LOCUS       DAOROD010000001       167230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1_length_167230_cov_34.388300, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000001 DAOROD010000000
VERSION     DAOROD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 167230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 167230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..167230
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_167230_cov_34.388300"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1611
                     /locus_tag="PLW53_00005"
     CDS             169..1611
                     /locus_tag="PLW53_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD35841.1"
                     /translation="MKQKMNAKAFKALIAMAFAISASVVPLSQSFAAGESLGKSCTTE
                     GLMTGTKSTSLVCVKGGNGKLTWQRVKLASGTGRPVANLTPPKGEIEFWHYRVELADQ
                     RALVKIISDFEAKYPGTKIKQVVRDTTTYNNTARVAILANPKAALFATSRGAIFDQFA
                     KSDMMLDLSDQRYVKQNVVAKGLTAGQYEGKQLGIPYQYLFNNPIVNTEIWAKEKWTT
                     PKNLTGWVNWCKDAKAKGYVPLAWPGATRGQAAQISNSALMNSAGSYDELAKNLADLN
                     SGKIDLTSKWFEGVADIYVKLRNAGCFPDNATGVTEAAAYNLFATGKSPILPIGTFAM
                     GSIVGLNPNLNGKMELMSMVLTDGKVVAEGIMNNTYTLSVNKNSALRDQKIANAFLSY
                     LLTGKVAEIYANETLQHVNVLDVEYKNVNLLNTVSFQAKNTMLAPRFLLLNAAVSDLT
                     QDALIQIVGGKSPADVLPDFSRQIKQKLAG"
     gene            1713..2555
                     /locus_tag="PLW53_00010"
     CDS             1713..2555
                     /locus_tag="PLW53_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD35842.1"
                     /translation="MGLPAAIIFAGFYFVPFIANLRYSLTRWDRITEPEFVGFRNFIN
                     LLTNDDLFYKVLGNNLRFTFLVVLFQTLFSLIFAIFLVKNTRANIALRTLFFFPTILS
                     SVSVGMIWLFMYDPNFGAINLFFTRLGLEGFALNWLGSESSALYAITFTQVWFHTGQM
                     MVIYIAGLQQIPQELYEAAEMDGASRWQQFRNVTWPMSIPTTIVVMAYTTIQSFRAFD
                     LIFAMTQGGPNNSSNIFAVLIYQTVFSELRIGYAATQSIFFIIVLVFVTLLQRKFLNS
                     RYEK"
     gene            2565..3380
                     /locus_tag="PLW53_00015"
     CDS             2565..3380
                     /locus_tag="PLW53_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRD35843.1"
                     /translation="MIYKVSRIVSLVIFSVLIIIPSLIVVFGSTKTDSEVYNKPLALP
                     EKWSLDNFRFLFEVSNVGRSFLNSLIVTGFSVTITLILASMCAFAISRMITVTGRVLF
                     SLFAVGLAIPGQVNLVPIFILFNDLQLTNKLSGLVLINIVTTLPISVFILTAFFKELP
                     KEMYEVSEVDGATPLRVYRSIALPLSRPAMGATAIFLFVITWNELLFPLLLITDNDKR
                     TLPLSLLSFRGEFFSNYSMIFTAVMVASIPMVVMYLLMQRSFIAGLTAGAVKG"
     gene            3380..4099
                     /locus_tag="PLW53_00020"
     CDS             3380..4099
                     /locus_tag="PLW53_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRD35844.1"
                     /translation="MSSHTPVVSNPPRLQGQVRLLLEHIESQGLQGGDRLEPERELAK
                     ILGISRPSLREAIQILQVQGRLRVKHGIGIFVLDQADGEKLRESLRAAQHRIEELFQM
                     REILEAPAVEWAAERRSEEQLAEMKLAAKALNEAISKKPVDFENLRRLDMEFHLTIVR
                     SAQNQFLNQTLGTLQEIMYHSMDNTLKLPGRVDSSEHEHGMILDAIEKRDSITARLMI
                     IQHIHNARDAWQKYLKKQSDL"
     gene            4096..4941
                     /locus_tag="PLW53_00025"
     CDS             4096..4941
                     /locus_tag="PLW53_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD35845.1"
                     /translation="MSQVLCVGVATVDNIVLVDKYPAANQRVVALDSTRAVGGPATTA
                     AVTLARLGIEVSLSCVIGDDEEGRFIFETLKNEGIDTSHVVVDANVRTARGTIVVSKS
                     EQSRAIMVMPHDQIPKKPLDTSKYQWIHVDQAGMRALKTWGISRGGSAKLSIDIGYAT
                     EGLNPGDYDLYAPSENITTDTTSAVKDKNIVVISQGEKGSLYCDGLSSGSVPAINTEI
                     LSTLGAGDVFHGALVAAQVWNKEISEAVLIANTVAGLSCRGLDGQSGIPRKNELDAYL
                     AKAKI"
     gene            4982..5281
                     /locus_tag="PLW53_00030"
     CDS             4982..5281
                     /locus_tag="PLW53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD35846.1"
                     /translation="MAKVPDFLLHHDGDSVAVANDDLQPGTLQGLSVKEQTAHTAVLN
                     EPIPLGHKFALRDHAEGEAIVKYGIKVGIASKAIKKGDYVHTHNMRSYRWEASRA"
     gene            5260..6426
                     /locus_tag="PLW53_00035"
     CDS             5260..6426
                     /locus_tag="PLW53_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD35847.1"
                     /translation="MGSKPRLTGYRRPDGSMGIRNHVIILPVDDLSNAAAEAVAKVVP
                     GTLALPHSYGRLQFGEDLELTFRTLIGTGLNGNVAAVVVIGIEPNWTQRVANGIAKSG
                     KPVASFSIEGKGDLQTIADAARVAQVFLQDASEIVRESASEGDLILSIKCGESDTTSG
                     LGSCPTTSEAVDRWVAAGGTVFFGETSELTGGEHLIADRCIDDACRNLFQTTYDNYIK
                     VIESTGANLLGSQPTQGNIAGGLTTIEEKALGNIAKTGSVPVVGVLAPAEAPPRNKPG
                     LYFMDTSSAAAECVTLMAAAGAVIHLFPTGQGNVIGNPIEPVLKLTANKKTAASMPEH
                     IDLDVSGLLRQEYNLTKAGDMMMDVIERTVNGRLCAAEALGHREFTFTKLYISA"
     gene            6465..7418
                     /locus_tag="PLW53_00040"
     CDS             6465..7418
                     /locus_tag="PLW53_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRD35848.1"
                     /translation="MILISEDVWSADFEALANSFPLIREPELWSNAEELKAKLESATA
                     LVVRNRTQVSRDLIASAKNLKIIARAGVGLDNIDVKAADEFGVVVSAALGINATAVAE
                     ETLAMALALSRKLIELDSSTRAGQWNRKAGVEISGKTWGLLGFGATARATAELLRGFK
                     VKVLAYDPFAKPEQNYLDSINCQMSSLDDVISNSDFISVHLPATSETKEIIDSKKLSV
                     MKKSALLISVGRGEVINEADLEQALRDGVIAGAGLDVRAQEPPKDKRFTDLDNVVLAP
                     HIAGITHESQAAINRVLVSEIRAALSGQVQSYAVGAVKQAK"
     gene            7418..8428
                     /locus_tag="PLW53_00045"
     CDS             7418..8428
                     /locus_tag="PLW53_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRD35849.1"
                     /translation="MAISIARALAHATSLLIKAGVNEVNSEKTARAIVTSDVWGNPSH
                     GLMRLPFYLQRLSQGGVNPKAELKVISEFGSTISLDGQDGLGHWQLLDGAQIGVTKAK
                     QSGISLVSIANSSHCGALGVYLYPALDAEMISMIFTNGPAVMPAVGGNSPILSTSPIA
                     SGIPSNPPMIIDLSTSAVARGKIASAAKAGGSIPAGWAVNEKGEAITDAKQALMGMLA
                     PLGGAKGFALGLMVESLSAGLSGGSLSRAIPDMFNPDDDKKAQGISHTVITINPASIG
                     KDSKEGLDELAASITASGGRVPGSKRVSPNNLGSGEITLADAVINDLNSWSQKLGLEN
                     IS"
     gene            8425..9393
                     /locus_tag="PLW53_00050"
     CDS             8425..9393
                     /locus_tag="PLW53_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRD35850.1"
                     /translation="MKLTSSNLKHFSVISIFVALAYGISQLQGAISPLFISLLFGLAL
                     VNSGLWGDGGREAANFAAKRCMRIGVVLLGFQISIDKFIEVGPKGLLAVIIIVAVVFL
                     GLRYVAMKSGSSESFSTLIAGGFAICGATAIAAISSTRKSEERDVSYAVALVALCGTL
                     SVFVIPPLANLFSLSDATAGAWIGAAVHDVGQVIATASLMGPAALDSAVIVKLTRVVL
                     LIPLILLLSYKTSEKKSLKSATPVFVIGFVACALIVNALALPESAINLGKESSKIFLS
                     LGLLGMGLSVKWASIKALGAKPLVLGLLAWVVCGGFALAVITSVGL"
     gene            9480..9701
                     /gene="infA"
                     /locus_tag="PLW53_00055"
     CDS             9480..9701
                     /gene="infA"
                     /locus_tag="PLW53_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRD35851.1"
                     /translation="MAKKDGAIEIEGTVAEALPNAMFRVELTNGHKVLAHISGKMRQN
                     YIRILPEDRVIVELSPYDLTRGRIIYRYK"
     gene            9718..9831
                     /gene="rpmJ"
                     /locus_tag="PLW53_00060"
     CDS             9718..9831
                     /gene="rpmJ"
                     /locus_tag="PLW53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRD35852.1"
                     /translation="MKVKPSVKKICDKCKVIRRNGRVMVICDNLRHKQRQG"
     gene            9974..10351
                     /gene="rpsM"
                     /locus_tag="PLW53_00065"
     CDS             9974..10351
                     /gene="rpsM"
                     /locus_tag="PLW53_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRD35853.1"
                     /translation="MARLVGVDLPREKRLEVALQYIFGIGQTRAQATLAATGVSPDVR
                     VKDLQEPDLIKLREFIEANYKIEGDLRREIAGDIRRKVEIQCYQGIRHRKGLPVRGQR
                     THTNARTRKGPRVAIAGKKKETK"
     gene            10351..10779
                     /gene="rpsK"
                     /locus_tag="PLW53_00070"
     CDS             10351..10779
                     /gene="rpsK"
                     /locus_tag="PLW53_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRD35854.1"
                     /translation="MAAPKAKPAATAAAPKAKVKARKKEKKNVALGNAYIKSTFNNTI
                     VTITDPSGAVLAWASSGQVGFKGSRKSTPFAAQLAAESAAKKAQEHGVKKVDVYVKGP
                     GSGRETAIRSLQAAGLEVGAISDVTPLAHNGCRPRKPRRV"
     gene            10797..11423
                     /gene="rpsD"
                     /locus_tag="PLW53_00075"
     CDS             10797..11423
                     /gene="rpsD"
                     /locus_tag="PLW53_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRD35855.1"
                     /translation="MARYAGADCKRCRREKVKLFLKGSKCDGPKCPIESRPYPPGQHG
                     RARAKESEYLLQMREKQKCARIYGVLEKQFRNYYTEASRLAGKTGENLLVLLETRLDN
                     VIYRAGFAKSRDMARQIVRHGHILVNGRKVDIPSFHVSQFDIIDVRTKSQDLTPFIVA
                     SAEFGEKSVPAWLEVVPSAMRIIVHGQPTRAIIDTQVQEQLIVELYSK"
     gene            11547..12545
                     /locus_tag="PLW53_00080"
     CDS             11547..12545
                     /locus_tag="PLW53_00080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRD35856.1"
                     /translation="MLIAQRPVLSEEVVNESRSRFIIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRVAGALHEFTTLEGVKEDLTDIVLNIKNLVLSSENDEPSVAYIRKNGS
                     GVITGADVAVPTGVVVHNPDLHIATLNTKANVEIELTIERGRGYVTAVQNKLVGAEIG
                     RIPVDSIYSPVLRVTYKVEATRVEQRTDFDRLIVDVETKRSMSPRDALASAGKTLVEL
                     FGLAHELNYAAEGIDLGPSIQDAALAADLALPIEDLDLTVRSYNCLKREGIHTVGELL
                     SRSEADLLDIRNFGSKSIDEVKAKLVSMGMQLKDSPVGFDPTQHQNYGVDYSDDQA"
     gene            12557..12907
                     /gene="rplQ"
                     /locus_tag="PLW53_00085"
                     /pseudo
     CDS             12557..12907
                     /gene="rplQ"
                     /locus_tag="PLW53_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            13043..13891
                     /gene="truA"
                     /locus_tag="PLW53_00090"
     CDS             13043..13891
                     /gene="truA"
                     /locus_tag="PLW53_00090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRD35857.1"
                     /translation="MEQPTLKVESGFLRFRIDFSYDGSAFSGWAKQPDQRTIQGEMEV
                     ALSGLVRNKVDLIVAGRTDAGVHASMQVAHFDLPERDKYGKPWNTQDLQYRLNRMLSE
                     EILVRAVSRAPDHFHARFSALRRTYIYKIADGQQHIDPLKRFDITPWYRHLDLSRLNE
                     ASRRLLGDHDFATFCKPGGSGTTLRRLEEFSWQRMADSTLQARVTADAFCYSMVRNLV
                     GAVVCVGESRFEPEWISSLLANKTRVSESMVFPARGLTLIAIEYPADDLLEARSKVTA
                     RRRDEE"
     gene            13892..15493
                     /locus_tag="PLW53_00095"
     CDS             13892..15493
                     /locus_tag="PLW53_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD35858.1"
                     /translation="MGQINVVGIEYALSDGRVLLSDVSFRVGEGSKVALIGPNGSGKT
                     TLFRMIAGDITADAGQIIINGGLGVMRQFIGSIRDESTVRDLLISVSPLRIRQAAEKL
                     KEAEIEMAANNSESQQMAYAQALADWGDAGGYEQEVLWDIAVNNALAKSFDRVSDRLV
                     NSLSGGEQKRLVLEVLLNGPEEVLILDEPDNYLDVPAKRWLEEQIRISKKTILFISHD
                     RELLAATATKVVTLEQGVSGNSAWIHGEGFNTWHDARKARNAQFAQVLERWQDEHKRL
                     KELVLTLQWQAASSPDMASRYRAMQTRLRKFEEIGPPPPPPLEQDVKVNLKSGRTGER
                     VVTCKDLTLENLTEPFTFEIYYQDRVAILGKNGSGKSHFLRRLSGDESVRYSGEFKLG
                     ARVIPGFFAQTHAHPEFVGKTLLEILWQENSLQLGAAKSVLGRYELAQQAEQQFTSLS
                     GGQQARFQVLLLELAGDTLLLLDEPTDNLDLASAEALERALDAYQGTVIAVTHDRWFA
                     KGFTRFLIFGAEGAVYESPEPVWSH"
     gene            15603..16061
                     /gene="rplM"
                     /locus_tag="PLW53_00100"
     CDS             15603..16061
                     /gene="rplM"
                     /locus_tag="PLW53_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRD35859.1"
                     /translation="MRTFSPTPKDVARQWYVIDAADVVLGRLASHAAILLRGKHKPTF
                     APHMDMGDFVIVINAEKIVLTGSKMANKMAYQHSQYPGGLTERSYELLMEKFPTRAVE
                     KAIKGMLPKNSIGKQMASKLKVYAGPNHPHGSQGPIPYVFTQIDQMKVGK"
     gene            16140..16529
                     /gene="rpsI"
                     /locus_tag="PLW53_00105"
     CDS             16140..16529
                     /gene="rpsI"
                     /locus_tag="PLW53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRD35860.1"
                     /translation="MAAGAGIGRRKEAVARVRLTPGTGRWIVNNKNLEVYFPNKVHQQ
                     AVSEPLRTVGAEGKYDVIARIDGGGISGQAGALRLGVARALNEIDTDANRPALKKAKF
                     LTRDARVIERKKYGLKKARKRSQYSKR"
     gene            16547..17872
                     /gene="glmM"
                     /locus_tag="PLW53_00110"
     CDS             16547..17872
                     /gene="glmM"
                     /locus_tag="PLW53_00110"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRD35861.1"
                     /translation="MALFGTDGVRGVAGVDLTAELAFNLSTAAASVLADLGEFAGHRP
                     TAIVGQDSRASGQYLEEAICRGLSSAGIDVYRVGILPTPAISFLVKDRGADLGVMISA
                     SHNPASDNGIKFFDRTGSKLADQIEAKIEGVLGKTVVSNNGAGRVIEDFEAKESYIKN
                     LLKSLDGNLSGIKVVVDCANGAASFVAPDVLKRAGAEVVAISASPNGLNINDNCGSTH
                     LESLIAAVKEEGADIGIAHDGDADRVLAIDDKGSVIDGDFILGILAQDMELPTKTVVG
                     TVMTNLGLIKALEEKGIKFIATAVGDRYVLEEMLAGGHVLGGEQSGHIIMSKYANTGD
                     GLLTALQLISKLKKSGKSASELASFMKRYPQVLINVSGVAKEKLASNQVIAKAVSDVE
                     KKLSDKGRVLLRASGTEALIRVMIEAQDLSLAQELASELATLVKSELSL"
     gene            17887..19713
                     /gene="glmS"
                     /locus_tag="PLW53_00115"
     CDS             17887..19713
                     /gene="glmS"
                     /locus_tag="PLW53_00115"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRD35862.1"
                     /translation="MCGIIGYTGASPALSMVIDSLRKLEYRGYDSAGIALTGKAGEKL
                     IVAKRAGKLSNLESALTSDHADSLSGIGHTRWATHGGPTDENAHPHISSDGKVAVIHN
                     GIIENYLELKVELEKRGHKFASQTDTESVAHLLSDEFNLSKDLADAMRKVCKRLRGSF
                     TLLAISSDSPEVIVGARRNSPLVVGLGKGENFLASDVSAFIAHTKSALELGQDQVVQV
                     SPDSVLVTDLDGKQVSAKPFEITWDAKAAERGGYTHFMLKEIFEQPKAISETLLGRFS
                     DDSKVICSELSSLNLDIDRILILACGTAYHAGLIGKYAIEQWSKIAVDVELASEFRYR
                     KPFLTPKTLVITISQSGETMDTLMAARHAKDLGAATLSICNTMGSSIARESDAVFYTH
                     AGPEIAVASTKALLTQIVALKVIALYLAQNNKSLSGAQIAAIISELLALPNKVEEILE
                     TVEPLREMTRGFKDATSVLFLGRNVGYPAALEGALKLKELAYMHAEGFAGGELKHGPI
                     ALIEEGTPVIAILPPASSPIAEKMLSNIQEVRARGANVIAIADSSISLDVKNIIRIPS
                     CDELLQSILAIVPLQVFAYEMAVARGNDVDQPRNLAKSVTVE"
     gene            19710..20414
                     /locus_tag="PLW53_00120"
     CDS             19710..20414
                     /locus_tag="PLW53_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRD35863.1"
                     /translation="MSGPSSLFDDVVDQRIEIRRREMNIALTWCAVLLLGFALVTLDV
                     INKGLLYRFDQFVADLSRPKLSGFTNFLVLRIDDLGLRWITALILLITAGLISRRFKS
                     FRPLNLAFLSLVFLNIFVGASKIFFGRCKAKEDFDLCMFSDGMAYPSGHISNALVTWG
                     LFAYIIFRYTHRAPFEGLKLYWLVAVITIAVGAVSLIRNTHWFTDLLGGMFLGGAILV
                     LVVAIDRFIPSTRQPS"
     gene            20442..21584
                     /gene="alr"
                     /locus_tag="PLW53_00125"
     CDS             20442..21584
                     /gene="alr"
                     /locus_tag="PLW53_00125"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRD35864.1"
                     /translation="MENRAEVLIDLGAIRDNIKYLMAKSGKPALAVVKADGYGHGLLP
                     VAKSALSAGASWLGVALLEEARALRQGGISAPIIAWLTPITDDFKSAINEDIDIAIAS
                     LAHLEAITSASKSLGKCARVHLEVDTGMSRGGALGEWSDLVRSAKAYQDSGLIEVIGI
                     WSHFARADEAGHEFNKKQLENFKSAVAQAKSVGITPQVIHLSNSAAAINDVDSHFDLL
                     RLGIAIYGLSPDFSHMGSSEKLNLKPAMSIRARIHLVKDVPAGSQVGYGGSAITKVDT
                     KLGVIAMGYSDGIPRNANSEAGVLVGNKRAPIIGRVSMDQFVVDLGRDSSAKAGDWAY
                     IIGSNPLDSYTSASSYTADSWASASATINYEIVTRIAARVKRIYQG"
     gene            21595..22368
                     /locus_tag="PLW53_00130"
     CDS             21595..22368
                     /locus_tag="PLW53_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD35865.1"
                     /translation="MTEGVVLSVNDLSVNFGGLKALNGVNFELRKGEVLGLIGPNGAG
                     KTTVFNALCGIVTPDSGSLSLNGKEIKWPKPHKLAKLKIARTLQGVGLFPDLTVYDNV
                     MIGANKFSETGLLRAAFGTNHKDEKRLKERAQAALERVYVGAVADRRADTLAYPDTKR
                     VSIARALVAEPEILLLDEPAGGLGPQDIEWLNSLINNLKSRTSIIIVEHHMDVVMSVC
                     DRIYVLNFGEIIASGTPAEVRDNEAVIQAYLGTKSKAKS"
     gene            22365..23078
                     /locus_tag="PLW53_00135"
     CDS             22365..23078
                     /locus_tag="PLW53_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD35866.1"
                     /translation="MSGLVVNRLSVSHGAIEALRAVSFSVEVGQLAAIIGSNGAGKTT
                     LLRALSGLNTQTSGSAQWNEREILGARPDALVRRGISHVSEGKSVIAELSVKENLILG
                     GIWRNKKSDTSAAIAEVVELFPVLGQRMSQSADSLSGGERQMLAIGRALVSRPKLLLL
                     DEPSLGLAPLVIEQIFATIDQLRRKLGLTVVLVEQNAMSALEIADIGIIINLGEVVAS
                     GKANELIDDPALRAAYLGY"
     gene            23078..23974
                     /locus_tag="PLW53_00140"
     CDS             23078..23974
                     /locus_tag="PLW53_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD35867.1"
                     /translation="MQKVIETLLIGLSTGSIYALMSLALVLVWRSTRVVNFAQAGQAM
                     LSTYIGFEIASRSEIFWLGLVFAIAAGALVGAGVDYFFMRVLFKHATSGPAAVVAPVI
                     ATLGLLGLIRSIVGLTWGGEFKSIEAPASTDGFTVGDGTIPFSNLDVLIIATVSIILI
                     ALAYIFQRTNLGLALRASAYAPEIAQLAGIRTARTRTIGWSIAGAVGGVAGMLVTSNN
                     FLSPYSLDLLLVFGFIAAVIGGLESMVGSVIGALVLGIGLTFILNYVGTSLVFIGGFS
                     VLILVLLIKPDGIMAKGKARRA"
     gene            23967..24938
                     /locus_tag="PLW53_00145"
     CDS             23967..24938
                     /locus_tag="PLW53_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD35868.1"
                     /translation="MRKKIAIFVLIGWVLLIIGDRVDELRVYQGAQMAMYTIALASIV
                     LLTGYSGQVSLGNGAMMAVGGYGAALTLNNLGTPIWFAFLMAIVTASLFGALLGAAAA
                     RVSGPYLAGTTLALAVGLPAIANQFAFLGGEPGLLFDVGFPPERFGDEFTQYKWFFWI
                     ASFIALIALFITSNILSSRFGRTWRAIRGNSLAAELCGVHSARMKVLAFTVSSGLAGL
                     AGAVLAMTISLVAPGAYGLALSFALVTGAVLAGISSLPGVVLGAFILVVIPEISVALA
                     DRLGDADKLNNYLPGLIVSVLLILSVIFTPNGPGENLRKHRHKKPAH"
     gene            25004..26299
                     /locus_tag="PLW53_00150"
     CDS             25004..26299
                     /locus_tag="PLW53_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD35869.1"
                     /translation="MIRKTRIKRTWLAAAVIASGSLLATALPANAETGVSASEIKLGI
                     TLPLTGAASPGYNKIGNAINAYFKYVNDNGGVYGRKVTLIQKNDEYSPQLSIAKTNEL
                     ILRDKVFALVGPLGTANFTAVHKSVGLAKRGVPSLFLNTGFSGFSNKKSYPSSFMILP
                     SYAMEAKIIAQFLKENHAGKKLGIVYQNDEFGIDGLGAFKAAGTKFDVSIPYTSGTQS
                     AATAQTWVSQLAAGGAQVVVFFGVTSATAPLLAVAAAAKYAPQWILGSVGSDPLTLRA
                     LGVPVAVLNGAQTPAGNPSPNDTSDEYVKQFQEINTKYNTGVAFDDYVLQGMNIGLMT
                     VQALRAAGKNLTRKGLIRAMETKGSTFASVAYSPLGFSRTSNVGHTGYYMAVMDANGD
                     RKPFGGKVTLYTTDSGSGPVVVSTFKRPAMPAKGLPSNS"
     gene            26296..27921
                     /locus_tag="PLW53_00155"
     CDS             26296..27921
                     /locus_tag="PLW53_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35870.1"
                     /translation="MRTFKFKRSLTLVAVSSLLSITVVSIPTAAQANPVCTTASGVTT
                     CLGATSDTAPYMLMTPANFNGTVYLYSHGYRPNVPVPAGIPGYGGYTVTNTPEPAPGG
                     NAQIIGTLLAKGYAVMGSGFARQGWNADSAIKTNVELIGVFKKQYPKTTKVVAWGNSL
                     GGFITQALAEKYPDLISAAAPLCMASDLTPLMTMAGDALWGIKTFFDPTIKGGGYSAG
                     TAGYAEAMGDLVKVFTVIGAMQAAIAANPSAPAWPATSKVPDALKAVPSRSALVLIAL
                     MSGVPMQSAHFDSTSAPAGLPASSALSFQLAINPALAALENLANAAALAVLATHDVEQ
                     QAGGAVFDNTATDYSARVSAEQFIWSSALSGNAGVGALLGYLAAVPRVKGNPAAIAKM
                     ATLTTHSGKVEIPTILFTGTADPITTAGNQQSVIDKYAAYSAEKWAAAKKEGTRKKPA
                     NNQLVLWNFPPEKYTKFTATGSPDTTVAAATGTNHCNFTVSQYLAIADLLAYAADNGK
                     NMSGGALYTKIRKAGNMTYDRGYSAPKLKYYGE"
     gene            28061..29362
                     /locus_tag="PLW53_00160"
     CDS             28061..29362
                     /locus_tag="PLW53_00160"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRD35871.1"
                     /translation="MASLAERRRIARLVHRFGFGPRPGEFSNLLANGFDQAAEKYLNA
                     PSQDAFADTQDEPMVRDQGKRPAPNSPDVVAYATEKRAQLTSLSFWWLDRMVLSEHSL
                     RERMTWFWHGHWATSYQKVDDALPMYLQNQTLRRNALGNFAQMSREMVNDAALIFWLD
                     GQSNTVKAPNENLSRELMELFTLGVNRYSEDDVKETAKALTGYKVDKSSGKVIFYPKQ
                     HFAGSIRVLGTQGSFDASSLSDFLVARSDCALFITERIWYRFISSMNPLADTTLTSSF
                     ASRDIAALVKAIGRHSALDNPDNSMVKSPIDWFVSAARALSITPSKFSNPNTIRNYLD
                     LLGQRPFFPPNVGGWPADQAWLSTSSAQYRIQFATQLVKEADLTPISSLAPQARIDGL
                     ADWLGVVEWSSRTKMALNGAIKDPARLALLALCSPEYVVSA"
     gene            29372..30598
                     /locus_tag="PLW53_00165"
     CDS             29372..30598
                     /locus_tag="PLW53_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRD35872.1"
                     /translation="MDTITRRQFLKATGAGAIGAAAMSLSIEEIANAAITRPLPLGTP
                     ILVVVTLYGGNDGLNTVVPVNDPIYQSLRPGIAYKPEDVLPLGENLYLNGSMSGMHSL
                     WNKNQVAIVRGVGYPNSDRSHFSSMAIWQSAIIGAAKTGWLGRWVESQPEDPFLAISL
                     GSILPPLLAGSKRSGSVLPLGGLKVPNGSLASDCIKLAIESRSDNLLQAMAAKTMRNL
                     FTVSSDITPILKSPAPAAPDLPTAIGGNAGGDTNLSQQLDIVAKLVSAGAPTRVWSVS
                     LGGFDTHADEKGSQAILLGVVSQGISKFMSQMRATNRAKDVVVLVYSEFGRRVRGNAS
                     DGTDHGTSGPVFLLGERVKGGFYGEQPSLNNLKNDDLAVTTDFRDIYASVLEKVLSTP
                     AEKILGNWKGRTPLFN"
     gene            30615..31058
                     /gene="tsaE"
                     /locus_tag="PLW53_00170"
     CDS             30615..31058
                     /gene="tsaE"
                     /locus_tag="PLW53_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRD35873.1"
                     /translation="MLIQSPEQMRDFGISLSKKFRAGDVIVLKGDLGAGKTALASGIG
                     MGLGIEGVSSPTFIISRVHKAKVPLIHIDAYRLLLSGAKGFEFDDLDIATSKDHAITV
                     IEWGEGLVNRLSDEYLVIDIAFGTDENERLLSATSIGPRWAGFSL"
     gene            31055..31609
                     /gene="tsaB"
                     /locus_tag="PLW53_00175"
     CDS             31055..31609
                     /gene="tsaB"
                     /locus_tag="PLW53_00175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRD35874.1"
                     /translation="MNYLVIDTATDRSSLALFSNNELIYSGFHDGATEHAEALPKLLK
                     ECLSVESRVDQVVVGMGPGPYTGLRVGIIFAMSFANARQIPWIGVCSLDGIDIDSDSY
                     LVATDARRKEIYFARYQDGIRTQGPSVSAPSEIKDLDVKKFGFGFGDPLYPSMALLLA
                     KSKTQDVKQPFYLRRPDAIESSAR"
     gene            31606..32058
                     /gene="rimI"
                     /locus_tag="PLW53_00180"
     CDS             31606..32058
                     /gene="rimI"
                     /locus_tag="PLW53_00180"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRD35875.1"
                     /translation="MKATFRQMISLDIPLLVSMEREIYPESPWSANQFKEELAGMPKS
                     REYLVALDEIEVVGYGGVALLGDVADIHTLTIKQSHRRLGIASSMLEKLETWAIQRGA
                     KALMLEMREGNEAAMSLYQKAGYQLISRRDNYYAKGIHALIMRKEVEQ"
     gene            32059..33093
                     /gene="tsaD"
                     /locus_tag="PLW53_00185"
     CDS             32059..33093
                     /gene="tsaD"
                     /locus_tag="PLW53_00185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRD35876.1"
                     /translation="MSEPLVLGIESSCDETAIGIVRGRKLLANVISSSVDEHARFGGV
                     VPEVASRAHLEAMQPAITQALNESGVKLKDLDGIAVTAGPGLVGTLLLGVSTANALSL
                     ALDKPLYGVNHLAAHIAVDLLTDDRKISPAIALLVSGGHSSILQVNDLTKDIKPLGRT
                     LDDAAGEAFDKVARLLNLGFPGGPLIDRDAKDGRGDAINFPRGLNQAKDMAEHRFDFS
                     FSGLKTAVSRYLVANPSYNRSDVSASFQEAVVDVLLDKAVKACLSSGIQTLIIAGGVG
                     ANSRLRSLAASRCEKAGIELRRPPANLCTDNGAMVAALGSLAISSGIEPSKLGIQVAS
                     EISPSVAIWA"
     gene            33211..33504
                     /gene="groES"
                     /locus_tag="PLW53_00190"
     CDS             33211..33504
                     /gene="groES"
                     /locus_tag="PLW53_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRD35877.1"
                     /translation="MAIAIKPLEDRIVIKINEAEEKTASGLVIPDTAKEKPQEGTVVA
                     VGPGRFDDGVRVPMDIKVGDVVLYSKYGGTEIKHGGEEYLVLSARDILAVIAK"
     gene            33532..35166
                     /gene="groL"
                     /locus_tag="PLW53_00195"
     CDS             33532..35166
                     /gene="groL"
                     /locus_tag="PLW53_00195"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRD35878.1"
                     /translation="MGKSLQFDENARRAMERGVNILADTVKVTLGPKGRNVVIGKSFG
                     APLITNDGVTIAKEIELSDPSENMGAQLVKQVAEKTNDVAGDGTTTATVLAQAMVREG
                     LRNLAAGAQPMGIKAGIEAAVAAVVEKLRANSTKISAKEQIADVATISAQDRSIGDLI
                     AEAMDKVGKDGVITVEESSTTGLELEFTEGMQFDKGYISPYFVTDQDRMEATLEDAYI
                     LISAGKISALADLLPILEQVAKASKPLLIIAEDVEGEALSTLVVNRMRGVFTSAAVKA
                     PGFGDRRKAMLQDIAVLTGGQVISSEVGLKLDQISIDMLGKARRIVITKDNTTIVDGA
                     GNKKDVEGRIAELRREIEAADSDWDREKLQERLAKLSGGVCVIKVGAHTEVELKEKKH
                     RLEDAISATRAAVEEGIVVGGGAALVHAATVLDGDLGHEGDSAVGVRLVAKACQEPLR
                     WIAENAGQEGYVVVAKVRTLKPNEGFNAATDEYGDLVKAGVIDPVKVTRSALANAASI
                     AAMFITTEALVFETPEDKKEEASGHGHSHGPGGHSH"
     gene            35241..36167
                     /locus_tag="PLW53_00200"
     CDS             35241..36167
                     /locus_tag="PLW53_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35879.1"
                     /translation="MVSESLNISTHIWAITLGVIFLVTLGDLIWAWFRRNTITTLKEA
                     AIWTGIYVSAAIAFGISLRSWGGQTKSAEFFAGWITEYSLSIDNLFVFLIILSRLKIE
                     KEKEQLVLLLGILMALVMRGIFIVIGAAVVERFVAVFFFFGAILIWTAYKLITEDPEE
                     DEWQENRVITALRKRGYSTFTIALVALGTTDLLFALDSIPAIFGLTRDPYIVFMANAF
                     ALMGLRQLYFLVGILLKRLVYLSKGLSIVLGFIGIKLIIEALHGVGIDYIYGVKVPHV
                     SLTVSLGVIISALAVTTVISLRASGNFEKGRE"
     gene            complement(36177..36479)
                     /locus_tag="PLW53_00205"
     CDS             complement(36177..36479)
                     /locus_tag="PLW53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRD35880.1"
                     /translation="MSETSRLPKPVIDNWQWQFEGACRSLPTEMFFHPDGERGPRRRA
                     RENAAKAVCASCPVLAACRAHALASQEPYGIWGGLTEDERLEIISRASRQSSPYSA"
     gene            36580..37509
                     /locus_tag="PLW53_00210"
     CDS             36580..37509
                     /locus_tag="PLW53_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD35881.1"
                     /translation="MAQRPKSANLKAGLEDERLTVASVARRLGIAPATLRTWDRRYGL
                     GPSSHVEGEHRRYSAKDLARLTLMRKLIIQGYSPCDAAEVALKSRTKSFESLASREVA
                     VEVDDCSDLIESLLKAARVYDRSFIEDVLRQQIEKRGIINTWNELIVPVLIAVGNEWA
                     ELGTGIEIEHLLTEIITRLMQSSNQTPVNPINPRPVLLACIGEEMHTLALYTLGAALA
                     ERNIQSQFLGARTPISAVGAMVKKSAPPAVFLWAQLAKNGKSEVVGDIPRVRPAPRII
                     LGGPGWRKTSKKSLVKVDCLDSAITEITRAVGA"
     gene            37535..38656
                     /locus_tag="PLW53_00215"
     CDS             37535..38656
                     /locus_tag="PLW53_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRD35882.1"
                     /translation="MSESVNEKVAMLGLTYDDVLLLPDASEVVPSEVETKTFLTRSIQ
                     LDIPLISSAMDTVTESAMAIAMAKAGGIGIIHRNLAIDEQVTHVKLVKGANLRVGAAV
                     GVGEDGFERAEALIGAGVDVIVVDTAHGHHRAVLDAIVRIKKHFPRQEVIGGNVATRA
                     GAQALINAGADAVKVGVGPGSICTTRVVAGVGVPQITAIMEAAKACQKADIPLIADGG
                     LQYSGDIAKAIVSGADTVMLGSLLAGCDESPGELVESDGRKFKIYRGMGSLGAMQSRG
                     EQKSFSKDRYMQDDVLAEDKLVPEGIEGKVAYRGSVAQVVHQLVGGLRSAMGYAGAPD
                     IAALQKRGRLIQITAAGLSESHPHDVLDVADAPNYYRSK"
     gene            38656..39768
                     /locus_tag="PLW53_00220"
     CDS             38656..39768
                     /locus_tag="PLW53_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HRD35883.1"
                     /translation="MVDIEISPGKRARTAYSFDDISIAPSRRTREPSEVSINWQIDAY
                     SFGLPLLAAPMDSVVSPESAIAIGKLGGLGVLNLEGLWSRYENPKIQLGEISSIPAEQ
                     ATRRMQELYQAPIRAELIKERLAEIRAAGVCVAAALSPQRTAQFHKTVIDAGVDIFVI
                     RGTTVSAEHVSKTNEALNLKKFIYELDVPVIVGGCATSQSALHLMRAGAAGVLVGFGG
                     GAAHTTRQVLGISVPMATAVADVAAARREYLDESGGRYVHVIADGSVGKSGDIAKAIA
                     CGADAVMLGSPLARAESAPGRGWHWGPEAHHNELPRGTRVYVGSSGSLEEILLGPSHS
                     ADGSMNLFGALRRAMATSGYSDLKEFQRVEIVLNRA"
     gene            39782..41332
                     /gene="guaA"
                     /locus_tag="PLW53_00225"
     CDS             39782..41332
                     /gene="guaA"
                     /locus_tag="PLW53_00225"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRD35884.1"
                     /translation="MQSNNHVLVVDFGAQYAQLIARRVRQAGVYSEIISHKCSLEEIL
                     ARNPKAIILSGGPSSVYVDDAPLIDRALFEQNIPIFGICYGFQVMAQLLGGEVAKTGL
                     SEYGRTAFNCDTSAKLFSGLDSSFNVWMSHGDSVTKAPEGFQATGSSASTSVAAFESS
                     DGKLAGVQFHPEVLHSENGQEILRKWLIDIVKCKPTWTSENIVENEIINIKSQVADKR
                     VICGLSGGVDSAVAAALVQKAIGKQLTCVFVDHGLLRQGEAEQVEKDFVAATGVELMV
                     VDAKKRFLDALKGVKDPEEKRKIIGREFIRVFEDAAKKLSADSKIEFLVQGTLYPDVV
                     ESGGGSGTANIKSHHNVGGLPDDLDFKLIEPLRTLFKDEVRQVGLSLGLPDQIIWRQP
                     FPGPGLAIRIVGEVTEERLRILRQADLIAREELHLAGLDQMIWQSPVVLLAEVRSVGV
                     QGDGRSYGHPIVLRPVSSEDAMTADWSRVPYEVLAKISNRITNEVSEVNRVVLDITSK
                     PPGTIEWE"
     gene            41338..42015
                     /locus_tag="PLW53_00230"
     CDS             41338..42015
                     /locus_tag="PLW53_00230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD35885.1"
                     /translation="MRSKRKVTVIALLSAVLLIPIAPVSAESKEINVAAPAKKVKCKV
                     SKASAQSPMRLPAPENVMKRAPKYLTLQTNCGNIVIETLAKAAPVTITVMSLLARGGY
                     FDRTLCHRLTTEGIFVLQCGDPTATGSGGPTFRYRDENLPLASENNYPEGSVAMANSG
                     PNTNGSQFFLVYKDTTLGPNYTLWGKIVSGLEIVKYIAQGGVKNGGVDGAPLRTIGIE
                     RAITSNS"
     gene            complement(42012..42734)
                     /locus_tag="PLW53_00235"
     CDS             complement(42012..42734)
                     /locus_tag="PLW53_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRD35886.1"
                     /translation="MTPIADEEIKRILVVMAHPDDCDFGAGGSIAKWREAGIQVSYCI
                     ITNGDQGGEESDVPVEEMAKVRQREQRAAGAALGVSEITFLNYRDGSLIPTIELRRDI
                     VKAIRIAKPDRMVVQSPERNWERIYSSHPDHLAAGESAIQAIYPDARNPYAFPELKAE
                     GIEPWRVREVFITGSPTPNHFIDITETIDKKIAALKAHVSQTAHNPELESMVRQWGER
                     NAEANGLAAGRVAEIFKVVSTD"
     gene            42758..44986
                     /gene="pcrA"
                     /locus_tag="PLW53_00240"
     CDS             42758..44986
                     /gene="pcrA"
                     /locus_tag="PLW53_00240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HRD35887.1"
                     /translation="MAALNEDKLLAGLNPQQAAAVTHSGSPLLVVAGAGSGKTRVLTR
                     RIAYLMAARGVDPHEILAITFTNKAAGEMKERVAQLVGNRAKIIWVSTFHSASVRILR
                     QEAGRLGYKSTFSIYDQADSLRLITLVMRDLNLDPKRYNPRGVASVISGAKNDLLGPS
                     DYRNATQNSFEEVVAEIYAIYQARLTSANAMDFDDLIMKTVDLLQKFPEARSRYRHRF
                     KHILVDEYQDTNHAQYILIRELVGSDREGIPAAELCVVGDADQSIYGFRGATIRNIMA
                     FEEDYPDAQTILLEQNYRSTQNILSAANAVISLNEARKEKNLWSDAGSGAKLVGFVGE
                     NEHHEAEYIRDELFRLEREGISNFGQSAIFYRTNAQSRVFEEVFMRATIPYKVVGGVR
                     FYERKEVKDLLAYLRVLVNPDDEVSMRRIINTPKRGLGDRALEAIDDYAKTEGITFWQ
                     GLNRASAAGLGAKASSAVAEFVAMIAALQVLVEANRPPAIIAAAVLEQSGLLDELRDS
                     HDPQDEVRIENLEELVAVAEEYQEREVDEGQEISLAGFLEDVALVADADEIPEGEDHG
                     GVVTLMTLHTAKGLEFPTVFLTGMEEGIFPHSRTLGEKKELEEERRLAYVGLTRARER
                     LYLSRAEYRSSWGAPNYNPPSRFLDEIPDALIDWNKSESGYISSPMTRRKYAPSALPK
                     AAIKSNTLQLNIGDRVSHDTFGLGTVIALSGEAEKAEATINFGSYGDKRLLLRYAPVD
                     KL"
     gene            44990..46153
                     /gene="sucC"
                     /locus_tag="PLW53_00245"
     CDS             44990..46153
                     /gene="sucC"
                     /locus_tag="PLW53_00245"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRD35888.1"
                     /translation="MDLFEYQARDLFEANSIPVLKAKVATTAAQARDAAQEIGGKVVV
                     KAQVKIGGRGKAGGVKLAENGEDAYVKAEAILGMDIKGHIVKKVMIAAAAPIESEYYL
                     AILLDRSNRSFLVMASVAGGMDIEEVAHKTPEKLAKVHIDPNEGISQEKALAIVRQGG
                     FGSDVENQVADVLVTLWKTFQSSDATLVEVNPLVKTVDGRVIALDGKVTLDDNAAFRH
                     PEFEALIDHEAADPLEALAKERDLNYVKLQGEVGVIGNGAGLVMSTLDVVAYAGEKFG
                     GVKPANFLDIGGGASAQVMADGLSIILGDKDVKSVFVNVFGGITSCDAVANGIIQALE
                     MLGDKASKPIVVRLDGNNVIEGRAILNKANHPLIQQVESMDGAAARAAELAAK"
     gene            46153..47034
                     /gene="sucD"
                     /locus_tag="PLW53_00250"
     CDS             46153..47034
                     /gene="sucD"
                     /locus_tag="PLW53_00250"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRD35889.1"
                     /translation="MAIFLTEKTKVIVQGITGSEGSKHTRRMILSGTNIVGGVTPGKG
                     GQVVEIEGKSLPVFNSVKEAIAATGADASVIFVPPKFAKSAVIDAIDAHLPLIVVITE
                     GIPVHDTASFYAHSLKVGKSRIIGPNCPGLISPGKSNIGITPADITGPGSIGLVSKSG
                     TLTYQMMYELRDIGFTTAVGIGGDPIVGTTHIDCLRAFQDDPETKAIVMIGEIGGDAE
                     ERAAAFIKEYVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAEAKKVALEAAGVKV
                     GKTPSQTAELLRAILKG"
     gene            47049..48188
                     /locus_tag="PLW53_00255"
     CDS             47049..48188
                     /locus_tag="PLW53_00255"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HRD35890.1"
                     /translation="MLPLLKVAFGQGVRGITLILLPLAFISLITWATAGSSTGNTADP
                     MRAAIWFFLIAHHIPLDLSLSNETISGRLTFFPIGALIIPFLVIKNSLLRMSERLGTN
                     RSSDKRAQILALSFAYSLLGTLLSLASLGSTVKAPFYIVFPILCIVSAVSGFIASNLL
                     PDHGIQFPWQRALKLATLLLLALIGFAGLVFSFSLIWHFDTVLDLTRVIEPGVFGGLA
                     FLFIQILYLPNFFISTLSYIAGSGVVIGNGTWLNPFVHRLDEIPAISLLGGLPVGAQP
                     LFILLTLVIVGLGIVMGKYGVSKYQDQVELRRFFLALFGFIAGLTLFIARASSGELLS
                     PNLESVGPHWYLMPLALSLEIGLGVAIFLYTPVVISKVRNFRAAK"
     gene            48218..49054
                     /locus_tag="PLW53_00260"
     CDS             48218..49054
                     /locus_tag="PLW53_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRD35891.1"
                     /translation="MAQAKVLDGVAAAKAIRQEVALKVAKLKNRPGLATVLVGEDPGS
                     RSYVDGKHRDCSQVGINSIRIELPQTVSQTELLSEINNLNSSKDCSGFIVQLPLPAHI
                     DTNKVLSAIDPDKDADGLHPINLGKLVLSQGGVIPCTPRAIIELLGRNEISLSGKEVV
                     IIGRGTTVGRPLSLLLSSKGIDATVTIVHSKSSDIKSHTKRADVVIAALGKAHFLTAD
                     MVKEGVVAIDVGITRTSEGLVGDIDPEVMKIASAFAPMPGGVGPMTRAMLLSNLLALH
                     KD"
     gene            49062..49274
                     /locus_tag="PLW53_00265"
     CDS             49062..49274
                     /locus_tag="PLW53_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35892.1"
                     /translation="MNIKQISYALALSGVLTGALLSVRIGALIIAAGFILFLSRDLAS
                     MRPIQKVIPIALVIALIAIALALPRG"
     gene            49292..50248
                     /locus_tag="PLW53_00270"
     CDS             49292..50248
                     /locus_tag="PLW53_00270"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRD35893.1"
                     /translation="MSRSGKVTVVGAGFYGSTTALRLAEYDIFESVVLTDILDGKPEG
                     LALDINQSRSIEGFESRVIGATTTADGKGYEATAGSDVVIITAGLPRKPGMSRMDLIG
                     VNAGIVRGVAENIAKHSPNAVVIVVSNPLDEMTALAQIATKFPYQRVMGQAGMLDTAR
                     FTHFVAEKLNVPVASVKTLTLGSHGETMVPIPSRCTVDGAALSTKLSDAEIQELVTRT
                     RNGGAEVVALLKTGSAYYAPSAAAARMAKAVIQDSGEVMPVCAWVDGQYSITGVYLGV
                     EAQIGRAGITKVVESDLTASELAELKVAAEAVRAKQADVKDL"
     gene            50281..51495
                     /locus_tag="PLW53_00275"
     CDS             50281..51495
                     /locus_tag="PLW53_00275"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRD35894.1"
                     /translation="MSKIKVQGSVVELDGDEMTRIIWDFIKKQLILPYLDINLEYYDL
                     GIQHRDATDDQVTIDSAKAIQKHGVGVKCATITPDEARVKEFSLKKMWKSPNGTIRNI
                     LGGVIFREPIIIKNVPRLVPHWSKPIVIGRHAYGDQYRATDFRVPGPGKLTVTFTPED
                     GSAPMEFNVFDFPSSGVAMAMYNLDDSIRDFARASFNYGIARKYPVYLSTKNTILKAY
                     DGRFKDIFAEVFEKEFKAEFDKLGLEYDHRLIDDMVATSLRWEGGYIWACKNYDGDVQ
                     SDTVAQGYGSLGLMTSVLMSPDGRTVEAEAAHGTVTRHYRDHQAGKPTSTNPIASIFA
                     WTQGLQHRAKLDNTPELGKFASSLERVCIETVESGKMTKDLALLISSDAPWLNTQEFL
                     AAIDENLQKEMK"
     gene            complement(51553..52635)
                     /gene="ugpC"
                     /locus_tag="PLW53_00280"
     CDS             complement(51553..52635)
                     /gene="ugpC"
                     /locus_tag="PLW53_00280"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRD35895.1"
                     /translation="MASVVFKDASRIYPGTSAPAVDKLNLTVNDGEFLVLVGPSGCGK
                     STSLRMLAGLEEIDNGAIYIGDREVTNVAPKDRDIAMVFQSYALYPHMTVAENMGFAL
                     KIAGVAKEERERRVLDAAKLLDLEPYLERKPKALSGGQRQRVAMGRAIVREPQVFLMD
                     EPLSNLDAKLRVATRTQIAALQRRLGITTVYVTHDQVEAMTMGDRVAVLKDGLLQQVD
                     TPRNLYDNPANAFVAGFIGSPAMNLLTVPVSGGKAKLGNLDVDVPSSAGASVTVGIRP
                     EGFSSSSKGFDVVVEVVEELGADAFVYGKPADSNVKFANASDEGAQIILRWDPKNPPK
                     PGQTVTVTAKAEAVHLFSTADGKRIK"
     gene            52806..54131
                     /locus_tag="PLW53_00285"
     CDS             52806..54131
                     /locus_tag="PLW53_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRD35896.1"
                     /translation="MDPGGANPFTLQDLALDISVVAFFIALGALFVAAEIALISLREG
                     QLKALAEKSRRGAKVADLASHPNRFLAAVQVGVTFSGFLSAAFGADRLGDYLIPALED
                     AGLSNSISETISLVGLTLVIAYFSLVFGELVPKRVALHSSEKIALFVVTPVSVTARLF
                     RPLIWLLSHSSDLVLKLFGIDPKASREDISEEELVDLVTGHKALTEEERDIVEEVFNA
                     SERLVHEVMVPRTEVDFLDANLLVSDAILMAVEKAHSRYPVVRGSSDEVIGFIHVRDL
                     LDPEHSGKGIAIMNLVRSIMFLPGTKGVLPALSEMRAARQQIAIVLDEYGGTDGIVTL
                     EDLVETLIGDIHDEYDGHEVEVSNQSRTGDYEIDALISLEDLAEQTGIEIPEGPYETL
                     SGYVMHELGRIPQLHDVIHLAGMRLTVLSLEGKRVGTVLISKLHTQDKN"
     gene            54144..55154
                     /gene="trpS"
                     /locus_tag="PLW53_00290"
     CDS             54144..55154
                     /gene="trpS"
                     /locus_tag="PLW53_00290"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRD35897.1"
                     /translation="MKRVLSGIQPTADSFHLGNYLGAVKQWVELQSENDAFYCVVDLH
                     ALTVETDPQVLRKRTLLAAAQLIALGLDPDKCTLFIQSHVPEHNQLGWVLECMAGFGE
                     ANRMTQFKDKSQKSGADRTVVGLFTYPILQAADIMLYQANRVPVGEDQRQHIELTRDL
                     GQRFNSRYGQTFTIPEAHIIKSAAKINDLQDPNAKMSKSSASNSGVIDILDSAEVNAK
                     KIKSAVTDAGKEIKFDEKEKPGISNLLTIHSALSNRAISDIENEFAGKGYGDFKSAVA
                     EVVTSYFAPVKKRTEELLSDEAHLLKILEQGRQKARAVAAETLAKTYSALGLVPSSFK
                     DK"
     gene            55158..56114
                     /locus_tag="PLW53_00295"
     CDS             55158..56114
                     /locus_tag="PLW53_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD35898.1"
                     /translation="MRSRALLIALTISLFSPFTTAIAAEPKVAIAYDIGFLGDNSFND
                     AVHLALKAAKKKYGLVEPFVREVPTNGTAVDRLTRLRFLAKSGYTLIIAVGPGYRQTV
                     ARVSKEYTETQFAIINDKSIAQLNVSNIYFREDHGAYLAGIAAANLSKRKSIAFIGSE
                     PELLESFTKGARVNFPKVKIKNVSYPDSVESLRSQLSGVDIAYSTWDGDPSVVAVISE
                     DFAGKIKLISEIPDQYFAQIPQTKSIVVATVSKQMSKPISQLVSAALESRAIIDVLDD
                     AEGIYGREYNATNGGVGITFNISVNSSLKSQLRQALIQLKQG"
     gene            complement(56115..56801)
                     /locus_tag="PLW53_00300"
     CDS             complement(56115..56801)
                     /locus_tag="PLW53_00300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD35899.1"
                     /translation="MSKQKSILITGASGLIGSALCKTFIAAGWRVVGQVNSSKGIDGI
                     EVFRKDLSKASSGTELVEQIGAIDCVINNAADQSILDLKHYSSTKAQEMFQVNLLSPI
                     EIILAAKSKGATLAINISSIEALNSRSGHEIYGASKSALESVTRSLALSLAPMRINGI
                     RLGLIGDDKLESRWPEGFSSWTNTVPAGRIGSAEEVAELALAITSETFSFASGSIIDF
                     DGGKSAHPGW"
     gene            complement(56794..57219)
                     /locus_tag="PLW53_00305"
     CDS             complement(56794..57219)
                     /locus_tag="PLW53_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD35900.1"
                     /translation="MNKDELIEKLDLQPLEGEGGLYSTIYRDELSNAIYFMVVSPDFS
                     AWHRLPQAELWLHLSGDPLLLHSIENGQLVSHRLEQEGQHLAYQVPAKTWMAAESLGS
                     YSLVSCFLAPAFSTMELAGRDELISQFPNIANLPELFHE"
     gene            complement(57206..58528)
                     /locus_tag="PLW53_00310"
     CDS             complement(57206..58528)
                     /locus_tag="PLW53_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRD35901.1"
                     /translation="MSDFDLVIENATYIVTSDSKNTVLKDCAIGIKDKKIVAIQGAGS
                     LKGANTYNAKGKLIAPGLINTHTHLAMSLLRGWAEGVNLQGFLERVWAAEGLIMNPKN
                     VALGTQLGALESLLAGTTTTLDMYLYPDAAHKGAASTGIRHITGPLFFDFTGLDGLDW
                     EARLEIAKKWEQVLQDVGGPDVPPYLMPHSTYTVSPDHLVEIGKLAKQMGASISIHIS
                     ENLGENDDVAKRFGKSPTEILQETGILDSHTLLGHGVHLSEEDIKLLERAGAAVAHCP
                     GSNLKLGSGIADIKKYQSSDLCVALGTDGCSSSNDLDMWAVMRVAANLYAQSNGPENL
                     RASEIFRMATIEAAKALGLESSIGSIEVGKSADLISIDLNKAHLIPLHDVFAQLVFAA
                     GRGDVSDVWVDGKQVIADSKSTLIDFSELSKEVDVVVKTLHKEFNEQR"
     gene            complement(58531..59271)
                     /locus_tag="PLW53_00315"
     CDS             complement(58531..59271)
                     /locus_tag="PLW53_00315"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HRD35902.1"
                     /translation="MPKIIGLIAGTGFYDLPALKDGESRELDTLYGKAQIRSGEWNGA
                     RLIFLTRHGVGHSIPPHLINYRANIKAMKDLGVEQIIAINVVGGINPKLSPGDLCLVD
                     DFIDFTSGRQHTFSDGSKPGVQHVDLTRPYDAKIQSALRQSAKESGILLHENGVYAGF
                     NGPRFETPSEIRLAALAGATVVGMTGCPEVSLARELGIAYASIALVVNPAAGLSEAEI
                     TMDEINKALDLGKSKALTVIEGALKVLA"
     gene            complement(59276..60784)
                     /locus_tag="PLW53_00320"
     CDS             complement(59276..60784)
                     /locus_tag="PLW53_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD35903.1"
                     /translation="MKTLLEIQGVSKSFGSVVANENISLSVKEGEILALLGENGAGKS
                     TLVKAVFGLVKPDSGTIKIDGHIMDSGDTAAAIASGVGMVHQHFQLVPVMSVTENLIL
                     GDEPKRFGVVNIKKARAEAISLSEKYGLEVDPDAIIEDLPVGMAQRVEILKALRRDVK
                     LLILDEPTAVLTPQETDELLEVLRNLAKRGVGILFITHKLREVMAVADRIAVLRGGKL
                     MGTTTPKESSESKLAQMMVGREVVLRVEKGVAKPREVLLDVRNLSVKDDRKLEAVRNL
                     SLQVHAGEILGIAGVEGNGQRELVEAICSMRPRLSGEVVIKGISIGNMNPRAAHLAGV
                     SHIPEDREKHGLVTSYSIADNLVLNRFDQAPFAKGWIRNLGQVANNGESLVEKFDIRA
                     PNASLSAGSLSGGNKQKVVVARELSQQLDVVVAAQPTRGVDVGSIEFIHNQLIAARDA
                     GAAVLLVSAELDEVLSLADRVAVISNGKIVATFTSKEADRNTIGRLMAGLAS"
     gene            complement(60781..61761)
                     /locus_tag="PLW53_00325"
     CDS             complement(60781..61761)
                     /locus_tag="PLW53_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD35904.1"
                     /translation="MKLRTSHYVILTFLVAFTYFYSRDGLVATSVLYTAIQFSIPLAL
                     AAMVGVMCERTGVVNIGIEGTMLISAFTAFFAGAILQDTVRGMFVGVFTGAMMGLLLA
                     LMAVNWKMDQIIAGVILNILAAGITSFYYRQNYSIPSTLNPIDLPVVGAWPILGPVLS
                     FHGPITYFGILFILALWFGLYKTSWGLRSRAVGEHPSSADTAGVSVTKLRYINVTIAG
                     AVGGLAGSYLALEAVGIFERGFTAGKGFTALAIMIFGRWNPLGAFAAALFFGLTQAVT
                     NQLMIDEVVEIPPQFINMLPYILTILVLAISAGKVRPPAAEGIPYEKEKS"
     gene            complement(61761..62825)
                     /locus_tag="PLW53_00330"
     CDS             complement(61761..62825)
                     /locus_tag="PLW53_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD35905.1"
                     /translation="MEAKQEKKLDLSPVLVPILSILVALIFGGILVFIQGIDPLLAYK
                     VLFTTAFGSLDGIAITLAKATPLILSGLAVAICLRAGLFNIGAQGQLISGALASAWAG
                     YTFVGLPALVHIPLALIFGAFFGAIVALVAAILKAYRGVHEVITTIMLNSIVIALADY
                     LTSTPFKEPDQPLTRTPKIQDSARMPDLLGLPLGFFLAIALSITLWWIMKNTTTGFSI
                     ETIGRNKNAGWYAGISIKKTVILSMLIGGAVAGIAGAVETLSITGRFEPAFNAGLGFD
                     GITVALLGRANPLGVIPAAILIGGMRASASTVQFEAGAAPELVDLLLAMILFFVTAPL
                     LAKFFKNRKENVAMTSGWSN"
     gene            complement(62930..63961)
                     /locus_tag="PLW53_00335"
     CDS             complement(62930..63961)
                     /locus_tag="PLW53_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD35906.1"
                     /translation="MIKRVISVVAVVALTALGFAAPSKAASESDCGTGKLFCIGLVTD
                     TGKVDDKSFNQSAWEGAKAAAKSNGGFAKYIETTDPKDYATNIGQFADKKYNVIIGVG
                     FLMAEAMTKAATDYPKIKFIGVDQFQGAALPNLTGLVFPEDKAGFLAGYLAGHLTKTG
                     KTGAVLGTEVVPPVRYFGEGYRNGVAYAAKELGKKYPATKLIYHAPDNAFNDPAWGGT
                     TAQQLLSQGYDVIFGAGGNTGNGALLRVAKKKGAYCIGVDSDQWGTLPEARPCLVTSA
                     MKLIDKGVAAVIKTIVEGTDKGGNYFGAVGLAPLHDFAKKVPAAYQAKLKAITPKVLN
                     GTVKTGVKL"
     gene            64219..65229
                     /gene="mtnA"
                     /locus_tag="PLW53_00340"
     CDS             64219..65229
                     /gene="mtnA"
                     /locus_tag="PLW53_00340"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRD35907.1"
                     /translation="MRTIDFLEQGDGAITLIDQRLLPHERKILTIKTHEELIEAIQTL
                     AVRGAPALGVAGALGVALIALRSQQESWSQEQRDSAIAALRAARPTAVNLAWGVDQVT
                     PELVNGPSAVLKSALAISRADGLANRAMGKLGAAYLLEKCGTRKLRILTHCNTGSLAT
                     SDWGTALGVIRELSEQGYIEEVFADETRPLLQGSRLTAWELSEAGINYRILPDGAAAS
                     ALFSGLIDAVLIGADRIARNGDSANKIGSLGVAVAAKERGIPFLVVAPESTIDRSLAS
                     GAEIEIEFRADSEVTHFAGKAVAPVGAKSYNPAFDVTLARYITAIVTEKCVYNLLAGD
                     KL"
     gene            65276..65926
                     /locus_tag="PLW53_00345"
     CDS             65276..65926
                     /locus_tag="PLW53_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD35908.1"
                     /translation="MVSEINSLNSESEIASLIKIGKAVVDRGLVVGSGGNLSMLLKGG
                     KEFLITKTGSELDQLDQSSFALMDLDGNIAKGSAKPSSEYRVHLESYRTRKDINTCIH
                     LHPQASIFAAAIGEDIQLVTTDHLYYLRKIIRIPWIAPGTQEVAEATATALKDCNVVI
                     LENHGCVVVADNPRLAYSRALNLEEAAEMTIRSALLKVKPKLVPQEFEQYLKQQGL"
     gene            65942..66337
                     /locus_tag="PLW53_00350"
     CDS             65942..66337
                     /locus_tag="PLW53_00350"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRD35909.1"
                     /translation="MKIDWELLRREAISASKKAYAPYSNFPVGAAGLVSDGRIVSGCN
                     IENASYGLTLCAECSMISNLLMSGGGKLIAVVCVDGKGELLSPCGRCRQLLFEHGGNE
                     LQLLTPSGSQNMSVILPWGFGPDDLEKKN"
     gene            66341..67627
                     /locus_tag="PLW53_00355"
     CDS             66341..67627
                     /locus_tag="PLW53_00355"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRD35910.1"
                     /translation="MSEAFDAVEIISAKRDRNELSDLQIDWIIDAYTRGVVADEQVSA
                     LLMAILLNGMNMREISRWTDAMINSGERMNWSSLGIPTVDKHSTGGVGDKITLPLAPL
                     VAACGAAVPQLSGRGLGHTGGTLDKMESIPGWQASLSNDQMLKVLQDVGAVICAAGAG
                     LAPADKKLYALRDVTGTVPAIPLIASSIMSKKIAEGTSALVLDVKTGSGAFMSDPEKA
                     KELASVMVELGKRAGVNTRALVTAMDVPLGLTAGNALEVRESIEVLSGGGPADVVELT
                     LILAREMLDAAKVRGKDPEIALKDGSAMDRWRKMVKAQGGDPDAPLPTAKEKLVITAE
                     KSGTISSMDALKVGISAWRLGAGRARQGEAVQAGAGIEIHAKPGEKITSGSALFTLHS
                     DDSERFERAKQALEGAVSISEKGEVAERLPLVITKF"
     gene            complement(67624..68463)
                     /locus_tag="PLW53_00360"
     CDS             complement(67624..68463)
                     /locus_tag="PLW53_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD35911.1"
                     /translation="MSLDIKKTYKLFIAGAFPRSESGRSYEIRDKKGNFIANPAQASR
                     KDLRDAVLAAKNAHSSWASATAYNRGQILYRIAEIMEGRSEQFVAEIMAMESVSSKQA
                     QLQVRQAIDTWVWYAGWSDKISTIAGSTNPVAGSFFNFTVAEPLGVVGIVADEKSSLL
                     GVVLGLAPVLAAGNCAVLLASQKRPLSAITLAECLATSDVPAGVVNILTGLSDELTPQ
                     LASHMEIDGLDLSGVDSKSAAALRISSVDNLKRVHSFSAEKSPERILSFMEYKTLWHP
                     IGI"
     gene            complement(68463..69905)
                     /locus_tag="PLW53_00365"
     CDS             complement(68463..69905)
                     /locus_tag="PLW53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD35912.1"
                     /translation="MSDFEYAPAPESKAIANIKKDYGLYINGKFVASHNKKRFETINP
                     ANEEVLAKISLADEVDIDKAVKAARSAYVKVWSKLSGKERAKYLFRIARLLQERSREF
                     AVVETLDNGKPIRETRDIDIPLSAAHFFYHAGWADKLEFAGFGDKPKAHGVVAQIIPW
                     NFPLLMLAWKIAPALATGNTVVLKPAETTPLTALLFAEVCQQADLPPGVVNIITGDGS
                     TGAALVKHPDIDKVAFTGSTEVGKLIARSLAVSKTPVTLELGGKGANIVFEDAPIDQA
                     VEGIINGIFFNQGHVCCAGSRLLLQENIHDEFLNKLQKRMSKIRLGDPLDKNTDIGAI
                     NSAAQLAKIHELSSSGQAEGADIWSAPCQIPNKGFWFAPTIFTGVTQAHRIAREEIFG
                     PVLSVLTFRTPQEAIEKANNTPFGLSAGVWTDKGSRILWAGNHLKAGVIWANTFNKFD
                     PASPFGGYKESGWGREGGRHGLGAYLKGAK"
     gene            complement(69908..70855)
                     /gene="deoC"
                     /locus_tag="PLW53_00370"
     CDS             complement(69908..70855)
                     /gene="deoC"
                     /locus_tag="PLW53_00370"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRD35913.1"
                     /translation="MSALNLIDGSETSLRRFLQTLPGVDQIGAQERAAMLGTRSIKTT
                     SKKWAIDMAISMIDLTTLEGADTAGKIRSLCSKALRPDPQDASVPHVGAVCVYNDMVG
                     IARKHLDSIGGQSIPVAAVATAFPSGRASRKVKLQDTKDAISLGATEIDMVIDRGAFL
                     SGQLGEVFDEIRAIKEICSDKAHLKVILETGELVTLDNVRKASYLAMLAGADFIKTST
                     GKVTPAATAPVVLVMLEAVRDFFELSQIRIGVKPAGGIRTTKDAIKQLVLVKEIAGAE
                     WLNPELFRIGASALLNDLLMQRMKLSTGRYSSANYVTVD"
     gene            complement(70874..72499)
                     /locus_tag="PLW53_00375"
     CDS             complement(70874..72499)
                     /locus_tag="PLW53_00375"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRD35914.1"
                     /translation="MDQSLIEQVQQWISQDPDPKTRAELERLLADRNEVELRSCFSGF
                     LEFGTAGLRGKLGPGPSRMNRAVVTKTAAGLVTYMRKHNLTSVVIGRDARYGSKDFAQ
                     DSAEIMQGAGMKVYLLPRALPTPVLAYAVRELKCDVGIMVTASHNPPEDNGYKVYLGG
                     IVDQIRYEGSQIISPADTQISEYISQVPDLNTLKRSSGAEVLDNTIVDSYIKATAKLA
                     TSNSDLKVVYSAMHGVGTKTLEQVFAAANFAPPILVMEQAEPDPDFPTVKFPNPEEPG
                     AIDLSIKKAMQVSADLVIANDPDADRCAVAIADRRGNWRMLRGDEVGAILGEYLARAA
                     KDKNATLANSIVSSSILSKIAKAYQLPFAETLTGFKWIAKIDNLHFGYEEALGYAVDS
                     NSVNDKDGISAALMIVQIATDLKRESRTLNDFLDEIWSKYGYHGTKQISVRVNSVEQI
                     TTILGRFRNNTPHSIAGFRVIGFDDLEKPLSGLPPTNGVRIFLEPNIRIIVRPSGTEP
                     KIKCYIEVVSDNQEKAEEIIKGLDLELRTLLAS"
     gene            complement(72512..72952)
                     /locus_tag="PLW53_00380"
     CDS             complement(72512..72952)
                     /locus_tag="PLW53_00380"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRD35915.1"
                     /translation="MTLYAAYGSNLDPRQMLLRAPYSPHRGTGWLRGWRLTFGGEEIG
                     WEGAVATLAEDAGHSVFVSIYDLTATDEAALDQWEGVTTDLYRKIRVRVETLDGSPLC
                     YIYVLNSFEGGMPSARYLEIMVNAAREAGAPEDYIKDLSNRSTR"
     gene            complement(72949..73545)
                     /locus_tag="PLW53_00385"
     CDS             complement(72949..73545)
                     /locus_tag="PLW53_00385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRD35916.1"
                     /translation="MAPKIILASASPARYKLLQSAGITPEVIVSGVDEESSDYQNLSP
                     KELVIALAIVKAHTVGAKINYPALIIGCDSTFEFQGQSLGKPGSAENAVARAKQLRGK
                     SGLLHTGHCIIDTEKEIEISDVVSTKVFFADMSDAEIDGYVATGEPLNVAGGFTLDGR
                     SSPFISRIEGETSNVIGLSLPLLRSAINKLGYQWFDLS"
     gene            complement(73549..74853)
                     /locus_tag="PLW53_00390"
     CDS             complement(73549..74853)
                     /locus_tag="PLW53_00390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRD35917.1"
                     /translation="MLKLSVIGTGYLGATHAAAMSSLGFEVVGVDIDQKKIELLNQGK
                     VPFYEPGLEELLLAEVNSQRLKFTSDFSQIRDCDVHFICVGTPQKKDGLAADLTYVDA
                     SIASVAPHAKAGSLIVGKSTVPVGTAGRLAEMLRKVNEKVELAWNPEFLREGFAVEDT
                     LRPNRLVVGVTSDRAEEILKEVYASLLKDGVPWIRADLPTAELVKVAANSFLATKISF
                     INAMAEICEASGGDVTVLAKAIGYDPRIGSRFLQAGIGFGGGCLPKDIRAFMARSEEL
                     GAKQSLEFLREIDSINLRARARVIDLVRVDLGQDLKGKKIAVLGAAFKPDSDDVRDSP
                     ALDIAVQLSQAGADVTVHDPKAIEPARARFSHLTFSESIEGCVAGADVILHLTEWKIY
                     REINPSAIKNQVNQAIIIDGRNALDRDLWVKAGWKFRALGRA"
     gene            complement(74866..75951)
                     /locus_tag="PLW53_00395"
     CDS             complement(74866..75951)
                     /locus_tag="PLW53_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRD35918.1"
                     /translation="MKSPRAIRFFTIASIAIVGISAISWAGLGRITAAIPRVDAFAGL
                     ENRPKKESSAVNYLIVGSDTREGLSREEIKRLKVGGTDVAAGKRSDTMLLIHISKKRD
                     KAAIISIPRDSYALIPEHNNSQGKVIPAAYSKINSAYNWGGAPLLIETLESMSDLRID
                     HYVELNFVGFVRMVDALGGVEICTKKDINDPKSHLTLPAGTHVLDGVDSLKFVRTRVF
                     DGLGDLGRMKRQQEFAGAMLRKATSAGVLLNPVKMVDFINSALDSVVTDEGLSQGDLL
                     TLGKQLRNLSASNVRTLTIPLKYYNYNKNGVSAAVLWDPVLAPELFERIKNDEALIEE
                     VRPDPSSSPTTINKFKSGTAADNPCSG"
     gene            75989..76993
                     /locus_tag="PLW53_00400"
     CDS             75989..76993
                     /locus_tag="PLW53_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD35919.1"
                     /translation="MTSPLEQGNTAHLANYPPVSIILTVINEALHLRAAIKAALSSNY
                     QGELEVILAVGPSTDETWQIAKELAEQDSRITVLENISGRTPSGLNAALKLAKNQIVV
                     RIDGHSEIDKEYIRKAVETLKETGAVNVGGIMFAQGTTSFEKAVAVAMRSQIGVGSSR
                     FHVGGEAGPTDTVYLGVFQKSALDAVGGYDERFTRAQDWELNYRLRKNGGVVYFDPEL
                     KVIYRPRSSLKKLALQYFEYGRWRRAVVRQHSRTINFRYLAPPLNLILQVVSLLLALF
                     ASSLFLIPIISYLGAITLASLIIGKGLAQRIYLPIVLIFMHFCWGFGFITSPRKLITN
                     "
     gene            77023..78348
                     /locus_tag="PLW53_00405"
     CDS             77023..78348
                     /locus_tag="PLW53_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRD35920.1"
                     /translation="MKKRAFLLTSLSLIFSLGLNVISPVHARTLIPTDFRFFGSGYGH
                     GVGMSQIGARGQALESKTAVEILNYYYPGTAVTAYPDNQLIRVNIANLVSSVTLNAVG
                     SIGEIRLYQGDIPLSESPEPFGIYPGDTTALFTNFAGSVVPALSSPTAKYAAINPAPA
                     WTVRWDSATTTALLTNGATATQYKYGQIVFKSVTNLLSSYLAVTNTLRLHDEYLYGLG
                     EVPSSWPAAALEAQAIAARTYALGKLSRLRVECDCNIYNTTVDQNFVGYAKETEAIYG
                     IKWREAVDRTFVDENNALVVTLEGKPIQAFYFSSSGGVTQNVVDVWGSPLPYLTGVPD
                     PWSLDPTINRRYALWSRFVPQSVMAQAFLLPNVVSFTINSRTQTGSISSITAISSTGA
                     TATLNGELFRARVKLPSTWIHNTKAIVKLPFIAKECLPDIVERSNYCLI"
     gene            complement(78345..79268)
                     /gene="dapD"
                     /locus_tag="PLW53_00410"
     CDS             complement(78345..79268)
                     /gene="dapD"
                     /locus_tag="PLW53_00410"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:TIGR03535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRD35921.1"
                     /translation="MSKASAAYGYGISTIAKDGSILDTYFHQLGLGAAEESRVDLTSL
                     EGADNEREITRALVKTEIELDKPPASVADAYLRLHLLSHRLVKPHGLSLEGIFSILNN
                     VVWTSHGPCAIAGFDQVRLKLRAKYGYLAIYGVDKFPRMLDYVVPEGVRIADADRVRL
                     GAYLSPGTTVMHEGFVNFNAGTLGASMVEGRISAGVVVGDGSDIGGGASIMGTLSGGG
                     KEVISIGKRTLLGANSGLGISLGDDCVLEAGVYLTAGSKVLLPDGKVVKARELSGANS
                     LLIRRNSQSGALEALNKSGTWGGLNSVLHSN"
     gene            79344..79877
                     /locus_tag="PLW53_00415"
     CDS             79344..79877
                     /locus_tag="PLW53_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRD35922.1"
                     /translation="MKVAVYCASSTLVSKENLELGFELGASLAKLGHELVWGGGAISV
                     MGEVAKGARENGGITIGVIPERLMSVEFIDHDASQMHVVKDMRERKGFIENLADGFIA
                     LPGGIGTLEELLEIWVGRYLGFHNKPIAILDPSGVYGSLRLALDDLTAMNFMKPGQHE
                     LVSWCNTIDSAIKHVTS"
     gene            79874..80326
                     /locus_tag="PLW53_00420"
     CDS             79874..80326
                     /locus_tag="PLW53_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD35923.1"
                     /translation="MSMNSKSKLADFRASVTISAPIGEVWKKLVDWKSQGQWMALTKV
                     NSSADKDGSSGIGTTISAFTGIGKIGIWDEMKITAWSPETFCAVDHYGRWIKGIGEFR
                     LIEIAPGETRFDWYERIDAPAAILALIKPGILLAVNYSLAKFARGFNN"
     gene            80651..80830
                     /locus_tag="PLW53_00425"
     CDS             80651..80830
                     /locus_tag="PLW53_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HRD35924.1"
                     /translation="MAAMKPRTGDGPMEVTKEARSLVMRIPLEGGGRLVVELNQEEAR
                     NLADVLNASISLLKK"
     gene            80827..82338
                     /locus_tag="PLW53_00430"
     CDS             80827..82338
                     /locus_tag="PLW53_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRD35925.1"
                     /translation="MNLPIPKLGTVKLPGEISEKFFKDFSHIALPIKAKESKEEPEIE
                     IILSKKIIQSIEKFYSISLKDEIKKNGGTVTGKAGEVIDIPVLQASGDVVRILLVGVG
                     DSTNSHIRRASAAIGRKIKASNSSVLSFLVEKESDAQLHFISSALATYIWTQKSGTKS
                     LTPEITLVGDFAEVLERAKVLVKSTWKARDLIQTPSNIKNPAWMAAQAKSLAKSPTLS
                     LKIKSGRQLAEFGGLSAIGNSSVKNPPRFVELSYKPKGSKRAPHVVLVGKGITFDTGG
                     VSLKRPYDVMTAMKSDMAGAACVLMATIAAAELKLNVRITALLMLAENSLSGTSTRPS
                     DVITHYGGTTVEVINTDAEGRLVLADGLAYADMNLDPDYLIDVATLTGAATLGLSRYY
                     GAMYTRDNKLARKLADIGDYTGDQIWHMPLVDDYSIALESNIADFNHTADKFKFQGGS
                     VTAALFLENFVGKRKWVHLDIAGPARSEVDAGENVKGGTGYGVRLLTQWLASL"
     gene            82338..83006
                     /locus_tag="PLW53_00435"
     CDS             82338..83006
                     /locus_tag="PLW53_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD35926.1"
                     /translation="MSGIGEKGFTEKLFPERGDMRAFSNTFIPETEIIQKARARGQEI
                     GTYDATPAVGSLLRYLTHLIGASSIVEIGTGAGVSGLWIFQALSSKGLLTSIDDEVEN
                     AKLAKQAFDEAGINSSRYRLITGNSREIVGKLADSLYDIFIMRKSGDLLDGIENAHRS
                     LRPGGVLVIDRALLSGKVADPTQRDEDSVAYREVIKVIKDSATLWLPMLLPVGDGVMV
                     ATKI"
     gene            83027..83365
                     /locus_tag="PLW53_00440"
     CDS             83027..83365
                     /locus_tag="PLW53_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35927.1"
                     /translation="MFGIGMGEFLGLVILGLFLVGPDKLPEAAKDFARFIHKVRNFTT
                     YASRELKENLGPGFEDLQVTDLTPKKLAQKVLGNALDDVKPLANDLKSQAKASKKVID
                     EAAKIDPDLL"
     gene            83362..84498
                     /locus_tag="PLW53_00445"
     CDS             83362..84498
                     /locus_tag="PLW53_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRD35928.1"
                     /translation="MSFSKDEVVSQIHSALATVSDPELHRPLPDLGMVESVDFDSGLA
                     KIKILLTISGCPMRDKLKSDVSSAVTKVAGVEKVELEFGVMNETQRDNVKKLLRGGRE
                     KFIPFAQPDSLTRVWAISSGKGGVGKSSVTVNLAAALSKRGFKVGVLDADVYGHSIPR
                     LLGIEGQRPTAIDQTFIPVETNGIKVVSIEMFKPDRADPVAYRGPLLHRVLEQLLSDA
                     YWGDLDFLLLDLPPGTGDIAISLGQLIPASEIIVVTTPQIAAAEVAERAGRIAHQLKQ
                     HVIGVIENMSAYTDPVSGAQVALFGQGGGQETAKRLSQLVGVDVPLLAQISFDIKLRE
                     GGDEGSPIVFRDPESDAAKGFFAIIDQLTKRKKSLLGVPLGVAT"
     gene            complement(84466..84933)
                     /locus_tag="PLW53_00450"
     CDS             complement(84466..84933)
                     /locus_tag="PLW53_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRD35929.1"
                     /translation="MTMAGKSKLETPRESRRSLRPNYDPEVIGRVSEAFARFIGTARF
                     LVYMTVFVATWIIWNFVAPENLRFDEYPFIFLTFMLSLQASYAAPLILLAQNRQADRD
                     RVTSQEDRERDERNLAVTEFMVREIAAMRQELLADIKEMLDEKLGSDPKGNAK"
     gene            complement(84930..86171)
                     /locus_tag="PLW53_00455"
     CDS             complement(84930..86171)
                     /locus_tag="PLW53_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRD35930.1"
                     /translation="MRTEELTRVFLAKLAGTPVFDPNADRVGKIRDAIASLRQDNLAP
                     RILGFIVEVPQRRKIFIPITRVTSINDGGVFITGSLNIRRYQPRHGEIALLSELLDHK
                     TRLIENGNVVIIEDIGMELRDNKDWYAEHVHVQEKTRSFGRRSTQTLSWSSVEIEVSS
                     HIPDLSAPTEDIALLPAQDIAAALRELEVNRQSEIARTLDDEKLADVLEEMDDADRLA
                     LVSLLEGELVADVLSEMNPDDAADVLKEVGSEKAESLLKLMEDEEAQDVRRLMAYEDF
                     SAGGMMTTDPVILAGDATVAQALAVLRQKDVSAASAAQVFVCRAPLETPTGRLIGVVH
                     MQRLLREPPALSLAIVAEKETNAISADTPLNDVVRHLANYNLLAAPVVDEHDRLLGAV
                     TVDDVLDHLLPSNWRRSEAEK"
     gene            86241..87113
                     /locus_tag="PLW53_00460"
     CDS             86241..87113
                     /locus_tag="PLW53_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD35931.1"
                     /translation="MPSGRDIPLLLLGVIGIGTSGPVIALSAMPIITLIFWRNLGGAL
                     IMAPFALRNGQWLKSENRRGLAWSMAAGFVLALHFIGFFIAMRYTTVAAGTALTALQP
                     IFAAYFVKKLGGHIPRRAWIGMVISFIGVLIITGVDFQISTRAFIGDIAAIICAALAA
                     LYVLLGSKAQQTLATSTYTTLCYLTCSLTALPIALISGVQLIGFEAKQWWLLLALILG
                     AQILGHTMFNFSLKRVSPAIVSLIVFFEVPVGALLAFWWLDQLPPLGTVPGLILLLAG
                     CAIFVMRSKDEEKG"
     gene            87124..87987
                     /locus_tag="PLW53_00465"
     CDS             87124..87987
                     /locus_tag="PLW53_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRD35932.1"
                     /translation="MLIDLHTHTNASDGTDTPSGLIDKAIAAGIGVLALTDHDTTRGW
                     QEASKALLNHPSGSDMELVLGSEISCQDEDGLSIHMLGLLFDASHQGLINELDKTREH
                     RLFRMEKIITRLNEAGIEISIDEVYAMRTGDATLGRPHLADALVAKGHVASRQEAFEI
                     FLHNKSKYYVNHYSPTPEAAIKLIKQAGGVAVIAHPFASKRGKTLKVEAFDNLIAAGL
                     DGIEVDHPDHSESEKSELVSLAIARNLVITGSSDYHGDGKLNRLAQFTTNPEQWAHLE
                     SKANQRRVVKR"
     gene            87984..88595
                     /locus_tag="PLW53_00470"
     CDS             87984..88595
                     /locus_tag="PLW53_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRD35933.1"
                     /translation="MSDISTITFGLQAFVTLLVILDPPGATPIFLSVLGDRTRRQRTL
                     LAWQAAGTSLFVITLFALFGQFIVDYLEISMPALRGAGGLLLLLVSLDLLKGSDFSGG
                     GKIPKNLALVPLGTPLLAGPGAIVTIMLFSQEVSNTSMTLALSLAVLGAHLIIGVTLM
                     FSLGIVKIIKESGVALLAKIAGLLTAAIAFEMLMTGIKDLFAS"
     gene            88592..89374
                     /locus_tag="PLW53_00475"
     CDS             88592..89374
                     /locus_tag="PLW53_00475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRD35934.1"
                     /translation="MSLGFFSPDSITWKVHSDPSMFVGGIRALLEQALHPEAMAGVAA
                     HSNFREDAWGRLERTGDYVATLTFASKEKAEKLAARVRGVHEKLKLDDQRLLLWVHMA
                     MVDSFLDTALRSGLVLSEKERDQYLEEMVVFARLVGIDEEKVPRNVAQLDKYFIDIKD
                     QLSASDDAKRAALFIALPPLPPLLRFGTPIAPLWGGITSIAAASLPKWAKSLYGWPTL
                     PGQDVATNIALRSLRSALLLVPESLRQTPEMKFAFAQVGAEK"
     gene            89371..90081
                     /locus_tag="PLW53_00480"
     CDS             89371..90081
                     /locus_tag="PLW53_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD35935.1"
                     /translation="MKVLVVANSAGGVGKSTSVHCIAVAASEYGKKVLVIDADPSAGL
                     TFACGIENPRVSTKEFLSGQFSLDASTIKSGERFSIIASSSRLLALDFDQVISQAKLT
                     ELLESFDLVIIDTPTGVSRITTYFMAVANLILLPSTTEILSLRGALNSKDYAESAGHS
                     GQLQLLITKTTQELDEEFKSLIGDDFSLLEPTIRSSAQVCLSQSQSKSILTLDTKSEV
                     AADYREVTYLLLEKLELI"
     gene            complement(90083..90346)
                     /locus_tag="PLW53_00485"
     CDS             complement(90083..90346)
                     /locus_tag="PLW53_00485"
                     /inference="COORDINATES: protein motif:HMM:NF022749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HRD35936.1"
                     /translation="MSTKKSGKSESAKSEVRISVADQLRELTIETSLDRDEVLALVNK
                     ALTSSAPLVLTDIRGRQFVVPAGKIGSIEIGDIAERRVGFAGA"
     gene            complement(90403..91020)
                     /locus_tag="PLW53_00490"
     CDS             complement(90403..91020)
                     /locus_tag="PLW53_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD35937.1"
                     /translation="MSNSEKVDNRVRLPRDERRALLLNAALEVFTVSGYHAAAMDEIA
                     DRAGVSKPVLYQHFPSKLDLYLALLDVHIDSLVFAIQKAIASNKENKNRVKATVGAYF
                     EFIDDEGGAFRLLFESDMSAEPQVRQRLDRMTYDCAVAVSAVISLDTGFPKEESMVLA
                     IGLIGCAQITARHWLERDGKINRSEATSLVTNLLWRGISGFPLQS"
     gene            91086..91949
                     /gene="mshB"
                     /locus_tag="PLW53_00495"
     CDS             91086..91949
                     /gene="mshB"
                     /locus_tag="PLW53_00495"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HRD35938.1"
                     /translation="MRKPRLLLVHAHPDDETINNGVTMAKYAAMGYGVTLVTCTRGEE
                     GEILVPELEHLASGKEDALGPIREVELANAMKVLGVVDHRFLGSPSKSWRDSGMMGSE
                     PNSRKDNFWNADVDQAAESLVEVILEIKPDVMITYDEIGGYGHPDHIQAHRVAMRASE
                     LAAQRGWQIQKIYWNTIPISIIEQGIEAMKGSGSDFWGVEKAEDFPFAQPDNLVTTVI
                     DGQDFVEKKMDAMRAHPTQIAVDGPFFALSDNLGFKVWGQEYYRLVYGKASAPFNEEG
                     RETDLFSGIQL"
     gene            91946..92257
                     /locus_tag="PLW53_00500"
     CDS             91946..92257
                     /locus_tag="PLW53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF040328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6113 family protein"
                     /protein_id="HRD35939.1"
                     /translation="MKSLISLLFGALVGVSGTFLHNAYRPFGLIVSLLALFIGLRLVR
                     NMYLSKLSLALFAFGWLFVIVRASSLGNGGEVLIEANAYGNLFVFGGAALISWRLLKR
                     I"
     gene            complement(92261..93673)
                     /gene="cysS"
                     /locus_tag="PLW53_00505"
     CDS             complement(92261..93673)
                     /gene="cysS"
                     /locus_tag="PLW53_00505"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRD35940.1"
                     /translation="MKREPKPNNGLHIYDTLERKVLPITASDGQVVRIYCCGPTVYRD
                     AHVGNLRTFLLSDLVRRTLEFSGLKVKMVQNITDVGHMSEDFEEDKMLAQSKLEKRDP
                     YSIAREYEEKFHKDLARLNILPAHNYPRASECIEMMLEHIAKLTELELAYQGDDSSIY
                     FDSTKFDSYGQLSGNRLDALKPGHRYEYVEAGAKRFHADWALWKAAGNRKEMIWQTAY
                     GPGFPGWHIECSAMSLHYLQGHVDLHIGGIDLRFPHHENERAQSNPLVKGEAVSAWLH
                     GEHLLFEGRKMSKSSGNVVLLSDVITRGFDPLSLRFCFLENRYRSQMDLSWNSISAAH
                     STLQRLRAKYQQWKLAPRDYSDEASAFVLAILAHFQADLDTPQVMIKLRSLEKDDQFS
                     DGQKAAILENLEPLLALKLLEEMPIKELPDEARVLLEARQRARENKDFALSDQMRDQL
                     LLLGIAINDSPSGQSWSWRS"
     gene            93769..94095
                     /locus_tag="PLW53_00510"
     CDS             93769..94095
                     /locus_tag="PLW53_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRD35941.1"
                     /translation="MTYIIALPCVDVMDKSCIEECPVDCIYEGDRMMYIQPDECVDCG
                     ACEPVCPVEAIYYEDDVPAQYSEYKEVNAEFFDELGSPGGAAKVGRVGKDHPVLLQIQ
                     KKPAGE"
     gene            94106..95179
                     /gene="dapC"
                     /locus_tag="PLW53_00515"
     CDS             94106..95179
                     /gene="dapC"
                     /locus_tag="PLW53_00515"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRD35942.1"
                     /translation="MKLPDFPWDALAPYGQKARAHSQGIIDLSQGTPVDPTPDFIQEE
                     LKSSSDSPSYPLTTGSAALREALKKYCVETLGASGDFDVLPTIGSKELVALLAYLLEA
                     KSVLIPEIAYPTYKVGALLARAEVLEVGIDANSWPKKADLAWINSPSNPTGRVHSREE
                     LEACLTWARKSGATLASDECYLPFTNGIKAQSILAIANGDNTSVLAVHSLSKRSNMAG
                     YRGAFVAGDPKLIAQLLEIRKHMGMMLPLPIQKSMAIALSDQSHVDKQAGRYQARREV
                     LAKALIAAGFKIEFSEAGLYIWCSRDEKDWDSVNWFAEIGILVTPGSFYGSKGDRCVR
                     IALTASDENISSAASRIIAAVNR"
     gene            95176..96153
                     /locus_tag="PLW53_00520"
     CDS             95176..96153
                     /locus_tag="PLW53_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRD35943.1"
                     /translation="MINKAILLVGGRGTRLAELTKNTPKPMLKVAGAPVTEHQIARAQ
                     AAGVTEIVLATSYLAEVFEPYFGDGSKFGISIKYAIEKQPLGTGGAISNAAKFLNLAE
                     DESVFIFNGDVLSGHDLRAQAQFHESKGADVTLHLVRVDDARAYGCVPLNQDGKVLEF
                     LEKMDQPKVNTINAGCYIFNRSAIAQIPQDSVVSVERQTFPELLKSKGALYGYVDDSY
                     WIDMGTPASYIKASRDLISNSAISPATRGVVDGVMIAKDAAVDATAIISDGSAIESGA
                     VIGANCQIKGSIISAGASIGDGSIIDNCYIAPNVSIASNEKIFEKIISN"
     gene            96349..96609
                     /locus_tag="PLW53_00525"
     CDS             96349..96609
                     /locus_tag="PLW53_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRD35944.1"
                     /translation="MPGPSIQLGNGEIVELSWQERALCAQTDPEAFFPEKGGSTREAK
                     RVCLSCEVRQECLEYALAHDERFGIWGGLSERERRRLKRRPA"
     gene            96697..99774
                     /locus_tag="PLW53_00530"
     CDS             96697..99774
                     /locus_tag="PLW53_00530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD35945.1"
                     /translation="MPTLDNNFVTAIIVSHNGALWLPEVVASLAKQKRAIDQIIAIDT
                     GSEDSSVKLLRGAGIATISTAKETGFGAAIKEALGNSRVKRGDESETEWLWLIHDDCA
                     PDAFALKELLAALDERPAVAMAGPKLRGWHDRNHLLELGISIASNGARWTGLEYREAD
                     QGQHDGVSEVLAVSTAGALIRRDVFEELDGFDPELSLFRDDVDFGWRVHTAGHSVIAV
                     PKAVAYHAEAASNERRRIDVSDAFLHRPLLLDRRHAAYVLMANTSWWLLPLIALQLLS
                     ASLFRAIGYLLAKLPGYALDEIAAVALVILQPQDLFRARAKRRKNRLVSSRVISRFVP
                     PRGSQLQLAIERAGSAISRSWRRSSIYEESGVTSSALDLNDEALENADIDLVQAPSPI
                     RWLTRRPILSVSLLVILLTLIASRNRFGAIVGGALAATPTKAFELFSMYSDSWHTVGL
                     GSSTSAPPWVAYIGFGSLLTGANSSLFITGLFLCAVPLALWGSYLLAKKFTNLHFLAL
                     IAALLYTFSPTTLSAINSGRVGTLVLVVIGPWLIRSLFGLEQLENLSWRKTFWIALLL
                     TFVCAFSPMTFMSILLWQIILVIFDVIAFNSKNNAMDKTVFDRRNARRIAVTITPLIV
                     NAPWSIEFILHPSRILLDPGLSLAGGEVLSLLLGNPGGVGAPPIWIISPILLIAVIAI
                     FVSKTARLGEVALFFIAIAALLGSRQVSGHGSFTPEQLWVGSLLVIPTLTALLAAVIM
                     IDNYLPSLSQSNIDYRHILLGFTSLISALSILASATWWLGSANSAPVQANSKSALPAF
                     LSASAQTEGRYKTLVLRAESSKVKYFIARDKDLQLGQADVITGLAPVVTKAINDLIAG
                     SGVSSSKVFAEFGIRYLFLANPINEELVRTIDGAGGFTRAASTDEGITWKVPGALGHI
                     SFLSQDGSYSVLPSGEVGAAGTLTSAGVIIITEKYDNRWKMLLNGTYLPLTQTENGVP
                     RFYVSEPGDFLIFHDGTSRRAWVSLQLLTFLTLIILALPARRRRSQMSEQELA"
     gene            99771..101078
                     /locus_tag="PLW53_00535"
     CDS             99771..101078
                     /locus_tag="PLW53_00535"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HRD35946.1"
                     /translation="MKETRASFALLIFIIIAHIAHLLPQSSSNSVVTQSYPATACPAG
                     VSGAKATALLATKNIEIRNINQPSAELKKNGSGSYPLNRGAILVAGNPANTNVIMTRS
                     GKWSSAATCTISGPINWFVGGTANVTSQSKLIMINSGLSDALIDLTAYSESGASQSLP
                     VTVKASSEKVVRIDSLSPGSERIVLKVETRSGRITSYLLDERVRGLSNIGGDFVPAMS
                     EASKELVIAGLAVKLGSSSSIKHTLRLMSVGEVDASASVEIISPDGVYVPVGIGEISL
                     NAREVTDIDLSSVDFGSKAFALKVSATEEIVASVLSEVKSGSVSDFTWSAPSQSFNTV
                     SFNIYGLEPVISFVGEKVILSIVWRDRGGKSNSELIRGDEIVNWRVPPNTRLLTISSA
                     TPVVAAMSWISGDGVARLGIAPSTNLESATKPIADIAVIQPRG"
     gene            101121..101507
                     /locus_tag="PLW53_00540"
     CDS             101121..101507
                     /locus_tag="PLW53_00540"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HRD35947.1"
                     /translation="MSSTNSSRPNSRPSAVGRRCTRSGCSAHAIKTLTYIYSDSTALI
                     GPLSTYVEPHAYDLCIEHSAKLTVPQGWSVIEHNPDREIVEPSPEDLIAIADAIRQSF
                     AEEIAPKAVTPEVGRRGHLRAIPDNK"
     gene            101526..102914
                     /locus_tag="PLW53_00545"
     CDS             101526..102914
                     /locus_tag="PLW53_00545"
                     /inference="COORDINATES: protein motif:HMM:NF007088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRD35948.1"
                     /translation="MDQELLDKVIKAYDIRGLVDEEISADFTFALGIAFAKFIERERE
                     PATIVVGEDMRPSSPDLATAFSDGITSQGLDVIRIGLCSTDQLYFASGALNMPGAMFT
                     ASHNPAQYNGIKLCRSGAKPIGQESGLAWIKSAIAQGFPIPNRPMGKERSQDLLADYV
                     KFLFSLFPDKSFAPDFQSRPLRIAIDAGNGMGGHTAPAVMREINAIVDGIYFELDGSF
                     PNHEANPIEPKNLVDLQNLVKEKGSDIGLAFDGDADRCFLVDEKGNLVSPSALTALIA
                     KRELIKNPGASIIYNLICSKAVAEVITESGGRALRSRVGHSYIKQMMADEGAVFGGEH
                     SGHFYFKDFWSADSGMLAALHAIAALLESQIPLSELMQSFNRYSLSGEINTTVVDQDK
                     AIDKIKSYFKDYEPGLTFDELDGLTVNAKNWWFNLRSSNTEPLLRLNVEADTQEQMAS
                     MRDLVLQLIRNG"
     gene            102968..104248
                     /gene="ahcY"
                     /locus_tag="PLW53_00550"
     CDS             102968..104248
                     /gene="ahcY"
                     /locus_tag="PLW53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRD35949.1"
                     /translation="MNPTTMTTPKHDIANASLAAEGKKRIEWAERNMPVLAQIRERFE
                     KEQPFKGVRFAACMHVTTETANLMRTLKAGGAELALCASNPLSTQDDTAAALVYEYGI
                     SVFARNAVDRDGYYSHINAALDIAPHQVFDDGCDLVNTLHTTRTELLPNITGGCEETT
                     TGVIRLQQMAKDGALTFPMIAVNDTDTKHMFDNRYGTGQSTIDAIFRATNQLIAGKVF
                     VVSGFGYCGKGVAERARGMGAEVVITEIDPTKALDALMQGYRVMPMAEAAKVGDIFIT
                     VTGNRTVISAEHFQLMKDGAILANAGHFDIEIDVAWLEKNSQKNPKIRHQTDEYLLGN
                     GRRILLIAEGRLVNLGAAEGHPAVVMDMSFSDQALTAEWLVKSAKGLGAGVHMVPVEI
                     DKEVARLKLKSMGATIDTLTPIQNEYLNSWKHGS"
     gene            104248..104937
                     /gene="mtrA"
                     /locus_tag="PLW53_00555"
     CDS             104248..104937
                     /gene="mtrA"
                     /locus_tag="PLW53_00555"
                     /inference="COORDINATES: protein motif:HMM:NF040689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HRD35950.1"
                     /translation="MTSVMVVDDDQDLAEMLGIVLNGVGIEVDLVSRGDEVLEVFRNN
                     PPDLVLLDIMLPGLDGIEVCKEIRKHSMRVPIVMLTAKSDTHDIVRGLEAGADDYMVK
                     PFKPSELVARIRTRLRRTGGEISSTLTIGDLAIDIVEHTVTRAGKEIPLTRLEFDLLV
                     SLAKEPGRVFTREALLGEVWGYRHSTDTRLVNVHIQRLRAKIEHDPENPTIIGTVRGI
                     GYKAGALGNRS"
     gene            104952..106490
                     /gene="mtrB"
                     /locus_tag="PLW53_00560"
     CDS             104952..106490
                     /gene="mtrB"
                     /locus_tag="PLW53_00560"
                     /inference="COORDINATES: protein motif:HMM:NF040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HRD35951.1"
                     /translation="MKITQYFRTSLFRKVFSVSSIISIALIYFLGSNLYNRISDGIIE
                     EKISAAISEGEAAIQYADYRFLIGSLNKNTDYKTIVDEIVKSTNVSAKASGREIALLS
                     STGKRVAGIPSRSTSNFLEPSSIPNELRQLVKEDDEIHWQRTNLRYFNGESVAGVAVG
                     HRIDIKSVGRYEMYVLFDFASQQQTIDLIGRSMWGTGLILMALIMVIASVVLRQVIKP
                     VRYAAEVAEQLSAGDLLKRMQVKGSDEVARLGTAFNDMADTLAKQITRLENLSRLQQR
                     FVSDVSHELRTPLTTIRMASDVIFAAREGFDPTIARSAELLLSQIDRFEELLTDLLEV
                     SRFDAQVATLSLSKIDLVQLVNRCVEDLAMDAKQRDVKIRIDVPEDEVLIDGDSRRIE
                     RIMRNLLGNAIDHAEGNPVDLTVKASSSAVSLAVRDYGVGIATHHLERVFDRFWRADP
                     SRSRIRGGTGLGLSIAREDANLHGGDIKLWSELGAGAQFVLTLPLTQGQPITEVPIGE
                     LPTH"
     gene            106538..107239
                     /locus_tag="PLW53_00565"
     CDS             106538..107239
                     /locus_tag="PLW53_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRD35952.1"
                     /translation="MDFDKRKEGIFSALTSLVFPKICIYCQVEFAPLCLECQQFWLAP
                     VQRRYVKGLAVNSVLAYNAASSRIIIQAKESRNKVAQFWMARALSTALEKFELDNPAL
                     SVSHCLLVPIPSTKTAIRKRGGSFLHPILDQLVALQKQKNGVRWRWKELLIHQKSVRD
                     QSELSYSARQSNMARAFRLRSDDLVRERPILLIDDVLTTGATLDSAFRALRERNLTVL
                     GAATVCASAHRLLIR"
     gene            107271..109952
                     /gene="secA"
                     /locus_tag="PLW53_00570"
     CDS             107271..109952
                     /gene="secA"
                     /locus_tag="PLW53_00570"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRD35953.1"
                     /translation="MVAILDKILRAGEGRALKQLEKIADLVNSHEANYVAMSDEQLRA
                     MTDTFKARLAAGEDLDDLLPEAFALVREASKRTLGQRHYDVQIMGGAALHRGNIAEMR
                     TGEGKTLVATLPSYLNALTGLGVHIVTTNDYLAERDSEWMGRIHRFLGLKVGVILASM
                     SPAERREQYNCDITYGTNNEFGFDYLRDNMAWNLADCVQREHNFAIVDEVDSILIDEA
                     RTPLIISGPADKATKWYQEFANIASRLTRGEHYEVDEKKRNTGILDAGVSKVEELLGI
                     ENLYEAVNTPMIGYLNNALKAKELFKRDKDYVIMSGELLIVDEHTGRVLSGRRYSEGL
                     HQALEAKERVEIKDENQTLATITLQNYFRMYDKLAGMTGTAMTEASEFMQIYKLGVIP
                     IPTNKVMQRLDQSDLVYKTEDAKFEAVANDIAQRHRRGQPVLVGTVSVEKSEVLSNAL
                     RRKGIPHEVLNAKQHEREAAIIARAGTVGAVTVSTNMAGRGTDIMLGGNPEFMADYEL
                     QRQGISPVENPEEYEAMWPAEIERQKAAVSKGHDEVVALGGLYVLGTERHESRRIDNQ
                     LRGRSGRQGDPGESRFYLSLQDELMRRFNSALVERFLSAAGIPEDTPIESKMVTNAIK
                     SAQTQVESQNFEMRKNVLKYDDVMNRQREVIYRERREVLEGADIKEQVTGFIEDTIRG
                     YVGAATNEGFPEEWDLDQLWTALKATYPISFKVEDIEAEVGGRTGLDTDFLTAKVLDD
                     IFAAYAKREQDLGAEVLRELERKILLSVLDRKWREHLYEMDYLQEGIGLRAMAQRDPL
                     VEYQKEGYELFSAMMDAIKEELVGFLFNVEVKVENEHVEAKGVTPAAPKQELRYSSPS
                     ESGQASNSSPAVNGGVSRNSPCPCGSGKKYKRCHGAA"
     gene            complement(109957..110481)
                     /locus_tag="PLW53_00575"
     CDS             complement(109957..110481)
                     /locus_tag="PLW53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF039460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HRD35954.1"
                     /translation="MKNEAEIGSKGFDLSDYDLLDHPALELFEQNSFYSTPLFGRDVS
                     QESNKLYLIPTSHGENYEADFAPTPSSLEELPNATRWTLTYLITALEIMAARRPASQV
                     ARTTHRYTYNSLLTQVGSLKEVPKIKSVRRSQPIVGVVELNATLIFKERIRALVARFE
                     GVDHKWLCTELSLI"
     gene            110617..111255
                     /locus_tag="PLW53_00580"
     CDS             110617..111255
                     /locus_tag="PLW53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HRD35955.1"
                     /translation="MKNTNKLQKNSFNFSIRSYLKTILGGCLIAVSFISALVISNSSS
                     RMITVWSAAVDLDKGEVITAEDVVISKVLLPGNAGNYIDGQVSIIGSSVVRAIGIDEL
                     IPAYALSSQVDEQLQQVPIAIAFSQVPKDIGSGQRVDIYGITKSELRTQSLESGRYKS
                     RLIASDLVIDQVDNQANTLGGDLVITLLVPRTIIPNLIEDMGNYDFILVGNR"
     gene            111252..112253
                     /locus_tag="PLW53_00585"
     CDS             111252..112253
                     /locus_tag="PLW53_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35956.1"
                     /translation="MKINLITALSSHQVEDQVIEVLLKHDFQLQKRLLSPSDFDLKLI
                     SSISALRILIISDKDFGLNWREIRRESDENLSILILDVDKRFSSDEILQQANQALRGS
                     NEALPTGDSIRRDSWVLFTGSDGSPGISTLALNTAQEYSKLAQMLLIDADLSQQSLSQ
                     MVGERVSYQRSALNNALSLQSIASFDEIESREDESVFIDVGSAPVMSQAVSDRRTEGK
                     FFMQALNSCSHLIYIIHQDSRALYQLEQFEEFLKMFSSKLNVIYLLNKESSSSSRPLF
                     RKSFRSKIDGKPHFFMPYEYGNLERARNRYATLSEVNSRSSLSRALRELAIYLHKII"
     gene            112269..113747
                     /locus_tag="PLW53_00590"
     CDS             112269..113747
                     /locus_tag="PLW53_00590"
                     /inference="COORDINATES: protein motif:HMM:NF023702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD35957.1"
                     /translation="MPFRHLLSDQSSLTSDDVLRLGELTAEWQLLADISFADLVLWIP
                     KRKDEKSWPEGHRTIAQIRPMTAATVFAQDLIGNEVAWGSRPDIDQALSSGEIVRDSR
                     AEQVGEIKIKVECIPVFFAGRVIAVISRHRNLETMRTPSRLELNYREIANHIYRMIAE
                     GNFPVKDNVYLAEAAPRVGDGLVRLNVAGEVLFASPNARSAFNRIGWDKDLEGQNLGR
                     VLDSLISQSSPLNPREENWQITMSGKQLRRDEFENSGGVLDLLAMPLTEGDNRIGAVV
                     LVHNVTELRRKDRALISKDATIREIHHRVKNNLQTVSALLRLQARRIDDPKASAAIEE
                     AVRRVVSIALVHETLSATAQDLVQFDEVITKIIQGASELNIRPNEIQIKKVGEFGLIS
                     SMVATPLALVLTELIHNALEHGLSQSGDLVELRIDRSGKSMTVTVVDNGAGIPRDFSL
                     ESNTNLGLQIVQTLTKNELAGKIEFIKPDIGTQVQISFPLER"
     gene            complement(113752..114021)
                     /locus_tag="PLW53_00595"
     CDS             complement(113752..114021)
                     /locus_tag="PLW53_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRD35958.1"
                     /translation="MDWRHRAACRDIDPELFFPVGNTGPAIAQIEEAKKVCMPCIVRQ
                     DCLQWALESGQDSGVWGGLSEDERRAMKRRAARNRARLSENNFEE"
     gene            complement(114255..114326)
                     /locus_tag="PLW53_00600"
     tRNA            complement(114255..114326)
                     /locus_tag="PLW53_00600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114291..114293),aa:Arg,seq:ccg)
     gene            114371..114892
                     /gene="msrA"
                     /locus_tag="PLW53_00605"
     CDS             114371..114892
                     /gene="msrA"
                     /locus_tag="PLW53_00605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRD35959.1"
                     /translation="MKERAYFATGCFWGAERRFWNLSGVISTSVGYMGGNTKSPTYQE
                     VCTGKTNHAEMVEVVFDPEQISYQRLLEEFWVMHDPTSLNKQGGDIGTQYRSAIFTTT
                     SEQLELARKTRQVYNDALKSAGLGEIVTQIQSADGHEYFLAEEYHQRYLEKNPNGYDC
                     HSSTGIAYPALKA"
     gene            114903..115361
                     /gene="msrB"
                     /locus_tag="PLW53_00610"
     CDS             114903..115361
                     /gene="msrB"
                     /locus_tag="PLW53_00610"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRD35960.1"
                     /translation="MSYSESELPKINPKTGKAFPVQLDDESLAKSLDPLSYDVLRKAG
                     TEVAFTGKYYESETIGVYKCKACSAELFRSETKFHSGCGWPSFYAPKSDDAVVLLEDR
                     SLAPRIRTEVRCASCGSHLGHVFEGEGYAVPTDQRWCINSVALTLEPKNS"
     gene            complement(115358..115987)
                     /locus_tag="PLW53_00615"
     CDS             complement(115358..115987)
                     /locus_tag="PLW53_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35961.1"
                     /translation="MAITLAQSRKIAAEIAKKNPRFKPIIKQSGPCPLGLNSKKPLES
                     NFSTLASSILSQQLSTKAAATIIGRVKNLSGGRISPSGISRISRSDLRQAGCSTAKAR
                     ALEELAQAALSSKVSMSSLHRLDDEEIREQLLPLFGIGVWTVEMFLMFQLGRQDVWPV
                     GDLGVRRGWEKVHRMRGEIKPAELLKLGEIYRPYRSHLAWYCWRATEIL"
     gene            116094..117101
                     /locus_tag="PLW53_00620"
     CDS             116094..117101
                     /locus_tag="PLW53_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRD35962.1"
                     /translation="MAAGIKEVATEAGVSIATVSRALRGLNHVNPETRKKILDAAEKL
                     GYPIPQSSAKALFGRTNSVGVVAPFISRWYFAQVINGAEQALREAGLDLLLYNFSQMK
                     GRERLFQHQLLKGRVDALIVISLPPTEEEFDSMLSLGIPIALVGMHHAQCSSVAIDDV
                     AGARTATQHLVNLGHKKIALLSGRPDDPFGFSVPQDRRKGFMQVLAESGLEFKPTREV
                     FGDFTMHGASRAMDDLLARADRPTAIFCQSDEMALGALQAIRRNGLKVPDDISIIGFD
                     GHEMAEFSDLTTIEQPVSLMGEMAAWSIMERLKLPKSESHSLTLPTTLVVRNSTRRLE
                     A"
     gene            117122..118762
                     /locus_tag="PLW53_00625"
     CDS             117122..118762
                     /locus_tag="PLW53_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRD35963.1"
                     /translation="MDWWSNSAIYQVYPRSFQDTNGDGEGDLVGVISRLSYLKSLGID
                     AIWLSPFYLSPNNDGGYDVEDPRAVDPRFGSMEDAVNLIKAAHKSDLRIIVDLVPNHF
                     STEHQWFKAALQSLPGSLERSRFHFYDGRGESGEIPPNNWCSIFGGPAWSRVKEVDGK
                     PGQWYLHLFDSTQADLNWENDEVKKDFEKTLRFWLDKGVDGFRIDVAHGLVKEDIFKD
                     HRDPEGLTRALRLDVSDMDRVERESLLADVPFFDKEGVHEIYRSWRKILDSYEGQKMS
                     VAEAWVHPSSRAMRYVRSDELHQIFNFDFLIAPWDSEFLVSAINKTLSEVGQVGASPT
                     WVLSNHDSPRLVTRLGGGQLGRQRARAMALLTHGLPGGIYIYQGEELGLEDGQLEDKD
                     RQDPVFFRTKGVDKGRDGARIPIPWSSQLPNYGFTTTKPWLPIPSTWQDQCVEAQVDS
                     DSHLTLYRKSLHMRKLLSRQIAQSSAITWIDTPKGVLAFSRGNDFFLYANTTGQSVEL
                     KLESNSQIILSSSAKAQLKSDTLTLPADSTVWLGKITK"
     gene            118890..120242
                     /locus_tag="PLW53_00630"
     CDS             118890..120242
                     /locus_tag="PLW53_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD35964.1"
                     /translation="MTASLRRRRLLLATSLFAALSLVLSACSSSDDSAGGANCDAYEA
                     YQGYDGTEVEIYSSIRSPEAEIYQESFAEFEECTGITINWNGTAEFEAQLPVRVEGGT
                     APDLAVFPQPGLLKAMVATGKMFPATSAVSANADEFYGPDWKAYGTVDGTFYAAPLGA
                     NVKSFVWYSPKTFADNGWTIPTTWPELLALSDKIAAEGKVQPWCVGFGSGDATGWVGT
                     DWMEDIMLRVNDAATYDAWVNHTIPFNDPKVAAVLKEAGKVLKNAKYVGNVSAIATTT
                     FQEGGAGIVDGSCAMHRQASFIGGILSADYGATIVTPEDTDVEGGITTFYFPGVTADQ
                     RPALGGGEFVGAFSDRGEVGAVQLYLTSPEWNNKKAALGGWFSANKGLDTANVADPVN
                     KNAVEILKNATIFRFDGSDAMPAAVGAGSFWKEMTAWVAENKSDKAVLDAIEKSWPKS
                     "
     gene            120419..121420
                     /locus_tag="PLW53_00635"
     CDS             120419..121420
                     /locus_tag="PLW53_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD35965.1"
                     /translation="MLELAARQSQLEQAASKLTGLVITVIVFAIVVSLAFYLAERTSE
                     RVQRYVKYFLFLAPALIFLFIGLIAPAIRTLYLSFRDARGDSFVGLENYIWAFTQPEI
                     LQILRNTGIWMVVAPIASTALGLTIAVLTDRMKRPGLVKSLIFMPMAISFVGAGIIWK
                     FVYQFQPNERVAEIGLLSQIAEWLGFVPTNWILTAPLNTFLLIVVFVWIQTGFAMVIL
                     SAAIKAIPEEILEASMLDGASGWKRFGKVTVPMIRGTIIVVLSTITIGALKVFDIIRT
                     MTGGNFKTSVIANEMYNQSFRALNYGTGSALAIILFLGVIPLVAYNVMHLRRERSER"
     gene            121420..122343
                     /locus_tag="PLW53_00640"
     CDS             121420..122343
                     /locus_tag="PLW53_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRD35966.1"
                     /translation="MAKAIKQGKVEVVKSHLSSPWASFVAILVAVLWTIPTFGLFVTS
                     LRPQTGINSSGWWTVLTKPEFTLENYSRVLFEGRGATPPLSQYFVNTFAIVIPSTIIP
                     ITIAIFAAYAIAWFDFKGRNFIFFGIFALQVIPLQMSLIPLLQLFSGGLHIGSVTVIP
                     SFGITGTFIPIWLAHTMFGLPLAIFLLHNFIAQLPRELMEAARVDGADYFKLFRQIVL
                     PLSIPAIASFAIFQFLWVWNDLLVALTFGGGKREIAPLMVRLAEMTGTRGGDWELLTA
                     GAFVSILVPVAVFFALQRYFVRGLLAGSVKG"
     gene            122385..123533
                     /locus_tag="PLW53_00645"
     CDS             122385..123533
                     /locus_tag="PLW53_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD35967.1"
                     /translation="MAEVVRAPGLNELRGRKSAKWRFFPSDVLPLPVAEMDFPIAEPI
                     KQALHDMVNRSDTGYLGPMPELFEAFSKFADTRWGWKVDPAHIRTATDAGVAAVEVIR
                     TLLKPGEKLMVNSPVYENFWTWPKEVSATLVDVPLKQEGENYWLDLDGIEREYKAGVK
                     VHLLCHPHNPVGVIFEKEVLVTLASLAKKYGVVIISDEIHAPLVYKGRNFQPFMAISD
                     DAREVCVAISGSGKAFNLAGLKCALIITGSDELKQRINAMPESVGYRASIFGAVAATA
                     GFSQSIDWLDGVIATLEENKVLIRNLIDSKIPSITYREPDFSYLAWLDLSALNLGDDP
                     AKHLLERGKVAFKGGYMYGPNHKQYIRFNFGTSPEIITEAFDRILKSL"
     gene            123530..125071
                     /locus_tag="PLW53_00650"
     CDS             123530..125071
                     /locus_tag="PLW53_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD35968.1"
                     /translation="MKRYDAIVIGSGHNGLVAACYLAKAGKSVLVLEKSASLGGATTS
                     VKAFKGVDAKLSRYSYLVSLLPDQIVKDLSLDFETIGRKVSSYTPYFDGESDKGLLIN
                     SEFDQESRDSLSELTEGDDEAIAWESFYQSIKEFAEVVAPTMLKPVPTETEIKELVDP
                     LTWVELVENTLAQSLNDNFFDDLVKGVVLTDGLIGTFTSADDHLANKCFIYHVIGNSS
                     GQWRVPKGGMGVLVDQLLIRAKQLGVEIATSQEVTSITQGDKHLLINTVDGQSYEARV
                     ALANCAPKVLEKVADFKAPPLRDGSQVKINMVLKELPKLKSGVDPKVAFAGTFHVNES
                     YFELEQAFAQASLGQIPDVIPSELYCHTLTDPTILTPDLIAAGYQSLTLFALHLPASL
                     FDNDHDRIKAEVSKRILDGFSQYLERPIESYLAKDSDGNLCIEVKTPQELEKELGLPR
                     GNIFHSDLEFPWKQDGDSRKWGVETNSERIFIAGAGALRGGGVSGIAGHNAAMAALET
                     LARLP"
     gene            125081..125230
                     /locus_tag="PLW53_00655"
     CDS             125081..125230
                     /locus_tag="PLW53_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35969.1"
                     /translation="MFKAIRRITALVALIAVAIKGALSFLSWVEKQEDVEAVWSEDED
                     LEEAF"
     gene            125230..125610
                     /locus_tag="PLW53_00660"
     CDS             125230..125610
                     /locus_tag="PLW53_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35970.1"
                     /translation="MSDTYIPGTCNLGKAEVRSRQIVALVGLVASLVLATGLIVSSAP
                     QASGLTLFAPLMVFAVGFIQSRRKFCLAYGLAGTFNLGKLGQISKVANPEDKAADRKT
                     ALSILAQATALALGLTGAITLLLL"
     gene            125731..126075
                     /locus_tag="PLW53_00665"
     CDS             125731..126075
                     /locus_tag="PLW53_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRD35971.1"
                     /translation="MKIIIHNHGDSLAIDFEKIAVERLERLTRFSIPIERIDVDVKHE
                     ANPRFGKSSHRVILTSRGSGPLLRAEGSGFNDLAAFDEAAEAVELQLRKRHERSKDID
                     RTSLRKLRGAKN"
     gene            126152..127333
                     /locus_tag="PLW53_00670"
     CDS             126152..127333
                     /locus_tag="PLW53_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD35972.1"
                     /translation="MIEDLDKALSRAQDVLASPESIRRICISGKAKGKQPEQVRIDIR
                     PVALKAGLHWQVVSHDGKRDTTKNLALGELSLARLFNLGYSNILIESTSQEINLRLTK
                     SGDAQFSTKRVELDAAELTHDRTKERLLSADDEIFIELGISDQNGKLKPSRSDKFIQV
                     QEFLKILSHSLNEKRDKNQELKVIDLGCGHAYLTLAAHKYLSKQGYKVKTLGIDERQD
                     SRKRNIALVEKLKMSKEITFQATKIANLELANFDIAIALHACDTASDDAISWAVKSGV
                     EMILVAPCCHHDIQRQMKEAPAPWNIATRHGIINERVGDILTDSIRAGVLKILGYRTD
                     IIEFVAGEHTPRNLMIRAFKTGAPAQRADIAELEQIITQWKIEPALMVRLKEELSVRL
                     G"
     gene            127333..128703
                     /gene="argG"
                     /locus_tag="PLW53_00675"
     CDS             127333..128703
                     /gene="argG"
                     /locus_tag="PLW53_00675"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF003779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRD35973.1"
                     /translation="MSSKQSSGKVLASLPVGQKVGIAFSGGLDTSVAVAWMRQKGALP
                     CAYTADLGQYDESNIESIASRAKEYGAEIARLIDCKNSLVEEGLAALASGAFHIRSAG
                     KIYFNTTPLGRAVTGTLLVRAMLEDNVLIWGDGSTYKGNDIERFYRYGLLANPELKIY
                     KPWLDSDFVAELGGRKEMSDWLKSHNLPYRDSAEKAYSTDANILGATHEAKKLEELSS
                     SIEIVEPIMGVKFWDQAVAIAQEDVKITFKSGRPVAINNKDFSDVVELMRQANLIGGR
                     HGLGMSDQIENRIIEAKSRGIYEAPGMALLFIAYERLLSAVHNEETLANYYQSGRKLG
                     RLLYEGRWLDPQSLMLRESLTRWVASAVSGEVVLRLRRGDDYSIIDTKGENFSYHPEK
                     LSMERTQAAAFGPEDRIGQLTMRNLDIADTRQKLEMYRDQGQIGKGNFDLVEIENQEK
                     KETKGK"
     gene            128704..130350
                     /locus_tag="PLW53_00680"
     CDS             128704..130350
                     /locus_tag="PLW53_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRD35974.1"
                     /translation="MTTSAITKILDNFLEEGIKLSPIGATMLGVPGFDDQLDDLSMAG
                     NEQRAELTRKTLAAIKNETPINEFDRIAKDVAVERLTSELNLNDTFEARILFNLISSP
                     VTSIRQVFEMMKPDQAETVSNVTKRLNGIETAFAGWKSSLEYVAAKGKFNSRRQVLAT
                     AGQLETFSKGAFTAVAKKFNPAGDNQALLAAAKSAEAACASLSSWMRDSYAPKCPAVD
                     GVGEERYKMWARHFTGANLDLRDTYEWGIKQLEMINERMWSAAKKLYPDAKTLREVAD
                     RLEADPRYTVVGEEELLKKLKEFIAAAVERLDGKEFDIDPRIRNCEARLAPPGGASAA
                     YYMGPSEDLSRPGTTWYPTMGRKTFGWWHIVSTWYHEAVPGHHLQVATTKINTDRLNR
                     FQRNRVWVSGYGEGWALYAERLMDELGAFEDPGYEMGYLSAQGLRAARIVVDIGMHCG
                     YKDFNGEIWNAESAFKLLHERALLDEISARSEVDRYLGWPGQAISYKVGERFMMEARE
                     DAKKRLGATFNLKKFHSYVLNLGPMGLDPFKAEMAAWDGK"
     regulatory      130370..130497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            130560..131585
                     /gene="ribD"
                     /locus_tag="PLW53_00685"
     CDS             130560..131585
                     /gene="ribD"
                     /locus_tag="PLW53_00685"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRD35975.1"
                     /translation="MINAESAMRRANEISKLGLGKSSPNPIVGAVIVGNDGQIAGEGF
                     HHSQDGGKHAEVIAISRAGQRANGATLFVTLEPCNHMGKTPPCTEAIIAAGIKKVVFA
                     VSDPNPIAHGGAQALADAGIIVEQGLLKDEVAFTNRAWLKKIESSHPYITIKIAASLD
                     GKVAALDGSSKWITSEISRSDVAILRSQCDAIVTGTGTVLADDPSLTVRNIKRENFEF
                     KPVRVILGKREIPLDSKVLDKSAETIQIKGNDLGELMELAKERAWNQILIEAGPTLTS
                     AFLKAGLFDEVFLYQAPTLLGSGLDFASQLEIKTLNQRLDLELLSADLLGDKEKNLRL
                     HLLAVSK"
     gene            131585..132178
                     /locus_tag="PLW53_00690"
     CDS             131585..132178
                     /locus_tag="PLW53_00690"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRD35976.1"
                     /translation="MFTGLVADKAKVISIARGAESAVIQIESVLTPEIKVGDSVSING
                     TCLTAIELSSNTFKADVMIQTLKVTSLGKLSEGDFVNLELATRADSRLGGHIVQGHVD
                     GIGSVIENSPGEKWNKFVVRVPKNLTKYIVNQGSIAIDGVSLTVGEISDDLLTLWLIP
                     ETLERTNLFQKSSGDVVNIEVDVIAKYVERLMNRGQS"
     gene            132175..133383
                     /gene="ribB"
                     /locus_tag="PLW53_00695"
     CDS             132175..133383
                     /gene="ribB"
                     /locus_tag="PLW53_00695"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRD35977.1"
                     /translation="MTLSLDKTLEAFRDGGFVIVTDDADRENEGDLFLLASAATTEKI
                     GFMIRYTSGVICVAMTEERSRQLHLPLMVKQNQDTKRTAFTVTVDAKHEITTGISAKE
                     RANTIRALADLSSTAEDFIRPGHIFPLIAHEGGLAARRGHTEAIVEMCKLVGENQVGV
                     ISELVNEDGSMMRGENLTRFASEQGIPILSIAQLMSALEGSNKLDSQKEFHLNWAELP
                     RSSSGQIKQWQIATFLGRGGVDHALLKFGILNSEKPLLVRIHSECLTGDVLGSLRCDC
                     GSQLERAMNEIESAGSGLIIYLRDQEGRGIGLSEKIKAYQLQDQGLDTVDANLALGHN
                     IDERDFGDAAEILNSLKINSVVLLSNNPEKLETLTSSGIKASLQILSGDINTFNEKYV
                     ATKKERLGHK"
     gene            133383..133868
                     /gene="ribH"
                     /locus_tag="PLW53_00700"
     CDS             133383..133868
                     /gene="ribH"
                     /locus_tag="PLW53_00700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRD35978.1"
                     /translation="MSGVVPKVSLGDLGQYRVAIISASWHEKICADLIAGARKALDLA
                     KIKPEPVIYVPGSFELPLAAQLALDSGFDAAVVLGVVLRGETPHFDYVCQGVTQGIMQ
                     VSLTRSKPIGFGVLTVDTVEQAIARSGITGSKEDKGFDSAVAALDLLRVKSELPVLRK
                     S"
     gene            complement(133845..135344)
                     /locus_tag="PLW53_00705"
     CDS             complement(133845..135344)
                     /locus_tag="PLW53_00705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRD35979.1"
                     /translation="MSEVKRITHHINGAYWTESATRSGDVYNPATGRVTAKVDFATPE
                     LIDHGVKVAKKAQEQWAQASLAKRVQVLFAFREILNARKEEVAKLITAEHGKVLSDAL
                     GEVTRGLEVVEFACGIPHLLKGAYSEGASTNIDVYSIRQPLGVCAIISPFNFPAMVPM
                     WFFPIAIAAGNAVIIKPSEKDPSAINLVAEFWKEAGLPDGVFNVIHGDKVAVDSLLSH
                     PDVASISFVGSTPIARYIYENGTKNGKRVQALGGAKNHMLVLPDADLDLAADAAVNAG
                     FGSAGERCMAISALVVVEPVADELIKKISERMTNLKTGDGSAGSDMGPLVTAVHRDKV
                     ASYIDAGVSAGAKLVVDGRNPKVSGAAEGFWLGPTLFDQVTPEMSIYKDEIFGPVLSV
                     IRVSSYDQGLELINKHQYGNGTAIFTNDGGAARRFQNEVQVGMIGINVPIPVPTAYYS
                     FGGWKNSLFGDTHAHGMEGVHFFTRGKVVTSRWLDPSHGGINLGFPQNR"
     gene            complement(135347..136702)
                     /locus_tag="PLW53_00710"
     CDS             complement(135347..136702)
                     /locus_tag="PLW53_00710"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD35980.1"
                     /translation="MAEFIPNPARGKEVYDLDRKYVFHSWSAQGQITPLPIAGGSGSY
                     FWDFDGKKYLDFSCQLVFTNIGFQHPKVIDAIKRQADLLTTIAPQAANEARGEAASRI
                     VSLASPNHEKVFFTNGGADGIENAIRMARIHTSRHKILSFYRSYHGNTTSAISATGDP
                     RRWPNEFGYGHVHFFGPYLYRSAFWAKSEEEECQRALEHLESVIEFEGPKTIAAILIE
                     SVVGTAGVLIPPKGYLEGVRRLCDKYGILWIADEVMSGFGRTGKWFAYQHSSAHPDLI
                     VFAKGVNSGYVPLGGVVISKEIAETFKDRVFPGGLTYSGHPLACAAAVATIDAMKDEK
                     IVEAAATIGEKVIGPGLRDLASKHPVIGEVRGQGTFWALDLVTDRASRAPLAPYGGSS
                     PAMNELVGECKKLGLMPFTNFNRMHVVPPCNITEAEFNEGLAILDKAFTQIDKYYTGA
                     K"
     gene            complement(136754..137650)
                     /locus_tag="PLW53_00715"
     CDS             complement(136754..137650)
                     /locus_tag="PLW53_00715"
                     /inference="COORDINATES: protein motif:HMM:NF001134.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRD35981.1"
                     /translation="MLPKIILYYGFAPVADPEALRLWQNTLAQSLNLKGRIIISRHGI
                     NGTLGGDMDDLKKYVRATKQYPGFKKIDFKWSQGTGSDFPKLSVKVRDELVTFGIPEN
                     LEVNEKGIIGGGEHLSPEEVNELAKQLGEDLVFFDGRNAYEAKVGRFKDAVIPDIKTT
                     KDFVAELDSGKYDYLKEKTVVTYCTGGIRCEILTKVMKERGFDKVYQIAGGIVRYGEK
                     YADQGLWQGSLYTFDKRMVIDFSESTKLIGKCDKCSEPTKQFHNCANVYCHNLFLLCE
                     KCGKDEKNLVCIHQGVVTSNNH"
     gene            137715..138134
                     /locus_tag="PLW53_00720"
     CDS             137715..138134
                     /locus_tag="PLW53_00720"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HRD35982.1"
                     /translation="MASKSAKSKQVAKRKTVSPRPSSPAQRQSEPAIAKREDRIGWYS
                     LYIYAVCLVTLLTCLFSLVSVVRGIVDALWPDPGYFDPYSVPKDSALSPDQIKENLTE
                     NNQRQALKGIVNSLTTLIIAGPIYLMHWRLAKRSRAI"
     gene            complement(138154..138687)
                     /locus_tag="PLW53_00725"
     CDS             complement(138154..138687)
                     /locus_tag="PLW53_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HRD35983.1"
                     /translation="MNYHLARGNFEFVSDTLKTVMKRLLGLTRYAVVIPALASILGAL
                     LLMAQGSFAMVLAVIDSLESQYGLKETIVEVLTAIDAILLGTVLLVIGYGLYELFIDT
                     DIDVPLWLRVEDLDDLKSKLIGVVVAIIAVVYVGVFVDSNRGDDVIAYGVGAGALVAG
                     LALFAFATGKAGKKSRE"
     gene            complement(138716..138788)
                     /locus_tag="PLW53_00730"
     tRNA            complement(138716..138788)
                     /locus_tag="PLW53_00730"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138753..138755),aa:Lys,seq:ctt)
     gene            138879..139646
                     /locus_tag="PLW53_00735"
     CDS             138879..139646
                     /locus_tag="PLW53_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HRD35984.1"
                     /translation="MKSNWQKAIFSLLKSKKELKHRAAFTSQETLIVLIASFALTSFS
                     LVSPSSKYQIEILELASANGAEFDPIKALETLAVKGRAPKTGYSRSAFGPAWADVDRN
                     GCDTRNDILKRDLTQVTFRAKTKDCVVESGVLLDPFSGESINFQRGEKTSTLVQIDHV
                     VALSNAWQTGIFKADLATRKNFANDPLNLLAVKGSLNSQKGDGDAATWLPPNKQFRCD
                     YVSRQVEVKVKYGLWVTRAEKDATLRILSNPPCLKQN"
     gene            complement(139651..140982)
                     /locus_tag="PLW53_00740"
     CDS             complement(139651..140982)
                     /locus_tag="PLW53_00740"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRD35985.1"
                     /translation="MSEELPQGTSKRSAKMASIPLSLAGRGAIGFGRQLIGQSPDFAF
                     ADLQEKTAEQIFKVLGELKGGAMKFGQALSVFEAALPEDIAKPYRETLVKLQEAAPPL
                     PARVVHKVLAKELGEHWRDNFAEFNDTPAASASIGQVHKGIWKDGRAVAVKIQYPGAK
                     EALISDLNQIQRFAKIFQLLLPGVEMKPLLEELKARIIEEVDYRYEASAQSACFEAYK
                     GDSDIAIPEVIMATDRVLVSQWLEGTPLSKVITDGTQEERNSAGIRLARFHFTAPMRA
                     GLLHADPHPGNFRVLKDGRLGVLDFGACNRLPNGFPEPFKRLLRNALEGDAIALYNGF
                     KEDGFILADVEVSPELVLDYLLPLVEPLRTDYFAYSRDWLRTQSVRVGDPRNPTAKIG
                     FQLNLPPEYVLIHRVTLGTTGIFCQLRAEGNFRDEALSWFPEITPSHLQKS"
     gene            complement(141025..142134)
                     /locus_tag="PLW53_00745"
     CDS             complement(141025..142134)
                     /locus_tag="PLW53_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35986.1"
                     /translation="MALDDRPLMESRPAIKSALSIWLSPNGAQVFIGSPYKSICLPSL
                     WFGISTRLLLESFNGQLSLEQISKQCALTISQLQALVDELQAHDLIDLERTAISYLKR
                     YDPRVKAITPVTDLEDFNEDLAAQSFIKRMEIESEAASLEKGDIDGGRSAVVRRRDFS
                     ILIFGYGKIVNSLVGLLSASGFTKVLVINRLQQRSSALKILESDLSGGYISRADIGAS
                     RKEVLAKARSSSALFAEATPVIYRPRLIISVGRPTPDVLQRWISEGSNHLIIEPHSSA
                     EVRIGPYVIPGKSPCYRCLQISSEEKLSSVERKEVGSALALVTAAITCLEVISLASSG
                     ISHFLGKSLIYSNSNFHNPKLEKWSINPGCGCNWR"
     gene            complement(142290..142448)
                     /locus_tag="PLW53_00750"
     CDS             complement(142290..142448)
                     /locus_tag="PLW53_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HRD35987.1"
                     /translation="MAETYKGEAYCVKCKAKREFEGTVKVSESGRRMAQGICPTCGTK
                     VNRILGKA"
     gene            complement(142560..143171)
                     /locus_tag="PLW53_00755"
     CDS             complement(142560..143171)
                     /locus_tag="PLW53_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRD35988.1"
                     /translation="MENFIQDEIFNDNLPQMRDDGQRAASVIKIPPTPSSNGAEVKVI
                     RSTRRKKSISAYRQAGAIVISVPARLSKSEVRQVIPEMVSKVLSLEAREKIDEEDLYK
                     RALELLAKYLPECPVRPVSVTWRSMSERWGSCTTVERSIRISDRLNGAPGYVLDYLLV
                     HELIHLMIADHGPDFEALLARFELGKEASAYLEGYEAGVRAGN"
     gene            complement(143375..144700)
                     /locus_tag="PLW53_00760"
     CDS             complement(143375..144700)
                     /locus_tag="PLW53_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRD35989.1"
                     /translation="MPGFGFTPDNGDEDESKGNNPFGFENFGEIFKQFSGMGLNLPAL
                     MASLSGQASPASLSKEMIRDISRKYLSAHGEVPVTVEDLAQIQEAINIADLWINEATT
                     FPALPLTDSIALSRRDWIDSTLNGWQELTKPLVDSMSAALSSMLDQGLDGQGEDNGIS
                     IPGFGPMSISKSSINAIMATFMSSLISTQLGQSIGALSTTVTGANDVALPLSKDIKAQ
                     LLPQNVRAWGSGLEIQEAEIRIYLALREIAAARLFKQSPWLSGYIGEVISAYGSGIRI
                     DINAITQSAQDAMDSGAIDPSNPESMASALSGGLFTPETSASQSAALEKLETLLALIE
                     GWIDAVVAKAATDRLPSLIKLRESQARRRATNSPTQQLFATLLGLEVSPRLTREAISF
                     WEKIEKIDSISARDKIWEEAFLLPTASQLLDPEGFSKSRKIPDDLSGLI"
     gene            144869..145093
                     /locus_tag="PLW53_00765"
     CDS             144869..145093
                     /locus_tag="PLW53_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35990.1"
                     /translation="MSEVMAIKWAAESASAPLSALIWWFIPLTAAVGAAGYVIWVTKF
                     QKKYENKTERSVGRFQSFQKSFKSSESDKE"
     gene            145099..146166
                     /locus_tag="PLW53_00770"
     CDS             145099..146166
                     /locus_tag="PLW53_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD35991.1"
                     /translation="MRNISPLRYRWSFPVKFFFYLLTIATLLAPIPFIFFKPGVPDKV
                     TGKLIQVNDFKTYPVNGELYITSILVTNPDSPVFGAETIVNWAIGANVVLPRDAVYPP
                     VKPAQVIQRDSRSEMESSKITATAAALRYLGYDFIELYFISAIRDYSNAKEKFKVGDF
                     IKEIDGKVIGEIEEIRSSYAEKDIGDPLLIAVDRENSKGELERITDEIILVENQEVVN
                     EDGSKRPAIGILVGTTARFPIDIDFNIRGVGGPSAGLIFAVGIIEKLTDEDLLRGRKV
                     AGTGTITPSGQVGAIGGIEEKMIGASRIGATVFIAPRENCPDIKNIPAGLKVIPVSTL
                     AEAIAALRAPDSFVPRSCSNS"
     gene            146195..149083
                     /locus_tag="PLW53_00775"
     CDS             146195..149083
                     /locus_tag="PLW53_00775"
                     /inference="COORDINATES: protein motif:HMM:NF000825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRD35992.1"
                     /translation="MTFGDNPDNPFRNFRFPSRNLNGGQRPKIGALPLTVIILTVLAV
                     ILVSLSGFYADFLWFRSVDYSQVWTTLLTTRITLFFIFGLLTSLIITANIYIAYKRRP
                     IYVPVTVEADNLERYRAQLEPLRKVGLIGVAVALFYFAGMAGTRLWESWLLFRNATEF
                     GVVDPQFGRDISFFAFELPFYQSIVGWAISTLILATIASLAVHYVYGGIRPQVRQDRT
                     TVAARVQLSVLLGFIVAIKAVAYYLDRFALATSDEGIITGLTYTDVNALLPAKSILTG
                     IAALCSLLFFANIIRRSWVLPAAGVALLGISSFLIAGVYPGLIQQFQVKPSESSREAP
                     YIQRNIEGTRAAYGLDKVEVKDYSAIVDTSAGQLADDAATISNIRLMDPNVLSATFRQ
                     LQQIKPYYTFNESLDIDRYTIDGVTRDMVVAVREINIDGNPNRNWINDHLVYTHGFGF
                     VGSFGNTQDIDGKPVFSVGDIPPKGLLGDFQPRIYFGENNPEYSIIGGTTDGEAVEFD
                     YPDDASANGQKNYTYTGKGGVPMGSIFSRLLFAIKYQEQRILLSNLINADTKIIFDRE
                     PRQRVAKVAPWLKLDGDPYPAIVDNRIQWIIDGYTTSSGYPYSRTVDVSGATTDALNI
                     NNNPLTAIPNSTINYIRNSVKATVDAYDGTVTLYAWDEKDPVLASWMKAFPGIVKAKS
                     EMSKDLVSHVRYPEDLFRVQRDVLSLYHVKNANAFYGGQDFWRVPRDPSTLGANAGAQ
                     PPYYYTLQLPGEKKASFALTTPFVPRGGRENLSAFAVVNSDPGDDYGKFTVLQLQRST
                     NVAGPSQVASNFEANPTVALSLSLLRQGGSDVVLGNLLTLPVGGGLLYVQPVYVRATA
                     NTAAYPLLQKVLVSFGEKIGFDDTLKGALDQVFGGNSGSTNLPPASGGSTGSNTGSNN
                     DLASALASAQAALADAQAALAKGDFAAYGKAQDRLKAAIAAAVAAQNR"
     gene            149130..149206
                     /locus_tag="PLW53_00780"
     tRNA            149130..149206
                     /locus_tag="PLW53_00780"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:149164..149166,aa:Met,seq:cat)
     gene            complement(149266..150258)
                     /locus_tag="PLW53_00785"
     CDS             complement(149266..150258)
                     /locus_tag="PLW53_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRD35993.1"
                     /translation="MNLSNSIWIGTVAFIVGLLIFDLLASSRKAHDVSFKEASFWSVF
                     YIAVALIFGAWVWIEFGDQFGKEYFAAYVVEKSLSVDNLFVFVIILANFAVPSIYHQR
                     VLMVGIVLALIMRAIFIALGAAALATFSFTFVIFGAILLWTGVKLFQHWDEDPDLNDN
                     AIVRYARKKFAFTDQYHGTKMFIKENGKRYATPLLLVMVAIGSTDLLFALDSIPATFG
                     VTQEPFLVFAANAFALLGLRALYFLIKGLLQRLIYLSLGLAFILIFIGAKLILLWAHE
                     IWVEVPKVSTNTSLLVICLILIVATVASLIKSKRDPSAIAHAGRMSGPKKEKDK"
     gene            complement(150255..152513)
                     /locus_tag="PLW53_00790"
     CDS             complement(150255..152513)
                     /locus_tag="PLW53_00790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRD35994.1"
                     /translation="MSDTQVVDLEQIDLSISGMTCSSCVGRVEKSLNEVPGAKASVNL
                     ATDSAHILIPRGANPKDLIAAVVKAGYSAKLRGDGYESFSHSRAMGFRLIAAILLTIP
                     VITLSMWHQLHMQVDKFILTQLDSLNIAAPLYSPTGWLVISLSAPVVLLIGWPIHRAA
                     IKNITHPSMDNLISLGSLTALIWSIYANSTGNGDIYSEVGASVVTFIVLGRYLESRAK
                     RKAGSSLAHLLSLTPKQVIIIRGGQEITAPIANLVIGDHCLVKAGQAIPTDGIVIQGS
                     SAIDNSLLTGESLPVDVQAGASVIAGAINLNSPIVIEAKRVGSDTELARITKMVLTAQ
                     GEKAPIQRLADRISNVFVPIVIALAIATFATWYGLGNSLTDSITASVAVLIIACPCAL
                     GLATPVALLVASGKGATNGIILRKSSSLEIATKIDTAIFDKTGTLTTGVMKVQNITIA
                     DLGAAAPKISTSDLLIAVHSLEHESSHPIAVAISKSLREQGIARKKFTDYVESAGMGI
                     AARMQFGEAQMPVLIGTPASIRRATVSLHPQLEDAISSALSKGNSVALVAVDGVAMAL
                     FEVGDSLRVDARQTIAALKSRNIDSWLISGDSQEAATQIADQVGIALSNVIAQATPDQ
                     KIAKVKELRSSGRKVLMIGDGINDAAALSEADLSMALGTGTDTAISSADITLMRPTLA
                     TAISALNLSAKTLRIIRVNLGWAFFYNVIGIPIAALGLLNPMYAGGAMAVSSLLVVLN
                     SLRINRSATLSA"
     gene            complement(152510..153319)
                     /locus_tag="PLW53_00795"
     CDS             complement(152510..153319)
                     /locus_tag="PLW53_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD35995.1"
                     /translation="MTYPGDLLGDIELLKYLADLSDEISSSRFLAQDLKIESKPDLSP
                     VTDADRAVEEKIREVLKAQRPNDKIVGEEFGSAEDIQPLDRYWVIDPIDGTKNFLRGL
                     PIWATLIGLVHRNENGQDRVIAGMVSSPALFRRWYAAEGFGAFTEVNSGAAIQIKASN
                     VKEISDASISFSDLVGWGPRKNSYLAFMDKAWRARGIGDFWSHMLVAEGAVDIAAEPS
                     LALWDMAAVAIIVTESGGRFSDLENVAGPFGKSGVSTNGHLHQAFISAVNS"
     gene            complement(153325..154305)
                     /gene="rsgA"
                     /locus_tag="PLW53_00800"
     CDS             complement(153325..154305)
                     /gene="rsgA"
                     /locus_tag="PLW53_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRD35996.1"
                     /translation="MVRRRPDESDIRIRPAKSTRRRSKDRPSHSDSIAAQVITVDRGR
                     TLCRIASGKLVSAMKARELGKNAVVVGDLVNIVGDVSGDAGSLARIVAVQPRRNSLSR
                     TIDDAGAFEKTIAANLDQMVIVAASADPAPRQGFIDRCLAVAFDQGIKPIIVMSKADL
                     ADPDEFLIAYKDLEVDYFKLKRGDDLTQLHKALEAKVSVLIGHSGVGKSTLVNALLGI
                     EKRDTGDVNSTTGRGRHTSSSAISFELPNGGWIIDTPGVRSFGLEHIDKSRVIASFTE
                     FASVIANCPKNCSHDEATCALNDYVKTSPQASSRLASLQRLLDSSNQEII"
     gene            complement(154298..155560)
                     /gene="aroA"
                     /locus_tag="PLW53_00805"
     CDS             complement(154298..155560)
                     /gene="aroA"
                     /locus_tag="PLW53_00805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRD35997.1"
                     /translation="MWSAPFRASKPISGAINIPGSKSVTNRALVLSALASSPSTLRRG
                     LRSRDTDLMIKALSSLGVKIEIEEDLWKITPAPLMGPAGIDVGNAGTVMRFLPPLAAL
                     AKGLISFDGDPRSHQRPLGPVIKALESLGVSIEHQGRYSLPLVINGAGYINGGEVEID
                     ASASSQFLSSLLLVAPLMKQGLKIKNVGKNLPSKPHIEMTISMLAQYGAVVDTSIANQ
                     WSVKPTKLSGVDLVIEPDLSNAAVFMAAPLVCGGEVIIKDWPRKTTQPGDQLRQIFSE
                     MGGVIDFVDQGLRVRSDGQVRGIDIDLGDVGELTPVIAALACFANSPSYLRGIGHLRL
                     HETDRLSALKNELTALGAEVIEEESALRILPKPMKAGVFHTYEDHRLATAGALIGLAV
                     DGVEVENIETTKKTITDFPALWSQLLNG"
     gene            155604..156311
                     /locus_tag="PLW53_00810"
     CDS             155604..156311
                     /locus_tag="PLW53_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRD35998.1"
                     /translation="MCGRYALSAMISDIAEEFSTNSAPGISLPADWNIKPTNDVYIIK
                     NQAIEIASWGMIAHWSKSDDEATKSQSSAINARSESVHEKPTFKSAFRSNRCLLPASG
                     YYEWASELGKYKTKQPIYVSRDDEKLLAFSGIFQSWTSPSGKVIQSVAIITRQAVGQL
                     AQVHSRMPVFLPKERWQDWMNPKINDVDKIRALMDVPTPDANLRFHPVSSAVNSITGS
                     GPGLIEPIELGEPETLF"
     gene            156350..157012
                     /locus_tag="PLW53_00815"
     CDS             156350..157012
                     /locus_tag="PLW53_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD35999.1"
                     /translation="MAITLDLMPTEISNQGSRDAGELVRVDREKESAEQRRKRFEADA
                     LQYMNQLYSAALRYTKNPSDAQDLVQDTYAKAYVSFHQFEPGTNLKAWLYRILTTTFI
                     NTYRKDQRRPQLSDQELEDWQIADASSHTSDQGKSAEDVVLENLPDSDIKRALAEIPE
                     EFRIAVYLADVEGFSYKEIAEIVGVPTGTVMSRLHRGRKQLREKLTDYARELGYVKGG
                     KK"
     gene            157009..157383
                     /locus_tag="PLW53_00820"
     CDS             157009..157383
                     /locus_tag="PLW53_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36000.1"
                     /translation="MSIPCDKCCESLVLFIDNEIDDSATFNEIAFHLQDCQGCRGEME
                     SQRAALNLIQSLLTRSCCESAPSSLQEQIALRLAELAGQEMLPTSQSEIITQYRRTEI
                     TIDGQTSIEIETSHEIRRDFPF"
     gene            157412..157822
                     /locus_tag="PLW53_00825"
     CDS             157412..157822
                     /locus_tag="PLW53_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36001.1"
                     /translation="MQAELILGAVGISQMVVRPGDTALAHGSGDMPILSSSFLITLME
                     SACNSAIAEFLENGETTTLTDMELEIHDALGIGSEIQGSATCVSYEEGILTFACEIHD
                     GSRLIAASVMRRALVERVTFLARTAALSLISEGK"
     gene            complement(157831..161484)
                     /locus_tag="PLW53_00830"
     CDS             complement(157831..161484)
                     /locus_tag="PLW53_00830"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HRD36002.1"
                     /translation="MTPENKFGGSFGSNEWLVDEMYERYLADPNSVEANWQEFFSGYK
                     PATNGAASSVAKAATPPIPKRQQVKQAPAQEIQNTPPPIQEVVRANPAPIPTPAEAVK
                     KSAPQIATPTAARVEPLRGVSARVVTSMEASLTVPTATSVRAIPAKLMIDNRTVINNH
                     LKRARGGKVSFTHLIGYAMIKALRENPEMNTFFTELDGKPAIGYPDHINLGIAIDLTK
                     EDGSRQLLVPSIKGCEGLDFGNFWASYEALVKKARSGALSVEDFSGTTVSLTNPGTLG
                     TVHSVPRLVTGQGLILGVGAMDYPAEFQGASEETIANLAISKVITLTSTYDHRIIQGA
                     QSGDFLKKIHEILLGADNFYEEIFAALRIPYVPITWHNDIPEGKEQLNKAARLQQLIQ
                     AYRTSGHLMADTDPLEYQQRSHPDLDVITHGLTLWDLDREIATGGFSGRPYAKMRNVL
                     GILRDSYCRSIGIEYMYIDSPEERKWIQSQVEVGAPFFPREEQLRILRKLNSAEAFET
                     FLHTKFVGQKRFSLEGGESVIPILDAIARYAAKAKLDEVCIGMPHRGRLNVLANVAGK
                     NYGQIFQEFEGHYQENEVQGSGDVKYHLGTYGDFITESGDQTKIYLAANPSHLEAVNP
                     VLEGIVRAKQDKKNVRDIYSVLPVLIHGDAAFAGQGVVSETLNMSQLRGYRTGGTIHV
                     VINNQVGFTTSPDSARTSRYCTDIAKMIQAPVFHVNGDDPEACVRVARVAFEYRQSFN
                     KDVIIDMVCYRRRGHNEGDEPSFTQPRMYKLIDAKRSTRVLYAEALVGRGDITQEESD
                     EIAKEFQGQLEAIFNMVHDKLPEKPAFNKLEELDPLKANTAISEEMARKIAATQVATP
                     QGFKVHPKLAPQLQRRVTMLDDGTIDWSGGEMLALGSLLLEGHTVRLAGQDVGRGTFS
                     NRHAVIRDYENGSEWIPLRELISGSTHLFVIDSLLSEYAALGFEYGYSLEREDALVLW
                     EAQFGDFANGAQTIIDEFISSSLQKWGERSSLVMLLPHGYEGQGPDHSSGRIERFLTL
                     AAENNMTIAQPSSAASYFHLLRWHVKNPARRPLIVFEPKSMLRLKAAAAQLTDFTTGT
                     FTPVLSDPAITSASKIVLCSGKIYWELVAERAKRSDSSTAIIRLEQLYPLAIDQILAE
                     LSKAPNAKLVWVQDEPANQGPWPFISSNLQEALGARKLNRVSRKSAASPATGSIYMHE
                     EEQKALLAEVFAS"
     gene            161483..162298
                     /locus_tag="PLW53_00835"
     CDS             161483..162298
                     /locus_tag="PLW53_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD36003.1"
                     /translation="MPATESKPIFVALCLPTAKWRSWPHFGDAKSASLGQAYEMALAF
                     VTGASRGLGYEVAKALKARGDQVIGISKDRQRAEAASSELGIAFELMDCQQISQVRSV
                     SQELLAKYGTPEILVLAHGVMSEKMAKTLKTNDQEWARVLDINLNSVFTLVNSIAAPM
                     AEARNGRVIIFSACLGRMSGPGNAGGLAPYRISKAGVNALVRNLAHETNHGARGFLVD
                     AICPNHSRTDMGGADAPRSAAEGAATAIWLATRTFNPGDQTGLLWEDQEVVPW"
     gene            complement(162277..162654)
                     /locus_tag="PLW53_00840"
     CDS             complement(162277..162654)
                     /locus_tag="PLW53_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36004.1"
                     /translation="MKLFGSKVYLIAATLVRLEALFLAGLASYLAIRTATSKVEELDA
                     ILAEIIFLSIASTGLFFAGKGFIAKRNFARAPTVLANLIAIGVAYYMIDGERDLLGIG
                     LGSFALVTLLAALAAIPHQGTTS"
     gene            162657..163109
                     /locus_tag="PLW53_00845"
     CDS             162657..163109
                     /locus_tag="PLW53_00845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRD36005.1"
                     /translation="MALRIGDEIALVIIKKASKAWLIMTSFDSSSFDRLTGLMTPVYF
                     YESLSRLRSWAQRSDNPVTLIAINLKDLSDDQLLEVARELNSELRGGDLLARMAPKRF
                     LLALVADQLGARQFLFRITNKIKEASNYQLIELSPSKDLAEALSEIDI"
     gene            complement(163114..163665)
                     /gene="orn"
                     /locus_tag="PLW53_00850"
     CDS             complement(163114..163665)
                     /gene="orn"
                     /locus_tag="PLW53_00850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRD36006.1"
                     /translation="MAQENPALIWIDCEMTGLNLASDALIEIAVLVTDSELNLLGDGI
                     DLVIATTPEKLASMSDEVKRMHSESGLIEKVANGVSLSIAEERILEYLKQYTAEGKSP
                     LAGNSVGMDRNFIARDMPLLNSFLHYRTIDVSSIKELAKRWYPKAYFQAPKKSGNHRA
                     LGDIQDSIAELAYYRSAIFVSDK"
     gene            163805..164932
                     /locus_tag="PLW53_00855"
     CDS             163805..164932
                     /locus_tag="PLW53_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD36007.1"
                     /translation="MFSKKRILAMFTATGLALSLMSAPAFAKEADGPVTNLGKCSSRN
                     KIAYDAKGIEYKCSIAGGGLKWKKTGGKQGAGASTSTSTIDTKKYANVKGSIKIDGSS
                     TVAPLTGVAAEAFQKISQTQITVGISGTGGGQTRFCKGELDIANASAAYSATQRKQCE
                     DAGIKFTELRLATDALSVVVNPKNTWAKCLTVAELKKIWEPGSKVNYWNQVRADFPRV
                     KMPLFGAGTDSGTFEYFTEQINGRPAKRSRVDYTPTEDDNVTVNGVKRSTGAMGYYGF
                     SYYKENTDINKAIAIDGGKGCAEPSLENVLSGAYTPLARPLFIYVNNDQVKKNPAIIP
                     FLEFYAADLKNLSEKAKFLPLTAEQTKKLEADLAELRKLAN"
     gene            164991..165935
                     /gene="pstC"
                     /locus_tag="PLW53_00860"
     CDS             164991..165935
                     /gene="pstC"
                     /locus_tag="PLW53_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRD36008.1"
                     /translation="MSATAQSGRAGMSLAPSTHLRRRNLIQLLITRFMASATLVSVLV
                     TVGIVLSLIPTTFRLLKEITTQIFGTVWAPLFTPSQYGMAPLLTGTFIIVSIAMVIAI
                     PIGLASAIFLSDYATFNLRRIVKPILEILAGIPTVVLGLFGLYVVNPEFVEKYWPIGE
                     PLAYTALGAGLMTGILIIPIIASVADDALSSVPRAMREAAYALGATRREVATKVVLNA
                     GLSGVVAALILAFARAFGETTIALMVAGSYPKLSANPGDPMQTMASFIGFAGIGDAEV
                     GSSAYNTIFLVGTVLFTVTLILNILGNRFIARFREVYE"
     gene            165935..166825
                     /gene="pstA"
                     /locus_tag="PLW53_00865"
     CDS             165935..166825
                     /gene="pstA"
                     /locus_tag="PLW53_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRD36009.1"
                     /translation="MATPSTSLESRVSLDRRIKDFTFTTFLSISIVASLAFIFYIIYR
                     LYNNAGFLLSWDLVRFFPSYDIDTAGFQSSIMGSIWVVGFATLFAVPIGVMTALYLEE
                     FAGKRNRFTALLELNIQNLAGVPAVVFGLIGLAFIARGPLSWGFTVGSAAIVLAMLIL
                     PVIIIITREAIRAVPPSYKEGALALGATHWQAVSKLVIPSAVPGTATGTILAVSRAIG
                     ESAPLLLLGALSFVTFNPTGLDSGFTILPLAIFKYASDSREEFQVLASAGSLILLAIL
                     FAMNLFAILLRDYTLRKRNI"
     gene            166825..>167230
                     /locus_tag="PLW53_00870"
     CDS             166825..>167230
                     /locus_tag="PLW53_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36010.1"
                     /translation="MSMIFRQSQANKVSKEEPKKGSSPMNSSATRKPSEPLKFVDPVF
                     TLKDVAVSYGDFVAVSDVNMQIPSHDITAFIGPSGCGKSTLLRTLNRTNDLIPGAKVH
                     GSLFYHDADLYHPSASAVEVRRRIGMVFQKPNP"
BASE COUNT        46837 a        35181 c        38494 g        46718 t
ORIGIN      
        1 cacggcaatt gattaatggg ttaattcata atggatttcc tcatcacaca gttgtagctt
       61 ggaaagacat acgagcagaa gtccgcagga tggcggaata tttggccatt ccgctaaccg
      121 agtggtaatc acatgattca ccacagaccc tcgaataagg agaaataaat gaaacaaaag
      181 atgaatgcaa aagcattcaa agctctgatt gcgatggctt ttgcaatcag cgcatctgtt
      241 gttccactct cccagagctt tgccgctggc gaatctcttg gaaagagctg tacaacagaa
      301 ggactaatga ctggaactaa gtcaacttct cttgtctgcg taaaaggtgg caacggaaag
      361 ctcacctggc agcgcgttaa gttggcatcc ggcacaggac gcccagtagc aaacttgaca
      421 ccaccaaaag gtgaaattga attctggcac tatcgtgtcg agcttgctga tcagagagct
      481 ctagtaaaaa tcattagcga ttttgaggct aagtatcctg gcaccaagat taagcaagta
      541 gttcgtgaca ccacgaccta taacaacaca gcacgtgttg caattcttgc taatccaaaa
      601 gcagcgttat ttgctacctc tcgcggtgcg atctttgatc aattcgctaa gagcgacatg
      661 atgctagatc tatctgatca gcgttatgtt aagcagaacg ttgttgctaa gggattaact
      721 gcaggtcagt atgaaggaaa gcaactcggt attccttatc aatacctctt taacaaccca
      781 atcgtcaata cagaaatttg ggctaaagag aagtggacca caccaaagaa tctaactggt
      841 tgggtcaact ggtgtaagga tgccaaggct aaaggatatg ttcctctagc ttggccagga
      901 gctacacgtg gacaagcagc gcagatttct aactctgctc tgatgaactc tgcaggcagc
      961 tacgacgagc tagcaaagaa tcttgctgat ctaaatagcg gcaagattga tctaacttca
     1021 aagtggtttg aaggcgttgc agacatttat gtcaagctaa gaaacgccgg ctgcttccca
     1081 gataacgcca caggtgttac tgaagctgca gcatacaacc tctttgcaac tggtaagtca
     1141 ccaattcttc ctatcggaac atttgcgatg ggttcaattg tcggactaaa tccaaaccta
     1201 aatggcaaga tggaacttat gtcaatggtc ttgactgatg gcaaagttgt cgcagaggga
     1261 attatgaata acacttacac cctaagtgtt aataagaatt cggctctacg cgaccagaag
     1321 attgccaatg cattcctttc ctatctattg actggaaaag ttgcagaaat ctatgcaaat
     1381 gaaactcttc agcacgttaa cgttctagat gttgaatata agaacgtcaa ccttctaaac
     1441 acagtttcat tccaggctaa gaacactatg ttggcacctc gcttcctact tctgaatgca
     1501 gccgtatctg atctaactca ggatgcgcta attcagattg ttggcggcaa gagcccagct
     1561 gatgttttgc ctgatttctc aaggcagatc aagcagaaat tggctggctg attagctcgc
     1621 taacaactac cggaaccgct cccacctcag gtgggagcgg tgctcggcct gctccaaaga
     1681 aacgcaagaa agacgctggc atctattact tgatgggcct ccctgctgcc atcatttttg
     1741 caggtttcta ttttgttcca tttatcgcaa atttaagata ctccctgacc agatgggatc
     1801 gaataacgga gccagagttt gttggattcc gaaatttcat taatttactt actaatgatg
     1861 atctctttta taaggtttta ggaaacaacc tacgctttac cttcttagtc gtactattcc
     1921 aaacgctttt ttcattgata tttgcaattt tccttgttaa gaatacaagg gccaatattg
     1981 cgcttagaac tcttttcttc tttcctacaa ttctctcatc tgtgtctgtg ggaatgatct
     2041 ggctctttat gtatgaccct aactttggag cgataaattt attcttcact cgcttggggc
     2101 ttgaaggctt tgctttaaat tggcttggaa gtgagagctc tgcactatat gcaataacct
     2161 ttactcaagt ctggtttcat accgggcaaa tgatggtgat atacatcgct ggactacagc
     2221 agatacctca agaactttat gaagcagctg aaatggatgg tgcttcacgc tggcagcaat
     2281 tcagaaatgt aacttggccg atgtctattc cgaccaccat tgttgttatg gcttacacca
     2341 ctattcaatc ctttagggca tttgacttaa tttttgcaat gacacaaggt ggcccaaata
     2401 attcatcaaa tatatttgca gtcttgatat atcagactgt ttttagtgag ctcagaatcg
     2461 gatatgccgc tacgcagtca atcttcttca taattgtctt ggtttttgtc accttattgc
     2521 agcgcaagtt cctcaatagt cggtatgaga aatagggggc tgaaatgata tataaagtaa
     2581 gcagaatcgt ttctctagtg attttctctg tcttaatcat aattcccagc cttattgttg
     2641 ttttcggatc aacaaagact gatagcgagg tctataacaa gcctttagct cttcctgaga
     2701 aatggtcact agataacttc agattcctct ttgaggtaag caatgttggt cgttcatttc
     2761 taaatagttt aattgttaca ggcttttctg taaccataac tttgatactt gcaagtatgt
     2821 gcgcctttgc tatttccagg atgattacag taactggtcg agttttattc tcattatttg
     2881 ctgtgggtct ggccattccc ggtcaggtca atctagtacc aatctttatt ctctttaacg
     2941 acctacaact aaccaataag ctatctggtc tagttttgat taacatcgta accacattac
     3001 cgatctctgt atttatcttg acagcatttt ttaaggagct tccaaaggag atgtatgaag
     3061 tctctgaagt tgatggtgca accccacttc gtgtttatcg ctcaattgca cttccgcttt
     3121 caaggccagc gatgggtgct actgcaatat tccttttcgt tataacgtgg aatgaattgc
     3181 ttttcccatt actactaatt accgataacg ataaacggac cttgccgctc tcattgcttt
     3241 cattccgtgg tgagttcttt agtaactaca gcatgatctt tacagctgtg atggtggcat
     3301 ctattcctat ggtagttatg tacttgttaa tgcagaggtc atttattgct ggccttacag
     3361 caggagcggt taaaggttaa tgagcagcca cacgccagtt gttagcaatc ctccaagact
     3421 tcagggtcag gtgcgcctat tacttgaaca catagaaagc caagggcttc aagggggaga
     3481 tcgtctggaa ccagagcgcg aactagcaaa aatccttgga attagccggc cttcacttcg
     3541 agaggcgatt cagattctgc aagttcaggg gcgtcttagg gttaaacatg gaatcggaat
     3601 ttttgttctt gaccaagcag acggggagaa gttaagagaa tctctaagag cagcgcaaca
     3661 tcgcattgaa gagttgtttc aaatgcgcga aattttggaa gccccagctg ttgagtgggc
     3721 tgctgagaga agaagcgaag agcaactggc tgagatgaaa ttagctgcaa aagcgcttaa
     3781 tgaagctatt tcaaaaaaac ctgttgactt tgagaattta aggcgtctag atatggaatt
     3841 tcatttaaca attgttcgaa gcgcgcagaa tcaattctta aatcaaacac ttggaacttt
     3901 gcaagaaatt atgtatcact ctatggataa cacactaaaa cttcctggtc gagtggatag
     3961 ctcagagcat gagcatggaa tgatcttgga tgcaattgaa aagcgagatt caattaccgc
     4021 ccgattgatg attattcagc acattcataa tgcccgcgat gcctggcaga agtatcttaa
     4081 gaagcagagt gatctatgag ccaggttcta tgtgtgggtg tagcaacagt ggacaatata
     4141 gttttggtgg ataaataccc tgctgcaaat caaagagttg tagcacttga ttcaactcga
     4201 gcagtagggg gcccagcaac aactgctgcg gtaacactag caaggctagg tattgaggtc
     4261 tcgctatctt gtgtaatcgg agatgacgaa gagggaagat ttatctttga aactctaaaa
     4321 aatgaaggta tagatacaag tcatgttgta gtcgatgcaa atgttagaac tgctagagga
     4381 acgatagttg tttcaaagag cgagcagagc cgagcgataa tggtgatgcc tcatgatcaa
     4441 atacctaaaa agccccttga taccagcaaa tatcaatgga ttcacgttga tcaagctgga
     4501 atgagggcgc taaagacttg gggaattagc agaggtggat ctgccaagct ttccattgat
     4561 attggctatg ccactgaagg attaaatcct ggtgattatg atctttatgc gccatctgaa
     4621 aatataacaa cagatacaac aagcgcggtt aaagataaga acattgttgt tatttcacaa
     4681 ggagaaaaag gaagtcttta ctgcgatggt cttagctctg gaagtgttcc agcgatcaat
     4741 acagaaattt tgagcacact tggtgctggt gatgtttttc atggcgcact tgttgctgcc
     4801 caagtttgga ataaagaaat tagcgaagca gtattaatag caaatactgt ggcaggtctt
     4861 tcatgtagag gccttgatgg acaaagtgga attcctagaa agaatgagct ggatgcctat
     4921 ttggctaagg cgaagatctg atggggataa cgggtaatcg agatatggaa aggaagtaat
     4981 gatggccaaa gtgcctgact ttctcctcca ccatgatggc gattcagttg ctgttgcaaa
     5041 tgatgatttg caacctggca cactgcaagg gctatcagtg aaggagcaga ctgcacatac
     5101 tgcagtatta aatgagccaa ttcccttggg gcataagttc gcacttcgcg atcatgccga
     5161 gggcgaggcg atagttaaat atggaattaa agtagggatt gcatctaagg caatcaagaa
     5221 gggcgattac gttcacacac acaacatgag gagttatcga tgggaagcaa gccgcgctta
     5281 actggatacc gtcgccctga tgggtcgatg ggtatccgta atcacgtcat catcttgcca
     5341 gttgatgact tatcaaacgc tgctgcagaa gcggtagcaa aagtagtgcc aggcactctt
     5401 gccttgcctc atagctatgg acgcctgcaa tttggcgaag atcttgaact tactttccgc
     5461 acacttatcg gcactggcct aaatggcaat gtcgcagcgg tagtagttat tggaattgaa
     5521 cctaattgga cccagagagt tgccaatggc atcgctaaat ctggaaaacc ggttgcttca
     5581 ttttctattg aaggcaaagg cgatcttcaa acaattgctg atgcagcaag agttgctcaa
     5641 gtcttcttgc aagatgcatc agagattgtc cgcgagagcg caagtgaagg tgatctaatc
     5701 ctttcaatta aatgcggaga gtcagatacc acatcaggtc ttggctcatg tccaaccaca
     5761 tcagaggcag tagatcgttg ggttgcagca ggagggacag ttttctttgg tgaaacttca
     5821 gagctaactg gcggcgaaca tctaattgca gatcgctgca ttgatgacgc ttgtcgaaat
     5881 ctcttccaaa ctacctatga caactacatc aaagtaattg aatcaaccgg tgcaaacttg
     5941 cttggttcac aaccaacgca aggaaatatc gcaggtggtc taacaactat tgaagaaaag
     6001 gctctaggaa atatcgctaa gaccggatct gttccagttg ttggagtttt agcaccagct
     6061 gaggcgccac caagaaataa gccaggtctt tatttcatgg atacttcatc agctgctgct
     6121 gaatgtgtaa ctctgatggc ggcagcaggg gctgtgattc acctatttcc aaccggacaa
     6181 ggaaatgtga tcggtaatcc aattgagcca gttttgaagt taactgccaa taagaagacc
     6241 gctgcatcaa tgccagaaca catcgatctt gatgtctctg gtctattgcg tcaggaatac
     6301 aacttgacca aagctggcga catgatgatg gatgtaattg agcgaactgt taatggaaga
     6361 ctctgcgctg cagaggctct tggacatcgc gaattcacct tcaccaagct ctatatcagc
     6421 gcctagaaat aaccctcgat tacccgaaga aaaaggactg gttcgtgata ttaattagtg
     6481 aagatgtttg gagcgctgac tttgaagcgc tggctaactc ttttccatta attcgcgaac
     6541 cagaactttg gagcaacgca gaagagttaa aggcaaagct tgaatctgca acagcattag
     6601 tggttagaaa tcgaactcaa gtaagtagag acttgattgc atctgctaag aatttaaaaa
     6661 ttattgcaag ggccggcgta ggtctagata atattgatgt aaaagctgcc gatgaatttg
     6721 gcgtggtggt gagcgctgct cttggcataa atgccaccgc tgtggctgaa gagacgctag
     6781 ctatggcgct tgctctatct agaaagttaa ttgaattaga ttcaagcaca agagctgggc
     6841 aatggaatag aaaagcagga gttgagatct caggtaagac ttggggacta ctaggatttg
     6901 gtgcaactgc tagagcaact gcagaacttc taagaggttt taaagtaaaa gtcttggcct
     6961 atgatccatt tgcaaaacca gagcagaatt atctagattc cataaattgc cagatgagct
     7021 cacttgatga tgtgatttca aactcggact tcatatccgt ccatctgcca gctactagtg
     7081 agactaaaga gatcattgat agtaagaagc tttcggttat gaaaaagagc gctctactaa
     7141 ttagcgtggg gcgcggtgag gtaattaacg aagctgacct agagcaagct ctaagagatg
     7201 gcgttattgc aggagctggc cttgatgtta gagctcaaga acctccaaag gataagcgct
     7261 tcacagatct tgataacgtt gttttagctc cgcatatcgc tggtattacc catgagagtc
     7321 aggctgcaat caatcgagta ttagtaagtg agataagagc agccctaagt ggccaggtgc
     7381 aaagttatgc cgttggcgca gtaaagcagg caaagtaatg gcaatctcta tagctagagc
     7441 ccttgctcat gccacatcac ttcttattaa agctggagtc aatgaagtta attcagaaaa
     7501 aactgctcgc gccatagtca cctctgatgt ttggggtaat cccagccatg gacttatgcg
     7561 acttcctttc tacttgcaga gattgagtca gggcggggtt aatccaaagg ctgaattaaa
     7621 agtaatttca gaatttggct ccacaatttc acttgatggt caagatggat taggacactg
     7681 gcaacttctt gatggcgcgc agattggcgt aactaaagca aagcagagtg gaatctcact
     7741 tgtttcaatt gctaactcaa gtcactgcgg tgcccttggg gtttatctct atccggcact
     7801 tgatgcagaa atgatttcaa tgatttttac caatggccca gcggtgatgc cagcagttgg
     7861 tggaaattca ccaattcttt ccaccagccc tatcgcttca ggaatcccat caaatcctcc
     7921 aatgattatt gatctttcaa catctgctgt tgcaagagga aaaatagcta gcgctgctaa
     7981 agctggagga tcgattccag cgggctgggc agttaatgaa aaaggtgaag caattactga
     8041 tgccaagcaa gctcttatgg gaatgcttgc tccacttggc ggagcaaagg gatttgcact
     8101 tggtttgatg gttgagtcac tctcagctgg tcttagcgga ggatctttat ctagagctat
     8161 tccagatatg tttaatccag atgatgataa aaaagcgcag ggaatttctc acacggtaat
     8221 aactattaat cctgcaagta ttggtaaaga ttctaaagaa gggcttgatg aacttgccgc
     8281 aagtattacc gcaagtgggg gaagagttcc aggaagcaag cgagtaagtc ctaataattt
     8341 aggaagtggt gaaataaccc ttgctgatgc ggtaattaac gatcttaact cttggagcca
     8401 aaagcttggc ttagagaaca tctcatgaag ttaacatcta gcaatctaaa gcatttttca
     8461 gtcatctcta tttttgttgc tctggcatat gggatcagcc aattgcaggg cgcaatttct
     8521 ccgctattta tctcactgct ctttggatta gctttagtta acagtggtct ctggggcgat
     8581 ggcggcagag aggctgcaaa ctttgcagca aagagatgta tgcgcattgg ggtagtactt
     8641 cttggctttc agatctcaat tgataagttc attgaagttg gcccaaaggg gctcttggca
     8701 gtaattatta ttgttgccgt tgtttttcta ggtcttagat atgtagctat gaaaagtggc
     8761 tccagtgaat ctttctcaac tcttattgca ggcggctttg caatctgcgg tgctactgca
     8821 atagctgcga ttagttcaac tcgaaagagt gaagagcgag atgtttccta tgctgtggca
     8881 cttgttgccc tctgtggcac gctctctgtt tttgtcattc caccacttgc aaatctcttc
     8941 tcgctaagtg atgcaactgc aggtgcttgg attggcgctg cagttcatga tgttggacaa
     9001 gttattgcca ccgcatcact tatgggccct gccgcacttg atagcgcagt gattgtgaag
     9061 ctaacaagag ttgttctact cattccacta attctcttgc tctcctataa aacaagtgag
     9121 aagaaatcct taaagagcgc cactccagta tttgttattg gctttgttgc ctgcgcctta
     9181 attgttaatg ccctcgcact tccagagagc gcaattaatc taggaaaaga gagttcaaag
     9241 atcttcttat ctcttggttt attaggaatg ggtctttcgg tgaagtgggc atcaattaag
     9301 gctcttgggg caaaaccact tgttttaggg ctgcttgcct gggtggtttg cgggggattt
     9361 gccttggctg tcatcacctc cgttgggctc taggcgcgaa tttccctttt ctaaccccct
     9421 ctcataaact ccccctctgc ccaaatgagg gctcgaggca aacaaaacga ggttaacgct
     9481 tggctaaaaa agacggtgca atcgaaatcg aaggcactgt agctgaagcg ctaccgaatg
     9541 cgatgtttcg cgtagagctc accaatggac ataaagtgct tgcacatatc agcggaaaga
     9601 tgcgacagaa ctacattcgt atcttgcctg aagatcgtgt gattgtagaa cttagtccat
     9661 atgacctcac cagaggtcgc atcatttatc ggtataagta agaaagggag tctgatcgtg
     9721 aaggttaaac caagcgtgaa gaagatttgc gataagtgca aagtcattcg tcgtaatggt
     9781 cgcgttatgg ttatttgcga caatctccga cataaacaac gccagggtta aacctggttt
     9841 aaaaaaaact aaatataaga acgcgtggcg cgacagtaag taattactga cctcctgact
     9901 gaaagcggga gccagagaaa attctctgga taggcgttgc ggaggacttt cagattatgg
     9961 aaagaagtta tagatggcac gtctagttgg cgtcgatctg ccgcgtgaaa aacggcttga
    10021 ggtcgcgctt caatatatct tcggcattgg ccagaccagg gcgcaagcaa ctcttgctgc
    10081 aaccggcgtc tcaccagatg ttcgagtaaa agatctacaa gagcccgatc taattaagct
    10141 tcgtgaattt attgaagcta attacaagat cgaaggtgat ctccgccgtg agattgcagg
    10201 cgatattcgc cgtaaagttg aaattcagtg ctaccaagga attcgccacc gtaaggggct
    10261 tccagttcgt ggtcagcgca cccataccaa cgcacgtact cgtaagggtc cacgcgttgc
    10321 catcgctggt aagaagaagg agaccaagta atggccgcgc caaaagctaa acctgcagct
    10381 actgctgctg cccctaaggc aaaagtaaag gcacgtaaga aagagaagaa gaacgttgcc
    10441 cttggaaatg cctatataaa gagcactttt aacaacacca tcgtgactat cactgatcca
    10501 tctggagcag ttcttgcttg ggcatcatct ggccaagttg gatttaaagg ttcacgtaaa
    10561 tcaactcctt tcgctgcgca gctagctgct gaatctgctg ctaagaaagc gcaagagcat
    10621 ggagttaaga aagttgatgt ctacgtcaag ggcccaggct caggacgcga gactgcaatt
    10681 cgttcactac aagcagctgg actagaagtt ggtgccatct ctgatgtcac acctcttgct
    10741 cacaacggct gccgtccacg taaaccacgt cgagtataag gaagggagaa gagaacatgg
    10801 cccgttatgc aggagcagat tgcaagagat gccgccgcga gaaagtgaaa cttttcctca
    10861 aaggctcgaa gtgcgatgga cctaagtgtc caattgagtc acgcccttat ccaccaggac
    10921 aacatggtcg tgccagagcg aaggaatctg agtacttact ccagatgcgt gagaagcaga
    10981 aatgcgcacg tatctacggt gtattggaaa agcaattccg taactattac accgaggcaa
    11041 gccgtttagc tggcaagact ggtgagaacc ttcttgttct tctagagact cgtttagata
    11101 acgtgatcta tcgcgctggc tttgcaaaga gccgcgatat ggcccgccag attgttcgtc
    11161 atggacacat tctggttaat ggtcgcaaag ttgatattcc atctttccat gtcagccaat
    11221 tcgacatcat tgatgtgaga acaaagtcgc aagacctaac tccattcatt gttgcatctg
    11281 ctgaatttgg tgagaagtca gtaccggctt ggcttgaagt tgtgccatct gcgatgagaa
    11341 tcatcgttca tggtcaacca actcgcgcca ttatcgatac tcaagttcag gaacaactta
    11401 tcgttgagct ttactccaag taattaataa ggcagagcga tctgcctcaa ttaatcatct
    11461 ggctagcaag gatttaaaaa cttcataaaa gtaggtacta agtgagagat caaatagtgg
    11521 atctctcaag ataatggagg aaagaagtgc taattgcaca gcgccccgta ctatccgagg
    11581 aagtagtaaa cgagagccgt tcccgtttca taattgaacc gttggagcct ggctttggat
    11641 acaccctagg aaattcactg cgtcgaaccc tgctctcatc tatcccagga gcggctgtta
    11701 caagcatccg tgttgctgga gccttgcatg agttcaccac tttagaaggt gttaaagagg
    11761 atctgacaga tatcgtttta aatatcaaga acttagttct ttcatctgaa aatgatgagc
    11821 catctgtggc ctatattcgc aagaatggct caggagttat tactggcgca gatgttgctg
    11881 ttccaactgg agtagtagtt cataacccag atcttcatat cgctacttta aataccaaag
    11941 ctaatgtgga gattgagcta actattgagc gcggccgtgg ctatgtcaca gcagtgcaga
    12001 acaagttagt aggagcagag attggtcgta ttccagttga ctccatctac tccccagttc
    12061 ttcgcgtgac ctataaggtt gaagcaactc gtgttgagca gagaacagac tttgatcgct
    12121 taattgttga tgttgagaca aagcgttcta tgagcccaag agatgcgctc gcttctgctg
    12181 gaaagacttt agttgagctc ttcggtcttg ctcatgagct taattacgca gctgaaggaa
    12241 ttgatcttgg accatctatt caagatgccg cacttgctgc agatcttgct cttccaatcg
    12301 aagatttaga tctaacagtt cgttcctata actgcctaaa gcgcgaagga atccacaccg
    12361 ttggagagct tctctcacgt agtgaggctg atctattaga tatccgtaac tttggttcta
    12421 aatcaattga tgaagttaag gcaaagcttg tctcaatggg catgcaactt aaggattcac
    12481 cagttggctt tgatccgaca caacatcaga actatggcgt cgattactcc gacgaccagg
    12541 cttaaggaga gagctaatgc cacgtccatc taaaggtccg cgtcttggaa gcggtccatc
    12601 ccatgaaaga ttgctcctaa gaactcttgc agagcaactc tttgaaaatg gcaaagttcg
    12661 caccaccgaa gctaaagctc gtcgtctgcg tccacttgct gaaaagttaa tcaccactgc
    12721 caagaagggt gatctagctg ctcgtcgcca agtaatggcc gctatctcaa ataagggcgt
    12781 agttcatact ctctttactg agattgcgcc acgctttgaa tccagaaatg gcggctacac
    12841 aagaattacc aagattggtc cacgtaaggg cgataacgct cctatggcaa tcattgaagt
    12901 aatagctgaa ggaactccgg ctaagaaagc ggtagttaat gaagcagaga aagcagctaa
    12961 gaaggcagcg ccaaagaaga aggctgccgc cgctaaagcg gagtagctct ttctaataaa
    13021 aaaagtaaaa aaagaagtgt caatggagca acccactctc aaagttgaga gtgggtttct
    13081 tcgttttagg attgattttt cctatgatgg aagcgccttt agtggttggg ctaaacagcc
    13141 agatcagaga actattcaag gtgagatgga agttgctctt agcggtttag ttagaaataa
    13201 agttgattta atcgttgctg gaagaacaga tgctggcgtt catgccagta tgcaagtcgc
    13261 tcactttgat ctgccagagc gtgataaata tggaaagcct tggaacactc aagatctgca
    13321 atatcgcctt aatagaatgc taagtgaaga gattttggtc agagcagtta gcagagctcc
    13381 agatcatttc catgctcgct tttctgctct gcgccgaact tatatttata agattgcaga
    13441 tggtcaacag catattgatc cgttaaagag atttgatatc acgccttggt atcgacacct
    13501 tgatttaagt aggttaaatg aggcaagtag gagattgctt ggagatcatg attttgcaac
    13561 attttgtaaa cctggtggct ctggaactac cttaagaagg cttgaggaat ttagctggca
    13621 gcgaatggct gattcaaccc ttcaagcaag agttactgct gatgctttct gttattccat
    13681 ggtgagaaat ctagttggag cagtagtctg tgttggagag tctcgctttg agccagagtg
    13741 gatttcatcg ctacttgcca ataaaactag agttagtgag agcatggtat ttccagcacg
    13801 aggtttaacc ctgattgcta ttgagtatcc agctgatgat ttacttgaag ctcgctctaa
    13861 agtcactgct cgcagaagag atgaagagta aatggggcag ataaatgtag ttggaattga
    13921 gtatgccctc tcagatggtc gagttttact tagcgatgtt tcatttcgag tcggcgaggg
    13981 atcaaaggtt gctctgattg gccctaatgg atctggcaag acaactcttt ttcgaatgat
    14041 tgccggagat ataactgctg atgctggtca gataattatt aatggtggcc ttggtgtgat
    14101 gcgccaattt attggctcga ttcgagatga atcaacggtg agagatttat taatttcagt
    14161 atctcccctt cgaatcaggc aagctgcaga gaaacttaaa gaggctgaaa ttgaaatggc
    14221 tgccaataat tctgagagtc agcagatggc atatgcccaa gcactagccg attggggaga
    14281 tgctggggga tatgaacaag aagtgctctg ggatattgca gttaataacg cgctagcaaa
    14341 gagctttgat cgagtatctg atcgattagt gaattcactc tctggtggag agcaaaagcg
    14401 ccttgtcctt gaagtattac ttaatggacc agaggaggta ttaatccttg atgagcctga
    14461 taactatcta gatgttccag caaagagatg gcttgaggag cagataagaa tttcaaagaa
    14521 aacaatccta tttattagcc atgatcgtga gttattggca gcaactgcta ccaaagttgt
    14581 cactcttgaa caaggtgtta gtggcaatag cgcctggatt catggtgagg gctttaatac
    14641 ttggcatgat gcaagaaaag ctcgtaatgc tcaattcgct caagtactag agcgctggca
    14701 agatgaacat aagcgtctaa aagaactagt tctaactctg caatggcaag cagcgtcatc
    14761 accagatatg gcctcaagat atcgagcaat gcaaaccagg ctcagaaagt ttgaagagat
    14821 cggcccgcca cctccgccgc ctctagaaca agatgtgaag gttaatttaa agagcgggag
    14881 aactggcgag cgcgtagtga cctgtaagga tttaacttta gagaatctaa ctgagccatt
    14941 tacctttgaa atctattatc aagatcgggt agcaattctt ggaaagaatg gttctggaaa
    15001 atctcacttt ttaagaaggc tttcaggtga tgagagcgtt agatatagtg gcgagtttaa
    15061 actcggtgca agagtaattc caggtttctt tgctcagact catgcccatc ctgaatttgt
    15121 aggaaagact ttgttagaaa tcttatggca ggaaaactct ttgcaattag gggcagctaa
    15181 atctgttctt ggtagatatg agttggccca acaagcagag caacaattca catctttatc
    15241 tggtggacag caggcacgct ttcaagttct attattagaa cttgctggcg atactttgtt
    15301 attactagat gagccaaccg ataacttaga tctggcaagt gctgaagcgc tagagcgagc
    15361 actcgatgct tatcaaggaa ctgttattgc tgtgacccat gatcgctggt ttgctaaagg
    15421 atttactaga tttttgatat ttggcgcaga gggtgcggtc tatgagagcc ctgaaccagt
    15481 ctggagccat taatcaggct cgattttgac ccctttgcat agtgcaggta atcttcgcct
    15541 tctgtctccg ctagctgtgg agcggcacta aatagaaaag taagaagaaa gagagtctgc
    15601 ccgtgcgcac cttttcaccg accccgaagg atgttgcgcg tcaatggtat gtaatcgatg
    15661 ccgctgatgt cgtccttggt cgccttgcta gccatgccgc tatcttgctt cgcggaaaac
    15721 ataaaccaac atttgctcct catatggata tgggagattt cgtaatcgtt attaatgccg
    15781 aaaaaatcgt tttaactgga tcaaagatgg ctaacaagat ggcctatcag cactcacaat
    15841 atccaggtgg attaacagag cgcagctatg aactattgat ggagaagttc ccaactcgcg
    15901 ctgttgaaaa ggcaattaag ggaatgcttc ctaagaactc tatcggcaag cagatggcct
    15961 ctaagttaaa agtttatgct ggccctaatc atcctcatgg atcacaagga ccaatcccat
    16021 atgtctttac ccagatcgat cagatgaagg ttggtaagta aaagatgagc gaaaaagaag
    16081 agaacgcaac cgcctttagc tctgcaaccc ctgctgctgc aactaaccgc gctgctgtaa
    16141 ttgcagctgg cgcaggtatt ggtcgacgca aggaagctgt tgctcgcgtt cgtctaaccc
    16201 caggaactgg tcgctggatt gttaataata agaatttaga agtttatttc ccaaataaag
    16261 ttcaccaaca ggcagtatct gagccacttc gcaccgttgg cgctgagggc aaatatgatg
    16321 ttattgctcg cattgatggt ggcggaattt caggacaagc cggagcgctt cgcttaggcg
    16381 ttgcaagagc tctaaatgag attgatactg atgcaaatcg cccagctcta aagaaggcaa
    16441 aattcctaac ccgtgatgct cgcgtgattg aacgcaagaa atatggtcta aagaaagcac
    16501 gtaagcgttc tcaatacagc aagcgttaat taacaaagag ggaaaaatgg cgctctttgg
    16561 caccgatggt gtccgaggcg ttgctggcgt tgatctaact gctgaactag cttttaacct
    16621 ctcaactgct gccgcatcag ttctggcaga tcttggtgag tttgctggcc atcgcccaac
    16681 tgcaattgtt ggccaggatt caagagcctc aggacagtat ttagaggagg caatctgtcg
    16741 cggtctttca agtgctggta ttgatgttta tcgagttggg attcttccaa cccctgctat
    16801 ctcatttctt gttaaagatc gaggagcaga tcttggtgtg atgattagcg catctcataa
    16861 tccagcaagt gataacggaa ttaagttctt tgatagaact gggtcaaagc tagctgatca
    16921 aattgaagca aagattgagg gcgtgttagg caaaacagta gtaagcaata atggcgctgg
    16981 cagagtaatt gaagattttg aggctaaaga gagttatatt aaaaatcttc taaagagtct
    17041 tgatggcaat ttaagtggga taaaagtagt tgttgattgc gccaatggag ctgcctcatt
    17101 tgttgcacct gatgtactta aaagagcagg agcagaggtt gttgccattt cagcatctcc
    17161 caatggttta aatattaatg ataactgcgg ctctactcat ctagagagct taattgcagc
    17221 tgttaaagaa gagggagcag atattggaat tgctcatgat ggcgatgctg atcgagtcct
    17281 agctattgat gataaaggat cagttattga tggtgatttc atattgggaa ttcttgctca
    17341 agatatggag ctaccaacaa agactgttgt gggaactgtt atgacaaatc ttgggcttat
    17401 taaagcttta gaggaaaaag gtattaagtt cattgcaaca gctgttggtg atcgctatgt
    17461 cttagaggag atgcttgctg gcggacatgt tctaggcgga gagcaatcag gccacatcat
    17521 catgagtaag tatgcaaata ctggcgatgg tttattaacc gcgcttcagc ttatttcaaa
    17581 acttaaaaag agtggaaaaa gtgcaagtga attagcctcc tttatgaagc gttatccgca
    17641 agttctaatc aatgtctcag gagtagcgaa agaaaaattg gcatcaaacc aagtcattgc
    17701 taaagcagta agtgatgtag agaaaaaatt gtcagataaa ggtcgagttt tgctccgcgc
    17761 ctctggaact gaggctctaa ttagagtgat gattgaggcc caagatctat ctttggctca
    17821 agaattagcc tcagaactgg caacgctagt aaaatcagag ctctcacttt aagggagcca
    17881 atttaaatgt gcggaatcat cggttatacc ggagcctcac ctgccctttc aatggtgatt
    17941 gattcgcttc gaaagttgga atatcggggc tatgactcag ctggaattgc tcttactggc
    18001 aaagcgggtg agaagttaat tgtggcaaag cgcgccggga agttaagcaa tttggagagc
    18061 gcgctaactt cagatcatgc tgattctcta agtggaattg gccacactcg ctgggcaact
    18121 catggtggac caactgatga aaatgcccat ccgcatatct caagtgatgg aaaagtagct
    18181 gttattcata atggaattat tgaaaattac ttagaactaa aagttgagct agaaaagcgt
    18241 ggccataagt tcgcatctca aaccgatact gaatcagttg ctcatctctt aagtgatgaa
    18301 tttaatttga gcaaagattt ggcagatgcg atgcgcaagg tttgcaagcg acttcgtggc
    18361 tcttttactc tccttgctat ctcatcagat agccctgagg tgatagttgg tgcaaggcgt
    18421 aattcaccgc tagttgtggg tcttggcaag ggtgagaact ttctagcatc tgatgtctcg
    18481 gcatttatcg ctcataccaa gagcgcttta gagcttgggc aggatcaagt agtgcaggta
    18541 agtcctgact cagttctagt aacagattta gatggcaagc aagtcagcgc taagcctttt
    18601 gagattacct gggatgcaaa agcagcagag cgcggcggat atacccactt tatgctcaag
    18661 gaaatcttcg aacagccgaa agctatatct gaaacgctac ttggaagatt tagcgatgac
    18721 tcaaaagtta tttgtagcga gctttcaagc cttaatctag atattgaccg aattttgatt
    18781 ttagcttgcg gcactgcata tcacgcagga ttaatcggaa aatatgccat agagcaatgg
    18841 tcaaaaattg ctgttgatgt tgaactagcc tctgagttta gatatcgaaa gcctttttta
    18901 actccaaaaa ctttggtaat tacgatctct caatctgggg agactatgga tactttgatg
    18961 gctgctcgcc atgccaaaga tcttggagcg gcaactcttt ctatctgtaa cacgatgggt
    19021 tcatcaattg ccagagaatc tgatgcagtc ttttataccc acgccggccc tgagattgca
    19081 gtggcatcaa ctaaggcgct tctaacacag attgtggcgc ttaaagttat cgctctttat
    19141 ttggctcaga acaataagag tttaagtggc gcgcagattg cagcaatcat ttctgagtta
    19201 cttgccctgc caaataaagt tgaggagatt ttagaaacag ttgaaccact tcgtgagatg
    19261 accagaggct ttaaagatgc aacttcagtt cttttcctgg gaagaaatgt gggatatcca
    19321 gccgcgctag aaggcgctct taaactaaaa gagcttgctt atatgcatgc tgaaggtttt
    19381 gccggcggag agctgaagca tggacctatt gcactcattg aagagggaac accggtaatt
    19441 gcaattctgc ctcctgcatc atcgccgata gcagagaaaa tgcttagtaa tattcaagaa
    19501 gttagagcgc gcggggcaaa tgtgattgct attgcagatt cttcgatttc gctagatgtt
    19561 aaaaatatta ttagaattcc aagctgtgat gaattattac aatcaatact tgcaatcgtt
    19621 cccttgcaag tttttgccta tgaaatggct gttgcaaggg gaaatgatgt tgatcagcca
    19681 agaaatctcg ctaaatcggt gacagtggaa tgagtggtcc tagtagcctc tttgatgatg
    19741 ttgtagatca aagaattgag attcgtcgtc gggagatgaa tattgccctt acttggtgcg
    19801 cagtattact tcttggcttt gctttagtaa ctcttgatgt catcaataag ggcctgctct
    19861 atagatttga tcaatttgta gcagatctaa gtcgccctaa gctctctgga tttactaact
    19921 ttttagttct tcgaatcgat gacttaggtc ttagatggat taccgcgctt atcttgctga
    19981 ttaccgcagg actgatcagt agaagattta aatcttttag acctttaaat ttagcttttc
    20041 tatctttagt atttttaaat atctttgtag gcgcttctaa gattttcttt ggaagatgta
    20101 aagcaaaaga agattttgac ctctgtatgt ttagcgatgg tatggcatat cccagtggcc
    20161 atatttcaaa tgcccttgta acttgggggc tctttgccta cataatcttt cgttataccc
    20221 atagagctcc atttgaaggt ttaaagctct attggttggt agctgttatc actattgcag
    20281 taggagctgt ttcgctaatc cgtaataccc actggtttac cgatcttctt ggtggaatgt
    20341 ttttaggtgg agctatattg gttttagtag ttgcaattga tcgctttatt ccctcgaccc
    20401 gtcagccctc ataggtatat aggaaaaagt agcgattaac tatggagaat cgggcagaag
    20461 tacttatcga tcttggagcc attagagata acatcaaata cttaatggct aaaagtggta
    20521 aaccagctct ggctgtagtg aaagcagatg gttatggcca cggccttctt ccagtagcaa
    20581 agagcgcgct tagcgctggc gctagctggc ttggggttgc actacttgag gaggcaagag
    20641 ctttaaggca aggcggaata agcgcgccaa ttattgcttg gttaaccccg atcacagatg
    20701 atttcaaatc tgccataaat gaagatatag atattgcaat cgcatcactt gctcaccttg
    20761 aggcaattac tagcgcttcc aaatctttag gaaagtgcgc cagggttcat ctagaagttg
    20821 atactggaat gtctcgcggc ggggcgcttg gtgagtggag tgacttagtg cgcagcgcca
    20881 aggcttatca agactctggg cttattgaag taattggtat ttggtcgcat tttgctagag
    20941 ctgatgaagc tggacatgaa tttaacaaaa agcaattaga aaactttaag agcgcagtag
    21001 cgcaagcaaa gagcgttggt attactccgc aggtaattca cttgagtaac tcagcagctg
    21061 cgattaatga tgttgattca cattttgatc tgcttcgttt aggtattgca atctatggac
    21121 tatcgccaga tttttcacat atgggaagtt ccgaaaaact aaatttaaaa cctgcaatga
    21181 gtatccgcgc cagaattcat ttagtcaaag atgtcccagc aggctctcag gttggttatg
    21241 gaggaagtgc tataacaaaa gttgatacca aacttggagt tattgcaatg ggttattcgg
    21301 atggaattcc aagaaatgca aatagtgagg cgggagtact ggtaggtaat aaacgagcac
    21361 caattattgg tcgagtatca atggatcaat ttgtggttga tttgggcaga gatagctcgg
    21421 caaaggctgg agattgggca tacattattg gaagtaatcc tttggatagt tatacttcgg
    21481 caagttctta caccgccgat tcgtgggcta gcgctagcgc aacgatcaat tatgagatcg
    21541 tgacgcgaat agcagctaga gtaaagcgaa tctatcaagg ctaaaggagg gcgcatgaca
    21601 gaaggcgttg tgctctctgt taatgatctc tcagtaaatt ttggcggcct taaagctcta
    21661 aacggcgtca actttgaatt aagaaaaggc gaagtcttag gacttattgg ccctaatggt
    21721 gccggaaaaa ctaccgtctt taatgcgctc tgcgggattg tcactccaga ttcgggctct
    21781 ctttctttaa atggcaaaga aattaaatgg ccaaagcctc ataaattagc gaagttaaaa
    21841 attgctagaa ctttgcaagg tgttggactc tttccagatt taactgtcta tgacaatgtg
    21901 atgattggcg ccaataaatt ttctgaaaca ggtttacttc gcgcagcctt tggaacaaac
    21961 cataaagatg aaaagcgctt aaaggagcgg gcgcaagcag ctcttgagcg agtttatgtt
    22021 ggcgcagttg cagatagaag agcagacact cttgcctatc ctgatactaa gcgagtttct
    22081 attgctagag cattggtagc agagccagaa atattattac tagatgagcc tgctggagga
    22141 ttaggcccgc aagatattga atggctcaat agccttatta ataatcttaa gtctcgcacc
    22201 tcaataatta tcgttgagca tcacatggat gttgttatgt cagtctgtga tcgaatctat
    22261 gttttgaact ttggcgaaat aatcgcatct ggaactccag cagaggttag agataatgaa
    22321 gcagttatcc aggcatatct tggaaccaaa tcaaaggcca agtcatgagc ggattagtag
    22381 ttaatagact tagcgtctcc catggggcaa ttgaggcgct tcgtgcagtt tcttttagcg
    22441 ttgaggttgg tcaacttgca gccattattg gttcaaatgg cgcaggaaag acaacactgc
    22501 ttcgcgccct ctctggtcta aatactcaaa cttcaggaag tgcgcagtgg aatgagcgag
    22561 aaatccttgg ggcaaggcct gatgcgctag taagaagagg tattagccat gtctctgagg
    22621 gtaaatctgt aattgcagag ctaagcgtta aagagaattt gatcttgggt ggaatctgga
    22681 gaaacaaaaa gagtgatacc agcgcagcta ttgcagaggt agttgagctc tttccagttt
    22741 tgggacagag aatgagccag agcgccgact ctctctcagg cggcgagcga cagatgctcg
    22801 ctattggtag agcgctagtt tcaagaccaa aattattact actggatgaa ccatcacttg
    22861 gacttgcacc acttgttatt gaacagattt ttgcaactat tgatcaactt cgaagaaaac
    22921 tcgggctaac tgttgttcta gttgagcaga atgcaatgag cgcgttagag attgctgata
    22981 tcggaatcat tattaatctt ggcgaagttg ttgcctccgg caaagccaat gagctaattg
    23041 atgatcccgc cctacgagct gcttacttgg gatactgatg caaaaagtta ttgaaactct
    23101 attaatcggg ctatcaacgg gatctattta tgcccttatg tcattagcac ttgtcttagt
    23161 ctggagatca acgcgggttg ttaactttgc gcaagcagga caggcgatgc tctccaccta
    23221 tattggcttt gagattgcat ctagatctga gattttctgg ttaggacttg tctttgcaat
    23281 tgcagcggga gcactagttg gtgcaggcgt tgattacttc tttatgcgcg tgctcttcaa
    23341 acatgcaact tctggacctg cagcagttgt tgcacctgtc attgccactc taggtttact
    23401 tggccttatc agaagcattg tgggattaac ttggggcgga gaatttaaat ctattgaagc
    23461 gccagcctca acagatggct ttactgttgg agatggcaca attccatttt caaatctcga
    23521 tgttttaatt attgctacag tttcaataat tctcattgct cttgcctata tcttccagcg
    23581 caccaacttg ggtcttgccc ttagggcctc ggcttatgcc cctgaaatcg ctcaattagc
    23641 tggcattaga acagcacgga ctagaactat tggatggtca attgcgggag ccgtgggcgg
    23701 agttgctgga atgttagtaa cttcaaataa cttcctctct ccttattcgt tggatctctt
    23761 acttgtcttt ggatttattg cagctgttat tggcggtctt gaaagtatgg tcggatctgt
    23821 tatcggagca cttgtgcttg gaattggcct tacatttatt ttgaactacg ttggaacttc
    23881 tctggtcttt attggtggat ttagcgtgtt gattcttgtt ctgcttatta agccagatgg
    23941 aatcatggca aagggaaagg cccgccgtgc gtaagaaaat agctatcttt gttctcattg
    24001 gttgggtatt actaatcatt ggtgatcgcg ttgatgagct tcgcgtctat caaggagctc
    24061 agatggcgat gtataccatc gcacttgctt caattgtttt gttaactggc tattctggtc
    24121 aagtttcact aggtaatggt gcgatgatgg ctgttggtgg ttatggcgca gctcttacgc
    24181 taaataattt aggcactcca atttggtttg cattcttaat ggcaattgtt acggcctcgc
    24241 tatttggtgc acttcttgga gcagcagcag caagagtttc tgggccttac ttagcaggaa
    24301 caactcttgc cctagctgtt ggtcttccag cgattgcaaa tcaatttgcc ttccttgggg
    24361 gagagccagg gcttttattt gacgttggct tcccaccaga gagatttggc gatgaattca
    24421 cgcaatataa atggttcttc tggattgcaa gctttattgc tctgattgcc ttatttatta
    24481 cttcaaatat tctctcatcg cgctttggtc gcacctggcg cgcaattcgc ggaaattctt
    24541 tagcagcaga gctctgcgga gttcattcag ctcgtatgaa ggttctagcc ttcacagtta
    24601 gctctggcct cgcaggtctt gccggagcag ttctggcaat gactataagc cttgttgccc
    24661 ctggggcata tggcctggcc ctttcctttg ccctggtgac cggggcagtt cttgctggaa
    24721 tctcaagcct tcctggggta gtcctgggag ccttcattct cgtggtcatt cctgagatat
    24781 ctgtggccct tgccgacagg ctgggtgacg cagataaact aaacaactat ctaccaggtc
    24841 taattgtgag cgtattgctg attctctcag tgatattcac cccaaatgga ccgggcgaga
    24901 acttgcgtaa gcatcgtcat aagaagcctg cgcattaact gattaccgct aggtaaccag
    24961 tagattaata gcacctagcc aaaccctcga tggaaggaaa acaatgatca gaaagactcg
    25021 tataaagcga acctggctcg ctgctgcggt gattgcctct ggctctctcc tagcaacagc
    25081 gcttccagca aacgcagaga cgggcgttag cgcaagtgag atcaaacttg gtatcactct
    25141 gcctctaact ggagcagcat caccaggcta taacaagatc ggtaatgcaa ttaatgctta
    25201 cttcaagtat gtcaatgaca atggcggcgt ctatggacgt aaagtcactc tcattcagaa
    25261 aaatgatgag tactcaccac agttgtcgat tgctaagaca aacgaattaa ttttgcgaga
    25321 taaagttttc gctctagttg gcccacttgg aactgcaaac ttcaccgcag tccacaaatc
    25381 agttggcctt gctaagcgcg gagtcccaag ccttttccta aacactggct tcagcggatt
    25441 ttcaaataag aagagctatc catcttcttt catgattctt ccttcctacg ctatggaagc
    25501 aaagatcatc gcccaattcc tcaaagagaa ccatgctgga aagaaactcg gcattgttta
    25561 tcaaaacgat gaatttggta ttgatggtct aggggctttc aaggcagctg gaactaagtt
    25621 tgatgtttca attccttaca cctcaggcac ccagagcgcc gcaaccgcgc aaacctgggt
    25681 ctcccagctt gcagcaggtg gagctcaagt agtggtcttc tttggcgtaa cttcagcaac
    25741 tgctccactt cttgctgtag ctgctgctgc taagtacgca ccacagtgga ttcttggatc
    25801 agttggttca gatccattaa cacttcgcgc acttggcgtc ccagtagcgg tgctaaatgg
    25861 cgcacagact ccagctggta atccatcacc aaatgacact agtgatgagt atgtaaagca
    25921 gttccaagag atcaatacca agtacaacac tggtgttgcc tttgatgact atgtattgca
    25981 gggcatgaat atcggcctaa tgaccgttca agctctaaga gctgcaggaa agaacttaac
    26041 ccgcaagggt ctaattcgtg cgatggagac aaagggctca acctttgcat ctgttgctta
    26101 ctctcctctt ggtttctcaa gaacgagcaa tgttggccat accggctact acatggcagt
    26161 aatggatgcc aatggagata gaaagccatt cggtggaaaa gtaacgcttt acaccactga
    26221 cagtggctcc ggtccagttg tggtctctac cttcaaacgt ccggcaatgc cagcgaaagg
    26281 attaccaagt aactcatgag aacctttaaa ttcaagcgtt ccttaacgct cgtagcagtt
    26341 tcatcactgc tctcaattac cgttgtatca attccaacgg cagcgcaggc aaaccctgtt
    26401 tgtactacag caagcggtgt aacaacttgc cttggtgcaa catcagatac tgcgccatac
    26461 atgctgatga ctccagcaaa cttcaatgga accgtttatc tatattcaca tggatatcgt
    26521 ccaaacgttc cagtgcctgc cggaattcct ggatatggtg gctacacagt tactaatact
    26581 ccagagccag cacctggtgg aaatgcccaa attattggga cacttcttgc taagggatat
    26641 gccgttatgg gatctggctt tgcacgtcaa ggctggaatg cagattcagc aattaagacc
    26701 aatgttgagc taattggcgt tttcaagaag caatatccaa agacaacaaa ggttgttgct
    26761 tggggtaatt cacttggagg ctttattact caggctcttg ctgaaaagta tccagatcta
    26821 attagcgcag cagcgcctct ttgtatggca agtgatctaa cgccacttat gacaatggct
    26881 ggagatgcac tttggggaat caagactttc tttgatccaa ccatcaaagg tggaggctat
    26941 agcgcaggaa cagctggtta tgccgaagca atgggagatc tagtaaaggt ctttaccgtt
    27001 atcggtgcaa tgcaagctgc aattgctgca aatccatctg cgccagcgtg gccagcgaca
    27061 tcaaaggttc cagatgcact caaagctgtt ccatcaagaa gtgcactagt tcttatcgct
    27121 ttgatgtctg gtgttccaat gcaatcggca cactttgact caacttcagc gccagcaggc
    27181 cttccagcct caagcgctct ctcattccag ttagctatca atccagctct tgctgctcta
    27241 gaaaaccttg caaatgctgc agctcttgca gtgcttgcaa cacatgatgt tgagcaacaa
    27301 gcaggcgggg cagtatttga taacactgca actgattact cagcgcgtgt ttctgctgaa
    27361 caatttatct ggagctcagc tctatctggc aatgctggag taggcgctct gcttggatat
    27421 cttgcagcgg taccaagagt taagggaaat cctgcagcta ttgccaagat ggctacgcta
    27481 actacacata gtggcaaagt tgagatccca acaatcctct tcaccggaac agctgatcca
    27541 attacaacag ctggaaatca gcaatcggtg attgataaat atgctgcata ttctgcagag
    27601 aagtgggcag cagcaaagaa ggaaggaact cgtaagaaac cagctaataa tcaattggtt
    27661 ctatggaact tcccaccaga gaagtacacc aagttcaccg ctaccggttc accagataca
    27721 acggttgccg cagcgacagg aactaaccac tgtaacttca ccgtttctca gtacttagca
    27781 attgcagatc tacttgcata tgctgctgat aacggtaaga acatgtcagg tggcgcgctc
    27841 tataccaaga tccgtaaggc aggaaatatg acttacgatc gtggctatag cgctcctaaa
    27901 ctgaagtatt acggagaata atcgtccgta atccatcgag aaggtagtaa agagagggtc
    27961 tgggaaacca ggccctctct tacttttata ggtagatgtt catcagatat tcaggtaaaa
    28021 agctttggtt aactagcagc tacgccttac tgttgtcgcc atggcaagcc ttgcagagcg
    28081 aagaagaatt gccagattag ttcatcgctt tggttttgga ccgcgtcctg gcgagttctc
    28141 aaatctcttg gctaatggtt ttgatcaagc ggcagagaaa tacttaaacg ctcctagtca
    28201 agatgctttt gccgacaccc aagatgaacc gatggttaga gatcaaggaa agcgtccagc
    28261 accaaatagc cccgatgttg ttgcttatgc aaccgaaaaa agagcacagt taacttcact
    28321 ttctttctgg tggctagatc gtatggtttt atcagagcat tcattacgcg agcgaatgac
    28381 atggttctgg catggacatt gggccacctc ttatcaaaaa gtagatgatg ctctgccgat
    28441 gtatctgcag aatcaaactt tgcgaagaaa tgccttagga aactttgctc agatgtctag
    28501 agagatggtt aatgatgctg ccttaatctt ctggctagat ggacagtcaa atactgtaaa
    28561 agctccaaat gaaaatctat caagagagtt aatggaactc tttactcttg gggttaatcg
    28621 ctatagcgaa gatgatgtga aagaaactgc caaggcttta actggatata aagttgataa
    28681 atctagtgga aaagtaatct tctatcctaa gcaacatttt gccggttcta tcagggtttt
    28741 aggaacgcaa ggctcatttg atgctagctc tttaagtgat tttctagttg ctagaagtga
    28801 ttgcgcgcta tttattactg agcgaatttg gtatcgcttt atttcaagta tgaatccact
    28861 agctgatacc acccttactt caagctttgc ctcaagagat attgcagctt tagtaaaggc
    28921 aattggtaga cactctgcgc tagataatcc cgataattca atggttaaaa gtcctataga
    28981 ttggtttgtc tctgccgcta gagctttatc aattacgcca tcgaaattct ctaatcccaa
    29041 taccattcgc aactatctag atttactggg gcagcgtcca ttcttcccac caaatgttgg
    29101 gggatggccg gctgatcaag cttggctttc aacttcatct gcgcaatatc gaattcaatt
    29161 tgctactcaa ttagtcaagg aggcagatct aacgccaatt tcatcccttg ctccgcaagc
    29221 aagaattgat ggccttgctg attggctcgg agttgtggag tggagttcta gaacaaagat
    29281 ggcgctaaat ggcgcaatta aagatccggc gcgcttggct ttgctagcgc tctgttcacc
    29341 tgaatatgtt gtgagcgcat aggagcacgc catggacaca ataactcgta gacaatttct
    29401 aaaggcaaca ggtgctggag caataggcgc tgctgctatg agtctttcaa tcgaggagat
    29461 tgcaaatgca gccattacaa ggccgctgcc gcttggaact ccaatacttg ttgtggtcac
    29521 gctctatggc ggcaatgatg ggttaaatac tgttgttcca gttaatgatc cgatttatca
    29581 atcacttcgc ccaggtattg catataaacc agaggatgtt ctcccacttg gagagaacct
    29641 atatctcaat ggatcaatga gcgggatgca ttcactatgg aataagaatc aagtagcgat
    29701 tgtgcgcggc gttggctatc caaactctga tcgctctcac ttttcatcta tggcaatctg
    29761 gcaatcggca atcattggag ctgctaaaac cggatggcta ggaagatggg ttgagagtca
    29821 accagaggat ccatttcttg caattagcct tggatcaata cttccgcccc ttcttgctgg
    29881 aagtaagaga tcaggttcag ttcttccact tggcggctta aaagttccaa acggatcact
    29941 tgctagcgat tgcataaagc ttgctattga gagtcgcagc gataatttgc ttcaagcaat
    30001 ggctgcaaaa actatgcgaa atctctttac cgtctcctca gatatcaccc ctattcttaa
    30061 atcacctgcc ccagcggcgc cagatctacc aacagcgata ggcgggaatg ctggagggga
    30121 tacaaattta agtcagcaat tagatattgt ggcaaagctt gtatctgctg gtgctccaac
    30181 tagagtctgg tcagtttcac tcggtggttt tgatactcac gcagatgaaa agggatctca
    30241 agcaattctt cttggagttg taagccaagg aatttctaaa tttatgtcgc agatgagagc
    30301 tacaaataga gcaaaagatg ttgtggttct ggtctatagc gaatttggta gaagagttcg
    30361 cggtaatgca tcagatggaa cagatcatgg cacctcaggc ccagtctttc ttctaggcga
    30421 gcgggtcaaa ggtggtttct atggcgagca accatcacta aataacttga aaaatgatga
    30481 cttagctgtc actactgatt ttagagatat ctacgccagc gttttagaga aagttctttc
    30541 aacccctgcc gagaaaatac ttggcaattg gaagggtcgc actccgctct ttaactagtc
    30601 ggtaatatga ctaggtgtta attcaatcgc cagagcagat gcgagatttt ggtatctcgc
    30661 tatctaagaa atttagagct ggtgatgtaa ttgttcttaa aggcgatctt ggggcaggaa
    30721 agacagctct agcatcagga attggtatgg ggcttgggat agagggagtt agctcaccta
    30781 ccttcattat ttcaagggtt cataaagcaa aggtgccctt aattcatatc gatgcctatc
    30841 gcctcttatt aagtggcgct aaaggttttg aatttgatga tttagatatc gcaacttcaa
    30901 aagatcacgc aataacagtt attgagtggg gcgaaggcct agttaatcgg ctaagtgatg
    30961 agtatctagt aattgatatt gcttttggca cagatgaaaa tgaaagatta ctaagcgcta
    31021 caagcattgg gccaagatgg gctggattta gcctgtgaac tacctagtaa ttgatactgc
    31081 aacagatcgc agctcactag cgctttttag taataacgaa ttgatctatt caggttttca
    31141 tgatggggca accgagcatg cagaggcgct acccaaactt cttaaagagt gtttaagtgt
    31201 tgagagccga gttgatcaag tagttgttgg catggggcca gggccatata ccggacttag
    31261 agttggaatt atctttgcta tgagctttgc aaacgctagg cagattcctt ggattggggt
    31321 ctgctcactt gatggaatag atattgactc agattcatat cttgtagcaa ctgatgctag
    31381 acgaaaagaa atttacttcg ctcgttatca agatggtatt cgaacccagg gaccatcagt
    31441 ttctgcgcca agtgagatta aagatttaga tgtaaagaag tttggttttg gatttggcga
    31501 tcccctctat ccctcaatgg ctcttctcct tgctaaatct aagacacaag atgttaagca
    31561 gcctttttat ttgaggcgac ctgatgcgat tgaaagtagc gcaagatgaa ggccaccttt
    31621 cgtcagatga tttctttaga tattccactt cttgtctcaa tggagcgcga gatatatcca
    31681 gagagcccct ggagcgctaa tcaatttaag gaagagctag ctggaatgcc aaagagtaga
    31741 gagtatttag tagcgctaga tgagattgaa gttgttggtt atggcggagt tgccttactt
    31801 ggcgatgtgg cagatattca cacgctaact attaagcaga gccatagaag gcttggtatc
    31861 gcctcaagca tgctagaaaa acttgaaact tgggcaattc agcgcggcgc aaaagcgcta
    31921 atgcttgaga tgcgtgaggg caatgaagct gcgatgagcc tctatcaaaa ggctggatat
    31981 caactaattt cacggcgtga taattactat gccaagggaa ttcacgcgct aattatgcga
    32041 aaagaggtgg agcaatagat gagcgagcct ttagtccttg gtattgagag ctcctgtgat
    32101 gagaccgcga ttggaattgt tagagggaga aaacttctag caaatgtcat ctcatcaagt
    32161 gttgatgagc atgcaagatt tggtggggta gttccagagg ttgcctcaag ggctcatcta
    32221 gaggcgatgc aacctgcaat tactcaagct ttaaatgaat ctggagtaaa gctaaaagat
    32281 cttgatggca ttgcagttac tgcagggcca ggtctagttg gcactcttct tctaggtgtt
    32341 tcaactgcga atgctctctc acttgctctt gataaaccac tctatggagt caatcactta
    32401 gcggcccata ttgctgttga tctattaact gatgatagaa aaattagccc tgcaattgct
    32461 ctgctagtta gcgggggaca ctcatcaata ttgcaagtaa atgatttaac taaagatata
    32521 aaaccacttg gaagaactct tgatgatgca gcaggtgagg cctttgataa agtggcgcgc
    32581 ctgcttaacc taggttttcc gggtggtcca ttaattgata gagatgcaaa agatgggcga
    32641 ggtgatgcaa ttaattttcc tcgcggcctt aaccaagcaa aagatatggc agagcatcga
    32701 tttgatttct ccttctctgg tcttaagact gcagtttcaa gatatttagt agctaatcct
    32761 tcctataacc gctctgatgt ctctgcctct tttcaagaag cagttgttga tgtcttactc
    32821 gataaggcgg ttaaggcctg cctatcaagc ggaatccaaa ccttgataat tgctggaggg
    32881 gttggggcaa actctcgcct tagaagcctt gcagcttcca gatgcgagaa ggcaggcatt
    32941 gagctgcgac gcccccctgc caatctctgc accgataatg gggcgatggt ggctgccctt
    33001 ggctccctag ccatctcaag cgggattgag ccttcaaagc ttggcattca ggttgcctct
    33061 gagatcagcc cttcggtagc tatctgggcc tagagcctgc ggatttgaga acgggaatag
    33121 aggcctctag gcttggcgtt agcactcagc gggtgagagt gataaaacac ccgaagtagg
    33181 aaaaaactcg atgaagaagg agcaataacg atggccattg ccattaagcc gcttgaagat
    33241 cgtatcgtta tcaaaattaa cgaagctgaa gagaagaccg catcaggtct agttattcca
    33301 gatactgcaa aagagaagcc acaagagggc acggtagttg cagttggtcc aggacgattt
    33361 gatgatggag ttcgcgtgcc aatggatatc aaagttggcg atgtagttct ctatagcaaa
    33421 tacggtggaa ctgaaatcaa gcacggtggc gaggaatatt tagttctctc agcccgtgac
    33481 attcttgcag taatcgcgaa gtaattaaag aagaagagaa gataactaaa catgggtaaa
    33541 tcactgcaat ttgatgaaaa tgctcgtcgc gctatggagc gcggagtaaa tatcctggct
    33601 gatactgtaa aagtaacgct aggacctaag ggacgtaacg ttgttatcgg caagtcattt
    33661 ggcgcgcctt taattaccaa tgatggtgta accattgcta aggaaattga actatctgat
    33721 ccttcagaaa atatgggggc tcaattagtt aagcaggttg cggaaaagac caatgatgtt
    33781 gcaggagatg gcactacaac tgccacagtt cttgcccagg caatggtccg tgagggccta
    33841 agaaacctgg cagctggagc ccagccaatg ggaatcaaag ctggaattga agcagcagtt
    33901 gctgcagtcg ttgaaaagct aagagctaac tccaccaaga tctctgccaa agagcagatt
    33961 gcagatgttg caactatctc tgctcaagat cgcagcattg gtgatctaat cgctgaggcg
    34021 atggataaag ttggtaaaga tggcgtaatt accgtggaag aatcttcaac cactggtctt
    34081 gagttggaat ttactgaagg tatgcaattt gataagggct atatctcgcc atactttgtc
    34141 accgatcaag atcgcatgga ggcaaccctt gaggatgcct atatcttgat ctctgctggc
    34201 aaaatttcag ctcttgccga ccttctgcca attcttgagc aggttgcaaa ggcctcaaag
    34261 ccgctattaa ttattgctga agatgttgag ggtgaagctc tttcaacgct agttgtaaac
    34321 cgcatgcgcg gggtctttac ctcagcagct gttaaggcgc caggctttgg agatcgtcgc
    34381 aaggcgatgc tgcaagatat tgcagtacta actggcggcc aagttatctc aagtgaagtt
    34441 ggtctaaagc tagatcagat ttcaattgac atgcttggaa aggctcgcag aattgttatt
    34501 actaaagata acaccaccat cgttgatgga gctggaaaca agaaagatgt ggaaggtcgc
    34561 atcgctgagt taaggcgaga gattgaagca gctgactctg attgggatag agagaagtta
    34621 caggagcgtc ttgcaaagct ctctggcggc gtctgtgtta tcaaagttgg agcacatacc
    34681 gaagttgaat taaaagagaa gaagcatcgc ctagaagatg caatctctgc tactcgcgca
    34741 gccgttgaag aaggcatcgt ggttggcggc ggagcagctc tagttcatgc tgcaactgtt
    34801 cttgatggcg atcttggcca tgaaggagat tcagcagttg gtgttcgctt agttgctaaa
    34861 gcttgccaag aaccacttcg ttggattgca gagaatgcag ggcaagaagg ctatgtagtt
    34921 gtagcaaagg tgagaacgct aaagccaaat gaaggcttta atgcggcaac cgatgagtat
    34981 ggcgatctag ttaaagctgg agtaattgat ccagttaagg tcactcgctc agctcttgcc
    35041 aatgcggcct caattgctgc aatgtttatc actactgaag cccttgtctt tgaaactcca
    35101 gaagataaga aggaagaagc atcaggtcat ggccatagcc atggaccagg tggccatagt
    35161 cactaattta agtgataaaa agagtaagga aagagctctg atggaaaaca tcagggctct
    35221 ttctctataa actcaccgcc atggttagcg aatctctcaa tatctcaacc catatctggg
    35281 cgataactct tggtgtaatt ttcttggtaa ccctgggaga tttaatctgg gcttggttta
    35341 ggcgaaacac aattaccact ctaaaagagg cggcgatttg gaccggaatt tatgtctcag
    35401 ctgccattgc ctttggaatc tcactgcgca gctggggcgg gcagaccaag tcggctgagt
    35461 tttttgctgg ctggataaca gagtattcac tatcaattga taatctcttt gtatttctta
    35521 ttattttatc aaggctaaag attgaaaaag agaaagagca gctagttctt ctgctaggta
    35581 ttttgatggc ccttgtaatg cgcggaatat tcatagttat tggcgccgcc gttgttgaaa
    35641 gatttgtcgc cgtattcttc ttctttggag ctatcttgat ttggactgca tataagttaa
    35701 ttaccgaaga tccagaggaa gatgagtggc aggaaaatcg agtaattacc gctcttagaa
    35761 agcgcggtta ttccacattc accatcgcac tagttgctct cggaaccact gatctactct
    35821 ttgctcttga ctcaatccct gcaatctttg gtttaactag agatccctat attgtcttta
    35881 tggccaatgc ttttgccctt atgggacttc gccagcttta tttcttagtt ggcattctat
    35941 taaagcgcct ggtctatctc tctaaaggct tatcaatcgt cctaggcttt attggaatta
    36001 agttaattat tgaagctttg catggagtgg gaattgatta catctatgga gtaaaagttc
    36061 cccatgtttc attaacggtc tcacttgggg tgattatctc ggccttggca gtaactacgg
    36121 tgattagcct tagggcaagt gggaattttg aaaaaggtag agaataagta gttgcgttat
    36181 gcgctgtaag gactgctctg gcgcgatgcg cgagagatga tctcaagacg ctcatcttca
    36241 gttaatccgc cccagattcc atacggttct tgtgaggcaa gggcgtgagc tctacaggcc
    36301 gctaaaactg ggcatgaggc acagactgcc tttgcagcat tttctctagc gcgacgcctt
    36361 ggaccacgct ctccatctgg atgaaagaac atctcagttg gaagggagcg gcaggcgcct
    36421 tcaaattgcc attgccagtt atctataacg ggcttgggta ggcgagaggt ctctgacatg
    36481 ggcccaatat acaatcgcgc cataggttgt tcaagtactt tggggtaatt tctttgattt
    36541 cgggtcgcaa aagtaggtga taagcaccac tattggccca tggcccagcg ccccaaaagt
    36601 gccaacctta aggctggctt agaagatgaa cgtttaaccg tcgcctcagt agctcgcaga
    36661 ctcggcatag ctccagccac ccttagaact tgggatcgca gatacggcct tggtccatca
    36721 agtcatgttg agggtgagca tcgcagatac agcgctaaag atttagccag acttacctta
    36781 atgcgtaagt taattattca aggctactca ccatgtgatg ctgcagaggt agcgctaaag
    36841 agtagaacaa agagttttga atctttagct agcagggaag tagccgttga ggtagatgat
    36901 tgctcggatt tgattgaatc acttctaaaa gccgccagag tttatgatcg aagttttatc
    36961 gaagatgttc ttaggcagca gattgaaaag cgcggaatta tcaatacttg gaatgaactc
    37021 atagtcccag ttctaattgc cgtgggaaat gaatgggcag agcttggcac tggaattgag
    37081 attgaacatt tacttaccga aattataact agactaatgc agagctctaa tcaaacacct
    37141 gtgaatccaa ttaatccccg tccagtattg cttgcttgca taggtgagga gatgcacact
    37201 cttgcgctct ataccttggg ggcagctcta gctgagagaa acattcaatc ccaattttta
    37261 ggggcgagaa ctccaatttc tgcagtaggc gcaatggtga aaaagtcagc cccacctgct
    37321 gtctttctct gggcgcaatt ggctaagaat ggaaagagtg aagtggttgg cgatattcca
    37381 agagttagac cagcgccgcg aataattctt gggggtccgg gatggcgcaa aacttcaaag
    37441 aaatcgctag taaaagtcga ttgcctcgat agcgcgataa ctgaaattac gcgggcagtt
    37501 ggagcttaaa taccctagcc attaaggtgg agcggtgagt gagagcgtta atgagaaggt
    37561 cgcaatgctt ggccttacct acgatgatgt tctcctgctt ccagatgcat cagaggtagt
    37621 gccctcagag gttgaaacaa agacttttct tacccgctcg atccaattgg atatccctct
    37681 aatctcatct gcgatggata ccgttactga atcagcaatg gcaattgcta tggctaaagc
    37741 tggaggcatt ggaatcatcc atagaaacct cgctatcgat gaacaggtaa ctcatgtgaa
    37801 gttagttaaa ggagcaaatt taagagttgg agcagcagtt ggcgttggcg aagatggatt
    37861 tgaaagagct gaggcgctaa ttggcgctgg agttgatgta attgtggttg ataccgcaca
    37921 tggccatcac cgagcagttt tagatgcgat agtgagaatt aagaagcact ttcctagaca
    37981 agaggttatt ggcggaaatg ttgcaactag agcaggagct caagcgctaa ttaatgcagg
    38041 agcagatgca gttaaagttg gagtaggacc tggctctatc tgcaccacac gagttgttgc
    38101 tggagttgga gttccccaaa taactgcaat tatggaggca gcaaaggctt gtcagaaagc
    38161 tgatattcca ttgattgcag atggtggttt gcaatactca ggtgatattg ccaaggccat
    38221 tgtctcaggg gccgatactg tgatgcttgg atctcttctt gcaggctgtg atgaatcacc
    38281 tggtgagtta gtggaaagtg atggaagaaa attcaagatc tatcgcggca tgggatcact
    38341 tggcgcaatg caatcaagag gcgagcagaa gtcattttcc aaggatcgct atatgcaaga
    38401 tgatgtttta gctgaagata aattagtccc agaaggtatt gaaggaaaag ttgcttatcg
    38461 aggcagcgtc gcgcaagttg ttcatcaatt agttggcggg ctgcgctcgg cgatgggata
    38521 tgccggcgcg ccagatattg cagcgctaca aaaacgtggc cggctaatac aaattactgc
    38581 agcgggcctc tctgagagcc atccccatga tgtattagat gtggctgatg cccctaatta
    38641 ctatcggagt aaataatggt tgatatcgag atctcacctg gcaagagagc aagaactgcc
    38701 tattcatttg atgatatttc catcgctcct tcacgaagaa caagagaacc gagcgaagta
    38761 tcaattaact ggcagatcga tgcctatagc tttgggctcc cacttctagc agctccgatg
    38821 gattcagtag tttcgccaga gagcgctatc gctattggaa aattaggtgg ccttggagta
    38881 ttaaatcttg aagggctatg gagtagatat gaaaatccta agattcaatt aggggagatc
    38941 tcatctatcc cagcagagca agcaactagg cgtatgcagg agctctatca agctcctatc
    39001 agagctgagt taattaaaga acgcctagca gagataagag ctgcaggagt atgtgttgct
    39061 gcagcgctct cgccccagcg cacagcccag tttcataaga ctgtaataga tgccggtgtt
    39121 gatatctttg ttattcgcgg aacaactgtg agcgcagaac atgtttcaaa aactaatgaa
    39181 gcgctaaatc taaagaagtt tatttatgag ctagacgttc cagtaattgt tggtggatgt
    39241 gcaacctcgc agagcgcgct tcacttaatg agagcaggag cagctggagt tctagttggt
    39301 tttggtgggg gagcagctca taccactcgc caagttctag gaatctctgt tcccatggca
    39361 acagcagttg ctgatgttgc tgctgcaaga cgagagtatt tagatgaatc tggcgggcga
    39421 tatgttcatg taattgccga tggttcagtt ggaaagagcg gagatattgc aaaagcaatt
    39481 gcctgcggcg cagatgcagt gatgttgggc tctccacttg caagagcaga gtcagctccc
    39541 ggaagaggtt ggcattgggg gccagaggcg catcacaacg aacttcctcg tggcacaaga
    39601 gtttatgttg gaagttcagg atctttggaa gagattttgc ttggaccatc gcactcagct
    39661 gatggcagta tgaatctatt tggcgcacta cgccgggcga tggcaacctc tggctactct
    39721 gacttaaaag agtttcagag agttgaaata gttctaaata gagcataaag aaggcgagct
    39781 agtgcagagc aataatcatg tcttagtagt tgattttggc gcgcaatatg cccagttaat
    39841 tgctcgcaga gttcgccaag ctggagttta tagcgagatc atttctcata agtgttcact
    39901 tgaagaaata cttgcaagaa atcctaaagc gataattctc tcaggaggtc catcatcggt
    39961 ttatgttgat gatgcgccct taattgaccg ggctttattt gagcagaata ttccaatctt
    40021 tggtatctgt tatggctttc aagtcatggc gcaattactt ggaggagaag tagcaaagac
    40081 gggcttgagt gaatatggca gaactgcatt taactgcgat acttcagcaa agttattttc
    40141 aggtcttgat tcatcattta atgtctggat gagccatggc gatagcgtta ctaaggctcc
    40201 agaaggcttt caagcaacag gaagcagcgc ttcaacttcg gttgctgctt ttgaaagtag
    40261 cgatggaaag ttagctggag tgcaatttca tccagaagtt cttcatagcg aaaatggtca
    40321 ggaaattctg cgcaaatggt taattgatat tgttaaatgt aagccaacat ggacttctga
    40381 aaatattgta gaaaatgaaa ttattaatat taaatctcaa gttgcagata agcgagtgat
    40441 ttgcggcctc tctggcgggg ttgattcagc agttgctgca gcacttgttc agaaagctat
    40501 tggaaagcaa ttaacttgtg tttttgttga ccatggcctt cttaggcagg gtgaagctga
    40561 gcaagtggaa aaagactttg tggcagcaac tggcgttgaa cttatggttg ttgatgcaaa
    40621 gaagcggttt ctcgatgctc ttaaaggcgt aaaagatcct gaggagaaga gaaagataat
    40681 tggcagagaa tttatccgcg tttttgaaga tgctgctaaa aaattatcag cagatagcaa
    40741 aatagagttt ttagttcaag gaacgctata tccagatgtt gtggaatcag gcggcggaag
    40801 tggcactgca aatattaagt cgcaccataa tgtggggggc cttccagatg atttagattt
    40861 caaattgatt gagccacttc gcactctctt taaagatgaa gttcgccaag ttggactctc
    40921 tctgggcttg ccagatcaga tcatctggcg ccaaccattc ccaggtccag gcctagcaat
    40981 tcgtattgtc ggtgaagtta ctgaagaaag acttagaatt ttgcgccagg ccgacttaat
    41041 cgcaagagaa gaattacact tagcaggtct agatcaaatg atctggcaat ctccagtggt
    41101 attactcgct gaagtgcgca gtgtaggagt tcaaggagat ggcagaagtt atggccatcc
    41161 catagtcctt cgcccggtat caagtgagga tgcgatgacg gcagattggt cgcgagtgcc
    41221 ttatgaagta ctcgctaaaa tctcaaatcg cattaccaat gaagttagtg aagttaatcg
    41281 agttgttctt gatataactt ctaagccgcc aggaactatt gagtgggagt agaaagagtg
    41341 agaagtaagc gcaaagtaac tgttatcgct ctactttctg ctgtgctttt aattccaatt
    41401 gctccagtga gtgctgaaag taaagaaatt aacgtagctg ctccagcgaa gaaagttaag
    41461 tgcaaagtaa gtaaagcatc agcgcaatct cccatgcgat tacctgctcc tgaaaatgta
    41521 atgaagcgag caccaaaata tttaacatta caaactaatt gtggaaacat agttatcgaa
    41581 acacttgcaa aagcagcacc agtaactatt actgtcatgt cattgcttgc tcgaggtgga
    41641 tatttcgaca gaacactctg tcatcgacta accacagagg gaatttttgt tcttcagtgc
    41701 ggtgatccaa cggcaactgg ttcaggtggg ccaacgttta gatatcgtga tgaaaatctg
    41761 ccgctagcct ctgaaaataa ttatcctgaa ggcagcgtag ctatggccaa ttcaggacca
    41821 aacaccaatg gctctcagtt ctttctggtc tataaggaca ccacacttgg gcctaattac
    41881 accctctggg gaaaaatagt ttcaggactt gagattgtta aatacattgc tcaaggtgga
    41941 gtaaaaaatg gaggagtgga tggggctccg cttcgaacaa ttgggattga aagagctata
    42001 actagcaatt cttaatcagt acttacaacc ttaaatatct cagcaactct tcctgctgcc
    42061 aatccatttg cctcagcatt tctctcgccc cactgcctaa ccatcgattc aagctctggg
    42121 ttatgagcag tctggctgac atgggcctta agcgctgcaa tctttttgtc aattgtttcg
    42181 gtgatatcga tgaagtgatt tggggttggg gagccggtaa taaagacctc tcttactcgc
    42241 catggctcaa ttccttctgc ttttaattct gggaaggcgt aaggatttct tgcatcggga
    42301 tagattgcct gaatcgcgct ctctcctgct gcaagatgat ctggatgact tgagtaaatc
    42361 cgttcccaat ttcgctcggg tgattggacc accattcgat ctggttttgc tatacgaatg
    42421 gctttaacga tatctcgccg aagttcaata gttggaatta gtgaaccatc tcggtaattt
    42481 agaaaagtta tctcactaac gccaagggcg gcccctgcag ctctctgctc tcgctgtcta
    42541 accttcgcca tttcttcaac gggaacatca gattcttctc cgccttgatc gccatttgtg
    42601 atgatgcagt aagagacttg gattccagcc tctcgccact tagcgatgct tcctcctgca
    42661 ccaaagtcgc aatcatcagg atgggccata acgaccagaa tgcgcttaat ttcttcatct
    42721 gcaattggag tcatctgcct atcctagact taccactatg gcagcgctta atgaagataa
    42781 attgttagca gggttaaacc cgcagcaagc agcagctgtc acccactctg gatctcccct
    42841 ccttgttgtt gcaggagcag gttcgggaaa gacccgggta ttaaccaggc gaattgcata
    42901 tctgatggca gcaagaggcg ttgatcctca cgagatattg gctattacct ttaccaataa
    42961 agcagctggt gagatgaaag aacgcgttgc acaattagtt ggaaatcgcg ccaaaataat
    43021 ctgggtttca acctttcact cagcatcggt gagaatcctt cgccaggagg ctggaaggct
    43081 gggatataaa tcgacgtttt ctatttatga tcaagcagat tcgctgcgct taattaccct
    43141 ggtaatgaga gatctaaatc tagatcctaa gcgatataac cctagaggag ttgcatcggt
    43201 aatttctggg gctaaaaatg atttattggg cccaagtgat tatagaaatg ccacccaaaa
    43261 tagctttgaa gaggttgttg ctgagatcta tgcgatttat caggccaggt taacaagtgc
    43321 taatgcaatg gactttgatg acctaattat gaaaacagtt gatttacttc agaagtttcc
    43381 agaggctaga tctcgttatc gccatcgctt taagcatatt ttggttgatg agtatcaaga
    43441 tactaatcat gctcaataca tcttaattag agagcttgtt ggaagtgata gagagggaat
    43501 tcctgcagct gagctctgcg tagttggcga tgctgaccaa tcaatctatg gctttagagg
    43561 ggcaacaatt agaaacatca tggcatttga agaggattat cctgacgctc agacaatttt
    43621 gctagaacaa aactatcgct caactcagaa tattttaagc gctgctaatg ccgttatctc
    43681 acttaatgag gcgcgcaaag agaagaacct ctggtctgat gcaggaagcg gagccaagct
    43741 agtgggcttt gttggcgaga atgaacatca tgaagctgaa tacataagag atgaactctt
    43801 tagattagag agagaaggta tttcaaactt tggtcagagc gctatttttt acagaactaa
    43861 cgcccagtca cgcgtatttg aagaggtgtt tatgcgcgcc actattcctt acaaagttgt
    43921 aggaggagtg cgcttttatg aacgcaagga agtaaaggat ttacttgcct atcttcgagt
    43981 attggtaaac cctgatgatg aggtaagcat gcgaagaatt atcaatactc ccaagcgcgg
    44041 ccttggcgat agggcccttg aagctatcga tgattatgca aagactgaag gaattacatt
    44101 ctggcaaggc ttaaatagag caagtgctgc tggccttgga gcaaaggcat catctgctgt
    44161 tgcggagttt gttgcaatga ttgcagctct tcaagtatta gttgaagcca atcgaccacc
    44221 agcaatcatt gctgctgcag ttcttgagca atcagggtta ttagacgagc tccgtgatag
    44281 ccatgatcca caagatgagg tgcgaattga aaatcttgaa gagttagtag cagttgctga
    44341 ggagtatcaa gagcgagaag ttgatgaagg tcaagagatc tcacttgctg gatttttaga
    44401 ggatgtggca ttagttgcag atgcagatga gattccagag ggtgaagatc atggcggtgt
    44461 tgtaactctt atgacgctac acactgctaa aggtttggaa tttccaaccg tatttctcac
    44521 cggaatggag gaaggaattt ttcctcactc aaggacgcta ggtgagaaga aggagttaga
    44581 ggaggagaga aggctggcat atgtcggact aacaagagct agagaaaggc tctatctttc
    44641 gagagcggaa tatcgctcat cttggggagc gcctaattac aaccctcctt caagattttt
    44701 agatgaaatt cctgatgcct tgattgattg gaataagagt gagagtggat acatctcatc
    44761 tccaatgacg agacgaaaat atgcaccaag tgcgctacca aaagcagcga ttaagtcaaa
    44821 caccttgcaa cttaatatcg gtgatcgagt aagccatgac acatttgggc ttggcactgt
    44881 gattgcgctc tcaggagagg cagagaaagc agaggccaca atcaattttg gtagctatgg
    44941 agataagcga ttgttgctcc gatatgcccc agtagataag ttatgacccg tggacctctt
    45001 tgaatatcaa gcccgtgatc tatttgaggc caactcaatc ccagttttga aagcaaaagt
    45061 tgccacaact gctgcccaag caagagatgc agcgcaggaa ataggtggga aagttgtagt
    45121 caaagctcaa gtaaaaattg gtgggcgcgg caaagctggt ggggtaaagc ttgctgaaaa
    45181 tggcgaagat gcttatgtaa aggcagaagc aattcttggg atggatatca agggccacat
    45241 cgtaaagaaa gtaatgattg ctgctgctgc tcctattgag agcgagtatt acttagcaat
    45301 tcttcttgat cgctctaatc gctctttttt agtgatggca tcggttgcag gcggaatgga
    45361 tattgaagaa gttgcacata aaactccaga gaaattagca aaagttcata ttgatcccaa
    45421 tgagggaatt agccaagaaa aagctttagc gatagtgcga caaggtggat ttggtagtga
    45481 tgttgaaaac caagtcgccg atgtattagt aaccctttgg aagacttttc aaagctccga
    45541 tgccactttg gttgaggtta atcctttagt gaaaactgtt gatggcagag taattgccct
    45601 tgatggaaag gtcacacttg atgacaatgc agcattccgt catcccgaat ttgaagccct
    45661 gattgatcat gaagcagccg atccacttga agcactggca aaagagcgcg atctaaatta
    45721 tgtaaagctc caaggcgagg ttggcgtcat tggtaatggg gcaggacttg tgatgagcac
    45781 tctagatgtc gtggcatatg caggcgagaa gtttggcgga gtaaaaccag cgaatttcct
    45841 tgatattggc gggggagcat cagctcaagt aatggcagat ggcctatcta tcattcttgg
    45901 agacaaagat gttaaatcag ttttcgtaaa tgtttttggc ggtattacct catgtgacgc
    45961 agttgctaat ggaattattc aagctcttga aatgctaggc gataaagcaa gtaaaccaat
    46021 agtggttcga cttgatggca ataatgttat tgaaggaaga gcaattctaa acaaagcaaa
    46081 tcaccctttg attcagcagg ttgagagtat ggatggagca gctgcaagag ctgcagagtt
    46141 ggcagctaaa taatggcgat ttttctaact gagaagacaa aagtaatcgt tcagggaatt
    46201 actggctctg aaggaagtaa gcacaccaga aggatgattt tgagtggaac aaacattgtt
    46261 ggtggcgtaa ctcctggtaa gggtggtcag gtagttgaga ttgaaggaaa atctcttcca
    46321 gtatttaatt cagtaaaaga ggcaattgct gcaactggcg ccgatgcctc agtaattttt
    46381 gtgccaccaa aatttgccaa gagcgcggta attgatgcaa tagatgctca tctgccatta
    46441 attgtggtca tcactgaagg aataccagtt catgacacag caagttttta tgctcatagc
    46501 ctcaaggttg gcaagtcgcg aatcatcggc cctaattgcc caggattaat tagcccgggt
    46561 aaatctaata ttggaattac gccagctgat atcacaggcc ctggaagtat tggtctagtt
    46621 tcaaagtcag gaactctcac ttatcagatg atgtatgaac tacgagacat tggttttaca
    46681 actgctgttg gaattggtgg agatccaatt gttggcacaa cacatataga ttgcttaaga
    46741 gcttttcaag atgatccaga gactaaggca attgtgatga ttggtgaaat tggcggggat
    46801 gctgaggaga gagcggcagc atttattaaa gagtatgtga caaagccagt agttggatat
    46861 gttgcaggct tcaccgcacc tgaaggaaaa acaatgggac atgcaggagc tattgtctct
    46921 ggctcttctg gaacagcaga ggcaaagaag gtggcgctag aggcagctgg ggttaaagtt
    46981 ggaaagacac caagtcagac tgcagaactg ctccgagcaa ttcttaaagg ataagcaaga
    47041 ataaagagat gttgccactg ctgaaagttg ctttcggcca aggcgttaga ggcataactt
    47101 taattcttct tccgctggcc tttatctcac ttattacctg ggcgacagct ggtagctcta
    47161 ctggaaatac cgcagatcca atgcgcgctg caatctggtt ttttcttatt gcccatcaca
    47221 ttccactgga tctatcactt tcaaatgaaa ctatctctgg tcgcttaacc ttctttccaa
    47281 tcggagcttt gataattcca tttcttgtga ttaaaaattc cttgctccga atgagcgaaa
    47341 ggcttggcac aaatagatca agtgataaga gagctcaaat attggcactt tcctttgctt
    47401 atagcctttt aggaactctt ctttcacttg cctcacttgg aagcacagta aaggcgccct
    47461 tctacattgt ctttccaatt ctttgtattg tgtcagcagt ttcaggattt attgcaagca
    47521 atcttcttcc tgatcacggt attcaatttc cctggcagcg cgctcttaaa ttagcaacac
    47581 ttcttttatt ggctctaatt ggatttgctg gattagtttt tagcttctcg ctcatttggc
    47641 actttgatac tgtacttgac ttaacaagag ttattgagcc tggtgtcttt ggtgggttgg
    47701 cattcttatt tattcaaatt ctttacttgc caaacttctt tatctcaact cttagctaca
    47761 tcgctggaag tggcgtggta ataggcaatg ggacttggtt aaatcctttt gtccatcgcc
    47821 ttgatgaaat tccagcaatc tcactacttg gtggattgcc agtgggtgct cagccgctct
    47881 ttatattact gacattagtt attgttggac ttggaattgt tatgggtaaa tatggagtga
    47941 gtaaatatca agaccaggtt gagctacgta gatttttcct ggcccttttt ggctttatag
    48001 ctgggctgac tttgtttatt gctcgggcat caagtggcga gctcttatcg ccaaatcttg
    48061 aatcagttgg tccccattgg tatttaatgc ccctagctct tagccttgaa attggcctgg
    48121 gagtggcaat atttctatat acgccagtag ttatttcgaa agtgcgcaac tttagggctg
    48181 cgaaatagcg ctacttcttt cgaactagaa taggaccatg gcccaagcaa aagtattaga
    48241 tggcgttgca gcggccaaag ctattaggca agaggttgct ctgaaggtag caaagctaaa
    48301 aaataggcct ggtttagcaa cagtattagt tggagaagat cctggttcac gctcctatgt
    48361 agatggaaaa catagagatt gtagccaagt aggtattaac tcaatacgaa tcgagctgcc
    48421 acaaactgtt agccaaaccg agttattaag tgaaataaat aatctaaata gctctaaaga
    48481 ttgctcgggt tttattgttc aacttccact accagcccac attgatacaa ataaagtttt
    48541 aagtgcaatt gatccagata aagacgcgga tggacttcac ccaataaacc taggcaagct
    48601 agttctttct caaggcgggg taattccttg cacacctaga gccattattg agcttcttgg
    48661 acgaaatgaa atttctctaa gcggtaaaga ggtagtaatt attggtcgcg gcacaactgt
    48721 tggaagaccg ttaagtcttt tgctttcaag caaaggcatc gatgccaccg taacaattgt
    48781 tcatagcaag agtagcgata tcaagtctca tactaagcga gccgatgttg ttatagctgc
    48841 ccttggaaaa gctcactttc ttaccgcaga tatggttaaa gaaggcgttg ttgcaattga
    48901 tgtgggaatt actagaactt cagaaggttt agtgggagat attgatcctg aagtgatgaa
    48961 aatagcgagc gcgtttgctc caatgccagg tggggtaggg ccaatgacaa gggcgatgtt
    49021 attgtctaat ctattagcgc tacataagga ttaaagccaa ggtgaatatc aagcaaattt
    49081 cctatgccct ggctctctct ggggtattaa ctggagcgct gctttcagtg cgaatcggag
    49141 cgctaattat tgccgctggc ttcatactct ttctaagccg agatcttgcc tcgatgcgtc
    49201 caattcagaa agtgattcct atcgcactgg ttatcgcgct aatcgccatc gcattggcgc
    49261 ttcctagagg atagaaggta ggtttcgcgc catgagtaga tctggaaaag taacggtagt
    49321 cggagctggt ttctatggtt caaccacagc gctacgtctt gctgaatatg acatctttga
    49381 aagcgtcgtt ttaacagata ttttggatgg aaaacccgaa ggtcttgccc ttgatattaa
    49441 tcaatcacga tccattgaag gttttgaaag cagagtaatt ggagcaacaa ctactgctga
    49501 cggcaaagga tatgaagcaa ctgcaggatc tgatgtagta attattaccg caggacttcc
    49561 tagaaaacct ggaatgtctc gaatggatct aattggtgtt aatgcaggaa tcgttcgagg
    49621 agttgcagaa aatattgcta agcactcacc aaatgccgtc gtcattgttg tttctaatcc
    49681 actagatgag atgacagctc ttgcgcaaat tgctaccaag tttccttatc aaagagttat
    49741 ggggcaagca ggaatgcttg atactgctcg ctttactcac tttgtggcag aaaaactcaa
    49801 tgtgccagtt gcatcagtaa agacattaac tctaggaagc catggagaga cgatggttcc
    49861 aattccatct cgctgcacag tagatggcgc tgcgctatct acaaagttaa gcgatgctga
    49921 aattcaagag ctagtgacac ggaccagaaa tggcggggca gaagttgtcg ccctcttaaa
    49981 gacgggctct gcttactacg cgccatcagc tgcagctgct cggatggcaa aagcagtaat
    50041 tcaggactct ggtgaggtaa tgccagtctg tgcttgggtg gatggccagt actcaattac
    50101 tggggtttat ctaggagttg aagcccaaat cggtagagct ggaattacta aagttgttga
    50161 aagtgattta actgcaagtg aattagctga gcttaaagtg gctgctgaag cagttcgcgc
    50221 caagcaagca gatgtaaaag atctataaac aggcaaacat caagagaggc agatagatag
    50281 atgagcaaga taaaggtgca aggcagcgtc gtcgagcttg atggcgatga gatgactcga
    50341 atcatctggg actttattaa aaagcagttg atcttgcctt atcttgatat caatttggaa
    50401 tattacgatc taggtatcca gcaccgtgat gccacagatg atcaagtaac tattgatagc
    50461 gccaaagcca tccagaagca tggagtgggc gttaagtgcg ccaccatcac ccctgatgag
    50521 gcaagagtaa aagagttctc attaaagaaa atgtggaaat cacctaatgg aacaattcga
    50581 aatattctag gtggagtaat tttccgtgag ccaattatta ttaagaatgt tccaaggcta
    50641 gttccgcatt ggagtaaacc aattgttatt ggtcgtcatg cctatggaga tcaatatcgc
    50701 gcaacagatt ttagagttcc aggtcctgga aagctcactg taactttcac accagaagat
    50761 ggctctgctc caatggagtt caatgttttt gatttcccat catccggtgt tgctatggcg
    50821 atgtataacc ttgatgattc aattcgtgat tttgctagag cttcatttaa ttatggaatc
    50881 gctcgaaagt atccagttta tctttcaact aaaaacacta ttttgaaagc ttatgatgga
    50941 cgattcaaag atatctttgc tgaagttttt gaaaaagagt tcaaggctga gttcgataag
    51001 ttaggtctgg aatatgatca tcgtctaatt gatgacatgg ttgcaacctc tcttcgctgg
    51061 gaaggtggct atatctgggc ttgcaagaac tatgacggcg atgttcagtc agataccgtg
    51121 gcgcaaggct atggctcact tggtcttatg acatcagttc ttatgtcacc agatggcaga
    51181 actgttgagg cagaagctgc tcatggaaca gttactcgcc attaccgcga tcatcaagct
    51241 ggcaagccaa cctcaactaa tccaattgcc tcaatctttg cttggactca agggctacaa
    51301 caccgcgcaa agcttgataa cactccagag ctaggcaagt ttgctagctc acttgagaga
    51361 gtctgtattg aaacagttga gagcggaaag atgactaaag atcttgcgct tctaattagt
    51421 agcgatgctc cttggttaaa cacccaagag ttcctggcag caattgatga gaatctgcag
    51481 aaggaaatga agtaggagaa attaggaaat aaaaaagccc ccggttatgg gggctttttt
    51541 aatgcgtttt aactacttaa tacgcttgcc atctgcagtg ctaaatagat ggacagcctc
    51601 agccttggct gtaacagtga cagtttgtcc tggctttgga ggattctttg ggtcccaacg
    51661 aagaatgatt tgagctcctt catcgctagc attagcaaac ttcacatttg aatcagctgg
    51721 cttgccatag acgaatgcat cagcgccaag ttcttcaacg acttcaacaa caacatcaaa
    51781 gcccttactt gatgagctaa atccttctgg gcggatacca acagtgactg aagcgcctgc
    51841 agatgatgga acatctacat ctaggtttcc aagctttgct ttaccgccag atactggaac
    51901 cgttagtagg ttcatagcag gtgagccaat aaagcctgca acgaaagcat ttgctgggtt
    51961 gtcatagaga tttcttggtg tatctacttg ctgcaaaagt ccatctttaa gaactgctac
    52021 gcgatcaccc atggtcatag cctctacctg gtcatgtgtg acataaacag tagtaattcc
    52081 aaggcggcgc tgtagcgctg cgatctgggt acgagttgca acacgaagtt tcgcatctaa
    52141 atttgaaaga ggttcatcca ttaagaagac ctgaggttcg cgaacaatgg cgcgacccat
    52201 agcaactctc tgacgttgac cacctgatag cgcttttggc ttacgctcta ggtatggttc
    52261 aagatctaat agtttggctg catctaaaac gcggcgttca cgttcttctt tagcaacgcc
    52321 agcaatcttt agagcaaagc ccatattttc agcaactgtc atatggggat agagcgcata
    52381 tgactggaag accattgcaa tatcgcggtc tttaggagcg acatttgtta cttcccgatc
    52441 tccgatataa attgcgccgt tatcaatttc ttctaatccg gcaagcattc gaagtgatgt
    52501 tgactttcca catcctgaag gtcctactaa aaccaaaaac tcaccatcat tgacggtgag
    52561 atttaattta tcgactgcag gagcgcttgt tccaggatag atgcgtgatg catctttaaa
    52621 tacaactgat gccatttctt tttctctctt ccttctccgg gcaggtacgt gcccgacgat
    52681 ccgttggatg aagcaagtcc acttacgtgg ctgggcgaag agtagcgccg agttgagcgt
    52741 ggggaaaggg gtcatctggc gtgatttagc ctcaaaggca ctacttctta taccctagag
    52801 ccctaatgga ccctggtggt gctaaccctt ttacccttca agacctagct ttagatatct
    52861 cggtcgtagc cttttttatc gcccttgggg cgctctttgt cgctgctgaa attgccctga
    52921 tctcacttag agagggtcag ttaaaggccc ttgctgaaaa gagtaggcga ggggcaaagg
    52981 ttgctgatct ggcctcacac ccaaatcgct ttctagcggc agttcaagtt ggagtgacct
    53041 tcagtggatt tctctcagca gcctttggcg cagatcgcct aggagattac ttaattcctg
    53101 cacttgaaga tgctggattg agcaattcaa taagtgaaac catttcttta gttggcttaa
    53161 cacttgttat tgcctacttc tcgctagttt ttggtgagtt ggtgccaaag cgagtagcgc
    53221 ttcactctag tgaaaagatt gcactctttg tggttactcc ggtatctgtg actgctcgct
    53281 tatttcgtcc tttaatttgg ctactttcac actcatctga tttagtcttg aagctatttg
    53341 gaattgatcc aaaggcatct cgcgaagata tctctgaaga agagctagtg gatctagtaa
    53401 ctggccataa agcgctaaca gaggaagagc gcgatattgt tgaagaggtc tttaatgcaa
    53461 gtgagcgtct cgttcatgaa gttatggtgc caagaactga agttgatttc cttgatgcca
    53521 atttactagt cagtgatgcc attttgatgg cagttgagaa ggctcactct cgatatccag
    53581 tggttcgggg aagtagcgat gaagtcattg gctttatcca tgtgagagat cttcttgatc
    53641 ctgagcattc aggaaaaggc atagctatta tgaatttggt gcgtagcatt atgttcttgc
    53701 ctggaactaa aggtgttctg cctgcgctat ctgagatgcg cgctgctcgt cagcaaattg
    53761 cgatagttct tgatgaatat ggcggaactg atggaattgt cactttagag gacttagttg
    53821 agaccttaat cggtgatatt catgatgaat atgatggcca tgaggttgaa gtctcaaatc
    53881 agagtcgcac tggtgactat gaaattgatg ctctgatttc actcgaggat ttagctgagc
    53941 agactgggat tgaaatccca gagggtccct atgaaactct aagcggctat gtgatgcatg
    54001 agctgggaag aatcccgcaa ctgcatgatg ttatccatct agccgggatg agactgacag
    54061 tcttgagttt agagggcaag cgcgttggga ctgtcttaat ctcaaaatta catactcaag
    54121 ataaaaattg agaagatagg gccatgaagc gcgtcctatc tgggattcaa cccacagcag
    54181 attcttttca cctcggtaat tacttaggtg cggtcaagca atgggttgag ctgcagagcg
    54241 agaatgatgc tttttattgt gtcgttgatc ttcacgcgct aacggttgaa acagatccac
    54301 aagtattaag gaagcgaacc cttcttgcag cagcgcaatt aattgctctt ggccttgacc
    54361 cggataagtg cacgctattt attcaatctc atgtgccaga acataaccag cttggctggg
    54421 tacttgaatg catggcggga ttcggtgaag caaatcgaat gacgcaattt aaagataaat
    54481 cacagaagag tggtgcggat agaacagttg ttggcttatt cacctatcca attcttcaag
    54541 cagctgacat catgctttat caagcaaata gagttccagt tggcgaagat cagagacaac
    54601 atattgaatt aacgcgcgat ctaggccaga gatttaattc aagatatggg cagaccttca
    54661 caattcctga ggctcacatt attaaatctg cagcaaagat caatgatctt caagatccaa
    54721 atgccaagat gagtaaatca tctgcgtcaa attctggagt aatcgatatc ttggattcag
    54781 cagaagttaa tgccaagaag ataaagagcg cagtaactga tgctggtaaa gaaattaagt
    54841 ttgacgagaa ggaaaagcct gggatatcaa acctcttaac tatccactca gcccttagta
    54901 atagagcgat ctcggatatt gaaaatgagt ttgctggcaa aggctatggc gattttaagt
    54961 cagctgtagc tgaggttgtt acctcatact ttgccccagt taagaagcgc accgaggagc
    55021 ttttatctga tgaagctcac ctattgaaaa tcttggagca gggaagacag aaagctagag
    55081 ctgttgctgc agagacttta gctaagacat attcagctct agggttggtg ccaagctctt
    55141 ttaaggacaa gtaagtcatg agatctagag cgcttctgat agcgcttact atttcattat
    55201 tttctccatt cacgacagca attgccgctg aaccaaaagt tgctatcgct tatgacattg
    55261 gatttcttgg agataactcc ttcaatgacg cagttcacct agctcttaaa gctgcgaaga
    55321 aaaagtatgg actagttgaa ccatttgttc gcgaggttcc aacaaatgga actgcagtag
    55381 atcgcctcac acggttgcga tttttagcca aaagcggcta caccctaata attgctgtcg
    55441 gcccggggta tagacagaca gtagcaaggg tttcaaagga atataccgag actcagtttg
    55501 caattattaa tgacaaatct attgcacagt taaatgtctc aaatatctat tttagagaag
    55561 accatggtgc ctatcttgca gggatagcag cagcgaatct ttcaaagcgt aaatcaattg
    55621 catttattgg aagcgaacct gaactacttg agagctttac aaaaggagca agagtcaatt
    55681 ttccaaaggt aaaaataaag aatgtctcct atccagattc tgttgaatct ttgcgaagtc
    55741 aattaagtgg agtagatatc gcttattcaa cttgggatgg agacccttca gttgttgcgg
    55801 ttataagtga ggattttgct gggaaaatta aattgatttc agagatccca gatcaatatt
    55861 ttgctcaaat tccacagaca aaatctattg ttgtcgcaac ggtttcaaag caaatgtcta
    55921 aacctatttc gcagttagtt tcagcagcac ttgaatctcg ggcaattatt gacgttctcg
    55981 atgatgctga agggatttat ggccgggaat acaacgcaac caatggagga gttgggataa
    56041 cctttaacat ttcagtaaat agctccctta agagtcaact taggcaagct cttattcaac
    56101 taaaacaggg ataactacca gccggggtgt gcgctcttac cgccgtcaaa atcaataatg
    56161 cttccagagg caaaactaaa ggtttcacta gttattgcta gtgctagttc ggcaacttct
    56221 tcagcgcttc caatgcgccc tgcaggcact gtattagtcc aagaggaaaa accctccggc
    56281 cacctgcttt ctaacttatc atctccaata agacctaatc tgattccatt aatccgcatg
    56341 ggcgcaaggg atagagcaag tgatcgagta acagactcta aagctgactt tgaagcgcca
    56401 tagatttcat gacctgatct ggaatttaat gcttcaattg atgaaatatt aatggctaat
    56461 gtagcgcctt ttgattttgc cgcgagaatt atctcaattg gagagagaag atttacttga
    56521 aacatctctt gagccttagt cgatgaataa tgcttaagat caaggattga ttgatcagcg
    56581 gcattattga ttacacaatc aattgcgcct atttgctcaa ctaactcagt gccacttgaa
    56641 gctttagaaa gatcctttct gaatacttca ataccatcaa tgcctttaga actatttact
    56701 tggccaacca ccctccagcc tgcagcgata aaggttttgc aaagcgcgct tccaatgagc
    56761 ccgctagcgc cggtaataag aatagatttc tgcttactca tgaaatagct ccggtaggtt
    56821 ggcaatgttt gggaattgag agattaactc atctctgcca gctaattcca tagttgaaaa
    56881 tgctggagct agaaaacagc tcaccagtga ataagaccct aaagactctg ctgccatcca
    56941 ggttttagct ggcacttggt aggcaagatg ctgcccttct tgctcgagtc gatggctgac
    57001 aagttgtccg ttttcaatgc tgtgaagcag aaggggatcg ccagataaat gaagccagag
    57061 ctcggcctgc ggcaggcgat gccaggctga gaaatcgggg gagaccacca taaaataaat
    57121 tgcattgctc aattcatctc gatagatcgt tgaatataaa ccaccttcac cttcaagggg
    57181 ctgcaaatca agtttttcta tcaattcatc tttgttcatt aaattccttg tgcaaagtct
    57241 ttactaccac atcaacttct ttcgataatt cagagaagtc aatcaaggtt gacttgctat
    57301 ctgctattac ttgtttccca tcaacccaaa catcgctcac atcgccgcgt cctgcagcaa
    57361 agacaagctg ggcaaatacg tcgtgaagcg gaattagatg cgccttgttt agatctattg
    57421 aaatcaaatc tgcgcttttg ccaacttcaa tacttccaat tgaactctca agtcctaaag
    57481 cttttgctgc ctcaattgta gccatgcgaa aaatctcact tgcccttaaa ttttctggac
    57541 cattgctttg ggcatatagg ttggcagcca ctcgcatgac tgcccacata tctagatcat
    57601 tacttgatga gcaaccatcc gttccgagtg caacacaaag atcgctactt tgatattttt
    57661 taatgtctgc aatcccactt cctaatttga gattgcttcc agggcaatgg gcaactgcag
    57721 ctcctgccct ctccagtaat ttaatgtctt cttctgatag gtgaaccccg tgccctaata
    57781 aggtatgaga atctaagatt ccagtttctt gcaatatttc agttggagac ttaccgaaac
    57841 gtttagcaac atcatcattt tctccaagat tttcagagat gtgaatactt atcgaagctc
    57901 ccatctgctt tgctagcttt ccaatttcaa ctaggtgatc aggcgagacg gtgtaggttg
    57961 aatgtggcat taggtagggc ggaacatctg gcccgccaac atcttgaagt acttgctccc
    58021 attttttggc tatctctaat ctggcttccc aatcaaggcc atcaagacca gtgaaatcaa
    58081 agaagagtgg accggtgatg tgtctgattc cagtacttgc tgcgccttta tgggctgcat
    58141 ctgggtagag atacatatca agagtagttg tggttcctgc aaggagtgat tcaagtgcgc
    58201 caagttgggt tccaagtgca acattttttg gattcataat taatccttca gctgcccaga
    58261 ctcgctctag aaatccttga agatttactc cttcggccca accgcgcagt aatgacattg
    58321 ctagatgggt gtgagtattg attagtcctg gagcaatcaa ctttccttta gcattatagg
    58381 tattagcacc ttttaaacta cctgcgccct gaattgcaac aatcttctta tctttaattc
    58441 caatggcaca atctttaagg actgtatttt tgctatcaga tgtgacgata taggttgcgt
    58501 tctctattac taaatcaaaa tcgctcactt ttatgccaaa acctttagcg cgccttcaat
    58561 aactgttaaa gctttagatt ttcctagatc cagggcctta tttatttcat ccatggtaat
    58621 ctcggcctca cttaatcctg ctgcgggatt aacaactagc gcaatgctgg cataagcaat
    58681 accaagttct cttgccaaac tcacttcagg acatcctgtc attccaacaa ccgtcgcacc
    58741 agcaagtgct gctagtcgaa tctctgaagg agtttcaaat ctaggcccat taaatccggc
    58801 atagactcca ttttcatgaa gcaaaattcc tgactcctta gcgctttggc gaagagcgct
    58861 ttgtattttt gcatcatatg ggcgagtaag gtcaacatgt tgaacgccag gttttgatcc
    58921 atccgagaag gtgtgctgcc tacccgaggt gaagtcaata aagtcatcaa ctaggcaaag
    58981 atcgccggga gatagctttg gattgattcc gccaactaca tttattgcaa ttatctgctc
    59041 aacacccaaa tccttcatag ccttgatatt ggctcgataa ttaattaaat gtggcgggat
    59101 gctgtggcca actccatggc gggttaaaaa tataaggcga gcgccgttcc attccccact
    59161 tctaatttga gctttcccat agagggtgtc taactctcta gattcaccat cttttagtgc
    59221 aggaaggtca tagaagccag taccagctat taatccaatt atctttggca tcgaatcaag
    59281 atgccaggcc ggccatcaat cgaccaatgg tatttctatc agcttcttta gaagtaaatg
    59341 ttgcaacaat ttttccattt gaaattacgg ctactctgtc tgccagtgaa agaacttcat
    59401 caagttctgc cgaaaccaat agtactgcag ctccagcatc acgagcagcg atgagttggt
    59461 tatgaataaa ctcaatcgag ccaacatcaa cgcctctggt aggttgagct gcaacaacca
    59521 catctaattg ttggcttaat tctctcgcaa caacaacttt ctgtttgttg cctccagaca
    59581 atgatccggc agacaatgaa gcatttggtg ctctaatatc aaatttttca acgagtgact
    59641 caccattgtt ggcaacttgc cctagatttc taatccatcc cttagcaaat ggtgcttgat
    59701 caaatcgatt caaaacgaga ttgtcagcaa ttgaataaga cgtgactagt ccatgttttt
    59761 ctcggtcttc aggaatatga gaaacacctg ccagatgtgc tgcccgggga ttcatattgc
    59821 caatacttat tcccttgatt actacttcac cacttagtcg aggccgcatg ctgcaaatag
    59881 cctctactaa ttcacgctgg ccattaccct caactcctgc aattcctaga atctctccag
    59941 catgaacctg cagagataga tttcttacag cttcaagctt cctatcatct ttaactgaaa
    60001 ggtttctgac atctagcaac acctccctag gttttgctac ccctttttct actcttagaa
    60061 caacttctct accaaccatc atttgagcca gttttgattc actactctcc tttggggtag
    60121 tggtgcccat caattttcca ccgcgaagaa cagcaattcg gtcagcaact gccatcactt
    60181 cacgcaattt atgggttatg aataaaatac caaccccacg cttcgcaagg tttcttaaaa
    60241 cttccaatag ttcatcagtt tcttggggcg taaggactgc cgtgggttca tctaaaatca
    60301 ataatttcac atcacgccga agcgctttaa gaatttctac tcgctgagcc atgccaacgg
    60361 gaagatcttc gataattgca tccgggtcta cctcaaggcc atacttttca gaaagactta
    60421 ttgcctctgc tcttgccttt ttgatgttga ccactccaaa tcgctttggt tcatcaccaa
    60481 gaatcaagtt ttccgtaaca ctcatgacag gaacgagttg aaagtgctga tgcaccattc
    60541 caaccccact tgcaattgct gctgcggtat cgccactatc catgatgtgg ccatcaattt
    60601 ttatagttcc agaatcaggc ttaacaagtc caaagacggc tttaacaaga gtggacttac
    60661 ccgcgccatt ttctcccaaa agggcaagta tttcgccttc cttgacactt agagagatgt
    60721 tttcattagc gacgactgaa ccaaaacttt tgcttactcc ctgaatttct agcaaggttt
    60781 tcatgacttc tctttctcat atggaattcc ttcagccgca ggggggcgaa cttttccagc
    60841 ggagattgct aagacaagaa ttgttagtat gtaggggagc atgtttatga actgaggcgg
    60901 aatctcaaca acctcatcaa tcattagctg atttgttacg gcttgagtca atccgaagaa
    60961 aagcgctgca gcaaatgctc caagtggatt ccaccttccg aaaatcatga ttgcaagagc
    61021 agtgaagcct ttacctgccg tgaatccacg ctcaaaaatt ccaactgctt caagcgcaag
    61081 gtatgaacca gcaagtccac caactgcacc ggcaatcgtt acatttatat agcgaagctt
    61141 tgttacagag actcctgcgg tatcagcact agagggatgt tcaccaacag cccttgaacg
    61201 caatccccat gaagttttat aaagtccaaa ccaaagggcg agaatgaaaa gaattccaaa
    61261 ataagttata ggaccgtgga aggaaagtac tggtccaaga attggccaag cacctactac
    61321 cggaagatca atgggattta aagttgaggg aatcgagtag ttttgtcggt agtaaaaaga
    61381 agtgatgcct gcagccaaga tattcaagat tactccagca ataatctggt ccattttcca
    61441 gttcactgcc attagggcta gcagaagccc catcattgct ccagtgaaaa ctccaacaaa
    61501 cattccccga acggtatctt gcaaaatagc acccgcaaaa aaggcagtaa aagctgaaat
    61561 caacatggtt ccttcaatac caatattaac cactccagtt cgttcgcaca taacgccgac
    61621 catcgcggcc agtgctaatg gaattgaaaa ttgaattgcg gtatatagga cagaagtggc
    61681 aactaatcca tctctgctat aaaagtaagt gaaggcaact agaaatgtga gtattacata
    61741 gtgactagtg cgcagtttca ttagttgctc catccactag tcatggcgac attctcttta
    61801 cgattcttaa agaactttgc cagtagcggt gcagtgacaa aaaatagaat cattgcaaga
    61861 agaagatcaa ctagttctgg cgccgcgccc gcttcgaatt gaaccgtaga ggcactcgcc
    61921 ctcatcccac caatcagaat tgccgcaggg attacgccaa gcggattggc tctaccaagt
    61981 aaagctacgg taataccatc aaatccaaga cctgcgttaa aagctggttc aaatctccca
    62041 gtgatactta aagtttcaac agcaccagcg attcctgcaa ccgccccacc aatcaacata
    62101 cttaatatga cagttttctt gatagagata ccggcatacc agccggcatt cttatttctt
    62161 ccaatagttt caatgctaaa accagttgta gtatttttca tgatccacca aagcgttatg
    62221 gacagagcga tagccagaaa gaatcccaac ggaaggccca gcaagtcagg cattcgtgct
    62281 gaatcttgta ttttcggcgt tctagtcaat ggctggtctg gttctttaaa tggcgtgctg
    62341 gtcaagtaat cagcgagagc aatgacaatg ctattaagca ttattgttgt aataacttca
    62401 tgaactccac gataggcctt tagtattgcg gcaactagag caacaattgc accgaagaaa
    62461 gcaccaaata tcagtgctag cggaatatgt actaaagcgg gaagaccgac aaatgtgtag
    62521 cccgcccaag cagatgcaag cgctcctgaa atcaattgtc cttgtgcacc aatgttaaac
    62581 agtccagccc gtaaacaaat tgctactgct aagccactca agattaaagg cgtagcttta
    62641 gcaagcgtta ttgcgattcc atcaagtgaa ccaaatgcag ttgtgaataa aactttatag
    62701 gcaagcaagg gatcaatccc ttgaataaaa accaggatgc caccgaaaat caatgcgacc
    62761 agaattgaaa gaattggaac caggacaggg cttaggtcaa gcttcttctc ttgcttagct
    62821 tccacaagtt gctccaatga ttttttaaat ctttaacttc ttattgaggt aaagggagat
    62881 acgctcgtta aaaagcgtat ctcccttttt tacaacctta acttaagtct tagagcttca
    62941 ctccggtctt aacggttccg ttaagaacct ttggagtaat tgccttcaac ttagcttgat
    63001 atgcagcagg aactttcttt gcgaagtcat gcagtggagc gagtccaaca gctccaaagt
    63061 agtttccgcc cttatctgtt ccttcaacaa tggtcttgat aacagcagca acacccttat
    63121 caattaactt cattgctgaa gttacaaggc aaggacgtgc ttcaggaaga gttccccatt
    63181 gatcgctatc aacgccgatg caatatgcac ctttcttctt tgcaacgcga agtagtgcac
    63241 cgtttccagt gtttccacct gcgccaaaga tgacgtcata accctgggag agaagctgct
    63301 gcgcagttgt tccaccccat gctgggtcgt tgaacgcgtt atctggagcg tgataaatca
    63361 attttgttgc tggatacttc ttgccgagtt ccttagcggc atatgcaacg ccgttacggt
    63421 atccctctcc aaagtaacga actggtggaa caacttcagt tccaagaact gcgccagtct
    63481 taccagtctt tgttagatgt ccagctagat atcctgcaag gaatccagcc ttatcttctg
    63541 ggaaaactag tccagttaga tttggaagcg ctgctccctg gaattgatct acaccaatga
    63601 acttaatttt tggataatca gtagcggcct tagtcatggc ctctgccatg aggaagccaa
    63661 caccgataat tacattgtat ttcttatctg caaactggcc gatatttgtg gcatagtctt
    63721 ttggatctgt tgtttcgatg tatttagcga aaccgccatt tgacttagcc gcagctttgg
    63781 caccttccca tgcagattgg ttaaatgact tgtcatcaac ttttccagta tcagttacta
    63841 aaccgatgca gaaaagtttt ccggtaccgc aatcactttc cgatgctgcc ttagatggtg
    63901 ccgcaaaacc tagagctgta agtgcaacta cagctactac agatatcacg cgcttaatca
    63961 tgaatctcct ctcaaagatc tctggcattg aggtatgccc gagggtaaat cgatcgatta
    64021 ccagtggaga aataagttca tttttatgac tatttcgggg aggctcactg atacagggaa
    64081 tcctatccat gccagaattg aaggtgaaat gctggccgat accaataacc ccgtgttcca
    64141 tcacattttc gttacctatt ttcacctttg accataactc tggttcccaa gtatcaactc
    64201 tggctttaga ctgcttgggt gagaactatc gattttctcg agcagggcga tggagcgata
    64261 actctgattg atcagcgcct gcttccccat gagaggaaaa tcctcacaat aaaaactcac
    64321 gaagaattaa ttgaagcaat ccaaactctg gctgttcgtg gcgccccagc cctgggagtt
    64381 gccggagcgc tgggagtggc tctaatcgcc cttcgcagcc agcaagaaag ctggagccaa
    64441 gagcagagag atagcgcaat tgctgcctta agagctgctc gccctaccgc ggtcaatctt
    64501 gcatggggag ttgatcaagt aactccggaa ttagtgaacg gcccttccgc tgttttgaaa
    64561 tccgctctag ccattagccg cgctgatggc cttgcaaatc gcgctatggg aaaacttggc
    64621 gctgcctatc tattagaaaa gtgtggaacg agaaaattaa ggatccttac ccattgcaat
    64681 actggatcac ttgcaacaag tgattggggg acagctcttg gagtaattcg agagctaagt
    64741 gagcagggat atatcgagga agtatttgct gacgaaactc gccccctgct tcaaggctct
    64801 cgcttaacgg cttgggagtt aagtgaggct ggaattaatt atcgaatcct tccagatgga
    64861 gcggcagcaa gcgctctatt ctcaggactt atcgatgcag tattaattgg agcagatcgc
    64921 atcgcaagaa atggcgatag cgcaaataaa attggatcac ttggggtagc agttgctgct
    64981 aaagagcgtg gcattccatt tctagtagtt gctccagaat ccacaattga tagatcactt
    65041 gctagtggag ctgagataga gattgaattt agggcagata gcgaggtaac ccactttgct
    65101 ggaaaagccg tcgcaccagt tggggcaaag agttataacc ccgcatttga tgtgacttta
    65161 gctagatata tcactgcaat agttactgaa aaatgcgtct ataacctgct agctggggat
    65221 aaattatgaa cgcaccatgt cattatggct ttgagataaa ggccggagga taacgatggt
    65281 ctcagagatt aattcactta attcagagag tgaaattgcc tctcttatca aaatcggaaa
    65341 ggcagtagtt gataggggat tagttgttgg ctctggaggt aatctctcca tgttattaaa
    65401 aggtggcaaa gaatttttaa ttacaaaaac tggtagtgaa cttgatcaat tagatcagag
    65461 cagctttgct ctaatggatc ttgatggaaa tattgctaag ggatcagcaa agccctcatc
    65521 tgagtatcgc gtacatctag agagctatcg aactagaaag gacatcaata cctgtattca
    65581 tttgcatcct caagcaagta tctttgcagc agcaattggt gaggatatcc agcttgtgac
    65641 tactgatcat ttatattacc tgcgaaaaat tatccgcatt ccttggattg cgcctgggac
    65701 tcaagaggtg gcagaggcaa ctgcaactgc ccttaaagat tgcaacgttg tcattcttga
    65761 aaaccatggc tgtgtagtgg ttgccgataa tcctcgcctt gcttactcgc gagccttaaa
    65821 cctcgaagag gctgctgaaa tgacaattcg tagcgcctta ttgaaagtta agccaaagtt
    65881 agtcccgcaa gaatttgagc agtaccttaa gcagcagggg ctatgagtag gagatgcaga
    65941 tatgaaaatt gattgggagc tattaagaag agaggcaatt agcgcttcaa agaaggctta
    66001 tgccccctac tcaaactttc cagtcggagc cgctggctta gttagtgatg ggcgaattgt
    66061 tagcggctgc aatatagaaa atgctagcta tggcttaacg ctctgcgctg aatgttccat
    66121 gatctccaat ttgctgatga gcggcggcgg aaaactgatt gctgtggtct gtgttgatgg
    66181 caagggagaa cttctctcgc catgtggtcg ctgtcgccaa cttttatttg agcatggtgg
    66241 aaatgaactt caactcttaa cgccatccgg atcgcaaaac atgtcggtga ttcttccttg
    66301 gggttttggc cctgatgatc tagaaaaaaa gaattaaata atgagtgaag cctttgatgc
    66361 tgttgagatt atttcagcaa agcgtgatcg aaacgagtta agcgatctgc agattgactg
    66421 gattattgat gcctataccc gcggagtggt agcagatgaa caagtctcag ccttattgat
    66481 ggcgatttta ttaaatggaa tgaatatgcg agaaatttcg cgctggactg atgcaatgat
    66541 taatagcggt gagagaatga attggtccag tcttggaatt ccaacagttg ataagcattc
    66601 aacaggtggc gttggagata aaattacact tccacttgcg ccactagttg ctgcctgtgg
    66661 tgctgctgtt ccacaactat caggtagagg tcttggccat actggtggaa cgctcgataa
    66721 aatggaatca attccaggct ggcaagcatc tctaagtaat gatcagatgc tcaaagtatt
    66781 acaagatgta ggagcggtaa tttgcgctgc tggtgcaggt ttagcgccgg ccgataagaa
    66841 gctttatgcg ctgcgagatg tcactgggac agtgccagcc attccactta ttgcttcatc
    66901 aattatgagc aaaaagattg ctgaaggaac tagcgcccta gttctcgatg ttaaaaccgg
    66961 gtctggtgca tttatgtcag atccagagaa agccaaagaa ttagcaagtg ttatggtcga
    67021 acttggaaaa agagcgggag taaataccag agcccttgtt actgcaatgg atgtgccact
    67081 tggactcacc gccggaaacg cacttgaagt tcgtgaatca atagaagttc tttcaggcgg
    67141 cggaccagca gatgttgttg agttaacttt aattttggca agagagatgc ttgatgctgc
    67201 aaaggtaagg ggcaaagatc ctgaaattgc tctcaaagat ggaagcgcga tggatcgctg
    67261 gagaaaaatg gttaaagctc aaggtggaga tcctgatgcc ccgcttccca ctgcgaagga
    67321 gaagttggtt atcactgcag agaaaagcgg aactatctct tcaatggatg cgcttaaagt
    67381 tggaattagc gcatggcgcc tgggagctgg aagagcgcga caaggggagg cagttcaagc
    67441 tggagcagga attgaaattc atgccaaacc aggagagaag ataactagtg gatctgctct
    67501 attcacactt catagtgatg atagcgagag atttgagcga gctaagcaag cacttgaggg
    67561 cgcggttagt atttctgaga agggtgaagt tgcagagaga ttgccgcttg taattactaa
    67621 gttctaaata ccaattggat gccagagcgt cttatattcc ataaaggaaa gaatccgctc
    67681 aggagatttt tcagctgaaa aactatgaac tctctttaaa ttatcaacac ttgaaatgcg
    67741 aagagctgca gcgcttttgc tatcaacacc gcttaaatca aggccatcta tctccatatg
    67801 cgaggctaat tgcggggtta attcatcact tagaccggtg aggatattta ctacccctgc
    67861 cgggacatca gatgttgcaa ggcattcagc aagagttatg gcagaaagtg ggcgcttctg
    67921 acttgcaagt aagacggcgc aatttccagc tgccagtact ggagcaagtc ctaatacaac
    67981 acctagtagt gaagactttt catcggcgac aattccaacc acgccaagtg gttctgcaac
    68041 tgtgaagtta aagaaagatc ctgcaacagg attagtactt cctgcaattg ttgaaatctt
    68101 atcgctccat ccggcatacc agacccaggt atcaattgct tgccttacct gcaattgagc
    68161 ttgcttacta ctaacagatt ccattgccat aatctctgca acaaattgct cgcttctacc
    68221 ttccataatt tcagcgatgc gatagagaat ttgtcctctg ttataggcag tggcagaggc
    68281 ccaagatgaa tgagcattct tagctgcaag aactgcatcc cttaaatctt ttcttgaggc
    68341 ttgagcagga tttgctatga agtttccttt tttatctcta atctcatagc tacgccctga
    68401 ttcacttcgg ggaaaagcgc ctgcaataaa taatttgtaa gttttcttga tatcaagact
    68461 catcacttag ctcccttgag atatgcgcca agtccatggc gtccaccttc gcggccccag
    68521 ccagattctt tatatccacc aaatgggctt gctggatcaa atttattaaa ggtattggcc
    68581 cagatgactc cagcctttaa gtgatttcct gcccagagaa ttcttgaacc tttatcagtc
    68641 caaactccag ctgagagtcc aaatggagtg ttgttggctt tctctatcgc ctcttgtgga
    68701 gttctaaaag taagcacaga tagcactggt ccaaagattt cttctctagc aatgcgatgc
    68761 gcttgcgtta cgcctgtaaa tatcgttgga gcaaaccaga atcctttatt tggaatctgg
    68821 caaggagcag accagatatc agcgccttct gcttgtccag aagatgagag ttcatggatt
    68881 ttagctagtt gtgcagctga gttaatggct ccaatatctg tgttcttatc caacggatca
    68941 ccgaggcgaa tcttggacat tctcttctgt aatttattta agaattcatc atggatattt
    69001 tcttgaagca gaaggcgaga tcctgcgcag caaacatgtc cttgattaaa gaatattcca
    69061 ttgatgattc cctcaactgc ttggtcaatt ggcgcatctt caaagacgat atttgcgcct
    69121 tttccaccaa gttcaagagt gaccggggtc ttgctaacgg ctagagatct agcaattagc
    69181 ttgccaactt cagtagatcc agtaaatgca accttatcaa tatctggatg cttaactaac
    69241 gcagctcccg tgcttccatc tccggtaata atgtttacga ctccaggtgg gagatctgct
    69301 tgttggcata cttcagcaaa tagcagagcg gttagtggtg tagtttcagc aggctttaac
    69361 acaactgtat taccagtagc tagtgctggc gcaatcttcc aggcgagcat tagaagtggg
    69421 aagttccaag gaattatctg agctactact ccatgagctt ttggtttatc tccaaagcca
    69481 gcaaactcca acttatctgc ccatccagcg tgatagaaaa agtgtgcggc agagagtgga
    69541 atatcgatat ctctagtttc tctaatcggc tttccgttat ctagggtttc aaccacagca
    69601 aattctcgag atctctcttg tagtagacga gcgatgcgaa agagatattt tgctcgctct
    69661 ttgccgctca gcttcgacca aacttttaca taggcagatc ttgcagcctt aaccgcctta
    69721 tcaatatcta cttcatcggc aagtgaaatc ttggccaata cctcttcatt agcaggattt
    69781 atcgtttcaa agcgcttctt gttatgagag gcaacgaatt taccgtttat atatagccca
    69841 taatcctttt tgatattggc tatcgcctta gattctgggg caggggcgta ttcaaagtca
    69901 ctcattatta atccaccgta acataatttg cgctcgaata tctgcctgta cttaatttca
    69961 tgcgttgcat aagtaaatca tttaatagcg cgcttgctcc aattcgaaat agctctgggt
    70021 ttagccactc cgctcctgca atttctttaa caagcactaa ctgcttgatg gcatccttag
    70081 ttgtccttat tccaccagct ggtttaacgc caattctgat ctgacttagc tcaaagaaat
    70141 ctcttaccgc ctcaagcatc actagaacta ctggagcggt agcagctggc gtaacttttc
    70201 cagttgaggt tttaataaag tctgcacctg caagcattgc caagtaagaa gctttgcgaa
    70261 cgttatctag agtgactagt tcacctgttt caagaattac ttttaagtgt gctttatctg
    70321 aacagatttc tttaatagct ctgatttcat caaatacttc accaagttgg ccagagagaa
    70381 atgctccgcg atcaatgacc atatcgatct cagttgcgcc aagggaaata gcatctttag
    70441 tgtcttgcag cttaactttt cttgaagctc tacctgaagg aaaggcggtt gctactgcag
    70501 ctactggaat actttgaccg ccaattgaat ctagatgttt tctagcaatt ccaaccatgt
    70561 cgttatagac gcagaccgct cctacatgcg gaaccgaagc atcttgtgga tcggggcgaa
    70621 gagctttcga gcaaagtgat ctgattttgc cagcagtatc tgcgccttca agagttgtta
    70681 aatcaatcat gctaattgcc atatcgatgg cccatttttt gctggtggtt ttaatgcttc
    70741 tagttccaag cattgcagcg cgctcttgag cgccgatttg atctacgccg ggaagggttt
    70801 gtagaaatct tcgcagcgaa gtctcagaac catcaatcag atttagcgct gacataagta
    70861 gtgagtctac aagttatgaa gcaagaaggg tacgtagctc taaatccagc cctttgatga
    70921 tctcctcggc cttttcctga ttgtcgctaa ctacctctat gtaacactta atctttggct
    70981 cggttccact aggtcttacg ataatgcgaa tgtttggctc aagaaatatt cgcacgccat
    71041 tagttggtgg caggccactt agtggcttct ctaaatcatc aaagccaatc acgcgaaatc
    71101 cagcaattga atgtggagtg ttgtttctaa accttccgag aattgtggtg atttgttcaa
    71161 cgctattcac tcttactgaa atttgtttgg tgccgtgata gccatacttg ctccagattt
    71221 catcaaggaa gtcatttagc gttctagatt cacgctttag atctgttgct atttgaacaa
    71281 tcattagtgc tgcgctgatt ccatccttat catttactga atttgaatca actgcataac
    71341 caagtgcctc ctcataacca aagtgcaggt tatctatttt tgcaatccac ttaaatccgg
    71401 tgagtgtttc agcaaaaggc agttggtaag ccttagcaat tttgcttaag attgaagatg
    71461 agacaattga gttagcaagt gtggcgtttt tatccttagc agctctggct aaatattcac
    71521 caagaatggc tccgacttca tctcctctaa gcattctcca gttgccacgt cgatctgcaa
