcctgcg
     3121 tttttcaaat tatatatcct acttgccgct catagcgatg tttttctccg tttttactgc
     3181 atatactttt acccttatca gaaatctgtt taatttcatt gttttcatcg cactggtcgc
     3241 attgagtttt ttattcgcat tttatatcat cattatctga tttttttatg tttgactttg
     3301 cagagtgggg gtatctcggg ctttttatag catcttttct ggcggctacc gttgtaccgt
     3361 tcagttcaga ggtggttttt tcggccatgg ttttcggtgg gctccatccg tggacgtgtg
     3421 tactggtggc cactgcaggc aactggttgg gaggtatgac aagtttttac atcggtcggt
     3481 taggcaaaat agagtgggtg gagaagttcc ttcatatcaa aaaagaaaaa gtagagagat
     3541 ttgttgaaaa attcagccgc tacggcgatt ggtttgtgtt tttctcattc gtcccgtttg
     3601 tgggtgatgt gatagccgtt gctgccggat ttttccgagg ccgctggtgg gtggtagcca
     3661 tagccatgtt gttgggtaaa tttatccgct acgtcatctg gatgtatgcc aatgggatgt
     3721 ttatgtagta tttcgtcaca catttttcat tttattgtat aaatttcgtc attgttttgt
     3781 aacacagtcg gtttaatttt gcatcagtta ataacgaaat atttatcaaa atgaaaaaaa
     3841 tatttttctt cgccattttc tctctggcta tcatcagtct gaatgctgca aataaggtaa
     3901 aagcagaagc agatgcaaaa gcaaacgccg aagtaagcgt acaaaaaaat gcaatcaacg
     3961 gacgggttat tgacgctgtc acaaatgaga cattagcagg tgctgagatt tcagtcaacg
     4021 ggcaaaaaat ctattcggac ctggatggaa atttcgacat caaagatgcc aaaggtgaaa
     4081 aaatcaaaat aaccatcagt atgatttctt acgcagatca ggtggttgaa attgaaccgg
     4141 gtacgcataa tcttctcaat gtgaaactga aacaacttta atgtaaaaaa atacatacgc
     4201 tttatttgaa tcgcaccgga ttttccggtg cgattttttt ttcgcgtttg tcacggtttc
     4261 ttccccgatt ttcaaaaatc aaatgattta gttatatttg tactccctaa aaaaatgaaa
     4321 tactaataaa aaatcaattc attatgatca aaaaaacttc agtatttatc gtgcttgcta
     4381 catttctggt gctgaatatg tattcacaaa accg
//
LOCUS       DAORKP010000169         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5061_length_4351_cov_9.266527, whole genome shotgun sequence.
ACCESSION   DAORKP010000169 DAORKP010000000
VERSION     DAORKP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5061_length_4351_cov_9.266527"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLN75_08370"
     CDS             <1..596
                     /locus_tag="PLN75_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HRG04170.1"
                     /translation="NYEEKVPSMDAWPVWNYTDGQLIRVVCYTNAPKARLLLNGKEVG
                     QIQSYNENTGIIFWDIPYENGTLSVQGLDKNDKEISHYQIKSSKQPYALQITSADKSV
                     SKDQVAHVTVQVVDENGIPVMLSDNEITCRIVGDGKLLGLEASNNEDMSDYNDNKHRV
                     FHGRIMAYIKPVKEEGNIRIKFTSPWLESAETEIQIK"
     gene            complement(684..1568)
                     /gene="deoC"
                     /locus_tag="PLN75_08375"
     CDS             complement(684..1568)
                     /gene="deoC"
                     /locus_tag="PLN75_08375"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRG04171.1"
                     /translation="MATKFEELFSTYGCDLITDELVKKELEKILSEHFEENNTTDVYK
                     QCFNCIDLTSLKETDTEDEIRTMVQKVNDFEENFPNTPNVGAICVFPSMVSTVKSTLT
                     EPIGIAAVSAGFPASQTFLEVKVAETSLCVMEGATEIDVVISVGKMLEGKYEEVYEEL
                     TEIKSACRDAHLKVILETGALKTASVIKKASVLAMAAGADFIKTSTGKIPVAATPEAT
                     YIMCSAIREWNEKTGEKVGYKPAGGISTTEDAVKHYTLVKEILGGQWLNNKLFRFGAS
                     RLANNLLTSITGKEVNYF"
     gene            complement(1673..2014)
                     /locus_tag="PLN75_08380"
     CDS             complement(1673..2014)
                     /locus_tag="PLN75_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRG04172.1"
                     /translation="MTLEEAQKSVDEWIKTYGVRYFGEMTNMAILTEEVGELARIMAR
                     TYGDQSFKESDRNYNLADEMADVLWVLICMANQTGVNLTEALEDNIRKKTERDKTRHL
                     ENIKLEPPKHT"
     gene            complement(2019..2687)
                     /locus_tag="PLN75_08385"
     CDS             complement(2019..2687)
                     /locus_tag="PLN75_08385"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRG04173.1"
                     /translation="MKYIKIHKEGKWIIAFTLFALLAVNVIIYLQFPKPILAVNIVGS
                     AFLFIFVMFFFRNPRRVVEIDDPSLIVAPADGTVVVIEPTEEHEYFGDKRMQISIFMS
                     VFNVHANWYPVAGKVLKVAHHDGNHRVAYSPKASIDNERSTIVIETQGKTRILVRQIA
                     GAIARRIVTYAHPGIKCHLNEHIGFIKFGSRVDVFLPLDAEIFVTLGESTTGNETIIA
                     RLNE"
     gene            2860..4203
                     /gene="purB"
                     /locus_tag="PLN75_08390"
     CDS             2860..4203
                     /gene="purB"
                     /locus_tag="PLN75_08390"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRG04174.1"
                     /translation="MSLTSLSAVSPVDGRYHSKTSVYSAYFSEFALIRYRVLVEVEYF
                     IALCQLPLKQLETVPFAVFPKLREIYENFSENDASRVREIEKVTNHDVKAVEYFLKEK
                     FDQLNLNDNKEFIHFGLTSQDINNTAVPLSIKKALEEVYLPEMELIVKELSYKADEWR
                     DVSMLAKTHGQPASPTRLGKEIMVYVSRLNQQLELLKSVPQTAKFGGATGNFNAHHVA
                     YPAIDWRAFGNKFVEEKLGLRREPWTTQISNYDNLAALFDALKRINTILIDFSRDIWM
                     YVSMEYFKQKIKPGEVGSSAMPHKVNPIDFENAEGNLGVANAIFGFLAEKLPVSRLQR
                     DLTDSTVLRNAGVPMAHTIIATQSLLKGLDKLLLNETAVYKELDNTWAVVAEGIQTIL
                     RREKYPEPYEALKALTRTNEGINERTIRDFIETLRVSENIKDELKRITPRNYTGI"
BASE COUNT         1199 a          948 c          952 g         1252 t
ORIGIN      
        1 gcaattacga ggaaaaagtt ccctcgatgg atgcctggcc tgtttggaat tataccgacg
       61 gtcagttaat ccgcgtggtt tgttacacta atgccccgaa agcccggctg ctgctgaatg
      121 gaaaagaagt tggccaaatt cagtcttata atgaaaatac cggaataatc ttttgggata
      181 ttccttatga aaatggaacg ttgtcggttc agggtttgga taaaaacgat aaagaaatca
      241 gccattacca gattaaatcg tcgaaacaac cttatgcact gcaaattact tctgccgaca
      301 aatccgtttc taaagatcaa gtggctcatg taacggttca ggtggtggat gaaaacggta
      361 ttccggtgat gctgtctgat aatgaaatta cttgcaggat tgtgggtgac ggcaaactgc
      421 tggggctcga agcaagtaat aacgaagata tgagcgatta caacgacaac aaacaccggg
      481 tatttcatgg ccggatcatg gcttatatca aaccggtaaa agaggaagga aatatcagga
      541 taaaatttac atcaccctgg cttgaaagtg ccgaaacgga aattcagata aaataaatcc
      601 gcacgaaaat cggaaatata taaagggctg tttcaaaaaa aataaagaaa cagcccttta
      661 gttgagaaat gaatttcttc cacttagaag tagttaactt ccttgcctgt aatgcttgtc
      721 agcaggttgt tggccagccg gctggctccg aagcggaaca gtttgttgtt gagccactgc
      781 ccgccgagta tttctttcac caacgtataa tgtttcacag catcttcggt agtcgatatg
      841 ccgcctgcag gtttgtatcc aaccttttct cctgtttttt cgttccactc ccggatggcc
      901 gaacacatga tgtaagtggc ttcgggagta gcggctaccg gtattttgcc ggtggaggtt
      961 ttaataaagt cggcaccggc tgccatggcg agcactgaag cctttttaat gacagaggct
     1021 gttttgagtg cgcctgtctc cagtatcact ttcaaatgag catcccgaca ggctgatttt
     1081 atttctgtca gttcttcgta tacttcttca tatttgcctt ccagcatttt accaaccgaa
     1141 attaccacgt caatttcggt ggcgccttcc atcacacaaa gcgaggtttc ggcaactttt
     1201 acttccagaa aggtttgcga agcgggaaat cctgccgaaa cggctgcaat cccaatgggt
     1261 tcggtcaggg ttgatttcac agttgacacc atggatggaa acacgcagat ggctccaaca
     1321 tttggagtgt tcgggaaatt ttcttcaaaa tcgttcactt tctgaaccat tgttcttatt
     1381 tcatcttccg tatcggtttc cttcagcgat gtgaggtcga tgcagttgaa acactgtttg
     1441 taaacatcgg ttgtattgtt ttcttcgaaa tgttcggaaa gtattttttc cagttctttc
     1501 tttactagct catcggtaat caaatcgcac ccgtaagtgc tgaaaagttc ttcaaatttt
     1561 gttgccataa gtatatttac aatttacgat ttacgattta cgaattgtta acttctaatt
     1621 tctaatttca aatttctaac ttcaaaagtc ccccggcagg gacctgcatg cctcaggtat
     1681 gtttaggggg ctccagtttt atgttttcga ggtgtcgggt tttatctctt tcggttttct
     1741 tgcggatgtt atcttccagg gcttcggtca gattcacgcc ggtctgatta gccatgcaaa
     1801 tgagtaccca cagtacatca gccatttcat cggccaggtt gtaattcctg tccgattcct
     1861 tgaacgactg atcgccatac gtgcgtgcca tgatgcgtgc cagttcaccc acttcttcgg
     1921 tcagaatggc catgttggtc atttcgccga aataacggac tccgtaggtt tttatccact
     1981 cgtccacgct tttttgggcc tcttccagtg tcattttttt attcgttcag tcgggctatg
     2041 attgtttcat tgccggtggt tgattcgccc agtgttacaa aaatttctgc atcgaggggt
     2101 agaaatacat ctactcttga accgaatttg ataaatccga tgtgttcatt caggtggcat
     2161 ttgatgcccg gatgcgcgta agtgactatc cgtcgggcaa ttgctccggc tatctgacga
     2221 accagaatgc gggttttgcc ttgtgtttca attacaatgg ttgagcgttc gttgtcgata
     2281 cttgctttcg gcgaatacgc aacacgatga ttcccgtcgt gatgggccac tttcagtact
     2341 tttccggcta cgggatacca gttggcgtga acattaaaaa cagacatgaa aatggaaatc
     2401 tgcatccgtt tgtcgccgaa atattcgtgt tcttctgttg gctctattac cactacagtg
     2461 ccatcagccg gtgcaactat caggctcgga tcatcaattt cgaccactcg gcgcggatta
     2521 cgaaaaaaaa acatcacgaa aataaaaagg aacgccgaac caacaatatt caccgctaaa
     2581 attggtttgg gaaactgcaa atagataatt acattgactg ccagcagagc aaaaagtgta
     2641 aaagcaataa tccacttgcc ttctttatgt atttttatgt atttcataaa tgctaatatc
     2701 ctttcccgct tcgagcgtgg attttttttt aattaacaaa aatagttatt ttaatctgat
     2761 accacaagtg gacggcttaa attttcattt atctcaaaaa tgattaactt tgcaacctgt
     2821 tttttcagga aaaaatttac aaatatgatt aacccttgta tgtcactaac ctcactaagt
     2881 gcagtttcgc cggttgacgg gcgctatcat tctaaaacaa gtgtttattc ggcctatttt
     2941 tcggaatttg cactcattcg ctaccgggtg ctggtggaag tggagtactt tatcgctctc
     3001 tgccaattgc ctcttaaaca gctggaaacg gttccttttg cagtattccc gaaattgaga
     3061 gagatttatg aaaatttctc ggaaaacgat gcttcaagag ttcgggaaat agagaaagta
     3121 accaatcacg atgtaaaagc agtggaatat ttcctgaaag aaaaatttga tcagctgaat
     3181 ttgaacgaca ataaggaatt tatccatttt ggtctcactt cgcaggatat aaacaatact
     3241 gctgtgccgc tatccatcaa aaaagcactc gaagaagttt acctgcccga aatggaactc
     3301 atagtgaaag agctaagcta caaagccgat gaatggcgcg atgtgtcgat gctcgccaaa
     3361 acacacggcc agccggcatc gccaacgcgg ctcggaaaag aaataatggt gtatgtgagc
     3421 agactgaatc aacagctcga actacttaaa tcggtgcctc aaacggccaa attcggaggt
     3481 gctaccggca atttcaacgc ccatcatgtg gcttatccgg ctatcgactg gcgggcgttt
     3541 ggcaataaat tcgtggaaga aaaactcggt ctccggcgcg aaccgtggac aacccagatt
     3601 tccaactacg ataacctggc ggctcttttt gatgcattaa aacggattaa caccattctg
     3661 attgacttca gccgcgatat ctggatgtac gtttccatgg agtatttcaa acaaaaaatc
     3721 aaacccggag aggtgggctc gtcggcaatg cctcataaag tgaatccgat tgactttgag
     3781 aatgccgaag gaaatcttgg tgtggcaaat gccattttcg gctttctggc agaaaaactg
     3841 cccgtatcgc gcctgcagcg cgacctcact gacagcactg tgctgcgcaa cgcgggagta
     3901 ccgatggctc ataccatcat tgccacccaa agcctgctga aaggattgga taaattgctg
     3961 ctcaacgaaa ctgccgttta taaagagctg gacaatacct gggcagtggt ggctgaaggg
     4021 attcaaacca tactccgacg cgagaaatac cccgaaccct acgaagcact taaagcactg
     4081 acccgtacca acgagggcat caacgaacgt acaatccgtg attttatcga aacactccgg
     4141 gtaagtgaaa atataaaaga tgagttgaag cggattacac ccagaaatta cacgggaatc
     4201 tgataccgat tttttaattt gtttgaaaat gtttttttcg ttaattctcc ctgtgtcggg
     4261 aataatgtga ctttatgttt gattttttaa aactgatcag gcgattcatt ccgccttaca
     4321 agaaatatgt ttttctgagc ttgctgtcca a
//
LOCUS       DAORKP010000170         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5062_length_4351_cov_8.647114, whole genome shotgun sequence.
ACCESSION   DAORKP010000170 DAORKP010000000
VERSION     DAORKP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_4351_cov_8.647114"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="PLN75_08395"
     CDS             <1..113
                     /locus_tag="PLN75_08395"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRG04175.1"
                     /translation="QMRGVQKQHSITTTSDFTGVFQQAKTREEFLNLIKG"
     gene            complement(303..950)
                     /locus_tag="PLN75_08400"
     CDS             complement(303..950)
                     /locus_tag="PLN75_08400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG04176.1"
                     /translation="MNAEIVSVHYKTPDYIYEQYASVRKFYPFLPYRIIDGSDDGKIY
                     FRDLEEKDTNFHVERLGYNIHHGPGMDHAIKNSASDYLLILDSDVTLKGDVMTPMFEE
                     FAGYAVGRKRIVNALGMQNRDISWIGRLFPKTFKYTYIHPLCMLISKQAYLQFKPFFK
                     HGAPCIDAMIDIHKKNQTHMLAEFPVEEYVNVKYQGTVKEWGMNIPKWKYLIPRF"
     gene            complement(1040..3367)
                     /locus_tag="PLN75_08405"
     CDS             complement(1040..3367)
                     /locus_tag="PLN75_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG04177.1"
                     /translation="MKKILILSVIVVFCGLAASGQVAMGKWQTHFAYNIVEHVAQSEN
                     KVFAVSAGKLFSVDKRDGKMDLYSKITGLNGINISKIGYDDTNKTLLIVYQDGNIDFL
                     SAGGVKNLPDYYNKQMSADKSINHILIANNKAFLSCNFGIITLNMLKMEIQDTYYIGN
                     NASEVKVLNTTIHNGNIYALSASNIYFASASEPHLINYEYWKTMSNLPGNGNFQALHS
                     FGNFLVLLRDGKLYKQDSNGIWSNIETTTVFNKIIVSGDYMQAYSNSDTYIYDKQFAQ
                     KQITGITDVKDGSYDNTKNCFWFAAGDQGLAEYQINGTAPVINHFKPIGPAVNIPYRM
                     YFAGERLFVVPGEKFLGPTNRPGYVMILENNTWKNISHDEIEKSLNGLRVEDFSTIAI
                     DPDDNKHFFVNSASTGIYEFRNDEFYMHYDRSNSNIETALNIPNDYFQWTDNVILDKD
                     KNLWMTNDLTSHGIKVFKSNGEWAKLEYSGVNNKQSLGKILISNQNPNHKWVISRRHF
                     PGICIFDDNGTLDTQTDDKTSFFSSLNYNTSDGVKSINPLLYFCIEQEKNGAIWVGTN
                     EGPLLFNNPSKVFEPGFTCSRIIIPRNDGTGLGDYLLEDQKVTAIAIDGANRKWIGTE
                     SSGVYLMSENGQETILHFTSKNSPLLSEQILSIAIHPVTGEVFFGTGGGLLSYQSDAA
                     EAGNTFSNVHVYPNPVRENFTGLITITGLVENTQVKITDVAGNLVSETTSKGSIATWD
                     ARNSFGQKVSTGVYLAICISPDGLQTSTAKILIIN"
     gene            complement(3431..4021)
                     /locus_tag="PLN75_08410"
     CDS             complement(3431..4021)
                     /locus_tag="PLN75_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRG04178.1"
                     /translation="MRKFVFATNNLHKLEEVRAILKDKATIVSLAEAGCTDEIPETAE
                     TLDENAALKAKYVFEKFGFECFADDTGLEIGCLNGKPGVYSARYAGEPSDSIKNMQKV
                     LQEMEGEKNRSAQFRTVICLVEDGRFRYFEGIIKGRIAEAPRGNAGFGYDPIFIPDGY
                     TKSFAELSSETKNTISHRALAVKKLTDYLENANGEI"
BASE COUNT         1103 a          904 c          909 g         1435 t
ORIGIN      
        1 tgcagatgcg cggcgtgcag aaacagcatt ccatcactac cacatccgat tttaccggtg
       61 tattccagca agccaaaacg agagaggaat ttttgaattt gataaagggg tagcgtacag
      121 cttatctatt ctgataggaa aacaaaaaag taatcattct tccatcccat gatgtttctc
      181 cgttctttta gaattttgca ctgcttcact ttctgaatct tctgcagata aatcggagga
      241 gtttcttaaa tacgagttct tctgtaacaa ggtatttcga aaaatccagc taaatctttt
      301 tattaaaaac gtgggatcag atacttccat ttcggaatgt tcattcccca ttctttcacg
      361 gtgccctggt attttacatt cacatattct tctaccggaa actcagccag catgtgagtc
      421 tgattttttt tatgaatatc aatcatggca tcaatacagg gtgctccgtg tttgaaaaaa
      481 ggtttgaatt gcaggtaagc ctgtttgctt atcagcatgc agagcggatg aatgtaggtg
      541 tatttaaatg ttttcgggaa aagtctgcct atccaggaaa tatcccggtt ttgcattccc
      601 aaagcattga caattcgctt tctccctact gcatatcccg cgaattcttc aaacattggt
      661 gtcattacat ctcctttcag ggtgacatcg ctgtccagaa tcagcaggta atcagaagca
      721 gaatttttaa tggcatggtc cattcccggc ccatgatgaa tattgtaacc caaacgttct
      781 acatgaaaat ttgtatcttt ttcttccaga tcccggaagt aaattttacc atcgtcacta
      841 ccgtcgataa tccgataggg aaggaatgga taaaatttac gaacactggc atattgttcg
      901 taaatgtagt ccggagtctt gtaatgtaca gatactattt ctgcattcat gtggtttgat
      961 ttccggatag atagtattta ttcgggaagg atgtgattat tcccttcagt tgtacgctac
     1021 ttatccttat tttcatgttt tagttgataa taagtatctt ggcagtagag gtttgcaacc
     1081 cgtccggtga tatacagata gccagataaa cgcctgtgct cactttctgt ccgaatgaat
     1141 tcctggcatc ccaggtagca atgcttcctt tggaggttgt ctcactgaca agatttccag
     1201 ccacgtctgt tattttcacc tgcgtattct ccaccagtcc ggtgattgtg ataagtccgg
     1261 taaagttttc cctcaccgga tttggataaa catgtacatt gctgaatgtg tttccggctt
     1321 ctgctgcatc gctctgatag gagagcagcc ctcctccggt accaaaaaaa acttcgccgg
     1381 taaccggatg tattgcaatg gataaaatct gttccgaaag caggggactg tttttggagg
     1441 tgaaatgaag gatggtttcc tgcccgtttt ctgacatcag atatactcct gatgattcgg
     1501 ttccaatcca ctttctgttt gctccgtcaa tggctatggc agtcactttt tgatcttcca
     1561 gcaggtaatc gcccagtcct gtcccgtcgt ttcggggtat gatgattctc gaacaggtaa
     1621 atccgggctc gaacaccttg gatgggttgt tgaaaagcag aggtccttcg ttggtaccca
     1681 cccagattgc tccgtttttc tcctgttcga tacagaagta gagcagggga ttgattgatt
     1741 tcaccccatc gcttgtgttg taatttaatg atgagaaaaa agatgttttg tcgtccgtct
     1801 gtgtatcgag agtaccgttg tcgtcaaaaa tgcatattcc cggaaaatgc cttctggaaa
     1861 ttacccattt atggtttgga ttttggtttg aaataaggat tttccccagg gattgtttgt
     1921 tattcacgcc actgtattcc agttttgccc actcaccgtt ggatttgaat acttttattc
     1981 cgtgtgaagt caggtcgttg gtcatccaca ggtttttatc cttgtcgagt attacgttgt
     2041 cggtccactg aaagtagtcg ttgggtatgt tcagagctgt ttcgatgttg ctgttgctgc
     2101 ggtcgtagtg catgtagaat tcatcgttcc tgaattcgta aattcctgta cttgcactgt
     2161 ttacaaaaaa atgtttgttg tcatcgggat caatggctat ggttgaaaaa tcctcgactc
     2221 tcaagccgtt gagcgatttc tcaatctcat cgtgcgagat gtttttccac gtgttattct
     2281 ccagaatcat cacatatccc ggtctattag taggcccgag aaatttttca ccgggcacca
     2341 cgaagagtct ttctcctgca aaatacattc tgtagggtat gtttacggca ggccctatgg
     2401 gtttgaaatg atttatcaca ggggcagtac cgtttatctg gtattcggcc agtccctgat
     2461 cgcctgcagc aaaccagaaa cagtttttcg tattgtcata gctgccgtcc tttacgtcgg
     2521 tgatgccggt aatctgtttt tgtgcaaatt gtttgtcgta aatataggta tcggagtttg
     2581 aataggcctg catgtaatcg cccgaaacaa tgattttatt aaaaacagtt gttgtttcta
     2641 tgtttgacca aatgccgttt gaatcctgtt tatagagttt tccgtcgcgg agcagtacca
     2701 gaaaattgcc aaatgaatgt aacgcctgga aatttccgtt gccgggcaga ttgctcattg
     2761 ttttccagta ttcatagttg atgagatgcg gttccgaagc tgatgcaaaa taaatattcg
     2821 aggcagacag ggcataaata ttaccgttgt ggatagtggt gttcagcact tttacctccg
     2881 aagcgttgtt gccgatgtaa taggtatcct gtatttccat tttcagcata ttgagcgtaa
     2941 taatgccaaa attacaggag agaaaggctt tgttattggc tatgagtatg tgattgatgc
     3001 tcttatctgc actcatttgt ttgttgtagt aatcgggcag gtttttcact ccgcctgccg
     3061 aaagaaaatc tatatttcca tcctgataga ctatcagcag cgttttattt gtatcgtcgt
     3121 atccgatttt ggagatattt ataccgttta aaccggttat cttactgtat aaatccattt
     3181 tcccgtctct tttgtcgaca gaaaaaagtt tgcctgcact tacagcaaaa actttatttt
     3241 ccgactgagc cacatgttct acaatgttat atgcaaagtg agtttgccac ttacccattg
     3301 ccacctgccc actagcagcc agaccgcaaa aaacaactat aacggatagg atgagaattt
     3361 ttttcataag aatattatag agatgcattg aaaaaacaga ttttcggggt ttttattgtt
     3421 ttcgcaccgg ttatatttca ccattggcat tttcaagata gtcagtcagt ttttttacgg
     3481 caagcgcacg gtggctgatg gtgttttttg tttctgaact cagttctgca aaacttttgg
     3541 tgtaaccgtc gggtataaaa atcggatcgt atccgaaacc ggcgtttcct ctgggtgcct
     3601 ctgcaatgcg gcctttgatt attccttcga aataccggaa acggccatcc tccaccaggc
     3661 agattaccgt tcggaattgt gcactgcggt tcttctcgcc ttccatttcc tgcaatactt
     3721 tttgcatgtt ttttatcgaa tcggaaggct ctccggcata acgtgcggaa tacacgcccg
     3781 gtttcccgtt caggcatccg atttccaaac cggtatcatc ggcaaaacat tcaaatccga
     3841 acttctcaaa tacgtatttt gccttcaggg cggcattttc atccagcgtt tcggctgttt
     3901 cgggtatctc atcggtgcat cctgcttcag ccagactcac tatcgttgcc ttgtctttga
     3961 gtatggcacg cacttcttcc agtttgtgaa gattgttggt ggcaaataca aattttctca
     4021 ttccttttcc ggtttcagat ttttttttga aaaacaaagg taaaattttt cggacagatt
     4081 ccgaaatact tcatcgaatt attcccgata atgctgctga gttggttttt tttttgataa
     4141 aatggaatat tcagctattt ttgcaaaccg aaacaagagg atgtatttca gaaaataaac
     4201 gcgaatcact gagtatgctg tacattgcag gcaagtctgt cttacatagt caggattatg
     4261 tttttttttt tgaaaaattg agaaacaatg tatttttatg aaacagccat taaacacaaa
     4321 ttttcaccca tgagaatgaa gtcccgatag c
//
LOCUS       DAORKP010000171         4309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5157_length_4309_cov_7.500235, whole genome shotgun sequence.
ACCESSION   DAORKP010000171 DAORKP010000000
VERSION     DAORKP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_4309_cov_7.500235"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2541
                     /gene="priA"
                     /locus_tag="PLN75_08415"
     CDS             82..2541
                     /gene="priA"
                     /locus_tag="PLN75_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRG04179.1"
                     /translation="MMKYIDVILPLPLGNTFTYSVPDEWAGSVCTGMRVVVPFGRKKM
                     YTGIVSVIHSMAPTLYEAKEIICLLDEHPVVRFPQLKFWEWMSAYYMAYPGEVYQAAI
                     PAGLKLESETQVRINPAYEEETTLTEKEYKIIDALSDGKIHTVSDLNKVTEMRDTLPL
                     LKKLMEKGLVEVSEELTEKYRPKTENYIRLTSEAASEEELRRIFDELSRSRKQLELLM
                     KYIELSKCLSKNNIVEVSKKELLEKSGCSPAVLNGLTGRNVLEIYQKETGRLDRSEVL
                     TEEKHPLNEYQQTALEEINRQFTDRKTVLLHGITSSGKTELYIHLIEKTLAEKKQVLY
                     LVPEIALTTQLTSRLKRIFGNKLAVYHSRFSDAERVEIWNSLLHDKGCEVVIGARSAL
                     FVPFRKLGLVIVDEEHETSYKQFDPAPRYNARNAAIVLASLHGANTLLGSATPAIESY
                     FNAKTGKYGYVALNQRYREMELPDILIADTKEAYRKKQMEGHFTPLLLEKIRKALQNN
                     EQIILFQNRRGYAPYLECRGCAYVPKCKNCDVSLTVHKFLNKLTCHYCGYTEHIPELC
                     PVCNTPQLEHKGFGTEKIEQEIEEFFPQAHVSRMDVDTTRNKKSYDKIITDFEQGKVD
                     ILVGTQMVTKGLDFERVSLVGILNADNMLNFPDFRSHERAFQLMAQVSGRAGRKNKRG
                     TVVLQTSAPEHPVIGQVLRNDYAAMFSTQCTERGQFGYPPYTRLIEITLRHRDVKTTE
                     RAAQELAAELRKVFGKRVFGPNIPPVSRIQNLYIKNILLKIETASSTEKAKEILKQIT
                     GQLLTREPYKSLKISVDVDPV"
     gene            2861..4309
                     /locus_tag="PLN75_08420"
     CDS             2861..4309
                     /locus_tag="PLN75_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRG04180.1"
                     /translation="MQEIKFYSGENIPLELHKTRIVQKLHLVPIERRLEALYEGGFNT
                     FRLSTLDVFLDMLTDSGTNAMSDNQLSAMMQADDAYAGSQSFFRLQKAVEDVLGKKYY
                     LPVHQGRAAENILSKAYIKSGNLIPMNYHFTTAMAHITENGGKIVELLYDEAYVVNSS
                     NPFKGNMNIEKLEEMIRLHEPKNIPFIRMEASTNLIGGQPFSVKNLRDVRALADKYGI
                     RLVLDASLLGENAYLVMKREEEFKNSTLGEIIRVMTDLSDLVYFSARKLSSSRGGGIC
                     TNDIGIYKELEALVPLYEGFLTYGGISVREIESMAVGLYETLDETVICQSPNFIEYLV
                     NELDKKGIPVIKPAGVLGVHLDAMQVCSHIPQSQYPAGALAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELVRLAVPRRVFTLSQIKYVEDRVNWLFDNRELIGGLKFVYEP
                     PVLRFFMGGLEPVNDWPQRLMAKFKEDFGESL"
BASE COUNT         1235 a         1142 c         1021 g          911 t
ORIGIN      
        1 catgaagact tgaagattat ttagcaaaac tttcaaacat tcaaacaccc aaacatccaa
       61 aaattcaaac attcaaacaa tttgatgaaa tacattgacg tcatacttcc gctgccactg
      121 ggcaacacgt tcacctattc cgttccggat gagtgggcgg gttcggtgtg caccggcatg
      181 cgtgtggtag tcccttttgg caggaagaaa atgtacacgg gcatcgtcag cgttatccac
      241 agcatggctc ccaccctcta cgaggccaaa gaaatcatct gcctgctgga tgagcatccg
      301 gtcgtccgtt ttccgcagct taaattctgg gaatggatga gtgcatacta catggcgtat
      361 ccgggcgaag tctatcaggc tgccatacct gccgggctga agctggagag cgaaactcag
      421 gtgcgcatca atccggcata cgaggaagaa acgacactga ccgaaaaaga atataaaatc
      481 atcgatgccc tgtcggacgg aaaaattcat acggtatcgg acctgaataa ggtcactgag
      541 atgagagaca cattacccct actgaaaaaa ctgatggaaa aagggctggt ggaagtgagc
      601 gaagaactga ccgaaaaata ccgtccgaaa accgaaaact acatccggct cacctccgaa
      661 gcagcatccg aagaggaact ccgacgaatt ttcgacgagc tgagccgttc gcggaaacag
      721 ctggaactgc tgatgaaata catcgaactg tccaaatgcc tgtccaaaaa caacatcgtg
      781 gaagtttcaa agaaagaact gctcgaaaag tcgggttgct cgcctgccgt gctcaacggc
      841 ctgaccggac ggaatgtgct ggagatctat cagaaagaaa ccggacggct cgaccgctcg
      901 gaagtgctca cggaagaaaa acatccgctc aacgaatatc agcagacagc gctcgaagaa
      961 atcaaccggc agtttaccga caggaaaaca gtgttgcttc acggcattac gtcgagcggc
     1021 aagaccgaac tctacatcca cctgatagaa aaaaccctgg ccgaaaagaa gcaggtgctt
     1081 tacctggtac ctgaaattgc actgaccaca cagctcacct cccgcctgaa acgtattttc
     1141 ggcaataaac ttgccgtgta tcattcccgc ttctcggatg ccgagcgggt ggaaatttgg
     1201 aactccctgc tgcacgacaa gggctgtgaa gtagtgatag gggcacgttc ggcgcttttc
     1261 gtaccatttc gtaaactggg actggtcatt gtggacgaag agcacgaaac aagttacaag
     1321 cagtttgatc ctgcgccgcg atacaacgcc cgcaatgcag ccatcgtgct ggcttcgctc
     1381 cacggtgcca acaccctgct cggcagtgcc actccggcca tcgaaagcta tttcaacgcc
     1441 aaaaccggaa aatacggcta cgtggctctc aatcagcgtt accgcgagat ggaactgccc
     1501 gatattctga tagccgatac caaagaagca taccgcaaaa aacagatgga aggtcatttc
     1561 actcccctgc tgctcgaaaa aatccgaaaa gcactgcaaa acaacgagca gatcatcctg
     1621 tttcagaacc ggcgcggata tgcaccctac ctggagtgca ggggctgcgc gtatgttccg
     1681 aaatgcaaaa actgcgacgt aagcctcacg gtacataaat ttctgaacaa actcacctgc
     1741 cactactgcg gctacacgga gcacatcccc gaactctgcc ccgtttgcaa cacgccgcaa
     1801 ctcgagcaca agggattcgg tactgaaaaa atcgaacagg agatcgaaga gttttttcct
     1861 caggcacacg tatcacgcat ggatgtggac actacccgca ataaaaaatc gtacgacaaa
     1921 atcatcaccg actttgaaca gggaaaggtg gatatcctgg tgggcactca gatggtaacc
     1981 aaaggacttg actttgagcg ggtgagtctg gtaggcattc tgaatgccga caacatgctc
     2041 aattttcccg atttccgctc gcacgaacgg gcattccagc tcatggcgca ggtaagcggc
     2101 cgcgcgggac gcaagaacaa gcgcggcacc gtggtactgc aaacctccgc accggagcat
     2161 ccggtcatcg ggcaggtact gcgcaacgat tatgcagcca tgttcagcac acaatgcacc
     2221 gagcggggtc agtttggcta cccgccctac acccgcctga tagaaatcac gctgcgtcac
     2281 cgcgacgtaa aaaccaccga acgggcagca caggagcttg ccgccgagct tcggaaagtg
     2341 ttcggcaagc gggtgttcgg accgaatata ccacctgtat cacgcattca gaatctgtat
     2401 ataaaaaaca tcctgctcaa gatagaaacc gcttcctcca ccgaaaaagc gaaagaaatc
     2461 ctgaaacaaa tcaccggaca gctcctcacc cgcgagcctt ataaatcatt gaaaataagt
     2521 gtggatgtag atcctgtatg atttataaat aattctcgat tgaaaaatta caattggttt
     2581 catgttccgt gttccgtgtt ccgtgttccg tcaatatctg attgctgaaa gcttttttta
     2641 ctaaaatcat acattttctt accttttaat gcattatttt tcaaatttca gtatctttgc
     2701 acgcataaat tcggttttga gaacaatttt gtttatttcg ggcttacaga taatacattt
     2761 tgttattact gtctgtacgg aataaactcc tcagacacta tttataaaaa aaacacacaa
     2821 acatacacac ttcgttattt cgacaaaaaa acgcaacaac atgcaggaaa ttaaatttta
     2881 cagcggagaa aacattccgc ttgagttaca caaaacacgt attgtacaaa aactccacct
     2941 cgtacccatc gaacggcggc tcgaagcgct ttacgaaggg ggattcaaca ctttccgcct
     3001 gagtacgctg gatgtatttc tggatatgct caccgacagc ggcaccaacg ccatgagcga
     3061 caatcagctt tcggccatga tgcaggccga cgatgcctac gccggttcgc agagcttttt
     3121 caggcttcag aaagccgtgg aagatgtgct gggcaaaaaa tattacctcc ccgtgcatca
     3181 gggacgcgct gctgagaaca tcctctccaa agcatacatc aaaagcggca acctcatacc
     3241 gatgaattac cacttcacca ccgcaatggc acacattacc gaaaatggcg gtaaaatagt
     3301 ggaactgctc tacgacgaag cttacgtggt gaactcttcg aatccgttca aagggaatat
     3361 gaacatcgaa aagttggagg aaatgatcag actccacgag ccgaaaaaca tccctttcat
     3421 ccgcatggaa gcctctacca accttatcgg cggacagcca ttctcggtga agaacctgcg
     3481 tgacgtacgc gcactggccg acaaatacgg aatccgcctg gtgctcgacg ccagcttgct
     3541 gggcgaaaac gcttacctgg tgatgaaacg cgaggaagaa ttcaaaaaca gcacgctcgg
     3601 cgagattatc agggtgatga ccgacctgtc cgacctggtg tatttctcgg cgcgcaaact
     3661 gagttcatcg cgcggaggcg gcatctgcac caacgacatc ggcatctaca aggaactgga
     3721 agccctcgtt ccgctttacg aaggatttct cacctacggc ggcatctcgg tacgcgaaat
     3781 cgaaagcatg gcagtaggac tttacgagac actggatgaa accgtaatct gccagagccc
     3841 gaacttcatc gaatacctgg taaacgaact cgacaagaaa ggcattccgg taatcaaacc
     3901 tgccggtgtg ctgggtgttc acctggatgc catgcaggta tgttcacaca ttccgcagtc
     3961 gcagtatccc gccggggcgc tggcagcggc gttgttcctt atttcaggta tccgcggcat
     4021 ggagcgcggc tctatctcca accagcgcga cgaatacggc aacgaaacct acgccgacat
     4081 ggaactggtt cgcctggctg taccgcgccg tgtattcacc ctctcgcaga tcaaatacgt
     4141 ggaagaccgc gtaaactggc tcttcgacaa ccgcgaactg ataggcggac tgaaatttgt
     4201 gtacgaaccg cccgtgctcc gcttcttcat gggaggactc gaaccggtga acgactggcc
     4261 tcaacgcctg atggctaaat tcaaggaaga tttcggcgaa agcctgtaa
//
LOCUS       DAORKP010000172         4248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5310_length_4248_cov_9.406392, whole genome shotgun sequence.
ACCESSION   DAORKP010000172 DAORKP010000000
VERSION     DAORKP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_4248_cov_9.406392"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="PLN75_08425"
     CDS             complement(<1..1616)
                     /locus_tag="PLN75_08425"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG04181.1"
                     /translation="MRLYHKLFLYFFAMIFILLGTITFVQYQRERSSHADQLDGLLDT
                     YNVVVHNFIQNKNFSRDELEQIVRLFPDSNLRITVIDTTGIVLYDSSVKESDRLENHK
                     NRPEFIMAAQSGKGKSLRRSATTGREYYYYALKFPDYYVRSALPYTLELDSLLKTNMF
                     FFYFMLVILLLAVIALYFITRNFTKSIDRLRKFTERITDGEEPDMEVDFPKDELGEIS
                     SNMVTLYRQLAKTKNAVNNEREKLINHLYISQEGLGIFSSEKKEILTNTTFIQYTSFL
                     TDKNEATSDEIFNLAEFAEINQFIDENLSSNQMRRKRMTIEKSSRYFFVQCIVFQDKT
                     FEISISDVTAQEHENELKRHLTQNISHELKTPVSTIMGYMESILENPELSPERQRFFV
                     QRSFQQAQRLTALLQDISTLNMLDEKRKIYENETCNLYVIVEEVLNDLHLEIEERRFE
                     VVSNIGKEVVILGNSLLLYSIFRNLMDNALTYAGEQVKVEINCYRQDDRFYYFTFSDN
                     GIGVSEEHLNRLFERFYRVDKGRSRKMGGTG"
     gene            complement(1705..2391)
                     /locus_tag="PLN75_08430"
     CDS             complement(1705..2391)
                     /locus_tag="PLN75_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRG04182.1"
                     /translation="MNPYKILVVDDEEDLCEILQFNLETEGYQVDVAYSAEEALIKDI
                     SQYNLLLLDVMMGEMSGFKMARLLKDNPKSKNIPVIFLTAKDTENDRITGFNIGADDY
                     ISKPFSIREVLARVKAVLRRMETSTGQASVNELRHENLVMNLNNKKVTLDGSEISFTK
                     KEFEILKLLLENKNRVFTREEILSRVWSDEVIVLDRTIDVNITRLRKKIGKYGKQIIT
                     RLGYGYCFEE"
     gene            complement(2650..>4248)
                     /locus_tag="PLN75_08435"
     CDS             complement(2650..>4248)
                     /locus_tag="PLN75_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HRG04183.1"
                     /translation="TTFGIRTIEVRPNDGFYLNGKKTVFQGVCNHHDLGPLGAAVNDA
                     AIRRQVRIMKDMGVNAIRTSHNMPAPELVRACDEMGMMLMVETFDEWKMPKVANGYNL
                     FWNEWAEKDMVNMLHHYRNNPSVVMWCVGNEVPEQGNGDMGPKRNRFLQDICHREDPT
                     RPVTNGMDNPNAVTGNNFAALLDVPGFNYRPHLYHENYKKLPQQIVLGSETASTVSSR
                     GVYKFPVERKSMAQYDDHQSSSYDVEHCSWSNLPEDDFIQHEDLPYAIGEFVWTGFDY
                     LGEPTPYYTNWPSHSSLFGIVDLAGIPKDRFYLYRSHWNKDEKTLHILPHWNWKGREG
                     EVTPVFVYTSYPSAELFINGVSQGKRTKDLSIKRDGSDNDMAKKNFDRQKRYRLMWME
                     TKYQPGTVKVVAYDENGKAVAEKEMKTAGKPYRIVLEADRSTITADGKDISFVNVKIV
                     DKDGNFCPTDEREISFNVKGSGIFKAVANGNPASLESFQAPKMKLFSGQLTALVQSLE
                     KTGSITLEATAKGVKSGKITLEAK"
BASE COUNT         1059 a          972 c          933 g         1284 t
ORIGIN      
        1 cccgtgccgc ccatcttgcg gcttcgaccc ttgtccacgc ggtaaaaacg ctcaaaaaga
       61 cggttcaggt gttcttctga cactccgatg ccattatcgg aaaatgtaaa gtagtaaaac
      121 cgatcatcct gacggtagca attaatctcc acttttacct gctcaccggc gtatgtaaga
      181 gcgttgtcca tcagattcct gaagatggag tacagcaaca atgaattgcc gagaatgaca
      241 acctctttac cgatattact caccacttca aaccgtcttt cttcaatttc caggtgcaaa
      301 tcattcagca cttcctccac aataacatac agattgcagg tttcgttttc gtaaattttc
      361 cgcttctcgt cgagcatgtt gagtgtggag atgtcctgca gcaatgctgt gaggcgctgt
      421 gcctgctgga acgacctctg aacaaagaat ctctgccttt cgggggacag ctccggattt
      481 tcgaggatac tttccatgta gcccatgatg gtgcttacgg gtgttttgag ttcgtgggag
      541 atgttttgcg tcaggtgccg tttcagctcg ttttcgtgct cctgagcagt tacgtcgctg
      601 atggatattt caaatgtttt atcctgaaaa acaatacatt gcacaaagaa atagcggctg
      661 cttttttcaa tggtcattcg tttgcggcgc atctgattgc tgctcagatt ttcgtcaata
      721 aactgattga tttcggcaaa ttcggccagg ttgaaaatct catccgacgt agcctcattc
      781 ttatcggtga gaaaactggt gtactgaatg aaagtcgtat tagtgagtat ttcctttttt
      841 tccgatgaaa aaattccgag tccctcctgt gagatataaa ggtgattgat cagtttttcg
      901 cgttcgttgt ttactgcatt ttttgttttg gccaattgcc ggtaaagcgt caccatattg
      961 ctgcttattt cgcccaactc atcttttgga aaatccactt ccatatccgg ttcttcgccg
     1021 tcggtaatcc gttcggtaaa tttgcgcaag cggtcgatgg atttagtaaa atttcgggtg
     1081 atgaaataaa gcgctattac agccagcagc aggatgacca gcatgaaata gaaaaaaaac
     1141 atattcgttt tcagaagtga atcgagttcc agggtatacg gcaatgcact cctgacgtag
     1201 taatccggga atttaagggc gtaatagtag tattccctgc cggtggtggc cgaccgtcgg
     1261 agcgatttgc ctttgccact ctgcgcagcc atgataaatt cgggtcggtt tttgtggttt
     1321 tcgagtcggt cgctctcttt taccgaagaa tcgtaaagca caatgcctgt agtatctatg
     1381 actgtaattc gcagattgga atccgggaaa agccgtacaa tctgctccag ttcatccctg
     1441 ctaaaatttt tattctgaat gaaattatga acaaccacat tgtaagtatc cagcagcccg
     1501 tcgagctggt cggcatgcga acttcgctcc cgctgatact gcacgaaagt aatggtgcca
     1561 agcaggataa aaatcattgc aaaaaaatag agaaaaagct tgtggtaaag acgcatggat
     1621 tagtttttat gaaatgcagt tgactttaaa tttctttaca gaatgattga ctgcggttgt
     1681 tttcaggtaa atccgtcaga aaaatcactc ctcgaaacaa tagccataac caagacgggt
     1741 aataatttgc ttcccgtatt tcccgatttt ttttcgcaga cgggtgatat tcacatctat
     1801 agtccggtcc agcacgatta cctcatcaga ccataccctg gagagtattt cttcccgggt
     1861 aaaaacacgg tttttgtttt cgagcagcag tttgagtatc tcaaattcct ttttggtgaa
     1921 tgaaatttct gacccgtcga gcgttacttt tttattattc agattcatca ccagattttc
     1981 atgcctcagt tcgttgaccg atgcctgtcc ggtactggtt tccatacgcc gcaaaacggc
     2041 tttaacgcgt gcaagcacct cacggataga aaatggtttc gaaatgtaat catcggcacc
     2101 gatattgaaa ccggtaatac gatcgttttc ggtatctttg gccgtaagaa atatgaccgg
     2161 gatatttttg gatttaggat tatctttcaa caaccgggcc attttgaatc ccgacatttc
     2221 gcccatcatc acatcgagca gcaacaagtt atactgtgag atgtctttta tgagggcttc
     2281 ttcggctgag taagccacat ccacctgata accttcggtt tccagattaa actgaaggat
     2341 ttcacacagg tcttcttcgt cgtcgacgac taaaattttg taaggattca cgttttgaag
     2401 ttagaagtta gaagttagaa atcagaagct tgtctgcctc tggcaggcag gcaaatcaga
     2461 aataagtcaa cagtgaattt gcctgatttt tcatataaat ttcactgcaa agtaactttc
     2521 tttatatttc gatgacatgt aatagatatt acaaatgtat gacaaaaaac cggaatccgg
     2581 attttcttta aacaaaaaaa acagcagtca gaaaccgctg ttttttttta ctgtttaatt
     2641 attacagaat tactttgctt cgagagtaat tttcccactt ttcacacctt tggcagtggc
     2701 ttccagtgta atgctgcctg ttttttcgag actctgcacc agggcggtaa gttgtccgct
     2761 gaagagtttc atcttcggag cctgaaagct ttcgagactg gccggatttc cgtttgctac
     2821 agctttgaag atacccgaac cttttacatt gaagctgatt tcgcgctcat cggtcgggca
     2881 gaaattcccg tccttatcta ctattttcac attcacgaat gagatatctt ttccgtcggc
     2941 tgtaatggtg cttctgtcgg cttccagtac aatgcggtag ggtttccctg ctgttttcat
     3001 ttctttttca gccaccgctt tgccgttttc atcataagcc accactttca ccgttccggg
     3061 ttgatatttg gtttccatcc acatcaggcg ataccgtttc tgtcggtcga agttcttttt
     3121 agccatatca ttatcacttc cgtcacgttt gatgctcagg tctttggtac gttttccctg
     3181 acttactccg ttgataaaaa gttcggccga aggataactg gtataaacaa ataccggcgt
     3241 aacctctcct tcgcgtccct tccagttcca atgaggcagg atgtgcaggg ttttctcgtc
     3301 cttgttccag tggctgcgat acaggtagaa ccgatccttg ggaataccgg ctaaatcgac
     3361 tataccgaac agagaactat ggctgggcca gttggtataa tacggtgtgg gttcacccag
     3421 ataatcgaag cctgtccata caaattcgcc aatagcgtaa ggcaaatctt cgtgctgaat
     3481 gaaatcatct tcggggagat tggaccagct gcaatgttcc acatcgtagg atgacgactg
     3541 atgatcgtcg tactgagcca ttgatttgcg ttcaacgggg aatttatata caccccgcga
     3601 acttaccgtg gatgcagttt cgctgccaag tacaatctgc tggggtaatt tcttgtaatt
     3661 ctcatggtat aaatgagggc gatagttgaa cccgggcacg tcgagcaacg ctgcaaaatt
     3721 attgccggtc acggcgttcg gattgtccat tccgttggtt acggggcgtg ttggatcttc
     3781 gcggtggcat atatcctgca gaaaacggtt gcgtttcggg cccatatctc catttccctg
     3841 ctcgggcact tcatttccca cacaccacat taccaccgag ggattattac ggtagtgatg
     3901 caacatattt accatgtctt tttcggccca ttcgttccag aaaagattgt atccgttggc
     3961 tactttcggc attttccact cgtcgaaagt ttccaccatg agcatcatcc ccatttcgtc
     4021 gcaggcgcga accagctcgg gtgcgggcat gttgtgcgaa gtacggatgg cattcacacc
     4081 catatctttc ataatgcgta cctgacgacg gatggccgca tcgttcactg cagctccgag
     4141 aggtcccaga tcgtggtgat tacacactcc ctgaaatacc gttttctttc cattgagata
     4201 aaatccgtcg ttgggtcgaa cttcgatggt gcggatgccg aaagtagt
//
LOCUS       DAORKP010000173         4187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5483_length_4187_cov_8.544773, whole genome shotgun sequence.
ACCESSION   DAORKP010000173 DAORKP010000000
VERSION     DAORKP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_4187_cov_8.544773"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1361)
                     /gene="mtaB"
                     /locus_tag="PLN75_08440"
     CDS             complement(27..1361)
                     /gene="mtaB"
                     /locus_tag="PLN75_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRG04184.1"
                     /translation="MIDNSLFTNKKVVYHTLGCKLNFAETSAIGKQLADEGFVKARAG
                     EEADICIINTCSVTDTADHKCRQAISRLHRLHPKAKMIVTGCYAQLKPQEIAGIEGVD
                     LVLGANEKFDILSYLENGNSSSEPKIRRGDILKVKEFKPSVSHDDRTRYFLKIQDGCD
                     YYCSFCTIPLARGHSRSGTIAETVKMAEEVISQGAREIVLTGVNTGEFGKRNGEKFMD
                     LLKALDEIDAEVRFRISSVEPNLLTEEIIRFVSVSKRIMPHFHIPLQSGSNEVLKLMK
                     RRYTTEDFADKVAIIRQYLPDAFIGVDTIVGVRGETDELFEESVQFIRSLDFSQLHVF
                     TYSERADTKMLEIEHNVAIKERKRRSDLLHVISDEKTKSFYEKQIGKRATVLWESKRD
                     GDVMHGFTENYIRVERPYDRKMVNVVENIIPGEWNADKTALISPLESPSRGS"
     gene            1674..3611
                     /locus_tag="PLN75_08445"
     CDS             1674..3611
                     /locus_tag="PLN75_08445"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HRG04185.1"
                     /translation="MKNIFLFLFLSSFSVLSAQSNVTIPNIQGSGNSSSYSGQQVATS
                     GIVTAVYIGGGMINGFFIQDAAGDGNSATSDGVFVYSSSKSVEPGDLVQLTARVSEYN
                     NRTQLSDVTAFQVLSKNNTLPAVKIVYDIYNWDWEKYEGMLVEFQQTLYVNNTYNLQQ
                     YGELELGIRRKPSPTNLALPASSEYSALVSENALRPVYIDDAYSSYTYPVIFADENGT
                     RRTGERVDRLQAVVDYVNSKYVVYPARFPVQFYGNPRKTTHDDIGNYNLKVCGFNLEY
                     YLTTPNSSGMGPSNQTELERQHTKIVDAMLAIDADVYGLVEIEQGQQALTKLSQALNA
                     ASSGKSFSFVNDGGSVNGTYTKAAYLYRSDKVTPYKSLKSINSEGPANRKKLQAFTLK
                     SNNERFMFGINHFKAKSGCSSASGADADKNDGQSCYNATRVDEAKAVIAAINSNKAFY
                     DDQDALVMGDLNAYAMEDPIQAFVQAGFTDLHRQFHADSAYSYVYRGEAGYLDHALAN
                     ESMTRQITGVTAFHINADEPSIFEYSGSAYQPNMFRSSDHDPVVVGIRLGDNVNVNSL
                     PFEEKVQILPTVVDTYFRVQHAENAYIQVFSLNGIKLFQDVVRSADQEFSVSSLGLIP
                     GAYVVRLLGENTIVSRIIFVK"
     gene            3686..3767
                     /locus_tag="PLN75_08450"
     tRNA            3686..3767
                     /locus_tag="PLN75_08450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3720..3722,aa:Leu,seq:tag)
BASE COUNT         1070 a          979 c          966 g         1172 t
ORIGIN      
        1 aatttctaat ttctaatttc taacttctaa cttccccggg agggggattc aagggggctt
       61 ataagtgccg ttttatctgc gttccattct cccgggatta tattctctac cacgttcacc
      121 atttttctgt catacggtcg ttccacacgg atgtaattct ctgtaaatcc gtgcattaca
      181 tctccgtcgc gtttggattc ccaaagcacg gtcgcccgtt tcccgatttg tttttcgtaa
      241 aacgatttgg ttttttcatc cgaaatgaca tgaagcaaat cgcttcggcg tttacgctct
      301 tttatagcca cattgtgttc tatttcaagc attttagtgt cggcgcgttc ggagtaggtg
      361 aatacatgca attgcgagaa atcaagcgac cgaatgaatt gtacactttc ttcgaaaagt
      421 tcgtccgttt cgccccttac gcccactatg gtatccacgc cgataaaagc atcgggcaga
      481 tattgtctga taatggccac tttatcggca aaatcttcgg tggtataacg tcttttcatc
      541 aatttgagaa cctcgttgct gccactttgg agcggaatgt gaaaatgagg cattatccgc
      601 ttgcttaccg atacaaaccg gatgatttct tcggtgagca gattaggttc caccgacgaa
      661 atccggaaac gtacttcggc atcgatttca tcgagggctt ttagcaggtc cataaatttt
      721 tcgccgttgc gttttccgaa ttctcccgta ttcactccgg taagcactat ttcacgggct
      781 ccctgcgaaa tgacttcctc tgccattttt actgtttcgg ctatggttcc gctccggctg
      841 tgtcctcgcg caagcggaat ggtgcagaag gagcagtaat aatcgcagcc gtcctgaatt
      901 ttcagaaaat aacgggtgcg gtcgtcgtgc gaaaccgaag gtttgaactc tttcactttc
      961 agaatatctc cgcgcctgat tttaggttcg gatgagctgt ttccattttc caggtagctc
     1021 aggatgtcaa atttttcgtt tgcaccgagc accaggtcca ctccttcgat gccggcaatt
     1081 tcctgcggtt tgagctgagc gtaacaaccc gtcacaatca ttttagcttt cggatgcagg
     1141 cggtgaagcc ggctgattgc ctggcggcac ttgtgatcgg ccgtatctgt taccgaacag
     1201 gtgttgatga tgcagatatc ggcttcctcg cccgcacggg ctttcacgaa accttcgtcg
     1261 gcaagttgtt tcccgatggc agaggtctct gcaaagttca gcttgcagcc aagcgtatgg
     1321 taaactactt ttttattggt aaacagggag ttatcaatca tttttttttc aacatgtaaa
     1381 aatacagaat aatcgttgtc cgcttaatga cacaaaatcc attgccaggc cttttaaggc
     1441 ttggattcaa tggtttaaga gattgactta agccaaagtt ttttataagt tgggctaaag
     1501 tgcttacatc tgttttctaa atgcccacgc ccgaaagagg agtggcagct aaaaatgtgt
     1561 caggacgttt gttttcaggc tacaaagtta caaaaaaata gaactaaacc gaaatgaaaa
     1621 atatttcgcg gtttttattt atctttgcag gttccaagtc aaattttttt tcaatgaaga
     1681 atattttcct ttttttgttt ttatcctcat tttcagtcct ttcagctcag tcgaacgtca
     1741 ctataccgaa tatccaggga agcggcaact ccagcagcta cagcgggcag caggttgcca
     1801 cttccggaat agtcacagca gtgtatatcg gcggcggtat gattaatggt ttttttattc
     1861 aggatgctgc aggcgatggc aattccgcca catccgacgg tgtatttgtg tactcttcgt
     1921 ccaaaagtgt ggaacccggc gatctggtac agcttacagc tcgcgtaagt gagtacaaca
     1981 accgcactca gttatcggat gttaccgcat ttcaggtatt gagtaaaaac aatacattgc
     2041 cggctgtgaa aatcgtttat gatatttaca actgggactg ggagaaatac gaaggaatgc
     2101 tggttgaatt tcagcagacg ctctatgtga ataataccta caacctccag caatacggcg
     2161 aactggaact gggcatccgc cggaaaccct caccgacaaa cctggcttta ccggcctcat
     2221 cggaatactc ggccctggtc agtgaaaatg ccctgcgtcc cgtttacata gacgatgcat
     2281 acagttcgta cacctatccc gtgatttttg ccgacgaaaa cggcactcgc cgaacgggcg
     2341 aacgagttga ccggttgcag gctgtggtcg attatgttaa ttcaaaatat gtcgtgtatc
     2401 ctgcccgatt tcccgttcag ttttatggaa atcccagaaa aaccacgcac gacgatatcg
     2461 gaaactacaa cctgaaagtg tgtggtttca acctggaata ttatcttacc acacccaatt
     2521 catccggcat gggaccctcc aaccaaaccg agttggagag gcagcacacc aaaattgtgg
     2581 atgcaatgct tgctattgat gccgatgtgt atggcctggt ggagatcgaa cagggacagc
     2641 aggctctcac caaactatcg caggctctca atgctgcatc ttcgggaaaa agtttttcgt
     2701 ttgtcaacga tggcggctcc gtgaacggca cctacaccaa agccgcatac ctgtatcgat
     2761 ccgataaggt aactccctat aaatcattaa aaagcatcaa ttccgaagga cctgccaacc
     2821 ggaaaaaact tcaggctttt acgctgaaaa gtaacaacga acggtttatg ttcggtatca
     2881 atcatttcaa ggcaaaaagc ggctgttcgt cggcctctgg cgctgatgcc gacaagaacg
     2941 acgggcaaag ctgctacaac gctacccgcg tggacgaagc aaaagccgta attgccgcca
     3001 tcaactccaa taaagccttt tacgacgacc aggatgctct ggtgatgggc gacctcaatg
     3061 cctatgccat ggaagacccg attcaggcat ttgtacaggc cggttttacc gacctgcaca
     3121 gacaattcca tgccgacagt gcctacagct atgtgtatcg tggtgaagcc ggctacctgg
     3181 atcacgccct ggcaaatgaa agtatgaccc gccagattac aggagtgact gcctttcata
     3241 tcaatgccga cgaaccgtcc atctttgaat acagcggttc ggcctatcag cccaacatgt
     3301 ttcgctcgtc cgaccacgat ccggtagtgg tgggaatccg gctgggtgat aatgtaaatg
     3361 tgaattctct gccgtttgag gaaaaggtac agattttacc caccgtagtg gatacctact
     3421 tcagggtaca gcatgccgaa aatgcgtaca ttcaggtatt ctcgctgaat ggcatcaaac
     3481 tctttcagga cgttgtcaga tcggccgatc aagaattctc agtcagcagt ttaggattaa
     3541 ttccgggagc gtatgtagtc cgtctgctgg gtgaaaacac cattgtgagc cggattatat
     3601 tcgtgaaata atcgccgtat tgtttgaaca gtacgaaaaa agattgtatc tttgcacccg
     3661 ttattcaaaa aatgatacac cacatgcggc tgtggcgtaa ttggtagccg cgccagactt
     3721 aggatctggt gccgtaaggc gtgggggttc gagtcccttc agccgcacag aaaagtggat
     3781 gtcttaaaat aagacatcca ctttcttttt gcggtgaaca ttacggtcag cagtcaggaa
     3841 cgtctctttg gagaaggtgt ttgttttatt aataatgtcg cacctacggt gcttttgttg
     3901 gttggtacta ttggttttct accaaaatgt cgcaccgttg gtgcttaaga taaagctccg
     3961 ataggggcga tattatggta gcattgaatt tcaatccgaa atacagagtt cctttacgag
     4021 cggtattagg attttcattc tggtggtaat agaattccga ttctctcaga ttaatgagga
     4081 gctattatga gcaagttctc tgcctgcggc agacaggtct tgcctgcggt aggcacctgc
     4141 cagtgacatg aacgtttcta tttcatcaaa gaaacgttca aaagtct
//
LOCUS       DAORKP010000174         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5528_length_4172_cov_10.190187, whole genome shotgun sequence.
ACCESSION   DAORKP010000174 DAORKP010000000
VERSION     DAORKP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_4172_cov_10.190187"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /gene="dut"
                     /locus_tag="PLN75_08455"
     CDS             complement(<1..266)
                     /gene="dut"
                     /locus_tag="PLN75_08455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRG04186.1"
                     /translation="MKIKIVNESGHPLPEYATLHAAGMDLRAHLSTPVELKPLERKLI
                     PTGLYIELPVGYEAQIRPRSGLALKKGITLLNSPGTIDADYRGE"
     gene            647..1753
                     /locus_tag="PLN75_08460"
     CDS             647..1753
                     /locus_tag="PLN75_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRG04187.1"
                     /translation="MGALATIIWATGSFWIPLMFGLVFLILPMPDIAVLRSYYKTKYL
                     MSFAYLLFAVVSFYFLVTNSGFQFNKTIRVSTPSVAILQIIIFTYVNITLINPSFLKF
                     RKLLRPLIPAFILCIAYFFAFTNDPGGKLYQVMYYSLFGFFAVLLVIYSFMFYRHYKL
                     FRLRMNNYYSEESSSHLLWVAKSQIFVTITGILAFFAGFIPTGYMGYFLAVMILFFAY
                     YAIRYIYYANSFSVVEAVLDVNQEKEIKNNSVDTAHSQLDEAISKWEIEKKYLERNLT
                     VENVAISLCTNRTYLSNFINTQKNKTFNEWINGLRINEARQLLLNNPDMPILEVCELV
                     GFSDKSNFGRHFTKITSYSPAAWRKEHSNPNNKS"
     gene            1750..3573
                     /locus_tag="PLN75_08465"
     CDS             1750..3573
                     /locus_tag="PLN75_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRG04188.1"
                     /translation="MKPKSFFLLNISLALSAAGWLFTACSDSEQIHSQKLGKAIDLFY
                     LENKNDSVLQLLDELPAEKLSFSEIQLAAIFRAAAVCEAGKPDSAMTVLKQIEPGKLH
                     KDTRYYYESILGLIEFRQFLSEKAYNTLVTLTNKETPDIRAEALAQRTIGRIMISYGE
                     DKSAIDWLMRSRQNFEKAGLEKSVAINDKFLGNISIRLNVYREALPYLNAAISTLKKY
                     NDGAELFYAYVVMIEYFIRQNQPDSARYYTQKAQQSFDVKRDKTMQALLYNNRGEIEK
                     MNGNYEGAIEMFDSTLLLGTDYYYSSVRRQTAYLNLAEVYNKMKLPDMARKNALDALN
                     TVEVNQKYNIRYAVYYQLAKSYAPADVTRIQEYMDSAIYCLQKYHASQSVDLINFFDT
                     QQELHLSQHKIEELKSKAVKIRLNYLIGGFFLLAVITALTVLFHIQKDRNRALRMLVH
                     KNLKLLEKEVDEERLARQKRSNTSKRKCQTENDEEKLQTIYDCFIHWLYDEGNYMRND
                     ISLSVASKELNTNREYLSRAINEHNKHFNDLVNNYRVKEIIRIFSNPGDDRNKLTLQV
                     LASEVGFNSNSVFIDAFRKVTGMTPTQFREHLNENQPKPKG"
     gene            3853..>4172
                     /locus_tag="PLN75_08470"
     CDS             3853..>4172
                     /locus_tag="PLN75_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04189.1"
                     /translation="MKTICTQVSRTMILGILFVCLAAVSATGQVAARYSDYVKTNADS
                     RLISLATEIHPAIYLKGNTDMKSFGNGDATVVNTDAASIPMLYATNPAFGKVELIKIT
                     IKSAT"
BASE COUNT         1278 a          881 c          825 g         1188 t
ORIGIN      
        1 atttctccgc ggtaatcggc atcgatggta ccgggtgagt tcagtaaggt aattcccttt
       61 ttcagagcaa ggccgcttcg cggtcgtatc tgagcttcat aacctaccgg taactcgatg
      121 tataagcctg tgggtatcaa ttttcgttcc aaaggtttga gctctacggg tgtactgagg
      181 tgcgcgcgca ggtccatgcc ggcagcgtgg agcgtggcgt attcgggcag tggatgtccc
      241 gattcattta ctatttttat tttcatgctg tttgcttatc ggttatttgt atttttaact
      301 tgcaaaaata cgattttaat cggtatggtt aaaagaatca accattaaaa tatctaatat
      361 taagtgtata aagttagcta aattccccga aaaaaagcag gatgaaaaga cgataagaat
      421 attctccctg aaaatttgtg aatattttca cttttttaaa caatttaatc gtacatttgc
      481 acattgaggg aaagattaat tccggctttt tctttgagca ttaaatactt ttctgtaaaa
      541 atgtgactct ttcagaacga agtggtacac gcatttttaa ggtatttcat atttttccct
      601 gcgtttttga ataaatcaat tcaaaaagaa agattctgca ataattatgg gagctttagc
      661 aactataata tgggcaacgg ggagtttctg gattcctctg atgttcgggc tggtgtttct
      721 gatacttccc atgcccgaca ttgctgtact gaggtcttac tacaaaacca aatacctgat
      781 gagctttgct tatctgttat ttgccgtagt atctttctat tttctggtca ccaattccgg
      841 gtttcagttt aataaaacga tccgggtttc cactccgtcg gtagctatcc tgcaaatcat
      901 tattttcacg tacgtcaaca ttacgctgat taatccgtca ttcctgaaat tcagaaaact
      961 gttacgtccg ctgattccgg cattcattct gtgcattgct tatttttttg catttacaaa
     1021 tgatccggga ggtaaacttt accaggtaat gtattactca ttgtttggat tctttgctgt
     1081 tttacttgtt atctacagtt tcatgtttta ccggcattac aagctattca gacttcggat
     1141 gaacaactat tactccgaag aatcatccag tcacctgctc tgggtggcaa aatctcaaat
     1201 atttgttact attaccggta tccttgcttt ctttgccgga tttattccta caggctatat
     1261 gggatatttc ctggctgtca tgattctgtt tttcgcatat tacgctatcc gttacattta
     1321 ttatgctaat tcattttcgg tagtagaagc agttctggac gtgaatcagg aaaaagaaat
     1381 taaaaataat tctgtagaca ctgctcattc gcaactggat gaagcaataa gcaaatggga
     1441 aatagagaag aaatacctgg agagaaatct tacggtggaa aacgtggcta tttcgctctg
     1501 tacaaaccgc acgtatcttt cgaatttcat caatacgcaa aagaataaaa cattcaacga
     1561 gtggataaac ggacttcgta tcaacgaagc caggcaactg ctgttgaaca atcccgacat
     1621 gcccatcctg gaagtttgcg aactggtagg attctctgac aaaagcaatt tcggaaggca
     1681 tttcactaaa attaccagct actcgcctgc agcctggcgc aaagaacatt caaacccaaa
     1741 caataaatca tgaaacctaa atcctttttt ttactaaaca tatcgcttgc cctctcggca
     1801 gcgggatggt tatttacagc ttgttcggac agcgaacaaa tacattctca aaaactcgga
     1861 aaggcaatcg acctgtttta ccttgagaat aagaacgaca gcgtactgca gctacttgat
     1921 gaacttccgg ctgaaaagct atctttttcg gaaatacagc ttgctgctat cttcagagca
     1981 gcagcagtat gcgaagccgg gaaaccggac agcgccatga ccgtactcaa acagatagag
     2041 cccggcaaac tccacaaaga cacaaggtat tactatgaaa gtattctcgg gctgattgaa
     2101 tttcgtcagt ttctgtcaga aaaggcatat aatacactcg taacactcac caacaaagaa
     2161 actccggata tccgcgcaga agcacttgca caacgcacga ttggtagaat aatgatcagt
     2221 tacggcgaag ataaaagcgc gatagactgg ctgatgcggt cgcgtcaaaa cttcgaaaaa
     2281 gcaggactgg agaaaagtgt ggccatcaat gataaatttc tgggaaacat aagcatccgc
     2341 ctcaatgtgt acagggaagc actgccctac ctcaatgcag ctatatcaac attaaagaaa
     2401 tacaacgacg gcgctgagct tttctatgct tacgtggtga tgattgagta tttcatcagg
     2461 caaaaccagc cggattctgc ccgatactat actcaaaaag cccaacaatc tttcgatgtg
     2521 aagcgagata aaacgatgca ggcattgctg tacaacaacc gcggtgaaat tgaaaaaatg
     2581 aacggaaact atgaaggagc catcgaaatg tttgactcca ccttgctgct gggtacggat
     2641 tattattact ccagtgtcag gagacaaact gcttatctca atctggccga agtgtataac
     2701 aagatgaagc tacccgacat ggccagaaaa aacgcactgg atgctctcaa taccgttgaa
     2761 gtgaatcaaa aatacaacat caggtacgct gtttactatc agcttgcaaa atcgtatgct
     2821 ccagctgatg ttacccggat acaggaatac atggattctg ccatttattg tcttcaaaaa
     2881 tatcacgctt cgcagtcggt agatttgatt aattttttcg acacacagca ggaactccac
     2941 ttgtcgcagc acaaaatcga agagttaaaa tcaaaagccg tcaaaatacg gttaaattac
     3001 ctgattggcg gtttctttct gctggctgtc attacggcac tgacagtgtt atttcatata
     3061 cagaaagacc gaaacagggc actccggatg ctggtacaca agaacctgaa actcctcgaa
     3121 aaagaagttg atgaggaaag gttagcacgg cagaagcgca gcaacacatc aaagagaaaa
     3181 tgtcaaaccg aaaacgacga agaaaaactt caaactattt atgactgttt tattcactgg
     3241 ctgtatgacg aaggtaatta catgcgcaat gacatctcgc tgagcgtagc ttccaaagag
     3301 ctcaacacca accgtgaata cctttcaaga gctataaacg agcataacaa gcatttcaac
     3361 gacctggtaa acaattatcg tgtgaaggaa attatccgga tattctcaaa ccccggtgac
     3421 gaccgaaaca aactcacgct gcaggtgctc gcctccgaag tgggcttcaa ctccaattcg
     3481 gtatttatcg atgcgttccg caaagtgacc ggtatgacac cgacacagtt cagagaacac
     3541 ctcaacgaaa atcagcccaa gccgaaaggg taaatcgaat gctcaacatc cgaaagtctt
     3601 ttcggattgt tcaattcaaa aatatttccc ccgtttttgt ctctttttgg tcaaatcgta
     3661 aatttatttt tacgatttga ccattctttt tttattgtag tcattatatt tgtacatgtt
     3721 gaaaaccggt ttaattcatt ttatccaaat ttatcgaaaa gtagtataaa acagtatcaa
     3781 accgatagtg aatactaata aaaaacaata accaataacc tatttttttt taacttacat
     3841 aaatgaacca caatgaaaac aatttgtacc caagtaagca ggaccatgat tctgggaatt
     3901 ctgtttgttt gtttggcggc tgtttccgct accggtcagg ttgctgcccg ctacagcgat
     3961 tatgtgaaaa caaatgccga ttcccgtctt atttcactgg ctactgaaat tcatccggct
     4021 atttacctga aaggtaatac ggatatgaaa agtttcggaa acggcgatgc aacagtagtt
     4081 aataccgacg cggcttccat tccgatgctt tatgcaacta atcctgcttt cggcaaggtg
     4141 gaactgatta aaattactat caagagcgcc ac
//
LOCUS       DAORKP010000175         4158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5572_length_4158_cov_9.128686, whole genome shotgun sequence.
ACCESSION   DAORKP010000175 DAORKP010000000
VERSION     DAORKP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5572_length_4158_cov_9.128686"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLN75_08475"
     CDS             <1..314
                     /locus_tag="PLN75_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRG04190.1"
                     /translation="ELSTANNIRVVLCSVLPAYDYPWRKGLEPNIKIPQLNKMIEEYA
                     KTAKITYLDFFSAMADEKNAMKAAYTYDGVHCTSAGYDVMEAMVVPAIQKALKTKPRK
                     K"
     gene            complement(417..1172)
                     /locus_tag="PLN75_08480"
     CDS             complement(417..1172)
                     /locus_tag="PLN75_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRG04191.1"
                     /translation="MEKVLNLTLKVWRQNGSKVKGAFETYKLDGISPDCSFLEMMDIL
                     NEKLISEHKEPVAFDHDCREGICGMCSMYINGHPHGPDGEITTCQLHMRNFKDGATIT
                     IEPWRSAAFPVIKDLMVDRGAFDKIIQAGGYVSVNTGGVPDGNAIPIPKPAADEAMDA
                     ASCIGCGACVAACKNGSAMLFLSAKVSQLALLPQGRVEAAHRAKAMLAKMDELGFGNC
                     TNTGACEAECPKNVSISHIARLNREFISAKFKD"
     gene            complement(1284..3227)
                     /locus_tag="PLN75_08485"
     CDS             complement(1284..3227)
                     /locus_tag="PLN75_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRG04192.1"
                     /translation="MTRKDAKIPEGPLAEKWSNYKAHQKLVNPANKRRLDVIVVGTGL
                     AGASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYPNDGDSVYRLFYDTI
                     KGGDYRAREANVYRLAEVSNNIIDQCVAQGVPFARDYGGLLDNRSFGGAQVSRTFYAK
                     GQTGQQLLLGAYSALSRQVGKGTVKLYVRYEMLDVVVIDGRARGIIARNLVTGKTERF
                     FAHAVVVGTGGYGNAFFLSTNAMTSNGSAAIQMYKKGAYFANPAYAQIHPTCIPVHGD
                     YQSKLTLMSESLRNDGRIWVPKKKEDAEAIRAGKLKPTDLKEEDRDYYLERRYPAFGN
                     LVPRDVASRAAKERCDAGFGVGNTGLAVYLDFSEAINRLGENVVRARYGNLFQMYEKI
                     VDDNPYKTPMMIYPAIHYTMGGVWVDYELQTSVPGLFCIGEANFSDHGANRLGASALM
                     QGLADGYFVLPYTIQNYLADQIQVPRISTDREEFEQAEKAINERIAKIMSIQGKYSVD
                     HIHRELGLVMWDYVGMGRTAEGLKIALEKIKAIRNEFWTNVYVPGKSEGLNVELEKAL
                     RLADFIEIGELMARDALLREESCGGHFREEYQTPEGEALRNDEKFSFVSCWKYTGEGS
                     EPELVKEPLDYEFIVRQQRNYKA"
     gene            complement(3547..>4158)
                     /locus_tag="PLN75_08490"
     CDS             complement(3547..>4158)
                     /locus_tag="PLN75_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HRG04193.1"
                     /translation="TFHSIMNVTVLFSAQVYNDICEILGANWYAILGTGALALGFVLH
                     ILYALYLTLLNLKARGKDRYAVTQRQEGVAWASKNMFVLGLIIFGFLVLHLFNFWAKM
                     QLVELTHGHGWAEAGLPNPQEGAFFINELFSNPVYSIIYIVWLAALWFHLTHGVWSSL
                     QSIGVNNKTWLPRVKCVANVYSTIIIILFMAIPVYFLLGFRVL"
BASE COUNT         1079 a         1005 c          894 g         1180 t
ORIGIN      
        1 ccgaactctc tactgccaat aatattcggg tagtgctttg ctcggtactt ccggcttacg
       61 attatccatg gagaaaaggg ttggaaccca atatcaaaat tcctcaactc aacaaaatga
      121 ttgaagaata tgcaaaaacc gcaaaaatca cttacctgga ttttttcagc gctatggccg
      181 acgaaaaaaa tgccatgaaa gcggcataca cttatgatgg agtgcattgt acatcagccg
      241 gatacgatgt gatggaagct atggtggtgc ctgccattca aaaagcattg aaaaccaagc
      301 cacgaaaaaa gtaacaatca tccaactacc aaaaaagtcc gctgcagcac ctgcaacgga
      361 cttttttatt tctgatcaat ccaaaagcct cctttttggg aaggtttgga gggattttag
      421 tctttgaatt ttgcactgat aaactctctg ttcaatcggg caatgtgcga aatagataca
      481 ttcttcgggc attctgcttc gcaggctccg gtatttgtac agtttccaaa tccgagttca
      541 tccatcttgg caagcatggc tttggcacga tgagcagctt ctaccctgcc ctgtggtaat
      601 aacgccagct gacttacttt agctgagagg aacaacatag cagaaccatt cttacaagca
      661 gctacacaag caccgcatcc gatacaggaa gcagcatcca tcgcctcgtc ggcagcaggt
      721 ttcggaatcg gaattgcatt tccatcaggt acaccgcctg tattgaccga gacataaccg
      781 ccagcctgaa taattttgtc gaaagcacca cgatccacca tcaaatcctt gataacagga
      841 aatgctgccg aacgccacgg ttcaatggta atagttgcgc catctttgaa attgcgcatg
      901 tgcagctggc aggtagtgat ttctccgtcg ggtccgtgcg gatgtccgtt tatgtacatg
      961 ctgcacatac cgcagatacc ttcgcgacaa tcatgatcga atgcaaccgg ttccttgtgt
     1021 tcacttatca gtttttcgtt cagaatatcc atcatttcga ggaatgaaca atcgggtgat
     1081 atcccgtcta atttataggt ttcgaaagca cctttgactt tcgaaccgtt ttgacgccat
     1141 actttcagcg tgagatttaa tactttttcc atataagccc cctttaattc ccccgaaggg
     1201 gaaattttga agttaatttt ataattaatt atcattatag cacgctctaa aaatctcccc
     1261 ttcggggaga tttagagggg ctgttatgct ttataattcc gttgttgtct gacaatgaat
     1321 tcgtaatcca gcggttcttt gaccaattca ggttcactgc cttcgccggt atatttccag
     1381 cacgatacaa atgagaattt ctcgtcgttt cttaatgctt ccccctcggg agtctggtac
     1441 tcttcgcgga aatggccgcc acagctttct tcgcgaagca atgcatcgcg tgccatcagt
     1501 tcacctattt cgataaagtc ggccagacgg agcgcttttt caagttccac attcagacct
     1561 tcggattttc cgggtacata tacatttgtc cagaattcat ttcgtatggc cttaattttt
     1621 tctaaggcga ttttcaggcc ttcagccgta cgtcccatac ctacataatc ccacataacc
     1681 aaaccgagtt cacgatggat atgatctacg gaatacttgc cctgaatact cattattttt
     1741 gcaattcttt catttatagc tttttcagct tgctcaaatt cttcgcggtc ggtactgatg
     1801 cgcggtacct gaatctgatc tgcaaggtaa ttctgaatgg tgtacggcag tacgaaatat
     1861 ccgtcggcca gcccctgcat gagtgctgat gctcccagcc ggttggctcc gtgatcggag
     1921 aagttggcct cacctataca gaacaagccc ggaacagagg tttgcagttc gtaatccacc
     1981 cacactccac ccatggtgta gtgaatagcc ggataaatca tcatcggagt tttatatgga
     2041 ttgtcgtcca caattttttc gtacatctgg aataaattgc cgtaacgggc tctgaccaca
     2101 ttttctccga ggcggtttat ggcttccgaa aaatcaagat aaacggcaag tcctgtattt
     2161 cccacaccga atcctgcgtc gcaacgttct tttgcagcac gtgatgccac gtcgcgaggc
     2221 acgaggttac cgaaagccgg gtagcggcgt tccaggtagt agtcgcggtc ttcttccttg
     2281 aggtcggttg gtttcagttt gcctgcgcgg atggcttcgg catcttcctt cttcttcggc
     2341 acccaaatgc ggccgtcgtt gcggagcgat tcagacatca gggtgagttt ggactggtag
     2401 tcgccgtgaa cgggaataca cgtcgggtga atctgagcgt atgccggatt ggcaaaatat
     2461 gccccttttt tatacatctg tatggctgca gaaccgttgg aagtcatggc attggtcgac
     2521 aggaagaaag cattaccgta tcctccggta cctacaacca ctgcatgagc aaagaatctt
     2581 tctgtttttc cggttaccag gttacgggca ataattccgc gggcacgtcc gtcgattacg
     2641 acaacgtcga gcatttcata gcgaacgtag agttttacgg tgcccttgcc cacctgacgg
     2701 ctcagtgcgg agtatgctcc aagcagcagc tgttgtccgg tctgtccttt ggcgtagaat
     2761 gtacgcgata cctgcgcacc gccgaacgaa cggttgtcga gcaatccgcc gtaatcacgt
     2821 gcaaaaggta caccctgagc cacacattgg tcaataatat tatttgaaac ttcggccaaa
     2881 cgatatacgt tggcttcgcg tgcccggtag tcaccccctt tgattgtgtc ataaaacaat
     2941 cgataaacgc tgtctccatc gttcgggtag ttcttggctg cattaatacc accctgtgct
     3001 gctatggagt gtgcgcggcg tgggctgtcc tgaatacaga agttcagcac attgaaaccc
     3061 atttcggcaa gcgaagcagc agcagaagca cctgctaaac ctgtacccac tacaataaca
     3121 tccagacggc gcttattggc aggattcacc aacttttggt gtgctttgta attgctccat
     3181 ttttcggcca gtggtccttc ggggattttg gcatctttac gggtaatgat tttagtgtcg
     3241 tttttcacat cagcaacaac gcgttgagtc gtagttttga tggtttcaga tacatttgca
     3301 gcactttctt ttgcagtttg tgcaattttt tcgcctgcat ttttcacgga agcaaccacc
     3361 tgtttggttt tgtcgtcttc gatgaaatct tttacatctt caaccagttc ctgtacattt
     3421 tccactaaat cttcggcagt atctttgatg acttcagctg ctttgtttac agcttctttt
     3481 gccttatcga aaatatcttc tttctttgcc ataattctta tattttttta tatttaaaca
     3541 caggcattac aacacccgaa aacctaataa aaagtaaacc ggtatagcca taaacagtat
     3601 aatgataatg gttgaataca cattagcaac acatttcaca cggggtaacc aggttttgtt
     3661 gttcaccccg attgactgaa gcgaactcca tacaccatgc gtaaggtgga accataatgc
     3721 agccagccac acaatgtaaa tgatcgaata aaccggatta ctgaaaagtt cgttgatgaa
     3781 gaatgctcct tcctgtggat ttggcagacc tgcttcagcc cagccatgtc catgagtgag
     3841 ttctaccaac tgcatttttg cccagaaatt gaataagtgc aggacaagaa atccgaaaat
     3901 aatgagaccg agcacaaaca tattttttga tgcccaggct acgccctcct gacgttgagt
     3961 aacggcataa cggtctttac cacgcgcttt gaggttaagc agggttaaat acagcgcata
     4021 taaaatatgc agtacaaaac ccagtgcaag tgctcctgtg ccaagtatgg cataccagtt
     4081 tgctcccaaa atttcgcaaa tatcattata aacctgagca ctgaaaagta cagtgacatt
     4141 cataatgctg tgaaatgt
//
LOCUS       DAORKP010000176         4136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5634_length_4136_cov_5.475619, whole genome shotgun sequence.
ACCESSION   DAORKP010000176 DAORKP010000000
VERSION     DAORKP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5634_length_4136_cov_5.475619"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1377)
                     /locus_tag="PLN75_08495"
     CDS             complement(115..1377)
                     /locus_tag="PLN75_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG04194.1"
                     /translation="MDKRHLQEILIDQKEAFQHKTGLIQRSLPLDNYLHTAQVVVITG
                     VRRCGKSSLMFLIKEAMGLSESEFCYCNFDDERITCYPALLNDIYTLHLEMYRSEPVF
                     FFDEIQEVPGWEKFVNRMYEQGRKVFVTGSNATLLSSEISSSLTGRNKVLELFPFSFP
                     EYLRMEGKTYEPDRLSVKQRSLLLNDLNSYLEIGGFPLVVREKDPEIAHALFQDILYR
                     DIVVRYKLSNVEEVRQIALYLASNTGKLFSYATLQTISGLKSLSSVKNYLQYFEASYL
                     FCYLKKFDYSVKKQIMNSRKVYAVDNAIPNRLGFRFSDNKSRLLENAVFIELQRRGCE
                     IYYFSEKYECDFLVKENLDITEAIQVTYGLNAENVHREIGGLAEAMQTFNIPKGTLIV
                     FDDTNFPVDLPPGVSVIPAYEWLMEVTR"
     gene            complement(1798..2940)
                     /locus_tag="PLN75_08500"
     CDS             complement(1798..2940)
                     /locus_tag="PLN75_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRG04195.1"
                     /translation="MNKKIWLSLAKMGGREREFIREAFETNWVAPLGPNVTGFEKDLE
                     AYFDSDTHIVALSSGTAAIHLALIQLGVEPGDEVICQSFTFVASANPITYLGASPVFV
                     DSEPDTWNMSPEHLRRAIADRIAVTGRKPKAIIPVHLYGMPANMDAILAIAEEYDIPV
                     LEDAAEALGSIYKSQHCGTFGAFACLSFNGNKMITTSGGGALVCKSREAAHKTLFYAT
                     QARDQAPHYQHSEIGYNYRMSNVSAGIGRGQMFVLDDHIAHRRSINALYRELFRNVKG
                     ISVHQEPNDDYNSNHWLTCITLDTPLNGHTPYKISKALEAENIESRPLWKPLHLQPVF
                     EQAPFYGDGTSEHLFNRGLCLPSGPHVSEEDAIRVVEVIREVIRKD"
BASE COUNT         1071 a          907 c          900 g         1258 t
ORIGIN      
        1 caaaggataa aaatcttaga tttttataaa ttatgacttt tcggagtgga ctcaactata
       61 caattcaaca gtcatcacta cctaatcctg aacccgctag tgaagggcag ggggctatct
      121 tgtcacttcc atcagccact catatgccgg tattaccgat actcccggcg gtaaatcaac
      181 cggaaaatta gtatcatcaa atactatcag tgttccttta gggatattga atgtctgcat
      241 cgcctctgct aatccaccta tttcgcgatg gacattttcg gcattcagcc cgtaagtaac
      301 ctgtatagct tccgttatat ctaaattttc ttttacaagg aaatcacatt cgtatttttc
      361 tgaaaaataa tagatttcac aaccccgcct ctgcagctcg atgaatacgg cattttccag
      421 tagccggctt ttattatccg aaaaccgaaa tcccaaccgg ttgggtattg cattatcaac
      481 agcgtaaact ttgcgggagt tcatgatttg ctttttgacc gaataatcga attttttcaa
      541 ataacagaac aggtagcttg cttcaaaata ctgcaaatag tttttcaccg aactgaggct
      601 tttcagaccc gaaatagttt gtaaagtggc gtacgagaat aatttaccgg tattggatgc
      661 caggtaaagc gctatctgcc ttacttcttc tacattgctg agtttataac gcaccactat
      721 atcgcggtag agtatatcct gaaacaatgc atgagcaatt tccggatctt tttccctcac
      781 cacaagcggg aaacccccga tttcaagata agagttcaaa tcattcagca gcagggagcg
      841 ttgttttacc gacaaccggt caggctcata tgtcttacct tccatacgca agtattccgg
      901 aaatgaaaat ggaaataact ccagcacttt gttccgaccc gtaagcgacg aggatatttc
      961 ggagctgagc aaagtggcgt tggagccggt tacaaatact ttgcgacctt gttcgtacat
     1021 gcggttgacg aatttttccc agcccggtac ttcctgtatt tcgtcaaaga aaaaaaccgg
     1081 ttcacttcga tacatttcca aatgaagcgt atatatatcg ttgagcaatg ccggataaca
     1141 ggttattcgc tcatcatcga aattgcagta acaaaattcg ctttcgctca aacccattgc
     1201 ctccttaatc aaaaacatca gcgatgattt cccgcaacgg cgtactccgg ttataactac
     1261 tacctgagcg gtatgtaaat aattgtccag tggcagcgaa cgctgaatta agccggtttt
     1321 gtgttgaaac gcctcttttt gatcgatcaa tatttcctgt aagtgtcttt tgtccatctt
     1381 ttgacttata tttcgcgaca aagataacaa tttattcttt caaaaagaac aatatacgtc
     1441 atttttattc ttttacaacg aacaatttct ctctacatct acctgttaat tgaactttcg
     1501 catgttcata ttagtttata aatcaaaaga ttaagaatga ataatatctg acagtcacat
     1561 agatatacat tttttcagaa acagactaca attttatctg tttttcttcg catgtcgttg
     1621 aaacagcccg tccggaaacc ggtcgtcgag atgcgacctg aagatcaaac ttttcttctt
     1681 cgacgaatga gaaaaaacgt ttttttttga ttcaatttat agaagcatac ggccttctga
     1741 tggcatcaga gaaacgtctt ttcttagggg cttattcttc attcttaaaa ttctttgcta
     1801 atctttcctt atcacttccc ggatcacttc cacaaccctg attgcatctt cttcgctaac
     1861 gtgtggtccg gaggggaggc agagtccgcg gttgaagagg tgctcgctgg taccgtcgcc
     1921 gtagaagggt gcctgctcaa ataccggctg gaggtggagc ggtttccaga gcggacgcga
     1981 ttcgatattt tccgcttcga gggctttgct tattttgtag ggtgtgtgtc cgttgagcgg
     2041 agtgtcgagc gtgatgcagg tgagccagtg gttggagttg tagtcgtcgt tgggttcctg
     2101 atgcacactg atgcctttca cgttgcgaaa caattcgcgg tacagggcat ttatgcttcg
     2161 gcggtgcgct atgtgatcgt cgaggacgaa catctgtccg cggcctatac cggccgaaac
     2221 gttactcatg cggtagttgt atcctatctc ggagtgctgg tagtgaggtg cctggtcacg
     2281 tgcctgcgtg gcatagaaaa gtgttttatg tgccgcttcc cggcttttac atacaagggc
     2341 tcccccaccc gatgtggtga tcatcttgtt gccgttgaac gacaggcagg caaaagctcc
     2401 gaaagtgccg cagtgctgac ttttgtaaat ggagccgagg gcttcggcag catcttcgag
     2461 caccgggatg tcatactcct cagcgatggc caggatggca tccatattgg ctggcatgcc
     2521 gtagaggtgt acggggatga tggctttcgg tttacgacct gtaacggcaa tgcggtcggc
     2581 aatggcacgg cggaggtgtt cgggcgacat gttccacgta tcgggttcgc tgtccacaaa
     2641 taccgggctg gctccgaggt aggtgatcgg attggccgaa gccacgaatg taaaactctg
     2701 acagatcacc tcatcgccgg gttctacacc gagctgaata agcgccaggt gaatggctgc
     2761 cgtaccgctg ctcagcgcca cgatgtgtgt gtccgaatca aaataggctt cgaggtcttt
     2821 ctcgaaaccg gtaacgttag gtcccagcgg agccacccag ttggtttcaa aagcttcgcg
     2881 gataaattct ctttcacgcc cgcccatttt ggccagcgat aaccagattt tcttgttcat
     2941 gtagtttatt taaaaagtat tacagttaaa tttttctcct gccgaacttg ccggaggatg
     3001 tcattgatgt ttgaaaaatt aacagtaagg ttttatattt atttggggta agtttttttg
     3061 tattttcgtt tactcagata aaaaaacacg ttatcgaaca acttgtccgt ctcccgatag
     3121 caatcggaac gaaaattgtt tcgtatggca gcagatttta ctatggggag cagtacaagc
     3181 tgtgaaactt cagttgacgt cagcaaggct tttttcgggt tacaaccaca aagatagcct
     3241 gttttttatt tttttttatg gttataaccc ttaaaatatt ttatcggtag caaactttta
     3301 tcactcgtca gtcagagccc ggatttatgt tcaaaaactc ttcgtcttaa ttcattggtt
     3361 acacccgtat aaaggactat attgtttccc gtagtgagaa tataaacata gtagaggtgt
     3421 aatttcatat ttgtattttt gttccctcac tccgctgcgt tccgttctgc ttgcctgcct
     3481 ctggcagggt taggggcaag tctgtgccgt caaccacaac aacccgaata cagggctgat
     3541 gaggataagt ccaatcagcg aaagggtaaa gtcgagcggg cgtttgagaa agtttttata
     3601 catgttattg catgttgtct aattaatacc cttttctgtc cctcgtttcc taacgaggag
     3661 gagtaccgtg tcgtggtcta tttttcttct ttggcactta aaaaccttat tttatatact
     3721 gaatattcaa accttagtag taaattacga atgataaatc ccaaccttat tatcttattt
     3781 taatcatctt aatgaggtaa taaggttatt ttttttgaat gtttttttct actaaggttt
     3841 taaataaaag taaataaggt tttatcctac cggcagcggt gggaaacttc cggcacccat
     3901 cgtgttaaaa agctacatta tactcataaa tcctatcgac gggcttatga aaaaacgtga
     3961 ccttgtcatt ccgaagttcc ccttttgggc ttgaccgcct caggaggtgg agttcccgat
     4021 aaatcgggac acggtggagg gggctttcat catcctccac agccctttta atcctttcca
     4081 atgccttgtg gggtaataga gccagaaaag cgacggatgt ttcatcgcgt agcttc
//
LOCUS       DAORKP010000177         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_5821_length_4068_cov_8.608522, whole genome shotgun sequence.
ACCESSION   DAORKP010000177 DAORKP010000000
VERSION     DAORKP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5821_length_4068_cov_8.608522"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLN75_08505"
     CDS             <1..957
                     /locus_tag="PLN75_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04196.1"
                     /translation="VPGNPDSVCTCTKDVRENYEQALSNLYSIVTEDSQERKNRVDEY
                     MNQNEDRMKASMMKEMSEKTGISQQELETLSKKKDLTQEDKQALMEKALGQHNLSMGE
                     IQNMKNMDENARKAWAQAYATEQMAMAQVNKDNPKMKASNDKAMNQVALMQEQQNLRN
                     QLSAFETEIVNRYKTLIQNSGKEKLEADLLKLNNELRTIGSIYECNECPAPPAEDVKH
                     WNAVIKKIQDTKIQYCKLNTPKMIAYLSWYKENLIKFIPVYDKSEEIQYKTTSATTNT
                     QLTPLDKGIFSLQALQGYLSAKQRLFEFKTYDKENFASDFIK"
     gene            1122..3335
                     /locus_tag="PLN75_08510"
     CDS             1122..3335
                     /locus_tag="PLN75_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG04197.1"
                     /translation="MKSFFTIVFLFISMALSAQGNDAEAIRRQMAKVRQSTNWSDPAA
                     ARKATAEIQRLAAKLTGNQNLPVIGGSDTPTPAAKQTEIKPAENPNKENVLRIAERFY
                     NRSYKQLNAIEKSRFDLEYQNAQKNKFNNQAVKTLTSNGANLMMFGTNHHEGCVFLAA
                     AVKADINDTLAVNNFGAYLRIIDSLKVSLPVLLYANTLFDKSPVILTQIGCSYFELND
                     FKKGENYLKLALQYNPGFGKAHSALCELYLKTGRWKDALQELIAGVVNQGCSYMQAHS
                     NFQQLADVSSSKSGGGDSGSAGKAQGLYNQNNSSTDDDALAPLVPQNKGKVQIDPKDM
                     LASLTPASEADIPDNEKLAPLVPDENKLRMPQFSNITSVNDWMEGGGYSAAITAFQDF
                     MTTNKAAALELHQVLTEQPNLPPDAVLRDYPNERFAIDCITEMIADMSNDESMALSGK
                     TDNYMKQALESQQVFIDKFQRISKDYVSCMEACPTENPMHDYCAGECRRKYCLEMCNA
                     ANSYNKDLQNIFSDCTRDFNESKNRQEKLLDDLYGFTTPWFKKLYSPYWSKIEAYEIR
                     RVALLIIGNTISGYTYRIFPYPGHTDCGSDCSVFVNAAPPPVEEVEKKNPEGNECPFE
                     GNKLSLGVGLCSVDFDCESIEAGCSAGVSISAKRNFKNKSSTLFVGAGGEANLGAVSG
                     EIKTGLTFTKYDNGNMDVGGKFEMTGKVGPVGKNYEMTATLMEGANIEGKNVIGL"
     gene            3392..3853
                     /locus_tag="PLN75_08515"
     CDS             3392..3853
                     /locus_tag="PLN75_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04198.1"
                     /translation="MKTTNSFFAVFCLILAVLSFSGFRSGRESGMNTSEPGSLTVTTQ
                     NVLDIKSNRASCTFTITGSPVQEKGVCFSQSPNPTISNSKSMAPSNPTNSGKAILSGL
                     KPSTKYYVRAYAKSGSNVFYGNELSFTTTNDEQKSNSNFGQKKESNTGGSK"
BASE COUNT         1320 a          856 c          862 g         1030 t
ORIGIN      
        1 gttcccggaa atcccgattc ggtttgtacc tgcaccaaag atgtacgcga aaattacgaa
       61 caggcattga gtaatcttta tagtattgtt acagaagatt ctcaggagcg aaaaaatcgg
      121 gtagatgagt acatgaacca aaacgaggac agaatgaaag cttccatgat gaaagaaatg
      181 tccgaaaaaa caggtatttc tcagcaggag ctggaaacgc tgtctaaaaa gaaagatctg
      241 acacaggaag acaaacaggc gctaatggaa aaagcactcg gacaacataa tctgtcgatg
      301 ggcgaaatac agaatatgaa aaatatggat gaaaatgccc gtaaagcctg ggcacaggct
      361 tacgctaccg agcaaatggc tatggctcag gtgaataagg ataatcctaa gatgaaagcc
      421 agcaatgaca aagcaatgaa tcaggttgca ctcatgcagg aacagcaaaa tctcagaaat
      481 caactcagcg ctttcgaaac ggaaatagta aaccgctaca aaacacttat acagaattca
      541 gggaaggaga aactggaagc tgatttgcta aaactgaaca acgaacttcg tacaatcgga
      601 agtatctacg aatgcaatga atgtccggct ccacccgccg aagatgtaaa acactggaat
      661 gctgtgataa agaaaattca ggataccaaa attcagtact gcaaactaaa tacgccgaaa
      721 atgattgcct atctgagctg gtacaaagaa aatctgataa aatttattcc tgtttatgac
      781 aaatctgaag aaatccagta taaaacaact tctgctacga ctaatacgca actaactcca
      841 ctcgataaag gcatcttttc acttcaggca ctacagggat atttgtcggc aaaacaaaga
      901 ttatttgaat tcaaaaccta cgacaaagaa aattttgctt cagatttcat caagtgaatt
      961 ttttaatgtt catgccgatt taacggctgt atgttttttt gagattgaaa cgctcttgtc
     1021 cgaacggaca ctgactaacc cggatattgg gtgtttttta acggaatttg ttccgtttat
     1081 tctgagtaat tgacttctaa tcaaataatc tctttattca gatgaaatca ttttttacaa
     1141 ttgtttttct gttcatatca atggccttat cggcacaggg caatgatgcg gaagccattc
     1201 gccgccaaat ggccaaagtg cgccaaagca ccaactggag cgaccctgca gctgcccgga
     1261 aagccactgc cgaaattcag cgactggctg ccaaacttac cggaaaccag aacctacccg
     1321 tgataggcgg ttcggataca cccacaccag ccgcaaaaca aacggagata aaaccggcag
     1381 agaatcctaa caaagaaaat gttctgagaa ttgccgaacg gttttacaac cgaagttaca
     1441 aacaattaaa tgctattgaa aagagccgtt tcgacctcga atatcagaat gctcagaaaa
     1501 acaaattcaa caatcaggcc gtgaaaacgc tgaccagcaa cggtgcaaat ctgatgatgt
     1561 ttggaaccaa ccaccacgag gggtgcgtat ttcttgctgc ggctgtgaaa gccgatatca
     1621 acgacacact ggctgtgaac aattttggag cttacctgcg catcatcgac tcgctgaaag
     1681 tatcgctgcc ggttttgcta tatgccaata ccctgttcga caaaagtccg gtcattctca
     1741 cccaaatcgg atgcagctat tttgaactga atgactttaa aaaaggggaa aactacctca
     1801 agctggcttt gcagtataat cccggcttcg gaaaagccca ttcggcactg tgcgaacttt
     1861 acctcaaaac cggccggtgg aaagatgcat tgcaggaact gatagccgga gtggtcaatc
     1921 agggctgctc ttatatgcag gcacattcca attttcagca gctggctgat gtgtcatcct
     1981 cgaaaagcgg tggcggtgac agtggcagcg ccggaaaggc tcagggatta tacaatcaaa
     2041 ataattcctc caccgacgat gatgcattgg caccactggt gcctcagaat aaaggtaaag
     2101 tgcaaattga cccgaaggac atgctggcat cgctcacacc cgcttccgaa gccgacattc
     2161 cggataatga aaaactggca ccgttagttc ctgatgaaaa taaactaaga atgcctcagt
     2221 tttccaatat aaccagtgtg aatgactgga tggaaggagg cggatattcg gcagccatca
     2281 cggcttttca ggattttatg acaacgaata aggcagctgc tcttgaactg catcaggtac
     2341 ttactgagca acccaatctt ccgccagatg ccgttttgcg tgattacccc aacgaacgct
     2401 ttgccattga ttgcatcaca gaaatgatag ccgatatgtc caacgatgaa agtatggcat
     2461 tgtccggtaa aactgacaat tacatgaagc aagcacttga aagtcaacag gtatttatcg
     2521 ataaatttca gcgcatttcc aaagattacg tgagttgtat ggaggcttgt ccgaccgaaa
     2581 atccgatgca tgattactgc gccggcgaat gtagacgtaa gtattgtttg gaaatgtgca
     2641 acgctgccaa ttcatacaac aaagatctgc agaatatttt cagcgactgt acccgggatt
     2701 tcaacgaaag taaaaaccga caggagaagc tgctggatga cctctatgga tttacaaccc
     2761 cgtggtttaa gaaactttac agtccgtact ggagcaaaat cgaagcgtac gaaattcgcc
     2821 gtgtagcttt gttgatcatc ggcaatacca tttccggata tacttatcga atatttcctt
     2881 atccgggaca taccgactgc ggctccgatt gttccgtttt tgtgaatgct gccccgccac
     2941 cggttgaaga agttgagaag aaaaatcctg agggaaatga atgtccgttt gaaggcaata
     3001 aactatcgct tggtgtcggt ttatgcagcg tcgatttcga ttgcgaaagc attgaagccg
     3061 gctgctcggc aggagtttcc atttcggcca aacgaaattt caaaaataaa agctccaccc
     3121 tgtttgtcgg cgccggtggc gaagccaatc tgggagctgt ttcgggtgaa atcaaaaccg
     3181 gtttaacctt taccaaatac gataacggta atatggatgt aggaggaaaa tttgaaatga
     3241 ccggaaaagt aggtccagtg ggtaaaaact acgaaatgac ggctacactc atggaaggtg
     3301 ctaatattga aggtaaaaat gtaatcggat tataaaatat ctcactgagt aaactcttat
     3361 tttcaacttt ttttcataaa tactaataac catgaaaaca acaaattctt tttttgctgt
     3421 attctgcctg atactggcag ttttatcgtt ttcgggcttc agatcggggc gtgaaagtgg
     3481 aatgaacact tccgaaccgg gcagtctgac tgtaactacg cagaatgttc tcgacatcaa
     3541 atctaacaga gcatcatgta cttttaccat cacgggctca cccgttcagg agaaaggagt
     3601 ctgcttctca cagtcaccca atcctaccat tagtaattca aaatcaatgg ctccctccaa
     3661 tcccaccaat tcaggaaaag caattctttc cggactaaaa ccatcgacca aatattacgt
     3721 cagagcctat gctaaaagtg gttcgaatgt tttttatggc aacgagttaa gttttactac
     3781 gactaatgat gaacaaaaaa gcaactccaa cttcgggcaa aagaaagaat ccaataccgg
     3841 tggaagtaaa tagagtggtt atcaagactt aatatttgtt taaaacgatt atcggcaata
     3901 attttttaaa aatccccctt tttgggggtg ttttttaaaa taaaattaaa ataattttgc
     3961 agagttaaca atttataaca tagattccta agcaaatact tttttatttt tatattttcg
     4021 tgcattgatc aggaattgat aacttttggg caatgatgga aaatcaat
//
LOCUS       DAORKP010000178         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6027_length_3984_cov_8.681089, whole genome shotgun sequence.