    71581 ttgcaacggc gcaacgatca gcatcaggat cattggcaat aactagatct gccgatactt
    71641 gcattgcttt cttaatagag agatcaatag ctcctggctc ttcaggattt gggaatttaa
    71701 ctgttggaaa atctggatct ggttctgcct gctccataac caatatcggt ggagcgaaat
    71761 ttgctgctgc aaatacctgc tctaaagtct ttgtgccaac gccatgcatg gctgaataga
    71821 ccaccttgag atcactattt gaggtggcaa gctttgctgt ggccttgata taggaatcaa
    71881 ctattgtgtt atccaaaact tctgctccgc tacttcgttt taacgtgttt agatcgggaa
    71941 cttgagagat atattctgaa atctgcgtat cggcaggaga gataatctgt gacccttcat
    72001 agcgaatctg atcaactatt ccgcctaaat agaccttata gccattatct tctggtgggt
    72061 tatgacttgc agtgaccata attccaacgt cacactttaa ctctctaaca gcataggcaa
    72121 gcactggagt tggcagagct cttggcaata aatagacctt cataccagcg ccttgcatga
    72181 tctcagctga atcttgcgca aaatcttttg atccatatct agcatctcgt ccaatcacaa
    72241 cagatgtgag attgtgtttg cgcatgtaag taacaaggcc tgctgcagtt tttgttacca
    72301 ctgctcggtt cattcttgat ggtcctggtc ctaatttgcc acgcaagcct gctgttccaa
    72361 attctagaaa accagagaag caggagcgca gctcaacttc attcctatct gcaaggagtc
    72421 tttcaagttc tgctcttgtc tttgggtcag gatcttggga aatccactgc tgaacttgct
    72481 ctattagaga ttgatccata gggccaactt actaccgagt acttctatta cttaaatctt
    72541 ttatgtaatc ctcaggagct cctgcttcgc gcgcagcgtt aaccataatt tctagataac
    72601 gagctgatgg cattccacct tcaaaagaat taagtacata gatataacag agcggagaac
    72661 catctagagt ttctactcgc actctaattt ttcgatataa atcggtagtc acaccctccc
    72721 attgatcaag ggctgcttca tctgttgcag ttagatcata gatagagaca aaaacgctat
    72781 ggccagcatc ttctgcaaga gtggcaactg ctccctccca gccaatttcc tccccgccaa
    72841 aagttaagcg ccaaccgcgt agccacccag ttccgcggtg tggtgaataa ggggctctga
    72901 gaagcatctg cctaggatca agatttgatc catatgcggc atataaagtc atgacaaatc
    72961 aaaccattga taacctaatt tgttaatggc gctgcgcaat aatggcaggg aaaggccaat
    73021 cacattagat gtttcaccct caatccttga gataaaaggt gagcttcttc catctagagt
    73081 aaagccccca gcaacattta gaggttcacc agttgctaca tagccatcaa tttcagcatc
    73141 gctcatatct gcaaagaaaa cttttgtact caccacatca ctaatttcaa tctctttctc
    73201 agtgtcaatg atgcaatgac cagtgtgaag taggccagat tttcctcgta attgtttagc
    73261 tcttgcaact gcattttccg cacttcctgg tttacctaaa ctctgtccct ggaattcaaa
    73321 ggtagagtca caaccaatta ttagcgcagg gtagttaatt tttgctccca ctgtatgtgc
    73381 tttaactatt gccagggcaa ttactagttc tttaggggat aggttttgat agtcagagct
    73441 ctcttcatca actccactta caattacttc aggtgtaatc ccagcgcttt gtaataattt
    73501 atatcgagca ggagatgctg atgccaagat tatttttggc gccattattt aagcccttcc
    73561 tagagcccta aatttccaac cagccttgac ccagagatct ctatctagag cgtttcgacc
    73621 atcaataatg attgcttgat tgacttggtt ttttatggca gatggattga tctctcgata
    73681 gatcttccat tcagttaaat gcaaaatcac atctgcccct gcaacacatc cttcaatact
    73741 ttctgaaaaa gttaaatgag aaaagcgcgc tcgagcaggc tcaatagcct tgggatcgtg
    73801 aacagtcaca tctgctcctg cttggcttag ttgaactgcg atatcaagtg cgggtgagtc
    73861 tctaacatca tcactatcgg gcttaaaggc agcacccaat actgcaatct tcttaccctt
    73921 tagatcttgc cccaggtcca ctcgcaccaa atcaattact cgcgctcttg ctcgcagatt
    73981 aatcgagtca atttctctca agaactcaag ggattgttta gctccaagtt cctctgatcg
    74041 cgccataaat gcacggatat ctttaggcaa acagccacca ccaaagccaa ttccagcttg
    74101 gagaaagcgg gagccaattc ttgggtcata accaattgcc ttagcaagaa ctgttacatc
    74161 tccaccagat gcttcacaga tctcagccat tgcattaatg aaggagattt tggtagcaag
    74221 aaatgagtta gctgcaactt ttacaagttc tgcagttggt agatctgccc ttatccaagg
    74281 aactccatct ttaagaagtg aggcatagac ctcttttaat atttcctctg cgcgatctga
    74341 agtaactcca acaactaatc tatttggcct tagggtatct tcaacagcaa aaccctctct
    74401 taaaaattct ggattccagg ctaactcaac cttctcattt acttttctta acatctctgc
    74461 aaggcgacct gcagttccaa ctggaactgt ggatttgcca acaattagag atccagcttt
    74521 ggcatggggc gcaacagatg cgatagatgc atcaacatag gtgagatctg cagctaggcc
    74581 atcttttttt tgaggagttc ctacgcagat gaagtgaacg tcgcaatctc ttatttgcga
    74641 aaaatctgat gtgaacttta atctctgtga attaacttct gccagaagaa gttcctcaag
    74701 acctggttca taaaaaggaa ctttgccttg attaagaagc tcgattttct tctggtctat
    74761 atcaactcca acaacttcaa agccaagaga tgacatggct gcggcatggg ttgcgccaag
    74821 atagccagtt ccgattactg agagctttaa cataactgcc aaatactagc cgctacatgg
    74881 attatcagcg gcggttccac tcttaaattt gttaatggtt gtaggtgatg aggatggatc
    74941 aggccttacc tcttcaatga gggcttcatc atttttaatt ctttcaaaga gttctggagc
    75001 taagactgga tcccagagca ctgcagcgct tactccattt ttattgtagt tgtaatactt
    75061 caatggaata gtaagcgttc taacgttact tgctgaaaga ttacgtagtt gttttcctag
    75121 agttaataag tcgccttgac ttaacccttc atctgtaaca acactatcta gcgcagagtt
    75181 tatgaaatca accatcttta ccgggtttag taatactcca gcgctagttg ccttacgcag
    75241 catagcccca gcaaattcct gttgtctctt cattcttcct agatcaccta aaccatcaaa
    75301 gactctagtt ctaacaaact ttaatgagtc gacaccatct agaacatgtg ttcctgcagg
    75361 aagggttaga tggcttttag gatcattaat atctttcttt gtgcaaatct caacgcctcc
    75421 cagggcatcg accatacgaa cgaagccaac aaaattaagt tcgacgtaat gatcaattct
    75481 taaatcagac atactttcaa gagtttcaat taatagtggc gcgccacccc agttataagc
    75541 agagttaatc tttgaataag cagcaggaat tactttgcct tgagaattat tatgttctgg
    75601 gattagcgca taactatcac gaggaataga gatgattgcc gctttatctc gcttctttga
    75661 gatatgtata agtagcatgg tgtcgcttct cttgcctgcc gcaacatcag tgccgccaac
    75721 ttttaaccgc ttgatctctt ctcggctcaa tccttctcga gtgtcagaac caacaattaa
    75781 gtaattaacc gccgaagatt ctttctttgg acgattctct aggcctgcaa aggcatcaac
    75841 tcttggaatc gctgctgtga tgcgccccag acctgcccag gagatagccg aaatccccac
    75901 aatagctatc gaggctatgg tgaagaaacg aattgcccga ggggatttca cagaggcaag
    75961 agtaatgaag cgatagcgtt tgaacgttat gactagccca cttgagcaag gtaatactgc
    76021 tcacctagcc aattacccac ctgtttccat aattctcaca gtaatcaatg aagcacttca
    76081 tctgcgagct gcaataaaag ccgctttgtc ttcaaactat caaggcgaac tagaagttat
    76141 tttggcagta gggccttcaa cagatgagac atggcaaatt gcaaaagagt tagcagaaca
    76201 agattcaaga attacagtcc ttgaaaatat ttcaggaaga actccaagtg gtttaaatgc
    76261 tgctctcaag ctggcaaaga atcaaatagt tgttcgcatt gatgggcact cagaaattga
    76321 taaggaatat attagaaaag ctgttgagac tttgaaagaa actggagccg taaatgtagg
    76381 cggaattatg tttgctcaag gaactacctc atttgaaaaa gcagtggcag tagcaatgcg
    76441 gtcacaaatt ggggttggat cttcaagatt tcatgtcggg ggagaggctg gaccaacaga
    76501 tactgtgtat ctaggagtat ttcaaaagag tgcattagat gctgttggtg gttatgacga
    76561 gagatttacc agggcacaag attgggagtt aaattatcgc ttgagaaaaa atggtggcgt
    76621 agtttatttc gaccctgagc taaaagttat ctatcgccca agaagttcac ttaagaaact
    76681 tgctttgcaa tactttgaat atggaagatg gagaagagca gtagtgcgtc aacatagtcg
    76741 gactatcaat tttcgctatc tagctccacc tttaaacttg atattgcaag ttgtttcttt
    76801 acttctagcc ctctttgctt catcattatt tttaatacca atcatttctt atttaggcgc
    76861 aattactttg gcttcattaa ttattggaaa aggtctagcc cagaggattt acttgccaat
    76921 tgttttgatt ttcatgcatt tttgctgggg atttggtttc attacctcac caagaaagct
    76981 aattaccaac taatttccta acgctgcggt accctaagaa atatgaagaa aagagcgttt
    77041 ctccttactt ccctatcgct tatcttctct ttagggctaa acgtaatttc tcctgttcat
    77101 gcgcggacgc taatacctac tgattttcgt ttctttggct caggttatgg tcatggtgta
    77161 ggaatgagtc agattggtgc gcgcggacag gcgcttgaat caaagactgc tgttgaaatt
    77221 cttaactatt actacccagg cactgcagta actgcttatc cagataatca actaataaga
    77281 gtaaatatcg ctaatttagt ttcatcggta acgcttaatg cagttggatc tattggcgag
    77341 atacgtctat accaaggtga tatcccgtta agtgagagcc ccgagccatt tggaatatat
    77401 ccaggtgata caactgctct ctttacaaac tttgcaggaa gtgtagttcc agctctctca
    77461 tctcctactg ctaaatatgc tgcgattaat cctgcacctg cttggacagt tcggtgggat
    77521 agcgcaacta caactgcgct tctaaccaat ggtgcaactg ctactcaata taagtatggt
    77581 caaatagtct ttaagagcgt taccaatctt cttagttcat atctagcagt aactaatacc
    77641 ttaagacttc atgatgagta tctctacggt ctaggtgaag tgccttcgtc ttggccagct
    77701 gctgcccttg aagcacaagc aatagcggcg agaacttatg ctctaggcaa attatctcgg
    77761 cttagagttg agtgcgattg caatatttac aacaccactg tggatcagaa ttttgtgggc
    77821 tatgccaaag aaactgaggc aatctatgga attaaatggc gtgaggccgt tgatcgaacc
    77881 tttgtcgatg agaataacgc tttagttgta acccttgagg gcaaaccaat tcaagctttt
    77941 tacttttcat caagtggggg agttactcaa aatgttgttg atgtttgggg aagtccactt
    78001 ccttacctaa ctggagttcc tgatccatgg agcttagatc caacaattaa tcgcagatat
    78061 gcactttggt caaggtttgt gccacagagc gttatggcgc aagcctttct gctacctaat
    78121 gttgtgtcat ttaccattaa ctcaagaact caaactggaa gcattagttc aattacagca
    78181 atttcaagta ctggggcaac cgcaacgcta aatggagaac tctttcgagc tagagtaaaa
    78241 cttccatcaa cctggattca taacacaaag gcaattgtga agctgccctt tatcgctaaa
    78301 gagtgtctac cagatattgt tgaacgatct aactactgcc ttatttaatt agagtgcagc
    78361 acgctattta agccgcccca agttccactt ttattaagtg cttcaagtgc gccgctctgg
    78421 gaatttctac ggatcaaaag tgaattagcg ccagatagct ctcttgcttt gactactttg
    78481 ccatctggta gaagtacttt tgagcctgca gttaaataga cgccagcttc taaaacgcaa
    78541 tcatcaccaa gggaaattcc aagtcctgaa ttggccccta gaagtgttcg cttgccaata
    78601 gagattactt cttttccgcc gccacttagc gttcccataa tcgaagctcc gccgccgata
    78661 tctgaaccat cgccgacaac aactcctgcc gagattcggc cctcaaccat tgatgcgcca
    78721 agagttccag cattgaagtt aacaaagcct tcatgcatca ccgttgttcc aggtgataga
    78781 taagctccaa gtcttacgcg atctgcatcc gcaattcgca ctccctctgg aacaacataa
    78841 tcaagcatcc ttggaaattt atctacgccg taaattgcaa gatagccata tttagcacgc
    78901 agctttaaac gcacctgatc aaatccagcg atagcgcagg gcccatggga agtccaaaca
    78961 acattgttaa gaatagagaa aattccttca agtgataacc catgtggttt aactaaacga
    79021 tgagagagta ggtgtaatcg caagtaggca tcagcaacac ttgctggcgg cttgtccagc
    79081 tcaatttctg tctttactag ggctcgagtt atctctcgct cgttatctgc cccttcaagg
    79141 cttgttaagt caactctgct ttcttcagct gcgcccaggc ctagttgatg aaaataggta
    79201 tcaagtatcg atccatcttt tgcaatggtt gaaatcccat aaccatatgc cgcgctagct
    79261 ttgctcaccg tccaacgcta ccaactattg gctagcactt gcccagagta agcgtaagaa
    79321 aaaaactgct ttagagtgtg gctatgaaag ttgccgttta ttgcgcatca tcgaccttag
    79381 tttcaaaaga gaatctagag cttggctttg aattaggggc atcccttgcc aaattaggcc
    79441 atgaattggt ctggggaggc ggggcgatat cggtaatggg tgaggtggcc aagggcgcaa
    79501 gagaaaatgg cggtataact attggagtta ttccagagcg gctaatgagt gtggaattta
    79561 ttgaccatga tgcaagccag atgcatgtag taaaagatat gcgtgagcga aaaggtttta
    79621 ttgaaaactt agccgatggt tttatcgcac ttcctggtgg gattggcaca ctagaggagt
    79681 tattggaaat ctgggtggga agatatttag gttttcacaa caagccaatt gcaattctag
    79741 atccaagtgg agtctatggc tcgcttcgat tagcgctaga tgacttaact gcaatgaact
    79801 ttatgaaacc aggtcagcat gagttagttt cttggtgcaa cacaattgac agcgcgataa
    79861 agcatgtgac ttcatgagca tgaatagtaa gtccaaactt gccgacttta gagccagcgt
    79921 aactatctct gcgccaattg gtgaagtttg gaaaaagttg gttgattgga agagtcaagg
    79981 tcagtggatg gctctgacaa aggtgaattc aagtgcggat aaagatggaa gctcaggtat
    80041 tggcacaaca atttctgcat ttactggaat tggaaaaatt ggcatctggg atgagatgaa
    80101 aattactgct tggagcccag agactttttg cgccgttgat cactatggtc gttggattaa
    80161 agggattgga gaatttcgtc taatagagat agcgccaggt gaaactagat ttgattggta
    80221 tgaacgaatc gatgctccag ctgcgattct ggccctgata aaaccaggaa tcttgcttgc
    80281 tgtgaattac tccttggcga agtttgcgcg aggatttaac aactgaaggg gcgccatcgg
    80341 cagtagcctt agccttgtga gtaagcagaa ccagacatta gaagagttca tcgactctct
    80401 tcctgaagaa gaacggatcg tgcttaccct ttattacgta aagagatata gcatcgagga
    80461 aatttccgag aaaatagggg ctcccaagcg tgcaatcacg gcggtattga gctctggacg
    80521 gatgagaatg agctcggctt tcaatttccc acaaggtagc tagataaggg ataattccct
    80581 ctcccagctt ggagctgggg cttaaggcta aaagatcttt caaggattga tttagagaga
    80641 aggctaatcg atggcagcca tgaaaccccg cactggcgat ggacctatgg aagttaccaa
    80701 ggaagcccgg agcctagtga tgcgaatccc gctcgagggt ggcggccgct tggtagtaga
    80761 gctaaatcaa gaagaggctc gaaatcttgc cgacgtatta aatgcgtcaa tctcccttct
    80821 taagaagtga acctaccgat tccgaaactc ggaacggtaa aacttccggg ggaaatctcg
    80881 gagaaattct ttaaagattt ttctcatatc gctcttccaa taaaagcaaa agaatcaaag
    80941 gaagaacctg agatagagat tattttaagc aaaaaaatca tacagagcat tgaaaagttt
    81001 tattcaattt cacttaaaga tgagattaag aaaaatggcg gaactgttac tggtaaagct
    81061 ggagaagtaa ttgatattcc agtactgcaa gcaagtggag atgtagttcg cattctcctt
    81121 gttggagttg gagattcaac taattcccac attcgcagag catcagcagc tattggtcga
    81181 aagataaagg ctagtaactc ctcggttctc tccttcttgg ttgaaaagga aagtgatgct
    81241 caacttcact ttatctcctc tgcccttgct acttatattt ggactcagaa aagtggcaca
    81301 aaaagcctaa ctccagaaat tactcttgta ggagattttg ctgaagtgct agagcgcgca
    81361 aaggtgttag tgaagtcaac ctggaaagct agagatctaa tacagactcc ctccaatatt
    81421 aaaaacccag cttggatggc tgctcaagca aaatcccttg caaagagccc cacgctctct
    81481 ctaaagatta aatctggtcg ccaattggca gagtttggcg ggcttagcgc gataggtaat
    81541 tccagtgtta agaacccacc aagatttgtg gagttaagtt ataagccaaa aggatcaaag
    81601 agggcgccac atgtggtctt agttggcaag ggaattacct ttgatactgg aggagtttct
    81661 ctcaagcgcc cctatgacgt aatgactgca atgaaaagcg atatggctgg cgcagcttgc
    81721 gtactaatgg caacaattgc tgcggcagaa ttaaagttaa atgttcgaat cacagcgcta
    81781 ctaatgcttg ctgagaactc actttcaggc acatcaactc gtccatctga tgttattact
    81841 cattatggtg gaactacagt tgaggtaatt aacactgatg ctgaaggaag attagttcta
    81901 gcagatggtc ttgcttatgc tgatatgaat cttgatccag attatttaat tgatgttgcg
    81961 actctaacgg gagctgccac ccttggtctt agccgatatt acggagcgat gtataccaga
    82021 gacaataaac ttgccagaaa gcttgccgac attggcgatt acactggaga tcaaatctgg
    82081 catatgccat tagttgatga ttattcaatt gccctagaga gcaacattgc cgactttaat
    82141 cacaccgctg ataagtttaa gtttcaaggt ggctcagtta ctgctgcgct atttttggaa
    82201 aattttgttg gcaagcgcaa gtgggttcat cttgatattg ctggtcctgc cagatccgaa
    82261 gttgatgcag gagaaaatgt taaaggtgga acaggatatg gagttcgcct actgacccag
    82321 tggttagctt cgctgtgatg tcaggcattg gagaaaaagg atttacggag aagttgtttc
    82381 ccgaacgcgg agatatgcgg gcattttcaa acacctttat tccagagaca gagataattc
    82441 aaaaagctag agctagaggg caagaaattg gtacctacga tgcaactcca gcagttggat
    82501 cgctacttcg ctatctcacc catttgatag gagctagctc gatagttgaa attggaacag
    82561 gggctggggt tagtggttta tggatcttcc aagcgctgag ttcaaagggt ctattaacat
    82621 caattgatga cgaagtggaa aatgctaaat tagcaaagca agcctttgat gaagcgggga
    82681 ttaattcatc tcgatatcga ttaattactg gaaactctcg tgaaattgta ggaaagctgg
    82741 ccgatagcct ctatgacata tttattatga gaaagagcgg agatttactt gatggaattg
    82801 agaatgcaca ccgctcactt cgcccaggcg gagtattagt cattgataga gcacttctct
    82861 ctggaaaggt tgcagatcca acacagcgag atgaagatag cgtggcctat cgagaagtta
    82921 ttaaggtgat taaggactca gcaaccctct ggcttccaat gttgctgcca gttggcgatg
    82981 gcgtaatggt tgcgacgaag atttaattaa agaataggat aggtgcatgt tcggcattgg
    83041 aatgggcgag tttctaggtt tagtgatcct aggattattt ctagttggcc cagataaatt
    83101 gccggaggcg gcaaaagact ttgccagatt tattcacaag gttcgtaact tcacaaccta
    83161 tgcttcgcgt gaattaaaag aaaatctagg acctggattc gaagatcttc aagttacgga
    83221 cttaacgcct aaaaaattag cccagaaagt attaggaaat gccttagatg atgttaagcc
    83281 acttgcaaat gaccttaaga gtcaagctaa agcaagtaaa aaagttattg atgaggctgc
    83341 caaaatagat ccagatctac tatgagtttt tcaaaagatg aagtagtaag tcaaattcat
    83401 agcgcactag caactgtctc tgaccctgag cttcacagac ctcttccaga tctaggaatg
    83461 gttgaaagtg ttgattttga tagtgggcta gcaaaaatca agattctgtt aactatctca
    83521 ggatgtccta tgcgagataa attaaaaagt gatgttagta gcgcggttac taaagttgct
    83581 ggggttgaaa aggttgaact tgaatttggc gtaatgaatg agactcagag agataatgtt
    83641 aaaaaacttc tccgcggcgg tcgggagaag ttcattccat ttgctcaacc tgattcctta
    83701 accagagtct gggctatctc atctggcaag ggaggggtag gaaagtcatc agtaactgta
    83761 aatcttgcgg cagcgcttag taagcgcggc tttaaagtgg gagttctaga tgctgatgtc
    83821 tatgggcact caattccaag gcttttgggc attgaagggc agaggccaac agctattgat
    83881 caaacattta ttccagttga aaccaatgga atcaaggtag tttcaattga gatgttcaaa
    83941 ccagatcgcg cagatccggt tgcttatcgc ggcccactgc ttcatagagt tttagaacag
    84001 cttttaagtg atgcatactg gggagatcta gacttcttac tccttgatct gccaccagga
    84061 accggagata ttgcaatttc tcttggacag ctaattccag catcggaaat tattgtggtg
    84121 acaactccgc agattgcagc tgctgaagtt gctgagcgag caggacgtat tgctcatcag
    84181 ctaaaacaac atgtcatagg agtaattgag aacatgtctg cttatacaga tccggtaagt
    84241 ggagctcagg ttgcactctt tggacaaggt ggagggcagg aaactgcaaa aagattaagc
    84301 caacttgtag gagtcgatgt tccgctactt gctcagattt catttgatat caagctacgt
    84361 gaaggcggag atgaagggtc accgattgtt tttagggatc cagaatcaga tgcggctaaa
    84421 ggcttttttg caattattga tcaactcaca aagcgaaaga agtctttact tggcgttccc
    84481 cttggggtcg ctacctaatt tctcatcaag catttctttg atgtcggcca acagttcttg
    84541 acgcatagct gcaatctctc tgaccatgaa ttcagtaaca gccaagttac gctcatcacg
    84601 ctcacgatct tcttgagatg taacacggtc tctatcagct tgtctattct gagcaagcag
    84661 aatcagtgga gcagcgtaag aggcttgtag tgaaagcata aatgtcagga agataaaagg
    84721 atattcatca aagcgcaggt tttctggcgc aacaaagttc cagatgatcc aggttgcgac
    84781 aaagactgtc atataaacca agaaacgtgc ggtgccgata aaacgagcaa aggcctctga
    84841 tacgcgacca attacttcag gatcataatt aggacgcaag gagcgacgag actctcttgg
    84901 agtttcaagt tttgacttac cagccattgt catttttctg cctcacttct gcgccaattt
    84961 gaaggcaata ggtgatcaag tacatcatca acagttacag cgccaagtaa gcgatcatgt
    85021 tcatcaacta cgggagctgc aagtaagttg tagttagcaa gatggcgaac cacatcattt
    85081 agaggtgtat ctgcagaaat agcatttgtc tctttctccg caactatcgc cagggataac
    85141 gcaggaggtt cacgcagtaa gcgctgcatg tggactactc cgattagtct tccagtaggc
    85201 gtttcaagtg gtgcacgaca gacaaaaacc tgtgcagcag atgcagcaga aacgtctttc
    85261 tgtctaagga ctgctagtgc ttgggccaca gttgcatcac cagcaagaat tactggatct
    85321 gtagtcatca tgccacctgc agagaaatcc tcgtatgcca taaggcggcg aacatcttgg
    85381 gcttcttcat cttccataag ctttaagaga ctctctgctt tttcactacc aacttctttg
    85441 agaacatcgg cagcatcatc aggattcatc tcacttaaaa catcggcaac tagctcgcct
    85501 tctaaaagtg agactaaggc aaggcgatct gcatcatcca tctcctctaa aacgtctgct
    85561 aatttttcat catctaatgt tcgagcaatt tcactctgac gatttacttc aagttcgcga
    85621 agagccgcgg ctatgtcttg cgcaggaagt agagctatat cttcagttgg cgcgcttaaa
    85681 tctggaatgt gagaagaaac ttctatttca acgctgctcc aagaaagtgt ttgagttgaa
    85741 cgacgcccaa aagatctagt tttttcttga acatgtacat gctcggcata ccaatctttg
    85801 ttatcgcgca actccatacc aatatcttca ataatgacga cattaccatt ttctatcagg
    85861 cgtgttttat gatcaagtaa ctcactaagt agtgcaattt caccatgcct aggttgatag
    85921 cggcgaatat ttaaagagcc agtaataaag actccaccgt cattaattga ggtcactcga
    85981 gtaattggaa tgaaaatttt acgtcgctgg gggacttcga cgatgaaacc taaaattcta
    86041 ggagctaggt tgtcctgtcg cagtgatgca atggcgtcgc gtatttttcc tacgcgatcg
    86101 gcatttggat caaatacggg agtaccagcg agcttggcta aaaatactcg agttaattcc
    86161 tctgttcgca tcaggtacct ccatagtcca aggctatcgg atagtaagtt gagccggtgc
    86221 gcgatcaaaa ccatacccag atgcctagcg gtcgtgacat tccattattg cttcttggag
    86281 taataggaat tggaacctca ggtccagtaa ttgccttgag tgcgatgcca attattactc
    86341 tgattttctg gcgaaacctt ggcggagcgc taatcatggc gccctttgct ttgagaaatg
    86401 gtcagtggtt aaagagtgaa aatcgtcgag gtcttgcctg gtcaatggca gcaggatttg
    86461 ttctagctct tcactttatt ggatttttca tagcaatgag atacaccact gtggcggcag
    86521 gaactgccct taccgctctt caaccaatat ttgctgccta ctttgtgaaa aaacttggcg
    86581 gccatattcc aaggcgagct tggatcggaa tggttattag cttcattgga gttttaatta
    86641 ttactggagt tgattttcag atttcaacta gagctttcat tggagatata gcagcaatca
    86701 tctgcgctgc tcttgctgcg ctatatgttt tattgggctc aaaagcacaa caaacgcttg
    86761 caacttcgac ctataccact ttatgttact taacttgttc gctaacagca cttccgattg
    86821 cgctaattag tggagtgcag ttaattggtt ttgaggccaa gcaatggtgg ttattgcttg
    86881 cattaatttt aggagcacag attcttggcc acaccatgtt taatttctca cttaagagag
    86941 tatcgccagc aattgtttcc ttaattgttt tctttgaagt tccagttggc gctcttcttg
    87001 ccttctggtg gctagatcaa ttacctccac ttggaactgt tccaggattg attctcttat
    87061 tggccgggtg tgcgatattt gttatgcgta gcaaggatga agaaaaaggg tagccttacc
    87121 ctcatgctta ttgatttaca tacccacacc aatgcaagtg atggtactga tacgccatct
    87181 gggttaatcg ataaggcaat tgctgcaggt attggggtat tagcgctaac cgatcatgac
    87241 actactcgag gctggcagga agcatctaag gcgctgctta atcatccctc tggttctgat
    87301 atggaattgg ttttaggttc tgaaatttct tgccaagatg aagatgggtt aagcattcac
    87361 atgctcggtc tgctatttga tgcaagccat caagggctaa ttaatgaact tgataagaca
    87421 agagagcatc gtctcttccg catggagaaa ataattactc gtctaaatga agctggaatt
    87481 gaaataagca ttgatgaggt ctatgccatg agaaccggtg atgccacact cggccgccct
    87541 catctagctg atgctttagt tgctaagggg catgtggcat ctcgccaaga agcatttgaa
    87601 attttcttac ataataaatc taagtactac gtaaatcact attcaccaac accagaggct
    87661 gcaataaagc taattaagca ggctggaggt gttgccgtaa ttgctcatcc atttgcttca
    87721 aaacgtggca agactcttaa agtagaagca tttgataatt taattgctgc cggactcgat
    87781 ggaattgaag tcgatcaccc agatcactca gagagtgaga aatccgagct agtctcactt
    87841 gcaatcgcgc gaaaccttgt tattactggc tctagtgatt atcacggcga tggcaaacta
    87901 aaccgattgg cgcaattcac cacaaatcct gaacaatggg cccatcttga atcaaaagca
    87961 aatcagagaa gggttgtgaa gcgatgagtg atatcagcac aattacattt ggtctgcaag
    88021 catttgtaac cctacttgtg attttagatc cgcctggtgc tacgccaata ttcttatctg
    88081 ttctaggaga tcgcactcgc cggcaaagaa ctcttcttgc ctggcaagct gctggcactt
    88141 cactatttgt tattacttta tttgctctct tcggccaatt tattgttgat tatctagaga
    88201 tttctatgcc agcgctaaga ggggctgggg gattacttct tctattggtc tcacttgatc
    88261 tactaaaggg aagcgacttt agtggtggcg gaaagattcc aaagaacttg gctttagtgc
    88321 cacttggaac accactacta gcaggtcctg gcgcaattgt gacaatcatg ctctttagcc
    88381 aagaagtttc caacacttct atgactcttg cactttcact tgcggtacta ggagcgcact
    88441 taataattgg agttactttg atgttttcac tcggcattgt gaaaatcata aaagaatctg
    88501 gagtggcact ccttgcaaaa attgcaggtc ttttaactgc ggcaattgca tttgagatgc
    88561 taatgactgg aatcaaggat ctctttgcct catgagttta ggtttctttt cccccgattc
    88621 cataacctgg aaagtccact cagatccttc aatgtttgtg ggaggaataa gagcgctgct
    88681 tgagcaagcg ctgcatcctg aggcgatggc aggggtggca gctcactcta acttccgtga
    88741 agatgcatgg ggaaggcttg agagaactgg agattatgtt gcaactctta cctttgctag
    88801 caaggaaaaa gccgaaaagt tagctgcaag agtcagagga gttcatgaga agttaaaact
    88861 tgatgatcaa aggcttctgc tctgggttca tatggcaatg gttgattctt ttctagatac
    88921 agcgcttcgc tcaggacttg ttttgagcga aaaagagcgt gatcaatatc tagaggagat
    88981 ggtggtattt gctcgcttag taggtattga tgaagaaaaa gtaccaagaa acgttgctca
    89041 actagataaa tacttcatag atattaaaga tcagctttct gccagcgatg acgccaaacg
    89101 agcagcgtta tttatcgccc ttcctccact tcctccactt cttcgttttg gaactccaat
    89161 tgcgccgcta tggggaggca ttacttccat tgcagcagct tctcttccta agtgggctaa
    89221 atctttatat ggctggccaa cacttcctgg ccaagatgtt gccaccaata ttgctcttag
    89281 atcgcttcga agcgctcttc tcttagtccc tgaaagttta agacaaactc ctgaaatgaa
    89341 gtttgcattt gctcaagttg gtgcagagaa gtgaaggttc ttgttgttgc taatagcgct
    89401 ggtggtgttg gtaagagcac atctgttcac tgtatcgcgg tagcggctag tgagtatggc
    89461 aagaaagttt tagtaataga tgcagatcca tcagcggggc taacttttgc ttgcggtatt
    89521 gaaaatccta gagtgagtac aaaagagttc ttatctggcc agttctcact tgatgcctca
    89581 acaataaaaa gcggagagcg cttttcaatc attgcctcat ctagtcgtct gttagctcta
    89641 gattttgatc aagttatctc acaggcaaaa ctaactgagc ttctagaaag ctttgatcta
    89701 gtaataattg atacgccaac tggagtttct agaattacta cctactttat ggctgtcgca
    89761 aatctaattc tgctacccag cacaactgaa attctctcgc ttcggggagc gctaaatagc
    89821 aaagattatg ccgagagcgc tggccatagc gggcaacttc aacttttaat aactaaaaca
    89881 acacaagagc ttgatgagga gtttaagtca ctgattggcg atgatttttc actccttgaa
    89941 ccaacaattc gctcatcggc gcaagtctgc ctatcgcaga gtcagtccaa aagcatctta
    90001 actctagata caaagagtga agttgctgca gattatcgag aagttactta cttactcttg
    90061 gaaaaactag aacttattta atttaagctc cggcaaatcc aacacggcgt tcagcaatat
    90121 cgccaatctc aattgaaccg atttttcctg caggcaccac gaactgccga cctctaatgt
    90181 ccgttagcac tagcggggca gaagaggtca gggctttatt gaccagcgct aatacttcat
    90241 cgcgatcaag agaagtctct atcgttaatt cacgaagctg atcagctaca gagatacgaa
    90301 cttctgattt tgcagattct gattttccgc tcttttttgt actcatagcc ccatcctaaa
    90361 gagttttagt agattttgct tattcaaatt ttctaaactc tcctaacttt gtagcgggaa
    90421 gccagaaatt cctcgccaga gtagattagt aactagcgag gttgcttcgg agcgattaat
    90481 ttttccatcc cgctcaagcc agtggcgggc agttatttgg gcgcaaccaa tcagcccaat
    90541 tgccagcacc atagattcct cttttggaaa accagtatct agtgagataa cagcgctaac
    90601 cgctactgcg cagtcatagg tcatgcgatc aagcctctgt cttacttgag gttctgcgct
    90661 catatcgctt tcaaatagca gacgaaatgc gccaccttca tcatcaataa actcaaaata
    90721 agcaccaacg gttgccttga ctcgattctt attctcttta tttgaggcaa tagctttttg
    90781 aatcgcaaaa accagagaat cgatatgaac atcaagaagg gcaagataaa gatctagctt
    90841 tgaaggaaaa tgttgataga gaactggctt actaactccg gcccgatctg caatttcatc
    90901 catcgctgct gcgtgatatc ccgaaacggt aaagacctca agggcggcgt ttaggagcag
    90961 agcgcgtcgc tcatcgcgtg gaagacgaac ccgattatca actttctcgc tattgctcat
    91021 cggtgtcatg gtacgaggtt tggcaagatt tcactaatag aactcgtgta gccttcctaa
    91081 cctctatgag aaaaccacgc ttacttctag ttcacgccca tccagatgat gagactataa
    91141 acaatggtgt aacgatggct aagtatgctg ccatgggtta tggcgtaact cttgttacct
    91201 gcaccagagg tgaagagggt gagattttag ttccagaact cgaacatctt gcaagtggaa
    91261 aagaagatgc tcttggtcca attcgcgaag ttgaattagc aaatgcaatg aaagttttag
    91321 gagtggtaga tcatcgcttc cttggttctc caagtaaatc atggcgtgat agcgggatga
    91381 tgggatcaga gccaaattca aggaaagata atttttggaa cgctgacgtt gatcaagccg
    91441 cggaatcttt agttgaggtc attcttgaga ttaaacctga tgtaatgatt acctatgatg
    91501 aaatcggtgg ctatggacac cccgatcata ttcaagccca tagagttgcg atgcgagcaa
    91561 gtgaattggc agctcagcgc ggctggcaga ttcaaaagat ttattggaac acaattccga
    91621 tatcaattat tgaacaggga attgaagcaa tgaaaggcag cggaagtgat ttctggggag
    91681 tggagaaggc agaagatttc ccttttgctc aaccagacaa tctagttaca acggtaattg
    91741 atggccaaga ctttgttgaa aagaaaatgg atgcgatgcg agcccatccc actcaaattg
    91801 cagtagatgg accatttttt gcactctcag ataatttagg ctttaaagtt tggggacaag
    91861 agtattaccg cttggtttat ggaaaagcta gcgcaccatt taatgaagag ggacgcgaga
    91921 ccgatctatt ctcaggaatt cagctatgaa gtctttaatt agtttgctct ttggagcctt
    91981 agttggagtc tctggaacat ttctgcataa tgcatataga ccttttgggt taatagtttc
    92041 attactagcg ctatttattg gacttcgatt agttagaaat atgtatctct ctaagttatc
    92101 actcgcactc tttgcctttg gttggctatt tgtaattgtt agagcttcaa gtcttggtaa
    92161 tggcggcgaa gttctaattg aagcaaatgc ttatggaaat ctttttgtat ttgggggagc
    92221 agcgcttatt tcctggcgcc tacttaagag gatttaagtt ctaactcctc caactccagc
    92281 tctggcccga tggtgaatcg tttatggcaa ttccaagtaa gagaagttga tctcgcattt
    92341 gatcacttaa agcaaaatcc ttgttttctc gcgctctctg tcttgcctct aacaagactc
    92401 tggcttcatc cggaagctct ttaatcggca tctcttccag tagttttaga gcaagaagtg
    92461 gttcaagatt ttctaaaatc gctgccttct ggccatctga aaattgatca tccttttcaa
    92521 gggatctgag cttgatcatc acttggggag tatctaaatc tgcctggaag tgagcaagaa
    92581 ttgccagtac aaatgcgcta gcttcatctg aataatccct aggcgctaat ttccattgtt
    92641 gatatttagc tctcaagcgc tgcaatgttg agtgcgctgc gcttattgag ttccaggata
    92701 aatccatctg agagcgatag cggttttcaa gaaagcaaaa acgcaatgaa agtggatcaa
    92761 acccacgcgt gattacatca gagagcagaa ccacatttcc agaactcttt gacatctttc
    92821 tcccttcaaa taagagatgc tcgccatgaa gccaggcact aactgcttca cctttgacta
    92881 gaggatttga ttgggctctc tcgttttcat gatgggggaa tctaagatca atgccaccaa
    92941 tatgtagatc gacatgtcct tgcagataat gaagcgacat tgcagaacat tcaatatgcc
    93001 agccaggaaa tcctggacca taagctgtct gccagatcat ctctttacga ttaccggccg
    93061 ctttccaaag ggcccaatcg gcatgaaatc tctttgctcc agcttcaaca tattcatagc
    93121 gatgaccagg cttgagcgca tctagtcgat ttccacttaa ctggccataa ctatcaaatt
    93181 tagtactatc gaaataaatt gaggaatcat caccttgata ggcaagttca agttcagtta
    93241 atttcgcaat atgttcaagc atcatttcga tgcactcact tgctctggga tagttatgag
    93301 ctgggagaat gtttagcctt gctaagtctt tatgaaactt ctcttcatac tctcttgcaa
    93361 tagagtatgg atctcgcttt tcaagtttag attgggccaa cattttatct tcttcaaaat
    93421 cttcactcat atggccaaca tcggtaatgt tttgtaccat cttgactttc aaaccagaga
    93481 actccagagt cctgcggact aaatcactaa gcaaaaaagt ccttagattg ccaacgtgag
    93541 cgtcgcgata aacagttggt ccgcagcagt agattctcac cacttggcca tcacttgcag
    93601 taataggcag cacttttcgc tctaaagtgt catagatatg tagcccatta tttggttttg
    93661 gctcgcgctt caccccctta ttttgtagga ttaggccctg caatgtaagc atcgcttatg
    93721 ccaattcggt gtttatatct tttagatttt ctctgaaagg agtctcaggt gacatacatc
    93781 atcgcccttc cctgtgtaga tgtaatggat aaatcatgta ttgaagagtg ccccgttgat
    93841 tgcatctatg agggcgatcg aatgatgtat atccaacctg atgagtgtgt ggattgtggc
    93901 gcatgtgaac cagtttgccc cgttgaggcg atttattatg aagatgatgt tccagcgcaa
    93961 tacagtgaat ataaggaagt taatgcagaa ttctttgacg aattaggctc acctggtggg
    94021 gcagcaaaag ttggaagagt tggcaaagat catccagtcc ttctgcagat tcagaaaaaa
    94081 cctgcaggcg agtaagtagg cgaagataaa actccctgat tttccttggg atgccctagc
    94141 gccatatggg caaaaagctc gagcccatag ccagggaatc attgatctct cccagggaac
    94201 tccagttgat cccaccccag atttcattca agaagaactt aaatcaagca gtgattcacc
    94261 aagctatcca ttaaccactg gaagtgcagc ccttagagag gcgctcaaga aatattgcgt
    94321 tgaaacactc ggcgcaagtg gcgactttga tgtgctgcca acaattggtt caaaggaatt
    94381 agttgccctt ttggcatatc tactggaggc taaatcagtt ttaattccag aaattgccta
    94441 cccgacttat aaagtcggag cgctactggc tagagcagaa gtccttgaag ttggaattga
    94501 tgccaattct tggcccaaga aagctgattt agcttggatt aattcaccat caaatccaac
    94561 aggtcgggtg catagcaggg aagaactcga ggcctgcctc acctgggcaa gaaaatctgg
    94621 tgcaactctt gcaagcgatg agtgttatct gccctttacc aatggaatta aagctcaatc
    94681 tattttggca atcgcaaatg gtgataacac ctcagtgctg gcggttcact cgctctcaaa
    94741 gcgatcaaat atggcgggct atagaggggc atttgttgct ggagatccta aattgatagc
    94801 gcaattactg gagattagaa aacatatggg gatgatgctt ccgctgccga ttcaaaagtc
    94861 aatggccatc gcgctttcag atcagagcca tgttgataag caggcaggaa gatatcaagc
    94921 aaggcgagag gtgcttgcta aagcgctaat agcggcagga tttaaaattg agttttcaga
    94981 agctgggtta tacatctggt gtagcagaga tgaaaaagat tgggatagcg tgaattggtt
    95041 tgcagagata ggaattcttg ttacgccagg aagtttctat ggaagtaaag gcgatcgatg
    95101 tgtgcgcatc gccctaactg ctagcgatga gaatatttca agtgcagcct ctcgtatcat
    95161 cgcggcagta aatcgatgat aaataaggct attttgctcg ttggtggcag aggaactcgc
    95221 cttgcagaac ttacaaaaaa tactccaaag ccgatgttaa aagttgcagg agcaccagtt
    95281 acggagcatc aaatcgctag agctcaagca gctggagtca ctgagatagt ccttgcaact
    95341 tcatatctag ctgaggtttt cgagccttac tttggcgatg gctcaaagtt tggcatttca
    95401 attaagtatg caatagagaa gcaaccccta ggaactggcg gagcaatctc aaatgcagca
    95461 aagtttttaa acctagcaga agatgaatct gtctttattt ttaatggtga tgttttaagt
    95521 ggccatgatc taagagcgca ggcgcagttt catgaaagta agggcgctga tgtgacgctg
    95581 cacttagtaa gagttgatga tgcccgtgct tatggctgtg ttccacttaa tcaagatggc
    95641 aaggtattag agtttctgga aaagatggat cagccaaagg ttaataccat taatgctggt
    95701 tgttatattt ttaatagaag cgcaattgcg cagattcccc aagatagcgt cgtaagtgtt
    95761 gagaggcaga cttttccaga acttttaaag agcaagggcg ctctatatgg atatgtcgat
    95821 gactcatatt ggattgacat ggggacccca gcttcatata tcaaagcttc aagagatttg
    95881 atttcaaatt ctgcgatatc acctgcaact aggggagtag ttgatggagt aatgatcgct
    95941 aaagatgcag cggtggatgc cacagcaata atttctgatg gttctgcaat tgaatctggg
    96001 gcagtaattg gagctaactg ccaaattaaa gggtcaatta tttcggcagg ggcgagtatt
    96061 ggtgatggtt cgataatcga taattgctat atcgcaccta atgtttcaat agcaagcaat
    96121 gaaaaaatat ttgagaaaat aatcagtaat tagcccactt ttgccccaaa attaaggaaa
    96181 aaatcgacaa atcggggatt aatttctacc caaaaaaagt ggccatcaac ttgacacatc
    96241 cgaataacaa gcgtgtaatt aggccctagt aggcccacca tcaggcgtgg gctctgtttt
    96301 ttaggggtat aactgatgac tgatgctcgt cgaatagagc agactttcat tcctggtcca
    96361 agtatccaat tgggaaatgg cgagatcgtt gagctatcgt ggcaagagcg agcgctatgt
    96421 gcccaaaccg atccggaagc tttcttccct gaaaaaggtg gttcaacccg tgaggctaag
    96481 cgagtttgtc tctcatgtga ggtcagacaa gagtgtcttg aatacgctct ggcacatgat
    96541 gagcgctttg gaatctgggg cggactatca gagcgtgaac gccgtcgcct taagcgccgt
    96601 ccagcttaaa tctaataaca ataagaattc caagttatta actagaagag ttaaggaaaa
    96661 ctggtctaac tttttattta aacaagaggc ggtgtaatgc ctactttgga taataacttt
    96721 gtcaccgcaa tcatcgtctc tcacaatgga gctctctggt tacctgaagt tgttgcatct
    96781 cttgccaagc agaagcgggc aatagatcaa ataattgcta tcgatactgg ctctgaagat
    96841 agttctgtaa agttactgcg aggcgctggt attgccacaa tctcgactgc taaagaaact
    96901 ggttttggtg cagcaattaa agaagctctt ggtaattcaa gagttaaacg aggagatgaa
    96961 tcagaaacag agtggctctg gctgattcat gatgattgcg caccagatgc attcgcgcta
    97021 aaagagctct tagcagccct tgatgagcga ccagcagttg caatggctgg gcccaaattg
    97081 cgcggttggc atgatcgaaa ccacttatta gagctaggaa taagtatcgc ctctaatggc
    97141 gctcgctgga ccggacttga atatcgcgaa gccgatcaag gccagcatga tggagtcagt
    97201 gaagttttag ctgttagcac agctggcgca ttgattcgcc gggatgtctt tgaagagtta
    97261 gatggatttg atccagaact ttctttattt agagatgatg ttgattttgg ttggcgcgtg
    97321 catactgcag gccactcagt aatcgcagtt ccaaaggcgg ttgcatatca cgctgaagcc
    97381 gcttctaatg aacgtaggcg aattgatgtc tcagatgctt ttcttcatcg cccgcttctt
    97441 ctagatcgta ggcatgcggc atatgttttg atggccaaca catcttggtg gctcctgccg
    97501 ctaattgccc tacagcttct ctcagcatct ttattccgcg caattggata tctgctagca
    97561 aaacttccag gatatgcctt agatgaaatt gcagctgttg ctctggttat tttgcagccg
    97621 caggacttat ttagagctag agcaaagcgc agaaagaatc gcttagtttc atcaagagtt
    97681 atctcgcgct tcgttccccc tagaggatcg cagctgcaat tagcaattga gcgagctggc
    97741 tctgctattt ctcgcagttg gcgccgctca tctatatatg aagaaagtgg ggttactagc
    97801 tcggccctag atttaaatga tgaagctcta gaaaatgctg atattgattt agttcaggct
    97861 ccgtcaccaa ttagatggct aactcgccga ccaatactta gcgtttcact tcttgttatt
    97921 ttattgacct tgattgcctc ccgtaatcgc tttggagcga tagttggtgg cgctctagca
    97981 gcaacgccta caaaagcctt tgaactattt tcaatgtatt ccgattcttg gcataccgta
    98041 ggccttggct caagcactag cgcgccgcct tgggttgcct acattggatt tggctcatta
    98101 cttactgggg caaactcctc actatttatt actggattat ttctttgcgc agttccacta
    98161 gcgctttggg gttcatatct acttgcaaag aaatttacta atctccactt ccttgctctc
    98221 attgcagctc tgctttatac cttttcgcca accacgttaa gcgctattaa tagcggcaga
    98281 gttggaacct tagtattggt tgtaattgga ccctggttga ttagatcgct atttggactc
    98341 gagcaacttg aaaatctatc ttggcgaaaa actttctgga ttgcgctgtt attgaccttt
    98401 gtctgcgcct tctcaccaat gacatttatg tcaattctac tctggcagat tattttggtg
    98461 atctttgatg taattgcatt caattctaaa aataatgcga tggacaagac agtctttgat
    98521 agaagaaatg cccgtcgtat tgcggtgaca attactcctc taattgttaa cgctccttgg
    98581 tctattgaat ttattcttca tccttcaaga atccttcttg atcccggttt aagcctggct
    98641 ggaggcgaag tcctctcatt gcttctagga aatcctggtg gagttggagc tccaccgatt
    98701 tggataattt caccaatact tcttattgct gtaatagcta tttttgttag taagacagca
    98761 agattaggag aggtggcgct attttttatc gccattgcag cgctacttgg ttcaaggcag
    98821 gtttcaggcc atggcagttt caccccagag caactctggg ttgggtcgct tctggtgatt
    98881 ccaaccttaa ctgcgctact tgcagcagta ataatgattg ataactatct acctagcctt
    98941 agccaatcta atattgatta tcgccatatt cttcttggct ttacctccct gatttctgcg
    99001 ctctccattc ttgcctcagc tacatggtgg ttgggatcgg ctaacagcgc tccagttcaa
    99061 gccaattcaa aatctgcact tcctgccttt ttaagtgcta gcgcccagac tgagggacgt
    99121 tataagactt tagttttgag agcggagtct tcaaaggtga aatatttcat cgctcgagat
    99181 aaagatctgc aactgggaca agccgatgtc ataacaggac ttgctccagt agtaactaag
    99241 gcaataaatg atctaattgc tggatctgga gttagtagca gtaaagtctt tgctgagttt
    99301 ggaattcgat atctcttttt agcaaatccg attaatgaag agctagtaag aactattgat
    99361 ggcgcaggtg gatttactag agctgcctca actgatgaag gaataacctg gaaagttcca
    99421 ggagcgctag gccatatctc atttctatct caagatggaa gctattcagt cctaccaagt
    99481 ggtgaagttg gtgcagcagg aacccttaca tctgctggcg taataataat tactgaaaag
    99541 tatgacaatc gctggaagat gcttcttaat ggaacctatc tacctttaac ccaaactgaa
    99601 aatggggtac caaggtttta tgtttcagaa ccgggagatt tcctaatctt ccatgacggc
    99661 acctcaagaa gagcctgggt ctcgctacaa ctattgacct ttctgacctt aattattctc
    99721 gctcttcctg ctcgccgcag gagaagtcag atgagtgagc aggagttggc atgaaagaga
    99781 cgcgcgccag ctttgcactt ttgattttta tcataattgc ccatattgct cacttactac
    99841 ctcagagctc ttctaattct gttgttactc agtcatatcc agcaacggcg tgtcctgccg
    99901 gagtatcggg agcaaaagca accgcactgc ttgcaactaa aaatattgag ataagaaata
    99961 tcaatcaacc tagtgcagag ctaaagaaga atggctcggg aagctatcca cttaatcgcg
   100021 gagcgatttt ggttgctgga aatcctgcaa ataccaatgt aattatgacc agatctggca
   100081 agtggagtag cgctgctacc tgcactatta gcggcccgat caattggttt gttggtggta
   100141 cagctaatgt cacaagtcaa tcaaagttaa ttatgatcaa tagtggttta agtgatgccc
   100201 taattgatct aaccgcttac tctgaaagtg gggctagtca gagtcttcca gtgacagtta
   100261 aagcctcatc tgagaaagtt gttcgcatcg actcgctctc acctggttct gagcgaatag
   100321 ttttaaaggt tgaaactcga agtggtcgca tcacttcata tcttctagat gagcgagtgc
   100381 gcggcttaag caacattggc ggagactttg ttccagcaat gtcagaggca agtaaagaat
   100441 tagtaattgc aggtcttgcc gtcaagctgg gttctagtag ttcaatcaaa cataccttga
   100501 ggctgatgag cgttggagaa gttgatgcta gcgctagcgt tgaaatcatc tcacctgatg
   100561 gcgtctatgt tccggttgga attggcgaga tatcacttaa tgctcgagaa gttacagata
   100621 ttgatctcag cagcgttgat ttcggtagca aagcatttgc tctaaaagtt agcgcaactg
   100681 aggagatagt tgcatcggtt ctatctgagg ttaagagcgg atcagtttca gactttactt
   100741 ggagcgctcc aagtcagagc tttaataccg tttcatttaa tatttatggt cttgaaccag
   100801 tgattagctt tgtcggggag aaagtcatcc tctccatagt ttggagagat agagggggca
   100861 agagcaatag tgaattgatt cgaggtgatg agatagttaa ttggcgagtc ccaccaaata
   100921 ccaggctctt aacgattagt agtgcaaccc cggttgttgc agcgatgagt tggataagtg
   100981 gtgatggggt tgctcgcctt ggtattgcgc caagtacaaa ccttgagagt gccacaaagc
   101041 caattgctga cattgcagtt attcagcctc gcggctagta aatcccataa aagttggagg
   101101 taagcgacta atctcgcgcc atgtcatcaa ctaatagctc aaggccaaat tctcgcccta
   101161 gcgctgttgg aagacgatgc acgcgatctg gatgtagcgc gcatgcaatt aaaactctta
   101221 cctatatcta ctctgactcc acagcgctga tcggaccact ttcaacatat gttgaaccac
   101281 atgcctatga tctctgtatt gagcactcag ctaaattaac cgtgccgcaa ggctggagcg
   101341 ttattgaaca taatcctgat cgagaaatag ttgaaccttc tccagaagat ttaatcgcaa
   101401 ttgctgatgc aattcgccag agttttgctg aagagatagc gccaaaggca gtaactcctg
   101461 aagttggtcg tcgtggccat cttcgcgcaa ttccagataa taaataagac cgctaagatt
   101521 taagagtgga tcaagagctg cttgataaag taattaaggc ctatgacatt cgtggcctgg
   101581 ttgatgaaga gataagcgct gacttcacct ttgcactagg aatcgccttt gctaaattta
   101641 ttgaacgaga gcgcgagcca gcaaccattg ttgtgggcga agatatgcgc ccatcatctc
   101701 ctgatttagc taccgcattt tcagatggca taacctcaca aggacttgat gtaattcgaa
   101761 tcggactttg ctctactgat caactctatt ttgcatcagg agctctcaat atgccgggag
   101821 caatgtttac tgctagccat aatcctgcgc aatacaatgg aataaagcta tgtagaagcg
   101881 gggccaaacc aattggccaa gagtcagggc tggcttggat taagtcggca atagctcaag
   101941 gatttcccat tccaaatcgc ccaatgggaa aagagcggag ccaagattta ctagccgatt
   102001 acgttaagtt tctattctca ctctttccag ataagtcttt tgcgccagat tttcaatcaa
   102061 gaccgctgcg gattgcaatt gatgcgggaa atgggatggg agggcatacc gcgcctgcag
   102121 ttatgcgaga gattaatgca atcgttgatg gcatctattt tgaattagat ggaagttttc
   102181 caaatcatga ggcaaatccg atcgagccta aaaatttagt tgatctacaa aatctagtaa
   102241 aggagaaagg ctcagatatt ggtttggcat ttgatggcga tgctgatcgc tgtttcttag
   102301 ttgatgaaaa aggaaatctt gtctcaccgt ctgccttaac tgcgctaatt gcaaaacgag
   102361 aacttattaa aaatccaggt gcgtcaatta tctataactt aatttgctcc aaagctgttg
   102421 ctgaagtaat tactgagtca ggtggtagag cgcttcgctc tagagttgga cactcatata
   102481 taaaacagat gatggcagat gaaggcgcag tctttggggg agagcattca ggtcattttt
   102541 attttaaaga cttttggtca gccgactctg gaatgcttgc tgcccttcat gcaatagcag
   102601 cacttcttga aagtcagatt ccgctatctg agttaatgca gagctttaat cgatacagcc
   102661 taagcggtga gataaataca accgttgttg atcaagacaa agcgatagat aagatcaagt
   102721 cctactttaa agattatgag ccaggcttaa cttttgatga gcttgatggc ctcactgtta
   102781 acgctaagaa ttggtggttt aaccttaggt cttcaaatac tgagccgtta ttaaggctaa
   102841 atgttgaggc cgatacccaa gagcagatgg cttccatgcg agatctggtc ttgcagttaa
   102901 taagaaacgg gtaaactacg ccttctaatc agaggcaagc tctaaaccca tcaggagaga
   102961 aattcaagtg aatccaacaa ctatgactac gccaaaacat gacattgcca atgcttcact
   103021 tgctgctgaa ggaaagaagc ggattgaatg ggctgagcgc aatatgccag tacttgctca
   103081 aatcagagag agatttgaaa aagagcagcc atttaaaggc gttcgctttg ctgcctgcat
   103141 gcatgtaaca actgagactg ccaatctaat gagaactcta aaagctggag gggcagagct
   103201 tgcgctatgt gcttctaacc cactctcaac tcaagatgac acagcagcag ctctggttta
   103261 tgaatatgga attagcgtat ttgctagaaa cgctgttgac cgtgatggct attactcaca
   103321 cattaatgct gctcttgata ttgctccaca ccaagtcttc gatgatggat gcgacttagt
   103381 aaacacccta cacaccacaa gaactgaact acttccaaat atcactggag gatgcgagga
   103441 gacaactact ggtgttattc gtcttcagca gatggcaaaa gatggcgcac taactttccc
   103501 aatgatcgct gttaatgaca ccgataccaa gcatatgttc gataatcgct atggcacagg
   103561 tcaatcaact attgatgcaa tctttcgtgc tacaaaccaa ctaattgctg gaaaagtttt
   103621 tgtggtttct ggttttggtt attgcggcaa gggagtagca gagcgcgcta gaggaatggg
   103681 cgccgaagtt gtaattactg agattgatcc aactaaggca cttgatgcac ttatgcaggg
   103741 ctatcgcgtt atgccaatgg cagaggcagc aaaagtagga gatatcttta taactgttac
   103801 cggcaatcgc actgtgattt cagctgagca tttccaatta atgaaagatg gagcaatctt
   103861 ggccaatgct ggtcactttg atattgaaat tgatgtggcc tggcttgaga agaattcaca
   103921 gaagaaccca aaaattcgcc atcaaaccga tgagtaccta ctaggaaatg ggcgaagaat
   103981 ccttctaatt gctgaaggcc gtctagtaaa tctaggagct gcagagggac accctgctgt
   104041 tgtaatggat atgtcatttt ctgatcaagc tctcaccgct gagtggttgg taaagagcgc
   104101 aaagggactt ggcgctggag ttcatatggt tccagttgaa attgataaag aagtagctcg
   104161 tctgaaattg aagtcaatgg gtgcaactat tgatactttg accccaattc agaatgaata
   104221 tctaaactct tggaagcacg gtagctaatg acttcggtaa tggtcgttga tgacgatcaa
   104281 gaccttgccg aaatgttggg aattgttcta aatggcgtag gtattgaagt cgacctagtt
   104341 agtcggggcg atgaagtact tgaagtcttt cgcaataatc cgccagattt agttttacta
   104401 gacatcatgc ttcctggtct tgatggaata gaagtttgta aagagattcg taaacactct
   104461 atgcgggtac caatcgtgat gctcactgca aagagtgata cccatgacat tgttagaggg
   104521 cttgaagcag gcgctgatga ctacatggtc aagccattta agccatcaga gttagtagcg
   104581 cgaattagaa ccaggctacg tagaacaggc ggagagatct ccagcactct tactatcggt
   104641 gatttagcca ttgatattgt ggagcacaca gttactcgag caggaaaaga gattccctta
   104701 acgcgtcttg aatttgatct cttagtatcg ctagcgaaag aaccaggaag agtctttact
   104761 cgagaagctt tacttggtga agtgtgggga tatcgccact caaccgatac ccgtttagtc
   104821 aatgttcata ttcaaagatt gcgcgccaag attgagcatg accctgaaaa tccaacaatc
   104881 attggcacgg ttcgcggcat tggatataag gcaggcgcgc taggtaatag aagttagaga
   104941 ctctgaaaaa agtgaagatt actcaatact ttagaacctc tttatttaga aaagtatttt
   105001 cagtatcaag cattatctcg atagctttaa tctatttcct tggctccaac ctctataacc
   105061 gtatctctga tggaattatc gaggagaaga tttcggcagc aatttctgaa ggtgaagctg
   105121 caattcaata tgctgactat cgctttttaa ttggtagcct caataaaaat accgattaca
   105181 aaacaattgt cgatgaaatt gttaagtcta caaatgtcag cgcaaaagcc tcgggccgag
   105241 agattgcact tcttagtagc actggcaaaa gggttgctgg cattccctca agatctactt
   105301 caaacttcct tgagccatct tcaattccaa atgagttaag gcagcttgtt aaagaagatg
   105361 atgagattca ttggcagcgc acgaacttgc gatatttcaa tggagagagc gttgctggag
   105421 ttgctgttgg tcatcgtata gatattaagt ctgtaggtcg ctatgaaatg tatgtgctct
   105481 ttgactttgc atcacagcag caaaccattg atctcattgg tcgttcgatg tggggcacag
   105541 gactcattct gatggcattg attatggtta ttgcctctgt agttttaagg caagttatta
   105601 agcctgttag gtatgccgct gaagtagctg agcaactttc cgcaggcgac cttttaaaga
   105661 gaatgcaagt taaaggaagt gacgaagttg ccagacttgg gactgctttc aatgatatgg
   105721 cagatactct tgccaaacag attactcgtc tagagaatct ctcaagactt cagcagagat
   105781 ttgtctctga tgtctcgcat gagctacgaa ctccactaac aactattcgt atggcatctg
   105841 atgtgatttt tgcagctaga gagggatttg acccaactat tgcaaggagt gctgaacttc
   105901 ttctctctca gattgatcgc ttcgaagaat tgttaactga cctattagag gtcagccgct
   105961 ttgatgctca agttgccact ttgtctctgt caaaaattga cctggttcag ctagttaatc
   106021 gctgcgtaga agatttggcg atggatgcta agcaaagaga tgtgaaaatt cgaatcgatg
   106081 tcccagagga tgaagtttta attgatgggg attcgcgacg tattgagcgg attatgcgca
   106141 atttattagg aaatgctatt gatcatgctg aaggaaatcc tgtggactta acagtcaaag
   106201 ctagttcgag cgcagtttca cttgcagtta gagattatgg agttggcatt gccacccatc
   106261 acctggagcg agtctttgat cgtttctggc gcgcagatcc gtcgcgctct cgcattagag
   106321 gtggaacagg ccttggacta tcaattgcta gagaggatgc aaaccttcat ggcggcgata
   106381 tcaagctctg gtcagaacta ggcgctggcg cacaatttgt tttgactctt ccattgacgc
   106441 aaggtcaacc cataactgaa gttcccatag gtgagttacc cacacattga agttctaaac
   106501 acttagttta tttgcctgct ttgctaaaaa tgtctaagtg gactttgata aaagaaaaga
   106561 aggaattttc tcagctctaa cttcattagt ctttccaaaa atttgtattt actgtcaagt
   106621 tgagtttgca ccactttgtt tagaatgtca gcagttctgg cttgcacctg tccagcggcg
   106681 atatgtcaaa ggtctagcag ttaattcagt tcttgcttat aacgcagcaa gttctagaat
   106741 tataattcaa gccaaagaga gtagaaataa agtcgcacaa ttttggatgg caagagcgct
   106801 gagtactgct ttagagaagt ttgaattaga taatccagcg ctaagtgttt cgcattgctt
   106861 actagttccg attccttcaa ctaaaactgc gataagaaaa agaggtggat cgtttctcca
   106921 cccaatcctt gatcaattag ttgcgctgca aaagcaaaag aatggagtgc gctggcgctg
   106981 gaaggaacta cttattcatc agaagagcgt tagagatcag agcgaacttt cctattctgc
   107041 gcgccagagc aatatggcca gagcctttag gcttagaagt gatgatttag taagggaaag
   107101 accaatcttg ctaatagacg atgtattgac cactggggct acgttagata gcgcttttcg
   107161 ggctttaagg gagagaaatc tgacagtttt gggcgctgca acggtatgtg cgtcagccca
   107221 ccgcctacta atacgataga gccctagttc atcaagtgga aggttgatct gtggttgcaa
   107281 ttctcgacaa gattctgcgt gccggagaag ggcgcgcact caagcaatta gaaaagattg
   107341 ctgatcttgt taactcacac gaagcaaatt atgtcgcgat gagcgatgag cagctaaggg
   107401 caatgactga taccttcaaa gcacgccttg cagcaggaga agatttagat gaccttcttc
   107461 cagaagcttt tgctctagtc agagaggcat caaagcgaac actgggacag cgccactatg
   107521 acgttcaaat tatgggaggc gccgcacttc accgcggaaa tattgcagaa atgcgaactg
   107581 gtgaaggaaa gactttggta gcaactctgc cttcatacct aaatgctctc actggtctag
   107641 gggttcacat tgttactact aatgattatc tagcagagcg tgatagcgag tggatgggac
   107701 gtatccatag atttttaggt ttaaaggtcg gggtaattct ggcatccatg agcccggcag
   107761 agcgccgcga acagtacaac tgcgatatta cctatggaac aaataatgaa tttggttttg
   107821 attaccttcg agacaatatg gcttggaact tggccgattg cgtgcagcgt gaacataact
   107881 ttgccatcgt ggatgaagtt gactcaatct tgattgatga agctagaacc cctctgatta
   107941 tcagtggacc tgctgataag gcaacgaagt ggtatcaaga atttgccaat attgcatcgc
   108001 gcttaactcg cggtgagcat tatgaagttg atgaaaagaa gcgtaatact ggaattcttg
   108061 atgctggagt ttcaaaggta gaagagctgc taggtattga aaatctctat gaggcagtaa
   108121 acactccaat gattggctat ctaaataatg ctcttaaggc taaagagcta tttaagcgcg
   108181 ataaagatta tgtgatcatg agtggagagc tcttaattgt tgatgaacat accggccgag
   108241 ttctatctgg ccgtcgctat agcgaaggtc tacatcaagc gctggaagca aaagagcggg
   108301 ttgagatcaa agatgaaaac cagactctgg ccacaataac cttgcagaac tacttccgca
   108361 tgtacgacaa gttagctggt atgaccggaa cagcgatgac tgaggcttca gaatttatgc
   108421 agatttacaa gcttggagta attccaattc caaccaataa agtcatgcaa cgacttgatc
   108481 aatcagatct ggtctataaa actgaggatg ccaaatttga agctgtagct aacgatattg
   108541 cccagcgcca tcggagggga caaccagttt tggttggaac tgttagcgtt gaaaagtcag
   108601 aagttctctc aaacgctctt agacgcaaag gcattccgca tgaagtatta aatgctaagc
   108661 agcatgaacg tgaagcggcc attatcgcaa gagcaggaac ggttggagct gtaacagttt
   108721 caacaaatat ggcaggtcgt ggaactgaca tcatgcttgg tggaaaccca gaatttatgg
   108781 ctgattatga attacaacgt cagggaatct cgccagttga aaaccctgaa gagtatgaag
   108841 cgatgtggcc agctgaaatt gaaagacaga aggctgccgt ctccaaaggc catgatgaag
   108901 ttgttgcact tggtggactt tatgttctag gaactgagcg acatgagtca cgccgtatcg
   108961 ataatcagct ccgtggccga tctggtcgcc aaggagatcc tggtgaatct cgtttctatc
   109021 tctcgcttca agatgaattg atgcgtaggt ttaattcagc acttgttgag cgtttcctaa
   109081 gcgctgcagg cattccggaa gatacgccaa ttgaatcaaa gatggtcacc aatgccatta
   109141 aatctgcgca gactcaagtt gaatcacaga attttgagat gcgtaagaac gttcttaaat
   109201 atgacgatgt gatgaaccgt cagcgcgagg ttatctatcg cgaaagacga gaagttttag
   109261 agggcgctga tatcaaggag caagtcacag gatttattga agatacgatt cgcggttatg
   109321 ttggagcagc aactaatgaa ggatttcctg aagagtggga tctagatcaa ctttggaccg
   109381 ctctgaaagc aacttatccc atctcattta aagttgaaga tattgaagct gaagttggtg
   109441 gcagaactgg tttagatact gattttctta ctgctaaagt cctagatgac atctttgcgg
   109501 cctatgcaaa acgtgagcaa gatctaggtg ctgaagtcct gcgtgaactt gagcgaaaga
   109561 ttctcctttc tgtcctagat agaaagtggc gcgagcatct ctatgagatg gattatctcc
   109621 aggaaggaat tggtcttcgc gcgatggcgc agcgagatcc acttgttgaa tatcaaaaag
   109681 agggatatga gctattttct gcgatgatgg atgcaattaa ggaagagcta gttggattct
   109741 tatttaatgt tgaagtcaag gttgaaaatg aacacgtcga agcaaagggc gttactccag
   109801 ctgctcctaa gcaagagctt cgatactcat cgccaagtga gagtggtcag gcttcaaact
   109861 catcaccggc agtaaatggc ggagtatcta gaaattcacc ttgcccatgt ggttctggaa
   109921 agaaatataa acgctgtcat ggagctgcat agctttctat ataaggctta actcagtaca
   109981 gagccatttg tgatcgaccc cttcaaagcg cgcaacgaga gccctaattc tctctttgaa
   110041 aatcaatgtt gcgtttagtt caacgactcc aactattggc tggcttcttc taacactttt
   110101 aatcttggga acctccttca agcttcctac ttgagtaagt agtgagttat aggtgtagcg
   110161 gtgtgtggtg cgagcaacct gagatgctgg gcgccttgca gccataatct caagagcggt
   110221 aattagataa gtcagagtcc agcgcgtggc attgggaagt tcttcaagag aagagggagt
   110281 gggagcaaaa tctgcttcat agttctcgcc atggctagtt ggaattagat agagcttatt
   110341 gctttcctgg cttacgtctc ttccaaaaag aggagttgaa taaaagctat tttgctcaaa
   110401 tagctctaat gccggatgat ccaataagtc ataatcagaa agatcgaatc ctttacttcc
   110461 tatttctgct tcatttttca tagatagagc atggttatta aatgcgaaat aactatcatc
   110521 taaaaggatg aggagttgtc cacagcttgt taatacctgc gttttagttg tggatgtaga
   110581 agcttcagag agcacgaaat taggtattca ttgccaatga aaaacaccaa taagcttcag
   110641 aaaaattcct ttaacttctc tatccgtagt tatcttaaaa cgattttagg gggatgttta
   110701 attgctgttt catttatctc agctctagtc atctccaatt catcgagccg gatgatcaca
   110761 gtttggagcg ctgcggttga ccttgacaaa ggagaggtaa ttacggcaga agatgtggtt
   110821 atcagcaaag ttttactacc tggtaatgct ggaaactaca tcgatgggca agtctccatc
   110881 attggaagct cagtggttag agccattgga attgacgaat tgataccagc ttatgcacta
   110941 agtagccaag tagatgagca gcttcagcag gttccaattg ctatcgcctt ttctcaagta
   111001 ccaaaagaca ttggcagtgg acaacgagtt gatatctatg gaatcactaa atctgaattg
   111061 cgcactcaaa gtcttgaatc tggtagatat aaaagtcgtt tgattgcaag tgatttggtc
   111121 atagatcaag tggataacca ggcaaatact ctaggagggg acttagtaat aacacttctt
   111181 gtcccaagga caataattcc aaacctgatt gaggatatgg gaaattacga ctttattctg
   111241 gtgggaaatc ggtgaagata aatttaatca ccgcacttag ctctcatcaa gttgaagacc
   111301 aagttattga agttctgcta aaacatgact tccagctcca aaagcgccta ctatcgccat
   111361 cagattttga tttaaagtta atttcaagca tctctgctct tcgtatcttg ataatttctg
   111421 ataaagactt tggattaaat tggcgcgaga taagaagaga aagtgatgag aatctttcaa
   111481 ttcttattct tgatgtggat aagagattta gtagtgatga gatattgcaa caagccaatc
   111541 aggcattaag aggaagtaat gaggctctcc caactggaga ctcaatccgt agagattctt
   111601 gggttttatt tactggctct gatggctccc ctggaatttc aactcttgcg ctaaataccg
   111661 cccaggaata ctccaaatta gcccagatgt tgctcattga tgcagatctt tcccagcaga
   111721 gtctgtctca gatggtgggg gagagagtga gttatcagcg aagcgctctt aataacgctc
   111781 tatcccttca gagcattgct tcttttgatg agattgaatc tagagaagat gagagcgttt
   111841 ttattgatgt cggaagcgcg ccggtaatga gccaggcggt atctgatcga cgcacagaag
   111901 gaaagttttt tatgcaggcc ctgaatagtt gttctcatct aatctacata attcatcaag
   111961 atagccgcgc cctctatcaa ttagaacaat tcgaagagtt tttaaagatg ttctcttcaa
   112021 agctaaatgt gatttattta ctcaacaaag agagtagtag ctccagtcgc ccccttttcc
   112081 gcaaaagctt tagaagcaag atagacggca agcctcactt ctttatgccc tatgagtatg
   112141 gcaatttaga gcgtgctcgc aatcgatatg ccactttgtc tgaagtcaac tctcgcagta
   112201 gcctttctcg agcgctacgg gagttagcca tttatctcca taaaataatc tgaagtactc
   112261 tttcgcctat gccttttcgc catctgctct cagatcagag ttcgctaacc agtgatgatg
   112321 tattgcgtct tggtgaatta actgctgaat ggcaattgct tgcagatatc tcatttgcag
   112381 atttagtgtt atggattcca aagcgcaaag atgaaaagag ttggccggag ggacatcgca
   112441 ctatcgccca aattcgccct atgactgcag caacagtttt tgctcaagac ttaattggca
   112501 atgaggtggc atggggctct agaccagata ttgatcaagc gctatcaagt ggtgagattg
   112561 ttagagatag tagggctgag caagttggag agataaagat aaaggttgag tgcattccag
   112621 tcttttttgc tggtcgagta atagcagtaa tttcacgtca tagaaatcta gagactatga
   112681 gaacgccgtc aaggctggaa ttaaattatc gcgagattgc taaccacatc tatcgaatga
   112741 ttgctgaggg aaactttcca gttaaggaca atgtctattt ggcagaggct gctccaagag
   112801 ttggcgatgg tttggtaaga ctaaatgttg caggagaagt tctctttgct agtccaaatg
   112861 ctcgatctgc attcaatcga attggttggg ataaagacct tgaagggcag aaccttggtc
   112921 gagttttaga ttctcttatt agccaatctt caccgcttaa tccacgggag gagaactggc
   112981 aaataactat gagtggtaag caattgaggc gagatgagtt tgaaaacagt ggtggggtat
   113041 tggatctttt agcaatgcca ttaactgaag gtgataatcg aattggggct gtagttcttg
   113101 ttcacaacgt tacagagcta agaagaaaag atagagcttt aatcagtaaa gatgccacca
   113161 ttagagagat tcatcatcga gtaaagaata atctgcagac tgtttcagct ctgcttcgcc
   113221 tgcaagcaag gcgaattgat gatcctaaag ctagcgctgc tattgaagag gcggttagaa
   113281 gagtggtttc aattgccctc gttcacgaga ctctctctgc taccgcgcag gatttagtgc
   113341 aatttgatga ggttattacc aagataattc aaggtgcatc agagttaaat attaggccaa
   113401 atgagatcca gataaagaag gtcggcgaat tcggccttat ctcttcaatg gttgcaacgc
   113461 ctcttgcctt ggtattaaca gagttgatcc acaatgcttt agagcatggt ttgagtcaaa
   113521 gtggtgattt ggtagagcta agaattgata gaagtggaaa atctatgact gtaacagtgg
   113581 tggataatgg agccggcatt ccaagggact tctcacttga atcaaataca aatcttggtt
   113641 tgcagattgt gcagactcta actaagaatg agttagctgg aaaaatagag tttattaagc
   113701 cagatattgg aactcaggtg cagatttctt ttccgctaga gcgttaaaga attactcctc
   113761 gaagttattt tcagataaac gtgcacgatt tctagcagca cggcgcttca ttgctcgtct
   113821 ttcatcctca gatagcccgc cccagactcc agagtcttgg ccgctttcaa gtgcccattg
   113881 caaacaatct tgtctaacta tgcaaggcat acagactttt ttagcttctt caatttgagc
   113941 aatagcaggc ccagtatttc caacaggaaa gaagagttca gggtcaatat cgcggcaggc
   114001 agcgcggtgg cgccaatcca agctcataag acctcatcaa tcttcaggca aggggcagtt
   114061 cggggaaacc gagattgacc attcttgcgt ctttgggtgt aatgcgaacc agatttgggc
   114121 gcgattactg ccatattatc cccgctaaaa catcttaact caagagtctt gccccgcgat
   114181 ttcacagaaa gttagtgaga tagagcgcta gagctcttaa attaatttga attttgaata
   114241 tttcttagat caagtgcccc cgacaggatt cgaacctgtg cacccgcctc cggagggcgg
   114301 tgctctatcc cctgagctac gggggcgctt tggaaaagat tatcagtgat tcctctgcca
   114361 atattggccc atgaaagaac gagcgtattt tgcaactggt tgcttctggg gagcagagcg
   114421 aagattctgg aatctatctg gagtgatctc aacttcagtt ggatatatgg gtggaaatac
   114481 taaatcacca acatatcaag aggtctgtac tggaaaaaca aatcacgctg aaatggttga
   114541 ggtagttttt gatccagagc aaatttcata tcaaagactt cttgaagagt tctgggtgat
   114601 gcatgatcca acttcactaa ataagcaagg cggagacatt ggaacccagt atcgcagcgc
   114661 aatatttact accacttctg agcagttaga gcttgccaga aaaactcgtc aggtttataa
   114721 cgatgcgctt aagagcgcag gacttggtga aattgttact cagattcaaa gcgcagatgg
   114781 acatgaatat ttcttggcgg aggaatacca ccaaagatat ctagagaaga atcccaatgg
   114841 atatgactgt cactcaagta ctgggattgc atacccagct ctgaaggctt aaggagaaag
   114901 caatgtcata tagcgaatct gaacttccta aaatcaatcc caagactgga aaggcatttc
   114961 cagttcaatt agatgatgaa agtttagcta agagcctgga tccactctcc tatgatgtat
   115021 tgcgaaaagc tggaacagaa gttgcattta ccggaaagta ttatgagagt gaaaccattg
   115081 gcgtttataa gtgcaaagca tgcagcgcag aactctttcg aagtgagact aaattccact
   115141 caggctgcgg ctggccttct ttctatgccc caaaaagtga tgatgcagta gtgctattgg
   115201 aagatcgctc acttgctccg cgaatcagaa cagaagttcg ttgcgcttct tgcggttcac
   115261 accttggtca cgtctttgag ggtgaaggtt atgcagttcc aactgatcaa cgttggtgta
   115321 tcaactcagt ggcgctaacg cttgaaccca aaaactccta gagaatttca gtagcgcgcc
   115381 agcaatacca agctaaatgg ctgcgatatg ggcgatagat ttcacctaat ttaagaagct
   115441 cagctggctt tatctcacct ctcatgcgat gaaccttctc ccatccacgc ctgactccaa
   115501 gatctcccac cggccacaca tcctgtctgc ctagttgaaa catcaaaaac atctcaacag
   115561 tccagactcc aattccaaag agcggcagaa gctgctctct aatttcttca tcatctaggc
   115621 gatgaagaga gctcatagaa acctttgagc taagggcggc ttgagctaac tcctctaaag
   115681 ctctcgcctt tgctgttgag caaccagctt gccttaaatc acttctactt attcgactaa
   115741 tcccactcgg tgaaattcgc cctccagaga ggttcttaac tcttccaata attgttgcgg
   115801 cagctttagt agataattgc tgagagagaa ttgatgaagc aagtgtcgaa aaattacttt
   115861 ccaagggttt ctttgaatta agaccaagag gacaaggtcc ggattgcttg attatgggtt
   115921 taaatctggg gtttttcttt gcgatctccg cggcgatttt tcgcgattgc gccagcgtga
   115981 ttgccatgag aaggcagggt agccattttc agttttacag tctattttta gccgcttaca
   116041 gagttttaca atctcaattt agctctttgc attaagggct ttgaaaggtt ttcatggcag
   116101 ccggtattaa ggaagtcgca actgaggctg gagtctctat cgccaccgtt tcgcgagctc
   116161 ttcgaggttt aaatcatgtt aatcctgaaa ctcgtaagaa aattcttgat gctgcagaga
   116221 aacttggtta tcccatcccg caaagctctg ccaaagcttt atttggaagg actaattcag
   116281 ttggagtagt ggctccattt atctctagat ggtatttcgc tcaagttatt aatggcgcag
   116341 agcaagccct tagagaagca ggtcttgatc tgctgctcta taacttctcg cagatgaaag
   116401 ggcgcgagcg attattccaa catcaattgc taaaaggtcg agtagatgcc ctaattgtta
   116461 ttagccttcc tccaactgag gaagagtttg attcgatgtt aagccttgga attccaatcg
   116521 cacttgttgg aatgcatcat gcacagtgtt ctagcgtggc tattgatgat gttgcagggg
   116581 caaggacagc aactcagcac ttagttaatt taggccataa aaagattgca cttctctccg
   116641 gccggcctga tgacccattt ggttttagtg ttccacaaga tcgccgcaag ggatttatgc
   116701 aggttctagc tgagagtgga ttggagttta agccaactcg tgaagtcttt ggtgacttca
   116761 caatgcatgg tgcaagtaga gcgatggatg atttattggc gagagcagat aggccaacag
   116821 caatattttg ccaatcagat gagatggcac ttggcgcgct acaagcaatt agaagaaatg
   116881 gattaaaggt tcccgatgac atttcaatta ttggatttga tggccatgaa atggcggagt
   116941 tttcagactt aacaactatt gagcagcccg tctctctgat gggagagatg gctgcatggt
   117001 cgattatgga acgattaaag cttcctaaga gcgaatcaca ttcactaact cttccaacta
   117061 ccttggtagt aaggaattcg actaggcgct tggaggcata gaaattaata gccaaaaaga
   117121 attggattgg tggtcaaatt ctgcgattta tcaggtttac ccccgctcct ttcaagatac
   117181 taatggtgat ggtgagggcg atttagttgg cgtaatttcg cgactttctt accttaaatc
   117241 acttggcatc gatgcaatat ggctaagtcc tttttatctc tcaccaaata atgatggtgg
   117301 ctatgacgtt gaagatccaa gagcggttga tccacgattt ggcagcatgg aagatgctgt
   117361 taatttgata aaggcagcac ataagtctga tttaagaatc atcgttgatt tagttccgaa
   117421 tcacttctcg acagagcatc agtggtttaa ggcagctctt caatctctgc ctggatctct
   117481 agagcgttca cgttttcatt tctatgatgg acgaggtgaa agtggtgaga tcccgccaaa
   117541 taattggtgc tcaatctttg gaggtcccgc ctggagcaga gttaaggaag tagatggcaa
   117601 acctggccag tggtatctac atttgtttga ttccacgcaa gctgatttga attgggaaaa
   117661 cgatgaggta aaaaaagatt ttgaaaagac gcttcgtttt tggcttgata agggcgttga
   117721 tggatttcgc atagatgtcg ctcatggatt agttaaggaa gatattttta aagatcatag
   117781 ggatccagaa ggattgacta gagcccttcg ccttgatgtt agtgatatgg atagagttga
   117841 gcgtgaatca cttcttgctg atgttccatt ttttgataaa gagggagttc atgagattta
   117901 tcgcagttgg cgaaaaattc ttgactccta cgaaggccag aagatgagcg ttgccgaggc
   117961 ctgggttcat cccagttcaa gagcgatgcg ttatgtaaga agtgatgaat tgcatcagat
   118021 atttaatttt gactttctaa tcgcgccctg ggattcagaa tttctagtaa gtgcaatcaa
   118081 taaaaccctc tctgaagtcg ggcaagttgg cgcctctcct acttgggtgc tttcaaatca
   118141 tgattctcca agacttgtaa caagactggg tggtgggcaa ttaggaaggc agagagcaag
   118201 agcgatggcg cttttaactc atggccttcc cggtggtatc tatatctatc agggcgaaga
   118261 gctggggctg gaagatggac agcttgaaga taaagatcgc caggatccag tattttttag
   118321 aacgaaaggt gttgataaag gtcgagatgg cgctcgaatt ccaattccat ggagttctca
   118381 attaccaaat tatggattca caactacaaa accttggttg ccaattccaa gtacttggca
   118441 agatcaatgc gttgaagccc aagtagattc cgatagccac ttaacgctct atcgcaagtc
   118501 gcttcatatg agaaaacttt tatcgcgcca gatagcgcaa tcaagtgcga taacttggat
   118561 tgataccccc aagggagttc tagcctttag ccgagggaat gattttttcc tctatgccaa
   118621 caccacaggc cagagcgttg aacttaaact tgagagcaat agccagataa tcctctcctc
   118681 atcggccaag gctcaattaa agtcagacac gctcaccctg cccgctgata gcaccgtttg
   118741 gctggggaaa ataacaaaat agttagttat ttaccgcccg tttacagggg ctaactattg
   118801 cttggtcagg ggccccagag aaggatgggc cctaacttcc tcagagaaat ctgtggaact
   118861 tctatggtcc tttcgaaagg ggaaactata tgaccgcatc attgcggcgt cgtcgacttc
   118921 tgttggcgac ttctttattc gcagcgctat cacttgtatt aagcgcatgc tccagctcag
   118981 atgattctgc tggtggcgca aactgtgatg cctacgaagc gtatcaaggc tatgacggaa
   119041 cagaggtaga aatctattcc tctatccgta gcccagaggc agagatttat caagaatcat
   119101 ttgccgaatt tgaagagtgc acaggcatca caatcaattg gaatggaact gctgaattcg
   119161 aagcacaact tccagttcgt gttgaaggtg gcacagctcc agatcttgca gtcttcccac
   119221 aaccaggtct actaaaggca atggttgcta ctggaaagat gttccctgca actagcgctg
   119281 tttcagctaa tgcagatgaa ttctatggac cagactggaa ggcatatgga accgttgatg
   119341 gaaccttcta tgccgctcca cttggtgcaa acgtaaaatc attcgtctgg tattcaccaa
   119401 agacatttgc tgataacgga tggacaattc caacaacttg gccagagtta ctagctctct
   119461 ctgacaagat cgctgctgaa ggcaaagttc aaccatggtg cgttggcttt ggctcaggcg
   119521 atgcaacagg ttgggttgga actgactgga tggaagacat catgctccgc gttaatgacg
   119581 cagcaacata tgatgcgtgg gttaatcaca ccattccatt taatgaccca aaggttgcag
   119641 cagttcttaa agaagccggt aaggttctaa agaacgctaa gtacgtagga aatgtttcag
   119701 caattgccac aactaccttc caagaaggtg gagctggaat tgttgatgga tcctgcgcaa
   119761 tgcaccgtca ggcttcattt atcggcggaa ttctctctgc tgactacggc gcaaccattg
   119821 ttactccaga agatactgat gttgaaggcg gaattactac cttctacttc ccaggtgtga
   119881 ctgctgatca acgccctgcc cttggtggcg gagagttcgt tggcgcattc tcagatcgcg
   119941 gtgaagttgg agcagttcag ctttatctaa catctcctga atggaataac aagaaggcag
   120001 cacttggtgg ctggttctca gctaacaagg gtctagatac tgcaaacgtt gcagatccag
   120061 taaataagaa tgcggttgaa attctcaaga atgcaaccat cttccgtttt gatggatcag
   120121 atgcaatgcc tgcagctgtt ggtgccggtt cattctggaa agaaatgact gcttgggttg
   120181 cagagaacaa gtccgataag gctgttcttg atgcaatcga gaagtcctgg cctaagtctt
   120241 aaaaagtcct aggcatctca aggagcctaa aagcttctga gaagtagtag ttgtaagggg
   120301 tgtagcactt agaaaagttg ctacacccct tacgcaaagt atcgctcttt gcaataatgc
   120361 ttatggaagt tctaggaaat tcagtaagga atgattactt aaggggagtt gaggcttgat
   120421 gcttgagtta gcagcacgcc aatctcaact tgaacaggct gcctcaaagt taactgggct
   120481 tgtaattacg gtaattgtct ttgcaattgt tgtcagcctt gctttttact tagcagagag
   120541 aacttcagag cgcgttcaga gatatgttaa gtacttcctt tttcttgctc cagcgctaat
   120601 tttcctcttt attggattga tagctcctgc cattagaacg ctctatttat ctttcaggga
   120661 tgcaagagga gatagttttg ttggtttaga aaattacatc tgggccttta cccaacctga
   120721 aattcttcag attttgcgaa atacgggtat ttggatggtt gtcgctccca ttgcttcaac
   120781 tgctcttgga ttaacaattg cagttcttac agatcggatg aaaaggcctg ggttagttaa
   120841 atctttgatc tttatgccaa tggcaatctc atttgttgga gctggaatta tctggaaatt
   120901 tgtctatcaa tttcaaccaa atgaacgagt tgctgaaatt ggcctactta gtcagatagc
   120961 tgagtggtta ggttttgttc caacgaattg gatattaacc gcgcctttaa acaccttctt
   121021 actcatcgtg gtatttgtat ggattcaaac tggctttgcc atggtgattc tctctgccgc
   121081 aatcaaggca attcctgaag agattttgga agcatcaatg cttgatggag caagtggttg
   121141 gaagagattt ggaaaagtta cagttccaat gattcgagga actattatcg tggttctaag
   121201 cactataact atcggggcac ttaaagtctt tgacatcatc agaaccatga ctggtggaaa
   121261 ctttaaaacc agcgttatag ctaatgagat gtataaccag tcttttagag cgcttaatta
   121321 tggtactgga agcgctctgg caatcatttt attccttgga gttattccac tagttgccta
   121381 taacgtgatg catctgcgac gtgagagatc ggagcggtga tggcaaaggc gataaagcaa
   121441 ggcaaagtag aagtagttaa gagtcatcta agctctcctt gggcaagctt tgtagcaatt
   121501 ttggtagcag tgctttggac gattcctacc tttggtctat ttgttacctc cttaaggcct
   121561 cagacaggaa tcaactcatc tgggtggtgg actgtactta ccaagccaga atttactctt
   121621 gagaattaca gcagagtctt atttgaaggt cgcggcgcaa ctcctccttt gagtcaatac
   121681 tttgtaaata cctttgccat tgttatcccg tcaacaatta ttccaataac aattgcgatc
   121741 tttgcagctt atgcaattgc ttggtttgac tttaagggaa gaaactttat tttctttgga
   121801 atctttgctc tccaagtaat tccacttcag atgtctctaa ttcctctgct acagctcttc
   121861 tcaggaggac ttcatattgg aagcgttact gtaattccaa gttttggcat tactggaacc
   121921 tttattccaa tatggctagc tcacactatg tttggacttc cgcttgcgat atttctgctc
   121981 cataacttta ttgcgcaatt gccgagagag ttaatggagg cagcaagggt tgatggcgca
   122041 gattacttca aactatttag gcaaatagtg ctgccactta gcattccagc aattgcctcc
   122101 tttgcaattt tccaattcct atgggtctgg aatgatttgc ttgttgctct tacatttggt
   122161 ggtggcaaac gtgagattgc gcctctaatg gtcagacttg ccgagatgac tggaactaga
   122221 gggggagatt gggagttatt aacagcaggc gctttcgttt cgatattggt gccggtagct
   122281 gttttctttg cgctacagag atattttgtg cgcggattgc tcgctggatc tgttaaggga
   122341 tagcaccttt tatttaattt gattgcagtg gcatactgct acccatggcc gaagttgtta
   122401 gagcccctgg gctaaatgag ctaagaggcc gcaagagcgc taagtggcga ttttttccta
   122461 gcgatgttct gcctctgcca gttgctgaaa tggatttccc gatcgcagaa ccaattaagc
   122521 aagctcttca tgacatggtt aatcgctctg atactggtta cttaggccca atgccagagc
   122581 tatttgaagc attttctaag tttgcagata cacgttgggg atggaaagtt gatcctgcac
   122641 atattcgcac cgcaactgat gctggagttg cagcagttga agtaattcga actctattaa
   122701 agcctggcga gaaactgatg gttaattctc cagtttatga aaatttctgg acctggccca
   122761 aagaagttag cgcaacctta gttgatgttc cactaaagca agagggagag aattactggc
   122821 ttgatttaga tggtattgaa cgagaatata aagctggagt aaaagttcat ctgctctgcc
   122881 atccacacaa tccagtagga gttatctttg aaaaagaagt gctagtaacg cttgcttcat
   122941 tggctaaaaa atatggcgtt gtaattatta gcgatgaaat ccatgcacca cttgtctata
   123001 aaggacgcaa ttttcaaccc tttatggcca ttagtgatga tgcccgcgaa gtatgtgtgg
   123061 ctatctctgg ctctggaaaa gcctttaatc tagctggact caaatgcgct ctaatcatta
   123121 caggatcaga tgagctaaag cagcgaatca acgctatgcc tgaatcggtt ggctatcgag
   123181 cctcaatatt tggagcagtt gcagcaactg ctggctttag tcaatcaatt gattggctag
   123241 atggtgtcat tgcaacactt gaggagaata aagttctaat ccggaatcta attgatagca
   123301 agattccatc gattacctat cgagagcccg actttagcta tttggcctgg cttgatctct
   123361 cagctcttaa cttgggagat gacccagcaa agcacctatt agaaagagga aaagtagctt
   123421 tcaaaggtgg ttatatgtat gggccaaacc ataagcaata tattcgcttt aattttggaa
   123481 ccagccctga gattattact gaagcatttg atcgcatcct caaatctcta tgaagcgcta
   123541 tgacgcaata gtcattggca gtggtcacaa cggcttagtt gcagcttgct atctagcgaa
   123601 agctggaaag agcgttttag ttctggagaa aagcgcatca cttgggggag caacaactag
   123661 tgtgaaagct tttaaaggag ttgatgccaa actctctaga tattcgtatt tagtctcact
   123721 tctgccagat caaatagtta aagatttatc acttgacttt gaaactattg gccgaaaagt
   123781 atcttcctat accccctact ttgatggtga gagtgataag gggctattga ttaattcaga
   123841 gttcgatcaa gaaagtagag attcactctc tgaactaaca gaaggtgatg atgaagcaat
   123901 cgcttgggag agcttctatc aatctattaa agagtttgct gaagttgttg ccccaactat
   123961 gttaaagcca gtgccaacgg agacagagat aaaggagctg gtagatccac ttacctgggt
   124021 agagctagtt gaaaacacct tagctcagag cttaaatgat aatttctttg atgatctagt
   124081 aaagggagta gtccttaccg atggacttat cggcaccttt actagcgctg atgatcacct
   124141 ggctaataag tgctttatct atcatgttat tggaaactca agtgggcagt ggcgagttcc
   124201 caaaggtggc atgggcgttc tagttgatca actgctaatt agggccaagc aattaggcgt
   124261 tgaaatagct accagtcaag aggttacttc aattactcaa ggtgataagc atctattgat
   124321 taatactgta gatgggcaga gctatgaagc aagagttgcg ctagcaaatt gcgcccctaa
   124381 ggttttagag aaagttgctg actttaaggc gccaccactt cgcgatggtt cgcaggtgaa
   124441 gataaatatg gtcttgaaag agcttcctaa gctaaaaagt ggcgttgatc caaaagtagc
   124501 cttcgctgga acatttcatg ttaacgagag ctactttgaa ttagagcagg cttttgctca
   124561 ggcaagcctt ggtcaaattc cagatgtcat tccctctgag ctttactgcc ataccctcac
   124621 tgatccaaca attcttactc cagatttgat agctgctgga tatcagagct tgaccctttt
   124681 tgccctgcat ctgccggcct cactctttga taacgatcat gaccggatta aggctgaagt
   124741 gagtaagaga attttggatg gttttagcca gtatctagaa agaccgattg agagttatct
   124801 agctaaagat agtgacggca atctctgtat tgaggttaag accccacaag agttagaaaa
   124861 agagctagga ctgcccagag gcaatatctt tcatagcgat ctcgaatttc cttggaagca
   124921 ggatggggat agtagaaagt ggggagttga aactaattca gagcgaattt tcatagccgg
   124981 cgccggcgcg cttcgcggcg gtggggttag tggaattgct ggacataacg ctgctatggc
   125041 tgcgttggaa actttagcaa ggctgccata gaattaggct atgtttaaag caatcagaag
   125101 aattacagcg ctcgttgctt taattgctgt tgctataaaa ggcgctctct cattcttatc
   125161 ttgggttgaa aagcaggaag atgttgaagc agtttggtcc gaagacgagg atttagaaga
   125221 agctttctag tgagcgatac ttatattcct ggaacttgta atctaggcaa agccgaagtt
   125281 cgtagccgcc aaatagttgc acttgttggt cttgtcgctt cactagttct ggcaactggc
   125341 ttaattgtca gctctgctcc tcaagcctca ggattaactc tctttgcccc acttatggtc
   125401 tttgcggtcg gtttcattca atctaggaga aaattctgtc ttgcctatgg ccttgctgga
   125461 actttcaact tgggaaaact cggtcagatt tcaaaggtcg caaatccaga agataaagca
   125521 gcagatcgca aaaccgcact ttcaatcctt gctcaagcaa cagcgctagc gctagggcta
   125581 actggagcaa ttactctcct gctgctgtaa aaaaattcag gcttaattct cagttaatta
   125641 tcgcttatta ctcttgcctg cgctgcagag ataggagtac gttggcatag ttacaagctt
   125701 gataatgctc taggaatcgt taggagagcg atgaaaatca taattcataa ccatggagat
   125761 tcattagcaa tagattttga aaagattgca gttgagcgct tagagaggtt aactagattt
   125821 agtattccaa ttgagagaat tgatgtcgat gtaaaacatg aggcaaaccc aaggtttgga
   125881 aagagctctc atcgagtaat tctcacctct cgtggaagtg ggccacttct tcgagcagag
   125941 ggttcaggct ttaatgattt agcagcattt gatgaggcgg ccgaagctgt tgaacttcaa
   126001 ctacgaaagc gccacgagcg aagcaaagat attgatcgaa ctagccttcg aaagctgcgt
   126061 ggcgcaaaga attagcaaga gtgctaatgg caaggaagcg ctgccttaga cttatcttct
   126121 aacttttcct cattttagga aggcagagcg cgtgattgaa gatctagata aagcgctatc
   126181 acgcgcgcaa gatgtattag ctagcccaga atcaattagg cggatctgca tttcaggaaa
   126241 ggcaaagggc aaacaaccag agcaagtgcg cattgatatt cgcccagttg ctcttaaggc
   126301 agggctgcat tggcaggtag taagccatga tggaaagcgc gacacaacaa agaatcttgc
   126361 tctaggtgag ctctctttag ccaggttgtt taatttaggt tattcaaaca tattgattga
   126421 gagtacaagc caggagataa accttcgatt aacaaagtca ggtgatgctc aattttcaac
   126481 aaagcgagtt gagctagatg cagctgagct aactcacgat agaacaaaag agcggctatt
   126541 gagcgcagat gatgagatat ttatcgagct aggcatctca gaccagaatg gaaaactaaa
   126601 gcccagtaga agcgataagt ttattcaggt tcaagaattt ctaaagatct tgagtcatag
   126661 ccttaatgaa aagcgagata agaaccaaga actaaaagtc attgatctgg gctgcggcca
   126721 tgcttatcta acccttgcag cccataagta tctaagtaag cagggatata aggtgaaaac
   126781 cttaggtatt gatgagaggc aagacagtag gaagcgaaat atagctctgg ttgagaaatt
   126841 gaaaatgagt aaagaaatta catttcaggc aacgaaaata gcaaacctag agttagctaa
   126901 ctttgatatt gcaattgctc ttcatgcctg tgatacggca agtgatgatg cgatttcttg
   126961 ggcagttaaa agtggggtag agatgatttt ggttgcccct tgctgccatc atgatattca
   127021 aagacagatg aaagaggcgc cagctccatg gaatattgct actagacatg gaattattaa
   127081 tgagagagtg ggagatattc tcactgattc aataagagca ggcgtgctaa agattttggg
   127141 atatcgcacc gatatcatcg aatttgttgc tggggagcac acaccaagaa atctgatgat
   127201 ccgcgctttc aaaactggag ctccggcgca gagggctgat attgctgagt tagagcagat
   127261 tattacgcag tggaagattg agcctgcctt aatggttcgc cttaaagaag agctctcagt
   127321 taggcttggc taatgtcctc aaagcaatct agtggcaaag ttttagcctc acttcccgtt
   127381 gggcaaaaag ttggaattgc tttttctgga ggccttgata cctcagttgc agttgcatgg
   127441 atgaggcaga aaggagcgct tccttgtgct tacaccgcag atcttggtca atacgatgag
   127501 agcaacattg aaagtatcgc ttcaagagct aaagagtatg gagcagagat tgctcgcctt
   127561 attgattgca agaactcttt agttgaagag ggccttgctg ctttagcatc gggcgctttt
   127621 catattagaa gtgctggaaa gatttacttc aacaccactc ctcttggaag agctgttacc
   127681 ggaacacttc tagttcgagc gatgcttgaa gataacgttt tgatttgggg agatggctca
   127741 acctataaag gaaatgacat agagcgtttc tatcgctatg gcctgctagc aaatcctgag
   127801 ctaaagattt ataagccatg gcttgatagc gattttgtcg cagagcttgg tggtcgaaaa
   127861 gagatgtcag attggcttaa gagccacaat ctgccttatc gagatagcgc tgagaaggct
   127921 tactcaacag atgcaaatat cttgggagca actcatgagg ctaagaaact tgaggagcta
   127981 agtagttcta ttgagattgt tgagccaatt atgggagtta agttctggga tcaagcagtg
   128041 gcaatagccc aggaagatgt gaaaataacc tttaagtcag gaagaccggt tgcaataaat
   128101 aataaagatt tcagtgatgt agttgagttg atgaggcaag ctaatttaat cggtggccga
   128161 catggtcttg gaatgtcaga tcagattgaa aatcggatca ttgaggcaaa gagtcgaggt
   128221 atttatgaag cacctggaat ggcgctgcta tttattgcct atgagcgcct tcttagcgca
   128281 gttcacaatg aagagactct ggctaattat tatcaatcag gaagaaagct tggacgcctt
   128341 ctttatgagg gaagatggct cgatccacaa tctctaatgc ttagagaatc actaactcgc
   128401 tgggtggcat cggctgtaag cggtgaagtg gtcttaaggc taaggcgagg cgatgattat
   128461 tcaattattg atacaaaagg tgagaacttt agttatcacc cagagaaact ctcaatggag
   128521 agaacgcagg cagcagcctt tggaccagag gatcgaattg ggcaattaac catgcgtaat
   128581 cttgatattg cagatactag acagaagctt gaaatgtatc gagaccaggg ccagattggt
   128641 aagggaaact ttgatttagt agagattgaa aatcaggaga aaaaagagac gaagggtaaa
   128701 taaatgacaa ccagtgcgat aacaaagatt ctcgataact ttctagagga gggaatcaag
   128761 ctatcgccaa ttggggcaac catgcttggg gttcctggtt ttgatgatca actagatgac
   128821 ctatccatgg caggcaatga acagcgtgcg gagctaactc gaaagactct tgctgcaata
   128881 aagaatgaaa ctccgattaa tgaatttgat cgaatcgcca aagatgtggc agtagagcgc
   128941 ctaacctccg agttaaacct caatgacacc tttgaggcgc gaattctctt taatctcatc
   129001 tcatctccgg taacaagcat tcgacaagtc tttgagatga tgaagccaga tcaagcagag
   129061 acggtctcaa atgtcacaaa gcgccttaat ggaattgaga cagcttttgc gggatggaaa
   129121 tcatcattgg aatatgtggc agctaaagga aaattcaatt cccgcagaca agttcttgca
   129181 accgctggtc aattagagac tttttcaaag ggtgcattta ctgccgttgc aaagaaattc
   129241 aatcctgcag gtgataatca agctttattg gctgcagcaa agagcgctga ggcggcttgc
   129301 gcatcacttt catcctggat gcgagattcg tatgcgccta aatgtccagc agttgatggc
   129361 gtaggcgagg agagatacaa gatgtgggct cgccatttca cgggagcaaa tcttgacctg
   129421 cgtgatacct atgagtgggg aatcaaacaa ctagagatga tcaatgagcg gatgtggagc
   129481 gcagcaaaga agctatatcc agatgcaaaa actcttaggg aagttgcaga tcgccttgaa
   129541 gcagatccga gatacaccgt tgtcggcgaa gaagaattgc ttaaaaaact taaggagttc
   129601 atagctgctg ctgttgagag acttgatgga aaagaatttg atatcgatcc aagaattcga
   129661 aactgcgaag cacgccttgc tccgccagga ggcgcctctg ctgcctatta catgggacca
   129721 agtgaggatt tatcaagacc tggaacaact tggtatccaa ccatgggaag aaagactttt
   129781 ggatggtggc atatcgtttc aacttggtat cacgaagctg ttccaggaca tcacttgcaa
   129841 gttgcaacaa caaagataaa caccgatcgt ctcaatcgtt tccaacgcaa cagagtttgg
   129901 gtctcaggat atggcgaagg ttgggcgctt tatgcagagc gcttgatgga tgaacttggc
   129961 gcatttgaag atccaggtta tgagatggga tatctctctg cgcaagggct aagagcggca
   130021 cgcatcgttg ttgatattgg aatgcattgt ggttataaag actttaatgg tgagatttgg
   130081 aacgctgagt ctgcatttaa acttctccat gagagagctt tgcttgatga aatctctgca
   130141 cgaagtgaag tggatcgcta tctcggttgg ccaggacagg caatttctta caaagttgga
   130201 gagcgcttca tgatggaggc aagggaagat gcaaagaaga ggcttggagc tacctttaat
   130261 ctaaagaagt tccactctta tgtattaaat cttggaccaa tgggtcttga tccatttaag
   130321 gctgaaatgg ccgcttggga cggaaagtaa ttctcggcta gaatcaccct gttgtttccg
   130381 gggtcggtgt aattccgaac cggcagttaa agtctgcgac ccgcttgcat ccagctagcg
   130441 gtggacttgg tgaaattcca agaccgacgg ttaaagtccg gatgagagga aacaaattgc
   130501 gtgcatcctt gcgcgcactc ttttgcgcac cccggaaatc tatttggggg gtggcgataa
   130561 tgataaatgc agaatctgca atgcgccgtg ccaacgaaat ttcaaaacta ggtcttggta
   130621 agagttctcc taatccaatt gttggagcag tgattgttgg caatgatggc cagattgctg
   130681 gtgaaggttt tcatcacagc caggatggtg gaaaacatgc tgaagttatt gcaattagta
   130741 gagctggaca gagggcaaat ggtgcaacgc tttttgtaac gctagagcct tgtaatcata
   130801 tgggaaaaac gccgccctgc actgaggcaa taattgctgc aggcataaag aaagtagttt
   130861 ttgcagttag cgatccaaac ccaatagccc atgggggagc gcaagcacta gctgatgcag
   130921 gcataattgt tgaacaaggt cttttaaagg atgaagttgc cttcaccaat agggcatggc
   130981 taaaaaagat agagagctcg catccctaca taactataaa gattgcagca agccttgatg
   131041 ggaaagttgc tgcgctagat ggaagctcaa agtggattac aagtgagatt tcaagatctg
   131101 atgtggcaat tcttcgcagt caatgtgatg caattgttac tggcacagga acagttcttg
   131161 ccgatgatcc ctctctaacg gttagaaata tcaagcgtga gaactttgag ttcaagcccg
   131221 tgcgggtaat acttggaaaa cgagagattc cgcttgattc aaaggtttta gataagagcg
   131281 ctgaaaccat tcaaataaag ggaaatgatc taggcgagtt aatggaattg gcaaaagagc
   131341 gcgcctggaa tcagatactt attgaagccg gcccaacttt aactagcgca tttctcaagg
   131401 ctggactatt tgatgaagta tttctctatc aagcgccaac tcttcttgga tcaggtcttg
   131461 attttgctag ccagcttgag attaaaaccc ttaatcaaag attagatctt gagcttcttt
   131521 cagcggattt acttggagat aaggaaaaga atctaaggct tcatttattg gcggtgagca
   131581 aataatgttt acaggtcttg ttgctgataa agctaaggta atctcaattg ctagaggcgc
   131641 agaatctgcc gtaattcaaa ttgagagcgt gcttacccct gaaataaagg taggggattc
   131701 agtgagcatc aatggaactt gcctaactgc aattgaattg agctcaaata cttttaaagc
   131761 cgatgtgatg attcaaacgc tcaaggtgac aagtcttgga aaacttagcg aaggtgactt
   131821 tgttaatctt gagctagcca ctagggcgga ttcaagactt ggtggacata ttgttcaagg
   131881 acatgtggat ggaattggca gtgttataga gaactcacca ggtgagaagt ggaataagtt
   131941 tgtagttaga gttcctaaaa acttaaccaa atacatagtg aatcagggct ctattgcaat
   132001 tgatggagtt tcacttactg tgggcgagat atcggatgac ttactaacgc tctggcttat
   132061 tccagagact cttgagagaa ctaatctttt ccagaaatct agtggggatg ttgtaaatat
   132121 tgaagttgat gttattgcta aatatgttga gcgccttatg aatagaggtc agtcgtgact
   132181 ctttcattag ataaaacact tgaggccttt agagatggcg gctttgtaat tgttactgac
   132241 gatgcagatc gagaaaatga aggtgaccta ttccttcttg ctagcgccgc cacaacagag
   132301 aagattggct ttatgatccg ctacacctct ggtgttatct gtgtagcaat gactgaggag
   132361 cgctcaaggc agttgcatct gccacttatg gttaaacaga atcaagatac aaagcgaact
   132421 gcatttaccg taacagttga tgctaagcat gagataacca ctggaatcag cgcaaaagag
   132481 cgcgctaaca caatacgagc tctagcagat ttaagctcca cagcagagga cttcataaga
   132541 cctggccata tcttcccgct cattgcccat gaaggcggtc ttgctgcaag acgcgggcac
   132601 accgaagcca tcgttgagat gtgcaagttg gtcggtgaaa accaagttgg agttattagt
   132661 gagttagtca atgaagatgg ttcgatgatg cgtggtgaaa atctaactag attcgccagt
   132721 gaacaaggta ttcctatctt aagtattgcc cagcttatga gcgccctaga aggttcaaat
   132781 aaattagata gccagaagga atttcatctt aattgggcag agcttccacg aagtagttct
   132841 ggccagatta agcagtggca gattgcaact ttcctgggtc gaggtggagt tgatcatgcg
   132901 ttattgaagt ttggaattct aaactcagag aaaccacttc tagtgcgaat tcactcagag
   132961 tgtttaactg gcgatgtact gggctcactt cgctgtgatt gtggatcaca actagagcgg
   133021 gctatgaatg agattgaaag cgctggctct gggttaatca tctatcttag agatcaagag
   133081 ggacgaggca ttggactatc cgaaaaaatc aaggcttatc aattgcaaga tcaaggcctt
   133141 gataccgttg atgccaatct agctcttggt cacaatattg atgaaagaga ctttggtgat
   133201 gcagctgaaa tccttaactc tttgaaaatc aattcggtag tcttgttgag caataaccct
   133261 gaaaagctag aaactcttac ttcaagtggc atcaaggcga gccttcaaat tctttccgga
   133321 gatatcaata cctttaatga gaaatatgtg gcaaccaaaa aagagagatt ggggcataaa
   133381 taatgtcagg tgtagttcca aaagtatcac ttggtgactt aggtcaatat agggttgcaa
   133441 tcatctctgc tagttggcat gagaagatct gcgctgattt aatcgctggc gctagaaaag
   133501 cgttagattt ggcaaaaatt aagccagaac cagtcatata tgtccctggc tcttttgaat
   133561 tacctcttgc tgctcaatta gctcttgata gcggctttga tgctgcagtg gttctaggag
   133621 tggttctaag aggtgaaact ccacactttg attatgtatg tcagggagtg actcaaggga
   133681 ttatgcaagt atctctaaca agatccaagc cgattggctt tggagtttta accgtcgaca
   133741 ccgttgagca agcaattgca agaagtggaa tcactggaag caaggaagat aaaggctttg
   133801 attcagcagt tgcagcactt gacttactta gagtgaagag cgaattacct gttctgagga
   133861 aatcctagat ttatcccgcc atggcttgga tcaagccagc ggctcgtaac taccttgcct
   133921 ctagtaaaga aatgaacgcc ttccattcca tgcgcgtgag tgtctccaaa tagtgagttc
   133981 ttccagccac caaaggagta ataagcagtg ggcacaggaa ttggcacatt aatcccaatc
   134041 attccaacct gaacttcatt ttggaatctt ctcgcagctc caccatcatt ggtaaaaatc
   134101 gcagttccat ttccatattg atgcttgttt attaactcaa ggccctggtc ataacttgat
   134161 actcgaataa ctgagagaac tggtccaaag atttcatcct tataaatact catctcaggg
   134221 gtaacttgat caaagagagt tggtccaagc cagaaaccct ctgctgctcc actaactttt
   134281 ggattacgac catcgacaac taactttgct ccagcactta ctcctgcatc gatataggag
   134341 gcaactttgt cgcgatgaac agcggtaaca agagggccca tatctgatcc tgcgctgcca
   134401 tctcctgttt ttaggtttgt catgcgttct gaaattttct taattaactc atcagcaaca
   134461 ggttcaacaa caactagagc cgagattgcc atgcaacgct caccagcgga gccaaagcca
   134521 gcatttactg ctgcatctgc tgctagatca agatcagcat caggaaggac caacatatga
   134581 tttttcgccc cgccgagtgc ttgaactcgc ttgccatttt tagtgccatt ttcatagata
   134641 tatcgagcaa taggagttga tcccacaaaa gaaattgaag caacgtctgg atgagaaaga
   134701 aggctatcta ctgcaacttt atcgccatgg ataacattaa atactccatc aggaagacct
   134761 gcctctttcc agaattctgc aacgagatta atagcagatg ggtctttctc gctgggcttg
   134821 ataatgactg catttcctgc agcgattgcg attgggaaaa accacatcgg aaccatcgct
   134881 ggaaaattaa agggagagat gatggcgcaa actccaagtg gctgacgaat tgaatagaca
   134941 tcaatgtttg tagaagctcc ttcagagtat gcacctttaa gtagatgggg gataccgcag
   135001 gcaaactcca ccacttcaag tcctctagtt acctcaccta atgcatctga taagactttt
   135061 ccatgctcag cggtgattaa tttcgctacc tcttcctttc gagcatttaa gatttctcta
   135121 aaagcaaaga gcacttgaac tctcttagca agagatgctt gagcccattg ctcttgagct
   135181 ttctttgcaa ctttaacgcc atgatcaatt agctccggcg ttgcaaaatc aacctttgca
   135241 gtaactcttc ctgttgctgg gttatagaca tcaccacttc ttgtagcgct ctctgtccaa
   135301 taggcgccat ttatgtgatg ggtaattctc tttacttcac tcatctttat tttgccccgg
   135361 tgtagtactt atcaatctga gtaaatgctt tatcaagtat tgcaagtcct tcgttaaatt
   135421 ctgcctcagt gatattgcac ggtggcacta catgcatacg attaaagttg gtaaaaggca
   135481 ttaaaccaag cttcttgcat tcacccacta gctcattcat cgcaggagat gatccgccat
   135541 aaggggcaag tggcgctctg ctggcgcgat ctgtcactaa atcaagggcc cagaaggttc
   135601 cttggcctct aacttctcca atgactggat gcttgcttgc taaatcacga agacctggac
   135661 caattacctt ctcgccaatt gtggctgcag cttcaacgat cttctcatct ttcatggcat
   135721 caatagttgc aactgctgcg gcgcaagcaa gcggatggcc ggagtaggta aggccgccag
   135781 gaaatactcg atctttaaag gtctctgcaa tctcttttga gattacaacc ccaccaagtg
   135841 ggacatatcc agaattaact cccttagcaa agacgattaa atctggatga gcacttgaat
   135901 gttggtaggc aaaccacttt ccagttcttc caaatcctga catcacttca tctgctatcc
   135961 ataaaattcc atacttatcg cagaggcgcc ttactccctc taaatatccc tttggtggaa
   136021 ttaagactcc tgcggtgcca acaacagatt caatcaagat agctgcaatt gtctttggtc
   136081 cttcaaattc aataacgctc tccagatgct caagggctct ctggcattct tcctcttcgc
   136141 tctttgccca gaaagcactt cgataaagat atggaccaaa gaagtggaca tggccatagc
   136201 caaattcatt tggccatctt cttggatcac ccgttgctga aatagcagag gtggtgttgc
   136261 catgatagga gcgataaaag gagagaattt tatggcgtga ggtatggatg cgagccattc
   136321 tgatcgcatt ttctatgcca tctgctccgc cattggtgaa gaaaaccttt tcatgatttg
   136381 gacttgcaag gctgacaatt ctggaggcag cttctccgcg cgcctcattt gctgcttgtg
   136441 gagcaatagt tgttagtaga tctgcttgcc tcttaatagc atcaattact tttggatgtt
   136501 gaaatccaat attggtaaag actagttggc aggagaaatc taagtacttt tttccatcaa
   136561 aatcccaaaa gtatgagccg ctaccacccg caattggaag tggtgttatt tgtccctgag
   136621 cagaccaaga gtgaaacaca tattttctat caaggtcata gacctctttg cctctagcgg
   136681 gattggggat gaactcggcc attgcgctat ctaatcagcc cagtgcgatt tgctcaagca
   136741 gcggtgggga cccttaatgg ttatttgagg taacaacacc ttggtggata cacactagat
   136801 tcttttcatc cttgccacat ttttcgcaga gcaggaatag attatggcaa taaacatttg
   136861 cacagttatg aaattgcttg gttggctcgc tgcacttatc acacttgcca attaatttcg
   136921 tggactcaga aaaatcaatc accatgcgct tatcaaaggt gtatagcgag ccttgccaga
   136981 gtccctgatc cgcatatttc tcaccatagc gcactatccc gccagcaatt tgatagacct
   137041 tatcaaatcc acgctccttc ataacttttg ttaatatctc acagcggatt ccgccagtgc
   137101 agtaggtaac tacagtcttt tctttcaagt aatcgtattt gccgctatct agctctgcta
   137161 caaagtcttt tgtagtctta atgtcaggaa tcactgcatc tttgaagcgc cccactttag
   137221 cttcataggc atttcgtcca tcaaagaaaa ctaggtcctc acccaattgc ttagctagct
   137281 catttacttc ttcaggggag aggtgttctc ctcctccaat aatgcctttt tcatttactt
   137341 ctagattttc tggtatacca aaagtgacca actcatctcg caccttgacg ctaagttttg
   137401 gaaaatcact tccagtgcct tgcgaccatt taaaatcaat cttcttaaaa cctggatatt
   137461 gcttagtagc tctcacatac ttcttcaagt catccatgtc accgcctaaa gtgccgttaa
   137521 tgccatggcg actaataatg atacgaccct tgagatttag agattgagca agggtgtttt
   137581 gccaaagtct gagagcttca ggatctgcaa ctggggcaaa gccgtaatag agaatgatct
   137641 tgggcaacac agctctaagt ttaatatcgg ctcggcagat tgaaaactac gcaatttagg
   137701 gtcagaatca aggtatggca agtaaaagcg caaagagtaa gcaagtagca aaacgaaaga
   137761 ctgtttctcc aaggccaagt tccccagcac agaggcaaag tgaaccggcg attgctaagc
   137821 gtgaagatcg tattggttgg tattcactct atatctatgc ggtctgccta gttaccttac
   137881 taacctgctt attttcgttg gtaagtgtag ttcggggaat agtcgatgcg ctatggccag
   137941 atcctggtta ttttgatccc tactcagttc ctaaagattc agctttaagc cctgatcaga
   138001 ttaaagagaa tttgactgag aataatcaac gtcaagcact taagggaatc gttaattcac
   138061 taaccaccct aattattgct ggacctattt atcttatgca ctggcgcctt gcaaagcgct
   138121 ctagggctat ttaagtaaga gattagctaa gatctactct cgactcttct ttcctgcttt
   138181 tccagtagca aaagcaaaga gagcaagtcc tgcaaccaga gcgcctgcac caacgccgta
   138241 tgcaataacg tcatcgcctc ggtttgaatc aacgaataca cctacataga ccacggcaat
   138301 gattgccaca actacgccaa ttaatttaga ttttagatcg tctagatctt caactcgaag
   138361 ccatagtggc acatcaatat cagtatcgat aaatagttca tagagaccat agccaatcac
   138421 cagaagtact gtgccaagca agatggcatc aattgcagtt aggacttcaa caattgtttc
   138481 cttgagacca tattgactct caagtgaatc aataactgct aggaccatag caaaggatcc
   138541 ttgagccata agaagtaatg cgccaagaat tgaggcaagg gcaggaataa ctactgcata
   138601 tcttgtaagt cctagtaggc gcttcataac tgtctttagg gtatctgaaa caaactcaaa
   138661 attacctcga gcgaggtgat aattcatcac tttttcaacg cgaccaatac agaagtgggt
   138721 tgtgtaggga tcgaacctac gacccggtga ttaagagtca cctgctctac caactgagct
   138781 aacaacccgt tgaccatttg tcgtgatcaa gcggagcaca ctataaccag taccgcctta
   138841 gataccaatt tctaagccaa atagatgaga atgaagccat gaagtccaat tggcaaaaag
   138901 ccatttttag ccttttgaag tcaaaaaaag agctgaaaca cagagcagct tttacttcac
   138961 aagaaactct gatagttcta atcgcaagct ttgcacttac ttctttctct cttgtatcac
   139021 cgagctcgaa atatcagatt gaaatactag aacttgcttc agctaatgga gcagagtttg
   139081 atccaattaa ggcacttgaa actctggcag tgaaaggacg ggcgccaaag acgggatatt
   139141 caagatctgc ctttggtcct gcctgggcag atgtggatag aaatggctgt gatactagaa
   139201 atgacattct taaaagagat ttaactcaag tcaccttcag agcaaaaacc aaagattgtg
   139261 ttgtggaaag tggagtgctt ttagatccct tctctggcga aagcattaat tttcaaaggg
   139321 gagaaaaaac aagcacactt gttcagatcg atcatgttgt cgcgctatct aatgcctggc
   139381 agacgggaat ctttaaggca gatcttgcta ctaggaagaa ctttgctaat gacccgctaa
   139441 atcttctagc agttaaaggt tctctaaata gccagaaagg tgatggtgat gctgcaactt
   139501 ggcttccacc gaataagcaa tttcgctgtg attatgtttc aagacaagtt gaggtaaaag
   139561 ttaagtacgg cctttgggta acaagagcag agaaagatgc aaccttaaga atcttatcta
   139621 acccgccctg cctaaagcag aattaatctc ttaactcttc tgcagatgac ttggggttat
   139681 ctctggaaac caggagagag cttcatctct gaaatttcct tcagctctta attggcaaaa
   139741 tattccagta gttccaagag tgactctatg aattaagaca tattccggag gaagatttaa
   139801 ttgaaaacca atctttgcag ttggatttct tggatccccc actcgcacgc tctgagttct
   139861 aagccagtcg cggctatagg cgaaataatc agttcttaaa ggctcaacta gcggaagtag
   139921 ataatcaaga actaactcag ggctgacctc aacatcggcg agaatgaagc catcttcttt
   139981 aaagccgtta tataaagcaa tcgcatcacc ttcaagcgca tttctaagta agcgtttaaa
   140041 aggttctgga aatccattag gcagacgatt acatgcgcca aaatctagaa ctccaaggcg
   140101 accatctttt aagactctaa agtttccagg atgaggatct gcatgtaata aacctgctct
   140161 cataggagca gtgaagtgga aacgagctag gcgaatgccg gcgctatttc gctcttcttg
   140221 tgtgccatcg gtaattacct ttgatagcgg agttccctcg agccactggc ttacaagcac
   140281 tcgatcagtt gccataatca cttcaggaat tgcaatatcg ctatcacctt tataggcctc
   140341 aaagcaggcg ctctgcgcac ttgcttcata gcgataatca acttcttcaa taattcttgc
   140401 cttgagctct tctagtaatg gtttcatctc aacgccagga agaagtaatt ggaaaatctt
   140461 tgcaaagcgt tgaatctgat tgagatcaga aattagcgcc tctttagctc cgggatactg
   140521 aatctttact gcaactgctc tgccatcttt ccaaatccct ttatgaactt gtccaattga
   140581 tgctgaggct gctggggtgt cattaaattc tgcaaaatta tctcgccaat gctcgcctag
   140641 ctcttttgct aatactttat gaaccactct tgctggaagt gggggagcag cctcctgcag
   140701 ctttactagc gtctcgcgat atggctttgc aatatcttca ggcagtgctg cctcaaagac
   140761 agatagggct tggccaaact tcattgctcc gccctttaac tctcccaata ctttaaagat
   140821 ttgctctgcg gtcttttctt gcaaatctgc aaaggcaaaa tctggtgatt gaccaattaa
   140881 ttgacggcca aagccaatgg ctccgcggcc agcaagagat aaggggatgc tggccatctt
   140941 tgcgcttcgt ttgctggttc cctgcgggag ctcttccgac accggcatat tcttatctag
   141001 cctgggatat agcgcgaatt gaaactagcg ccagttgcag ccgcagccag gattaattga
   141061 ccacttctcc aactttgggt tatgaaaatt tgagttggag tagatcaaag attttcctag
   141121 aaagtggctt atgccacttg aggcaaggct aattacctca agacaagtaa tcgcagcagt
   141181 aactagagca agagctgatc ccacctcttt tctctctact gaacttaact tctcttcact
   141241 agatatttgc agacagcgat aacaagggct ctttcctggg attacatatg gcccaattcg
   141301 aacttcggct gatgaatggg gctcaatgat taagtggtta ctaccttcag agatccaacg
   141361 ttgtaataca tctggtgttg gccgaccaac gctaattatt agccttggcc gataaattac
   141421 tggcgtagcc tcagcaaaga gcgcggaaga acttcgagct tttgccaaga cttcttttct
   141481 tgatgctccg atatcagctc ttgagatata tccgccagag agatcacttt ctaaaatctt
   141541 taaagcgcta gatctctgct gcagacgatt aattactaag accttggtaa atcctgaggc
   141601 gctaagtaat ccaactagcg agttaacaat ttttccatag ccaaaaatca ggattgagaa
   141661 atcgcggcgc cttacaactg cgcttcttcc tccatcgata tctccttttt caagagatgc
   141721 cgcctcagat tcaatctcca ttcgtttgat aaaggattga gcagcgaggt cttcattaaa
   141781 gtcttcaagg tctgtaacag gagtaattgc tttgactctt gggtcatatc gctttaggta
   141841 tgagatagcg gttcgctcta gatcgattaa gtcatgtgct tgcagctcat caacgagtgc
   141901 ttggagttga gagatagtaa gagcgcactg cttgctaatc tgctcaaggc ttaattggcc
   141961 attaaaactc tccagcaata accgagttga gattccaaac cagaggctcg gtaggcatat
   142021 ggatttatag ggcgagccga taaatacctg agcgccatta gggctgagcc agatgctaag
   142081 cgcactttta atcgccggcc gtgattccat aagggggcga tcatctagag ccattaggga
   142141 atgtacctgc atcaaaggaa ctgagctggg gaggggcgct tgaatctaat taacttggta
   142201 tgcctgggac ttactaagag tgtttagatg cgaaaataaa taaagcgccc acagctaatg
   142261 ctgcgagcgc tctattttta aaagctggtt taagccttgc ctaaaatgcg attgacctta
   142321 gttccgcagg ttgggcagat gccctgtgcc atgcgacggc ctgattcgga gacctttacg
   142381 gttccttcga attcacgctt agccttgcac tttacgcagt aggcctcacc tttatatgtc
   142441 tctgccatag ctatcctccg atcctcgcag cgctgggata gcctgcgaat aggctcacca
   142501 tacccccgca taccctcaac tcaggcgctc cccacgctca atggggatat tttttcttcc
   142561 tagttccccg ctcgaacccc tgcttcatag ccctcaaggt aggcgctagc ctcttttccg
   142621 agctcaaagc gagctagaag ggcctcaaag tctgggccat gatcggcaat catcaagtga
   142681 atgagttcat ggactagaag gtagtcaaga acatagccag gagcgccatt gaggcggtct
   142741 gagatacgga tgctccgctc aactgtggtg caggagcccc aacgctcgct catggagcgc
   142801 caggtaacag agacgggtct aactgggcac tctggaagat atttagcgag aagttctagt
   142861 gcgcgcttat acaaatcttc ttcgtctatc ttctctctag cctcaagtga gagtactttg
   142921 ctcaccatct caggaatcac ttggcgaacc tcgctcttag ataagcgagc tggaactgag
   142981 ataacgattg ctcctgcttg ccggtaggcg ctaattgatt tctttcgccg cgtgcttcta
   143041 attaccttca cctcggctcc attagagctt ggagttggtg gaattttgat tactgaagct
   143101 gctctctggc cgtcatctcg catctggggc aggttatcgt tgaagatttc atcttggatg
   143161 aagttttcca caacgcaacc tttctaaaga ggtattacca ctacaaactc tataggagta
   143221 gatgagtctt tagggttgaa aaactcgcca gttgaagttg attatttata tctatctgtc
   143281 gtactttgcg cctacgaaga cctcggaaaa ctctttcata tctgcaaggg gaaggcagat
   143341 aagaaatgtg cgaccggggt cttcgctttt attcctaaat caaacctgag agatcatctg
   143401 ggatttttct agacttagaa aagccttccg gatctaatag ctgagatgcg gtgggaagca
   143461 ggaatgcctc ttcccaaatt ttatctcttg cagagattga atcaattttc tcaatctttt
   143521 cccagaatga aatagcttct ctagttaatc ttggtgaaac ttcaagaccg agaagagttg
   143581 caaataattg ttgagttgga gaattggtag cgcgccttct tgcctgtgat tctcttaatt
   143641 taatcagcga tggaagacga tctgttgcag cctttgcaac aactgcatca atccatcctt
   143701 caatgagtgc aaggagtgtc tctaactttt ctagagcagc gctctgtgaa gcagatgtct
   143761 ctggagtaaa gagaccgcct gaaagagccg atgccattga ttctggatta cttggatcaa
   143821 ttgcgccaga gtccattgca tcttgagcgc tctgtgtaat tgcgtttata tcaatacgaa
   143881 ttccactgcc atatgccgaa attacttcac cgatatatcc gctaagccag gggctctgtt
   143941 tgaagaggcg ggcagcagca atctctctta aggcgagata aattcttatc tcagcttctt
   144001 ggatttcaag acctgagccc caagccctaa cattttgtgg aagtaattga gcctttatat
   144061 ctttacttag aggaagagct acatcatttg cgccagtaac tgtggttgaa agcgcgccaa
   144121 tactctggcc caattgcgtg ctaataagtg agctcataaa ggttgccatg atggcgttga
   144181 tacttgattt agagattgac attgggccaa aaccaggaat tgaaattccg ttatcttcgc
   144241 cctgcccatc tagcccttga tcaagcatcg acgagagagc agctgacatc gaatcaacaa
   144301 gtggcttagt gagctcttgc cagccattta aagtgctatc aatccaatct cttcttgata
   144361 gcgcaattga atctgttagg ggcagagctg gaaaggttgt ggcctcatta atccagaggt
   144421 ctgcaatatt tatggcttct tgaatctggg cgagatcttc aacagttact ggaacttcgc
   144481 catgggcact taaatacttt cttgagatat cacggatcat ctcctttgat agcgaggcag
   144541 gagatgcttg gccagatagg gatgccataa gagctggaag atttaaaccc attccggaga
   144601 attgcttaaa gatttctcca aagttttcaa agccaaaggg gttattacct tttgactcat
   144661 cctcatcacc gttatctggc gtaaagccaa aacctggcat cttaaaaaac tccctctaca
   144721 taagttttaa gtattacggc ttatctcaaa ggccagtaaa tctgatctct tgactaggag
   144781 aacaaatcta gcccagcttt attcccaagg cctgggggag aatatgcttc aaattactaa
   144841 ttagcctcac ctagcctagg cttgagccat gagcgaagta atggcgatca aatgggctgc
   144901 tgaatctgcc tctgctccgc tatctgctct tatctggtgg tttattccgc ttaccgcggc
   144961 cgttggagct gccggctatg tcatctgggt aacaaagttt cagaagaaat atgagaataa
   145021 gactgagaga tcggtgggaa gatttcagag ttttcagaaa tcttttaaat caagtgaaag
   145081 tgataaagag tagctctaat gcgaaatatc tccccacttc gatatcgctg gtcttttcca
   145141 gttaaattct ttttctatct gttaaccatt gcaactctac ttgcgccaat tccttttata
   145201 ttctttaaac ctggggtgcc agataaagta actggaaaac taattcaggt aaatgatttt
   145261 aaaacttatc cagtaaatgg tgagttatat atcacctcaa ttttggtcac taatccagac
   145321 tctccggtct ttggcgcaga gaccatagtt aattgggcga ttggtgccaa tgtggttctg
   145381 ccaagagatg cggtctatcc acctgtaaag ccagcacaag ttattcagcg agacagtaga
   145441 agcgagatgg aatcatcaaa gatcacagca actgctgctg cgcttcgcta tctaggctat
   145501 gactttattg agctctattt cataagtgcc attagagatt actccaatgc caaagagaaa
   145561 tttaaagtcg gggatttcat taaggaaatt gatggcaaag taattggaga gatagaggag
   145621 attcgctcct cttatgccga aaaagatatt ggtgacccgc tgctaatagc agtggatcga
   145681 gagaattcaa agggcgagct tgagcgaatc accgatgaaa tcattctggt tgaaaatcaa
   145741 gaggtagtta atgaagatgg ttccaagcgt cctgccattg gaatactggt tggcaccacc
   145801 gcacgttttc caattgatat cgactttaat attcgaggag tcggcgggcc aagtgcaggt
   145861 ttaatttttg cagttggaat tattgaaaaa ctcaccgatg aagatctctt gagagggcga
   145921 aaggttgcag gcactggaac tattacccct tcaggccaag ttggagctat tggcggcata
   145981 gaagagaaga tgattggggc ctctcgaatt ggtgcgactg tctttattgc acctagagag
   146041 aattgcccag atattaaaaa tatcccagca ggccttaagg ttattccagt ttcaacatta
   146101 gctgaggcaa tcgccgctct tcgcgctcca gatagctttg tgcctcgttc ctgctcgaat
   146161 agttaagtta actatttttg gctaatctgc acccatgacc tttggagata acccagataa
   146221 tcctttccgc aacttccgct ttccttcaag aaacttaaat ggtggccaga ggccaaaaat
   146281 tggagccctg ccgctaacgg taattattct gaccgtactt gcagtgattc tggtttcctt
   146341 aagcggtttt tatgcagact ttctctggtt tagatctgtt gattacagcc aagtttggac
   146401 caccttactt acaactcgaa tcaccttatt tttcatcttt ggactattaa cttcattaat
   146461 tatcactgca aatatttata ttgcctataa gcgacgtcca atctatgtgc cagtaactgt
   146521 tgaggcagat aaccttgaga gataccgagc ccagcttgag ccacttagaa aagttggtct
   146581 aatcggtgtt gcagtagcgc tattttattt tgcaggaatg gcaggaacaa ggctctggga
   146641 gagctggtta ctttttagaa acgctacaga gtttggcgta gttgatccac agtttggtcg
   146701 agatatttca ttttttgcat ttgagcttcc cttctatcaa tcaatagttg gttgggctat
   146761 ttcaactctt atcttggcaa ccattgcatc tcttgcggtt cactatgttt atggcggtat
   146821 tagacctcag gtcagacaag atcgcaccac agttgctgcc agagttcagc tctcagtcct
   146881 acttggattt attgtggcaa ttaaggctgt ggcctactac ctcgatcgct ttgcacttgc
   146941 gacctctgat gaaggaataa tcaccggcct tacctatacc gatgtaaatg ccctccttcc
   147001 agcaaaatca atccttactg gaattgcagc actatgttcg ttgctatttt ttgccaacat
   147061 cattcgccgc tcctgggttc ttccagcagc aggggtagca cttcttggca tctcatcctt
   147121 tttaattgcc ggggtctatc cagggttaat ccagcaattt caagtaaagc caagtgagtc
   147181 ctcaagagag gctccatata ttcagagaaa cattgaagga actcgcgcag catatggcct
   147241 agataaggtg gaagttaagg attactcggc aattgttgat acatcagctg ggcaattggc
   147301 agatgatgca gcgacaattt caaatattcg tctaatggat ccaaatgtct tgtcagctac
   147361 tttccgccaa cttcaacaga tcaagcctta ttacaccttt aatgaatcac ttgatattga
   147421 tcgctacacc attgatgggg ttacccgaga tatggtcgtt gcagttcgag agataaatat
   147481 tgatggaaat ccaaatagaa attggatcaa tgatcatttg gtttataccc atggctttgg
   147541 ctttgtcggc tcatttggca atacccaaga tatcgacggt aagccagtct tttcagtggg
   147601 agatattcca ccaaaggggc tattaggtga tttccaacca agaatttatt ttggagagaa
   147661 caatccagag tattcaatta ttggtggcac cacagatggt gaggctgttg agtttgatta
   147721 tccagacgat gcttcagcta atggtcagaa gaattacacc tacaccggaa aaggtggagt
   147781 cccaatgggc tctatttttt caaggctgct atttgccatt aagtatcaag agcagagaat
   147841 cctgctttca aatctgataa atgctgatac caagattatc tttgaccgtg aaccacgtca
   147901 gagagtggca aaagttgctc catggctaaa gcttgatggc gatccatatc cagcaattgt
   147961 tgataataga attcaatgga ttatcgatgg atataccacc agctctggat atccatactc
   148021 aagaacagtt gatgtttcag gtgctacaac tgatgcgcta aacatcaata acaatccatt
   148081 aaccgcaatt cctaactcaa ccatcaacta cattcgaaac tctgttaaag caacagttga
   148141 tgcatatgac ggaacagtca ctctttatgc ctgggatgag aaagatccag ttctagcctc
   148201 ttggatgaaa gcatttcctg gaattgtgaa ggcaaaatct gagatgagca aagatttggt
   148261 ctctcatgtt cgctatccag aggatttgtt tagagtgcag cgagatgtgc tctccctcta
   148321 tcacgtaaag aatgcaaatg ccttctatgg tggacaagat ttctggcgcg taccaagaga
   148381 tccttcaact cttggcgcaa atgctggagc ccaacctcct tattactaca ccttgcaatt
   148441 gcctggtgag aagaaagcat cctttgcttt aacaactcca tttgtgccac gaggtggtcg
   148501 agaaaacctt tcagcatttg ctgttgtgaa ttcagatcct ggtgatgatt atggcaagtt
   148561 cacagttctg cagctgcaac gctcaacaaa cgtagcagga ccatcacagg ttgcttcaaa
   148621 ctttgaagcc aatccaacag ttgcgctatc gctatcactt cttcgccaag gtggctctga
   148681 tgttgtcctt ggaaaccttc taactcttcc agttggtggc ggtctgctct atgttcaacc
   148741 ggtttatgta agagcaactg caaatactgc tgcataccca ctcttgcaga aagtgctggt
   148801 ttcatttgga gagaaaattg gttttgatga cacacttaaa ggcgctctag atcaagtatt
   148861 tggtggaaat tctggaagca ctaatctgcc gcctgcaagt ggtggaagta ccggaagtaa
   148921 caccggatca aataatgatt tagcaagcgc acttgctagc gcgcaagcag ctcttgctga
   148981 tgcgcaggcc gcacttgcca agggcgactt tgctgcatac ggcaaagcgc aagatcgatt
   149041 aaaggcagcg attgccgcag cagttgcagc gcaaaacaga taatcgtttt ggctcaaaag
   149101 ggcttacgcc tctaggctaa gtcctccgac gcggggtgga gcagctcggt agctcgctgg
   149161 gctcataacc cagaggtcgt aggttcaaat cctgcccccg ctaccaaatt aaaaaagcgc
   149221 cttgaaaaat aaggcgcttt ttttcttgct caaattacag agggcttact tatctttctc
   149281 tttctttgga ccactcatcc ggccagcatg agcaatagca cttggatcgc gcttggattt
   149341 aattaagctg gcaactgttg caactatcag aattaagcaa attacgagca gcgaagtatt
   149401 tgtcgaaacc tttggaactt caacccagat ctcatgggcc cagagcagga ttaactttgc
   149461 cccgataaag atcaggataa atgctaagcc aagtgagaga tagatcagcc tctgtagtag
   149521 tcctttgatt aggaagtaga gagctctaag accaaggagt gcaaaggcat ttgcagcaaa
   149581 gaccaagaat ggttcctggg ttactccaaa ggttgccggg attgaatcaa gggcaaatag
   149641 aagatcagtt gagccaatgg caaccatcac tagaagaagt ggtgtggcat aacgcttacc
   149701 gttttcttta atgaacatct ttgttccatg atattgatcc gtgaaggcaa atttctttcg
   149761 ggcatagcgc actatcgcat tgtcatttag atcgggatcc tcatcccaat gctggaataa
   149821 cttgacccct gtccagagca atatcgcgcc gaaaatcaca aaagtaaatg aaaatgtagc
   149881 aagggcagct gctccgagtg ctataaatat cgcacgcata attaatgcaa gaacgattcc
   149941 taccatcaag accctctgat gatagataga tggaactgca aaatttgcca ggattatgac
   150001 gaatacaaag agattatcaa ctgataagga cttctcaact acgtaagcgg cgaaatactc
   150061 tttaccaaat tgatctccaa attcaatcca tacccatgcg ccaaagatga gggcaactgc
   150121 gatgtaaaac actgaccaaa aacttgcctc tttaaaggag acatcatggg ctttgcggga
   150181 ggaggccaat agatcaaaga ttaaaagtcc aactatgaat gcgacagttc caatccagat
   150241 ggaattagat aaattcacgc cgagagggta gcgctccgat taattcttag cgagtttaga
   150301 actactaaga gacttgatac tgccatcgcc ccaccggcat acattggatt tagtagtcct
   150361 aaagcggcaa ttggaatgcc gataacattg taaaaaaacg cccatcccag atttactctg
   150421 ataatcctca gagttttggc tgagagattt agcgcagaga ttgcagtagc taaagttgga
   150481 cgcattagtg tgatatcggc gctagatatt gcagtatcag ttcctgtgcc aagggccata
   150541 ctgagatcag cctcactaag tgctgcggca tcatttatgc catcaccaat catgagaact
   150601 tttcgaccag agctacgtag ctctttgacc tttgcaatct tttgatcggg agttgcttga
   150661 gcaatcacgt tgctaagagc aattcctacc tggtccgcaa tctgtgttgc agcctcttga
   150721 ctatcaccgc taattagcca gctatcaata tttctactct ttagagccgc aatagtttgc
   150781 ctagcatcaa ctcttaagct atctccaact tcgaagagcg ccatagcaac gccatctact
   150841 gcaactagcg ctactgaatt tccttttgaa agggcagatg agatggcatc ttctagttgg
   150901 gggtgaagcg aaacagttgc ccgcctgatt gaggcaggag ttccaataag aactggcatc
   150961 tgcgcttctc caaattgcat gcgagcagca attcccatgc ccgcgctctc tacataatcc
   151021 gtaaatttct ttctagcaat tccttgttct ctaaggctct tactgattgc aactgcaatc
   151081 ggatgactac tctcatgctc caggctgtga actgcaatta atagatctga tgttgaaatc
   151141 tttggcgcag ctgccccaag atcggcaatt gtgatgtttt gaaccttcat cacacctgtt
   151201 gtaagggtgc ccgtcttatc aaagatagcg gtatcaatct ttgttgcaat ctcaagtgat
   151261 gaactttttc gcaggataat tccattggta gcacctttgc cagatgccac aagaagcgct
   151321 actggggtag caagtcccag ggcgcaagga caggcgataa tcaatactgc tacagatgcg
   151381 gtgatggagt cagtcagcga atttcctaag ccataccaag tagcaaaagt tgcaatcgca
   151441 agtgcaataa caataggtac aaatacatta cttattcgat ctgcaagtct ttggataggg
   151501 gctttctcgc cttgggcggt taataccatc ttggtgatac gagcaagctc ggtatcactt
   151561 ccaactcttt ttgcctcaat aactattgga gaatttagat tgatagcgcc agcaataaca
   151621 cttgctccag cttgaacatc taccggcagg gattcacctg ttagtagcga attatcaata
   151681 gcgcttgatc cttgaatcac aattccatca gttggaatag cttggcctgc tttgactaag
   151741 cagtgatcac caattactaa attagcaatg ggcgccgtta tctcttggcc acctctaata
   151801 atgattactt gttttggagt gagggagagc aggtgggcaa gggatgatcc agcttttctc
   151861 ttggctcttg attcaagata tcttccaaga acaataaagg tgacaacaga tgcgccaact
   151921 tcagaataga tatcaccgtt tccagttgag ttggcataga tcgaccagat aagggcagtt
   151981 agggagccta atgaaattaa gttatccata cttggatggg tgatgttctt aatagccgct
   152041 cgatgaattg gccaaccaat taagagcaca actggagcgc taagagaaat tactagccag
   152101 ccagttgggg aatatagagg cgctgcgata ttgagtgaat ctaattgggt gagaataaat
   152161 ttatcaacct gcatatggag ttgatgccac atcgagagtg taattactgg aatggtaagc
   152221 aagatcgctg caattaatct aaagcccata gcccttgaat gagagaaact ctcataacca
   152281 tcgccgcgaa gctttgcgct gtagcctgct ttaacaactg cagctattaa atcttttgga
   152341 tttgcccctc gaggaatgag gatatgagcg ctatcggtag caagatttac tgaggcctta
   152401 gcccctggta cttcgtttag cgacttttcg actcttccaa cacatgaaga gcaggtcatg
   152461 ccagaaatac ttaaatcaat ttgttctaga tctactactt gagtatcact catgaattca
   152521 ccgcgctaat aaatgcttgg tgaagatgac cattggttga aactccgctt ttgccaaaag
   152581 gtccggcaac attttctaga tcgctaaatc ttcccccaga ttcggtaaca attatcgcaa
   152641 ccgccgccat atcccagagc gcaagacttg gctctgcggc aatatctacc gctccctctg
   152701 ccaccaacat atgtgaccag aaatcaccaa ttcctctagc tctccaggct ttatccataa
   152761 atgctaaata tgaattcttt cttggacccc aaccaactaa atctgaaaat gaaattgatg
   152821 catcagatat ttccttcaca tttgatgctt tgatttgaat tgcagcacct gaattaactt
   152881 cagtaaaagc tccaaaaccc tctgcggcat accacctgcg aaatagagca ggggaggaga
   152941 ccatgcctgc tataacccga tcttggccat tttcattcct atgcactaaa ccaatgagcg
   153001 tggcccagat tggaaggccc cttaaaaagt tctttgttcc atcaattggg tcgattaccc
   153061 aatatctatc caacggctgg atatcttctg cgctgccaaa ctcttctccg acaattttgt
   153121 catttggtcg ctgggctttg agaacctctc taattttctc ttcaactgct ctatcagcat
   153181 cggtaaccgg tgagagatct ggcttacttt caattttaag gtcttgagct aaaaatctag
   153241 atgatgagat ctcatcactt aaatcagcga gatatttaag taactcaata tctcctagaa
   153301 gatctcctgg ataggtcata gtccctagat tatctcttgg ttgcttgaat caagcagcct
   153361 ttgcaagctg gctaggcgcg aggatgcttg aggacttgtc tttacgtagt catttagagc
   153421 gcaagttgct tcatcatgag agcaattctt aggacagttt gcaataactg atgcgaattc
   153481 agtaaatgag gcgataactc ttgatttatc aatatgttct aggccaaagg atctaactcc
   153541 gggggtatca attatccagc ccccatttgg taattcaaaa gagatagcac ttgatgaggt
   153601 atggcgaccc ctaccagttg ttgaattaac atctccagtg tcgcgctttt cgattccaag
   153661 gagtgcattt acaagtgttg atttaccaac tcccgagtgg ccaattaaga ctgaaacttt
   153721 tgcctctaac gctttatgta gctgagttaa atcatcacca cgttttaact taaagtaatc
   153781 aacttccaaa tctttgtaag ctattaagaa ctcatcagga tctgctaaat ctgccttact
   153841 cataacaatt atcggtttga tgccttgatc aaatgccact gctaaacagc gatcaataaa
   153901 tccctgtctt ggagcaggat cagcagatgc tgcaacgata accatctgat caagattggc
   153961 tgcaattgtc ttttcaaagg ccccagcatc atcaatagtt ctacttaagc tattacgcct
   154021 gggttgcacg gcaacaattc gagccaggga tccagcatct ccacttacat ctcctacgat
   154081 atttactaga tctccaacca ctacagcgtt ctttcccagc tctctagcct tcattgcact
   154141 aactaatttc ccactagcaa tgcgacatag agttctgccg cgatcaactg ttattacctg
   154201 agccgcaatt gagtcactat gtgaaggtct atctttactt cgccggcgcg ttgatttagc
   154261 cgggcgaatt cgaatatcgc tctcatcggg gcggcgtcta accatttagt agttgactcc
   154321 aaagcgctgg aaaatcagtg attgtctttt tcgttgtttc aatattttct acttcaactc
   154381 catcgacggc aaggccaatg agcgcgcctg ctgttgcaag gcgatgatct tcataggtgt
   154441 gaaaaactcc agctttcata ggctttggaa gaattctaag tgcgctctcc tcttcaatga
   154501 cttcagctcc aagggcagtt agttcatttt ttaaagcgct caatcgatcc gtttcatgga
   154561 gtcttaaatg gccaataccg cgtaggtacg agggggagtt tgcaaagcaa gccagcgctg
   154621 caatcactgg ggttagctcg ccaacatcgc caagatcaat atcaattcct cttacctggc
   154681 catctgatct aactcttaaa ccttgatcta caaaatcaat tactccgccc atttctgaaa
   154741 atatctgcct taactgatct cctggttggg tagtttttct tggccaatct ttgataatca
   154801 cttcgccgcc acatactaaa ggcgcagcca taaagacagc agcattagaa agatctggct
   154861 caattactag atcaactcca gataatttag ttggcttaac gctccattga ttcgcaatag
   154921 aggtatcaac aactgctcca tattgggcga gcattgagat agtcatctcg atgtgaggct
   154981 ttgaaggaag gttcttacca acatttttaa tcttaagtcc ctgcttcatt aaaggtgcaa
   155041 ctaataagag cgaggagaga aattgacttg aagcgctggc atcaatttca acttctccac
   155101 catttatata gcctgcgcca tttataacca aaggcaagct atatctacct tgatgttcaa
   155161 tgcttactcc aagactttcg agcgccttga tgactgggcc tagagggcgc tggtgggaac
   155221 gcggatctcc atcaaaggag atcaatccct tggcaagtgc tgcaagaggc ggaagaaatc
   155281 tcattacagt tccggcattt ccaacatcaa ttcctgcagg gcccatcaag ggcgctggag
   155341 taattttcca aagatcttcc tcaatctcaa tctttacacc aaggctagat agcgctttga
   155401 tcattaaatc tgtgtctctt gaacgcaagc cccttctcaa agtggatggt gatgaggcaa
   155461 gtgcgcttaa caccagagct cgatttgtaa cagattttga ccctggaata tttatggcgc
   155521 ccgagattgg ctttgatgcc ctaaagggtg cgctccacat acttgaaagt ctagttcatc
   155581 gccataagta ttagattagg agtatgtgcg gaagatatgc cctatcggcg atgatcagcg
   155641 atattgctga agagttttct accaatagcg ctccaggcat cagccttccc gccgattgga
   155701 atattaagcc aaccaatgat gtctacatca ttaaaaatca agcaattgaa attgcctcct
   155761 gggggatgat tgcgcattgg tcaaaaagtg atgatgaggc aacaaaatct caatcaagtg
   155821 caatcaatgc aagatctgaa agtgttcatg agaaaccaac attcaaaagc gccttcagaa
   155881 gtaatcgttg tttacttcct gcaagcggtt actatgaatg ggcaagtgag cttggaaaat
   155941 acaagactaa gcaaccaata tatgtttcaa gagatgatga aaaattactt gcctttagtg
   156001 gaatctttca aagttggaca tctccttcag gaaaagtaat ccaatctgtt gcaatcatta
   156061 ctaggcaggc agtgggccaa ttagctcaag tgcatagccg gatgccagta tttctcccaa
   156121 aagagagatg gcaagattgg atgaatccca agattaatga tgtagacaag attagagctc
   156181 ttatggatgt tccaactcct gatgcaaatc ttcgctttca tccagtatca agtgcggtca
   156241 attcgattac tggcagcggg cctgggctta ttgagccaat tgaattagga gagcccgaga
   156301 ccctcttcta ggcagggaat aaagctctgg atatgcgggt taggctgcta tggcgataac
   156361 cttggattta atgccaaccg aaatttcaaa ccaaggctcc agggatgctg gagagctagt
   156421 tcgcgttgat cgcgaaaaag aaagtgcgga gcagagacga aagcgtttcg aagctgatgc
   156481 gctgcaatat atgaatcagc tttattcagc agcacttaga tacaccaaaa atccatcaga
   156541 tgcccaagac ttagttcaag acacctatgc caaagcctat gtttctttcc atcaatttga
   156601 gccagggact aacttaaaag cctggcttta tcgaatatta actacaactt ttatcaatac
   156661 ttatcgcaaa gatcagagaa gacctcagtt atcagatcaa gagttagagg attggcagat
   156721 agctgatgct tcatctcata ctagcgatca gggtaagtca gcagaagatg ttgttctaga
   156781 aaacctaccc gatagtgata tcaaacgtgc gcttgctgaa attcctgagg agtttcgaat
   156841 tgcagtttat ctagctgatg ttgaaggttt ttcttataaa gagattgctg aaattgttgg
   156901 tgttccaact ggaacagtta tgtcaagact tcatagaggc cgcaagcaac tacgagagaa
   156961 attaactgat tacgcccgtg aacttggcta tgtgaaggga ggcaagaaat gagcatccca
   157021 tgcgataagt gctgtgagag tttagttcta tttatcgata acgaaattga tgactccgct
   157081 acttttaatg aaattgcttt tcacttgcaa gattgccaag gatgtcgcgg tgagatggag
   157141 agccagagag ctgctcttaa tctgattcaa tcacttttaa ctagatcatg ttgtgagagt
   157201 gcgccttcta gtcttcaaga gcagattgct ctccgccttg ccgagcttgc gggccaagag
   157261 atgcttccca cttctcagag cgagattatt acccagtatc gccgaactga aatcacaatt
   157321 gatggtcaga cttcaataga gattgaaact tctcacgaaa tcaggcgcga ctttcccttt
   157381 tagttaaccc cgccctgcta agttaatttc tatgcaagca gagttaattc ttggagctgt
   157441 tggtatatcc cagatggtgg ttaggcctgg agataccgca cttgctcatg gcagtggaga
   157501 tatgccaata ctttcatcaa gttttcttat caccttgatg gaaagtgctt gcaattcagc
   157561 tattgctgag tttttggaaa atggtgaaac gaccacgcta actgatatgg aacttgaaat
   157621 tcatgatgct ctgggtattg gcagtgaaat tcaaggctct gcaacatgtg ttagctatga
   157681 agagggaatt cttacctttg cctgcgaaat tcacgatgga tctcggttga ttgctgcatc
   157741 agtaatgcgc cgcgcacttg ttgagagagt aacttttcta gctagaactg cagcgctctc
   157801 gctaatctcg gaaggtaagt agtagtaaaa ctagctggca aatacttctg caagaagtgc
   157861 tttctgttct tcctcgtgca tgtagatact tccagtagca gggcttgcag cagattttct
   157921 agaaactcta tttagctttc ttgccccaag tgcctcttga agattgcttg aaataaatgg
   157981 ccatggacct tgatttgctg gctcatcctg aacccaaact aacttcgcat taggcgcttt
   158041 tgaaagctct gccaatattt gatcgatagc aagtggataa agttgctcaa gcctaattat
   158101 cgctgtcgag ctatctgagc gttttgctcg ctctgctact aactcccaat aaatctttcc
   158161 gctacaaaga actatctttg aggcgctagt aattgcagga tcactgagaa ctggagtaaa
   158221 tgttccagtg gtgaaatctg tgagctgggc agcggcagcc tttagtcgaa gcatggactt
   158281 aggttcaaag acaattaacg gccttctggc agggtttttc acatgccagc gcaatagatg
   158341 gaaataagaa gcagcagaac ttggctgagc aatagtcata ttgttttcag ctgccagggt
   158401 taagaaacgt tcaattcggc cagatgagtg atctgggcct tgtccttcat aaccatgagg
   158461 aagaagcatg actagcgagg atctctcgcc ccacttttgt agtgatgagg agataaattc
   158521 atcaataatt gtttgtgcgc cattggcaaa atcaccaaat tgagcttccc aaagaactaa
   158581 agcatcctca cgctctagtg agtaaccata ttcaaagcca agggcggcat actctgaaag
   158641 caatgaatca atgacaaata gatgagtact tccacttatt aattctcgca gcggaatcca
   158701 ctctgatcca ttttcataat cacgaattac tgcatggcga ttagagaaag ttccacgacc
   158761 aacatcttga cctgcaagtc ttacggtgtg accctcgaga agtagagagc caagtgcaag
   158821 catctcaccg cctgaccaat caatagttcc atcatcaagc attgtgacgc gtcgctgtaa
   158881 ttggggagca agttttggat gtactttaaa tccttgtggc gttgctactt gagtagcagc
   158941 aatctttcta gccatctctt cactaattgc agtatttgcc tttagcggat ctaactcttc
   159001 tagtttatta aatgcaggtt tttctggaag tttgtcatgg accatattaa agattgcttc
   159061 tagttgaccc tggaattcct ttgcaatttc atcagactct tcttgggtga tatcgccgcg
   159121 tccaactagg gcttcggcat agagaactcg agttgagcgc ttagcatcaa ttaatttata
   159181 cattcttggt tgtgtaaagc tcggttcatc gccctcatta tgtccgcgcc ttctatagca
   159241 gaccatgtcg atgatgacat ctttattaaa gctctggcga tattcaaagg caactcttgc
   159301 aactcttaca catgcctcag gatcatcgcc attgacatga aataccggag cttgaatcat
   159361 cttggcaata tcggtgcaat agcgagaggt gcgagcagaa tctggtgagg tagtgaagcc
   159421 cacctgatta ttaatgacta catggatagt tccgccagtg cgataaccac gaagctgtga
   159481 catgtttagc gtctctgaga ccacgccctg tccagcaaat gcagcatcgc catgaatcaa
   159541 tactggaaga acgctataga tatctcttac attcttctta tcttgctttg ccctaacaat
   159601 tccttcaaga actgggttta ctgcctcaag atgtgaggga tttgctgcaa gatatatctt
   159661 ggtttgatcg ccagattcag ttatgaagtc cccatatgtt ccaaggtgat acttcacatc
   159721 tcccgagccc tggacttcat tttcttgata gtgcccttcg aattcttgga agatttgccc
   159781 ataatttttt ccagcgacat tagctaagac atttagcctg ccgcgatgcg gcattccgat
   159841 gcatacctca tctaattttg ctttagcggc atatcttgca attgcatcaa gaattggaat
   159901 aactgattcg ccgccctcca gcgagaagcg cttttgtccc acaaactttg tgtgaagaaa
   159961 tgtttcaaaa gcctctgcgg aattcaattt gcgaagaatt cttaactgct cctcccttgg
   160021 aaagaagggg gcgccaactt caacttggct ctgaatccat ttgcgctctt ctggtgagtc
   160081 gatatacata tactcaatac caattgatct gcagtaagag tcgcgcaaga ttcccagaac
   160141 atttcgcatt tttgcataag ggcgaccact aaagccgcca gttgcaatct ctctatcaag
   160201 atcccagagc gttaggccgt gagtaattac atcaagatct ggatggctac gttgctggta
   160261 ttccagtgga tctgtatcag ccattaagtg gccgctagtg cggtaggcct gaatcaattg
   160321 ctgtagacgt gctgctttat ttagctgctc tttgccctct ggaatatcgt tatgccaagt
   160381 aattggaaca taagggattc gaagagcagc aaatatttct tcatagaaat tatctgcccc
   160441 aagcaggatc tcatggattt tctttaagaa gtcgcctgat tgcgctcctt gaataatgcg
   160501 atggtcatag gtggaggtca gagtaattac tttagagatt gcaaggtttg cgatggtttc
   160561 ctcacttgcg ccttgaaact cggcagggta atccattgct ccaacgccaa gaatcaagcc
   160621 ctggccagtt actagcctag gcacagagtg aactgttccc agagtgccag gattggttag
   160681 cgaaactgtt gtgccactga aatcttcaac actaagtgct ccagagcgag ctttctttac
   160741 tagcgcttca taagaggccc agaagtttcc aaaatcaagg ccttcacatc ccttaattga
   160801 aggcacaagt aattggcgag atccatcttc tttagttaaa tcaatagcaa ttccaagatt
   160861 gatgtgatcg ggatatccaa ttgctggttt tccatcaagc tcggtaaaga aggtattcat
   160921 ctctggattt tcgcggagcg ccttaatcat ggcgtagcca attagatggg tgaatgaaac
   160981 ctttccacct ctggctcgtt ttaagtgatt attaattaca gtgcgattat caatcattaa
   161041 ttttgcagga attgctctga cactggttgc agttggaact gttagcgaag cttccatgct
   161101 tgtaacaact cgagcagaga ctcctctaag tggttcaact cttgcagcag ttggggttgc
   161161 aatttgtgga gcagattttt taactgcctc tgctggcgtt ggaattggag caggatttgc
   161221 tcgaacaact tcttgaatag gtggtggcgt attttgaatc tcttgcgcag gagcttgttt
   161281 aacctgttgg cgttttggaa ttggcggggt ggcggctttt gctactgagc ttgctgcgcc
   161341 attagttgct ggtttatatc cagagaagaa ctcttgccaa ttggcttcaa ctgaatttgg
   161401 atcggctagg tatcgctcat acatctcatc gacgagccat tcattagagc cgaatgagcc
   161461 gccaaattta ttctcaggag tcatgcctgc cacagaaagt aagcctatct ttgttgcgct
   161521 ctgccttcct accgccaagt ggcgttcttg gccacacttt ggcgatgcaa aaagcgcttc
   161581 tctagggcag gcttatgaaa tggcgttggc atttgttact ggggcaagca ggggtctggg
   161641 ctatgaggtg gcaaaggctc taaaagctcg aggcgatcaa gtaataggta taagtaaaga
   161701 tcgccagcgg gccgaggcgg catcaagtga gctcgggatt gcctttgagt taatggattg
   161761 ccagcagatt tctcaagtta gaagcgtaag ccaggagcta ctagccaaat atggaactcc
   161821 agagattcta gttcttgccc atggggtaat gagtgagaag atggcaaaga cgcttaagac
   161881 caatgatcaa gagtgggcaa gagttttgga tatcaatctc aactcagtct ttactttagt
   161941 aaattccata gctgccccaa tggctgaggc aagaaatgga cgagtaataa ttttctctgc
   162001 ctgtcttgga agaatgagcg gtcctggaaa tgcaggtgga cttgctcctt atcgcatttc
   162061 aaaggctgga gtaaatgcgc tagtaagaaa tcttgctcat gaaactaatc atggcgctag
   162121 aggattttta gttgatgcaa tatgtcctaa ccacagtaga actgatatgg gcggggctga
   162181 tgcgccaaga agcgctgccg aaggagcagc aactgcaatc tggttagcta ctagaacatt
   162241 taacccagga gatcagactg gattgctttg ggaggatcag gaagtagtgc cttggtgagg
   162301 gattgcagct agtgcggcaa gtaaagtaac tagagcaaag cttccaagac caattcctag
   162361 caaatctctc tcgccatcaa tcatgtaata agcaacaccg atggctatta aattagcaag
   162421 aacagttggt gcgcgggcga agtttcgctt tgcgataaat cctttgccag caaagaagag
   162481 tccagtgctg gcgatgctta agaaaataat ctcagccaat atggcatcga gctcttcgac
   162541 cttacttgta gcagttctta tcgcaaggta actagcaagg ccagcaagaa atagcgcttc
   162601 aaggcgcacc agagttgcag ctattagata gacctttgag ccaaagagct tcatatatgg
   162661 ccttaagaat aggggatgag atagctctag ttattatcaa aaaagctagt aaggcttggc
   162721 taattatgac ctcctttgat agcagctcat ttgatcggct aaccggccta atgaccccgg
   162781 tctatttcta tgaatcctta tctcgcctaa ggtcttgggc gcagagaagt gataaccctg
   162841 tgaccttaat tgctattaat ctaaaagatc taagtgatga tcaattgctg gaagtagcaa
   162901 gagagttaaa tagcgaactt aggggagggg atttacttgc ccgaatggct ccaaaaagat
   162961 tcttactcgc actcgttgct gatcaactag gagcaaggca atttttattt cgaatcacca
   163021 acaaaataaa ggaagctagt aattatcagc taattgagct ctctccaagc aaagatctag
   163081 cagaggcgct tagtgaaata gatatttgag atattactta tctgaaacaa atattgctga
   163141 gcggtaataa gcaagctctg caatggaatc ttgaatatca cctagggctc tatggttgcc
   163201 tgacttcttt ggagcttgga aataagcttt tggataccat ctctttgcta gctcctttat
   163261 tgatgagaca tcaatagttc tatagtggag aaagctattt aatagcggca tatccctagc
   163321 aataaagttt cgatccatcc caaccgagtt accagccagg ggagactttc cctctgctgt
   163381 gtattgcttt agatactcaa gaatcctctc ttcagctatc gagagactaa ctccatttgc
   163441 cactttttct attaatccag attcactatg cattctcttt acttcatcgc tcatcgaagc
   163501 tagtttctct ggagtcgttg cgataactag atcgattcca tctccaagca gatttagctc
   163561 gctatcggtt accaagactg cgatttcaat tagggcatct gaggctagat tcagcccagt
   163621 catctcacaa tcaatccaaa tcaaggcagg gttctcttgg gccacgcagg cacgataccc
   163681 ccaacttcta ggtcagagtt cacctcccgt tcacctagca cccattggtt atttacccag
   163741 agagggtcag attcactcac gccgaagccc ttcggcacct actttgattg aaaggttttc
   163801 ttaaatgttc tcaaagaagc gaatcctcgc aatgtttaca gcgacgggac ttgcactatc
   163861 tctaatgagc gcgccagcat ttgccaagga agcagatggt ccagtaacaa atcttggaaa
   163921 gtgcagctca cgaaataaga ttgcatatga tgctaaggga attgaatata agtgctccat
   163981 cgcaggtggt ggattaaagt ggaagaagac cggcggtaag caaggtgctg gcgcttcaac
   164041 ttcaacttca acaattgata caaagaaata tgcaaatgtt aaaggctcaa ttaaaattga
   164101 cggctcaagc acagttgcac cacttactgg tgtagcagct gaggcatttc agaaaatttc
   164161 acagactcag atcacagttg gtatttccgg aactggtggc ggacagactc gtttctgtaa
   164221 gggcgagcta gatatcgcaa atgcatcagc agcctattca gcaacacagc gtaagcaatg
   164281 cgaagatgct ggaattaagt tcaccgaact tcgcctagca actgatgcgc tatctgttgt
   164341 agtaaatcct aagaacacct gggcaaagtg cctaacagtt gctgagctaa agaagatttg
   164401 ggagccagga agcaaggtca attactggaa ccaagttcgt gctgatttcc caagagttaa
   164461 gatgccgcta tttggcgcgg gaacagattc aggaactttt gagtacttca cagagcagat
   164521 caatggtcgc cctgcaaagc gctcacgagt tgattacacc ccaacagagg atgacaacgt
   164581 cactgtaaat ggtgtcaagc gctcaactgg cgcaatgggc tactacggat tctcttacta
   164641 caaagaaaat accgatatca ataaggcaat tgcgattgat ggcggtaagg gatgcgcaga
   164701 accatcacta gagaatgtgc taagtggcgc ctacacccca cttgcccgtc cactatttat
   164761 ctatgtcaat aatgaccagg taaagaagaa tcctgcaata attccattcc ttgagttcta
   164821 cgcagcagat ctaaagaacc tctccgagaa ggcgaagttc ctacccctaa ctgctgagca
   164881 gaccaagaag ttggaagcag atcttgctga actaagaaag cttgccaact aaggtaaggt
   164941 tttattagta gcaaaaagct ttaaaaagtt ataacgaaga ggggtagagg atgagcgcca
   165001 cagcacaatc cggcagagcc ggaatgtcgc tagctccctc tacccacctt cgcagaagaa
   165061 atttaatcca attattaatc actagattta tggcttcagc cactttagtt tcagttctgg
   165121 tgactgttgg aattgttctc tccttgattc ccaccacatt tagacttctc aaggaaataa
   165181 ctacgcaaat atttggaacc gtctgggctc cgttatttac tccatctcaa tatggcatgg
   165241 cccctttatt aactggaact tttattatcg tttcaattgc catggtgatt gcaattccaa
   165301 tcggacttgc ctcagcaata tttctaagtg actatgcaac ttttaatctt cgtcgaatag
   165361 ttaaaccaat tcttgagatc ctggctggaa tcccaacagt tgttcttggt ctctttggcc
   165421 tttatgtagt aaacccagaa tttgtcgaga agtactggcc aattggtgag ccactggcat
   165481 ataccgcact tggcgcaggt ttgatgaccg gtattttaat tattccgatt attgcctcag
   165541 ttgctgatga tgcactctca tctgtaccaa gagctatgcg cgaggcggct tatgcattag
   165601 gtgcaacgag gcgagaggtt gccacgaaag tagttttaaa tgcaggactt tctggggtgg
   165661 tagctgcgct gattttggcc tttgctagag cctttggcga gaccaccatt gccctaatgg
   165721 ttgctggaag ttatccaaag ctttcggcaa atccaggcga tccaatgcag acgatggcat
   165781 cctttattgg ttttgctgga attggtgatg cagaagttgg ttcatctgcc tataacacaa
   165841 ttttcctagt tggaactgtc ttatttacag ttactttaat tctaaatatc ttgggaaata
   165901 gatttattgc aagatttaga gaggtttatg agtgatggca actcctagta cttcacttga
   165961 aagtcgcgta tcacttgatc gccgaattaa agattttact ttcactacat ttctttcgat
   166021 aagcatagtt gcctctcttg ctttcatttt ttatatcatc tatcgcctct ataacaatgc
   166081 tggattctta ctctcctggg atttggttag attctttcct tcctacgata ttgatactgc
   166141 aggttttcaa tcatcaatta tgggaagtat ctgggtagtt ggttttgcca ctttatttgc
   166201 agttcctatt ggggtgatga ctgctcttta tttagaagag tttgctggaa agcgaaatcg
   166261 ctttactgcg cttcttgagt taaacattca aaaccttgca ggtgttccag cggtggtctt
   166321 tggattgatc ggacttgcct tcattgctcg cggcccactt agttggggct ttaccgtggg
   166381 atcagctgcc atcgttcttg cgatgttgat tcttccggtg atcattatca tcactcgcga
   166441 agccattaga gcagttcctc cttcatataa agagggagcg ctagcacttg gcgctacgca
   166501 ttggcaggct gttagtaagt tagttatccc atctgccgtt cctggaactg caacgggaac
   166561 aattcttgcg gtctcccgcg caatcggtga atcagccccg ctacttcttc taggagctct
   166621 ctcctttgtc acctttaatc caactgggct agatagcggc tttaccatcc tgccgcttgc
   166681 gatctttaaa tacgcctcag actcacgcga ggaattccaa gttctagcct ctgctggaag
   166741 cttgattctt ctggcaattc tctttgctat gaacctattt gcaatcttgc ttcgcgatta
   166801 cactttgaga aagaggaata tttaatgtca atgattttta ggcaatctca ggcaaataaa
   166861 gtctctaagg aagaacctaa gaaagggtca agtccgatga acagcagcgc tactagaaaa
   166921 ccaagtgagc ctttgaagtt cgttgacccg gtcttcactc tcaaagatgt tgcggtctca
   166981 tatggcgact tcgttgcggt tagtgatgta aatatgcaga ttccatctca tgacatcacc
   167041 gcctttatcg gcccatctgg atgtggcaag tcaacacttc ttaggacttt gaatagaaca
   167101 aatgacctta tccctggcgc aaaagttcat ggctcactct tctatcatga tgcagatctc
   167161 tatcacccaa gtgctagcgc agttgaggtt agaaggcgca ttggtatggt ttttcagaaa
   167221 ccaaatccat
//
LOCUS       DAOROD010000002        96134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_6_length_96134_cov_31.355822, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000002 DAOROD010000000
VERSION     DAOROD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 96134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 96134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96134
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6_length_96134_cov_31.355822"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="speB"
                     /locus_tag="PLW53_00875"
     CDS             <1..967
                     /gene="speB"
                     /locus_tag="PLW53_00875"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRD36011.1"
                     /translation="FTFLGIPACDLDKPESYKGADVIIVGAPIDSGTSHRSGAKFGPQ
                     AIRMGDYLPHDGERPHLALRIDGLKELKIFDAGDLLMPPGNLEEALLVLKKATEKISR
                     AGAIPVVLGGDHSIASADVAGIAEHLGKGKVSMIHFDAHADTGDLQFGALVGHGTPMR
                     RLIDSGYVRGDRFLQLGLRGYWPDDVTLNWMKDKGMRSYEMTEIHHRGLTKVLDESFA
                     TLTKDCDGVFFSVDIDVVDPGMAPGTGTPEPGGMTSRELLEAVRRICLELPVVGIDVV
                     EVSPPFDSADITAILANRVVLEAVSAIAKRKRGDSYLPTQNLLDR"
     gene            complement(964..2304)
                     /gene="gabT"
                     /locus_tag="PLW53_00880"
     CDS             complement(964..2304)
                     /gene="gabT"
                     /locus_tag="PLW53_00880"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HRD36012.1"
                     /translation="MSSGPHLEQVRKLITPIPGPKSQELIKRRAEAVSASLGMAIPVV
                     VEKAGGGVIVDVDGNSIIDMGAGIAVVNVGNSADRVVKNVKEQVEAFTHTCFMIAPYM
                     GYIQVCEALNRLTPGDHKKKSALFNSGAEAVENAVKIARNFTKRQAVVVFEHGYHGRT
                     NLTMAMTAKNMPYKDGFGPFTPEVYRMPMSYPYRCDSCSGSCQESVLDTVLHKIEKEI
                     GAKNVACIVIEPILGEGGFIVPCKGFLPGLSKFCKENGILFIADEVQTGFARTGQMFA
                     CEDESIVPDLITTAKGIAGGLPLAAVTGRADVMDSIHASGLGGTFGGSPIACAAALGA
                     IATIEEENLVKRANELGLIMRNSLEAMKKKYPIIGDVRGRGAMQAIELVIPGSKDPNP
                     SALTSIIKYCQQNGVLILSAGTYSNVIRLLPPLVMPEHLLKEALEVLEAGLAQA"
     gene            2358..3494
                     /gene="dxr"
                     /locus_tag="PLW53_00885"
     CDS             2358..3494
                     /gene="dxr"
                     /locus_tag="PLW53_00885"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRD36013.1"
                     /translation="MRDIVILGSTGSIGVQALEVIAANPELFRVVGLSAGGNNLTLLA
                     KQVRDFNVPIVGVSSGAQELKSALSGVKVIDGTQASEQIASLTCDLVLNAITGSIGLG
                     PTLAALASGNKVALANKESLVAGGDLVMAYGSEKIIPVDSEHSALFQAMSAGRASDVS
                     KLILTASGGPFRDRKDLSKVKLADALAHPTWSMGPVVTINSATLVNKGLEIIEAHYLF
                     DIAYSKIEAVIHPQSIVHSLVEFKDGSTLAQASPPDMKGPIAYALAYPNRVAGAMAPI
                     NWSAKQSWDFEPIDEKRFPAVQLARRAGALGSSVAAIYNAANEVAVAAFMKDEIAFTA
                     IVDTIEKVVDKLSVSAPSRIRDISDVSAIEKDARMYAKEVLKKG"
     gene            3508..4689
                     /locus_tag="PLW53_00890"
     CDS             3508..4689
                     /locus_tag="PLW53_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRD36014.1"
                     /translation="MGFLGVIAFILALLISVMIHEYGHYITAKRYGMRVTEFFVGFGK
                     RLWSTQRGETEFGIKAIPAGGYCKISGMSPNEELADEFKPRAFYLASAPKKLVVLGAG
                     SFLHFVLALFLLFTLFAGIGTSQVLPTISQVLPCVPKESTCKAGDPISPAKRSGLMPG
                     DEILGVNGQELAWKESAEILRASAEREITLLIKRDGQVVNIAITPATRVVEGDSRGFL
                     GIINEFGLVRQNPIKAAASSVRATGAIFEGSIKALISLPSQIPSLIGQTFFGQERNSQ
                     GLVGIVGVARASGETVSSGNLSTGEKVATFILIVASLNIFVGIFNLLPILPLDGGHMA
                     VASYEAIRRRIAAMRGKADPGPVDVEKLTPITLVVFAVLLVLTVILLIADIFNPINLN
                     L"
     gene            4704..5855
                     /gene="ispG"
                     /locus_tag="PLW53_00895"
                     /gene_synonym="gcpE"
     CDS             4704..5855
                     /gene="ispG"
                     /locus_tag="PLW53_00895"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRD36015.1"
                     /translation="MVDLGIPSAPPPVLAPRRKSRQLKVGSVLVGGDAPVSVQSMCTT
                     LTADVNSTLQQIAELTASGCQIVRVAVPSQDDADALKDIARKSQIPVIADIHFQPKYI
                     FAAIDAGCAAVRVNPGNIKQFDDKVKEVAKAAGDAGIPIRIGVNAGSLDPRLLSKYGK
                     ATPEALVESALWEAGLFEEHGFRDIKISVKHHDPVTMIKAYRLLAAKCDYPLHLGVTE
                     AGPLFQGTIKSATAFAVLLAEGIGDTIRVSLSAPPVEEVKVGISILESLNLRQRKLEI
                     VSCPSCGRAQVDVYSLAEKVQAGLEGMTVPLRVAVMGCVVNGPGEAREADLGVASGNG
                     KGQIFVKGEVIKTVPEAQIVETLIEEAMRLAEEMEAAGVASGEPTVNVK"
     gene            5874..7598
                     /locus_tag="PLW53_00900"
     CDS             5874..7598
                     /locus_tag="PLW53_00900"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRD36016.1"
                     /translation="MLKMSSLFLRTLRDDPSDASVASHKLLVRAGYIRPIAAGIFSWL
                     PLGVIALRNVERVIRDEMDKAGFQEVHFPALLPKDPYEKSNRWEEYGPALFRLQDRKG
                     SDYLLGPTHEEMFTLMVKSEYSSYKDLPLSIYQIQTKYRDEARPRSGIIRGREFIMKD
                     SYSFDLDDAGLEVSYQKHRAAYIETFNRLGLKFNIVSAVSGAMGGSKSEEFLAPCSTG
                     EDTYVLCPKCGYAANVEAMVSKVSKKEIAKAPEMELLDTPDTPTIESLVAVMNQKYQA
                     NISASDTLKNILLVADGQVISILIPGDRQVDLKRVEANLAGVKELRLFEDADFAKNPA
                     LVKGYVGPQDAKKLGIKLYADPSVVVGSHWITGANTKNKHMRYVTHGRDFNVDGFIEA
                     VEINQGDPCPSCDSPIIIDRAIEIGHIFQLGRKYAQALDLTVLDSQGKSQVVTMGSYG
                     IGVTRALAAIAEQSHDEIGIIWPDEIAPAKVHIVATGRDDEPFIQAERIGKELEAKGI
                     SVMIDDRREASPGVKFKDAELIGNPYILIFGKSLAQGQVEFRIRRSGEKSEIALGEIT
                     SYLVKLLG"
     gene            7613..8389
                     /locus_tag="PLW53_00905"
     CDS             7613..8389
                     /locus_tag="PLW53_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRD36017.1"
                     /translation="MDISVEVILFLILAATFAGLVDSLAGGGGLIQLPALLVSLPDTS
                     PSVILGTNKVPSFLGTLGATASYLRKIKPDFKLAAVMGIPAFIGSAMGASVATKIPRD
                     AFKPIILFLLIVVAIYTYLRKDLGQVSSAKFAGRNRLLIGAAVGAVIGFYDGIFGPGT
                     GSFLMLALVAVLGFAFLEASVTAKLVNLATNLGAILVFGFNSQIIWALGLTMAAGNIA
                     GGFIGAKVGIRGGSVLIRRAFLAVTTALIIRLIYDTFLRG"
     gene            8451..8921
                     /gene="rimP"
                     /locus_tag="PLW53_00910"
     CDS             8451..8921
                     /gene="rimP"
                     /locus_tag="PLW53_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRD36018.1"
                     /translation="MALVDNLNELLAPVVKASGLLLEEIKVIPVGRSRIISVIVDHEE
                     RNLNLDEVAASSRAISEILENYSQLGDNPFTLEVTSPGVDRPLVKAYQWKKNLGRLIS
                     IVKVDGEKLIGRLKSLDLDSALLEVKSGEVSILFSEIKRATVEIEFNRKDGDKR"
     gene            8918..9913
                     /gene="nusA"
                     /locus_tag="PLW53_00915"
     CDS             8918..9913
                     /gene="nusA"
                     /locus_tag="PLW53_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRD36019.1"
                     /translation="MNVDMKVLEALTIERDIPLDRLITAIEIAVKSAYNEMEGAKPFA
                     HAKLDLVTGEIKILIPVFGPDGEKIDEISDEPEGFSRIASSTARKIIKEKMRESKDLE
                     IVGEFSATVGDIVSGVIQQGRDQKMVYIDLGRVEGKIPPHEQVPGEIYKHGDRIKSFV
                     VEVKQGQKGPEVLLSRSHPALVKQLFALEVPEIRDRIVEIMGIAREAGHRSKIAVRAH
                     RAGVSPKGALIGPMGARARAVMDELHGEKIDIVDWSDDPATYVGNALAPARVSSVEVV
                     NIDTRSAKVVVPDYQLSLAIGKDGQNARLAARLTGWRIDIHSDGATSGSEASEVS"
     gene            10084..12795
                     /gene="infB"
                     /locus_tag="PLW53_00920"
     CDS             10084..12795
                     /gene="infB"
                     /locus_tag="PLW53_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRD36020.1"
                     /translation="MAKVRVYELAKDVGMDSKDVLAKLQEMGEFVRSASSTVEAPVVR
                     KFLEKFPAVAATPKKAPAKKAPAKKAAAKKAAPVESVEQIDEITPPITPVIKESVEEK
                     PAPLPAKPPAAPAARVGNNPFATPSAPPRPPRPQGQGGTRPGNNPFGNPSAPPRPPRP
                     QGQGGAQRPGQVRPTGSRPPGAPARGGNMAGPRPGSVRPGFAARPQQRTDGSSRPQDR
                     NASSPYRSQGGQGQRPGGATGAPARPGAGKPGGGFAPGRPGQRGSAGGAFGKNAGKKK
                     NYKSKKTRREEIDNMQAPTLGGAIVPRGDGNTPVRLRRGASLADFAEKIGADPAGLVS
                     VLFHLGEMVTATQSVDEETLHVLGSEFGYKITVVSPEDEDKEILEQFDIDLDQELSDV
                     DPDDLSIRPPVVTVMGHVDHGKTRLLDAIRQTEVVKGEAGGITQHIGAYQIHHEHAGV
                     NRAITFIDTPGHEAFTAMRARGAKVTDIAVLVVAADDGVMPQTIEALNHAQAANVPIV
                     VAVNKVDKEGANPGRIRQQLTEYNLVAEEYGGDTLFVDVSAAKVTGIDQLVEAILLTA
                     DAAIDLRAIHEDDARGVAIEAHLDRGRGPVATVLVQRGTLSVGDAIVAGSAFGRVRAM
                     MDEFGQEVEVAGPSRPVQVLGFTSVPSAGDSFIVAPDDRTARQIAEKRQAGERHAALA
                     KARKKVSLEDFLEQSKVTTLNLILKGDVSGSVEALEDALVQLDVGSEVDLRVIHRGVG
                     AITKSDVTLASASGAVIVGFNVKPEAHTAIFADEEGVEVRFYSVIYKAIEEIEAALKG
                     LLKPEYEEAVLGRAEVREVFKSSKFGTIAGSLVSSGLIRRNAKARVLRDNVVIGDNLT
                     IESLKRFKDDATEVREGFECGIGVGNFKDIQIGDVFEAFEMREKKRA"
     gene            12799..13242
                     /gene="rbfA"
                     /locus_tag="PLW53_00925"
     CDS             12799..13242
                     /gene="rbfA"
                     /locus_tag="PLW53_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRD36021.1"
                     /translation="MGKSHRAAKVADRIKVVVASALESKVRDPRLGFVTITDVRVTGD
                     LQQASIFYTVLGDDEARLNTAKVLESAKGLLRTEVGRELGTRITPSLTFFADALGENA
                     KNFEDLLDQVRKSDEEIANLRSGAEPISGDDPYRQSRPKNYLSGE"
     gene            13243..14100
                     /gene="truB"
                     /locus_tag="PLW53_00930"
     CDS             13243..14100
                     /gene="truB"
                     /locus_tag="PLW53_00930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRD36022.1"
                     /translation="MILQDGFVIIDKPAGITSHDVVAKLRKTLGTKQVGHAGTLDPMA
                     TGVLVLGVNKATKFLQYITQGKKAYHAQIRLGQATSSDDAQGEITSTSDTSNVSDQEI
                     RDFLATQIGSIMQVPTKVSAIKINGQRAYDLVREGKEVDIPARQIDIYNLDVIEIKRG
                     EFLDIEINVECSAGTYIRSIARDLGSSLGVGGHLISLRRSLVAPFSLSDCSSLESPEI
                     RPLASEISKVMSVRNVDLLEVKELSFGRSLSASNSDGPVVAIAPDGKVAAILENREHG
                     AQPVAVFIS"
     gene            14114..15022
                     /locus_tag="PLW53_00935"
     CDS             14114..15022
                     /locus_tag="PLW53_00935"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRD36023.1"
                     /translation="MSSAEVTWHGAPLLGSALAIGIFDGVHIGHQVILQEGVRENLPV
                     VALTFDPHPTSVFAPDRVPTRLLTLNNRIAQLSMHGASSVAVIKFSSQFSQLTPDQFI
                     NQILKPIFDPALVLVGENFTFGAKAAGNVEYLKANSPFKVKAIPLYSHGNIKVSSTRI
                     REAIKAGNIEIANQLLTRPHQLVGPVIHGEKRGRTIGYPTANIGLDSYATIPSDGVYA
                     GWLSVGSHRWSAAISIGTNPTFDGVRGRQVEAYALDRDDLDLYDQEAKVEFGWRLRDT
                     IKFGSLDELLVQMKIDCDEARKLTSL"
     gene            15148..15417
                     /gene="rpsO"
                     /locus_tag="PLW53_00940"
     CDS             15148..15417
                     /gene="rpsO"
                     /locus_tag="PLW53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRD36024.1"
                     /translation="MALKPAEKKEIITKYGASASDTGSPESQVALLSRRIEEVTEHLQ
                     TNQHDHHNRRGLLLLVGRRRRILQYLAKTDIARYRAIIDKLGIRR"
     gene            15569..17776
                     /locus_tag="PLW53_00945"
     CDS             15569..17776
                     /locus_tag="PLW53_00945"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRD36025.1"
                     /translation="MEGQDVKSAAVTIDNGKFGKREIRFETGRLARQAAGTAVVYLDD
                     ETMLLSATTASKSPKDQFDFFPLTVDVEERMYAVGKIPGSFFRREGRPSEDAILTCRL
                     IDRPLRPSFIKGLRNEVQIVVTVMALDQNHMYDVIAINAASMSTQLAGLPFSGPIGGV
                     RIALIDGQWVAFPNHSDLENAVFDMVVAGRIAGDDVAIMMVEAEATVKTIDLIGSGAS
                     APTEEIVGQGLEASKPFIRQLCQAQIELAKVAAKPTAEFPVFLDYQDDAFAAVEKAAK
                     KELDAALKISGKQERETEIERLSTEVKQSLAESFVGREKEIPAAFRSLTKKLVRQRVL
                     REKIRIDGRGLRDIRPLIAEVEVVPRVHGSAIFERGETQILGITTLNMLKMEQQLDTL
                     NPEDHKRYMHNYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPGRQEFPYAIR
                     QVSEALGSNGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLISDDVDGKVEYVALTD
                     ILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPASVLSAALLQAKEARLAILKVMN
                     EAIDRPDDMNPLAPRIISIKIPVDQIGAVIGPKGKVINQIQEETGAEISIEDDGTIFI
                     GATNGTAADAAKAAILSIADPKLPEIGERYNGTIVKLATFGAFVNLLPGKDGLLHISQ
                     IRKMHGGKRIENLEDVMNVGDKVEVEIGEIDPKGKLSLIPVLDESQKSDTASE"
     gene            17787..19064
                     /locus_tag="PLW53_00950"
     CDS             17787..19064
                     /locus_tag="PLW53_00950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD36026.1"
                     /translation="MTVRRTVLASGLRIVTEEIPTVRSAAFGIWVNVGSRDESLKVAG
                     ASHFLEHLLFKGTKRRSALEISSSIEAVGGEMNAFTSKEYTCFYARVIDKDLPLAIDV
                     ISDLITSSLGKSSDVEAERKVVLEEISMRDDDPADLVHELFAETFYGDTTLGRSILGT
                     TKSIKSLSRSAVFNYYKKRYLPEDIVVAVAGNIRHQQVVDQIIAALSRDEFLDRTEKK
                     FNLRPSQEFSRKPKRNVGLINRKTEQAHIVLGMPGVNRDDKRRFAMGVLSAALGGGMS
                     SRLFQEIREKRGLAYSVYSYAQQFAGSGFLALYAGSTPSKAVEVIKIMRGVLEDVASN
                     GLTSEELIKAQGAVSGSLVLGQEDTGSRMSRIGKSELIYGQVLSFDEILREISAVDSA
                     AIAKLASEVLGSAPSLAIVGPFAKRSIFEKAMK"
     gene            19079..19825
                     /gene="dapB"
                     /locus_tag="PLW53_00955"
     CDS             19079..19825
                     /gene="dapB"
                     /locus_tag="PLW53_00955"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRD36027.1"
                     /translation="MKIKVAILGAKGRMGSESVKAISAADDLELVASLDLGDSLDLLK
                     SSGAEVVLEFTHPDSVMKNLEFAISNGIHVVVGTTGFDSKRIEEVKALLAKNPQVGAI
                     IAPNFGLGAVLMMQFAAKAATYFESVEIIELHHPEKADAPSGTATRTAELISDARAKA
                     KKSAMPDKTSSGLDGARGAKVGDVPIHSVRLRGLVAHQEVLLGDQGETLSIRHDSIDR
                     SGFMPGVLLAIRSVSTRPGLTFGLENLMDI"
     gene            19835..20089
                     /locus_tag="PLW53_00960"
     CDS             19835..20089
                     /locus_tag="PLW53_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRD36028.1"
                     /translation="MSTSITMYSADWCGDCVRSKRLLDSLQVSYEIIDVESIPGASEK
                     VIEINGGKRSIPVILFPDGTHLTEPSDIDLKAKLEALKII"
     gene            complement(20086..20391)
                     /locus_tag="PLW53_00965"
     CDS             complement(20086..20391)
                     /locus_tag="PLW53_00965"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRD36029.1"
                     /translation="MSQIWIATLVSSLICFLLKYLGYRIPESLLNRPAIQRINTLIPI
                     ALLSSLVAVQALTIEQQVVIDHRLVGLLVAAIALSFKANFPIMMLLAAASSALVYNL"
     gene            complement(20388..21014)
                     /locus_tag="PLW53_00970"
     CDS             complement(20388..21014)
                     /locus_tag="PLW53_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRD36030.1"
                     /translation="MAIPVGTYGAAFGAASIAAGFSILQTCLLSLLLFSGASQFAVVG
                     VMGSGGSAISAIATGSLLGFRNGLYGLKMAPLLRLRGFKRLIGAQLTIDESTGVAISQ
                     EGRGDEAARYGFWATGIGVYFFWNLFTLLGAIGAGAIGDPAAWGLDAVVPAAFLGLLW
                     PRLTDNKTRVLAISAALLALLLVPFAPAGVPIIATALLAVIFGSRAAR"
     gene            21132..21863
                     /gene="thyX"
                     /locus_tag="PLW53_00975"
     CDS             21132..21863
                     /gene="thyX"
                     /locus_tag="PLW53_00975"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRD36031.1"
                     /translation="MSQPSHPDIVFRDDVTVELVKSSASDADVIWAARVSTAGENSLE
                     EVNADPARSTGLINYLARERHGSPFEHTSMTFFISAPIFVFREFMRHRIASYNEESGR
                     YRELLPVFYVPNKARKLVQTGKTGHYVFVDGSDEQYEISVAAMKESYELCYLNYKKML
                     DAGVAKEVARAVLPVALYSSMYVTMNARALMNFLSLRTAREGSHFPSYPQREIEMVAE
                     KMEEHFARLMPITYAAFEKSGRVAP"
     gene            21887..22777
                     /gene="dapA"
                     /locus_tag="PLW53_00980"
     CDS             21887..22777
                     /gene="dapA"
                     /locus_tag="PLW53_00980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRD36032.1"
                     /translation="MSIQAPFGRLLTAMVTPFKDDLSIDWASVEKVAAHLVSTGHDGI
                     VLNGTTGEAPTTSDEEKVEIIKVVRKVVGSNIKIVAGAGNNETSHSLEQARQAAKAGA
                     DGLLVVTPYYNKPPQAGIAAHFEAMAGATDLPIMLYDIPHRTGVAIEPDTIVKLAANP
                     KIAALKDAKGDIASTSWVIKRCAIPVYSGDDILNLPLLSVGAVGFVSVCGHSVGSELK
                     QMLNAFFSGDVAKALEIHQRLLPVYTGTFRTQGAILTKAALDLMGLSGGRVRLPLVDA
                     TKAQIDQLREDLRAGGVKVK"
     gene            22777..24459
                     /locus_tag="PLW53_00985"
     CDS             22777..24459
                     /locus_tag="PLW53_00985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRD36033.1"
                     /translation="MNHPHPDLGLPPKLAAKTLRIVALGGLGEIGRNMTVFEYEGQLL
                     IVDCGVLFPSETQPGVDLILPDFSYLKERMADIAAVFLTHGHEDHITALGYLLRERED
                     IQVIGSALTLAFLKGKLRDHRISPIMVEVKEGHSRKVGPFDLEFIAVNHSIPDALAVA
                     IKTPAGVVLHTGDFKMDQLPLDGRVTDLARFASLGQSGVDLFMVDSTNADIPGFTTSE
                     REIMPVLERVIDGTKARVIVASFASHVHRIQQVIDIAYSKGRKVAFVGRSMIRNMTLA
                     AEMGYLHIPPNTLLEADEIESAGDKVVLICTGSQGEPLAALSRMANGDHQIRVGRDDT
                     VILASSLIPGNENSVYRVINELTRFGARVVHKGNALVHVSGHAAAGELLYCYNVVKPK
                     NVMPVHGEWRHMTANAELAISTGVKPSNVVVVDNGVIVDLSEGRAKVVGAVPVGYVYV
                     DGQSIGDITEASIKDRLILGEEGFISVIVVIDSQSGKIVAGPDIHARGFAEDLELFNE
                     VKGRIERALTGAVAGGINGTHQLSQVVRKTVGSWVGEEHRRRPMIVPVVIEV"
     gene            24509..26842
                     /locus_tag="PLW53_00990"
     CDS             24509..26842
                     /locus_tag="PLW53_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRD36034.1"
                     /translation="MAKKKSARKASGLLSAIATGLGFIWSAIAKALGASIRFITRGAK
                     DLEPEHRRDGIGLILLIVALIAAATSWMQLDNAFGRAAYAFFYGGFGRVAILTPILFG
                     YFAFRLFRTPQDKGATGRITIGSLLLVLSSTGLIHILNPSSINTGATAMREGGGWIGY
                     GVSTPLVALLTDMLAIPILILTLIFGALVITATPFSKVIDLFKRLISGASNKVSGALD
                     QRRERKLEREEFEIADTPPFETPLVQDFSNAQEEDLEEEIDEESFDEEFTVEIPKPEV
                     SAKPARPVQLLLSSTDSYELPSMDLLRTTPSSKTKTKANDQIVQSLTEVFAQFEIDCE
                     VTGFMRGPTVTRYEVELGSGVKVEAITALSKNIAYAVASSDVRILSPIPGKSAVGIEI
                     PNSDREIVSLGDVLRSSVAGNDHHPMVIALGKDVEGSFICANLAKMPHLLVAGATGAG
                     KSSMINSLVTSVLMRATPNDVRLILIDPKRVELNSYEGIPHLIAPIITNPKKAAEALA
                     WVVREMDMRYDDLSTYGFRHIDDFNKAVRAGKVIAKEGADAALEPYPYLLVVIDELAD
                     LMMVAARDVEDCIVRITQLARAAGIHLVIATQRPSVDIVTGLIKANVPSRLSFATSSL
                     ADSRVILDTPGAEKLVGQGDALFLPMGASKPMRIQGAYVSEREIEAVVSHVKSQLKPV
                     YREDILKPVVATRGSDEEIGDDKELLIQAIELVVSTQFGSTSMLQRKLRIGFAKAGRL
                     MDLMESRGIVGPSEGSKARVVLVKQEDLASVLEVIQG"
     gene            26964..27680
                     /locus_tag="PLW53_00995"
     CDS             26964..27680
                     /locus_tag="PLW53_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRD36035.1"
                     /translation="MSVGSTLKEMRKSAGFTVEQLAKRSRIPASVIEDLEKDNFLTAG
                     GPTYARGHIKTIARICGVGDSDVLIKFESQTIPLSKSLRDLLNENSVTKQKKERKPIS
                     WKTLTGAAAGLFIFALVGTAIISIEDSPEQSGITASSNGKDESQPSASIRDGVEVRLK
                     AVNGLSWVAVSDSTGTTQFSGRIRQGEERVFSDNQLLYLVIGNAGAINLTVNGEDIGV
                     AGAVGEVLRLEFGPQASNQG"
     gene            27710..29110
                     /gene="rimO"
                     /locus_tag="PLW53_01000"
     CDS             27710..29110
                     /gene="rimO"
                     /locus_tag="PLW53_01000"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRD36036.1"
                     /translation="MSKPKSVALVTLGCARNDVDSEELAGRLAADGWQLVDDVATADV
                     ALVNTCGFIESAKKDSVDALIEAHSMKADGTLRAVVAVGCMAERYGKELAEAMPEADA
                     ILSFDDYADISSRLQNILAGNSKPAHTPKDRRSLIPISPVARQEMKVELNTKMGQGGT
                     FARKRLANSPMAPLKIASGCDRRCSFCAIPQFRGSFVSRTPDEIITEARWLAENGVSE
                     LFLVSENTTSYGKDLGDLKAMEKLLPALSKIDGIERIRASYLQPAEIRPTLLQALIAT
                     EKVVPYFDISFQHASGTLLRRMRRFGDGEKFLHLLTQIRALSPEAGIRSNFIVGFPGE
                     SQGEYQELVDFVNSSNLDAIGIFAYSDEDKTEALTLDGKLDPQVISERTSELTMIAEE
                     LVMQRATDRIGQRVRVLIEDEELQEGRAEHQGPEVDSSTFVTIAGRPSAGYKVGQYVD
                     AIITDTSGADLIAQSL"
     gene            29107..29637
                     /gene="pgsA"
                     /locus_tag="PLW53_01005"
     CDS             29107..29637
                     /gene="pgsA"
                     /locus_tag="PLW53_01005"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRD36037.1"
                     /translation="MNLPNALTIARIAALPFCAWALFKNGGDDPNWQIIAWFSFFIVG
                     MTDVLDGIIARRWNQVSSFGIILDPIADKAFIATALIGLSILGKMPWWVTVLILTREV
                     GITVLRLLVIKKKVISASKGGKIKTLLQNFSVGFYILPLPEALHTPRDILLAIAIALT
                     FITGYQYLKDVVKSKP"
     gene            29634..30104
                     /locus_tag="PLW53_01010"
     CDS             29634..30104
                     /locus_tag="PLW53_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRD36038.1"
                     /translation="MSDLAATVIDLLRKRSESISCAESITGGAITSKLVSVAGASDVL
                     LGSIVAYSKEIKMSQLGLSAELIDSKGLVSQEVAIEMAKGARQRLGSNWAISSTGSAG
                     PTALDGSTPGEIWIAILGPDRQESVKLSLNGARQEVINGAVESALTLLERILRS"
     gene            30144..30416
                     /locus_tag="PLW53_01015"
     CDS             30144..30416
                     /locus_tag="PLW53_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD36039.1"
                     /translation="MELLRSHIGQCLRTERISQSRTLRDVAKAARVSLGYLSEVERGQ
                     KEASSELLNAICIALDLSLSKVLVEVTAQVKLNETPVLSVVDTNAA"
     gene            30510..30971
                     /locus_tag="PLW53_01020"
     CDS             30510..30971
                     /locus_tag="PLW53_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD36040.1"
                     /translation="MAAAKELLAQSGISGTNMIEIADRAQVSRASLYNHFRDKHEVFL
                     ALVESELERISTLAMIAQSRSEALYLISCEISNHPGLKSALASDGEIMANALTAREHK
                     IWVEIYAQLSKIFATDVVGVGLILRWLMGQVTAPLSDEHSKEQAERLASIL"
     gene            30988..31185
                     /locus_tag="PLW53_01025"
     CDS             30988..31185
                     /locus_tag="PLW53_01025"
                     /inference="COORDINATES: protein motif:HMM:NF022693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HRD36041.1"
                     /translation="MRITELRRRLTAHFGPDWAPSYSKDFVMAELGGQSVDQALEMGF
                     DPADIWRAVVKNNPDISSELK"
     gene            complement(31178..32620)
                     /locus_tag="PLW53_01030"
     CDS             complement(31178..32620)
                     /locus_tag="PLW53_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD36042.1"
                     /translation="MTFKTREPYLGAMVDLAPFQSPRPAEYAPMPKPLKRRDLKGLNI
                     PGGPRSPLLTLRFQKDTPAFLLNAKAEYGDCSSFFLAGQLFITAFSPEMVNEVTVSKQ
                     GSFIKGVGFDRMRKVLGSGLLTNEEPIHLRHRRLMQSPFHISKISTYAQTMLNLTRKH
                     ISNWSDGQVVAIGPEMMSLTFDIVADILFGTDISADTKRVQDSMHIAIDRIERTMLPG
                     LDRLDNLPIPYFRKFEKAANELHNVATRIVTERIASGVKRDDLMGVLLEAETVDGEKL
                     SAREISDETLTLILSGHETTANVLTWTFAYLSQNPKYWDGLAKEAEEVFAREGDEDFA
                     RVILAAPLSGAIFSEVLRLAPPVWISPRRALEDVTLGGREIPKGAHVLVSQYVTQRDE
                     KYFPSPNEFIPERWSNDFEKSLPRGAYFPFLAGSRKCLGDQFALLEGRIIILEIARRA
                     RLSLTGGFPKAQPRATYRPKGAVAMRYELI"
     gene            32774..33859
                     /gene="recA"
                     /locus_tag="PLW53_01035"
     CDS             32774..33859
                     /gene="recA"
                     /locus_tag="PLW53_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRD36043.1"
                     /translation="MAKEAKDPNEKAKPSAERQKALDTALAQIERQFGKGSVMKMSDR
                     TATPIEVIPTGSIALDMALGIGGIPRGRVVEIFGPESSGKTTLTLHAIANAQKNGGIA
                     AFIDAEHAFDSEYAKKLGVDIDALLVSQPDTGEQALEIMDMLVRSGALDLIVVDSVAA
                     LVPRAEIEGEMGDSHMGLQARLMSQALRKITGALSQTKTTAIFINQLRDKIGVFFGSP
                     ETTTGGKALKFYASVRMDIRRIETLKDGQEAVGNRTRVKVVKNKMAPPFKQAEFDIIY
                     GIGISREGSLLDMGVDLGIVKKSGAWFTYEGDQLGQGKENSRQFLLDNPALANEIEGK
                     IRAHFVASDIDPALVAAIDEAAADVEF"
     gene            33846..34343
                     /locus_tag="PLW53_01040"
     CDS             33846..34343
                     /locus_tag="PLW53_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRD36044.1"
                     /translation="MSSSNSVEVESDPYQVAQTIALMALGRRAKSRGELFKLLKSRGI
                     EEEVANAVLFRLAEQGFINDHEFARYWSESRQRTKKVSKRIISGELRSKGISPEIIEW
                     ITSDISDDEEFANAMTFAERKARAVTSLAPEVAYRRLHGALSRRGFSGHVVSRVLAEL
                     GISRS"
     gene            34378..35799
                     /gene="miaB"
                     /locus_tag="PLW53_01045"
     CDS             34378..35799
                     /gene="miaB"
                     /locus_tag="PLW53_01045"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRD36045.1"
                     /translation="MSRTYVIQTLGCQMNVHDSERIAGSLDAAGYVPAADGSTPDLIV
                     FNTCAVRENADNKLYGHLSFLAPEKKLRPELQIAIGGCLAQKDKSVILEKAPYVDVVF
                     GTHNMGSLPALLERARVEKRAQIEIKESLEHFPSTLPARRHSPFSAWVSISVGCNNTC
                     TFCIVPALRGREKDRSSAEILSEIRALVADGVIEITLLGQNVNAYGVDFGDRGAFAKL
                     LRACGEIEGLERVRFMSPHPRDFTDDVIQAMAETKNVMPHLHMPLQSGSDAILAAMRR
                     SYRVDRYRSIISKVRSALPNATITTDVIVGFPGESEADFQATLDLCSEVGFLAAYSFK
                     YSKRPGTPAAVMDNQIPEQIVAQRYDRLHQQINQIAARVNNEVLGKRVEVLITDIDGS
                     SATGKSRDFRLVHFEAPATARPGDIAELTIKESKAHFILGDSQSVSIRPTRGGDAFSA
                     RKAEAETKGVALGMPTLKSVHSK"
     gene            35796..36707
                     /gene="miaA"
                     /locus_tag="PLW53_01050"
     CDS             35796..36707
                     /gene="miaA"
                     /locus_tag="PLW53_01050"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRD36046.1"
                     /translation="MSVIVICGATATGKSDLALSLAEAIGGEIVNADSMQLYKGMDIG
                     TAKLPLSKRRGIPHHLLDVLRVNQEASVAQYQIDARNIIDQLLELNKPAIVVGGTGLY
                     IKAILDDLNFPDTDPALREKIAKQGQELGQDVMHQRLAKLDPAAAAAIPKENLRRVVR
                     ALEVIELTGKPYTANLPRAGSSKYPHAKQFGLVMERSTLASRIDARVEKMWDEGLVDE
                     VKGLISQGLLEARTAQAALGYAQVIKFAKGELTQSEAKEETKRATRQYARRQETWFSR
                     DERITWIKGGSRQQMLEEIISSTISPR"
     gene            36704..37510
                     /gene="dapF"
                     /locus_tag="PLW53_01055"
     CDS             36704..37510
                     /gene="dapF"
                     /locus_tag="PLW53_01055"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRD36047.1"
                     /translation="MKAPLTATYGHGTENDFVILFDPDDKNNLSSKQTAAICNRASGI
                     GADGLIRIIKRDGKWFMDYRNADGSLAEMCGNGIRVMARYLVDRGHQGAGIFSILTRD
                     GAKYLSADVAGDISVNMGQVEVIDGEITAANNGRVWSGYNLNIGNPHAVVFVDSLDDV
                     GDLKDPPVVRPKEEYPEGVNVEFVQFLENGELAMRVHERGSGETRSCGTGTCAVALAA
                     TLKKGMKLPAKWVINPPGGRLVVEIDPHSNATLTGPAVLLKDFDLEPYLG"
     gene            37503..38948
                     /gene="hflX"
                     /locus_tag="PLW53_01060"
     CDS             37503..38948
                     /gene="hflX"
                     /locus_tag="PLW53_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRD36048.1"
                     /translation="MAKKKELPEIEDLISESQQVAAEFDSEIFIPDSYQSTQQDLSDR
                     QALRRVIGLSTELSDISEAEYRQLRLERVILVGVWTEGSVKDAENSMEELAALAETAG
                     STVLDALIQRRDKPDPATFIGSGKVSELKAIVKSTGADTVVCDGELSPSQLRNLEDKV
                     KVKVVDRTALILDIFAQHAKSKEGKAQVELAQMSYMLPRLRGWGDSLSRQAGGIGGRG
                     PGETKIEVDRRRIRDKMSKLRREIGDMKVARDTKRQERKRNSIPSVAIAGYTNAGKSS
                     LLNRLTNAGVLVQNALFATLDPTVRRSETSDGRTYTLTDTVGFVKHLPHDLIDAFKST
                     LEEVSGADVIVHVVDGSHPDPLGQIKAVRGVIADIGGAKIPEIIALNKADIADPEVIA
                     QVLIQEPDAYLISVHSGVGIEKLIRAIESSLPRPRIEVRTVLPYDRGDLVSQIHEHGE
                     ILSQEYLPEGTSLHAMVDGSLAHALEKYSVN"
     gene            complement(38952..39623)
                     /gene="lexA"
                     /locus_tag="PLW53_01065"
     CDS             complement(38952..39623)
                     /gene="lexA"
                     /locus_tag="PLW53_01065"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRD36049.1"
                     /translation="MSKNPSKLTELPDGPVDENGLTPRQTKILLAIKEAMETNGYPPS
                     MREIGEAAGLSSPSSVKYQLEALESKGWIRRDPSRGRALEVLTPGDERFEDIKPERTH
                     NVPLVGRIAAGGPILAEQNVEELFPLPASLVGEGDLFMLKVVGDSMINAAICDGDYVV
                     IRSQKDANKGEIVAAMIDGEATVKTLSKKNGQIWLLPANDSYQPIDGNQAEILGVVTA
                     VLRSL"
     gene            39783..40166
                     /locus_tag="PLW53_01070"
     CDS             39783..40166
                     /locus_tag="PLW53_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRD36050.1"
                     /translation="MTTIAINPFLAKDARRVKTPINQGPAALRLTPRGRGLARLAVIS
                     SLSILLLSGFAAFNGATAGSNSESISSPYVKISVKPGDTLWSIAEGIAPQADRRALVA
                     DIVEVNNLKSPNLQAGQKIYIPTRP"
     gene            40331..40831
                     /gene="nrdR"
                     /locus_tag="PLW53_01075"
     CDS             40331..40831
                     /gene="nrdR"
                     /locus_tag="PLW53_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRD36051.1"
                     /translation="MNCPFCRFEDSRVIDSRPSDEGSSIRRRRECTQCGSRFSTSETA
                     VLSIIKRSGATEPFSREKVINGVRKACQNRPVDDDDLALLAMRVEEALRSTGQAEIEA
                     QEVGMAILPPLRELDEVAYLRYASVYRNFSSLEDFEGEIALLRALPIEKSEIESESQV
                     ASPQLK"
     gene            40934..43744
                     /locus_tag="PLW53_01080"
     CDS             40934..43744
                     /locus_tag="PLW53_01080"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRD36052.1"
                     /translation="MSDTVINRPSGKAKSEKGLSMERIYTTAGQHPYEGVNWERRDVV
                     QTNWKSGEVVFEQRGVEYPDFWSVNASTIVTTKYFRGAVGAENREHSLKQVIDRVVLT
                     YTKAGKEHGYFATPEDAEIFEHELTYMLLHQVFSFNSPVWFNVGTSAPQQVSACFILS
                     VDDTMESILNWYREEGMIFKGGSGAGLNLSRIRSSKELLKSGGTASGPVSFMRGADSS
                     AGTIKSGGATRRAAKMVVLDIDHPDIEEFIETKVREEDKIRALRDAGFDMDLGGKDII
                     SVQYQNANNSVRVNDEFMRAYESGAKFGLTARATGEVIEEVDSRQLFRKMAEAAWACA
                     DPGIQYDTTINDWHTSPETGRINASNPCSEYMSLDNSSCNLASLNLMKFLKSDGSFDA
                     KNFVKAVEMIITAMDISICFADFPTEAIGVTTRAYRQLGIGYANLGALLMASGLAYDS
                     EGGRALAGAITSLMTGTSYRRSAELAGIVGPYEGYARNAAPHTRVMKKHQAASLSAKS
                     VSTLDRDVLAEANKQWDSCIALGEKNGWRNAQASVLAPTGTIGLMMDCDTTGIEPDFS
                     LVKFKKMVGGGSLQIVNQTVPQALKKLGYTDETIEAIVEYIAANGHVVDAPGLKVEHY
                     DVFDCALGTRSIAPMGHVRMMAACQPFLSGAISKTVNLPADASVEDVEEVYYEGWKLG
                     LKALAVYRDSCKVGQPLSDAKSKNNGKDDQVSAAPAPATRKRLPKSRPSRTTSFSVGG
                     AEGYMTAGTYEDGQLGEIFLKLGKQGSTLAGVMDVFSIAVSIGLQYGVPLETYVSKFT
                     NSRFEPAGITDDPDIRMAQSMMDYIFRRLALDYLPYEERAAFGIYTAAERAAALETGE
                     YAQIVEEKIEVVEEKKASAPVAKPAKAETSLHQIGSSTELLEKIAGVKSDAPLCVTCG
                     VKMRASGACYVCEGCGNTSGCS"
     gene            43892..45898
                     /locus_tag="PLW53_01085"
     CDS             43892..45898
                     /locus_tag="PLW53_01085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRD36053.1"
                     /translation="MSAESVRKALAAAVEAIGGSQRAGQIAMAEAVANALEDRHHLLV
                     QAGTGTGKSLAYLVPALVHGKRVLVATATLALQRQLIERDLPKVQKALEKELGRSITF
                     AVYKGVGNYLCLQKMNADDSGVDSQVLVEISSLESDARRLRQWASRDGISGDRDDAPE
                     VDRRVWLANSTSGRECVGADECRFGAQCFAVKAKTKAQSADIVVTNHTLLAIEIVDSH
                     PILPERDAVILDEAHEFMDRTTQAVTEELTAARASRAANMLRKHLPGKAAEALGKACD
                     GFSRALDEYGQEFRTKSGDERKLQELPLLLAPWVRKIKEAAESAIALINADSLIVDPD
                     ALAERARVKGAVNEIKTTASKMLKIGSNQVLWFEPTFSTLYLAPLEVSHLLRENLFTK
                     TPVIATSATLSVGNSFAAIAKSFGIDPLEASQDESSESGGDIDPENLVSLDVGSPFDF
                     ASQGALYLPRDLPEPTRDGPSPQALVELGELIDAAGGRTLALFSSWRGVEMADEHLRR
                     VLAELPIKIITQRRGDSVGVLVEKFASDPTSILLGTMSLWQGVDVPGPSCILVAIDRI
                     PFPRPDDPVMSARAAQIDASGGSGFMQVSLPRAALLLAQGTGRLIRSIDDRGVVAILD
                     SRIVNKRYGSVLLNSMPPLWRSSDGAVIKESLKRLSSQYNSTGQ"
     gene            45960..46670
                     /locus_tag="PLW53_01090"
     CDS             45960..46670
                     /locus_tag="PLW53_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36054.1"
                     /translation="MRITLVAMLIIFSSMNATYGACEGETCIDVRANQESNEVVITVK
                     KGKAGSQVSTAPKPRPTSTTKRTWIPWLPKPPAASKPVALGPKPSPKPRVKRISGSQI
                     SDQVKSLIPRGAIITQPLDNILVNQSVNFLTNTPMHFTTVLVVLGTPITIHLTPEFLW
                     EFGDGNSQSTKLAGAPYPLMLIENTYKSSGQKRVELTTTWSGFWRAGSIGAPIDGAIV
                     QRVSRQIDVRPARGIYRG"
     gene            46690..47727
                     /locus_tag="PLW53_01095"
     CDS             46690..47727
                     /locus_tag="PLW53_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HRD36055.1"
                     /translation="MKKTSISLSQDALPMTTLTTPKDLLAAVPFLIGFNPENSIVLLS
                     LKNDAIAMAIRIDFPDQIGADELKLLNSHLSRDSAEAIIAIFYCPEIGQSQNEIISEI
                     LAAITEYGLNLREVITVEKDRYRSLICEDLSCCPSEGNLLPELIDSRIAAEQVAQGRP
                     IPFATLELLIDSISKLDTDHELLELIRSIEPIDYEKDPISFQRQGASSVNQFMDEFKS
                     HGLVKDKALIALLLVRLADLQVRDYALGSVSTESLDLYFTAWRWLLRLAPEGYIAPVA
                     NLFAAVAYERGDGALAQRALDRADSDDPSYAMSKLLRQVFNSGWAPNSFAQMRSELHP
                     KICSELFSGTI"
     gene            47730..48839
                     /gene="ald"
                     /locus_tag="PLW53_01100"
     CDS             47730..48839
                     /gene="ald"
                     /locus_tag="PLW53_01100"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRD36056.1"
                     /translation="MNIGVPKEIKNHEYRVSLTPAGAREFIRQGHKVLVESSAGLGCA
                     ISDDDYRSVGATIIASADQLWAEAELVLKVKEPIAAEYHRMRAGQVLFTYLHLAASKD
                     CTDALMKSGITAIAYETVELNSNLPLLAPMSEVAGRMAPQVGAAALQASAGGRGVLLG
                     GVPGVRPGKVLILGGGTAGLAAATIALGMRADVTVVDLNQSRLAYIDQIFNGAVKTLV
                     SNTHEIEQEALLADLIIGAVLVHGAKAPKLISNELVSHLKKGSVLVDIAIDQGGCFED
                     SKATTHADPTYKVHESIFYCVANMPGALPLTSTYALSNATLPYALALANKGWMKALSE
                     NSGLAKGLNVHNGQITYKAVAQAHGMASVEMDFSL"
     regulatory      48860..48972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            49025..49876
                     /locus_tag="PLW53_01105"
     CDS             49025..49876
                     /locus_tag="PLW53_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD36057.1"
                     /translation="MKKIFKFIPLLLTALVITACSSSSDEGATGDSTTCSPESLATVE
                     AGVLTIATGEPAYYPWVIDDAPESGEGFEAAVAYAVASELGYDKSAVKWVRTSFDAAI
                     APGPKTFDFNLQQYSITDERKQVVDFSSAYYKSNQAIVSFKGSKIDGVTTLQGLADAG
                     AKLGAAVSTTSLDVIEGQLGLKAAVFNDNAAAVTALKNKQIDGLVVDLPTAFYLSAVE
                     VPNGIIVGQIDGSDSGDQGFGLLLSKDNPITSCVSGAVDAINNNGTLQAIIEKWLTSS
                     AGAPVLG"
     gene            49910..50821
                     /locus_tag="PLW53_01110"
     CDS             49910..50821
                     /locus_tag="PLW53_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRD36058.1"
                     /translation="MAKKSGRSSNRAAPSSSYNFGGSWSPSEIELARRAARRARNKRS
                     ALIAALSSFLVLGTLGLVLVTSPGWETLRNTFFKWAYGFEILPKILLGFTTNATLTVI
                     AGTSVALIGLAIALLRTSRSPALTPFRILATVYVDVFRGIPMLLVILLVGFGIPALQI
                     KGLTNNVLVLGTLAVIITYSAYVAEVIRSGILTVHPSQRAAARSLGLSNFQTLRHVVL
                     PQALKRVTPPLLNDLVALIKDTGLVSILGVTDAVRAAQIQTAKTFNYTPYVMAAIIFL
                     LVTIPLTRWTDRMLRNSYERENAVGQA"
     gene            50818..51567
                     /locus_tag="PLW53_01115"
     CDS             50818..51567
                     /locus_tag="PLW53_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRD36059.1"
                     /translation="MSQAVLQLEKVRKSFGSEVVLEDISFKVLEHKATVFIGASGSGK
                     STLLRCINLLDQIDDGQILLDGQDISAADVNPDEVRKKLGMVFQSFNLFPHMTVLENV
                     ILAPTKVQKVAKEKAIDEALELLNKFGLREKADQYPDRLSGGQQQRVAIIRSLARHPR
                     LLLLDEITSALDPVLVNEVLSTVRDLKSEGMTMVLATHEMGFAKQVADELCYLHQGRI
                     LEWGSPKKILENPKKLETKEFLRRLHQAGRL"
     gene            51744..53156
                     /locus_tag="PLW53_01120"
     CDS             51744..53156
                     /locus_tag="PLW53_01120"
                     /inference="COORDINATES: protein motif:HMM:NF004561.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRD36060.1"
                     /translation="MAVSRAPKNAGKKKSVAKKSTKSVAKSSIKKDKKGAKSKGPRLF
                     PTKAELEAMKLAREAKAKPAEKSGPRRFPTKAELEAAKAAAAGVSKPAGKKIIEKVDG
                     EEVELEEVELEDLEKLVAETGEDVVEGASDVRVVNLTEEAKKEEGEFTLKDSEEDDAP
                     PQTVLTAGATADPVKDYLKQIGRVALLNAELEVELAMRIEAGLFAEAKLNSGEKMERS
                     FKRELEWIAEDGKRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEK
                     FDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGR
                     EPTPEELAKELDMTPEKVVEVQKYGREPISLHTPLGEEGDSEFGDLIEDSEAIVPADA
                     VSFTLLQEQLHSVLDTLSEREAGVVAMRFGLTDGQPKTLDEIGKVYGVTRERIRQIES
                     KTMSKLRHPSRSQVLRDYLD"
     gene            53167..53358
                     /locus_tag="PLW53_01125"
     CDS             53167..53358
                     /locus_tag="PLW53_01125"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRD36061.1"
                     /translation="MSEKVKIFVKPNGSIRVTGEVDFVDAQGNVIESKENFSLCRCGH
                     SKEKPFCDGSHRDAGFTAE"
     gene            53321..54664
                     /locus_tag="PLW53_01130"
     CDS             53321..54664
                     /locus_tag="PLW53_01130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD36062.1"
                     /translation="MAPIEMQDLPPNNSLWAEGFKQSGKRKEFNNSYDVAIIGGGFSG
                     LWSAYHLLKLNPKLSIVIFEAKSIGYGASGRNGGWVSSDYPVYRKTLEKRHGSDATNN
                     LFKELANAINEIGQIAGQIAPKASFVKSGTLTFARNKAQERRLKESADSEHFWKSAGE
                     LDQLIQVSDLRGGLFNPQCATINPFQLVLGLAKYLESKGVVIAEGVFATQTNGGVLAN
                     SSLVKAPVVIRASEAFSNPGREFIPLYSLMIASEALPEEIWDQIGVKDRFTFAEGTHL
                     VNYAQRSADNRLAIGGRGASYPFGSKLIEEKEMTSSVHQSLEKMARRWFPVLKNVKFT
                     HAWGGAVAITRNWEPYVQFDKSTGSARLGGYAGDGVTMSYLASKILADLIVGRESNVT
                     NLHFVNREIRKWEIEPLRYLAVNSLVKLSGIADREEARTGRPSLVSRLISPLILR"
     gene            complement(54639..54857)
                     /locus_tag="PLW53_01135"
     CDS             complement(54639..54857)
                     /locus_tag="PLW53_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36063.1"
                     /translation="MTENLLLTSSPLSALDRCDRCGAQAYVRAVMANGFELLFCAHHA
                     KKYQDGLASTAARIDDETERLSAESTAK"
     gene            55047..57134
                     /locus_tag="PLW53_01140"
     CDS             55047..57134
                     /locus_tag="PLW53_01140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRD36064.1"
                     /translation="MAETSSADQVRSVEKSYSAKDLSVLEGLDAVRKRPGMYIGSTDG
                     RGLMHCLWEIIDNAVDESLAGYCKKIEINLEKDGSIEVHDDGRGIPVDKEPKTGLTGV
                     EVVMTRLHAGGKFGGGSYAATGGLHGVGASVVNALASRLDVEVDRDGKIYWMSFKRGV
                     AGIFDGDGPNAPFEPKSGLRIIGKVSAKITGTRIKWWSDKQIFLKDAQHDLEEIYTRA
                     RQTSYLVPGLTLVVNDNRSKDKSSEIFFHKGGISEYCHFIQPDEAVGDVIRLTGQGHY
                     QETVPVLDEKGHLEPQEVERDMGVDIAIKWGNGYESTVRSFVNIIATPKGGTHVQGFE
                     KALVKTFNEFLRAKGVLKKNELDVIKEDILEGLTAVVTVRMQEPQFEGQTKEVLGTPA
                     VTKIVTQVVSDELKKFFTTTKRIDKANGLVVLEKVANASRTRISSRAHKELQRRKNAL
                     ESSSLPAKLSDCRSEDVSRTELFIVEGESALGTTKAARNSEFQAILPLKGKILNVQKA
                     SLSQMLDNAECAAIIQVIGAGSGKSFTLEDARYGRVILMSDADVDGAHIRCLMLTLLY
                     RYMKPLIDDGRVFAAIPPLHRIEVIGGGGKKGEVHYTYSDDDMKKLLTDLKKQGKRWK
                     EPLQRYKGLGEMDADQLRETTMDPDKRTLRRITIKDAAEAEKIFELLMGNEVAPRKEF
                     IATADIDRERIDA"
     gene            complement(57131..58648)
                     /locus_tag="PLW53_01145"
     CDS             complement(57131..58648)
                     /locus_tag="PLW53_01145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HRD36065.1"
                     /translation="MAKSDSGKSTGKKVVAAKPGEFPGKIVDIDVSSEMSDSFLEYAY
                     SVIYSRALPDARDGLKPVHRRILHQMSEMGLRPDKGHVKSARVVGEVMGKLHPHGDGA
                     IYDALVRMAQNFSMRLPLVDGHGNFGSLDAGPAAMRYTEARLASAALMMVEEADEDTV
                     DFGANYDGQILEPLVLPAAFPNLLVNGATGIAVGMATNMAPHNLGEVIQATKHLIENP
                     SATLKQLMKFIPAPDFPTGGSIIGKEGILEAYQSGKGAFKVRAKVEIGKVSARKSGIT
                     ITELPFNIGPEKVVERISELVKSKKIQGISDIIDLTDGQSGTKVVVEVKNGFEPEAVL
                     ENLYKLTPLEDAFSINAVALVNGKPKTLGLKELLQVFIDHRIQVVTRRSNFRKAKATA
                     RLNLVEGLLKAIIDIDKVVKIIRGSDDANEAKESLIKSFKLSEEQATYILDMPLRRLT
                     KMSKIELDTEAKDLKSTISKLTALLKSEEALRNQVSDELSEVSAKYATARRTLIA"
     gene            complement(58677..59567)
                     /locus_tag="PLW53_01150"
     CDS             complement(58677..59567)
                     /locus_tag="PLW53_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRD36066.1"
                     /translation="MAQILCIGDAMLDVIVKMQGQMYLGSDTISQISTHGGGAAANTA
                     TWLASSGHKVFYVCRLGDDAAGRVIANEFDAWNIEYKPEFLKEHRTGIVVVLVDQNGE
                     RSMFPDRGANSGLSELDLPDLTRFSAVFLSGYALFNPQSQAGVLRMINKMREANLEIF
                     FNPGSVGVMSNLGVDACRQRCKLMDLLIMNQAEAEFLTGNSDIEGALNELSKDVETVV
                     ITTSSQGAIGKSRGQEIVNSPILPITAIDSTGAGDAFAAGFIGRWIESKDLESSLRAG
                     NTLASGCVTTIGARPSVNPK"
     gene            complement(59570..60463)
                     /locus_tag="PLW53_01155"
     CDS             complement(59570..60463)
                     /locus_tag="PLW53_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRD36067.1"
                     /translation="MIKYSAAVARALKDKSPVVALESTIITHGLPRPKNLEVALEVEQ
                     IVTETGATPATIAIIDGQIHIGLEPDQLTQIANDENILKASIRDLAIISTQKKSAATT
                     VAATSHIANLAGISLFATGGLGGVHREAWQSWDESADLLALANTPILIVCSGAKSILD
                     VSATLERLETLSVPILGYKTNKFPGFYLTDSGFDLEHRADTPQDIAQIWRARSDVATN
                     NSALIVANPVGNQMDKALHDQILFDGLAKAKTAGIIGKAVTPFLLEYFHSNSKGESLR
                     VNVEIIKANAALAAQIAVALR"
     gene            60462..61595
                     /locus_tag="PLW53_01160"
     CDS             60462..61595
                     /locus_tag="PLW53_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRD36068.1"
                     /translation="MRGLGYWKDGVVAYLLSDLSQSIFATLGVSNCINSLGLSNNEGQ
                     RECLLLVDGMGINALEIVGKELPIFSQIKNHNQLQATFPSTTSASLTSLGTGCAVGVH
                     GMVGYTMRVPNSGTPERLLNALKWDERVDPYIWQSEATLFERAKKQGVKVSHIAAKRY
                     EETGFTRAALRGADYYGVNQVDEMVDQAKSALSNTRSFAYLYLNDVDDASHREGLGSE
                     KFHYAMAKSADLILKLIENLPKGSRLWVTSDHGMINRDDFVVLGKENDLLKNVELLGG
                     EPRVRYLYLKPGSVDETKSQWQEFFKEKVTLYTRQEAIALNLFGPEVLDKNMERIGDL
                     IAIANGEFIMVEGERQELQLSMVGHHGGITQAETAIPLLSADI"
     gene            complement(61600..62403)
                     /gene="sepH"
                     /locus_tag="PLW53_01165"
     CDS             complement(61600..62403)
                     /gene="sepH"
                     /locus_tag="PLW53_01165"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HRD36069.1"
                     /translation="MTDLRFIARSQDGEHLELSDSDGNNFSLRVSDTLRAAVNERRLA
                     TVVESAPQFSIKEIQARLRAGESFAEVARISGLSIEKVERYASPILQERAWIIELAEK
                     ASPKGTSLPLSELVIQRLAPRGVNMNQISWNTWRLDDGSWHLVLTYPSRDGQSEATWS
                     FDANKRTLHSRDDGARWINGEEVSQKSAPKNDRLSDHGVLFPMEGESPQPPRLVAVRT
                     DPNLLEETSGVKEIAPDAKKDGVTKRISIPSWDDIMFGRGKKKDEQEEE"
     gene            complement(62450..63664)
                     /locus_tag="PLW53_01170"
     CDS             complement(62450..63664)
                     /locus_tag="PLW53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36070.1"
                     /translation="MLTSYKRLFAVPGGWSFSFAGFILRMPISMLYIAIVLFVVAETG
                     SYALAGALSMVASLILSVATPLWSRVADQIGQNKVLSITAPVHLIFLGLFVYLVKSDA
                     PTWSWFTAAIIFEGFVIGSGQMVRRRWIHVIGDDRKLIDTAYSFEALVDEVIFTSGPV
                     LAAITASYFFPEAAIFTAMGFISVGTLLFLSQRRTQPPAHPREVGDDHSLLIKDRFIQ
                     ALFIPMMAVGAFFGSTGLVLVGYLDEFGIREYSGFYIAIWSFSSGISAFISGSLHWKI
                     NEARRFILSVFALALLTLPIYFAAQIFTGNLTILAIALFINGLAIAPLLTAGFAVAER
                     SVSPKRTTEVLAWSISALNLGGAIPAAITGYIIDTYGSTVAFMVPVACMLIALLSLLP
                     FLSLWKAKVIQL"
     gene            63712..64488
                     /locus_tag="PLW53_01175"
     CDS             63712..64488
                     /locus_tag="PLW53_01175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD36071.1"
                     /translation="MSLNLELLALAEKIALSAGELLLDRPSKFELDEKSGALDFATQK
                     DHQSESLIVSQILAQRPLDGLIGEEGANKESESGVTWVIDPIDGTVNYLYDLPGWCIS
                     IAAKNREEYIAGVVYSPTTNSLWKASKSNGAFLNNSPIKCNDPVNLDRALVGTGFAYD
                     INNRKVQAKIIEELLPKVRDLRRLGACAVDICHVGSGALDAYFEEGVNEWDYAAASLI
                     AQEAGATFSVTKARDGSSRSSVIVAGPFLHAALSAQIQGQ"
     gene            64620..64958
                     /gene="rplS"
                     /locus_tag="PLW53_01180"
                     /pseudo
     CDS             64620..64958
                     /gene="rplS"
                     /locus_tag="PLW53_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251752.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            65156..65836
                     /gene="lepB"
                     /locus_tag="PLW53_01185"
     CDS             65156..65836
                     /gene="lepB"
                     /locus_tag="PLW53_01185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRD36072.1"
                     /translation="MSPKAKGSSLRELPILIISALVLSIIVKTFFIQFFYIPSGSMEN
                     TLQVNDRVGVNKLGALFSDIKRGEVVVFRDPANWLSPNYDDTSGFRKVIKDSLVFVGV
                     LPDPSKQYLIKRVIGVGGDKVRCCGKDGKIEVNGVSINEPYIYEGDKPSDSEFEVEVP
                     QGFIWVMGDHRGASADSRFHTDDPNKGMVPLDKVTGRATFIIWPFSNLAVLEKGEDLS
                     KIPVKSSK"
     gene            65840..66442
                     /locus_tag="PLW53_01190"
     CDS             65840..66442
                     /locus_tag="PLW53_01190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRD36073.1"
                     /translation="MIESKLIQSGLTKIAGVDEAGRGACAGPLVIAAVILKDITDAKL
                     AKVRDSKELKPGMREELFEVIVDLAQDYSIIEISAAEIDVIGLHKANLEGMRRAINAL
                     EVEPEYVLTDGYEISGLGSESLAIWKGDQVAISISAASILAKVYRDRIMINLDHQYPG
                     YHFADHKGYVTRTHERSLNELGVTAIHRKSYANIQALINK"
     gene            66506..67432
                     /gene="dprA"
                     /locus_tag="PLW53_01195"
     CDS             66506..67432
                     /gene="dprA"
                     /locus_tag="PLW53_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRD36074.1"
                     /translation="MIDMENQTNDDLEIQARLKLFSVIEPGSISWSVELKRYGAVELV
                     ERIIHGPYEKFGRESEGYRQLLRDTSIQKLWEEIEIADARFITPNSKHWPTSLNDLRN
                     PPIGLIIKGANLPAQSISIVGTRKPTLYGARVASEFASGFADRNWAVISGGAYGIDTH
                     AHRGCLAAEGSTFAVLASGVSVNYPAGNNKLFSELQENGALISEVMPSVGARAERFLT
                     RNRLIAALSKATIVIEAAFRSGSLRTARDAAEIMRPVMAVPGPITSPTSDGCHRLIGE
                     RLAEIVTSVPDALELILPISGQKTGTLSGDDT"
     gene            67422..68345
                     /gene="era"
                     /locus_tag="PLW53_01200"
     CDS             67422..68345
                     /gene="era"
                     /locus_tag="PLW53_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRD36075.1"
                     /translation="MTHKAAFVAIVGRPNTGKSTLVNALVGEKIVITSHHPNTTRNAI
                     RGIVTRPDFQLVVVDTPGVHKPKTLLGGALNSIVNENMESVDAIVLCIPAVEAIGAGD
                     LFIAKEMARHRSAKKICAITMIDMAKKALMPEQLIAASKLAEDAGFTWDEVVPLSAKT
                     DDQVQLLMDLLAKYAPESPPFYPADMSSDQELNTQIADLIREAAIEDVFEEVPHSIAV
                     IIDEISERDKRVEGDTPFFDIHASIIVERDSQKAIIIGHKGERLKQVGTVARGEIEKK
                     LKARVFLGLHVKVMANWQSDSKALLKLGFTR"
     gene            complement(68335..69213)
                     /locus_tag="PLW53_01205"
     CDS             complement(68335..69213)
                     /locus_tag="PLW53_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD36076.1"
                     /translation="MSRKGWALFALVGLLWGVPYLFMKVAVEELSTPVIVFARLAIGA
                     LLLIPIALYEKSIRPALKYWPYILFYSVLEMVIPWYLITTAQRDLSSGLVALLVSTVP
                     IWATLFAHHSGDSTAAHRTRIFGIAMGLIGVALLVGIESLNDFDSAIAIAMVLVASIS
                     YAWAVNMITRRAPQISGVAINGLSMLIALIFFAPFAAFNLPTSSPSLEAISATAALGI
                     FSTGLAFWIFFMVLKEIGPARASLVVYPNTAVAVILGIIILNEKLTLAITIGLPLVLV
                     GSYFASKKSETPVVTG"
     gene            complement(69214..70380)
                     /gene="glgA"
                     /locus_tag="PLW53_01210"
     CDS             complement(69214..70380)
                     /gene="glgA"
                     /locus_tag="PLW53_01210"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRD36077.1"
                     /translation="MSGAKVKVAMITREWPPEIYGGAGVHIANLVSALNQSGEIESFV
                     HCFGGARSDATNYQLIKEFSTINPALQTLLLDSDIAKNLKGMDVAHSHTWYANLAGYL
                     AMKIFGIPHVITAHSLEPLRPWKAEQLGGGYQISSWAEKISYESASAIIAVSDGMKAD
                     VLKAYPEINSDKVITIRNGIDTKAFAPNPKLEVLKKYGINSPYALFVGRITRQKGLAH
                     LLRAWKRVPKDYSLVLAAGSPDEPAIGAEVEQLIAELSKQRENIIWIKQMLERDELTA
                     LLTGAQVFLCPSIYEPLGIVNLEAMACETAVVASKVGGIPEVVVDNETGLLVDYSPNS
                     SEFEDALASAINRLMSDKSLSVKYGQAGRIRAIEHFGWDKVAEATIELYRSLIK"
     gene            70404..71627
                     /gene="glgC"
                     /locus_tag="PLW53_01215"
     CDS             70404..71627
                     /gene="glgC"
                     /locus_tag="PLW53_01215"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRD36078.1"
                     /translation="MARLDLPLGIVLAGGEGKRLMPLTADRAKPAVPFGGSYRLVDFV
                     LSNLVNAGFLRIAVLTQYKSHSLDRHITTTWRMSTLLGNYITPVPAQQRLGPRWYTGS
                     ADAILQNFNLIHDENPKHVIVFGADHVYRMDSNAMYLSHLESGAGVTVAAIRVKRSEA
                     SEFGVIELDSDGVTIKAFHEKPKDPPAMPGHPDLCLVSMGNYIFRRDAMEEALILDSE
                     DVDSRHDLGANIIPAMTRAGLAKVYDFANNVVPGETERDKGYWRDVGSIDTYFEAHMD
                     LVSPLPIFNLYNRQWPILTNPPSLPPAKFSERGIAFDSVVGAGSIISGGELRRTVASY
                     DVYAGRDSVVESSVLMPAVRIGDGARVKNAILDKNVIVEPGASIGFDLERDRQRFTVS
                     PGGIVVVGKGVVVTR"
     gene            71713..72738
                     /locus_tag="PLW53_01220"
     CDS             71713..72738
                     /locus_tag="PLW53_01220"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRD36079.1"
                     /translation="MRFGVLTGGGDCPGLNAVIRAVVRKGEKEYGYEFIGFRDGWKGP
                     LEGLTMPLNIAATRGILPRGGTILGSSRTNPFKIENGVERIKENLAKMNVDALIAIGG
                     EDTLGVATKLDALGVKVIGVPKTIDNDLNNTDYTFGFDTAVNIAVEAIDRLHTTAESH
                     HRALIVEVMGRHAGWIALHSGLAGGASCILIPEVPFDIDKVCSYVKSRFEQSYAPILV
                     VAEGALPKDGDLITKDSTKDAFGHVKLSGIGEWLANEIEKRTGAEARTSILGHIQRGG
                     TPTAFDRVLATRFGLHAITAAHEGDWGKMVALHGTAIKRVPLASATEKLKTVDPALLA
                     EAEVFFG"
     gene            72780..74114
                     /locus_tag="PLW53_01225"
     CDS             72780..74114
                     /locus_tag="PLW53_01225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRD36080.1"
                     /translation="MSIDSLFDPALKAAQQPNWPDRSELELAVKELKSLPPLVFAGEC
                     DNLKEKIALAADGKAFMLQGGDCAETFLAATADSIRNRIKTILQMAAVLQYGASLPVI
                     KVGRMAGQFAKPRSNDLETRGEVSLPAYRGDAVNDLEFTPEARRPNPARLVKVYQTSA
                     STLNLVRAFTQGGFADLRLVHEWNKGFVKDSRIGVRYEAMANEIGRALEFMRSAGINP
                     EEFKTVDFYSSHEALILEYEKALTRIDSRTGNAYDVSAHFVWIGERTRQLDGAHIDFA
                     KKIKNPIGVKLGPKTTAASALEIIQALNPDNEPGRLTFITRMGAQNVRSVLPGIIEAV
                     NKSGAKVLWVCDPMHGNTYEAPSGYKTRKFDDVLDEVKGFFEVHQKLGTHPGGIHIEL
                     TGDDVTECVGGGEEISHEDLATRYESACDPRLNHTQSLELAFLVAEMLRDRS"
     gene            74152..74643
                     /locus_tag="PLW53_01230"
     CDS             74152..74643
                     /locus_tag="PLW53_01230"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRD36081.1"
                     /translation="MLLRTTLKTPVGALSLISREHILIAAGFTSQDELLSSISKQDRQ
                     LESKKVSQIPIISDLVSDYFDGDLRALDGIKVDQEGEKFSQSAWRVMRKIAPGRTLTY
                     ADLAKRAGSEDAVRAAGSACARNLIALVVPCHRIVKTGGALGNYAYGLKYKQWLLSHE
                     GAL"
     gene            complement(74617..75681)
                     /locus_tag="PLW53_01235"
     CDS             complement(74617..75681)
                     /locus_tag="PLW53_01235"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRD36082.1"
                     /translation="MADSTEFIDLRSDTVTKPSLQMRSAMASAEVGDDVYSDDPTVNS
                     LEERVADLFGKEAALFTPSGSLANQLAIRSLVSPGQELICETNSHIARAELGAAATFS
                     GITTRTWLADRGILNAAQVLDLAKPDSGPYLVSTTAIAIENTHNFGGGSIQPIEEIAL
                     LSRRAKELGLFLHLDGARIWNAHVASGVSLLEYGKHFETISVCLSKGLGAPVGSLMIS
                     SKERISSSRVWRKRYGAGMRQIGILAAAGHYALDHNISRLVDDHRKAKQLAIACASVA
                     PSTINPDFVETNIVGLDLSGANVSALEFASDAKQKGLLVSALGKSYLRLVTHMDIDDE
                     ANERACAILQELLKRAFMAK"
     gene            complement(75694..76554)
                     /locus_tag="PLW53_01240"
     CDS             complement(75694..76554)
                     /locus_tag="PLW53_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD36083.1"
                     /translation="MQLKLAPWALLAVAAMWGISFVWMKDILDQQDVYSFLTSRFIFA
                     ALAMIIIKPKVLKLFTKELVSKGFIIGLALGSGYIFQTLGLDRTTPAITGFITGLYVV
                     ITPLIAALLLKERVTLTMWGFILLAVVGLAVLSVHGWSVGFGEFLVFISAILFAVHII
                     MLGAWSKNFDAYALTVMQLIGCAFLSAIPAGINGFNPPPDTQVWAVVIFTAVFATAIA
                     FVIQTWSQARISTTKVAVILTMEVVFAALFSFAYGMEPFTLRLAIGGTLVLIAMLAIV
                     QPRFSSPRAS"
     gene            complement(76554..77417)
                     /locus_tag="PLW53_01245"
     CDS             complement(76554..77417)
                     /locus_tag="PLW53_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRD36084.1"
                     /translation="MPKAAKPRIGAHVPTSGGMAKRAIEYAKTIKAEAIQVFASNPRG
                     WAMPETNSDADQAFIEKASELDIETYIHAPFLINLGSPTEGTYKNSLASTKYSLKRGR
                     EIKARGVVIHTGSAVDEGNIKKAWKQIHEGMMPILSELDDQDPWLLLEPTAGQGQSLV
                     KRLEDLNNYFEALEWHPKVGVCLDTCHVFAAGHDIKSKGGMSATIDLLVEVAGSERIQ
                     LIHANDSMDVLGSLKDRHQNLGDGEIGTKPFAELLAHPAIVNAPLILETPGEEPEHGR
                     EVAMLMKMRDK"
     gene            complement(77422..79332)
                     /gene="pknB"
                     /locus_tag="PLW53_01250"
     CDS             complement(77422..79332)
                     /gene="pknB"
                     /locus_tag="PLW53_01250"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRD36085.1"
                     /translation="MSELIGEIIDGRYQLTRVVGSGGMATIYAAIDLRLDRQVAVKIM
                     HSHLAQDEQFVSRFIREAKAAASLSHPNIVAVLDQGWNQGGSPCVFIVMELIEGATLR
                     DYLIEQGSLSPERALSIITPVASALAAAHKLGIVHRDIKPENILVSKEGRIKIADFGL
                     ARGALLGNTMTAESSVILGSVSYLSPEQVQRGVADARSDIYSLGIVLFEILTGQKPYQ
                     GEDPVQVAIKHVNERVPAPSTLKPGLSVEIDQLVLSATDIDPDKRPRDAVVMLEKLRE
                     LSEKLDPRKRQLSLELDLPPLAIKEVGKRDGAKRLRRDKDREVEIPKNQEAAVKKEKS
                     SSITKKALSKRVKRNRQIAALIAIALGIGIWYVIVGPGSKVIVPSLAGLTVKEATSEL
                     ADLGLDLKVEREEFSEDIPENRVIKSSPAGGGRISPDGTVEVTISKGKERYIVPTLQG
                     LKIEIAEGLISDNNLVVGEIIEEFSSEFPKGFIMRSSPAAGERIKRDSQVTLYLSKGV
                     EQVGVSSYQGKSGEQALNELTEAGFDVETKYVFSEDLPIGAVVSQIPSGGDIDKGSVV
                     TLTVSKGSQFVFIPNVFSLSEAKAIDTLKDLDLKVVVKKVGNKKTKVVTNISPKVGDK
                     VKRGSTVTITVG"
     gene            complement(79342..79929)
                     /locus_tag="PLW53_01255"
     CDS             complement(79342..79929)
                     /locus_tag="PLW53_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRD36086.1"
                     /translation="MLIKSNMNQPSLTEIITSIKKGRAIGNVGRIVAIDGPAGAGKTT
                     LANRLKENIQESSVEIVHMDDLYDGWQNSLTPALGKVLDISICSPVSKGKSFQYRKYN
                     WIESKFGPLLDFTFPEILILEGVGSGQRVVRKYLDELIWIDISAQVGLQRVLQRDGES
                     IEKEMEIWQMRENYHFVADNTRDSATIRIDGATFI"
     gene            complement(79913..80497)
                     /gene="pnuC"
                     /locus_tag="PLW53_01260"
     CDS             complement(79913..80497)
                     /gene="pnuC"
                     /locus_tag="PLW53_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRD36087.1"
                     /translation="MFTIFTAWGYEVSALELSAVITSFTAVLLGARGVRMTWPWYLVS
                     ASLYAIFFYQVDLIASALLQFVFIAAGIWGWLGWNRSGVIPRYMNNKERLIWLTALVA
                     SWLLTAPILESIGAAATLPDSFLLISSTLAQTAMVLQRNETWIAWIVIDIFGTYHYAN
                     QGYWFTALLYGVFTIIAVFGLERWKKLSKSADQI"
     gene            80609..80941
                     /locus_tag="PLW53_01265"
     CDS             80609..80941
                     /locus_tag="PLW53_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRD36088.1"
                     /translation="MLKFGYFAMLTFTVLGSFWLEIFLKVGVLRRIKRAMLAILPTAA
                     IFLTWDYYAVTSRHWFFDREQVVGIFGPAGIPLEEFLFFIVVPLAALMTIEAVRRVKP
                     HWNYGDER"
     gene            80938..81243
                     /locus_tag="PLW53_01270"
     CDS             80938..81243
                     /locus_tag="PLW53_01270"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRD36089.1"
                     /translation="MSYTQLALLSVPLVILFDFYITKSRVLSRKSFWNAYAIMLFFQI
                     ITNWWLTSRGIITYDPDVIVGVRIASAPVEDLFFGFSMIVMVISQWIFWGRRGIQRN"
     gene            complement(81240..82148)
                     /locus_tag="PLW53_01275"
     CDS             complement(81240..82148)
                     /locus_tag="PLW53_01275"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRD36090.1"
                     /translation="MDELSAAGIHDPELRKSYLECKRLNSLHGKTYYLATLLLPKAKR
                     PHVHALYGFARYADEIVDDLASTLTHQEKVDELGRWSDQLLSDISMGRSSDHVGRALV
                     DTVQRFNIPISYFEAFLHSMAMDLSVTQYERYEDLMEYVYGSAAVIGLQMVHVLGTVE
                     GKKSEALAPAEKLGIAFQLANFIRDVGEDLERGRIYLPMAELASFGVDREMLEARQVR
                     PEIIQALKFQIARVRQLQSEADAGIELLSPDARACIRAASELYCGIVDEVEKIDYQIF
                     TKRAKTSNFRRVKTALPAYFSALASR"
     gene            complement(82151..83632)
                     /gene="crtI"
                     /locus_tag="PLW53_01280"
     CDS             complement(82151..83632)
                     /gene="crtI"
                     /locus_tag="PLW53_01280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRD36091.1"
                     /translation="MRKVKGKTDRVVVVGAGLAGLSCALRLAGAGREVIVIEREAVPG
                     GRNGLMKKDGYSFDTGPSVLTMPDLIADALACVGEKIEDWLELMPLKPLYRAFYADGS
                     QLDVHANTEQMEVEIAKNISADEAAGYRRYVEFVTKLYKYEMNDFIDRNIDSPLNLLT
                     PNLARLIALGGFRKLAPKVSQFLKDPRTQRVYSFQAMYAGVSPQQALAIYAVIAYMDS
                     VNGVFFPKGGMHAVPRALAGAAEKHGVTFKYNTTVTKVEKSGSRASAVITSDGQRIEC
                     DVVVLNPDLPVAWRDLLGKTPLSVKRLNYSPSCVTILAGSSKSYDHLAHHNIHFGHSW
                     DGVFDELITKKQLMSDPSLLVTLPSKDDPSLAPSGKHSYYILFPTPNLDADIDWKKMK
                     NVYKDKMYATMQERGYKDFADSVEVEHVTTPLDWQEMGMERGAPFASAHTFLQTGPFR
                     PSNMAKGYENVVFTGSGTQPGVGVPMVLISGRLAAERIVGPVK"
     gene            complement(83633..84694)
                     /locus_tag="PLW53_01285"
     CDS             complement(83633..84694)
                     /locus_tag="PLW53_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD36092.1"
                     /translation="MKSPDLSQLRAEIDKTLASFTVARSAALVDIGPELVPVAKAMSD
                     FITQGGKRFRPIFAYLGYLGSGAEAREEILRACTSLELVHVCALIHDDVMDGSDTRRN
                     KPAIHKAFQQYHQDSSYKGSSERFGSSSAILMGDLALVWADRLLVESGISGDEFLQVQ
                     SVFADMRDELMAGQYLDVLEGALATTSIDRSLKVARYKSGKYSIERPLHFGAALSGKK
                     ELMSAYSSFGLPLGEAFQLRDDLLGVFGDPEVTGKPAGDDLREGKRTVLIALTMQAVG
                     KQERELLTASLGDEGLDKTQILKLQEIISSSGAVAQVEKLITELRDRAISALRDSQVE
                     TSILSVLEEMATIATQRSL"
     gene            84719..85567
                     /gene="metF"
                     /locus_tag="PLW53_01290"
     CDS             84719..85567
                     /gene="metF"
                     /locus_tag="PLW53_01290"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRD36093.1"
                     /translation="MNSAPTFSVEFFPPKDQAGEERLWSALKALAVYKPDFVSVTYGA
                     GGSTRDRTVRITSEITARSGISTIAHLTCVGSSKDELIEILNQYKASGIKSILALRGD
                     PKGGPGVKWESTAGGFDHADQLVTLASSMGFEVGVAAFPDGHPASMGNFEQDIKVLLE
                     KERCGASFATTQFFFDVKGYINLVDEIRARGSKLDIYPGILPITNFAQLKKMSELAGS
                     PIPSEVQRRVEAAGDTPVDVVKVGIDIASELSKKLLDYGVPGLHFYTMNSAAPTIEII
                     KRIGLR"
     gene            85564..86193
                     /locus_tag="PLW53_01295"
     CDS             85564..86193
                     /locus_tag="PLW53_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD36094.1"
                     /translation="MKKIEFFNSWSSLHGGAEIKGIVRAWLEISFLLCKPLAVLRVTP
                     NSLSIFGLLLGINLVVNIESNWAIALLVFSLIADGIDGSLAILTGKVSRWGALLDGFI
                     DRIVEALWAFSLYLLGAPYQLVLIAWLAAFLQEYMRSRAASLGVTQLGVVTIGERPIR
                     ASILFIALVARAVDMDLATLLSQIWAVLQVISLFTVLNFLRPLLQQSQR"
     gene            complement(86144..87661)
                     /gene="crtI"
                     /locus_tag="PLW53_01300"
     CDS             complement(86144..87661)
                     /gene="crtI"
                     /locus_tag="PLW53_01300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRD36095.1"
                     /translation="MSHHRTSKVSKSVIVIGAGMGGMAAAARLAKRGHKVKVFESASF
                     AGGKCRTEDISGFSFDTGPSLLTLPAVYRDLFVKTGKRLENLVQLNPVDPSFTYIFDD
                     GKRVTFPNLSHSGTVLAITESFGTQAGDAWHQLLTRAEKMWGASRQAFVESELRSPLQ
                     MLKSPSLLRDLKTIAPFTSLRDLVRSYTDNVYIGKIIDRYATYTGSDPRKVPAVLLTI
                     AFVEEAFGAWHVSGGVGTLAKSLESRLSDLGVELHLNTKVDRIITKSGVAIGVRLANG
                     QVHNSDVVIANADADLVYNQLLEKSSRTRSARNSLNKATASLSGFSLLLGLRGSSELG
                     HHTVFFPKDYDAEFDSIFEKRRPVEDPAIYICNPNDSKMKRAGVDESWSVLINAPRHQ
                     PRDGFNWDEKSSKQYSQHIINLMEAKGLDVRSRIEVLEYRSPADLERNVNAPGGSIYG
                     TSSNGARSAFWRARNRSKIKNLFLVGGSTHPGGGLPLVGISAEIVAEAVEENLEQ"
     gene            complement(87658..88746)
                     /locus_tag="PLW53_01305"
     CDS             complement(87658..88746)
                     /locus_tag="PLW53_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD36096.1"
                     /translation="MTFLLDVSAFLIAIMLLLAVFNFFTLRVVSSKNSASSDQSVSIL
                     IPMRNESENVIELITLLKEQANLSNFEIIALDDSSSDNTKELLLGMSQNNLKVIAGRG
                     LEEGWLGKNYACYQLATAATGDYLVFLDADVRLDPRAVASTIESMRAWNWDFISAYPR
                     QIALTFLERLTQPLLQWSWFTTLPLRITQKWPRPSTVVANGQFMVIKRKAYFDCDGHR
                     GVKAEVLDDLFLARNLVRTGAKGGVADGSRVSYCRMYSSANQLIEGYTKSQWSAFVNP
                     LGALIAIALLTLTSIFPFIAGLAGELSGWYLYFAIVMTRVLSGIKTRTIPSTSLLHPL
                     SALIWIYLISFSWIKKYRGELSWRGRKL"
     gene            complement(88753..89559)
                     /locus_tag="PLW53_01310"
     CDS             complement(88753..89559)
                     /locus_tag="PLW53_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRD36097.1"
                     /translation="MSIRNYRPKPDSRRTITPWRNFTLVAVLTVALALQLSYPLIEGE
                     RLRLVTIATVYWAAGAMLLHALFAYGLTYAIRFLLITFTYSLLVEQIGMRTTWPFGSY
                     EYSPSLGYQIFGVPLVVPFAWIMMAHSVFIAARRVAPNLVFIVGGYGLMAWDLFLDPQ
                     MVSAGRWSWEVSGRSVPFQPEIPLSNTFGWLLTGMGLMALLNIFLPKERRSLGSSRAV
                     PEFFLAWSWIGGMVINIFHFDRPGVAFLGGSALGALVIWYFISVKYGRRD"
     gene            complement(89574..90233)
                     /locus_tag="PLW53_01315"
     CDS             complement(89574..90233)
                     /locus_tag="PLW53_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36098.1"
                     /translation="MILVFFWKIKARYIPWAILHMGLDRLALARRSDVKFWKLLGAGK
                     GETFTPRDADVLRWGLLVVVDQDSEFANSKIIKRWNKKSHSQFSGQLSPISINGAWSK
                     KSPFDQIPKASSDWSGKIVAITRARIKWRKNFIFWSSVPPVTSSLHSSPGLISAIGIG
                     EAPIGLQGTFSIWESGEAVKNFAYSGAAHREAIKATHRHAWYAEEMFARFALIKSNGS
                     L"
     gene            complement(90230..91024)
                     /locus_tag="PLW53_01320"
     CDS             complement(90230..91024)
                     /locus_tag="PLW53_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD36099.1"
                     /translation="MISPLYIEKSGAGDPLVLIHGMGSASNAFKPIRSTLDKDFLVVT
                     LDLPGHGKSPYQKGELMDPHSLALKVLEQLSAASIDKFHLAGNSLGGWIAMEMAAIAP
                     ERVRSLVGIAPAGLWLNPYNARYPGTALARFLARYTIKLAPTALHFEIARRLGFADVS
                     PRWQEFSYELCLDAAIAMASAEGYYPAWDGMLLKRFDRQISDSIPVTIIFGDSDRTLP
                     ETSCQERSLAPKHARWIIFSDTGHAPMWDSPLDVIDEIKKAAGAAK"
     gene            complement(91040..91528)
                     /locus_tag="PLW53_01325"
     CDS             complement(91040..91528)
                     /locus_tag="PLW53_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRD36100.1"
                     /translation="MDSILEKAAVKRAQAAFDKHGISGKIKILSDSARTAAEAAAGLG
                     IEVGQIASSLVFKLPDDRSLLVITSGRHRVDTQMVADLLQVPALDRADADYVKEISGY
                     SVGGVAPLGWINEPSITLIDEALNDYQVIWAAAGHPHAVFPTTYQELLQASGAKTARV
                     GN"
     gene            complement(91538..91936)
                     /locus_tag="PLW53_01330"
     CDS             complement(91538..91936)
                     /locus_tag="PLW53_01330"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRD36101.1"
                     /translation="MSIKRASVLFVCVHNAGRSQMASGYLQHLAGDWVEIRSAGSAPA
                     DSINPAVVLAMKEEGIDLSAQRPKILTDEAVEASDVVITMGCGDVCPIYPGKRYLDWK
                     LDDPAGQGIDAIRPIRDQIKSLVKELISTL"
     gene            complement(91933..92577)
                     /locus_tag="PLW53_01335"
     CDS             complement(91933..92577)
                     /locus_tag="PLW53_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRD36102.1"
                     /translation="MIGSKALAEFFGTATLLLAVVGSSFMAERLTQDSALALLINATV
                     TAAVLALIIRSAVNLSGAHFNPVVTLTALLLGKIKALEALVYLIAQVLGAIFGVILAN
                     LMFAEVVFSSSSIQRDGAAQLLGEVIATTGLVFIAFSARKKHVWKLIPLWIFGAYFFT
                     VSTSFANPAVTLARFFTDAPAGIASSSLPGFIAAQLIGTLLVLALLRNRERISR"
     gene            complement(92574..93323)
                     /locus_tag="PLW53_01340"
     CDS             complement(92574..93323)
                     /locus_tag="PLW53_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD36103.1"
                     /translation="MEIALAILTGIAIGAILGYVGAGGSMLAVPAFIYIFGFNPVQAT
                     TAALIVVFIGAASGALPKLRKNEILIKEALVIWAIGLSTNFTGAYFLPQIPERTILTG
                     FALVLVVAGTSMLIPAPKESSERKVSPLALIAISLVIGAITGLFGIGGGFLAIPILIL
                     FYNVAPAKAAGTSLFIIAINTLTGFFAHYRHWDEVNWSIPLVVALMALVISRLASRHS
                     SNISPATLKKGFAFFVFAIATFTLIDTWLIS"
     gene            complement(93412..93825)
                     /locus_tag="PLW53_01345"
     CDS             complement(93412..93825)
                     /locus_tag="PLW53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD36104.1"
                     /translation="MDIVLIVGRVLFSLLFISSGVAHLTKLEAMTGYAQYKKVPAAKL
                     SVIVTGLMLIVGGLYVALGIYADLGALLLAIFLLLSAFKMHDFWKVTDAQAKQSEMTS
                     FMKNLALAGASLIIFVLVGSGGEFGPTITEGIFNL"
     gene            complement(93963..95972)
                     /locus_tag="PLW53_01350"
     CDS             complement(93963..95972)
                     /locus_tag="PLW53_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD36105.1"
                     /translation="MITHTQNPKTQGPSLRLRLSAIASAVALATSALVVLTSPQVTAN
                     TFSVPAAPVIKKAYLASNGIRVVFEKVSASPAVTGYVLSSGAGSCAISLPATTSGVVT
                     LPIITGQSSATVSIKAVNAYGFSPAGTWSKTFTAAELASVTSTSWQTVQLLQLSDLHG
                     AIEGTATNAGTALLTTAFAAERAKNPATFTLSAGDNIGAAPPISSQFEELPTIESMNL
                     MKFDVSGFGNHEHDRNIAHVQKIIGASDFQWVASNYNTLEPLKSGNKAAKSYLVLERG
                     GAKVGFVAGNTEDTKEQIFPGNLSFGGKELVISPDVTGINAAVKAAKAEGADFVVAIL
                     HQGWQENRDGEAKGRLIDLAKQIKGAAVVFGGHSHQTYSSALAGNAAVNGTVIAMTRN
                     AGQEYNRVTLCLDSSRRVRGTTVELVTRANIATLTPNADGAALVKKYKDQLNAKLDVK
                     IGKVNGIFPQGGSPQVQRAGEAAIGNFTADAMRKKYGTDFAYINGGGIRDKFPAATYV
                     AADKSLNRPGTDKVAPYDVTLGDALAVYPFGNTVGVSVITGENLWKALENGVSNWPGD
                     GRFPQISGFKFSFDTTKAVGSRVQSVTKADGTAIAKDSKEYTVAVPDFMIYGGDGYVG
                     FFTPSKAKLRGLLVDVFIEAVTEASSGGKAITIPALDGRITKVNP"
BASE COUNT        27283 a        20260 c        22452 g        26139 t
ORIGIN      
        1 ttttaccttt cttggaattc ctgcctgtga tctagataag cctgagagtt ataagggcgc
       61 tgatgtaatc atcgttggag ctccgataga tagtggaacc tcccatagat ctggcgcaaa
      121 gtttggtcca caagcaattc gtatgggcga ttacctgcct catgatggag agcgaccaca
      181 tcttgcttta agaattgatg ggctaaaaga gcttaaaata tttgatgccg gagatctcct
      241 aatgcctcca ggaaatcttg aagaggcgct attagttcta aagaaagcaa cagagaagat
      301 ctcaagggct ggagcaattc cagtagttct tgggggagat cactcaattg catcagcaga
      361 tgttgcagga attgcagagc atttaggcaa aggcaaagtt tcaatgatcc actttgatgc
      421 ccatgctgat accggagatc ttcaattcgg agccttagtt ggccatggaa ctccaatgag
      481 aagacttatt gattctggat atgttcgcgg cgatagattc ttacaacttg gccttcgtgg
      541 atattggcct gatgatgtaa ctctaaattg gatgaaagat aagggaatgc gctcctatga
      601 gatgacagag attcatcatc gcgggctaac taaagttctt gatgaatcat ttgcaactct
      661 tacaaaagat tgtgatggcg tatttttctc cgttgatatt gatgttgttg atccaggaat
      721 ggcgccagga actggaacac cagagccagg cggaatgact tcaagagagc tactagaggc
      781 ggtaagaaga atctgtctag aacttccagt agttggcatt gatgttgttg aagtatcacc
      841 gccatttgat tcagccgata tcacagcaat tcttgcaaac cgagtagttc ttgaggcagt
      901 tagcgctatt gcaaagcgca aaagaggcga tagttacttg ccaactcaga atttacttga
      961 tcgttaagcc tgagcaagac ctgcttcaag aacttccagc gcctctttaa gaaggtgctc
     1021 tggcataaca agtggtggca agagcctaat tacattgcta taagtgccag cagagagaat
     1081 tagaacgcca ttttgctggc agtacttaat aattgatgtc agagcgcttg gatttggatc
     1141 tttagatcca gggattacta gttcaatagc ctgcattgct ccgcgccctc taacatctcc
     1201 aataattgga tacttcttct tcatagcctc aaggctattt cgcataatta atccaagctc
     1261 atttgctcgc ttgactaagt tttcttcctc aattgttgca atagcgccaa gggctgctgc
     1321 gcaggcgata ggtgatccac caaatgttcc accaagaccg ctggcatgga tagagtccat
     1381 tacatcagct cttcctgtaa ctgctgcaag tggaaggcca cctgcaattc ctttagctgt
     1441 agtgattaga tctggaacga ttgattcatc ctcgcaggca aacatctgac cagttcttgc
     1501 aaagccagtc tgcacctcat ctgcaataaa gagaattcca ttttctttgc agaacttact
     1561 aaggcctgga agaaatccct tgcaaggaac gataaagccg ccttcgccaa ggattggttc
     1621 gataacgatg caggcaacat tttttgcacc aatttctttc tcaatcttat gaagcacagt
     1681 atctaaaact gattcttggc aggacccaga acaggaatcg cagcgatatg gataagacat
     1741 tggcatgcga taaacctcag gagtaaatgg tccaaaacca tctttatatg gcatgttctt
     1801 agctgtcatt gccattgtta gattagttct gccgtgatag ccatgttcaa agacaacaac
     1861 tgcttggcgt ttggtgaaat tgcgagcaat cttgaccgcg ttttcaaccg cttcagcacc
     1921 tgaattaaag agagctgatt tcttcttatg atctcctgga gtcaaacgat tgagcgcttc
     1981 acagacctgg atatatccca tatatggggc gatcatgaag caggtgtggg taaatgcctc
     2041 tacctgctcc ttcacatttt taacaactcg atcagctgag tttccaacat ttacaactgc
     2101 aattcctgct cccatatcga taatggagtt tccatcaaca tcaacaatta ccccgccgcc
     2161 agctttttca actacaacag gaattgccat gccaagtgag gccgagactg cctctgctct
     2221 gcgcttaatt agttcttggg attttggtcc tggaattggg gtgattagtt ttctaacctg
     2281 ctcgagatgt ggccctgaac tcatctgctt attctatctt ttccatctat ttaacctatt
     2341 tctgagaaga tggacccgtg cgcgatattg tgattttagg ctccactggc tctatagggg
     2401 ttcaagcctt agaggttatc gctgcaaacc ctgaactttt tagagtagtt ggccttagcg
     2461 cagggggaaa caacttaacg ttattggcca agcaggttag agattttaac gtccctatag
     2521 ttggagttag tagcggagcg caagaattaa agagcgcact atctggagtc aaagttatag
     2581 atggaaccca agcctcagag cagattgcat ctcttacttg cgatttagtt ctaaatgcaa
     2641 tcaccggatc tattggcctt ggtccaactc ttgcagctct agcttcagga aataaagtag
     2701 cccttgccaa taaagaatct ctagttgctg gtggagattt agtgatggca tatggaagcg
     2761 agaagataat tccagttgat tccgagcact ctgctctctt tcaagcgatg agcgcaggac
     2821 gcgcaagtga tgtttctaaa ttaattctta ccgcaagtgg cggccctttt agagatcgaa
     2881 aagatttatc taaagtgaaa ttagcagatg cacttgccca tcccacctgg tctatggggc
     2941 cagttgttac tatcaactct gcaacgctag taaataaagg tcttgagatt attgaggcgc
     3001 actatctctt tgatattgcc tactcaaaga tcgaagcagt gattcaccca caatcaatcg
     3061 ttcactcact tgtggaattt aaagatggct caactcttgc tcaagcatca cctcctgata
     3121 tgaaggggcc gattgcatat gcgcttgctt atccaaatag ggtcgctggg gctatggcgc
     3181 caattaattg gagcgccaag caaagttggg attttgaacc aatagatgag aagagatttc
     3241 cggcagtgca acttgcaaga agagctggcg ctctaggatc ttctgttgct gcaatttata
     3301 acgcagcaaa tgaggtggca gttgctgcat ttatgaagga tgagatcgct ttcacagcaa
     3361 tagtggatac tattgaaaag gttgtggata agttatcagt tagtgcacct tctagaatta
     3421 gagatatctc agatgtcagc gctattgaga aggatgcgcg gatgtatgca aaagaagtat
     3481 tgaagaaagg ctaggagaga aggggttatg ggatttcttg gagtaattgc ctttattctg
     3541 gctctgctta tttcagtgat gatccatgaa tatggtcatt acataactgc taaacgttat
     3601 ggaatgagag tcactgaatt ctttgtaggt tttggaaagc gcctatggtc aacccagcgc
     3661 ggtgagacag agtttggaat aaaggcgata cctgctggtg gttattgcaa gatctctgga
     3721 atgtcgccaa atgaggagtt agctgatgaa tttaaaccaa gagcttttta tctagcatct
     3781 gctcctaaga agttagtagt tcttggagca ggatcttttc tacattttgt tctagcgctc
     3841 tttctcctct ttactctctt tgcaggaatt ggcacatcac aagttcttcc aacgatttct
     3901 caagtacttc cttgcgttcc taaagagagt acctgtaagg ccggtgatcc aatttctcca
     3961 gctaagcgct caggtttaat gccaggagat gaaatcctcg gagtaaatgg tcaggagctt
     4021 gcctggaaag aatcagcaga gatacttcga gcctctgcag agcgagaaat aacattatta
     4081 attaaaagag atggacaagt agtaaatatt gccatcactc cagcaaccag agttgttgaa
     4141 ggagattcca ggggttttct tggaattatt aatgaatttg gtttagtaag acaaaatccg
     4201 ataaaggctg ctgcttcaag tgtaagagca actggagcaa tctttgaagg ctcaattaag
     4261 gctctgattt cacttccttc gcagattcct tctttaattg gccagacatt ttttgggcag
     4321 gagagaaatt cgcaaggttt agttggaatc gttggagttg caagagcaag cggcgagact
     4381 gtttctagtg gaaatttaag tactggcgag aaagttgcaa ccttcattct tattgtggct
     4441 tcacttaata tctttgttgg aatctttaat ctgctgccaa ttcttccgct cgatggtggc
     4501 catatggcag ttgctagcta tgaggcgatc aggcgaagaa ttgcagctat gagaggaaag
     4561 gccgatcctg ggccggttga tgtggaaaag ctcaccccga ttaccttggt ggtctttgcc
     4621 gttcttctag ttctgactgt gattttgctt attgctgata ttttcaatcc aattaacctc
     4681 aatctttagg agagaatagg gccatggttg atctgggaat tccatctgcg ccgccgccag
     4741 ttcttgcccc gcgccgtaaa tctcgccagc taaaagtagg aagcgtttta gttgggggag
     4801 atgcgccagt ttcagttcaa tcgatgtgca ccacacttac cgctgatgtg aactcaactt
     4861 tgcaacagat cgctgagtta actgcttcag gatgtcagat agttagagtt gctgttccga
     4921 gccaagatga tgctgatgcg ctaaaagata ttgccagaaa atcccaaatc cctgttatcg
     4981 cagatattca ctttcaacca aaatatatct ttgcagcaat tgatgcagga tgcgccgcgg
     5041 ttagagtaaa tcctggaaat attaaacaat ttgatgacaa ggtaaaggaa gttgctaaag
     5101 ctgcagggga tgctggaatt ccaattcgca ttggagttaa tgctggctca cttgatccaa
     5161 gacttttatc aaagtatgga aaagcaaccc ctgaggcgct agttgaatca gcgctctggg
     5221 aagctggatt atttgaagag catggtttta gagatatcaa aatctcggtt aagcatcatg
     5281 atccagtaac gatgattaag gcataccgcc ttcttgctgc aaagtgtgat tatccacttc
     5341 acttaggagt aactgaggct ggtccgctct tccaaggaac cataaagtct gcaacggcat
     5401 ttgcggtatt acttgcagag ggaatcggcg acacaattcg agtttcactc tctgcccctc
     5461 cagttgagga agttaaagtt ggtatttcta ttttagaatc tcttaattta agacagcgaa
     5521 aattagaaat tgtctcttgc ccatcttgtg gccgcgctca agtagatgtc tattcacttg
     5581 cagagaaagt acaagcaggc cttgaaggaa tgacagttcc acttcgcgtt gcagtgatgg
     5641 gctgcgttgt taatggacca ggggaggcgc gagaggcaga tcttggcgtt gcatcaggta
     5701 atggcaaagg tcagatattt gttaaaggtg aagtaataaa aacagttcct gaggcgcaga
     5761 ttgttgaaac tttgatagaa gaggcaatgc gccttgcaga ggagatggaa gctgctggtg
     5821 tcgcatcggg agaaccaaca gtaaacgtaa agtaattcgc taggcttagc gctatgttaa
     5881 agatgtcatc tctctttctg cgcacgctta gagatgaccc atcagatgct tcagttgcaa
     5941 gccataagct cctagttaga gcgggatata tccgcccaat tgctgcagga atattttctt
     6001 ggttgccact aggggtaatt gctcttagaa atgttgaaag agttataaga gatgagatgg
     6061 ataaggcggg ctttcaagaa gttcactttc cagcgcttct tcctaaagat ccttatgaga
     6121 aaagtaatcg ctgggaagag tatggacctg ccttatttag gctccaggat cgaaaaggta
     6181 gcgactatct actagggcca acccatgaag agatgtttac tctgatggtg aagagcgaat
     6241 actcctcata taaagatctt cctctttcaa tctatcaaat ccaaaccaaa tatcgcgatg
     6301 aagcccgtcc tagaagtgga attattcgcg ggcgcgaatt tattatgaaa gattcttatt
     6361 catttgattt agatgatgct ggccttgaag tttcatatca gaagcatcgg gctgcctaca
     6421 ttgaaacctt taatcgccta ggacttaaat ttaatatcgt ctctgctgtc tctggcgcta
     6481 tgggcgggtc aaaatctgaa gagttcttag ctccatgctc aacaggtgag gacacttatg
     6541 tgctctgccc taaatgtggg tatgcagcaa atgttgaagc gatggttagt aaggtgagta
     6601 aaaaagagat agcaaaagca cctgagatgg agcttcttga tactccagat actccaacta
     6661 ttgaaagcct tgttgcggtt atgaatcaga agtatcaggc aaatataagc gcatcggata
     6721 ctttaaagaa catcttactt gtggcagatg ggcaggtaat ttcaatacta attccaggtg
     6781 atcgccaggt tgacctcaag agagttgaag caaatcttgc tggagttaaa gagcttcgct
     6841 tgtttgagga tgcagacttc gcaaaaaatc ctgctctggt aaaaggatat gttggtccgc
     6901 aagatgctaa gaaattagga attaagctct atgccgatcc atcagttgta gttggctcac
     6961 actggataac aggtgctaat accaagaata aacatatgcg ctatgtcacc catggtcgcg
     7021 actttaacgt tgatggattt attgaagcag ttgaaattaa ccaaggcgat ccatgtccaa
     7081 gctgtgatag cccaataatt attgatcgcg caattgaaat tggtcatatt ttccagttgg
     7141 gcagaaagta tgcccaagca cttgatttaa cagttcttga tagtcaagga aaatcacagg
     7201 ttgtcactat gggctcatat ggcattggtg ttactagagc tctagctgca attgctgagc
     7261 agagccatga tgaaattggc attatctggc ctgatgaaat tgcgccagca aaagttcata
     7321 ttgtggcaac tggcagggat gatgagccat ttattcaagc ggagagaatt ggaaaagagt
     7381 tagaggcaaa aggtattagc gtgatgattg atgatagacg tgaggcaagc cctggagtta
     7441 aattcaaaga tgcagagcta attggcaatc cttatatctt gatatttggt aagagtctgg
     7501 cccaaggtca agttgaattc cgtattagac gaagtggtga gaagtcagag attgcacttg
     7561 gtgagatcac atcttactta gttaaattgc taggttgatt aactagtttc tcgtggatat
     7621 ctcagttgaa gtaattctat ttctcatcct tgcagccacc tttgcaggac ttgttgattc
     7681 actcgctgga ggcggggggc taattcaact gccagctctt ctagtttcac ttcctgatac
     7741 ctcgccttcg gtgattcttg gaacaaataa agtcccctct tttcttggca ccttaggagc
     7801 tactgcctca tatttaagaa agataaagcc agattttaag ttagcagcgg tgatgggtat
     7861 tcctgcattt attggctcag ctatgggcgc aagtgtggca actaagattc caagagatgc
     7921 ttttaagcca ataattttat ttcttctaat tgttgttgca atctatacct atctaagaaa
     7981 agacttagga caggtttctt ctgcaaaatt tgctggaaga aatcgactat taatcggcgc
     8041 agctgttggg gctgtaatcg ggttttatga tggaatcttt ggtcctggga ccggatcatt
     8101 tctaatgctt gcactagttg cagttcttgg ctttgcattt cttgaagcat cagttacagc
     8161 aaagcttgtt aatcttgcga caaatcttgg agcgatttta gtttttggct ttaactctca
     8221 aatcatctgg gcgctgggat taacgatggc ggcaggaaat atagctggag gatttattgg
     8281 agctaaagtt ggcataagag gcggctcagt tttgattcgt cgggcgtttc tagccgtcac
     8341 taccgcgcta atcatccgtc tgatctatga cacatttctg cggggctgag atctgggata
     8401 gactttgccc cacaacttaa caacaactaa ataagtagga agaaattaaa atggctttag
     8461 tagataactt aaatgagctt ctcgctccag tagtaaaagc atctgggctc ttacttgaag
     8521 agattaaagt gattccagtt ggacgctctc ggatcatctc agtaatagtt gatcacgaag
     8581 agagaaatct aaatcttgat gaggttgctg caagttcaag agcgatatct gaaattctag
     8641 aaaactatag ccagcttggt gataatccat ttacccttga agtcacatcc cctggtgttg
     8701 atcgcccgct ggtaaaggcg tatcaatgga agaaaaatct tggtcgacta atttccatag
     8761 tcaaagtcga tggcgaaaag ctaattggcc gccttaaaag ccttgattta gattcagcat
     8821 tacttgaggt taaaagcgga gaggtaagta ttttatttag cgaaatcaag cgagcaactg
     8881 ttgagattga attcaatcgc aaagatggtg ataagcgatg aatgttgata tgaaagtctt
     8941 agaagctcta accattgaac gtgatattcc acttgatcgc ttgatcactg caattgagat
     9001 tgcagttaag agcgcttata acgagatgga gggagcaaag ccatttgctc atgccaaatt
     9061 agatttagtc acaggggaga taaagattct tatcccagta tttggcccag atggcgagaa
     9121 aatagatgag attagcgatg agcctgaagg ctttagtcgc atcgcatcat ctactgcgcg
     9181 caagattatt aaagagaaga tgcgagaatc aaaagaccta gaaattgttg gtgaatttag
     9241 cgcaactgtt ggcgatattg tctctggagt aattcagcaa ggtcgagatc agaagatggt
     9301 ctatatcgat cttggaagag tagagggaaa aattccacca catgaacaag tgcccggtga
     9361 gatatataag catggtgatc gaatcaaaag ctttgtagtt gaagttaaac aaggccagaa
     9421 gggccctgag gtactacttt ccaggagcca tccagcgcta gtgaaacaat tatttgcact
     9481 tgaagttcca gagattagag atcgcattgt tgaaattatg ggaatagctc gcgaggcagg
     9541 acatcgaagc aaaattgcag ttagagctca ccgcgcaggt gttagtccaa agggcgcgct
     9601 aattggtcca atgggagcaa gagcaagagc tgttatggat gaattacatg gcgagaagat
     9661 agatattgtt gattggtctg atgatccagc aacatatgtt ggtaatgctt tagctccggc
     9721 aagagtctct agcgttgagg tagtaaatat tgatactcgc tctgccaagg tagtagttcc
     9781 tgattaccaa ctatctcttg ccattggaaa agatgggcag aacgcccgtc tggctgctcg
     9841 cctaactggc tggcgcattg atattcactc cgatggggcg acaagtggct cggaggcgag
     9901 cgaagtttcc taaaagtagt actgcggcgg tatggttgcg ccgtgaggat ctccctcaca
     9961 tatttgcaaa gcgaaggcta ggtcgaatag gcaatggcac tctaatgagc acttcacagt
    10021 catgagtagg tcaatttagg cttcgcgttt aaaaagtaga cgaggagcca caaaaggaga
    10081 aaagtggcaa aggtccgcgt ttacgaatta gcaaaagatg tagggatgga tagcaaggat
    10141 gtccttgcaa aactccaaga gatgggcgag tttgttcgct ccgcatcttc aactgttgaa
    10201 gcacctgtgg tgcgaaagtt tctagaaaaa tttcctgcag ttgcagctac acctaagaaa
    10261 gctcctgcca agaaagcccc tgctaaaaaa gctgcagcca agaaagcagc gccagttgag
    10321 agcgttgagc agattgatga aattactcca ccaattactc cagtaattaa agagagcgta
    10381 gaagaaaagc ccgctccact acctgctaaa ccaccagctg ctcctgctgc cagagttgga
    10441 aataatcctt ttgcaacacc atcagctcct cctcgtccac caagaccaca aggtcaaggc
    10501 ggtacaaggc ctggaaataa tccttttgga aatccatcag ctcctcctcg tccaccaaga
    10561 ccacaaggtc aaggcggagc acaacgtccc ggacaagtaa gacctactgg aagtagaccg
    10621 ccaggtgcgc cagcaagagg cggaaatatg gcaggacctc gtccaggttc tgttcgtcca
    10681 ggatttgcag cgcgaccaca acaacgaact gatggttcat ctcgtccgca agatcgcaat
    10741 gcttcatctc catatcgcag ccaaggtggc caaggacaaa gacctggtgg agcaacgggc
    10801 gcgccagcaa gaccaggagc tggaaaacca ggtggcggtt ttgcgccagg aagaccagga
    10861 caacgcggaa gcgctggcgg tgcatttgga aaaaatgctg gaaagaaaaa gaattacaaa
    10921 tcaaagaaga ctcgtcgcga agagatagac aatatgcaag ccccaacact tggtggcgca
    10981 attgttcctc gtggtgatgg aaatactcca gttagattgc gaagaggagc atctttagct
    11041 gactttgccg aaaaaattgg cgcagatcca gctggtttgg tctcagtgct atttcatcta
    11101 ggtgagatgg ttactgcaac tcaatcagtt gatgaagaga ctttgcatgt attaggtagc
    11161 gagtttggct acaaaattac agttgtctcg ccagaggatg aagataaaga gatattagaa
    11221 cagtttgata tcgatctaga tcaagagctc tctgatgtcg atcctgatga tttaagtatc
    11281 aggccgcccg ttgtaacagt aatgggccat gttgatcatg gaaagacaag acttctcgat
    11341 gctattcgcc aaactgaagt agtaaaaggt gaagcaggtg gcattactca acacatcggt
    11401 gcttatcaga ttcatcatga gcatgctgga gttaatcgcg cgattacatt tattgatacc
    11461 ccaggccatg aagccttcac cgctatgcgt gctcgtggag caaaggtgac agatattgca
    11521 gttcttgttg ttgccgcaga tgatggagtt atgcctcaga caattgaggc tttaaatcac
    11581 gctcaagcag caaatgttcc aattgttgtt gcggtcaata aggttgataa agagggagca
    11641 aatcccgggc gaattcgtca gcagttaact gaatataact tggttgctga agagtatggt
    11701 ggagatacgc tatttgttga tgtctccgca gctaaagtca ctggtattga tcaattagtt
    11761 gaagcaattt tgttaactgc agatgctgca attgatttaa gagcaatcca tgaagatgat
    11821 gctagaggtg tagcaattga ggctcatcta gatcgtggcc gtggtccagt tgcaacagtt
    11881 ctagtccagc gcggaacgct aagtgttgga gatgcaatag ttgcaggaag tgcttttggt
    11941 cgagtcagag cgatgatgga tgagtttgga caagaagttg aagtagcagg tccatcaaga
    12001 ccagttcaag ttcttggatt tacctcggtg ccaagtgctg gcgatagctt tatcgttgca
    12061 cctgatgatc gaactgcaag acaaattgca gaaaaacgtc aggccggaga gcgccatgcc
    12121 gcacttgcta aggcaagaaa gaaagtttcc ctagaagact tccttgagca atccaaggtc
    12181 acaactctta atctcattct taaaggcgat gtcagcggat cagttgaagc acttgaagat
    12241 gctttggttc aattagatgt cggcagtgaa gttgatttga gagttattca tagaggcgtt
    12301 ggcgctatca ctaagagcga tgtcactctt gcttcagcat cgggtgcggt aattgttggc
    12361 ttcaatgtta agccagaggc ccatactgca atcttcgctg atgaagaagg cgttgaagtt
    12421 cgattctatt ccgttatcta taaagcaatt gaggagatcg aagctgcgct taaaggacta
    12481 ctcaagcctg aatatgaaga agcagtcttg ggtcgcgctg aagtgcgtga agtctttaag
    12541 tccagcaagt ttggaactat cgctggatct ctggtttcaa gtggcctgat tagaagaaat
    12601 gcgaaggcaa gagtgctacg cgataacgtg gtaatcggcg ataacctaac tatcgaatct
    12661 ttaaagcgat ttaaagatga tgcaactgaa gttcgtgagg gctttgagtg cggaattggt
    12721 gtgggtaatt tcaaagatat tcaaatcgga gatgtatttg aagcatttga gatgcgcgag
    12781 aaaaagcgag cataagacat gggaaagtca catcgggctg caaaagttgc tgaccggatc
    12841 aaggttgtag ttgcctcagc tcttgagagc aaggtgagag atccgcgcct tggttttgtc
    12901 acaattaccg atgttcgagt aactggcgac ctacaacaag cctctatctt ctataccgtt
    12961 ttaggcgatg atgaagctcg cctaaacact gccaaagtat tagaaagtgc caaggggcta
    13021 ttaagaactg aagttggtag agaacttggc accagaatta ctccttccct taccttcttt
    13081 gcagatgcct taggtgagaa tgctaagaac tttgaggatt tacttgatca ggtcagaaaa
    13141 agtgatgaag agattgcaaa tcttcgatct ggcgcagagc cgataagcgg agatgatcct
    13201 tatcgccaga gccgcccaaa aaactatctc tctggtgaat agatgatttt gcaagatggc
    13261 tttgtcatca ttgataagcc agctggaatc accagccatg atgtagtggc aaagttaaga
    13321 aaaactttag gaacaaagca ggtcggccat gccgggacct tagatccgat ggcaacagga
    13381 gttcttgttc ttggggtaaa taaagcaaca aagtttctgc aatatataac tcaaggaaaa
    13441 aaggcctatc acgctcagat tcgattaggc caggcaactt ctagcgatga tgcgcaaggt
    13501 gaaattactt caacttctga tacttcaaat gttagcgatc aagaaattag agatttcctt
    13561 gctactcaaa ttggaagcat tatgcaggtt ccaactaaag tctctgcgat aaaaatcaat
    13621 ggccagagag cctatgactt agtcagagag ggaaaagagg tagacattcc tgctcggcag
    13681 atagatatct ataacttaga tgtaatcgaa atcaaacgtg gagaatttct agatattgaa
    13741 attaatgttg agtgctctgc aggaacttat attcgatcaa ttgcaagaga tttaggaagc
    13801 tcacttggag tcggaggaca tctgatttct cttcgaagga gcctagttgc tccattctcg
    13861 ctaagtgatt gcagtagcct tgaatctcca gagataaggc ctcttgctag cgagatatct
    13921 aaagttatga gtgtaagaaa tgtagatctg cttgaggtta aagagttgag ttttggtcgt
    13981 tcactgagcg cgtcaaatag tgatggccca gttgtggcca ttgctcctga tggaaaggtt
    14041 gccgcaatat tggaaaatcg ggaacatggc gcacaacctg tggcggtctt tatttcatga
    14101 aagaattaaa gctatgagta gcgctgaagt aacttggcat ggtgccccgc ttctaggctc
    14161 agcccttgct atcggaattt ttgacggcgt gcacattggc catcaagtaa ttcttcaaga
    14221 gggggtaaga gaaaacctgc cagtagttgc actcaccttt gatcctcatc caacatcagt
    14281 atttgctcca gatcgagttc caactcggct tctaacgcta aataatcgca tcgcccagtt
    14341 atcaatgcat ggggcaagta gcgttgctgt aattaagttt agctctcaat tctcacaatt
    14401 aactccagat caattcatta accaaatatt aaagccaata tttgatcctg ctttagtttt
    14461 agtgggggag aacttcacat ttggagcaaa agctgccgga aatgttgagt atttaaaagc
    14521 taattcacct tttaaggtaa aggcaattcc actttatagc catggaaata ttaaagtctc
    14581 ctctacgcga attcgagaag caattaaggc aggaaatatt gagattgcta atcaattgct
    14641 aacaagaccc catcaattag tgggcccagt tattcatggc gaaaaacgtg gcagaactat
    14701 tggatatcca actgcaaata ttggtctaga ttcttatgcc acaattccat ctgatggggt
    14761 ttatgccggc tggttatcag ttggttctca tcgctggagc gcagcaattt ctattggaac
    14821 taatcccaca tttgatggcg tgcgcggaag acaagttgag gcctacgcac ttgatcgcga
    14881 tgatctagat ctctatgacc aagaggcaaa agttgaattc ggttggagac ttagagatac
    14941 catcaaattt ggctcactag atgagctctt ggttcagatg aaaatagatt gcgatgaagc
    15001 taggaaactg acttcccttt aggcgtattt tcgcctcgat tttagatctt taagcagcgc
    15061 tggtagattt acgcccctca agcgagaaag tgtgcgctcg tgtattagac cgagagccct
    15121 cgtgaaagat gtaaaggaag aaaaaaaatg gcattaaaac ctgccgagaa gaaagaaatt
    15181 attaccaagt atggcgcctc tgcatctgac acaggaagcc ctgaatcaca ggttgcgctt
    15241 ttatcacgga gaattgaaga agtaactgag cacctacaga caaatcagca tgatcaccac
    15301 aaccgtcgtg gtctactgct tctagtaggt cgccgtagaa gaattttgca ataccttgca
    15361 aagacagata ttgcgcgata ccgcgcgatc atcgacaaac tcggcatccg ccgttagttt
    15421 gctgaacaat taaataatca cccagcgcat cgctagaggt ttggtcctcg gtggtggctt
    15481 acggatttaa acaagaatgc cgttggcttc gatcgaagat ccattctcta taaatatgcg
    15541 ttcgggtgtt agagaatagg agagacccat ggagggtcaa gacgttaagt ctgccgctgt
    15601 cacaattgac aacggaaaat ttggaaaacg agaaattcgt ttcgaaactg gtcgcctagc
    15661 gcgccaagct gcaggaactg cagttgttta tctagatgat gaaactatgt tgctatcagc
    15721 aactactgct tcaaagtcac ctaaagatca attcgacttc ttcccattaa cagttgatgt
    15781 ggaagagcgt atgtatgcag ttggaaagat tccaggatca tttttccgca gagaaggtcg
    15841 tccttccgag gatgcaattc ttacctgccg cttaattgat cgtccgctac gcccttcatt
    15901 cattaagggg ctgcgtaatg aagttcagat cgtagtgact gttatggccc ttgatcagaa
    15961 tcatatgtat gacgtgattg ctattaatgc ggcatctatg tcaacgcaat tagcaggact
    16021 gccattctca ggtccaattg gtggcgtgcg tatcgcactt atcgatggac aatgggttgc
    16081 tttcccaaac catagcgatc tagaaaatgc tgtttttgat atggtcgttg ctggaagaat
    16141 tgctggcgat gatgttgcaa ttatgatggt tgaagcagaa gcaacagtta agaccattga
    16201 tttgattggc tctggggcta gcgctccaac tgaggaaatc gttggtcagg gtcttgaggc
    16261 ttcaaagcca tttattcgtc agctctgcca agcacaaatc gaactagcaa aagttgctgc
    16321 aaagccgact gctgagttcc cagtatttct cgattatcaa gatgatgcat ttgcagctgt
    16381 tgagaaagca gcaaagaaag agcttgatgc tgcgctaaag atttcaggca agcaagagcg
    16441 tgaaactgag attgagagac tatctactga agttaagcaa tcacttgctg agagctttgt
    16501 tggtcgggag aaagaaattc ctgcagcatt tcgctcacta actaagaagt tggttcgcca
    16561 gcgcgtgctt cgtgaaaaga ttcgtatcga tggacgagga cttcgcgaca tcagaccact
    16621 gattgctgaa gttgaggtag ttccaagagt tcatggctct gcaatatttg aaagaggcga
    16681 gacccagatt ctgggaatca caactttaaa tatgcttaag atggagcaac agctagatac
    16741 gctcaatcct gaagaccata agcgttatat gcataactac aacttcccgc cttactcaac
    16801 tggtgagact ggtcgagtgg gatcacctaa gagacgagaa attggtcatg gagcacttgc
    16861 tgagcgagcc ctccttccag ttcttcctgg gcgccaagag tttccatatg cgatcagaca
    16921 agtatctgaa gcacttggtt caaatgggtc cacatcaatg ggatcagtat gtgcatcaac
    16981 tctctcgcta ttaaatgcag gtgttccgct aaaggctcca gttgctggaa ttgcaatggg
    17041 cttaatttcc gatgatgttg atggcaaagt ggaatatgtt gccctgacag atattttggg
    17101 agcagaagat gctttcggcg atatggactt taaagttgct ggaacaaaag actttgtaac
    17161 agctcttcag ctagatacta agttagatgg aattccagct agcgttttat cagctgctct
    17221 tttgcaagca aaagaagctc gtcttgcaat cttaaaggtg atgaatgaag ctattgatcg
    17281 cccagatgat atgaaccctc ttgctccaag aatcatctca attaagatcc cagtggatca
    17341 gattggtgct gttattggac ctaagggcaa agtcatcaat cagattcaag aggagaccgg
    17401 cgctgaaatc tctattgaag atgatggaac aatctttatt ggtgccacaa atggcacagc
    17461 agcagatgct gctaaagctg cgattctctc aattgccgat ccaaagcttc ctgaaattgg
    17521 agagcgatat aacggaacaa tcgtcaagct tgcaaccttc ggagctttcg taaatctcct
    17581 tccaggaaaa gatggcttgc tacacatctc tcagattcga aagatgcatg gcggaaagcg
    17641 cattgaaaac cttgaagatg ttatgaatgt tggcgataag gtggaagttg aaattggtga
    17701 gattgaccca aagggcaagc tctcgctaat tccagtttta gatgagagcc agaagtcaga
    17761 tactgcttca gagtaagtga aattaaatga ctgtgcgtag gaccgttctt gctagcggcc
    17821 tacgcatcgt cactgaagaa attcctacgg tccgctcagc cgcctttgga atctgggtca
    17881 atgttggatc tcgcgatgag agcctaaaag ttgcgggagc atcacacttt ctagaacatc
    17941 ttttattcaa gggaacaaag cggcgtagcg cgcttgaaat ttcatcctca attgaagccg
    18001 tcggcggcga aatgaatgct ttcacatcca aggaatacac ctgcttttat gccagggtaa
    18061 ttgataagga tcttccatta gctattgatg ttatttcaga tctgattacc tcatctcttg
    18121 gtaaatcaag tgatgtggag gcagagagaa aagtcgtact agaggagatc tctatgcgcg
    18181 atgatgatcc ggcagattta gttcatgagt tatttgctga gactttctat ggcgacacca
    18241 cacttgggcg ctcaatactt ggcactacta aatcaataaa gtcactttca agaagcgcag
    18301 tatttaacta ctataaaaag cgctatctac cagaagatat cgtggttgca gttgctggaa
    18361 atatcaggca tcagcaggtc gtagatcaga tcattgcagc gctttcaagg gatgagtttc
    18421 tcgatcgaac tgagaagaaa tttaatctaa gaccaagcca ggaatttagt agaaagccaa
    18481 agagaaatgt tgggctgata aatagaaaga cagagcaggc ccacatagtt cttggaatgc
    18541 caggagtaaa tcgggacgat aaaagacgtt ttgccatggg agttttatct gccgcacttg
    18601 gcgggggaat gtcttctagg ctatttcaag aaattagaga aaagcgcggt cttgcttact
    18661 ccgtctactc atatgctcaa cagtttgccg gaagtggctt tcttgccctc tatgccggat
    18721 ctacgccatc aaaggctgtt gaagtaataa agataatgcg cggagttttg gaagatgttg
    18781 caagtaatgg actaacaagt gaggaattaa tcaaggctca aggagcagtt agtggctcac
    18841 tagttttagg ccaggaagat acgggttctc gaatgagccg gattggaaag agcgagctga
    18901 tctatggcca agtcttaagc tttgatgaga tccttcgaga gatttcagcc gttgatagcg
    18961 cagccattgc aaagctagca tctgaggtct tgggttctgc gccgagcctt gcgattgtgg
    19021 gaccatttgc caagcgttca atctttgaaa aggctatgaa gtagaaggtg gagaagcgat
    19081 gaaaattaaa gtcgccattc ttggcgctaa gggtcgtatg ggtagcgaga gcgttaaagc
    19141 tatctctgct gcagatgatt tggaactcgt tgcatccctt gatcttggcg attcattaga
    19201 tttgctaaag agctctggcg ctgaagttgt attggaattt actcatcctg attcggtaat
    19261 gaaaaatcta gagtttgcaa tcagtaacgg tattcatgta gtagttggaa ctactggttt
    19321 tgatagcaag agaatcgaag aagtcaaagc tctgttagct aaaaatcctc aagttggagc
    19381 aataattgca ccaaactttg gcctaggggc agtattaatg atgcagtttg ctgcaaaggc
    19441 tgccacttat tttgaatcag ttgagattat tgaactacat catcccgaaa aggcagatgc
    19501 cccttctgga actgcaacta gaactgcaga gttaatctct gatgccagag cgaaagccaa
    19561 gaaaagcgca atgcctgata agactagtag cggacttgat ggagcaagag gagccaaggt
    19621 tggagatgtt ccaattcatt cggtacgcct aagaggttta gttgctcatc aagaggtcct
    19681 acttggcgat caaggcgaga ctctctcgat tagacatgac tcaatagata gaagtggatt
    19741 tatgcctggg gtactgcttg ccataagaag cgtatcgaca agacctggtt taacctttgg
    19801 ccttgagaat ctgatggata tctaggagga aaatgtgagc acttcaataa caatgtatag
    19861 cgcagattgg tgtggagatt gcgtcagatc taaaagattg cttgattcac ttcaagttag
    19921 ttatgaaatc attgatgtcg aatcaattcc tggagcctct gagaaagtta ttgaaataaa
    19981 cggcggaaag cgttctatcc cagtaattct ctttcctgat gggacccatc taactgaacc
    20041 ttctgatatt gatctaaaag caaagcttga agctttaaag attatctaga ggttataaac
    20101 tagggcagag cttgccgcag caagcaacat cattattgga aagtttgctt taaaacttaa
    20161 tgcaatagca gcaaccagta gtcctacaag cctgtgatca attacaacct gttgctcaat
    20221 tgttagcgct tgaacagcga ctaatgagct aagaagtgcg ataggtataa gtgtattaat
    20281 cctttgaatc gctggtctat taagaagaga ttctgggatg cgatagccca gatacttcaa
    20341 taaaaagcag atcagagacg aaactaaggt tgcaatccat atctgactca tcgagctgcc
    20401 ctacttccaa aaattactgc caatagcgct gttgcaatta ttggaactcc agctggagca
    20461 aatggaacta ataaaagcgc caaaagcgca gcagatatgg caagtactcg agttttattg
    20521 tcagtaagtc ttggccagag gagtccaaga aatgctgccg gaactactgc atcaagaccc
    20581 caggctgcag gatctccaat tgctccagcg ccgatggctc caagaagagt aaagagattc
    20641 cagaagaaat aaactccaat tccagttgcc cagaagccat aacgcgcagc ctcatcacct
    20701 cgcccctctt ggctaattgc aacacctgtt gattcatcaa ttgttaattg cgcgccaatc
    20761 aaacgcttaa aacctcttaa tctcagtaga ggtgccatct ttaatccata gagaccattt
    20821 cgaaaaccga gaagagatcc agtagcaatt gcgctaattg cagatccacc tgagcccata
    20881 actccaacaa ctgcaaattg agatgcaccg gaaaataaga gaagagataa gagacaggtt
    20941 tgaagaatcg aaaatccagc cgcgattgag gctgctccaa aggctgcgcc atatgtgccc
    21001 actggaattg ccacagccag cgagtcggat aggacgcggc gcctattaaa gctcggagga
    21061 tttaatgaca cgcgctcatt ctgccgtaga aaatcccagc cttgtgccag agagtagata
    21121 aggtcgcgca cgtgagccag ccaagccatc cagatattgt ctttagagat gatgtgacag
    21181 tagagctagt gaaatctagc gctagtgatg ctgatgtcat ttgggcggcg cgagtctcaa
    21241 cagcaggtga gaattcttta gaggaagtaa acgcagatcc tgctcgctca actggattga
    21301 ttaattattt ggctagagag cgccatggct ctccttttga acatacctca atgacttttt
    21361 ttattagcgc cccgatattt gtatttagag agtttatgcg ccatcgaatc gcctcatata
    21421 acgaggagag tggtcgctat cgcgaactgc ttccagtttt ctatgttcca aataaagcta
    21481 gaaaattagt tcaaactgga aagactggtc actatgtatt tgttgatggt agtgatgagc
    21541 agtatgagat atcagtagct gcgatgaaag aaagttatga actttgttat ttgaattata
    21601 agaagatgct agatgcaggg gtggcaaagg aagttgccag agcagtgctt ccggttgcgc
    21661 tctattcatc tatgtatgtc acgatgaatg ctcgagcgct tatgaatttt ctctcgttaa
    21721 gaacagcccg agaaggatct cactttccta gctatccgca gcgggagata gagatggtgg
    21781 cagagaagat ggaggagcac tttgcccgcc tgatgccgat tacctatgct gcctttgaaa
    21841 agtcggggcg cgtcgcgcca tagccggtaa gtagtagcct tggcgcatgt cgattcaggc
    21901 gccatttggc agattgttga ccgcaatggt cacccctttt aaagatgatc tatcaattga
    21961 ttgggcaagc gttgaaaaag tagccgcaca tttagtttca acgggtcatg atggaattgt
    22021 tctaaatggc acaacagggg aggcgccaac tactagcgat gaagagaaag ttgaaataat
    22081 caaggtagtt cgcaaagtag ttggatcaaa tataaagatt gttgcagggg ctggaaataa
    22141 cgaaacttct cactcactcg agcaggcaag gcaggcagct aaggctggag ctgatggact
    22201 tttagtagtg actccttatt acaacaaacc accgcaggct ggaatcgcag ctcactttga
    22261 agctatggca ggcgctactg atttgccaat aatgctctat gacatcccac atagaactgg
    22321 tgttgcaatt gagccagata cgattgttaa gttagcggca aatcccaaaa ttgcagctct
    22381 taaagatgca aagggagata tcgcttcaac ttcttgggta attaagagat gcgctatacc
    22441 ggtttactca ggtgatgaca ttttaaatct tccactactt tcagttggag cggtgggctt
    22501 tgtctctgtc tgcggccata gcgtcggatc agagttgaaa cagatgctta atgctttctt
    22561 ttcaggtgat gtcgcaaaag ctcttgaaat tcatcaaaga ttattgccgg tatataccgg
    22621 aacatttaga acccaaggag caattcttac taaagccgct cttgatttaa tgggcttaag
    22681 tggtggacga gttcgcctgc cgttagttga tgcaaccaag gctcagatag accaactacg
    22741 agaagatctt cgcgctggcg gcgtgaaagt taagtaatga atcatccgca tcccgatcta
    22801 gggttgccgc caaaacttgc cgccaaaact ctaaggattg ttgccttagg tggccttgga
    22861 gaaattggtc gcaacatgac cgtttttgaa tatgaaggcc aattattaat cgttgactgt
    22921 ggcgttttat ttccaagtga aactcagcca ggagttgatt taatcctgcc agatttttct
    22981 tatctaaagg agcgaatggc tgatatcgcg gccgtttttc taactcacgg tcatgaggat
    23041 cacataaccg cacttggcta cctacttcgt gagcgagaag acattcaagt aattggcagc
    23101 gctttaactc ttgctttctt aaagggaaag ttaagagatc atcgaatctc accaattatg
    23161 gttgaagtta aagaaggtca tagcagaaaa gttggacctt ttgatttgga atttattgca
    23221 gtaaaccact caattcctga tgcgttagca gttgcaataa agactcctgc cggggtagtg
    23281 cttcatactg gcgattttaa aatggatcaa cttcctctag atggaagagt tactgattta
    23341 gctcgctttg catcccttgg ccaatctggc gttgatttat ttatggttga ttcaactaat
    23401 gctgatattc caggatttac cacatctgag cgagaaatta tgccggtatt agagcgagtt
    23461 attgatggca caaaggcgag agtaattgtc gcctcctttg catcacatgt tcatcgtatc
    23521 cagcaggtga tagatattgc ctatagcaag gggcgaaaag ttgcctttgt tggacgctcg
    23581 atgattagaa atatgacgct cgctgcagag atgggatatc tacatatccc accgaatact
    23641 ttgctagagg ctgatgaaat tgaaagcgct ggagataaag ttgtactgat ttgcacagga
    23701 tctcaaggtg agccactggc agctttatca agaatggcaa atggcgatca tcaaataaga
    23761 gtaggaagag atgacactgt aattcttgca tcatctctga ttccaggaaa tgagaattcg
    23821 gtctatcgag taataaatga attaacaaga tttggcgctc gcgttgtaca taaaggcaat
    23881 gcgctagtgc atgtttcagg tcacgcagct gcaggggagt tgctctattg ctataacgtg
    23941 gtcaagccaa aaaacgttat gccggttcac ggtgagtggc gacatatgac ggccaatgct
    24001 gagcttgcaa tctcaacagg agttaagcca agcaatgtag tagtggttga taatggagtc
    24061 attgtcgatt taagcgaagg aagagcaaag gtagttggcg cagttccagt tggctatgtt
    24121 tatgttgatg gccagagcat tggagatatc accgaggcat cgataaagga tcgtttgatt
    24181 cttggagaag aaggttttat ctcagtaatt gtcgttatcg attcacagag tggaaaaata
    24241 gttgctggtc ctgatatcca tgcacgcggt tttgcggaag atcttgagct cttcaatgaa
    24301 gtaaagggtc gtattgagcg agccctgact ggagcagtag ctggtggaat taatggaact
    24361 catcaactct cgcaagtagt tagaaagaca gtgggaagtt gggtagggga agaacatcgc
    24421 cgtcgcccga tgattgtgcc tgttgtaatt gaggtttagc ggcgcgcctg acctttaaga
    24481 aaattaattt cgaatacgat ttttcgttgt ggcaaagaaa aaatccgcta ggaaagcaag
    24541 tggcctactc tcggctatcg ctactggctt gggatttatc tggagcgcta ttgccaaagc
    24601 tctcggcgca agcatcaggt ttattactcg tggggctaaa gatttagaac ctgagcatag
    24661 acgcgatggt attggtttaa ttcttttaat tgttgcccta attgctgcag caacttcttg
    24721 gatgcaatta gataatgctt ttggcagagc tgcatatgcc ttcttctacg gaggatttgg
    24781 cagggttgca atacttacac ctattctctt tggctacttt gcctttcgcc tatttcgcac
    24841 cccacaagat aagggcgcaa ctggtcgaat aacaattggc tcactcctac ttgttctaag
    24901 ttcaactggc ctaatccaca tattaaatcc ttcatcgatt aatactggcg ctactgcaat
    24961 gcgcgagggc gggggatgga ttggctatgg ggtctcaacg cctcttgttg cgctacttac
    25021 cgatatgctc gcaattccaa ttcttattct gaccttaata tttggcgcac ttgttattac
    25081 agcaactccg ttttcaaagg taattgattt gtttaagaga cttatctctg gcgcaagcaa
    25141 taaagttagt ggcgcacttg atcaaagacg agagcgaaaa ctggagcgag aagagtttga
    25201 aattgctgat acgccgccat ttgaaactcc cttagttcaa gattttagta atgcccaaga
    25261 agaggatctc gaggaagaaa tagatgaaga gagctttgat gaagaattta cagttgagat
    25321 tcctaagcct gaggtaagtg caaagcctgc aagacccgtt caattactgc tttcctcaac
    25381 tgattcctat gagctcccct ctatggatct acttcgaact accccatcta gtaagacaaa
    25441 gaccaaggca aatgatcaaa tagttcaatc tttaactgaa gtctttgctc aatttgaaat
    25501 tgactgtgaa gtaactggct ttatgcgagg acctaccgta acgcgctatg aagtcgagct
    25561 tggctccggg gttaaggttg aggcaattac agctttaagt aagaacatcg cctatgcggt
    25621 tgcaagtagc gatgtaagaa tcctctcgcc aattccagga aaatctgcag ttggtatcga
    25681 gattccaaat agcgatagag agatagtttc tctaggcgat gtgcttcgct ccagcgttgc
    25741 aggaaatgac caccacccaa tggtgatagc tcttggtaaa gatgtagagg gtagttttat
    25801 ctgcgcaaat cttgccaaaa tgccacatct acttgtggcg ggcgcaactg gcgctggtaa
    25861 atcatcaatg attaattcat tggttacctc agttttaatg agagcaaccc cgaatgatgt
    25921 tcgcttaatt ctcatagatc caaaacgcgt tgaacttaac tcttatgaag gcatcccaca
    25981 tctaattgcg ccaattatca ccaaccctaa aaaagcagca gaggcacttg cttgggtagt
    26041 gcgtgagatg gatatgcgat acgacgatct ttcaacatat ggctttaggc atatcgatga
    26101 ctttaataag gcagtcagag ctggaaaagt tattgctaaa gagggtgccg acgctgcact
    26161 tgagccctat ccatatctat tagtagtaat tgatgagttg gcagatctaa tgatggttgc
    26221 tgccagagac gttgaagatt gcatcgtgag aattactcag cttgcaagag cagccggcat
    26281 tcatcttgtt atcgctacac aacggcctag cgttgatatt gtcacaggtt taattaaagc
    26341 taatgttcct tcacgtcttt ctttcgccac ctcatcactt gctgactcca gggttattct
    26401 tgatactcca ggtgctgaaa aacttgtcgg acaaggcgat gccctatttt tgccaatggg
    26461 agcaagcaaa ccgatgcgaa ttcaaggcgc gtatgtatca gagcgagaaa ttgaagcggt
    26521 agtaagccat gtgaagtccc agctaaagcc ggtgtatcga gaagatattt taaaaccagt
    26581 agttgcaacc agaggctctg atgaagaaat tggtgatgac aaagagttac ttatccaagc
    26641 aatcgagtta gttgttagca ctcaatttgg atcaacttct atgttgcagc gaaaattaag
    26701 aattggtttt gctaaagctg gacgtctgat ggatttgatg gagagcagag gaattgtggg
    26761 accatctgaa ggttctaagg ctcgagtagt tttagttaag caggaagacc tggcctctgt
    26821 ccttgaggta attcaggggt gaatctttag gggcgatatt ccagctccct tgctcaattg
    26881 agcaccgctt caagatttag gttgtcgctt gcctgcttct gcttctacaa tttggcctcc
    26941 ccacaagtga tttagaggct ttcatgtcgg ttggttcaac gctaaaagag atgcgcaagt
    27001 cggcaggttt tactgtcgag caacttgcca agcgctcgcg tatcccggca tcggtgattg
    27061 aggatttaga gaaagataat ttcctaactg caggtggccc aacttatgct cgtggccata
    27121 ttaaaacaat tgcgcgaatc tgtggagttg gcgatagtga tgtgcttatt aaatttgaat
    27181 ctcaaacaat tcctttaagt aaatcgctta gagatctatt gaatgaaaat agcgttacaa
    27241 agcagaagaa agagagaaag ccgatatctt ggaagacgct aactggtgca gcagctggct
    27301 tatttatctt tgcacttgtt ggaacagcaa ttatttccat tgaggattca ccagagcaaa
    27361 gcggcatcac cgcttcaagt aacggtaaag atgagagcca acctagcgct tcaattagag
    27421 atggcgttga ggtaagactt aaggcagtga atggactttc atgggttgca gtaagtgata
    27481 gcactggcac cacacaattt agtggacgaa ttcgccaggg cgaggagaga gtatttagcg
    27541 ataatcaact gctctatcta gtcattggaa atgcaggcgc gattaacctg acggtaaatg
    27601 gtgaagatat tggagttgct ggcgcagttg gcgaagtttt acgtctggaa ttcggtcctc
    27661 aagcatctaa tcaaggataa gttctacttc ttctctgtag aataactagg tgtccaagcc
    27721 taaaagcgtt gccctagtaa ctctaggctg tgcgcgaaat gatgttgatt ctgaagagtt
    27781 agcaggacgt cttgcagctg atggctggca attagttgat gatgtagcaa ctgctgatgt
    27841 ggcgctagta aatacctgcg ggtttatcga atcagctaag aaagactcag tagatgccct
    27901 gattgaggct cactccatga aggccgatgg aacgcttcgg gccgtagttg cagtgggctg
    27961 tatggctgag cgatatggca aagagctggc agaggcgatg ccagaggctg atgcaatttt
    28021 aagttttgat gattatgcag atatctcaag tagattacag aatattcttg ctggaaattc
    28081 aaagccagct cacaccccaa aggatcgtag aagtttaatt ccaatctcac ctgttgcaag
    28141 acaggagatg aaggttgagt taaatacaaa aatggggcaa ggcggcacct ttgcaagaaa
    28201 gagacttgca aactcaccaa tggctccgct aaaaattgct tcgggttgtg atcgcaggtg
    28261 ttccttctgc gctatcccgc aatttagagg atcttttgtt tcaagaaccc cagatgagat
    28321 tattaccgaa gctagatggc tggcagaaaa tggtgtcagc gaactcttct tagttagcga
    28381 aaacaccacc tcttatggaa aagatcttgg tgatttaaag gcaatggaga aattactgcc
    28441 agccctttcc aaaatagatg gaatcgagcg catcagagct tcatatctgc agccagccga
    28501 aattagaccg acgctactgc aagccttgat tgctaccgag aaagttgttc cctactttga
    28561 catctcattt caacacgcct caggcactct tcttagaagg atgcgccgtt ttggagatgg
    28621 cgagaagttc ctacacttat taactcagat aagagcactt agcccagagg ctggaattcg
    28681 ctccaacttt attgtgggat ttcctggtga gagtcagggc gagtatcaag agctagtgga
    28741 ctttgttaat tcatcaaatc ttgatgcaat tggaatcttt gcctactctg atgaagataa
    28801 aacggaagcc ctgacactag atggaaagtt ggatccgcaa gttattagtg agcgaaccag
    28861 tgagttaacg atgattgctg aagagttagt aatgcagcga gcaacagatc gaattggaca
    28921 gagagttaga gttctaattg aagatgaaga actccaagag ggaagagctg agcaccaggg
    28981 gccagaggtt gattccagca cctttgtaac cattgcagga aggccaagtg ctggttataa
    29041 agtcggtcag tatgtcgatg ccatcattac cgatacttca ggggcggatt taattgccca
    29101 gagcctatga accttcctaa cgccttaaca attgcccgga tcgcagctct tccattttgt
    29161 gcttgggcgc tatttaaaaa tgggggagat gatccaaact ggcagataat tgcttggttc
    29221 tccttcttta tagttggaat gaccgatgtt ctagatggga ttattgcaag acgttggaat
    29281 caagtttcat cctttgggat tattttagat ccgattgctg ataaagcttt tatcgctact
    29341 gcattaattg gactttcgat tctaggcaag atgccttggt gggtcacagt attaattctt
    29401 acccgagaag ttggaattac agttctgcgt ctcctagtaa ttaagaaaaa agtaataagc
    29461 gccagtaaag gcggaaagat aaagacactt ttgcagaact tctcagtagg tttctacata
    29521 cttcctctgc cagaggcgct tcatactcct agagatatat tgctagctat tgccatcgcc
    29581 cttaccttca tcaccggcta tcaatatcta aaagatgtag tgaaatcaaa gccatgagtg
    29641 atcttgcagc aacggtaatt gatctactaa gaaagcgcag cgagagcatc tcctgcgcag
    29701 agtcaataac tggcggggca atcacatcga aacttgtttc ggttgctgga gcttcagatg
    29761 ttttactagg ctcaatcgtt gcttattcaa aagagatcaa gatgagtcag ctaggtttaa
    29821 gtgcagagtt aattgattcc aaaggtctgg tttctcaaga ggttgcaatt gagatggcca
    29881 agggggccag gcagagactt ggaagtaatt gggcgatctc ctcaaccggc tctgctgggc
    29941 caacagctct agatggcagc actcctggtg agatctggat tgcaattctg ggccctgata
    30001 ggcaagaaag tgtgaaatta tccttaaatg gcgctcgcca ggaggtcata aacggcgcgg
    30061 tagagagcgc attaaccctg ctggagcgta tccttaggtc ctaggaattt gttcaatcat
    30121 tctttcaatt aggaggcata gcaatggagt tactacgtag ccatattggt caatgccttc
    30181 gcacagagcg catctcacag agtagaactc taagagatgt tgctaaggct gctcgagtct
    30241 ctctcggata tttatctgag gtagagcgcg gccagaaaga agcttcatca gagctactta
    30301 atgcaatttg tatcgcactt gatttgtcgc tatcaaaagt actagttgag gtaactgctc
    30361 aagttaaact caacgagacc ccagtactta gcgtcgttga taccaacgct gcttaactag
    30421 aggggaagaa taaggctgtg agagatcaga gcgcgcgctc gacctatcaa caccaatcaa
    30481 taattcatcg tcgcacaaga agcgccataa tggcagcagc gaaagagctc ttggcccaaa
    30541 gtggtatctc tggaactaac atgattgaaa ttgccgatag agctcaagtt tcaagagcct
    30601 cgctatataa ccactttaga gataaacatg aagtcttttt agctttggtg gaaagtgagc
    30661 tggagcgaat ctcaactctt gcaatgattg cccaatcacg atcagaggcg ctctatttaa
    30721 tctcctgcga gatttcaaat catccagggc ttaagagcgc actagctagt gatggcgaga
    30781 ttatggcaaa tgcgttaact gcaagagaac ataaaatctg ggttgagatc tatgcccagc
    30841 tctcaaaaat atttgcaaca gatgtagttg gagttggatt aattcttcgc tggctgatgg
    30901 ggcaggtaac tgccccgctc tctgatgagc actctaaaga gcaggcagag cgtttagcat
    30961 ccattcttta aagtaagtaa tggctttatg agaatcaccg aacttcgtag aaggttaact
    31021 gctcacttcg gccctgattg ggccccttcc tattccaaag attttgtaat ggcagaactt
    31081 ggcggtcaga gcgtggatca ggcccttgaa atgggatttg atccagctga tatctggcgg
    31141 gcggttgtga aaaataaccc agatatttca agcgaactta aataagctca tatctcatag
    31201 caacagctcc cttagggcga taagttgctc gaggttgggc tttaggaaaa ccaccagtaa
    31261 gtgaaagtct ggctctcctt gcaatctcta aaataattat tcgtccctca agcagggcaa
    31321 attgatctcc taggcacttt ctactaccag ctaagaatgg gaaataagcc ccgcgtggca
    31381 agctcttttc aaagtcattg ctccatcgct ctggaataaa ttcatttgga gatggaaaat
    31441 acttttcatc cctttgagtg acatattgac taactaaaac atgggcccct tttggaatct
    31501 ctctgccacc aagtgttaca tcctctagcg cccttcttgg tgagatccag actgggggag
    31561 caagccttaa aacctcgcta aaaatagctc cagatagggg agctgcgaga atgactctgg
    31621 caaagtcttc atcgccttct ctagcaaaaa cttcctctgc ctctttggca aggccatccc
    31681 agtacttagg attctggctt agatatgcaa aggtccaagt taaaacattt gcagtagttt
    31741 catggccact taaaattaaa gttagagttt catctgaaat ctctcgagcg cttaacttct
    31801 caccatcgac agtttctgcc tcaagtaaga ctcccattaa gtcatcgcgt ttgactccgc
    31861 ttgcaattcg ctccgtaaca atcctagttg caacgttatg gagctcatta gcagcttttt
    31921 caaactttct aaagtatgga ataggaagat tatcaaggcg atcaaggccc ggaagcatgg
    31981 tgcgctcaat acgatctatt gcaatatgca tcgagtcttg aactcgcttg gtatctgctg
    32041 aaatatctgt gccaaataaa atatctgcaa cgatgtcaaa agttagcgac atcatttcag
    32101 ggccaatggc tacgacctgg ccatcgctcc aatttgagat gtgcttgcga gtgaggttta
    32161 gcatcgtctg ggcataagtt gagatcttgc taatatgaaa tggtgattgc atcaaccttc
    32221 tatggcgaag atggataggt tcttcattag ttaaaaggcc gcttcctaga acttttctca
    32281 tccgatcaaa acccacgccc tttataaagc ttccctgctt gcttaccgtt acctcattga
    32341 ccatctctgg tgagaaggcg gtgataaaga gctggcctgc taaaaagaat gagctgcaat
    32401 cgccatattc ggccttggca tttagtagaa aggctggggt atctttttga aatctaaggg
    32461 ttagtagggg cgatcttggc ccaccaggaa tatttagacc cttcaaatct cgccgcttta
    32521 gaggctttgg catcggggcg tactcagctg gccgtgggct ttgaaagggg gcgagatcaa
    32581 ccatggctcc aaggtagggc tcgcgtgtct taaaagtcat tagaacagat gttcggctag
    32641 acttatcccc ataacagcgg cgagctgtta tacccaacca gagcggttcc acatttccgc
    32701 cgactgcaaa gtcaggccgt cagtggcatt ccgtaccttc tggagccaac tacgagagaa
    32761 gagaggcaag accgtggcta aagaagccaa agatccaaat gaaaaagcaa aaccctctgc
    32821 cgaacggcag aaagccctag ataccgcgct tgctcaaatc gaacgtcagt ttggcaaggg
    32881 ctcagtaatg aagatgagcg atcgaaccgc aactccaatt gaggtaattc caactggatc
    32941 catcgctctt gatatggcct taggaattgg cggcattcca cgtggccgcg ttgtagaaat
    33001 ctttggacca gaatcatcag gtaagacaac gctaactctg catgcaattg ctaatgcaca
    33061 gaaaaatggc ggtattgcag cttttatcga tgctgagcat gcctttgatt ctgaatatgc
    33121 aaagaaactt ggagttgata ttgatgcgct tctagtttct cagcctgata ctggcgagca
    33181 agcacttgaa attatggaca tgttagttcg ctctggagca cttgatttaa ttgtggttga
    33241 ctccgttgca gcacttgttc ctcgcgctga aattgagggc gaaatgggag attcccatat
    33301 gggacttcag gctcgcttga tgtcacaggc cttaagaaag ataactggcg cgctaagtca
    33361 gaccaagaca acagctatct ttattaatca gctccgcgac aagatcggtg ttttcttcgg
    33421 ctctcctgaa accacaactg gtggaaaagc gcttaagttt tatgcctcag ttcgtatgga
    33481 tattcgccgt attgaaactc taaaagatgg acaagaagcg gttggaaatc gtacccgcgt
    33541 caaagttgtg aaaaataaga tggctccgcc atttaagcag gcagagtttg acatcatcta
    33601 cggtattgga atttctagag agggaagcct tctagatatg ggcgtagatc ttggaattgt
    33661 gaagaaatca ggagcttggt ttacctatga aggagatcaa ctaggccagg gtaaagagaa
    33721 ctctcgccaa ttcctgcttg ataatcctgc tctagctaat gaaatcgaag gaaagatccg
    33781 ggctcacttt gtcgcgagcg atatcgatcc agctctagtt gctgcaatcg atgaagccgc
    33841 agctgatgtc gagttctaat tcagtagaag ttgagagcga cccctaccaa gtagctcaga
    33901 ccattgccct tatggcactt ggtcgaaggg caaaaagtag gggggagctc tttaagcttc
    33961 tcaaatctcg cggcattgaa gaagaggttg caaacgcagt tctctttcgc ctcgccgagc
    34021 aaggctttat taatgatcat gagttcgctc gctattggag tgaatcacgg cagagaacta
    34081 agaaggtctc aaagcgaata attagcggag agctgcgttc aaaaggaatc tcgccagaga
    34141 taattgagtg gatcacaagc gatatcagcg atgatgaaga gtttgccaat gcgatgactt
    34201 ttgccgaacg taaggcaaga gcagttacta gccttgcacc tgaggtggca taccgcagat
    34261 tacatggcgc cttatcaagg cgaggatttt ctggccatgt tgtctctcga gtattggcag
    34321 agctggggat ttcgcgctca taaattagct ctagagatag ataggtacga ttcgctggtg
    34381 agccgtacct atgtaattca aactctgggc tgccagatga acgttcatga ctcagaacgt
    34441 attgcaggct cactcgatgc tgccggatat gtccctgcgg cagatggatc tactcctgat
    34501 ctaatcgtct ttaatacctg cgcagttaga gaaaatgctg ataataagtt atatggccac
    34561 cttagtttcc tggcgccgga aaagaagtta agaccagagt tgcagatagc aatcggtggc
    34621 tgtcttgctc aaaaagataa gtcagtgatt ttggagaaag ctccctatgt tgatgtcgtt
    34681 tttggcaccc ataatatggg ctcacttccc gctctccttg agagagcaag ggttgaaaaa
    34741 agagcgcaga ttgaaattaa agaatcgcta gagcactttc cctcgactct tccagctcgt
    34801 cgccactcac cattttcagc ctgggtctca atttcagttg gatgtaataa cacctgcacc
    34861 ttctgcatcg ttccagcgct tcgaggcaga gagaaagatc gaagtagcgc tgagatttta
    34921 agtgaaatca gagctctcgt tgcagatgga gttattgaga taactcttct aggccagaat
    34981 gtaaatgcct atggtgtgga ctttggcgat cggggagcct ttgcaaagtt gctaagagcc
    35041 tgcggcgaaa ttgagggtct ggagcgagtg cgctttatgt ctcctcatcc aagagatttc
    35101 accgatgatg taatccaagc aatggctgag acaaaaaatg tgatgcccca tcttcatatg
    35161 ccactgcaat ctggatctga tgcaatcttg gcagcgatgc gccgctccta tcgagttgat
    35221 cgctatcgct caatcattag caaagttcga agcgctcttc ctaacgcaac tattacgact
    35281 gatgtcattg tgggatttcc aggtgagagc gaggcagact ttcaagcaac acttgattta
    35341 tgtagcgagg ttggctttct tgctgcatat agctttaagt attcaaagcg cccaggcacc
    35401 ccagctgctg ttatggataa tcagattcca gaacaaatcg ttgcccagcg ctatgaccgc
    35461 ctccaccagc agataaatca aattgcagct cgggtaaata atgaagtact tggcaaaaga
    35521 gttgaagttt taatcactga tattgatggc tctagcgcaa ctggaaagag tagagatttt
    35581 cgtctcgttc actttgaagc tccagcaact gctcggccag gagatattgc tgagttaact
    35641 attaaagaga gcaaggctca cttcatcctt ggagatagcc agagtgtaag cattcgccca
    35701 actcgcggcg gagacgcttt tagcgccaga aaggctgagg cagaaacaaa aggagtagcg
    35761 ctaggaatgc cgactctaaa gagcgttcac tctaaatgag cgtcattgtt atctgcggag
    35821 caactgccac tggaaaaagt gatttagctt tatcacttgc tgaggctatt gggggcgaga
    35881 tagtaaatgc cgactcaatg cagctctata aaggcatgga tattggaact gcaaagctgc
    35941 cacttagtaa gcggcgagga atcccgcatc acttacttga tgttttaagg gttaatcaag
    36001 aggcatctgt tgctcagtat caaatcgatg ctagaaatat aattgatcag ctcttagagc
    36061 taaataaacc agcaattgtt gttgggggaa ctgggctcta tatcaaagca atattagatg
    36121 atcttaattt tccagatact gatccagcgc ttagagagaa gatagctaaa caaggacagg
    36181 agttgggtca ggatgttatg catcaaaggc ttgctaagtt agatcccgca gcagcagctg
    36241 ccattcctaa ggagaattta agaagagttg ttagagcact tgaagtaatt gaattaactg
    36301 gcaagcccta caccgcaaat ctgccaaggg ctggaagtag taaataccca catgcaaaac
    36361 aatttggact tgttatggag cgctcaactc ttgcatctcg catcgatgct cgagttgaaa
    36421 agatgtggga tgaagggcta gtagatgagg ttaaaggcct tatttctcaa gggttattgg
    36481 aggcaagaac agctcaagcc gcccttggtt atgcccaggt aattaagttt gccaaaggcg
    36541 agttaactca gagcgaagcg aaagaggaaa caaaacgggc aactaggcaa tatgccagaa
    36601 ggcaggagac ctggttttca agagatgagc gaattacttg gattaagggt ggttcaaggc
    36661 agcaaatgct tgaagaaatc atctcctcta ctattagccc tagatgaaag ctcctcttac
    36721 cgccacttat ggccatggca ctgagaatga ctttgtaatc ctctttgatc cagatgataa
    36781 aaataatctc tcttcaaaac agactgcagc gatctgcaat agggcaagcg gcattggcgc
    36841 agatgggcta attcgaataa taaagcgcga tggtaaatgg tttatggatt atcggaatgc
    36901 agatggctcc cttgctgaga tgtgtggcaa tggcatcaga gttatggctc ggtatttagt
    36961 agatagaggc catcaaggcg ctgggatatt ttcaatacta actcgtgatg gggctaagta
    37021 tttaagcgct gatgtggcag gagatatcag tgtaaatatg gggcaggttg aagtaatcga
    37081 tggcgaaatt acagctgcca ataatggcag agtctggagt ggttataact taaatatcgg
    37141 aaatccccat gccgtggtct ttgttgattc gctagatgat gtcggtgatt taaaggatcc
    37201 gccagtagtt cgcccgaaag aggagtatcc agagggcgtt aacgttgaat ttgttcaatt
    37261 tcttgaaaat ggcgaactcg caatgagagt tcatgagcgg ggaagtggtg agaccagatc
    37321 ttgcggaact ggaacctgcg cagttgctct agctgcaact ttaaagaagg ggatgaaatt
    37381 gccagctaaa tgggtgatta atccaccagg tggaagattg gttgtagaaa ttgatccaca
    37441 ctccaatgcc acgctaactg gccctgcagt actactaaaa gactttgatc tggagcccta
    37501 tcttggctaa gaaaaaagag ctgccagaga tagaggattt aatctctgag agccagcagg
    37561 tggcagcaga gtttgattct gagattttta ttcctgattc atatcaatca acgcagcagg
    37621 atttaagtga tagacaggcg ctgcgaagag ttattggcct ctcaactgag ctaagtgata
    37681 tctctgaggc cgaatatcgc cagttaagac ttgagcgagt aattcttgtt ggagtctgga
    37741 ccgagggaag tgttaaagat gcagagaact ctatggagga gcttgcagcg ctagcagaga
    37801 cagccggatc aacagtattg gatgccctta ttcaacgccg agataaacca gatccagcaa
    37861 cctttatcgg ctctggaaaa gttagcgagt taaaggcaat tgttaaatca acaggagccg
    37921 ataccgttgt ctgcgatggc gaattaagcc cctctcaact aagaaattta gaagataagg
    37981 tgaaagtcaa agtagttgat cgaactgcgc tgatattgga tattttcgcg caacatgcca
    38041 aaagcaaaga aggtaaggcc caagttgagt tagctcaaat gagttatatg ttgcccagac
    38101 ttcgcggttg gggtgattca ctatctcgcc aagcaggtgg aattggtggt cgcggacctg
    38161 gtgaaacaaa gatagaggtt gatcgccgca gaatccgcga caagatgtca aagcttcgca
    38221 gggagatagg cgatatgaaa gtcgcccggg ataccaagag acaagagcgt aagagaaatt
    38281 caattccctc tgtggcaatt gcgggatata ccaatgctgg caaatcatcg ctattaaatc
    38341 gtttgactaa tgctggagtt ttagtgcaga acgctctctt tgcaactctt gatccaactg
    38401 ttagacgctc agagacaagt gatggtcgca cctacacgct aactgacact gtgggatttg
    38461 ttaaacacct gccccatgac ttaattgatg ccttcaaatc aacgctagag gaggtctcag
    38521 gagctgatgt aatcgttcat gtagttgatg gctcacatcc agatcctcta ggtcagataa
    38581 aggcagttag aggggtaata gctgatattg ggggagcaaa aattccggaa attatcgcgt
    38641 taaataaggc agatatcgca gatccagaag tgattgctca ggtattgatc caagaacctg
    38701 atgcttatct aatctcagtt catagcggag ttggcattga aaaactcatc cgagctattg
    38761 agtcatctct tccaaggcca aggattgagg ttagaactgt acttccatat gacagagggg
    38821 atttagttag tcagatccat gaacatggcg agatattaag tcaagagtat ttacctgaag
    38881 gaacatcgct tcatgccatg gttgatggat ctttagcgca cgctcttgag aagtactccg
    38941 ttaattaata tttagagcga gcgaagaaca gctgttacca caccaagaat ctctgcttga
    39001 tttccatcaa taggttggta gctatcatta gctggcagta accagatttg accgttcttt
    39061 ttacttaaag tcttaacagt tgcctcgcca tctatcatcg ctgcaacaat ttcaccttta
    39121 ttagcatctt tctgagagcg aataactaca taatctccat cgcagatggc agcattaatc
    39181 attgaatctc caacaacctt gagcatgaag agatcacctt cgccaacaag tgatgcagga
    39241 agaggaaaga gctcttcaac attttgctct gcaagaattg ggccaccagc tgcaattcga
    39301 ccaactaatg gaacgttatg agttctctct ggcttaatgt cttcaaatct ttcatcacct
    39361 ggggtgagaa cttctagagc tcttccacga gatgggtcgc gacggatcca accttttgat
    39421 tcaagtgcct ctaattgata tttaacactt gatggggagg agagccctgc ggcttcaccg
    39481 atctcacgca ttgaaggggg atagccatta gtctccatcg cctcttttat tgctagcagg
    39541 atcttggtct gccttggggt gaggccattt tcatcaacgg ggccatcagg cagttcagtc
    39601 agctttgaag ggtttttgct catggattga gtttagaaca aagttgcgaa gaaatcaaac
    39661 aaatgttcta aaaaaacttg ccgatgtcgg tgggtaggtg tacctttgct cttagaacag
    39721 acgttcgaag agtatcccca ctctctgatt agctcggttc tagaagtatc tggagagaga
    39781 agatgacgac gatcgccatc aatccgttcc ttgctaaaga tgccaggcgc gtgaaaaccc
    39841 ctataaatca aggcccagca gctctgcgac taaccccccg tggtcgaggg ctagcaaggt
    39901 tggcagtcat ctcttcactc tccatactgc ttctctctgg ctttgccgcc tttaatggcg
    39961 caacggcagg atctaatagc gagtcaatct caagtcctta tgtgaagatc agcgtgaagc
    40021 caggagatac cctctggtcg attgctgagg ggatcgcgcc gcaggctgat agacgagctt
    40081 tggttgcaga tattgttgaa gtcaataact tgaaatcgcc aaacctccag gccggccaga
    40141 agatttatat cccgacacgc ccgtagaggc tcaaacatct tttaagttgc ggaactctca
    40201 accgttgctt tactcttacc cctagatatt ggggttgttt taggatatgc ttccataagt
    40261 agtggttagt acctaaggct tcaatacacc cgatttcagg aaatacccag gattgagagg
    40321 acgacacgcc gtgaattgcc ccttctgtcg ttttgaggat tctcgagtca ttgactcgcg
    40381 tccaagtgat gaaggcagct ccattcgtcg caggagagag tgcacccaat gcggttcacg
    40441 tttttcaacc tctgaaactg cagttctctc gattatcaaa cgcagtggag caacagagcc
    40501 ttttagccgc gagaaagtta tcaacggtgt tcgtaaggcc tgccagaacc gcccagttga
    40561 tgatgatgat ctagcgctac ttgcaatgag ggttgaagag gcgctgcgct ccacaggaca
    40621 ggcagagatc gaagctcaag aggttggcat ggctatcttg ccgccacttc gcgaattaga
    40681 tgaggtcgcc tacctgcgtt atgcctcggt ataccgtaac ttctcctccc ttgaagattt
    40741 cgaaggcgag atcgctcttc tccgggccct gccgatcgaa aagtcagaaa tagagagcga
    40801 atctcaagtt gcctccccgc aacttaaatg aaaagtccga taagtagata gcagagaaaa
    40861 aattaaataa aaaaagatag aaaagtttta aaaacaaaat taaaaattca agaagaaact
    40921 ggagaaatta gaaatgtcgg acacagtaat caatcgccca tcggggaaag ccaagtcaga
    40981 aaaaggctta agtatggagc ggatttacac cacagctggt caacacccgt atgaaggtgt
    41041 taattgggag agacgcgatg tggtgcagac caactggaag agcggcgaag ttgtctttga
    41101 acaacgcggc gttgagtatc cagatttctg gtcagttaat gcctcaacca ttgttacaac
    41161 aaagtatttc cgcggcgcag tgggagctga aaatagagag cactcattaa agcaagttat
    41221 cgatcgcgtt gttcttacct acaccaaagc tggaaaagaa catggttatt ttgcaactcc
    41281 agaagatgct gaaatttttg aacatgaatt aacttacatg ctcctgcacc aggtattttc
    41341 ctttaactct ccagtctggt ttaacgtagg aacatcagct ccgcagcaag tatctgcctg
    41401 cttcatcctc tcagttgatg acacgatgga atcgatcctt aactggtatc gcgaagaggg
    41461 catgatcttc aaaggtggat caggagctgg attaaatctc tcacggattc gctcctcaaa
    41521 agagctcttg aaatcaggtg gcacagcatc tggcccagtt tcatttatgc gcggggctga
    41581 ttcctctgct ggaacaatca aatcaggtgg cgcaactaga agagctgcca agatggttgt
    41641 tctagatatc gatcacccag atattgaaga atttattgaa actaaggtaa gagaagaaga
    41701 caagattcgc gcccttcgcg atgctggctt tgatatggat cttggtggca aagacatcat
    41761 ctcggtgcaa tatcaaaatg ccaataactc agttcgcgta aatgatgaat ttatgcgcgc
    41821 ctatgaaagc ggagccaagt ttggtctaac agctcgcgca acaggtgagg taattgaaga
    41881 ggttgattct cgtcaactat tccgcaagat ggcagaggct gcctgggctt gcgcagatcc
    41941 tggaattcaa tatgacacca caatcaatga ttggcatacc agccctgaga ccggtcgcat
    42001 caatgcatcc aatccttgct cggaatatat gtcacttgat aactcctcat gcaaccttgc
    42061 ttctttaaat cttatgaagt ttttaaagag cgatggatca tttgatgcaa agaacttcgt
    42121 taaagcagtt gagatgatca ttactgcaat ggatatctca atttgttttg cagatttccc
    42181 aaccgaggca atcggcgtaa caacaagggc ctatcgccaa ctaggaattg gttatgcaaa
    42241 ccttggcgcg cttcttatgg catcaggact tgcttatgat tcagagggcg gaagagccct
    42301 agctggagcg atcacctcac ttatgactgg aacttcatat cgcagatctg ctgaactagc
    42361 tggaatcgtt ggcccatatg agggatatgc cagaaatgca gctcctcata ctcgagtcat
    42421 gaagaagcac caggccgcat cactttctgc taaatcagtt tcaactcttg atagagatgt
    42481 tttagctgag gctaataagc aatgggatag ctgtattgca cttggcgaga agaatggctg
    42541 gagaaatgct caagcatccg ttcttgcacc tactggaact atcggcttga tgatggattg
    42601 cgataccact ggtattgagc cagatttctc actcgttaag tttaagaaga tggttggtgg
    42661 cggatccctc cagatcgtta accagaccgt cccacaagct ctaaagaagt tgggctatac
    42721 cgatgagacc attgaagcga ttgtggagta catcgctgct aatggccatg tagttgatgc
    42781 tccagggcta aaggttgagc attacgatgt ctttgattgc gcgcttggca caagatctat
    42841 cgcgccaatg ggccatgtga gaatgatggc tgcctgccaa ccattcctct caggggctat
    42901 ctcaaagacg gtaaatcttc ctgctgatgc aagcgttgaa gatgttgaag aggtttatta
    42961 cgaaggatgg aagttagggc ttaaggctct tgctgtttac cgcgatagtt gcaaagttgg
    43021 tcagccactc tctgatgcaa aatcaaagaa caatggcaaa gatgatcagg taagcgcagc
    43081 tcctgctcct gcaactcgta agcgccttcc aaagtcacgt ccttcaagaa caacttcgtt
    43141 ctcagttggc ggggcagagg gttatatgac tgctggtacc tatgaagatg gccagctagg
    43201 tgagatcttc ttaaagcttg gcaagcaggg ttcaactctt gcaggtgtta tggatgtctt
    43261 ctcaattgca gtatcaattg gtctgcaata tggagtgccg ctagagacct atgtttctaa
    43321 gttcacaaac tctcgctttg agccagcagg aataactgat gatccagata ttcgtatggc
    43381 ccaatcaatg atggattaca tcttccgccg ccttgctctt gattacctgc catatgaaga
    43441 gcgcgcagca tttggtatct ataccgcagc agagcgagca gcagcccttg aaaccggtga
    43501 atatgctcaa attgttgagg aaaagataga ggtagttgag gagaagaagg ccagcgcgcc
    43561 agttgcaaag cctgcaaagg ctgaaacttc cctccaccaa attggttctt caacagagct
    43621 gctagagaag atcgctggcg ttaaatcaga tgctccactc tgtgttacct gtggcgtaaa
    43681 gatgagagct tcaggagcct gctatgtctg tgaaggttgt ggcaatactt caggatgtag
    43741 ctagaaaagt aattacagga gggctcatct ctccttgatc tctaacccct cgaagaaatt
    43801 tcggggggtt aaatcattta acgctattta tctagcaatt aagtaagcag tttctaatca
    43861 ttgaatgtga aatcagaata aggttgccac tatgagcgca gagtcagtta gaaaggcgct
    43921 agcagcagca gttgaggcaa ttggcggaag ccagagagct ggccagattg caatggcaga
    43981 ggcagtggca aacgctcttg aagatcgcca tcacctcctt gttcaagccg ggactggaac
    44041 tggaaaatca cttgcttatt tagtgccagc tctagttcat ggcaaacgag tccttgttgc
    44101 aacagcaact cttgcgctgc agcgacaatt aattgagcga gatcttccta aggttcaaaa
    44161 ggcgttagag aaagaattgg gaagaagcat cacctttgct gtttataaag gggttggaaa
    44221 ttatctctgc ctgcagaaga tgaatgctga tgactctggc gttgatagcc aggtcttagt
    44281 tgagatttca tcacttgaat cagatgctcg ccgccttcgc cagtgggcga gtagagatgg
    44341 aatctcgggg gatcgagatg atgcccctga ggttgataga cgagtctggc tagccaattc
    44401 aacatctgga agagaatgtg tgggagctga tgaatgtcgc tttggagcgc aatgttttgc
    44461 agtgaaggca aaaactaaag cccagagcgc agatattgtc gttactaatc acaccttact
    44521 tgcaattgaa atcgttgact ctcatccgat attgccagag cgagatgcag tgattttaga
    44581 tgaggctcat gagtttatgg atcgcaccac tcaagcagta actgaggagc taactgcagc
    44641 aagagctagt agagcagcaa atatgctgcg aaaacatctt cccggaaaag ctgctgaagc
    44701 gctgggcaaa gcttgtgatg gttttagcag agcacttgat gagtatggcc aagagtttcg
    44761 cacaaaaagt ggcgatgagc gaaagcttca agaactaccg ttgcttcttg ccccttgggt
    44821 gagaaagatt aaagaggcag cagagagcgc tattgcgcta attaatgccg attcactaat
    44881 tgttgatcca gatgctttag ctgagcgggc tcgagtaaag ggagcggtca atgaaattaa
    44941 aaccaccgca tcaaagatgc ttaagattgg cagtaatcaa gtgctctggt ttgaaccaac
    45001 attttccact ctctatcttg ctccactaga agtctcacat ctattacgcg agaacttatt
    45061 tactaaaact cccgttatcg caacaagtgc aaccttgagc gttggaaata gttttgctgc
    45121 aattgctaag agctttggta ttgatccact agaggctagc caagatgaaa gtagcgaaag
    45181 cgggggagat attgatcctg aaaatctagt atcacttgat gttggttcac cctttgattt
    45241 tgccagccaa ggagcgctct atcttccaag agatctacct gagccaacaa gagatgggcc
    45301 aagtccgcag gctctggttg agctaggtga gttaattgat gctgccgggg gaagaactct
    45361 tgcgctattt tcatcatggc gcggtgttga gatggccgat gaacatctaa ggcgagtttt
    45421 agcagagctt ccgataaaga tcattactca aagacggggc gatagcgttg gagtcttagt
    45481 tgaaaaattt gccagtgatc ccacctcaat tttgcttgga acaatgagcc tttggcaggg
    45541 agttgatgtg ccggggccat cttgtatttt ggtggcaatt gatcgaatac cttttccaag
    45601 accagatgat ccagtgatgt cggcaagagc tgcccagata gatgcatctg gcggttcagg
    45661 ttttatgcaa gtctcactcc caagggccgc tctactactt gcgcagggaa caggaaggct
    45721 aattcgatcc attgatgatc gcggggtagt ggcaattctt gattcaagaa tcgttaataa
    45781 acgttatgga tcagtgctat taaactctat gccgccgctc tggagaagta gcgatggagc
    45841 tgttatcaag gaatctctta aaagactttc tagccaatac aactccacag ggcagtgata
    45901 tccccaaagt cttagttttt ccaatttaga gatgagatca agaaggcatc atctgctaga
    45961 tgcggataac tcttgttgca atgttaatta tcttttcatc tatgaatgct acctatggcg
    46021 cttgtgaagg tgagacatgt attgatgtaa gagctaatca agagagcaat gaagttgtaa
    46081 tcacagttaa gaagggtaag gcgggcagtc aggtatcaac tgcgccaaag ccaagaccaa
    46141 cctcaactac aaagcggact tggattccct ggcttccaaa gccacctgct gcaagtaagc
    46201 cagttgcgct aggtccaaag ccttcaccaa aaccaagagt gaagagaatt tctggctctc
    46261 aaattagcga tcaagttaag agtttgattc caaggggagc gataatcacc caaccgctag
    46321 ataacatctt ggtaaatcag agcgttaatt tcttaactaa tactcccatg cacttcacta
    46381 ccgtccttgt agttcttggc acgcctataa cgattcactt aactccggag tttttatggg
    46441 agtttggaga tggcaatagc cagagcacca agcttgcagg agccccatac ccattgatgc
    46501 ttattgaaaa tacctataag agctcaggcc agaagagagt tgaattaaca acaacttgga
    46561 gcggtttctg gcgagcaggg tctataggtg cgccaattga tggagcaatt gtgcagcggg
    46621 tcagtcggca aatagatgtt aggccagcca gaggtatcta caggggttag ttatcaacat
    46681 ttctgcggct tgaaaaaaac ttccatttct ctatctcaag atgctttgcc aatgacgaca
    46741 cttacaacac ctaaagacct tcttgcagct gttccctttt taattggatt taatccagag
    46801 aattcaatag ttctattgag cctaaaaaat gatgcaatag ctatggcgat acgcatagat
    46861 tttccagatc aaatcggcgc agatgaacta aaacttctaa actcccattt atctcgagat
    46921 agcgctgaag ctattattgc aatcttttat tgcccagaaa ttgggcagag ccagaatgag
    46981 ataatttcag agattctcgc tgctatcact gaatatggac taaatcttcg agaggtcata
    47041 acagttgaga aggatcggta tcgctcatta atttgtgaag atttgagctg ttgccccagt
    47101 gagggaaacc tacttccaga actcattgac tcacgaattg ctgcagagca agttgctcaa
    47161 ggcagaccaa ttcctttcgc aactcttgaa ctgctaatag attcaatttc gaagcttgac
    47221 accgatcatg aattactaga gttaataaga agtatcgagc ctattgatta tgaaaaagat
    47281 ccaatatctt ttcaaaggca aggcgctagc tcagttaatc agtttatgga tgagtttaaa
    47341 tctcatggtc tagtaaaaga caaagcgttg atagcacttc tattggttcg cctagcagat
    47401 ttgcaggtta gagattacgc ccttggaagt gttagcactg agagccttga tctctacttc
    47461 actgcctgga gatggttgct tcgacttgct ccagagggct atatcgctcc agttgctaat
    47521 ttgtttgcag cagtggctta tgagcgaggt gatggagctc ttgcacagag agcgctagat
    47581 agggcagata gcgatgatcc aagttatgca atgagtaaat tacttagaca ggtcttcaac
    47641 tctggatggg cccctaactc atttgcccaa atgagaagtg agctacaccc caaaatatgt
    47701 agtgagcttt tctcgggtac tatctagagg tgaacatcgg cgttcccaaa gagataaaga
    47761 accatgagta tcgagtctca ctaaccccgg caggagctag agaatttatc cgccaggggc
    47821 ataaagtttt agtagaaagt tcggctggtc ttggctgcgc aatctcagat gatgactata
    47881 gaagcgttgg ggcaacaatt attgcctcag cagatcagct ctgggctgag gccgaattag
    47941 tcttaaaagt aaaagagccg attgctgctg aatatcaccg aatgagagct ggtcaagttc
    48001 tatttaccta tcttcatctt gctgcttcaa aggattgcac tgatgcgcta atgaaaagcg
    48061 gtatcactgc aattgcctat gaaactgttg agctaaattc caatcttcct cttcttgctc
    48121 caatgagcga ggttgctggg cgtatggctc cacaagttgg cgcagcagcg ctgcaggcaa
    48181 gtgcaggtgg gcgcggtgta ttgcttggcg gagttcctgg agtaagacct ggaaaggttt
    48241 taattcttgg cggaggaact gcaggtcttg ctgcagcaac tattgccctt ggtatgagag
    48301 ctgatgtaac ggtagttgat ttaaatcaga gcagacttgc ctatattgat cagatattta
    48361 atggagcagt aaagactttg gtctctaata cccatgagat tgagcaagaa gcacttcttg
    48421 cagatttaat tattggcgct gttttagttc acggcgcaaa ggctccgaag ttgattagta
    48481 atgagttagt ttcgcattta aagaagggtt cagtcttagt tgatattgca attgatcaag
    48541 gtggctgttt tgaggattca aaggccacaa cccatgcaga tccaacatat aaagttcatg
    48601 agagcatctt ttattgcgtg gccaatatgc caggagctct gcccctgacc tccacctacg
    48661 ccctttcaaa tgccaccctg ccttacgcgc tagcccttgc caataagggc tggatgaagg
    48721 cgctatctga aaactctggc cttgcgaagg ggctaaatgt ccataatggc cagatcactt
    48781 ataaagcagt agctcaggct catggcatgg caagtgttga gatggacttt tcactctaaa
    48841 ttttctaaag tccgccttag gtcaagagtt ccagcattaa gccccggcta actggacggc
    48901 aaccctccac cgcggtgggg tgctccgggt gaggacctgg tcgattgcaa agtgcatcga
    48961 caagcgcggg tctgacaatt attggcaggc cttaagctcc taatttgcga aaggcaagga
    49021 aaaagtgaaa aaaatcttta aattcatccc attacttcta actgctctag taatcacagc
    49081 atgttcctca tcaagtgatg aaggcgcaac tggtgattca acaacatgtt ctccagaatc
    49141 tcttgcaaca gttgaagcag gagttctaac aattgcaact ggtgagcctg cctactatcc
    49201 atgggtcatc gatgatgctc cagagtcagg tgaaggtttt gaagccgctg ttgcatatgc
    49261 agttgcaagt gagcttggct atgacaagtc agcagtgaag tgggttcgca ccagctttga
    49321 tgctgcaatt gcgccagggc caaagacctt tgacttcaac cttcagcaat attccataac
    49381 agatgagcgc aagcaggttg ttgatttctc atctgcatac tacaaatcaa atcaagcaat
    49441 tgtctccttc aagggaagca agattgatgg cgtcactacc ttgcagggtc ttgcagatgc
    49501 cggagctaaa ttaggcgccg ctgtttcaac tacctcactt gatgtgattg aaggccaact
    49561 agggctaaag gctgcggtat ttaacgataa cgctgctgct gttactgccc ttaagaataa
    49621 gcagattgat ggtctagttg tagatcttcc aactgctttc tatctctcag ctgttgaagt
    49681 tccaaatgga ataatcgttg ggcaaattga tggcagtgat tcaggagatc aaggatttgg
    49741 tctcttgcta tctaaagaca atccgatcac atcatgtgtt tcaggagcag tcgatgctat
    49801 taacaataat ggaacacttc aggccattat tgaaaaatgg ttaacatctt ctgcaggagc
    49861 gccagtactt ggatagttgc cagatacggc aaactactaa gcatggctaa tggctaagaa
    49921 atcagggcgc agttctaata gagctgcgcc ctcttcttct tataactttg gtggcagctg
    49981 gagcccaagt gaaatagaac ttgcaagacg tgcggcacgg cgagcacgca ataaaagaag
    50041 tgcactaatt gcagcactct caagcttctt agttcttgga acccttgggt tagttctagt
    50101 tacctcaccg ggctgggaga cacttagaaa tacattcttt aagtgggctt atggatttga
    50161 aattctgccc aaaatacttc tcgggtttac taccaatgcc acactaactg ttattgcagg
    50221 aacatctgta gcgctcattg gtttagcgat agcgctcctt aggacttcta gatcgccagc
    50281 attaacccca tttaggattc ttgccacagt ttatgttgat gtatttcgtg gtattccaat
    50341 gcttctagta attcttcttg tgggatttgg aattccagcg ctacagataa agggcttaac
    50401 caataatgtt ttggttttag gaactcttgc agtaatcatc acctattcgg catatgttgc
    50461 tgaagtgatt cgctctggaa ttcttaccgt tcacccaagc cagagagctg ctgctagatc
    50521 actgggttta agtaattttc agacgcttcg ccacgttgtt ctgcctcaag cactcaagag
    50581 agtcacccca ccactactaa atgatttagt agcgctaatt aaagatactg gtctagtttc
    50641 aattttggga gtgaccgatg cagtaagagc tgcgcagatt caaactgcaa agacttttaa
    50701 ttacacccca tatgtaatgg ccgcaatcat cttccttctg gtgacaattc ccttgactcg
    50761 ctggactgat cggatgctta gaaactccta tgagcgagag aatgcagtgg ggcaagcatg
    50821 agtcaggcgg tgttgcagct cgagaaagtt agaaagagct ttggaagtga agtagttctt
    50881 gaagatattt cctttaaagt cttagagcat aaagccacag tatttattgg tgcatctggc
    50941 agtggcaagt cgacgctgct gcgctgcata aatttacttg accagataga tgatgggcag
    51001 atactccttg atgggcaaga tatctctgca gcagatgtaa atcctgatga ggtgagaaag
    51061 aagttaggaa tggtttttca atcctttaat ctctttcctc acatgacggt gcttgaaaat
    51121 gttattttgg ctccaacaaa ggttcagaag gtggcaaaag agaaggcgat agatgaggct
    51181 ttggagctat taaataagtt tggtttgagg gagaaggctg atcaatatcc agatcggcta
    51241 agcgggggtc agcagcagag agttgccatt atccgctccc ttgccaggca ccctcgactg
    51301 ctattacttg atgagataac tagcgccctt gatccggtct tggttaatga agttttgagc
    51361 acggttagag atctaaagag tgagggaatg acgatggtgc tggccaccca tgagatgggt
    51421 tttgccaaac aggtggccga tgagctctgt tatctgcatc agggaagaat tctggagtgg
    51481 ggaagtccaa agaagatttt agaaaaccct aaaaagcttg agacaaagga gttcttgcgc
    51541 cgcctccatc aagcaggcag gctctaggcg ggctttcttg ctgatcccaa gccagatgtt
    51601 ggggcaaaac caggaataaa gtggtttgga tgtgcattta cccctgtgga caatttataa
    51661 tatagccata gccaattccc caaaaggcta gcggtaaaaa agaagttttg acagttcgtc
    51721 aatttaaagc aaaggactaa ttcgtggctg tatctcgtgc gcccaaaaac gcaggcaaga
    51781 agaagagcgt tgcaaaaaag agcacgaaga gcgttgctaa aagttcaata aagaaagata
    51841 agaagggcgc taagtccaaa ggcccaagac tatttcctac caaggcagaa cttgaagcca
    51901 tgaagctggc aagagaagca aaagctaagc cggctgaaaa aagtggcccg cgacgctttc
    51961 caacaaaggc agaacttgaa gcagcaaagg ctgcagctgc tggagtaagt aagcctgctg
    52021 gcaagaagat aattgaaaaa gttgatggcg aagaggttga actagaagaa gttgaattag
    52081 aagacctaga aaagttagtt gctgaaactg gggaagacgt tgttgaaggc gcaagtgatg
    52141 ttcgagttgt taatctaact gaggaagcca agaaagaaga gggtgagttc acccttaagg
    52201 attctgaaga agatgacgca cctcctcaga ctgtcttaac agcaggtgct actgcagatc
    52261 cagtaaaaga ctatctcaag cagattggtc gagtagctct actaaatgct gaacttgaag
    52321 ttgaacttgc tatgagaatt gaagctggat tatttgctga agcaaagttg aattcaggcg
    52381 agaagatgga acgttcattt aagcgagagc tggagtggat tgcagaggat ggtaagagag
    52441 ctaagaatca tcttcttgaa gcaaaccttc gcctcgttgt ttctcttgct aagcgctata
    52501 ccggtcgagg aatgctattt cttgatctaa ttcaagaggg aaacctaggt ttaattcgcg
    52561 ccgtagagaa gtttgattac acaaagggat ataagttctc aacttatgca acctggtgga
    52621 ttcgccaggc aatcacacgc gctatggcag atcaggcaag aactattcgt attccagtac
    52681 atatggttga agttattaat aaattagcgc gagttcagcg ccaaatgctt caagatctcg
    52741 gccgcgaacc aaccccagaa gagctggcta aagagcttga tatgacccct gagaaggttg
    52801 ttgaagttca aaaatatggt cgcgaaccga tctcgcttca tactccactt ggcgaagaag
    52861 gagattcaga gtttggtgat ttgattgaag actctgaggc gattgttcct gcagatgcag
    52921 tttcatttac tctccttcaa gagcagctcc actcagtgct agatactctc tcagagcgtg
    52981 aggccggcgt tgttgcgatg cgctttggat taaccgatgg ccagcctaag acccttgatg
    53041 aaattggcaa ggtatacggc gtgacccgcg agcgtatccg ccagattgaa tctaaaacta
    53101 tgtccaagct tcgccatcca tcaagatcgc aagtactacg cgattaccta gattaggaga
    53161 agagcaatga gtgagaaggt aaagatattc gttaagccta atggctctat tcgcgtcaca
    53221 ggtgaagttg attttgtcga tgcgcaagga aatgtgattg agagtaaaga gaatttctct
    53281 ctctgtcgct gcggacactc taaagagaaa ccattctgtg atggctccca tcgagatgca
    53341 ggatttaccg ccgaataact cgctctgggc agaaggtttt aagcagagcg gtaagagaaa
    53401 agaatttaat aattcttacg atgtcgcaat aatcggtggt ggattctcag ggctctggtc
    53461 ggcctatcat cttctgaagt taaatcctaa actctctatc gttatttttg aggcaaaatc
    53521 aattggctat ggagcaagtg gaagaaatgg tggctgggtt tcatcagatt atccggtcta
    53581 tcgaaaaaca cttgaaaagc gccatggcag tgatgccact aacaatcttt tcaaagagct
    53641 tgcaaatgcc attaatgaga tcggccagat agcaggacaa attgcaccca aggcaagttt
    53701 tgttaagtca ggaacgctca catttgctag aaataaggcg caggagagac gattaaaaga
    53761 gagcgctgat tcagaacatt tctggaagtc tgcaggtgag cttgatcagt taattcaagt
    53821 atctgattta aggggcggat tatttaatcc gcaatgcgca acgattaatc cattccaact
    53881 tgttttgggg ctcgccaaat acctggagag caaaggagta gtaatagccg agggggtatt
    53941 tgctacccaa acaaatggcg gagtcttagc aaatagctca ctagtgaagg ctccggtagt
    54001 gattagagcc agtgaggctt tctctaatcc tgggcgagaa tttatcccgc tctactctct
    54061 catgattgct agtgaagctc tgcctgaaga aatctgggat caaataggag ttaaggatcg
    54121 cttcaccttt gctgaaggga cgcacttggt gaattacgca caacgcagtg cagataatcg
    54181 ccttgcaatt ggtggtcgcg gggctagcta tccatttggc tcaaaactta ttgaagaaaa
    54241 ggaaatgact agctctgttc atcaaagctt agagaaaatg gctaggcgat ggtttccagt
    54301 tttgaaaaat gtgaaattta ctcatgcttg gggcggggca gtggcaatta cccgtaattg
    54361 ggagccatat gttcaatttg ataaatcaac gggatctgct cgccttggtg gctatgcagg
    54421 agatggcgtg actatgagtt atttagcaag caaaatattg gctgatttaa tcgttggcag
    54481 agaaagtaat gtcacaaatc tgcactttgt aaatcgcgaa attagaaaat gggagataga
    54541 accacttaga tatctagcag ttaactcgct agtgaaatta agtgggattg cagatagaga
    54601 ggaagctaga actgggaggc ccagtttggt ctcaaggctt atttcgccgt tgattctgcg
    54661 ctaaggcgct cagtttcatc atcgatgcga gcggctgtgc ttgctagccc atcttggtat
    54721 tttttagcgt ggtgagcaca gaaaagtaat tcaaatccat tggccattac cgcacgaaca
    54781 taagcctgag ctccacagcg atcgcaacga tcaagagcgc taaggggaga tgaggtaagc
    54841 aatagatttt cagtcatttc tggccaacct ttcctgtgct tgttaggttc tagtggaaca
    54901 tcaaaccacc tctgggttat tccccgcagg tcgaattcaa agttttttct cataattgat
    54961 gactttcttc actgaaaatc gaaatcctca cccttttcgg cgcgtattgc cagactcctt
    55021 tgatcccata gatacccttg cccatcgtgg cagagacttc aagcgcagac caagtgcgca
    55081 gtgtagaaaa gtcctactca gcaaaagatc tctcagtatt agaaggtctt gatgcggttc
    55141 ggaagcgccc cggaatgtat atcggttcaa ccgatggtcg cggccttatg cactgtctct
    55201 gggaaattat tgataacgct gtggatgagt ctcttgcagg ttattgcaag aagatagaaa
    55261 ttaatttaga aaaagatgga tctattgaag tccatgatga tggacgaggt atcccagttg
    55321 ataaggaacc taaaacagga cttactggag tagaagttgt gatgacccgc cttcatgctg
    55381 gtggaaagtt tggtggtggc tcatatgcgg caaccggagg ccttcatggc gtaggagcat
    55441 ctgttgtaaa tgctctggca tctcgccttg atgttgaagt tgatcgagat ggaaaaattt
    55501 attggatgtc ttttaagcgc ggcgttgctg gaatatttga tggcgatggc cctaatgctc
    55561 catttgaacc taaatcaggg cttcgcatca ttggaaaagt ctcggcaaag atcactggaa
    55621 cgagaattaa gtggtggtca gataaacaga tatttctaaa ggatgcacag catgatttag
    55681 aggagatcta caccagggct cgacaaactt cttatctagt tccaggtctc actctggttg
    55741 ttaatgacaa tcgcagtaaa gataagagct cggagatttt ctttcataaa ggtggaattt
    55801 cggaatactg ccactttatt cagccggatg aggcggtagg agatgtaatt cgcttaactg
    55861 gccaaggcca ttatcaagag actgttcccg ttcttgatga aaaaggccat cttgagcctc
    55921 aagaagttga gcgagatatg ggcgttgata tcgctattaa atggggcaat ggttatgaga
    55981 gcacagttcg ctcctttgta aacatcatcg caaccccaaa gggcggaacc catgtgcaag
    56041 gctttgaaaa ggctttagta aaaaccttta atgagttcct tcgtgctaaa ggcgttctaa
    56101 aaaagaatga acttgatgtg attaaagaag atatcctcga aggattaacc gcagttgtga
    56161 cagttcgaat gcaagaacca caatttgaag gtcagactaa agaggtttta ggaactcctg
    56221 cggtaacaaa gattgtgact caagtagtct cagatgagct taagaagttc tttacaacca
    56281 ctaaacgaat cgataaggcc aatggacttg tagtgcttga gaaagttgct aacgcctcaa
    56341 gaactcgtat ttcatcaaga gcccacaagg agttgcaacg cagaaagaac gcccttgaga
    56401 gctcatcact tcctgccaag ctctctgatt gtcgttctga ggatgtttcg cgaactgagt
    56461 tatttattgt tgaaggtgag tcagctcttg gaactactaa ggctgcaaga aattcggagt
    56521 tccaagcaat cttgccgctg aaaggaaaga ttctaaatgt tcaaaaggcc tcgctttctc
    56581 agatgcttga taacgcagag tgcgcagcaa tcatccaagt aattggagct ggatctggaa
    56641 agtcttttac gctagaggat gctagatatg gaagagttat tctgatgtca gatgccgatg
    56701 ttgatggcgc acatattcgc tgcttaatgc tcacgctgct ctatcgctat atgaagccac
    56761 taattgatga tggacgagtc tttgctgcaa ttccgccact tcatcgcatt gaagttattg
    56821 gcggtggagg caagaaggga gaggtgcact acacctactc cgatgatgat atgaaaaaac
    56881 tattgaccga cttgaagaaa caaggcaagc gatggaaaga gccactgcag cgctataaag
    56941 gcctaggcga aatggatgct gatcagctcc gcgaaacaac tatggaccct gataagcgca
    57001 cactaagaag aattaccatt aaagatgcgg cagaggctga aaagatcttc gaactattga
    57061 tgggtaatga agttgcgcct cgaaaagaat ttattgcaac tgcagatatc gatcgcgaaa
    57121 gaattgacgc ttaagcaatt agagtgcgtc gcgccgttgc gtatttggcg ctaacttcgc
    57181 ttaactcatc tgatacttga tttcgaagcg cctcttcact ctttaaaagc gcagtcagct
    57241 tggagatcgt tgacttcaaa tctttagcct cagtatctaa ctcaatcttt gacatcttgg
    57301 ttagtcttct tagaggcata tctaggatgt aggtagcttg ttcctcactt aatttaaaac
    57361 tcttgataag tgattctttt gcttcatttg catcatcgct tcctctaatt atttttacca
    57421 ctttatcgat atctataatt gcctttagaa ggccttcaac taggtttaga cgggcagtgg
    57481 ccttagcttt tctaaagttg gatcttcttg ttactacctg gattcgatga tcaataaaga
    57541 cctgaagcag ttctttcaag cctaaagtct ttggcttgcc attaactagg gcaacagcat
    57601 tgatagagaa ggcatcctca agtggggtga gcttgtagag gttttctagt accgcctctg
    57661 gctcaaaacc atttttcacc tcaacgacaa cctttgttcc agattgtcca tcggttagat
    57721 caataatgtc tgaaatgcct tggatcttct ttgatttaac tagctcgcta atacgttcga
    57781 ctaccttttc aggtccgata ttaaatggaa gttcggtaat agttattcca gatttacgag
    57841 cgctcacttt gccaatttca actttagcgc gaactttgaa agcacctttt ccgctctggt
    57901 aagcctcaag aatgccctct ttgccaataa tgcttccacc agttggaaaa tcaggtgcag
    57961 gaatgaattt cattaactgc tttaaggtcg cgctgggatt ttcaattaga tgctttgttg
    58021 cttgaattac ttcacctaag ttatgaggag ccatatttgt ggccattcca acagcaatac
    58081 ctgttgcgcc attaactaag agattaggaa aagctgcagg tagtactaat ggttctaata
    58141 tctgcccatc ataatttgca ccgaaatcaa cggtatcttc atcggcttcc tcaaccatca
    58201 ttagcgcagc acttgcaagt cttgcttctg tgtagcgcat agctgcaggc ccggcatcaa
    58261 gcgaaccaaa gtttccatgt ccatctacta atggcaggcg cattgaaaag ttctgcgcca
    58321 tgcgaactag cgcgtcataa attgctccat caccgtgggg atgcaattta cccatcacct
    58381 caccaacaac acgcgcgctc ttaacatggc ctttgtctgg acgtagcccc atctcagaca
    58441 tctggtggag aattctgcga tgtactggct ttaatccatc tctggcatca ggaagcgctc
    58501 tggagtaaat aactgaatag gcatactcaa gaaatgaatc tgacatctcg gatgagacat
    58561 cgatatcaac aattttcccg gggaattctc ctggcttagc agctactact ttcttgcccg
    58621 tgctcttgcc tgaatctgat ttagccatgg cgtgattctg ccaagtaaag tgtgaattac
    58681 ttgggattga cgctcggtct agccccgata gttgtgacac atccactggc taatgtattt
    58741 ccagccctta gagatgattc taaatcttta gattcaatcc atcttccaat gaatccagca
    58801 gcaaatgcat ctccagctcc ggtggaatca attgcagtaa ttggcagaat tggagaattt
    58861 actatctctt gccctcttga tttaccaatt gccccttggc tacttgtagt gatgacaacg
    58921 gtctcaacat ctttagacag ttcatttaag gcaccttcaa tatcgctatt tccagttaga
    58981 aattctgcct cagcctgatt cataattaac agatccatta atttacatct ctggcgacag
    59041 gcatctaccc caaggttgct catcactcca acagatccgg gattaaagaa gatttctaaa
    59101 ttagcttctc tcattttgtt gatcattcga agaactcccg cttgggattg aggattaaag
    59161 agggcataac ctgagagaaa tacagcgcta aatctggtga gatcgggtag atctaactcg
    59221 ctaagacctg agtttgctcc tctatccgga aacattgatc tctcaccatt ttgatcaact
    59281 agcaccacaa caattccagt tcgatgctct tttaaaaact caggtttata ttcaatgttc
    59341 caggcatcaa attcattagc aataactcga ccagctgcat catctccaag acggcagaca
    59401 taaaagactt tatggccaga tgatgcgagc caggtagcgg tatttgctgc agctccgccg
    59461 ccatgggttg aaatttgaga gattgtgtca ctgcctagat acatctgccc ttgcatctta
    59521 acaattacat caagcatggc atcgccaatg cagagaattt gagccatagc tatctaagag
    59581 ctactgcaat ctgagctgct agagcagcat ttgccttaat tatctcaaca tttactctta
    59641 aggattcacc tttggaattg gagtggaaat attccagaag aaatggcgtc acagcttttc
    59701 cgataattcc agctgtctta gctttagcaa gaccatcaaa aaggatttga tcatgtagag
    59761 ctttatccat ttggtttcca acaggatttg ccacaattag cgctgaatta tttgtggcta
    59821 catcacttcg agccctccaa atttgggcaa tgtcttgcgg cgtatcggct ctatgttcta
    59881 agtcaaagcc agagtctgtt agataaaaac caggaaattt atttgtctta tagccaagaa
    59941 ttggaacaga caaggtttca agtctttcca aagtagcgct gacatccaga atggattttg
    60001 ctcctgagca gacgattaag attggagtat ttgctaaagc tagaagatct gccgactcat
    60061 cccagctctg ccaagcttca cgatgaactc cgcctaaccc tccggtggca aaaagcgaga
    60121 taccagccag attggcaata tgagaagttg cagcaactgt agtagcggcg ctctttttct
    60181 gagttgaaat gattgccaaa tctcttatcg aagcttttaa gatgttttca tcatttgcaa
    60241 tttgcgttaa ttgatctggc tctagtccga tatgaatctg tccatcgata attgcaatag
    60301 ttgctggcgt tgcgccagtt tcagtgacga tttgctcaac ttctagggcc acctcaagat
    60361 tcttaggccg gggcagtcca tgagtgataa ttgtcgattc aagagcaaca actgggctct
    60421 tatctttaag ggctctagct actgcagctg agtatttgat catgagaggt ctaggttact
    60481 ggaaagatgg ggtagtggcc tacctgctct cagatttatc tcaatcgata tttgcaacac
    60541 ttggggtatc taactgcatt aattctttag ggcttagtaa taatgaaggt cagagagagt
    60601 gcttacttct tgtagatggt atgggaatca atgctttaga gatagttggt aaagagctac
    60661 cgatattttc acaaattaaa aaccataacc aattgcaagc tactttccct tcaacaactt
    60721 ctgccagttt gacctctctt ggcactggtt gtgcggtggg cgtccatgga atggttggtt
    60781 acacgatgag agttcccaat agcggaactc cagagcgact attaaacgcg cttaagtggg
    60841 atgagcgagt agatccctac atctggcaat cagaggcaac tttgtttgag cgagcaaaaa
    60901 aacaaggagt taaggtctct catattgcag caaagcgtta tgaagaaaca ggtttcacta
    60961 gagctgcact tcgtggcgct gattactatg gagttaatca ggtcgatgag atggttgatc
    61021 aagcaaagag cgcgcttagt aatactagat cttttgctta tctatattta aatgatgtag
    61081 atgatgcctc gcatcgcgag ggcttaggct cagagaaatt tcactatgcc atggcaaaga
    61141 gcgcagattt aattttgaaa cttattgaga atctacccaa aggaagcagg ttatgggtca
    61201 cctcagatca cgggatgatt aatcgggatg attttgtagt tttaggaaaa gaaaatgacc
    61261 tgcttaaaaa cgtcgagttg ctagggggcg agccgagggt gcgctatctc tatctaaagc
    61321 caggtagcgt tgatgagaca aaatcacaat ggcaggaatt ctttaaagaa aaggtaactc
    61381 tctacactcg ccaagaggcg attgcattga atttattcgg ccctgaagta ttagataaga
    61441 atatggagcg cattggcgac ttaattgcta ttgccaacgg agaattcatt atggttgagg
    61501 gtgaaaggca agagttacaa ctatctatgg ttgggcatca tggaggaatt actcaagctg
    61561 agactgccat acctttatta agtgcagata tttaaggttt tactcttcct cttgttcatc
    61621 tttcttcttg cctcgcccaa acataatgtc atcccaagat ggaattgaaa tccgcttggt
    61681 tactccatcc ttcttggcat caggagcaat ctcttttact ccacttgtct cctctaaaag
    61741 gtttggatca gttctaacag caactaagcg aggcggctga ggagattcac cttccatggg
    61801 aaagagaact ccatggtcac taagtcgatc gtttttgggc gcactctttt gagaaacttc
    61861 ttcaccatta atccatctag cgccatcatc tcgtgagtgc agtgttcgct tatttgcatc
    61921 aaagctccag gttgcctctg attgcccatc tcgtgatgga taggtgagaa ctagatgcca
    61981 acttccatca tctagtctcc aggtattcca agagatctga ttcatattta cgccccgagg
    62041 agcaagacgt tgaataacta actctgagag gggaagagaa gttccttttg gtgaggcctt
    62101 ctctgccaat tcaattatcc aagcgcgctc ttgcaagatc ggacttgcat aacgctcaac
    62161 tttttcaata gaaagtcctg agatgcgagc aacttcagca aagctttcgc cagctctaag
    62221 gcgagcttga atttctttaa tggaaaattg tggagcgctc tcaactacgg ttgctaaacg
    62281 gcgctcatta acagcagcgc gcaaagtgtc actgactctt agcgagaaat tattaccatc
    62341 gctatcactt agctctagat gctctccatc ttgagagcgt gcaataaagc gcaagtcagt
    62401 catgggtaaa gattgccata agattactga aatctgcggg aaattaggct tagagttgaa
    62461 ttacttttgc cttccaaagg ctaagaaatg gtagaagcga aagtaaagca attaacatac
    62521 aggcaactgg caccataaat gcgacagttg agccatatgt gtcaatgatg tatccagtaa
    62581 ttgcagcagg aatagcccca ccaagattaa gtgcagagat cgaccatgcc aaaacttcag
    62641 tggttcgctt gggactaacg gagcgctcag caacagcaaa tccggctgtt agtagcggcg
    62701 ctattgctag gccattgata aatagtgcaa tagccaaaat tgttaagttt ccagtaaata
    62761 tctgtgcagc aaaataaatt ggaagggtca gaagggctag agcaaacact gagagaataa
    62821 atcttcgtgc ctcattaatc ttccaatgca acgacccact tataaatgct gagattccag
    62881 agctaaagga ccagatcgcg atatagaaac cgctatattc acgaatacca aattcatcaa
    62941 gatagccaac aagtaccaag ccagttgatc caaagaatgc tccaactgcc atcataggaa
    63001 taaataaagc ttgaatgaat ctatctttta taagcaatga atgatcatcg ccgacttcgc
    63061 ggggatgggc tggcggctga gttctgcgct gcgagagaaa gagcagcgtt cccacactga
    63121 taaatcccat tgcagtaaaa attgcggcct ctggaaagaa gtaggaagca gtgattgcag
    63181 caagaacagg gccgctagta aagatcactt catcaacaag tgcttcaaaa gaatatgcgg
    63241 tatcaattag ctttcgatca tcaccaatta catgaatcca gcgccttcta accatctgcc
    63301 ctgagccaat cacaaaacct tcaaagatta tcgctgcggt aaaccaactc caggtaggag
    63361 catcgctctt aaccagataa acaaagaggc caaggaaaat tagatgaact ggagctgtta
    63421 tggataaaac tttattttga ccgatttggt ctgcaaccct agaccaaagc ggtgtggcca
    63481 ccgacaagat cagtgatgcc accattgata acgcgccagc tagggcatat gagccagtct
    63541 cggcaacaac gaagagaaca attgcgatgt agagcattga aattggcatg cgcaaaatga
    63601 agcctgcaaa agagaagctc caacctccag gcacggcaaa taatcttttg taggaggtga
    63661 gcatgaaggc aagtctactt ggcatcgaag aaactatgat tagattggaa tatgtcgtta
    63721 aaccttgagt tattagccct tgctgagaaa attgcgctaa gcgctgggga gttgctcttg
    63781 gatagaccaa gtaagtttga attagatgaa aaaagtggtg ctctagattt tgcgactcag
    63841 aaagatcatc agagcgaatc tttgattgtg agccaaattc ttgctcaaag gcctttggat
    63901 ggattgattg gagaagaagg ggcaaataaa gagagtgaat ctggagttac ttgggtaatt
    63961 gatcctattg atggaactgt gaattatcta tatgacttac ctggttggtg catcagtatt
    64021 gcagcaaaga atagagaaga atacatagca ggtgtggtct attcgccgac aacgaattca
    64081 ctctggaaag catcaaagtc aaatggagca tttcttaata attcaccaat taagtgtaat
    64141 gatccagtaa atcttgacag ggctttagta ggcactggct ttgcatatga catcaacaat
    64201 cgaaaagtgc aagcaaagat aattgaagaa ctacttccca aagtaagaga tttaagaaga
    64261 cttggtgcat gcgctgttga tatctgccat gttggaagtg gcgcacttga tgcttacttc
    64321 gaagagggag ttaatgaatg ggattatgct gcggcatcac taattgccca agaagcgggg
    64381 gccacttttt cagttactaa ggcaagagat ggatcttcaa ggagttcggt aattgtcgct
    64441 ggcccatttc ttcatgccgc ccttagcgcc caaattcagg ggcaatagcc cactttctgc
    64501 cctaattttt agcgatttag cacttagcct caaggtatgg caagatacgg cgagttgtta
    64561 ggccaatcct ttggaaaagg cctgaaggat aattaaagac taattgtgag gacaaaataa
    64621 tgaacgtgct cgatgcgcta gatgccgcct ctctccgtaa ggaccttcct aatttccgtc
    64681 ccggtgatga gttaaaagtt catgtccgcg ttattgaagg cagcaagagc cgtattcaga
    64741 tcttccaagg cttagtaatt gctcgtcaag gatctggagt tcgcgagact ttcacagttc
    64801 gcaaaatctc ctatggcgtt ggcgttgaga gaattttccc agttcatgca cctgtgattg
    64861 aaaagattga agtagtaaga aaaggcgaag ttcgtcgcgc caaactcttc tatctccgtg
    64921 atcttcgtgg caaagctgcc aagattcgtg agcgtcgtgg agcagatgac ctagaggttt
    64981 tgtattcaac ttcatcaact aatccagttg cagaagttgc tccagttgca gaggttgttg
    65041 aggtagctgc tccagaggct gttgtcgaag agagccttgc tccagagagc gcgcaggtag
    65101 cagaggaatc aactcctact gaaactgaac caactaagtc ttaaagccca gagtaatgtc
    65161 gcctaaagcg aagggcagta gccttcgcga gctgcctatt ctcatcattt cggctctagt
    65221 tctatcaatc atcgtcaaaa cgttcttcat tcaattcttt tatatcccct ctggctctat
    65281 ggagaacact cttcaggtta atgatcgtgt gggagtaaac aagcttgggg ctctctttag
    65341 tgatattaaa aggggcgaag ttgtagtttt tagagatcca gctaattggc tatcgccaaa
    65401 ttatgatgac acatcgggtt ttcgcaaagt aataaaggac tctctagttt tcgttggagt
    65461 tcttccagat ccctctaagc aatatttaat caagagagtt atcggagttg gcggcgataa
    65521 agttcgttgc tgcggtaaag atggaaaaat tgaagttaat ggcgtgagta ttaatgagcc
    65581 ctatatctat gaaggcgata agcccagtga ttcagaattc gaagtagaag ttccgcaagg
    65641 atttatctgg gttatgggag atcaccgcgg agctagcgca gattctcgct tccatactga
    65701 tgatcccaat aaaggaatgg ttcccctaga taaagtcaca gggcgggcaa catttattat
    65761 ctggccattt tctaatttgg cagtacttga aaagggcgaa gatctctcaa agattcctgt
    65821 taaaagctct aagtaattta tgattgaatc aaaacttatt caatcaggat taactaaaat
    65881 tgcaggagtt gatgaggctg gcagaggggc gtgtgctggg ccgctagtga tagcagcagt
    65941 gatactaaaa gatataactg atgcgaagct agctaaggta cgtgattcga aagagctaaa
    66001 gccaggaatg cgcgaagagt tatttgaagt aatagttgat cttgctcagg attattcaat
    66061 tattgaaatc tcggcagcag aaattgatgt tattggtctc cataaggcaa accttgaggg
    66121 aatgcgcagg gccatcaacg ctcttgaagt tgagccagag tatgtcttaa ctgatggcta
    66181 tgaaatatcg ggccttggct ctgaatctct agctatctgg aaaggagatc aagttgcaat
    66241 ttcaatatcg gcagcatcaa ttcttgccaa ggtctatcga gatcgaatta tgattaattt
    66301 agatcaccaa tatcctggct atcactttgc cgaccataaa ggttatgtca caagaaccca
    66361 tgagaggtct ttgaatgaat taggcgttac tgctattcat agaaagtcat atgcaaacat
    66421 tcaagctcta attaacaagt agggttattc acatctaact aataatttaa aaggttttta
    66481 ggcctccaac tcttattctc tagcaatgat agacatggag aatcagacaa atgatgattt
    66541 agagattcag gcccgcttaa agctttttag cgttatcgag ccaggcagca ttagctggtc
    66601 ggttgagtta aaacgttacg gcgcagttga attggtagag cgaattattc atggtcctta
    66661 tgagaagttt ggtagagaat ctgaggggta tcggcagcta cttcgagata cttctattca
    66721 aaagctttgg gaggagattg aaattgcgga tgcgagattt attactccaa attcaaagca
    66781 ttggcctacc tctctaaatg acttgcgcaa tccacctatt ggcttgatca ttaaaggagc
    66841 caatctgcca gcgcaatcta tctccatcgt tgggacaaga aaaccaacgc tctatggagc
    66901 aagggttgct agtgaatttg caagcggctt tgcagatcgt aattgggcgg taattagtgg
    66961 gggagcctat ggaatagaca ctcatgctca tcgtggctgc ttagctgcag agggttcaac
    67021 atttgccgtt cttgcatctg gagtatcagt taactatcca gctggaaaca ataaactttt
    67081 ctctgaatta caagaaaatg gcgcacttat ctctgaggta atgccaagtg ttggagcaag
    67141 agcggagaga tttttaacta gaaatagatt aattgccgct ctttcaaagg caaccatcgt
    67201 tatagaagca gcatttagaa gtggctctct tagaactgcc cgtgatgctg ccgagataat
    67261 gcgccctgtc atggccgttc cgggaccaat tacctcgcct actagcgatg gttgtcatcg
    67321 cttaattggc gagcgattag ccgagatagt cacttcagtt cctgatgcgc ttgaattgat
    67381 attgccaatc agtggccaaa agacgggtac tcttagcggt gatgacacat aaagctgcct
    67441 ttgttgcaat tgttggcagg cccaatactg gaaaatcaac acttgtaaat gctttagttg
    67501 gcgagaaaat tgttattacc tcacatcatc caaataccac gcgaaacgcg atacgaggaa
    67561 ttgttacccg tcctgacttc caattagtag tagttgatac ccctggagtt cataagccaa
    67621 aaacacttct tggaggggct cttaattcaa tagttaatga gaatatggaa tctgttgatg
    67681 caattgtgct ctgcatccct gcagttgagg ccataggagc aggtgatctc tttatcgcta
    67741 aagagatggc gcgccatcga agcgctaaga aaatttgcgc gataaccatg attgatatgg
    67801 ccaagaaagc tctaatgcct gagcaattaa tagctgcaag taagttagct gaggatgctg
    67861 gctttacttg ggatgaagtc gttcctttat ctgcgaaaac tgatgatcaa gttcaattgc
    67921 ttatggatct gcttgcaaaa tatgccccag agtctccacc tttttatcct gccgatatga
    67981 gctctgatca ggagctaaat actcagattg ctgatctaat tagagaggca gccattgaag
    68041 atgtttttga agaggtaccc cactcaatcg cagtcataat cgatgagatc tccgagcgtg
    68101 ataagcgagt ggagggtgat actccatttt ttgatattca tgcatcaatt attgttgaac
    68161 gcgatagtca gaaggcaata atcattggcc ataaaggaga gagacttaag caagtaggaa
    68221 ctgtggcaag aggtgagatt gaaaagaagt taaaggctcg agtatttctt ggtcttcatg
    68281 taaaagttat ggctaattgg cagagcgact ctaaggcttt acttaagttg ggatttaccc
    68341 ggtaactact ggggtttcag actttttgct agcaaaataa gaaccaacta agactaatgg
    68401 aagaccaatc gtaatagcta gcgtcaactt ttcattgaga ataatgatcc caagaattac
    68461 agcaactgcc gtatttggat aaaccacaag agaagccctt gctgggccaa tttcttttaa
    68521 gaccataaag aaaatccaga aagcaagacc tgttgagaag attccaagcg cagcagttgc
    68581 actaatagct tctaatgatg gactcgaagt tggcaagtta aaggccgcaa atggagcaaa
    68641 gaaaataaga gcaataagca tcgataatcc attaattgca actccagaga tttggggagc
    68701 acgcctagta atcatattta ctgcccaggc atatgagata gatgcaacca ataccattgc
    68761 aattgcgatc gcgctatcaa agtcatttag tgactcaatt cctacaagaa gtgccacacc
    68821 gattaatccc atagcaatac caaagattct ggtgcgatga gctgccgttg aatcaccgct
    68881 gtggtgagca aatagcgtgg cccagattgg aactgttgaa accaagagcg caaccaatcc
    68941 cgatgagaga tctctttgcg cagtggtaat taaataccag gggattacca tttcgagaac
    69001 ggagtagaac aaaatatatg gccaatactt aagagctggt cggatagatt tttcatagag
    69061 cgctatggga attaaaagca gggctccgat agcaaggcga gcaaagacaa ttacaggtgt
    69121 tgaaagctct tcaactgcaa ctttcataaa taaataggga acgccccata gcaaaccaac
    69181 aagggcaaag agcgcccaac ctttacgtga catttacttt attagactcc gatataactc
    69241 gatggttgct tcggcaacct tatcccatcc gaagtgctct attgcgcgga ttcgccctgc
    69301 ttgaccgtat ttaacagata gtgatttatc tgacattaat ctattgattg cagatgcaag
    69361 agcgtcttca aattctgatg aattaggtga gtaatcaacc aataatccag tttcattatc
    69421 aactacaacc tcaggaatgc caccaacttt tgatgccaca actgcagttt cacaggccat
    69481 cgcctctaaa ttaacaattc ctagcggttc atagattgat ggacagagaa atacttgagc
    69541 tccggtaaga agggcggtta attcatcacg ctctagcatc tgctttatcc aaatgatatt
    69601 ttctcgctgc tttgatagct cagcaatgag ttgctcaact tcagcaccaa ttgctggctc
    69661 atcgggagat cctgcagcaa ggactagaga gtaatcttta ggaactcttt tccaagcacg
    69721 gagtaagtgt gcaaggcctt tctgtcttgt tattcttcca acaaataaag catagggtga
    69781 atttatccca tacttcttta acacttctaa ttttggatta ggggcaaagg ctttagtatc
    69841 aattccattt ctaattgtta tgactttatc gctatttatt tcagggtaag ccttcaagac
    69901 atctgctttc atgccatcgc taactgcaat gatggcagat gcgctctcat aggaaatctt
    69961 ttcagcccag gatgaaattt gatatccgcc acctagctgt tcagctttcc agggacgtag
    70021 tggttccaga gaatgggcgg taataacatg gggaattcca aatattttca tcgctaaata
    70081 cccggctaga ttggcatacc aagtatgaga gtgcgcgaca tccataccct ttagattttt
    70141 ggcaatatcg ctatcaagta acagggtttg cagtgctgga tttatcgtag aaaactcttt
    70201 gattagctgg tagttggtgg catcacttct tgctccaccg aagcaatgaa cgaagctctc
    70261 gatctctccg ctctgattta aggcagagac taggtttgca atatgaactc cggcaccacc
    70321 gtagatctca gggggccact cacgagtaat catggctact ttcaccttag cgccgctcac
    70381 ctgccaagag tatcgttagg catatggccc gcttagatct cccattagga atagtgctcg
    70441 ctggtggcga gggtaagcgt ttgatgccat tgactgcaga tagagcaaag ccggcagttc
    70501 cttttggtgg ctcttatcgt ttagttgatt tcgttctctc aaatcttgtc aatgcaggat
    70561 ttttaagaat cgcagttctt acgcagtata aatctcattc ccttgataga catataacta
    70621 ctacgtggag aatgtcgaca ctacttggaa attacatcac tcctgtccca gcgcagcaga
    70681 gacttggtcc acgttggtat accggaagtg ctgatgcaat tttgcagaat ttcaatttaa
    70741 tccatgatga gaacccaaaa catgtgattg tctttggcgc agatcacgtt tatcgcatgg
    70801 attcaaatgc aatgtatcta agtcacttgg aaagcggagc tggggtaaca gttgccgcaa
    70861 ttcgagtaaa gcgatcagag gcatcagaat ttggagtcat cgaacttgat agcgatggcg
    70921 ttactataaa agcatttcat gaaaagccta aagatcctcc agctatgcca ggtcatcctg
    70981 atttatgttt ggtttccatg ggtaattaca tcttccgtcg cgacgccatg gaagaggccc
    71041 tgattcttga ctcggaagat gttgactctc gtcatgactt aggcgcgaac ataattccgg
    71101 ctatgacaag agcgggcctt gctaaggttt atgactttgc aaataatgtt gtgccaggtg
    71161 agactgagcg cgataaaggt tattggcgtg atgtgggaag tatagatact tattttgaag
    71221 cacatatgga tttagtttca ccactaccta tttttaatct ctataaccga cagtggccaa
    71281 ttctcaccaa tccgccatcg ctgccaccag caaaattttc agagcgagga attgcctttg
    71341 attcagttgt tggcgcggga tcaattatct ctggtggtga attgcgtaga accgttgcct
    71401 cctatgatgt ttatgcaggc cgtgactctg tcgttgagag ctcagtgctt atgccagcgg
    71461 tgcgaatagg ggatggagcc cgagttaaaa acgcaatctt ggataagaat gtaatcgttg
    71521 aacctggggc cagtattgga tttgatttag agagagatcg ccagcgcttc acagtttcac
    71581 ctggcggaat agttgtagtt ggaaaaggcg tagtagttac gcggtaattt cattggtaat
    71641 tttgcaatgc gctagaatct cctcctgagt cagagctgat tcaaatccaa ttatctaatc
    71701 ggagttattc atatgcgttt cggagtcctt actggtggcg gagattgtcc cggacttaat
    71761 gcagtcatta gagcggttgt acgcaaaggc gagaaagaat atggctatga gttcatcggt
    71821 tttcgcgatg gttggaaagg tccgctagag gggcttacga tgccgctaaa tattgcggca
    71881 actcgaggaa ttctgccacg aggtggaaca attcttgggt catctcgcac taatccattt
    71941 aagattgaaa atggtgtaga gcgcataaaa gaaaatcttg ccaagatgaa tgtggatgct
    72001 ctaattgcaa ttggtggaga agatacttta ggggtagcta caaaattaga tgccttaggt
    72061 gtaaaagtta ttggagtacc taagaccatc gataatgatt tgaataatac tgattacaca
    72121 tttggttttg atactgccgt aaatatcgca gttgaagcaa ttgatcgtct tcacaccact
    72181 gcagagtcac atcaccgcgc acttatcgtg gaagttatgg gaagacatgc gggctggatt
    72241 gcgctgcatt ccggacttgc cggcggggct tcatgcattc tcatacctga agttcccttt
    72301 gatatcgaca aagtttgctc atatgtgaaa tctagatttg aacaatctta tgctcctatt
    72361 ttggtagtgg cagagggagc tcttcctaag gatggcgatt taattactaa agattccacc
    72421 aaagatgctt ttggccatgt caaactttca ggaattggtg agtggctagc taatgagatt
    72481 gaaaagcgca ccggagcaga ggcgcgcacc tcaattcttg gccacattca acgtggggga
    72541 acgccaacgg cttttgatcg agttcttgca acgcgctttg gccttcatgc cataactgct
    72601 gctcatgaag gagattgggg caaaatggtg gcacttcatg gaacggcaat taagcgagtt
    72661 ccactggcct cagctactga gaagttaaag acggtagatc cagccctact agcagaggca
    72721 gaagttttct tcggataatt ggccgacgcc gattgcccta agttgcctac cctttgacca
    72781 tgtcaattga ttctctcttt gatcccgccc ttaaggcagc gcagcagcct aattggccag
    72841 atcgctctga attagagttg gcagtaaagg aattaaaatc gctaccgcct ctagtttttg
    72901 ctggtgaatg cgataactta aaagagaaga tagctctagc cgcagatggc aaggctttta
    72961 tgttgcaagg tggagattgc gcagagactt ttcttgcggc aaccgcagac tcaattagaa
    73021 accggatcaa aactattctt cagatggcag cagttctcca atatggagca agccttccgg
    73081 taataaaagt tggtcgaatg gcaggacagt ttgcaaagcc tagaagtaat gatctagaga
    73141 ctcgaggcga ggtttctctt cctgcatatc gcggggatgc tgttaatgat ctggaattta
    73201 ccccagaggc tcgtcgccca aatcctgctc gactagttaa ggtttatcaa acctctgcct
    73261 caacattaaa tctagtgcgt gcctttactc aaggcggctt tgcagatctg cgtttggttc
    73321 acgagtggaa caaaggtttt gttaaggact ctcgtattgg tgtgcgctat gaagctatgg
    73381 ctaatgaaat tggaagagct cttgaattca tgagatctgc agggattaat cctgaggagt
    73441 ttaagactgt tgatttttac tcaagtcatg aggctttaat acttgagtat gagaaggccc
    73501 taacaaggat cgattcaaga actggtaatg cctacgatgt ctcagctcac tttgtttgga
    73561 ttggcgagcg cactcgtcaa ttagatggag cacatattga ttttgccaag aaaattaaaa
    73621 atccaattgg agtcaaacta gggccaaaga caactgctgc gagcgcacta gaaattatcc
    73681 aggcgcttaa tccagataat gagccaggtc gcttaacatt tatcaccaga atgggcgcgc
    73741 aaaatgtgcg tagcgtcctt ccaggaataa ttgaagcggt aaataaatcg ggcgcaaaag
    73801 tactttgggt ctgcgatcca atgcatggca atacttatga agctccaagc gggtataaaa
    73861 cccgtaaatt cgatgatgtt ctagatgaag tgaagggctt ctttgaagtt catcaaaagc
    73921 ttggaactca tcctggggga attcacattg aattaactgg cgatgatgta actgaatgtg
    73981 ttggtggagg cgaggaaatc tcgcatgaag atcttgctac ccggtatgag agcgcttgtg
    74041 acccaaggct taaccacaca caatctttag agcttgcatt ccttgttgcc gagatgcttc
    74101 gcgatcgttc atagtatttc caaacataga ttaggacagt agggtttgcc cgtgctgctt
    74161 cgtaccactc ttaaaacccc agtaggcgct ctttctttaa tatcgcgcga acatatcctt
    74221 attgctgcgg gctttacatc acaggatgaa cttctatcct cgatctccaa gcaggatagg
    74281 cagttagaga gtaagaaagt aagtcagatt ccaataattt cagatttggt ttctgattac
    74341 tttgatggag acttgagagc ccttgatggg attaaggttg atcaagaggg tgagaagttt
    74401 tcacaatcag catggcgggt gatgagaaaa atagccccag gaagaactct gacctatgcc
    74461 gatttagcaa aacgagcagg ttcagaagat gcggtgcgag cggccggaag tgcttgcgcc
    74521 cgaaatctga ttgccctggt tgtcccttgt catcgaattg ttaaaacagg tggggcgcta
    74581 ggaaattatg cttatgggtt gaaatataaa cagtggctac ttagccatga aggcgcgctt
    74641 taaaagctcc tgcaaaatag cgcaagctct ctcatttgcc tcatcatcaa tatccatatg
    74701 cgttactaat ctaaggtagc tctttcctag cgcgcttacc aacagtcctt tttgttttgc
    74761 atccgaggca aattcaagtg cagatacatt tgctccggag aggtccagtc caacaatatt
    74821 tgtctctaca aaatctggat tgatagtcga aggtgcaacg cttgcgcagg ctatagctag
    74881 ctgcttagcc tttctatgat catcgactaa tcgagagatg ttgtgatcta gtgcgtaatg
    74941 accagcagct gccagaattc ctatctgtcg cattccagcg ccatatcgtt tacgccaaac
    75001 tctagaagat gagatgcgct ctttacttga gatcattaaa gatccaacgg gagctcctag
    75061 cccctttgaa agacagacgc taatggtttc aaagtgcttt ccatactcaa gtagagaaac
    75121 tccggatgca acgtgagcgt tccagattct tgctccatca aggtggagaa agagccccaa
    75181 ctcttttgct ctcctgctta gaagggcaat ctcctcaatt ggttgaatgc ttccaccgcc
    75241 aaagttatga gtgttttcaa ttgcaatagc tgtagtagaa actagatagg ggccagagtc
    75301 aggttttgct aaatctagaa cttgagctgc attcaatatc ccacgatcgg caagccaggt
    75361 ccttgttgta attccgctaa aggtcgctgc cgctcctagt tcagctctag caatgtgaga
    75421 gtttgtttca cagattaact cctgccctgg agataccagg gatcgaatag ctaactgatt
    75481 tgcaagagat ccacttgggg taaataacgc agcttctttg ccaaatagat ctgcaactct
    75541 ttcttcaagt gaattgactg ttggatcatc ggagtagaca tcatcgccaa cttcagcact
    75601 tgccattgca gagcgcatct gtaatgaagg tttagttaca gtgtcagatc ttaaatctat
    75661 gaattctgtg ctatctgcca tgagctaagt ctttcagcta gccctaggcg atgaaaagcg
    75721 aggctgaact atggcaagca tggcaattag caccaaggtg ccaccgattg ctaaacgtag
    75781 agtaaagggc tccattccat aagcgaaaga aaatagagct gcaaaaacca cttccatggt
    75841 tagtatcact gcaaccttag tagttgaaat gcgagcttga gaccaagttt gaattacaaa
    75901 ggcaatcgct gtggcaaata cagctgtgaa gataacgact gcccaaactt gagtatcagg
    75961 gggagggttg aatccattta ttccagcagg tattgccgat agaaaggcgc agccaattaa
    76021 ctgcatgacc gttagcgcgt aggcatcaaa gttcttgctc caggcaccaa gcatgatgat
    76081 atgaactgca aataaaattg ccgagatgaa tactagaaac tctccaaaac ctacgctcca
    76141 cccatgaaca gagagaactg caagaccaac taccgccaaa agaatgaagc cccacattgt
    76201 cagggtaact cgctctttta gtaataatgc tgcgattaaa ggggtgatca ctacataaag
    76261 gccagtgata aatccagtga tagcaggagt ggtgcgatca agacctagag tctgaaaaat
    76321 gtaaccagag cccagggcca atccaattat aaaaccttta ctaactagct ctttagtaaa
    76381 gagttttaat accttaggct tgataattat cattgccaga gctgcaaaga taaatctcga
    76441 agtcaggaag ctatagacat cctgctgatc caaaatgtcc ttcatccata caaaggaaat
    76501 accccacatt gcagctactg caagaagagc ccagggagcc aatttaagtt gcattattta
    76561 tctctcatct tcattagcat cgctacttct cttccatgct cgggttcttc accaggggtc
    76621 tctagaatca gtggagcatt aacaatagcg gggtgagcca gaagttctgc aaatggcttc
    76681 gttcctatct ccccatctcc aagattttga tgtcgatctt taagagagcc aagaacatcc
    76741 atagagtcat tggcatgaat tagttgaatt cgctcagagc ctgcaacctc caccaacaaa
    76801 tcaatagttg cgctcatccc acctttgctc tttatgtcat gtcctgctgc aaagacatgg
    76861 caggtatcta gacacactcc cactttagga tgccactcaa gggcttcgaa ataattattt
    76921 aaatcctcta accgtttaac tagagattga ccttgaccag cggttggttc aagcagaagc
    76981 caaggatctt gatcatctag ctcgctaaga attggcatca tgccttcatg aatctgcttc
    77041 catgccttct tgatatttcc ctcatcgaca gcagaacctg tgtgaattac tacacctcta
    77101 gcctttatct cccgtcctcg cttgagggag tattttgtgg atgcaagaga gttcttatag
    77161 gtgccttcgg tcggagatcc taaattaatt agaaatggcg cgtgaatata agtttcaata
    77221 tctagttcac ttgccttttc aatgaacgct tgatcagcat cactatttgt ctcaggcatg
    77281 gcccaacctc tggggtttga tgcaaatacc tgaattgcct ctgcctttat tgttttggca
    77341 tattcaatag ctcgctttgc cattccacca gaagttggca catgagcacc aattcttggt
    77401 tttgctgctt tcggcactaa atcaccctac agtgattgtg accgttgagc cacgttttac
    77461 tttatctccc acttttggtg agatattggt gacaactttc gtctttttat tgccaacttt
    77521 cttgacaact acctttaagt caagatcttt aagggtatct attgcttttg cttcgcttag
    77581 ggagaagaca ttggggataa aaacaaattg agatcccttg gatacagtta aagttactac
    77641 gcttccttta tcgatatctc caccgcttgg aatctgacta acaactgctc caattggcag
    77701 atcttcacta aagacatact tagtttcaac atcaaagcca gcttcagtta attcattaag
    77761 agcttgttcg ccgctctttc cttgatagct ggaaactcca acttgctcga ctccttttga
    77821 aagatagaga gttacttggc tatcacgctt gatacgttcg cctgctgcag ggctacttcg
    77881 cataataaag cctttgggaa attcactcga aaattcttca ataatctcac cgacgactaa
    77941 gttattatca ctaattagtc cttcagcaat ttcaattttg agtccttgaa gagttggaac
    78001 tatgtaacgc tccttaccct ttgaaattgt cacctcaact gttccatctg gtgaaatgcg
    78061 cccgccgcca gccggacttg atttaatgac tctattttct gggatatctt cactaaattc
    78121 ttctcgctct accttcagat caaggccaag gtctgccagc tcactagttg cttccttaac
    78181 agtaaggcct gcaagagatg gaacaattac ctttgaacca ggacctacga ttacatacca
    78241 aatgccaatt ccaagagcga ttgcgattag tgctgctatc tgtcgatttc gtttaactcg
    78301 tttagagagc gccttcttag taattgaaga actcttctct tttttcacgg cagcctcctg
    78361 gtttttggga atttccactt cacgatcttt atctctacga aggcgctttg cgccatctct
    78421 ttttcctact tcctttatag caagtggtgg caggtcaagc tcaagactta attgcctttt
    78481 gcgcggatct aacttttcag agagttcgcg cagtttctca agcattacta ctgcatccct
    78541 tggacgttta tcaggatcta tgtcagtagc ggataggact agctgatcaa tttctactga
    78601 gagtccgggt tttagtgttg aaggagcagg tactcgttcg tttacatgct tgattgcaac
    78661 ctgaactgga tcctctcctt gatagggctt ctggccagtg agaatttcaa agagaacaat
    78721 tccaagtgag tagatatcac ttctagcatc tgcaactcct ctttgaacct gctcggggga
    78781 gaggtatgaa acacttccta gaatcacact ggattccgca gtcatagtgt ttccaagaag
    78841 tgcgcctcga gctaaaccaa agtcagcaat ctttatgcga ccttcttttg agactaagat
    78901 gttttcaggt tttatatctc tatgaacaat tcctaactta tgtgcagcag caagagcgct
    78961 tgcaactggt gtaatgatgg ataaggcacg ttcaggagaa agtgagccct gctcgattag
    79021 gtaatctctt agcgttgcac cttcaattaa ctccataact ataaagacgc aaggagatcc
    79081 accttgattc cacccttgat ccaatactgc aacgatattg ggatgagaaa gtgatgcagc
    79141 agctttggcc tctcttataa atcgagagac aaattgctca tcttgggcaa gatgagaatg
    79201 cattattttt actgcaactt ggcgatctag tcttaaatct atcgcggcat aaattgttgc
    79261 cattccgcca gagccaacaa cgcgggttag ctgatagcgc ccatcgatga tttctccaat
    79321 taactccgac acgtggaaat tttagatgaa agttgctcca tcaatgcgaa tagtggcgct
    79381 atcgcgagtg ttgtctgcaa caaagtggta attctctctc atctgccaga tctccatctc
    79441 tttttcaatg gattcaccat ctctttgcaa gactctttgc aagccaacct gagcagagat
    79501 atcaatccaa atcagctcat ctagatactt tctaacaacc ctctggcccg aacccacacc
    79561 ttccagaatc aatatctctg gaaaagtgaa gtccagcagc gggccaaact ttgattcaat
    79621 ccaattatat tttcgatatt gaaaactctt tcctttagaa actggggagc agatgcttat
    79681 atctaaaact ttacctagcg ctggagttag cgagttttgc caaccatcat agagatcatc
    79741 catatgaaca atctcaacac ttgactcttg gatgttctct ttaaggcgat tggctagagt
    79801 tgttttacca gctccagcag gtccatctat tgcaactatt cgtccaacat tgcctattgc
    79861 tctacccttt ttaatgcttg tgatgatttc agtaagtgaa ggctgattca tattagattt
    79921 gatcagcact cttagataat ttcttccaac gctctagccc aaagacggcg atgattgtaa
    79981 agacgccata gagcaatgca gtgaaccaat agccttgatt tgcatagtga taagttccaa
    80041 aaatatcaat cacaatccag gcaatccaag tctcatttct ctgaagcacc attgcagtct
    80101 gtgccagagt gcttgagata agcaagaagg aatctggcag tgttgcagca gcgccgatgc
    80161 tttcaagtat tggcgcagta agaagccaag atgccactaa agctgtaagc cagattaatc
    80221 tctccttgtt attcatatac ctaggtataa cgccggacct attccagcca agccaacccc
    80281 agattcctgc tgcgatgaag acaaattgaa gtagtgcgct agcaattaga tctacttgat
    80341 agaagaagat tgcataaagt gatgcgctaa cgaggtacca gggccaggtc attcttactc
    80401 cgcgcgcccc gagaagtaca gccgtaaatg aagtgattac tgcgcttaac tccaaggccg
    80461 atacttcata gccccaagct gtgaaaatgg taaacatgat taagctccct tccaattcgc
    80521 attaccgaac tggtctttcg gtcgaccttc gaagaggtcc tctcagccaa cttttggctc
    80581 ccgattagca gaacaatagc attagcccat gttgaagttc ggttattttg caatgctcac
    80641 cttcacagtt ctcggttcat tctggctgga gatttttctt aaagttggag tattgcgaag
    80701 aattaagcga gcgatgttag caattcttcc tactgcagct attttcttaa cttgggatta
    80761 ttacgcagtt acctctaggc attggttctt tgatagagag caggttgttg gaatctttgg
    80821 acctgctggg attccgcttg aagagttcct tttcttcatc gtagttccgc tagctgcttt
    80881 aatgacaata gaagcagtga gacgggtaaa gcctcattgg aattatggtg atgagcgatg
    80941 agttacaccc aactagccct tctttcagtt ccactcgtta ttttatttga tttttatata
    81001 actaaatcta gagttttatc aagaaagagt ttctggaacg cctacgcaat catgcttttt
    81061 tttcaaatca ttacaaattg gtggttaact tctagaggca tcattactta tgaccctgat
    81121 gtaatagttg gggtgcgaat tgcctctgct ccagttgaag atctattctt tggattttct
    81181 atgattgtta tggttatctc gcagtggatt ttttggggaa gaaggggaat acaaagaaac
    81241 tagcgagaag cgagcgctga aaaataagca ggaagcgccg ttttaactcg ccgaaaatta
    81301 gaagtcttag ctcgcttggt aaaaatttga taatcaattt tctcaacctc atcgacaatt
    81361 ccgcagtaga gctcacttgc tgctctaatg cacgcccttg catccgggct tagcagctca
    81421 attccagcat ctgcctcgct ctgtagttgg cgcacccttg caatctgaaa cttgagcgct
    81481 tggataattt cagggcgaac ttgccttgcc tctagcatct cgcgatctac gccaaatgag
    81541 gccagctctg ccattggtag atatattcgc ccgcgttcta gatcctcgcc tacatctctg
    81601 atgaagttgg ctaattgaaa ggctattcct aacttctcag ccggagcgag cgcctcagat
    81661 ttttttccct caactgttcc cagaacatga accatctgga gcccaattac tgcagcagat
    81721 ccatagacat actccatcaa gtcttcatat ctttcgtact gggttacaga taaatccatt
    81781 gccatggagt gaagaaatgc ttcaaagtaa gaaattggaa tgttaaaacg ctgcactgta
    81841 tcaaccagag ctcgtccgac atgatcgcta cttcgcccca tagaaatgtc ggataagagt
    81901 tgatcactcc acctgccaag ttcatctact ttttcttggt gagtaagagt tgatgccaga
    81961 tcatcgacaa tctcatcggc ataccgagca aatccgtata gcgcatgaac atgtgggcgc
    82021 ttagcttttg gaagaaggag agtagctagg tagtaagttt ttccatgaag agagttgagc
    82081 cttttgcact ccaaataaga tttgcgtagc tcaggatcat ggattccagc agcgcttaac
    82141 tcatccatat ttactttact ggtccaacaa ttctctctgc tgcaagtctt cctgagatta
    82201 gaaccatggg aactccgact ccaggttggg taccacttcc agtaaagacc acattctcat
    82261 atccctttgc catattactt ggcctaaaag gtccggtttg taggaaagta tgcgcgcttg
    82321 caaatggcgc acctctttcc atacccatct cttgccaatc gaggggagtg gttacatgtt
    82381 ctacctcaac tgagtctgca aaatctttat agccccgctc ttgcattgtg gcatacattt
    82441 tatctttata aacgtttttc atcttcttcc aatcaatatc agcatcaaga tttggcgtag
    82501 gaaagagaat gtaataacta tgttttccac ttggggcaag gcttggatca tcttttgaag
    82561 gtagagtaac caaaaggctt gggtcgctca tcaattgctt tttagtaatt agctcatcaa
    82621 agactccatc ccaggagtgg ccaaagtgaa tattgtggtg agctaaatgg tcataactct
    82681 tggaagagcc agccaagatg gttacacaag aaggtgagta gttgagtcgt ttaaccgata
    82741 gcggagtttt tcctagtaaa tctcgccacg ccacaggcaa atcaggattt agcaccacaa
    82801 catcacattc aattctctgg ccatcactag ttattacagc acttgcccta gatccactct
    82861 tttctacctt tgtcactgtg gtgttatatt taaaagtcac gccatgtttc tcggcagctc
    82921 ctgctagcgc tcttggaacc gcatgcattc cacctttagg aaagaaaact ccattaactg
    82981 aatccatata ggcaataact gcataaatag caagtgcttg ctggggcgaa accccggcat
    83041 acatcgcttg aaaagaatag acgcgttgag ttctaggatc ttttaggaat tgagagactt
    83101 taggagcaag ctttctaaag ccaccaagag caattaatcg agcaagatta ggagttagca
    83161 ggtttagggg agagtcaata tttctatcaa taaagtcatt catctcatac ttatataact
    83221 tggtaacaaa ttcaacatat cgtcgatagc cagcagcttc atctgcacta atattttttg
    83281 caatctctac ttccatctgt tctgtatttg catgaacatc taattgagaa ccatcggcat
    83341 aaaaggctcg atatagtggc ttaagtggca tcagctcgag ccaatcttct attttctctc
    83401 caacgcatgc cagagcatcg gcaatcagat caggcatagt taaaacactt ggtccagtat
    83461 caaatgaata tccatctttt ttcattaatc catttcgacc accaggaacc gcttcacgct
    83521 caatcacaat tacttctcgc ccagctccgg caagccttag cgcgcaacta aggccagcaa
    83581 gtccagcacc gacaactact actcggtctg tttttccctt aacttttcgc atttaaagac
    83641 tccgttgcgt tgcaatagtt gccatctctt ctaatacaga gagaatactt gtctcaactt
    83701 ggctatctcg caacgcagaa atggctctat cccgaagctc tgtgatgagc ttctcaactt
    83761 gagcgaccgc tccagagcta gaaataatct cttgaagctt aagaatctgt gttttatcaa
    83821 gtccttcatc tccaagtgag gcagttagaa gctctcgctc ttgtttgcct actgcctgca
    83881 tggtgagggc aattagcacc gttcgctttc cctctcttaa atcatcacct gctggttttc
    83941 ccgtgacctc tggatctcca aatacaccaa gtagatcatc acgtagttga aaggcttccc
    84001 cgagtggaag tccaaatgat gagtaggcgc tcattagttc cttttttcca gagagcgctg
    84061 cgccaaagtg aagtgggcgt tcaattgagt atttacctga cttatatcga gcaactttta
    84121 gagatctatc aatactagtt gtggcaagtg cgccctctaa aacatctaag tactggccag
    84181 ccatcaattc atctctcata tctgcaaata ctgattgcac ttgaagaaat tcatcgccgc
    84241 taattccaga ttccaccaat aagcgatcgg cccagaccag agccaaatca cccataagaa
    84301 ttgcggagga acttccaaat ctttctgagc tgcctttgta agaagaatct tggtgatatt
    84361 gctgaaacgc tttatgtatt gctggtttat ttcttctagt gtcagagcca tccatcacat
    84421 catcgtggat aagtgcgcaa acgtgaacta actctaagga tgtgcaggca cgtagtatct
    84481 cctcgcgcgc ttctgctccg cttcctaaat aacctaaata ggcaaagatt ggccgaaatc
    84541 tctttccacc ttgggtaata aaatctgaca ttgcctttgc tactggcact agctctgggc
    84601 caatatcaac aagcgcagcg cttcttgcaa cggtaaatga ggccaaggtc ttatctatct
    84661 cggcgcgaag ctgggatagg tcaggagatt tcacggcgca acggtaccct cccgctatat
    84721 gaatagtgcg cctaccttct ccgttgagtt ctttccgcct aaagatcagg ccggagagga
    84781 gcgtctctgg agcgctctaa aggctcttgc agtctataaa cctgattttg tttcagttac
    84841 ttatggcgca ggtggttcaa cgagagatcg cacagtaaga attacctcag agatcacagc
    84901 aagaagtgga atttcaacaa ttgcacacct aacctgtgtt ggatctagta aagatgaatt
    84961 aattgagatt ctcaatcaat acaaagcctc tggaattaag tcaatccttg cacttcgcgg
    85021 cgatcccaag ggtggacctg gcgtgaaatg ggagagtaca gctggaggct ttgatcacgc
    85081 agaccagtta gtaacgcttg catcaagtat gggctttgaa gttggagttg cagcatttcc
    85141 tgatggccat ccagcatcaa tgggaaattt tgaacaagat ataaaagtac ttcttgagaa
    85201 ggaaagatgc ggagcaagtt ttgctacaac tcaattcttc tttgatgtta agggctatat
    85261 caacttagta gatgagataa gagctagagg ttcaaagctt gatatctatc ctggaattct
    85321 tcctataact aattttgctc aattaaagaa aatgagcgaa cttgcaggct cgccaatccc
    85381 aagtgaagtt caaagaagag ttgaggcagc aggagatacc ccagttgatg tggttaaagt
    85441 cggtatagat attgcaagtg agctatctaa aaagcttcta gattatggag tgccaggtct
    85501 acatttttat actatgaata gcgcagctcc aacgattgaa attattaaga gaattggact
    85561 tagatgaaga agatagaatt ctttaattcc tggtccagcc ttcatggcgg agcagagata
    85621 aaaggaattg tcagagcatg gttagaaatt tcattcctgc tctgtaaacc tctagcagtt
    85681 ttaagagtta cccctaattc tctatctatc tttggtttac tgctaggcat caacttggta
    85741 gtaaatattg aaagtaattg ggctatagcg cttctagtat tctccttgat tgcagatgga
    85801 attgatggat ccttagcaat acttactgga aaggtcagta ggtggggagc gctacttgat
    85861 ggctttatcg atcgaattgt tgaagcgctc tgggccttta gcctctatct acttggagct
    85921 ccctatcaac tggtcttaat cgcttggctt gcagcatttc ttcaagaata tatgcgctca
    85981 agggcggcat cacttggcgt tactcaatta ggggttgtga caattggtga gcgtcccatt
    86041 agagccagca ttttatttat tgctctcgtt gcaagagctg ttgatatgga tctagcaacg
    86101 ctgctctctc agatctgggc tgtgcttcag gtaatctctc tatttactgt tctaaatttt
    86161 cttcgaccgc ttctgcaaca atctcagcgc taattccaac taaaggtaga cctccgccag
    86221 gatgagttga accgccaact aagaataagt ttttaatctt ggagcgatta cgcgccctcc
    86281 aaaaggccga tcttgcgcca ttgctagatg tgccataaat agaaccccca ggggcgttaa
    86341 cgtttctttc taaatctgct ggagatctat attccaaaac ttcaattcgg gagcgaacat
    86401 ctagtccctt tgcctccatt agattaataa tgtgctggct atattgcttt gagcttttct
    86461 catcccaatt aaagccatct cttggttgat gacgaggggc attaattagc accgaccaac
    86521 tctcatccac tccagctcgc ttcatcttag agtcatttgg attgcagata tagatagctg
    86581 gatcttctac tggccttctc ttttcgaaga tagaatcaaa ttcagcatca taatctttag
    86641 ggaaaaatac agtgtggtgg ccaagctctg atgaaccacg aagcccaagg agaagtgaga
    86701 atccagagag tgatgcagtg gctttgttaa gtgagttacg ggcgcttcta gttctggaac
    86761 tcttttcaag gagctggtta taaactaaat ctgcatcagc gttggcaatt actacatcgc
    86821 tattatgaac ttggccatta gccaatctaa ctccaattgc aacgccagat ttagtgatga
    86881 ttcgatcaac ttttgtgttg agatgcagct caactcctaa atcacttagg cgagactcaa
    86941 gggatttagc aagcgtgcca actccaccgc taacatgcca agcaccgaaa gcctcctcga
    87001 caaaggcaat ggttaaaaga actgcgggga cttttctagg atcggagccg gtataggtgg
    87061 catagcggtc aattatcttt ccaatgtaga cattatcggt ataacttcta actaagtctc
    87121 gaagggaagt aaatggagcg attgttttta aatcacggag aagtgatgga ctctttagca
    87181 tctgaagagg ggagcggagc tcactttcaa caaaagcttg tcttgatgcg ccccacatct
    87241 tctcagccct agtcagtaat tgatgccaag catctcctgc ctgcgttccg aaactttcag
    87301 taatagctaa gactgtgccg ctatgtgaga gatttggaaa agttactctc ttgccatcat
    87361 caaagatata tgtaaaagag ggatcaactg ggtttagttg gacgagattt tctaaacgct
    87421 ttcctgtttt aacaaaaaga tctctgtaga cagctggaag ggtaagtagc gaagggccag
    87481 tatcaaatga aaatcctgag atatcttcag ttctacactt tcccccagca aaagaagctg
    87541 attcaaagac tttaacttta tgcccacgct ttgcaaggcg ggcagcagct gccataccac
    87601 ccatgccggc accaattaca atgacggatt ttgatacttt gctagttctg tgatggctca
    87661 tagtttccta ccgcgccaag aaagttcgcc tcgatacttc tttatccaag aaaacgatat
    87721 taagtagatc cagataagcg cactcaaggg atgaagaaga gaagtactgg gaatcgttct
    87781 tgtctttatc ccgcttaata ctcttgtcat aacaattgca aagtagagat accaacccga
    87841 gagctctcct gctaggccag ctataaaggg aaatatagat gtgagagtta gcaaagcaat
    87901 tgcaataagg gcccctaatg gatttacgaa agcgctccac tgtgacttgg tatagccttc
    87961 aattagctga ttcgcacttg aatacattcg acagtatgaa actcttgatc catctgcaac
    88021 gccgcctttt gcgccagttc tcactaaatt tctagcaaga aacaggtcat ctaggacttc
    88081 tgctttaaca cctctatggc catcgcaatc aaagtaagcc ttacgcttta tcaccataaa
    88141 ttgaccattt gcgacaacag ttgatggtct tggccacttt tgagtgattc gaagtggcag
    88201 agttgtaaac caggaccatt gaagtagagg ttgagttaac cgttctaaaa atgttagggc
    88261 aatctgtcga ggataggcag agatgaaatc ccaattccaa gctctcattg attcaatggt
    88321 gcttgctaca gcccttggat ctagcctcac atcagcatct agaaaaacta aataatcacc
    88381 cgttgcagct gtggctaatt gatagcaggc atagttcttt cctagccagc cctcttccag
    88441 ccctcttccc gcaattactt ttaagttgtt ttgggacatt ccaagtaata gctctttagt
    88501 gttatcactt gaggagtcat ctagggcaat tatttcaaaa ttacttaagt tagcctgctc
    88561 ttttagaaga gtgattaact caatgacatt ttcagattca tttcgcatcg gaattaatat
    88621 cgagactgat tgatcactag aggcgctatt tttactggaa accactctca gcgtaaagaa
    88681 attaaagacc gctaaaagaa gcataattgc aatcagaaaa gccgagacat ccaatagaaa
    88741 agtcattttt ccttaatctc ggcggccgta tttaactgag atgaaatacc agataactag
    88801 cgctcctagc gctgatcctc caaggaatgc cacaccggga cgatcgaaat ggaaaatgtt
    88861 aatcaccatg ccgccaatcc aactccaagc aagaaagaat tcaggaactg ctctacttga
    88921 accaagggag cgccgctctt taggtaggaa gatattgaga agggccatca accccattcc
    88981 agttaatagc cagccgaagg tattagagag tggaatttct ggttgaaatg gaacgcttct
    89041 gccacttacc tcccaactcc atcttccagc tgaaaccatc tgaggatcaa gaaatagatc
    89101 ccaggccata aggccatacc caccgacaat gaagactaag tttggagcaa ctcttctcgc
    89161 tgcgataaat actgagtggg ccatcatgat ccaagcaaat ggaactacta atggaacacc
    89221 aaatatctgg taaccaaggg atggggaata ctcataactt ccaaatggcc aggtagttcg
    89281 catgccaatt tgttcaacta atagggaata agtgaaagtg attagtagaa atctgattgc
    89341 ataagttagg ccataagcaa agagagcgtg gagcaacatc gctcctgctg cccaataaac
    89401 agttgcgata gttacaagtc gaagacgctc tccttcaatt aatggatagg agagttgaag
    89461 agctagagca acagttaata ctgcaaccag cgtaaaattt cgccaaggtg ttatcgtgcg
    89521 cctggaatca ggcttcgggc gataattacg aatcgacatg gcttaattgt gccttatagc
    89581 gagccattag atttaataag ggcaaagcgt gcaaacatct cctcggcata ccaagcatgg
    89641 cgatgcgtag cctttatcgc ctctctatga gcagcgccag agtaggcaaa attcttaact
    89701 gcttcgccag attcccagat agagaaagta ccttgtaatc caataggagc ttcaccgatt
    89761 ccaatagcag aaatcaatcc tggtgagcta tgaagacttg atgtaactgg gggaactgag
    89821 ctccagaaaa taaaattctt acgccattta atcctagctc ttgtgattgc aactattttt
    89881 ccgctccaat cactactagc tttaggaatc tgatcaaatg ggctcttctt tgaccaagcg
    89941 ccattaattg atatcggaga tagctggccg ctaaattgcg aatgagattt cttgttccaa
    90001 cgtttaatga tttttgaatt ggcaaactct gagtcttgat caactacaac tagcaatccc
    90061 cagcgaagaa catcggcatc ccttggggtg aaggtctcac cctttccagc tcctaatagc
    90121 ttccagaatt taacatcact tcttctagct agggctaaac gatctagtcc catatgcaga
    90181 attgcccagg ggatgtagcg agctttaatc ttccaaaaga aaaccagaat cactttgctg
    90241 ctccagccgc ttttttaatt tcatcgatta catctagggg gctatcccac atcggagcat
    90301 ggccggtatc agagaatatg atccatctag catgctttgg cgcaagggat ctctcctggc
    90361 agctagtctc aggaagagtc ctatctgaat caccaaaaat aattgtcact ggaattgaat
    90421 cagatatctg acgatcaaaa cgctttagta acatgccatc ccaagccggg taataacctt
    90481 ctgcgcttgc catggcaatt gctgcatcaa gacataattc ataagagaac tcttgccacc
    90541 gcgggctaac atcagcaaaa cctaatctgc gagcaatttc gaagtgaagc gctgttggag
    90601 caagcttaat tgtgtatcta gcaagaaatc gagctagcgc tgtgcctgga tatctagcgt
    90661 tataggggtt gagccaaagt ccagcaggtg cgatgcctac tagagatcta actctttctg
    90721 gagcaattgc agccatctcc attgcaatcc agccaccgag tgaattacct gcaagatgaa
    90781 atttatcaat agatgcagcg cttaactgct ccaacacttt aagtgccaag gagtgaggat
    90841 ccattaattc acctttttga taaggactct taccatgtcc tggaagatca agggtgacta
    90901 ctaagaagtc tttatctaaa gttgagcgaa tcggtttgaa agcattgctg gcagagccca
    90961 tgccatgaat tagaactagg ggatctcccg cgccactttt ttcaatatat agaggagaga
    91021 tcacagcgca attctcactc taattaccaa ctcttgcagt cttagcacct gatgcctgta
    91081 gtaattcttg ataagtagtt ggaaatactg cgtgtggatg acctgcggcg gcccagataa
    91141 cttgataatc atttaaagcc tcatcaatta aagtgatgct gggctcatta atccaaccaa
    91201 gtggagcaac tccgccaaca gagtatccag aaatctcctt aacataatct gcatctgctc
    91261 gatcaagggc tggcacttgt aatagatccg ccaccatctg cgtatcaact cgatggcgac
    91321 ctgaggtaat tactagaagt gatctatcat ctggcaactt aaacactagc gatgaggcaa
    91381 tctgtccaac ttcaatacct aatcctgctg ccgcttcagc tgctgttctt gcgctatcgc
    91441 taagaatttt tatctttccg gaaattccat gtttatcaaa ggcagcttga gcgcgcttaa
    91501 ctgcagcctt ttccaatatt gaatccatag ttggattcta gagagtgctt atcaattcct
    91561 taactagcga cttaatctga tcgcgaattg ggcggattgc atcgatgcct tgcccggcag
    91621 gatcatctaa tttccaatct agatagcgct tgcctggata gattggacat acatccccgc
    91681 atcccatagt gataacaaca tctgaggcct caacagcttc atcggtaaga atctttggtc
    91741 tctgcgcgct tagatcgata ccttcctctt tcattgcaag aaccacagct ggatttattg
    91801 aatctgccgg agcagaacct gcgcttctta tctcaaccca atcaccagca aggtgctgga
    91861 gatagcctga ggccatttga gagcgccctg cattatgaac gcagacgaag agaactgatg
    91921 ctctcttaat actcaccggg aaattctctc tctatttctt aataaagcta gaactaaaag
    91981 ggtccctatt aattgcgctg caataaatcc aggaagcgag gagcttgcaa ttccagcagg
    92041 ggcatcagta aagaatcggg ctaaagtcac tgcagggtta gcaaaggatg tagagacggt
    92101 gaagaaatat gcgccaaata tccatagtgg aattaatttc caaacatgct tcttccttgc
    92161 gctaaaagcg ataaagacta ggccagttgt ggcaattact tcaccaagta attgagcagc
    92221 cccatctctt tgaatcgaac tactactaaa taccacttca gcaaacataa gatttgcaag
    92281 aattacccca aagattgcac ctaatacttg agcaatcaaa tagaccagcg cctcaagagc
    92341 tttaattttt cctagcaaga gagcagttag cgtcaccacg ggattaaagt gagccccact
    92401 tagattaact gcgcttcgaa ttattaaggc taggaccgct gctgtcacag ttgcgttaat
    92461 caaaagcgcg agagcgctat cttgcgttaa tcgctcggcc ataaaagatg atccaacgac
    92521 tgcaagaagt agagttgcag tcccaaagaa ctctgcaagt gcttttgagc caatcatgag
    92581 attagccaag tatcaattag ggtaaatgtt gcgattgcaa agacaaagaa tgcaaagccc
    92641 ttcttaagag tagctggaga gatattagag ctatgtcttg aggcaaggcg cgagatgaca
    92701 agggccataa gtgcaacaac taaaggaatc gaccaattga cctcatccca atggcgataa
    92761 tgagcaaaga atccagttaa ggtattaatg gcgattataa atagcgaggt accagcagct
    92821 ttagcaggag ctacgttata gaagagaatc aagattggaa tagctaagaa accaccacca
    92881 ataccaaata gcccagtaat tgctccaata actagtgaga tagcgataag agcaagaggt
    92941 gaaactttgc gctcactaga ttccttggga gccggaatca gcattgaagt tccagctaca
    93001 acaagaacta aagcaaagcc ggtaagaatt gtccgctctg gaatttgtgg caggaaatat
    93061 gcaccggtga agttagtgct aagtcctatg gcccagataa ctagcgcctc tttaattagt
    93121 atctcgtttt ttctaagttt cgggagcgct ccagaagctg cgccaataaa gacaacgatg
    93181 agagcagctg tagttgcttg aactggatta aatccaaaga tgtagatgaa ggccggaact
    93241 gccaacatcg atccaccagc tccgacgtat cctaaaattg ctccaattgc gatcccggta
    93301 agaattgcca gtgctatctc cattagagga ggctacctaa gtcgaaagaa aaacccccgc
    93361 ttctcatact aattagcggg ggtttctgtt aagaattcaa ctgttgttca actagaggtt
    93421 aaagattccc tctgtaattg ttgggccaaa ttctccgccg cttccaacca aaacaaagat
    93481 aatcagagaa gcgccagcaa gggcgagatt cttcatgaaa cttgtcatct cagactgctt
    93541 tgcctgtgca tctgtaactt tccaaaagtc atgcatttta aatgctgaaa gaagaaggaa
    93601 aatagcgagt agtagcgcac ctaaatcagc ataaattcca agtgccacat atagaccgcc
    93661 aacaatcaac attaaacctg tgacgatcac agataacttg gctgctggaa ccttcttata
    93721 ttgggcatat cccgtcattg cctcaagctt tgttagatgg gctactcctg atgagatgaa
    93781 tagtagggag aataaaactc ttcccacgat taaaacgata tccatttaaa gcctttcgct
    93841 aagtgattaa cttccgtgaa gcgaaattgt aaatccaaaa gaatagaaaa gtgcttaatt
    93901 gagcgccagt tttgccataa aaaaagcgcc tcagccgaaa ctgaggcgct ttttatcttt
    93961 acttatggat ttactttagt aatacgtcca tcaagggctg ggatagtaat tgccttacca
    94021 ccagatgatg cttcagtaac tgcttcgatg aagacatcaa ctaatagacc gcgcaatttc
    94081 gccttagaag gagtaaagaa gccaacgtat ccatcgcctc catagatcat gaagtctggc
    94141 accgcaactg tgtactcctt agagtcttta gcaatagcgg ttccatcggc tttagtcaca
    94201 ctttgtacgc gcgagccaac agcttttgtg gtgtcaaaac taaatttgaa accagaaatc
    94261 tgtgggaagc gtccatcgcc tggccagtta cttacgccat tttcgagggc cttccagaga
    94321 ttctcaccag taattactga tactccaact gtgtttccaa atggatatac cgcaagggca
    94381 tcaccaagtg ttacatcgta tggagcaacc ttgtcagttc ctgggcgatt aagtgactta
    94441 tctgcggcaa cataagttgc agctgggaat ttatctctaa tgccgccacc gttgatgtat
    94501 gcaaagtcag ttccatactt ctttcgcatt gcatcagcag tgaagtttcc aattgccgct
    94561 tcgccagctc tttgaacctg tggagatcca ccttgtggga agatgccatt gactttgcca
    94621 atcttcacat caagcttggc atttagctga tccttatatt tcttaactag cgctgcgcca
    94681 tccgcatttg gagtaagagt tgcgatgttg gcgcgagtta ctaactcaac tgttgtgcca
    94741 cgaacgcgac gtgaactatc taggcagaga gtgactcggt tatattcctg accagcattt
    94801 ctagtcattg caataacggt tccattgact gcagcattgc cagcaagagc tgaggagtag
    94861 gtctgatggc tatgaccacc aaataccact gctgcgccct tgatctgctt tgctaaatca
    94921 atgaggcgac cttttgcttc gccatcacgg ttttcttgcc atccttggtg aagaattgca
    94981 acgacaaagt ctgctccttc ggccttagct gctttcacag ctgcattgat gccagtgaca
    95041 tcaggtgaaa taactagttc ttttccaccg aatgagagat tgccaggaaa tatctgctcc
    95101 ttagtatctt cagtatttcc agcaacaaag ccgactttgg ctccgccgcg ctcaagaacc
    95161 aagtaactct tagctgcctt attaccactc tttaatggct ctagggtgtt gtaattcgat
    95221 gctacccact ggaaatcaga tgcaccaata attttctgaa catgtgcgat atttctatcg
    95281 tgttcgtgat taccaaatcc agaaacatca aacttcatta gattcataga ttcaatagtt
    95341 ggcaattctt caaactggct tgaaattggt ggagcagctc cgatgttatc tccagcagag
    95401 agggtaaaag ttgctggatt cttggcgcgc tctgctgcaa atgctgtggt taatagcgct
    95461 gttccagcat tagtagcggt tccttcaata gcgccatgta gatcgcttag ttgaagtaac
    95521 tgaactgtct gccatgaagt tgaagtaacg ctagctagct cggcagcagt aaatgttttg
    95581 ctccaagttc ctgctggcga gaatccatag gcatttactg ccttaataga aactgttgcg
    95641 ctgctctgtc cagtgataat tggaagtgtt accactcctg aagttgttgc agggagcgaa
    95701 attgcgcatg atcctgcgcc acttgaaaga acataaccag tgactgcagg tgaggctgaa
    95761 actttttcaa agacaactct tatgccattt gaggctaagt atgctttttt gattactgga
    95821 gcagcaggaa ctgaaaaggt atttgcagta acttgcgggg aagtaagcac cactaatgca
    95881 gatgttgcta gcgctactgc gctggcaatt gcggagagtc gcagacggag ggaggggccc
    95941 tgggtctttg ggttttgtgt atgtgtgatc acaggcggat actggcatgg ccctggttac
    96001 aagttggtta atggcgcgtg agtgcggcat gtcccttagg tgagccttta ggcaatttgc
    96061 tggcgtcaga ggctctggct agtattcgaa caaatgttcg aaaagatgga gaatcagaga
    96121 ccggagatct acct
//
LOCUS       DAOROD010000003        85331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_8_length_85331_cov_29.940781, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000003 DAOROD010000000
VERSION     DAOROD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 85331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85331
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_85331_cov_29.940781"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLW53_01355"
     CDS             <1..492
                     /locus_tag="PLW53_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRD36106.1"
                     /translation="EENYQGVGWQQAHSIMGLASGGAVGTGLGSSKQKWGSLPEVHTD
                     FIFAVIGEELGLLGTLATLGLFGILILGIFKVAINAQNSFDRYVCAGIGCWISVQVLL
                     NIGTVISVIPVVGVTLPLISYGGSSLIATFIALGFVLGVLRRDPQIASEIRQRKAART
                     ARG"
     gene            498..1580
                     /locus_tag="PLW53_01360"
     CDS             498..1580
                     /locus_tag="PLW53_01360"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRD36107.1"
                     /translation="MAQRVVFAGGGTAGHIEPALAVAEALLLKDSKLTCEFIGVRGGL
                     ESLLIPKRGYRIHYIPKASFPRKISPKIFLFPILLVGAIFKARSIIKGSDLVIGFGGY
                     VSTPAYLAAFSRKIPILIHEANAKPGLANRLGRRLAKVTAVNFEKVAKQWSGSVLTGM
                     PIRQSLNSLAELPNKSSFKQLNCQSWGFDANRPVVAIFGGSLGSTHINSVIADFLKRY
                     QDKYQEVQIIHALGINNELPKASSNYLPQPYFHDMASIYGSADLLITRSGAVTCSELS
                     SLGKYSILIPLPHGNGEQFDNAAALVAQGSAMMVSNDDFSAEWLDKNLAAALTSAEKY
                     QAKSVGSNAKAAMRIAELAQEILLGK"
     gene            1587..2984
                     /gene="murC"
                     /locus_tag="PLW53_01365"
     CDS             1587..2984
                     /gene="murC"
                     /locus_tag="PLW53_01365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRD36108.1"
                     /translation="MSNQVIVEWSKARVHFVGIGGSGMSGIARIMASRGAEVSGSDLH
                     DSSTLNGLRSLGATIHIGHSRENIKSADLVIKSSAIPASNPELEEAESRGIPILERAA
                     ALAQLMIGTRSVAVAGTHGKTTTTSMLTVALQHSGLDPSFAIGATVSNSGTNAHQGSG
                     NTFIVEADESDGSFTAYKPLGAIITNIELDHVDNFATLAQIDQIFDDFVATIRPDGFL
                     VLCIDDPGALRLMERVKKKGSSISISTYGIDSDADLRLDRIHLQPKSAEARVSYKGRV
                     LGQMELAITGRHNLLNACAALLAGLELGANANDLIKGLALFSGARRRFEIKGISKGIT
                     VIDDYGHHPTEIKATLESARIYAQSGRVLTVFQPHRYSRTQVFASEFAKALSASDYTY
                     LLEIYPASEKEIPGVSSLLISKEMASSNHSYQPSMPEVVEAVAKMAKSGDLILTLGAG
                     DVSSLGKLILEAIES"
     gene            2989..3756
                     /locus_tag="PLW53_01370"
     CDS             2989..3756
                     /locus_tag="PLW53_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36109.1"
                     /translation="MKLNPRLILPISILLIGALAYLLGFSQVFVVKEIKIDIKDKSIA
                     REVEDRLSQPPAVVKLGDQIARVDRREIANRLRQFAWVENVELDRNLINGKLLIKIIS
                     RTPIARLTSTTSPQSVSIGFMGEDLDYFYLPRQAIDQAISSGDKSWQQIPELTLPAGS
                     SDGADNQLKAAARELIELFNQELAPRGFELTRINARDNASFITSANYSGRNIEISWGN
                     VNDLGLKVEVLERLLAQDENKKVRKINLIDPLKPTVR"
     gene            3886..5013
                     /gene="ftsZ"
                     /locus_tag="PLW53_01375"
     CDS             3886..5013
                     /gene="ftsZ"
                     /locus_tag="PLW53_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRD36110.1"
                     /translation="MATPQNYLAVIKVVGIGGGGVNAVNRMIDAGLKGVEFIAINTDA
                     QALLMSDADVKLDIGRKLTRGLGAGAAPEIGKQAAIDHTEEIEEVLRGSDMVFVTAGE
                     GGGTGTGGAPIIAKIAKDAGALTVGVVTRPFSFEGKRRAMQADEGIENLRKEVDTLIV
                     IPNDRLLSISDRSISAPEAFKTADQVLLSGVQGITDLITTPGLINLDFADVKSVMDGA
                     GSALMGIGSARGEARATRAAELAISSPLLEASIDGAHGVLLSIAGGSDLGLFEISEAA
                     ELVAKCAHPDANIIFGTVIDDALGDEVRVTVIAAGFDGGAPKRLSVPVISTAALNGQA
                     DPIAANDPIAVAMDTPVTPRRKITFEELVAEDEIDIPDFMK"
     gene            5025..5708
                     /gene="pgeF"
                     /locus_tag="PLW53_01380"
     CDS             5025..5708
                     /gene="pgeF"
                     /locus_tag="PLW53_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRD36111.1"
                     /translation="MSRSLFTSRHGGLSSNEFESFNLALHVGDDAKTVESNRDLLAKE
                     LGIKRSQLFFMNQVHGSRVVEIDENSTYLDEPEADALFTRRSGIALAVLTADCIPLLL
                     SSPSAIAAVHIGRKGLIAGVLEATLDKFESYGITSSQISALLGASICGTCYQVSLEIY
                     RQVVEQIEQCATDENKRCLDLEAGVISILKSRGITWSSRGECSKHSPGYFSFRRDNKT
                     GRQAGVIIR"
     gene            5705..6385
                     /locus_tag="PLW53_01385"
     CDS             5705..6385
                     /locus_tag="PLW53_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRD36112.1"
                     /translation="MSARQIEIKSALDNIRSQLKPGVELVVVTKNFPISDLEILYTLG
                     ERQFGENRENELSPKAEALPKDIDWHFQGTIQSNKLRAIVSVASYIHSLDELRHASKI
                     SELALEMGKKQSCFIQVNLDSEELVKQESNRSGIRAEQFKAFSESLLKLAGVEIVGVM
                     GVAPLNKDPRPGFETLRNLSQELLKVAPNAGFISSGMSGDYQIAMEYGATHIRLGSSI
                     LGLRRVGE"
     gene            6395..6844
                     /locus_tag="PLW53_01390"
     CDS             6395..6844
                     /locus_tag="PLW53_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRD36113.1"
                     /translation="MSNALRKMTNYLGLTEDDGTSEVATPKNRVAPVKRENKSLTVAP
                     SMTSAAKSVDAAPIDRIVTITPRFYSEARAIGEYYREGNPVIMNLSDMEESERKRLID
                     FASGLVFGHAGTIERVTSKVFLLSPPNVMVSSEDKTAAANASLFNQS"
     gene            6857..7135
                     /locus_tag="PLW53_01395"
     CDS             6857..7135
                     /locus_tag="PLW53_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRD36114.1"
                     /translation="MINISSWIVLALNLFLFALFARLILDYVRMFRPGWRPRGIILPV
                     AEIIYMITDKPLGFIRRFIPPLRMGPVALDLSFIVLFFGTQILIAFIR"
     gene            7195..8460
                     /locus_tag="PLW53_01400"
     CDS             7195..8460
                     /locus_tag="PLW53_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD36115.1"
                     /translation="MERSQAQALDAQDELGKFRNQFVITDPNTCYLDGNSLGRLPHAT
                     VKAISDFLTQEWGPKVVTGWEHWIDEAQTVGDLIGRSALGAGPGQVLACDTTSVNLYQ
                     LALAAIKARPNRKTIIIDAANFPTDRYIMQGIAKSLGLNLITINNEDSAISENELITP
                     ELLKSYLSDDVALVSLQVVQYRSGARQDIKTLTDLVKSHGALMLWDAAHAVGSVVLDF
                     DRNGVDLAVGCTYKYGNSGPGAPAWLYVSKGIQKELQVPIQGWFAQRDQFAMGANFER
                     VESIRGFQIASPSLMGLRCIKSAFAMIEEAGITAIEAKAASGTQMMIELYDEWLAPLG
                     FTLNTPKTASKRGGHISLCHPEASKIAIALRRYANVIPDYRAPNSIRVAISPLVNSYV
                     EVYDGFERIRDLVSTRQYEKIDEGQVRVT"
     gene            8457..9251
                     /locus_tag="PLW53_01405"
     CDS             8457..9251
                     /locus_tag="PLW53_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRD36116.1"
                     /translation="MSENHDAALTYSSYLKVDELLKLQQPLSEGPEHDELLFIVIHQT
                     YELWFKELIHEFLAAQRSLESGDTHYSLSILGRIRTIMKICVAQIDILETMTPLQFNS
                     FRDRLGASSGFQSAQFRQIEAILGRRDPKAAAHLLPADKKFVEDLMSSPSLWDSALRY
                     VDSRGFKIATEILNRDFSLAYQANEKVQEALLDLHRKDPEGSLICERLVDIDEGLQEW
                     RYRHVKMVERTIGHKSGTGGSSGATYLASTLFNPVFPDLWQIRSKF"
     gene            complement(9248..12394)
                     /gene="ileS"
                     /locus_tag="PLW53_01410"
     CDS             complement(9248..12394)
                     /gene="ileS"
                     /locus_tag="PLW53_01410"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRD36117.1"
                     /translation="MSSNSPYLALSASIDLPKVEHEILDFWQTQDIFHSSVRMRQGGP
                     SWTFYEGPPTANGLPGTHHIEARVFKDVFPRFQTMKGKYVIRKAGWDCHGLPVEIAVE
                     KELGFSGKGDIEKFGIAAFNEKCRQSVQRHVGAFTEMTERMGFWVDFDQAYWTMSNEY
                     IESVWWSLAQIHSKGLLVQDYRVAPYCPRCGTGLSDHELAQGYESVTDPSVYLRFPII
                     DGELKNLGASLLVWTTTPWTLVSNTAIAVNPDVDYLVVRTKADPEKEVISEVLVVAKN
                     LVEILGSSYEILRELKGSQLEKVKYQPPFNYVDIPDAHYVVLADYVTTEDGTGLVHQA
                     PAFGADDLQTCRKYGLPVVNPIAPNGEFESGIATVGGMFFKQADKILIKELKKSGSLY
                     KHQQYEHSYPHCWRCHTPLIYYAQPSWYIRTTKIKDALLRENASTNWHPETIKNGRYG
                     DWLNNNIDWALSRNRYWGTPLPIWRCENKHEEAFASKAQLQGRYGKDLTDLELHRPFV
                     DEIVFNCLTCSSQMVRTPEVIDCWYDSGAMPFAQWGYPHKQGSEEKFKEAYPADFICE
                     AIDQTRGWFYTLMAIGTLVFDKSSYKTVLCLGHILDKDGRKMSKHLGNVLEPMALMDK
                     HGADAVRWYMLAAGSPWSARRVGHDAISEVVRKTLLTYWNTVSFLTLYASAANYSPTP
                     ITKASNLSTMDRWILSELNQLIATVDQALSDFDSQLAGSAIATFIDDLSNWYVRRSRR
                     RFWDGDSAALSTLHHCLKNVTLLMAPMVPFITEHVWQKLIRVVEKDGPISVHLADFPV
                     SEASLIDKELSKSVALTRRLVELGRASRAQSGVKIRQPLSRALIAAPGWKLLSVELRE
                     QISDELNVESLDDLASAGADLVDISIKANFRSLGAKFGGEVQELSKAITSSNAQELVS
                     KVRNSGKAQITYGSKTAEITEEDLVITETPRTGWAVASHTGESVALDLNLTDELIAKG
                     VVREAIRAIQDERKASGFDVSDRIHVKWNSDEQSSKAIEAGAAWISEEVLALSFERDS
                     KLTPSEQELGISLKLTKA"
     gene            12660..13475
                     /locus_tag="PLW53_01415"
     CDS             12660..13475
                     /locus_tag="PLW53_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRD36118.1"
                     /translation="MALKKKVRSAVAKFKKKSSPAKAKPKAKSVKPKAKKAPVKKVTA
                     KKAPAKKVPVKKAPVRPTLAKKAPLKKAPAQIMKGAHKTALTSSTAKGGATTISVYKP
                     EIEASDLVVVPEKRLVMPPPIRTPKPGKKAPALKPVKAAPSPFVLDGEESWSAAEMKA
                     IKSELSKDLIRLKKELETIESEMDDLIEASGVGAGDDQADAGAKTFEREHEISLVYNA
                     RDMVLQTERALERIETKTYGRCEDCGSAIGKARLQVFPRATLCMLCKQKEERR"
     gene            13448..13966
                     /gene="lspA"
                     /locus_tag="PLW53_01420"
     CDS             13448..13966
                     /gene="lspA"
                     /locus_tag="PLW53_01420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRD36119.1"
                     /translation="MQTEGRAPIRSSGANRARRLFVIFTVSIFALDLITKNWAISYLQ
                     YREPVKVIGDFLQLTFATNPGAAFNLIGDATLILSSLKLCVAALILYFIRKVTNGSWA
                     IALGLLLGGVLGNLMDRAFRPPGPWRGEVIDWIELPNWPVFNIADSAIVTSALFMSLL
                     AIRNIEPYKKVI"
     gene            13966..14895
                     /locus_tag="PLW53_01425"
     CDS             13966..14895
                     /locus_tag="PLW53_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRD36120.1"
                     /translation="MEREKRAIQIDQDQDGQRVDTALAKVLELSRSVVADLLNAGDVL
                     QGKKPLSKSDRVSAGDRLTVLMPAIYDPLELKETPIDTLEIIYDDDDVVVVNKPVGCA
                     AHASPGWMGPTVVGALLARGYRISTTGPQERQGIVQRLDAGTSGLMLLAKHERSYISM
                     KNQFRNRSIEKVYRTLIQGHIDPAEGSIDAPIGRHPREDYRFAVVADGKASITHYELI
                     EYYQGASLLKVVLETGRTHQIRVHFNALRHPLVGDLAYGGDPVLAARLELKRPWLHAL
                     ELSFNQPSSDKRITLNAPLPEDLTRALALLAVK"
     gene            14921..18442
                     /gene="dnaE"
                     /locus_tag="PLW53_01430"
     CDS             14921..18442
                     /gene="dnaE"
                     /locus_tag="PLW53_01430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRD36121.1"
                     /translation="MSQGFVHLHVHTEYSMLDGAARIEKLAEEVARSGDGAIAMTDHG
                     NVFGAFDFYKTMNNAGVKPIIGIEAYVAPESRFDKKRVKWAEGGEDDVSAGGAYTHMT
                     LLAENNVGLANLFRLSSLASLEGFYYKPRMDRELLSQYSQGIIATTGCAAGEIQTRIR
                     MGAYNEARAAAANYRDIFGADNYFVEIMDHDIEIERRVKDDLLKLAKELGLPLVATND
                     LHYTYADDAKHHEALLCIQSGTTLADPKRFRFEGKEYYLKDAATMRRIFKDLEIACDN
                     TLAIAERCNIKMRENENLLPQFAVPKGESENSWLVKEAEAGLLEKFGAKSMDDIAPQY
                     QARLHFELDVMTKMGFAGYFLVVADLVAHAKREGIRVGPGRGSAAGSLVSYALGITGL
                     DPIKHGLLFERFLNPDRISMPDIDLDFDERRRSEMIRYATNKYGDDRVAQIITYSTIK
                     SKAAIKDAARVLGYPYALGERLTKALPPPIMGKDVSFHGVFDKDDERYGEAQEFRNIY
                     ESDPDSKTIVDMARGLEGLKRQWSVHAAGVILSREPLIDVIPIHKRDVDGSIITQFDM
                     GACEATGLLKMDFLGLRNLSVLDDCLENIKNNQAKTIVLESLELSDKKTFELLSRGET
                     LGVFQLDSAPIRALLRSMAPDSFEDISAVIALYRPGPMGVNAHNDYADRKNKRKRIEP
                     IHPELSAALDEILADTYGLIVYQEQVMAIAQKLAGFSLGRADLLRKAMGKKNKEILDK
                     EYVHFEEGMRNNGFSSIAIEELWKTLIPFSDYAFNRAHSAGYGVLSFWTAYLKANYPT
                     EYMAALLTSVRDDKDKSALYLNESRRMGIKVLPPDVNFSNAEYTPGGSDIRFGLTAIR
                     NIGENVVASIVKNRNEKGNYTSFSDFLAKVDGTVCNKKTIESLIKAGAFDSLGHSRKG
                     LMSIYLEAIDSVMEEKRAQAIGQFDLFGGANEVSANGISTLALEVSDQEWDKALLLSY
                     EREMLGLYVSDHPLLGVEHVLRSATDTTISQILDEGVVHDQIITIAGLITAVQRKVSR
                     QGQSWAIVTVEDLEGAIDVRFFSSTYQTHALNLIEDRIVIIRGRIDKREETAQITALE
                     LNLADINQAPIGPFVIKLEAERCTPPVVDRMKEILRSHPGTREVHLKIEGGAKNTILK
                     LDDGLKITASPSLSADLKSILGPDCLV"
     gene            18494..19219
                     /gene="priA"
                     /locus_tag="PLW53_01435"
     CDS             18494..19219
                     /gene="priA"
                     /locus_tag="PLW53_01435"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HRD36122.1"
                     /translation="MEKLELLPALDIRAGEAVRLIQGDLSQQAKYGSALDVALEFQSA
                     GAEWIHLVDLDAAFRTGSNYDLLVEVVGKLDVKVQLSGGIRDQESLERALATGCHRVN
                     LSTSALLDLSWCESVIKRFGEKVAVGLDVTGRTLAARGDSQEVGDLFDVLKQLDGYGS
                     SRYVLTDVSRDGTLEGPNLDLIKSVCAETDRPVIASGGISQLSDIQSLRELIAIGVEG
                     AIIGKALYAQNFTIGQALEVAAK"
     gene            19273..20778
                     /locus_tag="PLW53_01440"
     CDS             19273..20778
                     /locus_tag="PLW53_01440"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRD36123.1"
                     /translation="MERMNLEQFRSYAKEYNVIPVSQVLLADNETPLTLYSKLVQNRE
                     NTFLLESAEHGGAWSRYSFIGVRSEATLTEKNGVAHWLGSMPAGAPNGIDPLEALMIA
                     SKHLRSPKIENLPPLTGGLVGYMGYDAVRRLEKLPNLGIDELKLPEIAFMLTTDLAVY
                     DHLQGTVILIANAINWDGSDERVDLAYQSAINRLDQMQEALLNKIDSNISKIAKKSAP
                     SFTANFTKDQFCKSVESIKEQIRSGEAFQVVLSQRFTMDCQASSLDAYRMLRLHNPSP
                     YMYHFRFADGLSVVGSSPEALVKVTGSEVMVHPIAGTRKRSTNQEEDLRLGEELLADP
                     KERAEHLMLVDLGRNDLGRVCAPGSVNVVEFMQIERYSHVMHIVSTVIGELDSAKSPV
                     EALYAVFPAGTLSGAPKPRAMEIIEELEPSRRGIYGGAIGYIDFTGNLDTCIAIRTAV
                     IKDKKAYVQAGAGVVADSNPVSEYEECLNKAAAVLGAIEAANSLEPLGEKD"
     gene            20784..21563
                     /gene="trpC"
                     /locus_tag="PLW53_01445"
     CDS             20784..21563
                     /gene="trpC"
                     /locus_tag="PLW53_01445"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRD36124.1"
                     /translation="MSVLNSIIAGVLEDQARRQLSNSELEEKMALAAKPLDVLAALSR
                     EKFSIIAEVKRSSPSKGSLAEIPSPEKLARHYQEGGAAVISVLTEQRRFGGSLEDFEK
                     VRSEVTIPLLRKDFIVNEYLVKESRAYGADLILLIVAALGDDELKQLYQLVKSLGMEA
                     LIEIHNQEELERALAIGPKIIGVNARNLKTLEIDQSNFAKLLPLIPSGIVRIAESGMS
                     EPSDGQSALIAGADAILVGEALVKSENPADSVREFLNVANS"
     gene            21584..22774
                     /gene="trpB"
                     /locus_tag="PLW53_01450"
     CDS             21584..22774
                     /gene="trpB"
                     /locus_tag="PLW53_01450"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRD36125.1"
                     /translation="MERVEVVGHFGPYGGRFVPEALIGALDELEAAHNSASKDPEFQR
                     ELNHLHKSYTGRPSIITEAKRFAEHAGNARILLKREDLNHTGSHKINNVLGQALLTKR
                     MGKKRIIAETGAGQHGVASATAAALLGLECVVYMGEEDTKRQSLNVARMKLLGATVIP
                     VTTGSRTLKDAINEAMRDWVTNVSTTHYLLGTVAGPHPFPTMVRDFHRIIGIEARQQV
                     LEMTGRLPDAVLACVGGGSNAIGIFHPFIDDAGVRLIGLEAGGAGVESGKHAATIVGG
                     TPGVLHGTRSYLLQDSDGQTIESHSISAGLDYPGVGPEHAYLHDIGRAEYRAITDDQA
                     MHAFSLLSKSEGIIPAIETAHALAGALQVGNELGSGAILLINLSGRGDKDVQTAAQYF
                     GIPL"
     gene            22789..23592
                     /gene="trpA"
                     /locus_tag="PLW53_01455"
     CDS             22789..23592
                     /gene="trpA"
                     /locus_tag="PLW53_01455"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRD36126.1"
                     /translation="MSKESLAQVFARAKSENRAALIGYMPAGFPTLEGSKELIRALIA
                     GGVDIIEVGFAYSDPVMDGAIIQEAAEIALRNKTGAKEVLEVVSGVSVPSLVMSYWNP
                     IERFGVKEFTKALASAGGVGVITPDLTIEESGDWIVETERNDLQRVYVVAPSTDDSRL
                     AKVADKCSGFVYAASLMGVTGARNSLSDSASELVARLRKVTNLPIAVGLGVSTAEQAA
                     DVAKFADGVIVGSAFIKLVLQAPDFTQACKNVTLLARELSDGVRQGRVG"
     gene            23658..24404
                     /locus_tag="PLW53_01460"
     CDS             23658..24404
                     /locus_tag="PLW53_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD36127.1"
                     /translation="MPKEPRQRKVSNSAKSGDTVTRNIVIGMIALVVLSGVIFTVLDK
                     RSGSSVALPASIESISSANNGEPLTPTISPEADYGVVFNADSPLKIDIWEDFQCPYCK
                     FFEDAMGDYLETLIREKQAEVTYHMASFLGQESVRASNAANCAVPEGRFIEYHRALFD
                     IQGGENSGLFSNKNLVEIGTRLGITSESFANCVNNNESGDVVKNVASSMKKNGVEGTP
                     TVFINGKPWNRTTSEFRLEEFKAAVEAAKQ"
     gene            24401..25222
                     /gene="lgt"
                     /locus_tag="PLW53_01465"
     CDS             24401..25222
                     /gene="lgt"
                     /locus_tag="PLW53_01465"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRD36128.1"
                     /translation="MIFAAIPSPTQSQIEIGPLTFHFYALSIITGIIAAVFIGNRRYV
                     ALGGRAGVVSDVAIYAVPFGIIGGRLYHVISSPQAYFGTNGDPLSALYIWQGGLGIWG
                     AISLGLLGAYIGYRRNKSRGDVSFASFADALAPGLLIAQGIGRWGNWFNKELFGRELN
                     APWALEIPASYRPIGYSSVETFHPVFLYESIWCFIAALILIKVGSRKRFAPGSIFTLY
                     VALYCLGRALIETIRIDEANLIFGVRLNVWTSGVLLFASLIYLRQNQLPKESSVK"
     gene            25227..25934
                     /locus_tag="PLW53_01470"
     CDS             25227..25934
                     /locus_tag="PLW53_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HRD36129.1"
                     /translation="MSQSDLEIRNSEHRTHRSGWLRAAVLGVNDGLVSTAALMIGIVS
                     AQQNDFVVTVGMAGIAAGAMSMAVGEYVSVRSQNDIEESDRILELEHHRIDPAGELEE
                     LTQIYQSRGLSRATAEKVAQELHAHDALGAHLRDELGQYELTKARPMQAAIASALSFT
                     LGGAIPLIGALAPSLGGKAVSIVAITFFGLLIAGVISAKTSGSKIWRPTLRILLGGAL
                     GMAITASVGQIAHISGL"
     gene            26058..30575
                     /gene="gltB"
                     /locus_tag="PLW53_01475"
     CDS             26058..30575
                     /gene="gltB"
                     /locus_tag="PLW53_01475"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRD36130.1"
                     /translation="MVFSSLPKAVGLYDPANEHDACGVAMVATLNKVATHEIIAKGLV
                     ALRNLEHRGASGAEVNSGDGAGILICIPDQFFRAVLPFELPAPLRYAAGVVFLPKNFA
                     SKSRIEQIASEENLIILGWRQLPINASSLGATALSVMPEFEQIFVSSQDGVSDLDLDR
                     RAFCFRKRVEHELSIYFTSLSSRTIVYKGMLTTGQLEEFFPDLSDPRLVSPLALVHSR
                     FSTNTFPSWPLAHPYRFIAHNGEINTVRGNRNWMAAREALLESDLIPGDISRLFPIVD
                     PSGSDSASFDEVLELLYLGGRSLAHSILMMIPEAWENHETMPEKLKDFYRFHASLMEP
                     WDGPACVTFTDGKQIGAVLDRNGLRPSRYWVTDDGLVVLASEVGVLDIPQEKVIRKGR
                     LQPGRMFLVDIQSGRIIEDEEIKQSLATNAPYGEWLHAGIVRLSDLPAREHIIYPHSS
                     VLRRQRAFGYTEEELRMIVTPMAKSGIEPIGSMGTDTPIAALSAKPRLLFDYFSQLFA
                     QVTNPPLDAIREELVTSLGGSIGPEHNLLDPGPASCRQISLAFPVIDNDELAKIINVN
                     ADNNHPGLSTYVIRGLFPISKGGQGLQERIEEIRAEVSEAISQGARIIVLSDRDGDAE
                     NAPIPSLLLTSAVHHHLIREKTRTKVGLVVESGEVREVHHVALLIGYGAAAVNPYLAM
                     ESAEDLVLTGVITGITPEKAVKNLIKSLGKGVLKVMSKMGISTIASYTGAQVFEAIGL
                     SQALVNEYFVGTTSRLGGVDLEVIAQETIARHHIAYPPGGEVPGSKRLPIGGEYQWRR
                     DGEPHLFDPETVFALQHATRSKRYDIFQRYTKRVDDQSKSLMTLRGLFTFKQGLRKPV
                     AIDEVEPVSEIVKRFSTGAMSYGSISQEAHETLAIAMNRIGAKSNTGEGGEDPERFIP
                     QANGDSARSAIKQVASGRFGVTSEYLVNADDIQIKMAQGAKPGEGGQLPGNKIYPWIA
                     KVRYSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANNRARIHVKLVAEVGVGTVAAG
                     VSKAHADVVLISGHDGGTGASPLTSLKHAGAPWELGLAETQQTLMLNGLRDRIVVQTD
                     GQMKTGRDVVIAALLGAEEFGFATAPLVVSGCVMMRVCHLDTCPVGVATQNPELRKRF
                     TGKPEFVETFFEYIAQEVREILASLGFRSLSEAVGAVESLETSAAVEHWKASGLDLSP
                     ILAAPESASGSARRNTTTQDHGLEAALDNELIKLSQSALESKSPIKLQMKIRNGNRTV
                     GTMLGSEITRKYGGQGLADDTIDITFHGSAGQSLGAFIPRGLTLRLYGDTNDYLGKGL
                     SGGRVILRPDEKAKFASHENVIAGNVIGYGATQGEIFVRGIVGERFCVRNSGALAVVE
                     GVGDHGCEYMTGGTVVILGKTGRNFAAGMSGGRAFVLDLIPSLVNTEMVDVLSIPKDQ
                     EEKLKSILSRFYAETGSLIAHALLGDWQAALKRFSLVMPRDYAKVLAVIDRAEREGKS
                     SDDAIMEALNG"
     gene            30568..32025
                     /locus_tag="PLW53_01480"
     CDS             30568..32025
                     /locus_tag="PLW53_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRD36131.1"
                     /translation="MADPRGFLNTGREVPKRRPVDVRIRDWREVYEEQEFSSLQKQAG
                     RCMDCGIPFCHQGCPLGNLIPEWNDLIWRNEKVDALDRLHATNNFPEFTGRLCPAPCE
                     TACVLGINQDPVTIKQVELRIIEEGFQSGNVKPLQPDRMTGKTIAVIGSGPAGLAAAQ
                     QLTRAGHTVVVYEKGEKVGGLLRYGIPEFKMEKAILDRRINQMQREGTRFRAGVNVGV
                     DISAAELRAKYDAIVLAVGATQWRELDIPGRSLNGVYQAMEYLPWGNKQALNELPSEP
                     PINAKGKNVIILGGGDTGADCLGTAIRQGAASVTQIEIMPRPSDERPDSQPWPTYPMT
                     YRVSSAHEESGERLYSISTEEFIGNSAGQLTALKIVETKYVDGKFEKVPGTSRDLPAD
                     MVLLAMGFTGPEKNELVKALELKLDSRGNIARDNSYRTNVDSVYICGDAGRGQSLIVW
                     AIAEGRSAASAVDADLMGHTQLPSPIEPTARPLTV"
     gene            32073..33512
                     /gene="pyk"
                     /locus_tag="PLW53_01485"
     CDS             32073..33512
                     /gene="pyk"
                     /locus_tag="PLW53_01485"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRD36132.1"
                     /translation="MRRAKIVCTMGPAVESVEKVSELIRAGMNVARLNLSHGGYEEHQ
                     NRLDLVRAEAARVKQPVAIMVDLQGPKIRLGRFENGPHELMRGDIFTITTDDITGDKS
                     RVSTTYKGLPGDCKAGDLILIDDGKVTVQVIEVKGSDVITKVIEPGFVSNNKGINLPG
                     VAVSVPAMSEKDIEDLRWGLKAGADFIALSFVRSAKDIDDVYKVMDEVGHRIPVIAKI
                     EKPQAVDNLEEIVLAFDGIMVARGDLGVEMPIEDIPLVQKRCITLARENAKPVIVATQ
                     MLDSMINNSRPTRAEATDCANAVLDGADALMLSGETSVGAFAIEAVSTMARIIEKTEK
                     EALHLIPALQHNPKTKGGAITKAATEVGLTIGAQVLAAFTKSGDSARRMSRLRSPIPI
                     LALTPDTATYNQMALTWGVEPLLTPLVTTTDQMVKQVDTILIESKRVAVGELIMIVAG
                     SPPGIPGSTNAMRVHKVGDAVSGVAPAYR"
     gene            33534..33606
                     /locus_tag="PLW53_01490"
     tRNA            33534..33606
                     /locus_tag="PLW53_01490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33567..33569,aa:Leu,seq:caa)
     gene            33608..34204
                     /locus_tag="PLW53_01495"
     CDS             33608..34204
                     /locus_tag="PLW53_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD36133.1"
                     /translation="MSINIKSGSHRILIAEDETLIRMDLVEMLSESGYEVVGQASNGQ
                     EAIDLAKELKPDLAILDVKMPILDGISAATQIIEISPVLMLTAFSQKDLIERASEAGA
                     MAYVVKPFTINDLIPAIEISISRHRQMKSLALEVSDLHERLESRKLIDRAKGILMKAL
                     NLSEPEAFSWIQRAAMDRRISMKEVAKAVIDPSAAPNK"
     gene            34343..35497
                     /locus_tag="PLW53_01500"
     CDS             34343..35497
                     /locus_tag="PLW53_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD36134.1"
                     /translation="MKKRGLISVAAAAALAAASLVAVAPASNAAAKTITLAFQGPLTG
                     EEAQVGQDELAGMLYAVKLFNAKNKGKYVVKVVQADDQGDPAQSAKVAPGIASNKKII
                     GLVGAAYSGATIAALPYYKQTLLPMISPSATRVSITNPAAEGGAMGVPVFHRVPPTDK
                     VQGPSLFKLATKGVSSPKVFVVDDQSAYGVGLVDYMKPAIPAGQSAGFDSVSDKTTDW
                     SATISKIKTAGANVVIYTGYFPQAAPFFKQLRDSGYTGILAGGDGVLSPSFVTLAPAS
                     VLEGVRLTAGTVPLSEISAKLEADFKKSMGKASGVYAAESIDATNVFLSCVAKGVSTR
                     DAMLKCVKAYSGKSLTGATIKFDAYGDVAGGVMNGFEVRGGAIKFTGPVK"
     gene            35621..36538
                     /locus_tag="PLW53_01505"
     CDS             35621..36538
                     /locus_tag="PLW53_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD36135.1"
                     /translation="MTFGGLSLGFIYILIALGYTMVYGVLRMINFANSEIFMVGTFAT
                     IIIIRDVFKFTQASEPATGARLWLILATALIFSMLVSGVLSMLVEIVAYRRLRRMNSG
                     VLAPLISAIGVSMVLAESVRILTDSRPVASPRAMEKKFIGQFFDAGVRIDNTIIVIAA
                     LILFAVLITFVNRTRMGKAIRAVSMNPNAARLMGINIDGVVSATFLVGGLMTGAAGFF
                     YVLKYENTASNIGFELGIAAFTAAILGGIGNIKGAFYGGLALGLLETYASFFLGTQWK
                     AVTVFIVLVLILYTRPNGLFGEALTQTRA"
     gene            36535..37557
                     /locus_tag="PLW53_01510"
     CDS             36535..37557
                     /locus_tag="PLW53_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD36136.1"
                     /translation="MMINLRDKGAEIWERQGRLGRVGIALGGVAIAAALPFMGGTILN
                     TPIADYESVLVYPIAMYVLMAIGLNIVIGKSGLLDLGYVAFFAIGAYTMGLLGTKTDL
                     NTWEILPIGIAMAMFSGIILGVPALKLRGDYLAIITLGFGEIVRIVALNLGALGRSEG
                     IINIPTPPTIFGIEYRFDSHRIYYWTLLVLILFAIWMVRRLSVRRAGRAWESIRQDED
                     AAELLGVPTFKYKVWAFVLGASVGGAAGVFYASKTLFIAPDNFTLQISILILACVVFG
                     GMGNIWGVILGAVILGYLPDRIRFISDARLLVFGLVLVIMMNLRPDGLLPRKKREKAI
                     VRKDAS"
     gene            37554..38330
                     /locus_tag="PLW53_01515"
     CDS             37554..38330
                     /locus_tag="PLW53_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36137.1"
                     /translation="MTSSLIQLKNVTMKFGGVTALSDVTFDVKKQEILGLIGPNGAGK
                     TTVFNVITGVYQITSGEIIFDGKSLSGVKRYKITKRGVARTFQNIRLWGDMTALENVM
                     TATDTHKKSGLVSGLFGFPISRREEKRDKARAQELLDFIGIGEYADRLAKNLPYGVQR
                     RLEIARAMGAEPKLLLLDEPAAGFNPAEKVELAATIRKIRDAGYTVLLIEHDMSLVMK
                     VSDRVVVLDFGQKIADDSPRKVQDDPRVIEAYLGVPEDAS"
     gene            38320..39048
                     /locus_tag="PLW53_01520"
     CDS             38320..39048
                     /locus_tag="PLW53_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36138.1"
                     /translation="MRLEIKDLRVAYGKIEAVKGISIVVNQGEIVSLIGANGAGKTTL
                     LKTISGLLNPTSGSITYDGEDILKYKAHQRVSLGISQAPEGRGIFPGMTVLENLELGK
                     YHLKMKKSEIAEDLATVYELFPRLKERATQAGGTLSGGEQQMLAIGRALMAKPKVLLL
                     DEPSMGLAPQMIANIFRIITQINKIGVTILLVEQNAQQALQRSDRAYVLEVGNITKEA
                     KGKDLLNDPHVKSAYLGTGAHELD"
     gene            complement(39049..39453)
                     /locus_tag="PLW53_01525"
     CDS             complement(39049..39453)
                     /locus_tag="PLW53_01525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD36139.1"
                     /translation="MTDFSEILNDRGRGALDKKMGIEIIEASPQRLVGRMPVEGNTQP
                     FGLLHGGANVVLAESLGSVGTHLHAGPSRRIVGIEISASHHKSATEGYVTAVATAVTL
                     GKTLCTYNVEITNDKGEKTCTARITCLILAQR"
     gene            39493..42048
                     /gene="polA"
                     /locus_tag="PLW53_01530"
     CDS             39493..42048
                     /gene="polA"
                     /locus_tag="PLW53_01530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRD36140.1"
                     /translation="MKKKLLILDGHSMAFRAFYALPVENFSTSSGQPTNAIYGFASML
                     INLIRDEKPTHIATAFDVSRKTFRSEKFPEYKANRAATPEEFRSQVSYINELLDAFAI
                     PHFELEGYEADDVIATFVKHFSDEAEIYICSGDRDSFQLVSDQVTVLYPKKGVTELSR
                     MTPSAVFEKYGLTPRQYPDFAALRGDPSDNLPSIPGVGEKTATKWIQEFGSLSELIAK
                     VDTVPGKAGEALRTALPAVITNRELTQLRNDLPLSPDLTDLAWSGVEILKVNQLFEKL
                     EIKALKARVAPFAKGGQGKEVVAKKASVRNVSKSEFEKALSSSTGLVGLILSENQAAI
                     SIEPEVVLVAPLSEVSDSITSFDGFIFHGAKKVISSKLLRSVAVDTEVAAYLLNAGSR
                     DIALADLLQKYLGVESENTPTDLFSTDWNPSIASHLGELWATLSKELEAMSCLDLYNQ
                     IEMPTMHVLAEMEATGIAIDKAEMKSLLSFFEKEEAEATSLAYKSVGHEFNAASPKQL
                     QVVLFEELKLPKTKRIKTGFSTDAESLEWLYSTTKHPVLASLLRIREVGKLRTTVDGL
                     LNSTKGDSRIHTTFQQTTTATGRLSSTEPNLQNIPIRSDEGRRIRNCFIAQDPFVDLL
                     TADYSQIEMRIMAHLSDDKGLLEAFRTGEDLHSTVGAQVFDVPVSKVDADMRRQIKAM
                     SYGLAYGLSSYGLAQQLDISPTDASILMSKYFERFGGIQDYLKEVVKIAREKGYTETI
                     LGRRRYLPDLNHENRGRREMAERMALNAPIQGSAADIIKVAMLNVEKAMKSKNLSSRL
                     LLQVHDELIFEIAKGEHEVMEELVRKQMGSAFELKAPLDVNIGFGKSWDLAAH"
     gene            42217..43563
                     /gene="rpsA"
                     /locus_tag="PLW53_01535"
     CDS             42217..43563
                     /gene="rpsA"
                     /locus_tag="PLW53_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRD36141.1"
                     /translation="MSTQITVNDVGNAEDFLAAIEGTIKNFNDGDLVSGVIVQVDRDE
                     VLLDIGYKTEGVIPARELSIRHDLDPSELVKVGDRVEALVLQKEDKEGRLILSKKRAQ
                     YEKAWSDIEGKKERDEVVTGTVIEVVKGGLIVDIGLRGFLPASLVEMRRVRDLSPYIG
                     KEIEARIIELDKNRNNVVLSRRAFLEQSQSESRTTFLNQLIKGQVREGVVSSIVNFGA
                     FVDLGGVDGLVHVSELSWKHIDHPGEVVAVGDEVTVEVLEVDFERERVSLSLKATQED
                     PWQAFARTHTINQVVPGEVTKLVPFGAFIKVFDGIEGLVHISELAERHVEIPEQVVQV
                     GDSLFVKIIDIDLERRRISLSLKQANEGQDVAVEAFDPTQYGMPARYDENGNFIYPEG
                     FDASTQEWQPGYETQRQEWERQYAEAQTRFLAHKKQKAEAQAADAAAASVATPEAE"
     gene            43636..44241
                     /gene="coaE"
                     /locus_tag="PLW53_01540"
     CDS             43636..44241
                     /gene="coaE"
                     /locus_tag="PLW53_01540"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRD36142.1"
                     /translation="MLRVALTGGIGSGKSLVGEILEELGALVIDSDQLAREVIERGSP
                     GYEEVVTAFGDSILSEGQIDRSKLAAVVFKESDLRKKLESIIHPLVREAAEKLARKLP
                     SGAILINQIPLLVESDGAKRFDYVVTVSADEEIRRERLRLRGLKDYEITQRMAAQVSD
                     SEREKIANYIIRNDASIDELTRAAEALMANELLPRAQKQGL"
     gene            44243..46324
                     /gene="uvrB"
                     /locus_tag="PLW53_01545"
     CDS             44243..46324
                     /gene="uvrB"
                     /locus_tag="PLW53_01545"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRD36143.1"
                     /translation="MARAVTSTPRANRAFEVISDFKPAGDQPDAIAELTRRINSGESD
                     VVLLGATGTGKSATTAWLIEAVQRPTLVLAPNKTLAAQLANEFKELMPNNAIEYFVSY
                     YDYYQPEAYVPQTDTFIEKDSSVNSEVERLRYSATYSLLTRRDVIVVASVSCIYGLGT
                     PQEYIDRMITLAVGDKIDRNVLLRRFVDIQYKRNDMAFERGTFRVRGDTVEIIPVYEE
                     LATRIEFFGDEIERLTTLHPLTGEIVSDVKEVFIFPATHYSAGPETMKRAIANIEAEL
                     DVRLKELEKANKLLEAQRLRMRTTFDIEMMLQLGFCSGIENYSRHIDFREPGSAPNCL
                     LDYFPEDFLCVIDESHITVPQIGAMYEGDASRKRTLVEHGFRLPSALDNRPLKFAEFL
                     ERTPQKVYLSATPGPYEMEKTGGVFVEQVIRPTGLVDPKIVLKPIKNQIDDLMSEIKV
                     RAEKNERVLVTTLTKKMAEDLTDFFTDAGIRVRYLHSEVDTLRRVELLRELRLGEYDV
                     LIGINLLREGLDLPEVSLVAILDADKEGFLRSTRSLIQTIGRAARNVSGEVHMYADKI
                     TDSMTRAIDETNRRRAKQVAYNLAAGLDPQPLRKKIADITDLIAEESDDTNALLAGNK
                     KRGMSQTPVGVHAKSLVALPLAELLGLIDSLTEQMRNAAAELQFELAARLRDEIKELK
                     RELRGMQEGGM"
     gene            46324..49161
                     /gene="uvrA"
                     /locus_tag="PLW53_01550"
     CDS             46324..49161
                     /gene="uvrA"
                     /locus_tag="PLW53_01550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRD36144.1"
                     /translation="MSADRLIVRGAREHNLKNVSLDLPRNAMIVFTGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGT
                     ITEVYDYLRLLFARAGKPHCPKCGKEIAKQTPQNIVDQILELPAETKFQVLAPVIRAR
                     KGEFVDLFKDFIAQGYSRVRVDGTTVALTEAPKLKKQEKHTIEVVIDRLTAKPEGRQR
                     LTDSIETALKLANGLVTLDFVDAKGADKERTYSELLACHDCGLSFQEMEPRSFSFNSP
                     FGACAECTGIGSKLEVDEGLVIPDDSISINDGAIAPWSGGQSSDYFINLLEALAADVK
                     FSLNTPWSKLSVKAKDAILNGYEYEVHVKYKNRYGRVRNYSTGFEGVIPFVHRRHSET
                     DSDYSRERFEAYMRQVPCPACKGSRLKPEVLAVTLGSKNIAEICELSIEDCAKFLKGL
                     KLASREAKIAERVMKEVHARLGFLLDVGLEYLTLARPAATLSGGEAQRIRLATQIGSG
                     LVGVLYVLDEPSIGLHQRDNKRLIETLTHLRDLGNTLIVVEHDEETIRTADWIVDIGP
                     GAGEHGGEIVVSGDYQALIDSKKSITGAYISGRSKIDIPKKRRAINAKRVLTIKEAKE
                     NNLRNVDVTIPLGVFVAVTGVSGSGKSTLVNDILYSVLANKLNGARIVPGRHRSITGI
                     DQLDKVVHVDQSPIGRTPRSNPATYTGVFDKIRALFSETSEAKVRGYQQGRFSFNVKG
                     GRCENCSGDGTITIEMNFLPDVYVPCEVCHGARYNRETLEVHYKGKTIAEVLDMPIEK
                     AQDFFESIPAIHRYLKTLCDVGLGYVRLGQSAPTLSGGEAQRVKLSTELQRRSTGRTI
                     YVLDEPTTGLHFEDVRKLLGVLQRLVDTGNTVVVIEHNMDVIKCADWIIDLGPEGGSG
                     GGSIVAEGVPEAIAKVSASYTGKFLRETLKK"
     gene            49164..51077
                     /gene="uvrC"
                     /locus_tag="PLW53_01555"
     CDS             49164..51077
                     /gene="uvrC"
                     /locus_tag="PLW53_01555"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRD36145.1"
                     /translation="MADPASYRPENVPDEPGVYRFFNEKDEVIYVGKARSLKNRINSY
                     FQKNVGEKTYRMIHSANRVDWTLVRSEVEALQLEFTWIKEENPAFNVQFKDDKSYPYL
                     ALTMDERYPRLLITRRAKQKGVTYFGPFTHAWALRSTFDVLLKLFPVRSCSNSNFERA
                     QRAKRPCLLGDIGKCAAPCVDRINESDHRILAQRLGEFISDGSADITQALRDQMAEAA
                     RAEEFERAGKLRDQLEALERASESTDSALSDSIFSDFIAIHQDITHAAISIFRVRAGR
                     VISSRSWMIDLANLPEDSSLLDACINRVYQDFEIGKEILVNQEIDGDALAEYLSERAG
                     YKVTIKKPERGEKSEILDMVKRNANQALIQFLSKRANDAGVSGRALEDLASALNLEEL
                     PLRIECFDISNIQGTSVVASMVVFEDGQAKKSEYRRFGIDDKEKFDDTRAMHHVITRR
                     FKRYLAEKDLDLSEIELSGGPRPKFAYPPQLVIVDGGKGQVNAAARAFAELGITDIAL
                     VGLAKRLEEIWFADQSYPVILDRHSEALYLVQRIRDEAHRFAVTFHRSKRSKLMLESL
                     LDEIDNLGEVRRAVLLEHFGSIAALSKASLEEIAALPGIGPKSAESILAGLANSASGY
                     FVDTSTGEIIERS"
     gene            51099..51971
                     /gene="rapZ"
                     /locus_tag="PLW53_01560"
     CDS             51099..51971
                     /gene="rapZ"
                     /locus_tag="PLW53_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRD36146.1"
                     /translation="MGREIVVITGMSGAGRSTVAHAMEDHGWYVIDNLPPSLLIPLFD
                     LTQSSDDSIAVVIDVRGRQFFDDLNKTLAELAERGISRKIIFVDASDDVLVRRFESSR
                     RPHPLQGSDRILDGIEKERERLRELRSGADLVVDSSQLNVHQLERKIAELFSVDPSAS
                     LKVNVLSFGYKFGLPVDADLVMDCRFIPNPHWDPELRAMNGLDKQVSEAVLSAESVSE
                     FLERYLALFNSLGQGYLREGKKYITLAIGCTGGKHRSVAVAEELARRINGSAIDAEHS
                     VSAHAIHRDLGRER"
     gene            51968..52927
                     /gene="yvcK"
                     /locus_tag="PLW53_01565"
     CDS             51968..52927
                     /gene="yvcK"
                     /locus_tag="PLW53_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HRD36147.1"
                     /translation="MSNPKVVALGGGHGLAATLSALRKITNELTAIVTVADNGGSSGR
                     LRKEFNSLPPGDLRMALAALCADDEWGRSWAEIMQYRFTSKGELNGHALGNLLLTALW
                     DSESDPVKGLDKVGALLKVVGRVLPMALEPLDIEGTFKTWQGTHLIRGQQEVALGRGI
                     LEDLRLIPDDPTPTSQGVDAIAKADFITLGPGSWFSSVLPHLLVTKQRQVLESSRAKK
                     VIILNLDASPSASGDELAGSSPADHLRILRRFAPDLNCDVALLDSSIAARSELLIELE
                     GLVEAMGGRVFVADLASHPGSNHHDTEKLFLTLSHIFQAEMLR"
     gene            52933..53910
                     /gene="whiA"
                     /locus_tag="PLW53_01570"
     CDS             52933..53910
                     /gene="whiA"
                     /locus_tag="PLW53_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRD36148.1"
                     /translation="MAMTAAVKDELSRLTISKPCCRKAEVSAILRFAGGLHISAGKIM
                     VEIELDTAQAARRIRKDIADIYGHDSDLAVISAGGLRKGSRYLVRVIKDGDNLARQTG
                     LVDSHGRPVRGLPPQVVSAGICCAEAAWRGAFLAHGSLTEPGRSSALEITCPGPEAAL
                     ALVGAARRLTIAAKAREVRGVDRVVIRDGDAIGVLLTRLGAHESVMAWEERRMRREVR
                     ATANRLANFDDANLRRSARAAVAASARVARAMEILGEDIPDHLREAGQLRITHGQASL
                     EELGSLSTPPMTKDAIAGRIRRLLAMADKRASDLGIPDTEAGLSPELLN"
     gene            54050..55054
                     /gene="gap"
                     /locus_tag="PLW53_01575"
     CDS             54050..55054
                     /gene="gap"
                     /locus_tag="PLW53_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRD36149.1"
                     /translation="MLVKVGINGFGRIGRNFFRAALTSKADIEIVAVNDLTDIATLAH
                     LLKYDSILGRLGQEVSHNENSITVGNKSFKVFAERDPAALAWGSVGADIVVESTGHFT
                     KAADAKKHLVGGAKKVVISAPASDEDVTLVLGVNDSAYDPAKHQIISNASCTTNCLAP
                     MAKVLHDNFGIIRGFMTTVHAYTNDQVILDFPHKDLRRARAAATNIIPTSTGAAKAIG
                     LVLPELKGKLDGYALRVPVPTGSITDLTVELSREVSAAEINEAMKKAAAGPLKGILYY
                     TEDPIVSSDIVTDPHSCIFDAGITKAIGNQAKVAGWYDNEWGYSNRLVDLMSFIGKSL
                     "
     gene            55051..56250
                     /locus_tag="PLW53_01580"
     CDS             55051..56250
                     /locus_tag="PLW53_01580"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRD36150.1"
                     /translation="MIQGLSSLNPAGKRVLLRCDLNVPLKDNGDGKRVITDDGRIRAS
                     LPTIQELLSLGASVVIIAHLGRPKGEVKRELSLEPVAKRLAELLGRQVAFSSQIVGPE
                     AFEKAAALKSGEVLLLENIRFNASETSKDETERLALAKELAKLGDLYVGDGFGAVHRK
                     HASVFELAQLLPHAAGKLIAAEVAVLEKLTQSPERPYGVVLGGAKVSDKIGVISNLLD
                     KVNILAIGGGMLFTFLAAQGKEIGKSLFEAESVDLVKQLLERAEKLGVRVLLPSDIWV
                     APAFANESPSLVSADQIPSNQMGLDIGPESAHAFASAIKECATVFWNGPMGVFEFPNF
                     ASGTRIVAQALSEVSGLSVVGGGDSAAAVRALGFSDSDFGYISTGGGASLEYLEGKQL
                     PGLIALR"
     gene            56276..57055
                     /gene="tpiA"
                     /locus_tag="PLW53_01585"
     CDS             56276..57055
                     /gene="tpiA"
                     /locus_tag="PLW53_01585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRD36151.1"
                     /translation="MSTRKPLIAGNWKMNLNHLEAIAVTQKLAYSLEDRDFDAVEVAV
                     LPPFTDLRSVQTLVDGDRLKLSYGAQDISPEKSGAFTGDISGAMLKKLDCTYVLAGHS
                     ERRAIHGESNELINAKMRAILDNEMVPIFCIGEELAMRESGAHVTYVINQLRQGLKGF
                     HKPDLKKIVFAYEPVWAIGTGKTATPDDAQEVCAAIRLELADIGSDEIAQNARILYGG
                     SVKSANIVEIMRQSDIDGALVGGASLDAEEFARLCKFHRVL"
     gene            57125..57352
                     /gene="secG"
                     /locus_tag="PLW53_01590"
     CDS             57125..57352
                     /gene="secG"
                     /locus_tag="PLW53_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRD36152.1"
                     /translation="MSLALSILLVITSILMILLVLLHKGKGSGLSDLFGGGMSSTYGG
                     SSVVEKNLDRITIVVGGLWFAIIIALGLVLK"
     gene            57388..57735
                     /locus_tag="PLW53_01595"
     CDS             57388..57735
                     /locus_tag="PLW53_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HRD36153.1"
                     /translation="MAGGSAIRGSRVGAGPMGEAERGEAIARFHVSYWCQNGHETKPS
                     FAEDGTVVVPAEWDCPRCGFPAGQDKNNPPSPIKNEPYKTHLAYVKERRTEAEAKKIL
                     EAALKKLRAEDED"
     gene            complement(57732..59249)
                     /locus_tag="PLW53_01600"
     CDS             complement(57732..59249)
                     /locus_tag="PLW53_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36154.1"
                     /translation="MIKLTGFKQGRGLWEKLDKATIRLANCDPTIWGESAAKEAAIRL
                     NWVNLPEKSRQLLPELDALSAWSREFGHEVFILCGMGGSSLAPEVMAQVYKKELKILD
                     STDPAHVKKVLDQDLSKSCIIIGSKSGSTIETASQMAAANQQLIKQGLDPKNHFVVIT
                     DPGSPLDIQARESNLRVVNADPNVGGRFSALSAYGLTPAALIGIDISILLDDAYEASQ
                     SFAKPGSVVTQVAAALADKFFSFAGFLDTGSNVSGLSEWIEQLIAESTGKDGKGVLPI
                     TLTKKSKLNYPVITFDGSGENSVEASLGEHFIFWQWVTALLGYLLQVDPFNQPNVTEA
                     KDKTNALLSRWSKGREEIKPVFESGSIAVYSTLQEDSVAHYLKRAIANSKGYLAIMAY
                     LHRGIDDQIKDLAPLLESKSKLPATFGWGPRFLHSTGQFHKGGPSTGSFIQVTADNEN
                     NWPIPGRDFGFETLIMAQALGDLEALSSRGFSVIRFHLKNRKQGIAELLSAAEKI"
     gene            complement(59246..60355)
                     /gene="tal"
                     /locus_tag="PLW53_01605"
     CDS             complement(59246..60355)
                     /gene="tal"
                     /locus_tag="PLW53_01605"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRD36155.1"
                     /translation="MSKTLAQISQAGTSIWLDDLSRERMIDNGKSRSLKSLITEESVV
                     GVTTNPAIFSTSISQSSLYSDDLKALAAKGYSAEQIITELTCSDVAKACDLFREVFLA
                     TGGVDGRVSIEVDPRFARDTKKTISQALDLWKKIDRPNLLIKVPATIEGLPAIQTLIS
                     EGISVNVTIIFSTKRYEQVLDAFIKGLEDRARKSLPLSDIHSVASFFISRVDTEIDPR
                     LKASASKEALDLLGKSAIANAHLAYQHFEKVLHSDRWQVLAKLGGNIQRPLWASTGVK
                     DPNYDPTQYVMELVAPFCVNTMPEQTLNAVKAQGEFKGNTISGKYLDASGVINQLASI
                     GIDIEEVAEKLEVEGIDKFIKPWLELIANVEAASK"
     gene            complement(60352..62412)
                     /gene="tkt"
                     /locus_tag="PLW53_01610"
     CDS             complement(60352..62412)
                     /gene="tkt"
                     /locus_tag="PLW53_01610"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRD36156.1"
                     /translation="MQPELAPWIDLDEKAVAIARGLAMDAVQKVGNGHPGTAMSLAPV
                     AYTLFQRFLRHDPKDPNWIARDRFILSCGHSSLTLYIQLLFSGYGLTLDDLKAFRTWG
                     SLTPGHPEFGHTLGVETTTGPLGQGVANAVGMAMAARYERGLLDPDTPKGESVFDHNI
                     WVICSDGDLQEGVSAEASSLAGVQELGNLKVIYDDNRISIEGDTHYAFTEDVSMRYRS
                     YGWHVIEVSAKADGDVDRVAIEAAMNEALLVTDKPTLIRLRTVIAWPAPKARNTAKSH
                     GSALGADEVAATKIELNLPAQDFYFPEEVEKHVRKVQERSLKLKSNWNADFSKWQSAN
                     PERAALLERLRKRELPTNWNADLPVFASDKEVSTRKASGDVINALAAKMPELFGGSAD
                     LAESNNTTIEGGGSFLPSTSKMKGASPYGRIIHFGIREHAMGSILNGIALHGLARSFG
                     GTFLVFSDYMRGAVRLAALMKLPVTFVWSHDSIGLGEDGPTHQPIEHIASLRAIPGLD
                     VIRPADANEVVACWSQIVKRDKTAGLLLSRQNLPVINRTLYNSTAEVWRGAYALNDVS
                     TPDVLIIATGSEVSIALKASEELSQSGIKARVISAPCLEWYREQDQGYKDLLMPKSVK
                     ARVSVEAGIAQGWHELIGDCGVAISLEHFGASASAPTLFKEFGFTPEAVVKACQESMA
                     KSSK"
     gene            complement(62412..63320)
                     /locus_tag="PLW53_01615"
     CDS             complement(62412..63320)
                     /locus_tag="PLW53_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRD36157.1"
                     /translation="MAPLADKALLKLSTLMVFTQSAIVCTGALVRITGSGLGCSTWPE
                     CTPGSYTPTPDQPEAPLHAWIEFGNRLLTFVLLINALALMFTILKSGKRELRRLGALQ
                     ILGILAQGVLGGITVLTALNPATVAAHFLLSIILIAGALSLRQRAHGKSPIEITLIPL
                     VTKLIWLHLLLTSLVLIAGTIVTGSGPHAGDSAAERFNLDSRTMAWIHADLVIALLGV
                     SIALLIAIRLGLTGQARQVLSGRIQIFLIVALAQGSIGYIQYFTKLPEALVAAHIIGS
                     IAVWLSAWNLFISSNLGANLIARKGL"
     gene            63391..64095
                     /locus_tag="PLW53_01620"
     CDS             63391..64095
                     /locus_tag="PLW53_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRD36158.1"
                     /translation="MKSTLAPSEDLRTKDQVARAILENGPSTAVALAASLGLTPAGIR
                     RHLDSLVSEGILEEREPHIRSAGLRGRPSKVFVMSDKGRERFEHSYDDLAVSALKYMA
                     ASAGAHLVDGFAKHRADEIIRRSKKTVTGSKNAVDGLVKFLSDEGYAANVHPKAIGLE
                     LCQHHCPIAHVAAEYPQLCEEETAAFSKILGTHVQRLATIAHGDGVCTTFIPQLGSKG
                     KKIEKTQTSNRKQSKR"
     gene            64092..65504
                     /gene="sufB"
                     /locus_tag="PLW53_01625"
     CDS             64092..65504
                     /gene="sufB"
                     /locus_tag="PLW53_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRD36159.1"
                     /translation="MSQIAHPELEGIGNYEFGWSDKNDAGANSKRGLNEEVVRDISAK
                     KNEPAWMLELRLKGLALFDKKPMPNWGSDLSGIKFDTIKYFVRSTEKQATSWEDLPED
                     IKNTYDRLGIPEAEKQRLVSGVAAQYESEVVYHSIREDLEKQGVIFLDTDTALREHED
                     IFKEFFGSVIPVGDNKFAALNTSVWSGGSFVYVPKGVHVEIPLQAYFRINTENMGQFE
                     RTLIIADEGSYVHYVEGCTAPIYSSDSLHSAVVEIIVRKNARVRYTTIQNWSNNVYNL
                     VTKRAICAEGATMEWIDGNIGSKVTMKYPAVFLMGEHSKGETLSIAFAGEGQHQDAGA
                     KMVHAAPHTSSTIISKSVARGGGRTSYRGLVQIKEGAHHSKSTVKCDALLIDTISRSD
                     TYPYVDVREDDVTMGHEATVSKISDDQLFYLMSRGLEEDEAMAMIVRGFIEPIARELP
                     MEYALELNRLIEMQMEGSVG"
     gene            65504..>65796
                     /locus_tag="PLW53_01630"
     CDS             65504..>65796
                     /locus_tag="PLW53_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36160.1"
                     /translation="MSSVLPTNYLTPTGREEAWRFTPLKRIAGLHDPAVSVIDRISIE
                     LFGSARAGFNISTVKASELPAKSETTDAIVQRLRSEVTEVLHLKIDNEAVINEP"
     assembly_gap    65797..65896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            65903..66211
                     /locus_tag="PLW53_01635"
     CDS             65903..66211
                     /locus_tag="PLW53_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRD36161.1"
                     /translation="MESIEIKVDQLQESKPVKFTLGSEDVCVARIGDEVFAVADICSH
                     AEASLSEGDIKDYKIECWLHGAEFDLRSGEVLTPPAFEAIETYAVVRDGDTVTISRKK
                     "
     gene            66231..66983
                     /gene="sufC"
                     /locus_tag="PLW53_01640"
     CDS             66231..66983
                     /gene="sufC"
                     /locus_tag="PLW53_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRD36162.1"
                     /translation="MAQLVIKGLQVSVEGESGAIEILRGVDLTINSGEVHAIMGPNGS
                     GKSTLAYSIAGHPKYSVTGGSVTLDGVDVLEMSVDERAKAGLFLAMQYPVEVPGVSVS
                     NFLRTAATALRGEAPKVRTWVGEVKEAMSKLSIDPTFSERSVNEGFSGGEKKRHEILQ
                     MELLKPKIAILDETDSGLDVDALRIVSEGVNRVKSEQNLGIMLITHYTRILRYIKPDF
                     VHVFAGGKIVEEGGPELADRLEEKGYAQYAKA"
     gene            66994..68247
                     /locus_tag="PLW53_01645"
     CDS             66994..68247
                     /locus_tag="PLW53_01645"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRD36163.1"
                     /translation="MSKLNLSAIRADFPILKREIRGNNRLIYLDSGATSQKPNSVIAA
                     ERDFYENHNAAVHRGAHQLAEEATELYEGARAKVAAFINADVDEVIFTKSATESINAI
                     AYSLGNARPGSKFHIKPGDRIVVSEMEHHANLIPWQELSARTGAELVWFKVSDNGRLD
                     LSNMDELINERTKVVALTHQSNVLGTIVPLDQVTAKAHSVGALFVLDACQSVPHYKVD
                     VKALNVDFIAFSGHKMLGPTGVGILWGKKNLLDEMPPFLTGGSMIETVTMERATYLDA
                     PKRFEAGVPNMAQAIGLGAAVDYLNAIGLDEIHAHEVELTKAALDGLQSIKGLSVIGP
                     KDLEMRGGVVSFAVEGVHPHDLGQALDQYGIAVRTGHHCAWPLMRRFKTVATTRASFY
                     LYNDFDEIQALIDGVERARKYFESR"
     gene            68247..68693
                     /locus_tag="PLW53_01650"
     CDS             68247..68693
                     /locus_tag="PLW53_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRD36164.1"
                     /translation="MELDSLYQEVILEHYKRPLNKVLKPNPTIQVQHINTSCGDEITL
                     NLHTDGKKVIEVSWEGIGCSISQASTSVVSDLVMGKEIAEVLKIIDSFQGMVLSKGTD
                     SGNEELIGDGVAFAGVSKFPARVKCALLGWMATKDAILQATSSEGK"
     gene            68697..69008
                     /locus_tag="PLW53_01655"
     CDS             68697..69008
                     /locus_tag="PLW53_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRD36165.1"
                     /translation="MSASRDDVTEAMKDVIDPELGINVIDLGLVYDVSIDDSNVAVLD
                     MTLTSAACPLQDVIEDQTKSVLQDLVTDVKINWVWMPPWGPDKITDDGREQLRAIGFT
                     V"
     gene            69049..70911
                     /locus_tag="PLW53_01660"
     CDS             69049..70911
                     /locus_tag="PLW53_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36166.1"
                     /translation="MSQMAVWMSFRSFTADSSVKNQKLKPGTVKRIFGFALPYKSFLI
                     FFLITVVIDAFLIVTTPLLLRNLIDDGVIPGNSALVTQIALLVAFIAILDALFNMIGR
                     WFSARIGEGLIFDLRSQVFTHIQKQSIAFFTRTQTGALISRINSDVIGAQQAFTSTLS
                     GLISNVLSLILVTVTMLILSWQITLVSLLLLPAFIIPTKWVGKKLQGLTRDSFNLNAE
                     MSATMTERFNVSGALLVSLYGNPSSESSTFSARARKVANIGISIALLNRLFFIALTSI
                     AAIATAFAYGVGGHFTINEQISLGTLLAITALLARLYGPLTALSNVRVDVMTALVSFE
                     RVFEVLDLQPMVSDKPDAKPVGSGGLSIKFDSVRFSYPRAEEISLASLESVAKPETVD
                     SGEVLKGISFEVLPSTMTALVGSSGAGKTTISALLPRLYDVTSGSIAINGVDIRDLQV
                     QSLRSSIGVVTQDAHMFHDTIEANLRYAKVDATTSEMEKACQDAQILELINSLPNRFE
                     TVVGERGHRLSGGEKQRLAIARLLLKSPRIVILDEATAHLDSENEFLVQAALANALKD
                     RTSIVIAHRLSTIRAADQILVLDNGLIAERGKHDELLALNGMYAELYSRQSFGA"
     gene            complement(70893..71612)
                     /locus_tag="PLW53_01665"
     CDS             complement(70893..71612)
                     /locus_tag="PLW53_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRD36167.1"
                     /translation="MRRLITVSTLALLLIFGCVQGALWQYERYEFRHANNELIRKNVS
                     IAALLTEDDLSSKTASEIAWRSISLVGNFDPSREFLIRNRYHEGKYGFGVITLFISDS
                     GKRYWIDRGWVIAGKDAQTPPVVQKVDSLPVEITARVRTSEIESRVQGSVFALPGADS
                     TPKLVKWNSEQAIETEPIYFDLISSSNPEVTPEVATALPELSDGPHLAYSFQWILFIF
                     LVLFAWYLVIREDRKTQAPKL"
     gene            complement(71629..71928)
                     /locus_tag="PLW53_01670"
     CDS             complement(71629..71928)
                     /locus_tag="PLW53_01670"
                     /inference="COORDINATES: protein motif:HMM:NF022742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HRD36168.1"
                     /translation="MALKRWNLAMPKRRNYQNITEAGAALSDDLPSRQKRYFISMMIR
                     TACFLLAVFTPSPYRWFFLVGAVTLPYISVIMANGGRETIRGKSAVLRSKQREIG"
     gene            72119..72715
                     /gene="infC"
                     /locus_tag="PLW53_01675"
     CDS             72119..72715
                     /gene="infC"
                     /locus_tag="PLW53_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRD36169.1"
                     /translation="MLLVKGRVISTTDPRINDRIRVPEIRLIGALGEQVGVVAIDKAL
                     KMADEAGLDLVEIAPEAVPPVCKIMDFGKYKYEIAQKAREARANQTHIVVKEIRMGLK
                     IENHDYETKKNHIEKFLLSGDKVKVTVQFKGREQTRPEMGFRLLQRLAEDVATSGFVE
                     FAPKREGRSMTMVLGPVRKKSEALAEAKRARASKAKEV"
     gene            72739..72936
                     /gene="rpmI"
                     /locus_tag="PLW53_01680"
     CDS             72739..72936
                     /gene="rpmI"
                     /locus_tag="PLW53_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRD36170.1"
                     /translation="MPKMKTHSGAKKTFRVTGSGKIVHERAGKRHLLERKSSRVTRRL
                     SKDQTANDRNTFSAKRLLGMK"
     gene            72949..73311
                     /gene="rplT"
                     /locus_tag="PLW53_01685"
     CDS             72949..73311
                     /gene="rplT"
                     /locus_tag="PLW53_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRD36171.1"
                     /translation="MARVKRGVHAAKKRRTTLERASGYRGQRSRLFTKAKEQVTHSLV
                     YAFNDRKDKKGDFRQLWITRINAASRANGITYNRFIQGLKSAGVEVDRRVLSELATND
                     PAAFTALVEVARKNVKVS"
     gene            73331..74134
                     /locus_tag="PLW53_01690"
     CDS             73331..74134
                     /locus_tag="PLW53_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD36172.1"
                     /translation="MITSLHSPHVEAVKALLGSRGGKARKESGQYVIEGLSSIKEALD
                     FSPEEITTLYLTSDGMSRLATIPEGYFEIVEVSPEVMKAMTDTVTPQGLLAIAQIPQN
                     SFEDFLAASKSELKIAYFWQIQDPGNAGTVIRAADAFGFDAVIFSNNSVDIYSPKVVR
                     SSAGSHWHIPLFTSISEGELKTQLSGKAFDIYGTDASGGLELLEAANESKNRSAIWVF
                     GNEARGLDAKVISTLGAKQVAIPISGKAESLNLATAASVVMYAVSAARK"
     gene            74170..75186
                     /gene="pheS"
                     /locus_tag="PLW53_01695"
     CDS             74170..75186
                     /gene="pheS"
                     /locus_tag="PLW53_01695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRD36173.1"
                     /translation="MSLDNAGIEQIKADALSAIKNSQSLDELREVRIAHVGDRSPIAR
                     ANQGLAEIEIEKRAAMGKLIGTARANINQAFDDKEKELTALRDSRALSNERVDVTLAS
                     NRNPRGALHPLTLLTNEISDLFVGMGYTVQEGPELEASWLNFDALNIAEDHPARTMQD
                     TFFIEPLSSGLVMRTHTSPVQIRTMLENKPPIYVICPGRTYRADELDATHTPVFNQVE
                     GLVVDKGITMAHLKGTLDHFAAKIFGPDVTTRIRPSFFPFTEPSAEVDFFYNGRWVEW
                     GGCGMVNRKVLQAAGIDTDIYTGFAFGMGLERTLMVKYGITDMHDIVEGDVRFSRQFG
                     SGGR"
     gene            75183..77660
                     /gene="pheT"
                     /locus_tag="PLW53_01700"
     CDS             75183..77660
                     /gene="pheT"
                     /locus_tag="PLW53_01700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRD36174.1"
                     /translation="MKLPLSWLSEFVEFPKTISVADIAAGFVKVGFEVESIDNPAEKI
                     KGPLLVGKVLTIEELTGHKKPIRYVGLDCGEAKTRYVICGATNFVAGDLVVVALPGAI
                     LPGNFAISARETYGKTSNGMICSSKELGLGDDHSGIIVLDSKAKIGSPALKVLGADDP
                     IIDIAVNPDRGYAMSVRGAARELAMALGLGFKDISSKALIASLEKKKKRGKITPVQIS
                     DKTGADQIYLRSLSNVDPLKPTPQWMARRLSQAGMRPISIAVDITNYVMLELGQPLHA
                     FDSAKISGTLRVARAGKFTELITLDKVKRKLAKDNLLITDDKKVLALAGTMGGLDSEV
                     SEFTVGITLEAAHFDPMSIARNSRSHILSSEASRRFERGVDPLLAELASARAALLLIE
                     YAGAIYNGSSVKKTPLKKRSITISASEISSLIGTTYSDKEIEKSLKAIGASLRKSGKK
                     WVVTPPSWRPDLNELPDLAEEVARFFGYDRIPSQLPPVKLASNGSSGLTPMQKRRRSM
                     SLKLANRGLVEVHNYPFVSQSQMDLFGFTGDRAKTFKIANPMSDEYPYLRTHLTPGLL
                     AAAGRNLSRGEKSVALFESGAIFRNIETLKPAGSVSVSKRPTDSQIKQIYQSVPKQPL
                     HIAGVIAGEITNSGWWGKGRVVEWSDAVDLVRELIEDSGNDYSIEAVDLAPWHPGRCA
                     EFRVDGKAVAHAGQIHPRVTSALALPDATVAFALIVDALKFPDNFKASPISVMPAAIQ
                     DISLFVDQKVPAEKVAAALREGAGELLESIALFDRYQKEGEAQVSLAFSLVFRASDRT
                     LTSDEVSELRAKAGAMASKKCGAQIRT"
     gene            77715..78734
                     /gene="argC"
                     /locus_tag="PLW53_01705"
     CDS             77715..78734
                     /gene="argC"
                     /locus_tag="PLW53_01705"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRD36175.1"
                     /translation="MKIGVLGASGYAGGELLRLLSGHPDFEVSYIAAGSNAGEKITDL
                     HSHLITYGDQRFSGTDIGAINNCDLIFLALPHGQSAEIVKQIDEKVKVVDLGADFRLK
                     SEQAWKQYYNDSYAGAWAYGLPELPGKRELIKKSSRVANPGCYATAIALAAAPAVLAG
                     GVDAGDIVVVAASGTTGAGRSTKVSLSASEIMNSMVSYKFGGVHQHTPEIEQTLAELG
                     SKEVKISFTPILAPMPRGILATVTAKTTLSEDEIRKSYEKMYQDEPFVHLLSKGQLPE
                     TSSLTGSNSVQMQVAVDAHTSRLIVSVAIDNLGKGAAGQAIQNANLISGLVETTGLSA
                     LGIGQ"
     gene            78731..79900
                     /gene="argJ"
                     /locus_tag="PLW53_01710"
     CDS             78731..79900
                     /gene="argJ"
                     /locus_tag="PLW53_01710"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRD36176.1"
                     /translation="MTLKSEYSQSLPKGFRGSGVSAGLKSSGAKDLALIVNDGPDNFA
                     SAVFTTNQVVAAPVIWSRQVLKDNQVSAVLLNSGGANAATGADGFADTHKSAEEVASL
                     LSLSSSDVAICSTGLIGVRLDMEKLLAGVKSAHSALSTDSTEDLARAIMTTDSKPKIA
                     TYESSGVSFTGIAKGAGMLAPSLATMLSVVMTDAKVDKAVADEIFAKVCEKTFNRIDS
                     DGCTSTNDTVLFLSSAASGVELSNEQLEQALFAVAASLSRQLIADAEGHSKIISITTI
                     NAKSERDAVEVGRACARNNLLKCALGGEDPNWGRVLAAIGTTNAVIDPLNIDVDLNGV
                     KVCKSSSPGEDRAKVVMTGELIEILIDLKVGSSSATIWTNDLTASYVHENSAYST"
     gene            79897..80655
                     /gene="argB"
                     /locus_tag="PLW53_01715"
     CDS             79897..80655
                     /gene="argB"
                     /locus_tag="PLW53_01715"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRD36177.1"
                     /translation="MIVIKFGGHAMGEHSRKWASEIASRFKLGERFVIVHGGGPQIDR
                     ELARRGIEKSSVNGFRITTPEIMEVVEFVLTGSVLRSVVRDLIAAGLPAVGITGSDNQ
                     LLEVELRDEAKFGLVGKIKRVNPKIINDLLDMGYLPVISPVANDSSTRALNVNADIAA
                     GAIAGSLRANEALFLTDVPGIYSAWPDRSSLISEITISDLKKISFEGGMVPKVEAVIN
                     AIESGASSARVIDGTSLPAFVDALSGDGGTLVKP"
     gene            80652..81833
                     /locus_tag="PLW53_01720"
     CDS             80652..81833
                     /locus_tag="PLW53_01720"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HRD36178.1"
                     /translation="MKKGNKELLKKWNSVMQSNYGTPSISLSKGKGIEVWDLDGVKYL
                     DFLGGIATNLLGHNHPAIVAAVSKQIRQLSHVSNFYAHPVALELAEKLQKMTTDKSAR
                     VFFCNSGAEANEAAIKIARLHRGKRIVAATGAFHGRTMGALSLTGQSSKRDAFKPLLP
                     GVKHVKYGDIKALRRAVNKRTSMVILEPIMGEAGVVTPPSGYLKAARQICDENGALLA
                     IDAVQTGMGRCGSWFGYEDEGIKPDLITIAKGLGGGLPLAAMITLGKASELFKPGSHG
                     STFGGNPVAAAAAIAVIDEIEKKKYLALNTVKGALIRNLISPIIGVEQVRGKGLLIGI
                     CVKESKAKQIAAEMQSKGFLVNAASDDVIRIAPAYVVSEKQIMSFVSAFNSSCEEIYR
                     G"
     gene            81826..82359
                     /locus_tag="PLW53_01725"
     CDS             81826..82359
                     /locus_tag="PLW53_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HRD36179.1"
                     /translation="MAKRGTLSSNARRALVVNFVNQGLVHSQGDLVELLAEEGIEVTQ
                     ATASRDLDDVGAVRGKDSSGVMRYQFLDSSVEPLARMARVSDQLLLSITASGNIVVIK
                     TPPGGAQLLASALDRASESSELSSAIGTIAGDDTVLVISRNASGGKLLAAELRDYITT
                     EFSSNARKTSKSKKARR"
     gene            82359..83786
                     /gene="argH"
                     /locus_tag="PLW53_01730"
     CDS             82359..83786
                     /gene="argH"
                     /locus_tag="PLW53_01730"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRD36180.1"
                     /translation="MALWGGRFSKEAGEAMFALSRSVHFDWRLASYDLRSSLAHLNSL
                     EASSIIDKALALKIRSAIKELQSEVASGKFAPIASDEDVHSALERGLIEKLGAEGGAI
                     RAGRSRNDQVATDFRLYLIDHMIEIALETLKLSSAITDLAGEYAGDVAPGFTHLQHAQ
                     PVSFGHELAKHAHSLSRDVSRISDWLTRSNLSPLGAGALAGSSLPLDPESSAKELGFS
                     GAIANSIDAVSDRDFVAEALFVISLIGIHLSRIGEEWSLYASSEFAWVELDDAYATGS
                     SIMPQKKNPDAAELARGKAGRFIGSLTGLLTVLKGLPFAYNRDLQEDKELTFDAVENL
                     LLLLPAITGMVATSKFDREKMRAAAPLGFSLATEIADFLVLKGVAFPIAHDAAGKAVR
                     RCEELAIELDQLSDLDLKSIHPLLSGEVRSRLNLDAALAARASASGTAPESVKKQIAN
                     LRVKISADASEFAGERKRFSGMMDQ"
     gene            83783..85057
                     /gene="tyrS"
                     /locus_tag="PLW53_01735"
     CDS             83783..85057
                     /gene="tyrS"
                     /locus_tag="PLW53_01735"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRD36181.1"
                     /translation="MSKALLDDLTWRGSIAQSTDRAELEKYLANPGASLYLGFDPTAP
                     SLHIGNLVALTLLRRFQLAGYKPIALVGGATGLVGDPSGRSAERSLNEADLVADWVER
                     IRKQLEGFLDFTGKNAAIMANNLDWTAPVSAIEFLRDIGKHFSVNQMLAKDSVATRLE
                     SGGISYTEFSYQVMQAFDYLELFRRHNCALQIGGSDQWGNIVAGVDLIRRVEAKSAHA
                     LTIPLLAKADGTKFGKTAEGAIWLDPTMTSAYALFQFFLNSDDRDVEQLLKTFSFKSR
                     AELEALFESLKTNPGAREAHRELAREVTTLLHGADQARKVEAAAKALFGQGEIRDLDI
                     ETLKSALAELPKARINKGAEIPSWVDLITSSGVVESKSAARRVIKEGGAYLNNEKITG
                     EDFAPKSSDLLHGQFLLLRKGKRDLVAVEITD"
BASE COUNT        24312 a        17427 c        20412 g        23080 t
ORIGIN      
        1 gaagagaatt atcaaggcgt aggttggcag caagcccata gcatcatggg acttgcttca
       61 ggaggagcag taggaaccgg tcttggaagt agtaagcaga agtggggaag ccttcctgaa
      121 gttcacaccg acttcatatt tgctgtcatt ggagaagagc taggtttact tggaacgcta
      181 gcaactcttg gtctatttgg aattttgatt ctgggaatat ttaaagttgc aattaatgcc
      241 cagaactctt ttgatcgtta tgtctgtgcg ggcattggat gttggatctc agttcaggtc
      301 cttcttaata tcggaactgt aattagcgta attcccgtag ttggagtaac gctgccactg
      361 atttcctacg gcggctcctc acttattgca accttcatcg cgcttggctt tgtacttggg
      421 gttttgcgac gcgaccctca aattgcctca gagattcgcc agcgtaaggc ggcaagaact
      481 gcgagaggat aaagcccatg gcgcagcgtg tggtctttgc aggcggggga acggccggac
      541 atattgagcc agccttagcc gttgccgagg cgctattact aaaagattca aaactaactt
      601 gcgaattcat aggcgttaga ggtggtctgg aatcactttt gattcctaag agaggctatc
      661 gaattcatta cattccaaag gcttcatttc caagaaagat ttctccaaaa atcttcctct
      721 ttcctattct tttagtaggt gcaatcttta aagcaagatc gataataaaa ggatcagatt
      781 tagtaattgg ctttggtgga tatgtatcaa ctccagcata ccttgcagct tttagccgaa
      841 agattcccat tttgatccat gaagcaaatg caaaacccgg gctagctaat cgccttggaa
      901 ggcggctagc taaagtaact gcagtcaact tcgaaaaagt tgcaaaacaa tggtcaggtt
      961 ctgttttaac tggaatgcca attcgccaat cgctaaatag tcttgcagag cttcctaaca
     1021 aatcaagttt caagcaactc aattgtcaga gttggggatt tgatgccaat cgcccagtag
     1081 ttgctatctt tggcggctca cttggttcaa ctcatatcaa ttcagtgatt gcagactttt
     1141 taaaaagata ccaagataaa taccaagaag ttcagattat tcatgctctc ggaataaata
     1201 atgaactgcc gaaggcttca agtaattatc ttcctcagcc ctactttcat gatatggctt
     1261 cgatatatgg aagcgctgat ttactaatca caagatctgg ggccgtcacc tgctctgaac
     1321 tatcctctct tggcaaatat tcgattctta ttcctctgcc tcatggcaat ggagaacagt
     1381 ttgataatgc agctgctcta gtggctcaag gttcagccat gatggtctca aatgatgact
     1441 tttcagcaga gtggcttgat aaaaatcttg cagcagcatt aacaagcgcg gagaagtatc
     1501 aggctaaatc agtaggtagc aatgccaagg ctgcgatgag aattgcagag cttgcccaag
     1561 agatattgct aggaaaatag ggctaaatga gcaatcaagt gattgttgag tggagcaaag
     1621 ctcgagtcca ttttgtcggc ataggcggct cgggaatgag cggtattgca agaattatgg
     1681 cttcgcgcgg agcagaagta tcaggctctg atttacatga ctcatcgact ttaaatggcc
     1741 tacgaagcct tggggccact attcatattg ggcatagcag agagaatata aaaagcgcag
     1801 atctagttat taaatcaagc gccattcctg caagcaatcc cgagcttgaa gaagcagaga
     1861 gtcggggcat tccaattttg gagcgagctg ccgctctggc gcaactaatg attggtactc
     1921 gctctgttgc tgttgctgga actcatggaa aaactaccac cacatcaatg ttgacagttg
     1981 ctctgcagca ttcaggcctt gatccatctt ttgcaattgg agcaacggta agtaactctg
     2041 gaacaaatgc ccatcagggc tctggcaata cttttatcgt tgaggctgat gaatcagatg
     2101 gctcctttac cgcctataaa ccgctagggg caataattac caacattgag ttagatcatg
     2161 tagataactt tgcaaccctt gctcaaattg accagatctt tgatgacttt gttgccacta
     2221 ttaggccaga tggtttttta gttctatgta ttgatgatcc tggcgcactt cgtttaatgg
     2281 agcgagtgaa gaagaaaggt tcttcaattt ccatctccac ctatggaata gatagcgatg
     2341 cagatctgcg acttgatcga attcatctgc aaccaaagag cgccgaggca agagttagtt
     2401 ataaaggtag agttttaggg cagatggaac ttgccattac gggccggcat aatctactca
     2461 acgcctgcgc tgccctgctg gcaggccttg agcttggcgc aaatgcaaat gatctaatca
     2521 aaggattagc tctctttagt ggtgccagaa ggcgctttga aattaaagga atttctaagg
     2581 ggatcacagt cattgatgac tatggccatc atccaacaga aatcaaggca acccttgaga
     2641 gcgcaaggat ctatgctcaa agtgggcgag tactgacagt tttccagcct caccgctact
     2701 caagaacgca agtatttgcc agtgaatttg ctaaagcact ctctgcatct gattacacat
     2761 acctgcttga aatctatcct gcaagtgaaa aagagatccc cggagttagt tccctactaa
     2821 tttcaaaaga aatggcaagt agcaaccata gctatcaacc ttcaatgcca gaggttgtgg
     2881 aagctgttgc aaagatggcc aagagtggcg atttaatcct taccctagga gctggagatg
     2941 tgagctctct aggtaaattg attctagagg caatcgaatc ctaattgcat gaagttgaat
     3001 ccaagattaa ttctacctat ctcgatattg ctcattggag cgctggcata tctattgggc
     3061 tttagccaag tgtttgtggt gaaagaaatc aagattgata ttaaagataa gtcaattgca
     3121 agagaggtcg aagatcgact ctcccaacca cctgcggtag tcaaactggg agatcagatt
     3181 gcaagggttg atcgtcgaga aatagccaat cgactccgtc aatttgcctg ggttgaaaat
     3241 gttgagttgg accgaaattt gattaatggc aaattactaa taaagataat ttcaagaact
     3301 cctatcgccc gactgacctc aaccacgagt cctcagagcg tcagtatcgg gtttatgggc
     3361 gaagaccttg attacttcta tcttcctaga caagctattg atcaggccat cagctcagga
     3421 gataagagtt ggcagcagat accggagtta actctgcccg ctgggtcaag tgatggggca
     3481 gataaccaat taaaggctgc ggcccgggag ttgatagagc tatttaacca agagcttgcc
     3541 ccaagaggat ttgaattaac gcgaattaac gcaagagata acgccagctt catcacctct
     3601 gctaactatt cagggcgcaa catcgaaatc tcctggggca acgtcaatga tctagggcta
     3661 aaagttgaag tactggagcg tttattggcg caagatgaga ataaaaaagt tcgaaaaatc
     3721 aatttaattg atccattaaa accaacagtt cgatagaaaa gtatttcctt aactaatcgt
     3781 gtcggtcttt gccacaggag cgctgcgcaa ctacttttgc gcgcagagta aacagaaact
     3841 ctaagtctaa agttgagaat tagggtttgg agagaggcga aaaaagtggc aacaccacag
     3901 aattatttag cagtcatcaa agttgttgga attggtggcg gcggagttaa tgcagttaat
     3961 cgcatgattg atgcggggct aaaaggcgtt gagttcattg cgattaatac tgatgcgcaa
     4021 gctcttctga tgagtgatgc tgatgtgaaa ttagatattg gaagaaaatt aactcgcggt
     4081 cttggtgcag gtgctgcgcc agaaattggc aagcaagcag caattgatca caccgaagaa
     4141 attgaagaag tacttcgtgg ctcagatatg gtttttgtta ctgcaggaga aggcggcgga
     4201 actggaactg gcggagcgcc aataattgca aagattgcaa aagatgccgg agcgctaaca
     4261 gttggcgtag taactcgtcc atttagcttt gaaggcaaac gccgcgcgat gcaggccgat
     4321 gaaggaattg aaaatttaag aaaagaagtt gacacgctaa ttgttattcc aaatgatcgc
     4381 cttctttcaa tctctgatcg ctctatctca gcaccagagg cttttaagac agcagatcaa
     4441 gttttgctct cgggcgttca aggcattact gatttaatca ccacaccagg tttgattaat
     4501 cttgactttg ctgatgtaaa gagcgttatg gatggcgccg gttctgctct tatgggaatt
     4561 ggatctgcaa gaggcgaggc aagagctact agagctgctg agcttgcaat ctcatcacca
     4621 ctacttgagg catcaattga tggcgcacat ggcgttcttc tctccatagc tggaggatca
     4681 gatttaggcc tctttgaaat tagcgaggca gcagagctag ttgcaaagtg cgcacaccct
     4741 gatgccaata ttatttttgg aacagttatc gatgacgcgc taggtgatga ggtaagagtt
     4801 accgtaattg ctgccggctt tgatggggga gcgccaaaga gactctctgt tccagtgatc
     4861 tcaactgctg ccttaaatgg tcaagcagat ccaattgcgg caaatgatcc aatcgctgtt
     4921 gcaatggata caccagtgac tccaagaaga aagataactt ttgaagagtt agtggctgaa
     4981 gatgagattg atatccctga tttcatgaag tagtaagcag ttaaatgtcg aggtcgctct
     5041 ttacctcgcg ccatggcggc ttaagtagta atgaatttga aagttttaat ctagctctcc
     5101 atgttggcga tgatgcaaaa actgttgaat caaatcgaga tctgctggct aaagaacttg
     5161 gcataaagcg aagccaatta ttctttatga atcaggttca tggttcaaga gttgttgaaa
     5221 ttgatgaaaa ctctacttac ctagatgaac cagaggccga tgccttattt actagaagaa
     5281 gtggcatcgc ccttgctgtt ttaactgcag attgcatacc acttcttctc tcctcgccct
     5341 ctgctatcgc tgcggtgcat atcggaagaa agggacttat tgcaggagtt ctagaggcaa
     5401 cgctagataa gtttgaaagt tatgggatta cttcaagtca gattagcgcg ctccttgggg
     5461 cgtcaatttg cggaacctgt tatcaggtaa gtcttgagat atatcgccag gttgtagagc
     5521 agatagagca gtgcgccaca gatgagaata agagatgtct tgacttagag gcaggagtaa
     5581 tctcgatact taaaagcagg ggaatcacct ggagctcaag gggtgagtgc agtaaacata
     5641 gccctggata tttttccttt cgtagagata acaaaactgg aaggcaggca ggagtgatta
     5701 tcagatgagc gctaggcaga tagagataaa gagcgcttta gataatatcc gttctcaact
     5761 aaaacctgga gttgaattag tagttgttac taagaacttt ccaattagcg atcttgagat
     5821 tctttacact cttggcgagc gtcaatttgg agagaatcga gaaaatgagt tgagccctaa
     5881 ggcagaggct ctgccaaagg atattgattg gcactttcag ggaactattc agagcaataa
     5941 attaagagca atagtttcag tagctagcta tattcactca cttgatgagc tacgccatgc
     6001 atctaaaatc tcagagttgg ccctggagat gggcaagaag cagagctgct ttattcaagt
     6061 taatttagat tcagaagagc tagtcaaaca agagagtaat cgaagcggga taagagcaga
     6121 gcaatttaaa gccttttctg agagcctgct aaagcttgct ggagtagaaa ttgttggagt
     6181 gatgggggtt gctcctctaa ataaagatcc tcggccaggc tttgaaaccc taaggaattt
     6241 gagtcaagaa cttttgaaag tcgctccaaa tgctggcttt atctcatcgg gaatgagtgg
     6301 ggattatcag attgcaatgg aatatggcgc gacacatatc aggctaggca gctcaatcct
     6361 cggcctaaga agggtgggcg agtaaaattc tcgtatgtct aatgccttaa gaaaaatgac
     6421 caactatctc ggtctcaccg aagatgatgg aactagcgaa gttgcaacac ctaaaaacag
     6481 agttgcccca gttaagcgtg agaacaaatc actcactgtc gctccatcta tgactagcgc
     6541 tgcaaaaagc gtagatgctg ctccaattga tcgcatcgtg actattaccc ctaggtttta
     6601 tagtgaggct cgagcaattg gtgagtatta ccgcgaagga aatccggtaa tcatgaacct
     6661 tagcgatatg gaagagtcag agcgcaagag gttaattgac tttgcatcag gtttagtttt
     6721 cggtcatgct ggaactattg agcgcgtgac ttcaaaggtc tttcttctct cgccaccaaa
     6781 tgtgatggtc agttcagaag ataagacggc agcagccaac gctagcctct ttaaccagag
     6841 ctgatttaat tctgctgtga taaatatcag cagttggatt gttcttgctt taaatctctt
     6901 tctctttgcg ttatttgctc gtttaattct ggattatgtc aggatgtttc gtcctggttg
     6961 gcgtccaagg ggaataattc ttccagttgc tgaaatcatc tacatgatta ctgataaacc
     7021 gctcggtttt attcgcagat ttatcccgcc tcttcgcatg ggacctgtgg cacttgatct
     7081 ctcatttatt gtgctcttct ttggaacaca aattcttatc gctttcattc gataaaactt
     7141 ccccctttaa gggcaacttc tcaagaacct aactcctgac ctagactcaa ggttatggaa
     7201 cgcagccaag cgcaagcttt agatgctcaa gatgagttag gtaagtttcg aaatcagttt
     7261 gtcattactg atccaaatac ttgttatcta gatggtaatt ctctgggtcg tcttcctcat
     7321 gccacagtca aagccattag cgactttctc acccaagagt gggggccaaa agttgttacc
     7381 ggctgggagc attggatcga tgaagctcaa acggttggag atttgattgg tagaagcgct
     7441 cttggagcag ggccgggtca agtactggcc tgcgatacaa cttcagtaaa tctctatcaa
     7501 ttagcgctag cagctattaa ggcaaggccg aatagaaaaa cgataataat tgatgcagct
     7561 aatttcccaa ccgatcgtta catcatgcaa ggaattgcaa agagtttagg attaaacctg
     7621 atcacaatta ataatgaaga tagcgcaatt agtgaaaatg aattgataac accagagtta
     7681 cttaaatctt atctatcaga tgatgtggcc ctagtttcac ttcaagttgt gcaatataga
     7741 tcaggtgcga gacaggatat aaaaactctt actgatttag taaagagtca tggagctttg
     7801 atgctttggg atgcagctca cgcggtgggc tcagtagtcc ttgactttga tagaaatggt
     7861 gtagatcttg cagttgggtg cacctacaaa tatggaaact caggaccagg cgctcctgcc
     7921 tggctctatg tttcaaaagg aatccagaaa gagctgcaag ttccaattca aggctggttt
     7981 gcccagcgag atcaatttgc tatgggtgca aattttgaga gagttgaaag tattagaggt
     8041 ttccaaatag caagtccttc acttatgggt ttgagatgca taaagagcgc atttgcgatg
     8101 attgaagagg ctggaattac tgcaatcgaa gcaaaagcag ctagcggaac tcaaatgatg
     8161 attgagctct atgatgagtg gttagcgcct cttggattta ccttaaatac gccaaagact
     8221 gcaagcaaac gcggcggcca tatttctctc tgtcatccag aggcaagcaa aatcgcaata
     8281 gcgcttcgca gatatgccaa tgtaattcca gactatcgcg ctccaaattc aattagagtt
     8341 gcaatctcgc ctttggttaa ctcctatgtt gaggtttatg acggatttga aagaattagg
     8401 gatttagtca gtacccgcca atatgaaaag attgatgaag ggcaggttcg agtcacatga
     8461 gtgaaaatca cgatgctgca ctgacctata gctcttactt aaaagtagat gaacttctaa
     8521 aacttcagca acctctctca gaaggcccag agcatgatga gttgttattc attgtcattc
     8581 atcaaaccta tgaactctgg tttaaagaat tgattcacga atttcttgcc gctcagagaa
     8641 gtcttgaatc gggagatacc cattactcac tctccattct tggtcgaatc aggaccatta
     8701 tgaagatctg tgttgctcag atagacattc ttgagacgat gaccccactt caatttaatt
     8761 cattccgtga tcgccttggg gcatccagcg gctttcaatc ggcgcagttt cggcaaattg
     8821 aagcaattct tggaagacgt gatccaaagg ctgcagcaca cttactacct gcagataaga
     8881 agttcgttga agatttgatg agctcgccat cgctctggga tagcgcactt agatatgtag
     8941 atagcagagg tttcaaaatt gcaacagaaa tactgaatcg agattttagc ttggcttatc
     9001 aagcaaacga aaaagtacaa gaggcgctcc ttgatcttca tagaaaagat cctgaaggct
     9061 ctcttatctg tgagcgttta gttgatatcg atgagggctt gcaagagtgg cgctaccgcc
     9121 atgtaaaaat ggttgagaga acgattggac ataagagtgg aactggtggt tcaagtggcg
     9181 caacatatct tgcttcaact ttatttaatc cagtatttcc tgatctctgg cagattagaa
     9241 gtaagttcta ggcctttgta agtttgaggc taataccgag ctcttgctca cttggcgtta
     9301 gctttgaatc tcgctcaaat gagagagcaa ggacttcctc gcttatccag gcagctccag
     9361 cttcgattgc ctttgaagat tgctcatcag agttccactt aacatggatg cgatcactca
     9421 catcaaaacc ggatgctttt cgctcatctt ggatggcacg aattgcctct ctgactactc
     9481 cctttgcaat taactcatcc gttagattta aatccagcgc aacgctctct ccagtgtgtg
     9541 aggccacagc ccacccagtt cttggtgttt cagtaattac caggtcctcc tcggtaattt
     9601 ctgcagtttt tgagccataa gttatttgag ctttgccact atttcttacc ttacttacta
     9661 attcttgggc gtttgatgag gtaatcgctt ttgatagctc ttgcacctcg ccaccaaatt
     9721 ttgctccaag agatctgaag ttggctttaa tggaaatgtc caccaaatct gctccagcgc
     9781 ttgcaagatc atctagggat tcaacattta attcatctga gatctgttct cgcagttcaa
     9841 cagagagcaa cttccaccca ggtgcggcga taagtgctcg tgatagtggt tgtcgaatct
     9901 tgactccaga ttgagctctt gatgctcgtc caagctctac taatcttcta gtcaaggcaa
     9961 ctgactttga tagttcctta tcaattaatg atgcttcact aaccggaaag tctgctagat
    10021 gaaccgagat cggaccatct ttttcaacta cccgaattaa tttctgccag acatgttcgg
    10081 tgataaatgg aaccatcggt gccataagaa gcgtgacatt tttaaggcag tgatgcagcg
    10141 tcgaaagagc tgcgctatcg ccatcccaga atcttcttct tgatcttctt acataccaat
    10201 ttgataagtc atcgataaat gtggcaatgg cagatcccgc taattgcgaa tcaaagtcac
    10261 ttagagcttg atctaccgtt gcaatcaatt gattcagttc acttaaaatc caacgatcca
    10321 tggttgaaag attgctggcc ttggtaatag gtgttggtga gtaattagca gctgaggcat
    10381 agagcgttaa aaaggagact gtgttccaat aggtaagtag cgtctttctg acaacttcag
    10441 agattgcatc atggccaact cgtctggcgc tccagggaga gccagctgcg agcatgtacc
    10501 aacgaactgc atctgctcca tgtttatcca taagagccat tggctctaat acatttccta
    10561 gatgcttact catcttgcgt ccatctttat ccaagatgtg gccaaggcag agcaccgttt
    10621 tatatgaaga tttatcaaat actaaagttc caattgccat caaggtatag aaccaaccgc
    10681 gggtttgatc aattgcctcg cagataaaat cagccgggta ggcctctttg aatttttctt
    10741 ctgagccttg cttatgagga tatccccact gcgcaaaggg catagcacct gagtcatacc
    10801 agcaatcaat aacttctgga gttctcacca tctgcgatga gcaagtaaga cagttaaata
    10861 caatctcatc aacaaatgga cgatggagtt caagatctgt taaatctttt ccataacggc
    10921 cctgcaattg agctttcgaa gcaaatgctt cctcatgttt attctcgcat ctccagattg
    10981 gaaggggagt gccccaatag cgatttcgcg agagagccca atcgatattg ttattgagcc
    11041 aatcaccata gcgaccattt ttaattgtct caggatgcca attagttgag gcattctctc
    11101 gaagtaaagc atccttgatc ttggtagttc tgatatacca agaaggttgc gcgtaataga
    11161 taagaggagt gtggcatctc cagcaatgcg gataggagtg ttcatattgc tggtgcttat
    11221 aaagtgatcc agatttcttc agctctttaa ttaggatctt atctgcctgc ttaaaaaaca
    11281 ttcctccaac agttgcaatt cctgattcaa attcgccatt gggtgcgatg ggatttacaa
    11341 ccggcaggcc atatttgcgg caggtttgta agtcatctgc gccaaaggct ggggcttgat
    11401 ggactaatcc agttccatct tcggtggtta cataatctgc cagcaccaca tagtgcgcat
    11461 caggaatatc gacataatta aagggcggtt gatatttaac tttctcaagt tggcttccct
    11521 ttaattctcg aagaatttca taactgctac ctaaaatctc aaccaggttc tttgcaacga
    11581 ccaatacttc agagataact tctttttcag gatcagcctt agttctaact accaagtaat
    11641 caacatcggg gttcactgca attgcagtat ttgataccaa ggtccagggt gttgtagtcc
    11701 aaactaatag cgatgcaccg agattcttta actcaccatc aataattgga aaacgcagat
    11761 acacactcgg atcagtaacg ctttcatagc cttgggccag ctcatgatcc gataggccag
    11821 tgccacatct agggcaatac ggcgctacgc gatagtcctg aaccagtaaa cctttgctat
    11881 gaatctgagc aagtgaccac cagacacttt caatatattc attagacata gtccaatagg
    11941 cctgatcaaa atcaacccag aaacccattc gctcggtcat ctcagtaaat gcgccaacat
    12001 gtcgctgcac cgattgacga cacttttcat taaaggcagc tattccaaat ttttcgatat
    12061 caccttttcc agaaaatccc agttccttct caactgcaat ctctactgga agaccatggc
    12121 aatcccaacc tgctttgcgg attacatact tgcccttcat cgtctgaaag cgaggaaata
    12181 catccttaaa gactcttgcc tcaatatggt gagttcctgg cagaccattt gcagttggcg
    12241 gtccttcata aaaagtccaa cttggaccac cttgacgcat tctgacgctg ctgtgaaaaa
    12301 tgtcttgggt ttgccagaaa tctaaaatct catgttcaac ttttggaaga tcaatcgagg
    12361 cagatagcgc tagataaggc gaattacttg acactaaaaa ccccctcgaa attcgaataa
    12421 aacgctcttc gatctcttgg aggggcgatc ttgcgaccgc ggtaccaccc gccttgcagc
    12481 ttatttgctg ccgcttttaa aaaacttact gctggttcta gatgagctct cgactcattt
    12541 cttccaactc gctcacaggt gattgcctgg tcatcgcgat aactgatgta agcgcgctca
    12601 ttctagccta tagcggggcg agtggcattt aaatcaataa acctttagcc ttcgccgttg
    12661 tggcattgaa aaagaaagtc cgctcagcgg ttgcaaagtt taagaagaaa tctagcccag
    12721 caaaggcaaa acctaaggcc aagagcgtta agccaaaggc taaaaaagct cctgtaaaaa
    12781 aagttacagc caaaaaagct ccagcaaaaa aagtgccagt aaagaaagca ccagttcgcc
    12841 caactcttgc taaaaaagct ccattaaaga aggcccctgc tcagattatg aagggcgctc
    12901 ataagaccgc gctcaccagt agtactgcaa aaggcggagc gacaactatt tcagtttata
    12961 aaccagagat cgaagcaagt gatcttgtag ttgttccaga aaaacgttta gtgatgccgc
    13021 cgccaattag aactccaaag ccaggcaaga aagctcctgc gctaaagcca gttaaggcag
    13081 cgccatcacc ctttgttcta gatggcgagg agtcttggag cgcagctgaa atgaaggcaa
    13141 tcaaatctga actttcaaaa gatttaattc gattaaagaa agaacttgaa accattgaaa
    13201 gtgagatgga tgatctaatt gaagcttcag gtgtcggagc aggagatgat caagctgatg
    13261 ctggcgccaa aacatttgaa cgtgaacatg aaatctcact ggtctataac gcaagagata
    13321 tggttttaca gaccgagaga gcgttagagc gaattgagac taaaacttat gggcgatgcg
    13381 aggattgtgg aagtgcgatt ggaaaagccc gtctccaagt tttccctcgc gccactcttt
    13441 gcatgttatg caaacagaag gaagagcgcc gataaggtcg tcgggtgcta atcgcgcgcg
    13501 acgactcttt gtaatattta ccgtatcaat atttgctctg gatttaatta ctaaaaactg
    13561 ggccatttca tatctgcaat accgcgaacc ggttaaggtt ataggagact ttctgcaatt
    13621 aacttttgcc acaaacccag gtgctgcttt taatttaatt ggagatgcca ccttaatttt
    13681 gagctctctt aaactttgtg tcgccgcctt aatcctttat ttcattagaa aagtaacgaa
    13741 tggatcctgg gcgattgctc tgggtctatt gcttggagga gttcttggaa acttgatgga
    13801 tcgagccttt agacctcctg ggccatggcg aggtgaggtt attgattgga tcgagctacc
    13861 taattggcca gtatttaaca tagctgattc agcaattgtt acttcagctc tctttatgag
    13921 ccttttagct attagaaata ttgaaccata taagaaggtc atctaatgga gcgtgagaag
    13981 agagcgattc agattgatca agatcaagat ggtcagagag ttgatacagc tttagcaaaa
    14041 gttctagagc tatcaagaag tgtggtggct gatctactta atgccggtga tgtgctgcag
    14101 ggaaagaaac cgctatcaaa gtcagatcgg gtaagcgcag gagatcgctt aacggtgtta
    14161 atgccagcta tttatgatcc tctggagttg aaagagacgc cgatagatac ccttgaaatt
    14221 atttatgacg atgatgatgt cgttgtagtt aataaaccag tgggttgcgc tgcgcatgca
    14281 agtccaggct ggatgggccc aactgttgtt ggtgcgcttc ttgccagagg ttatcgaatc
    14341 tcaacaactg gaccacaaga gcgacaggga attgtgcaaa ggcttgatgc tggaacttct
    14401 ggactaatgc tgttagcaaa gcatgagcgt tcctatattt ctatgaagaa ccaatttaga
    14461 aatcgcagta tcgaaaaggt ttatcgcacc ctcattcaag gacatattga tccagctgag
    14521 ggaagtattg atgctcctat cgggcgccat ccaagagagg attatcgttt tgctgttgtt
    14581 gcagatggca aggccagcat tactcactat gaattaattg agtactacca aggggcatct
    14641 ctattgaagg tagttttaga aacgggaaga acgcatcaga taagagttca cttcaatgcc
    14701 ctgcgccatc cgctagttgg cgatcttgct tacggtgggg acccagtcct tgccgcacgt
    14761 ctggagttaa agagaccttg gctgcatgcg ctggagctat cttttaatca accatctagt
    14821 gataagcgaa tcaccctaaa tgccccactg cctgaggacc taacgcgcgc ccttgcgttg
    14881 ctcgccgtca agtgagtgca agagagtaat ctgcccgacc gtgagccaag gttttgtcca
    14941 tctacatgtc catactgagt attcgatgct cgatggagct gctcgcattg aaaaattagc
    15001 cgaggaagtc gctcgctctg gtgatggcgc aattgcaatg accgatcatg gaaatgtatt
    15061 cggcgcattt gatttctata aaacaatgaa taacgctgga gttaaaccca tcattggaat
    15121 agaggcttat gtagctccgg aatctaggtt tgataagaag cgagttaaat gggctgaggg
    15181 cggggaagat gatgtctcag caggcggggc atatacccat atgacgctct tggctgaaaa
    15241 taatgttggc cttgctaatt tatttcgtct ttcatctcta gcatcgctgg aaggttttta
    15301 ttacaaacca agaatggatc gcgagttgct ctcgcaatat agccaaggaa ttattgcaac
    15361 aacgggatgt gcggcaggtg agattcaaac tcgcatcaga atgggggcct acaacgaagc
    15421 ccgggcagcg gcagcaaatt accgcgatat tttcggagca gataattact ttgttgaaat
    15481 tatggatcac gacattgaaa ttgaaagacg cgttaaagat gatttgctaa agctcgcaaa
    15541 agagttaggg cttccacttg ttgcaacaaa cgatcttcac tacacatatg cagatgatgc
    15601 aaaacatcat gaggctcttc tctgtatcca atccggcaca actctggcag atccaaagag
    15661 atttagattt gaaggtaaag agtattacct aaaagatgca gcaacgatgc gtcgtatctt
    15721 taaagatctt gaaattgcct gtgataacac ccttgcaatt gctgaaagat gcaatataaa
    15781 gatgcgtgaa aatgagaatt tgctcccaca atttgcagtg ccaaaaggtg agagtgagaa
    15841 ctcctggtta gtcaaagagg cagaagctgg attattggaa aaatttggag caaagagtat
    15901 ggatgatatt gctccgcaat atcaggccag acttcacttt gaattagatg tgatgaccaa
    15961 aatgggattt gcaggttact tcttagttgt tgcagatctt gtagctcacg caaagagaga
    16021 gggtattcgt gttgggccgg gaagaggatc ggctgcagga tctttagttt catatgcttt
    16081 aggaattacc ggccttgatc caattaagca tggattgctc tttgagcgtt ttcttaatcc
    16141 agaccgtatc tccatgcctg atatcgactt ggactttgat gagcgtaggc gctccgagat
    16201 gatcaggtat gccaccaata aatatggtga tgatcgagtg gcccaaatca tcacttactc
    16261 aactattaaa tctaaagctg cgataaaaga tgctgctcga gttttaggtt atccctacgc
    16321 tttaggtgag cgcttaacca aggcgctacc accgccaatt atgggaaaag atgtttcatt
    16381 tcacggcgtt tttgataagg acgatgagcg ctacggcgag gcacaagaat ttagaaatat
    16441 atatgagagt gatcctgact ctaaaactat tgtagatatg gcaagagggc tagaaggact
    16501 gaagcgacag tggtcagttc atgctgcagg agtgattctt agtagagaac ctttgattga
    16561 tgtgattcca attcacaaaa gagatgtgga tggctcgatt atcactcagt ttgatatggg
    16621 tgcctgcgaa gcaacagggc tgttaaaaat ggactttctc ggacttcgaa atctctcagt
    16681 tcttgatgac tgcctagaaa atattaagaa caatcaggca aaaacaattg ttctagaatc
    16741 cttagagttg tcagataaga aaacctttga attactctca agaggtgaga ctctaggggt
    16801 atttcaatta gatagcgctc cgattcgagc cctgcttaga tcaatggcgc cagattcatt
    16861 tgaagacatt tcagctgtta ttgcgctata tcgccccgga cctatgggag ttaacgctca
    16921 taatgattac gcagatcgca aaaataaacg taagagaatt gagccaattc atcctgagtt
    16981 aagtgccgca cttgatgaga tattggcaga tacctatgga cttattgttt atcaagaaca
    17041 ggttatggca attgcacaaa agcttgctgg tttttctcta ggacgagcag atctactacg
    17101 taaagctatg ggtaagaaga ataaagagat tctggataaa gagtatgttc actttgaaga
    17161 gggtatgcgt aataatggat tttcaagtat tgctatcgaa gagctttgga agacactgat
    17221 tccattctca gattacgcct ttaaccgggc acatagcgca ggctatggcg tcctatcatt
    17281 ttggaccgct tacctaaagg cgaattatcc aactgaatat atggcagctc ttcttaccag
    17341 cgttagagat gataaagata agtctgcgct ttacttaaat gaatctcgcc gaatgggaat
    17401 taaagttctg cctcctgatg tgaatttctc aaatgctgag tacaccccag gtggtagcga
    17461 cattaggttt ggcctaactg ccattagaaa tattggggaa aatgttgtgg catcgatagt
    17521 aaagaatcgc aacgagaagg gtaactacac ctcctttagc gattttctag ctaaagtcga
    17581 tgggacggtc tgtaataaaa aaactataga gtcattaatt aaagccgggg cctttgattc
    17641 actggggcat tcgcgcaagg ggctaatgag tatttatcta gaagcaattg attctgtgat
    17701 ggaagaaaag cgcgcccagg ctattggtca atttgattta tttggcgggg caaatgaagt
    17761 gagcgcaaat ggcatttcaa ctctggcgct agaagttagt gatcaggagt gggataaggc
    17821 gctgctactt tcctacgaga gagaaatgct gggtctatat gtttcagatc atccacttct
    17881 tggagtggag catgttttaa ggagcgcaac tgatacaaca attagccaga ttctagatga
    17941 aggggtagtt cacgatcaga taatcactat tgcaggtcta atcacagcgg ttcaacgaaa
    18001 agttagtcgg caaggacaat cttgggcaat tgtcacagtt gaagatcttg aaggcgctat
    18061 cgatgtgcgc ttcttctcat ctacctatca aacccatgct ctaaatttga ttgaagatcg
    18121 aatcgtaatt attcgcggaa gaattgataa acgtgaggag acagcgcaga ttacggctct
    18181 tgagctaaat cttgccgaca taaatcaagc accaataggt ccctttgtaa ttaaacttga
    18241 agccgagcga tgcacacctc ctgttgtaga tcgaatgaag gagatattgc gctcccatcc
    18301 tggaactaga gaagttcatc taaaaattga aggcggcgca aaaaacacaa ttcttaagct
    18361 cgatgatggc ttgaaaatta ctgcttcacc aagcttgagc gcagatctca agtcaattct
    18421 tggcccagac tgtttagttt aagcagagat aactctctgc aagattagag gcagggcgct
    18481 tagaatagtg cgaatggaaa aattggagtt actacctgcc cttgatattc gtgccggcga
    18541 ggcagtccga cttattcaag gtgatctgag tcagcaggct aagtatggat ctgccctaga
    18601 tgtagcactt gaatttcaaa gtgctggggc agagtggatt cacttagttg atttagatgc
    18661 agcttttcgc actggctcca attatgatct tttagtagaa gttgttggaa aactagatgt
    18721 aaaggtccag ttatcaggtg gaattcgaga tcaagagtct ttggagcgcg ctcttgcgac
    18781 gggatgccac cgagttaatc tctctacttc agcgcttctg gatttgtcat ggtgtgaatc
    18841 tgtaatcaaa cggtttggcg aaaaagttgc cgtaggactc gatgtaacag gaagaactct
    18901 ggcggcgcga ggggacagtc aagaggtcgg cgacttattt gatgttctaa agcaattaga
    18961 tggctacggt agttctagat atgtattaac tgatgtgagt agggatggaa cgcttgaggg
    19021 gccaaaccta gatttgatca aatcagtttg tgcagaaaca gatagaccag taattgcatc
    19081 tggaggaatt tcgcagttaa gcgacattca atctctccga gagctgatag caataggggt
    19141 agaaggggca attatcggca aggctttgta tgcccagaac ttcactattg gacaagctct
    19201 agaagtagca gcaaaatgaa tgctatgaga ctgactcaaa ttgaagcagt caaaaatgca
    19261 gaaggtaaat aaatggagcg aatgaacctt gaacaatttc gctcctatgc gaaggaatat
    19321 aatgtaattc ctgtttctca agtactgctt gcagataatg aaactccact gactctatat
    19381 tcaaagttag ttcaaaatcg agaaaataca tttctcttag aatcagcaga gcatggtgga
    19441 gcatggtcta gatactcttt tattggcgtt agaagtgaag ctaccctaac cgagaaaaac
    19501 ggagttgctc attggcttgg aagtatgcca gcaggtgcgc caaatggaat cgatccctta
    19561 gaagctctaa tgattgcctc taaacatctt cgatcgccaa agattgaaaa tctcccacca
    19621 ttaacaggtg gcttagtcgg ctatatggga tatgacgcgg tgcgaaggct ggagaaactt
    19681 ccaaatttag gaattgatga attaaagctc ccagagattg catttatgtt gactacagat
    19741 ctagctgttt atgatcatct tcaaggaact gtgattctga tagctaatgc tattaattgg
    19801 gatggatccg atgaacgggt agatcttgca tatcaatcgg ctataaatcg attagatcaa
    19861 atgcaggagg cacttctgaa taagatagat agcaatattt ctaagatagc aaagaagtca
    19921 gcgccaagct ttactgctaa tttcactaaa gatcaattct gtaaaagcgt ggaaagcatc
    19981 aaagaacaga ttagatctgg cgaggcattc caagttgttt tatctcagag atttacgatg
    20041 gattgccaag catcctcact tgatgcatac cgaatgctga ggctccacaa tcccagccca
    20101 tatatgtatc acttcagatt tgcagatggg ttaagtgttg ttggctcaag tcctgaggct
    20161 ttggttaaag taactggcag tgaagtaatg gttcatccca ttgctggaac tcgaaaacgt
    20221 tcaacaaatc aagaagaaga cttaaggcta ggcgaggagc ttttggcaga tcctaaagag
    20281 cgcgctgagc atctaatgct ggtagatctt ggaagaaatg atttgggaag agtttgcgca
    20341 cctggcagcg taaatgttgt tgagtttatg cagattgaga gatattcaca tgtcatgcat
    20401 attgtctcaa ctgttattgg cgagctagat agcgctaagt cacctgtgga ggctttatat
    20461 gcggtatttc ctgccggcac tctctcagga gcacctaagc ccagagcgat ggagataatt
    20521 gaagagttag aaccatcaag acgaggaatc tacggtggcg caattggata tatagatttc
    20581 actggaaatc ttgacacttg tattgctatc agaactgcag tgataaaaga taagaaagca
    20641 tatgtccaag caggagctgg agtagtggca gattcaaacc cagttagtga atatgaggag
    20701 tgcctcaata aagccgcggc ggtgcttggc gcaattgaag cggcaaactc actagaacca
    20761 cttggagaaa aggactagct gaaatgagcg ttcttaattc aataatcgct ggtgttttag
    20821 aagatcaagc taggcgccaa ctatctaact ctgagctaga ggagaaaatg gcattagctg
    20881 ctaagcccct tgatgttttg gcagctctta gcagagagaa attttcaatc attgcagaag
    20941 ttaagcgatc aagccctagt aaagggtccc ttgccgaaat accttctcca gaaaaacttg
    21001 caagacatta tcaagaaggc ggagcagcag ttattagcgt cttaactgag cagagacgct
    21061 ttggtggttc gcttgaagat tttgaaaaag ttagaagcga agtcacaatt ccacttctac
    21121 gaaaagactt tatagttaat gagtatctag tcaaagaatc aagggcctat ggtgcagatc
    21181 tgattcttct aattgttgca gcgctaggtg atgatgaatt aaaacagctc tatcaattag
    21241 taaaaagtct cggcatggag gcgttaattg agattcacaa tcaagaagag cttgagcggg
    21301 cacttgctat aggcccaaaa ataattggag taaatgcacg aaatctaaag actttagaga
    21361 ttgatcagag taacttcgca aaactactac ctctgattcc atccggaatc gttcgtattg
    21421 ctgagtcagg tatgtctgag ccaagtgatg ggcaaagtgc tttgattgct ggagcagatg
    21481 cgatcttggt aggagaggct ctagttaaat cagaaaatcc tgccgatagc gttcgtgaat
    21541 tcttaaatgt tgcaaactct tgaaggaaag tagactaaag ggtatggagc gcgttgaagt
    21601 tgttggccac tttggccctt atggtgggcg ctttgttcca gaggctctaa ttggcgccct
    21661 cgatgaatta gaggcagctc ataactcggc ttctaaagat cctgaatttc aaagagaact
    21721 aaaccatttg cataagagct ataccggtcg gccatcaata attactgagg ctaagagatt
    21781 tgctgaacat gctggaaatg caaggattct tttaaagcgt gaagatttga atcacactgg
    21841 gagccataag attaataatg ttcttggtca agcacttctc accaaacgaa tgggtaagaa
    21901 gcgaataatc gctgaaactg gagcgggcca acatggtgtt gcttcagcaa ctgcagcagc
    21961 cctgcttggt cttgagtgtg tggtttatat gggcgaggaa gatacaaaac gccaatctct
    22021 aaacgttgcc agaatgaaat tacttggcgc aacagtaatt ccagtgacaa ctggctctag
    22081 aactctaaaa gatgctatta acgaagcgat gcgtgattgg gttaccaatg tttcaactac
    22141 tcactaccta ttaggaactg ttgctggccc acatcctttc ccaactatgg tgagagattt
    22201 tcatcgcata attggaattg aagcaaggca gcaagtatta gagatgaccg gaaggcttcc
    22261 agatgcagtt ttagcttgtg ttggcggagg atctaatgca ataggaatat tccatccttt
    22321 tattgatgat gcgggagtgc ggttgattgg gttagaagct ggtggtgccg gagttgaaag
    22381 tggtaaacac gctgcaacta ttgtgggtgg gacacctgga gttcttcatg gcaccaggtc
    22441 ttatctactt caagatagtg atgggcaaac catcgagtct cattcaattt ctgctggcct
    22501 tgactatcca ggagtaggcc ctgagcatgc ctacttgcat gacattggac gagctgaata
    22561 tcgagcaatt accgatgatc aggcgatgca tgccttttca ctcctttcaa agagtgaggg
    22621 aataattccc gcaattgaga ctgcgcacgc actagctgga gcgctgcaag taggaaatga
    22681 actgggaagt ggcgcaatcc ttctaattaa tctctcagga cgcggcgata aagatgttca
    22741 aacagctgcg cagtactttg gaattccact ctaggaatat tgcaatcaat gagtaaagag
    22801 agtttggcgc aagtctttgc acgagctaag agcgaaaata gagcagccct tatcggttat
    22861 atgccagcgg gctttccaac ccttgaagga tccaaagaat taataagggc gctaattgct
    22921 ggtggagtgg atatcatcga agtgggtttt gcatatagcg atccagttat ggatggagcg
    22981 ataattcaag aagctgctga gattgctcta agaaataaaa ctggtgctaa agaagtattg
    23041 gaagttgttt ctggcgtgag tgttccatct ttagttatgt cctattggaa tcccattgag
    23101 cgatttggtg tcaaggaatt cacaaaggct cttgctagtg cggggggagt tggagtcata
    23161 actccagatc taactattga agaatcaggt gattggattg ttgagaccga gagaaatgat
    23221 ttgcaacgtg tatatgttgt tgctcccagc acagatgatt caagattagc taaagttgca
    23281 gataagtgct ctggatttgt ttatgcggca agtcttatgg gagtaactgg agcaagaaat
    23341 tctctctcag atagtgccag tgaattagtt gcaagacttc gcaaggttac aaatcttcca
    23401 atcgcggtgg gccttggagt ttcaactgca gagcaggctg cagatgttgc aaagtttgcc
    23461 gatggggtca ttgtgggctc agcatttatt aagttagtac tacaggctcc tgatttcacc
    23521 caggcatgca aaaacgttac cttgctagcc cgcgagttgt ctgatggggt cagacagggc
    23581 cgagtcggct agtttctaga acttggttta gacttatagc catacgcagt taggaaaatc
    23641 tagaaggagt aggaatagtg ccaaaagaac cgcgccaaag aaaagtaagt aactctgcta
    23701 aatctggaga tactgtcact agaaatatcg tgattggcat gattgcactt gtagtcttat
    23761 ctggagttat cttcactgtt ttggataaga gatctggttc tagcgttgca cttcctgcat
    23821 cgattgagag catctcaagt gctaacaatg gtgagcctct aactccaacg atttctccag
    23881 aggcagatta tggtgtggtc tttaacgccg atagcccact aaaaattgac atctgggaag
    23941 attttcaatg cccatattgc aagttctttg aagatgcgat gggtgactac ttagagactt
    24001 taattagaga gaagcaagca gaagttacct accacatggc atcatttcta ggacaagagt
    24061 cagttcgcgc ctccaatgct gcaaattgcg cagtacctga aggaagattt attgagtatc
    24121 acagagcgct ctttgatatc caaggtggag aaaacagtgg gctattttct aacaagaatc
    24181 tagttgaaat tggcacgcgc cttggaatca ctagtgagag ctttgctaac tgcgtcaata
    24241 acaatgaatc aggtgatgta gtcaaaaacg ttgcatcttc catgaagaaa aatggcgtcg
    24301 aaggaactcc tactgtattt atcaacggca agccgtggaa tcgcaccacc tctgaattta
    24361 gattagagga gtttaaggcg gcggtcgaag cagctaagca gtgatctttg ctgctatccc
    24421 ttctccaact caatctcaaa tagagattgg tccactaact tttcacttct atgccttaag
    24481 cataattaca ggaattattg ctgctgtttt tattggaaat cggagatatg ttgcacttgg
    24541 tggcagagca ggggtagtta gcgatgttgc aatctatgca gttccatttg ggattattgg
    24601 tggtcgcctc tatcacgtaa tttcttcacc gcaggcatat ttcggaacaa atggtgatcc
    24661 gctaagtgcg ctctacatct ggcaaggtgg acttggaatc tggggagcaa tttcccttgg
    24721 acttcttggc gcatatattg gatatcgcag gaataaatct cgaggtgatg tttcctttgc
    24781 aagctttgct gatgcgcttg ctccggggtt attgattgct caaggcattg gacgttgggg
    24841 aaattggttt aataaagaac tctttggacg agagctaaat gctccatggg cccttgaaat
    24901 tccagcctcc tatcgcccga ttggatattc aagcgttgaa acatttcatc cggtattttt
    24961 atatgaatcg atctggtgct ttatcgcagc tctaattttg atcaaagtgg gatcaaggaa
    25021 gaggtttgca ccgggttcaa tttttaccct ctatgtcgcc ctttattgcc tagggagagc
    25081 tctgattgaa acgattagaa tcgatgaagc aaatctaatt tttggcgtca ggctcaatgt
    25141 ttggaccagc ggcgttctac tctttgcctc tcttatctac ttgcgtcaga atcaattacc
    25201 gaaagaatcg agtgtaaagt aacctcgtga gccaatctga tttagagata agaaattctg
    25261 agcatcgcac tcatagatca ggatggttga gagctgccgt attaggtgtt aatgatgggt
    25321 tagtttcaac tgcagcatta atgattggaa ttgtttcggc acaacaaaat gactttgtag
    25381 taactgttgg tatggcagga attgcagctg gagcgatgtc aatggcggtt ggcgaatatg
    25441 tctcggttag gtcgcagaac gatattgaag aatcggatcg aatcttggag ctagagcacc
    25501 atcgaattga tccagcggga gagttagaag aattaactca aatttatcaa tctagaggtc
    25561 tatctagagc aacagcggag aaagtagcgc aagaacttca tgcccatgac gcactgggtg
    25621 ctcacctgcg agatgagttg ggccaatatg aattaactaa ggctcgtcca atgcaggcag
    25681 caattgcctc tgcacttagc ttcacactcg ggggagctat tcctcttatt ggagctcttg
    25741 ccccaagtct tggaggaaaa gctgtttcaa ttgttgcaat tacattcttt ggtctactaa
    25801 ttgccggagt aattagcgca aagacttccg ggtcaaagat atggcgacca acccttcgta
    25861 tcctgcttgg tggcgctctt ggaatggcta ttacggcatc tgtaggtcag attgcccata
    25921 tctctgggct ctagcacgcg ccaaaaaagc tacttcttga tagattaagg ctcttataag
    25981 ggccgtcgtt gtcccagtaa cttcgaaagc aaactaagaa aaaattcgaa gcagtctatt
    26041 ttttatggga gcgataagtg gtcttctcat ctctaccgaa ggcagtaggt ctctatgatc
    26101 ctgctaacga gcatgatgct tgcggtgttg cgatggtggc taccctcaac aaagttgcaa
    26161 ctcatgaaat cattgcaaaa ggtttagttg ccttaagaaa cttagaacat cgaggcgcat
    26221 caggagctga agttaatagt ggtgatggcg ccggaattct tatctgtatt cctgatcaat
    26281 tcttccgcgc agttctgcct tttgagttgc cagcacctct taggtatgca gctggagttg
    26341 ttttcctccc aaagaatttt gcaagtaaat caaggattga gcagattgca agcgaggaga
    26401 atctaataat tctaggttgg cgccagctgc caattaacgc atcatctctt ggagccacag
    26461 ctttatcggt aatgcctgaa tttgagcaga tatttgtttc atcccaggat ggagttagtg
    26521 acttagattt agatcgaaga gcattctgct ttagaaagag agttgagcat gaactctcaa
    26581 tttactttac ttcgctctct agtaggacga ttgtctataa aggaatgctc actacagggc
    26641 aattagagga attctttcca gatctctcag atccgcgttt agtatcacct cttgctctag
    26701 ttcactcgag attttctacc aacacttttc catcttggcc acttgcccat ccctatcgat
    26761 tcatcgctca taatggtgag ataaatacgg ttagaggaaa tcgcaattgg atggcagccc
    26821 gtgaagcact ccttgaaagt gatctgatcc ctggcgatat ctctcgacta tttccaatag
    26881 ttgatccatc tggaagtgat tcggcctcct ttgatgaagt gttagagctt ctttatctgg
    26941 gcgggcgcag ccttgcacat tccattctta tgatgattcc agaggcttgg gaaaatcacg
    27001 aaacaatgcc tgaaaaatta aaagacttct atcgtttcca tgcatcttta atggagccat
    27061 gggatggccc agcctgcgtc actttcacag atggaaagca gattggtgca gtattggatc
    27121 gaaatggatt gcgtccatct cgctactggg tcacagatga tggtttggtg gtgctggcaa
    27181 gtgaagttgg cgtgttagat attccgcaag aaaaagttat tcgcaaggga agacttcaac
    27241 caggtcgcat gttccttgtt gatatccaat ctggtcgcat cattgaagat gaggagatta
    27301 agcagagcct tgcaactaac gctccttatg gcgaatggct ccatgccgga attgtgcgcc
    27361 tctctgattt accagctcga gagcacatca tttatccaca ctcatctgtt ttaagaagac
    27421 agcgcgcatt tggttatacc gaagaagagt taagaatgat tgtaactccg atggcaaaaa
    27481 gtggaattga accaatcgga tctatgggaa ctgatactcc gattgcagct ctatctgcga
    27541 agccacgact cttgtttgat tacttctcgc agctttttgc gcaagtaaca aatccaccac
    27601 ttgatgccat tagggaggag ctagttacct cacttggggg atccattgga ccagaacata
    27661 atcttctaga tccaggccca gcatcgtgtc ggcagatttc tttggctttt ccagttatag
    27721 ataatgatga gcttgcaaaa ataattaacg taaatgctga taacaatcat ccagggctat
    27781 ctacttatgt aatacgcggc ctcttcccaa tttctaaagg cggccaaggc ttgcaagaga
    27841 gaattgagga gattcgagct gaagtctcgg aggcaatctc tcaaggtgct cgaatcattg
    27901 ttctctctga tcgtgatggc gatgcagaga acgcaccgat tccatccctg cttctaacat
    27961 cggcagtgca tcatcaccta attcgcgaga aaacaagaac taaagttgga ttagttgttg
    28021 aaagtggcga ggtaagagaa gttcaccacg ttgcgctttt aattggctac ggagctgctg
    28081 cggtaaatcc atatctagcg atggagagtg ctgaggattt agttttaact ggagtaatta
    28141 caggcataac gcctgagaag gcagtgaaga atttaattaa gtcattgggc aaaggcgttt
    28201 taaaggttat gtccaagatg ggaatatcaa cgattgcctc ctacaccgga gcgcaagttt
    28261 ttgaagctat cggattgtca caagcattag ttaacgaata ctttgttgga acaacttcgc
    28321 gccttggtgg agtagatctc gaagtaatcg cgcaagaaac aattgcgcgc catcacatcg
    28381 cctatccgcc tgggggagaa gttccaggat ctaaacgact tccaattggt ggggagtatc
    28441 aatggcgcag agatggcgaa cctcatctat ttgatccaga gactgtcttt gctctccaac
    28501 atgccaccag atcaaagcgc tatgacatct tccagagata taccaagcga gtagatgatc
    28561 aatccaaatc tttgatgacg cttcgcggtc tcttcacctt taagcaggga ctaagaaaac
    28621 cagtagctat agatgaagtt gagccagttt cagaaattgt taagaggttt tcaactggag
    28681 ctatgtcata tggctcaatc tcacaagaag ctcatgaaac gctagcaatt gctatgaatc
    28741 gaattggcgc taaatcaaat actggagaag gcggcgaaga tcctgagcgt ttcattcctc
    28801 aagccaatgg agattcagca agatctgcaa tcaagcaagt tgcttcagga agatttggtg
    28861 taacaagtga atatctagta aatgccgatg atattcagat aaaaatggca caaggagcta
    28921 aacctggcga gggtgggcaa ttgcctggaa ataaaattta tccctggatt gccaaggtgc
    28981 gctactcaac tcctggcgtt ggcttaatct caccgccgcc acatcatgat atttactcga
    29041 tcgaagattt agcgcaacta attcacgatt taaagaatgc aaataacaga gcgcgtatcc
    29101 atgtaaaact cgtggctgaa gtcggagttg gcactgttgc agcaggagtt agcaaggcgc
    29161 atgccgatgt tgttttaatc tctggccatg atggtggaac tggagcatct ccgctaactt
    29221 ccctcaaaca tgctggcgct ccatgggagc taggtcttgc tgaaacccaa cagactctga
    29281 tgctaaatgg actaagagat cgcatcgttg ttcaaactga tggacagatg aagacaggaa
    29341 gagatgtggt tattgccgcc ctccttggtg ccgaggaatt tggttttgct actgctccac
    29401 ttgtggtttc aggctgtgtg atgatgcgag tctgtcactt agatacctgc ccagtgggtg
    29461 ttgcaactca aaatccagag ctaagaaaac gctttactgg aaagccagaa ttcgttgaaa
    29521 ctttctttga atacatcgct caggaggtaa gagagattct tgcaagccta ggatttagaa
    29581 gtcttagcga agcagtcggc gcagttgaga gtctagaaac ttctgctgca gtggagcatt
    29641 ggaaggcaag tggacttgat ttatcaccaa ttcttgctgc gccagaatca gcgtcaggat
    29701 ctgcgcgtag aaataccacg actcaagatc atgggcttga agctgctcta gataatgagc
    29761 taataaaact ttcacagagc gccttagaaa gtaagtctcc aattaaattg cagatgaaaa
    29821 ttagaaatgg taatcgaaca gttggaacga tgctgggctc tgaaattact agaaaatatg
    29881 gcggtcaagg ccttgccgat gacacaattg atatcacctt ccatggctct gcaggtcaat
    29941 cgctcggagc atttattcca cgcgggctca ctcttcgcct ctatggagat accaatgatt
    30001 atcttggaaa ggggctatct ggcgggcgag taattctgcg ccctgatgag aaggcaaagt
    30061 ttgcctccca tgaaaatgta attgctggaa atgttattgg atatggggca actcaaggtg
    30121 agatatttgt aagaggaata gtgggcgaga gattctgtgt aagaaactct ggagcgctag
    30181 ccgttgttga aggtgttggc gatcatggct gcgaatatat gactggcgga actgtggtca
    30241 ttcttggcaa aacagggcga aactttgcag ctggcatgtc aggtggccga gcatttgttc
    30301 ttgatttaat cccatcgcta gtaaataccg agatggttga tgttttatct atccctaaag
    30361 atcaagagga gaaactaaaa tccatactct ctcgtttcta tgcagagacc ggttctttaa
    30421 ttgcacatgc attacttgga gattggcagg cagcattgaa gagattctca cttgtgatgc
    30481 caagagatta tgccaaggtg ctggcagtaa ttgatagagc agagcgcgaa ggaaaatcat
    30541 ctgatgatgc aattatggag gcgctaaatg gctgatcccc gtggctttct aaatactgga
    30601 agagaagttc caaaacgccg cccagtagat gttcgtatca gagattggcg tgaggtttat
    30661 gaggagcaag agttctcatc ccttcaaaag caagcaggaa ggtgtatgga ttgtggaatt
    30721 cctttctgtc atcagggctg tccattagga aatttaattc cagaatggaa tgacttgatt
    30781 tggcgtaatg aaaaggtaga tgcgctagat cgcctccacg caactaataa ttttcctgaa
    30841 tttactggac gactctgccc tgctccctgc gaaacagctt gcgttctggg tattaatcaa
    30901 gatccagtga ctattaaaca agttgagtta agaattattg aagagggatt tcaaagtgga
    30961 aatgtgaagc cgcttcaacc agatcggatg actggcaaaa ccattgcagt tattggctcg
    31021 ggccctgccg gtcttgccgc agcccagcaa ctaacaagag caggtcatac tgttgtggtc
    31081 tatgaaaaag gtgagaaagt tggaggatta ctccgctatg gcattccaga attcaaaatg
    31141 gagaaagcga ttcttgatcg tcgtataaac cagatgcagc gcgaagggac aagatttaga
    31201 gcaggagtta acgttggagt tgatatcagc gctgcagagc ttcgcgctaa atatgacgca
    31261 atagttttag ctgtaggggc gactcaatgg cgtgaactcg atatcccggg aagatcactt
    31321 aatggtgttt atcaagcgat ggaatatctg ccatggggaa acaagcaagc cctcaatgaa
    31381 ttaccttctg agccaccgat taatgccaag ggtaaaaatg taatcatttt gggaggtggc
    31441 gatactggcg ctgattgtct aggaactgca atccgtcaag gagctgcaag tgttactcaa
    31501 attgagatta tgccaaggcc aagtgatgag cgccctgact cacagccatg gccaacttat
    31561 ccaatgactt atcgtgtaag tagcgcgcac gaagaatcag gcgagcgact ctattcaatt
    31621 tcaactgaag aatttattgg caatagcgct gggcaattga ctgccttaaa aattgttgag
    31681 acgaagtatg ttgatggaaa gtttgaaaaa gttccaggaa cttcaaggga tcttcctgca
    31741 gatatggtgt tactagccat gggctttact ggacctgaga agaatgaatt agtaaaagcg
    31801 ctcgaattga aacttgattc tcgcggaaat attgctcgcg acaattcata tcgcaccaac
    31861 gttgactcgg tctatatctg cggtgatgca ggacgtggtc aatcactaat tgtttgggcg
    31921 attgcagagg ggagaagcgc tgcttccgca gtagatgcag atctaatggg acatacgcag
    31981 ttgccatcgc cgattgaacc aactgctcga ccactaacgg tttaattccg tgctaattac
    32041 tcttatgcca aataaggcaa taaagtaagc acatgcgccg cgccaaaatt gtctgcacca
    32101 tgggccctgc agttgagagc gttgaaaaag tctcggagct aattcgagct ggaatgaatg
    32161 tggcaaggct taatctctcc catggtggat atgaagagca tcaaaaccgc ctagatttag
    32221 taagggctga ggctgcgcga gttaaacagc ctgtggcaat catggttgat cttcaaggac
    32281 caaagattcg cctaggaaga tttgaaaacg gccctcatga attaatgcgt ggagatattt
    32341 ttaccattac taccgatgac attactggcg acaagtctcg cgtctcaact acctataaag
    32401 gacttcccgg tgattgcaaa gctggagact taatccttat cgatgatggc aaagtaacag
    32461 ttcaggttat tgaggttaaa ggtagcgatg taatcactaa agttattgag ccaggatttg
    32521 tttctaataa caagggaatt aatcttccag gagttgcagt atcagttcct gccatgagtg
    32581 agaaagatat tgaagatctg cgctgggggc ttaaagcagg tgcggacttt atcgctttat
    32641 catttgtaag atctgcaaaa gatatcgatg atgtttacaa agtaatggat gaagtgggcc
    32701 atcgcattcc tgttatcgcc aagattgaaa aacctcaagc agtcgataat ctagaggaga
    32761 tagttctagc atttgatgga attatggtcg ctcgcggtga tctcggagtt gaaatgccga
    32821 ttgaagatat tccacttgtg cagaagcgat gcatcactct tgctagggaa aatgcaaagc
    32881 cagtaattgt tgctactcag atgcttgatt caatgattaa caactctcga ccaacgagag
    32941 ctgaggctac agattgcgct aatgccgttc ttgatggcgc tgatgctttg atgctttcag
    33001 gtgagactag cgtgggagca tttgcaattg aggcagtatc aacaatggct cgaatcattg
    33061 aaaagactga gaaggaagcg cttcatttaa ttccagctct gcaacacaac ccaaaaacta
    33121 aaggtggggc aataacaaag gctgcaaccg aggttggcct aacaattggc gcacaagttc
    33181 tggctgcatt cactaagagc ggtgattcag cgcgcagaat gtcaagactt agatcgccaa
    33241 ttccaatttt ggcgctcacc cctgatactg ctacctataa ccagatggca cttacttggg
    33301 gtgttgaacc acttctaacc ccattagtta ccacaactga tcagatggtt aagcaggtag
    33361 atacgatctt gattgaaagt aagagagtgg ctgttggtga gttaatcatg attgttgctg
    33421 gttcccctcc aggaattcct ggttccacca atgctatgcg cgttcataag gtgggagatg
    33481 ctgtcagtgg cgttgctcct gcctatcgct agaatattct tgaattaaat ctagcctcgg
    33541 tactccaacg gtagagaggg cggtctcaaa caccgcatag tgtcggttca aatccgactc
    33601 gaggcacgtg agtattaata ttaaatctgg ttcccatcgc atccttattg ctgaagatga
    33661 aactttaatc cgtatggatt tagttgaaat gctaagtgag tcgggatatg aggtagtagg
    33721 tcaggcaagt aatggccaag aagcaatcga tcttgctaaa gagctaaaac ctgatttagc
    33781 aatccttgat gtgaagatgc caatccttga tggaatctcc gctgcaactc agataatcga
    33841 aatctctcca gttctaatgc tgaccgcatt tagccagaaa gatttgatag agcgagcaag
    33901 tgaggctgga gcgatggcat atgttgttaa accttttaca atcaatgatc taatcccagc
    33961 aatagagatc tcaattagtc gtcatagaca gatgaaatct ctagcacttg aggtaagtga
    34021 tcttcatgaa agacttgaga gtagaaagtt aattgacaga gctaagggaa ttttgatgaa
    34081 ggccctaaac ctctcagagc ctgaggcatt ttcatggatt cagcgggcag cgatggaccg
    34141 gcgaatttcg atgaaagagg tggctaaagc ggtgattgac ccctcggccg ctcctaataa
    34201 gtaggtatgc accgcttttc atcaaaaagt aacaggaatg taattctcca agcgataaac
    34261 ccggttgcct cactgttgaa cctaaggcta accttcaccc ctccctgtcc cagggggcat
    34321 gatcatgaat gaaaggtact aaatgaaaaa gcgtggatta atttcggttg ctgcagcggc
    34381 cgcactggca gctgcgtcac tggttgcagt tgcaccagcg tcaaatgcag cagctaagac
    34441 aatcaccctc gcgtttcagg gtccactaac cggcgaagaa gctcaagttg gtcaggacga
    34501 attggctggc atgctttatg cagtcaaact ctttaacgcg aagaacaaag gtaagtatgt
    34561 agttaaggtt gttcaggcag atgaccaggg cgatccagca caatctgcaa aggttgctcc
    34621 aggaatcgca tcaaacaaga agatcattgg tcttgttgga gctgcttact caggtgcaac
    34681 tattgctgca cttccttact acaagcagac tctattgccg atgatttctc catcagcaac
    34741 acgtgtgtca attacaaacc cagccgctga aggtggcgca atgggtgttc ctgtattcca
    34801 ccgtgtacca ccaaccgaca aggttcaggg tccatcacta tttaagttag ctacaaaggg
    34861 tgtttcatca cctaaggtat tcgtagtcga tgatcaatca gcctatggcg ttggtctagt
    34921 tgattacatg aagcctgcaa ttcctgctgg tcaatcagct ggatttgata gcgtttctga
    34981 caagacaact gattggtctg caacgatctc taagatcaag actgcaggtg caaacgttgt
    35041 tatctacacc ggttatttcc cacaggctgc accattcttc aagcagctac gtgatagcgg
    35101 atacaccgga attcttgcag gcggcgacgg cgttctaagc ccaagcttcg taactcttgc
    35161 accagcatca gttctcgaag gtgttcgtct aactgcagga actgttcctc tttcagagat
    35221 cagtgccaag cttgaagctg atttcaagaa gagcatgggt aaggcctcag gcgtttacgc
    35281 tgctgagtcc atcgatgcta caaacgtatt cctaagctgt gttgcaaagg gtgtttcaac
    35341 acgcgatgca atgctcaagt gcgttaaggc atacagcggt aagtcactaa ctggtgcaac
    35401 cattaagttt gatgcttatg gtgatgtcgc tggtggcgta atgaacggct tcgaagttag
    35461 aggcggcgct atcaagttca caggaccagt aaagtaaaaa agtaggaaaa atagctttcg
    35521 gcaggctctc ttaattgagg gcctgccgaa actttaactc gaagttttag ataggaaatt
    35581 gggcgccttg ataggtaact ttctagatca gttttggtct ttgacttttg gtggactaag
    35641 ccttggcttc atttatattc tgattgccct tggctacacc atggtctacg gcgtcctgcg
    35701 catgattaat tttgcaaatt cagaaatttt tatggttggc acttttgcca caattattat
    35761 tattcgagat gtattcaaat ttacacaagc ttcagagcca gcaactggcg cacgcctctg
    35821 gttaattctt gcaacagcgc tgatattttc tatgttggtt tctggcgttc tatcaatgct
    35881 ggttgaaatt gttgcctacc gcagattgcg tagaatgaat tcaggtgttc tagctccatt
    35941 gatttcagca atcggtgttt ctatggtgct tgcagaaagc gttcgaattc ttacggattc
    36001 acgtcctgtt gcaagtcctc gagctatgga aaagaaattt attggtcagt tctttgatgc
    36061 cggagttaga atcgataata caataattgt cattgcagct cttatccttt ttgctgtact
    36121 tattaccttc gttaaccgaa cacgtatggg taaggcaatt cgtgcagtct caatgaaccc
    36181 taatgcggct cgtctgatgg gaatcaatat tgatggagta gtttcagcta ccttccttgt
    36241 tggcggattg atgaccggtg cagctggatt cttctacgtt ctaaagtatg aaaatactgc
    36301 ttcaaatata ggatttgagt tgggaattgc agcattcacc gcagccatct tgggaggaat
    36361 tggaaacatt aagggcgcct tctacggcgg tctcgcattg ggccttcttg aaacttatgc
    36421 atcattcttc cttggaacac aatggaaggc cgttacggtc tttattgtgc tcgtattgat
    36481 tctttacacc aggccgaacg ggttattcgg tgaagcgtta acccagacga gggcatgatg
    36541 ataaatcttc gcgataaggg tgctgaaatc tgggagagac aaggtcgcct tggcagagtt
    36601 ggtatagctc tcggaggtgt agcaattgct gcagctcttc catttatggg cgggaccatt
    36661 ctcaatactc ccattgctga ctatgaatca gttttagttt atccaattgc gatgtatgta
    36721 cttatggcaa tcggtctcaa cattgttatt ggaaagagtg gattactcga tcttggttat
    36781 gtcgcattct ttgcaattgg ggcctacacc atgggcttac ttggaacaaa gacagactta
    36841 aatacttggg aaattttgcc aattggaatt gcgatggcaa tgttttctgg aattattctt
    36901 ggagttcccg ctctgaaact tcgtggtgat taccttgcca ttattactct tggatttgga
    36961 gagattgtaa gaattgtagc gctcaacctt ggtgcacttg gacgatcaga aggaattatt
    37021 aatattccaa cccctccaac tattttcgga attgaatata gatttgatag ccatcgcatt
    37081 tattactgga cccttttggt tctaatattg tttgcaattt ggatggtgcg ccgactttcg
    37141 gttcgccgcg ccggtcgagc ttgggagtca attcgacagg atgaggatgc ggcagagtta
    37201 cttggtgtcc ctacatttaa atacaaagtt tgggcattcg tgcttggggc ttcggttggt
    37261 ggagcagccg gtgttttcta tgcctcaaag acactattta tagcaccaga taatttcact
    37321 cttcaaatct caattttgat actagcctgt gtggtgtttg gtggaatggg aaatatctgg
    37381 ggtgtaattc ttggcgctgt aattcttggt tatctgccag atcgaattcg cttcatctct
    37441 gacgcgagac ttctagtctt tggtctagtt ttggtaatca tgatgaatct tcgccctgat
    37501 ggcttgttgc cacgcaagaa acgtgaaaag gccatcgtca gaaaggatgc atcatgactt
    37561 catccttgat acagctcaaa aatgtcacaa tgaaatttgg gggagttact gccctaagtg
    37621 atgtgacatt tgatgttaag aagcaagaaa tccttggtct aattggacct aatggcgctg
    37681 gcaaaacaac ggtctttaat gtaataacag gtgtttatca gattacttct ggtgagatta
    37741 tttttgatgg aaaatcttta tctggagtga agcgttataa aataacaaag agaggggtgg
    37801 ctagaacttt ccagaacatt cgactctggg gagatatgac agccctcgaa aacgtaatga
    37861 ctgcaactga tacccacaaa aagtctggcc tggtatctgg attattcggg ttcccaattt
    37921 ctagacgtga agaaaaacgc gacaaagcaa gagcgcagga gttattggat tttataggaa
    37981 ttggcgagta tgcagatcgc ttagcaaaga atcttccata tggcgtacag agaagacttg
    38041 agattgcaag agcgatggga gcggagccaa agctacttct acttgatgaa ccagcagctg
    38101 gatttaatcc cgcagaaaag gttgagctag cagcaacgat tagaaagatt cgtgatgctg
    38161 gatataccgt gctcttgatt gagcatgata tgtcgctagt aatgaaagtc tcagatcgcg
    38221 ttgttgttct agattttgga cagaaaattg ccgatgactc gcctcgaaaa gttcaagatg
    38281 atccgagagt tattgaggct tatttaggag tacctgaaga tgcgtcttga aattaaagat
    38341 cttcgtgttg cctatggaaa aatcgaggca gttaagggca tttcaatcgt agtgaaccag
    38401 ggtgaaattg tctctctcat tggagccaat ggtgctggaa aaacaactct tcttaagact
    38461 atttcagggc tgttaaaccc aacttctgga tctattacat acgatggtga agacattctg
    38521 aaatacaaag ctcaccaaag agtttcactt ggcatttctc aagctccaga gggtcgtgga
    38581 atatttcctg gaatgacggt tcttgaaaat cttgaacttg gtaaatacca cctcaagatg
    38641 aagaagtctg agattgcaga ggatctagcg acagtttatg agttatttcc acgtctaaaa
    38701 gagcgtgcca ctcaagcagg aggaactctc tcgggcggcg agcaacagat gcttgcgatt
    38761 gggagagcgc ttatggcaaa gccaaaggtt cttcttcttg atgagccctc aatgggactt
    38821 gccccgcaga tgattgccaa catttttaga attattaccc aaatcaataa aatcggagta
    38881 acaatacttc tagttgagca aaatgctcag caagcgctac aacgttcaga tcgagcctat
    38941 gtattagaag ttggaaatat cactaaagaa gccaagggca aagacttact aaatgatccg
    39001 catgttaagt cagcttatct tggaactgga gcgcacgaac tagattagtt agcgctgagc
    39061 gagaattaag caagtaattc ttgcggtgca ggttttctcg cctttatcgt ttgtaatctc
    39121 tacgttgtaa gtgcagagag tttttccaag tgttactgct gttgcaactg ctgttacata
    39181 tccttctgtt gcgctcttgt gatggcttgc acttatctca attccaacaa ttcttctaga
    39241 gggtcctgca tgtagatggg ttccaactga gcccaagctt tctgctagaa caacatttgc
    39301 tccaccatga agtaggccaa aaggttgagt atttccttca actggcattc ggccaactag
    39361 gcgctgcggg ctagcttcaa tgatttcaat tcccatcttc ttatcaaggg ccccgcggcc
    39421 acgatcattt aatatctcac tgaagtctgt catgtgggca attgtatgtg gcaatgccta
    39481 agattaagtg tgatgaagaa gaagttgctc atactagatg gccattcaat ggcctttagg
    39541 gctttctatg ccctgccagt tgagaacttt tcaacctcga gcggtcagcc cacaaatgca
    39601 atctatggtt ttgcctccat gctgattaac ttaatcagag atgagaaacc aactcatatt
    39661 gcaactgcct ttgatgtatc tcgcaagacc tttagatctg aaaagtttcc ggaatataaa
    39721 gcaaatagag ccgccactcc tgaggagttt cgctctcagg tctcctacat taatgagctc
    39781 ttggatgcct ttgcgattcc acattttgaa cttgagggct atgaagccga tgatgtaatc
    39841 gcaacttttg tgaagcactt tagcgacgag gcagagatat acatctgctc tggagatcgt
    39901 gattcatttc aattagtaag tgatcaggta accgtcctct atccaaaaaa aggcgtgact
    39961 gaactttcca gaatgacgcc cagtgcagtt ttcgaaaaat atgggctaac gccaaggcaa
    40021 tatcctgact ttgctgccct tcgaggtgat ccaagtgata acttgccatc aatacctgga
    40081 gttggcgaga agacagcaac taaatggatt caagaatttg gctccctttc agagttgatt
    40141 gccaaggtag ataccgttcc aggcaaagca ggggaggcct taaggacagc gctgcctgca
    40201 gtaataacca atcgtgaatt gactcaactt agaaacgatt taccgctatc gcctgactta
    40261 acagatcttg catggagcgg tgttgagata ttaaaagtga atcaattatt tgaaaaacta
    40321 gaaattaaag ctctcaaagc aagggttgct ccctttgcta aaggagggca gggaaaagaa
    40381 gttgttgcaa aaaaggcgag cgttcgcaat gtaagtaaaa gtgaatttga aaaagcactt
    40441 agctctagca ctggccttgt gggcctaata ctttcagaga accaagcagc aatttcgatt
    40501 gagcccgagg tggtcttggt tgcaccgctt agtgaggtat cagattcgat aactagcttt
    40561 gatggtttta tctttcatgg cgcaaaaaaa gtaatctcct ctaagctttt gagatcggta
    40621 gctgttgaca ctgaagttgc tgcctattta ctcaatgctg gttcaagaga tattgcacta
    40681 gctgaccttc tgcagaagta tcttggtgtt gaaagtgaaa acacacctac agatcttttc
    40741 tccacagatt ggaatccaag tatcgcctcc cacctaggtg aactctgggc aacgctttca
    40801 aaagaactag aggcaatgag ctgccttgat ttatataacc agatagagat gcccacgatg
    40861 catgtgttgg ccgaaatgga ggcaactggc atcgcaatag ataaagcgga gatgaaatct
    40921 ttattgagct tctttgagaa agaagaggca gaggcaacat cccttgcata caagtcggtt
    40981 ggacacgaat ttaacgctgc ctccccgaag caattgcagg ttgttttatt tgaagagcta
    41041 aagcttccta aaacaaagcg gattaaaact ggctttagta ccgacgctga atctttagag
    41101 tggctttatt caacaactaa gcatccagtt ttagcttcgc ttctgcgaat tcgcgaagtt
    41161 ggaaagctta gaacaactgt tgatggatta cttaattcaa ctaaaggtga tagcagaatt
    41221 cacaccacgt ttcagcaaac cacgacggca acaggaagac tttcatcaac agagccaaat
    41281 ttgcaaaata ttccgatcag aagcgatgag ggcagaagaa ttagaaactg ctttatcgcc
    41341 caagatccat tcgttgatct attaactgct gattacagcc agattgagat gcgcattatg
    41401 gcccacttat ctgatgacaa aggactactt gaagcattta gaaccgggga ggacttgcat
    41461 tcaacagttg gcgcacaagt atttgatgtt ccagtatcta aagttgatgc agatatgaga
    41521 aggcagatta aggctatgtc ctatggtctt gcctacggtc tttcctctta tggacttgcc
    41581 cagcaattag atatttcacc aaccgatgca agtattttga tgagcaagta ttttgaaaga
    41641 tttggaggca ttcaagatta tttgaaagaa gttgtaaaaa ttgctagaga aaaaggttat
    41701 accgaaacga ttctgggcag aaggcgctat ctacctgatc taaaccatga aaatcgtggc
    41761 agacgtgaaa tggcagagcg gatggctctt aatgctccaa tccaaggatc tgctgcagac