ACCESSION   DAORKP010000178 DAORKP010000000
VERSION     DAORKP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6027_length_3984_cov_8.681089"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..628)
                     /locus_tag="PLN75_08520"
     CDS             complement(152..628)
                     /locus_tag="PLN75_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRG04199.1"
                     /translation="MDIIIVIALLVLAIAFLLIEFFLIPGFSIAGGTGIVLLAVAVWY
                     AYSHIGPAAGNITLFGSVALTGFAVWLFVKSRALEKMSLKTNITGTVDKIDEEVIKTG
                     DVGVTISRLAPMGKVKVNGVVVEAKTNDEFIDPNEPVEVQEVFKTNVLVKKSEVRN"
     gene            complement(683..2080)
                     /locus_tag="PLN75_08525"
     CDS             complement(683..2080)
                     /locus_tag="PLN75_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRG04200.1"
                     /translation="MKTTERLKITLFAVLILFSAASVAQTPADANTYFESGQYQAAME
                     AYQNLLKKKPKEALYNFRYAYSAYMLKNVSAAVEFFKKSADKFPEASYYLGEMFFDNY
                     AFEEAAAAYSAYLIKPVLNDTLTPKVEKKLKQAELGARLLKRVEDVKIIDSMVVDKND
                     FLKKIELPAEAGTLVQRQLLTGNGKVDNINYTTQRRDRSIFSELNNGQTDLFTADKLL
                     DKWAEKMPMNDLNTDANENYPFLMLDGATLYFAADGEASLGGYDIFITRLNTSDNSFL
                     KPENIGMPFNSPYNDYMMIVDELHRVGWFASDRFQPEGKVAVYQFITNSEKKIVRSVN
                     QDSLISAAQIRKLSKTTEKFNQATENVVTQKETSAGKIFINDTTFYHDASQFKSNIAR
                     NHYFQLQKMIEDMKATQQELENLRSAFIDTKDENQKNETGKKIRSLESRIRTLKPEIK
                     KLSIEMRNEEIKSFP"
     gene            complement(2104..>3984)
                     /locus_tag="PLN75_08530"
     CDS             complement(2104..>3984)
                     /locus_tag="PLN75_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRG04201.1"
                     /translation="VDFKSLLSLIPAIYTNSFAGVKADGKVTVSGFAKGKYEGESYPA
                     FDLQLGVENGKFQYPDLPKSVDNIQIAGHITNPGGSLDNTTVDLSKLNFRMGGNPFTS
                     QLRIVNPVSDPDFNVKAQGKMNLALIKDVYPLEKGTELNGLLDMDVNAAGRMSYVEQN
                     RYESFRFGGTLNVKDVLLKMKDLGQDVAVKNANLLFNDRYLNLTGISLKIGRNDLSAN
                     GKVENYLAYVLRDKTLKGDFNVNSTYLNITDFMGNEEEENDTSSMQIIKIPKNLDLAL
                     AGNFRELVYNKMNLKEANAVMRIADGELNIEKMNVNAFGGNMSLMGRYSSANPEQPAL
                     DFDLDLKQITFADIFAQVPSMQRFVPVFEKLAGRFNTKLSLSTLLQNNMMPVLVSVLS
                     QGNFNAESVTLKEDVTALNELINSLKIDKIKGVNLKEIALAFNIRDGRLETKPFDLRF
                     RDYNLNLGGSTGLDQTIAYAGNVKLPDKLNLGRFQNVGFKIGGTFQKPKIELDLKNTL
                     NTLVEEQKQKALQKADSVKTQVLDKGRAEREKALQEAQKKADVLLEQAKRQGDRLINE
                     AKVRGDSLVAKASNPIARELAKKGAAELVKQAQKQADNLNNKAKAEADKMIQQVTEKT
                     DF"
BASE COUNT          904 a          895 c          837 g         1348 t
ORIGIN      
        1 gggagtttgg atgtttggat gtttggatgt ttggatgttt ggatgtttgg gtgtttgggt
       61 gtttgaatgt ttggatgttt acttactcct tactctgtac tccttactct ctgctctgta
      121 ctccttactc tgtactcctt actccttact cctaatttct gacttctgac ttcttcacca
      181 gcacattggt tttgaatact tcctgcactt ccaccggttc gttcggatcg ataaattcgt
      241 cgttggtttt tgcttccacc actactccgt tcactttaac ttttcccatc ggtgcaagac
      301 gcgaaatggt tactcccacg tcgcctgttt taattacttc ttcatctatc ttatctacag
      361 ttcctgtaat gtttgttttc agcgacattt tttccagcgc acgtgattta acaaacagcc
      421 agaccgcaaa tcccgttagc gctacgcttc caaaaagcgt aatattgccg gcagcgggtc
      481 cgatgtggct gtaagcatac cataccgcca ctgctaagag cacaattccg gtacctccgg
      541 caatggagaa gcccggaatc aggaaaaact caatcagcag aaaagcaata gccaacacca
      601 aaagtgctat aacaatgatt atatccattt ttctattttt atttgggctt aaagttacta
      661 tttatttgct acattacaag gtttagggga aagattttat ttcttcattc cgcatttcga
      721 tgctcaactt ttttatttca ggtttgagcg ttctgatacg tgattcgagt gaacgtattt
      781 ttttccctgt ctcgttcttt tggttttcat cttttgtatc gataaatgcg ctccggagat
      841 tttccagttc ctgttgcgtt gccttcatat cttcaatcat tttttgaagc tgaaaatagt
      901 gatttcgggc tatgttgctt ttgaactgcg aagcatcgtg gtagaaagta gtatcgttaa
      961 tgaatatttt tccggcagaa gtttcttttt gtgtcacaac attttctgtt gcctgattga
     1021 atttttctgt cgttttggat aattttctga tttgcgccgc acttatgagt gaatcctgat
     1081 ttactgaccg gactattttt ttctcagaat tggtaataaa ttgatataca gctactttgc
     1141 cttccggctg aaaacggtca gaggcaaacc atcccaccct gtgaagctcg tctactatca
     1201 tcatgtagtc gttgtagggc gaattgaagg gcatcccgat gttttcgggt ttcaggaaag
     1261 aattatcgga agtattcagt cgtgtaataa agatgtcata tcctcccaga gaggcttcac
     1321 catcggctgc aaagtacagg gttgctccat cgagcatcag aaatggatag ttctcgtttg
     1381 catcggtatt caggtcattc atgggcattt tttctgccca tttatcgagt agtttgtcgg
     1441 ctgtaaagag gtcggtctgt ccgttgttga gctccgaaaa aatggatcgg tcacggcgct
     1501 gtgtggtgta gttgatgttg tccactttac cgtttccggt gagcagctgt cgctgcacaa
     1561 gggtgccggc ttcggcgggt agctctattt ttttcagaaa atcgttctta tccaccacca
     1621 tgctgtcaat aattttgaca tcttccaccc gtttcagcag ccgggctccg agttcggctt
     1681 gtttcagttt tttttctact ttgggagtga gtgtatcatt cagcaccggt ttgatcaggt
     1741 aggccgaata ggctgccgca gcctcttcga aagcataatt atcaaaaaac atttcaccca
     1801 ggtaatagct ggcttcagga aatttatcag ccgatttttt gaaaaattca actgctgcag
     1861 aaacattttt aagcatgtaa gcactgtatg catacctgaa attgtaaagc gcttctttcg
     1921 gctttttttt cagtaaattc tggtaagctt ccatagccgc ctgatattga cccgattcaa
     1981 agtacgtatt tgcatctgcc ggtgtttgtg ccaccgatgc tgcgctgaat aaaatcagca
     2041 cagcgaaaag tgttatttta agtcgttctg ttgttttcat caatatatta tttctgaatt
     2101 tctctaaaaa tctgtttttt cggttacctg ctgtatcatc ttatcggctt ccgctttggc
     2161 tttattattc aggttatctg cctgtttctg agcctgtttt accagttcag ccgctccttt
     2221 tttggccagt tcgcgcgcta tggggttgga tgctttggct accagactat ctccgcggac
     2281 tttagcttcg ttgatgagtc tgtctccctg ccgtttggct tgttcgagca agacatcggc
     2341 ttttttctga gcttcctgca gcgctttttc gcgttcggca cgtcccttat caagcacctg
     2401 agttttcacc gaatccgctt tctgcagtgc tttttgtttt tgctcttcca ccagggtatt
     2461 caacgtattt ttcagatcca gttcaatttt tggtttctga aaagttccgc caattttaaa
     2521 accaacgttt tgaaatctgc caaggttcag tttgtcgggt aattttacgt ttccggcata
     2581 ggcaatggtt tgatccaggc cggtgcttcc gcccagattc aggttataat ccctgaacct
     2641 gaggtcgaat ggtttggttt ccagccggcc atccctgata ttgaatgcca gggcgatttc
     2701 tttgagattt actcccttta ttttatcaat tttaagcgag tttatcagtt cgttgagggc
     2761 agttacatct tctttgagcg tcacagattc ggcattaaag ttcccctggc tcaatacgga
     2821 taccaataca ggcatcatat tgttctgaag gagtgtactg agtgaaagtt ttgtattaaa
     2881 tctgcctgca agtttctcaa atacaggcac aaatctttgc atcgaaggta cctgggcgaa
     2941 aatatcggca aatgtgattt gcttcaggtc aaggtcgaaa tccagtgcag gttgttcggg
     3001 atttgcagag ctgtaccttc ccattagcga catgtttcct ccaaaagcat tgacattcat
     3061 tttttcgatg ttcagttcac cgtcagctat tctcatcacg gcgttagcct ctttcaggtt
     3121 cattttatta taaaccaatt ccctgaaatt tccggcaaga gccaaatcca ggtttttcgg
     3181 tattttgatg atctgcatgg acgatgtgtc gttttcttcc tcttcgttgc ccataaaatc
     3241 ggtaatattc aggtacgtgg aatttacatt gaaatcgcct ttcagcgttt tgtcccggag
     3301 cacgtaagcc aaataatttt ccacttttcc gttggcactc aggtcgtttc ggccaatttt
     3361 cagactgatg ccggtcaggt tcaggtagcg gtcattgaat agcagattag cattctttac
     3421 agccacatcc tgtcccaaat ccttcatttt cagcagtaca tctttcacat tcagagttcc
     3481 gccgaagcga aaactctcgt acctgttttg ctccacgtac gacattctgc cggcagcatt
     3541 cacatccata tccagcagtc cgttcagctc ggtacctttt tccagaggat aaacatcttt
     3601 gatcaatgca agattcattt ttccctgtgc tttcacgtta aaatcggggt ctgaaacagg
     3661 attgacgatg cgaagctgcg aagtgaacgg attgcctccc atccggaagt taagtttcga
     3721 aagatccacc gtggtattgt caagcgatcc tccggggttg gttatatgtc cggctatctg
     3781 aatattatcc accgatttgg gcaagtcggg atattgaaat ttcccatttt cgacacccaa
     3841 ctgcaggtcg aatgcgggat aggattcgcc ctcgtatttt cctttggcaa agccggatac
     3901 agtcactttt ccatctgcct taacacccgc aaatgagttg gtataaatgg ccggaataag
     3961 cgaaagcagc gatttgaaat cgac
//
LOCUS       DAORKP010000179         3980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6037_length_3980_cov_11.325096, whole genome shotgun sequence.
ACCESSION   DAORKP010000179 DAORKP010000000
VERSION     DAORKP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_3980_cov_11.325096"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLN75_08535"
     CDS             complement(<1..197)
                     /locus_tag="PLN75_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HRG04202.1"
                     /translation="MRLRYLLILSISITALSFVGCKPDEPKPDDKPNPFPTFTIPTYA
                     DDYSSISSWANRNKWNLANVH"
     gene            complement(285..2039)
                     /locus_tag="PLN75_08540"
     CDS             complement(285..2039)
                     /locus_tag="PLN75_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG04203.1"
                     /translation="MKSLNIILTTLVLLGGMIACTDQLNVQNPNNQTTYDFGNSESEL
                     QEAVIACYNRIRLEGTFARVGYTLDAVRGDEVWNSSQQWYLEYDNLNSPGTIGIGDEW
                     PWRDNYHVVNRTNFVLSKVDGVKMSVDSYNQIAGQALFLRSLAYYHLATYYQTVPLIT
                     DYASYSDINTMYASNATQDEVFDQIETDLKKAMEMLPSRDKGGEWAKGRATSGAAAGY
                     LARALMFRHKYAEAYAVLKDIIAGKYGHYELTADYGDNFREGPAFENNKESLFEVQFL
                     DFGTGGTDEEWTPVNISSEATQGHAVESNYASQELGSWGDLAGSPWLYNLFKKENSTD
                     GRLDPRLYWTLVTYENEYNTYKGLKTAAYPNGDPRSNTIYQKDITKTPLSNNAQGGIS
                     IAKFTNARNNIYSSITNGLHCGVNLRLMRYSDVLLRAAECENEISGPTQTAIDYINTV
                     RRRVALKDLKLSDFPSADALFEQIANVERPKEFGCENGRGIDLLRWGFFYDAGRLNQL
                     VQHGYYKRDGKASTEELTAETADDSSFKYYYKGHEYFPIFQSTLNANPNLVGNSANKN
                     EDNGPAFKAKWNVRPVVK"
     gene            complement(2070..>3980)
                     /locus_tag="PLN75_08545"
     CDS             complement(2070..>3980)
                     /locus_tag="PLN75_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRG04204.1"
                     /translation="KISAASEYSYDSYGFGTYYDDVHGDLRATNLLLTNSLLKRNSVV
                     DRVVASGSATVDLIGMVGQKTSNHKLDYNLNLSYSKTLVKDFTFVPAFIQSTTNYLSK
                     SNENLSEGYRSYSDALIENTLTYDGKFGLNHINTVVGQTFQRELFHTLTGKGVNLPEP
                     YYLQVNNAEETSSASNESEHVLASYIGRLNYDFDQKYLISATVRRDGSSRLSSADRWD
                     WFPSISAGWRIDRENFFPVAKTNINLLKIRGSYGVLGNENIGEYQYMDVMSRGNYTYS
                     FGNNKVTGSAISNYVNTAIRWEKKKTVDLGVDLGMFNNQLEFTFDWYKSTSEDLLYSV
                     AVPTNAGATNGTVTMNAATMENSGLEFLLAYHNHKHPVKFDISTNLSTINNKVTKLGV
                     SGEPRSDGYCRTEVGREVGSFYGYVYDGIFQSQAEIDNRLNSEGEHINQSGAQPGDVA
                     YADLNLDGEITNEDQKYLGSGLPKFNYGLNARAEWKGLDLSISTYGVAGFKAVDFVDV
                     TLHNSYGALNKSVDLLNAWTPENTNTNVPRVAYKSTGSITNDMFSERFIQDASYLKIA
                     NIELGYNLPDRWFGGYVSGLRIYASAQNLATLSKYKGYNVDFAGGTFTPGYNYCSYPT
                     PRTMMFGIHCSF"
BASE COUNT         1029 a          867 c          907 g         1177 t
ORIGIN      
        1 tcatggacgt tggccagatt ccatttgttt cggtttgccc acgatgatat tgatgaatag
       61 tcgtcagcat aagttggaat agtgaaagtt ggaaaggggt ttggcttatc atcaggttta
      121 ggctcatctg gtttgcaccc aacaaacgat agtgctgtta tgctgataga taaaatcaat
      181 aagtacctta gtctcatgtc cgtaatttta atgttaagaa acttccttat caataaataa
      241 caaatatacc gataaggaag tttccgtttt ttattcaata attcctattt tactacagga
      301 cgaacgttcc atttggcttt gaaagcaggt ccattatctt cattcttgtt tgccgagtta
      361 ccaaccagat tcggattggc attcagcgtc gattgaaata ttgggaaata ttcatgtcct
      421 ttatagtagt atttgaacga tgaatcgtcg gcggtttcgg cagtaagttc ttctgttgaa
      481 gctttgccgt cgcgtttgta ataaccatgt tgaaccaact gattcaaacg gccagcatcg
      541 taaaagaaac cccagcggag caggtcgata ccgcgtccgt tttcacaacc gaattctttc
      601 ggacgttcaa catttgctat ttgttcgaaa agagcatcgg cagaagggaa gtcggaaagt
      661 ttaagatcct taagagctac acgacgacgt acggtgttaa tgtagtcgat agcggtttgg
      721 gttggaccac ttatttcatt ttcgcattct gctgcccgca gcaaaacatc actgtaacgc
      781 atcagacgca ggttgacacc acaatgcaat ccgttagtaa tggaactata gatattattt
      841 cttgcattgg tgaatttagc tatggatatt ccaccctgag cattgttcga tagcggagtt
      901 ttagtaatgt ctttttgata aatcgtgtta ctgcgcgggt caccattggg atatgctgcc
      961 gttttcagcc ctttataggt gttatattca ttttcgtagg taaccaatgt ccagtaaagg
     1021 cgcggatcga gacgaccatc ggtactgttc tcttttttga acaggttata caaccagggt
     1081 gaaccggcca ggtcacccca gctacccaat tcctgtgaag cgtagttact ttctacagcg
     1141 tgaccctgag ttgcttccga gcttatgttt accggagtcc actcttcgtc ggtgcctcct
     1201 gtgccaaagt ccagaaattg cacttcgaag agactctcct tattattctc aaacgcagga
     1261 ccctctctga aattgtcgcc ataatcggct gtaagttcat aatgtccgta tttgccggcg
     1321 atgatatctt taagtactgc ataagcttct gcatatttat gacggaacat cagtgcacgt
     1381 gccagatatc cggctgcagc accgctggta gcgcggcctt tagcccattc gccgccttta
     1441 tcgcgggatg gcaacatttc cattgctttt ttaaggtcag tctctatctg gtcaaacacc
     1501 tcatcctgag tagcattgga tgcatacatg gtgttgatgt ccgaataaga cgcatagtca
     1561 gtaatcaatg gtactgtctg gtaataggta gccagatgat agtacgccag agacctcagg
     1621 aaaagggcct gtccggcaat ctgattgtac gaatctactg acatttttac accgtcaact
     1681 tttgataaaa caaagtttgt acggtttacc acatggtagt tgtcgcgcca tggccattca
     1741 tcaccgatac cgattgtccc cggagaattg aggttgtcgt actccagata ccactgttga
     1801 gaagagttcc agacttcgtc gccacgaaca gcatcgagtg tgtagccaac gcgggcaaat
     1861 gtaccttcga gacgtatacg attgtagcac gcgatgacag cctcctggag ctctgactct
     1921 gaattgccga aatcgtaggt tgtctgattg ttcgggttct gaacattgag ttggtcagtg
     1981 caggcaatca tgcctcccag aagcaccaac gttgttagaa ttatatttaa acttttcatg
     2041 ttgatattct atttattaga ttatattttt tagaatgaac aatgtattcc gaacatcatg
     2101 gttcgtggag tgggatacga acaatagtta tagccgggag taaatgtgcc tccggcaaaa
     2161 tctacgttgt aacctttgta tttggacaat gtggccagat tttgtgccga agcataaata
     2221 cgaagaccgg aaacatagcc accaaaccat ctgtcgggta agttatatcc caattcgatg
     2281 ttggcaattt tcagatagga tgcatcctga atgaaacgct cactgaacat gtcattggtg
     2341 attgaacctg tggatttgta ggctacacgg ggcacgttgg tatttgtgtt ttccggtgtc
     2401 caggcgttga gcaaatcaac acttttattg agtgcgccat acgaattgtg caaagtaacg
     2461 tcaacaaaat cgactgcttt aaaacctgca actccataag tcgaaatgct caggtccaac
     2521 cctttccatt cagcacgtgc attcaagcca tagttaaact taggcaaccc gctgcccagg
     2581 tatttttggt cttcgttggt aatttcaccg tcgagattga gatcggcata agcaacatca
     2641 cccggttgcg caccgctctg attgatgtgt tcgccttctg agttgagccg gttatctatc
     2701 tcggcctgcg actggaatat accgtcgtaa acatagccat agaatgaacc aacttcacgt
     2761 ccaacctctg tgcggcagta accatcggat cggggttcac ctgaaacacc caacttggtt
     2821 accttgttgt tgatggtaga taagttggtg gaaatgtcga acttaaccgg gtgtttatgg
     2881 ttgtgatatg caagcaggaa ctcaagacct gagttttcca tggtggctgc gttcattgtc
     2941 actgttccgt tggtagcacc ggcatttgtt ggcactgcca cactgtacag aaggtcttcg
     3001 gatgttgact tataccagtc gaaagtaaat tccagctgat tgttgaacat accgaggtcg
     3061 acacctaagt ccactgtttt cttcttttcc caccggatgg ctgtgtttac gtagttggat
     3121 attgcagatc cggtaacctt gttatttcca aaactataag tgtagttacc tctcgacata
     3181 acatccatgt actggtattc accaatattc tcattaccga gtactccata gctgccgcga
     3241 atttttagca gattgatatt cgttttggct acagggaaga aattctcacg gtcgatacgc
     3301 cagcctgccg atatggatgg gaaccaatcc caacggtcag cagaagaaag acggcttgaa
     3361 ccatcacgac gtacggtggc agaaatgagg tatttctgat caaaatcgta gttgagacga
     3421 ccgatatacg atgccagcac gtgttcgctt tcattgcttg ctgatgatgt ttcctctgca
     3481 ttatttacct gcagatagta cggttcgggc aggttgacac ccttgccggt gagtgtgtga
     3541 aagagttcgc gctggaatgt ttgacccact acggtattga tgtgattaag cccaaattta
     3601 ccatcgtagg tcagtgtatt ttcaataagt gcatcgctat agctgcgata gccctctgac
     3661 aggttctcgt tgcttttgga caagtaattt gttgtacttt gaataaaagc aggcacaaat
     3721 gtgaagtcct taacaagggt cttgctgtac gacaggttga ggttgtaatc cagcttgtga
     3781 ttgctggttt tttgaccgac cataccgatg aggtcaacag ttgccgaacc tgatgcaaca
     3841 acgcggtcaa ccacagagtt tcttttcaaa aggctattgg tgagcaaaag gttcgttgca
     3901 cgtaagtcgc catgaacatc gtcgtagtaa gttccaaaac catacgagtc gtagctgtat
     3961 tctgatgctg ccgaaatttt
//
LOCUS       DAORKP010000180         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6165_length_3941_cov_8.850746, whole genome shotgun sequence.
ACCESSION   DAORKP010000180 DAORKP010000000
VERSION     DAORKP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6165_length_3941_cov_8.850746"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1935
                     /locus_tag="PLN75_08550"
                     /pseudo
     CDS             253..1935
                     /locus_tag="PLN75_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
     gene            2440..>3941
                     /locus_tag="PLN75_08555"
     CDS             2440..>3941
                     /locus_tag="PLN75_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRG04205.1"
                     /translation="MAEVRLLNKNYREEPYSAYLFTYFTGNRVHEEAIRFGISRDGYN
                     FFALNDNNPVIDSKQISSTGGVRDPHILRSQDGKTFYMVVTDMTSSKGWDSNRAMVLL
                     KSTDLVNWTSSVVNIQKKYKNQENLKRVWAPQTIFDPEAGKYLIYWSMQHGDGPDVIY
                     YAYANDEFTDITGEPKPFFFPENKKSCIDGDIVLKDGIYHMFYKTEGHGNGIKLATTN
                     SLTSGKWTEYPDYKQQTKDAVEGAGVFKRINSDKYILMYDVYTKGKYQFTESYDLQNF
                     NVIDQSVSMDFHPRHGTIIPITQQELEKLTAKWGTPGKFPESGHNPVIKGFYADPSVV
                     YSNKTNKYYIYPTSDGYDGWSGTYFKTFSSPDLYNWTDEGVILDLKKDLTWADRNAWA
                     PAITEKKINGKYKYFYYFTAAQKVGVAVSDDPGGPFKDSGQPLINFKPEGINRGQEID
                     PEVFTDPKTNKSYLYWGNGYMAVAELNADMTSIKKNTIKVITPDKTFREGT"
BASE COUNT         1232 a          857 c          878 g          974 t
ORIGIN      
        1 caaaaataca cttgtagttc acttcctcta atttcaacaa gaagataacc gtagctgaaa
       61 cgatgaattt atatttataa ctttcttaca gagtctatta tgagtaattt taaatcagga
      121 tttgtttttt tcactctttt aatttttggt acattagctg tttttgcaca gacggatcag
      181 aaaatttcgt acttcagtcc cgaggatgtt cgtcttaccg agagtccgtt caggcatgcc
      241 gaagaaatgg atatgaagta cctgctcgaa ctcagtgccg atcgtctgtt ggctccttat
      301 ctgcgtgaag ccggactcac accgaaagct gaaaattatc caaattggga aaataccggc
      361 ctcgacgggc atataggagg acattacctg tcggcacttg catacatgta tgcatctaca
      421 ggaaatgccg aaataaaaac ccggctggac tacatgatca gcgaactgaa aaaagcgcag
      481 gatgccaacg acgacggata catcggggga gtgaccggag gcagggccat gtggaaagaa
      541 attggcgatg gcaaaattga ggctgccagt tttggactga atggtaaatg ggtacctctt
      601 tacaatatcc ataaaacttt cgcgggtttg cgtgatgctt acctgacagc cggcagcgaa
      661 caagcacgtg agatgctgat taaatttggt gactgggctg taaatctatt ctcgaaactt
      721 accgacgagc aaatccaaaa catgctcagg agcgaacagg gaggcctgaa cgaggttttt
      781 gcagacatca cagccatcac aggcgatgat aaatacctga aactcgcccg cagattctca
      841 caccggtata ttctcgagcc tcttttgaat aaagaggaca aactcaccgg acttcatgcc
      901 aatacacaga ttcccaaggt gataggattc aaaagaattg ctgaactgga tggaaacaaa
      961 gcctggagta atgcagccct ctttttctgg gaaaatgtaa ccgaaaagcg ttcggtaagt
     1021 atcggcggta acagtgtttc ggagcatttt cacccggtgg atgattattc acgcatgctg
     1081 agcagcatcg aaggaccgga aacatgtaac acctacaaca tgctacgcct tacaaaattg
     1141 ctctataaga cttatcccga aacaagattc attgatttct acgaaaaagc gctttacaat
     1201 cacatactat cctcacagaa tccgaatacc ggaggacttg tgtatttcac tcccatgcgc
     1261 ccgggacatt acagagtata ttctcagact cagaccagct tctggtgctg tgtgggctca
     1321 ggtatcgaaa atcatgctaa gtatggcgaa atgatatatg cacattcggg taataacctg
     1381 tatgtcaatc tttttattcc atcccgatta aactggaaag agaaaagtac tgaaatcgtg
     1441 caggaaaata acttcccggc cgatgcgcag actcagttga ccattcatcc gaaaaaaaca
     1501 acccgcttta cactgaaaat ccgctatcca cagtgggttg caagtcctca gcccgaagtc
     1561 actatcaacg gaaaacctgt ttcagtggct catgttgatg gatatatcct gattgacagg
     1621 aaatggaaaa aaaacgataa agtcaacgtg aaattgccca tgaaggttgt tgcagagcag
     1681 attcccgaca aatccagcta cttttcattc cgctacgggc ccatcgtact ggcggcaaaa
     1741 accggcactg aaaacatgga tggaacgttt gccgatgata gtcggggcgg gcatattgca
     1801 cacggcaaac agatttcgca aaaagaaatg ccgataatag tcggtgaaaa agacaatctt
     1861 gcctcgctgg taaagcccgt ggccggcgag ccgctcacgt ttaaacttac aggtctgttt
     1921 cccgaaaagt attcggatgg aatgacgctg attcctttcc acaaattgca cgaatcccgc
     1981 tacatcattt actttgctca ggcctctgtc gaagaagtgg attcaatccg gcggaagatt
     2041 gaaaaggaag aacaggaaaa aatcaggctg aatgccatga cagttgatgg ggttgtatgc
     2101 ggcgagcagc aaccggaatc tgatcatttc atccaatcgg aaaattcgca gacaggtttc
     2161 gtggaagata ttcagtggcg aatagccaaa ggttggttca gttaccaact ccggaataag
     2221 gaaaaacaag ccagatcgtt gtatgtgaaa ttttttgatg ccgaccgttc gcgaaatttt
     2281 gatattttta tcaatgatgt aagaataacg tctctttcgc tcaccggtaa aaacggaata
     2341 aatctgcaaa cggccgtttt ccccattccc gaaagcgaaa tggataaaga tattctcacc
     2401 gtgaagtttg taccactccc cggaagtgct actgccagaa tggccgaagt acgattgttg
     2461 aataaaaact atcgggagga accgtattcg gcttatcttt ttacctattt cacaggcaac
     2521 agggtgcatg aagaagccat tcggtttggc atcagccgcg atggatataa tttctttgcg
     2581 ctgaacgaca acaacccggt cattgattca aaacagatca gctccaccgg cggagtacgc
     2641 gatccgcata ttctacggag tcaggacgga aaaacttttt acatggtagt taccgacatg
     2701 acttcaagca aaggatggga ttcaaatcgt gctatggtgc tcctgaaatc cactgatctg
     2761 gtcaactgga cttcgtcggt tgtcaacatt cagaaaaagt acaaaaatca ggaaaatctg
     2821 aaacgcgttt gggctcctca gaccattttc gatccggagg caggtaagta tttaatttac
     2881 tggtcgatgc agcacggcga cggacctgat gtcatttact atgcctacgc caacgatgaa
     2941 tttacagata ttaccggtga accgaagccg tttttctttc ccgaaaacaa aaaatcgtgc
     3001 atcgacgggg atattgtttt gaaagatgga atatatcaca tgttttataa aaccgaagga
     3061 catggcaacg gtatcaaact ggccacaaca aattctctga cctccggaaa atggacggaa
     3121 tatcccgatt ataaacagca aacaaaagat gcagtggaag gtgcgggagt tttcaagcgg
     3181 attaattcgg ataaatacat actgatgtac gatgtgtaca cgaaaggaaa atatcagttt
     3241 acagaaagtt atgatttaca gaactttaac gttatcgatc agtctgtttc gatggatttt
     3301 cacccccgac acggtaccat catccccatt actcagcagg aactggaaaa actgactgct
     3361 aaatggggaa ctcccggaaa attcccggaa tcaggtcaca atcccgtaat taaaggattc
     3421 tacgcagatc cgtcggttgt ttactccaat aaaacgaata aatactacat ttacccgaca
     3481 agtgatggat acgatggctg gtccggtact tatttcaaaa cattttcatc gccggattta
     3541 tacaactgga ccgacgaagg agtaatcctc gatttgaaaa aagacctgac ctgggccgac
     3601 agaaatgcct gggcgccggc cattacagaa aagaaaatca acggcaaata caaatatttt
     3661 tattatttca ctgctgctca aaaagtgggt gtggcagttt cggacgatcc tggcggaccc
     3721 ttcaaagatt cagggcaacc acttattaac ttcaagcctg aaggaataaa cagaggtcag
     3781 gaaattgacc cggaagtatt taccgacccg aaaacaaata aaagttacct atactggggc
     3841 aatggctata tggctgtagc tgagttaaat gcggatatga cttccatcaa aaaaaatacg
     3901 attaaagtca ttacacccga taaaaccttc agagagggaa c
//
LOCUS       DAORKP010000181         3921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6239_length_3921_cov_9.050698, whole genome shotgun sequence.
ACCESSION   DAORKP010000181 DAORKP010000000
VERSION     DAORKP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_3921_cov_9.050698"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLN75_08560"
     CDS             complement(<1..215)
                     /locus_tag="PLN75_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRG04206.1"
                     /translation="MEQIIRHISDQIQSEITAFRPVSVGDISSAYLLETQSQKFLLKV
                     NSKSSAKTMFDAEQQGLRAIESTKTIAV"
     gene            complement(378..>1088)
                     /locus_tag="PLN75_08565"
     CDS             complement(378..>1088)
                     /locus_tag="PLN75_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRG04207.1"
                     /translation="LNFRRSVRYFDEEKELDTEKVKRCIELATLAPNSSNMQLWEFYH
                     ITEPEMLKKMSHACLDQRATETAKQIVVFVTRRDLHHKRSKAILEFEKGNIARYSPPE
                     RQAKRWKDRQLYYSRLMPFIYSRFFGLLGVFRKVLSTSISLFRPMTTTVSESDMRVVV
                     HKTCGLAAQTFMIAMAEAGYDTCPLEGFDSRRVKKLLNLPCAAEINMVIPCGIRKGTR
                     GIWGERFRVPFEEVYKRI"
     assembly_gap    1089..1188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..3380)
                     /locus_tag="PLN75_08570"
     CDS             complement(<1189..3380)
                     /locus_tag="PLN75_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRG04208.1"
                     /translation="MELASKYNPATVEDKWYQYWLDNGFFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMQGKNACWVPGTDHASIATEAKVVARLAEKGIKK
                     TDLSRDEFLKHAWDWTHEHGGIILEQLKKLGASCDWERTAFTMDEIRSESVIKVFCDL
                     HEKGLIYRGVRMVNWDPKALTAISDEEVIHKEVNGKLYYLRYKIVESPPTPQEGDSGS
                     YGYQTSKSGSYELLKENAKVMRSFSTDAESALWQMLRGKNLGDKFRRQHIVGEYIVDF
                     VCLTKKLIVEVDGKYHDTPEMQEADNFRTNYLNSLGFNVIRFTNEEVIGDTESVLNKI
                     ENALQIPPSGGGGAFVVIATTRPETIMGDTAMCIHPENPKTAHLKGKRVIVPLINRVI
                     PVIEDEYVDMEFGTGCLKVTPAHDVNDYVLGEKYNLPSIDIFNEDGTLNEHGAMYAGM
                     DRFEVRRKIVKDLEEAGLMEKIEDYTNKVGYSERTDVPIEPKLSMQWFLKMSDLAKPA
                     LDNVENDTVKFHPEKYKNTYRYWMENIKDWNISRQLWWGHRIPAYYCTPADGGQQVII
                     VAENENLAKAKADKHPKLQGGYEMRQDDDALDTWFSSWLWPISVFDGINNPDNAEIEY
                     YYPTSDLVTGPDIIFFWVARMIMSGYEYRNQECFKNVYFTGIVRDKIGRKMSKQLGNS
                     PDPIELMKQFGADGVRMGMLLTAPAGNDLLYDDSLCEQGRNFNNKIWNA"
     gene            3595..>3921
                     /gene="recO"
                     /locus_tag="PLN75_08575"
     CDS             3595..>3921
                     /gene="recO"
                     /locus_tag="PLN75_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRG04209.1"
                     /translation="MQAKTTGIILHQVKYSDSQSIVSIYTREFGRMSYMVRGANRKKS
                     ATRSALLQPLSIVEIDVSHNPKKDIQTIKDMRIAIPFYHIPYDPVKNGLALFMTELLQ
                     KTLKHSE"
BASE COUNT          984 a          883 c          801 g         1153 t
ORIGIN      
        1 acagcaatgg tttttgtgct ttctatcgcc cgcaaacctt gctgctcagc atcgaacatc
       61 gtttttgcag aagatttgct gtttactttc agaaggaatt tttgactttg agtttccagc
      121 agatatgccg acgaaatatc accaacggaa acggggcgaa acgcagtgat ttctgattga
      181 atttgatctg aaatatgcct gatgatttgt tccatagcca acactttgat gtgaaatcgt
      241 aaagatataa aagaaaaggg atttttccta atcacaggaa aatcccttaa attttagtgc
      301 atccaacctc ttccagtcgg tttgatgcgt tattttttcg ggtcaaacgg actttgagac
      361 gttagaccct gtgggggcta aatcctctta tacacctcct caaacggcac tctaaaccgc
      421 tcgccccaga tgccgcgcgt ccctttgcgg attccacacg ggatgaccat atttatttcg
      481 gctgcgcatg gcagattgag cagttttttc acccgacggc tgtcaaaacc ttcgagcgga
      541 caggtatcgt aacccgcttc cgccatcgct atcataaatg tctgcgctgc cagtccgcag
      601 gttttatgca ccaccacacg catatcgctt tctgaaacgg tggttgtcat ggggcgaaac
      661 agactgatgg aagtggacag aacttttctg aaaacaccca acaatccgaa aaaccggctg
      721 taaataaaag gcatcaaacg gctgtaataa agctggcggt ctttccatcg tttagcctgt
      781 ctttcgggtg gactataacg ggctatgttt cctttttcaa attccagaat cgctttcgag
      841 cgtttgtggt gaagatcgcg tcgggttaca aaaacgacta tttgtttggc agtttcagta
      901 gcccgctggt ccaggcaggc atgcgacatc tttttcagca tttccggctc ggtgatgtga
      961 taaaactccc acagttgcat gttggagcta ttgggggcca gcgttgccag ttcgatgcag
     1021 cgttttacct tttcggtatc gagttccttt tcttcgtcaa aataacggac cgaacgacga
     1081 aagttcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc attccagatt
     1201 ttgttgttga aattacgacc ttgttcgcac aagctatcat cgtacagcag gtcatttccc
     1261 gcaggggcag tcaacagcat tcccatacgc acgccatccg caccaaattg tttcatcagt
     1321 tcaatcggat cgggtgagtt gccaagttgt ttggacattt tccgaccaat tttatcccgc
     1381 acaattccgg taaaatagac gtttttgaaa cattcctgat tacggtattc gtagccggac
     1441 ataatcatcc gcgccaccca aaagaaaata atatccggac cggtaaccag gtcgcttgtt
     1501 gggtaataat actcaatttc agcattatcc ggattattta ttccgtcaaa aaccgaaatt
     1561 ggccacaacc acgacgagaa ccatgtatcc agcgcatcat cgtcctgacg catttcataa
     1621 ccgccttgca gttttgggtg tttatcggct tttgcttttg ccaaattttc attttcagcc
     1681 acaataatca cctgctgacc gccatccgcc ggagtgcagt agtacgccgg aatgcggtgt
     1741 ccccaccaaa gttgacggct gatgttccag tctttgatgt tttccatcca gtagcggtag
     1801 gtgtttttgt atttttcggg gtgaaatttc accgtgtcgt tttccacatt atcaagtgcc
     1861 ggcttagcta aatcgctcat tttcagaaac cactgcatcg aaagtttggg ctcgataggc
     1921 acatcggtac gttcggaata acccactttg ttggtgtaat cttcgatttt ttccatgagt
     1981 ccggcttctt ccaggtcttt cactattttc ctgcgaacct caaaacgatc cattccggca
     2041 tacatcgctc cgtgctcgtt gagcgtgccg tcttcattga aaatatcgat ggatggcagg
     2101 ttgtattttt cgcccaatac gtagtcgttc acatcgtgcg ccggagtaac tttcaggcat
     2161 cccgttccaa attccatatc cacgtactca tcttcaatca caggaattac acggttgata
     2221 agcggaacaa tcactctttt acctttcaaa tgtgccgttt tcgggttttc aggatgaata
     2281 cacatagccg tgtcgcccat tatcgtttcg ggacgcgtag ttgcaatgac aacaaaagcc
     2341 cccccacccc ccgaaggggg gatttgaagt gcgttttcaa tcttattgag cacactttca
     2401 gtatctccaa ttacctcttc atttgtaaaa cgaatcacat taaaacccaa tgaattcaga
     2461 taatttgttc tgaaattatc agcctcttgc atttcaggag tatcatgata ttttccatca
     2521 acttcaacaa tcaatttttt agtcaaacat acaaaatcaa caatatactc acctacgatg
     2581 tgttgtcttc tgaatttatc acctaaattt ttcccacgaa gcatttgcca aagtgcactt
     2641 tcagcatctg ttgaaaagct acgcattact ttagcgtttt cttttagtaa ttcgtaactt
     2701 ccacttttcg aagtctgata gccgtagcta ccagaatccc cctcttgggg ggtggggggg
     2761 ctttctacaa ttttataacg caaatagtac aactttccat tcacttcttt gtgaataact
     2821 tcttcgtcgg aaatggctgt aagtgctttt ggatcccagt taaccatccg tacaccacgg
     2881 taaatcaagc ctttttcgtg caaatcgcag aaaactttaa tcacgctttc agaacgaatt
     2941 tcgtccatcg tgaaggctgt gcgttcccag tcgcaagatg cgccgagttt tttcagctgt
     3001 tccaggataa ttccgccgtg ttcgtgcgtc cagtcccagg cgtgtttcaa aaactcatcg
     3061 cgagaaaggt cggttttttt gatccctttt tcagccagac gagccacaac ttttgcttcg
     3121 gtagcaatgg atgcgtggtc ggttccgggt acccagcagg cattttttcc ctgcatacgc
     3181 gcacggcgca caagtacgtc ctgaatggta ttgttgagca tgtgccccat gtgaagcacg
     3241 ccggttacgt ttggtggcgg aattacgatg gtgtaaggtt cgcgctcatc gggcgtggaa
     3301 tgaaaaaatc cattgtctaa ccaatattga taccatttgt cttcaacagt tgcggggttg
     3361 tatttgcttg caagttccat gtttagtttt tagtaagcag ttaatagttt gtagtaaatt
     3421 gtaaatcaga attttacaat cacagtttat tctttcagaa tttctgattt ctgatttctg
     3481 atttctgatt tttaaaagcg caaagatagt gaaaatgaat tttttataga aatttaaatt
     3541 ggaaagaata agtattgtca gcttaattca ttaaatttgt gaaagaatag aactatgcag
     3601 gcaaaaacca caggcatcat acttcatcaa gtaaaatact cggacagtca gagcattgtt
     3661 agtatttata cgcgggaatt cgggcgaatg tcctatatgg tgcgtggtgc gaaccggaag
     3721 aaatcagcca ctcgttcggc tctgcttcaa ccgctttcta tcgtggaaat tgatgtatct
     3781 cacaatccga agaaggatat tcagaccatt aaggatatgc ggatagccat tcctttttat
     3841 cacattccgt atgatccggt gaagaatggg ctggcgctgt ttatgactga attgctgcaa
     3901 aaaacgctga aacattcgga a
//
LOCUS       DAORKP010000182         3889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6331_length_3889_cov_8.874283, whole genome shotgun sequence.
ACCESSION   DAORKP010000182 DAORKP010000000
VERSION     DAORKP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6331_length_3889_cov_8.874283"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..815
                     /locus_tag="PLN75_08580"
     CDS             108..815
                     /locus_tag="PLN75_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRG04210.1"
                     /translation="MNGIFLNEVVVVAATFIIYWLAQRLQAKTGFILLNPILITILII
                     ILFLMIFNINYEVYQKGGKYIDFLLKPAVVALGVPLYKQMKVIKKQALNIVISQLAGC
                     ITGIVSVVLIAKLMGASREIIFSLTPKSVTTPVAIEISRSIGGIAPLTASVVIVVGIF
                     GAVFGYTILKLTGVKNPMSQSLSMGNAAHAIGTSKSMEFSPAFGAMSSIGMIVNGIFT
                     AILAPYVLKLLGIWIEL"
     gene            947..2851
                     /locus_tag="PLN75_08585"
     CDS             947..2851
                     /locus_tag="PLN75_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRG04211.1"
                     /translation="MPDIHQILKTHWGYDSFRALQEDIIQSIISGKDTLGLMPTGGGK
                     SLTFQVPAMAFEGVCVVITPLIALMKDQVENLKKRNISAAAIYSGMTNTEILKTLENA
                     IFDAYKFLYVSPERLSTEIFIKKVSQMNISLIAVDEAHCISQWGYDFRPSYLRIAEIR
                     TLLPDVPVLALTATATAEVVDDIQTQLRFRQKNVFKKSFNRSNLIYVVRNAENKEEQL
                     LKILKNVPGTSVVYVRNRKKTREISEFLNQNGITSEYFHAGLKNEIKDERQARWKSDE
                     TRVIVATNAFGMGIDKPEVRTVVHMDLPDSLEAYFQEAGRAGRDEKKAYAVLLFNKSD
                     EIKLRKRIADTFPPREKVKEVYEALGSYYQIGVGSGLDRTFVFDINDFCQKFHFPILV
                     AFNAIKILQQAGYLELTDEQDSASMVLFTLKKDELYNLKNTAEQEKLIHILLRSYTGL
                     FTDLSAVNEETIASRLGWGREQLYLQLTELANQRVIKYIPRRKTPFLTFVQERESTER
                     LFLSTVVYDERKERYTKRVKSVLEYAKEENLCRNQILLSYFNETNSKPCGKCDVCLRK
                     MEKELTNEEFLKIKNGVESLLSSNECSVGKLVEALPFREEKIVKVLRYLLDNQYIIYD
                     EVMKLKWVKK"
     gene            2953..3876
                     /locus_tag="PLN75_08590"
     CDS             2953..3876
                     /locus_tag="PLN75_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRG04212.1"
                     /translation="MKVYKENKLRYFRWHWGDRYDAWRVRKVDPVFSVIPYFLRTRMD
                     AQNYFEERIPIDNIEAFIREHKEEIPGLSLMHVVIAALVRLISQRPNLNRFVMWNKIF
                     ARNYLNISIAVKRTLSDEGEETLIKPYFLPTDTLYDVVKKIKKEQEENQQVGQQNTSD
                     MISKILGVLPDFMMRIVVFSLLWMDKVGLLPRFIEQASPWHCSFFLTNIGSIGVESIY
                     HHLYEFGNCSIFVALGKKSQRHTVSRSGDVKPYKSILLKFVLDERICDGYYYASSMRT
                     LAKIFQNPEQLLTPPEKVLVDEGVKWKRIDI"
BASE COUNT         1190 a          792 c          859 g         1048 t
ORIGIN      
        1 gcttcggccg taagtacggt attggtgctc attgttacag gctggactca tcagcttatc
       61 cgcaataaaa gcagaaaaac ccgtgataat attagtaccg acgacttatg aacgggatat
      121 ttctaaatga agtggtcgtc gtagcagcta cgtttatcat ttactggctg gcgcagcggc
      181 ttcaggcaaa aacaggtttt attttgctca atcccattct tatcaccatt ttgattatca
      241 tcttgtttct gatgattttc aatatcaatt atgaagtgta ccaaaaaggt ggtaagtata
      301 tagattttct gctcaaaccg gcggtggtag ccttgggtgt gccgctttac aagcaaatga
      361 aagtaatcaa aaaacaggca ttaaatattg ttatttcgca gttggcaggc tgcattacag
      421 gaattgtatc ggtagtgctc attgcaaaac tgatgggcgc atcgcgcgaa attatttttt
      481 cactgactcc caaatcggtt accacacccg ttgccataga gatttccaga tccattggcg
      541 ggatagcgcc gctcacggca tcggttgtga ttgtggtggg aattttcgga gccgtattcg
      601 ggtacaccat tcttaaactt accggagtga aaaatccgat gtcccaaagc ctgtcgatgg
      661 gaaatgctgc tcatgccatc ggcacttcca aatccatgga attcagtcct gcgtttggag
      721 ccatgtcaag catagggatg attgtcaacg gtattttcac cgctatactt gccccctacg
      781 tattgaaatt actcggaatc tggattgaac tttaattcag gttttttttc ttcactactc
      841 agagccttca atcacttaga aggctcaaaa aatcagaact tgtactctca ttttcggtat
      901 tttcgggata ttttgctaaa tttgtatttc tttgcacctg aaaataatgc cggatattca
      961 ccaaatactc aaaacacact gggggtacga ttcgtttcgt gcgcttcagg aagatatcat
     1021 tcaaagcatc atctccggta aagataccct tggcctgatg cctaccggag gcggaaaatc
     1081 gctcactttt caggttccgg ccatggcttt cgaaggtgtt tgcgtggtta tcacaccgct
     1141 tattgcattg atgaaagatc aggtggagaa cctcaaaaaa cgcaacatca gtgcggctgc
     1201 aatctattcg ggcatgacaa acaccgaaat tctgaaaaca ctcgaaaacg caatttttga
     1261 cgcctataaa tttctctatg tttcacctga aaggctcagc actgaaatat tcataaaaaa
     1321 agtttcccaa atgaatatca gcctgatagc ggtagatgaa gcacattgta tttcgcagtg
     1381 gggatatgat ttcaggcctt cttaccttcg gatagccgaa atccgtacgc tgcttccgga
     1441 tgtgccggta ctggcactta cggctacggc tacagccgaa gtagtggacg atattcagac
     1501 acagcttcgt ttcaggcaga aaaacgtgtt caaaaagagt tttaatcgtt ctaacttaat
     1561 atatgtggta cgaaatgccg aaaataagga agaacaactc cttaaaatat tgaaaaatgt
     1621 acccgggaca tcggtggtgt acgtacggaa ccggaaaaaa acacgggaaa tttcagagtt
     1681 tctgaatcag aacggcatca catctgagta tttccatgcc gggcttaaaa atgaaatcaa
     1741 agacgagcgt caggcacgct ggaaatcgga cgagacgcga gtcattgttg ctacaaatgc
     1801 gtttggaatg ggcatcgaca aacccgaagt acgcaccgtg gtgcacatgg acctgcccga
     1861 ttcgctcgaa gcgtattttc aggaagcagg ccgggccgga cgcgacgaga aaaaagccta
     1921 tgcagtgctg ctgttcaata aatcagacga aataaaactc cgtaaacgta tagctgatac
     1981 ttttccaccc agggaaaaag taaaggaagt atatgaagca cttggatcgt actatcagat
     2041 aggagtaggt tccggactgg accgtacttt tgtgtttgat atcaacgatt tctgtcagaa
     2101 atttcatttt ccaattttgg tggcattcaa tgccattaaa atactccagc aagccggtta
     2161 cctggaactt accgacgagc aggacagtgc gtccatggtt ctgtttaccc tgaaaaaaga
     2221 tgaactttac aacctgaaaa atacagccga acaggaaaaa ctgattcata tcctgctacg
     2281 ttcttacacc ggtttgttca ccgatttgag cgctgtcaac gaagaaacca tcgcttcgag
     2341 gctggggtgg gggcgcgaac agttatactt gcaactcacc gaactggcca atcagcgtgt
     2401 aattaagtac attccacgca ggaaaactcc attccttacc ttcgtgcagg aacgtgaaag
     2461 taccgaaagg ctgtttctca gcaccgtggt ttacgatgag cgaaaagaaa gatacaccaa
     2521 aagagtgaaa agtgtgctcg aatatgccaa agaagaaaac ctttgcagaa atcagatttt
     2581 gctaagctat ttcaatgaaa cgaattcaaa gccttgcggc aaatgcgacg tttgtctgcg
     2641 aaaaatggaa aaggaattaa ctaacgagga gtttctgaaa atcaaaaatg gggtagagag
     2701 tttattgagc agcaatgaat gctcagtcgg caaactggta gaagccttgc ccttcaggga
     2761 agagaaaatt gtaaaagtgt tgcgatattt attagataat cagtatatta tatacgacga
     2821 agtgatgaaa ttgaaatggg tgaaaaaatg aaaatttacg accgattata aataaataaa
     2881 aaatcaattc ttattttcag aaaagataga ttatctttga gaccttttta tatacattaa
     2941 attatcaata ccatgaaagt ttacaaagaa aataaacttc gctatttccg ctggcactgg
     3001 ggcgacaggt acgacgcctg gcgtgttcgc aaggtcgatc cggttttttc ggttattcca
     3061 tacttcctcc gaacccgcat ggatgcacag aattatttcg aagagagaat tcctattgac
     3121 aacatcgaag cgttcatccg cgaacataag gaagaaatcc ccggtctttc gttgatgcat
     3181 gtagtgatag cagcacttgt aaggctcata tcgcaacgtc cgaatctgaa ccggtttgtg
     3241 atgtggaaca aaatctttgc gcgaaattac ctgaacattt ccatagccgt aaaacgaaca
     3301 ctctcagacg aaggagaaga aacgctgata aaaccctatt ttctgccaac agatacattg
     3361 tatgatgtag taaaaaaaat aaaaaaagag caggaagaaa atcagcaggt tggtcagcag
     3421 aatacatcgg atatgatttc aaaaatactg ggtgtattac ctgattttat gatgagaatt
     3481 gtcgtattct ctctcttgtg gatggataaa gtcggacttt taccacgctt cattgagcag
     3541 gcaagcccgt ggcattgcag cttctttctg accaatatcg gttcaattgg cgtagaatcc
     3601 atttaccatc atttgtacga attcggcaat tgcagcatct ttgtggcgtt aggtaaaaaa
     3661 tcgcagcgtc atacagtgag ccgctcagga gatgtaaaac cgtataaaag cattttgctg
     3721 aaatttgtcc tcgatgaacg tatctgcgac ggatattact atgcttcgtc catgcgtacg
     3781 ctggcaaaga tattccagaa tcctgaacaa ttgcttactc cgcccgaaaa agtattggtg
     3841 gacgaaggtg tgaaatggaa gagaattgat atttagtcga atagtcgaa
//
LOCUS       DAORKP010000183         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6851_length_3730_cov_9.581769, whole genome shotgun sequence.
ACCESSION   DAORKP010000183 DAORKP010000000
VERSION     DAORKP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_3730_cov_9.581769"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="tyrS"
                     /locus_tag="PLN75_08595"
     CDS             complement(<1..365)
                     /gene="tyrS"
                     /locus_tag="PLN75_08595"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRG04213.1"
                     /translation="MNFIEELEWRGMIHTVMPGTDEQLKKEMTTAYVGIDPTADSLHI
                     GHLVSVMMLRHFQRAGHKPVALVGGATGMIGDPSMKSAERNLLDEETLRHNQECIKAQ
                     LAKFLDFESNQPNAAELVN"
     gene            540..1928
                     /locus_tag="PLN75_08600"
     CDS             540..1928
                     /locus_tag="PLN75_08600"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRG04214.1"
                     /translation="MRKYLFLVFILLLCWSCRDEVPEVVVDPPAPKDTVTGKTGSAYV
                     FDIEKVPEVYIETSTVEWNKLLSYFDQNPYNEEFIRADFVFVKEGRRDTLENIGLRLR
                     GNTSRRRPEGKKGELHKSENPDWHHSHFSVNLNKYVKGQSLSGTQKITLKWFKDDGVY
                     AREVYCYDLFERFGVWTAPQSSYCRLFLKIKEDEKWINYGVYELLEPVDNDFIENRKK
                     HFQGSSGNLWKANWGADLVNPEKSRMGIEQVTLSSTYRPVYDLKTNETDLETARNQLA
                     GFINQFNSKQGDDFKIWIATATDVPLLLKTYAVSTLCGMWDDYWNNSNNFYFYFDAND
                     KFYFIPFDYDNTLGTSLLMPDAGRQDLLNWGKSSNPMVKKILAFSEYRELYISYLNEL
                     CDPANDLFYYTRSIDRIQKWHDMIRYYITNDTGEDMEIKDVPAGWGNCPHYRLLNASN
                     NYFVIRAQNLPK"
     gene            complement(1983..2540)
                     /locus_tag="PLN75_08605"
     CDS             complement(1983..2540)
                     /locus_tag="PLN75_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04215.1"
                     /translation="MKKNLSKIGFMALILAASTLAFTACNKKAESTESTEMAESDSIV
                     IVEEIPETKDYSAFTFEQKNEVIADAKAELDVLNKKIDDLKAEAKDTKKELSVDVKSN
                     YEKAIKDLEKSRDEYKQKVDALEKSTAETWEAAKTDIANAYNTAAQGIENTLTNTKSK
                     VTGAVEDAKEAVNKGIDSLQNKILK"
     gene            2771..3517
                     /locus_tag="PLN75_08610"
     CDS             2771..3517
                     /locus_tag="PLN75_08610"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRG04216.1"
                     /translation="MKFKPLLFVVALAAFSCAPKQWVITGSQTAAIPIDSTTQALADQ
                     QMIDFITPVKQNLDKEMNQVIAHTAEEMRAGKPESLLSNWSADVYLYAASEFLKSPVD
                     MAVVNMGSLRALLPKGDITVGNIFQLMPFENELVVLWLRGKDVKRLLDIFAAEGGQGV
                     AGVRMQIKDGKAVNCTIQGKPIDDETLYSIATNDFLAGGNDRMIPLASPVKRVDPGLK
                     IRNILMEYVIRETHEGRKIQSKLDGRISVL"
BASE COUNT         1002 a          741 c          846 g         1141 t
ORIGIN      
        1 ttattcacca gttcggcagc gttcggttga ttgctttcga aatcaagaaa tttggccagt
       61 tgtgctttga tgcactcctg gttgtgacgc aatgtttctt catcgagcag gtttcgctcg
      121 gccgatttca tggatgggtc acctatcatt ccggtggcgc ctccaaccag agccacgggt
      181 ttgtggcctg cccgctgaaa atgccggagc atcatcacac tcaccaaatg gccgatatgc
      241 agtgaatctg ccgtggggtc gataccaacg taggcagttg tcatttcctt ctttagttgt
      301 tcatcagtgc cgggcattac ggtatgaatc atgccacgcc attcaagttc ttctataaaa
      361 ttcatctgat tgagtatttt ttatttcggg tgcaaagata gctaattttg ggtaaatcat
      421 aaagatgcag tttgtaaata aaacagttaa aaaactgctt gtttgatgtc cgtatatttt
      481 cacaatccta taattttgac ttactttgtc tggtaaaaaa atgatttacc cgttatctga
      541 tgagaaaata tctttttttg gttttcattc ttctgttgtg ctggtcgtgc cgggatgaag
      601 tgccggaagt ggtcgtagat ccgcccgcac cgaaagacac tgtgaccggt aaaacgggtt
      661 cagcgtatgt ttttgatatt gagaaagttc cggaagtgta tattgaaaca tcgactgtgg
      721 aatggaacaa actcctcagc tattttgacc agaatcctta caacgaagag tttatcaggg
      781 ccgattttgt gtttgtaaaa gagggccgac gtgatacgct tgagaatata gggcttcggc
      841 tgcgtggcaa tacaagccgg cgtcgtcctg aagggaagaa gggagaactc cataaatccg
      901 aaaatccgga ctggcatcac agccatttct ccgttaatct gaataaatac gtgaaagggc
      961 aaagtttgtc cggcactcag aaaatcacgc tgaaatggtt caaagacgac ggagtgtatg
     1021 cccgcgaagt gtattgttac gatctttttg agcgtttcgg tgtatggact gccccgcaat
     1081 cgagctattg ccgtctgttt ctgaaaataa aggaagatga aaagtggatc aattacggtg
     1141 tgtacgaact gttggaacct gtggataatg attttattga aaacagaaag aaacattttc
     1201 agggttcgtc gggcaacttg tggaaagcca actggggagc cgatttggtg aatccggaaa
     1261 aatcgaggat gggaattgaa caggttacgc tcagttccac gtatcgacct gtatatgatt
     1321 tgaaaaccaa cgaaactgac ctggaaactg cgcgtaatca gctggccggt tttatcaatc
     1381 agtttaattc aaaacagggt gacgatttca aaatatggat agcaaccgct acggatgttc
     1441 ccctgttgct caaaacctat gctgtaagca ctctctgcgg catgtgggac gattactgga
     1501 ataattcaaa caatttctac ttctatttcg atgccaatga taagttctat ttcattccgt
     1561 ttgattatga caatacgctg ggcacttcgc tgcttatgcc tgatgcgggc aggcaggatt
     1621 tgctcaactg ggggaagtca tccaatccga tggtgaaaaa gattctggct ttttcggagt
     1681 acagagaact ttatatcagc tatttaaacg agctttgcga tccggccaac gatctgtttt
     1741 attacacccg aagcatcgat agaattcaaa aatggcacga tatgatacgg tattacatca
     1801 ccaacgatac cggcgaagac atggagatta aagacgttcc ggccggttgg ggcaactgcc
     1861 ctcactacag attgctcaac gcttccaaca actattttgt gatcagagct caaaatctac
     1921 ccaaataaaa aaaggagcac tgaagctcct tttttctaca ttttacattt caaccggtag
     1981 ttttatttca ggattttgtt ctgcaggctg tctattcctt tattaacggc ttctttggca
     2041 tcttccactg cgccggtcac tttcgatttg gtgtttgtga gcgtgttttc aatgccctga
     2101 gctgctgtgt tgtaagcgtt tgcaatgtct gttttggcag cttcccaggt ttcggctgtg
     2161 cttttttcga gcgcgtccac tttttgtttg tattcatcgc gcgatttttc aaggtctttg
     2221 attgcctttt cgtagtttga ttttacatcg accgaaagct cctttttcgt gtccttagct
     2281 tctgccttga ggtcgtcaat ttttttattc aatacatcca gctctgcttt tgcgtcagct
     2341 atcacttcgt tcttttgctc aaacgtaaag gcgctgtaat ctttggtttc cggaatctct
     2401 tccactatga caatagaatc gctttcagcc atttcggttg attcggttga ttctgctttt
     2461 ttattgcaag cagtaaaagc taaagttgat gctgcaagga ttaaagccat aaatccgatt
     2521 tttgagaggt tctttttcat tgatatgatt ttttaattag ttaaatagtt gtttacttta
     2581 ttaaacaaat aatgtgctaa aaatgttttc tgaataataa aattaattaa aattaacgct
     2641 cgataggtgt tttgatgaca atatcgtgtt ctgataactt cgaatcaaac gattttttct
     2701 caatttgtca tgcgtttttt gtaaaaataa atctatattt gtagttctat aaaacaaaat
     2761 gattttggat atgaaattca aaccactgtt atttgtcgtt gcattagcag cattttcgtg
     2821 cgctccgaaa caatgggtaa ttaccggttc gcagacggct gctattccga ttgattccac
     2881 cacacaagcc ctggccgacc agcaaatgat tgactttatt actcctgtaa agcaaaatct
     2941 ggacaaggaa atgaatcagg taatagccca tactgccgaa gaaatgagag ccggtaagcc
     3001 tgaaagtttg ttatccaact ggagtgcaga tgtgtatctc tatgctgcgt ccgaattttt
     3061 gaaatctccc gtggatatgg cggtggtaaa tatgggaagt ctccgggcgc tgctgcccaa
     3121 aggcgatatt acggttggca atatttttca gcttatgcca tttgaaaatg agctggtagt
     3181 gctctggctc cgtgggaagg acgtgaagag gctgcttgac atatttgcgg ccgaaggcgg
     3241 acagggagtt gcaggtgtga ggatgcaaat aaaggacggt aaagccgtga attgcacgat
     3301 tcagggaaaa cccatcgatg atgaaacact ctacagcatt gccaccaacg attttctggc
     3361 gggaggcaac gaccggatga taccactcgc ttcacccgta aaaagagtgg acccgggtct
     3421 gaaaatacgc aatattctga tggaatacgt tatccgcgaa acgcacgaag gacgcaaaat
     3481 ccagtcgaag ctggacggac gaattagcgt gctctgaagt gtatttgaga tctgattgaa
     3541 aaatcaaaaa gagagtcgct ttttaaaatt gcaactctct tttttttttg cgttagaaat
     3601 ctgaaaagac gcttcaaagg tgttacttgc taaatccgtg aagtcttgat gatggttccg
     3661 tcttcgagat aaaactcgat gcctacgagt ccgaatttgt gattgccgat gtaaacaatt
     3721 tttgtaattc
//
LOCUS       DAORKP010000184         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6894_length_3719_cov_7.343614, whole genome shotgun sequence.
ACCESSION   DAORKP010000184 DAORKP010000000
VERSION     DAORKP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_3719_cov_7.343614"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1456)
                     /gene="nhaA"
                     /locus_tag="PLN75_08615"
     CDS             complement(131..1456)
                     /gene="nhaA"
                     /locus_tag="PLN75_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRG04217.1"
                     /translation="MANRNTYPIDKLTLPVQNFIQKEKSGGIVLGITVLVALYLANSP
                     WSYQYFHFLENKLGFHFNDVSFLKYSLHHWINDGLMSIFFFVVGLELKREIVAGELSN
                     PRKALLPIGAALGGMLVPAVIYIALNPAGEASNGWGIPMATDIAFALGVLHLLGKRIP
                     LTLKIFLTALAIVDDLGAVLVIAFFYTSDISFVSLLTGLGFVFVMFIGNKMGVRNILF
                     YAIVGIAGVWIAFLLSGVHATIAAVLAAFTIPSNVGINEGIFIKRINASLDKFKKIEP
                     NDKVTLTGEQIHQLETIKADTNCAIPPLQRLEHSMHPFVTFFVLPVFALANAGVSLDI
                     DVQALLSTNVALGVGLGLLVGKVAGIVGFTMLLVKLKVAPFPDGMNVRNLFGIGLLAS
                     IGFTMSLFITSLAFTSESLITQAKIGIFVASIIGGLLGYFLLRTGKKQS"
     gene            complement(1586..2821)
                     /gene="xseA"
                     /locus_tag="PLN75_08620"
     CDS             complement(1586..2821)
                     /gene="xseA"
                     /locus_tag="PLN75_08620"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRG04218.1"
                     /translation="MNSTSLSELTNRIQAVIRHNFTQAIWVRAEISELRENGGHCYLE
                     LIEKDSETDNILAKTRATCWASVYRMLKPYFESSTGETLRAGLNVLVAVTVEFNGVYG
                     FSLNVRDIDPTFTIGELAARRMQIILKLREEGIDDMNKQLPFPVLPQRIAIISSETAA
                     GYGDFYNQLNSDTSNYVFYPHLFPAVMQGNQAEASIIGALEKIYKHVELFDVVVIIRG
                     GGATTDLSCFDSYNLALNCAQFPLPIISGIGHQRDVSILDMVAHTSVKTPTAAAEFLI
                     SALEKAEEEIDELASDLYNATSTIIQAKLKSLELITLRIKQASKTGYLKEQNRLSGYL
                     FQLKNRLQIRIQKEKNRLTLIETKIETQSPTYLYARGYTITTLNGKRITSAHQVKPGD
                     KVTTHLNDGRFDSEVVPLL"
     gene            complement(2818..>3719)
                     /locus_tag="PLN75_08625"
     CDS             complement(2818..>3719)
                     /locus_tag="PLN75_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRG04219.1"
                     /translation="LLIRTESAEGEYRCEPDFWISGIEYADSAGVDLATTSLGYTTFD
                     DPAMNYTYADMNGKTARASIAATMAFKKGIMLLNAAGNDGNKTWKYIGVPGDAEGVIT
                     VGSVRKDSVYSAFSSIGPSADGRIKPELAALGTSTYVISTSGSTANASGTSFATPVLA
                     GITACYLQAAKSLKPHLTLPEIQNNLFKTGHLYNNPTAETGYGIPDFQKAFYELTSNT
                     VSQTQANQLSQKKAIKLLVDDTKRNILIKLNTQNHTEIGIVRVFSLTGQIVSEKSYSG
                     SYIQINTEKLEKGIYLIQINTER"
BASE COUNT         1006 a          821 c          820 g         1072 t
ORIGIN      
        1 aaaaatatta aggaaaaaat agctcattat gccgtaagaa atccaacaag gataacctca
       61 cttacgtttc gaacagacag aggaaaaaaa ccttgaaaga aaaataatat ttttaagaaa
      121 agcaggttgc ttatgattgt tttttccctg ttctcaataa aaaatagccc agcaagccac
      181 caataatgga tgccacaaaa atgccgattt tagcctgagt aatcagagat tcgctggtaa
      241 atgccagtga agtaatgaaa agcgacatgg taaagccgat agaagccagc aatccgatac
      301 caaacagatt ccttacgttc attccatccg ggaagggtgc cactttgagt ttcaccagaa
      361 gcatcgtaaa tccgacaata cctgccactt taccaaccag aagtcccaac ccgacaccca
      421 gcgcaacatt tgtactcaaa agtgcctgaa catcaatatc cagcgatact ccggcgttgg
      481 ccagcgcaaa aaccggcagt acgaaaaaag ttacgaacgg atgcatggag tgttccagtc
      541 gctgaagtgg tggaatggca caatttgtat ctgccttaat cgtttcaagc tgatgaattt
      601 gttctccggt taaggtcact ttatcgttag gttctatttt tttgaattta tcaagcgatg
      661 cattgattcg tttgatgaaa atgccctcat ttattcccac gttgctggga atggtaaatg
      721 ctgcaagcac cgcagcaatt gtggcgtgca cgcccgatag caaaaaggca atccaaacgc
      781 ctgcaatgcc aacaatggca taaaacagaa tatttcgcac gcccatttta ttgccgataa
      841 acatcacaaa cacaaatcct aatccggtga gcaaactcac aaaagaaata tccgatgtgt
      901 agaaaaatgc aattacgagc acagcgccaa ggtcgtcgac aatggccagg gccgtcagaa
      961 aaatcttgag agtgagcgga atccgcttgc cgagcaggtg aagcacaccc agtgcaaacg
     1021 caatatcggt agccatcgga attccccagc cgttgctcgc ttcgccagcc ggattcaaag
     1081 caatgtaaat cacagcaggc accagcatac ctccaagggc agcaccaatg ggcagaagcg
     1141 ctttgcgcgg attagaaagc tcaccggcaa cgatctcacg tttgagttcc agccctacca
     1201 caaaaaagaa aatggacatc agcccgtcgt ttatccagtg atgtaaactg tatttcaaaa
     1261 aggaaacatc attaaaatga aagcccagtt tattttccag aaaatgaaaa tactgataag
     1321 accacggtga attggccaga taaagtgcca ctaatacggt gattccaagc actattccac
     1381 ccgatttttc cttttgaatg aaattctgaa caggcagggt gagtttgtca atcggatatg
     1441 tgtttctgtt tgccattttt ttttgaaaaa gtagtcagct gatttgagtt attaaatatg
     1501 ttaacagcta agcatagaaa gatgtttttc tgttgcttag agatttgtaa ataaatcaaa
     1561 tattttaaca aaatcaatct gtttgctaca atagtggcac gacttcactg tcaaatcgtc
     1621 cgtcattcag gtgtgtggtt actttgtcgc ccggttttac ctgatgagcc gaggtgattc
     1681 gttttccgtt cagcgtggtt atcgtgtagc cacgcgcata gaggtacgtt ggcgattggg
     1741 tttctatttt ggtctcgatg agggttagcc tgtttttttc cttttgaatg cgaatttgca
     1801 accggttttt aagctggaat aaatagcccg aaagtcggtt ttgttctttt aaatacccgg
     1861 ttttggaagc ttgcttaatt cttaacgtaa ttaattccag tgatttaagt ttcgcctgaa
     1921 tgatggtaga tgttgcattg tacaagtcag aagctaattc gtctatttct tcttctgcct
     1981 tttcaagtgc ggaaatcaaa aattctgctg ctgctgttgg tgtttttaca ctggtgtgag
     2041 ctaccatatc caaaatggaa acatcgcgct gatgcccgat gccggatatt attggcagag
     2101 gaaactgagc gcagttcaaa gccaggttgt aggagtcgaa gcacgaaaga tcggttgtag
     2161 cgcctcctcc gcgaataatt actactacat caaacagttc cacgtgtttg taaatcttct
     2221 ccaatgctcc aatgattgat gcttctgcct gattcccctg catgacagcc gggaaaaggt
     2281 gcgggtagaa aacatagttt gaggtgtcag aattcagttg attgtaaaaa tcgccgtatc
     2341 cggctgctgt ttccgacgaa ataatagcaa tccgctgtgg gagaacaggg aagggaagtt
     2401 gcttgttcat atcatcgatt ccttcttccc tgagtttcag aatgatttgc atccgcctcg
     2461 ctgccagttc gccgattgtg aatgtaggat ctatgtccct tacattcagg ctaaaaccat
     2521 aaactccgtt gaattcaacc gtcacggcta ccagcacatt cagtcctgcc cgcagcgttt
     2581 cgcccgtact gctttcgaag taaggtttca gcattctata cacgcttgcc cagcaggtgg
     2641 cgcgcgtttt ggccagaata ttatcggttt cgctgtcttt ttcaatcaat tccaggtaac
     2701 aatgacctcc gttttcgcgc agctcgctaa tctcggcacg cacccagatt gcctgagtga
     2761 aattatgccg gataacggcc tgaatccggt tggtgagttc ggatagtgag gttgaattca
     2821 tcgttccgta tttatttgaa tgagataaat tcctttttcc agtttttccg tatttatttg
     2881 aatataagac ccggagtaac ttttttctga aacaatttgt cctgtcagtg aaaatacgcg
     2941 gacaattccg atttcagtgt gattttgggt attcagtttt atcagaatat ttctcttcgt
     3001 atcatctacc aacagcttga ttgctttctt ttgggataat tggttcgcct gagtctgact
     3061 caccgtattt gatgttaatt cgtagaatgc tttctgaaaa tcgggtattc catatcccgt
     3121 ttctgcggtc ggattattgt acaaatgacc tgttttgaac agattattct gaatttccgg
     3181 cagagttaaa tgtggtttga gtgattttgc ggcttggaga taacaggctg taatccctgc
     3241 cagcacaggt gtggcaaagg atgttccact ggcgttggca gttgatccgg atgtgctgat
     3301 cacgtaagtt gacgtgccga gtgctgcaag ttccggtttg atgcgtccgt ctgctgaggg
     3361 tcctattgac gaaaaagcac tgtaaacact gtctttccgt accgatccca ctgtaatcac
     3421 tccttcggca tcgcccggaa ctccgatgta tttccacgtt ttgttgccat cgttgccggc
     3481 tgcattgagc agcatgattc cttttttaaa tgccattgtg gcggctatgc ttgcccgggc
     3541 agttttcccg ttcatatcgg catacgtgta gttcatggcc gggtcatcga atgtagtata
     3601 ccccagcgat gtagtcgcta aatcaacacc tgcgctgtct gcatattcta ttccggaaat
     3661 ccagaaatcg ggttcgcacc ggtattctcc ctcggccgat tcggttctga taagcaggt
//
LOCUS       DAORKP010000185         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_6987_length_3692_cov_7.858675, whole genome shotgun sequence.
ACCESSION   DAORKP010000185 DAORKP010000000
VERSION     DAORKP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6987_length_3692_cov_7.858675"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..589)
                     /locus_tag="PLN75_08630"
     CDS             complement(272..589)
                     /locus_tag="PLN75_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRG04220.1"
                     /translation="MNIYVSNLNYSSSSDSLQELFAGFGEVTSASIITDRETGRSRGF
                     GFVEMPNDAEGQKAIDELNGTDFEGKTLNVNVARPKTERSSGGGYNSNRGGNGGGYNR
                     RRY"
     gene            complement(648..1073)
                     /locus_tag="PLN75_08635"
     CDS             complement(648..1073)
                     /locus_tag="PLN75_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRG04221.1"
                     /translation="MNKKELEKKKQSKKQEKLKRKENRKTNSGTKSFDDMIAYVDEHG
                     NITDTPPDREKTAKIELESIAISTPKKEDVEYTPLNGRVEYFNTEKGYGFIRDVNSSE
                     KYFFHINNAPASIAEGNQVTFDAERGKIGMNAINITLVK"
     gene            complement(1098..2219)
                     /locus_tag="PLN75_08640"
     CDS             complement(1098..2219)
                     /locus_tag="PLN75_08640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRG04222.1"
                     /translation="MTFKELNITEPILKALLDKNYEIPTPIQVQAIPVALSGKDLLGI
                     AQTGTGKTAAFAIPIIQNLLTVSNNRKRGIKALILTPTRELAIQIDECFTEYAKFTEL
                     KNTVIFGGVNQRSQVDELKRGTDILTATPGRLLDLIAQKYIQLDSLQHFVLDEADRML
                     DMGFIHDIKRLIPMLPKKRQTLFFSATMPSAIATLSKSILVNPVRVEVTPESPAAETV
                     EQHVYIVEKTQKSELLINLLNQKRNQSVLVFSRTKHGADKIARILNKKGINCEAIHGN
                     KTQNARQRALTNFKSGKTSVIIATDIAARGIDIVNLELVINYDMPDVAETYIHRIGRT
                     GRAGQNGTALTFCTAEEKAMVKDIQKLTGKKLNELAIPA"
     gene            2885..>3692
                     /locus_tag="PLN75_08645"
     CDS             2885..>3692
                     /locus_tag="PLN75_08645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRG04223.1"
                     /translation="MKKLTKEDISQEMFDLYDDYAHNKIDRRQFIKDLSIFAIGGLSL
                     PSLLSFMTPNYVDTMTIKPEDPRLISKFITYDSPKGGGTIKGLLSKPSKTRKKLPGII
                     IVHENRGLNPYIEDVGRRAALEGFITLAPDALSPLGGYPGNDDAGRELQKKRSSEEML
                     EDFIAAYEYLKSHKNCNGYVGVVGFCFGGWISNMMAVKIPDLPAAVPYYGGQPTAVEA
                     EKISNPIMLHFAGLDTRVNEGWPAYEEILKKNKVEYTAYFYEGVNHGFHNN"
BASE COUNT          985 a          685 c          737 g         1285 t
ORIGIN      
        1 aaaattaatt aaattaaatc gtaagactta agacactact tcagtcaatt ttacctatta
       61 aacaaataag gcggaaaaaa gttttggaga aaaataaaaa aatcaattta aaaaatcaga
      121 aaattcagaa tgtgctgaaa ttcaaattgt tgatatttat tataaaatta ttgctgataa
      181 ttttacaaat caaacccaaa aacaaaccgc acattgttaa atgcgcggtt ttttggtgtg
      241 atcataaagc tgcctgggta agaaattacc gttagtatct tcttcggttg tatccgcctc
      301 cgttgccgcc gcggttgctg ttgtatccgc cgccgctgct tctttcggtt ttcggacggg
      361 ctacgttcac gttgagtgtc tttccttcga aatctgttcc gttcagttcg tcgattgctt
      421 tttggccttc agcgtcgtta ggcatttcta cgaagccaaa gcctcttgag cggccggttt
      481 cccggtctgt gatgatactg gcagatgtta cttctccaaa acctgcgaat aattcctgta
      541 agctgtctga tgaagagctg tagtttaaat ttgatacgta aatgttcatt ttttcttttt
      601 ttaaaaattg tttattaata agttggttta tttatttgtg tcgttttcta ttttacaagc
      661 gtgatattta tggcgttcat gcctattttt cctctttctg catcgaaagt cacctgattg
      721 ccttctgcaa ttgatgcggg ggcattattg atgtggaaaa aatacttttc ggatgaattt
      781 acatccctga tgaaaccata ccctttttct gtgttgaaat attctacccg gccgttgagc
      841 ggtgtgtatt ctacatcttc ttttttgggt gtggatattg caatgctttc aagctctatt
      901 tttgctgttt tttcacgatc cggcggagta tcggttatat ttccgtgttc atctacatag
      961 gctatcatat catcaaaaga cttggtgccg ctgtttgttt tccggttttc tttccgttta
     1021 agtttttcct gctttttgct ctgttttttc ttttctaatt cttttttatt catgtttata
     1081 gtgtgtttgt ctttttttta tgccggaatg gcaagttcat tcagtttttt acccgtaagt
     1141 ttctgaatgt ccttcaccat tgctttctct tcggccgtac agaaagtcag cgctgttccg
     1201 ttttgacctg cacgtccggt acgtcctatg cggtgaatgt aggtttcggc cacatcgggc
     1261 atgtcgtagt tgattacaag ttccaggttc acaatgtcga taccgcgtgc cgctatgtcg
     1321 gtagcaatga ttacactcgt ttttccggat ttgaaattcg taagggcacg ttgccttgca
     1381 ttctgggttt tgttgccgtg aatggcttca cagtttatcc cttttttgtt cagtatccgt
     1441 gctattttgt cggctccgtg tttggttcgc gagaatacca gtaccgattg atttcttttc
     1501 tggttcagca ggttgattag caattcgctt ttctgcgttt tttcaactat atacacgtgt
     1561 tgctctacag tttcggcagc cggcgactca ggcgttactt ccacccgaac cgggtttacg
     1621 agtatcgatt ttgaaagcgt ggctatggcc gaaggcattg tagctgagaa aaacaacgtt
     1681 tgcctctttt tcggaagcat cggtatcagc cgtttgatat cgtgaataaa acccatgtcg
     1741 agcatgcggt cggcttcgtc gagtacaaag tgctgaaggc tgtccaactg aatgtacttt
     1801 tgtgcaataa gatcaagcag tcttcccggt gtagctgtca ggatgtcggt tccccgtttt
     1861 aattcatcta cctgcgaccg ctgattgaca cctccaaaaa taacggtatt tttcaattct
     1921 gtaaatttgg catattcggt gaaacattcg tctatttgta tagccagctc ccgggtagga
     1981 gtaagtatca acgctttaat accccgtttc cggttgttgc ttactgtcag cagattttgt
     2041 ataatcggaa tggcaaaagc ggctgtttta cccgttcctg tttgagcaat acccagtaaa
     2101 tctttaccac tgagggctac aggaatggcc tgaacctgaa tgggagtggg tatttcataa
     2161 tttttatcca gcagagcctt taatatcggt tctgtaatat ttaattcttt gaatgtcatt
     2221 attttatact gtagctatac tgtattttta ttaaaagttt atgtcgcgcc ggtattgtat
     2281 agcttgattt gtaatttctc ttccggcagg taaaagaata accatactac cctgaaaaac
     2341 ggatagctgc cattttttcc cccgattaaa atttgatttg tacatctgaa aaaagagtgt
     2401 gtcacgatac ttgtgtaaca cacactccgc gacctgttgt cttaacaaga aagctgattt
     2461 tgaggtcgat tttgacgatg aatggacttg cccgttgaag gcaaagcaaa gcatggctgt
     2521 gatggaaatc aatataaact tgattgttca ggttgcaaag atacgatttt tttagtgatt
     2581 atcaattatt tttattttat ttgttaattc aaagatatgg aaaatgcgta gcacatcttg
     2641 aactgttttt cttcagtttg ttttgactta ttgatcatat ttcagaattt attctaaaat
     2701 agcataacaa tctcatcttc attctcaaat tgatataaat gttttgacct ttaaaattaa
     2761 attctttact ttgtgtttac ataagttcct gatttttatg ttttaagttt gatttctcaa
     2821 aataataata cccccggaac taatgctttt atcatcgctt attcatttaa taaacacagg
     2881 aattatgaaa aaactaacca aagaagacat cagtcaggaa atgtttgact tgtacgatga
     2941 ttatgctcac aataaaattg acagaaggca atttatcaaa gatttgtcca tttttgcaat
     3001 tggaggtctg tcattaccgt cgcttcttag ctttatgacg cctaattatg tggataccat
     3061 gactataaaa cctgaggatc cgagattaat ttcaaaattc attacttacg attctccaaa
     3121 aggtggtgga actatcaaag gtcttctttc taaaccctct aaaacaagga aaaaattacc
     3181 gggaattatc attgtacacg agaatcgagg actaaatcct tacattgaag atgtgggaag
     3241 aagagctgca ttagagggat ttattacgtt ggctcccgat gcattgtcac cattaggtgg
     3301 ttatccgggt aatgatgatg caggaagaga attacaaaaa aaacgcagca gcgaagagat
     3361 gcttgaagac tttattgctg cctacgagta tttaaaatca cataaaaact gtaatggtta
     3421 tgtcggagtg gttggatttt gttttggagg ttggatttcg aatatgatgg cagtaaaaat
     3481 acccgattta cctgcagcag taccttatta tggcgggcaa cccactgccg tagaagctga
     3541 aaaaatatca aaccccatca tgttgcactt tgccggttta gatacaagag taaatgaagg
     3601 ttggccggct tatgaggaga tacttaaaaa gaacaaagtg gaatataccg cctattttta
     3661 cgaaggtgta aatcatggtt ttcacaataa ta
//
LOCUS       DAORKP010000186         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7077_length_3670_cov_7.885754, whole genome shotgun sequence.
ACCESSION   DAORKP010000186 DAORKP010000000
VERSION     DAORKP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7077_length_3670_cov_7.885754"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLN75_08650"
     CDS             complement(<1..473)
                     /locus_tag="PLN75_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRG04224.1"
                     /translation="MKNNQFEKELVALQSNMRNFAFSLTLNRDDAEDLLQDTTLKVLD
                     NQDKFTENVNFKGWVLTIMKNIFINNYRKIVRNQTVFDRTEDLHHLNLPQNSGFETPE
                     GAYSVGEISNNINAFADEYRIPFSMHIQGFKYEEIAQKMNLPIGTVKSRIFLARK"
     gene            688..2091
                     /locus_tag="PLN75_08655"
     CDS             688..2091
                     /locus_tag="PLN75_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRG04225.1"
                     /translation="MNKILIILSIVISSLLASGNLYSNNQARALYFTIDLKKNVGSTT
                     WIYIQKGFEEARKANANTIVIEMNTYGGEVSYADSIRTKILNSDIPVIVFIDNNAASA
                     GALISIACDKIYMSQAATIGAASVVDQTGQKLPDKYQSYMRATMRATAEAHGKDTVVT
                     GNDTVVKWKRNPLIAEAMVDERTVVPNVADSGKILTFTTQEALKYGYCDGTAENIREI
                     IKKESGNQPYELVKFKPTAWDNIKGFLMSPFFQGILIMLIIGGIYFELQSPGIGFALG
                     VAITAAVLYFAPLYIDGLAANWEIILFVVGLILIGLEIFVVPGFGITGISGIFLVVSG
                     LILSLLNNVNFDFKPIHPADSSKAILTVIGGLTLGFGLIIYLSNKIGSKGLFKKLALE
                     TTLENKEGYIGVPTEGVNIVGKKGIAYTVLRPSGKVKVDEKVYDAVSVNGVFIEKGTQ
                     IIVVRYETGQVYVESAE"
     gene            2421..>3670
                     /locus_tag="PLN75_08660"
     CDS             2421..>3670
                     /locus_tag="PLN75_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRG04226.1"
                     /translation="MKKRLKLLCILFFIGIGVSFAQTRVTGSVTDENGEPIIGASVMV
                     KGTNVGISTDANGLFTLIVPGNASTLVFSYVGMNSLELPVRPQMDVLLRSNAELLEEV
                     VVVGYGTQRKANLTGAVSTVDVSKTLEARPYSEISKALQGVVPGLTVISNSGKLGAQP
                     TLTIRGIGTLSNSGTSKPFIVVDGVPMEDISLLNTQDIENISVLKDAASTSIYGTRAA
                     FGVILITTKSAKKTDRTTVNYTNNFGWDTPTILPNYPDVPTQLRALIAANGRAGLANE
                     LFGMYLDEMLPLAEKWKTDHNGQKTGYREMILGEDFVLDKNGVGSYFADWDVVNIMFR
                     KWKPTRSHNLSVQGTSGKTSYYMSLGNNHDEGVMTYYPEKMNKWTTMLNVTTQAFNWL
                     QVGGRFNYSNKGFQGPALQRRTYE"
BASE COUNT         1120 a          703 c          783 g         1064 t
ORIGIN      
        1 cttttacgtg cgaggaaaat acggcttttt acggttccga tgggcaaatt cattttctgt
       61 gcaatttctt cgtatttgaa gccttgtatg tgcatggaaa acggaatgcg gtattcatcg
      121 gcaaatgcat tgatgttgtt actgatttct ccaacagagt aagcaccttc aggggtttca
      181 aaaccggagt tttgcggcag attcaaatgg tgaaggtctt cggtacgatc aaatacagtc
      241 tgatttcgaa caatttttcg ataattgttg ataaaaatat ttttcataat ggtcagaacc
      301 caacccttga aatttacatt ctcagtaaat ttatcctgat tatcaagcac tttcagggta
      361 gtatcctgta gcaggtcttc ggcatcatcg cgattgagcg ttaaagaaaa tgcgaaatta
      421 cgcatgttac tttgtagggc aactaattcc ttttcaaatt gattgttttt catctcgttt
      481 ttagtttttg tgtgtgtaaa ttttagctaa aacgtatctt tttgaaaaaa tgtaacttcc
      541 ccggaaggaa gatacgcaaa gaatgtcagt tgtctatact gaatattttt taaaaaaaga
      601 attccgaaaa ttgttttgta tttttccact acaaatatag ttgattattt cttaaattga
      661 ctatatttgt aacaaaattt ttaacacatg aataagattt taattatttt aagcattgtt
      721 atctcaagtt tgcttgcaag tgggaattta tacagcaata atcaagccag ggctctgtat
      781 ttcaccattg atttgaaaaa aaatgtgggg agcactacct ggatttacat acaaaaaggc
      841 tttgaagagg ctcgtaaggc caatgcgaac accattgtca tcgaaatgaa cacctacggc
      901 ggcgaggtga gctatgccga ttcgatccgg acaaaaattc tgaatagtga cattcctgtc
      961 atagttttca ttgacaataa tgccgcttcg gccggagcac tcatatccat tgcctgcgat
     1021 aagatttaca tgagtcaggc tgccacaata ggtgctgcat ctgtagtgga tcagacgggg
     1081 caaaagctgc ccgacaaata tcagtcgtac atgcgggcta ccatgcgggc cacagccgaa
     1141 gcccacggca aagataccgt ggtgaccgga aacgatacgg tggtaaaatg gaaacgcaat
     1201 ccgctcattg ccgaagccat ggtggatgaa cgtaccgtag tgcccaatgt ggcagattcg
     1261 ggcaaaatac tcactttcac cactcaggaa gcgctaaaat acggatattg cgacggaact
     1321 gccgaaaata tccgggaaat aataaaaaag gaaagcggaa atcaacccta tgaactcgtc
     1381 aaattcaaac ccaccgcctg ggataatatt aaaggttttc tgatgagtcc tttttttcag
     1441 ggaattctga tcatgctgat tattggggga atttatttcg aacttcagtc gcccggcatc
     1501 ggatttgcgc tgggagtagc cattacagcc gctgtgcttt attttgcccc gctttacatc
     1561 gacgggcttg cggcaaactg ggagatcatt ctgtttgttg tcggacttat cctcatcggg
     1621 ctggagattt ttgtagttcc gggttttggg attacgggca taagcggtat cttcctggtg
     1681 gtttcagggc tgattttgag cctgctaaac aatgtgaatt tcgacttcaa accgattcac
     1741 cccgccgata gcagtaaagc cattctcacc gtcatcggtg gtctgaccct tggtttcggg
     1801 ctaattatct acctgagcaa taaaatcggc tccaaagggc ttttcaaaaa gctggccctg
     1861 gaaaccacgc ttgaaaacaa ggagggatac atcggtgttc cgactgaggg cgttaatatc
     1921 gttgggaaaa aaggcattgc atacaccgta cttcgcccct ccggcaaagt gaaagttgac
     1981 gaaaaggtgt acgatgctgt atctgtcaac ggagttttta tcgaaaaagg cactcaaatc
     2041 attgttgttc gctacgagac cggacaggtt tacgtagaaa gtgccgaata acaccacgaa
     2101 tacatacttc cgaagaaaat caccatgcat ggatttggtc aaaatgtccg gattcagtaa
     2161 ctttcttgtt tttatttttt cgctccggtt tatttcaaat ttactttttg ggacaaaatg
     2221 atgttcaaaa aaacactttt gcgcattttt cgtcaattat attaccaaat agatttttac
     2281 acattcttta actttgttaa acttaaaaag aaagaatcta tacatatttt tttggataat
     2341 tttgtgaaac cctttattca tcgaaaaaca aaaaagatat ttttccttaa cattttcaat
     2401 taacaaaaat taatttttac atgaaaaaac gattaaaact actgtgcatt ctattcttta
     2461 tcggaatagg tgtaagcttt gcacaaaccc gtgtcacggg cagtgtgacg gacgaaaacg
     2521 gagagccgat cattggagct tctgttatgg ttaaaggaac aaatgtgggt ataagcactg
     2581 atgctaatgg tttattcaca ttaattgttc cgggaaatgc atccacatta gtattttctt
     2641 atgttggtat gaattcgctt gaacttccgg tacgtcctca aatggatgta ttactccgat
     2701 caaatgcaga attgctggaa gaagtagtag tagtgggtta cggcactcag cgcaaagcca
     2761 atctgaccgg agctgtatca actgtggatg taagcaagac attggaagcc aggccttatt
     2821 ctgaaatatc aaaagctctg caaggagttg ttccaggctt gacggtaata agcaacagtg
     2881 gaaaactagg tgctcagcca acccttacga ttcgtggaat cggaacatta agtaacagcg
     2941 gaaccagtaa accgttcatt gtagtagatg gagttccgat ggaagatatt tccctgctca
     3001 acacccagga tattgagaat atttcagtac tgaaagatgc tgcatctact tctatttatg
     3061 gaacacgcgc agcatttggc gtgattttga ttactaccaa atcagctaaa aagaccgata
     3121 gaactactgt aaactatacg aataattttg gatgggatac acctactatt cttcccaact
     3181 atccggatgt acctacccag ttaagagcat taattgcagc taacggacgg gcaggattag
     3241 caaacgaact tttcggaatg tatctggatg aaatgcttcc tttggctgaa aaatggaaaa
     3301 ctgaccacaa cggacaaaaa acaggctatc gcgaaatgat attaggcgaa gactttgtac
     3361 tcgataaaaa tggtgttgga agttattttg cagattggga tgtggtgaat attatgttta
     3421 gaaaatggaa accaaccaga agtcacaatc tttctgtcca gggaactagc gggaaaacat
     3481 cctattacat gtccttgggc aataatcatg atgagggagt gatgacatat tatcctgaaa
     3541 aaatgaataa atggacaacc atgctaaatg taacaacaca agcatttaac tggttgcaag
     3601 tcggaggtcg cttcaattac agcaacaaag gattccaggg cccggcatta caacgcagaa
     3661 catatgaata
//
LOCUS       DAORKP010000187         3667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7092_length_3667_cov_7.372924, whole genome shotgun sequence.
ACCESSION   DAORKP010000187 DAORKP010000000
VERSION     DAORKP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_3667_cov_7.372924"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="PLN75_08665"
     CDS             <1..1817
                     /locus_tag="PLN75_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG04227.1"
                     /translation="RRFIPPYKKYVFLSLLSNLLSTVFSLFSFAAIIPILQILFGVTT
                     SKAVYQPWAWGDGLENVYKALKNNLSYWIETTIVQQGPTKALIYIGLFLIVMTFLKVG
                     TSYMGSYFVIPIRTGVLRDLRNKMYAKIITLPIGFFSDERKGDIMSRMTNDVVEVEAS
                     IISSLDMVLKNPLMILIYLVVMFVMSWKLTLFVLILLPVSVWIIGIIGRSLRKRSQLG
                     QEQTGEMLSQIDETLNGLRVVKAFNAENILTERFTRLNAKIRKTFHRIHRKYNLASPV
                     SELFGTIVIAILLWFGGYLILSANSPIGAAEFIYYLVVFYLIVPPAKELSKASYSIRK
                     GMASMERIDKILQEENNIPDPVNPQSLPGKIEKIEYRNVSFRYQTDWVLRNVDLVIPV
                     GKTVALVGQSGSGKSTLVDLLPRFYDPDEGEILFNEIDIRNFTKYDLRELMGNVNQEA
                     ILFNDTFFNNIAFGMEGATTEQVIQAARIANAHDFIMATENGYNTTIGDRGGKLSGGQ
                     RQRISIARAVLKNPPILILDEATSALDTESEYMVQEALDNLMKNRTTLVVAHRLSTIK
                     RADLICVIHEGKIVERGKHDELIALDRHYKRLVDMQSF"
     gene            complement(1814..3022)
                     /locus_tag="PLN75_08670"
     CDS             complement(1814..3022)
                     /locus_tag="PLN75_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04228.1"
                     /translation="MKSGIWRHIFMVFTILSYSLNAQVSVLMQPDRKATASGTISEIK
                     ETGSDNSETANSFSSEYHHVKTISESSAFPDLLMMDEGRTTYSDLNKVAANPSSNYFH
                     TESRTVFPFRMGEFERQQIIEFPGNPGKISVIYVAPKGKLSFTISLTPTNEGTEGRLR
                     NEYLKEIKKLSGEEKSDTLPTPDPIKYVGRNYICNGVEGCFTNRQAGSISQLTVFECG
                     VWLLCIRLKTNDTDAARNALLYESLTRRFNPAALTALSPLNLKSNVGFIGDSMKDAET
                     SSAMVNSAFKKMDWATRNVSSKERYSGFPDIYLAMHLESAKEYLRIQGKKMITGNPKT
                     DHFYEDLNAISNAGFLPEFIMEEYDRVMIVPDDLKLNFEAYREWKKGRQISASLKEKK
                     YIITYRNLPY"
BASE COUNT         1010 a          776 c          815 g         1066 t
ORIGIN      
        1 tcaggcgatt cattccgcct tacaagaaat atgtttttct gagcttgctg tccaatctcc
       61 tttccacagt gttcagcctc ttttcttttg cggcaattat tcccattctt cagatactgt
      121 tcggggtgac cacctccaaa gccgtttatc agccctgggc ctggggcgac ggactcgaaa
      181 atgtgtacaa agcgctcaaa aacaatttat catactggat tgagactacc atcgttcagc
      241 agggaccaac caaagccctg atttacatcg gattatttct tattgttatg acgttcctga
      301 aagtgggaac ctcgtatatg ggatcttatt tcgtaatccc tatccgaacg ggtgtgctgc
      361 gcgatttgcg aaataaaatg tacgccaaaa taattacgct gcctattgga tttttctccg
      421 acgaacggaa aggtgatatc atgtcacgta tgaccaacga tgtggtagag gtggaagcat
      481 ccatcatcag ttcgctggat atggtgctga aaaatccgct gatgatattg atttacctcg
      541 ttgtgatgtt tgtaatgagt tggaaactca cgctgtttgt actgattttg ctaccggtga
      601 gcgtgtggat aataggtatc atcggcaggt cgctgcgtaa gcgctcgcag ctggggcagg
      661 agcaaaccgg tgaaatgctc tctcagattg atgaaacact caacggactg cgtgtggtga
      721 aagctttcaa tgccgagaat attctgaccg aacgattcac ccgcctgaat gccaaaatca
      781 ggaaaacatt tcaccggatt caccgcaaat acaacctggc ctctccggtt agcgaactct
      841 ttggaaccat tgtaattgct attttgctat ggtttggcgg atatcttatc ctgagtgcaa
      901 acagtccgat cggagcagcc gaatttatct attatctcgt tgttttctat ctgattgttc
      961 cgcctgccaa agaactttct aaagcctcgt acagtatacg caagggaatg gcttcgatgg
     1021 aacggattga taaaatactg caggaggaga acaatatccc cgatcccgta aatccgcaat
     1081 ctctacccgg gaagattgaa aaaattgaat accggaatgt cagtttcagg tatcagaccg
     1141 attgggtact tcgtaacgtg gatttggtta tccctgtcgg aaaaacggtt gcacttgtcg
     1201 gtcagtcggg ttcgggaaaa tccacactgg tagatttact gccccgattt tacgatccgg
     1261 atgaaggtga gattttgttt aatgaaatcg atatccgaaa ttttacaaaa tacgatctga
     1321 gggaactcat gggtaatgta aatcaggaag ccattctttt taacgataca tttttcaata
     1381 atatagcatt cggtatggaa ggagcaacaa ccgaacaggt gatacaggct gccagaatag
     1441 ccaatgcgca cgatttcatc atggctaccg agaatggcta caataccact atcggcgaca
     1501 ggggaggcaa actctccgga ggtcagcgtc agcggataag cattgccaga gctgtgctga
     1561 aaaatccgcc catcctcatc ctggatgaag ctacctcggc gctcgatacc gagtcggaat
     1621 acatggtgca ggaagcactg gataacctga tgaaaaaccg gacaacactg gtagtggctc
     1681 atcgcctctc aaccatcaaa cgcgccgatc tgatttgcgt catccatgaa gggaaaatag
     1741 tggagcgcgg caaacacgat gagctgattg cactcgaccg tcattacaaa cggctggtag
     1801 atatgcagtc gttttagtac ggaagattcc ggtaggtgat aatgtatttt ttttctttca
     1861 ggctggcgct tatctgccgg ccttttttcc actcacggta agcttcgaaa ttcagtttca
     1921 aatcgtccgg tactatcatc acccggtcgt attcctccat gatgaattcg ggtaaaaatc
     1981 ccgcattgct gatggcattc agatcttcgt aaaaatggtc ggttttcgga tttccggtaa
     2041 tcattttctt cccctggatt ctcaggtatt cttttgccga ttccaggtgc atagcaagat
     2101 aaatatccgg aaaacctgag taacgttctt tactgcttac gttgcgggta gcccagtcca
     2161 tttttttgaa ggcagagttc accattgctg agctggtctc ggcatctttc atcgaatcac
     2221 cgataaaacc cacatttgac ttcagattca gcggactcaa ggctgttaaa gccgccggat
     2281 tgaaacgccg ggttaaactt tcgtagagga gggcattccg ggcagcatcc gtgtcgttgg
     2341 ttttcagcct gatgcaaagc agccacacac cacattcaaa caccgttagc tgtgagatag
     2401 aaccggcctg ccggttggtg aagcagccct ctacaccgtt gcaaatgtaa ttacgtccca
     2461 cgtactttat tggatcaggt gttggaagcg tgtcggattt ttcttctccg gatagttttt
     2521 tgatttcttt caggtactcg tttctcaatc gtccttcggt gccctcattg gttggcgtaa
     2581 gactgattgt aaaggataat ttgcctttcg gagcaacgta gattactgaa attttgcccg
     2641 ggtttccggg gaattcaatg atttgctgac gctcaaattc gcccattctg aaaggaaaaa
     2701 cagtacgact ttcggtgtga aaataattgg atgaaggatt tgcagccact ttattcaggt
     2761 cagaatatgt tgtccggcct tcgtccatca tcagtaaatc cggaaatgcg ctgctttcgg
     2821 agattgtttt tacgtggtgg tattcagatg aaaacgaatt ggcagtttca gaattatcag
     2881 atccggtttc tttgatctcc gatatggttc ctgaagcagt agcttttctg tcaggttgca
     2941 tcagtacgct cacctgagca ttcagtgagt acgacagaat agtaaaaacc atgaatatat
     3001 gtctccagat tccggatttc attttttatt aaaaattaag tgcaacatgc aagttcaacg
     3061 gtttacccgt tttttttata ctttgaagtc ttgatttttt tgtatctttt ttcaccaata
     3121 atcataccag tgatatattt ctgccaaaaa aaacaaatag agttgttttt ttttgttcta
     3181 gattgtatat ctgacggaac accacaatga atgaggcgtt tgatgaaata caattatgag
     3241 aaatagtaca tcttgcagaa tatcaattgc tgtttcagtt ttcaagaagc ttgccgatgt
     3301 gaaaaacctg atatttcaat gaattaaccg aaaaaaaaag gaaaaacgcc tgcaaaaata
     3361 aaactaaaaa acgagttgga caaacacgaa atgcccggca aggctgaagt taattaaaag
     3421 ttcatttacg cttatgtatg caatggaaat atttctgaaa taaattgtgt atttttgttc
     3481 ccttgcagtt tgtgtgaaac tgacaaatta atacccggtc aacaactaaa aaaacaagga
     3541 atatgaaaaa ggtaatattt ttaatcgtgc tgtcaatcag cgtgttgaat gcatacacac
     3601 agcagttgaa ttcccccgac gggaattttc agttgaattt ctcactgagt aatgccggtg
     3661 agcctgt
//
LOCUS       DAORKP010000188         3650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7143_length_3650_cov_10.229764, whole genome shotgun sequence.