    41821 ataattaaag ttgcaatgct aaatgttgaa aaagccatga aaagtaagaa tttatcatca
    41881 aggctattac ttcaggttca tgatgaatta atctttgaaa tcgccaaggg tgagcatgag
    41941 gtcatggaag aattggtaag aaagcagatg ggaagtgcct ttgagctaaa ggctccgctt
    42001 gatgtcaata tcggctttgg aaagagttgg gatttagcag cccattagcc tcaaattgac
    42061 cgataagcgc attagaaagt agatttacgc cctcgcttaa ggttcctgtg cctcctcgga
    42121 cgtagtagga agcgcccctt tgctagtaga aaatctacaa gtagatggag tcggactttg
    42181 tgcgtgccct actagaaaaa ttccccacgg agtaccttgt caactcaaat aacagtaaac
    42241 gatgttggaa atgcagaaga ttttctcgcc gcaatcgaag gcacaataaa gaattttaat
    42301 gatggagatc tagtatctgg cgtaatagtc caggtagatc gcgacgaagt cctacttgat
    42361 attggttata aaactgaagg cgtaattcca gctagagagc tctcgatccg ccatgacctt
    42421 gatccaagtg aattggtaaa agttggagat cgcgttgaag ccctagttct gcagaaggaa
    42481 gacaaagaag gtcgcttaat actttctaag aagcgcgcac agtatgaaaa agcctggagc
    42541 gatattgaag gcaagaaaga gcgcgacgaa gttgtcacag gtacagtaat tgaagttgtt
    42601 aaaggtggat taattgttga tattggccta agaggctttc ttccagcatc ccttgttgag
    42661 atgcgccggg ttagagatct ctcgccatat attggaaaag aaatagaagc ccgcattatt
    42721 gaacttgata agaaccgtaa caacgtagtg ctatcccgta gagcattttt agagcagagc
    42781 caatctgaat cccgcacaac tttccttaat cagttgatca agggtcaagt gcgcgaaggt
    42841 gtagtttcat caatcgttaa ctttggtgca ttcgtagacc ttggcggcgt tgatggtctt
    42901 gtccacgtct ctgagctctc ttggaaacat atcgatcatc caggtgaagt tgttgcagtt
    42961 ggcgatgagg ttactgttga agttctagaa gttgattttg agcgtgagag agtttcactt
    43021 tccctcaaag caacacaaga agatccatgg caggcatttg ctcgcaccca cactattaat
    43081 caggtagtgc caggtgaggt tactaaatta gttccattcg gcgcctttat aaaggtattc
    43141 gatggaatcg aaggtttagt tcacatctca gagctagcag aacgccatgt tgaaattcca
    43201 gagcaggtag ttcaagttgg agactctttg ttcgtaaaga tcatcgatat tgatttagag
    43261 cgccgccgca tctcactttc tcttaagcaa gcaaatgagg gacaagatgt tgcagttgaa
    43321 gcttttgatc caactcaata tggaatgcca gctcgctatg atgaaaatgg aaactttatt
    43381 tatcctgaag gctttgatgc ttcaacccag gagtggcagc caggatatga aacacagcgc
    43441 caggagtggg agcggcaata tgctgaagct caaactagat tcttggctca taagaagcag
    43501 aaggcggagg ctcaagcagc cgatgctgca gctgcctcag ttgctacccc agaagccgag
    43561 taactctgcc ccgttttaaa tcactgggtc cttctttgaa gggcccagtt ttatttttct
    43621 aggtaggctg aagcagtgtt aagagttgcg ttaactggcg gtatcggatc aggtaagagc
    43681 ctggttggtg agattctgga agaacttggc gcattagtta ttgattctga tcaattagcc
    43741 cgtgaagtca ttgaacgagg aagcccagga tatgaagaag ttgttaccgc ctttggcgac
    43801 tcaattttaa gtgaagggca gattgatcgt tcaaaattgg cagccgttgt ctttaaagag
    43861 agcgatttac gaaagaaatt agaatcaatt attcaccctc tagttcgaga agctgctgag
    43921 aagctcgcaa gaaagctgcc gagcggagcc attttaatta atcagattcc cttacttgtt
    43981 gaaagtgatg gcgctaagag atttgactat gttgtaacag tctcagcaga tgaggaaata
    44041 agacgcgagc gtttacgttt gcggggttta aaggattacg agatcaccca gagaatggca
    44101 gctcaagtca gtgattcgga gcgggagaag atcgctaatt acatcattag aaatgatgca
    44161 agtattgatg agttaactcg ggcggcggag gcgttaatgg ccaatgagct cctgcctaga
    44221 gctcagaagc aaggtttgta atatggcgcg ggcagttaca tcaacaccaa gagctaatcg
    44281 ggcctttgag gtcatcagcg attttaagcc agctggagat caaccagatg ctattgcaga
    44341 gctgactcgc agaataaata gtggtgagag cgatgtggtc ttactcggag ctactggaac
    44401 tggaaagtct gcaaccactg cttggctaat tgaggcagtt cagcgtccaa ccctagttct
    44461 tgcgccaaat aaaactcttg cagctcaact tgcaaatgag tttaaagagc taatgccgaa
    44521 taatgccatt gaatactttg tctcttatta cgactattac caacctgagg cctatgtccc
    44581 gcagactgat accttcatag agaaagactc ctcagttaat tctgaagttg agcgtcttcg
    44641 ttactcggcc acatactcac ttttaacaag gcgcgatgta atagttgttg cttcggtctc
    44701 atgcatatac ggccttggta ccccgcagga atatatcgat cgaatgatca ctctagctgt
    44761 gggagataaa attgatagaa atgttttact caggcgtttt gttgatatcc agtacaagcg
    44821 aaacgatatg gcctttgaaa ggggaacttt cagagttcgt ggtgatactg ttgaaattat
    44881 tccagtctat gaagagctcg ctaccagaat tgaattcttt ggcgatgaaa ttgaacgctt
    44941 aacaactcta catccactaa ctggtgaaat tgttagtgat gttaaagaag tattcatatt
    45001 tccagctact cattactcag caggacctga gacaatgaag cgagctattg ccaatatcga
    45061 agcagaacta gatgttcgcc ttaaagagtt agagaaggcc aataagctac ttgaggccca
    45121 gcgccttcga atgcgaacca catttgatat tgaaatgatg ctgcagcttg gcttttgttc
    45181 tggaattgag aattactcaa ggcatataga ttttcgagag ccaggctctg ctcctaactg
    45241 tctactagat tattttccag aggacttcct ttgcgtaatc gatgagtctc atatcactgt
    45301 tccacaaatt ggggcgatgt atgagggcga tgcatcaaga aagagaacgc ttgtggagca
    45361 tggttttcgc ctgccaagcg ctcttgataa ccgtccttta aagtttgccg aatttctaga
    45421 gcgaacccca caaaaggttt atctctcagc aacgcctggc ccctatgaga tggagaaaac
    45481 agggggagta tttgttgagc aggttatcag gccaactgga cttgttgatc ccaagattgt
    45541 tctaaaacca ataaagaatc aaatcgatga cttaatgtca gaaattaagg taagagctga
    45601 gaagaatgag cgggtcttgg ttacaactct gacaaagaag atggcagagg atttaactga
    45661 tttcttcaca gatgctggaa tcagagttcg atatttgcac tcagaggttg atactttaag
    45721 aagagtggaa ctgcttagag aattaaggct tggcgaatac gatgttctta tcggaatcaa
    45781 cttgctacgt gagggtctag atcttcctga agtatcactt gttgcaattc tcgatgctga
    45841 taaagaggga ttcctgcgct caactcgctc gctaattcaa actattgggc gcgctgcccg
    45901 aaatgtttca ggcgaggttc atatgtatgc cgacaagatc actgattcca tgactagagc
    45961 aattgatgaa accaatcgaa gaagagctaa gcaggtggcc tataaccttg cagcagggct
    46021 tgatcctcag ccgcttcgta aaaaaattgc agatatcacc gatttaatcg ccgaagagag
    46081 cgatgacacc aacgctttgc tggctggaaa taagaagcgc gggatgagtc agactccagt
    46141 tggcgtgcat gccaaatcac tcgtagcctt accgcttgcc gagttactgg gccttataga
    46201 ttctctaacc gagcaaatga gaaatgctgc agctgaactg caatttgaac ttgcagctcg
    46261 tctgcgagac gagataaaag agttaaagag ggagcttcgg ggaatgcagg aaggaggcat
    46321 gtgatgagcg cagatcgttt aatagtccgt ggtgcaagag aacataatct aaagaatgtc
    46381 tcccttgatc ttccaagaaa tgccatgatt gtttttaccg ggctatctgg atctggtaaa
    46441 tcatctcttg cctttgacac aatatttgct gaaggtcaga ggcgctatgt cgagtccctc
    46501 tctgcctatg cgcgccaatt tctaggacag atggataagc ccgatgttga cttcattgag
    46561 gggctatctc cagctgtatc tattgatcaa aaatcaacta atcgaaatcc aagatcgaca
    46621 gttggaacta ttactgaggt ctatgactat cttcgtcttc tcttcgccag agcaggaaag
    46681 ccacattgcc caaagtgcgg aaaagagatt gcaaaacaga ctccgcaaaa tattgtcgat
    46741 caaatcctcg agcttccagc agagactaag tttcaagtat tagcacctgt tatcagggcg
    46801 cgaaagggag agtttgttga tctcttcaaa gatttcattg ctcagggata ctcaagagtc
    46861 agagttgatg gaactacagt tgccttaact gaggctccaa aactaaagaa acaagagaaa
    46921 cacacgattg aagtagtaat tgatcgtttg actgcaaagc cagagggaag gcaacgtcta
    46981 actgattcaa ttgagacagc gctaaagcta gccaatggcc ttgtaactct agattttgtt
    47041 gatgcaaaag gcgcagataa agagcgaact tatagcgagc tcctagcttg ccatgattgt
    47101 ggactttcat tccaggagat ggagccaaga tcgttttcat ttaattcacc atttggagcc
    47161 tgcgctgagt gcactggcat aggttcaaaa cttgaagttg atgaagggct agtgattcca
    47221 gatgattcaa tttcaataaa tgatggcgca attgctcctt ggtccggtgg gcagtcttcc
    47281 gactacttta taaatctctt ggaggctcta gctgccgatg ttaagttctc cctaaatacc
    47341 ccctggagca aactctctgt taaagctaaa gatgcaattt taaatggcta tgaatatgaa
    47401 gttcatgtta agtacaagaa tcgctatggc cgagtaagaa attactcaac aggatttgaa
    47461 ggagtcattc cttttgttca tcgcaggcac tcagaaactg atagtgatta cagcagagag
    47521 agatttgaag cctatatgcg ccaagtccca tgtcctgcct gtaagggttc tcgtctaaag
    47581 ccagaagtat tggccgttac cttaggaagc aaaaacatcg cagagatatg cgagctttca
    47641 attgaggatt gcgcgaagtt tttaaagggc ttaaaacttg catcacgtga ggcgaaaatc
    47701 gcagagcgcg taatgaagga agtacatgct cgacttgggt ttttacttga tgttggactc
    47761 gaatatttga ctcttgcaag acctgcagcg acgctctccg gaggtgaggc gcagagaatt
    47821 cgccttgcaa ctcagattgg ctcaggctta gttggagttc tctatgttct agatgaacca
    47881 agtatcggtc ttcaccaaag agataataag cgcctgatag agactctgac gcatcttaga
    47941 gacctaggaa ataccttaat tgtcgttgaa cacgatgaag agacgattag aactgcagac
    48001 tggattgttg atattggtcc aggagcggga gagcatggcg gagagattgt cgtatcaggg
    48061 gattatcaag ccctgattga ttcaaagaag tcaatcactg gagcctacat atcaggtcga
    48121 tcaaaaatag atatccctaa gaagcgacga gcaattaacg ctaagcgagt tttgaccatc
    48181 aaggaagcga aagagaataa cttaagaaat gttgatgtaa ctatcccgct aggagttttc
    48241 gttgccgtta caggagtcag cggctctgga aaatccaccc tggtaaacga cattctctat
    48301 tccgtacttg caaataagtt aaatggagct cgaatagttc ctggtcgcca tagaagtatt
    48361 actggtatcg atcaactaga taaagttgtt cacgtagatc aatcaccgat tggtagaact
    48421 ccgcgctcaa accctgctac ctacactgga gtctttgaca agattcgtgc gctattttct
    48481 gaaacaagtg aagcaaaagt gcgtggctat caacaggggc gcttctcatt taatgttaaa
    48541 ggtggaagat gtgagaattg ctctggtgat ggaactatca caatcgagat gaacttttta
    48601 cctgatgttt atgtgccatg cgaagtctgc catggagcaa gatataacag agagacttta
    48661 gaggttcatt acaagggcaa gacaattgct gaagttcttg atatgcccat tgagaaggct
    48721 caagatttct ttgaatcaat tcctgccatt caccgctatc taaagacgct ctgtgatgtg
    48781 ggccttggct atgttcgtct tggtcaatcc gcaccaactc tttcgggagg agaggcgcag
    48841 agagtaaagc tttcaacaga gctacaacgc cgatcaacgg ggcgaacaat atatgtactt
    48901 gatgagccaa cgacaggact tcactttgaa gatgtaagga aacttcttgg cgtgcttcaa
    48961 agactagttg atactggaaa tactgttgtt gttattgaac ataatatgga tgttattaag
    49021 tgcgcagatt ggattattga cctaggaccg gagggcggat ctgggggagg aagcatcgtt
    49081 gctgaaggag ttccggaggc aattgcaaag gtttctgcca gctataccgg aaagttcctc
    49141 cgcgaaacac taaagaagta gccatggcag atcctgcttc ataccgccca gagaatgttc
    49201 ccgatgaacc tggggtctat cgcttcttta acgaaaaaga tgaagttatc tatgtaggaa
    49261 aggcgagatc gctaaagaat cggattaact cctactttca aaaaaacgtt ggcgagaaaa
    49321 cttatcgaat gattcattcc gctaatagag ttgattggac gctagtaaga agcgaagttg
    49381 aggcgcttca attggaattt acctggatta aagaggagaa ccctgccttt aatgtgcaat
    49441 ttaaagatga taagagctat ccatatctgg ctcttacaat ggatgagaga tatccacgtc
    49501 tactcataac taggagagca aaacagaaag gtgtcaccta ctttggcccc ttcacccatg
    49561 cctgggcgct tcgctcaaca tttgatgttc tcctaaagct ctttccagtt agatcatgtt
    49621 caaatagtaa ttttgaaaga gctcagcgag ctaagcgtcc ctgcctacta ggagatatcg
    49681 ggaaatgcgc cgctccatgt gttgatcgaa ttaacgaaag cgatcaccga attcttgccc
    49741 agcgcctagg cgaattcatc tctgatggtt cagcagatat tactcaagct ctgcgagatc
    49801 agatggctga ggctgcaaga gcagaggagt ttgaaagagc cggaaaatta cgagatcaac
    49861 tagaggcgct agagcgggca agtgaatcaa ctgatagcgc tctatccgac tccatattct
    49921 ctgacttcat tgcaattcac caagacatca cccacgctgc aatctcgata tttagagtca
    49981 gggctggacg cgtcatcagc tcgcgctctt ggatgattga tttagcaaac ctgccggagg
    50041 attcctcact acttgatgct tgtattaatc gggtttatca agattttgaa attggcaagg
    50101 aaatattagt taatcaggaa atcgatggag acgcgcttgc agaatatcta tcagagagag
    50161 ctggatataa ggtaactatt aagaagccag agcgtggcga gaagagtgag atcttggata
    50221 tggttaagcg aaatgctaac caagccttaa ttcagttttt aagtaaaaga gcaaatgatg
    50281 ctggagttag cgggcgagcg ctagaggact tagcaagtgc acttaatttg gaagagctgc
    50341 cattaagaat cgaatgcttt gacatttcaa atatccaagg aacctcagtt gttgcatcaa
    50401 tggtcgtatt tgaagatgga caggcgaaga agagcgagta tcgccgcttt ggaatcgatg
    50461 ataaagaaaa gtttgatgat acaagggcga tgcatcatgt tattactaga cgatttaaga
    50521 gatatctagc agagaaggat ttagatctat cagaaataga gttaagtgga gggccgcgac
    50581 ctaagtttgc ctatccgccc cagctagtaa ttgttgatgg tggcaaggga caggttaacg
    50641 ctgctgccag agcttttgct gaacttggaa ttacagatat cgccttggtc ggacttgcta
    50701 aaagacttga agagatttgg tttgctgatc agagctatcc agtaattctt gatcgacata
    50761 gcgaggctct ttatttggtg cagaggattc gcgatgaggc ccatagattt gcagtgactt
    50821 tccaccgctc aaagcgctct aaattaatgc tggaatcact tcttgatgaa atcgataatc
    50881 tcggagaggt tagacgagca gttttattgg agcacttcgg atctattgca gcccttagta
    50941 aagcaagcct tgaggagata gcagctcttc caggaattgg acctaagagc gctgaaagca
    51001 ttctcgcagg ccttgctaat tcagcttctg gctacttcgt tgatacctca acaggtgaaa
    51061 taattgagcg ttcttgacac acctaggatg atgtagacat gggacgagaa atagtagtta
    51121 taaccggtat gagcggagca ggtcgctcaa cggtggctca tgctatggaa gatcatggct
    51181 ggtatgtaat tgataacttg cccccttctt tattaattcc actctttgat ttgactcaga
    51241 gcagcgatga ttcaattgct gtagtaattg atgtgcgggg aagacaattc tttgatgatt
    51301 tgaataaaac cctagctgag ttggcagaga ggggaatatc tcgaaaaatt atctttgttg
    51361 acgcctcaga tgatgtttta gttcgaaggt ttgaatcgtc tagacgtccc caccctcttc
    51421 agggaagtga tcgaatcctc gatggtattg agaaggagcg ggagcgacta agagaacttc
    51481 gctctggggc agatcttgta gtggattcat cgcaattaaa tgtccatcaa ttagagagaa
    51541 aaattgccga gctattctcg gtagatcctt cagcctcgct aaaagttaat gttctctcct
    51601 ttggatataa gttcggtctt ccagttgatg cagacttagt tatggattgc cgctttattc
    51661 caaacccaca ctgggatccc gaacttaggg cgatgaatgg tcttgataag caagttagtg
    51721 aggcagttct tagcgctgag agcgttagtg aatttttaga gcgatactta gctctcttta
    51781 atagcttagg acaaggatat ttgagagagg gaaagaaata tataactctt gcaattggtt
    51841 gcactggcgg aaaacataga agcgtcgcgg ttgctgaaga gcttgcaagg agaattaatg
    51901 gcagcgcaat tgatgcagaa catagcgttt cggctcacgc gattcataga gatttaggta
    51961 gggagcgttg agtaacccca aggttgttgc acttggcggt ggccatggcc ttgctgccac
    52021 attatctgct ttaagaaaaa tcactaatga gttaactgca attgtcactg ttgcggataa
    52081 tggtggatca agtgggagat taaggaaaga atttaattca cttcctccag gagatttaag
    52141 aatggcactt gctgcgctct gcgcagatga tgaatggggc agaagctggg cagagataat
    52201 gcaatatcgt ttcacctcaa aaggtgagtt aaatggccat gcacttggca atttactatt
    52261 aaccgcgctc tgggatagcg aaagcgatcc ggtcaaaggg ttagataaag ttggtgcgct
    52321 attaaaagtt gtagggcgag tactgccaat ggcactagaa ccccttgata ttgaaggaac
    52381 ttttaaaact tggcaaggca ctcacttaat tagaggacaa caagaggtag cgctaggacg
    52441 aggaatcctc gaagatttaa ggctaattcc ggatgatcca acgccaactt ctcaaggagt
    52501 tgatgcaatt gcaaaagcgg actttattac cttaggtcca ggctcctggt tttcaagtgt
    52561 tctgccacat cttctagtaa ccaaacagcg tcaagtgctt gagagttcaa gggctaagaa
    52621 agtgataatt cttaatcttg atgcctcacc gagcgccagt ggggatgaat tggctggtag
    52681 ttctccagca gatcatttga gaatcttgcg cagatttgcc ccagatctaa actgtgatgt
    52741 tgctctacta gatagctcaa tcgccgctcg ttctgagcta ttaatagagc tagagggcct
    52801 tgttgaggcg atgggtggaa gggtatttgt tgcagatctt gcatctcacc ccggaagtaa
    52861 tcaccatgat actgaaaaat tatttttgac tttgagccac atttttcaag ccgagatgct
    52921 tagataaccc acatggcaat gactgcggcg gttaaggacg agttaagccg ccttactatc
    52981 tcaaagcctt gctgccgaaa agcggaagtt tctgcgatat tgcgctttgc cggggggcta
    53041 catatctcag ccggaaagat tatggttgag attgaacttg ataccgccca agctgccaga
    53101 agaataagaa aagatattgc tgatatctat ggccatgatt cagatttagc agttattagc
    53161 gctggtggtt taagaaaagg tagtcgctat ttagttcgag taattaaaga tggcgataac
    53221 ttagcaagac aaactggact tgttgattct catggacgtc cagtaagagg tttaccaccg
    53281 caagttgtct ctgcaggaat ttgttgcgcc gaagctgcat ggcgaggtgc atttcttgca
    53341 catggttcgt taactgaacc tggaagatca tcggctctag aaattacctg cccaggacca
    53401 gaagccgcgc ttgctcttgt tggcgcagct cgcagattaa cgattgctgc aaaggctcgt
    53461 gaggttcgcg gagttgatcg agtagtaatc agagatggcg atgcaattgg agttttatta
    53521 actcgtcttg gagcccatga aagtgttatg gcttgggaag agcgaaggat gagaagagaa
    53581 gtcagagcaa ctgccaatcg ccttgcaaac tttgatgatg ccaacttaag acgctcagca
    53641 agagctgcag ttgctgcatc tgcgcgagtt gcaagagcga tggagatttt aggagaagat
    53701 attccagatc atcttcgtga agcaggacag ctacgaatta cacatgggca ggcaagtctt
    53761 gaagagttag gttcgctatc aacgccgccg atgactaagg atgcaattgc gggacgaatt
    53821 aggcgcctat tggcgatggc cgataaacgc gcgagcgatc taggaattcc agatactgag
    53881 gccgggctct caccagagct actcaattaa tctttgagcc caagttatca aggcaagcgt
    53941 tctccaaata ttcactgata ggatttgccc aagtccacaa agtcgtggca agcctggtca
    54001 aaaattaaaa tagtggcgca ttagtcgcct attccgatgg agcgtggtta tcttggtaaa
    54061 agttggtatc aatgggtttg gtcgtattgg gcgtaacttc tttcgcgcag cgctaacatc
    54121 aaaggctgat attgaaatcg ttgccgtaaa tgacttaact gacattgcca cccttgctca
    54181 tctacttaaa tatgactcaa ttcttggtcg acttggccaa gaggtttctc acaatgaaaa
    54241 ttcaataact gttggaaata agagctttaa agtatttgca gagcgtgatc cagcagcgct
    54301 tgcctgggga tcagttgggg cagatatcgt cgttgaatca actggacatt tcacaaaagc
    54361 tgccgatgca aagaaacacc tcgttggggg agcaaagaag gtagttatct cagctccagc
    54421 aagtgatgag gatgtcactc tagttcttgg agttaatgat tctgcctatg atccagcaaa
    54481 gcatcagatt atctctaacg cctcttgcac aactaactgc cttgctccaa tggctaaagt
    54541 gctacacgat aactttggaa ttattcgagg ctttatgacc acagttcatg cttataccaa
    54601 tgatcaagtg atcctagatt ttcctcataa agatctacgc cgggcaagag ctgcggcaac
    54661 aaatatcatt ccaacttcaa ctggagcagc aaaggcaatt ggtttagttc ttccagagtt
    54721 gaagggcaag cttgatggct acgctctacg tgtaccagtg ccaacaggat caattaccga
    54781 tctaacagtt gagctatctc gcgaggtaag cgctgctgaa attaacgagg caatgaagaa
    54841 ggcggcagca ggtcccctta agggaattct ttattacacc gaagatccaa tcgtctctag
    54901 cgatattgtc accgatcctc actcctgcat ctttgatgcc ggaatcacta aggcaattgg
    54961 caatcaagca aaggttgccg gttggtatga caatgagtgg ggctattcaa atcgcctagt
    55021 tgatttaatg agctttattg ggaagagcct ttgattcaag gtttatcctc tttaaatcca
    55081 gcagggaaaa gagttcttct acgctgtgat ctaaatgttc ctctcaaaga taacggtgat
    55141 ggaaagagag ttattaccga tgatggtcgt atccgcgcat cgcttccaac gattcaagaa
    55201 ctactctcac taggtgcatc ggttgtaatc attgctcacc ttggaagacc aaaaggtgaa
    55261 gttaaaagag agttatcact agagccagtt gctaaacgct tagcagaact acttggaagg
    55321 caagtcgcat tttcaagtca gatagtaggg ccagaagctt tcgaaaaggc tgctgcctta
    55381 aagagtggag aagttcttct actagaaaat attcgcttta acgcatctga gactagtaaa
    55441 gatgagacag agcgtttagc tctagcaaaa gaattagcaa agttgggcga tctctatgta
    55501 ggagatggct ttggcgctgt acatagaaaa catgcctcag tatttgagct agcccaatta
    55561 ctcccgcacg ccgctggaaa attaatcgca gcagaggtcg ctgttcttga gaaactaact
    55621 caatctccag agcgtcccta tggagtagtt cttggcggag ctaaggtctc agataaaatc
    55681 ggagtgattt caaatctact tgataaagta aatattcttg caattggcgg aggaatgctc
    55741 tttacctttc ttgcagcgca aggaaaagaa attggaaaat ctttatttga agcggaatct
    55801 gttgacttgg ttaagcaatt attagaacga gcagaaaagt tgggagtaag agttttacta
    55861 ccaagtgata tctgggtagc tccagctttt gctaacgaat ctccaagttt agtaagcgca
    55921 gatcagattc caagtaatca gatggggcta gatataggcc cggaaagcgc ccatgcattt
    55981 gcttctgcaa ttaaagagtg cgccacagta ttttggaatg ggcccatggg agtttttgaa
    56041 tttcctaatt ttgcctcagg gaccagaatt gtcgctcaag ctttaagtga agtctcaggg
    56101 ctttccgttg ttggtggcgg agattcagca gctgcggtga gagctcttgg ctttagcgat
    56161 agcgattttg gatatatctc aactggcggg ggagcatcac ttgaatactt ggaaggtaag
    56221 cagcttccag gactaatagc cttaagataa gcaagtagcc aactaaggag cagaaatgag
    56281 cacacgtaaa ccgctgattg ctggcaattg gaagatgaat ctaaatcatc ttgaagcaat
    56341 tgctgttact cagaagttgg cctactcact agaggatcgt gactttgatg cagttgaagt
    56401 agcagtactg ccgccattta cagatttaag atcagttcaa actttagttg atggagatcg
    56461 tctcaagcta tcttacggag cccaagatat ttcaccggaa aagtctggtg cattcactgg
    56521 cgacatctct ggtgcgatgc tcaagaaact tgattgcacc tatgtgctag cgggacactc
    56581 tgagcgacga gcaatacatg gcgagagtaa tgagttaata aatgcaaaga tgcgcgcaat
    56641 attagataac gagatggtgc caattttttg tattggcgaa gaattagcaa tgcgcgaatc
    56701 aggagcacat gtaacttatg taattaacca gcttcgccaa gggctaaagg gatttcataa
    56761 accagattta aagaagatcg tttttgctta tgaaccagta tgggctattg gaactggaaa
    56821 gactgctact ccagatgatg ctcaagaggt ctgcgcagct attcgcctag aacttgctga
    56881 tattggaagt gatgagattg ctcaaaatgc cagaattctc tacggcggct cagtgaagtc
    56941 ggcaaatatc gtagaaatta tgcgacagag cgatatcgat ggcgccttgg ttggcggggc
    57001 cagccttgat gctgaggagt tcgcccgctt atgcaaattc caccgggttc tctagcgccc
    57061 agattcgtta ggaatcaccg cctttaggta gtatttgccc cttattcaac ccaaggagaa
    57121 ataagtgtcg ttagccctct caatccttct ggtgattacc agcatcttga tgattctctt
    57181 agttctgctc cataaaggaa aaggcagcgg actttcagat ctatttggcg ggggaatgtc
    57241 ctcaacctat ggtggatcat ccgttgttga gaagaactta gatcgcatca caattgtggt
    57301 cggcggtctc tggtttgcca tcatcatcgc acttggttta gttttaaaat aaaagattta
    57361 ttaatctcta gaaaagggag caattaaatg gctggtggaa gtgctatcag aggttcacgc
    57421 gttggcgcag gcccaatggg cgaggctgaa cgcggcgaag ctattgcgcg ctttcacgtt
    57481 tcttactggt gccaaaatgg ccatgaaaca aaaccttcct ttgctgaaga tggaacagtt
    57541 gttgttcctg ctgagtggga ttgtccaaga tgcggttttc ctgctgggca agataaaaac
    57601 aacccaccat caccaatcaa gaatgagcct tataagaccc acttagctta tgtaaaagag
    57661 cgtcgcacag aggccgaagc taagaaaatt ttggaagcag cgctaaagaa actacgcgct
    57721 gaggatgaag attaaatctt ttctgctgcg ctaagaagtt ctgcaatacc ttgctttcga
    57781 ttctttaagt gaaaacgaat gacgctaaag cctctactgg ataaggcttc cagatcgcct
    57841 agcgcttgag ccataatcaa agtctcaaat ccaaaatctc ttcctggaat tggccaatta
    57901 ttttcattat ctgctgtgac ttgaataaat gaaccagtgc tgggtccacc cttatggaac
    57961 tggccagttg agtgaagaaa tcttggaccc cagccaaagg tggcgggaag ctttgattta
    58021 ctttcaagta gtggcgctaa atctttaatt tgatcatcga tgccgcggtg aagataggcc
    58081 attatcgcta aatagccttt ggagtttgct atagctcttt ttaagtaatg cgccacagag
    58141 tcttcttgaa gtgttgaata aacagcaata ctgccgcttt caaatactgg tttgatttcc
    58201 tctcgccctt tgctccatct ggaaagcagg gcattagtct tatctttagc ctctgtgaca
    58261 tttggttgat taaatgggtc gacttgaagt aaatatccaa gaagtgctgt gacccactgc
    58321 cagaaaataa agtgttcacc aagacttgct tcaacgctgt tttctccact tccatcaaag
    58381 gtaattactg ggtagttaag cttgcttttc tttgtcaaag ttatgggcaa gacgcctttg
    58441 ccatctttgc cagtcgactc tgcaattagc tgctctatcc actcactcag cccagaaacg
    58501 ttagagccag tatcgagaaa tcctgcaaat gaaaaaaact tatcggccaa agcagctgca
    58561 acttgagtaa ctacggagcc gggcttagca aaggattgtg atgcctcgta cgcatcatct
    58621 aagagaattg agatatcgat acctataagt gcagctggag taagtccata ggcgctaagt
    58681 gcgctaaagc gcccaccgac gtttggatca gcatttacta ctcgtaagtt actttccctt
    58741 gcttgaatat caagagggga tccaggatca gtaattacta caaaatgatt ctttggatct
    58801 aggccctgct taattaactg ctgattggct gcagccatct gagaggctgt ttcaatagtt
    58861 gatccagatt tggagccgat gattatgcaa gacttagata aatcttgatc caaaactttc
    58921 ttaacatgag caggatctgt ggaatctaga atttttaatt cctttttata gacctgcgcc
    58981 atcacctctg gcgctaggga agaaccaccc atgccgcaga gaatgaaaac ttcgtggcca
    59041 aattctcttg accaagcaga gagagcatca agctcaggaa gaagctggcg agatttttct
    59101 ggaagattaa cccagttaag tcttatcgct gcctctttag cagcgctctc gccccagatt
    59161 gttggatcgc aattggcaag gcgaattgtg gctttatcta acttctccca aaggcccctg
    59221 ccttgcttaa agccagtgag tttgatcatt tactagcagc ctcaacattt gcaattaact
    59281 caagccaagg tttaataaac ttatcaattc cctcaacctc caacttctct gccacctctt
    59341 caatatctat tccgatactt gctaattgat tgatgactcc agaagcatct aagtattttc
    59401 ccgagatggt atttcctttg aattctcctt gagctttaac ggcatttaaa gtctgctccg
    59461 gcattgtgtt gacacaaaat ggagcaacta gctccattac atattgggtt gggtcatagt
    59521 ttggatcctt taccccagtt gaagcccaga gcggcctttg gatattgcct ccaagttttg
    59581 caagcacttg ccagcgatct gagtgcaaga ccttttcaaa gtgctgatat gcaaggtgcg
    59641 cattggcaat tgccgacttt ccaagcagat ctaacgcctc ttttgaagca ctggctttaa
    59701 gtcttggatc aatttcggta tctacccgac tgatgaagaa ggatgcaact gagtgaatat
    59761 cagagagcgg aagtgatttt cttgcccgat cctctaatcc ctttatgaat gcatctaata
    59821 cttgctcata gcgcttagtt gaaaaaatta ttgtcacatt gacgcttatt ccctctgaaa
    59881 ttagagtttg gatagcggga aggccttcaa tagttgcggg aactttaata agaagatttg
    59941 gtcgatctat cttcttccaa agatctaggg cttgagaaat agtttttttg gtatcgcggg
    60001 caaatcttgg gtctacctca atacttactc gaccatcaac tccgccagtt gctagaaata
    60061 cttccctaaa tagatcgcat gccttagcaa catcgctgca ggtaagttct gtaataatct
    60121 gctcagccga ataacccttg gcagccaaag cctttaaatc atcgctatag agtgaagatt
    60181 gtgatatcga agttgagaaa atcgcagggt ttgtagtcac accaacgacg gattcttcag
    60241 ttataagtga tttaagggag cgcgactttc cattatcaat cattcgctct cgagataaat
    60301 catcgagcca gattgaggtc ccagcctggc ttatctgagc tagcgttttg ctcatttgga
    60361 actctttgcc atcgattctt ggcaagcttt aactactgcc tccggagtaa atccaaattc
    60421 tttaaacaag gtcggagcag aggctgaagc accaaagtgt tccaagctga tggcaacgcc
    60481 gcaatcacca attaattcat gccagccttg agcaatacct gcttcaacgc taactcgagc
    60541 tttgactgat tttggcatta gtaaatcttt atatccttga tcttgctctc gataccattc
    60601 caggcatggc gcgctaatga ctcgagcttt aattccactt tgagatagct cttcacttgc
    60661 tttgagcgca attgatactt cagagccagt tgcaataatt aaaacatcag gggttgaaac
    60721 atcgtttaat gcataggccc cgcgccaaac ttcagctgtg gagttataga gggttcgatt
    60781 aattaccgga agattctgtc tagaaagaag caaaccagca gttttatccc tctttacgat
    60841 ttgcgaccag caggcaacaa cttcatttgc atcagcgggt cgaattacat caaggcctgg
    60901 aatggctcta agggaggcga tatgttcaat tggttgatga gtaggtccat cttcacctag
    60961 accgattgaa tcatggctcc aaacgaaagt cactggaagt ttcataagtg cagcaaggcg
    61021 aaccgcgcct ctcatgtaat cgctaaagac taagaaagtt ccaccaaagg atcgagcaag
    61081 accatgaagc gctattccat ttaatatcga gcccattgca tgttcacgga ttccaaagtg
    61141 aatgattcgg ccatagggcg aagccccctt cattttggaa gtggaaggca agaatgatcc
    61201 gccaccctca atagtggtgt tattgctctc agcaagatcg gcagatccgc caaataactc
    61261 cggcattttt gcagccagag cattaattac atcaccagag gcttttctag ttgagacctc
    61321 tttatcactt gcaaagacgg gaagatctgc gttccaatta gttggtagct cgcgctttct
    61381 caatcgctca agtagcgctg ctcgctctgg gtttgcgctc tgccactttg aaaaatctgc
    61441 gttccaatta ctctttaact taagacttcg ctcctgaact tttcttacat gcttctcaac
    61501 ttcctctggg aagtagaaat cttgtgcagg aagatttagc tcaatctttg ttgccgctac
    61561 ctcatcagcg ccaagtgcag agccatgtga cttagcagta ttgcgggctt ttggtgcagg
    61621 ccaagcaatc acagttctaa ggcgaatgag agttggctta tctgttacca aaagcgcctc
    61681 attcatagct gcttcaattg ccactcggtc aacatcgcca tctgcctttg cacttacttc
    61741 aattacatgc cagccatagg agcgatagcg catagagaca tcttcagtga aggcataatg
    61801 agtatcacct tcaattgata tgcgattatc atcataaata actttgagat ttcctagctc
    61861 ctgcactcca gcaagagatg aggcttcagc gcttactccc tcttgtagat ctccatcgct
    61921 acagatcacc cagatgttat ggtcaaagac gctctcacct tttggcgtat caggatctaa
    61981 aagccctctt tcatatcttg cagccatcgc cattccaaca gcgttagcaa ctccttggcc
    62041 aagagggcca gtagttgttt caacacccaa ggtatggcca aattcaggat gtcctggagt
    62101 aagtgagccc caagttctaa aagcttttaa atcatcaaga gttaatccat atccagaaaa
    62161 gagtaattga atatatagag ttagcgagga gtggccacaa gagagaatga atctatctcg
    62221 cgcaatccaa tttggatcct ttgggtcatg gcgtaaaaaa cgttgaaaga gggtataggc
    62281 aactggggcc agagacattg cagttccagg atggccattg cctacttttt gaactgcatc
    62341 catagcaagg cccctggcaa ttgctactgc cttctcatcc aaatctatcc aaggcgctaa
    62401 ctctggttgc attacaaccc tttccgggcg atcagattag cgccgagatt actagagatg
    62461 aataagttcc aagcggataa ccacaccgcg atagatccga tgatgtgagc ggcaactagg
    62521 gcttcaggca acttggtgaa atattggata tatccaatgc tgccttgggc aagggcaaca
    62581 attagaaata tctgtatccg cccagataag acctggcgag cctgccctgt aagacctagg
    62641 cgaatcgcaa ttaacaaagc gatgctcact ccaagcagag cgattactag gtctgcatgg
    62701 atccaagcca tagtccgtga atcgagatta aatcgctcag ctgcgctatc gccagcatga
    62761 ggtccgcttc cagtgacaat tgttccagcg attagtacta aggatgttaa taagaggtga
    62821 agccagataa gtttggtaac taatgggatt agagtaattt caattggaga tttgccatga
    62881 gctctctgac gcagcgagag ggctcctgcg ataagaatta ttgatagcag gaagtgagct
    62941 gcaactgttg ctggattaag agcggttaaa actgtaattc cgccaaggac tccttgggcc
    63001 aaaattccta gtatctgaag cgcacctaat cttcttaact cacgctttcc gctctttaaa
    63061 atagtaaaca tcagagcaag ggcattaatg agaagaacga aggtaaggag tcgatttcca
    63121 aattcaatcc aggcatgtag cggagcctct ggttgatcag gagttggggt atatgagcca
    63181 ggggtgcact caggccatgt tgagcagcca agaccagatc cggtgatacg aactaaagct
    63241 ccagtacaga caattgctga ttgagtaaag accattaaag tggagagttt taatagcgct
    63301 ttatcggcga ggggggccat agcccaaccc tagagtgcca atgggcgctt tgaagtggcg
    63361 cttggccatg aattacgcaa cacttgcgtt gtgaaatcca ccctagcccc atctgaggac
    63421 ctaagaacta aggaccaggt tgcccgagcc attttagaaa acggcccatc aacggcagtt
    63481 gccttagctg cctctcttgg cttaacccct gctggtattc gccgccattt agattcgcta
    63541 gtgagcgaag gaattctaga agagcgcgaa ccccatatta gaagcgcagg tctaagaggt
    63601 cgcccttcta aggtatttgt gatgagcgat aaagggcgag agagatttga gcattcatat
    63661 gatgatcttg ctgtctctgc gctgaaatat atggcagcta gcgcaggggc gcatttagtt
    63721 gatggctttg ccaagcatcg cgctgatgaa attattcgca gatcaaagaa aactgtaact
    63781 ggttcaaaga atgctgttga tgggttagta aaattcttaa gcgatgaagg atatgcagca
    63841 aatgttcacc caaaagcaat tggtcttgag ctctgccaac atcactgccc gatagcccat
    63901 gttgccgctg aatatcctca gttatgtgag gaagagacag cagctttttc aaagatatta
    63961 ggaacccatg ttcaacgcct agcaacaatt gcccatggcg atggagtctg cacaacattt
    64021 attccccaac taggaagtaa aggcaagaaa atcgaaaaaa cccaaaccag caatagaaag
    64081 cagagcaaac gatgagccaa atcgcccacc cagagttaga gggaatcgga aactacgagt
    64141 tcggttggtc ggataaaaat gatgccggcg ccaacagcaa acgtggttta aacgaagagg
    64201 tagtacgcga tatctctgct aagaagaatg aacctgcttg gatgcttgag ctacgtctta
    64261 agggtttagc gctctttgat aagaagccaa tgccaaactg gggatcagat ttatctggga
    64321 ttaagtttga cactattaaa tactttgttc gctccactga gaaacaggca acttcctggg
    64381 aagatctgcc agaagatatt aaaaatacct atgaccgcct aggtattcct gaggctgaaa
    64441 aacagcgcct agtatcagga gttgcagccc aatatgaatc agaagttgtc tatcactcaa
    64501 tccgtgagga tctagagaag caaggggtta tctttcttga tactgatact gccctaagag
    64561 agcatgaaga tatcttcaaa gaattctttg gctcagtaat tccagtcgga gataataagt
    64621 ttgcagccct taatacctca gtctggtctg gtggttcatt tgtttatgtg ccaaagggcg
    64681 ttcatgttga gattccactt caagcatatt tccgaatcaa tactgagaat atggggcaat
    64741 ttgaaagaac tctaatcatt gccgatgagg gttcctatgt tcactatgtt gagggctgca
    64801 cagctcctat ctactcatca gattctctcc actcagctgt agttgaaatt atcgtgagaa
    64861 agaatgctcg agttagatac accacgattc agaactggtc gaataacgtt tataacttgg
    64921 tgaccaagcg cgcaatttgc gcggaaggtg ccactatgga atggatcgat ggaaatatcg
    64981 gatctaaggt aactatgaaa tacccagctg tatttcttat gggcgagcat tcaaagggcg
    65041 agactctttc aattgctttt gctggagaag gacaacacca agatgcagga gccaagatgg
    65101 ttcatgctgc gcctcacact tcatcaacaa ttatctctaa atcagttgca cgtggcggag
    65161 gaagaactag ctatcgtgga ttagttcaaa tcaaagaggg agcgcatcac tcaaagagca
    65221 ctgtgaaatg cgacgctctt ctaattgaca caatctctcg ctccgatact tatccctatg
    65281 ttgatgtccg cgaagatgat gtgacgatgg gacatgaagc aactgtttca aagattagcg
    65341 atgaccaact cttctatcta atgtctcgcg gactagaaga agatgaggct atggcgatga
    65401 ttgttagagg ctttattgag ccaatcgcaa gagagcttcc aatggaatat gccctagaac
    65461 ttaaccgctt gattgagatg cagatggaag gttcggtcgg ttaatgagca gcgttcttcc
    65521 aactaattac ttaacgccaa ctgggcgtga ggaggcttgg cgctttactc ccttaaagcg
    65581 cattgcaggc cttcatgatc cagcagttag cgttattgat cgcatttcaa ttgagttatt
    65641 cggaagcgct agagctggtt ttaatatctc aactgttaag gcaagtgagc ttcccgcaaa
    65701 gtcagagaca actgatgcaa ttgttcagcg cctaaggagc gaagttactg aagttcttca
    65761 tctaaaaatt gataatgaag cagtaattaa cgaaccnnnn nnnnnnnnnn nnnnnnnnnn
    65821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    65881 nnnnnnnnnn nnnnnngcat aaatggaatc tatcgagata aaagtagatc aacttcaaga
    65941 atcaaagccg gttaagttca cacttggttc agaggatgtc tgtgttgcaa gaattggcga
    66001 tgaagtcttt gcagtcgctg atatctgtag tcatgccgag gcatccctct ctgaaggaga
    66061 tattaaggat tacaagattg agtgttggct ccatggagct gagtttgatt taagaagcgg
    66121 agaggtttta actccgcctg cttttgaagc aattgaaaca tatgcagtag ttcgtgatgg
    66181 agatactgtc acgatatcaa ggaagaaata acgttaagga gtaatttgcg atggcacaac
    66241 tagtaatcaa aggtcttcaa gtaagcgttg agggcgagag tggagctatt gagattctcc
    66301 gtggggttga tctaacgatt aactctggtg aggtccatgc aattatgggc cctaacggct
    66361 ctggcaagtc aacacttgca tattccattg caggtcatcc taaatacagc gttacaggag
    66421 gatcagtaac acttgatggc gttgatgtcc ttgaaatgag cgttgatgag cgagcaaagg
    66481 ccggactctt tcttgcgatg caatatccgg ttgaggttcc aggagtatct gtttcgaact
    66541 ttctaagaac tgcagcaaca gctcttcgcg gcgaagcgcc taaggtaaga acttgggtag
    66601 gagaggttaa agaggcgatg agcaaactat ctatcgatcc tactttttca gagcgcagcg
    66661 ttaatgaggg attctcaggc ggagagaaga agcgccatga gattttgcag atggaactac
    66721 tcaagccaaa gattgcaatc ttggatgaaa cagattctgg acttgatgtt gacgcgctaa
    66781 gaattgtctc tgagggagta aatcgcgtaa agagcgaaca gaatcttgga atcatgttaa
    66841 ttactcacta caccagaatt cttcgctaca tcaagccaga cttcgttcac gtctttgctg
    66901 gcggaaagat tgttgaagag ggtggacctg aacttgctga tcgacttgaa gagaagggct
    66961 atgcgcaata cgcaaaggcg taggcgataa cttgtgtcta aattaaatct ctcagcgata
    67021 agggcggact ttcctatcct taagcgagag attcgtggca acaatcgcct catctattta
    67081 gatagcgggg caacttcaca gaagccaaac tcagttatcg cagctgagcg agatttctat
    67141 gaaaaccata acgctgcagt tcatcgaggc gcacatcaat tagcagaaga ggcaacagag
    67201 ctctatgaag gagctcgggc aaaagttgct gcctttatca atgcagatgt cgatgaagtc
    67261 atattcacaa agtcagcaac tgagagcatc aatgcaattg catattcact tggaaatgct
    67321 aggccagggt ctaagtttca tattaagcct ggtgatcgaa ttgttgtctc tgagatggag
    67381 caccatgcca atttaattcc atggcaggag ctatctgctc gaaccggagc tgagttagtt
    67441 tggtttaagg taagtgataa tggccgatta gatctatcta atatggatga gctaattaat
    67501 gagagaacga aagttgttgc ccttactcat caatcaaatg tgcttggaac tatagttccg
    67561 ctggatcaag taacagcaaa ggcacatagc gttggcgctc tatttgttct tgatgcttgt
    67621 caatccgttc cacattacaa agtcgatgta aaagctttga atgttgattt cattgccttc
    67681 tctggccaca agatgcttgg cccaaccgga gttggaatcc tctggggaaa gaagaacttg
    67741 ctagatgaga tgccaccctt tctaaccggt ggatcaatga ttgaaactgt aacaatggag
    67801 agagcgacat atcttgacgc tcctaaacgc tttgaagctg gtgtgccaaa tatggcgcaa
    67861 gcaattgggc ttggagcagc agttgattat ctaaacgcca ttggtctgga tgaaattcac
    67921 gcccatgaag ttgagttaac taaggcagca cttgatggct tgcagagtat taaaggactt
    67981 tcagtaattg ggccaaaaga tttagagatg cgcggaggag tagtctcctt tgctgtcgaa
    68041 ggagtgcatc cacatgatct tggtcaagcc cttgatcaat atggcatcgc ggtaagaacc
    68101 ggccatcact gcgcatggcc actaatgaga cgctttaaga ctgttgctac cacgcgagct
    68161 tctttctatc tatataacga ctttgatgag attcaagctt taattgatgg agtggagcgc
    68221 gcaagaaagt acttcgagag ccgataatgg aactagattc tctttatcaa gaagttattt
    68281 tggagcacta taagcgccca cttaataagg tcttaaagcc aaatccaaca attcaagttc
    68341 aacatataaa cacctcatgt ggcgatgaga ttactttaaa tctgcatact gatggcaaga
    68401 aggttataga agttagttgg gagggaatcg gctgctcaat ctcgcaagct agcacttcag
    68461 tagtatcgga tttagtgatg ggcaaagaaa tcgctgaggt cctaaagatt atcgattcat
    68521 tccagggcat ggtcttaagc aaaggaacag attctggcaa tgaagagcta atcggagatg
    68581 gcgtagcttt tgctggagtt tctaagtttc cagctagagt aaaatgcgct ctattgggat
    68641 ggatggcaac gaaagatgca atacttcaag caacaagtag cgaagggaag tagatgatga
    68701 gcgcatcgag agatgatgta acagaggcaa tgaaggatgt tattgaccca gagctaggaa
    68761 tcaatgtgat tgatctaggc cttgtctacg atgtctctat cgatgattca aatgtcgcag
    68821 tacttgatat gacccttacc tctgcagctt gtccattgca agatgtgatt gaggatcaaa
    68881 ctaaatcagt gcttcaagat ctagtaactg atgtaaagat aaattgggta tggatgccac
    68941 catggggacc agataaaatc accgatgatg ggcgtgaaca gcttcgcgca attggcttta
    69001 ctgtctagtt cgttcctctc cttcgcgatt ctggtctagg gtagagatat gtcccaaatg
    69061 gcagtttgga tgtcctttcg ttccttcaca gccgattctt cagttaagaa ccagaaacta
    69121 aagccaggaa ctgtaaagag aatctttgga ttcgctcttc cttataagag ctttttaatt
    69181 ttctttttaa taacagttgt catcgatgct ttcttaattg tgactactcc actacttctt
    69241 agaaatctaa tagatgatgg cgtcatacca ggcaattctg ccttggttac tcagatagca
    69301 cttttggttg ccttcatcgc aattctcgat gccctcttta acatgattgg tagatggttt
    69361 tcagcaagaa ttggtgaagg actaatcttt gaccttcgct cgcaagtctt cacccatatt
    69421 caaaagcaat caattgcttt ctttacacgt actcaaaccg gagcattaat ctcaagaatt
    69481 aactctgatg tcattggggc tcagcaggcc ttcacctcaa ccctttctgg gctaatcagt
    69541 aatgttctaa gtttgattct ggtaactgta acgatgttaa ttctctcctg gcagatcact
    69601 ctggtttcat tattactgct tcctgccttc attattccga ctaagtgggt tggcaagaag
    69661 cttcagggat taactaggga ttcatttaac ctaaatgctg aaatgtcagc aactatgacc
    69721 gagcgcttta acgtctctgg cgctttacta gtttcacttt atggtaatcc aagtagtgag
    69781 agttcaactt ttagcgccag agctagaaaa gtagcaaaca ttggtatttc aatcgctcta
    69841 ttaaaccgac tcttctttat cgcgctaact tcaattgcag caattgcgac tgcttttgcc
    69901 tatggagttg gtgggcattt cacaatcaat gagcagatat cactaggaac tctgctggca
    69961 atcactgcgc ttctagcacg tctttatggt ccactaacag cgctatctaa tgtgcgcgtt
    70021 gatgtaatga ctgctttagt ttcatttgag cgcgtatttg aagtccttga ccttcagcct
    70081 atggtctctg ataagccaga tgctaagcca gttggcagtg gggggctgag catcaaattt
    70141 gattcagtcc gtttctccta tccaagggct gaggagatct cacttgcctc tctggaatcg
    70201 gtagcaaagc ctgagacagt tgatagtggt gaggtattaa aaggaatttc atttgaagtt
    70261 cttccttcaa caatgactgc tttagttggt agttcgggag cgggaaagac gacgattagt
    70321 gcgctgctac cacgtttata tgatgttacc tcaggctcaa tcgcaataaa tggggttgat
    70381 atccgggatc tgcaagttca atcacttcgc tcaagcatcg gggttgttac ccaagatgcg
    70441 catatgtttc atgacacgat agaggcaaat cttcgatatg caaaagttga tgccacaaca
    70501 tctgagatgg agaaggcttg tcaagatgct caaatacttg aactgattaa ttccctacct
    70561 aatagatttg aaaccgttgt cggggagagg ggccatcgac tctcaggcgg cgagaagcag
    70621 agacttgcca ttgcacggct gcttttaaaa tcacctagaa ttgtaattct tgatgaagca
    70681 acagcccatc tagattctga aaatgaattc ttggttcaag ctgcgctagc aaatgcttta
    70741 aaggatagaa cgagtattgt tatcgctcat cgcctctcaa caattagagc tgctgatcaa
    70801 attttggttc ttgataatgg ccttattgca gagcgcggca aacatgatga actcttggcg
    70861 ctaaatggaa tgtatgccga gctttactct cgtcaaagct ttggcgcttg agtttttcga
    70921 tcctcacgaa ttacgagata ccaagcaaag agcactagga atataaagag tatccactgg
    70981 aatgagtaag ccagatgggg tccatcgctt agttctggca gggcagttgc aacctctggc
    71041 gtcacttcag gatttgaact agagattaaa tcaaagtaga tgggctctgt ttcaatagct
    71101 tgttccgagt tccacttaac taactttggc gttgagtctg ctccaggcag agcaaagacg
    71161 ctgccttgaa ctcttgactc aatctcagag gttctgactc tggcggtgat ttcaacaggt
    71221 agtgaatcaa ctttttgaac aactggggga gtttgggcat ctttgccagc tatcacccat
    71281 ccgcgatcaa tccaatatct cttgccacta tcagagataa atagcgtgat tacaccaaaa
    71341 ccatatttac cctcgtgata acgatttcta atcaggaact ctctgcttgg atcaaagttt
    71401 ccaacaagtg agatacttcg ccaagcaatt tctgaagctg ttttactact taagtcatct
    71461 tctgtaagta gagcggcgat agaaacgttt ttccggatca attcgttatt tgcatggcga
    71521 aattcatatc tctcatattg ccataaagcg ccttgcacac agccaaagat aagtagtaga
    71581 gctagggttg agacggtaat taggcggcgc attaaagcaa tcttagactt aacctatttc
    71641 gcgttgtttg cttcttaata ctgcgctctt gcctctaatg gtctccctgc caccatttgc
    71701 cataatcact gagatgtagg gaagggttac tgctccaact agaaagaacc agcgataagg
    71761 acttggcgta aaaactgcta ggagaaaaca agcagttcta atcatcatcg aaatgaaata
    71821 gcgcttctgt cttgatggaa gatcatcaga gagggcagcg cctgcctcgg taatgttttg
    71881 ataattgcgc ctcttaggca tcgctagatt ccaacgcttt aaggccatag ctaagcgtag
    71941 atgggataga ccataagcgc attttgacct tcattttcgc actcgctagt atctagccct
    72001 tggtcaaatg accaacatga agtggagcta gccgctccca cctacctcac ttttaggtga
    72061 gctggtcaac aagagcaaga ccaaaatatt ggtctcattc tggcggctga cttccatcat
    72121 gcttttagtg aagggaagag ttatcagcac cactgatcct cgtatcaacg atcgaattcg
    72181 cgttccagaa attcgtctga tcggagcact aggtgagcaa gttggcgtcg tagcgataga
    72241 taaagcgcta aagatggctg atgaagcagg tcttgattta gtcgagattg cacctgaagc
    72301 agttccacca gtatgcaaga ttatggattt cggaaaatat aagtacgaaa ttgctcagaa
    72361 agcgcgagag gcccgggcta accagaccca cattgtggtt aaggaaatcc gaatgggttt
    72421 aaagattgag aaccatgatt acgagactaa gaagaaccat attgaaaagt tcttgcttag
    72481 cggagataag gtgaaggtaa ctgttcaatt caaaggacgg gaacagacaa gaccagagat
    72541 gggatttcga ttattgcaac gtcttgcaga agatgtagct acttcgggat ttgtcgaatt
    72601 tgctccaaag cgcgagggta gaagcatgac gatggtgctt ggaccagtgc gcaaaaagag
    72661 tgaagcactt gctgaagcaa agcgcgcccg cgcctctaag gctaaagaag tttaagtaaa
    72721 gacaacagga ggaaagaaat gccgaaaatg aagacacata gtggcgccaa gaaaaccttt
    72781 cgcgtcaccg gaagcggaaa gatcgtgcac gagcgcgctg gcaagcgcca cctcttggag
    72841 cgtaaatctt ctcgagtaac ccgtcgcctt tcaaaggatc agacggccaa tgatcgaaac
    72901 actttttctg ccaagcgttt gcttggaatg aagtaagggg gagcaataat ggcaagagta
    72961 aaacgcggtg ttcatgccgc aaagaagcgc cgcaccacac ttgagagagc tagtggctat
    73021 cgcggacaac gctcccgtct tttcacaaag gcaaaagagc aagtaactca ctctctggtc
    73081 tatgccttca atgatcgtaa agataagaaa ggtgatttcc gccagctctg gattacccgc
    73141 atcaatgcgg caagtcgcgc taatggaatt acctacaacc gttttatcca aggattaaag
    73201 tctgcaggag ttgaggtaga tcgtcgcgtt ctatcagagc ttgcaactaa tgatcctgct
    73261 gctttcaccg cattggttga agtagcgcgc aagaatgtaa aagtttccta aagtctcgcg
    73321 ttagaaagcg atgattacga gccttcattc gcctcatgtt gaggcagtga aggctctttt
    73381 aggttctcgg ggcggcaaag caagaaaaga gagcggtcaa tatgtaattg aagggctctc
    73441 atcaattaag gaggcccttg atttttcgcc tgaagaaatc acaaccctct atctaactag
    73501 cgatggcatg agcagacttg caacaattcc tgagggctat tttgaaattg ttgaggtctc
    73561 acctgaagtc atgaaagcaa tgactgatac cgtcacaccg caaggtttat tagctatcgc
    73621 tcagattccg cagaattcat ttgaagattt ccttgcagcg tcaaagagtg agttaaagat
    73681 tgcttacttc tggcagattc aagatccagg aaatgctgga acggtgatcc gagctgcaga
    73741 tgcttttggt tttgatgcag ttattttctc taacaatagc gttgatatct atagcccaaa
    73801 agtagttcgc tcaagtgcag gttcacactg gcatattcct ctatttacat caatttcaga
    73861 aggagaacta aaaacacagc tttcaggcaa ggcctttgat atctatggaa ctgatgcaag
    73921 tgggggactt gaactattag aggcagctaa tgaatcaaag aatcgctctg ctatctgggt
    73981 atttggcaat gaggcaagag gccttgatgc gaaggtgatc tcaactcttg gagctaagca
    74041 agtagcaatt cctatttcag gcaaggcaga gagcctaaat ctcgctaccg ctgcctcagt
    74101 ggtgatgtat gccgtatcgg cagctcgaaa ataaccactt cttgccactc ggattagtag
    74161 aattctctag tgtcgctaga taacgcaggt attgagcaga tcaaagccga tgcgctatct
    74221 gcaataaaaa actcccaatc cctagatgag ctgcgtgagg taagaattgc tcatgtcggt
    74281 gatcgctctc cgatagcaag ggctaatcaa ggacttgcag agattgagat tgaaaaacga
    74341 gcagcgatgg gaaaattgat tggaactgct cgggccaata tcaaccaagc atttgatgat
    74401 aaagaaaaag agttaactgc gctaagagat agtagagctc taagcaatga aagagttgat
    74461 gtaacccttg ctagcaatag aaatcctcgt ggagcgcttc accccttaac acttctaact
    74521 aatgagattt cagatctctt tgttggcatg ggatacacgg tgcaagaggg gccagagctg
    74581 gaggcttcat ggcttaactt tgatgcctta aatattgccg aagatcatcc ggctagaact
    74641 atgcaagata cattttttat cgagccgctc tcatcaggtc ttgtaatgcg aacccatacc
    74701 tcgccagttc agatcagaac tatgttggaa aataaacctc caatctatgt gatctgtcca
    74761 ggtcgtactt atcgagctga tgagttagat gcaactcata ccccagtatt taatcaagta
    74821 gaaggtttag tagttgataa gggcataact atggcccatc taaagggaac cctggaccac
    74881 tttgcggcaa agatatttgg tcctgacgtc accacgagaa ttaggccatc tttctttcca
    74941 tttactgagc caagcgcaga agttgatttc ttctataacg gtcgctgggt tgagtggggc
    75001 ggctgcggca tggttaatcg aaaagttttg caagcggcag gaattgatac cgatatttac
    75061 actggatttg catttggaat gggccttgaa agaaccttga tggtgaaata tgggatcaca
    75121 gatatgcatg acatcgttga gggagatgtt cgcttctcaa gacagttcgg aagtggtggg
    75181 cgatgaagct tccactatct tggctctcag agtttgttga gttccctaaa accatttcag
    75241 tagcagatat cgctgctggg tttgtaaaag tgggttttga agtcgagtcg attgataatc
    75301 cagccgagaa aatcaaaggt ccactacttg ttggaaaagt attaactatt gaggagctaa
    75361 ccggacataa aaagcccata cgctatgtcg ggcttgattg cggggaagct aaaacaagat
    75421 atgtaatctg cggtgctact aattttgttg ctggagatct agttgtagtg gctctacctg
    75481 gtgcgatcct gccaggaaac tttgcaattt cagcccgtga aacatatggc aaaaccagca
    75541 atggaatgat ctgtagttca aaagaacttg gcctaggaga tgaccactca ggaattatcg
    75601 ttctagatag caaggcaaag attggaagcc cagcacttaa ggttttagga gcagatgatc
    75661 caattattga tatcgcagta aatcctgacc gcggttatgc gatgagcgtt cgcggcgctg
    75721 caagagagct agcaatggcg ctagggctag gcttcaagga tatttcttca aaggccttaa
    75781 ttgcatcact tgagaaaaaa aagaagcggg gaaagataac tcccgtacaa atttcagata
    75841 aaactggagc agatcaaatc tacctgcgga gcctaagtaa tgtagatccg ctaaagccaa
    75901 caccgcagtg gatggcaaga cgtctatctc aagcaggaat gcgcccaata tctattgcag
    75961 tagatatcac caactatgtg atgttagagc tgggtcagcc gctacatgcc tttgatagcg
    76021 ccaagatttc tggaacttta agagttgcaa gagcaggaaa attcactgaa ttgataaccc
    76081 ttgataaggt caagagaaag cttgctaagg ataatcttct aataacagat gataaaaaag
    76141 tactagctct agctgggact atgggcgggc ttgatagcga agtaagtgag tttactgttg
    76201 gcatcacttt agaggcggct cactttgatc caatgtcaat tgcgcgtaat tcaaggagcc
    76261 atattctaag ttcagaggct tcgcgtcgat ttgagcgtgg agtagatcca ttgcttgctg
    76321 agttggcttc tgctcgcgca gcgctactac ttatcgaata tgcaggagct atctataacg
    76381 gaagctcagt aaagaaaact ccgctaaaaa agagaagcat cactatttct gcctctgaaa
    76441 tttcatcact tataggaaca acttatagcg acaaagaaat tgaaaaatca ctaaaggcga
    76501 ttggcgctag cttaagaaag agtggaaaga aatgggttgt taccccgcca tcctggcgcc
    76561 cagatctaaa cgaacttcca gatctggcag aagaggttgc tagattcttt ggatatgacc
    76621 gcattccttc tcaacttccg ccagtgaaat tggcaagtaa tggaagttca ggacttaccc
    76681 ctatgcaaaa gcgtcgcaga tcaatgagtt tgaaattggc caatagaggg ctggtcgaag
    76741 ttcataacta tccttttgta agccaatccc aaatggatct ctttggtttc actggagatc
    76801 gcgcaaagac tttcaagatt gctaacccaa tgtctgatga atatccctac ttaagaacac
    76861 atctaacccc tggacttcta gctgcagctg gaagaaatct atctagggga gaaaaatcgg
    76921 tagcactctt tgaaagtggt gcgatattta gaaacattga aaccttaaag ccagcaggaa
    76981 gtgtttcagt cagtaaaaga ccgacggatt cgcagataaa gcagatttat cagagcgtac
    77041 ccaagcaacc tcttcatatt gccggagtaa ttgcgggaga gattacaaac tctggttggt
    77101 ggggcaaggg aagagttgtt gagtggagcg atgccgttga tctagtaaga gagttgattg
    77161 aagattctgg aaatgattat tcaattgaag cagttgatct tgcgccttgg caccctggaa
    77221 gatgcgctga attcagagtg gatggaaaag ctgtggcgca tgccggccag attcatccac
    77281 gagtaacatc agcgcttgct cttcctgatg caactgtggc atttgctctt atcgttgacg
    77341 ctctaaaatt tccagacaat tttaaggcca gtccaataag tgttatgcca gctgcaatcc
    77401 aagatatctc cctctttgtt gaccagaagg tgccagcaga aaaagttgca gccgcccttc
    77461 gagagggcgc cggtgaacta ctggagagca ttgcgctttt tgatagatat cagaaggagg
    77521 gagaggctca ggtttcgcta gctttttctc tagtctttag agcctcagat cgaactctta
    77581 cctctgatga agtttcagaa cttcgcgcaa aggctggggc gatggccagc aagaagtgcg
    77641 gagcccaaat ccgcacctga tgcataaata tgcagggatt tgcataacta tgcaccaaag
    77701 gctaacctgc ctccatgaaa atcggagtcc ttggggctag cggctatgcc gggggcgaac
    77761 tgttgcgttt gttatctggg cacccagatt ttgaagttag ctacatcgcc gcaggttcaa
    77821 atgcggggga gaagattact gatctccatt cacatctaat tacttatggc gatcagagat
    77881 tcagtggcac agatattggc gcaattaata actgtgactt gatttttctt gcccttcctc
    77941 acggccaatc tgctgagata gttaagcaga tagatgaaaa ggtaaaggtc gttgacttag
    78001 gtgccgattt ccgtttgaag tcagagcaag cttggaaaca gtattacaac gatagttatg
    78061 caggagcttg ggcttatgga ttgccagagc tgcctggaaa gcgtgagtta ataaagaagt
    78121 cttcaagggt tgcaaaccct ggatgctacg ccaccgctat agcactggca gccgctcctg
    78181 ccgtgctggc aggtggagta gatgcagggg acattgttgt tgttgcagca agtggaacaa
    78241 ctggagcagg aagaagcacc aaggtaagcc taagtgctag cgagattatg aattcgatgg
    78301 tctcttataa atttggcgga gtacatcaac atactcctga gattgaacaa acgcttgcag
    78361 agcttggttc aaaagaagtg aaaatttcat ttaccccaat tttggcgccc atgccaagag
    78421 gaatacttgc aactgtcact gccaaaacaa ctctttcaga ggatgagatt cgtaaaagct
    78481 atgaaaaaat gtatcaagat gagccatttg ttcatttact ttcaaaggga caacttcctg
    78541 aaacttcttc gcttactgga tccaattcag ttcaaatgca agtagctgtt gatgcccaca
    78601 catctagatt aatcgtctct gttgccattg ataatttagg aaaaggggcg gcagggcaag
    78661 ctattcaaaa cgctaattta atttcaggac ttgttgagac aactggacta tcagcattgg
    78721 ggatagggca atgactctaa agagtgaata tagccagagc cttcccaagg gctttagggg
    78781 atctggagta agtgctgggc ttaaaagctc tggggctaag gatctagctc tcattgttaa
    78841 tgatggacct gataattttg caagcgcagt ctttactacc aatcaagttg tagcagctcc
    78901 tgttatctgg agccggcaag ttctcaagga caatcaagtt tctgcagtct tactaaatag
    78961 tggtggagca aatgccgcca ctggagccga tggctttgct gatacacaca aatcagcaga
    79021 agaggtagct agtttgttat cgctttcatc ctctgatgtt gctatctgtt ccactggatt
    79081 aattggcgta aggcttgata tggagaaatt acttgcagga gtcaaaagcg cccactctgc
    79141 tctgagtact gattcaaccg aagatctagc aagagccatt atgactacag attcaaaacc
    79201 aaagatcgct acctatgaga gctcaggtgt tagttttacc ggaatagcca agggagccgg
    79261 gatgcttgcg ccatcacttg ccaccatgct ttcggtagta atgactgatg caaaagtcga
    79321 taaggcagtg gccgatgaga ttttcgcaaa agtttgtgag aagactttta atcgaattga
    79381 ttcagatggg tgcacatcaa ctaatgacac tgttctcttt ctctcatctg ctgcctcagg
    79441 agttgagtta tcaaatgagc agcttgagca ggcgctattt gcagttgctg caagtctttc
    79501 aaggcagcta attgctgatg ctgaaggaca ctcaaagatc atttcaatca ccacgataaa
    79561 tgctaagagt gagagagatg ctgttgaggt gggcagagca tgtgcaagga ataaccttct
    79621 taagtgcgca cttggtggag aagatcctaa ttggggtcga gttttagcag cgattggaac
    79681 aaccaatgcg gtaattgatc cactcaatat tgatgttgat ctaaatggag taaaggtttg
    79741 taaatcaagc tcgccagggg aggatagagc taaggtagtt atgactggag aattgataga
    79801 gattctgatt gatttaaagg taggcagttc ttctgcgact atctggacca atgacttaac
    79861 tgcctcatat gttcacgaga attcggctta ctcaacatga tcgttattaa atttggcggt
    79921 catgccatgg gcgagcactc cagaaaatgg gcgagtgaga ttgccagccg cttcaaattg
    79981 ggagagagat ttgtcattgt ccatggcggt ggaccacaga tagatagaga acttgcaagg
    80041 cgcggaatag aaaaatctag cgtcaatggc tttcgcatca caactccgga aattatggag
    80101 gttgttgaat ttgtccttac cggaagcgtg cttcgctcag tagttagaga tttaatcgct
    80161 gctggacttc ctgcagttgg tatcacagga agtgataacc aattactaga ggttgaatta
    80221 agggatgagg caaagtttgg cctggttgga aagattaaga gagtgaatcc aaagatcatt
    80281 aatgatttac tagatatggg atatttacct gtcatatctc cagtggccaa tgacagttca
    80341 actagggccc ttaatgtaaa tgcagatatt gcagcagggg ctattgcagg ctcacttcga
    80401 gcaaatgagg ctctttttct taccgatgtt ccgggaatct atagcgcatg gcctgatcgc
    80461 tcctcgttga taagcgaaat tactatctct gatttgaaga agatctcatt tgagggtgga
    80521 atggttccaa aggttgaagc ggttattaac gcgattgagt ctggagcatc atctgcaagg
    80581 gttattgatg gcacatcact tccagcattt gttgatgcct taagcggcga tggtggaacg
    80641 ttggtaaagc catgaagaaa ggtaataaag aattactgaa aaaatggaac tctgtaatgc
    80701 agagcaatta tggaacccca agcatctctc tttcaaaagg taaaggcatt gaagtctggg
    80761 atttagatgg ggtcaaatac ttagatttct tgggaggtat tgcaacaaat cttcttggcc
    80821 ataaccatcc agcgatagtg gccgctgtct caaagcagat taggcaatta agccatgtga
    80881 gtaacttcta tgcacatcct gtggcgctag agcttgctga gaaacttcag aaaatgacga
    80941 cagataagag cgcaagagtt ttcttctgca attcaggagc tgaggcaaat gaagcagcta
    81001 taaagattgc tcgtcttcat cgcggcaaaa gaatagttgc cgcaactggg gcatttcacg
    81061 gaagaaccat gggagcactt tcgctaactg gacagagttc aaaaagggat gctttcaagc
    81121 cattacttcc tggagttaag cacgtgaaat atggtgacat taaagcctta agaagggcag
    81181 ttaataagag aacttccatg gtaattcttg agcccatcat gggcgaggct ggggtagtga
    81241 ctcctccaag tggctactta aaggcagcaa ggcagatctg tgatgaaaat ggcgctctgc
    81301 ttgcaataga tgcagtgcaa actggtatgg gtcgctgcgg aagttggttt ggatatgaag
    81361 atgaaggaat aaagccagat ctaatcacaa ttgcaaaggg cctaggaggc ggacttccat
    81421 tggcagcaat gatcacgtta ggcaaagctt ctgaactttt taaacctgga tcacatggca
    81481 gcacctttgg aggaaatcct gtagcagcag cagcagcgat tgcagttatt gatgagattg
    81541 agaagaagaa atatctggcg ctaaatactg taaaaggagc gctaatacga aacttgatct
    81601 cgccaattat tggcgtcgag caagttcgag gcaaaggctt actaataggt atttgtgtta
    81661 aggaaagtaa agctaagcag atagcagccg aaatgcaatc taaagggttt ctagtaaatg
    81721 ctgccagcga tgatgtaatt agaattgctc cagcttacgt agtaagcgaa aagcagatta
    81781 tgtcttttgt ctctgctttt aattcaagtt gtgaggagat ttatcgtggc taaacgtgga
    81841 actctctcat ctaatgcaag gagagcgctt gtagtcaact ttgttaacca aggacttgtg
    81901 cattcgcaag gtgatttagt agagcttttg gctgaagaag gtattgaagt aacgcaggca
    81961 actgcaagtc gagacctaga tgatgttgga gcggttagag gtaaagattc ctctggagtg
    82021 atgcgctatc aatttctcga ttcaagcgtg gagcccttgg ccagaatggc ccgagtctct
    82081 gatcaacttc tcttaagtat caccgctagt ggaaatatcg tggtcataaa gactccacca
    82141 gggggagcgc aactactagc tagcgccctt gatcgcgctt cggagagctc agagctttca
    82201 agtgcaatag gaactattgc aggagatgac acagttttag ttatttcacg aaacgcaagc
    82261 ggcggaaagc ttctcgctgc cgagcttcgt gattacatta ccactgagtt ttcaagtaac
    82321 gctcggaaaa cgagcaagtc aaagaaagcg agacggtaat ggcgctttgg ggcggcaggt
    82381 tctcaaaaga ggctggagag gcgatgtttg cactctcaag gagtgtgcac ttcgactggc
    82441 gccttgcttc ctatgactta agaagttcgc tagctcacct taattcttta gaggcaagtt
    82501 caataattga taaagcacta gcgctcaaga taagaagcgc aatcaaagag ctgcaaagtg
    82561 aagtagcaag tggcaaattt gctccgattg ctagcgacga ggatgtccat tcagcacttg
    82621 agcgaggact tatagaaaaa cttggcgcag aaggtggagc aattagagct ggtcgatcac
    82681 gaaatgatca agtagctacc gattttcgcc tttacttaat tgatcacatg attgaaattg
    82741 cgctagagac tctaaaactc agtagcgcta tcaccgattt agcaggtgaa tatgcaggcg
    82801 atgtagcacc tggctttacc catcttcaac atgctcaacc agtttcattt ggtcatgagt
    82861 tagctaaaca tgctcacagc ttaagtaggg atgtttcaag aatctcagat tggctgactc
    82921 ggagtaatct ctcgcctctt ggcgcaggcg cgctcgccgg atcatcactt ccactagatc
    82981 ctgaatcaag tgctaaagag cttggattta gcggagcaat agcaaatagc attgatgcag
    83041 ttagcgatag ggattttgtc gcagaagctc tctttgtcat ttcgttaatc ggtattcatc
    83101 tctcaagaat cggtgaggag tggagccttt atgcaagtag tgaatttgct tgggttgaat
    83161 tagatgatgc ctatgcaaca ggctcttcga ttatgccgca gaagaaaaac cccgatgcag
    83221 cagaacttgc acggggaaaa gctgggcgat ttattggttc actcactgga ttattaaccg
    83281 ttctaaaggg tttgcccttt gcctataacc gcgacttgca agaggataaa gagctgacat
    83341 ttgatgcagt agaaaatctc ctgctattgc ttcccgcaat tactgggatg gttgcaacta
    83401 gtaaatttga tagagagaaa atgagagcag ccgcgcccct tggtttttct cttgccactg
    83461 agattgccga ctttctagtc cttaaaggag tggcttttcc tatcgcccac gatgccgcag
    83521 gtaaagcggt tagaagatgt gaagagcttg caattgaact tgatcaatta agtgatttgg
    83581 atcttaagag tattcatcca ctcttaagtg gcgaggttcg aagccgtctt aatcttgatg
    83641 cagccttggc tgctagagct agcgcaagtg gaaccgcccc tgagagcgtt aagaaacaga
    83701 ttgctaattt acgagtgaaa atttctgctg acgctagcga atttgccggc gagcggaaga
    83761 gattttcagg catgatggac caatgagcaa agcgctactt gatgatttaa cttggcgcgg
    83821 ctctatcgcc cagagcacag atagagctga gttagagaag tatctagcta accctggagc
    83881 aagtctttat cttggatttg atccaaccgc tccaagtctt cacattggaa atttggttgc
    83941 tctaacgctg cttcgccgat ttcaattagc tggttataaa ccgatagcac ttgttggggg
    84001 agccacaggt ttagtaggag atccaagtgg gcgaagcgct gagcgaagcc taaatgaagc
    84061 tgatttagtt gctgattggg ttgagcgaat cagaaagcaa ttagagggct ttcttgattt
    84121 cacaggtaag aacgcagcga ttatggcaaa taaccttgat tggacagcgc ctgtttcagc
    84181 aattgaattc ctaagagata ttggaaagca ctttagcgtc aatcagatgt tagctaagga
    84241 ttcagtggcc acgcgccttg aatcaggtgg catctcctat actgaatttt catatcaagt
    84301 tatgcaggcc tttgattatc tggaactatt cagaaggcat aactgcgcac tacaaattgg
    84361 tggcagcgat caatggggaa atatagttgc aggtgttgac cttattcgtc gcgtggaagc
    84421 aaagagcgcg cacgccctaa caatccctct tcttgcaaaa gctgatggaa caaagtttgg
    84481 caaaactgca gagggagcaa tctggctaga tccaactatg acctctgcct atgcgctatt
    84541 tcaatttttc ttgaattcag atgatagaga tgttgaacaa ttgttaaaaa cattttcatt
    84601 taagtctcgc gcagagcttg aggcgctctt tgaatctctc aaaacaaatc ctggggcaag
    84661 agaggcccat cgagaacttg ccagagaggt aacaacactt cttcacggcg ccgatcaagc
    84721 aagaaaggta gaagctgcag caaaagctct atttggtcag ggcgagataa gagatcttga
    84781 tattgaaact ttaaagtcag cgctagcaga gcttccaaag gcaagaatta ataagggagc
    84841 agagattcct tcctgggtag atctaattac tagctctgga gtggtggaat ctaagtctgc
    84901 tgcgcgaaga gttatcaaag agggcggggc atatttaaat aatgagaaaa ttaccggcga
    84961 ggatttcgct ccgaagagtt ctgatctgct ccatggccaa tttctcttgc tccgcaaagg
    85021 aaaacgcgat cttgtcgcgg tagaaatcac cgattagagg atttgcaccc cttaaaaaag
    85081 agcgttattc tttgctccct cgctacgaaa tcggacttca acaccaggcc gagtagtcga
    85141 gcaagaactt ccctagaagc taaaaccctt atttatcaag gttttttggc ctctgcgccc
    85201 cattttttgg gtttcgcagg ggcgatttga ccccaagtga ttaaaggcct aagtttctcc
    85261 ttctgccctg taaaaggggc ggattggttg tgccctgcca acggctgtaa ggcccggaat
    85321 cggtgcgcac c
//
LOCUS       DAOROD010000004        69357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_13_length_69357_cov_31.028527, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000004 DAOROD010000000
VERSION     DAOROD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 69357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69357
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_69357_cov_31.028527"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..772
                     /locus_tag="PLW53_01740"
     CDS             116..772
                     /locus_tag="PLW53_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HRD36182.1"
                     /translation="MRRNLAALISLTLTLWILPATTAAAANPKAGAKCSKAKQVKIYK
                     GKSYTCIKSGKKLTWSKGKTISSVENASTPTPTPTPTPTPTPTPTPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTKVGYTMDQVKANNTSASCWTV
                     IDNYVYNLTNWISSHPGGAGAIRSLCGVDGTASFKAQHANQSNPASRLNSYLLGPLSK
                     "
     gene            complement(777..1337)
                     /locus_tag="PLW53_01745"
     CDS             complement(777..1337)
                     /locus_tag="PLW53_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HRD36183.1"
                     /translation="MAVKDKGSRQAHFPKIEAKYGQPMRYWFAVMKDISDLKYPQQIS
                     HLREEHGFSQAHANALVMYSRGSKSSKRFDDLEGYLKGQDEVKRKTIKKIFRVIQKKY
                     PKLELVIAWNQPMLKLGNEYLFGASILKNHILIAPWNQKVLLAMKQDLEGYKVNKKTV
                     QVPVDWKVDEKLLLKMIKLNLQFLKK"
     gene            complement(1611..1682)
                     /locus_tag="PLW53_01750"
     tRNA            complement(1611..1682)
                     /locus_tag="PLW53_01750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1648..1650),aa:Val,seq:tac)
     gene            complement(1703..2161)
                     /locus_tag="PLW53_01755"
     CDS             complement(1703..2161)
                     /locus_tag="PLW53_01755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD36184.1"
                     /translation="MAISIGDLAPDFELVNQFGEKVSLSSFRGTKNVVLVFFPFAFTG
                     TCTGELCALRDDLSAFQNENIELLAISCDAMFTQKVFAEKEGYNFPLLSDFWPHGAVA
                     KKYGVFNEDRGLALRGTFIIDKAGIVRWSVINSPAEARKIEDYRSALASL"
     gene            complement(2161..2583)
                     /locus_tag="PLW53_01760"
     CDS             complement(2161..2583)
                     /locus_tag="PLW53_01760"
                     /inference="COORDINATES: protein motif:HMM:NF022698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HRD36185.1"
                     /translation="MSTPPVGPGQLASRMGIQSGFLVLERGFGSDCDEAIRSEISTAT
                     GSEFLDENSQEVVDAVITWWREDDGDLIDELVDCLTYLSESGPIWLLTPKVSRPGHVE
                     PSDIQDAAQTAGLSLTSTIPASKDWTATRLVARKSGRK"
     gene            2756..5473
                     /gene="aceE"
                     /locus_tag="PLW53_01765"
     CDS             2756..5473
                     /gene="aceE"
                     /locus_tag="PLW53_01765"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRD36186.1"
                     /translation="MNNLQDLHVDQLIDANPDETAEWHQSLDSLIKHAGPARARYLML
                     SLIKRAHEANIQLPNLRLTDYMNTIPPESEPQFPGDEFLERRIRAYIRWNAAIMVHRA
                     QRPGVGVGGHISTFASSAALYEVGFNHFFKGKDHPGGGDQIFFQGHASPGMYARAFLE
                     GRLSEHQLDGFRQELSHSGGGLSSYPHPRLMPEFWEFPTVSMGLGPINSIYQARFNRY
                     LANRGIKDTSGQHVWAFLGDGEMDEPESVSALTLAARENLDNLTFVVNCNLQRLDGPV
                     RGNGKIIQELEALFTGAGWNVIKVVWGREWDPLFAMDKEGALVELMNKTPDGDFQTYK
                     AESGAFVRENFFNRDPRTAAMVANWSDDDIWNLKRGGHDYQKLYAAYQSAMNHKGQPT
                     VILAKTIKGWTLGSHFEGRNATHQMKKLKLEDLKSLRDRLYLEIDDDKLDEKLPPYFH
                     PGKESPEFQYMMEKRAELGGSVPRRRSKSKPLPQPQDSDFAVMTRGSGAQEIATTMAF
                     VRLLKDLAKVEGLGHRIVPIIPDEARTFGMDSLFPTMKIYSPHGQQYLAVDRELMLSY
                     KESTSGVILHEGINEAGSTASFTAVGTSYSTHDEPMIPVYIFYSMFGFQRTGDAFWAA
                     ADQMARGFVMGATAGRTTLNGEGLQHEDGHSQLLASTNPAVVAYDPAFAFELGHIVKD
                     GLRRMYGENSENIFYYLTVYNEPYVQPAEPENLDVEGLLKGIYLYSKAKKQRRAKRQV
                     NLLASGVALNWALKAQNLLASDWKISANVFSVTSWNELRRDGLEVDRHNLLNPLDKKS
                     AYLTTKLKNYKGSVLAVSDYMRTVQDQIAPWVEQNFASLGTDGFGLSDTRGALRRHFK
                     VDAESIVVAALSQLANQGKISPKVVKKAIEKYQLNNVTAADPGNTEGSG"
     gene            5470..7215
                     /locus_tag="PLW53_01770"
     CDS             5470..7215
                     /locus_tag="PLW53_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HRD36187.1"
                     /translation="MISRFGIIDISPATFFGGEFIGAKAVVNEAVAISATIIREGHES
                     FDAFAVLVDESGKIASREKMVEIWPNSQRFEAFLRPKALGNWSFYIEVSAENPGEFAK
                     TLMTKSESYPIKVERERALVGNWYEFFPRSEGAKKNSDGSISSGNFRSAAERIEDVAK
                     MGFDVLYLPPIHPIGYSHRKGRNNSLIAGPLDPGVPWAIGNESGGHDAINPELGSLDD
                     FKYFLKIAKKNNVEVALDLALQCSPDHPWVKTNPEWFTKRADGSIAYAENPPKKYQDI
                     YPLNFDNDYPGLFAEIYRVVTQWIELGINIFRVDNPHTKPINFWADFIAKINQKYPQV
                     VFLAEAFTTPAMMHALGKAGFQQSYTYFTWRVTKPELEEYAKEVAYQTSAFFRPNFWV
                     NTPDILPFHLQSGNPAAFALRAVLASTLSPSWGMYAGYELYEHIPIGEGKEEYRDSEK
                     YEIKVRDWQAAAKKSLTLAPFITKLNEIKNQNVALQRLRNITFHQSDSDQVIAYSKKE
                     GDNLILVVVNLDPFKAIETMVHWNLSALGLSDKGFEVTDLIDQAKYSWSSDTFIRLDP
                     TRPMGRVAHIARVKK"
     gene            7231..9171
                     /gene="glgB"
                     /locus_tag="PLW53_01775"
     CDS             7231..9171
                     /gene="glgB"
                     /locus_tag="PLW53_01775"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRD36188.1"
                     /translation="MRRLFKNLFSKRSAFQSPPAGYLNPHSTLGELDMYLITEGRHES
                     LWEVLGAHVKRDEHGELIGTAFSLWAPNAKRVSLICDANFWDKEINPMIPLGSNGLWE
                     VFIKDVGPGLKYKFAIQGRDSRWVDHADPLARQSERPPHTASIVNESNYQWSDSKWMD
                     SRGLFQPWKSPVSIYEVHLGSWRAGLSYRQLAQELGQYLIEYKFTHVEFMPVSEYPYD
                     PSWGYQVTSYFAPTSRFGSPDDFRYLIDHLHSLGIGVILDWVPAHFPKDEWALARFDG
                     TCLYEHEDPRLGEHPDWGTLIFNYSRNEVRNFLVASALYWLESFHIDGLRVDAVASML
                     YLDYSRKEGEWIANEFGGRENLAAVKFLQEATATAYKRFPGIMMIAEESTAWTGVTKP
                     TSENGLGFGFKWNMGWMHDSLQYLSHDPIHRLYHHNEITFSILYAFSENFLLPLSHDE
                     VVHGKAALVNKFPGDRWQKVATLRALYGYMWSHPGKKLLFMGSEFAQNDEWSHDKSLD
                     WHLTQYGEHKGVQDLVRDLNALYKESPALWQRDSSSDGFTWLVGDDGAGNTLAYQRLA
                     DDGSQIISITNFSPVPHEQYRLPIPKSGNWIEALNTDDVKYGGSGVINNQIKVDQIQH
                     RGFDYSAVIRLAPLATIWLKLN"
     gene            complement(9168..10331)
                     /locus_tag="PLW53_01780"
     CDS             complement(9168..10331)
                     /locus_tag="PLW53_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36189.1"
                     /translation="MQAFFFLFGLGIMCLAPRLPDIKANLDVSTSYFGFLMSTGSIGA
                     LSAQLVMGHIVHRLGVYRVLIYSSTLTYLTLGILIELKSAPIYLVVNVLCGFAWASYH
                     IAINAQALHRQHESGKQIIPMLHGLWSAGAVTTAFIALLISSFVSLNWHILTLISLVY
                     LLTLRSIKASKAYLLQGHEVTEDDEVITFKKMITSFQIDWVVSLGFLCALLLEVSIGD
                     WATIMARQEFGVSKSVAVTPYFLFMGAMIIGRLTYSRIKGNRSDKELVQPFVVMGGTV
                     FLTCLALSMQIKESNLVLGYTIFCLGFLLTGFGISFVGPLFFGYASARSNKPGGVAVA
                     ELGAMNQVLTFICRGVISLVAGAATLPIAMAIPGLMLILVAFFVSAASQPKRG"
     gene            complement(10364..11236)
                     /locus_tag="PLW53_01785"
     CDS             complement(10364..11236)
                     /locus_tag="PLW53_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD36190.1"
                     /translation="MSMPPAGFGRAFDLSSLTKPKVEVGATSSDEATVENFMADYVGR
                     SKEKPVVLLAYSPRSAATVELRQLMASIAKEDNDSWIFGAINADTQVQLVQALKITAV
                     PFAIAFINEQPVALFDRIYPREQIVMVITKLFELAKEQGLNVDVPEVKEIPMEPEEAA
                     ALSALEKGDYSGAAMAYRNWLMRKPDESVAKIGLAQCELMIRISALNPSLTIKDADSD
                     PTSIEKAVMAADVEIAQGLQKNAFARLINFVKNSSGDEKKRAKEHLLLLFQLVDPADP
                     DLIRSRNELASALF"
     gene            complement(11249..12484)
                     /locus_tag="PLW53_01790"
     CDS             complement(11249..12484)
                     /locus_tag="PLW53_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HRD36191.1"
                     /translation="MAATDKQLHIKGLGEEISALYNLPWHTPLEQWPEDETLTAQRGI
                     SRHIVRLVRSTPDPASEIYAVKETVEEFAVREYEALRELSLRGAPAVAQVAIVTNRYS
                     QNGEELPCAIVTRFLPYSLPYRVILSGQITQHEITNMANALAYLLVRLHLLGFWWGDC
                     SLSNTLFRRDAEGFAAYLVDAETGEFQKKLSDGQREHDLELARFNVAAELEDLKIAGV
                     LFPAMDPIRASESVITRYRKIWKSLSEPQILPAGDRHAVERAMRSLQDIGFAVEEVDI
                     QLAGDKSTVTFIPKLVAPNYHSQRLIELMGLETEELQAKRILASFDRFRSREIEKTPK
                     KEEAAKRWLDEVFYKVINAVPTAMRGRVEDAQLFHEVLEHRWYLGEKAGRDLGLEFAT
                     NDYISKVLPERMDSGAPTL"
     gene            12517..13644
                     /locus_tag="PLW53_01795"
     CDS             12517..13644
                     /locus_tag="PLW53_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRD36192.1"
                     /translation="MAKIRGVLKKVGARPSKVQEVQDFGKKGAPLNTQHPFYFGFLAA
                     SGALVAITLLRALQSASQVFVLIIISLFLAMGLNPTVDALQSRKLKRSSAVAVVVVVA
                     LAIIALFALIVIPPVATQANDLIESAPQLLDSLRSNATLNQLNVDYGFIDSLEKKFEE
                     WISDGKILTGAFGGVLGVGRTVLSGAFSALTVLVLTLYFLTSLPSVTKIFYRLAPASR
                     RARVSSIGDAIISRVGSFVGSQVLIAGLAALFVFALALGLALPYAAALSMVILFVALI
                     PLIGHFLGASIVILVALTQSPSKALLALILYTAYVQIENFIITPRIMKRSLAIPGLVT
                     IVAALLGTSLLGLVGGILAVPIAAAILLIMDEVVFPKTDNS"
     gene            complement(13646..14359)
                     /locus_tag="PLW53_01800"
     CDS             complement(13646..14359)
                     /locus_tag="PLW53_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD36193.1"
                     /translation="MAELIRPSTVLPAERSAITFLTSDGLKLIGEIARPIQDNQGIGL
                     LCLHPLPTHGGMMDSHIYKKAANRLPHLAGINVIRFNTRGTKSDQGISEGQFGEGITE
                     REDVIAAINYSFDQLGIKKLWVSGWSFGSDLALKYARDKRVMGLILLSPPLRFSTDED
                     LAFWGQDGRGITALIPEFDDYLKPEQAKQRFVPIPQAELIAIKDGKHLWVGEPFVHRV
                     LSEIVKVISPERLPIPLEI"
     gene            14382..15851
                     /locus_tag="PLW53_01805"
     CDS             14382..15851
                     /locus_tag="PLW53_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD36194.1"
                     /translation="MKTFESLNPATGDVIGVFSQTSAKEVDESVAKASIASEHWASLS
                     FRKRGIILRDWASLLTREIESSADLIHRETGKPLSDARLEVSIAIEHIGWAAKYAPKV
                     LSDQSRPSGLLMFNMAAKVQRSPFGVIGIIGPWNYPFFTPVGSIAYALAAGNTVVFKP
                     SEFTPAVGAWLGESWKRIAPFADIFTVITGGAETGAALTKSKVGKISFTGSTKTGKLV
                     AAACALNMTPVLLECGGKDAVLIDRDADLKKAAEVTLWHAMANAGQSCIAAERVYVHR
                     DVAERFSTLIVEQATKIKAGYEDGANYGPATMPSQLKVIKAHIDDAEKRGGRFLFGDK
                     SAVKGNFVSPVILADVPEDSKAISDETFGPTLIINTVRNMDEAVDLANASNYGLGAAV
                     WSKKNGKRIASLLKCGMVSINSAFSNTAVSSLPFGGIKDSGYGRTHGAEGLLEFTYPR
                     AIIKPRFRIPINFTTFDRSQRADRIVMLLTKILHKRRRR"
     gene            complement(15848..16165)
                     /locus_tag="PLW53_01810"
     CDS             complement(15848..16165)
                     /locus_tag="PLW53_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36195.1"
                     /translation="MSPRRNRPKQRGRKIKSDEESDSPSHQSIEEHSEGIYMVRKITG
                     SSSNKPYRCPGCDQLIPMATPHIVAWIEHDVESRRHWHSACWSKKDKRKPKIERSRNA
                     PRF"
     gene            16203..16775
                     /locus_tag="PLW53_01815"
     CDS             16203..16775
                     /locus_tag="PLW53_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36196.1"
                     /translation="MKSKLVKILITCLAATLLSTATGQADQVKVVAKKTAKPIPSPAA
                     KWPPEGFKGKEGVFAKVPSSKEVIGLLSAKKSLQPLVKTCEEFACGAVFVASETGCKW
                     WEVNSSVFSLKASDLSKEKIGTLTTYHASSKEKAQATIFLISGAEVTPGTSIGSIKVI
                     CHRDSSNKPKPGNIYSPIVAPTPTASPSNS"
     gene            16785..17105
                     /locus_tag="PLW53_01820"
     CDS             16785..17105
                     /locus_tag="PLW53_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36197.1"
                     /translation="MLGGINNTLFFTSFAGFAVMGLFIILLKWAFSRGSSLVEKPKKI
                     GDEDEYGLLKVVASPSNFVEGEMLRLKLDEHGIKATLTQTKSGPKILVFEKEISVAQA
                     ILRS"
     gene            complement(17102..17674)
                     /locus_tag="PLW53_01825"
     CDS             complement(17102..17674)
                     /locus_tag="PLW53_01825"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRD36198.1"
                     /translation="MVNLTRIYTKTGDDGTTSLGDMSRTSKNDPRLEAYATVDEANSY
                     IGVVLASANLDKEISKLLVRIQNDLFDVGADLCTPVVDNPEHEPLRVLQSQVDYIEAQ
                     IDHYNASLAPLKSFVLPSGTPASAHLHVARTVVRRAERRTWQAIHSFGEGVNKLTAKY
                     LNRLSDLLFVLARYENRESGDQLWVPGKNR"
     gene            17757..19052
                     /gene="murA"
                     /locus_tag="PLW53_01830"
     CDS             17757..19052
                     /gene="murA"
                     /locus_tag="PLW53_01830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRD36199.1"
                     /translation="MAKLYPIAGQINPDRFRVVGGARLAGDVYISGAKNSVLKLMAVS
                     LLAPGSSTIRNVPDIADVSMMAELLETLGCKISRDFESKSITIDVPEAPGHRAEYESV
                     RKLRASINVLGPLVARLGKAEVALPGGDAIGSRGLDFHTKGLVALGAEAHNEHGYIMA
                     SAAKGLTGTQIELEFPSVGATENIMSAAVLASGVTTLENAAREPDIVDIGNFLIAMGA
                     DIQGLGTSTITITGVKSLKGATHTAISDRIVTGTWAFAATMTLGDISIHGARPEHLEL
                     PLEKLVSAGAVITSISDGIRVKMDRRPTAIDVATLPYPGFPTDLQPFVITLNAIAEGD
                     SLVTENVFEGRFMFVNELLRLGAKIRVDGHHASITGIKELSSAPVVASDIRAGAGLVL
                     AGLVSEGITFIEGGHHIDRGYPDFAQQLQGLGADVTRVD"
     gene            complement(19057..19803)
                     /locus_tag="PLW53_01835"
     CDS             complement(19057..19803)
                     /locus_tag="PLW53_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36200.1"
                     /translation="MRRSSFFLIALALTLSAGPALAHYPVNLKASHNTVSKSPILLDG
                     TISFAVYADFAKAKDQRSVRFALKEGDELNVEYLIVDAAPTNKLKSSQLPGVTITTPS
                     GKKIVMKINERSPFYEPYGKKNYFFLSRISQSAEAGIYSITATAKAKSSAVLAIGRTE
                     TRGEYLEVGSSARTCPITLKNEDLISEGRANQLISMSEVEAEVCAAANSWVYRIGEKD
                     GEEFMLTKDYRTNRVTVSIESGFITKVSVG"
     gene            complement(19790..20053)
                     /locus_tag="PLW53_01840"
     CDS             complement(19790..20053)
                     /locus_tag="PLW53_01840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRD36201.1"
                     /translation="MSDKTLTVEFVTPEKRVFSGKAISLSARTLEGDLGILVDHAPLL
                     GVLVDGKVSIEGVDGSVQEFEVSGGFISVANNRISVLGESATQ"
     gene            complement(20057..21478)
                     /gene="atpD"
                     /locus_tag="PLW53_01845"
     CDS             complement(20057..21478)
                     /gene="atpD"
                     /locus_tag="PLW53_01845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRD36202.1"
                     /translation="MTTKTATGRVARVIGPVVDIEFPADSMPAIYNALNVEVTLGGEM
                     RKLTLEVAQHIGDNLVRAISMQPTDGMVRGVEVSDTGAAISVPVGDVTKGHVFNALGE
                     SLDVPTSSLDIKERWPIHRTAPAFDQLESKTEMFATGIKVIDLLTPYVKGGKIGLFGG
                     AGVGKTVLIQEMIYRVAENFGGVSVFAGVGERTREGNDLFLEMTETGVINKTALVFGQ
                     MDEPPGTRLRVALSALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAV
                     GYQPTLADEMGVLQERITSTRGHSITSMQAIYVPADDITDPAPHTTFTHLDATTVLSR
                     PISELGIYPAVDPLDSTSRILDPRYIGENHFRVANRVKQILQRYKDLQDIIAILGIDE
                     LSEEDRILVGRARRIQRFLSQNTFVAKVFTGLDGSFVPLTETIAAFDALANGDYDHIP
                     EQAFFMCGGLEDVERKAAELAKK"
     gene            complement(21478..22392)
                     /locus_tag="PLW53_01850"
     CDS             complement(21478..22392)
                     /locus_tag="PLW53_01850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRD36203.1"
                     /translation="MGAQLRIYRRRIRSVKATKKITRAMELISASRIVKAQQRVAAST
                     PYANELTRAVSAVATFSNTDHPLTTESTNPKRAAVLIITADRGMAGAYSSSAIKEADG
                     LVITLKERGLEVNTYLVGRKAVNYFKFRNRPIKNSWTGFSDNPTYAHAKTIADELIPA
                     FIADAATDINGVDELHIVYTEFKSMIAQIAMNKRMLPLEVVEAEGPAPAGLLPMYEFE
                     PNAAEVLNALLPRYIESRVYNALLQSAASEHAARRRAMKSATDNADELIKSLTRLANA
                     ARQAEITQEISEIVGGADALASASAGSE"
     gene            complement(22397..24022)
                     /gene="atpA"
                     /locus_tag="PLW53_01855"
     CDS             complement(22397..24022)
                     /gene="atpA"
                     /locus_tag="PLW53_01855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRD36204.1"
                     /translation="MAELTIRPEEIRDALANFVKSYDPGSASRDEIGTVVVAGDGIAR
                     VEGLPSTMTNELLKFENGTLGLALNLDVREIGVVILGEFTGIEEGTTVRRTGEILSVP
                     VGDGFLGRVVDALGRPIDGKGEIKAETTRALELQAPSVVQRQPVKEPLATGIKAIDAM
                     TAIGRGQRQLIIGDRQTGKTAVAIDTIINQRDNWRSGDKKKQVKCIYVAIGQKGSTIA
                     SVKGALEEAGAMEYTTIVAAPASDPAGFKYLAPYTGSAIGQHWMYGGGHVLIVFDDLS
                     KQAEAYRSVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSMTGLPIIETK
                     GNDVSAFIPTNVISITDGQCFLETDLFNSGVRPAINVGISVSRVGGSAQTKAMKKIAG
                     RLRLDLAQFRELEAFAAFGSDLDAASKAQLERGARLVELLKQQQYTPFSLERMIVSIW
                     AGTTGQLDSIPVADIRRFESEFLDYVGREQKAIFDVIAESKELTDDTVAKLTAAINTF
                     KEQFASSSASAVNEAPAQAEGSDGTEQITKYKK"
     gene            complement(24037..24876)
                     /locus_tag="PLW53_01860"
     CDS             complement(24037..24876)
                     /locus_tag="PLW53_01860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRD36205.1"
                     /translation="MSRAMAALGGASRQSLIAARKSLDELLKGLSAAEASALSAHLFA
                     VVATLDSSTPLRRALTDNSRDANSKAELAKELFAKSTSDLSIKLLISLTALRWSSPSN
                     LGDVIEQLAVEAEASVANQNNELDRLEEEIFQFSRIVASDLELRQILNSSKYSSEGKR
                     VLVAKLLASKVCPSTSRLLASLVSGMRGRSIEKTIAFYASAAAARKMRVIAHVKSAVE
                     LSQAQKDKLASSLSGKIGQPVRLNVELDPKVLGGLSIRFADELIDATIVNRLADAGRA
                     LAV"
     gene            complement(24879..25406)
                     /locus_tag="PLW53_01865"
     CDS             complement(24879..25406)
                     /locus_tag="PLW53_01865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRD36206.1"
                     /translation="MRSIELAAEELNPLIPHTAEIIVGFIAFSLLYMVLRRAVVPRFE
                     KAFAERTDAIQGGMERAEKAQLEAEAALKQYKAQLQDARGEAASIREEARAQGAAIIE
                     EMRVKAQEEATRIAASAQAAIESERQAALTSLRNEVGTLATQLASKIVGEALDDQVRQ
                     SRIVDRFLEDLEKSK"
     gene            complement(25423..25626)
                     /gene="atpE"
                     /locus_tag="PLW53_01870"
     CDS             complement(25423..25626)
                     /gene="atpE"
                     /locus_tag="PLW53_01870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRD36207.1"
                     /translation="MEGTLNLVGFGLAAIGPGIATGLIFAAYINGVARQPEARSVLQP
                     IAFLGFALAEALALFGLVLAFVL"
     gene            complement(25695..26483)
                     /gene="atpB"
                     /locus_tag="PLW53_01875"
     CDS             complement(25695..26483)
                     /gene="atpB"
                     /locus_tag="PLW53_01875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRD36208.1"
                     /translation="MLSYAAEVGKDEGFNPPDAGDFNLPPFIEGNEFATKPILLVFLS
                     VILISIFFILASRKAALVPSKLQFAGESVYSFVRNDLGREVIGHEFMRFVPYLFTLFT
                     FILVNNVFGIVPMLQFPTMSRISFPYVLAAFTFFIFHYVGIRKQGFFKYMKEIMFLPG
                     IPKPAYILITPLELATYFLVRPLTLSLRLFANMFAGHLLLLVFILGGEHLLQGVIGLK
                     LISPFAFAIGIALTFFEFMVQCLQAYIFTLLTALYIAGALADEH"
     gene            complement(26631..26837)
                     /locus_tag="PLW53_01880"
     CDS             complement(26631..26837)
                     /locus_tag="PLW53_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRD36209.1"
                     /translation="MSSNEPKRPSEDNAFWTIFGYMLSGLLIWGGIGWGLDQWLNTGF
                     FLLAGLLLGMGSALYLVWLRFGRK"
     gene            complement(26840..27265)
                     /locus_tag="PLW53_01885"
     CDS             complement(26840..27265)
                     /locus_tag="PLW53_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36210.1"
                     /translation="MDIASSNKQMWRGALSASLVATVIAIVFFTIYLGAPGFFGSALA
                     SFVVLIFFSVSILVGILSRNSDPISVMALALMSYFTKLLLIALFLIAVTRLSEPDSLD
                     RSAFGISALAISAAWLSGEVRAFFKLRLELPQPKSQSKE"
     gene            complement(27266..28345)
                     /locus_tag="PLW53_01890"
     CDS             complement(27266..28345)
                     /locus_tag="PLW53_01890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRD36211.1"
                     /translation="MREYLLTLLIAAVLTYMCTPLIRSLALRSKAVASVRERDIHTLE
                     TPRWGGVAMWLAMGATLVMVGSLNLVGKAYSQELLGIFLASTFVLLIGALDDRYELDA
                     ITKLAGQGLAAAILLIFGIQILWLPIDGIIVLPTNIGQLLTVVVVVVIINAVNFIDGL
                     DGLATGIVGISAAAFFGFSYLLAVENGFSRAGAPSLVTAIVIGCCLGFLPHNTHPAKI
                     FMGDSGSMFLGLLLAASAITLMGQIDANALFAENIGPAALPLLLPFAVLAIPLLDLAL
                     AVLRRVKSGRSPFAPDQEHLHHKLMAWGNSQQRSAVILYLVTAMLALPAMLSAFIPLW
                     AALLVGALLLLSAVITTKREKKVKA"
     gene            complement(28345..29610)
                     /gene="glyA"
                     /locus_tag="PLW53_01895"
     CDS             complement(28345..29610)
                     /gene="glyA"
                     /locus_tag="PLW53_01895"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRD36212.1"
                     /translation="MSETFFGPDFQALQEQDSEIAAILISELDRQRQNLQLIASENFT
                     SPAVLAALGSTLSNKYAEGYPGKRYYGGCEEVDKAEVIGIARAKELFGAEHANLQPHS
                     GASANVAVYQAFTKPGDTVLAMSLPHGGHLTHGSKVNFSGKWFNVESYGVRQDNELID
                     YDELRDIALRVKPKMICSGATAYPSLIDFKTVRSICDEVGAIMWVDAAHFIGLVAGKA
                     IPSPVEYADVVSFTTHKVLRGPRGGMILSKAAHASAIDKAIFPGMQGGPIMSAVAAKA
                     IALKECATTQYQAYAKQVIVNAKALAKSLEDEGMRAVSGGTETHLALIDIRSTGVNGK
                     VADERCGRAGISLNKNAIPYDPESPAVTSGIRVGTPAVTTQGMGTEQMPVIASLIARA
                     IKDGEDEAKIAQIRKEVNALTAKFPVYPA"
     gene            complement(29621..30274)
                     /locus_tag="PLW53_01900"
     CDS             complement(29621..30274)
                     /locus_tag="PLW53_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRD36213.1"
                     /translation="MAERYSLTDGLIDSNELIERAVASLRDGKLIIAPLEHGYVYLAD
                     AFNHGAVKKIHKLRQSPPATAAQVIVGDVKTVKGISLQFGETITALAEKFWPGLLTIN
                     LTPAQGLVWDLGDARALNEISVRVPNAEFVRSVALASGPLAVGAACLSGRPVPRKSTF
                     FPALDSDYAALFDLGELPEGPSSTVIAIEQEGARLVRAGAISLADLRSVSANISVPA"
     gene            complement(30274..31143)
                     /gene="prmC"
                     /locus_tag="PLW53_01905"
     CDS             complement(30274..31143)
                     /gene="prmC"
                     /locus_tag="PLW53_01905"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRD36214.1"
                     /translation="MSIKQVLKDKKTQFASAGISEVDAELILAFVLGVERMELHARDF
                     DLELEQVELLDDLITRRISGKPTQYLIGHAPFRYLTFQVGQGVLIPRPESEALVEAAL
                     IEIEKIHSMQGQSSKTPVSVVDLGSGSGALAISVAHEAEQKNWPVTVVAVEKSDEAIK
                     WLKRNIAACDVSVRVISGDVLEVLEGVKCDIVIANPPYVPDGDKLPELVIENEPREAL
                     FGGGADGLEIPRRFIKAASILLKPGGLLIMEHHESQSLLLEAELAKGYSEINLNCDLN
                     NRPRWISARREAE"
     gene            complement(31149..32243)
                     /gene="prfA"
                     /locus_tag="PLW53_01910"
     CDS             complement(31149..32243)
                     /gene="prfA"
                     /locus_tag="PLW53_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRD36215.1"
                     /translation="MAEKNANRFSTMPEILKEYEQLEKDMADPSIHADQGKARQLGKR
                     YAQLGPVIAGYRAFKKSEEDLTAARELAEVEPDFAAEIPALEKAYEEYASAMEELLLP
                     RDPNDDRDVILEIKAGAGGDESALFAGDLLRMYLRYAEKQGWKTEIIDVTESDLGGYK
                     DISVAVKARGGAAAGSAPFSKLKFEGGVHRVQRVPETESQGRIHTSAAGVLVLPEAEE
                     IDVQINMNELRIDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVSCQNEKSQLQNKESA
                     LRILRARMLAAAQEAADAAASATRKSQVRTVDRSERIRTYNFPENRISDHRVNFKANN
                     LDAVLNGELDEVIGALLEADKAEKLSAAEA"
     gene            complement(32341..32598)
                     /locus_tag="PLW53_01915"
     CDS             complement(32341..32598)
                     /locus_tag="PLW53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRD36216.1"
                     /translation="MRKDIHPEYGYVIFRDTSADFAILTRSTMKSDQKMVWSDGKEYP
                     LVTVEISSASHPFYTGKQRILDTGGRVAKFEERAKKQQTKK"
     gene            complement(32706..34313)
                     /gene="rho"
                     /locus_tag="PLW53_01920"
     CDS             complement(32706..34313)
                     /gene="rho"
                     /locus_tag="PLW53_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRD36217.1"
                     /translation="MAETSTRARRESGDLSALLLPELRKIATNLGIEGAAELKKPDLV
                     TAITDLQAANREAAKAEREAKRAARNQKRRDNNSNETASSDSDDDQDEDEMPSDNSSN
                     NSNNSNNNNSRDNSRGDREWRRDRHRDRGRDRFRDRDRSNRGEREIVLSEDDVLLPVG
                     GLLDILDNYAFLRTAGYLPGPNDVYVSLQQVRRYGLRKGDVVTGQVRQAREGEKREKF
                     NALVRLDTINGMEPDSSKERVDFSKLVPLYPQDRLRLETLPNILTTRVIDLIAPIGKG
                     QRGLIVSPPKAGKTMVLQAIANAITTNNPECHLMVVLVDERPEEVTDMQRSVKGEVIA
                     STFDRPADDHTTVAELAIERAKRLVELGHDVVVLLDSITRLGRAYNIAAPASGRILSG
                     GVDSAALYPPKKFFGAARNIEDGGSLTILATALVETGSKMDEVIFEEFKGTGNMELKL
                     DRRFADKRIFPAVDIDASGTRKEEILMGPEELNIVWKLRRVLHALDSQQALELLLEKM
                     KGTKSNSEFLMQVQKTTVGPDQSPKGE"
     gene            complement(34492..35439)
                     /gene="thrB"
                     /locus_tag="PLW53_01925"
     CDS             complement(34492..35439)
                     /gene="thrB"
                     /locus_tag="PLW53_01925"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRD36218.1"
                     /translation="MSRGFKDRLTYKATPMQASVPASSANLGPGFDCLALALELRDTY
                     VAQILDDEVFDVDVTGQGADDVKKDGKHLVIKAMMAGFEFMGGKPRGIALRCLNEIPH
                     GRGLGSSAAAIVGGLSLSRSLVLSGSNLMSDDDLIALATDIEGHPDNVAAATFGGATI
                     SWMEDRAKVVTGCASGFSVDPNIRALLFVPDTQLSTGKARKMLPEQISHKDASINSGR
                     SALLVHALSSRPELLFAATQDLLHQSYRREAMPKSMDLVNKFRKAGVAAMISGAGPSV
                     LVLHTATKAEHDDLIRSGGDYFKAMDLEISPTGVRIAAV"
     gene            complement(35442..36521)
                     /gene="thrC"
                     /locus_tag="PLW53_01930"
     CDS             complement(35442..36521)
                     /gene="thrC"
                     /locus_tag="PLW53_01930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRD36219.1"
                     /translation="MSLFKKSGAHQWRGVITEYKKRLPVSSKTPIVTLNEGGTPLIYA
                     CVLSEMLGNDVWLKFEGANPTGSFKDRGMTMAISKAAEDGAKAVICASTGNTSASAAA
                     YATKAGMVPVVLVPQGKIAMGKLAQAIAHGAKLLQVKGNFDDCLTLAKQLSEQYPVSL
                     VNSVNPYRIEGQKTASFEVIDALGFAPDIHVLPVGNAGNITAYWKGYKEYYADRISKS
                     LPAMWGFQAKGAAPIVRGKIVKNPETLATAIRIGNPASWKQAEQARDESKGLIDMVSD
                     SEILSAYRLVAAKEGVFVEPSSAAGLAGLIKYKAAKKLPKGKKIVITVTGNGLKDIQW
                     ILNGADKPITVPVDVKKAAKVIGLS"
     gene            complement(36521..37816)
                     /locus_tag="PLW53_01935"
     CDS             complement(36521..37816)
                     /locus_tag="PLW53_01935"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRD36220.1"
                     /translation="MISAQQSFNVAMLGCGVVGSEVARLLLADDGDFAARSGAKLNLT
                     RIAVRDSKAGRERIPKELLTQDAESVVSDPSVDIVIEVMGGIEPARTLILKAISNGKS
                     IITANKALLAQHGAELFSAADKAGVDLYYEASVGGAIPILRPMRESIVGDHVNRVMGI
                     VNGTTNYILTKMDEEGSSFEEVLKEAQYLGFAEADPTADIAGHDAAAKAAILAGLAFH
                     SRITASDVYCEGISQISARDVAVARDMGCLIKLLAIAELTKENEISVRVHPTLISRRH
                     PLAAVRLAFNAVFIEAQSAGELMFYGRGAGGEPTASAILGDLIAIARNKIRGGQGHGE
                     SDYADLAIAPIDNVKSQYLIRLDVADKPGVLANVAQLFAANSVSIETVRQTGRGDSAE
                     LIVATHYAPESALKKTVKSLADSDVVKSVESVLRIEGSK"
     gene            complement(37813..39186)
                     /gene="lysA"
                     /locus_tag="PLW53_01940"
     CDS             complement(37813..39186)
                     /gene="lysA"
                     /locus_tag="PLW53_01940"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRD36221.1"
                     /translation="MSQDLNPVIWPLSARRVNGGITIGNISVRDLADEFETPAFILDE
                     SDFKARASLWAKALQEVFGANAGTAYYAAKSFISTQVARWIEQAGLGLDVCTDGELAV
                     AIAANFPAKKIELHGNNKSESEIAAAIDYGVGVIVIDSLQEIDRVARIAHEKSVTQGV
                     LLRLNPGVDADTHEAISTGHEDVKFGFSIASGAAWDAILSVKKHDSLELRGLHAHIGS
                     QIFTPDSFELSASRLIAVLAKYRDEFGAELAELDLGGGYAIAYIEGDKPSSANEVLSV
                     IARRVKTECEKALLKIPRISIEPGRVIVGPAMVTIYKVGTTKEVTLADGSTRWYVSVD
                     GGMSDNIRPALYEAQYSITLANRDSSAPLRKSRVVGKHCETGDIIIRDVQLPSDIAPG
                     DLLVVPATGAYGRSMASNYNHMTRPPVICVLNGKARSIIRRETHRDLLALEIEEEPRQ
                     IGKGGSK"
     gene            complement(39189..40898)
                     /gene="argS"
                     /locus_tag="PLW53_01945"
     CDS             complement(39189..40898)
                     /gene="argS"
                     /locus_tag="PLW53_01945"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRD36222.1"
                     /translation="MSASNSPNSPELAPHELLSAAILESVKAAIAKGELPAELSSNAA
                     LDEITLERPKNRDHGDYATSIALALAKQAGKPPRQIAEIITTSLKSNEIIERIEIAGP
                     GFINITLVKASQGAVVNTIYAQGDKFGEVKILSGVKINLEFISANPTGPLHLGHTRWA
                     AVGDALARVLIAGGAKVAREFYINDRGNQMDLFGASLAASALGQDKPEDGYQGEYIDE
                     LAQEILIKNPEYKKLSGPGAIEAFREAGYELQLAQQKRVLDNFGTHFDIWFSERSLHS
                     GDGIDKALATLKSQGHTYENEGAIWLRTTDFGDDKDRVLVKSGGELTYFSSDTAYYLD
                     KRRRGFDICIYMLGADHHGYVGRLKATAACAGDDPEYNIHVLIGQLVKIIEGGQEIKL
                     SKRAGTIITLEELVEKVGVDAARYTLIRYPVDTPMVLDIDLLKKRTNDNPVFYVQYAH
                     ARICAVLRNAKDLSIPYGINHYQADLLTHDRERELLGSLAEFPRVVASAAELRQPHRI
                     ARYIEELATLYHGFYNDCRVLPLGDESPSAIHSSRASLCDATRQVISNALNLLGVSAP
                     EKM"
     gene            41119..42189
                     /locus_tag="PLW53_01950"
     CDS             41119..42189
                     /locus_tag="PLW53_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD36223.1"
                     /translation="MSKRRLAISALVASFALILTGCSSSDEGSSDGFVGVILPDAASS
                     ARWETADRGFLQAAFDAAGVKVDIQNANGDAAAFQTIADGMLSAGAKVLILVNLDSES
                     AKAVQDKAAEQGVPTIDYDRLTLNGSASYYVSFDNEAVGRLQGEGIKACLDGAGKTKA
                     RLVYLNGSPTDNNATLFKAGYDSVLRPLIDAGDYTLVDDQAVPDWDNVKGGTIFEQQL
                     SKAGGKLDAVVSANEGLGLAAVAVLKKNGLNGKVCVSGQDATVDGLRAILTGDLSNTV
                     YKAIKAEAEAAAALAIALLNGEEATTATGSVNNGVTDVPSVLLVPVSITKANVKDVIA
                     DGFQTREAVCEGIEDLCTANGI"
     gene            42293..43030
                     /locus_tag="PLW53_01955"
     CDS             42293..43030
                     /locus_tag="PLW53_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36224.1"
                     /translation="MASPILSLKRVNKSFGPVHVLKDVDFSAYAGKVTALVGDNGAGK
                     STLIKCIAGIYTPETGEFIFEGNPVTISGPRDATALGIEIVYQDLALCDNLDIVHNMF
                     LGREEISGITLNESSMESLAKKTLDGLSVRTVKSIRQKVASLSGGQRQTVAIARAVLW
                     NSKVVILDEPTAALGVAQTEQVLKLVRQLADNGLAVIVISHNLNDVFQVADNIAAMYL
                     GSMAAQVEKNSVTQNDVVRLITTGAQK"
     gene            43027..44238
                     /locus_tag="PLW53_01960"
     CDS             43027..44238
                     /locus_tag="PLW53_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36225.1"
                     /translation="MSQDLNIKQSANDWWSKVKAGQVGALPALLGLFVLCIVFGTQSS
                     VFLTPGNLANLLTQAAAVIVIAMGLVFVLLLGEIDLSAGYAAGVCGALLVIMITEMDY
                     PWYLAFPISVLAGILLGGVIGYLVAQLKIPSFVVTLATFLAFQGLLLLLVGEGGTIRI
                     EDPVILAVENKNLSVSYSWIFFLLTAGAYLLSGLLKFRKRKKAGLVDNLFKLWVIKTF
                     VLIAIGTLATWALTVERSNNPDLVSLRGIPYVVPVILVLLVGATFVLSRTSFGLHIYA
                     VGGNAEAARRAGINVKMVRITAFMICTGFAAVAGMIFVSRANSISPTTGGSSTLLYAV
                     GAAVIGGTSLFGGKGKVSDAILGGIVVAVIDNGMALLGYPAGIKFMVTGAVLLISATI
                     DAVSRRGASTS"
     gene            44314..44395
                     /locus_tag="PLW53_01965"
     tRNA            44314..44395
                     /locus_tag="PLW53_01965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44347..44349,aa:Leu,seq:tag)
     gene            complement(44451..45767)
                     /locus_tag="PLW53_01970"
     CDS             complement(44451..45767)
                     /locus_tag="PLW53_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD36226.1"
                     /translation="MSENQDWSFDTQQIHSGQTPDPTTGSRSLPIYQTTAYQFRDTAH
                     AANLFGVAEAGNVYTRINNPTQDAVEKRLATLEKGIASLLVSSGMAATALAIMNVAEA
                     GDHVVASPYVYGGTYNLFKYTLPKFGIQFTFVEDASDMDSWKRAVKPNTKAFFGEGIS
                     NPLGVALDIEKIANVAHEAQVPWIVDNTIATPYVTRPFDYGADIVTHSATKFLSGHGS
                     AMVGAIVDSGNFDFSKNPEKFPGFNQPDGSYNNMVYAKDLGVGSSFGNVAYIFKCRLQ
                     LLRDLGPSVSPFNAWLLAQGLETLSMRMDRHLQNALAVAQWLAKHNQVESVSYSGLPS
                     SSWHSNALKYAAKGAGAVMAFDIKGGLEAGKKFVEGLSLFSHVANIGDVRSLVIHPAS
                     TTHSQLGEDGLRSARITPGMVRLSIGIEDISDIIADLEKGFAAAKI"
     gene            45932..47215
                     /locus_tag="PLW53_01975"
     CDS             45932..47215
                     /locus_tag="PLW53_01975"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRD36227.1"
                     /translation="MTKASSQDSSRAKSPSLESPLATALSQLRRAIDQLKLSENDWNT
                     LSTPRRILEVAVPLRRDDGRVEMYKGYRVQYSTTRGPSKGGVRFHQDIDLDETVALAM
                     LMTWKCALTNLPYGGAKGGVAVNPKTLSLAENERLTRRYTSEILPIIGPERDIPAPDV
                     GTDERNMAWMMDTYSVNAGFSVPGVVTGKPIVLGGSLGRTSATGDGVAISVREALKLK
                     GIDPKGATVAIQGFGKVGYWAALALEEMGLKVVAVSDVSGGVTGFKSVTDLHKYVQEN
                     GTLSNAPGTDRLTNTELLHLGVDVLVPAALSDAITEATASGIKAKIVVEGANAPTTPS
                     GDAIMNENGVLVVPDILANSGGVIVSYFEWVQDKQNYFWSAEEVKTNLSTILMRAITE
                     VADKAKDEKLTWREAANMIGVARVAQAHRLRGLYP"
     gene            47319..48134
                     /locus_tag="PLW53_01980"
     CDS             47319..48134
                     /locus_tag="PLW53_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36228.1"
                     /translation="MADKNFLSWVGFKGEESDASKTNLATGSASLERIRELESQLNDL
                     RSRRDITGLTKEEFEILATETAMSIIRTAQQREAKANSSAEKVLNESKKLASSTLEQA
                     EAKAKSLLSQAESRGRKYIEAAESDASDIISKATSEAEDLLASKKREGGAITANAKRE
                     AERLILDASTEVSEYRNWLAGVISEAERLYRIQSQSLDSAENAISQSRQRLESAFNRL
                     AELQKQVVSAISADGTPSADAKVAAKISAAKNGEAKSAAKKAPSKKSATKKRR"
     gene            48134..49882
                     /locus_tag="PLW53_01985"
     CDS             48134..49882
                     /locus_tag="PLW53_01985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRD36229.1"
                     /translation="MSSSARYIHSVDGLRAVAVLAVLLYHLGIDWIPGGFLGVDLFFV
                     ISGYVITGLILDSIERSGTLDLRAFYLSRIRRLLPALVAMIIFTLLFIGVYAPETVRR
                     FVSDIGYVLSGSMNWALVARQQDYFESIGRPPLLQHTWSLAVEAQFYLIWPLVLLFIL
                     KYFGKKRVSIVALAIAIASGVALFAYSVQIDTQASAISHVYFGTDTHSIGLFLGSALA
                     VSWKPANLTREISKRAQDFIDLIGVVGLLGLLGTFLFINESDPTLYRIAFPLSALFGC
                     ATLISVVHPASRFAPILSTKALIWIGERSYGIYLWHWIVFQLTRPSLDLVGDDWALYA
                     LRVLIVFALADISLRYIEIPVRRGYFELWFRGMKYRTKAVRIRQKLSTILTVVALLGA
                     TTLVANDGIRKADEIAAQEKLAAEVSEPVDQVITNPDSPGLWVTGDSVILGIRNVLAQ
                     FERIELINARVGRQIGELIEVARTDQQFVGASPVVLNLGNNNRLVERDVVELMEVVKN
                     QPKIIVVNTAVPRSWKGENNALIKSVTSRYPNTILVDWDSISANHPEFFASDGVHLNP
                     PGVNAYVSAIREVLQK"
     gene            complement(49933..50685)
                     /locus_tag="PLW53_01990"
     CDS             complement(49933..50685)
                     /locus_tag="PLW53_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36230.1"
                     /translation="MAAKKKASAKRRVKKAPAKRPAKKAAKRVVKKASKRAPKKAAAK
                     RPAKKSAKKASSRNDLSKIVIPPVPTRGATSVSSTPAPAKSASAPAAQAPAKKSNSVL
                     FLVIAGVVLLAIFALTNSSSDDDDAMPTPTETVSEEPSAEPSESESEEPAITAYEAPT
                     GFVAIRNASGGVTLRWKAPAASDGLTGYAVSVSYNAVDFVEVSTVGADQLSFDIALAS
                     DEGGTQFLLQSVYADGTKVDAAKFSLKGKYQN"
     gene            complement(50789..51400)
                     /gene="rdgB"
                     /locus_tag="PLW53_01995"
     CDS             complement(50789..51400)
                     /gene="rdgB"
                     /locus_tag="PLW53_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRD36231.1"
                     /translation="MSKEKKTLVLATRNKGKVLEVERMLKIHAPEISLISMADLDIGD
                     IDETGNSFEENALLKARTVTQISGLAALADDSGLAIDSLNGEPGIYSARYSGVHGDDA
                     ANNAKVLKLMAGITKRSASFIAVLALTRPDGQSIISRGELLGQIIDSPRGDQGFGYDP
                     IFQPTGFDITTAQMSPEMKDSISHRGKALAQIAPKISPFLSLS"
     gene            complement(51408..52139)
                     /gene="rph"
                     /locus_tag="PLW53_02000"
     CDS             complement(51408..52139)
                     /gene="rph"
                     /locus_tag="PLW53_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRD36232.1"
                     /translation="MSEVKRSDKRSPEQLRPITFERNWLNNAEGSVLVSFGNTRVLCV
                     ASFTPGVPRWLVGSGKGWVTSEYAMLPRATHSRSDRESVKGKLGGRTQEISRLIGRSL
                     RAIVDFKALGENTIVLDCDVLQADGGTRTAAITGAYVALSDAISWAMKAGHIAGKSPL
                     IGSVAAVSVGIIDGEPMIDLCYEEDVRAQTDMNIVCTGEGKYIEVQGTAEGEPFDRAL
                     LDKLLDLGANGCKQIASLQAQALTR"
     gene            complement(52164..52967)
                     /gene="murI"
                     /locus_tag="PLW53_02005"
     CDS             complement(52164..52967)
                     /gene="murI"
                     /locus_tag="PLW53_02005"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRD36233.1"
                     /translation="MANLDSSAPIGIFDSGLGGLTVARAVIDQLPNEALIYFGDTARG
                     PYGPRPLAEVRSFALEIMDSLVNAGVKAIVIACNTASAATLRDARERYSIPVIEVIQP
                     AVRRAVAASKSGKVGVIGTQTTIDSKAYNDAFAAAPHLELTALACPKFVSFVESGMTS
                     GDEVTKVAQEYLAPLKAAGVDTLVLGCTHYPLLTGVISYVMGSEVNLVSSAEETAKEL
                     YKVLVENSLLRVEKSAPSHQFISSGDTDTFAHLARRFLGPEVNFVKGQI"
     gene            53248..54024
                     /locus_tag="PLW53_02010"
     CDS             53248..54024
                     /locus_tag="PLW53_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin-like"
                     /protein_id="HRD36234.1"
                     /translation="MQLDASQWSTVYNILSFGLVSMLATTVYTLVSQNRVLPKYRNAL
                     VLSSMVTFIAAYHYLRIFDSFKDATKEGTFEVVTDYNVSSMAFNEGYRYVDWILTVPL
                     LLVEVIAVLALAKEAARSLITRLVPASAAMIALGYPGEVSADQGEKIMWGVLSTIPFL
                     YILYVLFVELGKSLDRQPEGVAATVGRLRLLLIATWGVYPIAYLLPIIDADGAASSGA
                     FVGRQVGYTIADVAAKCVFGLTILKIARMKSIAEGMKDDH"
     gene            complement(54075..54701)
                     /locus_tag="PLW53_02015"
     CDS             complement(54075..54701)
                     /locus_tag="PLW53_02015"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HRD36235.1"
                     /translation="MSSFKREENGEISIEISTQESHVLISLTEQLLELLSQGDSLAEA
                     QDPLMHIVGISDSDALPDDPVLRRLFPDAYEDSASSSEFRRYTEYGLREKKKAHARII
                     YEALTSQGEPEEITGGNTPIDKGSFKLIINSAELLDWLSGLNDLRLALAVRLGIGGNR
                     QESADIDTKAIHNKYELMLESDPMKAVYAVYSWIGWLQQGLLEEVSPE"
     gene            complement(54706..54996)
                     /gene="clpS"
                     /locus_tag="PLW53_02020"
     CDS             complement(54706..54996)
                     /gene="clpS"
                     /locus_tag="PLW53_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRD36236.1"
                     /translation="MALGTATQVDSEVNLKADRPWVCIVWDDPVNLMTYVTYVFMVLF
                     GYSKEKATELMLKVHNEGRAQVSSGTREEMERDVQRLHEYGLWATLQRDNEY"
     gene            complement(55004..55318)
                     /locus_tag="PLW53_02025"
     CDS             complement(55004..55318)
                     /locus_tag="PLW53_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HRD36237.1"
                     /translation="MKQAFELKQVMGLFGIGKPSSDGDTLIDTRTQEQLDSGDHERFS
                     HYVRKEKIVESAVSGKPVTALCGKRWIPSRDPNKFPICPTCKEIHAGLRKGPDDQSNN
                     EL"
     gene            55384..56325
                     /gene="argF"
                     /locus_tag="PLW53_02030"
     CDS             55384..56325
                     /gene="argF"
                     /locus_tag="PLW53_02030"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRD36238.1"
                     /translation="MKDLLRSQDLLKSDVEMLLATASEFASEPLKASNLLANKTVAIY
                     MSKPSTRTRLASESAVAHLGGTPIFLRGDDLQIGRGETIADTAKVISQFAQALIIRTF
                     AQSDVDELGANASIPVINGLTDYDHPTQALADWLTIREVFGKDISGRKFAYFGDGNNV
                     TTAWLMMGAIMGAHVVAATPEGKFAPKKEIVELASKLALESGGKVEVTSDAKSAASGA
                     SVLYTDVWMSMGDPEADRIEKEKTLSSYSVSDELMGLASKDAIFMHCLPAHRGQEVSA
                     SVMDGPASKIWRQAFHRRTTIQAILYHCLRGELKGRN"
     gene            56395..57510
                     /locus_tag="PLW53_02035"
     CDS             56395..57510
                     /locus_tag="PLW53_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRD36239.1"
                     /translation="MRVVVPAEGKAGEKRVALLPDIISKLTKAGLSVSIESRAGIHAG
                     ASDESYIEQGATIFGASDQRKELAGADVILSVQPLTPDQASNLKSGAITISFLSSVSA
                     KESIDALAKSGATAFSLEALPRISRAQSMDALTSQALCAGYKAALVAAELSPRFFPLL
                     MTAAGTVTPANVLVLGAGVAGLQAIATSRRLGAVVSAYDVRPSSADEVKSMGATFIDL
                     ELEALEGSGGYAREMSEERAAKQRELLTPYITKSHVLITTAAVPGRAAPMLVTKQMYQ
                     AMAPGSVIVDLAAESGGNVEGSKPGEVITVGNSVLLWGGKDVPSQLPFHSSYLLSRNM
                     VNLLLLMIKDGAIALDFSDEIIDASALTHGGNLRKGS"
     gene            57524..57820
                     /locus_tag="PLW53_02040"
     CDS             57524..57820
                     /locus_tag="PLW53_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRD36240.1"
                     /translation="MAIISIFTLSIFVGFEVVSKVSSTLHTPLMSGANAIHGVILVGA
                     IIVADHSDNSLELGLSLAAIVLATINMVGGFVVTDRMLEMFKGKGAKSKGSGDK"
     gene            57821..59209
                     /locus_tag="PLW53_02045"
     CDS             57821..59209
                     /locus_tag="PLW53_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRD36241.1"
                     /translation="MSRFLYELIGLSAGVMFILALKGLSHPRTARRGNLLGAAGATIA
                     TVAVFFYSSDGELPLNNLGWILGAIAFGLIIGVPAARRVQMTAMPQLVALFNGVGGGA
                     AALVAIVEYLKLGQSASTTVVIATVFTVIVGSTSFSGSIVTFLKLQELMTTRPVVFAG
                     GRFVIAGTLLATLGCAGWVVSSLGTTPLLVLAGLSLAFGILFVLPVGGADVPIVISLL
                     NAFTGLTVAAGGYVLDSTLLIIAGTLVGASGTILTRLMAEAMGRSLFGTLFGAFTAKP
                     QDSSGAGEDRPVKSGSPDDVAILLNYARRVVIVPGFGLAVAQAQHTVRELADLLSAKG
                     IDVAYGIHPVAGRMPGHMNVLLAEANVPYEQLSEMDEVNPTFPQTDVALIIGANDVVN
                     PAAKTTPGCPIYGMPILDVAQAGNVIFLKRSMRPGFAGIENELLFDSKTTLLFGDAKA
                     SLTKVVSALKAL"
     gene            59244..59516
                     /locus_tag="PLW53_02050"
     CDS             59244..59516
                     /locus_tag="PLW53_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36242.1"
                     /translation="MESLALLVSIIVFPAMFGGPIALLLSLYRPAGMSNFRRIFIILF
                     AILSIIVGIFLLIENVSRGANVIGALGLSTGLGSLWQLKRYRKQTP"
     gene            complement(59538..59913)
                     /gene="ssrA"
                     /locus_tag="PLW53_02055"
     tmRNA           complement(59538..59913)
                     /gene="ssrA"
                     /locus_tag="PLW53_02055"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(60011..60517)
                     /gene="smpB"
                     /locus_tag="PLW53_02060"
     CDS             complement(60011..60517)
                     /gene="smpB"
                     /locus_tag="PLW53_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRD36243.1"
                     /translation="MVKEIGKKLIAQNKKGRHDYFIDEVFEAGLVLVGTEVKSLRAGR
                     ATLTDGYGMVQDGEIYLSGVHIPEYNEGSWTNHEPRRNRKLLMSKREINRLIGTLKES
                     GLTLIPLSLYFKDGKAKVEIALARGKKAHDKRATLMEKQANREIDRELSHRSTGKSSR
                     GKSKRYED"
     gene            complement(60572..61843)
                     /locus_tag="PLW53_02065"
     CDS             complement(60572..61843)
                     /locus_tag="PLW53_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRD36244.1"
                     /translation="MSEKKKSPASKRAVIGGRARKPDSTATRSKTVSEVNPSKVIDSP
                     EAINSRQRITNPDQLRKAVLAVLITSLVFAIGIQVGESRNSSSVDEAIKSVVESGAKD
                     IDRDVLERAAIEGVLKASGDEWANYFPREALETFEAQSSNSFTGIGIYLSKSRGGVIK
                     ITSIQEGSPAANSDLAIGDELVEVNGTQVTGASLASTIALIRADIGKRVDLLVVRNEQ
                     RILASIKTGKVQAKSVELTQVSSSVALVEIASFISGTANEVSEALKSANYKSGVIIDL
                     RNNSGGLVEEAVAVAEQFIGSGIIVSYKVNESEKVFRAVNSNPIKVPVVVIINRNTAS
                     SAEILAGAFQDRNRGVVIGERSYGKGSVQELISLDDGSKLELTVALFLTPSGRIIEEV
                     GITPDLAVAPAEIGLKSLQVLGGLASFISKD"
     gene            complement(61970..62872)
                     /gene="ftsX"
                     /locus_tag="PLW53_02070"
     CDS             complement(61970..62872)
                     /gene="ftsX"
                     /locus_tag="PLW53_02070"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRD36245.1"
                     /translation="MRAGFILSEVGIGLRRNLTMTFAVVVTVAISLSLLGIGLLSNSQ
                     VRVMKDYWYDKIEVSVYLCGSLSDSPSCAGGIITSEQRLEIQADLEELPVVETVYYES
                     QSEAFERFQERFKDSAISQNVTADQLPESFRVKLTDPTQFAVVQSAFSGRPGVDVVQD
                     QRSILEKFFRLLNVLRDGALAIGFASVLTAALLISNTLRIAAFNRRRETNVMKLVGAS
                     SFSIQLPFILEGVFSAIIGWGFSVGLLSSLKYVIDDRIAPLLTFTNFFTWNDVWVVSA
                     YLLGTGLLVSSLASILTLRKYLKV"
     gene            complement(62875..63564)
                     /gene="ftsE"
                     /locus_tag="PLW53_02075"
     CDS             complement(62875..63564)
                     /gene="ftsE"
                     /locus_tag="PLW53_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRD36246.1"
                     /translation="MIRFENVTKGYAKNDRPALDNVSIAIEKGEFVFLVGLSGSGKST
                     FLRLVLREERPTSGTIHVAGKDLGTLSSWKVPELRRQVGTVFQDFRLLPNKTVAENVA
                     FTLHVLGFSKKEIAREVPEVLELVGLEDKSDRKPGELSGGEQQRVAIARAYVSNPAIL
                     IADEPTGNLDPATSVGIMKLLDRINREGTTVVMATHDSGIVDQMRKRVIELDKGQVIR
                     DQVRGVYGYTG"
     gene            complement(63667..64770)
                     /gene="prfB"
                     /locus_tag="PLW53_02080"
     CDS             complement(63667..64770)
                     /gene="prfB"
                     /locus_tag="PLW53_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095687825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRD36247.1"
                     /translation="MAERDINGEIAALDATLKSIEAVLDLGKLRKEQVELETQAGVPD
                     LWSDPEAAQKVTSALSRVNSTINKVSSLRSRVTDLPIMLELANNESSDGAELEILKEL
                     DSLTSAISDLEIQTLLSGEYDHRDALITIRSEAGGVDASDWAEMVSRMFFRYCERHNY
                     GVSVLETSYGEEAGIKSTTFKVSGPYCYGTLSVEQGTHRLVRISPFDSQNRRHTSFAG
                     VEVVPVVDQSDDIEIDEKDIRIDVYRSSGPGGQGVNTTDSAVRITHIPTGIVVSCQNE
                     RSQIQNRATAMAVLQSKLLERRNAQERAKIDALRGDTSGSWGNQIRSYVLHPYQMVKD
                     HRTDFETGNTQAVLDGDLDGFISAGIRWRRERK"
     gene            complement(64805..65323)
                     /locus_tag="PLW53_02085"
     CDS             complement(64805..65323)
                     /locus_tag="PLW53_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36248.1"
                     /translation="MLINGVMRVRTQLEDEYGSISPLIMGYFIIAMSLTFLISNIASV
                     YIARRDLINLTEGALSRAVQELDEIAYYYQVPIPETFLGQSRAVPINCSDAGRTFSQE
                     VALIAKSKLDLYGRSFNQANSDIELKSFICDGKSLHVSLQQVHQLPFKLRVFGITEFT
                     NQIEVSMESIYQ"
     gene            complement(65295..65747)
                     /locus_tag="PLW53_02090"
     CDS             complement(65295..65747)
                     /locus_tag="PLW53_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36249.1"
                     /translation="MNRNSDESGSAIAEFVLIATPLFIPALIFFSSMQQSAKEEMTVS
                     TLARQSLRAFITADNLSLGHQRIRFLLDQYQEVENKGRNSDDLRPGFTYNISCQSQGC
                     LKPGSLVELSLYRSFEIEVHDFDKSVAGENRKAAAVVRGYVDKWRDES"
     gene            complement(65740..66099)
                     /locus_tag="PLW53_02095"
     CDS             complement(65740..66099)
                     /locus_tag="PLW53_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36250.1"
                     /translation="MKCSDNHDLESGSVESALSLIPTTAFFLLVLQLMLSGSWQLLDK
                     IDISNAINRTALFSAEAGKTAFYALDGRREIDQEVLALPGGGEIITYSSTLDIPVITN
                     LSGSAIKMKSEGIAIRE"
     gene            complement(66099..66293)
                     /locus_tag="PLW53_02100"
     CDS             complement(66099..66293)
                     /locus_tag="PLW53_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36251.1"
                     /translation="MNKSKVAEKYREFMAKDVDESGDVPGWVLVVLMTTGLVTAIWTI
                     AAPRLSTILKNSLDAMNGIR"
     gene            complement(66472..67260)
                     /locus_tag="PLW53_02105"
     CDS             complement(66472..67260)
                     /locus_tag="PLW53_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD36252.1"
                     /translation="MKAELTDIRLLSLLSVLPLMLLGVWIISNESFANLGSRSEKYSR
                     VIRVESLGHKASRVRDIGIYFLILSAATIALYLLIAIWPEQIPASLLLLSILALFLAL
                     LRKRADTERRVATEIFERELPSLIQLLTILISSGISPSRALAIIGQRSTSLSARELDL
                     VVSEISNGTSLTQALDNLALRYDSLVLRRFTTSIVLGIERGSSLGPILISQVKDARLA
                     SKNLILQRAGKAEIALMIPIVFLILPISILFALFPSFEQLNSFM"
     gene            complement(67257..68081)
                     /locus_tag="PLW53_02110"
     CDS             complement(67257..68081)
                     /locus_tag="PLW53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD36253.1"
                     /translation="MFEQFALPVFTALLAALGSYIALFDLSSLNKRLKPDKKISSRSV
                     ITLIITLISSYFTTFLLTSSEAISIAIATLSAALPFLIGRGRNLKRQREQEVAWPEAI
                     DSLVSALQAGISISEALTQLALHGPKVLRPSFAQIQSNLLSLGNFEQVLLKEKRRLDS
                     AISDQVFETLIISKDFGGRDANNALRLLAEFVREDIAVAEEIRTKFGWIRNSALLATA
                     APWLLLILLSTQDSTVEIYSTPSGALVLSLGVVMTATAYIWMEKVGSLPAAPRALR"
     gene            complement(68074..69063)
                     /locus_tag="PLW53_02115"
     CDS             complement(68074..69063)
                     /locus_tag="PLW53_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRD36254.1"
                     /translation="MENEETHQGADYGALQSLLEDDEVEEIWINSPQRIFIARSGKSE
                     LTNIVLSASELQSIMDRILMWSGRRIDLSHPFVDARLPDGSRLHIVIPEVTSEHWAIN
                     IRKHLMRGKSLLNLVEMEVISSEIREFLGEAVRDRKNILVSGSTQAGKTTLLNALVSA
                     VPITDRVITIEEVFELSPRIPDCVAMQTRSASLDGVGEISIRKLIKEALRMRPSRLVI
                     GEIREAESWDMLIALNSGIPGMATIHANSATEAIRKLQTLPLLAGENITQDFLTPTVF
                     RALDYVIHVGLDSTGVRRVLQVVKVLDRAENFHIDLEPIFTWSQGQYQRGFHV"
     gene            69203..69278
                     /locus_tag="PLW53_02120"
     tRNA            69203..69278
                     /locus_tag="PLW53_02120"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69236..69238,aa:His,seq:gtg)
BASE COUNT        18971 a        15968 c        14869 g        19549 t
ORIGIN      
        1 aggaggcgct cttgggataa tttctcagct ttcatcactt ccccacttta ggctacgagg
       61 ggaaagtatc tataactagc gctcccctct ctgctgattt acgcttactc tcgtgatgag
      121 aagaaatctt gcagccctta tctcacttac tctgaccctt tggattcttc ctgccaccac
      181 tgccgcagct gcaaatccaa aggctggggc aaagtgcagt aaggcaaaac aagtaaagat
      241 ttataagggc aagagttaca cctgcataaa gtcaggaaag aaactgactt ggagcaaagg
      301 caaaactatt tcttctgtag aaaatgcatc gacgcctacg ccgactccaa caccgactcc
      361 aacaccgact ccaacaccga ctccaacacc gactccaaca cctactccga ctccgacgcc
      421 aaccccaact ccgactccga ctccgactcc gacgccaacc ccaactccaa ccccaactcc
      481 gactccaacg ccaactccaa ctccaactcc gactccaact ccaacaccaa caaaagtcgg
      541 ttacacaatg gatcaggtta aagctaataa cacaagtgca agctgctgga cggtaattga
      601 taactacgtt tacaacttaa ctaattggat ttcttctcac ccaggagggg ctggggcgat
      661 tcgctcactt tgtggagttg atggcactgc ttcctttaaa gcacagcacg caaatcaatc
      721 caaccctgca tcgcgtctaa actcttatct tctagggccg ctatctaagt agatctttac
      781 ttctttagaa actggagatt aagtttaatc atcttaagaa gtaacttctc atctaccttc
      841 caatcaacgg gtacttgaac cgtcttctta tttactttgt agccctctaa atcctgcttc
      901 atcgccaata aaaccttctg gttccagggc gctataagta tgtggttttt aagaattgat
      961 gctccaaaca agtactcatt acccaatttc aacattggct gattccaagc aattacaagc
     1021 tctaattttg gatatttctt ctgaataact ctaaatatct tcttaatagt ttttctcttt
     1081 acttcatcct ggccctttag atatccctct agatcatcaa agcgcttaga gctcttcgat
     1141 cctctggaat acatcactag ggcatttgcg tgagcctggc taaagccatg ttcttctctt
     1201 aagtgagaga tttgctgcgg gtatttaagg tcagagatat ctttcataac agcaaaccaa
     1261 tagcgcattg gttggccata cttggcttct atctttggaa agtgggcctg gcgacttcct
     1321 ttatctttta ctgccactat tttttagcga ggccctgcag ctctatgacc gcagccttgc
     1381 ggattactac agcagcaagt gcaattgcct tctgggcagc cacaggggca tttgaaatct
     1441 tggcttgcat cactcattga tctaatcctt atctatcaag ctagtaaatg cccaacccca
     1501 agcaaggcag aagagaaaaa ctccgagaat ataaagtaag tcgctcatgt gcagatacta
     1561 ctttgtaaat caatcaagag caggataaac ggtcaataga caaggagttg tgggcgctga
     1621 gggttttgaa cccccgacct actcggtgta aacgagccgc tctaccgctg agctaagcgc
     1681 cctatcaaaa tacttgcgca gtctataacg aggctagcgc gcttctgtaa tcctcgattt
     1741 ttctcgcctc agcagggcta tttattacgc tccagcgcac aattcctgcc ttatcgataa
     1801 taaatgtgcc gcgtagcgca agaccgcgat cttcattaaa tactccatat ttcttcgcaa
     1861 ctgcgccatg gggccagaag tcagagagaa gtgggaagtt atagccctcc ttttctgcaa
     1921 agactttctg ggtaaacatc gcatcacatg agattgcaag aagttcgata ttttcatttt
     1981 ggaaagccga tagatcatct cttagcgcgc agagctctcc ggtacaggtg ccagtaaagg
     2041 caaaaggaaa gaagactaga actacattct tcgtgcctct aaatgaggag agtgaaactt
     2101 tctctccaaa ttgatttacc aattcaaaat caggagcaag atctccaata cttattgcca
     2161 ttattttctt ccactctttc gcgcaaccaa tctagttgca gtccaatcct ttgatgctgg
     2221 aattgttgag gtaaggctta agcctgcagt ctgagcagca tcttgaatat cacttggctc
     2281 aacatgtcct ggccttgaaa cctttggtgt tagaagccag ataggtcctg attcacttaa
     2341 gtaggtaagg caatcgacta actcatcgat tagatcgcca tcatcttcgc gccaccaagt
     2401 aataacagca tctactacct cttgagaatt ttcatcaagg aattcacttc cagtagcagt
     2461 tgagatttcg ctacgaattg cttcatcgca atcacttcca aagcctcgct ctaatacgag
     2521 aaaaccgctc tggattccca ttcgggatgc cagttgccct ggccccaccg gcggggtgct
     2581 caatgccttc tccttaacat tcttagctcg ctcaaaagtt gagccttatg ggctaagtcc
     2641 acactcttaa gggctagaaa gcaacttggt aaaaggggca aactcacact ccttggattt
     2701 cactcaaagc gccatttaag gcaaagatag ccctgtgagc ccaggcccag agaatatgaa
     2761 taatctccaa gacctccatg tggatcagct aatcgatgcc aatcccgatg aaaccgccga
     2821 atggcaccag agccttgatt ccttaattaa gcatgccgga ccagcgcggg ctcgctacct
     2881 aatgctctca ctaattaagc gcgctcatga ggcaaatatt caacttccaa atctacgtct
     2941 taccgattac atgaacacca ttccacctga gagtgaaccg caatttcctg gtgatgaatt
     3001 tctagagcga agaattcggg catacatcag atggaatgca gcgatcatgg tccatcgcgc
     3061 ccagcgccct ggagttggcg ttggtggaca catttcaact tttgcatcat ctgctgcgtt
     3121 atatgaagtt ggttttaatc acttctttaa aggaaaagat catccaggcg gcggcgatca
     3181 gatatttttc caaggacatg catcacccgg aatgtatgcc agagcatttc ttgaaggacg
     3241 acttagcgag caccaattag atggatttag acaagagctc tcacactcag gtggcggact
     3301 ctcctcctat ccacatccaa gacttatgcc agagttctgg gagtttccaa ctgtctctat
     3361 gggacttggt ccgattaatt caatttatca agcccgtttt aatcgctatc ttgctaaccg
     3421 cggaattaaa gatacttcgg gccaacatgt ttgggcattt cttggcgatg gcgagatgga
     3481 tgaaccagag agcgtttcag cgctaactct tgctgctaga gagaatctag ataacttaac
     3541 ctttgttgtt aattgcaatc tacagagact tgatggaccg gtgcgcggca atggaaagat
     3601 tattcaagaa cttgaagccc tatttactgg cgctggatgg aatgtaatca aagtagtttg
     3661 gggaagagag tgggatccgc tctttgctat ggataaagaa ggcgcgctgg ttgagcttat
     3721 gaataagacg ccggatggag atttccaaac ttataaggca gaaagcggcg cctttgtgag
     3781 agagaatttc tttaatcgcg atccaagaac agctgcgatg gtggctaatt ggagcgatga
     3841 tgatatttgg aatctaaaac gcggcggcca tgattatcag aagctctacg ctgcttatca
     3901 atcagcgatg aaccataaag gccagcccac tgtaattctt gctaaaacca ttaagggctg
     3961 gaccctggga tcacattttg aaggacgaaa tgccacccac cagatgaaga agctaaagct
     4021 tgaagatcta aagtccttgc gagatcgtct ctatctagaa atagatgacg ataaattaga
     4081 tgaaaaactc ccaccatatt tccatcccgg aaaagaatct ccagaattcc aatacatgat
     4141 ggagaagaga gcagaacttg gtggatccgt cccaagaaga cgatctaagt caaaacccct
     4201 tccccagccg caagattcag attttgcagt gatgacaaga gggtctggag cgcaagaaat
     4261 tgccaccaca atggcctttg ttcgcctact taaagattta gcaaaagttg agggacttgg
     4321 acatcgaatt gttccaatca ttcctgatga agctagaact tttggtatgg attcactctt
     4381 tccaacaatg aaaatctact caccacatgg ccagcaatat ctggcagtag atagagagtt
     4441 gatgctttct tataaagaat ctacctctgg agtcattctg catgaaggta ttaatgaggc
     4501 tggatcaact gcttcattta ccgctgttgg tacctcctac tcaactcatg atgaaccaat
     4561 gattcccgtt tatattttct attccatgtt tggtttccag agaactggag atgcattctg
     4621 ggcagcagca gatcagatgg cacgtgggtt tgttatggga gcaaccgctg gaagaacaac
     4681 tcttaatggc gaaggtttgc agcatgaaga tggacactcg caacttctag cctcaaccaa
     4741 tccagccgtc gttgcctatg atccagcatt tgcctttgaa ctaggccata ttgttaaaga
     4801 tggtttgaga agaatgtatg gcgaaaatag cgagaatatt ttctactacc tcactgtcta
     4861 caacgagcct tatgttcaac cagctgagcc agagaatcta gatgttgaag gtttattaaa
     4921 aggtatctat ctctattcaa aggctaaaaa gcagcgcaga gctaagcgcc aagttaatct
     4981 gctggcttca ggagtagcac ttaattgggc ccttaaagcg cagaatctgc ttgctagtga
     5041 ttggaagatt agcgctaacg tatttagcgt cacttcttgg aatgagctaa ggcgagatgg
     5101 cctagaagtt gatcgccata atctgctaaa ccctctagat aagaagagtg cttacctcac
     5161 aacaaaactt aaaaactata aaggaagcgt tcttgcagtt agtgattaca tgagaacggt
     5221 tcaagaccaa attgcgccat gggtggagca gaactttgcc tctcttggaa ccgatggctt
     5281 tggtctatct gataccagag gcgcactacg tcgccacttt aaagtagatg ccgagtcaat
     5341 cgttgtcgca gctctttcac aacttgctaa tcaaggcaaa atctctccta aagttgtcaa
     5401 gaaagccatt gaaaagtatc agttgaacaa tgtcactgct gcagatccag gaaatactga
     5461 aggctctgga tgatttctcg ctttggaata attgatatct ctccagcaac tttctttggt
     5521 ggagaattta tcggtgccaa agctgtagtt aatgaggcag ttgcaatctc tgcaaccata
     5581 atcagagaag gtcatgaaag ctttgatgca tttgctgttt tagttgatga aagtggcaag
     5641 attgcttcgc gcgagaagat ggttgagatt tggccaaata gccagagatt tgaggccttt
     5701 ctaagaccaa aggctttagg taattggagt ttttatattg aggtaagtgc agaaaatcct
     5761 ggagagtttg ctaaaactct aatgacaaaa tcagagagtt atccaattaa agtcgagcga
     5821 gagcgggccc tagttggcaa ttggtatgaa ttctttcctc gaagcgaggg cgctaagaaa
     5881 aatagtgatg gctcaataag ttctggaaac tttagaagcg cagcagaacg aattgaagat
     5941 gtagccaaaa tgggttttga tgttttatat ctcccgccga tacatccaat tggatactcc
     6001 catcgcaagg gaagaaataa ttcacttatc gcaggcccac tagatccagg ggttccttgg
     6061 gctattggca atgaatcagg aggccatgat gcaataaatc ctgagcttgg cagccttgat
     6121 gactttaaat attttcttaa gattgccaag aaaaacaatg tggaagttgc acttgattta
     6181 gccctgcaat gctcccccga tcatccgtgg gtaaagacaa atccagagtg gtttacaaag
     6241 agggctgacg gctcaattgc ttatgccgag aatcctccaa agaaatatca agatatctat
     6301 cccctaaatt ttgataatga ttatccaggg ctctttgcag aaatctatcg agtagtgacg
     6361 caatggatag aacttggaat aaatatcttt agagttgata atcctcatac taagccaatt
     6421 aatttctggg cagattttat tgcaaagata aatcaaaaat accctcaagt tgtttttctt
     6481 gcagaggctt tcacaactcc tgcgatgatg catgcccttg gtaaagctgg ttttcagcag
     6541 tcctatacct atttcacttg gcgagttacc aagcctgaat tagaggaata tgccaaggaa
     6601 gttgcctatc aaaccagcgc tttctttcgc ccaaatttct gggttaatac cccagatatt
     6661 cttccctttc atttgcagag cggaaatcct gcagcctttg cgcttcgagc agttctagct
     6721 tcaactctta gcccatcttg gggaatgtat gccggatatg aactctatga acacattcct
     6781 attggtgagg gtaaagagga gtatcgagat agtgagaagt atgaaattaa agtacgcgac
     6841 tggcaggctg cagccaagaa atctctaact ctggcgccat ttatcaccaa gctaaatgag
     6901 attaagaatc aaaatgttgc ccttcagcgc ctaagaaata ttacttttca tcagagcgac
     6961 tctgatcaag taatcgccta ttcaaagaaa gagggcgata atctcattct ggtggtagtt
     7021 aatttagatc cattcaaagc cattgaaacc atggtccatt ggaatctttc agcgctcggc
     7081 ctttcagata agggatttga ggttaccgac cttatagatc aagcaaaata cagttggagt
     7141 agcgatactt tcattcgcct tgatcccaca cgtcctatgg gaagagttgc ccatatcgca
     7201 cgcgtcaaga agtaattagg cttacgccct atgcgccgct tgtttaagaa tttattttca
     7261 aagcgaagcg cttttcaatc acctcctgct ggatatttaa atccgcatag cacccttggt
     7321 gagttagata tgtatttaat caccgaagga aggcatgaat cactctggga agtactggga
     7381 gcccatgtta aaagagatga acatggtgag ttgataggaa cggcttttag cctttgggca
     7441 cctaatgcaa aacgagtgag tttgatttgt gatgcaaatt tctgggataa agagattaat
     7501 ccgatgattc ctcttggttc taacgggcta tgggaagttt tcatcaaaga tgttggcccg
     7561 ggacttaaat ataaatttgc tattcaaggc agggattctc gctgggtaga tcacgcagat
     7621 ccgttagcta ggcagagtga gcgaccgcca cacactgcat ctattgttaa tgagagcaat
     7681 taccaatgga gtgatagtaa gtggatggat agccgtggtt tatttcaacc ttggaagagt
     7741 ccggtatcaa tctatgaagt tcatttaggt tcatggcgag ctggactttc atatcgccaa
     7801 cttgctcaag agcttggtca atacttaatt gaatataaat tcactcatgt tgaatttatg
     7861 ccagttagtg aatatcccta tgatccttct tggggctatc aagtaacttc atattttgct
     7921 ccaacttcgc gctttggaag tcctgatgat tttagatatc taattgatca cctacacagc
     7981 cttggaattg gagtcatttt ggattgggtt cctgcccact ttcccaaaga tgaatgggca
     8041 ctagctcgct ttgatggcac ttgtctctat gaacatgaag atcctcgttt aggtgagcac
     8101 cctgattggg ggacgctgat ctttaattac tcaagaaacg aagtgcgcaa tttcctagta
     8161 gctagcgctc tttattggct cgagagcttt catattgatg ggcttcgagt cgatgctgtg
     8221 gcatcgatgc tctatctgga ttattcgcgc aaagagggag aatggattgc taatgaattt
     8281 gggggacgtg agaatttagc agcagtgaag tttttgcaag aagcaactgc aacagcctat
     8341 aaacgctttc caggaatcat gatgattgct gaagaatcaa ctgcctggac tggagtgact
     8401 aaaccaactt ctgagaacgg tttaggtttt gggtttaagt ggaatatggg atggatgcat
     8461 gacagcttgc aatatctatc tcatgaccca attcatcgcc tttatcacca taatgagatt
     8521 accttttcaa ttctctatgc ctttagcgaa aacttcttac ttccactttc tcatgacgaa
     8581 gttgttcatg gtaaagcagc cctagtaaat aaattccctg gagatcgctg gcagaaagtt
     8641 gcaacacttc gcgcgctcta tggctatatg tggtctcacc ctggaaagaa attacttttt
     8701 atgggctctg aatttgccca aaatgatgag tggagccatg acaaatctct tgattggcat
     8761 ttgactcaat atggcgaaca taagggagtt caagatttag ttagagatct aaatgctctc
     8821 tataaagaaa gcccagctct ctggcagaga gatagttcaa gtgatggttt tacctggtta
     8881 gttggagatg atggggcagg aaatactctg gcatatcagc gcttggctga tgatggatct
     8941 caaataatct caattacaaa tttctcccca gttccccacg agcaatatcg ccttccgatt
     9001 cctaagtcag gaaattggat agaggcgcta aacacagatg atgttaaata tggtggatct
     9061 ggcgtaataa ataatcagat aaaagttgac cagattcaac acagaggttt tgattattcg
     9121 gcagttattc gcctagctcc tcttgcaact atctggctaa agcttaatta gcctcgctta
     9181 ggctgacttg ctgctgatac aaagaaagcc acgagaatca acatcagacc aggaattgcc
     9241 atcgcaatag gaagagttgc agcccccgca acaagggaga ttactccgcg gcagatgaaa
     9301 gtgagcacct gattcatggc gccaagttct gcaactgcta ctccacctgg tttgttggag
     9361 cgggcgctgg catagccgaa aaataaagga ccaacgaagg aaattccaaa gccagtaagt
     9421 aaaaatccta gacaaaagat ggtgtatcca agaactaagt tgctctcttt tatctgcatg
     9481 ctaagtgcca gacaggtaag aaatactgtt ccgcccataa ctacgaaggg ttgaacaagc
     9541 tctttatcac tgcgattacc tttgattcga ctataagtca ggcgaccaat aatcatcgct
     9601 cccataaata agaaataagg cgtaactgca acggatttac tcacgccaaa ttcttgacgg
     9661 gccataattg ttgcccaatc gccgattgaa acctcaagaa gtaaagcaca taaaaagcca
     9721 aggctgacaa cccaatcaat ttgaaacgag gtaatcattt ttttaaaggt tatgacctca
     9781 tcatcttcag taacctcatg gccttgcaat agatatgcct tagatgcctt tattgaacga
     9841 agagtaagta aataaaccag tgagattaaa gtcagaatgt gccagttcaa tgaaacaaat
     9901 gatgaaatta agagtgcaat aaatgctgta gtaacggctc ccgcgctcca aaggccatga
     9961 agcataggaa ttatctgttt gccgctttca tgctgacgat gtagagcctg ggcattgatt
    10021 gcgatgtgat aggaagccca agcaaaacca cagaggacat taaccactag ataaatcggt
    10081 gcgcttttta attcaatcaa tatgccaaga gttaaatatg taagtgttga gctatagatc
    10141 aaaactctat aaacccctag tcgatgaacg atgtggccca taaccaattg agcagataaa
    10201 gcaccaattg agcctgtgct catcaagaac ccaaagtagg aggtggaaac atcaagattt
    10261 gctttgatat caggaagcct gggagccagg cacataattc caagtccaaa taggaagaag
    10321 aaggcctgca agtggcgtaa ttcgctacgt tctagaggtt tattcaaaac aaggcgctcg
    10381 ctaactcatt tctagaacgg atcaaatcag gatcggcagg atctactaac tggaaaagca
    10441 ataataggtg ctctttggct ctcttcttct catccccact ggagttttta acaaaattta
    10501 ttagccttgc aaatgcgttt ttctgtagcc cttgagcaat ttcaacatcg gctgccatta
    10561 ccgccttctc tatcgaagtg ggatcactat cggcatcttt gatagtaaga gatggattga
    10621 gggctgagat acgaatcatt aactcgcact gtgcaagtcc aattttggca actgattcat
    10681 caggttttct catgagccaa tttcgataag ccattgcagc tcctgagtaa tcacccttct
    10741 ctaaagcact tagagcagct gcctcctctg gctccattgg aatctcttta acttctggga
    10801 catcaacatt taaaccctgc tctttggcta attcaaataa cttggtaatc accatgacaa
    10861 tctgttctct tggatagatg cggtcaaaga gcgctacagg ttgctcattt ataaatgcaa
    10921 ttgcaaatgg aacagcagta attttaagcg cttgcaccaa ttgcacttga gtatcggcat
    10981 taattgctcc aaaaatccag ctatcgttat cttcttttgc aatacttgcc attaattgtc
    11041 ttaattcaac cgttgccgca cttcttggtg agtaggccag aaggacaaca ggcttttctt
    11101 tagaacggcc aacataatca gccataaagt tttcaacagt tgcctcatca cttgaagtgg
    11161 cccctacttc aacttttggt ttagtcagcg agctcaaatc aaaagctcta ccaaaaccag
    11221 caggaggcat gctcatagcg ttattctctc aaagagtggg agcacctgaa tccattctct
    11281 ctggcaatac ctttgaaatg tagtcattgg tggcgaattc aagacctaaa tctcgccctg
    11341 ccttttcccc caaataccag cgatgttcaa gtacttcatg aaatagctgt gcatcctcta
    11401 ctcgcccgcg cattgcagtt ggaactgcat ttatgacctt atagaaaact tcatcaagcc
    11461 agcgttttgc agcttcttcc ttcttaggag tcttttcaat ttcgcgggat ctaaagcgat
    11521 caaacgaggc caatatccgt tttgcttgta gctcttccgt ttcaaggccc attagttcga
    11581 ttaatctctg tgagtgatag ttaggagcca ctagctttgg aataaaggta accgttgatt
    11641 tatcccctgc taattgaata tcaacttcct ccactgcaaa accaatatct tgaagcgatc
    11701 gcatcgctct ttcgactgca tgtctatctc cagctggcaa gatctgcggt tcacttaagg
    11761 atttccatat ttttcgatag cgagtaatta ccgattcact cgctctaatt ggatccatag
    11821 cgggaaatag aacgccagca atctttaaat cttcaagctc tgcggctaca ttaaatcttg
    11881 caagctctaa atcatgctct cgctgcccat cacttaactt tttttgaaat tctccagttt
    11941 cagcatcaac taaataagca gcaaagccct ctgcatctcg tcgaaacaaa gtgtttgaaa
    12001 gagaacaatc tccccaccaa aatcctagta agtgaaggcg gactaataaa taagccagag
    12061 cattggccat atttgtaatc tcatgttggg ttatctgacc acttaaaata actcgatagg
    12121 gaagtgagta aggcaagaat ctagtcacta ttgcgcaagg aagttcttca ccgttttgac
    12181 tatagcgatt agtgacaatt gctacctgag caacggctgg cgctccccta agacttaatt
    12241 cacgcaaggc ttcatattcc cgcacagcaa actcttcaac tgtttctttt actgcatata
    12301 tttcactagc aggatccggt gttgagcgaa ccaatctgac aatatgacga gaaataccgc
    12361 gttgcgctgt cagagtttca tcttctggcc actgctccaa tggcgtatgc cagggcaggt
    12421 tatatagggc tgagatttcc tcgccaagtc cctttatatg tagttgttta tctgtcgctg
    12481 ccactgctgc attctttcag gctttagact agatgtatgg cgaaaattag aggagttctt
    12541 aaaaaagttg gagcaagacc atctaaagtt caagaagttc aagattttgg aaaaaaaggt
    12601 gctccactta acacgcaaca ccccttctat tttggctttc ttgcagcctc aggtgcctta
    12661 gtagccataa ccctgcttcg agcacttcaa tcggcaagcc aagtatttgt cttaataatt
    12721 atctccctct tccttgccat gggacttaat cccacagtcg atgccctgca atcaagaaaa
    12781 ctaaagagat catcagctgt agctgttgtg gttgtagttg ccttggcaat tattgcctta
    12841 tttgccttga ttgtaatacc accagttgcg actcaagcca atgatttaat cgaaagcgcc
    12901 ccacaattac ttgattcact aagaagtaat gccactttaa atcaacttaa tgttgattat
    12961 gggttcattg attcactcga gaagaaattc gaagagtgga tatcagatgg aaagatacta
    13021 actggcgcat ttggaggagt tcttggcgta ggtagaacag ttttatctgg ggctttttca
    13081 gctctaactg tcttagttct cactctctac tttcttacct ctctgccatc agtgaccaaa
    13141 atcttttatc gcctagctcc agcatcaaga agagcacgag tatcaagcat tggcgatgca
    13201 ataatctctc gagttggaag ctttgtggga agtcaggtat taatcgctgg ccttgctgct
    13261 ttatttgttt ttgcactagc ccttggactt gctctgccct atgcggcagc cctatccatg
    13321 gtcattttat ttgttgcttt aattccttta attggtcact ttctgggtgc cagcattgtt
    13381 attttggtcg cccttaccca atctccaagt aaggctcttc ttgctctaat tctttatacc
    13441 gcatatgtgc agattgagaa tttcattatc acaccaagaa ttatgaaacg ctcacttgca
    13501 attccaggct tagtaacaat tgttgcagct cttcttggaa catcacttct tggtttagtt
    13561 ggcggaatcc ttgctgttcc tatcgctgct gcgatattgc taataatgga tgaagtggtc
    13621 tttccaaaaa ctgataattc ttgaattaaa tctcaagcgg tataggcaat cgttcaggag
    13681 aaatgacttt aactatttca ctcaaaactc tatgaacaaa gggctcacct acccataaat
    13741 gtttgccatc tttgatggcg atgagttctg cttgaggaat tggcacaaat cgctgcttag
    13801 cctgctctgg ctttaagtaa tcatcaaact ctggaattag cgcagtaata cctctgccgt
    13861 cttgccccca gaaagcaagg tcttcatcgg ttgaaaatct aagtggcggt gagagaagta
    13921 ttagacccat tactcgttta tctcgcgcat acttcaaagc taaatcactt ccaaaagacc
    13981 atccgctaac ccatagcttc tttattccaa gttgatcgaa agagtaatta attgcagcaa
    14041 ttacatcttc tctctcagta attccctccc caaactgacc ttcacttatt ccttgatcac
    14101 ttttggtgcc acgagtgttg aagcgaatca cattaattcc tgctaggtgt gggaggcgat
    14161 ttgcagcttt tttatagatg tggctatcca tcatcccgcc atgggttgga agaggatgaa
    14221 ggcagagcag gccgattccc tggttatctt ggatgggtct tgcaatttca cctatcaact
    14281 tgagcccatc gctggtgaga aaggtaatag cgctgcgctc ggcgggcaga actgtgctgg
    14341 gtcgtatcaa ctccgccatg cctgtaagtt tatccctgag tatgaaaacc ttcgaatcct
    14401 taaatccagc aactggcgat gtaattggcg ttttttccca aacatctgcc aaagaagttg
    14461 atgagtctgt tgctaaagca agtatcgcaa gtgagcattg ggcttcgtta agttttagaa
    14521 aacgcggaat aatcctgcgt gattgggcat ctttattaac gcgcgagatt gaaagcagcg
    14581 cagatttaat ccatcgcgag acgggaaagc ctttaagtga tgctcgcctt gaagtcagca
    14641 ttgcaataga acatattggc tgggcagcaa aatatgcgcc aaaagtgtta agtgatcaat
    14701 cacgcccctc aggtttattg atgtttaata tggcagcaaa agttcagaga tccccttttg
    14761 gtgtaatcgg aattattgga ccttggaact atccattttt tactcctgtt ggctctattg
    14821 cttatgcctt ggctgctgga aataccgtag tttttaagcc aagtgaattt actccagcag
    14881 ttggtgcatg gcttggagag agttggaaac gtatcgctcc ctttgcagat atcttcacag
    14941 taataacggg tggagctgaa actggagctg cacttacaaa gtcaaaagtg ggcaaaattt
    15001 cctttactgg atcgactaaa actggaaaat tagttgcagc agcttgcgct cttaatatga
    15061 ctccagtgct gcttgaatgt ggtggaaaag atgccgtatt aattgatcgc gatgccgatt
    15121 tgaagaaagc tgctgaagta acgctctggc acgctatggc taatgctggt caatcttgta
    15181 ttgcagccga gcgggtttat gttcatagag atgtagctga gagattttct actttaattg
    15241 tggagcaggc aacaaaaatc aaagcaggtt atgaagatgg cgccaactat ggacctgcca
    15301 ctatgccttc tcaactaaag gtcatcaaag cccatatcga tgatgctgaa aaacgcggcg
    15361 gacgctttct ctttggcgat aagtctgctg taaaaggcaa ttttgttagc cctgtgattt
    15421 tggctgatgt gcctgaggat tcaaaagcaa taagtgatga gacttttggt cctactttaa
    15481 tcatcaatac tgttagaaat atggatgagg cagttgatct agcaaatgct agtaactatg
    15541 gccttggtgc tgctgtctgg tcaaagaaga atggcaagag aatcgcctca ctacttaagt
    15601 gtggaatggt ctcaattaac tcagcttttt caaatactgc agtaagttct ctcccctttg
    15661 gcggtattaa agatagtggg tatggaagaa ctcatggcgc agagggcctt cttgaattca
    15721 cctatccaag agcaataatt aagcctagat ttagaattcc tattaatttc acaacttttg
    15781 atagaagtca gagagctgat cgaattgtca tgttgttaac aaagattttg cataaacgtc
    15841 gcagacgtta aaacctaggg gcgtttctac ttctctcgat ttttggtttt ctcttatctt
    15901 tcttactcca acaagcgcta tgccaatgcc gacgagactc cacatcatgt tcgatccaag
    15961 caacgatgtg tggggttgcc ataggaatta actgatcaca tcctgggcag cgatatggct
    16021 tattagacga tgagccggtg attttgcgga ccatatagat gccttcgcta tgctcttcaa
    16081 tgctttgatg cgagggggaa tcactctctt catcactttt tatcttgcga ccacgctgct
    16141 ttggtctatt tctccgagga ctcacaggga tattctctcg caattaaggc aagattacct
    16201 ccgtgaaatc aaagcttgta aagatattga tcacctgcct tgccgcaact ctgctttcaa
    16261 cagcaactgg ccaagcagat caggtcaaag tcgttgcaaa gaaaaccgct aaacccatcc
    16321 cctctcctgc agctaaatgg cccccagaag gttttaaagg caaagaaggt gtctttgcca
    16381 aagtgccaag tagcaaagaa gtaatcggtt tactctctgc caagaagtca cttcaacctc
    16441 tagttaaaac ttgtgaagag tttgcttgcg gcgcagtttt tgtcgcctct gaaaccggat
    16501 gtaagtggtg ggaggttaat tcctccgtat ttagcctaaa agcctcagat ttaagtaagg
    16561 aaaaaattgg aacactaact acctatcacg ccagcagtaa agaaaaggct caagctacta
    16621 tctttcttat aagtggagct gaggtaactc caggaacttc catcggatca atcaaggtaa
    16681 tttgccatag ggattcatca aataaaccaa aaccaggcaa tatctactca cccattgttg
    16741 ctcccactcc aactgctagc ccaagtaatt cataggaggc agtgatgctt ggtggaataa
    16801 ataacactct ctttttcaca tcttttgctg gctttgcagt aatgggctta tttattattt
    16861 tgcttaaatg ggctttttct cgtggctcat ctttagttga aaaacctaag aagattggtg
    16921 atgaagacga atatggttta ttaaaggttg ttgcctcacc aagtaacttt gtagagggtg
    16981 agatgttgcg gttgaaatta gatgaacatg gaattaaagc aactttgact caaaccaaga
    17041 gtggtccaaa aatactcgtt tttgaaaaag agattagcgt tgctcaagca atcttaagga
    17101 gctagcgatt ctttcctgga acccagagtt gatcgccact ctctctattt tcatagcgag
    17161 caagaacgaa gagcaaatct gataaacgat tcaaatactt tgccgttagt ttatttacac
    17221 cctctccaaa tgaatggatt gcctgccagg ttctgcgctc agctctcctg acaacagttc
    17281 ttgcaacgtg caggtgagcc gaagctggag ttcctgaagg aagaacaaag gacttcaaag
    17341 gagcaaggct tgcgttgtaa tgatcgattt gggcttcaat gtaatcaact tgagattgca
    17401 aaactcgcag cggttcatgc tctgggttat ctactactgg ggtgcagaga tctgctccta
    17461 catcaaataa atcattctga attcgaacta ataattttga gatctcttta tctaaattgg
    17521 cgctagctag cactactccg atataggaat ttgcctcatc aacggtggca tatgcctcaa
    17581 gacgtgggtc atttttagag gttcgactca tatccccaag tgatgtcgtg ccatcatcgc
    17641 cagtttttgt ataaatacga gtcagattta ccatgggctc accctaatag agtcgttacg
    17701 atgtagccac ttaggtaggc aaatacttgg aattgggaag gaattaagag gagtttatgg
    17761 ccaagctcta tcccatagcc gggcagataa accccgatcg cttccgcgtc gttggtggcg
    17821 cgcgattagc tggcgatgtc tatatctctg gagctaagaa ctcagtttta aaattaatgg
    17881 cagtctcact gcttgcgcca ggaagctcaa cgattagaaa tgttccagac attgcagatg
    17941 tctccatgat ggcagagcta ctagagacgc taggttgcaa aatatctaga gattttgaaa
    18001 gtaagagcat cacaattgat gtgccagaag cgccaggtca tcgcgctgag tatgaatcag
    18061 ttagaaaact tcgcgcatca ataaatgttt taggtcccct ggttgcaaga cttggcaaag
    18121 cagaagttgc tcttcctggc ggtgatgcca ttggttcaag aggacttgat tttcatacta
    18181 agggccttgt cgcacttgga gctgaagctc acaatgaaca tggttacatc atggcaagcg
    18241 ctgctaaagg attaactgga actcagattg aacttgaatt tcctagcgtt ggcgctactg
    18301 aaaacattat gagcgcagca gttctagcat caggagtaac tacacttgaa aatgctgcaa
    18361 gagagccaga tattgttgat attggaaact tcttaattgc catgggggca gatattcaag
    18421 gacttggcac ctcaacaatc accatcactg gagttaaatc acttaaaggc gcgactcaca
    18481 ctgcaatttc tgatcgaatt gttactggaa cttgggcatt tgcagcgact atgaccttag
    18541 gtgacatcag cattcatggc gcaaggcctg aacatttaga gctgccttta gaaaaactag
    18601 tttcagcagg ggcggtaatc accagtattt ccgatggaat aagagtgaaa atggatagaa
    18661 ggccaacagc tatcgatgta gcaaccctgc cctaccctgg atttccaact gatctacagc
    18721 cctttgtaat caccttaaat gcaattgccg aaggcgattc cctagtgact gaaaatgtct
    18781 ttgaaggacg tttcatgttt gtcaatgaac tcttgcgcct tggagcaaag attcgagttg
    18841 atggccatca cgcttcaatt accggaatca aagagctatc ttcagctcct gtcgttgcct
    18901 cagatattag agctggagcg ggccttgttc ttgcaggttt agttagtgaa ggaattacct
    18961 ttattgaagg tggccatcat atagatcgtg gctatccaga ttttgcccag caacttcaag
    19021 ggcttggagc agatgtaact agagttgatt agagctttag ccaacgctca cttttgtaat
    19081 aaaacctgac tcaatactta cggtaactcg atttgttcgg taatccttag tcagcataaa
    19141 ttcttcgcca tctttttcgc caatacgata aacccaagag ttagcagctg cgcaaacctc
    19201 cgcttctact tcgctcatgg agatcaactg gtttgctcta ccttctgaga ttaaatcctc
    19261 gttcttcaat gttattggac aagttctggc gctacttcca acttctagat actcgcccct
    19321 ggtttcagtt ctgccaattg ccaagacagc agatgacttt gcctttgcag tagctgtgat
    19381 ggagtaaatt ccagcttcag cgctctggga gattcttgag aggaagaagt aattcttctt
    19441 gccatatggt tcataaaaag gtgaacgctc attaatcttc atcacaattt tctttcccga
    19501 tggcgtagtt atagtgactc ctggaagttg ggagctcttt aacttattag ttggggccgc
    19561 atcaacaatc aagtattcga cgtttaattc atcgccttct ttcaaggcaa aacgaacgct
    19621 tcgctgatct ttagccttag caaagtctgc ataaacagca aatgagatag tgccatcaag
    19681 cagaattggg ctctttgaga cagtgttatg tgaagccttt agattgactg ggtaatgcgc
    19741 aagtgctggc ccagcactca atgtgagcgc aagggcgatt aagaaaaagc tactgcgtcg
    19801 cactctcacc taaaactgaa atacgattat tagcaactga gataaatccg ccacttactt
    19861 caaactcttg cacgctgccg tccactccct caatactgac tttgccatct actaaaactc
    19921 ctagaagtgg tgcgtgatct accaatattc caagatcacc ttctagcgtt ctagcagaga
    19981 gggagatcgc tttgccagag aagactcgct tctctggcgt tacgaactca acggttagcg
    20041 ttttatctga catcttttac ttctttgcca actctgcagc tttacgttcg acatcttcta
    20101 atccgccgca cataaagaag gcctgctctg gaatatggtc atagtctcca tttgctagag
    20161 catcaaatgc tgcaatggtt tcggttagtg gaacaaatga accatcaagt ccggtaaaga
    20221 ccttagctac gaatgtgttt tgagatagga agcgctgaat acgacgagcg cgaccaacga
    20281 gaattcgatc ttcttcagag agttcatcga taccaaggat ggcgatgatg tcttgaagat
    20341 ctttatatct ctgcaagatc tgcttcacgc ggtttgctac tcggaagtga ttctctccaa
    20401 tatagcgagg gtcaagaatg cgcgaagttg aatcaagtgg atccaccgca ggatagatac
    20461 caagttctga aattggacga gatagaactg tggtggcatc aaggtgggtg aatgtggtgt
    20521 gcggcgcagg gtctgtaatg tcatcagctg ggacataaat agcctgcatc gaggtaattg
    20581 agtggcctct agttgaggta atacgctcct gaagcacgcc catttcatca gcaagagttg
    20641 gctgatatcc cacagcagat ggcatacgac caagaagtgt tgaaacctct gatcctgctt
    20701 gagtaaagcg gaagatgtta tcgataaaca ggagcacatc ttgcttctgc acatcacgga
    20761 aatactccgc cattgttaga gctgagagtg caacgcgaag acgcgttcca ggtggttcat
    20821 ccatctgacc aaagactaga gctgtcttat tgataactcc ggtctcagtc atttccaaaa
    20881 atagatcgtt accctcacga gtacgttcac caacaccggc aaatactgaa acgccaccga
    20941 agttttcagc aacgcgatag atcatctctt ggataagaac agttttacca acaccagcgc
    21001 caccaaagag accgatcttt ccacccttaa cataaggagt tagaagatcg ataactttaa
    21061 ttccggttgc aaacatctca gtctttgact caagttgatc aaaggcagga gcagttctat
    21121 gaattggcca acgctctttg atatcaagcg atgaggttgg cacatcaagt gattcaccga
    21181 gggcgttaaa tacatggccc tttgtgacat ctccgactgg aacagagatt gccgctccgg
    21241 tatcacttac ctcaacgcca cgaaccattc catcggttgg ttgcattgag atagcgcgca
    21301 ccaagttatc gccaatgtgt tgggcgacct ctagtgtgag ctttcgcatc tcaccgccaa
    21361 gtgttacctc tacgtttaac gcgttataga tcgcaggcat cgaatctgct ggaaattcga
    21421 tatcaacgac cggtccaatt acgcgcgcta cgcgtccggt tgctgtctta gttgtcatta
    21481 ttcgctccct gcgcttgctg aggcgagcgc gtctgcgccg ccgacgattt cactgatttc
    21541 ttgcgtaatt tcggcctgac gggccgcgtt tgcaagtctg gtcaacgact ttattagctc
    21601 gtcagcatta tctgtggctg acttcatcgc acgtcgtctt gctgcatgtt cggaggctgc
    21661 cgactgcaga agtgcgttat agacgcgcga ctcgatatag cgtggcagaa gggcgttgag
    21721 aacttctgct gcattaggtt caaattcata cattggtaaa agccctgcag gagctggacc
    21781 ttcagcttca acaacttcta gcggaagcat ccgcttattc atcgcaatct gagcaatcat
    21841 tgatttaaat tcggtataaa caatatggag ttcatccaca ccattgatat ctgttgcggc
    21901 atcggcaata aatgctggaa ttaattcatc agcaattgtt ttagcgtgag cataagttgg
    21961 attatcagaa aatccagtcc aagaattctt aattgggcga ttacggaatt tgaagtagtt
    22021 aactgctttt cttcccacca gataggtgtt tacctcaaga ccgcgctcct taagtgtgat
    22081 gactagacca tctgcttcct taattgcact tgatgagtaa gcaccggcca ttcctcgatc
    22141 tgcagtaatg attaatactg cagcgcgctt tggatttgtc gattcggtag ttagcgggtg
    22201 atcagtattt gaaaatgtgg caactgcgct cactgcacgc gttaattcat ttgcgtacgg
    22261 ggtcgaggct gctactcgtt gctgcgcttt gacgatacgc gatgccgaga ttaactccat
    22321 ggcacgggtg atcttcttag tcgctttaac cgagcgaatg cgtcgacgat agatgcgtag
    22381 ttgagcgccc atttatctac ttcttatatt tcgtaatctg ctcggtgcca tcgctgcctt
    22441 ctgcttgagc aggggcctca ttaacagcag atgctgatga agaagcaaat tgctctttga
    22501 aggtattgat agctgcggtc aatttggcaa cagtgtcatc ggttagctct ttgctctcag
    22561 cgataacatc aaagatggcc ttttgttcgc gcccaacata atcaagaaac tctgattcaa
    22621 agcggcggat atcagcaact ggtattgaat ctagctgccc tgtggttcca gcccagatcg
    22681 agacaatcat tctctcgagt gagaatggtg tgtattgctg ctgcttgagt agctccacca
    22741 aacgtgcgcc gcgctctagt tgcgccttag aagctgcatc aagatctgaa ccgaaggcag
    22801 cgaatgcctc tagctcgcga aattgtgcaa ggtcaagacg gaggcgacca gcgattttct
    22861 tcatcgcttt agtctgagcc gaaccaccaa cgcgagaaac tgaaatacca acgttgatag
    22921 cagggcgaac tcctgagtta aagagatcgg tttcaaggaa gcactgtcca tcagtaattg
    22981 aaataacgtt tgttggaatg aaggctgaaa cgtcattacc ttttgtttca ataattggaa
    23041 gaccagtcat tgagccgccg cctaactcat cagagagttt tgcgcagcgc tcaagaagac
    23101 gagagtgtaa gtagaagaca tcgccaggat atgcctcacg acctggtggc cggcgaagca
    23161 agagtgagac tgagcgataa gcctcggctt gcttggagag atcatcaaaa acaattagta
    23221 catgtccccc gccatacatc cagtgctgac caatagcaga gccggtgtaa ggagcgaggt
    23281 atttaaatcc tgctggatca gatgcaggcg ctgcaacaat tgttgtgtac tccatcgctc
    23341 cagcttcttc taacgctcct ttaacagatg cgatagttga acccttttga ccaattgcta
    23401 cataaataca cttaacctgc ttcttcttat caccacttct ccagttatcg cgctggttaa
    23461 taatggtgtc aattgcaacc gcagtctttc cagtctgacg atcgccgata atcaactgac
    23521 gctgaccgcg gccgattgca gtcattgcat caattgcttt aattccagta gctaatggct
    23581 ctttaactgg ctgtctttga acaactgatg gggcttgtag ttcaagtgcg cgagtagttt
    23641 ctgccttgat ttcgcctttg ccatcaattg ggcgaccgag ggcgtccaca actcttccta
    23701 aaaatccatc accaactgga accgagagaa tctctccagt gcggcgaact gttgttccct
    23761 cttcaattcc ggtgaattca cccaagataa caacaccgat ttcgcgcaca tcaaggttta
    23821 gcgcaagtcc taaggtgcca ttttcaaact ttagaagctc attagtcatt gtcgatggaa
    23881 gtccttccac gcgagcaatg ccatcgccag ctaccacgac ggtacctatc tcatcgcgtg
    23941 atgcgctacc tggatcgtat gacttaacaa agtttgctaa ggcatcacga atctcttcag
    24001 gtcgaatcgt taattccgcc atgatttttt ctctccctat actgcgagcg ctctgcccgc
    24061 gtcggccaaa cgattgacga ttgtggcgtc aatcaattca tctgcaaatc ggatggatag
    24121 accacccaaa acttttggat caagttcaac atttaatctc actggctgcc cgatcttgcc
    24181 agagaggctg cttgctaatt tatctttctg cgcctggctt aactcaactg cgcttttaac
    24241 gtgagcaatt actctcatct ttcttgcagc agcggcgctg gcataaaagg cgatggtttt
    24301 ttcaatactt cttccccgca ttccacttac caatgaggca agtagtcttg aagttgaagg
    24361 acataccttt gatgccaata attttgcaac aagaactcgc ttgccctcac ttgaatattt
    24421 tgaagagttg agaatctgac gaagttcgag atcactagcc acgattcttg agaactgaaa
    24481 aatctcctcc tcaagtcgat ctagctcgtt attttgatta gcaactgatg cctcagcttc
    24541 aacggctaat tgctcaatta catcgccaag attcgatggg ctcgaccaac gaagggcggt
    24601 gaggctgatc agcagcttga tgctcagatc cgatgtgctc ttagcaaaga gttcttttgc
    24661 aagttctgct ttggaattag catcccttga gttatcagtt agcgcgcggc gaagtggagt
    24721 tgaggaatca agagttgcaa caacggcaaa caaatgagca gagagcgcag atgcttctgc
    24781 tgcagataag ccctttaaga gctcatcaag tgatttgcga gcagcgataa gtgattggcg
    24841 tgacgcccca cccagtgctg ccattgctct actcattttt acttactctt ctccagatct
    24901 tctaggaaac gatccacaat tcttgactga cggacctggt catcaagtgc ttctccgaca
    24961 atctttgatg ctaactgcgt tgcaagagtt ccgacttcat tacgaagtga agtaagcgct
    25021 gcttgacgct cagactcgat ggcagcttgc gcgcttgcag cgatacgagt tgcctcttct
    25081 tgagctttga ctcgcatctc ttcaatgatt gcagcgcctt gcgctcttgc ttcctctcta
    25141 atacttgcag cttcgccgcg agcatcttgg agttgagctt tatattgctt aagagctgcc
    25201 tctgcttcaa gctgagcctt ctctgctcgc tccataccgc cttgaatcgc atctgttctt
    25261 tctgcaaatg ctttttcaaa acgtggaaca actgcgcgtc gcagcaccat atagagaagt
    25321 gagaaggcga tgaagccaac aattatctct gcggtatgcg gaattaaagg atttaactcc
    25381 tctgccgcca attcaataga gcgcatgctt atttgcttga ctctagagaa cgaatgctag
    25441 aactagaccg aatagcgcta gagcttcagc gagtgcgaag cctaggaatg cgatcggctg
    25501 gagaacgctt cgagcctctg gctgacgtgc tactccattg atatatgcgg cgaaaataag
    25561 tccggttgca atacccggtc caatcgctgc caggccaaaa ccgaccaggt tgagtgtgcc
    25621 ttccatgttt cttacctttc cttcttcttt atccttcaca ataaccgagc gttatcgtga
    25681 gtaaatctgg ttatttaatg ctcatctgct aacgctccgg cgatatatag agctgttagt
    25741 agcgtaaaga tataggcctg caggcattgg accataaatt caaagaatgt cagcgcaatg
    25801 ccgatggcaa aggcaaatgg gctaatcaat ttaagtccaa ttactccctg cagcaagtgc
    25861 tccccgccca agataaatac caaaagtagt aggtgaccag caaacatatt ggcaaagaga
    25921 cgaagactta aagtcagtgg gcggactaaa aagtaagtgg caagctcaag aggggtgatc
    25981 aagatgtagg cgggttttgg aattcctggc aggaacatga tctctttcat atatttgaag
    26041 aaaccttgtt tacggattcc cacataatga aagataaaga atgtaaaagc ggctagaaca
    26101 taaggaaatg agattctaga cattgtagga aattgcagca taggaacaat tccaaatacg
    26161 ttattaacta aaatgaaagt aaagagagta aatagatatg gaacaaagcg cataaactca
    26221 tggccaatta cttcgcgccc taaatcattt cttacaaatg aatagaccga ctctcccgca
    26281 aattggagtt ttgagggaac taatgctgcc tttcttgagg cgagtatgaa gaagattgaa
    26341 atcaaaatta ctgagagaaa aactagaaga attggcttag ttgcaaactc attaccttca
    26401 ataaatggag ggaggttgaa gtctccggca tcggggggat taaatccctc atccttgcca
    26461 acctcggctg cataactaag caagaaagaa ctccttgaag cgttattgga ccttatttca
    26521 tagggaagac ggggtaaccc tatgggcaag aggccaggtt gagaaattca atttagccaa
    26581 aataggggtc aaaagggtta cttgccttac ttaaattcga gggggcttac ttactttcgc
    26641 ccaaagcgga gccagactag ataaagagct gatcccattc ccaaaagcag gccggccaga
    26701 aggaagaagc cggtattaag ccattgatca agcccccagc caatgccgcc ccagattaga
    26761 agccctgaga gcatgtatcc aaagatggtc cagaaggcat tgtcttcgct cggtcttttt
    26821 ggctcattac tgctcatcat cactcctttg attggctctt aggttgaggt aactctagtc
    26881 gtagtttaaa gaaagcccga acctcgccag atagccatgc ggcagagatt gccagagcgc
    26941 ttatcccaaa ggcagaccta tcaagtgagt caggctcact taaacgcgtg accgcaatta
    27001 gaaagagggc aattagcaag agcttggtga aataagacat tagggcaagg gccattactg
    27061 aaattggatc tgagttcctg cttaatattc caacgaggat tgaaacggaa aaaaatatca
    27121 aaactacaaa acttgcaaga gcgctaccaa aaaaacctgg agcgcctaaa taaatagtaa
    27181 aaaagacaat tgcgataact gttgcaacaa gtgatgcgct taaagctccg cgccacatct
    27241 gcttattaga tgatgcaata tccatctatg ccttgacctt cttctctcgc ttagtggtaa
    27301 tcacggcgct tagtagcagt agtgcgccaa ctaaaagcgc tgcccataat ggaataaatg
    27361 ctgagagcat cgcaggaaga gcaagcatgg cggtaactaa gtagagaatt actgcgcttc
    27421 gttgctgcga gtttccccat gccatcaact tatgatggag atgctcttga tcgggagcaa
    27481 agggagaacg ccccgattta actctgcgca gaactgccag cgctagatca agtaatggaa
    27541 tagcaagaac tgcaaatggc aaaagaagtg gaagagcggc aggtccaata ttttctgcaa
    27601 atagcgcgtt agcatcaatt tggcccatca aagtaatagc tgatgctgca agtagtaaac
    27661 ctagaaacat cgatccagaa tcacccataa atatctttgc tggatgggtg ttatgtggca
    27721 gaaaacctag gcagcagcca atgacaattg ccgtgacaag agatggcgcg ccagcccgac
    27781 tgaagccatt ttcaacagcc aacagatatg agaagccaaa gaatgctgcc gcagagatac
    27841 caacaattcc ggtagcaaga ccatctagac catctatgaa gttaactgca ttaataatta
    27901 caacaaccac aactactgtt aatagctgac caatatttgt tggaagaaca atgattccat
    27961 ctattggtag ccagagaatt tgaattccaa aaatcaaaag tattgctgcc gccaggcctt
    28021 gacctgctaa ttttgtaatg gcatctaatt catagcgatc atcgagagcg ccaattaata
    28081 gcacaaaagt tgaagcaaga aatattccta gtagttcttg agagtaagcc ttaccgacta
    28141 aattaaggct acctaccatc actaatgtag ctcccattgc tagccacatc gctaccccac
    28201 cccaacgcgg ggtttctagt gtatgaatat cgcgctctct aactgatgca acagcctttg
    28261 aacgcagcgc aagagagcga atcagaggag tgcacatata agtaagtact gcagcgatta
    28321 aaagagttaa gaggtattcg cgcattaggc tggatagaca ggaaactttg cagttagtgc
    28381 atttacttcc ttacgaatct gagcaatctt tgcctcatct tctccatctt taatcgcgcg
    28441 agcgattaaa gaagcaatca ctggcatctg ctcggtgccc atgccttgcg tagtcactgc
    28501 tggcgttccg actcttattc cacttgttac cgcaggtgat tctggatcat aaggaatggc
    28561 atttttattt agtgaaattc cagctctgcc gcatctttca tctgctactt ttccattaac
    28621 cccagttgag cgaatatcta tgagggctag gtgggtctct gttcccccag atactgctct
    28681 cattccttca tcctctaagg actttgcgag tgctttcgca ttaacaatta cttgcttggc
    28741 ataggcttga tactgcgttg tggcacattc ctttaaggca atagcttttg cagctaccgc
    28801 gctcataatt ggtccgccct gcattcctgg aaatattgct ttatcaatgg cagatgcatg
    28861 agcagcttta ctcaaaatca ttccacctcg aggacctctt aaaactttat gggtggtaaa
    28921 tgaaacaaca tctgcatatt ccactggcga aggaatagct tttccagcaa ctaaaccaat
    28981 gaagtgagct gcatcaaccc acataattgc cccaacttca tcacagatac ttctaactgt
    29041 tttaaaatcg atcaagcttg gataggcagt ggctcctgag cagatcatct ttggttttac
    29101 tcgtagcgcg atatcgcgaa gctcgtcata atcaattaat tcgttatctt gccttactcc
    29161 atatgattca acgttaaacc acttaccaga gaaattaact ttagatccat gagttagatg
    29221 accgccatgt ggaagagaca tagcaagaac ggtatctcca ggctttgtga aagcttgata
    29281 aaccgcaacg ttggcacttg ctcctgagtg tggttgcaaa tttgcatgtt cagccccaaa
    29341 taattcttta gctcttgcaa tgccaattac ttcagcctta tcaacctctt cgcatcctcc
    29401 gtaatagcgc ttccctggat agccttcagc gtatttattt gaaagagttg aaccaagagc
    29461 agcaagaacg gctggagatg tgaaattctc gctggctatt aattgcagat tctggcgctg
    29521 tctatcaagt tcagaaatta atattgcagc aatttctgaa tcttgttcct gtagggcctg
    29581 gaaatctgga ccgaagaaag tctctgacat ggcctaaatc ctaggcggga actgaaatat
    29641 tggcagaaac agagcgcaag tcggcaagtg atattgctcc cgcgcgaaca agccttgccc
    29701 cttcttgctc aatggcaata acggtagagc ttggcccctc tggcagttca cctagatcaa
    29761 agagcgctgc ataatcacta tctaaagctg gaaagaaagt agattttctt ggaacaggtc
    29821 ttccagatag gcaggctgct ccaacagcta gtggacctga ggcaagtgca acgcttcgaa
    29881 caaactctgc atttggcact cgaactgaaa tttcatttaa ggctcttgca tctcccaaat
    29941 cccagactaa accttgagca ggggtgagat taatagttaa aagaccgggc cagaattttt
    30001 ctgcaagcgc agtaattgtt tcaccgaatt ggagagaaat accttttaca gtttttacat
    30061 cgcctactat tacctgcgcc gcagttgctg gtggagattg acgtagttta tgaattttct
    30121 ttactgcgcc atgattgaag gcatctgcta gataaacata tccatgttca agtggggcga
    30181 tgattaactt gccatctcga agagaggcaa ctgctctttc gattaactca ttactatcaa
    30241 ttaagccatc ggttagcgag tatctctctg ccattactct gcctctcgcc tagcgctgat
    30301 ccatcttgga cgattattta aatcgcaatt caaatttatc tcagagtagc cctttgctaa
    30361 ctcagcctca agtaggagag attgactctc gtggtgttcc ataatcagta gaccgccagg
    30421 ctttaataag atcgatgcag ccttaataaa tcttctcgga atctcaagcc catcagctcc
    30481 accaccaaag agcgcctctc ttggttcatt ttcaatgact aactctggca atttatcgcc
    30541 atctgggaca tagggagggt ttgcaataac aatgtcgcac ttaacccctt ctagaacctc
    30601 caaaacatcg ccagagataa cgcgaaccga gacatcgcag gctgcaatgt tgcgcttaag
    30661 ccacttaata gcttcatctg atttttccac agcgacaact gtgactggcc aattcttctg
    30721 ctctgcctca tgggcaacgg agatggcaag tgcgccacta cctgaaccta aatcaacaac
    30781 gcttactggc gttttacttg attggccctg cattgagtga attttttcaa tctcaataag
    30841 tgctgcttca actaacgcct cactctccgg ccttggaatc aagacgccct gtcctacctg
    30901 aaaagttaga tacctaaatg gagcatgccc aattaagtat tgggttggct ttcctgaaat
    30961 tcgccttgta attagatcat caaggagttc gacttgctca agctctaaat caaaatctct
    31021 tgcatgaagt tccatacgtt caacacctag tacaaatgcc aagattaatt cagcatccac
    31081 ctctgaaatt ccagcagatg caaactgcgt ctttttatct tttagaacct gcttgatgct
    31141 cattattttt atgcttcagc agctgagagc ttctctgcct tatcagcctc aagcagcgct
    31201 ccaattactt catcaagctc accatttaat actgcatcaa gattatttgc cttgaaattt
    31261 actcgatgat cgctaatgcg attttctggg aagttatagg tgcgaattcg ctcagatcta
    31321 tcaacagttc gaacttggct ttttcttgtt gcagatgcgg ctgcatctgc tgcctcttga
    31381 gcagcggcca acattctggc tctaagaatg cgaagtgctg attctttatt ctgtaattgg
    31441 ctcttctcat tctggcaaga aactacgatg ccagttggaa tgtgagtgat acgtactgct
    31501 gaatcagtgg tgttaacgct ctgtccgcca ggacctgatg agcgataaac gtcgatgcgt
    31561 agctcgttca tattgatctg cacatcaatt tcctctgcct ctggaagaac tagaactcct
    31621 gcagccgagg tgtggattcg cccttgggat tcagtctctg gaactctttg aactctatga
    31681 actccgcctt caaatttaag ctttgaaaat ggggctgacc ccgcagctgc tccgccgcga
    31741 gcctttactg caacagaaat atccttatag ccacccaaat cactttcagt gacatcgata
    31801 atttccgtct tccagccttg cttctcggca taacgcagat acattctcaa taggtctcca
    31861 gcaaagagag ctgattcatc gccgccagct ccagctttaa tttccaaaat aacatcgcga
    31921 tcatcattgg gatctcttgg taatagcaac tcttccatcg cgcttgcata ttcttcataa
    31981 gctttttcta gcgcaggtat ctctgcagca aaatctggtt ccacttctgc tagttcgcga
    32041 gcagcagtta gatcctcttc agacttttta aaggcgcgat agccagcaat tacaggtcct
    32101 agttgggcat atctttttcc tagttggcga gcttttcctt gatcagcatg aatcgatggg
    32161 tctgccatat ctttttcaag ttgctcatac tctttaagaa tttcaggcat ggtggagaaa
    32221 cgattggcat tcttctcagc catttacgta ctcccttctc ttcaaaatca atctggttcg
    32281 ataaataaga aaagaccgca ccccaattac tcaaaggggc aacggccttt actttaaaag
    32341 ctacttcttg gtctgttgtt tcttagctct ctcttcgaac ttagctacac gtccaccagt
    32401 atcaaggatg cgctgcttgc cagtgtagaa aggatggcta gctgaggaaa tttccacagt
    32461 aactagcgga tactccttac catcgctcca aaccatcttc tggtcacttt tcattgtaga
    32521 gcgagtcaaa attgcaaaat ctgcagaggt atcgcggaat atcacatatc catattcagg
    32581 atgaatatct ttacgcatct tcattgctcc ttatttttca tctagctggg tccgaggaat
    32641 ttcggtgaac cagccactta ggtgcgaagg ctacctaacg cccccacatt tgagccaatt
    32701 ggcccttact cccccttcgg ggattgatca ggaccaacgg tggtcttttg aacctgcatc
    32761 aaaaattcag aattcgactt tgtgcccttc atcttctcaa ggagaagttc aagtgcttgt
    32821 tgggaatcaa gggcgtgtag aactcgacgt agcttccaga caatatttag ctcctcagga
    32881 cccataagaa tttcttcctt acgagttcca gatgcatcga tatcaactgc tgggaagatg
    32941 cgcttgtcag caaatctgcg atccagctta agctccatgt ttccagttcc tttaaactct
    33001 tcaaagataa cttcatccat ctttgagcca gtttcaacta gagctgttgc caaaatagtt
    33061 agtgatccgc catcttcaat atttcttgcc gctccaaaga atttctttgg cggataaaga
    33121 gctgcggaat caacaccgcc ggagagaatt cttcctgatg ctggagctgc gatgttgtaa
    33181 gcacgaccaa gacgagtaat tgaatcaagt aaaacaacaa cgtcatgacc tagctcgacc
    33241 aaacgcttcg cgcgctcaat agcaagttca gcaacggtgg tgtgatcatc tgcagggcga
    33301 tcaaaagttg atgcaattac ttcgcccttt actgatcttt gcatatcagt aacttcctct
    33361 gggcgctcat caactagaac aaccattaag tgacactctg gattattagt tgtaatggca
    33421 ttagcaattg cctgcagcac catagttttt ccagcctttg gtggtgagac aattaatccg
    33481 cgttgacctt taccaatcgg ggcaatcaaa tcaataacgc gagtagtcaa gatatttgga
    33541 agagtttcaa gacgtagacg atcctgcgga tagagtggaa caagttttga gaaatctact
    33601 ctctcctttg aactatctgg ctccatgcca ttgatagtgt cgaggcgaac tagagcattg
    33661 aatttctctc tcttttctcc ttcgcgcgct tggcgtacct gtccggtgac tacatcgcct
    33721 tttctaaggc catatctgcg cacttgttgc agcgatacat aaacatcatt tggaccagga
    33781 agatatccgg cggttcgaag gaatgcatag ttatcgagaa tatctagcaa gccccctact
    33841 ggaagcagta catcatcttc acttaaaact atctcgcgct ctccgcggtt tgagcgatcc
    33901 ctatctctaa agcgatcacg gcctctatcg cggtggcgat ctctgcgcca ctctctatcg
    33961 cctcgcgagt tatctcgtga attattgttg tttgaattat tagagttatt acttgaatta
    34021 tctgaaggca tttcatcctc atcttgatca tcatcactat cggatgatgc tgtctcattt
    34081 gaattgttgt cgcgtcgctt ttgattcctt gcagctcgct ttgcttcgcg ttcagcttta
    34141 gcagcttcgc ggttagcggc ctgcaggtcg gtgattgctg ttactaggtc aggctttttt
    34201 aactctgctg cgccttcgat acctagatta gttgcaattt ttcttaactc aggaagtagc
    34261 aatgcagata gatcgccaga ttctctccga gcacgtgtgc ttgtttctgc catttatggt
    34321 tcttttctag accttaggtg agatctttta gtttgaattt gtgatttttt tatctggtct
    34381 tacgggaagt atcagattgt gggctcaata tgcatgccct acgagataga aatctaccac
    34441 tgcctcgact taaccgacaa atccagggct tggcccgcct tttaaggcta tttataccgc
    34501 tgcaatcctt acgccggttg gagaaatctc aagatccatg gccttgaagt aatcgccccc
    34561 gcttcgaatc aaatcgtcat gctcggcctt agttgcagtg tgtaaaacca gaactgaggg
    34621 ccctgcacca gagatcattg ccgctacccc agcttttcta aatttattga ctagatccat
    34681 tgattttggc atagcctctc tgcgataact ctggtgaaga agatcttgcg ttgcagcaaa
    34741 aagtagctct ggtctggaag ataaagcatg aactaaaagg gctgatcttc cagaattaat
    34801 gctggcatct ttatgagata tctgctcggg aagcatcttg cgcgctttgc ccgttgaaag
    34861 ttgagtatct ggcacaaata aaagagctcg aatatttggg tcaacgctaa agcctgaagc
    34921 acaaccagtt acaacttttg ctctatcttc catccaggaa attgttgcac cgccaaaagt
    34981 tgcagcggca acattatctg gatgtccttc aatatctgta gcaagagcaa ttaggtcatc
    35041 atcgctcatc aaattacttc cagaaagaac taaggatcta gaaagactta ggccgccaac
    35101 aattgcagca gctgatgatc caagtcctct gccatgagga atttcgttca ggcatctaag
    35161 cgctatacct cttggtttgc ctcccataaa ctcaaaacca gccatcattg ctttaattac
    35221 tagatgcttg ccatcttttt tcacatcatc tgcgccctgg cctgttacat caacatcaaa
    35281 tacttcatca tctaaaattt gagcaacgta tgtgtcgcga agttcaaggg ccagcgctag
    35341 gcaatcaaaa ccagggccta aattggcaga gcttgccgga acgcttgcct gcatcggagt
    35401 tgccttataa gtgaggcgat ctttaaatcc tctagacata attagctaag tccaatcact
    35461 ttggcagctt ttttcacatc tactgggaca gtaattggtt tatcggctcc atttaatatc
    35521 cattgaatat cttttaagcc atttccagta acagtaatta caatcttttt gccctttggt
    35581 agtttctttg cagctttata cttaattaac ccagcaagtc ctgctgcact tgatggttca
    35641 acaaagactc cctctttagc tgcaaccaag cgataggcgc ttaatatctc agaatcgctg
    35701 accatatcga tcaaaccttt tgattcatct ctagcttgct cagcctgctt ccaggaagca
    35761 ggatttccaa tacgaattgc agtggctaac gtctctgggt ttttaacaat cttgccacga
    35821 acaatgggag ctgctccctt tgcttgaaat ccccacatag ctggaagtga ttttgaaatt
    35881 cgatcagcgt aatactcttt atatcctttc caataagccg ttatgtttcc ggcatttcca
    35941 actggcagca catgaatatc tggggcaaaa ccaagtgcat ctataacttc aaatgatgcg
    36001 gtcttttgtc cttcaattcg ataaggattt actgaattaa caagtgatac tggatattgc
    36061 tcagataatt gctttgcaag agttaggcaa tcatcaaagt taccctttac ttgtaataac
    36121 ttggcgccat gggcaattgc ttgggcaagc tttcccatcg caatctttcc ttgtggaact
    36181 aaaacgacag gaaccattcc cgctttagtg gcataagcag cagcacttgc actggtattg
    36241 ccggtcgagg cgcagataac tgctttggcg ccatcttcag cagcttttga aatagccatc
    36301 gtcattccgc gatctttgaa cgatccagtt ggatttgcgc cttcaaattt aagccaaaca
    36361 tcatttccta acatctctga aagcacgcag gcgtagatca gaggagtgcc gccttcattc
    36421 aaagtaacaa ttggagtttt actgctaacg ggtagacgct ttttgtactc tgttattacg
    36481 ccgcgccatt gatgtgcgcc agatttctta aataagctca ctatttactt ccttcgatgc
    36541 gaagcacgct ctcaacgctc ttgactacat cgctatctgc aagcgactta acagttttct
    36601 taagcgccga ctctggggcg tagtgagttg caacaattaa ctctgcgcta tcgcctcgac
    36661 ctgtctgtct tactgtttcg atagagacgc tattggcagc aaatagctgg gccacatttg
    36721 ctaaaactcc tggcttatca gccacatcta gtcttattag atactgtgat ttcacgttat
    36781 caattggtgc aatcgccaaa tctgcataat cactctcgcc atggccctgg ccacctctaa
    36841 ttttattgcg agcaattgca attaaatcgc ccaaaatagc actcgctgtt ggctcgcctc
    36901 ccgcgcctct gccataaaac attaactccc cagcagattg ggcttctata aagactgcat
    36961 taaatgctag acgaaccgct gcaagtggat gcctgcgaga aattagtgtg ggatgaactc
    37021 gcactgatat ctcattttct ttggtgagct ctgcaattgc taataactta atcaaacaac
    37081 ccatatctct tgctactgct acatccctag cgctgatctg gctaatgcct tcacagtaaa
    37141 catcacttgc agtaattctt gagtggaaag caagacctgc aagaattgca gcttttgccg
    37201 cagcatcatg gcctgcaata tcagctgtgg gatctgcttc agcaaaacct aaatactgag
    37261 cttcttttaa tacctcttca aaagatgatc cctcttcatc catcttggta agaatgtaat
    37321 tagtggtgcc atttacaatt cccataactc gatttacatg atctcccaca attgattcgc
    37381 gcattgggcg aagtattggg attgcgccgc caaccgatgc ttcatagtaa agatcaaccc
    37441 cagctttatc ggctgcactg aataactctg cgccatgttg ggcaagcagt gctttgttag
    37501 cagtgataat agattttccg ttgctaattg ctttcaatat tagagttcga gccggctcta
    37561 ttccgcccat cacttcaata acaatgtcta ctgatggatc gctaactaca gattcagcat
    37621 cttgagtaag aagttctttt ggaatccgtt cgcgccctgc tttgctatct ctaactgcaa
    37681 tacgagttaa attcaattta gcaccagatc gagcagcaaa atcgccatca tctgccaaga
    37741 gcaagcgagc tacctctgag cccacaacgc cgcagccaag catggcgaca ttaaagctct
    37801 gctgcgcgct aatcattttg acccaccttt accgatctgt cttggttctt cctcaatctc
    37861 caaggctaga aggtcgcgat gagtttcgcg gcgaataatg cttcgcgcct tgccattaag
    37921 gacacaaatc actggcggcc ttgtcatatg gttgtaattg ctagccatac tgcgcccata
    37981 tgcgccggta gcaggaacaa ctagtagatc acccggagcg atatcgctgg gcagctgcac
    38041 atctctaatg atgatgtccc cggtttcgca atgctttcca acgactcgag actttcttag
    38101 tggagcgctg ctatcgcgat ttgccaatgt aattgaatac tgcgcttcat ataaagctgg
    38161 tcgaatgtta tcgctcattc caccatcaac tgatacatac caacgcgttg agccatctgc
    38221 caaagttacc tctttagttg ttccaacttt ataaattgtc accatagcgg ggccaacaat
    38281 tacccgacca ggttcaatag agattcttgg aatctttagc agagcttttt cacactccgt
    38341 cttaactctt cttgcaatca cacttaaaac ttcattagca gaacttggtt tatctccctc
    38401 gatgtatgca attgcatagc cgccgccaag gtctaattct gctagttcgg ctccaaactc
    38461 atctcggtat ttagcaagga ctgcgataag tcttgaagcg cttaattcaa aagaatctgg
    38521 cgtaaatatc tgcgaaccaa tatgagcatg tagtcccctt agctcaagtg aatcatgttt
    38581 cttcacagac aagatcgcat cccaggccgc cccagatgca attgaaaacc caaatttcac
    38641 atcttcatgt ccagtggaaa tagcttcatg ggtgtcagca tcaactccag gatttagacg
    38701 gagtaatact ccttgggtga ctgacttttc atgtgcgatt ctggccacac gatctatttc
    38761 ctgtagtgaa tcaataacaa ttaccccaac cccgtaatca atggctgcag caatttctga
    38821 ctctgacttg ttatttccat gtaattcaat ttttttagct gggaaattgg ctgcaattgc
    38881 taccgctaat tcaccatcag tgcagacatc aagtccaagg ccggcctgtt caatccagcg
    38941 cgcaacttga gttgagataa atgactttgc tgcgtagtaa gcagttccag catttgcgcc
    39001 aaatacttcc tgtagcgctt tagcccagag gcttgctctg gctttaaagt ctgattcatc
    39061 aagaatgaaa gctggcgttt caaactcatc tgctaaatct ctaactgaaa tattgccaat
    39121 agtgattcca ccatttactc gtcttgcact aagtggccaa ataacaggat ttagatcttg
    39181 gctcatatct acatcttctc cggagcgctt acgcccaata gatttaaagc attacttatc
    39241 acctgacgcg ttgcatcaca gagcgatgct ctagatgaat gtattgcact tggtgattca
    39301 tcacctagcg gcaggacgcg acaatcgtta tagaagccgt gatacagagt tgctaactct
    39361 tcaatatacc tagcgatgcg atgaggttga cgaagctctg cagcgcttgc aacaactcta
    39421 ggaaattctg caagagaacc taatagctcg cgctcacgat catgagttag aagatctgct
    39481 tggtagtggt taattccata aggaatagat agatctttag catttcttag cactgcgcaa
    39541 attctggcat gggcatattg cacataaaag acaggattat cgttagtgcg tttcttaagt
    39601 agatcaatat ctaaaaccat aggagtatca actggatagc gaatcaaggt ataacgtgct
    39661 gcatcaacgc caactttctc cacaagctcc tctagagtga taattgttcc cgctctcttt
    39721 gaaagcttaa tctcttggcc accttcaata atcttgacca attggccgat caatacatgg
    39781 atgttgtact ccggatcatc tccagcgcaa gcagccgttg ctttaaggcg gccgacatag
    39841 ccatgatgat ctgctccgag catatagatg cagatatcaa agccccgcct tcttttatct
    39901 agataataag cagtatcaga tgagaagtaa gttaactctc caccagattt aaccaggact
    39961 cgatctttat catcaccaaa atcagttgtc ctaagccaga tggccccttc attttcatag
    40021 gtatgacctt gcgatttaag agttgctaat gctttatcaa tgccatcacc gctatgaagt
    40081 gaacgttctg aaaaccagat atcaaagtgg gttccaaagt tgtctaaaac tcttttctgc
    40141 tgcgctaatt gcaattcata tccagcttcg cggaaagcct caattgcccc cggtccgctg
    40201 agttttttat attctgggtt ctttatgaga atttcttgag ccagctcatc gatgtattca
    40261 ccttgataac catcttcagg tttatcttga ccaagagcgg atgcggcaag tgatgctcca
    40321 aagagatcca tctgattacc acgatcgttg atgtaaaact cacgagctac tttcgctcca
    40381 cctgcgatta aaactcttgc aagggcgtca ccaactgctg cccacctggt atgacccaga
    40441 tgcagaggac cggtgggatt agcacttata aattctagat tgatttttac tccagagaga
    40501 atcttgacct ctccaaactt atcgccttga gcatagatgg tattaactac agcgccctgt
    40561 gaagctttta ctaaagttat atttataaat ccgggcccag caatctcaat tctttcaata
    40621 atctcatttg actttagcga agtagtaata atctctgcga tctgccttgg tggttttcca
    40681 gcctgcttag caagtgcgag cgcaattgaa gttgcataat caccatgatc acgattcttt
    40741 ggtcgctcca aagtaatttc atcaagagcc gcatttgaac tcaactctgc cggcagctca
    40801 ccttttgcaa tagcggcttt gacagattcc aaaatcgcgg cggaaaggag ttcatgaggg
    40861 gcaagctcag gagaatttgg tgaattagag gccgacacct agataaggct accgacccca
    40921 ccttctttct tgcgtcaacg agaaaaaggc cttgattttg acctcaaaaa ggggccttgt
    40981 tatcaaagtg ttatctgctc ccgctcagaa tttgagatat cgactaccgc gcaaggggtt
    41041 agtaaaagta ggtttcaccc caggtgggcc gcccttgcgg ctcatttatt aactactttc
    41101 gaaaggtatc taatagatat gtcaaagcgc cgtctcgcga tctctgcact agttgcatcg
    41161 ttcgcactaa ttctcaccgg ttgctcctca tcagatgaag gttcatctga tggtttcgtt
    41221 ggagtcatcc ttccagatgc tgcatcttca gcacgttggg aaaccgctga ccgcggattc
    41281 ctacaagctg catttgatgc tgctggagta aaagttgata tccagaacgc aaatggtgat
    41341 gctgctgcat tccagacaat tgctgatgga atgttgtctg ctggtgccaa ggttctcatt
    41401 cttgtaaacc ttgactcaga gtcagcaaag gctgtccaag ataaggcagc agaacaaggt
    41461 gtaccaacaa ttgattatga ccgtctaaca cttaacggtt cagcctcata ctacgtttca
    41521 tttgataacg aagctgttgg tcgtctacaa ggtgaaggca tcaaggcttg ccttgatgga
    41581 gcaggaaaga caaaggcacg tcttgtctac ctaaatggtt caccaactga taacaacgca
    41641 accttgttca aggctggtta tgactcagta cttcgcccat taattgatgc aggcgattac
    41701 acactcgttg atgaccaggc tgttccagat tgggataacg tcaagggtgg aacaatattt
    41761 gagcagcaac tatcaaaggc tggcggcaag cttgacgccg tagtctcagc taacgaaggc
    41821 cttggccttg cagctgttgc tgttctaaag aagaacggac tcaatggaaa ggtttgcgta
    41881 tccggacagg atgcaaccgt tgatggtctc cgtgcaattc ttactggtga tctttcaaac
    41941 accgtttaca aggcaattaa ggctgaagca gaagctgctg cagcacttgc tattgctctt
    42001 ctaaatggcg aagaggcaac aacagctacc ggatctgtaa acaacggtgt aaccgatgtt
    42061 ccatcagttc ttctagttcc tgtctcaatc accaaggcaa acgtcaagga tgtaattgct
    42121 gatggcttcc agacacgtga agcagtatgc gaaggcatcg aagacctctg cacagctaac
    42181 ggaatctaag aattcttaga ggaatagtta gctaaaaaga agtaaaagag ttcagcccga
    42241 gtattaaatt actcgggctg aactattaaa ccccactaag gctaggaata aagtggcatc
    42301 accgattctc tcccttaagc gagtaaacaa atcttttggc cccgtccatg tattaaagga
    42361 tgttgatttc tctgcctatg ctggaaaggt aaccgcctta gttggagata acggcgcagg
    42421 aaagagcacg ctaatcaagt gcatcgccgg catctatacc ccagagactg gggagtttat
    42481 ttttgaagga aatccagtca ccatctcagg accaagggat gccacagcac ttggaattga
    42541 aattgtttat caagatctag ctctctgtga caatttagat attgtccata atatgttttt
    42601 aggaagagaa gagataagtg ggattacgct aaatgaatcc agcatggaat ctttggcaaa
    42661 gaaaactcta gatggcttaa gtgttagaac tgttaaatct ataagacaaa aggttgcctc
    42721 acttagcggg ggccagagac aaactgttgc tattgcaaga gctgtgcttt ggaacagcaa
    42781 ggtagtaatt cttgatgaac caactgctgc gcttggagta gctcaaactg aacaagttct
    42841 caaactagta agacaactag ctgataatgg gctggcagtc attgtaatta gccataatct
    42901 caatgatgtt ttccaagtag cagataacat cgctgcaatg tatctcggga gtatggctgc
    42961 gcaagtagaa aagaactcag taactcaaaa tgatgtagtg cgtctcatta ctactggagc
    43021 ccaaaaatga gccaggattt aaatatcaaa caatcagcta acgattggtg gagcaaggtc
    43081 aaagccggcc aagttggagc acttccagcc ctgcttggac tatttgttct ctgcatcgta
    43141 tttggcactc agagttcggt atttttaacg ccaggaaatc tagccaattt acttacccag
    43201 gcagcggcag taattgtcat tgcgatgggc ctagttttcg tgctcttact tggagaaatt
    43261 gatctctctg ctggttatgc cgcaggagtt tgcggcgccc tcttggtaat catgattact
    43321 gagatggatt atccctggta tctggcattt ccaatcagcg ttcttgctgg aattctcctc
    43381 ggaggcgtta ttggttattt agttgcccaa ttaaagattc cttcattcgt ggtaactctt
    43441 gcaacattcc tcgctttcca agggctatta cttctcttgg ttggcgaggg cggaacaatt
    43501 cgtattgaag atccagtaat tctggcagtt gagaacaaga acctctcagt ttcctatagc
    43561 tggattttct tccttctcac cgccggcgct tatctcctct ctggcttact gaaatttaga
    43621 aagcgcaaaa aagctggtct agttgataat ttatttaaac tctgggtaat aaagactttt
    43681 gttttaattg ctattggaac tctggccacc tgggccttaa ctgtagagcg cagcaataat
    43741 ccagatttgg taagtcttcg aggaattcct tatgtagttc cggtaattct ggttctttta
    43801 gttggtgcta ccttcgtgct ctctcgcacc tcatttggcc ttcatatcta tgcagttggt
    43861 ggaaatgctg aagctgctcg cagagctggt attaatgtca agatggtaag aattacagca
    43921 tttatgatct gtactggttt tgcagcagtt gcaggaatga tctttgtatc aagagcaaat
    43981 tccatctcac caacaactgg tggaagttca acgcttcttt atgcagtagg cgctgcagtt
    44041 attggcggaa caagtctttt tggtggaaaa ggaaaagtta gtgatgcaat cctcggcggt
    44101 atcgttgtag cagttattga taatggtatg gcgctacttg gctatccagc tggaattaag
    44161 ttcatggtta ctggcgcagt tcttctaatc tcggcaacta ttgatgctgt atctaggcga
    44221 ggagcgagca cgagctaaag gctaagtatt taagtacttt ccagcggttt ttgactctgg
    44281 caattactgg gtatccttac gcctccaact cttgcgggag tggtgaaatg gcagacacgc
    44341 aggtcttagg aacctgtgct tcggcgtgtg ggttcaagtc ccacctcccg caccagtagt
    44401 gcttctggat acataaaaag aaaagaaatt aaattctaaa taattctttc tcagatcttt
    44461 gctgctgcaa aacctttctc aagatcagca ataatgtcgg agatgtcttc aattccaatg
    44521 cttagtcgta ccatccctgg cgtaattctt gcgctgcgca gcccatcttc gccaagttgg
    44581 gaatgcgtag tagaggcagg atgaataact agagagcgaa catcaccaat atttgccaca
    44641 tggctaaaga gcgaaagccc ctcaacaaat ttcttaccag cttctagtcc gcccttaata
    44701 tcaaaagcca taactgctcc agcgcctttg gctgcatact tcaatgcatt tgaatgccaa
    44761 gaagaacttg gcagacctga gtagctaact gactccactt gattgtgttt tgccagccac
    44821 tgggcaactg ctagggcatt ttgtaagtgg cgatccattc tcatacttaa cgtctctaaa
    44881 ccttgcgcca aaagccaggc attaaatggc gaaacgcttg gtcctaaatc tctcagcagt
    44941 tgcagtctgc atttaaagat ataggcgaca tttccaaatg atgatcccac tcctagatct
    45001 tttgcataaa ccatgttgtt atagctgcca tctggttgat taaagccagg aaatttctct
    45061 ggattcttgg aaaaatcaaa atttcctgaa tcaactatcg cccctaccat cgcgctgcca
    45121 tggccagaga gaaatttagt cgctgaatga gtaacgatat cggctccata atcaaagggg
    45181 cgggtgacat agggcgtagc aatagtgtta tcaacaatcc aaggcacttg cgcttcatgg
    45241 gcaacgttgg cgattttttc aatatcaaga gctacgccta atggattaga aattccttca
    45301 ccaaagaaag ccttggtatt tggctttacc gctcgcttcc aagaatccat atcgctagca
    45361 tcttcaacga aggtaaattg aataccgaat tttggtagcg tgtatttaaa tagattgtat
    45421 gtcccaccat aaacataagg gcttgccaca acgtgatcgc cggcttcggc aacattcata
    45481 attgcaagag cggtagctgc cattccagat gaaactagca gtgatgcgat tcctttctct
    45541 aaggttgcaa gtctcttctc tactgcatct tgagttggat tattaattcg ggtgtaaaca
    45601 tttccagctt ctgccacccc aaatagattt gcagcatggg cagtgtcgcg aaattggtaa
    45661 gcagtagttt gatagatagg aagggagcga gatccggtag ttggatctgg tgtttgtcct
    45721 gagtgaattt gttgggtatc aaacgaccag tcttgattct ctgacatagc taacctcttc
    45781 gttgaagttt tcgccgaatt caaccctcag cggcacattc tgtgcttttg ctctcagaat
    45841 agtagaaatt tacctaaatg tatgcacagc gccatagaaa tctgtatttt ccttaatcgc
    45901 tttttattca agacagcgtt agattagcgc catgacaaag gcgtcatcgc aggattcttc
    45961 acgcgcaaaa tcgccatctc tagaatctcc cctagcaaca gcgctctcac aattaagaag
    46021 agctattgat caattaaaac tctctgaaaa tgattggaat accctttcaa ctccgcgccg
    46081 catattggaa gtagctgtcc ccttgcgccg cgatgatggt cgtgttgaga tgtataaggg
    46141 ctatcgagtt caatattcaa caacccgcgg gccttctaag ggcggcgtta gattccatca
    46201 agatatcgat ctagatgaaa cagttgcact tgcaatgctt atgacctgga agtgcgcact
    46261 taccaatctt ccttacggcg gagctaaggg cggagttgca gtaaatccta aaactctctc
    46321 acttgctgaa aatgaaagat taaccaggcg ctacacctca gaaattcttc caatcattgg
    46381 tccagagcgc gatattccag cccccgatgt gggaactgat gaacgcaata tggcttggat
    46441 gatggataca tattcagtga atgctggatt ctctgtccct ggagttgtta ctggaaaacc
    46501 aattgttctt ggcggatctc ttggaagaac atcagccact ggagatggcg tagcaatttc
    46561 agttcgcgag gcgctaaaac ttaaaggaat tgatccaaag ggtgcaacgg ttgctattca
    46621 aggttttgga aaagttggat attgggcggc ccttgcctta gaagaaatgg gactaaaggt
    46681 tgttgcagtc agtgatgtct caggtggtgt tactggattt aaaagcgtta ctgatttgca
    46741 caagtatgtg caagaaaatg gcactctctc aaatgctcct ggaacagatc gcctgactaa
    46801 tactgagcta cttcatctcg gggttgatgt tttagttcct gccgctctat ctgatgcgat
    46861 cactgaagca actgctagcg gaattaaagc caagattgtt gttgaaggag ccaacgctcc
    46921 aacaactccc tctggcgatg caataatgaa cgagaacgga gtgctagttg ttccagatat
    46981 tttggcaaac tcaggtggtg taatcgtctc ttactttgaa tgggttcaag ataaacaaaa
    47041 ttatttctgg agcgctgagg aagttaagac caacctaagc accatcttga tgcgagctat
    47101 cactgaagtg gccgataagg ccaaggatga gaagctcact tggcgcgaag cggccaatat
    47161 gatcggtgtg gcccgcgtag cccaggccca tcgcctccgc ggcctctacc cataatcaag
    47221 cgcttcaccc cattttcaag ctagagccag tcgtgggaag attcacgccc cccgaaagta
    47281 cttaccagaa gtgctgaact aatgaggagc tgaataaagt ggcagataag aattttttat
    47341 catgggttgg atttaagggc gaagaaagtg atgccagtaa aacaaatctt gccaccggat
    47401 cagcctcgct agaacgcatc cgcgaacttg aatcccagtt gaacgatctt cgttcgcgcc
    47461 gcgacatcac agggctaacc aaggaagagt ttgaaattct ggccaccgag actgcgatgt
    47521 ctattattcg cactgctcaa cagcgcgaag ctaaagcaaa ctcaagtgct gagaaagttt
    47581 taaacgagag taagaaacta gcaagttcga ctttagaaca agctgaagca aaagctaaat
    47641 cacttctatc tcaggctgag tcgcgcggtc gtaaatacat tgaagcagca gagagtgatg
    47701 cttcagatat catttcaaaa gcaacttctg aagctgaaga tcttcttgcc agcaagaagc
    47761 gcgaaggtgg cgcaatcaca gctaatgcca aaagagaggc agagcgtcta attcttgatg
    47821 ccagcactga agtttcagag tatagaaact ggcttgctgg agttatttca gaggcagagc
    47881 gcttataccg aattcagagt caatcacttg attcagccga aaatgcgatt tctcaatcta
    47941 gacagagact tgagagcgca tttaatcgac tagcagagct acaaaagcag gttgtctcag
    48001 caatttctgc agatggcaca ccaagtgcgg atgccaaggt ggctgcaaag atcagcgctg
    48061 caaaaaatgg tgaagccaag agtgcagcaa agaaagctcc aagtaagaag agcgcaacta
    48121 agaaacgtcg gtgatgtctt ctagcgctcg ctatatccac tcagttgatg gccttcgcgc
    48181 cgttgcagtt ctagcagttc tgctctatca cctaggcatt gattggattc cagggggttt
    48241 tcttggagtt gatctcttct ttgtaatctc aggttatgta attactggac tcattcttga
    48301 ctcgatcgaa agatcaggca ctcttgatct aagagccttt tatttaagtc gcataagaag
    48361 attacttcca gctctagttg ccatgattat cttcaccctt ctctttatcg gagtttatgc
    48421 accagagaca gttagaagat tcgtctctga tattggatat gtcttatcag gatctatgaa
    48481 ctgggcccta gttgcaaggc agcaagatta ttttgaaagc attggccgcc ctcctttatt
    48541 gcagcacacc tggtcgctag cagttgaagc acagttttat ctgatctggc cgctagttct
    48601 tctttttatc ttgaagtact tcgggaaaaa gagagtttca attgtcgctc ttgccatcgc
    48661 cattgcatca ggggttgctc tctttgccta ctcagtgcag attgacactc aagcatcagc
    48721 aattagccat gtctactttg gcactgatac tcactccatt ggattatttc ttggatctgc
    48781 attagctgtc agttggaaac cagcaaatct caccagagag ataagcaaga gagctcaaga
    48841 cttcattgat ctaattgggg tagttggttt gcttggacta cttggaacat ttctcttcat
    48901 caatgaaagt gatccaacgc tctatcgaat cgcattcccg ctatctgctc tatttggatg
    48961 tgccacgcta atctcagttg ttcatcctgc atctagattt gcaccaatac tgagcactaa
    49021 ggcgctgatt tggattggag agagatccta cggaatctat ctctggcact ggattgtctt
    49081 tcagttaacc aggccatcac ttgatttagt cggagatgat tgggctctct atgccttaag
    49141 agtattgatt gtatttgccc ttgccgatat ctcacttcgc tacatcgaaa taccagtaag
    49201 acgtggatat ttcgaactat ggttccgcgg catgaaatat cgcaccaagg cagtgcgaat
    49261 cagacagaaa ctctcaacga tattaacggt agttgcttta cttggcgcaa cgactctggt
    49321 tgcaaatgat ggaataagaa aagctgatga aattgcagcg caagagaaat tagcagctga
    49381 ggtttcagaa cctgttgatc aagtaatcac caaccctgat agccctgggc tctgggtcac
    49441 aggagattcg gtaattcttg gcatcagaaa tgttctagct caatttgagc gaattgaatt
    49501 aataaatgcc agagttggca gacaaattgg tgagctgatt gaagttgcaa gaacggatca
    49561 gcaattcgtt ggagcctccc cagtggtact aaatcttgga aacaataatc gcttggtcga
    49621 acgcgatgtt gtggagctca tggaagtagt taagaatcag ccaaaaatca ttgtagtaaa
    49681 caccgctgtt ccaagatctt ggaagggtga aaataacgca ctaataaaga gtgtcacctc
    49741 gcgatatccc aatacaatct tggttgattg ggattccata tcagcaaatc atccagagtt
    49801 ctttgcctcc gatggagttc atctaaatcc gccaggagtt aatgcttatg tctctgcaat
    49861 tcgcgaagtt ttacagaaat aaaaagaccg cctctctttt ttttaaagag gcggtccctt
    49921 ttagtaattt aattagttct gatacttgcc ctttagtgag aacttagcag catcaacttt
    49981 tgtgccatct gcatagacag attgcagcag gaactgagtt ccgccttcat cacttgcaag
    50041 tgcgatatca aatgagagct gatctgctcc aactgttgaa acttcaacaa agtcaacggc
    50101 gttataggag acagatacgg cataaccagt taatccatca cttgcagcag gtgccttcca
    50161 gcgaagtgtt actcccccag atgcatttct aattgcaaca aagccagtag gagcttcata
    50221 agctgtaatt gcaggctctt cactttcact ctctgatggt tcagcacttg gctcctcaga
    50281 aactgtctct gttggagttg gcattgcgtc atcatcatca cttgatgagt tagtgagtgc
    50341 aaaaattgct agaaggacaa ctcctgcaat aactagaaat agaacgctat ttgatttctt
    50401 agccggagct tgcgccgctg gtgcgctcgc gctctttgct ggagccggtg ttgatgaaac
    50461 tgaagttgca cctcttgttg gaacaggtgg aattacaatc tttgaaagat catttcttga
    50521 gcttgctttc ttcgcgcttt tctttgcagg gcgcttagct gcggctttct taggcgcacg
    50581 cttacttgcc ttcttaacaa ctctcttcgc agctttctta gctgggcgct ttgcaggggc
    50641 tttcttaacg cgccgctttg cagaagcttt cttctttgcc gccactatta ctccttcgct
    50701 ctaaaacttt gggcacttac tcttagtaat ctaactagga gcgcgctttt ctattccaag
    50761 cctacctgcg agtgtgcact ttcaacggtt atgaaaggga gagaaaaggt gaaatttttg
    50821 gggctatttg cgctaaagct ttgcctctat ggctaattga atccttcatt tcagggctca
    50881 tctgcgcagt tgtgatgtca aagcccgttg gttgaaatat tgggtcatag ccaaaacctt
    50941 gatctcctct ggggctatca attatctgac ccaagagctc acccctagaa ataatgctct
    51001 gcccatcggg cctggtaagt gccaatactg caataaatga tgctgatcgc ttagttatcc
    51061 ccgccatcag ctttaagact ttggcattgt ttgctgcatc atcaccatgt actccgctat
    51121 agcgagccga gtagatacca ggttcgccgt taaggctatc tattgctagc ccgctgtcat
    51181 cagctaaggc agcaagacct gagatctggg taactgtgcg cgctttaaga agagcgttct
    51241 cttcaaaact atttcccgtc tcgtcaatat cgccgatatc aagatctgcc atagagataa
    51301 gtgaaatttc tggagcgtga atttttagca tccgctcaac ctctaaaact ttgcctttat
    51361 ttctagttgc tagtactaaa gtctttttct ctttcgacat taattttcta tcttgttagc
    51421 gcttgcgcct gaagcgatgc gatctgctta cagccatttg ctccaagatc taacagctta
    51481 tcaagcagag ctctatcaaa gggctctccc tctgctgttc cttgaacttc aatatatttt
    51541 ccctcaccag tgcagacaat attcatatca gtctgggctc tgacatcttc ttcatagcag
    51601 agatcaatca ttggctcacc atcaataatt cccacgctaa ctgccgcaac tgagccgatg
    51661 agtggcgatt tgcctgcgat atgtccggct ttcatcgccc aagaaattgc atcacttaac
    51721 gcaacatatg ccccagtaat cgctgctgtt ctagttccgc catctgcttg aagcacatca
    51781 caatctaaaa caattgtgtt ctcacctaaa gctttgaaat ccacaattgc tcgaagtgat
    51841 ctaccgatta atcttgagat ctcttgagtt ctaccgccca attttccttt aacgctctcg
    51901 cgatctgagc ggctatgtgt ggctcttggc aacatcgcat attcgctagt cacccagcct
    51961 tttccacttc ccactaacca acgtggaact cctggggtaa atgaggcgac gcaaagaact
    52021 cgagtattac caaatgaaac tagtacagag ccttccgcat tattgagcca attacgctca
    52081 aaggtaattg gtcgaagttg ctcagggctg cgcttatcgc tgcgttttac ctcagacact
    52141 taaatactct ccattcattt aaattaaatc tgacccttaa caaagttaac ttcagggcca
    52201 agaaagcgcc gtgctagatg ggcaaaggta tcggtgtcgc cacttgaaat gaattgatga
    52261 cttggcgcgc tcttctcaac tcttagaagt gaattttcta ctaagacttt gtagagctct
    52321 tttgcggtct cctccgcact ggatactaag ttgacctcac tacccatcac ataggagatg
    52381 actccagtaa ggagcggata atgggtgcaa cctaaaacca aggtatcaac cccagctgcc
    52441 tttagcgggg ctagatattc ctgggcaacc ttggttacct catctcctga ggtcattccg
    52501 ctttcaacga atgagacaaa ttttggacag gcaagtgctg tcagctcaag gtgtggggct
    52561 gcagcaaagg catcgttata agccttggaa tctattgtgg tttgggttcc aataactcca
    52621 accttgccgc tcttgctggc agcaacagct cttcgaactg caggttgaat cacttcaata
    52681 acaggaattg agtagcgctc cctggcatct cttaacgtcg cagcacttgc agtattacac
    52741 gcgataacaa ttgccttaac cccggcatta actagcgagt ccataatttc tagggcgaag
    52801 gagcgaacct cagcaagggg ccttggtcca taggggccgc gagcggtatc gccaaaataa
    52861 atcagggcct catttggcag ttgatcgatt actgctctag ccactgttag gccgccaagg
    52921 ccagaatcaa agatgccaat tggagctgaa ctatccagat tggccatagc ggtaagtcta
    52981 aaggtgctag ctgcgatggt tgatcctttg gatttaactg aaaagctctt cacgctcaaa
    53041 gataaatgtc cgatttgccc taataaaaga tcagaaaaag ataacatttc ttaagaattg
    53101 ccgaggcgtt ggcttgaatt accggtgagt agagagcacc ctttcatcta gtcgcaccac
    53161 ccgaggcttc atatgggcct acggaaggcg cgatcgcttg gccctaattg agtcgagcaa
    53221 acctaattgc ccacggaggc ttatcgaatg caactagacg catctcagtg gagcacggtc
    53281 tacaacatcc tctcctttgg acttgtatcc atgctcgcaa caaccgtcta taccttggtt
    53341 tcacagaacc gcgttctacc taagtatcgc aacgcgcttg ttctctcatc aatggttacc
    53401 ttcattgctg cttatcacta cttaagaatc tttgattctt tcaaggatgc aacaaaagaa
    53461 ggcacctttg aagttgtaac cgattacaac gtaagttcaa tggcattcaa cgagggatac
    53521 cgctatgttg actggatcct taccgtgcca ctacttcttg ttgaggtaat cgcagtatta
    53581 gctcttgcaa aagaagcagc tcgctcacta atcacacgcc tcgttcctgc atcagcagca
    53641 atgatcgctc ttggttatcc aggagaagtt tctgcagacc agggtgagaa gattatgtgg
    53701 ggcgttctct ccacaattcc tttcctctac atcctttatg ttctattcgt agagcttggt
    53761 aagtccctag atcgtcagcc tgaaggagtt gcagccactg ttggtcgcct ccgccttcta
    53821 ctgattgcta catggggcgt atatccaatc gcctacctcc taccaatcat cgatgcagat
    53881 ggagcagcaa gctctggagc attcgttgga cgtcaagttg gttacaccat cgctgacgta
    53941 gcagctaagt gcgtattcgg tctaacaatt cttaagatcg cacgcatgaa gtcaattgct
    54001 gaaggaatga aggacgatca ctaagaagta aagaagtagg aatcagggct cgggagaccg
    54061 ggccctgatt ttttttattc tggagagact tcttccaata agccttgttg cagccagccg
    54121 atccaggaat aaactgcgta gacagctttc attggatcgc tctcaagcat gagctcatat
    54181 ttattatgaa tagccttggt atcaatatct gcgctctctt gccggttgcc accaattcca
    54241 agtcgcaccg caagtgcaag ccttaaatca ttaagccctg agagccaatc aagtagctct
    54301 gcgctattga taatcagctt aaaagagcct ttatcaattg gagtattgcc gccggtaatc
    54361 tcctcgggct ctccttggct agttagagct tcataaatga ttcgagcatg agctttcttc
    54421 ttttcgcgaa gcccatattc ggtatagcga cgaaattcac tagaggaggc tgaatcttca
    54481 taagcatcag gaaatagcct gcgcagaact ggatcatcag gaagtgcatc agaatcagat
    54541 attcctacga tgtgcattag tggatcttgc gcctcagcaa gagagtcgcc ttgagagaga
    54601 agctctagaa gttgctcggt caaactaatt aatacatgag attcttgagt tgaaatctcg
    54661 attgagatct cgccattttc ttcccgttta aatgatgaca tttaattaat actcattatc
    54721 tcgttgtagc gttgcccaaa ggccatattc atgaagcctt tgcacatctc gctccatctc
    54781 ctcgcgagta ccacttgaaa cttgagctct tccttcatta tgaactttta acatcaactc
    54841 agttgctttc tcttttgagt aaccaaagag caccatgaag acataagtaa cataggtcat
    54901 taaattaact ggatcatccc aaactatgca gacccatggt cgatctgctt ttaaattaac
    54961 ttcgctatct acttgcgtag cggtgcctag cgccattact tacctaaagt tcgttattgg
    55021 attgatcatc tggccctttg cgaaggccgg catgaatctc tttacatgtt ggacagattg
    55081 gaaacttatt tggatcacga gagggaatcc atcgcttgcc acagagcgct gttactggtt
    55141 ttccagatac agctgattca actatttttt cttttctaac ataatgtgag aatctttcgt
    55201 gatctccaga atccagctgc tcttgagtgc gagtatcaat caaggtatcg ccatcgctac
    55261 tcggttttcc tatgccaaat agccccatca cctgctttag ctcaaaagct tgcttcatag
    55321 cgctaataat agggcgagta ggtagagctc caggtaataa ctctggggcc gtaagatatg
    55381 cagatgaaag atctcttgcg cagccaagat ctgctcaaat ctgatgtgga gatgcttctt
    55441 gctaccgcat ccgagtttgc aagtgagcca cttaaggcat caaatcttct cgccaacaaa
    55501 actgttgcaa tctatatgtc taagccatca actagaactc gtctggcatc tgagagcgct
    55561 gtagctcatc ttggtggcac cccaatcttt cttcgcggcg atgatctcca aattggtcga
    55621 ggagagacaa ttgcagatac tgccaaggta ataagccagt ttgcccaagc actaataatt
    55681 cgcaccttcg ctcagagcga tgtcgatgaa ttaggtgcta atgccagcat tccggtaatt
    55741 aatggcctta ccgattatga ccatcccacc caagcacttg ctgattggct aactatccgt
    55801 gaagtatttg gaaaagatat ttctggaaga aaatttgcat atttcggtga tggaaataat
    55861 gtcactaccg cttggttaat gatgggtgcg attatgggcg ctcatgtggt tgcggcaact
    55921 cctgagggaa agtttgctcc taaaaaagaa attgtcgagc ttgcaagtaa gttagcttta
    55981 gaaagcggcg gaaaagtaga agtaacaagt gatgccaagt ctgccgcgag cggggccagt
    56041 gttttatata ccgatgtctg gatgtcgatg ggagatcctg aagctgaccg gattgagaaa
    56101 gagaaaaccc tttcaagtta ttcagtaagt gatgagttga tgggccttgc tagtaaagat
    56161 gcaatcttta tgcactgctt gccagctcat cgcggccaag aggtcagcgc atctgtcatg
    56221 gatggtcctg cttcaaagat ctggcgtcag gcttttcatc gccgaactac cattcaagcc
    56281 attctctatc actgcctgcg tggcgaatta aagggaagga attgaatcta atttcattat
    56341 cggttcgccc atttgaagtg gccagctttt tagccaaaga gataggcttt tgatatgcgc
    56401 gtagtggttc ctgctgaagg taaggctggc gaaaagcgag tagcactcct tccagacatc
    56461 atctctaaat taactaaagc agggcttagc gtctccattg aatcacgggc cggtatacat
    56521 gctggggcaa gtgatgagag ctatatagag caaggcgcaa caatttttgg cgcaagcgat
    56581 cagagaaaag aattagctgg cgctgatgtg atcctctcgg ttcagccact aacgccagat
    56641 caggcaagta atctaaaaag tggcgcaatt actatttcat ttctctcaag tgttagcgcc
    56701 aaagagagca ttgatgcact tgctaaatct ggagcaacag ccttttcact agaggcgcta
    56761 cctagaattt caagagcgca atcgatggat gcgctaactt cccaggcgct ctgcgctggc
    56821 tataaagcag cgcttgtggc agccgagctt tctccgcgct ttttcccatt acttatgact
    56881 gcggccggaa ctgttactcc tgcaaatgtt ttagttcttg gcgcaggtgt tgcagggctt
    56941 caagcaattg caacttctag aaggctagga gcagttgtct ctgcctatga tgttcgccca
    57001 tcttctgctg atgaagttaa atcgatgggc gctactttta ttgatttaga gttagaagca
    57061 cttgaaggct ctggaggata cgccagagag atgagcgagg agcgcgcagc aaaacaacga
    57121 gaacttctca ctccctacat cacaaaatct catgttttga ttactacagc agcagtgcca
    57181 ggaagagctg ctccgatgct agtaactaag cagatgtatc aagcaatggc tcctggttca
    57241 gtaattgttg atctagctgc tgaaagcggt ggaaatgttg aaggatcaaa accgggtgaa
    57301 gtaattactg ttggaaattc agttttactc tggggcggaa aagatgttcc aagccaattg
    57361 ccatttcact cttcctactt gctctcaaga aatatggtca accttctact cttgatgatt
    57421 aaagatggcg caattgctct ggatttctct gatgaaatta ttgacgcctc agcccttact
    57481 catggcggaa atctaaggaa ggggtcctaa agcaatggaa gcaatggcga taatttccat
    57541 tttcacgctc tccatatttg ttggatttga ggtagtttcc aaggtctcat ccactcttca
    57601 caccccactt atgagcggtg ctaatgcaat tcacggcgtt atcttggttg gcgcaatcat
    57661 tgtggccgac catagcgata actcgctaga acttggttta tcactggctg caattgttct
    57721 tgccaccatc aatatggttg gtggatttgt cgtcacagat cgcatgcttg agatgtttaa
    57781 aggcaaaggc gcaaagagta aaggctctgg tgataagtaa atgagccgct ttttatatga
    57841 attaatcggt cttagcgctg gcgttatgtt cattctggca cttaaggggc tatcgcatcc
    57901 aagaactgca aggcgaggaa atcttctagg agcagcggga gcaacaattg caaccgttgc
    57961 agtctttttc tatagcagcg atggcgagct acccttaaat aatcttggct ggatcttagg
    58021 agcaattgca tttggtttaa ttattggagt tcctgcagca agaagagttc agatgacggc
    58081 gatgccgcaa ttagttgcgc tctttaatgg cgttggcggc ggagcagcag cccttgttgc
    58141 aattgttgaa tatctaaagc taggccagag cgcatcaact acagttgtta tcgcaacagt
    58201 ctttacagta atcgttggct caacttcatt ttctggatca attgtcacct ttctcaagct
    58261 tcaagagttg atgaccactc gccctgtggt ttttgctggg ggaagatttg ttatcgctgg
    58321 aactcttctt gccacacttg gatgcgctgg ctgggttgtc tcatcacttg gcacaacccc
    58381 actactagtt cttgcaggtc tttcacttgc ctttggtatt ttatttgtgc ttccagttgg
    58441 cggcgccgat gttcctattg ttatctcatt actaaatgct ttcactggtt taactgttgc
    58501 cgctggcggc tatgtccttg actcaacgct tctaattatt gctggaactc tagttggcgc
    58561 ctcgggaaca attcttactc gcttgatggc agaggctatg gggcgctcac tctttggaac
    58621 tctgtttggg gcatttactg caaaacctca agatagttct ggcgctgggg aagatcggcc
    58681 cgttaaatca ggatctcctg atgatgtggc tattttgctt aactatgcgc gcagagttgt
    58741 aatcgttcca ggctttggtc tggcagttgc ccaagctcaa cacacagtca gagagcttgc
    58801 agacctctta tctgccaagg gaattgatgt ggcttatgga attcacccgg ttgcaggtcg
    58861 catgccgggt catatgaatg ttcttctggc tgaggccaat gtgccatatg agcagttatc
    58921 tgagatggat gaagttaatc caacatttcc acaaactgat gttgcactaa ttattggagc
    58981 aaatgatgtg gttaatcctg ctgcaaaaac aactccagga tgtccaatct atggcatgcc
    59041 aattcttgat gtagcgcaag caggaaatgt aattttccta aagagatcta tgagaccagg
    59101 ctttgctgga attgagaatg aacttctctt tgactctaaa actacgcttc tctttggcga
    59161 tgccaaggcc tcgctaacaa aagttgtgag cgcacttaaa gccctctaaa acaattaggt
    59221 aggagtaggt ctactatcta gccatggagt cattagctct tctagtttca attattgtct
    59281 ttccagcaat gtttggtgga ccgattgcgc tcttgctatc actttatcga ccagcgggta
    59341 tgtcgaactt tcgcagaatc ttcattattt tgtttgcaat tctctcaatc atcgttggaa
    59401 tttttctgct tattgaaaat gtcagcagag gagcaaatgt aattggagca cttggtttaa
    59461 gcactggcct tggatcgctc tggcaattga agagatatag aaaacaaact ccttaaaatt
    59521 tctttaagga gtttctctgg tggaggtgcc gggaatcgaa cccgggtcct tcgacactga
    59581 aacagggctt ctacgggcgt agcgcgctat tcgttttctc tgtcccggtt ctctcgcacg
    59641 ctagtaaccg acagactcag ttgtgaaaga tttccttacc gatagccaca acaacttcgg
    59701 ttagtaagcc cccctatcga cgttggattc tgaggcgggg agcgcgctca ggccaacgga
    59761 ttacttagct cgcttaggca gcgaggcggt aatcagacga agttttatct gcgtctgtat
    59821 gtgtgcacgg gtttttgtgg ttaacgagat catcccggct tcctcggccc gcttcccctg
    59881 gctcaacaaa cgaagtcgag accgttcacc cccgcgatta acgtggatta gaagctgtat
    59941 gcaaattata gagcagagat aggacttcaa aaaacctagc agatacgctc agtagcgcta
    60001 taactgagaa tcaatcttcg tatctctttg atttacccct actacttttt ccagttgagc
    60061 gatgagagag ttctcgatca atctcgcggt ttgcctgttt ttccatcagc gttgcacgct
    60121 tatcgtgggc tttcttgcct ctggctagtg caatctcaac ttttgctttg ccatctttaa
    60181 aataaagtga gagtggaatg agagttagtc cactttcctt taaggttcca attaggcgat
    60241 ttatctctcg cttactcatt aagagttttc tatttcgcct tggttcatgg ttggtccaag
    60301 agccctcgtt atattcagga atatgaacac cacttagata tatctcgcca tcttggacca
    60361 ttccataacc atcagtaagt gttgctctac ctgctcttag agatttaact tcggtgccga
    60421 caagaaccaa gcctgcttca aagacctcat cgatgaaata gtcatgtcgc cccttcttat
    60481 tctgggcaat taatttcttt cctatttctt ttaccacggc ttaagggtat ctctcctggc
    60541 caagatatcc ctgctaacta actgctttga gttaatcctt tgagataaag gatgccaaac
    60601 cgcctaagac ctggagtgat ttaaggccaa tttcagcagg ggctactgcc agatcaggag
    60661 ttattccaac ttcctcaatg atgcgacccg atggagttag aaagagtgca acagttagct
    60721 caagttttga accatcatca agtgaaatga gctcttgaac tgaacctttg ccgtaacttc
    60781 gttctccaat aactacgcct cgatttctat cctggaatgc cccagctaat atctcagcag
    60841 aacttgcagt gtttcggttg ataattacaa ctactggaac ctttattgga tttgaattta
    60901 ctgcacgaaa taccttttct gattcgttga ctttgtaaga aactatgatg ccactaccaa
    60961 taaattgctc agccacagca actgcctctt cgactaagcc cccagagtta tttctcaaat
    61021 ctattatcac gccagacttg taattagcac tctttagagc ctctgaaacc tcgttagctg
    61081 ttcctgaaat aaatgaggca atttcaacta gcgctaccga gctactaact tgtgtgagtt
    61141 caacactctt tgcctgcacc ttgccagtct ttatcgatgc gagaattctt tgctcattcc
    61201 taactacaag taaatcaact cgctttccaa tatctgctcg aataagagca atagtggaag
    61261 caagagatgc gcccgttact tgagtgccat ttacttcaac tagctcatct cctattgcaa
    61321 gatcagaatt tgctgcaggt gatccctctt gaatcgaagt aattttgatt actcccccgc
    61381 gggatttact caagtaaata ccaattccag taaatgaatt agagctctga gcttcaaagg
    61441 tttctagagc ctctcttgga aagtaattgg cccactcatc acctgatgct tttagaactc
    61501 cttcaatagc agcccgctct aatacatcgc gatctatgtc tttggcgcca ctttcaacta
    61561 cgctctttat cgcttcatct actgaactac tatttctaga ctcaccgacc tgaattccta
    61621 ttgcaaaaac tagcgaagtt attaatactg ctaggactgc ttttcgaagc tgatctgggt
    61681 tagttatcct ctgccgacta tttattgcct caggagaatc aattacttta gaaggattaa
    61741 cttcgctgac agttttcgac cttgtagcag ttgaatctgg ctttcttgct cttccaccaa
    61801 ttacagcgcg cttggaagca ggtgatttct tcttttcact cacttgaaaa tctcctattg
    61861 cttccttatt tgataaagcg ttctaattgc aaggcggtaa cggtagccca gaagcacatt
    61921 agaactcaac taagagctaa ttagcgctca gcgatagggc aagtagaagt tagactttta
    61981 agtactttcg aagagtcaat atagaagcta aggatgaaac taaaagtccg gttccaagaa
    62041 gataagcaga tacaacccaa acgtcattcc aggtaaagaa gttagtgaag gtcaaaagtg
    62101 gcgctattcg atcatcgatt acatacttta acgaagagag taggccaact gaaaaccccc
    62161 aaccaataat tgccgagaaa acaccttcga gaataaatgg aagttgaatg gagaaagaac
    62221 ttgcaccaac taatttcatt acattggtct ctcgtcttcg attaaaggca gcaattctta
    62281 gagtattact aatcaagagc gcagcggtta gcacgctggc aaatccaatt gctaacgctc
    62341 catcgcgcaa aacatttagt aacctaaaga atttctctag aattgacctt tgatcttgaa
    62401 ctacatctac tccaggccga cctgagaagg cgctctgcac tactgcaaat tgcgtagggt
    62461 ctgttagctt tacgcgaaat gactctggca attgatctgc agtgacattt tgcgaaatag
    62521 ctgaatcttt aaatctttcc tgaaagcgct caaatgcttc agactgtgat tcgtaataaa
    62581 ccgtttcaac aactggaagt tcttcaagat ctgcctgaat ctcaagtctc tgctctgagg
    62641 taataattcc accagcgcag gagggtgagt ccgagagaga gccacagaga tagaccgaga
    62701 cttcaatctt gtcataccag taatccttca tcactcgaac ttgagaattt gaaagtaggc
    62761 caataccaag aagtgatagc gagatagcaa ccgttactac gacagcaaag gtcatagtta
    62821 gatttcttcg taggccaatg cctacttcac taagaataaa tccagcgcgc atcgctaccc
    62881 cgtatatcca taaactccac gaacctgatc gcggataact tgacctttat ctaattcgat
    62941 aactcttttg cgcatctgat caacaattcc agagtcatga gtagccataa ccacagtagt
    63001 tccctcgcga ttgattcgat caagtagctt cataattcca acagaggttg cagggtctag
    63061 gtttccggtt ggttcatcag cgataagaat cgcaggattg ctcacataag cccttgctat
    63121 cgcaaccctt tgttgctccc cgcctgagag ctctccaggc tttcgatcag atttatcttc
    63181 aagaccaact agctctagaa cttctggaac ttcacgagca atttctttct ttgaaaaacc
    63241 tagtacatgc aaggtgaagg ccacattttc ggcaactgtt ttatttggta gcagacgaaa
    63301 atcttgaaat accgtgccaa cttggcgcct tagctcagga accttccaac tagaaagtgt
    63361 tcccaaatct tttccagcaa catgaatcgt tccagaagtt ggtcgctcct ctcttaagac
    63421 aagtcgaagg aatgtagatt ttccagagcc ggaaagtcct accaaaaaga caaactcgcc
    63481 cttctcgatt gcaatcgaga cgttatctaa agcgggtcgg tcgtttttgg cgtagccctt
    63541 agttacgttc tcgaaacgaa tcacgggtgt gattttaggt agagctagtg agaaagctgc
    63601 ggataaaccc gcggaattag ggtgagaaat aacgcagaat taccaattga gcgtataaat
    63661 cctgctttat tttctctctc tacgccatcg aattccagcg gagataaatc catcaagatc
    63721 accatctaga accgcttggg tattgccagt ttcaaaatca gtgcgatgat ccttgaccat
    63781 ttggtaggga tgaagcacat aggatcgaat ttgattcccc caagaacctg atgtatcacc
    63841 gcggagcgca tcaatttttg ccctctcctg tgcattacgt ctttccagca attttgattg
    63901 tagaaccgcc attgcagttg ctctattttg aatttgagat ctttcattct gacacgaaac
    63961 gacaattccg gtggggatgt gagtaattcg aactgcagaa tcagtggtgt taactccctg
    64021 ccctccaggc cctgatgatc gatatacatc gattcgaata tctttctcat caatttcaat
    64081 atcatcgctt tgatcaacaa ctgggacaac ttcaacgcca gcaaaggatg tgtggcgccg
    64141 attctgtgaa tcaaatggcg agattctcac caatcgatga gtcccctgct caacagagag
    64201 ggtgccataa caatatgggc cacttacctt aaaggtagtt gacttgattc cagcctcctc
    64261 accataagag gtttctagaa cactaactcc ataattgtgg cgctcgcaat atctgaaaaa
    64321 cattcttgag accatctcag cccaatcaga tgcatcaaca ccgccagctt ctgatcgaat
    64381 agttataagc gcatctcgat ggtcatattc accacttaat agtgtctgta tttcaagatc
    64441 tgatattgct gaagtcaaag aatctaactc ttttaggatt tccagttctg ctccatcgct
    64501 tgactcatta ttggcaagtt ctaacataat cggaagatca gtcactcttg atctaagact
    64561 gcttacctta tttatagtgc tatttaccct tgagagagcg ctcgttactt tctgcgctgc
    64621 ttctggatcg ctccaaagat caggaactcc tgcttgcgtc tctagctcca cctgctcttt
    64681 tcttaactta cctaaatcta aaacggcttc tattgatttc aaagtagcat caagggcagc
    64741 tatctcacca ttaatatcgc gctctgccat gggttaagga taaccccaat tattgctgtc
    64801 atagtcattg atagatgctc tccatcgata cctctatttg attggtaaat tcggtgatgc
    64861 caaagacccg caacttaaat ggcaattgat gaacctgttg caggctgaca tgaagactct
    64921 ttccatcaca gataaatgat tttaactcaa tgtcactatt tgcttggtta aagcttcgac
    64981 cgtagagatc caacttactc tttgctatca acgctacttc ttggctaaag gtcctgcctg
    65041 catcactgca attgatagga acggctctgc tctgaccgag aaatgtctct ggaatgggca
    65101 cttgatagta gtaagcaatt tcatctaact cctgaaccgc tctagatagg gcgccttcag
    65161 ttagattaat caaatctctt cttgcgatat aaaccgaggc tatatttgaa atgagaaagg
    65221 tcaaactcat cgcgataatg aagtagccca taatcaaggg agaaattgag ccatattcat
    65281 cctccaattg cgttctaact ctcatcacgc catttatcaa catagcctct tacaactgca
    65341 gcagcctttc gattctcacc tgccactgat ttatcaaagt catgaacttc aatttcaaat
    65401 gaacgataga gactcaattc aacaagtgag ccaggcttta gacaaccttg gctctggcag
    65461 gaaatgttgt aggtaaaacc agggcgcaga tcatcactgt tgcgtccttt attttctact
    65521 tcttgatatt gatcaagtag aaatcgaatt ctttgatgac ctagcgacaa gttatctgct
    65581 gttataaaag ctctaaggct ctgccttgca agcgttgaga ctgtcatctc ctcttttgcg
    65641 ctctgctgca tagatgaaaa gaaaattaga gcaggtatga atagcggcgt tgcaataagt
    65701 acaaattcag caattgctga tccgctctca tcgctatttc tattcacgaa tggctatgcc
    65761 ttcactcttc atcttgattg ctgaaccaga taaatttgtg atgactggaa tatcaagtgt
    65821 tgagctgtag gtaataatct cgcccccacc aggaagcgct aggacttctt gatcaatctc
    65881 ccgcctacca tccagagcat aaaaggctgt tttccctgcc tctgctgaaa atagtgcagt
    65941 gcgatttatg gcatttgaaa tatcaatttt atccagcaac tgccaacttc cagagagcat
    66001 taactggaga accaaaagga aaaaagcagt tgtaggaatc aagctaagag ctgactccac
    66061 cgaaccgctc tctaaatcgt gattatcact gcatttcatt aacgaatgcc attcattgca
    66121 tccaatgaat tttttaaaat agtactaagt cttggagcag caatagtcca aatagcagta
    66181 actaatcctg tggtcattaa gaccactaat acccaaccag gaacatctcc tgattcatca
    66241 acatcttttg ccataaattc tcgatacttc tcagcaactt tagatttatt caataaatcc
    66301 ttagactcta tgaattttac ttggaaaact tcactagata aaagcttttt gatttccata
    66361 tttaactccc tcttgtttta tcctttctgt aattctcacc tacctcagac atctgcttta
    66421 aacagagctt agatattgtg gagaaagggc gctgtgtata acttggttta atcacatgaa
    66481 actattgagc tgctcaaatg aaggaaatag tgcaaataaa attgagatag gaaggattaa
    66541 aaagacaatc ggaatcatga gagcaatctc agccttacct gctctctgca agatcaaatt
    66601 cttgctcgct agacgagcat ccttaacctg agaaatcagg ataggcccca aagatgagcc
    66661 tctctcaata cctagaacta tgctggtagt aaatcttctc aatactagag agtcatagcg
    66721 cagagcgaga ttatcaaggg cctgggtaag agatgtgcca tttgagattt ctgaaaccac
    66781 taggtctagc tctcttgcac ttaatgatgt tgatctctga ccaattattg caagtgccct
    66841 agagggcgag attccagagg agataagaat agttaaaagt tgaattagcg agggaagttc
    66901 tctttcaaag atctctgtcg caactcttct ctctgtatct gctcgctttc gaagtagtgc
    66961 aagaaataag gccagaatgc tcagtagtaa gagtgaggct ggaatctgct caggccagat
    67021 ggcaatcaat aagtagagtg caattgtcgc tgcgctaagg attaaaaagt aaatcccaat
    67081 atctctaact ctacttgcct tatgaccaag actttccact ctaattaccc ttgaatactt
    67141 ttcacttctt gaacctaaat tcgcaaagct ctcattgcta attatccaaa ccccaagcaa
    67201 catgagaggc aaaacggaga gaagcgagag cagacgtata tcagttaatt ccgctttcat
    67261 ctcaacgccc tcggcgcagc tggaagagag ccgaccttct ccatccaaat gtaggcagtt
    67321 gcagtcatca caaccccgag agatagaact aaggctccac ttggcgtgga gtagatttca
    67381 actgtcgaat cttgagtgga gagaaggatc aataagagcc agggcgctgc agttgctaaa
    67441 agagctgaat ttctaatcca accaaattta gttcgaatct cctcagctac tgcaatatct
    67501 tctctaacaa attcggctaa caaacgaagg gcattattag catcccttcc tccaaagtcc
    67561 tttgagataa ttagagtttc aaagacctga tcagagattg ctgaatctag gcgtctcttt
    67621 tcttttagta aaacctgttc aaaatttcct aagcttaaaa gattgctctg aatctgagca
    67681 aagcttggtc ttaatacctt tggaccatgc agcgctaatt gagtgagcgc ttcagagatt
    67741 gaaatgccgg cttgaagcgc tgagactaaa gagtcaatcg cctccggcca ggcaacttct
    67801 tgctctctct gccttttgag atttcgccct cggccaataa gaaaaggaag tgctgctgag
    67861 agtgtggcaa ttgctataga aattgcctca gatgaggtga gcagaaaggt agtgaaatat
    67921 gaagagatta gagtaattat caaagttata acgcttcgag aggagatctt cttatctggc
    67981 ttaagtcttt tgtttagaga gcttaggtca aataaggcga tatatgagcc aagggctgca
    68041 agcaaggccg taaagaccgg cagagcaaat tgctcaaaca tggaaacctc tttgatattg
    68101 cccctggctc caggtaaata tcggctctaa atcgatatga aagttctctg ccctatctag
    68161 cactttaacc acttgcagaa ctcttcgaac tccagtgcta tcaagaccaa cgtgaatcac
    68221 ataatcaagt gctcgaaata ctgttggggt taagaaatct tgggtgatgt tttcgccagc
    68281 tagaagcggg agcgtctgaa gttttcttat cgcctctgtt gctgaattag catggattgt
    68341 tgccattcca ggaattcctg agttgagcgc aatcaacata tcccaagatt cagcctcccg
    68401 aatctccccg attaccaaac gcgatggacg cattcttaac gcttctttga ttagctttct
    68461 aatgcttatt tccccaactc catcaagact tgcgcttcgg gtctgcatcg ctacacaatc
    68521 tggaattctt ggacttagct caaagacttc ttcaatagtt attactcgat ctgttattgg
    68581 cactgcgctg acaagagcgt taagtagagt tgttttacct gcttgagttg aaccacttac
    68641 caatatgttt tttctatctc ttaccgcctc gccaagaaac tctcttatct cggaagagat
    68701 aacttccatc tcaactaaat taagtaagct cttgccacgc attaagtgct tacggatatt
    68761 tatcgcccaa tgctcagaag taacttcagg aataacaatg tggagccggc ttccatcagg
    68821 taggcgggca tcaacaaagg gatggcttag atcaattctt ctgcccgacc acatcaatat
    68881 gcgatccata attgattgaa gctctgaagc gcttaaaact atattagtta actcactctt
    68941 acctgaacgg gcgataaaga ttctctgcgg tgagttgatc cagatctctt cgacttcatc
    69001 atcttctaga aggctttgta gcgctccata atcagcacct tggtgggttt cttcattttc
    69061 catggccctt tcataggcca agatcaggca aactcttgct tatttattca ccacagttgt
    69121 gaattaaacg cagagaagaa atgcgtagtg agcacctagg cactctcggt atactcggtc
    69181 ccgcaatact ttctattgca tggtgggcgt agctcagctg gtagagcacc cggttgtggt
    69241 ccgggatgtc gcgggttcaa gccccgtcgc tcaccccatt aacactttag ttacagcgct
    69301 gtttaagcag ttgctaccaa aaccttttca tcaattaatt cagaagtgac aacttct
//
LOCUS       DAOROD010000005        60936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_14_length_60936_cov_31.976084, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000005 DAOROD010000000
VERSION     DAOROD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 60936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60936
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_60936_cov_31.976084"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLW53_02125"
     CDS             <1..452
                     /locus_tag="PLW53_02125"
                     /inference="COORDINATES: protein motif:HMM:NF022674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HRD36255.1"
                     /translation="DVVLLPGAGALLAPDWIPYSSRITAGDVGVGTIVPTALDDPRLV
                     PAASALPGDEELDLHELFEFGAGRNRILSIEGRDQAAKRWISGDRGPDTPMAQFAPKN
                     CGTCGFYLPIAGSFRAAFGVCANAISPEDARVVAVNHGCGAHSEAIN"
     gene            520..900
                     /locus_tag="PLW53_02130"
     CDS             520..900
                     /locus_tag="PLW53_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRD36256.1"
                     /translation="MPVGRVKWFSLEKGFGFISSDEGEDVYLAASALPEGVSTVKPGT
                     KLEFGVAEGRKGPQALSVRVIEAPPSLANSKAGADDLAVIIEESIKMLDRVGNGLRHG
                     RYPSDAEAARVAKVLRGIAQEIEG"
     gene            complement(897..1082)
                     /locus_tag="PLW53_02135"
     CDS             complement(897..1082)
                     /locus_tag="PLW53_02135"
                     /inference="COORDINATES: protein motif:HMM:NF022205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HRD36257.1"
                     /translation="MIADMQSAIRVIIFGIVLWSIALVVAIVVNAVAKIILTCLLAIL
                     LGFIGIYYTKAREKKAR"
     gene            1097..2350
                     /locus_tag="PLW53_02140"
     CDS             1097..2350
                     /locus_tag="PLW53_02140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36258.1"
                     /translation="MGTLRSFKHRNFKILFSANFLSNIGTWAQGVAQAWLILELTDSG
                     TYLGIVTSLQFAPILFFSISGGKIADKFNKRKVLMLTNLTAGISALSVATLVLTENIK
                     IWHVMFFAFMLGMGNAIDAPVRQSFNVEVVGKQDLPNAVGLNSTNFNIGRLIGPGLSG
                     LLIAAYGTGVSFLLNGISYLVVIIALINIRESELFIEEKKSSSTKIREAMAYVAARPD
                     ILAVMITVFFATSFGLNFNIFNTMMATQVFDKGAAEYGALGSILAVGSLSGAIISARL
                     EKKRGPRFVMIGSMIFSAILILSAFAPTYLVYSILLPIIGCVALLTFIGANTMVQLRT
                     DSQIRGRVMGIYLTVFLGGTPIVSPFIGIMTQEVGTRPTVAICGLISLLAAIATFAKF
                     KDKGQEPDSFAIADVLEPTYENKKD"
     gene            complement(2408..3529)
                     /gene="serC"
                     /locus_tag="PLW53_02145"
     CDS             complement(2408..3529)
                     /gene="serC"
                     /locus_tag="PLW53_02145"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR01366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRD36259.1"
                     /translation="MSEIKIPENLKPSDPRFGCGPSKIRPAALQVLAGPGAKILGTSH
                     RQKEVKSVVSRVRSGLSSLFDLPQGYEVVLGNGGSTAFWDIATFGLIEKKSQHLSFGE
                     FSSKFAQAAKEAPFLDEPSVIKSEPGTHPQAVVEAGIDAYCLTHNETSTGVAMQIKRP
                     AKSDGALVLVDATSAAGGLSVSPSEFDAYYFAPQKSFASDGGLWISLMSPAAIERVAK
                     IKSSGRWVPAFFDLTIAIDNSRLDQTYNTPAVATLILLAEQIEWMNQGGGMAFAAGRS
                     AKSAEIIYSWAEKTSYTTPFVTDPAMRSNVVATINFSDDIDALEIAKVLRANGILDTE
                     PYRKLGKNQLRVGMFPAIDPEDIKALTKCIEYVVENLKK"
     gene            complement(3542..4225)
                     /gene="pdxH"
                     /locus_tag="PLW53_02150"
     CDS             complement(3542..4225)
                     /gene="pdxH"
                     /locus_tag="PLW53_02150"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRD36260.1"
                     /translation="MTLDTPMAESPEISREAISAMRRSYGEVGITESSINPDPIAQFS
                     LWLKEAAANSMIIEANAMVLSTLGQDGPSSRTVLLKDVDQNGFTFFTNYQSDKSRQIQ
                     ANPKVSLLFPWYPMERQVIVIGSASKIDSSESEKYFATRPWSSQIGALASSQSEVIES
                     RQVLEERFRELAEKYPQGSVVPKPENWGGYLVKPNSIEFWQGRYSRLHDRLRFNLSGS
                     NWSLERLSP"
     gene            4295..5410
                     /locus_tag="PLW53_02155"
     CDS             4295..5410
                     /locus_tag="PLW53_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HRD36261.1"
                     /translation="MNEDFKPGLEGVIAFESEIAEPDKEGSALRYRGVDIDDLVGRVT
                     FGNVWGLLVDDEFNPGLPAAEPFPIPIHSGDVRVDVQSAIAMLAPAWGLKPLLDISDE
                     EARSNLARASVMVLSYVAQSARGTWQPMVSQAEVDKAHTVVERMMIRWRGEPDPKHVR
                     AIDAYFVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPSRVLHMI
                     EEVERRGDARAYVKELLDKGERLMGFGHRVYRAQDPRSRTLRRTCEELGAPRYQVAKA
                     LEDAALAELRERRPDRVLETNVEFWAAVLLDFAEVPGHMFTPMFTSARTAGWSAHILE
                     QKRTGRLIRPSARYIGKAPRRPEDVKGWDESVSGLHL"
     gene            5573..6826
                     /locus_tag="PLW53_02160"
     CDS             5573..6826
                     /locus_tag="PLW53_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD36262.1"
                     /translation="MKKKFFRGFAVLATLALAAGLAVTPAHANKNIVVWADESRGPNL
                     TKTLATKGDWVPGYTVTVKSFANFDALKDAIDKATATTGPDIVVGANDWVPTLAKNGK
                     LAPLTLTASQRARFSASQFYDLSYKKKLYGIPVDVNNVAMIYNTKLVTSAPKSFGEMV
                     DYYKANKTSKSLKAGLCVAGGGMSWGAHSVFSALGADAYYFNPNGTINKSKSFNPNEF
                     GANVKKFLLDGKKSNGFFPATDTGCKDNFLAGSVPFAIIGNWEWNDYKAKGFTMNLMP
                     VPGASSGKSGNAFGSVSGALLTTFAASNGVEAAAKSLLVDFFGSTAGQVAYQLNEKRP
                     PAEKGAASDATVTDGQKGFGASAAAASIPQVGAILNGPSGKSYWDSAPAYWTAVLVDG
                     KDAVTEAKKLAAIWRANLKAAYNDL"
     gene            6959..8485
                     /locus_tag="PLW53_02165"
     CDS             6959..8485
                     /locus_tag="PLW53_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD36263.1"
                     /translation="MLIRGKVATFIKVAIVALISALLLTLTQSAFAKKEYVIAAFLAV
                     AIFAINFTYFNKRTVPLKFFLPGILLLSAFVVTPILYTVVMSTYNYKTGNYISKEEAI
                     GRIKALALEPDENNSAFDIVVGKFQESEAILVSDIENKSYFISTPERRLELQIDQVEL
                     DENFVAQRANGFTPLEAGEIASSKAATIRYFYNSQYFITVENLNVGAVFRQSLEYLPN
                     QDIFKSLINDSIYRDNGNGNYVNPEDKSDFLEPGWRDPAWFTNYIRLVSDERVRDPLI
                     SVFIWTVVFATLTVLTQFSFGLLLALALNKPIRGRRIYRSILVLPYAMPSVMSILIWG
                     GMFNTEFGAVNEILGSDIAWFQDPTFARFAVILVNLWLGFPYFYLVSSGSLQAIPSEL
                     LEAAAIDGANPRQVFSKVTLPLLLQILSPLLIASFAFNFNNFNLIYLLTGGGPREQLD
                     GEIAGSTDILISYTYQIAFGSNLQDLGLASAISVLIFFLVASISLYGVRKSKVLESFT
                     "
     gene            8482..9333
                     /locus_tag="PLW53_02170"
     CDS             8482..9333
                     /locus_tag="PLW53_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD36264.1"
                     /translation="MTKWFRENLWRHITGTVMCLFALFPLYLVLLSAFNESGSLQVTS
                     FLPREISFANFDVLFNDERIPYLTWMKNSLIISGFVAVASVVIGAASAFAFSRLKFQG
                     KKFGIQLLLLIQMFPAILALSALYVIMERVYKFAPGLGIGTQLGLVLVYLGGAMGVNI
                     WLLKGFVDSIPAELDEAAKIDGASAMQTYWQIFVPLAAPVLAVVSLLSFIGTFNEFIL
                     ARLFLIEVESRTVAVGLQQFIGGQYSENWGPFAAGSIIASIPIVIIFLSLQKYIISGL
                     TAGSVKG"
     gene            9333..11282
                     /locus_tag="PLW53_02175"
     CDS             9333..11282
                     /locus_tag="PLW53_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRD36265.1"
                     /translation="MKLSRLALFALLALLLVPFTSLHAQTPKVDIARTGIDKEVIYFV
                     MPDRYRNGDVSNDDLPGFNPTHTAFFHGGDLKGLTGNCVDDDGLARLKKLGFTAIWLT
                     PLVVQQPPTDGGAGYHGYWGVDFLGVDPHLGSKEDLMALRKCAEKLGLKLILDVVTNH
                     TGDVVWYNNRDAYIPDKYKNIKNPSWLNEISNYHNYGDMNSCWSPGACVRDGDFFGLD
                     DLATEKPEVYNGLAGIYSDWIEKYGFVGFRVDTARHLDDQFFKNWSPQINQIAEKSGI
                     ANFTIFGEVWEPSPIALMPYIRVNKMQSALDFPFQRVAVDYAASSSNASILKNLFAYD
                     DYYTSATTSASNLVTFLGNHDMGRANFLIERVKVNPPGQLLSRVKLANTLLYLSRGVP
                     TVYYGDEVGILGSGNGRDQLARQDLFPTKVEIWKQEPRVTGKPVGSADSFVETFSNPI
                     AKHLITLSELRKAHPALANEQMQIRYAKGSVFAFSKRAISEKREYLVVLNNSAKTSTV
                     KISTATSGKWKDLISGKSFTSKSGELTLKVDGLSSAVLRAESEINQRGIKLGKLSAKE
                     DFLSGFYNISLKVESKDLAIAEFFIRTKADSKWSTLGVDLNQPFNVFIDPNEYSGPIE
                     VKANVIDSKGGKNELREIAFNIATP"
     gene            11242..13047
                     /locus_tag="PLW53_02180"
     CDS             11242..13047
                     /locus_tag="PLW53_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRD36266.1"
                     /translation="MSFEKSLSILPHHDGSELYLSSSSPTLQERVTFKFRAGSNFPIE
                     QAILRLYHDGEPRFFPMKRSIIKGEQWWQVKVEIRNLKTPYRFLIVNGDTYSWLNARG
                     FQSHDVTSANDFQLLATPAFPKWIRSAVFYQIFPDRFATTGKYQHLKPEKFISRPWDQ
                     LPKGRDKSTGVEFFGGDLDGVSQHLKYLKKLGINGIYFTPIFPAGSTHRYDASSFDEV
                     DPLLGGDAALIRLAKKAKSAGIYLMGDLTTNHCGREHRWLQRSLKSKKAKEREFFYWD
                     KSVKHGYEGWWGLASLPKLNYNSKKLRELMYEGKNSIVKKWLKKPYNLSGWRIDVGNM
                     TGRYKGEDINGEVIAGVRKALQESNSQAWLVAENADHFPSDLDGFGWHGTMNYNGFMR
                     PIWGWLNHRAEVEKAFFGVPVSIPRFTAGEMVSAMKEFSSTIPWRNFVSSMLLLDSHD
                     TARFRNVVGKDTKRHIAGMGLLLTYPGVPSIYAGDELGVEGQWGEDGRRTIDWSRQSW
                     DHEFLSEVSKLIKIRRQSHALAQGGLRWILIEDDLLAFERESKREKLLVVVSRSAQKC
                     KIEGIGEVKQRLYGPELKGQIYKSDSASLGIYRLF"
     gene            13094..14449
                     /locus_tag="PLW53_02185"
     CDS             13094..14449
                     /locus_tag="PLW53_02185"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRD36267.1"
                     /translation="MSKRSIFWFRRDLRLADNPALLACLDEADEVVPLFILDDEISER
                     AGDHRRAFLANSLRALNDSLGGALHVISGNPVEVLSNLMKRYETTSIHSAFPFAPYGN
                     ARDEELRSAGIKLEQLGSSYAVAPGRVLKGDGTPYRVYTPFYRAWLAHGWRAPVAAPK
                     SPNWVTPKKSDQNIPNWKAPEGIVLQEAGEAAALTRFKKFLKDSAADYGDNRNIPGIE
                     GTSKLSPHLRWGEIHPRTILAALGQSKGHEVYRKEIAWREFYADVLHNNPHTSRDYYD
                     PKYKKMRYEKPGKAFEAWASGKTGYPFVDAAMRQLVKEGWMHNRTRMVVASFLVKDLH
                     IEWQHGANFFMKYLIDNDVASNSHGWQWTAGCGTDASPYYRVFNPIEQGRRFDPEGIY
                     IKKYIPELSHLQGDEVHEPWESKDGYKNGYPKRIVDHAAERLESLARLEEIKAAKPLK
                     P"
     gene            complement(14453..15886)
                     /locus_tag="PLW53_02190"
     CDS             complement(14453..15886)
                     /locus_tag="PLW53_02190"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRD36268.1"
                     /translation="MAEKFKNKVLILGAGSVSQSVLPLLIEHLVDAKQITIMDQRDNR
                     SRVKGALDKGATYIQDQITKDNLDSQLKKYLSTGDFLLDLAWNIDANTILNWCHDNGV
                     LYLNTSVEEWDPYAGGANKHPLERTLYYRHMRMREMKSKWNKVGATAIVEHGANPGLV
                     SHLVKKSMVDIATLALKESKAAAGVESALTDERYNDLAHLLGVKVIHISERDTQVTNK
                     PKQWGEFVNTWSVEGFYEEGIAPAELGWGTHEKTLPLNAYEHQSGPKNQIAIAQPGAT
                     TWVRSWVPNFEIHGMVIRHGEAFTISDHLTVWDGAKALYRPTVHYAYCPTNEAIASMK
                     ELEMRSWDLHKNQRIMNDEITDGDDRLGVLLMGHPYKAWWTGSLLSIHESRKLIPDQS
                     ATTVQVASAVYAAVAWAMKNPDKGLLVPDELPWREVLSYAEKYWGGIHSEASAWDPLM
                     NRKDLFEGWNGKKVDSSDPWQFSNFLV"
     gene            complement(15981..17393)
                     /locus_tag="PLW53_02195"
     CDS             complement(15981..17393)
                     /locus_tag="PLW53_02195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRD36269.1"
                     /translation="MKIYLLFRLLAIATLIAYLFLRNNSDARWEYDLVLFNLVALLAA
                     LSIALSPILDDPLGRAGVIGAIISWSAGSIASSLDSFFEINTLLDLDLIAQSFYALFY
                     PLAIFGLTRAIRFKTLSKSLELLDTSIIAIGYTTILTALLVRPAMTTIDGSIFDVFLA
                     ILYPVGDIVILVLVLLLVLRQSMSWRNGLILIGATIFFLSDFYFLYQSYLGKYEFGSL
                     TDVGWLISFILLSQAFWFANNEELAARSFNSAVATIALLGSSTLLAIAVLQPSYIPRF
                     LILPAFVTIALSFLRMGVAINDARNMSNEQILARTDELTGLANRRKFMVDGQEFLKEP
                     GSLLILDLDGFKTVNDSLGHGIGDQLLKQVANRFQRVMPSDGLLARLGGDEFGALIPG
                     GDGIEVARALKATLTYPFHINSNEIHLDVSIGEARNDPGSSALEQLLRRADEAMYEAK
                     RSKLGVVSWHNQLGTSGSRL"
     gene            17420..17845
                     /locus_tag="PLW53_02200"
     CDS             17420..17845
                     /locus_tag="PLW53_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRD36270.1"
                     /translation="MDSEKNVSRRAVLCGLAVLTLGLIPENAIAASGVKVLANGKVEV
                     TLSKNPALKKVGGVVQFTNNQGIDIALVRTGKGSSAFKALNLACTHQGYPLRKDGNKW
                     VCDNHLANFSITGAVTKGPAESPLQNVALKVTTTKVIVG"
     gene            complement(17854..18552)
                     /locus_tag="PLW53_02205"
     CDS             complement(17854..18552)
                     /locus_tag="PLW53_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36271.1"
                     /translation="MRNSLKQFGRGATLFAATSLLMATTAVIPAEAANKAGAACTKAN
                     AKTKIGGDGYVCTKNPIVKNAKLTWVWVGCIDSNKLYLESSARLKSITETAAQAATML
                     DTEIAALKAAAPADEAEAKVFDQKATDAKAKQAAALLEAKANTDNATKVGATTTAGKQ
                     YTTNAATWTKAARSYELAAKNFERSAASLRDKIGEVAKKEKQKVNVLQTVENTKSEVS
                     STLQNRKQACKPGL"
     gene            complement(18670..19452)
                     /locus_tag="PLW53_02210"
     CDS             complement(18670..19452)
                     /locus_tag="PLW53_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD36272.1"
                     /translation="MSKPIALITGATAGIGASFAELLAKKGHDLVIVARDLERLNQNA
                     SQWRENHGVEVEVLVADLSRDEDIRRVELRLQNTSRPIEVLINNAGFGIKKSFLVSEI
                     SDEIALLDVLVTAPMRLMHAVLPTMKARNSGVIINVSSIAGWIASGTYSAAKSYLTVL
                     SESLHTELSSSNVKVLALAPGYTRTEFHQRGNMKMNGLPNWLWLTSEKVAQDAWDSAQ
                     AGKAICIPGRQYQVISKLARYAPRSWVRKLGMNLGSRRGRNK"
     gene            complement(19452..20678)
                     /locus_tag="PLW53_02215"
     CDS             complement(19452..20678)
                     /locus_tag="PLW53_02215"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRD36273.1"
                     /translation="MKALVIGAGGVGRAIANIAVRRPFITSMVIADRTLSRAEEAVYR
                     VKDPRFSAAQVNAAELEDLRELIRKVDPDVVINAVDPRFVMPIFLACEIENTNYIDMA
                     MSLSRPHPHYPHRETGVKLGDEQFARDWNWEERKIYALVGMGVEPGMSDIFAKYANDE
                     LFSRIDSITVLDGSNLRVEGADFAPSFSIWTTIEECLNPPLVWEDGRGWFTTEPFSEI
                     EVFDFPEGIGPVECVNVEHEEVVLIPQKIDAKKVNFKYGLGAEFIQILKTINMLGMDK
                     KENVDVQGVSVSPRDLLAASLPDPATLGSRMKGKTCAGTLVKGLDKSGNPKAVYIYNV
                     IDNQWSMKEYGDQAVVWQTAVNPIIAMELIEKGIWKPRGVNGPEWFDAKPFLDLLEEY
                     GTSWHIREEDASNIVK"
     gene            20837..20909
                     /locus_tag="PLW53_02220"
     tRNA            20837..20909
                     /locus_tag="PLW53_02220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20870..20872,aa:Arg,seq:cct)
     gene            complement(21145..21555)
                     /locus_tag="PLW53_02225"
     CDS             complement(21145..21555)
                     /locus_tag="PLW53_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36274.1"
                     /translation="MRKGLLARSAVAALAFSLMSATGAIAQDKSAGRNPVDKAEKVKA
                     ITDAIGIDSESLKTKRQAGESLATIAGSKVDALLAALLAFDSKKIDAAVASGKITTIQ
                     ATELKAKLITRVNEVANRIGKKVETDKSQRSGGN"
     gene            complement(21841..22764)
                     /locus_tag="PLW53_02230"
     CDS             complement(21841..22764)
                     /locus_tag="PLW53_02230"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD36275.1"
                     /translation="MTTEIIPVFSGGVGRSGTTIVGRILKQHSNVFAGSPFEVKFITE
                     THGLIDLVFGLRKFLPTQISRKGYLLSKLSQHDPIQLRFVKFRKRIYEDWWIRTNRLG
                     KPSGLHRALGISQMEELLDELGSTLNTPMEAARKFVFGYVRNHYKWNGENYWMDTTPA
                     NIMYADFIHRIFPEAKFIEMRRNALDNISSVLKEPWGPNNPDRAIIWWRDRVFLATEA
                     KKLVAENQHLTLFLENLVRENRENCYRRLISHVGLSDELEMRRYFETEVTAERAHFGR
                     WRRDFKNPEEFQARFESLISQELSQQQYLLP"
     gene            23001..24560
                     /locus_tag="PLW53_02235"
     CDS             23001..24560
                     /locus_tag="PLW53_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD36276.1"
                     /translation="MTYDSKTLLHYWSQKPLSRRTLLVTVVVSTFANSAIGKAIGATA
                     PIANFILGRPTSNSIALNILATEKVRAYVEYGYTKSKYTEKTSIDALETGVPKVIELK
                     NLKASSKVYYRIRYAAGSAKTFQTSKQFSFSTAKKVASPFTFTVHGDTHPERAGKMFN
                     SDLYYVTMANVAEQQPDFHILMGDDFSIDPLISKGQANQANVEKIYRTHRDWLGVIGS
                     SVPIFLVNGNHEQAAAYLLDGTTTNPAVLAGNARLKYYPLPAPNDFYSGNSKEIPGVG
                     LPRDYYAWTWGDALFVTLDPYWHSKNAVDNVAGVLVADDASGATKGGKKNSASGTQGG
                     NTGGAGGGGGNSKNLWGIGIGDEQYAWFKKTLEESNAKFKFVFAHHVMGTGRGAVELS
                     YDYEWGGRDPKGVTSFEKERPNWELPIHDLMVKNKVSIFFQGHDHIFVTQERDGIIYQ
                     SMPNPADDTFSYFNDAAYKSGTKAPNSGHVRVSVTPNLATVEYFLAARSKDSGRKNLQ
                     VAHKYTIPSKI"
     gene            complement(24569..25789)
                     /locus_tag="PLW53_02240"
     CDS             complement(24569..25789)
                     /locus_tag="PLW53_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD36277.1"
                     /translation="MNLQIRLILLLSSIVTLVSASIGTFAVSQSYQQQLSLNDIKIEN
                     VILQLKQSSEDPLTVATFIADQSDFPFSLSLITEDLQVVVVIDKSGEISDSVDLNFLE
                     RASDKPINLGESRVRSLKLSGAEYLVVGYSLSELRDLRGKNQLYLGIFTILVLIVAIL
                     FSYFFFRRDAQLNRAARALDENQERMLEFLGDAAHELRTPLTVIRGYFDLIKGGRGDN
                     DKRQDYERHVDREIGRMQKLINDLLDAAELESKEMSLDSHSNVSTALNNQVAQLKELQ
                     PQRKIDTSIENNVVAVIEQEDLDRLLGNIFSNIKRYTPDNSPLEINCRHSGKLVTFEI