ACCESSION   DAORKP010000188 DAORKP010000000
VERSION     DAORKP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_3650_cov_10.229764"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..>3650
                     /locus_tag="PLN75_08675"
     CDS             369..>3650
                     /locus_tag="PLN75_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRG04229.1"
                     /translation="MKSLSLQLISIFWVLCITVNAQTGIFYSTDKDLSNSLINSIYQD
                     KRDYIWIATEDGLNKFDGVKFSVYKNNLKDSTSLKNNYVRCMYEDSKGRFWVGGINGL
                     QLFNRTSNKFSNIHLYSSNNILQPHVTSIFESKDGTIWISTSGEGVLKLNPNNNSRFE
                     ADARLNSKLPSIHLMFVYQDSYNQLWIGTENHGLCMYDTGSGAIRSFDVKTGNIDNNR
                     ISAIAEDDKGNIFIGTLAGGIFKLNREKWDFESIPRANSPVPLPVKSLFIDNQKQLLI
                     GTDGLGLKYYNYENNRVEDYQMSSSIFDFSNVKVHSILQDRRGNLWIGLFQKGVFLLR
                     NNPYRFNYIGNKYHNQDLIGSSCVTAVYKDKDYLWIGTDNDGMYQLDKQYRVRHFAHT
                     NTPGSITSTVMSIVQTKNGEIWLGSYLNGLALFNKSTGSCTYFNDKPANLGNSTFDNK
                     INCMIADENNVLWIGTHGGGVYTFDTNTRKYVKHYSFPYITNDWINCLFKDKNGMVWA
                     GTHKGFSIIDTKKNQVKNFSENNEITPGKVIYSIIQDKKDNIWIGTTEGVAIYDVKNN
                     KSQKLTVENGLASDVICGLVEDAGGNIWMSTHNGISKYNPNDSTFSNYYSSDGLQGNE
                     FTKGAFFKSENGEVFFGGISGLTSFFPEQIKEDRESLKLFVTALNIGDRVVFNDGRKG
                     KAGNRFISDLDTIFLDYKDNIFNLEFSTFNYGVAEQVHYRYKMEGLNDTWISTNPGVN
                     RIHFTNINFGTYKLKVTASLRDLESEVREITIIISPPWYLTNLAIATYIILAMLLLYG
                     VARFILDRIKHRQEMMMRQHAEQLNEAKLQYFINISHEIRTPMSLIISPLEKLIPGEK
                     DPQKIYLYQIMHRNAKRILRLINQLMDIRKIDKGQLVLKFRETDIVGFIQDLMQTFEY
                     TANKRNMDFQFVHDQPALKVWIDLNNMDKVLMNILSNAFKFTPDNGKITIKLNTGTAP
                     EAEGALKNYFEITVSDSGIGINESDLENIFARFYQIDNDYTKSQIGTGVGLHLSRSLV
                     ELSHGIIFARANSEGPGSEFVIRLPLGNSHLSRHEMIGEMAPTETSAITHNVEKENLE
                     VLSEVI"
BASE COUNT         1182 a          741 c          713 g         1014 t
ORIGIN      
        1 gatgtttttc attattttct cattaaactt aataaaatgt tttctggata acgttaacat
       61 tttctctgta taatattaat gtatcatatg gatacaaatt atatggaaat gttaaaaata
      121 actggttaga ttgggttaat aaaggtcaac ttccgtcttt ttttctcatg attataatta
      181 ttaagagtta acaatattta ttaattgcag tgttacaaat ttattaaata tactagtcgg
      241 ggctttacct aattattaca gatacttttc taattgttac atttaaagtt aaaatgtttc
      301 attttttagc agttttgata cattcattat ttttttacta catttgtaac caaatattac
      361 ctgaaatcat gaaatcactt tccctccaat taatatccat tttctgggtg ctttgtatca
      421 ctgtgaatgc ccaaaccggc atattctatt ccacggacaa agacctgtct aacagcctga
      481 taaacagcat ttatcaggat aaacgcgact atatctggat tgctactgaa gatgggttga
      541 ataagtttga cggagtaaaa ttttctgttt acaaaaacaa cctgaaggac tctacatcgc
      601 tgaaaaacaa ctatgtacga tgtatgtacg aagattcaaa aggtagattt tgggttggag
      661 gcatcaatgg attgcagctt ttcaaccgga cttccaataa attctcaaat atccacctgt
      721 attcctccaa taatattctc caaccgcatg ttacatctat atttgaaagc aaagatggaa
      781 caatttggat aagtacttcc ggagaaggag tcctgaaact caacccgaat aataattccc
      841 gctttgaggc agacgcccga ctgaattcaa aactgccaag catccacctg atgttcgtgt
      901 atcaagacag ctacaaccag ctttggatcg gaaccgagaa tcacggactt tgcatgtacg
      961 acaccggaag cggcgctatc cgatcttttg acgtaaaaac cggaaatatt gacaataacc
     1021 gtatttcagc catcgctgaa gacgataaag gaaacatttt tatcggtacg cttgccggag
     1081 gaattttcaa actcaacaga gaaaaatggg attttgaatc gataccacgt gctaatagtc
     1141 ctgtgcccct tcctgtcaaa agtcttttta tcgataacca gaaacagctg cttatcggaa
     1201 ccgatggttt gggattaaaa tattacaact acgagaacaa ccgggttgag gactaccaga
     1261 tgtcttcatc gatttttgac ttctcaaatg ttaaagttca ctccattctg caggacagaa
     1321 gaggtaacct gtggatcggc cttttccaga aaggagtttt cctcctgaga aacaatcctt
     1381 accgttttaa ttacattggc aacaagtatc acaaccagga tttgattgga tcgagctgtg
     1441 tcactgcagt ttataaagac aaagattatc tctggatcgg gactgacaac gatggaatgt
     1501 atcagcttga caagcagtac cgcgtgcgcc attttgctca cactaatacg ccaggctcca
     1561 ttaccagcac ggttatgtca attgtacaaa ccaaaaacgg ggaaatctgg ctcggctcct
     1621 atttgaacgg attggcactt ttcaacaaat ctacaggcag ctgcacctac ttcaacgaca
     1681 agcctgcaaa cctgggcaac agtacttttg ataataaaat caactgcatg attgcagatg
     1741 aaaataatgt gctttggatc ggaactcacg gtggtggagt ttacacattt gatacaaata
     1801 cacggaaata tgtgaagcat tattcgtttc catacatcac taacgattgg ataaactgcc
     1861 ttttcaaaga taaaaacgga atggtatggg cgggaacaca caaaggtttt tcaatcattg
     1921 atacaaaaaa aaatcaagtc aaaaatttct ccgaaaacaa tgaaatcaca cccggcaagg
     1981 tgatttactc cattattcag gataaaaaag acaatatttg gattgggact accgaaggtg
     2041 tggcaatcta cgatgtaaaa aacaataaat ctcagaaatt aacggttgag aatgggctgg
     2101 cgagcgacgt tatctgcgga ctggtagagg atgcaggcgg aaatatctgg atgagtacgc
     2161 acaatggcat ttcgaaatac aatccgaatg acagcacttt ttcgaattat tacagctctg
     2221 acggcctgca aggcaatgaa tttaccaaag gagcattttt caaaagtgaa aacggcgaag
     2281 ttttcttcgg tggtatcagc ggactaactt cattttttcc agaacagata aaggaagaca
     2341 gagagtcgct taaacttttc gtaacagccc tgaatatcgg cgacagagta gttttcaacg
     2401 atgggagaaa agggaaagcc ggtaatcgct ttatctctga ccttgacact atttttctgg
     2461 attacaaaga caacatcttc aatcttgagt tctcaacttt caactacggt gtggccgaac
     2521 aggtgcacta ccgctacaaa atggaagggc tcaatgatac ctggatcagc accaatcccg
     2581 gcgtaaaccg gattcacttc accaatatca atttcggcac ctataaactg aaagtaactg
     2641 catcactccg cgatctggag tctgaagtgc gtgaaattac cataattatt tctccaccgt
     2701 ggtatttgac aaatctggca atcgccacgt acatcatact tgcaatgtta ttactgtacg
     2761 gtgtagcccg atttatcctc gacagaatca aacaccgcca ggaaatgatg atgcgtcagc
     2821 atgccgagca actcaacgaa gccaaactgc agtacttcat caacatttct cacgagatca
     2881 ggacaccgat gagtctgatt ataagtcctc tggagaaatt aattccaggc gagaaagatc
     2941 cgcagaaaat ctatctttat cagatcatgc atcggaatgc aaaacgtatt ctcaggctga
     3001 ttaaccagct gatggacata cggaaaattg ataaaggaca gcttgtcctg aagtttcgtg
     3061 aaacggatat cgtcggcttt atccaggatt tgatgcagac ctttgaatat acggcgaata
     3121 aacgaaatat ggacttccaa ttcgttcatg atcagccggc tctcaaggtt tggatcgact
     3181 tgaacaacat ggacaaagta ttgatgaata ttctgtcaaa tgcattcaaa tttacgcctg
     3241 acaatggaaa aatcacgata aaactgaata ccgggacagc tccagaagca gaaggtgcat
     3301 tgaaaaatta ctttgaaatc accgtttcag actccggaat agggattaat gaaagtgatt
     3361 tggagaatat cttcgcccgt ttctaccaaa tcgataatga ttacaccaag tctcagatcg
     3421 gaacaggagt aggacttcac ttgtcccgat cactcgtgga actttctcac ggaatcatct
     3481 ttgcgcgagc taattcagaa ggacctggta gcgaatttgt tatccgcctg ccactcggaa
     3541 acagccattt gagcagacac gaaatgattg gagaaatggc acctacagaa acttcagcaa
     3601 tcacccataa tgttgaaaaa gagaatcttg aagttctctc tgaagtaatt
//
LOCUS       DAORKP010000189         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7282_length_3609_cov_10.016320, whole genome shotgun sequence.
ACCESSION   DAORKP010000189 DAORKP010000000
VERSION     DAORKP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_3609_cov_10.016320"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>2452
                     /locus_tag="PLN75_08680"
     CDS             81..>2452
                     /locus_tag="PLN75_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRG04230.1"
                     /translation="MKHRFFYLVLIITFSFNAFAQNGSIKGRVFNEKTNEPLEFATVQ
                     IQGTTIGSTTDLDGNFIFTGITPGFVRLLVTYVGFEKTLSPEIQVQGNQTAFIDIPVP
                     EASFVFKEVVVRSNLNIKRIESPVSVLSVGVQQIEKTAGANRDVSKVVQTLPGVGATD
                     PNRNDLIVRGGGPSENVFYLDGVEIPIINHFATQGSSGGAVGIINPDFIREIDFYTGA
                     FPANRPNALSSVMELKQKDGSSDRIHSKLSVGASDAAFTLDGPIGDKSTFIVSARQSY
                     LQLLFKVIGLPFLPTYNDFQLKYKYKIDQKNEITFIGLGAIDNMTLNTDLQDTGTEAQ
                     KYLLNYLPIYKQWNYVVGTVYKHFGDNHYDTWVLSRNMLRNNNFKYRNNDENLPKISD
                     YTSDEAENKIRFERMYPDLPVKLLFGGGLKLAHYVNETNRKIFKDGQLSDLQYSTDLN
                     LLGYQAFVQASDSYLDERLRLSLGLSTIGNNYNKNMSNPLNQLSPRLSLSYKLNDKLD
                     FNSNFGRYAMQPAYTTLGYKYTTGGDYVNKNEKLKSVISNQAIVGFDYRPAEKLRFTL
                     EGFYKQYMNYPLSVADGLSIASKGTDYGQVGDEEIISTGKGRAYGVEFLAKIIEWKQL
                     NTSLTYTFFRSEFTDKNEVYRPSSWDTRHVLNLISSYKFAGSWNIATRWRFVGGAPYS
                     PIDMALSENKDAWSVTNQAIVDYRNFNSLRLKNSHQLDIRIDKEFYFKKWVLNLYTDI
                     QNAYNFKSQSPPIYTNRDAEGNILKHPSNREDLQGLRMIETYNGTILPTIG"
     assembly_gap    2453..2552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2553..3092
                     /locus_tag="PLN75_08685"
     CDS             <2553..3092
                     /locus_tag="PLN75_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04231.1"
                     /translation="TGKYIQTLYVSESIGKGTFKRADRNTGKWMPGEIQRPATLPYWF
                     HERNITNERGTLLPTPERPVVDAYTGATPLSSFEMTLMTDEKLNGKYQLFLELNQSWD
                     WNEYWTNNKYPDNKEYKTSSQPAVVYMAAFDTNDPKTIELKPVGHSHYAGESGELFTD
                     LSTLSTALKIAKKITISVK"
     gene            3254..>3609
                     /locus_tag="PLN75_08690"
     CDS             3254..>3609
                     /locus_tag="PLN75_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRG04232.1"
                     /translation="MKILILGAGKMGTFLTDVLCIQHEVALYDTDARKLRFVFNTLRL
                     TQLDEIREFEPELLINCVTLKYTIEAFEKVLPYIPQNCIISDIASVKTGLQEYYENCG
                     RRYVSTHPMFGPTFATL"
BASE COUNT         1117 a          780 c          729 g          883 t
ORIGIN      
        1 aatcgaacta aatataaaac aaatatgagc tataaatgtt taatttagaa cgaaaacatt
       61 ctaaaataat ttatataaaa atgaaacatc gattttttta tttagtatta ataataacat
      121 tttctttcaa tgcatttgcg cagaacggga gcataaaagg gcgtgtattc aatgaaaaga
      181 ccaatgagcc acttgagttt gctaccgttc agattcaggg aacaaccatt ggttcgacta
      241 ccgacctcga tggaaatttt attttcaccg gaatcacacc gggattcgtc agattattag
      301 ttacttatgt aggttttgag aaaactctat ctccggaaat tcaggttcag ggtaatcaga
      361 cagcatttat agatattccg gtacccgaag catctttcgt tttcaaagaa gttgtcgtga
      421 gaagcaatct gaatatcaag cgcatcgaga gtcctgtttc ggttttatcc gttggagtgc
      481 agcagattga aaaaactgcg ggtgccaacc gcgacgtgtc aaaagtagta cagacactgc
      541 ccggagtggg tgctacggat ccgaaccgca acgacctgat tgtacgcgga ggcggaccct
      601 ctgaaaatgt gttctatctg gacggtgttg agatacctat tatcaaccac tttgccacac
      661 agggttcttc gggcggagcg gtaggcatta tcaatcccga ttttatccgc gaaattgatt
      721 tttacaccgg tgcttttccg gccaacaggc ccaatgcgct gagttctgtc atggaactga
      781 agcaaaaaga cggaagttcg gaccggatac attcaaaact gtcagtcgga gcatcggatg
      841 cagctttcac actcgacgga ccaatcggcg ataaatccac attcattgta tcggcacgcc
      901 aatcgtacct ccagctcctt ttcaaggtca tcgggctgcc gtttctaccc acttacaacg
      961 atttccagtt gaagtacaaa tataaaatcg accagaaaaa tgaaataaca tttattggac
     1021 tcggtgccat tgacaatatg acactcaata ccgatctgca ggatacagga actgaggctc
     1081 agaagtatct gctaaactac ctgccgatat acaaacagtg gaattacgtg gttggaaccg
     1141 tttataagca ctttggcgac aatcattacg acacgtgggt actgagccgc aacatgctca
     1201 gaaacaacaa cttcaaatac agaaataacg acgaaaacct gccaaaaatc tccgattaca
     1261 catcggacga agccgaaaac aaaatccgct tcgaacgaat gtatccggat ttacccgtaa
     1321 aacttctgtt cgggggtggg ctgaaactgg cgcattacgt caacgaaaca aacagaaaaa
     1381 ttttcaaaga cggacagcta agcgatttgc aatacagtac cgatttgaat ttactgggat
     1441 atcaggcctt cgtacaagcc tccgattcgt atctggatga aaggttaagg ttatcacttg
     1501 gtttaagtac cattggcaac aattacaata aaaacatgag caatccgctc aatcagttat
     1561 ctccaaggct ttctctttct tacaaactga acgacaagct ggattttaac tctaattttg
     1621 gcagatatgc catgcagcct gcttatacta ctttaggtta taaatatacc accggaggtg
     1681 attatgtaaa caaaaatgaa aagctcaaat cggtgatatc caaccaggcc attgttggtt
     1741 ttgactatcg tccggcagaa aaactgaggt ttacactgga aggattctac aagcagtata
     1801 tgaattatcc gctttccgtt gctgatggat taagcatagc cagcaaaggg actgactacg
     1861 gccaggtggg cgacgaggaa atcatctcaa caggtaaagg aagagcttac ggagtagaat
     1921 ttctggctaa aatcatagaa tggaaacagc ttaacacctc gctgacctat acatttttca
     1981 gaagtgaatt caccgacaaa aacgaagttt accgcccatc aagctgggac acgagacacg
     2041 tgctcaacct gataagcagt tacaaattcg ccggaagctg gaacattgca acacggtggc
     2101 gcttcgtagg cggagcacct tattcaccaa tcgatatggc gctgtctgaa aacaaagacg
     2161 cctggagcgt aaccaatcag gcaatcgttg attacagaaa tttcaactca cttcgcctta
     2221 aaaattcaca tcagctggat ataaggattg acaaagagtt ctatttcaaa aaatgggtgt
     2281 tgaatttgta caccgatatt cagaatgcct ataatttcaa gtcacaaagt ccgccgatat
     2341 ataccaaccg ggatgccgaa ggaaatatac tgaaacaccc aagcaaccgc gaagacctgc
     2401 agggattacg tatgattgaa acgtataacg gcaccattct gcccactatc ggnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccggaaa gtacattcag acactttatg
     2581 tatcggaaag tatcgggaaa gggactttca aaagagcaga ccggaacacc ggaaaatgga
     2641 tgccgggtga aattcagcgt cccgcaacac tgccttactg gtttcacgaa cgcaatataa
     2701 caaacgaacg cggcaccctg ctccccacac ccgaacggcc tgtagtggat gcttataccg
     2761 gagccacacc actttcgtcg ttcgaaatga cactgatgac cgacgaaaaa ctgaacggaa
     2821 agtatcaact gtttcttgaa ctgaatcaat cttgggactg gaacgaatac tggaccaaca
     2881 ataaataccc cgacaacaag gaatataaaa catccagcca gccggcagtg gtttacatgg
     2941 ccgctttcga caccaacgac ccgaaaacca tagaactgaa acctgtcggt cacagccatt
     3001 acgccggcga gagcggtgaa ttgtttaccg atttgagtac gttgtccaca gcattgaaaa
     3061 tagcaaagaa aattacgatc tcggtaaaat aaacttacag aaagaacatt cgtctgaatt
     3121 gcataaaaaa agctgtattt cctgacaagg tctttgattg acggaaatac agcttttata
     3181 attttttacc gctattatcc cgtcctttca atcgattttt cataactttg caccttaaaa
     3241 ttaagcgaat aaaatgaaaa tactgatatt aggcgccgga aaaatgggca cttttctcac
     3301 cgatgtgctc tgtattcagc acgaagtggc tctctacgat accgacgcgc gtaaactgcg
     3361 atttgttttc aacacgctgc ggcttactca gttggatgaa atcagggaat ttgagccaga
     3421 gttgttaatt aattgtgtta cacttaaata taccatcgaa gcattcgaaa aggtgttgcc
     3481 ttatattcct caaaactgca tcatttcaga cattgcttcc gtgaaaaccg ggctgcagga
     3541 atattatgaa aattgcggca gacgttatgt ttccacacac cctatgtttg gccccacatt
     3601 tgccacact
//
LOCUS       DAORKP010000190         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7304_length_3602_cov_9.126586, whole genome shotgun sequence.
ACCESSION   DAORKP010000190 DAORKP010000000
VERSION     DAORKP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7304_length_3602_cov_9.126586"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="PLN75_08695"
     CDS             <1..1521
                     /locus_tag="PLN75_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HRG04233.1"
                     /translation="RKGFATVTAEYRLIPEALYPAGVEDLNDAVKWIFDNAEKYNFDK
                     LKIAVSGCSAGGQLANLIGTKNENGLIKAIVNMDGLSAFIDEATIERAQKARDNGTKL
                     PVDAQWLGGTYSEKSQTWHEASALSWVNEKSAPVCFINSSIPRFHNGRDEQIQLLELS
                     NIYSEVHTFEKSPHTFWHFHPWHISTVNYMANFLNKIFQKNASEFDRKGFDIVVAQDG
                     SGDFISVQQAINAVPDFRKKQTRIFIRNGYYREKIIIPETKDSLILVGEDKFKTILSF
                     NNFASKPTGFGDQLGTSGSASVYISPANFSAENITFENGAGPVGQAVAVIVRSEKAQF
                     YNCRFLGFQDTLYTHKIGSRQYYKNCYIEGTVDFIFGSSVAFFEECEIFCKNNGYVTA
                     PSTPQNSPFGYVFYQCKINGENPDSFYLGRPWRPYGNVAFIECDMSDVIKSEGWNNWG
                     KASNEKTARFLEYKNTGKGGNLQNKRIKWAKTLTEKQAKKYTKENVLGKDFIFNGL"
     gene            1569..2804
                     /locus_tag="PLN75_08700"
     CDS             1569..2804
                     /locus_tag="PLN75_08700"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRG04234.1"
                     /translation="MLKFSRIFVSSQLLFFFVQVNSQTFTVNNCSNHEMKDIVVEIPV
                     RNLPISIGNYAVASKDGSTVPLEIVSNLKGNQLAVFPVKCLKPRSSNVFLLLKGNTDE
                     YPKRTYAELAHKIGGQFNGNTYEGGFSWVKPNYMKLPGTFRDHAYYIKYEGPGWESDK
                     VGYRFYLDQRNAIDVFGKKTPGIVLPGTGIDGYDSYHNMSDWGMDNMKVGKALGIGSI
                     AFWDGQKAVRVENRDSAVCFIPTDGKIRSQVNTTYYGWDVGGNKFNLKSLISIDAGSR
                     ASHMELQADKYVSNLTTGIIKNKDTELLKSDYNTGDWAYIATFGKQSLNNDNMGLAVF
                     YKKKQLKMLTEDDLNHLVVLTPENGYVEYYFMATWELEWQPVNNITDFMNALDEELIR
                     LNGLSYLKVSEKKDFKSLN"
     gene            2938..>3602
                     /locus_tag="PLN75_08705"
     CDS             2938..>3602
                     /locus_tag="PLN75_08705"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRG04235.1"
                     /translation="MKSFIHDDFLLQSDSARELYHNHSERQPIIDYHCHLNPEYIAKD
                     HKFDNLGQIWLEGDHYKWRAMRTNGIDEKYCTGKKTSDWEKYEKWVETVPYTMRNPLY
                     HWTHLELKRAFGVDELLNPASGKRIYDICTAQLRTPEFSARGLMKKFNVQVVCTTDDP
                     VDTLEHHISLKNEGFEIKVLPTWRPDKAMAVENPAEYRKYVEKLSEVSGVSISNFADL
                     LQA"
BASE COUNT         1143 a          725 c          771 g          963 t
ORIGIN      
        1 cgaaaaggtt ttgcaactgt caccgccgaa tatcgcctca tacccgaagc gctttatccg
       61 gccggtgtgg aagatttgaa tgacgcagta aaatggattt tcgataatgc tgaaaaatat
      121 aactttgaca aattgaaaat tgctgtttca ggatgttctg ccggtggtca gttggctaat
      181 ttgattggaa cgaaaaatga aaacggacta ataaaagcga tagttaacat ggatggcctg
      241 tcagctttta tcgacgaggc aaccatcgaa cgggcacaaa aagcgcgcga taacggcaca
      301 aaactgcctg tggatgccca gtggctgggt ggaacctatt ctgaaaaatc gcaaacctgg
      361 cacgaagcat cggctttgtc gtgggtgaat gaaaaatcag ctccggtttg ctttatcaac
      421 agctccatcc cgcgttttca taatggcagg gatgagcaaa tccaactgct tgagttgtcg
      481 aatatctatt ccgaagttca tacttttgaa aaaagtccgc acacattctg gcattttcat
      541 ccatggcata tttccacggt taattatatg gccaattttt tgaacaaaat tttccaaaaa
      601 aacgcaagtg agttcgatag aaaaggcttt gatatcgtgg tagcacagga cggcagtggc
      661 gattttattt cggttcagca agccatcaac gcggttcctg attttcggaa aaagcaaaca
      721 cgaattttca tccgcaacgg atactaccgc gaaaaaatca tcattcctga aacgaaagac
      781 agtctgattc ttgtcggcga agacaaattc aaaaccattc tttcattcaa taatttcgca
      841 tcgaaaccga ccggatttgg tgatcaactc ggaacatcgg gttcggcaag tgtatatatt
      901 tcaccggcta atttttcagc cgaaaacatc acatttgaaa atggagccgg accggtaggg
      961 caggcagttg cggtaattgt gagaagcgaa aaagctcagt tttacaattg ccgttttctg
     1021 ggatttcagg atacgcttta cacccataaa atcggcagtc ggcagtacta caaaaactgc
     1081 tacatcgaag gtacggtcga ttttattttc ggttcttcgg tggctttttt cgaagaatgt
     1141 gagattttct gcaaaaataa cggctatgta actgcaccat ccaccccaca aaacagtccg
     1201 ttcggctatg ttttctacca atgcaaaatt aatggtgaaa atccggattc gttctacctc
     1261 ggcagaccgt ggcggcctta tggaaatgtg gcttttattg agtgcgatat gagtgacgtc
     1321 atcaaatccg aaggctggaa caactgggga aaagcttcaa atgaaaaaac ggctcgtttt
     1381 cttgaatata aaaatacagg aaagggcgga aatttacaaa acaaacgtat aaaatgggct
     1441 aaaactttaa ccgaaaaaca agcaaaaaaa tatacaaaag aaaatgtgtt ggggaaagat
     1501 tttattttca acggtttgta aacacagaat tttttatttc tcattcaaaa aacaataaat
     1561 atcataatat gcttaaattc agtcgaatat ttgtttcttc tcagctctta ttttttttcg
     1621 ttcaggtaaa ttctcaaacc tttacggtga acaactgttc aaaccatgaa atgaaagata
     1681 ttgtcgtgga aattccggtg agaaacctcc cgatttcaat cggaaactac gctgttgctt
     1741 caaaagacgg atcgaccgtg ccgctcgaaa tagtttcgaa tttaaaagga aatcaactgg
     1801 cagttttccc tgtcaaatgc ctgaaacctc gttcttccaa cgtttttcta ctacttaaag
     1861 ggaataccga tgaatatcca aaacgcactt atgccgaact ggctcacaaa attggcggac
     1921 agttcaatgg aaatacttac gaaggcggat tctcatgggt taaacccaat tatatgaaac
     1981 tccccggcac tttccgtgat cacgcatatt acatcaaata cgaaggtccg ggctgggaga
     2041 gcgataaagt gggttatcgt ttttacctcg atcagcgaaa tgcaatagat gttttcggca
     2101 aaaagacgcc cggaattgtg ctgccgggaa caggtattga tggatacgac agctatcaca
     2161 atatgtctga ctggggtatg gataatatga aagtgggcaa agcgcttgga attggctcaa
     2221 ttgctttctg ggacggacaa aaggctgttc gggtggaaaa ccgcgacagt gctgtgtgtt
     2281 ttattccaac cgatggaaaa atacgctcgc aggtgaatac aacctattac ggctgggatg
     2341 ttggcggaaa taagttcaac ctgaaatcgc tgatttcgat tgacgccggc agcagagctt
     2401 cacacatgga attacaggcc gataaatacg tgagcaatct gactaccgga attatcaaaa
     2461 acaaagacac cgaactgctt aaatcagatt acaataccgg cgattgggct tatattgcca
     2521 ctttcggcaa gcaaagcctg aacaacgata acatgggact tgccgttttt tataaaaaga
     2581 aacagcttaa aatgcttact gaagatgatc tgaaccattt agtggtgctc acacccgaaa
     2641 acggatatgt tgaatactac tttatggcta cctgggaact cgaatggcag cccgtaaata
     2701 atatcaccga ttttatgaat gccctggatg aagaactgat ccgactcaac ggactctcat
     2761 atttgaaagt atccgaaaaa aaagatttta aatcccttaa ttaaacaaac tgatattttt
     2821 attataatat ttgataatag tgacaaatat tttttctcag tttcatttta tttgactaat
     2881 tttgtgcacg tacacaaaac cagcaaactg aagtaaagaa cttaaaacaa acgtaccatg
     2941 aaaagtttca ttcacgacga ttttttactg caaagtgatt cagccagaga actttatcac
     3001 aatcattcag agagacagcc gattatcgat tatcactgcc atttgaatcc ggaatacatt
     3061 gcgaaagacc ataagttcga caacctgggg caaatttggc tcgagggtga ccattacaaa
     3121 tggcgtgcga tgcgcaccaa cggtattgat gaaaaatact gtacaggtaa aaaaacttcg
     3181 gactgggaaa agtacgaaaa atgggttgaa acagtgcctt acaccatgcg aaaccccctt
     3241 taccactgga ctcacctgga actcaaacgg gctttcgggg tggatgaatt gctcaatccg
     3301 gcttccggaa agcggattta tgatatttgt actgcgcaac ttcgcacgcc ggaattttcc
     3361 gctcgcggac tgatgaaaaa attcaacgtg caggtagttt gtactacaga tgatcctgtg
     3421 gatacgctcg aacaccatat ttcgttgaaa aatgaaggtt tcgaaatcaa agtacttccc
     3481 acatggaggc cggataaggc aatggctgtt gaaaatccgg ccgaataccg gaaatatgtg
     3541 gaaaaattaa gcgaagtttc gggcgtttcc atttcaaatt ttgccgattt attgcaggcg
     3601 tt
//
LOCUS       DAORKP010000191         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7305_length_3602_cov_7.566958, whole genome shotgun sequence.
ACCESSION   DAORKP010000191 DAORKP010000000
VERSION     DAORKP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_3602_cov_7.566958"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLN75_08710"
     CDS             <1..335
                     /locus_tag="PLN75_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04236.1"
                     /translation="LAGLLNTIFPILIYKKLLSVVKDKQFISSIRLVTGIFAVPIFYL
                     LQFALVGLIGGSWLWALTYLLASPVVFYFGCYWRKGWKEIMRMEEVRQFVKEKKDVWE
                     KVLNAFEV"
     gene            complement(492..827)
                     /locus_tag="PLN75_08715"
     CDS             complement(492..827)
                     /locus_tag="PLN75_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04237.1"
                     /translation="MDNSEKTYNAVLDTEPRAQQVDNELTVPSLPFCILMDAIGMASY
                     IFPGVGESFDLVWAPISGFIFMKSFGGMTGKIGGLISMVEEAVPFIDIIPTFTIGHFY
                     AKYQSRKLK"
     gene            985..1788
                     /gene="mazG"
                     /locus_tag="PLN75_08720"
     CDS             985..1788
                     /gene="mazG"
                     /locus_tag="PLN75_08720"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRG04238.1"
                     /translation="MNKTYKHHTNEEKLAEFERLLAIMKRLRAECPWDREQTFESIRS
                     NTVEEAYELSDAILSRDMHEIKKELGDLLLHIVFYSEMGSETEDFDIYDVCKSINEKL
                     IFRHPHVFGEVNVNGDTAKVLENWENLKLKEKDGNKTVLGGVPASLPTLIKAYRIQEK
                     AASVGFDWEYREQVWDKVKEEIAEFQAEADKMDSDKMEDELGDVFFSLVNAARLYNIN
                     PDTALERTNQKFIQRFNFLEAETITKGRNLKEMTLAEMDEIWERAKKDV"
     gene            1937..>3602
                     /locus_tag="PLN75_08725"
     CDS             1937..>3602
                     /locus_tag="PLN75_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRG04239.1"
                     /translation="MLYPTTFEQKISFDTIRVRLKGYCLSSLGEEEVDKMGFSSHFQQ
                     ITYQLNLTNEMLQLLSSESDEMPVGDFFDIRSALSRVQAEGMYLDESEIFDLKRTLEA
                     VQKLVSFISKQKAEIFPNLRLLIAEVHSFSLIIKQIDNIINKFGKIKDNASPELARIR
                     KDIFHTQSGISKTLNAILRQAQADGYVEKDVSPTIREGRLVIPVSPAFKRKVSGIVHD
                     ESATGKTVFIEPQQVVEANNRVRELENEERREIIRILVEFTNFLRPHFQDIMQSQYFL
                     GKIDFLRAKSLFAKELKAIKPNVDNNCQMEWSRAFHPLLFLSLKKQNREVVPLDIVLN
                     ENQRILIISGPNAGGKSVCLKTVAILQYMLQCGMLIPVHENSRCGIFQQLFIDIGDEQ
                     SIENDLSTYSSHLLNMKFFIKNSNHKTLILIDEFGSGTEPQIGGAIAEATLERLNRNK
                     TFGVITTHYTNLKHYAEATQGIVNGAMLYDRQHLQPLFQLSIGNPGSSFAVEIARKIG
                     LPEDLIADATEKVGAEHMDYDKHLQDIVRDKRYWERKRQEIRLKEKR"
BASE COUNT         1157 a          749 c          752 g          944 t
ORIGIN      
        1 cccttgccgg actgctaaac acaattttcc cgattctgat ttacaaaaaa ctattgagcg
       61 tcgtgaaaga caagcagttt atcagttcca tccgcctggt aaccggtatt ttcgctgtgc
      121 cgatttttta tttgttgcag tttgcgttgg tagggctgat tggcggtagc tggctgtggg
      181 cattgacgta tctgctggct tcaccggtgg tgttttattt tgggtgttac tggcggaaag
      241 gatggaaaga aataatgcgg atggaagaag tcagacaatt tgtaaaagag aaaaaagacg
      301 tttgggaaaa ggtattgaat gcttttgagg tttagtgtgt cagttttttt tcaagacgat
      361 tttttaaaaa aaacaaacat tgccaaagtc ctcatatttt ggcaatgtta tattcgtatt
      421 ttaatcccat ccattcaaaa atcccccggg gacactgtac aaattcgaag tacgggggat
      481 tgagggtatt attatttcaa tttcctactc tggtattttg cataaaaatg cccgatcgta
      541 aacgttggaa ttatatcaat aaacggtaca gcttcctcca ccatggagat taaaccaccg
      601 atttttccgg tcattccgcc aaacgatttc ataaaaataa aacccgaaat tggcgcccac
      661 accaaatcaa aagactcacc aacacccggg aaaatataac tcgccatccc tatcgcatcc
      721 atcaatatac aaaacggaag cgaaggcaca gtcagctcat tatcaacctg ttgtgctctc
      781 ggttctgtat cgagcacagc gttgtaagtt ttttcagaat tgtccattta gcaataaaat
      841 taaaattgtt gataaaaaaa tgattttgaa ttgaacatca cttattattc ataaatcgtg
      901 ccgcgtttag ttaatttatt tgagtgacac aaaactaacc tattaaaatt atattcgtaa
      961 ctttgcaaat aaaatttttc cataatgaat aaaacataca aacaccatac aaacgaagag
     1021 aaactggctg aatttgagcg acttttggca attatgaagc gacttcgggc agaatgtccc
     1081 tgggatcggg aacagacttt cgagagcatc cgcagcaaca ccgtggaaga agcatacgaa
     1141 ctttcagatg cgattttaag ccgggatatg cacgaaatca aaaaagagct cggagatctg
     1201 cttctgcaca tcgtttttta ctccgaaatg ggcagcgaaa ccgaagattt tgacatttac
     1261 gacgtctgta aatccatcaa cgaaaagctg attttccgcc atccgcacgt gtttggcgaa
     1321 gtgaatgtaa acggcgatac agcaaaagtg ctggaaaact gggaaaacct gaaactgaaa
     1381 gaaaaagacg gcaacaaaac cgttttgggc ggtgttcctg cctcacttcc cacacttatc
     1441 aaagcgtatc ggattcagga aaaggcggca agcgtgggct tcgactggga ataccgcgaa
     1501 caggtttggg acaaagtgaa agaggaaatt gctgaatttc aggctgaagc cgacaaaatg
     1561 gacagcgaca aaatggaaga tgagttgggt gatgtttttt tcagtctggt aaatgctgcc
     1621 cgtctgtaca acatcaatcc cgacaccgca ctcgaacgaa ccaaccagaa attcatccaa
     1681 cgtttcaact tcctggaagc tgaaaccatt accaaaggcc ggaatttgaa agaaatgact
     1741 cttgccgaaa tggatgaaat ttgggaaagg gcaaaaaaag acgtctgaac tgtgatttat
     1801 ttgatttatt tgattttaat gatagtacaa acacatcata tgagattttt tttatcaatc
     1861 aaatcataat caaaaacgca gaatcaattt tttcccaatc gtatatcgta aatttgtaaa
     1921 tcgtaaattt cttcagatgc tctaccccac cactttcgaa caaaaaattt ccttcgatac
     1981 cattcgggtc cgattgaaag gatactgcct gagttcgctt ggcgaagaag aagtagataa
     2041 aatgggtttt tcaagtcatt ttcagcagat tacatatcaa ctgaatctca caaacgaaat
     2101 gcttcaactt ctttcatccg aatcggacga aatgccggtt ggagattttt ttgatattcg
     2161 aagtgcgctt tcaagagttc aggcagaggg aatgtatctg gacgaaagtg aaatttttga
     2221 cctgaaacga acgctggaag ctgtgcagaa actggtgagt tttatcagca aacaaaaagc
     2281 tgaaattttt ccaaatcttc gtctgctgat tgctgaagta cattcatttt cactgattat
     2341 caaacagata gacaacatta tcaataaatt cggcaaaata aaggacaacg catcgcccga
     2401 acttgcccgt atccggaaag acatatttca tactcaaagc ggcatctcaa aaacgctgaa
     2461 cgccattctg cgtcaggctc aggccgatgg atacgtggaa aaagacgtat cacctactat
     2521 acgcgaagga cggcttgtaa tcccggtttc gccggcattc aaacgtaaag tgagcggaat
     2581 tgttcacgat gaaagtgcca cgggaaaaac cgttttcatt gagccacagc aagtggtgga
     2641 agcgaataac cgcgtacgcg aactggaaaa cgaggaacga cgcgagatta tccgaattct
     2701 ggtggagttt accaatttcc tccgtcctca ttttcaggac attatgcagt cacagtattt
     2761 tttgggaaaa attgactttt tacgggctaa gtcgctattt gccaaagagt taaaagccat
     2821 caaaccgaat gtggataaca actgtcaaat ggaatggagc agggcgtttc acccgctgct
     2881 atttctctca ctgaaaaaac aaaacagaga agtggtgccg ttagacatcg ttctgaatga
     2941 aaatcaacgg attctgatta tttccggccc caacgccggc ggaaaatcag tttgtctgaa
     3001 aacagtggca atccttcaat atatgttgca gtgcggcatg ctgatacccg ttcacgaaaa
     3061 cagccggtgc ggaattttcc aacaactatt tattgacatt ggcgatgagc aatccattga
     3121 aaatgacctc tctacgtaca gttcacatct tctgaacatg aaattcttta tcaaaaacag
     3181 caatcataaa acactgattt tgatagatga atttggcagc ggcaccgagc ctcaaattgg
     3241 tggagccatt gctgaagcca cactcgaaag actaaatcga aacaaaacat tcggcgtaat
     3301 cactacccat tacaccaatc tgaaacacta cgcagaagcc acccaaggca ttgtaaacgg
     3361 cgctatgctc tatgaccggc agcatttgca accgcttttt caactttcca ttggcaatcc
     3421 cggaagttcg tttgccgtgg aaattgcccg gaaaatcgga ttacccgaag atttaattgc
     3481 cgatgccacc gaaaaagttg gagcagaaca catggattac gacaagcatt tacaggacat
     3541 agttagagat aaacgctact gggaacgcaa gcgacaggaa atccgactca aagaaaaacg
     3601 cc
//
LOCUS       DAORKP010000192         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7455_length_3564_cov_6.688230, whole genome shotgun sequence.
ACCESSION   DAORKP010000192 DAORKP010000000
VERSION     DAORKP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_3564_cov_6.688230"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..2832
                     /locus_tag="PLN75_08730"
     CDS             490..2832
                     /locus_tag="PLN75_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRG04240.1"
                     /translation="MKLKLLLLALAACGTLSIGAQNHTTGHPADETNYGYLKNYLPLK
                     QYINYGQYPNFKLGLAVGANDYLNNSTVKAVVNDYFTETVAGNEMKMSSCVSSNGTMN
                     FGTVTNFVNTAVGAGVGVYGHTLAWHSQQAVGYLNGLIKDLPAQPIEGSDTIVWSLLK
                     AKDFTQEKSIGWSSDKTTYGYTTSFVSDGLQVHTTRKVNSWEVQYIVMDNIPTVQGVT
                     YKMTVTVKGSAAGNIHSKLGGWSGGASAEIPFTTEWRDVVINYTSTLDNSFLLFQNGD
                     FVGDIWIKNIRFEKSVQGKKATRSCIVMNATAKNANTWDNQFWIKLGSFNQGDTYEFS
                     AQVRADNEAKVSTQLHNAPGSYVNYQALGDVNFTTEWKTVTKTGSFSNPAQSIAFNLN
                     EYAGANAYYFDNISLKVNGVERVINGNLDGTDVSSFAWRHYGDNAVTNATIKTDYLYV
                     QLPQGIPLSAQAKHDTLVYAMDRWIGGMMTACGGNVKAWDVVNEAISGGDSNGDGVYE
                     LQHANGDDSNFFWQDYMGDLEYTRQAVRLARLHYANSMASKGGDDGKLTLFVNDYNLE
                     SDWDGNGKLKSLIKWIERWEADGVTKIDGIGSQMHISCYMNESTQTSKRNAIENSFRL
                     MAATGKLVRISELDMGMVDASGKDVPTANMTEEMHQRMSAFYEWIVKKYLEIIPASQQ
                     WGICQWCATDAPSNSGWRANTPVGLWTLDWYRKHTYAGFARGLGAPQNPTGIDVIEDG
                     LNKPDALSPIHDIYGRYAGKDFESLPAGLYIQNGKKYLKR"
     gene            2878..>3564
                     /locus_tag="PLN75_08735"
     CDS             2878..>3564
                     /locus_tag="PLN75_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04241.1"
                     /translation="MKNNFISKKPLVMLLFAAMLLPAGTPAKAQTGKKASSPTQFVHR
                     VGGNPYLPLWEHLPDGEPRVFEDPDNPGKYRVYIIGSHDVRFGSYCGPDIRAWSAPQE
                     DLTNWRDEGPIFTYPIEGQWDVMYAPDLVEIKRKDGKKEYYLYPHSRGRNREAMVCKG
                     DRPTGPFKPINMNEDGTKTIPGSIFGFDPSVYVENITNPNDPDYKIGFRAYGFWGFQR
                     SSAAQLDQSTM"
BASE COUNT         1089 a          761 c          855 g          859 t
ORIGIN      
        1 tggtttaaat atgtaataga taagtgttat ataaggttta taatttaagg aagtattacg
       61 cagagattgt tgaagcagca gggattgaca aaccttaaat agaagaattc acagtcctcc
      121 cgaataagta ctttcaggaa gtgaaaggaa tgaaacaact tttgcaaaca atattaaaag
      181 caggaacttt tatcacagct ttgacaaagc gggacgccat gccgggcatc ggatgcaggt
      241 tgatttgcag gcttcggaga acgggatatg aataattgca aagaattatc caaattcaaa
      301 catcctaccc caataattat ctaaatttaa acacctcacc gacagatcaa taatgacaag
      361 ttaataaaaa tagcgtatat ttgagcgtat ttcttgtggg ggaaaaccat cttaatttca
      421 atgaagaaat gagaaaacat aaaccttaaa acgaatcatt cattacccga aataatacta
      481 taataaaata tgaaactaaa attactctta cttgctttag ccgcgtgcgg caccctgtcg
      541 attggtgcgc aaaaccacac aaccggtcat ccggcagatg aaacgaatta cggatacctg
      601 aagaattatt tgccactcaa gcaatatatt aattatgggc agtatcctaa cttcaaactg
      661 ggacttgctg tcggtgccaa tgattacctg aacaacagca cggttaaggc ggttgtcaat
      721 gattatttca cggagactgt tgcaggcaat gaaatgaaaa tgtcgagctg tgtgagcagc
      781 aacgggacca tgaatttcgg taccgttact aattttgtaa atacggcagt cggagccggt
      841 gtaggtgtgt acggacatac gctggcatgg cattcgcagc aggcagtggg ctatctgaac
      901 ggtttgatca aagacctgcc cgcacagccc atcgaaggga gtgacacgat tgtttggtcg
      961 ttgctgaaag caaaggactt cacacaggaa aagagcatag gttggtcatc ggacaaaaca
     1021 acctatggct ataccacctc ttttgtgagt gacggtcttc aggtgcatac caccaggaaa
     1081 gtcaactcat gggaggtgca gtacattgtt atggacaata tcccgacggt tcagggcgtg
     1141 acctacaaaa tgactgtcac cgtaaaagga tcggctgccg gcaatatcca cagcaaactt
     1201 ggaggctgga gcggcggagc aagcgcagaa atacccttca caacggaatg gagagatgtg
     1261 gttatcaact atacaagcac actcgataac agttttctgc ttttccagaa cggcgacttc
     1321 gtaggtgata tctggatcaa gaacatcagg ttcgaaaagt cggtgcaagg caagaaggct
     1381 acccgtagct gtatagtgat gaatgcgacg gctaagaatg cgaatacatg ggataaccag
     1441 ttctggatca agttgggaag tttcaatcag ggtgacacct atgagttctc ggcccaggtg
     1501 cgtgcagaca acgaagcgaa ggtttccact caattacaca acgctccggg cagttacgta
     1561 aactatcagg cccttggtga tgtcaacttc acaacagaat ggaagactgt gaccaaaacc
     1621 ggatcattct ctaatcctgc acagtccatc gctttcaacc tcaacgaata tgcgggtgcc
     1681 aacgcttact atttcgacaa tatcagttta aaagtaaatg gagtggaacg tgtgataaac
     1741 ggcaatttgg atggaaccga tgtttcatcg tttgcatgga gacactatgg cgataacgca
     1801 gtgacaaacg ctaccataaa gacagactac ctctacgttc aactgcctca gggcattcca
     1861 ttgtccgctc aggccaaaca tgacaccctt gtttatgcta tggaccgttg gattggcggt
     1921 atgatgactg cctgcggcgg taatgtaaaa gcctgggatg tggtgaacga agcgatatcg
     1981 ggaggtgatt cgaacggtga tggtgtgtat gaacttcagc atgccaatgg cgacgacagc
     2041 aatttcttct ggcaggatta catgggcgac ctggaatata cccgtcaggc agtacgtttg
     2101 gctcgtctgc attatgccaa ttcaatggct tcgaaaggtg gtgacgatgg taaactgacg
     2161 ctgtttgtga acgactacaa cctggaaagc gactgggacg gcaacggcaa actgaaatcg
     2221 cttattaagt ggatagaacg ttgggaagcc gacggcgtaa caaaaataga cggcatcggc
     2281 tcgcagatgc acatatcgtg ctatatgaat gagtccaccc aaaccagcaa gaggaatgct
     2341 attgagaaca gtttcaggct aatggcagct acaggcaaac tggttcgtat cagcgaactg
     2401 gatatgggaa tggtagatgc cagcggcaag gatgtgccta cagccaatat gacagaagaa
     2461 atgcatcagc gcatgtcagc cttttacgaa tggatagtga agaaatacct cgagataatt
     2521 cccgccagcc aacagtgggg tatctgccaa tggtgtgcaa ccgatgctcc gtcgaacagt
     2581 ggctggcgtg caaatacacc tgtaggactg tggacactgg actggtaccg caagcatacc
     2641 tatgccggtt ttgcacgcgg tctgggggca cctcaaaacc caacaggcat tgatgtcatc
     2701 gaggatggtt taaacaaacc ggatgcatta tcccccattc acgatattta tggacgctac
     2761 gcaggcaaag atttcgagtc actccctgca ggcctttata tacaaaacgg taaaaaatac
     2821 ctgaaacgct gatcgtttaa tgaaaatcat atatttagtt atacaaatca tcaaaaaatg
     2881 aaaaacaatt ttatttcgaa aaaaccatta gtcatgctat tatttgcagc tatgttgttg
     2941 cctgccggca ctccggcgaa agcgcagacc ggcaagaaag catcgagccc gacacagttt
     3001 gttcatcgtg ttggtggaaa cccttacctg ccgttgtggg aacatttgcc cgatggcgag
     3061 ccccgtgttt ttgaagatcc tgacaatccg ggaaaatacc gtgtgtatat tatcggatcg
     3121 cacgatgtac gttttggaag ttattgcgga ccggacatac gtgcatggtc ggcaccacag
     3181 gaagacctta ccaactggcg tgacgaaggt cctatcttta cttatcctat cgaaggacaa
     3241 tgggatgtaa tgtatgctcc cgatttggtg gaaataaaac gcaaagacgg taaaaaagaa
     3301 tattatcttt atccacatag ccgcggacgt aacagagaag ccatggtatg taaaggcgat
     3361 cgtcctacag gtcctttcaa acccatcaat atgaatgaag atggaacgaa aaccataccc
     3421 ggaagtattt tcggattcga cccttctgtt tatgttgaaa atatcaccaa tccaaacgat
     3481 cccgattata aaattggttt cagagcttat ggtttctggg gattccagcg ttcatcagcg
     3541 gcccaactcg accagagcac tatg
//
LOCUS       DAORKP010000193         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7814_length_3470_cov_9.632504, whole genome shotgun sequence.
ACCESSION   DAORKP010000193 DAORKP010000000
VERSION     DAORKP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7814_length_3470_cov_9.632504"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..2260
                     /locus_tag="PLN75_08740"
     CDS             212..2260
                     /locus_tag="PLN75_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRG04242.1"
                     /translation="MKKLTLFFMISILFACSGNKSETQSNTPPIDSFDYKVDTFADIK
                     ILRYRVPGFENLTLKQKEMIYYLSQAATEGRDILWDQNNKHNLCIRRTLEAVYENTAD
                     KTSDDFKKLETYLKRIWMANGIHHHYSEDKIMPAFTAGFFADAVKSIDSSKLPLEQGE
                     TVEQLIAKLTPILFDPNVMPKKTNQEAGVDLITTSANNYYEGVTQKEVEDFYGKMKNP
                     NDSTPISYGLNSKLTKENGQVVEKVYKVGGMYGAAIERIVGWLEKAAGVAENGHQKEV
                     INTLIRFYRNGDLKTFDEFNIKWVQDLTSQIDFVNGFTENYGDPLGMKASWESLINFK
                     DPEATKRTEIISQNAQWFEDNSPTDKKFKKETVKGVSAKVINAAMLGGDCYPSTPIGI
                     NLPNANWIRAKYGSKSVTIQNITEAYDQASLGNGFNEEFMWSDTERERAAKYGSLTNN
                     LHTDLHECLGHGSGKLLPGIDPDALKAYGSPIEETRADLFGLYYLGDPKLVELGLLPD
                     AEAYKAQYYQYLMNGFMTQLTRIQPGKNIEQAHMRNRQLIASWVFEKGKAENVVELKE
                     KDGKKYIVINDYEKVRNLFGELLAQIQRIKSTGDFAAGRDLVEKYAVKVNQDIHKEVL
                     ARYAKLNIAPYSGFVNPVYTLVTDKNGKITDVNISYNEGYIQQHLRYSKDYSVLPTYN
                     "
     gene            2324..2764
                     /locus_tag="PLN75_08745"
     CDS             2324..2764
                     /locus_tag="PLN75_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HRG04243.1"
                     /translation="MKQKLFLLLTVVAITFSACKKADSKEEQPVNDSIEMTSEVPEGH
                     TSNIAVDWEGEYKGIIPCEDCDGVETSLILNYDSTFTMTKTYIGKGGPYESKGSFSRA
                     ASGNIIVLKFDDASEAKYQLGDGSLSLLNDDGSIIEGEDYVLRK"
     gene            complement(2879..>3470)
                     /gene="coaE"
                     /locus_tag="PLN75_08750"
     CDS             complement(2879..>3470)
                     /gene="coaE"
                     /locus_tag="PLN75_08750"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRG04244.1"
                     /translation="KPFIIGITGGIGSGKSTFSRYLMRRGQLVYDTDLEAKILQNTNK
                     DLRRAIMDEFGEDVYNAAGLDRTKMAGIVFSDSAKLHRLNKLVHPVVINDFQNWVNEN
                     ADRKFLFMECAILFEGGFRSLVDKVVVVTAPEEVRVQRVMLRDCQTEAAVKARVRNQM
                     QDAEKIKLADWVFDTNNDIFPHERVDEFLEMLKDSL"
BASE COUNT         1099 a          782 c          728 g          861 t
ORIGIN      
        1 ttttaatgtt acaaatttac taacactcta ataataaaac aaatagaaat taacaatagt
       61 tgtatttaca aaaccgataa cacataaaat cttttgaaaa ctgcacaatt ggatgcgtta
      121 ttaaaagcct gttctattaa tatttcgtaa ctttgtatcc aattttcaaa cctcaatgtc
      181 tgatttttca cgaaaaaaaa acgaaaacaa tatgaagaaa ctaactttat tctttatgat
      241 ctccattcta tttgcctgta gcggaaataa atccgaaact caatccaaca caccacccat
      301 cgacagtttt gattacaagg tggatacctt tgccgatatt aaaatactca ggtatcgtgt
      361 tcccggcttt gaaaatctga ccctgaaaca gaaagaaatg atttattacc tttcgcaggc
      421 agctaccgaa gggcgcgata ttctctggga tcaaaacaac aaacacaacc tgtgcattcg
      481 tcgcacgctg gaagctgtat atgaaaacac tgcggataag acttccgatg attttaagaa
      541 actcgaaacc tacctgaaac gtatctggat ggctaacggc attcaccacc actattcgga
      601 agataaaata atgcctgctt tcacagccgg tttttttgct gatgcagtaa aaagcatcga
      661 cagttcgaaa ctgcctctgg agcagggtga aaccgtagaa caactgatag caaagctcac
      721 gcccattctt tttgacccga acgtgatgcc taaaaagacc aaccaggaag ccggcgtgga
      781 tctgataacc acttctgcca acaactacta cgaaggagtc acgcaaaaag aagtggagga
      841 tttctacggt aaaatgaaaa atccgaacga ctccacaccc atttcttacg gattgaacag
      901 caaactgacc aaagaaaacg gtcaggtggt tgagaaggtg tataaagtag gcggtatgta
      961 cggtgcagca attgagcgca ttgttggttg gctcgaaaaa gcagccggag tagcggaaaa
     1021 tggccaccaa aaagaggtaa tcaataccct gatacgcttc taccgcaacg gtgatttgaa
     1081 aactttcgat gaattcaaca tcaaatgggt gcaggatctg acttcgcaga ttgattttgt
     1141 aaacggattt acagaaaact acggcgatcc gctgggtatg aaagcaagct gggagtcgct
     1201 catcaatttc aaagatcccg aagccaccaa acgcacagaa attatcagcc aaaatgcaca
     1261 gtggtttgaa gacaactcac caaccgacaa aaaattcaaa aaggagacgg taaaaggagt
     1321 ttcggccaag gtaatcaatg ctgccatgct cggcggcgac tgctatccat ccactcccat
     1381 aggcatcaac ctgcccaatg ccaactggat tcgggcaaag tacggttcca aatcggttac
     1441 tatccagaat atcaccgaag cttacgatca ggcatcgctc ggcaatggat ttaacgaaga
     1501 attcatgtgg tcggataccg aacgcgaaag ggctgcaaaa tacggttcgc ttaccaacaa
     1561 cctgcacacc gacctgcacg agtgcctcgg gcatggttca ggaaaactgc ttccgggaat
     1621 cgatccggat gcccttaaag cgtatggctc gcccattgaa gaaacccggg ccgatctttt
     1681 cggactgtat tacctgggcg acccaaagct cgtagaactc ggactcctgc ccgatgccga
     1741 agcttacaaa gcacaatatt atcagtacct gatgaatgga ttcatgaccc aacttacccg
     1801 cattcagccc ggcaaaaaca ttgagcaggc acacatgcgc aatcgtcagt tgattgcctc
     1861 ctgggttttc gaaaaaggaa aagctgagaa tgtagtggaa ctgaaagaaa aagacggcaa
     1921 aaaatacatt gtgatcaacg actacgaaaa agtgcggaat ttattcggtg aactgcttgc
     1981 ccaaattcag cgcatcaaat caaccggcga ttttgcagcc ggaagggatt tggttgaaaa
     2041 atatgccgta aaagtaaatc aggatatcca caaggaagtg ttagctcgat acgcaaagtt
     2101 gaatatagct ccttacagcg gttttgtgaa tccggtttac acgcttgtga ccgacaaaaa
     2161 tggcaagatt accgatgtaa acatttcgta caacgaaggg tatattcagc agcatctacg
     2221 ttattcgaaa gattattcag ttttacctac ttataattag gatatcggac attcctgtcc
     2281 gaaaaacagg caggaatgcc tgttctccta aatttttata aaaatgaaac aaaaattatt
     2341 tttactgctc accgttgttg caataacctt ttctgcttgt aaaaaagctg attcgaaaga
     2401 agagcaaccg gtaaatgatt ccattgaaat gacttctgaa gttccggaag gacatacatc
     2461 caacattgct gttgactggg aaggagaata caaaggaatc attccgtgtg aagactgcga
     2521 tggagtggag acatcactga ttctcaacta cgacagcacg tttaccatga ccaaaaccta
     2581 catcggcaaa ggcggacctt acgagtcgaa aggatctttc agccgcgcgg cgtcaggaaa
     2641 tattatcgtt ctgaaattcg acgatgcaag tgaagccaaa taccagcttg gtgatgggag
     2701 tttatcgttg ctcaacgatg acgggagtat catcgaaggc gaggattacg ttttgagaaa
     2761 ataagagcga aaaaaacatt tcccccggga cttcataaac aaggccgact ggttcgaaat
     2821 gaatcagacg gcctatgtat ttaattgaat taacgtgaac gttcttcagg cgattttttt
     2881 ataaagaatc tttaagcatt tccaaaaact catccacccg ttcatgcggg aaaatatcgt
     2941 tgttcgtgtc aaacacccag tcagcaagct taattttttc tgcatcctgc atctgatttc
     3001 ttacacgggc tttgacggct gcttccgttt ggcagtctct gagcataaca cgctgaacac
     3061 gaacctcttc gggagcagtc actaccacca ctttatcaac cagcgatctg aaaccaccct
     3121 cgaaaagtat ggcacattcc atgaaaagaa atttccggtc agcattttca tttacccagt
     3181 tctgaaaatc gttgataaca accggatgaa ccagtttatt cagccggtgg agttttgccg
     3241 agtctgagaa tacaatgccg gccattttgg tgcggtcaag cccggcagcg ttatatacat
     3301 cttcaccgaa ttcgtccata atggcacgcc gtaagtcctt gttggtgttc tgaagaatct
     3361 ttgcctccag atcggtatca tacaccaact gcccacgacg catcaggtag cgtgaaaaag
     3421 ttgattttcc gcttccaatc cctcccgtga tgcctatgat gaacggtttt
//
LOCUS       DAORKP010000194         3450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7891_length_3450_cov_9.065685, whole genome shotgun sequence.
ACCESSION   DAORKP010000194 DAORKP010000000
VERSION     DAORKP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7891_length_3450_cov_9.065685"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PLN75_08755"
     CDS             <1..1253
                     /locus_tag="PLN75_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRG04245.1"
                     /translation="IRRIYRIDTPEIIFDDEAIRKLVTNLLSNAIKFTPDNGSITLYV
                     SKLQDVQSMQEKLYINLKDNGFGIDEHEMEQIFERFYQSKKHEKLSVSGQSGTGIGLY
                     LCQKIVELLGGTIKAKNNRGGGASFRVLLPLVRGNTDMPTEIKEYKNVPDAGVDNQVT
                     APYLRDAKSTILVVEDNADMRKYICSILSDQYKILEAENGEEALHLLKSRTIDFVVSD
                     LMMPVMDGMELSKQVKSDFAISHIPFLMLTAKSNVETRISSYKSGVDEFLAKPFDADL
                     LLARITNMLEARRNYQRKFSMNMDLKELNFSEESADEKFIRQALDIVKKNYKNPDYEV
                     TDFIKEMGVSKSLLNKKMNILTGQPAVQFIRNYRLTVAHELILKQKGNLNISEIAFEV
                     GFNDPKYFTRCFTRYFGIAPSMVK"
     gene            complement(1413..2711)
                     /locus_tag="PLN75_08760"
     CDS             complement(1413..2711)
                     /locus_tag="PLN75_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRG04246.1"
                     /translation="MKQLDINFMHPVEQINIIIGRIYRSGMTTTSGGNISIRDTNGDI
                     WITPSGVDKGNLTTKDIMCVKKDGTVVGPHKPSSEFPFHKAIFEARPELKAVIHAHPP
                     ALVAFSIARTVPDTKIVPQAHNICGDIGFAEYGCPGSEDLGKKIAKQFRNTNYKAVIM
                     ENHGVVLGGTDMMDAYQRFETLEFCCRTIINAKKLGGVNYLTDEEVSKYAHHIPANVP
                     HFMDISYPSDERAIRSDMVKIIRRACNQGLMISTYGTVSVRWKNDDFLITPRDVARWD
                     IMPEDIVQIKNGMAEAGKIPSRSVALHQRIYQLNPHINSIICTQPVNLMAHAVSGKKF
                     DVRTIPESWIFLQDVPSIPFGNIYKEIDEVAQMFADNRVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMATSIGELKPMGDKEIDELRIAFNVK"
     gene            complement(2878..3276)
                     /locus_tag="PLN75_08765"
     CDS             complement(2878..3276)
                     /locus_tag="PLN75_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRG04247.1"
                     /translation="MNKVYAPISDKEEEIGKAIVHAAYIVHKELGPGLLEKVYEICFA
                     HTLQKMGYNVKRQLDIPIVFDGITFSEGLRLDVIVNDLVICELKALETVNPLWEAQII
                     SHLKLTGKRLGYLINFNVPYIKDGIRRFVK"
     gene            complement(3269..>3450)
                     /locus_tag="PLN75_08770"
     CDS             complement(3269..>3450)
                     /locus_tag="PLN75_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04248.1"
                     /translation="PLTPISEFEKLGENSEFFKNLAEICHRHYGLWSVEAETYLLENN
                     NRHQVSKTQRSTKNE"
BASE COUNT         1005 a          758 c          690 g          997 t
ORIGIN      
        1 atatccggcg aatctatcgg atagacactc ccgaaatcat attcgacgat gaagccatcc
       61 gcaaactggt taccaatctg ctttccaatg ccataaaatt cacacctgac aatggttcga
      121 taacactgta tgtaagtaaa ctgcaagacg tgcaaagcat gcaggaaaag ctgtatatca
      181 atctgaaaga caatggtttc ggaatcgacg aacatgaaat ggagcaaatt ttcgaacgat
      241 tctaccaatc caagaaacac gaaaaacttt cagtgtcggg gcaaagcgga accggaattg
      301 gtttatacct gtgtcagaaa attgtagaat tgctcggcgg aaccatcaaa gccaaaaaca
      361 acagaggcgg cggtgcttct ttcagggtac tgctgccact ggtgaggggc aacacagata
      421 tgccaaccga aataaaagaa tacaaaaatg taccggatgc aggagttgac aatcaggtta
      481 cagcacctta tcttcgcgat gcaaaatcca ccattcttgt ggtggaagac aacgctgata
      541 tgcgaaaata catttgctcc atcctttccg atcaatataa aatacttgaa gccgaaaatg
      601 gtgaagaagc actgcatttg ctcaaatcca ggaccatcga ttttgtagtc agcgatctga
      661 tgatgcccgt gatggacggt atggaattat caaaacaggt aaaatccgat ttcgccattt
      721 cacacattcc gtttctgatg ctcaccgcca aatccaatgt ggaaacccgg attagcagtt
      781 ataaatcagg agtcgatgag tttcttgcca aacctttcga tgccgatctg ctgcttgcac
      841 gcatcaccaa catgctggaa gcccggcgca attatcagcg gaaattcagc atgaatatgg
      901 atttgaagga acttaatttc agcgaagaat cggccgatga gaagttcatc cgacaggcac
      961 tggacattgt aaaaaagaat tacaaaaatc cggattacga agttactgat tttataaaag
     1021 aaatgggagt aagtaaaagt cttctgaata agaaaatgaa cattctcacg gggcagccgg
     1081 ccgtacagtt tatccggaat taccgtctga cagtagctca cgaactgatt ctgaaacaaa
     1141 aaggaaacct caatatctcc gaaattgcgt ttgaagttgg tttcaacgat cctaaatact
     1201 ttacaaggtg ctttacgcgg tattttggca tagcaccaag tatggtcaaa tagaacagcc
     1261 ccctaaatcc cccttggggg acttccgggg gattgcaaca gagaatcatt tccttatttt
     1321 aattcacgtg cgcagcaccg attctaaagt tattcaagtt ggaggatttg aggtgaataa
     1381 ctccccctcc aagagattgg aatgatactg acttacttca cattgaacgc tatacgaagt
     1441 tcatctattt ccttgtcacc catgggtttc agttctccga tggaggttgc cataaccagc
     1501 gaattggccg agaattcggc tacttccaga taatcaaacg tattaagcaa tttatcgccg
     1561 gtgacaaaaa cgctgtcgtt ttcaaccagg atcacccggt tatcggcaaa catctgagcc
     1621 acttcatcta tttctttgta aatatttccg aacggaatgg acggaacatc ctgcaggaaa
     1681 atccagcttt cgggtatggt gcgcacatcg aattttttgc cgctcacggc gtgtgccatc
     1741 agattaaccg gttgtgtgca gatgatggaa ttgatatgcg gatttaactg ataaatccgc
     1801 tgatgcagcg ctaccgaacg gctcgggatt tttccggctt cagccattcc gttttttatc
     1861 tgtacaatat cttcgggcat gatgtcccat cgtgccacat cccggggtgt aatcagaaaa
     1921 tcatcattct tccatcttac agaaaccgtt ccatacgtag aaatcatcag cccctgatta
     1981 caggcacgtc gtattatttt caccatatct gaccgtattg cgcgttcatc ggaaggatac
     2041 gaaatatcca taaaatgagg cacattggca ggaatgtgat gagcatattt tgacacctcc
     2101 tcatctgtca gatagtttac tccgcccagt tttttggcat tgatgatagt acggcaacaa
     2161 aattcgagcg tttcgaaacg ttgatatgcg tccatcatat ccgttccacc caataccacg
     2221 ccgtgatttt ccataatcac cgctttataa ttggtatttc tgaactgttt ggcgattttt
     2281 ttacccagat cttcacttcc gggacatccg tactcggcaa aaccaatatc accacagata
     2341 ttatgtgcct gaggaacaat cttggtatcg ggaacggtgc gggcaatact gaaagccacc
     2401 aatgccggcg gatgtgcatg aattaccgct ttcaactccg gacgggcttc aaaaatggct
     2461 ttgtgaaacg gaaattccga cgaaggtttg tgaggaccta ccaccgttcc gtcttttttc
     2521 acacacataa tgtctttggt ggtcaggtta cccttatcca cgcccgaagg agtgatccag
     2581 atgtcgccgt tagtatctct gatagagata tttccacccg aagtggttgt catgccgctt
     2641 ctgtaaattc gtccgatgat gatgttgatc tgctccaccg gatgcataaa gttaatgtca
     2701 agttgtttca tatttttagc cacaaaggca ataaggcgca aagttgcact aagttattta
     2761 tttttatgta aatgtaataa tttcctgact ttgacaatgc atcacccaaa atgattttgc
     2821 cacaaaggca ataaggcaca aagttgcact aagttatttg tttttatgta aatgtaatta
     2881 ttttacaaac cgtcttatgc cgtcttttat atatggcaca ttgaaattta ttaaataccc
     2941 aagtcgtttt ccagttagtt ttagatgact gattatctga gcttcccata atggatttac
     3001 tgtttccaat gcttttaatt cacaaatgac caagtcatta actataacat ctaatctcaa
     3061 tccttcagaa aacgtgattc catcgaaaac aattgggata tctagttgtc tcttaacatt
     3121 atatcccatc ttttgtaatg tatgagcaaa acaaatctca tatacttttt ccaataaacc
     3181 cggtcctaat tctttatgga caatatatgc tgcgtgaaca atggctttgc caatttcttc
     3241 ttccttgtca gatatcggag cataaacttt attcattttt tgtgcttctt tgtgtctttg
     3301 atacttggtg gcgattatta ttttccaaca aatacgtttc tgcttccacg ctccaaagtc
     3361 cgtagtgccg gtggcaaatt tctgccagat ttttaaagaa ttcgctgttt tcacccagtt
     3421 tttcgaattc gctgattggt gtcaaaggca
//
LOCUS       DAORKP010000195         3430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_7992_length_3430_cov_9.163852, whole genome shotgun sequence.
ACCESSION   DAORKP010000195 DAORKP010000000
VERSION     DAORKP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7992_length_3430_cov_9.163852"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..2804
                     /locus_tag="PLN75_08775"
     CDS             78..2804
                     /locus_tag="PLN75_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRG04249.1"
                     /translation="MKKIKTLFLLFLCTTVLMMQAQQVTISPIPQSITWGAKAFNNTT
                     SYFLTGSETADQDAVDLLKSKLNTGTTGIEVIIGEKDDPVVANYVAQIPDFKEGYYLS
                     VSNDKVIIAGADESGTYYGVQSFLQIISVPEVMSVTIKDYPGVTERGIIEGFYGNPYS
                     HQARQRLFRFFGENKMNVYIYGPKDDPYHGFGTKWRDPYPTAQAAQMKELIAAAHKNK
                     VNFVWAVHPGNNISWTDNNKDGIVDDFVACKNKFQLMYDLGVRSFAVFFDDIGGVGAD
                     PVNQAKLMNYLTTEFVNKKTDVSPLLLCPTQYNKSWSSGTYLSILGTQMDSSVRIMWT
                     GNSVVDMINKTDLDWINAQISRKAYIWLNYPVTDYCVDHLLMGPTYGNDTNIASQLSG
                     FTANPMEYAEASKVALYSIADYTWNMSKYNSNSSWLRGLKYLMPQNYDAFKIFCENNI
                     DLGSTAHGLRRSGESAEFKTVADAFMTDFTVGKYNSAQINALAEQFQRFINSTNELTA
                     STNNPYLIEEIKPWMDVFNLMGQRGKLLLDMYEALNAEDSVAFINHYLKTDSLEIVQG
                     KIRSRDFSGSIKNPYPKPANEVVAPFLKQLKARLINNYRNKFDYRTDVFPTILLENGN
                     YYIKYNGKYLTNRNANASGGNPEFLADRDSINPQRQEWTVTIDPITERYSIRNTQDNR
                     YVNELGNFGVNSYEAIWHTYNLHRFSGKYAIQNAGSAGDKFWQSNATRISPSTDNLLS
                     SGNFLFEFVPVGQESALHPVITFGETYYVKFNDNFLTNNNPNGSGGNPTFRTYITTPD
                     SKTQKWIFTLDTTSDRVKLVNAADNRYVNELGAFGTNQYYPAWNSYVITELGGKFAIR
                     NAGDAGDKYWKAGTRIEAGDAGQADSYIFEIVSDKTATSVKKKFSQKGFLSH"
     gene            2837..2995
                     /locus_tag="PLN75_08780"
     CDS             2837..2995
                     /locus_tag="PLN75_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG04250.1"
                     /translation="MKTMQLSNLHGVRIASVNNSNVMEASCLASGIYILSVVSTNGTK
                     DTFKLLKP"
     gene            3051..>3430
                     /locus_tag="PLN75_08785"
     CDS             3051..>3430
                     /locus_tag="PLN75_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04251.1"
                     /translation="MRNLKTAKIGFLTGFILLTSAFVNAEDMLSGFRYSPEQAPKGNE
                     WESPENLALNKEQPRAWFFSFQDTESARKVLPENSAYWKSLDGTWKFNWVKHPDERPV
                     DFYKPEFNVNGWENIPVPVSWNIHG"
BASE COUNT         1069 a          712 c          704 g          945 t
ORIGIN      
        1 ttacaattaa tttagagaaa catattattt atattcgtac ctttgcattc atctaattac
       61 taacctttta ttgcaaaatg aaaaaaatca aaacactttt tctgctgttt ttatgcacaa
      121 cagttctgat gatgcaggca cagcaggtta ctatctctcc cattcctcaa tccatcacct
      181 ggggagctaa agcattcaat aacaccactt catattttct tacaggcagt gaaaccgccg
      241 atcaggatgc cgttgacctt cttaaatcaa aattaaatac tggtactacc ggcattgaag
      301 ttataattgg agagaaagac gatccagtcg ttgctaatta cgtcgctcag atacctgatt
      361 ttaaagaagg ttattatctt agcgttagta atgacaaggt cattattgcc ggtgccgacg
      421 aatcgggtac ttactacgga gtacagtcct ttttacagat aatttccgtt cccgaagtga
      481 tgtcggtaac aataaaggac tatcccggtg taaccgaaag aggtattatc gaaggttttt
      541 acggaaatcc atattcccat caggcccgac aaaggctttt ccgatttttt ggagaaaaca
      601 agatgaatgt gtacatatat ggccccaaag acgatcctta tcacggtttt gggaccaaat
      661 ggcgtgatcc ttatcccact gctcaggctg cacaaatgaa agaactgatt gctgccgcac
      721 acaaaaacaa agttaatttt gtgtgggcgg tgcaccccgg aaacaacata agctggacgg
      781 ataataataa agacggaatt gtggatgact ttgtggcctg taagaataaa ttccagttga
      841 tgtatgatct cggagtgcgt tcttttgctg ttttcttcga cgatatcggc ggagtaggag
      901 ccgaccccgt taatcaggca aagttgatga attatctgac cactgaattt gtaaataaga
      961 aaacagatgt ttcacctttg ctgctctgtc ctactcaata caataaatca tggtcaagcg
     1021 gaacttatct ttctatcctt ggtacacaaa tggattcaag tgtgcggatt atgtggacag
     1081 gcaacagcgt ggtagatatg atcaacaaaa ccgacttgga ttggatcaat gctcaaataa
     1141 gccgaaaagc atacatctgg ctcaactatc cggtgacaga ttattgtgtg gaccatctgc
     1201 tgatgggacc tacctacgga aacgatacaa atatagcatc acagctctca ggcttcacgg
     1261 ccaatccgat ggaatatgcc gaggcttcca aagtggctct ttacagcatt gccgattata
     1321 cctggaacat gtcaaagtat aattcaaaca gttcatggct gaggggtttg aagtacctga
     1381 tgccgcagaa ttatgatgct tttaaaatct tctgtgaaaa caacattgat ttgggctcga
     1441 cagctcacgg tttgcgtcgt agtggcgaat ctgctgaatt taagactgtt gcagatgcct
     1501 tcatgacaga ctttacagtc ggtaaatata attcggcaca aattaatgcg cttgctgagc
     1561 aattccagcg ttttatcaat tcaacaaacg aacttactgc ctctacgaat aatccttatt
     1621 tgattgaaga aattaagccc tggatggatg ttttcaacct gatgggacag cggggaaaac
     1681 tgctgttgga tatgtacgaa gccctgaatg ccgaagattc tgttgctttt ataaatcact
     1741 acctcaaaac tgattcgctg gaaattgtgc agggaaaaat tcgctcaaga gatttttcgg
     1801 gaagcattaa aaatccttat ccaaaaccag ctaatgaggt tgttgctcct ttcctgaagc
     1861 aactgaaagc tcgtttgatt aacaactacc ggaataaatt cgattaccgg acagatgttt
     1921 tcccgactat tttgcttgag aatggtaatt actatattaa atacaacgga aaatatctta
     1981 ctaaccgaaa tgcaaatgct tcgggcggga atcctgaatt tctggctgac agagattcca
     2041 tcaatccgca acgtcaggag tggaccgtaa ccatcgaccc cattactgaa cgatacagta
     2101 tccgtaatac acaggacaat cgctatgtaa atgaattggg aaatttcgga gtaaactcct
     2161 acgaagctat ttggcatacc tacaacttac atcgtttcag tggaaaatat gccatacaga
     2221 atgccggttc ggctggcgac aaattctggc agtcaaacgc tacacgcatc agcccaagca
     2281 ccgataatct gctatcctcc ggcaacttcc tgttcgaatt cgtacccgtg ggacaggagt
     2341 cggcacttca tcccgtaata acatttggcg aaacatatta cgtaaaattt aacgacaatt
     2401 ttctgacgaa taataacccg aacggatctg gtggcaatcc aactttcagg acgtatataa
     2461 caacgcccga ctcaaaaacc caaaaatgga tttttaccct ggatactact tcagaccgtg
     2521 taaaactcgt taatgcagcc gacaacaggt atgtgaatga attgggagct ttcggtacaa
     2581 atcagtatta tccggcctgg aattcctatg tgataaccga actgggtgga aaatttgcta
     2641 tcagaaatgc cggagatgcc ggagataaat actggaaagc cggtacacgc atcgaagcag
     2701 gtgatgcggg acaggcagat agctatattt ttgagattgt atctgataaa acagccactt
     2761 ctgtaaaaaa aaagttttct caaaagggtt ttctgtcgca ctaaataatg gatacctaca
     2821 ggttttcggt ggcgaaatga aaactatgca actctctaat ttacacggag ttcgaattgc
     2881 ttcggtgaat aattccaatg taatggaagc gagctgtctg gcaagtggaa tttatattct
     2941 ttctgtggtt tccacgaatg gaacaaaaga tactttcaaa ctgttaaaac cataagtttt
     3001 ataattatcc atattttttt tcagaaataa agaataatca aataaaaaag atgagaaact
     3061 taaaaacagc taaaatcggt tttttaaccg gatttatttt gctgacttct gcttttgtaa
     3121 acgctgaaga tatgctgagc ggcttccgct attccccaga acaagctcca aaaggcaacg
     3181 aatgggaatc tcccgaaaac ctcgcactca acaaagaaca gccgcgtgcc tggtttttct
     3241 cgtttcagga taccgaaagt gcccgcaaag tattgcccga aaactccgct tattggaaat
     3301 ccttagacgg aacgtggaaa ttcaactggg taaaacatcc cgacgaacgt cccgtggatt
     3361 tttacaaacc ggaattcaac gtaaatggat gggaaaatat tcctgtaccc gtaagctgga
     3421 atatccatgg
//
LOCUS       DAORKP010000196         3410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8081_length_3410_cov_7.757079, whole genome shotgun sequence.
ACCESSION   DAORKP010000196 DAORKP010000000
VERSION     DAORKP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_3410_cov_7.757079"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLN75_08790"
     CDS             complement(<1..269)
                     /locus_tag="PLN75_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04252.1"
                     /translation="MKKFIYIFLLIYPAIGNINAQIAPIVPSSTSIANASVAVQNDWS
                     AFQNTAALAHIDKLELAMQYENHSMVKEMSWKSAQAGINAKYVNVG"
     gene            476..1111
                     /locus_tag="PLN75_08795"
     CDS             476..1111
                     /locus_tag="PLN75_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRG04253.1"
                     /translation="MLIKISELRRYLKIFILLLITFSSAGVVAQDVTVAKDKFSYSTM
                     MEKVILNGDTLFVDYIREVYVFPPMVFTSKNQEKFYWRTVRDVKKALPYARMVSYEIT
                     QVNAELLKLPGDRERKQYISQYKKEAFRKYEKELRNLTVNQGKMLMKLIDREYEVNTY
                     ELVKGYLGAVPAFFWNSVAGLFGSTLRQDYDGKDKDRIVERVVILVDAGQL"
     gene            1174..1782
                     /locus_tag="PLN75_08800"
     CDS             1174..1782
                     /locus_tag="PLN75_08800"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRG04254.1"
                     /translation="MIGFSLKRFFEKNFSIFQKQKQIKDKKYQIRADIRAKKRQLSEE
                     EKKEAARIVFERIETMLAFQKAKVVFLYWSTPSELPTHEYVRKWSENKIILLPSVSGD
                     KMHLRRYVSSKEMQMGEGNILEPTTDIYKGHFDLAIIPGIAFDRERHRMGRGRGYYDR
                     FLNSKSITTYGVGFDFQVLRKIPVTRRDMKVTSVFSPSKTIE"
     gene            complement(1803..2516)
                     /locus_tag="PLN75_08805"
     CDS             complement(1803..2516)
                     /locus_tag="PLN75_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04255.1"
                     /translation="MPYRRLPNTDQARLRALRTAIQMGEKQSYDKQVVSFKAIHEAQT
                     FLNIFEKQMQLYKQTLESQVNANKQYQQILYNARMYISHFIQVFNLSVIRGEIKKEHK
                     LYYHLNPDTHQVPDLSTETAIQEWGKNIISGENERIRNGGTPVYNPTIAKVQVHFEVF
                     KEYKSTQRIYQSTTTHNWEELVNQRKKADAIILDIWNQVEEHYRNEKPYAKLMKCKKF
                     GLVYYYRRHEKELTPDDDL"
     gene            complement(2613..>3410)
                     /locus_tag="PLN75_08810"
     CDS             complement(2613..>3410)
                     /locus_tag="PLN75_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRG04256.1"
                     /translation="AMNSAIGLEIFHNFTLLHDDIMDRADMRRGKPTVHKKWNDNTAI
                     LSGDVMQIEAYKWMTQSPADKLKEILDLFSRTAAEICEGQQYDMEFENRENVTADEYI
                     EMIRLKTAVLIAAGCVIGARIGGAGAEDARKLYDFGINIGLAFQLKDDLLDVYGSEAN
                     FGKKIGGDILCNKKTYLLIHALKKAKGKDAEELNYWLKNINPESACEKVKAVTELYNR
                     LSVKDICEEAIDYYYTKAVASLAEVAVPDVKKSELRGFSEKLMSRKD"
BASE COUNT          951 a          674 c          729 g         1056 t
ORIGIN      
        1 ccaacattta catatttggc attgattcct gcctgagcac ttttccagct catttccttc
       61 accatcgaat gattttcata ctgcatggcc agttccagtt tgtcgatatg agccagtgcg
      121 gctgtattct gaaaagcact ccagtcgttc tgtacagcta cagatgcatt ggctatggag
      181 gtagacgagg gaacaatcgg cgctatctgt gcgttaatat taccgatagc aggatatata
      241 agcagaaaaa tgtagataaa ttttttcatc aattacattt atatgttgta aaacggcgat
      301 ttacagggct taactgaaat atcaattatt tatatcagga taaaagtaat tgttttttcc
      361 aaaacataga aataaattta aaaaatgttt attctaaaaa aaataatttg gaataaaaat
      421 tgttatatac ttgtggaact tgcaaataac gcttattttt gcaccacaat attgtatgct
      481 aataaaaatt agtgaattga gacgatatct gaaaatattt atactgctcc tgattacatt
      541 ctcctccgct ggagtcgttg cccaggatgt tactgtcgct aaggataagt tttcatattc
      601 cacaatgatg gaaaaagtaa tcctgaatgg agatacactt tttgtggact atatcagaga
      661 ggtatatgta tttccgccca tggtttttac cagcaaaaat caggaaaaat tctactggcg
      721 aacggttcgg gatgtaaaaa aggcacttcc gtatgcccgt atggtttcgt atgaaatcac
      781 ccaggtgaac gcagaattat tgaaattgcc gggtgacagg gagcgtaaac aatacatcag
      841 tcagtacaaa aaagaagcct tccgaaaata cgaaaaagaa ctgcgaaacc tgacggtaaa
      901 tcagggaaaa atgctgatga aactcatcga tcgcgaatac gaggtgaata cctacgaatt
      961 ggtaaagggt tatctgggtg ctgtacctgc ttttttctgg aattcggtgg ctggattatt
     1021 tggatctacg ctcaggcagg attatgacgg aaaggataaa gaccgtatag tggagcgtgt
     1081 ggtgatactg gtggatgccg gtcaacttta actgtaaatg ttcagaaata tttgtttacc
     1141 cgatataaac ctttgtttga aaaagatatt agtatgattg gattttccct taaacgattt
     1201 tttgaaaaga atttcagtat tttccaaaaa cagaaacaaa taaaagataa gaaatatcaa
     1261 attcgtgccg atatccgcgc caagaaacgg cagctatccg aagaggaaaa aaaggaagct
     1321 gcccgaattg tatttgaacg aattgaaacg atgcttgcct tccaaaaggc gaaagtcgta
     1381 tttctttact ggtctacccc ctcggaactt cccactcacg aatacgtaag aaaatggagc
     1441 gagaacaaaa ttattttgct cccctcggtt tcgggtgata aaatgcatct gcgccgatat
     1501 gtttcgtcca aagaaatgca gatgggcgaa ggtaatattc tggaacctac caccgatata
     1561 tacaagggac attttgatct ggccattatt ccgggcatcg cttttgaccg ggaacggcac
     1621 cgtatgggac gcggacgagg ctactacgat cgatttctca attcaaaaag tattacaacg
     1681 tatggtgtgg gatttgattt tcaggtatta cgaaagattc ctgttacccg tcgggatatg
     1741 aaagttacca gcgtgttcag tcccagcaaa acaatagagt aaacagccac tggctgaggg
     1801 atttacaggt catcatcggg tgtaagttcc ttttcgtgcc gtcggtaata atacaccaat
     1861 ccgaattttt tgcatttcat cagtttggca tacggttttt cgttccggta atgctcctct
     1921 acctgattcc aaatatccag aatgatggca tctgcttttt tccgctgatt gaccagttcc
     1981 tcccagttgt gagtggtggt gctttgatag atgcgttgag tggatttata ttccttgaaa
     2041 acctcgaaat gaacctgcac ttttgctatg gtggggttgt atacaggggt tccgccattc
     2101 ctgatgcgct cattttcacc gctgatgatg ttttttcccc attcctgaat ggccgtttct
     2161 gtgcttaaat ccggaacctg atgagtgtcg gggttcaggt gataatacag tttgtgctct
     2221 ttttttattt ccccccgtat gaccgaaaga ttgaaaacct ggatgaaatg tgagatatac
     2281 attcgggcat tgtataaaat ctgctgatac tgtttattgg cattgacctg actctcgaga
     2341 gtttgcttgt aaagctgcat ttgtttctcg aaaatattca aaaatgtctg agcttcatgg
     2401 atggctttga aggacactac ctgcttgtcg taactttgct tttcgcccat ttgtatggcg
     2461 gtgcgcaaag ctcttagtcg ggcctgatcg gtatttggca agcggcggta gggcatctgg
     2521 ttagttgaaa atagttaatt ccgggctgaa agttagttgt ttacaggggg ttatttcgca
     2581 ctaaataacg tttgtaagca aaagtacata atttaatctt tacgtgacat cagtttttct
     2641 gaaaatcctc ttaattcgga ttttttaacg tcgggtactg cgacctcagc gagcgacgct
     2701 actgctttgg tgtagtagta atcaatcgct tcttcgcaaa tatctttgac tgatagccgg
     2761 ttatagagtt ctgtcactgc tttaactttt tcgcaggcag attccggatt aatattcttt
     2821 aaccaataat tcagttcttc agcatccttt cctttggctt ttttcagggc atgaatgagt
     2881 aggtaagttt ttttattaca cagaatatcg cctccgattt tttttccgaa attggcttca
     2941 ctgccatata catcaagcag atcgtccttt agctgaaatg caagtccgat attgatgccg
     3001 aagtcataga gttttcgggc atcttctgca ccggcaccgc ctatacgggc acctatcacg
     3061 cagccggcgg caatcagcac agccgttttc agccggatca tttctatata ttcatcagcc
     3121 gtaacattct cacgattctc aaattccatg tcgtattgct gtccttcaca tatttcagca
     3181 gccgttctcg aaaaaagatc gagaatttcc ttcagtttgt cagccggtga ttgggtcatc
     3241 catttatagg cttcaatctg catcacatcg cccgaaagaa tagctgtgtt atcgttccat
     3301 tttttatgta ccgttggttt tccgcgtcgc atatcggcac gatccatgat atcgtcgtgc
     3361 agaagggtga aattgtgaaa tatctccagc ccgatggccg aattcatggc
//
LOCUS       DAORKP010000197         3387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8179_length_3387_cov_8.256002, whole genome shotgun sequence.
ACCESSION   DAORKP010000197 DAORKP010000000
VERSION     DAORKP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_3387_cov_8.256002"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1316
                     /locus_tag="PLN75_08815"
     CDS             42..1316
                     /locus_tag="PLN75_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG04257.1"
                     /translation="MIKNILLQQLEERNLLLSQAYISRIDLQTKTEYLSTTLIKLITG
                     PRRAGKSVLALQLLQNQNFAYLNFDDDLLLKHFNEDTVTLTLKEIYQNFEFLLLDEIQ
                     NLPNWELWVNKLYRRGLNLVITGSNSKLLSSEMATSLTGRYIQIPVFPFSFMEFLHVN
                     DPSIDVNPETPLETGKLLGWLHTYMLNGGFPETILNPGLLKNYLSSLFDSVLLKDILK
                     RFRIRQTQQLYDLSNFLLSNYTNLFTYNQIKENLGFNSVATVQKFVGYLEEPYLFQRI
                     TRYDTKIKQQQKASQKIFIIDNGFVQARSFELSPNYGRLLENLVFIELFRRNYKPELE
                     LFYYRTRNDREIDFLIRKGHRVEQLIQVCYDINQPKTLKRELDALTEAAAELKCNNLL
                     LLTWDREQLMEVNQFTVRLIPAYKWLISNRDL"
     gene            1993..3162
                     /locus_tag="PLN75_08820"
     CDS             1993..3162
                     /locus_tag="PLN75_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRG04258.1"
                     /translation="MESLFEKQQQLLARTNLKFKRYMFGKIPWESRMVGLTGPRGVGK
                     TTMLLQYIKEQNNHNILYVSADDLYFSNHRLTDLADEFYKNGGKHFFIDEVHKYKDWS
                     VELKNMYDFYPDLKICFTGSSILDIRKGMADLSRRALMFTMQGFSFREYLKFEHDIDV
                     PVHSFKQIISNQISLIDKLEHPLPVFRDYLQKGYYPVSAEDNMDIRLGQILNLTLEVD
                     IPQFANLSVTTARKLKKLLVIVAESVPFKPNFVSLAAKLEVSRNSLEEYLTYMEDAGL
                     ISRLRESASGIIGLGKVEKIYLDNPNLIHYLSQSKTETGNIRETFFLNQTRVNNPVTS
                     SAVADFKITDYTFEVGGRSKAQEQIKNTPNAFIVKDNIEYGYKNVVPLWAFGLNY"
BASE COUNT         1070 a          694 c          676 g          947 t
ORIGIN      
        1 tgaataaaaa tcactatttt tgttcattat aataccaact aatgataaaa aacatacttc
       61 ttcagcagct cgaagagcgg aatttattac tcagtcaggc ttatattagt cgtattgatt
      121 tacaaactaa aacagaatac cttagtacta cgctcatcaa acttattacc ggtccgcgca
      181 gggctggaaa atctgttttg gcattgcaac tgttgcagaa tcaaaacttt gcatatctca
      241 atttcgatga tgacctgctt ctgaagcatt tcaatgaaga tactgtcaca ctgacattaa
      301 aagaaatata ccaaaatttt gagtttcttc tgctggacga aattcaaaac ctgcccaact
      361 gggaactctg ggttaataag ttgtatcgca ggggactaaa tcttgtaatt accggatcga
      421 actcaaaatt gctatccagt gaaatggcaa catcgcttac aggacgatat attcagattc
      481 ctgtttttcc tttcagtttc atggagtttt tacacgttaa cgacccatcg attgatgtaa
      541 acccggaaac tccgttggag accggcaagt tactgggatg gttacataca tatatgctta
      601 atggtggatt tcctgaaaca attttaaatc ccggtttatt aaaaaattat ctttcatcgc
      661 tgtttgactc cgtgttactt aaagatatat tgaaacggtt ccggatacgc caaacccagc
      721 agctttacga tttgtcgaac tttttattat ctaactatac caatcttttc acttacaatc
      781 aaattaagga aaacctgggt tttaacagtg tggctactgt tcagaaattt gttggatatc
      841 tggaagaacc ctatttgttt caacgcatta caaggtacga taccaaaata aaacaacaac
      901 aaaaagcatc gcagaaaatc tttattattg ataacggatt tgttcaggca cgttcatttg
      961 aactttcacc caattacgga agactgcttg aaaaccttgt tttcattgaa ctttttcgcc
     1021 gcaattacaa acccgaactc gaattgtttt actatcgaac ccgcaacgat cgtgaaattg
     1081 actttctgat tcgtaaagga catcgtgttg agcagttaat acaggtttgt tatgatatca
     1141 atcagcccaa aaccctgaaa cgggagttag atgcattaac cgaagcagca gcagaattaa
     1201 aatgcaacaa tttattactt ctaacctggg atagagagca gttaatggag gtaaatcagt
     1261 ttacagttcg actcatacct gcatacaaat ggctaatttc aaatcgtgat ttatagcatg
     1321 ttgtaaatca aattcataca accggtaaat gacgaaaaga cggcagacag cggacaacgg
     1381 acgccagacg atgcgaaaat aacttcaaaa cgtcgtaagt tccgatagct atcggaatga
     1441 acgtttctta ggaagataaa gcccccctgc cccccagccc ctatccccta cgggacttcc
     1501 cccacaaggg gaaggaagtg gattttttag tttatggggt tcaaaacagg gttaagtaaa
     1561 cagcatttag tgagcgggtg actccgagtc acccgctcac tattgcgcaa ttgaatcaga
     1621 ttccctgcca gaggcaggca agctcacttc gcttcggcct gcccgcctct ggcgggaatg
     1681 acaaggccgc ttttttcaat tcccgtcgcc accggcacag aaacgatgaa aatgaaagat
     1741 cgacgggtgc gaggctttaa gttccccctt cgggggattt cccgataaat cgggacgcgg
     1801 tagtaggcca ctaaagatga aatcacgaag ggtgcggaaa gaatcgtaaa ttgtaaattg
     1861 taaacacatt acgtcaacgc gtcacccact cactatccga tttaaactgc aaaaatattg
     1921 ataaaatgca caacaaaatg aacattaatg gaaaattatg ctaattttgc agtgcaataa
     1981 taacaattta ttatggaatc gctttttgaa aaacagcaac aactgcttgc ccgcactaac
     2041 ctgaagttca agcggtatat gttcggcaaa attccatggg aaagtcgcat ggtggggctt
     2101 accggtccac gcggtgtggg aaaaaccacg atgctgctac aatatatcaa agaacaaaac
     2161 aaccacaata tattgtatgt gtcggctgac gatttatatt tttcaaatca cagattgaca
     2221 gatttggcag acgaatttta caaaaatggc ggtaaacatt tttttatcga cgaagttcat
     2281 aagtacaaag actggtcggt ggagttgaaa aacatgtatg atttttatcc ggacttgaaa
     2341 atatgtttta ccggctcttc tattctcgat attcgtaaag gaatggctga tttgagtcgt
     2401 cgcgcgctga tgtttacgat gcagggtttt tcgtttcgtg aatacctgaa atttgaacat
     2461 gatatcgacg taccggttca ttcgttcaaa cagattatca gtaatcagat ttctctgatt
     2521 gacaaattgg aacacccgct gccggttttt cgcgattatt tgcaaaaagg ctattatcct
     2581 gtttctgcag aagacaacat ggatatacgg ctcggacaga ttttgaacct gacactcgaa
     2641 gtggatatac cgcagtttgc taatttatca gttacaacag cacgaaaact gaaaaaactg
     2701 ttggtcatcg tggctgaaag cgtaccattt aaaccaaatt ttgtttcatt ggctgcaaag
     2761 ctggaggtga gccgtaattc attggaagaa tacctcacgt acatggaaga tgcaggactt
     2821 atttcgcggt tacgcgaatc ggcatcaggc ataatcggac tgggcaaggt agaaaaaata
     2881 tatttagaca accccaatct gattcattat ttatcacaaa gtaaaactga aaccggaaat
     2941 atccgcgaga catttttcct gaatcaaaca cgggttaata atccggttac ttcttcagct
     3001 gttgccgatt tcaaaataac tgactatacg tttgaagtgg gaggtagaag caaagcgcag
     3061 gaacaaatca aaaacacgcc caatgccttt attgtaaaag ataatattga atacgggtat
     3121 aaaaacgtgg tgccattatg ggcttttgga ttgaactatt gactcaatct tagcacacac
     3181 tccatcgttt cctaacagcc tgtcccgacc cgatcgctat cgggacggga aggggtaagt
     3241 ggttgagcgt ttgtaaccca aaaaaacata attagattcc tcactgcgtt cggaatgaca
     3301 agttcacttt tttcaattcc cgtcgcccac cggcactgat ataatgagat gaaatatcga
     3361 cgggtgcaaa gttattcaat cccccta
//
LOCUS       DAORKP010000198         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8368_length_3344_cov_7.532989, whole genome shotgun sequence.
ACCESSION   DAORKP010000198 DAORKP010000000
VERSION     DAORKP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8368_length_3344_cov_7.532989"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLN75_08825"
     CDS             complement(<1..581)
                     /locus_tag="PLN75_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRG04259.1"
                     /translation="MKTTPFTDFHIALGAKMHEFAGYNMPIEYSGINDEHLTVRNGVG
                     VFDVSHMGEIWVKGPKALDLIQQVCSNDASKLSIGQAQYTCFPNGKGGIVDDIIVYYF
                     EPEKYMLVVNASNIEKDWNWLNAHNSVGAELENASEHTAQLAVQGPKATELLQKLTPV
                     NLSEVPYYHFTVGEFAGVKNVILSNTGYTGSGG"
     gene            complement(608..1843)
                     /gene="pepT"
                     /locus_tag="PLN75_08830"
     CDS             complement(608..1843)
                     /gene="pepT"
                     /locus_tag="PLN75_08830"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRG04260.1"
                     /translation="MNITDRFLKYVSFTTTSDENTNLTPSTPGQMVFAKYLADELKSI
                     GLKDVTLDKNGYIMATLPANSGKKDVPVIGFVSHMDTSPDASGKNVKPKIIEHYDGND
                     ILLNEEKVIVLETEKYPEILQYKGQDIIVTDGTTLLGADDKAGIAEIVSAMEYLIEHP
                     EIKHGKIRVGFTPDEEIGQGADHFDVKEFGAEWAYTMDGSQIGELEFENFNAAAAKVI
                     IHGLNVHPGYAKHKMKNSMRIATQFVTMLPRHETPEHTEGYEGFYHLTNVEGTVEKTT
                     LSYIIRDHDRDRFERRKKEVQHLANKINAEFGENTAVVELRDQYYNMREKVEPVMHIV
                     DLAFEAMKENGIEPIVKPIRGGTDGARLSFEGLPCPNIFAGGHNFHGRFEYVPVQSME
                     KAKMVIVKIAELLEKKTEN"
     gene            2165..3244
                     /locus_tag="PLN75_08835"
     CDS             2165..3244
                     /locus_tag="PLN75_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRG04261.1"
                     /translation="MSIYSVITGTGSCIPEKVVKNEDFLQNEFYDNSGEKIATPTSQI
                     IEKFREITTIESRRHVTDEQSTSDLAKIAAERAIESSGIDREELDYVIVAHNFGETHA
                     DNKRVSVLPIVSALLKQKLGIRNPGAVAYDILFGCPGWIQALIQADYYIKSGDAKKVL
                     VVGADILSRISDPHDRDSMIYADGAAAVVVEGVESDVPVGIIGHNSRSDSLEFSQMLS
                     MGYSYNHQEAEKKEYYLKMNGRRLYQYALEFVPETIKAGLDKLNLHIKDIEKVLLHQA
                     NGKMDDAIIKRLYKLYNIKKVPEDVMPMTISWLGNSSVATVPTLFDLLIRGELDTKLP
                     EKDDLLVFASVGAGMSINSIVYKMP"
BASE COUNT          923 a          646 c          715 g         1060 t
ORIGIN      
        1 aaaccgcctg aaccggtata gcctgtgttt gagagaatta cgtttttgac acctgcaaat
       61 tcacctacgg taaaatggta gtaaggaact tccgacaaat taaccggagt taatttttga
      121 agtaattcgg tggctttcgg accctgaacg gcaagctgcg ccgtatgttc agaagcattt
      181 tccagttcag cgccaacgga attgtgtgca ttcagccagt tccagtcttt ttcaatgttt
      241 gaagcgttga ctaccagcat atatttttcg ggttcaaaat agtaaacgat gatgtcgtcc
      301 acgattccgc ctttaccgtt cgggaagcag gtgtactgag cctgaccgat agaaagtttt
      361 gaggcatcgt tggagcaaac ttgctgaatt aaatccagcg cttttggtcc tttcacccaa
      421 atttcgccca tatgcgaaac gtcgaagacg cctacgccgt ttcttacggt caagtgttcg
      481 tcattaattc cactatactc gatgggcatg ttgtaacctg caaattcatg catttttgca
      541 cctaaagcga tatgaaaatc tgtaaatgga gtagttttca tgtgattttt taaaaataat
      601 gttatgatta gttttcggtt ttcttttcga gcaattcagc aatttttaca ataaccattt
      661 tggctttttc catggattga acgggcacgt attcaaatcg tccgtggaaa ttgtgtccgc
      721 cggcaaaaat gtttgggcag ggcagtcctt cgaatgaaag gcgggcgccg tcggttcctc
      781 cgcgaatggg tttcacgata ggctcaatac cgttttcttt catcgcttcg aaagccagat
      841 ccacgatgtg catcaccggt tccacttttt cgcgcatgtt gtagtactgg tcgcgcagct
      901 ccacaacggc ggtattttca ccaaattcag cattgatttt gttggctaaa tgttgcactt
      961 cctttttgcg tctttcaaaa cggtcgcggt cgtgatcgcg gataatgtag ctcaacgtcg
     1021 ttttttccac ggttccttcc acgttggtca ggtggtaaaa accttcgtaa ccttcggtat
     1081 gttcgggagt ttcgtgtcgg gggagcatgg ttacaaattg cgtggcaatt cgcatggaat
     1141 ttttcatttt atgttttgca taccccgggt gaacgttcaa tccgtggata attacttttg
     1201 cggcagctgc gttgaagttt tcgaattcaa gttctcctat ctggcttcca tccatcgtgt
     1261 aagcccattc agcaccaaat tctttcacat caaagtggtc ggcaccctgt ccgatttctt
     1321 cgtcgggagt gaagccaaca cggattttgc cgtgttttat ttcaggatgc tcaatcaggt
     1381 attccattgc actcacaatt tcggctatac cggctttatc atcagctcca agcagcgtgg
     1441 ttccgtcggt tacaattatg tcctgtcctt tgtactgaag aatttccggg tatttttctg
     1501 tttccagcac aattactttt tcctcattga gcaggatgtc gttaccatcg taatgttcta
     1561 tgattttggg ttttacgttt ttgccgcttg catccggact ggtgtccatg tgtgatacga
     1621 aaccgataac aggaacatct tttttgccgg aattggcagg taaagtagcc ataatgtatc
     1681 cgtttttatc taacgtaacg tctttcaatc caatcgattt cagttcgtca gcaaggtatt
     1741 tggcaaatac catttgcccg ggggtactgg gtgtcaggtt tgtgttttca tcggaagttg
     1801 tcgtaaaact tacatacttc aaaaatcggt ctgtgatgtt catttttctt gtttttatat
     1861 tttgactaca aatttaacta tttaatcttt atttatgttc attttattcc tgatttaaaa
     1921 accttttgga cgaatttttt cccgaatatg tcatttttta taaatgatga taaaaaaatg
     1981 catacaatga tgtaatgacg acgaatgatt gattgtaaaa aagagtatga tttccaatca
     2041 tgaaattaac ataaaatgag cggaaaggtt aaatattata gtagaatatg tttaaaaaca
     2101 gttttttgaa atttattttc taattttgca ccggatttaa attgataaat aaattttttt
     2161 atatatgtca atctatagtg taataacagg aacaggaagc tgtattcctg agaaggtggt
     2221 gaaaaatgaa gattttcttc agaatgaatt ttatgataat tcaggtgaaa aaatcgctac
     2281 accaacttcg cagattattg aaaaattcag ggaaataacc accatcgagt cgcgcaggca
     2341 cgtcaccgac gaacagagta cttccgattt ggcaaaaatt gctgccgaac gtgctattga
     2401 atcatcagga attgacagag aagaactcga ttatgtgata gttgcgcata attttggtga
     2461 aacgcatgcc gacaacaaac gtgtcagcgt acttccgatt gtttcagctt tactaaagca
     2521 aaaactgggc attcgtaatc cgggtgctgt ggcttatgat atactttttg gctgtccggg
     2581 ctggatacag gcacttattc aggcagatta ttacatcaaa tccggtgatg caaaaaaagt
     2641 actggtggtt ggtgcagata ttttatcacg catttctgat ccgcacgacc gcgacagtat
     2701 gatttatgcc gacggagctg ctgctgttgt agtggaaggt gtggaaagtg atgttccggt
     2761 aggaatcatc ggacacaact cacgtagcga tagtttggaa ttttcgcaaa tgctcagcat
     2821 gggatactcc tataatcatc aggaagccga aaagaaagag tattacctga aaatgaacgg
     2881 acgaaggtta tatcagtatg ccctggagtt cgtgcccgaa acaatcaagg ccggtttgga
     2941 caaactgaat ctacacatca aagatattga aaaagtgctt cttcatcagg caaacggaaa
     3001 gatggacgat gcgataatca aaagacttta caaactgtat aatattaaaa aagtaccgga
     3061 agatgtaatg ccgatgacta tctcctggtt aggcaattca tcggtggcaa cagtgccaac
     3121 acttttcgac ttattgattc ggggcgaact ggacactaaa ttgcccgaaa aagatgattt
     3181 attggttttt gcatcagtag gagccggtat gagcatcaac agtattgtgt ataaaatgcc
     3241 ataacaagct gatagttact atatttgctt ataaaaaaag gtaaacctca atgcaggctt
     3301 accttttttt ttagtaataa atatttttca atcagttggt tatg
//
LOCUS       DAORKP010000199         3341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8377_length_3341_cov_9.755021, whole genome shotgun sequence.
ACCESSION   DAORKP010000199 DAORKP010000000
VERSION     DAORKP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8377_length_3341_cov_9.755021"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLN75_08840"
     CDS             <1..420
                     /locus_tag="PLN75_08840"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRG04262.1"
                     /translation="RLTKDEQYTLMNLFTIFKSPLMFGGDLPSNDAFTLSLLTNKNVL
                     KMHKEGTEVRQLFQQDKKLAITSRNRKTGEVYLALFNLSDENNKRKVEVNLSEIGMQE
                     CSATDLWSGKKTGFYSGKISAELPPHASLLLKLKKTK"
     gene            442..2241
                     /locus_tag="PLN75_08845"
     CDS             442..2241
                     /locus_tag="PLN75_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRG04263.1"
                     /translation="MKRLFFLSIGIFSIISLAAGETVLQNVFSRNTQSLNGKWHYIID
                     PFDNGYFDYRLRVNPNGFGKNYQAKNKSELVEYNFATSPLMQVPGDWNTQREQLFFYE
                     GSIWFQRDFDYTPRPGTKVFLYFGAVNYLCNVYVNGQNVGMHEGGFTPFNFDVTDKLK
                     NGNNFVVLRVNNNRKPEGVPTINADWWNYGGITREVMLVEVPETFIDDYSVRLAKGSY
                     QEIEVSVKLNSAVEGAPVDVNIPELKFSKTITTDKNGSSSFTFKAKPQLWSPENPKLY
                     AVRISQNSEVLVDKIGFRQIETHNKDILLNGKKVFLRGISIHEEAPFRQGRAWSKEDA
                     AVLLGWAKELGCNFVRLAHYPHNENMIRLAEEMGFMVWSEIPVYWTIHWDNPDTYLNA
                     ANQLTDMIDRDHNRCAVVIWSIANETPHSDDRKDFLAKLARLARYKDESRLISMAMEV
                     TGESATLSKVNDPMNEYVDIVSFNQYLGWYGGNLESPKTRNFEIPYNKPVFISEFGGG
                     ALQGMHGDKTERFTEEYQEELYKNTLEMLDRIDGFSGTSPWILVDFHSPRRQLTGVQD
                     FFNRKGLISNDGIKKKAFWTLKNFYENKKEQYK"
     gene            2254..>3341
                     /locus_tag="PLN75_08850"
     CDS             2254..>3341
                     /locus_tag="PLN75_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HRG04264.1"
                     /translation="MMRICISLALSLIFLLQLSAQSTLNSKPDSVYLLSYATTKNRGH
                     NGLHYAWSHDGKSWNKVSNEFSFVKSDYGSWGSQKRMLSPFVIFGKDKKWHAVWSLNE
                     DESRFAHAESDDLISWRRQSYPDIKEGNFLDPIVSFNPNSNNYSVLFKSGDQLKKVTT
                     SDFKTYTEETLIAQSDYKDPRKSFEIDGENVTGNVHRVSWWTLEKILNHAELKNYKNL
                     LYSETTSQDAVRFAGLKPVDVTIKAIPENSKEISDMLIGIFYEDINYAADGGLYAELV
                     QNRSFEYTPSDRNNRDQSWNHSHSWSLSDQSDLKVETASPIHENNPTYAVLDLKNPGV
                     ALSNVGFAGIVIRQGEKYDFSVFTKQISG"
BASE COUNT         1035 a          671 c          785 g          850 t
ORIGIN      
        1 cgactgacga aagatgagca gtacacactg atgaatctgt ttaccatttt caaatctccg
       61 ctgatgtttg gaggcgatct gccaagcaac gacgctttta cactatcctt gttgaccaat
      121 aaaaatgtgt tgaaaatgca caaagaagga actgaggttc gtcagctttt ccaacaggat
      181 aaaaaactgg ccatcacttc cagaaacaga aaaacaggcg aggtttacct ggcactgttt
      241 aatctttcgg atgaaaataa caagcggaaa gtggaggtaa atctgagcga aattggtatg
      301 caggaatgct ctgcaaccga tttgtggagc ggtaagaaaa ccggatttta ctcgggtaaa
      361 atcagcgctg aattgccacc gcacgcatct ttactgctga aactgaaaaa gacgaaataa
      421 ccatttaaat aattgattgc aatgaaaaga ctattttttt tgtcgattgg catattctcg
      481 attatatcat tggcagccgg tgaaaccgtg ttgcagaatg ttttttcaag aaatacgcag
      541 agtctgaacg gaaaatggca ctacatcatc gatccgtttg acaacggata tttcgactac
      601 cggctgcgtg tcaatccgaa cggattcggg aaaaactatc aggcaaaaaa caaatccgaa
      661 ctggtggaat acaactttgc cacctcaccg ctgatgcagg tgcccggcga ctggaataca
      721 cagcgggaac aattattttt ctacgaagga agtatctggt ttcagcgcga ttttgactac
      781 actccccgac cgggaacgaa agtgttttta tacttcggtg ccgtgaatta tttgtgcaac
      841 gtgtatgtga acggtcagaa tgtgggtatg cacgaaggtg gattcactcc ttttaatttt
      901 gatgtgaccg acaaactgaa aaacggaaac aactttgtgg tgctccgtgt aaataacaac
      961 cgcaaacctg aaggagtgcc tacgatcaat gccgactggt ggaattacgg cggaattacc
     1021 cgcgaagtga tgctcgtgga agtgcccgaa actttcattg atgattattc ggtccggtta
     1081 gccaaaggca gctaccagga aatagaagtg agcgttaagc tgaattcagc cgtcgaggga
     1141 gcgccggttg acgtgaacat tccggaactg aaattcagta aaaccattac caccgataag
     1201 aatggaagtt catcatttac attcaaagcc aaacctcagc tttggtcgcc ggagaatccg
     1261 aaactttatg ccgtgaggat atcacagaat tcggaagtac tggtagataa aatcggattc
     1321 aggcagattg aaactcacaa caaagacatt ttgctgaacg gaaagaaagt ttttctgcgt
     1381 ggaatcagca tccatgaaga agcccctttc cggcagggaa gagcctggag caaagaagat
     1441 gctgctgttt tgttgggctg ggccaaagaa ctcggttgta actttgttcg tctggctcat
     1501 tatccgcaca acgaaaacat gatccgtctg gccgaagaaa tgggtttcat ggtttggtcg
     1561 gaaattccgg tttactggac catccactgg gataatcccg atacttatct aaacgctgcc
     1621 aatcagctca cagatatgat tgatcgtgat cataaccggt gtgcggtggt gatatggtca
     1681 atagccaatg aaacaccaca cagcgatgac cgtaaagatt ttctggctaa actggccagg
     1741 ctggccaggt acaaagatga atcacgcctc ataagtatgg ccatggaagt gacaggcgaa
     1801 tcggctacgt tgagtaaagt gaatgatccg atgaacgaat acgtggatat cgtaagtttc
     1861 aaccagtatc tgggctggta cggaggaaat ctggagtcgc caaaaacgag aaattttgaa
     1921 attccgtaca ataaaccggt gtttatcagc gaatttggtg gtggtgcact acagggtatg
     1981 cacggtgata aaaccgagcg tttcaccgaa gaatatcagg aagagttata taaaaacacc
     2041 ctggaaatgc tcgatagaat tgatggattc tcgggcactt cgccctggat tcttgttgat
     2101 tttcactcac ccagacgaca attaacaggc gttcaggatt ttttcaaccg aaaaggactg
     2161 atttccaacg atgggattaa gaaaaaggcg ttctggacgc tgaaaaattt ttatgaaaat
     2221 aaaaaagaac aatataaata aaagggtgac aaaatgatga gaatctgtat tagcctggcg
     2281 ttatcgctaa tttttttatt gcagctttca gctcaaagca ccttaaacag caagccggat
     2341 tcggtttatc ttttgtcgta cgcaaccacc aaaaaccgcg gtcacaacgg cctgcattac
     2401 gcttggagcc acgatggaaa atcctggaac aaagtgagca acgaatttag ttttgtaaaa
     2461 agcgattacg gctcctgggg ctctcagaaa aggatgttgt ctccgtttgt aatttttgga
     2521 aaagacaaaa aatggcacgc agtgtggtcg ctgaatgaag atgaaagccg ttttgcacat
     2581 gccgaatcgg atgatttgat aagttggagg cgccagagct atcccgatat caaggaagga
     2641 aattttttgg atccgatcgt tagttttaat cccaattcaa acaattattc ggttcttttc
     2701 aaaagtggag atcagcttaa gaaagttact acttccgatt ttaaaaccta caccgaagaa
     2761 actttaattg ctcaatccga ttacaaagat ccgcgaaaat cttttgaaat tgacggtgaa
     2821 aatgtaacgg gaaacgtaca cagggtttcc tggtggacgc tggaaaaaat tttaaatcac
     2881 gccgaactca aaaactacaa aaacctcctt tacagcgaaa cgaccagtca ggatgcagtc
     2941 cgttttgcag gattaaaacc tgtggatgtg accataaaag ccattcctga aaactcaaaa
     3001 gagatttcgg acatgctgat cgggattttt tacgaagata ttaattatgc tgctgacggc
     3061 gggctctatg ccgaactggt gcaaaaccgg agttttgaat acacgccttc cgacaggaac
     3121 aaccgcgatc agtcgtggaa tcacagccat tcgtggagcc tttcggatca aagcgatttg
     3181 aaggttgaaa ctgcttcccc cattcatgaa aataatccaa cctacgcagt tttggatctg
     3241 aaaaatccgg gtgttgcgtt gtcgaatgtt ggttttgcag gaattgtgat caggcaggga
     3301 gaaaagtacg atttttcggt gtttaccaaa caaatttcag g
//
LOCUS       DAORKP010000200         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8457_length_3324_cov_7.862343, whole genome shotgun sequence.
ACCESSION   DAORKP010000200 DAORKP010000000
VERSION     DAORKP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8457_length_3324_cov_7.862343"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1049)
                     /locus_tag="PLN75_08855"
     CDS             complement(81..1049)
                     /locus_tag="PLN75_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04265.1"
                     /translation="MRRLILIAGLMLFLSGITSDLTAQRNSNIGYYTIESECLGSELD
                     GSITLRAWGTGRNRFDAVDQAKKNALREVIFKGIRKGSPECNQRPLLPEVNAEMKYED
                     FFNRFFSDRTDDYKKFCSGKDERLDNKIFRRGMGDSKMVTYSVIVRVLRAELKDYLAG
                     GFSPQREPAEINLPSIGIVREEKINWEYEIEPVSVGTQGTYLIKVWSYSRNPELAIEQ
                     AKRNAVHGVIFKGFGGISGVPGQHPLADSPNLEVEQAEFFRNFFANGGKYMKFVNITN
                     DGSVAAEDRLKIGKEYKIGVVVSVNVAGLRRDLEEAGIIRSLGSGT"
     gene            complement(1120..>1617)
                     /locus_tag="PLN75_08860"
     CDS             complement(1120..>1617)
                     /locus_tag="PLN75_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04266.1"
                     /translation="TCLLLIVLAGFAPETTAQKRKADKDTQNWIYEIEPVAVGSQGSY
                     LIKVWSYSKKPVIAIEQAKKNAVHGIIFKGFTGIQGVPGQRPLTSNPNLEVEQAEFFK
                     NFFADGGKYMKFVNITNDGAVAAEDRIKMDKQYKIGVVVSVNVAGLRKDLEDAGMVRG
                     LNSGF"
     assembly_gap    1618..1717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1718..3144)
                     /locus_tag="PLN75_08865"
     CDS             complement(<1718..3144)
                     /locus_tag="PLN75_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04267.1"
                     /translation="MKRYLILLTLVSFFTGLAAQNTNGKINTRYLRPSITMLFAEPKD
                     ADEAILINKFRNLEVPGKFDDHRIQFPDMKPVENTNPAKKTLLENYIRKASNPVIAKW
                     WGRDEQGNFNYTLVAKRASYTATDADAIISRGSNTSRIEMLGEQLIDRSYILLYEITD
                     LYTMDEYYDRLDAQNRKKKDYTPAKRTEEGYNCDYKVYAYKVAFNDSVAAEFYNRYWV
                     DAQNPDKQKAAAWADATFPVRLITEAAGSVRSSQTKAKEKNKVKKTMQELLEDIPANI
                     QSGAAFELGRKIEDFKLKVTVFKAYPVTAKLGTKEDLYTDQRFYVYEIEQDRSGNQST
                     VRKGVVRAKTINDNKGIATGTSLPSAFQQVQGKRIYPGMFMESKDDYGIILNTGFVSS
                     GNKAMGGFHIGADIRISRFIKKPGWHFGIDVAINPFKNVYAGDIETDTRTLISSADVC
                     SGNTLALAVTGSKEMYFTRRGNIYLR"
BASE COUNT          680 a          771 c          788 g          985 t
ORIGIN      
        1 ctcgcatgcc aattttcatc tgaattggtg aatgcttgcg ttcatgctcg tttcatgttg
       61 atttttttta aataaaataa ttaagttccg ctgcccagag agcgtataat gcctgcttct
      121 tcgaggtccc tgcgcaatcc ggctacattg actgatacaa caacgccgat tttgtattct
      181 tttccgattt tcaacctatc ttcggctgct accgagccat cgttggtgat gttgacaaat
      241 ttcatgtatt taccgccgtt ggcaaagaaa ttccggaaga attcggcttg ctccacttcc
      301 aggttcggac tgtcggcgag cggatgctgc ccgggtacgc cgctgattcc gccaaagccc
      361 ttgaatatca caccgtgtac ggcgttgcgt tttgcctgtt cgatagccag ttcgggattg
      421 cgggagtacg accacacttt gatgaggtaa gtgccctgag tacctacact caccggttcg
      481 atttcgtatt cccagttgat tttttcttct ctcacaatgc ctatcgaagg caggttgatt
      541 tcggcgggct cacgctgcgg actgaatccg cctgccaggt agtctttcag ttcggcccga
      601 agcacgcgga caatgacgct gtaggtcacc atcttggagt cgcccatgcc gcgccggaat
      661 atcttgttgt cgagccgctc gtctttaccc gagcagaatt ttttgtaatc gtcagtccga
      721 tcggagaaga aacggttgaa aaaatcttcg tatttcattt cggcgtttac ttcgggcagc
      781 agcggacgct gattgcattc cgggctgcct ttgcgtattc ctttgaatat cacttctctg
      841 agggcattct ttttggcctg atccacggca tcaaaccggt ttcggcccgt tccccaggct
      901 cgcagcgtaa tggagccatc cagttcggaa cccaggcatt cggattcgat ggtgtagtag
      961 ccaatgttgg aattgcgctg agctgtcaga tcggaagtta ttcctgacag gaacaacatc
     1021 agccctgcta ttaatattag tcgtctcata gcattcagtt tgtgaggttt ggtaagttta
     1081 tcaggttttt gaaagcttgt gctgattgct cacaatttat taaaatccgc tgttcagacc
     1141 gcgcaccatt ccggcgtctt cgaggtcctt gcgcagtccg gccacgttga ccgataccac
     1201 cactccaatc ttgtactgct tgtccatttt tatccggtct tcggctgcta cggctccatc
     1261 gttggtgatg ttcacgaatt tcatatactt tccgccgtcg gcaaagaaat ttttgaagaa
     1321 ttcggcctgt tccacttcca ggttcggatt actggtcagc ggtcgttgtc ccggcactcc
     1381 ctgtatgccg gtgaaccctt tgaaaataat gccgtgtact gcatttttct tggcttgctc
     1441 gatggctatg accggtttct tcgaatacga ccagactttt atcaggtaag agccctgact
     1501 ccccacggct accggttcga tttcgtaaat ccagttttgg gtgtccttgt cggcttttcg
     1561 cttttgagct gtcgtttcgg gtgcgaatcc tgccagtacg atgaggagca ggcaggtnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc aggtagatat tgccccgacg
     1741 ggtgaaatac atttcttttg aaccggttac tgccagtgcc aatgtgtttc ccgaacacac
     1801 gtcggccgat gaaatgagtg ttcgtgtgtc ggtttcgata tcgcctgcat atacattctt
     1861 aaacggattt atcgcaacgt cgatgccgaa atgccagccc ggttttttta taaatcgtga
     1921 aatccggata tctgcaccga tgtgaaatcc gcccatggct ttgttgcccg aacttacgaa
     1981 tcccgtattc agtatgattc cgtagtcgtc cttgctttcc ataaacatac ccggataaat
     2041 ccgtttcccc tgaacctgct gaaatgccga tggcaatgaa gtgccggtgg caatgccttt
     2101 gttatcgttg atggttttgg ctcttaccac cccttttctt accgtgcttt gattgccgct
     2161 gcggtcctgt tcaatttcat acacgtagaa acgctggtcg gtgtataaat cttctttggt
     2221 gcccagtttg gcggttacgg ggtaggcttt gaataccgtt actttcagtt taaaatcctc
     2281 tattttccgt cccagttcga atgcagcacc cgactgtatg tttgccggaa tgtcttcgag
     2341 caattcctgc atggtttttt tcaccttgtt tttttctttc gctttggttt gcgaggagcg
     2401 caccgagcct gcagcttcgg taatgaggcg taccgggaaa gtggcatcag cccaggcagc
     2461 agctttctgc ttatcgggat tttgggcgtc cacccaatac ctgttgtaga actcggcagc
     2521 taccgaatcg ttgaaggcaa ccttgtaggc atacaccttg taatcgcagt tgtagccctc
     2581 ttcggtacgt tttgcgggcg tgtaatcttt cttttttctg ttttgtgcat ccagccggtc
     2641 gtagtattca tccatggtgt agaggtcggt tatttcatac agcaggatat acgagcgatc
     2701 gatgagctgc tcgccaagca tctctatgcg gctggtgttg gatccgcggg aaatgatggc
     2761 atcagcatcg gtagccgtgt aagaagccct cttagctacc agcgtatagt tgaaattgcc
     2821 ctgttcgtcg cgtccccacc acttggctat caccggattg gaagcttttc ggatgtagtt
     2881 ttcgagaagt gtctttttgg ccggattggt attctccacc ggtttcatat ccggaaactg
     2941 tatccggtgg tcgtcgaact tgccgggcac ttcaagattt cggaatttgt tgatgagaat
     3001 tgcctcatcg gcatctttcg gttctgcaaa gagcatagtg atggaggggc gcaggtacct
     3061 ggtgtttatt tttccgttgg tgttttgcgc cgcaaggcca gtgaagaagg aaactaacgt
     3121 aagaaggatt aaatagcgtt tcatgtatct caaattttga tttgcagctc tgtgaaagca
     3181 acaaaaggtt aatactggat tttggaatca agtgcgggga aggggagggt cagccctgga
     3241 aaatgacctg tattaatttt tgaaaaaagt aaaaattata cggtaaattc aattgatgat
     3301 gcagaaatta atcattatac ttcg
//
LOCUS       DAORKP010000201         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_8836_length_3249_cov_7.428929, whole genome shotgun sequence.
ACCESSION   DAORKP010000201 DAORKP010000000
VERSION     DAORKP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8836_length_3249_cov_7.428929"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1152)
                     /locus_tag="PLN75_08870"
     CDS             complement(160..1152)
                     /locus_tag="PLN75_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRG04268.1"
                     /translation="MKKVLSMGNALTDILFQINNDEILSGLNLKKGSMQLVDAEKSDE
                     ITALFNDFPTTMATGGSAANTIKGIAKLGLEGGFIGKVCDDNIGSFFREDSRKNGIKP
                     HLKFSNTASGHCTVLISPDGERTLCTYLGAASELSAGDLESSVFTGYDIFHIEGYLVQ
                     DHNLIRTALQLAKKAGLTVSLDLASFNVVEENREFLHEIIRDYVDITFANEEEAKAFT
                     GKNPFEALTDIAHLCDIAIVKLGKDGSLIKSGEKTDEVIPSGDIRVDSTGAGDMYAAG
                     FLYGLARGYALNICGMIGSLISGRVVQVVGTTMSEETWNEIYAEIDDILKMETK"
     gene            1246..1319
                     /locus_tag="PLN75_08875"
     tRNA            1246..1319
                     /locus_tag="PLN75_08875"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1280..1282,aa:Asn,seq:gtt)
     gene            complement(1466..>3249)
                     /gene="pheT"
                     /locus_tag="PLN75_08880"
     CDS             complement(1466..>3249)
                     /gene="pheT"
                     /locus_tag="PLN75_08880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRG04269.1"
                     /translation="RYSALTISGVTVAESPQWLKESLMTIGLRPINNVVDITNFILHE
                     LGQPLHAFDADKITSGKVCVKNLPEGTPFTTLDGVERKLSSEDLMICNGDEPMCIGGV
                     FGGLSSGVTETTKNIFLESAYFHPVNIRKTARRHGLNTDASFRFERGCDPDITIYALK
                     RAAMLIQEVAGGTISSEIVDEYPVIVQPFEVALNKEKIDNLIGKKIGNDTIVKILNGL
                     EMEIVSEDAEKFQLKVPAYRVDVQRDVDVIEDILRIYGYNNVEIGDKLISTLSYAAKP
                     DSHKMQILISEQLTAQGFNEVLNNSLTKGGYYSTLKDYPETQSVKIINPLSSDLNVMR
                     QTLLFGGLENISRNINYRNADLKFYEFGNCYHFNADNKSADDHLKAYSEEYHLGLWVT
                     GNKFSDSWSGGTQKSSFFELKAYVQNIFLRMGFDFRSLKSEEYADDLFSEALAIRSAR
                     GVLLATYGIVNHQVRKMTDVDAEVYYADLLWNNILSELNISAVKYTELPKFPVVRRDL
                     AFLLDKNTTFAEIEKIAFETERKLLKKVSLFDVYEGKNLETGKKSYAVSFMLQDETKT
                     MTDNVIDSIMRKIQQNIENKLNAKLRS"
BASE COUNT          881 a          713 c          666 g          989 t
ORIGIN      
        1 aaataatgta tgaaactccg atcatgcgcc attttacagt agaactggct cccgtgaagt
       61 aaaatcaaat catacagaaa aacagaataa acaaatcgga aaggaatgat ggtatgtcat
      121 tcctttccga ttattaatgt aaaaaataca gttttaagat tactttgttt ccattttcag
      181 aatgtcatca atttcggcat aaatttcatt ccaggtttct tcagacatgg ttgtaccgac
      241 cacctgtacc acccggccag aaatcagcga accaatcatg ccacatatat tcagggcata
      301 accccgggcc agtccatata agaagccggc tgcatacata tcgccggcgc cggtagaatc
      361 cacgcgaata tcgcccgaag gaataacctc atcggttttt tcgccgcttt taatcaaaga
      421 accatcctta cccagtttta caattgcaat atcgcacagg tgagcaatgt cggtcagggc
      481 ttcaaagggg tttttgccgg tgaatgcctt ggcttcttct tcgttggcaa aagtaatatc
      541 gacatagtcg cgaatgattt cgtgcagaaa ttcccgattt tcttccacca cattgaagct
      601 ggccaggtcc agagaaacgg ttaagcccgc ttttttggcc agttgcaagg ctgtacgtat
      661 cagattgtga tcctgaacca gataaccttc tatgtgaaaa atatcgtaac cggtaaacac
      721 cgaactttcc aaatcgccgg ccgacaattc gctcgctgcg cccagatagg tacacaaggt
      781 gcgttcgcca tcgggagata ttaaaacagt acaatgccct gaagcggtat tgctgaattt
      841 caaatgaggt ttaataccgt ttttgcggct gtcttccctg aaaaacgagc caatattgtc
      901 gtcacatact ttgccgataa atccaccttc cagcccaagt ttagctatac ctttaattgt
      961 attggcagcc gaaccgccgg tagccatggt ggtgggaaaa tcattaaaca aagctgtaat
     1021 ttcgtccgac ttttccgcat ccaccagctg catgctccct tttttcaaat taagccctga
     1081 cagaatttca tcgttattta tctgaaacag tatgtcagtc agggcattac ccatagatag
     1141 aacttttttc atttttttta tttcaattat ttctgcaaag atattgcata tttcaagaat
     1201 tcctattatt tttgcactcg caattgagaa attagttgca gaaattcttc cttagctcag
     1261 tcggttagag catctgactg ttaatcagag ggtccttggt tcaagtccaa gagggagagc
     1321 aaaaaacact gaatgtcagt gtgatagaaa aacaaaggac taaaagtggt gtcaaaaccg
     1381 ccttagtcct tttctttttg tgtcatattt aataaaaaaa aagctttcag aagaaagctt
     1441 tttttatttt gaataatgaa aagtcctaac ttctcagttt tgcattcagc ttgttttcaa
     1501 tgttttgctg aatttttcgc atgatgctgt caattacatt gtcggtcatt gttttggttt
     1561 catcctgcag catgaagctt acagcatacg attttttgcc ggtctcaagg tttttgccct
     1621 catacacatc gaacagactt acttttttca acagttttcg ttcggtttca aatgcaattt
     1681 tctcaatttc ggcaaatgtt gtgtttttat ccagcaggaa tgctaaatcg cgtcttacaa
     1741 ccgggaattt tggaagttcg gtgtacttta ctgctgagat attgagttcc gaaaggatgt
     1801 tattccagag caagtcagca taataaactt ctgcgtccac gtcggtcatc tttcgcacct
     1861 gatgatttac aattccgtaa gtggccagta aaacgcccct ggcactgcga atggcaagcg
     1921 cttcggagaa taaatcgtcg gcatattctt cgcttttcaa actgcgaaaa tcaaaaccca
     1981 ttcgtaagaa tatgttctgc acataggctt tcagttcgaa gaagcttgac ttttgagtgc
     2041 caccggacca agaatcgctg aatttgttgc ccgttaccca caatcccagg tgatattctt
     2101 cggaataggc tttcaggtga tcgtcagccg atttattatc agcattgaaa tgataacagt
     2161 tgccaaattc atagaattta agatctgcat tccggtaatt gatgttgcgg gaaatatttt
     2221 caagacctcc gaataataag gtctgacgca tcacattcag gtcagaactc aagggattaa
     2281 ttatttttac gctttgcgtt tcaggataat ctttcagggt ggaataatag ccgccttttg
     2341 tgagtgaatt gttcagcact tcattaaatc cctgagcggt cagctgttcc gagatgagta
     2401 tctgcatttt gtggctatcg ggtttggctg catagctgag tgtcgaaatc agtttatcgc
     2461 caatttccac attattgtat ccatagatgc gcagaatgtc ttcgatcaca tctacatcac
     2521 gttgcacatc cacccggtaa gccggaactt tcaactgaaa tttttctgca tcttccgata
     2581 cgatttccat ttcaagtccg ttcagaattt tcacaatcgt gtcatttccg atttttttgc
     2641 caatcaggtt gtcaattttt tctttattta atgcaacctc aaagggctga actataaccg
     2701 gatactcgtc cactatttcg ctcgaaatag taccgcctgc cacttcctga ataagcatgg
     2761 cggcacgttt gagtgcgtaa atggtgatgt cggggtcgca gccgcgttcg aagcggaacg
     2821 atgcatcggt gtttaatccg tgtcggcgag ccgttttacg gatgtttaca ggatgaaagt
     2881 aagcactttc gaggaatata tttttagtcg tttcggttac tcctgagctc aaaccgccaa
     2941 aaactcctcc aatacacatg ggttcatcgc cgttgcatat catcagatct tcagagctaa
     3001 gtttgcgctc aacgccgtcg agggtggtga aaggtgtacc ttccggcagg ttttttacac
     3061 ataccttacc ggatgtgatt ttgtcggcat cgaaagcatg cagcggttga cccagttcgt
     3121 ggagtatgaa attggtaata tccacaacgt tgttgatagg gcggagacca attgtcatca
     3181 ggctttcctt aagccattgc ggagattcgg ccactgtaac acccgagatg gtgagtgctg
     3241 aatacctcg
//
LOCUS       DAORKP010000202         3203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9062_length_3203_cov_7.327827, whole genome shotgun sequence.
ACCESSION   DAORKP010000202 DAORKP010000000
VERSION     DAORKP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9062_length_3203_cov_7.327827"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..276
                     /locus_tag="PLN75_08885"
     tRNA            190..276
                     /locus_tag="PLN75_08885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Ser,seq:cga)
     gene            428..628
                     /locus_tag="PLN75_08890"
     CDS             428..628
                     /locus_tag="PLN75_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04270.1"
                     /translation="MKNKLCPQCKVRRFQVKNERGEAVVVTVDEQLQIIPIHEGTSLD
                     GFDLEILYCLGCSWKGSVRQLI"
     gene            843..1802
                     /locus_tag="PLN75_08895"
     CDS             843..1802
                     /locus_tag="PLN75_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRG04271.1"
                     /translation="MNIPFEERPAGCTDIMWRYSQNPVIDRYAIPSSNSIFNSAVVPF
                     ENGFAGVFRCDNKAVQMNIFAGFSPDGIHWEINHEPITFKPGNTEMIDSEYKYDPRVV
                     WIEDRYWITWCNGYHGPTIGIGYTFDFKEFFQCENAFLPFNRNGVLFPQKINGKYVLL
                     SRPSDNGHTPFGDIYLSYSPDMKYWGEHRCVMKVTPFPESAWQCTKIGAGPIPIKTDE
                     GWLMIYHGVITTCNGFRYAMGAALLDKNNPEKVLYRTREYLLGPAAPYELTGDVPNVV
                     FPCAALTEGNKIAVYYGAADTCVGMAFGYIDEIIAELKRQDFR"
     gene            2041..>3203
                     /locus_tag="PLN75_08900"
     CDS             2041..>3203
                     /locus_tag="PLN75_08900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRG04272.1"
                     /translation="MKNFLIILSILFASQYALSESLTDYVNPFIGTTNYGTTNPGAIM
                     PHGMVTVAPFNVMGSSTNKFDKDSQWLSTGFEYTNSYVTGLSHNSLSGVGCPELGSLL
                     LMPTTGKLEVDFTKYGSKISQMNAVPGNFSCVFDKYEVVSVSSATVRSGITEFTFPEG
                     ESNILFNLGEGLTNESGSYARFVSDTEIEGMKLQGTFCYNSQAVFPVYFVLQVSKSPA
                     ASGYWKKQRPMTAEAAWDDTAGKYKLYERYNRDIAGDDIGVYFKYNTAKNEKIAVKIG
                     ISYVSIANARENLRKEQPGFDFEKVKKEARESWEKELSRVTVEGGTHDQKVVFYTALY
                     HALIHPNILNDVNGQYPAMESGKILTTDRNRYTVFSLWDTYRNVHQLLTLLYP"
BASE COUNT          913 a          667 c          697 g          926 t
ORIGIN      
        1 ttttcgggtg caaagttacg ataattagtt attacacaaa aataattgac agattatttt
       61 taattatgtc atttgagtta caaataatat caaaaagtgt atataattgc aacaattagg
      121 catattgatt tttatggctt ttatgttgca tcattaaaat aaaaaactta attttgcaaa
      181 tcgattccag gaaagctgcc agagaggtcg atcgggccgc actcgaaatg cggtgaacgg
      241 gtaactgttc cgggggttcg aatccctcgc tttccgcaaa aattaatcaa atcccaaaac
      301 tgatacacgt tgagggattt aattttttcc ggctgtttct gtcagattgg ctgatttata
      361 tgcttttttc acacgtttcc acaacctgtc tgtaatctgt gttctttatt ttttattctg
      421 attaatcatg aaaaacaaac tatgtccgca atgtaaagtc cgccggtttc aggtgaaaaa
      481 cgagcggggt gaagccgtgg tagtcacggt ggatgaacag cttcagatca ttccgataca
      541 cgaaggcacc agcctggatg gattcgatct ggaaattctg tactgtcttg gttgttcgtg
      601 gaaaggcagt gtaaggcaat taatctgatt tttcttcttt tttaccggat ttttgtttca
      661 ggcatgtaat aatgttttcc cggtttttca ttttttttca tttacgggag cttatgaaat
      721 tacctttaaa ttttgtgata aagtaatttt tgttaaaaaa ttattactca atactccaaa
      781 aaccgttttc aattgtacaa tctataaatc tcaaattgta aattttaaat ttatatagta
      841 ctatgaatat ccctttcgaa gagcgtcctg ccggatgtac tgacatcatg tggcgctaca
      901 gccaaaatcc cgtaattgat cgttacgcca ttccttcatc caatagcatt ttcaacagcg
      961 ctgtggtgcc ttttgaaaat ggatttgccg gtgtatttcg ttgcgataat aaagccgtgc
     1021 agatgaatat atttgccggt ttcagccccg atggcatcca ttgggaaata aatcatgagc
     1081 ccatcacatt caagccggga aacaccgaaa tgattgacag cgagtataag tacgacccac
     1141 gcgtggtgtg gattgaagac cgttactgga ttacctggtg caacggctat cacggcccaa
     1201 ccattggcat cggttatacg tttgatttca aagagttttt tcagtgcgaa aatgccttcc
     1261 tgccattcaa ccgaaacggg gttttatttc cgcaaaaaat taatggaaaa tacgtcctgc
     1321 tgagccgtcc gagcgacaat ggacatacgc cattcggcga tatttacctg agttacagcc
     1381 cggatatgaa atactggggc gaacaccgat gtgtcatgaa agtaactcct ttccccgaaa
     1441 gtgcctggca atgtaccaaa atcggtgcag ggcctattcc aatcaaaacc gacgaaggct
     1501 ggctgatgat ctatcacggt gtaattacca cttgcaacgg cttccgctat gctatggggg
     1561 ctgctttact ggacaaaaac aatcctgaaa aagtacttta ccgcacccgc gaatacctgc
     1621 tcggccctgc cgctccttac gaactaaccg gtgatgtgcc taacgtggtt ttcccgtgtg
     1681 ctgcactcac tgaagggaat aaaattgctg tctattacgg tgcagctgat acgtgtgtgg
     1741 gcatggcatt cggatacatc gatgagatta ttgcggaatt gaaaagacag gattttcggt
     1801 agattgtctg agcattttta ggggtgtttg cccttgatat aaattggtat tatctatcta
     1861 acccctccgc tcgttccctg cctgcggcag gcaggctcgc tagtcacctt tatcaaaagt
     1921 ggacatatga gtagcacaat ctaatcttcc cttttgatta agggaagtcc ccgaaggggg
     1981 aagggttaga taaacaaaat ttattttttc aatttaccga aaccaaaaat atttatcaca
     2041 atgaaaaact tcctaattat actttccatt ctttttgctt cgcaatatgc cttatcagaa
     2101 agtctgactg actacgtcaa tccatttatt ggcacaacaa attacggaac taccaatccc
     2161 ggagccatca tgccgcacgg aatggtaaca gtagcacctt tcaatgtgat gggctcttca
     2221 acgaataaat ttgacaagga cagccagtgg ctttctaccg gatttgaata cacgaacagc
     2281 tacgttaccg gcttgtcgca caatagtttg agtggagtag gctgtccgga gttgggaagt
     2341 ctgctgctga tgcccaccac cgggaaattg gaagtcgatt tcacaaaata cggcagtaaa
     2401 atcagccaaa tgaacgctgt gccgggaaac ttttcctgtg tttttgataa atacgaggtg
     2461 gtttctgtga gttcagctac cgttcgttca ggcattaccg agtttacatt tcccgaaggg
     2521 gaaagcaata ttctcttcaa tctgggcgaa gggctgacca acgaaagtgg ttcgtacgcg
     2581 cgatttgtaa gcgatacaga aattgaagga atgaagttac aaggcacatt ttgctacaat
     2641 tctcaggctg tttttccggt ttatttcgtg ctgcaagtga gcaaatcacc tgcggcatcc
     2701 ggttactgga aaaaacaacg cccgatgact gccgaagctg catgggacga tacagcggga
     2761 aaatacaaac tttacgaacg ttacaatcgc gatattgccg gcgatgatat tggcgtttat
     2821 tttaaatata ataccgcaaa aaacgaaaaa attgctgtta aaatcgggat ttcttatgtg
     2881 agcatcgcca atgcgcgtga aaatttgaga aaagaacagc ccggttttga ttttgagaaa
     2941 gtgaaaaaag aagcccgcga aagctgggaa aaagagttgt cgcgcgttac cgttgaaggt
     3001 ggtactcacg atcagaaagt ggtattctac accgcgcttt atcatgcact tattcacccc
     3061 aacatcctga acgatgtgaa cgggcaatat ccggctatgg aaagcggaaa gatactgacc
     3121 accgacagaa accgttatac cgttttctcg ctctgggata cgtaccggaa tgtgcatcaa
     3181 ctgcttacgc tgctttatcc cga
//
LOCUS       DAORKP010000203         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9451_length_3125_cov_8.822150, whole genome shotgun sequence.
ACCESSION   DAORKP010000203 DAORKP010000000
VERSION     DAORKP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_3125_cov_8.822150"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            521..1297
                     /gene="lptB"
                     /locus_tag="PLN75_08905"
     CDS             521..1297
                     /gene="lptB"
                     /locus_tag="PLN75_08905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRG04273.1"
                     /translation="MVLRTEHLYKKYGKRTVVNNVSIHVKQGEIVGLLGPNGAGKTTT
                     FYMATGLVTPNSGKIYLNEEDITKFPVFRRAQKGIGYLAQEASVFRKMTVEDNISSIL
                     QMTKFSKAYQKEKLETLITEFNLGHVRKNIGDRLSGGERRRTEIARCLAIEPKFIMLD
                     EPFAGVDPIAVQDIQDIVWRLKDKNIGILITDHNVDETLSITDRAYMLFEGKIMFQGT
                     SEELAANPIVREKYLGRDFQLKVKTRIDTPKIQNDISETF"
     gene            1325..2293
                     /locus_tag="PLN75_08910"
     CDS             1325..2293
                     /locus_tag="PLN75_08910"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRG04274.1"
                     /translation="MVLYPQNKTSDFQDESSGGQRGLLHIIGSGSALPTKNNFPSSQM
                     VEMRNKQFMIDCGEGTQIRLRQMGLKTNRLGHIFISHLHGDHVFGLMGLITTFGMLHR
                     TADLHIHAHPDLEALLTPHLRYFADGSPFQVIFHPFNPAESETIYDDRSVRVTTIPLK
                     HRVPACGFLFEEKTGERHIKRDMIDFYGVPVSRILKIKQGDDFVTDEGEIIPNDIFTT
                     PPTPPIRYAYCSDTAYSEKIISVIEGVDCLYHEATFAEDEALRARQTMHSTARQAATI
                     ASKANVKKLIIGHFSARYANKNVLLNQAKDIFENTFLAEDMKTFEF"
     gene            2369..2563
                     /locus_tag="PLN75_08915"
     CDS             2369..2563
                     /locus_tag="PLN75_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04275.1"
                     /translation="MNRVTKILLILNGLLASLAASASDVSATAMEETSLFYTQLWFWV
                     AVGIVFFFLLVILLRGSNRS"
     gene            2743..>3125
                     /locus_tag="PLN75_08920"
     CDS             2743..>3125
                     /locus_tag="PLN75_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRG04276.1"
                     /translation="MIYIVNQSNKPDYNIALEEYCFKKLLHHDKIFILWINEPAIIVG
                     KHQNTIEEINPEYVKEHGIHVVRRITGGGAVYHDLNNLNYTIISNENKGVDFDFKTFS
                     QPVIDTLAELGVTAEFTGRNDLVING"
BASE COUNT          967 a          713 c          634 g          811 t
ORIGIN      
        1 aattttttgc ttactttttg tttcaagaca aaaagtaagt cagggtctaa ggggcagaag
       61 cccctgttcc tgaaaaaatg tatatctatc tgactatcag atactatata ttcttaatct
      121 tttgatttat aaactaatat gaacatgcga aagttcaatt acataaataa accgaatgtc
      181 aggcaggagc tttcaagcgg ttgatgagcc tataaggtgg gtaacgtgag tgtgactgat
      241 tttattgtac ctacaaacac ataacaagct aaaatttcaa cttcacaact ctcattgctg
      301 cttttattct taaaaaatca aataaagagc tggctgtctg tgtcaattca ttcagaattt
      361 tgtacttttg ttgctccaaa ataaagaatg agctatggaa gaagataaat cagcaatcgt
      421 cctgacagaa aataccactt ttgatacatt tgaagaagaa acagctactc atcagatact
      481 ggaagatacc gatataagca gtgtgcccga agttacttct atggtgcttc gtaccgaaca
      541 tctgtataaa aaatatggaa aacgtacggt tgtgaacaac gtatccatcc acgtaaagca
      601 aggtgaaata gtaggtttgc tcggaccaaa cggtgccgga aaaaccacta ccttctacat
      661 ggctaccggg cttgtgacac cgaactcggg caaaatatat ctgaacgaag aagacatcac
      721 caaatttcct gtttttcggc gcgcccagaa aggcatcgga tacctggctc aagaggcatc
      781 tgttttcaga aaaatgacgg tggaagacaa catcagttcc atactccaga tgacgaaatt
      841 cagcaaagct tatcagaaag aaaaactcga aacgctcatt accgaattca acctcggaca
      901 tgtgcgtaaa aacattggcg accgcctttc gggaggtgag cgccgccgca ccgaaatagc
      961 ccgctgcctg gccatagagc caaaattcat catgctcgac gaaccttttg ccggcgttga
     1021 ccccattgcc gtacaggata ttcaggacat cgtgtggcgg ctgaaagata aaaacatcgg
     1081 aatcctgata accgaccaca atgtggacga aacgctcagc attaccgacc gggcttacat
     1141 gcttttcgaa ggaaaaatca tgtttcaggg tacttcggaa gaacttgccg ccaatccgat
     1201 agtacgcgaa aaatacctgg ggcgcgactt ccagctgaaa gtaaaaacaa ggattgatac
     1261 tccaaaaatc caaaacgaca tttctgaaac tttctaacgc agtcgtgttg taacataata
     1321 ttcaatggtt ttatacccgc aaaataaaac ttctgatttc caagatgaaa gttccggagg
     1381 tcagcgcgga ctgctccaca tcatcggttc gggttctgcg ttgcctacca aaaacaattt
     1441 tccatcgtcg cagatggtcg aaatgcgcaa taagcagttt atgattgact gcggcgaagg
     1501 cactcagatc cgactccggc aaatgggact caaaactaac cggctgggtc atattttcat
     1561 ttcacacctg catggcgatc atgtattcgg attgatgggg ctcatcacca cattcgggat
     1621 gctccaccgt actgccgatc tgcacatcca cgctcacccc gatctggaag cacttctcac
     1681 acctcacctc aggtattttg ccgatggttc gccgtttcag gtcattttcc atccgttcaa
     1741 tccggctgaa agcgaaacaa tttacgacga ccgttctgtc agggtaacca ccatcccgct
     1801 gaagcaccgc gtacctgctt gcggatttct gtttgaggaa aaaaccggcg aacggcacat
     1861 caaaagggat atgattgatt tttacggcgt tccggtgagc cggattctga aaataaaaca
     1921 gggcgatgat tttgtaaccg atgaaggtga aatcattcca aacgatattt tcaccacacc
     1981 tcccactcca cctatccggt acgcatattg ctcagataca gcctattccg agaaaataat
     2041 ttctgttatt gaaggtgttg actgcctgta tcacgaagct acatttgccg aagacgaagc
     2101 cttgcgggca cggcaaacca tgcattccac agcccgccag gctgccacca tagccagcaa
     2161 agcaaatgtg aaaaaactca tcatcggtca tttttcggcc agatatgcca acaaaaacgt
     2221 tttgctgaac caggcgaaag acattttcga aaatacgttt ctggctgagg atatgaaaac
     2281 cttcgaattt taaactttta gcgcttcccc gaaaaaactt ttcagcattt tacttgttta
     2341 tggattatca ttcatcgaca aatttaccat gaatcgtgtt acaaaaattc ttctgatatt
     2401 aaacggactt ctcgcatcgc tggcagcctc tgcttccgac gtatcagcaa ctgcaatgga
     2461 agaaaccagt ctgttctata ctcaactttg gttttgggtg gctgtgggca ttgtgttttt
     2521 ctttttgctg gtgattttac tgcgtggaag caatcggagt taaccgtttt tttgttcaga
     2581 aaaaaacata catgactttt ttacagaaag aaagaatcaa tttgactgaa aaaaacaatc
     2641 aggctcaaat caatttctgt cttcgaagat agtctgaaag agattttgaa ataataactg
     2701 ttcattaaag aaacaataac tacatcatca aacattcgga aaatgattta catcgtcaac
     2761 caatccaaca aacccgatta taacatcgct cttgaagaat attgctttaa aaagctattg
     2821 catcacgaca agattttcat tctctggatc aacgagccgg ctatcatcgt gggaaaacac
     2881 cagaacacca tcgaagaaat caatccggaa tatgtgaaag aacacggcat acacgttgta
     2941 aggcgtataa cgggcggcgg agccgtgtat cacgacctga acaacctgaa ctataccatc
     3001 atttcgaacg aaaacaaagg agtggatttt gatttcaaaa ccttttcgca accggtcatc
     3061 gatacgcttg ccgagctggg cgtgactgcc gaattcaccg gccgcaacga cctggttatc
     3121 aatgg
//
LOCUS       DAORKP010000204         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9616_length_3095_cov_8.276645, whole genome shotgun sequence.
ACCESSION   DAORKP010000204 DAORKP010000000
VERSION     DAORKP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_3095_cov_8.276645"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1077)
                     /locus_tag="PLN75_08925"
     CDS             complement(<1..>1077)
                     /locus_tag="PLN75_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRG04277.1"
                     /translation="PTGDQPEAIRQLVDGVNMGIPYQTLLGVTGSGKTFTIANVVEQV
                     QKPTLVLSHNKTLAAQLYSEFKSFFPQNAVEYFVSYYDYYQPEAYLPVTDVYIEKDLS
                     INQELEKLRLATTSALLSGRRDVLVVSSVSCLYGIGNPDDFTENVIEIRRGQKLVRNV
                     FLRQLVDSLYTRNEIELNRGNFRVKGDTVDIFLAYADFILRIVFWGDEIEEIQTVESI
                     DYHVIERFESYKIYPASIFVTTKPRIMNAIQLIESDLEVQVGNFRAQGKDYEAKRLYE
                     RVTYDLEMIKELGYCSGIENYSRYFDGRSPGSRPFCLLDYFPDDFLLVIDESHVTLPQ
                     VHAMFGGDAARKTNLIEYGFRLQAA"
     assembly_gap    1078..1177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1178..2053)
                     /locus_tag="PLN75_08930"
     CDS             complement(<1178..2053)
                     /locus_tag="PLN75_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRG04278.1"
                     /translation="MKNPVLATQNSPFWGLGGLTNYHSHSVFCDGRAPMEDFVRFAIA
                     KGVKKYGFSSHAPLPFHTSWNMNLDDFTYYRREFYRLKEKYEGQIELFLGLEVDYIEG
                     VFDAKNDLYDMSDIEYKIGSVHYLDPLPEGGFFSVDGKLTNFQWRLNEIYDGDIRMAT
                     KRYFEISSNMVRKGGFDIVGHLDKISQNGMNCPGFDKTATWYVDLVTDYLELIKEQDM
                     MIEINTKSYLELGITYPDVQFFPVIKELEIPVMVNSDCHYPDKITDGFDAVYELLKQA
                     GFQSEMELAGKEWVES"
     gene            complement(2158..2379)
                     /locus_tag="PLN75_08935"
     CDS             complement(2158..2379)
                     /locus_tag="PLN75_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRG04279.1"
                     /translation="MDLRKKVFRNAGNTSIAGVCGAIADFLEVNPKIVRIAYIVFTVL
                     TAIFPGVFLYLFLMLLIPREEIATEQVDE"
     gene            complement(2381..2578)
                     /locus_tag="PLN75_08940"
     CDS             complement(2381..2578)
                     /locus_tag="PLN75_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRG04280.1"
                     /translation="METKKKLTRSKDRILAGVCGGMAEYFGIDATIVRIGYVLISILS
                     AAFPGIIVYLILMLIMPNSEQ"
     gene            complement(2738..>3095)
                     /locus_tag="PLN75_08945"
     CDS             complement(2738..>3095)
                     /locus_tag="PLN75_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRG04281.1"
                     /translation="ELKTKAGIKGFLSKSVDKNELMTAIMTVHNGEEYFSEEAEREIG
                     NYEKISKELEQITLTKREIEIIRLIDNGLINKEIAKKLFISEQTVTTHRKSILRKTGA
                     HNVSTLLNFARKAQII"
BASE COUNT          825 a          703 c          589 g          878 t
ORIGIN      
        1 gctgcctgca agcggaaacc gtattctatc agattggttt tacgggcagc atcgccacca
       61 aacatggcat gtacctgcgg taacgtcaca tgactctcgt caattaccag cagaaaatca
      121 tccggaaaat aatcgagcag acagaacgga cggcttccgg gcgaacgtcc gtcgaaatag
      181 cgggaataat tctcaatgcc cgagcaataa cccagttcct taatcatttc caaatcatac
      241 gtaacccgct cgtaaagccg ttttgcttcg taatctttac cttgtgccct gaaattacca
      301 acctgcactt ccaaatcgct ctcaatcaac tgaatagcat tcataatacg cggtttggtg
      361 gttacgaaaa tgcttgccgg ataaatcttg taactttcaa aacgttcaat tacatgataa
      421 tcaatgcttt ctacggtttg aatttcttca atttcgtcac cccaaaaaac aattcgcagt
      481 ataaagtcgg cataagccag aaaaatatct accgtatcgc cttttacccg gaaatttcca
      541 cggttcagtt ctatttcatt gcgggtgtag aggctgtcaa cgagctgacg caggaaaaca
      601 tttcgcacca gtttctgacc gcggcgaatt tcaatcacat tttcggtgaa gtcatcggga
      661 ttaccgatgc catacaggca cgaaaccgac gaaaccacca gcacatcacg cctgcccgac
      721 aacagcgccg aggtggtagc cagacggagt ttttccagtt cctgattgat ggaaagatct
      781 ttttcgatgt acacatcggt caccggcaga taggcttcgg gctgataata atcgtaatac
      841 gacacgaaat attcaaccgc attctgaggg aaaaaacttt tgaactcgct gtaaagctgg
      901 gctgccaggg ttttgttgtg gctcaataca agcgttggtt tttgcacctg ctccaccaca
      961 ttggctatgg taaatgtttt acccgaaccg gtcacaccaa ggagagtctg gtacggaatg
     1021 cccatgttga ccccgtccac cagctgcctg atggcttccg gctggtcgcc ggtaggtnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct ttccacccac tcttttcccg
     1201 ccagttccat ttcagattga aaacctgcct gcttcaacaa ttcataaacc gcgtcaaatc
     1261 catctgtaat tttatccgga taatggcaat cggaattgac catcacaggg atttcaagtt
     1321 cttttatcac cggaaaaaac tgcacatcgg gatatgtgat gccaagttcg aggtaagact
     1381 tggtgttgat ttcaatcatc atatcctgct cttttatcaa ctccaggtaa tccgtaacca
     1441 aatccacata ccacgttgcc gttttgtcaa aaccgggaca attcatcccg ttctgcgaaa
     1501 ttttatccaa atgccccaca atgtcaaatc cgcctttgcg aaccatgttg gaacttattt
     1561 cgaagtatcg ttttgtagcc atgcggatgt caccgtcgta aatttcgttc agtctccact
     1621 gaaaattagt aagttttccg tccacactga agaaacctcc ctcgggcaac ggatcgagat
     1681 aatgcaccga accgattttg tattcgatat cgctcatatc gtacaggtcg ttttttgcat
     1741 caaaaacacc ttcaatatag tcaacttcca gcccgagaaa aagttcaatc tgcccttcat
     1801 atttctcttt caatcggtaa aactccctgc ggtagtaagt aaaatcatct aagttcatat
     1861 tccacgaagt gtgaaacgga agcggtgcgt gcgacgaaaa accgtatttc ttcacgcctt
     1921 tggctatggc aaagcggacg aaatcctcca tcggagcccg tccgtcgcag aaaacactgt
     1981 ggctatggta gttggttaag ccccctaacc cccaaaaggg ggaattttga gtggctaaaa
     2041 cgggattttt caaaatatat agaaatttca gggattaatc aatcagactc acgggataac
     2101 gttcattcac acattttcac tttgagggag aaaatcacgt aggaaatggg ggttctttta
     2161 ctcgtcaact tgttcagttg caatctcttc cctcggaatt aaaagcatca aaaacaaata
     2221 tagaaaaaca cccgggaata tggctgtgag cacggtgaac acaatgtatg ctatccgtac
     2281 tattttcgga ttaacttcca ggaaatcggc tattgcgccg caaactcccg caatgctggt
     2341 gttgcctgca tttctgaata ctttttttct tagatccata tcactgttcg gaattgggca
     2401 tgatcaacat cagtatgaga tacacgataa taccgggaaa tgcggctgat aatatcgaaa
     2461 tcagtacgta tccaattcgt actatggttg catcaattcc aaaatactca gccataccgc
     2521 cacaaactcc cgccagtatg cggtctttgg agcgggttaa ttttttcttc gtttccatac
     2581 ctgttctttt tttagcagaa aatacctttt acaaagataa aacaaatttt catcaggcgg
     2641 tgtggatttt tcaggaaaag ataaagggat tttctaactg ttcaatccgg aatctgactt
     2701 acatactcat tatacccgga aatgatgggt tccgaattta aatgatttgt gcttttcttg
     2761 caaaattcag aagtgtactc acattgtgag ctccggtttt acgcagaata gatttccgat
     2821 gtgtggttac agtttgctcg ctgataaaaa gtttttttgc tatttctttg tttatcagcc
     2881 cattatctat caggcgaata atttcaattt cacgtttggt aagggttatt tgctccagtt
     2941 ctttgctgat tttttcgtaa tttcctattt cacgttcagc ttcttcgctg aaatattctt
     3001 caccgttatg tacagtcatt atggctgtca tcagttcgtt tttatctaca gatttcgaca
     3061 gaaaaccctt gattccagct tttgttttga gttcg
//
LOCUS       DAORKP010000205         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9624_length_3093_cov_9.729427, whole genome shotgun sequence.
ACCESSION   DAORKP010000205 DAORKP010000000
VERSION     DAORKP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_3093_cov_9.729427"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLN75_08950"
     CDS             complement(<1..407)
                     /locus_tag="PLN75_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04282.1"
                     /translation="MKKNYLIAIAVFLSTSVFAQLSLVTTFTAANELKQEQIREDNYF
                     QGHVFYFGQGSTTSSQLYKTDGNTITLVKDFGPVYPEGETATGISISNFNQTSDKLFF
                     TRYSMRFGTTAPLDAISELWTSDGTTEGTIKLAS"
     gene            complement(655..1848)
                     /locus_tag="PLN75_08955"
     CDS             complement(655..1848)
                     /locus_tag="PLN75_08955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRG04283.1"
                     /translation="MPGVSERLASLSPSATFAMAQKSNELKAQGVDVISLSVGEPDFN
                     TPDHIKEAAKKAIDDNFSFYTPVVGYLELRKAIVEKLKNENGLAYTPAQIVCSNGAKQ
                     SLCNVLMAVVGKGDEVLIPAPFWVSYPEMVKIADATPVIIYSDIESDFKITPEQLEAA
                     ITPKTKVLMLCSPSNPTGSVYTREELKELADVVAKYPEIIVLSDEIYEHINYLDKHVS
                     IAEFPEIFDRTVIVNGVSKGYAMTGWRLGWIAGPKWIADACNTLQGQYTSNPSSIAQK
                     AAEAAYTGDQQCVADMRVAFERRKNLVVKMAREIPGLKVNEPKGAFYVFPDCSYYFGK
                     SYNGTTVNNPEDLALFLLAEAHVACVGGTDFGAPNCIRMSYATSDEKLVEAFTRIKEA
                     LGKLK"
     gene            complement(1964..2797)
                     /locus_tag="PLN75_08960"
     CDS             complement(1964..2797)
                     /locus_tag="PLN75_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRG04284.1"
                     /translation="MNKFSNPLNAFIESAFSPKPYKIGIALSGGGIKGMCHAGVLKAL
                     EEAGVKPDIISGVSAGAIVGALYADGHSPDEIAEIFEHVEFRKMTKLQVPDGGFFRTE
                     RFEKYLGSKLRAKTFEDLKIPLKIVATNLDRGESVTFSSGKLLPVIMASTTVPILFPP
                     RKINSENYVDGGVFKNFPVSIIRDECEYLIGINASPLVVTEYKKSILNIAMRTYNFMF
                     KANIIADKAMCDFLIEPVDMGNYDTFETEKSREIYELGYHTARELIQEQLKQLKMTKT
                     Q"
BASE COUNT          797 a          691 c          649 g          956 t
ORIGIN      
        1 gaggccaatt tgattgtacc ttcggttgta ccatctgaag tccataattc tgaaatggca
       61 tccagaggag ctgtagtacc gaaccgcatg gaatatcggg taaaaaaaag tttatcggaa
      121 gtttggttaa aattcgaaat ggaaattccc gtagcggttt cgccttcggg atatacagga
      181 ccaaaatctt tcaccagagt aattgtgtta ccgtcggttt tatagagctg actgcttgta
      241 gtgctcccct gtccgaaata aaacacatga ccctggaaat aattatcttc acggatttgc
      301 tcctgtttca gttcgttggc agcagtaaaa gttgtgacta aagaaagctg agcaaacaca
      361 cttgtgctca gaaaaaccgc aattgcaatc aggtaatttt ttttcatttg taattcactt
      421 taagttacta attttgtttc aattgaatga ttgcaaattt attgtaccgt taaaatgaat
      481 gcaataccca ctaatgggta tttttaatga aatttaccct tatttaaaaa caaaaaactc
      541 tgcccaattt taaaaatccg gcagagttta atttgtaatg ctgttgctac gtagcaatat
      601 ttgttccccc tgaggacttt tcccgcccta tcaggttcgg gaggccaggg gttgttattt
      661 caatttcccc agcgcctcct taattctcgt aaacgcttcc accaatttct catcggaagt
      721 ggcatacgac atgcggatgc agtttggtgc gccgaaatcg gttccgccta cgcaggctac
      781 gtgcgcttcg gcaagcagga aaagtgccag gtcttcggga ttattgacgg tggttccgtt
      841 gtatgatttt ccgaaataat aactgcaatc cgggaacaca tagaatgctc ctttgggctc
      901 gttcactttc aatccgggaa tttcgcgggc cattttcacg accaggtttt tgcggcgttc
      961 gaatgccacg cgcatatcag ccacgcactg ctggtcaccg gtgtaggcgg cttcggcagc
     1021 tttctgggca atggacgatg ggttggaggt gtactgtccc tgcagcgtgt tgcaggcatc
     1081 ggcaatccat ttcggtccgg caatccatcc aagacgccag ccggtcatgg cgtaaccttt
     1141 cgaaactccg ttgacaatta ccgttcggtc gaaaatttcc ggaaattcgg caatgctcac
     1201 gtgcttgtcg aggtagttga tgtgctcgta gatttcatcg gaaaggacaa tgatttcggg
     1261 atattttgcc actacatcgg ccagttcttt cagctcctca cgggtgtaaa cgctcccggt
     1321 cgggttggac ggcgagcaga gcatcagcac ttttgttttc ggggtgatag ccgcttcaag
     1381 ctgctccggc gtgattttga aatcgctttc gatgtcggaa tagataatca cgggagttgc
     1441 gtcggcaatt ttcaccatct ccggatagct tacccagaac ggcgccggta tcagcacttc
     1501 gtcgcctttt cccaccacag ccatcaggac attgcaaagc gattgtttgg ctccgttgga
     1561 gcaaacaatc tgtgcaggtg tgtaagccag accgttttcg tttttcagtt tttctacaat
     1621 ggcttttcgc agctcaaggt aacccaccac cggcgtataa aacgaaaaat tatcgtcaat
     1681 agccttttta gcagcttctt tgatgtggtc gggcgtgttg aaatcgggtt cacccacgct
     1741 tagactgatt acatctactc cctgagcttt cagctcgttg cttttttgtg ccattgcaaa
     1801 tgtggcagat ggcgacagcg acgccaatcg ttcggatact ccaggcataa taaattgtat
     1861 ttttgaatta taaagtattt ttgtcaatat ttttgtgcaa atttaatcat aataatttaa
     1921 tcagcatcgt ttttcaatca aaaaactaca tattttcgta atttcactgt gtttttgtca
     1981 ttttcaactg tttcaattgt tcctgtatca attctcttgc cgtatgatat cccagttcgt
     2041 atatttcgcg gcttttttca gtttcaaaag tatcgtaatt tcccatatcc accggctcaa
     2101 tcaggaagtc gcacatggct ttatcggcta tgatattggc cttgaacata aagttataag
     2161 tgcgcatggc gatattgaga atggattttt tgtactcggt aactaccagc ggactggcat
     2221 tgatcccgat taaatattcg cattcatccc gaattatgct caccggaaag ttcttaaaaa
     2281 caccgccatc cacgtagttt tcactgttga ttttcctggg tggaaaaagg atgggaacgg
     2341 tagtggaagc cataatcaca ggaagcaatt ttccacttga aaatgtaaca ctttcgcctc
     2401 tgtctaaatt agtggccacg attttcagcg gtattttcaa atcctcaaat gttttagccc
     2461 gcaatttact cccaaggtac ttttcaaatc gttctgtacg aaaaaaacca ccgtcgggca
     2521 cttgcagttt cgtcattttc cggaattcca catgttcaaa aatctcagca atctcatccg
     2581 gcgaatgtcc gtcggcatat aatgcaccta caattgcacc agcactcact cccgaaataa
     2641 tgtcgggctt aacacctgcc tcttcgagcg ctttcaacac gcccgcatga cacattccct
     2701 taattccgcc gccactcaaa gctataccga ttttgtatgg tttgggtgaa aatgcagatt
     2761 cgataaatgc gtttaaaggg ttggaaaact tgttcatagt ctaaaatgaa attctatttc
     2821 ttccacaaaa ttacggtttt ttcttttaat aaaacagttt atttgtatct ttgtgtaaca
     2881 gatggcagat ttcgaaagtc atatattggt ttattttgaa aatcagaaaa cgtcgtttga
     2941 caaagttaaa gaaacttata tggatacaat tctcattata ctggcatttg cctgcatcat
     3001 cgttgggatt atcggatcgg ttttgcctgt tctacccggc gttccgctta gttacggcgg
     3061 cattttactc atgcatttca ccgataaagt aca
//
LOCUS       DAORKP010000206         3091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9634_length_3091_cov_11.109354, whole genome shotgun sequence.
ACCESSION   DAORKP010000206 DAORKP010000000
VERSION     DAORKP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9634_length_3091_cov_11.109354"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLN75_08965"
     CDS             <1..300
                     /locus_tag="PLN75_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRG04285.1"
                     /translation="HGDLWGGNYLIAADGTPYLIDPAVCYGHSMMDVAMSRLFGGFDA
                     EFYDSYHQIIPQPQFYREQIDIYQLYYLLVHLNLFGRSYRSSVVDILERYFAPVR"
     gene            complement(323..589)
                     /locus_tag="PLN75_08970"
     CDS             complement(323..589)
                     /locus_tag="PLN75_08970"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HRG04286.1"
                     /translation="MFKFLFGLILFFVLLVLLAGALGIGFLRSIFGIGKQQSSRRSDD
                     TTHTPSNPDKDKIFGDHEGEYVDFEEIDTPESPEGNLKESEKEE"
     gene            complement(605..1552)
                     /gene="metF"
                     /locus_tag="PLN75_08975"
     CDS             complement(605..1552)
                     /gene="metF"
                     /locus_tag="PLN75_08975"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRG04287.1"
                     /translation="MSLVEKIKSSQNTAFSFELLPPLKGNGISGVYRTIDTLKEFDPK
                     YINITTHRSEMIFRENDKGLYEQVSVRKRPGTVAVAASIKNRYNIPVVPHIICSGFTK
                     EETEYVLIDLKFLDINDLLLLRGDKAKHETGFVPTGHSFATELQEQVNRFNDGYFLDG
                     SQMKVIDEPFSYGMACYPEKHEESPNMETELYWAKKKVDNGAEYLVTQMFFDNRKYYD
                     FVEKCREIGIDVPIIPGLKPITFMNQLTVLPRIFKSEIPEEFARELRKCKTDEAAAEV
                     GVEWCTEQARDLISHKVPSIHFYSLMATESVKRVAQQVY"
     gene            1807..2277
                     /locus_tag="PLN75_08980"
     CDS             1807..2277
                     /locus_tag="PLN75_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HRG04288.1"
                     /translation="MKNKRERLNVISEILRFQVMGSQEELLDLLKERGYDVTQATLSR
                     DLKLLKVAKTPLSNGAYKYVLPPQIKTIPDELSSHNFTSHGAVLRLDFSGQLAIVKTK
                     PGYASAIAWDIDNKATNEVLGTIAGDDTVLLIPREGVTKEEIMKVINLIFQENN"
     gene            2299..3009
                     /locus_tag="PLN75_08985"
     CDS             2299..3009
                     /locus_tag="PLN75_08985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRG04289.1"
                     /translation="MKKDNNEVDEISIFVADESHLPYVQTILTTIEEAAKIRGTGIAK
                     RTPDYIELKMRERKAIIALTGDGQFAGFCYIETWGDKKFVANSGLIVVDTFRGHGLAK
                     RIKQKAFQLSRERYPDAKIFGLTTGLAVMKINSELGYRPVTFSELTDDEAFWKGCQSC
                     VNYDILQRTHRKHCLCTGMLFDPAWNKESEEEKSKNTANTLVVKPVKKPVKIVAKVVS
                     PKKKSKKKPGSKLMINLL"
BASE COUNT          909 a          633 c          650 g          899 t
ORIGIN      
        1 cacggcgatt tgtggggtgg aaattatctg attgctgccg atggaacacc ttatctgatt
       61 gatccggctg tctgttacgg acattcaatg atggatgtgg ccatgtcgcg gttgtttggt
      121 ggatttgatg cagagtttta tgattcatac catcaaatta tcccacaacc ccaattttat
      181 cgggaacaaa ttgatattta tcagctttac tacctgctgg tgcatctgaa tttattcggg
      241 cgaagttacc gaagcagtgt ggtggatatt ttagaaaggt actttgcccc agtccgctaa
      301 aaggtttttt actaaatacc tattattctt ctttttcgct ctctttcaag tttccttcag
      361 gggattcagg agtgtcaatt tcctcaaaat caacgtattc tccctcatga tcgccgaaaa
      421 tcttatcttt atccggattt gaaggggtgt gggtagtgtc atccgaacga cgcgacgatt
      481 gttgtttgcc aataccgaat atcgaacgga gaaaaccaat accaagcgct cctgcaagca
      541 acaccaggag tacaaaaaag agaatcagtc cgaataaaaa tttaaacata acaggttagt
      601 ttgattaata tacttgttgg gctacccgtt ttacgctttc tgtagccatc aggctgtaga
      661 agtgaatact gggaacttta tgggaaatca gatccctggc ttgctccgtg caccattcca
      721 cacctacttc tgcagctgct tcatcggttt tgcacttgcg gagttcgcgg gcaaattctt
      781 ccggaatttc cgatttgaat atcctgggca gcaccgtcag ctggttcatg aatgtgatgg
      841 gcttgagtcc cggaataatc ggtacatcaa taccaatttc gcggcatttc tccacaaaat
      901 cgtagtattt ccggttgtca aaaaacatct gtgtgaccag atattctgct ccgttatcca
      961 ctttcttttt tgcccagtaa agttccgttt ccatattggg cgattcttcg tgtttctccg
     1021 ggtagcaggc cattccataa gaaaacggtt cgtcgatcac tttcatttgc gaaccatcca
     1081 aaaaataccc atcattaaaa cgatttacct gctcttgtag ttcggttgca aaagaatgcc
     1141 ccgttggaac gaatccggtt tcatgctttg ccttatcgcc gcgcagcagc agtaaatcat
     1201 tgatatccag aaatttgagg tctatcagta catattcggt ttcttctttg gtaaatccgc
     1261 tgcaaataat gtgcggaact accggaatgt tgtaccggtt cttgatggag gctgccacag
     1321 ctaccgttcc gggtctttta cgcaccgaaa cctgttcgta cagcccttta tcgttctctc
     1381 tgaatatcat ttcagacctg tgtgtggtga tattgatgta tttcggatca aattctttca
     1441 acgtatcaat tgttctatac acaccgctga taccgtttcc tttcagcgga ggcagtaatt
     1501 cgaacgaaaa ggccgtgttc tgtgaggatt ttattttttc gactaagctc atgatttgca
     1561 ttttcagact acaaaattac aaagaatttc cgaataagga attttcggaa cagactacaa
     1621 tcacatttaa tacctgtttt tcatattctt gaaaataaat gcagaaaaag ttgttggata
     1681 tgcataaaat ccgtatattt gcaccgtttt ttacaataaa tatactaaaa atgaaattaa
     1741 tcccgattta tttcggagcg aattctcccg atgtatcgga atcaacaaaa aaacaataaa
     1801 ttacgtatga aaaataaaag agaacgttta aatgtcattt cagaaatcct tcgctttcag
     1861 gtgatgggaa gtcaggaaga attactggat cttctgaaag aaagagggta tgatgtaact
     1921 caggctacct tatcgcgcga tcttaaattg ctgaaggttg caaaaactcc actttcaaac
     1981 ggagcttaca aatacgtttt gccaccgcag atcaaaacaa ttccggatga attgagttcg
     2041 cacaatttca cttcacacgg tgccgtattg cgtctggact tctccggtca gttggctata
     2101 gtcaaaacca aaccgggtta tgcaagtgct attgcctggg atattgacaa taaggcaact
     2161 aacgaagtgc ttgggaccat tgcaggtgat gataccgttt tacttattcc gcgcgaggga
     2221 gttacaaaag aagaaatcat gaaagtaatt aatctgattt ttcaggagaa caattagcag
     2281 aggtttttta cataagaaat gaaaaaagat aacaacgaag ttgatgaaat ctccatcttt
     2341 gtggcggatg aaagtcatct gccatacgtt cagactattc ttaccactat agaggaagca
     2401 gccaaaattc gtggcaccgg aattgccaaa cgaactcccg attacatcga gctgaagatg
     2461 agggaacgaa aagctattat tgctctgacc ggcgatggac aatttgccgg attctgttat
     2521 attgagacct ggggtgataa aaaatttgta gccaattcgg gacttatcgt tgtagatact
     2581 ttccggggac acggcctggc aaagagaatc aagcaaaaag catttcagct ttcgcgcgag
     2641 cggtatcccg atgccaaaat cttcggactt accaccggcc ttgctgtgat gaaaatcaat
     2701 tctgaattgg gctatcgtcc cgttacattc tcagaactca ccgacgacga agcattctgg
     2761 aagggatgcc agagctgtgt gaattatgac attctgcaac gtactcaccg caagcactgc
     2821 ctgtgtaccg gaatgctttt tgaccctgcg tggaataaag agagcgagga agaaaaatca
     2881 aaaaatactg ccaacacact ggtagtgaaa cctgtaaaaa aaccggttaa aatagttgca
     2941 aaggttgttt ctcccaaaaa gaaaagcaaa aagaaaccgg gtagtaagct gatgattaac
     3001 ttattgtagt atttgaatta aaacaaaatt gaaaaaatga aagaaaaagt agttttagca
     3061 ttcagcggtg gattggacac ttcattctgc g
//
LOCUS       DAORKP010000207         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9780_length_3065_cov_9.880731, whole genome shotgun sequence.
ACCESSION   DAORKP010000207 DAORKP010000000
VERSION     DAORKP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9780_length_3065_cov_9.880731"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1295)
                     /locus_tag="PLN75_08990"
     CDS             complement(63..1295)
                     /locus_tag="PLN75_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRG04290.1"
                     /translation="MTNHKFALWVARRYLFSKKSHNAINVISGISAAGVSIGTAALIC
                     VLSVFNGFGTLIQGMFSAFDPDLKISLVEGKTFNVNTPDLLKVKKLGSVAHYAEVIEE
                     TALLSFSDRQMPARIMGISENFNKVSSIDSIMYDGSFVLYDGAFQRAVVGIGVANKLG
                     INARVINPLQILAPKRTGRINMVRPESSFNPASAYISGVFVVNQPEYDDQYVLTSLSL
                     ARELFEYDENTVSGLEIKVKPTAEIQKTKTEIRKILGERFKVLDRYEQQEDFYKITKM
                     EKWITYLILCFILLIATFNIIGSLSMLILEKKEDIRVLKNLGAGEKLIKRIFLFEGWM
                     ISTLGAVFGITLGIIVILLQQHFGFLKLGGDNYVVDAYPVVLLFSDILITFVSVLIMG
                     FLAALYPVKYISPGGVSK"
     gene            complement(1390..1746)
                     /gene="rbfA"
                     /locus_tag="PLN75_08995"
     CDS             complement(1390..1746)
                     /gene="rbfA"
                     /locus_tag="PLN75_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRG04291.1"
                     /translation="METTRQQKIARMLQKELSDIFLHYAREMQGTIISVTAVTVSPDL
                     SIARARLSIFPGDKATQVLEKINGDSRSIRFELGKRVRFQLRIIPELFFHIDDSLDYL
                     ENIDKLLNKDKETPDS"
     gene            complement(1845..>3065)
                     /gene="cysS"
                     /locus_tag="PLN75_09000"
     CDS             complement(1845..>3065)
                     /gene="cysS"
                     /locus_tag="PLN75_09000"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRG04292.1"
                     /translation="DKIGKKARLEELEPMEIVDYYTLRYHKAMEMLNVLPPSIEPKAS
                     GHIMEQIEFVEKILDAGYAYESDGSVYFDVEKYNKDHHYGILSGRNIDELLETTRNLD
                     GQSEKRRSVDFALWKKASPEHIMRWKAPWSVGFPGWHLECSAMGTKYLGEEFDIHGGG
                     MDLMFPHHECEIAQNQAGLGKSSVRYWMHNNMITIGGQKMGKSLGNFITLEQFFTGNH
                     PLLTKGFSPMTIRFFILQAQYRSTVDFSSEALEASEKGLERLIEGYARLQNLTAGEKS
                     SIEIGNLREKCEEAMCDDLNTPIVISHLFEYLRIINLAHDGKETLSQADLDELNSVFK
                     LFIEDILGLQSTETATEGIEAYRKAVDLLLNIRLEAKKNKDWATSDKIRNELTSYGFE
                     IKDTKDGFEWKVAP"
BASE COUNT          846 a          697 c          605 g          917 t
ORIGIN      
        1 cctatgggaa attaaaaagc tttcattcaa gcagatgtcc ttattcttat atcgaactca
       61 cattatttag aaacgccacc tggcgaaatg tatttaacgg gataaagcgc tgccagaaag
      121 cccataatca atacacttac aaacgtaata agaatatctg agaacaacaa aaccaccgga
      181 taagcatcca ctacataatt atcgcctcca agtttgagaa atccaaaatg ctgctgcaaa
      241 agaattacaa ttattcccaa agttatacca aaaacagcac caagagtaga aatcatccaa
      301 ccttcaaata gaaaaattcg ttttataagc ttttcgccgg ctccaaggtt tttcagaacg
      361 cggatatctt cttttttctc aagaataagc atggaaagcg atccgataat gttgaatgtg
      421 gcaataagca gtataaaaca aagaatgaga taagttatcc acttttccat tttcgtgatt
      481 ttgtaaaaat cttcctgctg ttcgtacctg tcgagtacct tgaagcgttc gccgagtatt
      541 tttcgaattt cagtcttcgt tttctgtatt tctgcagtcg gtttcacctt gatttcaaga
      601 cccgaaacgg tattttcatc gtattcaaac agttcgcggg ccaagcttag cgaagtgagc
      661 acatattgat catcgtattc gggctgattg accacaaata cacctgaaat atacgctgaa
      721 gcaggattaa agctgctttc cggtcgcacc atatttattc tgccggttct ttttggggca
      781 agaatttgca gtggattgat cacccgggca ttgattccca acttattggc tacccctatt
      841 ccaactacag cccgctggaa agctccgtcg taaagcacaa aacttccgtc gtacatgata
      901 ctgtcgatgc tggaaacttt gttaaaattt tcactgatac ccattattct ggcaggcatc
      961 tgtcggtcgg agaaactcag tagtgccgtt tcttcaatca cttcggcgta atgagccacc
     1021 gatccgagtt tcttaacctt aagtaaatca ggagtattca cattaaaggt tttaccttcg
     1081 acgagcgata ttttcaaatc cggatcaaat gcggagaaca tgccctgaat cagtgtgcca
     1141 aatccgttga aaaccgacaa tacacatata agggcagcgg taccgataga gactccggca
     1201 gccgaaatcc ccgaaatgac attgatggcg ttgtgagatt tttttgaaaa caaataacgc
     1261 ctggctaccc aaagggcaaa tttatggttg gtcataaact gtctgaacct gcctgcagca
     1321 ggcaggatat gattatagta tgatttcttt gattactttg atattgcgta tttgcatcaa
     1381 aaaatcccat taagagtcag gggtttcttt atctttattg aggagtttgt caatattttc
     1441 aagataatcc aggctgtcgt cgatatgaaa aaagagttcg ggaataatcc gtagttgaaa
     1501 ccgtacccgt tttcccaatt caaaccgaat ggatcttgaa tctccattga tcttctccaa
     1561 aacctgagtt gctttatcgc cgggaaatat gcttagcctt gccctggcaa tactcagatc
     1621 agggcttact gtgactgccg taacactgat aattgtaccc tgcatttcgc gtgcgtaatg
     1681 aagaaatatg tcactcagct ccttctgaag catacgcgct attttttgtt gtcttgttgt
     1741 ttccataaaa taagcccctc ctagcctccc caaaggggag ggaaaaaata ttattaaatt
     1801 ctataaaatt gaatagccct tctgtaagtc ccccttcggg ggatttaggg ggctaccttc
     1861 cactcaaatc catccttcgt atcttttatc tcaaagccgt aggaagtcag ttcattacgt
     1921 attttgtcgg atgttgccca gtctttgttc tttttggctt ccaggcggat attcagcagt
     1981 aaatcaacgg ctttacgata cgcttcgatg ccttcggtag ccgtttccgt gctttgtaag
     2041 ccaagaatat cttcgataaa tagtttgaaa acagaattta attcgtctaa atcggcttgc
     2101 gaaagcgttt cttttccatc gtgagcaaga ttgataatac gcagatattc aaatagatgt
     2161 gaaattacaa tcggcgtatt caggtcatca cacattgctt cttcgcactt ttcacgcaag
     2221 ttaccgattt ctatggaaga tttttcaccg gcagtcagat tttgaagtcg ggcgtagcct
     2281 tcaatcagtc gctccaagcc tttttcggat gcttccagag cttcactgct aaaatcaacc
     2341 gtgctacggt attgtgcttg cagaataaaa aagcggatgg tcatcgggct gaaacctttg
     2401 gtcagcaaag gatgatttcc ggtaaaaaac tgctccaacg tgatgaaatt tccgagcgat
     2461 tttcccattt tctgcccgcc gatagttatc atattgttgt gcatccagta gcgaacactg
     2521 cttttgccaa gtccggcttg attttgagcg atttcgcact cgtgatgcgg aaacatcaaa
     2581 tccatgcctc cgccgtgaat gtcaaattct tcgcccagat attttgttcc catagccgaa
     2641 cattctaagt gccaacccgg gaaacccacg ctccaaggcg ctttccaacg cataatatgc
     2701 tccggagatg cttttttcca aagtgcaaaa tccaccgaac ggcgtttctc actttgtccg
     2761 tcaaggttgc gcgtagtttc gagcagttcg tcgatattcc gcccggaaag tatgccgtaa
     2821 tggtggtctt tgttgtattt ttccacatcg aaatagaccg aaccatcgct ctcgtaagca
     2881 tatccggcat ccagaatttt ttcaacaaac tctatctgtt ccataatatg gccggacgct
     2941 ttcggctcga tgctcggagg aagcacattc agcatttcca tcgctttatg atagcgaagt
     3001 gtgtaataat caactatttc catcggttcg agttcttcga gtcgggcttt tttcccgatt
     3061 ttatc
//
LOCUS       DAORKP010000208         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9823_length_3058_cov_7.459207, whole genome shotgun sequence.
ACCESSION   DAORKP010000208 DAORKP010000000
VERSION     DAORKP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9823_length_3058_cov_7.459207"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLN75_09005"
     CDS             complement(<1..1209)
                     /locus_tag="PLN75_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRG04293.1"
                     /translation="MRIRLKNTLFLFAGMLIGILLMGCSGTKSLSDKNSVWVGSWSTA
                     PQLVEPHNMPPAPGLTDNTIRQIVRVSIGGDMVRLRFSNAFSKSPVEMKSVAIAQVSE
                     GSSIEISFQKLLKFKKNESVTMNPGGEVYSDPINFNLKSGSLIAITIHFGKTSPDVTG
                     HPGSRTTSFILPGNNISSADFSGSVKTDHWYVIQGIDVKASKNSKAVAILGNSITDGR
                     GSGTNKQNRWPDILSAKLLANESTKNIGVLNLGIGGNCVVRGGLGPTALERFDRDILS
                     QSNVRWLIILEGINDIGGIKSREEAEKVSTELIQAYQQMIDKAHSKGILVYGATILPF
                     AKSFYDTPERQIARDKVNDWIRSSGKFDAVIDFDKIMRNPNDVKTILSDMHDGDFLHP
                     NEAGYKRMGEN"
     gene            complement(1304..2866)
                     /locus_tag="PLN75_09010"
     CDS             complement(1304..2866)
                     /locus_tag="PLN75_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRG04294.1"
                     /translation="MKIKYLLFLLLLPGIFSYGQLRDAQVKVEGGIIQGVETDGMTVF
                     KGVPFAAPPVGELRWKAPQPVIGWEGIKKTVEFAPAPMQAGNPASGKSEDCLYLNIWT
                     PAKSSKDKLPVLVWIYGGGFSFGSTSDPVHNGENLAKKGVVVVSIAYRVGQLGFLAHP
                     ELSAENPNGVSGNYGLLDQIAGLKWVQNNIAAFGGDPHKVTIFGESAGGISVSMLCAS
                     PLTKGLFQGAISQSGGSFGPVRMTTYPGENMKTLSMAEADGVDYVKKMGVSSISDLRQ
                     LSAEKLIPQGWSMPGGWPIVDGYAIPDDQFALYEKGTYNDIPVLIGYNSDEGLSFPTG
                     RTKEEFIENVQKRFGPHAQALLKAYPLGENNIPRSARNLMRDAAFGWHTWSWARLQAK
                     TGNSKVYYYYFDQHAQQPKESPFYDYGTPHGVDVPFVFMNLDPNNGRTTESDLLISEI
                     MGTYWTNFAKYGNPNGNGLTNWPAFTNEKPVVMYLGPGAYLGKVPDEEALNVLDNYYT
                     WRRSAEGKEWAK"
BASE COUNT          847 a          709 c          594 g          908 t
ORIGIN      
        1 attttcaccc attcgcttgt atccggcttc atttgggtgg agaaaatcgc cgtcgtgcat
       61 atcggacaaa atggttttca cgtcgttcgg gttgcgcatt attttatcaa aatcaatcac
      121 agcatcaaat tttccggaac tccgaatcca atcgttgact ttatcccgtg caatctgccg
      181 ttcgggtgtg tcgtaaaaag attttgcaaa tggcaaaata gtcgctccgt aaacgaggat
      241 tcctttggag tgggctttgt caatcatctg ctgataggcc tggatcaatt cagtagaaac
      301 tttttcggct tcttctcgtg attttattcc tccgatgtcg ttgattcctt ccaggatgat
      361 cagccagcga acattgcttt gcgacaggat atcccgatca aaacgttcca gtgccgttgg
      421 tcccaaaccg ccacggacaa cgcagttgcc accgattcca aggtttaaaa ctccgatgtt
      481 tttggtagat tcatttgcta acagtttggc agaaagaata tccggccagc ggttttgctt
      541 attagtgccg gaacctctac cgtcggtaat tgaatttccc aaaattgcca ccgctttcga
      601 gtttttggat gctttcacat cgattccctg aataacatac cagtgatcgg tttttaccga
      661 gccggaaaaa tcagcagaag aaatattgtt tcccggcaaa ataaaggatg tggtacgtga
      721 gccgggatgt ccggtaacat caggcgaagt tttgccgaaa tggatggtaa tcgctatcaa
      781 agagcccgat ttaaggttaa aattaattgg atcagaatat acttctccgc cggggttcat
      841 cgttacactt tcgttttttt tgaatttcag caatttctga aaagatattt caatagaact
      901 tccttcagaa acctgagcaa ttgcaaccga cttcatttcc accggacttt tgctgaaggc
      961 attcgaaaaa cgcaatctga ccatatcacc gcctatcgaa acacggacaa tctgacggat
     1021 tgtattgtcg gttaatccgg gtgcaggagg catgttgtgc ggctccacca attgcggagc
     1081 cgtactccaa gaaccaaccc aaactgagtt tttatctgac aaggattttg taccggaaca
     1141 gcccatcaac aaaatgccga tgagcattcc ggcaaacaaa aacaacgtat tttttaatct
     1201 aattctcatt tttttctctt tttacaatga agaatggaaa acttcactgt ctggcatttt
     1261 tactatttga aaaaatacaa gcaaaacata tgctttcaag tatttatttt gcccattctt
     1321 tcccttctgc tgaccgcctc caagtatagt agttgtccaa cacattcagg gcttcttcat
     1381 caggaacttt tcccaaatac gccccgggtc ccagatacat cacaactggt ttttcatttg
     1441 taaatgcagg ccaattagtt aaaccattac cattgggatt gccatatttg gcaaaattag
     1501 tccaatatgt tcccatgatt tctgaaatca acaaatcaga ttcagtggtt cttccattat
     1561 tcggatctaa gttcatgaaa acaaacggca catcaacacc atgcggagtt ccataatcat
     1621 aaaaaggtga ctctttaggt tgttgggcat gttgatcaaa ataatagtaa taaacttttg
     1681 aatttccggt ttttgcttga aggcgcgccc aactccaggt gtgccaccca aatgcggcat
     1741 cacgcattaa gttgcgtgca gaacggggaa tgttgttttc acccaatgga taagctttca
     1801 aaagagcttg ggcatgagga ccaaaccttt tttgcacatt ttcaataaat tcctccttgg
     1861 tgcgtcctgt cgggaaactc agaccttcat ccgaattgta tccaattagt actggaatgt
     1921 cgttgtacgt tcccttttca taaagagcaa attgatcatc agggatagca tatccatcca
     1981 caataggcca tccgccaggc atgctccacc cttgcgggat aagtttttca gcactgagct
     2041 gacgtagatc actgatggat gatactccca ttttttttac ataatccaca ccatcagctt
     2101 ctgccattga taaagtcttc atgttttctc cgggatacgt agtcattcga acaggcccaa
     2161 atgagccgcc gctctgtgaa atagcacctt ggaataaacc ttttgtcaag ggcgaagcac
     2221 acagcatgct tacagaaata ccgccggcag actcaccgaa aattgtcact ttatggggat
     2281 cacctccaaa agcagcaatg ttattctgta cccatttcaa cccggcaatt tgatcgagca
     2341 ggccatagtt accggaaact ccatttggat tttctgcact caattcggga tgtgccaaaa
     2401 agccaagttg tccaacacga taagctatgc tgactacaac tactcctttt tttgccaaat
     2461 tttcaccatt gtgaaccgga tctgaggttg atccaaagct gaatcctcca ccataaatcc
     2521 agactaaaac cggtagttta tcttttgagg attttgccgg tgtccagatg ttcagatata
     2581 aacagtcttc actttttccg gaagcgggat tacctgcttg cataggtgcc ggagcaaatt
     2641 ccacggtctt ttttattcct tcccatccaa tgacgggctg aggagctttc cagcgaagtt
     2701 ctcccactgg cggggcagca aagggaactc ctttgaaaac agtcatgcca tcagtttcaa
     2761 ccccttgaat aattccacct tccactttaa cctgagcatc tcgaagttgt ccatacgaaa
     2821 agatgccggg taataaaaga aggaataaca aatattttat tttcatgtgt ttataattta
     2881 tatattacac taaggacaca tggaacacgc catcaatcat ctcattgtgt ttgagatttt
     2941 tctcaaatgg ctgtttcatc tgattataaa ttatttttta gtgtcatgtc tacttaaaca
     3001 gcaactgagc aaactgatac aaactcctgc gccagctctg ccattcgtga gctgtttt
//
LOCUS       DAORKP010000209         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_9998_length_3027_cov_9.573688, whole genome shotgun sequence.
ACCESSION   DAORKP010000209 DAORKP010000000
VERSION     DAORKP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9998_length_3027_cov_9.573688"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLN75_09015"
     CDS             <1..222
                     /locus_tag="PLN75_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRG04295.1"
                     /translation="TKQDSIEFVEDKIQQELALETAFEGNRFQDLMRFAIRRNDNAYL
                     ADKVASKHTANKEAIRNKLMTRENWYIRK"
     gene            465..1148
                     /locus_tag="PLN75_09020"
     CDS             465..1148
                     /locus_tag="PLN75_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRG04296.1"
                     /translation="MLELLKETVFNANLELVKHGLVIFTWGNVSAVDRDSNLIVIKPS
                     GVSYDDMKPEHMVVVDMDGHVVEGSFKPSSDTATHLALYKAFPNIGGVVHTHSTYATS
                     WAQAGLDIPNIGTTHADYFSDAIPCTRDMTQQEVEGEYEKETGTVIIERFSDINPDYV
                     PGVLVKNHGPFSWGKDAHDAVHNAVVMEQVAKMAFIACGVNPNLTMNEHLIKKHFFRK
                     HGPNAYYGQ"
     gene            1347..2852
                     /gene="araA"
                     /locus_tag="PLN75_09025"
     CDS             1347..2852
                     /gene="araA"
                     /locus_tag="PLN75_09025"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRG04297.1"
                     /translation="MINYNDFEVWFVTGAQLLYGGDAVVQVDAHSTEMVKGLNELGNL
                     PVKVVYKGTANSSAEISEIFRAANGDTKCVGVITWMHTFSPAKMWIHGLMDFKKPLLH
                     LHTQYNEKIPWETMDMDFMNLNQSAHGDREFGFITARLRKPRKIVVGYWKDKETHKKI
                     STWMRVSVAWADAQDMRIIRFGDNMNNVAVTDGDKVEAEIRLGYHVDNAPIAKLVPYV
                     NAVKDEEIDALVAEYEKEYDFAADAAKGGANHSHVRAAAAQEIGLRRFLEEKGAKAFT
                     SSFDELEGMDQLMGFASQRLMQDGYGFGAEGDWKTAALVRSMWVMGAGLEGGCSFLED
                     YTLNFEGENSSILQAHMLEINPSIAKKKPQLQVHFLGIGDAGTCARLVFQAHEGQGVA
                     CTIVDMGNRFRMIVNVVDVIETKDLPKLPVACALWIPQPNFEVGAGAWIQAGGTHHSS
                     FSFALTKEHMEDFAEIADMEMLLIDKDTTMRNFKQDLRNNEVYYMLNRALR"
BASE COUNT          973 a          623 c          692 g          739 t
ORIGIN      
        1 acaaaacagg attcaatcga atttgttgaa gataaaatcc agcaggaact ggcactcgaa
       61 acagcatttg aaggcaaccg ttttcaggat ctgatgcggt ttgccataag aagaaatgac
      121 aacgcgtatc tggctgataa agttgcatca aaacatactg ccaacaaaga ggcaatcaga
      181 aataaactga tgacaagaga aaactggtac atcagaaagt aataattcaa tcaaacgcag
      241 tcaggagagg gataatgagt ttcgttattc ctcttttttt tcgaaaattt atttatcaga
      301 actattcagg taatttattt aacttgttat tccacataaa acaacaaata tgtgttgcat
      361 aacataaaaa actaaaacga ttttcctgaa tattattaat atatttgtcc cgaattataa
      421 gtgtataatt tacacttaat aaacaaccaa taaaatatat tcagatgctg gaattattaa
      481 aagaaacagt ttttaatgcc aatctcgaat tggtaaaaca cggattggtt atctttacct
      541 ggggaaatgt cagtgcagtc gacagggatt cgaacctgat agtaatcaaa ccatccggcg
      601 tctcatacga tgacatgaaa ccggaacaca tggtagtggt ggatatggat ggacatgtag
      661 tggaaggcag tttcaagcct tcatcagaca cagccactca cctggctctt tacaaagcat
      721 tcccgaatat aggcggagtg gtgcacacac attccactta tgccacatcc tgggcacagg
      781 cagggctcga cattcctaac atcggaacaa cgcatgccga ttatttcagt gatgccatac
      841 cctgtacccg cgatatgacg cagcaggaag tggaaggaga atatgaaaag gaaaccggaa
      901 cggtcattat tgaaagattc agcgatatca atccggatta tgtacccgga gtactggtga
      961 aaaatcacgg tcctttttca tggggaaaag acgctcacga tgccgttcac aatgcagtgg
     1021 tcatggaaca ggtggccaaa atggctttca ttgcctgcgg tgtgaatcca aatctgacca
     1081 tgaacgagca cctgatcaaa aagcatttct tccgcaagca cggaccgaat gcctattacg
     1141 gacagtaaaa gaaagcccct ctaattctca ccgaagggga gattttttag aaacgtgaac
     1201 gaaagtgcag tgaagcagta gtagttggtg gaaagtgctg cctcacttaa cagccaatat
     1261 cagtttttgt aaaagcaaac aaaattaatt attaacaaac cttatttctc aaaaaagtaa
     1321 ataaggataa aaaaatttga aagaaaatga taaattacaa tgatttcgaa gtatggttcg
     1381 ttaccggagc tcagcttctt tacggtggcg acgcagtagt gcaggtggat gcacactcaa
     1441 ccgaaatggt aaaaggattg aacgaactgg gaaatctccc cgtaaaggtg gtttataaag
     1501 ggactgctaa ttcctcggct gaaatttcag aaattttccg cgctgccaat ggcgatacca
     1561 aatgtgtggg tgtaatcacc tggatgcaca cattctcacc tgctaaaatg tggatacatg
     1621 gtttgatgga cttcaaaaaa ccattattac accttcatac gcaatacaat gaaaaaattc
     1681 cgtgggaaac aatggatatg gacttcatga acctgaacca gagcgcacac ggcgatcgcg
     1741 aatttggttt catcacagcc cgcctgcgca aaccccgcaa aatcgttgtc ggatactgga
     1801 aagacaagga aactcataag aaaatctcta cctggatgcg cgtatcagta gcctgggccg
     1861 atgctcagga tatgcgaatc atccgtttcg gcgacaatat gaacaacgtg gcggtaactg
     1921 acggtgataa agtggaagct gaaatccgcc tgggatacca cgtggataat gctcctatcg
     1981 ccaaattggt tccttatgta aatgcagtga aagacgaaga aattgacgca ctggtggcag
     2041 aatacgaaaa agaatacgac tttgctgccg atgcagccaa aggtggtgca aatcacagcc
     2101 acgttcgtgc agctgctgct caggaaatag gtctgcgccg ttttctcgaa gaaaaagggg
     2161 caaaagcatt tacatccagt ttcgacgaac tcgaaggaat ggatcagctg atgggatttg
     2221 cttctcagcg cctgatgcag gatggctatg gatttggtgc cgaaggcgac tggaaaacag
     2281 cagcgctggt tcgctccatg tgggtaatgg gagccggtct cgaaggcggt tgctcattcc
     2341 tcgaagatta cacgttgaat tttgaaggtg aaaacagctc aattcttcag gcacacatgc
     2401 tcgaaatcaa tccatctatt gctaagaaaa agcctcagct tcaggtacat ttcctcggta
     2461 tcggcgatgc cggaacctgt gcacgtctgg tattccaggc tcacgaaggt cagggagttg
     2521 cctgcaccat cgtagatatg ggaaatcgtt tccgcatgat tgtaaatgta gtggatgtaa
     2581 ttgaaacgaa agacttaccg aaacttccgg tggcatgtgc attgtggatt ccgcaaccaa
     2641 acttcgaagt gggtgcaggt gcatggatac aagccggtgg tactcaccac tccagtttct
     2701 cttttgccct caccaaagaa cacatggaag actttgccga aatagcagac atggaaatgc
     2761 tccttatcga caaagatacc accatgcgca atttcaaaca ggacctgcgc aacaacgaag
     2821 tgtattatat gcttaacaga gcgttgagat aaaaaaaagc ccctccaacc tccccgaagg
     2881 ggaggctttg ggtgggagca tcaatttaga atagtaagta attaatagaa atatctaaaa
     2941 attcttcccc ttcggggaag ttagaagggg ctttatatga aatacgtaat aggtttagac
     3001 tacggaagcg attcggccag agcagtc
//
LOCUS       DAORKP010000210         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_10157_length_3000_cov_7.747708, whole genome shotgun sequence.