                     ADSGPGLPKKFYEDGVRAFRRFDSSRSRDAGGSGLGMSIIQKVVKRWGGRLELSPSKA
                     GGLKIRVILKLSQG"
     gene            complement(25789..26475)
                     /locus_tag="PLW53_02245"
     CDS             complement(25789..26475)
                     /locus_tag="PLW53_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD36278.1"
                     /translation="MASNTRILVVEDEQSIRDLIRDSLSLIEVDTDEAGDGLEALNKL
                     RRQSFDLMILDVNLPKIDGFALLSKIREEGSGIPVLVLSARSQRDEITQGLKIGADDY
                     LIKPFGIEELILRVKAILRRTKPASAESKKLICGPITMDQERHLVTFNSEKVELSATE
                     FSLLEGLLNRSGKVVKKELLLAEVWGIDFPSNTTVVETYISYLRRKLHRDGYQGIKTV
                     RGVGFQILGE"
     gene            26627..27082
                     /locus_tag="PLW53_02250"
     CDS             26627..27082
                     /locus_tag="PLW53_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36279.1"
                     /translation="MRRITLIALISLGVIFGSSTATFAEGNGGNKTAITAWKAENQAK
                     MDAYQAALKKYLEIKKANEEARKAIATKFKSDADALRASTKAAVEAAATAEAKKVIAR
                     ASKDSLEKLITERKVALEALPNPGAKPVKPTLTPKPAKPSPKASPSSRG"
     gene            complement(27231..28070)
                     /gene="purU"
                     /locus_tag="PLW53_02255"
     CDS             complement(27231..28070)
                     /gene="purU"
                     /locus_tag="PLW53_02255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRD36280.1"
                     /translation="MDLIATLQCADQRGIVHAMTSSVLACQGNIIENQQFTDPVTNSF
                     VMRTRFETSSSLDTAREVLFEGLSKFNPELTIRDNASPKRALVMVTKESHCLRDLLYL
                     LELGELPIQIPLVVGNHEELKSLVESHGIKFKYLPISIENKESSETQLLSLIDSEKID
                     FLVLARYMQILSPDFCDKLSNRIINIHHSFLPGFKGAKPYHQAHAKGVKIIGATAHFV
                     TKDLDEGPIIEQDVAHVSHASTPEELIAIGRDIERRVLARAVKLYAEDRIFIVGNRTV
                     IFN"
     gene            complement(28098..30200)
                     /locus_tag="PLW53_02260"
     CDS             complement(28098..30200)
                     /locus_tag="PLW53_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRD36281.1"
                     /translation="MFEKLGHLIAKRKKSVLTLFLLSTILAGAIGSQVFSRFDTGGYM
                     DPNSDSAKVWEYLDETFKVKDPAVVLVVDGKDKSADDPSVVAAAQKIEASLRSEPSAE
                     NVLSYWSAGGAPSLKSKDGNSAFIFVYLKSTDFTEIDKLGGFYQEKYDGAFEGVEVYA
                     SGGAVFANAINGRIQNDLKISEAISIPLTFLLLLFVFGAMAASAMPLIVGITAILGTF
                     LVMFLLTLVTDVSIFALNLTTGLGLGLGIDYALLVVNRFREELAKGLDNESAIVNTLR
                     TAGKTVFYSGVTVVLTLLSLTFFPQNFLKSMGYAGAAVVALAVVGALIPLPAILALLG
                     TKINKGVVRRGGIVQKEDGRWSKVARFVMRRPISVITASLAVLFLMISPIANIKFSQV
                     DSGVLPRDDRAYIASQFIADEFPGQESNPIEIVFPNGASQTSEINAFVNRVSLTEGIT
                     RVGQVELAGESARVVAIHSMQPRTSEGEALIKELRNLPAPSGTLIGGAAADYADTQNA
                     ISSTLPAVLGWIIITVLLLLFAFTGSILLPIKAVILNFVSLAATMGVLTWVFIEGNLK
                     FLVGDFIETGSLDTNTMVLIAVVAFGLSMDYEVFLLSRIKEEHDAGNSNIDSVAMGLQ
                     KSARIITAAAFILAVVFAAFVISGVTSIKMMGFGVAFAILLDATLIRAFLVPALMRLF
                     GDWNWWAPRSLKRFQINH"
     gene            complement(30335..30580)
                     /locus_tag="PLW53_02265"
     CDS             complement(30335..30580)
                     /locus_tag="PLW53_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRD36282.1"
                     /translation="MPTYQYQCTACEHAFEIVQSFTDEPISICPECGKEVRKIYSNVG
                     IVFKGSGFYKTDSQVKKSEAPKPTPTTSATPPPAKND"
     gene            30635..32419
                     /locus_tag="PLW53_02270"
     CDS             30635..32419
                     /locus_tag="PLW53_02270"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRD36283.1"
                     /translation="MFFDLANSALAVLVIGLLISLAFLLPENQGRLEQSASTTLQGLR
                     IVSGLWFLISIGYLLTSLAEIFGSGIGEILKVNILQSFITQITLGKLLAYQVIVALIV
                     FIFSNLIKKNGGALALLILALSGIVAPLFQSHSSSQGSHSLAIGSLVIHVIALSFWLG
                     SVIALKVMPSELQDFAFSRVSVIALWSSLSVVLTGIANAWTRLRLSQDWFTGYGALIS
                     LKVVLTLLVFFIASRVRKNLSVNTLVAFEIGIMAAILGIGSILNRFTPIERGEIEFDR
                     IRELVGISMPSEPTLSRVFFEYEANGLALGALIFVTALYIRGVVALARRGDRWPVGRT
                     ISFAIGISLLDYATSGGLGLYSHFSFQYHMIAHMVLSMIAPIAIILSAPITLALRTLP
                     IGRDKSERGIRGMLIQALHSRPSRVITHPVSALAIFDGSLFALYFTPLFSNLMSGHFG
                     HLIMNFHFVAAGLLFFHVIVGIDPNPRKVHHLVRVVILLAAMSIHAFFSVALMSANEL
                     IDGGFYQLLDRPWATDLLSDQKMGAAIGWAMGEIPIVIALVATFIQWVRSDAREAKRA
                     DRRSNTELAEYNAYLEQLSRKNNSSQDK"
     gene            32429..33577
                     /locus_tag="PLW53_02275"
     CDS             32429..33577
                     /locus_tag="PLW53_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD36284.1"
                     /translation="MENLFDLPEEYQALRASVRSLADKKIQPFAHDVDANSRYPQEAF
                     EALQGAGLAAAHVPTEYGGDGADALGVVIIIEEVARVCASASLIPAVNKLGSVPLMLG
                     GSPEQQKRWLTPLAQGKGFSYCLSESEAGSDAASMKTRAVRKGDGWVLNGSKKWISNA
                     GVSEFYTVLASTDPEKGAKGITAFIVEKSDPGLSFGAPEKKMGFKGSPTREVYLDNVE
                     ISDDRRISEIGAGFSLAMKTLDHTRITIAAQALGIAQGAFEVATKYAHERKQFGKPIF
                     DFQAVQFMLADMAMEIAAARQLTYAAASKSENSASDLTFFSAASKCYATDVAMKVTTD
                     AVQVLGGYGYVSDYPVERMMRDAKLTQIYEGTNQVQRLVMARNLNSLL"
     gene            complement(33561..34124)
                     /locus_tag="PLW53_02280"
     CDS             complement(33561..34124)
                     /locus_tag="PLW53_02280"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRD36285.1"
                     /translation="MDSSQSASLIKSELRKRFRSEREFHLSECSWQHLLDSKEIKSAE
                     VLASYYSYGSEPDTSELNNSLIQSGKTLLLPRRLADNDLDWIIWSGKDEDLEIDGNLK
                     SPKGESFKGKIDVVIVPALHVDRQGNRLGQGGGSYDRALAKLNAWKVALVYSGELTSE
                     SLPVESFDCKVDAAATPEILVRFTAKN"
     gene            34158..35081
                     /locus_tag="PLW53_02285"
     CDS             34158..35081
                     /locus_tag="PLW53_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36286.1"
                     /translation="MSETIKVAELATELLALAKPLAAFDMPLLDAHGATLALDVSSGE
                     QVALKSGSRIRATQIGLAASLGLDRLPTRPQPRVVIVSAGDDLVEPGSPLRDGKDEYE
                     TNSWLLTTAVKEAGAVGYRVHTIPENAAQLKDVIEDQLVRADLLVICGERNDESFSLI
                     YSVLNELGKVREVLPLIEGSGKHAFGVVGPDQTPVVSLPGDPIFAYISAELFIRPMIR
                     NMMGLYDIHRPVIKATLTSDVLSEVGKHSFIRGILSSDKPERRLITPIENQGDLIGLS
                     DATGLIVITEGESGASAGAEVDVLVLERRFN"
     gene            35086..35658
                     /locus_tag="PLW53_02290"
     CDS             35086..35658
                     /locus_tag="PLW53_02290"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD36287.1"
                     /translation="MASIYSHSWPTVLKEDQITLRPLRLRDRSAWLAVRKVNRAWLSQ
                     WEATSPLNHENKNLPSYFEMVNYHRGEGRAGRSLTLAIWFEKNLIGQITLGGISYGAY
                     RGGHIGYWIDERYANKGITTRAVRRLTAYGFEELGLHRIEINLRPENAASKRVAEKAG
                     YVFEGLRPRYLHIDGSWRDHLCYVKENPRI"
     gene            35742..36548
                     /locus_tag="PLW53_02295"
     CDS             35742..36548
                     /locus_tag="PLW53_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36288.1"
                     /translation="MGSGLIYIIIIGMWIAYFLPRWISSHEEISGRSVKRFDEAMKVV
                     GITSGHTAPDIESLNKKREHQIAIRRIQFFSIVGLTIVVSLFTLVGLLSPVILLLPGS
                     VFLMYVVNTRYQISLIKEEVKRVNSANRITSKSSAANYAEIIGRSKRAASLASITEGS
                     GLSEEQWLPLSERLSSYQDSGTITLLPKGSANKDDKSWDPVSVPAPNYANSSKAVPRR
                     VIDLTIPGAWSEAQEKIMREVLAPSADKIFDQEIADQYEEHLRSNRAAGQ"
     gene            complement(36549..37811)
                     /locus_tag="PLW53_02300"
     CDS             complement(36549..37811)
                     /locus_tag="PLW53_02300"
                     /inference="COORDINATES: protein motif:HMM:NF027518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRD36289.1"
                     /translation="MSALMARPILNYRNQIALAIILFFAAITRFANLARPNELVFDEV
                     YYVDGARDFLAYGVESQSGEAEFVVHPPLGKWLIAIGIQTFGDNPFGWRFSAALFGLT
                     SVALIYFITQALFKSNFLSLSATALISLDGLHLVMSRTALLDIFLSFFILLTFYLVIK
                     EKYWQAGITIGLALATKWSALYLLVALCLFLLFYKKTYIKTALQFTILPALTYLITWS
                     GWFLSDIGWKRDSASNSLFSLFNYHREMLNFHTNLKTDHSYEASPWNWLILGRPTSFF
                     YATPKQCGQENCSQEVLALGTPTLWWLGLVSIFITLGYFIYQRELNAGLILLFLFANY
                     LPWIAFPERTTFYFYSIAFEPYLILALIYVMAKALENQELRGVRKRYALVTIGLIGLT
                     FAYFLPLYVGSVLPYQDWYARMWFPSWI"
     gene            37810..38679
                     /gene="rsmI"
                     /locus_tag="PLW53_02305"
     CDS             37810..38679
                     /gene="rsmI"
                     /locus_tag="PLW53_02305"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRD36290.1"
                     /translation="MIDKDTKGHNEAVSELILAGVPLGNIGDASTRLKAAISSADVVL
                     AEDSRRFNRLCKDLGIQCNAEVLSFFEGNESSRLDEVASHLASGRQVLLVSDAGMPSV
                     SDPGYRAVRMAIDKGFKVSVIPGPSAPLAALAISGLASDSFAFDGFVPRSSGARDQFF
                     EDRAMETRTLIVFEAPHRISESLQSMIKSFGPNRQAAICRELTKTYEEVVRGELAELL
                     SWSESKEMLGEFTIVIAPYDPSEQVVSDQEVVETVSRYEASGITRKEAIALTAKELHI
                     RKRKVFDIMVEQK"
     gene            38694..40259
                     /gene="metG"
                     /locus_tag="PLW53_02310"
     CDS             38694..40259
                     /gene="metG"
                     /locus_tag="PLW53_02310"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRD36291.1"
                     /translation="MSDTNKSFYLTTPIYYVNDAPHIGHAYTTVAGDLLTRWHRQRGE
                     SVWFLTGTDEHGQKVMRTAETNNTAPQQWCDRLVEQEWKPVWKHLEIANDDFIRTTEV
                     RHTQRVQKFLQGLMEQGYIYEGKYEGPYCVGCEEFKLPGDLIEGNCKIHSKPVEMLSE
                     TNWFFKLSEFAAPLLEHYKKNPESCQPESARNEVISFLEGGVSDLSVSRSTFDWGVRV
                     PWDEKQVVYVWFDALLNYATAVGLTDDPSSDGGKFFEKTWPADVHLVGKDILRFHAVI
                     WPAMLMAAKLALPKKVFAHGWLLVGGEKMSKSKLTGIAPSDITDHFGVDAFRYYFMRA
                     IPFGSDGSFSWEDMAARYTSELANDFGNLASRLAAMVEKYCDGILPNPAKDEELASAL
                     SKTVESADKAIVALDFQGGINAIMDFCKAVNGYVTIQQPWVVAKDESKKSELDAILYN
                     TAESLRALAILLHPVMPLSTSKLWSYLGAEGSLGPIGSQKISDVAKWGQLKGGSKIIK
                     GDILFPRLPDIES"
     gene            40272..41132
                     /locus_tag="PLW53_02315"
     CDS             40272..41132
                     /locus_tag="PLW53_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRD36292.1"
                     /translation="MADRNNRESDRRAAPLPEPLPTTCVDSHAHLEIVTGAAADHDDV
                     KKVLDDARSVGIDRVVQVGISVKESQWSVDCANYFNTQVLAAVALHPNDAPLIEDLDS
                     DLEEINRLAGEKRVCAIGETGMDFFRTEESLRDKQKYSFKKHIEIAKAHKKALMIHDR
                     DSHRAVLDLLDEVGAPTHTVFHSFSGDADMAKECVAKGYLLSFSGTLTFKNAPLLHEA
                     LMHVPLENLLVETDAPFLTPTPNRGAHNTPAQIPHILRFMSERRGEDLTTLTRAISDN
                     AERVFGSFIR"
     gene            41129..41974
                     /gene="rsmA"
                     /locus_tag="PLW53_02320"
     CDS             41129..41974
                     /gene="rsmA"
                     /locus_tag="PLW53_02320"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRD36293.1"
                     /translation="MSITLLGAGEIRQIAESLDLRPTKSLGQNFVIDSNTCQKIVRLA
                     GITSSDHVVEIGPGLGSLTLALLQASNKVTAIEIDKRLSDQLELTAKSHGASSKNLII
                     VNADAMEVNEIAGRPDKLVANLPYNISVPVLLNFLEKFESITSGIVMVQSEVAERLAA
                     KPGSKIYGVPSAKAAWWADLKLADSVSRSIFWPVPKVDSSLVKFTRHKPLGDERLRKL
                     TFELVDRAFGQRRKMLRSIYADLAGGSAQAEQLLIDSGIEPTIRGEALTIDDFLAIAR
                     QLDRR"
     gene            42001..42915
                     /locus_tag="PLW53_02325"
     CDS             42001..42915
                     /locus_tag="PLW53_02325"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF002870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRD36294.1"
                     /translation="MSAQVLVRVPGKINLQLSVGPLQKDGFHPVATVFQAVSIFDDVK
                     VELNDDGLVRVQSTGKTNNHLPLDKENLAVKAVELMRKKFSISDGITITLTKEIPIAG
                     GMAGGSANAGAAIVGMDSLFNLGLKRNDMEKIGAELGADVPFTISGGTAIGTGKGDQI
                     TPVLSRGTYSWVLALSSSGLSTPAVYSECDRLREGLQVAPPQISDALLQALSSGDSNK
                     LGKALSNDLQAAACSLKPALRLILDVGLDYGALGGIVSGSGPSVAFLAEHEDHALDLV
                     VALTSSGVVGNVVRASGPVPGARVIESL"
     gene            42963..43034
                     /locus_tag="PLW53_02330"
     tRNA            42963..43034
                     /locus_tag="PLW53_02330"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42996..42998,aa:Gln,seq:ttg)
     gene            43026..44528
                     /gene="glmU"
                     /locus_tag="PLW53_02335"
     CDS             43026..44528
                     /gene="glmU"
                     /locus_tag="PLW53_02335"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRD36295.1"
                     /translation="MEEQELSGKEIAQKLNRIGPGAQRGGELNQLDLAILLAAGEGKR
                     MKSSRAKVLHEIAGRSLIAHVIKAIESLAAKELRVVVGADKEDVIQHLNQISPNAKTI
                     YQEVRGGTGHAVKIALAGHNPSGTVLVCAGDTPLLTGATLKDLLKHHVSTGASATVLS
                     ANMPDPSGYGRVLREASGDLAEIIEERDATEIQKQTSEINSGVYIFAASDLVAALGKI
                     GKNNAQQEEYLTDVIGILKSEGKKVVAFETPDFTEILGINDRSQLSQAGFIMNARICD
                     ALMQSGVTITDPMSTWIDITSEIDIDVTIEPGSAIKGSTKIGRAAVIGPRTTLINCEV
                     GTQARVFESHCESSQIGESAQVGPYTHLRSGTVLAEQVRVGSFVEIKNSTIGAASKVP
                     HLSYVGDATIGTDTNIGAATVIVNYDGVEKHQTVIGDHVRIGSDSMLVAPVSIGDGAY
                     TAAGSVITEDVPPGALGIGRARQSNILGWVLKRRKGSKSAEAAAKKEGSK"
     gene            44525..45508
                     /locus_tag="PLW53_02340"
     CDS             44525..45508
                     /locus_tag="PLW53_02340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRD36296.1"
                     /translation="MSNELRLSSEKRLRLFTGRGYPELADEVASELGIPITPTSAYDF
                     ANGEIFIRFEESVRGCDAFVIQSHTAPVNKQIMEQLIMVDALKRASAKRITVIAPFYG
                     YARQDKKHRGREPISARLMADLFKTAGADRLMTVDLHTSQIQGFFDGPVDHLFALPLL
                     ANYVGSKVDRSRLTIVSPDSGRVRVAERWSDLLGGAQIAFIHKTRDPRIPNEAITGRV
                     VGDVSGQTCVVIDDMIDTAGTITKAIDALYDAGAKEVIVAATHPVFSGPAAERLANSR
                     VKEVVVVNTLPIPEESRFPTLTVLSIAPLLGRAIREVFEDGSVTSLFDGHS"
     gene            45495..46859
                     /locus_tag="PLW53_02345"
     CDS             45495..46859
                     /locus_tag="PLW53_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRD36297.1"
                     /translation="MAILKRARLRWLSLLLCAFFLLPLTPAQSNEPYRITVMSRNIYL
                     GADVGVALDLIPNFPAAAQFMWDQMKQTNFKARAPKLANEILQDRPEIIGIQEATSWR
                     CKKDFFGPEVEIYNFLDDLIAATKSAGLSYSIASANGVKAFNPGYSIGAIPYLTKVVD
                     PEVFNPIFGQDSAACGFVIGDALLVRDDVKSQIIQVGNSEYEASYSIVPVLMNIYRGY
                     SWADFKVQDSVVRVITTHLESIWDENKIPNSAIQAQQLVADLKDAKMPIIILGDFNAD
                     YRDPRGVDEPNPGEQPVVSDTCPTSGGAKCNAYSTMIEAGFENASPDAKNARYFTWGA
                     SALLDGPDKKRAKIAKQLGNQYGFTDRLDYIFTKNVYATVSSKIIGNVWPDGSGVWNC
                     GSKICFPSDHAGVVSTIELPRMAGAIDPDLDSHAKSAFTPWYLAAGVILLILIWRIAR
                     RFRR"
     gene            46954..47598
                     /locus_tag="PLW53_02350"
     CDS             46954..47598
                     /locus_tag="PLW53_02350"
                     /inference="COORDINATES: protein motif:HMM:NF004131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRD36298.1"
                     /translation="MAEVSIKGALRSEFGKGASRRIRRDGSVPAVIYGHGEKPIHISL
                     PAREVGVAIKTSNVLLNVDLGEKQELVLPKSIVRNPLKGTLEHIDLIIVRRGERVVVH
                     VPVHTHGKYDPEGILEHVHNTIEVEVDVTAIPASLDLDIEGLMAGDSKTASEVTLPEG
                     VILKSDPKMTVIHLSVRVAFEEVAPVAATPAEGEAAAAAAAPAEGAPAEESKEK"
     gene            47616..48233
                     /gene="pth"
                     /locus_tag="PLW53_02355"
     CDS             47616..48233
                     /gene="pth"
                     /locus_tag="PLW53_02355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRD36299.1"
                     /translation="MFGRSNKDKGGASPNRWLLIGLGNPGKEYEKTRHNIGAALVSES
                     AKKAGVKFSSHKSRADIAEISLGVGAQRVLLIAATLRCYMNESGGPTSSLANFFKVSS
                     DHIIIAHDELDIPFQSIRIKYGGGDNGHNGLKSVTSGLSSSDYYRIRLGIGRPIGEQD
                     PADFVLKPFSSAERKDLDLFLQRGIDAVELLITQGIEKAQNAFNK"
     gene            complement(48234..50924)
                     /gene="ppc"
                     /locus_tag="PLW53_02360"
     CDS             complement(48234..50924)
                     /gene="ppc"
                     /locus_tag="PLW53_02360"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRD36300.1"
                     /translation="MSVPTSAEENLSLRSDVRRLGDLLGQSLARQDGQELLDLVELVR
                     KSVREGGGEDLLQSISADQSVKLVRAFNVYFNLANVAEQVHRSRILAKERIKGGSWLS
                     RAVDNILAAGKSSEGFTSQDIEKWLKNFQVRPVFTAHPTEAARRSVLGKLSTISELLD
                     KSDSPTRDRRLAESVDLLWQTDELRLGRPEPLDEAINALYYLDDLFRLTIPEVLEDFS
                     REISRLGITVSPRDSVLKFGSWIGGDRDGNPNITPEVTKDAIVLQMGHAIRVLNEVMD
                     ELRQALSVSTKIAGTSKQLLDSVAKDLENLPEIEPRFRRINVEEPYRLKATAIGHRLL
                     LTRSRHQNRTEHQAGRDYANTKELIDDLMLMYDSLMQNRGELIAKGLLERTIRTISAF
                     GLTHATMDVREHSQAHANAIQSLFSQTNYLQLSPEEKADFLTHELTQARRDSSKLSEI
                     DGKTLRTFVAIKELQASFDPSVIETYIVSMTKGHEDVLAALYLAKEAGLVDIESGKAD
                     IDVAPLLETVAELRAAGDILEKLLNNKIYRQYVKLRGDIQEVMLGYSDSNKDAGIATS
                     QWEIHQAQRKLRDVAGKYGVKLRLFHGRGGSVGRGGGPTYDAIIALPWGTLDGQIKMT
                     EQGEVISDKYALPALARENVELTLAAALEATILNRSARQSPEDLAKWNSAMESISSAA
                     FSRYRSLVENPELANYFYQSTPVEQLGNLFLGSRPSRRPDASADLSSLRAIPWVFGWT
                     QSRQIVPGWYGVGSGLKAARLAGHSSSMAAMLKDWHFFRTFISNVEMTIAKTDLEMAS
                     KYVLALVDPSLHYFLDDIKAEFELTVNEILLLTGKKDILADQEILAQTLQVRDAYLAP
                     LHLLQISLLKKVREGSSDPLNTRALLLTINGVAAGLRNTG"
     gene            51064..54558
                     /gene="mfd"
                     /locus_tag="PLW53_02365"
     CDS             51064..54558
                     /gene="mfd"
                     /locus_tag="PLW53_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRD36301.1"
                     /translation="MSGKLAGFISLSETTELALEQSDVAVIAADPALPFLIAERSKKA
                     PQLVITHSSSRASEIALQLSELVDQVAEFPAWESLPHERLSPNSDTVARRIDTLINLD
                     KTKVVVTTARALLQPINQEIIEMPMLSIQSGRHQNFSELIQELTHRAYNRVDMVERRG
                     DFAVRGGIIDLFPPLADHPVRIEFFGDEIEEIKYFEVSDQRTFALVDGSLNLIPCREL
                     ILTPEVAERAQKLAIKYPEITEICNKAAEGIYSQGLESLLSVLSKKLVPLLELLPKGY
                     EVISLDQERIALRVRDLISTNEEFLEAAWSSAALSQGDDESFNTPLRQELSTGGFLEL
                     GEVTSYAADNGIIWRYINSYGTPEDLQINQFIAVEPFKNNFEKLIQQVKTWISQGFLV
                     VISLEGIGILERYRDIFADGDIAVALVEKLSSDLLPDKLYLTSTVMRHGFIDQEQKIV
                     FLTEADITGNKDLRAATSRMPSKRKASIDPLELKSGDYVVHEQHGVGRYLELVQRDVA
                     GISREYLVIEYASSKKGHPADRIYVPTDSLEQITRYIGGEAPAVHRIGGGEWIKAKSR
                     AKKAVKEIAGELIRLYAARTSSPGFAFSPDTTWQRELEDSFAYVETPDQLVTINEVKE
                     DMQRPYPMDRIICGDVGYGKTEIAIRAAFKAVQDAKQVAVLVPTTLLAQQHLATFTQR
                     YSGFPITVSALSRFASSKEISETLAGLASGGVDIVIGTHRLLSDDVAFRDLGLIIVDE
                     EQRFGVEHKEKLKKLRASVDVLAMSATPIPRTLEMAITGIREMSTITTPPEQRHPVLT
                     YVGAYDEKQVAAAIHRELLRDGQVFYIHNRVESIDDVAAKIRRLVPQASVAIAHGQMS
                     EANLEQVVVGFWNREFDVLVCTTIVENGIDVANANTLIVERADLFGLSQLHQLRGRVG
                     RSRERAYAYFFYPIDRAMSEVALDRLSTIAQNTELGAGMRVALKDLEIRGAGNLLGGE
                     QSGHIADVGFDLYMRMVGDAVNEYKAGFIDGGQELTDCKVELPVTAHLSAEYVPSDRL
                     RLDLYRRLADTKDEDEITKIGEELSDRFGALPKEAENLLRIARLRTYLKERKIQDFAV
                     QGRYVKIAPLLPSESLELKIQRLYPGSIVKSVTQVVMIARPQTAAWVSEAEEIGDTSL
                     IDWAVELAKTLLERPLGK"
     gene            54566..55165
                     /locus_tag="PLW53_02370"
     CDS             54566..55165
                     /locus_tag="PLW53_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36302.1"
                     /translation="MKKFLAPIIAILALALSSCSQVDSAATIGDEKITIAALQEQVDL
                     ILAEREGIDTTQMSLESGEALTRSQLSYMISNLIINEVAKEEKIEIDQAEIDAYKIEI
                     YANIGGEENLPNVLVSASIPSTSLDNVLRRDLILRKITDGAIASGVSEAEVNDLIQKI
                     VTDKATALSVEVNPRYGTWDATSLTVVAKEPAGDAVNDK"
     gene            55181..55822
                     /locus_tag="PLW53_02375"
     CDS             55181..55822
                     /locus_tag="PLW53_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HRD36303.1"
                     /translation="MAYENLARLAQVMDTLRSPGGCPWDSEQSHQSLLKYLLEESYEF
                     IEAVESGNREDMREELGDILLQVYFHSRIAEEHEEKPFNIDDVAKAIIEKLISRHPHV
                     FSDAKVDSSAEVLENWEEIKRKEKSRSSAHDGVPTGQPSLALASKLIYRASKNDLSTP
                     EHPKEKIEVSEAALGNQLLSLISWAIANNLDPEVALRKAALKYRDAMSEEESG"
     gene            55834..57123
                     /gene="eno"
                     /locus_tag="PLW53_02380"
     CDS             55834..57123
                     /gene="eno"
                     /locus_tag="PLW53_02380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD36304.1"
                     /translation="MSTNSAIKSITAREILDSRGNPTVEVDVQLEDGSWARAAVPSGA
                     STGAFEAAELRDGGSRYLGKSVSGAVENVRTKIAPALIGQDGLDQKGLDEKMIALDAT
                     VNKSSLGANAILGVSLAVARAAAVSKKLPFYKFIGGEKATLLPIPMMNILNGGAHADT
                     NVDIQEFMIAPIGAPSFKEGLRYGAEIYQSLKSVLKKRGLATSIGDEGGFAPNLESNR
                     AALDLIIEAVEKAGYKMGSDIGLAMDVAATEFYKDGSYHFEGSNLSATEMINYYQGLL
                     DSYPLLSIEDPLSEDDWSGWADITKAMGASVQLVGDDLFVTNPTRLAQGISQGCANAL
                     LVKVNQIGTLTETLAAVDMAHKAGYRSMMSHRSGETEDTTIADLSVAALCGQIKTGAP
                     ARSERVAKYNQLLRIEEELGSKAQYAGRDAFPRFTSR"
     gene            57154..57600
                     /locus_tag="PLW53_02385"
     CDS             57154..57600
                     /locus_tag="PLW53_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRD36305.1"
                     /translation="MKRSKISLPKTLESFAAKRGISTRLLILFLVLFIVAVTLAPPTQ
                     QYFAQRAQISALEADLDSNYKKLEAARAELQRWRDPDYVKSEARNRLHFVLPGERQYI
                     ILGAKPTIDPEAQGAAPINQDFPLGVPWYSRVISSITSVGVGVTTP"
     gene            57610..58116
                     /locus_tag="PLW53_02390"
     CDS             57610..58116
                     /locus_tag="PLW53_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HRD36306.1"
                     /translation="MGERASDEDLSAIEIQLGRVPRDVHSIAYRCACSHPAVVETNPR
                     LSDGTPFPTFYYATCPNLTAAISTLENSSLMEEMNERLAIDAELSGQYRAAHDDYLAA
                     RAALGIDVPEIVDISAGGMPDRVKCLHSLIAHSLAAGEGVNPLGDEALSLLPKWWLNG
                     NCLERRDQ"
     gene            58113..59027
                     /locus_tag="PLW53_02395"
     CDS             58113..59027
                     /locus_tag="PLW53_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRD36307.1"
                     /translation="MSRVAAIDCGTNSIRLLIADVSGSNLREIYRGMEIVRLGQGVDK
                     NKSFDPDAIDRTLRATELFASEIRRRGVESIRFCATSATRDASNRELFIDGVKAILGI
                     EPEVISGEQEAELSFIGATHQLNQIGAPYLVVDIGGGSTEFVYGQSTVEASVSENIGC
                     VRMSERHLNSSPPSKAAIDLAITDIDSAIKNAATKVPIASAKTLVAVAGTATTVAAAA
                     LGLESYDRDLIHLSRISAEKTHQVCQSFLALERSEIASLGYMHPGRVDVIAAGSLVLS
                     RIMALSGASEFVASESDILDGMALLLAK"
     gene            58999..59787
                     /locus_tag="PLW53_02400"
     CDS             58999..59787
                     /locus_tag="PLW53_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRD36308.1"
                     /translation="MEWLFFLRSKPRVIAKDIKSLKVLDKEIIKCSACPRLTSWRKEV
                     AITKRAAYRNEDYWGKPVTGFGPSNSKLLIVGLAPGAHGANRTGRVFTGDSSGDWLYR
                     SLHKNGLAKISTSLSSDDGQELFETRITCALRCAPPGNKPTTIEQQTCKSWLETEMKL
                     LMPTLRVFLPLGQIAWKATIDALLAIGEELPRKRPKFGHGASFNFLGRDGNTRLVIGS
                     YHPSQQNTFTGKLTRSQLDSVVKKAGRFAHAEVAPVAQRQRQTT"
     gene            59752..59824
                     /locus_tag="PLW53_02405"
     tRNA            59752..59824
                     /locus_tag="PLW53_02405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59785..59787,aa:Leu,seq:taa)
     gene            59879..60673
                     /locus_tag="PLW53_02410"
     CDS             59879..60673
                     /locus_tag="PLW53_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRD36309.1"
                     /translation="MNSSNPVLGKAFGEKGRRGYAAMDNFPQDNLEQSFNAPAASSLR
                     TGRMTMDDVVARSGMLFGTSALVGAAAWYFNLGGGLLLLGFVGGLVTAMIGIFSKTIR
                     PGVYLAYAVCQGLVLGIISKTYELFYPGIVQQAIVATAAAFIGMLTLYKSGRLRVTPK
                     FTRMLLGAAIGYLVLALGSLIGSFFGLGGGAGLYGLSGFGPLLAVAGVAIASFFLILD
                     FDQIEEGVRAGVPQEESWRAGFGLLMTMVWLYLEVLRLISILRGND"
     gene            complement(60674..>60936)
                     /locus_tag="PLW53_02415"
     CDS             complement(60674..>60936)
                     /locus_tag="PLW53_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36310.1"
                     /translation="ASAGAIVGDVIKGKSGKVYAFWQMAGDAGMMVGPVLLGFISDAF
                     SYRTAIGVSAAVFSLAIIVALKIPETNSAHLGDSRKPPINEV"
BASE COUNT        17437 a        12596 c        13712 g        17191 t
ORIGIN      
        1 gcgatgttgt tctgctgcca ggtgctggag ctctacttgc acctgattgg attccttata
       61 gttcaagaat taccgcagga gatgttggag tgggaacaat tgttccaaca gctcttgatg
      121 atccacgttt agttcctgca gctagcgcgc taccaggtga tgaagaatta gatctgcatg
      181 aattatttga atttggcgca ggaagaaatc gcattctctc tattgaagga cgcgatcaag
      241 cagcaaagcg ttggataagc ggagaccgcg gtcctgatac tccgatggcg caatttgctc
      301 caaagaattg tggaacttgc ggcttctatc tacctatcgc tggctcattt agggcggcct
      361 ttggcgtctg tgccaatgcc atctcccctg aggatgcacg cgtagttgcg gtaaatcatg
      421 gatgcggcgc ccattcagag gcgataaact agagccctgt ccgcagagcg cggcgcacat
      481 gacacctaaa aagaatttat aaataaggag gttgctctca tgccagttgg acgcgtgaaa
      541 tggtttagct tggagaaagg ctttggcttt atctcatctg atgagggcga agatgtttat
      601 cttgcagcct cagcacttcc tgaaggtgtt agcacggtta agcctggaac taagttggaa
      661 tttggtgttg cagaaggacg caaaggacct caagctttgt cggttcgagt tattgaagcg
      721 ccaccatctc ttgctaattc aaaggctgga gcagatgatt tagcggtaat cattgaagaa
      781 tcaatcaaga tgctagatcg agttggtaat ggtctgcgcc atggtcgata tccaagtgat
      841 gcagaggctg ctcgcgttgc taaagtcttg cgtggtattg ctcaagaaat tgaaggctaa
      901 cgagcctttt tctctctagc ttttgtgtag tagataccaa taaagcccag aagtattgca
      961 agtaggcagg taagaattat ctttgctact gcatttacta ctattgcaac aactaaagca
     1021 atactccata aaacgattcc aaagatgatt actctaattg cgctctgcat atcagcaatc
     1081 atactagaat acgcagatgg gaacgctgcg atcatttaag catcgcaact tcaagattct
     1141 cttcagtgca aactttctct caaatatcgg cacctgggct caaggcgttg ctcaagcttg
     1201 gttgatcctt gagctaaccg atagcggaac ctatcttgga attgtgactt ccttgcaatt
     1261 tgctccaata cttttcttta gtatctctgg tggaaagatt gcagataagt tcaataagcg
     1321 aaaagttcta atgcttacca acttaactgc tggaatttca gcgctctcgg ttgccacctt
     1381 ggtgcttaca gaaaatatca agatctggca tgtgatgttc tttgccttca tgcttgggat
     1441 gggaaatgcc atcgatgctc cggttcgcca gagctttaat gttgaagttg ttggtaagca
     1501 agatctacca aatgcagtag ggcttaactc aacaaacttt aatattggtc gcttgattgg
     1561 cccaggttta tcaggtttat tgattgctgc ctatggcact ggggtctcat tcctgctcaa
     1621 tggaatttcg tacttagttg taattattgc gctgattaat attcgtgaga gtgaactatt
     1681 cattgaggag aagaaatcca gctcaaccaa gataagagag gcgatggctt atgttgccgc
     1741 tcgcccagat attttggcag taatgatcac agtatttttt gcaactagct ttggtcttaa
     1801 cttcaatatt ttcaacacca tgatggccac tcaagtcttt gataaaggcg cagcagagta
     1861 tggggcgctt ggaagtatct tggcagttgg atcactttct ggggcaataa tctctgcgcg
     1921 acttgagaaa aagcgtggcc ctagatttgt gatgattggg tcgatgatct ttagcgcaat
     1981 cttaattctc tctgctttcg caccgactta tttagtttat tcaatcttgc ttccaattat
     2041 tggttgtgtg gcgctactta cctttattgg cgctaatacg atggttcagc taagaactga
     2101 ttcgcagata cgtggcagag tgatgggaat ttatctcaca gtgttcttag gaggaactcc
     2161 tatagtttcg ccatttattg gaattatgac tcaggaagtt ggaacaaggc caacagttgc
     2221 aatctgcggg ctaatttcac tacttgcagc aattgccacc tttgcaaagt ttaaagataa
     2281 gggccaggag ccagatagct ttgctattgc agatgtctta gagcccactt atgagaacaa
     2341 aaaagactaa gctaaaaagt attaccaggg taacaattct ccgcgtcttg taattcatca
     2401 tttatcctta cttttttagg ttttcaacca catattcgat gcatttagtt agagccttaa
     2461 tatcctctgg atcaattgct gggaacattc caactcgtag ctgattcttg cctagtttgc
     2521 gatatggctc ggtatctaaa attccatttg ctcgaaggac tttagcaatt tctagtgcgt
     2581 caatatcatc tgagaaattt atcgttgcaa caacattaga gcgcattgct ggatcagtta
     2641 caaatggggt ggtgtaggaa gttttttctg cccagctata aatgatttcg gcagactttg
     2701 cagatctacc agcagcaaat gccattccgc caccttggtt catccactca atttgttcag
     2761 caagcaagat tagagtggca actgctggag tgttgtaggt ctgatcaaga cgtgagttat
     2821 caatagcgat agttaaatca aagaatgctg gaacccatct tccagagctt ttaatctttg
     2881 ccactcgttc aatagcagca gggctcatta gagagatcca gagcccacca tctgaggcaa
     2941 aagatttctg cggagcaaag taataggcat caaattcact aggagagacg ctcaggccac
     3001 ctgctgcgct tgtggcatca actaaaacta aagcgccatc actttttgct gggcgcttaa
     3061 tctgcatcgc aacaccagtt gaggtttcat tatgggttag gcagtaagca tcgatccctg
     3121 cttcaacaac tgcctgcgga tgagttcctg gttcagattt aataacactt ggctcatcaa
     3181 gaaatggcgc ctcttttgca gcttgagcaa acttagatga gaactcacca aatgataagt
     3241 gctgcgattt cttttcaatt agaccaaagg ttgcgatatc ccaaaaggca gtagagccgc
     3301 catttccaag cactacttca tatccttgtg gcagatcaaa gagtgagcta aggccacttc
     3361 taacgcgtga aacaacgctt ttaacttcct tctggcgatg agaggtgcca agaatttttg
     3421 cgccaggtcc tgctaaaact tgaagtgcgg cagggcgaat ctttgatgga ccacaaccaa
     3481 agcgcggatc actgggcttt agattctccg gaatttttat ctcagacatg attgccaaat
     3541 cctatgggga gagtcgctca aggctccaat tggaaccact taaattaaag cgaagtcgat
     3601 catgcaggcg tgaatatcgc ccttgccaga attcaatact gtttggctta actagatagc
     3661 caccccaatt ctctggcttt ggcacgacgc tgccttgcgg gtatttctca gccaattctc
     3721 taaagcgctc ctctaagacc tgtcttgatt cgataacctc tgattggcta ctagcaagtg
     3781 cgccaatctg actagaccag ggacgagtag caaagtattt ctctgactct gaactgtcta
     3841 tcttgcttgc acttcctata actattacct ggcgctccat tggataccaa ggaaagagaa
     3901 gtgagacctt tggatttgct tggatctgac gagatttatc tgattgataa ttggtaaaga
     3961 aggtaaagcc attttgatca acatctttta aaagtactgt tcgtgagctt ggcccgtctt
     4021 ggccaagagt tgatagcacc atggcattgg cctcaatgat catggaattt gctgcagcct
     4081 cttttaacca gagagaaaat tgcgcaattg gatcaggatt tattgagctt tcagtaattc
     4141 caacctcgcc atatgagcgg cgcatcgctg atattgcctc acggcttatc tctgggctct
     4201 ctgccatagg tgtatctaac gtcactttgc cagccttggc atctttgcct tgcctcattt
     4261 ggcccagcgg ggtattaagt ggagaatacg gcgcatgaat gaagacttta agccgggcct
     4321 tgaaggggta atcgcttttg aatcagagat tgcagagccg gataaagagg gaagtgcgct
     4381 gcgatatcgc ggcgtagaca ttgatgatct agttggtcga gtcacctttg gcaatgtctg
     4441 gggcttacta gttgatgatg aatttaatcc tggtcttcca gcagcagaac catttccaat
     4501 tccaattcac tcaggtgatg taagagttga tgtgcaatca gcaattgcaa tgcttgctcc
     4561 agcttgggga cttaagccac ttctagatat ttcagatgaa gaggctagaa gtaatctagc
     4621 tagagcttca gttatggtgc tttcatatgt ggcgcaatct gcccgcggaa cttggcagcc
     4681 aatggtttct caagcagaag tagataaagc acataccgtc gttgagcgaa tgatgattcg
     4741 ctggcgagga gagccagatc caaaacatgt tcgcgcaatt gatgcctact ttgtctctgc
     4801 agcagagcat ggcatgaatg cctcgacatt tactgcaaga gtaattgcat caacaggtgc
     4861 tgatgttgca gctgctttat ctggagcaat tggagcaatg tctggcccac ttcatggcgg
     4921 cgctccttca agagttttgc atatgattga agaagttgaa agacgcggag atgcaagagc
     4981 gtatgtaaaa gagttgttag ataaaggtga gcgcttgatg ggctttggcc atcgcgttta
     5041 tcgcgcgcaa gatccaagat ctagaactct tcgtagaact tgtgaagaac ttggcgcacc
     5101 tcgttatcaa gttgctaaag cgctagaaga tgcagcgtta gcagaattac gcgagagaag
     5161 accagataga gttttggaga caaacgttga attctgggcc gcggttcttc ttgattttgc
     5221 tgaagttcca ggacatatgt tcacgccaat gtttacttca gcaagaactg ctggatggtc
     5281 tgcccatatc ttggagcaga agagaacagg tcgcttaatt aggccatctg ctcgatatat
     5341 cggtaaggcg ccaagaaggc ctgaggatgt taagggctgg gatgagagcg tttcaggact
     5401 tcacctctag tctgaaaact agcgtgtaaa ggcaagtcct ttataacaaa taagttaccc
     5461 ctcaagatag cttgccgata gggccctttc gtgttgacgc catctttgtt tagggctgga
     5521 attgccttct ctgcagcccc tcgctgtaga tacttccatg gaggaaaaat taatgaagaa
     5581 gaaattcttt agagggttcg cagtactagc aacacttgct cttgcagcag gccttgctgt
     5641 gacacctgca cacgccaaca agaatattgt ggtctgggca gatgaatcac gcggaccaaa
     5701 tcttacaaag acactagcaa caaaaggtga ctgggttccg ggatataccg taactgtaaa
     5761 gtcatttgct aacttcgatg cgctgaaaga tgcgatcgat aaggcaacag caactactgg
     5821 tccagatatc gtagttggtg ctaatgactg ggttccaaca cttgctaaga atggaaagct
     5881 agctccatta actctgaccg cttcacagcg cgccagattc tcagcatccc agttctatga
     5941 tctttcttat aagaagaagt tgtatggcat tcctgttgac gtaaacaacg tggcaatgat
     6001 ctacaacacc aagcttgtaa cttccgctcc aaagtcattt ggtgaaatgg ttgattacta
     6061 caaggccaat aagacttcca agagcctaaa ggctggtcta tgtgtagccg gtggcggtat
     6121 gtcatggggc gcacactcag tattttcagc acttggagct gatgcttatt acttcaatcc
     6181 aaatggaact ataaataagt ccaagtcatt taatccaaat gagtttggcg ctaatgtgaa
     6241 gaagttccta ttagatggca agaagagcaa tggtttcttc ccagcaacag atactggttg
     6301 caaggacaac ttcctagccg gaagcgttcc tttcgcaatc attggaaact gggagtggaa
     6361 cgattacaag gcaaaaggat tcactatgaa cctaatgcca gttccaggtg catcctcagg
     6421 taaaagtgga aatgcattcg gatcagtatc tggtgcactt ctaacaacat tcgcagcatc
     6481 aaatggcgta gaggcagcag ctaagtctct acttgttgat ttcttcggat ccactgcagg
     6541 tcaggttgcc tatcagttaa atgaaaagcg cccaccagca gaaaaaggtg ctgcttccga
     6601 tgcaacagtc acagatggcc aaaaaggatt tggtgcctct gctgctgctg caagcattcc
     6661 tcaggttggt gctattttga acggacctag tggtaagagc tactgggatt cagcacctgc
     6721 ttactggact gcagttctag tcgatggcaa ggacgctgta actgaagcta agaagttagc
     6781 cgcaatttgg cgagcaaacc tcaaggctgc ttataacgac ctatagtcaa cagaaaacct
     6841 cttgattgat atcaagatga attaggtgaa ctgacgtggt tctaagggac cacgtcagtt
     6901 cgctttctgc actaaagttt caacgaacta tttggagttg gtgatcaatc tgctttcgat
     6961 gcttatccga ggaaaagttg caacctttat aaaagttgca attgtggcgc ttatcagcgc
     7021 tttactcctt actctgacac agagcgcatt tgccaaaaaa gagtatgtca tcgcagcatt
     7081 tcttgccgtt gctatttttg caattaattt cacctacttt aacaagcgaa ctgttccgct
     7141 taaattcttc ttaccaggta ttttgctctt atcagcattt gttgttactc caattcttta
     7201 taccgttgtt atgtcgacct ataattacaa gactggaaac tacatcagta aagaagaggc
     7261 gattggtcgt atcaaagcgc ttgctcttga accagatgag aataattcag cttttgatat
     7321 tgtggttgga aaattccaag aaagtgaagc aattctagtt tcagatatag aaaacaaatc
     7381 gtatttcatt tcaacaccag agcgcagatt agaacttcaa attgatcaag tagaactcga
     7441 tgaaaatttc gttgcgcaga gagccaacgg atttactccg cttgaagcag gcgaaattgc
     7501 ttcaagcaaa gcggcgacga tccgctattt ttacaactcg caatacttta ttactgtgga
     7561 aaatctcaac gtcggagcag tatttcgcca gagcctggag tatctgccga atcaagatat
     7621 ttttaagagt ttaataaatg attcaatcta tcgcgacaat ggtaatggaa attacgttaa
     7681 tccagaagat aaatcggatt ttcttgaacc aggatggaga gatccagcgt ggtttaccaa
     7741 ttacataaga ctagttagtg atgagagggt tagagatcca ttaatttcag tctttatttg
     7801 gaccgtagtg tttgctacct taactgtgct tacacaattc tccttcggcc tacttttagc
     7861 tctagccctt aataagccca ttcgtggtcg ccgcatatac aggtcaatct tggtgcttcc
     7921 ctatgcaatg ccaagtgtca tgtcaattct tatttgggga ggaatgttta atacagaatt
     7981 tggtgcggtg aatgaaattc taggaagtga cattgcctgg tttcaagatc ctacatttgc
     8041 tcgatttgct gtgattttag taaatctatg gctgggattt ccttacttct acttagtttc
     8101 gagtggatcg ctacaggcaa ttcctagtga gttattggag gcggcagcga tagatggggc
     8161 aaatccaaga caagttttta gcaaagttac tctgccttta cttcttcaaa ttctctcacc
     8221 tttacttatt gcatcttttg cctttaattt taataacttt aatctaatct acctgttaac
     8281 tggcgggggg ccaagagagc aattagatgg tgagattgct gggtctactg atattttgat
     8341 tagctatacc tatcaaatag cttttggaag taatctgcaa gatttaggct tggctagtgc
     8401 aatatctgtt ctaatcttct tcttggttgc cagcatttca ctttatggag tacgaaaatc
     8461 aaaagttttg gagagtttta catgacaaaa tggtttagag aaaacctttg gcgacacata
     8521 actggaacag ttatgtgctt gttcgcactc tttccactct atttagtcct gttatcggca
     8581 ttcaatgagt cgggaagtct tcaagtaacc tcgtttttac cgcgtgaaat ctcatttgcc
     8641 aattttgatg tgctttttaa cgatgagcga atcccatact taacttggat gaagaactca
     8701 ttgattattt caggttttgt ggcagtggct tcggtagtta ttggagcggc ctccgcattt
     8761 gcttttagcc gcctaaaatt tcaagggaag aaatttggta ttcaattact tcttttgatt
     8821 caaatgtttc ctgccattct ggcgctatct gctttgtatg tgattatgga acgtgtttat
     8881 aaatttgctc ctggcctggg aattggaacg caactgggat tggttttggt ctatctcgga
     8941 ggagccatgg gggtaaatat ctggctatta aagggcttcg ttgattcaat ccctgcggaa
     9001 ttagatgaag cggccaagat cgatggtgct agcgctatgc agacttattg gcagatattt
     9061 gtgccattgg cagcaccagt cttagcagtt gtctctctct taagctttat tggaacattt
     9121 aatgaattta ttttagctag attgttcttg attgaagtag agagccgaac agttgctgtt
     9181 gggttgcaac aatttattgg cggccaatat tctgagaatt gggggccttt tgcggctgga
     9241 tcgataattg cttccatccc aattgtgatt attttcttaa gcctgcagaa atacattatt
     9301 agtggcttaa ctgctggttc tgttaagggc taatgaagct ttcaaggctg gccttatttg
     9361 cactacttgc tttactgctt gtgccattta catcgcttca tgcccaaact ccaaaggtag
     9421 atattgcaag aaccggtata gataaagagg ttatttactt tgttatgcca gatcgctatc
     9481 gaaatggtga tgtaagcaat gatgacttgc caggatttaa tccaactcat actgcttttt
     9541 ttcatggtgg agatttaaag ggtcttaccg gtaactgtgt tgatgatgat ggcctagcaa
     9601 gattaaagaa attaggattt actgccattt ggcttacgcc tttagtggtt caacaaccac
     9661 caactgatgg tggcgctgga tatcacggct attggggcgt tgattttctt ggcgttgatc
     9721 ctcacttagg cagcaaagaa gacttaatgg cgcttcgaaa atgtgccgag aagttgggat
     9781 taaaactaat tcttgatgta gttaccaatc acaccggaga cgtagtttgg tataacaatc
     9841 gagatgctta tattccagat aagtataaaa atataaagaa tccaagttgg cttaatgaga
     9901 taagtaatta tcataactat ggcgatatga atagttgctg gagccctggg gcatgcgtca
     9961 gagatggtga tttttttggg ttagatgatc tagctaccga aaaacccgag gtatataacg
    10021 gacttgctgg aatctatagc gattggattg aaaaatatgg atttgttggc tttcgcgttg
    10081 atactgccag gcatctagat gatcagttct tcaaaaactg gagcccacag ataaatcaga
    10141 ttgcagaaaa atcaggcatt gccaacttca caatctttgg tgaagtttgg gagccaagcc
    10201 caattgccct gatgccatat atcagagtta ataaaatgca aagcgctcta gattttccgt
    10261 ttcaaagagt tgctgttgat tacgctgcct ctagttctaa cgcttcaatc ttaaagaatc
    10321 tctttgccta tgatgattat tacaccagcg caactacctc agcttcaaat ctagtcacat
    10381 tccttggcaa tcacgatatg ggacgagcaa attttctaat tgaacgcgtg aaggtaaatc
    10441 caccagggca acttctctct cgagtaaaac tggccaatac tctgctctat ctaagtcgtg
    10501 gagttccaac ggtttactac ggggacgaag tcggaatcct cggaagtggc aatggtagag
    10561 atcaattagc tcgccaagat ttgtttccaa ctaaggttga aatttggaag caagagccca
    10621 gagttactgg taaaccagtt ggtagcgctg attccttcgt cgaaaccttc tcaaatccaa
    10681 ttgccaagca tctaatcact ttatctgaac tgagaaaagc ccatccggcg ttagcaaacg
    10741 agcagatgca gattcgctat gccaagggct cagtttttgc attttcaaag cgagcaatta
    10801 gtgagaagag agagtatctg gtagttctaa acaatagcgc caagaccagc acggtaaaaa
    10861 tctcaacagc taccagtgga aaatggaaag atttaatcag tggtaaatcg tttacgagta
    10921 agagcggtga gttaactctt aaggtagatg gacttagttc agctgtgtta agagcggaaa
    10981 gtgagataaa ccagagagga ataaagcttg gaaaactctc cgcaaaagag gactttctaa
    11041 gtggtttcta taacatttcc ttaaaagttg agagcaaaga tctagcaata gcagagttct
    11101 ttattagaac aaaagctgat agcaaatgga gcactcttgg tgttgacctt aatcaacctt
    11161 ttaatgtctt tatcgatcct aacgaatact ctggcccgat tgaagtaaag gcaaatgtta
    11221 ttgactctaa aggagggaaa aatgagcttc gagaaatcgc tttcaatatt gccacaccat
    11281 gatggcagtg aactttactt aagctcaagc tcgcccacat tacaggagag agttaccttt
    11341 aagtttcgtg caggttctaa tttcccaatc gagcaagcaa tattaaggct ctatcatgat
    11401 ggagaaccac gtttctttcc aatgaagcga agcattatta agggtgagca gtggtggcaa
    11461 gttaaggttg agataaggaa tttgaaaacc ccttatcgat tcttaattgt aaatggggat
    11521 acatactcct ggcttaatgc tcgcggcttt cagagccatg atgtaactag cgcaaatgac
    11581 tttcaattac tagcaacacc agcttttcca aaatggattc gaagtgctgt tttctatcaa
    11641 atctttcccg atagatttgc aacaacagga aagtatcagc acttaaagcc tgaaaaattc
    11701 atttcaaggc cttgggatca gcttcctaaa ggacgagata agagtacggg agtggagttc
    11761 tttggcggtg atctcgatgg agtgtctcaa catctgaagt atttgaaaaa attaggaatt
    11821 aatggaattt atttcacacc aattttccct gctggctcaa ctcatagata tgacgcaagt
    11881 tccttcgatg aagtcgatcc gcttcttgga ggtgatgctg cgctaatacg tctagcaaag
    11941 aaggcaaaga gtgcaggaat ttatttaatg ggggatttaa ccactaatca ttgcggcaga
    12001 gagcaccgct ggttgcaacg atcgttgaaa tcaaagaagg ctaaagaaag agagtttttt
    12061 tattgggata agtcggtaaa gcatggttat gaggggtggt gggggcttgc ctctcttccc
    12121 aagttaaatt acaactcaaa aaaacttcgt gagttgatgt atgaaggtaa gaattcaata
    12181 gttaagaaat ggctgaagaa accctacaac ctctccggat ggcgaataga tgttggcaat
    12241 atgactggtc gctacaaagg agaagacatc aacggcgagg ttattgctgg cgtgaggaaa
    12301 gcgctgcagg agagtaatag ccaagcttgg ttagttgcag aaaatgcaga ccatttcccc
    12361 agtgatttag atggatttgg ctggcatgga actatgaact acaacggctt tatgcgtcct
    12421 atctggggat ggctaaacca tagagccgaa gttgagaaag ctttctttgg tgtcccagta
    12481 tcaattcctc gttttactgc tggcgaaatg gtttcggcaa tgaaagagtt ttcttcaact
    12541 attccttgga gaaattttgt ctcctcaatg ctcttacttg attcacatga cactgcaaga
    12601 tttagaaatg ttgtagggaa agatactaag cgccatattg ctggcatggg tcttctactt
    12661 acctatcctg gcgttccctc aatttatgca ggcgatgaat taggtgtaga gggacagtgg
    12721 ggagaagatg ggcgcagaac tatcgattgg agcagacaga gctgggatca tgaatttcta
    12781 agcgaagtta gtaagctgat taaaattaga cgccagtctc acgccttagc tcaaggaggg
    12841 ctgcgttgga tattgattga agatgaccta ctggcctttg aaagagaatc taagcgggaa
    12901 aaacttttag tagtagtgag ccgatctgct cagaagtgca aaattgaagg catcggcgaa
    12961 gtgaagcagc gcctatatgg ccctgaacta aaggggcaga tttataaatc tgacagtgcc
    13021 tctcttggca tttatcgcct cttttaatca agatcgcagc gctaaatatg aaaagtagtg
    13081 gttgaatcac gccatgagta agcgctctat tttctggttt cgaagagact taagacttgc
    13141 cgataatcct gcgcttctag cctgtcttga tgaagcagat gaagttgtgc cgctatttat
    13201 tttggatgat gaaatctcag aacgagccgg cgatcatcgc agagcttttc ttgctaattc
    13261 actaagggct ttaaatgatt ctctaggagg cgctcttcat gtgattagtg gaaatccagt
    13321 ggaagtccta tcaaatttaa tgaagcgata tgaaacaacg tcaattcact cagcttttcc
    13381 ctttgctcca tatgggaatg caagagatga agagctaaga agtgcaggga tcaaattaga
    13441 acaacttgga tcctcatacg ccgttgctcc aggaagagtt ttaaaaggtg atggaacgcc
    13501 ttatcgggtt tatacccctt tttatcgagc atggttagct catggttggc gcgctccagt
    13561 tgcagcacct aaaagtccta attgggtcac accaaagaaa agtgatcaga atattccaaa
    13621 ttggaaagcc cctgaaggaa ttgttttaca agaagcggga gaagcagctg cgctcacaag
    13681 atttaagaag tttttaaaag attctgctgc agattatggc gataatcgaa atattccagg
    13741 cattgaagga acaagtaagt taagtccgca tcttcgttgg ggcgagattc atccccgaac
    13801 tattcttgcc gcacttggtc agtcaaaagg ccatgaggtt tatcgcaaag agatagcttg
    13861 gcgagagttt tatgccgatg tacttcacaa taatcctcac actagccgcg attactacga
    13921 tcccaaatac aaaaaaatgc gctatgaaaa acctggaaag gcatttgaag cctgggctag
    13981 cggaaagact ggttatccat ttgttgatgc agccatgcgc caattagtta aagaaggatg
    14041 gatgcataat cgaactcgca tggtggtggc atcattttta gtaaaagacc ttcacattga
    14101 gtggcaacat ggcgccaatt tctttatgaa atacctaatt gacaatgatg tggcatcaaa
    14161 ttcacatggc tggcagtgga ctgcgggatg cggcactgat gcctcgcctt attaccgtgt
    14221 ctttaatcca attgagcagg gaagaagatt tgatcctgaa ggaatttata ttaaaaaata
    14281 catcccagag ctctctcatt tgcagggcga tgaggttcat gagccttggg agtccaaaga
    14341 tggatataag aatggctatc caaaacgcat tgttgatcat gcagcagaaa ggcttgagtc
    14401 actggctcgc cttgaagaga tcaaggcagc taagccactc aagccttaat tgctaaacca
    14461 ggaagtttga gaactgccaa ggatcgctgc tatcaacttt cttgccattc cagccttcaa
    14521 ataaatcttt tcgattcatc aaaggatccc aagcgcttgc ctcggagtga ataccgcccc
    14581 agtacttctc ggcatagcta agtacttcgc gccatggaag ttcatccggc acaagtaatc
    14641 ctttatcagg atttttcatt gcccaagcaa ctgctgcata aacggcagag gcaacttgaa
    14701 ctgttgttgc tgattgatca ggaatcaact ttcttgattc atggatgcta agcagtgatc
    14761 ctgtccacca agctttatag ggatggccca ttagtaatac tccaagacga tcatcaccat
    14821 ctgtgatctc atcattcata attctctgat ttttatgaag atcccaagag cgcatctcaa
    14881 gttccttcat cgaagcaata gcttcatttg tggggcagta ggcatagtga accgttgggc
    14941 gatagagagc cttggctcca tcccagacag ttaagtgatc tgagattgta aatgcctcac
    15001 catggcgaat taccatgcca tggatttcaa agtttggcac ccatgagcga acccaggttg
    15061 ttgctccagg ttgagcaatt gcaatttgat tctttggtcc actttgatgt tcataggcat
    15121 ttagcggaag tgttttttcg tgagtccccc aaccaagctc tgctggagca atgccttctt
    15181 cataaaaacc ttcaactgac caggtgttaa caaattcgcc ccattgtttt ggcttattag
    15241 ttacttgggt gtcgcgctca gagatatgaa taactttaac tccaagtagg tgagcaagat
    15301 cgttataacg ctcatcagtt agagcacttt ccaccccagc tgcggccttc gattctttaa
    15361 gagcaagggt tgcaatatca accatggatt tcttcactag atgcgaaaca agtccaggat
    15421 ttgcgccatg ttcaacaatg gcagttgctc caactttgtt ccattttgat ttcatctcac
    15481 gcatacgcat atggcggtaa taaagagttc gctctagcgg atgtttattt gctcctcccg
    15541 cataaggatc ccactcctca actgaggtgt ttaaatagag aactccattg tcatggcacc
    15601 aattaagaat ggtattggca tcaatgttcc aagctaaatc aaggagaaag tctccagtac
    15661 ttaaatactt ttttaattgg ctatcaagat tatctttagt tatttggtct tggatgtaag
    15721 tagcgccttt atctagtgcg cctttaactc ttgagcgatt atctcgctgg tccatgatgg
    15781 tgatttgctt tgcatcaact agatgctcga ttaaaagggg taatactgat tgggatactg
    15841 atcctgctcc caggattaga actttatttt tgaacttctc tgccaattag ggaacctcct
    15901 tagcttgggg cttgtgaatc tcacctcttg tagttaagcc tttagggctt attggttgga
    15961 tgtttctgac ttggcaaaat ttacaaccta gagccagatg tgcctaattg gttatgccag
    16021 gaaaccacgc caagttttga tcgctttgct tcatacatcg cctcatcggc tcttcgaagg
    16081 agctgctcga gagcacttga gcctggatca tttcttgcct caccaattga aacatcgagg
    16141 tggatttcat ttgagttaat gtggaatgga taggtaagag ttgccttaag agccctagca
    16201 acctcaatgc catcacctcc tggaattaga gcgccaaact catcaccgcc gaggcgagct
    16261 agcaggccat cggagggcat aactctttga aagcggttag caacttgctt gaggagttga
    16321 tcgccaattc catgtcctag gctgtcattg actgtcttga atccatctaa atcaagaatt
    16381 agtagtgagc caggctcttt aaggaattct tggccatcca ccatgaactt tcgccgatta
    16441 gcaaggccag ttagctcatc ggttcgtgcc agtatctgtt cattactcat gttgcgagca
    16501 tcatttatag caacgcccat acgtagaaat gagagcgcga tagttacaaa agcgggcagg
    16561 attaaaaagc gtggaatgta tgaaggctgc agaactgcaa ttgcaagaag ggtagagctt
    16621 ccaagaagag cgattgtggc aactgctgaa ttgaaacttc tagctgctaa ctcttcatta
    16681 tttgcaaacc aaaaagcttg ggagagaagg ataaagctaa tcaaccaccc aacgtcagtg
    16741 agggatccaa attcatattt gcctaaatag gattgataga gaaagtagaa gtcagataga
    16801 aaaaatattg ttgccccgat aagaatcagg ccatttctcc agctcattga ctgccttaaa
    16861 accaagagaa gtactaaaac caagatgaca atgtctccta ccggataaag aattgccagg
    16921 aatacatcaa agatacttcc atcaatagtt gtcatagctg gtctgaccaa aagtgctgtc
    16981 aatatcgtgg tatatccaat tgcgataatt gaagtatcta gtaactcaag agatttgcta
    17041 agagtcttga atcggattgc tctagttaga ccaaaaatcg ctagtgggta aaaaagtgcg
    17101 tagaagcttt gagctatcaa atcaagatct agtagcgtat ttatctcaaa gaaagaatca
    17161 agtgatgagg ctattgaccc tgcgctccaa gagatgattg cgccaataac tccagctcta
    17221 cccaagggat catcgagaat tggagagaga gcaattgaga gtgcagcaag tagggcaacg
    17281 agattaaata gaaccaggtc atattcccac ctagcatctg aattgttcct gagaaatagg
    17341 taggcaatta gagttgcgat ggcaaggagg cggaaaagca gatagatttt catcatctaa
    17401 cggtattgaa ggggagagaa tggatagcga aaaaaatgta tcaagacggg cagtactttg
    17461 tggccttgct gttcttacgc ttggtttaat acctgaaaat gctattgctg caagtggtgt
    17521 aaaggtgcta gcaaatggaa aagtcgaggt aactctctcc aagaatccag ccttgaaaaa
    17581 agttggcgga gttgttcaat tcaccaataa tcaaggcata gatattgcgc tagtaagaac
    17641 aggtaaagga agtagtgctt ttaaggcttt aaaccttgct tgtacccatc aaggctatcc
    17701 cttgagaaaa gatggaaata agtgggtatg tgacaatcac ctagcaaatt tttcaatcac
    17761 tggcgctgtc acaaagggcc ctgcagaaag cccacttcag aatgttgcgc ttaaagtaac
    17821 tacaactaaa gtgatagtcg gctaactaaa actttatagg ccaggcttgc aggcttgctt
    17881 tctattttga agagttgaac taacctcaga cttagtgttc tcaaccgtct gaagcacatt
    17941 taccttctgc ttctcctttt tcgcaacttc gcctatcttg tcgcgaaggc tggcagccga
    18001 tctctcaaag ttcttggcag caagttcata gctacgagca gccttagtcc aagtggcagc
    18061 attagttgtg tattgcttac cagcagtggt ggtagctcca actttagttg cattatcggt
    18121 attggccttt gcctctaaca gcgcagctgc ttgtttagct ttagcatctg tagctttttg
    18181 atcaaatact ttagcttctg cttcatctgc cggagctgca gccttcaagg cagcaatctc
    18241 agtatcaagc atcgttgctg cttgggcagc ggtctctgtg atgctcttta agcgagcact
    18301 tgattctaga taaagtttgt tggaatcaat gcaccctacc cacacccaag ttaactttgc
    18361 attcttcaca attggattct tggtacatac atacccatct ccacctattt tggtctttgc
    18421 atttgccttt gtgcaagctg ctccagcttt gttagcagcc tctgccggaa ttaccgcagt
    18481 agttgccatt agcagggagg ttgcagcaaa tagggttgcg cctcttccaa attgcttgag
    18541 tgagttacgc acaattattc tccttaaaag ttgagtgacc tatatggcgc catcttaaag
    18601 ggggaactct gtttttttgg tgagggaaat gcgtaaagtt ctagagaatt tactgagaag
    18661 ttaggaaaat tacttgtttc tccctcggcg agagccaagg ttcattccga gttttctaac
    18721 ccaagatcta ggagcgtatc tagccagctt tgaaattact tgatactgcc ttccaggaat
    18781 gcagatagct tttccagctt gggcagagtc ccaggcgtct tgagcaactt tttcagaggt
    18841 gagccagagc caattgggca atccattcat tttcatgttc cctctttggt gaaattctgt
    18901 tctggtgtaa ccaggtgcaa gggctagtac ttttacattg ctactggaga gttcagtgtg
    18961 aagggattca cttagaactg ttaggtagga cttagctgcg ctataggttc cactagcaat
    19021 ccagcccgca atacttgaaa cattaattat tactccgcta tttcgagcct tcatagtggg
    19081 caatacagca tgcattaaac gcattggcgc agttaccaaa acatcaagca gggcaatctc
    19141 atctgaaatt tcacttacta aaaagctttt ctttattcca aagccagcgt tattaattaa
    19201 aacttcaata ggccttgagg tattctgtag acgtagctca acccgcctaa tatcttcatc
    19261 gcgactcaag tcagcaacta agacttcaac ttcaactcca tgattctctc gccactgact
    19321 agcgttttga tttaatcgct ctaaatcacg agcgacaatt actagatcgt gacctttttt
    19381 tgctagtagc tctgcaaagg atgcgccaat gcctgctgtg gctccagtta ttaaagctat
    19441 cggttttgac attactttac gatattactg gcatcttctt ctctgatatg ccaagaggtg
    19501 ccatattcct ctagtaaatc aaggaaaggc ttggcatcaa accattccgg gccatttacc
    19561 cctcttggct tccaaattcc cttttcaatt aactccattg caattattgg attaacagct
    19621 gtttgccaga ctaccgcttg atcgccatat tccttcatag accactgatt atcaattacg
    19681 ttatagatgt agacagcttt tggatttcct gacttgtcca aaccttttac taaagttcca
    19741 gcacaagtct ttcctttcat tcttgatcca agagttgctg gatctggaag tgatgctgca
    19801 agaagatctc ttggtgaaac tgatactccc tgaacatcta cattttcttt cttatccatt
    19861 cccagcatat ttatggtctt taggatctgg atgaactcag ctcccagtcc atatttaaag
    19921 ttaactttct tggcatcaat cttttgagga atcagaacta cctcttcgtg ttccacgttc
    19981 acacactcaa ctggtccaat accttctggg aaatcaaata cctctatctc gctaaatggc
    20041 tcagttgtaa accaaccacg accatcttcc caaacaagcg gaggatttaa acactcctca
    20101 atagtggtcc agatcgagaa ggagggagca aaatctgcgc cttcaactcg taagtttgag
    20161 ccatcgagaa ctgttattga atcaattcgg gagaagagtt catcattggc atatttagca
    20221 aagatatcgc tcattccagg ttctacgccc ataccaacaa gggcataaat ctttcgttct
    20281 tcccaattcc aatctcttgc aaactgctca tctcccaact tcacacctgt ttcacggtgt
    20341 gggtagtgag gatgcggcct tgataaagac attgccatat cgatgtaatt agtgttttcg
    20401 atttcgcaag caagaaaaat tggcattaca aatcgtggat caacagcatt aatgaccaca
    20461 tcaggatcga ctttacgaat taattcgcgc aggtcttcta gttcagcagc atttacctga
    20521 gctgcagaaa agcgtgggtc tttaacgcga tatacggctt cttcggcgcg cgagagggtg
    20581 cggtctgcaa ttaccatcga ggtaataaag ggccttcgca cagcgatatt tgctatcgct
    20641 cttccgacgc caccggcacc tattactagg gctttcatga ttagaaatca actccaaacg
    20701 agattgagcg ttaggctatc tgaaagtatt gaattaagca atttaccgcg taaaaatatt
    20761 gaattatcat tagatacttc acatttaata cgaaagtaat tatctgtgaa tagagtgtga
    20821 aattatttac acataagtcc ttgtagctca gtggatagag caaccgcctc ctaagcggta
    20881 ggtcgccggt tcaactccgg ccaaggacac aaacaaattc gttaattatt ttgttaagaa
    20941 atctcaataa taattagatg ttttttagta attcaatata tttcccaaga tttagaaaat
    21001 aaataaattg cgagaaaaat attcgaatta aatagactac tgaatttcaa ttaattggac
    21061 tcttttcaaa aaaagtacca ctggcatgat taccagtggt acttttgtag aaagggaaaa
    21121 tatgacgctc tgtgaactaa agcctcaatt gccgccactt cgttgagact tatcagtttc
    21181 aactttcttg ccaatgcggt ttgccacctc attaactcga gtgattagtt tagccttcaa
    21241 ctcagttgcc tgaattgttg taatctttcc actagctact gcagcatcga ttttctttga
    21301 gtcaaatgcc agcaaggcag caagcagtgc atcaaccttg ctgccagcaa tggttgcaag
    21361 ggattcaccc gcctggcgct tggttttgag tgactcagaa tcgatgccga ttgcatccgt
    21421 gattgccttg accttctctg ccttgtctac aggatttctc cctgcagatt tatcttgggc
    21481 tatggcgccg gtggcactca taagtgagaa tgcaagagct gcaactgcag atctcgccag
    21541 taatcctttt ctcattcata ctccttcatg acatatgttc ttacatcaaa ggcccttgcc
    21601 tcggatagag acaataaaag tccatatctt tgtgaaaatt ggggcttaaa ctttagagag
    21661 tttttagaat ttgccatact ttgtaaagtc taactgggaa aattctgaga ttagttacac
    21721 ctatttagta atgatcaaat tttgatggct tggttaagct attaccctat agccccctct
    21781 gagatctgac ctctctaaaa agtcgagcct tcataggttc gaattaatat atttaaatat
    21841 ttatggcagt aaatattgct gttgacttaa ttcttgagaa attaacgact caaatcttgc
    21901 ctggaattct tcaggatttt tgaaatctct cctccagcgt ccaaagtgcg ctctttcggc
    21961 cgttacttca gtttcaaagt aacgccgcat ttctaattca tctgagagtc ctacatgaga
    22021 aattaacctt cgatagcaat tctcacgatt ttcccttaca agattttcta ggaaaagtgt
    22081 caaatgttga ttctctgcaa ccaatttttt tgcttcagta gcaaggaaaa ctcggtcgcg
    22141 ccaccagata atggctctat cgggattatt tggaccccat ggctccttta acacagagga
    22201 gatattgtct agagcatttc gtcgcatttc aataaacttt gcttccggaa aaattctatg
    22261 gatgaagtct gcatacataa tgttcgctgg agttgtatcc atccagtaat tttcgccgtt
    22321 ccatttgtaa tgatttctca catatccgaa gacaaatttt cgagccgcct ccattggagt
    22381 gtttagagtc gagccaagct catctaataa ttcttccatt tggctaatac cgagagcccg
    22441 atgtagacca cttggcttac ctaatctatt tgtacgtatc caccaatctt catagatacg
    22501 tttgcgaaac tttacaaatc tcaattgaat cggatcatgt tgactaagtt tcgagaggag
    22561 ataacctttt ctagaaatct gtgttggaag aaattttctt agtccaaaaa caagatcgat
    22621 cagtccatga gtttcagtta taaatttaac ttcaaatgga gacccagcaa aaacgttaga
    22681 gtgctgcttg agaattctgc caacgattgt tgttccgctt ctgcccaccc ctcctgagaa
    22741 gacagggata atttccgtgg tcaaataatc tccaagttca atttagatcg gctgaagtcc
    22801 tacacgggac cttctaagca aagaattacc taataaattg tgaagagcat ttgattaata
    22861 tgtgagttga ttgctaagag tggattgaaa gcttttaggg ctaaggtgaa tatgcggtgt
    22921 ttatatatca gcccatgatt cacatgatat tccaaagatt ctaaacttag aattttacat
    22981 ttgccgatta ctctgacggc atgacttatg attccaaaac gctcctgcat tactggtcac
    23041 agaagccact atcacgtaga acgttactgg tcactgtagt tgtttcaact tttgcaaatt
    23101 ctgctattgg taaagctatt ggtgcaacgg caccaatagc aaacttcatc cttggacgcc
    23161 ctacttcaaa ttcaattgca ttgaatatct tagcaaccga aaaagttaga gcatatgtgg
    23221 agtatggtta tactaagtca aaatatacgg aaaaaacctc aattgatgct cttgagacag
    23281 gcgtacctaa agtcatagag cttaagaatt tgaaagctag ctccaaagtt tattatcgaa
    23341 ttcgttatgc agctggttcc gctaagactt ttcagacaag taagcaattc tcattctcaa
    23401 ctgcaaagaa ggtagcctcg ccatttacct ttaccgtgca tggcgataca catcccgagc
    23461 gcgctggaaa gatgttcaac tcagaccttt attacgtcac catggcaaat gttgcagagc
    23521 aacaacccga ttttcatatt cttatgggcg atgatttcag cattgatccg ttgatttcta
    23581 aaggacaagc aaatcaggcg aacgtagaaa agatctacag aactcatcga gactggctag
    23641 gtgtcattgg ttcgtcagtt ccgatcttcc ttgttaatgg aaaccatgag caggcagctg
    23701 cttacctact tgatggaacg actaccaatc cagcagtatt agccggtaac gccagattga
    23761 agtattaccc tcttccagct cctaatgatt tttattcagg gaactcaaaa gagattcctg
    23821 gagtaggttt gcctcgcgat tattacgctt ggacttgggg agatgcctta tttgtaaccc
    23881 ttgatcctta ctggcactcg aagaatgcag tcgacaatgt agcaggggtt ttagtagcag
    23941 atgatgcaag tggtgccact aaaggcggta aaaagaattc tgcaagcggt acgcaagggg
    24001 gaaataccgg aggtgccgga ggtgggggag gaaactccaa aaatttatgg ggaattggaa
    24061 ttggcgacga gcaatatgca tggtttaaaa agacacttga agaaagtaat gcaaaattta
    24121 agtttgtttt tgcacaccat gtcatgggaa ctggaagagg tgctgttgaa ctttcctacg
    24181 attacgaatg gggaggtagg gatcccaaag gtgtaacctc ctttgaaaag gagcgaccca
    24241 actgggagct gcccatacat gacttgatgg ttaaaaataa ggtttcaata ttcttccagg
    24301 gccatgatca catatttgtc actcaagaac gcgatggaat catttaccaa tcgatgccga
    24361 accctgcaga tgacaccttc tcctatttca atgacgcggc ttacaagagc ggaacgaaag
    24421 cgccaaattc gggccatgtc cgggttagcg tcactcccaa tcttgcaacc gtcgaatact
    24481 tcttagctgc aagatcgaag gactctggtc gaaagaattt gcaagttgct cacaaatata
    24541 cgattccttc aaagatttaa ctaggagcct agccttggga aagcttaaga attactctaa
    24601 tctttaatcc gcctgcctta cttggagaga gttctaatct tccaccccaa cgctttacga
    24661 ctttctgaat aattgacata ccaagaccgc ttcctccggc atcgcgagaa cgcgaactat
    24721 caaaacgccg aaatgccctt actccatcct cgtagaactt tttgggcaat cctgggcccg
    24781 agtccgcaat ctcaaaagtt actagttttc cagagtgacg acaattaatc tctagtgggg
    24841 aattgtccgg ggtatagcgc ttaatattag aaaaaatatt tcctagcaat ctatcgagat
    24901 cttcttgttc aataacggcc acgacattat tttcgattga ggtgtctatt ttgcgctgcg
    24961 gttggagttc ctttagttga gcaacttgat tgtttaaggc ggttgaaaca ttagaatgtg
    25021 aatcgaggct catttcttta gattctaatt cagctgcatc taaaagatca ttaattagtt
    25081 tttgcattct gccaatctcg cggtcgacat gcctctcata atcttgacgc ttgtcattat
    25141 cgcccctacc gcctttaatt aaatcaaaat atcctcgaat tactgtgaga ggtgttcgta
    25201 attcatgagc tgcatcgcct agaaattcca acattctttc ttgattttca tctagtgctc
    25261 gggcagcgcg attgagttgc gcatcccttc taaagaaaaa gtatgagaat aagatggcta
    25321 cgattaaaac taatattgtg aagattccta aataaagttg gtttttccct cgcagatctc
    25381 ttagctctga taaagagtag ccaacaacta ggtattcagc ccctgaaagc ttcaaggagc
    25441 gaacccgact ctctccaaga ttaatgggct tatcagatgc gcgttcaaga aaatttaagt
    25501 ctactgaatc tgaaatttcg ccagatttgt caatcactac taccacttgg aggtcttctg
    25561 taatgaggct caatgagaat ggaaaatcgg attgatctgc aatgaaagtg gctacagtta
    25621 gcggatcttc tgaagattgc tttaactgca gaattacatt ttcaatcttg atgtcattga
    25681 gacttaattg ttgttgatat gactgagaga ccgcaaaagt tccaattgag gccgatacca
    25741 gagtcacgat agatgagagt aggagtatta gcctgatttg aagattcatt actctcctag
    25801 gatttggaaa ccgacacctc ggactgtctt aattccttga taaccatctc gatgaagttt
    25861 acggcgcaga tatgagatgt aggtttcaac cacagtcgtg tttgatggga aatctattcc
    25921 ccaaacctcg gctagaagta actccttctt tactactttt ccagaacgat ttaataatcc
    25981 ttcaagtagt gagaattctg ttgctgaaag ttcaactttt tcactgttaa aggtgacgag
    26041 gtgtctttct tggtccattg ttattggacc acaaattaat tttttcgact ccgctgaagc
    26101 tggttttgtc cttctaagaa tagcttttac gcgaagaatc aactcttcaa ttccaaatgg
    26161 tttgattaag tagtcatccg cgccaatttt caatccttgt gttatctcat ctctttgtga
    26221 acgcgcgctg agtacaagta ctggaattcc tgaaccctct tcacgaattt ttgatagtag
    26281 ggcaaagcca tcgatcttag gcaggttcac atctagaatc atcaaatcaa aactttgacg
    26341 tctcaactta tttagtgctt ctaaaccatc tcctgcttca tcagtatcaa cttcaattaa
    26401 agagagtgaa tcacgaatta ggtcacgaat tgattgctcg tcctccacca ctaaaatccg
    26461 agtattactt gccatgaggg tagattaatg ccatagggtg aaagaaagag gctagaattt
    26521 tatttgatag cgggattcga aagaattcaa tgcctttttc acaagattct cacaaagatt
    26581 taagcaaaca ttctcacgtt cgaccaataa atggaaagga attaaattga gaagaattac
    26641 tcttattgcc cttatttctc ttggggtgat tttcggatcc agcacagcta ctttcgcaga
    26701 aggaaatggt ggtaataaga cagccatcac agcttggaag gctgagaatc aagccaagat
    26761 ggatgcttat caggctgcgc tcaagaagta tcttgaaatc aagaaagcta atgaagaggc
    26821 acgaaaagca atcgcaacta agtttaagtc tgatgcagat gccctaaggg catccacaaa
    26881 ggcagcagtt gaagctgcag caacggccga agccaagaaa gttattgcca gggctagcaa
    26941 agattcgtta gaaaaattaa ttacagaacg aaaagttgct cttgaagctt taccaaatcc
    27001 aggcgctaag ccagttaagc ctactttgac tccaaagcct gccaagccat ctccaaaggc
    27061 ttctccatca tctcgaggtt aagtaagaaa ataacagagg ggtcagggca ccacaaaagt
    27121 ggtgccctgt tcaattaatt cctttaaatt attacagcac gactactatc aaaataatcg
    27181 taaaaatctt aatcagttac tcaccggttc aactccggcc aatgacacac ttaattaaat
    27241 attactgttc tatttccaac gataaatatt ctatcttcag cataaagttt tacagctctt
    27301 gctaaaacac gacgttcaat atcgcggcca atagcaatta actcttcagg ggttgatgca
    27361 tgagaaacat gagctacatc ttgctcaata attggaccct catctaaatc tttggtgaca
    27421 aagtgagcag tagctccaat aatctttaca ccttttgcat gtgcttggtg ataaggctta
    27481 gcccctttaa atcctggaag aaaggagtga tggatattaa taattcgatt cgagagtttg
    27541 tcgcagaaat ctggggagag tatctgcatg tagcgagcta aaacgagaaa atcaattttc
    27601 tcagagtcaa taagtgagag aagttgagtc tcagagcttt ccttattttc aatagatatt
    27661 ggaagatatt taaattttat gccatgagat tcgactaggc tctttagctc ttcatgattt
    27721 cctacaacta aagggatttg aatcggcaat tcgccaagtt ctagtaggta gagcagatct
    27781 cgtaggcaat ggctctcttt agtaaccatt actaaggccc gcttgggtga ggcgttatct
    27841 ctgatagtta actcaggatt gaattttgag agcccttcaa ataagacctc tcgagcagta
    27901 tcaagtgagg agctagtttc aaagcgagtg cgcataacaa agctattagt cactggatct
    27961 gtgaactgct gattttcaat gatgttgcct tggcaggcta agacagagct tgtcatcgca
    28021 tgaacgatgc ctctttgatc agcgcattgc agggttgcga ttaaatccac ttgggaattc
    28081 tacctgcgca gaatccttta gtgattaatt tggaagcgct taagtgagcg tggagcccac
    28141 caattccagt caccaaagag tctcatcaga gctggcacta gaaatgcgcg aatcaaagta
    28201 gcatcaagaa gtattgcgaa tgcaacgcca aatcccatca tcttgattga tgtcacaccg
    28261 ctaataacaa aggcagcaaa gaccactgcc agaataaacg ctgctgcggt gatgattcgt
    28321 gctgattttt gcaggcccat cgcaacagaa tcaatattgg aatttccagc atcatgctct
    28381 tctttaattc gtgagagtag gaagacttca tagtccatag ataacccaaa ggctacaact
    28441 gcaattaaga ccatggtatt tgtatcgagt gaaccagttt caatgaaatc tccgacaaga
    28501 aactttagat ttccttcaat gaatacccaa gtaagaactc ccatggttgc agccagcgag
    28561 acgaaattta atatcactgc tttaatcgga agtagaattg agccagtaaa ggcaaagagc
    28621 aatagcagaa ctgtgattat gatccagcct agaacggcag gaagagtgct agagattgca
    28681 ttttgtgtat ctgcataatc tgctgcggcg ccgcctatca aggttccact aggtgcgggt
    28741 agattgcgaa gctccttaat tagagcctca ccttcactag ttcttggctg catcgaatgg
    28801 attgcaacca cgcgcgcact ttctccagca agctctactt ggccaacgcg agttatgcct
    28861 tcagttagtg aaactcggtt tacaaatgcg ttgatctcac tggtctgtga tgcgccattt
    28921 ggaaagacta tttcaatcgg attgctctct tggccgggaa attcatcggc aataaattga
    28981 gatgcgatat atgcccgatc atctctagga agtacaccac tatctacttg agagaatttg
    29041 atattggcta tgggagaaat catcaagaag agaacggcta aagatgcagt tattacgctg
    29101 atcggtcttc tcatcacaaa tcgcgcaacc tttgaccatc gaccatcttc tttttgaact
    29161 attccacctc tacgaactac gcctttatta atttttgttc ccaataaagc cagaattgca
    29221 ggtagaggga tgagcgctcc aacgacggca agggcaacta ctgcagctcc ggcataaccc
    29281 atcgatttta agaaattctg tgggaagaat gtaagtgata agagcgtaag aacaacggta
    29341 actccggagt aaaagacagt ctttccagca gttcgaaggg tatttactat tgcgctttca
    29401 ttatcaagac ccttagctaa ttcttctcgg aatctattaa ctaccaatag agcgtaatca
    29461 attcctaagc ccaacccaag tccggtagtt aaattcaaag caaaaatgct tacatcagta
    29521 actagagtaa gtaggaacat aactaggaat gttcctaaaa tcgctgtgat tccaacaatt
    29581 agaggcatag cagatgctgc cattgctcca aatacaaaca agagaagtaa gaatgtaagt
    29641 gggattgaga tagcctcgga gatctttaga tcattttgaa ttcggccatt gatagcattt
    29701 gcaaatactg cgccgccaga tgcataaact tcaacgcctt caaaagctcc atcgtatttt
    29761 tcttgataaa acccaccgag tttatctatc tcagtaaagt ctgttgattt tagataaacg
    29821 aatataaatg cagaatttcc atctttgctc tttagtgagg gagcgccacc tgccgaccaa
    29881 taagagagaa cgttttcagc acttggttcg cttctaaggc tggcctcaat tttctgtgcg
    29941 gcagcaacta cgcttggatc atcagccgac ttatcttttc catcaactac caatacaacc
    30001 gctgggtctt taaccttaaa agtctcatcc agatactccc aaacttttgc tgagtcacta
    30061 ttgggatcca tatatccacc ggtatcaaag cgagaaaaga cttgagaacc aatggcccct
    30121 gcaaggattg tggagagcag gaaaagggtt agaactgact ttttccgctt agctattaag
    30181 tgtcctaatt tttcaaacat ggtgtcataa taactgtatg agtgaagtgc ttcctgctat
    30241 aacttccgat gaagtagaaa ccgagataga tatcactcgc gatgaagaaa ttaaaggcga
    30301 gaagccccct catcactaac aactcaattg acttttagtc attctttgcg ggtggcggtg
    30361 tagcgctggt tgtaggggtt ggctttggcg cctcactctt tttaacttgg gaatcagtct
    30421 tataaaagcc agaacctttg aagacgattc caacatttga gtaaatcttt cgaacctctt
    30481 tgccacactc aggacaaatt gagattggct catctgtgaa ggattgcact atctcaaagg
    30541 catgttcaca ggcggtgcat tgatattgat atgtcggcac agcgtaatct tagggagtat
    30601 gaattacttc ggcgaatcgg aatagcaggc gagcatgttc tttgatctag caaatagcgc
    30661 tctagctgtt ttggtaatcg gactattaat atccctcgcc tttctactgc cggagaatca
    30721 gggtcgacta gagcagagcg cttcgacaac tcttcaaggc ttaagaattg tttcaggact
    30781 atggtttctc atttcgattg gttatttatt gaccagcctt gctgaaatat ttggcagtgg
    30841 tattggcgag attttaaaag tgaatatctt gcaatcattt ataactcaga taacacttgg
    30901 aaaattgttg gcttatcaag tcatagttgc gcttatagtc tttatctttt cgaatctcat
    30961 aaagaaaaat ggaggcgcgc tagcacttct tattttggcc cttagtggga tagttgcccc
    31021 actgtttcaa agtcattcca gctctcaagg cagccactca ttagcgattg gctcgcttgt
    31081 gattcatgta attgcattga gcttttggct tggcagtgtc attgccttaa aggtgatgcc
    31141 aagtgagtta caggactttg ctttctccag agtttctgta attgctctct ggtcatcact
    31201 atctgttgtc ttaacaggaa tagctaatgc ttggaccagg ctccgtttga gccaagattg
    31261 gtttactgga tatggcgcct taatatctct aaaggttgta cttacgcttc ttgttttctt
    31321 tattgctagt agggtgagaa agaatttatc ggtcaatact ttagtcgcat ttgaaatcgg
    31381 gataatggcg gcgatattgg gaattggatc aatactcaat cgtttcactc caattgaacg
    31441 tggagaaatt gaatttgatc gcatcagaga gctggtcgga atatctatgc caagtgaacc
    31501 caccctttca agagtgttct ttgaatatga agcaaatggt ttagcacttg gcgctcttat
    31561 ctttgttact gcgctatata ttcgaggcgt agttgcgctc gctcgccggg gagatcgctg
    31621 gccagttggc agaacaattt cttttgcgat tggaatttca cttctagatt atgcgacgag
    31681 tggcggatta ggtctttact ctcacttctc ttttcagtat cacatgattg cccatatggt
    31741 tttaagcatg attgctccaa ttgcaatcat attaagcgct ccaatcacac ttgcactaag
    31801 aactcttcct attggtcggg ataaatcgga gcgaggaatt cgaggcatgc tgatacaagc
    31861 tcttcactct cgcccaagta gagtcataac tcatccagtt tcagcgctag caatatttga
    31921 tggctcactc tttgcacttt atttcactcc acttttttca aatctgatga gtggacactt
    31981 tggtcatctg attatgaact ttcatttcgt tgctgccggc ttgctcttct ttcatgtcat
    32041 agttggaatt gatccaaatc ccagaaaggt tcatcattta gttcgagtag taatacttct
    32101 tgcagcgatg agcattcacg cctttttctc ggttgctttg atgtcagcta atgaattaat
    32161 tgatggtggt ttttatcagc tcctagatcg accttgggcc acagatcttc taagtgacca
    32221 aaagatgggc gcagctattg ggtgggcgat gggagagata cctattgtta ttgcccttgt
    32281 tgcaactttt attcagtggg taagaagtga tgccagagaa gccaagaggg cagaccgtcg
    32341 ctctaacaca gaacttgccg aatataacgc ctatcttgag caactttctc gcaagaacaa
    32401 ttcatcacaa gataagtagt attacgccat ggaaaatctc ttcgaccttc ctgaggaata
    32461 ccaagcctta agagccagtg ttcgctcact tgctgataaa aaaatccaac ccttcgctca
    32521 tgatgttgat gccaattcca gatatccgca agaagcattt gaagctcttc aaggcgcggg
    32581 tcttgcagca gcccatgtac caactgaata tggcggcgat ggagcggatg ctcttggcgt
    32641 agtaattatt atcgaagaag ttgccagagt ctgtgcttct gcctctctaa ttcctgctgt
    32701 taataaactt ggatcggttc cccttatgtt aggtggaagc cctgaacaac aaaagcgctg
    32761 gctgacaccg cttgctcaag gtaaaggttt ttcatattgc ctctcagagt ctgaggcagg
    32821 atcagatgct gcttcaatga aaacaagagc ggtcagaaaa ggcgatggtt gggttttaaa
    32881 tggaagtaag aagtggattt caaatgccgg agtctctgag ttctatacag ttttagcatc
    32941 aacagatcca gaaaaaggcg ccaagggaat tacagcattt atcgtagaaa aaagtgatcc
    33001 tggtctttca tttggagcgc cagagaagaa aatgggcttt aaaggatctc caacgcgtga
    33061 ggtctactta gataatgtag aaattagcga tgatcgcaga attagtgaaa tcggtgcagg
    33121 tttttctctt gcgatgaaga cccttgatca caccagaatt actatcgctg cccaagcgct
    33181 cggcatcgct caaggtgctt ttgaagttgc aactaaatat gcacatgaga gaaagcagtt
    33241 cggcaaacca atctttgatt ttcaagcagt gcaatttatg ttagctgaca tggctatgga
    33301 aattgctgct gcaaggcagc tgacatatgc cgctgcaagt aagagtgaaa atagtgcaag
    33361 tgatttaacc ttcttttctg ctgcatcaaa atgctacgca actgatgtag ctatgaaggt
    33421 aacaaccgat gcagttcaag ttcttggtgg ttatggatat gtcagtgatt atccagtgga
    33481 gcgaatgatg cgagatgcga agttaactca aatttatgag ggaaccaatc aagtccagcg
    33541 cttggtaatg gctcgcaacc ttaattcttt gctgtaaacc taaccaagat ctcaggggtt
    33601 gctgcagcat ctactttgca atcaaagctc tctacaggaa gactctcact agttagttcg
    33661 ccgctataga ccagggcaac cttccaggca tttagcttcg ctagcgcgcg atcatatgag
    33721 cctccacctt gtcctaggcg atttccttgg cgatcaacgt gaagagctgg aactatcaca
    33781 acatcaatct tgcccttaaa gctctcaccc tttggtgact ttaaattgcc atcaatttct
    33841 agatcttcat ctttgccact ccagataatc caatccaaat cattgtctgc aaggcgtctt
    33901 ggcaataaaa gagtcttacc actttgaatg agggaattgt ttagctctga tgtatctggc
    33961 tcacttccat aggaatagta acttgcaaga acctctgcac ttttaatttc tttactatcc
    34021 aataaatgtt gccaagaaca ctctgaaagg tgaaactctc tctcacttcg aaagcgtttt
    34081 cgaagttctg acttaattaa tgacgcactc tggcttgaat ccacgggatt agcgtagata
    34141 caaagtaggg ttactacgtg agcgaaacga ttaaggtggc tgaacttgct accgaactgc
    34201 tagcgctagc aaagccgctg gctgcctttg atatgccatt acttgatgcc catggcgcaa
    34261 ctttggccct tgatgtgagc tctggtgagc aggttgcact caaatctggc tcaagaatcc
    34321 gcgcaactca aataggacta gctgcatctc ttggtttaga tcggcttcca actagacctc
    34381 agccaagagt ggtgattgtt tcggcaggag atgatttagt cgaaccaggt tcaccgctta
    34441 gagacggcaa agatgaatat gagactaatt catggctatt aacaacagca gtaaaagagg
    34501 ctggagctgt gggatatcga gttcatacca ttcctgaaaa tgctgctcaa ttaaaggatg
    34561 taatcgaaga tcaattggta agggcagatt tactggtcat ctgcggtgag agaaatgatg
    34621 agtcattttc tctaatctat tcagtcctta atgagcttgg aaaagttcgt gaggtccttc
    34681 ccttaattga gggaagtggt aaacatgcat ttggtgtggt tggtccggat cagactcctg
    34741 ttgtttcact tccaggagat ccgatatttg catatatttc agcagagctt ttcattcgcc
    34801 caatgatacg aaacatgatg ggactttatg acatccatcg accagtgatt aaagcaacct
    34861 taacaagtga tgtattaagt gaggttggaa agcactcctt tataagaggg attctctcaa
    34921 gcgataaacc agagcgaaga ctcattactc caattgaaaa tcaaggagat ttaattggat
    34981 tatctgatgc cacaggtcta atagttatta cagagggtga aagtggcgca agtgctggcg
    35041 ctgaagttga tgttctagtt ctcgagcgac gttttaattg attttatggc aagtatttat
    35101 agtcatagct ggcccacagt tctcaaagaa gatcagataa cccttagacc acttagattg
    35161 cgcgatcgtt cagcttggtt agcagttaga aaagttaata gagcctggct ctctcagtgg
    35221 gaggcaacct caccccttaa ccatgagaat aagaatcttc ctagctactt tgagatggtt
    35281 aattaccatc gaggcgaggg cagagctgga agatcgttaa ctctggctat ttggtttgaa
    35341 aagaacttaa ttggtcagat cactttaggt ggaatctcct atggcgcata tcgaggggga
    35401 catatcggct attggattga tgagcgatat gccaataaag ggataaccac cagggctgtt
    35461 aggagattga ctgcttatgg ctttgaggaa cttggtttac atcgcatcga aattaatcta
    35521 agacctgaga atgcggcatc aaagagagta gctgagaagg caggttatgt atttgaagga
    35581 cttcgtcctc gctatcttca tattgatggt tcatggcgcg accacctctg ttatgtgaaa
    35641 gaaaatccta gaatttagag ttgttagaat ttcaaaaatc ttaaattttg tatcaggcaa
    35701 ataccagaaa agtccaaccc ctacctttaa ggttatcttt catgggatca gggctcatct
    35761 acataatcat cattggcatg tggattgcct actttctgcc ccgttggatt tcaagccatg
    35821 aagagataag cggaagatct gtcaaacgat ttgatgaagc tatgaaagtt gttggaataa
    35881 cttctggcca cactgctcca gatattgaat ctctaaataa gaaacgcgaa catcagattg
    35941 caattcgtag aattcaattc ttctccatcg ttggtctaac aatcgttgta tctctattta
    36001 ctttagtagg tctactttct ccagtaattc ttcttctacc aggatcagtc tttctaatgt
    36061 atgtagtcaa tactcgatat caaattagtt tgataaaaga agaggtaaag cgagttaatt
    36121 ctgcaaatag aattaccagt aaatcttctg cagcaaatta cgcagagatc attggaaggt
    36181 caaagcgcgc agcatctctt gcaagtatta ccgaaggttc aggtttgagt gaagagcagt
    36241 ggcttccact ttcagagcga ctctcttctt accaagatag tggaactatt actttgctcc
    36301 ctaaaggaag tgcaaacaaa gatgataaga gttgggatcc agtctcagtt cctgcgccaa
    36361 attatgctaa ttcatcaaag gcagttcctc gtcgcgtaat tgacttaact attcctggtg
    36421 cttggagcga ggcgcaagaa aagattatgc gcgaagtatt ggcgccgagt gcagataaga
    36481 tctttgatca agagattgca gatcaatacg aagaacacct tcgcagcaat agagccgctg
    36541 gtcagtagtt aaatccagct aggaaaccac attcgcgcat accaatcttg gtatggcaaa
    36601 actgatccaa catagagtgg caaaaagtag gcaaaagtaa ggccaattaa gccaatagtc
    36661 actagggcat acctcttcct aaccccccgt agctcctgat tttccagggc ttttgccatc
    36721 acataaatca gagccaagat caaataaggc tcaaaggcga tggagtagaa gtagaaagta
    36781 gttctctctg gaaatgcaat ccacggaagg taatttgcaa atagaaagag cagaattagg
    36841 ccagcgttta attctcgttg atatatgaaa taacctaatg taatgaaaat agatacaaga
    36901 cctaaccacc aaagtgtggg cgttcctaga gccagcactt cttgtgagca attttcttga
    36961 ccacattgtt ttggagtggc atagaagaaa gatgttggcc tgccaagaat tagccaattc
    37021 caaggacttg cttcatatga gtgatcagtt tttagatttg tatgaaaatt tagcatctcg
    37081 cgatgataat taaaaagcga gaatagagaa ttacttgctg aatctctctt ccaaccaata
    37141 tcacttaaaa accatccact ccaagtaatt aggtatgtta aagcgggcag aattgtaaat
    37201 tgcagggcag tctttatata tgtcttcttg taaaagagta agaataagca gagcgctact
    37261 aagagataga gcgcactcca cttagttgct agtgctaagc cgatagttat gccagcttgc
    37321 caatactttt ctttaatgac tagataaaaa gtgagcaaga taaagaatga caaaaatata
    37381 tctagtagcg cggttcttga cattacaaga tggagtccat caagtgagat cagggctgtg
    37441 gcagataaac ttaaaaaatt agatttaaac aaagcttggg tgatgaaata gatgagagcg
    37501 actgaagtaa ggccaaataa agcggcgcta aatcgccatc caaaagggtt atctccgaaa
    37561 gtttgaattc ctatcgcaat tagccactta ccaagtggtg gatgtactac aaactctgct
    37621 tcacctgatt ggctttctac cccgtaggca agaaagtctc ttgcgccatc tacataataa
    37681 acctcatcaa ataccaattc attaggccta gctaaattgg caaaacgcgt aattgctgca
    37741 aagaataaaa tgattgccag tgctatctga ttgcgatagt tgagaatcgg cctagccatt
    37801 aaggcactca tgatagataa ggatactaag ggccataatg aggcagtgag cgagttgatt
    37861 ctggcaggag tgccccttgg aaacattggt gatgcctcaa cgagattgaa ggcagccatt
    37921 tcaagcgcgg atgtagttct tgccgaagat tcacgaaggt ttaaccgcct atgcaaagac
    37981 cttggaatcc aatgtaatgc cgaagttctc tcattctttg aaggaaatga aagctcacga
    38041 cttgatgaag tggcgagtca tttagcatca gggcgccaag ttttattggt aagtgatgca
    38101 gggatgccat cagttagtga tcctggatat agagcagtga gaatggcaat cgataaggga
    38161 tttaaagttt cagtaattcc aggtcctagc gctccgcttg ctgcacttgc tatctctggg
    38221 cttgcaagtg attcatttgc atttgatgga tttgtgccaa gaagctctgg cgcacgtgat
    38281 caattctttg aagaccgcgc tatggagact cgaactctga ttgtctttga agcgccacat
    38341 cgaattagtg aatcactcca atcaatgatt aagagctttg gtccaaatag acaggcagca
    38401 atctgccgcg aattaacaaa gacctatgaa gaggttgtgc gcggcgaact agcagaatta
    38461 cttagctggt ctgaaagtaa agagatgctt ggagaattca ccatagttat tgccccctat
    38521 gacccaagcg agcaagttgt aagcgatcaa gaagtagttg aaactgtctc gcgctatgaa
    38581 gcatctggaa ttactcgcaa ggaggcaatt gccttgactg ccaaagagct tcacatacgt
    38641 aagcgaaaag tctttgacat catggtggaa cagaagtaga tagaattcag cccatgtcag
    38701 ataccaataa gtctttctat ctgactacac ctatttatta tgttaatgac gcccctcata
    38761 tcggacacgc ctacaccact gtggctggtg atttattaac tcgatggcat agacaaaggg
    38821 gtgaatctgt ctggtttcta actggaactg atgagcatgg ccagaaagta atgcgcacag
    38881 ccgaaactaa taacacagcg ccgcaacaat ggtgtgatcg attagttgaa caggaatgga
    38941 agccggtttg gaagcattta gagattgcaa acgatgactt cattcgcact actgaagtaa
    39001 gacatacgca gcgagttcag aaatttctcc aaggattaat ggagcagggt tatatctatg
    39061 aaggcaaata tgaaggtccc tattgcgttg gctgtgaaga atttaagtta cctggagatc
    39121 tgattgaagg aaattgcaaa atccattcaa agccagttga aatgcttagt gagacgaact
    39181 ggtttttcaa gttatcagag tttgccgccc cacttcttga gcactacaag aaaaaccctg
    39241 aaagttgcca acctgagagc gctagaaatg aagtaatttc atttctagaa ggaggagttt
    39301 cagatctatc ggtatctcgc tcaacttttg attggggagt aagagttcct tgggatgaaa
    39361 agcaagttgt ttatgtttgg tttgatgctc tcctaaatta tgcaactgcc gttggcctaa
    39421 ctgatgaccc atcaagtgat ggtggtaaat tctttgaaaa gacttggcct gctgatgttc
    39481 acctagttgg aaaagacatc ctgcgttttc atgctgtgat ctggcccgca atgctcatgg
    39541 ctgcaaaact tgctctgcca aagaaagtat ttgcccatgg ctggctctta gttggcggcg
    39601 aaaagatgag caagagtaaa ttaactggaa ttgctccctc agatatcaca gatcactttg
    39661 gtgttgatgc cttccgttat tactttatga gagccatacc ttttggaagt gatggatcat
    39721 tttcttggga ggatatggct gcccgctaca caagtgagct tgccaatgac tttggaaatc
    39781 tagcttcacg ccttgcagca atggttgaga agtattgcga tggcattctg ccaaatccag
    39841 caaaagatga agagctagca agcgctctat caaagactgt cgagagcgca gataaagcaa
    39901 ttgtggctct agattttcaa ggtggaataa atgccatcat ggatttctgt aaggcggtta
    39961 atggctatgt cacgatacaa cagccatggg ttgttgctaa agatgagagt aaaaaaagtg
    40021 aacttgatgc aattctttat aacacggcag aatctttaag ggcccttgca attcttctcc
    40081 atccagtgat gccactttca actagcaagc tatggtcata tttaggagcc gaaggctcac
    40141 ttggaccaat tggttcgcag aaaatttcag atgttgccaa atggggacaa ttaaagggcg
    40201 gaagcaaaat aattaaagga gatattctct ttccccgtct tccggatata gagagttaaa
    40261 aaacttagca aatggctgat cgaaataatc gcgaaagtga tcgcagagct gccccacttc
    40321 cagagccact gcccacaact tgtgtggatt ctcacgccca ccttgaaata gttaccggcg
    40381 cagcagccga tcatgatgat gttaagaaag tcttagatga tgcaagaagt gttggtattg
    40441 atcgagtagt gcaggtcggt atctctgtta aggaatctca atggtcagtg gattgcgcca
    40501 attattttaa tactcaagtt ctagcagctg tagcgcttca tcccaacgat gctcctttaa
    40561 ttgaagatct tgattcagat ttagaggaga ttaatcggtt agctggtgaa aagagagtct
    40621 gcgcaattgg tgaaactggg atggacttct ttcgcactga agaaagcctt agagataaac
    40681 agaaatactc attcaaaaag catattgaaa ttgctaaagc tcataagaaa gctctcatga
    40741 tccatgaccg tgattctcac cgagcagtat tagatcttct tgatgaagta ggggcgccaa
    40801 ctcataccgt ttttcactca ttctctggag atgctgatat ggccaaagag tgcgtcgcta
    40861 agggatatct cctctctttt tcaggaacgc tgacctttaa aaatgcccca cttctacacg
    40921 aagcgctaat gcatgttcca ctagaaaact tgctggtaga aacggatgct ccctttctaa
    40981 ctccaactcc aaatagaggg gctcataaca ctcctgctca aattccacac attttgcgct
    41041 ttatgtcaga gcgtagggga gaggatctaa caactcttac cagagcaatt agtgataatg
    41101 cagagcgcgt atttggatcc ttcatcagat gagcatcacc ttacttggcg caggtgagat
    41161 taggcagatt gcagagtcct tagaccttcg ccccacaaaa tcacttggtc aaaactttgt
    41221 aattgatagc aatacctgtc agaaaattgt tcggttagcg ggaattacat caagtgatca
    41281 tgtagttgaa atcggcccag gccttggatc tctaacccta gctctgctac aagccagtaa
    41341 taaagtaaca gcaattgaaa tagataaacg tctctccgat caattagaac tcaccgctaa
    41401 gtctcatggg gctagttcga aaaatctgat aatcgttaat gctgatgcta tggaagtaaa
    41461 tgaaattgcg ggaaggccag ataagttagt ggctaatctt ccctacaaca tctccgtgcc
    41521 tgtcttatta aactttctag aaaagtttga atctataact tcaggaattg ttatggttca
    41581 aagtgaggta gccgagcgac tagcagcaaa gccaggcagc aaaatctatg gagtccccag
    41641 cgctaaggct gcatggtggg ctgatctaaa actagctgat tcggtttcac gttcaatttt
    41701 ttggccagtt cctaaagttg actcctcatt agttaaattc acaagacaca aacctttagg
    41761 agatgaaagg ctacgaaagc tcacttttga actagttgat cgtgcatttg ggcagagacg
    41821 aaagatgctg cgctcaattt atgcagatct tgccggggga tcagcccagg ccgagcagct
    41881 gctaatcgat tcaggaattg agccaactat tcgcggagaa gcgttaacaa ttgatgactt
    41941 tctggcaatt gctcgtcaat tagataggcg ttaatagcgc tgtagtaata ccctcaccac
    42001 atgagcgccc aagttctggt tcgagttcca ggaaagataa atcttcagct ctcagttggg
    42061 ccgttgcaaa aagatggatt tcatccagta gcaacagtat ttcaagcagt ctcaatcttt
    42121 gatgatgtaa aagttgaact aaatgacgat gggctagtac gcgttcaatc gacaggaaaa
    42181 actaataacc atctgccgct agataaagag aatttagctg ttaaagctgt cgagctaatg
    42241 cgtaagaaat ttagtatttc agatggaata actattacct taacaaagga aattccaata
    42301 gctggtggta tggctggcgg aagtgctaat gcaggggcag caatagttgg aatggatagt
    42361 ctttttaatc ttggtctgaa acgaaatgat atggaaaaaa ttggagcaga acttggagca
    42421 gatgttccat ttactatctc cggtggcact gctattggaa caggcaaagg agatcagata
    42481 actccagtac tttctcgagg aacatatagt tgggttcttg ctctctctag ctctggatta
    42541 tcaacccctg cggtttatag tgaatgcgat cgcctacgtg aaggtctgca agttgctcca
    42601 ccacaaatta gtgatgcgct cttgcaagca cttagttctg gagatagcaa taaacttggt
    42661 aaagcgttaa gtaatgattt gcaagcagca gcatgttcac taaaaccagc gcttcgtcta
    42721 attcttgatg ttggtctgga ttatggcgcc cttggtggaa ttgtctctgg ctcaggtcct
    42781 agcgtggcat ttttggcaga gcatgaagat catgcgctag atcttgttgt tgcactgacc
    42841 tcaagcggag tagtaggtaa tgtggttaga gcatctgggc cagtacctgg agctcgggtg
    42901 atagagagcc tataaatatt gagcgttatg gagcatccag cctgaggcaa tagaattcag
    42961 gttcctccat tgtgtagtgg ctagcacatg ggcctttgaa gcccagggtc caggatcgat
    43021 acctggtgga ggagcaagaa ttaagcggga aagagatagc tcaaaaatta aatagaatag
    43081 ggccaggagc gcagagaggt ggggagttga accagctgga tctcgcaata ctcctcgctg
    43141 ctggcgaggg caaacgaatg aaatcctctc gcgccaaagt tctccacgaa attgccggtc
    43201 gatctctaat cgcacatgtc atcaaagcaa tcgaatcatt agcagcaaaa gagttaagag
    43261 ttgtagttgg agcagataaa gaagatgtaa tccaacatct aaatcaaatt tcaccaaatg
    43321 ctaaaacaat ctatcaagaa gttcgtggtg gaaccggcca tgctgtaaaa attgcgctcg
    43381 ctggacacaa tccatcggga acagttttag tctgcgctgg tgatactcca ttactaaccg
    43441 gagcaactct taaagacttg ctcaaacacc atgtctcaac tggagcaagc gcaacggtat
    43501 tgagtgccaa catgccagat ccaagtggat atggtcgagt attgcgagaa gcaagcggag
    43561 atctggcaga aataattgaa gaaagagatg ccacagagat tcaaaagcag acctctgaga
    43621 ttaactcagg ggtctatatt tttgccgcat cagatttagt agcagcgcta ggcaaaatag
    43681 gtaaaaataa tgcgcagcaa gaagagtatt taactgatgt gattggcatc ttgaagtctg
    43741 agggcaagaa agtcgtagcc tttgaaactc cagatttcac tgagattctg gggataaatg
    43801 atcgctccca gctctcgcag gccggtttta taatgaatgc tcgaatttgt gatgcattaa
    43861 tgcaaagtgg agtaacaatt acagatccga tgagcacatg gattgatata acaagtgaaa
    43921 ttgatattga tgtgacgatt gagccaggta gtgcgattaa gggcagtaca aaaattggaa
    43981 gagcagctgt aattggaccg cgaactactc tgattaattg cgaggtagga acgcaggcgc
    44041 gagtctttga atctcattgt gagagctcac aaattggaga atctgcccaa gttggcccgt
    44101 acacccatct gcgcagtgga acagtgcttg cagagcaggt tcgtgttggc tcatttgtcg
    44161 agattaagaa ttcaacaatc ggagcagcat ctaaagttcc acatctttca tatgtgggag
    44221 atgccacaat tggaacagat actaatatcg gcgctgcaac agtgattgtt aactatgacg
    44281 gtgttgaaaa acaccagacc gtaattggtg atcatgtaag aattggcagt gattccatgt
    44341 tggtggcgcc agtcagtatt ggtgatggcg catatacggc agctggctca gtaattaccg
    44401 aagatgttcc tcccggagcg ctagggatag gaagagctcg acaatctaat atcctcggct
    44461 gggtattaaa gaggagaaaa ggcagtaagt cagcagaagc ggcagccaag aaagaagggt
    44521 cgaaatgagt aacgaactac gtctcagctc cgaaaaacgt ctccgtctct ttaccggacg
    44581 tggataccct gaattagccg atgaggtggc atcagaactt ggtattccaa ttacccccac
    44641 atctgcatat gactttgcca atggtgaaat atttattaga ttcgaagaga gcgttcgcgg
    44701 ctgcgatgcc ttcgtaattc aaagccatac cgctccagta aataagcaga tcatggaaca
    44761 actaataatg gttgatgccc ttaagagagc atcagctaag cgaattactg ttatcgcccc
    44821 cttctatggc tatgccagac aagataagaa acatagaggc agagagccga tatctgctcg
    44881 tttaatggca gatttattta aaactgcagg cgccgatcgt ttaatgacag tggatcttca
    44941 cacctcacag attcaaggat tctttgatgg tccagtagat cacttatttg cgctaccact
    45001 tctagcaaat tacgttggtt caaaagttga tcgctcaagg ttaactatcg tctctccaga
    45061 ctcaggacga gtgcgagtgg cagagcgatg gagcgatctg ttaggtggag ctcagattgc
    45121 ctttattcat aagacacgtg atccaagaat tccaaatgaa gcaattaccg gacgcgtagt
    45181 tggagatgtc tcaggacaaa cctgcgtagt tatcgatgac atgatcgata ccgctggcac
    45241 cataactaaa gcgatagatg cgctctatga cgccggtgct aaagaggtaa ttgttgcggc
    45301 aactcatcca gttttctctg gccctgcagc agagcgccta gctaactcca gagtaaaaga
    45361 agtagtagtt gtaaatactc ttccgatacc tgaggaatca agatttccaa ctctgactgt
    45421 tctatctatc gcacctcttc taggtagagc aattcgtgaa gtatttgaag atggctctgt
    45481 tacgagcctc tttgatggcc attcttaaaa gagcaaggct gcgctggctc tctcttcttc
    45541 tctgcgcatt ctttctactc ccacttacac ctgcccaatc aaatgagccc tatcgcataa
    45601 cagtgatgtc aagaaatatc tatcttggcg cagatgttgg ggtggcactg gatttgattc
    45661 ctaattttcc agccgcagct caatttatgt gggatcagat gaagcagaca aatttcaaag
    45721 caagagctcc aaaattagcc aatgaaatcc tgcaagatcg cccagaaata ataggaattc
    45781 aagaggcaac ttcttggcga tgcaagaaag atttttttgg cccagaagtt gaaatctata
    45841 acttccttga tgatttgata gctgctacaa agagtgcggg actttcttac tccatagcct
    45901 ctgctaatgg cgtcaaagcc tttaatccag gctattcaat cggtgctatt ccctacctaa
    45961 ctaaagtagt tgatccagaa gtttttaatc caatatttgg ccaagatagt gcggcctgcg
    46021 gctttgttat tggcgatgct ctcttagtgc gagatgatgt gaaatctcag ataatccaag
    46081 ttggaaatag cgaatacgaa gcaagttatt ccatagttcc agtattgatg aatatctatc
    46141 gtggatatag ctgggctgat tttaaagttc aagactcagt ggttcgggtt attacaactc
    46201 accttgagtc aatctgggat gagaataaga ttccaaattc tgctattcag gctcagcaat
    46261 tagttgctga tctaaaagat gccaagatgc caattatcat ccttggcgat ttcaatgccg
    46321 attatcgaga tccaagagga gttgatgaac ctaacccagg tgagcaacca gttgttagtg
    46381 atacttgtcc aacttctggt ggagctaagt gcaacgctta ctcgacgatg attgaagctg
    46441 gatttgaaaa tgcttcacct gatgctaaaa acgcgcgcta cttcacttgg ggagcatcag
    46501 ctcttctaga tggccctgat aagaagcgag caaagattgc taagcaactt ggtaatcaat
    46561 atggatttac agatcgtcta gattatattt tcactaaaaa tgtttatgcc acagtaagtt
    46621 caaagataat tggcaatgtt tggccagatg gatccggagt gtggaattgc ggtagcaaga
    46681 tttgtttccc ttcagatcat gccggagttg tttcaaccat cgaattacca agaatggcag
    46741 gagcgatcga tccagatcta gatagccatg caaaatcagc tttcacacca tggtatttgg
    46801 cagcaggtgt aattctgcta attcttatct ggcgtattgc tcgtcgtttc aggcggtagt
    46861 tgagcccggt tttgcgcttc taattgcctt taggtagcct tcgcatcact taaggcgagg
    46921 gggaatcccc cgttatcgac gaaaggcaag aaaatggctg aagtttcaat taaaggcgcg
    46981 ctccgtagtg aattcggaaa aggcgcatca agaagaatcc gtcgcgatgg cagcgttcca
    47041 gcagttatct atggacatgg tgagaagcca atacacatct ctcttccagc acgcgaagtt
    47101 ggagtagcaa taaagacctc taacgttctt ctaaatgttg atttaggtga gaagcaagag
    47161 ttagtgcttc ccaagtcaat cgttcgtaat cctcttaaag gaactttgga gcatattgat
    47221 ttaattattg ttcgtcgtgg tgagcgcgtt gtagttcacg tgccagttca cacccatggt
    47281 aagtacgatc cagaaggtat tttggagcac gtacataaca ccattgaagt tgaagtcgat
    47341 gtaacagcaa tcccagcatc acttgatctt gatattgaag gcttgatggc aggagattca
    47401 aagacagcat cagaggtaac acttcctgaa ggtgtaatcc ttaagagtga tccaaagatg
    47461 actgttatcc atctatcggt tcgcgtggcg tttgaagaag ttgcccctgt cgctgctacc
    47521 ccagctgaag gtgaagctgc ggccgctgct gctgctcctg ctgaaggtgc acctgctgag
    47581 gagagtaaag aaaaataaag ggctaacctt gctccatgtt tggcagatca aacaaggata
    47641 aaggtggagc ttctcctaac cgatggctat taattggcct tggtaatcct ggcaaagaat
    47701 atgaaaagac gcgccataat attggtgcag ctttagtatc agaatctgcc aaaaaggctg
    47761 gagttaagtt ttcatctcac aaatctcgtg cagatattgc tgagattagc cttggagttg
    47821 gcgctcaacg agtcttgcta attgcagcaa cgcttcgctg ctatatgaat gaatctggcg
    47881 gtccaacaag ctctcttgct aacttcttta aggtaagtag tgatcacata atcatcgctc
    47941 acgatgagtt agatataccc tttcaatcta tccgaattaa atatggcggt ggcgataatg
    48001 gccataatgg ccttaagagc gtcacctctg gactatctag ctctgattac tatcgcatca
    48061 gacttggtat tggccggcca atcggcgagc aagatccagc agattttgtt ttaaagccat
    48121 tctcatctgc ggagcgaaaa gatttagatc tattcctgca aagaggtatt gatgccgttg
    48181 agcttcttat tacgcaagga attgagaagg ctcagaacgc ctttaataag taatcaacca
    48241 gtatttctaa gtcctgctgc cacgccatta atcgttaata gtaatgctct agtgtttaag
    48301 ggatcactgc taccttcacg gactttcttt agcagcgaaa tttgtagtag atgtagggga
    48361 gcaaggtagg cgtcacgaac ttgaagagtt tgagcaagaa tttcttgatc ggccaaaata
    48421 tctttcttgc cagttagtag caatatctca ttaaccgtca attcaaactc tgccttgatg
    48481 tcatctaaga agtaatgaag tgagggatca actaaggcca aaacatattt actagccatc
    48541 tctaaatcgg ttttagcgat ggtcatctcc acattgctta taaatgttct aaagaaatgc
    48601 caatctttta gcatcgctgc catgctcgat gagtggcctg ctaaacgagc ggccttaagg
    48661 ccacttccca ctccatacca gccagggaca atctgtcgtg attgagtcca accaaatacc
    48721 caaggaattg ctcgaagtga tgacaaatca gcgctggcat ccggccttct tgaggggcga
    48781 gagcctaaaa agagatttcc caactgttcg acaggagttg attgataaaa ataattagca
    48841 agctctgggt tttcaactag cgagcgatat ctactaaagg ctgcagagct aattgattcc
    48901 atcgctgaat tccacttggc aagatcttct ggagattgac gagcagagcg attaagtatc
    48961 gttgcctcta gagctgcagc aagggttaat tcaacatttt ctcttgcaag agctggaagt
    49021 gcgtacttat ctgagatgac ttcgccttgc tcagtcattt ttatctgtcc atctagagtt
    49081 ccccaaggaa gagcgatgat ggcgtcataa gttggtccgc cgccgcggcc gacagatcca
    49141 ccgcgaccat gaaatagtct taacttaacg ccatattttc cagcaacgtc tcttagtttt
    49201 cgctgcgcct gatggatctc ccactggctg gtagcaatac ctgcatcttt atttgaatca
    49261 gaatagccaa gcattacctc ttgaatatcg ccgcgtaatt tcacatattg gcgataaatt
    49321 ttgttattta aaagtttttc taaaatatct ccagctgctc taagttcggc aacagtttca
    49381 agtagaggtg caacatcaat atcagccttt ccactttcaa tatctacaag tccagcttcc
    49441 ttagctagat atagcgcggc caagacatct tcatggcctt ttgtcattga gactatgtag
    49501 gtttcaatta ccgatggatc aaaacttgct tgtagctctt ttatggccac aaaagttcta
    49561 agagtctttc catctatctc gctcagtttt gaactatctc gcctagcttg agtgagttca
    49621 tgggtaagaa aatcagcttt ttcttcaggg gagagttgga gataattagt ttggctaaag
    49681 agcgattgaa ttgcattggc gtgagcctga gagtgctctc taacgtccat agttgcgtga
    49741 gtcagaccaa atgctgagat tgttcgtatg gtgcgctcca gtaagccttt ggctattaac
    49801 tcgccacgat tttgcattag tgagtcatac atcaacatca aatcatcaat tagttcttta
    49861 gtatttgcat aatcacgccc tgcttgatgc tcggtgcgat tttggtgtct acttctagtt
    49921 aaaagcaagc gatgaccaat tgcagtggct ttgagtcggt aaggctcttc aacatttatt
    49981 cttctaaaac gaggctcaat ttctggcagg ttctctaaat ctttagcaac agaatcaagt
    50041 agttgctttg atgttccagc aatcttggtt gaaacagata aggcctgacg aagttcatcc
    50101 attacttcat ttagaactct tatggcatgg cccatctgga gcacaattgc atctttggtg
    50161 acctcaggag ttatgtttgg attaccgtca cgatctccac caatccaaga accaaacttt
    50221 agaactgaat ctcttggcga aacagttatc ccaagccttg aaatttcccg agagaagtct
    50281 tcaagaactt caggaattgt tagtcggaag agatcatcaa ggtagtagag agcattaatt
    50341 gcctcatcaa gtggctctgg tctaccaagt cttaactcat cggtttgcca gagaagatct
    50401 accgattcag caagacgtcg gtcacgagtt gggctatcac ttttatctaa taactcagag
    50461 atagtcgaga gttttccaag tactgagcga cgagcagctt cagttgggtg cgctgtaaat
    50521 actggtcgaa cttgaaaatt cttcaaccac ttttcaatat cttgactagt aaagccttca
    50581 gagctcttac cggcagccaa aatattatcg acagccctac ttaaccagga gccaccttta
    50641 atacgctctt ttgccagaat tcttgagcgg tgaacttgtt cagcaacatt tgctaaattg
    50701 aagtagacat taaatgccct tactagtttt actgattgat ctgctgatat tgattgaagg
    50761 agatcttcgc ccccaccttc gcgcactgac tttctaacca gctccactaa atcaagaagc
    50821 tcttgaccat cttggcgagc tagagattgg cctaatagat cacctaaacg gcggacatca
    50881 cttctaagtg agagattttc ttcggcagaa gtaggcacgg acacgggcta ataatggcac
    50941 gatagaatta caccgcgttt tacagataag cccctttcat cttggaagca gcacccctgc
    51001 cctctatgaa atcggggcta atcggcgttg agcgtaattc aagagaagag ggagcaaatc
    51061 aaagtgagtg gaaagctagc gggctttatc tcgctatctg aaacaacaga gcttgccttg
    51121 gagcagagtg acgttgcagt aattgcggca gatccagctc ttccattcct aattgctgaa
    51181 agaagtaaaa aagctccgca gttagtgatc actcattcaa gctctagggc atcagagatt
    51241 gctctgcaat tatcagagtt agtagatcag gtagcagaat ttccagcttg ggagagtttg
    51301 cctcatgaac gcttaagtcc caatagcgat actgtggcaa ggcgaataga tactcttata
    51361 aatctagata aaactaaagt tgtagtaaca actgcaaggg cgctactaca acctattaat
    51421 caagaaataa ttgaaatgcc gatgttaagt attcaaagcg gcaggcacca gaacttttca
    51481 gagttgattc aagaattgac ccatcgcgcc tataaccgtg tagatatggt ggagcggcgc
    51541 ggagactttg cagtaagagg cggaattatt gatctattcc caccgcttgc agaccatcca
    51601 gttcgaattg aattctttgg tgatgagata gaggagatca agtattttga agttagtgat
    51661 caaagaacct ttgcattagt agacggaagc cttaatctca ttccttgtag agagttgatt
    51721 cttactccag aggtagcaga gcgagcacag aaattagcaa ttaaatatcc agagattact
    51781 gaaatctgta acaaggctgc agagggaatt tatagtcaag gccttgaatc attactttca
    51841 gtcttatcta agaagttggt gccgctactt gaactccttc ctaaaggcta tgaagtaata
    51901 agtcttgatc aagagcgaat tgctctgcga gttagagact taattagcac taatgaagag
    51961 tttcttgaag ctgcgtggtc tagcgctgcc ctttctcaag gagatgacga aagctttaac
    52021 acaccgcttc gccaggagct atctactggt ggctttttag aattaggcga agtcacttca
    52081 tacgccgcag ataatggaat tatttggcgt tatatcaact catacggcac tccagaagat
    52141 ctacagatca atcaatttat cgcagtagag ccatttaaaa ataattttga aaagcttatc
    52201 cagcaagtta aaacttggat tagccaagga tttttagtgg tgatctcctt ggaaggtatt
    52261 ggcattcttg agcgttatcg agacatattt gcagatggcg atattgcagt tgctctagtt
    52321 gaaaagttaa gttctgatct attgccggat aagctctatt taacttctac tgtaatgcgc
    52381 catggattta ttgatcaaga gcagaagatc gtctttctta ctgaagctga tatcactgga
    52441 aataaagacc tgcgcgcagc aacttcacgg atgccatcca agagaaaggc aagcattgat
    52501 cctctcgagc taaagagcgg agattatgtg gttcacgaac aacacggcgt tggcagatat
    52561 cttgaattag tgcagcgaga tgttgcagga atatccaggg agtatttggt aattgaatat
    52621 gcctcttcaa agaaaggcca tcctgcggat cggatttatg ttccaactga ttctctagaa
    52681 cagattacgc gctacatcgg tggtgaagct ccagcagtac atcgaattgg cggcggggag
    52741 tggatcaaag ctaaaagtcg agctaagaaa gcggttaagg aaatcgctgg cgaattgatt
    52801 cgtctatatg cggcaagaac tagctctcct ggatttgctt tctcacctga caccacgtgg
    52861 cagcgagaac tcgaggactc ctttgcctat gttgaaactc cagatcaatt agtgactatt
    52921 aatgaagtca aagaggatat gcaacgcccc tacccaatgg atcgaattat ctgtggcgat
    52981 gtgggatatg gaaagacaga gattgccatt agggcggcat ttaaagcagt tcaagatgca
    53041 aagcaagttg cagttttagt tccaacaact ctacttgctc agcaacacct ggcaacattt
    53101 acccagcgct atagtggatt tccaataact gtttcagctt tatcacgttt tgcatcaagt
    53161 aaggagataa gtgaaacgct agccggcctt gctagtggcg gcgtagatat cgtcattggt
    53221 actcatagat tgctctcaga tgatgttgct tttagagatt taggtttgat aattgttgat
    53281 gaagaacaaa ggtttggtgt tgagcataaa gagaaattaa agaaattgag agcaagtgtt
    53341 gatgtactgg ctatgtctgc aactccaatt cctagaactc ttgagatggc cattacaggt
    53401 attcgagaaa tgtccacaat aaccacgcca ccagagcagc gccatccagt tcttacctat
    53461 gttggcgcat atgatgaaaa gcaagtagca gcagcgattc accgagaact tctgcgagat
    53521 ggccaagtct tctatattca taatcgcgtg gaatcaattg atgatgtggc tgccaagatt
    53581 agaagattgg tacctcaagc aagtgtggct atcgcacacg ggcagatgag tgaagcaaat
    53641 ctagagcagg tagttgtagg tttttggaat cgagaatttg atgttttagt ctgcaccaca
    53701 attgttgaaa atggtattga tgtcgcaaat gctaatactc taattgttga gcgtgcagat
    53761 ctctttggac tatctcaact tcatcaactt agaggaagag ttgggcgctc acgagagcgt
    53821 gcttatgcct acttcttcta tccaatcgat agagcgatga gcgaagtggc acttgatcga
    53881 ttaagcacaa ttgctcagaa caccgaactt ggagctggaa tgcgcgtggc tcttaaagat
    53941 ttagagataa ggggggcagg aaatctatta ggcggcgagc aatctggtca cattgcagat
    54001 gtgggatttg atctatatat gcgcatggtc ggagatgctg tcaatgagta caaagcagga
    54061 tttatagatg gcgggcaaga attaactgat tgtaaagttg aattgcctgt caccgctcat
    54121 ctctctgctg aatatgttcc atctgatcga ctccgtcttg atctatatcg aagacttgca
    54181 gatacaaagg atgaggatga gatcacaaag atcggtgagg agttaagtga tcgctttggt
    54241 gctcttccta aagaggccga gaatttgctg cgcatcgctc gactacgcac gtacttaaag
    54301 gagcgaaaga ttcaagactt tgcagtgcag ggccgctacg taaaaattgc accacttctg
    54361 ccaagtgaat cgttagagtt gaaaattcag cgcctctatc ctgggtcaat tgtgaagtcg
    54421 gttacccaag ttgtgatgat tgctcgtccg caaactgctg cctgggtatc agaggcggaa
    54481 gaaatagggg atacttctct cattgattgg gcggttgagt tagccaaaac tctccttgag
    54541 cgccccttag gaaagtgaga atcctgtgaa gaagtttcta gcacccatta tcgccatatt
    54601 ggcgctagct ctatcttcct gttctcaggt cgattcagcg gcaactattg gagatgagaa
    54661 gataactatt gctgcgctac aagagcaggt tgatttaatc cttgcagagc gcgaaggtat
    54721 tgatacaact cagatgagtc ttgagagcgg agaagcttta actcgctcac aactttccta
    54781 catgatctca aatctaatta tcaatgaagt tgccaaagaa gagaagattg aaatagatca
    54841 agcagaaatt gatgcctaca agattgagat ctatgccaat atcggaggcg aggaaaacct
    54901 tccgaatgta cttgtgagcg cttcaattcc ctcaacatct ctagataatg tccttcgtag
    54961 agatttaatc cttcgcaaga taactgatgg cgcaatcgca tcgggagtta gcgaagccga
    55021 agttaatgac ctaattcaga aaattgtcac tgataaagca acagcattga gcgttgaagt
    55081 taatcctcga tatggcacat gggatgcaac aagcttaact gtcgttgcta aagagcctgc
    55141 gggcgatgca gttaatgata agtaagtaaa gctctgcaat atggcgtatg aaaatctagc
    55201 tcgacttgct caagtgatgg acacccttag atctcctggg ggatgtcctt gggatagtga
    55261 gcagagccat caatcgctac ttaaatattt acttgaagaa tcttatgagt ttattgaagc
    55321 cgttgagagc ggtaatcgcg aagatatgcg tgaggagttg ggggatattc tcctgcaagt
    55381 ttatttccac tcaagaattg ctgaagaaca tgaagaaaag cccttcaaca ttgatgatgt
    55441 tgctaaagca ataatagaaa aattgatctc acgccatcct catgtcttct ccgatgccaa
    55501 agtagattcc agcgctgagg tattggaaaa ctgggaagag attaagcgca aagaaaagtc
    55561 tagatccagt gctcatgatg gagttccaac tggtcaacct tcattagctc ttgctagcaa
    55621 attgatttat cgggctagca agaatgacct ttcaacacca gagcatccga aagaaaagat
    55681 cgaagtaagt gaagctgcgt taggaaatca actcctatcc ctaatctcat gggcgatagc
    55741 aaacaatctt gatcctgagg ttgctctacg caaggctgca ctcaaatatc gtgatgcaat
    55801 gagtgaagaa gaatcaggct agacttcgcg cctgtgagca caaattcagc gattaaatcc
    55861 atcaccgcaa gagagatcct tgattcaaga ggaaatccaa cagttgaagt agatgtccaa
    55921 ttagaagatg gttcttgggc gcgagcagca gttccaagcg gagcctcaac tggagctttt
    55981 gaagccgcag agttaagaga tggtggctca agatatttag gaaagtcagt atctggagcg
    56041 gtagaaaacg ttagaacaaa gattgcgcca gctttaattg gtcaggatgg cttagaccaa
    56101 aaaggcttag atgaaaagat gattgcactt gatgccacag ttaataaatc atcacttggc
    56161 gcaaatgcca tccttggagt ctcgctagca gttgcaaggg ctgctgcggt aagcaagaag
    56221 ctaccctttt ataaattcat aggcggagaa aaagcaacac ttctaccaat tccaatgatg
    56281 aatattttaa atggtggagc tcatgctgat acaaatgttg atatccaaga atttatgatc
    56341 gcaccaatcg gcgctccaag ttttaaagag ggcttgcgct acggagctga gatatatcaa
    56401 tcactcaagt cagtattaaa aaagcgcggc cttgccacaa gcattggcga tgaaggtggt
    56461 tttgcgccaa accttgagag taatcgcgct gcacttgatt taattattga agctgtagaa
    56521 aaggccggct acaagatggg aagtgatatt ggccttgcga tggatgtggc tgctacggaa
    56581 ttctataaag atggtagtta tcactttgaa ggttcaaacc taagcgcaac tgagatgatt
    56641 aactattatc aaggattatt ggactcctat ccattgttat caattgaaga tccactctct
    56701 gaagatgatt ggagtggttg ggccgatata acaaaggcaa tgggagctag cgttcaatta
    56761 gttggcgatg acctatttgt cactaatcca actcgtctag ctcaaggaat tagccaaggt
    56821 tgtgccaatg ctctgcttgt taaggtcaat caaattggga cccttactga aactttagct
    56881 gctgttgata tggcccataa agcgggatat cgctcgatga tgagtcatcg ctcaggagaa
    56941 actgaggaca ccacaattgc agatttatca gtggcagctc tctgtggtca gatcaaaaca
    57001 ggtgctcctg ctcgtagcga gagagttgct aagtacaatc agttactgcg aattgaagag
    57061 gaactgggtt caaaggctca atatgccggc cgtgacgcat ttcctcgttt tacttcgcgc
    57121 taagcaataa cttagagcaa ataaggagcc gctatgaagc gcagcaaaat ctcgcttcct
    57181 aagactcttg aaagttttgc cgcgaaacga ggaatctcaa ctcgattatt aattctcttt
    57241 ctagttctat ttattgtcgc cgttactctt gctcctccaa cgcagcagta ttttgcgcag
    57301 agagcgcaaa taagcgccct tgaagcagat ctagattcaa attataaaaa gcttgaggca
    57361 gcaagagctg agctacaaag atggcgtgat ccagattatg taaagagtga ggcaagaaat
    57421 cgactccact ttgttctgcc aggtgagcgg caatacataa ttttgggagc aaaaccaaca
    57481 attgaccctg aagcccaagg agctgctcca atcaatcaag actttccatt aggagttccc
    57541 tggtattcca gagttatttc atcaattacc tcagtcggag tcggcgtaac tacgccgtaa
    57601 tctttattta tgggtgagag agcaagcgat gaggatctat ctgcaataga gattcaatta
    57661 ggcagagtgc caagagatgt tcatagcatc gcctatcgct gtgcctgctc acatccagct
    57721 gtagttgaaa ctaacccacg tctttcagat ggaacgccat ttccaacctt ctattacgcc
    57781 acctgcccta atctcactgc tgccatttca actctagaaa actcttccct tatggaggag
    57841 atgaatgagc gcttagcaat tgatgccgaa ctatctggtc aatatcgagc agcccatgat
    57901 gattatttgg cagcaagagc tgcccttggt atcgatgtcc cagaaatcgt tgatatctca
    57961 gcaggtggta tgccagatcg tgttaagtgt ttgcattcgc taattgctca tagcttggca
    58021 gcaggggagg gagtaaatcc cttaggcgat gaagccctct cacttcttcc aaagtggtgg
    58081 ctaaatggaa attgcttaga acggcgtgat cagtgagtcg agtagctgca attgattgtg
    58141 ggacaaattc tatccgtctg ctaattgcag atgtttcagg aagcaatcta cgtgagattt
    58201 atcgaggaat ggaaattgtc cgccttggac aaggagttga taagaacaaa tcttttgatc
    58261 ccgatgcaat agataggact ttgagagcta ctgaattatt tgcaagtgag attagaagac
    58321 gtggcgttga gtcaattaga ttttgtgcca caagtgccac tagagatgca tcaaatagag
    58381 agctcttcat agatggcgta aaagcgatac ttggaattga acccgaggtc attagtggag
    58441 aacaagaagc tgagctctcc tttatcgggg caactcatca gctaaatcaa ataggtgctc
    58501 cctatttagt agtagatatt ggtggaggct caactgaatt tgtctatgga cagagcacag
    58561 ttgaggcatc ggtgagtgaa aatattggct gcgtgagaat gagcgagcgc cacttaaata
    58621 gctctccgcc tagtaaagct gcgattgatt tggcaattac tgatattgat agcgctatta
    58681 aaaacgctgc tacaaaagtt ccaatagcaa gtgctaaaac attagtagct gttgctggaa
    58741 cagcaaccac ggttgcagct gccgcacttg gccttgaaag ctacgaccgc gacttaattc
    58801 atctctctcg aatcagcgca gaaaagactc atcaagtctg tcagagcttt ctcgcacttg
    58861 agagatcaga gattgcatca cttggctata tgcatccagg gcgagttgat gtaattgctg
    58921 caggatcttt agtgctttca agaattatgg cacttagtgg ggcaagtgaa tttgttgcct
    58981 cagagagtga tattttagat ggaatggctc ttcttcttgc gaagtaaacc cagagttata
    59041 gccaaggata ttaagagctt aaaagttctt gataaagaga ttattaaatg cagcgcctgt
    59101 ccaaggctga catcttggcg caaggaggtg gcaattacca agcgcgctgc atatcgcaat
    59161 gaagattatt ggggaaaacc agttactgga tttggtccaa gtaattctaa gttattaatt
    59221 gtcggacttg ctcctggcgc tcatggggca aatagaacag gaagagtatt tactggagat
    59281 tcttcaggag attggctcta tagatccctt cataaaaatg gtttagcaaa gatttcaact
    59341 tcactatcaa gtgatgatgg acaagagtta tttgaaactc gcatcacctg cgcgcttcgc
    59401 tgcgctccac caggaaataa accaacaaca attgagcagc aaacctgtaa gagctggctt
    59461 gaaacagaga tgaaattact tatgccaact ctgcgcgtct ttctgccttt gggccagatt
    59521 gcctggaagg caacgatcga tgccttgctt gcgataggtg aagaattgcc gagaaaacgc
    59581 ccaaagtttg gccatggggc tagtttcaac tttcttggcc gcgacgggaa tactagattg
    59641 gtcatcggta gttatcaccc gagccagcag aatactttca cgggaaagct cacgcgctcc
    59701 caactggatt cggtggtaaa aaaggcaggt agatttgccc acgcggaagt ggcccctgta
    59761 gcccaacggc agaggcagac gacttaaaat cgtccaagtg tgggttcgac ccccaccggg
    59821 ggcacgcatt aagaaaaaaa agttcataat gggtagttag aactacaaaa ggagagagat
    59881 gaatagttcc aacccagtac ttggtaaggc atttggcgag aagggccgta gaggttatgc
    59941 cgctatggat aacttcccgc aagataatct agagcagagc tttaacgctc ctgctgcttc
    60001 atcacttcgc actggtcgta tgactatgga tgatgttgtt gcaagaagcg gaatgctctt
    60061 tggaacctca gcacttgttg gcgcagctgc ttggtatttc aatcttggtg gcggcttact
    60121 tcttcttggc tttgttggtg gattagtaac ggccatgatt ggaattttta gtaagacgat
    60181 tcgtcctgga gtttatttag cttatgcagt ctgccaaggt ttagttcttg gaataattag
    60241 caagacctat gaactcttct atcctggaat tgttcagcaa gcaatcgttg caactgctgc
    60301 agcatttatt ggaatgctta ctctttacaa gagtggacgc cttcgcgtaa ctcctaaatt
    60361 caccagaatg cttcttggtg ctgcaattgg ttatctagtt ctagcacttg gaagcttaat
    60421 tggatcattc tttggacttg gcggcggagc aggactatat ggtctcagcg gatttggacc
    60481 tcttcttgct gttgcaggag ttgccattgc atccttcttc ttaattcttg actttgatca
    60541 aattgaagag ggcgtaagag ctggagtgcc acaagaggaa tcttggcgag ctggctttgg
    60601 actattgatg actatggtct ggctatatct agaggtattg cgcttgatct caatacttcg
    60661 cggaaatgat taattagacc tcatttatag gaggtttcct agaatccccg agatgggcag
    60721 aatttgtctc ggggattttt agcgccacaa taattgcaag tgaaaacact gcagcgctca
    60781 ctcctatcgc tgttcgataa gaaaaagcat cagagataaa gccaagtaat accggtccaa
    60841 ccatcattcc ggcatctcct gccatctgcc agaaggcata gacctttcca ctctttcctt
    60901 tgattacatc gccaacaatt gcgccagcag atgcag
//
LOCUS       DAOROD010000006        54392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_18_length_54392_cov_29.309108, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000006 DAOROD010000000
VERSION     DAOROD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 54392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54392
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_54392_cov_29.309108"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..74
                     /locus_tag="PLW53_02420"
     tRNA            3..74
                     /locus_tag="PLW53_02420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Gln,seq:ctg)
     gene            118..193
                     /locus_tag="PLW53_02425"
     tRNA            118..193
                     /locus_tag="PLW53_02425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:152..154,aa:Glu,seq:ctc)
     gene            complement(198..917)
                     /locus_tag="PLW53_02430"
     CDS             complement(198..917)
                     /locus_tag="PLW53_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRD36311.1"
                     /translation="MDKKNSGVGVLDKAVKILSVLESGPHSLSELVAATKISRPTAHR
                     LAVALEHHRLLSRDLTGRFVLGVRAGELAASAGEDRLLAAANPALIALRDATGESAQL
                     YRRQGEMRICVAAAERLSGLRDSVPIGAALTMSAGSAAQILLAWEDAEKLHRGLTGAR
                     FNAAALAAVRRRGWAQSVGEREAGVASISAPVRGPNNRVIAAVSISGPIERLGRQPGR
                     IHAAALVATADRLSEYISKKN"
     gene            981..2378
                     /gene="leuC"
                     /locus_tag="PLW53_02435"
     CDS             981..2378
                     /gene="leuC"
                     /locus_tag="PLW53_02435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRD36312.1"
                     /translation="MAKTLAEKVWDDHLVRSASGEPDLLYIDLHLIHEVTSPQAFDGL
                     RLAKRGVRRPDLTLATEDHNVPTLNIDKPIADPVSRLQVDTLRKNCQEFGIRLHSLGD
                     VEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPL
                     SRPKTMAINVEGKLKPGVTSKDIILAIIAKIGTGGGQGYILEYRGSAIRALSMEARMT
                     ICNMSIEAGARAGLIAPDETTFAYLKDRPHAPKGEDWQRALAYWSELKSDSDAKFDKE
                     ITLDADQLSPFVTWGTNPGQGIPLDGVIPSPQDFTDPEEVSAAERALVYMDLKAGTPM
                     KKIKIDTVFLGSCTNGRIEDLRAAAEIIKGKKVASDIRMMVVPGSARVRLQAEAEGLD
                     KLFLEAGAEWRSAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVSPLVA
                     AATALRGTLSSPADL"
     gene            2390..2989
                     /gene="leuD"
                     /locus_tag="PLW53_02440"
     CDS             2390..2989
                     /gene="leuD"
                     /locus_tag="PLW53_02440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD36313.1"
                     /translation="MEKFISHTGSALPLRRSNVDTDQIIPAVYLKRVTRSGFEDGLFA
                     AWRSDPEFVLNKAEYSKASILVAGADFGTGSSREHAVWALQNYGFKVVISSRFADIFR
                     GNSLKAGLLTVILGQSDVEALWSAIEAEPSLEIKVDLETRLVSYKNQAISFDLDDYTR
                     WRLMEGLDDIGLTLRKIDEVENFEEKRAKFKPKTLPVLS"
     gene            3182..3448
                     /locus_tag="PLW53_02445"
                     /pseudo
     CDS             3182..3448
                     /locus_tag="PLW53_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            3652..4449
                     /locus_tag="PLW53_02450"
     CDS             3652..4449
                     /locus_tag="PLW53_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD36314.1"
                     /translation="MSVVRPEISYLPPQGDPLGVNPWFRFGAAVLKPILNLIIKKDWK
                     GAQHLPMSGAAIVVCNHLSYVDPLTFTHFLYNNGRAPRYLGKESVFRIPIIGRVIRGA
                     GQIPVSRESKDAVKGLEHAIAVLKAGHLLGVYPEGTLTRDENLWPMKAKTGVARLALI
                     TGAPVIPCASWGPEKVLPPYSKRLRLFPRSKVSILMGPVVDLSPWQGKSDDLEAVEQA
                     ADHIMDRITELLEILRGQKAPAIRFDPKNSDLPRIGNFKKAKKDKSK"
     gene            4446..5450
                     /locus_tag="PLW53_02455"
     CDS             4446..5450
                     /locus_tag="PLW53_02455"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRD36315.1"
                     /translation="MKIAVLGSGQWGSTLAQVLIDAGNDVIIWGRNNEVVAEINSKHQ
                     NQSALGEHLLPEAIYATSEIKVALEGAQLLVLAIPSQSLRQNLELWKGLIGPDIPIVS
                     TLKGIEISTQLRMSEVICQVLGRDESSIAVLTGPNLAREVILRQPAGAVAASTNQKLA
                     ELTSKAFSAPYFRVYTSNDVIGCELASATKNVIAIAVGMSIGMGFGENTQALVITRGL
                     NELTRLAIARGAQPLTFVGLAGVGDLLATCGSPLSRNRTFGEALGRSGSVEQAQKAIS
                     STAEGLTTAQALVELAHLVGVEAPIMEAVCDVVTSKISPQQAFLNLMKIQTGAEIDHV
                     "
     gene            5462..6514
                     /locus_tag="PLW53_02460"
     CDS             5462..6514
                     /locus_tag="PLW53_02460"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRD36316.1"
                     /translation="MAIVAGGQSSEHPISCISASGVLAAIDKSKFEPILIGITQSGTW
                     IDMRESDFVKGSDGLSYVPEQGEKLVVDVHGIKDSKGNDLGIDLVFPLLHGAYGEDGT
                     IQGLFEMAGIAYVGSGVLASAVAMDKSFAKPIYADFGLKVADGITIHKDNWNKSKDLE
                     IAKIRALGFPIFIKPARSGSSRGTSKVKDESAISAALEDAFNYDPRVLVEAAVVGREI
                     ECAVLQIDSVATASVLGEVRVHPPHEFYDFDAKYIDSSTTFDIPANLPESIASAISQA
                     AITAFEALGCEGLARVDFFLSDDNQIIINELNTMPGFTPTSVFPALWQKTGKSYSQII
                     TILLESALSRKKNVIR"
     gene            complement(6523..6759)
                     /locus_tag="PLW53_02465"
     CDS             complement(6523..6759)
                     /locus_tag="PLW53_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRD36317.1"
                     /translation="MSIQAYILIQTEVGKAASVTKAVNAIAGVTIAESVTGPYDVIMR
                     AEASSMEDLGRSVLAKVQAVPGITRTLTCPVAVL"
     gene            6901..7830
                     /gene="thiL"
                     /locus_tag="PLW53_02470"
     CDS             6901..7830
                     /gene="thiL"
                     /locus_tag="PLW53_02470"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRD36318.1"
                     /translation="MREDEVLKNVRAIFDYGQSDSALIVGNGDDGAVLGPRSELTVLA
                     SDMAVEGIHFNFAWSSAMQVGRKISAANLADICAMGGWPEYFLVALAFPPSFSGQVLE
                     LAKGIKTECQRVGVKVIGGDLSRSNEVVISISAIGRVKRPILRSGAKVGERLFISNLP
                     GFSQAGLSLLKSSSDNQSPAANRARMAHLAPDLDYEKYRKSYEYLSSAIDTSDGLVID
                     ASRIAAASGVGIDIRVEALINAPEFSELESASGSRESALESALYGGEDHLLLATSDAQ
                     DFGFLEIGQVIKGDGIYLDGVKTKTSGGYQHSW"
     gene            complement(7841..8026)
                     /gene="rpmB"
                     /locus_tag="PLW53_02475"
     CDS             complement(7841..8026)
                     /gene="rpmB"
                     /locus_tag="PLW53_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRD36319.1"
                     /translation="MAAVCDICGKGPSFGNNVSHSKRRTRRRWNPNIQRVRTLINGYS
                     KRKNVCTSCLKAGKVLR"
     gene            8196..10415
                     /gene="recG"
                     /locus_tag="PLW53_02480"
     CDS             8196..10415
                     /gene="recG"
                     /locus_tag="PLW53_02480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRD36320.1"
                     /translation="MASLDSSLLSILGDRTAEVLDENLGLKTISDLLRHYPRRYVIRG
                     ELTDIESLIEGEEVTIFAKVESSKVKRIPGRKSAILETVVTDGRAKLTLTFFNQAWRE
                     KELRPGRQGLFAGKVGLFKGKRQLAHPDYLLIPEGDDVDQAIGDFAGKFLPVYPATAK
                     MPSWKIAKCVRLALDGLDEIAEYLPQELLFEFNYPKISEAFRQVHQPDSAESAELARQ
                     RLTFDEALLMQLFLVLRRYEVRAAKTTSRAPRNDGVLAAFDKRLPFELTAGQRQVWSE
                     IVKDLSSDHPMYRLLQGDVGSGKTIIALRAMLAVVDSGGQAALLAPTEVLAQQHYRNF
                     LSLLKDLAEAGMIGGNQSGLQVRLLTGSTPAAQRREVLSAISSGQAGITIGTHALLGE
                     SVNFKDLGLIVIDEQHRFGVEQRDALRDKAREPAHVLVMTATPIPRTVAMTIFGDLDV
                     STLRDMPLGRSPITSHVVSQSSKPEHLTRAWQRACEEVGNSNQVYVVAPRISADDESD
                     LQRFGLTPDELILAKNLIDDEDSSSLKLAMSSVEELYPMLTADVMKDLRVGKLHGRLS
                     SEEKEAVMQAFVRHELDVLVTTTVIEVGVDVANATMMIIMNAERFGVSQLHQLRGRIG
                     RGVSPGLCLFVTSASEDSPAMARLKAVAATTDGFELSRLDLQERSEGDVLGSAQSGAR
                     SHLRLLRVIRDEELIAQARLVAENLLSQDPTLSANPELAQAVEKLRQEERAAYLEKR"
     gene            10416..10985
                     /gene="rsmD"
                     /locus_tag="PLW53_02485"
     CDS             10416..10985
                     /gene="rsmD"
                     /locus_tag="PLW53_02485"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRD36321.1"
                     /translation="MRIIAGLAKGRNINSPISQVRPTSDRAREALFSTLESEFGSLSD
                     ANFLDLYCGSGAVSCEALSRGAGVVVAVDNEEKATSVTRSNFELLEKIPGIGSASVIT
                     MSAGKYLDKGADLAFDIVFIDPPYDLPNSDVEKILLSLVNNGFLKSSSIIAVERDSKT
                     KPFSWPQGLAELKVRKYGAASIYYGEPRQ"
     gene            11017..11526
                     /gene="coaD"
                     /locus_tag="PLW53_02490"
     CDS             11017..11526
                     /gene="coaD"
                     /locus_tag="PLW53_02490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRD36322.1"
                     /translation="MKKAVCPGSFDPITNGHLDVIERASHLFDEVVIAVLVNNSKSSL
                     FTIEERIVLARDCVKHLPNVKVDMWSGLLVDYCRENKVDAIVKGLRAVSDFDYELQMA
                     QMNLQLKGVDTLLMATKPAYSFLSSSLVREIARYGGDVSNLVPAGVLSELTRKAKDSP
                     SPSSQSGRS"
     gene            11528..12004
                     /locus_tag="PLW53_02495"
     CDS             11528..12004
                     /locus_tag="PLW53_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36323.1"
                     /translation="MEAIEKLQSAITMVNEARSVPLSASCVVHRGEMLELLEEINQAL
                     PKDLSNAQKLLGSRETIIEEGRQSAEQLIAHAREEVARMVEQTAIVAAAREESARLID
                     EAHSKVEKEREEVDAYIDSRLATLEVILNKTLEAVNRGRERLAGASDKDVLSQLAE"
     gene            12012..12548
                     /locus_tag="PLW53_02500"
     CDS             12012..12548
                     /locus_tag="PLW53_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRD36324.1"
                     /translation="MNSKTESPFKVNFHELPRRAGEFRQYHLQFSAPQAIGIALLEIP
                     KSEPIDISFKAESVDGGVLLTGTVISHAKGECGRCLDPIDFDVNQKFQELFLYSSKVS
                     EVEDDEDEVFSLDGDIADLEVPIRDAVILSMPINPLCQSDCLGLCSVCGLKRRELEKD
                     HAHKASDPRWSGLADWKP"
     gene            12633..12806
                     /gene="rpmF"
                     /locus_tag="PLW53_02505"
     CDS             12633..12806
                     /gene="rpmF"
                     /locus_tag="PLW53_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRD36325.1"
                     /translation="MPLPKRKMSRSRTRSRRSQWKATPASTANCPQCAQPKLQHTACP
                     TCGTYNRRQVLEV"
     gene            12820..13482
                     /gene="rnc"
                     /locus_tag="PLW53_02510"
     CDS             12820..13482
                     /gene="rnc"
                     /locus_tag="PLW53_02510"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRD36326.1"
                     /translation="MTEKLGVSVKPELLELALTHRSFAYEFGGLPTNERLEFLGDSVL
                     GLVITEALYERFPDLDESRLSPLRSGIVNMRALASIARELELGSALKIGKGEEVTGGR
                     DKNSILADAFEALVGAIYLDHGFDVSAEIIMRLMKSAIDEAVTRGAGLDGKTALQEIV
                     ASSGWAPPEYKVSESGPDHDKDFVAYAIVNGVTYPQGHGKSKREAEQVAARIAFEALS
                     NN"
     gene            13486..14385
                     /gene="mutM"
                     /locus_tag="PLW53_02515"
     CDS             13486..14385
                     /gene="mutM"
                     /locus_tag="PLW53_02515"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRD36327.1"
                     /translation="MPELPEVETVRRGLEPFIVGRRIKEIDVIHKGGNRSTTYAPYSA
                     IEGAKVLSVNRRGKFLWFELNRDFALMAHLGMSGQLLIQDKQREYEKHLRVRIDCGDR
                     RKEIRFVDQRTFGWISIDKLVLEGQDLIPQSFTRIAPDLFDKKFDRSAVIGRISKKNS
                     AIKRVLLDQAVVSGVGNIYADEALWRAKIHPERLAKKLSEQEISNLLDATKFVMEKAL
                     KAGGTSFDELYINVNGESGYFDVELEAYGQENEPCSRCGCEIRRIAFANRSSHFCPQC
                     QSKSPLKKAGKKKVRKKVSSKRG"
     gene            14425..17970
                     /gene="smc"
                     /locus_tag="PLW53_02520"
     CDS             14425..17970
                     /gene="smc"
                     /locus_tag="PLW53_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRD36328.1"
                     /translation="MYLKSMTLKGFKSFAAPTTLKFEPGITCVVGPNGSGKSNVVDAL
                     SWVMGEQGAKSLRGGKMEDVIFAGTSGRAPLGRAEVSVTIDNADGALPIEFSEVTISR
                     TLFRNGQSDYQINGESARLLDIQELLSDSGIGREMHVIVGQGQLDAILTANPEERRGF
                     IEEAAGILKHRKRKEKALRKLDSMQGNMSRVQDLTNELRRQLRPLGKQAEVARKAAVI
                     QADVRDAKLRILADDLIAFNSTLSLEVADETALRQRRSEVDQELEQLRKREIELDQIA
                     AIQGPELASAQNNYYSLTAQKEKLRGTQNLASERARFLAEEAEEARASGRDPEALDAE
                     AKVLRGEESVLQGSSDKARAELVAASNSVTQLEARLKGEEDRVAAAQRALADYREGTA
                     RQEGHIKSLQSRIDGAGSEINRLESALTGAKARVAEAKVEFARLESEIAGLDAGELGL
                     DAEFEKAKALLNGSKAGLEKLRSDLSLAERARAASEAKIDALRQATLHKDGSGALLTN
                     DQGIVIRGSLASLLTVEPGWQSAVTNALGSLANALVLQDLNQVISAITFLKSKSAGSA
                     ELLFISEGDSRPVVEIPIGLTPLASKVSSATLNSILPALFSGLVAAESLSQASQIIAS
                     NPSLIAVTRDGDLVGRGRASGGSSARTSLIEISEMIEKAEIELQRHGHDCDRIHFDIS
                     TAETKLRSDQLTYDQALARLNESDAAMAALAEQMAAASQGVKSASGEVERLEKSITEL
                     KQALNNDLSELNIARSEFEKSSEPLEPELSTLENLRATLATARAVEVEARLALRTIEE
                     RVAAISQRARDLELAASNERQAASNNIARRAARARGAVTAQAIADAAYEALIQIESSI
                     SRANSERERLELARSNRDGEILSIRQAIRDLSVELDQLTSSVHRDEIARAEQRLRIEQ
                     LEIKAMEEFGVDSSSLVNEYGPDKPVPTFIEDDAGNLSQGEFIPYVRQEQEKRLAGAE
                     KSLALLGKINPLALEEFSSLEERLKYLADQLEDLKKSKKDLLDIIKSVDDKVQEIFEE
                     AFRDTAREFEKIFARLFPGGEGRLLLTDPSDLLNSGVDVEARPPGKRIKRLSLLSGGE
                     RSLTAVALLVAIFKARPSPFYVLDEVEAALDDTNLGRLLSVLEELRQASQLIIITHQK
                     RTMEIGDTLYGVSMRGDGVSEVISQRLREIETEPA"
     gene            17973..18809
                     /gene="ftsY"
                     /locus_tag="PLW53_02525"
     CDS             17973..18809
                     /gene="ftsY"
                     /locus_tag="PLW53_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRD36329.1"
                     /translation="MGLFGSFLSKFSKSKATIEDLEQFRQILISSDIGVGITEEILDL
                     VKREKSEELSTAIASSLKSKLTTSSRVINLSSDGPTVILIVGVNGTGKTTSAAKLANY
                     YAKSGKKVLLVAADTFRAAAVAQLQTWGQRIGVEVLSASSNSDPAAIAFDGAKKAKDE
                     GFDFLIIDTAGRLHTKSNLMDELGKIQRVVEKVTLVNEILFVLDGTTGQNGIKQAETF
                     AASVNLTGIVVTKLDGSTKGGIAIATEAALQVPLKYVGFGEGIEDLTPFEADGYIAEL
                     LT"
     gene            18962..20254
                     /locus_tag="PLW53_02530"
     CDS             18962..20254
                     /locus_tag="PLW53_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRD36330.1"
                     /translation="MDEVVLSAGDTAWVLASSALVLLMTPGLAFFYGGMVRAKSALNM
                     MMMSIITIGVVSILWVFYGFKIAFGYEADSPWYGSFSTSGLGDVVNELANNGGVYPIP
                     LLAFAVFQLMFAIITPALISGAIADRTKFFSWAIFVAIWVSVVYFPVAHWVFAFGNKV
                     GDTVTGAGYLASQGVQDFAGGTAVHINAGAAGLALAIVLGKRIGWQKSPMRPHSLPLV
                     LLGASLLWFGWFGFNAGSALAANGIAALAFINTQVATAGAVIGWILTEKFRDGHPTTL
                     GAASGAVAGLVAITPACAFVEPWAAAVIGIIAGIACALAVGLKYKLGYDDSLDVVGVH
                     LVGGIVGSLAIGFFGSSKILGLDGLFYGGGVELLGKQALGVALVMGYSFVMTLIIGFL
                     IEKTIGFRVKSESELVGVDQAEHAESAYELGGVLKGGR"
     gene            20257..20595
                     /locus_tag="PLW53_02535"
     CDS             20257..20595
                     /locus_tag="PLW53_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD36331.1"
                     /translation="MKLITAIIKPGKFDDVKNALRAAGVKGMTVSEVSGVGRQGGHIE
                     VYRGAEYPIDLISKIKLEILAENKDVDSVINTIVKAASTGSIGDGKVWATPVDNVVRV
                     RTGEKGEAAI"
     gene            20698..22152
                     /gene="ffh"
                     /locus_tag="PLW53_02540"
     CDS             20698..22152
                     /gene="ffh"
                     /locus_tag="PLW53_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRD36332.1"
                     /translation="MFDSLSSKFSDLFGSLRRKGRITPEDIESTCQELRTALLDADVA
                     LSVTEGFVEKIRSRSLERLPEIRQSTNQSEEIYSIINQELIAILGGQGRRIRYAKTGP
                     TIIMLAGLQGSGKTTLAGKLARFLKEEGNTPLLVAADLQRPNAVTQLQVLAESISVPI
                     FAPEAGNGVGDPVKVARSAITYAKEKVHNVVIVDTAGRLGIDTELMQQASSIRDAINP
                     SEILFVVDAMMGQDALTTSLAFLEGVGFDGVVLTKLDGDAKAGAALSIVEVTGKPILF
                     TSSGEKLTDFDYFYPERMASRILGLGDIATLAEQAKRALDGDSAKRLEEKFVSGQDFT
                     LEDFLEQLGAMKKMGSMTKLLGMLPGANSAAMKKQIDSIDDNELVRTQAIVQSMTPSE
                     RRDPKILNGSRRARIALGSGRSVTEVNSLVDRFSAAQKAMKQMRSGGGLPAGMSLPGG
                     MSLPTASPMNSPKIAPKKKSRSGNPAKRAAQEGR"
     gene            22323..22880
                     /gene="rpsP"
                     /locus_tag="PLW53_02545"
     CDS             22323..22880
                     /gene="rpsP"
                     /locus_tag="PLW53_02545"
                     /inference="COORDINATES: protein motif:HMM:NF011093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRD36333.1"
                     /translation="MAVKIRLVRLGKIRTPHYRIVVADSRKARNGLKIEEIGRYSPAS
                     DPSLIEVNSDRAQHWLKNGAQPTPAVEAILKVTGDWQKFKGIAGQEGTLKSLPVRPNK
                     RVAYEEAVKAASNEPADGATTMKKRAAEKLAAKNAPVEVVAEAAPVEAAPAEVVAEQA
                     PIEAPVVDAPAEAVAEAAPTETPAE"
     gene            22883..23125
                     /locus_tag="PLW53_02550"
     CDS             22883..23125
                     /locus_tag="PLW53_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRD36334.1"
                     /translation="MMIDEALEHLVKGIVDNPEEVIISEKEGRRGTTLEVRVHPEDIG
                     KVIGRNGRTAKALRTVVSALAGRSVRVDLVEADEAR"
     gene            23141..23641
                     /gene="rimM"
                     /locus_tag="PLW53_02555"
     CDS             23141..23641
                     /gene="rimM"
                     /locus_tag="PLW53_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRD36335.1"
                     /translation="MLLVVGRIGRAHGVRGEATIEVRTDNPEERFAIGSILKTDPEDK
                     GPLKITGAKVHSGTLLLSFEGYSDRSAVEKLRNVLLLADVNPADSNTTEDDFHISQIL
                     GCKVIDKSGKDWGEVKEVLQLPSQDTLVLSYQGRDFLIPFVKHFTPDIDIASRIITAD
                     NLEELM"
     gene            23638..24315
                     /gene="trmD"
                     /locus_tag="PLW53_02560"
     CDS             23638..24315
                     /gene="trmD"
                     /locus_tag="PLW53_02560"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRD36336.1"
                     /translation="MKITAISIFPEYFSPLELSLIGKARSQSLIDFKSINLRDFTTDN
                     HHSVDDTPYGGGAGMVMMAEPWGQAIDSILEKDNEIDLIILTPAGSKFNQKKAEQLSG
                     AKHLVFACGRYEGIDARVGDYYRGVNGVRVHEISIGDYVVNGGEVASLVIIEAVTRLI
                     PGVIGNPASLDEESHNEIGALEYPTFTKPASWRDLNVPEVLLSGNHGEIQKWRQEEAK
                     RRSELGQ"
     gene            24453..24746
                     /locus_tag="PLW53_02565"
     CDS             24453..24746
                     /locus_tag="PLW53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HRD36337.1"
                     /translation="MATDYDTPRKTDEELHEESLEELKARRAEAQSGQVDLDEEEAEN
                     VELPGADLSGEELSVRVVPKQDNEFTCSRCFLVHHITQLARGEGAKAVCKDCA"
     gene            complement(24743..25168)
                     /locus_tag="PLW53_02570"
     CDS             complement(24743..25168)
                     /locus_tag="PLW53_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HRD36338.1"
                     /translation="MREVMRWPIWLTLLIICLDLAILLAIWASLGNQATLVTAIILTA
                     STIYFYYVTSLTLELTTERLKAGRANIEVKYLGKIEVLSKEDMLFHRGAGINPQAYLA
                     LRFWIKRGLKIEIADPRDPTPFWLISSKNPERFLERLTP"
     gene            25231..25809
                     /locus_tag="PLW53_02575"
     CDS             25231..25809
                     /locus_tag="PLW53_02575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD36339.1"
                     /translation="MSRELNIQVPPGVKIPPRHPDAPASGKLLGSHNPDCYGCGPNHP
                     AGLHLVAYAGESMSITAQFTVTDLHQGAPGLAHGGLLSCAFDEALGKLMWLVREPAVT
                     GKLETSFLAPVPVGSTLFINAEITGQDGRKIYTRAEGRIGSDSGVLAVEAAAIFIIVP
                     MEHFIKHASAEFKQVLKDRRDLSIDTEFEINP"
     gene            25806..26240
                     /gene="dut"
                     /locus_tag="PLW53_02580"
     CDS             25806..26240
                     /gene="dut"
                     /locus_tag="PLW53_02580"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRD36340.1"
                     /translation="MKVLITQLDKGLPMPSYAKAGDAGADIYSAIDITIEPGMRALVP
                     TGIAIALSDGYAAFVHPRSGLAIKYGVGLVNAPGTIDAGYRGEICVIVINHDQSESFE
                     IKRGDRIAQLVFQRVERAEFVAVEELPGSARGGAGFGSSGRS"
     gene            26237..26869
                     /locus_tag="PLW53_02585"
     CDS             26237..26869
                     /locus_tag="PLW53_02585"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HRD36341.1"
                     /translation="MSESEDKAKILSAMGGVKGLIDSGLPALLFLIAFNITKDVRDSA
                     YAALALSLLLTLVRLVKRETIQHAVSGVIGVGICAWLSMRTGKAEDFYLPGLWTNAIY
                     GSAYLVSILLRWPVIGLIIGPLLEENFRWRKDPARTKVYIKATWLWVGMFAIRLVVQY
                     PLYVSGNVNLLGTARLVMGYPLFIATAWATWIVIKSGPKLRDDLRIDKAS"
     gene            complement(26866..27525)
                     /locus_tag="PLW53_02590"
     CDS             complement(26866..27525)
                     /locus_tag="PLW53_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD36342.1"
                     /translation="MRIAIAGAGNAGRAIARELIENGHQVLLIDKNPKSLKMESVPSA
                     EWLLADACEISSLDKAKLDTCQVMVAATGDDKVNLVSSLLAKTEYGVPRVVARVNHPK
                     NEWLFDSSWGVDVAVSTPRIIAALVEEAVSVGDIVRLFSLKAGEANLVELTLPDTSSC
                     IGKSVEEISLPQDASLAAIIRDGHVITPADHDVFAAGDELIFVTTAEAEVSLKACFIK
                     D"
     gene            complement(27528..28175)
                     /locus_tag="PLW53_02595"
     CDS             complement(27528..28175)
                     /locus_tag="PLW53_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRD36343.1"
                     /translation="MGCGRVGSTLAKDFQALGHSVAVIDQDREAFRRLGADFNGLTVA
                     GMGFDRDTLLEAGIQRADAFAAVSNGDNSNILAARVARETYGVASVVARIYDPGRAEI
                     YQRLGIPTVATVLWTTDQIMRRLLPQGSRSEWQDASGKVQLAEIALHRDWYGRPVSLI
                     EQHTDARVAFVTRLGEGLIPNEHTVLQEGDVIHVMVEDKNLAATEASLALPPSGE"
     gene            28292..29446
                     /locus_tag="PLW53_02600"
     CDS             28292..29446
                     /locus_tag="PLW53_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRD36344.1"
                     /translation="MQSGDQLEVEITAIAHGGHCIARYEGRVIFVRHAIPGERVIVEI
                     SDVTKSFARGNCISVIKPSKHRVSPPCSYARFDGCGGCDFQHIDLRYQRELKSEIIKE
                     QFSRLAKMEVDVEVEEVKPNLHWRSRMEFTVSETGKVGLYASRSNQIVEIDKCLIAHE
                     DIDIAKINQMKLPKSKRVDVAITSKGKSAVVIEGRENLELIQEQVDGIEFSLNPITFW
                     QSHRMAPTVLSQVVKDYVQAKPADHIFDLYGGAGLFSAALLSDLGVAGRVTLIESDEN
                     AIIDARRNFALEPRVEIVEAKVEASLKKYRAANVVLLDPPRAGAGERVISTITSLAPR
                     TIVYVACDPASLARDSAYLSAQGYKLDQIRAFDLFPMTAHMELVARFIIS"
     gene            29505..32162
                     /gene="acnA"
                     /locus_tag="PLW53_02605"
     CDS             29505..32162
                     /gene="acnA"
                     /locus_tag="PLW53_02605"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRD36345.1"
                     /translation="MSKNSFGAKSSLEVSGKSYEIFDITKLAQANSLPFSLKVLLENL
                     LRTEDGANITAKQIEALASWNPESIPDTEIQFTPARVIMQDFTGVPCVVDLATMREAI
                     AELGGDPSRVNPLAPAELVIDHSVIADKYGTASSFKENVDIEYERNQERYRFLRWGQS
                     AFDEFKVVPPGTGIVHQVNIEYLARVVMVRAVNGAQRAYPDTVVGTDSHTTMVNGLGV
                     LGWGVGGIEAEAALLGQPVSMLIPKVLGFKLSGALAVGTTATDLVLTITEMLRKHGVV
                     GKFVEFYGPGVSALPMANRATIGNMSPEYGSTVAIFPIDQETIKYLELTGRSREQLEL
                     VEKYAKAQGLWHDPSAEPRYSERLELDLSSVVPSIAGPKRPQDRVSLSDAKSSFAKVL
                     PSYLSSNTATNPIAIEVNSRKGSITNGSVVIASITSCTNTSNPSVMIGAGLLAKKAVE
                     KGLMSKPWVKTTLAPGSKVVTNYYEKSGLTPYLEKLGFNLVGYGCVTCIGNSGPLPLE
                     VSKAVNDNDLSVAAVLSGNRNFEGRISPDVKMNYLASPPLVVAYSIAGTMDHDFDKDP
                     LGKDSSGNDVFLKDIWPSMQEIQDVIDSVVTSDMFKKDYSSVFAGDHHWTSLATPEGK
                     TFEWDPKSTYVRKPPYFESMPRNPLPVTDISGARVLAKLGDSVTTDHISPAGSIKVDS
                     PAGKYLAENGVEKSDFNSYGSRRGNHEVMIRGTFANIRLKNLLLDGVEGGFTRNFIDG
                     GKQSTIYDASMAYQDANIALVILAGKEYGSGSSRDWAAKGTALLGVKAVIAESFERIH
                     RSNLIGMGVLPLQFLDGQDANSLGLSGEEVFSISGVTALNQGRIPSIVKVTAGDKSFE
                     AKVRIDTPGEADYYRHGGIMQFVLRSLLS"
     gene            32183..34117
                     /gene="dxs"
                     /locus_tag="PLW53_02610"
     CDS             32183..34117
                     /gene="dxs"
                     /locus_tag="PLW53_02610"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRD36346.1"
                     /translation="MKHLSRIKSPGDLARFSQEELTELAAEIRAFLIEKVSKSGGHLG
                     PNLGIVELSIALHRVFESPQDVMVYDTGHQSYVHKILTGRAAGFEKLRQRGGLAGYPS
                     RAESEHDVIENSHASTALSWADGIARGFALQNQSDRTVVAVVGDGALTGGMSWEALNN
                     IAASDDLKLVIVVNDNERSYSPTIGGVATYLSTLRATKGYEKFLDWGKQVLEKTPVVG
                     NPIYETLHGMKKGIKDIVAPQGMFEDLGLKYLGPVDGHDIPALERALLQAKKFSGPVL
                     VHAITEKGRGHAPALKDEAEKFHAVGVVDPETGEPLSNNGATWTKVFSEAIVEIGRER
                     KDVVAITAAMLGPTGLDRFQAEFPERTFDVGIAEQHAVTSAAGMAYTGLHPVVAVYST
                     FLNRAFDQLLLDVGLHKAGVTFVLDRAGITGDDGPSHHGIWDLALTGIIPNAFVAAPR
                     DGARLKESLTEAVAISDAPSIIRFPKGAIGKDLPAFERRGGLDVLYRGESSDILLVSI
                     GAMAPLAIDAAAQAFREGVGVTVIDPLWVKPLPADSLLAMAPRFKRVVVLEDGIKHGG
                     IASTLSELFRENGLDLPIHSIGVPLEFIQHSKRAEILQDIGITSQSISRQLVGWASLN
                     VKQEGMQLAADENADRKPLH"
     gene            complement(34056..36155)
                     /locus_tag="PLW53_02615"
     CDS             complement(34056..36155)
                     /locus_tag="PLW53_02615"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD36347.1"
                     /translation="MAEITTPPDEVVTNALLRQVDLSAFGATGSLALITLDNGHDHTR
                     PNTFGIQSLNALNDAITAAESSDAVAIAIIGKPFIFAAGADLSGISYVAKREQSLAFG
                     KLGHDIFKRLGRSKKVTFAFINGLALGGGLEVGLHCNYRTMATTALTALPECFLGLVP
                     GWGGATLLPKLIGPERAVQVIILNALNNNTMMKAKDAQGLGVVDAVFEPADFLEKSIA
                     FAARIVSGKEKIERKDYSTDPNWDKALETGKAAIAKKYGGADIEAPRRALELISAARS
                     NTLDQGFDAEDEILANLVMGNPLRASLYAFNLIQKKRKKVEGAPKAALARKVTKAGVV
                     GAGLMASQLALLMIRNLKVPVVMTDLDQERVDKGVAWVRNEITKLVEKKRLSPEAAAR
                     LSSLISGSVSQSAFAGCDFIIEAVFEELSIKQKLFKDLEAIISAECVLATNTSSLSVE
                     KMGEGLKNPERVVGFHFFNPVAVMPLLEVARTSKTDDATTATAINVGKELKKTMIICK
                     DAPGFVVNRLLTRFMGEVTDAVDEGTPTDVADNALRPLGFPMSPFELFGLVGPGVALH
                     VSKTLNANLGPRYRISPTVSRMVEKGVKTFYVKDEKGSLSANPEALALIEKGDKPSTA
                     EQVRTRALSALAQEARMMLDEGVVATASEIDLCMLLGAGWPMHLGGILPYLDREGISE
                     AVCGQRFHPPQVASLPA"
     gene            complement(36161..37363)
                     /locus_tag="PLW53_02620"
     CDS             complement(36161..37363)
                     /locus_tag="PLW53_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRD36348.1"
                     /translation="MNTATTSTNVVLLDAVRSPFGKAGSLYAGTRADDIIVRTIRGLL
                     ERNPSVKPSEIDDVAIAAATQYGDQGMNIGRSASLLAGIPNTVPGYSIDRWCAGALTS
                     VTTLAGSIAMGAYDLAIAGGVEHMGNHPMGDGMDPNPRYLAEKLVDTDALSMGNTAER
                     LHDKFPHLTKERADKYALRSQEKTAAAYKSGKIQPNLIPVAVRNAEQGWGVATVDEPP
                     RPGTTLEALAALKTPFRPQGRVTAGNAAGLNDGATAALLASESKAKELGLSPKARMIT
                     FAFAGVEPEIMGYGPVPSTLKALDKAGLKIEDIGLFEINEAFSIQVLSFLDYFGIGDD
                     DPRVNPYGGAIAVGHPLASSGIRLILNLAEGFKEHPEVRYGITTMCIGLGMGGTIIWE
                     NPNYKKGA"
     gene            complement(37413..38234)
                     /locus_tag="PLW53_02625"
     CDS             complement(37413..38234)
                     /locus_tag="PLW53_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HRD36349.1"
                     /translation="MLSNFLIALREGLEAALIVGILVAYVVKTNRRNLLPPLWSGVGV
                     ALAISFALGGFLAFTSAELSERGEEFFAGTTSFVAVALVTWMVFWMKATARALRDTLH
                     EKVETAAMVGPLALTAAAFFAVAREGLETALFVYSNFQVAGSKTPATIGLVSGLLLAV
                     LLGYAIYRRAIKFNLSKFFTITGVALIIVAAGVLSYGIHEFQELGWLPGEESYAWDVT
                     SWMAADSIMGSLLAGTIGFDTTMSWLQLFAYGAYILGTLYLYLRPAKSLTPVKQS"
     gene            complement(38293..39597)
                     /locus_tag="PLW53_02630"
     CDS             complement(38293..39597)
                     /locus_tag="PLW53_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRD36350.1"
                     /translation="MSEQETNLRALKVPEQGTPELITTEDHLAAAIDQLKSGHGPIAI
                     DAERASGFRYSSRAYLIQIYRRGGGLHLIDPIQFNQSPLISQLNEVIRGEETIIHAST
                     QDLPCLRDWGIDPKELFDTELGARIAGCPRVGLGHLVESLLQISLAKEHSAVDWSIRP
                     LEQEWLDYAALDVALLIDLRDKVAELLEAQGKLKWAMQDFAAILKSPPPQKRKEPWRR
                     TSGMHEIKSRYQLAIIRQLWQVRDEIAASIDLAPGRLLSDAVIIALAKSNVKSKDEFM
                     KLSEAKARIRNETQKSYIETWLETFLAAQSLTQEQWPELRSRSDSLPPVKIWKAKFPI
                     AYAHISHAKAKLNEIALELNLPLENLMGPELVRKICFDQGSEQIHKMSEALLARVETQ
                     LRLNGAREWQIEKCSRSLAKSLGEGEPLPSEAPVQDDSEPQE"
     gene            complement(39604..40170)
                     /locus_tag="PLW53_02635"
     CDS             complement(39604..40170)
                     /locus_tag="PLW53_02635"
                     /inference="COORDINATES: protein motif:HMM:NF022891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HRD36351.1"
                     /translation="MKAANRNFDTFIEDIKAIKARPEISISEIPAPQKLAPYAFAITA
                     DLALDIDSEDDIATGRFVLLHDPDGQESWDGTFRCVTFVRSALDIEIQSDPMLPDVGW
                     SWFIDALKNSGASYSQPSGTVTRVASASFGELSSHDESSEIEIRASWTADNPEELMSH
                     VHAWLELMASAAGLAPLPEGVASLPQKR"
     gene            40244..41083
                     /locus_tag="PLW53_02640"
     CDS             40244..41083
                     /locus_tag="PLW53_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD36352.1"
                     /translation="MGSLLALASSLLWGTADYLAGNLSKRFKAIAVTAISQIFGLIFG
                     LVTILFFADFIRPNLSMSGYFIPGVIAGIAGFIGLIAFYTGLATGRMGVVSPISSLSV
                     LIPLFFALARGERPTSLELIGIVIAIAGAFMASGPEIRNGLPVKPLLLAVVAAIGFGT
                     ALTFIAIGSETDSLHTMTSMRVASVSICILLAIRYRSLGGIPRKEFPLLIFIGVADFL
                     ANFLLGVATTKGLVSIAMVLGSLFPIVTILLAYKFLHERLAKVQYLGIAFALGGVLLI
                     AAF"
     gene            complement(41066..41587)
                     /locus_tag="PLW53_02645"
     CDS             complement(41066..41587)
                     /locus_tag="PLW53_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36353.1"
                     /translation="MKQGPEVKTMANILLARNLATSLGGSSRELSPKSDRIRFHQIRT
                     LAKAILIGGQSYRNEPYSKLSLPLYISSRTLNEGAVGSKFIFNQDPAWLINRALLEQG
                     YPVLIEGGVNFISSLIAESLIDLLYITRVNKDGDGHFFDESNLLKNYERQSIEMIDGV
                     SFESWRVKLKSSD"
     gene            41612..41878
                     /locus_tag="PLW53_02650"
     CDS             41612..41878
                     /locus_tag="PLW53_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36354.1"
                     /translation="MMSVPLSIVAFMIIDCDSCIMRDIACKDCVVSVLISAPSNQSHE
                     PSELGLEEARVIDLLSSRGMIPPLRYAQIETESDNSQANQALSG"
     gene            41911..42927
                     /locus_tag="PLW53_02655"
     CDS             41911..42927
                     /locus_tag="PLW53_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD36355.1"
                     /translation="MKLALRWLTPYLALVLAITLLPTSSAAPSADLVAVRAEVERLQE
                     DAAEAGENAQAAKIQLARLKSQLESVRQEADLQKASVESIEKSLSAIAVARYKSTGLG
                     EGLELLFSSDPSLYLSAAGSLEDVTRKQSLELRKYATAKQRLDATSLTVSDKVRLVQA
                     AEARYRAQAAQVEAKLAQAEKLLSKLEKADREKILKLQAMDAAERQRYSKEQAKLANA
                     VSGRAGTALRYAVQQIGDNYVWGAAGPIRWDCSGLTMRSFQRAGVKLPHSSRAQFGYG
                     RSISRNNLQAGDLVFFGTPISHVGIYIGKNKMVHAPRPGAKVQIAEFGNYFGRKKYVG
                     ARRL"
     gene            42950..44101
                     /locus_tag="PLW53_02660"
     CDS             42950..44101
                     /locus_tag="PLW53_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD36356.1"
                     /translation="MPSILLLTNDFGPRAGGIETFIIGLLERMPKGEVIVYTSKQIGS
                     ESFDQKWLENFGVRVIRDSSKILLPTPRVIRAVRRVIKQSKVEKIWFGAAAPLAISAR
                     FLRVGNVKKIVALSHGHEVWWSKVFPFNFAMKEISRSVDVLTFLGDYTGSVIGKHFKD
                     KAKLVKIAPGIDVNHFKPERDPSKVNAFRSELKIGSEPLILSVGRLVHRKGQDKLITA
                     MPKILAKQPDAKLLFIGQGPRKAKLDSLVAKYGIEDSVIFLGSIAYADLPKYLNAADL
                     FVMPSRSRLMGLEVEGLGIVYLEASACALPVIAGSSGGAPDALIDGRTGYVVDGMDID
                     QIANRVLSLIDNPKVAKVMGEAGRAWVESNWSWDIWSSAFNELLEISGK"
     gene            complement(44090..44704)
                     /locus_tag="PLW53_02665"
     CDS             complement(44090..44704)
                     /locus_tag="PLW53_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD36357.1"
                     /translation="MLKVLVVDDHTMVREGLRRLIARDEEICVIGEAGSKREAFAQIS
                     HLNPDVIVVDLNLPDGNGLEVVAWARSLSQRIGIVVLTMSNMPEHVLASMQSGASSHV
                     DKASPSSELLSAIKASSVKPLSFTARKLTQAIDAKNREVGLTPRELEILEKLPTGDTV
                     LEIAAQLFVTESTVKTHLASIYKKFGAKNRVQCINTARKTGLLP"
     gene            complement(44697..45302)
                     /locus_tag="PLW53_02670"
     CDS             complement(44697..45302)
                     /locus_tag="PLW53_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36358.1"
                     /translation="MAKSKPETQESTLRLDLARRLHETLAQDLAVIGYQLDELIGDAN
                     LGKKHRIALRKIRFSISQTTKSFRDQIYQLKLTSRQQLSKQVVEILGEIKLAADFTYP
                     EFDPKVEEALSQIILELARNTSRHSKATCFYLNFKENEESIEFEIGDDGIGGVKPKDF
                     RFGLASIDELLIKISSSYSSSSKSDGSSFKFQIRKSGGNNA"
     gene            complement(45295..47082)
                     /locus_tag="PLW53_02675"
     CDS             complement(45295..47082)
                     /locus_tag="PLW53_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HRD36359.1"
                     /translation="MSEYFIPALEPKATQGNLTNLVAERAWFEPERILVSRPLGDGWQ
                     PVSAREFEAEIRATAKGLIASGIGLGDRVAIMARTRYEWTVLDFAIWFAGAVTVPIYE
                     TSSPEQVDWILSDSASVAIIVETPQLREIVTPVLNSNVKNCWVMTENVLAQLAYLGRD
                     ISDQEIDNRRNALTPDSLATLIYTSGTTGKPKGVTLTHGNFLSECGNVVMGAADLFMK
                     AGGSTLLFLPVAHVFGRMVQIGAIRAGLHLAHCPDPVGRLPIDLASFKPTFVLAVPRI
                     FEKVYNGAEAKADAAGKGAIFRKAAAIAVEYSKSLDNGGPSLTLKLKHGLFDKLVYSK
                     IRHGLGGRVEAAISGGAPLGERLGHFYRGAGVRVLEGYGLTETTAGATLNLSTAHKVG
                     SVGKPIPGTTIKIAEDGEVLIKGPIVMRGYWQNDAANKEVFTEDGYFRSGDLGKLDEE
                     GFLYIVGRKKELIVTSGGKNVAPAVLEDRLRAHPLVSQCIVVGDNQPYIAALVTIDPD
                     ALKGWITANKKDGATLAELTNDPDLIAVIQTAVDEANKAVSRAESIRKFTILPVDFTI
                     AGGHLTAKLSVKRHVVHKEFAAEIAELFG"
     gene            47136..47573
                     /locus_tag="PLW53_02680"
     CDS             47136..47573
                     /locus_tag="PLW53_02680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD36360.1"
                     /translation="MADISSHSTQIQAPIEQVREILFNLEGYPSWSSAIKTVEVLERD
                     SSSRPTKLTLKVEAGVLKDRPTLVYDWSKAPNEISFSLEEADLMTQMDGKYEIKDNGD
                     DSVTVTYSLTTALSMPVPDLMRRKVEKTTIEQSLSELKQKLEG"
     gene            47574..48518
                     /locus_tag="PLW53_02685"
     CDS             47574..48518
                     /locus_tag="PLW53_02685"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HRD36361.1"
                     /translation="MSLALGIDVGGTKVLGGVVDSSGKIVKSARKQTPPQGGQALTDT
                     IAELALELISEFDISAVGVSAAGFISSDRKTMLATPNIAGWNGVNLTEQLEKKIGRRV
                     VLENDANSAAWGEKKFGAGRDFHTVIMLTVGTGLGGGLIVNDQLYRGAYGIGAEFGHT
                     RAVPNGHLCGCGVRGCFEQYASGNALLRHAREAIAASPDLARNLLARGDSSIDGLTGK
                     HLSDAARDGDSVAIAAFNTTGQWLGALCASLVASLDPEAFIIGGGVIDAGDILLNPTR
                     QAMNELIPFTGKRPAPVIVPAVLGNDAGLVGVADLARN"
     gene            complement(48521..49498)
                     /locus_tag="PLW53_02690"
     CDS             complement(48521..49498)
                     /locus_tag="PLW53_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD36362.1"
                     /translation="MSENFRWGILGTGGIAKAFAKDLTFTSGHKVSAVGSRSLDKALD
                     FSKEYGGSAYGSYEELVASDVDAIYVATPHPMHEPNTLLALSAGKAVLCEKPFAVNAA
                     QSARMISLAKQKNLLLVEAMWSRFLPHYRLIRQLLSAGELGEIISISADHGQFLPLPK
                     HYRLHAPELAGGALLDLGVYPISLAHFILGKPNEIQVTAELTDTGVDGHCSIFFDYPS
                     GAVANLSCTMLVKTPCVAEIIGSKARIEIDSTFYNPASMRLIKTSGEVIDYPRNYEGH
                     GLREQAIEFAKLYRAGAKESDLITHQDSQEIMEIMDQIRSEIGVKYPFE"
     gene            complement(49495..50598)
                     /locus_tag="PLW53_02695"
     CDS             complement(49495..50598)
                     /locus_tag="PLW53_02695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD36363.1"
                     /translation="MSVIPNPQAFYLPSGPVAVLMIHGFNGSPASIKPWAQGLADHGF
                     SVAAPCLPGHGTTWEDMNNTTWQQWYEEVDREFTKLKQKHERVFVAGFSMGGALSLRL
                     ASIRGSEIEGLILINPAIKDTRLRVKLVPLLKYLVGSIKGSRSDVAAPNPPRHSYLRT
                     PLKAFDSLQKLWALVRQDLYLVDLPLMVGYSINDHVVDPSNSEVIIDNVSSVDIREVV
                     FERSFHNVALDYDLDILIEESRVFINDVLSGEVERSDSGSLDAQFESIVSGLSLDESS
                     PTTFLDELEQIDAIEKYPGDNKELPQLTSIQRSALLGVIGGPIYIIAVQILGLDLLGL
                     GPWPGGIALVAGIFAFFYQIKPDADEDGDGSAI"
     gene            50646..51407
                     /locus_tag="PLW53_02700"
     CDS             50646..51407
                     /locus_tag="PLW53_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD36364.1"
                     /translation="MALRRKKNKVEAMVAGDKIAYGLLKSFLLPVLTLLFRPKVSGLR
                     FVPATGPVIIASNHLSFSDSIFMPLVIPRKVTFLAKSEYFTSPGLKGLVKKLTFIALG
                     QVSVDRAGGSRSEAALLTGLSVLAEGGCIGIYPEGTRSPDGRLYRGRTGIARLAMESG
                     APIVPVAMFHTAEIQPTGKVIPKIMRVKMVFGEPMYFPTPDKSNDPEELRKATDSLMK
                     KLQELSGQEYVDIYASQAKEALDEQRRRERKESQE"
     gene            complement(51408..52082)
                     /locus_tag="PLW53_02705"
     CDS             complement(51408..52082)
                     /locus_tag="PLW53_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRD36365.1"
                     /translation="MSKAREFISPSDLTFSWNGCHRCLWLNYNHGVKAPSFFPLVGEL
                     SAMQEKHFNGAKSKDLHPEIKPGKVLDRGGWVKSMPITYNSNPSPYAIRGKYDLLMEF
                     DDGTYGVIDCKFQGKNNDKSDFYSPQLEAYAFALENPLQGEPKQISVLGLLVWSPENP
                     EGNPESGFSLNLSSAWYPITRNPVGLAERLQDFIQVISGEVPSADSTCDTCKFITTRR
                     EIFGEE"
     gene            complement(52120..52776)
                     /locus_tag="PLW53_02710"
     CDS             complement(52120..52776)
                     /locus_tag="PLW53_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD36366.1"
                     /translation="MDNSPMILDFPARYPEYLIAAMIIILAPGPSVLFIIARAIAWGR
                     ATAIASVAGNVTGAFTLSLAVAFGLGPILQASELAFIGVQLLGGLYLIYLGLDAIKHS
                     KIHADDMVNQGDIKPSNIRSIKDGFWVGALNPKGLVFFAAILPQFVDKGSEQVTEQLV
                     IMGATFAILAFFSDGTWGLIAGTVRQWLATSPQRLVLMRRIGGLVMITLGIFTLTSAI
                     "
     gene            complement(52872..54137)
                     /locus_tag="PLW53_02715"
     CDS             complement(52872..54137)
                     /locus_tag="PLW53_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRD36367.1"
                     /translation="MSLFSWKLHGNGKSIAPGEVVSTDERLSWPRTIGVGFQHIAAMF
                     GATFLVPILTGLPPTTTLFFSGVGTILFLIITQNKVPSYLGSSFAFLAPIAASVKSDS
                     MAVALGGVVATGVLLALVGLIARAVGTGWINWMLPPVVTGTIVMVIGLNLAPAAKNGL
                     ASGPVLGTLTLLAIASFAAFSRGFIGRISIFLGVVVGYLVAFVMGDVNTDGISSAKWI
                     AAPTFTTPEFKVSAIILFIPVVLVLIAENVGHVKAVSNMTGKDLDDQMGNALFADGVA
                     TTLAGAGGGSGTTTYAENIGVMAATRVYSTAAYWIAAFGAIILSVSPKFGAVLSAIPG
                     GVLGGAQVALFGMIGILGAKIWIESKVNFTDSTNLLVVATAIIIGIADVSWTSGDFTF
                     NGIINATLVAIVGYRVFHNISKSRGTSVS"
BASE COUNT        15262 a        11574 c        12920 g        14636 t
ORIGIN      
        1 tttggggtat ggggtaattg gcagcccagc tgattctggt tcagcttgtc taggttcgag
       61 tcctggtacc ccagcggaaa aaacttaata gaacttttta gttaagaaga taaatctggc
      121 tccgtcgtct agtggcccag gactctggct tctcaagcca gctacggggg ttcgaatccc
      181 ctcggagcta ccaggtatta attcttcttt gaaatatatt cactaaggcg atctgccgtt
      241 gcaactaaag ctgcagcatg gattcgacct ggctgtcttc caagtctctc aattggacca
      301 ctaatactta ctgccgcaat tactcgatta tttggaccac gcactggagc agatatcgag
      361 gccacccctg cctctctctc gccaacgctc tgtgcccatc cacgtcttcg cactgcagca
      421 agtgctgcag cattaaatct tgcgccagtt aatccgcgat gtaatttctc tgcatcttcc
      481 caggcaagaa gtatttgagc agcagatcca gcgctcatag taagagcggc gccaattgga
      541 acggaatctc gaagacctga taaacgctca gcagccgcaa cacagattct catctcacct
      601 tggcggcgat agagctgagc gctctcaccg gttgcatcgc gaagtgcaat tagcgctgga
      661 tttgctgcgg ctagtaagcg atcctcacct gctgaggctg caagctctcc cgctcttact
      721 ccaaggacaa agcgcccggt taaatcgcgt gatagcaggc gatgatgctc tagcgccact
      781 gcaaggcgat gggctgttgg gcgtgaaatt ttggtagcgg cgacaagctc gcttagcgag
      841 tggggaccag attcaagaac agagaggatc tttaccgctt tatctaagac gccaactccg
      901 ctattctttt tgtccataat ccaatattgc catctcatca tttgagaagc aagtagcaag
      961 ggagttagaa gaagttaagt atggctaaga cactggcaga aaaagtctgg gatgaccatc
     1021 tagttagaag cgctagtggg gaaccagatt tactctatat cgatcttcat ttaattcatg
     1081 aagtcactag cccgcaagcc tttgatgggc tccgccttgc aaaaagggga gttagaaggc
     1141 ctgatttaac gctagctact gaagatcata atgtgccaac tcttaatatc gataagccaa
     1201 ttgcagatcc agtttcaagg ctacaagttg ataccttgcg aaaaaattgc caagaatttg
     1261 gcatcaggct tcactcactt ggagatgttg aacaaggaat tgtccatgtc gttggcccgc
     1321 aattaggtct aactcaacct ggaatgacaa ttgtctgcgg cgattctcat acctcaaccc
     1381 atggagcctt tggcgctctt gcctttggaa ttggaactag cgaagtggaa catgttcttg
     1441 ctactcagac acttccacta agtagaccaa agacgatggc aattaatgtt gaaggaaaac
     1501 taaaacctgg agtcacaagt aaagacataa ttcttgccat tattgccaaa ataggaactg
     1561 gcggcggaca gggatacatc cttgaatatc gcggaagcgc catcagggca ctctctatgg
     1621 aggctcgaat gacaatctgc aatatgtcga ttgaagctgg cgcaagagca ggacttatcg
     1681 ctcctgatga aactaccttt gcctatctaa aagatcgccc gcatgcacca aagggtgaag
     1741 attggcagag agcgcttgcc tattggagcg aactaaaaag tgatagcgat gctaaatttg
     1801 ataaagagat tacccttgat gcagatcaac tatcgccatt tgttacttgg ggaactaatc
     1861 ctggacaggg aatacctcta gatggcgtaa ttccaagtcc gcaagatttc acagatccag
     1921 aagaggttag cgcagcagag cgcgcactgg tctatatgga ccttaaagct ggaaccccga
     1981 tgaaaaagat aaagattgat actgtttttc taggctcctg caccaatggc cgaattgaag
     2041 atctaagagc agcggcagaa attatcaagg gcaagaaagt tgcctccgat attcgaatga
     2101 tggttgttcc cggctcggct cgagtcagat tacaagcaga ggcagaaggc ctagataagc
     2161 tctttttaga agctggcgca gagtggcgaa gcgcaggatg ttcgatgtgc cttggaatga
     2221 atcctgatca attagctcct ggggagagat cagcctcaac tagtaataga aattttgagg
     2281 gaagacaagg aaagggtgga agaacccacc tagtaagccc cttagttgct gcagccacag
     2341 cgcttcgtgg aaccctctca tcaccggcag atctataacg gagaaagcaa tggaaaaatt
     2401 catttctcat accggctctg cgcttccgct gcgcagaagc aatgttgata ccgatcagat
     2461 aattcctgcc gtctatctaa agcgagttac tcgctcagga tttgaggatg gcctatttgc
     2521 tgcctggcga agtgatcctg aatttgtatt gaataaggcg gaatacagta aggccagtat
     2581 tttggtagct ggagctgact ttggcactgg atcttcaaga gagcatgcag tctgggcgct
     2641 gcaaaactat ggctttaaag tagttatctc atcaagattt gccgatatct tccgaggcaa
     2701 ctcattaaaa gcaggccttt taacagtaat tcttggccaa agtgatgttg aagctctctg
     2761 gagcgcaatc gaggcagagc catcacttga gataaaggta gatcttgaga ctcgtttagt
     2821 ttcctataaa aatcaagcaa tttcctttga tttagatgac tatacgcgtt ggcgcctaat
     2881 ggagggcctt gatgacatcg gtttaacgct tcgaaaaatt gatgaagttg agaattttga
     2941 agaaaaacgc gcgaaattca agccaaaaac gcttccggtc ttgtcataag tagcgctttt
     3001 gcgcgactct caaccttgaa ttgagagttt tttacacata aaaattgcgt tttttcacca
     3061 ttactagcaa ttaatttatt gcgacacacc gatgctattg cgcgaaaacc ttgattttct
     3121 tgcgtaaatt aatcaacagg ttaagcaaaa gcttaacttc acgcgtaaat aaggggatta
     3181 aatgaacaag acccagttca ttgcttcgtt ggctcctcac ttcaacggaa gcaagaaaga
     3241 ggcggcacac gctgttgaaa tcgtcttcga ttcaatcgtg cgcaacattc acagaggcga
     3301 agatgtcacc atcaacgact ttggtaagtt taagaaagta gatcgcaaag ctcgtaaggg
     3361 acgcaatcct ttcaccggcg aaacgatcca aattaaggca agcaagaagg ttcgtttcct
     3421 tcctgcaaag gccctcaagg agattgttgc tggagctcgt aaattagcgc cagctccaaa
     3481 gccagctcca gtagtaaagc cagtggccaa gaaggcagta aagaaggccg ctaagaaggt
     3541 tgcgaagaag aaagtcgcaa agaagaaggg caagaggaga taagtatctt ttctgataaa
     3601 ggccacgcct aagggtgtgg cctttatttt tttctatcct aggctagagc catgagcgtt
     3661 gtgcgccctg agatttctta tcttccgcct caaggagatc cgctaggggt taatccctgg
     3721 tttcgctttg gagcagcggt tttaaagcca atacttaact taatcattaa aaaggattgg
     3781 aaaggcgctc agcatctacc aatgagcggg gcagcaatag ttgtttgtaa tcatctctca
     3841 tatgttgatc cacttacctt cactcacttt ctctataaca acggtcgagc cccgagatac
     3901 cttggaaaag aatcagtctt tcgcattccc attattggca gagttataag gggagcagga
     3961 cagattccag taagtcgtga atcaaaagat gcggttaaag gtttagagca tgccattgca
     4021 gttctaaagg caggacatct tcttggcgtt tatcctgaag gaacgctaac tcgagatgaa
     4081 aatttatggc caatgaaagc taagactgga gttgctcgtc ttgcactcat tactggagct
     4141 cctgtgattc catgcgcatc atgggggcca gagaaagttt tgccgccata tagcaaaaga
     4201 ttaagactct ttcctcgaag taaagtttca attcttatgg ggcctgtagt tgatctctcc
     4261 ccctggcagg gaaagagtga tgatttagag gcagtagagc aagcagcaga tcacataatg
     4321 gatcggataa ctgaactact tgaaatatta agggggcaga aagctcctgc aattcgcttt
     4381 gatcctaaaa actcagatct tccaagaatt ggcaatttca agaaagcaaa gaaggataaa
     4441 agcaagtgaa gattgcagtt cttggctccg gtcaatgggg aagcacgcta gcccaagttc
     4501 taatcgatgc tggaaacgat gtaattatct ggggaagaaa taatgaagta gtagctgaaa
     4561 ttaattcaaa acatcaaaat cagagcgcac tcggtgagca tcttttgcct gaggcgattt
     4621 atgccacatc tgaaatcaaa gttgctttag agggagccca acttcttgtt cttgccatcc
     4681 cttcgcaatc actgaggcaa aatttggagc tctggaaagg cctaattggc cctgatattc
     4741 caattgtttc aacgcttaaa ggcattgaga tctcaactca acttcgtatg agcgaggtaa
     4801 tctgccaagt tttgggaaga gatgaatcta gtatcgccgt gctaactggg cccaatctgg
     4861 ctcgcgaagt tattttaaga cagcccgcag gagcagttgc tgcatcgact aaccagaaat
     4921 tagcagaatt aacttcaaag gcatttagcg cgccatattt tcgtgtctat acctcaaatg
     4981 atgttattgg ctgtgaatta gcaagcgcta ctaaaaacgt gattgcaatt gcagttggta
     5041 tgtcaattgg aatgggattt ggcgaaaata ctcaagcact tgttatcacc agaggattaa
     5101 atgagcttac tcgcctagct attgctagag gagcgcagcc acttaccttt gttggacttg
     5161 caggagttgg cgatctctta gccacatgtg gatcaccgct atctcgtaat cgcacattcg
     5221 gtgaagcttt aggaagaagt ggttcagtgg agcaagccca gaaagcgatc tcatcaactg
     5281 cagaggggtt aactactgcc caggctcttg ttgagcttgc tcatctagtc ggagtggaag
     5341 ccccgatcat ggaagctgtc tgcgatgtgg ttacaagtaa gattagcccg cagcaggctt
     5401 ttcttaactt aatgaagatt caaaccggag cggagattga tcacgtatga agaaaattcg
     5461 cgtggcaata gtcgcaggcg gacaaagcag cgagcatccc atctcttgta tctcggcaag
     5521 tggcgtacta gctgcaattg ataagtcaaa gtttgaacca atactaattg gcatcaccca
     5581 aagtggtact tggattgata tgcgcgaaag tgactttgta aagggaagcg atggactctc
     5641 ctatgttcca gaacagggcg agaagttagt agttgatgtt catggtatta aagatagtaa
     5701 aggcaatgac ttaggaattg atctggtctt tccacttcta cacggggcat atggcgaaga
     5761 tggaacgatt caagggttat ttgagatggc aggaattgct tatgtcggtt ctggagtttt
     5821 agcttcagct gttgctatgg ataagagttt tgctaagcca atttatgcag actttggcct
     5881 aaaagttgcc gatggaatta cgattcataa agataattgg aataagtcaa aagaccttga
     5941 gatcgcaaag attagagcgc taggttttcc aatctttatc aagcctgctc gaagtggttc
     6001 tagtcgggga acttcaaaag taaaagatga gtcggcgata agcgcagcac ttgaagatgc
     6061 tttcaattat gaccctagag ttttagttga agcagctgtg gttggtcgag agattgagtg
     6121 cgcagtgctc cagatcgatt ctgttgcaac agcatctgtt ttgggtgagg taagagttca
     6181 tccgccccat gagttttatg actttgatgc caaatacata gatagctcaa ctacctttga
     6241 tattccagca aatcttccag agtcaattgc gagcgctata agccaagcag ccattactgc
     6301 ctttgaagct cttggttgtg aagggctggc aagagtggat ttcttcttaa gtgatgataa
     6361 tcagattatt atcaatgagc taaatacaat gccaggattt actccaactt cagtttttcc
     6421 agcgctctgg cagaaaacag gcaagagcta tagccagata attacaattt tgcttgagag
     6481 cgcgttaagt agaaagaaaa acgtaattcg ctaaaaagtt ctttaaagta ctgcaaccgg
     6541 gcaggtaagt gttctagtaa ttccaggaac tgcctgaact tttgcaagaa cgcttcttcc
     6601 cagatcctcc atacttgatg cctcagctct catgattacg tcataagggc cggtgacgct
     6661 ctcggcaata gtcactccag ctatggcatt aacggcctta gttacgctgg ctgccttgcc
     6721 tacttcagtc tgaatcaaga tatatgcctg gatcgacatt gttaaccatc cttttaatga
     6781 agatattagc ctgagtcgcc ccgttggact gcccactctt tatctcacac ttgttatgtg
     6841 gtgagaggtt agcgcattta ggctagcctg cgtaagtatt aattcttaag ggagtaggcc
     6901 ttgcgcgaag atgaagtact aaagaatgtg cgcgccatct ttgattatgg ccagagcgat
     6961 tcagctctga tcgttggaaa tggcgatgat ggcgcagttc ttggcccgcg aagcgaactc
     7021 accgttcttg ctagcgatat ggcagttgag ggaattcact ttaactttgc ctggagtagc
     7081 gctatgcaag ttggccgaaa aattagcgct gcaaacttgg cagatatctg cgctatgggc
     7141 ggctggccag aatacttctt agtggcttta gcatttcctc caagtttctc aggccaggtg
     7201 cttgagttag ctaagggcat taaaaccgag tgccaaagag tgggcgttaa agtaattggc
     7261 ggagacttaa gtagatcaaa tgaagttgta atctccatta gcgcaattgg aagagttaaa
     7321 agaccaatac ttcgaagtgg ggcaaaagtt ggagaaagac tctttatttc aaatcttcca
     7381 ggattttcgc aagccggtct ctcgctgctt aagagctcta gtgataatca gagccctgca
     7441 gcaaataggg ctcgcatggc tcatctagca cctgatttag attacgagaa atatcgaaag
     7501 agttatgagt acttatcatc tgcaatagat actagtgatg gacttgtcat tgatgcatct
     7561 cgcattgcgg cagcaagcgg agttggaata gatataagag ttgaagcgct aattaatgct
     7621 ccagaattta gcgaacttga aagtgcgagc ggatctagag agagcgccct tgaaagcgct
     7681 ttatatgggg gagaggatca cttacttctt gccactagcg atgctcaaga ttttggattt
     7741 cttgaaatcg ggcaggtaat aaaaggtgat ggaatttatc tagatggagt taaaactaag
     7801 acttccggtg gataccaaca tagttggtag gccaaatcaa ttagcggaga acttttccag
     7861 ccttgaggca ggaggtgcag acgttcttgc gcttggaata accgtttatg agagtgcgaa
     7921 ctctttgaat atttggattc caacgacggc gagtacggcg ctttgagtga gagacattgt
     7981 taccaaagct tggtcccttg ccacagatat cgcatacagc tgccacgtta ctactccttg
     8041 aatttgccct tgattgagct tggttagttg cgggcgcaag attagccgca aaggcgctac
     8101 tttcgccaat taccgcccaa tctatgcgag aaatttcccg acttggcgag cagagcgcac
     8161 ctaaggctta tcaccgccac ctgagagaat gaagcatggc aagtctggat tcatcactgc
     8221 tatcaatact tggtgataga acagccgagg tattggatga gaatttaggt cttaaaacta
     8281 tctcagatct cctgcgtcac tatcccagac gatatgtcat tcgcggcgaa cttaccgata
     8341 ttgaaagctt gattgaaggc gaggaggtaa caatctttgc caaggttgaa agtagcaaag
     8401 tcaaaagaat tccaggtagg aagagcgcaa ttcttgaaac tgtcgtaact gatggacgag
     8461 caaaattaac gttaactttc tttaatcaag cttggcgcga gaaagaattg cgcccaggaa
     8521 gacagggttt gtttgctgga aaagttggat tgtttaaagg aaaacgtcag ctagctcatc
     8581 cagattactt acttattcct gaaggcgatg atgtggacca ggcaattggt gattttgctg
     8641 gaaagtttct tccggtctat ccagcaacag caaagatgcc ctcatggaaa attgccaagt
     8701 gcgttcgctt agcacttgat ggacttgatg aaatcgcaga gtatttaccg caagaattac
     8761 tatttgaatt taattatcca aagatttccg aggcctttag gcaggtccat cagccagata
     8821 gcgctgagag cgcagagttg gcgcggcagc gcctgacctt tgatgaggcg cttttaatgc
     8881 agttattttt agtcttgcga agatatgaag taagagcggc aaagacaaca tcacgcgcac
     8941 caagaaacga tggagtattg gctgcttttg ataagcgact tccctttgaa ctaacagcag
     9001 gacagaggca agtatggagc gagatagtta aggatctctc tagcgatcac ccgatgtatc
     9061 gtctcttgca aggagatgta ggttctggaa aaactatcat tgcgctacgt gcaatgctcg
     9121 cagtagttga ttctggcgga caggcggctc ttctcgcgcc cactgaagta ttagcccagc
     9181 agcactatcg aaacttttta tcactactta aagatttagc tgaggcagga atgataggcg
     9241 ggaatcaaag cggcctacag gttcgtcttc taacaggatc cactccagct gcgcagcgac
     9301 gcgaagtgct ttcagcaatt tcatctggtc aagcaggaat aacaatagga acccatgcac
     9361 tattgggcga gagtgtaaat ttcaaagatc ttggacttat tgtcattgat gagcaacatc
     9421 gatttggtgt cgagcagcga gatgcgcttc gagataaggc tagagaacca gctcacgtat
     9481 tagttatgac agcaactcca ataccaagaa ctgtggctat gacaatcttt ggagatttag
     9541 atgtatcaac tcttagagac atgccacttg gtagatctcc gatcacctct cacgttgttt
     9601 cacaatccag caaaccagag catctaactc gagcatggca gagagcttgc gaggaagttg
     9661 gtaattcaaa tcaggtctat gtagttgctc ctagaatttc agcagatgat gaatcagatc
     9721 tgcagagatt tgggcttacg ccagatgaat taattcttgc aaagaatcta atagatgatg
     9781 aggatagctc ctcgctaaag cttgcaatga gctcagttga agagctttat cccatgctaa
     9841 cagctgatgt aatgaaagat ttaagagtgg gaaagctcca tggtcgccta tcttccgagg
     9901 agaaggaggc ggtaatgcaa gcctttgtac gacatgagtt agatgtttta gttactacaa
     9961 ctgtgattga agttggcgtt gatgttgcaa atgccacgat gatgataatc atgaatgcag
    10021 aaagatttgg tgtttcacaa cttcatcagc tccgtggtcg tattggccgc ggtgtttctc
    10081 ctggtctctg tttatttgtc acttctgcta gcgaggattc acctgcaatg gccaggttaa
    10141 aggcagttgc tgcaacaact gatggctttg aactcagtcg ccttgattta caagagcgta
    10201 gcgagggtga tgtcttggga agcgctcaat ctggagcaag atcacactta aggcttctaa
    10261 gagttattag ggatgaggaa ttaattgctc aagcgcgcct ggtagcggag aatctgctca
    10321 gtcaagatcc aacgctttct gcaaacccag aacttgctca agcagttgaa aagttaagac
    10381 aagaagaacg agctgcctat ttggagaaac gctaaatgag aattattgcc ggccttgcaa
    10441 agggaagaaa tataaattca ccaatttccc aagttcgtcc aacctctgat cgtgcaaggg
    10501 aggccctctt ctctactctt gaatctgaat ttggttctct tagtgatgca aactttcttg
    10561 acctctactg cggttcagga gctgtttctt gtgaagcgct ctctcgtggc gctggggtgg
    10621 tagttgcagt tgataatgaa gagaaagcaa cttcggtaac acgtagtaat tttgaattgc
    10681 ttgaaaaaat tcctggcatt ggttccgcct cagtcataac aatgtctgct ggaaagtatt
    10741 tggataaagg cgctgatttg gcttttgata ttgttttcat tgatccaccg tatgacttgc
    10801 caaatagtga tgttgaaaaa atattgttat cacttgtcaa taatggattt ctaaagagtt
    10861 cctcaataat tgcagtagag cgcgattcaa aaaccaaacc cttttcatgg ccccagggct
    10921 tagctgagtt gaaggttcga aagtatggcg cagccagtat ttactacggc gaacctaggc
    10981 aatagcgtat ttagtaattc gtgttagcgt tgccccgtga agaaagcagt atgcccgggc
    11041 tcctttgacc caatcactaa tggacacctt gatgtaattg aacgtgctag tcatttattt
    11101 gacgaggttg taattgcagt tcttgtgaat aactcaaaga gctctctgtt tacgatagag
    11161 gagcggatag ttctagctag agattgcgtg aagcatttgc cgaatgtaaa ggttgatatg
    11221 tggagtggtc tattagttga ttactgtcga gagaataaag ttgatgcaat tgttaaaggg
    11281 ctgcgcgcag tgagtgattt tgactatgaa cttcagatgg cccaaatgaa tctgcaatta
    11341 aagggcgttg acactcttct tatggcaact aaacctgcct attccttcct ttcatcatct
    11401 ctagttagag agatcgcccg ctatggcggg gatgtttcaa atctggttcc tgctggagtt
    11461 ttaagtgagt taactaggaa agcaaaagat tctccttcgc caagttcgca atcaggaagg
    11521 tcataacatg gaagcaatag agaaactcca atctgccata actatggtta atgaggcacg
    11581 ctcagttcca ttatcagctt cttgtgtagt tcatcgaggt gagatgttgg aactgcttga
    11641 agagattaat caagcactac caaaagatct ctcaaatgcg cagaagctcc tcggaagcag
    11701 agagacaata attgaagagg gtagacagag cgctgagcag ttaattgctc atgcaagaga
    11761 agaagttgct cgaatggttg agcagactgc aattgttgca gccgcaaggg aggagtctgc
    11821 aagattaatt gatgaggctc actcaaaagt tgaaaaagaa cgtgaagaag ttgatgccta
    11881 tatcgattct cgtctagcaa ctcttgaagt aattttaaat aagacgctag aggcagttaa
    11941 tcgagggcga gaaagattgg ctggggcaag tgataaagat gtactctctc aactagcaga
    12001 gtagtaatta cgtgaactcc aagactgaat caccatttaa agttaatttc cacgaacttc
    12061 caaggcgagc tggggagttt cgccaatatc acctgcaatt tagcgcacca caggcaatcg
    12121 gtatcgctct acttgagatt ccaaagtcag agccgattga tatctcattc aaggctgaat
    12181 ctgtcgatgg cggagttcta ctgaccggaa ctgttatctc tcatgccaag ggcgaatgcg
    12241 gacggtgttt agacccaatt gactttgacg ttaatcaaaa attccaagag ctatttctct
    12301 attcttcaaa agttagtgag gtcgaagacg atgaagatga ggtctttagc cttgatggtg
    12361 atattgcaga ccttgaagtt ccgattcgcg acgccgttat tctttcgatg ccaatcaacc
    12421 ctctctgcca gagtgattgc ttgggactct gctcagtttg tggcctgaag cggcgggagc
    12481 tggaaaagga tcacgcccac aaggccagtg atccgagatg gagcggattg gctgactgga
    12541 agccctaatc aggcgtgatt ttgcaaagat atcgaagctg agcaatactt agccctcaag
    12601 tttgaagtag taatttttaa aaggagaata acgtgccatt accaaagcga aagatgtcgc
    12661 gctcgcgcac tcgttctcgt cgtagccagt ggaaagcaac acctgcatcg actgcaaatt
    12721 gcccacaatg tgcgcaacct aagttgcaac acactgcttg ccctacctgc ggcacctata
    12781 accgtcgtca agttctagag gtttaatctg agtctggctc tgaccgaaaa gcttggagtt
    12841 tcggtaaaac cagagttact cgaactcgcc ttaactcacc gctcctttgc ttatgaattc
    12901 ggtggtcttc caacaaatga acgcttagag tttctaggcg atagcgtcct tggtttagta
    12961 attaccgaag cgctctatga acgctttccg gatcttgatg aatcacgtct ctcaccactt
    13021 cgctccggga tagtaaatat gcgcgccctt gcaagtattg caagagagct agaactcggc
    13081 tcagcgctaa aaatcggcaa aggtgaagag gtaactggag gaagagataa gaattcaata
    13141 ttggcagatg cctttgaggc tttggtggga gcgatctatc tagatcatgg ttttgatgtt
    13201 agcgcagaga tcattatgcg tctgatgaaa agcgcgattg atgaagcagt aacaagggga
    13261 gcaggtcttg atggaaagac tgcgctgcaa gaaattgttg cctcctctgg atgggcgcca
    13321 cctgaatata aagtcagtga gtcaggccca gatcacgata aagattttgt tgcctatgca
    13381 attgttaatg gagttacata cccgcaaggt catggcaaat ccaagcgcga agccgaacaa
    13441 gttgcggcaa gaattgcctt tgaagctctt tcaaataact aacttatgcc cgaattaccc
    13501 gaagttgaaa cggtaagacg aggtttagag ccattcattg ttggaagaag aattaaagaa
    13561 attgatgtaa tccataaagg tggaaataga tcaacaactt atgctccgta tagcgctatt
    13621 gaaggcgcaa aagttttgag tgttaatcgg cgtggaaagt tcctatggtt tgaattaaat
    13681 cgagattttg cactaatggc ccacttagga atgagcggac agctattgat tcaagataag
    13741 cagagagaat atgagaaaca tctgcgagtt cgcattgatt gcggcgatag aagaaaagag
    13801 atccgatttg tcgatcaacg aacctttggt tggatttcaa ttgataagtt ggtcttagaa
    13861 ggccaagacc taatccctca atcatttact cgaattgcgc ctgatttgtt tgataagaaa
    13921 tttgatcgca gcgccgtgat cggccggatt agtaaaaaga atagcgcgat aaagcgagta
    13981 cttcttgatc aggccgtggt aagcggtgtg ggtaatatct acgccgatga agcactctgg
    14041 cgcgccaaga ttcatcccga gcgccttgca aagaaactat ctgagcaaga aatttctaat
    14101 cttcttgatg ccactaaatt tgtcatggag aaagccttga aggcaggcgg aacaagtttt
    14161 gatgagctct atatcaatgt aaatggcgag agtggctact ttgatgttga gcttgaggca
    14221 tatggccagg agaatgagcc ctgttctaga tgtggctgcg agattcgcag gattgctttc
    14281 gcaaaccgct cttcacattt ttgtccgcaa tgccagagca aatctccctt gaagaaagcg
    14341 ggaaaaaaga aggttagaaa aaaagtcagc tctaagcggg ggtaaaaccc gagcgtgcgc
    14401 gggctcaccc gataggttct acccgtgtat ttaaagagca tgaccctgaa gggctttaag
    14461 tcctttgccg ctccaacgac cttgaagttc gagcctggga tcacctgcgt ggtgggacca
    14521 aatggttcgg gcaagagcaa tgtcgttgat gctctctctt gggtaatggg cgagcaggga
    14581 gcaaagagcc tgcgcggtgg aaagatggaa gatgtcatct ttgctggaac ttcaggaaga
    14641 gcgcctctgg gacgagcaga agtatcagtc actatcgata acgcagatgg cgcactacca
    14701 attgaattta gcgaagttac tatttcaaga actttatttc gaaatggtca gagtgattat
    14761 caaatcaatg gtgaatcagc tcgccttcta gatattcaag agctattaag tgatagtggc
    14821 atcggacgcg agatgcacgt catcgttggt cagggacaac ttgatgccat cctcacagca
    14881 aatcctgagg agcgcagagg atttattgag gaagctgctg gaatattaaa acataggaag
    14941 cgaaaagaga aagcacttcg aaaattagat tcgatgcaag gcaatatgtc tagagtgcaa
    15001 gatctcacca atgaattaag aaggcaactt cgcccgcttg gtaaacaggc agaggttgct
    15061 aggaaagctg ctgtgattca ggctgatgtg cgagatgcga agctaagaat tctggctgat
    15121 gacttaattg cattcaattc aacactaagt ttagaagttg ctgatgaaac tgctcttcgt
    15181 cagagacgca gtgaagttga tcaagagctg gagcaattac gtaaaagaga gattgagcta
    15241 gatcagatag cagcaattca gggaccagag ttagccagtg cgcagaataa ttactacagc
    15301 ctaactgccc agaaagaaaa gctaagagga actcagaact tagcatcgga gcgtgcgcgc
    15361 ttcttagctg aagaggcaga ggaagctaga gcatctgggc gagatcccga agcgctagat
    15421 gcggaagcaa aagttttacg aggcgaggaa tctgttctgc aaggttcatc agataaagca
    15481 agagctgaat tagttgccgc aagtaatagc gttactcaat tagaagcgcg gttaaagggt
    15541 gaagaagatc gagtggccgc agcacagcga gcgcttgctg attatcgaga aggaactgcc
    15601 agacaagaag ggcatataaa gtctctgcaa tctcgaatcg atggcgcggg ctctgaaatt
    15661 aataggttag agagcgcgtt aactggagcc aaggcaagag tggctgaagc taaagttgaa
    15721 tttgctcgac ttgagagtga aattgccggc cttgatgcag gagagctcgg ccttgatgca
    15781 gaatttgaaa aagcaaaagc tctgcttaat ggcagtaaag ctggccttga aaagttaaga
    15841 agcgatctct ctttagcgga gcgagcaaga gctgcttcgg aggcaaaaat tgatgcgctc
    15901 cggcaagcga cgcttcataa ggatggaagt ggtgcgctat taacaaatga tcaggggata
    15961 gtaattcgtg gcagcctagc atcactttta acagttgagc ctggctggca gagcgcagtg
    16021 accaatgcgt tgggctcatt agcaaatgcc ttagtgcttc aagatctaaa tcaggttatc
    16081 tctgcaatca ctttcttgaa atcaaaatct gctggaagtg ctgagctgtt atttatatcc
    16141 gaaggtgatt caaggccagt tgttgagatt ccaattggat tgacaccact agcaagtaaa
    16201 gtttcatcag ctactttaaa ttccattttg ccggctcttt tctctggcct ggttgctgca
    16261 gaatcactta gtcaagcaag tcagatcatc gccagcaatc catcacttat tgcagttact
    16321 cgtgatggtg atttggttgg aagaggaaga gcaagtggcg gctcatctgc tcgcacctca
    16381 ttaattgaga tttctgaaat gattgaaaaa gctgagattg agctacagcg acatggccat
    16441 gattgcgatc gaattcactt tgatatttct actgctgaaa caaaattaag atctgatcaa
    16501 ctcacctacg atcaagcctt agctagatta aatgagtcag atgctgctat ggcagcactt
    16561 gctgagcaga tggccgcagc tagccaaggc gtaaagagcg ctagcggcga ggttgagcga
    16621 ctagaaaaat ccattacgga attaaagcaa gcgctaaata atgatttaag cgagctaaat
    16681 attgctagaa gtgaatttga gaaaagtagt gaaccgttgg aacctgagtt aagcactctt
    16741 gaaaatctaa gagcaactct tgctacagca agagcagttg aagttgaagc tcgtcttgcg
    16801 cttcgaacta ttgaggagcg agtagcagcc atctcgcagc gggctagaga tcttgaactt
    16861 gcagcatcaa atgagcgcca agcggcaagt aataacatcg ctcgccgcgc agctagagca
    16921 aggggcgcag ttacggcgca agctattgcg gatgctgcct atgaagcctt aattcaaatt
    16981 gagagctcta tctccagggc aaatagtgag cgggaaagac ttgaactggc tcgctctaat
    17041 cgagatggcg aaattctctc tattcgccaa gcaatccgag atctaagcgt tgaacttgat
    17101 caattaactt ccagtgttca tcgcgacgag atagctagag ctgaacaaag attgagaatt
    17161 gaacaacttg aaataaaagc tatggaggaa tttggagttg atagttcatc tcttgtaaat
    17221 gagtacggtc cagataagcc agttccaacc ttcatcgaag atgacgctgg aaatttgagt
    17281 caaggcgaat tcattccgta tgtccgtcaa gagcaggaaa aacgtctagc tggagctgag
    17341 aaatctctgg ctcttcttgg caagatcaat cctttagctc ttgaggagtt ctcatccctt
    17401 gaagagcggt tgaaatatct ggcagatcag ttagaggatt taaagaaatc aaagaaggat
    17461 cttctagaca tcatcaaatc agttgatgac aaggtccaag agatttttga agaagctttt
    17521 agagatacgg cgcgagagtt tgaaaaaatc tttgctcgac tctttccagg gggagagggg
    17581 cgtctgctct taactgatcc aagtgatctt cttaattctg gcgttgatgt tgaagctcgc
    17641 ccgccaggaa agagaattaa gcgactatca cttctttcag gcggtgagcg ctcattgact
    17701 gcggttgcgc ttttagtggc aatttttaaa gcgaggccaa gtccgttcta tgttttagat
    17761 gaggttgaag ctgccctaga tgacacaaat cttggtcgcc tactctcagt tctagaagag
    17821 ttaaggcaag catcgcaatt aattattatc acccatcaaa aaagaactat ggaaattggc
    17881 gataccttgt atggcgtaag tatgcgcggt gatggagtat cagaagttat ctcacaacgt
    17941 cttcgcgaga ttgaaactga acccgcttaa ccatgggttt atttggctct ttcctcagca
    18001 agttctcaaa atctaaagca acaattgaag atcttgaaca gtttaggcag attctaatct
    18061 catcagatat tggggtaggg ataactgaag agattttaga tttagtaaag cgcgagaaga
    18121 gcgaagagct ctcaacagct atcgctagct cacttaaatc aaagttaact acctcatcaa
    18181 gagttataaa tttatcaagt gatggaccaa ctgtaattct aattgttgga gttaatggca
    18241 ctggaaaaac cactagcgca gccaagctag ccaattatta tgcaaagagc ggaaagaaag
    18301 ttttattggt tgctgctgac acctttaggg ctgctgccgt tgctcaactt cagacttggg
    18361 gccagagaat tggcgttgaa gttttaagcg cttcaagcaa tagcgatccg gcagcgatag
    18421 cttttgatgg ggcaaaaaaa gcgaaggatg aaggttttga ttttctgata attgatacag
    18481 ccggaagact tcatactaag agcaacttga tggatgagct aggtaaaatt caaagagttg
    18541 tagagaaggt aactttagta aatgagattt tatttgtact tgatggaaca actggtcaga
    18601 atggaatcaa acaggcagag acctttgctg ctagcgtgaa tttaactggg atagttgtca
    18661 caaagcttga tggctccacc aagggcggta ttgcaatagc tactgaggcc gctctgcaag
    18721 ttcccttgaa gtatgttgga tttggtgagg ggatagagga tctgaccccc tttgaggctg
    18781 atgggtatat cgctgaactt ttaacatagc cgtaacagag ggtgaagaag tgtcaccttg
    18841 gtgaaatcta tttgagccat caggggaaat acttctaaag catgatctgc tcattctcag
    18901 agttgagata tttacctttt attcacggag tctgcattta gcagcccggt tcggagaaaa
    18961 aatggatgaa gtagtactaa gtgctggcga taccgcttgg gttttagcta gctctgccct
    19021 ggttttacta atgaccccag ggcttgcttt cttctatggc ggcatggtgc gagcaaagag
    19081 cgcgctgaat atgatgatga tgtcgataat cactatagga gtagtaagta ttctctgggt
    19141 tttctacggt tttaaaattg catttggcta tgaagctgat tcaccctggt atggatcttt
    19201 ctctacatct ggattaggtg atgtagtaaa tgaattggca aataatggtg gcgtctatcc
    19261 aatcccgctt ctagcatttg cagtattcca acttatgttt gccattatta ctccggcgct
    19321 gatctctggc gcaattgcag atcgtacaaa attcttctca tgggcgatct tcgttgctat
    19381 atgggtatca gtggtttatt tcccagtagc tcactgggta tttgcctttg gaaataaagt
    19441 tggcgataca gtaactggcg cgggatatct tgcctcacaa ggagttcaag acttcgcagg
    19501 tggaactgca gttcatatca atgctggcgc tgcaggtctt gcactagcaa ttgttcttgg
    19561 caagagaatt ggttggcaga aatctccaat gcgtcctcac tcacttccac tagtgctact
    19621 tggcgctagc ttgctctggt ttggatggtt tggatttaat gctggaagcg cacttgctgc
    19681 taatgggatt gcagctctgg catttatcaa cactcaggta gcaacagcgg gagcagtaat
    19741 tggctggatt cttacggaga aattccgcga tggacatcca acaacacttg gcgctgcatc
    19801 tggagctgtt gctggccttg ttgctatcac tcctgcttgc gcattcgttg aaccatgggc
    19861 agcggcagta attggaatca ttgctggaat tgcctgcgca cttgcagttg gtcttaagta
    19921 caagctgggc tatgacgatt ctctagatgt tgttggcgtt cacttagtag gaggcattgt
    19981 tggcagtttg gcaattggct tctttggaag tagcaagatt cttggtctag atggactctt
    20041 ctatggtggg ggagttgagc tccttggcaa acaagctctc ggtgttgctc tagttatggg
    20101 ttactcattt gtaatgacgc taatcatcgg attcctaatt gaaaaaacaa ttgggttcag
    20161 agtcaagagc gagagtgaac tagtaggagt cgatcaggca gaacatgcag agagcgccta
    20221 tgaattaggt ggagttctca agggaggtcg ttaatcatga agctaattac cgcaatcatt
    20281 aagcccggaa agtttgatga tgttaaaaat gccctaaggg cagctggagt caaaggaatg
    20341 acagtctctg aagtatctgg agttggtcgc caaggtggcc acatcgaggt atatcgcgga
    20401 gccgaatatc ccatcgactt aattagcaaa ataaagttag agattcttgc tgagaataag
    20461 gatgttgatt ccgtgatcaa caccattgtg aaggcggcat ctacagggtc aattggcgat
    20521 ggcaaggtct gggccacccc tgttgataat gtagttcgcg tccgtaccgg cgagaaaggt
    20581 gaggctgcaa tctaataatt gcccgcatct aggcagtgat acttgaaaaa aggtggaata
    20641 tgcgagggcg tatccacctt ttttctattt gcctagccat tatgtaggct taaagacatg
    20701 ttcgattcct taagttctaa gttctctgat ctctttggat cacttcgtag aaaagggcga
    20761 attacccctg aagatattga gagtacttgc caggaacttc gcaccgcctt acttgatgct
    20821 gatgttgctt taagcgttac tgaaggcttt gtagaaaaaa ttagaagcag atcacttgaa
    20881 agacttccag agattaggca gtcaactaat caatcagaag agatttattc gattatcaac
    20941 caggagttaa ttgcaattct tggcggacaa ggacgacgaa tccgatatgc caaaacaggt
    21001 ccaacgataa taatgcttgc aggtcttcaa ggatctggaa aaacaacctt ggcgggaaaa
    21061 ttagctagat ttctaaaaga ggagggaaat actccgcttc tagtggctgc agatttgcag
    21121 agacctaatg ctgttacgca attacaagta ttagccgaat caatttcagt tcctatcttt
    21181 gcgccagagg caggaaatgg agttggcgat ccagtaaagg ttgccagaag cgcaattacc
    21241 tatgccaaag aaaaagtcca taatgttgta attgttgata ctgcgggaag acttggaatt
    21301 gatactgagt taatgcaaca ggcaagctca ataagggatg caattaatcc gagtgaaatt
    21361 ctctttgtgg tagatgcaat gatgggtcaa gatgccctta ctacctcgct agccttttta
    21421 gagggagtgg gttttgatgg cgttgtatta acaaaactag atggcgatgc aaaagctgga
    21481 gctgctttat ccattgttga agtaacagga aagccaattc tctttacctc aagtggggag
    21541 aagctaaccg actttgatta cttctatccc gagcgaatgg cttccagaat tttgggcctt
    21601 ggcgatatcg caactttagc tgagcaggca aagcgagcac ttgatggcga tagcgctaag
    21661 cgtctggaag agaaatttgt ttcagggcaa gatttcacgc tagaggattt cttagagcaa
    21721 ttaggggcaa tgaagaagat gggttcgatg accaaacttc ttggcatgct gcctggtgct
    21781 aactctgctg caatgaaaaa gcagatagat agcattgatg ataacgagct agttagaaca
    21841 caggctattg tgcaatcgat gacgccaagt gagaggcgag atccaaaaat attaaacggt
    21901 tcaagacgag cacgtattgc actaggtagc ggcagaagcg ttactgaggt taactcttta
    21961 gttgatcgat tctctgctgc gcagaaagcg atgaagcaga tgagatcagg tggcggatta
    22021 cctgcaggga tgtcccttcc tggaggaatg agccttccaa ctgcttctcc aatgaattcg
    22081 ccaaagatcg cgccgaagaa gaagtcacga tctggaaatc cagccaaacg agcagcccaa
    22141 gaggggcgat agctaagctc aattgggaaa tatcaagggc tcgtggcaat attggcctcc
    22201 tgttcaaggg ccctctacct cttgaccggc acttataaac gtaagaactg tttttcttca
    22261 cttggcatcc tactcaagga agaaaagata agttcgatta actatgcaag gagaaagcct
    22321 tcgtggccgt aaaaattaga ttggtacgtt tgggaaagat ccgcactcct cactaccgaa
    22381 ttgttgttgc agattcccgt aaagctcgta atggattaaa gattgaagag attggtcgtt
    22441 actcaccagc atctgatcct tcgctaattg aagtaaattc agatcgcgct caacattggc
    22501 taaaaaatgg tgcgcaacca actccagcgg tagaggcaat tttgaaggtt actggtgatt
    22561 ggcagaagtt caagggcatt gctggtcaag aaggaactct taaaagcctt ccagttcgtc
    22621 caaataagcg cgttgcttat gaagaggcag taaaggcagc aagcaatgag ccagcagatg
    22681 gcgcaactac tatgaagaag cgtgctgctg agaaacttgc agcaaagaac gctccggtag
    22741 aagttgtagc tgaagcagca cctgttgagg cagctccagc agaagttgta gctgagcagg
    22801 ccccaattga agccccagta gtagatgctc ctgcagaagc agttgcagag gctgcgccaa
    22861 ctgaaactcc agctgagtaa atatgatgat tgatgaagct ctagaacatt tagttaaggg
    22921 aatcgtagat aaccctgaag aggtaataat ctctgaaaaa gagggaagac gcggaaccac
    22981 ccttgaggta agagttcacc ctgaagacat tggcaaggta atcggtcgca atggtcgaac
    23041 agcaaaggcg ctacgcactg tggtttcagc tcttgctggg cgaagtgttc gcgtagatct
    23101 tgttgaggcg gatgaggcgc gttaatcgcg cttagctaat ttgttacttg tagtaggtcg
    23161 catcggtcgc gcacatggag tgcgcggcga ggcaactatt gaagttcgaa ccgataaccc
    23221 tgaagagaga tttgctattg gttcaattct aaaaacagat ccagaagata aaggtccctt
    23281 aaagataacg ggcgccaaag tgcatagcgg aactctgctg ctctcatttg aaggctatag
    23341 cgatagaagc gcagttgaaa aacttcgaaa cgttctgctt cttgctgatg ttaatccggc
    23401 agattcaaat accactgaag atgattttca catctcccaa attctaggtt gcaaagtaat
    23461 agataaatct ggcaaagatt ggggcgaggt caaagaggtt ctccagcttc catctcaaga
    23521 cactctggtg cttagttatc aggggcgaga ctttttgatt ccatttgtta agcacttcac
    23581 cccagatatt gatatcgcaa gtaggataat tactgccgat aatctagagg agctaatgtg
    23641 aagataactg ctatctctat cttccccgaa tacttctccc cccttgagct ctccctaatt
    23701 ggtaaagcta gaagccaatc attaattgac tttaaaagta ttaatttaag agatttcact
    23761 actgataatc accatagcgt tgatgacact ccttatggcg gtggagccgg aatggtgatg
    23821 atggcagagc catggggcca agccattgat tcaatattgg aaaaagataa cgaaattgat
    23881 ctgattatcc tcaccccagc aggctctaag ttcaatcaaa aaaaggctga gcagttaagt
    23941 ggggctaagc atctggtctt tgcctgtggt cgctatgaag gaattgatgc aagagtgggc
    24001 gattactatc gcggagtaaa tggagtaagg gttcatgaaa tatcaattgg tgattatgta
    24061 gtaaatggcg gcgaggtagc ctcgctagta ataattgagg cagttactcg tttaattcct
    24121 ggagttattg gcaatcctgc atcacttgat gaggaatcac ataatgaaat tggcgcactg
    24181 gaatatccaa catttactaa acctgctagt tggcgagatc taaacgttcc tgaggttttg
    24241 ctctctggaa atcatggcga aattcaaaag tggcgacaag aagaagccaa acgccgctca
    24301 gaattaggcc aataggctta acaaacgggg aataatcgcg acacgccgag tgttggatag
    24361 gacagcgagc aagtagttgg gcattatccg cccgtagcca aatgactaaa tggtcaggct
    24421 ctatgtagga agagattggt ggtgagagat caatggcaac tgattacgac acgcctcgaa
    24481 aaaccgatga agagttacat gaggaatccc tagaagagtt aaaggctaga agggctgaag
    24541 ctcaatcagg tcaggttgat ctcgatgaag aagaggcaga aaatgttgaa ctgcctggag
    24601 cagatttatc tggtgaagag ttatcagttc gggtagttcc aaagcaagat aatgaattta
    24661 cctgttcacg ttgcttttta gttcatcaca ttactcaact agcgcgcggt gagggagcta
    24721 aggcagtctg taaggattgc gcttaaggag ttaatctctc taagaatctc tctggatttt
    24781 tagaacttat caaccaaaaa ggtgtgggat cgcgcgggtc tgcaatctca atttttagtc
    24841 ctcttttaat ccagaagcgc agcgcaagat aagcctgcgg attaatccca gctccacggt
    24901 gaaacaacat atcttctttg cttaaaactt ctatcttccc taggtattta acctcaatgt
    24961 tggccctacc agccttaagt cgctctgtag tgagctcaag ggttagtgat gttacatagt
    25021 aaaaatagat agttgatgcg gtaagaataa ttgcagtaac tagtgtggct tgattaccaa
    25081 gtgaggccca gattgcgagc aatattgcta gatcaaggca gataattaat agagtcagcc
    25141 agattggcca tctcattacc tcacgcatga ggcgagagta cttccttgca ttagatagcg
    25201 cttgcctatt gacagggtaa tcttcacccc atgtcgcgcg agctaaatat tcaagttccc
    25261 ccgggagtta aaatcccgcc tagacatcct gatgctccgg caagtggcaa attactcgga
    25321 tctcataatc ctgattgcta tggatgcggt cctaaccatc ctgcaggatt acatcttgtt
    25381 gcctatgcag gagagagtat gagcattact gcgcaattta cagttactga tttacatcaa
    25441 ggagctccag gtcttgccca tggcggccta ctttcatgcg cttttgatga agctttagga
    25501 aaattaatgt ggttagttag agagccggca gtaactggaa aacttgaaac gtcatttctc
    25561 gcgccagttc cagttggaag cacattattt atcaatgctg agataaccgg ccaggatggt
    25621 agaaagatct acaccagagc tgagggcaga attggttcag atagtggggt tttagcagtt
    25681 gaggcagcag caatatttat cattgttccc atggagcatt tcatcaaaca tgcatcagcg
    25741 gaattcaagc aggtattaaa agatcgaaga gatttatcta tcgatactga gtttgagatt
    25801 aatccatgaa agtactaata actcaattag ataaagggct tcccatgccc tcctatgcaa
    25861 aagctggtga tgctggagct gatatttatt cagctataga tatcactatt gagcctggca
    25921 tgagagctct tgtgccaact ggtattgcca ttgcgctaag tgatggatat gccgcctttg
    25981 ttcatccccg ctctggcctt gccattaaat atggcgttgg gcttgtcaac gctccaggaa
    26041 caatagatgc gggttatcgt ggagagattt gcgtaatagt catcaatcat gatcagagcg
    26101 agagttttga aataaaacgt ggcgatcgca tcgcgcaatt agtttttcaa agagttgaac
    26161 gcgctgaatt tgttgcagtc gaagaacttc ctgggtctgc tcgaggtgga gctggttttg
    26221 gttcaagtgg gcgctcatga gtgagagcga agataaagca aaaatcctct ctgctatggg
    26281 gggagtaaaa gggctaatag attctggact tcctgctctg ctatttctta tcgcatttaa
    26341 tattacaaag gatgttagag attcagccta cgccgccctt gctctctcgc ttcttctaac
    26401 gctggttaga ttggtaaagc gtgaaaccat tcagcatgca gtttcagggg tgattggagt
    26461 tggaatatgt gcttggctct ccatgcgaac tggaaaagca gaagacttct atctgccagg
    26521 actttggacc aatgcaatct atggatctgc ttatttagtt tcaatacttc tgcgctggcc
    26581 agtaattgga ctcattattg gtccacttct ggaagaaaac tttagatggc gtaaagatcc
    26641 tgcgcgaaca aaggtctata tcaaggcaac ctggctctgg gtaggaatgt ttgcaatccg
    26701 ccttgttgtg caatacccgc tatatgtcag tggcaatgtt aatttacttg gaacagccag
    26761 gttggttatg gggtatccac tatttattgc tacagcctgg gcaacttgga tagtaattaa
    26821 gagtggtcca aagctaagag atgatttaag aattgataaa gcgagctaat cctttataaa
    26881 gcaagccttt agtgagactt cggcctcggc agtggttaca aaaattagct catcgccagc
    26941 tgcaaaaaca tcatggtctg ctggcgttat gacatgacca tctctgataa tcgctgcaag
    27001 tgatgcatct tggggaagtg agatctcttc aacggatttt ccaatgcaag atgaggtatc
    27061 gggaagagtt aactcaacta aattagcctc tccagcttta agcgagaaga gtctgacgat
    27121 atctccaact gatacagctt cctcaactag ggccgcaata attcttggcg tagaaacagc
    27181 cacatcaacg ccccaggagg aatcaaaaag ccactcattt tttgggtgat ttactcgtgc
    27241 caccactctt gggactccat attcagtctt tgcaagaagt gatgacacaa gatttacttt
    27301 gtcatcaccg gtggctgcaa ccattacttg gcaggtatca agcttggctt tatcaagtga
    27361 actgatttcg caggcatcag ccaggagcca ttcagcagat ggaacgctct ccatctttag
    27421 ggactttgga ttcttatcga taagtaagac ctgatggcca ttttcaatta gctctcttgc
    27481 aatggctcgt cctgcattac cagctcctgc gatagcaatt ctcatcttca ctcaccactc
    27541 ggtggaagag caagagatgc ctctgttgcg gctaaattct tatcttctac cattacatga
    27601 atgacatcgc cttcttgtaa gaccgtatgt tcattgggta ttaagccttc accaagcctt
    27661 gtaacaaatg caacacgagc atcggtatgt tgttctatta atgaaacagg ccttccgtac
    27721 caatctcgat gcagagcaat ctcagctaac tgcacttttc ctgatgcatc ttgccactca
    27781 gagcgagatc cttgcggaag aagccttctc ataatttgat ctgtggtcca aagaactgtg
    27841 gcaacagtag gaattcctag cctttgataa atctcagctc tacctgggtc ataaattcga
    27901 gccacaacgc ttgctactcc ataggtttct cttgcaactc ttgctgccaa gatatttgaa
    27961 ttatcgccat ttgaaactgc ggcaaatgca tcagcccttt gaattcctgc ttctaacaaa
    28021 gtgtcgcgat caaagcccat tccagccaca gttagcccat tgaaatctgc gccaagacgg
    28081 cgaaaagctt cacgatcttg atcgattaca gcaacgctat ggcctagagc ctgaaaatct
    28141 tttgccaggg tagaaccgac tcggccgcaa cccatgatca cgacatgcac aaaaggagaa
    28201 tttacacctt taggctagat tccctctaat ccacgcgagg gatagcacga gaattaagtg
    28261 cttcaatgaa cttttttaga aaggtgcgcg agtgcaaagc ggtgatcaat tagaagtcga
    28321 aatcacagct attgcgcacg gcggccactg catagctcgc tacgaaggaa gagttatttt
    28381 tgtaagacat gcaatccctg gagagcgcgt gattgttgag attagcgatg tgacaaagag
    28441 ctttgctaga ggaaactgca tctcagtaat caaaccttct aaacatcggg taagtccgcc
    28501 ttgttcgtat gctcgatttg atggatgcgg gggatgcgac tttcaacaca tcgatcttcg
    28561 ttatcagcgg gagttaaaga gcgaaattat caaagagcaa tttagccgtc ttgccaaaat
    28621 ggaggttgat gtagaggttg aagaagttaa accgaattta cattggcgca gcaggatgga
    28681 attcaccgta tctgaaactg gcaaggttgg tctctatgcc tcgcgctcca atcaaatagt
    28741 tgaaattgat aaatgcctaa tcgctcatga agatattgat atagcgaaga tcaatcagat
    28801 gaagttgccc aaatcaaagc gggtagatgt tgcaatcact agcaagggca agagcgcggt
    28861 agtgatagag ggtcgagaga acttagaact tatccaggag caggtagatg gcattgaatt
    28921 ctcgctaaac ccaatcacct tctggcagag ccatcgcatg gcgccgactg ttttaagcca
    28981 agtagttaag gattatgttc aagcaaagcc tgcagatcac atctttgatc tctacggcgg
    29041 agctgggcta tttagcgcag cccttttatc tgatcttgga gttgcaggaa gagtcacctt
    29101 aattgaatcc gatgagaatg cgattattga tgccagacga aactttgccc tagaaccaag
    29161 agttgagata gttgaagcaa aagttgaagc aagcttgaag aaatatcgcg cagcaaatgt
    29221 agttctcctt gatcctcctc gcgctggagc tggggagaga gtcatttcaa ctatcacctc
    29281 acttgctccc agaacgattg tctatgttgc ctgtgatcca gcctcacttg cccgagatag
    29341 cgcctatctt tcagcgcagg gatataagtt ggatcaaatc agggcctttg acctctttcc
    29401 aatgactgct catatggagc ttgtggcgcg tttcattatt tcttgagtaa ttctctagta
    29461 attcgtcttt gagcacttta tgcgacaaca gttaagatgg gctcatgagc aagaactcct
    29521 tcggcgcaaa gagctcgctt gaagtatcag gcaagagtta tgaaatattt gatatcacca
    29581 aacttgctca agcaaattct cttccctttt ccttaaaagt attactggaa aatctccttc
    29641 gcactgaaga tggtgcaaat atcacagcca agcagatcga agctcttgct tcatggaatc
    29701 ctgaatcaat tcctgatact gaaattcaat tcacaccagc gcgtgtgatt atgcaagatt
    29761 tcaccggagt tccctgtgtt gtcgatcttg ccactatgag agaggcaatt gcagaacttg
    29821 gcggagatcc atcgcgtgtt aatcccttag cccctgctga acttgttatc gaccactcag
    29881 ttattgcaga taaatatgga acggcaagct ctttcaaaga aaatgttgat attgaatatg
    29941 agagaaatca agagcgatat cgcttccttc gttggggcca gagcgccttt gatgaattta
    30001 aagtagtccc acctggcacg ggcattgttc atcaggtaaa cattgaatat cttgcaagag
    30061 tagtaatggt cagagctgta aatggtgcgc agcgggcata tccagatact gtggttggaa
    30121 cagattcaca caccactatg gtcaatggcc ttggagttct tggttgggga gtgggcggca
    30181 ttgaagcaga ggcagccctt cttggtcagc cagtttctat gctcattcct aaggttcttg
    30241 ggtttaagtt aagtggagcg ctagcagttg gaacaactgc aactgattta gttttaacaa
    30301 ttactgagat gcttagaaag catggcgtag ttggcaagtt tgttgaattt tatggcccag
    30361 gagtgagcgc gcttccgatg gctaatcgcg ccaccattgg aaatatgtct cctgaatatg
    30421 gctcaactgt ggcaattttc ccaatagatc aggaaaccat taagtatcta gagttaaccg
    30481 gaagaagcag agaacaatta gagctagtag agaaatatgc caaggctcaa ggactatggc
    30541 atgacccatc tgcagagcca agatactcag agcgattaga gctagatctt tcaagtgtcg
    30601 tgccatcaat agcaggaccc aagagaccac aagatcgagt ctccctaagt gatgccaaga
    30661 gttcctttgc caaagttctt ccttcttatc tttctagcaa tactgctact aatccaattg
    30721 ccattgaagt aaattcaaga aaaggatcta ttaccaatgg ctcagtggta atagcttcaa
    30781 ttacttcttg cactaacact tcaaatcctt cagtgatgat tggggcagga ctactagcaa
    30841 agaaagctgt agaaaaaggt ttgatgagta agccttgggt taagaccaca cttgcgccag
    30901 gttcaaaggt agtaactaac tactacgaaa aatcaggatt aactccatat ctagaaaaac
    30961 tcggatttaa cctagttggc tacggatgcg ttacctgtat tggaaactct ggtccattgc
    31021 ctctagaggt tagcaaagca gtaaatgaca atgatctctc ggttgcagcg gtgctttctg
    31081 gaaatagaaa ctttgaaggt cgaattagcc ctgatgtgaa aatgaactac ttagcatcgc
    31141 ctccgctagt tgtagcctat tcaattgctg ggactatgga tcatgacttt gataaagatc
    31201 ctctaggcaa agatagctct ggaaacgatg tgttcctgaa agacatctgg ccaagtatgc
    31261 aagagatcca agacgtaatt gattcagtag ttacctcaga tatgtttaag aaagattatt
    31321 caagtgtatt tgctggagat catcactgga cctcacttgc aacccctgaa ggaaagactt
    31381 ttgaatggga tcctaaatct acttatgtaa gaaagccccc ttactttgag tctatgccta
    31441 gaaatccact tcccgttact gatatttcag gtgccagagt tttggcaaag cttggtgatt
    31501 cagtaactac tgatcacatt tcaccagcag gctcgattaa ggttgattct ccagccggaa
    31561 aatatctagc agaaaatggg gtagaaaaat ctgactttaa ttcctatggt tcgcgccgtg
    31621 gcaatcatga agtcatgatt agaggaacgt ttgcaaacat tcgcttgaag aatcttctac
    31681 ttgatggtgt tgaaggtggc tttactagaa actttattga tggtggtaag cagagcacta
    31741 tctatgatgc atcaatggca taccaagatg ccaacattgc tctagtgatt ctggcaggaa
    31801 aagaatatgg ctctggatct tctagagatt gggctgcaaa aggaaccgca cttcttggag
    31861 taaaggcagt cattgctgaa tcatttgaaa gaattcaccg ctctaatttg attggaatgg
    31921 gagttcttcc tctgcaattt ctcgatggtc aagatgccaa ttcattaggg ctaagtggcg
    31981 aagaagtatt ttcaatatct ggtgttactg cgttaaacca aggtcgtatt ccaagtattg
    32041 ttaaggtgac ggctggagat aagagctttg aggcaaaggt acgcatcgat accccaggtg
    32101 aggctgatta ctatcgccac ggtggaatca tgcagttcgt cttgcgctct cttctttctt
    32161 agttcaccga tagaatccag acatgaaaca ccttagccgg attaaatctc ctggcgactt
    32221 ggcgcgcttt agccaagaag agctaacaga gttagcggca gagattagag cttttctaat
    32281 cgagaaagtt tcaaagtcag gcggacatct agggccaaat cttggaattg tggagttgag
    32341 tattgcgctt catcgcgttt ttgaatctcc ccaagatgta atggtttatg acacaggcca
    32401 tcaaagttat gtccataaga tattaaccgg tagagctgca gggtttgaaa agcttcgtca
    32461 gcgcggagga cttgcaggat atccaagtag ggcagagagc gaacatgatg ttatagagaa
    32521 ctcacatgct tcaaccgctc tctcatgggc tgatggaata gctcgtggtt ttgctctgca
    32581 gaatcagagc gatagaaccg ttgtagcagt agttggagat ggagctctaa caggtggaat
    32641 gtcttgggag gctttgaaca atattgccgc ttcagatgat ctaaaacttg taattgtggt
    32701 taacgataat gaacgctctt actcaccaac tattggcgga gtagccacct atctttcaac
    32761 acttcgcgct acaaaaggtt atgaaaaatt ccttgattgg ggaaagcaag tattggaaaa
    32821 gactccggtt gtcggaaatc caatctatga aactctccat ggaatgaaaa aaggtattaa
    32881 ggatattgtt gctccgcaag gaatgtttga ggatctagga cttaagtatt tgggaccagt
    32941 tgacggtcat gatattccag cactggagcg tgctctcctt caggcaaaga aattctcagg
    33001 gcccgtatta gtccatgcca tcactgaaaa gggacgggga catgctccag cgctcaaaga
    33061 tgaggcagag aagtttcatg cagttggggt cgttgatccc gaaacagggg agccgctatc
    33121 caataatggc gccacctgga cgaaggtatt ttctgaagcg atagttgaga tagggcgaga
    33181 gcgaaaagat gttgtagcaa ttactgctgc catgcttggt ccaacggggc ttgatcgctt
    33241 ccaagctgaa tttccagagc gaacatttga tgtgggaatc gctgaacagc acgctgtcac
    33301 ctcagctgct ggtatggcct acaccggcct tcatccagta gttgcggtct attcaacatt
    33361 tttaaatcgc gcatttgatc aattgctatt agatgtggga ttacataaag ctggagtcac
    33421 atttgtttta gatagagcag gaataactgg cgatgatggg ccgtcgcatc acggaatctg
    33481 ggatctagca cttactggaa taattccaaa tgcatttgtt gcagcgcccc gcgatggtgc
    33541 gagattaaag gaatcactta cagaagctgt tgcaatctct gatgcccctt caattattcg
    33601 cttcccaaag ggagcaattg gaaaagattt gccagcattt gaaagacgtg gcgggctaga
    33661 tgtcctctat cgcggtgaga gttcagatat tcttcttgtg agcattggcg ctatggcacc
    33721 tcttgcaatt gatgcggcag ctcaagcgtt tcgtgaaggt gttggagtaa ctgtcatcga
    33781 tccactctgg gttaagcctc ttcctgcaga ctcacttctt gccatggctc caagatttaa
    33841 acgagtagtg gtgttagaag atggaattaa acatggcggg attgctagca ccttatctga
    33901 gctatttcgt gaaaatggtt tagatctgcc aattcattca attggagtgc ctttggaatt
    33961 tattcaacat tcaaagcgcg ccgaaattct gcaagatatt ggaatcactt cacagagcat
    34021 tagccgtcaa ttagttggct gggcttcgtt aaatgttaag caggaaggga tgcaacttgc
    34081 ggcggatgaa aacgctgacc gcaaaccgct tcactaattc cttctctatc aagataaggc
    34141 aatattccgc ctaaatgcat tggccatccc gcccctagca acatacagag atcaatttct
    34201 gatgcagtgg caacaacgcc ttcatccagc atcattcttg cctcttgagc aagggctgat
    34261 agagctctgg tgcgaacttg ctctgcagtt gatggcttat ctcccttttc aatcagagcg
    34321 agggcttcgg gattagcgct aagcgagcct ttctcatctt tgacatagaa agttttaacg
    34381 cccttctcca ccattcgaga aacagttggt gagatgcgat agcgcggacc taggttggca
    34441 tttaaagtct tggagacatg aagggcaact cctggaccaa ctaggccaaa tagttcaaat
    34501 ggcgacattg gaaaaccaag aggtcgcaga gcgttatcgg ctacatctgt tggagttcct
    34561 tcatcaacag catctgtaac ttcgcccata aatcttgtta gcaggcgatt aaccacaaag
    34621 cctggagcat ctttacagat aatcattgtt ttctttagct ctttaccgac gttaattgca
    34681 gtagcggtag tggcatcatc agtttttgaa gttcttgcta cctccaagag tggcataaca
    34741 gctactggat taaagaagtg gaatccaaca acgcgctctg gatttttcag accttcgccc
    34801 attttttcaa ccgaaagcga tgaagtattg gtagctagaa cgcactcagc agagattatt
    34861 gcctccagat ccttaaacaa cttctgcttt attgagagct cttcaaatac tgcttcaatt
    34921 atgaagtcac agccagcaaa agcagattgg ctcacagatc ctgagattag agacgataat
    34981 ctagctgcag cctctgggct taagcgcttt ttctcaacta acttagtaat ttcatttcta
    35041 acccaagcaa cacctttatc aacgcgctct tgatcaaggt cagtcataac tactgggact
    35101 tttagatttc taatcattag aagtgctaat tgagatgcca tcaagccagc tcccacaact
    35161 ccagcttttg tcacctttct agcaagagca gcctttggag ctccttcaac tttcttacgc
    35221 ttcttttgaa tcagattaaa tgcatagagc gaggccctta aaggattgcc cataactaga
    35281 tttgccaaga tttcatcctc ggcatcaaaa ccttgatcca gggtgttact tcttgcagca
    35341 gagatgagtt ctaaggcgcg ccttggggct tcaatatctg ctccgccata ttttttggca
    35401 attgcagctt tgcctgtttc taaagcttta tcccaattgg gatctgttga ataatcttta
    35461 cgctctatct tctcttttcc ggaaacaatt ctggcagcaa aggcaattga tttttcgaga
    35521 aaatcagcag gctcaaacac tgcatcaaca actccaaggc cctgggcatc tttagccttc
    35581 atcatcgtat tgttatttaa agcatttaaa ataattactt gcacagctct ctctggccca
    35641 attagtttag gaagcagtgt tgctccgccc caaccaggca ctaagccaag gaaacactcg
    35701 ggcaaagcgg taagtgcagt tgtggccatg gttcgataat tgcaatgcag gcctacttca
    35761 aggccgccac ctaaagccag cccattgata aaggcaaagg tgaccttctt gcttctgccg
    35821 agtcttttga agatgtcatg tcctaatttt ccaaacgcta gtgattgctc tcgcttggca
    35881 acatatgaga ttccagagag atctgctcca gctgcaaaga taaagggttt tccaataatt
    35941 gctatcgcta ccgcatcgct actttcagct gcggtaattg catcatttag tgcgtttaga
    36001 gattgaattc caaaggtatt tggcctggtg tgatcatggc cgttatccaa agttattaag
    36061 gcaagggagc cggttgcgcc gaaagccgat agatcaactt ggcgcaagag agcgtttgtt
    36121 actacttcat ctgggggagt tgttatctct gccatattac ttatgctccc tttttgtaat
    36181 ttgggttctc ccagatgatg gttccaccca ttccaagtcc aatacacata gtggtgatgc
    36241 catatctaac ttctgggtgt tctttaaaac cctcagcaag attgagaatc aaacggattc
    36301 cagaggatgc aagtggatgg ccaacagcaa ttgctccgcc ataaggattc actcttggat
    36361 catcatctcc aattccaaag tagtcaagga atgagagcac ttggattgag aaggcttcat
    36421 taatttcaaa taaaccaata tcttcaatct ttaagcctgc cttatcaaga gccttaagcg
    36481 ttgatggcac aggcccatat cccatgatct ctggttcaac tccagcaaat gcaaaggtaa
    36541 tcattctggc ctttggtgat agtcccagct ctttagcctt actctcgcta gcaagaagcg
    36601 ctgcagttgc accatcgttt agtcctgccg catttccagc agtaactctg ccctgaggac
    36661 gaaatggagt ctttaacgca gcgagtgctt caagtgttgt tccaggtctt ggtggttcat
    36721 caactgttgc aacgccccaa ccttgctcag cgtttctaac tgcaactgga attaaattag
    36781 gttgaatctt tccgctctta tatgcagcag cagttttttc ttgagagcga agtgcgtatt
    36841 tatctgcacg ttctttagtt agatgtggga atttatcgtg cagacgctct gcagtatttc
    36901 ccatcgagag cgcatcggta tcaactaact tctctgccaa atatctagga tttggatcca
    36961 tgccatcacc cattgggtga ttgcccatat gttcaacacc gccagcaatt gcaagatcgt
    37021 atgcgcccat tgcaatagat ccagcgagag ttgtcactga agttagcgca cctgcgcacc
    37081 agcgatcgat tgaatatccc ggaacagtat ttggaattcc ggcaagaagt gatgcgcttc
    37141 taccaatgtt cattccttga tcgccatatt gagtagcagc agcaattgct acatcatcaa
    37201 tttcagatgg cttaactgat ggatttcgct ccagcaggcc gcgaatagtt cgaacaataa
    37261 tgtcatcggc gcgggtgccg gcatataaac ttccagcttt tccaaaaggg gagcgaacag
    37321 catcaaggag aactacattg gttgaggtgg tggcagtatt cataccacta ggttactggt
    37381 cagtaaccta ttagcaaagt gcttactagc tgttaactct gctttacagg ggtcagactc
    37441 ttggcaggtc ttaaatatag atagagcgtt ccaagaatgt aggcgccgta agcaaacaat
    37501 tgcaaccacg acatcgtggt atcaaatccg attgttccag caagcagtga tcccataatt
    37561 gaatctgccg ccatccagga agttacatcc caggcatagg attcttcccc tggaagccac
    37621 cctaattctt ggaattcatg gattccataa gataaaactc ctgcggcaac aataatcaat
    37681 gcgacgcccg taatcgtaaa gaacttagaa agattgaact taatcgccct gcgatatatt
    37741 gcgtatccaa gtaacaccgc aagcagcaac cctgaaacca aaccaatcgt ggcaggagtt
    37801 tttgatcctg cgacttgaaa atttgagtag acaaaaaggg ctgtctcaag accttcgcgt
    37861 gcgactgcga aaaaggctgc tgcggtgagg gctaaggggc ctaccattgc agccgtctca
    37921 accttttcat ggagggtatc tctaagggct ctggcagttg ccttcatcca aaagaccatc
    37981 caagtaacca aggcaacagc tacgaatgaa gtagttccag caaaaaactc ctcgcctctt
    38041 tcagaaagct cggctgaagt aaatgccaag aaaccaccga gcgcaaaaga gatagcaaga
    38101 gcaacaccaa ctccgctcca taaaggaggt aggagattgc ggcggttggt tttaaccacg
    38161 taggccacga gaattccaac aatgagggca gcctcaaggc cttcgcgcag cgctattagg
    38221 aaattgctaa gcatgggtag agattaaacc tattctaccg atttacgcaa ctctaatgtt
    38281 gcgtaattca tctcactcct gaggctcaga atcatcctga actggggctt cagagggcaa
    38341 tggctcccct tctccgaggc ttttcgccag agatcgagag cacttttcta tctgccactc
    38401 tcttgctcca ttaagcctta actgcgtttc aactcttgcc agcagagcct cactcatttt
    38461 atggatttgt tcacttcctt gatcaaaaca gatttttctt accaactcag gccccatgag
    38521 gttttctagg gggagattaa gttctagagc aatttcattt aacttagctt tggcatggct
    38581 tatatgagca taggcgattg gaaattttgc cttccaaatc tttactggcg gaagggaatc
    38641 gcttctgctt cgaagttctg gccattgctc ttgagttaag ctctgggctg caagaaatgt
    38701 ctctaaccaa gtttcaatgt aactcttttg cgtttcgttt cttattcgcg ccttagcttc
    38761 gctaagtttc ataaattcat ctttactctt cacattagat ttagccaaag caatgatcac
    38821 cgcatctgat aagagccgtc ctggagctag gtcaattgaa gctgcaatct catcgcgcac
    38881 ctgccagagc tgcctaatga ttgccagttg atatcgagat ttaatctcat gcattccaga
    38941 agttcttctc caaggctctt ttcgtttttg cgggggagga ctctttaata ttgctgcaaa
    39001 gtcttgcatt gcccacttta attttccttg tgcctcaaga agttctgcta ctttatctct
    39061 aagatcaata agtagggcca catcaagtgc tgcataatca agccactcct gctctagtgg
    39121 gcggattgac caatcaacag ctgagtgctc tttagcaagt gagatctgca ataaactttc
    39181 aactaaatga ccgaggccaa ctcttggaca acctgcaatt cttgctccta attcagtatc
    39241 aaatagctct ttcggatcta tgccccaatc gcgaagacag ggcagatctt gagtgctggc
    39301 atgaatgatt gtttcctcgc ctctaataac ttcatttagt tggctaatta gagggctttg
    39361 gttaaattgg attggatcaa ttagatgtag gcccccgccc cttcgatata tctgtattag
    39421 ataggccctt gagctatagc gaaatcctga ggcgcgctcg gcatcaatag caattgggcc
    39481 gtgaccagat ttgagttggt ctattgctgc agctaagtga tcctcggttg taattagttc
    39541 tggagttccc tgttctggaa cctttagggc gcgaagattt gtctcttgct cactcacagt
    39601 taattaacgc ttttgcggaa gtgaggccac accctcagga agaggggcaa gacctgcagc
    39661 gcttgccatt agttctagcc aagcatggac atggctcatt aactcttcag gattatctgc
    39721 agtccatgaa gctcgaatct ctatttccga tgattcgtca tgtgatgaga gttctccaaa
    39781 tgatgcgctt gcaactctag ttactgttcc gctgggctgg ctataacttg caccgctgtt
    39841 ttttagcgca tcaataaacc aagaccagcc aacatcgggc aacatgggat ctgattgaat
    39901 ctcaatatca agggctgagc gaacaaaggt gacgcaacga aaagttccat cccaagattc
    39961 ttgtccatct ggatcatgaa ggagaacgaa tctgccagtt gcgatgtcat cttcgctatc
    40021 aatatcaagt gctaaatcgg cggtgatggc aaaggcatag ggggcaagtt tttgcggcgc
    40081 tggtatttca gagatggaga tttcagggcg cgccttaatg gcttttatat cttcgataaa
    40141 agtatcgaag tttcgattcg ccgctttcat aaggataagg gtaggcaaga gttaaatgag
    40201 tcagcgggag gcgcgagggc ttatgaggcg ataaccttga ttaatgggct cattacttgc
    40261 gctagcttcc agcctgctct ggggaactgc ggattactta gctggaaacc tctctaagcg
    40321 ctttaaagca atcgccgtca cagcgatctc gcagatattc ggacttatct ttggccttgt
    40381 aacaattcta ttttttgcag atttcattag accaaatctt tcaatgagtg gctatttcat
    40441 cccaggagtt attgccggca ttgctggttt tatcggccta atcgcttttt ataccggtct
    40501 tgctaccgga cgtatgggag ttgtttcacc gattagttcg ctctcagttc taattccatt
    40561 gttttttgcg ctggcaagag gtgagcgacc tacctctctt gagttgattg gaatagtaat
    40621 tgctatcgct ggagcattta tggcaagcgg gcctgagata agaaatggtc ttccagtaaa
    40681 gcctctcttg ttagcagttg tggctgccat tggttttgga actgccctaa catttattgc
    40741 aattggatct gagactgatt cccttcacac catgacttcg atgcgagtag cttcagtatc
    40801 aatctgcatc ttgctagcga ttagatatcg aagccttggt ggaattccaa ggaaagaatt
    40861 tccactattg atatttattg gtgttgcaga ttttctagct aactttctcc ttggggtagc
    40921 aactaccaaa ggcctagttt ctatcgctat ggttctaggt tcgctatttc cgatagtgac
    40981 tattctttta gcatataaat ttcttcatga gagattagcc aaagtccaat acttgggaat
    41041 tgcttttgcg ctaggcggag tacttttaat cgctgctttt taacttaacc ctccagcttt
    41101 caaagctaac gccatcaatc atctcgatag attgcctctc gtaattctta agtaaattac
    41161 tctcatcaaa gaagtggcca tcaccatctt tattaactct ggtgatgtag agaagatcaa
    41221 ttaggctctc ggcaattaaa ctcgagatga aattaacccc gccctctatc aatactgggt
    41281 agccctgctc taataaagcg cgattaatta gccaggctgg atcttgatta aagatgaatt
    41341 tactaccaac ggctccttcg ttaagcgtgc gagatgagat atagagcgga aggctcaatt
    41401 tagagtaggg ctcatttcta taactctgcc cgccaattaa tatcgccttg gctagcgtgc
    41461 gaatctggtg gaatctaatt cgatcacttt tgggacttaa ctcccttgag ctacccccaa
    41521 ggctggtggc cagattcctt gccaggagaa tattggccat cgttttgacc tctggtcctt
    41581 gcttcataag tagcacgcta ccgcccaagt gatgatgtca gtgccgctct ctatcgttgc
    41641 cttcatgatt attgattgcg atagttgcat catgcgagat atcgcatgta aagattgcgt
    41701 agtttccgtc ctaatttcag ccccaagtaa tcagagccat gagccttctg agcttggtct
    41761 tgaggaggcg cgtgtaattg atcttctctc ctcacgggga atgattcctc ctcttcgcta
    41821 cgctcaaatt gagacagaaa gtgataattc acaggcaaat caagcccttt caggttgagc
    41881 tggccttact ttcgcagtta gcctaacgcg atgaaattgg ctcttcggtg gctaacgcct
    41941 tatttggctt tagttttagc tatcaccctt cttccaacat ctagcgctgc cccaagcgct
    42001 gatttagtcg cagtccgggc agaggttgaa cgccttcaag aagatgccgc tgaagccgga
    42061 gagaatgccc aagctgccaa gattcaatta gccagactta aatcccaatt ggagtcagtg
    42121 cgccaagagg cagatcttca aaaagctagc gttgaatcga ttgaaaaatc actatcagca
    42181 attgcagttg cccgatataa atcaacggga ctcggtgaag gtttagaact gctcttctcc
    42241 tcggatccat ccctttatct ctcagctgct ggctcgctag aggatgtaac tagaaaacaa
    42301 tcgctagaac ttcgaaagta tgccactgcc aagcagcgcc ttgatgccac atcactaaca
    42361 gtcagcgaca aagttagatt agttcaagcc gcagaggcgc gctatcgcgc tcaagcagct
    42421 caagttgaag ccaaacttgc gcaagccgag aaattattat caaaactgga gaaagcagat
    42481 cgagagaaaa ttctcaagct tcaggcgatg gatgcagcgg agcgacagag atattcaaaa
    42541 gagcaggcaa agcttgcaaa tgccgtatca ggaagagctg gaactgccct tcgttacgca
    42601 gtgcaacaaa ttggagataa ctatgtttgg ggagcagcag gtccaattcg ttgggattgc
    42661 tcagggctaa ctatgcgctc tttccaaaga gctggggtta aattgccaca ctcctcccgg
    42721 gcgcagtttg gctacggaag atcgatatca agaaataatc tccaagcagg cgacctagtc
    42781 ttcttcggca ctcccatatc tcacgttggg atttatattg gcaagaacaa gatggttcat
    42841 gcgccaagac ctggagctaa agtgcagatt gcagagtttg gaaactactt tggtcggaaa
    42901 aagtatgtag gagcgcgtcg actctagagt tgatcagtag aatttaataa tgccttcaat
    42961 attgcttctg accaatgact ttggcccgcg cgcaggagga atagaaactt tcattattgg
    43021 cctacttgaa agaatgccaa aaggcgaagt tattgtttat acctcaaagc aaattggcag
    43081 tgagagcttt gatcagaagt ggcttgaaaa ctttggcgtt agggttattc gcgactcatc
    43141 caagatattg ctgcctaccc ctcgggtaat aagggcagtt agaagagtta ttaagcagag
    43201 caaagtagag aagatttggt ttggagctgc agctccactt gcaatctctg caaggttttt
    43261 aagagtaggt aatgtcaaga aaattgttgc tctaagccat ggtcatgagg tctggtggtc
    43321 aaaggttttt ccctttaact ttgcaatgaa ggagatttct agaagtgttg atgttctaac
    43381 ctttctaggc gattacaccg gatcagtcat tggaaagcac tttaaagata aggctaagtt
    43441 agtaaaaatc gctcctggaa tcgatgtaaa tcactttaag ccagagcgag atccatcaaa
    43501 ggttaacgcc ttcagatctg aattaaagat tggttctgag cccttaattt tatcggttgg
    43561 aagattagtc catagaaaag gtcaagataa gttgatcacc gcgatgccta agattctggc
    43621 taagcagcct gatgctaaat tgctatttat tggccaaggt cctcgaaaag caaagcttga
    43681 ttcgctagtt gcaaaatatg gcatagaaga ctcagtaatt tttcttggaa gtattgccta
    43741 tgcagatctt ccaaagtatc taaacgcggc agatttattt gtaatgccat ctagatctcg
    43801 cctgatgggt cttgaagtag aaggtcttgg aattgtttat ctagaggcaa gtgcctgcgc
    43861 tctgccagtt attgctggaa gctctggggg agcccccgat gcactcattg atggcagaac
    43921 tggatatgtt gtagatggca tggatatcga tcagattgct aaccgagtgc tttccttaat
    43981 cgataatccg aaggtagcaa aagtgatggg cgaagccgga agagcttggg ttgagagcaa
    44041 ttggagttgg gatatttggt caagcgcctt taatgaacta ctggaaatat cagggaagta
    44101 ggccagtttt cctagctgtg tttatacatt gaacgcgatt ttttgcgcca aacttcttat
    44161 aaattgatgc taggtgagtc tttactgttg attcggttac aaataattga gctgctattt
    44221 caaggacagt atcaccggtt ggaagctttt caaggatctc tagctctctt ggagtaaggc
    44281 ctacttctcg atttttagca tcgattgctt gcgtcaactt tcttgcggta aaacttaaag
    44341 gcttaactga acttgccttg atagcgctta gtagctcact gcttggcgat gctttatcaa
    44401 catgagagct cgcccctgat tgcattgatg ctagaacatg ctctggcata ttagacatag
    44461 ttaaaaccac tattccaata cgctgtgaga gagatctggc ccaagccacc acttcaaggc
    44521 cattgccatc aggcagattt aaatcaacaa caattacatc agggtttaga tgcgatatct
    44581 gagcaaatgc ttctctctta gatcctgctt cgccaatcac acagatttct tcgtcgcgag
    44641 caattaaacg gcgcaggcct tctctaacca tagtgtgatc atcgactacc aataccttaa
    44701 gcattattgc ccccagattt cctaatctgg aatttaaagc tactaccgtc actctttgag
    44761 gagctggagt aggaagaaga gattttgatt agtagttcat caatacttgc taaaccaaat
    44821 cgaaaatcct taggtttaac tccaccaatt ccatcatcgc cgatttcaaa ctctatgctc
    44881 tcttcattct ctttaaagtt gagatagaag caagttgcct ttgagtggcg agatgtattt
    44941 cttgctagtt ctaaaattat ctgacttaag gcctcttcaa cctttggatc aaattctgga
    45001 taggtaaagt cagctgctag cttgatctca cctaatatct ctactacttg cttggaaagt
    45061 tgttgccttg aggtgagctt taattggtag atctgatcgc gaaaagactt agtcgtttgg
    45121 cttatagaaa atcgaatctt gcgcagcgct attcgatgct tcttgcctaa atttgcatca
    45181 ccaattaatt catcgagctg atagccaatt acagctagat cttgggccaa agtctcatgg
    45241 agacggcgag ctaaatctag gcgaagagtg ctctcttggg tctctggttt agatttagcc
    45301 aaagagttcg gcgatctcgg ccgcgaactc tttatggact acgtgacgct taactgacaa
    45361 cttggcagtc aaatgacctc cggcgatggt gaaatcaacc ggcaagattg tgaacttacg
    45421 aattgactca gcacggctta ctgccttgtt agcttcatca actgcagttt gaatgactgc
    45481 aattagatca ggatcattgg tgagctccgc gagagttgca ccatccttct tgtttgcagt
    45541 gatccaaccc ttgagtgcat cagggtcgat agttactagt gcagcgatat atggctggtt
    45601 atcgccaacc actatgcatt ggctaactag tggatgggcg cgcaggcgat cttcaagaac
    45661 tgcaggggca acattctttc cacctgatgt aacaatcagt tctttctttc gacccacgat
    45721 atagaggaag ccctcttcat ctagttttcc taaatcaccg gagcggaaat atccatcttc
    45781 ggtgaaaact tctttattag ccgcatcatt ttgccaatac ccgcgcataa caattgggcc
    45841 cttaattaag acttcgccat cttcggcaat cttgattgtg gttcctggga tgggttttcc
    45901 aactgagcca accttatgag cagtagataa attaagggtt gcgccagcgg tggtttcagt
    45961 taagccataa ccttcaagga ctctaactcc agcgccgcga tagaagtggc ctaaacgctc
    46021 acctagtgga gcaccacctg atatcgctgc ctcaaccctt ccgccaagac catgtctgat
    46081 cttggaataa accaatttat caaagagacc atgttttaac ttaagggtta gagacgggcc
    46141 gccattatcg agtgatttgc tgtattccac agcgatagct gcagccttgc gaaagattgc
    46201 accctttcct gctgcgtcag cttttgcctc agctccgtta taaacctttt caaaaattct
    46261 tggcacagca agaacgaagg ttggtttaaa tgatgcaagg tcgattggta gtctgccaac
    46321 aggatctgga cagtgggcaa gatgtagacc tgcgcgaata gcgccaatct gcaccatgcg
    46381 accaaagacg tgagcaacgg gaagaaatag cagcgttgaa ccaccagctt tcataaataa
    46441 gtcagcagcg cccataacta catttccaca ctctgataaa aagtttccat gggtaagtgt
    46501 tacgcccttt ggttttccag ttgttccaga ggtatagata agtgttgcaa gagaatctgg
    46561 agttagcgca tttctgcgat tatcaatttc ttgatcagag atatcgcggc cgagatatgc
    46621 aagttgcgct agaacattct cagtcataac ccaacaattc ttgacattgc tatttagaac
    46681 gggggttaca atttcgcgaa gttgtggggt ttcaacaatg atcgcaaccg aggcggagtc
    46741 cgagagaatc caatcaactt gctcaggaga tgaagtttca taaattggaa ctgttactgc
    46801 gccagcaaac cagatagcaa aatctagaac ggtccattca taacgagtgc gagccataat
    46861 tgctacgcga tcgcctaatc cgattccaga tgcaataaga cctttagctg tggcgcgaat
    46921 ctctgcttca aattcacgag cgcttaccgg ctgccaacca tcaccgagtg gacgcgagac
    46981 caagattcgc tctggttcaa accaggctct ctcagctact aagttggtta ggtttccttg
    47041 ggttgctttt ggctctaggg ccggaatgaa gtattcgctc atgggggcaa aattagcggt
    47101 tactggtagg taacgggaag aggtagcctc tgcccatggc cgatatctca tctcactcaa
    47161 cccaaatcca ggctccaatc gagcaggtac gcgaaattct cttcaatctt gaaggttatc
    47221 ccagctggtc aagtgcaatt aaaactgtcg aggtcttgga gcgtgattct tcatctcgcc
    47281 ctacaaagct aacgctgaaa gttgaagcag gagttctaaa agatcgtcca acgctagttt
    47341 atgactggag caaggctccc aatgaaatct ctttctcact tgaagaggca gatctgatga
    47401 ctcagatgga tggcaagtat gagattaaag ataatggtga tgactcagta actgttacct
    47461 actcacttac taccgcgcta agtatgccag ttcctgattt gatgagacgc aaagtagaga
    47521 agacaactat tgagcaatct ctatctgagt taaagcagaa actcgaaggt taaatgtctc
    47581 tggcacttgg tatcgatgtt ggtggtacca aagttcttgg cggagtcgtt gactcatccg
    47641 gcaaaatagt aaagagcgct cgcaaacaga ctccacctca aggcggtcag gccctaactg
    47701 acacaattgc agagctagca ctcgagttaa tttcagagtt tgatatttca gcagttggcg
    47761 ttagcgctgc aggatttatt tcatcagatc gcaaaacaat gcttgcaact ccaaatattg
    47821 ctgggtggaa tggcgtcaac cttactgagc agctagagaa gaaaattggc agaagagttg
    47881 ttttagaaaa tgatgcaaat agcgcagcat ggggcgagaa gaaatttggt gcgggtagag
    47941 attttcacac cgtaattatg ttaactgtag gaaccggctt aggtggcgga ttaattgtta
    48001 atgatcagct ctatcgcggt gcctatggaa tcggcgcaga gtttggtcac accagagcag
    48061 ttcccaatgg acacctctgc ggctgcggcg ttagaggatg ctttgagcaa tatgcctcag
    48121 gaaatgcact tctgcgtcat gcccgcgaag ctattgctgc ctcaccagat ttggctagaa
    48181 accttttggc tagaggtgat tcaagtattg atgggctaac tggaaaacac ttaagcgatg
    48241 ctgcaagaga tggcgatagc gttgcaattg ctgcctttaa caccaccggc cagtggcttg
    48301 gcgcactatg cgcatccctg gttgcatccc ttgatccaga ggcatttatt atcggcgggg
    48361 gagtaataga tgccggagat attctcttaa atccaacaag gcaagcgatg aatgaattaa
    48421 ttccttttac cggcaagcgt ccagctccgg ttattgtccc tgcggtgtta ggaaatgatg
    48481 cgggactagt tggagttgct gatctagcta gaaactagag ctactcaaag gggtatttaa
    48541 ctccaatttc acttcttatc tgatccatga tctccataat ctcttggcta tcttgatggg
    48601 ttattaaatc tgattctttt gcgccagctc tataaagctt ggcaaattca attgcctgct
    48661 ctcgaagacc atggccttca taatttcttg gataatcaat tacctcacca ctagttttta
    48721 ttagacgcat actcgctgga ttatagaaag tgctatcaat ttctatccga gctttgctac
    48781 caattatctc tgccacacat ggagtcttaa ctaacatggt gcaagataaa tttgctacag
    48841 cgccgcttgg ataatcaaaa aatattgagc aatggccatc gactcccgta tctgtaagtt
    48901 cagcagtaac ttgaatttca ttaggttttc ctagaatgaa atgtgcaagt gagatgggat
    48961 agaccccaag atctagaagc gcgccacctg caagctctgg agcatggaga cgataatgtt
    49021 ttggtagcgg aaggaattgg ccatgatcgg cagagataga gatgatttca ccaagctcgc
    49081 cagctgaaag taattgcctg ataagtcggt agtgtggcaa gaagcgagac cacatcgctt
    49141 caactaagag tagattcttt tgctttgcca gtgaaatcat cctggctgat tgggctgcat
    49201 taacggcaaa tggtttctca cagagaacag ctttgcccgc acttagtgca agcagcgtgt
    49261 ttggctcatg catgggatga ggcgttgcaa cataaatagc atctacatca cttgccacca
    49321 gctcttcata gctgccatag gcgctgccac catattcttt actgaaatct aaagctttat
    49381 ctaaagagcg agatccaaca gcgctaactt tatggccact agtaaaggtc agatctttag
    49441 caaatgcctt tgcaatgcct ccagttccta ggattcccca acgaaaattc tcactcatat
    49501 cgcagatcca tctccatctt catctgcatc tggcttgatt tggtagaaga aagcaaaaat
    49561 tccagcaact agagcaattc ctccaggcca aggaccaagg ccaagcagat ctaaaccaag
    49621 aatctgcact gcaattatgt agatcggccc accaattact ccaaggagag cgcttcgttg
    49681 gatgctagtt agctgcggca actccttatt atctccggga tatttctcaa ttgcatcaat
    49741 ctgctctaat tcatcaagaa aagtagtggg agatgattca tcaagtgaga gccctgaaac
    49801 aatggattca aattgggcat caaggctgcc gctatcactt ctttcaactt cgccgcttaa
    49861 tacatcgttt atgaagactc tgctctcttc gatcaaaata tcaaggtcat aatcaagtgc
    49921 cacgttatga aacgatctct caaagactac ttctctaata tcaactgatg aaacgttatc
    49981 aataattact tcagagttag agggatcaac aacgtgatca ttgattgaat aaccaaccat
    50041 tagaggtaga tcgactaaat atagatcttg cctaactaac gcccagagtt tctgcaggga
    50101 atcaaatgct tttaggggag tccttagata gctatgtcgc gggggattgg gggctgcaac
    50161 atcacttcta cttcctttaa ttgatccaac taaatacttt aagagtggaa caagctttac
    50221 tctaagcctg gtatctttta ttgcagggtt aattaatatc aaaccttcaa tctcagatcc
    50281 tctaatactt gcaagacgaa gtgagagcgc accacccata gagaatccag caacaaagac
    50341 gcgctcatgt ttttgcttta actttgtaaa ttctctatca acttcttcat accattgttg
    50401 ccaagtggtg ttattcatat cttcccaagt agtgccgtgg cctggaagac atggagctgc
    50461 aacgctaaat ccatgatctg caaggccctg cgcccaaggc ttaatcgatg caggagagcc
    50521 gttaaatcca tggatcatca aaactgcaac gggtccgctg ggcagataaa aagcttgcgg
    50581 gttaggaatt acgctcacga cacgcatagt gtcataagag gggcaaaagg ctctaaatta
    50641 gggccatggc gctacggcga aaaaagaata aagttgaagc aatggttgct ggcgacaaga
    50701 tcgcctacgg ccttctcaaa tctttcctgc ttccagtgct gaccctgcta tttaggccaa
    50761 aggtttccgg acttcgattt gttcctgcaa ctggtccagt aattattgct tcaaaccatc
    50821 tctcctttag cgattcaata tttatgccgt tagttattcc aagaaaggta acctttctag
    50881 ccaagagtga atacttcaca tctcctgggc ttaaggggct agttaagaag ttgaccttta
    50941 ttgcacttgg ccaagtctcc gttgatcgag caggaggatc tagaagcgag gcagcacttc
    51001 ttaccggact ctctgttctt gcagagggcg gctgtattgg aatttatcca gaaggaacta
    51061 gatctccaga tggccggcta tatagaggaa gaactggaat cgcaagactt gctatggaaa
    51121 gtggcgctcc tattgtgccg gtagcgatgt ttcacactgc agagatacaa ccaactggaa
    51181 aagtaattcc aaagattatg cgagtaaaga tggtctttgg tgagccgatg tattttccaa
    51241 ccccggataa atctaatgat cctgaggagt taaggaaggc aacagattcc ttgatgaaga
    51301 agctacagga gctctcagga caagaatatg tcgatatcta tgcctcacaa gctaaagaag
    51361 ctctagatga gcagaggaga cgcgagagga aagagagcca ggagtaatta ctcctctcca
    51421 aaaatctctc gacgagtagt aataaatttg caagtatcac aagttgagtc agcgcttgga
    51481 acttcgccgc taattacttg aataaaatct tgtagccgct ctgctaggcc aactggattt
    51541 cttgtaattg gataccaggc gctacttaag ttaaggctaa agccactttc agggttgcct
    51601 tcaggatttt caggagacca gacaagaaga ccaaggactg agatctgctt tggctctcct
    51661 tggaggggat tttctagcgc aaaggcatag gcttccagtt gaggtgagta aaaatcactc
    51721 ttgtcgttat tttttccttg gaatttgcaa tcaattacgc catatgtgcc atcatcaaat
    51781 tccatcagta agtcgtattt tcctcttatg gcatagggag aaggatttga gttataggta
    51841 attggcattg atttaaccca accaccgcga tctaagactt tgcctggctt aatttctggg
    51901 tgtaaatctt ttgacttagc gccattgaag tgtttctcct gcatcgcaga gagctcgcca
    51961 actaatggga aaaatgatgg agccttaact ccatggttgt agttaagcca taggcatcga
    52021 tggcatccat tccaagaaaa agttaaatca ctcgggctta taaactctct agctttgctc
    52081 atggcgtgat tctgccttga ggcactgaca tttcatcgct tagatcgctg aagttaaggt
    52141 aaatattcca agggtgatca ttaccagccc gccgatgcgg cgcatgagaa ctaagcgctg
    52201 tggggaggtg gctagccact gcctaactgt tccagcaatt aagccccagg tgccatcaga
    52261 gaagaaagcc agaatggcaa aagtcgcgcc cataattacc agctgctcgg ttacctgctc
    52321 tgatccctta tcaacaaatt gcggcaagat agcggcaaag aaaacaagcc ctttgggatt
    52381 taacgctcca acccagaagc catctttaat agacctaatg tttgaaggct tgatatcgcc
    52441 ttgattgacc atatcgtcgg catgaatttt ggagtgcttt attgcatcaa ggcctagata
    52501 gatcagatag agcccgccaa gaagttgaac tccaataaaa gctaactcac ttgcctgcag
    52561 aatcggaccc aagccaaatg ccacagcaag tgagagagta aatgcgcctg tgacatttcc
    52621 tgcaactgat gcaatcgcag ttgctctgcc ccaagcaata gctctagcga taatgaagag
    52681 aacacttggc cccggggcca aaataatgat catcgcagca attagatatt ccggatatct
    52741 agctgggaag tcgaggatca tcggagaatt atccaacact tttgcctaga aaaagggata
    52801 aagaaagagg cgcctagcgg gtaagccagg cgcctcaatt aactactcta gatagctaat
    52861 ttactgctga tttatgaaac gctagttcca cgcgatttgg agatattgtg aaatacgcga
    52921 taaccaacga ttgcaaccag agttgcgttg atgattccat taaatgtgaa atcaccactg
    52981 gtccaggaga catcagcaat tcctatgatg attgcggtag caaccacgag caaattagtg
    53041 gaatcagtga aattcacctt tgattcaatc cagatttttg ctcctagaat tccgatcatt
    53101 ccaaagagag caacttgcgc tcctcctagg actccgccag gaatagcgct aagaactgcg
    53161 ccaaattttg gtgacactga caagatgatt gcaccaaagg ctgcaatcca atatgccgcg
    53221 gttgaataaa ctcgagtagc agccattacg ccgatatttt cagcataagt ggtggttcct
    53281 gagccgccgc cagcgcccgc aagggtggta gcaacgccat cagcaaatag ggcattaccc
    53341 atctgatcat caagatcttt gccagtcata tttgataccg ctttaacatg cccaacattt
    53401 tcagcaatca gcaccagaac gactgggatg aagagaatga tggcgctgac tttgaattca
    53461 ggagttgtaa aagttggggc tgcaatccat ttagccgagg agattccatc agtgttgaca
    53521 tctcccatga caaatgccac tagatatccc acaacaactc cgaggaaaat cgaaatacgc
    53581 ccaatgaagc cgcgtgagaa agctgcaaat gatgcgattg caagcagagt caaagttcct
    53641 agaactggtc cacttgccaa gccattttta gcagctggag caaggtttag gccgatgacc
    53701 ataacgattg ttccagtaac tacaggaggc aacatccaat taatccagcc agttccaact
    53761 gcgcgagcaa taagaccgac gagggctaat aagactccgg ttgcaactac gccgccgaga
    53821 gcaactgcca tgctgtcact cttaacagat gcggcaattg gagcaaggaa tgcaaaggat
    53881 gatccaagat aacttggaac tttattttga gtaatgatga ggaagagaat cgttcccacg
    53941 ccggagaaga aaagagtggt ggtagggggt aggcccgtaa gaattggaac taggaaagta
    54001 gctccgaaca ttgctgcaat atgttggaaa ccaacgccaa tggtgcgcgg ccaacttaaa
    54061 cgttcatctg tcgagacgac ctcgccaggg gcaatgcttt ttccatttcc gtgtagcttc
    54121 caagagaaga gactcatcta agaggtcctc atttctagag cgcccagtgg ttctgtaggc
    54181 aatatttaaa atgcgggcga gctctagaaa agaagggtat gcctgatatt tgctactaat
    54241 aagtttgact cgcgcaaacc tgagagtttt ataacgacac cctggggatc tttgcgggtc
    54301 agagttgcga atccgcgcct ggcagggttt actatccact cgatatatcg aatcgatata
    54361 aatagggggt ggggtagatg agatctagag ct
//
LOCUS       DAOROD010000007        52513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_19_length_52513_cov_39.029128, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000007 DAOROD010000000
VERSION     DAOROD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 52513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52513
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_52513_cov_39.029128"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLW53_02720"
     CDS             <1..756
                     /locus_tag="PLW53_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36368.1"
                     /translation="EWVEFVNPENPNEIFKCDLTWLTSYWNCIFGQGCQGIDKDLSDH
                     GCCSDGAYYYDKEDEERVTTAAKRLPRSMWQNFDVARKNGKFNITELGLDKDRKTKKI
                     NKTCIFFNESDFTNEYFGCALHHLALAEDKHFVDTKPDICWQLPLRRSWESRSVGDKK