ACCESSION   DAORKP010000210 DAORKP010000000
VERSION     DAORKP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10157_length_3000_cov_7.747708"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLN75_09030"
     CDS             complement(<1..353)
                     /locus_tag="PLN75_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRG04298.1"
                     /translation="MDTTLRDGEQTSSVSFSSQEKLSIARLLLEDLGVDRIEVASARV
                     SDGEFNSVQKIAQWAATHGFIDRVEVLGFVDGTASLEWIQKTGCRVINLLCKGSLNHC
                     THQLKKTAQEHLADI"
     gene            complement(491..832)
                     /locus_tag="PLN75_09035"
     CDS             complement(491..832)
                     /locus_tag="PLN75_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRG04299.1"
                     /translation="MNIEDLREYCISLPEVEECFPFDEVTLVMKTGGKMFALIPLDEV
                     ETCISVKCNPELAIELRETYSCVEPAFHMNKTHWNTIFISQEITEKQIKEWIKHSYDL
                     VKAKLPKIKHP"
     gene            complement(903..1493)
                     /gene="leuD"
                     /locus_tag="PLN75_09040"
     CDS             complement(903..1493)
                     /gene="leuD"
                     /locus_tag="PLN75_09040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRG04300.1"
                     /translation="MEKFKTIKSSVVPLPIENVDTDQIIPARFLKATDKNGFGDNLFA
                     DWRYNKDGSPKTDFVLNNPVYSGEILVAGKNFGSGSSREHAAWAIAGYGFKVVVSSFF
                     ADIFKNNALNNGILPVVVSSEFLAEIFASVNENPKTTLTVDLVNQTISNNTSGKSETF
                     EINAYKKDCLLNGFDDIDYLLSKKALIEGWEKKNTR"
     gene            complement(1546..1920)
                     /locus_tag="PLN75_09045"
     CDS             complement(1546..1920)
                     /locus_tag="PLN75_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRG04301.1"
                     /translation="MLHQELTEEIISAFYKVYNTLGYGFLESVYKNAMFFELLDSELE
                     CRTEQPVDVYYERRMVGKFFVDILVENKVILELKSAECIREEHEAQLLNYLKATNIEI
                     GLLLNFGKKPEFKRFIYSNNRK"
     gene            complement(2032..>3000)
                     /locus_tag="PLN75_09050"
     CDS             complement(2032..>3000)
                     /locus_tag="PLN75_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRG04302.1"
                     /translation="IAFGIGTSEVEMVLATQCILQSRPKTMRITFDGNLNNGVTSKDL
                     ALYMISKLTTGGATGYFVEYAGEAVRNMTMEQRMTVCNLSIEMGARGGLIAPDETTFN
                     YVKGREFAPKGEEWEEKLAYWKTLKSDDDAVFDMELTFDASEIQPMITYGTNPGMGMG
                     IHESIPTLDEIDEAGRISFRKSLEYMGFEPGEKLLGKSIDYVFLGSCTNGRIEDFRLF
                     ANYVKGRKKAENVTAWLVPGSWKVRQQIEAEGLDKILEEAGFALRQPGCSACLAMNDD
                     KVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVNGKITMP"
BASE COUNT          751 a          708 c          604 g          937 t
ORIGIN      
        1 ctgatgtcag caaggtgttc ctgtgccgtt tttttcaact gatgagtgca gtggttcagc
       61 gaacctttgc aaagcagatt aatcacccgg catcctgttt tctgaatcca ttccagcgaa
      121 gctgtgccat cgacaaagcc aagcacttcc acccgatcga tgaaaccgtg tgtagcagcc
      181 cactgcgcaa ttttctgtac ggagttgaat tcaccatccg aaacgcgtgc agatgccact
      241 tcgatgcggt ctactcctaa atcttccagt aaaaggcgag caatactcaa tttttcttgc
      301 gaagagaatg ataccgatga agtttgctcg ccgtcgcgaa gcgtagtatc catgatttcg
      361 acgaagcctc gccctgtcgg cttgagaggc ggattttggt gtggttgttc aggtttattg
      421 agtttatcta tattttccat tttttcaaaa attcgatgat acatattgta ctattattcc
      481 cctacaggag ttaagggtgt tttattttag gtaatttcgc ttttaccaaa tcatacgaat
      541 gttttatcca ttctttaatc tgtttttcgg taatttcctg tgaaatgaaa atcgtgttcc
      601 agtgtgtctt gttcatgtgg aatgccggct caacgcaact gtatgtttct ctcagctcta
      661 tcgccagttc gggattgcac ttcaccgaaa tacacgtttc tacctcatcg agcggaatca
      721 aagcaaacat ctttccaccg gttttcataa ccagtgttac ctcgtcgaag gggaaacact
      781 cttcaacttc cggaagcgaa atgcagtatt ctcttaaatc ttctatgttc acgaataaca
      841 cgaattaatg cgaatttcac taaataaacg aaccctcctg tggcaggcag gttacacgaa
      901 ttttaacggg tatttttctt ctcccagcct tcaatcaatg cttttttgct cagcagataa
      961 tcgatatcat caaatccgtt aagcaggcag tcttttttgt aagcgttgat ttcaaatgtt
     1021 tccgattttc cgctggtgtt gttgctgatg gtttggttca ccaaatccac agtcagcgtt
     1081 gttttcggat tttcgttgac cgaagcgaag atttctgcca aaaattcgga tgaaaccacc
     1141 accggaagta tgccgttgtt cagggcgttg tttttgaaaa tatcggcaaa gaagctcgaa
     1201 accaccactt taaagccata tcctgcaata gcccatgcag catgttcgcg gctcgaaccc
     1261 gaaccaaagt ttttcccggc aaccagtatc tcacccgagt aaacaggatt gttgagcacg
     1321 aaatctgttt ttggacttcc gtctttattg tatcgccagt cggcaaagag attgtcgccg
     1381 aagccgtttt tatcggtcgc tttcaggaaa cgcgccggaa ttatctggtc ggtatccaca
     1441 ttctctatcg gaagcggaac gacactgctt tttattgttt taaatttttc cattttatga
     1501 tatagtacgc ggataacacg gattaaacgg atttccgctg attttttatt ttctgttgtt
     1561 gctgtatata aaacgcttga attcaggctt tttgccaaaa ttcaacaaaa gtcctatttc
     1621 aatattcgtt gcttttaaat agttaagtaa ttgagcctca tgctcttcac gtatgcattc
     1681 tgccgatttc agttcaagga taactttgtt ttcaacaaga atatccacga aaaattttcc
     1741 aaccattctc cgttcataat aaacatcaac cggttgttcc gttctacatt ccaactcaga
     1801 gtccaacaac tcaaaaaaca tggcattttt atatacactt tctaaaaatc catatcccag
     1861 cgtgttgtaa actttgtaaa atgcagaaat aatttcttcg gttaattcct ggtgtaacat
     1921 tttgtttttt taatttgcgt aaatccgcta aatccgtgtt atctgtgtac tgataaatat
     1981 ttaaagcctt tttttgcacc caatattccc catccagggg gttagggggc tttacggcat
     2041 tgtgattttt ccattcactg cagctgcggc agccaccagc ggccctgcca ggatggtgcg
     2101 tgcacccgga ccctgacgac cttcgaagtt gcggttggag gtgctgacgg catatttgcc
     2161 ggcaggaacc ttgtcgtcgt tcatggccag gcaagctgag cagcccggct gacgaagagc
     2221 gaatcctgct tcttcgagga ttttatccag accttctgct tcgatttgct gacgcacttt
     2281 ccagctaccg ggcacaagcc atgctgttac gttttcggct tttttcctgc ctttcacgta
     2341 gttggcaaaa aggcggaaat cttcaattcg accattggtg caactgccca gaaatacata
     2401 atcaatcgat tttccaagca gtttttcgcc gggttcgaaa cccatgtatt ccagtgattt
     2461 ccggaaagaa atgcgtcccg cttcatcaat ttcatccagc gtaggaatgc tttcatggat
     2521 gcccattccc ataccgggat tggtaccgta agtgatcatc ggttgaattt cagatgcatc
     2581 gaaagtcagt tccatatcaa aaactgcatc atcatccgat ttcagcgttt tccaataggc
     2641 cagtttttcc tcccattctt cgcctttcgg tgcaaattcg cgccctttta cgtagttgaa
     2701 agtcgtttca tccggtgcaa tcaaaccacc gcgggcaccc atttcgatag aaaggttgca
     2761 aactgtcatc cgttgttcca tagtcatatt gcgaaccgct tctccggcat attccacaaa
     2821 ataacccgta gcaccgcctg ttgtcagttt tgaaatcata taaagtgcta aatccttgga
     2881 agttacacca ttgttcagat taccgtcgaa ggtaatgcgc attgttttag gccggctttg
     2941 caggatgcat tgcgtagcaa gcaccatttc cacttcgctg gtgccgatgc cgaatgctat
//
LOCUS       DAORKP010000211         2952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_10471_length_2952_cov_10.255782, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000211 DAORKP010000000
VERSION     DAORKP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10471_length_2952_cov_10.255782"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLN75_09055"
     CDS             complement(<1..988)
                     /locus_tag="PLN75_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HRG04303.1"
                     /translation="MRKTFLFFAIILLGYSSGASAQFKSGEFKNWAKTPPMGWNSWDC
                     YGPTVEEHEVKSNADYMAKHLKKYGWEYIVVDIRWFVENDKAGGYNQTDPRYVLDEYG
                     RYQPAVNRFPSAKDGKGFKELADYVHKKGLKFGIHIMRGIPKEAVAKKLPIKGTNGIT
                     ADKIYTDSIQCTWLRDNYTILDVPGAQEYYNSIFELYASWGVDFIKIDDLSRPYHQTE
                     IEMIRKAIDRCGRKIVLSTSPGETPIDKADHVKDHANMWRMVDDVWDEWRHITHLMKI
                     SEKWYDYIEPTWPDCDMIPLGRISIRGERGADRMSRLTKDEQYTLMNLFTIFK"
     gene            complement(988..>2952)
                     /locus_tag="PLN75_09060"
     CDS             complement(988..>2952)
                     /locus_tag="PLN75_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRG04304.1"
                     /translation="YLLDKEESVYGLGQQQVDNLNQRNTSHFMRQRALYASVPIIQST
                     KGYGMFWDNYSPTTYTDNPQEMSFNSEVGECIDYYFMYGGNADGVIAQIRDLTGQSPM
                     FPLWTFGFWQSRERYKSQWETMEVVDKYRELGVPLDGIIQDWQYWGPNTNWNSMNFDN
                     PEFPEPQKMIDHIHKNNAKIMISIWASFGPDTRQYNELKAKDGLLEFTTWPESALQTW
                     PPDRTKPSGVRVYDAYNEKARDIYWNHLNKGLFSKGIDAWWTDSTEPDHLNVQEKDFD
                     IATAMGTYRSVVNAFPLMTNSGIYDRQRAVSSDKRVYLLTRCAFAGQQRYGANTWSGD
                     VVSNWNVFKKQIPTGLNFSLTGIPYWNTDIGGFFHWDYEGEATNKAFHELYVRWFQYG
                     TFLPMLRSHGSGVKKEIYNLGKKGDWVYDTEEKYINLRYALLPYLYSTGWQVTKNSGS
                     FLRALFMDFNDDKKVHAINNQYMFGKAFLVTPVTEPMYVFSKENKWRNPYEDFSKTGK
                     QEVYLPKGTKWIDFWTGETYNGGQTVVKDVPIDIIPLYVRAGSIVPFGPKVQYATEKK
                     WDNLELRIYPGDDGDFTLYEDENDNYNYEKGAFSEIPISWSEATKTLTIGTRKGAFKG
                     MLKNRKFTVKRIDGSIKTVSYKGKEIKVKL"
BASE COUNT          761 a          690 c          570 g          931 t
ORIGIN      
        1 atttgaaaat ggtaaacaga ttcatcagtg tgtactgctc atctttcgtc agtcgggaca
       61 tccggtcggc gcctctttct ccacgaatgg aaatccttcc cagcggaatc atatcgcaat
      121 caggccaggt cggttcgata taatcgtacc atttttcgga aattttcatc aaatgggtga
      181 tatgtcgcca ttcgtcccaa acgtcgtcca ccatgcgcca catattggca tgatccttca
      241 cgtggtctgc cttgtcgatg ggagtttcac ccggtgatgt actcaaaacg atttttctcc
      301 cgcaccggtc aattgccttg cggatcattt ctatttcagt ctggtggtac gggcgggaaa
      361 ggtcgtcaat cttgataaaa tccactcccc aagacgcata cagttcaaat atagaattgt
      421 aatattcctg cgctcccggc acgtccagaa tggtgtaatt atctcttaac caagtgcact
      481 gaatcgaatc ggtgtagatt ttatcggccg taattccgtt tgtacccttg attggaagct
      541 ttttagcaac agcttccttc ggaatgccac gcataatgtg aattccgaat ttcagccctt
      601 ttttatgcac ataatcggcc aattctttga atcctttacc gtcttttgct gaggggaaac
      661 gattcacggc cggctgataa cgtccgtatt catccagcac atagcgagga tcggtttggt
      721 tgtatccgcc ggctttgtca ttctccacaa accaccgaat atccaccaca atgtattccc
      781 aaccgtattt tttcaaatgc tttgccatat aatcggcatt gcttttcact tcgtgctctt
      841 ccacagtcgg gccgtagcaa tcccagctgt tccaacccat cggcggagtt tttgcccagt
      901 ttttgaattc tccggactta aattgtgctg atgcacccga actgtatccg agcagaataa
      961 ttgcaaaaaa taaaaaagtt tttctcatta tagttttact tttatttctt ttcctttgta
     1021 tgaaactgtt tttatagaac cgtcgattcg cttaacggta aattttcgat ttttcagcat
     1081 tcctttgaaa gctccttttc ttgttccgat tgtcaatgtt ttggttgctt cactccacga
     1141 aattggaatt tcggaaaaag cgcccttttc atagttgtaa ttgtcatttt catcttcata
     1201 aagcgtgaaa tcaccatcat ctcccggata aattcttagc tccagattgt cccatttttt
     1261 ctcggtagcg tattgcactt tcgggccaaa tggaacgata gatccggcac gaacatacaa
     1321 tggaataata tcaatcggca catctttcac tacagtttgt ccgccattgt aggtttctcc
     1381 tgtccagaaa tcaatccatt ttgttccctt tggcagataa acttcctgct ttccggtttt
     1441 gctgaaatct tcatagggat ttctccattt gttttccttt gaaaacacat acatcggttc
     1501 ggtaacaggc gttaccagaa atgcttttcc aaacatatac tgattattta tggcatgaac
     1561 tttcttatca tcattaaaat ccataaacaa tgcacggagg aatgagcctg aatttttggt
     1621 aacctgccaa cctgtggagt acagataagg gagcaaggcg tagcgaagat ttatgtattt
     1681 ttcctctgta tcgtacaccc agtcgccttt tttacccaaa ttgtatattt cttttttaac
     1741 tccactgccg tgcgaacgaa gcataggaag aaacgttccg tattgaaacc aacggacgta
     1801 gagttcgtga aatgctttat tagttgcttc accttcataa tcccaatgga aaaagcctcc
     1861 aatgtcagta ttccagtaag gaattccggt tagagagaaa ttcaatccgg ttgggatttg
     1921 ttttttgaac acattccagt tggaaaccac atcacccgac caggtattgg ctccgtatcg
     1981 ttgctgtccg gcaaatgcac atcgggtaag taaatacact cgtttatcgg aagaaacggc
     2041 acgctgacgg tcgtaaattc cactgtttgt cattagcgga aatgcattaa ccacgctacg
     2101 atacgttccc attgcagtag caatatcaaa atccttttcc tgcacattca aatgatccgg
     2161 ttcagtggaa tctgtccacc atgcatctat tccttttgaa aataatcctt tgttcaaatg
     2221 attccagtaa atatcgcggg ctttttcgtt gtaagcatcg taaaccctta cgccagaggg
     2281 ctttgttctg tcgggtggcc aggtttgaag cgctgattcc ggccaggtgg tgaattccag
     2341 caatccgtct tttgccttta attcgttgta ttgcctggta tccggaccaa atgatgccca
     2401 gattgaaatc ataattttgg cattattctt atgaatgtgg tcaatcattt tttgtggttc
     2461 agggaattcc ggattatcaa aattcatcga attccaattg gtatttggtc cccaatactg
     2521 ccaatcctga atgattccat caagaggaac gcccaattca cgatatttat ctacaacttc
     2581 cattgtttcc cattgcgact tgtaacgttc gcgcgactgc caaaaaccaa aagtccagag
     2641 cggaaacata ggtgactggc cggtcaaatc acggatttga gcaatcactc catcggcatt
     2701 tccaccgtac ataaaataat agtctatgca ttcgcccact tcagaattga atgacatttc
     2761 ctgcggatta tccgtgtatg tggtcggtga ataattatcc caaaacattc cgtatccttt
     2821 tgttgactga ataatgggta cactggcgta caaggctctc tgacgcataa aatgcgaagt
     2881 gtttcgctga ttcaggttgt ccacctgttg ctgtccaagt ccataaacgg actcttcctt
     2941 atccaaaaga ta
//
LOCUS       DAORKP010000212         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_11184_length_2846_cov_9.526335, whole genome shotgun sequence.
ACCESSION   DAORKP010000212 DAORKP010000000
VERSION     DAORKP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11184_length_2846_cov_9.526335"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2846
                     /locus_tag="PLN75_09065"
     CDS             <1..>2846
                     /locus_tag="PLN75_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRG04305.1"
                     /translation="KYDAFALEEDGRVDEQIFNEYVSLNLRYPPRFTLVGSEEEANAA
                     LNERTYELIISMPSGDSINPFEWAKKVKEKHNDIPIVVLTPFSKMVSERISNEDLSAI
                     DYVFSWLGNSDLLLAIVKLIEDRMNVEEDVNSVGVQVIMLVEDSIRFYSSLLPHLYKY
                     VFIQSRSFMTEALNEHEQMLRMRGRPKILMARSYEEAMTIYSRYKKNMLGVITDVSYT
                     KNGVKNQLAGIELCQEIRKQDSYLPLIVQSTDVRNHPYADSVKAAFVHKLSKTPMLEL
                     RDIITNIFGFGDFVFINPQTGDEEARIGNLRGLQEIIFKVSDDSLYHHISQNNISRWL
                     YSRAMFPLAEFLKNIKVDTQEKTDLPRVRQIIFDAIVTYRKIKNRGVVAIFQRDRFDQ
                     YSNFARIGEGSLGGKGRGLAFIDSMIKTNDILDNFKDTEIMIPRTVVLCTDIFTEFME
                     KNDLYPIALSDVEDEVILKHFLKARLPQNIVADLYAFLGAIDSPVAIRSSSLLEDSHY
                     QPFAGIYSTYMVPHDKTSKTNMLMMITEAVKAVYASVFYQASKSYMKATKNVIDEEKM
                     AIVLQETCGNYYGTRFYPSFSGVARSLNFYPLGAEKAEEGIVNVAMGLGKYIVDGGMT
                     LRFSPHHPNNVLQTSTLDMALRETQTYFYALETSRATFVPQVNDSFNLMKVRVQDAES
                     DGTLKFLASTFNPQDQRIYDNIYEKGRKVITFANILQHNVFPLANILQEILRISQSEM
                     GRPIEIEFAVCLDYSPKPSHKFYLLQIRPIVDSKETINEDIGAIPDTHTIISCQNALG
                     HGINNEIFDLVYVRPEAFNASKNQLIMYDIEKVNKNLQKEERHYVLIGPGRWGSSDTW
                     LGIPVKWPHICNARVIVESGLSNYRIDPSQGTHFFQNLTSFGVSYFTINPFQKDGSYD
                     IDYLNELPAVYESEYLRHVRFEKPIVIKVDGRKN"
BASE COUNT          821 a          629 c          682 g          714 t
ORIGIN      
        1 aagtatgatg catttgcact ggaggaagac ggacgggtgg atgagcagat tttcaacgaa
       61 tacgtatcgc tcaacctgcg atatccgccg cggtttacgc tggtgggaag tgaagaggaa
      121 gccaatgcgg ctctcaacga acgcacgtac gaattgatta tctccatgcc cagcggcgac
      181 agcatcaatc catttgaatg ggctaaaaaa gtaaaggaaa aacacaacga catacccatc
      241 gtagtactca ctccgttttc aaaaatggta tcggagcgca tcagcaacga agacctgagt
      301 gctattgatt atgtattcag ctggcttggc aattcggatt tgctgctggc catagtgaaa
      361 ctgatagaag accggatgaa tgtggaggaa gatgtaaact cggtaggtgt acaggtcatc
      421 atgctggtgg aagattccat caggttctat tcatcgcttc tgccacacct gtacaagtac
      481 gtgtttatcc agtcgcgatc gtttatgact gaagcgctca acgagcacga gcaaatgctc
      541 cggatgcgcg ggcggccaaa aattctgatg gctcgcagct atgaagaggc catgacgata
      601 tactccagat acaaaaaaaa catgctcgga gtaatcaccg atgtaagcta tacgaaaaac
      661 ggagtgaaga atcagcttgc aggcattgaa ctttgtcagg aaatcaggaa acaggattcc
      721 tatctgccgc tgatcgttca gtcaaccgat gtgcggaatc atccatacgc cgattcggta
      781 aaagcagcat ttgtacataa attatccaaa accccgatgc tggaactccg cgacatcatt
      841 acaaatattt ttggttttgg cgattttgtt ttcatcaatc cccaaacagg cgatgaagaa
      901 gcccggatag gaaatctccg gggtttacag gaaatcattt tcaaagtgag cgacgactcg
      961 ctttaccatc atatatcgca aaacaacata tcgcgctggc tgtattcacg ggctatgttt
     1021 ccgcttgcgg aatttctgaa aaacatcaag gttgatacgc aggaaaaaac cgatctgccg
     1081 cgggttcgtc agattatttt tgatgccatc gtaacgtatc ggaaaattaa aaacaggggt
     1141 gtggtagcca tttttcagcg cgacaggttc gaccagtatt ccaattttgc ccgtataggc
     1201 gagggttcgc tcggcggaaa agggcgcggt ctggccttca ttgactcgat gattaagacc
     1261 aacgacatcc tggacaattt caaggatacg gaaataatga taccgcgcac agtggttttg
     1321 tgtactgata ttttcaccga attcatggag aaaaatgatt tgtatcccat tgcgctgtcg
     1381 gatgtggaag atgaggtaat tttgaagcat tttctaaaag cccgcctgcc tcagaatatt
     1441 gtggccgatt tatacgcttt tctgggtgcc attgattcac ccgttgccat ccgctcatcg
     1501 agcctgctcg aagattctca ctatcagcca tttgcgggca tttattctac ctacatggtg
     1561 cctcacgaca agacgagtaa aaccaatatg ctgatgatga ttaccgaggc ggtgaaagcg
     1621 gtgtatgcct cggtattcta tcaggcaagc aagtcatata tgaaagccac gaaaaacgtg
     1681 attgatgaag aaaagatggc tatcgtgctg caggaaacgt gcggaaatta ttacggcacc
     1741 cgcttttatc cgtcgttttc aggggtggca cggtcgctga atttttaccc gctgggtgcc
     1801 gagaaagccg aagaaggcat tgtgaatgtg gcgatgggat tgggcaaata cattgttgac
     1861 ggcggtatga cgctgcgatt ttcacctcat catcccaaca acgtgctgca aaccagtacc
     1921 ctcgatatgg ctttacgcga aactcagacc tatttctatg cgctggaaac gagccgggcc
     1981 accttcgtac ctcaggtaaa cgatagtttc aacctgatga aagtacgggt acaggatgcc
     2041 gagagcgacg gtacgcttaa gtttctggca tccacattca atcctcagga tcagcgcatc
     2101 tacgataata tttacgaaaa agggagaaaa gtaatcactt ttgccaatat tctacagcat
     2161 aatgttttcc cgctggctaa tatactccag gagattttac gcatttcgca gtcggaaatg
     2221 ggacgaccga ttgaaattga atttgccgta tgcctggatt attcacccaa accaagtcat
     2281 aaattttatc tgctgcagat cagacctatc gttgacagta aagaaacgat aaatgaagac
     2341 attggagcca taccggatac gcatacaata atcagttgcc agaatgcact ggggcatggt
     2401 attaacaatg aaattttcga tttggtgtac gtgcgtcccg aggctttcaa tgcatcgaaa
     2461 aatcagctaa tcatgtacga tatcgaaaaa gtgaataaaa atctgcaaaa agaagaacgt
     2521 cactacgtgc tcatcggtcc gggcagatgg ggctcgagcg acacgtggct cggtataccg
     2581 gtaaaatggc cgcatatttg taacgcccgg gtgatagtag aaagcggact gtccaactac
     2641 cgcatcgatc cgagtcaggg cacacacttt ttccagaacc tgacttcttt cggcgtttcg
     2701 tatttcacca tcaatccatt tcagaaagac ggcagctacg atattgatta tctgaatgag
     2761 ctgcccgccg tttacgaatc tgaatatctg aggcatgtcc gattcgaaaa acccatagtt
     2821 attaaagtgg atggccggaa aaatga
//
LOCUS       DAORKP010000213         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_11935_length_2745_cov_8.368401, whole genome shotgun sequence.
ACCESSION   DAORKP010000213 DAORKP010000000
VERSION     DAORKP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_2745_cov_8.368401"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLN75_09070"
     CDS             <1..443
                     /locus_tag="PLN75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRG04306.1"
                     /translation="KLGFTNVEFIQGDIEEMPLPDNAFDVVISNCVLNLVPDKQKAFR
                     EIFRVLKPGGHFCVSDVVIKGTLPDKLQKDAEMYAGCVSGASNMDDYLEIIHNQGFKN
                     IVIHKEKVISIPENVLDNYLSKEEIVSLTNGDTGIISVTVSAKK"
     gene            449..1513
                     /gene="arsB"
                     /locus_tag="PLN75_09075"
     CDS             449..1513
                     /gene="arsB"
                     /locus_tag="PLN75_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRG04307.1"
                     /translation="MPSKKRLKFTDRYLTLWIFLAMFIGVFSGWMFPAVAQFWNSMSS
                     GTTNIPIAIGLIVMMYPPLAKVKYEELGEVFKNTRVLGLSLVQNWIIGPVLMFLLAVL
                     LFKFFPGENNANLPYVYGIIMIGLARCIAMVIVWNDLAKGDTQYAAGLVAFNSIFQVL
                     FFSVYAYFFIAIAPGWFGIPTSDAVAKITIGQIAESVFIYLGIPFIAGFLTRFILIRV
                     RSKEWYHTKFIPKISPLTLIALLFTIIVMFSLKGEYIVKIPMDVLLIAVPLLIYFVIM
                     FVGSFWMSKKAGATYEQSTTLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVE
                     VPVMIALVNVAFWFRKKWFA"
     gene            complement(1615..>2745)
                     /locus_tag="PLN75_09080"
     CDS             complement(1615..>2745)
                     /locus_tag="PLN75_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04308.1"
                     /translation="ETDIYSQRGWYDTKNDRMMLLNRSLVEQNDGKTLIGDTIFYDKK
                     KKYGEAFSDVELTDPKQKSTLTGNFVSYDEISRVGLATDSALLIDWSTRDSLFMSADT
                     LISMRDSVATDSVSFNMVKAFKNVRFLRSDIQGMCDSLLYTSRDSILHLRVLPVLWSD
                     NNQLMGNKMTAYTKNQKVEKVHIEQSGIAIQKDSLNYFNQLSGKEITAYLDSGQLSKV
                     YVNGNAETIYFPKDDKTGEYIGINKTLSSYVTMYFKDKKVQRIVLTTASSGAMYPISQ
                     MEENDLYLRNFFWYETERPLKVDDVFTKYVRTEPPKRQESTKRPSFPGESSGNSTDAA
                     SKTNRPSGTTNRTNTNNSMGPVNSGGNMNPLNNLNRRGMIQQ"
BASE COUNT          699 a          542 c          625 g          879 t
ORIGIN      
        1 ccaaacttgg gtttacgaat gtggaattca ttcagggtga tattgaggaa atgcctttgc
       61 ccgacaatgc ctttgatgtg gtgatttcga actgcgtact taatctggtg ccagataagc
      121 aaaaggcttt tcgtgagatt tttcgtgtgc ttaagccggg agggcatttc tgtgtatcgg
      181 acgtggtaat taaaggcact ttacccgata aattacagaa ggatgccgaa atgtatgcgg
      241 ggtgtgtctc cggtgcaagc aatatggatg actatttgga gataatacac aatcagggat
      301 ttaagaatat tgttattcac aaagaaaaag tgatttctat tccggaaaat gttctggata
      361 actatctgtc aaaagaagaa atcgtaagtt taacaaatgg agatacagga atcataagcg
      421 ttactgtttc agcaaaaaaa taaaaagtat gccatctaaa aaaagattaa aatttaccga
      481 ccgctatctc acactctgga tttttctggc catgtttatc ggtgtgtttt cggggtggat
      541 gtttcctgct gtggctcagt tttggaatag tatgtcatcg ggcacaacca atatcccgat
      601 agctatagga ctaatagtga tgatgtatcc gccgttggca aaagtgaaat acgaagaact
      661 gggcgaagtg tttaagaata caagggtgct gggactgtcg ctggttcaaa actggattat
      721 cggaccggta ctgatgtttc tgctggctgt tttgcttttc aaattttttc ccggagaaaa
      781 taatgccaac ctaccgtatg tgtatggtat tatcatgatc gggctggcgc gttgtattgc
      841 tatggtcata gtttggaacg atctggcaaa aggagacaca caatatgcag ccggattggt
      901 ggctttcaac agtatttttc aggtgctttt cttctcggtg tatgcttact tttttatcgc
      961 cattgctccg ggttggtttg gaattcctac cagcgatgct gtagctaaaa tcaccatcgg
     1021 acaaattgcc gaaagcgttt tcatttacct cggtattccg ttcattgccg gattcctcac
     1081 ccgttttatc cttatccggg tgagaagcaa agagtggtat cacaccaaat ttattccgaa
     1141 aatcagtccg ctgacactca ttgcgctgct tttcaccatt atagtgatgt tctcgcttaa
     1201 aggcgaatac attgtaaaaa tacctatgga cgtattactg atagctgttc cattgcttat
     1261 ctatttcgtc atcatgtttg tgggttcatt ctggatgagc aaaaaggcgg gagcaactta
     1321 cgaacaatca accacgctgt cgtttacagc tgccagcaat aattttgaac tcgccattgc
     1381 cgttgccatt gccgtttttg gcatcaattc cggcgaggct tttgctgctg taattggtcc
     1441 gctggtggaa gtgccggtaa tgattgccct tgtcaacgtg gcattttggt tcaggaaaaa
     1501 gtggtttgcg tgaaaaaaaa gaggttgcaa acagattagc agcctctttt ttttgtatta
     1561 tcggaaatga aaagttacga aaactgagtt tattcaatgg ttgtaactca tccatcattg
     1621 ctgaatcatt ccgcgtctgt tcaggttgtt caacgggttc atgtttccac cggagttgac
     1681 cggccccatt gagttgttgg tattagttct gttagttgta cctgaaggtc tgtttgtttt
     1741 tgaagcagca tcagtagaat ttccagacga ttcgccgggg aagctcggac gtttcgtgct
     1801 ctcttgtctt tttggcggtt ccgttcgtac atattttgta aaaacatcat ccactttcag
     1861 ggggcgttct gtttcgtacc agaagaagtt acgcagataa agatcatttt cttccatctg
     1921 gctaatcgga tacatggcac cactggaggc tgtagtaagc acaattcgtt gaactttttt
     1981 atctttgaaa tacatggtga cgtaactgct caatgtttta tttatcccga tgtattcacc
     2041 tgttttatca tctttcggaa aataaatggt ttcggcattc ccgtttacat acactttgct
     2101 tagctgaccg ctatccaggt aagcggttat ttctttccct gataattgat tgaaataatt
     2161 cagactgtct ttttgaatgg caatgcccga ttgttcgatg tgtactttct ctaccttctg
     2221 attttttgtg taagcggtca ttttgttccc catcagctga ttattgtccg accataatac
     2281 cggcaaaacc cgaaggtgca gaatggaatc acgcgaagtg taaagcagcg aatcgcacat
     2341 accctgaata tcgcttcgga ggaatcgtac gtttttgaat gcttttacca tgttaaaaga
     2401 aacagaatcc gttgctacag agtctctcat tgagataagc gtatcggcgc tcatgaagag
     2461 tgaatcgcgg gttgaccagt cgatgagcag ggccgagtcg gtggcaagtc ctacacgcga
     2521 tatttcatcg taggatacaa agttgcctgt tagtgtcgat ttttgtttag gatcggtaag
     2581 ctccacatct gaaaatgctt caccgtattt tttcttttta tcgtaaaaaa tggtatcgcc
     2641 aatcagcgtc tttccgtcat tttgctccac caacgatctg ttgagcagca tcatgcggtc
     2701 atttttggtg tcgtaccagc ctcgctgact gtaaatgtcg gtttc
//
LOCUS       DAORKP010000214         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_11979_length_2740_cov_10.074860, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000214 DAORKP010000000
VERSION     DAORKP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11979_length_2740_cov_10.074860"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLN75_09085"
     CDS             <1..618
                     /locus_tag="PLN75_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRG04309.1"
                     /translation="GGCLTEKHLDTPIDYDNLLQVGSMMGSGGMIVMDEDDCMVSVAK
                     FYLDFIVEESCGKCAPCRIGNKRLNEILSKICDGNGTQEDLERLKNLSNVIKDTSLCG
                     LGQTSPNPVLSTLANFQEEYYEHVHDKKCRAGHCKNLLYYVIDPEACIGCMLCVRNCP
                     VNAITGERKQSHFIHQEICIKCGACMEKCKFDAISLTSEYAMAEH"
     gene            753..2525
                     /locus_tag="PLN75_09090"
     CDS             753..2525
                     /locus_tag="PLN75_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRG04310.1"
                     /translation="MEKIELTIDNKKIEVDKGTTVLKAARQAGIDIPTLCHMELHSMN
                     IENKPGGCRICVVEVEGRRNLAPSCITEVTPGMVIHTNNIRVLNARKTVLKLIISDHP
                     FDCLVCAKSGDCELQSLAIQCGIREIPYQGEKSTYRKDLSPSIIRDVDKCVMCRRCEM
                     MCNEVQTVGALSAVNRGFMSVVAPAFEMDLEKSPCTYCGQCVSVCPTGALTEVDHTSQ
                     VLRALSDPTKTVVVQTAPSVRAALGEEFGLEPGTLVTGKMAAALRQLGFKYVFDTDFA
                     ADLTIIEEGTELLGRLKSHLNGDKNVKLPILTSCCPGWVNFFEHNFPDLLDVPSTAKS
                     PQQMFGAIAKTYFADKIGVNRKDLIVVSIMPCLAKKYECQRDEFKVDGNPDVDFSMST
                     RELAHFIKQANIDFLNLPDEEFDAPLGESTGAGVIFGNTGGVIEAACRTAYELFTGKT
                     LDKVEFHELRGIEGIRSATIDFDGLPVNIGIAHGLGNARKLLNEVRAGRSKYHAIEIM
                     ACPGGCIGGGGQPFHHGHSEILKARAGAIYSEDERKTLRKSHENPYIVKLYKEFLGEP
                     GGHKAHSLLHTHYYDKSVEVTIEH"
BASE COUNT          790 a          590 c          667 g          693 t
ORIGIN      
        1 ggaggttgcc tcaccgaaaa gcacctggac accccgatcg actacgacaa cctgcttcaa
       61 gtcggatcca tgatgggttc gggaggtatg attgtgatgg acgaagacga ttgcatggtt
      121 tctgtggcca aattctatct cgatttcatc gtggaggaaa gttgtggaaa atgtgctccc
      181 tgccgtatcg gtaacaagcg tctgaacgaa attctttcca aaatctgcga cggcaacggt
      241 acgcaggaag acctcgaacg actgaaaaac ctcagtaatg taatcaaaga cacttctctc
      301 tgcggactcg gtcagacttc acccaatccg gtgctttcta cgctggccaa tttccaggaa
      361 gagtactacg aacacgtgca tgataagaaa tgtcgtgccg gacactgtaa aaatctgctg
      421 tattatgtta ttgatcctga ggcttgtatc ggttgtatgc tttgcgttcg taactgtccg
      481 gtgaatgcta ttacaggtga aagaaagcag tcgcacttta tccaccagga aatctgtatt
      541 aaatgtggtg cttgtatgga aaaatgtaaa tttgacgcca tcagtcttac ttctgaatat
      601 gctatggcag agcattagac tacagatgtt ttaattttaa cagaaacaga tttcaaaaaa
      661 ataaagatac actaaaggaa gattaacgaa agcataacag aaacagacat ctgatgtctg
      721 aaatctgtta tctgaaatct aaaaaaagaa acatggaaaa aatagaattg accatcgata
      781 ataagaaaat tgaagttgat aaaggtacga ccgtgctgaa ggcagctcgt caggctggga
      841 ttgacattcc tacgctttgc catatggaac ttcacagcat gaatattgag aacaaacccg
      901 gtggatgccg tatttgtgtg gtggaagtag aaggccgccg gaacctggca ccctcctgta
      961 ttacggaagt tacgcccgga atggttattc acaccaataa catccgcgtg ctgaatgccc
     1021 gtaaaacggt actgaaactg atcatttctg accacccttt cgactgtctg gtgtgtgcca
     1081 aatcaggcga ttgtgaactg caaagccttg ctattcagtg tggtatacgt gaaattccct
     1141 atcagggaga aaaatcaacc tatcggaagg acttgtcgcc gtccatcatc cgcgatgtgg
     1201 ataaatgcgt catgtgccga cgttgtgaga tgatgtgtaa cgaggtgcag accgtaggcg
     1261 cactttcggc tgtaaaccgg ggatttatgt ccgttgttgc tccggcattt gaaatggacc
     1321 ttgaaaaatc gccttgtacc tattgcggac agtgcgtttc cgtatgtccc accggcgctt
     1381 tgaccgaagt ggaccatacc agccaggtgc ttcgtgcgtt gagtgatcca accaaaaccg
     1441 tggtggtgca gaccgcacct tccgttcgtg ctgctttggg cgaagagttc ggactggagc
     1501 cgggaacttt ggttacagga aaaatggctg ctgcacttcg ccaacttggt ttcaaatatg
     1561 tgtttgatac cgatttcgca gccgacctta ccattatcga agaaggaaca gagttgctgg
     1621 gacgtttgaa aagccatctg aatggtgata aaaatgtgaa actgccgatt ttgacctcct
     1681 gctgtccggg ttgggtgaat ttcttcgagc ataatttccc tgacttgctg gatgttcctt
     1741 caacagccaa atcgccgcag cagatgtttg gcgccattgc caaaacctat tttgcagata
     1801 aaatcggcgt gaaccgcaaa gatttaatcg tggtttccat catgccatgt ctggcgaaaa
     1861 aatacgagtg tcaacgcgat gaattcaagg tggacggtaa tccggatgtg gacttctcca
     1921 tgtccacccg tgaattggcg cactttatca agcaagccaa catcgacttc ctgaacctgc
     1981 ccgatgaaga atttgatgct ccgctcggtg agtccaccgg agccggagtt attttcggga
     2041 atacgggtgg agtaatcgaa gcggcctgcc gcaccgcgta tgaattattt acaggaaaaa
     2101 cgctggataa agttgagttt catgaacttc gcggtatcga aggcattcgt tcggcaacga
     2161 ttgattttga cggactgccg gtgaatattg gtattgcaca cggattgggt aatgcccgaa
     2221 aactgctgaa tgaagttcgt gcaggacgat ccaagtatca cgccattgag attatggcat
     2281 gtccgggcgg ttgcattggt ggtggcggac agccgttcca ccacggtcac agcgaaattc
     2341 tgaaagcaag agcgggagcc atttattccg aagacgaacg gaaaactctc cgtaaatcgc
     2401 atgagaatcc gtacatcgtt aagctttaca aagaattttt gggagaaccc ggcggacata
     2461 aagcacattc gcttctgcac actcattatt acgataagag cgtggaagtt acgattgagc
     2521 attaacccct agcccctaaa tgggaaaggc aaagccgatt tagtttttat aaatattcaa
     2581 tgaaaaaaca ggaatttaat ccttttaaat gttgaactac gccctaaact cccccctcag
     2641 gggaatcggg agcaaaaaaa taagattatg acagtgcaaa caatgcccga aaataaatta
     2701 gaagtcaaga taaaattatc tgacgaaaag attgagaaaa
//
LOCUS       DAORKP010000215         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12129_length_2723_cov_7.654798, whole genome shotgun sequence.
ACCESSION   DAORKP010000215 DAORKP010000000
VERSION     DAORKP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12129_length_2723_cov_7.654798"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="fucI"
                     /locus_tag="PLN75_09095"
     CDS             <1..444
                     /gene="fucI"
                     /locus_tag="PLN75_09095"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HRG04311.1"
                     /translation="GGYSSNFLSKGEMPVTMTRLNLVKGLGPVLQIAEGWTVEIQEEV
                     HKTLNERTDKTWPTTWYAPRLTGSGAFKDVYSVMNNWGANHGAISYGHIGADLITLAS
                     MLRIPVCMHNVEEEKIFRPAVWNAFGMNVEGADYRACQNFGPIYK"
     gene            450..1784
                     /gene="fucP"
                     /locus_tag="PLN75_09100"
     CDS             450..1784
                     /gene="fucP"
                     /locus_tag="PLN75_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HRG04312.1"
                     /translation="MTDSKSIFVTSDGKNYRLPFILITCLFLLWGFAHSLLDVLNKHF
                     QDALDLTKAQSGLVQASAYGAYFLMALPAGFVARKYGYKKGVLFGLILFAIGCFWFVP
                     AVSIDKFWAFLFGLLIIFGGLTFLETVANPYTTVLGDPKYAASRINLAQTFNALGWIL
                     GPLIGSLLIFKSESTDNPFLSFGKTIRTLFTGVNESVVPVLPTADKAVDNTALIIPYV
                     GLGIVVLLVIVLFFLSMLPEVTPDSSGSDSNVEEGQQSLIKSKHFVYGVIAQFLYVAA
                     QTGIGSFFVNYVVEAESLGISEMQAGILLGLGGMGLFAVGRFSGSSLMKIIKPGRLLG
                     SFAAVNTLLMLVVATRHDNIGIIALILSYLFMSIMFPSIFALSIKGLGKKTKEASSVL
                     VMTIVGGAICPSLMGAIGASNMNVGFIVPMICFLYIFFFGFVGSKEKHAANQ"
     gene            complement(1818..2720)
                     /locus_tag="PLN75_09105"
     CDS             complement(1818..2720)
                     /locus_tag="PLN75_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG04313.1"
                     /translation="MGSILPARNKYVYLFSYFKGNGDGLHLATSFDGLKWEALGREDT
                     VLLKPEVGKDKLMRDPSIVQDDNGVFHMVWTSGWWDNGIGYASSKDLKHWSEQKYIPV
                     MEKFAGVKNTWAPELFYDRKDKLFYIFWASTVPGAFPEIPTSASEKGLNHKLYYVTTK
                     DFNTFSETRLFYNPGFSVIDGTMMERNNRYYMFVKNENSNPAEKNIRVVSNNKPFKFP
                     NKVSAPITGNYWAEGPAPLQVGDYVYVYFDKYRDKKYGAVRSSDMINWEDVSDSVTFP
                     KGIRHGTAFIVTESVFNQLIKQKN"
BASE COUNT          721 a          572 c          617 g          813 t
ORIGIN      
        1 ggcggatatt catccaattt tctttcgaaa ggtgaaatgc ctgtaaccat gactcgcctg
       61 aatctggtaa aagggctggg acctgtattg caaatagcag aaggatggac cgtagaaata
      121 caggaagaag tgcacaaaac gctcaacgaa cggactgaca agacctggcc aacaacctgg
      181 tacgcacccc ggctaaccgg ctcgggtgca ttcaaagatg tttattcggt gatgaacaac
      241 tggggagcca accacggagc aatcagctac ggccacatcg gggctgatct gattaccctg
      301 gcttcgatgc tgagaattcc ggtttgtatg cacaatgtag aagaagaaaa aatcttccgt
      361 cccgcagtct ggaatgcctt tggtatgaat gtggaaggag ccgattacag agcttgtcag
      421 aattttggac ccatttataa ataaacccaa tgactgattc taaatccatt ttcgtaactt
      481 cagacggtaa aaattatcgg ctgcctttta ttctcatcac ttgtttattt ttactgtggg
      541 gtttcgccca ttctctgctg gatgtgctga acaagcactt tcaggatgcg ctggatctca
      601 ccaaagcaca atccggatta gttcaggcgt cggcctacgg tgcatacttc ctgatggcgt
      661 taccggcagg atttgttgcc cggaaatatg gctacaagaa aggcgtattg tttgggttga
      721 ttctttttgc tataggctgt ttttggtttg ttccggctgt aagtatcgac aaattctggg
      781 cattcctgtt tggtttactc ataatattcg gtggacttac attccttgaa accgttgcaa
      841 atccttacac tacagttctg ggagatccga aatatgctgc cagcagaata aatctggctc
      901 agaccttcaa tgctttaggc tggatactcg gtccgcttat cggaagttta ttgattttca
      961 aaagtgaaag cacagacaat ccgtttttgt cttttggaaa aaccatcagg actttattca
     1021 caggagtcaa cgagtcggtc gtaccagttc tccccacagc ggataaggca gtggataata
     1081 cggcattaat tattccttat gtaggtttgg gaattgttgt tttactggta atagtccttt
     1141 ttttcctgtc aatgttgccc gaagttactc ccgattcatc cggttcagat tcgaacgtgg
     1201 aagaaggaca acaatcgctt ataaagtcga aacatttcgt gtacggagta atagctcagt
     1261 ttctttacgt ggcagcacag actggaatag gtagcttttt tgtaaactat gtggtagaag
     1321 cagaatcgtt gggaataagc gaaatgcaag ccggcatttt acttggatta ggaggaatgg
     1381 gattatttgc tgttggacgt ttttcgggca gttcgctgat gaaaatcata aaaccgggac
     1441 gattacttgg cagttttgct gccgtaaaca cactgctcat gctcgtagtt gcaacgaggc
     1501 atgacaatat tggtatcatt gcattgattt tatcctattt atttatgtcc atcatgttcc
     1561 cctccatctt tgccttaagt attaaaggat tgggtaaaaa aacgaaagaa gcatcgtcag
     1621 tgctggttat gacaatagtt ggaggtgcaa tttgcccctc gctgatgggt gcaataggag
     1681 cttcaaacat gaatgttgga tttatcgttc caatgatttg ttttctctat attttcttct
     1741 ttggttttgt tggaagcaaa gaaaaacatg cagcaaacca ataactggaa ttacataaat
     1801 caacgaaacg aaaatagtta gtttttctgc ttaatgagct gattgaaaac actttcggta
     1861 actataaacg cagttccgtg acgtatgcct ttggggaatg ttacagaatc ggatacatcc
     1921 tcccagttaa tcatgtccga agaacggaca gcgccatact ttttatcgcg gtacttgtcg
     1981 aagtacacgt acacgtaatc tcccacctgc agcggtgcag gaccttcggc ccaataatta
     2041 ccggtgatgg gagctgaaac cttgttggga aatttaaatg gtttgttgtt tgaaaccacc
     2101 cgtatatttt tctcggccgg gttcgaattc tcgtttttta caaacatata ataccggttg
     2161 ttacgctcca tcattgttcc gtcaataaca ctgaaaccgg gattgtaaaa caaccgtgtt
     2221 tcagagaatg tattgaaatc tttggtggta acgtagtaaa gcttatggtt cagtcctttt
     2281 tcgctggccg aagtgggtat ctcggggaac gcgccgggaa cggttgatgc ccagaagatg
     2341 tagaagagtt tatctttcct gtcgtaaaac aattcgggag cccaggtgtt ttttactccg
     2401 gcgaattttt ccatcacagg aatgtatttt tgttctgacc agtgtttcag atccttcgag
     2461 gaagcatacc cgatgccgtt gtcccaccat cccgatgtcc acaccatgtg aaatacaccg
     2521 ttgtcatcct gaacaatgct cgggtcgcgc atcagtttgt cttttcccac ttcaggtttc
     2581 agtaaaacag tatcctccct gcccaatgct tcccatttca gtccatcaaa actagttgcc
     2641 agatgcagtc cgtcgccgtt gcctttgaaa taagagaaca gataaacgta tttatttcgc
     2701 gcaggtaata ttgatccgat gcc
//
LOCUS       DAORKP010000216         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12365_length_2696_cov_7.490723, whole genome shotgun sequence.
ACCESSION   DAORKP010000216 DAORKP010000000
VERSION     DAORKP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12365_length_2696_cov_7.490723"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1110
                     /gene="surE"
                     /locus_tag="PLN75_09110"
     CDS             319..1110
                     /gene="surE"
                     /locus_tag="PLN75_09110"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRG04314.1"
                     /translation="MRPYILVTNDDGINASGILVLTRLMMQLGDVIVVAPDGPRSGQS
                     SAITVNVPIRFKEIEKKKGLTRYSCTGTPTDCVKLAFDELAERKPDLVVAGINHGSNA
                     AINVIYSGTMGAAFEGCEHGVPSIGFSLCDHSPDADFTEFEPHILRIASETLKNGLPQ
                     SVCLNVNAPVGKIGGIRVARQCHGQWMKEFAMKTDPNGKAYFWLTGYYQNFEPEAQDT
                     DEWALANGFISIVPSTIDLTAFQCIQTVRNWELEQTVPVSYPLSD"
     gene            1141..1425
                     /locus_tag="PLN75_09115"
     CDS             1141..1425
                     /locus_tag="PLN75_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04315.1"
                     /translation="MQRFNRFIFGFIPGILLPVLFLWIYLSRFYPADLSFFEIIKQLF
                     PGVMLGKLLLLSIMPNLIGVFIFYKQDNFKLGIGMMTGALPYLVTAMFMM"
     gene            1509..>2696
                     /gene="lpxB"
                     /locus_tag="PLN75_09120"
     CDS             1509..>2696
                     /gene="lpxB"
                     /locus_tag="PLN75_09120"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRG04316.1"
                     /translation="MGASSEGGGASSGKSSGLRYFLIAGEASGDLHASNLMCELKKMD
                     PDAEFCFLGGDLMTAQGGTMVKHYREMAFMGFVAVLKNARKVLNNLNECKQAIGRFRP
                     DVLILVDYPSFNLRIAKFAKETLNIPVYYYISPKIWAWKEFRIKAIKKYVDRMFTILP
                     FETEFYKKHDYEVTYVGNPSVDSIASRPNQKQTFAEFCRLNKLSELPVIALLPGSRRQ
                     EIAGCLPMMLEAVKKHSNHQVVVSGAPGIESDFYRTFITKTDINIVFNQTYELLTHAR
                     AAVVNSGTATLETALIGTPQVVVYKVAFPALAMPLKKIFIKVNFVSLVNLIARKEVVK
                     ELIGDLFTVENIRKELNRLLYAEKYRNGMVNNYREIQENLGPPGAARKAAEEMQKSLK
                     AEKE"
BASE COUNT          781 a          590 c          619 g          706 t
ORIGIN      
        1 ggagagttcg gagtttggag ttttgagttt tgagttttga gaaaaaaaaa tatataaaaa
       61 aatacttagt acgtgacaaa aattaaagaa atagctttac aattatgatt atcaacaata
      121 taagtattga aataattaaa agaataaaat tacgaatttc actaattgca cgaataaatt
      181 cgtgttattc gtctcattcg ttgttaaaag ttcaactcaa tgtgtatttt taagattcta
      241 ttattttttg tcatgtatta aaaaaaaata tacaatcaat cagctgactg ctgaaagcta
      301 aaaaactaac gtaacaacat gcgtccttat atattagtta caaacgatga cggcatcaat
      361 gcttccggaa tactggttct gacgcgtctg atgatgcagc ttggcgacgt aatagtggtt
      421 gcacccgacg gaccccgttc cggtcaatcc agtgccatta ccgttaacgt tcctattcgt
      481 ttcaaagaaa tagagaaaaa aaagggttta acgcggtatt catgcacagg tacacctacc
      541 gactgtgtga agttggcgtt cgatgaactt gccgaacgaa aacccgacct ggtggtggca
      601 ggcatcaatc acggttccaa tgctgccatc aacgttattt attcgggcac catgggagca
      661 gccttcgagg gatgcgagca cggagtgccc tccatcggat tctcgctctg cgatcattct
      721 cccgatgccg attttaccga attcgagccg catatcctca gaatagcctc cgaaacgctg
      781 aaaaacggat taccgcagag tgtttgcctc aacgtgaatg caccggtcgg aaaaatcggg
      841 ggaattcggg tagcacgtca atgtcacggc cagtggatga aagaatttgc catgaaaaca
      901 gacccgaatg gcaaagctta cttttggctc accggatatt accagaattt cgagcccgaa
      961 gcgcaggaca ccgatgaatg ggcgctcgca aacggattca tatccatcgt accctctacc
     1021 atcgacctta ctgcatttca gtgcattcag acagtgagaa actgggagct ggagcagact
     1081 gttcctgttt catacccgct gtccgattga tttgattttc aataacttat taacagaaaa
     1141 atgcaacgct tcaatcggtt tatcttcgga tttattcccg gtattttatt gcccgtgctg
     1201 tttctgtgga tttatctgag ccggttttat cctgccgatt taagcttttt cgaaatcatc
     1261 aaacaacttt ttccaggggt gatgttgggt aaacttttgc tgctcagcat tatgccgaat
     1321 ctcatcgggg tttttatttt ttacaaacag gataatttca aattgggaat tggaatgatg
     1381 acaggcgcac taccctacct ggtgactgcg atgtttatga tgtaaggccc ctgttgagtt
     1441 atgagtaatg agttatgagt aaaaaacaac ataccggcaa aaatgcacaa atcccccctt
     1501 cggggggtgt gggggcatct tcggaaggtg gaggggcttc ttccgggaaa tccagcgggc
     1561 ttcgctactt cctcattgcg ggcgaagctt ccggagattt gcacgcttcc aacctcatgt
     1621 gcgaactcaa aaaaatggat ccggatgcgg aattttgctt tttaggcggt gatttgatga
     1681 ctgctcaagg cggaacgatg gtgaaacatt atcgcgaaat ggcatttatg ggttttgtag
     1741 ctgtactgaa aaatgcccgg aaagtactga acaatttgaa tgaatgtaag caagctatcg
     1801 gacgttttcg ccctgatgta cttattctgg tggactaccc ttctttcaac ctccggatag
     1861 caaaatttgc gaaggaaacg ctgaatatcc cggtttacta ctacatatcg cctaaaatct
     1921 gggcctggaa agagtttcgc atcaaggcga taaaaaaata cgtggaccgg atgttcacga
     1981 ttctcccttt cgaaaccgaa ttttacaaaa aacacgatta cgaagtgacc tacgtgggca
     2041 atccgagtgt ggatagcata gccagccgcc ccaaccaaaa acagactttt gctgaatttt
     2101 gccggctgaa caagctctct gaactgcctg taattgcact tttgcccggc agccggaggc
     2161 aggaaattgc gggttgtcta cccatgatgc tcgaagcggt aaaaaaacac tcgaatcatc
     2221 aggtggtggt tagcggcgcg ccgggtatcg aatcggattt ttaccgtaca tttatcacaa
     2281 aaacagatat aaacatagtc ttcaatcaaa cctacgaact actcacccac gcccgcgccg
     2341 ccgtggtcaa ttccggtacg gccacgctcg aaacggcgct gataggaacc ccgcaagtgg
     2401 tggtatataa agtggctttt cctgcgctcg ccatgccgct gaaaaagata ttcatcaaag
     2461 tcaactttgt gtcgctggtg aatctgattg ccagaaaaga agtagtgaaa gagctgattg
     2521 gtgacctttt cacggtagaa aatattcgca aagagctgaa cagactcctt tacgccgaaa
     2581 aataccgcaa cggaatggtg aacaattacc gggagattca ggaaaacctc ggccctcccg
     2641 gagccgcccg caaggcagcc gaagagatgc agaaaagtct gaaagctgag aaagaa
//
LOCUS       DAORKP010000217         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12414_length_2690_cov_8.856546, whole genome shotgun sequence.
ACCESSION   DAORKP010000217 DAORKP010000000
VERSION     DAORKP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12414_length_2690_cov_8.856546"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLN75_09125"
     CDS             <1..470
                     /locus_tag="PLN75_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRG04317.1"
                     /translation="YSNFQLIALVFLRVVTGWYFLYEGLAKILSPGWTSYGYLMDSQG
                     VFSEFFKSLGQNQTLMPVVDALNVYGLILIGLLLIVGLFEKIGYIGALLLLVMYYLSH
                     PASLNVTYLLPPEGSYLWINKNIVMMFAILVLMAFPSARRIGIDRFIFRNKKA"
     gene            482..2143
                     /locus_tag="PLN75_09130"
     CDS             482..2143
                     /locus_tag="PLN75_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG04318.1"
                     /translation="MSTKENTGNENTKNTENSENSFSKGRREALKTLATVPVLGAMAY
                     GVYKKSKKERTNRLAADMFHFGEMPEPVIVADGEIIRIGVIGVGIRGIDLMQAIGFAT
                     PEHLEDLKKQAQNNSGDTRYKDFMEQDDLRIKITAVCDIFDTYAERAITAGANIHREG
                     LNGKYGEKPKRYLNYKELLAAPDVDAVIIAAPDHWHGTMAMEAIQAGKHVYVEKPMTW
                     TVPETYALREVVRNSKLTFQLGHQNRQIETNKRGKEIIGKGLLGPITLIETSTNRNDP
                     NGAWVYPIHADANPRTIDWKQFEGEPARVREYMDYMTSNNLAKYVGPDARDKFSLERF
                     FRWRCWWDYSTGLSGDLLTHEFDAINQMMNLGIPDSATSSGGVYFFKDGRTVPDVLQT
                     TFEYKEKNLALLYSATLANQFNRPRKFMGHDATMEIGNSITVTVDPQSEQYKDQIEKG
                     LIKPGKPFYSYTPGQKDLDAISSATQLYFAERGLLYSYVNGKRYNTTFLHLKEWIDCI
                     RTGKKTSCGIEEGFQEAITAHMGTRAYLEGRTMYWDGDKEEIVRG"
     gene            2279..>2690
                     /locus_tag="PLN75_09135"
     CDS             2279..>2690
                     /locus_tag="PLN75_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG04319.1"
                     /translation="MSTMSRRKFLKTGAIAAAGLTVVPSSVLGQKFGKISPSDKLNIA
                     GVGVGGRGFSVLKALESQNIVGLCDVDWKYSDKAFKHFPNAKKYFDYRKMYEEIGKSI
                     DAVVVATADHTHAIIAADAMTMGKHVYCEKPLTHS"
BASE COUNT          826 a          589 c          637 g          638 t
ORIGIN      
        1 cgtattcaaa ttttcagttg atcgcccttg tttttttgag agttgttacg ggctggtatt
       61 ttttgtatga aggtttggca aaaatactct cacccggttg gacctcttat ggttatctga
      121 tggattcaca gggcgttttt tccgaatttt tcaaatcgtt gggacagaat cagacattaa
      181 tgcccgtagt ggatgcattg aatgtttacg gattgattct catcggattg ctgctgattg
      241 taggattgtt tgaaaaaatc gggtacatcg gagcgctgct tctgctggtc atgtattacc
      301 tctcacatcc ggcctcgctg aatgttacct atctgttacc ccccgaaggt tcgtatttgt
      361 ggattaataa aaatatcgtg atgatgtttg ccatccttgt tctcatggct ttcccatcag
      421 cccggcgaat aggaatagac cggtttattt tcagaaacaa aaaagcgtaa tacatccgaa
      481 tatgtcaaca aaagaaaata caggaaacga aaatacaaaa aacaccgaaa attcagaaaa
      541 tagcttttcg aaaggcagac gcgaagcact caaaacactg gcaaccgtgc cggtacttgg
      601 cgcaatggct tacggagtgt ataaaaaatc caaaaaagag cgcaccaacc ggcttgctgc
      661 cgatatgttt catttcggcg aaatgcccga accggtcatc gtggcggatg gtgaaatcat
      721 cagaatagga gtgataggcg tcggaatcag aggcattgac cttatgcagg caatcggatt
      781 tgccacaccc gaacatcttg aagatttgaa aaaacaagcg cagaacaact ccggcgatac
      841 acgttataag gatttcatgg agcaggatga cttacggatt aagataactg ccgtgtgcga
      901 tattttcgat acctatgccg agcgagccat taccgccgga gccaacatac accgcgaggg
      961 actgaacgga aaatacggtg aaaaaccgaa acggtatttg aattacaaag aactgctcgc
     1021 cgctcccgat gtggatgcgg taataatagc cgctcccgac cactggcacg gaacaatggc
     1081 tatggaagcc attcaggccg gtaaacacgt atatgtggag aaacccatga catggactgt
     1141 acccgaaaca tatgcgctta gagaagtggt gagaaacagc aaactgacat ttcagctcgg
     1201 acatcagaac cgacagatag aaaccaacaa acggggtaag gaaatcatcg ggaaaggact
     1261 tctcggcccc attaccctca tagaaaccag caccaaccgc aacgacccga acggagcctg
     1321 ggtgtacccc attcatgccg atgcaaaccc acgaaccatc gactggaaac agtttgaagg
     1381 cgagccggca cgtgtgaggg aatacatgga ttacatgact tccaacaacc tggcaaagta
     1441 cgtgggaccc gatgccagag acaaattcag cctggagcgc ttcttccgct ggcgatgctg
     1501 gtgggattac agcacagggc tgagcggcga cctgctcaca cacgaatttg atgccatcaa
     1561 tcagatgatg aacctcggca ttcccgattc tgccacttca tcgggtggag tctatttttt
     1621 caaagacggc agaacggtac ccgatgtgtt acagactact tttgaataca aagaaaaaaa
     1681 tctggcgctg ctttattcgg ctacgctggc caatcagttc aatcgcccgc gcaaattcat
     1741 gggacacgat gccaccatgg aaataggcaa ttcaatcacc gtaactgttg atccacagtc
     1801 ggaacaatac aaagatcaga ttgagaaggg attgataaaa cccggtaaac cattctacag
     1861 ttatacaccg ggtcagaaag acctggatgc catatcatca gccacacagc tttactttgc
     1921 cgaaagaggg ttgctttatt cgtatgtaaa cggaaaaaga tacaatacca ccttcctgca
     1981 cctgaaagaa tggattgatt gcatccggac gggtaaaaaa acatcctgtg gaattgaaga
     2041 aggattccag gaagcaatca cagcccacat gggtacacgc gcgtacctcg aaggcagaac
     2101 catgtactgg gatggtgata aggaagaaat tgtaagaggg tgataagaag atggaagttc
     2161 tgaattctga attctgagtt ctgagtattg agttacgttt ttatctgttt attcgtctgt
     2221 gttctatcag cgcagcggtc taataaaaaa cactattaat cttaaaaaat aaatgcctat
     2281 gtcaacaatg tcaagaagaa aattcctgaa aaccggtgcc attgcagcag ccggtctgac
     2341 ggtagtaccc tcgtcggtac ttggacaaaa attcggtaaa atatctccaa gcgacaagct
     2401 gaatattgca ggagtaggag tgggaggacg cggttttagc gttttgaaag ctcttgagag
     2461 tcaaaacatt gtagggcttt gtgatgtgga ctggaagtac agcgacaaag cctttaagca
     2521 tttcccgaat gccaaaaagt acttcgatta ccgtaaaatg tacgaggaaa tcggtaaaag
     2581 cattgatgca gtggtagttg ctaccgctga tcatactcac gcaataatag cagccgatgc
     2641 catgacaatg ggaaaacacg tttattgcga aaaaccattg actcacagcg
//
LOCUS       DAORKP010000218         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12423_length_2689_cov_8.665528, whole genome shotgun sequence.
ACCESSION   DAORKP010000218 DAORKP010000000
VERSION     DAORKP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12423_length_2689_cov_8.665528"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1206)
                     /locus_tag="PLN75_09140"
     CDS             complement(160..1206)
                     /locus_tag="PLN75_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HRG04320.1"
                     /translation="MENAIRNYIFRQFKKSETKKHELNYLFWECTTRCNLNCRHCGSD
                     CSKDSLHKDMPLEDFLKALETIENPAENFTVVLTGGEPLLRKDIEQCGREIRKRGMRW
                     SMVTNGFSYDEEKHISLLNAGMGALTISLDGLESSHNWLRNNQKSFERVDKAINFAAS
                     APRLNFDVVTCVNQRNFTELEEIKQYLIRKKVKAWRLFTIVPIGRAKENPELFLTDNQ
                     FNALMEFICQSRKTDEIDVKFSCEGYVGKYEWKVRDAGFFCRAGINIGSVLIDGSISA
                     CPNIDRTFAQGNIYTDNFREVWNNRFEPFRNREWAHTGKCTDCDMWRYCEGNGMHLHD
                     DEGKLLVCHYQRIE"
     gene            complement(1207..1575)
                     /locus_tag="PLN75_09145"
     CDS             complement(1207..1575)
                     /locus_tag="PLN75_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04321.1"
                     /translation="MKSNWKKNLFKGLCLTSVAFVFQACYGTPQDLGYDLLIEGKVIS
                     KNTGLPVKGIKISVADGPQYVNSDNEGNFGFYTFAQDSIKLTFEDIDSTENGQFIKKD
                     TVLKVTDEKIMLNIALDEKE"
     gene            complement(1618..2688)
                     /locus_tag="PLN75_09150"
     CDS             complement(1618..2688)
                     /locus_tag="PLN75_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HRG04322.1"
                     /translation="MNKTPSLRKKIGLEIFRKMRHNNAHLHELTTLFWECTLRCNLSC
                     LHCGSDCHVQSDKKDMPIDDFMRVIDEITPHVNPHKTMIIFTGGEALLRKDLEICGKQ
                     LYYRGFPWGLVSNGMALTQQRLNSLLISGLHSITISFDGFEEAHNWLRNNPKSYANAL
                     NAIKLLTKVKDIAWDVVTCVNKKNISTLPEFKEFLIETGVKEWRIFTIFPVGRAAEHA
                     ELQLSNEEFTSVLDFIRDTRKENRIHLSYGCEGFLGNYEQEVRDHFYTCHAGINVASV
                     LADGSISACPSIRSNFNQGNIYTDNFWEVWNNRFEPFRNREWAHTGKCTDCDMWRYCE
                     GNGMHLHDDDGKLLVCHYGRIE"
BASE COUNT          756 a          592 c          452 g          889 t
ORIGIN      
        1 cgtccgttta ctttttcgga aaaagtcagg atgacctcat gtataatttc tgcttacagt
       61 aactttttat ccatttgaat tagtcaattt tcacctgact tagcacgcgg taaaattgcg
      121 tagcagcgga ggtttaaaaa aacttttgca atcctatttt cattcaatcc gctgataatg
      181 acaaaccagt aatttgcctt catcgtcgtg taaatgcata ccgttacctt cgcagtagcg
      241 ccacatatcg cagtcggtgc atttacctgt gtgcgcccat tcacggttgc ggaaaggttc
      301 gaaacggttg ttccacactt cccggaaatt atcagtataa atatttccct gagcaaaagt
      361 tctgtcgata ttcggacaag ctgaaataga tccatcgatt aaaacagaac cgatattgat
      421 gcctgcacga caaaagaaac ctgcatcgcg tactttccat tcgtatttcc ctacataacc
      481 ttcgcaactg aattttacat caatttcatc agtttttctc gattgacaga taaattccat
      541 taatgcatta aattgattat cagtcagaaa taattccggg ttttcttttg cccgtcctat
      601 tggaacaatg gtaaacaagc gccatgcttt cacttttttc cgaatcaaat attgctttat
      661 ttcttccaat tcggtgaaat ttctttggtt tacacaggtt actacatcaa aattcaatcg
      721 tggtgcgctc gctgcaaaat tgatagcttt atcaactctt tcaaaacttt tctggttgtt
      781 tcttagccag ttgtgcgaac tttccaaacc atccaggctt attgtgagtg ctcccatacc
      841 tgcatttaac aacgaaatat gtttttcttc atcgtacgaa aatccatttg ttaccatgct
      901 ccaacgcatg cctcttttgc ggatttcccg tccgcactgt tcaatgtctt tccgtaaaag
      961 cggctcaccg cctgtgagaa caacggtaaa attttccgca ggattttcaa tggtttcaag
     1021 cgctttcaaa aaatcttcca atggcatatc tttatgcaaa ctatctttcg agcaatcgct
     1081 tccacaatgc ctgcaattca gattacacct tgttgtacat tcccaaaaca gataattcaa
     1141 ctcatgtttt ttggtttccg attttttgaa ttgcctgaaa atataatttc ttattgcatt
     1201 ttccatttat tctttttcgt caagtgcaat attcagcatt attttttcgt cggttacttt
     1261 cagtacagta tcttttttga taaattgacc gttttcagtg ctgtcaatgt cttcgaacgt
     1321 cagtttaata ctgtcttgcg caaacgtgta aaacccaaaa ttgccttcgt tatcagaatt
     1381 aacatattgc ggaccgtctg caacagaaat tttaatgccc ttgacaggca aacctgtatt
     1441 cttcgaaatt acttttcctt cgattaataa atcgtaaccc aaatcctgtg gtgtgccata
     1501 acatgcctga aatacaaagg ctaccgaggt caaacacaat cctttaaaca aatttttttt
     1561 ccagttactt ttcatgattt tacttttatt tttttttaaa acaaaattgt aacttattta
     1621 ttctatcctt ccgtaatgac aaaccagtaa tttaccatca tcatcgtgca aatgcatacc
     1681 gttaccttcg cagtagcgcc acatatcgca gtcggtacat tttccggtgt gcgcccattc
     1741 gcggttgcgg aaaggttcga aacggttgtt ccacacttcc caaaaattgt cagtatagat
     1801 atttccctga ttgaaattgg atcgaatact cgggcaggct gaaatggatc catccgcaag
     1861 cactgaagcc acatttattc cggcatgaca ggtataaaaa tggtcgcgga cttcctgttc
     1921 gtaatttccc aaaaatcctt cgcaaccata acttaaatga atgcggtttt cttttcgggt
     1981 atcgcgaata aaatcaagta cggaagtaaa ttcttcgttc gagagctgta attctgcatg
     2041 ttcagcagct ctgccaaccg gaaaaatggt gaaaatccgc cattccttta cgcctgtttc
     2101 aatcagaaat tccttgaatt caggtaaggt gctgatattt ttcttattca cacaagtaac
     2161 cacatcccat gcaatgtctt tcaccttagt tagcaattta atggcattga gcgcattagc
     2221 atagctttta ggattattgc gtagccagtt atgggcttcc tcaaatccat cgaaacttat
     2281 ggtgatagag tgtaagcctg aaatgagtaa cgaattaaga cgttgctgag taagcgccat
     2341 tccattcgaa accagtcccc atgggaaacc tctgtagtac aattgttttc cgcaaatttc
     2401 caaatcttta cgcagcaaag cttcaccacc ggtaaaaatt atcatggttt tgtgcggatt
     2461 tacatgtggt gtgatttcat caatcactct cataaaatca tcaatcggca tgtctttttt
     2521 gtcggactga acatgacaat cgcttccaca atgcagacac gataaattac aacgcagtgt
     2581 acattcccaa aacaaagttg tcagttcgtg taaatgagca ttgttatgtc tcattttacg
     2641 aaaaatttca agtccgattt ttttgcgaag cgatggagtt ttattcatt
//
LOCUS       DAORKP010000219         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12580_length_2673_cov_8.229947, whole genome shotgun sequence.
ACCESSION   DAORKP010000219 DAORKP010000000
VERSION     DAORKP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12580_length_2673_cov_8.229947"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLN75_09155"
     CDS             complement(<1..143)
                     /locus_tag="PLN75_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRG04323.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPIVITNSEGKRTTPSVVGFVE
                     GGER"
     gene            complement(386..868)
                     /locus_tag="PLN75_09160"
     CDS             complement(386..868)
                     /locus_tag="PLN75_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04324.1"
                     /translation="MKKIILTIICTLFGLFSTFSQENTLEITPKIKHHSIYLELGGNS
                     GAYSVNYDYSLSVSESAKLAVGTGLSALSLYSYNDGPAPVQNTQFFITPEANLLFGKN
                     SHHLETGVSLLLFQIPALRVGYRYQPAKGGFLFRAGFTPLLFRMEIFPWGGISFGYTF
                     "
     gene            complement(953..>1438)
                     /gene="rimM"
                     /locus_tag="PLN75_09165"
     CDS             complement(953..>1438)
                     /gene="rimM"
                     /locus_tag="PLN75_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445025.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRG04325.1"
                     /translation="QITKAHGLKGELSFTTNSTILEDVEVPFVILEPEGLLVPFYIES
                     VRMKTDTTGLLKLERVDTEEQASEYAGLTIYLPNMFLDEIEDAEIETEYFVGFEVTDE
                     EKGSIGRVKAVDQSTANALFVVETESGEVLIPVAEEYITDIDHDRKIIFLKLPEGLLD
                     L"
     assembly_gap    1439..1538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1539..1888)
                     /gene="greA"
                     /locus_tag="PLN75_09170"
     CDS             complement(<1539..1888)
                     /gene="greA"
                     /locus_tag="PLN75_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRG04326.1"
                     /translation="MATTYMTAEGYKKLVQDLNDMENVQRPAISKLIAEARDKGDLSE
                     NAEYDAAKEAQGMLEMKIAELKDIIGSARMIDESRISTDEVQILTRVKIKNTRNNQIM
                     TYQIVSENEANLKEG"
     gene            complement(2045..2560)
                     /locus_tag="PLN75_09175"
     CDS             complement(2045..2560)
                     /locus_tag="PLN75_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04327.1"
                     /translation="MEDIGDYIYFLLLAVFALSGLFGKKKKDTKTSEKKKPVFDQIPK
                     SWEELEEMLDKPQPKPQPATTARPVTIPRSEPARPSTPFGSAEVISSPEYETTGYETM
                     SYDTATDFSTLRAKKQIKESIFKTKTTFVDTLSESESATEFSPFTIDLANTEEAQKAF
                     IYSEIFQRKYK"
BASE COUNT          690 a          511 c          543 g          829 t
ORIGIN      
        1 cgttcgccac cttccacaaa acccacaaca gaaggagttg tacgttttcc ttcgctgttt
       61 gtgataacga taggttcgtt accttccatt acggaaacac acgagttggt ggttcccagg
      121 tctattccaa ttatttttcc cataatgatt taaaaaatgt attttgtatt ttatttaatt
      181 cgtgtaaaaa atgttacgct tcttttttta caaatgatat gccatgcctg aaaaattgat
      241 ttataaaaaa cttttggcag aaaatcggta aaaatgggcc ggattaaaac gggaaaactg
      301 acaaaaaggc agtttttcgg acaaaaaaat actcacagcg cgattcgggc agtgagtatc
      361 gggtgtcggg aggatgtttt tttatttaga aagtatatcc gaatgaaatg ccaccccagg
      421 gaaaaatttc cattctgaaa agtaaaggag taaatccggc gcgaaaaaga aagcctcctt
      481 tcgcaggctg ataccgatat ccgactctga gtgccggaat ctgaaagagt aataaactta
      541 caccggtttc aaggtggtgc gaatttttac caaacaacag gttggcttcg ggtgtaatga
      601 aaaactgagt attctggacg ggggcgggtc catcgttata tgaatacagc gagagagctg
      661 ataatccggt gcctacagcg agtttcgcac tttctgaaac agacagggaa tagtcgtaat
      721 tgactgaata ggctccggaa tttcctccca attcaaggta gatggaatgg tgtttgattt
      781 tcggagtaat ttccagggtg ttttcctgag aaaatgtgga aaaaaggccg aataatgtac
      841 agataatagt caggattatt ttcttcatga ttgatggttg atgatgtcgt ttattaattg
      901 atggatttat accccaaaac gctgcaagtg cagtagttaa ataaacttaa atttataaat
      961 cgagcaatcc ttcgggcaat ttcaggaaga ttatttttct gtcgtgatca atgtcggtga
     1021 tgtattcctc agctacaggt atcagcactt ctcccgattc ggtttccacc acaaagagag
     1081 cattagctgt gctctggtca actgccttta cccgtccgat acttcctttt tcttcgtcag
     1141 tcacttcaaa gcccacgaaa tattcagttt ctatttcagc gtcttctatt tcatccagaa
     1201 acatgttggg caggtaaatg gtaagtcctg cgtactcgct agcctgttct tcggtgtcca
     1261 cccgctccag cttgagcagc ccggtggtat cggttttcat gcgcacgctt tcgatgtaga
     1321 agggcaccag cagtccttcc ggttcgagga ttacaaacgg cacttccaca tcttcaagta
     1381 tggttgaatt cgttgtgaat gacagttcgc ctttcagccc gtgcgctttg gtgatttgnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ttctttgagg tttgcttcgt
     1561 tttccgaaac aatttgataa gtcattatct gattgtttct ggtgtttttg atttttacac
     1621 gcgttaaaat ctgtacctcg tcggtcgata tccggctctc atctatcatg cgtgccgaac
     1681 cgatgatgtc tttcagctcg gcaattttca tttcgagcat gccctgtgct tctttcgcag
     1741 cgtcgtattc ggcattttca gataaatctc ccttgtcgcg tgcttcggct atcagttttg
     1801 agattgccgg ccgctgtaca ttttccatat cattcagatc ctggactaat tttttatacc
     1861 cttcggcggt catgtatgtt gtggccataa tggtgatagt ttaagttagt gaaaaacaaa
     1921 gagaattcca acaaaataaa gttggaactc tccctttagg tttaattaca aatacaaaga
     1981 taaaatcttt tttttgaaaa acaaaattat tggataaaaa tgccttttcg ttgaaaaata
     2041 gattttactt atattttcgt tgaaaaattt cggagtagat aaatgccttt tgtgcttctt
     2101 ctgtgtttgc caaatcaatg gtaaaaggac tgaactctgt ggctgattcg ctttcggata
     2161 gtgtgtccac gaaagtagtt ttggtcttga aaatgctttc tttgatttgt tttttggctc
     2221 tcagcgtgct gaaatcagtt gccgtatcgt agctcatggt ctcatatccg gtagtttcgt
     2281 attcgggcga cgaaatgact tctgccgaac caaaaggtgt ggagggtctc gctggctctg
     2341 atcggggaat cgtgactggt cttgccgtag tggctggctg tggtttcggc tgaggcttgt
     2401 cgagcatttc ctccagttcc tcccacgatt ttggaatctg gtcaaagacc ggttttttct
     2461 tctctgaagt tttagtatcc tttttctttt tcccgaaaag gccgcttaaa gcaaaaactg
     2521 ctaaaagaag gaaataaata taatcaccaa tatcttccat agttctcaat tttcttgtgc
     2581 aatattacaa aaaaaatatg atatacggtt agttttataa aaaaaacaat gggtaacttc
     2641 acagccaccc attgctacat tttaacctaa acc
//
LOCUS       DAORKP010000220         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12684_length_2660_cov_9.059501, whole genome shotgun sequence.
ACCESSION   DAORKP010000220 DAORKP010000000
VERSION     DAORKP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_2660_cov_9.059501"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLN75_09180"
     CDS             <1..868
                     /locus_tag="PLN75_09180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRG04328.1"
                     /translation="KAKKPDKIIYCYWMAFMAPCFGTIARFAKSPTTKSIALVHNMIP
                     HEPTILDKLFPRYFVKAMDGFVAMTASVVEDINKFDNKNKPKLVSPHPIYDHYGAITP
                     RSEALDALDLDPAFRYVLFFGFIRKYKGLDLLMEAFADVRLRQFPVKLLVAGEFYEPK
                     EPCFEMIRKYALEDLVVLHTDFIPEKEVAFYFNAADLVAQPYRSATQSGVSQIAYHFE
                     KPMLVTNVGGLAETVPNGKVGYVVEPEVSKIADALVDFFENNRSTKFIENLKEEKKKY
                     DWKLMVETVNKI"
     gene            962..1543
                     /locus_tag="PLN75_09185"
     CDS             962..1543
                     /locus_tag="PLN75_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRG04329.1"
                     /translation="MEQLSLQSKIHTIRNVQVMLDYDLAALYEVSTKQLNLAVKRNSE
                     RFPDDFMFQLTLEEWNALRFQFETSNTRGGRRYAPYAFTEQGLAMLSGILNSPVAIQV
                     NIAIMRTFVAIRQSIMPLGSNSNMKQIENKIMVLEAVSEETLAALNDLSEDTRKEFDD
                     IYIALSEMADKQKQITKPRRKIGYIQDTEEEKL"
     gene            1540..>2660
                     /locus_tag="PLN75_09190"
     CDS             1540..>2660
                     /locus_tag="PLN75_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRG04330.1"
                     /translation="MTNDVVETPMMKQFFDIKRKYPDAVLLFRCGDFYETYSTDAVEC
                     AGILGITLTRRANGSAKTVEMAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLTKKI
                     VKRGVTELVTPGVSYSDTTLTQKENTFLASVYLNKNQAGVSFLDISTGEFLIAEGTVD
                     YVDKLLSSFSPKEVIYDRSKRSEFEANFGTKYFTFALEDWGFSPETAYERLLKHFQTK
                     NLKGFGVENLKNGIVAAGAILHYLDMTHHLQTEHITHLTRIEEERFVWLDRFTVRNLE
                     LYGTINEGGRTLVDVIDRTISPMGGRMLKRWVAFPLKDVKPIEERLSVVEYFFRDTEL
                     KHMLEQQIALIGDLERIISKVAVGRINPREVVQLKVALQA"
BASE COUNT          798 a          543 c          623 g          696 t
ORIGIN      
        1 taaagcaaag aaaccggata aaatcattta ttgctactgg atggctttta tggcaccctg
       61 tttcgggacg attgcccggt ttgcaaaatc tccaacaacc aaaagcattg cgctggttca
      121 caatatgata cctcacgaac caactattct ggataagttg tttccacgtt attttgtcaa
      181 agcgatggac ggatttgtgg ccatgacggc ttcggtggtg gaagacatca ataagttcga
      241 taacaagaat aaaccaaagc tggtttctcc gcaccccatt tacgaccatt acggagccat
      301 tacgccccgt tcggaagcgc tcgatgcgct ggatctggat cccgcttttc gctatgtttt
      361 atttttcggt tttatcagga aatacaaagg gctcgacctg cttatggaag cctttgccga
      421 tgtacggttg cgccagttcc cggtgaaact gctcgttgcc ggtgaatttt acgaacccaa
      481 ggaaccttgc ttcgaaatga tcagaaagta tgcgttggag gatttggtgg tgcttcacac
      541 cgattttatc cccgaaaaag aagtggcttt ttatttcaat gcggccgatc tggtggcgca
      601 accataccga tcagctactc agagcggcgt ttcgcagatt gcttaccact tcgaaaaacc
      661 catgctggtg accaacgtgg gcggtctggc agaaacagtt ccgaatggaa aagtaggtta
      721 cgtggtggaa cccgaagtga gcaaaattgc ggatgcgctg gtcgattttt ttgaaaataa
      781 tcgctccact aaatttatcg aaaatttgaa agaagaaaag aaaaagtacg actggaagtt
      841 gatggtggaa actgtaaata aaatttaata tatttcagtt gtcccaaaaa atcggacaac
      901 tgaaaatacc tcctgaccat cgttaaaatc agatattcaa cgaattaaaa gaatacatta
      961 aatggagcaa ctttcacttc aaagtaaaat tcacactatt agaaatgttc aggtaatgct
     1021 ggattacgac cttgcagcat tgtatgaagt tagtacgaag cagttaaatt tggcagtaaa
     1081 aagaaacagt gagcgtttcc ctgatgattt tatgtttcaa cttacactgg aagaatggaa
     1141 tgctttgagg tttcaatttg aaacctcaaa tacaagaggt ggacggcgat atgctcctta
     1201 tgcttttaca gagcagggat tagccatgct ttccggtatt ctgaatagtc cggtagccat
     1261 acaggtaaat attgccatca tgcgtacttt tgtggccatt cgtcaatcga ttatgccatt
     1321 agggtcaaat tccaatatga agcaaatcga aaataaaatc atggttctgg aagctgtaag
     1381 tgaagaaaca ttggcagcat taaatgattt gtcggaagat acgcgaaaag aattcgatga
     1441 catatatatt gctctgagcg aaatggctga taagcaaaaa caaataacca aaccaagaag
     1501 gaaaattggt tacatccaag atacagaaga ggagaaatta tgacaaacga cgtggttgaa
     1561 accccgatga tgaagcagtt tttcgatatt aagcgaaaat atcccgatgc ggtgctgctt
     1621 tttcgttgcg gtgattttta tgaaacatat tcgacggatg ctgtggaatg cgccggaatt
     1681 ttgggcatca cgcttacccg ccgtgccaac ggatcggcta aaacggtgga aatggccggt
     1741 tttccgcacc atgcactgga tacttatctg cccaaactcg tccgtgcagg caagcgtgtt
     1801 gctatttgcg accagctgga agacccgaaa ctcaccaaaa aaatagtgaa acgcggtgtc
     1861 accgaactgg ttacgcccgg ggtttcatac agcgatacta cgcttactca aaaggaaaac
     1921 accttcctgg caagtgttta tctgaataaa aatcaggcag gagtatcttt tctcgatata
     1981 tcaacaggtg aatttttgat tgccgaaggt actgtggatt atgtggataa attgttgagc
     2041 agtttctcgc ccaaagaagt tatttacgac cgttcaaaac gcagtgagtt tgaagctaac
     2101 tttggaacaa aatatttcac tttcgcactc gaagactggg gattttcgcc cgaaacagcc
     2161 tacgaacggt tgctgaaaca tttccagacc aaaaatctga aaggtttcgg tgtggaaaat
     2221 ctgaaaaacg gtattgtagc agccggtgct atcctgcatt acctggacat gacacaccac
     2281 ctgcaaaccg aacatattac ccacttgacg cgtattgaag aagaacgttt cgtgtggctc
     2341 gaccggttta cggtacgcaa cctggaattg tacggcacca tcaacgaggg cggacgtaca
     2401 ctggtggatg tgattgaccg taccatcagt cctatgggag gccgaatgct gaaacgctgg
     2461 gtggctttcc cgctcaaaga tgtgaaaccc atagaagaac gtcttagcgt agtggaatat
     2521 tttttcaggg atacggagtt aaaacacatg ctggaacagc agattgcatt aatcggcgat
     2581 ttggagcgga taatctcaaa agtagctgtc ggacgtatta atccgcgcga agtggtgcag
     2641 ctcaaagtgg ctttgcaggc
//
LOCUS       DAORKP010000221         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12752_length_2651_cov_7.319337, whole genome shotgun sequence.
ACCESSION   DAORKP010000221 DAORKP010000000
VERSION     DAORKP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12752_length_2651_cov_7.319337"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1871
                     /locus_tag="PLN75_09195"
     CDS             54..1871
                     /locus_tag="PLN75_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG04331.1"
                     /translation="MLKNHPKGLLVAFFTNMGERFGFYTMMAILVLFLQVKYGLTEQA
                     AGEYYSWFYFAIYALALVGGMLADWSKKYKTVILWGQIIMFSGYVLIALPGMSLAGTM
                     AALLIISLGNGFFKGNLQAVVGQLYDDERYSKFRDAAFMIFYMGINIGAFFAPFVATG
                     IRNWWLESHGFNHDGSIPALAHRMIDGSLADTSQLQELANKAVISGQPVTDLTAFAHQ
                     YLDVFSKGYNLAFAVAAAAMIVSLLVYVIFNKALPSKSVAKAVEKQSNTIDNKSAHTG
                     RVLIVSLLLMAVTTFLIQLIPGLDYKFGLAIGLFVAFVSYIFQIATKEERPRVISLIL
                     VFIVVIFFWMSFHQNGLTLTFFARDYTVKEVGPFTNLFFNLESILCVIATILGVVLML
                     RSKSTLRYRVIGGILTVVFGAICYYFVSTADQSNPIAPEVFQSFNPLFIVSLTPVVMG
                     FFNYLNSKGKEPSTPRKIGFGMIIAGLGFVVILIASLNLVSPYLLEGKAVPDTSRVTP
                     YWLMSSYLILTIAELFLSPMGLSFVSKVAPPRFQGLMQGGWLLATAVGNKLLFIGSTL
                     WGRVDLYILWGVFFICCMISATFIFAMMKRLEKVTKSSN"
     gene            complement(1972..2568)
                     /locus_tag="PLN75_09200"
     CDS             complement(1972..2568)
                     /locus_tag="PLN75_09200"
                     /inference="COORDINATES: protein motif:HMM:NF039392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6266 family protein"
                     /protein_id="HRG04332.1"
                     /translation="MSGTVGNVVLSSTNGKNVVRSKAFNQKDANSEAQQMHRNVFKKT
                     CDEYTSLSPFIADGFPSRPQSQSCYNAFMAANLSNAVDKSGEFPVFDYSKMVLSKGSL
                     QPVEVLEASITQEGIQVNYQPLSENGGAKADDVVIAILKTVEGRVLSVKKPRGQSEED
                     SILFQVSNASAERILYMYMMVISADKTKASKSVYVSIA"
BASE COUNT          639 a          610 c          588 g          814 t
ORIGIN      
        1 gtcaggtgct tgcagttgta atggataatt aaataattaa atatcaatca gttatgttga
       61 aaaatcatcc aaagggatta ttagtggcat tcttcaccaa catgggcgaa cgattcgggt
      121 tctataccat gatggcgatt ctggtgctat tcttacaggt taaatacgga ctgaccgaac
      181 aggcagccgg tgaatactac agttggtttt attttgccat ttacgcactt gcgctggtag
      241 gcggtatgct ggccgactgg tcgaaaaagt acaaaacagt gattttatgg ggtcagatta
      301 tcatgttcag cggctatgta ctgatagcac ttccgggtat gtcgttagcc ggaactatgg
      361 cagcattgtt gattatttcg ctcggaaacg gattcttcaa aggaaatctc caggcagtag
      421 taggtcagct ttacgacgac gagaggtact cgaaattccg cgatgctgct ttcatgattt
      481 tctacatggg tatcaatatc ggtgctttct ttgctccgtt tgtagctacc ggtatccgca
      541 actggtggct cgaaagtcac ggtttcaatc acgatggaag catccccgca ctggctcacc
      601 ggatgattga cggcagcctc gccgacactt ctcaacttca ggaattagcc aacaaggctg
      661 taataagcgg ccagccggtg acagatctaa cggcttttgc tcaccagtac ctcgatgtgt
      721 tttccaaagg atataacctg gcatttgcag tggcagcggc tgccatgatt gtttctttgc
      781 ttgtatatgt tattttcaat aaagcacttc cttccaaatc agtggccaaa gcagttgaaa
      841 aacaatcaaa caccatcgac aataaatcgg ctcacaccgg tcgggtactt attgtatcac
      901 tccttttgat ggctgttacc acgttcctta tccagcttat tcccgggctc gactataaat
      961 tcgggcttgc catcggtttg tttgtagctt ttgtttccta cattttccag attgccacca
     1021 aagaagaacg cccgagagtc atatcactta tcctggtgtt tattgtggtg attttcttct
     1081 ggatgtcgtt ccatcagaac gggcttacgc tcactttctt tgcccgcgac tatacggtga
     1141 aagaagtggg tccgtttacc aacctcttct tcaacctgga atccattctg tgtgttatag
     1201 ctactattct gggtgttgta ctcatgctca ggagtaaatc cacgctccgt tatcgtgtga
     1261 ttggcggaat tcttacggtt gtattcggcg ctatctgcta ctattttgta agtactgccg
     1321 atcagagcaa tccgattgca cctgaagtgt tccagtcgtt caatccgctg ttcatcgttt
     1381 cgctcacacc tgtagtgatg ggttttttca actacctgaa ttcaaaaggc aaagaacctt
     1441 ccacaccgcg caaaattggt ttcggtatga tcattgccgg actgggattc gtagttattc
     1501 tgattgcatc gctcaacctg gtatcacctt atctgctcga aggtaaagca gtgcccgaca
     1561 cctcgcgtgt gacaccttac tggctcatga gcagctacct gatactgacc attgccgagt
     1621 tgtttctcag cccgatggga ctgtcattcg tttcgaaagt ggctcctccc cgcttccagg
     1681 gattgatgca gggcggatgg ctgctggcaa ctgccgttgg caacaagttg ctgttcattg
     1741 gaagtacact ctggggacgc gtggatctgt atatcctgtg gggagtattc tttatctgct
     1801 gcatgatttc tgctacattt attttcgcca tgatgaaacg gctggaaaag gtaaccaaat
     1861 caagtaatta aattgaaatc gattccgcaa ctgaaagttg ccggaattga ataaaaataa
     1921 caaagacact gcgcaggcgg tgtctttgtt gtttctgaga agtaaaaatc cttatgcaat
     1981 gcttacataa accgattttg aagctttggt tttgtcggca gaaatcacca tcatgtacat
     2041 gtacaaaatc cgttctgcgc ttgcgttcga tacctgaaat aggatagaat cctcttccga
     2101 ttgtcctcgc ggctttttca ccgacaatac cctgccttct acagttttca gtattgctat
     2161 caccacatca tcagctttgg ctcccccgtt ttccgaaaga ggctgataat ttacctgtat
     2221 tccctcctga gtaatacttg cttccagtac ttctaccggt tgcaaagaac ctttggagag
     2281 aaccatttta gagtagtcaa acaccgggaa ttcacccgat ttgtccacag catttgacag
     2341 gttggctgcc ataaatgcat tgtagcacga ttgactctga ggccgggacg gaaaaccgtc
     2401 cgcaataaaa ggtgacagtg atgtatattc atcacacgtc tttttgaata cgttccggtg
     2461 catctgttgt gcttcactgt tcgcatcctt ttgattgaat gctttggaac gtactacgtt
     2521 ttttccgtta gtggaactta atacaacatt tccaactgtt ccggacattt tcccagtcaa
     2581 ggggttttgg cttgttgcca tttttatagt tgtatccagc ttttaaagct tcgctacgtt
     2641 gcgtcactat c
//
LOCUS       DAORKP010000222         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12795_length_2648_cov_10.632472, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000222 DAORKP010000000
VERSION     DAORKP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12795_length_2648_cov_10.632472"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2648)
                     /locus_tag="PLN75_09205"
     CDS             complement(<1..>2648)
                     /locus_tag="PLN75_09205"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRG04333.1"
                     /translation="IFDTLNAKAALFAAGEAMHEAGRRVELMLSVTVTDKSGRTLSGQ
                     TIGAFLTSVSHADLLSIGLNCSFGARDLKPYLQEISKRVPVFVSAYPNAGLPNQFGEY
                     DETAEIMAAQVKEYFDERLVNIIGGCCGTTPAHIAAYQRLIEKAEVRTPAEKDTNLHL
                     SGLEEVVVLSPTVDFYKQSAVDKVQTSQQVHNDEAISGNSDLSPNPSPGERGIRPEYY
                     TTDANNWKLLHSISKDMRKNPTEAEDKLWQMLRKKSTGYKIKRQQVIDGYIADFICIE
                     KRVIIEVDGEIHLTPENIEKDKERSRILNAQGYDVLRFTNKYVIDNQIKVFDEIKSYL
                     SNKSTPSRRSLSFIEPRFPLSSGEGAGVEVEPASNFENLYSRQNAHSLLWEQGERSLF
                     LNIGERCNVAGSRKFLRLINEKKYEEALSIARKQVEDGAQVIDINMDDAMLDSKTEMV
                     TFLNYLASEPEIARVPVMIDSSKWEVIEAGLKCLQGKCIVNSISLKEGEEEFLEHARL
                     IKRYGAAVVVMAFDEKGQAADIERRIEICTRAYKLLTEKLNFPPQDIIFDPNVLAIAT
                     GIEEHNNYGVDFINSTKWIKENLPFAKVSGGVSNLSFSFRGNDPVREAIHSVFLYHAV
                     RAGMDMGIVNAGMLQIYEDIPAEMLEHVEDIVLNRRPDATERMIEFAEKIKNETTGVK
                     AEQVDAWRSGPLEKRLEYALIKGISDYMDEDIAEALTVYPSAISIIEQPLMNGMNIVG
                     ELFGAGKMFLPQVVKTARTMKKAVAILQPEIEKTKVPGQSSSAGKFLIATVKGDVHDI
                     GKNIVAVVLGCNNYEVIDLGVMTPTEKIIRTAIDEKVDIVGVSGLITPSLEEMCNVAL
                     EMEKAGLQVPLLIGGATTSKLHTAVK"
BASE COUNT          678 a          658 c          502 g          810 t
ORIGIN      
        1 attttcaccg ccgtatggag tttggaagta gtagctcctc caatcagaag tggcacttgc
       61 agtccggctt tctccatttc caaagccacg ttacacattt cttccaacga aggcgtaatc
      121 aatccactca caccaacaat gtccactttt tcgtcaatag cagtccggat gattttctcg
      181 gtcggtgtca tcacgcccag gtcaatcact tcataattat tgcatccgag caccactgct
      241 actatatttt ttccaatatc atgaacgtca cctttcaccg tagcaatcag gaacttgccg
      301 gcagaagaac tttgtcccgg aacttttgtt ttttcgattt caggctgaag tatcgcaacg
      361 gcttttttca tcgtgcgggc agtttttacc acttgtggaa ggaacatttt tccggcgcca
      421 aacaattcac caacaatatt cattccgttc atcaggggct gttcgataat cgaaatggca
      481 gatggataaa cggtcaaagc ttcggcaata tcttcgtcca tgtagtcgct gattcccttt
      541 atcagcgcgt attccaatcg tttttccaac ggcccggaac gccacgcatc cacctgctct
      601 gcttttacgc cggttgtttc gttttttatt ttttcagcaa attcaatcat ccgctccgta
      661 gcatccggac gacggttgag cacaatatcc tccacatgtt cgagcatttc ggcaggtatg
      721 tcttcataaa tctgaagcat cccggcgttc actatgccca tatccattcc ggctctgaca
      781 gcatgatata agaaaacaga atgaatagct tcgcgcaccg gatcatttcc acgaaaagag
      841 aaggataaat tacttacacc gccgctcact ttggcaaacg gcagattttc ttttatccat
      901 ttagtcgaat ttataaaatc tactccgtaa ttattatgtt cttcaattcc cgtagctatc
      961 gccagcacat tcgggtcgaa aataatatcc tgaggaggga agttcaattt ttcagttagt
     1021 agtttgtaag ctcttgtaca aatctcaatc cgcctttcga tgtcagcagc ctgccctttt
     1081 tcatcgaacg ccattaccac caccgcagcg ccgtaacgtt ttatcagtcg ggcatgttcc
     1141 aaaaattctt cttcgccttc tttcagactg atggaattga cgatgcattt cccctgcaaa
     1201 cacttcagtc cggcttcaat cacttcccat tttgaactgt caatcattac aggtacacgt
     1261 gcaatttcgg gttcggaagc caggtaattc aggaaggtaa ccatttccgt tttgctgtcg
     1321 agcatagcgt cgtccatgtt aatatcgata acctgagcac cgtcttccac ctgcttacgg
     1381 gcaatggaaa gggcttcttc gtatttcttt tcgtttatca accggaggaa tttccttgaa
     1441 ccggctacgt tgcaacgttc gccgatgttg aggaagaggg atctctctcc ctgctcccaa
     1501 agcagggaat gcgcattctg cctactatat aaattttcaa aattacttgc aggttcgacc
     1561 tctacccctg ccccctctcc cgaggagagg ggaaagcgcg gttcaatgaa cgataaagaa
     1621 cgacgagaag gtgttgattt attgcttaaa taggatttaa tttcatcaaa tacttttatc
     1681 tgattgtcaa ttacatattt atttgtaaaa cgcaagacat cataaccttg tgcattcaaa
     1741 atccgagatc gttctttatc tttttcaata ttttctggtg ttaagtgaat ttctccatca
     1801 acttcaatga ttactctttt ttcaatacaa ataaagtcag caatatatcc atcaataacc
     1861 tgctgtcttt taattttata accggtactt ttctttctca acatttgcca aagtttatcc
     1921 tcggcctccg ttggattttt gcgcatatct tttgaaatag aatgcaacaa tttccaatta
     1981 ttagcatccg ttgtgtaata ctcgggtctt atccccctct ccccgggaga ggggttaggg
     2041 gagaggtctg aatttccgga aatagcctca tcattatgaa cctgttgtga ggtttgcacc
     2101 ttatcaactg cagactgttt ataaaaatcc acggttggcg acaaaaccac tacttcctcc
     2161 aatccactca aatgcaaatt cgtatctttt tcagccggtg ttctgacttc cgctttttcg
     2221 attaaacgct gataggcagc aatatgggca ggtgtggttc cgcagcatcc tccgatgata
     2281 ttgaccagtc tttcatcaaa atattctttc acctgagcgg ccattatttc tgccgtttcg
     2341 tcgtattcac caaactgatt gggcaaaccc gcattgggat atgcactgac aaatacaggt
     2401 acccgttttg atatttcctg taaataaggt ttcaaatctc tggcaccaaa cgagcagttt
     2461 aatccaatgg aaagcaaatc agcatgcgat acggaagtca ggaaagcgcc aatcgtctga
     2521 ccggaaagtg ttctgccgct tttgtcggtc acggtaaccg aaagcatcag ttctaccctc
     2581 ctgccggctt cgtgcatcgc ttctccggca gcaaacagtg ctgcttttgc attcagcgta
     2641 tcaaaaat
//
LOCUS       DAORKP010000223         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12890_length_2638_cov_7.489353, whole genome shotgun sequence.
ACCESSION   DAORKP010000223 DAORKP010000000
VERSION     DAORKP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12890_length_2638_cov_7.489353"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..836
                     /gene="rimP"
                     /locus_tag="PLN75_09210"
     CDS             369..836
                     /gene="rimP"
                     /locus_tag="PLN75_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRG04334.1"
                     /translation="MISKDTVKQIVESYITAKGYYLVDINVTKDNHISVEIDHFEGVS
                     IDFCVELTREIESGIDRETEDYELQVSSAGLTEPFKVLKQYQKNTGNEVEVLTKEGKK
                     ITGVLTAADAEKITLQIEKQIKPEGAKRKVTLCEDLEIPYENIKTTKYIIRFK"
     gene            839..2092
                     /gene="nusA"
                     /locus_tag="PLN75_09215"
     CDS             839..2092
                     /gene="nusA"
                     /locus_tag="PLN75_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRG04335.1"
                     /translation="MAKKEHINLIDTFSEFKELKSIDRSTLISVMEESFRNVISKTYG
                     SDENFDVIINPDKGDFEIYRNRTVVPDDELENENVEISLSEAQKIDIDYEIGEEVTDT
                     VDFTAFGRRAILTLRQTLSSKILELQKDSLYTKYSEKIGEIVSAELYQIWKKEMLLMD
                     EDGNELYLPKSEQIPSDFYRKGETVRAVVAIVENKNNNPKIILSRTSPVFLQRLFELE
                     VPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGVKGARIHGIVRELRNEN
                     IDVINYTNNTNLFISRALSPAKITSIRLNEAEKKAEVYLKPDEVSQAIGKGGLNIKLA
                     SMLTGYTLDVFRDIDETENEEDIYLDEFSDEIDQWIIDTLKSIGCDTAKSVLAIPRED
                     LIRRTDFEEETIDEVLDILKSEFEE"
     gene            2224..>2638
                     /locus_tag="PLN75_09220"
     CDS             2224..>2638
                     /locus_tag="PLN75_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRG04336.1"
                     /translation="MSIRLSKAIKDLNVGLSTAVDFLAKKGHKVPSDINFKISDEEYL
                     LLAKEFNKDMALKLESERLSLERQSKEKAETVAIEGYQKKQPEKKEEVIKVTLSSDLR
                     PQIKEVGHIDLDKKPKTAPKPEEKPIVKEPEKPVAK"
BASE COUNT          967 a          501 c          500 g          670 t
ORIGIN      
        1 tattgcccca tcgtttcatt tttgtggctt ttctgtattc aaatgctctt cccataaatt
       61 gtttgagtta tttctttttt attcggtaaa aaaagaattt ccacattgaa attcatcctt
      121 ttttatccca cagttgtttt aattttagtc atcagaaagt cattttttgt tttttgaaaa
      181 gtgacttttt gaaggttttg caattttcag tttgcaaaat taggaaaata gcccgaaaaa
      241 tccataagtt tttgaatgaa tttcttcaat cctttgtaaa ttaaaaaatt attcgtaatt
      301 ttgcaccgaa atagaaaaac gtgagggggg acatacagtc cccttctttg ttgtaaaaaa
      361 accaatcaat gatttcaaaa gacactgtaa aacagatagt tgagtcatac attactgcca
      421 aagggtacta tctggtagat ataaatgtca caaaagacaa tcatatttca gtagaaatag
      481 atcactttga gggagtctca attgattttt gtgtagaact tacccgggaa atcgaatccg
      541 gaattgacag agaaacagaa gattacgagc ttcaggtttc ttcagcaggg cttacagagc
      601 ctttcaaggt actgaagcaa taccagaaaa acacagggaa cgaagtagaa gtgctcacca
      661 aagagggcaa aaaaatcacg ggcgttctta cagctgcaga tgcagaaaaa attacacttc
      721 agattgaaaa gcaaataaaa cccgaaggag caaaacgtaa agtcacgctc tgcgaagact
      781 tggaaatacc ttacgaaaat attaaaacaa caaaatacat tatcagattc aaataatcat
      841 ggctaaaaaa gagcatatca atttaattga tacattttct gaattcaaag agttgaaaag
      901 tattgacaga agtactttaa tcagcgtaat ggaagaatcg ttccggaatg taatttctaa
      961 aacatacgga agcgacgaaa attttgacgt cattataaat cctgacaaag gcgactttga
     1021 aatttaccgg aatcgtactg ttgttcccga cgatgaactg gaaaatgaaa atgtggaaat
     1081 ttcactttct gaagctcaaa aaatagatat cgattacgaa ataggcgaag aagtaactga
     1141 tacagttgac tttactgctt tcggacgtcg tgcaatactt acactccgcc agaccctttc
     1201 gtccaaaatc cttgagcttc aaaaagatag cctgtacacc aaatacagcg aaaaaatagg
     1261 tgaaatagtc agtgcagaac tctatcagat ctggaaaaaa gagatgttgc tgatggatga
     1321 agacggaaac gagctgtatc tgcccaaatc agagcaaata ccctccgatt tctaccgcaa
     1381 gggtgaaacc gtacgtgccg ttgtagctat cgtagaaaac aaaaacaaca atccaaaaat
     1441 catactctca cgcacttcac ccgtattcct tcaacgactg ttcgaacttg aagttcctga
     1501 aatacatgac ggattgatta ccattaagaa aattgcccgt attccgggtg aaagagccaa
     1561 agtagccgta gaatcatacg acgaccgaat tgatccggta ggtgcctgcg taggtgtaaa
     1621 aggtgcacga attcatggca tcgtacgcga actgcgcaac gaaaatatcg acgtaatcaa
     1681 ttacaccaac aacactaatt tattcatttc acgcgcactc agccctgcca aaatcacttc
     1741 aatccggctc aacgaagccg aaaagaaagc cgaagtatat ctgaaacccg atgaagtttc
     1801 gcaggcaata ggtaaaggcg gtctgaacat caaactcgcc agtatgctca ccggatacac
     1861 actcgacgta ttcagagata ttgacgaaac agaaaacgaa gaagatatct atctggacga
     1921 attctccgac gaaatagatc aatggattat tgacacgctg aaatccatcg gatgcgacac
     1981 ggcaaaaagc gttctggcta ttccgcgtga agatttaata cgtcgtacag attttgagga
     2041 agaaaccatt gacgaagtac tggatattct gaaatccgaa tttgaagagt aaccggaaat
     2101 aatcattgct tgtagtattc gtgaatattt caggctgaaa aacattaaaa ataattgatc
     2161 aggcggttaa ttacccgaag aacaattcag atcagaaaaa aacgtaaccc gtaaacaaac
     2221 aatatgtcta tcagattaag caaagctatc aaggatttga acgtgggatt atctactgca
     2281 gtagattttc ttgctaaaaa aggacacaaa gtaccttccg atattaattt caaaatttct
     2341 gacgaagaat atctgctgct tgcaaaagaa ttcaacaaag atatggcgct gaaattagag
     2401 tcggaacgac tgagcctgga acgtcaatcc aaggaaaaag ccgaaacagt agctatcgaa
     2461 ggttatcaga aaaaacaacc cgagaaaaag gaagaagtaa taaaagtaac tctttcaagt
     2521 gacctgcgtc ctcaaatcaa agaagtagga catattgatc ttgataaaaa gccaaaaaca
     2581 gctccaaaac ccgaagaaaa accaattgta aaagagcccg aaaaacccgt tgcgaaag
//
LOCUS       DAORKP010000224         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_12910_length_2635_cov_8.321318, whole genome shotgun sequence.
ACCESSION   DAORKP010000224 DAORKP010000000
VERSION     DAORKP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12910_length_2635_cov_8.321318"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLN75_09225"
     CDS             <1..672
                     /locus_tag="PLN75_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRG04337.1"
                     /translation="IHDVAVASPTSTVDINDSVSVTYLPKGSHQISYQVKDVRGVWSP
                     VVTDTISKTDNAFFSFTADKREISQNESVTFTPSIRHFIDSIVWNFGDGMTEVNFEPV
                     HTYDSIGHFDVTATVWHKGTLEGVSYVEIKYISVTPTGITTPEVSTIRMYPVPVSDML
                     TVESPDTPLRALRVVALNGTIVRQVTCRGENTSVISLGGLSPGTYIILTTTDKGVFSG
                     KVVKK"
     gene            838..1290
                     /locus_tag="PLN75_09230"
     CDS             838..1290
                     /locus_tag="PLN75_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04338.1"
                     /translation="MKKGNKWALMSLLLLANTLCHAQTAEFSTPEGYKWGMTKTEVLK
                     QNKGEPTKSENDFLYYLNTETMNSLLYKFSDNQLVAITKKYGFETENDLINDFEKYKK
                     QLTALYGANYKEISFETFEWIYRNTRIMLFNSYKDDKFKVFITYEKMK"
     gene            1335..2609
                     /locus_tag="PLN75_09235"
     CDS             1335..2609
                     /locus_tag="PLN75_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRG04339.1"
                     /translation="MDRQWFSFNRVFLVPILFFAFTTATSSQEISAQKNDSAFFCGIK
                     KEYAKGMSFSDFLQACLIQWNPVNNDSAKWEPFGVDISTQIRYSGFDEIIRKLARSSV
                     VNAYTASLTSADGRPIYCLEIGNGSKTVTFTAGVHAREVANPQFLLKFASELVSEYEK
                     GDTAIADLLTKVKLAVLPCVNPDGYEAATNGNGVIANKNLYLSAGNNREVFQTKSNAR
                     GVDLNRNFPSYSASLLWNEKKEETYLIKTYRNFYFFAGDTLGSENETKVTMNFLMKYI
                     PVSFRYVDFHSVGRSIFAGKPHLSDEFNKACLETGILISGITGYSLMGLDEENTGEGT
                     DGSLTDFAAEIASGFAYNPAIGRLAPPDSVNLVRKTEMFNYHCSVNTVETLNSLAKDK
                     KSNSLQVSTPPMHVDEWEKYHLYHLFLTLLKE"
BASE COUNT          850 a          550 c          526 g          709 t
ORIGIN      
        1 attcacgatg tggctgttgc ttcaccaacc tcaacggttg atataaacga ctcagtatcg
       61 gtcacttacc tgcccaaagg cagtcatcag ataagctatc aggtgaagga tgttcgcggt
      121 gtatggtcgc cggtcgttac cgatactatt tccaaaacgg acaatgcatt tttcagcttc
      181 acagccgaca aacgggaaat atcgcaaaac gaatcggtta cattcactcc ttccatccgg
      241 catttcatcg acagcatagt gtggaatttt ggcgatggca tgactgaagt gaactttgaa
      301 cctgtgcata cgtatgattc catcgggcat ttcgacgtta cagctaccgt gtggcacaag
      361 ggaactctcg aaggagtctc ttatgtggag attaaataca tcagcgtaac acccacaggc
      421 atcacaactc cggaagtcag cacaatccgg atgtatcctg ttccggtatc tgacatgctg
      481 accgtggaaa gtccggatac acctctcaga gctttacggg tagttgctct caacggaaca
      541 atcgttcgtc aggtcacctg ccggggtgaa aacacatcag tcatttcact tggcggactt
      601 tcaccgggca cctatatcat tcttaccacg accgataagg gtgttttttc cggtaaggtc
      661 gtgaaaaaat agtttcaatc cattgcccca cctccacaat agctatcgtg gaggcgtggc
      721 aactaaaaat gagttaggac gtcatgctga taaacattaa aattaataaa aagcatacag
      781 catgcgggtt aaaaaacatt tgataaaagc agaaacaaca actaaaaaac atccacagtg
      841 aaaaaaggaa ataaatgggc gctgatgagt cttttgctcc tcgccaatac gctgtgtcat
      901 gctcagacag ccgaattttc gacacctgaa ggttacaaat ggggaatgac aaaaactgag
      961 gtattgaaac aaaataaagg agagccgaca aaatctgaaa acgattttct ctactacctc
     1021 aacacagaaa caatgaacag cttgttgtac aaattttctg acaatcagct cgttgccatt
     1081 acaaaaaaat acggatttga aaccgaaaat gatcttatca acgatttcga aaagtataaa
     1141 aaacaattga ctgcactcta cggagcaaat tataaggaaa tttcttttga aacattcgag
     1201 tggatttacc gaaacacccg catcatgctg tttaacagct ataaagatga taaattcaag
     1261 gtttttatca catacgagaa aatgaaataa ttttttaaaa cttaatattt tcattaaaac
     1321 aggattaagt taaaatggat agacaatggt tctctttcaa cagagttttt cttgttccca
     1381 ttttgttctt tgcttttaca acagcaacaa gcagtcagga aatctctgcg caaaaaaacg
     1441 actctgcgtt tttttgcggc ataaaaaaag aatacgccaa agggatgtca ttttcggatt
     1501 ttttgcaggc ttgtcttatc caatggaatc ccgtaaataa tgactcggca aaatgggagc
     1561 ctttcggtgt tgatatcagc acgcaaatca ggtattccgg atttgatgaa ataattcgca
     1621 aactggcaag atcatccgtt gttaatgctt ataccgcatc attaacatcg gcagatggca
     1681 ggccaatata ttgcctggag atcggaaacg gctcaaaaac tgtgactttt actgccggag
     1741 tgcacgcacg cgaagttgcc aacccgcagt ttttgctcaa atttgcttcg gaacttgtat
     1801 cggaatacga aaaaggtgac actgcgattg ctgaccttct tacaaaagta aaactggctg
     1861 ttcttccctg cgtaaatccc gatggatacg aagctgcaac gaacggaaac ggggtaattg
     1921 caaataagaa tctatatttg tcagcaggta ataaccgcga agtttttcag acaaaatcaa
     1981 atgcaagagg cgtggacttg aatcgaaatt ttccttctta ttcagcttct ttgctatgga
     2041 acgaaaaaaa agaagaaaca tatctcataa aaacttacag aaacttttat ttttttgccg
     2101 gcgacacgtt gggcagtgag aacgagacaa aagttaccat gaattttttg atgaaataca
     2161 tccctgtttc ttttcgatat gttgattttc attcggttgg aagaagcatt ttcgccggaa
     2221 aaccgcattt gtccgacgaa tttaacaaag cctgcctgga aacgggaatc ctgatttcag
     2281 gcattaccgg atacagtctg atgggacttg atgaagaaaa caccggcgaa ggaaccgatg
     2341 gttcattaac cgattttgct gctgaaattg cttcgggttt tgcatacaat ccggccatcg
     2401 gacgacttgc accacccgat tcggtaaatc tggtaagaaa aaccgaaatg ttcaactatc
     2461 attgttctgt taacactgtt gaaacgttaa attcacttgc taaagacaag aaatcaaatt
     2521 cacttcaagt ttccacgcct ccaatgcatg tagatgagtg ggaaaaatac catttatatc
     2581 atttatttct aacattactc aaagaataaa caaacatccg ccattatgaa aacac
//
LOCUS       DAORKP010000225         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_13541_length_2563_cov_8.486045, whole genome shotgun sequence.
ACCESSION   DAORKP010000225 DAORKP010000000
VERSION     DAORKP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13541_length_2563_cov_8.486045"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..964)
                     /gene="ribF"
                     /locus_tag="PLN75_09240"
     CDS             complement(41..964)
                     /gene="ribF"
                     /locus_tag="PLN75_09240"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRG04340.1"
                     /translation="MFIYKNNDSVPWDKCVATVGFFDGVHGGHRFLLNEVNNRARTGQ
                     SISVAITFSEHPRKILKSDFQPRLLTTLEEKLLQLETTGLDACVVLNFTQQLAQLSAR
                     EFITQVLHERLHVKTLFVGHDHRFGHNREEGYEEYYNYGKALGMNVIRAEQFVLDGGE
                     HFSSSQIRKALEAGDILLANTLLTYPYTFLGKVIGGYKNGRKIGFPTANIWFDSKEKI
                     IPATGVYAVYVYLKGRKYKGMMNIGYRPTFSAGNSLSMEVHIIDFGADIYDHQINVEC
                     MYRIRNEMKFNGIDELVEQLKKDKQTVNNLL"
     gene            complement(1065..1709)
                     /locus_tag="PLN75_09245"
     CDS             complement(1065..1709)
                     /locus_tag="PLN75_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRG04341.1"
                     /translation="MTKYQHVTTVIFDLGGVIVDLDWELCIRNFENLGINNMHNLINT
                     TLQRGFILDYEMGQISDEQFREEIRKNATLKVTDEQIDYAWTSLLVGIPAEKLDLLME
                     LRKKYKVLMLSNTNNLSFRYSEKMFDVNGYRITDFFDKCYLSYEMKLAKPSAEIFVAL
                     LADAGLKAEECLFLDDGIHNINTASELGFQTEYIEPFSSVKDWEFPGKLIELNI"
     gene            1805..1879
                     /locus_tag="PLN75_09250"
     tRNA            1805..1879
                     /locus_tag="PLN75_09250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Ile,seq:cat)
     gene            2170..>2563
                     /locus_tag="PLN75_09255"
     CDS             2170..>2563
                     /locus_tag="PLN75_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRG04342.1"
                     /translation="MAEKRKIYKKSIHSIKEIAQNVFVLSFARNFEFRAGQVVAVDVV
                     TDGQPRLYSIASGEQDEYIEILFDEKPDGKLTPKLSALLPGDTIYVSEPFGNFFCNDE
                     SAWWLASGTGVAPFVSMVRSGQEIGKILI"
BASE COUNT          693 a          535 c          509 g          826 t
ORIGIN      
        1 ttttacaaaa ttattcatat ttttttatta ttaacgtaat ttacagcaaa ttgttgactg
       61 tttgtttatc ttttttcagt tgttcaacca gctcgtcaat tccgttgaat ttcatttcgt
      121 tgcgtatgcg gtacatgcat tctacattga tttgatggtc gtatatatca gccccgaaat
      181 caatgatatg gacttccatg ctgagtgaat tgccggccga aaaggtgggt ctgtatccga
      241 tattcatcat gcctttgtat tttcggcctt tcagatacac ataaacggca tatactccgg
      301 tggcgggaat aattttttcc ttgctgtcaa accagatatt ggcggttggg aatccgattt
      361 tccgtccgtt tttataaccg ccgattactt ttcccaaaaa cgtataggga taagtgagca
      421 atgtatttgc caggagaata tcacctgctt cgagcgcttt tcggatttgc gaactgctga
      481 aatgttcacc tccgtccagt acgaattgtt cggctcggat tacattcata cccagtgctt
      541 taccatagtt gtaatactct tcgtatcctt cttcgcggtt gtgaccgaag cggtggtcgt
      601 gtcccacgaa aagtgttttc acatgcaggc gttcgtgcag aacctgcgtg atgaattctc
      661 tggccgacag ttgtgccagt tgctgcgtga aattcaatac cacgcaggca tctagtccgg
      721 ttgtttcgag ttggaggagt ttttcctcca gggtggtaag gagtcgtggc tggaaatcgc
      781 ttttgagaat cttgcgcgga tgttcgctga aagtgattgc caccgaaatg ctttgcccgg
      841 tgcgcgccct gttattcact tcgttgagca aaaaccgatg gccgccgtgc actccgtcga
      901 aaaatcctac tgtagccaca cacttatccc agggtacgga atcgttgttt ttatagataa
      961 acactttttt atttacaatt tattgattta caatttacaa agccagaatt ttactgaaca
     1021 ggggttctca accactgata acctgacatt taactatttg actgttagat atttaattct
     1081 attaactttc cgggaaattc ccaatccttt acgctggaaa agggttcgat atattccgtt
     1141 tggaagccaa gttcgcttgc cgtgttgata ttgtgtatgc catcatccag aaacaaacat
     1201 tcttcggctt tgagccctgc atcggcgagc agtgccacga aaatttctgc agaaggttta
     1261 gcgagtttca tttcgtacga cagatagcat ttatcaaaaa agtcggtgat acgatatcca
     1321 ttcacatcaa acattttttc ggaatagcgg aatgacaaat tattcgtgtt gctaagcatc
     1381 aaaaccttgt atttttttct taattccatc agcaaatcca gtttttctgc cggtattccc
     1441 accaataacg acgtccaggc gtaatctatc tgttcgtcgg tcaccttcag ggtggcattt
     1501 tttcttatct cttccctgaa ttgttcgtcg gaaatctgcc ccatctcgta atcaagaata
     1561 aacccccgct gaagcgtagt gtttatgaga ttgtgcatat tattgattcc gagattttca
     1621 aaattccgaa tgcacagttc ccagtccaga tcaacaatca cacctcccaa atcgaatatt
     1681 accgtagtta cgtgctgata ttttgtcatt tttctaaaaa atatgaaata ttatttttgg
     1741 agttattcaa tttttattac ttttgcactc actttgcaaa gataatatat tttttgtaaa
     1801 tgtcgggccc atagctcagt tggttagtag cacctgactc ataatcaggg ggtcactggt
     1861 tcaagcccag ttgggcccac aaattaaaaa tcaaggtttt acacataatg tgtgaaacct
     1921 ttttttatct ttgttgaact attgttgaaa ctatgcagaa gcaaatgata ggatttttca
     1981 gtaaaaaact tcaaaaattc tacgttggag ttactcagca tgaaactata atgagatttg
     2041 acagatataa cttttttcaa ttttgaaaag aaccgaccca tatcaacttt catagattgg
     2101 agtctgtttt ttacaaccca tcattaaact caatttgatt tcgaatgttg tataaaaaac
     2161 tccgattcaa tggcagaaaa aagaaaaatt tacaaaaaaa gtatccactc aataaaagaa
     2221 atagctcaga atgtattcgt gctcagtttt gcgcgcaatt ttgaattcag agcaggacaa
     2281 gtagtggctg tcgatgtagt taccgacggc cagccacggc tttacagcat tgcaagcggg
     2341 gagcaggacg aatacatcga aattcttttt gatgagaaac ccgatgggaa gcttactccc
     2401 aaactttccg cattacttcc cggtgacaca atttatgtat ccgaaccatt cggtaacttt
     2461 ttctgcaacg acgaatcggc ctggtggtta gcttcgggca cgggtgtggc tccgtttgtt
     2521 tccatggtcc gttcgggaca ggaaatcggg aaaatcctca ttc
//
LOCUS       DAORKP010000226         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_13961_length_2519_cov_9.629464, whole genome shotgun sequence.
ACCESSION   DAORKP010000226 DAORKP010000000
VERSION     DAORKP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13961_length_2519_cov_9.629464"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLN75_09260"
     CDS             complement(<1..724)
                     /locus_tag="PLN75_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRG04343.1"
                     /translation="MKIKFIFPLFALLTVFFVSCYNSEDNIGVEIQPTADKITLKADT
                     FHLSSETFPVDRIVSQPDSLLLGTFIDDVLGTTRADILTQLALPTVNFTYLDESVATT
                     IPDSAVVTLSFNSYFGVSTSPVEISIYELKTKLKDKENYYSSIDPAAYVDFSKKLNAA
                     SEMLTVKDGLTGVQNTALSIKLSDEFLQRFFTKDPSVFASQANFENFFKGIYVTTDFG
                     SSVMVNVNNLSLTLYYHYVYKND"
     gene            complement(773..1588)
                     /locus_tag="PLN75_09265"
     CDS             complement(773..1588)
                     /locus_tag="PLN75_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRG04344.1"
                     /translation="MKEKKNTKILFISQEIFPYLPESDIANQSRYLPQAVQERGRETR
                     TFMPKFGLVNERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARLQIYFIDNDDY
                     FLRKNTVTDDNDKEYKDNDERSIFFVRGVMETVKKLRWTPDVIHCQGWMTAVAPLYIK
                     KAYNDDPFFKRSKVVYSLFDDEFKSPFNADFGKKLMLEGIEPRDIESVSGEVIDYEAV
                     SRLAIDFSDGVIQSHKTVKPELVEYTKSKGIKFLPFVETEDYVDEYIKFYDSL"
     gene            complement(1890..>2519)
                     /locus_tag="PLN75_09270"
     CDS             complement(1890..>2519)
                     /locus_tag="PLN75_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG04345.1"
                     /translation="HGRKFGTVGPPLPGVEVKLGERDEILCRGHNVMMGYYKDPVLTA
                     EVIDSEGWFHTGDTGKFTPEGQLIITGRLKSIFKTSFGKYVNPQAIESKFTESPFIEN
                     MIVVGENKKFAAALIVPDFVYLKSWCGIHKIDYTTNEEMIKHPEVTKRIMSEIKKYNA
                     LFGDYEQVKKYQLVADAWTMDNGFLSPTLKIKRKVIQNFYEKQIEKLFE"
BASE COUNT          731 a          529 c          472 g          787 t
ORIGIN      
        1 gatcgttttt atatacatag tgataataaa gtgtcagcga aagattattc acattcacca
       61 tcacggagct gccaaagtca gttgtaacat agataccttt gaagaaattt tcaaaatttg
      121 cctgactggc aaaaacagaa ggatcttttg tgaaaaaacg ctgcaggaat tcatcagata
      181 atttgatgga gagcgccgta ttttgcacac cggtcagtcc gtcttttact gtcagcatct
      241 cagaggcagc attgagcttt ttcgaaaaat ccacataagc tgccggatcg atgcttgaat
      301 agtaattttc cttgtctttc agctttgttt tcaattcata aatgcttatt tcaaccggcg
      361 aagtactcac tccgaaatag gaattgaaac tcagcgtaac tacagccgaa tcgggaatag
      421 ttgtagctac cgattcatcc agataggtaa agtttacggt aggaagtgca agttgtgtaa
      481 gaatatcagc tcttgtagta cccagcacat catcaatgaa ggttccgaga agcagcgagt
      541 cgggttgcga gacaatccta tctaccggaa aagtttcgga tgacaaatga aaagtatctg
      601 ctttgagagt aattttatcg gctgtaggtt gtatttcgac acctatatta tcttcactgt
      661 tgtaacatga gacaaaaaaa acagtcaata aagcaaagag aggaaatatg aatttaatct
      721 tcattcatca attaattaga agtaaaaaaa atttcagttt ctgagaaaaa agttacagac
      781 tatcgtaaaa tttaatgtat tcatccacat aatcttcggt ttccacgaaa gggagaaatt
      841 tgataccttt ggattttgta tattccacca gttcgggttt taccgttttg tgcgactgga
      901 tcactccgtc ggagaaatcg attgccaggc gtgaaaccgc ttcataatca atcacctcgc
      961 cgctaacact ttcaatatcc cggggctcta tgccttccag catcagtttt tttccgaaat
     1021 cagcattgaa aggcgacttg aattcatcgt cgaaaagtga ataaaccacc tttgacctct
     1081 tgaaaaacgg atcatcgtta tacgcttttt taatataaag gggcgctaca gctgtcatcc
     1141 agccctgaca atgaatgaca tcaggcgtcc agcgcagttt tttcaccgtt tccatcactc
     1201 cgcgcacaaa gaatatggat ctctcgtcgt tgtccttgta ttccttgtcg ttatcatccg
     1261 taacggtatt ttttctcaga aaataatcat cgttatcaat gaaataaatc tgcagacgtg
     1321 ccgactggat tgaagctact ttaatgatga gcggatgatc cgtatcgtca ataatcagat
     1381 tcatacccga aaggcgtatt acttcatgca actggtttct tcgctcgttt acgagcccga
     1441 atttcggcat gaaagtacgc gtttctctac ctctctcctg cactgcctgt ggcaaatatc
     1501 tactctgatt tgcaatatcc gactcaggta aatatggaaa aatttcctgc gaaataaata
     1561 aaattttggt attctttttc tccttcataa ttttgtctat ttacccaaag aaggtaataa
     1621 tatgcaaaga tataaaaaaa aaatctgata tacgcaagaa aaaaccgata actaacaatg
     1681 tttaacgaag ccgcacagtc gtaatttcaa gtatttaacc tcatcaattt acttttctga
     1741 cttctgaaaa atccttttta aaaaagagaa cggcttcctc ggatttcagg gaagccgcac
     1801 tcttttgctt cgcatttttc aatgcacaaa agtataatta ctgctgttaa tacgaaacta
     1861 ttttatactt ttttaattcg gctatgtgtt tattcaaaca atttttcaat ttgtttttca
     1921 tagaaattct gaatcacttt tcgctttatt ttcagcgtcg gcgatagaaa tccgttgtcc
     1981 atagtccacg catcggctac cagttggtac tttttcacct gttcgtagtc gccgaacaaa
     2041 gcattgtatt tttttatttc ggacatgatt cttttggtca cttccggatg ttttatcatc
     2101 tcttcgttgg tggtgtagtc tattttgtga atgccacacc aacttttgag gtacacaaaa
     2161 tccgggacaa tgagtgctgc ggcaaatttt ttgttttcac ccaccacaat catgttttca
     2221 ataaagggcg actctgtaaa cttcgattcg atggcctgcg gatttacgta ttttccaaaa
     2281 gaggttttga atatactttt cagtctgccg gtaatgataa gctgcccttc gggagtaaat
     2341 ttacccgtat cacccgtgtg gaaccaacct tcggaatcta taacttcagc ggtaagcacc
     2401 ggatccttgt agtagcccat cattacattg tgaccgcggc aaagaatttc atcgcgttcg
     2461 ccgagtttca cttccacacc gggcagcggc ggtcctacgg tgccgaactt ccggccgtg
//
LOCUS       DAORKP010000227         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_14024_length_2513_cov_7.439382, whole genome shotgun sequence.
ACCESSION   DAORKP010000227 DAORKP010000000
VERSION     DAORKP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14024_length_2513_cov_7.439382"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLN75_09275"
     CDS             <1..483
                     /locus_tag="PLN75_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HRG04346.1"
                     /translation="DPTEFKSTWLGNKSIYRTRMAIADGGELIVLAPALKEFGEDKEI
                     DRLIRKYGYFGTPATLKATSENKELQDNLGAAAHLIHGSSEGRFSITYCPGKGAENLS
                     RREIESVGFQWADIDEMMEKYNFNSLKEGFNTLPGGEEIFYISNPALGLWAHRDRFER
                     "
     gene            complement(543..1469)
                     /locus_tag="PLN75_09280"
     CDS             complement(543..1469)
                     /locus_tag="PLN75_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRG04347.1"
                     /translation="MEKKDIHIHSHGHDHGHIHTHHGDVKNIKIAFFLNLGFSIVEII
                     GGFLTNSVAILSDAVHDLGDSFSLGLAWYFQKFSRKQRDKNFTYGYKRFSLVGALINS
                     IVLIAGSVLILSEAIPRLFNPQQADAGGMFLLAILGIIVNGIAVVRLRKGSSLNEKVV
                     SLHMLEDVLGWSAVLIGAIIMKFTNFYIIDPILSVLISFFILFNVFKNIREVLRIILQ
                     GTPEQIKTDEIEATVREIEAIDNIHDLHLWTVDGTYNVLTIHVVLKTPQTMEQLSALK
                     TQIRDKLTQKGVEHITIEFETPDEECCMEDCC"
     gene            1652..>2513
                     /locus_tag="PLN75_09285"
     CDS             1652..>2513
                     /locus_tag="PLN75_09285"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRG04348.1"
                     /translation="MKRRTKKTAAVVLSVILLLVLTFAVLPFVFKDKVMEIARRELNK
                     RLTAEVNFRDLKISFIRSFPDASVSLKDLYIVGKDDFEGDTLLMSKDVNLVINLKSLF
                     GDTGYEVKRMQINDSKVLAHVLKDGRANWDIFPEDTANVDTTESGFKFKLQDFNIARA
                     DVVYLSDSGNVAARMKNLNLNLNGDLTADSTLLSTNFTIDTLNFWNGGVHYASNLVVE
                     FTADINAILSEDRYEFANNEMKINAIPLSLNGWVQMPDDEIEMDLTLNTEQVDFKSLL
                     SLIPAIYTNSF"
BASE COUNT          782 a          533 c          509 g          689 t
ORIGIN      
        1 gatccgactg aattcaaaag tacgtggctg ggtaataaat ccatttacag aacccgtatg
       61 gccattgcag atggcggaga actgatagta ctggctccgg cacttaaaga atttggcgaa
      121 gacaaagaaa ttgaccgcct gatacgtaaa tacggctatt tcggtactcc agccacgctg
      181 aaagcaacca gcgaaaacaa agaacttcag gataatctcg gagccgcagc tcaccttatc
      241 cacggttcgt ccgaaggaag attttccatt acttactgtc ccggaaaagg agcagaaaac
      301 ctctcgcgcc gggaaattga aagtgtaggt ttccaatggg cggatattga tgaaatgatg
      361 gagaaataca atttcaactc gctcaaagaa ggctttaaca cattgcccgg tggcgaagaa
      421 attttttaca tctccaatcc ggctttagga ctttgggcac atcgggatcg atttgagaga
      481 taaccgaaac aaaaaacagg actcaaccga atcctgtttt ttttatccct tcagtcagat
      541 tttcagcagc aatcttccat acaacactct tcatccggag tttcaaactc aatggtgata
      601 tgttccactc ctttttgtgt caatttgtcg cgaatctgag ttttcagtgc tgaaagttgc
      661 tccatagttt gtggagtttt cagcacgacg tggatggtca gcacattgta agtgccatcc
      721 acagtccata aatgcagatc gtgaatgttg tcgatggctt caatttccct gacggttgct
      781 tcaatttcat ccgttttaat ctgctcaggt gtaccctgca gaataatgcg gagcacttca
      841 cggatatttt tgaaaacatt aaaaagaata aaaaaggata tcaacaccga tagtatcgga
      901 tcgatgatgt aaaagttggt gaatttcata ataatggcac caatcagcac tgcactccat
      961 ccgagtacat cttcaagcat gtgcagcgaa accacttttt cattgagcga actccctttg
     1021 cgtagtctga cgacggctat tccgttcaca atgattccca gaatagccag cagaaacata
     1081 ccaccggcat cagcctgctg cggattgaaa agacgtggaa tagcttcgct cagaatgagc
     1141 acgctgcctg ctatgagtac aatggaattt atcaatgccc cgaccagcga aaaccgtttg
     1201 taaccataag tgaagttctt atcccgttgt tttcttgaaa atttctgaaa ataccaggcc
     1261 agtccgagcg aaaaactgtc gcccaaatca tgcacagcat ctgaaagaat tgccacacta
     1321 ttggtcagaa agcccccgat aatctcgaca atcgaaaaac ccagattcag aaagaatgca
     1381 atcttaatat ttttcacatc gccatgatga gtatgaatat gtccatgatc atgcccgtgt
     1441 gaatgaatat gtatgtcttt tttctccaat tttttctccg ttataatttt tcaattcagg
     1501 catcggaccc gaataatttt ccaaaaatac aaaaaaatat catttgctgt acagctttta
     1561 agtacgattt ttgttaacgt atcattcggg atttcattta aaaatatcta tttttgtaaa
     1621 tcattcattt tttacacaat ccgaaatatg tatgaaacga agaactaaaa aaacagcggc
     1681 agtggtactt tcagttattt tgttgctggt tctcacattt gcagtacttc catttgtttt
     1741 caaagataaa gtaatggaga ttgcgcgtcg cgaactgaat aaacgactga cagctgaagt
     1801 gaatttcaga gatctgaaaa tcagctttat acgaagtttt ccggacgctt cggttagcct
     1861 caaagacctt tatatagttg gaaaagatga ttttgaaggc gatacgctgc tgatgagcaa
     1921 ggatgtgaat ctggtaataa acctgaaaag tctttttgga gataccggtt acgaagtgaa
     1981 aagaatgcaa ataaacgact cgaaagtgct tgcacacgta ttgaaagatg gtcgggctaa
     2041 ttgggatatt tttccggaag atacggcaaa cgtggataca acggaatccg gcttcaaatt
     2101 caaattacag gatttcaaca tcgcccgggc cgatgtcgtt tatctgagcg attcgggcaa
     2161 tgtagccgca cgaatgaaga acctgaatct caatctgaat ggcgacctaa ctgcagatag
     2221 cacattactc tcaacaaatt ttaccataga tacactcaat ttctggaacg gaggtgtaca
     2281 ttacgccagc aatcttgtag tagaattcac agccgacatc aacgcaattc tcagcgaaga
     2341 caggtatgaa tttgcaaata acgaaatgaa aatcaatgca ataccactct cgttgaacgg
     2401 ctgggtacaa atgcccgacg acgaaataga gatggatttg acactcaata ccgaacaagt
     2461 cgatttcaaa tcgctgcttt cgcttattcc ggccatttat accaactcat ttg
//
LOCUS       DAORKP010000228         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_14376_length_2481_cov_9.934872, whole genome shotgun sequence.
ACCESSION   DAORKP010000228 DAORKP010000000
VERSION     DAORKP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14376_length_2481_cov_9.934872"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..99
                     /locus_tag="PLN75_09290"
     CDS             7..99
                     /locus_tag="PLN75_09290"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04349.1"
                     /translation="MGYRYGLFNYATKTTGGYVDFDYFNINYNN"
     gene            153..1832
                     /locus_tag="PLN75_09295"
     CDS             153..1832
                     /locus_tag="PLN75_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG04350.1"
                     /translation="MTLNPLVEMKALKKQVPNIPVFTSYVYQGEDQVYENNPLKPDEF
                     YNPILQGCYPDPSITRKGDDYYLVTSTFAMFPGVPIFHSKDLVNWTQIGHVLDRKSQL
                     KVQDTGISAGIYAPSIKYNANNETFYMITTQFAGDINNMVVKTKDPFQGWSEVYKLKF
                     NGIDPSLFFDDNGKAYVVHNDAPDRGKELYNGHRVIKIWEYDVENDQVIAGTDKIIVD
                     GGVDISRKPIWIEAPHIYKKNGIYYLMCAEGGTGDWHSEVIFTSENPMGPYVPAPNNP
                     ILSQRYLNSNRANKVDWAGHADLVEGTDGKHYGVFLAIRPNEKGLVNTGRETFILPVD
                     WSGKYPVFENGLVPMEPKLKMPKGVENKTGTEGFFPNGNFTFTDNFTAPKLDYRWIGM
                     RGPREDFITTSKNGLNIKPFAVSIKAVAPVSALFQRQQHQHFTATTTMKFTPKSENEL
                     AGLTCYQSERFNYVFGITKKDKDFYILLEKTEKGQSKIIASAKIDVKMPVQLQVTAKG
                     DEYKFNYSVDGVNFTNLGGIVSGDILSTNVAGGFTGSLIGLYATSANDAKP"
     gene            1896..>2481
                     /locus_tag="PLN75_09300"
     CDS             1896..>2481
                     /locus_tag="PLN75_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRG04351.1"
                     /translation="MYKRNFITGIILLLFLSVSVNGQNNIKVTKLKALKDAYTGKFLI
                     GTASDLRGFSDAELANIKMHYNAITPENSMKPQSLHPSMDKYNFGIADSLVDWSTKNG
                     LKVWGHTLLWHAQTAPWFFQANPELEETPRPRPAFQPNQPRAPRDPNAPRPSMAEMWL
                     RSVNGPLACRDVALERLKNHIMTVAGRYKGRVIGW"
BASE COUNT          769 a          541 c          561 g          610 t
ORIGIN      
        1 cactttatgg gctatcgtta cggtttgttc aattatgcca ctaaaaccac aggcggttac
       61 gtagatttcg actatttcaa catcaattac aacaactaaa tatcatgaga aagataaaaa
      121 aaaacttatt tctgctaagc ctgattgggt taatgaccct gaatccattg gtggaaatga
      181 aagcgctgaa gaagcaggta cccaacattc cggtattcac cagttacgtc tatcagggtg
      241 aagatcaggt gtatgaaaat aatccgctga agccggatga attctacaat cccattttgc
      301 agggctgtta tcccgacccg tccattaccc gaaaaggcga tgattattac ctggttacat
      361 ctacttttgc catgttcccg ggagtgccaa ttttccactc caaagatttg gtgaactgga
      421 cacaaattgg tcatgtgctc gacagaaaat cgcagctgaa agtgcaggat acaggcatca
      481 gtgccggaat ttacgctcct tccatcaaat acaatgctaa taatgaaacg ttctacatga
      541 ttaccacgca gtttgccggc gacatcaaca acatggtggt aaaaacaaaa gatccgtttc
      601 agggctggag tgaagtttac aagctaaaat tcaacggaat tgacccgtcg ctcttcttcg
      661 acgacaacgg aaaagcctac gtggttcaca acgatgcgcc ggaccgtgga aaagaactct
      721 acaacggtca ccgcgtgatt aaaatctggg aatacgatgt ggaaaatgac caggttattg
      781 ccggaacgga taaaattatc gtggatggcg gtgtggatat ttccagaaaa ccaatctgga
      841 tcgaagctcc gcacatttac aaaaaaaacg gcatttacta cctgatgtgt gccgaaggtg
      901 gaacaggcga ttggcacagc gaagtgattt ttacgagcga aaatccgatg ggaccctacg
      961 ttcctgctcc gaacaacccg attttatcgc aacgctacct caactcaaac cgcgccaaca
     1021 aggtggattg ggcaggtcat gccgatctgg tggaaggtac tgacggaaaa cattacggtg
     1081 tatttctggc cattcgcccg aatgaaaaag gactggtaaa caccggtcgt gaaacgttta
     1141 tcctccccgt ggactggagc ggcaaatatc ccgtgttcga aaacggactg gtacccatgg
     1201 aaccgaagtt gaaaatgccg aaaggtgtgg agaataaaac cggaacggaa ggattttttc
     1261 ccaacggaaa tttcactttt accgacaatt tcacagcgcc gaaactcgat taccgctgga
     1321 ttgggatgag aggtccgcgt gaagatttca tcaccacctc taaaaacgga ttgaacatca
     1381 aaccttttgc tgtaagcatc aaagcagttg caccggtttc tgcacttttt cagcgccaac
     1441 agcaccagca ctttaccgcc acgactacga tgaagtttac tccaaaatcg gaaaatgaac
     1501 tggccggatt gacctgctac caaagcgaac gattcaatta tgtgtttggt ataacgaaaa
     1561 aagacaaaga cttttacatt ctgcttgaaa aaacggaaaa aggacaatcc aaaatcattg
     1621 caagcgcaaa aattgatgtg aaaatgccag ttcagctgca ggtgacagcc aaaggcgacg
     1681 agtataaatt caattattct gttgacggcg tgaatttcac gaatctgggt ggcattgtgt
     1741 caggcgatat cctctcaacc aacgtagccg gcggatttac cggaagttta atcggactgt
     1801 atgctacaag tgccaatgat gctaagcctt aacaagatat gagataaaaa agtgtgatta
     1861 ttcggaacta atttaaaatt ctaaaaaata aaattatgta caaaagaaac tttattacag
     1921 gcattattct gctgttgttt ctgtctgtaa gtgtaaatgg gcagaacaat atcaaagtaa
     1981 caaaattaaa agcgttgaaa gatgcttata caggaaaatt cctgattgga actgcaagtg
     2041 atttgagggg attctctgat gctgaattgg caaatatcaa gatgcattac aacgctatca
     2101 ctcctgaaaa ctccatgaaa ccgcagtcac tccatccttc aatggataaa tacaactttg
     2161 gaattgctga ttctttggtt gactggtcca cgaaaaatgg tttgaaggtt tggggacaca
     2221 cgttgctgtg gcatgctcaa acggcaccct ggttttttca ggccaatcct gaactggaag
     2281 aaacgccgag accaaggcct gcttttcagc cgaatcagcc cagagctcca cgggatccca
     2341 atgctccccg ccccagcatg gctgaaatgt ggttgcgaag tgtcaacgga cctctggcat
     2401 gtcgtgacgt tgctttggaa cgtttgaaaa accacattat gacggtagcc ggacgttata
     2461 aaggccgcgt aatcggatgg g
//
LOCUS       DAORKP010000229         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_14426_length_2475_cov_9.192975, whole genome shotgun sequence.
ACCESSION   DAORKP010000229 DAORKP010000000
VERSION     DAORKP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14426_length_2475_cov_9.192975"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /gene="folE"
                     /locus_tag="PLN75_09305"
     CDS             complement(<1..515)
                     /gene="folE"
                     /locus_tag="PLN75_09305"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRG04352.1"
                     /translation="MISFDQEKTLLLAEKYKEILDILGENSDREGLLKTPQRVAKAMQ
                     FFMQGYEQDPEHILRSAMFTEDYRQMVIVKDIDFYSLCEHHLLPFFGKAHVAYIPNKK
                     ITGLSKIARVVEVFARRLQVQERLTTQIKECIQQTLDPMGVIVVIEAQHLCMQMRGVQ
                     KQHSITTTSDFT"
     gene            complement(546..1022)
                     /locus_tag="PLN75_09310"
     CDS             complement(546..1022)
                     /locus_tag="PLN75_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRG04353.1"
                     /translation="MMNKFFSITGIMLFGIHFLIAQTPEKSARPAVIENIENNSKVVI
                     NQDPKLDKLITDHINRKDESASGAYTGYGYRVQVFSSNAQKTAKSEAFSIERKLRSAF
                     PGYGVYRIYASPFWKVRIGDFRTLEDAKAFRSEVVKAFPELSRETYTVRESKVSIR"
     gene            1180..1842
                     /locus_tag="PLN75_09315"
     CDS             1180..1842
                     /locus_tag="PLN75_09315"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRG04354.1"
                     /translation="MNKTIIIVAGGKGERMNSQVPKQFLVLAGKPVLMHTIERFHRYS
                     DKMDILLVLPSTQIEYWKELCLKHDFGIPHRIVEGGIARFFSVKNALALIQNSGLVGV
                     HDGVRPLVDEPTIERCFDEAEKSGAAIPVTDTVESIRRISENGSKAVDRNEYKMVQTP
                     QVFRAEILLKSYTQPYNELFTDDASVAEAAGYTVKLVAGNRENIKITTPYDLKLAESM
                     LS"
     gene            1850..>2475
                     /locus_tag="PLN75_09320"
     CDS             1850..>2475
                     /locus_tag="PLN75_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRG04355.1"
                     /translation="MEEKKQRIEWLDSLRAIAIIAVILVHVSSPVVNMAYGRNMSHWW
                     IGNIFDSAVRFAVPLFLMISGAMLLSATYDIKTFYKKRFIRVLIPFLFWMVIYWIFRY
                     LTLPGSQPHGFHDIISWAVNIFMKEGISKHFWFVYMIIFVYLITPFIGMFVRKLKPEM
                     LVFLLAAWVLIVSISKDFAIDMYGWRGNLLYKFFMYFLYSGYMVLGYY"
BASE COUNT          700 a          509 c          535 g          731 t
ORIGIN      
        1 gtaaaatcgg atgtggtagt gatggaatgc tgtttctgca cgccgcgcat ctgcatacaa
       61 agatgctgag cttcaatgac aacgataaca cccatcggat cgagcgtttg ctgaatgcat
      121 tctttaattt gggtggtaag gcgttcctgc acttgcagcc ggcgtgcaaa aacctccaca
      181 acacgggcta ttttgctcaa tccggtgatt ttcttattcg gaatataagc cacatgagcc
      241 ttaccgaaaa acggtagtaa atgatgctcg caaagcgaat agaaatcgat atctttcaca
      301 atgaccatct gccggtaatc ttcggtaaac atggccgaac gcagaatatg ttccggatcc
      361 tgctcgtagc cctgcataaa aaactgcatg gctttggcta cccgctgagg agttttcagc
      421 aaaccttcac ggtcagaatt ttcgcccaga atatcaagaa tttccttgta tttttcagct
      481 aacaagaggg ttttttcctg atcaaaagaa atcagattgt tttcgtagag gtccatttat
      541 tcagtttatc ggatggatac tttgctttct ctcacagtat atgtttcgcg actcaattca
      601 gggaatgctt tcactacttc cgagcgaaat gccttggcgt cttccagtgt tctgaaatcg
      661 ccgattctca ctttccagaa aggagatgca taaatcctgt atacaccgta tccgggaaaa
      721 gcacttctta gttttcgttc aatggaaaaa gcttcgcttt tggcagtctt ctgcgcatta
      781 ctcgaaaaaa cctgaaccct gtatccataa cccgtataag caccggaagc cgattcgtcc
      841 ttgcggttga tgtggtctgt gatcagctta tcgagttttg gatcctgatt tatgacaact
      901 tttgaattat tctctatgtt ctcaataaca gccggtcggg ctgatttttc aggtgtctgt
      961 gcaatcaaaa aatgaatgcc gaaaagcatt attccggtta tggaaaaaaa cttattcatc
     1021 atgttttgag tttatatttt ttacaaatat atgaaaataa aacaattttt agccggtttt
     1081 ttaaaaaaaa caattgcatt aactattatt tcaaaaccac aaaagttgaa tttttcagtc
     1141 aaatacagta tctttgttgg aaatagctga cctatgaata tcaataaaac catcatcata
     1201 gtagccggag gaaaaggaga gagaatgaac tcgcaggttc cgaagcaatt tctcgtactg
     1261 gccggaaaac ccgttctgat gcataccatt gaacgttttc accgctactc tgataagatg
     1321 gatatcctat tggtattgcc ctcaacacaa attgaatact ggaaagagct gtgtctgaaa
     1381 cacgatttcg ggattcctca ccggattgtc gaaggaggta tcgcacgatt tttttcggtg
     1441 aaaaatgccc tggctttgat ccaaaactcc ggacttgtgg gagtacacga cggagtaaga
     1501 cccctcgtgg atgagcccac gatagaaaga tgctttgatg aagccgaaaa atcgggtgct
     1561 gccattccgg tgaccgatac cgtggaaagt atccggagaa tctcagaaaa cggaagcaag
     1621 gctgtggaca ggaatgaata taagatggtg cagacacctc aggttttcag agccgaaatt
     1681 ctgctgaaaa gctacaccca gccttacaat gagctattta ccgacgacgc ctcggtagcc
     1741 gaagccgccg gttacactgt aaaactggtg gcaggcaaca gagaaaatat caaaattaca
     1801 actccttatg acttaaaact ggcagaatcc atgctatcat agtgcattta tggaagagaa
     1861 aaaacaaaga attgaatggc tggacagcct gcgtgccatt gctattatcg cagtaattct
     1921 ggttcatgtt tcatcgccgg tggtaaatat ggcttacggt cgcaacatgt cgcactggtg
     1981 gatcgggaat atatttgaca gtgcggtaag gtttgccgtg ccgctttttc tgatgataag
     2041 cggagccatg cttctttcag ccacatacga catcaaaacg ttttacaaaa aaagatttat
     2101 ccgcgtactg ataccttttc tgttttggat ggtaatttac tggattttca ggtatttaac
     2161 cctgccgggc agccagccgc acggatttca tgatatcata agctgggctg tgaatatttt
     2221 catgaaagaa ggtatctcta agcatttctg gtttgtttat atgattattt tcgtctatct
     2281 gattactccg tttatcggca tgtttgtcag aaagctgaaa cccgaaatgc ttgtttttct
     2341 cctggctgcc tgggtgctca ttgtaagcat aagcaaagat tttgccatcg atatgtatgg
     2401 atggagagga aacctgctat acaaattttt catgtatttt ctgtattccg gttacatggt
     2461 gttgggttat tattt
//
LOCUS       DAORKP010000230         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15028_length_2419_cov_8.181049, whole genome shotgun sequence.
ACCESSION   DAORKP010000230 DAORKP010000000
VERSION     DAORKP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15028_length_2419_cov_8.181049"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLN75_09325"
     CDS             <1..191
                     /locus_tag="PLN75_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRG04356.1"
                     /translation="FKLFLKHSDILERVTLWGVYDGQSWRNNWPVRGRTDYPLIFDRN
                     FQPKPVVKFIVEEAETTK"
     gene            472..1449
                     /locus_tag="PLN75_09330"
     CDS             472..1449
                     /locus_tag="PLN75_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG04357.1"
                     /translation="MKQPKYLINQPDHYMADPSAHVFNGKIYIYPSHDVEAGIPENDN
                     GDHFDMRDYHVFSMEDIDGEVIDHGVVLSVKDIPWAGRQLWDSDVAEKDGKYYLYFSL
                     KDKNDVFHIGVATSHRPEGPFVPESNHIPGSYSIDPCAFTDEDGACYLYFGGLWGGQL
                     QRYRNNKAIECGHEPADDEPALCAKVAKLSENMLGFAEEPRDVLIVDKDGTPLKAGDH
                     DRRFFEASWMHKYNGKYYFSYSTGNTHFLCYAIGDNPYGPFVYQGVILTPVVGWTTHH
                     SIVEIKGKWYLFHHDSVPSGGRTWLRSMKVVELEYNDDGTIKTIDGGAE"
     gene            1530..>2419
                     /locus_tag="PLN75_09335"
     CDS             1530..>2419
                     /locus_tag="PLN75_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HRG04358.1"
                     /translation="MKKIAFCFLVVFINLFVVHAEDGHRLWLRFNTSQEGVHHFSGLM
                     GDNNVLAINEFQEAWKLLTGSYLPANKALTDKVLVIGTSKNRTIKSFGFEKTLKSLGN
                     EGYILKSVNKKNKIYTVVTANSDAGLLYGVYHLLRLIQTSQFDENLNIIEKPAYNHRL
                     LNHWDNLDRTVERGYAGYSIWRWDELPAKISPLYKAYARANASVGINGTVLNNVNASP
                     QILSKEYLEKVKIIAGELRPYNIKTYLSINFSSPKELGGLKDSDPMNPEVQKWWKEKA
                     KEIYAMIPDFGGFLVKANSEG"
BASE COUNT          760 a          495 c          515 g          649 t
ORIGIN      
        1 ttttcaaatt attcctgaaa cacagcgata tattagaacg tgtaacgctc tggggtgttt
       61 acgacggaca atcctggaga aacaactggc cagtaagagg cagaaccgat tatccgttga
      121 tttttgaccg aaatttccag ccaaaaccag ttgtgaaatt catcgttgaa gaagctgaaa
      181 cgacaaaata aaaccaagga aagagatttt tttataaccc gatatttata aacgcattat
      241 gttttatttt cgccataagc gatgtatatc aatagtaaaa cggttaattc aaaaatcatt
      301 tttccataga caacagacaa atttatttat tccctgcggg aaaacagaat aacctgcatc
      361 attttttatt gatataaaag cactaacgta ctaaaaaaaa caacttaaca caacagaact
      421 taacaaccct gccccattag gaacgatata ggcagcaata aaattatatt tatgaaacaa
      481 ccaaaatatt taattaatca acccgatcac tacatggctg atccctctgc ccatgttttt
      541 aacggcaaaa tttatattta tccatcacac gacgtagaag ccggaatacc ggaaaacgac
      601 aacggtgatc attttgatat gcgagattat cacgttttct caatggaaga tattgatggt
      661 gaggtgattg atcatggcgt agtgctgagc gtgaaagata ttccatgggc cggccgccag
      721 ctgtgggatt cggatgtggc ggaaaaagat ggaaaatact acctctattt ttcgctcaaa
      781 gataaaaacg atgtatttca catcggagtt gccacgagcc acagacccga aggaccgttc
      841 gtgcccgaaa gtaatcacat tcccggaagc tattccatcg atccctgtgc ttttaccgac
      901 gaagacggtg cctgttacct ctatttcggt gggctctggg gcggacagct tcaacgctac
      961 cgaaacaaca aagccatcga atgcggtcat gaaccggctg atgatgagcc tgctttgtgt
     1021 gcgaaagtgg caaaactgag tgagaatatg ctcggatttg ccgaagaacc acgcgatgtg
     1081 ttgattgttg ataaagacgg cactcctttg aaagccggag accatgaccg gcgctttttt
     1141 gaagcatcgt ggatgcacaa atacaacgga aaatactact tctcgtactc taccggaaat
     1201 acccattttc tttgctacgc aatcggcgat aatccttacg gaccgtttgt atatcagggc
     1261 gttattctga ctcctgttgt gggttggact acacatcatt ccattgtgga aataaaagga
     1321 aaatggtatc ttttccatca tgacagtgta ccttcgggcg ggcgtacctg gttgcgaagc
     1381 atgaaagtgg ttgaactcga atacaacgat gacggaacga ttaaaacgat tgacggcgga
     1441 gcagaataat ccggattttt cactcaaatg cagcttgcag aaatttttat ggctgctgca
     1501 ggagtataat cttatttcaa cgaataataa tgaaaaaaat agcattttgt tttctcgtag
     1561 tttttattaa tctttttgta gttcatgctg aagatgggca ccgactgtgg cttcgtttta
     1621 atacaagtca ggaaggtgtc catcacttca gcggtctgat gggcgataac aatgtactgg
     1681 ccatcaacga atttcaggaa gcctggaagt tgttaaccgg aagttatctg ccagctaaca
     1741 aagcacttac agataaagtg ctggtgattg gtacttcgaa aaacagaact atcaaatcat
     1801 ttggattcga aaaaacgctc aaatcgcttg gaaacgaggg ctatatttta aagtcggtta
     1861 ataagaaaaa caaaatctat acggttgtaa ctgcaaattc tgatgccgga ttgttgtacg
     1921 gagtatatca tttgcttcgc ctgattcaaa ccagtcagtt tgatgaaaat ctcaacatca
     1981 ttgaaaaacc ggcttacaac catcgtttgc tcaatcactg ggataacctg gatcgtactg
     2041 ttgagcgcgg ttacgccggt tattccatct ggcgctggga cgaacttcct gcaaaaatat
     2101 caccgcttta caaagcatat gcacgggcaa atgcatccgt cggaatcaac ggaacggtgc
     2161 tcaacaacgt gaatgcttcg cctcagattt tgtcaaaaga atacctggaa aaagttaaga
     2221 ttattgccgg cgaacttcgt ccctacaaca tcaaaaccta tctttccatc aatttttcat
     2281 cgcccaaaga gcttggcgga ttgaaagatt ccgatccgat gaatccggaa gtacagaaat
     2341 ggtggaagga aaaagcaaag gaaatctatg cgatgattcc cgatttcgga ggatttctgg
     2401 tcaaagccaa ttcagaagg
//
LOCUS       DAORKP010000231         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15030_length_2419_cov_6.937817, whole genome shotgun sequence.
ACCESSION   DAORKP010000231 DAORKP010000000
VERSION     DAORKP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_2419_cov_6.937817"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="PLN75_09340"
     CDS             <1..1619
                     /locus_tag="PLN75_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRG04359.1"
                     /translation="FESGTRDWHREIQQAIETVATISGAQLIDFFEPMYPYHQLLHDA
                     VHPNAEGARILAQTVYSSITGDFGGLKMPAIYTDNMVLQRNRPLRITGTANAGEKITL
                     TIGKQKHKTTTADNGKWTVTINPLTAGKPYTLTVTSPQNKLTFQNVLAGEVWLCSGQS
                     NMEFRLSRSFESKKDIQSAANPEIRLFDMKARWLTNDYTWSPAALDSVNRLQYFTQPR
                     WEECTPENAAAFSAVAYYFGKMLQDSLRVPVGLICNAVGGSPAESWVDRRELENHFPA
                     IFRNWTENDFIQDWVRTRAKSNMGKDAPKLQRHPYEPGYLFDAGIRPLAQYPIAGAIW
                     YQGESNAHNKDAHAKLFKLLIESWRENWENDSLPFYFVQLSSINRPSWTWFRDSQRRL
                     QAEIPFTGMAVSSDLGDSLDVHPIHKKEIGERLARIALYNTYGKTAVVPSGPFFREVN
                     FKRKEAIVTFDYAEGLQTSDGKSPQTFEIAEFPGFFFPAKAKITGNQIQLTAPEVKNP
                     KFVRYGWQPFTRANLINAAELPASTFTTEE"
     gene            1693..>2419
                     /locus_tag="PLN75_09345"
     CDS             1693..>2419
                     /locus_tag="PLN75_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRG04360.1"
                     /translation="MKNSKYYPWIVVGLLWIVALLNYLDRQMLSTMQKSMMIDITELA
                     IAENFGRIMGIFLLIYGLMSPVAGVIADRINRKWLIVGSLLVWSAVTYGMGYAKTYEQ
                     VYWLRALMGVSEALYLPTGLAMIADFHSSKTRSLAIGIHMSGLYTGQALGGFGATIAA
                     NFSWQAVFHLFGIIGIIYAVVLIFLLHDKEGHAGTKTANLQPAALRSTKEPFMKSFGV
                     IFGMLSFWILLFYFMAPSFPGWAT"
BASE COUNT          663 a          606 c          567 g          583 t
ORIGIN      
        1 gctttgaatc gggcacccgc gactggcacc gtgaaattca gcaagccatt gaaaccgtag
       61 ctactatcag cggagctcaa ctgattgatt tctttgagcc gatgtatccc taccatcagc
      121 tgcttcacga tgccgtgcac cccaacgcag aaggagcccg catactggct caaacggttt
      181 actcatccat cacaggtgat ttcggcggac tgaaaatgcc cgcgatttac accgataaca
      241 tggtgctgca gcgtaaccgt ccgctcagaa taacgggaac tgccaatgcc ggtgagaaaa
      301 ttacactgac tatcggcaaa caaaaacaca aaaccacgac tgctgataat ggcaaatgga
      361 cagtaacaat caatccattg actgccggaa aaccctatac acttactgta acatctccgc
      421 aaaataaact tacttttcaa aacgttctgg ccggcgaagt ttggctgtgt tccgggcaat
      481 ccaacatgga atttcggctt tcccggagtt ttgaaagcaa aaaagacatc cagtcagccg
      541 ccaatcccga aatcaggctg ttcgacatga aggctcgctg gcttaccaac gactatacgt
      601 ggagtcctgc ggcgcttgat tctgtgaacc gccttcaata tttcactcag ccacggtggg
      661 aagaatgcac accggagaat gctgctgcat tttcggcggt ggcttattat ttcggaaaaa
      721 tgctgcagga cagcctgcgg gttcccgttg ggttgatttg caatgctgtg ggcggatcgc
      781 ccgccgaatc gtgggtggac cgtcgcgagc tggaaaacca tttcccggct attttccgca
      841 actggaccga aaacgacttt atccaggatt gggtacgcac tcgcgcaaaa agcaacatgg
      901 gcaaagacgc cccgaaactt cagcgccatc cgtacgaacc cggctacctc ttcgatgccg
      961 gaatccgtcc gctggcacaa tatcccattg ccggagccat ctggtatcag ggcgaatcca
     1021 acgcacacaa caaagatgcc cacgcaaaac tcttcaaatt actgatagag agctggcggg
     1081 aaaactggga aaatgattca ttgccgttct actttgtgca gctatcgagc atcaaccgtc
     1141 cgtcgtggac ctggttccgc gacagtcagc ggcggctgca ggctgaaatt ccattcacgg
     1201 gcatggctgt gtcaagcgat ttgggtgatt cactggacgt gcatccgata cataagaaag
     1261 aaatcggtga acgactggcc cgaatagcat tgtataacac atacggtaaa acggcggttg
     1321 taccatctgg gccgtttttc agggaagtga atttcaaaag aaaagaagcg attgtcacct
     1381 tcgattatgc cgaagggctc caaacatccg acggaaaatc acctcagaca ttcgaaattg
     1441 ccgaatttcc cggatttttt ttcccggcaa aggcaaaaat taccggtaat caaatacagc
     1501 tgacggctcc ggaagtcaag aatccgaaat tcgtccgata cggatggcag cctttcacgc
     1561 gtgccaacct gataaatgca gcggaattgc cggcatccac atttactacc gaagaataat
     1621 ccgactgcaa attttactga gaaaaaaaat aacaataaaa caaactgaaa atcaaactaa
     1681 ttataaacaa caatgaaaaa cagtaagtat tatccctgga tagtcgtagg actattgtgg
     1741 atagttgcat tgctcaacta cctggaccgg cagatgctct ccactatgca gaagtcaatg
     1801 atgattgaca taacagaact ggcaattgct gaaaatttcg gacgcatcat gggtattttt
     1861 ctgctcatat acggactgat gagccctgtg gcaggcgtca tagccgaccg cattaaccgt
     1921 aaatggctca tagtagggag cttacttgta tggtcggcag taacctacgg catgggatat
     1981 gctaaaacct atgaacaggt ttactggctg cgtgcattga tgggcgtgag cgaagcgctt
     2041 tatttaccca ccggccttgc catgattgcc gatttccatt catcgaaaac ccggtcgctt
     2101 gctatcggca ttcacatgtc gggactttat acagggcagg ctctgggtgg attcggtgct
     2161 acaattgccg ctaatttcag ctggcaggca gtatttcatc tgtttggaat catcggaata
     2221 atttacgccg tggtactcat ttttctttta cacgacaagg aaggtcatgc cggtaccaaa
     2281 accgccaatc ttcagcctgc tgccttacgt tccaccaaag aaccgtttat gaaatccttc
     2341 ggtgtaattt ttggaatgct atcgttctgg attttgctgt tttatttcat ggctccgagt
     2401 ttccccggat gggctacca
//
LOCUS       DAORKP010000232         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15225_length_2402_cov_10.101406, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000232 DAORKP010000000
VERSION     DAORKP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15225_length_2402_cov_10.101406"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>2402
                     /locus_tag="PLN75_09350"
     CDS             19..>2402
                     /locus_tag="PLN75_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRG04361.1"
                     /translation="MKQLTSFKEHPVRFLSVANNGTLCFGFDGEIFMLKQGSTSPQKL
                     KIDISTDVNDEQVKKLNFTTGATSTSNSPDGKQVAFTVRGDVFVTATDFITTKQITQT
                     VAQEADVDFGKDNRSLVYSSYRDGYWNAYIAKISRKEDPNFPNATLIEEEELIKGNKS
                     EKQQPKFSPDGKEVAFMMDRKKLMVYNIADKKLRQITDGSFQHESSGRMTFEWSPDGK
                     WFAIEYVSNAHAPYTNIGIVSAKDGGKVYSVTNSGYTNSNPRWVMDGNAVLFNSEMYG
                     MRNHASWGSMEDVMIAFVNREAYNKFRMNKEEFELFTDAEKEAKKAEEEAKKKEDEKK
                     KDKEKATDKKDEKTKDIVMEFENIEERIVRLTPNSSRLGDAIINKDATKLYYLSAFED
                     DYDMWVYDLREKSTKLLSKLKGGSSSLSMDKEQKTLFVMSGRRMQKMELSNDKFTNID
                     YKADMKLDLAKEREFMFDQVYREEKERFYQVNMHGVDWEKLTKHYRKFLPHINNNYDF
                     SEMLSELLGELNVSHTGSGYRAPASGDRTAELGLFTSYADGKKGLKVDEILVNSPFDN
                     FKSKLKVGDIIEKIDGQEITADTDFYASLEGKAAINTLVSIYSPASGQRWDEVIKPVT
                     SAEVDNLLYNRWVKQRAEDVERLSNGRLGYVHIASMGDDSFRKMYADALGKYYQKDGI
                     VIDIRYNGGGRLHEDIEVFFSSKKYLQQEVRGKDYSEMPSRRWNKASIMLMTEADYSN
                     AHGTPWVYKTMKLGKLVGMPVPGTMTSVNWVTLQDPTVYFGIPVVGYRTAEGTYL"
BASE COUNT          789 a          501 c          567 g          545 t
ORIGIN      
        1 ttgaccaatc cttcggcgat gaaacaactt accagtttca aagaacatcc ggtccgcttc
       61 ctttccgttg ccaataacgg gacactctgt tttggttttg acggagaaat atttatgctg
      121 aaacaaggca gtacaagtcc gcaaaaattg aaaattgaca tatcaacaga cgttaacgac
      181 gagcaagtga aaaaactcaa tttcacgacg ggtgctacat ccacgagcaa ttcgcccgat
      241 ggaaaacagg tggcattcac ggttcgtggc gatgtttttg taactgcaac tgactttatc
      301 accaccaaac aaattactca gacagtagct caggaagccg atgtggattt cggaaaagac
      361 aaccgttcgc tggtgtattc gagctaccgc gatgggtatt ggaatgctta tattgccaaa
      421 atttcccgca aggaagaccc gaatttcccg aatgcgactt tgattgaaga agaagagttg
      481 atcaagggaa ataaatcgga aaagcaacaa ccaaaatttt cgccggacgg aaaagaagta
      541 gcatttatga tggatcggaa aaaactgatg gtctataaca ttgccgacaa aaagttgcgt
      601 cagattaccg acggaagttt tcagcacgaa tcgagcggac ggatgacatt tgagtggtcg
      661 cccgatggaa aatggtttgc catcgaatac gtatcaaatg ctcatgcccc atacaccaac
      721 atcggcattg taagtgccaa agacggcgga aaagtgtaca gtgtaacaaa cagcggctac
      781 actaacagca atccgcgctg ggtgatggac ggaaatgctg ttttgtttaa ttctgaaatg
      841 tatggaatgc gaaatcacgc ctcctggggc tctatggaag atgtgatgat tgcatttgtt
      901 aaccgggaag cttacaacaa attccgaatg aataaggaag aatttgaact ttttaccgat
      961 gctgaaaaag aagctaagaa agccgaagaa gaagccaaaa agaaagaaga cgagaagaaa
     1021 aaagacaaag aaaaagcaac cgacaaaaag gacgaaaaaa ccaaagatat cgtcatggaa
     1081 tttgagaata ttgaagaacg catcgtacga cttacgccaa attcctcccg cctgggcgac
     1141 gccataatca acaaagacgc cactaaactg tactatttat cggcatttga agatgattac
     1201 gatatgtggg tgtacgatct gcgcgagaaa tccaccaaac ttttgagtaa actcaaaggt
     1261 ggaagttcat cgttaagcat ggacaaagag cagaaaacgc tgtttgtaat gagtggcaga
     1321 cgaatgcaga aaatggaact ttccaacgat aaattcacca acatcgatta caaagccgat
     1381 atgaaactgg atctggcaaa agagcgagaa ttcatgtttg accaggtgta tcgggaagaa
     1441 aaagaacgtt tttatcaggt gaatatgcac ggtgtggatt gggaaaaact gaccaaacat
     1501 taccggaaat tcctgcctca catcaacaac aattacgatt tttcagaaat gctctccgaa
     1561 ttgctgggcg aactgaacgt atcgcacacc ggttcaggtt atcgggcgcc tgcttccggc
     1621 gacagaactg ccgaacttgg gcttttcacc agttacgcgg atggtaaaaa aggcttgaaa
     1681 gtggatgaaa ttttagtgaa tagtccgttt gataatttca aatccaaact gaaagtcggc
     1741 gatattatcg aaaaaattga cggtcaggag attaccgctg ataccgattt ttacgcatcc
     1801 ctggaaggaa aagcggcaat aaatacgcta gtatccattt attcgcctgc aagcggtcag
     1861 cgctgggacg aagtgataaa accggttact tccgcggaag tggataattt actttacaat
     1921 cgctgggtaa aacagcgtgc cgaagatgta gaacgcctgt ccaacggacg tttgggttac
     1981 gtacacatag catctatggg cgacgatagt ttccgcaaaa tgtacgccga tgcactcgga
     2041 aaatattacc agaaagatgg catcgtcatt gacattcgct acaacggcgg cggtcgttta
     2101 cacgaagaca tcgaagtctt tttcagtagt aaaaaatacc tgcagcaaga agttcgcgga
     2161 aaagattaca gcgaaatgcc aagccgccgg tggaacaaag cctccatcat gctgatgacc
     2221 gaagccgatt attccaatgc tcacggaacg ccctgggtgt ataaaacaat gaaactcggc
     2281 aaactggtgg gaatgcccgt accgggaacc atgaccagcg tcaactgggt aactctgcag
     2341 gatccgactg tttatttcgg aattccggta gttggttacc gcacagcaga aggcacttac
     2401 ct
//
LOCUS       DAORKP010000233         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15493_length_2378_cov_8.683168, whole genome shotgun sequence.
ACCESSION   DAORKP010000233 DAORKP010000000
VERSION     DAORKP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15493_length_2378_cov_8.683168"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLN75_09355"
     CDS             complement(<1..263)
                     /locus_tag="PLN75_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HRG04362.1"
                     /translation="MTKRTRIFVIAITGLLIIGMAFFPMIKKSLKKEDKKPVPKGQAG
                     GQRNPLVVQGLVLQYSTLNDVFRTKGILLPDEEVDLSFETSGK"
     gene            547..1866
                     /gene="ffh"
                     /locus_tag="PLN75_09360"
     CDS             547..1866
                     /gene="ffh"
                     /locus_tag="PLN75_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRG04363.1"
                     /translation="MFESLSDRLERSFKILKGEGKITEINVAETLKDVRKALLDADVN
                     YKVAKTFTDTVKEKAIGQNVLNALKPQQLMVKIVHDELAMLMGGSSVDINLKGSPTVI
                     LMSGLQGSGKTTFSGKLANLLKTKRGKRPMLVACDVYRPAAIEQLKVLGSQLNIPVYS
                     DEDNKNPVKIAQDAIRQARNMGNDVVIVDTAGRLAIDELMMNEIAAIKQAINPQEILF
                     VVDSMTGQDAVNTAKEFNERLNFDGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVSLVEKAQEQYDEEEARRISKKIAKNQFDFDDFMS
                     QIQQIKKMGNIKDLASMIPGMGKALKDVDIDDDAFKGIEAIIHSMTPYERSNPASLNA
                     SRKNRIAKGSGTTIVDVNRLLKQFDQMSKMMRQATLKQGLPKFKKRR"
     gene            1938..>2378
                     /locus_tag="PLN75_09365"
     CDS             1938..>2378
                     /locus_tag="PLN75_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRG04364.1"
                     /translation="MSNSNQTSNGNSLFGGLKGALIDGKLISQQIKAEIAEEVKAMIA
                     AGKRAPHLAVIIVGHDGGSETYVANKVKSCEEVGFRSTRIAFDDTVSESELLSKIQEL
                     NQDAALDGFIVQLPLPKHISEQKVIEAIDYRKDVDGFHPVNVGRM"
BASE COUNT          718 a          494 c          531 g          635 t
ORIGIN      
        1 atttttcccg aggtttcgaa actcaaatcc acctcttcat cgggcaataa aatgccttta
       61 gtacgaaata catcgttcaa agtgctgtat tgtaacacca atccttgtac taccagtgga
      121 ttacgttgtc cccctgcctg accttttgga accggttttt tgtcttcctt tttcagactc
      181 tttttaatca tggggaaaaa tgccatacca ataatgagta agccggttat tgctattaca
      241 aaaattctgg ttcgttttgt catagggtgt acgaatattt tgtttgaaat ttcactcaaa
      301 attacctatt taacgttgat ttaacatagt atgttctcag tttttatcat tttttgagaa
      361 attcaaacat atttcctgtc aatacatact ccaaaatcat tcaccggatt atgagttata
      421 caattctgca tttttggaaa tcaattttct gcatgttgtc ggagtgacaa tgctttgtct
      481 tttctgctaa ttttactatc tttgcactcc tgaaataaac cagatttttc aatagaaaac
      541 caaattatgt ttgaatcatt aagtgatagg ctggaacggt ctttcaagat actcaaaggc
      601 gaaggtaaaa tcacggaaat caacgtggcc gaaaccctga aagatgtgcg caaagctctt
      661 ttggatgccg atgttaacta caaggtagca aaaacattta ccgatacggt gaaggaaaaa
      721 gctatcggac agaatgtact caatgcgctg aaaccgcagc agctgatggt gaaaatcgtt
      781 cacgatgaac tggcgatgct gatgggcgga agcagtgtgg atatcaacct gaaagggtcg
      841 ccgaccgtga ttctgatgtc cgggttgcag ggttcgggta aaaccacctt ctccggtaaa
      901 cttgccaact tattgaaaac caagcgtggt aaacgtccca tgctcgtagc ctgcgacgtg
      961 taccgtccgg cagccatcga acagctgaaa gtgcttggtt cacagctcaa tatcccggtt
     1021 tattctgacg aagataacaa aaatcctgta aaaatagctc aggatgccat tcgccaggca
     1081 cgcaatatgg gaaatgacgt ggtcatcgtc gatacggccg gtcgtctggc tattgacgaa
     1141 ctgatgatga acgaaattgc ggccatcaaa caggccatca atccgcagga gattctgttt
     1201 gttgtcgatt ccatgaccgg acaggacgca gttaatactg ctaaagaatt caatgaaaga
     1261 ctgaatttcg acggtgtagt tcttaccaaa ctcgatggcg atacacgtgg tggtgcggca
     1321 ctttccatcc gctcagtggt cgataaaccg atcaaattcg tgggtacggg cgagaaaatg
     1381 gatgcactcg atgtgttcca ccccgaacgt atggccgacc gtattctcgg gatgggtgat
     1441 atcgtatcgc tggtggaaaa agctcaggaa cagtacgacg aagaagaagc acgccgtatt
     1501 agcaagaaaa tcgctaaaaa tcagttcgat tttgatgatt tcatgtcgca aattcagcaa
     1561 atcaagaaaa tgggtaacat caaagatctg gcttccatga ttcccggaat gggtaaagcg
     1621 ctgaaagatg tggatatcga cgacgatgca ttcaagggaa tagaagccat cattcactcc
     1681 atgactccct acgaacgcag caatcctgca tctctcaatg caagccgcaa aaaccgtatt
     1741 gccaaaggaa gcggaaccac cattgttgat gttaaccggc tgctcaaaca gtttgatcag
     1801 atgagcaaaa tgatgcgtca ggcaacattg aagcaaggtt tgcctaaatt caagaagaga
     1861 agataacccg tccaaacctt cccgaaggga aggcttagtt tgtaatttgc agaagtttac
     1921 gataaaatca attttatatg tcaaatagta atcaaacatc aaacggaaat tctctcttcg
     1981 ggggattaaa gggggcttta atcgacggaa aactcatctc acaacaaata aaagccgaaa
     2041 ttgccgaaga agtgaaagcg atgattgcag ccggaaaacg ggctccgcac ctggcagtta
     2101 tcattgttgg ccacgacggc ggcagcgaaa cttacgtggc aaacaaggtg aaatcttgtg
     2161 aagaagtggg atttcgttct accagaatcg ctttcgatga tactgtttca gaatctgagt
     2221 tgctctccaa aattcaagaa ttgaatcagg atgcagcgtt ggatggtttc attgttcagc
     2281 ttccgcttcc gaaacatatt tcggagcaaa aagtaattga agccattgat taccgcaagg
     2341 atgtggatgg atttcaccct gtaaatgttg gcagaatg
//
LOCUS       DAORKP010000234         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15587_length_2370_cov_10.002592, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000234 DAORKP010000000
VERSION     DAORKP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15587_length_2370_cov_10.002592"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLN75_09370"
     CDS             complement(<1..279)
                     /locus_tag="PLN75_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRG04365.1"
                     /translation="MATINPKFGKDLKKYGADSFNACYNCGNCTAVCSLSTEDTSFPR
                     ELVRLSVLGLEDELKTSTKPWECYYCGECSTHCPQEAHPGELMMSLRRW"
     gene            complement(279..614)
                     /locus_tag="PLN75_09375"
     CDS             complement(279..614)
                     /locus_tag="PLN75_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRG04366.1"
                     /translation="MKEDNIEEKTILSYYTAEGVPVEKGKTAKYLREKQGIPERVKEN
                     LKEYNQIKRKITEVLKEGDLTVAQIAEKTGLSRPDALYYLMTLVKYGSVKVGEIDDMD
                     EYFTYKLNK"
     gene            complement(668..>2370)
                     /locus_tag="PLN75_09380"
     CDS             complement(668..>2370)
                     /locus_tag="PLN75_09380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRG04367.1"
                     /translation="MEKRIGVYICHCGGNISDYVDVEKVRQSVENDDNIVLAKTTMFA
                     CSDANQKDMVDDINNFNLDGVIVASCSPKLHLVTFRNVTERGNLNKYTYVHSNIREQA
                     SWAHSDNKEGATEKAIHLVKSAIAKARHTQPLDPTKIKAEKSVAVIGAGIAGLKAALS
                     LSEKETHVFVIEKEAAVGGWVAKWKDLFLTDETGDAIIKRLYEELKKRQNVTIFLNSE
                     VVGNTGSIGNISLKIKQKEEEFDITVGSVLVATGFEPYKPKEDEFGFASVPNVITLPE
                     FKQLIDHEKNTLTYNGKPVHNIAYIYCVGSRQPEGENTYCSRYCCTATIHAANQVRNK
                     FRDIQNYHFTRGVRSYGKQELLYAESLQGGDIYLQSYEDGLPTVFARNNKPVVRVNDI
                     LTGGKELEVEADLVVLVTGMAPRSDNKVGSLFKLPKGRDKFFNEIHMKLRPVETVIDG
                     VTIAGACQGPKTIIETVNSALSAATKAYSNISKGVLETDPQIAKIEAPACIGCSDCLP
                     ACPFDAISMARQGGKSIAVINNSLCKGCGMCTPVCPTDAIDLIAYSNVEIMGMIDALV
                     "
BASE COUNT          501 a          585 c          538 g          746 t
ORIGIN      
        1 ccagcggcgc aacgacatca tcagttcgcc gggatgggcc tcctgcgggc agtgtgtgct
       61 gcattcgccg caataataac attcccaggg tttcgtggag gttttcagtt cgtcttccag
      121 tcccagcaca ctcaagcgca ccagttcgcg cggaaaagag gtgtcttcgg ttgaaagtga
      181 acataccgcg gtgcagtttc cacagttgta gcaggcattg aagctgtctg ctccgtattt
      241 tttcagatct ttcccgaatt ttggatttat cgttgccatt atttattcag tttataagtg
      301 aaatactcat ccatgtcgtc gatttcgccc actttcacag agccgtattt gacaagtgtc
      361 atcaggtagt aaagcgcgtc aggtctcgat aagcctgttt tttcagcaat ctgcgctacg
      421 gtgaggtcac cttctttgag cacttctgtg attttacgtt ttatctggtt atactctttc
      481 agattttcct taactctttc cggaatgccc tgtttttcac gcaggtattt ggctgttttt
      541 cctttttcaa ccggtacacc ttcggcagtg taatagctga gaatggtttt ttcttctatg
      601 ttgtcttctt tcataatgat ttcctttgtt ttcagaatta ttgttttctt attcctcaca
      661 aaactgccta aaccagcgca tcgatcattc ccatgatttc cacgttggag tacgctatta
      721 gatcaatggc gtcggtagga cacaccggtg tacacatgcc gcacccttta cagagtgaat
      781 tattgatgac ggcaattgat tttccgcctt gtctcgccat cgaaatggca tcaaacggac
      841 aggctggcag acaatcgctg caaccgatac aagccggtgc ttcgattttg gctatttgcg
      901 gatcggtttc gagcactccc ttactgatgt ttgaatacgc cttggtagct gccgagagtg
      961 ccgaattcac cgtttcgata atggttttcg gtccctggca ggctcctgcg atggtcacac
     1021 cgtcaatcac ggtttccacc ggacggagtt tcatgtgtat ttcgttgaag aatttatcgc
     1081 gtcctttcgg cagtttgaac aaggaaccca ccttgttgtc gctgcggggg gccattccgg
     1141 taaccaatac aaccaggtct gcttccactt cgagttcttt ccctccggtg agaatgtcgt
     1201 ttacgcgcac taccggtttg ttgttgcggg caaaaacggt tggcagtcca tcttcatacg
     1261 actgcaaata gatatcgcct ccctgcaagc tttcggcata gagcaactcc tgttttccat
     1321 acgagcgaac gcctcgtgtg aagtgataat tctgaatatc cctgaactta ttgcgtacct
     1381 gattggcagc gtgaatggtt gccgtacagc agtaacgtga gcagtaggtg ttttcccctt
     1441 cgggctgacg gcttcccaca caatatatat aggcgatatt atgaacgggt tttccgttgt
     1501 aggtaagtgt gtttttttcg tggtcaatca gctgcttgaa ctccggcagt gtaatcacgt
     1561 tgggcacact ggcaaagcca aattcatctt ctttcggttt gtagggttca aatccggttg
     1621 ccacgagtac agagcccact gtaatgtcga attcctcttc cttttgtttt attttgaggg
     1681 aaatattacc gatgcttcct gtattgccaa ccacttcgga attgaggaag atagtcacat
     1741 tctgccgttt ttttagttct tcatacagtc gtttgatgat ggcatcaccc gtttcgtcgg
     1801 tcagaaacaa atctttccac ttagccaccc agccgccgac ggctgcttcc ttttcaataa
     1861 cgaacacatg agtttccttc tccgaaagcg acaatgcggc tttcagtccg gcgataccgg
     1921 ctccgatcac tgccacgctt ttttcggcct taattttggt cgggtcgagc ggctgtgtgt
     1981 gccgcgcttt ggctattgcc gacttcacca ggtgaattgc tttttcggtg gctccttcct
     2041 tattgtcgga atgcgcccac gatgcctgtt cgcggatatt ggaatgcaca tacgtgtact
     2101 tgttcaggtt gccgcgttcg gttacattgc ggaatgtgac caggtgcagt ttcggtgaac
     2161 aggatgccac gattactcca tcgagattga agttattgat atcatccacc atgtctttct
     2221 gattggcatc cgagcaggca aacatggttg tttttgccag cacgatatta tcgtcatttt
     2281 ccacggactg acgcactttc tctacatcca cgtagtctga aatgtttcca ccgcaatgac
     2341 aaatgtatac tcctattctt ttttccataa
//
LOCUS       DAORKP010000235         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15769_length_2353_cov_8.490862, whole genome shotgun sequence.
ACCESSION   DAORKP010000235 DAORKP010000000
VERSION     DAORKP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15769_length_2353_cov_8.490862"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLN75_09385"
     CDS             <1..540
                     /locus_tag="PLN75_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04368.1"
                     /translation="MQTNLFSFFAPTYKIIALAGTLLFTGLLPALPIYMMMKRGQIKD
                     LFISKREERTMPYLFSMLSYLFWLFFLWRTLQFPPEFALVAAGSVLSVFLMVFINLKW
                     KISAHLAGMGGFVGGVFAYSYLMAVNPIGLIVASVIISGLVAVSRVYLKAHTLSQTLA
                     GFCLGFLCVFLPGVIYQLI"
     gene            554..1102
                     /locus_tag="PLN75_09390"
     CDS             554..1102
                     /locus_tag="PLN75_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRG04369.1"
                     /translation="MNHQIKQIAARLRGLRDALELSTEEFALKCEIPVQDYELWESGE
                     SDIPMSFIFHVAQTFGIDTTELLSGETAHANAFFVTRKGKGISVERTKAYKYQALGAG
                     FSGNRAELFEVTVEPNDEPMTLNTHTGQEFNFVLQGTMQLHVAGNDLVLNPGDSIYFD
                     ASKSHGMKALNNEKVVFIAMIV"
     gene            1134..>2353
                     /locus_tag="PLN75_09395"
     CDS             1134..>2353
                     /locus_tag="PLN75_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG04370.1"
                     /translation="MLEKYLKQTEFTGFEDFKANYKLIVPEQFNFGYDVVDAWAAREP
                     NKKALLWTNDKGDCREFTFAEIKKYTDQTASYFQTLGLKKGDIVMAILKRRYEFWFTI
                     IALHKLGITIIPATHLLTKKDIVYRNNAADITTIIAVGEEPIASAISEALAESPSIKH
                     LISVGPIIPEGWEDFHRGIENAPEFTPIPKVNSNDDSMIISFTSGTTGNPKMVTLDFV
                     YPLAHIITAKYWHNLDESSLHLTIADTGWLKAVWGKLYGQWIVGACVFVYDHDKFAPA
                     DMLQMIEKYKVTSLCAPPTIFRFLIREDLTKYDISSLKYCTIAGEALNPEVYKQFYNL
                     TGIKLREAYGQSETTVSVLTPPWIEPKPGSMGLPSPHYDVDLLAPDGSPAEVGEQGQI
                     VIRTDKGYPVGLFKG"
BASE COUNT          659 a          548 c          526 g          620 t
ORIGIN      
        1 atgcagacca atctgttcag tttttttgct cccacatata aaatcatagc tttggccggt
       61 acacttctct tcaccggatt gctgcctgct ttacccattt atatgatgat gaaacgtggt
      121 caaatcaagg atttgtttat ttccaaacgc gaagaacgaa ccatgcccta tcttttttcg
      181 atgctttcat atttgttttg gctgtttttt ctgtggagaa cgctgcagtt ccctcccgaa
      241 tttgcgctgg tggcagccgg gtcggtattg tcggtttttc tcatggtatt catcaatctg
      301 aaatggaaaa tcagtgctca cctggccgga atgggtggtt tcgtaggcgg agtttttgct
      361 tattcatacc ttatggcggt caatcccatc gggctgatcg tagcttctgt tatcatttca
      421 ggacttgtgg ccgtttccag agtttacctc aaagcgcaca ccctttcgca gacactggcc
      481 ggtttttgtc tgggattttt atgcgttttc ttacccggtg taatttatca acttatataa
      541 agatcaagag attatgaatc atcagatcaa acaaatagct gcacgattgc ggggattgcg
      601 cgatgcgctt gaactcagca ccgaagagtt cgccctaaag tgtgaaatac ccgtacagga
      661 ttacgaacta tgggaaagtg gagaatcgga catccccatg agttttattt tccatgttgc
      721 ccagactttt ggaatcgata ccaccgaact cttgtcgggc gaaacagccc atgccaatgc
      781 tttttttgtg acccgaaagg gaaaagggat aagtgtggaa cgcactaaag catacaaata
      841 ccaggcattg ggagccggat tctcgggcaa cagggctgaa ctttttgaag tcaccgtaga
      901 gccaaacgat gaaccaatga ccctcaatac acataccggt caggaattta actttgtgtt
      961 gcagggtacc atgcagctcc atgtggcggg caacgatctg gtgcttaatc cgggcgacag
     1021 tatctatttt gatgcatcca aatcgcacgg catgaaagca ctcaacaatg aaaaggttgt
     1081 ttttatcgcc atgattgtct gaattaccta tactaacata aggcactaaa actatgcttg
     1141 aaaagtattt aaaacaaaca gaattcaccg gctttgaaga tttcaaagca aactataaac
     1201 tgattgtgcc ggaacaattc aacttcggat acgatgtggt ggatgcgtgg gcagcccggg
     1261 aaccaaacaa aaaagcactg ctttggacca atgacaaggg cgattgtcgt gaatttacat
     1321 ttgccgaaat aaaaaaatat accgaccaaa cggcatccta ttttcagacc ctgggattga
     1381 aaaaaggaga tattgtcatg gccattctca aacgccggta cgaattctgg tttaccatta
     1441 tagcacttca caaactgggc attaccatta ttccggcaac gcacttactg accaaaaaag
     1501 atattgttta tcggaataat gcagcagata ttaccaccat cattgctgtg ggtgaggaac
     1561 ccattgcttc ggctatcagc gaagcgcttg ccgaatctcc atcaatcaaa cacctgatat
     1621 ccgtaggacc gatcattccc gaagggtggg aagattttca ccggggaata gagaatgctc
     1681 ccgaatttac gccaatcccg aaagtgaaca gcaacgacga ttcgatgatt atcagtttta
     1741 cctccggtac aaccggaaat ccgaaaatgg taacgctcga ctttgtttat ccgctggcac
     1801 atatcattac cgctaaatac tggcacaacc tcgacgagag cagccttcat ctcaccattg
     1861 ccgataccgg ctggctcaaa gccgtatggg gaaaactata cggacagtgg atagtcgggg
     1921 cttgtgtgtt tgtatatgat catgataaat ttgctcctgc agacatgctt caaatgattg
     1981 aaaaatacaa agttacatcg ctctgtgcac ctccgaccat attccgcttt ctgatacgcg
     2041 aagacctgac aaaatacgac atttcttcgt tgaaatactg caccattgcc ggtgaagcac
     2101 tcaatccgga ggtgtacaaa cagttctata atcttaccgg catcaaactg cgggaagctt
     2161 acggacagag cgaaacaacc gtatcggtgc tcactccgcc ctggatagag ccaaaacccg
     2221 gttcgatggg cctgccaagt ccacactacg atgtggatct gcttgctccc gacggcagcc
     2281 cagccgaagt gggtgaacag ggacaaatag taatccgcac cgacaaggga tatcccgtgg
     2341 gattattcaa agg
//
LOCUS       DAORKP010000236         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_15983_length_2338_cov_6.398598, whole genome shotgun sequence.
ACCESSION   DAORKP010000236 DAORKP010000000
VERSION     DAORKP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15983_length_2338_cov_6.398598"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="lpxK"
                     /locus_tag="PLN75_09400"
     CDS             complement(<1..663)
                     /gene="lpxK"
                     /locus_tag="PLN75_09400"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRG04371.1"
                     /translation="MLKPGKNIIFYILSLIYGSLTTIRNCFFDWGILKQHSFDMPVIS
                     IGNLAVGGTGKTPHTEFLIRQLSEKFRTAVLSRGYRRKTTNFVLADTNSTAQSIGDEP
                     FQIFGKFPQVTVAVDEKRVRGIQNLLKIHPDVQVIILDDGFQHRYVKPGLSILLTDYA
                     NLYTDDYMLPYGTLREFKTNSKRADMVVITKCPEETQPDECKSIAAKLALKPHQKLFF
                     STF"
     gene            complement(804..>2338)
                     /gene="sppA"
                     /locus_tag="PLN75_09405"
     CDS             complement(804..>2338)
                     /gene="sppA"
                     /locus_tag="PLN75_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRG04372.1"
                     /translation="NEVKNLGLDEVLANIKKAKTDKNIKGIYLKGGTLMGGYASVKEI
                     RDALLDFKKSGKFLVAYADTYTQKMYYLCSAADKIYLNPQGMIEFQGIAANTMFLKNT
                     LDKLGVEAQVVKVGTFKSAVEPYVNTKMSDANKEQVTAFIGSIWNNLLTEISDGRKIH
                     KDTLNRYADEMMTFAPAEKLLQYRMVDSLTYIDGMENVLKKMMKVDKDDKLETINQSD
                     LARVPASVKMEKEKIAVIYAVGGIDMGDTEGIISKDLVETIRKVGDEKSVKAVVFRVN
                     SGGGSAYGSEQIWHALSELKAKKPVIVSMGDYAASGGYYISCMADSIVAQPNTLTGSI
                     GIFGVIPNIKGLNDKLGLTYDGVKTNKLSDAIDFNRAFTPDERNIMQNYVNRGYEIFV
                     KRCADGRKKTPDQIKAIAEGRVWTGEDAKKIGLVDKIGGLNDAIAMAAKKAKISNYMI
                     REYPEKESFMTRIMKDFNSTIETRIMEGQLGEKYEVLKQIKRLEKFNGIQAVLPFEIT
                     TN"
BASE COUNT          599 a          523 c          446 g          770 t
ORIGIN      
        1 aaaagttgaa aagaacaact tctgatgcgg tttcagagct aattttgcag caatggattt
       61 acattcatca ggttgagttt cttccggaca ttttgtaatc acgaccatat cagcgcgttt
      121 gctgttcgtc ttaaattctc tcaacgtacc gtaaggaagc atataatcat ctgtgtacag
      181 atttgcataa tcagtcagca gtatggataa tcccggcttc acataccggt gttgaaaacc
      241 gtcatcaagt ataatcacct gaacatcagg atgaattttc agcagatttt ggatgccacg
      301 aacccgcttt tcgtccacag ctacagtcac ctgcgggaat ttaccgaaaa tctgaaacgg
      361 ttcatcgcca atactttgcg cagtgctatt tgtatctgca agtacaaagt tggtagtttt
      421 tcgcctgtag ccgcggctca gaaccgccgt tctgaatttt tcgctcaact gtcggataag
      481 aaattctgta tgcggagttt tccccgtgcc acccactgcc agatttccga tggagataac
      541 aggcatatca aacgaatgct gtttcaaaat tccccaatca aagaagcagt tgcggattgt
      601 ggtcaagctg ccataaatca gagaaagaat ataaaaaata atatttttgc cgggctttaa
      661 caatgatttc tttttataca aagatatgaa atgttttgta aaaaggggaa tgaatagggt
      721 tcaatttgaa attcggagtt cagtataaaa aaaagcgttt ccgtaaaccg aaaacgcttt
      781 gggttatttt acttatcaac aaattaattt gtggtgattt caaatggcag aaccgcctgt
      841 atgccgttga atttttccaa tcgtttgatt tgtttcaaca cttcgtattt ttcacctaac
      901 tggccttcca tgatgcgggt ttcgattgta gaattaaagt ctttcattat tcgggtcata
      961 aaactttcct tttcaggata ctctctgatc atataattgg agatttttgc tttttttgct
     1021 gccattgcaa tagcatcgtt caatccaccg attttatcca ccaaaccaat cttttttgca
     1081 tcttcgccgg tccaaacacg tccttcggct atggctttta tttgatccgg agttttctta
     1141 cgaccgtcgg cacagcgttt tacaaatatt tcataacccc ggtttacgta attttgcatg
     1201 atattccgct cgtcgggggt gaatgctctg ttgaagtcaa tagcgtcgct caatttattg
     1261 gttttcacac cgtcgtaagt caatcctaat ttatcgttca gtcctttgat attcggaatt
     1321 acaccgaaaa taccgataga accggttaag gtattgggtt gagccacaat gctgtcggcc
     1381 atgcacgaaa tgtaatatcc tccggatgcg gcatagtcgc ccatcgatac aatgaccggt
     1441 tttttagctt ttagttcgct cagcgcatgc cagatttgtt cagatccata agcactacca
     1501 ccgcccgaat taacccggaa taccaccgct tttaccgatt tttcatctcc aactttccgg
     1561 atggtttcaa ccaggtcttt cgatatgatg ccttctgtgt cgcccatatc aatgcctcca
     1621 acggcgtaaa tgactgcaat tttctctttc tccattttta ctgatgctgg tacgcgggct
     1681 aaatctgatt ggttgatggt ctccaacttg tcatctttat ctactttcat catttttttc
     1741 aatacatttt ccataccatc gatataggtc agcgaatcga ccatgcggta ttggagaagt
     1801 ttttctgcag gcgcgaatgt catcatttcg tcagcatacc gattcaatgt atctttatgt
     1861 atttttcgcc catctgatat ttcggtcagc agattattcc agatagaacc aatgaaggct
     1921 gtgacctgtt ctttgttggc gtcactcatt ttggtattca catacggttc tacggccgat
     1981 ttgaaagtgc ccacttttac cacttgtgct tcaactccaa gtttgtccag cgtgtttttc
     2041 agaaacatgg tgttggctgc aattccctga aattcaatca ttccctgcgg attgaggtag
     2101 attttatcag cggcagagca taaatagtac atcttttgag tataggtatc ggcatacgcc
     2161 accagaaatt ttcccgattt cttaaaatcg aggagcgcat ccctgatttc cttcacagag
     2221 gcatatccgc ccatcagtgt accgcctttc agatagatac cttttatgtt tttatcggtt
     2281 ttagcttttt ttatgtttgc cagcacctcg tccagaccaa ggtttttcac ctcattcc
//
LOCUS       DAORKP010000237         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_16408_length_2305_cov_7.112444, whole genome shotgun sequence.
ACCESSION   DAORKP010000237 DAORKP010000000
VERSION     DAORKP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16408_length_2305_cov_7.112444"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLN75_09410"
     CDS             complement(<1..455)
                     /locus_tag="PLN75_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04373.1"
                     /translation="MAAQNTDSLLNSYRNNRGIYNIDSLLARGKLMMFANPDKSLEML
                     EKTIVVARKQGDLIKLGDSYRAIGSFYSDIKGNVSAATLNFQLADSIYRKNKGKKSSE
                     GIGAIYHCYGVIYQRQSDYLTSIDYYTKALHVFDSIGNETIRPKTLNNLS"
     gene            806..2062
                     /locus_tag="PLN75_09415"
     CDS             806..2062
                     /locus_tag="PLN75_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRG04374.1"
                     /translation="MKKIVFLVCLSIMFGMQANAQKWLEKVGQKAINTAKQKVENRAA
                     KKAGEATDEALDNLEGKGNQKTDTKETVEGTNKKGTSDNQKSEKLTATSNYDFVAGDK
                     VLLYEDFSQDAVGDFPALWTTNKSGEINTLSIAPGNWLNLNSTEGNWWLLKQIEFPKN
                     FIVEFDVVPKKGAPRYAAAVTIYGENTYKEMSNPYECSNGLIVNIAKTDWESHGIKAG
                     NPKVTGSSSINPVEEEKVNHVIIWVQNRRVRIYHKGEKVLDMPTNLYEGSKFTRFGFM
                     LYRGASSASYVSNIKITTASPDTRSKLLTEGKLVTYGIYFDVNKDVVKAESYGTLKSI
                     ADVLKENPDVRVKIIGHTDSDGDNAKNLDLSQRRAKSVMNELVKVFGIDASRLETDGM
                     GETKPVAPNDTPVNKALNRRVEFVKL"
BASE COUNT          744 a          439 c          491 g          631 t
ORIGIN      
        1 gaaagattat tcagggtttt gggccggata gtttcattgc caatactgtc gaacacgtgc
       61 agcgctttgg tgtagtaatc tatggatgtg agataatcgc tttgtcgttg ataaatgaca
      121 ccataacaat gatatatggc tccaattcct tcgctgcttt tttttccttt gttttttctg
      181 taaatgctgt cggccagctg aaaattgagg gtagcggcac ttacattgcc tttgatgtcg
      241 ctgtaaaaac ttccaatagc ccggtagctg tcgcctaatt ttatcagatc gccttgtttt
      301 cgggcaacga cgatggtttt ttccagcatc tcaagcgatt tgtcgggatt tgcaaacatc
      361 ataagtttgc ctcgtgccag cagtgagtcg atattgtaaa ttccccggtt attccgatag
      421 ctattcagca acgaatcggt attttgtgca gccatttttg atggaaaaca aataagaaac
      481 gcaacaaaat agaagattgc taaatttctc atgcacgata atattttttt aatgtcaaac
      541 ccatacgttt atttttttct aacaaatgta cgattttttc ttatgcaaat ataatgtcga
      601 tatttttttt aatatacccc tttttggggg taatatttgt gtaattgaaa atatattttt
      661 acatctgaca tgttgaaatt gaactatata tgttgagatg taaaaaaagg ctttacaggt
      721 aatcagaaac cataaattga aaatttcaca atcaaaatat ttgaaatatt taaaagggat
      781 tttacaatat taaaaaatca aaatcatgaa aaaaattgta ttcttagtgt gtttgagcat
      841 aatgttcggc atgcaggcaa atgcccagaa atggctcgaa aaagtgggcc agaaggcgat
      901 aaatactgcc aaacaaaaag tagaaaaccg ggctgccaaa aaagccggag aagccaccga
      961 tgaagcattg gacaatctgg aaggaaaagg caatcagaaa accgatacga aggaaactgt
     1021 agaaggaaca aacaaaaaag gaacttcgga caatcaaaag agtgaaaaac tgactgcaac
     1081 ttccaattat gattttgtgg cgggcgataa agtgttgttg tacgaagatt tcagtcagga
     1141 tgcagtcggc gattttccgg cattgtggac aaccaataaa tcgggcgaaa taaacacgct
     1201 gagtattgct ccgggcaact ggctgaacct gaattccacc gaaggcaact ggtggttgct
     1261 gaaacagatt gagtttccta aaaatttcat cgtcgaattt gacgtagttc ccaaaaaagg
     1321 ggctccgagg tatgctgctg ccgttactat ttacggagaa aacacctaca aggagatgag
     1381 caacccctat gaatgcagca acggactcat tgtcaatata gccaaaacag attgggaatc
     1441 tcatggaata aaggccggaa atcctaaagt taccgggtct tcctccatca atccggtaga
     1501 agaggaaaag gtcaaccacg tgattatctg ggtccaaaac cggcgtgtgc gaatttatca
     1561 caagggtgaa aaagtgcttg atatgcccac caacctgtac gaaggcagca agtttacccg
     1621 gtttggattt atgctttaca ggggtgcttc gagcgcatca tacgtcagca atattaaaat
     1681 caccaccgca tcgcccgata cccgcagcaa actgctcaca gaaggcaaac tggttactta
     1741 cggcatttat ttcgatgtaa ataaagatgt ggtaaaagcc gaatcgtacg gaacgttgaa
     1801 aagtatcgcc gatgtgctga aagaaaatcc ggacgtgcgg gtaaaaatca ttggtcatac
     1861 cgacagcgac ggcgacaatg ccaaaaacct ggatttatcg cagcgaaggg caaaatcggt
     1921 gatgaacgaa ctggtaaaag tatttggtat agatgcatca cgtctggaaa cagatggaat
     1981 gggtgaaaca aaacccgtgg ctcccaatga tactcctgta aataaagcac taaaccgcag
     2041 ggtggaattt gtgaaactct gataacttag cttgctgacc gacagatttg catcctgaaa
     2101 acgtaaatct gtcggtcgga ttttcatttt gaaaaataat aaatccgaaa atttaccatg
     2161 aaaacaacat tttcgatcat atttcttctt tcaatgttgg ttatttttcc cgtctcggct
     2221 caaaaaactg cccttgaata tctgaatatt gttcccggaa atcccgattc ggtttgtacc
     2281 tgcaccaaag atgtacgcga aaatt
//
LOCUS       DAORKP010000238         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_16490_length_2298_cov_7.626393, whole genome shotgun sequence.