                     YDVIVIGEYTREAWGEGGADMDWYCSSNSEAHNGAEPVYLSHKTELIKLMNESAYEKL
                     TELCKVRIEAQRNRKVKHLPLFVIHPATKAAKS"
     gene            complement(761..1078)
                     /locus_tag="PLW53_02725"
     CDS             complement(761..1078)
                     /locus_tag="PLW53_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36369.1"
                     /translation="MDSVNLKDAYLRAARERPALGINTGLRGEVSLDEARAELAGSIS
                     RILHNLFQDRYGPLVGSLSEPTLIEILKGFEANLSRTKGDVTLVAQELLDRLLPPTSK
                     KRR"
     gene            1116..3482
                     /locus_tag="PLW53_02730"
     CDS             1116..3482
                     /locus_tag="PLW53_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36370.1"
                     /translation="MKQRAMVIRILALIFSLAFSLLTPVSAPALSFKSIPATKWGHLY
                     ASGKITNITAQPRAESVEVGPPRSKWNIEFVGVPEDAKAAFKYAVDIWASYFESTVPI
                     EIEAHWEPSNINGVLGSARPGDYFNAFDGAPDSDLWYPSILADRLAKKDLAPGKPDIV
                     LRFNSNALWYTAIDGRPGRFSYDLSSTVLHEIGHGLGFLSNAEYDKFFGTGYLVQPTP
                     YDAYVQLSDGRLFTDFCARSVELGKAMTSPLYWAGSQATAANNGVKPKLHSPKPYEEG
                     SSITHLDEALFASSGTNSTMTPVLDSGEVFRTPGNIALAMIEDMLLKPPANKAQSLPA
                     KPIDVRALVGDKYALLTFDSPNCRRIDRVTGYTITISPGGQERSFTQSPAKITGLSNG
                     SSYSFTIKAKNDNGESDAVTSNEVTPQATSSTRTIDKKADVKYLAAGVFKKNQVIAYS
                     DAISGNLKLATLVGNSWKSLIVDGISTAGGRSDRNLAGPVSLCVTGAKASEKLHIFYT
                     DTENKDLRHASFDGKKWRYETLDGDGDDIQNYQESVRRKTASDVSVSNACAVTPAGLQ
                     VFYRDESQGIILGAALTKTGWIYEIVDGDRQSEGRTTGDVGFSLSAVASGKSVYLIYD
                     SVLTLSSNNVATQGEVRLAKRNSIYPEDWSYTTLDGPDNGVAVAGYDVSISNSGNRIA
                     AAWLAASGLRLPNADEVRFLIVNEDDYPSRLSPERHGTPGSPLLIDSKGMVFGCQDRI
                     CSVGAYSSTPKLNNGAILNNVKDSAIVTINKVRYAVTSISKKLVLVKL"
     gene            complement(3486..3584)
                     /locus_tag="PLW53_02735"
     CDS             complement(3486..3584)
                     /locus_tag="PLW53_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HRD36371.1"
                     /translation="MGSVVKKRRKRMAKKKHKKLLKKTRIQRRNKK"
     gene            complement(3628..4437)
                     /gene="proC"
                     /locus_tag="PLW53_02740"
     CDS             complement(3628..4437)
                     /gene="proC"
                     /locus_tag="PLW53_02740"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRD36372.1"
                     /translation="MANTYAYSVGVIGAGVMGEALIAAILKAGVVSSKIAITDKRSER
                     TKELEAKYGCHVATLEEIASKAENIFLVVKPQDLDALLASVGSKFLTTQKVISFVAGK
                     KTALIESFISNPVAVLRVMPNTPMSVGVGASAISKGSSATQADVKVVEELLKASGKTI
                     YVDEKLQDAVTATSGSGPAYFFRFVEAMILAAQELGLAESDAKTLVIQTIAGASEMLK
                     QDGASPKTLRENVTSPNGTTFAALEVFEKEDLVGIVKKAMLAARNRSQELS"
     gene            complement(4470..4542)
                     /locus_tag="PLW53_02745"
     tRNA            complement(4470..4542)
                     /locus_tag="PLW53_02745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4507..4509),aa:Thr,seq:cgt)
     gene            complement(4573..5217)
                     /locus_tag="PLW53_02750"
     CDS             complement(4573..5217)
                     /locus_tag="PLW53_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD36373.1"
                     /translation="MRLDHISYAATHDQLVDVVQRIGSRIGSAFTDGGIHPRFGTRNF
                     TLALKNGHYLEVVCPLDHPAADASAFGRVVSQRANEGGGWLTWVVSTDDLSPIENRLG
                     RQAIDGSRKRPDGSELKWKQLGVLGTLNDSQLPFFIQWMSSDHPSTDGKAVAEIVKIE
                     ISGDEKTIEEWLGSDLSKAFDGVEIQWVDPAVNDGETGLVAVHLATPNGVVRLD"
     gene            complement(5237..6376)
                     /locus_tag="PLW53_02755"
     CDS             complement(5237..6376)
                     /locus_tag="PLW53_02755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005926.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRD36374.1"
                     /translation="MSVFDSLVGQSEVVERLKLAINAAKSGSSSQQMVHSWLFVGPPG
                     SGRSNAAIAFAAALVCSESGCGLCRDCQTVLAGTHIDVERFNPTGLSIKAEEVRELIA
                     RSAWSPSVGSWRIVIIEDADRLTETAANALLKTIEEPETHTLWLLCAPTLSDVSVTIR
                     SRCRHLQLRTPSKASVEEFLLANTSVDSKTAAFAARVSQGHIGRARFIATNQDAQSRR
                     KQILSLAFSLKDIDSCFKAASHLLDIAQEQAESENSERDSKELKQLQDAYQGTGRSLV
                     SGGSKAIKDLEKEQKSAATRSVRDNLDSALLDIATIYRDVLVAQSGSDEILNIDLKAE
                     VAEFASTSTPKRSIERIEALLEARINLGRNSAPLSTLEALFCALK"
     gene            complement(6376..8382)
                     /gene="tmk"
                     /locus_tag="PLW53_02760"
     CDS             complement(6376..8382)
                     /gene="tmk"
                     /locus_tag="PLW53_02760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRD36375.1"
                     /translation="MGNSIPDQLSPAAGIRNNVMAIPAFRKLWNAMAFSSFGDWLGLL
                     ATTALAQQLAGGDYATANFAIAGVFIARLLPSVILGPLAGVIADKFDRKKVMVIGDIF
                     RALLFISIPIVGNYFWLYTATVLIECITLFWSPAKDATMPNLVPKNKLESANQMTLLA
                     SYGSAPFAALTFTLLTVFITGISAAFNLGSNSAVDLALFINALSFLFSAFTVSQMDIP
                     KEHLKKGEVSSSLLASLVDGGKFLSSSKVIRGLIFGLLGAFVAAGAVIGLARTFVGDL
                     AGGDAAYGILFSAVFTGLAIGIALGPKVFAQFSRRRLFGAALSVAGFLLVVLSLLQNL
                     VLAVFVVVLLGLFSGVSWVTGFTMIGMEVSDELRGRVFAFTQSLVRISLVGVLAISPL
                     VAAAIGEHQFNFGEVQLTYNGAAFTMLGGGIIAMLVGLLSYQQMKDRPGVSFWSDVVN
                     AFKGELGSITSPKTDGLFISFEGGEGSGKSTQAKLLKEWFENEGKSVVLSREPGGTDL
                     GKNLRKILLDNDTGNISPRSEALLYAADRAHHVYSLIRPALERGDVVITDRYFDSSIA
                     YQGAGRILSPNEVARINRWATESLFPTLTIILDQPADIGLSRIKSADRLESESIDFHN
                     RVRQEYLQLAAVDPERYLVIDARKPIIEIHQEITSRITELSKKR"
     gene            complement(8396..11122)
                     /gene="topA"
                     /locus_tag="PLW53_02765"
     CDS             complement(8396..11122)
                     /gene="topA"
                     /locus_tag="PLW53_02765"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRD36376.1"
                     /translation="MAKNLKKLVIVESPAKARKIGGYLGDDYIVEASIGHIRDLPQRA
                     ADIPKEVKKFAWSREGVDIENGFAPLYVINPDKRAKVAELKELLKESDELILATDEDR
                     EGEAIAWHLMEILKPKVPVKRMVFNEITKEAILKAVNETRDIDERLVDAQETRRVLDR
                     LFGYRLSPVLWKKVMPRISAGRVQSVATRLIVEKERERMAFITSSWWDLTAQTDQNFT
                     ARLISVDDKRLATTNDFDSNGSLKEKNASSILLLDQGSAEKLTNSLEGKKLTVKSIED
                     SPRTERPKPPFTTSTMQQDAGSRLGWGAQLTMRIAQSLYEGGFITYMRTDSVNLSQTA
                     ISAARSAAQSLYGKDHIPESPRFYEGKTKNAQEAHEAIRPAGDSFKTPGELAPELTRD
                     QLALYDLIWKRTVASQMADAKKMQARVDFDVTTSDGKACVFRANGSVITFPGFLAAYE
                     DVSDEKSDDEGDKRLPPMKIGQDVLATKYLCEGHETKPPARYTEPTLVKKLEELGIGR
                     PSTFASIIQTIQDRGYVVKRGRALVPTFLAFSVTGLLEQHFAKLVDYEFTASMEEDLD
                     RIANGEEDRLNWLTKFFFGHDGQPGLQDLAADLGAIDAQQINTMKMGENIEIRVGRYG
                     AYLQEGIGDDRKFANIPEGLAPDELTLQKAIELLAAPSGERELGVDPSSGLQVIAKSG
                     RFGPYITEVFPEVINEDGKKPRKKKDAPTPKTASLLSTMSLDTITMDDALKLLSLPRV
                     LGTDSETGEEITVQNGRYGPYLKRGTDSRTLTSEDELFSINLEQAVDIYKQPKVRRRG
                     VAKPPLKELGADPETKRAIIIKDGRFGMYVTDGETNATLRRGDTVEAMTIERALELLA
                     GRRAWEIENGGVSKKPKRAKKSAPTLTEKTIKGAGSKRKAKKSASGKSKKDK"
     gene            complement(11128..13407)
                     /locus_tag="PLW53_02770"
     CDS             complement(11128..13407)
                     /locus_tag="PLW53_02770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRD36377.1"
                     /translation="MRALSAANSLVQVTGSNLNLVYLVLGVSLVALAIAWALRSQVLA
                     ASAGTPKMQEIAAAVQEGAAAYLARQFKTLSYFVVIVFVLLFALPGDMDVKIGRSIFF
                     LVGAAFSATVGYQGMWLAVRANVRVAEAARQGSAVNAVQIAFRTGGVVGMLTVGLGLF
                     GASLVVVLYQADAPSVLEGFGFGAAMLAMFMRVGGGIFTKAADVGADLVGKVEKNIPE
                     DDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVAALILGKAGFGDMGLVYPLIVP
                     AIGILTAILGIFLTRLRSSDKSAMSAINRSFYLSALISAALVGLATYTYLPSTFAALS
                     GLDAAFQSEVSINPRTFAFGAVVIGIVLAAAIQMLTGFFTEVGKRPVNDVAASSQTGA
                     ATVILAGVSVGFESAVYSALLIAAGVFGAYLLGGGVIVLSLFAVALAGCGLLTTVGVI
                     VAMDTFGPISDNAQGIAEMSGDVKGEGAKILTSLDAVGNTTKAITKGIAIATAVLAAT
                     ALFGAFTDAIKNSVLEAGKVVADQAAQFQGVLDVADPRNLVGLIIGASVVFLFSGLAI
                     NAVSRAAGAVVMEVRNQFKIHPGIMKGTEKPDYARVVDICTRDSLRELATPGLLAVFA
                     PIAVGFGLGVGALGAYLAGSIGTGTLMAVFLANSGGAWDNAKKFVEDGNYGGKGSDAH
                     AATVIGDTVGDPFKDTAGPAINPLIKVMNLVGLLITPAIVAFALDGNERTSQIIAAIA
                     VAIIIAALIRSRRSSTMIG"
     gene            13567..14190
                     /locus_tag="PLW53_02775"
     CDS             13567..14190
                     /locus_tag="PLW53_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRD36378.1"
                     /translation="MNPLDAKEIVATLGTLGVLATLFIETGLLVGLLLPGDSLLFVAG
                     IAASGTAREIFGNQLSYVQLLIWAPIAATVGSQCGHWLGAKYGRPFFDRPNSRFFNQE
                     RVAQTEHWLMKYGLGKALILSHFIPFVRTLINPLCGIIGIPAKKFFIWNIIGSYIWTV
                     SLITAGYLLGERLEGSVDKYLLPIVALIIALSLAPIAIEFLKDRKKR"
     gene            complement(14192..14725)
                     /gene="pyrE"
                     /locus_tag="PLW53_02780"
     CDS             complement(14192..14725)
                     /gene="pyrE"
                     /locus_tag="PLW53_02780"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRD36379.1"
                     /translation="MSARNELKEEILKKAVVHGKVILSSGKEADYYVDLRRVTLDSTA
                     APLVGEVMLELTKDLDFEAVGGLTLGADPVATAMMHVAAQKGRKIDAFVVRKAEKAHG
                     LQRRIEGPDVKGRKVLAVEDTSTTGGSVLTAVEALKEAGAIVVGVAVIVERGAKDKVE
                     SAGFKYLAAYQLSDLGL"
     gene            complement(14735..15118)
                     /locus_tag="PLW53_02785"
     CDS             complement(14735..15118)
                     /locus_tag="PLW53_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD36380.1"
                     /translation="MTTPESDEELALYVISVAAQLSGLHPQTLRQYDRMGLVSPSRAQ
                     GRGRRYSLRDIALLRNVSRMVQEGINLAGIKRIIELESAMSNMAIEVAKLRIEVDALL
                     ESNPEKALVARRRADLIIYNSPKSL"
     gene            complement(15115..16014)
                     /locus_tag="PLW53_02790"
     CDS             complement(15115..16014)
                     /locus_tag="PLW53_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRD36381.1"
                     /translation="MAAKDLYEKDYYAILGVDKKADAATIKKTYRSLARELHPDKTKG
                     DKKLEDRFKEVSEAYEILSDEKKRSEYDQARQAFQSGAFRQGQQFSGDFSDLFSGGGD
                     IFSQLFTGRRGPRKGQDIETEISISFRDALFGKEVDLRLSGQGSSASAITVRIPAGVN
                     DGSKVRVKGRGGPGEAGPGDLFVLVNVIPHPVFARKGENLHISVPVSFTEAALGADIS
                     VPTIEGDEVKLRLASGTQNGKTLRVKGRGVKKGVNAGDLMVTIEVQVPQRIDGKAKKA
                     LEDFANATKDEDLRSEFMQRARA"
     gene            complement(16017..16490)
                     /gene="grpE"
                     /locus_tag="PLW53_02795"
     CDS             complement(16017..16490)
                     /gene="grpE"
                     /locus_tag="PLW53_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRD36382.1"
                     /translation="MSEEIKTEEEVVSTEAVAEVIDPVTALTDDLQRLQAEFANYRKR
                     IERDRALAGEVAISSVLFEFLAILDDLDRAESHGELSGGFKAIAEQITAATTKLGLEK
                     YGSAPEAFDPNIHEALLHETSSEVKETTVTKILQPGYKYKERVLRPARVVVTDPE"
     gene            complement(16490..18313)
                     /gene="dnaK"
                     /locus_tag="PLW53_02800"
     CDS             complement(16490..18313)
                     /gene="dnaK"
                     /locus_tag="PLW53_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRD36383.1"
                     /translation="MARAVGIDLGTTNSCVSVLEGGEPTVIANAEGARTTPSIVAFAK
                     NGEVLVGEVAKRQAVTNVDRTIRSVKRHMGTDWNTSIDGKKYTAQEISARILQKLKRD
                     AEAYLGDTVTDAVITVPAYFNDAQRQATKEAGEIAGLNVLRIINEPTSAALAYGLDKG
                     DKEQTILVFDLGGGTFDVSLLEIGDGVVEVKATNGDNNLGGDDWDQALVDFLVKTFAA
                     KNGVDLAKDKMAMQRVREAAEKAKIELSSSQQTSVNLPYITVDAEKNPLFLDETITRA
                     QFQAMTADLLDRCRRPFQSVLKDAGIPIANIHHVVLVGGSTRMPAVVDLVKELTGGKE
                     PNKGVNPDEVVAVGASLQAGVIKGEVKDVLLLDVTPLSLGIETKGGIMTKLIERNTTI
                     PTKRSEIFTTADDNQPAVQIQVFQGEREMAAYNKRLGMFELTGIAPAPRGIPQIEVTF
                     DIDANGIVHVSAKDLGTGKAQSMTISGGSALPKDEIERMMRDAESHADEDKQRRDEAE
                     TRNTGDSLVYQTEKFLKDNAEKLNEGEAATKKGEVESAMAELKKLLEGADYASIKSAT
                     TKLSEVSQQLGAALYAANASNAEAPKGDDGVEDAEIIDESK"
     gene            18549..19301
                     /locus_tag="PLW53_02805"
     CDS             18549..19301
                     /locus_tag="PLW53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD36384.1"
                     /translation="MNSTVLVKKEPSMTTTNEVKVQKVLVVDDEKSISELITTSLRFV
                     GFEVRTAASGAEALRVAEEFKPHALILDVMLPDLDGFEVCKKLRADGQDVGVLFLTAK
                     DTTEDKIKGLTLGGDDYVTKPFSLEELVARLRALLRRTGVIETSINEELISFADLELN
                     EATHEVRRAGNLIDLSPTEFILLRYLMINADRVVSKSQILDHVWQYDFRGDAGIVETY
                     ISYLRKKIDQTEPPLIHTVRGVGYRLRLPAKN"
     gene            19305..20786
                     /locus_tag="PLW53_02810"
     CDS             19305..20786
                     /locus_tag="PLW53_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD36385.1"
                     /translation="MNQPFRKYKPRPGQWSLRNRLVIGVVALGAFAIAASDFAAQAAF
                     RTFLIAQVDVQLESVAGGSLLRLDRAGIAAEPEETNTKEPLFQPFEPLSDVPSEVSVT
                     LLDASGNILGTVGDIQSPQRIQTLVAGITPDEVSQYNDKPFTLDAGGRGSDYRVLARI
                     LPSSLGSVICAISLDGVEKAMNQLRFLFLAVGFIVLIFLALIARRIIDISLRPLKSVE
                     ETAEAFAKGDFSARLDEARTDTEVGRLTSALNQMLARIEEAFVVKVKSEDKLRRFVAD
                     ASHELRTPLTAIRGFSELHRQGAIKGEEDTSELIRRIEDESVRMSSLVEDLLLLARLD
                     QSPEMLMEPVDIGKLIKEAVESAKAARSTHEISIELESEDLFLLGDSIRIHQVIANLL
                     ANAQIHTPGGSKIIVSAGQNDLATYISVSDNGPGISVESQERIFERFYRADLSRKRSG
                     TEGTGLGLSIVDAVMKAHGGQVKVESELGRGAKFTLTFPIKDL"
     gene            20811..21716
                     /gene="cysD"
                     /locus_tag="PLW53_02815"
     CDS             20811..21716
                     /gene="cysD"
                     /locus_tag="PLW53_02815"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRD36386.1"
                     /translation="MRSSPEILESLENESIEIIRETAASFRNPVFLYSIGKDSSVVLH
                     LIRKAFFPAQVPFPMLHIDTTWKFREMIEFRDQMADKYQIKLMTHTNQDGVRDGVTPF
                     STTASEYTRVMKTVALRQALDSYGFDAAIGGSRRDEEKSRAKERLFSVRESGHRWDPR
                     AQRPELWRTYNPRIRPDQSMRVFPISDWTELDIWSYIQLHNIPVNPLYFAKERPVVKR
                     GEQLIMVDDDRYPLINNEKPEMKKIRFRTLGCYPLTAGVESDAITLEQVVAEVMAVKL
                     SERATRLIDGDKEDSMEKKKKEGYF"
     gene            21716..23551
                     /gene="cysC"
                     /locus_tag="PLW53_02820"
     CDS             21716..23551
                     /gene="cysC"
                     /locus_tag="PLW53_02820"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRD36387.1"
                     /translation="MSSLSSKPIVRLLTCGSVDDGKSTLIGRLLVETDSVPHDTVSSA
                     RSTRRAGSIIPAGEIDFSLLTDGLEAEREQGITIDVAYRSMSLLDGRRLIIADAPGHE
                     QYTRNMAVAASRADIALVLVDATRGVRVQTLRHLTICALMGVEKVIVVINKLDASDFD
                     QKIYDNLVQELTPAIKRLAIEDACFIPVSALEGDNVVYKTAKTAWHAGGSLLEEIQNW
                     REKPRKNEHKRMGVQLISRAENFRGLSGTVSQGEFKVGDEVVILPSAKKAKISRLATF
                     DGDIQSAPSGKAITMVLTPEVDATRGDFIEGADNFTTPADRFSANLVWLGEEELIHSR
                     SYYLINGSSMVAATITTIRHRIDINNGEHESARTLAMNEIGLVEIATDSPIALTKYSE
                     NRISGNFVIVDRVSLNTIGAGMVVHTLRRASNITKHDYEINKATRSQQKGQRAKVIWM
                     TGLSGSGKSTIANALEKELFTKGIHSYVLDGDNLRLGLNKDLGFTKEDRAENVRRVAE
                     VGKLMVDAGLVVIVALVSPFKVDRDHAREIFDSGEFIEVWVKTPAEICAQRDPKGLYK
                     KAREGNLPNLTGVGQDYEAPTSAELILDGTTDIALNVETLMKEVL"
     gene            23548..24303
                     /locus_tag="PLW53_02825"
     CDS             23548..24303
                     /locus_tag="PLW53_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD36388.1"
                     /translation="MNWLFFALAGSIAQLIDGALGMGFGLTSSTLLLTLGTSAAVASA
                     AVHAAEIGTTLASGVSHWHRENVDFEILKRLAIPGGIGAFAGATFLSSIDLSGGTIFI
                     STILLLLGFVLLYRNVFQTNIKPNMAELDNPKYLTFLGATGGFIDASGGGGWGPIVTP
                     TLLATTEHEPRKIIGTVSAAEFIVAVCASVGFLANISRLDIDWSAVGGLALGGVLMAP
                     IAAKLVSVVPRRPLGIAVATAIIVINGVRLVTT"
     gene            complement(24289..25194)
                     /locus_tag="PLW53_02830"
     CDS             complement(24289..25194)
                     /locus_tag="PLW53_02830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRD36389.1"
                     /translation="MADFRALESVNKRRTFFLLFFMAVLVWCISYVAITYFGGTGAGV
                     IPLAVLITLISVWGSYFASDKLVLKMTRAKVISYEDNPNLFNVVNEVVIASGLPMPKV
                     AIVNDPAPNAFATGRNPENALIAFTTGLLESMDRDELQGVTAHEMAHVANRDTLVSAV
                     AATTAGIIALISDILMRMLWFGGGRSRSHQNQMPIFLAIIPLILAPIAATLLKSAISR
                     RRESLADATAVSFTRNPAGLRSALEVLARDNTVVEARSSAVAHFYIESPLDASAASKL
                     FATHPPIQERIETLRKMEANPLSSN"
     gene            complement(25199..25753)
                     /locus_tag="PLW53_02835"
     CDS             complement(25199..25753)
                     /locus_tag="PLW53_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRD36390.1"
                     /translation="MDITVIALALVLFLVVFAIIQYNKLIRLNIQVDEGFSQIEVQLK
                     RRADLIPNLVETVKGYATQEREVFERVVQARAASTTASTLPAVAAADGALTNALRGLL
                     AVAEAYPDLKSSTNFLQLQEELATTENKVSFARQFYNDTVRSLNTAVKTIPSSLFVGL
                     AKVGEREFYEVDDPAVRNAPNVKF"
     gene            complement(25791..26369)
                     /gene="dcd"
                     /locus_tag="PLW53_02840"
     CDS             complement(25791..26369)
                     /gene="dcd"
                     /locus_tag="PLW53_02840"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRD36391.1"
                     /translation="MLLSDRDIAAEIKAGRVQVEPFEAKMIQPSSVDVRLDRFFRVFE
                     NHKYEVIDPSLEQSELTREVAVAPDDFFILHPGEFVLASTYEVITLPDDIAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPVKLYPGMKIGQLCLIKLSSAAEH
                     PYGSAVYGSRYQGQRGPTPSKSWLNFHKTEIK"
     gene            26388..26458
                     /locus_tag="PLW53_02845"
     tRNA            26388..26458
                     /locus_tag="PLW53_02845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26420..26422,aa:Gly,seq:ccc)
     gene            26501..28330
                     /locus_tag="PLW53_02850"
     CDS             26501..28330
                     /locus_tag="PLW53_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD36392.1"
                     /translation="MGHYIANLRDIEFCLFDLLDRESILGKGIYSDLDKATAMGMLEE
                     VKRLCEVDLAESFIEGDRKGTDFDKTTGEVKVPASFKKSYRAYVDGGWGLIDVPTELG
                     GTQIPPSIRWAIAEMVLGSNPAVHIYASGFAFAQVAHVLGDERQKKLAKIMVEKEWGA
                     TMMLTEPDAGSDVGAGRSKAVKQADGTWHITGTKRFITSGDADLFENIVHFVLARPEG
                     AGPGTKGLSLFLIPKFMFDFETGALGKRNGVYVTNVEHKMGLNVSSTCEMNLGEKEPA
                     VGYLLGEVHDGIAQMFKIIEFARMMVGTKAISTLSAGYQQALAYAKTRVQGGDMKDMA
                     NKASPRVTVIHHPDVRRSLMTQKAYAEGMRALILYTATIQDEVLLARANGKSEDEIKD
                     LEHLNDLLLPIVKGYGSERAWTLLGTESLQTFGGSGFTQDWPLEQYVRDAKIDTLYEG
                     TTAIQGLDFFFRKIIKDNGRALTKLSKEIGKVAASGGNNAEEKASLGKALEEMQAIIG
                     LLVTHAMASQEKPDEIYKCGLNTSRLLMAVGDVIISWLLLRQADIAASKIGSASGKEK
                     DFYEGKIAASRYFIHNFLPLISATRAIVEREDGEIMTISEGAF"
     gene            28348..29274
                     /locus_tag="PLW53_02855"
     CDS             28348..29274
                     /locus_tag="PLW53_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD36393.1"
                     /translation="MLTKLRGEIYLLSGALLFSFNGIISKIVLVDGLSAWRLTQIRTG
                     GAFVILFAIYFTFRRSELKTTKKELPWLIAFGVVGVALVQAFYFVAIERLYVGVALLI
                     EFTAPIWILLFLRFVLKKHVPNSLWYAILLSFTGLLLITQVWSGISLDKIGLIAALLD
                     AFALAGYFLIGDRLGKTKTSAAISVWGFGVSSALLFFVLPAWQFPVEVFTKDMELLGR
                     FSQYSLPGWVLILWIVVMGTIAPYLLVVSGLKILSASTASIFGMIEPVLAGIFAWWWL
                     SESLSGIQLIGCVVVLLGIFVADKARVKAGGH"
     gene            29271..29768
                     /locus_tag="PLW53_02860"
     CDS             29271..29768
                     /locus_tag="PLW53_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRD36394.1"
                     /translation="MSSEFDAIFAANSEYVKSYKSSGLSGRAAKGLAIVTCMDSRIDP
                     LRIVGMSAGDVKILRNAGARVTDDMLRTLLLATYLLEVKRVLVMPHTDCRMASGTEEQ
                     IHQALFDASGIDTRSIEIRTVTDQVAALKDDVASIKNYPILPKDLEVVGAIFDVKTGE
                     LRKIV"
     gene            complement(29765..31162)
                     /gene="dnaB"
                     /locus_tag="PLW53_02865"
     CDS             complement(29765..31162)
                     /gene="dnaB"
                     /locus_tag="PLW53_02865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRD36395.1"
                     /translation="MSLAEVPNFDERRSSAPEFDRTPPQDLVAEQSVLGGMLLSKDAI
                     GEVVEVIRHRDFYRPAHELIFEVIVDLYGKGEPADAVTVSAELTKRGEITRAGGAPYL
                     HTLISAVPTAANASYYAKIVRERAIARRLVEAGTKIVQLGYAAEGDVDSIVDMAEQEV
                     YQVTERRAGEDFVQLSSLIPTALDQIEKLQSGELAEGIKTGFKDLDALTNGFHPGNMI
                     VIAARPAVGKSTLGLDIARHASITNGDCSVIFSLEMSRSEITMRMLSAEARVALNNIR
                     SGALSDEEWSRLARRMGEINEAPLFIDDSPNLSMMEIRAKARRLKQRHDLKLVVIDYL
                     QLMTSGKRVENRQQEVSEFSRQLKLLAKELNVPVVAISQLNRSPEQRSDKKPLLSDLR
                     ESGSIEQDADLVILLHRDDLYDSQNRSGEADLIVAKHRNGPTKTITVASLLHFAKFGD
                     IAPKYAAPEKFVKDN"
     gene            complement(31454..31897)
                     /gene="rplI"
                     /locus_tag="PLW53_02870"
     CDS             complement(31454..31897)
                     /gene="rplI"
                     /locus_tag="PLW53_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRD36396.1"
                     /translation="MKLILTREVAGLGNPGDVVNVKDGYARNFLLPRGSAIAWSKGGE
                     SQIEGIRKARQNREIRDKDHANEIKAKLEEANLEIKAKVGATGRLFGSVTEKDIALVI
                     KSATGIDVDRHKIKLVKHVKNLGKHSARVSLMTGVAANITINVVE"
     gene            complement(31912..32190)
                     /gene="rpsR"
                     /locus_tag="PLW53_02875"
     CDS             complement(31912..32190)
                     /gene="rpsR"
                     /locus_tag="PLW53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRD36397.1"
                     /translation="MGARSNRTLKQKPKVSPALNKKFKKKPCSFCRDKVTYIDYKDVA
                     TLRKYITDRGKIRARRITGACTQHQRQISDAVKNSREVALLPYTSTAR"
     gene            complement(32247..32741)
                     /locus_tag="PLW53_02880"
     CDS             complement(32247..32741)
                     /locus_tag="PLW53_02880"
                     /inference="COORDINATES: protein motif:HMM:NF005851.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRD36398.1"
                     /translation="MAAGDTQITLVGNLTSDPELRFTPSGAAVAKFTVASTPRYMDRQ
                     TNEWKDGDTLFLNCQIWRQAAENVAESLTRGMRVIVSGRLKQRSYETKEGEKRTVYEV
                     EVDEVGPSLRNATAKVTKTTRQGGNNTSFSAPTESSDDPWSSPAGVSGGGWTSTTADE
                     QPPF"
     gene            complement(32788..33072)
                     /gene="rpsF"
                     /locus_tag="PLW53_02885"
     CDS             complement(32788..33072)
                     /gene="rpsF"
                     /locus_tag="PLW53_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRD36399.1"
                     /translation="MRRYELMVILDPDLEERTVAPSLETYLKIIKESGGRVDKLDIWG
                     KRRMAFEIAKKSEGIYAVVDMHCEPAAIKELDRQLNLNEAVLRTKVVRPE"
     gene            complement(33206..34432)
                     /locus_tag="PLW53_02890"
     CDS             complement(33206..34432)
                     /locus_tag="PLW53_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36400.1"
                     /translation="MLKSKSLQIALLVTLIATLLSFAKFNHCRGTNWVSPDVYIHMCY
                     SDISALYGARQINTDQWPYASADNSLEYPVLTGVVTYLTGLLIDDPNGYRAYFDVNAF
                     LIVLLLFASVFILWRLAPKYTPLFPIAPAVFGSLFINWDIWAVLFALLAIYFFQNRPN
                     LSALFLGVAISIKFYPGIILFAISLLLWSQSKRLALIKYLAITLISWLVINLPVAINS
                     YEGWMRFFKLNLDRDSDLGSIWYAANLLGFYGGNINNLTIIAFLIALGAIALFFFATA
                     PSRSDLANLSTVAFLTLAAFVTISKVYSPQYILWLTPLAVLALSKDSQRFAFWVWQAS
                     EALYHFAIWQYLASYSGAKFGLSEDLYALAILIRIAGLAYFSRALIKAALAERSQSLR
                     NAQKNPLDFLPDSIYG"
     gene            complement(34434..36473)
                     /locus_tag="PLW53_02895"
     CDS             complement(34434..36473)
                     /locus_tag="PLW53_02895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRD36401.1"
                     /translation="MEWLKKPWVKLGIRISGFLFLIGATAFTIAYFTTDIPDPNDYVN
                     SQSTIIQFSDGDEVGRIGAQNRTIVPLAGVPQDLRRAVLAAEDKGFYSQGAFNPLAIL
                     RGAINTALGRGLQGGSTITQQYAKTAFLTPDRTIQRKIIELIVAIKLENQLSKDQIFE
                     NYLNTIYFGRGAYGVETGAKVYFGKSANQLTIPQAAVLAAILRSPGYYDPEYREGNKE
                     RLEARYKYVLNNMVGEGWLERAEADRYLKKVPEIRPRLSTGQLAGNKGHLIEAVKKEL
                     NSLGFTEEELMIGGLIVRTTLEKGPQTAAETAVFKQSPENAPDDLRIGLVSIRPGTGE
                     IVAMFGGKDYLERQLNDATQGITQAGSTFKPFALVAALEQGISLASVWNGDGPKIFDD
                     FLGRPYEVSNYGNTSFGDVTLLKASAVSVNTIYVPLGIKVGVDKVIDAARRAGIPESV
                     AMVPSPSVVLGVASPRVIDVANSFATFAANGIKSKPFLVKEVLGPNKGILYQARIETE
                     QVFDESVMADLNYALGEVVRAGTASSALRGFGRPAAGKTGTSQSNASAWFTGYTPQLA
                     TSIAFYRNDATESLNGIGGLNSVTGGSFPARIWNAYMKAALKGQPKLDFAPPTFIGGV
                     DSFPIPDPVATIAPSDAAAWCSTADLEGAMKDLKEFCTKALKKYLITTPAPKDKP"
     gene            complement(36501..36899)
                     /locus_tag="PLW53_02900"
     CDS             complement(36501..36899)
                     /locus_tag="PLW53_02900"
                     /inference="COORDINATES: protein motif:HMM:NF036456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HRD36402.1"
                     /translation="MWSKRAYVDFTLRKRATIAAIYRGLNTTSDACDAHPYLRMAAKH
                     HGLLTQRDCPMCRRDKVHELQYTFGDQLGQYSGRIKNDSELDEMQSEFGEFRVYVVEV
                     CLGCGWNHLTASFLLGDGQERKPPRKAKTL"
     gene            36940..38229
                     /locus_tag="PLW53_02905"
     CDS             36940..38229
                     /locus_tag="PLW53_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36403.1"
                     /translation="MNFWKVIRKTAVGRLLALRWMGQFTDGVFQSALASFVLFSPERQ
                     PDAKSAAAAFAVVLLPYSLIGPFVGTILDRVSRQRVILFANLFRALTLIFIAGLIRNG
                     ATGIELTIFVLLAFGVNRLILAGLSAGLPLLINLDGVEGRNNLVSINAAAVTGGTVFV
                     VIGGGVGIGIRTLLEGEVSANQADASLIIFASICFLIAALLSLRLKREQLGPMANEIR
                     KQSIAAAYKEMAEGYRFLRKVRDCFSGIAATAVQRGGLTALTLMALLLNRNTFNDPAD
                     PDAGLAGFAFAITVAGLGITLGAIIAPFGVKKYGRHAWIRYSLFASGILPIALALNQS
                     EFFLVATGFFAGMAGQGVKVTNDALVQSKIDDNYRGRVFAVYDVIVNGGIVSGAIIAA
                     LLLPPSGESAVLPLVIALTYFVFAILLLRKSRFNSDF"
     gene            complement(38213..39652)
                     /locus_tag="PLW53_02910"
     CDS             complement(38213..39652)
                     /locus_tag="PLW53_02910"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRD36404.1"
                     /translation="METPLSAASQIAIATLKERAPEAFELAGLFKAAGFRLALVGGTV
                     RDALLNRLGNDLDFTTDAKPEESKKILKKFADDIWDVGIKFGTIGAKRSETTFEVTTY
                     RADSYEVDTRKPEVQFGETIEDDLRRRDFTVNAMAIELTTDNPEFIDPYNGVTDLLKK
                     ILRTPATAEMSFSDDPLRMLRAARFASQLGFAIDQAALSAMGEMKDRLEIVSAERIRD
                     EFIKIILSDNPRLGINILVESGLCDKFLPEISKLRLEVDEHHHHKDVYEHTLTVLEQA
                     IALEHRLGGANLVVRLAALFHDIGKPKTKALVDGGGVTFHHHEVVGARMAKERLKKLR
                     FDHEVVDAVSELTFLHLRFHGYGDGAWSDSAVRRYVRDAGDLLTHLHVLTRADCTTRN
                     KKKAETLARHYDHLEERIAVLMEQEELLKIRPDLDGNEIMKLLNISAGPIVGKAYEYL
                     LELRLEHGPLGKDRAENELMNWWSTRSQS"
     gene            39717..40304
                     /locus_tag="PLW53_02915"
     CDS             39717..40304
                     /locus_tag="PLW53_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD36405.1"
                     /translation="MTPAPSAGGEEIKKKRKRKRSRSKSSKRSHSNSSDNKSPNQGLN
                     KRHKRTPYAKRVDEVSAGGLVINKSRTQGLLIGRLDAKDPNRERLLWSLPKGHIEEGE
                     SAEAAALREVKEETGIESEIDKSLGVIDFWFMAGGKRIHKTVHHFLFKEVGGKIAPQV
                     SEVDDVRWFPLDEIVTKLAYPDEKKLISRSKDLLS"
     gene            40301..41401
                     /locus_tag="PLW53_02920"
     CDS             40301..41401
                     /locus_tag="PLW53_02920"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HRD36406.1"
                     /translation="MKRILLALLLILLPISPAQSSTLVTLTAPTNKLADGRFINNELA
                     ISISPGGSLGKALEITASSNRTWLIDPALIEEITDLADGYIYLDQEGKDIEVPVFDPA
                     ATWLIKLKSLSANNRVVAITYGAPSQAFLDRIAPGELSTYNALSKLRLEALLDREVSA
                     PGESNIEGQPALIAKNAYTALRKSIKITNSVITSKDVEDLRLGLAKTLNPDLSKDNAL
                     LISKSYSAAIKEVNNNLRISPGNYTITTKNYDLPVTVINDFTESVSLDLIITTTNSRV
                     LVENVPRITIDGQSQIQIEVPIEVIASGDTSLRLQLYTPKGELIGLEQRIPLRLAVIS
                     PVTTWLTTGMAIILLLAAIVQSVRRVKSRRGK"
     gene            41398..42987
                     /gene="murJ"
                     /locus_tag="PLW53_02925"
     CDS             41398..42987
                     /gene="murJ"
                     /locus_tag="PLW53_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRD36407.1"
                     /translation="MSEDANLIRSSSVMGLGTIISRATGLIRNLLLVAALGTGLLGDA
                     FNVANTTPNIIYNLLIGGALSAVFVPLIVQSFRQEDGGSAYISRLLSLIASALLFITI
                     AAMLLAPLLVSLYAPTFSGRSRDVTLAFALYCLPQILFYGLYGILGQVANAKEKFGPM
                     MWAPIANNLLVIVLFSYFISVTDEISLETISDSQIALLGLGSTIGIALQAMILIPTLR
                     KSGLKLSFRRDWRGVGLDKALKLASWAFIFVLISQLGFLITVNLATRAAVLAQEQGID
                     YGVGYTPYANAYLVMLLPHSIVTISIVTALLPGLSKLAFDKKLSEVRDQLSKALRLTA
                     ILTIPASLLFFFFAEEIASAIFFGISEQSAEYIGRVLAAMSLGLIPLSINLVLIRGLN
                     AFENTKYQVISNLVINLVALTISLWAFLNLDVADITVGLGAALAISYWVGIVCTYYLL
                     RKYSGPLNIVSLLLFHSKIAMIAFFSCLAISNLQSRLDLEGNLFSLLIVLLSTFALYL
                     ALGRVLKVSEISQVLKVLLRR"
     gene            42997..43938
                     /gene="trxB"
                     /locus_tag="PLW53_02930"
     CDS             42997..43938
                     /gene="trxB"
                     /locus_tag="PLW53_02930"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRD36408.1"
                     /translation="MSVDYEKVHELVIVGSGPAGYTAGVYAARAQLDPVLYEGSVTAG
                     GALMNTTEVENFPGFKDGIMGPDLMDQMRKQASRFGAQLVTDDITEMKLGSDVKVLKD
                     GSGNTIKSRAVILAMGSAYKEIGLKNEKRLSGRGVSWCATCDGFFFKEQVIAVVGGGD
                     SAMEEANFLTKFAKKVVLIHRRDQLRASKIMAERAKANPKIEFLFNTEVIDVLGEDKV
                     TGLKLRNVLTGEESSSDFTGLFVAIGHLPRSELVVGQVKTNSEGYVLVDSGSTRTNLP
                     GVFASGDLVDFTYRQAITAAGSGCQAALDAERFLAGH"
     gene            44011..44334
                     /gene="trxA"
                     /locus_tag="PLW53_02935"
     CDS             44011..44334
                     /gene="trxA"
                     /locus_tag="PLW53_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRD36409.1"
                     /translation="MATEKVTAANFDAMVLKSTTPVLVDFWAEWCGPCRAVGPILEEI
                     SNEYGSKIKIVKLNTDEEGSIAMKYGINSIPTMNVFVGGQVVKSIVGARPKPMILKEL
                     SEYIN"
     gene            44341..45429
                     /locus_tag="PLW53_02940"
     CDS             44341..45429
                     /locus_tag="PLW53_02940"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD36410.1"
                     /translation="MIKPGDHGDVVTDVTNTLHRLGFIARASNTFSDEVGQAVRAFQQ
                     ERGLTVTGLIDETTQLAINEARWKLGDRSLKLTEPAMRGDDVASLQSRLIEMGFNPGR
                     VDGVYGSQCESAVKDFQKSVGAKIDGICGPATVIALMRLLRTVTGGAPALLRDQASRE
                     KRGPALADKVIVLDPSSRSDIANLSFDLAQKIEGRLIALGVSVFLTRGLNTSPSEEER
                     IAFTNNTSADLLISLSFDRYPNEKASGLATYFYGSETHGIHSIVGEKFATIVQRELIA
                     RTDLLNNRTHAKTWPLLRLTKAPAVLLEIGYISNPKDSARLADPSFRDVIAEALVVAI
                     QRMYLSAEQDAKTGTLRLEDLRKAGIRK"
     gene            45471..46739
                     /locus_tag="PLW53_02945"
     CDS             45471..46739
                     /locus_tag="PLW53_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRD36411.1"
                     /translation="MNSSSSDGLERFQTHKPENLQERYAHRAAHMKPSEIRSLFAVAS
                     RPEIVSLAGGMPNLSAIPMDMMAGIVETLIKDHGQEALQYGSGQGHPQLREQICDVMA
                     LEGIKANPNDVLVTTGSQQALDLISRIFIDPGDVVLVEAPSYVGALGTFAQYEASVVH
                     VEMDQNGLIPEALRQAIKTLRYQGRRIKFLYLIPNYQNPAGVLLPADRRTEILDICRS
                     EKIFIVEDNPYGLLGFDRPSPNAMRAEDSENVIYLGSFSKTIVPGFRIGWALVPQSLK
                     EKLVIASESSILCPSNFSQMAIASYLANQPWREQIASFCALYKVRRDAMLSALDAYFP
                     KAATWTKPAGGFYVWVTLPPEIDTKAMVPKAIAAKVAYVPGTAFYADGFGSWSLRLSY
                     CYPTPERITQGVMALSKVVEQEMLSRGITN"
     gene            complement(46732..47598)
                     /locus_tag="PLW53_02950"
     CDS             complement(46732..47598)
                     /locus_tag="PLW53_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRD36412.1"
                     /translation="MSSKKGGLGRNLDSLIPTPINRVGSNETVGERSEIPLTWIKPNP
                     RQPRTVFDNAALEELAASIKEIGILQPPVVRKIGDNQYELIMGERRYRAAKLAGLVNI
                     PVIVRQTSDNELLREALVENIHRSNLNSLEEAAAYNQMLSDFGFTHDELAERLGKSRP
                     VITNTLRLLNLPPSVQKRLAAGTISAGHARALLGLNSADEMERLANKIVSEGLSVRAI
                     EELIALSNPDQKKSKKSKTKTSDGKYAELVERLEDALDTRVTIAGGNTGGKIVIEYAD
                     GQDLQRIVGVIS"
     gene            complement(47598..48434)
                     /locus_tag="PLW53_02955"
     CDS             complement(47598..48434)
                     /locus_tag="PLW53_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD36413.1"
                     /translation="MAKPITTRIFTVANQKGGVGKTTSAVNVAAALAMGGLKTLIIDL
                     DPQGNASTAFGISRDEVNGMYEVIIKDLPMQSAALKIPGYPNLEVIPATQSLANAEVE
                     LVPMVAREMRLKKSLNNYLLDAANKGERFDYVIIDCPPSLGLLTINALVAAQELLIPI
                     QCEYYSLEGLTLLLHTVSLIQEHLNPNIKLTTILLTMFDGRTRLANDVAAEVRKHYSA
                     ELIDIPIPRAVRISEAPSYGQTVMTYDPASNGAEAYGLVAREIARRGALSSNSNSEKG
                     VG"
     gene            complement(48510..49115)
                     /gene="rsmG"
                     /locus_tag="PLW53_02960"
     CDS             complement(48510..49115)
                     /gene="rsmG"
                     /locus_tag="PLW53_02960"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRD36414.1"
                     /translation="MDSRETRAKRYFPDKPEEALAFEKLLATTAIERGLIGPAEGERI
                     WQRHIENCLPVVTLLPMSGAKVADIGSGAGLPGIVVALARPELKVSLIEPLQRRVEFL
                     YEVIEALKLENVEVIRAKAESVKGSYNFVLARAVAPLPRLIESTWHLIAPGGSLLAIK
                     GESAAQEMATTNLKKFKSHQLHEINLDDLPTGRIIEAVKAG"
     gene            complement(49115..49591)
                     /locus_tag="PLW53_02965"
     CDS             complement(49115..49591)
                     /locus_tag="PLW53_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRD36415.1"
                     /translation="MSEEKTVVTSLEEEGDIAADYLEGLLDIADLDGDIEISVENERA
                     ALVIEGGDLAHLVGGDGEVLDALQELTRLAVQTATGERSRLMLDIDGFRADKKSALAK
                     LAEETADEVKSKGKPIKLAPMNAFERKVIHDTIQKIGLTSESEGEDPDRCVVVMPA"
     gene            complement(49634..50548)
                     /gene="yidC"
                     /locus_tag="PLW53_02970"
     CDS             complement(49634..50548)
                     /gene="yidC"
                     /locus_tag="PLW53_02970"
                     /inference="COORDINATES: protein motif:HMM:NF002350.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRD36416.1"
                     /translation="MGILDPLYWVVSGVMVAIHSALSPVFGGASGVTWTLSIMGLVVL
                     IRIILIPLFVKQIKSQRALTALAPEMKKIQQKYKDDRQKQSEELMKLYKTHKTNPMAS
                     CFPILAQAPIFFALFTVLNGIGKNPPQARGVLTSEIAAQMAKAEFFGAPISQTFLGSS
                     NTTVKIVTVFLIFLMSLTTFTTQRQLMVKGMPKLDSSNNMMLQQQKIMLYLFPIIFAV
                     SGVNFPIGVLIYWSTTNFWTWGQQFYVIKRNPTPGSPAWEELQAKKAKKSGQEPEAEN
                     NGGGVAIEEPKPTGQREQPKKKKKKRRK"
     gene            complement(50836..51168)
                     /gene="rnpA"
                     /locus_tag="PLW53_02975"
     CDS             complement(50836..51168)
                     /gene="rnpA"
                     /locus_tag="PLW53_02975"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRD36417.1"
                     /translation="MLAAINRLTSREDFARVTRSPIRSTTKSLVGYLSKEPNLLSPKI
                     GFVVSRAIGGSVIRHRVTRQLRHASKSSLHILPPQSMVVIRATKPEQNVHNEIPRLFT
                     ALNEKAGR"
     gene            complement(51184..51318)
                     /gene="rpmH"
                     /locus_tag="PLW53_02980"
     CDS             complement(51184..51318)
                     /gene="rpmH"
                     /locus_tag="PLW53_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRD36418.1"
                     /translation="MKRTFQPNNRRRAKKHGFRARMATRGGRAVLSARRAKGRARISA
                     "
     gene            51663..>52513
                     /locus_tag="PLW53_02985"
     CDS             51663..>52513
                     /locus_tag="PLW53_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRD36419.1"
                     /translation="MSIAEPGALEAKEIEADLLELWKGIVDSVAKESPQNRAFLTLTK
                     PLGLLKSDGGSNLLLAAPNVFAKDVLESRLKVLLNDALSAKMGEKINIAVTVDESLDA
                     DLSVVEEVDTEVESPRAGTGRQEVQSKSSDQTQLNPRYIFETFVIGASNRFAHAAAVA
                     VAEAPAKAYNPLFIYGESGLGKTHLLHAIGAYAKELYGGVRVRYVSSEEFTNDFINSI
                     RDDKSSNFQRRYRDLDVLLVDDIQFLENKERTQEEFFHTFNTLYNANKQIVISSDRPP
                     KQLTTLE"
BASE COUNT        14375 a        11488 c        11376 g        15274 t
ORIGIN      
        1 gaatgggttg aatttgttaa ccctgaaaac cctaatgaga tattcaaatg tgatctcacc
       61 tggctaactt cttattggaa ctgcattttt ggccagggct gccaaggtat tgataaggat
      121 ctctctgatc atggctgttg cagcgatggc gcttattact atgacaaaga agatgaagag
      181 cgtgttacta ccgctgccaa gcgacttcca agaagtatgt ggcaaaactt tgatgtggcg
      241 agaaaaaatg gcaaatttaa tattacagag ttgggtctag ataaggaccg taaaacaaaa
      301 aagataaata agacctgtat cttctttaat gagtcagatt tcactaatga gtattttggc
      361 tgcgcccttc atcacttagc tctggctgaa gataaacact tcgttgatac taaacctgat
      421 atctgttggc aactaccgct tcgcagatca tgggaatcta gaagtgttgg tgataaaaag
      481 tatgacgtga ttgttattgg tgaatacacc cgggaggcct ggggcgaggg tggagctgat
      541 atggattggt actgctcttc aaatagtgaa gctcataatg gcgctgagcc agtttatctt
      601 tcccataaaa ctgaattaat taagcttatg aatgaatctg cttatgaaaa actaaccgag
      661 ttatgcaaag ttagaattga agcgcaaaga aatagaaagg ttaaacactt acctctattt
      721 gtgatccacc cagcaacaaa ggctgcaaag agttagttct ttaccttcgt ttctttgatg
      781 ttggcggcaa gagacgatct aggagttctt gagcaactag agttacatcg cccttagttc
      841 tagatagatt ggcttcaaat ccctttaaga tttcaataag tgttggctca ctcaatgatc
      901 cgacaagggg cccatatctg tcttgaaaga ggttatgcaa gattctgctt attgatccgg
      961 ctaattcagc tcttgcttca tcaaggctga cctcacctct tagcccagtg ttgatcccaa
     1021 gggcagggcg ctctcttgct gcgcgtagat aggcatcctt aagatttact gagtccataa
     1081 tcccccatcc ttccagacca ctagcatttg tgcttatgaa gcagagggcc atggtaattc
     1141 gcatccttgc tttgattttc tctcttgcct tctcactttt aacgccagtt tcagctccag
     1201 ctcttagctt taaatcaatt cctgctacta agtggggcca cttatatgct tcaggaaaaa
     1261 ttacaaacat tactgcccag ccaagagcag agagcgttga ggttgggcca cctcgctcta
     1321 agtggaatat tgagtttgta ggagtaccag aagatgccaa ggcggcattt aaatatgccg
     1381 ttgatatatg ggcttcatat tttgaatcaa cagttcccat tgaaattgag gcgcattggg
     1441 agccatcaaa tataaatgga gttttgggtt cagctcgtcc tggtgattac ttcaacgcct
     1501 ttgatggagc tccagattct gatctctggt atccatctat tttggcagat cgcttagcaa
     1561 agaaagatct tgctccaggc aagccagata ttgttctccg ctttaattca aatgcgctct
     1621 ggtataccgc aattgatggc aggccaggac gatttagtta tgacctctcc agcactgttt
     1681 tacatgaaat tggccatggc ttgggctttc tatcaaacgc cgaatatgac aagttttttg
     1741 gaactggata tttagttcag ccaactccat acgatgcata cgttcaactt agtgatggtc
     1801 gattatttac tgacttttgt gctcgctcag tagagcttgg taaagctatg acatctccgc
     1861 tttactgggc tggctcacaa gcaactgctg ctaacaatgg tgttaagcct aaattgcatt
     1921 caccaaagcc ttatgaagaa ggctcatcaa ttacacacct tgatgaggcc ctcttcgcat
     1981 cctctggcac taactccacg atgactccag ttcttgactc tggagaggtt tttagaacgc
     2041 caggaaatat tgctctagcg atgattgaag atatgctctt aaaaccccca gcaaataaag
     2101 ctcaaagcct tccggcaaaa ccaattgatg tcagagcttt ggttggcgat aaatatgcgc
     2161 tacttacttt tgattcccct aactgccgcc gaattgatcg agtaacggga tacacaatca
     2221 ctatcagccc tgggggccag gagcgaagct ttactcaaag cccagcaaaa attactgggt
     2281 taagtaatgg aagtagctac tcatttacta ttaaagctaa aaatgacaat ggggaatccg
     2341 acgctgttac tagtaatgaa gtaactcctc aggccacttc tagcacgaga acaattgata
     2401 agaaggctga tgttaaatac ctagctgctg gagtattcaa gaaaaaccag gttattgcct
     2461 actctgacgc aatttctgga aatctcaaac tggcaacatt ggttggaaac tcttggaaga
     2521 gtttgatagt tgacggtatt tcaactgcag gcgggcggtc agatcgaaat ctagctggac
     2581 cagtatcact ctgcgtgact ggagcaaagg caagtgagaa gctacatatt ttctataccg
     2641 atactgaaaa taaagatctg cgccatgcct catttgatgg caagaagtgg cgttatgaaa
     2701 cattagatgg cgatggcgat gatattcaaa attatcaaga gagcgtaaga agaaaaactg
     2761 ccagtgatgt tagtgtttct aacgcctgcg ccgtaacccc tgctggactt caagttttct
     2821 atcgagatga gtctcaagga attattcttg gcgcagccct tactaaaaca ggttggatat
     2881 atgaaattgt tgatggagac cggcagagcg aaggaagaac aaccggagat gttggattct
     2941 cacttagcgc agtagctagt ggtaagagcg tttatttaat ttatgactca gttctaacgc
     3001 tctcctcaaa taacgtcgcc actcaaggtg aagtaagact tgctaagcgc aactccattt
     3061 atccagaaga ttggagctat accactcttg atggtccaga taatggtgtt gcagttgcag
     3121 gctatgatgt ttcgatcagt aactctggaa ataggattgc agcagcttgg cttgctgcta
     3181 gtggtctgcg cctacctaac gctgatgaag ttcgttttct tattgtcaat gaagatgact
     3241 acccaagtcg cttatcacct gaaagacatg gcactcctgg ctcaccgctt ttaattgaca
     3301 gcaaaggaat ggtctttggg tgccaagatc gaatctgctc agtcggggcc tatagttcca
     3361 cgcctaaatt aaacaatggc gccatcctta acaatgttaa agatagcgcc atagtcacaa
     3421 tcaataaggt ccgctacgcg gtgacctcga tttctaaaaa gttagttctt gtaaaactct
     3481 aagaactact tcttatttct gcgctgaata cgagtcttct ttagaagctt tttatgcttc
     3541 ttcttagcca tgcgcttgcg gcgctttttg actactgaac ccatgctctc aactcctttt
     3601 cggtcttagc taaggttatc tgaatttcta tgatagttct tgagatctat tgcgagccgc
     3661 aagcattgct ttcttcacaa ttccaacaag atcctctttt tcaaatactt caagagcagc
     3721 aaaagttgtg ccgttaggac tagttacatt ttcacgaaga gttttaggtg aggctccgtc
     3781 ttgctttagc atctcagatg ccccggcgat agtctgaatg actaatgtct tagcatcact
     3841 ctctgccaac cctagctctt gggctgccaa aatcattgcc tcaacaaatc taaagaaata
     3901 agccggacct gagccgctgg ttgccgttac ggcatcttgt aacttctcat ctacataaat
     3961 tgtttttcct gatgctttta gcaattcttc tacaactttt acatctgctt gtgtcgctga
     4021 acttcccttt gaaatcgccg aagcaccaac accaactgac attggagtat ttggcattac
     4081 tcgaagaaca gccacaggat tggagatgaa actttcaatt aatgctgtct ttttacctgc
     4141 cacaaacgaa atcacctttt gagttgtcaa gaacttagaa ccaactgatg ctaatagcgc
     4201 atctagatct tgaggtttta caactaagaa aatgttctct gcttttgatg caatttcctc
     4261 aagtgtggca acatgacaac cgtacttagc ttctaattcc ttggttcgct cagacctttt
     4321 atcagttatc gcaatttttg agctaacaac acctgctttg aggattgccg caatcaaggc
     4381 ttcgcccata actcctgcgc caataacgcc aactgaatat gcgtaggtat ttgccatgaa
     4441 gcaaggttag cgaaattttc aatcaattga gccgcccatg agaatcgaac tcatgacctt
     4501 cgctttacga gagcgacgct ctaccgactg agctagggcg gcgatgcttt aaagcttcaa
     4561 gctttgtaag aattaatcca agcgtacaac gccattaggc gttgctaggt gaacagctac
     4621 aagtccggtt tctccatcat ttaccgctgg gtcaacccac tgaatttcaa caccatcgaa
     4681 agcttttgat aggtctgagc caagccactc ttcaattgtc ttttcatcgc cagaaatttc
     4741 aatttttaca atctcagcaa ctgccttgcc atctgttgat gggtgatcac tagacatcca
     4801 ttgaatgaaa aatgggagct gagagtcatt aagagttcca agaactccaa gttgcttcca
     4861 ctttaactca cttccatcag ggcgctttct tgaaccatca atggcttgcc ttccaaggcg
     4921 gttttcaatt ggtgaaagat catctgttga aacaacccaa gtaagccagc cgccgccttc
     4981 attagcgcgc tgtgaaacta cgcgaccaaa tgctgaagca tcagctgctg gatgatccaa
     5041 aggacagaca acttcaagat agtgaccatt cttaagagcg agggtgaagt ttcgagttcc
     5101 aaatcttggg tgaattccgc catcagtaaa ggctgaacca attcgagaac caatcctttg
     5161 aactacatct actagttgat catgagttgc tgcataagag atatggtcta ggcgcatgtg
     5221 ccgataatgc cgaaagctac ttcaaagcgc aaaacagagc ctcaagggta gaaagggggg
     5281 ctgagttacg cccaaggttt atccgggcct ctagtaacgc ttcgatccgc tcaatactgc
     5341 gcttaggagt acttgtgctg gcaaattctg cgacctcggc cttcaaatca atatttaaaa
     5401 tctcatcgct gccgctttga gccaccaata catctcggta gatagtggca atatctaaaa
     5461 gagcgctatc taagttatcg cgcactgacc tagttgcagc tgacttctgc tctttctcta
     5521 ggtctttgat tgcttttgag cccccggaga caagtgagcg ccctgttcct tgataggcat
     5581 cttgtaattg ctttagctct ttactatctc gctccgaatt ttcgctctca gcctgttctt
     5641 gagcaatatc taaaagatgc gaagctgctt taaaacagga atcgatatct tttaatgaga
     5701 aagctagaga aagtatctgc tttcttcttg attgagcatc ttgatttgtt gcaataaatc
     5761 gagcgcgtcc gatatgtcct tgggaaactc gagctgcaaa agctgcagtc tttgaatcaa
     5821 cgctcgtatt tgctagaaga aattcttcaa cacttgcctt tgatggagtt cgcagttgaa
     5881 gatgcctaca tctggatcga atagttaccg atacatcact taaagtagga gcgcacaaga
     5941 gccaaagtgt atgagtctct ggttcttcaa tcgtctttaa tagcgcattg gctgcagtct
     6001 cagttaatct atcagcatct tcaataataa caattcgcca agaaccaact gatggtgacc
     6061 aagcagatct tgctatcaat tcccgaacct cttcggcttt tattgataat ccggtgggat
     6121 taaatctctc aacatcaata tgggtgccag caagaactgt ctggcaatct ctacacaaac
     6181 cacaaccact ttcactgcag accagagctg cagcaaaagc tatggcagca tttgatcgac
     6241 cagaaccggg cggcccaaca aagagccagg agtggaccat ttgttgtgaa gaacttccgc
     6301 tctttgcagc gttaattgct aatttaagtc tctcaactac ctcgctctgg cctaccagtg
     6361 aatcaaaaac gctcattatc tcttctttga tagttctgta attcgagatg ttatctcttg
     6421 gtgaatttca ataattggtt ttctggcatc tataactagg tatcgctcag ggtctaccgc
     6481 tgctaactgc agatactctt gtctgactct attgtgaaaa tcaattgatt cactctctaa
     6541 tctatcagcg ctttttattc gcgatagtcc aatatcggct ggttgatcca agataatggt
     6601 tagggttggg aagagagatt ctgttgccca gcgattaata cgagctactt cattaggtga
     6661 caagattcga ccagctcctt gataggcaat agaggagtca aagtatcggt cggtaatgac
     6721 aacatccccg cgctcaagag cggggcgtat tagtgaataa acatggtgag ctctatcggc
     6781 tgcatataaa agcgcctcag atcttgggga gatatttccg gtgtcattat caagaagtat
     6841 ctttcttaaa ttcttaccta aatctgtgcc gcctggctct ctacttagaa ctacagattt
     6901 accttcattt tcaaaccact cttttaataa ttttgcttgc gtagatttac cagaaccctc
     6961 tccaccttca aaagatataa agagtccatc tgtttttgga gaggtaattg agccaagttc
     7021 gcctttgaat gcattaacaa catcgctcca gaatgaaact cctggccgat ctttcatctg
     7081 ttgataggaa agcagcccaa ctagcattgc aataattccc ccaccaagca tggtaaatgc
     7141 tgctccattg taggtaagtt gaacttcacc aaaattgaat tgatgctcgc caattgctgc
     7201 cgcaactaaa ggcgagattg caagaactcc aacaagtgag atgcgtacta gtgattgagt
     7261 gaaggcaaat actcttccgc gcagctcatc agagacttcc atcccaatca tcgtaaatcc
     7321 agtgacccag gaaactcctg aaaataaacc tagaagcact acaacaaaga ctgcaaggac
     7381 taggttttga agcagagaaa gcacaaccaa taaaaatccc gcaacactta gcgctgctcc
     7441 aaacaagcgt cttcttgaaa actgggcgaa gactttaggc ccgagcgcaa tgccaatagc
     7501 aaggcctgtg aaaacagcgc taaataaaat tccataggca gcatcaccac ctgccagatc
     7561 acctacaaag gttctagcta ggccaattac agcgccagct gcaacgaatg caccgagaag
     7621 accaaaaatg aggcctctga ttactttaga agaggataag aattttcctc catcaacaag
     7681 tgatgcaagt agcgaacttg aaacctctcc cttctttaaa tgctctttag gaatatccat
     7741 ctgacttact gtaaatgcgc taaagagaaa agagagtgca tttataaata gcgccaaatc
     7801 aacagctgag tttgaaccaa gattaaatgc agcactaatt ccagtaataa acactgttaa
     7861 tagcgtgaag gtaagggccg caaaaggggc acttccataa gatgcaagaa gcgtcatctg
     7921 attagcgctc tcaagtttat tttttggaac caaattcggc atagtcgcat cttttgcagg
     7981 gctccaaaat agcgttatgc actcaattag aaccgttgcg gtatagagcc agaaataatt
     8041 tcctacaatc ggtattgata taaataatag agctcggaat atgtcgccaa tcaccattac
     8101 ttttttgcga tcaaacttgt cagcaataac tcctgctagt gggccaagaa tcactgaagg
     8161 tagaagtcgg gcgatgaaaa ctccagctat tgcaaagttt gctgttgcat aatcgccgcc
     8221 agctaattgc tgtgctagcg cagttgtggc aagaagacct aaccaatcac caaaggatga
     8281 aaaagccatc gcattccaaa gctttcgaaa cgccggaatg gccataacgt tattgcgaat
     8341 acccgcagct ggcgatagct gatccggaat tgaattgccc atagatgtaa gcctattact
     8401 tatctttttt tgacttaccg ctggctgact tctttgcttt gcgcttagag ccagctcctt
     8461 taatagtttt ctcagtaagg gttggggcgc tcttcttggc ccgcttaggc ttcttgctta
     8521 ctccaccgtt ttcaatctcc caagcgcgtc gtcccgcaag aagttctaaa gctctctcta
     8581 tcgtcatcgc ttcgactgtg tcgcctcggc gcagtgtggc attggtttca ccatctgtta
     8641 catacattcc gaatcgacca tctttaataa tgattgctct ctttgtttca ggatcagcgc
     8701 ccaactcctt aagtggaggc ttagcaacac cgcgacgcct aacctttggt tgtttgtaga
     8761 tatctactgc ctgctctaga ttgattgaga ataactcatc ttcactagtt aaagtccttg
     8821 aatctgttcc acgttttaaa tacggcccat agcgaccatt ttgtacagtg atttcttcac
     8881 ctgtttcgct atcagttcca agaactcttg gcagagataa aagttttaaa gcatcatcca
     8941 tagtgatggt gtctaagctc attgttgaaa gaagtgatgc tgtttttgga gttggcgcat
     9001 ccttcttctt tcttggtttc tttccatctt cattaattac ttcaggaaag acctcggtta
     9061 tgtatgggcc aaagcgtcca gattttgcaa ttacttgaag tccgctactt ggatcaactc
     9121 caagctctcg ctcacctgaa ggagcagcta ataattcgat tgccttttga agagttaact
     9181 catcaggagc tagtccttct gggatgttgg caaactttcg atcatcgccg atgccttctt
     9241 gaagatatgc accatacctg ccaacacgaa tctcaatatt ttcgcccatc ttcatggtgt
     9301 ttatttgctg cgcatcaatt gcgcccagat ctgctgctaa atcttgcaag cccggctggc
     9361 catcatgtcc aaagaagaat ttagttaacc aattgagtct gtcctcctca ccgtttgcaa
     9421 ttcggtctaa atcttcttcc attgatgcgg tgaattcata gtcaactaac tttgcaaagt
     9481 gctgttcaag taggccagtg actgaaaatg caagaaatgt tggcactagc gctctgcctc
     9541 ttttgaccac ataaccgcga tcttgaatag tttgaataat ggaggcaaaa gttgatggcc
     9601 gtccaatgcc gagctcttct agctttttta ccagagtagg ttcggtataa cgagctggcg
     9661 gctttgtttc atggccctca cataagtatt tggttgctaa aacatcctgc ccaatcttca
     9721 tcggtggaag gcgtttatcg ccttcatcat cacttttttc atcgcttaca tcttcataag
     9781 ctgcaagaaa tcctgggaat gtgatgacgc tgccgttagc tctaaataca caagcctttc
     9841 catcagaggt agtcacatca aaatcaactc ttgcttgcat cttcttagca tcagccatct
     9901 gtgatgcaac agtgcgtttc caaattagat catatagagc taactgatca cgagttaatt
     9961 caggagcaag ctcacctggc gtcttaaaag aatctcccgc agggcgaatt gcttcatgag
    10021 cctcttgagc attcttggtt ttaccttcat aaaatcttgg actctctgga atatggtctt
    10081 tgccatagag agattgcgca gcgcttcgcg cagcgctaat tgcagtctgt gaaaggttga
    10141 cagagtctgt tcgcatatag gtaatgaagc cgccttcata aaggctctgc gcaatacgca
    10201 ttgttaattg cgctccccag ccaaggcgtg atccagcatc ttgctgcatt gtggaggtag
    10261 taaagggagg ctttggtcgc tctgttcttg gtgaatcttc aattgattta actgttaact
    10321 tcttgccttc gagagaattt gttagcttct cagcagagcc ttggtcaaga aggagaatgc
    10381 tcgatgcgtt cttctctttt aaggagccat ttgaatcaaa atcattagtt gtggcaaggc
    10441 gcttatcatc tacagaaatt agccgcgctg tgaagttctg atcagtttgc gcagttaaat
    10501 cccaccaaga tgaggtgata aaagccattc tctcgcgctc tttttcaacg attagccttg
    10561 tagcaactga ctgaaccctt cctgctgaaa tccttggcat tactttcttc cagagcactg
    10621 gggagaggcg atatccaaag aggcgatcta atactcttct agtttcttga gcatcaacca
    10681 agcgctcatc gatatctctg gtttcattta ctgcctttaa aattgcttcc ttagtaatct
    10741 cattaaagac cattcgcttc actggaacct ttggtttcaa gatttccatt aagtgccagg
    10801 caattgcttc gccctcacgg tcttcatctg ttgcaaggat taactcatca gattctttga
    10861 gtagctcttt taactcagca acctttgctc tcttatctgg atttattaca tagagaggag
    10921 caaagccatt ttcaatatca acgccttcgc gcgaccaggc aaacttcttt acttcctttg
    10981 ggatatcagc agcgcgctgc ggcagatctc tgatgtggcc aatggaagcc tcgacgatgt
    11041 aatcatcgcc gaggtaccca ccaatttttc tagcctttgc tggtgattca acaatcacca
    11101 gcttctttaa gttttttgcc aaatgattta gccaatcatt gttgaggagc ggcgactacg
    11161 aataagcgct gcaataatga ttgctacagc aatagctgca attatctggc tagttctctc
    11221 attgccatca agtgcaaagg caacaattgc tggagtgata agaagaccaa ctaagttcat
    11281 cactttgata agtgggttaa tggcagggcc tgcagtgtcc ttaaaaggat caccaacagt
    11341 gtcgccaata actgttgcag cgtgagcatc cgagcccttg ccgccgtagt taccatcttc
    11401 aacaaatttc tttgcgttat cccaggctcc accagagtta gcaagaaata ccgccataag
    11461 cgttccagtt ccaatagagc ctgctaaata agctccaagt gcgccaacgc caaggccaaa
    11521 gccaacagca attggggcaa agactgcaag aagacctggg gttgcaagct cgcggagtga
    11581 atcacgggta cagatatcaa ctactcgagc ataatcaggc ttctctgttc ccttcataat
    11641 tccaggatga atcttgaatt gattcctaac ttccatcacc acagctcctg ctgctcgtga
    11701 tactgcgttg atagcaaggc ctgagaaaag aaataccaca gatgcgccga taattaatcc
    11761 aactaggttt ctaggatcag caacatcaag tacgccttgg aactgagctg cctgatctgc
    11821 gacaactttg ccagcctcaa gaactgaatt cttaattgca tcagtaaacg ctccaaagag
    11881 agcagttgct gcaagaacag cagttgcaat tgcaattccc ttagtaattg cctttgtggt
    11941 attaccaaca gcatcaagtg aggtaagaat ctttgcgcct tcacccttta catctccaga
    12001 catctcagca atgccttgag cattatctga aattggtccg aaggtatcca tcgccacaat
    12061 aacgccaacg gttgttagaa gtccgcaacc agcaagggca acagcaaata gcgagagaac
    12121 aattacgcca ccacctaata gatatgctcc aaatacgcca gcagcaatta atagcgctga
    12181 gtaaaccgct gattcaaaac caacagaaac accagcaaga attactgttg ccgctcctgt
    12241 ttgtgaggat gctgctacat catttactgg acgctttcca acttcagtaa agaagccagt
    12301 tagcatctga attgctgcag ctagaacaat tccaataacc actgcgccaa atgcaaaggt
    12361 tctaggatta attgaaacct cagattggaa tgcggcatca aggcctgaaa gggccgcaaa
    12421 agtgcttggt aaataggtat atgtagcaag gccaacaagg gctgctgaaa tgagggctga
    12481 tagatagaag gagcgattaa ttgcgctcat tgcgctctta tctgaactac gcagacgagt
    12541 taggaagatt ccaaggatcg cagtcaagat tccaattgct ggaacgatta gaggatatac
    12601 aaggcccata tctccaaagc cagccttacc aagaatcaga gctgcaacta gcgttactgc
    12661 atatgactca aagagatctg cggccatacc ggcgcagtct ccaacgttat ctccaacgtt
    12721 atctgcaatt gttgcagcat ttcttgggtc atcttctgga atattttttt caactttacc
    12781 aactagatct gctccaacat cggcagcctt agtaaagatt ccaccaccaa cgcgcataaa
    12841 catggcaagc atcgcagcgc caaaaccaaa tccttctaga actgatggcg catctgcctg
    12901 atagagaaca acaacaagtg atgctccaaa taggccaaga cctacggtta gcattccaac
    12961 tactccacca gttctaaatg caatctgtac tgcattgaca gcagatccct gacgagccgc
    13021 ttctgcaact ctgacgtttg ctctaacagc aagccacatt ccctgatatc ccacagtggc
    13081 agaaaatgct gcgccgacta ggaagaagat tgatctacca atctttacat ccatatctcc
    13141 tggcaatgca aatagaagta caaagacaat aactacaaag taggagagag ttttaaattg
    13201 acgggctaga tatgccgcag ctccttcttg caccgctgct gcgatctctt gcatctttgg
    13261 tgttccagca gatgcagcta atacctgtga gcgaagcgcc caggcgatag caagtgctac
    13321 tagagataca cctagaacta gataaacgag attgagattg ctacccgtga cttgcacgag
    13381 tgagttggcc gctgaaaggg ctctcatatt ttctccgttg gattgctcag gtcagcgcca
    13441 cctgtgtcgg cgctgagcac aatggagcgc agtttaggcg caaaaccccc tgctaaacct
    13501 aatttgagcg tgaatgggga ctaataaata aggagaaggc ggaaaatcag cccttagggt
    13561 gaggctgtga atcccttaga tgccaaggaa attgttgcca cccttggaac ccttggggtt
    13621 ctggcaactt tatttattga aactggcctt ctagttgggc tccttcttcc tggagactcc
    13681 ctgctttttg tggcaggaat agcagcatca ggaacagcaa gagaaatatt tggaaaccaa
    13741 cttagttatg tgcaattgct tatctgggct ccaattgctg ccaccgttgg ttcgcaatgt
    13801 ggtcactggc tcggggcaaa gtatggaaga ccattctttg atcgtcctaa ttctaggttt
    13861 tttaatcaag aaagggttgc gcaaactgag cactggctga tgaaatatgg tttaggaaaa
    13921 gctttaattc tctctcattt cattccgttt gtgcgaactt taattaatcc cctctgtgga
    13981 atcatcggta tcccggcaaa gaaatttttc atctggaaca ttattggtag ttatatctgg
    14041 accgtaagct taattactgc aggctactta cttggagagc ggctagaggg ctccgttgat
    14101 aagtatctac ttccaattgt tgcgctaatt attgctttaa gccttgctcc aattgcaatt
    14161 gaatttttaa aagatagaaa gaaaaggtaa tctacagacc aagatcactg agttgatagg
    14221 cggctaaata tttaaagcct gccgattcca ctttatcttt agctccacgt tcaacaatta
    14281 ctgctacacc aacaacaatt gcgccagctt ctttgagcgc ttcaaccgca gtgagaactg
    14341 agccacctgt agttgaagta tcttcgaccg ccaaaacttt tcttccttta acatctgggc
    14401 cttcaatgcg cctttgtaga ccatgagctt tttctgcttt tctcaccaca aaagcatcta
    14461 tttttcgccc cttttgggca gcgacatgca tcatcgctgt ggcaacaggg tctgcgccaa
    14521 gagttaatcc accaactgct tcaaaatcta aatctttagt tagctcaagc atcacttcgc
    14581 caacaagcgg cgcagcagtt gaatcaagtg ttactcgtcg aaggtcgacg tagtaatcag
    14641 cttcttttcc tgaagagaga atgaccttgc catgaactac tgcttttttt aggatttctt
    14701 ctttcagttc attccgggcg ctcacgagat aaacctaaag gctctttggc gaattgtaaa
    14761 taattaggtc agctcggcgc ctggcaacta aagccttctc tgggtttgac tctaaaagtg
    14821 catcaacttc aattctaagt ttcgcaactt ctattgccat atttgacatt gctgattcaa
    14881 gttcaataat ccgcttaatg cccgccaagt taataccttc ttgcaccatt cgtgaaacat
    14941 ttcgaagtag cgcgatatct cgcagagagt agcgacgacc tctaccttga gcccttgatg
    15001 gtgaaactag ccccatccgg tcatactgcc gaagagtttg tgggtgaagc ccagataatt
    15061 gtgcagcaac tgaaattaca tatagcgcta actcctcatc gctctctgga gttgtcatgc
    15121 gcgtgctcgt tgcataaatt cagatcgtag atcttcatct ttagttgcgt ttgcaaaatc
    15181 ctctagagct tttttagctt tgccatcaat tcgctgcgga acttgaactt caattgtcac
    15241 cattaaatct ccagcgttta cacccttctt aactccgcgt cctttgactc taagggtctt
    15301 cccgttctga gtgcctgatg caaggcgaag ctttacttca tcgccttcaa ttgtgggtac
    15361 tgaaatatca gcgcccagcg ctgcttctgt gaaagaaaca gggacgctga tgtgaagatt
    15421 ttcgcccttt cgagcaaata ctgggtgtgg gatgacattt actagtacaa ataaatctcc
    15481 tgggcctgcc tctcccggac ctccacgacc ttttactcga acttttgaac catcattaac
    15541 ccctgcagga attcgaactg taatagcact tgctgaactg ccctgccctg aaaggcgtag
    15601 gtctacctct ttgccaaata aagcatctcg aaacgaaatt gaaatttctg tttctatatc
    15661 ttggcctttt cgcggcccgc gtcgtccggt aaatagctga gagaaaatgt ctccgccccc
    15721 agaaaataaa tcactgaaat ctccagaaaa ttgttggcct tgtctaaaag cgccagattg
    15781 aaatgcctga cgtgcttgat catattcact gcgtttcttt tcatcactta atatttcata
    15841 agcttcgctg acttctttaa atcgatcctc tagcttctta tctcctttag ttttatcagg
    15901 gtgtaattcg cgagccaggc tgcgataggt cttctttatt gtggcagcat cagctttctt
    15961 atcaacgcct aatatggcgt aataatcttt ttcgtagaga tctttagcgg ccatggttat
    16021 tctggatcag tcactactac tcgagccggt cttaagactc gctctttata tttatatcca
    16081 ggctgcaata tctttgtgac ggtcgtttct ttgacttcac ttgaggtctc atgaagaagt
    16141 gcttcatgga tattgggatc aaatgcctct ggagcgctgc catatttttc taaaccaagc
    16201 ttggtggttg ccgcagtgat ttgctctgca attgccttaa atcccccact taattcgcca
    16261 tgggattcag ctcgatcgag atcgtcaaga atcgctaaaa actcaaataa gacgcttgaa
    16321 attgccactt cgccagctag cgctctatca cgctcgattc gcttgcgata gttagcaaac
    16381 tcagcctgaa gtctttgcaa gtcatcagtt aaggcagtaa ctgggtcaat gacctcagct
    16441 actgcctcag ttgatactac ttcttcttca gtctttatct cctcgctcat tacttgcttt
    16501 catcaatgat ctcagcatct tcgacgccat cgtcgccttt tggcgcttcg gcatttgatg
    16561 cattagcagc gtatagagct gctcctaatt gctgacttac ttcagatagc ttggttgttg
    16621 cgctcttgat gctcgcataa tctgcgcctt caagaagttt cttaagctct gccatagcgc
    16681 tttcaacttc gcccttctta gtagcagctt ctccttcatt gagtttctct gcattatctt
    16741 ttaagaactt ctcagtttga tagacaagag agtctcctgt gttgcgagtc tctgcctcat
    16801 cacgacgctg cttatcttca tcagcatgtg attctgcatc tcgcatcatt cgttcaatct
    16861 catccttagg aagagctgaa ccaccagaga tagtcatgct ctgtgctttt ccagttccta
    16921 aatcttttgc cgatacatga acaattccgt tggcatcaat atcgaatgtg acttcgattt
    16981 gcggtatgcc gcgaggcgca ggagcaatgc ctgttagttc aaacattcct aggcgtttgt
    17041 tatacgcagc catctcacgt tcaccttgga agacttgaat ctgaacagct ggttgattgt
    17101 catcagcggt tgtaaagatc tctgagcgct tagttggaat tgtggtgttg cgttcaatta
    17161 acttggtcat aattccaccc ttggtttcaa taccaagtga tagtggagtt acatcaagta
    17221 ggagaacatc tttaacctca cccttgataa cgccggcttg aaggctggcg ccaacggcga
    17281 ctacttcatc aggatttacg cccttatttg gctcttttcc gccagttagc tctttgacta
    17341 gatcaacgac ggcaggcata cgagttgaac caccaacaag aactacatga tgaatattgg
    17401 caattggaat gccggcatcc tttagtactg attggaatgg gcgacggcag cgatcaagaa
    17461 gatctgcagt cattgcttgg aactgcgccc tggtaattgt ttcatctaag aaaagtggat
    17521 tcttctcagc atctactgtg atgtaaggaa gattgactga tgtttgctga gatgaagaga
    17581 gttcaatctt agctttttca gccgcttctc ttacacgctg cattgccatt ttgtcttttg
    17641 ctaagtcaac gccattttta gcggcaaaag ttttaactaa gaagtcaacg agtgcttgat
    17701 cccaatcatc gccgccaagg ttgttatcgc cattggtagc tttaacttca actacgccat
    17761 caccaatttc aaggagtgaa acatcaaatg ttccgccgcc taggtcaaat actaaaatag
    17821 tctgttcttt atcgccttta tctaggccgt atgcaagagc tgcagatgtt ggctcgttga
    17881 tgatacgtag aacgtttaaa cctgcaattt cgccagcttc tttagtggct tggcgctgcg
    17941 catcgttgaa atatgctggc actgtaataa cagcatcagt aacggtgtca cctaagtatg
    18001 cttctgcatc gcgctttaac ttctgcaaga tacgggctga aatttcttgg gcggtgtatt
    18061 tcttgccgtc aatacttgta ttccagtcag ttcccatgtg gcgcttgact gagcggatgg
    18121 tgcgatcaac gttggtaact gcctgacgct tagcgacctc tccaactaat acttcgccat
    18181 tttttgcaaa ggcaacaatg gagggggtag tgcgcgcacc ctcagcattt gcaataacgg
    18241 tgggttcgcc accttcaagt acggatacgc agctattggt cgtacccaga tcgattccga
    18301 ctgctcttgc catcttttta actccttcaa gctatctatt ggattccaag ggggttaccc
    18361 tccagcattg agccctctac ggtcaaaaga ttgagtcgaa gtggctcaac actgcactag
    18421 ccagaaccca gatagccccc tttctattcc cagcgctagg cattaggcaa aactcgcccc
    18481 tatggcccag cctcttctca ggaagttcta aagtgagccc tacaagatgg ccctcgaggt
    18541 gattactaat gaattcaaca gttctagtta agaaagagcc atcgatgacc acaacaaatg
    18601 aagttaaggt ccaaaaggtc ctagttgttg atgatgagaa gagtattagc gaacttataa
    18661 caacaagcct tcgctttgtc ggttttgagg ttcgcactgc ggcaagtggg gctgaggcct
    18721 tgcgcgttgc cgaggaattt aaaccacatg cgctcattct tgatgtgatg cttccagatc
    18781 tcgatggctt tgaagtttgc aagaagttac gcgcagatgg acaagatgtg ggcgttctat
    18841 tcctaactgc aaaagacact actgaagata aaataaaagg attaactctt ggcggcgatg
    18901 actatgtcac aaagccattt agcttagagg aactcgttgc tcgccttaga gcactattgc
    18961 gcagaactgg agttattgaa acttcaatta atgaagagtt aatctccttt gctgatttag
    19021 agcttaatga ggcaacacat gaagtccgta gggctgggaa tttaattgat ctctctccaa
    19081 cagaatttat ccttcttaga tacttgatga tcaatgcaga tcgcgtggtt tcaaaatcac
    19141 agattcttga ccatgtctgg cagtatgact tccgtggtga tgctggaatt gtcgagacct
    19201 atatttcata tcttcgtaag aagattgatc agactgaacc accacttatc cacactgttc
    19261 gcggtgttgg ttatcgcttg aggttgccag caaagaatta attaatgaat caaccctttc
    19321 gcaaatacaa accaaggcca ggtcaatgga gtctgcgaaa taggttagtc atcggcgttg
    19381 tggctcttgg agcatttgct attgcagcat cagattttgc ggctcaagca gcctttagaa
    19441 cctttctaat tgcccaggta gatgttcagc tagaaagcgt tgctggaggc tcacttttaa
    19501 ggctagatcg cgcaggaatt gcagcagagc cagaggagac aaatacaaaa gagcctctct
    19561 tccaaccctt tgaaccatta agtgatgtgc catctgaagt aagcgtgaca ctacttgatg
    19621 catctggaaa cattttagga acagtaggtg atattcaaag tccgcagaga attcaaactt
    19681 tagtagctgg aataactcca gatgaggtaa gccaatataa cgataagcct tttactctag
    19741 atgctggagg caggggaagt gattatcgag tcctagcaag aattcttccc tcaagccttg
    19801 gaagtgtcat ctgcgcaatt tcattagatg gcgtagagaa agcgatgaat caattacgtt
    19861 ttctctttct cgctgtggga tttatcgtgt tgatcttctt agctttaatt gctcgaagaa
    19921 ttattgatat ctctcttcgc ccattgaaat ctgttgaaga aactgcagag gcttttgcta
    19981 aaggtgactt ctccgctcgt cttgatgaag cacgaactga tactgaagtt ggtcgattga
    20041 catcagcttt gaatcagatg cttgctcgaa ttgaagaagc atttgttgtt aaggttaaaa
    20101 gtgaagataa attacgtcgc tttgttgctg atgcctcaca tgaattgcgc actccactaa
    20161 ctgctattag aggtttttct gaacttcacc gccagggagc aattaagggt gaagaagata
    20221 caagtgaact aatccggcga attgaagatg aatcggtgcg tatgtcatct ttagttgaag
    20281 atctattgct tcttgcccgc ctagatcaat caccagagat gctgatggag ccagttgata
    20341 tcggcaagtt gattaaggaa gctgtggagt cagcgaaagc agcaagatct actcatgaga
    20401 tctccatcga gcttgagagt gaagacttat ttctgctggg ggattccatc cgaatccatc
    20461 aagtaatagc taatttgctc gcaaatgccc agattcacac tccaggtgga agcaaaataa
    20521 ttgtgagcgc cggtcaaaat gatttagcga cctatatctc tgtttcagat aacgggcctg
    20581 gaatttcagt tgaatcccag gaaagaatat ttgaacgttt ttatagggca gatctttctc
    20641 gcaagcggag tgggactgag ggaactggac ttggcttatc aatcgttgat gcagtgatga
    20701 aagctcatgg tggccaagtt aaagttgaat ctgaattagg caggggcgcc aaattcactc
    20761 tcactttccc aatcaaggat ttatagagct gtggacaata ggatttccta atgcgcagct
    20821 ctcctgagat tctcgaatct ttagaaaatg agtcgattga aataataaga gagactgcag
    20881 ctagttttag aaatcctgtc tttttatact caattggcaa agactcatcg gtagttcttc
    20941 atttaattag aaaagcattc tttcctgctc aggttccctt cccgatgctt catatcgata
    21001 ccacttggaa attccgcgaa atgattgaat ttagagatca gatggctgat aagtatcaga
    21061 tcaaattaat gactcacaca aaccaagatg gagttagaga cggagtaact cctttttcaa
    21121 ctaccgcatc tgaatacacc agggtgatga agactgttgc gctacgtcaa gctttagatt
    21181 cctatggatt tgatgctgct attggtggct cacgccgcga tgaagagaaa agtagagcaa
    21241 aagagagatt atttagcgtt agagaatcag gtcatcgctg ggatccaaga gcgcaacgac
    21301 cagagctctg gagaacctat aacccaagaa tcaggccaga tcaaagcatg cgagtatttc
    21361 caatttcaga ttggactgaa cttgatatct ggagctacat ccagcttcac aatatcccag
    21421 taaatccact ctatttcgct aaagaacgcc ctgttgtaaa gcgaggcgaa caattaatta
    21481 tggtcgatga tgatcgctat ccattaatta ataatgaaaa gcccgagatg aagaagattc
    21541 gatttagaac tctaggttgc tacccactaa ctgccggcgt tgaatcagat gccatcacac
    21601 ttgagcaggt agtagcagaa gtgatggctg tgaaattaag tgagcgagcc actcgcctga
    21661 ttgatggcga taaggaagat tctatggaga agaaaaagaa agagggatat ttctaatgtc
    21721 ctcactttct tcaaaaccta tagttcgcct ccttacctgt ggcagcgtcg atgacggaaa
    21781 gagcacctta atcgggcgtt tattagttga aaccgattct gttcctcatg acaccgtttc
    21841 atcagctaga agcacaagga gagcaggatc gataattcca gcaggtgaaa ttgattttag
    21901 tttattaaca gatggtcttg aggcagaacg tgaacaagga ataactattg atgtggcata
    21961 tcgctctatg tcgctacttg atggcagacg attgataatt gccgatgctc cgggccatga
    22021 gcagtacaca agaaacatgg ccgttgctgc atcaagggct gatattgcat tggttctagt
    22081 agatgccaca agaggggtaa gggttcaaac tcttcgccac ttaacaatct gcgcactcat
    22141 gggtgttgaa aaagtaatag ttgttatcaa taaattagat gcaagtgact ttgatcagaa
    22201 gatttatgac aatcttgtcc aagaactaac gcctgctata aaacgtttgg ctattgagga
    22261 tgcttgtttt attccagtaa gcgcacttga aggcgataac gtggtttata aaacagcaaa
    22321 aactgcgtgg catgccggtg gatctctctt ggaggagatt caaaattggc gcgaaaaacc
    22381 tagaaaaaat gagcataaaa gaatgggcgt gcaactaatt tcaagagctg aaaacttccg
    22441 tggcctttca ggcacggtga gccaaggaga atttaaagtt ggcgatgagg tagtaatcct
    22501 tccaagtgct aaaaaagcga aaatttctag gcttgccacc tttgatggcg acattcaaag
    22561 cgcaccaagt ggcaaagcaa taactatggt cttgacccca gaggttgatg caactcgcgg
    22621 tgactttatt gaaggtgctg ataatttcac aacccctgct gatcgcttta gcgcaaactt
    22681 ggtctggtta ggcgaagaag agttaattca ctctcgctct tactacctaa ttaacggctc
    22741 ttcaatggtt gcggcaacga taacaactat tcgccaccga atcgacatta ataatggcga
    22801 gcatgagtca gctagaactc ttgcaatgaa tgagattggt ttagttgaga ttgctactga
    22861 ttcccctatt gcacttacta agtactctga gaaccggata tcaggtaatt ttgtaattgt
    22921 agatcgagta tctcttaaca ctatcggtgc tggaatggtt gttcacacac taagaagagc
    22981 atctaatatc actaagcatg attacgagat caacaaagct actcgctctc aacaaaaagg
    23041 tcagagagct aaagtaattt ggatgactgg gctatctggc tctggaaaat caactatcgc
    23101 taatgctctt gagaaagaac tgtttaccaa agggattcat agctatgtct tagatggcga
    23161 taatttaagg cttgggctaa ataaagatct tggtttcaca aaggaagata gggcagaaaa
    23221 tgtccgccgc gttgcagagg ttggcaaact aatggttgat gccggtcttg ttgtaattgt
    23281 tgctttagta agtccattta aagtagaccg agatcatgca cgagagattt ttgattctgg
    23341 tgaatttatt gaagtttggg tgaaaacccc tgctgaaata tgtgctcaac gagatccaaa
    23401 agggctatat aaaaaggcta gagaaggtaa cctgcctaac ctaactggag tgggtcaaga
    23461 ttatgaggcg ccaacatcgg ctgagttaat ccttgatggc actacagata tcgctttaaa
    23521 tgtagagaca ctaatgaaag aagttctgtg aactggctct tttttgcact cgctgggtca
    23581 attgcccaat taatagatgg cgcacttggt atgggatttg gacttaccag ctcaaccctt
    23641 cttctaactc tgggaacatc agcagcagtg gcatcagcag ctgttcacgc agcagagatt
    23701 ggaactactt tggcatctgg tgtttcacat tggcaccgtg aaaacgttga ctttgaaatc
    23761 ctaaaacgcc tagcaattcc tggaggtata ggtgcatttg ctggggcgac attcttatcc
    23821 tctattgatc taagtggcgg aacaatattt atctcaacaa ttcttctcct acttggattt
    23881 gttctcttgt atagaaatgt ttttcaaaca aatataaaac caaatatggc cgaacttgat
    23941 aatcctaaat acctaacatt cctaggggct actggtggtt ttatagatgc atcaggtggc
    24001 ggtggctggg gaccgatagt tacgccaact cttcttgcca ctacagagca tgaaccaaga
    24061 aaaatcattg gaacagttag cgctgctgaa tttatcgttg cagtttgtgc cagcgttggt
    24121 tttcttgcca atatttctcg tcttgatatt gattggtcgg cagttggtgg ccttgcttta
    24181 ggtggcgttc ttatggcgcc aatcgcagca aaattagtct ctgttgttcc tagaagacca
    24241 cttggtatcg cagttgctac tgcaattatt gttattaacg gtgtgcgctt agttactact
    24301 tagcggatta gcttccatct ttcttaaggt ctcaattcgc tcttgaatcg gaggatgggt
    24361 ggcgaataat ttagaagctg ctgaagcatc aagtggagat tcgatataga agtgggcaac
    24421 agcgcttgat cgagcttcaa ctactgtgtt atccctagct aatacttcaa gtgctgatct
    24481 aagccctgct gggtttctgg tgaatgaaac agctgtggca tcagcaagtg attctctgcg
    24541 gcgagatatt gctgacttca atagcgttgc tgcaattgga gcgagaatta aaggaataat
    24601 ggcgagaaat attggcatct gattctgatg gctcctactt cttccgccgc caaaccagag
    24661 catcctcatc aaaatatcag atatcaaggc aattatccct gcagttgttg cagctactgc
    24721 tgaaactaaa gtgtctcgat tagcaacatg agccatttca tgggctgtta caccttgtaa
    24781 ttcatcgcga tccattgact ctagtaatcc agtggtaaaa gcaataagag cattctctgg
    24841 atttcgtcca gttgcaaagg cattaggagc tgggtcgttt acaattgcca ccttaggcat
    24901 aggaaggcca gaggcaatca caacttcatt gactacatta aataaatttg gattatcctc
    24961 atagctaatt actttcgctc tagtcatttt taatactaac ttgtctgaag caaaatatga
    25021 tccccagact gagattagag tgataagaac tgcaagtggt attactccag cgccagttcc
    25081 accaaagtag gtgattgcaa catatgagat acaccagacc aaaactgcca tgaaaaagag
    25141 cagaaagaag gtccttcgtt tatttactga ttcaagcgcg cgaaaatctg ccatttacct
    25201 agaacttaac atttggagcg tttcgaactg ctgggtcatc aacttcataa aactccctct
    25261 ccccgacctt agcaaggcca acaaatagag atgatggaat cgttttaact gcagtattga
    25321 ggcttctaac agtgtcgtta tagaactggc gagcaaagga gactttattt tctgtagttg
    25381 ccagctcttc ttgaagctgg aggaagttag tagatgattt caaatcagga taagcctcag
    25441 caacagctaa taatccacgc agggcgttag ttagagcgcc atctgccgca gcaactgctg
    25501 gaagagtgga tgcagtagtt gatgccgctc ttgcttgcac aactctctca aatacttccc
    25561 gctcctgtgt ggcatatccc tttacagtct ctacaagatt aggaattaga tctgctcttc
    25621 gctttaactg aacttcaatc tgtgaaaagc cttcatcaac ttggatatta aggcgaatca
    25681 atttgttata ttgaataatg gcaaagacaa caaggaagag aactagagct agcgcaatta
    25741 cggttatatc catggcttaa atctacttaa ttttctaaaa ccctgcttgc ctacttaatt
    25801 tcagttttat ggaaattgag ccaagatttg cttggcgttg ggccgcgctg accttgatac
    25861 ctcgagccat aaactgcaga gccataagga tgctcagcgg cagatgagag tttgatgaga
    25921 cataactgac caatcttcat tcctggatat agcttgactg gaaggtttgc cacatttgaa
    25981 agctcaaggg ttatatgacc actaaatcca ggatcgataa agcccgcagt tgagtgggta
    26041 agcaaaccca ggcgccctaa agaggatttt ccttcaagtc ttccagcaat gtcatctgga
    26101 agagtgatta cttcataagt agaagctaga acaaattcac ctggatggag aataaagaag
    26161 tcatcaggag caacagcgac ctcacgagtt agctctgatt gttctagcga tggatcaatc
    26221 acctcatact tatggttttc aaagactcta aagaatctat cgagtcgaac atcaacactt
    26281 gatggctgaa tcatcttggc ctcaaagggc tcaacctgaa ctcgtcccgc ctttatctct
    26341 gctgcaatat cgcgatcact tagtagcatg ggctcaatgc tattgctgcg ggcgtagtga
    26401 aatggcatca cgccagcttc ccaagctggc agcgcgggtt cgattcccgt cgcccgctcg
    26461 tcgtattgcg taagttactc gccagtagca ttattgggcc atgggccatt acatagctaa
    26521 tctgcgagat atcgagttct gcctttttga tctactagat cgtgaatcaa ttctcggaaa
    26581 aggtatctat tcagaccttg ataaggcaac tgctatggga atgctcgagg aagttaaacg
    26641 cctctgtgaa gtggatttag cagaatcatt tattgaagga gatcgcaaag gaacagattt
    26701 tgataaaaca acaggcgaag tcaaggttcc tgcaagtttt aaaaagtctt atcgcgcata
    26761 tgttgatggt ggttggggct taattgatgt tccaactgaa ttaggtggaa cacaaattcc
    26821 gccatcaatt cgttgggcaa ttgctgaaat ggttcttggt tcaaatcctg cagttcatat
    26881 ctatgcctca ggttttgctt ttgctcaagt tgctcatgtt ttaggtgatg agagacagaa
    26941 gaagcttgca aagattatgg ttgagaaaga gtggggagca accatgatgc tcacagagcc
    27001 tgatgccggc tctgatgttg gagcaggaag atcaaaagct gtgaaacaag cagatggcac
    27061 atggcatata actggaacaa agcgatttat tacttcaggt gatgcagatc tatttgaaaa
    27121 cattgtgcac tttgttcttg cgcgtcctga gggcgctggc cctggaacaa aaggtttgtc
    27181 tctcttcttg attcctaaat ttatgtttga ttttgaaact ggagctctcg gaaaacgcaa
    27241 tggtgtttat gtaacaaatg ttgaacataa aatgggcctt aatgtttcat caacttgcga
    27301 gatgaatctt ggagaaaaag agccagcagt tggatatctg cttggagaag ttcatgatgg
    27361 aattgctcag atgtttaaaa tcattgaatt tgcccgcatg atggtcggaa ctaaggcaat
    27421 ttcaacgctt tccgcgggtt atcagcaggc tttagcctat gcaaaaacca gagtccaagg
    27481 cggcgatatg aaagatatgg ccaataaggc aagcccaaga gttactgtta tccatcatcc
    27541 tgatgttaga agatcgctaa tgacgcaaaa ggcttatgcc gaaggaatga gagcgctaat
    27601 tttatacacc gcaacaattc aagatgaagt acttcttgcc agagctaacg gcaaatcaga
    27661 agatgaaatt aaagatttag agcatctaaa tgatctcttg cttccaattg ttaaaggata
    27721 tggatctgag agagcttgga ctctgcttgg aacagagtcg ctacaaacat ttggtggctc
    27781 tggattcacc caagattggc cgctagagca atatgtcaga gatgccaaga ttgataccct
    27841 ctatgaagga acaacagcga ttcaaggttt agatttcttc ttccgtaaga ttattaaaga
    27901 taatggtcgc gcgcttacaa aactttctaa agagatcggc aaagttgcgg caagtggtgg
    27961 caataatgct gaagagaaag catcccttgg aaaagctttg gaggagatgc aagcaattat
    28021 tggtcttctt gttacccacg cgatggcaag ccaagagaaa cctgatgaaa tctataagtg
    28081 cggactaaat acctcacggc tattgatggc tgttggagat gtaatcattt cttggcttct
    28141 gcttcgccaa gcagatatcg cagcttctaa aattggtagc gcttctggaa aagagaaaga
    28201 tttctatgaa ggaaagattg cagcatctag atacttcatt cataacttct tgcccctaat
    28261 tagcgcaaca agagcgatag ttgagcgcga agatggcgag atcatgacaa tttctgaagg
    28321 agccttttag ccctgtaaag tacgcaagtg ctaactaagc ttcgcggtga gatatacctg
    28381 ctatctgggg ctcttctctt ctcctttaac ggcatcattt caaagattgt tctagtcgat
    28441 ggcctctcgg cctggcgcct gactcagata agaaccggcg gggcatttgt aattcttttt
    28501 gctatctact tcacatttag aagatcagaa ctaaaaacca ccaaaaaaga attgccttgg
    28561 ttaattgcat ttggagttgt aggggtagcg cttgttcaag ccttttactt tgtcgctatt
    28621 gaacgacttt atgtgggcgt ggccttatta attgaattca cagctcctat ttggattctt
    28681 ttatttctac gctttgtttt aaagaaacat gttccaaatt ctctctggta tgccatcttg
    28741 ttaagtttta caggactgct tcttatcact caagtttggt ctggtatctc actggataaa
    28801 atcgggctaa ttgcagccct gcttgatgca tttgcactcg ctggatactt tcttattgga
    28861 gatcgcttag gtaaaacaaa gacgagtgct gcaatttctg tttggggttt tggagtttca
    28921 agcgcccttc tcttctttgt attaccagct tggcaatttc cagtagaggt atttactaaa
    28981 gatatggagc tgcttggtcg ttttagccaa tacagcctcc ctggctgggt tttaattctt
    29041 tggatagttg ttatgggaac tattgcgcca tatcttcttg ttgtcagtgg cctcaagatt
    29101 ctctcagcat cgactgcgag tatctttgga atgatagagc cagttctagc aggaatattt
    29161 gcctggtggt ggctaagtga gagtttgagt ggaatccaat tgatcggctg cgtagtcgtg
    29221 ctactcggta tatttgtcgc tgataaagcg cgagtgaagg caggcggtca ttgagtagtg
    29281 aatttgatgc aatatttgcc gcaaatagcg agtatgtaaa gagctataaa tcaagtggtt
    29341 taagcggccg agcagcaaaa ggtttagcca tcgttacctg tatggattca agaatagatc
    29401 cgttaagaat tgttggaatg agcgcagggg atgtaaaaat cctgcgtaac gctggtgcgc
    29461 gtgtaacaga tgacatgctt cgaaccctgc ttcttgctac ctaccttctt gaggtaaaga
    29521 gagtcttagt aatgccacat accgattgcc gtatggcaag tggaacagag gagcagattc
    29581 atcaagcgct ctttgatgca tcaggcattg atactagatc tattgagatt agaactgtta
    29641 cagatcaagt tgctgctcta aaagatgatg tagcaagtat taaaaattat ccaatacttc
    29701 caaaagattt agaagttgtg ggagcaattt ttgatgttaa aactggggag ctaagaaaaa
    29761 tcgtttagtt atctttaaca aacttttcag gggcagcata cttcggtgca atatcgccaa
    29821 attttgcaaa gtgaagaagc gaggccacag taattgtctt tgttggtcca tttctatgtt
    29881 tagcaacaat tagatctgcc tcgccagagc gattctgtga atcatagaga tcatcacggt
    29941 gtagcaagat aacaaggtcg gcatcctgct cgatagatcc tgattcacga agatcagaga
    30001 gaagtggctt cttatcggag cgctgctctg gagaacgatt tagctgtgag atggcaacaa
    30061 caggaacatt aagctctttt gcaagaagct ttagttgacg agaaaactca gagacctctt
    30121 gctgacgatt ttctacgcgc tttcctgagg tcatcagctg gagatagtca ataacaacta
    30181 atttcaaatc atggcgctgt ttaagtcttc tggcttttgc tctaatttcc atcattgaaa
    30241 gattaggtga atcatcaata aatagcggtg cttcattgat ttcgcccatt cttcgagcaa
    30301 ggcgtgacca ctcttcatcg ctaagcgcac ctgagcgaat attgttaaga gcgactctgg
    30361 cctctgctga gagcatgcgc atagtaattt cagatcggct catctcaagt gagaagataa
    30421 ctgagcaatc accatttgta atagatgcat gtcttgcaat atcaagacca agggttgatt
    30481 taccaactgc aggacgagct gcaatgacaa tcatattccc aggatggaaa ccatttgtta
    30541 gagcatctaa atctttaaaa ccagttttga taccttctgc tagctctcct gattgcaact
    30601 tctcaatctg atcaagcgca gttggaatta aagaacttaa ctgaacaaaa tcttccccgg
    30661 ctcgtctctc agttacttga taaacctctt gctcggccat atcaacaatt gaatcaacat
    30721 cgccctctgc ggcataacct aattgaacaa tctttgttcc tgcttcaaca agtcttctag
    30781 cgatggctcg ctctctaaca atctttgcgt aatagctagc attcgctgca gttggaacag
    30841 cagagataag cgtgtgcaga tatggagcgc caccagctct tgtaatctca ccacgctttg
    30901 ttagctcagc cgaaacagtt actgcatctg ctggttcacc tttgccatag agatcaacaa
    30961 ttacttcaaa gattaattca tgtgctggcc gataaaaatc tcgatgtcta attacttcaa
    31021 caacttcacc aattgcatct tttgataaga gcattccgcc aagaactgat tgttcagcaa
    31081 ctaaatcttg tgggggcgtg cgatcaaatt caggcgcact tgatctgcgc tcatcaaaat
    31141 ttgggacctc agccagactc atgtgcgtat cctcttaccc agcactgaca ggggctaatt
    31201 cattgatttt tgctgtgtat tgggttgtgg gtaaaagttc catatctgtg ggtaagtacc
    31261 ccaaatcctg tgtggagctt gtggaggaaa tgtgggtaac ttccaatttc tcagcaaaca
    31321 cgcaggtcag agagctcttg ctctgtgggt aaagatttat ttcaaattct caatagttga
    31381 gatttcaaac tgctttgcaa ttggaaaata aaaaacggcc cgcgcgtggc gagccgtttt
    31441 ctctaacgtt taattattca acaacgttaa tggtgatatt tgctgccacc ccagtcataa
    31501 gtgagaccct agctgaatgc ttaccaaggt tcttcacatg cttaaccagt ttgatcttat
    31561 ggcggtcaac atcaatacca gttgcgctct taattactaa cgcaatgtcc ttctctgtta
    31621 ctgaaccaaa gaggcgacca gttgctccta cctttgcttt gatttcaagg tttgcttctt
    31681 caagctttgc cttgatctca ttggcatgat ctttatcgcg aatctctcgg ttttgacgag
    31741 ctttgcgaat tccttcaatc tggctctcac cgcccttaga ccaagcaatt gctgatcctc
    31801 taggtagaag gaagtttctg gcatagccat ctttaacatt aactacatcg cctggatttc
    31861 caagacctgc aacttcacga gtaaggatta gtttcatttt ttatcctctc cttatcgcgc
    31921 tgttgaggtg tagggaagta gcgctacttc acgactgttt ttaacagcat cagagatctg
    31981 gcgttggtgc tgagtacaag cacctgtaat tcgtcttgca cgaatcttgc cgcgatcagt
    32041 gatgtatttg cgcagggtcg ctacatcttt atagtcgata taggtgacct tatctctgca
    32101 gaaactgcag ggctttttct taaacttctt atttaacgct ggcgaaacct ttggtttctg
    32161 cttcagcgtc cgattggaac gtgctcccat tgtggtgctc cttcttttag ggcccgcctg
    32221 attaagggcg gaatggttgg tttatattaa aacggtggct gttcatcagc ggtggtggat
    32281 gtccatcctc cgccactaac gcctgccggt gatgaccaag gatcatcgct tgattcagtt
    32341 ggtgcgctaa agctggtgtt attaccgcct tggcgtgtgg tctttgtgac cttagcggtt
    32401 gcgtttcgaa gtgatggacc gacttcatcg acctcaactt cataaacggt gcgcttttcg
    32461 ccttctttag tttcatatga acgctgcttt aaacgaccag agacaattac gcgcattccg
    32521 cgagtaagtg actcggcaac attctctgct gcttgacgcc agatctgaca gttaagaaag
    32581 agagtatctc cgtctttcca ttcatttgtc tggcggtcca tataacgtgg tgttgatgcc
    32641 acggtgaact ttgcaactgc agcaccagat ggtgtgaagc gcagttctgg atcgctagtt
    32701 aaattaccaa cgagtgtgat ttgtgtatct ccggctgcca tttcttatcc tctatctact
    32761 ttaagtggtc tctacttatc tgcggattta ttctggacgg acaacttttg tacgaagcac
    32821 tgcttcattc agatttagtt gacggtccag ctctttaatt gctgctggct cacaatgcat
    32881 atcaacaacg gcataaatac cttcgctctt ttttgcgatt tcaaatgcca ttcttctctt
    32941 gccccagata tcaagtttgt ctacgcgacc acctgattct tttatgatct taagatatgt
    33001 ctcaagactt ggtgccactg tgcgctcttc tagatcggga tctaggatca ccattaattc
    33061 atagcgacgc atgcgttaaa cccacctcct tcggactcat tcggtcacgg tctctccgcg
    33121 acaggagggt tacttacatt tccttaaacc ttcttcttta agcccccaga gcgggagccc
    33181 tttaaaaaag gaaaggaggg cgagcctagc cgtagatact gtcagggaga aaatccaacg
    33241 ggtttttctg tgcattacgc aggctttggg atctctctgc cagagcagcc ttaattaggg
    33301 cccttgagaa ataggcaaga cctgcaattc gaatcaaaat agcaagggcg tagagatcct
    33361 cgcttaggcc aaactttgca ccagagtagg aagctaagta ctgccagata gcaaagtgat
    33421 aaagcgcctc acttgcctgc caaacccaga atgcaaagcg ttggctatct ttacttaaag
    33481 ccaacaccgc aagtggagtt agccagagga tgtactgcgg tgaatagacc ttactaatag
    33541 ttacaaaagc agcaagagtt aaaaaggcaa cggtgcttaa attcgccaga tcacttcttg
    33601 atggggcagt tgcaaagaag aagagcgcaa tagctccaag tgctattaaa aaagcaatga
    33661 tggttaaatt atttatattt ccgccgtaaa acccaaggag atttgcggca taccaaattg
    33721 agccaagatc gctatctcga tcaagattta atttaaagaa acgcatccag ccttcatagc
    33781 tattaatagc taccggcaga ttgataacta gccaggagat taaagtgatt gctaaatatt
    33841 taattaacgc taagcgtttt gactggctcc agagaagcaa ggaaatcgca aaaagtatta
    33901 ttcctggata aaatttaatt gaaatagcaa ctcctaggaa gagtgctgaa agattaggtc
    33961 tgttttgaaa gaagtaaatt gcaagaagag caaataaaac ggcccaaata tcccaattga
    34021 taaagagtga gccaaagact gctggcgcaa ttggaaatag cggggtgtat ttaggagcaa
    34081 ggcgccagag gataaaaaca gatgcaaaca acaaaagcac aatcaagaaa gcattcacat
    34141 caaagtaggc gcgatatcca tttggatcat caattaatag accagttaaa taagtgacta
    34201 ctcctgttag tactggatac tccagtgaat tatcagctga tgcataaggc cattgatctg
    34261 tatttatctg tcttgctcca tagagggctg aaatgtctga ataacacata tggatataga
    34321 catctgggct tacccaattg gtgccgcggc agtgattgaa ttttgcaaag gaaagtagtg
    34381 tggcaatgag cgttactagc agagctatct gcaggctctt gctctttaac actttatggt
    34441 ttgtccttag gagcaggggt agtaattaga tatttcttta acgcctttgt gcagaactct
    34501 tttagatcct tcatagctcc ttcaagatct gcagtagaac accatgcggc agcatctgat
    34561 ggcgcaattg ttgctactgg gtctggaatt ggaaatgaat caactccacc aataaatgtg
    34621 ggcggagcaa aatccaattt tggttggcct ttaagagctg ccttcatata tgcgttccag
    34681 attcgagctg gaaaacttcc accagttaca gaatttagcc cgccaattcc atttagtgat
    34741 tcagtggcat catttctata aaatgcaata gatgttgcaa gttgcggggt atatccagta
    34801 aaccaagcag aagcatttga ttgagaagtt ccagtctttc cggcagccgg tctaccaaat
    34861 cctcttaaag ccgaggatgc agttccagct cttactacct cgccaagtgc gtaattaaga
    34921 tctgccataa cgctctcatc gaagacttgt tcagtctcaa tccgagcttg ataaaggatt
    34981 cctttatttg gacctagcac ttctttaact agaaatggtt ttgatttgat gccatttgcc
    35041 gcaaaggtag caaatgagtt agcaacatct ataactcttg ggcttgctac accaagcact
    35101 accgaaggag atggcaccat agcaacgctc tctggtatgc cagctcttct tgcagcatca
    35161 ataactttat ctacacctac tttaatacct agtggaacat atattgtgtt aacagagaca
    35221 gcgcttgctt taagcagtgt gacgtcacca aatgaggtat tgccataatt tgaaacttca
    35281 tatggccgac ctaaaaagtc atcgaagatc tttggtccat ctccattcca aacacttgca
    35341 agggatattc cttgttcaag ggctgcaacg agggcaaatg gtttaaatgt tgatcctgct
    35401 tgggtaattc cttgcgtagc atcgtttaat tgtcgctcta aataatcttt tccgccaaac
    35461 attgccacaa tctcgccagt tccaggcctg attgaaacta gaccaatacg aagatcatct
    35521 ggggcatttt ctggagattg tttaaagact gcagtctctg ccgcagtctg tgggcctttc
    35581 tctagggtgg ttcgaacaat aagtccgcca atcatcaact cttcttcagt aaaacctaga
    35641 gaatttaatt ctttctttac tgcctcaatt aaatgacctt tgtttcctgc taattgtcca
    35701 gtgctaagtc gaggacgaat ttcaggaacc ttctttaaat acctatcggc ttcagctctc
    35761 tcgagccaac cttcaccaac catgttgtta agaacatatt tataccgagc ctctaagcgc
    35821 tccttatttc cctctcgata ctctgggtcg tagtagccag gggagcgcaa tatcgctgct
    35881 agaactgcag cttgcggaat agttagttgg tttgcactct tgccaaaata aacctttgcc
    35941 ccagtctcta cgccatatgc tccgcggcca aaataaatag tgtttaagta attttcaaag
    36001 atttgatctt ttgataattg attctcaagt ttaatagcaa caattagctc aatgattttt
    36061 ctttgaattg ttcgatctgg agttagaaat gcagtctttg catattgctg agtaattgtt
    36121 gaaccacctt gcaagcctct accaagtgcg gtatttatcg cccctcttaa tattgctagt
    36181 gggttaaaag cgccttgcga ataaaaacct ttatcttcgg cagcaagaac tgctcgtctt
    36241 agatcttgag gcacaccagc tagcggaacg atggtgcgat tttgtgcgcc aattcttccc
    36301 acttcatcgc catcagaaaa ttggattatt gtggattgag aatttacata atcatttgga
    36361 tctggaatat ctgttgtgaa gtaagcgata gtaaatgcgg tggctccaat gagaaataaa
    36421 aagccagaga ttcgaattcc cagctttacc caaggttttt tcagccattc catgtgctta
    36481 gcctaaccaa gtgaaagcta ctagagagtc tttgcctttc gtggtggctt gcgttcttga
    36541 ccatctccta aaaggaaaga ggcagttaag tgattccatc cacagcctaa gcagacttca
    36601 acaacataga cgcgaaactc tccaaattca ctctgcatct catctaattc tgaatcattc
    36661 ttaatacgcc ctgagtattg acctagttga tcaccgaagg tgtactgcaa ctcatgaact
    36721 ttatctctgc gacacattgg acaatctcgc tgcgtgagaa gtccatgatg tttggcagcc
    36781 attcttaagt atggatgggc gtcacaagca tcagaggtgg tgtttagccc gcgatagata
    36841 gcggcaatag ttgcccgctt gcgcaaggtg aaatcaacat aggctcgctt agaccacatc
    36901 ggagaaggat aaagctaaaa gattaactac gcttgagatg tgaatttctg gaaagtcata
    36961 cgaaaaacgg ctgtgggaag actgcttgcc cttcgttgga tggggcaatt cacagatggc
    37021 gtatttcaat cagctctagc ctcctttgtt ttattctcac cggagcgaca accagatgca
    37081 aaatctgctg cggcagcctt tgctgttgtc ttacttcctt actctttaat tgggccattt
    37141 gttggaacta ttcttgatcg agtttccaga cagagagtta ttttatttgc taatttattt
    37201 agggcgctca ctttaatctt tattgcaggg ctaatcagaa atggtgcaac aggaattgag
    37261 ttaacaatct ttgtgcttct tgcctttggt gttaatcgct taatccttgc tggactctct
    37321 gcaggtcttc ctttgctaat caatcttgac ggagttgagg ggagaaataa tctagtttca
    37381 attaatgctg ctgcggttac tggcggaaca gtctttgttg taatcggcgg aggagtagga
    37441 attgggattc gcactctcct agaaggggaa gtcagcgcta atcaagcaga tgccagctta
    37501 ataatttttg cctcaatctg ttttctcatc gcagcacttc tatcgcttcg tttaaagaga
    37561 gagcaattag gccctatggc taatgagatt agaaagcaaa gtattgctgc ggcttataaa
    37621 gagatggctg agggctatag attccttcgc aaagtccggg attgtttctc aggaattgca
    37681 gcaaccgctg ttcaacgcgg cggattaaca gctctaactt taatggcgct tttattgaat
    37741 agaaatactt ttaatgaccc agcagatcct gatgccggtc ttgctggctt tgcttttgca
    37801 attacagtgg caggccttgg tataacactc ggtgcaatca ttgccccatt tggagtaaag
    37861 aaatacggaa gacatgcctg gattagatac tcactttttg caagcggaat actcccgatt
    37921 gctctggcgc ttaatcaaag tgaattcttt ttagttgcca ctggtttctt tgctggcatg
    37981 gcggggcaag gcgttaaggt aacaaatgat gctttagtgc aatcaaagat tgatgataat
    38041 tatcgcggcc gagtatttgc cgtttatgac gtaatagtaa atggcgggat tgtctctggc
    38101 gcaataattg ccgcacttct cttgccacca tctggagaat ctgcggtatt gcctttagta
    38161 attgcgctga catactttgt ctttgcaata ttacttctta gaaagtcgcg ctttaactct
    38221 gacttctagt actccaccaa ttcattaact cattctcggc tctatctttt ccaagtgggc
    38281 catgctcaag tcttaactcc aaaagatatt cataggcttt cccaacaata ggaccagcag
    38341 aaatgttaag aagtttcata atttcgttgc catcaaggtc aggtctaatt tttaatagct
    38401 cttcctgctc catcaagacc gcaatgcgct cttctaaatg atcataatgg cgcgctaaag
    38461 tctctgcttt ctttttattt ctagttgtgc agtcagctct agtaagaaca tgaaggtgag
    38521 ttagaagatc gcctgcatct ctgacatatc ttcgcaccgc agaatcactc caagctccat
    38581 cgccatagcc gtgaaaacgt agatgcagaa aggttaattc gctaaccgca tcaactactt
    38641 catgatcaaa acgaagcttt tttaagcgct ctttggccat tcttgcgccc actacttcat
    38701 ggtgatgaaa ggtcactcca ccgccatcaa ctagggcttt ggttttaggt tttccgatgt
    38761 cgtgaaatag agcagcaagt ctgaccacaa gatttgcgcc acctaaacga tgctctagag
    38821 cgatagcttg ctcaagaacg gtcagcgtat gctcataaac atctttatgg tggtgatgtt
    38881 catcgacttc tagccttaac tttgaaattt ctggaaggaa cttatcgcaa agaccacttt
    38941 caaccaggat attaattcct aggcgaggat tgtcagagag gataatcttt ataaactcat
    39001 ctcttatccg ctctgcagag acaatttcta ggcgatcttt catctcgccc attgcactta
    39061 gcgccgcttg atcaattgca aaccctaatt gtgaagcaaa gcgggcagct cttagcattc
    39121 ttagtggatc atcagaaaat gacatctcag cagttgctgg ggtgcgcagg atcttcttaa
    39181 gtaaatcagt tacgccgtta taaggatcaa taaattcagg attatctgtt gtgagctcaa
    39241 tggccatggc attgacagta aaatcgcgtc ggcgcagatc atcttcaatg gtctcgccaa
    39301 attggacttc tggttttctg gtatctactt catagctatc agccctataa gtggttactt
    39361 caaatgtggt ttcactgcgc tttgcaccaa tggtcccaaa cttaattcca acatcccaaa
    39421 tatcatcagc aaactttttt aatattttct tactttcttc aggctttgca tcagttgtga
    39481 aatctaaatc atttccaagg cgattaagaa gagcatctcg cactgttcca cctacaagag
    39541 caagacgaaa accggcagcc ttaaataacc cagctaattc aaaagcttct ggggcgcgct
    39601 ctttaagcgt tgcgattgcg atctggctcg ctgccgatag tggagtttcc attatgtcta
    39661 aggttaccga tgcaggctta ccttccgttc tcggccatga cgagtagtct tgggccatga
    39721 ccccggcacc tagtgcgggc ggcgaagaaa ttaagaaaaa acgaaagcgc aagcgctctc
    39781 gctctaaatc ttcaaaacgc tcacactcaa atagttctga taataaaagc cctaatcagg
    39841 gtttgaataa gcgccataaa cgcacccctt atgcaaagcg cgttgatgaa gtaagcgctg
    39901 gtgggctggt aatcaataaa tctagaacac aaggactact aattggtcgc ctagatgcaa
    39961 aagatccaaa tcgagaacga cttctctggt ccctgcctaa gggccatatc gaagagggtg
    40021 agagcgctga ggctgcagca cttcgtgagg ttaaggaaga aactggaatt gaaagtgaga
    40081 ttgataaatc acttggcgtt attgacttct ggtttatggc tgggggaaag agaattcata
    40141 aaacggttca tcacttctta tttaaagaag taggaggaaa aatagctcct caggttagcg
    40201 aagtagatga tgttcgctgg tttcccctag atgagattgt tacgaagttg gcctatcctg
    40261 atgagaagaa gttaatctct cgctctaagg acttactgtc ttgaagcgaa ttctcctagc
    40321 tttattactt attttgcttc caatatctcc tgctcaaagt tcaactctgg taacactaac
    40381 tgcgccaaca aataaacttg ctgatggccg atttataaat aatgagcttg caatctctat
    40441 ctcacctggt ggttctttag gaaaggcgtt agagataact gcctctagca atagaacttg
    40501 gttaattgat ccagctttga ttgaagagat cactgatttg gcagatggtt atatttatct
    40561 tgatcaagag ggaaaagaca ttgaagttcc tgtctttgat cctgccgcta cttggttaat
    40621 aaaacttaaa tctctaagtg cgaataaccg agttgttgcc atcacttatg gagctccatc
    40681 tcaagccttt ctagatcgaa tagctccagg ggagctttct acctataacg ctctttcaaa
    40741 acttcgccta gaagcacttc ttgatagaga agttagcgcg cctggagaat caaatattga
    40801 aggtcagcct gcgctgattg caaaaaatgc ctatacagcc ttgcgaaaat caatcaaaat
    40861 tactaattca gtaattacaa gtaaagatgt ggaagacctg cgtctgggac ttgcaaagac
    40921 tttaaatcca gatctttcta aagataatgc gctattgatt tctaaaagct atagcgcagc
    40981 tataaaagag gtaaataaca accttcgcat ctcacctggt aactacacca tcacgactaa
    41041 gaattatgat cttccagtta ctgttattaa cgacttcaca gaatctgtaa gtcttgactt
    41101 aattattaca actactaatt ctcgagtatt agtcgagaat gttcctagga ttactattga
    41161 tggacaatct caaattcaaa ttgaagttcc aattgaggtt atcgcatctg gtgatacctc
    41221 ccttcgcctc cagctctaca cacccaaggg tgagctaatt gggctagagc agagaatccc
    41281 acttcgactc gcggtcattt ctccagttac tacctggttg accactggga tggcgataat
    41341 tcttttacta gccgcaattg tgcagagtgt gagacgagtt aagagcagga gaggcaaatg
    41401 agtgaagatg ccaatctaat tagatcttcc tctgttatgg gccttggaac aataatttca
    41461 agggctactg gcttaattag aaatcttctc cttgttgcag ctcttggaac tggattatta
    41521 ggtgatgctt ttaatgttgc aaataccact cctaatatta tttataacct actaataggt
    41581 ggggcgctat cagcagtatt tgttccgtta attgtgcaat cttttagaca agaagatggc
    41641 ggcagcgcat atatcagccg cttactttca ttaattgcat ctgcgctact ctttataaca
    41701 attgcagcga tgttgctagc tcctctattg gtttcactct atgccccgac tttctccggg
    41761 cgctctagag atgtaaccct tgcttttgct ctctattgtc tgccgcagat tctcttctac
    41821 ggcttatatg gaattctggg tcaagttgcc aatgccaaag aaaaatttgg gccaatgatg
    41881 tgggccccaa ttgctaataa tcttctagtt attgttcttt tctcctactt catctcagta
    41941 acagatgaga tctcacttga aacaataagt gattcacaaa tcgctctact tggcctggga
    42001 agcacgattg gtattgcgct gcaagcaatg attcttattc caaccttgag aaaatcaggt
    42061 ttaaagcttt ctttccgtag ggattggcgt ggagtaggac ttgataaggc tctaaaactt
    42121 gctagttggg cttttatatt cgtcctcata tcacaacttg gttttcttat aacagtaaat
    42181 cttgcaacta gagctgcagt tttagcgcaa gagcaaggaa ttgattacgg agttggttat
    42241 accccgtatg caaatgctta cttagttatg ttgctaccgc attcaatagt cactatctca
    42301 attgtcacag ctcttctccc tggtttatcc aagttagcat ttgataagaa gttatctgaa
    42361 gttagagacc aattgagtaa ggcgcttcga cttactgcca tattgaccat ccctgcatct
    42421 cttctcttct tcttctttgc agaggaaatt gctagcgcga tattcttcgg tatttcagaa
    42481 caatctgcag aatatattgg acgagttctt gctgctatgt ctcttggctt aataccgctt
    42541 tcaattaact tggtattaat tagagggtta aatgcctttg aaaatactaa ataccaggtg
    42601 atctctaatt tagttattaa cttagttgct ttaacaatta gcctttgggc tttcttaaat
    42661 ctagatgtcg cagatataac cgtagggctt ggagccgctc ttgcaatcag ctattgggtg
    42721 ggaatcgttt gcacctatta cttactacga aaatattcag gaccactaaa tatcgtctct
    42781 ttactgctct ttcatagcaa aattgccatg attgctttct tttcttgcct agccatatct
    42841 aacttgcaat caaggcttga tttggagggg aatcttttct cattactcat tgttctctta
    42901 agcacatttg ccctttatct agcgctaggg cgagtgttaa aggtttcaga gattagccaa
    42961 gtattaaaag ttctattgcg aaggtagggt gctctagtga gcgttgatta cgagaaagtc
    43021 catgagctag taattgttgg ctctggtcct gctggatata ccgcaggggt ttatgccgct
    43081 cgcgctcaat tagacccagt tctctatgag ggatcagtta ctgctggtgg cgctttaatg
    43141 aacaccactg aggtagaaaa tttcccaggg tttaaggatg gaattatggg gcctgatctt
    43201 atggatcaga tgagaaaaca agcctctcgc tttggagctc aattagttac agatgacatc
    43261 actgaaatga aattaggttc agatgtaaaa gtattaaaag atggttctgg aaacacgatt
    43321 aagtctcgcg ctgtaattct tgctatgggt tctgcatata aagaaattgg tttaaaaaat
    43381 gagaagcgcc tatcagggcg aggtgtttct tggtgtgcca cttgcgatgg attctttttt
    43441 aaggaacaag taattgctgt tgttggaggc ggagattcag cgatggagga agcaaatttc
    43501 ttaactaaat ttgctaagaa agtggttcta attcatcgta gggatcagct tcgagcatca
    43561 aagattatgg cagagcgcgc aaaggcaaac ccaaaaatag aatttttatt taataccgaa
    43621 gtaatagatg tattaggtga agataaagtt accggcctta aacttagaaa tgtattaact
    43681 ggtgaggaaa gcagctctga cttcaccggt ctatttgttg ctatcgggca tcttccaaga
    43741 agtgaattag ttgttggtca agtaaagact aattctgaag gttatgtatt ggttgattca
    43801 ggatctacta gaaccaactt gcctggggtc tttgcctcag gagatctcgt tgactttact
    43861 tatcgccaag caattactgc ggctggctct ggctgtcagg ccgctttaga tgcggagcga
    43921 ttcttggctg ggcactaaag aaaacgaaat actcttaaga aggcgctaaa gttcgtaaat
    43981 taatatcaat tagagagagg caataagaag atggcaaccg agaaagtgac tgcagcaaac
    44041 tttgatgcaa tggtcttaaa aagcacaact ccagttttag ttgatttttg ggcggagtgg
    44101 tgtgggccat gtcgagcagt aggcccaatt ttggaggaga tctccaatga atatggcagc
    44161 aaaattaaga ttgttaagct caatacagat gaagaaggct caattgctat gaaatatggc
    44221 atcaactcaa tcccaacaat gaacgttttt gttggcggac aggttgttaa gagcatcgtt
    44281 ggtgctcgtc ctaagccaat gattctcaaa gagctctctg agtacatcaa ctaaagagca
    44341 atgattaagc ccggcgatca cggggatgtt gtaaccgatg ttacaaacac cctgcatcgc
    44401 cttggcttca tagcaagagc aagcaacaca ttctcagatg aggtaggcca agcagttcgc
    44461 gcttttcaac aagagcgtgg tctgacagtg actggactaa ttgatgaaac aactcagctt
    44521 gcaattaatg aagcgcgttg gaaacttggt gatcgctctc ttaagttaac tgaacctgct
    44581 atgcgcgggg atgatgtggc atcgttacag agccgactaa ttgaaatggg atttaaccca
    44641 ggtagagtcg atggggttta tggaagtcag tgtgagagcg cagtaaaaga ttttcaaaaa
    44701 tcagtgggtg caaaaattga tggaatttgt gggcctgcaa cagtaattgc ccttatgcgc
    44761 ttgctgcgca cagtaactgg aggagcccca gcgctattgc gagatcaagc aagtagagaa
    44821 aagcgtggtc ctgcccttgc agataaggta attgttctag atccaagctc tagatcagat
    44881 attgctaacc tgagttttga tcttgctcaa aaaattgaag gaaggcttat tgcacttggt
    44941 gtctcagtat ttctaactag aggtttaaat acctcaccaa gtgaagaaga gcgaatagct
    45001 tttaccaata acacatcagc tgatctttta atatccttga gttttgatcg atatcccaat
    45061 gagaaagcaa gtgggcttgc cacatacttc tatggcagtg agactcatgg aattcattca
    45121 atagtaggtg agaaatttgc aactattgtg cagcgagaat taattgcaag aactgatcta
    45181 ttaaataata gaacgcatgc taaaacttgg ccgctacttc gattaacaaa ggctcctgca
    45241 gttttgttag aaattggcta tatctcaaat ccaaaagatt ctgcgcgatt ggcagaccca
    45301 agttttcgcg atgtgatagc tgaggcttta gttgtcgcca ttcaaagaat gtatttatct
    45361 gctgagcaag atgcaaagac tggaactttg cgcctagagg atctacgcaa agctggaata
    45421 agaaagtaaa tctgcgccaa gcaagctaat tgttatggca gacttcccca atgaattcct
    45481 cctcatctga tggtttagag cgattccaaa cgcataaacc agagaacttg caagagcggt
    45541 atgcccaccg ggccgctcat atgaagccaa gtgaaatcag atctttattt gctgttgcat
    45601 ctcggcctga aattgtttca ctcgctggag gaatgccgaa tttaagcgca ataccgatgg
    45661 atatgatggc tggaattgtt gaaacgttaa ttaaagatca tggccaagag gcgctccaat
    45721 atggaagtgg gcaaggacac ccccagcttc gcgagcagat ttgtgatgtg atggctcttg
    45781 aaggaattaa agcaaacccc aatgatgtct tggttactac aggttctcag caagcgctgg
    45841 atttaatttc caggattttt attgatccag gcgatgtggt tttagttgaa gcaccttcat
    45901 acgttggagc tcttggaact tttgcccagt atgaggcaag cgtggttcat gttgagatgg
    45961 atcaaaacgg gcttattcct gaagcgcttc gtcaagcaat taaaaccttg agatatcagg
    46021 ggcgaagaat taagtttcta tatttaattc ctaattatca aaatccggct ggggtattac
    46081 tgcctgcaga tcgaagaact gaaatccttg atatttgtcg ctctgaaaag atctttatcg
    46141 ttgaagacaa tccttatggc ctgcttggtt ttgatcgccc atcaccaaat gcgatgcgag
    46201 ctgaagattc agaaaatgtc atctatctcg gttctttctc caaaacgata gtccctggtt
    46261 ttcgaattgg ctgggctcta gttccgcaat ctttgaagga aaagttggtt atcgcatcag
    46321 aatcctcgat cctctgcccc tcaaactttt ctcaaatggc tatcgctagc tatttagcta
    46381 accagccttg gcgcgaacaa attgcctctt tctgcgcgct ttataaagtt aggcgagatg
    46441 ccatgctctc tgccctcgat gcctattttc caaaggctgc aacatggaca aagcctgctg
    46501 gtggttttta tgtctgggtt accttgccac ctgagataga cacaaaagct atggttccaa
    46561 aggcaattgc agcaaaggtt gcctacgttc cagggacagc tttttatgct gatggttttg
    46621 gatcttggtc gctccgtctt tcttattgtt acccaactcc tgaaagaatt actcaaggag
    46681 ttatggcttt gagtaaagtg gtggagcaag agatgttaag ccgcggaatt actaactaat
    46741 aactcccaca atcctctgta agtcttgacc gtctgcatat tcaataacaa tctttccgcc
    46801 agtattacct ccagcaattg tgaccctggt atctagcgca tcttctaaac gctcaacaag
    46861 ctcggcatac tttccatcac ttgtttttgt tttgctcttc ttggatttct tctgatcagg
    46921 gttacttaaa gcaattagct cctcgatagc tctaacgctc aagccctcac tgacgatttt
    46981 atttgcaagt cgctccattt catcagctga gttaagacca agaagcgctc tggcatgtcc
    47041 tgcgctgata gttccggctg ccaatcgttt ctgaaccgat ggcggcagat ttaaaagcct
    47101 taaggtgtta gtaattaccg ggcgagattt tccaagtcgc tctgcgagtt catcatgggt
    47161 gaatccaaag tcactaagca tctggttata tgccgcagcc tcttctagtg agtttaagtt
    47221 actgcgatgg atattttcta ctaaagcttc gcgaagtaat tcattatcgc tagtttgacg
    47281 aacgatcact ggaatattta ctaaacctgc aagtttggcc gcccgatagc gccgctctcc
    47341 cataattaac tcatattgat tatcaccaat ttttcgcaca actggcggtt gcaggattcc
    47401 tatctcttta attgatgcag caagttcttc caaagctgcg ttatcaaaga cggttcttgg
    47461 ttgccttggg tttggcttaa tccaagttaa tggaatctca cttcgctcac caactgtctc
    47521 attacttccc acacgattaa ttggggttgg aataagtgaa tcaagatttc ttccaaggcc
    47581 accttttttg ctactcatca accaacacct ttctcgctat ttgaattaga tgaaagtgcg
    47641 ccgcgccttg caatctctct tgcaacaagg ccataagcct ctgcgccatt tgaagcgggg
    47701 tcgtaagtca tcactgtttg accataagag ggagcctctg agatgcgaac tgctcttgga
    47761 attggaatgt caattagctc agcgctgtaa tgttttctca cttctgctgc aacatcatta
    47821 gctagccttg ttcggccatc aaacatggta agtaaaattg ttgttagttt tatatttgga
    47881 ttcaagtgtt cttgaattaa tgaaacggtg tgaagcaata acgttaagcc ctctagtgag
    47941 taatactcgc attgaattgg aattaatagt tcttgcgcag caactagagc attaattgtt
    48001 aataagccta gacttggtgg acaatcaata attacataat caaatcgctc gcctttattt
    48061 gctgcatcga gtaaataatt atttaaagat ttttttaatc tcatctctct tgcaaccatt
    48121 ggaactaatt ccacttcggc atttgcaaga gattgtgttg caggaataac ttcaaggttt
    48181 ggatagcctg gaatttttag cgctgcagat tgcattggta gatctttaat aattacttca
    48241 tacattccat taacttcatc ccttgaaatc ccaaaagcgg tgctggcatt tccttgcgga
    48301 tctaggtcaa taattaaggt ttttaagccc cccatagcca gggctgccgc cacattgact
    48361 gcggaggtgg ttttgccgac cccgcctttt tggttggcaa cggtaaatat ccgagtagtt
    48421 atcggtttag ccataggctc tcggaagcgt ttggttccaa ttacctgcct aggggccgat
    48481 gtttcacgtg aatcactcac gccacctagt taaccagcct taaccgcctc aatgatgcgt
    48541 cctgtcggca agtcatcaag atttatctcg tgtagttgat gggatttgaa ctttttgagg
    48601 ttagttgtgg ccatctcttg agctgcagac tcgcctttga tagcaagaag agatccccct
    48661 ggcgcaatta aatgccaagt gctctcgatt agacgaggaa ggggggcaac cgctcttgct
    48721 aaaacgaagt tatagctgcc ttttactgat tcggccttag ctctaataac ctcgacgttt
    48781 tctagtttta gggcctctat tacttcatat agaaactcaa cccttctttg aaggggttca
    48841 attaggctta ctttgagctc ggggcgagct agagcgacca caattccagg taggccagcg
    48901 cccgagccaa tatctgccac ctttgccccg ctcatcggaa gcagggttac taccggaagg
    48961 cagttttcaa tatggcgttg ccagatccgc tccccttcgg ctgggccgat taggcctcgc
    49021 tcaatagcgg tagtcgctag gagcttttcg aaagctaggg cctcttcagg cttatctgga
    49081 aagtagcgtt tagccctagt ttcacgtgaa tcaatcaggc tggcatcacc acaacgcagc
    49141 gatctggatc ttcgccttca gattcgctag ttaggccaat tttttgaatg gtgtcatgga
    49201 tgactttccg ctcaaaggca ttcatcgggg cgagctttat cggcttgcct tttgatttga
    49261 cctcatctgc tgtctcttca gcaagctttg caagcgcgct ctttttatct gctcgaaacc
    49321 catcgatatc tagcattaac ctagagcgtt cacctgttgc ggtttgaaca gctaggcgag
    49381 tgagctcttg gagagcatcc agtacttccc catccccacc cactaggtgg gcaaggtcgc
    49441 ctccttcaat aactagcgct gctcgctcat tttcaacgct aatttcgatg tcgccatcta
    49501 gatctgcgat atcaagtaag ccttctaggt agtcagcagc gatatcgccc tcttcttcaa
    49561 ggcttgtcac tacagtcttt tcttcgctca tatcttctcc ttagtatttt gccccgtttt
    49621 gaggctttta atgctatttg cgccttttct tctttttctt ctttggttgt tccctctgcc
    49681 ctgttggttt tggctcctct atggcaactc ctccgccatt gttttcagcc tctggctcct
    49741 ggcctgactt ttttgctttc tttgcttgga gctcctccca ggcgggtgat cctggagttg
    49801 gatttctctt aatcacatag aactgctgac cccaggtcca gaaatttgtt gtggaccagt
    49861 aaatcaaaac tccgattgga aaattaactc ctgagacagc aaagataata gggaataaat
    49921 aaagcattat tttctgctgt tgcaacatca tattgtttga ggaatctagc tttggcattc
    49981 ctttaaccat taactgacgt tgagtagtaa atgttgttag cgacattaaa aagattagaa
    50041 atactgtcac tatctttact gttgtattag atgaaccaag aaatgtctgt gagataggag
    50101 ccccaaagaa ttccgccttt gccatctgcg ctgcaatttc gctagttaaa actccacgag
    50161 cttgtggcgg gttctttcca attccattaa gcactgtaaa gagcgcaaag aatattggag
    50221 cttgggcaag gattggaaag catgaagcca ttggatttgt cttatgggtt ttatatagct
    50281 tcattaactc ttctgattgc ttctggcgat catctttata tttctgttga atcttcttca
    50341 tctcaggggc tagggcagta agagctcgtt gagatttgat ttgttttaca aaaaggggaa
    50401 ttagaataat tctaattaaa actacaaggc ccataataga gagagtccat gtgactccac
    50461 ttgctccacc aaataccggc gacaatgcgc tatggattgc caccataacc ccagaaacaa
    50521 cccaataaag cggatctaaa attcccattt agctcaccat gctttctgtc tctttatttg
    50581 atagtaaaaa agggccaatt ttcaacgttt ttggtgggta atccactccg ccgtggctta
    50641 gtgggttaca tctaataact ctccatacgc ctagagctag accaaaaacg tggaagtttt
    50701 ttattgattc aaatgtgtat tgagaacagg aagggtaata cttacacctt ggcgcaagtc
    50761 caggggctat aaatttctgc caaattttaa ttattaaaat aaatggaagc ataagtaagt
    50821 ccagtaattt attaatcatc ttcccgcctt ttcatttaac gcggtaaata atcttggaat
    50881 ctcattatga acattctgct caggtttggt tgctctaatt accaccatgg attgtggggg
    50941 aagaatatga agactgctct ttgaagcatg tcttagctgc cttgtaactc tatggcgaat
    51001 tactgagcca ccgatcgctc ttgaaacaac aaaaccaatt tttggagata agagatttgg
    51061 ttctttactt aaataaccaa ccaggctctt tgttgttgaa cgaattggag aacgagtaac
    51121 tcgcgcgaag tcttcgcgag aagttaggcg attaatggct gctagcacgc cggttattac
    51181 agcttaggcg gatatacgag cgcgaccctt agcgcgacgg gcagagagaa ctgcgcgtcc
    51241 accacgagtt gccatacgag cgcgaaaacc atgcttcttc gcgcgccgac gattattcgg
    51301 ctggaaagta cgcttcatct tcttctctca ctcctttaaa aacagctttt aatggaagct
    51361 gtcgggattg atcaggtctt gaggtaatcg cgtagctgcg attgggggcc acaagagcag
    51421 ggggcaacgg tatctgttga cctaccgatt tggaaaactg gggatatctt ttagggcatt
    51481 tagggccttt gggaagattt tgtggataac cacttgcttt tattgggata aaggcatact
    51541 ttccgcctct atccacaggc gctaaggttt ttgagctctt ttccccagag attaagccta
    51601 aagtcgaagt atagaccaca gcctgtggat aactttgtgg ataactagtg agatgagggg
    51661 ttatgtcaat tgctgaacct ggagcccttg aagccaagga aatcgaggct gaccttttag
    51721 agctttggaa gggaattgtt gattctgttg ctaaagaatc tcctcaaaac cgagcatttc
    51781 ttaccctcac taaacctcta ggtttattaa agagcgatgg gggatcaaat ctccttcttg
    51841 ccgcccctaa tgttttcgca aaggatgttt tagagagccg cttaaaggtt ctattaaacg
    51901 atgcgctatc tgccaagatg ggcgagaaaa taaatatcgc agttactgtt gatgaatctt
    51961 tagatgccga cctatctgtg gttgaagagg tagatactga agttgaatcc ccaagggcag
    52021 gaactggccg ccaagaagtg cagagtaaaa gttctgatca aacccagcta aaccctagat
    52081 atatttttga aacttttgtt attggcgcat ctaatcgctt tgcccacgcc gcggctgttg
    52141 ctgtcgctga agctcctgcg aaggcttata acccgctctt tatttatggt gagtcaggtc
    52201 ttggaaaaac ccatttatta catgcaattg gggcttacgc caaagagtta tatggcggcg
    52261 ttcgggttcg ctatgtctca tctgaagaat ttacaaatga ctttattaac tcaattcgcg
    52321 acgataaatc ctctaatttt cagcgtcgtt accgcgatct tgatgttcta cttgttgacg
    52381 acattcaatt tttagagaat aaagagagaa cccaagaaga gttcttccat acttttaata
    52441 ctttatataa cgctaataaa cagattgtta tctcaagtga tcgcccgcca aaacaattaa
    52501 ccactttgga aga
//
LOCUS       DAOROD010000008        38116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_27_length_38116_cov_27.882609, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000008 DAOROD010000000
VERSION     DAOROD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 38116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38116
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_38116_cov_27.882609"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLW53_02990"
     CDS             complement(<1..656)
                     /locus_tag="PLW53_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRD36420.1"
                     /translation="MCRLMAYSSKTSVNLPEFMGQGFSDFLKLSEVHNDSWGLALANP
                     DSATVIKEVASAAASARFSEVISNQSAPGALLHFRWASPGLEVTHENAHPFCFEDIAF
                     IHNGALSPYEAVKSLIAPEFESLREGDTDSELFFLYLLTEIKANGFVEGVVKGIKNLK
                     ENFEYSSINSMIINSDYLIVVSEHDPLNKPSWTDDLYYELRYRIDENGVAVASSGWDQ
                     "
     gene            686..1903
                     /locus_tag="PLW53_02995"
     CDS             686..1903
                     /locus_tag="PLW53_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRD36421.1"
                     /translation="MLLTALVVIVLAAYIIESILDNLNLSTARNALDPKIAHLYDAKE
                     RERSISYSAEKTRFGFISSTISTLILIFALSYGWFASLDNWARGIVDNQILVSLLFIA
                     SLSVISYLLNLPFTLYGTFKIEEKYGFNKTTPKVFFTDTIKGAALATLIGGSLLTAVL
                     WFYQQLGSNFWILAWALLALFSLLMFMFGTTLILPLFNKLEPIKDGPLKQGIEKYCAS
                     QGYNLGRLFVMDGSKRSSKANAFFSGLGSSKTIVLFDTLIEKLSTTEIIAVLAHEIGH
                     YKKKHTLSMFIFSNIQTFATFALLGFLLGYPELSTALGASESSFHLSALAFFILFTPL
                     SIFLGLINNTWSRHNEYEADTFAKQTYKVEPMRSALSKISTDSLANLSPHPAYVAFNY
                     THPTLLQRLKNLE"
     gene            complement(1949..3379)
                     /gene="glnA"
                     /locus_tag="PLW53_03000"
     CDS             complement(1949..3379)
                     /gene="glnA"
                     /locus_tag="PLW53_03000"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRD36422.1"
                     /translation="MFKNSAEVLNYIKKENVALVDVRFTDLPGMQHHFNVPVSQFTED
                     VFKDGLMFDGSSIRGFQSIHESDMKLLPAPETAFIDPFRTEKTLVLHFSVVNPDDNSP
                     YSRDPRQIVNKAVEYMRSTGIADTAYFAPEAEFYVFDRVKFQTRMNTGFYEVDSYEGA
                     WNTGIDDDPDGTPNRGYRTRIKGGYFPVSPTDQFADLRDQMVMNLAKVGLDVERAHHE
                     VGTAGQMEINYRFNDILHAGDDLMKFKYIIKNTAWAAGKTVTFMPKPLFGDNGSGMHV
                     HQSLWKDGKPLMWGDGYANLSDTARWYIGGLLKHAPSLLAFTNPTVNSYKRLVPGYEA
                     PVNLVYSARNRSACIRIPITGSSPKAKRIEFRCPDPSSNPYLAFAAILMAGLDGITNK
                     IEPPKPVDKDLYELEGAEAKAIAQVPGSLDAVIDNLERDNDFLTRGGVFTKDLIDTWI
                     DWKRKSEIDYVRLRPHPAEFELYYDI"
     gene            3536..3907
                     /locus_tag="PLW53_03005"
     CDS             3536..3907
                     /locus_tag="PLW53_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36423.1"
                     /translation="MERAGLGRRVLALSIDWAIASFTAALVYPLQASALGPSLFRLGV
                     FVLEVSLLTSLGGASAGQRVMGLRVLSWPDNLFLRPGPLLLRTILIALVIPAVVTDKD
                     GRGLHDRAAKSVVMRVSKKRA"
     gene            complement(3904..4587)
                     /locus_tag="PLW53_03010"
     CDS             complement(3904..4587)
                     /locus_tag="PLW53_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HRD36424.1"
                     /translation="MFGRKKKDPNAPKKVRFKTIRDAYSLARKHYKFVFLRCLAVFAP
                     LWGAGIGIGAFFNRPGYAAFLTFPLAFLGAFFYFTRLAGNAAYASIEGQPGAGASVLM
                     SIRKGWTITPAVNVNKNQDMVHRAVGKAGVVLVGEGGHALRSLLTDERRKMERFAPGV
                     PIHELVIGNEKDQVSVRKLQKRMKKFPKKLSTVQVRELRARLKSVGGLNIPLPKGPMP
                     KNMRMPKPR"
     gene            complement(4591..5322)
                     /locus_tag="PLW53_03015"
     CDS             complement(4591..5322)
                     /locus_tag="PLW53_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD36425.1"
                     /translation="MADLVYPPVIKLVRAFWKYLDLRFTFYGQSHIPNKGPAILAMNH
                     VGYLDFALAGTAFLPTSRLVRFMAKKEIFDHPIAGPLMRGMKHISVDRNNGAPSFLAA
                     LKALDKGEIVGVFPEATISQSFELKDMKSGVIRLAMESGAPILPMVIWGSQRVWSKKM
                     PKNLSRSSIPIFIAIGPLRYIKKGSDIDRELAALKEAMAQLLNQVQSDYPDSHEGARW
                     APARLGGSAPSLAELEDMRRNKKES"
     gene            complement(5315..6250)
                     /gene="lipA"
                     /locus_tag="PLW53_03020"
     CDS             complement(5315..6250)
                     /gene="lipA"
                     /locus_tag="PLW53_03020"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRD36426.1"
                     /translation="MLSPDGRKLLRIEARNAETPIERKPEWIKTKAKMGPQYSALRSL
                     VAKEGLHTVCQEAACPNIYECWEDKEATFLIGGSACTRRCDFCNIDTGKPEALDRDEP
                     RRVAESVATMGLKYATVTGVARDDLEDEGAWLYSETIRQIHQVVPGCLVEMLAPDFSA
                     KPELLSEVFESRPEVFAHNLETVPRIFKRIRPAFNYERSLSVITQARDFGLITKSNLI
                     LGMGEQRSEVSQALLDLRSAGCELITITQYLRPSNLHHPVERWVKPQEFVELAKEAEE
                     LGFLGVMSGPLVRSSYRAGRLYKQAIAARQGISNG"
     gene            complement(6250..6918)
                     /gene="lipB"
                     /locus_tag="PLW53_03025"
     CDS             complement(6250..6918)
                     /gene="lipB"
                     /locus_tag="PLW53_03025"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRD36427.1"
                     /translation="MISQALASTREEASIINCQSLGLIDYEEALAIQRQLHQEVVDQI
                     SASSLLLLQHPPVFTAGRRTLDSERPIDGSKVIDVDRGGKITFHGPGQIVGYPIVKLK
                     KPNDVVGFVRQLEKALIQICQDFNIAGQTYCERSGVWVRDERGDRKIAAIGIRVAKGV
                     TMHGFALNVNPDLSFFDRIIPCGIADAQVTSMEKELNRSISLEQVQEVVEARMKQSLS
                     QVSQ"
     gene            7056..9245
                     /locus_tag="PLW53_03030"
     CDS             7056..9245
                     /locus_tag="PLW53_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRD36428.1"
                     /translation="MSQEVLSEKKKSGVKGRKALWIAIVSILVWLSIGGFAGGAFSKI
                     STVQENDNSAFLPDSAESTIAGEVLVKFSSQDDQLFPALLLLLGDLNPATNAQAFERV
                     NQYSATLLSKTLPATGKPLSTYFARGVPLTPIPSADGKAILINAQLDFAVADANIDGE
                     PIFPKIIEFIREDMEKEFSSAGITTHVTGPAGLFADLFEAFGSIDTRLLQTTLIVVAI
                     ILIVVYRSPVLWILPLFTAASALGIATMIVYYLAREDIIDLNGQTQGILDVLVLGAAT
                     DYALLLIARYREELHQHQSRFEAMKIALRGVVEPIIASGSTVIAGLMVLLLSDLSSNR
                     GLGPVGSIGIASSMLAVLTLLPALLIVFGRWIFWPKIPRFGDVDEKLSGIWSKVGSLV
                     DRRPKAVWISTALALLIFAGFSTTLKSDGLSQSEAFTTRTDSVIGLEKLGEHFPSGEG
                     TPVEIVVDQADIASAAAAIGRVSNVASVVPLTNVDPVTQRPTSQLKVVDGKVVLYATL
                     KVAPDSAEGKESIPLIRQAAKQVNPNILVGGQSAIGYDVDQSSRRDNRVIIPIVLLLI
                     AVILGFLLRSILAAALLLGTVVLSFAATLGVCALVFDHVFGFAGTDAAFPLFAFIFLV
                     ALGIDYNIFLMTRVREESLKIGTRAGIIKGLTVTGGVITSAGIVLAATFGVLGILPLV
                     FLAELGFAVAFGVLLDTIIVRSLLVPALVRVIGPKIWWPSKLQHQEN"
     gene            9257..10270
                     /locus_tag="PLW53_03035"
     CDS             9257..10270
                     /locus_tag="PLW53_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD36429.1"
                     /translation="MKAGIIGYGLAGRYFHGPTLLSAGFHVTAICARSLEKKAAAHQD
                     FPNAVIVNSIDELVAEDLDLIVVASPNEFHSEHALASIRAGINTVVDKPMGRDYYETL
                     ELYDAAEQSGVLLTVFFNRLWDSDSLTIKKIIEQGEIGNIFRLESRFERFRPEINPLA
                     WRESTSFEAGGGLLLDLQSHLVSTALDWFGPAELVFSSVRSVRGASDDDVLLVLKHDN
                     GVDSYLSASAISGNPGPKIRLNGDKASLVITELDKQEEILRRGFRAQAGQWIDGDEIT
                     SSAKIYKGSDSFSYRGEAGFYPAFYNEVRLAIEYGGELPVSRELSLSVARIIDQARQI
                     SIR"
     gene            complement(10274..11164)
                     /locus_tag="PLW53_03040"
     CDS             complement(10274..11164)
                     /locus_tag="PLW53_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRD36430.1"
                     /translation="MSLPQRIAITGASGLIGTALVGHLKSEGHTVQKLVRRKVVSPDE
                     VFWDPRSGDIDLAALDGVDAIIHLAGANVGDKRWTKKYRAEILNSRLLGTTTIANAVS
                     NLKPDVFISASAIGWYGETGNRAVSESDRPGDDFLAAVCKQWEAAADLATNTRVVKIR
                     TGLVLDSTGGALGKMLPLFKFGVGGKLGSGKQWWSWITLHDLVEAICFLLEKPVSGAV
                     NLTAPNPATNQEFTSALARALKRPALIPVPGFALKIALGGFSSEVLGSKKVLPTALLD
                     AGFNFDYPHLAPALSALLAE"
     gene            complement(11165..>12253)
                     /gene="sucB"
                     /locus_tag="PLW53_03045"
     CDS             complement(11165..>12253)
                     /gene="sucB"
                     /locus_tag="PLW53_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HRD36431.1"
                     /translation="PTPAPAPVVQAPIVQAPVVQAPVAPVVAVEKPVPAPPKTPPPLL
                     PKPTAGSVLDDSYVTPIVRKLASENGVDLSRVSGTGVGGRIRKEDVLAAVSRPTQVAA
                     SAAAQTFSAPSEVRTQSVAVSPLRGTTVSMSRLRKVIAARMLESLSVSAQLTTVIEVD
                     ITKIARLREKAKATFEGREGVKLTYLPFFAVAVCEALKQHPVVNASVEGDQITYHGAE
                     HLGIAVDTERGLLVPVIRDAGDLNMGGIARRIVDVASRTRDNKISPDDLGGGTFTITN
                     TGSRGALFDTPIINQPQVAILGVGAVVKRPMVVKGEDGGETIAIRSMVYLALSYDHRI
                     VDGADAARFLTTLKDRLEEGRFESDLGL"
     assembly_gap    12254..12353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12354..12621)
                     /locus_tag="PLW53_03050"
     CDS             complement(<12354..12621)
                     /locus_tag="PLW53_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36432.1"
                     /translation="MSFSVKMPALGESVTEGTVTRWLKAEGDQVALDEPLLEVSTDKV
                     DTEIPSPAAGKLEKILIPVDSVVAVGTELALIGDGASSPSAPVAA"
     gene            complement(12706..14073)
                     /gene="lpdA"
                     /locus_tag="PLW53_03055"
     CDS             complement(12706..14073)
                     /gene="lpdA"
                     /locus_tag="PLW53_03055"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRD36433.1"
                     /translation="MADFDLVVLGGGSGGYACALRAAQLGLKVALIEKEKLGGTCLHR
                     GCIPTKALLHAGEIADGARHSAEFGVKAEFHSMDMVAVNAYKDGVISKLHKGLQGLVK
                     SRNITYVEGEGKLVSATTVKVGSESYIGKNVVLATGSYAKSLPGLDIDGKQVITSDQA
                     IHMGYVPKSVIVLGGGVIGCEFASVWKSFGAEVRIIEALPHLVALEDESSSKALERAF
                     RKRGINFELGVKFKSVSKSASGVEVLLEDGKSFAADLLLVAVGRGPTSSGLGYEDVGV
                     KMDRGYVLVDNKCRTNIKGVWAVGDLIPTLQLAHVGFAEGIMVAEEIAGLNPRPINYD
                     GVPRVTYSEPEVASVGLTTAEAKKRGIEIKELNYDLAGNGKAQILKTAGAIKLVADSS
                     GTVIGIHMVGSRVGELLAEAQLIFNWQADADDVAQHLHAHPTLSEAMGEAHMALAGKP
                     LHAHG"
     gene            complement(14121..15626)
                     /locus_tag="PLW53_03060"
     CDS             complement(14121..15626)
                     /locus_tag="PLW53_03060"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRD36434.1"
                     /translation="MPTTLPTISASDNSVIDEVLVLGFALDKKSKKFSILSGELRVDQ
                     GPLLQALTDLGASGASDEVIKLPGSASKLILLTALTLKADGSIAHEVLRRAAGAAARS
                     LAGTTSATFALPQASKQDFQAIAEGALLGAYTFDEFRGSSKADRKPALKKITIYSKKA
                     KDKSYLQAIKRAEIIAKYTHLTRDLINTPPSHLTPDSFSLRFKKLAASLGVKVEILNE
                     TQLIKGGYGGIIGVGQGSANPPRLLRLSYSGKGAKKRYAFIGKGITFDTGGLALKPAQ
                     GMEAMKSDMSGAAAVCAAVLAIAELKLPINVDAWAALAENMVSDKATRPSDIITIYGG
                     KTVEVLNPDAEGRLVLADAMVRAAEVGKKAGGLDGMVDVATLTGAQVVALGTRTSAVM
                     TNNESFREQFLTLAEITGEAFWPMPLPEELRASLDSPVADMANIGDRMGGMLVAGLFL
                     KDFVDSELPWLHLDIAGPAYNEAKAHGYTPVGGTGVALRTLVALAESSAAK"
     gene            15644..16744
                     /gene="gcvT"
                     /locus_tag="PLW53_03065"
     CDS             15644..16744
                     /gene="gcvT"
                     /locus_tag="PLW53_03065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRD36435.1"
                     /translation="MSKYSPLHQFHLDSGAKVADFGGWQMPIEYPAANGGGVLNEHSA
                     VRNTVGLFDVSHLGKASVIGEGALDYLNSIFTNDLRKISDGQAQYTLLCQKDNGGVID
                     DLIIYRYSSTHFLLIPNAANCQQVLDQISKEAPSTLEIKNLHERYALFALQGMKSLDV
                     LKDLGIDVNLEYMSFVESQLDSETLIICRTGYTGEHGYEILTSWASARKIWDLLLEKV
                     KKYSGLPAGLGARDTLRTEMGYALHGHELSTDITPVEASASWAVAFDKEFWGKSVLEK
                     QRAEKKHRRLAAILVNDRGIPRAGMEIKDKEGKKIGYVTSGTFSPSLKSGIALGLFDT
                     QIPLESQVFIDIRGRLSQGVIKRLPFQPSKVK"
     gene            complement(16707..17513)
                     /locus_tag="PLW53_03070"
     CDS             complement(16707..17513)
                     /locus_tag="PLW53_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRD36436.1"
                     /translation="MRKTRAIASILSWFPIFIGSTLGLLAFTWPLFIPESNFFLLKPD
                     SARFLALFIAFLAVLVISVEISRGALDSKIVALLGVLAALIAALRLVGAGAVGVEPMW
                     FLLIISSYIFGSKFGFSLGVISMAVSAVISGGIGPWLSFQMLAAGWIGLFAGLFSRKI
                     NKRFEIITLVIIGVICSLLFGALMDLQLWPWIASTNTQLGFIAGAPLMENLSRYLTFH
                     LATAMAWDIPRAITTALLIALSARALLASLSRAAMRMGLTSPSMVEKVNA"
     gene            complement(17500..19107)
                     /locus_tag="PLW53_03075"
     CDS             complement(17500..19107)
                     /locus_tag="PLW53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36437.1"
                     /translation="MITFSSVSLIYPNAQRTIVEDISFTVNEGELLLLIGHTGAGKSS
                     LLKLINGLIPHHTGGILTGEISVAGRSTRALKPGELADVVGIVGQNPINGFVSDIVED
                     EIAFGLESLGIAPEIMRKRVEEVLDLLALAPLRRRNISELSGGEQQRVAIAAALVTNP
                     KVLLLDEPTSALDPIAAEEVLSILHRLVHDLGLTVILAEHRLERVIGFVDRIILIHGD
                     GSVDLGPARQILSSSPIAPPLVELAKKLELGEIPLSIREFRKIAPEISEAPTKDIEKL
                     KGDAILTINSVSASYDQDQASKALDDISIEINGGEIVALMGRNGAGKTTLLKCAVAQA
                     PLTKGEVRVNQRDPKSFTDKELIRFVGYIPQDAGDLLYGQSIKQECDLADFDNGVEPG
                     TTLAIYKDLMKIPSLNSHPRDISEGQRLGLALSIILSAQPALVILDEPTRGLDYEAKK
                     VLIKILKQIASSLDRAVLISTHDVELVAEVADRVIFLADGEVIADGSSREVLKASPAF
                     APQVSKALPNNSWLSLSDVERYLSEKN"
     gene            complement(19104..19946)
                     /locus_tag="PLW53_03080"
     CDS             complement(19104..19946)
                     /locus_tag="PLW53_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRD36438.1"
                     /translation="MIKTLRASKESFHPLFWWLWALTLLVILLLSDSALASIAISVGA
                     VALVLMKRSNTYWYQSFRWSLRLAALAFALRMAIGVIIGVPMPGQVLFTIPSIKLPDL
                     FVGIRLGGDVTSQRLSTAFDEATLLVALILIFAAASALSNPHELLRVLPRKYYGIGLA
                     TVIASSVAPQSARSIERVRAARRLRGESSTGIVSLRKVGIPVLEESLERSIDLAASLE
                     SRGYGYFPNPSRYRPQSWRLRETFALAAPIYALIFLFLVPAVSSALLAGLVVIAVITP
                     GFIS"
     gene            complement(19963..20637)
                     /locus_tag="PLW53_03085"
     CDS             complement(19963..20637)
                     /locus_tag="PLW53_03085"
                     /inference="COORDINATES: protein motif:HMM:NF040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO2322 family protein"
                     /protein_id="HRD36439.1"
                     /translation="MLSKFINLNILKTYPKLIALSILILSLLAPIPSHGAKGYRYWGY
                     FQAAAGASTWSAAMTGPSVEVKDGDVEGWAFVFSSNDIPAVEPMMDPDFATLCGETPE
                     TSGKIRVGLVVDFGAGNIAPDGEVPNEFFSDCVVVDKGSVGLDVLEAALDVRAGDSGL
                     ICGIAGYPASECGAEIDEPVAEEVVAIDATSEPESSSSQSAPILLAVLGLFLLSAFFL
                     NRRRTR"
     regulatory      complement(20710..20855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20887..21888
                     /locus_tag="PLW53_03090"
     CDS             20887..21888
                     /locus_tag="PLW53_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HRD36440.1"
                     /translation="MEYRKLGSSGMYISEITYGNWITHGSQVEQDAAIKCVRTAYDLG
                     ITTFDTADVYAGTRAESVMGKALKGIRRESYELFTKVYWPTGSGKNDRGLSRKHIMES
                     VHASLKRLNTDYLDLYQMHRFDYETPLEESLSAFDDLIRQGKVHYIGFSEWDATQISS
                     ALKIQDAKGYTRFVSSQPQYSMLWRVIESKVDPLCRNEGIGHIVWSPIAQGALTGKYL
                     PGKKPPKGSRATDKKGGANMISRWMKDDVLTAVEKLKPIAEKEGLTQAQLAVAWVLQN
                     PNISSAIIGATKPSQIKENVKASGVKLSSETMKAIDDALGNLPERDAAKTVSPNPRA"
     gene            complement(21889..22407)
                     /locus_tag="PLW53_03095"
     CDS             complement(21889..22407)
                     /locus_tag="PLW53_03095"
                     /inference="COORDINATES: protein motif:HMM:NF022686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HRD36441.1"
                     /translation="MAEKKGRPTPKRKEVEQSRVINRLAPAANKEAKKLQKAQARILR
                     AQQRDAYMRGDENALPPRDKGPVRRFIRNYVDSRRSVGEFFLPIIVLVLFMTLIPVMY
                     IQFAAIALMYSALIFSVVDGYFLSRRIKREVSTRFPGETLKGLGMYGWLRSTQIRRLR
                     APLPQVKRGEKI"
     gene            complement(22414..23559)
                     /gene="nadA"
                     /locus_tag="PLW53_03100"
     CDS             complement(22414..23559)
                     /gene="nadA"
                     /locus_tag="PLW53_03100"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRD36442.1"
                     /translation="MASITDLLLLGKGSDLSSERGVSCTGELPASSDPHLVERAKKAR
                     AALGNSAMILGHHYQRDEVIDFADITGDSFKLAQAAADNQSAENIIFCGVHFMAESAD
                     ILTSANQRVILPDLAAGCSMADMAAANQVNQAWKVFEKLGIASKTIPVTYMNSSAAIK
                     SFTGENGGTICTSSNAERAMKWAFERGEKVFFLPDQHLGRNTAVLKLGLKLSDCVIWN
                     PWKVNGGLSDEELTSAKVILWRGHCSVHGRFSVENIEQVRQKIADVKVIVHPECQYEV
                     VSKADVIGSTEMIIKTVTQSPAGSSWAVGTELNLVKRLAANNPDKTVVFLDKDVCYCS
                     TMNRIDLPHLLWAMESLVKGRVVNQIKVEPKTANFARIALDRMLGLP"
     gene            23639..23983
                     /locus_tag="PLW53_03105"
     CDS             23639..23983
                     /locus_tag="PLW53_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRD36443.1"
                     /translation="MSTETKTEGIKLSSEAAVKVASLLAQEGRDDLSLRVAVQPGGCS
                     GLRYQLYFDDRSLDGDTVMTYGSVKVVTDKMSTPYLMGATIDFVDTIEKQGFTIDNPN
                     AGGSCACGDSFN"
     gene            complement(23954..25045)
                     /locus_tag="PLW53_03110"
     CDS             complement(23954..25045)
                     /locus_tag="PLW53_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36444.1"
                     /translation="MLPITDIAPEDDFQSELPLEPMARQHLLELFDSAWFDPAKIHRN
                     SAQLRNLINEARESIASHLGIASSELEIVGELGFGFQCALAGLLTESKSKFIYSRVDR
                     QVIHAFARQHQARGGEVLEQSVDSKGIVDFQVDSSREATLSFQAVNRETGVIQNAPKT
                     SDKTAVFADMSSAYPLSNLPDNWDSAIWDPKYFGGPSGIALVGIANSGRWQNPGPIID
                     KRRVYGSFSKPLLLATAVALENWTKRAREDFVKLTELNSFIRQELASQIKDIKIAGDG
                     NNCDPRFLAFVIPGTHSEEVLRKVEAQGVLIDAGSACGSGPLSPSHVLTAMGYSENGN
                     YRITLKPAHNRHSLKKLVSVLVKGIAAGA"
     gene            25102..25851
                     /gene="coxB"
                     /locus_tag="PLW53_03115"
     CDS             25102..25851
                     /gene="coxB"
                     /locus_tag="PLW53_03115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRD36445.1"
                     /translation="MPALKKLALAASLPLLAGCSNEELTGIGYRGGVTSVNEASFALW
                     QWAWLAAAVVGAFTLILILWPVFFHRKKEGEAFPRQFQYNIPAEVAYTVIPFIIVAVL
                     FGYTAKAENEIVALSPQSSTELHDVTVNGFQWSWQFTYKEAGEGTTVTGTPGQPPVLY
                     MPLGEKVRFTFTASDVIHSFWVPDFMIQMQNIPGVTNQLEFTANELGEYRGFCNILCG
                     RQHSQMRFTVKVVTPSEYDSYIQSLKAEVNS"
     gene            25848..27512
                     /gene="ctaD"
                     /locus_tag="PLW53_03120"
     CDS             25848..27512
                     /gene="ctaD"
                     /locus_tag="PLW53_03120"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRD36446.1"
                     /translation="MTTYATRTLASDEMPEAPKPMSKGKLAVKILTSTDHKVIGYLYL
                     GTSFAWFLIGGILALLLRAELTRPGMQFLSSEQYNQVFTMHGTIMLLMFATPLFVGFA
                     NVIMPLQIGAADVAFPRMNMLSYWLYLFGGIVAFAGFLTPGGAASFGWTAYAPLSNDI
                     YSPGIGADLWIMGLAVSGLGTILGGVNFVTTIFTMRAPGMTMFRMSIFSWNVLLTSIL
                     VLIAFPPLAAALLALESDRLFGSLIFDPANGGAMLWQHLFWFFGHPEVYILALPFFGI
                     ASEIFPVFSRKPIFGYKGLVAATIAIAALSVAVWAHHMYTSGQVLLPFFSFMTFLIAV
                     PTGVKFFNWIGTMWRGSLTFETPMIWVIGFLVTFLLGGLTGVILASPPLDFAVSASYF
                     VVAHFHYVLFGTVVFAMFAGFYFWWPKMTGRMLNETLGKIHFWTLFLGFHTTFLIQHW
                     LGIKGFPRRYADYLATDGFTWMNMVSTLGSTLLAISMIPFAINVWITRKSPKVESNDP
                     WGYGSSLEWATTCPPPRHNFLRMPEIRSERPAFDLNHPNHSKIEGH"
     gene            27513..27914
                     /locus_tag="PLW53_03125"
     CDS             27513..27914
                     /locus_tag="PLW53_03125"
                     /inference="COORDINATES: protein motif:HMM:NF023691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HRD36447.1"
                     /translation="MKVGYRLFLFLFVFYAIVGGVYWYLGGEPLGITAVLLCAGLALI
                     IGYYFWFIDRRSGGVLPEDNNDGLISDGAGELGFYSPHSWWPLPVGLAATAAGVGLIV
                     GWWLTLIAIGALLVSVMGFVLEYEKPSASSH"
     gene            27914..28384
                     /locus_tag="PLW53_03130"
     CDS             27914..28384
                     /locus_tag="PLW53_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36448.1"
                     /translation="MEKIRAEIVDTGIELFYKTQLEIKAPAKVIFDLIADPKNHQKID
                     GSGMLKGELKAPDRLYLGASFGMKMKYVIPYIIKNHVVEFEENKSLAWRHLMHNVWRY
                     ELVELDPRTTRVIQTWDGRKARSKWWVSDSYQWVPRAMAKTLVRLKEMAEKHAS"
     gene            complement(28398..29990)
                     /locus_tag="PLW53_03135"
     CDS             complement(28398..29990)
                     /locus_tag="PLW53_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HRD36449.1"
                     /translation="MSARERVAGQVANYVDERTGSAKWLKKTLTKVFPDHWSFMLGEI
                     VLYSFIILLLSGTFLTFWYDPSMREVEYEGSYQPLSGLKMSAAYASSLHISFDVRGGL
                     LMRQIHHWAALIFMAAMVAHLMRVFFTGAFRKPREFNWIIGVGLLTLGIVEGFLGYSL
                     PDDLLSGTGIRIAEAIIQAIPVVGSYLSFFIFGGAFPGEMFIQRIYVVHILLLPGIFL
                     ALITVHLMLVWYQKHTQYPGPGRTEKNVVGYPLMPTYMAKAGGFFFIVFGVTAFLGAV
                     ASINPIWIYGPYNPGQIGAGSQPDWYMGWLDGLVRMAPPLEAYIFGYTLSLNILIPGL
                     IIPGIIFTGMALYPFIESWITGDKREHHLLDRPRNAPNRTALGAMSITFMLVTLINGG
                     NDLLATHFDLSINQIMWFSRIGVIVLPPLAFVITKRICLSLQRADRELVLHGKETGRL
                     VMLPHGEFIEVHEPLTPEKAYLLTQHEQLPALEANLSNEYGVRNPKAIRSKIRSRLSR
                     SQAEQIAKPTANDVKELEGGHH"
     gene            complement(29990..30982)
                     /locus_tag="PLW53_03140"
     CDS             complement(29990..30982)
                     /locus_tag="PLW53_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRD36450.1"
                     /translation="MSKDLAPLSDPGLPEHIHRKADVDVRAAKKAERQVGILFLFSVL
                     GTLLFVYAYLGIDEDAFIFVPVLGSTNAHQLFLGLGLAMALFFIGMAAVHWAKTLMPD
                     HEVIDYRKEQRSKDEDREDFVSTVKDRANEAGLGRRPFIKRTMLLSLGLVGISPVLLL
                     GDLGPLPGNDQKETNWKSGTRLVTDPGDRPIRPSDLEVGGVAQVQPELPAGKVRHLDD
                     IAKDSVLLIRIRPEEFNLDAERLSWTYEGIIAFSKICSHMGCAVALYEQTTKHLLCPC
                     HQSTFDVTRAAKVIFGPSARPLPQLAITVDSQGYLVAKQPFSEPVGPSFWGRNK"
     gene            complement(30987..31781)
                     /locus_tag="PLW53_03145"
     CDS             complement(30987..31781)
                     /locus_tag="PLW53_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD36451.1"
                     /translation="MRFISKYRRHRFAGPLLLLGALLFAGLTYSAAQAVTEQATLSQS
                     DKATLIAEGKEIFARGCSSCHGLNAEGAGVGPSLIGVGAASVDFQVATGRMPVADMSQ
                     QIARKEPVYNDQEVAALAAYVASLAPGPAAVTNEELAWERDGNMAEGGELFRNNCAMC
                     HNLAGQGGALTQGKYAPTVMGVEVKHIYEAMITGPQAMPVFSETILTPEEKLSIIKWI
                     KHAETEPQLGGAALGRVGPVTEGLVGWVLGLGLLIGVAVWLTAKAR"
     gene            complement(31782..32408)
                     /locus_tag="PLW53_03150"
     CDS             complement(31782..32408)
                     /locus_tag="PLW53_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HRD36452.1"
                     /translation="MSSAVSTPPRLNRPNIVAVGTIVWLSSELMFFAALFAMYFTTRS
                     VQGPVIWGQGVEMLNIPFSALNTTVLVLSSVTCQFGVFAAERFQNARTGSLLQLSKWG
                     MREWFALTFVMGAFFIGGQVYEYAELVHEGLTISSAAYGSVFYLATGFHGLHVTGGLI
                     AFLIVMIRVSKARRFGHSQATTAIVVSYYWHFVDIVWIALFSAIYLVA"
     gene            32552..32962
                     /locus_tag="PLW53_03155"
     CDS             32552..32962
                     /locus_tag="PLW53_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002547844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD36453.1"
                     /translation="METSQKTLKIALYSDDSSVRSSVITALGKQISPDLGAHKILEFA
                     TAKALRMAVDSGANFDLFILDGEAVPEGGLGVAKQLKDEVFNCGVVIVLVARSSDAWL
                     AGWSRAEGVITHPIDPFTLAKNVAEILAKNPVAS"
     gene            32981..34036
                     /gene="trpD"
                     /locus_tag="PLW53_03160"
     CDS             32981..34036
                     /gene="trpD"
                     /locus_tag="PLW53_03160"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRD36454.1"
                     /translation="MELTVSWDEIVEKLTAKQDLSDLQITWAMAQILDGLATNDQIKS
                     FLLLLKAKGEKPSEVRALVDVMYQRGIAIDIPDRAVDIVGTGGDGASTINISTTAAII
                     TTAAGARTIKHGNRAASSKSGAADLLEALGIDISLGAKQIQECVRKIGIGFAFAPVFH
                     PAMKNAASARKELATPTVFNILGPLANPAKPKAVAIGVARAELLELVATVLAERDCEG
                     FIFRGDDGLDEISLSTTSTVYQINKGEISKEIFDPSELGLERIGIDELRGGTPSQNAD
                     ITRAIFAGKGGPYRQAVLLNAAVAIAAFKADFHLGITQQFANGYTLAKQAVDSGAAAQ
                     LVERWANLSTELAKSSL"
     gene            complement(33979..35835)
                     /locus_tag="PLW53_03165"
     CDS             complement(33979..35835)
                     /locus_tag="PLW53_03165"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HRD36455.1"
                     /translation="MRTLKLIRLNVINPIYLCSMKSEQESQFNSAFQPSFDEISPSLY
                     ETTFVVVDLETTGGSHNSAAITEIGAVKVRAGEIVGEFQTLVNPESPIPPFITVLTGI
                     TDAMVIAAPKLGEAIFSFLEFVGSSKDSVLVAHNAPFDIGFLKAACAKLEISWPSYQV
                     LDTAKIARYVVTRDEAPNCKLSTLSTFFGATTNPDHRALSDARATVDVFHGILDRLGS
                     YGVKTLDELKSFSARITSAQRSKKYLAEGLPNTPGVYIFRDEAGIPLYIGTTRNLRNR
                     VRSYFTAAETRKRIHDMISLAHSLDVIQTATIIEAEVRELRLISEKQPRFNRRSKFQA
                     KSLWIKLTKEAFPRLVAIRGAKGLDDESGWAGPFNGRDESARAIEALYEVTQIRQCKP
                     RITLTSMKNASPCALFDMKRCGAPCIGQESIDSYSSHVTAIHQSLHIDSRPILNSLNE
                     RMKELANQERFEEAAEVRNRLGALIRGSARAQSIRSLTRVKELIAAIATSIDTWEFVV
                     IRHGKLAASATAKSDNYKEVVQSLKLTAEVVIDNGEILPASHHEEVEVLLRYLNQEGI
                     RIVEIDGIWALPTFGSAAARSEIEKVRAQVGANSYKEDFANSVDRFAQRSTN"
     gene            35836..36606
                     /locus_tag="PLW53_03170"
     CDS             35836..36606
                     /locus_tag="PLW53_03170"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD36456.1"
                     /translation="MAIEGAPPGWLSDYRAKGSGSNADVGVLLVHGFTGSPASMRPFA
                     HFLNERGYQVSVPVIPGHASRWQDLNKVHWSSWPQKVEQELIELKKTCRKVFIVGLSM
                     GGGNSLYVAADHQDKVDGIVLVNPMIHIPGLQIKFVSIISKVQKSRASVGDDIKKPGV
                     TEWGYDALPIKGVAQLYKYLKLARKRLPGVKTPVLLFHSVDDHTLDVSNTEIILNEIG
                     SSDKQRIELLNSYHVATLDYDADIIFENTRLFIQERSK"
     gene            36603..>38116
                     /locus_tag="PLW53_03175"
     CDS             36603..>38116
                     /locus_tag="PLW53_03175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRD36457.1"
                     /translation="MSQNPFLTKSNLEYELPPFAEISEEDYLPAFYAGMDEQLAEVEK
                     ITDQAEVTFENTLVALEASGGILSRVAAVFYNKSSSDTNDAIDKIEAEIAPKLAAHSD
                     KIKLNQKLFARITQLVNSGLDLNSEDAWLLKRYHRDFLHAGAGLSDSQREEIKRINEE
                     LSTLQTEFSKRVLSETNDLAVFVDDIKELDGLSENQIKACKAAAEARGLQDKYMIGAV
                     NFTGHPLLRSLKNRELRKKIMQASLSKGLRGNDFDTRQVLLKMVKLRAKRAEIFGKKS
                     HAQYSLSEQTAGSPERVHDMLKKIAPAALANAKAEGRDLERLAKLDGIEKLESWDWDY
                     YTEKVRAEKYDLDTALMKPYFELESVLFKGVFYAAGKLYGMSFKERPDLVTYHPEARA
                     FEVFNEDGSKIGLYIADFYTRDSKRGGAWMNSLVKQSHLLNQLPVVVNNLNIPKPPKG
                     EATLLTLDETTTLFHEFGHAIHGLLSNVKYPRFSGTAVQRDFVEFPSQVNEMWIL"
BASE COUNT        10472 a         8587 c         8456 g        10501 t
ORIGIN      
        1 ctttgatccc agcctgatga ggccaccgca actccgtttt catcgatgcg atagcgcagt
       61 tcgtagtaga ggtcatcggt ccaacttggc ttattcaatg gatcatgttc gctcaccaca
      121 attagatagt cagagttgat aatcattgaa ttaattgagc tatattcaaa attctcctta
      181 agattcttta tccctttaac tacgccctct acaaatccat tggctttgat ctcagttaag
      241 agatagagaa agaagagctc gctatcggta tcaccctctc taagtgattc aaattctgga
      301 gcaatcagtg atttaacagc ttcataaggg ctaagggcgc cattatggat aaaggcaata
      361 tcttcaaaac aaaatggatg agcattttcg tgagttactt caagtccagg ggaggcccat
      421 ctaaagtgca gcagagctcc aggcgcgctt tgattactta ttacttcgct aaatctggcg
      481 ctagctgcag cgcttgctac ttccttaatt accgttgcgc tatctggatt agcaagggca
      541 aggccccagg aatcattatg aacctctgag agtttaagaa agtcgctaaa gccctgaccc
      601 ataaattcag gtaggttgac tgaagttttt gaactatagg ccattagtcg acacataggg
      661 tcagcctatc ggctacccta agaaaatgct tttaactgcc ttagtagtaa tagtccttgc
      721 cgcttacata atcgaatcaa tcctggataa cttaaaccta tccactgcaa gaaatgcctt
      781 agatcccaag attgcccatc tctatgatgc caaggagcgg gagaggtcaa ttagttacag
      841 cgcagagaag acccgttttg gctttatatc ttcaactatc tcaactctaa ttctcatctt
      901 tgctctgagc tatggctggt ttgcatcgct tgataattgg gcaagaggca tcgtcgataa
      961 ccagattttg gtatcccttc tctttatcgc ctcactcagt gttatctcct atctattaaa
     1021 cctgccattt actctttatg gaacttttaa aattgaggag aaatatgggt ttaataaaac
     1081 tactcctaag gtctttttta cagacactat caaaggcgca gctcttgcaa ccttaatagg
     1141 gggcagcttg ctaacagcag ttctctggtt ctaccaacag cttggctcaa atttctggat
     1201 tcttgcttgg gcgcttctag cacttttctc gctactaatg tttatgtttg gaacaacatt
     1261 aattctgccg ctttttaata aattagagcc aattaaggat ggaccactaa agcaaggaat
     1321 tgaaaagtat tgcgcttcgc aaggctataa cttgggtcgc ctatttgtaa tggatggctc
     1381 gaagagatca tcgaaggcca atgcgttttt tagtggcctt ggctcaagca agaccattgt
     1441 cttatttgat accttgattg agaaactgag cactacagag atcattgcag ttttggctca
     1501 tgaaattggt cactacaaga agaaacatac tctctcaatg tttatcttta gcaacatcca
     1561 aacctttgca acctttgctc tccttgggtt tctacttggt tatcctgagc tctcaaccgc
     1621 actaggggca agtgagagta gctttcatct ctcagcgcta gcattcttca ttctctttac
     1681 tccacttagt attttcctag gtttaataaa caacacctgg tctcgtcata acgagtatga
     1741 ggccgatacc tttgctaagc agacttataa agttgaacct atgcgaagtg ctttgagcaa
     1801 aatctccacc gatagcctcg caaacctttc ccctcaccct gcttatgtgg cattcaatta
     1861 cactcatcca acacttctgc agagattgaa gaacttagaa taaaaaagag ggcctgccta
     1921 agcagaccct ctcttctaac tcttgagttt agatatcgta atagagttca aactctgctg
     1981 gatgtggacg caagcgaaca taatcaattt ctgatttacg cttccaatca atccaggtat
     2041 cgatcaagtc tttagtaaag acaccgccgc gagttaagaa gtcgttatct ctttcaaggt
     2101 tatcgataac agcatctagt gagccaggta cttgggcaat tgccttagcc tcggctccct
     2161 ctaattcgta gagatcttta tcaactggct ttggtggctc aatcttgttt gtgattccat
     2221 caagaccggc catcaagatc gcagcaaatg caagatatgg atttgatgat ggatctgggc
     2281 aacggaactc aatgcgcttt gccttagggc tagatccggt aattgggata cggatacagg
     2341 ctgagcggtt acgagctgag taaacaagat ttactggagc ttcatatcct ggaacgagtc
     2401 gcttataaga gttcactgtt gggttggtaa atgcaagcag tgaaggtgca tgctttagaa
     2461 gacctccgat ataccagcgc gcagtatcag aaagatttgc ataaccatcg ccccacataa
     2521 gtggtttgcc atctttccaa agagattggt gaacatgcat tccagagcca ttatcaccaa
     2581 agagtggctt tggcataaag gtcacagtct ttccagcggc ccaagcagtg ttcttaatga
     2641 tgtatttaaa cttcattaga tcatctccgg catgaaggat gtcgttgaag cgatagttga
     2701 tctccatctg acctgcagtt ccaacttcat ggtgagcgcg ctctacatca agaccaactt
     2761 tggcaagatt cataaccatc tgatcgcgca gatcagcaaa ttgatcagtt ggagagactg
     2821 ggaaataccc gcctttaatt ctggtgcgat agccacgatt tggtgtgcca tctggatcat
     2881 catcaattcc ggtattccac gctccttcat atgaatcaac ttcatagaag ccggtattca
     2941 tacgagtttg gaatttaacg cgatcaaata catagaactc agcctcagga gcaaagtatg
     3001 cggtatctgc aattccagta gatctcatat attccacagc cttattaacg atctggcgtg
     3061 ggtctctgct atacggtgaa ttgtcatcag gatttacgac tgagaaatgt agaactagag
     3121 ttttttcagt tctaaatgga tcaataaatg ctgtctctgg tgctggaaga agtttcatat
     3181 ctgattcatg aattgattgg aaacctctaa ttgaggaacc atcaaacatc agaccatctt
     3241 taaagacatc ttcagtaaat tgtgagacgg gaacgttgaa gtgatgctgc ataccaggca
     3301 gatcggtgaa gcggacatca acgagtgcga cattttcttt ctttatgtaa tttaaaactt
     3361 ctgcagaatt tttaaacatt tctctcctga ggtttttctt catctttagc tccacgttga
     3421 gctctctagc aatggcccaa acctaggcta gggcggtgaa atatccataa ccggtgggtt
     3481 acggctatgt ttcataaaac gggcctaagg gagctttttt agttaggttg agcctatgga
     3541 acgggcagga cttggccggc gagtgctggc gctaagcatt gattgggcca ttgcatcatt
     3601 tactgcggcc ctggtttatc ccttgcaagc aagcgcgctt ggcccatcgc tgtttcgcct
     3661 tggagtattt gtcttagagg tttccctact tacatcactt ggcggggcat ctgcagggca
     3721 gagagttatg ggcttaagag ttttaagttg gccagataat ctatttcttc gccccgggcc
     3781 acttctgctt cgcacaatcc tcatagcact tgttattcca gccgttgtta ctgataaaga
     3841 tggcagggga ctacatgatc gagctgctaa atctgttgtg atgagagtta gtaagaaacg
     3901 agcttaacga ggcttaggca tcctcatgtt cttaggcatc ggaccttttg gaagcggaat
     3961 attaagtccg ccaactgatt taagtcttgc tcgcagctct cgcacctgaa ctgttgagag
     4021 tttcttagga aactttttca ttctcttctg tagttttcta accgagactt gatctttttc
     4081 atttccaatt acaagttcat ggattggaac accaggggca aagcgctcca tcttgcgcct
     4141 ctcatctgta agtagggacc taagagcatg gccaccttcg ccaactagaa ctactccagc
     4201 tttaccaact gcgcgatgaa ccatatcttg gttcttatta acatttactg ctggagtaat
     4261 ggtccagcct ttacgaattg acattaaaac actggctcca gcgccaggct gtccctcaat
     4321 tgatgcatag gcagcatttc ctgcaagtcg cgtgaagtaa aagaaagcgc caagaaaagc
     4381 tagtggaaag gttagaaaag ctgcataacc tggtcggtta aagaatgctc caattccaat
     4441 tccagcgccc caaagtggcg caaagacagc taggcagcgg agaaagacaa acttgtaatg
     4501 cttgcgagcc agggagtaag catcgcgaat cgttttaaat ctaactttct taggagcgtt
     4561 gggatccttc ttctttctac caaacatctc tcaactctcc tttttatttc ttcgcatatc
     4621 ttcaagttct gccaggcttg gagcgcttcc gccgagcctt gctggcgccc atctagcgcc
     4681 ctcatgggag tcaggataat cactctgaac ttgatttagt aattgagcca tcgcttcttt
     4741 aagagcggcg agctctctat cgatatcaga gcctttcttg atatatcgaa gtggcccaat
     4801 ggcaataaag atagggatac tgcttcgaga taagttcttc ggcatctttt tgctccacac
     4861 tcgctgcgag ccccagatca ccatcggaag gattggagcg ccagattcca tagctaggcg
     4921 aataacgccg ctcttcatat ctttaagttc aaagctttga gaaattgttg cttccggaaa
     4981 aacgccaacg atctcacctt tatcaagggc cttaagagct gcaagaaaag agggggcgcc
     5041 attattacga tcaacgctga tatgtttcat accgcgcatt agcggaccgg ctattggatg
     5101 atcaaagatt tctttctttg ccataaatct aactaagcga gaggtgggaa gaaaggctgt
     5161 tccagctagg gcaaaatcaa gatagccaac atgattcata gcaagaattg ctggaccctt
     5221 atttggaata tgactctgtc cgtaaaaagt gaatcgaaga tcaagatact tccaaaacgc
     5281 acgaactagt tttataactg ggggatagac cagatcagcc atttgaaatc ccttgacggg
     5341 cagcgatggc ttgcttatat aaccttcctg ctcgataact tgatctaaca agtggcccgc
     5401 tcatcactcc aagaaaacct aactcctctg cctcttttgc taattcaaca aattcctgcg
     5461 gcttaaccca tctttcaaca gggtgatgca aattagaagg tcttagatat tgagtgatag
     5521 tgattaactc gcaacctgcg cttcttaaat caaggagtgc ttgcgatact tcacttcgct
     5581 gctctcccat accaagaatc agatttgact tggtaatcag gccaaaatct cttgcctggg
     5641 taataacgct caaagatctc tcataattaa atgcaggtct aattctttta aatattctcg
     5701 gaactgtctc aaggttatgg gcaaatacct caggcctact ttcaaaaact tcacttaaaa
     5761 gctcaggctt tgctgaaaag tcaggggcca gcatttcaac caggcaccca ggaactacct
     5821 ggtgaatctg gcgaatagtt tcgctatata gccaggctcc ttcatcctct agatcatctc
     5881 ttgcaacacc ggtaacagtg gcatatttaa gtcccatcgt tgctaccgac tcggcaactc
     5941 ttctcggttc atctcgatcg agggcttcag gttttccggt atcgatattg cagaaatcgc
     6001 accttcttgt gcaggcgctg ccaccgatta aaaatgtggc ttctttatct tcccagcatt
     6061 catagatgtt aggacaagcc gcctcttggc agacagtatg caaaccttct ttagcaacaa
     6121 gtgagcgaag tgcgctgtac tgcggcccca tttttgcctt tgttttaatc cactctggct
     6181 ttctttcaat gggggtttca gcattgcgcg cctcaattct tagtaactta cgtccatcag
     6241 gggaaagcat tattgactca cctgactaag tgattgtttc attcgcgcct ctacgacttc
     6301 ttgaacttgc tcaagtgaaa tacttcgatt caattctttc tccattgaag tgacctgcgc
     6361 atcagctatt ccgcagggga taatccggtc aaagaagcta aggtcggggt taacatttag
     6421 cgcaaagcca tgcatcgtta cgccctttgc aactctgatt ccaatggctg caatctttcg
     6481 atcgcctctc tcatcgcgaa cccaaacgcc agatcgctcg caatatgttt gccctgcaat
     6541 gttgaaatcc tgacatattt gaatcaaagc tttttcaagc tgtctaacaa atccgacaac
     6601 atcatttggc ttttttaatt taacaatcgg atatcccaca atttgtcctg ggccatggaa
     6661 tgtgattttt ccgcctctat caacatcgat aacttttgaa ccatcaattg ggcgctcact
     6721 atctagcgtt ctccgcccgg cagtaaatac cggtggatgt tgtagcaaaa gaagtgagct
     6781 agcacttatc tgatctacaa cctcttggtg aagttgacgc tgaattgcta gcgcctcttc
     6841 ataatcaatt aatccaaggc tctggcaatt gataatcgag gcttcttctc tcgtgcttgc
     6901 cagggcttgt gaaatcacag gtagagccta actttataga gcagttcttg gcgcaatgag
     6961 cggtagaatt acgctcctta ggctaaagga ttatgggtgg gcagtagcca aaatcacccc
     7021 aaaactatcg aggcgcgaga ttacaaggag atgctgtgag tcaggaagta ttgagcgaga
     7081 agaagaagtc cggcgtaaag ggtcgcaagg cgttatggat agcgattgtc tcaattttag
     7141 tctggctatc aattggcggc tttgctgggg gagctttctc caagatttct acagtccaag
     7201 aaaatgacaa ctctgctttc ttgcccgata gcgctgaatc aactatcgct ggtgaagtat
     7261 tggttaagtt ctcatcccag gatgatcaac tatttccagc actgctctta ttacttggag
     7321 atttaaaccc tgctactaat gctcaagcct ttgagagagt gaatcagtat tcagctacgc
     7381 tgctaagtaa aacccttcct gccactggaa agccattaag tacctacttt gccaggggag
     7441 ttccgctaac tccaattcca tctgcagatg gcaaggcaat cttgataaat gcgcaactgg
     7501 attttgcagt tgctgatgcc aatattgatg gagagccaat ctttccaaag attatcgaat
     7561 tcattagaga agatatggag aaagagtttt cttctgcagg cattactaca catgtaacgg
     7621 gtcctgccgg gttatttgca gatctatttg aagcctttgg ttcaatcgat accaggctat
     7681 tgcagacaac tctgatagtt gtggcgatta ttcttattgt ggtttatcgc tctccagtgc
     7741 tctggatatt gccgttgttt accgcagcaa gcgcgctggg gattgccacc atgattgtgt
     7801 attacttggc aagagaagac attattgatc taaatggcca gacccaagga attcttgatg
     7861 ttttggttct gggagctgcc actgactatg cgctgttatt aattgcccgt tatcgcgaag
     7921 agctccacca acatcaatct agatttgaag cgatgaaaat tgcgctgcgc ggagtagtag
     7981 agccaattat cgcttcagga tcaactgtta ttgccgggct tatggttctg ctacttagtg
     8041 atctttcaag taatcgcggc cttggccctg ttggatcaat aggaatcgcc tcttcaatgc
     8101 ttgcggtttt aactctgctt ccagctttat taattgtctt tggtcgctgg attttctggc
     8161 caaagattcc acgctttggt gatgttgatg agaaactctc gggaatatgg tccaaagttg
     8221 gaagtttagt tgatcgtcgc ccaaaggctg tttggattag cacagcactt gcgctgctta
     8281 tctttgctgg attttctacc acattgaaat ctgatggact ctcacagagt gaagccttta
     8341 ctactcgaac tgattcagtt attggactag aaaagctagg agagcatttc ccaagtggtg
     8401 agggaactcc agttgaaatt gttgttgatc aagcagatat tgcctctgct gcagcagcaa
     8461 ttggaagggt ttcaaatgtt gcatcagtag tgccgctaac aaatgtagat ccagttacac
     8521 agcggccaac ttcgcaactt aaagtagttg atggaaaagt agtgctctat gccaccttaa
     8581 aagttgctcc agattctgct gaaggcaaag agagcattcc attaattcgc caagctgcta
     8641 agcaagttaa tcccaatatc ttggtaggtg gacagagcgc aattggctat gacgtcgatc
     8701 aatcatcacg tagagataat cgagtcatta ttcctattgt gcttcttttg attgctgtga
     8761 tccttggctt cttgcttaga agtattttgg cagcggcgct cttattagga actgtagttc
     8821 tctcctttgc tgcaactctt ggagtctgcg cgctggtctt tgatcatgtc tttggatttg
     8881 ccggaactga tgcagccttc ccgctctttg ccttcatatt cctagttgca cttggcattg
     8941 attacaacat cttcttaatg actagagttc gagaagagtc gctaaaaatc ggaacccgag
     9001 ctggaatcat taaaggctta actgttactg gcggggttat tacctcagct ggaatagttc
     9061 ttgcagcaac ctttggagtc cttggaatct tgccgctggt cttcttagca gagctgggat
     9121 ttgctgtcgc atttggggtg cttctagaca ccatcattgt tcgctcactt ttggttccag
     9181 ctttggtgcg agtaattgga ccaaagatct ggtggccatc aaagcttcaa catcaagaga
     9241 actaatcttt cgctaaatga aggcaggaat aattggctat ggattagctg gtcgctattt
     9301 ccatggccca acacttcttt ctgctggatt tcatgtaaca gccatatgtg cgagatccct
     9361 tgagaaaaaa gctgctgccc atcaagactt tccaaatgct gtgatagtta attcaattga
     9421 tgagctggtt gcagaagatc tagatttgat tgttgtggca tcaccaaatg aatttcacag
     9481 cgagcacgca ctcgcgtcaa ttagagctgg aatcaataca gttgtagata aaccaatggg
     9541 acgggattac tacgagactc tggaacttta tgatgcagct gagcaaagcg gtgtgctctt
     9601 aacggtattt ttcaatcgac tctgggactc tgattcctta accataaaga aaataatcga
     9661 gcagggagaa attggaaata tttttcgtct cgagtcaaga tttgaaagat ttagaccaga
     9721 gattaatcct ctagcttggc gcgagagcac ctcttttgag gctggtggcg ggctactact
     9781 tgatctgcaa agccatttag tatcaactgc tcttgattgg tttggcccag ctgagttggt
     9841 attttcaagt gtaagaagtg ttcgcggcgc aagtgatgat gatgtccttc tggttttaaa
     9901 gcacgataac ggagttgatt cttatctctc agccagcgca attagcggaa accctgggcc
     9961 aaagattcgg ctcaatggcg ataaagctag tttggtaatt actgaacttg ataagcagga
    10021 ggagatttta agaaggggat ttagagccca agctggccag tggatagatg gcgatgagat
    10081 tacaagtagc gcaaaaattt ataagggaag tgactcattt tcttatcgag gagaagctgg
    10141 tttctatcct gctttttata acgaggtaag gcttgctatt gaatatggcg gcgagctacc
    10201 agtaagtcga gagttatcac ttagcgttgc cagaattatt gatcaggcgc gccaaataag
    10261 tattaggtaa ggcctactct gcaagtagcg cacttagcgc tggagctaag tgaggatagt
    10321 caaaattaaa accagcatct aaaagcgcag ttggcaaaac cttctttgag cctagaactt
    10381 cacttgaaaa tcctccaagg gcgattttga gagcaaaccc tggaactgga attagcgcag
    10441 ggcgctttag cgctcttgca agtgctgaag taaattcttg atttgttgct ggatttggag
    10501 cggtgagatt tactgcgcca ctaactggct tctccaatag aaagcagatc gcctcaacaa
    10561 gatcatgaag tgtgatccag gaccaccatt gctttccact acctaatttt ccgccaacgc
    10621 caaacttaaa gagcgggagc atctttccta acgctccacc agttgaatct aaaactagtc
    10681 cggttctaat ctttacaact ctagtatttg tggcaaggtc ggctgctgcc tcccactgct
    10741 tgcaaacggc tgctaaaaaa tcatctccag gtcgatcaga ttcactcact gctctattgc
    10801 cagtttcgcc ataccaacca atcgcgctcg ctgagataaa tacatcaggc ttaagatttg
    10861 aaacagcatt agcaattgtt gttgtgccaa ggagtcgaga atttaaaatc tctgctcgat
    10921 atttcttagt ccatctctta tcgccaacat ttgcgccagc aaggtgaata attgcatcaa
    10981 caccatcaag ggctgctaaa tcaatatctc cacttctagg atcccaaaag acttcatcgg
    11041 gggagactac ttttcgtctc acaagttttt gaacggtgtg gccttcagac tttagatggc
    11101 cgacaagcgc cgtgccaatt aaacctgaag caccagtgat ggcgatgcgc tgtggcaggg
    11161 acatttacag ccctagatca gattcgaaac gaccctcttc taaccgatct ttaagagtag
    11221 ttaagaatct tgcagcatct gcgccatcaa caattcgatg atcataagaa agtgcaagat
    11281 agaccatgga gcgaatagcg attgtctctc cgccatcttc tcccttaaca accattgggc
    11341 gcttaacaac agcgccaact cccaagattg ctacctgagg ctggttaatt attggagtat
    11401 caaagagtgc tcctcgggat ccagtatttg tgatggtaaa tgtgccgcca cctaaatcat
    11461 ctggcgagat cttgttatct ctagttcttg aagccacatc aacaattctt cttgcaatgc
    11521 cgcccatatt tagatctcca gcatcacgaa tcactggaac taatagaccg cgctcggtat
    11581 caactgcaat acctaggtgt tcggctccat gataagtaat ctgatcgcct tctaccgaag
    11641 catttacaac tggatgttgt ttaagtgctt cacatactgc cactgcaaaa aatggcagat
    11701 aagttaattt cacaccttcg cgaccttcaa aggttgcttt agctttttca cgcagacgtg
    11761 caatcttggt gatatcaact tcaataactg ttgttagctg ggcgctaaca gagagggatt
    11821 caagcattct tgcagcgata acttttctaa gtcttgacat gcttactgtt gtgccacgaa
    11881 gcggggatac cgcaacagat tgggtgcgaa cttcactcgg agcagaaaaa gtttgcgctg
    11941 cagctgaagc agccacttga gttgggcgag atacagctgc caaaacatct tccttgcgga
    12001 ttcttccgcc aactccagtg ccactgactc gagagagatc aacgccattt tcactggcaa
    12061 gttttctaac aataggagtt acataggaat catcaagcac agatccagct gtaggttttg
    12121 gaagaagtgg cggcggagtc tttggcggag caggaactgg cttttcaact gcaaccactg
    12181 gagcaacagg tgcttgcaca acgggggcct gaacaatcgg tgcttgcaca actggtgcag
    12241 gcgctggtgt tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagctgc
    12361 aactggagca ctaggtgatg atgcgccatc accgattagg gcaagttcag ttccgacagc
    12421 aacgacgcta tctactggaa ttaaaatctt ctctaattta ccggcagcag gtgatggaat
    12481 ttcagtatcg actttatcgg ttgagacctc tagtaacggt tcatcgagtg caacttgatc
    12541 accttcggct tttagccaac gggttacagt tccctcagtt acgctctcac cgagagctgg
    12601 catctttact gaaaatgaca ttttctctaa acgccccttt gttggtatct aatacttatt
    12661 ctaacgagca atccatctga tgataaaaag cctaaccgaa actctctagc catgagcgtg
    12721 aagcggcttt ccagcaaggg ccatatgagc ctcacccatc gcctcagaga gggttggatg
    12781 agcatggaga tgttgagcaa catcatcagc atcagcctgc caattaaaaa tcaattgtgc
    12841 ctctgcaagg agttcgccaa ctcttgaacc aaccatatgt ataccgatga ctgttccgga
    12901 gctatcagca actaatttta tcgctccagc agtttttaga atctgagcct taccattgcc
    12961 agcaagatca taattaagct ctttaatttc aatgccgcgc ttttttgcct cagcagttgt
    13021 tagtccaacg gatgcaactt caggctccga ataagtaact cttggaacgc cgtcgtaatt
    13081 gatcggccgt ggattaaggc ccgcaatttc ttcagcgacc attattcctt cagcaaaacc
    13141 aacatgagct agttgcaatg tagggattaa atccccaaca gcccaaacac cctttatgtt
    13201 ggtgcggcat ttgttatcta ctaatacata gccgcgatcc atcttgactc caacatcttc
    13261 atagccaagg ccagatgatg tagggcctcg tccaactgca acaagcaaaa gatcagcagc
    13321 aaagctcttt ccatcttcga gtaatacctc aactccggat gcgctcttac tcacgctctt
    13381 gaatttaact cccagttcaa aattgattcc acgttttcta aaggcgcgct ccaaagcctt
    13441 gctagatgat tcgtcctcta gggcaactag atgtggcagg gcctcaatga ttcgcacctc
    13501 tgcgccaaat gatttccaga ctgaggcaaa ttcgcagcca ataactccgc cgcctagaac
    13561 gattacagat ttaggaacat atcccatgtg aatcgcttga tcgctggtga taacttgctt
    13621 tccatcaata tcaagtccag gaagactctt tgcataagaa cctgttgcta gaacgacatt
    13681 ttttccaatg tagctctcag agccaacctt gaccgttgtt gctgaaacca attttccttc
    13741 accttcaacg taggtgatat ttctagattt aactaaacct tgcagacctt tatgaagctt
    13801 tgagataaca ccatctttat aggcatttac tgcgaccata tccatggagt gaaattcagc
    13861 ttttactcca aattcagcag agtgccttgc gccatctgca atctcgccag catgaagcaa
    13921 agctttagtt ggaatacatc ctctatgtag gcaggttccg ccaagtttct ctttctcaat
    13981 taaggcaacc tttaacccta attgggctgc tcgtagtgcg caggcataac cgccgctacc
    14041 accaccgaga actactagat cgaaatctgc cacatcaaac ctttctataa ttctttctca
    14101 atgagcctat cttgccagca ttacttggca gctgatgact cagcaagggc tactaaagtg
    14161 cgaagtgcga cacctgttcc gccaactggg gtatagccat gtgcttttgc ctcgttgtag
    14221 gcagggcctg caatatcaag atggagccaa ggcagctctg aatcaacaaa gtcttttaga
    14281 aatagcccag caactaacat cccgcccatt cgatcaccga tatttgccat atcagcaact
    14341 ggtgaatcaa gagatgctcg tagctcctct ggaagcggca ttggccagaa tgcctctcca
    14401 gtaatttctg caagagttaa gaactgctct ctaaaactct cattgtttgt cattaccgca
    14461 cttgttctcg ttccaagtgc cacgacttga gctccagtaa gagttgcaac atctaccatc
    14521 ccatcaagtc cgcctgcttt ctttccaacc tctgctgcgc gaaccatggc atctgcaaga
    14581 actaggcgac cttctgcatc tggatttaat acttcaacag ttttaccgcc ataaattgtg
    14641 atgatgtcac ttggcctggt tgctttatcg ctaaccatat tttctgccag ggccgcccaa
    14701 gcatcaacat tgattggaag ttttagttca gcaattgcta atactgcagc gcagactgca
    14761 gctgctccac tcatatcaga tttcattgcc tccatacctt gagcaggctt tagggcaagg
    14821 cctccagtat caaatgtgat tccttttcca ataaatgcat aacgtttctt agcgcccttg
    14881 ccactatagg agaggcgaag tagacgagga ggattagcag agccttggcc tacgccaatt
    14941 attccgccat aaccgccttt aattaattga gtctcattga gtatttctac tttaacgcca
    15001 agtgatgcag caagcttttt aaatcgaagt gaaaaagaat ctggagttag atgacttggc
    15061 ggagtattta ttaaatctct tgttagatgg gtgtatttag caattatctc ggcgcgcttg
    15121 attgcctgaa ggtagctctt atcttttgcc ttcttggagt aaatcgtgat tttctttagc
    15181 gcaggttttc tatcagcctt agaacttcct ctaaattcat caaaggtgta tgcgccaagt
    15241 agggctcctt cagctattgc ttgaaaatct tgttttgaag cttgcggcag agcaaaagtt
    15301 gctgaggtag ttccggcaag agatcttgca gcagctccgg cagctcttcg caatacttca
    15361 tgggcaattg agccatcggc cttaagagtc agcgctgtta aaagaattaa cttacttgca
    15421 cttcctggaa gttttattac ctcatcactt gccccacttg ccccaagatc cgttagggct
    15481 tgaagcagtg gtccctgatc tactcgaagc tcgccgctta atatagaaaa cttcttactc
    15541 tttttatcta gagcaaagcc tagaactagg acttcatcta ttactgagtt atcggaggct
    15601 gaaatagtgg gcaatgttgt aggcattggg ctaggttatt ggagtgagca aatattcgcc
    15661 attacaccag ttccatcttg attctggggc caaagtcgct gattttgggg gatggcagat
    15721 gccgatcgaa taccccgcag caaatggtgg tggggtttta aatgagcata gcgcggttag
    15781 aaataccgtg ggcctctttg atgtctccca tcttggtaaa gcttcagtaa taggtgaggg
    15841 ggccctggat tacttaaatt cgatttttac taatgacttg agaaaaatct cagatggtca
    15901 agctcaatac acacttcttt gtcaaaaaga taacggtgga gtaattgatg atctaatcat
    15961 ctatcgctac tcaagtaccc actttctact cattccaaat gccgctaact gccagcaggt
    16021 tttagatcag ataagcaaag aggcgccaag cacacttgag attaagaatt tacatgaacg
    16081 ttatgccctc tttgctctgc aagggatgaa atcacttgat gtcttaaaag atcttggtat
    16141 agatgtaaat cttgaataca tgtcatttgt ggaaagtcag ttggactcgg agacgctaat
    16201 tatctgtcga accggatata ccggagagca tggctatgaa attcttacct cttgggcaag
    16261 tgcgagaaaa atctgggatt tacttctaga gaaagtaaag aagtattccg gacttcctgc
    16321 aggacttggt gctcgcgata cgttaagaac tgagatggga tatgcccttc atggccatga
    16381 actctcaact gatataactc cagtggaggc aagtgcttca tgggctgtgg catttgataa
    16441 agagttttgg ggaaagagcg ttcttgaaaa gcaacgcgca gaaaagaaac atagaaggct
    16501 tgcagcaatt ttggttaacg atcgcggtat tcctagagct gggatggaga tcaaagacaa
    16561 agagggcaag aaaattggtt atgtaacaag tggcaccttc tcaccctcgt taaagagtgg
    16621 catcgctcta ggactatttg atactcagat tccacttgaa agccaggtat ttatcgatat
    16681 acgaggccgt ttaagtcaag gagtaattaa gcgtttacct tttcaaccat cgaaggtgaa
    16741 gtaaggccca ttctcattgc ggctctagaa agtgatgcca gtagagccct ggcgctaaga
    16801 gcgatcaaaa gggcggtagt gatagctctt gggatatccc atgccatagc tgttgctaag
    16861 tgaaaagtta gatagcgcga gagattctcc ataagcggtg cgccggcgat aaaacctaac
    16921 tgcgtattgg tagatgcaat ccaaggccag agctgaagat ccatgagcgc accaaagaga
    16981 agggagcaga taactccaat tattacaagt gtgataattt caaagcgttt gttgattttc
    17041 ctggaaaata aaccagcaaa caacccaatc catccagcgg ccagcatttg aaatgagagc
    17101 caaggtccaa ttcctccgct aattactgca gagacggcca tcgagataac tccaagtgag
    17161 aacccaaact ttgaaccaaa tatgtagctt gaaataatta ggagaaacca catcggttca
    17221 acgcccacag ctccagctcc cactaggcgc agggctgcta ttaaggctgc gagaactcca
    17281 aggagtgcaa cgatcttgct atcgagcgcg cctcttgata tctccactga aatcactaga
    17341 actgcaagaa atgcaataaa taaagctagg aatcgagcag agtctggctt taatagaaag
    17401 aaattactct caggaataaa gagcggccaa gtgaatgcaa gaaggccgag agtagatcca
    17461 ataaatattg gaaaccagga gagtattgaa gcgatagctc tagtttttct cacttaaata
    17521 tctttctaca tcacttagtg agagccagct gttattggga agagctttag aaacttgcgg
    17581 ggcaaaggca ggggaggctt taagcacttc tctactgctg ccatcggcaa ttacttcgcc
    17641 atctgcaaga aaaataaccc ggtctgccac ctcagcaact agttcgacat catgggttga
    17701 tatcaacact gcgcgatcaa ggcttgatgc aatctgcttt agtatcttta taagcacttt
    17761 cttggcttca taatcaagac ctctagttgg ttcatcgagt atgactagag ctggctgcgc
    17821 agagaggatg atgcttagtg caaggccaag tcgctgcccc tctgaaatat ctcttgggtg
    17881 tgaattaagg ctgggaatct tcatcaaatc tttatatatt gcaagggtag ttccaggctc
    17941 aactccatta tcaaaatcag ctaaatcaca ttcttgcttt attgattggc cgtagagaag
    18001 atcgccggca tcttgcggga tatatccaac aaatctgatt aactctttat ctgtaaaact
    18061 cttgggatct ctctgattta ctctaacctc acctttagta aggggagctt gagcaaccgc
    18121 gcatttgagt aaggtggtct ttcctgcgcc atttcttccc attagcgcaa caatctcgcc
    18181 gccatttatt tcaatactta tatcgtcaag agccttactt gcttgatctt gatcatatga
    18241 agcgctgaca gagttaatcg ttagtattgc atcgccctta agtttctcaa tgtccttagt
    18301 tggagcttca gatatttctg gagcaatttt gcgaaattca cgaattgaga gcggtatttc
    18361 accaagctcc aatttctttg ctaactcaac taagggaggg gcaattggag agctacttaa
    18421 tatctgcctt gcaggtccaa gatctactga gccatctccg tggattagaa taattcgatc
    18481 aacaaatcca ataactctct ctagtctatg ttctgccaaa atcaccgtga gcccaagatc
    18541 gtgaaccagg cgatggagaa tggagaggac ctcctctgct gcaattggat caagggcgct
    18601 tgttggttca tcaagaagaa gaacctttgg atttgtaaca agggctgcag caattgctac
    18661 tctctgctgc tcgccgccac ttagctctga gatatttctt cttcgcaaag gagcaagcgc
    18721 aagtagatct aatacttctt caacgcgctt tcgcatgatc tccggggcaa ttccaaggct
    18781 ctcaaggcca aaggcgatct catcttcaac aatgtctgaa acaaaaccat ttatgggatt
    18841 ttggccaaca attcccacta catctgctaa ctctccaggc tttaacgctc tagtacttct
    18901 accagccaca ctaatttcgc cggtgagaat tcctccagtg tgatgcggga taaggccatt
    18961 tattagtttg agaagcgagc tcttacctgc tcctgtgtgt ccgattagaa gaagtaactc
    19021 tccttcatta actgtaaaag atatgtcttc cacaatcgtt cgctgcgcgt tgggatagat
    19081 caaggagacg ctagagaagg taatcacgag ataaatccag gagttatcac tgctattacg
    19141 acgagcccag ctaatagtgc gcttgaaacc gctggaacta gaaaaagaaa aatcagagca
    19201 taaatcggtg ctgcaagggc aaacgtctct cgaaggcgcc agctctgcgg acggtatcga
    19261 cttggatttg gaaaatatcc ataacctcta gactctagag aggcggcaag atctattgac
    19321 cgctctaaag attcttccaa aacaggtata ccgacttttc ttaatgagac gattccagtt
    19381 gagctctcac cccttagtcg tcttgccgct ctaacccttt caatacttct agcgctctgc
    19441 ggcgcaactg agctagcaat aactgttgca agtcctattc cataatattt tcgcggcaga
    19501 actcgcagca gttcatgagg attacttagc gcgctcgctg cagcaaagat aagaattagc
    19561 gcaacaagca aagtcgcttc atcaaaggcg gtagagagtc gctgcgaggt gacatcaccg
    19621 ccgagtctta ttccaacaaa gagatctgga agtttgatgc ttgggatagt aaagagcact
    19681 tgcccgggca ttggaactcc aataattact ccgatagcca ttcgaagcgc gaaggctaga
    19741 gctgcaagcc ggagcgacca tctaaaggat tgataccaat aggtattact tcgtttcatg
    19801 agcactagag caactgctcc aacagatatt gcaatagatg caagcgctga atcagagagg
    19861 agaagaatca ctagaagagt aagcgcccag agccaccaga agagtgggtg aaaactctct
    19921 ttgctggccc tgagcgtctt aatcatttaa ttaaatttct tactatctag tgcgccggcg
    19981 attgagaaag aatgcactta gtaaaaagag ccctagaact gcaaggagaa taggagcgct
    20041 ctggctagag cttgattctg gctcggaagt tgcatcaatc gctactactt cctcagctac
    20101 tggctcatca atttcagcgc cgcattcact tgcaggatat ccagcaatgc cgcagataag
    20161 tccagaatct ccagctctta catcaagtgc tgcctctaat acatcaaggc cgactgagcc
    20221 tttatcaact acgacgcaat ctgagaagaa ctcattaggc acttcgccat ctggcgcaat
    20281 attgccagct ccaaaatcaa caactaaacc aactctaatc tttcctgaag tctctggagt
    20341 ttccccgcag agtgttgcga aatcaggatc catcattggt tcaactgctg gaatgtcatt
    20401 tgagctaaag acaaatgccc acccttcgac atcaccatct ttaacttcaa cagatgggcc
    20461 agtcattgca gcgctccaag tgcttgctcc agctgctgct tggaaatatc cccaatagcg
    20521 atatcccttt gccccatgag aaggaattgg agcaagaagg ctaagaatta atattgagag
    20581 tgcgattaac tttggataag tttttagaat atttagattt ataaatttgc taagcactgt
    20641 gaacctttca atagtagaaa agggctcacg atggcgaaaa aaaaacttcc gccccgtaaa
    20701 ccacgacggg tggtaaagat gcttcttggg tggtaatccg acttacctgc tcaatttcta
    20761 gcgggctcac ggttgcgggt cagcgtcgga ttttcaccga cttccccatt ccaaaaagct
    20821 attaagttgt gtgggctaat taatcaggaa agtggctaaa gcgcaaggag gcgctaggct
    20881 ctagttatgg aatatagaaa acttggcagc tctggcatgt atatctcaga gattacctat
    20941 ggcaactgga ttactcatgg ctcgcaagtt gagcaagatg ccgcaattaa atgcgttaga
    21001 acagcttatg acttaggtat tactactttt gacaccgctg atgtctatgc cggcactcgc
    21061 gccgagagcg tgatgggcaa agccctaaaa ggaattagaa gagagtctta tgagctcttc
    21121 accaaggttt attggccaac tggaagtgga aaaaacgata gaggcctatc acgcaaacac
    21181 attatggaat cggttcacgc atcgctaaag cgtctaaata ctgattatct agatctctat
    21241 cagatgcacc gattcgatta tgagacccca ctagaggaga gcttgtcagc atttgatgac
    21301 ttaattcgcc aaggaaaagt ccactacatt ggattttcag agtgggatgc aacgcaaatt
    21361 agtagcgccc taaaaattca agatgccaag ggctataccc gctttgtctc cagccagccg
    21421 caatactcaa tgctctggag agttatcgag agcaaggttg atcctctatg tcgcaatgaa
    21481 ggtattggcc atattgtctg gtcgccaatt gctcaaggag cgctaactgg aaagtattta
    21541 ccgggcaaga aaccacctaa gggatcaaga gctactgata aaaaaggtgg cgcaaatatg
    21601 atctcgcgct ggatgaaaga tgatgtttta actgcagttg agaaattaaa gcccattgca
    21661 gaaaaagaag gccttactca agctcagcta gctgtggcct gggtgctgca aaatccaaat
    21721 atttcatctg caatcattgg tgcaactaag ccttctcaga tcaaagaaaa tgttaaggct
    21781 tcaggagtta agttaagtag tgaaacgatg aaggcaatcg atgatgcgct tggaaatctt
    21841 cccgaaagag atgctgcaaa aactgttagc ccaaatccaa gggcttaact aaattttctc
    21901 gccccgctta acttgaggaa gtggggctcg tagccttcta atctgtgtag agcggagcca
    21961 gccatacatt ccaaggccct tgagcgtctc gccaggaaag cgcgtactta cctcacgctt
    22021 gatacgtctt gagagaaaat atccatcaac tactgagaaa attagagccg aatacataag
    22081 agcaatagca gcaaattgga tatacataac tgggattaga gtcataaata gaactagaac
    22141 aataattgga agaaagaatt caccgactga gcgtcttgaa tcgacataat ttcttatgaa
    22201 gcgtcttacc ggccccttat ctcttggagg tagcgcgttc tcatcaccgc gcatataggc
    22261 atcacgctgt tgagcgcgca aaatgcgagc ttgggccttc tgtaatttct ttgcttcctt
    22321 attagcagca ggtgcaagac gattaatcac tcttgattgc tcgacctctt tgcgctttgg
    22381 tgttgggcga ccttttttct ctgccatagt cgcttaaggt aggccaagca ttcgatctag
    22441 cgcaattcga gcaaagttag cagtcttagg ctcgacctta atctggttaa ccacgcgccc
    22501 cttaactaga gattccatcg cccagagtag atggggcaga tcgatgcgat tcatcgtcga
    22561 gcagtaacag acatccttat caagaaagac cacagtctta tctggattat tagctgcaag
    22621 gcgcttaact aaattgagct cagtcccaac tgcccaactg cttccagccg gagattgagt
    22681 aacagtctta ataatcatct cagtgcttcc aatgacatca gcctttgaaa caacctcata
    22741 ctggcactca ggatggacta tgactttaac gtcagcaatt ttctgcctaa cctgctcaat
    22801 attttcaact gagaaacgac catgaaccga gcagtggcct cgccataaaa taactttggc
    22861 agaagttagc tcctcatcac ttagcccacc attaactttc caaggattcc aaataacaca
    22921 atcgctaagt tttagcccaa gttttagcac tgcagtattg cgaccaagat gttgatcggg
    22981 aagaaagaag actttctctc ctctttcaaa agcccatttc attgctcgct ctgcatttga
    23041 tgaggtgcag atagttccgc cattttcgcc agtaaatgat ttaatcgcgg cagatgaatt
    23101 catataggta actggaattg ttttacttgc aatccctagc ttctcaaaga ctttccaagc
    23161 ttgattgact tgattagctg ctgccatatc ggccatagaa caaccagcag cgagatcagg
    23221 aagaattacg cgttgatttg ctgaggtgag aatatctgcg ctctctgcca tgaagtgcac
    23281 gccgcaaaag attatgttct cagccgattg attatcagca gctgcttgag ctaatttaaa
    23341 agagtctcca gtgatatctg caaaatctat tacttcatct ctctgataat gatggcccaa
    23401 aatcattgca ctattgccta aggctgctcg agccttcttg gcccgctcaa ctagatgggg
    23461 atcacttgat gctggaagct cgccagtgca ggagacgcca cgctcgctag agagatctga
    23521 gccttttcct agaagaagga gatcagtgat tgaggccatg gcttcattat ctcgcgaaag
    23581 ttcgctgcgt gtgcgctaaa gtatggctct aagaagttta cgagcgagga agagatatat
    23641 gagcaccgag accaagaccg aaggcatcaa actatccagc gaggctgcag taaaagttgc
    23701 ctcccttcta gctcaagagg gtagagatga tttaagtctt agagttgcag tacaaccagg
    23761 tggttgctca ggccttcgct accagctcta tttcgatgat cgctcactcg atggcgacac
    23821 ggtaatgaca tacggaagcg tcaaggtagt caccgacaag atgagtactc catatctaat
    23881 gggagcaact attgatttcg ttgacaccat agagaagcag ggttttacaa tcgataatcc
    23941 aaatgctggc ggatcatgcg cctgcggcga ttcctttaac taagaccgaa actaatttct
    24001 taaggctatg cctattgtgg gctggcttta gagttattcg ataatttccg ttttcgctat
    24061 atcccatagc tgttaagaca tggctagggg agagcggtcc tgagccgcag gcagaacctg
    24121 catcaattaa taccccttgg gcttcaactt tgcgtaacac ctcttcgcta tgagttcctg
    24181 gaataacaaa ggctagaaac cttggatcac aattattccc atctcctgca atctttatat
    24241 ctttaatctg gctagcaagc tcttgtctaa taaaggaatt tagttcagtt aatttaacaa
    24301 aatcttctct tgctctcttt gtccagttct ctagcgcaac agcagttgca agaagcagtg
    24361 gttttgaaaa agatccatag acccgtctct tatcgattat cggtcctggg ttttgccacc
    24421 tgccgctatt tgcaattcca actagagcaa ttccggaggg gccaccaaag tatttcggat
    24481 cccagattgc gctatcccaa ttatcgggaa ggtttgatag tggataggca gagctcatat
    24541 cagcaaaaac agcagtttta tctgaagttt ttggtgcgtt ttgaatcact ccagtttctc
    24601 tattaactgc ttgaaagcta agggttgcct ctctgctgct atctacttga aagtcaacaa
    24661 ttcctttgga atcaacgctc tgctcaagga cttctccgcc ccttgcttga tgttgccttg
    24721 caaaggcgtg gattacttgg cgatcaactc ttgagtagat gaatttggat ttgctctcag
    24781 ttaacagccc tgccaaagcg cattggaatc caaaacctaa ttcaccaact atctcaagct
    24841 cactacttgc aataccaaga tgagaggcga ttgattccct tgcttcattt atcaaatttc
    24901 ttaattgagc cgaatttcta tggattttcg caggatcaaa ccaagcagaa tcaaagagtt
    24961 caagtaaatg ttggcgcgcc ataggctcaa gaggcaactc actctgaaag tcatcctctg
    25021 gggcgatatc tgtgatgggc aacaccctta gaaacctacc ccctaatggg cgcagatgcg
    25081 aggcgcaagt attcttatcc catgcctgct cttaaaaaac ttgccctggc agcctctttg
    25141 ccgcttctgg ctggttgttc caatgaagag ctaactggaa ttggatatcg cgggggagtt
    25201 accagcgtca acgaggcatc ttttgcgctc tggcaatggg cttggctagc cgcggcagtt
    25261 gttggcgcct ttactctgat cttaattctc tggccagtat tttttcatcg caaaaaggaa
    25321 ggcgaagcat ttccaaggca atttcaatac aacattccag cagaggttgc ctataccgtt
    25381 attccattca ttatcgtggc tgttctcttt ggatacacag caaaggcaga gaatgaaatt
    25441 gttgcgctat ctccacagag cagcactgag cttcacgatg taacagttaa cggctttcaa
    25501 tggtcatggc aattcaccta taaagaggct ggcgaaggaa ccacagttac tggaacacct
    25561 ggccaaccgc cggtgcttta tatgccgcta ggtgagaagg ttagatttac ttttaccgca
    25621 tctgatgtga ttcactcatt ttgggttcct gatttcatga ttcagatgca gaacattcct
    25681 ggagtgacta atcagttgga atttactgcc aatgagttag gcgagtatcg gggattttgc
    25741 aatatcttat gtggaaggca gcactcgcag atgaggttta ccgtaaaggt tgttacaccg
    25801 agtgagtatg actcctatat ccaatccctt aaagctgagg tgaactcgtg accacttatg
    25861 ccactcgcac tctagcctca gatgagatgc cagaggctcc taagccaatg agtaagggca
    25921 aacttgcagt caagatatta acctcaactg atcacaaagt aatcggttat ctctatctag
    25981 gaacatcttt tgcttggttc ctaatcggcg ggatcttggc acttctttta agagctgagc
    26041 taactcgacc aggcatgcag tttttaagct ctgagcaata taaccaagtc tttactatgc
    26101 atggcaccat catgttattg atgtttgcaa ccccgctctt tgttggtttc gcaaatgtga
    26161 tcatgccact gcaaattgga gctgccgatg tggcatttcc aagaatgaac atgctctctt
    26221 actggctcta tctatttggt ggaattgttg cctttgcagg ctttctaacc cctgggggag
    26281 cagcaagctt tggatggact gcatatgccc cgttatcaaa tgatatttat tcaccaggaa
    26341 ttggcgcaga tctctggatt atgggtcttg ctgttagtgg acttggaaca attcttggcg
    26401 gagttaactt tgtaacaaca attttcacca tgcgcgctcc aggaatgacg atgtttagaa
    26461 tgtcgatttt ctcttggaac gttctactaa cttctatctt ggtgctaatt gccttcccac
    26521 cacttgctgc agcgctactt gcacttgaat cagatcgact atttggttca ctcatctttg
    26581 atcctgcaaa tggcggagcg atgctctggc aacatctctt ctggttcttt ggccaccccg
    26641 aggtttatat cttggcgctg ccattttttg gaattgcgag tgaaattttc ccagtattta
    26701 gcagaaaacc tatctttgga tataaaggcc tagtagcagc aacgattgcc atcgctgctc
    26761 tatctgttgc ggtttgggct caccatatgt acacctcagg tcaagtctta ctaccgttct
    26821 tctcatttat gactttcttg attgctgtgc caacgggggt gaaattcttt aattggattg
    26881 gaactatgtg gcgaggttct ctcacctttg aaactccaat gatctgggtt attggtttct
    26941 tggttacctt ccttcttggc ggcttaactg gcgttatttt ggctagcccg ccgcttgatt
    27001 ttgcagtctc tgcctcctac tttgtcgtag cccatttcca ttatgttctc ttcggaactg
    27061 tggtctttgc gatgtttgcc ggattttatt tctggtggcc aaagatgacg ggtcgaatgc
    27121 tcaatgagac gcttggaaaa atccacttct ggaccctctt cttaggtttt cacactacat
    27181 ttctaattca acactggcta ggaattaagg gctttccacg taggtatgca gattatctag
    27241 ccactgatgg ttttacctgg atgaatatgg tttcaacact tggttcaacc ctgctagcta
    27301 tatctatgat tccatttgct ataaatgtct ggataactcg caaatctcca aaagttgaaa
    27361 gcaatgatcc atggggctat ggctcatctc ttgagtgggc tacaacttgt ccgccgccaa
    27421 gacataactt ccttcgcatg cctgagatta gatctgagcg tccagctttt gatcttaatc
    27481 atccaaatca ctccaagatt gaaggccact aaatgaaggt tggctatcgc ttatttctat
    27541 tcctttttgt tttctatgca atcgtcggag gggtctactg gtatttaggg ggagagccgt
    27601 taggaattac tgccgtccta ttatgcgcag gccttgctct cattattggc tactacttct
    27661 ggtttatcga tagaagatct ggtggagttc ttccagaaga taataatgat ggacttatct
    27721 cagatggagc aggtgagctt ggtttctata gcccacattc ttggtggcca cttccagtag
    27781 gacttgctgc cactgctgca ggagtgggat taattgtggg atggtggtta acgttaattg
    27841 caattggcgc gctactagtt tcggttatgg gatttgtctt agagtatgaa aaaccatctg
    27901 cttcaagcca ctgatggaga aaatcagagc agaaatagtc gatactggca ttgagctttt
    27961 ctataaaacg cagttagaga ttaaagcgcc agccaaagta atttttgatc tcattgctga
    28021 tcccaaaaat catcaaaaaa tcgatggctc agggatgtta aagggtgaac tcaaagctcc
    28081 agatcggctc tatctcggag ctagctttgg gatgaagatg aaatatgtta ttccctacat
    28141 aattaagaat catgtagttg agtttgaaga gaataaatct ctcgcctggc gccatctgat
    28201 gcataacgtc tggcgctatg agctagtaga gctagatcca agaactactc gcgtgatcca
    28261 aacctgggat ggcagaaaag caagatctaa atggtgggta agtgattcct atcagtgggt
    28321 accaagggcg atggctaaaa ccctggttag attaaaggaa atggcagaga agcacgccag
    28381 ctaaacaaaa gcaaaaacta gtggtgtccg ccttcaagct ctttaacatc atttgctgtt
    28441 ggcttggcaa tctgctctgc ttgagagcga gatagacgag agcgaatctt tgaacggatt
    28501 gcctttggat ttctaacccc atactcatta cttaggtttg cctcaagcgc tggcagctgc
    28561 tcatgttggg ttagaaggta ggccttctct ggagttaagg gttcatgcac ttcaatgaat
    28621 tcaccgtgag gcagcattac aagtcgacca gtttccttgc cgtggagcac aagctcacga
    28681 tcagctctct gtagtgagag acagattcgc ttggtaataa caaaggcaag tggcggcaga
    28741 acaattacgc cgatacgaga gaaccacata atctgattaa tagagagatc aaagtgagtt
    28801 gcaagtaggt cattaccgcc gttaattagc gtgaccaaca taaaggtaat agacattgcg
    28861 ccaagagcag ttctatttgg agcatttctt ggacgatcta gtaggtggtg ctcacgctta
    28921 tctccagtta tccaactttc aataaatgga tagagcgcca ttccagtgaa gataattcct
    28981 ggaattatta aacctggaat caaaatattt aatgagagcg tgtagccaaa gatatatgcc
    29041 tcaagcggtg gagccatacg aaccaagcca tcaagccatc ccatatacca atcgggctgc
    29101 gagcctgcgc cgatttgtcc cgggttataa ggcccataga tccaaattgg attaatcgat
    29161 gccacagcgc caagaaatgc tgttacgcca aagacgataa agaagaagcc accagcctta
    29221 gccatatagg taggcattag gggatagcca acaacgttct tctcagttct gcctgggcct
    29281 ggatattgag tgtgcttctg ataccagacc agcattaagt gcacggtaat aagagctagg
    29341 aagattcctg gcagcaagag aatatgaacc acatagattc tctggataaa catctcacct
    29401 gggaaggctc cgccaaatat gaagaacgat agatatgaac ccacaactgg gatagcttga
    29461 ataattgctt ctgcaattct gattccagtt ccagagagaa gatcatctgg aagtgagtag
    29521 ccaaggaatc cttcaacaat tccaagggtt aatagtccaa ctccaataat ccagttaaat
    29581 tctcttggtt ttctaaatgc tccagtgaag aaaacacgca ttaagtgagc aaccattgct
    29641 gccataaaaa taagtgctgc ccagtgatgg atttggcgca ttaatagccc gcctcgaaca
    29701 tcaaatgaga tgtgaaggga ggaggcatag gcggcgctca tcttcaatcc agagagcggt
    29761 tgataggagc cttcatactc aacttcgcgc attgatgggt cataccagaa agttaaaaat
    29821 gttccggaga gcaataaaat aataaaagag tagagaacaa tctcaccgag cataaatgac
    29881 cagtgatctg ggaaaacctt agtaagagtt ttcttcagcc acttagcaga gccagtacgt
    29941 tcatcgacgt aattggcaac ttggccagca actctctctc ttgcgctcat tacttattcc
    30001 gcccccagaa acttggtcca actggttcac taaatggctg ctttgccact agatagcctt
    30061 ggctatcaac tgtgatggct aattgtggca ggggtcgggc agatggtcca aagataactt
    30121 ttgcagctct tgtcacatca aaggttgatt gatggcatgg gcagagaaga tgctttgtag
    30181 tctgttcata gagagctacc gcacatccca tatgtgaaca gatctttgaa aaagcgatga
    30241 ttccttcata agtccatgaa aggcgctcgg catccaagtt gaattcttca gggcggatac
    30301 ggatcaaaag aacagaatcc tttgcgatgt catctaagtg gcgcactttc ccggcaggta
    30361 gttcaggttg aacttgagca acgccgccaa cttcaagatc tgaggggcga atcgggcgat
    30421 ctcctgggtc tgtgacaagc ctggtgccag atttccaatt agtctctttc tgatcattac
    30481 ctggaagtgg tccaaggtct ccaagaagca gaacaggaga gatgccaact aaaccaagtg
    30541 agagcagcat cgttcgctta ataaaggggc ggcgaccaag tcctgcctca tttgcgcgat
    30601 ccttaactgt tgaaacaaag tcctcgcgat cttcatcttt ggagcgctgt tctttgcggt
    30661 aatcaattac ctcgtgatct ggcataagag tctttgccca atgcactgcc gccatcccaa
    30721 tgaagaagag cgccattgca agaccaaggc ctaaaaatag ttgatgggca tttgtgcttc
    30781 ctagtacggg aacgaaaata aaagcatctt catcaattcc taagtaagcg tatacaaaga
    30841 gcaaagttcc taaaactgag aagagaaata aaattccaac ttgtcgctct gcttttttag
    30901 cagctctaac atcaacatct gctttgcgat ggatgtgctc tggaagtcct gggtctgaaa
    30961 gtggggctaa atctttgctc atctaactac cgagcctttg ctgttagcca tactgccacg
    31021 ccaattaata gaccaagacc taatacccaa ccaactagac cttcagtgac tgggccaacg
    31081 cgaccaagtg ctgcgccgcc aagttgtggt tcagtctcag catgtttgat ccacttgata
    31141 attgatagtt tttcttctgg ggtcaaaata gtttcagaga aaacaggcat agcttgaggg
    31201 ccagtaatca tcgcttcata tatatgtttg acttcaactc ccataaccgt tggagcatat
    31261 tttccttgag tgagcgcgcc accttgtcca gctaggttat gacacattgc gcagttattt
    31321 ctaaatagct cgccgccttc tgccatgttt ccatcgcgct cccaagcaag ctcttcattg
    31381 gtgacagcag ctgggccagg ggctagagat gctacataag cagcaagagc tgctacctct
    31441 tgatcgttat aaaccggctc cttacgagca atttgttgac tcatatcagc aactggcata
    31501 cgacctgttg ctacttgaaa atcaacagag gcagctccaa cgccaattag tgatgggcca
    31561 actccagcgc cctctgcatt taagccatga caagaagagc agccacgagc aaatatttcc
    31621 ttaccctctg caattaaagt ggccttatca gattgggaga gcgttgcctg ctctgttaca
    31681 gcttgcgcag cgctataggt aagtccggca aagagcaaag cgccaagaag aagaagcgga
    31741 ccagcaaatc tatggcgtcg atactttgaa ataaatctca tctatgccac cagataaatt
    31801 gctgaaaaga gagcaatcca aacaatgtca acaaagtgcc agtagtaaga aacaacaatt
    31861 gcggtagttg cctgtgagtg gccaaagcga cgtgcctttg agactcgaat cattactatc
    31921 aaaaatgcaa tcagaccacc tgttacgtga agaccatgga atcccgttgc taaataaaat
    31981 actgaaccat aagcagcaga ggaaatagtt aaaccttcat gaaccagttc tgcatattca
    32041 taaacttgac cgccaataaa aaatgcgccc ataacgaatg tcagagcaaa ccactcccgc
    32101 attccccatt ttgaaagttg cagtagcgag cccgtgcgag cattttgaaa acgctcagct
    32161 gcaaagacgc cgaactggca ggtgaccgag gagagaacta agacagtggt gttaagggcg
    32221 gagaatggaa tattcaacat ctccacgccc tgaccccaga taacagggcc ttgcacagat
    32281 cgagttgtga aatacatagc aaagagagcg gcgaagaaca tcaactcact tgagagccag
    32341 acgatggttc caacagcaac gatatttggg cgattcaatc taggtggtgt ggagaccgcg
    32401 ctactcatag gggggattat tcctgaaatg agggcaatac cctctctttg aaggggtaaa
    32461 taagataggt atagcggtga aacgcatcac gggatattgg ctcagattga gcgaagggat
    32521 aagtcagtaa acccctagaa ttaccttaat tatggagacc agccagaaaa ccctaaaaat
    32581 cgcgctttac tcagatgatt caagcgttcg atctagcgta ataaccgctc tgggtaagca
    32641 aatctctcca gatttaggcg ctcataagat tttggaattt gctaccgcta aggccttacg
    32701 aatggctgtc gattctggtg cgaattttga tctcttcatt ctcgatggcg aagcagtgcc
    32761 tgaaggcggg ctgggagttg caaaacaatt aaaggatgaa gtctttaatt gcggcgtagt
    32821 tatcgtcttg gtggcacgaa gttctgatgc ctggcttgca ggttggtcta gagcagaggg
    32881 agtcattact catccgattg atccctttac tcttgcaaaa aatgttgcag agattcttgc
    32941 aaaaaaccca gttgcttctt aagtaagagc aagagcgatc atggaactca ccgtttcttg
    33001 ggatgagata gttgagaaat taacagctaa gcaagattta tctgacctgc aaattacctg
    33061 ggcaatggcg cagattcttg atggccttgc aactaatgat caaataaaga gttttcttct
    33121 tctgctaaag gcaaaaggcg agaagcctag tgaagttaga gccctcgttg atgtgatgta
    33181 tcaaagaggg attgcaattg atatccctga tcgcgctgtc gacattgtgg gaactggggg
    33241 agatggcgca tctactatca atatctcaac aactgctgcc atcatcacaa ctgctgcagg
    33301 cgcaagaaca attaagcatg gcaaccgcgc agctagttca aagagtggag cagcagatct
    33361 actagaggct cttggaattg atatctcact cggggctaag caaatccagg agtgtgtaag
    33421 aaaaattgga attgggtttg catttgcccc ggtcttccac cctgcaatga agaatgccgc
    33481 cagtgcaagg aaagaactag cgactcccac agtttttaac attcttggcc cacttgccaa
    33541 tcccgcaaag ccaaaggcgg tggcaattgg cgttgctaga gctgagttat tagagcttgt
    33601 tgcaacagtt ctcgccgagc gagattgcga gggttttatc tttaggggcg atgatggctt
    33661 agatgaaata tcactgagta caaccagtac ggtttatcag ataaacaaag gcgagataag
    33721 caaggagatt tttgatccaa gcgagctggg cctggaacga atcggtatag atgagttaag
    33781 aggcggtaca cctagccaga atgcagatat cacaagagct atttttgctg gcaaaggcgg
    33841 tccttatcgc caagcagttt tacttaatgc cgcagttgca attgctgcat ttaaggctga
    33901 ttttcatcta ggtattactc agcaatttgc taatggatat accttggcaa aacaagccgt
    33961 tgattctgga gcagcggctc aattagttga gcgctgggca aacctatcta ccgaattagc
    34021 aaaatcttcc ttataactat ttgcgccaac ttgagctcta accttttcaa tctctgatct
    34081 tgctgcagct gagccaaaag ttggcagggc ccagatgcca tcgatttcga caattcgaat
    34141 tccctcttga ttcaaataac gaagcagaac ctctacttct tcatgatgac tggcgggcaa
    34201 tatttcgcca ttatcgatta ctacctcagc agttaatttc aaggattgca caacctcctt
    34261 gtaattatca ctctttgcgg tagcgctcgc agctagtttg ccatggcgaa taacaacaaa
    34321 ttcccaagta tcaatggagg tagcaatcgc tgcgattaac tccttgactc tagttagact
    34381 tctaatgctc tgcgctctag cacttcctct aataagcgct cctaaacgat ttcgcacctc
    34441 agcagcctct tcaaaacgct cttggtttgc tagttccttc atccgctcgt taagcgagtt
    34501 aagaatgggg cgagagtcga tatgcaagct ctgatgaatc gctgttacat gtgatgaata
    34561 tgaatcaatt gattcctggc caatacaggg agccccgcaa cgcttcatat cgaataaggc
    34621 acagggacta gcgtttttca tggaagttaa cgtaattctt ggtttgcatt gtcttatctg
    34681 agtaacttca tagagcgcct ctattgctcg cgctgattca tctcggccat taaagggtcc
    34741 tgcccaaccg ctctcatcat caagcccctt tgcccctctt atggcaacta accttggaaa
    34801 agcttctttg gttagcttga tccataaaga ctttgcttga aatttggaac ggcgattaaa
    34861 gcgaggctgc ttctcactta tcagccttag ctctcttacc tctgcttcga taatcgttgc
    34921 ggtttgaatc acatcaagag agtgggcaag tgaaatcatg tcgtgaattc tttttcgagt
    34981 ctcagcagcg gtgaaatagg agcgaactct atttctcaaa tttcttgttg ttccgatgta
    35041 aagcggtatt cctgcttcat cccgaaagat ataaacccca ggcgtatttg gaaggccctc
    35101 agctaaatac ttcttacttc tctgggcgct agtaatacga gcgctgaagg atttcaactc
    35161 atctaaagtt tttacgccat agcttcctag tcgatcaaga attccatgaa agacatcaac
    35221 ggttgcgcga gcatctgaga gcgctcggtg atctggatta gttgttgcgc caaagaaagt
    35281 tgagagagtg gagagtttgc aattcggagc ttcatccctt gttactacat atcttgcaat
    35341 ttttgctgta tctaacactt gataagaagg ccaagaaatt tcaagctttg cacaagcagc
    35401 ctttagaaag ccaatatcaa agggagcgtt atgagccacc agaacgctat ctttacttga
    35461 gccaacaaat tctaaaaacg agaatattgc ttcgcctaat tttggcgctg caataaccat
    35521 tgcatcagta atgccggtta gcactgtaat gaaaggcgga atgggacttt ctgggtttac
    35581 cagagtctga aattcgccaa ctatttcacc cgccctaact ttcactgcgc caatttcggt
    35641 aattgcggcg ctgttatgag agccgccggt agtctctagg tcaacaacta caaaagtggt
    35701 ttcataaagg ctcggtgaga tctcatcaaa acttggctgg aaagcggaat taaattggct
    35761 ctcctgctca gatttcatgg agcaaagata gatgggatta atgacattta gcctgattaa
    35821 ctttagggtg cgcatatggc cattgaagga gcgcccccag gttggttatc tgactatcgt
    35881 gccaagggct ccggttcaaa tgctgatgtt ggagttcttc tagtccatgg cttcactgga
    35941 tctccagcat cgatgcgacc tttcgcgcat tttctcaacg aaaggggata tcaagtctct
    36001 gttcctgtaa tccccggaca tgcaagtaga tggcaggatc taaataaagt gcactggtct
    36061 agttggcccc aaaaagtcga gcaggaactt atcgagttaa agaaaacttg taggaaagta
    36121 tttatcgttg gcttatcaat gggcggtgga aatagtctct atgttgcagc agatcaccaa
    36181 gataaagttg atggaattgt cctggtaaat ccaatgattc atatcccagg tttacaaatc
    36241 aaatttgttt ccattatttc caaggtgcaa aagtctcgtg cctcggtagg agatgatatt
    36301 aagaaaccag gcgtaactga gtggggttat gacgccctac ctatcaaggg agtagcccag
    36361 ctatataagt atttgaagct ggctagaaag cgcctacctg gagttaaaac gccagtatta
    36421 ctttttcata gcgttgatga tcacaccctt gatgtttcca acactgagat aattctaaat
    36481 gagattggtt caagtgataa gcagagaatt gaacttctaa atagctacca cgtagcaact
    36541 cttgattatg atgcagacat tatcttcgaa aatactcgtc tatttatcca agaaagaagc
    36601 aaatgagcca aaacccattc ctaactaagt caaaccttga gtatgaattg ccgccatttg
    36661 cagaaatctc agaagaggat tatctgccag ctttctatgc cggaatggat gaacagctag
    36721 cagaggttga aaagatcact gaccaagctg aagtgacctt tgaaaatacc ttggtagccc
    36781 ttgaggcaag tggcgggatt ttaagtagag tcgctgcagt tttttacaat aaatcttcaa
    36841 gtgataccaa tgatgcgata gataaaatag aagcagagat cgctccaaag cttgcagctc
    36901 actccgacaa gatcaagtta aaccagaagc tctttgctcg aattacgcaa cttgtgaact
    36961 ctggacttga tttaaatagt gaggatgctt ggctattaaa gagatatcac cgcgactttc
    37021 tccatgcagg cgcgggactt tcagattcgc agcgcgagga aattaagcgg attaatgagg
    37081 agttatcaac tcttcagacc gaatttagta aaagagtctt gagtgaaacc aatgatctag
    37141 ctgtctttgt cgatgatatt aaagagctag atggcctttc agaaaatcaa attaaggcct
    37201 gcaaggcagc ggctgaggcg cgtggacttc aagataaata catgattggc gccgttaatt
    37261 tcactggcca tcctctactg cgctcgctaa agaatcgaga gctaagaaaa aagattatgc
    37321 aggcctcgct tagtaaaggt cttcgtggaa atgactttga tactaggcaa gttctactaa
    37381 agatggtcaa gttaagggcg aagcgagctg aaatttttgg aaagaaaagt catgcccagt
    37441 attcgttatc tgagcagact gccggctctc cagagagagt ccatgacatg ctcaaaaaga
    37501 ttgctccagc tgctcttgca aatgctaaag ccgaaggtag agatcttgaa cggctagcca
    37561 agctagatgg cattgaaaaa ctagagagtt gggattggga ttactacacc gaaaaggtta
    37621 gggctgagaa atacgacctt gatactgcgt taatgaagcc atattttgaa ctcgagagcg
    37681 ttttatttaa aggtgtcttt tacgctgcag gcaagttata tggcatgagc tttaaagagc
    37741 gcccagatct tgttacatat catccagagg cgcgggcctt tgaagtcttt aatgaagatg
    37801 gctcaaaaat tggcctctac attgctgatt tttatactag agattcaaag cgtggtggcg
    37861 cttggatgaa ctcattggta aagcagagcc acctacttaa tcagctcccc gttgtggtaa
    37921 ataacttaaa tatcccaaag ccaccaaagg gtgaggcaac acttctaact cttgatgaaa
    37981 caacaactct cttccatgaa tttggccatg ccatccatgg acttctctct aatgtgaaat
    38041 atcccaggtt ttccggaact gcggtgcaac gtgattttgt cgagtttcca tcgcaggtta
    38101 atgagatgtg gattct
//
LOCUS       DAOROD010000009        37823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_28_length_37823_cov_38.334145, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000009 DAOROD010000000
VERSION     DAOROD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 37823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37823
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_37823_cov_38.334145"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1781)
                     /locus_tag="PLW53_03180"
     CDS             complement(153..1781)
                     /locus_tag="PLW53_03180"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRD36458.1"
                     /translation="MRLRAPLPGWRAQADVVVIGSGVAGLTTALQIRAHNLSVLLVTK
                     SSIDAGSTKWAQGGIAAALGPGDSPSQHEEDTLVAGAGLCDLAAVKVLVSEGPEAVRK
                     LIARGAVFDKSETGEIALTREGGHLRNRILHAGGDATGAEVSRALLAAVSDDLGIEVI
                     EHALVLDVLKSNSGRVCGVTLHVIGEGSRDGVGEVLAKAVVLATGGLGQVYSQTTNPS
                     VSTGDGVALALRAGAEVSDVEFIQFHPTVLYIGEGSKGQQPLISEALRGEGAFLVNNR
                     GERFMRDVHKLADLAPRDVVAKEISRQMEITQSQHVWLDARGIKEFSERFPTIYASCR
                     EIGIDPANDLIPVSPASHYASGGVRTDLNGHSSVAGLYAVGETACTGAHGANRLASNS
                     LLEGLVFGARIAEDIAANQLPQEQPVAQDSYDFLLSPSIRGELQRAMSKGAGVVRSEA
                     SLKETLKTLERLKGAATIYASVDAWETTNLYLLATAIARSALERCESRGSHYRQDYPE
                     SANAWLKRITQKLASDGTWSSTITEVNSDLTIVK"
     gene            complement(1778..2587)
                     /gene="panC"
                     /locus_tag="PLW53_03185"
     CDS             complement(1778..2587)
                     /gene="panC"
                     /locus_tag="PLW53_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRD36459.1"
                     /translation="MKEVEVLSDLNNACAFVPTMGALHAGHQSLIRRARELADEVVVS
                     VFVNPLQFENKEDLAKYPTTPKHDSEFASEAGATILWRPSYESIYPGELKKLSSGEIG
                     RSFEGVNRPGHFDGVLTVVNRLFELCRPKYAIFGEKDFQQLFLIKEMAARLHPEIQII
                     AAPTIRDTSGLALSSRNVRLSSQGLESAEVISKVLRAASSESSLNSARQALKQIEKYG
                     SFTLDYAEIIDEESFKVADESSLKTRVIIAGWIEGVRLIDNMAMQNSLVGA"
     gene            complement(2642..3091)
                     /gene="folK"
                     /locus_tag="PLW53_03190"
     CDS             complement(2642..3091)
                     /gene="folK"
                     /locus_tag="PLW53_03190"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRD36460.1"
                     /translation="MRVVIALGANLGEPRAQLEEAIEALRKVIKIEAISSFYETKPFK
                     VNEEQPNYINAVLIGETNLEAEQLMKELLAIEERIGRKRSTINAARLIDLDIIDYQGI
                     AINSENLTLPHPRAFEREFVLRPWIEIDPEAQLTGFGAVKELLAALN"
     gene            complement(3088..3450)
                     /gene="folB"
                     /locus_tag="PLW53_03195"
     CDS             complement(3088..3450)
                     /gene="folB"
                     /locus_tag="PLW53_03195"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRD36461.1"
                     /translation="MSDKILITGIQALGYHGLLEDERKNGQLFVVDAEVELDLSEAAK
                     NDDIKKSVDYNALAILIHSEITGPAVNLIESLAHRIGIKALETFKEIDEIKVTVHKPK
                     APIEVSFTDVSVSIKVKR"
     gene            complement(3447..4256)
                     /gene="folP"
                     /locus_tag="PLW53_03200"
     CDS             complement(3447..4256)
                     /gene="folP"
                     /locus_tag="PLW53_03200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRD36462.1"
                     /translation="MSTLVMGILNITPDSFADGGMHFRIEDALKRADEMIAEGVDIID
                     IGGESTKPGAERITEDEERKRVIPVISELVKKEVALSIDTTRSNIAKEAIKLGVAYVN
                     DVSGGLADEKMYKVIAENPKVQYIAMHWRAHSKNMQEHAKYADVVKEVKEELENRVES
                     AIAAGVNPDQLIIDPGIGFSKLAEHNWELLRNLDRLALLGYPILIGASRKRFLGELTG
                     SSNTEDREAASIAITAMVAKQNIWAVRTHSVKPHKDAIATVNSFAKGRVST"
     gene            complement(4256..4828)
                     /gene="folE"
                     /locus_tag="PLW53_03205"
     CDS             complement(4256..4828)
                     /gene="folE"
                     /locus_tag="PLW53_03205"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRD36463.1"
                     /translation="MSKSFDQDRAERAISELLAALGEDLERDGLRDTPKRVARALKEN
                     FQGLYQDPKDVLTTTFDLGHEELIIVKDIEVYSHCEHHLAPFFGVAHIGYIPGESGKV
                     TGLSKLARLVDLYARRPQVQERLTTQIADSMVEILQPGGVIVIIDCEHLCMSMRGVRK
                     SQARTVTSAVRGMLRDPATRAEAMSLITGK"
     gene            complement(4833..6860)
                     /gene="ftsH"
                     /locus_tag="PLW53_03210"
     CDS             complement(4833..6860)
                     /gene="ftsH"
                     /locus_tag="PLW53_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRD36464.1"
                     /translation="MADKKVKVKPSKLPAKSGKKAAPQKFFKRPLFWILVAIVGVTIF
                     GQISSGSNQFTKIDTSKAMAAITRGDVESANIVDRDQKIQLILKPGVAINGATKVEAS
                     YVTGQEPILIDLLTANQPAKEWDVTVPKTNILVSILFTLGPILLLAFLLLFFMSQSQG
                     GSRVFSFGRSRAKLHDNEVPQVTFADVAGADEAIEEFREIKDFLAEPKKYQEIGAKIP
                     KGVLLFGPPGTGKTLLARAVAGEAKVPFYSISGSDFVEMFVGVGAARVRDLFTQAKAN
                     APAIIFVDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFEAHEQVILIAATNRPD
                     ILDPALLRPGRFDRQIGVDRPDVKGREAIIKVHSKNKPLEKDLDLLAIARRTPGFTGA
                     DLANVLNEAALLTARENRKVISSTDVDEAIDRVMAGPQRKSRLMSEEERRVTAYHEGG
                     HALVAHALPHTDPVHKITILPRGRALGYTMVLPDEDKYSTTRNQMLDQLAYTMGGRAA
                     EELVFHDPTTGASNDIEKATTLARAMVTQYGMTEKLGAIKLGSGDAAPFMGRDYGHQR
                     DYSEELAATIDQEIKTLIEQAHQEAYEILIENRSILDALVEELLEKETLQKEEIAVIF
                     KHVASRPKRPAWTGSSARIPSRIPPVAIKPRANGKSDEGVKRKKKPSSKEV"
     gene            complement(6863..7414)
                     /gene="hpt"
                     /locus_tag="PLW53_03215"
     CDS             complement(6863..7414)
                     /gene="hpt"
                     /locus_tag="PLW53_03215"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRD36465.1"
                     /translation="MDLESVKGEIERVVVTDEQLQVRLKEIAAQIEADYKNQDLLLIG
                     VLKGAVMVMADLSRYINRHLDMDWMAVSSYGSGTKSSGVVRIMKDLDKDITGRNVLIV
                     EDIVDTGLTLDWLSANLKSRGAKSVEIMTVLRKPDSAQVEVAVKYVGFDIPKDFVIGY
                     GLDFNEKYRNLPFIAVLAKHMYE"
     gene            complement(7439..8416)
                     /gene="tilS"
                     /locus_tag="PLW53_03220"
     CDS             complement(7439..8416)
                     /gene="tilS"
                     /locus_tag="PLW53_03220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRD36466.1"
                     /translation="MAETTPLYQIRLAVRSELEDIAAGDSVLVAASGGADSSALAAAL
                     LLECKTKSIKVIALIIDHGLQKNSADVTHETKRTLTKIGYENIEIRRVTVEITDGLEA
                     SARRARYQALNDVANSHNAVAVFLGHTKDDQAETVLLGLARGSGSRSLSGMASRVDRY
                     RRPLLSITRAQTEAACEEAGIKFWQDPHNQSMEFTRVRVREVVLPTMEKEIGPGISDA
                     LARSAKLLRDDADALDYLSDEIFSKLEPASLEISKLESQPRAIRTRVLRKAIYLAGAP
                     QGSLSAEHIEPVEALITAWKGQGPISLPGGVTVARISGRLSLSKSGGQG"
     gene            complement(8436..9644)
                     /locus_tag="PLW53_03225"
     CDS             complement(8436..9644)
                     /locus_tag="PLW53_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD36467.1"
                     /translation="MRSKRARFIALSTSLLLLIAHSPAYGKTPKPTLAQIEAAKKAEA
                     EKKKLADAAAAKLSKANLTLKQLTAVANAARAKYEAAKRELAIATKEAESAAAAYVVA
                     VSEVKAAHDSIGRLATNAYIMGGGFTDLNSVLHSDGPQDLIDRLSTLDTLGNQNTQAL
                     FRFQAAEAAAAKAKKAADYAKAAQEVATEKVEETKIEADAARNAQQKEVDKLQKIQDQ
                     VLKELNAARKTRVTLEQQRQLALLEEANANIATTTNVYTKVWPDIGFKGRSTSRSTPE
                     MRAEAVAFAKKQVLARKPYVWGDEGPNAFDCSGLVYAAYRSAGLGWPNWDRLNSSLYF
                     SYTKHVPLSQLEPGDLLFYSYKGTVNTIHHISIYAGNGMMWEANSKDKGLLFSNIYSV
                     KGLMPYGGRV"
     gene            complement(9736..10593)
                     /locus_tag="PLW53_03230"
     CDS             complement(9736..10593)
                     /locus_tag="PLW53_03230"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRD36468.1"
                     /translation="MISPYIALMKLRVVELLLVTTLPALFLAADGVPPLGISLATLLG
                     GTLAAGAANAFNMIIESDIDQLMDRTSKRPIVNKELSENQAFAFATALTVLSLSIFWI
                     FTNPLATALTIGAIIFYVFGYTVGLKRRTSQNIVWGGIAGCMPVLIGWAAVANSLSAT
                     AFLFFMVIFFWTPPHFWALAIKYKDDYEAAGIPMLPVVAPIAKVEQQMWFHTIAMFAT
                     SYLTLYSAGLPNWSYFANTVLTIFFAVQMFSLRSSQIEKSAAKIFHGSITYLSLYSIL
                     LVAAIFITF"
     gene            10623..11711
                     /locus_tag="PLW53_03235"
     CDS             10623..11711
                     /locus_tag="PLW53_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRD36469.1"
                     /translation="MRIYLAWAISLNLIFTSAIPTSAEPLSESFKLKLEATITGDISP
                     KSVRSSNTGFISAHNMMYRHSVTIYDAQSLELVKSISDQVNLKDLGIKGYSGLHRGSP
                     VEGAFSPDGKYLYVTNYAMYGKGFNREGTDKCNPGNNYDRSFLYRIDTQSWSIDAAYK
                     VGVVPKVVEVSPDNKYVLVSNWCSYDLSIISTELGKVVKTIKIGAYPRGITISNDSQY
                     AFVAQMGGSVVHKINLVSFERELLSVGSNPRALVLSPDNSYLYATLNAAGRVIAFDLA
                     TNKVVESVKTGSAARSLDISADGSALYVVNYTSDTLVKVRSSDFKILQKIKVCNEPIG
                     VTYERLQKRVWVACYGGSIKVFSDSPSQ"
     gene            complement(11689..12789)
                     /gene="dacB"
                     /locus_tag="PLW53_03240"
     CDS             complement(11689..12789)
                     /gene="dacB"
                     /locus_tag="PLW53_03240"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRD36470.1"
                     /translation="MAKSRVALLLALLLAINPLEASALHSLDKYVNSKHLATPGLLIL
                     NPLDGQVIAQNAPESLRVPASVLKLVSSTAALHFVGGERRYVTTIFTTEKENTYLIKG
                     SLDPWMTSNLSLAKKNGQKYLPSLISKANPENKKRLTIYYSSLFEKDRYDLNLNLKRK
                     GIKATFKKVDAAKAKEIAKEEIASLTSPPLSEMIKFVNLYSDNTLANRLAMAAAREIG
                     FERTSKGLTQTFKLALEEIGVNSEGLKAKDGSGLDKANRLSTQTVVELLVKIKDNPKY
                     QAIYEGLPVAGKSGTLKKRFIEDGPEAVGKVKAKTGWLRNTVTLAGYAKSADKEYVFA
                     IMADGITPTLSSRNKARAAMDRLLEAIVMGNH"
     gene            12840..13934
                     /locus_tag="PLW53_03245"
     CDS             12840..13934
                     /locus_tag="PLW53_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HRD36471.1"
                     /translation="MRTGYVYHEIFGWHDTGTFVGDMPSDPGAGLQPYMNYENPETKK
                     RIHELIVVSGLIEHLTRIPVRAASETELATVHTKEHIARIKKESASRLGGDGGDLTTP
                     FAKGGYEIAAMAAGGAIELFEAVLNGDVDNGYALIRPPGHHAVSETGMGYCIFSNLAV
                     AIAVAKKTHKKDLRVAVVDWDVHHGNGTESIFLNDTNVLTISLHQDQLYPHDTGSVDV
                     VGVGTNINIPLPPGTGHGGYYYAFDEVVIPAIKQFKPDIIAVASGFDSSVYDPLGRML
                     VTAEGYRVMTRKLMDVANQVCAGKLMMTHEGGYSASYAPFCGLFVLEELSGVKKLADP
                     FAHGNNYPGHELKEHEKRIVDQAKKLVSNL"
     gene            13931..14572
                     /locus_tag="PLW53_03250"
     CDS             13931..14572
                     /locus_tag="PLW53_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRD36472.1"
                     /translation="MSLPPGEIGPGEFYPVVGVGPHPKPWPSDEHFDPELLENGDRRN
                     VLDKYRYWKVEAIVSDLNTKRHSLRIAIENWQHDLNIGSVVRTANAFNVSSVHIVGKR
                     DWNRRGAMVTDKYLTVIHHATIAEFKAWADDHKVEIIGIDNLEISKPLESAKLPRECV
                     LFLGQEGAGMSDEAVAVCSQVLSIKQYGSTRSMNASAAGAIAMYAWAMQHLNK"
     gene            complement(14561..15064)
                     /locus_tag="PLW53_03255"
     CDS             complement(14561..15064)
                     /locus_tag="PLW53_03255"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRD36473.1"
                     /translation="MSAVDLSHLKGRDNLIITMVCYGNICRSPMAAAVLSNKVSKLER
                     PKIFVDSAGTSNWHVGQGPNPPSKRTWEKAGYSFEHIASQFNYSRLVAADLVLVMDKD
                     NHAEVSKYVTSDEEERKIYYLRQFDASRPDSLEVPDPYSLPDSAFENVLEMVEKATDG
                     LIKQLLI"
     gene            complement(15064..16002)
                     /locus_tag="PLW53_03260"
     CDS             complement(15064..16002)
                     /locus_tag="PLW53_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD36474.1"
                     /translation="MADEHNDDDVNDEIITEEMLWERIPETQGVDRASTYYELSARIY
                     ARGQYDEALALAETARDIYSELGSAAPSEGLAQAYSAIGYNLNQLKRMNEAATAMSKA
                     VELLRENKSPMALELACTLGEWWFSSKEYQNTIDCMQECAREHLIDGNELGAATDFHL
                     IGCAYRELKMYEKALEAFFEARKYFKDAKEVIHVARCDQKIAHCYTELGDADSALTAA
                     QKAVDVFTTAHDQRRLTYASFELGKAQVLSGEVYEGLTTLERVLDTAAEADLKDFEFI
                     VSIERRIAAILHTLGRSDEAIEIERRIKSVSEVIED"
     gene            complement(16085..17194)
                     /locus_tag="PLW53_03265"
     CDS             complement(16085..17194)
                     /locus_tag="PLW53_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD36475.1"
                     /translation="MKNLSTFSKVMVGFIAGAFVAGSVAVAVTPDMPPTKVCVDNRTK
                     ALYSATNGSCTKTRTLMEVAGSGVNVESIASAVSPSVVSIAVSATTGGGSGSGVIYRT
                     NASSSFIITNNHVIESAATSGSVRVELNNGDYEKATIVGRDSTYDIAVLKIDRGNLKV
                     ISVGNSSKLAIGEPVVAFGSPLGLSGTVTSGIVSALNRPVTTGSFGAESFIDAIQTDA
                     AINPGNSGGPLVDGTGALVGINSAIATLSSGTSGSIGLGFSIPINQAKRVVDEIIATG
                     KSSRPFLGVNFDTSYTGTGAKVLRLVPGEAADKAGIPAGAIIREIEGKKINDLITAIV
                     RVRSFAPGDTVKITVDMPGGGSKTFSVVLGRADSQ"
     gene            17271..18428
                     /locus_tag="PLW53_03270"
     CDS             17271..18428
                     /locus_tag="PLW53_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36476.1"
                     /translation="MDLALSRARIAVTVTFIINGFSAGSFVARIPDFKRILDISNGTL
                     GLSLLFVSAGVFLALKPAGKYSAKFGSQPVIFFSTIALALSYLLLGFLFSLAWFWITL
                     FIFGFVIATQDVSMNAHAVVVEQRAGRRLMSVFHAMFSVGTLFGGILGGVLSQLEITP
                     LSQASSLALIYIVAALLVRPLLLPASADTHHFGDEKRAKHPFIFAILGLFGLFAALSE
                     GAAGDWGGVLARETFGASPFISTLPYIVFCTAMIIGRLSGDYLAHRFGASKVIAAGGV
                     IAGTGLSAGLLIGGIPAIMVAWFLLGIGLSVVIPLMFSAAGTIALTKYSGVIAPSEAV
                     AKVSGVSYFGFVIGPPLIGFIADAFELRWTLMLLAGLSYLLILASRYARVA"
     gene            complement(18425..19864)
                     /locus_tag="PLW53_03275"
     CDS             complement(18425..19864)
                     /locus_tag="PLW53_03275"
                     /inference="COORDINATES: protein motif:HMM:NF005869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HRD36477.1"
                     /translation="MRLIHQPNHDLTYDDVFMIPSRSGVSSRMDVDLNTLDNTGATIP
                     LVVANMTAISGRRMAETVSRRGGLSVIPQDIPIDVVTEVISWMKSRDILFDTPITLTP
                     EQTVADAASLIHKRAHGAIVVVENNKPLGIVMEEDWEGADRFTQLSRIMSKDLMVIPS
                     SVSARQAFDLLTEKNRRLAPVVDASGNLIGIITKIGALRATLYKPALDNQGRLRIGAA
                     LGVNGDVKTKAAELIKAGVDLLVLDTAHGHQEKMIEALKAVRSLNPSIPIVAGNVVTA
                     EGVKDLIEAGADIVKVGVGPGAMCTTRMQTGVGRPQFSAVMECAEAARKLGKHIWADG
                     GIRHPRDVALALAAGASAVMVGSWFAGTYESPSDLRVDSSGRLYKESFGMASARAVAA
                     RTSNEDAFERARKALYEEGISTSRMYLDPIRPGVEDLIDEIIAGLRSSCTYAGAKNLS
                     EFAQKAVIGIQSASGYAEGRALNTSWSKS"
     gene            19908..20834
                     /locus_tag="PLW53_03280"
     CDS             19908..20834
                     /locus_tag="PLW53_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36478.1"
                     /translation="MSEALISARGLTKRYGDFTAVDAIDFDVAKGESFGLLGPNGAGK
                     STTMRIIAATSQRTSGTITILGRDPEEHGPQIRAHLGVVPQQDNLDTELTVTENLFIY
                     GRYFGLSKKFIRTKIEELLEFAQLEEKRDVKVDALSGGMKRRLTIARALVSEPDILML
                     DEPTTGLDPQARHILWDRLFRLKELGVTLVITTHFMDEAEQLCDRLVVMDKGKIMAEG
                     SPLELIKTYSTKEVLEVRFGSDRNKEIAPTLRAMCSRMEELPDRILMYVEDGEALLEE
                     ILNKKLHPTTSLVRRSSLEDVFLRLTGRTLIE"
     gene            20831..21643
                     /locus_tag="PLW53_03285"
     CDS             20831..21643
                     /locus_tag="PLW53_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36479.1"
                     /translation="MNSGINVAKVRSIGAIYVAEARMSAMLKWKGIIAIVAIGNPLFY
                     LIAIGIGVGVLVSENSGTSGTGGVEYITFIAPALLASAAITGALDEAMFPVMEGFKWR
                     KLFYAMNATPITGPQIALGVFIAALARVAFTVICYWAVLIAFGVMSVEQSWDVIPVTI
                     FAAGAFAAIIMGITAKVKNDDYFMTVLGRLIIMPMFLFSGTFFPLNTMPIYLQPIGWI
                     SPLWHATELGRYFSYGYSISAQMLIVHFAFLLALLIGGFALANRTFTTRLTK"
     gene            21640..22461
                     /locus_tag="PLW53_03290"
     CDS             21640..22461
                     /locus_tag="PLW53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36480.1"
                     /translation="MSNAVAIADARANRVGHQLYAGRARAIIERSLIALRSSTWVPVA
                     TGFVEPVLYLLAFGYGMGELIGNVTLGDKTIDYTVFIAPGLLATSAMNGAIYDSTWNV
                     FFKLNESKLYQGMLATPLGPLDVALGEIIWALFRGLSYAIAFLAITAPLGLVPGVSAV
                     LAIPAAVFIAFGFASFGMAITSYFKTYQQMGMINIVLLPLFLFSGSLYPISVYPDWLE
                     FLIQLMPSWHGIELVRDVWFGNFDSGTLLHISYFLVMIICGLYFTTRRLRDLFMR"
     gene            22599..23993
                     /locus_tag="PLW53_03295"
     CDS             22599..23993
                     /locus_tag="PLW53_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD36481.1"
                     /translation="MFNKTHHAETFRSTPYKTKPISAARINELLEIEKEKFAKLAPES
                     GVENRRASQVTPKGVASSFQHWDPYPISIVAAKGAWLTDVDGRQMLDLSMGFGALLVG
                     HLNEEVVKEAHATLDVGTLFATPSPITRSGAEIICRRFGLDMVRFANSGTEATMYALR
                     TARAATGKLGVIKIEGGYHGSYDPLMVSAKPEIDRAGDANSPNAVPLGATVPGDVWVV
                     PYNDAEAIERVLKKDADQIACVFMEPVLENIGIVLPDLGYLERVRELCDQYGVLLVFD
                     EVKTGLTAGPQGAAQRLGVKPDLIAIAKSIGGGIPVAAFGGKKEYMEVVVNGKHNHLG
                     TFNGHYLAMAGIRAVDRLCTPEALKRCEDLNRDALSKIAEIINEYEIPAHTVGFGVKG
                     CITWSASPVRNYRDYKATDFDIAELSFFWSLNRNVMTPPGLDEQWLISLAHGQREIDI
                     IVGDFLEFAKALRA"
     gene            complement(23990..24757)
                     /locus_tag="PLW53_03300"
     CDS             complement(23990..24757)
                     /locus_tag="PLW53_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRD36482.1"
                     /translation="MDENLTLSIDCGGLYLKSCVLDSSGTMHSKPNRIPTPYPLPPAT
                     FVETIAEIASGLPKAYRATIGMPGMIRNGVVISTPHYITKSGPRTKIDPESAELWSGF
                     DAQKAISTKLNVPTLVLNDAEVHGAGIITGSGYEVVITLGTGLGFAIFYGGKLTPHFE
                     LSHATVRRATTYDTWIGEQERKRLGTIFWSRRIRVLVEELRPVFKWDRLYLGGGNARL
                     IRPIDLERMGDDVVVVPNTAGVAGGVRAWNLEHYYRA"
     gene            24845..25867
                     /locus_tag="PLW53_03305"
     CDS             24845..25867
                     /locus_tag="PLW53_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36483.1"
                     /translation="MALIEIRDLAKSFGNVKAVDDISLDIESGEFITLLGPSGSGKTT
                     VLRMIAGFEDPDAGSIKLNGEDITHLPPFDRDVNTVFQDYALFPHMSVQENVEYGLRT
                     RKVPKAERAKQALEAIASVKLEETVNRLPHQLSGGQRQRIALARALVLRPRVLLLDEP
                     LGALDRQLREEMQVELKKIQRDAGITFVFVTHDQEEAMRMSDRIVVFNSGRIEQVGTP
                     EQVYEEPRTNFVAGFLGTANIFSVDVARKFLGATTTVSIRPERIRLQAPETKIDKSET
                     CVKATVQEAAFVGANTIYILETDFGVKLTVRKTNTELLGQDNSFVAGDQVMAVWRNSH
                     VAQIPV"
     gene            25898..27070
                     /locus_tag="PLW53_03310"
     CDS             25898..27070
                     /locus_tag="PLW53_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD36484.1"
                     /translation="MKGKKLAILSALSASVLIISGCSSGSDESSGVAIEVPDIPMHEA
                     MGEFEGELNIVNWSGFVEPAWTDKFTADTGCKVNPRVAGTSDEMVTLMRTGQYDIVSA
                     SGDAALRLIVGGDVQPLNLDLIPNFSDDIAAGMKGNIYDTVNGKPYGVPIGRGANLLQ
                     YNEDVTGGAPESWDVVWELNTPYKGKITAYDAPIYIADAAVYLMYHKPELGIKNPYAL
                     DETQLAAAIDLLKQQNQVIGEYWSDPVAQITSFVGGNTVVGTSWEVLRKFAAKDNLKT
                     TLPVEGSTGWFDSWLVSSTTKNINCAYAWMDYTSKPEVNGAIAVNFGMAPANVAFCAS
                     SPEAQAHCDTFFAEDEEFFKRIWPWTTPIETCVDGRKDVKCTSFQEWTNAWATVKG"
     gene            27126..28007
                     /locus_tag="PLW53_03315"
     CDS             27126..28007
                     /locus_tag="PLW53_03315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36485.1"
                     /translation="MSTKSDYRGSRPISSFFLRKPKVRLISLLSLPMTWIVGVYIVSL
                     FLLLITAFWNVDPFTSRVSPGFNLENFIMVFTVPAYIATSIRTLFIALSVTAICVLLA
                     VPLGIYMAKIAKPWLRNILAVAITLPLWAGYLVKIIAMRLVFTEEGFFNWLMAPLGIR
                     GPGFSIPIVILTLTYLWFPYMALPVYTAIRQIPPNLFDASSDMGAKGFTTVYRVVIPL
                     IVPAIIAGSVFTFSLSLGDYLAARFTGGATQMIGSIIAANINLNPPVAAAYSMIPIAF
                     VVIYLMVARRTGSLERM"
     gene            28089..28820
                     /locus_tag="PLW53_03320"
     CDS             28089..28820
                     /locus_tag="PLW53_03320"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36486.1"
                     /translation="MVLILNSFNESQISGWPIESFSTKWWEFARSYQPIRDALLNSVL
                     VATGAMVIASILGTLVAFAMKGFSFFGKGTVNILIVLPIALPGVVTGVAFSNTYSNFL
                     TPMGFNIGYFGMIVAHSTFCIVMVFNNVFARLRRMNPNLQEASADLGAGLWQTFRLVT
                     FPQFRTSFAAGALLAFALSFDEVVVTIFTAPPGVDTLPLWILKEVGRPNQASVVNVVA
                     TVVILLSLIPVYISQRLARAQEDER"
     gene            complement(28825..29502)
                     /locus_tag="PLW53_03325"
     CDS             complement(28825..29502)
                     /locus_tag="PLW53_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRD36487.1"
                     /translation="MKVFAHRGFSHKYPEMTQIAYRAAIEAGVDGFECDVRLTKDKEI
                     MCFHDSDTKRITGRRKLVSRVSSQELIRDANAIRLEELLVMAIEAKKDLLIESKHPVL
                     SSGEIEYRTMDLINKYRQGIDTAGIDVVIMSFSFWAVRRVDKRDAKCAKVIRYGISAL
                     FARYEYVAIKITTLKRYPFLINLMRAKRIYVWTVNLKEDLKWLKSREIYGVITDRPKR
                     ALRILKN"
     gene            complement(29499..30272)
                     /locus_tag="PLW53_03330"
     CDS             complement(29499..30272)
                     /locus_tag="PLW53_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD36488.1"
                     /translation="MNEVILTIAYSTLTKRLGNIKYPKSSPDQEIIVLVQNPDESNYN
                     TEKLQGKLVELKSRGVAKSRNAAIQYASGKYLIFGDDDITFVEEGLQECIAYFESHPD
                     CSIIMAQTSDENGKLRKAYPKKAHKLSRFNSAKAATYELMIRIESIRNAGIRFDENFG
                     AGADNYLGDEYIFIADSIKKGLKGVFLPIKLAIHPSESSGSAWGTQRDLGARAKVFSR
                     VFGPIAPFFRALFLIKSQLFKFRKGRVGILNSLRFIIGR"
     gene            complement(30265..31050)
                     /locus_tag="PLW53_03335"
     CDS             complement(30265..31050)
                     /locus_tag="PLW53_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36489.1"
                     /translation="MIAIAREMISGFRYLFPRGKFRIVLIALSALAATISVSELLVMK
                     FFATLVLNEEEFTREFLGWAIAGFFLFFILTRVSQYFQRSYRVKAFARSFRAAKKDRT
                     VKEENREWAMAFELSNILSFATQLVAIITFFYFLDPLLALINTLILIVVLQALGWIFN
                     KQVMVQKDLAIRNQKKRIKPEKRYSARIKAGESGALVSSAGTLVLLAALLYLSVDSQI
                     SPTNALIVFLGARLQNSVISNTSRSLMRYAKSSARIDSDDDLE"
     gene            complement(31047..31832)
                     /locus_tag="PLW53_03340"
     CDS             complement(31047..31832)
                     /locus_tag="PLW53_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD36490.1"
                     /translation="MIDRPKNPTIAQIREISQPASVTGRANAEHWVADLYLRKVSPYL
                     TRLLLKTPITANGVTYLMIATGLSISAALLIPGYQGILLALFLSQLQMLWDCCDGEIA
                     RWKNTQSPKGIFLDRVAHYLTESLIPIAFALRLSGWPENEISDWRLPFIAALISIAIL
                     LNKGFNDAVHVSRAFAGLSKLGDSKESGAPTKNWLRIIKLPFKIFAVQRLFHSVEMSI
                     VIAIFGNNPALIEIGIFVALFVTIGHLISILGSRKLDGMRETK"
     gene            complement(31832..33469)
                     /locus_tag="PLW53_03345"
     CDS             complement(31832..33469)
                     /locus_tag="PLW53_03345"
                     /inference="COORDINATES: protein motif:HMM:NF040266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5941 domain-containing protein"
                     /protein_id="HRD36491.1"
                     /translation="MDKSLTSLVVTSSTKVREILDSFKASNSDLLLLDLNSVIAQPHL
                     ELLTDYPRSASVALVSTQKNGDTLVRQNRIASASSSSHKVTIGNRNFAGAIRISQRQR
                     DEVIKALEGAANSELSGNAIDLVLVALTRARVQVDAAELWAAPYARSSDELVREKVKS
                     ELANLNMGKLRLKMANRANDGFYSVFFLRKFSKLLTWLAVRVNATPNQVTLISFAIGL
                     YSAFCFTKGTFSQTLLGAVLLQLSIIVDCVDGELARYTRKFSKLGAWLDAVTDRVKEY
                     MVFLGLAIGAEKSGQDLWIPALVMMLIQTFRHLSDYNFARSIKQRVEEQFLAPIDFSA
                     DFDGIIPSEREPKGRLRYWLGKIIQFPIGERWLVISASAVIGGASFTFTVMPILAFIS
                     ALVVFRGRTIRTLKMQKERSRGILISAQQDSLFVRLSFLKRFDWIEPSFLRVIEFAFL
                     LWIFIRNEQINMVSFVIFFAIVFHHYDNLYRSLQNEKKPIWLSSLGLFIEGRLLVLAI
                     AAFSGWSLNYFAWYFSTIFLVISSLQWVLSHRANKVG"
     gene            33488..34183
                     /locus_tag="PLW53_03350"
     CDS             33488..34183
                     /locus_tag="PLW53_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HRD36492.1"
                     /translation="MTLQVVILAAGMGTRLGKPWPKPLTPLADGRSIMAQQLENVEKV
                     FGQEARVTIVVGFKMEMIMEAHPNAAFIYNEVYDQTNTSKSLLKALRASQESGVLWLN
                     GDVVFDSRVLERVQSRMREEKSFVCVNTSATSDEEIKYTVDKDGNINSLSKQVKNALG
                     EAVGINFIGSGDKAHVITHLEECADNDYFERGLELAIEKAGIKLEPVDISDLFAVEVD
                     FQADLERANKGLV"
     gene            complement(34180..35301)
                     /locus_tag="PLW53_03355"
     CDS             complement(34180..35301)
                     /locus_tag="PLW53_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36493.1"
                     /translation="MSEPRLVIHAGFHKSGTTALQESFDSQSLELKQNGIVYPNIGRK
                     AHHRIAWALSQRMWGWNKKGGEKTSKKEWTRLVKAINADKSETVLLSSEFFSELDTEQ
                     IQTIFNEIKGRKIEALFTVRPLVKLLSSSYQQYLKYGITADYVTWLHSVLDKPGESKI
                     NPTFWMRHFHGQVVTKWAKVLGTGNVTVLIVDESKPEFLYDSINQYLGLPKDFIKPQK
                     IGSNRSLNLAEIALLIELNRQFPKDRTWNEYEIFIRDGYIRRLTDEIKSPSDSAKLLT
                     PKWAIDKGNEIGAQNKDELVASGATIIGDINTLDSASVPEGDPVYPTEIAIAHIAQAM
                     LAFDKDLIKKLPLSWIKKSLVKRYRRKLRVQISRTRNQI"
     gene            complement(35358..35966)
                     /locus_tag="PLW53_03360"
     CDS             complement(35358..35966)
                     /locus_tag="PLW53_03360"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HRD36494.1"
                     /translation="MSTGKKVLISGFEPFGGSDLNSSQLVVEEISKESFTGLELFTVI
                     LPVEFDKAAEVLLSKVKEINPEIIIAFGQAEGRRALTPEKIAINLDSARIPDNAGELR
                     VNKIIIEKGADGYFSTLPIEKMVGAVKECGLESEISLSAGAFLCNHIFYHLQHQLLES
                     RVKSGFVHLPLVNEQIAQYPNQPSWALKDLVQGVRAAILSTL"
     gene            complement(35985..36551)
                     /locus_tag="PLW53_03365"
     CDS             complement(35985..36551)
                     /locus_tag="PLW53_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRD36495.1"
                     /translation="MIKQLSSAWTLLFAGIIGFFASFILTIDKFKVLKDSGFTPSCNI
                     NATINCKSVMLSDQAEVFGFPNSLIGIATFAMMVVISVALFLGVRFPKLFWQLLVSGT
                     VLAAIFCHWLAFQTIFEIAALCPYCMVAWVATLLVLSVGLRELLELRKDSSKDEVDKA
                     AIETIAKWMLPLHILWATLLVGAAFLGV"
     gene            36629..36775
                     /locus_tag="PLW53_03370"
     CDS             36629..36775
                     /locus_tag="PLW53_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36496.1"
                     /translation="MYNDEDWKDDLREKKRLSDEEIHRNKRSWKKLSFIALLIIAVIG
                     VFKG"
     gene            complement(36772..36948)
                     /locus_tag="PLW53_03375"
     CDS             complement(36772..36948)
                     /locus_tag="PLW53_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36497.1"
                     /translation="MDSKERAIFDLAMVTIFIATVWASIELQWGVGVGIWVGGILSAA
                     LLLLYDLTSNWPDN"
     gene            37052..37381
                     /locus_tag="PLW53_03380"
     CDS             37052..37381
                     /locus_tag="PLW53_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD36498.1"
                     /translation="MRFMINVIESIEKPAHSRQEIDAINAFNDEIVAAGQRVLAVGIA
                     SPDNSILIDNRDGDIKYINESFNGSSEYVSGIWIIDVPSREIALELAARGSKACNRRV
                     ELRPLLG"
     gene            37597..37815
                     /locus_tag="PLW53_03385"
     CDS             37597..37815
                     /locus_tag="PLW53_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36499.1"
                     /translation="MATAIFFILIFPGNIRQYVDGIDAFGLDTDRKRAIRLLFQPLLV
                     LWALWSTDALNKYILRARRDSNPKPSDP"
BASE COUNT        10309 a         8252 c         7864 g        11398 t
ORIGIN      
        1 cactctttct tgtaacaaag cgggcattac ttaattgaga ttggttaatt gttgcttgac
       61 tagttaaatc atcggtaatt gagtcttcag ataatgcttc acttaccttg ctattgattg
      121 catcaggatt taacccttga tcagcgatta acttacttaa cgattgtgag atcactatta
      181 acctccgtaa tcgttgagct ccaagtgcca tcacttgcta gcttttgagt aattctcttt
      241 agccatgcat ttgcactctc tggataatcc tggcggtaat gagagcctct agattcacac
      301 ctttcaagtg ctgagcgagc aatcgcagtt gcaagaagat ataaattagt agtctcccaa
      361 gcatcaacgc tggcataaat tgttgcagcg ccttttagtc gctctaaagt ttttaaagtt
      421 tcctttagtg atgcctcact tcttactact ccagccccct ttgacatagc tctctgtagc
      481 tcgccgcgaa tagatggaga gaggagaaag tcataggaat cctgagcaac gggctgttct
      541 tgaggtagtt ggtttgcagc aatatcttca gcaattcgag ctccaaatac caagccttcc
      601 aataaagagt ttgaagcaag gcgattagcc ccatgcgccc cagtgcaagc agtttcacca
      661 actgcataaa ggccagcaac tgagctatga ccatttagat ctgttcttac accaccagag
      721 gcatagtgcg aggcaggaga tactggaatc aaatcatttg ctggatcaat tccaatctct
      781 ctgcatgagg cataaattgt tgggaagcgc tctgagaatt ctttaattcc tcttgcatca
      841 agccagacat gttggctttg agtgatctcc atctggcgtg aaatttcttt agcaacaaca
      901 tcgcgaggag caagatcggc aagtttgtga acatcgcgca taaatcgctc gccgcgatta
      961 tttactagaa atgctccttc gcccctaaga gcttctgaaa ttagcggttg ctgacctttt
     1021 gaaccttcgc cgatatatag aactgttgga tgaaactgaa tgaattccac atcagagacc
     1081 tctgctcctg ctcttaaggc aagtgcaacg ccatcaccag ttgatactga tggattggta
     1141 gtttgagaat aaacctgacc cagcccgcca gttgctagca ctacagcttt tgctaacact
     1201 tcgccaacgc catctcgact accttcgcca ataacgtgca aggtaacgcc gcaaactctt
     1261 ccactattac tctttaatac atctaaaact aaagcatgct caattacttc gattccaaga
     1321 tcatcagaga ctgcagcaag gagagctctt gatacctcag cccccgttgc atctcctcca
     1381 gcatgaagga ttcgatttct taagtgccca ccttctctag ttagggcaat ctcgccagtt
     1441 tctgatttat caaagacagc gcctcttgca attaattttc ttaccgcctc tggcccttca
     1501 ctaactaata cttttactgc tgctagatcg catagccctg caccagcaac aagagtgtct
     1561 tcttcatgct ggcttggtga atctcctggt cctagagctg cagcaattcc accttgcgcc
     1621 cattttgttg agccagcatc aatggatgat ttagttacta gtaatactga aagattatgt
     1681 gctcgaatct gaagcgcagt agtaagtcct gcaacaccag agccaataac tacaacatcg
     1741 gcttgggctc tccagccggg aagtggcgct ctaagtctca tgcgcccacc agtgagttct
     1801 gcatcgccat attatcgatc aatctaacgc cctcaatcca cccagcaata attactcgag
     1861 ttttaagact tgactcatct gccactttaa agctctcttc atcaattatt tcagcgtaat
     1921 ccaaagtaaa tgaaccatac ttttctatct gcttcaaagc ttggcgcgcg ctattaagac
     1981 ttgattcgct gcttgccgct cttagtactt ttgaaataac ttcagcactt tcaaggccct