ACCESSION   DAORKP010000238 DAORKP010000000
VERSION     DAORKP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16490_length_2298_cov_7.626393"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1034
                     /locus_tag="PLN75_09420"
     CDS             129..1034
                     /locus_tag="PLN75_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRG04375.1"
                     /translation="MKPTLFILAAGMGSRYGGLKQLDGLGPGGETIMDYSVYDAVRAG
                     FGKIVFVIRKSFEDDFRNIVLKKYEGIIPTEVVFQDITSIPEGFAFNPERSKPWGTNH
                     AVLMGKEVIKEPFAVINADDFYGKESFEIMAEFLRNVTGKTNEYSMLGYHVGNTLSES
                     GTVARGVCGVDESGNLTNVVERTSIIRKDGKIVFTGDDGNDVEIADNTPVSMNFWGLT
                     PDYFDHSEEEFRNFLKEHGNELKSEFFIPLVINNLIVSGKSSCRVLDTPSDWFGVTYA
                     EDRPEVVAKIQKLVDAGVYPSRLFQ"
     gene            1094..2128
                     /gene="galE"
                     /locus_tag="PLN75_09425"
     CDS             1094..2128
                     /gene="galE"
                     /locus_tag="PLN75_09425"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRG04376.1"
                     /translation="MMPKILVTGGTGYIGSHTVVELQQAGYEVVVVDNLSNSNIEVLN
                     GIEKITGIRPAFENIDCTDYVNMDRMFEKYTGIEGIIHFAASKAVGESVQKPLLYYRN
                     NVVSLMNLLQLMPIHKVPNIVFSSSCTVYGQPDILPVTEEAPIKPALSPYGNTKQICE
                     EIIRDTAYSDGFYQATILRYFNPIGAHPSAEIGELPNGVPNNLVPFITQTAMGIRKQL
                     QVFGNDYNTPDGTCIRDYINVVDLAKAHVIAVERMLQKKSKFNVETFNLGTGRGLSVL
                     ELVNTFQKVNGVKVPHRIVDRREGDIEQVWANPSHANNELGWKAVETVEETLRTSWKW
                     ELNLARRAKQ"
BASE COUNT          693 a          452 c          534 g          619 t
ORIGIN      
        1 tagtgtcttt gtggcaaaaa caaggttttc ggagcagact ctttttttta ttccggaagg
       61 tttagttcag aattaagaaa aatgcgtaat tttgtagtca ttatattctt aaacaaaaca
      121 aacaaactat gaaaccaact ttatttattc ttgcagccgg tatgggtagt cggtatggag
      181 gcttgaaaca actcgacgga ttgggtccgg gaggtgaaac aatcatggat tattcggttt
      241 atgatgccgt acgtgccgga ttcggcaaaa tagtttttgt tattcgcaag tcatttgaag
      301 acgattttcg taatatcgtg ttgaaaaaat acgaaggtat tattcctacc gaagttgtat
      361 ttcaggatat aacaagtatt cctgaaggat ttgcattcaa tcctgaacgg agcaaaccct
      421 ggggaaccaa tcacgcagtg ctgatgggaa aagaggtaat aaaagagcca tttgcagtga
      481 ttaacgccga tgatttttac ggaaaagaat cctttgagat aatggctgaa tttttacgca
      541 atgtaaccgg aaagacgaat gaatactcca tgctgggata tcacgtgggc aacaccttgt
      601 cagaaagcgg tacagtagct cgcggcgtgt gcggagtgga tgaaagcggc aatctgacca
      661 acgtggtgga acgtacttcc atcattcgca aggatggtaa aattgtgttt accggtgacg
      721 atggcaatga cgtggaaata gcagataata cacccgtttc catgaatttc tgggggttga
      781 ctcccgatta tttcgatcat tctgaagaag aattcagaaa ttttctgaaa gaacacggca
      841 atgaactgaa atctgaattt ttcattccac tggtgatcaa caacctgatt gtgagtggaa
      901 aatcttcctg ccgcgtactc gatactccat ccgattggtt cggagtaacc tatgccgaag
      961 accgtccgga agtagtggcc aaaattcaga aacttgttga tgccggtgtg tatccttcca
     1021 ggttgtttca atgattgaaa taatcatttg tgaattgtaa attgtaattt tgtaaattat
     1081 aaatcgataa gatattatgc ctaaaatttt agttaccggc ggaaccgggt atattggttc
     1141 tcataccgtg gttgagctgc agcaggcggg ctacgaagtc gtagtggtgg ataatctctc
     1201 caattcaaac atagaagtgc tcaacggaat cgaaaaaatt accggaattc gtccggcatt
     1261 tgagaacatt gactgcaccg actatgtgaa tatggacagg atgtttgaga aatacaccgg
     1321 catcgaagga attattcact ttgcagcaag caaagccgta ggtgaatccg tacagaaacc
     1381 gctgctctat taccggaaca atgtggtttc gctgatgaat ttactacagc ttatgcctat
     1441 tcacaaggtt ccgaacatcg tgttttcgtc ctcatgcact gtttacgggc agccggatat
     1501 tcttcctgtt accgaagagg cgccaattaa gcctgcactt tcaccctacg gaaatacaaa
     1561 acaaatctgc gaagaaataa ttcgcgatac cgcatattcc gacggatttt atcaggctac
     1621 catcctgcgt tatttcaatc cgataggcgc tcatccgagt gccgaaatag gtgaattgcc
     1681 caatggagtg cccaataacc tcgttccgtt tattactcaa acggctatgg gcatccgcaa
     1741 gcaacttcag gttttcggta acgactacaa tacccccgac ggaacctgta tccgcgatta
     1801 catcaatgtg gtggaccttg ccaaagctca tgttatagca gtagagcgga tgttgcagaa
     1861 gaaaagcaaa ttcaatgtgg aaaccttcaa cctcggtaca ggcagaggac tttcagttct
     1921 cgaacttgtg aatacattcc agaaagtgaa cggagtgaaa gtgccgcatc gcattgttga
     1981 ccggcgcgaa ggcgatattg agcaggtttg ggcaaaccct tcgcatgcca acaacgagct
     2041 cggctggaaa gccgtagaaa ctgtagaaga aacgctccgc acgagctgga aatgggaact
     2101 caatctggca cgacgagcta agcaataaga tctgagtggt taattagtga tttgttggca
     2161 acaagtgtaa aaagacagcc agatgagcag acagatacga tattgacaaa attaaaataa
     2221 gaaaggattt agctttttga caggacagtg caaatttgat ggaaatttat tttgtttttt
     2281 tcttcccccc gcaaaaaa
//
LOCUS       DAORKP010000239         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_17187_length_2245_cov_11.254795, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000239 DAORKP010000000
VERSION     DAORKP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17187_length_2245_cov_11.254795"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2118
                     /gene="fusA"
                     /locus_tag="PLN75_09430"
     CDS             <1..2118
                     /gene="fusA"
                     /locus_tag="PLN75_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRG04377.1"
                     /translation="MSKGDLSYNRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTSWNYLGDKYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVAAFCAVGGVEPQSETVWRQADKYNVPRIGYVNKMDRSGADFYEVVRQVKDVLGA
                     TPCPIQIPIGAEEKFRGVVDLIKMKAILWHDETMGAQYDVEEIPADLLDEAKEWREKM
                     LEACAEFDDVVMEKFFEDPDSLTEKEIKAAIRKGTLEMKINPMICGSSFKNKGVQTML
                     DAVCAYLPSPLDTPEIVGKDPRFEDKEVVRHPEVSEPLCALAFKIATDPYVGRLCFFR
                     VYSGKLDAGSYVYNTRSEKKERISRIFQMHSNKQNPVESIHAGDIGAGVGFKDIRTGD
                     TLCDEAHPITLESMDFPDPVIGITVEPKTQKDMDKLGMGLSKLAEEDPTFTVRTDEQT
                     GQTVISGMGELHLEIIIDRLKREFKVECNQGRPQVSYKEAITQQVEIREVYKKQSGGR
                     GKFADIIVKVEPADQDFEGSLQFVDVVKGGNIPKEYIPAVQKGFQTAMKNGVLAGYPM
                     DTLKVTLVDGSFHPVDSDQLSFEICAQIAYKNACAKAKPVLLEPIMKIEVVTPEESMG
                     DVIGDLNKRRGQVEGMDSSRTGARIVKAKVPLSEMFGYVTALRTITSGRATSSMEFSH
                     YAEVSTTLAKQVVEAAGGKAELL"
BASE COUNT          720 a          435 c          518 g          572 t
ORIGIN      
        1 atgtcaaaag gagatttaag ttataataga aatatcggta tcatggctca cattgatgcc
       61 ggtaaaacaa ccacatccga acgtatcctt ttttatacgg gtttaaccca caagattggt
      121 gaagttcacg atggagctgc tacgatggac tggatggagc aggagcagga acggggtatt
      181 actattacct cagccgctac tactacttcc tggaactatc tgggcgataa atataaaatt
      241 aatttaattg acaccccggg acacgttgac ttcacggttg aagttgaacg ttcgttgcgc
      301 attttagatg gtgcagttgc tgcattctgt gcagtaggtg gtgttgaacc gcagtcggaa
      361 accgtgtgga gacaggctga taaatacaat gttccccgca tcggatacgt gaataaaatg
      421 gaccgttcgg gagctgattt ctatgaagta gtacgtcagg ttaaagacgt actgggtgca
      481 accccgtgtc ctattcaaat tccaatcgga gctgaagaaa aattcagagg cgttgtggac
      541 ctgattaaaa tgaaagcaat tctatggcac gacgaaacca tgggtgctca gtacgacgtg
      601 gaagaaattc ctgctgattt actggacgaa gcaaaagagt ggagagaaaa aatgctcgaa
      661 gcttgtgcag aattcgatga tgtggtaatg gaaaaattct tcgaagatcc cgattcgctc
      721 accgaaaaag aaattaaagc tgcaatacgt aaaggaacgc ttgaaatgaa gattaatccg
      781 atgatttgcg gatcttcatt caaaaataag ggagttcaga ccatgcttga cgccgtatgt
      841 gcgtatctgc caagtcccct tgatactcct gaaatagtag gtaaagaccc ccgctttgaa
      901 gataaagaag tagttcgtca tcccgaagtt tctgaacctt tatgtgctct tgcatttaag
      961 attgccaccg atccttatgt aggtcgttta tgctttttca gagtatattc cggtaaactc
     1021 gatgcgggtt catacgtata taatacccgt tctgaaaaga aagaacgtat ttcgcgtata
     1081 ttccagatgc attccaacaa gcagaatcct gttgaaagca ttcatgccgg tgatattgga
     1141 gcaggcgtag gtttcaaaga tattcgtaca ggcgatacac tttgtgacga agctcatccc
     1201 attacactcg aatccatgga ttttccggac ccggtaatcg gaataaccgt agagcccaaa
     1261 actcagaaag acatggacaa actcggcatg ggattatcaa aacttgccga agaagatcct
     1321 acattcacag ttcgtaccga tgagcaaacc ggacagaccg taatcagtgg tatgggtgaa
     1381 cttcacctcg agattattat cgaccgtttg aaacgcgagt tcaaggttga atgtaatcag
     1441 ggacgtcctc aggtttctta caaagaggca attacacaac aggttgaaat tcgtgaagtc
     1501 tataaaaagc aatccggagg acgcggaaaa tttgccgaca tcattgtgaa ggtagagcct
     1561 gctgatcagg attttgaagg atcattgcag tttgtcgacg ttgtgaaagg gggaaatatt
     1621 ccaaaagaat acattcccgc tgttcagaaa ggattccaga ctgccatgaa aaacggtgtt
     1681 ttagccggat atccgatgga tacactgaaa gtaacgctgg ttgacggatc tttccacccg
     1741 gttgactccg atcagctttc atttgaaatc tgtgctcaga ttgcctacaa gaatgcctgc
     1801 gctaaagcaa aacctgtatt gttggaacca atcatgaaaa ttgaagtggt aacacctgaa
     1861 gaaagcatgg gagatgtgat aggcgacctc aacaaacgtc gcggtcaggt agaaggaatg
     1921 gattcaagcc gtaccggagc ccggattgta aaagcaaaag tgccgctatc tgaaatgttt
     1981 ggatatgtaa ctgcgttaag aacaattact tcaggacgag ccacatcatc aatggaattt
     2041 tctcattatg ccgaagtctc tacaacactg gctaaacagg tagttgaagc agccggaggt
     2101 aaagcagaat tattgtaata aatgtatctc aaaagttcgt aacaaaaacg ttatgaactt
     2161 ttgattgaac aaataaaaaa taaaatttca cttagcgaat gactcttaag tgaaaacgaa
     2221 ttacaaacaa ataaaaaaaa aaaaa
//
LOCUS       DAORKP010000240         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_17705_length_2205_cov_6.887442, whole genome shotgun sequence.
ACCESSION   DAORKP010000240 DAORKP010000000
VERSION     DAORKP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17705_length_2205_cov_6.887442"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLN75_09435"
     CDS             <1..627
                     /locus_tag="PLN75_09435"
                     /inference="COORDINATES: protein motif:HMM:NF037079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRG04378.1"
                     /translation="ISGTIEVTQPITGSSFIHNFTTDGKNSTFYTITGNLSTTKGTIT
                     YENLTLTQCLKIESATNISFNSTEEGVLTLVFIESGKKIKINGNSYTSDATGIVTVTL
                     PAGNHTITKADVMNLFYMKLVYSTTSNVENTLFSKIKIYPNPASDMLHIASEAPLKKI
                     EIYTLTGQLVKMTNGNITSLDISRLESGNYLLKLFTGEGVVSGLFVKK"
     gene            719..2098
                     /locus_tag="PLN75_09440"
     CDS             719..2098
                     /locus_tag="PLN75_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRG04379.1"
                     /translation="MKNSSNISRREFLSMSGALAGTVLIDPVSSLYAAEKISADHLLK
                     KTRVALVGTGHRGTGMWGKSVMEEYAGYVQFVGLCDKNPGRLEVGRQLIGTDCPVYTD
                     FEKMMLETKPEVLIVTTDDNTHDYFISRGMELGADIISEKPMATDERKIQTIIDAEKR
                     TGKTCRVTFNYRYSPHRAKMWEILRSGEIGELTSVDFHWYLDTSHGADYFRRWHRLVE
                     KGGSLWVHKASHHFDLLNWWIESDPESVYALGDLEFYGKNGPFRGENCRNCAHTAECK
                     FFFDINKNERLRRLYPENEKYDGYFRDGCVFRNDVNIYDKMAATIKYKNGVQVSYSLT
                     AYSPYEGYRIAFNGTEGRMDAWIQESNPTSDANYDEIVVYKNFGKRKYIQIPQGAGHG
                     GGDKLLKDQIFIPETADPLKQAAGIRDGALACLIGIAARKSIASGKPVSIDGLTTIQP
                     ENQKIYKRV"
BASE COUNT          699 a          478 c          477 g          551 t
ORIGIN      
        1 atttcgggaa caatcgaagt tactcagcca atcacaggct cctcgtttat tcacaatttt
       61 accaccgacg gcaaaaacag cactttttat acaataactg gtaatctgtc gacaaccaaa
      121 ggaacgatta cctacgaaaa tctgacactt acacaatgcc tgaagattga aagtgctacc
      181 aatatctcat ttaattctac cgaagaaggc gtcctgactc ttgtatttat tgaatccggg
      241 aaaaaaataa aaatcaacgg caactcatac acttctgatg ctacgggcat cgtcacggtt
      301 acgctgcctg ccggaaatca taccattacc aaggccgacg tgatgaacct tttctacatg
      361 aagctggtat atagtactac ttcgaacgta gagaacacat tattttcaaa aataaaaata
      421 tatccgaatc ccgcttctga tatgctgcat attgcatccg aagctccact gaagaaaatt
      481 gaaatctata ccctgaccgg ccaactggta aaaatgacga atggaaatat cacaagtttg
      541 gatataagcc ggttggaaag tggaaattac cttctcaaac tttttacagg tgagggagtg
      601 gtttcagggt tgtttgttaa aaaataacgg acgtgtttac gactattcaa gtcaaaatgc
      661 atttagttgt aaatcagata tatatcaaat ttttagcttg aaaaaatctt tttacgtaat
      721 gaaaaacagc agcaatattt ccagaagaga attcctgtcc atgtcgggtg cattggcggg
      781 tacagtgctc attgatccgg tatcatcact ttacgcagcc gaaaaaataa gcgctgatca
      841 tttactgaaa aaaacaagag tggcgcttgt agggaccggc cacagaggta ccggcatgtg
      901 gggcaaaagt gtgatggagg agtatgccgg atacgtacag tttgtaggtt tgtgcgataa
      961 aaatcccggc aggctcgaag tcggcaggca gctcatcggc accgattgcc ctgtttacac
     1021 cgattttgaa aaaatgatgc tcgaaaccaa acctgaggtg ctcatagtaa caaccgacga
     1081 caatacgcac gattatttta taagccgcgg aatggaactg ggagccgata tcatcagcga
     1141 aaaacccatg gctaccgacg aacggaaaat tcaaaccatc atcgatgccg aaaaaagaac
     1201 cggaaaaacc tgccgcgtga ccttcaacta ccgttactcg cctcaccgcg ccaaaatgtg
     1261 ggaaattctt cgctccggtg aaattggcga attgacatcc gttgattttc actggtacct
     1321 ggatacttcc cacggtgccg attatttcag aaggtggcac cgcctcgtcg aaaagggagg
     1381 ttcactttgg gtgcataaag ccagtcatca tttcgacctg ctcaactggt ggatcgaaag
     1441 tgatccggaa agcgtatatg cgctcggtga cctggaattt tatggaaaaa acggaccgtt
     1501 tcggggtgaa aattgccgaa attgtgcgca cactgcagaa tgtaaattct tttttgatat
     1561 caacaaaaac gaaagactcc gcagattata cccggaaaat gaaaagtacg acggatattt
     1621 tcgggatggc tgcgtattcc gaaacgatgt aaatatttac gacaaaatgg cagctaccat
     1681 caaatataag aacggagtac aggtttcgta ttcgctcaca gcctattcgc cctacgaagg
     1741 ttaccgcatt gctttcaatg gaaccgaagg cagaatggat gcctggatac aggaatcgaa
     1801 tcctacgtcg gatgccaact acgacgaaat agtggtttat aaaaatttcg gaaaacgaaa
     1861 atacatccag attccgcagg gagctggtca cggaggaggc gataaactac ttaaagatca
     1921 gatttttata cccgaaacag ccgatccgct caaacaggca gccggtatcc gcgacggtgc
     1981 gctggcatgc ctcattggca ttgcagcccg caaaagcatc gcttcgggta aaccggtgtc
     2041 catcgacgga cttaccacca ttcaacccga aaatcagaaa atttataaaa gggtataaat
     2101 caaatagata tgttgaaaat aaaatttgtc atcgtttttt ttcaggtttg tttcgtactt
     2161 agtttacatt cacagtacta catttctaaa gtctggacag ccgat
//
LOCUS       DAORKP010000241         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_17730_length_2203_cov_8.669460, whole genome shotgun sequence.
ACCESSION   DAORKP010000241 DAORKP010000000
VERSION     DAORKP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17730_length_2203_cov_8.669460"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2203
                     /locus_tag="PLN75_09445"
     CDS             <1..>2203
                     /locus_tag="PLN75_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRG04380.1"
                     /translation="GGLVRYDGYEFLTLNVNSTPISLKSNYIRKVSEDNFNRLWVISN
                     MGIDVINLLTMQRSSVSGGNESMRNLENISAQSVIKDRDGSIWIMSVEKIHKIDFDKK
                     GNILRIISSENIRGETPHSFTTIHEIDNSIYAGSNGIIYLVSTSQNGKLAFRQLNEAL
                     NFGESTFISSILKKDNTLWIATDNGLYRFEINSSNLIHYVHSESDPNSISQDMVTNLA
                     FFDNGTLVIGTLRGLNFYDPEKNNFTRLMAKSGNKQTISNDFINSLLSDNGVLWIGTE
                     SGGINRMMLPKLITRNYIYNADNSNSISSNPVNAIYEDKNGDMWLGTVEGGLNRKPKG
                     SNSFIHYTTSNTNLSHNSVSSLEMDGNNNLWAGTWGGGINILNINRLSKPSFTQMNFP
                     SQYIAVLKYDSLNNGMWVGTNRDILFYDIEAQKLIRPITEPSFQRIWGTLGCLIDENN
                     NLYMGTSEGLAIVRLNFYNRKTSTFRAVNFTGNGEKIAPLFMRNITSMFLSSDKSIWI
                     GSNGYGICKMMRSKSGYKLQFFDKEHGLAHNTVFGIVEDENGLIWISTGHGLSCYNPS
                     SNHFANYTTNDGLISNQFYWNAFYKSPGNKNIYFGSNEGLTELFGTQQYYTSNTGKIV
                     FTKLQILNKPVWYGNNRYLKNDIAYAEKVKLHEKDKSFSIEFAALGCDNPSTVTYEYR
                     LLGFDEKWINTGADRRFITYTNLRPGKYTFQVRAMSRGNEQMNETAHIQIVV"
BASE COUNT          721 a          473 c          468 g          541 t
ORIGIN      
        1 gaggaggcct ggtacggtat gacggttacg agtttcttac cctgaatgtc aacagtactc
       61 ccatatcgct gaaaagcaac tacatacgaa aggtaagcga agacaatttc aaccgtctct
      121 gggtgatttc gaacatggga attgacgtta tcaacctgct caccatgcaa agatcttctg
      181 tttcgggcgg aaacgagtcg atgcggaatc tcgaaaacat cagtgcccaa agtgtgataa
      241 aagaccgtga tggatccatc tggattatgt ccgttgagaa aattcacaaa attgattttg
      301 acaaaaaagg gaatatactc agaatcattt cttcagaaaa catccggggt gaaacgcccc
      361 attcattcac tactattcat gagattgata acagcatata tgcaggcagc aatggaatca
      421 tctatcttgt ttcgacatcg caaaatggaa aattagcttt caggcaactg aatgaagcgc
      481 tcaattttgg cgaaagtaca tttatttcat ccattctgaa aaaggacaac acactctgga
      541 tagctactga taacggatta tacaggtttg agattaacag tagtaacctg atacactatg
      601 tccacagcga aagtgatccg aattccatct cgcaggatat ggttaccaac cttgcctttt
      661 ttgacaacgg cactttagta atcggtacgc tgcgcggact gaatttttac gatccggaga
      721 agaacaattt cacccgtctg atggcaaaat ccgggaacaa acaaacaatt tcaaacgatt
      781 ttataaacag tttgctttcg gataacggag tgttgtggat aggtacagaa tcgggcggaa
      841 taaacaggat gatgctcccg aaactcatta caagaaatta tatatacaat gcagataatt
      901 caaattccat ttcgtccaac cccgtaaatg ccatctacga ggataaaaac ggagatatgt
      961 ggctcggaac tgtagaaggc ggactgaaca gaaagccgaa aggcagcaac tccttcattc
     1021 attacacaac atcaaatacc aacttaagcc acaattcggt gagttcactt gaaatggatg
     1081 gcaataacaa cctgtgggca ggtacatggg gcggtggaat aaacattctg aacataaaca
     1141 gattgtcaaa accttcgttc actcagatga attttccgtc gcagtacatt gccgttttga
     1201 aatacgactc gttaaacaac gggatgtggg tgggcacgaa ccgggatatt ctcttttacg
     1261 atatcgaggc tcaaaaatta atccgaccga ttaccgaacc ttcatttcaa cgaatctggg
     1321 gcactttggg atgcctcatc gatgaaaaca ataacctgta tatgggcact tcggaggggc
     1381 tggctattgt ccggttaaat ttttataaca gaaaaacatc cacattcagg gcggtgaact
     1441 tcacgggcaa cggagagaaa attgctccgc ttttcatgcg aaacatcacc tccatgtttc
     1501 tgagcagcga caaatcaata tggataggca gcaacgggta cggcatctgc aaaatgatgc
     1561 gctcgaaaag cggatataag ctacagttct tcgacaagga acacggactg gcgcataata
     1621 ccgttttcgg aatcgtggag gatgaaaacg gattaatatg gatcagtaca gggcacggcc
     1681 tctcctgcta caacccgtca agcaaccatt ttgccaacta taccaccaac gatggactta
     1741 tcagcaatca gttttactgg aatgctttct ataaatcacc tggcaataag aatatatatt
     1801 tcggcagcaa cgaaggattg acagaattat tcgggacaca acaatattac acttcaaata
     1861 ccggaaaaat agtattcaca aaacttcaga tactcaataa acctgtgtgg tatggaaaca
     1921 accggtacct caaaaatgat attgcctatg ccgaaaaagt gaaactgcat gaaaaagaca
     1981 aatctttctc catagaattt gcagcactgg gttgcgacaa tccctccaca gtgacgtatg
     2041 aataccgttt gctcggattc gacgaaaaat ggataaacac tggagctgac cgtagattca
     2101 tcacctacac aaacctgcga cccggcaaat acacgtttca ggtgcgggct atgtcgcggg
     2161 gaaacgagca aatgaatgaa actgctcaca ttcagatagt tgt
//
LOCUS       DAORKP010000242         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_17859_length_2193_cov_6.523854, whole genome shotgun sequence.
ACCESSION   DAORKP010000242 DAORKP010000000
VERSION     DAORKP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17859_length_2193_cov_6.523854"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1870)
                     /locus_tag="PLN75_09450"
     CDS             complement(83..1870)
                     /locus_tag="PLN75_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRG04381.1"
                     /translation="MTLRQLFKKLLPFAQPYKNLIFYTLLLTLVGSFAAQINAYVLRF
                     TVDSISSLMVAKKPLSEGFHLLLIISSVLLGKEIVYAVVQFGQKFFGEKLRILIARDL
                     SQKVVERILTYRMAFFTSTENESGKLQTRIDWGISGLTRMVQNFFIDVLPLFANAIVA
                     LIVMFSANFYVGLVGLCIVPIYFFISQKQARKLGGFRRKMRTYRETKNNQIINIIESI
                     NVIKSFVREPVEAEKQRKIQYDMTENQLDTRKTSFMFDSIKSFVEQIGVVLIIILTAY
                     LVLNEQMTIGAIMFHIMLFNNVTAPIRQLHRIYDDMNDALIYSESFFNILEADHEIEK
                     SGAFIPQNLKGKLELRNVSFTYPNGNQALHNVSFTVQPNKITALVGLSGAGKSTVVNL
                     LDKFYEVTEGQILVDGVDIREYDTDNLRQHIGLVLQKNHIFKGSIADNIIYGKIDATM
                     DDVIEAARKAYIHEQVMELPNGYNSEAGQLSGGQQQRIAIARMFIKNPPIIFLDEPTA
                     NLDAIATEQVKKSLDAIKENRTVIIISHSISQIIDAEHIVVLEKGEKAEDGTHDDLLH
                     KKGAYYDIFNAMASSLNFEKIIDSLEEEN"
     gene            complement(1997..>2193)
                     /locus_tag="PLN75_09455"
     CDS             complement(1997..>2193)
                     /locus_tag="PLN75_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HRG04382.1"
                     /translation="LKETFDYYNERFKRGVIPKPPHWGGYRVKPTTIEFWQGRPNRLH
                     DRLLYTLQKDGSWKIERLAP"
BASE COUNT          573 a          481 c          462 g          677 t
ORIGIN      
        1 cgcggataac cggcacacac cgtgtggcgt gtcccagccc ccagttaagc ggacagatta
       61 atattttttt tcgattttcc aattaatttt cttcttcgag cgaatcaatg attttctcaa
      121 aattcaggct gcttgccatg gcattgaaaa tgtcgtagta tgcgcctttt ttatgcagta
      181 ggtcatcatg agtgccgtct tctgcctttt cccctttttc aagcactaca atgtgttcgg
      241 catcaataat ctgggagata ctgtgtgata tgatgataac cgtacggttc tcttttatgg
      301 catccaggct tttttttacc tgttcggtcg cgatggcatc caggttggca gttggttcgt
      361 ccaggaagat aatcggcgga tttttgataa acattcgggc aatggctata cgctgctgct
      421 gcccacccga gagttgaccg gcttccgaat tgtaaccgtt cggcagttcc attacctgct
      481 catggatgta ggcttttcgg gcagcttcaa tcacatcgtc catcgtggca tcaatttttc
      541 cgtagataat attgtcggca atggaacctt taaaaatatg atttttttga agtacaaggc
      601 caatgtgttg cctcaggtta tctgtatcgt attcgcgaat gtccactcca tcgaccagaa
      661 tctgaccttc ggtaacttca taaaatttat ccagcagatt gactaccgtg cttttccccg
      721 caccgctcaa ccctacaagc gctgtaattt tatttggctg aaccgtaaaa ctcacgttgt
      781 gaagtgcctg attaccgttt ggataagtaa aactaacatt ccggagttca agttttcctt
      841 tcagattctg tggaatgaag gcaccgcttt tttcaatctc gtggtcggct tccagtatat
      901 taaagaaact ttcggaataa atcagcgcgt cgttcatgtc gtcgtaaata cggtgcagct
      961 ggcgaatagg agcagtaacg ttgttgaaca gcatgatgtg aaacatgatg gcgccgatgg
     1021 tcatttgttc gttcagtacc aggtatgcag tgaggatgat gatgagcaca acaccgattt
     1081 gctccacaaa actttttatg ctgtcgaaca tgaaacttgt ttttcttgta tcgagctgat
     1141 tttcagtcat atcgtactga atttttcgct gcttttcggc ttcaacaggc tcacgaacaa
     1201 atgatttaat tacgttgata ctttcgataa tgtttattat ctgattgttt ttcgtttcgc
     1261 ggtaggttct catttttcgc cggaagccgc ccagtttccg ggcttgtttc tggctaataa
     1321 aaaagtaaat gggcacaatg cacaaaccca ccaacccaac gtaaaaattg gctgaaaaca
     1381 tcacgatcaa tgccacaatg gcattggcaa acaaaggcag cacatcgata aagaaatttt
     1441 gcaccatccg tgtcaatccg ctgatacccc agtcaatgcg tgtttgaagt tttccgcttt
     1501 cgttttcggt agaagtaaaa aaagccatcc ggtatgtcag aatacgttcc accacttttt
     1561 gtgagaggtc gcgggctatc agtatgcgaa gtttttcgcc gaaaaatttt tggccgaact
     1621 gaactactgc atacacaatt tcttttccga gcaataccga agaaatgatc agcaacaaat
     1681 gaaatccttc actcaacggc ttctttgcaa ccatcagact gctgatgcta tccacggtaa
     1741 agcgcagcac ataggcattg atctgtgcgg caaaagaacc gaccaacgta agtaaaagcg
     1801 tataaaaaat cagattttta tatggctgag caaacggaag tagttttttg aaaagttgac
     1861 gtaaggtcat ttgttattta caatttagtg atttacaatt tacaattggt ttttttggaa
     1921 aatgtaaaca cttgataatc aaattatcat tctgaatggt tggtttcatt tatttaaata
     1981 aacctcaaaa ccattcctac ggcgccaacc gttcaatttt ccagcttccg tctttctgca
     2041 aggtgtaaag cagacggtca tgcagacggt ttggccgtcc ctgccagaat tcgatggtgg
     2101 ttggtttcac ccggtaacct ccccagtgcg gcggtttggg gattacaccc cgtttgaacc
     2161 gttcgttgta gtagtcaaag gtttctttca gcc
//
LOCUS       DAORKP010000243         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_18108_length_2176_cov_10.053277, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000243 DAORKP010000000
VERSION     DAORKP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18108_length_2176_cov_10.053277"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..966)
                     /gene="proC"
                     /locus_tag="PLN75_09460"
     CDS             complement(178..966)
                     /gene="proC"
                     /locus_tag="PLN75_09460"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRG04383.1"
                     /translation="MNRISIIGGGNMGGAIAKGLLKSNQIAAKDITISDISEKTLNNF
                     RVISDEWKLTTSNEEAIADSEIIILAVKPWLVEKIAGEIREKTDYNNQIIVSIAAGVE
                     LKTLQSLFSENAILFRLIPNTAIEVMESVSTVTSINASKEQIEKICLIFEEMGKVFYV
                     ENEKLLNAFMALSSCGIAYAFRYIRAAMEGGVEMGIYPETAKEVIMQTLKGAVKLLEE
                     NHSHPETEIDKVTTPGGITIRGLNEMEANGFTNAVIKGLKKSLP"
     gene            complement(1031..>2176)
                     /locus_tag="PLN75_09465"
     CDS             complement(1031..>2176)
                     /locus_tag="PLN75_09465"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRG04384.1"
                     /translation="GIIDIGQIIDITRLTKIEGVRVGLPFRTNEHLWKNLMLGGQAAY
                     GFRDKKMKYTGEIQWKLPVQSKRIILGAKYLNDYRRIDYDYNNFLWREDPLATGDENF
                     VSTFFDFRSQNRMSKRHEMSAFLFNDWTPDIESKWIFRDVTYFQNELLPMIQNERSFN
                     SLHDRNFSFTTRFSFGEKIIEDHFQRIYLKSEKPVLYATLEGGHFKYGDEKGNYGRLS
                     TTLLHRGQFALGEWRYMIEAGKILGSVPYPLLKFIQGKESGAYNRFEFAMMNNREYIA
                     DTYGTLFTELITNGIIFNNIPLIKHLNLREIASFKMAYGTLSDSHATRMNIPSPSAGF
                     TQPYSEASIGFCNLFGVLSIQYIRRLSDLNKANIQKSGIKLNVMVTF"
BASE COUNT          576 a          468 c          398 g          734 t
ORIGIN      
        1 tcaaagatac ctcctcaacg cgtcctcgcg ttccaacact tcttttagtt ggtctgatgt
       61 aatcataaaa gcccctccaa acctccccaa aagggaggct ttttcgtttg ttaattattg
      121 ttattgttaa atattcaaat tttttctttc ctgccaaatt cccttgcttt ggagggatta
      181 tgggaggctt tttttcaatc ctttaatcac ggcgttggta aaaccattgg cttccatttc
      241 gttcaagccc ctgatggtaa ttccacccgg tgtagttacc ttgtctattt cagtttcggg
      301 atgagaatga ttttcttcta ataatttcac agcacctttc agcgtttgca taatcacttc
      361 tttggcagtt tccgggtaaa tacccatttc cacaccgccc tccatggctg cccgaatata
      421 acggaaagca taggctatac cacaggatga gagagccatg aatgcgttga gcaatttttc
      481 attctcaaca taaaatacct tacccatttc ttcgaatatc aaacagattt tctcaatttg
      541 ttccttgctt gcattaatcg aagtcactgt tgaaacgctt tccattactt caatggccgt
      601 gtttgggatt agcctgaaaa gaattgcatt ttccgaaaaa agagactgaa gggtttttaa
      661 ttcaactccg gctgcaattg aaacgatgat ttggttgttg taatctgttt tttccctgat
      721 ttcaccggct attttttcta caagccaagg tttgactgcc agaataatga tttcagaatc
      781 tgctattgct tcctcattgg atgtagttaa tttccactca tcagaaatca ctctgaagtt
      841 attcagcgtt ttttcagaaa tatcgctaat ggtaatatct ttggcagcta tttggttgct
      901 tttgagtaag ccttttgcaa tggctccacc catattcccg ccaccgataa tgctaattct
      961 attcattgtt gatttttatt ttttgtgcaa aagtactcat ttcattaaaa tattgcaatt
     1021 cttcaatcaa tcagaatgtt accattacat tcagtttaat tccgcttttt tgtatattgg
     1081 ctttgttcag atcagacagt cgcctgatat actggatgga taaaacgcca aaaaggttac
     1141 aaaatccgat acttgcttcg gagtatggtt gcgtaaatcc ggctgaaggt gatggtatgt
     1201 tcatgcgtgt ggcatgcgaa tcgctcagcg tgccgtaagc cattttgaat gaagctattt
     1261 ctctcagatt caaatgtttt atcagcggaa tattgttgaa aattatccca ttcgtgatta
     1321 actcggtaaa aagagttcca taagtgtcgg caatatattc acggttgttc atcatggcaa
     1381 attcaaaccg gttgtaagcg ccgctttctt ttccctgaat gaacttgagc agcggatagg
     1441 ggacgcttcc cagaattttg cctgcttcaa tcatgtatcg ccactctcca agtgcaaact
     1501 gtccgcgatg cagcaaagta gtgcttaaac ggccataatt tcccttttca tcgccatatt
     1561 tgaaatgtcc tccctccagc gtggcataca aaaccggttt ttcggattta agataaatcc
     1621 gctgaaaatg gtcttcaatt atcttctcgc cgaatgaaaa acgggtggta aaagaaaaat
     1681 tccggtcgtg caatgaatta aacgatctct cgttttgtat cattggcaac aattcatttt
     1741 ggaaataggt aacatccctg aaaatccatt tcgattcaat atcgggtgtc cagtcgttga
     1801 aaagaaaagc gctcatttca tgccttttac tcattctgtt ttgagaccgg aaatcaaaga
     1861 atgtggaaac gaagttttca tcgcccgtgg caagcggatc ttcgcgccat aggaagttgt
     1921 tgtagtcgta atctattctt cggtaatcat ttaagtattt tgcacccaat attattcttt
     1981 tgctttgcac cggtaatttc cattgaattt caccggtgta tttcattttc ttatcacgaa
     2041 aaccgtaagc agcttgtcct cccagcatca gattcttcca cagatgttcg ttggttctga
     2101 atggcaatcc aaccctgaca ccctcgattt ttgttaaacg tgtaatgtca attatctggc
     2161 caatatcaat aatccc
//
LOCUS       DAORKP010000244         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_18131_length_2175_cov_6.145755, whole genome shotgun sequence.
ACCESSION   DAORKP010000244 DAORKP010000000
VERSION     DAORKP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18131_length_2175_cov_6.145755"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..>2175)
                     /gene="pnp"
                     /locus_tag="PLN75_09470"
     CDS             complement(192..>2175)
                     /gene="pnp"
                     /locus_tag="PLN75_09470"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRG04385.1"
                     /translation="RFPGGFTRREGRAGDYEILVSRLVDRALRPLFPDDFHAEVYVNV
                     MLISADGVDMPDALAGLAASAALAVSDVPFHGPISEVRIGRVNGEYIVNPTFEQLASA
                     DIDIIVAATLENIMMVEGEMSEVSEDELLTAMKVAHEAIKIQCQAQIELMEAAGKTEK
                     RTYCHEENDEELKKDIWEKTYDKVYAEASSCNTDKHLRAKNFKSIVEEYVAGIDPEVA
                     AAKKELIGRYYHSVEKEAMRRSILDEGKRLDGRKTNEIRPIWSEVDYLPGAHGSAVFT
                     RGETQSLTTVTLGTKMDEKLIDEVLTQGKDRFLLHYNFPPFSTGEARASRGVGRREVG
                     HGNLACRALKSMIPDGYPYVIRIVSDILESNGSSSMATVCAGTLSLMDAGVQMKKPVS
                     GIAMGLISEKNATNYAILSDILGDEDHLGDMDFKVTGTRDGITATQMDIKCDGLSFEI
                     LEKALNQAKEGRLHILDKIQETLAEPRPDFKPNAPRIVVMMIPKELIGAVIGPGGKII
                     QNMQAETGAVISIEEIGDQGRIEIAATNKASIDAAVAKIKGIVAIPEIGETYPSKIKS
                     IMPYGAFVEFMPGKEGLLHISELDWKRIETMDEAGLKEGDMIEVKLLDVDQKTGKFKL
                     SRKALLPRPPREDKPRDERSDDKPREHRGPRPERRD"
BASE COUNT          537 a          504 c          489 g          645 t
ORIGIN      
        1 cctatctttc ggcacgaatt tctacggtga aagaagtcat acattctatg atttttgagt
       61 ttaaaaaacg ggagaccgat ttaatggcag gatcgaaagt ttagtcaaca ccgaataaaa
      121 tgaaaagagg ctgtcccaaa attgaatttg agacagcctc tgatttttta tacgattaca
      181 ttcgttcgtt tttagtcacg tctttcagga cggggacctc tgtgttcgcg gggtttgtca
      241 tctgatcttt cgtcacgtgg tttatcttcg cgtggaggac gaggaagcaa tgctttacgt
      301 gaaagtttga atttaccggt tttctgatcg acatcaagca gtttcacttc aatcatatcg
      361 ccttctttca gaccggcttc gtccatagtc tcaatgcgtt tccaatccag ttcggaaatg
      421 tgaagcaatc cttcttttcc aggcataaat tcaacaaatg caccataagg catgattgat
      481 ttaatcttgg atggatacgt ttctccgatt tcgggaatag ctacgatgcc tttgattttg
      541 gcaacagctg catcaatcga agctttgttg gtggcagcaa tttctattct tccctgatct
      601 ccgatttctt cgatggaaat tacggcaccg gtttcagcct gcatattctg gatgattttt
      661 ccaccagggc ctattacagc gccaatcaat tctttcggta tcatcatcac cacgatgcgg
      721 ggtgcatttg gtttgaagtc gggacgtggt tcagccagcg tttcctggat tttgtccaga
      781 atgtgcagac gaccttcttt ggcctgattc aatgcttttt caagaatttc gaaggagagg
      841 ccatcacatt tgatatccat ttgagtagct gtgataccat cgcgggttcc ggttaccttg
      901 aagtccatat ctcccaggtg atcctcatcg cccagaatgt cggaaagtat ggcgtaattg
      961 gttgcatttt tttcggaaat caatcccatg gcaatacctg ataccggttt tttcatttga
     1021 actccggcat ccatcagcga tagcgtacca gcgcaaacag tagccatgga agatgaaccg
     1081 ttggattcca atatatccga aacaatacgg attacgtacg gataaccgtc gggtatcatc
     1141 gatttcagtg cacgacaggc caggttaccg tgaccaactt cgcggcgacc aacaccacgc
     1201 gatgctcttg cttcgccggt ggagaagggg ggaaaattat agtgtaacag gaaacggtct
     1261 ttgccttgtg tcaacacttc atcaatcagt ttttcatcca tttttgttcc gagagtaacg
     1321 gttgtcagcg actgagtttc accgcgggtg aaaacagctg aaccgtgagc tccgggcaga
     1381 taatccactt ccgaccagat tggacggatt tcattggttt tgcgaccatc cagacgtttg
     1441 ccttcatcca gaattgaacg gcgcatcgct tccttttcta ccgagtggta gtaacggcct
     1501 atcagttctt ttttggctgc tgctacttca gggtcaattc cggctacata ttcttcaact
     1561 attgatttga agtttttggc acgcaggtgt ttgtcggtgt tgcacgaaga ggcttcggca
     1621 tagaccttat cgtaggtttt ttcccaaata tcttttttca gttcttcatc attctcttca
     1681 tgacagtaag ttcttttttc ggttttaccg gcagcttcca tcagttcaat ttgtgcctga
     1741 cactgaattt tgatagcttc gtgagctact ttcatcgcag taagcagttc atcttcgctt
     1801 acttcactca tttcgccttc taccatcatg atgttttcaa gcgttgcagc cacgataata
     1861 tcgatgtctg cactggcaag ctgttcgaaa gtaggattaa cgatatattc gccattcaca
     1921 cgaccgatac gaacttcgga aatcgggcca tggaaaggaa catcggaaac tgcgagggca
     1981 gctgaagcag ccagaccggc taatgcatcg ggcatatcca caccgtcggc tgaaatgagc
     2041 attacattca cataaacttc agcatgaaaa tcatcgggga acaggggacg cagtgcacgg
     2101 tcaacgaggc gtgaaaccag aatttcataa tctccggcac ggccttcacg acgggtaaat
     2161 ccaccgggga aacgt
//
LOCUS       DAORKP010000245         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_18457_length_2154_cov_7.912816, whole genome shotgun sequence.
ACCESSION   DAORKP010000245 DAORKP010000000
VERSION     DAORKP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18457_length_2154_cov_7.912816"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLN75_09475"
     CDS             complement(<1..197)
                     /locus_tag="PLN75_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DjlA"
                     /protein_id="HRG04386.1"
                     /translation="MAGFTKWITGGLGFVLGGPIGGLIGFAIGSLIDGSAQLVNYGEY
                     QEQTRRGTNPYSRTAEGDFKM"
     gene            686..1453
                     /locus_tag="PLN75_09480"
     CDS             686..1453
                     /locus_tag="PLN75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRG04387.1"
                     /translation="MKKTGILLLSLLLIQSLFAQENHRILLGFDFGGGTVAGELSNNW
                     SIRQDLSSYSDYGNFSSGVRNESSIMYFSFKPELIIKQNRLSLLSGLRFTLFDSYLKG
                     GINDEYFFLRYKSASSGTEFARISTLSETVSYLSIPLELKYVPIQISNVGFYAKVGTE
                     AGVKLNSKKSIAFTSETMNEYSEEILNTNPTKINSMYSTIYGAFGVRWESLKGTQVNM
                     EWVLPSQFLTKNNLSQITPEMYSGFQLSVMFPLQKTK"
     gene            1491..2087
                     /locus_tag="PLN75_09485"
     CDS             1491..2087
                     /locus_tag="PLN75_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04388.1"
                     /translation="MKKTILLPVLSILMLIVAGCSNEDYRLNKSVFIEDITNPGLPEY
                     SEWGYNTFGAYIDRVPFVSDMVNLPAKIIVNPDTFNLVLNGKINNSEPASLKFRIFGY
                     APATYSDLVSLDGKSYNLKDNSCLVELTEAGSVTQLKIISGELKFVRAQKLFVDKELV
                     KTILSGTFHIQTFRKNEPITISGGRFDLGFGTENFYNF"
BASE COUNT          691 a          439 c          399 g          625 t
ORIGIN      
        1 ctcattttga aatcgccttc agcagttctg ctgtaaggat ttgtgcccct ccgggtttgc
       61 tcctgatact cgccataatt cacgagttgc gcagagccgt caatcagtga gccgatagca
      121 aagccaatca atccaccaat cggtccgccc agcacaaagc ccagtccgcc cgttatccat
      181 tttgtaaaac cagccattcc taatctattt tttttgaatt gacaaatagc gttcaaatga
      241 gggatttacc atctactttt tacaactaaa attaatctgc aaagatagta aagatatttt
      301 ctttatcctt caaaactctc aaaatagata atatttgaat tattgctgtg ttgaatgagt
      361 cactcgtaaa ttcatttttc ctaacaaaga attaattaac gtaatttaac atacatcgat
      421 tgcagcgttt tgcctgtaaa atccatcata ttagaaagac actttgtatt ttaccctgga
      481 aaaaacgctg cgaatgctta caataatatt actcctaaca atcataagtc tgaaaatcaa
      541 gacaaaaggt ggtatgaatt atcggatttc agaaaaatca acgacgtttt ggttcacagt
      601 gagttcattt accaaaagaa aatttcagca agcaaaacaa aataatatct ttgcaaataa
      661 atcatcagcc caacgtgaac aatttatgaa aaaaacggga atacttttac tgtctctttt
      721 attgattcaa tcgctgtttg cacaggaaaa tcatcggatt ctgctcggat ttgattttgg
      781 aggaggtacg gtggcaggcg aattgagcaa caactggtca atacgtcagg atttaagctc
      841 ctactccgat tacggcaatt tttcatccgg cgtcagaaat gagtcttcga tcatgtattt
      901 cagtttcaaa ccggaactga taatcaaaca aaacaggctg agccttttgt cgggacttcg
      961 atttacactt tttgacagct atctgaaagg tggaattaat gacgagtatt tctttctgcg
     1021 ctataaatca gcttcttcgg gcactgaatt cgcccggatt tccacacttt cggaaacagt
     1081 gagctacctg agcattccgt tggaactgaa atacgtaccc attcagatca gcaacgtagg
     1141 tttctacgca aaagtaggaa cggaagccgg tgttaagttg aattcaaaaa aatcgatagc
     1201 attcacttca gaaaccatga atgaatacag cgaagaaatc ctcaacacaa atccaacgaa
     1261 gataaacagc atgtattcaa caatttacgg agcatttgga gtaagatggg aatcgctgaa
     1321 aggcacacag gtaaacatgg aatgggtgct tccttcgcaa tttctgacta agaacaacct
     1381 tagccagatt acgcctgaaa tgtattcagg atttcagctc tcggtcatgt ttccactgca
     1441 gaaaacgaaa taaaaataaa atttccaccg aaaaaatagt cagataaaat atgaaaaaaa
     1501 caatcctgtt gcctgttctg agtattctga tgctcattgt tgccggatgt tccaacgaag
     1561 attaccgcct gaacaagtcc gttttcatag aagatataac caatccgggt cttccggaat
     1621 actccgaatg gggttacaac acatttggtg cctatattga tagagttccc ttcgtatcgg
     1681 atatggtgaa tttacctgcc aaaattatcg taaatccgga tactttcaat ctcgtgctga
     1741 atggaaaaat caacaattcg gagccggcat cacttaaatt ccggatcttc ggttatgctc
     1801 ctgcaactta ttctgatctg gtttcactcg acggaaaaag ttacaatctt aaggataaca
     1861 gttgcctggt ggagctgaca gaagcgggca gtgtaacgca gctcaaaatc atcagcggag
     1921 aattaaaatt cgtacgggca cagaaacttt ttgttgataa ggaacttgtt aaaacaatcc
     1981 tttcaggaac atttcatatc cagactttca gaaaaaatga accgattact atttcgggcg
     2041 gtcggtttga tttaggattt ggaaccgaaa atttctataa tttttagcac gaactttttt
     2101 attccaacca tcggatatgt acatcctgca gattattttc tgcaggattt tcca
//
LOCUS       DAORKP010000246         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_19472_length_2094_cov_6.127513, whole genome shotgun sequence.
ACCESSION   DAORKP010000246 DAORKP010000000
VERSION     DAORKP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19472_length_2094_cov_6.127513"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..2037)
                     /locus_tag="PLN75_09490"
     CDS             complement(139..2037)
                     /locus_tag="PLN75_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRG04389.1"
                     /translation="MTNYMQVENLTKSFGDNLLFENISFTITDNQRVALIAKNGAGKT
                     TLLNIIADREGYDSGNIYLKRDLRIGYLDQSPDFPKELSVIDACLQTDNDIVRTIAEY
                     EHVMLHPDKDKMNDVLARMEILKAWDYETRIKQILGKLKITDFEQKIGHLSGGQLKRV
                     ALANVLISEPDLLILDEPTNHLDLEMVEWLEEFLKRSTMSLLMVTHDRYFLDRVCTTI
                     LEIDNQALYSYSGNYSYYLEKRQERIDTFNAETESVKNIYRRELEWIRSTPQARSHKS
                     RSRTDRFKDIEERAKQNLITSNVKLGVKSSYLGSKIFEAKNICKGYENLTILDNFNYT
                     FARYEKMGIVGKNGTGKTTFLKMLLGQIKPDSGSFDIGETVKFGYYSQDGLAFDEQMK
                     VLDVVQNIAEEIDLGGGKKLSASQFLQHFLFTPETQHNYVYKLSGGEKRRLYLCTVLM
                     KNPNFLVLDEPTNDLDIVTLNILEDYLQNFKGCVIVVSHDRYFMDKVVDHLLVFRGNA
                     EIKDFPGNYTQYREEETAPQPPKGGVEDEKASKNAKKSAVANSLSSGDNRAEGKPRKM
                     SFKEKQEFEALEKEIPQLEAEKADIELQLSSGSLDNETIIKVSERYAELTDLIDEKTM
                     RWLELSEI"
BASE COUNT          502 a          473 c          470 g          649 t
ORIGIN      
        1 gcgtggctac caattgcgtt tttaaaaccc cctgccccta aaggggagaa aattaataca
       61 catttaatat tcgcatcaaa gtattgtgaa taaagaactt cgctaacttt ccaattgtaa
      121 atttcactaa ctttcctgtt aaatctcgct cagttcgagc caccgcatcg ttttttcatc
      181 tatcagatca gtcagttcgg catagcgttc ggaaactttt attatagttt cattatccag
      241 cgagcccgac gatagttgaa gttcaatatc cgctttttca gcttccagtt gcggtatttc
      301 cttttccagc gcttcaaatt cctgcttttc tttgaaactc attttgcggg gcttaccctc
      361 agcccgatta tcgccggaag aaagggagtt ggcaaccgct gactttttag cattctttga
      421 agctttctcg tcttcaactc cccctttagg gggttggggg gctgtttctt cctccctgta
      481 ctgcgtataa tttcccggaa aatccttaat ctccgcattt ccgcggaaaa caagcagatg
      541 atccaccact ttatccataa aatagcggtc gtggctcacc acaatcacgc agcctttgaa
      601 attctgcagg taatcttcca aaatgttcag tgtaacgata tccagatcat tggtaggttc
      661 atcaagaacc agaaaattcg gatttttcat cagcacggtg caaagataca ggcgccgttt
      721 ttcgccgccg ctgagtttgt acacgtaatt gtgctgcgtt tcgggagtaa aaaggaaatg
      781 ttgcagaaac tgcgaagccg aaagcttttt accgcctccc aaatcaattt cttcggcaat
      841 attctgcact acatccagca ctttcatctg ttcatcgaac gccagcccat cctgactgta
      901 ataaccgaac ttaaccgttt cacctatgtc aaaactgccg ctgtcgggtt ttatctgccc
      961 cagaagcatt tttagaaaag tggtttttcc cgttccgttt ttcccgacga tgcccatttt
     1021 ttcgtagcgg gcaaaagtgt aattgaaatt atccagaatg gtcaggttct cgtaaccttt
     1081 gcagatgttt tttgcttcga aaattttgga accgaggtaa cttgatttta caccgagttt
     1141 tacgttgctc gttatcaggt tttgtttggc ccgttcttca atgtccttaa accggtcggt
     1201 acggctgcgc gatttgtgcg agcgtgcctg cggagtactg cgtatccatt ccagttcgcg
     1261 gcggtagatg ttttttacac tttcggtttc ggcattgaag gtatcaatcc gctcctgccg
     1321 tttttccagg taataactgt aattgccgga ataggagtaa agagcctgat tgtcaatttc
     1381 gagaatggtg gtgcaaacgc gatcgaggaa ataacggtcg tgcgtaacca tgagcagcga
     1441 catggtggag cgtttcagaa attcttccag ccattccacc atttcaaggt cgaggtgatt
     1501 ggtaggttca tcgagaataa gcaggtcggg ttcgctgata aggacattgg ccagcgccac
     1561 ccgtttgagt tgtccgccgg agagatgtcc gattttttgt tcgaaatcgg tgattttcag
     1621 tttaccgaga atttgtttga tgcgggtttc gtaatcccag gctttcagaa tttccatgcg
     1681 tgccagcacg tcgttcattt tatccttgtc gggatgaagc atcacgtgtt cgtattcggc
     1741 aatggtgcgc acgatgtcgt tatcggtttg caggcaggca tcaatgacag aaagttcctt
     1801 cggaaaatcg ggcgactgat ccaggtagcc gatgcgcagg tcacgtttca aatagatatt
     1861 accgctgtcg tacccttcgc ggtcggcaat gatgttgagc agcgtggttt ttcccgcacc
     1921 atttttggca atgagtgcta cccgctggtt gtcggtgatc gtaaaggata tattttcaaa
     1981 aagcagatta tcgccaaacg atttggtcag attttctacc tgcatgtagt tggtcatacg
     2041 aatgtaattt tttacaaaga taaggttttg ggataagata tgaattattt atta
//
LOCUS       DAORKP010000247         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_19866_length_2072_cov_6.678235, whole genome shotgun sequence.
ACCESSION   DAORKP010000247 DAORKP010000000
VERSION     DAORKP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19866_length_2072_cov_6.678235"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1049
                     /locus_tag="PLN75_09495"
     CDS             210..1049
                     /locus_tag="PLN75_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRG04390.1"
                     /translation="MNKIISKAYFSTNVVKFEVEAPLIAKSRKAGHFVMVKVGEKGER
                     IPLTIADADVQKGTITLVVQKMGVSSTKLCALNEGDFITDMVGPLGKATHIENFGTVV
                     CACGGVGTAPMLPIVAALKAAGNRVITIIAARTKDLIILEEQMKVNSDEVIVMTDDGS
                     YGTKGLVTNGVEAVIHREKVDLCVTIGPAIMMKFVSKLTEKYEVPTVASLNTIMVDGT
                     GMCGACRVSVGGKTKFVCVDGPEFDAHLVDFDEMLMRLGGYKDIEREEMEKMQCASGA
                     GKN"
     gene            1297..>2072
                     /locus_tag="PLN75_09500"
     CDS             1297..>2072
                     /locus_tag="PLN75_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRG04391.1"
                     /translation="MNLTEERAQSWRADLRSAIKAKDRTAQPRVHMPELDADYRSRTR
                     VEEVNLGLTPEMAMKEAQRCLDCANPTCMEGCPVSIYIPKFVKQIEIGDFLGAARTLK
                     ETSALPAVCGRVCPQEKQCEAQCIHTKMKKEAVAIGHLERFAADYERESGNISVPEVA
                     APNGLKIAVVGSGPAGLAFAGDMAKLGYDVTVYEALHEIGGVLKYGIPEFRLPNAIVD
                     VEIENLRAMGVKFITNCIIGKTISIDDLKEEGYKGVFVASG"
BASE COUNT          623 a          366 c          536 g          547 t
ORIGIN      
        1 ggtataaaaa gggtgagcgt aagtacatta gaaagacgca aatgtacgaa ataattcatt
       61 aacattcaca aaaataggat aaccgttttt ttacaaaagg gtaatttctt ctttgtttta
      121 ttaataaata agacttttaa aattacaatg tgcaggtggc aagcgatttt tttttaattt
      181 tgcaggtcaa taaatataat tatcctccaa tgaataaaat tatcagcaaa gcgtattttt
      241 cgaccaatgt agtaaaattt gaagtagaag cccctttgat tgcgaagtcg cgcaaagccg
      301 ggcattttgt aatggtaaaa gtaggcgaaa aaggggagcg tattccactc accatagccg
      361 atgccgatgt gcaaaaagga accattacac ttgtagtgca gaaaatgggc gtttcgtcga
      421 caaaactttg tgcgctgaac gaaggtgatt ttattaccga tatggtgggt ccgttaggaa
      481 aagccacgca catcgaaaat ttcggaacgg tggtttgtgc ctgtggaggt gtgggcactg
      541 cgcctatgtt gcctatagta gctgctttga aggcggccgg gaaccgtgtc attacaatta
      601 tagctgcccg aacgaaagac ttgattatcc tggaggaaca aatgaaggtg aattcggatg
      661 aggtgatagt gatgaccgac gatggcagtt atggaacaaa aggccttgtt acaaatgggg
      721 tggaagcggt gatccatcgc gagaaagtgg atctttgtgt taccatcggg ccggccataa
      781 tgatgaaatt tgtaagtaaa ctgactgaaa aatacgaagt gcctaccgtt gcttctttga
      841 atacgattat ggtggatggt accggaatgt gtggcgcctg ccgcgtaagt gtgggcggta
      901 aaaccaaatt cgtttgcgtg gatggtcctg aatttgatgc gcatttggtg gatttcgatg
      961 aaatgttgat gcgccttggt gggtacaaag atatcgaacg cgaggaaatg gaaaaaatgc
     1021 aatgcgccag tggagcggga aagaattaat gcaatgtagc cccctaaccc ccgaaggggg
     1081 aactaaacgc taattttcaa ggcaatgtac caatttgaga atatgccaat ttgagaatat
     1141 gccaatatgt caatgtgcca atttgagaat ttgaaaattt gagaatttgt cccgaaagct
     1201 ttcgggattg agaatttgtc ccgaaagctt tcgggattga agaaagagaa caggaaattg
     1261 taaattgaaa ataatccaat agaaaataag ttaaagatga atcttaccga agaaagagct
     1321 caatcatgga gagccgattt gcgcagtgct atcaaagcca aagaccgtac agcacagcct
     1381 cgtgtacaca tgcctgaact tgatgctgac tatcgcagcc gaacccgcgt ggaagaagta
     1441 aacctgggat tgacgcccga aatggccatg aaggaagcac agcgttgtct cgactgtgcc
     1501 aacccgacct gtatggaagg ttgtcctgtg agtatctata tccccaagtt tgtaaagcaa
     1561 atcgaaatcg gtgattttct gggcgctgcc cgtacattga aagaaacatc cgctttgccg
     1621 gccgtttgtg gccgtgtttg tccgcaggaa aaacagtgcg aagcgcagtg tattcatacc
     1681 aaaatgaaaa aagaagcggt ggccattggt catctcgaac gttttgctgc cgattacgaa
     1741 cgcgaaagcg gaaacatttc ggtacctgaa gtggctgcac ccaatggatt aaagattgcc
     1801 gttgttggtt caggtcctgc cggtcttgcg tttgccggcg atatggcaaa gttgggttat
     1861 gatgtgactg tttatgaagc gttgcacgag atcggcggcg tattgaaata tggtattcct
     1921 gagtttcgtt tgcccaatgc cattgtggat gtcgaaattg aaaatcttcg ggctatgggt
     1981 gtgaaattca ttaccaattg tattatcgga aaaacgataa gcattgatga tttgaaagaa
     2041 gaaggataca aaggtgtttt tgtagccagt gg
//
LOCUS       DAORKP010000248         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_20062_length_2060_cov_9.556608, whole genome shotgun sequence.
ACCESSION   DAORKP010000248 DAORKP010000000
VERSION     DAORKP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20062_length_2060_cov_9.556608"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>2060
                     /gene="dnaG"
                     /locus_tag="PLN75_09505"
     CDS             129..>2060
                     /gene="dnaG"
                     /locus_tag="PLN75_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRG04392.1"
                     /translation="MIDQLTIDRINEAAHIEDVVGDYVTLRKRGVNLLGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGKGGNSVHFIMEHEQLSYVEALKYLARKYHIEVVEKEMTPE
                     EEAARNDRESMLLVNEYAQKYFTNILHNHIDGKAVGLGYFRERGFRDDIIRKFQLGYC
                     LDEKDGFTKDALKSGYKKEFLVKTGLTIEGENNYVADRFRGRVMFPVHSLSGKVLAFG
                     GRILKKDEKLAKYVNSPESEIYHKSNELYGIFFAKQAIVRHDRCYLVEGYTDVISMFQ
                     SGIENVVASSGTSLTPGQIRLIHRFTENVTVLYDGDPAGIKASIRGIDLLLEEGLNIK
                     VLLLPDGDDPDSFARKNNASDFIRFVEENSVDFIRFKTSLLLNEVGNDPIKRAGLVQD
                     IVRSIAIIPNQLIRSEYVKECSTLLDVNEQMLYHEIAKIKNKQLESKLTPKTETETAA
                     NQQPKPEQSEKPGQTAQKFENEERNIIRVLIKYGSYLQFISTDENGENSTPVTVSDYI
                     LHELETDNLSLQNPVLDTILREYKLLPKDSATDVEKHFLNHPDVAVSQLVADLITEKY
                     TLSKIHSKFRELETDDKRLNELAPRYIYEYKLALIIQNRKEMLLEMKKAAESNDASAV
                     GQIMKEIEQIDYFKNILSKE"
BASE COUNT          650 a          438 c          479 g          493 t
ORIGIN      
        1 aggggggtaa aaaggagcaa ttttcaaatg ttttctaaaa ttcaaaattg tgttttttaa
       61 aagttatatt ttttagataa aactgtgatt accgacaaat tccccttttt gggggttagg
      121 gggcagattt gattgaccaa cttaccatag accggataaa tgaagctgcg cacatagaag
      181 atgtggtggg cgattacgtt acgctccgca agcggggtgt gaacctgctc ggtctgtgtc
      241 cgtttcacaa tgaaaagact ccgtcgttca ccgtatcacc tgcaaagggc attttcaaat
      301 gtttcggctg tggcaaaggg ggaaattccg tccattttat catggaacac gagcagctct
      361 cgtatgtgga agcgctgaaa taccttgccc gcaaatatca catcgaagtc gttgaaaaag
      421 agatgactcc cgaagaggaa gctgcccgga atgaccgcga aagcatgctg cttgtcaacg
      481 aatatgctca gaaatacttc accaatatcc ttcataatca tattgacgga aaagcagtcg
      541 gtttgggata ttttcgcgaa agaggatttc gcgatgacat tatccggaag tttcagctcg
      601 gttattgtct cgatgaaaag gacggtttta ccaaagatgc cttgaaaagt ggttataaaa
      661 aggagttttt agtaaaaacc ggtctgacta tcgaaggtga gaataattac gttgccgacc
      721 gttttcgggg ccgtgtgatg tttccggtgc actccctctc gggaaaagtg ctggctttcg
      781 gcgggcgcat cctgaaaaag gacgaaaaac tggcgaaata cgtcaattcg cccgaatcgg
      841 aaatttacca taaaagcaac gaactttacg gcattttctt tgccaaacaa gccatcgtca
      901 gacacgaccg ttgttacctg gtagaaggct acacagatgt tatttccatg tttcagagtg
      961 gtatcgaaaa tgtggtggca tcctcgggca cttcgctcac tccgggccag atccggctca
     1021 ttcaccgctt tacagaaaat gttacggtac tgtacgatgg cgatccggcg ggaatcaaag
     1081 cttcaatacg cggaatcgat ttgctgctcg aagaaggatt gaatatcaaa gtgctgctgc
     1141 ttccggacgg agacgatccg gacagttttg cccgcaaaaa caacgcctcg gatttcatcc
     1201 gttttgtgga ggaaaattcg gttgatttta tccgtttcaa aacttcgctg ctgctcaacg
     1261 aagtgggaaa cgacccgata aaaagggcag gtttggtgca ggatattgtg cggagtatag
     1321 ccatcattcc caaccaactt atacgctcgg agtatgtgaa ggaatgcagc acactgctgg
     1381 atgtaaacga gcaaatgctt tatcacgaaa tcgctaaaat aaaaaacaaa cagcttgaaa
     1441 gtaaactaac tccgaaaact gaaaccgaaa cagcggcaaa tcaacagcca aaacctgagc
     1501 aaagtgagaa accgggacaa acggcacaaa aatttgaaaa tgaagaacgt aatatcatcc
     1561 gggtactcat taagtacggt tcatacctgc aatttatatc caccgatgaa aacggtgaga
     1621 atagcacccc ggttactgtg agcgattata tacttcacga actggaaacg gataatctga
     1681 gcttgcaaaa tccggtcctt gataccattc tgcgcgaata caaactactg ccaaaagatt
     1741 cagcaacaga tgtggagaaa cattttctga atcatccgga tgtagctgtc agccagctgg
     1801 ttgccgacct gataaccgag aaatacaccc tgagcaaaat acattcaaaa ttccgcgagc
     1861 tcgaaaccga cgataagcgg ctgaatgaac tcgcaccacg atacatctac gaatacaagc
     1921 tggctctgat tatacaaaac agaaaagaaa tgctgctcga aatgaaaaag gcagccgaat
     1981 ccaacgatgc ttcggcggta gggcaaataa tgaaagaaat agagcaaata gactatttca
     2041 aaaatatact gtcgaaagag
//
LOCUS       DAORKP010000249         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_20089_length_2058_cov_6.751373, whole genome shotgun sequence.
ACCESSION   DAORKP010000249 DAORKP010000000
VERSION     DAORKP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_2058_cov_6.751373"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1217)
                     /locus_tag="PLN75_09510"
                     /pseudo
     CDS             complement(339..1217)
                     /locus_tag="PLN75_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            complement(1339..1881)
                     /locus_tag="PLN75_09515"
     CDS             complement(1339..1881)
                     /locus_tag="PLN75_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRG04393.1"
                     /translation="MIKIALTGPESTGKTELSLQLAEHFHAALEPELAREYVENLDRD
                     YTFEDVCDIARLQVEQELKYENIEDENALVIFDTDLIITKVWLDYKFGYVPPFVTERL
                     SHRFMDLYLLCEPDLPWVPDPVREHGDDRPFFYDWYEREIIQLATPYVKISGTGDDRF
                     VNARQAVEDFILAEKNKKIL"
BASE COUNT          519 a          465 c          414 g          660 t
ORIGIN      
        1 ttctacttga tgaactttct acttgatgaa ctttctactt gatgaacttt ctactgaatt
       61 atcttcccgt gtttcttttc ccatttagcc cggcatttgg cacagccgtt acggcttttg
      121 cagcctttgc atttttcaaa gaccattgta tatttgccac tcaggttggc aggtgcgtat
      181 tttacctttt ctttgcgctt ctcgtccttg tcttttactt ccctgtcctt ttctttttta
      241 tgtctttgtt ccttatctct ttttttttct tcccggtatt cctcttcctt gtgttttttc
      301 tttttcgaag catgatctcc ggtagctaaa tcgtggtatt cctcccgctt gcgttgcaac
      361 agtctggtac cgggagcaac atcggtatct acccatatat ttccaccaat aaccgagtca
      421 tcgcccactt taatatttcc caaaatagtg gaattggcgt atattatcac gttgttgccg
      481 atttttggat gccggtcaat gcctttgatg ggatttccgt tttcatccag cggaaaactt
      541 tttgctccca gcgttacgcc ctgatacatt ttcacattgc tgccaattac ggctgtttcc
      601 ccaataacga cccctgttcc gtggtcaatg gtaaagaaat cgccaataac agctccggga
      661 tgaatgtcga tacctgtttc ggaatgcgcc ttctcggtaa taatacgggg aataacggga
      721 actcccagtt tgagcagggc atgagcgatg cggtaactgc tgatagcgcg aatgcccggg
      781 taacagaaaa taacctcgcc ataacttttg gctgcaggat caccatcaaa agccgcaatt
      841 acatcggtat gaagtatttc acgtatcgcg ggcagctgac ttacaaaatc ggcggcaatc
      901 tgtatggact gtgcttcgag cgccttacag gtataatcta cagttgaatt atcgaaacac
      961 atgcccgatt taatctgctg ggtgagcaac tggtgaagca gtattatatt ctgttcgatg
     1021 tgttcgttca cattatcggg attgacatcc gaattgccga aataccccgg aaaaattact
     1081 gaacggcata gttgcactac ccgcccgata acatcggtgg atggcatagg atgattttct
     1141 tcctgctggc ggcagacgta gttcaggcta tcgggatgcg agagaagttc gatgcttttt
     1201 tcaatttgct tattcattgt gatgctgtat gactgatgta gtttgcctgt agaagtctgg
     1261 caaattatat ttttagtact gaattatcga aagaaagaac aatcgggcaa acgaactgtt
     1321 cttacaaatc ttttatttct acaaaatttt tttatttttt tctgctaaaa taaaatcctc
     1381 cactgcctgc ctggcattca cgaaacgatc atcgcctgtt ccgctgattt tcacataagg
     1441 tgttgccagc tgaatgattt cacgttcgta ccagtcataa aaaaacgggc ggtcatcgcc
     1501 gtgctctctt accggatcag gcacccaggg caaatcgggt tcacaaagca gatagaggtc
     1561 cataaagcga tgggataatc tttcagtaac aaatggcgga acatatccaa atttatagtc
     1621 gagccatact tttgtaatta tcaaatcggt atcaaaaata accagggcgt tttcatcctc
     1681 aatattctca tatttcagtt cctgttccac ctggaggcgt gcaatgtcac acacatcctc
     1741 aaaggtatag tccctatcga gattttccac atattcgcgt gccagttcgg gctccagggc
     1801 agcgtgaaag tgctctgcca gttgcaggct caattcagtt tttccggttg actccggtcc
     1861 tgtcagggca attttaatca tggaattaaa gaagttgggt cttattattc agaataaata
     1921 ttacatttgt atcagttaca atcaatcaaa aaaaggaatg cacaaaatta caacaattat
     1981 tctgctttgt tgcgcttttt tgatgacagc cacggatatt tcggcacaaa acgcagattt
     2041 taagcttctt aaaagcat
//
LOCUS       DAORKP010000250         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_20413_length_2040_cov_8.249370, whole genome shotgun sequence.
ACCESSION   DAORKP010000250 DAORKP010000000
VERSION     DAORKP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20413_length_2040_cov_8.249370"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2040
                     /locus_tag="PLN75_09520"
     CDS             <1..>2040
                     /locus_tag="PLN75_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HRG04394.1"
                     /translation="PDGNFQLNFSLSNAGEPVYQLSYKGKEVIKPSKLGLKLKNPPSF
                     TLDFTLKNKEVSSFNETWKPVWGEVSQISNHYNELLVTLEQTKQNRYMKIRFRLFNDG
                     LGFRYEFDRQPELSYFQVLDECTEFALTGDHKAFWIPGDYDTNEYYYTTTKLSEIDAE
                     KVDGTGIGTHGFFAKNAVQSPLMMKTAEGLYINIFEAALLDYSAMHLMVDQKNFVLTA
                     QLAPDATGTKVYMQTPHNSPWRTVIVSDKAADILASKTILNLNEPCKIGNTDFIKPQK
                     FVGIWWGMHVPDHWSWNYSDTPNLKLKNTDWNALKPYGRHGATTENVKRYIDFAAANG
                     IEGVLVEGWNIGWEDWAGNWKEEVFDFVTPYPDFDVKGLQAYAAGKGVKLIMHHETSA
                     SVTNYERRIDKAIEFMKENGYDAVKTGYVGWIIPRGEKHDGQWMVNHYQRVIEKMAAN
                     RIMIDQHEPVRPTGQHRTYPNLMACEAARGNEFNAWSAGNPPEHETILPFTRLMGGPM
                     DYTPGIFEIKMDYYKSGNTCQVHTTLAKQLALYVTMYSPLQMAADLIENYEKKMDAFQ
                     FIKDVAVDWDDTRYLEAEPGDYITIARKAKGTGDWFIGAITDENSRVANIDFSYLEPK
                     TWYVATIYGDAPASHWKDNPMAYEIRKVLVQNSSVLKQKLAAGGGVAISVKKGGK"
BASE COUNT          613 a          431 c          484 g          512 t
ORIGIN      
        1 cccccgacgg gaattttcag ttgaatttct cactgagtaa tgccggtgag cctgtttatc
       61 agctttcgta caaaggcaaa gaggtgatta aaccttccaa acttggtttg aaattaaaaa
      121 atcctccctc ttttactttg gattttacct taaaaaacaa agaagtcagc tccttcaacg
      181 aaacctggaa accggtatgg ggagaagtca gtcagataag caatcattac aacgagttgt
      241 tggtaacact cgaacagacc aaacaaaacc ggtatatgaa aatccgtttt cgtctgttta
      301 acgacgggct tggtttcaga tacgaattcg acaggcaacc cgaactaagt tattttcagg
      361 ttctggacga atgtaccgaa tttgccctaa cgggcgatca taaggcattc tggatacccg
      421 gcgactacga taccaacgaa tattattaca ctaccaccaa actgtcggaa attgatgccg
      481 aaaaggtgga tggtaccggc atcggtactc acggattttt tgcaaagaat gctgtgcagt
      541 cgcctctgat gatgaaaaca gctgaaggac tgtatattaa cattttcgaa gcggcattgc
      601 tcgattattc ggccatgcat ctgatggtgg atcagaaaaa cttcgttttg acagcacaac
      661 ttgcacccga tgctaccggt actaaagtgt acatgcagac acctcacaat tctccctgga
      721 gaaccgtgat agtgagtgat aaagcggcag atatcctggc ttcaaaaaca atactcaacc
      781 tcaatgaacc ctgtaaaatc ggaaataccg atttcattaa acctcaaaaa ttcgttggta
      841 tctggtgggg tatgcatgta cccgatcatt ggtcctggaa ctattcggat actccaaatc
      901 tgaaacttaa aaacaccgat tggaatgcac ttaaacccta tggacgccat ggtgccacta
      961 ccgaaaatgt gaaacgttat atcgattttg cagcagctaa cggcattgaa ggggtgttgg
     1021 ttgaaggatg gaatatcgga tgggaagact gggccggaaa ctggaaggag gaggtttttg
     1081 atttcgtgac accttatccc gattttgatg tgaaaggatt acaggcttat gctgccggaa
     1141 agggggtgaa attgattatg catcacgaaa catccgcttc ggtcaccaac tacgaaagac
     1201 gcattgataa agccattgaa tttatgaaag aaaacggcta cgatgccgtg aaaaccggtt
     1261 atgtgggctg gattattccg cgaggagaaa aacacgacgg tcagtggatg gttaatcact
     1321 atcagcgtgt gatagaaaaa atggctgcca accggataat gattgatcag catgagccgg
     1381 ttcgtccgac tggtcagcac cgcacttatc ccaatttgat ggcttgtgaa gctgcccgcg
     1441 gcaacgagtt caatgcatgg agtgccggaa atccgcctga acatgaaacc attctgcctt
     1501 ttacccgtct gatgggaggt cctatggatt acacacccgg cattttcgaa ataaaaatgg
     1561 attattacaa atccggaaat acatgtcagg tacataccac actggccaaa cagctggcac
     1621 tttatgtcac catgtacagt ccgttgcaga tggctgccga tttgattgaa aactacgaaa
     1681 agaaaatgga tgcatttcag tttatcaaag atgtagctgt tgactgggat gataccaggt
     1741 atctggaagc tgagccgggc gattatatca ccattgcccg caaggcaaaa ggaaccggtg
     1801 actggtttat cggcgccatt accgatgaaa attcacgcgt tgcaaatatt gatttcagct
     1861 acctggaacc caaaacatgg tacgtggcca ctatttatgg tgatgcacct gcttctcact
     1921 ggaaagataa tccgatggct tacgaaatcc gaaaagtgtt ggttcagaat tcttctgtgt
     1981 tgaaacagaa gcttgcagca ggagggggag tggctatcag cgtgaagaaa ggcggaaaag
//
LOCUS       DAORKP010000251         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21217_length_1998_cov_9.514668, whole genome shotgun sequence.
ACCESSION   DAORKP010000251 DAORKP010000000
VERSION     DAORKP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21217_length_1998_cov_9.514668"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLN75_09525"
     CDS             <1..293
                     /locus_tag="PLN75_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04395.1"
                     /translation="GSEQGILIDSSKTGQQNPDMWTFEVGRNMKGDNYFEINDNITLP
                     EAYRTGSFQVVVEEIELGPQSLNNIPDAYKERLEHPEETNRVVYADVFDVKR"
     gene            626..699
                     /locus_tag="PLN75_09530"
     tRNA            626..699
                     /locus_tag="PLN75_09530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Met,seq:cat)
     gene            complement(772..>1998)
                     /gene="metG"
                     /locus_tag="PLN75_09535"
     CDS             complement(772..>1998)
                     /gene="metG"
                     /locus_tag="PLN75_09535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRG04396.1"
                     /translation="KYGTWEKWWKDPETRLLHFIGKDNIVFHCIVFPAMLKAEGSYIL
                     PDNVPANEFLNLEGDKISTSRNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNDFT
                     WKDFQARNNNELVAIYGNFVNRALVLTQKYFDCKVPPIGSLNDYDKQTQDDFAGVKTQ
                     VEALLDTFHFRDAQKEAMNLARIGNKYLAETEPWKLAKTDMNRVATILNISLQIAANL
                     SIAFEPFLPFTSQRLREMLNLSTFDWKELGKMNLLAEGHQLNTPELLFEKIEDEQVEA
                     QIRKLNDTKKQNEANAFTPQAVKENVAFEDFGKLDIRVGTVLECTKVPKADKLLQFRL
                     DDGMGGRTILSGIAAYYPKPEELVGSQVLFIANFAPRKLRGIDSEGMILSAENPDGTL
                     TLVRPNEKLTNGSVVV"
BASE COUNT          539 a          413 c          458 g          588 t
ORIGIN      
        1 ccggcagcga gcagggcatt ctgattgata gttctaaaac cggccagcaa aatcccgata
       61 tgtggacatt cgaagttgga cgaaacatga agggcgacaa ctactttgaa atcaatgata
      121 acatcacact cccggaagcc taccgaaccg gttcatttca ggtagtggtt gaagaaatcg
      181 aactggggcc gcaaagtctg aataacattc ctgatgccta taaagaaaga cttgaacacc
      241 ccgaagaaac caacagggta gtgtatgcgg atgtttttga tgtgaagaga taaactgaat
      301 tcaactatca attcattcag gcaattcata gaaaacaggt ctgattctaa agaaatagtc
      361 tctttttaaa aagagactat ttcgtttgaa taatgtacaa taatccatat tgttccttct
      421 aaatgaatct ccaaaatgaa aaaagtttca gatttatttt gttataaatt tggaattaac
      481 tacctttgca cccacaaaaa atcagaaagg attgaaaacc ttaaaagatt ttattgaaat
      541 gatggcgaga tagctcagtt ggttagagcg tcccgatata aaatcgggaa ggtcgggaaa
      601 ccagctaaga tatgattgaa ataatggcga gatagctcag ttggttagag cgcatgattc
      661 ataatcatga ggtcggggga tcatgccccc ctctcgctac agaaaaagaa agcagttaca
      721 aaaaattatt tttttgtaac tgctttcttt ttattgttat gattgctaac tttaaacaac
      781 taccgagcca ttggtaagtt tttcgttagg tcgtacaagt gtgagtgttc catcgggatt
      841 ttcagctgat agtatcatgc cttcgctgtc gattccgcgc agtttgcgcg gggcaaagtt
      901 agcgatgaag agcacctgcg agccgaccag ttcctccggc tttgggtaat aagcggcaat
      961 tcccgacaaa atggtacgtc cgcccattcc gtcatccagt ctgaactgaa gaagtttgtc
     1021 ggctttgggt actttggtac actccagcac cgtgcctaca cggatgtcca gctttccgaa
     1081 atcttcgaaa gccacatttt ctttcacagc ctgaggagta aatgcatttg cttcgttttg
     1141 tttttttgtg tcattcagtt tgcgaatttg cgcttccacc tgttcatctt caattttctc
     1201 aaaaagaagt tcaggtgtat tcaattgatg accttctgcc agtaaattca tttttccaag
     1261 ttccttccag tcaaaagttg acaggttgag catttcgcgc agtctctgtg atgtaaatgg
     1321 cagaaaaggc tcgaatgcga tggaaagatt ggctgcaatt tgcagactga tgttcagaat
     1381 agtggctacg cggttcatgt cggtctttgc cagcttccag ggctcggttt ctgccaggta
     1441 tttgttcccg atgcgtgcca gattcatcgc ttctttctgt gcatcgcgga agtggaaagt
     1501 atcgagcaat gcttccacct gcgtttttac gccggcaaaa tcgtcctgag tttgcttgtc
     1561 gtaatcgttc agtgagccaa tgggcggaac cttacaatcg aaatatttct gggtaagtac
     1621 gagtgcgcgg ttcacaaaat ttccataaat ggcaaccagc tcgttgttat ttctggcctg
     1681 aaagtctttc caggtgaagt cgttgtcttt ggtttcgggt gcattggctg tgagcacgta
     1741 gcggagtacg tcttgttttc ccggaaaatc ttcgagatat tcgtggagcc aaaccgccca
     1801 gttgcgcgaa gtggaaattt tatcaccttc caggttgagg aattcattcg caggcacgtt
     1861 gtctggcagg atgtagcttc cttcggcttt gagcatggca gggaaaacaa tgcagtggaa
     1921 cacaatgttg tcttttccga tgaaatgcaa caaacgggtt tcggggtctt tccaccattt
     1981 ttcccacgtt ccgtattt
//
LOCUS       DAORKP010000252         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21316_length_1993_cov_8.107327, whole genome shotgun sequence.
ACCESSION   DAORKP010000252 DAORKP010000000
VERSION     DAORKP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21316_length_1993_cov_8.107327"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="PLN75_09540"
     CDS             <1..1517
                     /locus_tag="PLN75_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRG04397.1"
                     /translation="LFSQGYQNPVLPGFYPDPSVCRVGDDYYMVNSSFQYFPGVPVHH
                     SKDLINWKSIGHCLTRPSQLKLVKTGFSGGIYAPSIRYHNGTFYMITTNVSDKGNFYV
                     TSDDPAGEWSDPIWVDQGGIDPDIFFEEDEKTYFMSTNSGIFLCEIDIKTGKKLTESR
                     LIWNGTGARYTEAPHIYKKDGYYYLLVAEGGTEYGHKVTIARSRSLFGPYDSNPANPI
                     LTHINQNAANNPIQGTGHADFIQAHDGSWWVVFLGFRPQSYTHHVLGRETFLAPVNWE
                     EKQWPVINGDGTVALEMKVKTLPQVKNPEKPAKDEFDNDNLGFGWNFICNPNLKNYSI
                     SERKGYLRLKSSTISINEMDSPTFLGIRQQHIKFQATALLDFKNMKNGSEAGLTTYMS
                     NNYHYDLFVKKKNNRSSISLLYNLGVLKHLEKEIPVSGNKLYLRMVGGSDFYTFYYST
                     DDKNYHKTGEIDVRFLSSETAGGFTGVYLGMFAQSNVENNSYADFDWFEYKELK"
     gene            1728..>1993
                     /locus_tag="PLN75_09545"
     CDS             1728..>1993
                     /locus_tag="PLN75_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRG04398.1"
                     /translation="MASQEDVFKKLIAHCKEYGFVFPSSEIYDGLGAVYDYGQNGVEL
                     KNNIKKYWWDSMVLLNENVVGIDAAIFMHPTTWKASGHVDAFNDP"
BASE COUNT          594 a          428 c          433 g          538 t
ORIGIN      
        1 ctctcttttc tcaaggttat caaaatccgg ttttgcccgg cttttatccc gatccaagtg
       61 tatgcagggt gggcgacgat tattacatgg tcaacagcag ctttcagtat ttcccgggtg
      121 tgccggttca tcacagcaaa gacttgatca actggaaaag cattgggcac tgcctgacgc
      181 gtccatctca gttgaaattg gtgaaaaccg gtttctcggg cggaatttat gctccgagca
      241 tccgctacca caacggcact ttctacatga ttaccaccaa tgtttctgac aagggaaatt
      301 tctatgttac aagcgatgat ccggctggag agtggtcaga tcccatttgg gtggatcagg
      361 gtggtatcga tcccgatatt ttttttgaag aagacgagaa aacctatttc atgtcaacca
      421 attcgggaat tttcctttgc gaaattgata taaaaacagg taaaaagctc accgaatcac
      481 gattgatctg gaatggtaca ggcgcacgtt acactgaagc gcctcatatt tacaaaaaag
      541 acggctacta ctacctgttg gtagctgaag gaggtacaga atacggtcac aaagttacca
      601 tcgcccgaag ccgatcgctg ttcggaccct acgattccaa tcctgccaac ccaattctga
      661 cgcatatcaa tcaaaacgca gctaacaatc ccattcaggg caccggtcat gcagatttta
      721 tccaggctca cgacggttcc tggtgggtgg tttttcttgg tttccgaccg cagtcataca
      781 cgcatcatgt acttggtcgc gagacattcc ttgcaccggt gaattgggaa gaaaaacagt
      841 ggccggtgat taacggcgat ggaaccgtag cgctggaaat gaaggtgaaa acgcttccgc
      901 aagtgaagaa tccagaaaaa cctgcaaagg atgaatttga taatgataat ttgggtttcg
      961 ggtggaactt tatctgtaac ccaaacctga aaaattattc catatctgaa cggaaaggat
     1021 atctgaggtt gaaatcttca acaatttcga ttaatgaaat ggattcgccc acttttttgg
     1081 gaataaggca gcaacacatt aagtttcagg caacagcact gctcgatttc aaaaacatga
     1141 aaaacggatc agaagccggg ctgaccacat acatgtcaaa taattatcac tatgatttgt
     1201 ttgtgaaaaa gaaaaataat cgtagctcaa tctcactttt atataactta ggagttttga
     1261 aacatcttga aaaggaaatt cctgtgtcgg gaaataaatt gtacctgaga atggttggag
     1321 gaagcgattt ctacaccttt tattattcta cagatgataa aaactaccat aaaacgggcg
     1381 aaatcgacgt tcgattctta agttctgaaa cggccggtgg atttacaggc gtctatctcg
     1441 gaatgtttgc tcaaagtaat gtcgaaaaca attcgtacgc agattttgac tggtttgaat
     1501 acaaagaatt aaaataaaga gaacattcaa accgttttcg ttcagaatcc ttttcctgaa
     1561 tcctgaaatt cacatttcat aattgataac cccgcaataa gttacacgcc tggtattgcc
     1621 gtattaatca atattttttt gtaattttgc gggctgaaat attatccatc aaacagtgct
     1681 ttcctccggc atttccccaa cttgggtctc aggaggctcc ttacattatg gcttcacaag
     1741 aagatgtttt caaaaaactg attgctcact gcaaggaata cggttttgtg ttcccttcga
     1801 gcgaaattta cgacggcctc ggtgccgtgt acgactacgg acaaaacggt gtagagttaa
     1861 aaaacaacat caaaaagtac tggtgggaca gcatggtgct gctcaacgag aacgtggtgg
     1921 gcatagatgc cgccatattc atgcacccca ccacctggaa ggcttccgga cacgtggatg
     1981 cattcaacga tcc
//
LOCUS       DAORKP010000253         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21622_length_1977_cov_7.332986, whole genome shotgun sequence.
ACCESSION   DAORKP010000253 DAORKP010000000
VERSION     DAORKP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21622_length_1977_cov_7.332986"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLN75_09550"
     CDS             <1..220
                     /locus_tag="PLN75_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04399.1"
                     /translation="TDKNYFELTLSLLNSKNPVLLNFPKITKLEKLSTFDFRNIAKRE
                     KLITFDFRNITVQEKPGTFDFRNITERK"
     gene            376..738
                     /locus_tag="PLN75_09555"
     CDS             376..738
                     /locus_tag="PLN75_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRG04400.1"
                     /translation="MNEPSTHHERIARMTFASVYPLYLAKVEKKGRTREELHRVIAWL
                     TGFDDQKLQEQIDTEATFESFFNDAQLNPNANLITGIICGYRIEDIDNQLTRKVRYLD
                     KLVDELAKGRKMEKILRG"
     gene            complement(1128..1204)
                     /locus_tag="PLN75_09560"
     tRNA            complement(1128..1204)
                     /locus_tag="PLN75_09560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Asp,seq:gtc)
     gene            1419..>1977
                     /locus_tag="PLN75_09565"
     CDS             1419..>1977
                     /locus_tag="PLN75_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRG04401.1"
                     /translation="MEVTRIYDILDHYLEKYPNQEAALASKKGGVWQKISIQEYVSQT
                     NLISYGLLAIGVEKGDRVGLVSGNRPEWNMIDFAIMQIGAVSVPIYPNISQEDYRHIL
                     NHAEMKVIFIDGKDLRGKLEPIMPEVTSLKAIYTFNEEGAKYKILNDLIELGKANPQP
                     EKLEAIKAAISPDELASIIYTSGTTG"
BASE COUNT          657 a          406 c          377 g          537 t
ORIGIN      
        1 tacggacaaa aattatttcg aactcacgtt aagtttactg aactcgaaaa acccggtact
       61 tttaaacttt ccaaaaatta caaaactcga aaaactcagt acttttgact tccgaaacat
      121 tgcaaaacgc gaaaaactca ttacttttga cttccgtaac attacggtac aagaaaaacc
      181 cggtactttt gacttccgta acattacgga acgtaaatga cctcggtttt ttaatttccg
      241 aaaattttca gaatgcgaat aacctcattt tttttcactt aattgcccat tgaaagcctg
      301 tccctgcctg tcccgatttt ataccgggaa acttgattcc ggaggggcag gaatcagaca
      361 taaataaata tacacatgaa tgaaccctca acacatcacg aacgtattgc ccgaatgaca
      421 tttgcctcgg tatatccgtt gtatctggca aaagtggaga agaaaggacg aacacgcgaa
      481 gaattgcacc gcgtgatagc ctggcttaca ggctttgatg accaaaaact gcaggagcag
      541 attgacacag aagcaacttt tgagtcgttc ttcaacgatg cacagctcaa tccgaatgca
      601 aacctcatta ctggcataat ctgcggctac cggattgaag acattgacaa tcagctaacc
      661 cgaaaagtga gatacctgga taagctggtg gacgaactgg ctaaaggccg gaaaatggaa
      721 aagattttgc ggggttaaaa attggatttc atccgaaaag tgaatgacat tcttattaat
      781 taaaagaatc aacgggcaat ggcaagaagt tcaactttca tgaatttcag gaataaatac
      841 tcatttcccg ttcacttaaa tctcctgttc agctattttc ataataagtt atcaaaacaa
      901 acgcagctct tcttcccggg cagctgtttg tattgtttac tatacttttc tcgtggtgaa
      961 aaacctttgt aaaattaaag aactacagga atccggttct tgtagttctt taattttttt
     1021 ttaaacccct tgtgtgtttt gagagggaat ttgtggtgca aatgcgatgc aaatcacaaa
     1081 aaacaaaaaa tccaacaagc tgaatatcaa cttattggat ttttatctgg cggtatggac
     1141 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccaac tgaactacca
     1201 caccattgtg tttaattttg aaccgttgtt ctcaattgcg gatgcaaaga tacatcaaaa
     1261 atttgaatct gcaagaagtt ttgataaaat tatttcaaaa aaagattatt tatcgctttg
     1321 cactaaaaaa aaccgataca attttgaatt tatcatgttt ttactataaa tttacacatt
     1381 tgtaaattaa atactcattt attaaactat ttaaaacaat ggaagttaca cggatatacg
     1441 acatcctgga tcattatttg gaaaaatatc ccaaccagga agcagccctt gcatcgaaaa
     1501 aagggggagt ttggcagaag attagcattc aggagtatgt aagtcagaca aatctgatca
     1561 gttacggact attggccatc ggtgtagaaa aaggcgacag agtaggtttg gtaagcggca
     1621 accggcctga gtggaacatg attgactttg ccattatgca gataggtgcc gtttctgtgc
     1681 ctatttatcc caatatcagc caggaagatt accggcacat actcaatcat gccgaaatga
     1741 aagttatttt catcgatggc aaagacctgc gtggaaaact cgaacccatc atgcccgagg
     1801 taaccagtct gaaggcgatt tatacattca acgaagaagg tgctaaatac aaaatcctga
     1861 acgacctgat agaactgggt aaagccaatc ctcagcccga aaaactggag gccataaaag
     1921 cagccatcag ccccgatgaa cttgcctcca tcatttacac ttccggaaca acaggcg
//
LOCUS       DAORKP010000254         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21712_length_1973_cov_9.668405, whole genome shotgun sequence.
ACCESSION   DAORKP010000254 DAORKP010000000
VERSION     DAORKP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21712_length_1973_cov_9.668405"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /gene="ispG"
                     /locus_tag="PLN75_09570"
     CDS             complement(<1..1670)
                     /gene="ispG"
                     /locus_tag="PLN75_09570"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRG04402.1"
                     /translation="MWKSNPYNYKRRPTAEVRIGNLIMGGNFPVRIQSMANTNTNDID
                     NSVDQCLRIVNAGADLVRFTTQGMKEVESLKIIHQTLREKNCHIPLSADIHFNANVAD
                     FAAQHVEKVRVNPGNYVSGIKTDLTSGYTDEEYKLEYEKIRARFIPFLNICKANNTAI
                     RIGVNHGSLSERMLNRWGNTAEGMVESCMEFLHICREEDFQRVVISMKASNTVMMIQA
                     VRLLVERMESEDMHFPLHLGVTEAGDGEDGRIKSAVGIGALLAEGIGDTIRVSLSEEP
                     EAEIPVAKLLADYITQRENHPRIELKSANPFSVELYKRRKTYTIGNIGGENIPVVISK
                     PVNHASLKADYILQNETIVSSKGEIFKIYTPENFLTSKEDIVFLKMSYPELNTDIISR
                     LKNNKKTVILLETSHPNPVGEQLAFIHTLMAEGCETPVITAGKYRENDLEALQIKSAA
                     DLGVIFVDFLADGIFLDNEGIVSVEDVLSVSFGILQAARVRVSKTEFISCPGCGRTMF
                     ELQGVIASIKAKTSHLTGLKIGIMGCIVNGPGEMADADYGYVGAARGKV"
BASE COUNT          503 a          453 c          395 g          622 t
ORIGIN      
        1 acttttcccc gcgccgcacc cacataccca taatcggcat cggccatctc accgggtccg
       61 tttacaatac agcccatgat gcctattttc aggcctgtaa gatgcgatgt ctttgctttg
      121 atgctggcta tgacgccctg aagctcaaac atcgttcgac cgcatcccgg acacgatata
      181 aattcagttt tactcacccg tacgcgggca gcctgaagta tgccgaacga aacagacaaa
      241 acatcttcga ctgaaacaat tccttcgtta tccaaaaaga tgccgtcagc caaaaagtct
      301 acaaaaatca cgcctaaatc tgcagcagac ttaatctgaa gggcttcaag gtcgttttct
      361 ctgtattttc ctgctgtaat gaccggagtt tcgcaccctt cggccattag tgtatggata
      421 aatgccagct gttcacccac cggattgggg tggcttgttt cgagcaggat tacagttttt
      481 ttgttgtttt tcagccttga aattatatca gtattgagct cgggatatga cattttcaga
      541 aatacgatat cttctttcga agtcagaaaa ttttcaggag tataaatttt aaatatttca
      601 cctttagaac tgacaattgt ctcgttctgc agaatataat ctgcttttaa tgaagcatga
      661 tttaccggtt tagaaattac aaccggaata ttttcaccac cgatgttccc aatggtatag
      721 gtcttgcgcc ttttgtacaa ttctacggaa aaaggatttg ccgattttag ttcaatccgg
      781 gggtgatttt cgcgctgagt gatgtaatct gccaggagtt ttgccacagg aatttctgcc
      841 tccggctctt cgctcagcga aacgcggatg gtatcgccta tcccttcggc cagcaaagct
      901 ccaatcccca cggctgattt tattcgtccg tcttcgccat cgccggcttc ggtaactccc
      961 agatgtaaag gaaaatgcat atcttcgctc tccattcttt caaccagtaa tctcacagcc
     1021 tgaatcatca tcacagtatt cgaggctttc atcgagatga ctacccgttg aaaatcctct
     1081 tcccggcaaa tgtgtaaaaa ctccatacac gattccacca ttccttcggc tgtatttccc
     1141 caccggttga gcatacgctc gctcagcgaa ccgtgattga cacctatccg gatagctgta
     1201 ttgttggctt tgcagatatt caggaagggg ataaaacgtg cccggatctt ttcatactca
     1261 agtttatatt cttcgtcggt atagccggaa gtaaggtctg tttttattcc gctgacgtag
     1321 tttccgggat ttacccgcac tttctccaca tgctgtgccg caaagtctgc cacattggcg
     1381 ttgaaatgaa tgtctgcact caacggtatg tgacagtttt tttcgcgcaa agtctggtga
     1441 attattttca ggctttccac ttctttcatg ccctgcgtgg taaaacgcac caggtctgcg
     1501 cctgcattca ctatgcgcag gcattgatct acagaattat ctatgtcgtt ggtgttggta
     1561 ttggccatgg actgaatccg cacaggaaag ttaccgccca tgatcagatt gccgattctt
     1621 acttcagcag ttggtctgcg tttgtaattg tatggatttg atttccacat gtttatatgt
     1681 tttgtacttg ttgttttgaa atgaagttct gtaaaatgtt tcggtataat gtttaacgga
     1741 aataacattt tttcagtacc tttgcatacg ttttttttga agaaaataag ttctcagatt
     1801 gaaataaaac aatcggaaga ataaaaggtt ttacacattt ttgataaatc agcccttatg
     1861 aaagtttttg atccgaaaat tgtatataca ttaaagcatt acaccaaaga acaattttat
     1921 tccgacctca tggccggtat tattgttggt attgtagctt tgccactggc tat
//
LOCUS       DAORKP010000255         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21736_length_1972_cov_8.745957, whole genome shotgun sequence.
ACCESSION   DAORKP010000255 DAORKP010000000
VERSION     DAORKP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_1972_cov_8.745957"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLN75_09575"
     CDS             complement(<1..1096)
                     /locus_tag="PLN75_09575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRG04403.1"
                     /translation="MKIIRFTLLFVLFTYGTFTVDAQNFSPPLRIPVSLSANFGELRN
                     NHFHSGLDYRTQQSVNKPVYSIDDGFVSRINVSPGGYGLALYIDHPGGQTSVYGHLNS
                     YSKKIADYIKQKQYELESYRIDIELKPNEITVKKGEQIALSGNSGSSGGPHLHFEIRD
                     TKTQDPLDAKDFLGKTMTDTQKPDIRGIAFYPQEGKGVINGSSNPIRLNIGKTKSGDY
                     GGLGKTIHAWGTIGMGVKAYDRMNETGSVLGVENVALFVDGVPVFRSSMNRFPFDKTR
                     MINSFVDFEDWRNNKSFFMKSFVEPGNTLTLYETINNGYINITEERIYNLRYELSDHY
                     GNKTTYSFVVKGQKQAIPPVPVCDNRMSVLL"
     gene            complement(1470..>1972)
                     /locus_tag="PLN75_09580"
     CDS             complement(1470..>1972)
                     /locus_tag="PLN75_09580"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRG04404.1"
                     /translation="LELRNTCNDVVEPYAEMFGAKFVANRKPWEVKVDIAMPCATQNE
                     LNGDDAKFIVANGTKYVAEVSNMGCTPEAIDMFIENKVTYGPGKAVNAGGVATSGLEM
                     TQNAMHISWPAEEVDAKLKQIMNDIHNQCTRYGKENDGYINYMKGANIAGFMKVAKAM
                     LAQGVL"
BASE COUNT          502 a          440 c          408 g          622 t
ORIGIN      
        1 ttaacaatac agacatacgg ttgtcacata caggcaccgg aggaatcgct tgtttttgcc
       61 cttttacaac aaatgaatag gtagttttat tgccataatg gtctgagagc tcatagcgga
      121 ggttgtaaat ccgttcttcg gtgatgttga tgtatccgtt gttgattgtt tcgtacaaag
      181 taagtgtatt gcccggctcc acaaatgatt tcataaagaa cgatttattg ttgcgccagt
      241 cttcgaaatc gacaaaggaa ttgatcatcc gagttttgtc aaacgggaaa cgattcatgg
      301 aacttcggaa aacaggcaca ccatccacaa aaagagcaac attttccacg cccaacacac
      361 ttccggtttc gttcatgcgg tcatacgcct tcactcccat cccaattgtt ccccaggcgt
      421 gaatggtttt acccaatcct ccgtaatcgc ctgattttgt tttcccaatg ttcaaccgaa
      481 tcggattgct gcttccgttt attacccctt ttccttcctg cggataaaaa gcaattcccc
      541 gaatgtcggg tttctgagta tcggtcatcg tttttccgag aaaatctttg gcatccagcg
      601 gatcctgggt tttggtgtcc cggatttcga aatggagatg aggtcctccg gagctacccg
      661 aattgccgct cagggctatc tgttcgccct ttttcacagt aatttcattc ggttttagtt
      721 caatatctat ccggtaagat tccagttcgt actgtttttg tttgatataa tcggctattt
      781 ttttggaata actgttgaga tgaccgtaaa cgctggtttg cccgccggga tgatcgatgt
      841 acaatgccaa cccgtaaccg cccggtgata cgtttatgcg tgaaacgaat ccgtcatcaa
      901 tggaataaac cggtttgttg acgctttgct gcgttctgta atccagcccg gagtggaaat
      961 gattgtttct taattcacca aaattggcac tcagtgatac cggaatgcgt aatggcggtg
     1021 aaaagttttg agcatcaacc gtaaaagttc catacgtgaa gagcacaaaa aggagtgtaa
     1081 atcgaattat tttcattttt ccggtgttta tatcagcccc ctacccctcc cgaatcaagt
     1141 ttcccgatat aaaatcggga ctggccggga caagctctca agagggaata ataagttttg
     1201 atttggcttt gtgaataatg ttgtgtctat ttaaatattt ccttcccgat tttcatcgaa
     1261 acaagttgtg tgtcctgttt tttttaaatc aggtcattat aatttcatct tttaaaaaag
     1321 tttattttca ggcttaacaa cggaaaagcc cattttgttg agtgttgcag aaatccttcc
     1381 ggtttatttc taaaaaaaac cgaccctgca tatcacaagc aaggccggtt caaaaagata
     1441 aaaattatat agtaaatgat aaaagtattt tacaacacac cctgtgccaa catagctttg
     1501 gccactttca taaatccggc aatattggcg cctttcatgt agttgatgta cccgtcgttt
     1561 tccttgccat agcgggtgca ttggttgtgg atgtcattca tgatttgctt caatttggcg
     1621 tccacttctt cggcaggcca ggaaatgtgc atggcattct gcgtcatttc caatccggaa
     1681 gtagctacac cgcctgcatt aacggcctta cccggtccgt aggtcacttt gttttcgata
     1741 aacatatcga ttgcttcggg agtacagccc atgtttgaaa cctcagctac gtattttgtc
     1801 ccgttggcaa caatgaattt tgcatcatca ccgttcagtt cattctgtgt agcacatggc
     1861 atggcaatgt ccacttttac ttcccacggt ttacgattgg ctacaaattt tgctccgaac
     1921 atttctgcat aaggttctac cacgtcgttg caggtattgc ggagttcgag ca
//
LOCUS       DAORKP010000256         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_21923_length_1963_cov_8.888365, whole genome shotgun sequence.
ACCESSION   DAORKP010000256 DAORKP010000000
VERSION     DAORKP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21923_length_1963_cov_8.888365"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLN75_09585"
     CDS             complement(<1..956)
                     /locus_tag="PLN75_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRG04405.1"
                     /translation="MPPFAHIDCNNFYASCERVFNPSLIGRPVIVLSNNDGCAIARSN
                     EAKALGVKMGEPAFKIKELIEEHNIAVFSANFELYGDMSNRVMTILSGFSPSQEIYSI
                     DECFLDLEGINVDLRAYGLEMKQKVEKWTHIPVSIGIGPTKALAKTATRVAKKFTPQT
                     GGVHFIDTDEKRIKALKWLQIEDVWGIGRQYTKKLTAMNIRNAYQFVNLSDEWVKTHL
                     TIAGLRLKHDLQGIPTLQLDEVKNKKSIATTRTFDEMIENKADIRERVSTFAVKCAEK
                     LRKQKTCCNAIQVFITTNYFREDLPQYSNSVTIKLPFATNSS"
     gene            complement(956..1414)
                     /gene="umuD"
                     /locus_tag="PLN75_09590"
     CDS             complement(956..1414)
                     /gene="umuD"
                     /locus_tag="PLN75_09590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRG04406.1"
                     /translation="MKEMKSPKPIITTPGFDIYKADAASEMLLPYIGSLRAGFPSPAD
                     DFTGERIDLNRLCINHPEATYFARAKGSSMNRDFNEDDLLVIDSSLEYKNNYIAMIFI
                     DGDFTSKRIRKIRNKCYLESSNSEFPPIEITEESKNLIFGIITWSFRKHL"
     gene            complement(1441..>1963)
                     /locus_tag="PLN75_09595"
     CDS             complement(1441..>1963)
                     /locus_tag="PLN75_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRG04407.1"
                     /translation="LLYEFKDGLNNYFASLGADAPVKNMEELIRLQTADSTEMEWFDH
                     QLFEMAQAKGSVDSDAYKKLLTDMLKGARENGIDKIMNENRLDAIVAPTGSPGWKTDL
                     VNGDNFGISSSSPAAIAGYPAISLPMGFVAGLPVNITFYGKAWSEPNLISMAYTFEQG
                     TKHRKQPTFKTGE"
BASE COUNT          475 a          433 c          451 g          604 t
ORIGIN      
        1 atcgacgaat tggtggcaaa gggcagtttt attgtaacag aattgctgta ctgaggcagg
       61 tcttccctga aataattggt tgtaatgaac acttgtatgg cattgcagca ggttttttgt
      121 tttcgcagtt tttcggcaca tttcaccgcg aaagtagata cccgctccct gatatcggct
      181 ttattttcaa tcatttcgtc gaatgtacga gtcgtagcaa tgcttttttt gttttttact
      241 tcgtccagct gaagcgtggg gataccctgc aggtcgtgtt tcaggcgaag tcccgcaatg
      301 gtcaagtgag ttttcaccca ttcatcggat aagtttacaa actgataggc attccggata
      361 ttcatggctg tcagtttttt ggtatattgt cggcctatgc cccacacatc ttctatctga
      421 agccatttga gtgctttaat ccgtttttcg tcggtatcga tgaagtgaac accacccgtt
      481 tgaggtgtaa attttttagc tacgcgtgta gcagttttgg caagtgcttt ggttgggcct
      541 ataccaatgc ttaccggtat gtgggtccac ttttccactt tctgtttcat ttccagcccg
      601 taagccctca agtcgacgtt aataccttcc agatcgagaa aacactcgtc gatgctgtaa
      661 atttcctgcg agggtgagaa accactcaga atagtcatca cacggttgct catatcgcca
      721 tagagttcga aattggccga aaaaacggca atattgtgtt cttcaattaa ttccttgatt
      781 ttgaaagcag gctctcccat tttcacaccc agcgcttttg cttcgttgct acgcgcaatg
      841 gcgcagccgt cgttgttact gagcacaatt acaggcctgc cgatgagcga cggattgaaa
      901 acccgctcgc aggaggcata gaaattatta cagtcgatat gtgcaaacgg tggcattaca
      961 agtgctttct gaaactccag gtgatgatgc caaaaatcag atttttcgat tcttcggtta
     1021 tttcgatggg tggaaactcg ctgttggatg attccaggta acatttattc cgtatctttc
     1081 tgattcgctt ggaagtaaaa tcgccgtcga taaaaatcat ggcaatgtag ttgtttttat
     1141 attccaaact cgaatcgatg accagcaaat cgtcttcatt gaaatccctg ttcatggaag
     1201 aacctttggc acgggcaaaa taagtagctt cgggatgatt gatacacaac cggttaaggt
     1261 cgattcgttc gccggtaaaa tcatcggccg gtgacgggaa acctgcgcgg agtgaaccga
     1321 tatagggtaa aagcatttcg gaagctgcgt ctgctttgta aatatcaaag ccgggtgttg
     1381 taatgatggg ttttggcgat ttcatttcct tcattcattc attttttacg aattgacaca
     1441 ttattcaccg gttttgaaag tgggttgttt ccggtgtttg gtaccttgct caaatgtata
     1501 ggccatgctg ataaggttgg gttcgctcca cgcttttccg tagaatgtga tattcaccgg
     1561 aagtccggct acaaagccca tgggcagact aatagcggga taaccggcaa tggctgccgg
     1621 agaactggac gaaattccga agttgtcgcc attcaccaga tcggttttcc agccaggcga
     1681 accggttgga gcaacaatgg catccagcct gttttcgttc attatcttat ctattccgtt
     1741 ttcgcgggca cctttcagca tatcagtaag cagctttttg tacgcatccg aatccactga
     1801 acctttcgcc tgagccattt caaataattg atgatcaaac cattccatct ctgtgctgtc
     1861 agcagtttgc agccggataa gttcttccat gttttttacc ggtgcatccg ctccgagtga
     1921 agcgaaatag ttattcagcc cgtctttgaa ttcatagagc agg
//
LOCUS       DAORKP010000257         1931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_22596_length_1931_cov_6.695629, whole genome shotgun sequence.
ACCESSION   DAORKP010000257 DAORKP010000000
VERSION     DAORKP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22596_length_1931_cov_6.695629"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLN75_09600"
     CDS             complement(<1..528)
                     /locus_tag="PLN75_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRG04408.1"
                     /translation="MAGDSTMADKVLTKTVMDSLTGTTTEVPFLERGWGQLLPEFINR
                     KAIVKNYAKNGRSTRTFIEEGLWTDLLANTRKGDVVIIQFAHNDGAVTKTERYTNPAQ
                     FRLNFIAFVAEVKAKGAIPILCTPVARRKFNEKGELVPTHGEYPEIIRAVAREQRVAL
                     VDMEKLTSGWLQKEGV"
     gene            complement(642..>1931)
                     /locus_tag="PLN75_09605"
     CDS             complement(642..>1931)
                     /locus_tag="PLN75_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HRG04409.1"
                     /translation="AQAAQASGNRFYIFFPDGEYNIGENTGDANQMTTISIPRLSLIG
                     QSSNVLVYNQSIQEAINTTATMYFTSSSSDNYMQDIILRNKMDFRTGTLKGRGIALWD
                     QGNKNIYKNVKLQSNQDTYYSGNVIRSYFENCDIHGTVDFLCGGGDIFFNECLIYLEE
                     RSGNVITAPSTSTDWGYVFNNCTIDGFPVNNNGYRLGRPWSNAPKAVFLNTTMKVLPV
                     SAGWGDPMNVVPAVFAEYNSMTTNGAAIDLSGRRTTYTKDATTVVLNPVLSAEQAASY
                     TIENVLGGTDTWQPKLYTDQAAAPVIAGDERVISWTNSDYVLCWAVFRNNQFVKFVTT
                     NSYTIPEETTSGSTYTVKAANEMGGLSQSSNPYEYVKTGIFTQPENTALIDRKFYSAE
                     GKQIINLTGYSGFIIVRSVYADGSVITDKILKATPKR"
BASE COUNT          495 a          440 c          413 g          583 t
ORIGIN      
        1 cacaccttcc ttttgcagcc atccggaggt gagtttttcc atatctacga gcgccactcg
       61 ctgctcacgg gctacagccc tgattatttc aggatattcg ccgtgtgtgg gtacaagttc
      121 acctttttcg ttgaattttc ttcgggctac cggagtgcag agtatcggaa tagctccttt
      181 tgccttcact tcagctacaa aagcaatgaa attcagccga aactgcgccg gattggtata
      241 tcgttcggtt ttggtcactg ctccatcgtt gtgagcaaac tgaataatca ccacatcacc
      301 tttacgagta ttggcgagca ggtcggtcca cagtccttct tcgataaatg tgcgggtgct
      361 ccggccgttt ttggcgtaat tcttcactat agctttccgg ttgataaatt cagggagcaa
      421 ctgaccccag ccgcgctcca aaaaaggaac ttctgtggtc gttccggtca gactgtccat
      481 tacggttttt gtcagcactt tatcggccat ggtggaatcg ccggccatga gaatacgtac
      541 gggctttgag ctgaaactta tgaaacataa accaactaca aaaaacaata tatacttcaa
      601 tttcagatac atacgtttat catttacaaa tgttacatca attatctttt aggcgtagct
      661 ttcagtatct tatcagtaat cacgcttccg tcagcataaa ccgaacgaac gatgataaat
      721 ccgctgtaac cggttaaatt gataatctgc ttaccttctg cactgtagaa ttttctgtca
      781 attaaagcgg tattttcggg ctgagtgaaa attccggttt tgacatattc atacggattt
      841 gaactttgac tcaatcctcc catttcattg gctgctttca cagtataagt tgagcctgaa
      901 gttgtttctt cgggtattgt atagctgttg gtggttacaa acttcacaaa ctgattgttt
      961 cggaacacag cccagcaaag gacgtaatcg ctatttgtcc aggaaatcac acgttcatca
     1021 cctgcaataa ccggtgcagc agcctggtca gtgtagagtt tgggttgcca tgtgtcggtt
     1081 ccgcccaata cattttcgat ggtatatgaa gcagcttgtt cggcagataa caccggatta
     1141 agcacaacag tagtagcatc tttggtgtaa gttgtacggc gtccgcttaa atcgatagct
     1201 gcgccattgg ttgtcatact gttatattcc gcaaatacgg caggtaccac attcatcggg
     1261 tcaccccagc cagctgaaac cggaagtact ttcattgtag tgttcagaaa aactgcttta
     1321 ggggcattgc tccacggacg tcccagtctg taaccgttgt tgtttacagg aaagccatca
     1381 attgtacagt tattgaaaac atagccccaa tcggtactgg ttgacggagc agtaataaca
     1441 tttccgcttc tttcttcaag ataaatcaga cattcgttga agaaaatatc gccgccaccg
     1501 caaaggaaat ccacagtgcc gtgaatatcg cagttttcga aatacgaacg gattacattt
     1561 ccgctgtaat aggtatcctg attactttgc agttttacgt ttttgtaaat atttttattt
     1621 ccctgatccc atagcgctat accgcgtcct ttaagcgtac cggtacggaa atccatttta
     1681 ttgcgcaaaa taatatcctg catgtagttg tccgaactgg aacttgtgaa atacatggta
     1741 gctgtggtgt taatagcttc ctgaatggat tggttgtaca ccagtacatt gctgctttgc
     1801 cctattaacg acaacctggg aatgctaatc gtggtcattt ggttggcatc gcccgtgttt
     1861 tctccaatat tgtattcgcc atcggggaag aaaatataaa aacggtttcc gctcgcttgt
     1921 gcagcctgag c
//
LOCUS       DAORKP010000258         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_22718_length_1925_cov_7.826203, whole genome shotgun sequence.
ACCESSION   DAORKP010000258 DAORKP010000000
VERSION     DAORKP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22718_length_1925_cov_7.826203"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLN75_09610"
     CDS             complement(<1..737)
                     /locus_tag="PLN75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRG04410.1"
                     /translation="MATEPKTEKEITVEEKLKALYELQKVDSKIDEIKILRGDLPLEV
                     QDMEDEIVGLNTRLENYETEIAQISTMITGKKVEIEEAKIKIERYKEQQNNVRNNREF
                     DNLSKEIEFQTLEIELSEKRIREFTASLENKKNEKNETADQLSERKLDLNQKKAELED
                     IVSETKQEEEKLRERAKKIESMIETRLVTAFKRIRKNARNGLAVVSVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDL"
     gene            complement(742..1836)
                     /locus_tag="PLN75_09615"
     CDS             complement(742..1836)
                     /locus_tag="PLN75_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRG04411.1"
                     /translation="MTVREITSLIEDAAPLALQENYDNAGLLVGNHEMEVSGVLLSID
                     ITEAVIDEAVQHDCNLIISHHPLIFRGLKNLTGQDYIQRCIIKAIKNDISIYAAHTNI
                     DNVLNGVNGKIADKIHLQKRSVLIPKTGMLLKLATFVPTGYADIVRNALFNAGAGQIG
                     EYDSCSFNTNGTGTFRPSDNANPFAGQPNEFHTEFETRIEVILPVFLKTRVVNALIQS
                     HPYEEPAFDIIPLLNTWNKVGSGIVGELKHETDEMDLLMELKNAFALETLRHSPLHGK
                     KIKKVAICGGAGSEFIPDAISKGADVFISGDIKYHDFFNGENKILIVDIGHYESEQYT
                     KDVFYEIITKKIPNFAVRISEVNTNPIKYL"
BASE COUNT          506 a          393 c          336 g          690 t
ORIGIN      
        1 aggtcaacaa gaattcggcc gcaatattcg catacaataa tttttttgcg ggatctgata
       61 tcgagctggc gctgagccgg gattttatta aagcagccac cgcaggcatc gcgctgaaca
      121 gacactacag ccaaaccatt acgggcattc ttgcggattc gtttgaaagc tgttaccaaa
      181 cgcgtctcaa tcattgattc aattttcttt gcacgttctc gaagcttttc ttcttcctgc
      241 tttgtttcag atacgatgtc ttccagttcg gctttcttct gattcaggtc tagcttacgt
      301 tcagacaact gatcggcagt ttcatttttt tcgttctttt tgttttcaag tgaagcagta
      361 aattcacgaa tacgtttttc tgacaattcg atttctaatg tctggaattc aatttctttt
      421 gacaggttat caaattcacg attattccga acattgtttt gctgttcttt gtatcgttca
      481 attttaattt tggcttcttc aatttccact tttttgccgg taatcatcgt actgatttga
      541 gctatttcag tttcatagtt ttccaggcga gtgttcaaac ctactatttc gtcttccata
      601 tcctgaactt caagcggaag atcgccgcgt agaattttta tttcatcaat tttcgaatcg
      661 actttttgaa gctcataaag ggctttcagc ttttcttcaa ccgtaatttc tttttcagtt
      721 ttgggttcag tagccatata gttataaata ttttattgga tttgtgttca cttccgaaat
      781 tcggactgca aagttaggaa ttttttttgt aattatctca taaaaaacat cttttgtgta
      841 ttgttcgctc tcgtaatgac cgatatcaac tatcaaaatt ttgttttctc cgttaaaaaa
      901 atcatggtat ttaatatctc cggatatgaa tacatcggct ccttttgata ttgcatcggg
      961 tatgaattcg cttccggctc caccgcaaat ggctaccttt tttatcttct taccatgtaa
     1021 aggtgagtga cgcaaggttt caagagcaaa tgcatttttc agttccatca ataagtccat
     1081 ttcatctgtt tcgtgcttca actctcccac tattccggat ccaactttat tccaggtatt
     1141 taaaaggggt atgatatcga aagccggttc ttcgtatgga tgagattgaa tcaatgcgtt
     1201 cactaccctt gtttttaaaa atacgggtag aatgacttct attctagttt caaattcggt
     1261 atgaaactca tttggttgtc ctgcaaatgg attggcatta tctgatggcc gaaaagtgcc
     1321 ggtaccgttg gtgttaaaac tgcaactgtc atattcgccg atttgtcctg caccagcatt
     1381 aaataaagca ttccgaacaa tatccgcata tccggtcgga acgaaagtcg ccagtttaag
     1441 aagcatacct gtttttggaa tcaataccga acgtttctgc agatgaattt tatcggcaat
     1501 ttttccattt acaccattca aaacattatc tatgttggta tgagctgcat agattgaaat
     1561 atcattctta atggctttaa tgatacaacg ctgaatgtaa tcttgtcctg ttaaattttt
     1621 taatcctctg aaaatcaatg gatgatgaga aataatcaag ttacagtcgt gttgaacagc
     1681 ttcgtcgata actgcctcgg taatatcaat ggacagcaaa actcctgaaa cctccatttc
     1741 gtggttaccc accaacaaac cggcattatc atagttctcc tgcaaagcca gtggagctgc
     1801 gtcttcaatc agagatgtaa tttctcttac tgtcatcggt ttaatcagaa attatgttcg
     1861 tttttttgca gaaaacttaa ccttttccct tttcagaaaa atcatttttt ccggtacttc
     1921 attgc
//
LOCUS       DAORKP010000259         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_23119_length_1906_cov_6.841707, whole genome shotgun sequence.
ACCESSION   DAORKP010000259 DAORKP010000000
VERSION     DAORKP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23119_length_1906_cov_6.841707"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..993)
                     /locus_tag="PLN75_09620"
     CDS             complement(208..993)
                     /locus_tag="PLN75_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRG04412.1"
                     /translation="MKQSEEVRKLLDEAVIRYNQPDFIEYDPIQIPHRFTRKEDIEIA
                     GFLAATLAWGQRKTIVKNGDFLMDLMENSPFDFVKEGKDFNRFSRFVHRTFNGADCVF
                     FLNSLKNIYQNYGGLEQVFTDGFQKNKTIYDSLKHFRDVFFSIPHEKHAEKHVSDVAS
                     GSSAKRLNMYLRWMVRKDEQEVDFGLWKGIPASALMLPLDVHTGEVSRALGLLNRKQN
                     DWKAVEEITGILRTFDAQDPIKYDFALFGMGIEAHPILPNGGL"
     gene            complement(1019..1591)
                     /gene="pnuC"
                     /locus_tag="PLN75_09625"
     CDS             complement(1019..1591)
                     /gene="pnuC"
                     /locus_tag="PLN75_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRG04413.1"
                     /translation="MNYLEISAVIFGIASVWYARKENILVFPFGIISVLIFIYIFFVG
                     KMYANAGINAVYFLTNMYGWYNWSRKTDDEHVVKITRNTPKQNLLVILFAVIFYAAVL
                     FLLRWYNRADIEYINSSKSWMDALNSTIFMCATILMTVKKLENWHFWLLGNVISIPIF
                     LVQGYYFVAFQYSVFLILAISGLKEWKKKV"
BASE COUNT          557 a          400 c          345 g          604 t
ORIGIN      
        1 cggatttgag atgtggtgaa tattttcata aagtacgaat tacacgaatt tatacgaatt
       61 acacgaattt tgcgaaaaat gttgatcgga aaacttttca acttattaac atccaaacat
      121 ccgaacatcc aaacatccga acatccaaac ccgaacatcc aaacccgaag gggaggatta
      181 atatgtattg tgagtttttt aactttgtca tagccctcca tttgggagga ttgggtgggc
      241 ttcaattccc attccaaaga gtgcaaagtc atattttatc ggatcctgtg catcaaatgt
      301 acgaagaatt ccggttattt cttccacggc tttccagtcg ttttgcttcc ggttcagtaa
      361 tccgagtgcc cggctgactt cgcccgtatg cacatcgagt ggaagcatca gagccgatgc
      421 cgggataccc ttccaaagac cgaaatccac ttcctgttcg tcttttctga ccatccaccg
      481 gaggtacata ttcagccttt tggccgatga tcctgaagct acatccgaaa catgtttttc
      541 tgcgtgtttt tcgtgtggaa tactgaagaa aacatctctg aagtgcttca gtgaatcata
      601 tattgtttta tttttctgaa atccatctgt aaaaacctgt tctaagccac cgtaattttg
      661 ataaatattt ttcaatgaat tcagaaaaaa tacgcagtct gcaccgttaa aagttcggtg
      721 tacaaagcgg ctgaagcggt taaaatcttt accttccttc acaaaatcga agggcgaatt
      781 ttccatcaaa tccatcagaa aatctccgtt ttttacaatg gtttttcgtt gtccccaggc
      841 aagtgttgca gccaaaaatc cagctatttc gatgtcctct tttcgcgtaa accggtgcgg
      901 aatctgaatt ggatcgtact caataaagtc cggttgattg tatcggatta ccgcctcgtc
      961 aagcagtttc ctgacttcct ctgattgttt catccaaaat tcggttttaa gctgtgcgtc
     1021 acactttctt tttccactct ttcagtccgg aaatggccag aatcaagaat actgaatact
     1081 gaaaagccac aaaataatag ccttgcacca aaaaaatcgg aatggaaatc acgtttccta
     1141 aaagccagaa atgccagttt tccagttttt ttacagtcat caaaatcgta gcacacatga
     1201 aaatggtgct attcagtgca tccatccatg attttgagga gttgatgtat tctatgtcag
     1261 cacgattgta ccaacggagc aaaaacaaaa ctgctgcata aaaaatgaca gcaaaaagta
     1321 tgacaagcag attttgtttg ggtgtgtttc gtgtgatttt taccacatgc tcatcgtcgg
     1381 ttttacgcga ccagttgtac cacccataca tattagtcag gaagtaaacc gcgtttattc
     1441 ctgcgtttgc atacattttt ccgacaaaga aaatgtagat aaaaatcagc acactgataa
     1501 tgccgaaagg gaatacaagg atattttcct tacgggcata ccatacgctg gcaattccga
     1561 aaattacggc tgatatttct aaataattca tgatgatact gtttttttgc tactgaactc
     1621 gtcctgactt ttaaaaacgt agagaacaaa agtcttattt tatcggtttt tttatgctat
     1681 tttaattttt ttcgagaaca atagtttaaa aatatcgaac ctgccttgtt tatcccgaca
     1741 tgtcggggcg gcaggcaggg atatttttct ctatgtttcc gaaaatatga gaatttgtat
     1801 aaattattct atgttttcta ctgtttcaaa aaaagtcaag ttcacttcaa tgattatttt
     1861 ccggtttcat gattaccccc tcatttttcc ggccatccac tttaat
//
LOCUS       DAORKP010000260         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_23547_length_1886_cov_10.040961, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000260 DAORKP010000000
VERSION     DAORKP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23547_length_1886_cov_10.040961"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1886)
                     /locus_tag="PLN75_09630"
     CDS             complement(<1..>1886)
                     /locus_tag="PLN75_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04414.1"
                     /translation="IYLPDPMAAGIAIFADTESENTHTEDFSPRMFSFFTKEELTPDN
                     TNSVNIPEDWYDTQAIRIKLEEGALAGNWDGTKRTFTVKLPKGQRMKLKISSFWREED
                     MKKLSAVWQLVKSDAGSKLAEIEKIAKSGQHWMITPSRTFELVHAVQQPVEAPALLQV
                     VPERDYDDTSAFLNVRFNVHGESTDSAELKASWTEPLDDGISVEIKEKSFNDSIPEID
                     IYYHDKTVTVGTINDPKPGLKPDRLQLREPRWKTEPASIRQFDVQPGSLKINNIYKFQ
                     AEKLASVQQQKAAEPSRNVVNTLRFDLEESRLNLVKMLDLRHYPLEHAFGNTRHRWVN
                     YSIVASSRYSAYFTNIQKKKPEISFVRESNVGKVNILSTARPDKPEIDYVIPAFEWQK
                     TNQGGVMTHIRKGGVLRVYLKRPWFSSGEEEKLAVVLPDSSGTASLVTTMVQSPVYTD
                     IYTHWGADPLFVSELPGKSSPLPADFRYNPVVEKGLVYPGKEDVKATAVAYPVTFDRE
                     KQLWYADIALNTANMYFPFIKLALARYQAHSLRENNTDVCLSNVVMGGMIQMVPERKA
                     SIGLSQAGKNTVMNFRLSGPTYSHKSSKFGIREVIKVTVMQTDSTGSEQGILIDSSKT
                     GQQNP"
BASE COUNT          446 a          449 c          445 g          546 t
ORIGIN      
        1 ggattttgct ggccggtttt agaactatca atcagaatgc cctgctcgct gccggttgaa
       61 tccgtttgca tgacagtgac ttttatcact tcacgaattc caaatttgga cgatttatga
      121 ctgtaagtcg ggccgctgag cctgaaattc attaccgtgt ttttccctgc ttgcgacagg
      181 ccgatggaag cttttcgctc cggcaccatt tgaatcatgc ctcccatgac cacattcgac
      241 aggcaaacat ctgtattatt ttcgcgaagc gaatgggcct gatagcgggc taaagccagt
      301 tttataaacg gaaaatacat gttggcggta ttcagcgcaa tatcggcata ccacaactgc
      361 ttttccctgt cgaacgtaac gggataagcc acagcagtag ctttcacgtc ctcttttccg
      421 ggatatacta atcctttttc caccacagga ttgtacctga aatcggcagg gagcggactt
      481 gattttccgg gaagctccga aacaaacaac ggatcggcgc cccagtgtgt gtaaatatcg
      541 gtataaacag gactttgaac catcgtggtg accaaactgg cagttccgga agaatcggga
      601 agtacgacag ccagtttttc ttcttcgccg cttgagaacc acggacgttt gaggtacacc
      661 cgaagcactc cgcctttacg aatatgagtc attacgcctc cctgattggt tttttgccat
      721 tcaaaagcag gaattacata atctatttcg ggtttatcgg gccgggcagt gctcagaatg
      781 ttcactttac caacgttgga ttctctgaca aatgaaatct ccggtttttt cttctggata
      841 ttggtaaaat acgcgctgta acgcgacgat gccacgatgg agtaatttac ccagcggtga
      901 cgtgtattgc cgaaagcatg ttcaagcgga taatggcgta aatcaagcat tttgaccaga
      961 ttcagccggg attcttccag gtcaaatcgc agcgtattca ccacatttcg cgaaggttcg
     1021 gctgcttttt gttgctgaac gctggccagt ttttcggcct ggaacttata gatattgttt
     1081 attttgagcg aaccgggctg tacgtcaaat tgccggatag aagccggttc ggttttccag
     1141 cgcggttctc tcagttgcag tctgtccggt ttcaggccgg gtttggggtc atttattgtt
     1201 ccaacggtca ccgttttgtc gtggtaataa atatcaattt cgggaattga atcgttgaaa
     1261 cttttttctt ttatttccac agaaatgcca tcgtcgagcg gttcggtcca gctggctttc
     1321 agttcggcag agtcggtact ttctccgtga acattaaacc ggacattcag aaaagcggaa
     1381 gtatcatcgt aatcgcgttc gggtacaacc tgcagtaaag caggcgcttc aaccggttgc
     1441 tgcacagcat gtacaagttc aaacgtgcgc gagggagtaa tcatccagtg ctgaccgctt
     1501 ttggcaattt tctcgatttc agccagtttt gagccggcat cacttttcac cagctgccaa
     1561 acggccgaaa gctttttcat gtcttcctca cgccagaaag agctaatttt caacttcata
     1621 cgttgtcctt tgggcaattt gacggtaaac gtacgctttg ttccgtccca attacctgcc
     1681 agggcacctt cttccagctt tatccggatg gcttgcgtat cgtaccagtc ttcgggaatg
     1741 ttcacgctgt tcgtattatc gggagtgagt tcttctttgg tgaaaaaact gaacatacgc
     1801 ggcgaaaaat cttcggtatg cgtattttca ctctctgtat cggcaaatat ggctattccg
     1861 gcagccatcg gatcgggcag ataaat
//
LOCUS       DAORKP010000261         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24073_length_1862_cov_7.809629, whole genome shotgun sequence.
ACCESSION   DAORKP010000261 DAORKP010000000
VERSION     DAORKP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24073_length_1862_cov_7.809629"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="PLN75_09635"
     CDS             complement(<1..1841)
                     /locus_tag="PLN75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRG04415.1"
                     /translation="MKLRLRFTLLLLFLYANISAYTPKAEAYLQQYRKAASNETGLKA
                     LLDFCGEYKTFPYDTLKKYASEALLLSRRLQNEDSELQSKFWLATYFYRTSQLDSALN
                     ICRQLEKSSIRDVSLDRKNRLLISSVLIKKGQNQEAISRYFSLLDESDIHSDTLSYLS
                     AMHGLGWANMEIQRTDEAKKWLLSVLNLPVSSRLEEIKIPSYINLSVIYGMQNNLDSA
                     NVLIQKAIELSVKFNNLLNEANSYNILANIYSARGLNQKAIDAVKKSMQLRLHIGDPF
                     YVVSDLAQLSGLYAETGNYAEGINLAQKAWEMTGENGLASKKPIVLDALFKNYYGSGN
                     YKKSTETLLLLEKIKDSTNIAISAEKLSEMEAKYELSKKEITIKNQESEINRKNLIIW
                     FAIISLIGIILTGVVIYRSLQYIQQSKIKSIELEQQQKLTQSIFETEENEKTRIGASL
                     HDGLGQLLSAIKMNMQFMQTHLNPDDPKVNEVYHKTLDLVETSIRETREVSHELVTNF
                     IITKGFKTAVEDLVNKLDDSGLEFEISIFDIPNTVSDTIKINIYRIIQECINNILKHA
                     NATKVYITLEADEEYIHGLIEDNGQGFDIHAAKKKGIGIDNICTRIH"
BASE COUNT          497 a          364 c          364 g          637 t
ORIGIN      
        1 aaatgaatgc gggtgcagat gttatcaata ccgatacctt tctttttggc tgcatgaata
       61 tcaaaaccct gcccgttgtc ctcgattaag ccgtggatgt attcctcgtc agcttcgagc
      121 gtgatgtaca cttttgtggc atttgcgtgt tttagaatat tgttgataca ttcctgaatt
      181 atccggtaaa tgttgatttt tatcgtatcg cttaccgtat tcggaatatc gaaaatggat
      241 atttcaaatt ctaaaccgct gtcatccagc ttgtttacaa gatcttcaac agccgttttg
      301 aatcctttgg taataataaa atttgtcacc agttcgtgtg aaacttcgcg ggtttcgcgg
      361 atagaagttt ccactaaatc cagcgttttg tgatatactt catttacttt cgggtcatcg
      421 ggattcaggt gagtttgcat aaactgcata ttcattttaa tagcactaag caattgaccg
      481 agtccatcgt gaagcgaagc tccgatgcgg gttttttcat tttcttcggt ttcaaatatt
      541 gattgtgtca gtttttgttg ctgttcgagt tcgatggatt taatttttga ttgctgaatg
      601 tattggagtg agcgataaat cacaacacct gttagtatta ttccaatgag cgaaatgatt
      661 gcaaaccaga taatcagatt cttccggtta atctcggatt cctggttttt tattgtgatt
      721 tctttttttg aaagttcgta ttttgcttcc atctccgaaa gtttttctgc agaaatggca
      781 atattggttg agtcttttat tttttcaagc agcagcaatg tttctgtgct tttcttgtaa
      841 tttcctgaac cataataatt tttgaaaagc gcatcaagaa ctattggctt tttggatgca
      901 aggccgtttt ctccggtcat ttcccatgct ttttgtgcca gattaatacc ttctgcataa
      961 ttccctgttt cggcatacaa acccgaaagt tgagccaaat ccgaaacgac atagaaagga
     1021 tcgccgatat ggaggcgaag ctgcatggat ttcttcaccg catcgatggc tttttgattc
     1081 aatccgcggg ctgagtaaat attggctaaa atattatagg aattggcttc attcagcaga
     1141 ttattgaatt ttacggaaag ctcaatagct ttctgaatca acacgttagc tgaatctaaa
     1201 ttattttgca tgccataaat tacacttaga tttatgtagc tcggaatttt aatctcttcc
     1261 aatctggaag acacgggcaa attcagtacg ctcaaaagcc attttttagc ctcatcagtg
     1321 cgttgaattt ccatatttgc ccaccccaat ccgtgcatag ccgacaaata cgaaagtgtg
     1381 tcactatgaa tatcggattc atccagtaaa gaaaagtatc tgctgattgc ctcctggttt
     1441 tgcccttttt ttatcagaac agagctgatt aacaaacggt ttttccggtc aagggaaaca
     1501 tcccgaatgc tgcttttctc cagttgtcta caaatgttta gggcgctatc cagctggctg
     1561 gtacggtaaa aataagttgc cagccagaat ttactctgca attctgaatc ttcattctgc
     1621 aaacgccgtg aaagcaacag cgcttctgaa gcatatttct tcaacgtatc atacgggaag
     1681 gttttatatt ctccgcaaaa gtcaagtaat gccttcaaac ccgtttcgtt tgatgctgct
     1741 ttcctgtatt gttgcaaata tgcttcagct ttcggtgtat aggccgatat atttgcgtag
     1801 agaaacaaga gcaaaagggt gaatcgtaat cttaacttca tttttcagaa aataatgtga
     1861 ac
//
LOCUS       DAORKP010000262         1858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24157_length_1858_cov_10.478092, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000262 DAORKP010000000
VERSION     DAORKP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24157_length_1858_cov_10.478092"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLN75_09640"
     CDS             <1..298
                     /locus_tag="PLN75_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRG04416.1"
                     /translation="ADVLAAAEVGRERQKKFLMYAQRMIRENYIFNISQPGLNYMADY
                     EAGFSGNFSPYINERNVEDLVQEFSLAERHIEQNVNAKMVFFDLVLKVIMLLKR"
     gene            428..1594
                     /gene="ricT"
                     /locus_tag="PLN75_09645"
     CDS             428..1594
                     /gene="ricT"
                     /locus_tag="PLN75_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRG04417.1"
                     /translation="MEYRLNNGGCCMNKKGCSKASGKKLSVYDWLCDLPETIKETDLV
                     EVQFKNTRKGYYLNNSQLELQKGDKVAVESSPGHDIGEVTLVGKLVLLQMKKNNIDTS
                     KYEIRKVYRKVREMDMEKYNEAKSREQETMIKARQIAESLHLSMKIGDVEFQGDGNKA
                     IFYYIADERVDFRQLIKVFAETFRVRIEMKQIGARQEAGRIGGIGPCGRELCCSTWIT
                     SFNTVSTSAARYQDLSLNPQKLAGQCGKLKCCMNYELDSYMDAQRNLPSREIELETKN
                     GTFYHFKTDIFKGLITYSSDRNMASNLVTISSERAKEIIAMNKNGKKPDELLTQTVEK
                     KKSTEYENVVGQDSLTRFDTEKKQGKNPRKKQLKPRFNNDKSKPNHNPKNNEGK"
     gene            1581..>1858
                     /locus_tag="PLN75_09650"
     CDS             1581..>1858
                     /locus_tag="PLN75_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04418.1"
                     /translation="MKANKILAVFLLIGILASCNRNEVYFDYRTVDAGGWSKDSLLVY
                     NIDVKDSVSKFDIYVHVRHYGTYPYQNLWMFIDYKDMKDSIPKHDTIE"
BASE COUNT          646 a          372 c          412 g          428 t
ORIGIN      
        1 ggccgatgtg ctggccgctg cagaggtggg gcgcgaaagg cagaaaaaat tccttatgta
       61 tgctcagcgc atgattcgtg aaaattacat ctttaatatc agtcagcccg gtctgaacta
      121 catggccgat tatgaagccg gcttttcagg caatttctca ccatatatta acgagagaaa
      181 cgtggaagat ttggtacagg aattttcgct ggccgaacga cacatcgaac aaaatgtaaa
      241 tgcaaagatg gttttctttg acctggtgct caaggttatc atgctgctga aaagataatg
      301 aaaataatag caaaggtaga aatccgatac aggaaatgac ggtttttatt gaagaaatca
      361 acataaatat aaaaacaaaa tgcgaccagg aatatcaatc ttaggcgaac aaacaatata
      421 agaatacatg gaatacagac taaataacgg gggctgctgc atgaataaaa aaggctgtag
      481 caaagcttcc ggcaaaaaat taagtgttta cgactggctc tgcgatctgc ccgaaacgat
      541 aaaagaaaca gacctggtag aagtgcagtt taaaaatacc cgaaaaggtt attacctcaa
      601 taactcacag ctcgaactgc agaaaggcga taaagttgcg gtggagtcat ctcccggaca
      661 cgatattggc gaagtgaccc tcgtagggaa acttgtactt cttcagatga agaaaaacaa
      721 catcgatact tcgaagtacg aaatcagaaa agtgtaccgc aaagtgcgtg aaatggatat
      781 ggaaaaatac aacgaagcca aatccagaga acaggaaaca atgatcaaag cacgtcagat
      841 tgccgaaagc cttcatctga gcatgaaaat tggcgacgtg gaatttcagg gcgacggcaa
      901 caaggccatt ttttattaca tagccgacga acgggtggac ttcaggcagt taatcaaggt
      961 ttttgccgaa actttcaggg tgcgcatcga aatgaaacaa attggtgctc gtcaggaagc
     1021 cggaagaatt gggggaatag gaccgtgcgg acgtgaactt tgctgctcca cctggattac
     1081 cagctttaat acagtatcca ccagcgcagc ccgttatcag gacctgtcgc tcaatccgca
     1141 gaaacttgcc ggacaatgcg gcaaactgaa gtgttgcatg aactacgagc tcgacagcta
     1201 catggatgcg cagcgcaatc tgccttcacg tgaaatagaa cttgaaacca aaaacggtac
     1261 tttttatcat ttcaaaacgg atattttcaa agggctcatt acctattctt ccgacagaaa
     1321 tatggcatcc aatctggtta ctatctcttc cgaaagagca aaagaaatta tagccatgaa
     1381 caaaaacggc aaaaaaccgg atgaacttct tacgcagact gtcgaaaaaa agaaatcaac
     1441 cgaatatgaa aacgtggtgg ggcaggatag tcttacacga tttgatactg aaaaaaaaca
     1501 gggtaaaaat ccccgaaaaa aacaactgaa accccgattc aacaacgata aatccaaacc
     1561 gaaccataat ccgaaaaaca atgaaggcaa ataaaattct tgctgttttt ttgttgattg
     1621 gtatcctcgc atcctgcaat cgtaacgaag tctacttcga ttacaggacg gtagatgctg
     1681 gcggctggag taaggacagt ctgctggttt acaacatcga tgtaaaggat tcggtttcaa
     1741 aattcgacat ttacgtccac gtaaggcatt acggtactta tccttatcag aatttatgga
     1801 tgtttataga ttacaaggat atgaaagaca gtatcccgaa gcacgatacc attgaatg
//
LOCUS       DAORKP010000263         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24273_length_1853_cov_7.828142, whole genome shotgun sequence.
ACCESSION   DAORKP010000263 DAORKP010000000
VERSION     DAORKP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24273_length_1853_cov_7.828142"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLN75_09655"
     CDS             <1..912
                     /locus_tag="PLN75_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HRG04419.1"
                     /translation="SSRGIYLFPVTESISSIVRDGRGGDSKLAHVSSYELYAVDFGSS
                     ADKVFKMQDQHPFVAGEFVWTGWDHLGEPTPYYSARSSYCGMIDLAGFKKDRFYLYQS
                     RWRPELPMVHILPHWNWTDRKGEITPVHVFTSGDEVELFLNGKSQGRKKKQSLEYRLR
                     WDEVTYEPGELRAVAYKNGKKWAENSVKTTGKAVGLTAEADRKTIKSDGYDLAFITVK
                     IVDKDGLTVPHSNNKIRFSIEGPGEIVATDNGDQTDFTPFNSRERNAFNGLCLVIVKG
                     IKGQKGNINIYTEADGLKAGKVVLTGK"
     gene            925..>1853
                     /locus_tag="PLN75_09660"
     CDS             925..>1853
                     /locus_tag="PLN75_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04420.1"
                     /translation="MKIKKIHNLRVILILLIVFPIAGFSAGKIPLVYSVENRGEKFAK
                     PAMPDLSQLPVIEPLTDPFSWSNGKGRSTKFRDWSKRRNEIGAEIQHYEIGIKPARPK
                     NITAEYENDTLTVKITVNGETLTLTSNIILPEGKGPFPAVIGIGRGSGSLPPDLFSTR
                     NIVQIPFNFAQVMAWQQKRGSEPINKLYPELVHMGAYSAWSWGVSRLIDGLELVAEKL
                     PVDLNYIGITGCSFAGKMALYAGAFDERIALTIAQESGGGGAAAWRVSETLGNVETLG
                     KTSHVWFMEDLFRFSKDVSKLPFDHHELMAMVAP"
BASE COUNT          549 a          383 c          473 g          448 t
ORIGIN      
        1 agcagtagag gaatctatct tttcccggtt acagaaagca tcagttccat cgtgcgagac
       61 ggcaggggag gtgattccaa gctggctcac gtcagctcat acgagcttta cgctgtggat
      121 ttcggttctt cggcagataa agttttcaaa atgcaggatc agcatccgtt tgttgccggc
      181 gaattcgtct ggactggctg ggatcacctc ggcgaaccta cgccttacta ttcagccaga
      241 agctcgtatt gcggtatgat cgatttggcg ggtttcaaga aagatcgttt ttatctctac
      301 caatcacgct ggcgtcctga attgccgatg gttcacatcc ttcctcattg gaattggact
      361 gaccgaaaag gcgaaatcac ccccgtccac gtattcactt caggcgatga agtggaatta
      421 tttctgaatg gaaaatcaca gggacgaaag aaaaaacagt ccctcgaata ccgactccgc
      481 tgggatgaag tgacctatga acccggtgaa ctgagagctg tggcgtataa aaacgggaag
      541 aaatgggctg aaaacagtgt gaaaactacc ggaaaagctg ttggattgac tgccgaagca
      601 gaccgaaaaa cgataaaatc agacggttac gacctggctt ttatcacagt gaaaatcgtg
      661 gataaagatg gtttaacggt cccgcactcg aacaacaaaa tccgtttcag catcgaaggt
      721 ccgggtgaaa ttgtcgctac cgacaacggg gatcaaaccg attttactcc gtttaattca
      781 agggagagaa atgctttcaa cggtctttgc cttgtgattg tgaaagggat aaaagggcag
      841 aaaggcaata tcaatattta tacagaagca gatggattaa aagccggaaa agtagtgttg
      901 acgggtaaat aacgcatgaa ttttatgaaa ataaaaaaaa tacataatct ccgggtaata
      961 ctgattctgc tgatcgtttt tccaatcgca ggtttttcag ccgggaaaat cccgcttgta
     1021 tacagcgtgg aaaacagagg agagaaattt gcaaaaccgg cgatgcctga tcttagccaa
     1081 cttcctgtta tcgagccgtt gaccgatccc ttttcctggt caaacggaaa aggcagatcc
     1141 acaaaattca gggattggag caaacgccgg aacgaaatag gggcagaaat tcagcattat
     1201 gaaattggca tcaaaccagc gcgaccaaaa aacatcacgg ccgaatacga aaatgataca
     1261 cttacggtaa aaattacggt gaatggagag acattaacct taacttcaaa tattatttta
     1321 cccgaaggaa aaggtccttt tcctgctgta atcggtattg ggagaggttc gggaagtctg
     1381 ccgcctgatt tattttccac gcgaaacatt gttcagatac cgttcaattt tgcacaggtt
     1441 atggcctggc agcaaaaacg gggaagcgag ccgattaaca aactttatcc ggaattggtg
     1501 cacatgggtg cttacagcgc ctggtcgtgg ggtgtgagcc ggctgattga cggtttggaa
     1561 ctggtagcag aaaaactgcc ggttgacctg aattacatcg ggattacagg ctgttcattt
     1621 gcagggaaaa tggcacttta tgccggagca tttgatgaac ggatagcact caccatagcc
     1681 caggaatcgg gtggaggagg cgcagcagcc tggcgcgtgt ccgaaacact gggaaatgtg
     1741 gaaacgctgg gcaaaaccag tcatgtgtgg tttatggaag atttattcag gttttcaaaa
     1801 gatgtatcga aattgccgtt tgatcatcac gaactgatgg caatggtggc tcc
//
LOCUS       DAORKP010000264         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24485_length_1844_cov_8.483510, whole genome shotgun sequence.
ACCESSION   DAORKP010000264 DAORKP010000000
VERSION     DAORKP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24485_length_1844_cov_8.483510"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLN75_09665"
     CDS             <1..252
                     /locus_tag="PLN75_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRG04421.1"
                     /translation="APIIAEAPICIECKVREIIPLGSHDMFIAEVVNVQADARYIDKK
                     TDKFDMQKAKLIAYSHGHYYKLGEEIGRFGWTVRKKPAP"
     gene            377..1657
                     /gene="glyA"
                     /locus_tag="PLN75_09670"
     CDS             377..1657
                     /gene="glyA"
                     /locus_tag="PLN75_09670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRG04422.1"
                     /translation="MTRDTQIFDIIRREKERQMKGIELIASENFVSNQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCEVVDESEQLAIDRIKEIFGAEWANVQPHSGAQANMAVFLACL
                     NPGDKFLGLNLAHGGHLSHGSPVNSSGILYKALEYNVKEETGRVDYDQMEEVALRERP
                     KLIIGGGSAYSREWDYKRMREIADEIGAIFMVDMAHPAGLIAAGLLENPVKHAHIVTS
                     TTHKTLRGPRGGIILLGKDFPNPWGKTTPKGEVKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVSFGECLQPEYREYQIQVKKNAAALAEALVKRGFKIISGGTDNHSMLVDLRSKYP
                     DLTGKVAEKILVEADITCNKNMVPFDSRSAFQTSGIRLGTPAITTRGAKEPLMEEIAE
                     MIETVLSNVENPDVIKSVKDRVNKTMSNYPLFAY"
BASE COUNT          564 a          408 c          438 g          434 t
ORIGIN      
        1 gcaccgataa tagctgaagc gcccatttgt atcgaatgta aagtcaggga aatcattccg
       61 ttgggttcgc atgatatgtt tattgctgaa gtggtaaatg tgcaggccga cgcccgctat
      121 atcgataaaa aaacggataa atttgatatg caaaaagcca aacttatcgc ttattcgcac
      181 ggacattatt acaagttagg agaagaaatc ggacggtttg gatggacggt gaggaagaaa
      241 ccagcccctt aaacctgcct ggtttatccc gtcctgtcag agcggcaggc aggtcacccg
      301 aaaggagact ttctctgacg aaaatatcca ttaaaaaaca caattaatag aaattaaaaa
      361 tatataccaa agaacaatga cacgagacac acaaattttt gacattatca gacgtgaaaa
      421 agagcgtcag atgaaaggaa tcgaacttat tgcatccgaa aacttcgtta gcaatcaggt
      481 gctggaagcg atgggttcag tattaaccaa taaatatgcc gaaggttatc ccggtaagcg
      541 ctattacggc ggatgcgaag tggtggacga gagcgaacaa cttgccattg accgtattaa
      601 ggaaattttc ggagccgaat gggctaacgt acagccgcac tccggcgcac aagccaacat
      661 ggctgtattc cttgcctgcc tgaatccggg agataaattt ctgggattaa acctggcaca
      721 cggtggccat ctttcacacg gttcgcctgt gaacagttcg gggattttgt ataaagcgct
      781 ggaatacaac gtaaaagaag aaacaggacg tgtggattac gaccaaatgg aagaagtggc
      841 actgcgagaa cgcccgaaac tgattatcgg cggcggatca gcctattccc gcgaatggga
      901 ttacaaacgg atgcgtgaaa ttgcagatga aattggcgca atttttatgg ttgacatggc
      961 tcatccggcc ggattaattg ctgccggatt gcttgaaaat ccggtgaaac atgcgcatat
     1021 cgttacttcc actactcaca aaacgcttcg cggaccacgt ggagggatta ttttacttgg
     1081 aaaggatttt ccgaatccat ggggcaaaac cactccgaaa ggagaagtaa aaatgatgtc
     1141 agccctactc gattcagccg tttttccggg aatccagggc ggtccgctgg aacacgtcat
     1201 tgctgccaaa gccgtttctt tcggcgaatg tctgcagcca gagtacaggg aatatcagat
     1261 tcaggtgaag aaaaatgcgg ctgcattggc tgaagcattg gtaaaacgcg gtttcaaaat
     1321 catctccggc ggaacagaca atcactccat gctggtggat ttgcgctcca aatatcccga
     1381 tctgaccgga aaagtggctg aaaaaatact ggtggaagcc gatattacct gcaacaaaaa
     1441 catggttccg ttcgacagcc gttctgcatt ccagacttcg ggaattcgcc tgggaacacc
     1501 tgctattact acccgcggcg ccaaagaacc gctgatggaa gaaattgctg aaatgattga
     1561 aaccgtactt tcgaatgtag aaaatccgga tgtaatcaaa tcggtaaaag accgggtgaa
     1621 caaaacaatg agcaactatc cgttgtttgc ttattaaagc cccaccaacc tccccaccag
     1681 ctgacggaat ggcttacaac tctcttatga ataaaaaaaa taatataaat tcaagccttg
     1741 aaatcccccc tttggggggc ggaggggctc ttttcaccct ttccgaacac acagattctg
     1801 ccatttttta tggcgtgaat aattccaata ttcagttact gagc
//
LOCUS       DAORKP010000265         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24626_length_1838_cov_7.291643, whole genome shotgun sequence.
ACCESSION   DAORKP010000265 DAORKP010000000
VERSION     DAORKP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24626_length_1838_cov_7.291643"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..>1838)
                     /locus_tag="PLN75_09675"
     CDS             complement(151..>1838)
                     /locus_tag="PLN75_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRG04423.1"
                     /translation="CTSSCKPEEVVQPELIVAKEEIPFTKNAGSILLAIKTNVKWRAS
                     SNESWCTLSPASGEAGSRQIAVWVTENTSVAVREAIITITAGSLSKQVKVIQAQKVVF
                     TISQKEFNVPGQGGEITVTIQASQDFTTAIQAAWITQKSISADKKTQIFTVAENDLDS
                     LREGTITFTSGSMTEIVSVRQTTKDFSIPPDKTGMDSDAKVLAKKMHLGWNLGNTLEA
                     IGGETAWGNPKASKTLIDLVKASGFNAVRIPCAWDQYLENQTTYKIKDLWLARVKEVV
                     DYCIENDMYAILNIHWDGGWLENNPTPDKQAEVNKKQKAIWEQIALYFRNYDERLLFA
                     GTNEVHTTSGNPTQANFDVQMSYNQTFVDAVRFTGGKNAYRNLIIQAYNTNIDQAVAY
                     LKISTDYVPDRLMVEVHHYDPWNFCGLEKDETWGKYAALWGQPYEQYAIGVLAGRAAT
                     WGKEDYLKTQFNKIKTSFVDKGYPVILGEYGVIRRTTYSGDALMHHLDSRAYYLRYVT
                     EQAKNYGLIPFYWDNGSTGNLAFGIIDRKTNRIADEKALNALIEGSVAGTYPY"
BASE COUNT          479 a          422 c          371 g          566 t
ORIGIN      
        1 ataatttaaa aaattctact aaaataagaa attttaaaat atccaggatc aacaattaaa
       61 aacagacaga gaaaacttca ttctctatct gttggatttt taataatttt ttataggtga
      121 aatggaaaaa tgagataaaa atacaactaa ttaataaggg taagttcctg ccacactacc
      181 ctcaatcagt gcatttagtg ccttctcatc agcaattcga ttggtttttc ggtcgattat
      241 accaaaagcc aggtttccgg tactgccgtt gtcccagtaa aagggaatca gtccatagtt
      301 tttggcctgt tcggtcacat acctgaggta ataagcgcgg gagtcgaggt gatgcatcag
      361 tgcatcgccg gaatacgtcg tgcggcgtat cacaccgtat tctcccagta tcaccggata
      421 gcctttgtcc acaaaactgg tttttatttt attgaactgt gttttcaaat aatcttcctt
      481 gccccatgta gcagctcttc cggccagaac acctatagca tattgttcat aaggctgacc
      541 ccaaagcgct gcatattttc cccaggtttc atccttttcc agaccacaga aattccacgg
      601 gtcgtaatga tgcacttcca ccatgagtct atcggggacg taatcggtcg aaattttaag
      661 gtatgccacc gcctgatcaa tgttggtgtt gtaagcctga ataatcaaat tccgataggc
      721 atttttccct ccggtgaaac gtactgcgtc cacgaaagtt tgattgtagg acatttgaac
      781 gtcaaaattt gcttgagttg gattgccgga agtggtgtgc acttcattcg tacctgcaaa
      841 aagcagtcgc tcatcgtaat ttctgaaata aagtgcaatc tgctcccaaa ttgctttttg
      901 ttttttgttg acttcagcct gtttgtcggg agttgggtta ttctcgagcc aaccaccgtc
      961 ccagtggatg tttaagatgg cgtacatgtc attttcaata cagtaatcaa ccacttcctt
     1021 cacccgcgcc agccataaat ccttgatttt gtaggttgtc tgattttcca aatactgatc
     1081 ccaggcacac ggtattctca ccgcattgaa gcctgatgct tttaccagat cgatgagcgt
     1141 cttcgatgct ttgggattcc cccaggctgt ttcgcctcca atggcttcca gcgtatttcc
     1201 caggttccat cccaaatgca ttttctttgc aagaactttt gcatcactgt ccatacccgt
     1261 tttatcgggc gggatggaga aatcttttgt tgtctggcgt acggatacaa tttcagtcat
     1321 tgagccggaa gtgaatgtga ttgtcccttc gcggagagaa tccaggtcat tttccgccac
     1381 tgtaaaaatc tgagtttttt tatcggcaga aatagatttc tgagtaatcc aggcagcctg
     1441 aattgcagtt gtaaaatcct gtgatgcctg aatagttaca gtaatttccc ctccctgtcc
     1501 gggcacattg aattctttct gagaaatggt aaaaacaact ttttgagcct gaataacctt
     1561 tacctgcttc gaaagcgaac ctgctgtaat cgtaataata gcttcacgca cagctacaga
     1621 agtgttttca gtaacccaaa ccgcaatttg cctgctgcct gcctcgcccg atgcgggtga
     1681 aagcgtacac cacgactcgt tactgctcgc tctccatttc acattcgttt taatggcaag
     1741 aaggatacta ccggcatttt ttgtaaatgg tatctcttct ttggctacta tcaattccgg
     1801 ctgaactact tcttcgggtt tacaggatga agtgcaga
//
LOCUS       DAORKP010000266         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24643_length_1837_cov_9.975309, whole genome shotgun sequence.
ACCESSION   DAORKP010000266 DAORKP010000000
VERSION     DAORKP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24643_length_1837_cov_9.975309"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLN75_09680"
     CDS             <1..705
                     /locus_tag="PLN75_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HRG04424.1"
                     /translation="NLAYGAAGLSIVADSLSAIKHAKVTVVRDENGLSKDFKIEGEFP
                     YYGNDDDRVDVFAREIPIYFNSLLKELPTYKNAEPTLSILTITSNVVYGNKTGATPDG
                     RAKGVPFAPGANPMHGRDSHGALASLSSVSKLDYEHSMDGISNTFSIVPKTLGPTDEQ
                     RRENMVSIMDGYFSKNAHHLNVNVLNREMLEDAMEHPEEYPQLTIRVSGYAVNFVRLT
                     RAQQQEVIARSFHEAM"
     gene            790..1518
                     /gene="pflA"
                     /locus_tag="PLN75_09685"
     CDS             790..1518
                     /gene="pflA"
                     /locus_tag="PLN75_09685"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRG04425.1"
                     /translation="MIKLHSVESLGTFDGPGVRMVLFTQGCNFKCKYCANPDTIDLNR
                     EAKLFSYEELMKLARSQRPFFGKRGGITVSGGEPLLQAKALIPFFKMLKEEGFNTCID
                     TNGSILNESVKELMQYTDLVLLDVKEFNEEAHRQLIGASNKATLRMAEYLHGINQPVW
                     LRYVLVPGHSDKSEHLHDLGRFLQPMQNIEKLEIQPYHKLGAHKYEALGWNYELEGVE
                     QNTTEQLRVAKEIFSLYVKEVIIN"
     gene            1522..>1837
                     /locus_tag="PLN75_09690"
     CDS             1522..>1837
                     /locus_tag="PLN75_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRG04426.1"
                     /translation="MSYYTPKQIVETVSNVAAAKASTSTPKTLVLAFLAGAYIAIGGL
                     LAVIIGGGIPGIAAENPGIQKFLMGAAFPIGLMLCTLAGADLFTGNTAYFIPPVMDKK
                     LRV"
BASE COUNT          513 a          398 c          439 g          487 t
ORIGIN      
        1 aaccttgcgt atggtgctgc cggtctgagc attgtggccg actcactttc ggccatcaaa
       61 catgccaaag taactgtagt gcgcgacgaa aacggtttgt cgaaagactt taaaattgaa
      121 ggtgaattcc catattatgg aaatgatgac gaccgcgtgg atgtatttgc ccgcgaaatt
      181 ccgatttatt tcaactcatt gctcaaggaa cttccaacat ataaaaatgc tgagccaacg
      241 ctgagtattc ttaccattac ttcgaatgtg gtttatggta acaaaacagg tgctacaccc
      301 gatggacgcg ctaaaggtgt tccgtttgcg ccgggagcaa acccaatgca cggacgcgat
      361 tcgcacggtg cgcttgcttc gctgagctcg gtgtcgaaac tcgactacga acactcgatg
      421 gacggaattt cgaatacatt ctctattgtg ccaaaaacct taggaccaac cgacgaacag
      481 cgtcgtgaga atatggtgag cattatggat ggatatttct cgaaaaatgc ccatcacctg
      541 aatgtgaacg tgctgaaccg cgaaatgctc gaagatgcca tggagcatcc tgaagaatat
      601 ccacagctta ctatccgcgt gtcgggttat gcggtgaatt ttgttcgtct tacacgtgca
      661 cagcaacagg aagtgattgc acgaagcttc cacgaagcta tgtaatgacg catttaccaa
      721 taaaactata aaaattatca gaagtaaccg gctgcatttt tcagctggtt acttctttgc
      781 ttatcacaaa tgattaaact gcattcggtt gaatcgctcg gtaccttcga tgggccgggt
      841 gtacgtatgg tactttttac acagggctgc aatttcaaat gtaagtattg tgccaatccc
      901 gatactatcg atttgaatag agaagctaag ctgttcagct acgaagaatt aatgaaactg
      961 gcccgcagtc agcgtccttt tttcggaaaa cgtggtggca ttactgtgtc gggcggcgag
     1021 cctcttttgc aggccaaagc cctgattcct ttttttaaaa tgctgaaaga agaaggtttt
     1081 aatacctgta ttgataccaa cggaagtatt ctgaatgagt cggtaaaaga gctgatgcaa
     1141 tataccgacc ttgtattgct tgacgtaaaa gaatttaatg aagaagcgca ccgacaactt
     1201 atcggagctt cgaacaaggc tacactgcga atggcagaat acctgcacgg cataaatcag
     1261 cccgtttggc tgcgttatgt acttgtgccg ggacattcgg acaagtcgga gcatttacac
     1321 gatctgggac gatttctgca acctatgcag aatatcgaaa agctcgaaat tcagccctac
     1381 cataaacttg gtgcgcacaa atatgaggca ttgggctgga attacgaact cgaaggtgtg
     1441 gagcaaaaca ctaccgagca acttcgtgtg gccaaagaga tttttagttt gtatgttaaa
     1501 gaagtgatta tcaattaagg tatgagttat tacacaccca aacaaattgt ggaaacggtg
     1561 agcaatgtgg ctgcagcgaa agcttccact tctacgccca aaacgcttgt gctggctttt
     1621 ctggccggtg cttatattgc catcggaggt ttgttggcag tgatcatcgg aggcggaatt
     1681 ccgggcattg cagctgaaaa ccccggcata cagaaatttc tgatgggcgc tgcgttccca
     1741 atcggactta tgctttgtac gctggcaggc gccgatttat ttaccggaaa tacggcttat
     1801 tttattcctc cggtaatgga taaaaagctt cgcgtaa
//
LOCUS       DAORKP010000267         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_24848_length_1828_cov_6.260575, whole genome shotgun sequence.
ACCESSION   DAORKP010000267 DAORKP010000000
VERSION     DAORKP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24848_length_1828_cov_6.260575"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLN75_09695"
     CDS             <1..470
                     /locus_tag="PLN75_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRG04427.1"
                     /translation="EIVDNVSPLGFILGDEGSGAVLSKTFMSDCLKNQIPAELRKAFM
                     DKYELTPAVVLENVYKKPFPNRYLATFTPFLSENISEPAIRDVVYNGFVAFFKRNVMQ
                     YEFQTMKTSFVGSVAFHFEELLLKAAADCGVTVGTVVKSPMQGLIKYHASQQK"
     gene            529..1353
                     /gene="murQ"
                     /locus_tag="PLN75_09700"
     CDS             529..1353
                     /gene="murQ"
                     /locus_tag="PLN75_09700"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRG04428.1"
                     /translation="MAFQKITEQNSLHDNLDKKSVHDLLIEMNEEDMKVPLAVSRALP
                     QIEELVEKIVERMKRGGRIFYLGAGTSGRLGVLDASEIPPTFGMPASLVVGLIAGGET
                     ALRNPVENAEDDIEKGWQELKERNVNENDTVIGIAASGTTPYVIGALRNARANGILTA
                     SVSCNPDSPIAQEAEIKIEAIVGPEFVTGSTRLKSGTAQKLILNMITTITMIKLGRVK
                     GNKMVNMQLSNAKLVDRGARMIVEELGIDYEHAKNILLIQGSVKKAVDAFKLQQNK"
     gene            1376..>1828
                     /locus_tag="PLN75_09705"
     CDS             1376..>1828
                     /locus_tag="PLN75_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HRG04429.1"
                     /translation="MKQSFLLFIFIAFLLSFSSGTKAQHSRTDVAFDKKDSLIVENIL
                     GALKNKTTLSTGDLIIAGGKMLEGTPYVANTLDGGTAEKLIINLRALDCTTFAENCLA
                     LARTAQSATPDFATFVNELRSIRYRNKKLTDYSSRLHYFSDWIYNNEQK"
BASE COUNT          566 a          322 c          419 g          521 t
ORIGIN      
        1 ccgaaatagt ggacaatgtt tctcctttgg gtttcattct tggcgatgaa ggaagtggtg
       61 cagtgttgtc caaaactttt atgtccgact gcttgaaaaa tcagattcct gcggaattgc
      121 gtaaagcttt tatggataaa tacgaactca ctccggcagt agtactcgag aatgtttata
      181 aaaaaccatt tccgaatcga tatcttgcta cctttacacc tttcctgagt gagaatattt
      241 cagagcctgc aattcgcgat gttgtgtaca acggattcgt cgcttttttc aaacgaaacg
      301 ttatgcagta cgagtttcag acaatgaaaa cttcgtttgt aggttcagtg gcatttcatt
      361 ttgaagaact actgcttaaa gcggctgccg attgcggagt tactgttggt actgttgtaa
      421 aaagtccaat gcagggtttg ataaaatatc atgccagtca acaaaaatag tatttttggt
      481 ttttcggagc cttcgtatta ataaataaaa taatcaaaat cagaacatat ggcatttcaa
      541 aaaattacag aacaaaactc gctacatgac aatttggata aaaaatcggt tcacgatttg
      601 ttgatcgaaa tgaatgaaga agatatgaaa gttcccttgg cagtttcaag agccctacca
      661 caaatagaag aactagttga aaaaattgtg gagagaatga agcgtggagg tcggattttt
      721 tatttgggag ccggtaccag tgggcgttta ggtgtgcttg atgcatccga aattcctccc
      781 acctttggta tgccagcgag ccttgtggta ggtttaattg ccggaggcga aactgctttg
      841 cgtaatccgg tagaaaatgc cgaagatgat atcgaaaaag gctggcagga actgaaagaa
      901 agaaatgtga atgagaatga tacggttatc ggaattgctg cttcgggtac aactccgtat
      961 gtgattggtg ctttacgcaa tgcacgtgcc aatggtatac taactgcttc agtttcatgt
     1021 aatccggatt cacctattgc tcaggaggct gagattaaaa tcgaagctat tgtgggaccg
     1081 gagtttgtga ctggaagtac ccgccttaaa tccggaactg ctcagaagtt gatattgaat
     1141 atgataacca ccattacgat gattaagctc ggacgcgtaa aaggaaataa aatggtgaat
     1201 atgcagctgt cgaatgcaaa gcttgtggac cgtggcgccc gaatgatagt ggaagagctg
     1261 gggattgatt acgaacatgc taaaaatatc ctgcttattc agggctcggt aaagaaagct
     1321 gtggatgcat ttaagctgca gcaaaataaa taaaaattaa aataatactg gttcaatgaa
     1381 acaatcattc ctgctgttta ttttcatcgc atttctgttg agttttagtt caggaactaa
     1441 ggctcagcat agcaggactg atgttgcttt tgacaaaaaa gatagcctga ttgtcgaaaa
     1501 tattctcgga gctctcaaga ataaaaccac tttatcgaca ggtgatctga tcattgcagg
     1561 cggaaaaatg ctggagggaa cgccctacgt tgcaaatact ttagatggtg gaacagcaga
     1621 aaagctgatt attaacctgc gagcgctcga ttgtactact tttgccgaaa attgtttagc
     1681 cctggcccgt acagctcagt cagcaactcc ggatttcgct actttcgtga atgaattaag
     1741 gagtataagg tataggaata aaaaattaac tgattacagt tcccgtttgc attatttcag
     1801 cgactggatt tataacaatg agcaaaaa
//
LOCUS       DAORKP010000268         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_25142_length_1816_cov_8.239637, whole genome shotgun sequence.
ACCESSION   DAORKP010000268 DAORKP010000000
VERSION     DAORKP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25142_length_1816_cov_8.239637"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLN75_09710"
     CDS             <1..953
                     /locus_tag="PLN75_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HRG04430.1"
                     /translation="PELQITQEYLGFSNHIAYLAPMWKECLDSDTYCKGEGSTVAKTT
                     DGSIYNQKITAIAGVANIGEDLNWCGNHFAQANWYAFGRLAWNHELTSEQIADEWLKM
                     TFTGDRNFIEPVKEMMLSSRESVVKYMMPLGLHHLFAWGHHYGPEPWCEVPGARPDWL
                     PKYYHNANDTGIGFDRTTSGSNAVSQYNSPLREQFNDIGTCPENLILWFHHVPWTYGM
                     KSGNSLWDELCYTYDSGVQEVRDYQKTWDKLEKYVDAERFRHVQSRLKIQSRDAVWWK
                     DACLLYFKQFSHMPIPYDIERPVWELEELMKIKLDMTHHN"
     gene            1050..>1816
                     /locus_tag="PLN75_09715"
     CDS             1050..>1816
                     /locus_tag="PLN75_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRG04431.1"
                     /translation="MKTNVSKFSFFLLIILLTGCASGKKTNEWISLFNGKDLTGWTVK
                     IKGYKAGENFGNTFRFEDGILKVRYDAYETFDDRFGHIFYKDKFSHYIVRFEYRILGE
                     QCPGGPEWGYKNTGILIHGQTPESMDLDQNFPTSIEVQLLGGDSVGERSTGNVCTPGT
                     NIVINNQLILEHCTNSSGSTIRTDEWVRVEVEVHGDSIIRHFINDEQVIAYEKPQLDD
                     RDATFSKLLPLNGGKIQLTEGTICLQSESHPCDFRNV"
BASE COUNT          526 a          385 c          446 g          459 t
ORIGIN      
        1 tgcccgaact gcagattacc caggaatacc tcggtttctc taaccacatt gcctatctgg
       61 ctccgatgtg gaaagaatgc ctggatagcg atacctattg caaaggtgaa ggctcaacgg
      121 tggcaaaaac aactgatggc tctatttaca atcaaaagat tacggctatt gcaggcgttg
      181 ccaatatcgg cgaagacctc aactggtgcg gaaatcactt tgcgcaggcc aactggtatg
      241 cattcggacg attggcgtgg aatcacgaac ttacatcaga acagattgcc gacgagtggt
      301 tgaaaatgac atttaccggc gatagaaatt tcattgaacc ggtgaaagaa atgatgcttt
      361 cctcgcgcga gtcggtcgta aaatacatga tgccgctggg tttacatcat ctttttgcct
      421 ggggacatca ttacggtccg gaaccctggt gcgaagtgcc cggagcacgg cccgactggc
      481 tgccaaagta ttatcacaac gccaacgata caggaattgg ctttgaccgt actacctcag
      541 gcagcaatgc cgtaagtcag tacaattcgc cgcttcgtga gcagttcaac gacatcggaa
      601 cctgcccgga gaatctgatt ctttggtttc atcacgtgcc ctggacttat ggaatgaaaa
      661 gcggaaattc cctctgggac gaactttgct acacctatga ttcaggagta caggaggtga
      721 gagattatca gaaaacgtgg gataaactgg agaaatatgt cgatgcagaa agattcaggc
      781 acgttcagtc gcggttgaaa atccaatcga gagatgctgt gtggtggaaa gatgcttgcc
      841 tgctctattt caaacaattt tcccatatgc cgattcctta cgatatcgaa cgtccggttt
      901 gggaactgga ggagctgatg aaaataaaac tggatatgac gcaccataat taagtcagaa
      961 gcctgcctgc ctccggcagg tcagaaatta gaagtcagaa attagaaatt agaagtcaga
     1021 aagaacctca taattaaatc attttttcta tgaaaacgaa tgtatctaaa ttttcatttt
     1081 ttctgctgat aattttatta accggctgtg cttccgggaa aaaaacaaac gagtggattt
     1141 cgctttttaa tggaaaggac ctgacagggt ggacggtgaa aattaagggt tacaaagccg
     1201 gagaaaactt tggtaacacg ttcaggtttg aagacggcat actgaaagtg cgctatgatg
     1261 cctatgaaac ttttgacgat cggttcggac acatttttta caaagataaa ttttctcact
     1321 atattgtccg ttttgaatac cggattttgg gtgagcaatg tcccggcggt cctgagtggg
     1381 gctataagaa taccggaatt ttgattcacg ggcaaacacc tgaatcaatg gatttggacc
     1441 agaatttccc cacatccatc gaggtgcagt tgctcggtgg tgattctgtc ggagaaagat
     1501 ccaccggcaa tgtttgtacg ccgggaacca atattgtaat caataatcag ttgattctgg
     1561 agcattgcac caattcttcc ggcagcacta tccgaacgga tgagtgggtc agggtagagg
     1621 tggaagttca cggcgacagc atcatcaggc attttatcaa cgacgagcag gtgatagcct
     1681 acgaaaagcc tcagcttgat gaccgcgatg caacattttc aaaactgctt cctctcaacg
     1741 gtggaaaaat tcagctgacc gaaggaacca tctgcctgca aagcgaaagt catccgtgtg
     1801 atttcaggaa tgtgga
//
LOCUS       DAORKP010000269         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_25648_length_1794_cov_7.696952, whole genome shotgun sequence.
ACCESSION   DAORKP010000269 DAORKP010000000
VERSION     DAORKP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25648_length_1794_cov_7.696952"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..85)
                     /locus_tag="PLN75_09720"
                     /pseudo
     CDS             complement(1..85)
                     /locus_tag="PLN75_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            146..523
                     /locus_tag="PLN75_09725"
     CDS             146..523
                     /locus_tag="PLN75_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRG04432.1"
                     /translation="MKSYIQSVIVDKNQTAYSLGSGSLDVFATPALVAFMENTAVKAL
                     DDLGEGMTTVGTVINVRHLKASKIGEKMTCTATLIHQEGRRYEFEITVTDSSGELTAI
                     ATHERAAVDVERFLRKLNAGKDE"
     gene            complement(679..>1794)
                     /locus_tag="PLN75_09730"
     CDS             complement(679..>1794)
                     /locus_tag="PLN75_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRG04433.1"
                     /translation="RTGSGETVTTGGSGFGLMALLVGIERGFITREQGFQRLNTTVNF
                     LSEKAERFHGAFPHWLNGTSGKTIAFSTKDNGADLIETSFLIAGLLTVQSYFASGNSA
                     EKLMCAKIQQLWEGVEWTWFQKNNEPRLYWHWSPNYGWDMNMPITGWNEGLITYVLAA
                     SSPTFSITKNVYTEGWARNGAMKNGNKFYDITLPLGENRGGPLFFAHYSFLGLDPRKL
                     ADEYANYWQQNTAHAKINYAYCVSNPFGYFGYSSSCWGLTASDIPNGYTASSPTNDRG
                     TIAPTAALASFPYTPEESIRAMRFYYYVLGDKLWGEYGFRDAFNLSSQWFANSYLAID
                     QGPIVVMIENYRSGLLWNQFMQRDDIQKGLTKLGFTF"
BASE COUNT          495 a          395 c          411 g          493 t
ORIGIN      
        1 atactgaaat tgcagctctt cgcatctggt taacaattcc atatttttct tcaggcggat
       61 aactttttgt tacctgataa atttttgtaa caagtttgta acattagctg caattttcag
      121 tttaatatgt aaaaatcaat ctgacatgaa atcgtacata caatcggtga tagttgataa
      181 aaatcagacc gcatattcac tcggttcggg aagtctggat gtttttgcta cgcctgctct
      241 ggtagcattt atggaaaata cagctgtgaa ggcattagat gatttgggcg aaggaatgac
      301 aaccgttggc acagtaataa acgtaaggca tctgaaagcg agtaaaatag gcgaaaaaat
      361 gacctgcaca gccacattaa ttcatcagga aggaagacga tacgagtttg aaatcaccgt
      421 cactgattca tctggcgaac ttacagcaat cgccacccat gagcgggcag cggtagatgt
      481 tgagcggttt ttacggaaat tgaatgcagg aaaagacgaa taactgaaga acgataacgg
      541 tcggaataaa aatgattgag ttctgagttc tgaatttcaa gtttcaagta tttctgtgtc
      601 tgtcttctga cttgccagaa acccagacat atcggcctgc ctgctgcagg cagggataaa
      661 ccaggcaagt ttctgacttc aaaacgtaaa tccgagctta gtcaatcctt tttgaatatc
      721 atcacgctgc ataaactgat tccagagcag tccgctgcgg taattttcaa tcatcaccac
      781 aatgggaccc tgatcaatag ccagataaga atttgcaaac cactggctgc tcaaattgaa
      841 tgcgtcgcga aaaccgtatt caccccaaag tttgtcaccg agtacgtagt agtaaaagcg
      901 catggctctt atgctttctt ctggagtgta cggaaacgaa gcgagggctg cagtcggagc
      961 tatggtacct ctgtcgttgg tgggcgatga agcggtgtat ccgttcggga tatcgcttgc
     1021 cgtaagtccc cagcatgagc tgctgtaacc aaaataacca aaaggattgc ttacacagta
     1081 tgcataattt attttggcat gagcggtgtt ttgctgccag tagttggcat attcatcggc
     1141 cagttttcgc ggatcaagcc ccaaaaagga atagtgggca aaaaatagcg gtcctcccct
     1201 gttttcgccc agcggaagag tgatgtcgta gaatttattt ccgtttttca tcgctccgtt
     1261 tcgtgcccag ccttcggtat aaacgttttt tgtaatcgaa aatgtgggag atgaagccgc
     1321 cagcacgtaa gttatcaatc cttcattcca tccggtgatg ggcatattca tgtcccagcc
     1381 gtaatttggc gaccagtgcc agtaaagcct gggctcgttg tttttctgaa accaggtcca
     1441 ttccacacct tcccatagtt gctgtatttt ggcacacatt agcttttcgg cactgttgcc
     1501 ggaagcaaaa taggattgta ccgtgagcaa tccggctatc agaaacgatg tttctatcag
     1561 atcggctcca ttatctttgg tactgaaagc aatggttttt ccgctggtgc cattgagcca
     1621 gtgtgggaat gcaccatgaa accgttcggc tttttcggag agaaaattca cggtcgtatt
     1681 caatcgctga aatccctgtt cgcgtgtaat gaatccccgt tcaatcccca caagcagcgc
     1741 cataagacca aaaccggaac cgcccgtagt gactgtttcg ccggaacctg tacg
//
LOCUS       DAORKP010000270         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_25837_length_1787_cov_7.864896, whole genome shotgun sequence.
ACCESSION   DAORKP010000270 DAORKP010000000
VERSION     DAORKP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25837_length_1787_cov_7.864896"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLN75_09735"
     CDS             <1..617
                     /locus_tag="PLN75_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRG04434.1"
                     /translation="DMTGERRVEQRKEVFRHLEKVLSPQAIIASNVTTVIVSDLASDL
                     ENKSRCIGLHFLSNVPDSQMIEIVRGLETSDATYEKVCQFARLINYQYVPVVESAGLV
                     SIRLFLIQLNEACSILMENISNTEDIDRIMTVGFGHRQGLFRIADRLGIEKIVKLLEN
                     LYDEYGHVKYKTSPILLRMYRAKHFGVSTKKGFYTYDDMGNIIK"
     gene            634..>1787
                     /locus_tag="PLN75_09740"
     CDS             634..>1787
                     /locus_tag="PLN75_09740"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRG04435.1"
                     /translation="MKILVLNCGSSSVKYKLLDMTSKEVLGSGGVEKIGMKGSFLKHT
                     KQNGEKVILEGEVLEHQLAIEYILGVLTSEKHGCIKNMEEIDAVGHRVVHGGEKFNSS
                     VVITDEVIRNIEACIEIAPLHNPPNLAGIFAISDLLPQIPQVAVFDTAFHQTMPEHAY
                     MYGIPYSLYKKYGIRRYGFHGTSHRYVSRRACEFLGLDYNKTKIITAHIGNGGSITAI
                     ENGKSVDTSMGFTPVEGLMMGTRSGDVDLGVVTYLMEKEMISTSATTTLFNKHSGVLG
                     VSGISSDMRDIEKAISEGNQRAKLALGMYEYRIKKYIGSYAAVLNGFDVLVFTGGVGE
                     NQTITRERICNGLSYLGIEIDPEINRIRGEELLISTSNSRVKVVVIPTDE"
BASE COUNT          545 a          349 c          428 g          465 t
ORIGIN      
        1 acgatatgac cggcgaacga agagtggagc aacgtaaaga agtattccgc catctggaaa
       61 aagtactgtc gccacaggca atcatcgcct ccaatgttac tactgtaatt gtttcggacc
      121 tggcttcaga ccttgagaat aaaagccgct gtatcggact gcatttcctc tcgaatgtgc
      181 cagactcgca aatgatagaa atagtacgcg gtctcgaaac atcggatgca acctatgaaa
      241 aagtctgtca gtttgcccgc cttatcaact atcagtatgt tcctgttgtc gaatcggccg
      301 gattggtcag catccgtctt ttcctgattc aactcaacga agcctgttcc attctgatgg
      361 aaaatatttc aaataccgaa gatattgacc gcatcatgac tgttggattt ggtcaccgac
      421 agggattatt tcgtatagcc gaccgccttg gaatagagaa aatcgtaaaa ctgcttgaaa
      481 acctgtacga tgagtatgga catgtgaaat acaaaacttc accgatatta ttacgtatgt
      541 atcgtgctaa acatttcggg gtaagtacca aaaaagggtt ttacacctac gacgacatgg
      601 gaaatattat aaaataatgg aggaaagtaa gttatgaaaa tattggtatt gaattgcggc
      661 agttcttcgg tgaagtataa actgttggat atgacaagca aagaagtgct tggatcggga
      721 ggggtggaaa aaatcggtat gaaaggatca tttcttaagc ataccaaaca aaacggagaa
      781 aaagtaattc ttgaaggtga agtccttgaa catcaactgg ctatagaata tattctgggt
      841 gtactcacca gcgaaaaaca tggctgcatc aaaaacatgg aagaaattga tgccgtaggg
      901 catcgtgtag tgcatggagg cgaaaaattt aactccagtg tggtaattac cgacgaagtg
      961 atacggaata ttgaagcttg tatagaaata gctccccttc acaatccgcc gaatctggca
     1021 ggtatttttg ctatcagcga cttgctgcca cagattcctc aggtggcggt tttcgatact
     1081 gcttttcatc agaccatgcc cgaacatgcc tatatgtacg gaattcctta ttctctgtat
     1141 aaaaaatacg gtattcgccg gtacggtttt catggcacca gtcaccgcta tgtatcgcgc
     1201 agggcatgtg agtttttggg tctggactac aataaaacca aaatcattac agctcatatt
     1261 ggcaacggag gctctattac ggccatcgaa aacggaaaat cggtggatac ctccatggga
     1321 tttacacccg tagaaggtct gatgatggga acccgttcgg gtgatgtaga tttgggtgtg
     1381 gtgacttacc tgatggaaaa agagatgatc agtaccagcg caactaccac cctgttcaat
     1441 aaacacagcg gcgtgctggg tgtttcggga atttcgtcgg atatgcgcga tatcgaaaaa
     1501 gcaatatccg aaggaaatca acgggcaaaa ctggctctgg gtatgtacga atatcggatt
     1561 aaaaaatata ttggttccta tgcggcagta ctcaacggat ttgatgtact tgtatttacg
     1621 ggtggagtag gtgaaaatca aaccattacg cgtgagcgaa tctgcaacgg actgagttat
     1681 ttgggtattg aaattgatcc ggaaatcaac aggatacgcg gggaagaact gctgataagc
     1741 acatctaatt ctcgggttaa agtggttgtt atcccgaccg acgaaga
//
LOCUS       DAORKP010000271         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_27111_length_1737_cov_7.067776, whole genome shotgun sequence.
ACCESSION   DAORKP010000271 DAORKP010000000
VERSION     DAORKP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27111_length_1737_cov_7.067776"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLN75_09745"
     CDS             <1..197
                     /locus_tag="PLN75_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04436.1"
                     /translation="KNDIKPKKPRLGINASKIEGESYVGCKLENTTFKSSVFDSLHLI
                     LEKHDASKIKITHKENIIIL"
     gene            217..759
                     /locus_tag="PLN75_09750"
     CDS             217..759
                     /locus_tag="PLN75_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04437.1"
                     /translation="MPKTYSQDMLNNYLQGVIGNDFGNARYLKLEVGRKISFVEAGVG
                     FSHSSNLPFKKSNASVHIDNGNILSTYNANDLYGKQYFSLMVNAKVNLLHFLLKQKKH
                     QLMLGGSLGVSDGLKVMQYFENDSKKVAMDTERYFGIDNTVNLTYQYQICDKYALGVS
                     AEMLSLPEFLMFGVSLKRFF"
     gene            918..1646
                     /gene="gldF"
                     /locus_tag="PLN75_09755"
     CDS             918..1646
                     /gene="gldF"
                     /locus_tag="PLN75_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRG04438.1"
                     /translation="MISIFKKEFRSFFSSATGYIVIGIFLLLTGLFLWVIPGSFNIPD
                     SGYANVDGLFYLAPWLFLFLVPAVCMRFFAEEKQSGTWELLLTKPLTGWQIVLGKYFA
                     GLVLVLLALLPTLIYYASVSYLAQPAGNVDSGQFWGSFIGLFFLAAVYAAIGTFSSSL
                     SNNQITAFITALVLCFFFYYGFDVLSGFFSSGIQRQFIENLGIHAHYKSISRGVIDSR
                     DMAYFVLLCTVFTGSTVWKIKRYF"
BASE COUNT          497 a          300 c          360 g          580 t
ORIGIN      
        1 caaaaaatga tattaaacca aaaaaacctc gactgggaat aaatgcaagt aaaatagaag
       61 gtgaaagtta tgtgggctgt aaactagaaa acacaacttt taaaagttca gtttttgatt
      121 ctttacatct tattttagaa aaacatgatg cctcaaaaat aaagattacc cataaagaaa
      181 atattattat tttatagcat aatatttttt gcttttatgc ctaaaacata ttctcaggat
      241 atgttgaata attatcttca aggcgttatt ggcaacgact ttggcaatgc ccgttatttg
      301 aaacttgaag taggaagaaa aatatccttt gtggaagcag gcgtaggttt ttcacattca
      361 tccaacttgc cttttaaaaa atcgaatgcg agcgtacata ttgataatgg gaatatcctt
      421 tcgacttaca atgcgaatga cttatatggg aagcagtatt tttcgctgat ggtaaatgcc
      481 aaagtgaacc ttctgcactt tttgttgaag caaaaaaaac atcaacttat gttaggaggt
      541 tcgttaggag tttccgacgg gctaaaagta atgcagtatt ttgaaaatga tagcaaaaaa
      601 gtagctatgg atacggaaag atatttcggt attgataata cggttaatct tacttatcaa
      661 taccaaattt gcgataaata cgcattggga gttagcgctg aaatgctgtc gttaccggaa
      721 tttttgatgt ttggcgtttc gctaaaaaga tttttttagt cgcgcttttg tatcattgag
      781 aaaacaggaa acattcatgg ataaaatcta tggatgtttc ttctttttaa acctaatgac
      841 tgtgcaaaat gcgcaaaggt ttgataactc gttaaatttt tgctatcttt gacagcaaat
      901 tagcagcaat tgtacaaatg atttccattt tcaaaaaaga atttcgctcc tttttcagta
      961 gtgcaaccgg ttacatcgta atcgggattt ttttattgct cacggggttg tttctctggg
     1021 ttatacccgg ttctttcaac atacccgaca gcggctacgc caatgtcgac ggacttttct
     1081 acctcgctcc ctggcttttc ctttttcttg taccggctgt ctgtatgcgg ttttttgccg
     1141 aagagaaaca gagcggaacc tgggagttgc tgcttactaa accgctgaca ggctggcaga
     1201 tagtactcgg aaaatatttt gccggattag tactggtatt attggctctt ttgccgacgc
     1261 ttatatatta cgcttcggtg tcgtatttgg ctcagccggc aggtaatgtg gattcagggc
     1321 agttttgggg ctcgttcatc ggattatttt tcctggctgc cgtttatgcg gccatcggaa
     1381 cgttttcatc gtcgttgagc aacaatcaga ttacggcttt tatcactgcc ctggtgctgt
     1441 gtttcttttt ctattacgga tttgatgtac tgtccggttt tttcagcagc ggaattcaac
     1501 gtcaatttat cgaaaatctt ggcattcatg cacattacaa atccatcagc cgtggagtga
     1561 ttgacagcag ggatatggct tatttcgtgt tgctctgcac tgtttttacc ggaagtacgg
     1621 tgtggaaaat taaacgctat ttttgagttc tgtcccgata actatcggag agttcggagt
     1681 ttggagtttt gagttttgag aaaaaaaatc actattgtcc gataaaaaca gatatta
//
LOCUS       DAORKP010000272         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_28611_length_1685_cov_8.019632, whole genome shotgun sequence.
ACCESSION   DAORKP010000272 DAORKP010000000
VERSION     DAORKP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28611_length_1685_cov_8.019632"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1532
                     /locus_tag="PLN75_09760"
     CDS             333..1532
                     /locus_tag="PLN75_09760"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRG04439.1"
                     /translation="MESIRELYRIGHGPSSSHTMGPRFAAERFVAQTPEAVSYRVSLY
                     GSLAATGRGHLTDATIAETLQSKAVEFIWLPEIVLPFHTNGMKFEALDADGLVQKDWV
                     VYSVGGGKIVDETIVNEENRIYEHSTIAEIMPWIEKRGQTYWEYVQDREGKDIWDFLN
                     EIWKAMQQSVRDGLEADGVLPGELYLRRKAASYWIKAKGYKVSLQNRCLIMSYAMAVS
                     EQNASGGLVVTAPTCGSSGVIPGILYHLKTYHEMSDKHILQALATAGLFGNVIKTNAS
                     ISGAEVGCQGEVGSACSMASAASCQLFGGSPQQIEYAAEMGLEHHLGMTCDPVCGLVQ
                     VPCIERNAFASLRALDANLKSMLSDGKHLISFDKVVEVMKKTGHDLPHLYKETAEGGL
                     AKEWLVD"
BASE COUNT          506 a          333 c          414 g          432 t
ORIGIN      
        1 gataagaata tttgaaagat catttttgta taaacatctg atagtcaata tatgcgattt
       61 tatattaatc agtatttcaa gcatttatat acttgcgaaa gtttaatttt ttagaataaa
      121 taaattccaa aagtttgaca aaattaagaa aaatattaac atgttcatcc ttgaaggaca
      181 gctaaattgt cattttagtt ataaatactg aaagtgaagt tttggtattg attttattcc
      241 taattttgta gttgaataga gaaaagccta tctgaaagtt aaggctaaaa ataatactct
      301 acattcatca atcaaaataa tcccgaatta aaatggaatc aatcagagaa ttataccgca
      361 taggtcacgg gccttcgagc agtcatacga tgggaccgcg atttgcagcc gaacgatttg
      421 tggctcagac gcccgaagct gtttcgtacc gcgtgagcct gtatggcagc ctggccgcta
      481 ccggcagagg ccatctcacc gacgcaacca tagccgaaac cttacaatcc aaagcagtgg
      541 aattcatctg gctgcccgaa attgtactac cgtttcacac caacgggatg aaatttgaag
      601 cactggatgc tgatgggctt gtacaaaaag actgggtggt atatagcgtt ggcggaggaa
      661 aaatagtgga tgaaacgatt gtaaatgaag aaaaccgaat ctacgaacat tcaactatag
      721 ctgaaataat gccctggatc gaaaaacggg gacaaactta ctgggagtac gtgcaggata
      781 gggaaggaaa agacatctgg gattttctga acgaaatctg gaaagccatg caacaatcgg
      841 tgagagacgg actcgaagcc gacggagtgc tgcccggtga attgtatctg cgacgcaaag
      901 ctgcatcata ctggataaaa gcgaaaggtt ataaagtcag tttgcaaaac cgctgtctga
      961 taatgtctta cgctatggct gtgtccgagc agaatgccag cggcggacta gtggtgacgg
     1021 cacccacgtg cggttcttcg ggggtgatac cgggcattct gtatcatctg aaaacgtatc
     1081 acgaaatgtc ggataagcac attcttcagg ccctcgctac tgcaggttta ttcggcaatg
     1141 taatcaaaac aaatgcttcc atttcgggag cggaggtagg ttgtcagggc gaagtaggtt
     1201 ctgcctgctc catggcttcg gctgcttcct gtcagctttt cggcggaagt ccgcagcaga
     1261 tagaatatgc tgccgaaatg ggccttgagc atcacctcgg catgacgtgc gaccctgtgt
     1321 gcggattggt gcaggtgcct tgtattgagc ggaatgcatt cgcttcgctt cgtgcactgg
     1381 atgccaacct gaaatcgatg ctctccgatg ggaaacacct catcagtttt gacaaagtgg
     1441 tggaagtaat gaagaaaacc ggtcatgacc tgcctcatct ctacaaagaa acggccgaag
     1501 gcgggctggc aaaggagtgg ttggttgatt agacactaag caccctaaaa tctcccattt
     1561 tggcgggcaa gctccgaaat gcggacttag aaataaggtg tttcctggtt gatttagtct
     1621 gatgaaaggg aaaataaatc gaaaaaagtg gaatccggta taggcggaat tccacttttt
     1681 tattg
//
LOCUS       DAORKP010000273         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_29230_length_1665_cov_9.030435, whole genome shotgun sequence.
ACCESSION   DAORKP010000273 DAORKP010000000
VERSION     DAORKP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29230_length_1665_cov_9.030435"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1665
                     /locus_tag="PLN75_09765"
     CDS             <1..>1665
                     /locus_tag="PLN75_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRG04440.1"
                     /translation="SKFTTDYYGKYVFTKSIMAKYLSKDIMKALNNAIDKGVTLDRNI
                     ADDVATGMKMWAMELGATHYTHWFQPLTDGTAEKHDSFIDYAAGPGEAIIEDFSGKLL
                     AQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFVVDDTLCIPTIFISYTGESLDMKA
                     PLLKSLNAVNKAATAVCQMFDPNVKKVTAYLGWEQEYFLIDEGLYATRPDLMLTGRTL
                     IGHESAKNQQMEDHYFGSVPTRVAAYMKDLEFEAYKFGIPVKTRHNEVAPNQFELAPI
                     FGECNLAVDQNLLVMSIMKKVARRHGFRLLLHEKPFAGINGSGKHNNWSLGTDTGVGL
                     LTPGKTPAENLQFITFLVNVLMAVYKNNALLKASIMTAQNAHRLGANEAPPAIISAFL
                     GKQITAVLDKLENSTSDEAIVVSDKQQLSLGIAHIPEVLLDNTDRNRTSPFAFTGNRF
                     EFRAVGSSANCSSAMATLNAAVAAQLTEFKIAVDAKIAAGSTEQSAIFEILKEYIKIS
                     KPIRFDGNGYSEEWKVEAAKRGLDCETRVPEIYKAFTSEQSVKMFT"
BASE COUNT          479 a          389 c          371 g          426 t
ORIGIN      
        1 cttcgaaatt caccaccgat tactacggta aatatgtatt caccaaatcc attatggcca
       61 aatatctgtc caaagatata atgaaagcgc tgaataatgc cattgataaa ggagttactc
      121 ttgatcgaaa tatagctgat gatgtagcta ccggcatgaa aatgtgggct atggaacttg
      181 gagcgacaca ctacacgcac tggtttcagc ctctgaccga cggaacagcc gaaaaacatg
      241 actcgttcat cgactatgca gccggacccg gtgaagctat tatcgaggat ttttcgggaa
      301 aactcctggc acaacaggaa cctgatgcat cgtcctttcc gagcggtggt attcgcagta
      361 cattcgaagc ccgcggatac acagcctggg atccttcgtc acctgctttt gtggtggatg
      421 atacactttg tattcctacc atattcatct cctataccgg cgaatcgctc gacatgaaag
      481 ctccattgct caaatcgctt aacgctgtca acaaagcagc cacagcggtt tgtcaaatgt
      541 ttgacccgaa cgtaaaaaaa gttactgctt atcttggctg ggaacaggag tattttctga
      601 ttgacgaagg attgtatgct acccggcctg accttatgct caccggtcgt acattgatcg
      661 gtcacgagtc tgccaaaaat cagcagatgg aagaccatta ttttggttct gtacctaccc
      721 gtgttgcagc ttatatgaaa gatctggaat ttgaggctta taagttcggt attccagtga
      781 aaacccgtca caacgaagta gcccctaacc agtttgagct tgctcctatt tttggagaat
      841 gtaacctggc tgtcgaccaa aatctgctgg tgatgtccat tatgaaaaaa gtggcacgtc
      901 gtcacggttt tcgtctgctg ctgcatgaaa aaccctttgc tggcattaac ggctccggga
      961 aacacaataa ctggtcactc ggaactgata caggcgtcgg attgcttaca ccgggaaaaa
     1021 ctccggcaga aaatcttcaa tttatcacct ttttggttaa cgtcctaatg gccgtatata
     1081 aaaataatgc cttgctgaaa gcatccatta tgacggctca gaatgcgcac cggttaggtg
     1141 caaatgaagc accgccagcc atcatttcag cgtttctcgg aaaacagatt acagccgtgc
     1201 ttgataaact ggaaaacagc accagcgatg aagcaattgt agtgagcgac aaacaacaac
     1261 tgagtttggg tattgctcac attccggaag tattgctcga taataccgac cgcaaccgta
     1321 cttcaccgtt tgcttttacc ggtaatcgtt ttgaattccg agcagtgggt tcatccgcca
     1381 actgttcctc agcaatggca actctgaatg ctgccgtagc cgcccagctg actgaattta
     1441 agatagctgt ggatgctaaa atcgctgccg gctccaccga acagagtgcc atatttgaaa
     1501 ttctgaaaga atatatcaaa atctcaaaac caatacgttt tgacggcaat ggttatagcg
     1561 aagagtggaa agtcgaagca gcgaaacgcg gactggattg cgaaacaaga gttcctgaaa
     1621 tttacaaggc atttacgagc gaacaaagcg tgaaaatgtt cacct
//
LOCUS       DAORKP010000274         1611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_30939_length_1611_cov_7.663882, whole genome shotgun sequence.
ACCESSION   DAORKP010000274 DAORKP010000000
VERSION     DAORKP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30939_length_1611_cov_7.663882"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLN75_09770"
     CDS             <1..321
                     /locus_tag="PLN75_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRG04441.1"
                     /translation="APFVSMVRSGQEIGKILIHGGRYDENFYFSDLLENKLSGNYIRC
                     ATQQADSAYYKGRLTGWLREQSFLPTELKYYLCGSPEMVVEVRDILISRHIPFGQIIS
                     ETYF"
     gene            333..1376
                     /gene="rlmN"
                     /locus_tag="PLN75_09775"
     CDS             333..1376
                     /gene="rlmN"
                     /locus_tag="PLN75_09775"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRG04442.1"
                     /translation="MDKQPLFGLSLQKIKYVVASLALPSFTATQITDWLYKKHVSSID
                     EMTNLSKKARELLNEKFIVGLTAPSNVQISTDGTKKYLYPTATEKFVEAAYIPDEDRA
                     TLCVSTQIGCKMGCLFCMTGKQGFQGNLTAGEIVNQIQSLPEFENLTNIVYMGMGEPL
                     DNLQPVLDSLEILTASWGYAWSPKRITVSTIGVIPAMQTFLKQSNAHLAVSMHTPFDD
                     ERKRIMPIQSVYGISEVVSELRKWDFSHQRRVSFEYIMFKGVNDTPRHAREIVRLLEG
                     IRCRINLIRFHPIPDTALEGTPYNDMVEFQNMLKARGMTVTIRASRGEDIYAACGMLS
                     TKELVKRQQEPDF"
BASE COUNT          456 a          361 c          367 g          427 t
ORIGIN      
        1 gctccgtttg tttccatggt ccgttcggga caggaaatcg ggaaaatcct cattcacgga
       61 ggccggtacg atgaaaattt ctatttttcg gatttgctcg aaaataaact ttccggaaac
      121 tacatccgct gtgccacgca gcaagctgat agtgcctatt acaaaggaag actgaccggc
      181 tggctcaggg aacaatcgtt tttgccaacc gaattaaaat actacctttg cggaagtccc
      241 gaaatggtag tagaagtcag agacatactg atttccagac acataccttt cggtcagatt
      301 atttcagaaa cctattttta gtcaataaaa aaatggataa acaacctctt ttcggcttat
      361 ctcttcaaaa aatcaagtat gtagtcgctt cactggcatt gccgtcgttt acagccacac
      421 aaatcaccga ctggctttat aaaaagcacg tgagttcgat tgacgaaatg accaatctgt
      481 ccaaaaaagc gcgtgagctg ctgaatgaaa agtttatcgt cgggcttaca gctccttcca
      541 acgttcaaat ctcaacggat ggcacaaaaa agtaccttta ccctacggct accgaaaaat
      601 ttgtagaagc tgcctacata ccggatgagg acagagccac gctttgtgtt tcgactcaga
      661 ttggctgtaa aatgggatgt ttgttttgca tgaccggtaa gcagggcttt cagggcaatc
      721 ttacagcagg cgaaattgta aaccagatac aatcgttgcc cgaattcgaa aatctgacca
      781 acatcgtgta catgggcatg ggtgaaccgc tggacaacct gcagcccgtg cttgatagcc
      841 tcgaaattct gactgcctcg tggggatacg cctggagtcc gaagcggatt actgtttcca
      901 ccatcggggt cattccggcc atgcaaacgt ttctgaaaca aagtaacgct caccttgccg
      961 tgagcatgca tactcctttt gacgacgagc ggaaaagaat catgcctata cagagtgtgt
     1021 atggcatctc cgaagtagtt tcagaattga gaaaatggga tttcagtcat cagcggcgtg
     1081 tttcgtttga atacatcatg ttcaaagggg taaatgacac accccgccat gccagagaaa
     1141 tagtacgtct gctggaagga ataaggtgcc gcatcaacct gattcgtttt cacccgattc
     1201 ctgacactgc gctggaaggc actccttaca atgacatggt ggaatttcag aatatgctca
     1261 aagccagggg aatgactgta accattcgcg cttcgcgggg tgaagatata tacgctgcct
     1321 gcggaatgct ttccaccaaa gaactggtaa aacggcagca ggaaccggat ttttaattgg
     1381 tgggcatctt tttaaaatga ttatccgcaa aatgtcctga catatcatta gttgccacac
     1441 cttttaaggc gtgggtatga atctcaggtc atcctgcgga taatcttttt ttaataatca
     1501 actttcgcaa atgtaagtag ttgtaattaa ggtatataca attatgctta caattttcta
     1561 cttgcgatga cctgtccccc tctccttggg agaggggtta ggggagaggt c
//
LOCUS       DAORKP010000275         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_5522
            NODE_31991_length_1580_cov_10.301639, whole genome shotgun
            sequence.
ACCESSION   DAORKP010000275 DAORKP010000000
VERSION     DAORKP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867739
            Sequence Read Archive: SRR1144841
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:01:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,958
            CDSs (total)                      :: 1,928
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31991_length_1580_cov_10.301639"
                     /isolate="AMR_MDS_5522"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLN75_09780"
     CDS             <1..920
                     /locus_tag="PLN75_09780"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRG04443.1"
                     /translation="LEHCEQVSVVVRSTANIYFNLQQVHVFTGDFTDYETLKKAASGC
                     DAVIHAAAVTATHLLHYSDYEKVNVRGSANVLRACNELGINNIVFISSSNTIGYGTSE
                     NPSNEHSPIQFPFIHSHYAQSKLAAEQLFFEASQMPDKHIIIINSCFLIGGYDTKNSS
                     GRLVKMGYKKPVLFIPRGGKNFVSARKVARAACNALTMGKNGEKYLVCGENLSFRDFF
                     ERQKYAGKYRQIMIRLPDFLLLFAGRIGDMLRKTGIKTDLSLVNMRQLIIREFYTGKK
                     ADENLHLPQFHLENAIIEAINWINIKKKV"
     gene            964..1461
                     /locus_tag="PLN75_09785"
     CDS             964..1461
                     /locus_tag="PLN75_09785"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HRG04444.1"
                     /translation="MKKLSKFIIFSVILLLNVSCAAKKSAAELSEQRRILVEKIENQQ
                     FKFIASYAIPRGNFQPRYLTSTYDVVVTRDTVYSHLPYFGVAYQAPWDPSESPLIFNS
                     TKFDYSVTEGRRSGSWNINIRIHDRKNPMVYIFTLWENGSGDLVVWDSSRQSISFRGD
                     VELNP"
     gene            complement(1465..>1580)
                     /locus_tag="PLN75_09790"
     CDS             complement(1465..>1580)
                     /locus_tag="PLN75_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRG04445.1"
                     /translation="GPGKSSRSHTADEFVEIKEIEQGISVYYELLSGLNLY"
BASE COUNT          480 a          319 c          319 g          462 t
ORIGIN      
        1 tgctcgaaca ttgcgagcag gttagtgtcg ttgtgcgaag tactgcgaat atttatttca
       61 atttgcagca ggttcatgtt tttacaggtg attttaccga ttatgaaact ttgaaaaaag
      121 cagcttcagg ctgcgatgct gtcattcatg ctgcagccgt cacagctacc catttgctgc
      181 attattcaga ttacgaaaag gtcaacgtgc gaggatcggc caatgtgctc cgcgcctgta
      241 atgaacttgg tatcaacaat attgttttta taagttcctc caacacaata ggttacggaa
      301 cttcagaaaa tccgtcaaac gaacactctc ccattcagtt tccattcatc cattcacatt
      361 atgcccaaag caaattggcg gccgagcaat tatttttcga agcttctcaa atgccggata
      421 aacacataat catcatcaat tcctgttttc ttattggcgg atatgatacc aaaaacagtt
      481 ccggaaggtt agtaaaaatg ggttataaaa aacctgttct tttcattccc aggggaggaa
      541 aaaattttgt ttctgcccga aaagtggccc gggcagcttg caatgcgctt acaatgggaa
      601 aaaatggtga aaagtacctg gtttgcggcg aaaatctcag tttccgggat tttttcgaaa
      661 ggcaaaaata tgccggaaaa tatcgtcaga taatgattcg tttaccggat tttcttttac
      721 tttttgcagg caggataggt gatatgttgc gaaaaacggg aataaaaacc gatctcagct
      781 tagtaaatat gcgccaactt atcattcggg aattttatac aggcaaaaaa gcggatgaaa
      841 acctccacct tcctcagttt catctcgaaa atgctattat tgaagcaata aattggataa
      901 atattaagaa aaaggtataa attttgtact taaatttaac aatcaattaa tatcattgaa
      961 gctatgaaaa aactatctaa atttatcatt ttcagcgtaa tattactgct gaatgtttcg
     1021 tgcgccgcca aaaagtctgc tgccgaatta tctgaacaaa ggcgaattct ggtagaaaaa
     1081 atagagaacc aacagttcaa atttattgcc agctacgcta ttccgagagg aaactttcag
     1141 ccgcgttatc ttacctcaac ctatgatgta gttgtaacca gagacactgt ttattctcat
     1201 ttgccatatt ttggtgtagc ctaccaggct ccgtgggatc cgtccgaatc gcccctgatt
     1261 ttcaacagca ccaaatttga ctattcggtt acagagggca gacgcagcgg aagctggaac
     1321 attaacatcc gcatccacga ccgtaaaaat ccaatggtat atatttttac cctctgggaa
     1381 aacggaagcg gcgatttggt tgtgtgggat tcgtccaggc aatcgatatc tttcagaggg
     1441 gacgtagagt tgaatcctta agaactaata aaggttcaat ccgctcagta gttcataata
     1501 aacgcttatt ccctgctcaa tttcttttat ttcaacaaat tcatcggcgg tgtgtgagcg
     1561 ggaagatttt ccggggccta
//