     2041 gactacttaa tctgacattt cttgatgaga gagcaaggcc actggtatct cggatagttg
     2101 gcgcagcaat tatctgaatc tctggatgga gccttgctgc catctccttt atgagaaaga
     2161 gttgctgaaa atctttctca ccgaagattg catattttgg tcggcataat tcaaagaggc
     2221 gattaactac ggttaatact ccatcaaagt gacctgggcg attaactcct tcaaagcttc
     2281 tgccaatctc acccgatgag agcttcttta attcacctgg ataaatactt tcataacttg
     2341 gacgccagag aattgttgct ccggcctcgc tggcaaactc gctatcgtgt tttggagtag
     2401 ttggatattt agctagatct tctttgtttt caaattgcag tggattaaca aaaacagata
     2461 cgacaacctc atctgctaat tcgcgggcgc gcctaattag tgattgatga ccagcgtgaa
     2521 gcgctcccat ggtgggaaca aatgcgcatg cgttattaag gtcgcttaag acttcaactt
     2581 ctttcacctt atttacagct ccagtccgtt agtggtacca ggcgatggca agtctactcc
     2641 gctaattaag ggctgccaaa agctccttga ccgctccaaa accagttaat tgcgcctctg
     2701 gatctatttc tatccaaggc cgcagcacaa actcccgctc aaaagccctg ggatgaggca
     2761 gagttaagtt ctcactattt attgctattc cttgataatc aataatatct agatcaatta
     2821 ggcgagccgc gttaatagtg cttcgctttc ttccaatgcg ctcttcaata gctagaagct
     2881 ctttcattaa ttgctcagct tctaaattag tttcaccaat taatactgca tttatgtagt
     2941 taggctgttc ttcattaact ttaaatggtt tagtttcata aaaggaagag attgcttcaa
     3001 tcttaattac ttttctaagc gcctctattg cctcttctaa ttgcgctcta ggctccccaa
     3061 ggtttgcgcc aagtgcaata actactctca tcgctttact tttatgctca ccgagacatc
     3121 tgtaaagctg acctctattg gcgctttagg tttatgaacc gtaactttta tctcatctat
     3181 ctccttaaac gtttccaaag ctttaatacc aattcgatga gccaagcttt caattagatt
     3241 tactgcaggg ccagttattt cgctatgaat caaaatagca agagcgttgt aatcaacact
     3301 ttttttaata tcgtcatttt ttgccgcttc agataaatca agctctacct ctgcatcaac
     3361 aacaaagagt tgaccgttct ttctctcatc ttccaatagg ccgtgatagc ccagagcctg
     3421 tatgcctgtt atcaaaatct tgtcgctcat gttgaaaccc tacctttcgc aaatgagtta
     3481 acagttgcga tggcatcttt atgaggctta accgaatgag tgcgaaccgc ccaaatgttc
     3541 tgctttgcaa ccattgcagt tattgcaatt gaagctgcct cccgatcctc agtattactt
     3601 gagccagtaa gttctcctaa aaatcgcttt cttgaggctc caattagtat cggatagccc
     3661 agaagtgcaa gtcgatcaag gtttctcagt aactcccaat tgtgctcggc tagctttgaa
     3721 aaaccgatac ctggatcaat tattaattga tcagggttaa caccagctgc aatagctgat
     3781 tcaactctat tttctagctc ttcttttact tccttaacta catcagcgta tttggcatgc
     3841 tcttgcatat ttttggagtg ggctctccaa tgcattgcaa tgtattgcac cttaggattt
     3901 tcagcaatta ccttatacat cttctcatct gctaaaccgc cacttacatc atttacatac
     3961 gcaacgccta atttgattgc ttctttagcg atattagatc tggtagtgtc aattgagaga
     4021 gctacttctt tctttactaa ctcacttatt actggaataa cgcgttttct ctcttcgtct
     4081 tcagtaattc gctctgctcc aggttttgtt gattccccgc caatatcaat tatgtctact
     4141 ccctcagcga tcatttcatc tgctcgcttt aaggcatctt ctatccgaaa gtgcatgcca
     4201 ccatcagcga aggaatcagg ggtgatgttt aaaatcccca ttactaaagt gctcattatt
     4261 taccagtaat taaactcatt gcctcagctc tagttgctgg atctcttaac atcccgcgaa
     4321 cagcgctagt gaccgttctt gcttgggatt ttctgactcc ccgcattgac atacatagat
     4381 gctcacaatc gataatgaca attaccccac ctggttgaag aatttcaacc attgaatctg
     4441 caatctgagt tgttagcctc tcttgcactt gaggtcgtct ggcatagaga tctacgagcc
     4501 tggctagctt tgatagtccg gttacttttc ctgattctcc tgggatgtaa ccaatatggg
     4561 ctacgccaaa aaagggcgct aggtgatgct cacaatgtga ataaacctca atatctttca
     4621 cgattatcag ctcttcatgg cctaaatcaa aggtggtagt taatacatct tttggatctt
     4681 gatagagccc ttgaaagttt tcctttagag ctcttgctac tcgctttgga gtatcgcgta
     4741 ggccatcgcg ttcaaggtct tcgccaagag ctgcaagtag ttcacttatt gctcgctcag
     4801 ctctatcctg atcaaaggat ttactcattt aactaaactt ctttcgaaga tggctttttc
     4861 tttcgtttta cgccttcatc acttttgcca tttgctctcg gctttattgc aaccggagga
     4921 attcgagatg gaatgcgtgc agacgatcca gtccaagcag gcctctttgg tcttgaagca
     4981 acgtgcttga aaattaccgc aatctcttcc ttctgcaagg tttccttttc taacagctct
     5041 tcaaccaaag catcaagaat cgaacggttt tcaatcaaga tttcataggc ttcttggtgg
     5101 gcttgttcaa ttaaggtttt aatctcttga tcaattgttg ctgctaactc ctctgaataa
     5161 tctctttgat gtccgtaatc tcttcccata aatggagcag catcaccgct tcctaactta
     5221 atagcgccta acttctcagt cattccatat tgagtgacca tggcgcgagc aagggtggtt
     5281 gctttctcaa tatcgttaga agctccagtg gttggatcat ggaataccag ttcttctgca
     5341 gctcttccgc ccattgtgta tgcaagttga tcaagcatct gatttctggt ggtggagtat
     5401 ttatcttcat cgggtaagac catggtgtat ccaagtgctc gccctctagg caaaattgtt
     5461 atcttatgaa ctggatctgt gtgaggaagt gcgtgcgcaa cgagcgcatg accgccttca
     5521 tgataggcag taactctgcg ctcttcctcg ctcataagcc ttgatttacg ctgcggccca
     5581 gccatcacgc gatcaatggc ttcatcgaca tcagttgatg aaattacctt gcgattttct
     5641 ctagcagtta aaagagctgc ttcatttaaa acatttgcta agtctgctcc ggtaaatcca
     5701 ggagtccttc tagcaatagc taagagatca aggtcttttt ctaaaggctt attcttagag
     5761 tgaactttaa ttattgcctc acggcccttt acatctgggc gatccacacc aatttggcga
     5821 tcaaatcttc caggacgaag tagcgctgga tctaaaatat ctggacggtt tgttgctgca
     5881 ataagaatta cctgttcatg agcttcaaag ccatccatct caacaagtaa ttgatttaaa
     5941 gtctgttcgc gttcatcatg accgccgccc attcctgcgc cgcgatgacg accaactgca
     6001 tcaatttcat caacaaagat aattgcaggg gcatttgctt ttgcttgagt aaatagatct
     6061 ctcacgcgag ctgcgccaac accgacaaac atttcaacaa agtcagaacc tgaaatggaa
     6121 tagaacggaa cctttgcctc tcctgctaca gctcttgcaa ggagagtctt tccggttcct
     6181 ggagggccaa acaaaagaac tcccttagga atctttgcgc caatttcttg atatttcttt
     6241 ggctctgcaa ggaaatcttt tatctctcta aattcctcta ttgcttcatc agctcctgca
     6301 acatctgcaa aagtaacttg cggcacttca ttgtcatgaa gctttgccct ggagcggcca
     6361 aaggaaaata ctcgtgatcc tccttgagat tggctcatga agaacaaaag taaaaatgct
     6421 agaagaagaa ttgggcctaa ggtaaagaga attgagacca gaatgttggt cttaggaacc
     6481 gttacatccc actctttcgc aggctgattt gcagtaagta aatcaataag aattggctct
     6541 tgccctgtga cataggaagc ctcaaccttg gtggccccgt ttattgccac cccaggtttg
     6601 agaattaatt gaatcttctg atctcggtca acgatgttgg ctgattccac atcgcctcta
     6661 gtaattgcag ccattgcctt tgaggtatca atcttggtga attgattgct gccagatgaa
     6721 atctgaccaa agatagttac gccaacaatt gcaactaaaa tccaaaataa cggtcgctta
     6781 aagaattttt gaggtgctgc cttcttgcca gatttagcag gaagttttga aggcttaact
     6841 tttactttct tatcggccat gtttactcat acatatgctt agcaagcacg gcaataaagg
     6901 gaaggtttct atacttttca ttgaaatcta gtccatagcc aataacaaag tcttttggaa
     6961 tatcaaagcc tacatatttg actgcaactt caacttgagc agaatctggt tttctaagaa
     7021 cagtcattat ctcaacgctc tttgctccac gggatttaag gttggcactc aaccaatcaa
     7081 gtgttaggcc agtatcaaca atatcttcaa cgattaatac atttcttcca gtaatgtctt
     7141 tatccaaatc tttcataatt cttacaacgc cagatgactt tgttccagag ccataggatg
     7201 aaacagccat ccaatccata tctaggtgtc tattgatata gcgcgataga tcagccatca
     7261 ccatcactgc gccttttaat acgccaatga gtaatagatc ttgattttta taatctgctt
     7321 caatctgggc agctatctct tttagacgga cttgtaattg ttcatcagtt acaactactc
     7381 gttcaatctc gcccttaact gattccagat ccactatcgt cctctcgctt tgtattaact
     7441 atccctgccc accgctttta gagagcgaaa gtctgcccga aattcgagcc acggtcacac
     7501 caccaggaag tgagataggg ccttgcccct tccaggctgt gatgagagcc tcaacaggct
     7561 caatgtgctc ggcagaaagt gagccctgcg gggctccagc cagataaata gctttcctta
     7621 ggactctagt tctaatggct cggggctgtg attcaagctt tgagatttct agcgatgctg
     7681 gctctaactt agaaaaaatc tcatcactta aataatccag cgcatcggca tcatcacgta
     7741 gcaacttagc tgatcttgca agagcatcac taatgcctgg gccaatctct ttctccattg
     7801 tcggaagcac tacttcgcga actctcactc ttgtaaattc cattgattga ttatgtggat
     7861 cttgccagaa cttaattcca gcttcctcac atgctgcctc tgtttgcgct ctagtgattg
     7921 aaagaagtgg tctgcgatag cgatcaactc ttgaggccat tccagatagc gagcgtgatc
     7981 cagatcctcg cgcaagacca agtagcactg tctctgcctg atcatctttt gtatgtccaa
     8041 gaaaaacagc aactgcgtta tgagagttag ctacatcatt taatgcttga tagcgcgctc
     8101 ttcttgctga tgcctcaagg ccatcagtga tttcaacagt gactcgtcta atctcaatat
     8161 tttcataacc aatttttgtc agcgttctct ttgtttcatg ggtgacatct gccgaattct
     8221 tttgcaagcc atgatcaata attaaagcaa ttactttaat tgactttgtt ttacactcta
     8281 gtaacaaagc tgcagcaaga gctgatgagt cagcacctcc agaggcagca actaaaacac
     8341 tatcgcctgc agcaatatct tctaattcag atctaacagc aagacggatc tgatagagcg
     8401 gggtggtttc ggccatgagg ctaagggtaa tcgccttaga cccggcctcc atatggcatt
     8461 aatcctttaa cgctatagat attggagaaa agcaggccct tatctttgga gtttgcctcc
     8521 cacatcattc cgtttccggc atagatactg atgtgatgaa tagtgtttac cgttcctttg
     8581 taggaataga aaagtaggtc acctggctca agctgactta gcggaacatg cttggtgtaa
     8641 ctgaaataga gagatgaatt gagacgatcc caatttggcc agccaagtcc agctgagcga
     8701 tatgcggcat aaactaaacc agagcaatca aaagcgtttg gaccttcatc gccccagaca
     8761 tatggttttc ttgctagaac ttgtttctta gcaaaagcaa cagcttctgc tcgcatttcc
     8821 ggagttgaac gggaagtgct gcgaccttta aagccaatgt caggccaaac cttggtgtaa
     8881 acattggtgg tagttgcaat atttgcattt gcttcttcta gtaatgcaag ttgtctctgt
     8941 tgctcaagag ttacgcgggt ctttcttgca gcattaagct cttttaatac ctgatcttga
     9001 atcttttgca gtttatcaac ttctttttgc tgggcatttc tagcagcatc agcttcaatt
     9061 ttagtttctt caactttttc agtagctacc tcttgtgctg ctttggcgta atctgcagct
     9121 ttcttggctt ttgcagcagc tgcttcagca gcttggaatc taaatagagc ttgggtgttt
     9181 tgatttccta aggtatccag cgttgagagg cgatctatca aatcttgtgg tccatcagag
     9241 tgaagaactg agtttaaatc agtaaatcca ccacccatga tgtaggcatt ggtggcaaga
     9301 cggccaatgg agtcatgggc tgcttttact tcactaaccg caactacata tgctgctgct
     9361 gctgattctg cctcttttgt tgcaattgct aactctcgtt tagctgcttc atatttagct
     9421 cttgctgcgt tggcaactgc agttaattgt tttagagtta aattcgcttt gcttaatttg
     9481 gcagccgcag catctgctaa tttctttttc tcagcttcag ctttctttgc tgcctcaatt
     9541 tgagcaagag ttggcttggg agtctttcca taggcagggc tatgggcaat tagaagtaat
     9601 aaagaggtgg aaagcgctat gaagcgggcg cgtttagatc tcaattcgtg gctccctggg
     9661 gattgggggt cagttgcttg agtgaaagga taaaggccaa ggctgggaaa tgcaggagga
     9721 aagaaggtgt gtcagttaaa aagtgatgaa tattgcagca accaagagaa tggaataaag
     9781 gcttaagtag gtaattgagc catgaaatat ctttgctgca gacttttcga tttgtgagct
     9841 tctaaggcta aacatctgta ctgcaaagaa aatagtgagg acagtatttg caaaataact
     9901 ccagtttggc aaacctgctg agtagagagt taggtatgaa gtagcaaaca ttgcaatggt
     9961 gtgaaaccac atctgctgtt caacttttgc aattggagca actactggaa gcatcggaat
    10021 acctgctgcc tcataatcat ctttatattt aatagctaat gcccagaagt gtggcggagt
    10081 ccagaagaag atcaccataa agaataggaa cgctgttgcg ctcagagaat tggcaactgc
    10141 tgcccatccg attaaaactg gcatgcatcc tgcaatcccg ccccagacta tgttttggga
    10201 ggtgcgccgc ttaagaccta cggtatagcc aaatacatag aagatgattg cgccgattgt
    10261 tagcgctgta gctagaggat tagtaaaaat ccagaagata gagagagata gaactgttag
    10321 cgctgttgca aaggcaaaag cttgattctc gcttaactct ttattaacaa tcggacgctt
    10381 tgatgttcta tccattaatt gatcgatatc agattcaata atcatattga aagcattagc
    10441 agcgccagcc gccagagttc cgccaagtag tgttgcaagg gaaattccaa gtggtggaac
    10501 gccatctgct gcaagaaaga gagcaggaag ggtggttact agtaataatt ccactacgcg
    10561 taacttcatt aacgcgatat aaggtgaaat catgtcttga gattaccctg tagattgcac
    10621 tggtgcgtat ctacttggcg tgggctatat ctctaaacct tatatttacc agcgcaattc
    10681 ctacctctgc agagccgcta tctgaaagct ttaagttaaa actagaagcc actattactg
    10741 gtgatatctc ccctaaatca gtgcgctcct ctaatactgg cttcatctca gctcataaca
    10801 tgatgtatcg ccatagcgtg acaatttatg atgctcaaag ccttgaatta gtgaaatcaa
    10861 tatcagatca ggtaaacctt aaagatctcg gtatcaaagg ctattccggg cttcatcgcg
    10921 gctccccagt tgaaggggca ttttcacctg atggtaaata tctttatgta acaaattatg
    10981 cgatgtatgg caagggattt aacagagaag gtactgataa atgtaatcct ggcaataact
    11041 atgaccgttc atttctctat cgcatcgata ctcagtcctg gtcaattgat gccgcttata
    11101 aagttggtgt agttcctaaa gttgtcgaag ttagcccaga taataaatac gttttagtaa
    11161 gcaactggtg ctcctatgac ctttctataa tttcaacaga gcttggcaaa gttgttaaaa
    11221 ctataaagat tggtgcatac ccaagaggaa tcaccatctc taatgactct caatatgcct
    11281 ttgttgccca gatggggggc tcagtagttc ataagataaa tcttgtaagt tttgaaagag
    11341 aactactaag tgttggctca aatccaaggg ctttagtcct ttctcctgac aattcctatc
    11401 tatatgccac tcttaatgct gcaggacgcg taattgcctt tgacctagct actaacaaag
    11461 ttgtggaatc agttaaaacg ggttcagcag ctcgaagcct ggatatctca gctgatggca
    11521 gcgcgctata tgttgttaac tacaccagcg acactcttgt gaaggtgcga agcagtgatt
    11581 ttaagatttt gcagaagatt aaagtttgca atgagccaat aggcgtaact tatgagagat
    11641 tacagaagcg agtatgggtt gcttgttatg gtggatcaat taaagttttt agtgattccc
    11701 catcacaata gcttccagca gtcgatccat ggcagccctt gctttatttc gtgaacttaa
    11761 cgttggtgtt atcccatcag ccataattgc aaagacatat tccttatctg ctgacttggc
    11821 atagccggca agagtcactg tatttctcaa ccagccagtc tttgctttca ctttacctac
    11881 tgcttcaggg ccatcttcaa taaatctctt ctttaatgtt ccgctcttgc ctgccacagg
    11941 aagtccttca tagatagctt gatacttagg gttgtctttt atttttacta gaagctcaac
    12001 tacggtttgt gttgacaggc gatttgcctt atctaaacca ctgccatcct ttgcttttag
    12061 accttcgcta ttaactccga tttcctccaa agctaattta aaggtttggg tgaggccttt
    12121 acttgttctc tcaaatccaa tctcgcgagc tgctgccatt gctaagcgat ttgccagagt
    12181 gttatcgcta taaaggttta caaacttgat catctcagaa agaggtggtg aagttaatga
    12241 ggcaatctcc tcttttgcta tctctttagc tttagctgca tcaactttct taaatgtggc
    12301 cttaatgcct tttctcttta gatttagatt taagtcatat cgatcttttt caaagaggct
    12361 tgagtagtaa atcgttaacc tctttttatt ctctgggttt gctttactaa taagtgaggg
    12421 gagatatttc tgcccgttct tcttagctaa agatagattg ctggtcatcc agggatctag
    12481 tgagcccttt attagatagg tattttcttt ctctgttgta aaaatagttg tgacatacct
    12541 tctctcccca cccacaaagt gaagtgctgc cgttgatgaa acaagtttta gaactgatgc
    12601 tggaacgcgc aaagattctg gagcgttctg cgcaattact tgaccatcta atggattaag
    12661 gatgagtagg cccggggtgg caagatgctt actatttaca tacttatcaa gagagtgaag
    12721 cgctgatgcc tcgagtgggt taatggctag caatagcgct agaagtagag caactcttga
    12781 tttagccatg gatgaaagag taatagtcat ttatccaact tggctttagg cttgagccta
    12841 tgcgtactgg ttatgtttat cacgagatat ttggatggca tgacacaggc acatttgtcg
    12901 gagatatgcc ttcagatcct ggcgcgggtc tgcaaccata tatgaattat gaaaatcctg
    12961 agactaagaa gagaatccat gaattaattg tggtttcagg gttgattgaa cacttaactc
    13021 gaattccagt ccgagctgca agcgagacag agttagcaac agttcacacc aaagaacata
    13081 ttgcgcggat aaagaaggaa tctgcttcaa gacttggtgg tgatggcggc gatttaacaa
    13141 cgccatttgc taaaggtgga tatgaaattg ctgctatggc cgcaggtggg gcaattgaat
    13201 tatttgaagc tgttctaaat ggcgatgttg ataatggata tgccctcatc aggccgccag
    13261 gtcatcatgc agtctctgaa actggaatgg gatattgcat cttttcaaac cttgcagtgg
    13321 caattgcagt ggcaaagaaa acacataaaa aggatcttcg agtagctgta gttgactggg
    13381 atgttcatca tggaaatggc actgaatcaa tatttttaaa tgatacaaat gtattaacaa
    13441 tctcgcttca tcaagaccag ttatatcccc atgacacagg aagtgttgat gttgtagggg
    13501 ttggaaccaa tataaatatt ccacttccac caggaactgg tcatggcggt tattactacg
    13561 cctttgatga agttgtaatt cctgcaatta agcaatttaa gccggatatc atcgcagttg
    13621 cctcaggttt tgattcctcg gtttatgacc cgctaggaag aatgttagtt actgctgaag
    13681 gctatcgagt aatgactaga aagcttatgg atgtggctaa tcaggtttgt gctggaaaat
    13741 taatgatgac ccatgaaggt ggttatagcg catcatatgc gccattttgt gggctctttg
    13801 ttttagaaga gctaagtggg gttaagaaat tagctgatcc atttgcccat ggaaataatt
    13861 atccaggtca tgagttaaaa gaacatgaaa agagaattgt tgatcaagca aagaagttgg
    13921 taagtaatct atgagtttgc cgccaggtga aattggcccc ggtgagtttt atcccgtagt
    13981 gggagtaggt cctcatccaa aaccatggcc aagtgatgag cactttgatc ctgaattact
    14041 tgaaaatggt gatcgccgca atgtccttga taagtatcgc tattggaaag ttgaagcaat
    14101 tgtcagtgat ctaaatacca agcgacatag ccttcgaata gcaatcgaaa attggcaaca
    14161 tgatttaaac attggctcag ttgtaagaac tgcaaatgct tttaatgtct ctagcgtcca
    14221 tatcgtagga aaacgcgact ggaatcgacg aggagcaatg gtcacagata agtatttaac
    14281 agtgattcac cacgcaacta tcgctgaatt caaagcttgg gctgatgatc ataaagtaga
    14341 gatcattggg attgataacc tagagatatc aaagccttta gagagcgcta aattacctag
    14401 agaatgtgtg ctatttctcg gtcaagaggg agctggtatg tcagatgagg cggtagccgt
    14461 ctgcagccaa gttctatcaa taaagcaata tggctcaact agatcaatga atgcctcagc
    14521 tgctggcgcg attgctatgt atgcctgggc tatgcagcac ttaaataagt aattgcttta
    14581 ttagtccatc tgttgctttt tctaccatct ctaaaacgtt ttcaaatgct gaatctggca
    14641 gtgagtatgg atctggaacc tctaatgaat caggtctact tgcatcaaat tgtcttaagt
    14701 aatagatctt gcgctcttct tcatcgcttg tgacatattt actcacctcg gcgtgattgt
    14761 ctttatccat tactaaaact agatcagctg ctacaagacg tgagtaatta aattgcgatg
    14821 caatatgttc aaaggaatat ccagcctttt cccaagttct cttcgagggg ggatttggtc
    14881 cttggccaac atgccaattt gaagtccctg cagaatcgac aaatatcttt ggacgctcaa
    14941 gctttgaaac tttattagat agaactgctg ctgccattgg agagcggcag atattgccgt
    15001 agcagaccat ggtgataatt aaattatcgc gccccttaag gtgtgagaga tctactgcgc
    15061 tcactaatct tcaattactt ctgagactga tttaattctt cgttcaattt caatcgcctc
    15121 atcggagcga cctagagtgt gaagaattgc tgcaattcgc cgctcaattg agactataaa
    15181 ttcaaagtct ttgagatcag cctcggctgc agtatcaagg actcgctcca aagtagtcag
    15241 accttcataa acctcgccag ataggacttg ggctttgccc agttcaaatg aggcataagt
    15301 taggcggcgt tgatcatgtg cggttgtaaa gacatcgaca gccttttggg ctgcagtgag
    15361 cgcagaatcg gcatcaccaa gttcggtata gcaatgagca attttctgat cacatcttgc
    15421 tacatgaatt acctctttag catctttgaa atatttcctt gcctcaaaga atgcctctag
    15481 cgctttttca tacatcttta actctcgata ggcacagcca attaaatgaa aatcagttgc
    15541 tgctcctagc tcatttccat caattaaatg ttcgcgggcg cactcttgca tgcaatcaat
    15601 ggtgttctga tactccttgg aactaaacca ccattcacca agcgtgcagg ccaattcaag
    15661 agccattggt gatttattct ctcgaagcaa ttccacagct ttactcatag cagttgctgc
    15721 ttcattcatt cgctttaatt gatttaggtt atagccaatg gctgaataag cctgcgctaa
    15781 tccttcacta ggagctgcgc ttccaagctc tgagtaaatg tcgcgagctg tttcagcaag
    15841 agctagagct tcatcgtatt ggccacgagc ataaattcgc gcgcttaact cgtaataagt
    15901 actggctcga tctacacctt gagtttcagg tattcgttcc cacaacattt cttcagtaat
    15961 gatttcatca ttaacatcat catcgttgtg ttcgtcggcc aatgcacttc tccattcact
    16021 agtagcgctg cgagtaagcc ttaacttatc aggtgttatt taagctggcc cttagaactc
    16081 acaattactg agagtcggcc ctgcccaaca ccactgagaa ggtctttgat ccaccgcctg
    16141 gcatatcaac tgtgatcttc acggtatcgc ctggggcaaa ggagcggact ctaacaattg
    16201 cagttattag atcgttgatc ttctttcctt cgatctctct aataatggcg cctgctggaa
    16261 tgccagcttt gtcagcggct tcccctggaa ctaatcgcaa tacctttgca cctgtgccgg
    16321 tgtagctagt atcaaaatta acgcctagga atggtctaga tgattttcca gtagcgatga
    16381 tttcatcaac aactcgcttt gcctgattaa taggaatcga aaaaccaagt ccaatagatc
    16441 cagaagttcc agaacttagc gttgcaatag cagagttgat tcctacaagc gctcctgttc
    16501 catcaactaa tggacctcct gaattgccag gattaatagc tgcatcggtt tgaattgcat
    16561 cgataaatga ttctgcgcca aagcttccag ttgtaacggg tctatttagc gctgaaacaa
    16621 ttccactagt tactgttcct gatagtccaa gtggggaacc aaatgccaca actggctctc
    16681 cgattgctaa cttactggag ttaccaactg aaattacttt taggtttccg cgatcaattt
    16741 ttagaactgc aatgtcgtag gtgctatctc ttcccacaat ggtagctttt tcatagtcgc
    16801 cattatttag ctcaactcta acgctgccac ttgttgctgc tgattcaatg acatgattat
    16861 ttgtaataat aaaagatgaa gaagcattgg tgcgataaat aacgccagat ccactaccgc
    16921 ctccagtagt tgcgctaact gcaatggata ccactgaagg tgaaacagca gaggcaatac
    16981 tttctacatt aactccagat ccggcaacct ccatcagggt gcgagttttg gtgcaacttc
    17041 cattagtcgc tgaataaagg gctttggttc gattatcgac acagaccttg gttgggggca
    17101 tatctggggt gaccgctaca gccacactgc cagctacaaa tgcgcccgca ataaagccaa
    17161 ccattacctt gctaaatgta gataaatttt tcatgcgcca aacctaacca ctattttctt
    17221 aatgaagtct taaagataat tcaaccttga ctgagtagac ttcgcaactt atggatcttg
    17281 ccctaagccg tgctcgcata gcggtaactg taactttcat cattaatggc tttagcgctg
    17341 gaagctttgt ggcaagaatc ccagacttta aaaggattct agatatttca aatgggaccc
    17401 ttggtctttc tttactcttt gtatcagctg gggtatttct agcccttaaa cctgccggta
    17461 aatactcagc aaaatttgga agtcaaccag ttattttctt ttcaacaatt gcactggctc
    17521 tctcttactt acttcttggt ttcctctttt ccctagcttg gttctggatc actttattta
    17581 tctttggatt tgtaatcgca acccaagatg tgtctatgaa tgctcatgct gtagtggttg
    17641 aacagcgtgc tgggcgacga ttaatgagcg tatttcatgc catgttctca gttggtacgt
    17701 tattcggtgg aatattaggc ggagtactct cgcaattaga gataactcca cttagccaag
    17761 cctcatcact tgcactcatt tatatcgttg ctgctcttct ggtgcgacca ctgcttcttc
    17821 cagcatctgc tgatactcac cactttggag atgagaagag agctaagcat ccgtttatct
    17881 ttgccatatt gggcctcttt ggtttgtttg cagctctaag tgagggagca gcaggtgatt
    17941 ggggcggagt cttagcccgt gaaacctttg gtgcatctcc cttcatttca actcttcctt
    18001 acattgtctt ctgcacggcg atgattattg gacgactctc tggtgattat cttgctcacc
    18061 gttttggcgc ttctaaagtc attgcagcag gtggggttat cgcaggaact ggtcttagcg
    18121 caggtttatt gatcggcggc attcctgcaa ttatggttgc ttggttttta cttggaattg
    18181 gtttatcggt agttattcct ttgatgttta gcgctgctgg aactattgct ttgactaaat
    18241 actctggagt tatcgctcca tctgaagcag ttgcaaaagt atctggcgtt tcctactttg
    18301 gttttgttat cggtcccccg cttattgggt ttattgctga tgcatttgag ctgcgctgga
    18361 ctttgatgtt attagcgggg ctttcttatc ttctgattct tgcttctaga tatgccaggg
    18421 tggcttaaga cttactccaa gaggtattaa gggctcgccc ctctgcatat cctgatgcac
    18481 tttgaattcc aatcacagct ttttgcgcaa attcacttaa attctttgct ccagcataag
    18541 tgcaagatga tctaagacca gcaataattt catcaatcaa atcttcaact ccaggacgaa
    18601 ttggatctaa atacatacga gaggttgaaa taccctcttc atataacgct tttctagccc
    18661 gctcaaatgc atcttcattt gaagttcttg ctgctactgc ccttgcagat gccattccga
    18721 aagactcttt atataaccga cctgaggaat caactcttaa atcacttgga gattcataag
    18781 ttccggcaaa ccatgaacca accatcacgg ctgatgcgcc agcagcaagt gctaaggcaa
    18841 catctcttgg atgtctgatg ccgccatctg cccagatatg tttcccaagt tttctagcag
    18901 cctctgcgca ctccatcact gccgaaaatt gtggtctgcc aactccggtc tgcatcctgg
    18961 ttgtgcacat cgcgccaggt ccaacgccaa cttttactat atctgcccct gcctcaatta
    19021 aatctttaac gccttctgca gtaacaacat tgccagcaac aattggaata gatggattta
    19081 atgagcgaac agcttttagg gcttcaatca tcttctcttg gtggccatgc gcagtatcca
    19141 gaactaataa atcaactcct gcttttatta attctgctgc ttttgtcttc acatctccat
    19201 taactccaag tgctgcgcca attcttaatc gcccctggtt atcaagtgct ggtttatata
    19261 gcgttgcccg tagcgcgcca attttggtga taattccaat taagttacca ctagcatcaa
    19321 ctactggagc aaggcggcga ttcttctctg ttaataaatc aaaggcctgc cgcgcgctaa
    19381 cagaacttgg aattaccatt aaatccttag acataattct gcttaattga gtgaagcgat
    19441 cagcgccctc ccaatcctct tccatcacaa ttcccagtgg cttattgttt tcaactacca
    19501 cgattgcgcc atgggctctc ttatgaatta gagatgctgc atcagcgacg gtctgctcgg
    19561 gcgttaaagt aattggggta tcaaagagaa tatctcggga tttcatccat gagatgactt
    19621 cagtaactac atcaattgga atatcttgcg ggataacact taaaccaccg cgccgtgaaa
    19681 cggtctctgc cattcttctg ccagaaattg cagtcatgtt ggcaactact aatggaatcg
    19741 tggccccggt attgtctaaa gtatttaaat caacatccat gcgactacta acgccagagc
    19801 gcgacggaat cataaagaca tcgtcgtagg tgagatcgtg atttggttga tgaattaacc
    19861 gcacgtagca gaactataga cctactagag cgcgatagga taaaacaatg tctgaagccc
    19921 ttatctctgc ccgtggctta actaagcgct acggggattt cacagccgta gatgccatcg
    19981 actttgatgt cgccaaaggt gaatcatttg gtctactggg tcccaatgga gctggtaagt
    20041 caacaacgat gcgaattatc gctgccacct cccagcgcac ctcaggaaca atcaccattt
    20101 tgggaagaga tcctgaagaa catggcccac aaatcagggc gcacctaggc gttgttcctc
    20161 agcaagacaa tctagatact gaactaaccg ttactgaaaa cttatttatt tatggacgct
    20221 actttggtct gagtaaaaag tttatccgca ccaaaataga agagctcttg gaatttgcgc
    20281 agttagaaga aaagcgcgat gtgaaagttg atgccttaag tggtggaatg aagcgtcgat
    20341 taacgattgc tagagcactt gttagtgaac ctgacatttt gatgcttgat gaaccaacta
    20401 ctggccttga tccccaggct cgacacattc tctgggatag gttgtttcgc ctcaaagagt
    20461 taggtgtgac tttagttatt actactcact tcatggatga agcagaacaa ctctgtgatc
    20521 gcctagttgt aatggataaa ggcaagatca tggctgaggg ctctccactc gagttaatca
    20581 agacttactc aacaaaagaa gttctagaag ttcgctttgg ttctgatagg aataaggaga
    20641 ttgctccaac gcttagggct atgtgctctc ggatggagga attgccagat cgaatcttga
    20701 tgtatgttga agatggcgaa gctctacttg aagaaatact aaacaaaaag cttcacccca
    20761 ctacctctct ggttcgccgc agctcactcg aagatgtttt ccttcgctta actggaagaa
    20821 ccttgatcga atgaattctg gtatcaacgt tgctaaagtg cgaagcatcg gagctatcta
    20881 tgtagcagag gcacgtatgt cagcgatgct taaatggaaa ggcatcattg caattgtcgc
    20941 aattggcaat ccactctttt atctaattgc aattggcatc ggagttggcg ttttagtaag
    21001 tgagaattcg ggaacttctg gaaccggcgg agttgaatat ataaccttta ttgcgccagc
    21061 acttcttgca agtgctgcaa ttactggagc tttggatgaa gcgatgtttc cagtaatgga
    21121 gggttttaag tggagaaaac tcttttatgc aatgaatgcc acacccatta ctgggccaca
    21181 aattgcactt ggtgttttta tcgccgcttt ggctcgagtt gcctttacag tgatttgcta
    21241 ttgggcagtg cttattgcat ttggagtaat gagtgttgaa caatcttggg atgtcatacc
    21301 ggtaacgata tttgctgcag gtgcatttgc agcgattatc atgggaatta ccgccaaagt
    21361 taaaaatgat gattacttca tgactgtcct tgggcgctta ataatcatgc ctatgtttct
    21421 cttctcaggc acatttttcc ctctcaacac aatgcctatt tacctacagc ctattggttg
    21481 gatctcaccg ttatggcatg cgacagaact tggtagatac ttttcatacg gatactcaat
    21541 tagcgctcaa atgttgatcg tccacttcgc atttcttcta gctctactta ttggtggttt
    21601 tgctcttgcc aatagaactt tcacaacgag gttgactaaa tgagtaacgc agttgcaatt
    21661 gcagatgcca gagccaaccg tgttggtcat cagctctatg ctgggcgcgc tcgagcaatc
    21721 attgaaagat ctttaattgc tcttcgctcc tcaacctggg ttccggtagc aacaggcttt
    21781 gtcgagccag tgctctatct gcttgctttt ggctatggaa tgggcgaatt aattggaaat
    21841 gtaactctgg gagataagac gattgactac acagtattta tcgcccctgg cttgcttgca
    21901 actagcgcca tgaatggagc gatttatgac tcaacgtgga atgttttctt taaattaaac
    21961 gagagcaagc tctatcaagg aatgctagca accccgcttg ggccacttga tgtagctctt
    22021 ggtgaaatca tctgggcgct atttcgaggt ttgagttatg ccattgcatt cctagctatt
    22081 accgcacctc ttggtttagt tcctggcgta tcagcagttt tagcaattcc agcagcagta
    22141 tttattgcct ttggctttgc ctcatttggt atggctatca cttcttactt taaaacctat
    22201 cagcagatgg gaatgataaa tatcgttctg ctgccactat ttctattctc gggttctctc
    22261 tatccaattt ctgtctatcc agattggttg gagtttttaa ttcaactaat gccttcctgg
    22321 cacggaatag aactagttcg tgatgtttgg tttggtaatt ttgattcagg aacattgcta
    22381 catatttctt attttctagt gatgattatt tgtggactct atttcacaac acgcagactt
    22441 cgtgatttat tcatgcggta aacacgcaca aaaatacgtt gtgttgacgg acctttaggg
    22501 ccgggggtag aattcgggaa agtttgatta acgaataggg gcgtggtacc aacaaatcgg
    22561 atcttttgat ccgtatttgg gaagtccaga ccaatcccat gttcaataaa actcatcacg
    22621 cggaaacttt tagatcaact ccttataaaa ctaaacccat ctctgctgct cgcataaatg
    22681 agctcttgga aatagagaaa gaaaagtttg caaaacttgc tccagaatct ggcgttgaaa
    22741 ataggcgcgc ctcgcaagta actccaaaag gcgtagcttc gagtttccag cactgggatc
    22801 catatccaat ttcaattgtt gcggctaaag gagcctggct aaccgatgtt gatggtaggc
    22861 agatgctcga tctctctatg ggttttggag ctcttctagt aggccactta aatgaagagg
    22921 tagtaaaaga ggctcatgcc accctagatg ttggcacctt atttgctact ccatctccta
    22981 ttacaagatc tggcgctgaa attatctgcc gcagatttgg tctagatatg gtccgctttg
    23041 ccaactctgg cactgaggcc acgatgtatg ccttaagaac agcaagagct gcgactggaa
    23101 aacttggagt aattaaaatt gaaggcggct atcacggttc atatgaccct ctaatggttt
    23161 cagcaaagcc agagatagac cgcgctggtg atgccaactc acctaatgca gttcctcttg
    23221 gagcaacagt tccaggcgat gtctgggttg ttccatataa cgatgctgag gcaattgaga
    23281 gagtattgaa gaaagatgca gatcaaattg cttgtgtatt tatggagcca gttctagaaa
    23341 atattggaat tgttcttcca gatcttggtt atttagaaag agttcgtgag ctctgtgatc
    23401 aatatggagt tttgctggta tttgatgaag taaaaactgg gctaactgca ggcccacaag
    23461 gtgctgctca gcgcctcgga gttaagcctg atctaatcgc aatcgctaaa tcgattggcg
    23521 gcggtattcc agttgctgcc tttggcggca agaaggaata tatggaagtt gtcgtcaatg
    23581 gaaaacataa tcatcttgga acatttaatg gtcattactt agcaatggca ggaataagag
    23641 cagttgatcg cctctgtact ccagaagccc ttaaaagatg tgaagattta aatagagatg
    23701 ctttaagtaa aatcgctgaa attattaatg agtatgagat tccagcccat acagttggct
    23761 ttggagtaaa gggctgtatt acctggtctg ccagcccagt tcgaaactat cgagattaca
    23821 aagctacaga ctttgatatt gcagagcttt ccttcttctg gtcactaaat aggaatgtca
    23881 tgaccccgcc aggacttgat gagcaatggc taatttctct tgctcatggt cagcgcgaga
    23941 tagacataat tgttggagat ttcttagagt ttgctaaagc acttcgcgct taagctcgat
    24001 agtagtgttc taagttccaa gccctaacac cacctgcaac tccagcagta tttggaacta
    24061 caaccacatc atcacccatt cgctctaaat ctattgggcg aatcaatctt gcatttccac
    24121 caccgagata taaccggtcc catttaaaaa ctggtcgaag ctcttctacc aaaactctaa
    24181 ttcttctcga ccagaatatt gtcccaagcc tcttgcgctc ctgctcgccg atccaagtgt
    24241 cataagtcgt tgctctgcgt actgtggcat gagagagttc aaagtgtggc gttaattttc
    24301 caccatagaa aattgcaaag ccaagacctg tccctaatgt aattacgact tcatatccag
    24361 agccggtaat aattcctgcg ccatgtacct ctgcatcgtt tagaactaac gttggaacat
    24421 ttagttttgt tgagattgct ttttgtgcat caaagccaga ccagagttca gcgctttctg
    24481 gatcaatttt tgtgcgcggc ccactctttg taatgtagtg cggggtagaa ataacaacgc
    24541 catttctaat cattcctggc attccaattg tggctctata ggccttagga agtcctgaag
    24601 caatctcggc aatagtctct acaaatgttg ctggcggaag tggataagga gttggaattc
    24661 tatttggctt ggaatgcatc gttcccgaac tatcaaggac gcagctcttg agatagaggc
    24721 ctccgcaatc aatcgagagc gttaggttct catccatacc cgcatcttcc cccacatgga
    24781 aatctttgcc aaatatttat caaaatcccc ttacaaaagt gctactacta gatagggtct
    24841 ttctatggct ttaattgaga ttcgcgattt ggcgaaatct tttggcaacg tcaaggcagt
    24901 tgatgacata tcactagata ttgagtcggg tgagttcatc accttgcttg gaccatctgg
    24961 atctggcaag accactgttc taagaatgat tgccggcttt gaggaccccg atgccggatc
    25021 aattaagtta aatggtgaag atataactca ccttcctccc tttgatcgcg atgtgaatac
    25081 tgtctttcaa gattatgcgc tctttcctca catgagcgtt caagaaaatg ttgagtatgg
    25141 attaagaacg agaaaagttc ccaaggctga aagagcgaaa caagcgctag aagctattgc
    25201 ttcggtgaag cttgaggaaa ctgttaaccg tttgccacat caactctctg gtgggcagag
    25261 acagagaatt gctctagctc gagctttagt tttaaggcct cgggtattac tactagatga
    25321 accacttggc gcacttgata gacagctaag ggaagaaatg caggttgaat taaaaaagat
    25381 tcaacgcgat gctggaatca cattcgtctt tgttactcat gatcaggaag aagctatgag
    25441 aatgagtgat cgaattgtgg tctttaactc tgggcgcata gagcaagttg gaactccgga
    25501 gcaggtatat gaggaaccaa gaactaattt tgttgctggg tttttaggaa ccgcaaatat
    25561 cttctccgta gatgtggctc gaaagttttt aggggccacc acaacagtaa gcattaggcc
    25621 ggagcggatt cgattacagg cgcctgaaac caagattgat aagagtgaaa cttgcgtaaa
    25681 ggcaactgtg caagaggcgg cctttgtcgg cgctaacacc atttacatac ttgagaccga
    25741 ttttggagtg aagttaacgg taagaaagac aaacacagaa ttgctggggc aagacaatag
    25801 ttttgtcgct ggcgatcagg taatggccgt atggcgaaat agccatgtag ctcagatacc
    25861 tgtttagtgc tgactcgagt cacacgaaag gaagaaaatg aaggggaaga aactagcaat
    25921 acttagcgct ctatccgcat ctgttttgat catcagtgga tgttcatcag gaagcgatga
    25981 gagctctggg gttgcaattg aagtgcccga tatcccaatg cacgaagcaa tgggcgaatt
    26041 tgagggcgaa ttaaatatcg ttaactggtc aggctttgtt gaaccagcct ggaccgataa
    26101 attcacagct gataccggat gcaaagtaaa tcctcgcgtt gcaggtacca gcgatgaaat
    26161 ggttaccttg atgcgaactg gtcaatatga catcgtttct gcatcaggtg atgccgctct
    26221 aagacttatt gtcggtggcg acgtacaacc attaaatctt gacctaattc ctaacttcag
    26281 cgatgacatt gccgctggta tgaagggaaa tatttacgac acagtaaatg gaaagcctta
    26341 cggtgttcct attggtcgtg gcgctaacct gcttcaatac aatgaagatg taacaggtgg
    26401 agcaccagag agttgggatg ttgtctggga gctaaacact ccttataagg gcaagatcac
    26461 tgcctacgac gcacctatct atattgctga tgctgcagtt tatttgatgt atcacaagcc
    26521 agagctaggt attaaaaatc cttacgctct agatgaaaca cagttagctg ctgctatcga
    26581 tctattaaag cagcagaatc aagttattgg cgagtactgg tcagatccag ttgctcagat
    26641 aacttcattc gtaggtggca acactgtcgt tggtacttca tgggaagttc ttcgtaagtt
    26701 cgccgctaaa gacaacctaa agacaacact tccagttgaa ggttcaactg gttggtttga
    26761 ttcctggttg gttagctcaa ctactaagaa catcaactgt gcctatgctt ggatggatta
    26821 caccagcaag ccagaggtaa atggcgctat cgctgttaac ttcggtatgg caccagcgaa
    26881 cgtcgcattc tgtgcttcca gcccagaagc tcaagctcac tgcgatacct tctttgcaga
    26941 agatgaagag tttttcaaga gaatttggcc ttggacaact ccaattgaaa cctgcgttga
    27001 tggtagaaaa gacgttaagt gcactagctt ccaagagtgg actaacgcct gggcaaccgt
    27061 taaaggttga taaatgttgt gtgggagtcg agtaatcgac tcccacacaa actaatcaaa
    27121 ataatatgtc aacaaaatca gactatcggg gcagtcgacc aatctcttcc ttctttctaa
    27181 gaaagccaaa ggttagattg atttctctct tgtctcttcc tatgacatgg attgttggcg
    27241 tttacatagt ctcacttttt cttctgctaa taacagcttt ttggaatgta gatcctttta
    27301 catcaagagt tagccctggt tttaatctag agaacttcat aatggttttt acagtccctg
    27361 cttatattgc aacatcaatt cgtactcttt ttatagctct atcggtaact gctatctgtg
    27421 tacttctagc tgtgccactt ggtatctaca tggcaaaaat tgctaagcct tggctcagaa
    27481 atattcttgc tgtagcaata actctgccac tttgggccgg ttacttagta aaaatcattg
    27541 ctatgcgact agttttcaca gaagaaggtt tctttaattg gttgatggcc ccactgggta
    27601 ttcgcgggcc tggattctca attccaatcg ttattttgac cctcacatat ctctggtttc
    27661 cttacatggc tcttccggta tacaccgcta tccgccagat tccaccaaat cttttcgacg
    27721 catcaagtga tatgggagct aaaggattta ctaccgttta ccgagttgtc attccactta
    27781 ttgttccagc cataattgca ggatctgttt ttacattttc tctgagttta ggtgattatc
    27841 tggctgctcg attcacaggt ggagcaacac aaatgatcgg aagcattatt gctgcaaata
    27901 tcaaccttaa tcccccagtt gctgctgcat attccatgat ccctattgct tttgtcgtga
    27961 tctatctaat ggtggcaagg cgcaccggat ctttggagcg catgtaaatg ttaaggctgt
    28021 cgagattaac ccgagtagcc cttggcggcg ttgtgctatt cattctattt tttatgtata
    28081 ccccgttaat ggtcttaata ctaaattctt tcaatgagtc ccagatttca ggctggccta
    28141 ttgaaagctt ttcaactaag tggtgggagt ttgctagaag ttatcaacca attagagatg
    28201 cacttcttaa ttcagttctt gtagcaactg gggctatggt tattgcctca atacttggaa
    28261 cgctagttgc ttttgctatg aaaggtttct ccttttttgg taaaggcact gtgaatattt
    28321 tgattgttct accgattgcg ctacctggag ttgtgacagg tgtcgccttt agcaatactt
    28381 actcgaattt ccttacgcca atgggattta atattggcta ctttggaatg attgtggcgc
    28441 actcaacgtt ttgtattgtg atggttttca ataatgtttt tgcacgtttg cgccggatga
    28501 accctaacct tcaagaagct tcagcagatc ttggagctgg cttatggcaa acatttaggc
    28561 tagtaacttt tcctcaattc agaacttctt ttgctgcagg agcgctttta gcctttgcct
    28621 taagtttcga tgaagtcgta gtaacgattt ttacagctcc gccaggagtc gacaccttgc
    28681 cattatggat tttgaaggaa gttggaaggc ctaatcaagc aagcgttgta aatgttgttg
    28741 ccactgttgt tatcttgctg tcgctcatac ctgtctatat ttcacagaga ttggctagag
    28801 cacaggaaga tgagcgttag agatctagtt ttttaggatt ctaagagccc gctttggtcg
    28861 atcagttatt actccataaa tctctctact tttaagccat tttagatcct cttttaaatt
    28921 gactgtccaa acatatattc tcttcgctct cattagattt atcaaaaatg ggtaacgttt
    28981 tagggtagtt attttgattg caacatattc atatcttgca aataatgcac tgatgccata
    29041 tcgaatgacc tttgcacact tggcatctct cttatctact cgtcgcacgg cccagaagga
    29101 aaatgacatg ataactacat ctattcctgc tgtatctatt ccttgcctat atttgtttat
    29161 caaatccatt gttctatatt ctatttcgcc actacttaac actggatgtt tagattcaat
    29221 caataaatcc tttttggctt caattgccat tactagcaat tcctctagcc ttattgcgtt
    29281 ggcatctctg attaattctt gagaactcac gcgcgacaca agtttgcgtc ttcccgtgat
    29341 tctcttggtg tctgaatcgt ggaaacacat aatttccttg tcttttgtta agcgaacatc
    29401 gcactcaaaa ccatcaacgc cagcttcgat agcagcccga taggcaattt gagtcatctc
    29461 gggatactta tgactaaatc cgcgatgggc aaaaactttc atcgcccgat tatgaaccta
    29521 agtgaattga gtattcccac tcgccctttt cgaaatttaa aaagttgaga tttgataagg
    29581 aatagcgccc gaaaaaatgg tgctattggg ccgaatactc gactaaagac cttggcgcgg
    29641 gcccctagat ccctctgagt tccccaagca cttccagaac tttcagatgg gtgtattgct
    29701 aatttaatag gtagaaaaac ccctttaagc ccttttttta tggaatctgc gataaaaatg
    29761 tactcatctc cgaggtaatt atctgctcct gcgccaaaat tctcgtcaaa ccttatcccc
    29821 gcatttctta tcgattcaat tcttatcata agttcataag ttgccgcctt ggcagaatta
    29881 aatctactca acttatgtgc tttttttgga taagctttac gaagtttgcc attctcatca
    29941 gatgtttgag ccataattat ggagcaatca gggtgagact caaagtaggc aatgcattcc
    30001 tgtaatcctt cttccacaaa agttatgtcg tcgtctccaa aaatcagata ctttcctgat
    30061 gcgtactgga ttgccgcatt tctactcttt gctacacccc tgctttttaa ttcaactaat
    30121 tttccttgta acttttcagt attgtaatta ctctcatcgg gattttgcac taggacaatg
    30181 atttcttgat cagggctact tttggggtat ttaatatttc ctaagcgctt agttaatgtt
    30241 gagtaagcaa ttgttaagat tacttcattc aaggtcatcg tctgaatcaa ttcgcgcact
    30301 tgactttgca taacgcatta atgaacgtga agtattagag attacagaat tctgtagtcg
    30361 cgctccgaga aaaacgatta gagcatttgt tgggcttatc tgcgaatcta cgcttaaata
    30421 caacagtgca gcaagcagga ctagcgttcc tgcgctggag acaagcgccc ctgactctcc
    30481 cgcctttatt cttgcgctgt agcgtttctc aggctttatt cgtttctttt gattccttat
    30541 cgcaagatct ttttgaacca tgacctgctt attaaatatc cagcctaaag cctgcagaac
    30601 gacaattaat atcaaagtgt ttattagtgc cagaaggggg tctaggaagt aaaagaaggt
    30661 gattattgct actaattggg tggcgaaact aagaatgttg ctgagctcaa aggccatcgc
    30721 ccactctcta ttttcttcct tcacagtgcg gtctttttta gctgctctga aggatctggc
    30781 aaaagccttt actcgatagc ttctctggaa atattgactt acccgggtca gaataaagaa
    30841 gagaaaaaat cctgcaattg cccatcctag aaactctcga gtgaattctt cctcgttcaa
    30901 aactaaagtt gcaaagaatt tcataacaag aagttcagag acagatattg tcgcggcaag
    30961 cgccgatagg gcgattaaca cgattcgaaa tttgcctcta gggaatagat agcgaaaccc
    31021 actgatcatc tctcttgcaa tagcaatcat ttggtttctc gcattccatc aagctttctt
    31081 gatcctaaga tagaaattag gtgaccaata gttacaaaga gggcaacaaa tatccctatt
    31141 tcaatcaatg caggattatt cccaaagatg gctataacga tagacatttc aaccgagtga
    31201 aataggcgtt gtacggcaaa gattttgaat ggtaatttaa taattcttag ccagttcttt
    31261 gtaggggcac cactttcttt actatctcct agttttgaaa gtccagcgaa agctcttgat
    31321 acatgaacgg catcattaaa tcctttattt aataagattg ctatggagat taaagctgca
    31381 ataaaaggca acctccagtc agaaatttca ttttcgggcc aacctgaaag tcgaagcgca
    31441 aaggcaatag gaatcaagct ttcagttaga tagtgggcga ctctgtccaa gaaaatgcct
    31501 ttgggggatt gagtattttt ccatctagca atttccccat cacaacaatc ccagagcatc
    31561 tgtagctgag agagaaaaag agctagcaaa ataccttgat agccagggat tagtaatgca
    31621 gcacttatag atagtcctgt tgcaatcatc aaatatgtca ctccgttagc tgttattgga
    31681 gtttttagta gaagccttgt taagtaaggt gagactttgc gcaaatagag atcagcaacc
    31741 caatgctcag cattggctct tccagtgact gaggctggct gggaaatctc tcgaatctgc
    31801 gcaatcgtcg gattcttcgg cctatctatc attatccaac cttattggct ctatgactta
    31861 aaacccattg caaagaagaa attactaaga atattgttga aaaataccag gcaaagtaat
    31921 tcaaactcca gccactgaat gcagctattg ctagaactaa taatcgcccc tcgataaata
    31981 acccaaggct tgagagccag attggtttct tctcattttg aagcgagcga taaaggttgt
    32041 catagtggtg aaatacgatg gcaaagaaaa taacaaatga gaccatattt atctgctcat
    32101 ttcttatgaa aatccataac aaaaaggcaa actcaattac tctcaagaaa gagggttcta
    32161 tccagtcgaa tctcttcaga aatgagagtc ttacaaagag agaatcttgc tgcgcgctta
    32221 tcaatattcc tcgacttcgc tccttttgca tcttaagagt gcggatcgtt cgacctcgaa
    32281 aaacgactaa ggcagatata aaagctaata tcggcattac tgtgaatgtg aaacttgccc
    32341 cgccaataac agcgctagcg ctgattacca accagcgctc accgatcggg aattgaataa
    32401 tcttgcctag ccaataacgc aggcggcctt taggttcacg ttctgaagga atgattccat
    32461 cgaaatcggc cgagaaatcg attggagcta agaattgctc ttcaactcgc tgctttattg
    32521 agcgagcaaa gttgtagtct gaaagatgcc taaaggtttg tatcaacatc atcactagtg
    32581 ctgggatcca taaatcctgc cctgatttct cagcacctat agcaagtcct agaaacacca
    32641 tgtattcctt gactcgatct gtcacagcat caagccaagc gcctaatttg gaaaattttc
    32701 tggtgtagcg cgctagctct ccatcaacac agtccacaat gatgcttaac tgtagtaata
    32761 ctgctcctag taaagtttga gaaaaagttc ctttcgtgaa acaaaatgct gaatagagac
    32821 caattgcaaa agatattaaa gtcacctgat tcggtgttgc atttactctt accgctaacc
    32881 aagttagaag cttagaaaac ttacgtaaga aaaatacgga gtaaaaccca tcatttgctc
    32941 gatttgccat cttaagtctt aatttgccca tgtttaaatt agcaagctcg ctcttaactt
    33001 tctctcttac taattcatct gagctacgcg cgtaaggtgc tgcccaaagt tcagcagcat
    33061 cgacttgcac tctagctctc gttaatgcaa ccaagaccag atctatcgca ttaccagata
    33121 gttcagaatt ggccgcacct tctaatgcct ttattacctc atctcgttgt ctctgagaga
    33181 ttcgaatagc acctgcaaag ttacgattac caattgtgac tttatgtgaa cttgatgaag
    33241 cgctagcaat ccgattttga cgaaccaatg tgtcgccatt tttttgggtt gagacaagag
    33301 ctactgaggc gcttcttgga taatcagtga ggagttctaa atgtggttgc gcaataactg
    33361 agttcagatc caaaagtagt aagtcgctat ttgaagcttt aaaactatca aggatttctc
    33421 tgacctttgt tgaagaggta acaacgagag aagtaaggga tttatccatt tcctgtagta
    33481 taaatctatg acccttcaag ttgtgattct tgcagctgga atgggcaccc gtcttggaaa
    33541 accgtggcca aagccgctta cccccttagc tgatggtcgc tcgattatgg cccaacaatt
    33601 agaaaatgtt gagaaagtct ttgggcaaga agctcgagta acaatcgtag ttggattcaa
    33661 gatggaaatg attatggagg ctcatccaaa tgcagccttt atctataacg aagtttatga
    33721 tcaaacaaat acatcaaaga gtcttctaaa agcgctccgt gcatcccaag agagtggcgt
    33781 tctctggtta aacggagatg tagtttttga ttccagagtt ctcgagcgcg tgcaatcacg
    33841 catgcgtgag gagaaatcct ttgtctgcgt gaatacttca gcaacatcag atgaggaaat
    33901 taaatacact gtagataaag atggaaatat taattctttg tccaaacaag ttaaaaatgc
    33961 cttaggtgaa gcggtaggaa tcaacttcat tggcagcggc gataaagcgc atgtaattac
    34021 tcacctagag gaatgtgctg ataatgacta ttttgaacga ggccttgaat tagccataga
    34081 gaaagcaggt ataaagctcg agcccgttga tatatctgat ttgtttgctg ttgaagttga
    34141 tttccaagca gatttagaga gagcaaataa aggactggtc taaatctgat tacgagtacg
    34201 agaaatttgt actcgcaact ttcttcgata acgtttgact aaactctttt taatccaact
    34261 taacggtaac ttcttgatta gatctttatc aaaagccaac atcgcttggg caatatgtgc
    34321 tatcgctatc tctgttggat aaaccgggtc tccctcgggc accgatgccg aatctaaagt
    34381 attgatatct ccaatgattg ttgctccgct tgctacgagc tcatctttgt tctgcgcgcc
    34441 aatctcatta cctttatcta ttgcccattt aggggttagc aacttagctg aatcacttgg
    34501 agacttaatt tcatcagtta atcgccttat atatccatcc ctgataaaga tttcatactc
    34561 attccaggtt ctatctttag gaaattgtct atttaattct ataagtaggg ctatttctgc
    34621 aagatttaaa gaacggttac tacctatttt ttgaggcttg ataaagtctt tgggtagccc
    34681 taaatattga ttaattgagt cataaagaaa ttctggtttt gattcgtcca caattagtac
    34741 agttacgttt cctgttccca ggacttttgc ccactttgta accacctgcc catggaaatg
    34801 acgcatccaa aaagttggat tgatctttga ctcaccgggt ttatctagta ccgaatgcag
    34861 ccaggtcaca tagtctgccg taatcccata ttttaaatat tgctgataag aagagctaag
    34921 aagctttacc aaaggcctaa cggtaaagag cgcttcaatt tttcttcctt taatctcatt
    34981 aaagattgtt tgtatctgtt ctgtatccag ctctgagaag aattcgctac tgagtaatac
    35041 ggtttcagat ttatctgcat tgatggcctt tactagacga gtccattctt tctttgaagt
    35101 tttctcaccg ccttttttat tccagcccca cattctttga gataacgccc aagcaatccg
    35161 atgatgggct tttcttccga tatttggata gacaattcca ttttgcttta actcaaggct
    35221 ttgagagtca aaggattcct gcagagcagt agttcctgat ttgtggaatc ctgcatgaat
    35281 tactaaacga ggttcgctca ttaggcaatt attctatgag tctttacttc gatagtgagc
    35341 taagggataa gttttcatca taaagtgctg agaattgctg ctctaacgcc ttgaacgaga
    35401 tctttcaatg cccaactagg ttgatttgga tattgggcta tctgctcatt tactaatgga
    35461 aggtgaacaa agccagattt aactctgctt tcaagaagtt gatgttggag atggtaaaaa
    35521 atgtgattac agagaaatgc tccagcagat agtgagattt cactttctag cccgcactct
    35581 ttgaccgctc caaccatttt ctcgatcggc agagtgctga aatacccatc tgcgcccttt
    35641 tcaataatta tcttatttac tctcaattcc cctgcattgt cgggaattcg agctgaatca
    35701 agatttattg caatcttctc tggagttaac gctcttctgc cttctgcttg gccgaaggca
    35761 atgattatct ctggatttat ctctttaact ttagaaagta gaacttctgc tgccttatca
    35821 aactcaacag gaagaattac tgtaaaaagc tccagtcctg taaagctctc tttagaaatt
    35881 tcctccacta caagctgtga ggaattcaag tcagatccac caaatggctc aaagccactt
    35941 attagtactt ttttgcctgt agacaaatta gctctctaaa ttagttaaac accgagaaat
    36001 gctgcgccaa ccaaaagtgt tgcccaaagg atatgaagtg gcagcatcca tttagcaata
    36061 gtttcaattg ctgctttatc aacttcatct ttgcttgaat cttttctcaa ttctagtaac
    36121 tcgcgaagcc ctaccgacaa aactaatagt gtcgctaccc atgcaaccat gcaataaggg
    36181 caaagtgcgg caatctcaaa aatggtttgg aaagctaacc aatggcagaa aattgcagcc
    36241 aaaacagtcc ccgaaactag aagctgccaa aagagctttg gaaatctaac gccaagaaat
    36301 aaagctacag aaatcacgac catcatggca aaagtagcta ttccaattaa tgaatttggg
    36361 aaaccaaata cttctgcttg atctgaaagc atcacgctct tacagttgat agttgcattt
    36421 atattgcagg agggtgtgaa gccagaatcc tttaggactt tgaatttatc tatcgtcaaa
    36481 ataaaggaag caaaaaaccc aattatccca gcaaaaagta gggtccaagc tgaactaagt
    36541 tgcttgatca tcacttggca agaatacctc tttggttgat tctttgatac tcgatggtaa
    36601 ccccacaagc gttgtattct tacgacctat gtataacgat gaagattgga aagatgacct
    36661 tcgagagaag aagcgtctaa gtgatgaaga aattcatagg aataaaagat cttggaagaa
    36721 actttctttt attgcgctat taataattgc agttatcgga gtctttaaag gttaattatc
    36781 tggccaatta gaagttaagt catagagaag tagaagggct gccgagagga tacctccaac
    36841 ccaaattcca actccaacgc cccattgcaa ctcaatggaa gcccaaacgg ttgcgatgaa
    36901 gatagtaacc atcgctagat caaagattgc tctctctttt gaatccatgg cataagcata
    36961 gaatagagtg atttaaatac gaagtaggtc gataccttgc tatagattga gcgccttagg
    37021 tggcgtaatc ctgaaaggtt acttaaaaat aatgcgtttt atgataaatg taattgaaag
    37081 tattgaaaaa cctgcgcatt caaggcagga aattgatgcg attaatgcct tcaatgatga
    37141 gattgtcgcc gcaggacagc gggtattagc tgttgggatt gcttctccag ataattcaat
    37201 tctgattgat aatcgagatg gagatattaa gtacattaat gagagtttta atggctccag
    37261 cgagtatgta agtggcattt ggattattga tgtccctagt agagaaattg ccttagagct
    37321 agcagctcgg ggttcaaagg cctgtaatcg cagagttgag ctaagaccat tactaggtta
    37381 gtagatctgc ttagaaaact tctgcaagtt cttcttggga taactcttat ctataccgga
    37441 acgctacatc taacaacgca aagattagag ttccaagctc aagttccacc ttggttaggt
    37501 cttgatccag attttgtagt acttgcctcg ggctatgttg agatagcact tggtatttca
    37561 ttgcttactt tgtggaaatt tcgccgagaa gttggattgg ccacagcgat cttttttatt
    37621 ctcattttcc ctggaaatat ccgtcagtat gttgatggca tcgacgcctt tggtttagat
    37681 accgatcgaa agcgagcgat aaggctgtta tttcaaccgc tcttagttct atgggcgctc
    37741 tggtcaactg atgccttaaa taaatatatc ttgcgcgccc gtagggattc gaaccccaaa
    37801 ccttctgatc cgtagtcaga tgc
//
LOCUS       DAOROD010000010        37187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_31_length_37187_cov_33.355569, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000010 DAOROD010000000
VERSION     DAOROD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 37187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37187
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_37187_cov_33.355569"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..110)
                     /locus_tag="PLW53_03390"
     tRNA            complement(37..110)
                     /locus_tag="PLW53_03390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Gly,seq:tcc)
     gene            219..294
                     /locus_tag="PLW53_03395"
     tRNA            219..294
                     /locus_tag="PLW53_03395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:252..254,aa:Pro,seq:tgg)
     gene            327..1613
                     /gene="tig"
                     /locus_tag="PLW53_03400"
     CDS             327..1613
                     /gene="tig"
                     /locus_tag="PLW53_03400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRD36500.1"
                     /translation="MKSAVETISKTRVRILIDVDFTDLEPHIKTAYQSISERIVIPGF
                     RKGKVPRAMIDQRVGRGAVLDEAINNGLPEFYSQAARENDLLVLGRPSVDIKELKDNE
                     AVKFEVEVDIRPEVSLPDFSKIEISVDDVAVSDDDVKEQIEALRIRFGTLTTVEKDVE
                     KGDFVTIDLSAKVDGAEIEGGSANGISYEVGSNRMVDGLDEALIGMKLEQVKKFNTQL
                     VGMDEGQSGEVEVTLKAVKKRELPELNDEFAKLASEFETLAELDADVRQRLTRLKAME
                     QGAQARDNLLKLLLETVEIAVPENLVKEEVDAHLEKENRLEDSEHRSEVSLEITNSLK
                     ADFLLDTIVKSEAVQVSEAELTEYLIRSAARYQMSPDQFAQQISQAGQIHALMAEVAR
                     SKALAVVLERVGVKDASGRKVDLAALAPKQESGSEQ"
     gene            1742..2335
                     /locus_tag="PLW53_03405"
     CDS             1742..2335
                     /locus_tag="PLW53_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRD36501.1"
                     /translation="MASSQGGGLDDQVYNRLLKERIIFLGSVVEDSMANAIAAQMLLL
                     AAEDPDKDIYLYINSPGGSISAGMAIYDTMQYIKNDVATVGMGLAASMGQFLLCAGAP
                     GKRYALPHARIMMHQPSGGIGGTASDIKIQAEQMSFTKKKMAELIAFHTGQKMETITA
                     DSDRDRWFNAEEAKEYGFVDHVVKSAGQVSGSGGTAR"
     gene            2332..2955
                     /locus_tag="PLW53_03410"
     CDS             2332..2955
                     /locus_tag="PLW53_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRD36502.1"
                     /translation="MKINDIQGRYVLPTIEERTAYGYKRLDPYTKLFEERIIFLGQGI
                     DDTVANDVMAQLLTLESMDPDRDIMMYINSPGGSFTALTAIYDTMQFVRPDVMTICLG
                     QAASAAAVLLAGGTAGKRYALEHSRILIHQPYSEGGGQGSDIEIQAREVLRMRELLEE
                     ILAKHSKKSKEDIGKDIERDKILTAAEAVEYGLVDQILASRKASVTK"
     gene            3050..4336
                     /gene="clpX"
                     /locus_tag="PLW53_03415"
     CDS             3050..4336
                     /gene="clpX"
                     /locus_tag="PLW53_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRD36503.1"
                     /translation="MTRIGESGDLLKCSFCGKTQKQVKKLIAGPGVYICDECIDLCNE
                     IIEDELSEASSAGIQELPKPQEIYEFLDQYVIGQDRAKKSLSVAVYNHYKRIKGPDRR
                     IEDGVELSKSNILLLGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGEDVENI
                     LLKLIQAADFDVKKAETGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTT
                     ASVPPQGGRKHPHQEFIQIDTSNVLFIVGGAFAGLEKIIENRKGKAGVGFNATLQSEK
                     DKKAQDLFADVMPEDLLKFGMIPEFIGRLPIVTSVEALDRAALLKILTEPKNALVKQY
                     QHLFEMDDVEFEITEAALEVVADQAIARGTGARGLRAIMEEILLPVMYEVPSRKEVGK
                     VLITPEVISDKAAPKYLERGPNNPKRSTKRGEEKTA"
     gene            4359..6947
                     /locus_tag="PLW53_03420"
     CDS             4359..6947
                     /locus_tag="PLW53_03420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRD36504.1"
                     /translation="MSELPAQFNPSQIESELYQKWLSAGYFTANADSGKEPFSIVIPP
                     PNVTGSLHIGHALDHSIQDLLSRMKRMKGFEVLWLPGMDHAGIATQNVVEKQLAATGV
                     SRHDLGREEFVKRVWKWKEESGGAILGQMKRLGDSVDWSRERFTMDQGLSDAVLTIFK
                     QLFDAGLIYRAEKIINWCPRCLTALSDIEVEHSDDAGEFVQVRYGDDEVSITVATTRP
                     ETMLGDGAVAVNPKDERYKHLIGKSVLLPLVNRMIPIIADELVEPDFGTGAVKVTAAH
                     DPNDFEMGMRHNVPLIVIMNEAGVMEGTGTEFDGMDRFDARVAVVEKLRQMGRVVSEK
                     RPYIHAVGHCSRCETTVEPRLSKQWFVKVAPLAKAAADAVRDGRVKIDPESMEPRYFE
                     WVDNMHDWCISRQLWWGHRIPVWYGPDGDVIVVGPNQSAPKGYEQDPDVLDTWFSSAL
                     WPFSTLGWPNKTDDLKKFYPTSVLVTGYDILFFWVVRMMLFGLFAMDGVVPFKVIALH
                     GLVRDKHGKKMSKSRGNTVDPLEFMDKYGSDALRFTLVRGANPGTDQALAEEWIAGSR
                     NFATKVWNAARFAMINGAHVNGELPNRSELNHIDRWILNRLDSTIASADKGYEAFEFA
                     KASEEIYHFAWDDLCDWYLELVKESFAKGGEQADKSRRVLGHVLDQLLRLMHPSMPFI
                     TETIWCELTGGESLVIAKWPAVSKAAPDVAADKVVGDMQVLITEIRRFRSEQGIKTSA
                     RINAQISGLEGDLLNYEDAVRFLVRLDKPASDFKESARIEIGQFSIAFDLRGAIDVKA
                     ERARLSKDLENIKKDLQSAVVKLENENFMAKAPMEVVREIRERMEFCESEITRINTLL
                     AALPKE"
     gene            6950..8296
                     /locus_tag="PLW53_03425"
     CDS             6950..8296
                     /locus_tag="PLW53_03425"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRD36505.1"
                     /translation="MSSAEDLSRLDAIEKALLNRWPETRIAPTLERISTLADYLGSPQ
                     LSYPTIHIAGTNGKTTTTRLIDSLCFELGMRTGRFTSPHLESFLERITINGQSITPEF
                     MIATYNDIAPYLELVDSKMTNKLSFFESMCALAFVAFAEFPVDVGIFECGMGGEWDST
                     NVINGSVNVITPIGFDHMEYLGDTLEKIAQTKSGIIKQNSFVVLAEQESDVAAVLMRA
                     CAKADATPIRSGIEFSLKSRALALGGQMLTISGVYGEYEDLYLPLYGQHQASNAATAL
                     AAVEVFAGETKLDEEIVRQAFANATSPGRCEIVGRNPTIIIDAAHNPHGAESLKKTIS
                     EEFDFSALIGVIAPMGDKDVTGILEALEPVLDRVIVTRNSSHRAADVDELKSLAMEIF
                     GSDRVSACDDLEQGITQAIDMARTSNALNDSNTAVLIAGSVVSAGEARAIIRRKGI"
     gene            8302..8646
                     /locus_tag="PLW53_03430"
     CDS             8302..8646
                     /locus_tag="PLW53_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HRD36506.1"
                     /translation="MKMLARSVLIMEFLIIGFALLVAKDLSTKNSLYFGALIALLAFF
                     SSGLLKYKVGWALGWLTQILLIAYGFFIPTMFILGSLFLGLWVAAIILGRKGEAAKAA
                     HIAKAEGPDSGS"
     gene            8668..9105
                     /gene="ndk"
                     /locus_tag="PLW53_03435"
     CDS             8668..9105
                     /gene="ndk"
                     /locus_tag="PLW53_03435"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRD36507.1"
                     /translation="MSNASTPAERTLILVKPDGVRRGLVGEVISRIESKGYKIVALRM
                     LTADKDLLATHYAEHQGKPFYEPLLEFMSSGPIVAMIAEGQRVIEGFRSLAGATDPTL
                     ASPGSIRGDLARDQGTKVVQNIVHGSDSVESAQREIKIFFPNL"
     gene            9117..10157
                     /locus_tag="PLW53_03440"
     CDS             9117..10157
                     /locus_tag="PLW53_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRD36508.1"
                     /translation="MAKMKTDWSFLGRDMAIDLGTANTLVYVRGRGIVLNEPSVVAVN
                     QDTGAVLAVGTEAKKMIGRTPGNIVAIRPLKDGVIADFDTTALMIKYFIRQVHKRTYL
                     AKPRIVICVPSGITGVEQRAVKDAGYEAGARKVYIIEEPMAAAIGAGLPIHEPTGNMV
                     VDIGGGTTEVAVISLGGIVSALSIRVGGDEIDHAIINWVKKEFSLLLGERTAEEIKMA
                     IGSAYPLRDEPDAEIRGRDLATGLPKTIVVSSEDIRKAIEEPVNAIVNAVKGTLDKTP
                     PELAADLMDRGIVLTGGGALLKGLDERLRHETNMPIHIADRPLNAVVEGSGKCVEEFE
                     ALEKVLISEPRR"
     gene            10165..11082
                     /gene="mreC"
                     /locus_tag="PLW53_03445"
     CDS             10165..11082
                     /gene="mreC"
                     /locus_tag="PLW53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRD36509.1"
                     /translation="MATRGSNRSRLLWISLLVTSLFIITLDLRGVSLLSSLRSGTQSV
                     LAPVERLANNFFSPVGNFFSEVSNLGRSKAKIEKLEEENQELKSQVIFNENTLAQLES
                     LKGVLDLAGKARYKTVSARVISKGGTGTFSETIVLDIGSDSGVRRDMTVIAAGGLVGV
                     VKSTTASSSIVLLMNDPSFRIGVRVAGKQDMGILLGQGDKSYSLQMLSATSAISVGDV
                     LLARGSSGDKPFVPGVPVGRISYVENTVGQLTKEGRVNGFVDLNSLSVVSVVLSAVAG
                     DPGDALVPQAPRPAPTVTVFVTPSPTPKE"
     gene            11087..11605
                     /gene="mreD"
                     /locus_tag="PLW53_03450"
     CDS             11087..11605
                     /gene="mreD"
                     /locus_tag="PLW53_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRD36510.1"
                     /translation="MEINRLPKVLLIFLSLFLLQEALINQIDFLVAGFSLYLAFFMAW
                     IIQDQRNSAVVTGFIAGLILDLSPTLEAPFGLWTLVLTSMSYLLASNVRAALDAELSP
                     LVMLSVAVVASSFALILFLLFGAILGQEVGSASYLIRSISGNALWSALLSPLYIPLAM
                     KLYKVTLTARQR"
     gene            11606..13672
                     /gene="mrdA"
                     /locus_tag="PLW53_03455"
     CDS             11606..13672
                     /gene="mrdA"
                     /locus_tag="PLW53_03455"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD36511.1"
                     /translation="MRDRSALTLLVAQVLVISLMLSLFGRLFYLQIADGPRYQEAALD
                     IQSRDIVTPALRGLIVDHEGNALATNKAGLMVTADRSVLDKLEDKGVSVLTRVAEVLK
                     LEYSDIYTRTRLCGELASGERNGCWNGNRYQPIPITKDASENQVFTILEKSDLFPGID
                     AKPVSIRSYPSLAGQKATHVLGYLGPITEKNLNNEEGKRYYRNELIGKAGIELLYDEQ
                     LRGVPGIRTVIVDRKEIVRQESLNRAPVPGNHLVLNLNAKLQAAVEVELKDAVFRARA
                     NGYRGDSGAAIVMDVKTGEVLAMASYPDYDLNIWENGITVNQARDLYSEKSGVPALSR
                     AIQGEFAPASTFKVVSLSAAVDAGYSLKTTYECPAEVQIGDRTFKNFDSKAAGRISMT
                     TAMAISCDSIWYQIAYDEWVRDGGLKPKPSPNDYFFDSARGFGVGKKTGVDLPSELSG
                     RLPDRQWKLNWYEKNKDFYCNYTEKAKKSQLTPYLIEIARENCLDGDKVRAGDMVNFS
                     IGQGDTLMTPLQLAVAYSALANGGKLMVPKVAKAIVEPSGRVVKEFKPEISGRLPIKK
                     STLKFLQNSLRAVVTSGTATGAFSGVGVEVSGKTGTGQVFGRNLDGSSKDDTSWFASF
                     APSKKPEYAVVMMVSQGGFGASTSGVGVRKIYEHIFGTNGRVAIFPNGTPKKLPRIDS
                     AKVALP"
     gene            13669..14811
                     /gene="rodA"
                     /locus_tag="PLW53_03460"
     CDS             13669..14811
                     /gene="rodA"
                     /locus_tag="PLW53_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRD36512.1"
                     /translation="MKISLKGQGKKGFFAGTDQVLNLAVFMLLLIGIVLVYAATRDWF
                     AANGLDPEYYLKRQIVNVIIGIALAYGTTLVDYRLLRAYTPFLWGFGVLGLIAVLIPG
                     LGSTVNGARSWISFPGGLQFQPAELAKISIIVGMALILSERMAGEDRPSDRQVLQALG
                     VAALPIILIVAQPDLGTVLIISAAVITMIALSGASLYWVIGLLIAGVVGVYIAIASGE
                     VSEYQLNRLKSFVDPSADPQASGYQLRQARITIGSGGLLGKGLFDGPQTNGRFVPEQQ
                     TDFIFTVAGEELGFLGSSFILLLYFIVLMRAFAIARATTDLFGKMVCIGVVAWFSFQI
                     FENIGMTMGLMPMTGVPLLFLSYGGSAMFANLIAIGLLQNVYQRTR"
     gene            15017..17116
                     /locus_tag="PLW53_03465"
     CDS             15017..17116
                     /locus_tag="PLW53_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRD36513.1"
                     /translation="MAEEPKKVRKRAVKKSASKAVEQSESAPAAKEKKSKAGVIPAPI
                     FQAAPVAAAPKAKPATTKPAAKSSAEVSGESEAERENRRRRRRRGGRSRRRLEKDESP
                     TTSSELSQGPSSDSEGSVGFKKRRRRRRSGAEGVAPGEKIEEDGVLTVVKVREPRPVQ
                     EKPVREKKSFDRPPRRFKDRDRDRGRDKPRYNDYRENRRRGTILTDAEFLARRESVDR
                     EMLVRQQGDRTQIAILEDKVLVEHYVNRNSNISYVGNVYLGKVQNVLPSMEAAFVDIG
                     KGRNAVLYAGEVNWDAAGLADNQERKIEKVLKTGQSVLVQVTKDPIGQKGARLTSQIS
                     LPGRYLVYVPGGGMSGISRRLADTERSRLKEILKGLITDEEGVIVRTAAEGASDEELT
                     RDVIRLKAQWDDIKAKSENTSTSAPSLLYGEPDLTVRVIRDIFNEDFRKLTVQGSDAW
                     DDINNYLGHIAPELLTKIEKYSQGNDLFAENRIDEQLAKALDRKVYLPSGGSLVIDRT
                     EAMVVIDVNTGKFIGKGGNLEETVTKNNLEAAEEIARQLRLRDLGGIIVIDFIDMILE
                     SNREQVLRRLVECLGRDRTKHQVAEVTSLGLVQMTRKRVGQGLIEAFSTTCDSCGGRG
                     IHVHMEPVNKKSLDREFVPAKVNKEENLSSDDSESDEKASLDENLTPAKKDGDSGQKK
                     GFGRRKRRAASSGVVTA"
     gene            17212..17526
                     /gene="rplU"
                     /locus_tag="PLW53_03470"
     CDS             17212..17526
                     /gene="rplU"
                     /locus_tag="PLW53_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRD36514.1"
                     /translation="MYAIVKAGGRQEKVAVGDTLFVDRIDAAAGSTVSFPAVLVVDGS
                     AVTSDPKLLSGVKVTGEVISEVKGPKIHILRFKNKTGYRRRQGFRSKNTRVKITAING
                     VK"
     gene            17541..17795
                     /gene="rpmA"
                     /locus_tag="PLW53_03475"
     CDS             17541..17795
                     /gene="rpmA"
                     /locus_tag="PLW53_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRD36515.1"
                     /translation="MASKKGVSSTRNGRDSNAQFLGIKRFGGQVVNSGEILVRQRGTK
                     FHPGKNVGIGKDDTLFALASGSVEFGSARGRRVVNIVPVS"
     gene            17881..19359
                     /gene="obgE"
                     /locus_tag="PLW53_03480"
     CDS             17881..19359
                     /gene="obgE"
                     /locus_tag="PLW53_03480"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRD36516.1"
                     /translation="MTTFVDRVTLHAAAGKGGDGCVSVHREKFVPLGGPDGGSGGRGG
                     DIILVVDPSVTTLLDFHHSPHRSASSGRQGYGALKNGPDGEDLILGVPNGTVVMNKEG
                     RILADLVGNGTRFIAAKGGLGGLGNNALASNRRRAPGFALLGEPGEKRTLILELKSVA
                     DIGLIGYPSAGKSSLVAAISAARPKIADYPFTTLAPNLGVVQAGEARFTVADVPGLIP
                     GASQGKGLGLQFLRHVERCAALVHVLDCGTLETERDPVRDFEVIEDELKKYAEDLSTR
                     PRIIALNKVDLPDAKAMADMVEPTLKKMGYEVFQISAASRIGLEQLLYAMAKLVAEHR
                     KQESAQEKVKIVLRPTPIDDVGFKTMANDDGSFTVIGSRPERWVHQTNFSNAEAVGYL
                     ADRLASLGVEKELFKLGAKQGDEVRIGLGDDAVIFDWEPTIEAGAELLSGPRGDDMRI
                     PRGWEKFDEEAIDQLDDEELAQQWEYKVEDPTDPGVEMEEQK"
     gene            19356..20468
                     /gene="proB"
                     /locus_tag="PLW53_03485"
     CDS             19356..20468
                     /gene="proB"
                     /locus_tag="PLW53_03485"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRD36517.1"
                     /translation="MNRDAVIKAKRIVIKIGSSTLTGADGAGLDSAAVTKLAAAVAEL
                     KEQGREVVVVSSGAIAAGLAPLGLGKRPADLATQQAAASVGQGLLIHSYTEALRKHKI
                     IASQVLLTIEDIVRRSHYQNAQRTLFKLLALGVVPIINENDSVGTQEIRFGDNDRIAA
                     LVSHLIGADLLVLVSDVDALYDANPKEAGAKKISQVDSLAELADAQIGGAGSKVGSGG
                     MVTKIEAARISTQAGIPMLLTCLADVSKVLAGSELGTYFVASTDKKSSRLLWLAHAAS
                     SSGRLIIDAGAVSALRERGTSLLPAGVKAVEGEFSSGDTVEITAEDGSAIARGLVAFD
                     SSEIPAMLGRSTKDLAKELGPEYERELVHRDDMVLL"
     gene            20465..21733
                     /locus_tag="PLW53_03490"
     CDS             20465..21733
                     /locus_tag="PLW53_03490"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRD36518.1"
                     /translation="MSNDQLVADLADKARKASKTLITAGYSKRQGALLAIADALEANM
                     GEILEANKLDMERGKASGLNSSLLDRLALSPERIKDIASGARQVAALDDPLGIVLRQR
                     TLSSGLKLTQITVPFGVIGMVYEARPNVTVDAAVILLMSGNAALLRGSSSAKASNEVL
                     VKVMRGALEKGDISSEVIQLIPSSDRDTTRALLHARGKVDLVIPRGSAELIRMVVDDS
                     TVPTIETGAGVCHVYIDKSADFSKALPIVINSKTHRPSVCNAAETLLVHQEIAKDFLP
                     LALKELHQAGVILHCDQISLEVANSLSIPASLATEENWSTEYGVLEMNVAVVPDLDSA
                     AAHILKYGTNHTEAIVTESNESAERFIALADCAAVMVNASTRFTDGQEMGFGAEIGIS
                     NQKLHARGPMGLEQMTTTTWIVRGDGQIRK"
     gene            21772..22068
                     /gene="gatC"
                     /locus_tag="PLW53_03495"
     CDS             21772..22068
                     /gene="gatC"
                     /locus_tag="PLW53_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRD36519.1"
                     /translation="MSNISRDDVANLAKLARIDLSEDELAGLAKELSVILDAVARVQE
                     VAGEDVPPTSHPLPLVNVFREDVVRPSLTPQEALSGAPEQEEQRFKVPQILGEE"
     gene            22068..23552
                     /gene="gatA"
                     /locus_tag="PLW53_03500"
     CDS             22068..23552
                     /gene="gatA"
                     /locus_tag="PLW53_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRD36520.1"
                     /translation="MINKSAVEMAEALNKGEITSVELTKAHLKQIGEVDGQVHAFLHV
                     DTQGALDQASQVDAKRSAGEKLPALAGVPLALKDVLVQKGIPTTCGSKMLEGWRPPYD
                     STVVRKLKENGIVILGKTNMDEFAMGSSTENSAYGPSRNPWDLTRIPGGSGGGSSASL
                     ASFQAPLAIGTDTGGSIRQPAAVTGTVGVKPTYGGVSRYGLVAFSSSLDQAGPCARTV
                     LDAAYLHEAIAGHDPLDSTSINAPVPAVVAAAKDGNIKGMKIGVIKELQGDGYQAGVL
                     KLFNESLEILASLGAEIVEVSCKYFDYALAAYYLIAPSECSSNLARFDAMRYGIRNGQ
                     GSAEEVMNATRQIGFGREVKRRIILGTYALSSGYYDAYYGSAQKVRTLISRDFESAFK
                     LADVLVSPTAPTTAFKIGEKSNDPIAMYLNDIATIPTNLAGIGGMSLPSGLAAEDGLP
                     AGFQIMAPAMKDDLMYKVGAALEAELNTKWQGPILNSAPKLGAK"
     gene            23552..25054
                     /gene="gatB"
                     /locus_tag="PLW53_03505"
     CDS             23552..25054
                     /gene="gatB"
                     /locus_tag="PLW53_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRD36521.1"
                     /translation="MALKYEDVIAKYEPALGLEVHVELGTASKMFCGCNTVFGAAANT
                     QTCPVCLGLPGALPVVNGKAIEYTIAIGLALNCSIAPYSRFARKNYFYPDMPKNFQTS
                     QYDEPICTNGYLDITIDTDEGSKDFRIEIERVHMEEDTGKSLHMGGATGRIHGADYSL
                     LDYNRAGIPLVEIVTKVVNGCGKYAPEVARAYVSELRDILRGLGVSDVKMEQGSLRCD
                     ANVSLRPIGSEKLGTRSETKNVNSLKSVERAIRSEMIRHAELLNSNQRVIQETRHFHE
                     DTGLTTSGRSKEQAEDYRYFPEPDLVPIAPSKELIAKIAATLPEKPSVRRKKLQEAWQ
                     VPDKEMAAMINADVLDLVEATVKLGADPTRARSWWLGEIARIANEKAVAAADLAISAA
                     DVAEVIALIVKGELTDKLARQVVEALINGEGSVAKIIETRGIKVVSDDSSLMAAIERA
                     IAAAPDVAEKVRGGNIPASGALIGAVMKESKGQADAAKVRDLLLKHLGQG"
     gene            25068..26744
                     /gene="ilvD"
                     /locus_tag="PLW53_03510"
     CDS             25068..26744
                     /gene="ilvD"
                     /locus_tag="PLW53_03510"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD36522.1"
                     /translation="MTNPMKPRSGLVTDGLERAPARGMLRAVGMKDEDWVKPQIGIAS
                     SWNEITPCNLSLDRLAKASKQGVLDAGGFPMQFGTISVSDGISMGHEGMHFSLVSREV
                     IADSVETVMQAERFDGMVTFAGCDKSLPGMMMAAARLDVASVFVYAGSTLPGQVDGKD
                     VTIIDAFEAVGACARGLISQEQVDKIERAICPGEGACGGMYTANTMATVGEAIGLSLP
                     GSAAPPAVDRRRDSYAVKSGAAVVELIRAGITTRDILSKQAFENAITALMALGGSTNA
                     VLHLLAIAHEAKVDLTLNDFHRIGSKVPLLGDLKPFGRFVMSDVDKVGGIPVILKALL
                     DAGLLHGDCLTVTGKTMAENLADINPPDPDGEILRAVKSPMGVSGGITILSGSLAKDG
                     AVTKVAGVGVDSFEGPARVFDREQAAMQALEDGTITAGDVVVIRYEGPKGGPGMREML
                     MITGAIKGAGLGKSVLLITDGRFSGGTTGLCVGHVAPEASDGGAIALVKDGDRIRIDI
                     NARTLDLLVDESELAKRRESFKPLPSRYTSGVLGKYVKLVGSASKGAVCD"
     gene            26977..28698
                     /locus_tag="PLW53_03515"
                     /pseudo
     CDS             26977..28698
                     /locus_tag="PLW53_03515"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            28719..29279
                     /gene="ilvN"
                     /locus_tag="PLW53_03520"
     CDS             28719..29279
                     /gene="ilvN"
                     /locus_tag="PLW53_03520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRD36523.1"
                     /translation="MAQHTLSVLVENSPGVLARVASLFSRRGYNIDSLAVGPTESPEI
                     SRITIVLNLEGHALDQVSAQLFKLVNVIGIQEMEDKNSHQRELLMVKVNCTGDNRSKI
                     EAVVGKYRASIVSQASSSLTIEAIGSSAEIAALLGELSSFGIKELVQSGLIALENGDA
                     TLAERTLQETGLASSSTHSQTEDYQN"
     gene            29298..30332
                     /gene="ilvC"
                     /locus_tag="PLW53_03525"
     CDS             29298..30332
                     /gene="ilvC"
                     /locus_tag="PLW53_03525"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRD36524.1"
                     /translation="MATIYHDEDADLSVIQGRKVAVIGYGSQGHAHALNLRDSGVDVR
                     VGLAEGSKSRAKAEEEGLRVLSVAEAVKEATVIMLLAPDHKQRHIYKESIEPNLKAGD
                     ALFFGHGFNIRFGYIKPPANIDVCMVAPKGPGHLVRREFAAGRGVPDIVAVEQDATGS
                     AWPLTLSYAKAMGGLRAGGILTTFTEETETDLFGEQSVLCGGASQLVQYGFEVLIEAG
                     YQPEVAYFECLHELKLIVDLMYEGGIAKQRWSVSDTAEYGDYISGPRVIDPRVKENMK
                     AVLGDIQSGAFAKRFIEDQDAGAPEFKSLRAKGESHPIEEVGRNLRKMMSWVKAANKD
                     DYKEGSASRG"
     gene            30325..31917
                     /gene="serA"
                     /locus_tag="PLW53_03530"
     CDS             30325..31917
                     /gene="serA"
                     /locus_tag="PLW53_03530"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRD36525.1"
                     /translation="MAKPKVLIAEELSPATLDALGPEFEIINCDGANRSELLACLSSG
                     VDAVLIRSATKMDSEAISAAKGLKVIARAGVGLDNVDIPAATAAGVMVVNAPTSNIVS
                     AAELAIALLLASARHLSPAHAALKGGKWARSKYTGAELFEKTLGVVGFGRIGQLVAHR
                     MQAFGMKVIAYDPYLQPAKAAALGVDLVSLDDLLASSDFITIHLPKTKETANLIGDAA
                     LSKVKPTVRIINAARGGVLDEAALYKAISQGRVAGAGLDVFATEPCTDSPLFTLDQVV
                     ATPHLGASTDEAQERAGIAVAVSVRKALSGELVPDAVNVKGGEIHEEIRPTLPLVEKM
                     AQIATGLEAELPVSIEITVKGEVSIYDSSILATSALKGALIAIGTEDATYVNSPNLAQ
                     EKGMSATVTSEAECEDYRSMICLRAAFSNGSRVEVDATLMGIRKVEKIIRINKFDIEL
                     PPSDHLLFLVYEDKPGVVGAVGNILGAAKINIANMQVARDKAGGEALMALTVDSAIPI
                     EFTEKIAKEVGARVSRSVSLLV"
     gene            31914..32954
                     /locus_tag="PLW53_03535"
     CDS             31914..32954
                     /locus_tag="PLW53_03535"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRD36526.1"
                     /translation="MSKNFNLAVIAGDGIGPEVVNEGLKILDAAGKKHGFTYSKKAFE
                     LGAAFWHKTGEVLPDKTLEELKSFDVILLGAVGDPSVPSGVLERGLLLKLRFAFDHYI
                     NLRPARLYPGVVGPLKTSAPIDFIVVREGTEGPYVGAGGVLGFGTSSEIATEESLNTR
                     RGAERVIRDAFNRAMARPRKKLTLVHKNNVLTRAGSLWTRTFEEVAKEFPQVSTDYLH
                     VDAASMFFVTNPERFDVVVTDNLFGDILTDIAAAICGGIGLAASGNINPSGEFPSMFE
                     PVHGSAPDIAGKGIADPTATIMSVAMLLNHLGLSDAARDVEKAVEADLLTRADKKRST
                     SEIGDDLAKALS"
     gene            32954..34039
                     /locus_tag="PLW53_03540"
     CDS             32954..34039
                     /locus_tag="PLW53_03540"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRD36527.1"
                     /translation="MKITVIPSSNPISPTQRDQYLEAPGFGKYFTDHMAVAEWNQSSG
                     WSDLTISAYGPLNLDPAAMVFHYGQEIFEGMKAYYQPDGSIALFRPDANALRFAKSAA
                     RITLPELPVEDFVSAVEALVKQDAGWVPKKVGESLYLRPFMIATEVGLGVRPSNKALF
                     AVIATPAAAYFNSAKAVTVWISLEYVRAVLGGTGEAKCGGNYAASLLAQQEAAKEGCD
                     QVVWLDAKERFWVEEMGGMNLYFVKGRGKDATIFTPKLTGTLLHGITRDSILSVAKDL
                     GYKVEEGMISIDQWRDGVASGEISEIFACGTAAVIAPVGSAKSKYGTWVTGDGNPGPI
                     TMEIRNHLLGIQHGTIPDKHGWMKRVI"
     gene            34039..35628
                     /gene="cimA"
                     /locus_tag="PLW53_03545"
     CDS             34039..35628
                     /gene="cimA"
                     /locus_tag="PLW53_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRD36528.1"
                     /translation="MAISDSFHIYDTTLRDGAQQEGLNLSVHDKLTIARHLDDLGVGF
                     IEGGWPGANPKDTEFFKLAAKELKLKNATFVAFGATRRANTKAADDVLLRALAESGAP
                     VVTLVAKSHDRHVELALKTDLQENLAMIKDSIKFLRQGGQRVFLDAEHFFDGYRANPA
                     YALEVVRVAAEAGADVIALCDTNGGMLPDELSDVVAKVISDSAARIGIHCHNDTGCAI
                     ANSLAAVAAGASHVQGTLNGYGERTGNADLISIIANLQLKKKQEVLPAGALEEAFRIA
                     HAVAEVTNVSPSARQPYVGISAFAHKAGLHASAIKVDPALYQHENPESVGNDMRMLVS
                     EMAGRASIEIKSSQLGFDLSKEKEVVARLVERVKELESGGYTFEAADASFELLLREEL
                     AGKKPSFFTIESWKTSVDQGADGSVTSRAEVSIKAKGELISSSGSGNGPVNAIDRALR
                     NGLEKLYPELAELELTDYKVRILEGRLGTGAITRVLVESSDGHGEWSTIGVHENVIAA
                     SAMALEDALTYGLIRAGKKPE"
     gene            35662..35925
                     /locus_tag="PLW53_03550"
     CDS             35662..35925
                     /locus_tag="PLW53_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD36529.1"
                     /translation="MRTVLKRGVKLTPSESSEWLRARMEQLRISGLEELHLKTGIDKG
                     SISRYFRQERTPKIDVIAPLAQALEVSPETLLIALGAIDKKRS"
     gene            complement(35926..36162)
                     /locus_tag="PLW53_03555"
     CDS             complement(35926..36162)
                     /locus_tag="PLW53_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36530.1"
                     /translation="MKILLVISDTALEPSLTNTATEIRVTIGINDDFDQILDVTSGIL
                     DTEQIAHLHRLWADDAFARDFNRTGDELIITARE"
     gene            36328..>37187
                     /locus_tag="PLW53_03560"
     CDS             36328..>37187
                     /locus_tag="PLW53_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRD36531.1"
                     /translation="MALDSELAKAYRSHLISERNLSENSIRAYLADLESLLLHINKLG
                     VSEFAQLELNHIRSWLANLSTKGAARSSITRRVVSIRAFTYWGARSGWLSRDIGKDLI
                     APKPERSLPDVLDIESAALTIKALEVRAQEEESASSLRDLALVEVLYGSGIRISELVG
                     LDLGDIDRQRSTIKVMGKGSKERVVPLGQPALTAVDNWINKGRAELVSEASGSALFIG
                     SRGKRIDQRVARSVVYQAMEAIGSDKKLGPHTLRHSAATHLLEGGADLRTVQEILGHS
                     SLATTQIYTHV"
BASE COUNT        10577 a         7246 c         9250 g        10114 t
ORIGIN      
        1 ctagttggaa gcgtcactgg atgatttaga aaagattgga gcgggcgacg gtaatcgaaa
       61 ccgcgtggct agcttggaag gctagggctc taccattgag ctacgcccgc actttttatc
      121 tttctaaggg ggtgctaatg ctcctcctta ggggcgcaag ggtagcgctt ggggtaatta
      181 cttgtgaatt tttcatctag actctgcttc ctgcgccgcg gggcgtagcg tagtggctag
      241 cgcgcctgct ttgggagcag gagaccgtgg gttcgaatcc ctccgccccg accagatata
      301 cctcttcata ttaagtcagg agccatgtga aaagcgctgt tgagacaatc tcaaagactc
      361 gagttcgtat tttaattgat gtcgatttca cagatcttga gccacatatt aagacggcat
      421 atcaaagcat ttcagagcgc attgttatcc caggctttcg caaaggaaaa gttccaagag
      481 cgatgattga tcaaagagtt ggccgcggag cagtccttga tgaagctatt aataacggcc
      541 tgccagagtt ttatagccag gctgctagag aaaatgatct tctagttctt ggccggccaa
      601 gtgttgatat caaagagctt aaagataatg aggcagttaa atttgaagtt gaggtagata
      661 ttaggcctga agttagcctg cctgattttt caaagatcga aattagcgtt gatgatgtag
      721 ccgttagtga tgatgatgta aaagagcaga ttgaagcttt gagaattcgc tttggaacgc
      781 tcacaactgt tgaaaaagat gttgagaagg gcgactttgt cactattgat ttaagtgcga
      841 aagttgatgg cgcagagatt gaaggcggaa gtgctaatgg gatctcctat gaagttggct
      901 caaatcgcat ggttgatggc cttgatgaag ctttaattgg aatgaagcta gagcaggtaa
      961 agaaatttaa tactcaatta gttggaatgg atgaaggtca aagcggcgag gttgaggtca
     1021 cgttaaaggc cgttaaaaag cgtgaactac ctgagctaaa tgatgagttt gcaaagcttg
     1081 caagtgaatt tgaaacactt gccgaattag atgccgatgt aaggcaacgc ctaactcgcc
     1141 taaaggcgat ggagcagggg gctcaagcaa gagataatct cttgaaattg ctactagaaa
     1201 cagttgaaat tgcagttcct gaaaatctag taaaagaaga agtagatgct catctggaaa
     1261 aagaaaatcg cttagaagat agcgagcata gaagtgaggt aagccttgag atcactaatt
     1321 ctcttaaggc tgacttcttg ctggacacaa tagttaagag cgaggccgtt caagtctctg
     1381 aagcagagct caccgaatac ttaattagaa gcgctgctag atatcagatg agccctgatc
     1441 aatttgcgca acagatttct caagctggcc agattcatgc tctgatggct gaggttgcaa
     1501 ggagcaaagc cctagctgta gttctggagc gagttggggt taaagatgcc tcaggtcgaa
     1561 aagttgattt agctgccctt gctccaaagc aagagagtgg atctgagcag tagtttctct
     1621 gctctaagcg aacaaaccac ctttttcgat attgcaggaa gcatttttct attcttggcg
     1681 ttagcctaac ggacatagaa atgtatatca ggaggaatta gtggagctca taaccccccg
     1741 tatggccagt agtcaaggcg gcggattaga tgatcaggtc tataaccgat tactaaaaga
     1801 aagaattatc tttcttggtt ctgtggttga agacagtatg gccaatgcaa ttgctgccca
     1861 gatgttatta cttgcagcag aagatcctga taaagatatc tacctttata ttaattcacc
     1921 aggcggttca atctctgctg gtatggcgat ctatgacact atgcaataca tcaaaaacga
     1981 tgtagcaaca gttggcatgg gccttgcagc ttctatgggc caattcctac tatgtgctgg
     2041 agcacctgga aagcgctacg cgctgccaca tgctcgcatc atgatgcacc aaccatcagg
     2101 cggtattggt ggaacagcat ctgatatcaa gattcaagca gagcagatgt catttactaa
     2161 gaagaagatg gcagagttaa ttgcatttca cactggtcag aagatggaga caatcaccgc
     2221 agattctgat cgtgatcgtt ggtttaacgc cgaagaggct aaggaatatg gctttgttga
     2281 tcatgttgtt aaatctgcag gccaagtatc aggaagcggt ggtacagcgc gatgaaaata
     2341 aacgatattc aaggccgtta tgttcttcca acaattgagg agagaactgc ttatggttat
     2401 aagcgtcttg atccttacac caagttattt gaagagagaa ttatctttct aggccaggga
     2461 atcgatgaca ctgtggccaa tgatgttatg gctcagttat tgactcttga atcgatggat
     2521 cctgatagag acatcatgat gtatatcaat tctcctggtg gctcatttac cgccctaact
     2581 gcaatctatg acacgatgca atttgttaga ccagatgtca tgacaatttg tcttggacaa
     2641 gctgcctctg ctgctgcagt tttacttgct ggcggaacgg ccggaaagcg ttatgcgctt
     2701 gagcattcaa ggattttgat tcaccagcca tatagcgagg gcggtggtca aggctctgat
     2761 attgaaattc aagcaagaga agtcttgcgg atgcgcgaat tacttgagga gattcttgct
     2821 aagcactcca agaagagtaa agaagatatt ggtaaagata ttgagcgcga caagattctt
     2881 accgcagcag aagctgttga gtatggtctt gttgatcaaa ttcttgcctc aagaaaagcc
     2941 tctgttacta agtaggggta attactctca gggcgaacgc gtttagccat ctcttgctgg
     3001 gctagaggat ttattcttat cttcttcccc tctagaaagt aggctcatct tgactcgcat
     3061 cggtgaaagc ggcgatctgc ttaagtgttc attctgtggc aagactcaga agcaagttaa
     3121 gaaattaatt gctggtcctg gtgtctatat ctgcgatgaa tgtatcgatc tctgtaatga
     3181 gattatcgag gatgagttaa gcgaagcaag ctctgctggg attcaagagc ttccaaagcc
     3241 acaagagata tatgagtttt tagatcagta tgtaattggc caagatagag caaagaagtc
     3301 gctctcagtt gcggtctata accactacaa gagaataaaa ggaccagatc gccgtataga
     3361 agatggcgtg gaattatcaa agagcaatat cttgcttctt ggtccaaccg gatgtggcaa
     3421 gactctgtta gctcaaactc ttgctcgcat gctaaatgtt ccttttgcaa tagcagatgc
     3481 cacagcgcta acagaggcag gctatgtcgg tgaagatgtt gaaaatattt tgcttaagct
     3541 aattcaagca gctgactttg atgttaagaa ggcagagact ggaattatct atattgatga
     3601 aatcgataag gtggcaagaa agagtgagaa tccatcaatt acaagagatg tttcaggtga
     3661 gggagttcag caagcacttc tgaaaattct tgagggaact accgcctctg ttccaccgca
     3721 gggcggaaga aagcatcctc atcaagaatt tattcaaatc gacactagca atgtgctatt
     3781 tattgttggt ggcgcttttg cagggcttga aaaaattatt gaaaaccgca aaggaaaagc
     3841 tggcgttggc tttaatgcca cgttgcagag cgagaaagat aagaaagccc aagatttatt
     3901 tgcagatgtt atgccagagg atcttcttaa atttggcatg attccagagt tcattggccg
     3961 ccttccaatt gtgacctctg ttgaagcctt agatcgggcc gcacttctta agattcttac
     4021 cgagccaaag aatgctctag ttaagcaata tcagcatctc tttgagatgg atgatgtgga
     4081 gtttgaaatt actgaagcgg cacttgaagt tgtggcagat caggcaattg ccagaggcac
     4141 tggcgctaga gggcttagag ccatcatgga agagattttg cttcctgtga tgtatgaggt
     4201 gccaagcaga aaagaagttg gcaaagttct aatcacccct gaggtaataa gtgataaagc
     4261 agcgccaaaa tatctcgagc gcggtccaaa taacccaaag cgttcgacta aacgaggcga
     4321 agagaagacc gcgtaataat cggtagtatc tacatcttat gtctgagcta cctgcgcaat
     4381 ttaatccaag ccagattgaa tctgagctat atcaaaagtg gcttagcgct ggatatttca
     4441 cagcgaatgc agattcaggt aaagaacctt tctcaatagt tattccgccg ccaaatgtca
     4501 ctggcagtct tcatattggc catgcgctag atcactcgat acaagattta ttaagtcgaa
     4561 tgaagcgaat gaaaggcttt gaagttttat ggctgcctgg catggatcat gcagggattg
     4621 ccacccaaaa tgttgttgag aaacaattag cagcaacagg agttagtaga catgatttag
     4681 gaagagaaga gtttgtaaaa agagtttgga agtggaaaga ggaatcaggc ggagcaatct
     4741 tgggccagat gaaacgccta ggagatagcg ttgattggtc gcgcgagaga ttcacaatgg
     4801 atcagggcct ctctgatgca gtattaacga tctttaagca gctctttgat gcaggattga
     4861 tttatcgcgc agagaagatt attaattggt gcccgcgttg cttgactgcg ctctctgata
     4921 ttgaagttga acatagcgat gatgctggag agtttgtgca ggtgagatat ggcgatgatg
     4981 aggtaagtat cacggttgcc acaactcgcc cagagaccat gcttggcgat ggcgctgttg
     5041 cagttaatcc aaaggatgag cgttataagc acctcatcgg taaatcagtt cttcttcccc
     5101 tagttaatcg catgattcca atcatcgccg atgagttagt tgaacctgac tttggaactg
     5161 gggcagttaa agtaacagca gcccatgacc caaatgactt tgagatggga atgcgccaca
     5221 acgttccgct tattgtgatt atgaatgaag ctggagtaat ggagggaact ggcactgaat
     5281 ttgatggcat ggatcgcttt gatgcaagag ttgcggtagt tgaaaaactt cgccagatgg
     5341 gaagagttgt ctctgagaag cgtccatata ttcacgcagt tggccattgc tctcgctgcg
     5401 aaacaacagt tgagcctcgt ttatctaagc agtggtttgt caaagttgct cctcttgcta
     5461 aagctgcagc agatgcagtt cgtgatggaa gagtaaagat tgatccagaa tctatggagc
     5521 caagatattt tgagtgggtc gataacatgc atgattggtg catctcgcgc caactctggt
     5581 ggggacatcg catcccggtt tggtatggcc cagatggaga tgtgattgtt gtaggtccaa
     5641 atcagagcgc tcctaaggga tatgaacaag atcctgatgt tttagatact tggttttcat
     5701 ctgcgctctg gccattttca actcttggtt ggccaaataa aactgatgat ttaaagaagt
     5761 tttatcccac cagcgtctta gttactggct atgacattct atttttctgg gtagtaagaa
     5821 tgatgttatt tggcttattt gctatggatg gagttgttcc atttaaagtt attgctcttc
     5881 atggtctagt tcgagataag catggcaaga agatgagtaa atctcgggga aataccgtag
     5941 atccattgga gtttatggat aagtatggct ctgatgcgct gcgctttacc ttggtgcgcg
     6001 gagctaaccc tggaacagat caagcacttg ctgaggagtg gatagcaggg tctagaaact
     6061 ttgctacaaa agtttggaac gctgcgcgct ttgccatgat taatggcgcg catgtaaatg
     6121 gcgagctacc aaatagatct gagctaaacc atattgatcg ctggatttta aatcgtctcg
     6181 atagcaccat cgctagcgct gataagggct atgaagcttt tgaatttgct aaggctagtg
     6241 aggagatcta tcactttgct tgggatgatc tatgtgattg gtatttagag ctagttaaag
     6301 agagctttgc caaaggcgga gagcaggctg ataagagccg cagagtctta ggtcatgttt
     6361 tagaccaact acttcgtctc atgcatcctt ctatgccatt tattactgaa accatctggt
     6421 gtgaattaac cgggggagaa tcacttgtaa ttgccaaatg gcccgcagta agtaaagccg
     6481 cgccagatgt tgcagccgat aaagtagttg gagatatgca ggttcttatc actgagatta
     6541 gaaggtttag aagcgagcag gggattaaga cttcggcgag aattaacgct caaatttcag
     6601 gtttagaagg agatctactt aattatgaag atgcagttag attcttagtt cgcctcgata
     6661 agccggcaag tgattttaaa gagagcgcaa ggattgaaat cggccaattt agtatcgcat
     6721 ttgaccttcg tggagcaatt gatgttaagg cggagcgagc aaggctaagt aaagatcttg
     6781 aaaatataaa gaaggatttg cagagcgcag ttgttaagtt ggaaaatgaa aactttatgg
     6841 caaaggctcc aatggaagtt gttagagaaa ttagagagcg tatggaattt tgcgagagtg
     6901 aaattactcg aatcaacact ctacttgctg cgcttccaaa agaataaaga tgagttctgc
     6961 tgaagatctt tcacgccttg atgccattga aaaagcgcta cttaatagat ggccagaaac
     7021 tagaattgct ccaacgcttg agcgaatctc aactttagct gattacctag gttcgcctca
     7081 gctctcttat ccaacaattc acatcgctgg aaccaatggc aaaaccacaa ctacgcgctt
     7141 aattgattca ctctgttttg aattggggat gcgcaccgga agatttacta gccctcatct
     7201 agagagtttc ctagagcgaa tcactatcaa cggccaatca ataactccag aatttatgat
     7261 tgcaacctat aacgatattg cgccatattt agagctagtt gattctaaga tgactaacaa
     7321 gctctcattc tttgaatcaa tgtgcgcact tgccttcgtt gcatttgctg aatttccagt
     7381 cgatgttgga atctttgaat gcggaatggg gggagagtgg gattcgacca atgtcattaa
     7441 tggctcagta aatgtcatta ctccaatagg ttttgatcat atggagtatc ttggcgatac
     7501 ccttgaaaag attgctcaaa ctaaatcagg aattattaag caaaactcat ttgttgttct
     7561 agctgaacaa gaaagtgatg tagcagctgt tttgatgcga gcttgcgcta aggctgatgc
     7621 cactccaatt aggtcaggta ttgaatttag cctcaaatca agggctctgg ctttaggtgg
     7681 gcagatgctg accatctccg gagtttatgg agagtatgaa gatttatatc ttccgctcta
     7741 tggtcagcat caagcaagta atgctgctac tgctcttgct gctgttgagg tctttgcagg
     7801 tgagacaaag cttgatgaag aaatagtgcg ccaggcattt gctaacgcca catcacctgg
     7861 aagatgtgag attgttggca gaaaccccac catcattatt gatgcagccc ataatcctca
     7921 tggcgcagaa tctttgaaaa agactatcag cgaggaattt gatttctcag cgctaattgg
     7981 agtcattgct cccatgggag ataaagatgt gaccggcatt cttgaagcac ttgagcctgt
     8041 tttagatcga gtgatagtaa ccagaaatag ctcgcatcgc gctgcagatg tagatgaact
     8101 aaaatcacta gctatggaga tttttggaag tgatcgagtc agtgcctgcg atgatttaga
     8161 gcaaggaatc actcaagcaa tagatatggc aagaactagt aatgccctta atgattcaaa
     8221 caccgcagtc ttaattgctg gatcagttgt tagcgctggt gaggctagag caataattcg
     8281 aagaaagggg atttaaaagc catgaagatg ctagccagaa gtgttttgat tatggagttt
     8341 ttaattattg gctttgctct tcttgttgct aaagatcttt caacgaagaa tagtctttac
     8401 tttggcgcgc taattgcgct cttggctttt ttctcatcag gccttttgaa atataaagtt
     8461 ggctgggctt taggttggct aacccagatt ctgctaattg catatggttt ctttatcccc
     8521 acgatgttta ttctgggatc cctatttctt ggactctggg tggcggcaat tatcttgggt
     8581 cgaaagggtg aggcggccaa agcggcccat atagccaagg cagaagggcc agattcgggt
     8641 agttagaaat gcccatagac ttaccccgtg agtaacgcat caacgccagc agagcgcacc
     8701 ctgattttgg ttaaaccaga tggagtgcgt cgtggtttag ttggtgaggt aatcagtcgt
     8761 attgagagca agggatataa aatcgttgca cttagaatgc taactgcaga taaagatctt
     8821 ctagctactc attacgcaga acatcaaggc aaaccgttct atgaaccgct tctagagttt
     8881 atgtcctcag gtccaatcgt tgcgatgatt gctgaaggcc agagagttat tgagggcttt
     8941 agatctcttg ctggagcaac tgatcccaca cttgcctcac ctggttcaat tcggggagat
     9001 ttagcccgtg atcaaggaac aaaagttgtg caaaatatcg tgcatggctc tgattcagtt
     9061 gaatcagcgc agcgtgagat aaagattttc ttccctaact tgtaaggagc gattaaatgg
     9121 caaagatgaa aaccgactgg tccttcctag gacgagatat ggctattgat ctaggaacag
     9181 caaatacctt ggtttatgtt cgtggcagag gtatcgttct taatgagcca agcgtggttg
     9241 ccgttaatca agatactggc gcagttcttg cagttggaac tgaagccaag aaaatgattg
     9301 gaagaacgcc aggaaatatt gtggccattc gccctcttaa agatggcgtt attgcagact
     9361 ttgataccac tgctctaatg attaagtact ttattcgcca ggttcataag cgcacctatc
     9421 ttgcaaagcc aagaattgtg atctgtgttc cttcaggaat tactggagtt gaacagcgcg
     9481 cggtcaaaga tgccggatat gaagctggag caagaaaggt ctacataatt gaggagccaa
     9541 tggcagcagc aattggcgct ggtcttccaa tccatgaacc aactggaaac atggtggttg
     9601 atattggcgg tggaactaca gaagttgccg tgatttcact tggtgggatt gtctctgctt
     9661 tatcaattcg tgttggtgga gatgagattg atcatgccat tattaattgg gtaaagaagg
     9721 agttctcgct attacttggt gagcgcacgg ctgaagagat aaagatggca attggatctg
     9781 catatcctct tcgtgatgaa ccagatgctg aaattagagg aagagattta gcaactggac
     9841 taccaaaaac aatcgttgtt tcatctgaag atatccgtaa agcaattgaa gaaccagtaa
     9901 atgcaattgt taatgctgtt aagggaaccc ttgataagac tccaccagag ttagcggcag
     9961 atttaatgga tcgcggaatc gtattaactg gcggaggagc cctacttaaa ggcttagatg
    10021 aaagattgcg ccatgaaaca aatatgccga ttcacattgc agatcgacca ctaaatgctg
    10081 tggttgaagg atctggaaag tgcgttgaag agtttgaggc gctagagaaa gttctgatct
    10141 ccgagcctcg ccgttagtta actcatggca actcgaggat ctaatagaag taggctcctt
    10201 tggatctcac tattggtgac ctcgcttttc attatcactc ttgatttaag gggagtaagt
    10261 cttctctcat ctcttagaag tggtactcaa agtgttttag ctcctgttga gcggctagca
    10321 aataatttct tctcaccagt tggtaacttt ttctccgagg tcagtaattt aggcagaagt
    10381 aaagcaaaaa tagagaagtt agaggaagag aatcaagagt taaagagcca agttattttc
    10441 aatgaaaata cgctagcgca attagagtcg ctcaaaggcg ttttggatct ggctggaaaa
    10501 gctcgctata agacagtgtc tgcaagagtg attagcaaag gcggaacggg aacatttagt
    10561 gaaactatag ttttagatat tggcagtgat tctggagtaa gacgagatat gacagtcatt
    10621 gcagcaggcg ggcttgtcgg agtagttaaa tcaactactg cttcatcatc aattgtgctt
    10681 ttaatgaatg acccatcttt tcgaattggc gtaagggttg ctggaaagca agatatggga
    10741 attctgcttg gacaaggcga taagagctac tcactacaga tgctaagtgc aacctcagca
    10801 attagcgttg gtgatgtctt gctagcacgt ggaagttctg gagataagcc ctttgttcca
    10861 ggagtgccag ttggaagaat tagttatgtt gaaaataccg ttggccaatt aacaaaagag
    10921 ggaagggtta atggatttgt tgacctaaat tctctctcag tggtgagcgt agtcttatca
    10981 gcagttgccg gagatcctgg cgatgcctta gtgcctcaag ccccaaggcc tgctccaaca
    11041 gttacggtat ttgtaacacc tagccccaca ccaaaggaat aaggctatgg agattaatcg
    11101 cctgccaaaa gtcttgttaa ttttcctatc actcttcctt ttgcaagagg ctttaattaa
    11161 tcagattgat tttttagttg ccggcttttc actctatctt gcttttttta tggcctggat
    11221 aattcaagat cagagaaata gcgctgtagt tactggattt attgctggtt tgattctgga
    11281 tctatcgcca actcttgaag caccttttgg gctttggact cttgtgctta cctcaatgag
    11341 ttatctgcta gcaagtaatg ttagagcggc gctagatgct gagttaagcc cgctagtaat
    11401 gctaagtgtt gcagtcgtag caagctcatt tgccttaatc ctcttcttat tatttggcgc
    11461 gatcttgggt caagaagttg gctctgcctc atatttaatt agaagtattt caggaaatgc
    11521 tctctggagc gccctacttt caccacttta tatcccgctt gcaatgaaac tttataaagt
    11581 aacccttacc gcaaggcagc gataaatgag agatcgctca gcgctaaccc ttctggttgc
    11641 ccaagtattg gttatctcgc tgatgctctc tttatttggt cgactctttt atcttcagat
    11701 tgcagacggt ccgcgctatc aagaagcggc acttgatatt caaagcagag atatcgtcac
    11761 tccagctctt cgtgggttga ttgtcgatca tgaaggaaac gctcttgcaa ccaataaagc
    11821 agggctaatg gtcacagctg atcgatcagt tctagataag ttagaagata aaggagtttc
    11881 agttttaact agagtggcag aggttttaaa actggaatat agcgatatct acaccagaac
    11941 taggctctgc ggtgagctag caagtggtga gcgaaatgga tgctggaatg gaaatcgcta
    12001 tcaaccaatt ccaataacca aagatgcaag tgagaatcaa gtatttacta ttctagaaaa
    12061 atcggacctc tttcctggaa tcgatgctaa accagtttca atccgctcct atccatcact
    12121 tgctgggcag aaagctaccc atgtgctggg ctatttgggg cctattaccg aaaagaattt
    12181 aaataacgaa gaaggaaaac gatattatcg aaatgaatta attggtaaag ctggaatcga
    12241 attgctctac gatgaacaac ttcgcggagt tcctggaatt aggactgtca ttgttgatcg
    12301 taaagaaata gttagacaag aatcactcaa tagagcgcca gttcctggta atcatctagt
    12361 tttaaacctg aatgctaaat tacaagctgc agttgaagta gaactcaaag atgcagtctt
    12421 tcgcgcaagg gctaatggat atcgcggaga ttctggggct gccattgtta tggatgtgaa
    12481 gactggcgaa gttctagcga tggcctcata tcctgattat gatttaaata tttgggaaaa
    12541 tggaatcact gttaatcagg caagagatct ctatagtgaa aagagcggag taccagcgct
    12601 atcacgggcg atccaaggtg aatttgctcc agcctcaaca tttaaagtcg tttcactctc
    12661 tgcagcagtt gatgctggat atagcctgaa aactacatat gagtgtccag ctgaagttca
    12721 aatcggtgat agaactttta agaattttga tagcaaggct gccggtcgaa tcagtatgac
    12781 aactgctatg gcaatttctt gcgactcaat ctggtatcag attgcctatg acgaatgggt
    12841 tcgagatggc gggttaaagc caaaaccaag cccaaatgat tacttcttcg attcagcaag
    12901 gggatttggg gtagggaaaa agaccggagt tgatcttcct tccgagttat cagggcgact
    12961 tccagatagg cagtggaagt taaactggta tgaaaagaat aaggatttct actgtaatta
    13021 cactgaaaag gctaagaaat ctcagctcac gccatatctt attgagattg cccgagagaa
    13081 ttgccttgat ggcgacaaag tcagagcggg agatatggtc aacttctcaa tcggccaggg
    13141 agatactctg atgacaccat tgcaattagc tgttgcctat tcagctcttg ctaatggtgg
    13201 caagttaatg gtgccaaagg ttgccaaggc aattgttgag ccaagcggca gagtagtaaa
    13261 ggaatttaaa ccagagatca gtggtcgcct tccaattaag aaatcaactt taaaatttct
    13321 gcaaaattca cttcgagcag tggttacctc aggcactgcg actggagcat tctctggagt
    13381 tggagttgag gtaagtggaa aaactggaac cggtcaggtc tttggcagaa atcttgatgg
    13441 ctcaagtaaa gatgacactt cttggtttgc ctcttttgct ccaagtaaga aaccagaata
    13501 tgccgttgtt atgatggtaa gtcagggtgg ttttggtgcc agcactagtg gtgttggcgt
    13561 gagaaaaatc tacgaacata tctttggcac taatggccgc gtagcaatat ttcctaatgg
    13621 aacaccgaaa aaacttccaa gaattgattc tgcgaaggta gcgctgccat gaaaatttcc
    13681 ctaaaaggtc agggcaaaaa aggattcttt gcaggcacag atcaagtttt gaacttggca
    13741 gtgtttatgc ttctcttgat tggcattgtc ttggtttatg cagcgactag agattggttt
    13801 gctgctaatg gactagatcc tgagtactac ctaaagcgcc agatagtaaa tgtcattatt
    13861 ggtattgccc tggcctatgg cacaactctt gtggattacc gccttcttcg ggcctacaca
    13921 ccatttctct ggggctttgg cgtattaggt cttattgcag tattaattcc tggacttggc
    13981 tcaacagtta atggcgctcg ctcttggatt tcatttccag ggggcttgca atttcagcca
    14041 gcagagctag caaagatttc aattattgtt ggaatggctc ttattctctc agagcgcatg
    14101 gcaggagagg atcgaccaag tgataggcag gtcttgcaag cgctaggtgt tgcagcgctt
    14161 ccaataattt tgattgtagc tcagccagat ttaggaactg tgctaattat ctctgcagcg
    14221 gtgataacaa tgattgcact ctccggcgca tctctttatt gggtgattgg acttctaatt
    14281 gctggagttg ttggcgtcta tattgcaatt gctagcgggg aggtaagtga gtatcaatta
    14341 aatcgcctaa aatcttttgt tgatccttca gcagatccgc aggcaagtgg ctatcaatta
    14401 agacaggctc gtatcactat tggttcaggc ggcttacttg gtaaaggttt gtttgatgga
    14461 ccgcagacca atggaagatt tgttccagag caacagacag attttatttt tacagtagcg
    14521 ggcgaggagt taggttttct tggttcttca tttattttgc tactttattt catagtgttg
    14581 atgcgggcct ttgctatcgc aagagccacc acagatctct ttggcaaaat ggtctgtatc
    14641 ggtgttgttg cttggttctc atttcaaatc tttgaaaata ttggaatgac aatggggctt
    14701 atgccaatga ccggggttcc actcttattc ctgtcttatg ggggctctgc aatgtttgcc
    14761 aatttgatcg ctattggcct attgcaaaat gtctatcaaa ggacgcgata agagctgctt
    14821 tttgcgccta attaggcgaa ggctttaact atctgccata cttgcccagt tcgaagtcgc
    14881 ctcacgttct tgaggtcgcc aagaccgctc tttaggtgcg gtcggcagat ccggaaaaaa
    14941 aggcgccacc acagattttt tagcaaatgc ttttaacaaa agcgtttatg aaaacttata
    15001 agaaggattt gatcgtatgg ccgaagagcc aaaaaaagtg cgtaagcgcg ctgtaaagaa
    15061 aagcgcgagt aaagcagtag agcagagcga gagcgctcct gctgctaaag agaagaaatc
    15121 aaaagccggg gtaatcccag ctccaatatt tcaagcagct cccgttgctg ccgccccaaa
    15181 ggcaaagcca gcaacaacaa aacctgctgc aaagagctca gctgaagtct ctggcgagag
    15241 tgaggcggag agagaaaatc gccgacgccg tcgtcgcagg ggaggccgtt ctcgtcgcag
    15301 gttagaaaaa gatgaaagcc ctacaactag ttcagagtta agccagggcc caagtagtga
    15361 ttctgaaggt agcgttggat ttaagaaacg ccgccgccgt cgtcgctcag gggctgaagg
    15421 tgtagctcca ggtgaaaaga ttgaagaaga tggcgtatta actgtagtta aagtacgtga
    15481 gcctcgtccg gttcaggaaa agccggttag agagaagaag agctttgatc gcccacctcg
    15541 tcgctttaaa gatcgcgata gagatagagg acgagataaa cctagatata acgattatcg
    15601 tgaaaatcgt cgtcgcggca caatccttac cgatgctgaa tttcttgcaa gacgtgaatc
    15661 tgtagatcgt gagatgttag ttcgccagca aggcgataga actcagattg caattttgga
    15721 agacaaggtt ttagttgagc actacgtcaa tcgcaatagc aatatctcct atgttggaaa
    15781 cgtctatctt ggaaaagttc agaacgtttt accttcaatg gaagctgcct tcgttgatat
    15841 cggaaaggga cgaaatgcag ttttatatgc tggcgaggtt aattgggacg cagcaggtct
    15901 tgctgataat caagagcgaa agattgagaa agttctaaaa actggtcaat cagtattagt
    15961 tcaagtaact aaagatccta ttggccaaaa gggtgctcgt ttaactagtc agatctcact
    16021 tcctgggcgt tatttggttt atgtaccagg tggaggaatg agcggaatct caagacgact
    16081 tgcagatact gagcgctcac gcttaaaaga aattcttaaa ggtctaatca ccgatgaaga
    16141 gggcgtaatt gttagaactg ctgcagaggg agcaagcgat gaagagctta ctcgcgatgt
    16201 aattcgccta aaggcgcaat gggatgacat taaggcaaag tctgaaaaca ccagcacttc
    16261 agccccatca cttctctatg gtgagcctga tctaacggtt cgagtaatta gagatatctt
    16321 caatgaagat ttccgcaagc tcacagtgca aggaagcgat gcttgggatg atattaataa
    16381 ttatcttgga catattgccc ctgagctttt aacaaagatt gaaaaataca gccaaggcaa
    16441 tgatctcttt gctgaaaata gaattgatga gcagttagct aaggcacttg accgtaaggt
    16501 ctatctgcca tctggtggtt cattagttat tgatcgcact gaggctatgg tggttattga
    16561 tgtcaatact ggaaagttca tcggtaaggg tggaaatcta gaagagactg ttactaaaaa
    16621 taaccttgaa gcagcagagg aaattgctcg tcaattaagg cttcgagatt tgggtggaat
    16681 tatcgttatc gactttatcg atatgatttt agaatcaaat cgcgagcagg tactacgcag
    16741 attagttgag tgtttaggaa gagatagaac taagcatcaa gtagctgaag taacatcact
    16801 tggtttagtt caaatgactc gaaagagagt tggccaaggg ctaattgaag ccttctctac
    16861 aacttgtgat agttgtggtg gtcgaggcat tcatgttcac atggagccag ttaataagaa
    16921 gagccttgat cgtgaatttg ttccagcaaa ggtcaataaa gaggagaacc tctcatctga
    16981 tgattcagaa agtgatgaga aagcaagcct ggatgaaaat ctgactccgg ccaaaaaaga
    17041 tggagattct ggccaaaaga aaggctttgg taggcgtaaa cgcagggctg ccagctctgg
    17101 agttgtaacg gcctaactca gtttgaccct agataggctc aatccgtaac ctatgcccct
    17161 gccccgataa gggtctttgg caagagaaga taaagaaatg gaaggaccat tgtgtacgca
    17221 atagttaaag ctggcggtcg ccaggagaag gtcgctgtag gcgatactct ctttgttgat
    17281 cgtattgatg cagctgctgg ttccaccgtt tcatttcctg ccgtattagt tgtcgatgga
    17341 tcagcagtaa caagtgatcc aaagctgctc tctggagtta aagtaactgg agaagttatt
    17401 tccgaggtta agggcccaaa gattcatatc ttgcgcttta agaataagac cggctatcgc
    17461 cgtcgtcagg gttttcgttc taaaaacact cgcgtgaaaa tcactgcgat taatggtgtg
    17521 aagtaaaggg gttggcataa atggcatcga aaaaaggcgt ctcctcaacc cgaaatggtc
    17581 gcgattccaa tgcacaattt cttggaatca aaagatttgg tggtcaggta gttaacagcg
    17641 gcgagattct cgttcgccag cgtggcacca agttccaccc aggtaaaaat gtaggaatcg
    17701 gaaaagatga cacgttattt gcactagctt caggttcagt ggagtttggc agtgctcgtg
    17761 gtcgtcgcgt tgtaaatatc gtcccggttt cctaagcaac ggactttaaa aaattgcggg
    17821 tccgtgcata cgggcccgct ttttacttat ccacctaatt tatagaaagg caagagtcag
    17881 atgacaacct tcgttgatcg cgtgactctt catgccgcag caggaaaagg tggagatggt
    17941 tgtgtctctg ttcaccgtga aaaatttgtt ccacttggtg gtccagatgg tggaagcggt
    18001 ggtaggggcg gcgacattat tttggttgtt gatccaagtg ttactacctt gcttgatttt
    18061 catcattcac cacatcgcag cgctagctcg ggacgacagg gatatggtgc gcttaaaaat
    18121 ggccctgatg gcgaagattt aattcttggt gttcctaatg gaacagttgt gatgaataaa
    18181 gaaggccgca tcttggcaga tctagtaggt aatggaacaa gatttattgc tgctaaaggt
    18241 ggtttaggtg gccttggcaa taatgcactt gcctcaaacc ggcgcagagc tccaggtttt
    18301 gctctccttg gtgaacctgg cgaaaaacga actttaattt tagaacttaa atctgttgca
    18361 gatattggtc taattggtta tccaagcgca ggtaaatcat ctttagttgc agctatttct
    18421 gctgcaagac caaagattgc tgattatcca tttacaacac ttgccccaaa tcttggagtt
    18481 gttcaggcag gagaggcgag atttactgtt gcagatgttc caggtttgat accaggggct
    18541 tcgcaaggaa aaggtcttgg cttgcaattt cttcgccatg ttgagcgctg cgcagcttta
    18601 gtacatgttc tcgattgtgg aacgctagag accgagcgcg atccagtgag agattttgaa
    18661 gtaattgaag atgagctaaa gaagtatgca gaggacttaa gcaccagacc tagaatcatt
    18721 gctcttaata aagttgatct tcctgatgct aaagcaatgg cagatatggt cgagccaacg
    18781 ttaaaaaaga tgggatatga ggtttttcag atctcagctg catcaagaat tggacttgaa
    18841 caacttctat atgccatggc taagttagtt gcagaacata gaaagcagga gagcgcacaa
    18901 gagaaggtaa agattgtgct tcgcccaact ccaatcgatg atgtcggctt taaaactatg
    18961 gctaatgatg atggctcgtt taccgttatt ggttcacgcc ctgagagatg ggttcatcaa
    19021 acaaacttta gtaatgctga agctgtcgga tatcttgcag atcgcctagc aagtttgggt
    19081 gtagaaaaag aactctttaa attaggggcc aaacaaggcg atgaagtacg tattggtcta
    19141 ggtgatgacg cagtgatctt tgattgggag ccaactattg aggcaggcgc agaacttcta
    19201 tcaggacctc gcggcgatga catgcgtatc ccgcgcggat gggagaagtt tgatgaagag
    19261 gcaattgatc aattagatga tgaagaatta gcccagcaat gggagtacaa ggtggaagat
    19321 ccaacagatc caggagtaga aatggaggag cagaaatgaa tcgcgatgca gttattaaag
    19381 caaagcgaat agttatcaag attggctcct caacacttac tggagcagat ggcgccggcc
    19441 ttgatagcgc tgccgtcact aaattggcag cagcagttgc tgaattaaaa gagcagggta
    19501 gagaagttgt tgttgtttca tcaggagcaa ttgctgcagg tcttgcccct cttggactag
    19561 gcaaaagacc tgccgatctt gcaacgcaac aagcagcagc atctgttggc caggggctat
    19621 taattcatag ctataccgaa gcgttaagaa aacacaaaat cattgcttcg caagtgctat
    19681 taactattga agacattgtc cgtagatctc attatcaaaa tgcgcagcgc actctcttta
    19741 aattacttgc gctcggcgtt gttccaatta ttaatgagaa tgattcagtt ggaacacaag
    19801 agattagatt tggtgataac gatcgaatcg ctgctttagt ttctcaccta attggagcag
    19861 atttattggt gctagttagc gatgttgatg ctctctatga tgctaatcca aaagaggctg
    19921 gagctaagaa gatttctcaa gttgattccc ttgctgagtt ggcagatgcc caaattgggg
    19981 gagctggatc taaagttggc agtggcggta tggttacaaa gattgaagct gcgcgaattt
    20041 caacccaagc tggaattcca atgttattaa cctgccttgc tgatgtttcc aaagtattgg
    20101 ctggatccga acttgggaca tactttgttg catcaacaga taagaagtct tctcgtctgc
    20161 tctggttagc gcacgcggca agtagcagtg gaagattaat tatcgatgct ggcgctgtta
    20221 gcgcgctgcg tgagcgtggc acctcacttc ttcctgctgg agtaaaagct gttgaagggg
    20281 aatttagctc aggagatacc gttgagataa cggccgaaga tggcagcgcc attgcccgcg
    20341 gattagttgc ctttgattct tcagaaattc cagcaatgct tggacgctcc actaaagatc
    20401 ttgcgaaaga attagggcct gaatatgagc gagagttggt ccaccgcgat gacatggtgc
    20461 tgttatgagc aatgatcaat tagttgcaga tttagcagat aaggcaagaa aagcatcaaa
    20521 gactttaatc actgctggct atagcaagag acaaggtgca ctgcttgcaa ttgcagatgc
    20581 acttgaggcc aatatgggtg agatccttga agccaataag ttagatatgg aacgcggaaa
    20641 agctagtggc ctcaattcat cccttctaga tcgcctagct ctaagcccag agcgaatcaa
    20701 agacattgcc tcaggggcgc gccaagtagc agcacttgat gacccacttg gaatcgtttt
    20761 aaggcagcga acgctaagta gtggtttgaa actcacgcaa ataaccgtgc catttggggt
    20821 tattggaatg gtttatgagg ctcgtccaaa tgtaactgtt gatgctgcag taattcttct
    20881 gatgtcagga aatgcagccc tacttcgtgg ttcatcatct gctaaagcaa gtaatgaggt
    20941 cttggttaaa gtaatgagag gcgcacttga aaaaggagat atttcaagtg aggtaattca
    21001 attaattcct tcaagtgatc gtgatactac tagggccctt cttcatgctc gaggcaaagt
    21061 ggatttagtt attccaagag gaagcgcaga attaatccgc atggttgttg atgattcaac
    21121 tgtgccgact attgaaactg gagctggagt ctgtcatgtt tatattgata agagcgctga
    21181 cttttccaaa gctcttccaa tcgtaattaa ctcaaagact catagaccta gtgtttgtaa
    21241 tgctgcagag actcttctag ttcatcaaga gatagccaaa gactttctac cgctagctct
    21301 taaagaactc catcaggcgg gggtaattct gcattgcgat caaataagcc ttgaggttgc
    21361 taactcactt tcaattccag catcccttgc cactgaagag aattggtcta cggagtacgg
    21421 cgtattggag atgaatgttg ctgttgtgcc tgatttagat agcgctgctg ctcatatttt
    21481 gaagtatgga accaatcaca cagaggcaat tgtcactgaa tccaatgaga gcgcagaaag
    21541 atttattgct cttgctgatt gcgcagcagt tatggtcaat gcctctacta gatttactga
    21601 tggccaagaa atgggatttg gagcagagat tggaatttca aatcagaagc ttcatgctcg
    21661 aggtccaatg gggcttgagc agatgactac caccacatgg attgtgagag gagatggcca
    21721 gatccgaaag taattcgctg gcagcgatag ctcttcggta aggtgaccta tatgtccaat
    21781 atctcgcgcg atgatgtggc aaatctagcc aagttagctc gcattgattt atcagaagat
    21841 gaattggctg gtcttgctaa agaactctcg gtaatccttg acgccgttgc tcgagtacaa
    21901 gaggttgcag gcgaagatgt tccaccaact tctcatccac ttccattagt taatgtcttt
    21961 cgtgaagatg tagtccgtcc aagtcttact ccacaggaag ctctctctgg tgctccagag
    22021 caagaggagc agaggtttaa agttccgcag attttgggtg aagagtgatg attaataaga
    22081 gcgcagttga gatggcagag gcgttaaaca agggcgaaat cacaagcgtt gaactaacta
    22141 aggcgcatct aaagcagatt ggtgaagttg atggacaggt tcatgctttt cttcatgttg
    22201 atacgcaggg agctttggat caggcaagcc aagtagatgc aaagagaagt gctggtgaaa
    22261 aacttcctgc cttagctgga gttccactag cccttaaaga tgttttagtt caaaaaggaa
    22321 ttcccaccac ctgtggctca aagatgttag agggatggcg ccctccatat gattcaacgg
    22381 ttgttagaaa gttaaaagaa aacggcatag ttattttagg taaaaccaat atggatgagt
    22441 ttgctatggg ctcatcaact gaaaattcag cttatggtcc aagccgaaat ccttgggact
    22501 taactcgcat ccctggcgga tcaggcggcg gctcatctgc ctctctagca tcatttcaag
    22561 cacctcttgc aattggaact gatactggag gatcaattcg tcagccagct gcagtaacag
    22621 gaactgttgg agttaagcca acttatggcg gagtttcaag atatggctta gttgccttct
    22681 catcatctct tgatcaagca ggaccgtgcg ctagaactgt tcttgatgcg gcatatcttc
    22741 atgaagcaat tgccggtcat gaccctctag attcaacttc tattaacgcc ccagttccag
    22801 cagttgttgc cgctgctaag gatggaaata taaagggaat gaagattggc gttattaaag
    22861 aacttcaagg agatggttat caagctggag tattaaagtt atttaatgaa tccctagaaa
    22921 ttctggcatc acttggcgct gagattgtag aagtctcctg taaatacttt gattatgctc
    22981 tagcagctta ttatttaatt gcgccaagcg agtgctcttc aaatcttgct cgctttgatg
    23041 cgatgcgtta tggaattaga aatggtcagg gctctgctga agaggtcatg aatgccacaa
    23101 gacagattgg ttttggcagg gaagttaagc gcagaattat ccttggaaca tatgcactct
    23161 caagtggtta ttacgatgcc tactatggaa gcgcacagaa ggttagaact ctaatctcac
    23221 gcgattttga aagcgctttt aaattagctg atgtcttagt ttcaccgaca gcgccaacga
    23281 ctgcatttaa gattggcgag aagtccaatg atccaattgc gatgtattta aatgacattg
    23341 caacaattcc aactaacctt gctggcattg gtggtatgtc cctgccatca gggctagctg
    23401 ctgaagatgg gctaccagct ggttttcaaa taatggcccc agccatgaaa gatgatttga
    23461 tgtataaagt cggcgctgcc cttgaagccg agctaaatac taagtggcag ggaccaattc
    23521 taaattctgc tccaaagcta ggagctaaat aatggctctt aagtatgaag atgtaatcgc
    23581 aaaatatgag ccagcacttg gtcttgaggt tcacgttgaa ctaggcacag catcaaagat
    23641 gttctgtggc tgcaatacag tctttggagc agctgccaat actcaaacct gcccggtctg
    23701 cttaggcctt ccaggcgcgc ttcctgtagt taatggcaaa gccattgaat acacaattgc
    23761 aataggacta gctcttaatt gctctattgc gccatatagt cgctttgcta gaaagaatta
    23821 cttctatcct gatatgccaa agaattttca aacttcacaa tatgacgagc caatctgcac
    23881 caatggatat ttagatatca caattgatac cgatgaaggt agcaaggact ttagaattga
    23941 aattgaacgt gtccatatgg aagaggacac tggaaagtca ctgcatatgg gaggggcaac
    24001 tggtcgaatt catggggctg attactcact tcttgattac aaccgagccg gaattccttt
    24061 agttgagata gttacaaaag ttgttaatgg ctgcggcaaa tacgcaccag aagttgccag
    24121 agcatatgtc tcagaacttc gagatatttt gcgcggcctt ggagtaagcg atgtgaagat
    24181 ggagcaggga tcactgcgct gcgatgctaa cgtctcactt cgtccaattg gttctgagaa
    24241 acttggaaca agaagtgaga ctaagaatgt caactcactt aagtcagtag agcgcgcaat
    24301 taggtcagag atgattcgcc atgctgaact tctaaattcc aatcagcgag taatacagga
    24361 gactagacat ttccatgaag ataccggact aacaacttct gggcgctcaa aggagcaagc
    24421 cgaggattac cgatattttc cagagccaga tttggttcca attgctccaa gtaaagagtt
    24481 aattgcaaag attgctgcga cccttccaga aaaaccctca gttagaagaa agaaattaca
    24541 agaggcttgg caggtgccag ataaagagat ggcagcgatg attaatgctg atgttctaga
    24601 tttagttgaa gcaactgtga aattaggagc agatccaact cgcgctcgct cttggtggtt
    24661 aggtgaaatt gccagaattg ctaatgagaa ggcggttgct gccgcagatt tagctatcag
    24721 cgctgcagat gttgctgaag ttatcgccct aattgttaag ggtgagctaa ccgataaatt
    24781 agctcgccag gttgttgagg cgctaattaa tggcgagggt agcgtggcaa aaataattga
    24841 aactcgtggc atcaaagttg tctccgatga ttcatctcta atggcagcaa ttgagcgagc
    24901 aatcgcagct gctccagatg ttgcggaaaa ggttagaggc ggaaatatcc cagcctcagg
    24961 agctttaatt ggcgcagtaa tgaaagaatc aaagggtcaa gctgatgcag cgaaggtccg
    25021 cgatcttctt ctaaagcatt taggtcaggg ataagtagga ttaactcatg acaaatccaa
    25081 tgaagccgcg ttctggactt gtaacagatg gtcttgagcg cgctcctgct cgcggaatgc
    25141 ttagagcggt aggaatgaaa gatgaagatt gggttaagcc acaaattgga attgcatctt
    25201 cttggaatga aatcacgcca tgtaatctct cgttagatcg tttagcaaaa gcatcaaagc
    25261 aaggagtcct tgatgcaggt gggtttccaa tgcagtttgg caccatctca gtctctgatg
    25321 gcatatcaat gggccatgag ggaatgcact tctcattagt ttcaagagag gttattgcag
    25381 actctgttga aacagttatg caggcagaga gatttgatgg catggtcaca tttgctggtt
    25441 gtgataagtc tcttccagga atgatgatgg cagcagcgcg tcttgatgtt gctagcgtct
    25501 ttgtttatgc cggctctact cttccaggac aagtcgatgg aaaagatgtc accatcattg
    25561 atgccttcga agcagttgga gcatgcgcca gaggcttaat ttctcaagag caggtcgata
    25621 aaattgagcg cgcaatctgt ccaggtgaag gtgcctgcgg cgggatgtat accgcaaaca
    25681 ccatggcaac agttggtgag gcaattggtt tatcacttcc tggctctgct gctccgccag
    25741 cagttgatcg caggcgtgat agttatgctg ttaagtcagg agcagcagtt gttgagctca
    25801 taagagcagg aataacgaca agagatattt taagtaagca agcatttgaa aatgccatta
    25861 ctgcccttat ggccctcggt ggatcaacta acgctgttct tcacttacta gcaattgccc
    25921 atgaagcaaa ggttgattta accttaaatg atttccatcg cattggctca aaggtcccac
    25981 tacttggcga cttaaaacca tttggcagat ttgttatgag tgatgtcgat aaagtcggag
    26041 gaattccggt gatattaaag gccctacttg atgccggcct acttcatgga gattgtttaa
    26101 cagttactgg aaaaactatg gctgagaatt tagctgatat caatccacca gatcctgatg
    26161 gcgaaatatt aagagcggta aaaagtccga tgggtgtaag tggcggaatc acaattttaa
    26221 gtggctcact tgctaaagat ggcgcagtga caaaggttgc tggagttgga gttgactcct
    26281 tcgaaggacc tgccagagtc tttgatagag agcaagctgc gatgcaagcg ctagaagatg
    26341 gaactattac agcaggtgat gttgtagtga ttcgctatga agggccaaag ggtggtcctg
    26401 gaatgcgcga gatgttaatg attactggag caataaaagg cgcgggtctt ggaaaatctg
    26461 ttctcttaat taccgatggc agattctccg gtggcacaac cgggctatgc gtgggacatg
    26521 ttgcaccaga ggcaagtgat ggtggagcaa ttgcacttgt taaagatggc gatcgaattc
    26581 ggattgatat caatgcaaga acgctagatt tattagttga tgaaagtgag ttagcaaaga
    26641 gaagagagag ctttaaaccg ctaccaagta ggtatacctc tggggtattg ggtaagtatg
    26701 taaagcttgt gggatcagca tcaaagggcg cggtctgcga ctaagagggg attaatcccc
    26761 gttcatattg tgagatggcc gtcttaaggg ttgactcaaa ctttttatca ggggatactt
    26821 atccacatgt cagcgaaatc aacttctgta atagcacttg tttcagtggt gattcgctgc
    26881 gcgtgaacta gataaaagtt cacgcgcacc ctcaggaaac tgagggtttt tgcattagtg
    26941 ggcctaaaaa gaattggaag aaaggagtga accaagatgg caagtgagat aacaggggct
    27001 caagccctcg ttaacgcgct agaagattct ggcgttgatg tcatgtttgg tattcctggt
    27061 ggcgcaattc ttcctgccta tgatccaatc tttgattcaa agattcgcca catcttagtt
    27121 cgccatgaac agggagcagg tcatgctgca actggatatg cccaagttac cggccgcgtt
    27181 ggggtatgca ttgcaacttc agggccagga gcaacaaatt tagttacacc aattgctgat
    27241 gcgcagatgg attcagttcc aattgttgct atcactggtc aagttccatc agctgctatt
    27301 ggaaccgatg ctttccaaga agctgatatc cgcggaatca cgatgccggt aacaaaacat
    27361 aattacttga taactgatcc tgctcaaatt cctcgggcaa ttgctgaagc tttttatatt
    27421 gcatcatctg gaaggccagg accagttcta gttgatatcg ctaaagatgc gctacagaag
    27481 atgactagct ataactatcc aaagaagtta aaccttctag gttataaccc gcagattgaa
    27541 cctaactcag aggcaattcg agatgctgca tcacttattg ctcaatcaag taaaccaatc
    27601 ttctatatcg gcggcggagt aataaaggcc aatgctgcaa aagatcttct tgtgttagca
    27661 gagctagttg gagctccagt tgttacaact ttgatggcaa gaggggcatt tcctgatagc
    27721 catagccagc atcttggcat gccaggaatg catggaacag ttgctgctgt tactggcctg
    27781 cagaaagcag atttacttat tactttaggc gctcgatttg atgatcgagt aactggaaaa
    27841 ctctctacat ttgccccaaa tgccaagata attcacgccg atattgaccc ggcagaaatt
    27901 ggtaagaatc gctatgccga tgtgccaatt attggcgacc ttggtccaat tattagagcg
    27961 ctaattcccg ctgttaaagc tgagtttgct aagaataagg ctgatatcac ttcgtggtgg
    28021 aagcagatga attcgctacg ccagaaatat ccccttggat ataacgaacc aagtgatgga
    28081 tcgctatcgc cacaatatgt aattgagcga atcggagcaa ttaccggccc tgatggaatc
    28141 tatgttgcag gtgttgggca gcatcagatg tgggcatcac aatttgttaa gtatgaaaaa
    28201 ccaagaacct ggctaaattc tggtggcctt ggaactatgg gttatgcagt tccagcagca
    28261 atgggggcaa aagttggcgc gcctgatact ccagtttggg caatagatgg tgatggttgc
    28321 ttccagatga caaatcagga attagtaacc tgcgccctga acaatattcc aatcaaagtt
    28381 gctgttatca ataatgagag ccttggaatg gttcgtcagt ggcagactct cttctataaa
    28441 gagcgttact caaatactga tctgcactcc aagagaattc ctgactttgc aaaacttgca
    28501 gaagcgatgg gctgtgttgg tcttcgctgt gagtcaaaag gtgatgtcga taaaatcatc
    28561 aaagaggcaa atgcaatcaa tgatgcgcca gttgtagtgg actttagcgt tcatagagat
    28621 gcgatggtct ggccaatggt ggcggcagga acttctaatg atgatatttt gattgccaaa
    28681 gaaatggcac ctgactggga ttcacaggag ttatagccat ggcccaacac actctctctg
    28741 ttcttgttga aaatagccct ggtgttctag ccagggttgc atccttattt tctcgccggg
    28801 gatacaacat tgattcactt gctgtggggc caaccgagag tcctgagatt tcgcgaatta
    28861 ctattgtttt aaatcttgag ggccatgcat tagatcaggt cagcgcccag ctatttaaat
    28921 tagtcaacgt gatcggaatt caagagatgg aagataagaa ctctcatcaa agagagctat
    28981 taatggttaa agtaaattgc actggagata atcgctcaaa gattgaggca gttgtgggca
    29041 aatatcgagc ctcaattgtt agccaggctt cctccagcct taccattgag gcgataggaa
    29101 gtagcgctga gattgcagcg ctattaggtg aactatcaag ctttggcatc aaagagttag
    29161 ttcaatcagg tcttattgcg ctagaaaatg gcgatgcgac tttggctgaa agaactttgc
    29221 aagaaactgg actagcttct tcatcgacgc atagccagac cgaggactac caaaactaaa
    29281 ctagaaagca ggagaacgtg gcaactattt atcatgacga agatgcagac ctttcggtaa
    29341 tacaggggcg aaaagttgcg gtcattggat acggttcaca aggacatgcc catgccctaa
    29401 acctgcgcga tagtggtgtt gatgtaagag ttggattagc cgagggttct aaatcccgcg
    29461 ccaaggcaga agaagagggc ttaagagttc tctcggttgc agaagcagtt aaagaagcaa
    29521 cagtaattat gctccttgca cctgatcata agcagcgcca tatttataaa gaatctatcg
    29581 aacctaatct aaaagctgga gacgcgctct tctttggtca tggatttaat attcgctttg
    29641 gatatatcaa gccgccagca aatattgatg tttgtatggt tgctccaaaa ggcccaggac
    29701 atctagtaag acgtgagttt gctgcagggc gcggagttcc agatatcgta gctgttgagc
    29761 aagatgcaac tggatctgct tggccactta ctctctctta tgcaaaggcc atgggaggtt
    29821 taagagctgg cggaattctt acaaccttca ctgaggagac tgagacagat ctatttggcg
    29881 agcaatcagt actctgcggc ggagcatctc aattagtgca atacggtttt gaagttctca
    29941 ttgaagctgg atatcagcca gaggttgcct actttgagtg tctccatgag ttaaaactca
    30001 tcgttgatct tatgtatgaa ggtggaattg ctaagcagcg ttggtcagtt tcagatacag
    30061 ctgaatatgg cgattacatc tcagggccaa gagttattga tccaagagta aaagagaata
    30121 tgaaggcagt cctaggagat atccagagcg gcgcatttgc taagcgcttt atcgaagatc
    30181 aagatgctgg ggctccagaa tttaaatcac tgcgcgctaa aggtgagtct catccgattg
    30241 aggaagttgg cagaaatcta cgcaagatga tgtcttgggt taaagctgcc aataaggatg
    30301 attacaagga aggatctgct tcgcgtggct aaacctaaag ttctaattgc tgaggagcta
    30361 agtccagcaa ccctagatgc cctaggacca gagtttgaga ttatcaactg tgatggggca
    30421 aatagatctg agttactggc ctgtctgtct tcaggagttg atgcagtttt aattcgctct
    30481 gccacaaaga tggatagcga agcaatctct gcagctaaag gtttaaaggt cattgcaagg
    30541 gctggtgttg ggctagataa cgttgatatt cctgctgcaa ctgctgctgg agtaatggtt
    30601 gttaatgctc ctacatcaaa tattgtcagc gcagcagagc ttgccattgc acttcttctt
    30661 gcatctgctc gccatctatc acctgctcac gccgcactaa agggcggaaa gtgggctcgc
    30721 tctaaataca ccggcgctga gttatttgaa aagactctag gtgttgttgg ctttggaaga
    30781 attggtcaac tagttgctca tcgcatgcag gcatttggca tgaaagtaat tgcatatgac
    30841 ccatatcttc aaccagcaaa agctgcagca cttggggtag atctagtctc acttgatgat
    30901 ttgctagcaa gctctgattt cataacgatt catcttccaa aaacaaaaga gacagctaat
    30961 ttaattggcg atgcagcgct gagcaaagtt aagccaactg ttcgaatcat taatgctgct
    31021 cgcgggggag tattagatga agctgcgctc tataaagcta ttagccaggg gcgagtagca
    31081 ggtgctggac ttgatgtatt tgcaaccgag ccttgcactg attcaccgct ttttactcta
    31141 gatcaagttg ttgcaactcc tcatcttggc gcatcaacag atgaagccca agaaagagca
    31201 ggaattgccg tagcagtatc agttcgcaag gcgctctcag gagagcttgt gccagatgcg
    31261 gtaaatgtta aaggcggcga gattcatgaa gagatccgtc caacgcttcc actagttgaa
    31321 aagatggccc agattgcaac cggccttgaa gcagagcttc cagtaagtat tgaaatcact
    31381 gttaaaggtg aagtctctat ttatgactca tcaattttgg caacttctgc gctaaaggga
    31441 gcgctaattg caattggaac cgaagatgcc acttatgtta actccccaaa tcttgcgcaa
    31501 gaaaaaggta tgagcgcaac agttacaagt gaggctgagt gcgaggatta tcgaagcatg
    31561 atctgccttc gcgctgcttt ctcaaatggc tcaagagttg aagtagatgc aactcttatg
    31621 ggaattcgaa aagtagagaa gataattcgt atcaataagt ttgatattga gctaccacca
    31681 agtgatcatc ttctatttct tgtctatgaa gataagcctg gagttgtcgg agctgttgga
    31741 aatattcttg gcgctgcaaa aattaatatc gccaatatgc aagttgctag agataaggcg
    31801 ggcggtgagg ctctaatggc gctaactgtt gactcggcta ttccaattga gttcacagaa
    31861 aagatcgcta aagaggttgg agctcgggta tctagatcgg tatccctact ggtatgagta
    31921 agaactttaa tcttgcagta atcgcaggcg atggaatcgg acctgaggtt gttaatgagg
    31981 gtttgaaaat tcttgatgca gcgggcaaga agcatggctt tacctatagc aagaaagcct
    32041 ttgaattagg ggctgccttc tggcataaaa ccggtgaagt tctgccagat aaaacgctgg
    32101 aagaactaaa aagctttgat gtaatccttc ttggcgcagt aggtgatcca agtgttccaa
    32161 gtggagtcct tgagcgcggg ttacttctaa aacttcgttt tgcatttgat cactacatca
    32221 acctgcgccc ggcacgccta tatccagggg tagttggtcc gctaaagaca agtgcgccga
    32281 ttgatttcat tgtagttaga gagggaaccg aaggacctta tgttggagct ggcggagttc
    32341 ttggctttgg cacttcaagt gaaatcgcaa cagaggagag cctcaatacc agacgaggcg
    32401 cagagcgagt tataagagat gcatttaata gagctatggc aagacctaga aagaaattaa
    32461 ctttagttca taagaataat gttttaacaa gagcgggaag cctttggact agaacctttg
    32521 aagaagttgc aaaggagttt cctcaagtaa gcactgatta tctccatgtt gatgcagcct
    32581 cgatgttctt tgtaacaaat ccggagcgct ttgatgtagt ggtaactgat aatctctttg
    32641 gagatatttt gactgatatc gctgctgcta tctgcggcgg aattggcctt gctgcctctg
    32701 gcaacatcaa tccaagtggt gagtttcctt caatgtttga accagtacat ggctcagccc
    32761 ctgatatcgc aggaaaagga attgctgatc caactgcaac aattatgtct gttgcgatgc
    32821 tacttaatca cttagggctt agtgatgctg caagagatgt tgaaaaagca gttgaggctg
    32881 acttgcttac aagagcagat aagaagcgaa gcactagtga aataggcgat gacctagcaa
    32941 aggctcttag ctaatgaaaa ttacagtcat cccttcatca aatccaatct caccaactca
    33001 gagagatcag tatttagagg cgcctggatt tggaaaatac ttcacagatc acatggcagt
    33061 tgctgaatgg aatcagtcga gtggttggtc tgatctaaca atttctgcct atgggccgct
    33121 caatttagat cctgctgcaa tggtttttca ttatgggcaa gaaatctttg aaggaatgaa
    33181 ggcttactac caaccagatg gatctatcgc gctctttcgc cctgatgcga atgctcttcg
    33241 ttttgcaaag agcgcagcgc gaatcactct tccagagctt ccagttgaag actttgttag
    33301 cgccgttgaa gctttggtaa agcaagatgc tggctgggtt ccaaagaaag ttggcgagtc
    33361 tctttatttg aggcctttta tgattgcaac tgaagttggt cttggagtaa ggccatctaa
    33421 taaagcttta tttgcagtaa ttgccacccc tgcagctgct tactttaatt ctgctaaagc
    33481 tgtaacagtt tggatctctt tagagtatgt cagagcagta cttggtggaa ctggcgaggc
    33541 aaaatgtggt ggaaattatg ccgcctctct tcttgctcag caggaggcag caaaagaggg
    33601 ttgtgatcaa gttgtctggc ttgatgccaa agagcgattc tgggttgaag agatgggcgg
    33661 aatgaatctc tactttgtta agggtagagg aaaagatgca actatcttca ctccaaaatt
    33721 aactggaacc ttgcttcatg gcatcacaag agattcaatt ttaagcgttg ctaaagattt
    33781 gggatataaa gtcgaagagg gaatgatttc tattgatcaa tggcgagatg gcgttgcaag
    33841 tggagagatt tctgaaatct ttgcctgcgg aacagcagca gttattgcac ctgttggttc
    33901 tgcaaagagt aagtatggaa cctgggtaac cggggatggc aatccagggc cgataactat
    33961 ggaaattcgt aatcacttac tcggtattca acatggcact atcccagata aacatggctg
    34021 gatgaagcga gtcatctagt ggcaatctct gattcatttc atatctatga caccacacta
    34081 agagatggcg cgcagcaaga gggtcttaat ctctcagtcc atgacaaatt aactatcgcc
    34141 agacatcttg atgatcttgg agtgggcttt attgaagggg gatggcctgg agctaatcca
    34201 aaagatactg aattcttcaa attagccgct aaagagttga agttaaagaa tgccactttt
    34261 gtcgcatttg gcgctactag gcgggccaat accaaagcag cagatgacgt acttcttaga
    34321 gcgctggctg aatctggagc gccagttgta actcttgttg caaaatcaca tgatcgccat
    34381 gttgagctag ctttaaaaac agatttacaa gaaaacctag cgatgattaa ggattcaatt
    34441 aagtttttaa ggcaaggcgg ccagagagta tttcttgatg cagaacattt ctttgatggt
    34501 tatcgagcaa atccagcata cgcacttgag gtagttagag ttgcagcaga ggctggagca
    34561 gatgtcattg ctctctgcga taccaatggc gggatgctgc ctgatgaact atcagatgta
    34621 gttgcaaaag taatttcaga tagcgcagct cgcattggaa tccattgcca taatgacaca
    34681 ggatgcgcta tcgctaactc actggctgct gttgcagctg gcgctagcca tgttcaagga
    34741 actttaaatg gctatggcga gagaactgga aatgcagatc taatttcaat cattgccaac
    34801 ctgcagctaa agaagaaaca agaagtactt ccggctggag cgctagagga ggcatttaga
    34861 atcgctcacg ctgttgctga agttaccaat gtctcaccaa gtgccagaca gccatatgtt
    34921 ggtatctctg cctttgcaca taaagccggt cttcatgcat cggcaattaa agttgatcca
    34981 gcgctctatc aacatgaaaa ccctgaaagc gttggtaacg atatgagaat gctcgtttct
    35041 gaaatggcag gaagggcatc aattgaaatt aaatcctctc aacttggttt tgacttaagt
    35101 aaagaaaaag aggttgtcgc tcgcttagtt gagcgagtaa aagaattgga atctggtggt
    35161 tacacatttg aggcagcaga tgccagcttt gagctgttat tacgagaaga gcttgctgga
    35221 aagaaaccct catttttcac tattgagagt tggaaaacta gcgttgatca aggcgcagat
    35281 ggttcagtaa cttcaagagc tgaagtgagt attaaagcaa agggtgagtt aatttcatcc
    35341 tctggtagtg gaaatggtcc agttaacgct atcgatagag cgctacgaaa tggtcttgag
    35401 aagttatatc cagaattagc tgagcttgag ctaactgatt acaaagttcg tatcttggaa
    35461 ggtcgcctag gaactggggc tattactcga gtattagttg agagcagcga tggtcatggc
    35521 gaatggagca caattggagt tcatgaaaat gttattgccg cctctgccat ggcgctagaa
    35581 gatgcgctaa cttatgggct aataagagca ggaaagaaac ctgagtaaag gttttaagta
    35641 aggagagaaa agaatttagc tatgcgcacg gttttaaaaa ggggagtgaa attaactcct
    35701 agcgaatcat cagagtggct aagagcaaga atggaacagt taaggatttc agggcttgaa
    35761 gaacttcact taaaaacagg aatcgataag ggctcaatct ctcgctattt tcgccaagag
    35821 cgcaccccaa aaattgatgt cattgcccct cttgctcaag cacttgaagt ctcgccagag
    35881 actctgctta tcgcacttgg cgcaatagat aagaagcgca gctaatcact ctcttgcagt
    35941 gatgattagc tcatctccgg ttcgattaaa gtctctagca aaagcatcat ctgcccagag
    36001 gcgatgcagg tgagctatct gctcagtatc taatatcccg gatgtaacat ctaaaatctg
    36061 atcaaagtca tcatttattc caatggtgac ccttatttca gttgcagtat ttgtaaggct
    36121 tggctcaagg gcagtatcac ttattactaa gagaattttc aattgctgcc ccttcgccat
    36181 ttagtagcta agtgattcac ttacctctga caaaccaccg aagaagatcc acactttgcg
    36241 catctgaaac agcgtggatt aagtgttgct tagaagcaac gctgtgtcta tcgttcttag
    36301 aggtgattcc tagataacct agggtttatg gcgctagata gtgagttggc aaaggcttat
    36361 cgcagccatc taatctctga gcgaaatctc tcagagaact caattagggc ataccttgct
    36421 gatttagagt cgctcctact tcatataaat aaattgggag tctcagagtt tgcccagcta
    36481 gagctcaacc acattcgctc ttggcttgca aacctttcaa ccaagggagc agcgagatca
    36541 tcaattaccc gccgagtagt ttcaattcga gcctttacct attggggggc aagaagtggc
    36601 tggctttcaa gagatattgg taaagattta attgctccaa agcctgagag aagcctgcct
    36661 gatgttttag atatcgaaag tgcagcgcta acaataaaag cacttgaggt aagagcgcag
    36721 gaagaggaaa gtgctagctc gcttagagat ctagcgttag ttgaagtctt atatggctca
    36781 ggcattcgta tctcagagct tgttggtcta gatcttggtg atattgatag gcagagatca
    36841 acaattaaag tgatgggtaa aggaagtaaa gagagagttg ttcctcttgg ccaaccagcg
    36901 ctaactgctg ttgataattg gataaataag ggaagagcag agctggtaag tgaagcaagt
    36961 ggtagcgcac tatttattgg ttctcgtggc aagagaattg atcaaagagt tgctagaagc
    37021 gttgtttatc aagcgatgga ggcaattggt agcgataaga aattagggcc acatactctt
    37081 agacatagcg cagcaactca tttacttgaa ggtggcgctg atttaagaac tgttcaagag
    37141 attctcggcc actcatctct tgctactact cagatatata cccatgt
//
LOCUS       DAOROD010000011        32743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_39_length_32743_cov_28.228953, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000011 DAOROD010000000
VERSION     DAOROD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 32743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32743
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39_length_32743_cov_28.228953"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..396)
                     /locus_tag="PLW53_03565"
     CDS             complement(196..396)
                     /locus_tag="PLW53_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36532.1"
                     /translation="MAAKRRKKAAAKAAPKRRRKKAAAKAAPKRRRRKKAAAKKAAPK
                     RRRKKAAAKAAPKRRRRKKAAK"
     gene            complement(661..1929)
                     /locus_tag="PLW53_03570"
     CDS             complement(661..1929)
                     /locus_tag="PLW53_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36533.1"
                     /translation="MRKYAIDLSPLKKYRDFKLLFTAGLFSYFGSMITFVALPFQVKE
                     LTGSFWAVGLIGVVEIIPLIVFGLYGGVLADYLDRKKMIWFTEFGTLIATSILLINSL
                     QEKPSVILIFAIAAIFAALSGLKRPSQDAILPRLVNHDDLPSASALMSLRWQFGGIVG
                     PSVGGIIIASYGAGAAYFIDCLTFVISLALIWQVRSVPPLDKSTPPSMAGLMEGINYA
                     FKRKDLLGTYVVDLAAMFLAMPMALFPFWADEIGAPYALGFFYSSITFGAVLVTLLSG
                     WMRNYPHHGRAVIFGALGWGVAIVAAGSSNSLVLVITCLVIAGAFDQVSALFRGFIWN
                     QSIPDELRGRLAGIEMLSYLLGPLGGQARAGGMAAMSSLKTSIVGGGLLCIGFVSLIA
                     AVMPKFRSYDVRTNEYALKEAKIRKNRENI"
     gene            complement(1938..2732)
                     /gene="panB"
                     /locus_tag="PLW53_03575"
     CDS             complement(1938..2732)
                     /gene="panB"
                     /locus_tag="PLW53_03575"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRD36534.1"
                     /translation="MARYTVLDLAAWKKTGKPFAMLTAYDSTMASVFDQAGVPILLVG
                     DSAGNNFLGHENTIPVTLDELISLSRAVVKGSEKALVIADLPFGSYEASPQLALESSI
                     RLLKEAGVAGVKLEGGVRVCEQVKALVSSGIPVMGHLGMTPQSVNAFGGFKVQGRDSK
                     ADEIIKDALALQEAGAFALVLELIPAKLAKEITETLSIPTIGIGAGVNCDGQVLVWTD
                     LMGLTPKPPRFSRAYLNLRQDMVEAVGKWLLDVESGSFPSAKESFD"
     gene            2804..4489
                     /locus_tag="PLW53_03580"
     CDS             2804..4489
                     /locus_tag="PLW53_03580"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRD36535.1"
                     /translation="MPTAKSAPLRIAMAQINPVLGDLEGNSQLILGAVKEALVSGADV
                     VLFSEMVLTGYPVEDLALRPAFQSASRRRVNELAEQIEKIGGGELLIIIGYLDHNEKP
                     ENALAAIHRGKVVGKYVKHHLPNYGVFDEFRNFGQGDKSLVIRFGSIDIALAICEDIW
                     QEGGPVANLSKANPGILLVANGSPFERDKDGVRLDLARKRVKQLGCPLVYLNMVGAQD
                     DLVFDGGSFALDSNGEVIAKSEQFESLLNIVDIECEIESGSPDLVLSGAIKKDKGAAR
                     TLIAPSLSDEEEIWKALVVGLKDYVSKNGFKSVALGLSGGIDSALTATLAVDALGKES
                     VYGLLMPSKYSSTHSISDAKELAENLGIGYEIIEIGSVVSSFQSAIELSGLAEENIQA
                     RVRGSLLMGLSNQRGHLILATGNKSELAVGYSTLYGDAVGGFAPIKDIFKVDIWRLAR
                     WRNSQAGGKGLIPVNSIEKAPSAELSPGQRDSDSLPDYELLDQVLKLYIEKNIDATKI
                     IASGFDKALVDRVVAMVDKAEYKRRQYPPGPKVSPIAFGKDRRLPMTSRWKES"
     gene            4724..5653
                     /gene="cysK"
                     /locus_tag="PLW53_03585"
     CDS             4724..5653
                     /gene="cysK"
                     /locus_tag="PLW53_03585"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRD36536.1"
                     /translation="MKVYENITQVYGNTPLVRLNRITDGAEATVYAKLEFYNPTSTVK
                     DRIGIAMVDAAEKSGALKPGGTIVEATSGNTGIALAMVGAARGYKTILTMPDSMSKER
                     RTLLRAFGAELVLTPAAEGMKGAVAKAEELGANGAVMVRQFENEANPEVHRRTTAEEI
                     WRDTDGLVDAVVAGIGTGGTITGIGQVLKSRKPEVKIFAVEPAASPILNGGKPGGHPI
                     QGIGPNFIPPILDRSVYDEVLDVSAEDAVKYARRAAAEEGILAGISSGGALWAAAQVA
                     RRKEFAGKNIVVIIPSFGERYLSTILYQDLMDS"
     gene            5658..6164
                     /gene="cysE"
                     /locus_tag="PLW53_03590"
     CDS             5658..6164
                     /gene="cysE"
                     /locus_tag="PLW53_03590"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRD36537.1"
                     /translation="MFKVISQDLETALLKDPAARNKLEVFLTYPGVHALWGYRICHWL
                     WNRKLKLISRIYSNWIRSITGIEIHPAAKIGKRFFIDHGMGVVIGETTVIGDDVMIYH
                     DVTLGARTFENGKRHPTIGNRVVIGAGARVLGDIKIGDGVRISANSVVVKDVSPQSDI
                     DASEQFAI"
     gene            6222..7538
                     /locus_tag="PLW53_03595"
     CDS             6222..7538
                     /locus_tag="PLW53_03595"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRD36538.1"
                     /translation="MAKEDIEIAKQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIA
                     PPELENAFAEGIGFDGSAIEGFARGSESDMLLKPDPATFSILPWRTEKPGAARIFCDI
                     ALPDSAPSPADSRNVLKRVLEKAAKMGYTCYTHPEIEFFLFKDAPALGVKPTPVDAGG
                     YFDQTPIAIGQDFRRQAITLLEAMGVSVEFSHHEVAPGQQEIDLRYADALSTADNIMT
                     FRHIIKEVALDQGCHASFMPKPFTEHPGSGMHTHFSLFEGEKNAFYKDGSTSGELSNV
                     GKSFIAGILKHAPEFIAVTNQWVNSYKRLHGGGEAPAKISWGAQNRDAMIRIPRYKPN
                     KENSTRIEFRAPDSACNPYLAFAVTIAAGLKGVESGYELGIDESPASLPADLNEAILA
                     MQESELVRQCLGEHLFDYFLRNKRAEWLEYSKQVTAFELDRYLPVL"
     gene            7714..9429
                     /gene="leuA"
                     /locus_tag="PLW53_03600"
     CDS             7714..9429
                     /gene="leuA"
                     /locus_tag="PLW53_03600"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRD36539.1"
                     /translation="MNFPKQEASKMAKHRYSSFIPIKLDDRTWPSKTMKEAPKWCSVD
                     LRDGNQALIDPMDVPRKLAMFSLLIKMGYKEIEVGFPSASQTDFDFVRKIIEDGLIPD
                     DVTIQVLTQARDALIRRTYESLVGAKSAVVHLYNSTSTLQRRVVFGQDKEGIKKIAVD
                     GAKLCKELVSTLPDTKISFEYSPESYTGTELEFALEVCNAVNDIWQPTPTHKSIINLP
                     ATVEMATPNVYADSIEWMGRNLKYRDSIVLSLHPHNDRGTGVAAAELGYLAGADRIEG
                     TLFGNGERTGNVCLVTLGLNLLSHGIDPMIDFSDIPEIRRTVEYVNQLRVPERHPYGG
                     DLVFTAFSGSHQDAIKKGFEHLERDAKAAGKKVDDFTWAVPYLPIDPKDIGRSYEAVI
                     RVNSQSGKGGVAYVMKSEHQLDLPRRLQIEFSHVIQSHTDDQGGEVFPDQMWKIFVDE
                     YLPSEDNKWGRFRFTSLNQSSSAGKDVELSVDLLDNQKQITLSGSGNGPISAFCNVMQ
                     SYGVDIQVADYYEHAMSAGGDASAAAYLECTVNGGTYWGVGIDPSTTTASLKAVISAV
                     NRALR"
     gene            9452..10189
                     /gene="recO"
                     /locus_tag="PLW53_03605"
     CDS             9452..10189
                     /gene="recO"
                     /locus_tag="PLW53_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRD36540.1"
                     /translation="MALYRDEAVVIRTQKLGEADRIVTLFSRQRGRIRAVAKGVRRTK
                     SKFGARLEPASYVDIQLYTGKTFDVVTQVEAIENYGDAISGDYQRWTIASAILETAER
                     FTNNEGEPALQQFLLLTGALKSLAHQSHDPSLILDAYLLRSLSIAGYAPSMTICSRCE
                     APGPHKYFSLVGGGSVCLDCRPSASATPALETLQLMSDLVSGDWKSAEQSELKNRREA
                     SGLIAAYLQWHLERNLRSLPMVERVIL"
     gene            10186..10908
                     /locus_tag="PLW53_03610"
     CDS             10186..10908
                     /locus_tag="PLW53_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRD36541.1"
                     /translation="MKVPKIAKEKIPHHVALVMDGNGRWAKARGLARTKGHEAGEAAL
                     FDVVQGAIEIGVKELSAFAFSTENWRRSPDEVKFLMGFNRDVIRRRRDQMNEMGVRIR
                     WVGAPKRLWKSVIEELEIAQELTKKNKVLTLNMCINYGGRAEIASAAQEIARDLAKKK
                     IKADQINERLFAKYLDEPGMSDVDLFLRSSGEQRTSNFLIWQSAYAELVFMDVLWPDV
                     TRQTLWQAIEIYSKRDRRFGKA"
     gene            10910..>11478
                     /locus_tag="PLW53_03615"
     CDS             10910..>11478
                     /locus_tag="PLW53_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD36542.1"
                     /translation="MSKSNVQFWFDPICPWAFMTSRWLLEVEKIRDISITWNLFSLAH
                     LNKDKDLAPEYKARLIRSWQATRVIAAAQEKNGKEITLPLYTAISSRIHLKKMEVGKE
                     LFTQALKEVGADENLASAESDSKFDNAIIASHEAGIALVGSDVGTPIIAVEGTAFFGP
                     VISPAPKGEEAGKLWDGVLLAASYPGFFE"
     assembly_gap    11479..11578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11579..12051)
                     /locus_tag="PLW53_03620"
     CDS             complement(<11579..12051)
                     /locus_tag="PLW53_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36543.1"
                     /translation="MNLPILLAAATALATAAGGLLALKAKDRLHLVLGLAAGLLLGLV
                     AFDLLPEVFEMSSATLLGAPAVSVALVGGFLLLHLYEKFFGTHEPAESDFSDDHKHRV
                     NVAGGLGAFAMAGHVFLDGLALGVAFTIDEKLGIAVFVALLVHAFSDGLNTVSLL"
     gene            12206..13582
                     /locus_tag="PLW53_03625"
     CDS             12206..13582
                     /locus_tag="PLW53_03625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRD36544.1"
                     /translation="MADRLEVIVSLAKRRGFVFPSSEIYGGLRASWDYGPLGVELKNN
                     VKRQWWKSMVTGREDVVGLDSCVILAKEVWEASGHVETFSDPLTECTECHKRYRADHL
                     EEAYEAKHGKKPTSLSEINCPHCGNKGKFTVPKQFSGLLKTFLGPVEDESGLAYLRPE
                     TAQGIFINFDNVATTARKKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVAP
                     GSDEEWHQRWIDTRLAWYVDLGINPQRLRLFEHPKEKLSHYSKRTVDIEYNFEFAGTQ
                     WGELEGIANRTNFDLKAHSEKSGKDLSFFDQEKNERWFPYVIEPAAGVDRCALTFLMD
                     AYSEDEAPNSKGEMEKRVVLRLDYRLAPVKVAVLPLSRNADLSPKARDLAAALRKNWS
                     VDFDDAGAIGRRYRRQDEIGTPYAITVDFDTLNDNAVTIRERDSMKQERISIDQVEAW
                     LAPRLLGC"
     gene            13552..14724
                     /gene="dusB"
                     /locus_tag="PLW53_03630"
     CDS             13552..14724
                     /gene="dusB"
                     /locus_tag="PLW53_03630"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRD36545.1"
                     /translation="MVSPTATGLLDLKPLHLPIRGGGLDLTAPVVLAPMAGITNAAFR
                     LLCREQGAALFVSEMVTARALIEGNDETLRMITPGVGEHPRSIQLYATTAEDLSRAVE
                     ILGSRDLADHIDLNFGCPVPKVTRKGGGSALPYKRRLFASLVEAAVKGAGKYQIPVTV
                     KMRVGIDSQYETYLDAGKIAADLGVAWVALHARTAAQLYEGKSDWSKIKNLVEHLAPT
                     GIPVLGNGDIWSGSDAIEMVKTTGCAGVVVGRGCLGRPWLFADLVAAFNGQELRALPT
                     LFQVREIIYRHAELLTQYFSSEDRACRDLRKHMAWYLKGFRVERELRAKFGMVSSLLE
                     LRQLLDQLVDQPYPVAIGDAPRGRTSHGRAVSLPDGWLNDPEELVTVDGQDSGSGG"
     gene            14724..15347
                     /locus_tag="PLW53_03635"
     CDS             14724..15347
                     /locus_tag="PLW53_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRD36546.1"
                     /translation="MFLFKFAWKFISFLLLVALVIPLWAIGKTWYTAQNETIRKADAI
                     VVMGAAQLDGRPGEVLEARLKESLRIYEKGFATEIYTLGAGAPGDRFTEAAAARSYLI
                     SKGVRAKSITAVAKGRDTYRSIEALSKAEGIKRIKEIIIVTDPYHCLRSMTMANDFGF
                     NATCSPTRVGIASLEEAGFRYLLRESGAYLAYLTLGRRGIHISDHVA"
     gene            15355..16596
                     /locus_tag="PLW53_03640"
     CDS             15355..16596
                     /locus_tag="PLW53_03640"
                     /inference="COORDINATES: protein motif:HMM:NF002829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRD36547.1"
                     /translation="MALHPANEHLGYSELDRERFLDEPAKRKGRTEFARDRARIIHSF
                     ALRRLAAKTQVAVPWASDFPRTRLSHSLECAQVGREIGAALGADPDLLESACLAHDLG
                     HPPFGHNGEKALAKIAKDIGGFEGNAQSFRLLTRIEAKTVDQNGLSVGLNLTRATLDA
                     ATKYPWSSKENPEKFGVYQDDLEIFNWVRQESLESRTCMEAQIMDWSDDVAYSVHDLE
                     DGLVTGQIDISKLNDDLEHLYQTAIRDYLPDLSEQEAASALNSLHNLSCWPKEFDRSH
                     RHLAQLKDLTSQLIGRFALFAERATRERYGEGNLTRYQANLIVPREVRIEVALLKSMA
                     GYYIIGAEVSKERYARQEEVIIELVEAVSRLAPASLESFFLQQWQVADSAEAKMRVVI
                     DQVASLTDMGAYALHEKLIGA"
     gene            16607..18442
                     /gene="dnaG"
                     /locus_tag="PLW53_03645"
     CDS             16607..18442
                     /gene="dnaG"
                     /locus_tag="PLW53_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRD36548.1"
                     /translation="MSGRIRDTDVSYVRDHSPIDEVVGDYVQLKSAGGGQKKGLCPFH
                     DEKSPSFHVTPARGYFHCFGCQEGGDVIAFIMKIDHLSFTETIERLASRIGYQLTYEQ
                     SSGGSKAPTVQRSRLLEANSQASLFYQEQLQLPTASHARELLIKRGFDKTASQSFEIG
                     YAPDQWDALSKFLLGKGFSAEELLLAGLTKEGTKGAIDRFRNRLIWPIRDISGDLVGF
                     GARKLASDEEDTGPKYLNTPETSIYKKSQVLYGLDKAKKEIAKKRQVVIVEGYTDVMA
                     AHLAGIPTAVATCGTAFGDEHIRILRRLLMDDDAFRGEVIFTFDGDAAGQKAALRAFG
                     DDQKFVTQTFVAVEPSGLDPSDLRQEMGDAALRDLIARRVPLFEFAIKSSIKQFDLGN
                     ADGRVQALNAAAPLIGKIRDASLRPEYARSLAGWLGMEVEVVMAAVKKNTSTKPANND
                     AVVAGEQWRPDPSEPRLILEREVLKVRLQMPALVRNWPELEKNAFSHPAYIKLREFID
                     TQEDLMAVNLEATDSDELKSFITELMVEPIRTDGEISDRYVSSITARLNEVALARSIA
                     EVKSSLQRLNPLESESEYNQMFSRLVEMEAKRRAQRELALGESLT"
     gene            18460..18532
                     /locus_tag="PLW53_03650"
     tRNA            18460..18532
                     /locus_tag="PLW53_03650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18493..18495,aa:Asn,seq:gtt)
     gene            18555..18794
                     /locus_tag="PLW53_03655"
     CDS             18555..18794
                     /locus_tag="PLW53_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRD36549.1"
                     /translation="MAMVRIRVALPDRPGSLGAVASAIGFAGGDIRGLVVLSSEGGRG
                     IDDITVAFPGSDPQDLVNVLSAIGGVEVISVTPVV"
     gene            complement(18791..19918)
                     /gene="galK"
                     /locus_tag="PLW53_03660"
     CDS             complement(18791..19918)
                     /gene="galK"
                     /locus_tag="PLW53_03660"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRD36550.1"
                     /translation="MEITELFTKGFGYPPAVVASAPGRVNLIGEHVDYSGGFVLPFAI
                     DAVTMCAVSKRDDRKIRIISAQKKNEMIESNLEAIAAKDGSSWSRYIYGVIWAMEINS
                     GLDIYIDGKVPLGAGLSSSAALECSLATALNHLFHLEKTLPELARLTQRAENEYVRVP
                     CGIMDQSISLMARAGYGLLLDCQDLSTRHIKIDFASNSLRLLIIDTQAHHALTDGGYA
                     KRRESCEEVAKIFSINSLRQLTMDSLLAHKTKLSDIQFKRARHGVSEMARVLDAAKAL
                     ETGDFKALGQLLNQSHNSLRDDYEVSCPELDLAVDTALDSGALGARMVGGGFGGSAIA
                     LINESDAGAISLAIEKAFAQSGFKAPRFFDSLPSDGAKVIS"
     gene            complement(19925..21034)
                     /gene="galT"
                     /locus_tag="PLW53_03665"
     CDS             complement(19925..21034)
                     /gene="galT"
                     /locus_tag="PLW53_03665"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRD36551.1"
                     /translation="MSSKKLPAGVIRSDATLMDGRTIRYYDTKGQSRTAVDQRSSEEQ
                     PGIGEMRLDPLNNEWVVIAAHRQERVFLPPKELNPLAPSKPGSLTEIPESDYEVVVFD
                     NRSPSLAAPRSSFEAIDNPDFSSPAIDSAGKCEVICFTSDYEASFKDLSHRQIRTVLE
                     AWRDRTAELSELSYIEYIAAFENRGEEVGVTLNHPHGQIYAYSYLPPKAERALAVATA
                     HKKKTGNILLEDILARELSDKVRIVAQNEEWVAYVPYASRYPFEIHLAPRRSVPDFLA
                     LDEAASDAFAPLAKEVLLRLDGVFGVKMPYIASWHQAPVRVGRDVTRLHWQIASVRRA
                     PGKLKYLAGSESAFGAFMMDLKPEQSAAQLRDVAL"
     gene            21056..23176
                     /locus_tag="PLW53_03670"
     CDS             21056..23176
                     /locus_tag="PLW53_03670"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRD36552.1"
                     /translation="MSSPIPSSKAGARAQLSNGGVDLLLEVIDGAPVIRYWGASFKGE
                     ASELLFDRSIANSDFDAVANPGMMREHSRGHLGYPTIKGHRAGLDWSTKFSVKELKSD
                     KSSFQIQLEDKDAKLTLDIGASLDQFGVLKLWQELTNLGDTYFLDELNYWMPLSDSAT
                     QSLDFVGRWSNERNPQRRDIAIGRYVRDSHEGRPGHNYTIGMIALEKETNFASGQSWA
                     LSLAWSGDSQYCIEKNYEGSASISAGEVLLPGEVILEKGQSYRAPDLFALYSNAGLDG
                     LSSNMHKHLRARSVHPKNPRPMTLNLWEAIYFDHNEEKIKKIVDTAAEAGFERVVLDD
                     GWFGSRRSDLSGLGDWQVSKEVWPSGLGSLANYIRDKGMEFGLWFEGEMVNPDSDLYR
                     SHPDWILKVGDRVGPTWRHELVLNLDNQDAYNYVLDSVDKILTQYQITYIKWDHNRVL
                     IDAGSNNRAAVRKQTQGLYRLFAELKKRHKNLEIESCASGGARIDLGIIDLVDRFWTS
                     DNNDALERARIQRWSAQFIPPELLGTHIGADPGHQTGRSISFSFRALTALFGHAGVER
                     DITQLSSSELAELKSWIALYKAKRKLLHSGDVIRIDYPKDSHYLNGVISSDKKSALFS
                     FACLETIPESHPPRMRIRGLDSDRRYSVKVLSGGQKSNFMAIEPPIWITTGLELTGSE
                     LENIGLSAPILRPASALLIEITAL"
     gene            complement(23173..24672)
                     /gene="glpK"
                     /locus_tag="PLW53_03675"
     CDS             complement(23173..24672)
                     /gene="glpK"
                     /locus_tag="PLW53_03675"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRD36553.1"
                     /translation="MGYIGALDQGTSSTRFIIFNQTGQIVGQEQLEHSQILPKAGWVE
                     HDGAEIWRACISVITGALKRAGIQGSDLNSIGITNQRETTLIWDKSTGLPLHNAIVWQ
                     DTRTAEFLNALSDQVKEKIVYKTGLAIAPYFSASKINWLIKNSPEVALAIKDGRALAG
                     TIDSWLLWNLTGGVNGGVHYTDVTNASRTLLMNLESLDWDSELLEIFEIPRQILAEIK
                     SSSQVYGHSLSNGPLASSVPISGILGDQQAAMVGQTCFELGESKTTYGTGNFALLNTG
                     TSIVRSKHGLLTTLCYKFGDDKARYALEGSVAVTGSAIQWLRDQLGIITSAPQVEELA
                     KSVSDNGGVYFVPAFSGLFAPYWRSDARGVIVGLSRAATKAHLARAALEAICYQTRDV
                     LDAMSADSKVALTEIRVDGGITANELCMQMQADIMGVEVIRPVIIETTALGAAYAAGL
                     ATGFYKDTSELKAKWREDKRFKPDKASTLATTGFKNWKKAIEKTLNWLD"
     gene            24691..26367
                     /gene="glpD"
                     /locus_tag="PLW53_03680"
     CDS             24691..26367
                     /gene="glpD"
                     /locus_tag="PLW53_03680"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRD36554.1"
                     /translation="MSRSNSSLNVASRSAAITALGESEFDILVIGGGINGVGIALDAA
                     SRGLSVALVESGDFASGTSSKSSKLIHGGLRYLEQYDFRLVREALYERELMVTTLSPH
                     LVKPVSFLYPLHEKLIERTYVGAGMALYDALRGFKRALPWHKHLSQKKVSEIAPSLRL
                     DVITGGFQYFDAQVDDARHTMSIARTAAKYGAVITTRTRCEEVIKNGKRVVGAKIRDL
                     LSDEVIEVRAKATIMASGVWSDQLHEKFGLKAGYKVRMSKGSHIVVPKSAINSQSGVI
                     IKTDVSVLFIIPWGEQWIVGTTDTDYQESKEEPLASSDDVSYIINQANRVLEPKLRRD
                     QVVGVFAGLRPLVSTDPDSPTTTLSREHVVDSPTPGFVSIAGGKYTTYRVMSEDAVDE
                     AVNHLRRIVPDSTTENLAIIGAEGYSVLINKIPKLAKEYGLSEETIRHLLDRYGSLFD
                     EVLAPAKEDTSLLEPLIEGLLYIKAEALYAITHEAALSIDDLLSRRTRIAFEASDSGL
                     SITSYLGELLGNFAGLGKKEITKSITEYEQIIMRQKKNLEKATQMESKVG"
     gene            complement(26364..27983)
                     /locus_tag="PLW53_03685"
     CDS             complement(26364..27983)
                     /locus_tag="PLW53_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRD36555.1"
                     /translation="MISPRRISLLLLIWALLANISSPISQAATTSSFNLTQTPAFPDY
                     KVTFHGVIKPKVKNAVVKIDVKIGNSWSDTRLRTKSTPSGAWKIQARATALAANATYR
                     AKVIIGGKTLITKTKRIVIEQNPAISTPDELISLSGPGGRIHGTDISRWQHPQDKPID
                     FVKMYNAGIRFVMIKASDTRDDADALALKYLLMDRDAAQAAGIYTGYYHYTILPNTTN
                     KESVILDAKAQAQKALWRLSSLGGYTEKDLPYALDLEYNCVQVSNGSCVKYAPKPLVT
                     LWAETWLETMYEKTGRKPFLYSYPTFLEQAMVRSEKLRQYPLWKAQYGINPADPIAEP
                     GRKEFGCFVHSWSTANCSSLWQIWQYSSCGIGEKYGVPSPRVDLNVFRGDSASFLQLT
                     RGIWTPAVGDYLPIKEPTTLLITSVNASSTDKPVKIKVEVKRPSGEPVVTGTVAFRRS
                     PVEGGGPVVKVEQSPVRDRAGIWTLTLRNLPAGLTQGYVAFVDQTGTHLENRQDLSIN
                     LIQGPEVVVTPTPKPTKPVVPFDSCKNQIKN"
     gene            complement(27980..28240)
                     /locus_tag="PLW53_03690"
     CDS             complement(27980..28240)
                     /locus_tag="PLW53_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36556.1"
                     /translation="MELAEIKSRWNDVLDLLERENRVAWLAFFDARLVSFNNNQLKLS
                     FIDAEKLSGAHNYAQVRKDSHRHALEAAIEEIFGVSIVIITE"
     gene            complement(28250..28495)
                     /locus_tag="PLW53_03695"
     CDS             complement(28250..28495)
                     /locus_tag="PLW53_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36557.1"
                     /translation="MSPLSYLRYLAYALFALGLINLRYQTGASDLVLKTVLISGTGVI
                     IFALTYLNFGKAFLAKSFGRTITWIIGIAAIIFAIIN"
     gene            complement(28492..29625)
                     /locus_tag="PLW53_03700"
     CDS             complement(28492..29625)
                     /locus_tag="PLW53_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36558.1"
                     /translation="MKNKSGRLNLDALTKEFGNDKEKVVAVRELTLTINPGEFVTLLG
                     PSGCGKTTALRMIAGFETPTSGKVLLDGEDLSSLTPDKRPMGMVFQSYALFPHMSVFD
                     NVAYGLKIKKMSAAAIKEAVGEVLNSMSLSHLANRAPNQLSGGQQQRVALARAMVVRP
                     KVLLFDEPLSNLDAKLRAQMRIEIRSIQQKLGITSIFVTHDQDEAMSLSDRIVVMRNA
                     VVEQVGTPDEVYRRPATVFVADFIGRSNFLAVETFSKAAANKAKIEVSGKKMVVAAHS
                     KALSSSKPVLLVRPESILVTAAKSRSSKPGKGEGLVENVVFYGNHVEYTLDTDEGRII
                     SVVSDPIYDEIVPVGKIAKYSFDPSRAWLLPSQSSQDVTESRK"
     gene            complement(29635..31335)
                     /locus_tag="PLW53_03705"
     CDS             complement(29635..31335)
                     /locus_tag="PLW53_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRD36559.1"
                     /translation="MSVKASGAKYDIFTKIALVLVIFIIGFLIILPLINILIYSTQPS
                     GSAIITGSFSDVVTRNIIWNTVKLGLTVAALGTVLGFIMAYTQARVEFRGKKILHIIN
                     LIPIISPPFAFATAIIVLFGRSGMITRGVFDWRPTLYGFPGLVLVLTLSFFPIAYMNL
                     LGMLRSLDPALDEAGSSLGANKARIFRTVTLPLLIPGFAGSFLLLFIESIADLANPII
                     IGGNYTVLASRAYMAINGDYDISLAAGYSSLLLLPALLVFLVQRYWAQKKSVVSVTGK
                     PSGRPTLVTSRGAKFFLLSVTGLLTTLIVMVYATVIFGAFVKIIGVNNEFTLDNFRYL
                     LGGFANGAIKTTTMLALIATPLAGVFGMLIAWLVIRKVKRGSEALDFLGMLGIAVPGT
                     VIGIGYAITYNDPVKVAGVTVFPQVAGGAALLGGSIAIVMVYIIRSSPAGQRSGISML
                     QQIDPSIEEASASLGAPGGKTFRLITLPLISGAFLSGLMYAFAHSMTTLSPIIFLVTP
                     DTSILTQKILAEADQGRYGNVFALCCILILIILVIGSLINLLVRRSQVSIERPMVTGR
                     "
     gene            complement(31422..32576)
                     /locus_tag="PLW53_03710"
     CDS             complement(31422..32576)
                     /locus_tag="PLW53_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD36560.1"
                     /translation="MKKSRIIAFAALASLTLSLLSAIAPAQAARDLKGSKCAQEGLVR
                     TIQGVTYTCERVSGSLQYAQGRKLSGTLNVLCTPQEAWCVAMTQAFQAKTGITTKFIR
                     LSSGEALTRLIASKNNPEFDVWTGGPNDSHISGRIQGVLDTYKSPTRNMLKKQFQDSD
                     GYWTGIYMGALGFCSNTNELKRLGLRAPTSWNDLLDPKYKGNIMMAHPGTSGTAYTAL
                     WSQVLRLGSQDAAIAYMKELNKNILSYTRSGAGPTGPLGRGEVATGLVFSHDCTAAKL
                     RGNPVVVSFPKEGTGFEIGGIALVKGAKNAEAAKAYIDFSLSAEAQNLGPAKAESFQI
                     LTNPNGKYDPRMVNLKKVTLLDYQAEAAGVAAVALKARFDKEVALTSSAK"
BASE COUNT         9233 a         6849 c         7712 g         8849 t
ORIGIN      
        1 aggttaagcc caggcccaat acttgccagg tggtcacgat aaatagggtt ccaaggatcc
       61 agcgccagag gcgggggcgc ataataggac tatccatttg agtattcttt caaagtcttg
      121 caggaggagt gcttaggcaa agagaaaagc ccggcgcata tgcagccggg ctttttccgt
      181 aaatctaaag cgtgcttact tagcagcttt cttacgacga cgacgctttg gagcagcctt
      241 tgcagcagct ttcttacgac gacgctttgg agctgccttc tttgcagcag ctttcttacg
      301 acgacgacgc tttggggcag cctttgcagc agccttctta cgacgacgct ttggtgctgc
      361 cttagcagca gctttcttac gacgctttgc agccacgtag tgttcctttc agaatggttc
      421 gatccgtcac cgaacctgtt gcaggacaaa tcgtggcact tattggccaa agtttccact
      481 atttggggaa aatatattgg gtgcgtgtcg gattactttt tctaaaaaaa gtgtgtatag
      541 cgcgattttt atccctcttt ttacgcactt tttacgcgta aatatcgcga ttttcgcaaa
      601 actgaagaaa aaacggcttt ttaaaaccta gttactaaag ggtagggggt taaaaatatt
      661 ttaaatattt tccctatttt tgcgaatttt tgcctctttg agggcatatt cattggtccg
      721 aacatcatag ctacgaaact ttggcatcac tgctgcgatc agagaaacaa agccaataca
      781 gagtaatccg ccgccaacaa tggaagtttt caaggaactc atcgctgcca tgccccccgc
      841 cctggcctgt ccaccaagtg ggccgagaag ataggagagc atctcaattc ctgcaagacg
      901 cccccttagc tcatcaggaa ttgattgatt ccagataaat ccccgaaata aagcgctaac
      961 ttgatcaaag gcgccggcga taactaggca ggtaatcacc agaacaagtg agttactact
     1021 tcctgccgca acgattgcaa ccccccaacc aagggctccg aaaattacag ctcttccatg
     1081 atgggggtaa tttctcatcc agccacttag aagagttaca aggactgcgc caaaggtaat
     1141 tgaagaatag aaaaacccta gggcataagg ggcgccaatt tcatcggccc agaaaggaaa
     1201 aagggccatc ggcattgcaa gaaacatcgc tgctaaatca acaacatagg ttccaagcag
     1261 atcctttctc ttaaaggcgt agttaattcc ctccattaaa cctgccatag atggtggggt
     1321 ggacttatca agtgggggaa cacttctaac ctgccaaatg agcgcaagtg aaattacaaa
     1381 ggttaggcaa tcaataaagt aagcagcacc agccccataa cttgcaatga ttattccgcc
     1441 aactgatggc ccaacgattc cgccaaattg ccatctaaga ctcattagcg cgctggcact
     1501 tggcagatca tcatggttta caagccttgg caaaatcgca tcctggctag ggcgttttag
     1561 cccacttaaa gcagcgaaaa ttgctgcaat agcaaagatc aagataacgc taggtttttc
     1621 ttggagggaa ttaattagaa gaattgaggt agcaataaga gtgccaaatt cagtaaacca
     1681 gatcatcttt tttcgatcta ggtaatcagc taggaccccg ccataaaggc caaagacaat
     1741 aagggggatt atctccacaa caccaattag ccccactgcc caaaatgagc cagttagctc
     1801 tttaacttgg aaaggcaggg cgacgaaggt gatcatcgag ccgaagtagg aaaaaagccc
     1861 tgcggtaaag agcaacttaa agtcgcgata cttctttaag gggcttaaat caatggcgta
     1921 tttgcgcacg ccttatctta gtcaaagctc tctttagcag aggggaagga acctgactca
     1981 acatctagga gccatttgcc aactgcttca accatatctt gccgcagatt tagataggcc
     2041 cttgaaaaac gtggcggctt tggagttagc cccattagat ctgtccaaac tagaacttgg
     2101 ccatcgcagt taaccccagc gccaatacca atagttggaa ttgatagcgt ctctgtaatc
     2161 tctttggcca atttggcagg aatcaactca agcacaagtg caaaggcccc tgcttcttga
     2221 agagcaagag catctttgat gatctcatct gccttgctat ctcttccttg aactttaaaa
     2281 ccgccaaagg cattaacaga ttgcggggtc attccaagat ggcccataac tggaattcca
     2341 ctactaacta gagctttaac ttgctcgcag actctaactc cgccttcaag ttttacccca
     2401 gcaactcctg cctctttcaa taatctgatg ctgctctcaa gagctaattg aggggatgct
     2461 tcataagagc caaatggaag atcggcaatt actagcgctt tctctgagcc tttaactaca
     2521 gctctagata gtgagattaa ttcatctaag gtaactggga tggtgttctc atggcctaag
     2581 aagttattgc ctgcgctatc tccaacaagc aggattggca ccccggcttg atcaaagact
     2641 gaggccatcg ttgagtcata ggccgtgagc atggcaaagg gcttacctgt tttcttccag
     2701 gcagccagat ccaaaacggt atagcgggcc atttactttc cttcctcgag gccaagtgcg
     2761 gtccccggga ttacaaagtg gagtctatcg gtactcttga actatgccta cagccaagag
     2821 cgctccgcta agaattgcta tggcccagat caatcctgtc ttaggtgatt tagagggcaa
     2881 tagccaatta attcttggcg cagttaagga ggcgctagtc agtggggctg atgtagttct
     2941 cttctccgag atggttttaa ctggctatcc agttgaggac cttgccctaa ggcctgcatt
     3001 tcaaagcgct tcgagaaggc gagttaatga actagcagag cagatagaaa aaattggggg
     3061 tggagagctc ttaataatta ttggatatct agaccataat gaaaagcctg agaatgctct
     3121 agctgcaatc catcggggaa aagttgttgg taaatatgtc aaacaccatc tacctaacta
     3181 tggcgtcttt gatgagttta gaaattttgg gcagggagat aagagcctag taattcgctt
     3241 tggctctatc gatatcgcgc tagcaatctg tgaagatatc tggcaagaag gcggcccagt
     3301 tgccaattta agcaaggcaa atcctggaat tttgctagtt gccaatggca gcccttttga
     3361 gcgggataaa gatggagtaa ggctagatct tgcaagaaag agagttaagc aactgggctg
     3421 cccgctggtc tatctaaata tggttggggc tcaggatgat ctagtttttg atggcggttc
     3481 atttgcctta gatagcaatg gcgaggtaat tgcaaaatct gaacaatttg aaagcttgct
     3541 aaatattgtt gatattgaat gcgagataga aagtggttca cctgatctgg ttttaagcgg
     3601 agcaatcaag aaagataaag gggcagcaag aactctgatt gctccatcgc tatctgatga
     3661 ggaagaaatc tggaaggctc tagtagttgg cctcaaagat tatgtatcta agaatggttt
     3721 taaatctgtc gcccttggcc tctctggcgg tattgattca gcgctcacag ccacattggc
     3781 agttgatgcc cttggcaaag agagcgtcta tggcctactt atgccaagta agtattcatc
     3841 aacgcactcg atatcagatg ctaaagagtt ggctgaaaat cttggtattg gctatgagat
     3901 cattgagatt ggctcagttg tcagtagttt tcaaagcgcg attgagctct caggccttgc
     3961 tgaagaaaat atccaagctc gagttagagg ctcgctactg atgggacttt caaatcagcg
     4021 aggtcattta attcttgcaa caggtaataa gtcagagcta gctgtgggtt attcaacgct
     4081 atatggcgat gcggtaggcg gctttgctcc gataaaagat atcttcaaag tagatatctg
     4141 gcgccttgct agatggcgca atagccaggc cgggggcaag gggctaattc cagttaactc
     4201 aattgagaag gcgccaagtg cggagctatc tccaggccag agagatagcg actctcttcc
     4261 agattatgag ctccttgatc aagttttaaa gctttatatt gagaaaaata tcgatgccac
     4321 aaagatcatt gcatcaggct ttgataaggc cttagttgat cgggtggtag caatggttga
     4381 taaagctgaa tataagaggc ggcaatatcc acctgggcct aaagtctcac caattgcttt
     4441 cggtaaagat agacgtttgc caatgacctc aaggtggaaa gagagttaga aaaacttgca
     4501 aagtctttgc aagtagcgct atttattatt tcgcctaaac ttcgtgctat ggcttcgatt
     4561 cgctcctggt tcttcacgcg ccgacgcgta atcgattttg gtcgcagggc atcagcaatc
     4621 tgctgtttgt aatttaagaa ttctcgaaat tcaataagca ttttttgcgc ttaaggcgct
     4681 ttcatttaat tccattttaa ataaatttac ttaggagaag attatgaagg tttatgaaaa
     4741 tatcacccag gtctatggca ataccccgct agttaggcta aatcgaatta cagatggagc
     4801 cgaggcaacg gtctatgcca agttggagtt ttacaaccca acgagcacgg ttaaagatcg
     4861 aattggcatc gctatggttg atgccgcaga aaaaagcgga gctctaaagc ctggtggaac
     4921 aatcgttgag gctacatctg gaaataccgg aatcgcactt gcaatggttg gggcggctcg
     4981 cggctataaa acgattctca ccatgccaga ttcaatgtca aaggagcgac gtacacttct
     5041 tagagcattt ggcgcagagc ttgtccttac tccagcagca gagggaatga aaggcgccgt
     5101 tgctaaggca gaggagctag gagcaaatgg cgcagttatg gtccgccaat ttgaaaatga
     5161 agcaaaccca gaagttcata gaagaacaac tgcggaggag atttggcgcg ataccgatgg
     5221 cctagttgat gcagtagttg ctggaattgg caccggcgga acgatcactg gaattggtca
     5281 ggtattgaag tcaagaaagc ctgaagtgaa gatatttgct gttgagcctg ctgcttctcc
     5341 tattctcaat ggcggaaagc ctggcggaca tccaattcaa ggaattggtc caaatttcat
     5401 tccaccaatt cttgatcgaa gcgtatacga cgaagtccta gatgtaagcg ctgaagatgc
     5461 agtcaagtat gcaaggcgag cagcagctga ggagggaatc cttgctggaa tttcatcagg
     5521 tggtgcgctc tgggcagctg cccaagttgc aaggcgaaaa gagttcgctg gtaagaatat
     5581 tgttgtaatc attccatcat ttggcgagcg atatctatca accattctct atcaagactt
     5641 gatggattca taaaaagatg tttaaagtaa ttagccaaga tctagagaca gcgctattaa
     5701 aagatccggc tgccagaaat aagttagaag tatttcttac ctatcctggg gttcacgcgc
     5761 tctggggtta tcgcatctgc cattggctct ggaatagaaa attaaagcta atctctcgga
     5821 tctattctaa ttggattaga agtattactg gaatagagat tcatccggca gccaagattg
     5881 gtaaacgctt ctttatcgat cacggtatgg gggtagtaat tggcgagaca acggtaattg
     5941 gcgatgatgt aatgatttat cacgatgtca ctcttggcgc tagaactttt gaaaatggca
     6001 aacgccatcc aactattggc aacagagttg ttattggagc gggagcaaga gtccttggcg
     6061 atatcaaaat tggcgatggg gttcgcatca gcgctaattc tgtggtggtt aaagatgttt
     6121 caccgcagag tgatatcgat gcctcagagc aatttgctat ctaaggctca tttttaagcc
     6181 tagttaacat agccgtaact aaaggcgggc attatgcgcc catggctaaa gaggatattg
     6241 agatagcaaa gcagaaggaa ttcgttcttc gcaccctaga agagcgcgat attcgctttg
     6301 ttcgtctctg gtttactgat gttctgggat ttctaaaatc tgttgcgatt gccccgccag
     6361 aacttgaaaa tgcctttgct gaaggaattg gctttgatgg ctctgcaatt gagggttttg
     6421 cccgcggcag tgaatcagat atgttgctaa aaccagatcc tgcaacattt tcaattcttc
     6481 cgtggcgaac tgaaaaacca ggggcagcaa gaattttctg cgacatcgca cttcctgact
     6541 cggctccatc tccagctgat tcaagaaatg tcttaaagag agttttagaa aaagcagcca
     6601 agatgggata cacctgctac acccatccag agattgaatt cttcctattt aaagatgcgc
     6661 ctgctttagg agtaaaacca actccagttg atgctggtgg ttactttgat caaactccaa
     6721 ttgctatcgg tcaagatttt cgcagacagg caattactct gctagaggcg atgggagtct
     6781 ctgttgaatt tagccatcat gaagtagctc caggccagca ggagatagat ctgcgatatg
     6841 cagatgcgct aagtaccgcc gataacataa tgacatttcg ccatatcatt aaagaggtag
     6901 cactggatca aggctgtcat gcatcattta tgccaaagcc ttttaccgaa caccctggtt
     6961 caggaatgca tactcacttc tcactctttg aaggtgagaa gaatgccttc tataaagatg
     7021 gatcaacaag tggtgagcta tcaaatgttg gcaaatcatt tattgctgga attctcaaac
     7081 atgcgccaga atttattgca gttactaatc aatgggttaa ctcttataaa cgccttcatg
     7141 gcgggggaga ggcgccagca aaaataagtt ggggcgcgca aaatcgcgat gcgatgataa
     7201 gaattccgcg ctataaacca aataaagaaa actcaaccag aattgaattt agagccccag
     7261 attcagcttg taatccttat ctagcttttg ccgtcactat tgcggcagga cttaagggtg
     7321 ttgagagcgg ctatgaatta ggcattgatg agagtccagc aagccttcct gccgatctta
     7381 atgaggcgat tttggcgatg caggagagtg aattggttag gcagtgccta ggtgagcatc
     7441 tctttgatta tttccttcgt aataagcgag ccgaatggct ggagtactcc aagcaggtta
     7501 cagcctttga acttgatcgc tatttgccag tgttataagg ccttggctaa ccttaggccc
     7561 atgttacgca gcgaattatt acttctagct cttctgagca cactccttct tcgctgccgt
     7621 ggcgaggcca aataaccggt ctcctcgctt cggggtttta acgcaccggt tatcggtagc
     7681 aatgaaaccc aataacaatt aaggagtaaa gaagtgaatt ttccaaagca agaagcctca
     7741 aagatggcta agcatcgcta ctcatctttt attccaatca aattagatga tcgcacttgg
     7801 ccaagtaaaa ccatgaaaga ggcgcccaag tggtgctcag ttgatttaag agatggcaat
     7861 caagccctca ttgatccaat ggatgtccca agaaaattgg cgatgtttag cctacttata
     7921 aagatgggtt ataaagagat tgaagttggt tttcctagcg caagccagac tgactttgat
     7981 tttgttagaa agattattga agatggatta attccagatg atgtaacgat tcaagttctg
     8041 acccaggcta gggatgcttt aattagaaga acttatgaat ctctagtggg agcaaagagc
     8101 gcggtagttc atctctataa ctccacctca acacttcagc gcagagtcgt ctttggacag
     8161 gataaagaag gaattaaaaa gattgcagtc gatggagcta agttatgtaa agagttagtt
     8221 tcaacccttc cagataccaa gatctccttt gaatactcac ctgagtccta caccggcact
     8281 gagttggaat ttgccctcga ggtctgtaac gctgttaatg acatctggca gccaacccca
     8341 actcataaga gcatcattaa tctgcccgca actgtggaga tggctacccc taatgtttat
     8401 gccgattcga ttgagtggat ggggcgtaat ttaaagtatc gagattccat tgtgctaagt
     8461 cttcacccac ataatgatcg cggaaccggc gttgctgcag cagaacttgg atatcttgcg
     8521 ggagcagatc gcattgaagg aacgctattt ggcaatggcg agcgaacggg aaatgtctgc
     8581 ttagtaacac ttgggcttaa tttgttaagc catggcattg atccgatgat tgactttagc
     8641 gatattccag agattagaag gactgtggag tatgtcaatc aattaagagt tccagagcgt
     8701 catccatatg gcggcgatct tgtctttacc gcctttagcg gttctcacca agatgcaatt
     8761 aagaaaggct ttgagcatct agaaagagat gccaaggctg ccggcaaaaa agtcgatgat
     8821 ttcacctggg cggttcctta tctgccaatt gatccaaaag atatcggcag aagctatgaa
     8881 gcagttatca gagttaattc tcaatctggt aagggcggag tggcttatgt gatgaagagt
     8941 gaacatcaat tagatcttcc tagaaggctg cagattgaat ttagccatgt gattcaatct
     9001 cataccgatg atcaaggcgg agaagtattt ccagatcaga tgtggaagat atttgtcgat
     9061 gagtatctgc caagtgaaga taacaagtgg ggaagattta gatttacatc ccttaaccaa
     9121 agtagctctg ctggcaaaga cgttgagtta agcgttgatc ttcttgataa tcaaaagcag
     9181 ataacactta gcggaagtgg taatggtcct atctctgcat tctgtaatgt gatgcaaagc
     9241 tatggagttg atatccaagt agctgattac tacgaacatg ctatgagcgc tggtggggat
     9301 gcaagcgctg ccgcctacct ggagtgcacc gttaacgggg gaacttattg gggcgttgga
     9361 attgatccat caacgactac cgcatcgcta aaagcggtga tttctgcggt caatcgcgct
     9421 cttcgataac catctgctct aagttgtgcc tgtggcgctc tatcgtgatg aggcagttgt
     9481 tatcagaacc cagaagcttg gggaggcaga tcgcattgtc acgctctttt caaggcagcg
     9541 cggtcgtata agagctgttg caaagggagt tagaaggact aaatcaaagt ttggcgcaag
     9601 acttgagcca gctagttatg ttgatatcca gctctacacc ggaaaaactt ttgatgtcgt
     9661 tactcaagtt gaagctattg aaaattatgg cgatgcaatc tctggtgact atcagaggtg
     9721 gaccattgcc tctgcgattt tagaaactgc tgagcgcttt acaaataacg agggagagcc
     9781 agcgctccaa caattccttc ttctaactgg cgcacttaaa tcacttgcgc atcagagcca
     9841 tgatccatca ttaattcttg atgcctatct acttcgctca ctgtcaattg ctggttatgc
     9901 cccatcgatg acaatttgct caaggtgtga agcgccaggg ccacataaat acttctctct
     9961 agttggcgga ggatcagttt gtttagattg tcgtccctca gcttcggcaa caccagcact
    10021 tgaaactctg caattaatgt cagatttagt aagtggcgat tggaagtcag ccgagcagag
    10081 cgagctaaaa aatcgcagag aggcctcagg attaattgct gcatacttgc aatggcatct
    10141 agagcgaaac ttgcgttcac ttcccatggt tgaaagagtt atcttgtgaa agttccaaaa
    10201 attgctaaag aaaaaatccc tcaccatgtg gcacttgtta tggatggaaa tggtagatgg
    10261 gcaaaagcgc gcggccttgc aagaacaaag gggcatgaag ctggggaagc tgctttattt
    10321 gatgtagtac agggcgcaat tgaaattgga gttaaagagc tatcagcctt tgctttctca
    10381 acagagaatt ggcggagatc tcctgatgaa gtgaaattct taatgggctt taatcgcgat
    10441 gttattaggc gcaggcgaga tcagatgaat gagatggggg taaggatccg ctgggttggc
    10501 gctcctaagc ggttatggaa gtcggtaatt gaagaacttg agattgctca agagttaact
    10561 aagaagaata aagtcttaac cttaaatatg tgtattaatt atgggggcag agcagagatt
    10621 gcatctgcag ctcaggaaat agcaagagat ctagcaaaga agaaaatcaa agcagatcag
    10681 ataaatgagc gtctctttgc taaatatctc gatgagccag ggatgagtga tgtagatcta
    10741 tttctaagat cctctggtga gcagaggaca tcaaactttt tgatctggca gtcggcttat
    10801 gctgagctag tctttatgga tgtgctctgg cccgatgtga ctaggcagac gctctggcag
    10861 gcaatagaga tttactccaa acgtgaccgt cgattcggaa aggcttgaaa tgagtaaaag
    10921 taatgtgcag ttctggtttg atccaatctg tccttgggca tttatgacat cgcgttggct
    10981 attggaagtt gagaagataa gagatatctc tattacttgg aatctattta gtcttgccca
    11041 cctcaataaa gataaggatt tagcgccaga atataaagca aggctaattc gctcctggca
    11101 agcaacaaga gtcattgctg ctgcgcaaga gaaaaacggt aaagagataa cacttccgct
    11161 ctataccgcc atctcatcaa gaattcactt aaagaagatg gaagtgggta aggagttatt
    11221 cactcaagcg cttaaagaag ttggcgcaga tgaaaatcta gctagcgcag agagtgactc
    11281 caaatttgat aatgcaatta ttgctagcca tgaggctgga atagctctgg ttggaagtga
    11341 tgtgggaact ccaataattg cagttgaggg aacggcattt tttggtccgg ttatttctcc
    11401 tgctccaaag ggagaagagg ctggaaagtt atgggatgga gtattacttg cagcaagtta
    11461 tccaggattt tttgaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
    11581 aaggagcgag acagtgttta atccatcgct aaatgcgtgc acaaggagag ctacaaagac
    11641 cgcaatccca agtttttcat caattgtgaa agcaactcca agtgctaggc catcaagaaa
    11701 tacatggcca gccatcgcaa aagctccaag gcctcccgca acatttacgc gatgcttatg
    11761 gtcatcgctg aaatcagact ctgcaggttc atgagttcca aagaacttct catatagatg
    11821 aagaagtaaa aaaccaccaa ctagagctac tgaaactgct ggagctccaa gaagtgttgc
    11881 ggaagacatt tcaaagactt caggcaagag atcaaaggcg acaagaccta gaagaagtcc
    11941 ggcggcaagg ccaagaacta gatggaggcg atctttagcc tttagtgcaa gcaggccacc
    12001 agctgctgtt gctaatgcgg tcgcagcggc aagaaggata ggtaggttca tggggcagaa
    12061 gttagcacaa atgagaatca ttttcattaa gggggttaag ggatttaagc gtgtctcggg
    12121 gggtaaaatt tggctcctgc gcccagcttc ctttgtgaac tgcgaccggt gcgcaccgcc
    12181 tgcagccagc cggatggaga ccaccatggc agatcgtctt gaagtaattg ttagcctggc
    12241 caagaggcgc ggctttgttt ttccctcatc tgaaatctat ggcggtcttc gcgcatcttg
    12301 ggattacggc ccacttgggg ttgagttaaa aaataacgtt aagcgccaat ggtggaagtc
    12361 aatggtgaca ggacgtgagg atgttgtcgg tcttgattcc tgcgtcatat tagctaaaga
    12421 agtttgggaa gcatcaggac acgttgaaac ttttagtgat ccgctcactg agtgcaccga
    12481 gtgccataaa cgatatcgcg ctgatcatct agaagaggct tatgaagcaa agcatggaaa
    12541 gaagccaaca tctttatctg aaattaactg tccgcattgt ggcaacaaag gtaagttcac
    12601 cgttccaaaa caattctcag ggctattaaa aactttccta ggaccagttg aggatgaatc
    12661 aggtcttgca tacctaagac ccgaaactgc tcaaggaatt tttattaact ttgataatgt
    12721 ggcaacaaca gctcgaaaga agccgccatt tggaattggc cagattggaa aatcttttag
    12781 aaatgaaatt actccaggaa atttcatctt ccgcactaga gagttcgaac agatggagat
    12841 ggagttcttt gtcgccccag gaagtgatga agagtggcat caaaggtgga ttgataccag
    12901 acttgcttgg tatgttgatt tgggaattaa tccgcagcgc cttcgtctct ttgaacaccc
    12961 taaagagaaa ctctcccatt actcaaagag aactgttgat attgaatata actttgaatt
    13021 tgccggaacc caatggggag agcttgaggg aattgccaat cgaaccaact ttgatctaaa
    13081 ggcccactct gaaaagtcag gcaaagatct ctctttcttt gaccaagaaa aaaatgagcg
    13141 ctggtttccc tatgttattg agcctgcagc aggcgttgat cgctgcgccc ttaccttctt
    13201 aatggatgcc tatagcgaag atgaggcgcc taattccaag ggggaaatgg agaaaagagt
    13261 tgttctccgt cttgattatc gattagcgcc agtaaaggtt gcagttcttc cgctatctag
    13321 aaatgcagac ctctcaccta aggcaagaga tttagctgca gcgcttcgca agaactggag
    13381 cgttgacttt gatgatgctg gagcaattgg ccggagatat cgcagacaag atgagatcgg
    13441 tactccttat gcaattactg ttgactttga tactttaaat gacaacgcgg taacaattag
    13501 agaacgtgat tcaatgaagc aggaacgaat cagtattgat caggttgagg catggttagc
    13561 cccacggcta ctgggctgct agatctaaag ccgcttcatc ttccaattcg tggtggcggt
    13621 ttagatttaa ctgcgccagt tgttcttgct ccaatggctg gcattaccaa tgctgctttt
    13681 agattgcttt gccgggaaca aggcgctgcc ctctttgtct cagagatggt taccgcaaga
    13741 gccttaattg aaggtaatga tgaaacttta agaatgatta cgccaggagt tggtgagcat
    13801 cctcgctcta ttcagctcta tgccacaact gcagaggatt tgtcacgagc agttgagatt
    13861 ttaggaagta gagatttagc agatcatata gatctaaatt ttggttgccc agttccaaaa
    13921 gttacaagaa aagggggagg ttcagcactt ccctataaga ggcgcctctt tgcatctcta
    13981 gttgaagctg cagtaaaggg cgcaggtaag tatcagattc cagtaacagt gaagatgcga
    14041 gttggaattg atagtcagta tgaaacttat ttggatgctg gcaaaattgc tgcagatcta
    14101 ggtgttgcat gggtggcact tcatgctaga actgccgcgc agctctatga aggaaaatct
    14161 gattggagca aaatcaagaa cctagtagaa caccttgctc caactggcat tccagtgctt
    14221 ggaaatggcg atatctggtc aggctctgat gcgatagaaa tggtgaaaac aactggatgt
    14281 gcaggcgttg tagtgggcag agggtgtctg ggacggccat ggttatttgc agatttagtt
    14341 gcagccttta atggccaaga gctaagagct cttccaactc tatttcaggt tagagagatt
    14401 atttatcgtc atgcagagct tcttactcag tatttctcaa gtgaggatag agcttgtaga
    14461 gatttaagaa aacacatggc ctggtattta aagggcttta gagttgaaag agagcttcga
    14521 gcaaaatttg gaatggtttc atcacttctt gaacttcgcc aacttttaga tcaattagtt
    14581 gatcaaccat atccagttgc aattggtgat gcgccgcgag gacgaaccag ccatggacgc
    14641 gctgtgagcc tgcctgatgg ctggctcaat gatccagaag aattagttac agttgatggg
    14701 caagattctg gatctggcgg ctgatgttct tatttaaatt tgcttggaag tttattagct
    14761 ttctacttct agttgccttg gttattccac tttgggcaat tggaaagact tggtataccg
    14821 cgcagaatga aaccattaga aaagcagatg cgatagttgt catgggggcc gcgcaattag
    14881 atggaagacc tggtgaagtt ttagaggcaa ggctaaagga gagtcttcga atatatgaga
    14941 agggctttgc cacagagatc tacaccttag gtgcgggagc gccaggagat cgatttacag
    15001 aagcagcagc agcaagaagt tatttgattt ctaaaggagt aagagcaaag agtattacag
    15061 ctgttgccaa aggccgtgat acttatcgaa gcatcgaagc cctatcaaaa gctgagggca
    15121 taaagagaat aaaagagata attattgtta ccgatcctta tcactgctta agatcaatga
    15181 cgatggctaa tgattttgga tttaacgcta cctgctcacc aacaagggtt ggcatcgcta
    15241 gcttggaaga agctggattt cgctacttat tgcgagagag cggggcctat cttgcatatc
    15301 taacccttgg gcgccgtggc atacatatca gcgaccacgt agcctaagca ccttatggcc
    15361 cttcatcctg caaacgagca tcttggttat agcgagctgg accgcgaaag atttctcgat
    15421 gagccagcaa agcgcaaggg gcgcaccgaa tttgccagag atcgcgccag aattattcat
    15481 tcatttgctc taaggcgtct tgccgcaaag actcaagtag cagttccttg ggcaagtgat
    15541 tttccaagga cgagactttc tcactcactt gagtgtgcgc aagttggtag agaaattggc
    15601 gcagcacttg gcgcagatcc tgaccttcta gaaagtgcct gtctggctca tgatttagga
    15661 catcctcctt ttgggcataa cggcgagaaa gcacttgcaa agattgctaa agatatcggc
    15721 ggttttgaag gaaatgcgca gagctttagg ttattaactc gaattgaagc aaagactgtt
    15781 gatcaaaatg gactctctgt tggcttaaat ctgactcgag caacccttga tgcggcaaca
    15841 aaatatcctt ggagcagcaa agaaaatcca gaaaagtttg gcgtttatca agatgactta
    15901 gagatattta attgggttag acaagaatct ctagaatcaa gaacatgcat ggaagcccag
    15961 attatggatt ggtccgatga tgtggcatat agcgttcatg atctagaaga tggcctggtt
    16021 actggacaga ttgatatttc aaagttgaat gacgatctag agcatctcta tcagactgca
    16081 attagagatt acctacctga tttgagtgag caagaagctg catctgctct taactctttg
    16141 cataatcttt catgctggcc aaaagaattt gatagaagcc atagacacct tgctcagtta
    16201 aaggatctga ccagtcaatt aataggtcgc ttcgctctct ttgcagagcg agccacaaga
    16261 gagagatatg gcgaggggaa tttaactcgc tatcaagcaa acctgattgt tcctcgtgag
    16321 gtaagaattg aggtagcgct gcttaaatca atggcgggtt actacataat cggcgcagaa
    16381 gtatccaagg agcgttatgc aagacaagaa gaggtaatta ttgaattagt tgaggctgtc
    16441 agccgccttg ccccagcaag tcttgagagt ttcttcctcc agcaatggca ggttgcagat
    16501 agcgctgagg caaaaatgcg cgtagtaatc gatcaggtag cttcattaac agatatgggt
    16561 gcatacgccc tccatgagaa attgattggg gcttaggatt acagctatgt ctggacgaat
    16621 tcgcgatacc gatgtgagct atgttcgcga tcattcacca attgatgaag ttgtcggtga
    16681 ttatgtccaa ctaaaaagcg ctggtggggg acagaagaaa gggttatgtc catttcatga
    16741 tgagaagagc ccgtcatttc atgtaactcc tgctcgcgga tattttcatt gctttggctg
    16801 ccaagagggc ggggatgtca tcgcctttat tatgaagatt gatcatttga gttttactga
    16861 aactattgaa agattagctt caagaattgg ttatcaatta acttatgaac aaagcagtgg
    16921 cggatcaaag gctccaacag ttcagcgctc tagacttctt gaagcaaata gccaagcttc
    16981 ccttttttat caagagcaac tacaacttcc taccgcaagc catgctagag agctactaat
    17041 taaacgtggc tttgataaaa cagcgagcca atcatttgaa attggctacg cccctgatca
    17101 atgggatgcg ttaagtaaat tcttacttgg aaaaggtttt agcgcagagg aacttctgct
    17161 agctggatta acaaaagagg gaaccaaagg agctattgat cgctttagaa atcgcttgat
    17221 ttggccgatt agagatatct ctggtgattt agttggtttt ggcgctagga aattagcaag
    17281 tgatgaagaa gatactggtc cgaagtatct aaatacccct gagacttcga tctataaaaa
    17341 gagccaagtt ctttatggcc ttgataaggc caagaaggag atagcaaaga agcgccaggt
    17401 agtaatagtt gagggctata ccgatgtaat ggcagcgcac ttagcaggaa taccaactgc
    17461 cgttgcaact tgtggaaccg cctttggtga tgagcatata agaatattgc gaaggctatt
    17521 gatggatgat gatgcattcc gcggcgaggt gatctttaca tttgatggtg atgctgcagg
    17581 acagaaagca gcgctgcgtg cttttggcga tgatcagaaa tttgttactc aaacctttgt
    17641 tgctgttgag ccaagtggat tggatccatc tgatttaaga caagaaatgg gtgatgctgc
    17701 acttcgcgat ctgattgcaa ggcgggtccc tctttttgag tttgcgatca agagctcaat
    17761 aaagcaattt gatttaggaa atgctgatgg cagggtgcaa gcgctaaatg cagctgcgcc
    17821 ccttattgga aagattcgag atgcctcgct aaggccagag tatgcaagat cacttgcggg
    17881 ctggcttgga atggaagttg aagttgtcat ggctgcggtt aagaaaaata cttcaactaa
    17941 gccggcaaat aatgatgctg tggtagctgg cgagcaatgg cgaccagatc caagtgagcc
    18001 aagattaatt cttgagagag aagttttgaa ggttcgcttg cagatgccag cgctagttag
    18061 aaattggcca gagctggaaa aaaatgcctt ctcacatcct gcctatataa agctaagaga
    18121 attcattgat acccaagagg atttaatggc agttaattta gaggcgacag attctgatga
    18181 acttaaatca ttcattaccg agttaatggt tgaaccaata cgcaccgatg gtgaaatctc
    18241 ggatcgctat gtttcaagta ttactgccag attaaacgag gtagcacttg ccagatctat
    18301 cgctgaagtg aaatcaagcc tccagcgatt aaatccgctg gagagtgaaa gtgaatacaa
    18361 tcagatgttt tcaaggttag ttgagatgga ggcaaagagg cgggcccagc gagaattggc
    18421 gcttggggag tctctgacct agatttagcc gctgaacgat ccctgatagc tcaattggca
    18481 gagcagccgg ctgttaaccg gcaggttctt ggttcgagtc caagtcaggg agcttttcat
    18541 ttagactcat ccctatggca atggtccgta ttcgtgtagc acttcctgat cgcccaggat
    18601 ctttaggcgc tgttgcatct gcaattggtt ttgctggcgg agatattcgc ggccttgttg
    18661 ttctctcatc tgaaggtggg cgaggaatag atgacattac cgtggcattt ccaggaagtg
    18721 acccgcaaga tttagtaaat gtattaagcg caattggcgg cgttgaagtc atctcagtaa
    18781 cgccggtcgt ttaagaaata actttagctc catcacttgg tagcgaatca aaaaatctag
    18841 gcgctttaaa tccactttga gcaaaagctt tctcaattgc tagagaaatt gctcctgcat
    18901 cgctttcatt aattagtgca atagcactac cgccaaaacc gccgccaacc attcttgccc
    18961 ctagggctcc agagtcaagt gcggtatcaa cggctagatc taattcaggg catgagactt
    19021 cataatcatc tcgaagcgag ttatgagatt gatttaataa ctgaccaagt gctttaaaat
    19081 ctcctgtctc aagggccttt gctgcatcta aaactcgggc catttcgctt acaccatgtc
    19141 gcgctcgttt aaattgaata tcacttaact ttgttttatg ggctagcagg ctatccatag
    19201 ttaattggcg cagggagtta attgaaaaaa ttttagctac ctcctcgcag ctctctcgtc
    19261 tcttggcata gccaccatca gttagggcgt ggtgggcctg ggtatcaatt attagaagcc
    19321 ttagagaatt tgaagcaaaa tcaattttga tatgcctagt tgagagatcc tggcaatcaa
    19381 gtaatagtcc ataaccagct cttgccatta gcgaaattga ttgatccata attccgcaag
    19441 gcactcggac atactcattt tctgcccgct gggtcaggcg agctaactca ggtaaagttt
    19501 tttctaagtg aaatagatga tttagcgcag tagcaagaga gcactcaagg gcagctgaag
    19561 aagataatcc agcgccaagt ggcacctttc catcgatata gatatcaagg ccagaattta
    19621 tttccatcgc ccagataacg ccataaatat atctagacca ggaagatcca tcttttgcag
    19681 cgattgcttc taaattactt tcaatcattt cattcttctt ctgcgctgaa ataattctta
    19741 tttttctgtc atctcttttg gaaactgcac acatagttac tgcatcgata gcgaagggga
    19801 gaacaaagcc cccactgtaa tcaacatgtt cgccaatcaa gttcactcga cctggagcgc
    19861 ttgctactac tgcaggggga tagccaaagc ctttagtgaa taactctgtt atttccactt
    19921 tgttttaaag cgccacatcg cgcagctgcg ccgcagattg ctctggcttt agatccatca
    19981 taaatgcacc aaatgcagat tcagatcctg ctaaatactt taactttcct ggcgctcgtc
    20041 taactgaggc tatctgccaa tgcagacgcg ttacatctct tcccactctt accggcgctt
    20101 gatgccagga ggcgatgtag ggcatcttta caccaaagac gccatcaagt cttagtaaaa
    20161 cttctttagc aagaggagca aatgcatctg atgcagcttc atcaagagct agaaaatcag
    20221 gaacgcttct tcttggtgca agatgaattt caaatggata tcttgaggca tatggcacat
    20281 aggcaaccca ttcctcattt tgagcaacta tgcggacttt atctgaaagc tctctagcta
    20341 aaatatcttc aagaagaata tttccagtct tctttttgtg agcagttgct actgccagcg
    20401 ctctctctgc ttttggaggt agataggagt aggcataaat ctgaccatga ggatgattaa
    20461 gagttactcc aacttcctcc cctcgatttt caaaagcagc tatgtattca atgtaggaaa
    20521 gctccgagag ctctgcagtg cgatcgcgcc aagcctcaag aacagttctt atctggcggt
    20581 gtgagagatc tttaaaagag gcctcgtaat ccgatgtaaa acagataact tcacatttgc
    20641 ctgctgaatc aatcgctggg ctcgaaaagt ctggattatc tatcgcctca aaacttgatc
    20701 tgggagcggc aagtgatggt gagcggttat caaagacaac cacttcataa tcactttctg
    20761 gaatttcagt aagtgaacct ggcttacttg gagccagtgg atttaattct ttaggtggca
    20821 gaaatactcg ttcctggcga tgggctgcaa taactaccca ttcattattt aatggatcaa
    20881 ggcgcatctc accgatgcct ggttgctctt cactgcttcg ctgatctact gcggtgcgtg
    20941 attgaccctt tgtgtcgtag tagcggatgg tgcgcccatc catgagcgtg gcatcacttc
    21001 gaataacgcc tgcggggagt ttcttactcg acataatggc tattactcta cccgtatgtc
    21061 ttctccaata ccttcatcta aggccggggc cagggcgcag ctaagcaatg gcggcgttga
    21121 tttactcctt gaggttatag atggcgcacc ggtcattaga tattggggcg caagttttaa
    21181 gggcgaggca agtgaactac tttttgatcg ctccattgca aatagtgatt ttgatgcagt
    21241 tgctaatcca ggaatgatgc gagagcactc aagaggacat ttgggatatc ccacaataaa
    21301 gggacataga gcaggtcttg attggtcaac aaaatttagc gtcaaagaat taaaaagcga
    21361 taaaagtagt tttcaaattc aattagaaga taaggatgcc aagttaactc ttgatatcgg
    21421 cgcatcccta gatcaatttg gagttttaaa gctctggcaa gaactaacta accttggcga
    21481 tacttatttt ctagatgagt taaattattg gatgccgctt agtgatagtg cgacgcagag
    21541 cttagatttt gttgggcgct ggtctaatga gcgcaatccg cagcggcgag atattgcaat
    21601 tggcagatat gttcgagata gccatgaggg acgacctggg cataattaca cgataggaat
    21661 gattgcttta gagaaagaaa ctaattttgc atcaggacaa tcttgggcgc ttagccttgc
    21721 ttggagtggg gatagccaat attgcattga aaaaaattat gagggaagtg caagcatttc
    21781 ggccggagaa gtacttcttc caggcgaagt gattctcgaa aaaggtcaga gctatagagc
    21841 ccctgatctc tttgctctct attcaaatgc tggccttgat ggactttcct caaatatgca
    21901 taagcattta agagcaagga gtgtgcatcc aaaaaatcct cgtcctatga cgctgaattt
    21961 gtgggaggca atctactttg accacaatga ggagaagata aagaagattg ttgatacagc
    22021 agctgaagcc ggctttgagc gcgttgtctt agatgatggt tggtttggct ctagaagaag
    22081 cgatctaagt ggccttggtg attggcaggt aagcaaagag gtgtggccaa gcggtcttgg
    22141 atcacttgcc aattacataa gagataaagg tatggagttt ggcctctggt ttgaaggcga
    22201 gatggttaat cctgattcag atctctatcg ctcacatccc gattggatat taaaggttgg
    22261 cgatcgggta ggtccaacgt ggcgccatga gttggtcctc aatcttgata atcaagatgc
    22321 atataactat gtgttggatt cagtagacaa aattcttact cagtaccaga ttacctatat
    22381 caagtgggat cacaatcgag ttcttattga tgcgggctca aataatcgag ctgcagtaag
    22441 aaagcaaact caaggacttt atcgtctctt tgctgaacta aagaagcgcc ataaaaatct
    22501 agagattgaa tcttgcgctt cagggggagc aagaattgat ctaggaatta ttgatttagt
    22561 cgaccgattc tggaccagcg acaataatga cgctcttgag cgagccagaa ttcagagatg
    22621 gagcgcgcaa tttattccgc cagagttgct cggaactcat attggagcag atcctggtca
    22681 tcaaactgga agatctatct cattttcctt tagagcttta actgctctct ttggccacgc
    22741 aggtgtggag cgagatatca cccaactttc tagtagtgag ttagctgagc taaagagttg
    22801 gattgctctc tataaagcaa aacgcaaact tcttcatagc ggggatgtta tccgcatcga
    22861 ttacccaaag gattctcact atctcaatgg cgtcatttct agtgataaga aatcagcgct
    22921 attttcattc gcatgtcttg agaccattcc agagtctcac ccgccgcgca tgcgcataag
    22981 agggcttgat tctgatcgca gatatagcgt taaggtttta agtggaggtc aaaaatcaaa
    23041 tttcatggct atcgagccgc ccatatggat tactactggt cttgaattaa ctggatcaga
    23101 gcttgaaaat ataggtctta gcgcgccaat cttgcgccca gcatcagcgc ttttaattga
    23161 aataactgcg ctttaatcaa gccagtttag agtcttttca atggctttct tccaattctt
    23221 aaatcctgta gttgcaagcg tgctggcttt atctggttta aagcgcttat cttcgcgcca
    23281 cttggctttt agctcactag tatctttata aaaacctgtt gcaagtcctg ctgcataggc
    23341 agcgccgagg gcggtagttt caattatcac cggccgaatt acttcaactc ccataatatc
    23401 tgcctgcatc tgcatacata actcattagc agtaattccg ccatctactc taatttcagt
    23461 tagagcaact ttgctatcag cgctcatcgc atctaataca tcgcgggttt gataacagat
    23521 tgcttcaagt gctgctctgg caaggtgagc tttggtagca gctctactta atccaacaat
    23581 tacccctctt gcatcacttc gccagtaagg ggcaaagagt cctgaaaatg caggaacaaa
    23641 ataaaccccg ccattatcac taacgctctt tgctaactct tcaacttggg gagctgaggt
    23701 aatgatgcct aattgatccc taagccattg gattgcagat ccagtaaccg ctacagagcc
    23761 ctcaagtgca tatcgcgctt tgtcgtcacc aaatttatag cagagcgttg tcaaaaggcc
    23821 atgcttagat ctaacgatcg atgttccagt atttagcaga gcaaaatttc cggtgccata
    23881 ggttgtcttt gattctccaa gttcaaaaca agtctgtccg accatcgctg cttgttggtc
    23941 gccaagaatt ccagagatag gaactgagct tgcaagtggt ccattactca agctatggcc
    24001 ataaacttgc gaagaagatt tgatttcagc aagtatctga cgaggaattt caaagatttc
    24061 taacagctca ctatcccaat caaggctctc aaggttcata agtagagtcc tgctggcatt
    24121 ggtcacatcg gtgtagtgga ccccaccatt tactccaccc gttaaattcc atagcagcca
    24181 agaatctatg gttcctgcca gcgcccttcc atcctttatt gccagggcaa cttcgggaga
    24241 attcttaatt agccaattaa tcttgcttgc tgaaaagtat ggggcgatag caaggccggt
    24301 tttatagacg atcttttctt taacctgatc acttaaggcg tttagaaact ccgccgtcct
    24361 agtgtcttgc cagactatgg cgttatgaag gggcagtccg gtgctcttat cccagataag
    24421 agtggtctct ctctgatttg ttattccaat tgagttcaaa tctgagcctt gaataccagc
    24481 cctctttaac gcaccagtaa taacgctgat gcatgcccgc cagatttccg caccgtcatg
    24541 ttctacccaa cctgctttgg ggagaatttg agaatgttct aattgttctt gtccaacgat
    24601 ttgaccagtt tgattaaaga ttatgaaacg agtgcttgag gtcccttggt cgagagcgcc
    24661 gatgtagccc atagcccgta tcctaagggc gtgtccagat ctaattccag cctgaatgtc
    24721 gcctctcgaa gcgctgcaat tactgcattg ggtgagagcg aatttgatat tttggtaatt
    24781 ggcggcggaa ttaatggcgt gggaattgct cttgatgctg catcacgagg gctaagcgtt
    24841 gctttagttg aaagcggaga cttcgcatca ggaacttctt ccaaatcttc aaagttaatt
    24901 catggcggac ttcgctatct tgaacaatat gactttcgtc ttgtaagaga ggcgctatat
    24961 gagcgagagt taatggtaac tactctctca cctcatttag ttaagccagt ttcattcctc
    25021 tatccactac atgaaaaact aattgagaga acttatgtgg gagctggaat ggctctctat
    25081 gacgcgctac gaggctttaa gagggcgctt ccttggcata aacacttatc ccagaagaag
    25141 gtaagtgaga ttgcgccatc actgcgcctt gatgtaataa ctggaggatt tcaatatttt
    25201 gatgctcaag tagatgatgc tcgccatact atgagcattg ctagaaccgc tgctaagtat
    25261 ggcgcagtta ttaccacaag aactagatgt gaagaggtca ttaaaaatgg taagcgagta
    25321 gttggcgcaa agattagaga tctattaagt gatgaggtca ttgaagtccg cgcaaaagcc
    25381 acgattatgg catcaggggt ttggagtgat cagctccatg agaagtttgg actaaaagct
    25441 ggttataagg ttcgcatgtc gaagggctca cacattgttg tgcccaagag cgcgattaac
    25501 tctcaaagcg gcgtaattat taagaccgat gtttcggtgc ttttcattat cccttggggc
    25561 gagcaatgga tagttggaac aactgatact gattaccaag agagcaaaga agagcctctg
    25621 gcaagtagcg atgacgtttc atacataatt aatcaagcta atcgagtact tgagcccaaa
    25681 ctaagacgcg atcaagtagt aggtgtattt gcaggccttc gcccacttgt ttcaactgat
    25741 cctgactcac caacaactac attatctaga gagcatgtcg ttgatagtcc tacacctggc
    25801 tttgtttcaa tcgctggcgg taaatacacc acatatcgcg tgatgtcaga ggatgctgtt
    25861 gatgaggcag taaatcactt aaggcgaatc gttcctgatt caacaactga aaaccttgcc
    25921 atcattggcg ctgaaggtta ttcagtattg ataaataaga ttccaaaact agcgaaagag
    25981 tatggtctat ctgaagaaac catcagacat cttcttgatc gctacggatc tttatttgat
    26041 gaagtcttag ctccagcaaa agaagatact tcactgcttg agcctctaat tgaaggcttg
    26101 ctatatataa aagcagaagc gctctatgcc attacccatg aagcagctct aagtatcgat
    26161 gatttgttat cgagaagaac tcgtattgca tttgaagcaa gtgattctgg gctgagtatt
    26221 actagttact taggcgaact ccttggtaac tttgcaggtc ttggaaagaa agagatcacc
    26281 aaatctatta ctgaatatga gcagattatt atgcgccaaa agaaaaattt agagaaagct
    26341 actcagatgg aaagtaaagt tggttaattc ttaatttgat tcttgcaaga atcaaaaggc
    26401 actactggct tagttggctt tggcgttgga gtaacaacaa cttcaggtcc ttgaatcaga
    26461 ttaattgata agtcttggcg attttctaaa tgcgttccag tttgatcaac aaatgcgaca
    26521 tatccttgcg taagacctgc aggaagattc cttagagtta gagtccagat tccagcccta
    26581 tctcgaaccg ggctctgctc cactttaaca actggccccc cgccctcaac aggacttctc
    26641 ctaaaagcta cggttccagt aacaacgggc tctccgcttg ggcgcttaac ttcaacctta
    26701 atctttactg gcttatcagt actacttgcg ttaacgctgg tgataagcag agtggtcggt
    26761 tccttgatcg gcagatagtc gccaacagca ggggtccaga tacctcttgt taattgaagg
    26821 aatgaggcgc tatctcctct aaatacattt aaatcaaccc ttggtgatgg cactccatac
    26881 ttctcgccaa ttccgcacga tgaatactgc caaatttgcc agagcgatga gcagttagca
    26941 gttgaccaag agtgaacaaa gcagccaaat tcttttctcc ctggctcagc aatcggatct
    27001 gcaggattga ttccgtattg cgctttccat agtggatatt ggcgtaattt ctcacttcta
    27061 accatcgctt gctcaagaaa tgttggataa gaataaagaa atggcttacg gccagttttt
    27121 tcatacatcg tttcaagcca agtttcagcc cagagagtga ccaatggctt tggtgcatac
    27181 ttaacgcagc tgccattgga aacttgcaca cagttatatt ccaaatcaag tgcgtatggc
    27241 agatctttct cggtatagcc accaaggcta gagagtcgcc acagcgcttt ttgagcttga
    27301 gccttagcat caagaattac ggattcttta ttggtggtat taggcaatat tgtgtagtgg
    27361 taataaccgg tatagatacc tgctgcttgg gctgcatcac gatccatcaa gagatactta
    27421 agagcaagtg cgtcagcatc atctcttgta tcagaggctt taatcatcac aaatctaatt
    27481 cctgcgttat acatcttcac aaagtcgatc ggtttatctt gaggatgctg ccagcggctt
    27541 atatcggtgc catgaattcg tccgccagga ccgctaagtg aaattaattc atcaggagta
    27601 gaaatggctg ggttctgctc aatcacaatt ctctttgtct ttgtgatgag tgtttttcct
    27661 ccaataatca cttttgctcg atatgttgcg ttggcagcaa gggctgtagc tcttgcttga
    27721 atcttccagg ctccacttgg ggttgattta gttctaaggc gggtatcact ccaagagttg
    27781 ccaattttta catcaatttt taccacagca ttcttcacct ttggcttaat aacgccatga
    27841 aaagttactt tgtaatcagg aaatgcagga gtctgagtta aattaaagga tgaagttgtc
    27901 gccgcctgcg aaatagggga actgatattg gctagtagcg cccaaatgag taagagaagg
    27961 gatatgcggc ggggagaaat cactcggtaa ttatgacgat acttactcca aaaatctctt
    28021 ctattgcagc ctccaaggcg tggcggtgag aatcttttct cacctgagca taattatgtg
    28081 ctccacttaa cttctcagca tcaatgaagg aaagcttaag ttgattgtta ttaaagctaa
    28141 ccaatctggc atcaaaaaat gccagccatg ccacgcgatt ttcacgctcg agaagatcta
    28201 ggacatcatt ccagcgagat ttaatctcag ctaattccat agttttatgt taattaatga
    28261 tcgcaaagat aatggcggca ataccgataa tccaggtgat agttctgccg aaagactttg
    28321 ctagaaaggc ttttccgaaa tttagatagg tcagggcaaa gatgattaca cctgttcctg
    28381 aaatcaaaac tgtttttaaa acaaggtcgc tagcaccagt ttggtaacgc agattaatta
    28441 ggccaagggc aaagagcgca taggctaagt atctaaggta ggaaagcggg ctcattttct
    28501 gctctccgta acatcctggc tagattgcga tggaagtagc cacgctcttg atggatcaaa
    28561 tgagtactta gctatttttc ctaccggaac aatctcatca taaattggat ctgagacaac
    28621 tgagataatt cttccctcat cagtatccaa ggtgtattca acgtgatttc catagaaaac
    28681 aacattttca actaatcctt cacctttgcc aggttttgaa cttctgctct tggcagcggt
    28741 gaccaagatt gattcaggac gaaccaataa gactggcttg cttgaagaaa gggcttttga
    28801 gtgagcagca accaccattt tcttgccact aacctcgatt ttggccttgt ttgctgcagc
    28861 tttgctaaaa gtttcaactg ctaagaagtt tgagcgccca atgaaatcag caacaaacac
    28921 cgttgctggt ctgcgataaa cctcatcagg tgtgccaact tgctccacaa ctgcatttcg
    28981 cataaccaca atccgatcag agaggctcat cgcttcatcc tgatcatgcg tcacaaagat
    29041 gctggtaata cctagttttt gttgaattga gcgaatctca attcgcattt gcgctcgcaa
    29101 tttggcgtca agatttgaaa gtggctcatc gaataaaaga actttaggtc tgacaaccat
    29161 cgctctagca agagcaactc tttgctgttg gccacctgat aattgattag gggcgcgatt
    29221 tgcaagatgg gaaagactca tggaatttaa tacttcacca accgcttctt ttattgcagc
    29281 tgccgacatc ttcttaatct ttagaccata ggcaacattg tcaaagacgc tcatatgagg
    29341 aaatagtgca taagattgaa aaaccattcc catcgggcgc ttatctggag ttagtgaact
    29401 tagatcttcg ccatctagaa gaacttttcc actagttgga gtttcaaaac cagcaatcat
    29461 tcgaagtgca gttgttttac cgcagccaga tgggccaagc agggtgacaa actcaccagg
    29521 attgatagtc agtgtgagtt cgcgcactgc cactaccttc tctttgtcat taccaaactc
    29581 cttggtaagg gcatcaaggt ttagtcttcc tgatttattc ttcattattt ctccttagcg
    29641 tcctgtcacc atggggcgtt ctattgaaac ttgacttcta cgtactagta gattgattaa
    29701 gctgccaatt actaaaataa ttaaaatcaa aatacagcag agggcaaaaa cattgccata
    29761 tctaccctgg tctgcctcag ccagaatctt ctgggtaagt attgatgtat ctggggttac
    29821 caggaagatg atgggagata gcgtggtcat cgagtgagca aaggcgtaca taagtcctga
    29881 taagaatgct ccgctaatga gcggaagggt aattaggcgg aatgtcttac cgccaggtgc
    29941 accaagtgag gcagatgctt cctcaattga gggatcaatt tgttgcaaca tagaaatacc
    30001 agagcgttgt cctgccggac tagatctaat gatgtagacc ataacaattg caatagagcc
    30061 accaagtaga gctgccccgc ctgcaacttg aggaaatact gttacgccag caacttttac
    30121 cggatcgtta taggtgatgg catatccaat accgataact gttccaggaa ctgcgatacc
    30181 caacattcct aggaaatcta gcgcttctga acctcgctta acttttctaa tgactagcca
    30241 agcaatcaac ataccgaata ctcctgctag gggagtggct attagagcaa gcatcgttgt
    30301 tgtcttaatc gctccattgg caaaaccacc gaggagatag cggaagttat ctagagtaaa
    30361 ttcattattt actccaatga tctttacaaa tgccccgaag atcactgttg catagaccat
    30421 aacgataaga gttgtaagta atcctgttac cgagagcaag aagaattttg cgcccctaga
    30481 tgtaactaga gttgggcggc cacttggttt tccggttact gaaaccacgc tcttcttctg
    30541 ggcccaatag cgttggacca aaaatacaag aagtgcgggt aataacagca gtgaggaata
    30601 acctgcagca agtgagatgt cgtaatcacc attaatagcc atataagccc gtgaggcaag
    30661 aactgtgtaa ttacccccga taattatcgg atttgctaaa tcggcaattg attcaataaa
    30721 aagaagtaaa aatgacccag caaatcctgg aatcaaaagt ggaagagtca cagttctaaa
    30781 tattctggcc ttattagcac caaggcttga accagcttca tctaaggcag gatcaaggct
    30841 tcttagcatt cccaagagat tcatgtaagc aattggaaaa aatgagagag ttaaaactag
    30901 aaccagtcct ggaaagccat agagtgtcgg gcgccaatca aatacccctc tagtgatcat
    30961 tccacttcga ccaaagagca cgatgatggc ggtagcaaaa gcaaatggcg gagaaatgat
    31021 tggaattaag ttgatgatat gaagaatttt cttgcctcta aattcaacac gagcttgggt
    31081 ataggccatg atgaaaccca gaactgtgcc aagagctgcc actgtaagac ctagctttac
    31141 ggtattccaa ataatatttc tagtgacaac gtcgctaaaa gatccagtaa tgatggcgct
    31201 gcctgaaggc tgcgtggaat aaatcaagat atttattagc ggcaggatta ttaaaaatcc
    31261 gatgatgaaa ataaccagaa ctagagcaat ctttgtgaag atgtcgtatt tcgctccgct
    31321 ggctttaacg ctcaaaaaac tgctccttaa ctcagattag tagtgagggg ctctgaaagt
    31381 tatttcagaa cccctcgaac tactttactt atattactag gttacttagc tgatgaagtt
    31441 agagctactt ccttatcgaa gcgagccttg agtgcaactg ctgcaactcc tgctgcctct
    31501 gcttgatagt caagcaaagt aactttcttc aagttgacca ttcgagggtc atactttcca
    31561 tttggattgg taagaatctg gaaagactca gcctttgcag gtccaagatt ttgtgcttca
    31621 gccgatagtg agaaatcaat ataagcctta gcagcttctg cattcttggc gcccttaact
    31681 agagcaattc caccaatttc aaagccagtt ccttctttag ggaaggatac gactactgga
    31741 ttgccacgaa gttttgctgc ggtgcagtcg tgagagaaga ccaaaccagt tgcaacttcg
    31801 ccgcgaccaa gtggaccggt tgggccggcg ccgcttctgg tgtagctaag aatgttcttg
    31861 ttaagttctt tcatataagc aatcgcggca tcttgtgagc caaggcgcaa tacctgtgac
    31921 cagagtgcgg tgtatgcagt tcctgaagtt cctgggtggg ccatcatgat gttgcccttg
    31981 tacttaggat ctagaagatc gttccaactt gttggagcgc gaagaccaag acgctttagc
    32041 tcatttgtat ttgaacagaa acctagggct cccatataaa taccggtcca ataaccatcg
    32101 ctgtcttgga actgcttctt gagcatattt ctagttggcg acttgtaagt atctagaact
    32161 ccttgaattc gacctgagat atggctgtca ttcggtccgc cagtccagac atcaaactct
    32221 ggattatttt tggaggcaat tagacgagta agagcttcac ctgatgagag gcgaataaat
    32281 tttgtggtga ttccagtttt tgcttggaat gcctgtgtca tagcaacgca ccaagcctct
    32341 tgtggtgtgc agagaacatt tagcgttcca gagagcttac gtccctgggc atattgcaaa
    32401 gatccgctaa cgcgctcgca tgtgtaggta acaccttgaa tcgtgcgaac taatccctct
    32461 tgtgcacact tacttccctt tagatcacga gctgcttgag ctggagcaat cgctgagaga
    32521 agagagagcg taagagatgc cagagcggcg aaagcaataa ttcttgattt tttcattctt
    32581 acctttcata acttggaagt gatgtgggga ggaattgagg gtgagcgtat cagcgcaggg
    32641 ctaagagttc acaggaggac ttgagagcat tcaggctcat ttcaccaagt ggggcaggta
    32701 gggctcgaac ctacgacgac cggattatga gtccggggct cta
//
LOCUS       DAOROD010000012        29746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_46_length_29746_cov_27.903978, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000012 DAOROD010000000
VERSION     DAOROD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 29746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29746
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_29746_cov_27.903978"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..335
                     /locus_tag="PLW53_03715"
     CDS             18..335
                     /locus_tag="PLW53_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRD36561.1"
                     /translation="MNVRRQIKSTAYGSLLWRVFIGVIGGLVTVIGSALLFAPGPGLL
                     VLLAGLGILATEFAWASRAIKQTKDIAENFSEKIGIPLWVKYLLAALLTLASLLAIAI
                     YYS"
     gene            386..2410
                     /gene="thrS"
                     /locus_tag="PLW53_03720"
     CDS             386..2410
                     /gene="thrS"
                     /locus_tag="PLW53_03720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRD36562.1"
                     /translation="MSPSEIEISVDGVSKKVEAGLKVTQVLEQIYPERMKPGVDAIIV
                     CKINGELKDLWSDISQGDVIESIAITTDEGLNVLRHSTAHVLAQAVQDVFPETKLGIG
                     PPIKDGFYYDFDPKNPFTPDDLIKLESAMKKIVKDGQRFKRRVVTDAEALSELSSEPY
                     KCELIKLKSSDAADGAGVEVGQGELTIYDNLGRDGKALWSDLCRGPHLPSTKYIATYK
                     LMRSAGAYWRGSEKNPMLQRIYGTAWPSQESLDGYLHLLAEAEKRDHRKLGAELDLFS
                     FPEEIGSGLAIFHPKGGAIRMAMEDYSRKRHEEEGYEFVYSPHLTKAALFEKSGHLQW
                     YADGMYPPMIMDEEHHSDGTIKRAGQQYYMKPMNCPFHNLIYQSSAKSYRDLPLRLFE
                     FGTVYRYEKSGVVHGITRARGFTQDDAHIYCSKEQMVDELDSLLTFVLNLLRDYGLSD
                     FYLELSTRNPEKSIGEESDWQDATAALASAAAKQNLELVQDPGGAAFYGPKISVQAKD
                     AIGRTWQMSTIQVDFQLPERFDLGYTASDGSRARPVMIHRALFGSIERFFGVLTEHYA
                     GAFPPWLAPIQVRAIPVAEAFNSYLEKIVADMKKLGIRAEIDASDDRMQKKIRNAQNE
                     KIPFMMIAGEEDVNSGAVSFRYRNGEQKNGIALSDAIAEIQSAISNRKQV"
     gene            2417..2947
                     /locus_tag="PLW53_03725"
     CDS             2417..2947
                     /locus_tag="PLW53_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRD36563.1"
                     /translation="MAFESSGGAGMPDRWERLWAPHRMAYLDGENRPKADNDVACPFC
                     EILSMSDEKALIVARGKEVYAVLNLYPYNAGHMLICSNRHVADLTELSDSERNEMQEF
                     TSHAMKVLRKVSNCGGFNIGLNQGALSGAGIADHIHQHIVPRWFGDANFMPIIGQTKV
                     LPKLLSQTRDELSAAW"
     gene            complement(2950..3591)
                     /locus_tag="PLW53_03730"
     CDS             complement(2950..3591)
                     /locus_tag="PLW53_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36564.1"
                     /translation="MDKPNISPEKSSAMQILLAADASAEPEALAPLLSSDIKVVIIDP
                     KETSVETAQKISEGASAIALLISAKSGISKGMIDLWNYAMDRQFPRMVIVNKLSMSET
                     DFDDIVLIVNRVLEQGVTPYLVLHDELGEPTGLISLESREVHDYSATTPNRYMADSEL
                     QTLVEEFASEYADQLSAFESDSFAHGLLVPILPVMESKLIGVAEIKQYLAQIN"
     gene            3615..4208
                     /locus_tag="PLW53_03735"
     CDS             3615..4208
                     /locus_tag="PLW53_03735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD36565.1"
                     /translation="MIQNRFKAPVTAFITPSCRFLLKIGITANWLTFIGALGSSVSAL
                     YFFSSGKFFLGTILVSLFALSDLFDGTMARLSERGTTKWGALIDSTLDRATDAAIYAG
                     IIAYAISDGDRNLALLALFALITGALIPYIRAKAESLGIACSVGIAERAERLIIILTA
                     TGLYGLGIDLSLVAGLLLINILGIITIAQRLLVVARS"
     gene            4218..5108
                     /locus_tag="PLW53_03740"
     CDS             4218..5108
                     /locus_tag="PLW53_03740"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HRD36566.1"
                     /translation="MIKDWLTYFLYSTLWRFVRTLPEKSAYSLFNSLAKFSYKRNGKR
                     VQRLRRNYSQVRPDASNSELEELVKLGLQSGMRYWCDTFRISDWDKDKILKGVRCIND
                     NFLFEPVASKRGVIVAVPHAGNWDHAGAVFSVRGLKVISVAEHLKPDRLFRRFLKHRE
                     SIGLRILDLDAGVMGELRSFLNQGELVALVADRDLSKSGIDVNFFGARARMPAGPAIL
                     ALETEADLVTVFVSYSQEGLVIDCAPPIKVDKSAERKAEIARVTQVMANRFEDAIRAN
                     PTSWHMQQRIFIDSDFVERA"
     gene            5105..6277
                     /locus_tag="PLW53_03745"
     CDS             5105..6277
                     /locus_tag="PLW53_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD36567.1"
                     /translation="MKIGLISPYAWDVPGGVQAHIRDLAYHYLDQGHQISVLAPASDE
                     SSIVENFVVSAGRPVAIPYNGAVARVLFGPVAASRVRQWVAGGDFDVLHLHEPAIPSL
                     SLLACSIAEGPLVGTFHAAAPRQKVTFAIAPFLEPVIEKLRARIAVSEIARETLRIHL
                     DTDAVVIPNGISKEFYESAEANSAWKREHTIGFIGRFSEPRKGLNILLEALPQIALAI
                     PEVRLLIAGPGEGVEAMESVNPALRSRITFLGRIDDEAKASLLKSISVYVAPNTGGES
                     FGIILTEAMASGVPIVASNIAAFSQLLEDGKYGLLFENENASDLAAKLIRLLKDKELA
                     RKYSELGQGHATRFDWNQVGEEIMNVYLHARGEDEKVTLLSEARPWSRLFTRGEEE"
     gene            6277..6792
                     /locus_tag="PLW53_03750"
     CDS             6277..6792
                     /locus_tag="PLW53_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36568.1"
                     /translation="MEFPVFIIPLIVLFVIWYLSFSATRLDRLHQRVETSWANLDAIL
                     QRRASLALELTHFPETDPAANLLLTSAAHHARAADISVRSEAESALTGVLLLLRQESW
                     LVEKYPDLFEELDQLTERLKVGIALHVEGVSAARARRSKLIYRIFRLAGKAPLPVKYA
                     FEDDSLVEVQR"
     gene            6789..7766
                     /locus_tag="PLW53_03755"
     CDS             6789..7766
                     /locus_tag="PLW53_03755"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRD36569.1"
                     /translation="MRKVAFLALLTLFISSCASLPFAEEKVEIEKNLFTNLPGSSGKV
                     FAVKFDDTNDAHPQQGVESADVVYITQVEAGLTRLMGIYSSNYPEVLGPIRSARISDL
                     SILGEYGRVGFLYSGAQSKMRPVIAESNLINLSAERNPPSIYFNDPSRRSPYSMMVKP
                     NLLLEKAADVELAKKPGWQHGPRAKGAKKIISAEINWPNAGYEAYWSAIDRKFLLKHN
                     SRENIASSGIQLGSPMMVIQMVKISPSEYGDKFGGVTPKSEVIGSGHGYLLRNQRVVK
                     ALWNRASLSEPTTWSLEDGSPAFFAPGQIWFFLTDNEPKFTYLPKPAKT"
     gene            7808..8707
                     /gene="pdxS"
                     /locus_tag="PLW53_03760"
     CDS             7808..8707
                     /gene="pdxS"
                     /locus_tag="PLW53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRD36570.1"
                     /translation="MTKENSTQTGTSRVKRGMAEMLKGGVIMDVVNAEQARIAEDAGA
                     VAVMALERVPADIRAQGGVARMSDPEMITEIQKSVSIPVMAKARIGHFVEAQILQSLG
                     VDYIDESEVLTPADYTHHIDKWNFTIPFVCGATNLGEALRRINEGAAMIRSKGEAGTG
                     DVSNATTHMRAISDEIRRLSSMREDELYVAAKELQAPYDLVKEVATTGKLPVVLFTAG
                     GIATPADAAMMMQLGADGVFVGSGIFKSGDPARRAAAIVKSVTFYDDAKVLAEQSKGL
                     GEAMVGINVADIPAPHRLAERGW"
     gene            8712..9308
                     /gene="pdxT"
                     /locus_tag="PLW53_03765"
     CDS             8712..9308
                     /gene="pdxT"
                     /locus_tag="PLW53_03765"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRD36571.1"
                     /translation="MKIGVLALQGDVAEHIASLGDCGVIASGVRSKQEIEAIDALVIP
                     GGESTTIAKLAKAFGIFDLIKTRIASGMPTYGSCAGMILIADQILDGGVDQESFGGID
                     AQVRRNAFGRQVDSFETDLTFAGISGAAFRAVFIRAPWVESVGTQVEVLASFNGHPVA
                     IRQGHLLATSFHPELTGDNRIHRFFVDQLCKESSKAGI"
     gene            9312..10073
                     /locus_tag="PLW53_03770"
     CDS             9312..10073
                     /locus_tag="PLW53_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRD36572.1"
                     /translation="MSGHSKWATIKRKKALIDSKRSRAFAKYIKAIEVSARNGGADPD
                     GNPTLYDAITKAKKNSVPSDNIERALKRAVGEGQSQAQWENIIYEGYGPGGVAILIEC
                     LSDNRNRAASEVRVAITRNGGTMADPGSVSYMFERKGVVIVKKKQGDKTISEELILDS
                     ALEHGLSEVNDLGESFELISAATDLVPVRQALQNSNIDYESADVSFLPSISIPIGADD
                     ARALFELIEAIEECDDVQNVYGNFDVSDEVMASLS"
     gene            10105..10668
                     /gene="ruvC"
                     /locus_tag="PLW53_03775"
     CDS             10105..10668
                     /gene="ruvC"
                     /locus_tag="PLW53_03775"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRD36573.1"
                     /translation="MRVLGVDPGLTRCGIGVVEKVGVKDLKLVACGVIQTGVDAPLEE
                     RLMHLSAELEIWINRYKPDVIAVERVFSKLNVRTAMATGQAAGVALLLAAKAGVPIAM
                     HTPSEVKAAVSGSGRADKRQVAAMVVRILKLKEAPKPVDTTDALALAICNHWRGAGAK
                     KLATATAKEKSRLKRLKSEPRSRKAKK"
     gene            10669..11265
                     /gene="ruvA"
                     /locus_tag="PLW53_03780"
     CDS             10669..11265
                     /gene="ruvA"
                     /locus_tag="PLW53_03780"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRD36574.1"
                     /translation="MIAFMTGTIFEIRLNRIILLVNGIGYEVIVAPDIASESKVGGEL
                     SLHTSLVVREDSWSLYGFLSQSAKSLFEELQSVTGIGPKVASALLAVYGPDALRNIIA
                     SQDNEALERVPGIGKKVASRILLELKEKFGASYKSKSALSGPWRTQVIGALTGLGYSQ
                     KEAESALDKVLESFGRTPTSEDTPEMLKLALAQSRQKR"
     gene            11269..12306
                     /gene="ruvB"
                     /locus_tag="PLW53_03785"
     CDS             11269..12306
                     /gene="ruvB"
                     /locus_tag="PLW53_03785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRD36575.1"
                     /translation="MSDEVMSSSLTPEEISAEQALRPETLRDFVGQKRVKDQVDLLLR
                     AARERGAPADHILFSGPPGLGKTTLAMIVASEMDSPIRITSGPAITHAGDLASILSSL
                     VEGEVLFLDEIHRMARPAEEMLYMAMEDFRVDVVVGKGPGATAIPLTLPHFTLVGATT
                     RAGLLPGPLRDRFGFTAHLDFYDESDIADIVARSSSLLNVDISKDSIAELGKRSRGTP
                     RIANRLLRRVRDYAQVHGDGSINLEITNSALKMYEVDEIGLDRLDKAILRVLIEQFNG
                     GPVGLGTLAMAVGEERETVESVAEPFLVRSGFIARSPRGRTATPAAWRHLGKKAPEVA
                     MGLFDSGELEP"
     gene            12318..13853
                     /gene="secD"
                     /locus_tag="PLW53_03790"
     CDS             12318..13853
                     /gene="secD"
                     /locus_tag="PLW53_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRD36576.1"
                     /translation="MSYGNSQRNSGEVKKPRPARTLTLTVLTTLILYGVAFAIGATSI
                     NLGLDLRGGTSVTLQPRLQGGQSGEITGESIDQAVSIIRQRVNSLGVVEAEVTAEGTG
                     NSRQIVISVPGETGRRVVDLVGQTAELRFRQVLLVAGSIPTPDDAALLEGSTLSPEQA
                     AAFQALDCTKPESTLGSGGDDANSVMITCNREGSARYILAPAQVVGSDIKGATAAIDQ
                     QSASGWFVSLDFTGDGSRKFGELTQSVVNLAEPQNQVAIVLDGVVVSAPRINEAILGG
                     SAQITGSFSQLEAQDLANVLKYGSLPLAFDRGEVQQISPTLGNDQLQAGLIAGGLGLL
                     LVMIYSIFYYRALGFIVVGSLVIAAAQLVILFLLLGEWIGFTLTLAAVAGAIVAIGIT
                     ADSFIVYFERIRDEMREGKPLRVAAETGWQRARRTIIVADLVSIISAVILYIVSVGSV
                     RGFAFTLGLTTLVDLVVIFLFTKPLVTLLAKNKYFQAGGRYSGVSPRSIGVVTPTLES
                     KGA"
     gene            13857..14873
                     /gene="secF"
                     /locus_tag="PLW53_03795"
     CDS             13857..14873
                     /gene="secF"
                     /locus_tag="PLW53_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRD36577.1"
                     /translation="MKLTGLGGRLYRGETSMNVIRARKKWYAVSALFIALSVFALGAQ
                     GLRFGIEFEGGSAFVVSTSTGTVEQAREAVSAAGYSDNAIIQTLGEEKIRIQTGVLDA
                     AQSNAIQDALAAEFGVSVESIDTQNVGPSWGEEISKKALQGLIAFLICIMIYLALTFE
                     PKMAVAAVVALIHDVFITVGLYAFIGFEVTPATVIGFLTILGYSLYDTVVVFDKIREN
                     TKGITSTGKSTYSDATNLAVNQTFVRSLNTSLIALLPVAAILFVGAGLLGAGTLKDLA
                     LALFIGLIAGTYSSIFIAPPVLASLREREPAMKALSVRVAARGGSTTSPLLSNRKGKK
                     KRNK"
     gene            14894..17065
                     /locus_tag="PLW53_03800"
     CDS             14894..17065
                     /locus_tag="PLW53_03800"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRD36578.1"
                     /translation="MNALITPVVNRLKESQEGSDTSVVERAFEVAERAHKGQLRKSGD
                     PYITHPVAVAEILLDFGLDANSIAAALLHDTVEDTSYSQESLRKDFGPEVASLVDGVT
                     KLDRLTYGPTAEAETVRKMVVAMAKDIRVLVIKLADRLHNARTWQYVEPESAERKARE
                     TLDIYAPLAHRLGMNAVKWELEDLSFKYIEPKKFEEIERLVQERNPSRAKLTNEVIEL
                     VEQDLASESIAAKVTGRHKHLYSVYQKMVIRGREFNDIYDLVGIRVLVDDVRDCYAVL
                     GAIHARWSPVPGRFKDYIAMPKFNLYQSLHTTVIGPNGKAVEIQIRTHDMHSRAEYGI
                     AAHWKYKLSGSSRSSDSPDMLWLKQLHEWQQETSDVTEFLDALRFDLRTPETFVFTPK
                     GSVIALPLGATAVDFAYAVHTDVGNRCIGAKVNGKLVSLEMPLVNGDVVEVLTNKAET
                     ASPSRDWLNFVKSSRARSKIKAWFSKERREEAIDAGRESIARQMRKAGLPLQKILGGH
                     SLLQLAHDLNYPDIEALYAAVGDGHISANHVIERLESSLGLAETYDSPIDENITTASP
                     SRINRRSASGIIVEGVDDVLVKLARCCTPVPGDSITGFITRGNGVSVHRSDCVNVADL
                     KFHQGDRITAVKWDEGAKSVFLVNIQIEALDRARLLSDITRALSDQEINIVHAAVNTT
                     KDQVAFSRFTFEMADASHLEAVLASVKKIEGVYDVYRITNN"
     gene            complement(17062..18366)
                     /locus_tag="PLW53_03805"
     CDS             complement(17062..18366)
                     /locus_tag="PLW53_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRD36579.1"
                     /translation="MDALDNQVLPNSAAAALIGDPSKFGRVDENGMVYVRTSDGEKVV
                     GSYPGKSAEEALAYFVRKFELVASEVALLAARIISGAMVPQDANEAVKKLRNQIEHLN
                     GVGNLEALRSSLEQIPPLIDEHRPAYEAKKAAEAEAKAEKRAATIAAKEALVVEAEGL
                     IDSTSWKVTGERLKVLLDEWKKAPRLDKKTDSELWKRFSSARNKFDKRRRVHFAGLSA
                     QQSQVKDAKEKIVAEAESLATSTDWVATARRYKTLMDQWKASGRGKKSDDDKLWTRFK
                     AAQDTFFTSKNADLEKRGESMAANLEKREAIVAEMEALLPITNLDDVRRKFRDLRQKL
                     SKIGMIDRKKRSVLDKRVDSVEMTIKEAEQEQWRRSDPTAIARANDVVTQLTNAVAEY
                     EAKAAKAQANGDSKKAADLLEAAAARRMWLSEAQKGLADFQS"
     gene            18537..19244
                     /locus_tag="PLW53_03810"
     CDS             18537..19244
                     /locus_tag="PLW53_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD36580.1"
                     /translation="MSLLVDRVVAPYYATNCWIIAPAKNSHCVVIDPGIAIPNLMKEI
                     KAKLEEHNLVVGAIFITHGHLDHTFSLYSLAKDFIESDTYIHKADRDLLSNPERAMGP
                     QSQALLSELGKLSGEGINFQEPERTWSIDSDSESKLGGMNFSFRHAPGHTPGSTLAIV
                     DDELLISGDVLFKGGIGRTDLPRGSMSDMEITLREKIATLPPHLRVLTGHGDESRMEE
                     ELKSNQYLIAATQGRLA"
     gene            19241..20521
                     /gene="hisS"
                     /locus_tag="PLW53_03815"
     CDS             19241..20521
                     /gene="hisS"
                     /locus_tag="PLW53_03815"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRD36581.1"
                     /translation="MSQKFAAPKGVSEYLPPKSNVFDRIIQTLKVPALAAGYELIELP
                     IFEDTELFTRGVGASTDVVTKEMYTFEDRGGRSLTLRPEGTAGVIRAVIEHGLDRGSL
                     PVKLFYHGPFFRAERPQAGRYRQFYQFGIEAIGSNDPYLDAEVISIAYQGYRKLGLKD
                     FELQITSLGDSESRATHRKDLDAFIAGLNLDEETRARALKNPLRLFDDKREEIRASMN
                     KAPLLLDYLNSESRENFEAVKSALDSLEISYVVNPRMVRGLDYYTGTTFEFVHNGLGA
                     QSGIGGGGRYDGLMELLGGSKLSGIGFGLGIDRILLALEAEAKSGQLLPGELKYIDLY
                     VIALGDEARSKCFTLVDSLRKEGFVVEQNFGDRSLKGAMKAADKSGARFAIVLGDSEL
                     QSGSVELKQMSNGEQTSVRLTSLAKTLAEKSSGL"
     gene            20547..22280
                     /gene="aspS"
                     /locus_tag="PLW53_03820"
     CDS             20547..22280
                     /gene="aspS"
                     /locus_tag="PLW53_03820"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRD36582.1"
                     /translation="MLRSHSAGTLRSSDIGSRVKLAGWVARRRDHGGVAFIDLRDYSG
                     SVQVVISDEKVAAALRAEWCVLIDGVVTKRPSGNENSALPTGEIEVNCESLEVLSEAA
                     ALPFPVDSGEVVNVSEEVRLKYRYLDLRREGPSSALRLRSKVTSIIRSVMDSEGFLDI
                     ETPNLTRSTPEGARDFLVPVRLQPGSWYALPQSPQLFKQLLMVAGMERYYQIARCFRD
                     EDFRADRQPEFTQLDIEMSFVDQSDVIAVAEKILKEVFKRTLDIDISLPIAHMTYHEA
                     LRRFGSDKPDLRFENELVDVTDFFKETSFRVFQAEFVGAVVMPKGAASPRRELDAWQD
                     WAKARGAKGLAYILVGEDGSLGGPVAKNLSEKESKEIASFVGAKNGDAIFFAAGDRSS
                     SLQLLGAVRLEIGQRCNLIAEDQWKFLWVVDAPMFEATDSGGWTAVHHPFTGPKPEYE
                     KSFKDDPKSALAYAYDIVLNGNEIGGGSIRIHKREIQQAVFDVIGLTKQEAESKFGFL
                     LEAFNYGPPPHGGIALGLDRLCALLAGAESIREVIAFPKTASGADPLTGAPTPITTAQ
                     RRESGVDFKPE"
     gene            22309..22707
                     /locus_tag="PLW53_03825"
     CDS             22309..22707
                     /locus_tag="PLW53_03825"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRD36583.1"
                     /translation="MSAGGIAAIIAASALLIIGIAIAYAVIRLGRAIDEVGGAVRDIA
                     KETAPLLNEVTTTVELVNGPLQSINKVTKTMEEFSSKVSESASGFLDKNQMAMKAAGA
                     VLTAAQLRKKKKGKKSKKAKNSRYVDDEEF"
     gene            22714..25389
                     /gene="alaS"
                     /locus_tag="PLW53_03830"
     CDS             22714..25389
                     /gene="alaS"
                     /locus_tag="PLW53_03830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRD36584.1"
                     /translation="MNSAEIRSRWLKFFESANSQGIKHTVVPSASLIADDPNLLLVNA
                     GMVPFKPFFLGEITPPYKRATSVQKCVRTLDIEEVGKTTRHASFFQMCGNFSFGDYFK
                     EGAITLAWELLTKPISDGGYGFAEDKLWVTVYLDDDEAADIWHKRIGIPKERIQRRGM
                     ADNFWSMGVPGPCGPCSEIYFDRGAAYGKEGGPIADEDRYLEIWNLVFMQNIRGEGGG
                     KESFPILGELPAKNIDTGLGLERTAALLQGVENIYEIDTTQHILNRASDLSGVTYGKA
                     TSSDVSLRVIADHARTATMLIGDGVTPGNEGRGYVLRRMMRRTTRNMKLLGADDRNMS
                     ELVKASIEAMGPQYPELQSDSGRILKVAEAEEESFLQTLKSGTLIFDQRSASLKKSGA
                     KVLSGEDVFQLHDTYGFPFDLTLEMANEEGLSIDAEGFTRLMKQQRERAKADAKSKKS
                     GHTDVTEYRKIAESAGKLEFVGYESQESEAKIVGFLLDGAGVKSASAGDDVEIVLDRT
                     PFYAEGGGQLADSGVITTSDGSVIEVEDVQAPIPGVFIHRGRVLSGQLEIGESVLAKI
                     DSDRRKAISRAHTATHMVHKAFREILGETATQAGSENAPGRFRFDFPSSGAVSEGVLN
                     DVEARVNSLLLENLLVSAEEMSQAEAKKIGAMALFGEKYGERVRVISVGDWAKELCGG
                     THVSRSGELGIVKLLGESSIGAGVRRVEALVGADAYSFLSREHILINSLLEIIKATRP
                     EELPERISSLLARLKEAEKEIGSIRTAKALSDSAALVKNAEDIGQFKLISADMGKGLS
                     GDDLRTVALDLRNRLKGVVVALLSSGDEKVTLVVATDETARSAGAKAGALVKIASGIL
                     GGGGGGKDDFAQGGGSDFGKIDEALKAIKAALSSI"
     gene            25398..25850
                     /gene="ruvX"
                     /locus_tag="PLW53_03835"
     CDS             25398..25850
                     /gene="ruvX"
                     /locus_tag="PLW53_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRD36585.1"
                     /translation="MIQGARLAIDYGERRIGIAKSDNSRLIASPVVTLDNSESTGNVY
                     SAIKDIVSESNAMLVYIGLPLHLSGKESASSEKARVFAGLLKQHLPQEVQIRLLDERL
                     TTSSAVGELKNAGLKSSRESIDQLAAVKILEFALEVERLSGKAAGHEV"
     gene            25840..26892
                     /gene="mltG"
                     /locus_tag="PLW53_03840"
     CDS             25840..26892
                     /gene="mltG"
                     /locus_tag="PLW53_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRD36586.1"
                     /translation="MKFRAASIKQSLESSPLLRLATAGIFLLLLTLSLRELNSSPQSA
                     PDFEAGSAGEEVIVEILSGESGSEIGKKLELLSVVKSSTAFFRVAVADPRSQRIAPGE
                     HRIETRIPARTALEQLLDPSRIPNLIVVRDGQRLSEISESIINFGISKAELEKSIKAF
                     SPPKIFKSKNIEGFLYPAQYSFNKSVDADEIVSAMLKRFSSATKDLNWKNERELTPFE
                     VMVIASLVQTEGTPDVFGKVSRVIYNRLEKRMPLQFDSTVHYALGRRGEIRVSLNDIK
                     VKNRYNTFIYPGLPPGPIGSPTKAAIDAALNPEAGNWLYFVTVKPFDTRFTNSYDQFL
                     GWKSEYKRNFKAGLFE"
     gene            26889..27698
                     /locus_tag="PLW53_03845"
     CDS             26889..27698
                     /locus_tag="PLW53_03845"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36587.1"
                     /translation="MMKRAAVLGSPISHSLSPLIHNHAYSLLEYPAIYEAIEVKSGEL
                     SEFLTRELKSSDVLGFSLTMPLKEELAQLSIPALQVEARAKAINSANTIYKDGQSWKV
                     SSTDVDGFEFLLKELSFSTVSILGAGGTARAAIASLSDRDVKIEIYRRSTSRDASLSE
                     AAMSSKIEIYDWDKCARAWKSDLVINTIPSDQVLSIIKGFSSPKILLDATYSPWPPGL
                     TKQQLDAKGLLVSGLQLLAAQAVYQISLMTQIDFDKTWLYNELILKLVSAQ"
     gene            28230..29411
                     /gene="aroC"
                     /locus_tag="PLW53_03850"
     CDS             28230..29411
                     /gene="aroC"
                     /locus_tag="PLW53_03850"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRD36588.1"
                     /translation="MRWLTAGESHGPALVGILEGMPASVSVTTADIDEDLRRRRLGFG
                     RGARQNFEADKVTILGGVRHGLTQGGPIAIQIANSEWPKWEKVMSADPVDEAEIESLG
                     RNAPLTRPRPGHADLVGIQKYDFDDARPVLERASARETAARVALGAVARRFLKQVGGI
                     EVFSHVVSIGAVSVADDYLLPEPKDRERIDSDEVRSAFKDVSAKMVEEINDAHKNGDT
                     LGGVVEVLVYGLPPGLGSHVHWDRRLDSRLAGALMGIQAIKGVELGDGFTTARKRGSS
                     AHDEIERDNLGNIRRRTSRSGGTEGGMSTGEVLRVRAAMKPISTVPKALDTIDITNGQ
                     AAKAINQRSDVCAVPAAGIVAEAMVALVLAEAMLEKFGGDSLGESKRNLENFLSSMKV
                     K"
     gene            29415..>29746
                     /locus_tag="PLW53_03855"
     CDS             29415..>29746
                     /locus_tag="PLW53_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRD36589.1"
                     /translation="MPPIILIGPPGAGKTSVGKALAKKLSLNFLDSDKVVEEKSGKSI
                     SEIFITDGEPAFREMERAAVIDLIENQDGVIALGGGSVMDLEVSKRLLPMANVVFLDV
                     SISNAAPRV"
BASE COUNT         8225 a         6057 c         7301 g         8163 t
ORIGIN      
        1 ttgggtaatc tagagcagtg aatgtaagac gccagattaa gtccacggcc tatggctcat
       61 tgttatggcg cgttttcatt ggagtcattg gtggattagt aacggtgatt ggttcagctc
      121 ttctctttgc gccaggtcct ggacttcttg ttcttcttgc tggtcttggt attttggcaa
      181 cagagtttgc ttgggcatca agggcaatca aacagactaa agatattgca gagaattttt
      241 cagagaagat tggaatcccg ctctgggtta aatatctcct cgctgcactt cttaccctgg
      301 catctctgct tgcaatcgcg atttattaca gctgataacc gatctcttaa ggcgatagag
      361 gcccaggctt tggtagcctg atgtcatgtc gccaagtgaa attgaaataa gtgttgatgg
      421 cgtctccaaa aaagtagaag cagggctcaa ggtcacccaa gtcctggagc agatttatcc
      481 agagagaatg aagcctggag tagatgcgat catcgtatgc aaaataaatg gagaactaaa
      541 agatctttgg agcgatatat cgcaaggtga tgtaattgaa tcgatagcaa ttactacaga
      601 tgagggactt aatgtcttgc gccactccac agcgcacgtt ctggcgcaag cggtgcagga
      661 tgttttcccc gaaactaaat taggtatcgg ccctccgatt aaagatggct tctattatga
      721 ctttgatcca aagaacccct ttactccaga tgatctaatc aaacttgaat ctgcgatgaa
      781 gaagatagtt aaagatggcc agaggtttaa acgaagagtt gttactgacg cagaggcgtt
      841 aagtgagtta tctagtgagc catataaatg cgagctgatt aaattgaaat catcagatgc
      901 tgcagatgga gctggcgttg aagtcggcca aggcgagctt acgatctatg acaatttagg
      961 aagagatgga aaggcgctct ggagtgatct ttgtagaggt ccgcatctac cctcaactaa
     1021 atacatcgct acctataaat taatgagaag cgcaggagca tattggcgcg gctctgagaa
     1081 aaatccgatg ctgcagcgaa tctatggcac cgcttggcca tcgcaggaat cactcgatgg
     1141 ctacctacat cttctagctg aggcagagaa gcgcgatcat agaaaacttg gcgcagagtt
     1201 agatctcttc tcatttcctg aggagattgg ctctggtctt gcaatctttc atcctaaagg
     1261 tggagctatt cgaatggcga tggaggatta ctctagaaag cgccatgaag aagagggata
     1321 tgagtttgtc tactcaccac acttaactaa agctgcgctc tttgaaaagt cgggccatct
     1381 tcaatggtat gccgatggaa tgtatccgcc aatgattatg gatgaagaac accatagcga
     1441 tggaaccatt aaaagagctg gccagcagta ttacatgaag ccgatgaact gcccgttcca
     1501 caatttgatt tatcagtcca gcgctaagtc ttatcgcgat ctaccacttc gattatttga
     1561 gtttggaact gtatatcgat atgaaaaatc tggcgtggtt catggcataa caagagcgcg
     1621 aggctttact caagatgatg cccatatcta ttgcagtaaa gagcagatgg ttgatgagct
     1681 agatagcctc ttaacgtttg tgttaaatct tttgcgtgat tatggtttaa gtgatttcta
     1741 tctagagctt tccactagaa accctgagaa atcaattggc gaggaaagtg attggcaaga
     1801 tgcaactgca gcccttgcta gcgctgctgc aaagcagaat ttagagttag tccaagatcc
     1861 tggcggggca gccttttatg gtccaaagat ttcggttcaa gcaaaagatg ccatcggtag
     1921 aacttggcag atgtcgacga ttcaagtgga tttccaactc ccagagcgtt ttgatcttgg
     1981 ctacaccgca tcagatggat ctcgcgctcg accagtaatg attcatagag cgctctttgg
     2041 ttcaatcgag cgtttctttg gagtattaac cgagcactac gcaggcgcat ttcctccctg
     2101 gcttgcgcca attcaagtca gggccattcc ggtagcagag gcctttaatt cctatcttga
     2161 gaagattgtt gcagatatga aaaaacttgg aattagagct gaaattgatg catcagatga
     2221 tcgaatgcag aaaaaaatta gaaacgcgca gaacgaaaag attccattca tgatgattgc
     2281 tggagaagag gatgttaact ctggagcggt ttccttccgc tatcgaaatg gagaacagaa
     2341 aaacggaatt gccctctctg atgcaattgc tgaaattcaa tcggcgataa gtaatcgaaa
     2401 gcaggtttag ttcttcatgg cttttgaatc aagcggggga gcaggcatgc ctgatcgctg
     2461 ggagcgcctt tgggcccctc atcgcatggc atacctggat ggagaaaata ggccgaaagc
     2521 tgataatgat gtggcctgcc ctttttgtga aattttaagt atgagcgatg agaaggctct
     2581 gattgtggca agaggtaaag aggtctatgc cgtattaaat ctctatcctt ataacgcagg
     2641 ccacatgctt atctgcagta atcgccatgt cgcagattta acagagctaa gcgatagcga
     2701 acgcaatgag atgcaagaat tcacttctca tgccatgaaa gttcttcgca aagtttcaaa
     2761 ttgtggggga tttaatattg gcttaaatca aggcgcttta tctggcgctg gaattgctga
     2821 tcacatccat caacatattg ttccgcgctg gtttggtgat gctaatttca tgcccataat
     2881 tgggcagacc aaagtacttc caaaattact tagccagact agagatgagc tctcagctgc
     2941 ttggtagctc taattgattt gagctagata ctgcttaatt tcagcgactc caatcaactt
     3001 ggattccatc actggaagta ttggaaccaa aagtccgtga gcaaaagagt cagattcaaa
     3061 agctgatagt tgatctgcat attcagaggc aaactcctcg accagggttt gaagctcgct
     3121 atctgccata tatctatttg gggtagtagc tgaatagtcg tgcacttctc tgctctctag
     3181 tgaaattaaa ccagtcggct ctccgagttc atcatgtaag actaaatatg gcgttactcc
     3241 ttgttcaaga acgcgattga ctataagaac gatgtcgtca aagtctgtct cactcatact
     3301 tagtttattt acgattacca tccttggaaa ttgtctatcc attgcatagt tccagagatc
     3361 aatcatccct ttggagattc cgctcttagc acttatcagc agggctattg cgctagctcc
     3421 ttcagatatt ttctgagcag tctcaacgct cgtctctttg gggtcaataa ttacaacctt
     3481 aatatcactc gagagtaggg gggcaagggc ttctggctca gcggaagcat ctgctgctag
     3541 cagaatctgc atggcgctac ttttctcagg cgagatattg ggtttgtcca tggctaaagc
     3601 ctacgatgta ctcaatgatt cagaatcgat ttaaagcgcc agtcacagcc tttattaccc
     3661 ctagctgtag gttcttacta aagattggga taactgctaa ctggctgact tttattggag
     3721 ccttaggcag ttctgttagc gctctatatt ttttctcctc tggaaagttt tttcttggaa
     3781 ccattctggt ttcactattt gctctaagtg atctcttcga tggaacgatg gctaggctat
     3841 ctgagagggg aacaactaag tggggcgcgt taattgattc aactctagat cgcgcaactg
     3901 atgccgccat ctatgctgga atcattgctt atgccatttc agatggcgat aggaatttag
     3961 ctctcctggc actctttgca ctgattaccg gagccttgat tccctacatc agagcgaagg
     4021 cggaatcact tggaattgct tgcagcgttg gaatcgcaga gcgagcagag cgcttaatta
     4081 tcattttgac cgcaactggc ctctatggtc ttggcattga cctttctttg gtggcgggat
     4141 tactacttat aaatatctta gggataatta ccatagcgca gcgcttatta gttgtagctc
     4201 gcagctagga agaagtgatg attaaggatt ggttaaccta tttcctttat agcacccttt
     4261 ggagatttgt tagaactctg cccgagaaaa gtgcttactc actctttaat tctcttgcca
     4321 agttttcata caaaagaaat ggcaaaagag tgcaaaggtt gcgccgcaac tactcgcaag
     4381 taagaccaga tgcgagcaac tctgagttag aagagttagt taaattggga cttcaatctg
     4441 ggatgagata ttggtgcgat accttcagaa tttctgattg ggacaaagat aagattctaa
     4501 aaggcgttag gtgtataaac gataattttc tctttgaacc cgttgctagt aagcgcggag
     4561 tgatagttgc agttccccat gctggtaatt gggatcatgc tggcgcagtc ttctcggttc
     4621 gtggtttaaa ggtcatcagc gttgctgaac atctaaagcc agatcgtctc tttagaaggt
     4681 ttctaaagca ccgtgaatca atcggtcttc gaattctgga tctcgatgct ggagttatgg
     4741 gcgagcttcg ctcatttctc aaccagggag aattggttgc cttagttgca gatagagatt
     4801 taagtaagtc aggaattgat gtcaatttct ttggtgccag agcaaggatg ccagctggtc
     4861 cagcaattct agctcttgaa acagaagcag atttagtcac tgtctttgtc tcctatagtc
     4921 aggaagggtt agttatagat tgtgctccgc caataaaggt tgataaaagc gcagagcgaa
     4981 aggcagagat tgctcgcgta actcaagtaa tggcaaatag attcgaggat gcgataagag
     5041 caaatccaac ttcctggcac atgcaacagc gcatcttcat tgatagcgat ttcgtggagc
     5101 gagcatgaaa atcggattaa tttcgcctta tgcttgggat gttccaggtg gtgtgcaagc
     5161 tcatattaga gaccttgctt atcattatct tgatcaggga catcagatat ctgttttagc
     5221 tccggcaagt gatgagagtt caatagtaga aaactttgtg gtcagtgcag gtagacccgt
     5281 tgctattcct tataacggcg cagttgcaag agttctcttt ggtccagttg ctgcatcaag
     5341 agttaggcag tgggtggctg gaggagattt tgatgttctt catcttcacg aaccagcaat
     5401 tccatcccta tctcttcttg cctgttcaat tgctgaagga ccacttgttg gaacatttca
     5461 tgccgcagcc ccgaggcaga aggtcacctt tgccattgcg ccatttttag aacctgtcat
     5521 tgagaagtta agagccagaa ttgctgttag tgaaatcgca agagaaacac ttcgaattca
     5581 cctagatact gatgccgtag taattcccaa tggaattagc aaggagttct acgaaagcgc
     5641 agaagcaaat tcagcttgga aacgggagca caccattggg tttattggca gattttcaga
     5701 gccgcgaaag ggactaaaca ttctccttga ggcccttccg caaattgctc ttgccattcc
     5761 ggaggtgcgc ttgctgattg caggcccagg agaaggtgtt gaagccatgg agagcgttaa
     5821 tccagctctt cgttctcgaa tcacatttct tggcagaatt gatgatgagg ccaaagcatc
     5881 tctccttaaa tcaattagcg tctatgtagc cccaaatacc ggcggagaat cttttggcat
     5941 aatcctcact gaagcgatgg catctggcgt gccgattgtg gcgagtaaca ttgcagcctt
     6001 tagtcaactt cttgaggatg gtaaatacgg acttcttttt gaaaatgaga atgcttctga
     6061 tcttgctgcg aaattaattc gccttctcaa agataaggaa ttagcaagaa agtattctga
     6121 acttggccaa ggccatgcca ctagatttga ttggaatcag gttggcgaag agataatgaa
     6181 tgtttatctt cacgctagag gcgaagatga gaaggtgacg cttttgtctg aggcaaggcc
     6241 atggagcagg ctctttacta gaggggagga ggagtaatgg agtttccagt atttataatt
     6301 cctctaattg ttttatttgt tatttggtat ctcagtttta gcgcaacaag gcttgatcgt
     6361 cttcaccaga gagtagaaac atcctgggca aatcttgatg caattttgca gcgacgagcc
     6421 tcactggcac ttgaactaac ccattttcca gagaccgacc cagctgcaaa tcttctactc
     6481 acctcagctg cccatcatgc aagggctgct gatatatctg ttcgcagtga ggcagagagc
     6541 gcacttaccg gagttctatt gcttcttagg caggagagct ggttagttga gaagtatcct
     6601 gatttattcg aagaacttga tcaattaact gagcggctca aggttggaat tgctctacat
     6661 gtagagggag tttcagctgc gagagcacga cgatctaagt tgatttatcg aatctttcgt
     6721 cttgctggaa aagccccact tccagtcaaa tatgccttcg aagatgattc cttagttgaa
     6781 gtacaaagat gagaaaagta gcctttcttg cacttctcac tctatttata tcatcctgcg
     6841 catcgcttcc ttttgcagaa gagaaggtag agattgaaaa aaatctcttc acaaatcttc
     6901 ctggaagcag tggaaaagtt tttgcagtca agttcgatga cacaaatgat gcacatccgc
     6961 agcagggtgt ggagtcagct gatgttgttt atataacgca agtcgaagca ggtttaacaa
     7021 ggcttatggg aatctactcg tcaaattatc cagaggtctt agggcctatt agatctgccc
     7081 gaatcagtga tctatcaatc cttggcgagt atggcagagt tggattcctc tatagtggcg
     7141 cgcaaagcaa gatgcgccca gttattgcag agtcaaattt aatcaaccta agcgctgaac
     7201 gaaatccacc ctcaatatat tttaatgatc catcacgtcg ttctccttat tccatgatgg
     7261 tgaagccaaa tctactgctt gaaaaagctg cggatgtaga attggcaaag aaaccaggat
     7321 ggcaacatgg ccctagggca aaaggcgcta agaagataat ttctgccgag atcaattggc
     7381 caaatgcagg ctatgaggct tattggagcg caatcgatag aaaatttctt ttaaaacata
     7441 atagtcgaga aaatattgcc tcatctggca ttcagctagg ttcaccaatg atggtgattc
     7501 aaatggtgaa aatctcgcca tcagaatacg gcgataaatt tggcggagta acgccaaaga
     7561 gcgaagtaat tggaagtggg catggatacc tactgcgaaa ccaaagagtt gttaaagctc
     7621 tatggaatag agcgagcttg agtgaaccaa ctacttggag cctcgaagat ggcagccctg
     7681 cattttttgc gccaggacag atctggttct ttttaaccga taatgagccc aaattcacct
     7741 atctgccgaa gccagccaag acctagtccg attcgccaga tggcctaatc aaagtagaat
     7801 cacgcctatg acaaaagaga attcaaccca gaccggaaca tcgcgcgtta aacgcggcat
     7861 ggcagagatg cttaaaggcg gggtaattat ggatgtcgtt aatgctgagc aggcgcggat
     7921 cgcagaagat gctggagccg ttgcagttat ggcacttgag cgagttcctg cagatataag
     7981 agctcaaggc ggagtggctc gcatgtcaga tccagagatg attacagaga ttcaaaagag
     8041 cgtatcaatt ccggttatgg ctaaagctcg tatcggtcat ttcgtcgagg ctcagatttt
     8101 gcaatcactt ggggttgatt acattgatga atcagaggtg ttaactcctg ctgattacac
     8161 ccaccatatt gataaatgga atttcacaat tcctttcgtc tgtggggcaa ctaatcttgg
     8221 tgaagcctta agaagaatta atgaaggcgc ggcgatgatt cgttctaaag gtgaggcagg
     8281 aactggcgat gtttcaaatg ccaccactca tatgcgcgct atctcagatg aaattcgtag
     8341 attaagttca atgcgcgaag atgaactata tgttgcagca aaggagcttc aagctcccta
     8401 tgatttggtg aaagaagttg ctacaactgg aaaacttccc gttgttctct ttaccgcagg
     8461 tggaattgct actcctgctg atgctgccat gatgatgcag cttggagcag atggagtatt
     8521 tgttggctca ggaatattta aatcaggcga tccagcaaga agagcagccg ccattgttaa
     8581 atcagtaact ttctatgatg atgcaaaagt tctcgctgag caatcaaaag gtcttggtga
     8641 agcgatggtt ggaattaacg ttgcagacat cccagcccca catcgtttag cagagcgagg
     8701 ctggtaggaa agtgaagatt ggagtcttag cgctccaagg tgatgttgcc gagcatattg
     8761 cctcacttgg agattgcggc gttattgcaa gcggggtcag aagtaagcaa gaaatcgagg
     8821 cgatagatgc cctggtaatt ccaggaggag aatcgacgac aattgccaaa cttgcaaaag
     8881 catttggaat ctttgacctt attaaaactc gtatcgcatc tggaatgcct acctacggct
     8941 cctgcgctgg aatgatttta attgccgatc aaatacttga tggtggagtc gatcaagaga
     9001 gtttcggtgg cattgatgcc caagttcgta gaaatgcatt tggtagacag gtagattcct
     9061 ttgaaactga tctaactttt gcaggcataa gcggagctgc tttccgcgca gtttttatca
     9121 gagctccttg ggttgaatcc gttggaactc aagtagaagt tttagcaagt tttaacggcc
     9181 atcctgtcgc tatcaggcag gggcacttac tagctacctc gtttcatcca gagttaactg
     9241 gtgataatcg catccatcga ttctttgtag accagctctg taaagagagc tcaaaggcag
     9301 ggatctagaa aatgtcaggt cattccaaat gggccacaat caaacgcaag aaagctttga
     9361 tagattctaa gcgatcaagg gcatttgcta aatacattaa agcaattgag gtatctgcaa
     9421 gaaatggtgg agcagatcca gatggtaatc ccactttgta tgacgcaatt actaaggcca
     9481 agaagaattc agttccaagc gataacatcg aacgagctct aaagagagca gttggcgaag
     9541 gtcaatcaca agctcaatgg gagaacataa tttatgaagg ttatggtcct ggcggggtag
     9601 caattcttat cgaatgctta tcggacaata gaaatagagc ggcatcagag gttcgagtcg
     9661 ctatcactag aaatggtggc actatggccg atcctggttc agtttcctat atgttcgagc
     9721 gaaaaggcgt agtaattgtt aagaagaaac aaggtgataa aactataagc gaggagttga
     9781 tcttagattc ggccttggag catggtttat ccgaggttaa tgatttaggc gagagctttg
     9841 aacttattag cgctgcaaca gatttggttc cagttagaca ggctctgcaa aattcaaata
     9901 ttgattatga atcagcagat gtttccttcc ttccttctat ttctattcca attggcgcag
     9961 atgatgccag agccctcttt gagttaattg aggccataga ggagtgcgat gatgtgcaga
    10021 atgtctatgg aaactttgat gtttcagatg aggtaatggc gagcctgtca taacactttc
    10081 ttgactggct aataggcttg agccatgcgc gttcttggtg ttgatcccgg tcttacgcgc
    10141 tgcggtattg gagtagttga aaaagttgga gtaaaggatc tcaagttagt tgcctgcggg
    10201 gtaattcaaa ctggcgttga tgctcctctt gaggaacgtt tgatgcatct aagtgccgag
    10261 cttgagatct ggattaaccg atataagcca gatgtaattg cagtggagcg agtcttttca
    10321 aagctcaatg tgcgaaccgc aatggctaca gggcaagcag ctggagttgc gctacttctt
    10381 gcagcaaagg caggagttcc aattgcgatg cacaccccaa gtgaagtcaa ggcagctgtc
    10441 tctggttctg gacgagccga taagaggcag gtagctgcca tggtagttag aatcttaaaa
    10501 ttgaaagaag cgccgaaacc agtagatacc accgatgcgt tagcccttgc tatctgcaat
    10561 cattggcgag gagctggagc aaaaaaattg gcaactgcca cagctaaaga aaaaagccgt
    10621 ttaaaacgtt taaagtcgga gccaagatca agaaaggcga agaaatagat gattgccttc
    10681 atgactggca ctatttttga gatccgactt aaccgaatca ttcttcttgt taatggaatt
    10741 ggttatgaag ttattgttgc tccagatatc gcttctgaat caaaagtagg aggcgagctc
    10801 tcgcttcaca catcgttagt tgtaagagag gattcctgga gtctttatgg attcttatct
    10861 cagagcgcaa agagcctttt tgaagaactg caatcagtaa ccggaatcgg accaaaagtt
    10921 gctagcgctc tgcttgcagt ttatggccca gatgcgctgc gaaatattat tgcctcacaa
    10981 gataatgaag ctctggagcg agttcctgga attgggaaga aagttgcttc aaggattctt
    11041 ctagagttaa aagagaagtt tggcgcaagc tacaaatcaa aatctgctct aagcggtcca
    11101 tggcgaactc aggtcattgg cgcactaact ggacttggtt attcccaaaa agaggcagaa
    11161 agtgcgctag ataaagtact ggaaagcttt ggaagaactc cgacaagtga agatactccc
    11221 gagatgctaa agctagcgct ggcgcaatcg cgacagaaga gatagctcat gagtgatgaa
    11281 gtaatgagta gttcacttac acccgaggag ataagcgcag agcaggcctt aaggccagaa
    11341 acactaagag attttgtagg acaaaagcgc gttaaggatc aagtcgatct gctgcttcga
    11401 gcagctagag agcgcggcgc tccagcagat cacattctct tctcaggacc tcctggtctt
    11461 ggcaaaacta ctcttgcaat gattgtggca agtgagatgg attcaccaat aagaattacc
    11521 agcgggccag caattaccca tgctggcgat cttgcttcaa tactttcatc acttgtcgag
    11581 ggagaagttt tatttctcga tgagattcat agaatggcaa ggccagcaga agagatgctc
    11641 tatatggcga tggaggattt tagagttgat gttgtggttg gcaaaggacc aggggcgact
    11701 gcaattccat taactttgcc acactttact ttagttggcg cgacaactag agcaggactt
    11761 ctgccaggac cgctaagaga tcgctttggt tttactgctc atctagattt ctatgatgaa
    11821 tcagatattg cagatattgt tgctcgaagt tcatcgcttt taaatgttga tattagtaaa
    11881 gattcaattg cagaattagg aaagcgctca cgaggaacac cgcgtattgc aaataggctg
    11941 cttcgtagag ttagggatta cgcccaagtt catggagatg gaagtatcaa tctcgagata
    12001 actaactctg ctcttaaaat gtatgaagtt gatgagattg gtcttgatag attagataaa
    12061 gcaattctta gagtattgat agaacagttc aatggcggac ctgttggtct tggcacactt
    12121 gcgatggccg ttggtgagga gcgagagact gttgaatctg tagccgaacc atttctggtc
    12181 cgctcaggat ttatcgcaag atcgccgagg ggaagaacgg ctacgccagc agcttggcgc
    12241 catctaggca agaaagctcc tgaggtggcg atggggcttt tcgacagtgg ggagcttgag
    12301 ccctagactt tcgccccatg tcttacggaa attcacaacg taactctggc gaggtcaaga
    12361 aacctcgccc agctagaacc ttaactctta cagttttaac cactcttatt ctctacggcg
    12421 ttgcctttgc aatcggtgct acctcaatca acttaggtct agatctaaga ggcggaacga
    12481 gtgtgacctt gcaaccaaga ctgcaaggtg ggcagagcgg cgagataaca ggtgaatcaa
    12541 ttgatcaagc tgtatcaatt atccgtcagc gcgttaactc acttggcgtt gttgaagccg
    12601 aggtaactgc agagggaact ggcaatagca gacagattgt tatctccgtt ccaggtgaaa
    12661 ctggtagacg agttgtggac cttgttggtc aaactgcaga acttagattt agacaggtat
    12721 tacttgttgc aggatcaatt ccaactcctg acgatgccgc tttgctagaa ggatcaactc
    12781 ttagcccaga acaagccgca gcatttcaag cacttgattg caccaaacca gagtcaactc
    12841 ttggtagcgg gggagatgat gctaattctg taatgatcac atgcaataga gaaggctctg
    12901 caagatatat tcttgctcca gctcaagttg ttggaagcga tattaaaggc gcaactgctg
    12961 ccattgatca acagagcgca agtggttggt ttgtctcact tgatttcact ggagatggat
    13021 ctcgtaagtt tggcgaacta actcagagcg ttgttaatct tgccgagcct caaaatcaag
    13081 tagcaatcgt tcttgatggc gtggttgtct ctgctcctag aatcaatgag gcgatcttgg
    13141 gcggcagcgc gcagattact ggcagcttta gccaactcga agcgcaagat cttgccaatg
    13201 ttttgaagta tggctcacta ccccttgcct ttgatcgcgg agaagttcag caaatctcgc
    13261 caactcttgg aaacgatcag ttgcaggcgg gtcttattgc tggtggcctt ggattacttc
    13321 ttgtgatgat ttattcaata ttttattatc gcgctctagg ttttattgtc gtcggatctt
    13381 tagttattgc cgccgctcaa ctggtaattc tcttcctgct cttaggtgag tggattggat
    13441 ttactctaac tcttgcagct gttgctggag caattgtggc aattggaatt acagctgact
    13501 catttattgt ctactttgaa cgtattagag atgaaatgcg agaaggaaaa ccactacgcg
    13561 ttgctgccga gactgggtgg caaagagcac gtcgaacaat catcgttgca gacctagtat
    13621 caattatttc agcggtaatt ctctatatcg tctcggttgg aagcgtccgt ggttttgcat
    13681 tcactcttgg gttaacaact cttgttgact tggtggttat cttcttattc accaagccgc
    13741 ttgtaacgct acttgcaaag aataagtact tccaagctgg aggtaggtac tctggtgtct
    13801 cgccacgcag cattggagtg gtaaccccaa ctctagagag caagggagcg taaaccatga
    13861 aattaactgg tcttggcgga aggctctatc gcggagaaac atcaatgaat gtcatccgtg
    13921 cacgaaagaa atggtatgca gttagcgctt tatttattgc gctctcagtt tttgcactag
    13981 gggctcaagg tttgcgtttt ggaattgaat ttgagggcgg atcagccttt gttgtttcaa
    14041 cctcaactgg aaccgttgag caagcaagag aggcagtatc tgctgctggc tatagcgata
    14101 atgcaattat tcaaactctt ggcgaggaaa aaattcgtat ccaaactgga gtattagatg
    14161 ctgctcagtc aaatgcaatt caagatgctt tagcagctga atttggggta tctgtcgagt
    14221 caatcgatac gcaaaatgtt ggcccttcat ggggtgagga aatttctaaa aaggcgctac
    14281 agggacttat cgcattctta atctgcatca tgatttatct ggctcttacc ttcgagccaa
    14341 agatggctgt ggcagctgtt gtggcgctga ttcacgatgt atttatcact gttggcctct
    14401 atgcatttat tggctttgag gtgacgccgg cgactgtcat cggattctta acaattctgg
    14461 ggtattccct atatgacacc gttgtggtct tcgataaaat tcgagaaaac acaaagggca
    14521 ttactagcac tggaaaatct acctactctg atgcaacaaa tcttgcggtt aaccagacct
    14581 ttgttcgttc tctaaacacc tcacttattg cgctccttcc agttgcagca attctctttg
    14641 tcggagcagg ccttctaggc gctggaactc ttaaggatct agcgctcgct ctctttattg
    14701 gtctaattgc tggaacctat tcatctattt tcattgctcc tccagtgttg gcgtctcttc
    14761 gtgagcgtga gccagcaatg aaggctctaa gtgttcgtgt cgctgcaagg ggcggaagta
    14821 caacaagccc actactttcc aatcgaaaag gaaagaagaa gcgcaacaaa taggggagaa
    14881 gtaacctatc cccatgaacg ccttaattac cccggtggtt aatcgcctta aggagagcca
    14941 agagggtagt gacacttcgg ttgttgaacg cgcctttgaa gttgcagagc gggctcataa
    15001 aggccagtta agaaaaagcg gcgatccata tataactcac ccggttgcag ttgctgagat
    15061 ccttcttgat tttggtcttg atgcaaatag catcgcagca gctctacttc atgacacggt
    15121 ggaagatacg agttattctc aagaatcact tcgcaaagat tttgggcctg aggttgcctc
    15181 acttgttgat ggcgtaacaa agctagatcg actgacctat ggtccaactg cggaggctga
    15241 gacagttcgc aaaatggtcg ttgctatggc aaaagatatt cgagtcttag ttattaaatt
    15301 agctgaccgt cttcataacg ctaggacctg gcaatatgtt gagccagaat cagcagagcg
    15361 aaaggcaagg gagactcttg atatctatgc tccgctagcc catcgcttgg gaatgaacgc
    15421 agttaagtgg gagttagagg atctctcctt taaatatatt gagcctaaga aatttgaaga
    15481 gattgaacgc ttagttcaag agcgaaaccc ctcccgggct aagctaacta atgaggttat
    15541 tgagttagta gagcaagact tagcatcgga gtcaattgct gcaaaggtaa ctgggcgaca
    15601 taaacatctc tatagcgtct atcaaaagat ggttatcaga ggacgagaat ttaatgatat
    15661 ttatgatcta gtcggaataa gagtgttagt tgatgatgtc cgagattgct acgccgttct
    15721 tggagcgatc catgcaagat ggagcccggt tcctggaaga tttaaagatt acattgcaat
    15781 gcctaagttc aatctctatc aatcacttca caccacagta attggtccta atggaaaagc
    15841 cgttgaaatt cagatcagaa ctcacgatat gcactcaagg gctgagtatg gaattgcggc
    15901 ccactggaaa tacaaattat ctggctcctc aagatcatcg gattcgccag acatgctctg
    15961 gttaaagcag cttcatgaat ggcagcagga aacctcggat gtcaccgagt ttctcgatgc
    16021 tctccgattt gatcttagaa ctcctgaaac gtttgtcttt acgccaaaag gaagcgttat
    16081 cgcacttcca ctaggtgcaa cagcggtgga ctttgcctac gcagttcata ccgatgttgg
    16141 aaatcgatgc ataggagcaa aagttaatgg caaattggta tccctcgaga tgccgttagt
    16201 aaatggagat gtcgtagaag tcttaacaaa taaagccgaa actgcatctc ctagtagaga
    16261 ttggcttaat tttgttaagt catctagagc tcgctccaag attaaggctt ggttttctaa
    16321 ggagcgacgt gaagaggcaa tcgatgcagg aagagaatct attgcaaggc agatgcgaaa
    16381 agcagggcta ccgcttcaaa agatccttgg cggacatagt ttgcttcaat tagctcacga
    16441 tcttaattac ccagatatag aggcgctata tgctgctgtt ggcgatggcc atatctcagc
    16501 aaatcatgta attgaaagac tcgaatcatc ccttggttta gctgagacct atgacagccc
    16561 aatagatgaa aacataacta ctgcatcgcc aagtcgcatc aatcgaagaa gtgcctctgg
    16621 cattatcgtt gaaggcgttg atgatgtatt ggtgaaacta gcaagatgct gcaccccagt
    16681 gccaggtgat tccataactg gattcattac tcgcggcaat ggcgtatcgg ttcatagaag
    16741 tgattgcgta aatgtcgcgg atttgaaatt tcatcaaggc gatcgcatca ctgcagttaa
    16801 atgggatgag ggagcaaaat ctgtcttctt agtgaatatt cagattgaag ccctagatcg
    16861 cgcaaggttg ctctctgaca ttacccgcgc tctatctgac caggagatca atatcgttca
    16921 tgcggccgtg aataccacta aagatcaggt agctttttca cgctttacct ttgagatggc
    16981 agatgcctca cacctagagg cggtcttggc ttcagtaaag aaaattgaag gcgtctacga
    17041 tgtttatcga attaccaaca attaagattg aaagtcagcc agaccttttt gggcttcgct
    17101 aagccacata cgtctggcag cggcagcctc aagtaaatca gcagccttct tagaatctcc
    17161 attagcttga gctttagcag ctttggcttc atattcggca acagcatttg ttagttgagt
    17221 aacgacatcg ttagctcttg caattgcagt tggatcgcta cgacgccact gctcttgttc
    17281 agcctcttta atagtcatct ccacgctatc aacgcgttta tccaaaacgg atcgtttctt
    17341 gcggtcgatc atgccaatct tggagagctt ctggcgaaga tcgcggaatt tgcgtctaac
    17401 atcatcaaga tttgtaattg gaagaagtgc ttccatttca gcaacaattg cttcgcgctt
    17461 ttcaaggttt gcagccatcg attcgccgcg cttttccaga tcggcatttt ttgaagtgaa
    17521 gaaagtgtct tgggcagctt taaatctggt ccagagcttg tcatcatcac tcttctttcc
    17581 tcgaccgctt gccttccatt gatccatcag agttttatag cgtcgagcag ttgctaccca
    17641 atcagtagat gttgcaagag attcggcttc agcaacaatc ttttccttag catccttaac
    17701 ttggctctgt tgagcgctaa gacctgcaaa atgaactctg cggcgtttat caaacttatt
    17761 gcgcgcagat gagaagcgct tccataactc tgaatctgtc tttttatcga gtctaggagc
    17821 tttcttccat tcatctagta aaactttaag gcgctcaccg gtaactttcc agctagttga
    17881 atcgattaga ccttcggctt cgacaactag agcttctttt gcggcgatag ttgctgcgcg
    17941 cttttcagct tttgcctctg cctctgctgc ctttttcgcc tcgtaggctg gacggtgttc
    18001 atcaattagc ggtggaatct gctcgagtga acttcttagc gcttcaaggt ttccaacgcc
    18061 atttagatgt tcaatttgat tccttaactt cttaactgcc tcatttgcat cttgaggaac
    18121 catagcgccg ctgataattc ttgcggcaag tagagcaact tcggatgcga caagttcaaa
    18181 tttacggacg aagtaggcaa gtgcctcttc agcagatttt ccaggatatg aaccaactac
    18241 tttctcccca tcgctggttc gaacataaac cattccattt tcatcaacgc gaccaaattt
    18301 tgaaggatca ccgattagcg ctgcagcagc tgagttaggt agaacttgat tatctagagc
    18361 atccattggg tcctactttc tcgaacgttg cgcgcctgga gtttttcact ctggcgttat
    18421 gggttcagcg gttggtaagg gccaaacgct accgccatag ggcgaatctg taaaaaagtc
    18481 gttcacgtgg ggctcttttg agcggaagtg ggcgccgggg ttggaagaat gcccaggtga
    18541 gcctcttagt ggatcgagta gttgcgcctt actacgctac aaattgttgg atcatagctc
    18601 cagcaaaaaa ttcacactgc gtcgttattg atcccggaat tgccattcca aatcttatga
    18661 aagaaataaa agcaaagctt gaggaacata atctagttgt gggagcaata tttattaccc
    18721 atggccatct tgatcacaca ttttctctct actctcttgc taaagatttt attgaaagtg
    18781 atacctatat tcataaagct gatagggacc tactaagtaa tccagaacgc gcaatgggac
    18841 cgcaatccca agcacttttg agcgaacttg gaaagctttc gggggaggga attaattttc
    18901 aagaaccaga gcgaacctgg tcaatagatt ctgatagcga atcaaaactt gggggaatga
    18961 atttctcctt tagacatgcg ccaggacata ccccaggttc tactctggcc atagtcgatg
    19021 atgagctatt aatcagtgga gatgtgctct ttaaaggagg aattggcaga acagatctgc
    19081 cacgtggctc aatgagcgac atggagataa ctcttcgtga gaaaatagcg accttgccac
    19141 ctcatcttcg agttcttacc ggacatggcg atgagagcag gatggaagag gaattgaaga
    19201 gcaatcaata cctaatagcg gcaactcaag ggagattggc atgagtcaaa agtttgcagc
    19261 ccctaagggc gtctctgagt atttgccgcc aaaatcaaat gtttttgatc ggattattca
    19321 aacgctaaaa gtcccagcac ttgctgccgg atatgaatta attgagctgc caatatttga
    19381 agataccgag ttatttactc gaggagttgg cgcctcaaca gatgtggtga caaaagagat
    19441 gtataccttt gaagatcgtg gcggtcgctc actaactctt cgccccgagg gaacagctgg
    19501 agtaataaga gctgtgattg aacatggatt agatcgtggc agcttgccag taaaactctt
    19561 ttatcatggg ccctttttta gagctgagag acctcaagct ggtcgctatc gccagtttta
    19621 tcaatttgga attgaggcca ttggtagcaa tgatccatat cttgatgcag aggtaatctc
    19681 gattgcctat caaggttata gaaaacttgg acttaaagat tttgaactcc agataacctc
    19741 ccttggtgat agtgaaagta gggcgacaca tagaaaagat ttagatgctt ttatcgctgg
    19801 gctaaatctc gatgaagaaa cccgcgcaag agcgcttaag aatccgctgc gcctctttga
    19861 tgataaacgt gaagaaatta gagcatccat gaataaagcg ccactacttc ttgattattt
    19921 gaatagcgaa agtagagaga acttcgaagc ggttaaatct gctcttgatt ctttggaaat
    19981 tagctatgta gtaaatccaa gaatggttag gggccttgat tactacaccg gcacaacctt
    20041 tgagtttgtt cacaatggac taggcgcgca atctggtatt ggcggcggcg ggcgctatga
    20101 cggccttatg gagctgcttg gaggatcaaa gctttcagga attggttttg gattaggaat
    20161 tgatcgaatc ctgctagccc ttgaagcaga ggcaaagagt ggacaattgc tccctggaga
    20221 attgaaatat atcgacctct atgtgattgc tctaggtgat gaggctcgct caaagtgctt
    20281 tactctcgtt gattcactta gaaaagaggg ctttgttgta gagcagaact ttggagatcg
    20341 ctctctaaag ggtgcaatga aagcagctga taaatctgga gcgcgctttg caatagttct
    20401 tggcgatagt gaattgcaat cagggtcagt tgagctaaaa cagatgagca atggagagca
    20461 gacttcggtt aggcttacct ctcttgctaa gacattggcc gagaagtcat ctgggcttta
    20521 acaggaatag agagtaggtt ttttaattgc ttcgctccca tagcgctgga acccttcgat
    20581 caagtgatat tggcagcagg gtaaaacttg ctggctgggt tgcaaggcgt cgcgatcatg
    20641 gcggtgtggc atttattgac ctgcgcgatt actcgggaag tgtgcaagta gttatctcgg
    20701 atgaaaaagt ggctgctgct cttagagctg aatggtgcgt tttaatcgat ggagtcgtta
    20761 ctaaacgtcc aagtggaaac gaaaattcag cactacctac gggcgagatt gaagttaatt
    20821 gcgagagcct tgaggtttta agcgaagcag ctgcgctgcc atttcctgtc gatagcggcg
    20881 aagtagtaaa cgtcagcgaa gaggtaaggc ttaaataccg ctatttagat ttgaggcgcg
    20941 agggaccaag tagcgcgctg cgccttcgtt caaaagtcac ctcaatcatt agatctgtaa
    21001 tggatagcga aggcttttta gatattgaaa ctccaaatct aactcgctca acaccagagg
    21061 gtgcaagaga tttcttggtt ccagttcgtt tgcagcctgg gtcttggtat gcccttccac
    21121 aatcacctca gctctttaag caacttttaa tggttgctgg aatggagcgg tattaccaga
    21181 ttgcacgctg cttcagagat gaggacttta gggcggatag acaaccagag tttactcaat
    21241 tagatattga gatgtcattt gttgatcaaa gtgatgtcat tgctgttgct gaaaagatat
    21301 tgaaggaagt atttaagaga actttagata tcgatatttc tctaccaatt gctcacatga
    21361 cttatcacga agcgcttaga aggtttggct cagataaacc tgatttacgt tttgaaaatg
    21421 agttagttga tgtaactgat ttctttaaag agactagctt tagagtattc caggcagagt
    21481 tcgttggcgc tgttgtcatg cccaaaggag ctgcatcacc aaggcgtgag ttagatgcgt
    21541 ggcaagattg ggccaaagct agaggcgcta aaggtttggc atatattctc gttggcgaag
    21601 atggcagcct gggtggcccg gttgctaaga atctttcgga gaaagagagc aaggagattg
    21661 cctcctttgt cggtgcgaaa aatggcgatg ctattttctt tgctgcaggt gatcgctctt
    21721 cttcgcttca acttcttgga gcagttcgtt tagaaatagg tcagagatgt aatctgattg
    21781 cagaagatca atggaagttt ctctgggttg ttgatgcacc gatgtttgaa gcaaccgatt
    21841 ctgggggatg gaccgcggtt catcacccgt ttactgggcc aaagccagaa tatgagaaga
    21901 gctttaaaga tgatcccaaa tcagcccttg cttatgccta tgacatcgtt ttgaatggaa
    21961 atgaaattgg tggcggttcc attcgtatcc ataagcgcga aattcaacaa gctgtatttg
    22021 atgttattgg attaactaaa caagaagctg aatctaaatt tggcttccta cttgaagcct
    22081 tcaactacgg tcctccgccg cacggcggaa ttgctcttgg cctagatcga ctatgtgctc
    22141 tacttgccgg cgctgagtcg attcgcgaag ttatcgcctt tccaaagacg gcaagcgggg
    22201 cagatccact aactggcgct ccaactccga ttactaccgc acaaagacga gagagcggag
    22261 tggatttcaa gccagaataa ggcgagaaat ctttagtagg ctaacgctat gtccgcaggt
    22321 ggaattgcag caataatcgc agctagcgct ctattaatta ttggtatcgc tatcgcctac
    22381 gccgtcattc gactcggaag agctatcgac gaggttggtg gagcagttcg agatattgct
    22441 aaggagacag cgcccctgct caatgaagtc acaactactg tagagcttgt taatggaccg
    22501 ctgcagagca tcaataaagt aactaagacg atggaggagt tcagctctaa agtctcagag
    22561 agcgcttcag gctttcttga taagaatcaa atggcgatga aggcagcagg tgctgtgtta
    22621 acggctgctc aacttcgtaa aaagaaaaaa gggaagaaga gtaagaaggc taaaaacagc
    22681 aggtatgttg acgacgagga gttctaactc ctcgtgaatt cagcagaaat tcgttctcgc
    22741 tggctaaaat tctttgagtc agcaaatagc caaggcatta aacatactgt tgttccttca
    22801 gcttctctga ttgcagatga tccaaatctt cttctagtta atgctggaat ggttcctttt
    22861 aagccgttct tcctagggga gataactccg ccatataagc gcgctacctc agttcaaaag
    22921 tgtgtcagaa ctttggatat tgaagaggtt ggaaaaacca cacgtcatgc atcttttttc
    22981 cagatgtgtg gcaatttttc ttttggtgat tacttcaaag agggagctat cactcttgct
    23041 tgggagctat taactaaacc tataagtgat ggtggatatg gatttgccga agataaattg
    23101 tgggtcacgg tttatctaga tgatgatgaa gcagctgaca tttggcataa gaggatcgga
    23161 atccctaaag agcggatcca aagacgtggg atggcagata atttctggtc aatgggcgtg
    23221 cctggtcctt gcgggccttg ctctgaaatt tatttcgatc gtggagctgc ctacggcaaa
    23281 gagggaggtc caatcgctga tgaggatcgc tatctagaga tctggaatct ggtttttatg
    23341 caaaacataa gaggagaagg cggaggaaaa gagagtttcc caattcttgg cgaacttcct
    23401 gccaaaaata tcgatactgg cttaggtctt gaacgcactg ccgctttatt gcagggagtt
    23461 gaaaatatct acgagataga tacaactcaa cacattctta atcgcgccag tgatctaagc
    23521 ggagttacat atggcaaagc cacatcaagt gatgtatcac ttcgtgtaat tgctgatcat
    23581 gcaagaacgg cgacaatgct cattggcgat ggagttactc caggcaacga aggtcgcgga
    23641 tatgtcctga gaagaatgat gcgtcgaacc actaggaata tgaagttgct aggcgccgat
    23701 gatcgcaata tgagcgaact tgttaaagcc tctattgaag cgatgggtcc gcaataccca
    23761 gaattgcaga gcgatagtgg tcgaattttg aaggtggccg aagctgaaga agagagtttc
    23821 ctgcagacct taaagagcgg aactttaatt tttgaccaga gaagtgcttc cttgaagaaa
    23881 agtggcgcaa aagttctctc tggcgaggat gtatttcaac ttcatgatac ctatggattt
    23941 ccttttgatt taactcttga aatggcaaat gaagaaggtt tgagtatcga tgccgaaggc
    24001 ttcacgagat tgatgaaaca acaacgcgag agagctaaag cagatgctaa atcaaaaaag
    24061 agtgggcata ccgatgtcac tgagtatcgc aagatagctg agagcgctgg aaagttagaa
    24121 tttgtcggct atgagagcca agaaagcgaa gcaaaaattg ttggcttcct ccttgatggc
    24181 gcaggagtta agagtgcatc agctggtgat gatgtcgaaa tcgtgcttga tagaacaccg
    24241 ttttatgcag aaggtggcgg acagcttgcc gattctggag tgatcacaac aagtgatggt
    24301 tctgtcattg aagttgaaga tgttcaggcg ccaattccag gtgtatttat ccatcgcggt
    24361 cgagtacttt ctggccaatt ggaaattggt gaatctgttc tagcaaaaat tgatagcgat
    24421 cgtcgcaaag caatttcaag agctcacacc gcaactcata tggttcataa ggcatttcga
    24481 gaaattttgg gggagactgc tactcaagct ggatcggaga atgcacctgg tcgttttcgt
    24541 tttgatttcc catctagcgg cgcagtttca gaaggagtcc ttaacgatgt tgaggctcga
    24601 gtaaattctc ttcttctaga gaatttgctg gtcagcgctg aagagatgtc gcaagcagag
    24661 gcaaagaaaa taggcgcaat ggcgctcttt ggggaaaagt atggtgagcg agttcgagta
    24721 attagcgttg gcgattgggc taaagagcta tgcggcggaa cccatgtttc aagaagtgga
    24781 gagcttggaa tagtaaaact tcttggagag tcatccattg gagcaggggt aagacgcgtc
    24841 gaggctttag ttggagccga tgcctactcc ttcttgagcc gtgaacacat tttgatcaac
    24901 tctcttcttg aaatcattaa ggcgacacgt ccggaagagc ttccagagag aatctcctct
    24961 cttcttgcac gattaaaaga ggccgaaaaa gagattggct ctatcagaac agccaaggcc
    25021 ctaagtgatt cagcggcgct tgtaaaaaac gctgaagata tcggtcaatt taaacttatc
    25081 tctgctgaca tgggtaaagg cctgtcaggg gatgatttga gaacagttgc gctagatcta
    25141 agaaatcgcc tgaagggcgt agttgttgca ctgcttagta gcggtgatga aaaagtaacg
    25201 ctagtagttg caactgatga aactgctcgc agcgcaggcg caaaagctgg agccttggta
    25261 aaaatagctt ctggaattct tggtggtggt ggcggcggaa aagatgattt tgctcaaggt
    25321 ggcggaagtg attttggcaa gatagatgaa gctttaaagg ccatcaaggc cgcgctctcg
    25381 agtatctagc ttttgtgatg atccaaggcg cgaggcttgc tattgactac ggtgagaggc
    25441 gaattgggat tgcaaaatct gacaactctc gattgatcgc atccccagtg gttactctcg
    25501 acaatagcga atcaacaggt aatgtctatt ctgcaatcaa ggatattgtt tctgagagca
    25561 atgcgatgtt ggtctacatc ggtcttccac ttcatctctc cggaaaagag agcgcatcaa
    25621 gtgagaaggc gagggtattt gcaggcctac tcaaacagca tctaccgcaa gaggttcaaa
    25681 ttcgacttct agatgaacga ctaacgacaa gctctgctgt tggcgagtta aagaatgctg
    25741 gactcaaaag cagtagggag agcattgatc aattggccgc tgtgaaaatt ctagaatttg
    25801 ctctggaagt tgaacgctta agtgggaaag ctgctggcca tgaagtttag ggcagcttct
    25861 ataaagcaat cacttgagag tagtccacta ctacggttag cgactgcggg gattttccta
    25921 ctgcttttaa ctctctcact ccgtgaactt aattcgtctc ctcagagcgc tcctgatttt
    25981 gaggcaggat ctgctggcga agaagtcatt gttgagattt tatctggtga aagcggatcc
    26041 gaaattggta aaaaacttga attgctatct gttgttaagt cttcaaccgc attttttagg
    26101 gtagcggtcg ctgatcctag atcacagcgc atagcccctg gtgaacatcg aattgaaact
    26161 agaattcctg caagaactgc tttggagcag ctcttagatc catctcgtat tccaaatcta
    26221 atagttgtca gagatgggca gcgtttatct gagatatcag agagcattat caatttcgga
    26281 atttcaaaag cagaacttga aaagagcata aaggctttct ctccgcccaa gatctttaag
    26341 tctaaaaata tcgagggctt tctctatcct gctcagtatt ctttcaacaa gagtgtagat
    26401 gcagatgaaa tagtttcggc tatgcttaaa aggtttagct ctgccaccaa ggatctcaac
    26461 tggaaaaacg aaagagagct cacacctttt gaagtgatgg tgatcgcatc tcttgtgcaa
    26521 acagagggca cgccagatgt ttttggtaaa gtttctcggg ttatttataa tcgattagaa
    26581 aagcgcatgc cattgcagtt tgactcaaca gtgcactatg ccttgggtcg tcgtggagaa
    26641 atacgggttt ccctgaacga cataaaggtt aagaatcgct acaacacatt tatctatcca
    26701 gggttgccac ctgggccgat aggaagtcca actaaagcag caattgacgc agctttaaac
    26761 cccgaagcag gaaactggct ttactttgta acagtaaaac ctttcgatac tcgatttact
    26821 aattcttatg accagttcct tggttggaag agtgaatata agagaaactt caaagccggg
    26881 ttattcgaat gatgaaacgc gcagcagttc ttggatctcc catttcacac tccttatctc
    26941 ctttgattca taatcatgcc tattcgcttc ttgaataccc tgcaatttat gaagcgatag
    27001 aggttaaaag tggggaactt agcgaatttt taacaaggga actgaaaagc agtgatgtgt
    27061 tgggcttttc tctaactatg cctcttaaag aagaactggc tcaattatca ataccagccc
    27121 ttcaagtcga ggctcgagca aaggcgatca attcagcaaa taccatctac aaggatgggc
    27181 aaagctggaa agtatcctca actgatgttg atggatttga attccttctc aaagagctct
    27241 cattctcaac ggtatcgata cttggagcag gagggacggc aagagcggcc atcgcatcac
    27301 ttagtgatag agatgtgaag atagagatct atagaagaag tacaagtaga gatgcctcgc
    27361 ttagtgaagc cgctatgagt tctaaaattg aaatctacga ttgggataaa tgtgctcgag
    27421 cttggaagag tgatttagtt atcaacacca ttccatcaga ccaagttcta tcgatcatta
    27481 agggatttag ctctccaaaa atacttctcg atgcaactta ttcgccatgg cctccagggc
    27541 taactaaaca acaattggac gctaagggct tattggtttc aggtctgcaa ttacttgcgg
    27601 ctcaggctgt ctatcagata tcgcttatga ctcaaatcga ctttgataaa acctggctat
    27661 ataacgaact gattctcaaa ttagttagtg cacaatagcc ctgattgtgg ataaggctgt
    27721 ttagcttttt tctttcgaat taaagttttc gtgtgctctt catacacctg accttcttcg
    27781 gacttgcaat ttcctggaga gattatcaaa ctcatctgat atcaaaaagg actacgcatt
    27841 tagctttact tactcttatt ccattcattg ataactcaat atttagggca ctcttactct
    27901 catcaataaa tttatgcctg tatctgttct taaaactgat atcaccacag gctatcggtc
    27961 gtggtgatat tcgactttcg cttctaatcg gttcttacgt ttcggccttt gatggaggcg
    28021 tatatgaatt gatctggtgt aacaccatca gttgggtctt ctgctcgatt tctctaattc
    28081 ctcattttct aaaacaggag cggcttaagg aaatgcgtgt tgcttttgca ccttatctct
    28141 tcttcgggct cctttgctat gtaatcacta gttgagcacg catgaaaaat aggtcacgaa
    28201 tttcaggatt aatctgcgat aattgagcaa tgagatggtt aacagctgga gaatcccatg
    28261 gaccagcttt ggttggaatt ttagagggaa tgcccgcatc tgtttcagtc acaacagcag
    28321 atattgatga ggatcttcgt agacgcagac ttggttttgg caggggagca agacagaact
    28381 tcgaggcgga taaagtgaca atacttgggg gagtccgtca tggcttaacc caaggcggcc
    28441 cgattgcgat tcaaatagca aactcagagt ggcccaagtg ggaaaaagtt atgagtgctg
    28501 atcctgtaga tgaagcagag attgaatcat tgggaagaaa tgctccactg acaagaccaa
    28561 ggcccggaca tgctgattta gttggcattc aaaaatatga ttttgatgat gcaagaccag
    28621 ttctggagcg agcgtcagca agagagacag cggctcgagt tgcactcggc gctgttgcac
    28681 ggagatttct taagcaggta ggcggcatag aggtatttag tcatgtggta tcaattggag
    28741 cagttagtgt tgccgatgat tacttgcttc ctgagccaaa ggaccgcgag agaatagata
    28801 gcgatgaagt ccgctcggca tttaaagatg ttagcgccaa gatggttgaa gaaatcaatg
    28861 atgcgcataa aaatggcgac actttaggtg gggtagtaga agttttggtt tacggcctac
    28921 cgccaggact tggttctcat gttcactggg atagaagact tgactctcgt cttgcgggag
    28981 ctctgatggg tattcaagca attaagggcg tagaacttgg tgatggattt actacagcaa
    29041 gaaaacgcgg cagtagcgcc catgatgaaa tagagagaga taacttagga aatattcgtc
    29101 gaagaacttc tagatctggc ggcactgagg gcggaatgag tactggagaa gttttaaggg
    29161 ttcgcgcagc aatgaaacct atttcaacag ttcctaaggc tttagatacg atcgatatca
    29221 caaacggcca ggcagctaaa gcaataaatc aaagatcaga tgtatgcgct gttccagccg
    29281 caggcattgt tgctgaggcg atggtggctc tggttttagc tgaagcgatg cttgaaaaat
    29341 tcggtggaga ttctcttggt gagagcaaga gaaacctaga aaacttcctt tcttctatga
    29401 aggtgaaata aggcgtgcca ccgattattc tcatcggacc accaggagct ggaaagacct
    29461 cagtaggaaa agctcttgct aagaaacttt cattgaactt tcttgatagc gacaaagtag
    29521 tggaagagaa aagtggaaag agtatctctg agatcttcat aactgatggg gaaccagcat
    29581 tcagggagat ggagagagct gcagtaatag atttgattga gaaccaagat ggcgtgatcg
    29641 cactcggcgg aggttcggtt atggatcttg aagtgtccaa aaggttattg ccaatggcaa
    29701 atgttgtttt tcttgatgtt tcaatctcga acgcagcacc aagagt
//
LOCUS       DAOROD010000013        28996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_49_length_28996_cov_26.174942, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000013 DAOROD010000000
VERSION     DAOROD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 28996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28996
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_28996_cov_26.174942"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLW53_03860"
     CDS             <1..396
                     /locus_tag="PLW53_03860"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRD36590.1"
                     /translation="SLNKKVDAIVGIESRGFILGGAIAKALEIGFVTVRKPGKLPRDV
                     YRKDYALEYGFDALELHKDLLDPSDNVIIVDDVLATGGTALACHDLVEITGAKVVAHL
                     FMLEIEFLQGRKMISDTHSEVGIISLLKA"
     gene            414..1055
                     /locus_tag="PLW53_03865"
     CDS             414..1055
                     /locus_tag="PLW53_03865"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRD36591.1"
                     /translation="MAELIYYCGTMDSGKSTLALQTAHNHQSRGRAGLLFTNRDRAGS
                     GKISSRLGLTSEALEVSLEMDLHKFVVDQLSTGRRIDYVICDEAQFYAPEQVEQLARI
                     ADGLGIEVYAFGILTDFRTKLFPGSARLIELADRVQTLQVEALCWCGRRATHNARTIG
                     GVMVTEGEQVVVGDTNAANEVAYEVLCRRHHMRKVTARNSGAAHQSSDPLPFN"
     gene            1070..2113
                     /locus_tag="PLW53_03870"
     CDS             1070..2113
                     /locus_tag="PLW53_03870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRD36592.1"
                     /translation="MTSTRGYAAMSAKADLTPYAFERRVLRSDDVGFRIDFAGICHSD
                     IHQVREEWGKAIFPMVPGHEIVGTVTSLGSSASRFKIGDRIGVGVFIDSCRECKNCLN
                     GLQQYCLEGMTGTYNGYERNSKEIALGGYSNYFVIPESYAYRLPDNLDMAAMAPLLCA
                     GITLYSPIKKWKVGPGSSVAVAGLGGLGHMGVKFAAALGAKVTVLSHSDSKESDAKAF
                     GATDFVNTSKADWHLGYRKSFDLILNTVSAQIDLEPYLSTLATDGSLVVIGLPGAPYS
                     VSAGSLLDGRRSVAGSMIGGIPETQEMLDFCGAKNIVSEVEVISADYINKAYERTIAS
                     DVKYRFVIDCSTF"
     gene            complement(2110..2841)
                     /locus_tag="PLW53_03875"
     CDS             complement(2110..2841)
                     /locus_tag="PLW53_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HRD36593.1"
                     /translation="MFVGVGTLINILAIIAGAALGVLLGNKMSEKTRNLVMDVLGAIT
                     LIAAAGAIKALWDEDFNRYLPDGSPLLVVLASLLVGGLLGSLLKIEDHLEVIGIKLKA
                     RFDKDGKSPFVEGFVSASLLFVIGPLAILGSISDGMSTGIEQLTLKSILDFFTSIAFA
                     AALGWGVALSALPVGIYQFAWTGVGFALGAILEPYQVSAMTATGGVLLLGIALKLLNI
                     KQIAIGNLLPALALAPLFALIASNI"
     gene            2881..3543
                     /locus_tag="PLW53_03880"
     CDS             2881..3543
                     /locus_tag="PLW53_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36594.1"
                     /translation="MKRFIDALRPHKTIPITPWSASHRWDLSISRVLILTFGLFIFGI
                     GDSLIIISELGNAPWSVLAQGVATRSDITIGSATFLISCLVLLLWIPLKEKPGFGTLA
                     NIVVIAIGIDVGIAFIGTPDLFIIQLLYVFIGIGLIGIGSALYITCALGPGPRDGWMT
                     SLHRRTGIPVGRVRTAIEVSVLLAGWALGGRIGLGTALFALLIGYAVAAGFGVVSRLT
                     KQ"
     gene            3655..5091
                     /locus_tag="PLW53_03885"
     CDS             3655..5091
                     /locus_tag="PLW53_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRD36595.1"
                     /translation="MLDSYFKISQRGSSVAQEVRGGVVTFFTMAYIVALNPLIIGLAK
                     DGDGKFLGGGDVPNLALVAAATALIAGVMSILMGVVANFPLAIATGLGLNTFVAVGIA
                     SKMTWADAMGLVVLEGIIITVLVLTGFRTAVFRAVPTQLKIAISVGIGLFIALIGLVD
                     AGFVRRTGSGPVPVTLGNNGELVGWPVIVFAFGLFLTIGLMVRKVKAALLLGIIISTG
                     LAIALESAFKIGPLFDGATGNVNPKGWNLNVPALPEAVVATPDFGLLGSFNLFGSFDR
                     VPLITALLLVFTLLLADFFDTMGTMTAIGQEAGLNDKDGTPPNADRILLVDSLAAVAG
                     GAGGVSSNTSYIESASGVGEGARTGLASVVTGVLFLLTTFLAPLVAVIPYEAATPALI
                     VVGFLMMTQIKSIDWEDYGIAIPAFLTIILMPFTYNISVGIGAGFVSYVVIRYLQGRR
                     KDVHPLLLLTAGLFMIYFLNNPINVWVS"
     gene            complement(5145..5795)
                     /locus_tag="PLW53_03890"
     CDS             complement(5145..5795)
                     /locus_tag="PLW53_03890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRD36596.1"
                     /translation="MLSSLVPDHWKEVLQDYNSQIDELGLKLQKRADQGERILPDKKH
                     LFRALELKPEQVKVIIVGQDPYPNISDAIGLSFSVPPRKTGLPGSLLNIQKEIMTDIG
                     STTTADGDLTKWASQGVMLLNRVLTVTAGESASHSKLGWQEITEKIVSHCANLGAVGL
                     LWGSSARELAHLFKPDCLVEGVHPSPLSAHRGFLGSKPFSRVNQLLENKGKTAIIW"
     gene            complement(5795..6580)
                     /locus_tag="PLW53_03895"
     CDS             complement(5795..6580)
                     /locus_tag="PLW53_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36597.1"
                     /translation="MEPLLRCVEVEVLRSSKRILGPISWQINAQERWVILGPNGAGKT
                     TLLQLLAALIHPSKGSVEILGEKMGSTDVFELRPRIGFTSSAMMEFLPGDEKVIDVVL
                     TAAYGIAGRWQEDYDLWDESRAKALLNIFGVRELGERTLSTLSEGEKKRVQISRALMA
                     DPELLLLDEPAAGLDLGGREDILSRITSYTSEERAPATVIVTHHIEEIPAGTTHALLL
                     KEGQALYAGAISETLTEANIEEVFGVRVSLSFNGLRYFAVAKD"
     gene            6630..6866
                     /locus_tag="PLW53_03900"
     CDS             6630..6866
                     /locus_tag="PLW53_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36598.1"
                     /translation="MKARAIILISGAASQAPNQLVDETLKPFTLSILDRQEILMAGRM
                     ILALEIELDRAHLDAVESDLIKALDQFDVACELL"
     assembly_gap    6986..7085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7199..7858)
                     /gene="rpe"
                     /locus_tag="PLW53_03905"
     CDS             complement(7199..7858)
                     /gene="rpe"
                     /locus_tag="PLW53_03905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRD36599.1"
                     /translation="MIRICPSILNANFDDLPGEIEKVASTADLIHLDVMDNVFVPNFT
                     FSPERTREIIDFSMLPIDVHLMVAEVDEKVSQYSSKNAVSMTVHFEACQDPLQTLKNI
                     RSLGKRAGLAIKPDTPIEVVEELLPAIDMIVVMTVEPGFGGQSFMASLMPKVEKARRY
                     LEQRKLNDVWLQIDGGISLSTIETARRAGADTFVAGSAVYKSENPGSMVEQLRTLANN
                     C"
     gene            complement(7855..9171)
                     /locus_tag="PLW53_03910"
     CDS             complement(7855..9171)
                     /locus_tag="PLW53_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD36600.1"
                     /translation="MSSKPRRIDDPRLLAFEVLAEVGLQGAYSNLILPKALSESILEA
                     NDRAFATELVYGTLRMQGRHDHFISAASDRSLEQIDPKALIVLRLGTHQLKQMRVPSH
                     AAIYESVELAKKVVGKSTASFVNAILRKIDSLDFEQLVKPTQEYARFALEYSHPEWIV
                     SSYFDSLKNSSDVMSLLQANNNPAKPTLIAWPGLATQGELLEAGALAIASSSVAATFD
                     GNPGDIAAIRERRAGVQDLGSQLVVEKFYDEFKPNLRWLDLCAGPGGKAAYLSALLKR
                     DGGSLLANEISNERAKLVSQVMHHGEVNVSDGRSMPDELGKFDRILLDAPCTGIGALR
                     RRPEVRWRRSLQDLKNLTQLQSELLESATRLLSPGGIIAYVTCSSHQAETKFQIRSFL
                     KQHNDFTRIKVQDERADIDGDLQLWPHRDGTDAMFLSLLRSKESVG"
     gene            complement(9168..10076)
                     /gene="fmt"
                     /locus_tag="PLW53_03915"
     CDS             complement(9168..10076)
                     /gene="fmt"
                     /locus_tag="PLW53_03915"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRD36601.1"
                     /translation="MKILVASSSPLAKDLLNHIKNSDHQLLGGISSPDRASGRGQSVS
                     SNDFASYCQEIGVTCYKPSTNKELADILQKTQVELVITLAYGRLIKTSELQIPKHGWL
                     NVHFSLLPRWRGAAPVQRAIAAGDEQSGVTVFKLEQGLDTGPIYSTLSYPLNDRSRSD
                     EVLKDLSSLCIQPVNQALIMIAAGEPATTQESEGVMLAPKILKSEGRIDWNRDSKMLD
                     RQIRAFQPWPGAYTSLNGTRIEIIEARVSETAGAAGEVISVNPLLVGTGSGSLEIIRV
                     KPENKREMSSSDWLRGARITLPCAFE"
     gene            complement(10091..10651)
                     /gene="def"
                     /locus_tag="PLW53_03920"
     CDS             complement(10091..10651)
                     /gene="def"
                     /locus_tag="PLW53_03920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRD36602.1"
                     /translation="MAIQPIRFFGDPVLTTPATEVIDFDKELQRLVKDLTETMIDAPG
                     AGLAAPQIGVPLRVFVWHVDDVFGHLVNPTLDLGDEIQEGDEGCLSFPELSYQTPRAM
                     RAVAKGFDMHGEPLIVEGTELLARALQHETDHLNGVLFIDRLPPELRKEAMKEIRQAE
                     WFLAASEAGSAPEIRLSPHSTFGKSL"
     gene            complement(10681..12510)
                     /locus_tag="PLW53_03925"
     CDS             complement(10681..12510)
                     /locus_tag="PLW53_03925"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36603.1"
                     /translation="MATDKPLTLRRQKGKSVIRTRQSLDSLQVEVLVDTGVFHLEQPF
                     SYFLPEELKELVDVGSLVRVPFKNDLKNGVVLSVKNKSQAALKPVDSLLTSVRLTPGF
                     LEFTKKVSERYIANQASIFAAVLPTFTKAISPSLGSPLIKQTSGSKPTRRLFLLNRSE
                     SIESRVVRIVKERKGLNLLLILPTQKEIEKVAKALGEMKQEVIEIGSHLTSSQRRKNF
                     ELAASAKPAIVIGTRSAILAPMFHLDEIVIADESVEHYQEQKAPYWNVRDMALLRSEH
                     EHASISFVSHSISLELARLVEIGWISSISERKFLSGHRANIATEPNSYHETIRKALKI
                     GPVLVSVTDKGYANTFSCQRCRNLALCKCGGRLIIGKKNNYSCSICDFQSDQWRCQIC
                     QADQVRIVKGGAEKIVEELGKAFPRVAIFTSTGDKAIAALDGSPAIVVSTFGVEPEVS
                     AGYAGAVLLNGEALLNRAFIRAEEELRFRWFKLAAKVRSSGFIYLSLPSSHPLSQALI
                     SDNVMRAIRAESSEREAVSLPPSVRIIQVTGEERAISGLRRKLLEQFDSLEILTSADN
                     RTIHLKISHQGANALLVSLKALQKYRSASGQDLFSIRVDPYKI"
     gene            complement(12519..13712)
                     /gene="metK"
                     /locus_tag="PLW53_03930"
     CDS             complement(12519..13712)
                     /gene="metK"
                     /locus_tag="PLW53_03930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRD36604.1"
                     /translation="MAKRLFTSESVTEGHPDKIADAISDAVLDSLLSQDPKSRVACET
                     LITTGQVHVAGEVTTEGYADVMGLVRETVLNIGYDSSEKGFDGNSCGVSISIGQQSPD
                     IAVGVDHAFEERVAASKDPLDLQGAGDQGLMFGYATRDTKELMPLPIAMAHRLAERLS
                     SERKSGQLGYLRPDGKTQVTIEYDGDKPVALNTVVISSQHAPEIDIEKKMAPEIKKFV
                     IDPVLASFDLPANDVRILINPTGRFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFS
                     GKDPSKVDRSAAYAMRWVAKNVVAAGLADRCEVQVAYAIGKAQPVGLFVETFGTEKIP
                     VQQISDALLKVFDLRPAAIIRDLNLLRPIYSKTSAYGHFGRELADFTWEKTDKASALA
                     AAVKG"
     gene            complement(13705..14925)
                     /gene="coaBC"
                     /locus_tag="PLW53_03935"
     CDS             complement(13705..14925)
                     /gene="coaBC"
                     /locus_tag="PLW53_03935"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRD36605.1"
                     /translation="MSGEKIIFPKGRQIVLGVSGGIAAYKACDLLRRLQDAGFLVRVI
                     PTQSSLNFVGRATWEALSGQPALSTVWENPAEVSHLAAADSADLIVIAPATADTIAKL
                     AQGRADDLLTTTVLASDAPILIVPAMHPNMWLNRATVQNVETLRSRGFYVMDPEVGRL
                     SGKDFGVGRFPESSKIVTQVLSLTKTELLLEGKRVLVTAGGTREEIDPVRFIGNKSSG
                     RQGLAIAYEALRSGAEVTVIAGHTDPFELDGVRVIKVNTALEMMSAVESEFSSADALV
                     MAAAVADARPVSSSSTKIKKADFDSINLTTNPDILSKVAQSKRSNQVVVGFAAETAKD
                     LIRAGGEKLISKGADLLYVNDVSGGAIFGQDQTSGTLIGLGVDSIDFNGVSKYEVAKS
                     IVIQMAKKLEKING"
     gene            complement(14934..15191)
                     /gene="rpoZ"
                     /locus_tag="PLW53_03940"
     CDS             complement(14934..15191)
                     /gene="rpoZ"
                     /locus_tag="PLW53_03940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRD36606.1"
                     /translation="MATLDGITNPPIDELLDKSGTKYSLVLYAAKRARQINAYYSQLG
                     EGLLEYVGPLVETHVHEKPLSIAMREINEGLLTIENLEPQS"
     gene            complement(15241..15792)
                     /gene="gmk"
                     /locus_tag="PLW53_03945"
     CDS             complement(15241..15792)
                     /gene="gmk"
                     /locus_tag="PLW53_03945"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRD36607.1"
                     /translation="MSKRGKLIVLSGPGGVGKSTIVSKLIDNPNFFFSVSATTRKPRA
                     GEVDGRSYHFIDDKSFDELISKNLLLEWAEFAGARYGSIKAPIDQALSEGKNVLLEIE
                     IQGARQVKAAMPEATLVFLQPPSFQELEARIVNRGTDSPERIAARLDLARSEMAAAKE
                     FEHILVNHRVEDVLEALLSLATS"
     gene            complement(15792..16106)
                     /gene="mihF"
                     /locus_tag="PLW53_03950"
     CDS             complement(15792..16106)
                     /gene="mihF"
                     /locus_tag="PLW53_03950"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HRD36608.1"
                     /translation="MGAPPNLTTQERRDALKAAVAARRTRAAFKEEIKVGKKSWKDAF
                     SAQDESIKKMRVRELIESLPGVGEVRAKNILEKAGISPTRRVGGVGRSQYEKLLELMK
                     AN"
     gene            complement(16124..16813)
                     /gene="pyrF"
                     /locus_tag="PLW53_03955"
     CDS             complement(16124..16813)
                     /gene="pyrF"
                     /locus_tag="PLW53_03955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRD36609.1"
                     /translation="MRNSSPVILALDFNDMASTVEMINLTKEHIGIYKLGLEFYLSHG
                     KSGVNEIQDKFTDIEIFLDLKLHDIPNTVAGACRSVADLNPKFLTVHASGGSKMISAA
                     SSTLPKVEITAVTILTSLDKEQMVAMGLNENIENLTLSLAKNAVNSGARAIVSSPQEV
                     SLLRKHLGEKVTLITPGVRPSGAERDDQERIMTPREAIEAGADFVVIGRPITKASDPK
                     QAAEAITASLR"
     gene            complement(16803..17744)
                     /locus_tag="PLW53_03960"
     CDS             complement(16803..17744)
                     /locus_tag="PLW53_03960"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRD36610.1"
                     /translation="MDSQFQAFDPRCKFGGKFFPSPIFTASGCAGSGKELSQFFDLDE
                     LGAVVTKSISVKPRSGRATPRMAETPSGMLNSIGLQGPGIDQFLAEDIPWLISKSARV
                     IVSISGETIEEYASLARKLRGVAGISGIEVNISCPNVENRGMVFACDPVASKSVVESV
                     RRNVGGELPIIVKLSPDVTDIVSIAGEVISAGADGLALINTLLGMAIDIDAMRPKLAG
                     KTGGLSGPAIRPVAVRAIYQVHAAFKNTPILGMGGVASGRDALELIMAGASAVSVGTA
                     NFGDPTAVMKIKNELIDLLKERKFNSVAAAIGVAHEK"
     gene            complement(17737..18597)
                     /locus_tag="PLW53_03965"
     CDS             complement(17737..18597)
                     /locus_tag="PLW53_03965"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRD36611.1"
                     /translation="MSSINKRAEQRSAEIISNKKVGAYHHMVFAAGDMAAHCRAGNFV
                     AISVGGTGSSMILRRAFAIYRASDRGQYGGTIELVVAPHGQGSRWLTSLEIHDVVNIV
                     GPLGTSFGIPTEPASTLLVGGGYGSAPLFGLAEVLKERGCRVDMVLGASTAGKIYAPL
                     EGKRSVNSLTITTEDGGTGIKGRVTQVLPGLIEKHGSEIIYSCGPMGMLAAIDEIATT
                     AGLLHQCAVEEAMACGIGICMTCVLPVKDSTGVIKMTRSCIDGPVMDGSKVIWDSSRK
                     VPQGTWGFNG"
     gene            complement(18594..21851)
                     /gene="carB"
                     /locus_tag="PLW53_03970"
     CDS             complement(18594..21851)
                     /gene="carB"
                     /locus_tag="PLW53_03970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRD36612.1"
                     /translation="MPLDKSIKSVLVIGSGPIVIGQACEFDYSGTQACRVLKAEGIRV
                     ILVNSNPATIMTDPEFADATYIEPITLDFLEQVIAKERPDAILATLGGQTALNAAITL
                     HENGILEKYQTKLIGADVAAIRRGENREEFRAIVAKIGGESAKSIICHSMDECFKAAS
                     ELSFPVVVRPSFTMGGLGSGIAFSRDELELIAGAGLRFSPVNEVLLEESIIGWKEYEL
                     EVMRDNHDNVVIVCSIENIDPMGVHTGDSITVAPALTLTDVEFQNLRDLSIAIIREVG
                     VATGGCNIQFAINPANGRVVVIEMNPRVSRSSALASKATGFPIAKIATKLAIGYSLNE
                     IPNDITKKTPASFEPTLDYIVVKIPRFAFEKFPEADTRLTTTMKSVGEAMAFGRSFPE
                     ALQKAMRSLEKKGSSFSWDISESLEDLMAKIATPTQDRLLQVQRALALGASIEEVFAR
                     CAIDPWFLSQIAEINSKASEIKAAGDLSLELLRSAKEMGFSDQQIAQLRGTNASEILK
                     VRERLGLFPVYKTVDTCAGEFEAFTPYHYSSYDLESEVKPRSKPAILILGSGPNRIGQ
                     GIEFDYSCVHACFALSEAGYETIMINCNPETVSTDYDTADRLYFEPLTVEDVLEVYRA
                     ELAAGPIAGVIVQLGGQTPLGIAAELKSAGVPILGTTPEAIHLAEDRGAFGKVLEENS
                     LRAPKFGTGFSYSDALKVASEVGYPVLVRPSFVLGGRGMQIVYDDASLEEFITIAAQI
                     NPEHPVLIDKFLDDAIEIDVDALYDGNELYLAGIMEHVEEAGVHSGDSACVIPAQSIT
                     KELIAEIREATKKLASGIGVLGLINIQYAISESKLYVLEANPRASRTVPFVSKATGRQ
                     VAKAAALIATGVKISELRESGMLPEIGDGVASGISVKEAVLPFNRFRRVDGEGVDTVL
                     GPEMKSTGEVMGIASTFGAAFAKSQIAAFGALPQSGAIFISLSDKDKSEAVSAVKGLS
                     ELNFELYATLGTHNFLEKHGISTQLVAKHSEKDGNQGAISAVDLINQGKIALVINTPF
                     GRGAARDGRKIRTAAVARGVSCITTLPGLKAAVEGIKELRRGKLTARSIQDWHAS"
     gene            complement(21838..22950)
                     /gene="carA"
                     /locus_tag="PLW53_03975"
     CDS             complement(21838..22950)
                     /gene="carA"
                     /locus_tag="PLW53_03975"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRD36613.1"
                     /translation="MAAYLVLDDGRIFEGESWACKGETFGEAVFSTGMTGYQETLTDP
                     SYHRQVVIMTAPHIGNTGMNTQDEESRKIWVSGFVVRNPSPRVSNWRSERSLEDDLIS
                     NGVVGIKGIDTRALTLHLRERGAMRVGIFSGSELSREEMVVKVRSAPEMAGAFLAEEV
                     TVDKVEIIPAVGERKFKVAAIDLGIKSATPRNMAARGIEVHLMPATITFEDVKAGGFD
                     GLFLSNGPGDPATMNSIVALVRSALDASLPIFGICFGHQILGRALGFDTYKLRFGHRG
                     INQPVLNVESNRVEITAQNHGFAVEAPLDGIFSTVYGPGMVTHICLNDSVVEGLKLLD
                     KPAFSVQYHPEAAAGPHDANYLFDQFLELLGKHASR"
     gene            complement(22951..24225)
                     /locus_tag="PLW53_03980"
     CDS             complement(22951..24225)
                     /locus_tag="PLW53_03980"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRD36614.1"
                     /translation="MNSYRIKGAKDSSGKVGNYFVSDGEFVNESELSSSAKEIDAKGL
                     TILPGFVDLHTHLREPGREDAETVLTGSMAAATGGFTAISAMPNTLPVADTAGVVEQV
                     YRLGKHHGICDVYPIGAVTVAQGGTQLAELSAMNQSEAGVRIFSDDGHCVSDALVMRR
                     ALEYVKTFDGIIAQHAQDPRLTQDSQMNEGEISARLGLKGWPAVAEEAIIARDVLLAE
                     HVDSRLHICHVTTAGGVEIIRTAKKRGIKVTAEVTPHHLLLTDDLAESYDPVYKVNPP
                     LRTKDDVQALREGLADGTIDIIATDHAPHPHESKDCEWSAAAFGMVGLETAFSIAYLA
                     MVETKLLTLTQLVDRMSKIPAQIGRYQGHGSISVGAGANFTLVDLDSKWKVERLKLAS
                     KSKNTPFDGMVLPGVITKTFHNGALVYERNSK"
     gene            complement(24222..25184)
                     /locus_tag="PLW53_03985"
     CDS             complement(24222..25184)
                     /locus_tag="PLW53_03985"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRD36615.1"
                     /translation="MKHLLSISDLTKDEAIEILDTAAELARLSEGAVKKFPTLRGRTV
                     VNLFFEDSTRTRISFEAAAKRLSADVINFAAKGSSVSKGESLKDTALTLQAMGADAVV
                     IRHGASGAAHQLAKQEWMKGVVINAGDGTHEHPTQALLDAFTIRRHLKSNQGDLSGVK
                     VGIIGDILHSRVARSNILLLKKLGAEVVLIAPPTLIPVGVFDWQVGVSYSLDDEIKSL
                     DVVMALRVQKERMDDLYFPTEREYARGYGLTSERMRQMKDKAIVMHPGPMNRGLEISA
                     ESADSQRSVIVEQVANGVSVRMAVLYLLLGGATFKELASSGESL"
     gene            complement(25181..25714)
                     /gene="pyrR"
                     /locus_tag="PLW53_03990"
     CDS             complement(25181..25714)
                     /gene="pyrR"
                     /locus_tag="PLW53_03990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRD36616.1"
                     /translation="MSVILNSGEISRALRRISHEILEKNHGPENLLLLGIPTRGAYLS
                     RRIASAISEIEKTELESGTLDITMYRDDLRLKAPRTLLPTSIPGGSIDGKDVVLIDDV
                     FYSGRTIRAALDALNELGRPKSVQLAVLIDRGHRELPIRADFVGKNIPTAKEESVRVH
                     LSEIDPLDEVIIEGRTQ"
     gene            25843..26328
                     /locus_tag="PLW53_03995"
     CDS             25843..26328
                     /locus_tag="PLW53_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD36617.1"
                     /translation="MTTPLEISKRLRQIRKQQNLTLKQVEIRSRGKWKAVVIGSYERG
                     NRSLSISKAKSLCEFYGVPLSALFQSEGNQQVPPSEGALRIDLRNLRSRLAENDPLIN
                     QLHNLLSFIARRRDDWNGEIMSIRLQDNEVLGLLVQKEPYELRKALEIRDLLFKAQGR
                     A"
     gene            complement(26306..26716)
                     /gene="nusB"
                     /locus_tag="PLW53_04000"
     CDS             complement(26306..26716)
                     /gene="nusB"
                     /locus_tag="PLW53_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD36618.1"
                     /translation="MSSRSKARKAALDLLYESDIRKSSAADLLLKRVTEMEYEVREFT
                     KELIEGIELNKRKIDELIATYAQGWDMDRMPVLDRNILRLSIFELLWSKSVPEAVAIS
                     EALELATNFSTEDSGKYINGVLSKVLEIKPDLVL"
     gene            complement(26717..27277)
                     /gene="efp"
                     /locus_tag="PLW53_04005"
     CDS             complement(26717..27277)
                     /gene="efp"
                     /locus_tag="PLW53_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007056944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRD36619.1"
                     /translation="MATTNDLKNGMTLDIEGQLWSVVEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVEKTFNAGVKVDVAILEKRDMQFLYKDQAGFVFMDSSTYDQMTISAETVGDAA
                     NYMLENTEAIVAVHEGNPLYVELPANVPLKITYTEPGLQGDRSSAGSKPATLETGITI
                     QVPLFIKQDEVVLVDTRNGDYLGRAN"
     gene            complement(27304..27744)
                     /gene="aroQ"
                     /locus_tag="PLW53_04010"
     CDS             complement(27304..27744)
                     /gene="aroQ"
                     /locus_tag="PLW53_04010"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRD36620.1"
                     /translation="MSMTKVLVLNGPNLSRLDLREKSYYGDISYSDIEQICRQGAKEF
                     NFDVEIKQSNDESELIGWLHAAADSKTPVIFNPAAFTHYSYAIRDAVVMLKAPCIEVH
                     LSNPLSREEFRHTSVIAGVVQGSIAGFGLDSYRLAFAALSKLVK"
     gene            complement(27745..28773)
                     /gene="aroB"
                     /locus_tag="PLW53_04015"
     CDS             complement(27745..28773)
                     /gene="aroB"
                     /locus_tag="PLW53_04015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRD36621.1"
                     /translation="MKPITVDAEKRYQISFTEDVWRDLSSLSQNRDVVLITPKSISVS
                     RGAAFASQYLLIEVEEGEGQKSIQSFNQVIEKIAAAKLDRNCLIVGLGGGATTDLAGF
                     VAASYLRGVDWIAIPTSLAGMVDAAIGGKTGLNLDAGKNLIGAFHSPSAVLIDQTFLN
                     SLSERDLKAGMAEVAKCGFIDDTEILDLIEANWKKNLTELIYRSIAVKAKVVSDDFRE
                     SSLREVLNYGHTLGHAIEKHSKYSLRHGEAIALGLRFAAQLSFEFSGLSAKDLDRQNQ
                     VLDTLGLNAKYPKDSWPELLEIMRNDKKVRGGRIRFVTLSALGTPTRAEGITDKQLEK
                     SFLESIGE"
     gene            complement(28770..>28996)
                     /locus_tag="PLW53_04020"
     CDS             complement(28770..>28996)
                     /locus_tag="PLW53_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRD36622.1"
                     /translation="MANVVFLDVSISNAAPRVGFNRDRPLLLGNPRQQWIALMEKRRS
                     TYEALAKTRVSTDNKKPVEVVEEIVKELAL"
BASE COUNT         7745 a         6880 c         6021 g         8250 t
ORIGIN      
        1 tcgctcaata aaaaagttga tgcgatagta ggaattgaga gtcgtggttt tattcttggt
       61 ggggcaattg ctaaagctct agagatcggt tttgtcacgg tgagaaaacc aggcaagcta
      121 ccaagagatg tctatcgaaa agattatgcg ctggaatatg gctttgatgc attagagctt
      181 cataaagatc ttcttgatcc atcagataac gtgataatcg ttgatgacgt gttagcaact
      241 ggaggcacag ctcttgcctg ccatgatcta gttgaaataa ctggcgctaa agtagttgcg
      301 catctattta tgcttgagat tgaatttctg caaggacgca agatgattag cgatacccat
      361 agcgaggttg gaataatctc gctccttaag gcataggtat tgggagcgag aaaatggcag
      421 agctaattta ttactgcgga accatggata gtggaaaatc aacgctagct cttcaaaccg
      481 cgcataatca tcaaagccgc gggcgagctg gtttgctctt tactaatcgc gatcgagcgg
      541 gaagcggcaa gatttcatcc cgccttgggc tgacttcaga ggcgctagaa gtatcacttg
      601 agatggatct ccataagttt gtagttgatc aactttcaac gggaagacga attgattatg
      661 tcatctgcga tgaagcccag ttctacgctc ctgaacaggt tgaacaatta gcccgaattg
      721 cagatggtct tggtatcgag gtttatgcct ttgggattct cactgatttt cgaaccaaac
      781 tattccctgg ttctgccagg ttaattgagt tagccgaccg ggttcaaacc ctgcaagttg
      841 aagccctctg ttggtgtggc aggcgcgcca ctcacaatgc tagaactatt ggcggggtaa
      901 tggttacaga aggtgagcag gtagttgtgg gcgatacaaa tgctgcaaat gaagttgctt
      961 atgaagtttt atgtcgacgt caccatatga ggaaagtgac tgcaagaaac tcgggcgcag
     1021 ctcatcaatc aagtgaccca cttccattta attaggagag gcaaaagaaa tgacatcaac
     1081 tagaggttat gccgctatgt cagcgaaggc agatttaacg ccttacgcct ttgaaagacg
     1141 agtcttaaga agtgatgatg ttggatttcg tattgatttc gccggtatct gtcactcaga
     1201 tattcatcaa gtgagagaag aatggggaaa ggcaattttt cctatggttc cgggccatga
     1261 aattgttgga acagtgacct cacttggatc atctgcctca agattcaaaa ttggtgatcg
     1321 tattggcgtt ggtgttttca tcgattcctg tagagaatgc aagaactgtc tcaatggttt
     1381 acagcagtat tgcttggagg gaatgactgg tacctacaat ggttatgaaa gaaacagcaa
     1441 agaaattgca cttggtggct attcaaacta tttcgtaatt cctgaaagct atgcgtatag
     1501 attgcctgac aatcttgata tggccgcaat ggctccgcta ctctgcgccg gaataacgct
     1561 ctactccccg attaagaaat ggaaagttgg tccaggaagc agcgtcgcag ttgctggtct
     1621 tggaggtttg ggacacatgg gagtgaaatt tgcagctgct cttggagcca aagtgactgt
     1681 gctctctcac tctgattcta aagaaagtga tgcaaaagcc tttggtgcaa ctgactttgt
     1741 aaacacaagc aaagccgatt ggcatcttgg atatcgcaag agctttgatc tgattttaaa
     1801 tacggtttca gctcaaatcg atctggagcc ctacctatcg acacttgcca ccgatggttc
     1861 gctggttgta attggccttc ctggcgcccc atatagcgtc agcgccggtt ctctgcttga
     1921 tggcaggcgt tctgttgcag gatcaatgat tggcggaatc cctgaaactc aagaaatgct
     1981 tgatttctgt ggagccaaaa atattgtcag cgaagttgag gtgattagcg ccgactatat
     2041 taataaggct tatgaaagaa ccattgcaag cgatgttaaa taccgctttg tgattgattg
     2101 ttcgactttc taaatattgc tcgcaattag tgcaaagagc ggtgcaagag ctaaagctgg
     2161 cagaaggtta cctatcgcta tctgcttgat attgagaagt ttcaaggcaa tacctaaaag
     2221 caatacacca ccagttgccg tcatagcgct gacttgatac ggctcaagaa ttgcgccaag
     2281 tgcaaatcca accccggtcc aagcaaattg atagatacca acaggcaagg ctgaaagagc
     2341 tactccccaa ccaagggcgg ctgcaaaggc aatagatgtg aaaaagtcga gaatactttt
     2401 caacgttagc tgctcaatgc ctgtagacat tccatcgcta atgcttccca gaatggcaag
     2461 aggaccaatt acaaagagaa gtgaagcgct gacgaaacct tcgacaaaag gagatttccc
     2521 atctttatca aatcgcgcct ttaatttgat tccaattact tcaagatgat cttcaatctt
     2581 tagcagagaa ccgagtaatc cacccacaag aagcgaggca agtacaacga gaagcggcga
     2641 accatctggt aaatatctat tgaaatcttc atcccaaaga gctttgattg cccctgccgc
     2701 agcaattaag gtgatagcgc caagcacatc cataacaaga tttcgcgtct tttcgctcat
     2761 cttatttcca agtaaaacac ctagcgcagc tcctgcaatg atcgccaata tattaataag
     2821 tgttccgact ccaacaaaca tgaagataag tctagagtat taatggtgta gcgttagaaa
     2881 gtgaagagat ttatagatgc gctaaggcct cataaaacca ttccaataac cccttggagc
     2941 gcttcgcatc gctgggatct ctctatttct agagttttga ttctcacttt tggtcttttt
     3001 atttttggaa tcggcgatag cttaataatt atttcagagc tcggtaatgc gccttggtca
     3061 gttcttgctc agggagttgc aacaagaagt gatatcacca taggaagtgc aacctttcta
     3121 attagttgcc ttgttctcct gctctggatt ccattaaaag agaaacccgg ctttggaaca
     3181 cttgcaaata ttgttgttat cgctattgga attgatgttg gaatagcttt tattgggact
     3241 cctgatttat ttatcattca acttctctat gtatttattg gcattggact aattggaatc
     3301 ggatcagcgc tctacatcac ctgcgcccta ggtcctggtc cccgcgatgg ctggatgact
     3361 tcccttcata gaaggacagg aatcccagtc ggtcgggtta gaactgcgat tgaggtttca
     3421 gttctccttg ctggttgggc tttaggcggg cgaatcgggc taggaactgc gctctttgcc
     3481 ttgctaattg ggtatgcagt cgctgccggc tttggtgtcg tttcccgact tactaagcag
     3541 taactaatag atttaagcca ctcccctcgg cttgtggact gcatccacac ggtgtcgcaa
     3601 ccaccaccgt taaaaaatta gcgacatccc ttcattggga tgaaaaggaa gagcatgctc
     3661 gattcatact tcaaaatctc acaacggggc tcatcagtag cccaggaagt ccgcggcggt
     3721 gtagttacct tctttacgat ggcctatatc gttgctctca atccactaat catcggtctt
     3781 gcaaaagacg gcgatggaaa attccttggt ggcggagatg ttccaaacct agcgctcgtt
     3841 gcagcagcaa cggcgttaat tgctggtgtc atgtcaattc tgatgggagt tgttgccaac
     3901 ttccctcttg caattgcaac tggcctcggc ttaaacacct tcgtagctgt tggtattgcc
     3961 tccaagatga cctgggcaga tgccatgggt cttgttgtcc ttgaaggaat tatcatcacc
     4021 gttctcgttc ttactggctt tagaactgcc gtatttcgcg cagttccaac tcagctaaag
     4081 atcgcgatct ctgtcggtat cggtctattt atcgccctta ttggtctagt cgatgccgga
     4141 ttcgtccgtc gcactggctc aggaccagtt ccagtaacac ttggaaataa tggtgaatta
     4201 gttggctggc ctgtaatcgt ctttgctttt ggtctcttcc taactatcgg tttgatggtc
     4261 agaaaggtca aagctgctct tctacttgga attattattt caacaggact tgcaatcgct
     4321 cttgagagcg cctttaagat tgggccactc tttgatggcg caactggcaa tgtaaatcca
     4381 aagggatgga atctaaatgt tccagctctg ccagaagctg tcgttgcaac tccagacttt
     4441 ggtctacttg gttcatttaa cctatttggt tcatttgatc gagttccact aatcacagct
     4501 cttctccttg tcttcactct ccttctagct gacttcttcg acactatggg aacgatgaca
     4561 gctatcggac aagaggcagg attaaacgat aaggatggaa ctcctccaaa tgcagatcgc
     4621 atccttcttg ttgactcact agcagctgtt gcaggtggcg ctggtggagt ttcttcaaat
     4681 acttcatata tcgaatcagc atcaggcgtt ggtgaaggcg ctagaaccgg acttgcttca
     4741 gtagtaactg gagttctctt ccttctaact accttcctag ctccattagt tgcggtaatt
     4801 ccttatgaag cagcaactcc agctctgatc gtcgtaggtt tcttgatgat gactcagatc
     4861 aaatcaattg attgggaaga ttatggaatt gcaattccag ccttcttaac catcattttg
     4921 atgccattta cctacaacat ctcggtaggt attggagccg gatttgtttc atatgtagta
     4981 attagatact tgcagggcag acgtaaggat gttcatcctc tacttcttct caccgcaggt
     5041 ctatttatga tctacttcct aaataaccca attaacgttt gggtttctta atcaagtagt
     5101 tgcaattaaa gatctggccc gggtattccc gggccagatt tatttcacca gataattgca
     5161 gtttttcctt tgttttccag gagttgattc actctagaaa agggcttaga tccaagaaaa
     5221 cctcgatggg ctgaaagcgg gctcggatga accccttcga ctaaacagtc cggcttaaaa
     5281 agatgggcta gttctctagc gcttgatccc catagcaggc caactgctcc caagttggcg
     5341 cagtgcgaaa caattttttc tgtgatttct tgccagccta gctttgaatg tgaagcgctc
     5401 tcacctgcgg taacagtaag tactcgattt agcagcatta ctccttgact tgcccattta
     5461 gtcagatcgc catctgcagt agttgttgaa ccaatatcag tcatgatctc tttttggata
     5521 tttaaaagcg atcctggaag cccagtcttt ctaggtggaa ccgaaaagga gaggccaatc
     5581 gcatctgaaa tatttggata tggatcttgc ccaacgataa ttaccttaac ttgttcaggc
     5641 ttaagctcca gtgcgcggaa tagatgcttc ttatctggca gtattcgctc gccttgatca
     5701 gctcgttttt gtagcttcaa gccaagctca tcgatctgcg agttatagtc ttggaggact
     5761 tccttccaat gatctggaac taaagaagaa agcattaatc ttttgccaca gcaaaataac
     5821 gcaaaccatt aaaagataat gaaaccctga cgccaaaaac ctcttcgata tttgcctcgg
     5881 taagagtttc agatatcgct ccggcgtaga gcgcttgacc ttctttaagt aagagcgcgt
     5941 gcgtggtgcc tgccggtatc tcctcaatat gatgagtgac aataactgtt gccggggcgc
     6001 gctcctcgct ggtgtatgag gtgatgcgag agagaatatc ttcacggcct ccaagatcta
     6061 atccagcagc tggttcatca agtaatagaa gttcaggatc tgccatcagc gctcttgaaa
     6121 tttgaactcg tttcttctca ccttcactta aagttgaaag agtcctctct cctagttctc
     6181 gcactccaaa gatatttaga agcgccttag cccttgattc atcccagaga tcgtaatcct
     6241 cttgccatct acctgcgatt ccataagctg cagttaatac aacgtctata actttctcat
     6301 ctcctgggag aaattccatc atcgcgctag aggtaaatcc tattcgcggt cttaactcaa
     6361 agacatcagt tgatcccatc ttctcgccaa gaatctccac actgcctttg cttgggtgaa
     6421 ttaaggccgc aagtaattgc agaagagttg tcttgcctgc tccatttggg ccaaggatga
     6481 cccatctttc ttgcgcattt atctgccaag aaatgggtcc taaaatgcgc ttgctagatc
     6541 taagaacttc cacctctacg cagcgcaata agggttccat agggcgacaa gatacctcgt
     6601 tgctattgag tattcgctaa acttcggcag tgaaagcgcg ggcaattatt ctcataagtg
     6661 gagcggcgag ccaagccccc aatcaattgg tggatgaaac cttaaaaccc ttcactctaa
     6721 gcattctgga tcgacaagag atacttatgg ccggtcgaat gattttggct cttgaaattg
     6781 agctagatcg cgctcatcta gatgctgttg agtcagattt aataaaggct ctagatcaat
     6841 tcgatgttgc atgtgaattg ctatgaaata cagattgata ctctttgatg tcgattcaac
     6901 tctaattaag caagaggtta ttgacctact tgctgagaaa tctgggcatg gcagagaggt
     6961 tcagcaaatc acggcaaggg ctatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnncttgg agtgagctat atgggcaaag agatactaag acagaaagct gacctgcatc
     7141 tagaaaaggc tggtcttaac gcccttatcc aatacctaaa ttagtaacga caatctagct
     7201 agcaattatt ggcaagcgtg cgcagttgct caaccatact tccagggttt tctgatttat
     7261 aaacagcgga acctgctaca aaagtatctg cacctgcccg gcgtgcagtt tcaatagtgg
     7321 ataacgagat gccaccatcg atttgaagcc agacatcatt taacttgcgc tgctctaaat
     7381 agcgacgtgc tttctcaacc ttaggcatca atgaggccat aaaagactga ccgccaaaac
     7441 ctggctcaac tgtcatcaca acgatcatat ctattgctgg cagaagctct tcaactacct
     7501 cgattggtgt atcaggtttg attgcaaggc cagccctctt accaaggctt ctgatgtttt
     7561 taagtgtttg cagcggatct tggcaagctt caaaatgaac cgtcatactt acggcatttt
     7621 ttgaggaata ttgacttacc ttctcatcta cttctgccac catgagatgc acatctattg
     7681 gcagcataga aaaatcaatt atttcccggg ttcgctcagg cgaaaaagta aaattaggaa
     7741 cgaaaacatt atccattaca tctagatgga ttagatctgc cgtagaagct accttttcaa
     7801 tttcccctgg aaggtcatca aaattggcat ttagaatgct aggacaaatt cttatcatcc
     7861 aacactctcc ttactcctta atagcgaaag gaacatagca tcagtgccat cgcggtgtgg
     7921 ccataattgt aaatcaccat caatatctgc ccgttcatct tgaaccttaa ttcttgtgaa
     7981 atcattatgc tgtttaagaa aagatctaat ctggaactta gtctcagctt ggtgggacga
     8041 gcaagtgaca taggcaatga tgccaccggg tgagagcaag cgtgtcgcag actctaaaag
     8101 ttcactttgc aattgagtga gattctttag atcttgaaga cttcgtctcc atctaacctc
     8161 tggtcgtctt ctaagtgctc caatgccagt gcaaggcgcg tcaagcagta ttcgatcaaa
     8221 ctttccaagc tcatctggca tggatcgacc atcagagaca ttaacttcgc catggtgcat
     8281 cacttgagag actaattttg ctcgttcatt tgaaatctcg ttcgcaagca aggatccccc
     8341 atctcgcttc aataacgctg atagatatgc cgctttcccg cccggccccg cacatagatc
     8401 tagccaccgc agatttggct taaattcatc gtaaaacttc tcaacaacaa gctgggaacc
     8461 tagatcctgg actccagcgc gtctctctct aatggctgca atgtctccag gatttccatc
     8521 aaatgtcgca gcaacagaac ttgatgctat tgcaagcgca ccggcttcca gaagttctcc
     8581 ttgagtagcc aaacctggcc aagcaatcag tgtcggtttc gcagggttat tgtttgcttg
     8641 aagaaggctc attacgtcac ttgagttctt taaagaatca aaatatgagc ttacgatcca
     8701 ttcaggatgg gagtattcaa gtgcaaatct tgcgtattcc tgggttggtt taacaagctg
     8761 ctcaaaatcg agtgagtcaa ttttgcgcaa gattgcattt acaaatgagg ctgttgactt
     8821 tccaactacc ttctttgcta gctcgacact ttcataaata gcagcatggc ttggaactct
     8881 catctgtttt aattgatgag taccgagcct taaaacaatc agagcttttg gatctatttg
     8941 ttccaaagat ctatcgctag ccgctgatat aaagtgatcg tgcctgccct gcattcgaag
     9001 agtgccataa accaattctg tggcgaaggc gcgatcatta gcttcaagta ttgattcaga
     9061 taaagctttt ggaagaatga gattgctgta tgcgccttgg agccctacct ctgcaagcac
     9121 ttcaaacgct aagaggcgtg ggtcatcaat tcttctgggt ttactactca ttcaaaagca
     9181 caaggaagcg taattcttgc ccctctcaac caatcactac tactcatctc acgcttattt
     9241 tcaggtttca cacggataat ctcaagagaa ccagaaccag ttccaaccag tagaggattg
     9301 actgaaataa cttcgcctgc tgctccagcc gtctcggata ctcttgcttc aattatttca
     9361 attcgtgtgc catttaatga tgtgtaagca ccaggccagg gttgaaaggc cctgatctgc
     9421 ctatcaagca ttttcgaatc tcgattccaa tcaattctgc cttcgctctt taaaatcttc
     9481 ggtgcaagca ttactccctc agactcttgc gtcgtagcgg gttctcctgc tgcaatcatt
     9541 attagggcct gattaactgg ttgaatgcat agcgatgaca gatctttcaa aacttcatca
     9601 cttctagatc tatcattcaa tggataactc aaagttgagt aaatcggccc ggtatcaagg
     9661 ccttgttcta gtttgaaaac cgtaacacca ctctgctcat caccggccgc tatggctctc
     9721 tgaactggcg ctgctccgcg ccatcttgga agaagggaaa aatgaacatt gagccatcca
     9781 tgcttgggta tctgaagctc acttgtttta attaatcgac cataagcgag tgtgataacc
     9841 agctctactt gagttttttg gagaatatct gcaagctctt tattcgtact tggcttatag
     9901 caagttacac cgatctcctg gcaataagaa gcaaagtcat tactacttac tgactgaccc
     9961 cgcccacttg cacgatcagg tgatgagata ccaccaagga gctgatgatc agaattcttt
    10021 atatggttaa gaagatcctt tgcaaggggt gaagatgatg caacaagaat cttcatcaat
    10081 tactcatcaa ttaaagactc ttgccgaatg tggaatgtgg tgagagtcgg atttcaggag
    10141 cagaacctgc ctcgcttgca gcaagaaacc attcagcctg tctaatctcc ttcatcgctt
    10201 ctttgcgaag ttctggtggt agtcgatcaa tgaatagaac tccatttaaa tggtctgttt
    10261 catgttgcag cgcccttgct agaagttctg ttccctcaac gatgagtggt tcgccatgca
    10321 tatcaaaacc ctttgcaacc gctcgcattg ctcttggagt ttggtaggaa agttcaggga
    10381 aagagagaca accctcatcc ccttcctgaa tctcatcgcc aagatctaga gttgggttca
    10441 ctaaatggcc aaagacatca tcaacatgcc agacgaaaac tcttaatgga acgcctattt
    10501 gcggtgctgc taacccagct cctggagcat caatcattgt ctctgttaag tcttttacta
    10561 agcgttgtaa ttccttatca aaatctatta cttcagtagc cggagtcgtt aataccgggt
    10621 ctccaaaaaa tctgatcggt tggattgcca tggcctaatt ctaacttact gaactaagaa
    10681 ttagatcttg tatggatcta cacgaatgga aaagagatct tgacctgatg ctgaacgata
    10741 cttctgcaga gcttttagtg agactaaaag agcattagcc ccttggtgag atatcttgag
    10801 atgaattgta cgattatcag cagatgtcag aatctccagg ctatcgaatt gctccaaaag
    10861 ctttcttcta agaccagaaa tcgctcgctc ctcaccggta acctgaatta ttcgcaccga
    10921 aggaggaagc gaaactgcct ctcgctcgct actttcagct cgaatcgctc gcattacgtt
    10981 atcgctaatc aacgcttggg aaagaggatg ggaagagggt agagaaagat aaataaatcc
    11041 cgaggagcgc actttcgctg ccaatttaaa ccatctaaag cgaagctcct cttctgcgcg
    11101 tataaaagct ctattgagaa gcgcttcacc attgagaagt actgctcctg cataaccagc
    11161 actgacttct ggctctacgc caaaagttga aaccacaatc gctggagagc catccagagc
    11221 tgcgattgct ttatcaccgg tagatgtgaa gattgcaact cttggaaaag cttttcccag
    11281 ttcttcaacg attttttctg ccccaccttt aacgatgcgc acttgatctg cttgacagat
    11341 ctgacagcgc cattgatcgc tttgaaaatc acatattgaa catgagtagt tatttttctt
    11401 gccaatgatc aatcttcctc cgcacttgca gagagcaaga tttcgacatc tctgacagga
    11461 gaaagtattt gcataacctt tatctgtcac gctaactagg actggtccta tcttgagcgc
    11521 ttttcgaatt gtttcgtgat aggagtttgg ttctgtggca atgtttgctc tgtgaccact
    11581 aagaaatttc cgctccgaaa tgcttgagat ccaacctatt tcaactaatc gtgctaattc
    11641 gagtgaaatc gagtgcgaaa cgaaactaat gcttgcatgc tcatgttctg atcgcaaaag
    11701 agccatatct ctcacgttcc agtagggcgc cttctgttct tgataatgtt ctactgactc
    11761 atcagcaatt actatctcat caagatgaaa cataggagca agaatggcgc ttctggtccc
    11821 tataactatt gcaggctttg ctgaagccgc taactcgaaa ttcttccttc tctgagaaga
    11881 ggtgagatgt gaaccaattt ctatgacctc ttgcttcatc tcccccaggg ctttggcaac
    11941 cttttctatt tctttctgtg ttggaagaat taaaagcaga ttcaaaccct tgcgctcttt
    12001 aacaattctc acaactcgag attcaattga ttctgagcga ttcaagagaa aaagacgcct
    12061 tgttggcttt gagccacttg tttgcttaat cagagggcta cccaacgagg gacttatcgc
    12121 ttttgtaaag gttggaagca ctgcagcaaa tattgatgct tgattagcga tatagcgctc
    12181 cgagactttc ttagtaaatt ctagaaaacc tggagtgaga cggactgacg ttaacaggct
    12241 atcaactggc ttaagtgctg cttggctttt gtttttgact gaaagcacaa ctccattttt
    12301 caaatcattc ttaaacggaa ccctcacaag ggaaccgacg tcaactagtt cctttagttc
    12361 ctctggaaga aagtaggaga agggttgttc taagtgaaaa actcctgtgt caacaagaac
    12421 ttcaacttgc aaactatcca aactttggcg cgttcttatt acagatttcc ctttttggcg
    12481 acgcagggtg agtggcttat ctgtggccac ggcaaacctt aacctttaac agctgcagct
    12541 agcgcgcttg ctttatcagt cttctcccaa gtgaaatcag ctaactcacg gccaaagtgt
    12601 ccgtatgcgc tagtttttga ataaattggg cgaagaagat ttagatctct aataatcgca
    12661 gctggtctta aatcaaaaac ttttaaaagt gcatctgata tttgctgaac tggaattttc
    12721 tcggttccaa atgtctcgac aaataggcct accggttgtg cttttccaat tgcataggca
    12781 acttgcactt cgcagcgatc agcaagaccg gcggcaacta cattctttgc cacccaacgc
    12841 attgcataag cagctgagcg atcaactttt gaagggtctt tgccactaaa tgctcccccg
    12901 ccatgtcgag ccataccacc gtaggtatcc acaataatct ttcgtccagt tagtccggca
    12961 tcacccattg gtccaccgat aacaaagcgt ccagttggat taataaggat tcgcacatca
    13021 tttgctggta gatcaaaaga ggctaaaacc ggatcaataa caaacttctt aatctcagga
    13081 gccatcttct tctcaatatc tatttcgggt gcatgttgag acgaaatcac aactgtattc
    13141 agcgcaactg gcttatctcc gtcatattcg atggtcacct gagttttgcc atcgggacgc
    13201 aaatatccaa gttgaccact ttttctctca ctagagagac gctcagcaag tctatgagcc
    13261 atcgctatag gtagcggcat taactctttg gtatctcgag tggcataccc aaacatcaac
    13321 ccctgatctc cagctccttg taaatctagt ggatcctttg aagcggcgac tctttcttcg
    13381 aaggcgtgat caacgccgac tgcaatatct ggtgattgtt gcccaataga gattgatact
    13441 ccgcagctat ttccatcaaa gcctttttca gagctgtcat agccaatatt gagtactgtt
    13501 tcacgaacaa gtcccattac atcggcatag ccctcggtgg tcacttctcc ggcaacatgg
    13561 acttgtcctg ttgtgattaa agtttcacag gccactctac tttttggatc ttgagaaaga
    13621 agtgagtcaa gaacagcatc tgaaatagca tctgcaattt tatcgggatg gccctcagtt
    13681 actgattctg atgtaaaaag acgcttagcc attgatcttc tccaatttct ttgccatctg
    13741 aataactatg ctctttgcta cctcatattt actaacgcca ttaaaatcaa ttgaatcgac
    13801 acctaaccca ataagcgttc cactggtttg atcttgacca aatatagcgc caccagagac
    13861 atcgttaaca tagagaagat cagcgccctt gcttatcagc ttctctccac cagctctaat
    13921 taagtctttt gctgtttctg cagcaaatcc aacaactacc tgattacttc tcttactctg
    13981 agccacctta cttaaaatat ctggattagt ggtgagatta attgagtcaa aatcagcctt
    14041 tttaatcttc gttgaagaac tagatactgg tcttgcatcc gcaaccgctg ccgccattac
    14101 tagggcatca gcgctactaa attcactttc aactgctgac atcatctcta gcgcggtatt
    14161 cacctttata actcttactc catctaattc aaatggatcg gtatggcctg caatgacagt
    14221 tacctcagca ccgcttctca gggcttcata ggcaatagct agaccctgcc tgccacttga
    14281 tttgtttcca ataaatctga ctggatcaat ttcttctcta gtaccgccgg ctgttactag
    14341 gacccttttg ccttcaagca atagctctgt cttggtaaga cttagaactt gtgtgacgat
    14401 ctttgaactc tctggaaatc ttcccactcc aaaatcttta cccgataggc gaccaacttc
    14461 tggatccatc acataaaatc cgcgggagcg aagcgtttca acattttgta ccgttgccct
    14521 gttgagccac atgtttgggt gcatcgcagg aactattagt atcggtgcat ctgatgcaag
    14581 gactgttgtt gttagtaaat catccgcgcg tccttgggca agctttgcaa tagtgtcagc
    14641 agttgctgga gcgattacga ttagatctgc gctatcggcg gctgctaggt gtgaaacttc
    14701 tgcgggattt tcccaaacgg tacttaaagc aggctgtcct gagagggctt cccaagttgc
    14761 tcgacctaca aaattaagac ttgattgggt tggaataact cgtactagaa aaccagcatc
    14821 ttgcagacgg cgaagcagat cacaagcttt ataggcagct attccgccag aaacgccaag
    14881 aacaatttga cgacctttgg ggaagattat cttttcgcca ctcatggctc ttcttaactc
    14941 tgtggttcta gattctcaat tgttaaaagg ccctcattga tctcgcgcat agcaattgat
    15001 agaggttttt catgaacgtg agtttcaaca agtggaccaa catattcaag gagaccttca
    15061 ccaagttgtg agtagtaagc attgatctga cgcgctctct tagctgcgta tagaactagt
    15121 gaatatttag taccgctctt atccaataat tcatcgatcg gtggatttgt gatgccatct
    15181 aatgtcgcca cagctactcc ccttttaaag gtttgtcctt aaggtttact ttcaatactt
    15241 ctatgaagtg gccaaggata gcagtgcttc aagcacatct tcgactcgat gattgaccag
    15301 gatatgctca aattccttcg ctgccgccat ctcgctacgc gcaagatcta ggcgggctgc
    15361 aatcctctca ggtgagtcag tgcctcgatt aacgatgcgc gcttcaagct cttgaaaaga
    15421 gggtggctgg agaaagacca aagtagcctc tggcatagca gcttttactt gacgagctcc
    15481 ttggatttca atctcgagca aaacattctt cccctctgat aacgcttgat caataggggc
    15541 tttaatactt ccatatctag ctccagcaaa ctctgcccat tcaagaagta gattcttaga
    15601 tatcaattca tcaaagcttt tatcgtctat aaaatgatag gagcgtccat caacttcgcc
    15661 cgctcgtggt tttctcgtag tagctgaaac agaaaagaag aaattcggat tatcaattag
    15721 ctttgaaacg atagtgcttt tgccgacacc tccaggccca gagagaacaa ttaatttgcc
    15781 tcgcttactc attagttggc cttcattaat tcaagaagtt tttcatattg gcttcgccca
    15841 acaccaccta ctcgtcgagt tggcgagatt ccagcctttt caagaatatt cttggcacga
    15901 acttcgccca ccccaggaag agattcaatt aactctcgaa ctcgcatctt cttgattgat
    15961 tcatcttgcg cactgaaagc atccttccaa cttttcttac caactttgat ctcttctttg
    16021 aaagcagcgc gggttctcct tgctgcaaca gccgctttaa gcgcatcacg tcgctcttgc
    16081 gtggttaagt ttggaggagc gcccatggct taattaaacc aagttatcta agcgaagccg
    16141 taatcgcctc tgctgcttgc tttggatcag aggcctttgt aataggacgc cctatgacaa
    16201 caaaatctgc gccagcctct atcgcctccc ttggggtcat aatccgttct tgatcatcac
    16261 gctctgcgcc agatggtctt actcctggtg taattaaagt taccttctct cccaaatgct
    16321 ttctaaggag cgagacttcc tgcggggagg agacaatggc tctggctcct gagttaacag
    16381 cgttttttgc aaggctaaga gttaaattct caatgttctc atttaacccc atagcaacca
    16441 tctgctcttt atctagtgag gtaagaatcg ttaccgccgt aatttcaacc tttggcaggg
    16501 ttgagctagc tgcgctaatc atctttgagc caccagaggc gtgcacagtt agaaactttg
    16561 gatttaaatc tgctactgat ctacatgcgc cagctacagt attaggaata tcgtgaagct
    16621 ttagatcaag aaatatctcg atatcagtaa atttatcttg aatctcattt acgccgcttt
    16681 tgccatgaga gagatagaac tctagaccta atttgtagat accgatgtgt tccttggtca
    16741 aattgatcat ctcaaccgta cttgccatat cgttaaaatc aagggcaagg attactggac
    16801 tgctatttct catgagctac acctatagca gcggcaacgg agttgaattt gcgttcttta
    16861 aggagatcta tcagctcatt cttaattttc attaccgctg ttggatctcc gaaatttgct
    16921 gttccgactg aaactgccga agctcccgcc ataatcaatt caagagcatc tcgacctgat
    16981 gcaaccccgc ccattcctaa gattggagta tttttaaaag cagcatgtac ttgatagata
    17041 gctctaactg ccacaggcct aatcgctggt cctgatagcc cgcctgtctt gccagcaagt
    17101 ttaggacgca tggcatcgat atcaattgcc attccgagca aagtgttaat gagtgctaat
    17161 ccatctgcgc ctgcacttat tacctctcca gcgatagaga caatatctgt gacatctggc
    17221 gagagcttta caatgattgg aagttctcca cctacatttc ttctaactga ttcaactact
    17281 gactttgaag ctactggatc gcaggcaaaa accatccctc gattctccac atttgggcag
    17341 gagatattga cttcaatacc gctaattccg gctactcccc ttaattttcg agcaagtgat
    17401 gcatactcct caatcgtttc accagagatt gaaactatga cgcgagcgct ttttgaaatt
    17461 agccaaggga tatcttcggc aaggaattga tcaataccag gtccttgtaa tccaattgag
    17521 ttaagcattc cacttggggt ttcagccatt cgaggagtag ctcgtcctga acgtggctta
    17581 actgaaattg atttagtgac taccgctcct agttcatcta aatcaaaaaa ctgagaaagc
    17641 tctttgcccg atccagcgca accacttgct gtgaagattg gtgatggaaa gaacttaccg
    17701 ccgaatttgc agcggggatc aaatgcttga aactggctat ccattaaaac cccatgtccc
    17761 ttgcggaact ttgcgcgagc tatcccaaat tacctttgat ccatccatca ctgggccatc
    17821 gatacaagat ctagtcatct ttattacccc agttgaatcc ttgacaggaa ggacgcaggt
    17881 catacagatt ccaattccgc aggccatcgc ctcttcaacc gcgcattggt gaagcaatcc
    17941 agctgtagtt gcaatctcat caattgcagc aagcattccc atcggaccgc aagaataaat
    18001 aatttcactt ccatgttttt caattaatcc tggaagcacc tgggtgactc gacctttaat
    18061 tcccgttccg ccatcttcgg ttgtaattgt tagcgaatta acgctgcgct ttccttcaag
    18121 aggagcataa atcttgccag ctgttgacgc gccgagaacc atatcaaccc ggcatccacg
    18181 ttctttgaga acttctgcga ggccaaaaag tggagcagag ccatatcccc cgccaactaa
    18241 gagagttgat gcaggctctg tgggtatgcc aaagctagtt ccaagtggcc caacaatatt
    18301 taccacatcg tgaatctcaa gacttgtgag ccagcgactt ccctggccat gcggagccac
    18361 aactaactca atagttccgc cgtattgacc acgatcactt gcgcgataaa ttgcaaaagc
    18421 cctgcgaaga atcattgaag atccagttcc acctacggag atagcaacaa aattgccagc
    18481 ccggcaatgg gccgccatat ccccagctgc aaagaccata tgatggtatg cgcccacctt
    18541 tttattggaa atgatttcag cgctgcgctg ttcagctcgc ttatttatag aactcatgaa
    18601 gcgtgccaat cttgaattga tctagcagtg agttttcctc gtctaagttc ctttattccc
    18661 tcaactgccg ctttcagccc aggaagggta gttatgcaag agactcccct agcaactgct
    18721 gcagttctaa tttttcgccc atctcgcgca gctccacgtc caaaaggtgt gttgatcaca
    18781 agggcaatct tgccttgatt aattagatct accgccgaaa tagctccctg atttccatct
    18841 ttctcgctat gttttgcaac gagctgcgtt gaaataccat gcttttctag gaaattatga
    18901 gttccaaggg ttgcatagag ctcaaaattt aactccgata accccttaac tgctgaaact
    18961 gcctcgctct tatctttatc tgagagtgag atgaagattg cgccactttg cggcagagct
    19021 ccaaacgctg ctatctggga ttttgcaaat gccgcgccaa aagttgaagc aattcccatc
    19081 acttcacccg tcgacttcat ctcgggacct aaaacagtat caacgccttc accatctacc
    19141 cttcggaacc gattaaaggg aagcactgcc tcctttacgc taatgccact tgcaacacca
    19201 tcaccaattt ctggaagcat cccactctct cttagctctg aaatcttgac tccagttgct
    19261 atcagtgccg ccgcttttgc tacctgcctg cccgttgcct tactgacaaa tggaacagtt
    19321 ctagatgctc tgggatttgc ctctaagaca taaagctttg attccgagat ggcgtattga
    19381 atattgatta gtcctaagac tccaatacca gaggcaagtt ttttagttgc ttcccgaatc
    19441 tcggcgatca attccttggt tatagattga gctggaatca cgcaggctga gtcgccggag
    19501 tgaaccccag cctcttcaac gtgctccata attcctgcca ggtaaagttc atttccgtcg
    19561 tatagcgcat cgacatctat ctcgattgca tcatctaaaa acttatcaat caagactgga
    19621 tgctcaggat ttatctgagc cgctatggtt atgaactcct ctaatgaagc atcatcataa
    19681 acaatttgca ttcctcgccc gccaagaaca aatgaagggc gaactagtac tggataccca
    19741 acttcgctcg caacctttaa agcatctgaa tatgaaaaac ctgtaccaaa tttaggcgct
    19801 ctcagtgagt tctcttctag cacttttcca aaagcgccac gatcttcagc taggtgaatt
    19861 gcctccggcg ttgttccaag aattggcacc cctgcgctct ttagctctgc tgcaattcca
    19921 agtggagtct ggccgcctaa ttgcacaatg actccggcaa tcggccctgc agcaagttct
    19981 gcgcggtaaa cctcaagaac atcttcaaca gttaatggtt cgaagtaaag gcgatctgcg
    20041 gtgtcataat cggtagaaac tgtctctgga ttgcaattaa tcattatggt ctcatagccc
    20101 gcctctgata aagcaaaaca tgcatgaacg catgagtaat caaattcaat accttgaccg
    20161 attcgattcg gtccgcttcc tagaatcaaa attgcaggtt tacttcgagg ttttacttcg
    20221 ctttctaaat cgtaagatga gtagtgatat ggagtaaagg cttcaaactc accagcgcag
    20281 gtatcaacgg ttttataaac cggaaacaaa cctagtctct ctcgcacttt caatatctct
    20341 gaagcgttag tgccgcgaag ctgggcgatc tgttgatctg aaaaacccat ctcttttgca
    20401 cttcttagta gttctagaga tagatcacct gcggctttaa tctctgaggc tttactgttt
    20461 atctcggcaa tttgcgagag aaaccaagga tcaattgcgc accttgcaaa gacttcttcg
    20521 attgatgccc ccagtgctag cgctctttga acttgaagca acctatcttg tgtcggtgtg
    20581 gcgattttcg ccatcaagtc ttcaaggctt tcggaaatat cccaagagaa agatgagccc
    20641 ttcttctcca agcttcgcat tgctttttga agcgcttcag gaaaagagcg accaaaggcc
    20701 atagcttctc caacgctctt catcgtcgtt gtcagacgtg tatcagcttc agggaatttt
    20761 tcaaaagcaa agcggggaat cttcaccacg atgtaatcaa gagttggctc aaatgaagct
    20821 ggcgttttct tggttatgtc atttgggatc tcattcagtg aatagccaat agcaagtttg
    20881 gttgcaattt tggcaatcgg gaaaccagtt gcctttgagg caagtgcgct gcttcttgaa
    20941 acccttgggt tcatttcaat cacaactacc cgaccattgg ctggatttat cgcgaactga
    21001 atattgcaac ccccggtggc cactcccacc tcgcgaatga ttgcaatgct taagtctcgt
    21061 agattttgga actcaacatc ggtaagagta agagctggag cgacagttat tgaatctcct
    21121 gtgtgcacac ccatcggatc gatattttca atgctgcaga caatgacaac attgtcatga
    21181 ttatcacgca taacttctaa ttcatactct ttccagccga taattgactc ttcgagtaga
    21241 acttcattaa cagggctaaa acgcaaacca gcgccagcaa tcaactctaa ttcatctctg
    21301 ctaaaagcaa ttccgcttcc aagtcccccc atggtaaatg aaggtctaac cacaactgga
    21361 aaacttagtt cactggcagc tttaaaacat tcatccatac tgtgacagat aattgactta
    21421 gcagactctc caccaatttt ggcaacgatt gccctaaatt cttctcgatt ctcaccgcgt
    21481 ctaatggctg ccacatctgc gccaattagc tttgtctggt atttttccaa tattccattc
    21541 tcatgaagcg ttatggcggc atttaacgca gtctgtccgc caagagttgc caagatcgca
    21601 tctgggcgct ccttagcaat aacttgctca agaaaatcca aagtaattgg ctcgatatag
    21661 gtggcatcgg caaactctgg atctgtcata attgttgcag gattcgaatt aaccagaata
    21721 actctgatgc cttctgcctt tagaacgcga caagcttgag ttccagaata atcaaattca
    21781 caggcctggc caataacaat tgggccgctt ccgattacca aaacactttt aatcgattta
    21841 tctagaggca tgtttaccta acaattcaag aaactgatcg aagagataat tggcatcgtg
    21901 aggaccggca gctgcctctg ggtgatattg aaccgagaag gctggtttat ctagaagctt
    21961 tagtccttca acaactgaat cattgaggca gatgtgagtc accattccag ggccataaac
    22021 tgttgaaaag attccatcta gtggggcttc aacggcaaaa ccatgattct gagcagtgat
    22081 ctccactcta ttactttcaa catttagcac tggttgatta atcccgcggt ggccaaatcg
    22141 aagcttatat gtgtcaaaac ctaacgctcg tccaagtatt tgatgtccaa aacagatacc
    22201 aaaaatggga agtgatgcat caagagcaga tcggaccagc gctactattg aattcatcgt
    22261 cgctggatct ccgggaccat ttgaaagaaa tagcccatca aaaccaccgg ctttaacatc
    22321 ttcaaaagtt atcgttgctg gcattaaatg cacttcgatt ccacgcgcag ccatatttcg
    22381 aggagttgcc gatttgattc caagatctat cgctgcaact ttaaattttc tctctccaac
    22441 tgccgggata atctcaactt tatccacagt aacctcttcg gcaagaaaag ctccagccat
    22501 ctccggcgca cttcttacct taacaaccat ctcttctcgc gacagttcgc tgcctgagaa
    22561 gatcccgact ctcatagcgc ctcgttcacg caaatgaaga gttagagctc ttgtatcaat
    22621 tcctttaatc cccacgactc catttgaaat taagtcatct tctaaacttc gctctgagcg
    22681 ccaattggaa actcttggcg aaggatttct caccacaaat ccacttaccc aaatttttct
    22741 tgattcttca tcttgggtat tcattccagt attgccaata tgtggggcgg tcatgattac
    22801 aacttggcgg tgataactcg gatcggtcag tgtttcttga tatcccgtca ttccagttga
    22861 gaaaactgct tcaccaaagg tctcgccctt gcaagcccaa gactctcctt caaagatacg
    22921 gccatcatct agaactagat aagccgccat ttactttgag ttcctttcat aaaccagcgc
    22981 gccattatgg aatgtcttag taataacccc cggcaagacc atgccatcaa aaggtgtgtt
    23041 tttgctctta gaggcaagct ttagtcgttc tactttccat tttgaatcaa gatcaacgag
    23101 tgtgaaatta gctcctgcgc ccaccgagat tgatccatgg ccttggtaac gtcctatctg
    23161 cgctggaatc tttgacatgc gatctactaa ttgagtaaga gtcaaaagtt ttgtttcaac
    23221 catggccaga tatgcaattg agaaagctgt ctcaagacca accattccaa atgctgcagc
    23281 gctccattcg caatcttttg attcgtgcgg atgaggcgca tgatctgttg caatgatgtc
    23341 aattgttcca tctgcaagtc cttcacggag tgcttgtaca tcatctttag ttcttagcgg
    23401 aggatttact ttatagaccg ggtcgtagct ctctgctaaa tcatcggtta aaagaagatg
    23461 atgtggggtc acttcagcag tgactttaat tccgcgtttt ttagcagttc taataatctc
    23521 aactccacct gcagttgtga cgtgacagat atggagacga gaatcaacat gctctgcaag
    23581 gagaacatct cgggcaataa ttgcttcttc ggcaacggcc ggccaacctt ttaaaccaag
    23641 gcgtgctgag atctcgcctt cattcatctg tgaatcttgg gtaagcctag gatcttgtgc
    23701 gtgctgagca ataattccat caaaggtctt aacgtattca agcgctcttc gcataaccag
    23761 agcatcagag acgcaatgtc cgtcatcgct gaagattcgc acccccgcct ctgattgatt
    23821 catggcagat agttctgcaa gctgcgttcc accttgagca acagtgaccg ctccgattgg
    23881 ataaacatca catattccat ggtgtttgcc taaacgataa acctgttcaa caactccagc
    23941 tgtatcagca accggcagag tgttaggcat tgcagatatt gctgtaaatc ctccagttgc
    24001 cgctgccatt gaaccggtca aaacagtttc ggcatcttcc ctacctggct ctcgcaggtg
    24061 agtgtgaaga tccacaaaac ccggcaagat cgttaaaccc ttggcatcaa tttctttagc
    24121 gctacttgat agttcacttt cgttgacgaa ttcgccatca gaaacgaagt aatttcctac
    24181 cttcccactt gaatctttag ctccttttat ccgataagaa ttcataaact ctccccgcta
    24241 ctagcaagct ctttaaaggt tgctcctccg agaaggagat atagaactgc cattcgaacg
    24301 ctaactccat tagcaacttg ctccacgatt accgagcgct gcgaatcagc ggattcagcc
    24361 gaaatctcaa ggcctctatt catcggacct ggatgcataa ctattgcctt atctttcatc
    24421 tgcctcatcc gctctgaggt aagaccatat cctctggcat attccctctc ggtgggaaaa
    24481 taaagatcgt ccattcgctc cttctgaact cgaagcgcca ttactacatc aagggattta
    24541 atctcatcat cgagtgaata gctgacccct acctgccaat caaagacgcc tacaggaatt
    24601 agagttggcg gagcgattaa aactacctct gcgccaagct tcttaagaag gagaatatta
    24661 cttctagcaa ctcttgaatg gaggatatct ccaatgattc ccactttaac tcctgataaa
    24721 tctccttgat tcgactttag atgacgacgg atagtgaatg catcgagaag agcttgggtt
    24781 ggatgttcat gtgttccatc gcctgcatta attaccactc ccttcatcca ctcttgctta
    24841 gctaattgat gggcagcacc tgatgcgcca tggcggataa cgactgcatc tgctcccatt
    24901 gcttgaagag taagggcagt atctttaaga ctttcgcctt tgctaacact tgatccctta
    24961 gcagcaaagt taattacatc agctgatagg cgcttagcag ctgcctcaaa tgaaattcta
    25021 gttctagttg aatcttcaaa gaaaaggttt accacagttc ttcctcgaag agttggaaat
    25081 ttcttcaccg ccccttcaga gagtcgggca agttcagccg cagtatctag aatctctatt
    25141 gcttcgtctt tagttaaatc tgatattgaa agtagatgtt tcattgggtt ctgccttcaa
    25201 tgattacttc atcaagtggg tcaatttcac ttaaatgcac cctgaccgat tcttcttttg
    25261 ctgtcggaat attctttcca acaaaatctg cccttattgg tagctctcta tggccacgat
    25321 cgataagaac ggcgagttgg acgctctttg gtcgacccag ttcatttaag gcatcaagag
    25381 cagcgcgaat cgttctacct gagtagaaga catcatcgat aaggacgaca tcttttccat
    25441 caatacttcc acctgggatg ctggttggaa ggagagttct tggagctttt aaacgtagat
    25501 cgtctcgata catcgttata tcgagtgtgc cgctttccaa ttcagttttt tcgatttcag
    25561 agatagcgct tgcaatacgt ctacttaaat atgctcctct ggttggaatg ccaaggagaa
    25621 gaagattttc tggaccatga ttcttttcta gaatttcatg agaaatgcgc cgtagcgcgc
    25681 gggagatttc accgctattt aaaataacgc tcattacttc tccttcgccg cctcacagga
    25741 cggatcgtta aaggttggcg aatactagca tgtgacggaa agggtcctgg aatctgtgga
    25801 aaaggcggag gggctctcta gctattggct tattttgacc caatgacaac acctttagag
    25861 atatcgaaaa gacttaggca aatcagaaag cagcagaact taaccctcaa acaagtagaa
    25921 atacgctcaa ggggtaaatg gaaagcagtt gtaatcggct cctatgagcg gggtaacagg
    25981 tcgctaagta tttcaaaagc aaaatccctc tgcgaatttt acggagttcc cctctctgct
    26041 ctctttcaaa gtgaaggcaa tcagcaggta cctccttctg aaggcgctct gcgaattgat
    26101 ctaaggaact taagaagcag acttgcagag aatgatcctt tgatcaatca gctccacaat
    26161 cttttatcct tcatcgccag aaggcgtgat gattggaatg gagaaatcat gagtattcgc
    26221 ctgcaagata atgaagttct aggactattg gttcaaaagg agccgtacga gctacgaaaa
    26281 gcgctagaaa taagagattt actctttaaa gcacaaggtc gggcttgatc tccaacactt
    26341 tgctcaatac tccgtttata tacttacctg aatcctcggt ggaaaagttt gttgcaagct
    26401 ctagagcctc gctgatagca actgcttcag gaactgactt agaccaaagt agctcaaaga
    26461 tagaaagacg taggatgttt ctatcaagaa ctggcatccg atccatgtcc caaccttgag
    26521 cgtaagttgc aattaactca tcaattttcc gtttatttaa ctcaattccc tcaattaatt
    26581 cttttgtaaa ttctctaacc tcatactcca tctcagtgac tcgtttgagg agtaagtcag
    26641 ctgcactact ttttctgata tcactttcat agagcagatc tagcgcggct tttctggcct
    26701 tagagcggct gctcacttaa tttgcgcgcc cgagataatc accgttgcgg gtatcgacca
    26761 agacaacttc atcttgctta ataaagagag gaacttgaat tgtgattcct gtttcaaggg
    26821 ttgccggttt agagcctgca gatgagcgat ctccctgtaa gcctggttcg gtgtaggtaa
    26881 tctttaacgg aacatttgct ggaagctcga catagagcgg atttccctcg tgaacagcaa
    26941 ctatcgcttc agtgttttca agcatgtaat ttgcagcatc tccaactgtc tcagcagaga
    27001 tggtcatttg gtcataggta ctggagtcca tgaagacaaa tccggcctga tctttataga
    27061 gaaattgcat gtctcgcttt tcaagaatag ctacatcaac tttaactccg gcattaaatg
    27121 tcttttcaac tacttttcct gaaagaacat tcttaagctt agttcgaacg aatgctggac
    27181 ccttacctgg tttaacgtgc tgaaattcaa caacgctcca gagctgacct tctatatcta
    27241 gggtcattcc attcttaaga tcattagtgg ttgccatagc ggcgaagatt accttttatg
    27301 cctctattta actaatttac tcaaggctgc aaaggccaag cgatatgaat caaggccaaa
    27361 gccagcgatg ctgccttgga ccactccagc aattaccgag gtgtggcgaa actcttctcg
    27421 agataacgga ttgcttaagt gaacttcaat gcaaggtgct ttaagcatca ctactgcgtc
    27481 acgaatcgca taagaatagt gcgtaaaagc cgcaggatta aagattaccg gcgttttact
    27541 atccgcagct gcgtggagcc agccgatcaa ctctgactca tcattactct gctttatttc
    27601 aacatcaaaa ttaaactctt tagctccttg acggcaaatc tgctcgatat cagagtaaga
    27661 gatatctccg taataagact tctcacgaag atctagtcga gaaagattag gcccattgag
    27721 cactaatact tttgtcatgc tcatctactc tccaatactt tctaggaaac tcttctctag
    27781 ctgcttatct gtaatacctt cagctcttgt tggtgttccc aaagcgctca aagtaacaaa
    27841 gcgaattcta cccccacgca ccttcttgtc attgcgcata atctcaagca gttctggcca
    27901 tgaatctttt gggtatttgg cattaagtcc cagggtatct agaacctgat tctgcctatc
    27961 aagatctttg gcggaaagcc cagagaattc aaacgatagt tgagccgcaa atcgcagtcc
    28021 tagagctatc gcttcaccat gacgaagaga gtatttcgaa tgtttctcaa ttgcatggcc
    28081 caaagtgtgg ccataattca aaacctcgcg aaggcttgat tccctaaagt cgtcactgac
    28141 aacttttgct ttaactgcga tagatctata aatgagctcg gttagattct ttttccagtt
    28201 tgcctctatt aaatcaagta tctcggtgtc atcaataaag ccacatttag ctacttctgc
    28261 cattcctgct ttgagatcac gttcagaaag tgaattaagg aaagtctgat caatcaaaac
    28321 agcgctgggt gagtgaaagg ccccgataag attttttcct gcatcaagat ttaacccggt
    28381 ttttcctcct attgctgcat caaccattcc ggcgagtgaa gttgggatgg cgatccaatc
    28441 aacaccgcgt agataggaag cggctacaaa acctgctaaa tcagtggttg ctccccctcc
    28501 taggccgaca atgaggcagt ttctatcaag tttggcagca gcgatctttt caattacctg
    28561 gttaaaagac tgaatactct tctgcccctc cccttcctcc acctcaatca agagatattg
    28621 cgatgcaaac gctgctcccc ggctgactga aatagattta ggagtaatga gcactacgtc
    28681 tcggttttgg gatagcgaag agagatctct ccaaacatcc tctgtaaagc taatctgata
    28741 tcgcttttct gcatcaacag ttattggctt catagcgcca actcttttac tatctcttca
    28801 acaacttcga caggtttctt gttgtcagtt gaaactcttg ttttggcaag tgcttcatag
    28861 gtcgaacggc gcttttccat caaggcaatc cactgctgtc tgggatttcc aagaagtaaa
    28921 gggcgatctc gattaaagcc tactcttggt gctgcgttcg agattgaaac atcaagaaaa
    28981 acaacatttg ccattg
//
LOCUS       DAOROD010000014        28478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_50_length_28478_cov_38.054111, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000014 DAOROD010000000
VERSION     DAOROD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 28478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28478
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_28478_cov_38.054111"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..639
                     /locus_tag="PLW53_04025"
     CDS             148..639
                     /locus_tag="PLW53_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD36623.1"
                     /translation="MSTEITVTELSSDDWPRLRDLRLAALADSPAILAGKLDEERNFT
                     EEQWRETFKKLSYVVASIDGKDVAMINVENLVGDFGATCWLGGLWSNPEYRGSGAVRA
                     IFNYMDAVASKRGWMVQGLGVMESNTSAIAVFEKYGFTKRGDRVESRGKPGSYYFRMI
                     KSL"
     gene            complement(634..891)
                     /locus_tag="PLW53_04030"
     CDS             complement(634..891)
                     /locus_tag="PLW53_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HRD36624.1"
                     /translation="MVKWAVIAAIITLALMIFSIIDCSRTSENNIRSLPKWAWLVIII
                     FVPAIGSIAWIIAGRPKGNGRGRRKRKIIPPDDNPDFLGKL"
     gene            complement(901..1770)
                     /locus_tag="PLW53_04035"
     CDS             complement(901..1770)
                     /locus_tag="PLW53_04035"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HRD36625.1"
                     /translation="MTTAEQWVQGARPKTLYAAIAPVLVGTAFAGYNASLLHFFLALI
                     VALFLQIGVNYANDYSDGIKGTDNDRVGPIRLVASGLAKPIRVRNAAFLSFVLAALAG
                     LLLSYRTSWALIPIGLISIAAAWGYTAGSKPYGYMGFGEVSVFIFFGVIATNGTYFVH
                     VEQLSSEVLLASFALGALSCAILTLNNLRDLPKDAQVGKRTLAVIIGDAQSRDLYRWL
                     IFFALVISAALSIFSPYYLIALISAPLAIKSVRSVKQGASGIELVDLLAKTGRIQIIY
                     AAALSLAALLVAR"
     gene            complement(1757..2806)
                     /locus_tag="PLW53_04040"
     CDS             complement(1757..2806)
                     /locus_tag="PLW53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD36626.1"
                     /translation="MSVMELAVKREVQIIEPAWSMPHFLEKLLLALKDGPALSTAPLE
                     VLEVDPKVALIVKTSGSTGNPKSVLLTSQSLIASARASHKFLSASPGQRWSLLLPTTH
                     IAGINVLVRSIELGTVPVGVDNQAEFSAIVPTQLYRAVHSDKQLLEHLQSCKAVLVGG
                     ARLDLELKKKAQDLGITVITTYGMSETSGGVVYDGKALDGVSIQIIDKRIALKGPQIA
                     YGYLEGGFPIQDGWFITSDLGFIQDGDLVVEARADDQIISGGEKISLSAIESYLQDEY
                     SNPQIVAFAKSDAEWGEKLCIATTSNIALDEVRSKLTNKFGSHASPKELVRVSNIPYL
                     SIGKPDRRKLSDDNS"
     gene            2843..3697
                     /gene="menB"
                     /locus_tag="PLW53_04045"
     CDS             2843..3697
                     /gene="menB"
                     /locus_tag="PLW53_04045"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRD36627.1"
                     /translation="MGSRTIPKWEVGQDAREFSDVRYETAEGMAKITINRPQVRNAFR
                     PETLFELERAFTFARDDEKVGVIILTGQGDEAFCSGGDISVRGDDGYIGSDAVGKKGI
                     GRLNVLDLQVQIRRTPKPVIAMVAGWAIGGGHVLHVVCDITIAAENAKFGQTGPMVGS
                     FDGGYGAGLLARHVGQKKAREIWFMCRQYDAKEALEMGLVNTVVPVSELEAETVSWAR
                     EMLRHSPMALRLLKAGLNAADDGLAGVQQLAGDATLLFYLTEEGQEGRDAYKEKREPD
                     FNKFPKRP"
     gene            3701..4675
                     /locus_tag="PLW53_04050"
     CDS             3701..4675
                     /locus_tag="PLW53_04050"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002782.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRD36628.1"
                     /translation="MSLTLEEALASLRVLALPMRTTFRSLNIRETALFKGENGWGEFA
                     PFVEYSDQESLPWLESAIEAADKPLSPALRESIPINATVPASNDEAEIEQILSWYPGV
                     DTVKIKVGTGIQEDLVRIAEVRKHLPKAKIRIDVNGSWSVKEAALNVNAIYEVTGDLL
                     EYVEQPVTSLDELKQLKEGMSVDVKIAGDEVLRKAKDPFVISLDGAIDILMLKVSPLG
                     GIKRAMDLASHHKLPVVISSALESAVGISYGLALAARVPNLDYACGLGTSALFKQDVS
                     DIPIVNGAIKATGYPIDLDRVERYELKGERLEWWRNRISRVWNLRRPA"
     gene            4672..6246
                     /gene="menD"
                     /locus_tag="PLW53_04055"
     CDS             4672..6246
                     /gene="menD"
                     /locus_tag="PLW53_04055"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRD36629.1"
                     /translation="MSNSTALARSIIKQLIELGIKDVVLSPGSRNAPLSIALYEASEK
                     GLINLHVRIDERGAAFFALGIAKASARYVPVVCTSGTAVANYYPALLEAHHSDINLLL
                     LTADRPARLRRTGSNQTTDQSNIFGAFVRASFDTDKELDLSEALSGSGPVHVNLQFDE
                     PLLDKENSDWLSGIKVKTQEKSTGKSIEINIESKKSLVIVGHDRAGFSVAEIERFASD
                     LAAPLIAEDPLVFEGAIAHAPIVLSDEKARSALAAENVFVIGRTTLSRSINAYIQGAK
                     RVIVIDPRTENIDTSRTSDEIYTQIPKLKKPLDIDQSWHELWKKYEALAAIALSVLPE
                     WSEAELATVIAEELEDDTALFISSSRPVRDIESFAVPRSGLITYANRGLAGIDGNIST
                     ALGVATQHSESYAVIGDLAFLHDISALANPTKDNLTIIVVDNNGGGIFSTLGQRGSKG
                     FEKIFGTPHGVDISSVVKSFGISSQQISSAKELHIALQQIHTGLHVIVAKMPDREQNA
                     DSIAAVLKKYSQLVLL"
     gene            complement(6232..7464)
                     /locus_tag="PLW53_04060"
     CDS             complement(6232..7464)
                     /locus_tag="PLW53_04060"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HRD36630.1"
                     /translation="MPISATTELLGEHPELLDIAIADIKDEQITSWVRGGDGVIGFGV
                     YKSHIVKGPNRFEQARSWWRSEISTLDIANNAHSSGSGPILFTSFSFDEAEDSILIIP
                     KVVVGQSNGKSWITWIGDGSQPKLERCEDLARPLNVSWSGSNGDIWRERVALAIGKIK
                     DAKLDKVVLARFLTGKSEERIDVRRLLRLLAKEYPSTWVYSNSGLVGATPELLVRLSK
                     SLITSRVLAGTIRKTGDDERDLALAGSLARSSKDLEEHEYAVQSVADALGPLCRSTNV
                     PETPFVLHLSNVMHLATDVTAVLSDSANPADIFDLASRLHPSAAVCGTPRDLAKKAID
                     EIEGISRGRYAGPVGWIDSKGDGELALALRCGQITSDGKSIRIYAGCGIVAGSDPERE
                     YAESQAKLLPMRSALEKH"
     gene            7516..8211
                     /locus_tag="PLW53_04065"
     CDS             7516..8211
                     /locus_tag="PLW53_04065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD36631.1"
                     /translation="MSRADLGKDPEEVAAMFDGVARRYDLFNDLLSLGATKRWRRSVQ
                     KIISPKPGMKILDIAAGPGSSSEGLHKAGADVTSLDFSKGMLDQGRKVRPYLNFVQGD
                     ALNLAFKENTFDLTTNSFGLRNTQDYKRALREALRVTKPGGRIVIVEFSTPTFKPFRI
                     LYMNYLMKALPKLSRSGSSNPAAYGYLAESIRAWPKQEELARDMEAVGWKNSSWINLT
                     GGVVAVHTAFKGS"
     gene            8252..8614
                     /locus_tag="PLW53_04070"
     CDS             8252..8614
                     /locus_tag="PLW53_04070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRD36632.1"
                     /translation="MSNPYLPIIVIGAIGFGFAIFSVVAGALTGPKRYNRAKLDAYEC
                     GIEPSPQAQGGGRFPVKYFLTAMLFIIFDIEIVFLYPWAVAYDQLALFGLIEMVIFIA
                     TVFVAYTYVWRRGGLEWD"
     gene            8619..9182
                     /locus_tag="PLW53_04075"
     CDS             8619..9182
                     /locus_tag="PLW53_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRD36633.1"
                     /translation="MGLEEKLPSGFLLTTVEKLAGYMRKNSLWPATFGLACCAIEMMA
                     IGSAGKYDISRFGMEVFRASPRQADLMIVAGRVSNKMAPVVRQIYDQMAAPKWVLSMG
                     ACASSGGMFNNYAIVQGVDHIIPVDIYLPGCPPRPEMLMDAILKLHDQIGNEKLGVNR
                     AKIVKEVEASAIAATPIHQIPVFGKQG"
     gene            9186..9824
                     /locus_tag="PLW53_04080"
     CDS             9186..9824
                     /locus_tag="PLW53_04080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRD36634.1"
                     /translation="MSEQKGSFGIQGTGDTSGYGGLVRSSFSPTSAQKPYGSYFDEVT
                     DELERAFPEFNDAIEKVVVDRGELTLHIKRQRLFDVAKTLRDTETLRFEVCLGVSGVH
                     YPEDSQRELHAVYELLSMTHNRRIRLEVSAPDSDPHIPSLVEVWAGTNWHEREAYDMF
                     GIIFDGHPALTRILMPDDWPGHPQRKDYPLGGISVEYVGATIPSPDNRRSYR"
     gene            9821..11149
                     /locus_tag="PLW53_04085"
     CDS             9821..11149
                     /locus_tag="PLW53_04085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRD36635.1"
                     /translation="MSTFASSRETTEGTIYSVTGGDWDQVASEIGSAKEERVVVNMGP
                     QHPSTHGVLRLMLELEGETITELRCGIGYLHTGIEKNTEFRTWTQGVTFVTRMDYLSP
                     LFNESTYCLGVEKLLGITNDVPERASVIRVMMMEFNRISSHMVALGTGALELGAITPM
                     FFAFREREKVLDVFEKVSGLRMNMAYIRPGGVAQDLPDGTVAGIRELIKDLRKNFKDN
                     AKLLIGNSIWIKRTKGVGYLDLAGCITLGVTGPVLRSTGMPWDLRKTQPYCGYENYDF
                     DVITADTCDVYGRFLIRMQELEQSLRIIEQCCDKLDKLEGAPVMVADKKIAWPAQLSL
                     GGDGLGNSLDHIRQIMGTSMEALIHHFKIVTEGFRVPAGQVYTPVESPRGELGAHLVS
                     DGGTRPYRVHFRAPSFSNLQSTSAMCEGSLIADVVAAVASIDPVMGDVDR"
     gene            11149..13077
                     /gene="nuoF"
                     /locus_tag="PLW53_04090"
     CDS             11149..13077
                     /gene="nuoF"
                     /locus_tag="PLW53_04090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRD36636.1"
                     /translation="MPLTDSDNLVMDSIINRYPRSRSAIMPLLHFAQSKDGYVTPESI
                     EVIAKKLNLESAEVSAVATFYTQYKRGPVGEYHVGVCTNTLCAIMGGDEIFESLKEHL
                     GVENNGVTPDGKVSIEHIECNAACDYAPVVMANWEFYDNQSVESAKDLVDSMRQGNPI
                     APTRGPDKLPTWKENSALLAGINDGLANQGVSAGEPTLLGLKIAQSGNKKLTPELTKS
                     YDQKDSFTLDGYRRNGGYKAIEKALNMSPDEVIQAVKDSGLRGRGGAGFPTGMKWGFI
                     PQGDNKEHYFVVNADESEPGTCKDTPLMLANPHVLIEGIIIGSYAIRAKHAFIYIRGE
                     VAHVIRRVQQAIEDAYKAGLLGKNILGKGFDLDLVLHVGAGAYICGEETALLDSLEGF
                     RGQPRLRPPFPAIAGLYARPTVVNNVESVASVPAIINNGAQWFASLGTEKSKGFTLYS
                     LSGHVKNPGQFEAPLGITLRELIDMAGGMRNGNKLKFWTPGGSSTPIFTDEHLDIPLD
                     YEGVAAAGSMLGTKALQIFDETTSVVRAVLRWTEFYKHESCGKCTPCREGTWWLVQIL
                     KDLDAGKGEQADLEKLLDLCDNIAGRSFCALADGAASPIISSIKYFREEYLEHLRIGG
                     SPFSPAASSLLTSDGVKV"
     gene            13074..15428
                     /locus_tag="PLW53_04095"
     CDS             13074..15428
                     /locus_tag="PLW53_04095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HRD36637.1"
                     /translation="MSTQEIATDIAVEMITLTIDGFEVSVPKGTLVIRAAEKLGIQIP
                     RFCDHPLLDPVGACRQCLVEIEVNGRKFPKPQASCTIPVEPGMIVNTQLTSAVADKAQ
                     QGVMELLLINHPLDCPVCDKGGECPLQNQAMSNGREESRFEGTKRTFEKPINISSQVL
                     LDRERCILCARCTRFSEQIAGDPFITLNERGALQQVGIYEKEPFESYFSGNTVQICPV
                     GALTGASYRFRARPFDLISTPSACEHCASGCDMRTDVRRGKTLRRLAGDDAAVNEEWN
                     CDKGRWAFKYVNAKDRITTPLIRDESGQLREASWPEALALAAKGLKENRSAVLVGGRA
                     TLEDAYGYSKFARIALNTNDIDFRSRVTSKEEKNFLASHVVGTQVNYKDLDRADHVLL
                     LGFEAEEESPIVFLRLYKQVKKRELKITAVAPFASRGNKKLKAGLISTPAGSEASVIS
                     KISNLSNKSVILIGERLSESSGAISAAVALAANSGAKLAWIPRRAGERGALEAGALSN
                     LLPGARPVSDNAARVDLQAAWGVSSLPSNPGLDSDEIVKEVEKGNIGALLVGGVDPLD
                     ISPDYQAIMENAFIVSLEIRQSAVTQIADVVLPVAAVVEKSGTFINWESRARFFEAAV
                     PDSLTRSDVRILSMLADEMGTSINLPTVSSARDEFNSLGTWHNRESFTPVNSEATKIA
                     NGKANLCSWRLLLDSGSMQDGENNLAGTAHPSVAVISKNRADSLGVANGDKIRISTPR
                     GEITLPCQIDSIDEDSVWVPRNSINSKVIESLGITSGEVSVVRA"
     gene            15425..16657
                     /gene="nuoH"
                     /locus_tag="PLW53_04100"
     CDS             15425..16657
                     /gene="nuoH"
                     /locus_tag="PLW53_04100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRD36638.1"
                     /translation="MNSASIILDDPTWVIVAKGVLVFALCVVLTLLSVWGERRIVARM
                     QMRMGPNRVGPFGLVQALADGVKLALKEDIIPAAADRAVFVIAPIISVTTCFMAFAVI
                     PLTGEVNFFGEQTIMQLTDLPVGVLYVLATASIGVYGIVLAGWSSGSTYPLLGGLRSS
                     AQVISYEIAMGLSFVGVFIYAGSMSTGDIIAAQSTWWNVVVLFPSFAIYVISMVGETN
                     RAPFDLAEAEGELVGGFHTEYSSLKFALFFLAEYVNIIAVSALATTLFLGGPSAPPGL
                     GFTSAWLGGWFSFIWFFLKVLAFFFFFVWLRGTLPRLRYDQFMKFGWKVLIPVNVAWI
                     LVVATLRLLTQQGASQFVIAGFTGAVVLAVLGAMTIYDRSKIKAEELANQVGPMPEPS
                     FPVPALPTSLALKEKEGN"
     gene            16658..17287
                     /gene="nuoI"
                     /locus_tag="PLW53_04105"
     CDS             16658..17287
                     /gene="nuoI"
                     /locus_tag="PLW53_04105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRD36639.1"
                     /translation="MSEFHLEPKPSGAIQKAADQSVPVTGTEAAGFFTQLKGFWVTFS
                     TMFKKPNTIEYPEVKPEVAPRFHGRHQLNRYEDGLEKCIGCELCAWACPADAIYVEGE
                     NNTEGNQISPGERHAKVYQINYLRCIFCGLCIEACPTRALTMTNEYEIADDTRQKLIW
                     EKEDLLAPLRAGMLMPPHPMYPESDHHNYYRNEIKESHPSQDIKAGGEK"
     gene            17287..18051
                     /locus_tag="PLW53_04110"
     CDS             17287..18051
                     /locus_tag="PLW53_04110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRD36640.1"
                     /translation="MSGESALFWVLAPLAVLASVAMLFMKKAVHSAILLAWVMITLAI
                     FYIALEAPFLGIVQIVVYTGAVMMLFLFILMLVGVDSSDSLVEKIKGIRSVAIFTALA
                     FSLTLITFIARAELGRPSVGLDEANSGGNVEGLAQYLFSDYVWAFEVISALLITAALG
                     AMVLAHSEKSDVARTSQRARSIARFRGKSIATAAGLPGSGVYARNNALDLPALLPDGK
                     PSDLSIAEVLHRRGDVAESKSYQLEGLPKIDDEGNK"
     gene            18051..18350
                     /gene="nuoK"
                     /locus_tag="PLW53_04115"
     CDS             18051..18350
                     /gene="nuoK"
                     /locus_tag="PLW53_04115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRD36641.1"
                     /translation="MNPTNYLYLSAILFSIGAVGVIVRKNAIVVFMCIELMLNAANLA
                     FVTFAKINGNLEGQVMAFFTMVVAACEVVVGLAIIVTIFRSRRSASVDDASLLSR"
     gene            18351..20237
                     /gene="nuoL"
                     /locus_tag="PLW53_04120"
     CDS             18351..20237
                     /gene="nuoL"
                     /locus_tag="PLW53_04120"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRD36642.1"
                     /translation="MTSENLVYLIALPLFSSALLMLLGRKADKWGHVFATLISASTFV
                     VGAMEFFAMIDRPEASRAVTQKLFTWISVGTFNVDAGLLLDQLSIAFVLLITGVGTLI
                     HVYSIAYMSHDRDRRRFFAYLNFFIAAMLLLVLGDSYLNLYVGWEGVGLASYLLIGFW
                     NQKPEYATAAKKAFVANRVGDFGLSVAVMIAFANFGAVSFIGIEEKVGGASEAALTAM
                     GLMLLLAAAGKSAQFPLQAWLGDAMAGPTPVSALIHAATMVTAGVYLITRSNFVFDNA
                     PTAQLAVVIVGTITLLFGAIIGMAKDDIKKALAASTMSQIGYMIMASGFGPAGYAFAI
                     MHLLTHGFFKAGMFLGAGSVMHGMNDEVNMRRYGGLAKLMPITSITFGLGYLAIIGVP
                     PFAGFYSKDKIIETAFNAGGVQGVLFGSAALLGAVITAFYMTRLMLMTFFGNKRWAEG
                     SEPHESPFLMWAPMAVLAVGSVASGYLLYSGKAIVKWLAPVVDKDHHEHAEFLPPIVV
                     TTLAVVAVIIGVSIAFIKYRGELSERAPSEVSIFTRVTRRDLLQDDANEFLFMRPGQK
                     LTQLLVKTDESVIDGAVRAVGSSALGSARGMRKLQTGYVRNYALLILIGALVALFAAL
                     VVTL"
     gene            20234..21745
                     /locus_tag="PLW53_04125"
     CDS             20234..21745
                     /locus_tag="PLW53_04125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRD36643.1"
                     /translation="MNLLTSMGLLPLIGAGLIALLPATNAKLVKQSALLVSVLVAALG
                     ITMTIGFDRGVTGFQYVERYSWIPSLGISYQLGIDGISLILILLSVLLVPIVILAGWN
                     ESEGGRFSVKTFYILILVLETMMIGVFAATDLFLFYVFFEAMLVPVYFLIGGYGSGAR
                     QAAAVKFLLYSLFGGLLMLASIIGIFVISGNQIGRTFDITALSTLEIDATTQNFLFLG
                     FFIAFAIKAPLWPLHTWLPDAAKSATPGTSVLLLGVLDKVGTFGMIRYCVELFPDASR
                     TFTPLIITLAVISIIYGAFLAIGQKDIKSLIAFTSISHFGFITLGIFAMTTQSNSGAT
                     LYMFNHGISTAALFLTAGWMISRRGSSTIADFGGLQRVTPILAWSFFIAGLSSLALPG
                     LSSFVSEFLVLVGTYTRYPVAAVIATFGIILAALYILIPVQKALHGPTTAGNENLPDL
                     NRREIAAIAPVIALIVALGFYPKPALEIINPAAQVTITKAGYSDPQPLVGGDK"
     gene            21745..23262
                     /gene="nuoN"
                     /locus_tag="PLW53_04130"
     CDS             21745..23262
                     /gene="nuoN"
                     /locus_tag="PLW53_04130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRD36644.1"
                     /translation="MGFQSPALEYGALAPMLIIFGAAIVGVIVEAFLSAKVRAPIQLA
                     ISLGAVVLSLSQVWRLRGSATTTAAVNSVVIDGPVLFMQGTILLLSLFAFLLIADIDA
                     FAAQASAVPGSNEEARALESGKIQTEVYPLTLFAIGGMMLFPAANDLITLFVALEVLS
                     LPLYLMAGLSRRRRLLSQEAALKYFLLGAYSSAFFLFGAAFLYGFAGDVSLAGIRSAV
                     SGGPGNDVFLIIGMVSLSVGLLFKVGAAPFHSWTPDVYQGAPTPITAFMAAATKVAAF
                     GAMLRIFYVSLGGAVYSWKPIFTAVAILTMVVGSVIAIAQRDIKRMLAYSSIAHAGFL
                     LTGVIALNQEGLKASLFYLASYGIATIGAFALVTLVRDSSGEVGDLNRWVGLAKRSPA
                     IALIFSLFLLSFAGIPLTAGFIGKFTIFTAAYESGNIAVVVAGVLSSAVAAFFYIRVI
                     VMIFFSDPVEDTVTVTIPSLYTNLTIWIAAIATLALGIFPAPFLDFIATFATFTR"
     gene            23265..24251
                     /locus_tag="PLW53_04135"
     CDS             23265..24251
                     /locus_tag="PLW53_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD36645.1"
                     /translation="MASLGIANLDKDLERELLTGLDQVEALLSSHIQGDYPLLIETSR
                     HLVEAGGKRFRPLITLLASHFGKGQSNQVIAAAVVCELTHVATLYHDDVMDEAKLRRG
                     VSSANSRWSNTVAILTGDYLFSKASDLLADLGPEAVRLQAKTFERLVIGQIQETQGAK
                     NGEDALKHYLQVVSDKTGSLIATSARFGAMLSGADREIVETLTKFGEKIGIAFQLADD
                     VIDISSDASQSGKVPGTDLKEGIPTLVTLQIISSTLDEDQELRQMLQNPIPEEKIAEV
                     LTKLRQHRALKDAKSYLHNLSREAKQLLEPLPAIPARSALEGLCDAVIERTA"
     gene            complement(24210..25109)
                     /gene="rarD"
                     /locus_tag="PLW53_04140"
     CDS             complement(24210..25109)
                     /gene="rarD"
                     /locus_tag="PLW53_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRD36646.1"
                     /translation="MTKFNKGLLFGISAYIIWGLLPLYWKLVEEAGAYEILAHRGIWS
                     LLICISLLALRKQLRSAYVMVRSSRTLSLLFLASGLLTINWGVYIWSVTVNRVVEAAL
                     GYYITPLINVTFGVLLLREKLRPAQWIAVALAAVGVVILTLGYGSLPWIALVLAISWG
                     SYSLIKKSLNLGALETLSLETLFAFLPNLVFLLIIESNGSAEFGSTWTISLLLFGAGA
                     ATVIPLLLFNGSTTRLPLSTVGLLQYITPTIMFFIGIFINNEDISMTKVIGFAFIWLA
                     LAVLSRDLYRSSRPLDDGIAKAL"
     gene            complement(25106..25594)
                     /locus_tag="PLW53_04145"
     CDS             complement(25106..25594)
                     /locus_tag="PLW53_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRD36647.1"
                     /translation="MADSSFDVVSKVDRMELDNALNQASREVATRFDFKNTGVKIEMS
                     GEKILVEAETEERVKAALDVLKEKMIKRAVSLKHLDIAEPAQSGKVFRIFCAIQEGIS
                     QENAKKITKLLKDEGPKSVRAQIQGEELRVSSKSRDDLQSAIALIKGAKLDFAVQFTN
                     YR"
     gene            25748..26635
                     /locus_tag="PLW53_04150"
     CDS             25748..26635
                     /locus_tag="PLW53_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HRD36648.1"
                     /translation="MNSEMFAQVKSGKGFIAALDQSGGSTPKTLALYGVTESAYSNEA
                     EMFDRMHEMRSRLIKSPVFNGERVIGAILFEMTMERTIDGMGSAEFLWNKKKVVPFLK
                     VDNGLADEANSVQLMKPIPELGARIAAANKHGVFGTKMRSVINMANPSGIDEIVKQQF
                     EVGKEIIAGGLVPIIEPEVNIKSAEKAEAEEILKKQLIDQANSLNGDQNIMLKLSLPT
                     KANLYKELVDHPRVVRVVALSGGYSREVANQMLAENTGVIASFSRALAEGLSAQQSDT
                     DFDAMLNATIQSIYEASIK"
     gene            26696..26777
                     /locus_tag="PLW53_04155"
     tRNA            26696..26777
                     /locus_tag="PLW53_04155"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26730..26732,aa:Tyr,seq:gta)
     gene            complement(26787..27500)
                     /locus_tag="PLW53_04160"
     CDS             complement(26787..27500)
                     /locus_tag="PLW53_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRD36649.1"
                     /translation="MRKVNRITAAITALFVLGALWVYPAQGAESDRHITVSGVGTVSV
                     VPDAVRFYFSVTSLSDKSASALSSASKAASGVRAALKSEDIATKDIKSSNLSVYPEYN
                     YTQEKGSVIIGYRATQSFTVVIRKAESAGKVVESVVNAGNESVAINGIAPFISKGSAA
                     MQEARAAAVADAKSKASSYAKLLGTSLGKVISLQENSAPSYSFPMLGVAKAEDASTPV
                     IDLGEEEVSISITVKWALN"
     gene            27519..28478
                     /locus_tag="PLW53_04165"
     CDS             27519..28478
                     /locus_tag="PLW53_04165"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRD36650.1"
                     /translation="MAMIISQCSLSVLRQSAEKLRAGDLVAFPTETVYGIGADAMNKD
                     AVARMYQVKNRPASHPVIVHIAELNDVDYWAQEIPEYAINLMRDFWPGPMTLLLKRRE
                     VAGDFLTGAQEIVGLRIPANSLALGLLNEFKAVGGKGIAAPSANRYGAVSPTTAEAVS
                     QELSEFLSPSDQILDGGPCLVGVESTIIDCSGQNPKILRPGAISAEMIEESTGIVLEE
                     VGETKIRVSGSDKQHYAPKAKVIVDGQSQRGEGLIAMSDVATPEGVIRLAEPKSVEEY
                     ARVLYSALRMSDTQNLQTVRIIPPVGPGLAVAIRDRINRAAAN"
BASE COUNT         7864 a         5996 c         6496 g         8122 t
ORIGIN      
        1 aaactctgca aagcctgcta gctctccatc aaaatagatt tcaaatctat tgagcacttc
       61 acttttcaag atagaaatac tcataagagc accgtatcaa aaaggactat tgttgataag
      121 aaacccgcca tacttcataa ttctgatatg tctactgaga taactgttac agaactctca
      181 agcgatgatt ggcctcgttt aagagatctg cgactagcag cccttgctga tagcccagca
      241 atacttgcag gcaaattaga tgaagaacga aatttcacag aagagcaatg gcgagagact
      301 ttcaagaaac tttcatatgt tgtggcatca attgatggaa aagatgtagc aatgattaat
      361 gttgaaaact tggtaggaga ctttggggca acatgttggc ttggtggtct ctggtctaat
      421 ccagagtatc gaggatctgg cgcggttaga gcgattttta attacatgga tgctgttgct
      481 agtaagcgtg gctggatggt tcaaggcttg ggagtgatgg agagcaatac cagcgccatt
      541 gctgtttttg aaaagtatgg ttttacgaaa cgaggcgacc gcgttgaaag tagaggaaag
      601 ccaggaagct attactttag aatgattaag agcttataac ttcccaagaa aatcaggatt
      661 atcatctggc ggaataattt tccgcttgcg ccttccgcgc ccgtttcctt ttgggcgacc
      721 agcaataatc caggctatag atccaatagc tggaacaaaa ataatgatga ctagccaagc
      781 ccatttaggc aaacttctaa tgttattttc tgaagtgcgc gagcaatcaa tgatgctaaa
      841 tatcatcaaa gccagagtga taattgcagc tattactgcc catttgacca tggatttatt
      901 ttaccgcgca actaacaagg ccgctaaaga aagagctgca gcataaataa tctgaattcg
      961 accagtttta gccaggaggt ccactagttc tatcccgctt gcgccttgct ttactgatct
     1021 aacagattta atcgcaagtg gcgcgcttat taaagctatt aggtagtagg gggagaaaat
     1081 acttaaagca gctgaaatca ctagagcaaa gaagattagc catcgatata aatcgcggct
     1141 ctgggcatcg ccgatgatta ctgccagggt tctctttcct acctgagcat ctttaggaag
     1201 atctcttaag ttatttaagg taagaattgc acatgagaga gcccctagag caaatgaagc
     1261 aagaaggacc tctgatgata attgctcaac atgaacgaag taagttccat ttgtagcaat
     1321 aactccaaag aagataaata ctgagacctc gccaaatccc atatatccat aaggtttaga
     1381 accagcggta tatccccaag cagcagcgat tgagattaaa ccaattggaa ttagcgccca
     1441 ggaagttcta taagagagca aaagcccagc gagggcagct agaacaaagg ataggaaagc
     1501 tgcatttctt actctgattg gttttgcaag ccctgatgcc accaagcgaa ttggtccaac
     1561 tcggtcgtta tccgtacctt taattccatc tgaataatca tttgcataat tgactccaat
     1621 ttgaaggaat aaagcaacga ttaaagctag aaaaaagtga agtagtgaag cgttatagcc
     1681 agcaaatgca gtgccaacta atactggcgc tattgcagca tagagcgttt tcggtcttgc
     1741 cccctgaacc cactgctcag ctgttgtcat cacttaactt tcttctatcg ggctttccta
     1801 ttgatagata aggaatatta gaaactctta ctagctcctt tggtgaggca tgagagccga
     1861 atttattagt taacttactt cttacctcat cgagtgcaat attgctggtt gttgcgatac
     1921 agagcttctc tccccattca gcatctgatt tggcaaatgc cacgatttgg ggatttgaat
     1981 actcatcttg cagatagctc tcgatagcac ttagggaaat tttttcaccg cccgagataa
     2041 tttgatcatc tgctcttgcc tccactacta aatctccatc ttggataaaa cccaagtcac
     2101 ttgttataaa ccaaccatct tgaataggaa agccgccctc caaataacca taggcaatct
     2161 gtgggccttt aagagcaatt cgcttatcga taatttggat tgaaactcca tcaagtgcct
     2221 tgccatcata aacaacgcca cctgatgttt cactcatgcc gtaggtagta attactgtaa
     2281 tccctaaatc ttgagctttc ttttttaatt caagatctaa acgagctcca ccaacaagta
     2341 cagctttaca gctctgcaaa tgttctaata gttgcttatc tgagtggacc gctcgataca
     2401 actgcgttgg aacgattgcg ctaaattcag cctggttatc taccccaacc ggtactgtgc
     2461 caagttctat tgacctaact aaaacattaa ttcctgcaat gtgagttgtt ggtaagagaa
     2521 gagaccatct ctgcccaggg ctagcgctta agaacttatg acttgctctt gcacttgcaa
     2581 taagtgattg agaggtaaga agaactgatt tgggatttcc ggtagagcca gaggttttta
     2641 caattagagc taccttgggg tcaacctcaa gcacttctaa cggcgcggta ctaagggctg
     2701 gtccatcctt tagggccagc aagagctttt caagaaagtg tggcatcgac cacgcaggct
     2761 cgatgatctg cacttctcgc tttaccgcaa gttccataac cgacataggg tatgccatat
     2821 gagcaagcca attaagcgcc agatgggtag tcgaaccatt ccaaagtggg aagttgggca
     2881 agatgcgcgt gaatttagcg atgtccgtta tgaaactgca gagggtatgg cgaagataac
     2941 aatcaatcgt ccccaggtgc gcaatgcatt tcgaccagag acactttttg aattagagcg
     3001 cgcatttacc tttgcaagag atgatgaaaa agttggcgta ataattttga ctggccaagg
     3061 tgatgaagct ttctgctctg gcggcgatat ttcagttcgc ggcgatgatg gatatattgg
     3121 aagtgatgca gttggaaaaa aaggcattgg aagattaaat gttttagatc ttcaagtgca
     3181 gattcgccgc actccaaaac cagtaattgc aatggttgcg ggctgggcaa ttggcggcgg
     3241 acatgttcta catgtggtct gcgatatcac tatcgctgca gagaatgcca agtttggtca
     3301 gaccggacca atggttggat cttttgatgg tggatatggc gcaggtctac ttgcaagaca
     3361 tgttggtcag aaaaaagctc gtgagatttg gtttatgtgt cgccaatatg atgccaaaga
     3421 agctttagag atgggacttg taaatactgt cgttccggtt agcgaactag aggctgagac
     3481 tgtttcttgg gcacgagaga tgttgcgcca ctctcctatg gcacttcgcc tattgaaagc
     3541 gggacttaat gcagctgatg atggacttgc tggagttcaa cagttagccg gagatgccac
     3601 tctgctcttt tacttgactg aggaaggcca agaaggtcgg gatgcatata aagaaaagcg
     3661 cgaacctgac tttaataagt ttcctaaacg tccttaaaaa atgtcactga cgcttgagga
     3721 ggccttagcc tcacttcgag ttcttgcact tcccatgcgc actacatttc gctcacttaa
     3781 tattcgagaa acagcgctat ttaaaggtga aaatggctgg ggagagtttg cgccatttgt
     3841 tgagtattca gatcaagaat cactgccatg gctagaaagc gccattgaag ctgcagataa
     3901 accactttca ccagcacttc gtgaatctat tcctataaat gccacagttc cagcatcaaa
     3961 tgatgaagca gagattgagc agatcctttc ttggtatcca ggagttgata ctgtgaagat
     4021 aaaagttgga acagggatcc aagaggattt agtgcgcatt gcggaggtaa gaaaacacct
     4081 tccaaaagca aagattcgca ttgatgttaa cggatcatgg agcgttaaag aggcagcctt
     4141 aaatgtcaat gctatctatg aagtaacggg agatttactt gagtatgtcg agcagcctgt
     4201 tactagctta gatgagttaa agcaattgaa ggaaggcatg agtgtagatg tgaagattgc
     4261 cggcgatgaa gttcttagaa aagcgaaaga tccatttgtg ataagccttg atggagcaat
     4321 tgatattttg atgttaaaag tttccccact tggtggcata aagcgagcaa tggatttagc
     4381 aagccatcat aagttgccag ttgtaatctc tagcgcttta gagtcagcag ttgggatttc
     4441 ttatggccta gcccttgctg caagagttcc caatctagat tatgcctgcg gccttggaac
     4501 tagtgctctc tttaaacaag atgtgagcga tattccaatt gttaatgggg cgataaaggc
     4561 tacgggctat ccaattgatt tagaccgagt tgagagatat gaattaaagg gtgagcgcct
     4621 ggagtggtgg cgcaatagaa tcagtcgagt atggaatttg aggcgcccag catgagtaat
     4681 tcgactgcgc ttgcgcgaag cattattaag caattaatcg agctagggat taaagatgta
     4741 gtactttctc cgggctctcg caacgcgcct ttatctatcg ccctttatga agcatcagag
     4801 aagggcctaa taaatcttca tgttcgcatc gatgagagag gggcagcatt ttttgctctt
     4861 ggaatcgcta aggccagcgc tcgatatgtt ccagttgtct gcactagtgg aacagcagta
     4921 gctaactact atccagccct tcttgaagcc catcacagcg atatcaattt acttcttcta
     4981 acggcagata gacctgcacg tcttcgaaga actggctcga atcaaacaac agatcagagc
     5041 aatatctttg gcgctttcgt aagggcaagt tttgataccg ataaagagct tgatttatca
     5101 gaggctctaa gtggaagcgg gccagttcat gtgaatctgc aatttgatga accacttctt
     5161 gataaagaaa actctgattg gctctctggg ataaaagtta agactcaaga aaagagcacg
     5221 ggaaaatcta ttgagataaa tattgaatca aagaagtctc tagtcatagt tggtcatgat
     5281 cgagcaggat ttagtgttgc cgaaattgag cgatttgcaa gtgacttagc agcgccactt
     5341 attgctgaag atccattagt ttttgaaggc gcaattgctc atgctccgat tgtcttatcc
     5401 gatgaaaaag caagaagtgc cttagctgca gaaaatgtat ttgtaattgg aagaactact
     5461 ctttctagaa gcatcaatgc ttatattcaa ggggcaaaga gggtaatagt tattgatccg
     5521 cgcaccgaaa acattgacac atctagaact tcagatgaga tttataccca gatcccaaag
     5581 cttaaaaagc ctttagatat tgatcaaagt tggcatgaat tatggaagaa gtatgaagcg
     5641 ctagctgcta ttgctttgtc agttcttcca gagtggtccg aggctgagtt agcaacagtt
     5701 atcgccgagg aactagaaga tgacacagct ttatttatct catcctctag accagtaaga
     5761 gatattgaaa gttttgctgt gccaagatca ggcttaataa cttatgccaa tcgaggtctt
     5821 gcaggaattg atggcaatat ctcaactgct cttggtgttg ccacgcaaca tagcgagagt
     5881 tatgcagtaa ttggagattt agctttcctt cacgatattt ctgcccttgc caatccaaca
     5941 aaagataact tgaccatcat tgttgttgat aataatggtg gcgggatctt ctcaactctt
     6001 gggcagagag gcagtaaagg ctttgaaaag atttttggca caccacacgg ggttgatatt
     6061 tcaagtgtgg ttaagagctt tggaatctca tcgcagcaaa ttagtagcgc aaaggagctt
     6121 catattgcgc tgcaacaaat ccacaccgga cttcatgtaa ttgtggccaa gatgcctgat
     6181 agagagcaaa acgctgattc aattgctgcg gtattaaaga aatatagtca gttagtgctt
     6241 ctctagagca gagcgcattg gcagaagctt tgcctggctc tctgcgtatt ctcgttccgg
     6301 atcagatcca gcaacaattc cacatccagc atagatccga atcgacttac catcacttgt
     6361 aatctgacca caacgcagcg caagtgctaa ctcaccatct cccttgctat ctatccagcc
     6421 aacaggacct gcataacgac cacgtgaaat accctcgatt tcatcaattg ctttctttgc
     6481 tagatctcgc ggagttccac agacggcagc tgatggatgt agcctggaag ctagatcaaa
     6541 aatatctgct gggtttgcag agtcagataa gaccgctgtt acatcggtgg caagatgcat
     6601 gacattagat agatgcagaa caaatggagt ttcaggaaca ttagttgagc ggcataacgg
     6661 acctagcgca tcagcaactg attgaactgc atattcatgc tcttccaaat cctttgatga
     6721 tctagcaaga gagccagcaa gagctaaatc cctttcgtca tcaccagttt ttcgaatcgt
     6781 tccggctaag accctcgaag tgattagaga tttactcaaa cgaaccaata actctggagt
     6841 tgcgccaact aaaccgctat tggaataaac ccaggttgag ggatattcct tggcaagcag
     6901 ccttaatagc cttcgaacat caattctctc ttcactcttt ccggttaaga atcgagctag
     6961 tacgacttta tctagctttg catctttaat ctttccaata gcaagtgcta ctcgctctcg
     7021 ccagatatct ccattacttc cgctccaaga gacatttaat ggcctagcta gatcttcaca
     7081 tctttcaagc tttggctggg aaccatcacc gatccaagta atccaagatt tgccatttga
     7141 ttgacccaca acaactttag gaatgatcaa aatggaatct tctgcttcat caaatgaaaa
     7201 agaggtaaag agaattggtc cactgcctga actatgggca ttattggcaa tatctaatgt
     7261 acttatctca gatcgccacc atgagcgagc ctgctcaaaa cggtttggcc ccttaacaat
     7321 gtgggattta taaactccaa aaccaattac gccatccccg cctctaaccc aagaggttat
     7381 ttgttcatcc ttaatatctg caattgcgat atcaagaagc tcggggtgtt caccaagtag
     7441 ctcggtggtt gcagagatag gcattgggct ccattcaact tgtggagtta gcctagcggt
     7501 agagatgata ggtaagtgag tcgagcagat ttaggtaaag atccagaaga agttgccgcc
     7561 atgtttgacg gggtagctcg tcgctatgac ttatttaacg atttacttag cctgggcgca
     7621 accaaaagat ggcgaagaag tgtgcagaaa attattagcc caaaaccagg gatgaaaatt
     7681 cttgatattg cagcaggccc tggttcatct agtgaaggct tacataaggc gggagcagat
     7741 gtcacctcac ttgatttttc aaaaggaatg ttagatcagg gaagaaaggt tcgcccctac
     7801 ttaaactttg tccaaggcga tgcactcaat cttgccttta aggaaaatac ctttgatctg
     7861 acaacaaatt cttttggttt aagaaatacc caagattata aaagagcttt acgtgaggca
     7921 ctaagagtta ctaaacctgg gggaagaatt gttatcgtag agttctcgac tcccaccttt
     7981 aaaccatttc gaattcttta tatgaattac ttaatgaaag ccttgccaaa attatctaga
     8041 tcaggatcat caaaccctgc tgcatatggc tatcttgcag aatcaatccg cgcctggccc
     8101 aagcaggagg agctagcaag ggatatggag gcggtgggct ggaagaattc atcatggatt
     8161 aacctcacag ggggagttgt ggcagttcac acagccttta agggctccta gatttctctg
     8221 aatttctaag gctcacctaa gatttggcgt tgtgagcaac ccatatctac caatcatcgt
     8281 aatcggagcg attggatttg gattcgccat tttttcagtg gttgccggag ctctaaccgg
     8341 acctaagcga tataacagag caaagctaga tgcttacgaa tgtggaatag agccaagtcc
     8401 ccaagctcaa ggcggcggac gctttccggt caaatacttt ttaactgcaa tgctctttat
     8461 tatctttgat atcgaaattg ttttcctcta tccctgggca gttgcttatg atcaactagc
     8521 tctatttggt cttattgaaa tggttatctt tattgcaact gtatttgtgg cctataccta
     8581 tgtctggcgc cgcggcggat tggaatggga ttaagaaaat gggcttagaa gagaaattac
     8641 cttccggttt tctactcaca actgtagaaa aacttgccgg ctatatgcgc aagaactcgc
     8701 tctggcctgc aacttttggt cttgcttgct gcgctattga gatgatggca atcggctctg
     8761 ccggtaaata cgacatctct cgatttggta tggaagtttt ccgcgcttca ccaagacaag
     8821 cagatttaat gattgtggct ggtcgagtat caaacaagat ggctccggta gttagacaga
     8881 tttatgatca gatggcagct cctaagtggg ttctatcaat gggagcatgc gcttcatctg
     8941 gtggaatgtt taacaattat gcaatcgtcc aaggcgttga tcacataatc ccagttgata
     9001 tttatcttcc aggttgtccg ccaaggccag agatgttgat ggatgcaatt ttgaaacttc
     9061 atgatcaaat tggtaacgaa aagcttggag taaatcgcgc gaaaattgtt aaggaagttg
     9121 aagcatcagc tattgctgct actcctattc atcagatccc agtatttgga aagcagggct
     9181 gattaatgag cgagcagaag ggttcatttg gcatccaggg cactggagat acttctggat
     9241 atggcggcct ggttcgctct agcttctcac caacttcagc acaaaaacca tacggttcat
     9301 actttgatga agtaacagat gaattagagc gcgcatttcc agaatttaac gatgctattg
     9361 aaaaagttgt tgtagatcgc ggggaattaa cactgcatat taaaagacag cgcctatttg
     9421 atgttgctaa aaccctacga gataccgaga ctcttcgctt tgaggtatgt cttggagtat
     9481 caggagttca ttatccagaa gatagccagc gcgaacttca tgcagtctat gaactcttat
     9541 ccatgacaca taatcgtaga attagattag aagtaagcgc tcctgattca gatccacaca
     9601 ttccatcttt ggttgaagtt tgggctggaa ctaattggca tgagcgcgag gcttatgaca
     9661 tgtttgggat tatctttgat ggtcatccag ctctgacaag aattcttatg ccagatgatt
     9721 ggccaggaca tccccagcgc aaggattatc cactcggtgg tatttctgtt gaatatgtag
     9781 gagccacaat cccatctcct gataacagga ggtcatatcg atgagtactt tcgcatcatc
     9841 tcgcgagaca actgaaggaa ccatctattc agttactggc ggcgattggg atcaagtagc
     9901 aagtgagatt ggtagcgcaa aagaagagcg cgtagtagta aacatgggac ctcagcatcc
     9961 atctacacat ggtgtgctgc gcttaatgct tgaacttgaa ggcgaaacaa ttaccgaact
    10021 tagatgtgga attggctatc tacatactgg aattgaaaaa aacactgaat ttcgcacctg
    10081 gacccaaggc gtgactttcg tgacccgaat ggattattta agcccgctat ttaatgaaag
    10141 cacttactgt ctaggggtag aaaagctttt aggcatcact aatgatgtcc ctgagcgagc
    10201 atctgttatt cgagtaatga tgatggaatt taatcgcatc tcatcccaca tggtcgcact
    10261 tggaactgga gcgctagaac ttggcgcaat aactcctatg ttttttgcct tccgcgaaag
    10321 agagaaagtt ctagatgtat ttgaaaaagt ctcagggcta agaatgaaca tggcatatat
    10381 ccgtcctggt ggagttgctc aagatctgcc agatggaacc gttgctggga ttagagaatt
    10441 gattaaggat ttaagaaaga attttaaaga taatgccaag ctattgattg gtaattcaat
    10501 ctggatcaag agaacaaaag gcgttggcta tttagatctt gcaggttgta tcacccttgg
    10561 agttactggc ccagttcttc gctcaaccgg tatgccttgg gatttaagaa agacacaacc
    10621 atattgtgga tatgaaaatt atgactttga tgtcatcaca gctgatacct gcgatgtcta
    10681 tggacgcttt ttaattcgca tgcaggagct tgaacaatcc ttaagaatca ttgagcagtg
    10741 ctgcgacaag ctagataaac ttgaaggagc gccagtgatg gtggccgata agaagattgc
    10801 ttggccagct cagctctcac ttggcggaga tggccttgga aattctctcg atcacattcg
    10861 ccagatcatg ggaacttcta tggaggcgct aatccatcac ttcaagattg tcactgaagg
    10921 ttttagagtt ccagccggtc aggtctacac cccagttgaa tcacctagag gtgaacttgg
    10981 agcccattta gtctccgatg gcggcactcg cccatatcgc gttcatttcc gtgctccttc
    11041 atttagtaat cttcaatcga catctgcaat gtgtgaaggc tcgctaatag ctgatgtcgt
    11101 tgcagctgtt gcttccatcg atcctgtgat gggagatgtg gaccgctaat gccattaact
    11161 gattctgata atttagtaat ggattcgatt attaatcgat atccacgttc gcgttctgcc
    11221 atcatgccac ttctgcattt tgcgcaatct aaagatggat atgtgactcc tgaaagtatt
    11281 gaagtaattg ctaagaagtt aaatctagaa agtgctgaag ttagtgcggt agcaactttt
    11341 tatactcaat ataaacgtgg ccctgtcggt gaatatcatg ttggagtctg cactaacacc
    11401 ttatgtgcaa ttatgggtgg ggatgagata tttgaatcgc ttaaggaaca tcttggtgtt
    11461 gaaaacaatg gagtaacgcc agatggcaaa gtttcaatag agcatattga atgtaatgct
    11521 gcttgtgact acgcacctgt tgttatggca aactgggagt tctacgacaa tcagagcgtt
    11581 gaatcagcta aagatctagt tgactcaatg cgccaaggaa atccaatagc tccaactcgc
    11641 ggcccagata aattaccaac ttggaaagag aactcagcac ttcttgcagg tattaatgat
    11701 ggacttgcaa accaaggggt ttcggcagga gagccaactc ttctagggtt aaagattgcg
    11761 caaagtggca ataagaaatt aactccagaa ttaaccaaga gctatgatca aaaggattca
    11821 tttacccttg atggctatcg tagaaatggc ggctataaag ctattgaaaa ggctctaaat
    11881 atgagcccag atgaagtgat tcaagctgtt aaggattcag gtctacgcgg ccgcggcggc
    11941 gcaggttttc caactggtat gaagtggggc tttatcccgc aaggcgataa caaggaacat
    12001 tactttgttg ttaatgctga tgaatcagag ccaggtactt gtaaagatac cccgctaatg
    12061 cttgctaatc ctcatgtttt aattgaagga ataatcattg gctcttatgc aattagagca
    12121 aaacatgcat ttatctatat tcgcggcgaa gttgcccatg taattcgtcg agttcaacaa
    12181 gccattgaag atgcctataa agcagggctg cttggaaaga acatcttagg taaaggtttt
    12241 gatttagatc tagtacttca tgttggtgct ggcgcatata tttgtggtga agaaactgcg
    12301 cttcttgatt ctcttgaagg tttccgcgga caaccaagac ttcgcccacc atttccagca
    12361 atagcaggtt tatatgcccg cccaactgtg gttaataacg ttgaatcagt tgcatcggtt
    12421 ccagcaatta ttaataacgg agcccaatgg tttgcatcac ttggaacaga gaagagcaaa
    12481 ggctttacgc tctactcact ttcaggacat gtaaaaaatc ctggacagtt tgaagcccca
    12541 cttggcataa cgcttcgcga gctaattgat atggctgggg gaatgcgtaa tggcaataag
    12601 ttgaagttct ggactcctgg tggttcatcc actccgatat ttactgatga acatctagat
    12661 attccacttg attatgaagg cgttgccgca gctggttcaa tgcttggaac caaagcgctg
    12721 caaatatttg atgagacaac tagcgtagtt agagcggtcc ttcgctggac tgagttttat
    12781 aagcatgaat catgcggaaa atgcacccct tgccgtgagg gcacctggtg gttagttcaa
    12841 atccttaaag atcttgatgc aggaaaaggc gagcaagcag atcttgagaa attacttgat
    12901 ctctgcgaca acattgctgg tcgctcattc tgcgcactcg ctgatggcgc agcaagccca
    12961 atcatctcct ccattaaata tttccgtgaa gagtatttag aacatttgag aattggtgga
    13021 tcacctttta gccctgccgc ctcatcactt cttacatcag acggggtgaa ggtatgagta
    13081 ctcaagagat tgcaacagat atagcggtag aaatgattac tctcactatc gatggatttg
    13141 aagtcagcgt gccaaaggga actttagtaa ttagagctgc tgagaaatta ggaattcaga
    13201 ttccacgttt ctgtgatcac ccacttcttg atcctgttgg agcatgccgc caatgtttag
    13261 ttgagattga agttaatgga agaaagtttc caaaaccaca agcctcttgc accattccag
    13321 ttgagccagg aatgattgtt aatactcaac tcacctcagc tgttgccgat aaggcgcaac
    13381 aaggtgttat ggagttatta ctgattaacc acccacttga ttgtccagtt tgcgataagg
    13441 gcggcgagtg tccgctgcag aatcaagcga tgagtaatgg ccgcgaagaa tcaaggtttg
    13501 aaggaacaaa gagaaccttt gaaaagccaa tcaatatttc ctcgcaagtt cttcttgatc
    13561 gtgaacgctg cattctctgc gctcgctgca ctaggttttc tgaacagatt gcaggagatc
    13621 catttattac tctcaatgag cgaggcgccc ttcaacaagt tggcatctat gagaaagaac
    13681 catttgagtc ttacttctca ggaaatacag ttcaaatctg ccctgtggga gccctcactg
    13741 gagcttcata tcgcttccga gcccgtccat ttgatctaat ttcaacccct tctgcctgcg
    13801 aacactgcgc atcaggatgc gatatgcgaa ccgatgttcg tcgcggaaaa actctacgcc
    13861 gcctggcagg agatgatgct gcggttaatg aagagtggaa ctgcgataaa ggtcgttggg
    13921 catttaagta tgtaaatgct aaagatcgca ttactacgcc attaattagg gatgagagtg
    13981 gccaattaag agaggcttca tggccagagg ctttagcact tgcggcaaag ggattaaaag
    14041 aaaatcgctc tgcggtatta gttggcggaa gagcaactct tgaggatgcc tatggatatt
    14101 ccaagtttgc tcgcatcgcc cttaatacaa acgacattga ttttcgctcg cgagtaacat
    14161 caaaggaaga gaagaatttc ttagcctcac atgttgtggg aactcaagtt aattacaaag
    14221 accttgatag agcagatcat gttcttcttc ttggttttga agctgaagaa gaatcaccaa
    14281 ttgtcttcct tcgcctctat aagcaagtga aaaaacgtga attgaaaatt acagctgttg
    14341 ctccttttgc atctcgcgga aataagaagt tgaaagcagg actaatctca accccagctg
    14401 gcagtgaagc ttcagtaatt tcaaaaatct caaatctttc taataaatca gtaatcctta
    14461 ttggagagcg cctctctgag agctctggcg caatttctgc tgcagtggct cttgctgcaa
    14521 atagcggtgc aaagctggct tggattccaa gaagagccgg cgagcgagga gcacttgaag
    14581 ctggagcgct atctaatctt cttccaggag caagaccggt aagcgataac gctgcaaggg
    14641 ttgatcttca agctgcctgg ggtgtttcaa gtcttccaag taaccctggt cttgatagcg
    14701 atgaaatagt aaaagaggta gaaaaaggca atattggcgc ccttttggtt ggcggcgttg
    14761 atccccttga tatctcacct gattaccaag caataatgga aaacgctttt atcgtttcac
    14821 ttgaaattcg ccagagcgca gtaacccaga ttgcagatgt tgtccttcca gttgcggcag
    14881 ttgtcgaaaa gagtggaacg tttattaatt gggaatccag agcaagattc tttgaagcag
    14941 ctgtgccaga ttctctaact agaagcgatg tgagaatcct ttccatgctt gcagatgaga
    15001 tgggaactag cattaatctt ccaaccgtta gctcggcaag agatgaattt aactcacttg
    15061 gtacttggca taaccgtgag agctttactc cggtaaatag tgaagcaaca aaaattgcaa
    15121 acggaaaagc aaatctatgc tcctggagat tgcttcttga ttctggatct atgcaagatg
    15181 gagaaaataa tttagcggga acagcccatc catcagttgc agttatttct aagaaccgcg
    15241 cagattcttt gggtgttgca aatggagata aaattaggat ctcaacgcca agaggcgaga
    15301 taacccttcc ttgccagatt gattcaattg acgaggattc tgtctgggtg cctcgcaata
    15361 gcattaatag caaagtaatt gaaagccttg gtataacttc tggtgaagta tcggtggtga
    15421 gagcatgaat agtgcatcga taattcttga tgatccaacc tgggtaatag ttgccaaagg
    15481 cgttctcgta tttgctctct gtgtcgtact aacacttctc tcggtgtggg gcgagagacg
    15541 tatcgttgct cgaatgcaga tgcgtatggg accaaaccga gttggtccat ttggcttagt
    15601 tcaagcttta gcagatggcg tgaaacttgc tttgaaagag gacatcattc cggcagcagc
    15661 agatcgcgcg gtatttgtta tagccccaat tatcagcgtc actacttgct ttatggcctt
    15721 tgcggttatt cccctgactg gcgaagtaaa tttctttggc gagcagacaa tcatgcaatt
    15781 aactgatctg ccagttggag tcttatatgt tctagcaaca gcatcaattg gggtttatgg
    15841 aattgtttta gcgggctggt cttcaggctc gacatatcca ctacttggtg ggctgcgctc
    15901 tagcgctcaa gttatctcct atgaaattgc tatgggactt tcatttgttg gtgtatttat
    15961 ctatgccgga tccatgtcaa ccggagacat aattgctgct caatcaacat ggtggaatgt
    16021 tgttgttctt ttcccttcat ttgcaattta tgtcatttct atggtgggtg agacaaatcg
    16081 cgcgccattt gatttagccg aagcagaagg cgaattagtt ggcggcttcc atactgagta
    16141 ctcatcactt aagtttgcgc tgttcttctt ggctgaatat gtaaacatca ttgcagtctc
    16201 tgctctcgca actaccttgt ttttaggagg cccaagtgct cctccaggac ttggcttcac
    16261 atcagcttgg cttggtggtt ggtttagctt catctggttc ttcctaaaag ttcttgcatt
    16321 cttctttttc tttgtctggc tacgaggaac tctgccaagg ttgcgttatg accaattcat
    16381 gaaatttggt tggaaagtat taattccagt aaatgttgct tggattttag ttgtcgccac
    16441 acttcgcctt ctaactcagc aaggcgcttc acaatttgtt attgcaggat ttaccggagc
    16501 tgttgtttta gcagttcttg gggcaatgac tatttatgat cgttcaaaaa tcaaggctga
    16561 agagttagca aatcaagttg gtccaatgcc tgagccatca tttccagtcc cagcccttcc
    16621 gacatctcta gctttaaaag aaaaggaggg taattaaatg agtgaatttc acttagaacc
    16681 caagccttca ggggccattc aaaaggctgc cgatcaatca gttcccgtta ctggaactga
    16741 agcagcaggc tttttcaccc agttaaaagg cttctgggtc accttcagca cgatgtttaa
    16801 gaaacccaac accattgaat accctgaagt taaaccggaa gttgcgccac gctttcatgg
    16861 tcgccatcaa ctaaatcgct atgaagatgg tcttgaaaag tgcattggct gtgagctctg
    16921 cgcttgggct tgtcctgcag atgcaatcta tgttgaagga gaaaataaca ccgaagggaa
    16981 tcagatctcc cctggtgagc gacatgccaa ggtttatcag attaattatc tgcgttgtat
    17041 tttctgtggt ctatgtatag aggcttgtcc aacaagagcg ctgacaatga caaatgaata
    17101 cgaaattgct gatgacacac gtcagaaatt aatctgggaa aaagaagatt tacttgctcc
    17161 tcttcgcgct ggaatgttga tgccaccaca tccgatgtat ccagagtctg atcatcacaa
    17221 ttattatcga aatgaaatta aagagtcgca cccatcgcaa gatataaagg caggtggaga
    17281 gaagtaatga gcggtgaatc agctttattc tgggtactag ctcctcttgc cgttttggca
    17341 tcggttgcta tgttatttat gaaaaaagct gttcactcgg caattctgct tgcttgggtg
    17401 atgattacct tagcgatttt ctatattgct ctcgaagcac catttcttgg aattgttcaa
    17461 attgtggtct ataccggagc agtaatgatg ctcttccttt ttatcctgat gttagttgga
    17521 gtggattcat cagattcatt agttgaaaag atcaaaggga ttcgctctgt tgcaatcttt
    17581 acagctctag ccttttcgct aacactcatt accttcatcg ctcgcgcaga gcttggaagg
    17641 ccgagcgttg gcttagatga agccaatagc ggtggaaatg tcgagggctt ggctcaatac
    17701 ctatttagcg attatgtctg ggcttttgag gtcatttcag cgcttctcat caccgcagct
    17761 cttggcgcca tggttcttgc ccactctgaa aagagtgatg tcgcaagaac ttctcagcga
    17821 gcaagatcta tagcgcgctt tcgtggaaaa tcgattgcaa cagcagcagg tcttccaggc
    17881 tctggtgttt atgccagaaa taacgcacta gatcttccag ccttattgcc ggatggaaaa
    17941 ccatctgatc tttcaatcgc agaagtgctt catcgcagag gtgatgtagc tgaatctaag
    18001 agctatcaat tagagggatt gccaaagatt gatgatgagg gcaacaagta atgaatccaa
    18061 caaattatct ctatctctca gcaatcctat tttcaatagg agctgttgga gtaattgttc
    18121 gaaagaatgc aattgttgtt ttcatgtgca ttgaacttat gctcaatgct gccaatctag
    18181 cttttgttac tttcgctaaa attaatggaa atctagaagg tcaagttatg gcctttttca
    18241 ccatggttgt cgcagcatgt gaagttgtag ttggtcttgc aattattgtc actatcttcc
    18301 gctctcgtcg atctgcatca gttgatgatg caagtttgtt gagtcgataa atgacttcag
    18361 agaacttagt ttatttaatc gctctgccgc tttttagctc agcgcttttg atgcttcttg
    18421 gtcgaaaagc tgataaatgg gggcatgtat ttgcaacatt gatttctgca agcacttttg
    18481 tagttggagc gatggaattc tttgcaatga tagatcgccc agaggcatca cgggctgtga
    18541 cccagaagtt atttacttgg atttcagttg gtacatttaa tgttgatgct gggctactcc
    18601 ttgatcaact atcaatagcc ttcgttctac tcattactgg agttggaact ctcattcatg
    18661 tttactccat tgcctatatg tcccatgaca gggatagaag gcgttttttc gcgtatttga
    18721 acttctttat cgcagcaatg ctcctactag ttcttgggga ctcttacctg aacctctatg
    18781 tgggctggga aggagttggc cttgcctcct accttcttat tggcttctgg aatcagaaac
    18841 ctgaatatgc aactgctgcc aagaaagcat ttgtggccaa ccgtgttggt gactttggat
    18901 tatctgttgc agtaatgatt gcttttgcaa actttggcgc tgtttcattt attggaattg
    18961 aagagaaagt tggcggagca agtgaagccg ctcttactgc aatgggtctg atgttgctac
    19021 tggcagcagc gggcaagtca gcacaattcc cacttcaagc ctggcttgga gatgcgatgg
    19081 caggtccaac ccctgtttca gctttaatcc atgccgcaac aatggtgacg gctggagttt
    19141 atttaattac tagatctaac tttgtatttg ataatgcacc aactgcgcaa ctagcagtag
    19201 taatagttgg aactatcacc ttactctttg gcgcaattat tggtatggca aaagatgata
    19261 ttaagaaagc acttgctgcc tcaaccatgt cacaaattgg ctacatgatt atggcctcag
    19321 gctttggtcc tgctggatat gcatttgcaa tcatgcatct tctaactcat ggtttcttta
    19381 aagctggaat gttcttaggt gcaggctcag tcatgcatgg tatgaatgat gaagtaaata
    19441 tgcgacgcta tggtggactc gctaaattaa tgccaattac atcaattact tttggtcttg
    19501 gatacctagc aataattggc gttccgccat ttgcagggtt ttattcaaaa gataaaataa
    19561 ttgaaactgc atttaatgca ggaggcgtac aaggcgttct cttcggctct gcagctctat
    19621 taggggcggt gatcacagcc ttttatatga ctcgtctaat gctcatgact ttcttcggta
    19681 ataagcgctg ggcagaggga agcgagcctc acgaatcacc atttttgatg tgggctccta
    19741 tggcagtttt agctgttggt tcagtcgcct ctggctacct tctctatagc ggcaaggcca
    19801 ttgttaagtg gcttgcgcca gttgttgata aggatcatca cgaacatgca gagtttttgc
    19861 ccccaatagt tgtcacaact cttgcagtag ttgccgtgat aatcggagtt tctattgcct
    19921 ttattaagta tcgcggagag ttatcagaga gagcgccaag tgaggtaagc atctttactc
    19981 gcgtaactcg acgagatctt ctccaagatg atgccaatga gttcctattt atgcgccctg
    20041 gtcagaagtt aactcaacta ttagttaaga ccgacgaaag tgttattgat ggagcagttc
    20101 gggcagttgg ctccagcgcg ctaggaagtg ctagagggat gcgcaaactg caaacaggat
    20161 atgttcgtaa ttacgctcta ctaattctta taggcgcact cgttgctcta tttgccgctt
    20221 tggtggtgac gctatgaacc ttttaacaag tatgggatta ctacctctaa ttggagctgg
    20281 tttaatcgcc ctgcttcctg caactaatgc aaagctagta aaacaaagtg ctctgcttgt
    20341 atctgttttg gttgcagctc ttggaatcac catgacaatt ggatttgata gaggcgtaac
    20401 aggctttcaa tatgttgagc gatattcatg gattccatcc cttggaatta gctatcaact
    20461 tggaatcgat ggaatatcac taattctaat tttgctatca gtcttattag taccaattgt
    20521 gatcctagcg ggatggaatg aatcagaagg tggtcgattt agtgttaaga ctttctatat
    20581 tctgatcctc gttcttgaaa caatgatgat tggtgtcttt gcagcaacag acttattcct
    20641 cttttatgtt ttctttgaag cgatgctcgt cccggtctac ttcctcattg gtggttatgg
    20701 cagcggggca agacaagcag cagcggtaaa gttcttgctt tatagcctct ttggcggtct
    20761 gttaatgctt gcttcaatta ttggcatatt tgtgatttct ggaaatcaaa taggtagaac
    20821 ttttgatatc actgcgcttt caactcttga gatagatgca actactcaga acttcttatt
    20881 cctaggattc tttattgcct ttgccattaa agcaccgctc tggccacttc atacctggct
    20941 tccagatgca gcaaagtcag caactcctgg aacttcagta ctcctacttg gcgtgctgga
    21001 taaagtggga acgtttggaa tgattcgcta ctgcgttgag ctattcccag atgcctctag
    21061 gacttttacg cctttaataa ttactctagc tgtgatttca ataatctatg gagcatttct
    21121 tgcaattggc cagaaggata ttaaatcgct cattgccttc acatctattt ctcactttgg
    21181 ttttataact ttaggtatct ttgcaatgac gactcaatca aattctggag caacgctcta
    21241 tatgtttaat catggaatct caaccgccgc tctcttctta actgctggtt ggatgatctc
    21301 tcgtcgcggc tcttcgacaa ttgctgactt cggaggcttg cagagagtta ctcctattct
    21361 tgcctggtct ttctttattg caggattgtc gtctctagcg ctaccgggcc tatcaagctt
    21421 tgtcagtgaa ttcttagttc tggttggtac ttatactaga tatccagtag ctgcagtgat
    21481 tgcaaccttt ggaattattc tggcagccct atacatttta attcctgtgc agaaagctct
    21541 tcatggccca acaactgctg gaaatgaaaa tctgccagat cttaatagac gcgagattgc
    21601 tgcaattgcg ccagttattg cgctaatagt tgctctaggt ttttacccta agccagccct
    21661 tgaaattatc aatcctgctg cccaagtaac aattactaag gcgggctata gcgatccgca
    21721 gccccttgta ggaggcgata aataatgggc tttcaatcac cagctctgga atatggcgct
    21781 ttagcgccaa tgctaattat ctttggcgca gcaattgttg gagtcattgt tgaagcattc
    21841 ctatcggcaa aagttcgcgc accgattcaa ctggcaatat cactaggcgc tgtagtccta
    21901 agcctttctc aagtttggcg cctaagaggt agcgctacga caacggcagc tgtcaattcc
    21961 gtagtcattg atggtccagt cttgtttatg caaggaacaa ttttacttct ttcgcttttt
    22021 gcattccttc tgattgcaga tattgatgct tttgctgcac aggcctctgc tgtgccaggt
    22081 tctaatgaag aagcaagggc tcttgaatct ggaaagattc aaactgaggt ctatccccta
    22141 accctctttg caatcggcgg catgatgcta tttccagcgg ccaatgattt gatcactttg
    22201 tttgttgctc tagaagttct ctcgctacca ctttatttaa tggcaggact ttccagaaga
    22261 cgcagactct tgtctcagga ggcagcactt aaatacttct tactaggggc gtattcatct
    22321 gccttcttcc tatttggcgc agcatttctc tatggatttg ctggtgatgt ttcacttgca
    22381 ggaattagga gcgctgtctc aggtggccca ggaaatgatg tcttcctaat tattggcatg
    22441 gtctcactta gcgtaggtct gctatttaaa gttggcgcgg ctccatttca ctcctggacc
    22501 cctgatgttt atcaaggagc gcctactcca attactgcct ttatggcagc agccacaaag
    22561 gttgcagcat ttggcgcgat gctccgaatt ttctacgtct cacttggcgg ggcggtctat
    22621 agctggaagc caatctttac tgcagttgca atcctgacca tggttgttgg ctcagttatc
    22681 gctattgcgc agagggatat caagcgaatg ttggcctatt cctcaatagc ccacgctggt
    22741 tttctgctca ccggagttat cgctcttaat caagagggcc taaaggcatc actcttctat
    22801 ctagctagct atggaattgc caccatcggg gcttttgctc tggtgactct agtcagagat
    22861 tcatctggcg aagttggaga tctaaatcgt tgggtgggac tagctaaacg ctctccagcc
    22921 atagctttaa tcttctcgct atttcttctc tcctttgctg gaattccact taccgctggc
    22981 tttattggca aattcacaat ctttactgcc gcctatgaga gtggaaacat tgcagtagta
    23041 gttgcaggtg tcttatcgag cgctgtcgct gccttcttct acataagagt cattgtcatg
    23101 attttcttta gtgatccagt ggaagatact gtgactgtga caattccatc gctttatacc
    23161 aacttaacaa tctggattgc agctattgca actctagctc tcggaatatt tcctgcgccg
    23221 tttcttgatt tcattgcaac cttcgcaacc ttcacacgct aattatggct agcctaggaa
    23281 ttgccaatct cgataaagat cttgaaagag agcttttgac tggccttgat caagtcgaag
    23341 cgctgttaag tagccatatt caaggtgatt acccgttact aatagaaacc tctcgccatt
    23401 tagttgaagc aggagggaaa cgctttcgcc ctctaattac tcttcttgcc tctcattttg
    23461 gcaagggtca gagcaatcaa gttattgcag cagcagttgt ctgtgagctc acccatgtag
    23521 caacgcttta tcacgacgat gtaatggatg aagctaagtt aagacgtgga gtaagtagcg
    23581 ccaatagtcg ttggagcaac actgttgcaa tccttaccgg agattatctc ttctcaaaag
    23641 cctcagattt actggcagat cttgggccag aagcggtccg attgcaggca aagacctttg
    23701 aaaggcttgt tattggccag attcaagaga ctcagggagc aaagaatggt gaagatgcac
    23761 ttaaacacta tcttcaagtt gtttcagata agaccggatc tcttattgca accagcgctc
    23821 gatttggagc gatgctctca ggagctgata gagagatagt tgaaacgcta actaaatttg
    23881 gagaaaaaat tggcattgct tttcaattag cggatgatgt aattgatatc agcagtgatg
    23941 catctcaatc aggcaaagtt ccagggacag atctaaaaga gggaatacca actcttgtaa
    24001 ctctgcagat aattagttct acgttagatg aagatcaaga actaaggcag atgctacaaa
    24061 acccaatacc tgaagaaaaa atcgcagaag tgcttaccaa gcttcgccag cacagggcgc
    24121 ttaaggatgc aaagagctat cttcacaact tatcgcgaga agctaaacaa cttcttgaac
    24181 ctcttccagc aatacctgct cgcagcgctc tagagggcct ttgcgatgcc gtcatcgagc
    24241 ggacggcttg atcgataaag gtctctactt agtactgcaa gagccagcca gataaaagca
    24301 aatccaatca ctttagtcat cgagatatcc tcattattaa taaagatacc aataaaaaac
    24361 atgatggttg gagttatata ttgcaagagt ccaacagtgc ttagtggcaa tctggtagtt
    24421 gatccattaa ataacagcag gggaataaca gttgctgcgc ctgctccaaa aagcagtaag
    24481 gatatggtcc aagtggaacc aaattcagcg ctgccattac tctcgataat gagtagaaaa
    24541 actaagttag gtagaaaagc aaagagtgtt tctaaagata aagtctcaag agctccaaga
    24601 tttaaggact tcttaatcaa gctataactg ccccaagaga tcgccaaaac cagcgcaatc
    24661 caaggaagtg agccatatcc aagggtaaga attactactc caacggctgc cagagccaca
    24721 gcgatccatt gagctggcct taatttctcg cgaagcagta gaactccgaa ggtgacgtta
    24781 attagtggag ttatgtaata gccaagtgct gcttcaacta ctcgattcac agttactgac
    24841 cagatgtaaa cgccccaatt aatcgtcaat aagcctgagg ctaaaaacaa aagagaaagg
    24901 gtgcgcgagc tgcgaaccat cacatatgcg ctcctcaatt gttttcttaa agcaagcagt
    24961 gaaatacaga ttagaagtga ccagatccca cggtgggcca atatttcata tgcgccagct
    25021 tcttcaacta gcttccaata aagtggtagt agtccccaga tgatgtaggc agagatgcca
    25081 aaaagtagac ctttattaaa tttggtcatc gatagttagt gaactgaaca gcaaagtcca
    25141 gtttagctcc cttaattagc gcaatagcgc tctgcaaatc atctctgctt ttacttgaaa
    25201 ctctaagttc ttctccttga atctgtgctc ttacagattt aggtccctca tcttttaata
    25261 gctttgtgat cttcttagca ttttcttgag agattccctc ttgtatcgcg cagaagattc
    25321 gaaatacttt gccgctctga gctggttctg caatatctag atgctttagc gatactgccc
    25381 ttttaatcat cttctctttt aagacatcaa gggccgcttt cacccgctct tctgtttctg
    25441 cctcaactaa gatcttttcg ccagacatct ctatctttac tccggtgttt ttaaaatcaa
    25501 agcgggtggc aacctcacga gaagcctggt ttagagcgtt atctagctcc atccgatcaa
    25561 ctttggaaac gacatcaaaa ctgctatcag ccatggtcat accgtatcgc gtgaggattt
    25621 tgagcgagaa atcgctggtt atttgaagcc ttgctgacct attagaatta gagccttctc
    25681 ggattcagcg ttgtatgcga ggcaatcgga gatttctagc ccaataaaca gctcttaagg
    25741 agtatcgatg aatagcgaaa tgtttgccca agttaaatct ggcaaaggct ttattgcagc
    25801 ccttgatcaa tcaggcggca gcacgccaaa gaccctagcg ctctatggcg ttactgaatc
    25861 tgcatactca aatgaagctg agatgtttga tcgcatgcat gagatgcgct cacgcctaat
    25921 taagtcacca gtatttaatg gcgagcgtgt tatcggcgca attctttttg aaatgactat
    25981 ggagcgcacc attgatggca tgggctctgc tgaattctta tggaataaga agaaagtcgt
    26041 tcctttcctt aaagttgata acggactagc agatgaggca aatagcgttc agttaatgaa
    26101 gccaattcca gaacttggcg caagaattgc cgctgcaaat aagcatggag tctttggcac
    26161 caagatgcgc tcagtaatta atatggcaaa cccatcagga attgatgaaa ttgttaagca
    26221 gcagtttgaa gttggcaaag aaattatcgc aggtggtctt gttccaatca ttgagcctga
    26281 ggtaaatatc aagagcgcag agaaggcaga agctgaagaa attctaaaga agcaattaat
    26341 cgatcaagca aatagcctaa atggcgatca aaatatcatg cttaagctat ctcttccaac
    26401 aaaagccaat ctctataaag agctagtaga tcaccctcgc gttgtaagag tcgttgctct
    26461 ctctggtgga tattcaagag aggttgctaa tcaaatgctt gctgaaaata ctggcgttat
    26521 tgcctctttc tcaagggccc ttgctgaggg gctaagcgct caacaaagcg atactgattt
    26581 tgatgcgatg ctgaatgcaa caattcagag catttatgag gcctcaatca agtaataatt
    26641 agtaatttgt gagcgcgagg gtgagttgct agccttgcgc aatcaagtgc taaatggtgg
    26701 gttgcccgag tggccaatgg gagcggactg taaatccgcc ggcttagcct tcagtggttc
    26761 aaatccacta cccaccacaa gtgctcttag ttcagagccc atttcacggt gatagagata
    26821 gagacttcct cttcaccaag atcaattaca ggagttgagg catcctctgc cttagcaact
    26881 ccaagcattg ggaatgaata tgaaggagca gagttttctt gtagtgaaat gactttgcct
    26941 aatgaggttc caagaagctt ggcataggaa gaggctttag attttgcatc agcaactgct
    27001 gcagccctag cttcctgcat tgctgcagat cctttagaga taaatggcgc gatgccatta
    27061 atagcaacac tttcattacc tgcgttaacc actgattcaa ctacttttcc agcgctctca
    27121 gcttttcgaa ttactactgt aaatgactgt gtagctcgat atccaataat tacagagccc
    27181 ttttcctggg tgtagttgta ttctggatag accgaaaggt ttgaactctt aatatcttta
    27241 gtagcaatat cctctgactt tagcgctgcc ctaacaccag aagctgcttt tgatgcgcta
    27301 gatagcgcac ttgcactctt atcgctaagt gaggtgacgc taaagtaaaa tctcactgca
    27361 tcgggaacaa cgctcactgt tccgactccc gatactgtta tatgacgatc tgattcagct
    27421 ccttgagctg gatagaccca gagcgctcct aggacaaata aagcggtaat ggctgcggta
    27481 attcgattca cttttctcat aagcctaaga tacccgctat ggcaatgatt atttctcaat
    27541 gctcactgag cgtattacgg caaagtgctg agaaattaag ggcaggagac ttagtcgcct
    27601 ttccaacaga aactgtctat ggcattggag ctgatgcgat gaataaagat gcagttgcca
    27661 ggatgtatca ggtgaaaaat aggcccgcta gccacccggt aattgttcat attgcagagc
    27721 taaatgatgt tgattactgg gcacaagaaa ttcctgagta tgcgatcaat ctaatgcgag
    27781 atttctggcc aggcccaatg actcttctcc ttaagagacg agaggttgct ggcgactttc
    27841 taactggagc tcaagagatt gtgggtttaa ggattccggc aaatagcttg gcacttggac
    27901 tactcaatga atttaaagca gttggtggaa aggggattgc tgctcctagc gccaatcgtt
    27961 atggcgccgt ttcaccaaca actgctgagg ctgttagtca ggagctaagt gagtttctat
    28021 cgcctagcga tcaaatactt gatggcgggc cttgtttagt tggagttgaa tcaacaatta
    28081 ttgattgcag cggtcaaaat ccaaaaattc ttcgtccagg agcaattagc gcggagatga
    28141 ttgaagagag cacggggata gttcttgaag aagttggcga aacaaagatt cgagtctcag
    28201 gttctgataa gcagcactat gcaccaaaag caaaagtgat tgttgatggt caaagtcaga
    28261 gaggtgaggg gttaattgca atgagtgatg tcgctacccc agaaggtgtt attcgcttgg
    28321 cagaacccaa aagcgttgaa gagtatgcgc gggtgcttta ttcagctctt cgcatgtcag
    28381 atactcagaa tttgcagact gtgagaataa ttccaccagt aggcccaggc cttgcggttg
    28441 caatccgtga tcgcattaat agagcagcag cgaattag
//
LOCUS       DAOROD010000015        24834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_63_length_24834_cov_33.573873, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000015 DAOROD010000000
VERSION     DAOROD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 24834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24834
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_24834_cov_33.573873"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /gene="fusA"
                     /locus_tag="PLW53_04170"
     CDS             complement(<1..1951)
                     /gene="fusA"
                     /locus_tag="PLW53_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRD36651.1"
                     /translation="MSTATAIDLAKVRNIGIMAHIDAGKTTTTERILFYTGINYKIGE
                     VHEGAATMDWMEQEQERGITITSAATTCEWRGHTINIIDTPGHVDFTVEVERSLRVLD
                     GAVTVFDGVAGVEPQSETVWRQADKYSVPRICFVNKLDRTGANFDRCVEMIGSRLGAT
                     ALVLQVPIGVEGDFIGVVDLIAMKALTWRGETQKGEDYVIEEIPADLKEKCDAARFAL
                     IETLSDADDAIMEKYLEGAAISEDEIIAGIRRATLAYKLTPVLTGSAFKNKGVQPMLD
                     AVTRYLPSPLDVEAIKGTKMGDKSVEIDRKPDPKEPYSSLAFKIMSDPHLGKLTFVRI
                     YSGVLEAGSGVLNSTKDRKERIGKIYRMHANKREEIPQASAGDIIAVMGLKDTTTGDT
                     LCDIDKPVILESMDFPAPVIHVAIEPKTKGDQEKLGVAIQRLAEEDPTFHVRTDEETA
                     QTIIGGMGELHLEILVDRMKREFKVEANVGKPQVAYRETLRKPVARYDYTHKKQTGGS
                     GQFAKIQIALEPLPVGEVEGGYEFVNKVTGGRIPKEYIPSVDDGCQEALQNGPLAGYP
                     LTDVRVTLLDGAYHDVDSSELAFKIAGIAAFKEAAKLAGPVLLEPMMSVEVTTPEDFM
                     GDVIGDLNSRRGQIQAMDERAGARIV"
     gene            complement(1957..2427)
                     /gene="rpsG"
                     /locus_tag="PLW53_04175"
     CDS             complement(1957..2427)
                     /gene="rpsG"
                     /locus_tag="PLW53_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRD36652.1"
                     /translation="MPRKGPAAKSPVIADPVYNSPVVTALINKCLVHGKRSTSEAIVY
                     KALENCRAKTEVDPVITLKKALDNVKPAVEVRSRRVGGATYQVPVEVKAARSMTLGLR
                     WLVDNARLRREKSMAERLANEMIDASNGLGAAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(2427..2801)
                     /gene="rpsL"
                     /locus_tag="PLW53_04180"
     CDS             complement(2427..2801)
                     /gene="rpsL"
                     /locus_tag="PLW53_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRD36653.1"
                     /translation="MPTIQQLVRKGRVDKSDKTTTPGLKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGMEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKVVRGSL
                     DTQGVKDRKQSRSRYGAKKEKK"
     gene            2974..3306
                     /locus_tag="PLW53_04185"
     CDS             2974..3306
                     /locus_tag="PLW53_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD36654.1"
                     /translation="MAGKVKAKKAGKKSTIKNPRRGRATTSRISSKNQITIPVEVLRE
                     VNLKPGDKVEFMIDKQERIILAPSDQASWKKNLMQLAGSMPELGSNFDYKKERQEWDL
                     KATQTPLG"
     gene            3270..3650
                     /locus_tag="PLW53_04190"
     CDS             3270..3650
                     /locus_tag="PLW53_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRD36655.1"
                     /translation="MGLKSHADTSWLIALLNPLDISHKLAIRQLEELNAPASISTFVL
                     AELMVSFEKSDIGSISVTLREIEKSFSPIVELSKEIAISAAQIRSSSKITLGDAIVIA
                     SALDQNSELLTFDKNMKAVYERIK"
     gene            3634..4272
                     /locus_tag="PLW53_04195"
     CDS             3634..4272
                     /locus_tag="PLW53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRD36656.1"
                     /translation="MNVLNRPKLRGIIHLVMSPLALVAGLVLITITTELRGRVTLTIF
                     TLTAVSLFTCSAIYHRVPWGPSAKAIWRRIDHANIPFLIAGTYTPFAIYLLDKSQAVI
                     LLSLVWGGALLSGVFRVFWLNAPRWLYVPIYLALGWAALIYLPDFYRNGGLAVLLLIA
                     FGGILYSLGAIIYAVKWPNFSINWFGFHELFHAMTAAAFISHFIAAILVIVG"
     gene            4275..4976
                     /gene="deoD"
                     /locus_tag="PLW53_04200"
     CDS             4275..4976
                     /gene="deoD"
                     /locus_tag="PLW53_04200"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRD36657.1"
                     /translation="MSVHIGAEKGEIAERILLPGDPLRAKWVAENYLENVKQYNSVRN
                     MFGFTGTYKGEKISVQGTGMGLPSASIYVTELFNEYDVQVAIRIGTAGGIQDKTKVGD
                     LILAMSASTDSNINRRMTNGLDFAPHCDFKLLMAAYEASKKFERVHVGGVSSMDFFYD
                     ETDSAKKLAAHGVLALEMEASQLYSIAARKGRRALAIMTISDHVFTHEAMDSAARERT
                     LNDMVEVALHAALNG"
     gene            complement(4981..5742)
                     /locus_tag="PLW53_04205"
     CDS             complement(4981..5742)
                     /locus_tag="PLW53_04205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD36658.1"
                     /translation="MGVLDRFRLDGKVAVVTGASSGLGVAFAKALAEAGADLVLGARR
                     EERLPETGKLVEAAGRKYAYRKTDVTKPEDCDALIALAIEKFGKADILVNNAGVGTAV
                     PATRETPEAFRSVIDVNLMGAYWMAQAFAKANKDGGVIVNISSILGIKPQGLPQAAYA
                     SSKAGLIGLTRDLAAQWTGRKKIRVNALAPGFFPSEMSDELPKDMVEMLKMFTPAARL
                     GEPEELAATLIWLVSDASSYVTGITVPVDGGLVMP"
     gene            5786..6997
                     /locus_tag="PLW53_04210"
     CDS             5786..6997
                     /locus_tag="PLW53_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD36659.1"
                     /translation="MNFSLTDKASKYAEKLQLFMDQQVFPAEATYYSQREEFMKAATP
                     HKLPPVIEELKRKAKAEGLWNLFLPHLDHGLTVTDYATLAEITGWSPDIAPEAINCAA
                     PDTGNMELLDMFATPQQRKQWLEPLLAGEIRSGFAMTEPDVASSDARNIRTSIKRDGD
                     DYVINGVKWWTTGAMDERCKILIVMGKTNPEADDHHQQSMILVPIDTPGVKVVRNLTV
                     FGYVDQHGHAEVSFTDVRVPASNLLGKEGEGFALAQARLGPGRVHHCMRAIGMAERAL
                     QLMIKRSLTRSPFGKELARQGVIQEWIAKARIDIEQARLLVLKTAWIIDNQGAKAARK
                     EIAAIKVAVPKMAEEIINNAIQAHGGAGVSQDTPLAAMYAGVRTLRIVDGPDEVHLRD
                     VARLELKSYLP"
     gene            6994..8019
                     /locus_tag="PLW53_04215"
     CDS             6994..8019
                     /locus_tag="PLW53_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRD36660.1"
                     /translation="MSDLTPVRDEDAFDIGKVHTWLAPYIDQSHLPEVFQFRSGASNL
                     TYLLKYPDRELVLRRPPVGTKAVSAHDMKREFLIQSRLKPVFDLVPNVIALCEDHEIL
                     GSDFYVMERVKGEIFRRDVPETLTKEDISIMADSLVSGLAKLHFVDASVLAELNKGAG
                     YVARQVEGWSRRYRNALTDDVYDGEDVMAWLDANKPDDVGSCIIHGDWRIDNMVFDLG
                     KKKLVGVLDWELATVGDPLMDLGSALAYWVDRDDEPMFASLRRQPSHLEAMPTRREFI
                     AKYLELSNRKCDDFTFYEVFGLFRLTVIIQQIWARYRAGQTTNPAFKGFGMGVNILIK
                     RAQGLIS"
     gene            8016..8999
                     /locus_tag="PLW53_04220"
     CDS             8016..8999
                     /locus_tag="PLW53_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD36661.1"
                     /translation="MKAGRITISTRKFEVKEVPTPTPGIGEVRIKVGAAGVCLSDVHL
                     LDGTLSPGYLKGDEVTIGHEVAGTIDQLGSEVFGYQVGDRVIVIAGQRNEANQITTLG
                     FDYDGGYAEYVITKAALVVKIPDSLAFEEAAIIPDAVSTPWAAISSTAKMQAGESAAV
                     FGIGGLGIHAIQLLKIIGCSKIIAIDPRIEAREKALKVGAHFAFDPSDEGLKKHRGLN
                     AVFDFAGVASVRKQGLSLLGEMGRLVIIGIANEAITIPNDMAFTYMRTQLLGHYGSER
                     HHTQELVEMVKDGKLKLSDSVTQVLSLDQAALALETLEKKIGNPIRIVLKP"
     gene            complement(9097..10662)
                     /locus_tag="PLW53_04225"
     CDS             complement(9097..10662)
                     /locus_tag="PLW53_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD36662.1"
                     /translation="MSVPFFASSIMKGSANSHGWRHLLLDRVDNHLGQPLGAVKKSLI
                     NLIHITDTHICDAQSPARVEYLDRYADPHHPASSVIGTLIGTYRAHEVLTTQVLESAV
                     QAINRIDLAPITKKAISAVLITGDLTDNAQSNELDWLTTLLIGGKLRADSGAKDKWEG
                     VGGDFYSPFFWNPHGTPKGERDDFPRDLYGFPTIPELLNAITQPFHCSGLKYPFLAVH
                     GNHDALLQGTVAPNEELRISVKSDEKIFELPDEEALGALSKVSEQGPAAYPSPIAPKI
                     LKVSADDSRDFLTPSEWNINFYREDEDNGITSEYLGSEKRYWRKDYESLTILALDTVN
                     PFGGWQGSIDEEQFIWLKEQVEQIRDRYIVITSHHPIQDIYNGYSPSGKRILGPEIES
                     YLVTKPAVIAWICGHTHRHRIAYFGPNSQNGFYQIETSSLIDWPQQGRIIEIFINDRD
                     EICIASTVFNHYGSLLPDYEHIRLDEVNELSGLSRILSLNDWQRRGGIFAIENNEGER
                     SDRNVILTLPVRI"
     gene            complement(10662..11705)
                     /locus_tag="PLW53_04230"
     CDS             complement(10662..11705)
                     /locus_tag="PLW53_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36663.1"
                     /translation="MSKVSLKGVKKAYGKTVALEHLNLELEQGSLVTLLGPSGCGKTT
                     ALRILAGLTKEDSGQVFFDSIDITKTSPADRDIGMVFQAYSLFPNMTARQNVEFGLRT
                     RRVNKSEIESRVKKIFEIIELGSHIDRYPHQLSGGQQQRVALARAIVVRPKVLLLDEP
                     LSALDAQVRDQLRDEIKRVQREFGITTLFVTHDQEEAMTISDRVGVMDKGRLLQIDTP
                     QKVYAQPINPTVARFIGIMNEVPAVVSGNTVATLGSNFSLALESSFAPGSKNLKALIR
                     PEAIEIIEPNHIGVPGKIISISFLGPIAMVQISLDRNLEIRAILPAAMINRYQVDQVV
                     HVTSSAKSLIVAD"
     gene            complement(11722..12504)
                     /locus_tag="PLW53_04235"
     CDS             complement(11722..12504)
                     /locus_tag="PLW53_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD36664.1"
                     /translation="MAALKIIGSAFRILVIVTGISFFMLPVFAASNFAFEDGQGGYTV
                     TPIRSAAGNQELMQSLLLTMQIAFGTVLLTLLLLIPTVAYLHLVIPGARAWVEFLTLT
                     PLVIPPIVQAVGFLYSMPTWLKSTPNSLAFAYVILALPFSYRALDAAFATIDARTLYD
                     ASRSLGASFSAAIRKVLIPNVATGIYGAIFLTIALVLGEFAYASLLLWDTFPTELAVA
                     GMANASTAVALSVLSLLGVWLILIGINLFNRKSKTTVVAGAK"
     gene            complement(12492..13334)
                     /locus_tag="PLW53_04240"
     CDS             complement(12492..13334)
                     /locus_tag="PLW53_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD36665.1"
                     /translation="MLKRAGLIGLAPLLIYMAMFLGLPLTAILRFSFLNSENEFTLNN
                     FATISSGIYRDGFILSSQLSLVSAALSLIFGFLFAYALATSKWNYLKKLVSTASGVLA
                     NTGGVPLAFMFIAVLGTYGALTTFLKEFGIELYGGSWTLYSFSGLLIVYLYFQVPLMV
                     LICLPVLENIKVEWRDAASSLGANNLTYWRKVLIPILTPQLLGFFLLLFANSFAAYAT
                     AAALTSGSLALVPVQIASLISSNVQVDGANTAMALGLGMVVLVGVSTIGYALLEKRTS
                     QWRR"
     gene            complement(13435..14562)
                     /locus_tag="PLW53_04245"
     CDS             complement(13435..14562)
                     /locus_tag="PLW53_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD36666.1"
                     /translation="MKKKSLRAVVGIVLALALSSNFAIAQAATKKPAQAQSAADVGGL
                     KELIKLAKKEGELNTIALPDYWANYGNVIKRFSEKYGIKVNSYDPEASSAEELVAVRT
                     LKGQDRMPDVLDVGLQFAVQGAQEGLLAPYKVIGWKDIPDAAKDPKGLWYSDYGGYIA
                     IGYDANIVKTPPKSFADLLNPIYKNQVALNGDPTASNSAFLSVMATSVAAGGGVKGAN
                     NINFALDYWKQMKASGNFVPVAATPATVAAGQTPILLDWDYLHAKYRDQSGKVDWKVV
                     VPSDGLLGGFYAQAIVKNSPHPAAARLWQEFLYSDEGQNLWLQGGARPIRLDAMVKAN
                     THNKTAYALLPATPKNAKPVFPSLQQQLLAKALLTPAWPKL"
     gene            complement(14702..18529)
                     /locus_tag="PLW53_04250"
     CDS             complement(14702..18529)
                     /locus_tag="PLW53_04250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF011498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRD36667.1"
                     /translation="MLDVNFFDELRIGLASAGNIREWSFGEVKKPETINYRTLKPEKD
                     GLFDEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYMITEVDVEAREEDLPKLEKKMNSDRK
                     KIETKRDNDLALRQEKMENDLAELEDEGAKADQRRKVREAGERELKNIRERSQKELDR
                     LEEVWTRFKNLKVQDLEGDENLYREMRDRYGIYFKGYMGAAAIQRRLETFDLQAEFDK
                     LNVLAETGKGQKKTRAIKRLKVVNSFLTTSNKPASMVLDCVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLVDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALE
                     LFKPFVMKRLVDLNHAQNIKSAKRMVERSRSVVWDVLEEVIAEHPVLLNRAPTLHRLG
                     IQAFEPQLIEGKAIQIHPLVCTAFNADFDGDQMAVHVPLSAEAQAEARILMLSSNNIL
                     SPASGRPLTAPTQDMVLGLYFLTSLRENQLGEGRSFTSISEGLMAYEQNSLSLQAKIK
                     IRVNINGELVTKETTLGRAIFNEVLPESFPYVDYDVTKKALGLIVDNLAEGYPKVVVA
                     AVLDALKELGFTWATRAGATIGIVDVVTPGRKLEILEGYETKADKVQSQYEKGLITDD
                     ERRQELIEIWTQATNEVAKEMEDNFPRTNPVWMMVFSGARGNMMQIRQIAGMRGLVAN
                     PKGEIIPRPIKSNFREGLSVLEYFISTHGARKGLADTALRTADAGYLTRRLCDVAQDV
                     IIREEDCGTDRGLTLKIGNIVAGKLTRDDYVETSIYGRTLADDVVVDGKTIVTSGADL
                     GDRLINELIDAGVSEIKVRSILTCDSKVGQCAKCYGRSMASGKLVDVGEAVGIIAAQS
                     IGEPGTQLTMRTFHTGGAATASGDITHGLPRVVELFEARTPKGVAPIAETAGVVSFLE
                     DAKGKKIIVTPEDGSEAVAYPITRRQKLLVEDGQKVSVGQQMVVGAIDPKQVLKILGA
                     RSTQTHLVHEIQEVYRTQGVGIHDKHIEIVVKQMLRRITVLEPGDTDLLPGELVDHLR
                     FQSANREAVQKGGKAANGRPELMGITKASLATESWLSAASFQETTRVLTDAALSEKSD
                     PLLGLKENVIIGKLIPAGTGLARYRNVRVEPTEEAKAAVYAAYEEYDYAPFDTTGSGE
                     AVRLDDYQVGENR"
     gene            complement(18547..21999)
                     /gene="rpoB"
                     /locus_tag="PLW53_04255"
     CDS             complement(18547..21999)
                     /gene="rpoB"
                     /locus_tag="PLW53_04255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRD36668.1"
                     /translation="MAAKTKSTAPNRISFAKIREPQEVPNLLALQIESFDWLLGNDAW
                     RARLGKNERPDRGEIPKTSGLEEIFEEISPIEDFQGSMSLSFRDHRFEPSKYTIAECK
                     ERDITYAAPLFVTAEFTNNVTGEIKSQTVFMGDFPLMTPRGTFVINGTERVVVSQLVR
                     SPGVYFERTVEKASDKDIFTAKIIPSRGAWLEFEIDKKDFVAVRIDRKRKQSVTIFLK
                     ALGWSNDQILAEFGEYESIKETLAKDNVSTQDEALLDIYRKMRPGEPPTKEAAQTLIE
                     NLYFNPKRYDLAKVGRFKVNKKLGLDLDLGKSLLAIDDIVGAIRYIAALHKGETLIDL
                     GKQVRVEIDDIDHFGNRRLRTVGELIQNQVRVGLSRMERVVRERMTTQDVEAITPQTL
                     INIRPITASIKEFFGTSQLSQFMDQTNPISGLTHKRRLSALGPGGLSRDRAGFEVRDV
                     HPSHYGRMCPIETPEGPNIGLIGSLATYARITPFGFVETPYRKVSKGKVTDAVDYLTA
                     DEEDEHIIAQANAPLTEDNHFAEARVLVRRRGGEVEYIPAEEVDYMDVSPRQMVSVAT
                     AMIPFLEHDDANRALMGANMMRQAVPLLRAESPLVGTGMEYRAAVDAGDVLLAVRGGV
                     VSEVASDLIVIKTDDGEEDTYNVDKFIRSNQGTSRNMRVIVSQGQKIAAGQAIADGPS
                     TELGEMALGKNLLVAFMSWEGHNYEDAIILSQRLVQDDVLSSIHIEEYEVDARDTKLG
                     AEEITRDIPNVSEEVLADLDERGIIRIGADVVPGDILVGKVTPKGETELTPEERLLRA
                     IFGEKAREVRDTSLKVPHGEGGKVIGVKIFDLDDGYDLAAGVNQVVRVYVAQKRKIQD
                     GDKLAGRHGNKGVISKILPVEDMPFLADGTPVDVVLNPLGVPGRMNVGQVLETHLGWV
                     AKSGWQVSGSDKGWQERLGAIGALSAEPGTRVATPVFDGAREEEITGLLGSTLPNADG
                     DRLVGEQGKAILFDGRTGQPYREPITVGYMYILKLHHLVDDKIHARSTGPYSMITQQP
                     LGGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTIKSDDVLGRVKVYEAIVKGENIPE
                     PGIPESFKVLVKEMQSLCLNVEVLSSEGVAIEMRDTDEEVFKTAEELGINLSRVEPSS
                     VEEV"
     gene            22249..22518
                     /locus_tag="PLW53_04260"
     CDS             22249..22518
                     /locus_tag="PLW53_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRD36669.1"
                     /translation="MEKILSKRAVAISEFKRAPNEIVAEANGEPLAVLTNNKPSFYVI
                     SPSAYEELLEQLWELEITPLLAKRLSDLKSGKSKSIQVTIEDLIK"
     gene            22515..22820
                     /locus_tag="PLW53_04265"
     CDS             22515..22820
                     /locus_tag="PLW53_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD36670.1"
                     /translation="MKYQLRFLSEAWDEWKELDKSIKIPLARKLEKRLEDPKIASAQL
                     HGKLIGLYKIKLSDSGYRLVYQVLDKEVVVLVISVGRRDKSKAYDLAQERIKKAPPR"
     gene            complement(22848..23234)
                     /gene="rplL"
                     /locus_tag="PLW53_04270"
     CDS             complement(22848..23234)
                     /gene="rplL"
                     /locus_tag="PLW53_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRD36671.1"
                     /translation="MAKLSSTDLLAQFKEMTLVELSDFVKAFETEFDVTAAAPVAVAA
                     AGGAAGGGEAAAAQDEFTVVIEEAGSQKIAVIKEVRALNSSLGLKEAKDLVDSAPATV
                     LEKADKATADKAKAALEAAGAKVSIK"
     gene            complement(23253..23918)
                     /gene="rplJ"
                     /locus_tag="PLW53_04275"
     CDS             complement(23253..23918)
                     /gene="rplJ"
                     /locus_tag="PLW53_04275"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRD36672.1"
                     /translation="MARSDKAASVAELVEDFKSADATVVTEYRGLTVTAIKELRRSLG
                     VGTKYKVAKNTLVKIAAKEAGVEVADSLLTGPTAVAFVQGDSVAAAKSLKNFQKENPF
                     LIIKGGIFEGKAVTTAEIMKLADLESREVLLAKLAGAMKASMAKAARAFDALRIKREA
                     ESGAPAAPEQAAPAAEAAPVAEATTQEVVAEAAPVAEATNEEVVAEAAPETSAPVEPT
                     ENA"
     gene            24261..24331
                     /locus_tag="PLW53_04280"
     tRNA            24261..24331
                     /locus_tag="PLW53_04280"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24293..24295,aa:Glu,seq:ttc)
     gene            24516..>24834
                     /locus_tag="PLW53_04285"
     CDS             24516..>24834
                     /locus_tag="PLW53_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36673.1"
                     /translation="MDRADLQNYLFLNPLGHFGLTAVTFLTLLPLTQVIVVFLATAAL
                     VGVGAAIVGVGDGEATADSVGVGVGVGVGVGVGVGVGVGVGVGVTTAAGVNLNTPRLT
                     INVC"
BASE COUNT         6717 a         5724 c         5418 g         6975 t
ORIGIN      
        1 ttacgatgcg agcaccggct cgctcatcca tggcctggat ttggccacga cggctattga
       61 gatcgccgat gacatcgccc atgaaatctt caggggtggt gacttcaacg ctcatcattg
      121 gctcgagcaa taccggaccg gcaagttttg ccgcttcctt aaatgctgcg ataccagcaa
      181 tcttgaaggc caactccgat gagtcaacat cgtgataagc gccatcaaga agagttactc
      241 gtacatcagt taatggatag ccagcaagtg ggccattttg tagcgcctct tggcaaccat
      301 catcaacaga agggatgtac tccttaggga tacgaccacc ggttacttta tttacgaatt
      361 catatccgcc ttcaacttcg ccaactggaa gtggctcaag tgcgatctga atctttgcaa
      421 actgtccaga tccaccggtc tgcttcttat gggtgtagtc atagcgagca actggctttc
      481 ttagagtctc gcgataagca acttgtggct ttccaacatt tgcctcaact ttaaattcac
      541 gcttcatacg atcaacaaga atttctagat ggagctcgcc cattccacca atgatggtct
      601 gtgcggtctc ttcatcagtt ctaacatgaa atgttggatc ttcttcagca agtctttgaa
      661 tcgcaacgcc aagtttttct tgatcgccct tggtctttgg ctcaatcgca acgtggataa
      721 ctggagctgg gaaatccatt gactccaaga tcactggctt atcaatatcg cagagcgtat
      781 ctccggtggt ggtgtcctta agacccatca cagcgatgat atctccagct gatgcctgag
      841 gaatttcttc tctcttattg gcatgcatgc gatagatctt gccgatgcgc tctttacgat
      901 cttttgtgga gtttagaacg ccgcttccag cctctaatac acctgagtaa attcttacga
      961 aagtcagctt tccaaggtgt ggatcgctca taatcttaaa agcaagtgat gaataaggct
     1021 cttttggatc tggctttcta tcaatttcaa cgctcttatc acccatcttg gttcccttaa
     1081 tcgcctcaac atcaagaggt gatggcagat agcgagtaac ggcatcaagc attggttgaa
     1141 cgcccttatt cttaaaggca gaaccagtta gaactggagt taacttataa gcaagagttg
     1201 ctcgtctaat tccagcgata atttcatctt cacttatagc tgcgccctct aggtatttct
     1261 ccataattgc atcatctgca tcagagagag tttcaatcag agcaaaacga gctgcatcac
     1321 atttttcttt taggtctgca ggaatttctt caattacata atcttcaccc ttttgggtct
     1381 cacctctcca ggtgagggcc ttcatagcaa ttagatcgac aactccaata aagtcacctt
     1441 caactccaat tggaacttga agcacaagag cggttgcgcc aagacgcgaa ccgatcatct
     1501 caacgcagcg atcaaagttt gcgccggtgc gatctagttt gttaacaaaa caaatacgag
     1561 ggacgctgta cttatcagct tggcgccaga cagtttcaga ttgtggttca acgccagcta
     1621 cgccatcaaa aacagttact gcgccatcta gaactcgaag tgatctctca acttcaactg
     1681 tgaagtcaac gtgaccaggg gtatcaatga tgttgatggt gtggccgcgc cactcgcaag
     1741 ttgtagcagc tgaggtaatt gtgatgcctc gctcttgctc ctgctccatc cagtccatgg
     1801 tggctgcgcc ctcatgaacc tcgccaattt tgtagttaat accggtataa aaaaggatgc
     1861 gctctgtggt ggtggtttta ccagcatcga tatgggccat gatgccgata tttcgcacct
     1921 tggctagatc aattgcagtc gctgtcgaca tattaattac cacctatagt gtgcaaaagc
     1981 cttattagct tctgccatct tgtgggtgtc ttcacgcttt ttaactgcag cgccgaggcc
     2041 attggaggca tcgatcattt catttgctaa ccgctctgcc attgattttt cacgacgtag
     2101 gcgagcgtta tcaactaacc aacgaaggcc aagtgtcata gagcgagcag ctttaacttc
     2161 aaccggaact tgatatgtag cgccgccaac tcttcgtgat ctaacttcaa cagctggctt
     2221 tacattatct agagctttct taagagtaat aactggatct acctcagtct tagcgcggca
     2281 attttctagc gctttataaa caatagcctc tgaagttgag cgcttgccat gaactaaaca
     2341 cttattaata agtgcggtga caactggaga gttatatacc ggatcggcaa taactggaga
     2401 tttagcagca gggcctttac gtggcattac ttcttctcct tcttagcgcc ataacgagag
     2461 cgagattgtt tacgatcttt aacaccttga gtatcaagag aaccgcgtac tactttgtaa
     2521 cgaacaccag gaagatcttt aacgcgacca ccacgaacta aaacaatgga gtgctcttgc
     2581 aagttatgtc cttcaccagg aatgtatgcc gttacttcca taccgctagt tagacgaaca
     2641 cgtgcaactt ttcttaaagc tgagttcggc ttctttggcg ttgtggtgta aacgcgtgtg
     2701 cagacgccac gacgttgcgg acttccttta agacccggag ttgttgtctt atctgactta
     2761 tcaacccggc ccttacggac caactgctgg attgttggca ctacttcatc tccatctcgc
     2821 ttatccgacc cacgcggtcg ggtgtgtcgc tactttgagc gcaagggcgc gaggttatag
     2881 gaaagatcta ctgcaggtca aaacgggtaa atcagccaaa cacgccgatt tttgggggca
     2941 acacgccgag caaaatagca ctagccttta cttatggcag gcaaagtaaa ggctaagaag
     3001 gcaggcaaga aatccacaat taagaacccc cggcgcgggc gggccactac ttctcggata
     3061 agttccaaaa accagataac tattccagtg gaggtgcttc gagaggtgaa tttaaaacct
     3121 ggagataaag tggaattcat gattgataaa caagagcgaa taattcttgc accgagtgat
     3181 caagcaagtt ggaaaaagaa tttaatgcag ctggctggaa gtatgccaga gcttggttcg
     3241 aattttgatt ataaaaagga gcgccaagag tgggacttaa aagccacgca gacacctctt
     3301 ggttaattgc gctactaaat cctcttgata tctctcacaa attagccatc cgccaacttg
     3361 aagagctaaa tgccccagcc tcgattagca cctttgtctt agctgaacta atggtcagct
     3421 ttgaaaaaag cgatattgga agtatttcgg tgactctccg agagattgaa aaatctttct
     3481 caccaattgt agagctaagt aaagaaattg ccattagtgc tgcacagatt cgttcaagta
     3541 gcaagataac cttgggtgat gcaattgtta ttgcttccgc tctcgatcaa aatagcgagc
     3601 ttcttacctt cgataagaat atgaaagcag tctatgaacg tattaaatag accaaagctg
     3661 cgcggcatta ttcatttagt tatgtctcca cttgcactag ttgctggcct ggttttaatc
     3721 acaatcacaa ctgagcttcg cggaagagtt acgctaacaa tatttactct cactgcagtc
     3781 tcgctattta cttgtagcgc gatctatcac cgcgtgcctt gggggccttc tgccaaagca
     3841 atctggcgca gaattgatca tgccaatatt ccttttctaa tagctggcac ctacacccct
     3901 tttgcgatct atctattaga taagagccaa gcggtaatac tgctctcgct ggtctggggc
     3961 ggggccctgc tctctggagt atttagagtc ttctggctta acgctccgcg ctggctctat
     4021 gtgccgatct atctagcact tggttgggcc gcactcattt atcttcctga cttctatcga
     4081 aatggcgggc ttgcagtcct tctacttatt gcctttggcg gaattctcta ttcactgggc
     4141 gcgattatct atgccgttaa gtggcctaat ttttctatca actggtttgg cttccatgag
     4201 ttatttcacg ccatgactgc tgctgcattc atctcacact ttattgctgc aattctggtt
     4261 atagttggct aactatgagc gtacatatcg gagcagaaaa aggcgaaatc gccgagcgaa
     4321 tccttcttcc tggagatcca ttaagagcta agtgggttgc tgaaaattat ctagaaaatg
     4381 ttaagcaata taactcggta agaaatatgt ttggctttac tggaacatat aagggcgaga
     4441 aaatatcagt gcaaggaact ggcatgggac ttccctctgc ctcaatctat gtcactgagc
     4501 tctttaatga atatgatgtt caagtcgcca ttcgaattgg cactgcaggc gggattcaag
     4561 ataaaactaa agttggcgat ttaatccttg ctatgagcgc atcaactgat tccaatatca
     4621 atagacgcat gaccaatgga cttgattttg ccccgcattg tgatttcaaa ttacttatgg
     4681 ctgcctatga agcatcaaag aagtttgagc gagttcatgt cggaggggtt tcatcgatgg
     4741 actttttcta tgatgaaaca gattctgcta agaaacttgc agcccatggc gttttagcac
     4801 ttgagatgga ggctagccag ctctactcaa ttgcagctag aaaagggcgc cgggcgcttg
     4861 caattatgac tatctcagat catgtcttta ctcatgaagc tatggattca gctgctcgcg
     4921 agcgcacctt aaacgatatg gttgaagtcg ccctccatgc cgcgcttaac gggtaatttc
     4981 ttaaggcatt actaagccgc catcaactgg aactgtaatt ccagtgacgt agcttgaggc
     5041 atcacttact aaccaaatta gagttgctgc tagctcctct ggctctccaa gacgagccgc
     5101 tggggtaaac atctttaaca tctcaaccat atcttttggt agctcatcac tcatctctga
     5161 tgggaaaaat cctggcgcaa gtgcatttac gcgaatcttc tttcgcccag tccattgcgc
     5221 tgccagatct cgagttaaac caattagccc cgctttactt gaagcatagg cagcttgtgg
     5281 aagtccttga ggtttaattc caagaatgct tgagatattt actattactc cgccatcttt
     5341 atttgcctta gcaaatgctt gagccatcca ataagccccc atcaaattaa catcaattac
     5401 acttctaaat gcctctggag tttccctagt tgctggcact gcagttccaa cgcctgcgtt
     5461 attaaccaag atatctgctt tgccaaattt ctcaattgca agagcaatta gcgcgtcgca
     5521 atcctctggc tttgtgacat ctgtttttcg ataggcatac tttctgcccg cagcttctac
     5581 taactttcca gtttcaggca atctctcctc gcgcctagcg cctaagacaa gatccgctcc
     5641 agcctcagcg agtgctttag cgaaggcaac tccaagacct gatgaagctc cagtaacaac
     5701 agcaaccttg ccatcaaggc gaaagcgatc aagaacaccc attttccctc tcctaatttc
     5761 gagcaagtat gcgtacggta taggcatgaa ctttagcctc actgacaagg ccagcaaata
     5821 cgccgagaaa cttcaacttt tcatggatca gcaggtcttt ccagccgagg ccacttatta
     5881 cagccagcgc gaagaattta tgaaagcggc aacgccacat aagttgccac cagttattga
     5941 agagctaaaa cgtaaagcaa aagctgaggg gctatggaat ctcttcctgc ctcatcttga
     6001 ccatggttta actgttacag attatgcaac ccttgctgaa attactggtt ggtcgccaga
     6061 tatcgcacca gaggcaatta attgcgcagc tcctgatact ggaaatatgg aattgctaga
     6121 tatgtttgca acaccgcagc agaggaagca atggcttgag ccgctattag caggtgagat
     6181 tcgctctggc tttgccatga ccgagcctga tgttgcatca agtgatgcaa gaaatattag
     6241 aactagcatt aagcgagatg gtgatgacta tgtaataaat ggcgttaagt ggtggaccac
     6301 tggcgctatg gatgagcgat gcaagatttt aattgtgatg ggaaaaacta atcctgaagc
     6361 agatgatcat caccaacaat cgatgatatt ggttccaatt gatactccag gagttaaagt
     6421 tgttagaaac ttaactgtct ttggatatgt tgatcaacat ggccatgccg aagtttcatt
     6481 tacagatgta agagtgcctg caagtaatct tcttggcaaa gaaggggaag gttttgctct
     6541 tgctcaagct cgtcttggtc cgggccgggt ccatcactgc atgcgagcta ttggaatggc
     6601 agagcgagcg ctgcagttaa tgattaagcg aagccttact agatctccct ttggaaaaga
     6661 gttagctcgc caaggagtaa ttcaagaatg gattgcaaag gcgcgtattg atattgagca
     6721 ggctcgccta cttgtcttaa aaacagcgtg gatcattgat aatcaagggg ctaaagctgc
     6781 gcgcaaagaa attgctgcta ttaaagtagc ggtgccgaaa atggcagagg agataatcaa
     6841 taatgccatt caggctcatg gcggagcagg tgttagccaa gatactcccc ttgcagctat
     6901 gtatgcagga gttcgaactt taagaattgt tgatggacca gatgaagtgc atctacgcga
     6961 tgttgcaagg ttagagctca agtcctatct gccatgagtg atctcactcc agtaagagat
     7021 gaagatgcat ttgatatcgg caaagttcac acctggcttg cgccatatat agatcaaagt
     7081 cacttgccag aggtttttca atttagatct ggcgcatcaa atctaactta tctacttaaa
     7141 tatccagatc gtgagttagt ccttcgccgt cccccagttg gaacaaaagc agtctctgcc
     7201 catgatatga agcgagaatt tctaattcaa tctcgtctaa agccggtatt tgatttagta
     7261 cccaatgtaa ttgcgctctg cgaggatcat gaaattctcg gctctgactt ctatgtaatg
     7321 gaaagagtca agggcgaaat atttcgaaga gatgttccag agacgctaac aaaggaagat
     7381 atttccatca tggctgactc tctagtttct ggccttgcca agcttcactt cgtagatgcc
     7441 tctgtcctag cagaactaaa taagggagca ggctatgtag ctcgccaagt tgagggttgg
     7501 tctagacgat atcgaaacgc actaactgat gatgtttatg atggcgaaga tgtaatggcc
     7561 tggcttgatg ctaataaacc agatgatgtt ggctcatgca tcatccatgg tgattggcgg
     7621 atagataaca tggtttttga tttaggcaag aaaaagctag tgggagtgct ggattgggag
     7681 ctagcaacag ttggcgatcc gcttatggat ttgggctctg cccttgctta ttgggtcgat
     7741 agggatgatg aaccaatgtt tgcctcactt cgccgtcaac ctagccatct tgaagcaatg
     7801 cctactcgaa gagaatttat tgcaaaatac cttgagctaa gtaatagaaa gtgtgatgac
     7861 ttcacattct atgaagtctt tggcttattt agattaacag tgatcatcca acaaatctgg
     7921 gctcgatata gagcaggtca gactacaaat cctgccttta aaggtttcgg catgggagtt
     7981 aacattttga ttaagcgtgc gcaaggattg atctcatgaa agctggacgg atcacaattt
     8041 caactagaaa gtttgaagtt aaagaagttc caactccaac tcctggtatt ggcgaggttc
     8101 gcataaaagt aggcgcagct ggagtctgtc tctctgatgt tcatttactt gatggcacgc
     8161 tctcgccagg atatctaaaa ggtgatgaag taactatcgg ccatgaagtg gcaggaacaa
     8221 ttgatcaact cggtagtgaa gtatttggat accaggttgg agatcgagta attgttatcg
     8281 ctgggcagcg taatgaagca aatcaaatta caacactagg ttttgattac gatggtggtt
     8341 atgccgagta tgtcattact aaggcagcgc tagttgtgaa gattcctgac tcacttgcct
     8401 ttgaagaagc tgccatcatc cctgatgcag tatcaactcc ctgggctgca atctcctcaa
     8461 ctgcaaagat gcaagcaggt gagagcgctg cagtatttgg aattggcggc cttggaattc
     8521 acgcaatcca gcttcttaaa atcataggtt gtagcaaaat aattgccatt gaccctcgga
     8581 tagaggcccg tgaaaaagcc ctaaaagtag gggcccattt cgcctttgat ccaagtgatg
     8641 aagggctaaa aaaacatcgg ggcctaaacg ctgtctttga ttttgcagga gtggcatcgg
     8701 taagaaagca ggggctctca cttcttggcg agatgggtag attggtaatt attggaattg
     8761 ctaatgaagc catcactatt ccaaatgata tggcctttac ctatatgaga acccaactcc
     8821 taggacatta tggatctgag cgccatcaca cccaagaact tgtcgagatg gttaaggatg
     8881 gaaaattaaa gctctctgat tcagttactc aagttctctc acttgatcaa gcagcccttg
     8941 cgctagaaac acttgagaaa aaaatcggca acccaatccg tattgttcta aaaccgtagc
     9001 aaagtctagc aatatatttt tcggggtttt ctaaatctta ttaactagca gttcacttca
     9061 taaagttgaa aagtcacttg atgcaacctt tactggctag attcttactg gcagagtaag
     9121 aatcacattt cgatcagagc gctctccctc attattttca attgcaaaaa ttccaccacg
     9181 cctttgccaa tcattgagcg agagaattcg cgatagaccg gagagttcgt taacctcgtc
     9241 aagccttata tgttcatagt caggaagtag cgagccgtag tggttaaaga cagtgctggc
     9301 aatgcaaatc tcatctctat cgtttatgaa gatctcaata attcgtcctt gttgtggcca
     9361 atcaattagt gatgaagttt cgatttgata gaacccattc tgactattcg gaccaaagta
     9421 ggcgatacga tgccggtgag tatggccgca gatccatgcg atgacagcag gcttggttac
     9481 tagatagctc tcaatttcgg gccctaaaat gcgttttcct gatgggctgt acccattgta
     9541 aatatcttga atcggatgat ggctagtaat tacgatatat cgatctctta tttgctctac
     9601 ttgctctttg agccaaataa attgttcctc atcaattgag ccctgccaac caccaaatgg
     9661 attcactgtg tctaaggcaa ggatggtcaa tgactcatag tcttttctcc aatacctttt
     9721 ttcgctacct aaatactcac ttgtaattcc attatcttca tcttcacgat aaaaattgat
     9781 gttccattca cttggagtga gaaagtccct agagtcatca gcgcttactt tcaaaatctt
     9841 tggagcgatt ggagatggat aagcggccgg tccttgttcc gatactttag aaagcgcccc
     9901 cagcgcttcc tcatctggca gctcaaaaat cttctcatct gactttactg atattcgcag
     9961 ctcttcattt ggggcgacag tgccttgaag gagagcgtca tgatttccat gtactgctag
    10021 gaaaggatat ttgagacctg agcagtgaaa gggttgagtg atggcattta agagctctgg
    10081 aatcgtggga aaaccatata gatctcgcgg gaaatcatct ctttcgcctt ttggtgttcc
    10141 atgtggattc cagaaaaatg gcgagtaaaa atctcctcct actccctccc acttatcttt
    10201 agcgccacta tctgctcgaa gttttccgcc aattaataaa gtagtgagcc aatctaattc
    10261 attagattgc gcattatcag tcaagtctcc tgtaattaat acagcactaa tggctttttt
    10321 tgttataggt gctaaatcaa tccgatttat tgcctgcact gcactctcca gcacttgcgt
    10381 cgtcaaaact tcgtgagccc gataagttcc aattaaagtt ccaattacac ttgatgctgg
    10441 atgatgtggg tctgcataac ggtccagata ttcaactctg gcaggtgatt gagcatcgca
    10501 gatatgtgta tcggtgatgt gaatcagatt tattagagac ttctttactg cgcctaatgg
    10561 ttgacctaaa tgattatcta ctcgatctaa gagtagatgt cgccatccat ggctatttgc
    10621 agagcccttc attattgagc ttgcgaagaa tggaacagac attagtcagc aacaataaga
    10681 gatttggcag atgaggtgac atgaaccact tgatcaacct gatagcgatt aatcatggca
    10741 gcaggtaaaa ttgcccgaat ttcaaggttg cgatctagtg aaatctgcac catggcaatc
    10801 ggaccaagaa atgaaatact gataattttt ccaggcactc caatatgatt cggttcaata
    10861 atttcaattg cctcaggacg aatcaaagcc tttagattct tacttcctgg cgcaaagctt
    10921 gattccagtg ccaagctaaa gtttgagccc agtgtggcaa cggtatttcc ggaaacaact
    10981 gcaggaacct cattcatgat cccaataaac cttgcaactg ttggattaat tggctgagca
    11041 taaacttttt gtggggtatc gatttgtagc aatctacctt tatccatcac accgacacga
    11101 tctgaaatcg tcatcgcctc ctcttgatca tgggtgacaa ataatgtggt aatcccaaat
    11161 tctcgctgca ctcgcttgat ttcatctctg agttgatctc taacttgggc atcaagggct
    11221 gagagtggct catctaaaag caaaactttt ggacgaacaa caatcgctcg agccagcgct
    11281 actcgctgtt gctgccctcc agagagttgg tggggatagc gatcgatgtg gctacctaat
    11341 tcaatgattt caaagatctt ctttactcga gattcaatct cacttttatt taccctcctg
    11401 gtacgtagac caaattcaac attctgacga gcagtcatgt ttggaaagag actataggct
    11461 tgaaatacca ttccaatatc acgatctgct ggagatgtct ttgtaatatc gattgaatca
    11521 aaaaagactt ggcctgaatc ctcttttgta agtcccgcta atattcttag ggcagtagtt
    11581 ttgccacagc cggatggtcc aagcagagta actagtgagc cttgctctaa ctcaagattt
    11641 aaatgctcaa gggcaaccgt ctttccataa gctttcttta caccctttag gcttaccttt
    11701 gacacttttt ctctcccatc gttattttgc tcctgcaact actgttgttt tgctctttcg
    11761 attgaataga tttatcccaa ttaaaatcag ccacacgcca agcaagctaa gaactgagag
    11821 tgcaacagct gttgaggcat ttgccatgcc ggcaactgct agctctgttg gaaatgtgtc
    11881 ccagagcagc agtgaggcgt aggcaaattc acccagaacc agagcgattg ttagaaagat
    11941 tgcgccatat attccagttg ccacatttgg aatcagaact tttcgaatcg ctgcgctaaa
    12001 gcttgctcct aaagatcgag aggcgtcata gagggtgcgg gcatcaattg tggcaaatgc
    12061 ggcatcaagg gcccgatagc taaagggaag agccagaatt acataggcaa aagctagtga
    12121 attcggggtt gatttgagcc aagttggcat ggaatataga aatccaacag cttgcacaat
    12181 gggtggaatc actaatggcg ttagtgtcag gaattccacc catgcacgtg ccccagggat
    12241 gacaaggtgt agatacgcaa ctgttggaat gagcaaaagt agcgtcaata acaccgtgcc
    12301 aaaagcgatt tgcatagtaa gtagaagtga ttgcattaac tcttgattgc cagctgcact
    12361 ccttattgga gttacggtat agcctccctg gccatcttcg aaggcaaaat tagaagctgc
    12421 gaatactggg agcataaaaa atgaaatgcc agtaacaatt accaatatcc taaatgcgct
    12481 accaatgatt tttagcgccg ccattgagaa gtcctcttct ccaaaagggc ataaccaata
    12541 gttgagactc caaccaaaac caccatacca agaccaagtg ccatcgcagt atttgcgcca
    12601 tcaacctgga catttgagct aattaaggat gcaatctgaa cgggcacaag cgcaagtgat
    12661 ccgctggtga gggctgctgc tgtggcgtat gctgcaaaag aattggcaaa gagcaaaagg
    12721 aagaagccca atagttgtgg ggtaagaatt gggattaata ccttgcgcca ataagtcaga
    12781 ttatttgcgc caagagagga tgctgcatca cgccattcca ctttgatatt ttccaaaaca
    12841 ggtagacaaa tcaaaaccat caaaggaact tggaaataaa ggtaaacgat caataacccc
    12901 gagaaggagt agagcgtcca acttccgccg tagagctcga taccaaattc cttcaagaat
    12961 gttgtcaaag ccccataggt tccaaggact gcaataaaca taaatgccaa cggcactccg
    13021 ccagtatttg ccagtactcc cgatgctgtt gagactagtt tttttaagta attccacttt
    13081 gaggttgcca gcgcataggc aaaaaggaag ccaaaaataa gtgataaagc agctgaaacc
    13141 aatgataatt gtgagcttaa gataaagccg tcccgataaa ttccactact tattgtggca
    13201 aagttattta gagtgaactc attttctgaa tttagaaatg aaaaccttaa aattgcagta
    13261 agtggcaggc ccagaaacat tgccatataa atcaaaagcg gggctaagcc gattagcccc
    13321 gctcttttaa gcatctaacg cccttcatct atcaagcaga tttacagaat catttgtgta
    13381 gcgaaagttg gaccctcgaa ccaactttcg ctactcaaac tttttctagc agtgttacaa
    13441 cttaggccaa gcgggagtta acagagcctt agctagaagt tgctgctgca aggatgggaa
    13501 gaccggcttt gcgttttttg gcgtagctgg cagtagcgcg taggcagttt tattgtgggt
    13561 attggctttc accattgcat caaggcgaat cggacgagcg cctccctgca accacaagtt
    13621 ctgaccttca tcgctatata ggaactcttg ccacaaacga gctgcagcag gatgcggaga
    13681 gttcttcacg atagcttgtg cgtagaaccc accgagcaag ccatcagatg gaacaacgac
    13741 tttccaatca accttgcctg attggtccct gtacttggca tgtaggtaat cccaatctag
    13801 gagtattggg gtttgacctg ctgccacagt tgctggagtc gctgcaacgg gaacgaaatt
    13861 tccagaagcc ttcatctgct tccagtaatc aagagcgaaa ttgatgttat tagcgccctt
    13921 tactcctcca cctgcagcga cggatgttgc cattactgag aggaatgctg agttggaggc
    13981 agttggatcg ccattaagcg ccacttgatt tttgtagatt gggttcaata aatccgcgaa
    14041 tgactttggt ggcgtcttta caatatttgc gtcatatcca attgctatat atccgccgta
    14101 atctgaatac caaagaccct ttggatcttt agcagcatct ggaatatctt tccagccaat
    14161 gactttataa ggagcaagaa gtccttcttg tgcgccctga actgcaaatt gcagacctac
    14221 gtccaagaca tcgggcatgc gatcttgacc cttaagggtg cgaactgcca ctaactcctc
    14281 agctgagctt gcctctggat cataggaatt taccttaatt ccatacttct cagagaagcg
    14341 cttgattaca ttgccataat ttgcccagta atcaggaaga gcaatggtat ttaactcgcc
    14401 ctctttttta gcaagtttga ttaattcttt caatccacca acatctgcgg cagattgagc
    14461 ttgtgcaggc ttcttagttg ctgcttgcgc gattgcaaaa ttagaagata gcgctagtgc
    14521 tagcacgatg ccaactacag cgcgtaatga tttctttttc atgcgactcc taacgtcggt
    14581 gtggggagtc aagttgtaga gaattaattt agaatagatt cattctttca atcagtagaa
    14641 tatttggtaa acaaaaatcc ccgatactct aattagtatc ggggatttaa ccttctgtta
    14701 actagcgatt ttctccaact tggtaatcat caaggcgaac tgcctctcct gatcctgtgg
    14761 tatcaaatgg cgcatagtca tactcttcat aagctgcata gaccgcagcc tttgcctctt
    14821 cagtaggttc gactcgaacg ttgcgataac gagcaaggcc ggttcctgct gggatgagtt
    14881 ttccaatgat gacgttctct ttaaggccaa gaagtggatc actcttttca gaaagggctg
    14941 catcggttag gacgcgagtt gtctcctgga aggaagctgc tgatagccaa gattcagtag
    15001 caagtgatgc tttggtaatt cccataagtt ctgggcgacc atttgcagcc ttgccacctt
    15061 tctgcaccgc ttcgcggtta gcagattgga accttaggtg atcaactaat tcacctggca
    15121 atagatcagt atctcctggc tctagaacag tgatgcgacg aagcatctgc ttaaccacaa
    15181 tttcaatgtg cttatcgtga ataccaacac cctgggtgcg ataaacctct tgaatttcat
    15241 ggactagatg ggtctgggtt gatcttgctc ctaggatctt aagtacttgc ttaggatcaa
    15301 ttgcgcccac aaccatctgt tgaccaacgc ttaccttctg gccatcttca actagaagtt
    15361 tctgacgacg agtaattgga taagcaacag cctctgaacc atcttctggg gtaacaatta
    15421 tcttcttacc cttagcatct tcaaggaagc taacaactcc agcagtttct gcaattggag
    15481 caacgccctt tggagtgcgc gcttcaaaga gctcaactac gcgaggcaga ccatgggtga
    15541 tatcacctga tgcagttgcc gctcctccag tatggaaggt acgcattgtt aactgagttc
    15601 ctggttcacc aattgattgc gctgcaatga ttcccactgc ttcgcccaca tcaactagct
    15661 tgcctgatgc cattgagcgg ccataacact tagcgcactg tccaaccttg ctatcgcagg
    15721 taagaatcga gcgaacttta atctcgctaa cgccagcatc aattaactca ttgattaagc
    15781 gatcgccaag atcagcacct gatgtaacga tggtcttgcc atctactaca acatcatctg
    15841 caagggttcg gccatagatt gaagtctcaa cataatcatc gcgggttagt tttcctgcaa
    15901 cgatatttcc aatctttaga gttagaccgc gatcagttcc gcaatcctct tctctaatga
    15961 ttacatcttg agccacatcg cagaggcgtc tagttagata accagcatct gcggtgcgaa
    16021 gagcagtatc tgcaagtcct tttctagcac cgtgagttga gatgaagtac tcaagaactg
    16081 agagtccttc acggaagtta gatttaatcg gacgaggaat aatttcaccc tttggatttg
    16141 ctacaagtcc gcgcatacct gcaatctggc gaatctgcat catatttccg cgggctccag
    16201 agaagaccat catccagact ggatttgttc ttgggaaatt atcttccatc tccttagcaa
    16261 cttcatttgt tgcctgagtc cagatctcaa ttagctcctg acgacgttca tcatcggtaa
    16321 ttagaccctt ctcatattga gattggacct tatcggcctt tgtctcatag ccctcaagga
    16381 tttcaagctt tcttcctgga gttactacat caacaattcc aattgttgct ccagctctag
    16441 ttgcccaggt gaaaccaagt tccttcaagg catctagtac tgctgctaca actacctttg
    16501 gatagccttc agctaaattg tcaacgatta aaccaagtgc tttcttagtt acatcgtaat
    16561 caacatatgg gaatgactca ggaagaactt cattaaagat tgctcttcca agagttgtct
    16621 cttttgttac tagctcgcca ttgatattga cgcgaatctt tatcttggct tgtagtgaaa
    16681 ggctattttg ctcataggcc attagacctt cagagattga ggtaaagctg cgtccctcac
    16741 ccaattgatt ctcacgaagt gaggttagga aataaagacc tagcaccata tcttgggttg
    16801 gcgcagttag cggccttcca gatgctggcg aaagaatatt gtttgaggag agcatcaaga
    16861 tacgagcctc tgcttgagcc tcggcagata gtggaacgtg aaccgccatc tgatcaccat
    16921 cgaagtctgc gttaaaggca gtacaaacta gagggtgaat ctgaattgct tttccttcaa
    16981 ttagttgtgg ttcaaatgct tgaataccta aacggtgcag agttggtgcg cggtttagta
    17041 gcactggatg ctcagcaatg acctcttcta atacatccca aactacagat ctggaacgct
    17101 caaccatacg ctttgcgctc ttgatgttct gggcatgatt tagatctact aagcgcttca
    17161 ttacaaaggg cttaaatagc tcaagtgcca tctgcttagg cagaccgcat tgatgaagtt
    17221 ttagctgtgg accaacgacg attaccgaac gacctgagta atcaacgcgc tttccaagaa
    17281 ggttctgacg gaagcgtccc tgctttcctt ttaacatatc ggaaagtgat ttcagtggac
    17341 gatttccagg tcctgttact gggcgaccac gacgaccatt atcaaatagc gcatctactg
    17401 cttcttgaag catacgcttt tcgttattga caataatctc tggcgctcca agatctacta
    17461 atctcttcaa gcggttatta cggttgataa cgcggcgata gagatcattt agatcagagg
    17521 tagcaaagcg gccgccatct aattgaacca tcggacgaag atctggcgga ataactggaa
    17581 cgcaatctag aaccattgaa gctggcttgt tagaggtagt tagaaatgag ttaaccacct
    17641 taaggcgctt aatggcgcga gtcttcttct gtcccttgcc agtttcagct aaaacattta
    17701 gcttgtcgaa ctcagcttgg agatcaaagg tttcaagtct gcgctgaatt gcagcagctc
    17761 ccatatatcc cttgaagtag atgccatagc gatcacgcat ctcgcgatat agattttcat
    17821 caccttcaag atcttggacc ttaagattct taaaacgagt ccagacctcc tccaggcgat
    17881 caagttcttt ttgagaacgc tcacgaatat tttttagttc gcgctcgcca gcttctctta
    17941 ccttacggcg ttgatcagct ttggctccct catcttcgag ctctgctaaa tcattttcca
    18001 tcttctcttg gcgaagagca agatcgttat cgcgcttggt ttcaatcttc ttgcgatcag
    18061 agttcatctt cttttctagc tttggaagat cttcctcgcg cgcctctaca tcaacttcag
    18121 tgatcatgta ggcagcaaaa tagataacct tctcaagatc ttttggagca agatctagaa
    18181 gatagccaag acgtgatgga acgcccttga aataccagat atgagtaact ggggcagcaa
    18241 gttcaatatg tcccatgcgc tcacggcgaa ccttggcgcg agttacttca actccgcatc
    18301 gctcgcagat aattcctttg aagcgtacgc gcttatattt accgcagtag cactcccaat
    18361 ccctagttgg tccaaagatc ttctcatcaa agagaccatc tttttccggc tttagggtgc
    18421 ggtagttaat agtctctggc tttttgacct caccaaatga ccactcacgg atattgccag
    18481 cagaggcaag accaatcctt aactcatcga aaaagttgac atctaacata tctatttctc
    18541 cccctgtcaa acttcttcta cagaacttgg ttcaactcgt gacaagttga tacctagctc
    18601 ttcggctgtt ttaaagactt cttcatcagt atcgcgcatc tcaattgcaa caccttcaga
    18661 tgaaagtact tctacattca gacatagtga ttgcatctct tttactagaa ccttaaatga
    18721 ctctgggatt ccaggctctg ggatgttttc acccttaacg attgcttcat agaccttaac
    18781 gcgacctaga acatcatcag atttaattgt tagtaactct tgcaaggtgt aagcagcgcc
    18841 atatgcttca agggcccaaa cttccatctc accaaatcgc tgtccaccga actgagcttt
    18901 tccaccaagt ggttgctgag taatcatgga gtaaggacca gttgagcgag catggatctt
    18961 gtcatcaact aagtgatgta gcttcaagat atacatataa ccaacagtga ttggctcgcg
    19021 atatggctga ccagttctgc catcaaagag aatcgctttt ccttgttctc caactaaacg
    19081 atcgccatca gcatttggaa gagttgatcc aagaagtcct gtgatctcct cttctcttgc
    19141 gccatcaaag acaggtgttg ctacgcgagt tccaggctct gcagagagtg cgccaattgc
    19201 gcctaagcgc tcttgccatc ccttatcgga gccagatact tgccagccgc tctttgcaac
    19261 ccaaccaagg tgagtctcta gaacctgacc aacattcata cgtcctggaa caccgagtgg
    19321 attaagtact acatcaactg gagttccatc agcaagaaat ggcatatctt caactggcaa
    19381 aatcttggag ataacgccct tatttccatg gcgtcctgca agcttgtcac catcttgaat
    19441 cttgcgcttc tgtgcaacat agacgcgaac tacttgatta actccagctg caagatcgta
    19501 gccatcatca agatcaaata tttttacgcc aattactttt ccgccctcac catgaggaac
    19561 ctttagcgat gtatctctaa cttctcttgc cttctcacca aagattgcac gaagtaggcg
    19621 ctcttcagga gttaattcag tttcaccctt aggcgtaact tttccaacca agatatcgcc
    19681 tggaacaaca tctgctccga tgcggataat tccgcgctca tcaaggtctg caagaacttc
    19741 ctcagagaca tttggaatat cgcgagtgat ttcctctgct cctagtttgg tatcacgagc
    19801 atcaacttca tactcttcaa tgtgaattga ggagaggaca tcatcttgaa ctaaacgctg
    19861 ggacaaaata atggcatctt catagttatg accttcccat gacataaatg ccacaagtag
    19921 gttcttgccc aaagccatct cgccaagttc agtgcttgga ccatcagcaa tagcttggcc
    19981 ggcagcaatc ttctgtcctt ggctaacaat tactcgcata ttgcgagaag ttccttgatt
    20041 ggagcggatg aacttatcga cgttataagt atcttcctcg ccatcatcgg tcttaataac
    20101 gattagatct gaggcaacct cgcttaccac tccgcctcta acggcaagaa gaacatcgcc
    20161 agcatctact gctgcgcggt attccatacc ggttccaact agaggtgatt ctgcgcgaag
    20221 tagtggcaca gcctgacgca tcatgtttgc tcccataaga gcgcggttag catcatcatg
    20281 ctcaaggaat ggaatcatcg cagttgcaac cgaaaccatc tgtcgaggag agacatccat
    20341 gtaatcaact tcttcagctg ggatgtattc aacttctccg ccgcggcgac gaactaaaac
    20401 tcttgcttca gcaaagtggt tatcctcagt aagaggagca tttgcttgag caataatgtg
    20461 ttcatcttct tcatctgcag ttaagtaatc aaccgcatct gtaactttgc ctttagaaac
    20521 tttgcgatat ggcgtctcaa cgaaaccaaa tggcgtgatg cgagcataag ttgcaagaga
    20581 gccaatcaga ccaatgtttg gaccttctgg tgtttcaatc ggacacatgc ggccatagtg
    20641 agatggatga acatctctta cttcaaagcc agctctatct cttgaaagtc ctccaggtcc
    20701 aagcgctgat agacgacgct tatgagtaag acctgaaatt ggattggtct gatccataaa
    20761 ctgtgagagc tgagatgttc caaagaactc tttgatggat gcagtaattg gacggatatt
    20821 gataagtgtc tgcggagtaa tcgcttcaac atcttgtgtt gtcatacgtt cacgaacaac
    20881 gcgctccata cgagagagac caactcgaac ctggttttga attaactcgc caacagttct
    20941 aaggcggcga tttccaaagt ggtcgatgtc atcaatctca acgcgaacct gcttacctaa
    21001 atcaataaga gtctcgcctt tatgaagggc tgcgatatag cgaattgctc cgacgatgtc
    21061 gtcgatagca agaagagatt taccaagatc tagatctagg ccaagcttct tattaacctt
    21121 aaaacgccct acttttgcta ggtcatagcg cttagggttg aaatagaggt tttcaataag
    21181 ggtttgtgct gcctctttag ttggcggctc acctggacgc atcttgcgat agatatcaag
    21241 cagagcttca tcttgagttg aaacgttatc tttagcaaga gtttccttaa tggattcata
    21301 ctcaccaaac tcagcaagaa tctgatcatt gctccagccc agtgctttaa ggaagatagt
    21361 tactgattgc ttgcgctttc tatcaatacg aacagcaaca aagtctttct tatcaatttc
    21421 aaactctaac catgctccgc ggctaggaat gatctttgct gtgaagatat ctttatcaga
    21481 tgccttctca actgttcttt caaaataaac tccaggagat ctaaccagct gtgaaacaac
    21541 tacgcgctct gttccattga taacaaatgt tccgcgcgga gtcattagcg ggaagtcacc
    21601 catgaatact gtctgggatt taatttcacc agttacattg ttagtaaatt ctgcggtaac
    21661 aaatagtgga gcggcatagg taatgtcgcg ctctttgcac tctgcaattg tgtatttgga
    21721 aggttcaaaa cgatgatcac ggaaggagag tgacatcgag ccttggaaat cttcaattgg
    21781 tgaaatctct tcaaatattt cttcaagtcc agaggtctta ggaatttctc ctcgatctgg
    21841 tctttcattc ttaccaagtc ttgcgcgcca ggcatcattg ccaagtaacc aatcaaagct
    21901 ctcgatttgc agagctaata gatttggaac ttcttgtggt tcgcggatct tggcgaaaga
    21961 aatgcggtta ggagctgttg atttcgtttt cgcggccaag agttttcctt ccaaagttag
    22021 tcactacttc tttaagtttt ttagtaactc ttaaatctct tcgtgctcgc acagcaaacg
    22081 taaggcctca gccgctatat gcctgacctg tgatttttga gcgagggggc aggatagccc
    22141 ttgcgacacg ccgctgtcta attcagtggc ttatatcaag ggcggcggca aatagcacct
    22201 aactaaattt agtgctaaat ttacccctaa ttaatggagg gtagacagat ggagaaaatc
    22261 ttaagtaaga gagcagtagc aatatctgag ttcaagcgag cgcctaatga aattgttgcc
    22321 gaggctaatg gtgagcccct agctgttcta actaataaca aaccaagctt ttatgtcatc
    22381 tccccttcag cctatgaaga acttctcgag cagctctggg agcttgaaat cactccactt
    22441 cttgccaagc ggttatctga tctcaaatca ggtaagagca aatctattca agtcacgatt
    22501 gaagatctta ttaagtgaag tatcaactca gattcctatc tgaggcatgg gatgaatgga
    22561 aagagctaga taagtcaatc aagatccctc tggctagaaa actcgaaaaa cgtctagaag
    22621 atcccaagat tgcctcagct caattgcacg gaaaattgat tgggctctac aaaatcaagc
    22681 tctctgactc tggatatcgc ttggtctatc aagtcttgga taaagaagtg gtggttttag
    22741 ttatctcagt tggtaggcgt gataagtcga aggcttatga cttagctcaa gagagaatta
    22801 aaaaagcccc gccaagatga cggggccttc ttaatctcta aattaactta cttgatgctg
    22861 acctttgcgc cagctgcctc aagggcggcc tttgccttat cagctgttgc cttatcagcc
    22921 ttctcaagca cggttgctgg agctgaatcg acaagatcct ttgcttcctt tagaccgagg
    22981 cttgaattaa gggcgcgaac ttccttaatc accgcgatct tctgtgaacc ggcttcttca
    23041 attactactg tgaattcatc ttgagctgct gcagcttctc cgccgcctgc tgccccgcct
    23101 gctgctgcaa cagcaactgg cgctgcggca gtaacatcga attcagtctc aaatgccttt
    23161 acgaaatctg agagctcaac aagagtcatc tctttgaatt gagctaatag gtctgtgcta
    23221 cttagttttg ccattttcgt atttcctttt ctttaagcgt tctctgttgg ttctactggc
    23281 gcagaagtct ctggcgctgc ctcagcaaca acttcctcgt ttgttgcttc tgcaactggc
    23341 gctgcctcgg ctacaacttc ctgagttgtt gcctctgcaa ctggtgctgc ttctgcagcg
    23401 ggggcagcct gctctggcgc agcaggtgct ccagattcag cttctctctt aatacgcagc
    23461 gcatcaaatg ctctagcggc ttttgccata gatgccttca tagcgccagc aagcttggcc
    23521 aagagaactt cgcgcgattc aagatcggca agtttcatga tttcagctgt tgttactgct
    23581 tttccttcaa agattccacc cttaataatt aagaatggat tttccttctg gaaattcttt
    23641 aagctctttg cagctgcaac tgaatcgcct tgaacgaagg caacagcagt tggaccagtt
    23701 agtagtgagt cagcgacctc tactccagcc tcttttgcag caatctttac caaggtgttt
    23761 ttagcaactt tatatttagt gccaacacca agtgagcgac gtagctcttt aattgcagtc
    23821 acggttagac cgcgatactc ggtaacaaca gttgcatcgg cagacttaaa gtcttcaacc
    23881 aattcagcaa ctgatgccgc cttatcagag cgtgccatct agcttccttt ctatttatat
    23941 tccccagaag aagtacctac tgattgggtg ataaatataa aaaaaccccg tcgcatagaa
    24001 acgcgcgggg tatccgctcg aacctatgcg ggttgtcttt cgacatttac ttcaaacctt
    24061 gcgatttgaa accagcagtc tctggtgaga gggcagatat tagaagcaaa gggggcttta
    24121 ggtcaaaatc acgcctgtct gaacccatct tcaaagaaat cagggcgctt cactagggct
    24181 agataacaga agcgattctc cacattttcc tcaaattaga aaacacactt gctctaatta
    24241 ttttagattt caaaagcatg ccgttcgtcg tctaatggca ggatgcttgg ttttcaacct
    24301 tgaggtgtgg gttcgagtcc taccgaacgg gcatgtgtaa ccccgcaaag aatgcggggt
    24361 ttaccgaacg ggccagatcg agagaaagct cgatcaactt aatgctaatt atggcacaga
    24421 tttatctcta aaaacggttt gactacagag aagagatgta aaaattaaga tataaagatt
    24481 tgcgatgatt ttttcatcta ctcagagtaa agaagttgga tagagcagat ttacagaact
    24541 atctcttttt aaatcccttg ggacactttg gattgaccgc agttactttt ttaacactct
    24601 tacctttaac gcaggtaata gttgttttct tagctactgc tgccttagtt ggggttggtg
    24661 ccgcaattgt gggagttgga gatggagaag caacagctga ttctgtgggc gttggtgttg
    24721 gagttggagt tggagttgga gttggagttg gtgttggtgt tggtgttggt gttggagtca
    24781 ccactgcagc aggagtaaat ctcaatacac ctcgcttaac gataaatgtt tgtg
//
LOCUS       DAOROD010000016        24404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_66_length_24404_cov_26.422235, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000016 DAOROD010000000
VERSION     DAOROD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 24404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24404
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_24404_cov_26.422235"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLW53_04290"
     CDS             <1..180
                     /locus_tag="PLW53_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36674.1"
                     /translation="PERALRQVGVLMVGICSLLTLLTFLTPAPWGIGKVAYLLVGAFF
                     TLAYIYGLRGRRRNY"
     gene            289..804
                     /locus_tag="PLW53_04295"
     CDS             289..804
                     /locus_tag="PLW53_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD36675.1"
                     /translation="MEKNQVNSQELGYRGASACSAAGISYRQLDYWARTGLVEPTVRG
                     ASGSGSSRLYGFKDILVLKIVKRLLDTGVSLQNIRSAVTHLRERGVEDLARITLMSDG
                     ASIYECTSSEEIFDLLQGGQGVFGIAIGKVWNEVEGSLSHLQAENVSDGAISAPSDSD
                     ELAARRKLRGA"
     gene            833..3676
                     /gene="gcvP"
                     /locus_tag="PLW53_04300"
     CDS             833..3676
                     /gene="gcvP"
                     /locus_tag="PLW53_04300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRD36676.1"
                     /translation="MARAEFPDRHIGPSSDEISTMLLELGYSDLESFTQAVLPDSIKL
                     TSKFGPLLPKPISEVEVIAELRKLASENRKMESLIGLGYYASITPPVVLRNVLENPAW
                     YTAYTPYQPEISQGRLEALFTFQTMVSDLTGLPICNASMLDEPTAAAEAMTLARRSTK
                     LGDEARFVIDSRVHPQTQAVVRTRAKPLKINCEVLDLSSGVDLSNCFGVLLQYPDTFG
                     SLNDFSDAIAKAHNGGALVIMASDLLALTLIKSPGEWGADIAVGSAQRFGVPMGFGGP
                     HAGFLAVREGLERSLPGRLVGQSIDSHGNPALRLALQTREQHIRRDKATSNICTAQVL
                     LAVISAFYGAWHGPEGLKEIAVRTHQLARKLAGSLKESGFELYSPNFFDTVSFLASNS
                     DALIDAALKAGINVRKISGGVSVAFDEVSTNATVEKLIRAWGLKQNSSEPYGAIASLR
                     STSYMQHPVFNSYHSETAMLRYIRTLSDRDLALDRTMIPLGSCTMKLNATTEMEAITW
                     PEFADLHPFAPSNQSLGTRKIVSELSKWLIDITGYDAVSLQPNAGSQGEFAGLLAIRN
                     YLDSLGQSQRDICLIPSSAHGTNAASAVMAGMRVVVIACDEAGNVSLDDLKAKIAEYG
                     EQLAALMVTYPSTHGVFESEISQICALIHEAGGQVYVDGANLNALVGLAQPGKFGADV
                     SHLNLHKTFCIPHGGGGPGVGPVIAKKHLGPFLPNHPLDSSAGPLTGPGPISGAPFGS
                     ALILPISWAYIRMMGAEGLTKATQVAILSANYIAKKLAPDYPTLYTGKNDLIAHECII
                     DIREITKRSGVSVDDIAKRLMDYGFHAPTMSFPVPGTLMIEPTESEDITEIDRFIAAL
                     RSIAKEIRAIESGEISIEDSPLRNAPHTVEALVSDTWDRPYSRQDAALPMGSEIGMER
                     RGKYWPTVGRVDGAHGDRNLVCACEPIESLAIN"
     gene            complement(3698..5104)
                     /gene="lnt"
                     /locus_tag="PLW53_04305"
     CDS             complement(3698..5104)
                     /gene="lnt"
                     /locus_tag="PLW53_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRD36677.1"
                     /translation="MRKVTLALISGLLLFAAFPPIEFWPAAFLGAGLLFLLLAEERLL
                     ARFPIALLAGGAFFAPLLHWSSIYVGAIPWLILALGESLIFALIALAPIRKNAYGAIA
                     FASSFTLIELLRMKAPFGGFGWGRIGFTQVDSLQFLYPFIGVVGISFFAISLAAIIVV
                     KPISLLAFIPLIILSFFNFNINTSSEKKIEVIAVQGSVPQLGLEFNQRALAVLNNHID
                     ATPKQSSADLIIWPENASDVDPITNLKARQGIEKLLSEIQKPLLIGAVEQRDSGPLNS
                     SLLFGADGELLSRYLKQDLAPFGEYIPLRSVSEAITPLAKQVRDFIPGEAWVKHKVNG
                     IEFQSFICFEVLDDDHIQAGAVGAEFLVAQTNNATFGDSYQAAQQLQITRARAAELSR
                     QFAVVSTTGYTAHIDQNGEILDIAEQFKPIALEMEIETFKDRTTTIRGSLLWMGILGV
                     LWLLSVFGQRRSRSVFSR"
     gene            complement(5108..6013)
                     /locus_tag="PLW53_04310"
     CDS             complement(5108..6013)
                     /locus_tag="PLW53_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HRD36678.1"
                     /translation="MSLLLIDSASLWYRAYYGMPDTLLSPQGEPINAVKGFIDMSARL
                     INQYKPNRLALCLDGDWRPSWRVELFPGYKLNRVDDDGEGEEEPDLLTPQVPLILDFF
                     EAAGIAIVGMDDYEADDVIATFATREKGPIRIATGDRDLFQLVDEERDVKVAYLAKGI
                     SNHDLVNHSWIEKKYEIPGERYALFAMIRGDASDGLPGIRGIGEKGAAEIAKNYSSMA
                     DLIKAAKSEDPKLSPNHRKKLLADLDYAAVADRLVQCARDVNLPKIDLSIPRSAKNAK
                     YLETMKSDYGLGASVDRLLSALNWS"
     gene            complement(6010..8391)
                     /locus_tag="PLW53_04315"
     CDS             complement(6010..8391)
                     /locus_tag="PLW53_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD36679.1"
                     /translation="MSTPSPAERYAAAKKRSSHPLTTEFMANYDFGFDEFQSLGCHHL
                     EDGKSVLVAAPTGAGKTIVGEFATFLAEKNGNRCFYTTPIKALSNQKYQDLVKLYGDS
                     EVGLLTGDSSINSEARIVVMTTEVLRNMIYAGSDAIDDLGYVVMDEVHYLADKFRGAV
                     WEEILIHLPERIQIVSLSATVSNAEEFGDWLKTVRGETEVVLSEIRPVPLYQHILIGN
                     RILDLFVDEGRVNPEILRIEKNSIKRVPGGSKSWKESKFSSIKSLTRPEVVEKLSERG
                     FLPAIFFIFSRAGCDGAVASCIREGLSLTNSLEKEEIRRVIALKTHELPSEDFGVLGF
                     HEWAQALERGIAAHHAGLLPMFKETIEELFQRGLLKIVFATETLALGINMPARSVVLE
                     KLVKWNGESHVNVSPGEYTQLTGRAGRRGIDIEGNALILWNEQMDSSIAAGLASTRTY
                     PLRSSFSPTYNMSANLISRFGHERARKSLGFSFAQFQADKAVVGLARQIAKNEVAIKA
                     LIEGSQSDLGDMGEYMKARFAIKELEAQFSSRRITGGSRRKHHENIEEQISELRRFLK
                     HHPCHSCPNRESNARGFEKAARLEKESAGLKARMEGRTNVIPRTFDRVSEVLKELNYL
                     SGDQLTPKGGVLTKIYAESDLLLSELISSDLLNQFSPADLVGLLSALVYDGRGERSRS
                     PRLPKTLDASIPMMMKVWLNIVKLEEDHGITPQKEPNFDLAWSAYRWANGHSLQTILR
                     ETEITVGDFVRAIRQIIDLLGQLLNANPQMATTVKEAVKKIDRGVIAYSAVVA"
     gene            complement(8388..9257)
                     /locus_tag="PLW53_04320"
     CDS             complement(8388..9257)
                     /locus_tag="PLW53_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRD36680.1"
                     /translation="MWLLLINPKSGNGRGNKVGKLVKQEFKALGIQYLDISADSAQQS
                     QENLKRKLRENQKFEGLFLIGGDGTVNLAVQELVGSDLGLALIPAGTGNDFARTLNLK
                     LKNPEQLIKHYLSSRPSLIDVGKVGEKYFVDVLSTGFDSMVNERANAMKRIKGRAKYN
                     ISILLVLSTFKPKNYRFSIDGFSFESKAMLIAVSNGICYGGGMKVTPDAKIDDGLFDI
                     LILSPVSKLEFLKVFPKVFSGKHTSHPAVKISRGRSVEIDSDAVAYADGERVGPLPVK
                     ARVIERGLLTWRM"
     gene            complement(9272..10102)
                     /gene="tatC"
                     /locus_tag="PLW53_04325"
     CDS             complement(9272..10102)
                     /gene="tatC"
                     /locus_tag="PLW53_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRD36681.1"
                     /translation="MAARERGTMPLLDHLRELRKRIIRAAFFILIFSILGFVYYNQII
                     TTLAEPVCDLKLAQSSGSNNCGSLFISGVLGPLNLQVKVAFLTGIIVSAPFWLYQLWA
                     FIAPALHRKERRKSVLFIIAATPFFTLGAALAYYILPIAIRVLFGFTPESLNNLVRFD
                     DYLSFVLRIILIFGLAFELPVFLVSLNLIGVLSGKAILKPWRFAIFGITVFVAAFSPT
                     ADPLSMAALAVPLIVFYFGAGAIALLVDKKRDRKSKQIGDNQAADIDQASPIDEPLPE
                     "
     gene            complement(10178..10366)
                     /gene="tatA"
                     /locus_tag="PLW53_04330"
     CDS             complement(10178..10366)
                     /gene="tatA"
                     /locus_tag="PLW53_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRD36682.1"
                     /translation="MFGRNPQMWLLVILVVVILFGAKRLPDSARSLGRSLRIFKSEMK
                     ELNKDEKKSEEGDTSAKS"
     gene            complement(10451..11368)
                     /locus_tag="PLW53_04335"
     CDS             complement(10451..11368)
                     /locus_tag="PLW53_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRD36683.1"
                     /translation="MVENAALRAIRLLDLVPYIVAHPGISITELAKEFSITRDEVLKD
                     LNLLFLCGLPGYTPLELIDISFDEESVVIRDPQNLAAPRNLNESEALIARIALAALEE
                     STPRTSSAYLQIVALREKIAKAFSSSIPASAITFTLDKERATLEAIEAAIKQELDLEI
                     TYNNVTKDSSSRRSITPISIIAEDKRTLVSAYCHSAKALRTFNLAQISEVSTKARIVR
                     ADLERLEDSRGSSAEVIIKSEDSSFLSENASSLKELSKSCYQIDIFQPEWIVRSVLAG
                     ADSIELAKPLELRAEIAERANRALLAYKG"
     gene            complement(11361..12311)
                     /locus_tag="PLW53_04340"
     CDS             complement(11361..12311)
                     /locus_tag="PLW53_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRD36684.1"
                     /translation="MSSAKTERLVNLTMALLATSRYMQKSEIFRKVAGYSGTQETKER
                     MFERDKDDLRALGIEIEVASADPLFEDEPGYRIKPETYQMPIKSFTPTEIGILSTALG
                     LWLDSSLEDIALGAVRRVNSTALLDPALEINSLRTLNFSAEGLLDVTKALANRSEIAF
                     SYRKAGASKSDNRRVNPLGLSAWKGEWYLVGEDLDRSDIRTFNFSRISSKIDISKKRD
                     MYEIPSDFNIKDYLIMHNAEGIEIKAKVKKAQAEALRAKALEIKNLDEEWDEISYQSE
                     SSPAALQEALWYCDSLIIEQPEELRSLVVASLEKVAKSHG"
     gene            12357..12881
                     /locus_tag="PLW53_04345"
     CDS             12357..12881
                     /locus_tag="PLW53_04345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRD36685.1"
                     /translation="MKPSRIIVILASLTLLISLTACTTDGGAPSDGPKGQGNQSTGGN
                     LSNKYVEVSAEAGVAPTISSPSGTPPTTLVTEDVIIGSGKVAGANSTLTVHYTLMAWS
                     TGEIVESSWSGEPAQFPLSGVILGWQQGIPGMAVGGRRLLVIPPDLGYGAAGGGPIAP
                     NETLIFVVDLIDVA"
     gene            complement(12878..13738)
                     /locus_tag="PLW53_04350"
     CDS             complement(12878..13738)
                     /locus_tag="PLW53_04350"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HRD36686.1"
                     /translation="MQVIGSGPFKEGDRVQLTDSKGKMYSFYLSKGGQWHSHKGWINH
                     DSIIGVSEGTTLLSSSGTQYQAMRPLLNDYLLSMPRGATIIYPKDSAVIVGYADIFPG
                     ANVLEAGAGSGALSISLLRAIGQNGQLTSYEARDEFLENAKSNVKNYFGELPSNWKLL
                     HGRVEEAQFSGQFDRVIFDMLAPWDCLESAFQALIPGGVLCCYVATTTQLSKTAEALK
                     ARGDFSEPESFETLLRSWHHEGLAVRPMHSMNAHTGFLLFTRKLAEGNAPIRRKRRPS
                     KSASDLVDEA"
     gene            complement(13746..14438)
                     /locus_tag="PLW53_04355"
     CDS             complement(13746..14438)
                     /locus_tag="PLW53_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRD36687.1"
                     /translation="MLISDFPFKAVLWDMDGTLIDSEPIWIGEERRIMESLGATWSDE
                     DALYCIGGPMARVDAYMRSKLPVNKRDEFGEYALTKILLESMVERLKQKVPFAPGAKD
                     LIEQMNQARIPMALVTASNRAIMEGALASIGRHYFKTTISDNDVERSKPDPQGYQLAA
                     SRIGVPIEDCLIIEDSITGATAAIASGAYLLGVTHSGPLPMDEKVCHVENLIDLELSG
                     IVELFQPLLVKN"
     gene            complement(14428..17931)
                     /gene="metH"
                     /locus_tag="PLW53_04360"
     CDS             complement(14428..17931)
                     /gene="metH"
                     /locus_tag="PLW53_04360"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRD36688.1"
                     /translation="MSASKLKEASSLRLALGKRVVIADGAMGTMIQSANPSLDDFQGL
                     EGCNEVLNITRPELIADIHRQYFSAGVDAVETNTFGANLANLGEYDIPERIYELAEAG
                     ARIARGVADEFSISSGEPKWVLGSLGPGTKLPTLGHTTYEDLRAAYQEASRGLIDGGV
                     DALLIETTQDLLQAKAAVNGARAAVDNCDKDILIIAQVTVETTGTMLLGSEISAALSS
                     LQSLGVDLIGMNCATGPAEMSEHLRTLSKSSPVGISVMPNAGLPILKDGGAYYPLGPD
                     ELAISLDQFVKDYSVGLIGGCCGTTPEHLAAVVRKVHGTALKERAFTDEAGASSLYQF
                     VPFRQQNTYLAIGERTNANGSRAFRDALLAEDWQKCVDIAKDQVREGAHMLDLSVDYV
                     GRDGVKDMRELASRFATATTLPIVLDSTEPAVIKAGLESLGGRCVINSVNYEDGDGPD
                     SRFARIMPIAKEHGAALIALTIDEEGQARSAEWKLRVARRLIKDLNEKWGIKTSDIII
                     DCLTFPIATGQEETRKDGLETINAIAQLKKEFPQVQTTLGISNVSFGLNPAARIVLNS
                     VFLAEAVKAGLDSAIVHPSKISPISRIPEEQREVALDLIYDRRKFEGDDCVYDPLSKL
                     LELFAGVEVTSTRQSRAAELAALPIEQRLVRRIIDGEKAGLEADLDQARLGGIAPLAI
                     INDYLLEGMKEVGELFGRGDMQLPFVLQSAEVMKSAVALLEPHMEKSDASDRGSILLA
                     TVKGDVHDIGKNLVDIILTNNGYRVVNIGIKQTINQIIEAAEQSSVDVIGMSGLLVKS
                     TVIMKENLEELERRGLAEKWPVILGGAALTRTFVEKDLAEAFPGTVRYARDAFEGLSL
                     MDTMMGVKKGVEGVSLPPLKERRTRVTSIDAPLTTDTTRSDVASDNQVPKVPFFGSRV
                     VKGIALADYSSMLDERALYVGQWGLKSERGKYEEMVENQGRPRLRSLLNDAVSQGWIN
                     AAVVYGYFPCYSEGNDLVILHHEGEKKDSERLRFTFPRQRRDRRLCLSDFFKSKESGQ
                     IDVVAFHVVTMGQSVSQATAKLFEANEYREYLELHGLSVQLTEALAEHWHARIRSELG
                     FAGDDSSELSEILDQGYRGSRYSFGYPACPDLAAQVQLCELLEPKRIGVELSEEFQLH
                     PEQSTSAIIVHHPEAKYFNAN"
     gene            17960..18805
                     /locus_tag="PLW53_04365"
     CDS             17960..18805
                     /locus_tag="PLW53_04365"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HRD36689.1"
                     /translation="MHLSESSFNLRSPILIAAFEGWNDAAESATSAITHLLTIWSHQQ
                     IAVVDSEEYYDFQVNRPQIKIDESNIREIIWPGTIVYAVSSPALANDFLIVRGVEPSM
                     RWKSFISDLLDLADDYEVSMIISIGALLADTPHSRPFNVAISGAHPEVSERFDLEISK
                     YEGPTGIIGVLQDGAFRRGIDAISLWAAVPHYVATPPCPKAALALINALEDFLEISLP
                     QGDLPERAKLWEAQVDQMAADDAEVGDYVRQLESSKDSAELPEISGESIAREVERFLR
                     RNPGI"
     gene            complement(18802..20034)
                     /gene="mshC"
                     /locus_tag="PLW53_04370"
     CDS             complement(18802..20034)
                     /gene="mshC"
                     /locus_tag="PLW53_04370"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HRD36690.1"
                     /translation="MLSWPDVYMPPALAGINHPTLALFDSYKGKKVEITGEQNSIYVC
                     GITPYDATHMGHAATYLAFDLVNRYLKANGKKVSFVENITDIDDPLLERAARDSQDWK
                     ELAASQIDLFREDMTALSVLPPSAYRGVIESMAEIISLVEQMVASGKTYELEGDIYLD
                     LQQVDGAIQDLPLPLERALAIFKERGGDPERVGKRHGLDPLLWKARAGNDPYWIAPFG
                     EGRPGWHIECTAIAIKNLPETSPTSITLQAGGSDLIFPHHYMTALQAKALTGVEFASC
                     YSHAGMIGLDGEKMSKSKGNLVFVSKLRQEGVSPAVIRLALFADKYSSDRMWSKELLD
                     SAAELLDRLLSALSRQEVAPTFEVVQEVVNALADDLDTPRVFAALSNWCAKTEAGQVG
                     GSPGEISRALDTYLGITL"
     gene            complement(20074..20775)
                     /locus_tag="PLW53_04375"
     CDS             complement(20074..20775)
                     /locus_tag="PLW53_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HRD36691.1"
                     /translation="MLSREELLSGELIVEGRLVDASNATLFGKVLTSDQSSFSVVYKP
                     IAGERALWDFANGNLASREVASHLISEVGQFNCVPLTVMRDGPFGMGAVQQWIDLDPN
                     LDLIAIGQQSTPAIRNLALFDVVINNTDRKFGHILPISESEILGCDHGLTFHEEFKLR
                     TVLWQYAGQELDKEEISKLESLKRRISGDIGASLGELITKDEIESLLERIEMLISEGF
                     PYPSEQWPAVPWPPV"
     gene            complement(20776..21309)
                     /locus_tag="PLW53_04380"
     CDS             complement(20776..21309)
                     /locus_tag="PLW53_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HRD36692.1"
                     /translation="MSLRRIYNLDEVDRFILGTVGQPGEREFYIQVKKAGQVFSFALE
                     KAQAQALTDRFKEMLREVKSSEGAAPRDNEALDMPIDSEFTLGVMALTWQIDSQMIRF
                     EGQAITGDQGEEVIFDELASDETEGAPAILRIRITPSQARSFIDRASSVIKAGRPPCM
                     FCGAPINPDGHICPRMN"
     gene            complement(21299..21964)
                     /locus_tag="PLW53_04385"
     CDS             complement(21299..21964)
                     /locus_tag="PLW53_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD36693.1"
                     /translation="MTTFLLLRHAHSVANNAGILAGQLEGIGLSKDGIAQVKKLNSSF
                     ENLKIDRIISSPMQRCLETIEGIAKSKKKRISIDKRLIEMNYGSWSGKKLSKLSRMKD
                     WKVIQTKPSAFRFPQGESFKELEKRIESLLKELTRKYPKETVLLITHGDVIKIAASLT
                     VGSGLDNFQKFAVDPCSLTTLSWGPKSRIMMTFNQKIVSIKSSKKKKRRAGNILGGSK
                     DVA"
     gene            22029..22976
                     /locus_tag="PLW53_04390"
     CDS             22029..22976
                     /locus_tag="PLW53_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD36694.1"
                     /translation="MELRQLGRSGVRVSRLGLGTMTWGRDTDEHEAAEQLRFYLDAGG
                     NFIDTAAVYGNGDAERVLGGFLGVMVPRDQIIIATKAGISFKSGERVVDNSRTSLIAD
                     LDNSLSHLRTEYVDLWQIHTWDEKTPLEEVLSALDYAVSTGRARHVGISNFAGWQLAR
                     AVTLQNPIFGKAPLISTQNEYSLLNRKIEREILPASRELGVGVLAWSPLGRGVLTGKY
                     RSGLPSDSRGASPHFSSFIEPYLDERSRKIVEAVMVAADGLGLSPLEVSLAWVRDCPG
                     VTSAIIGARTGAQLRGALSAESVTLPVPVREALDEISAI"
     gene            complement(22973..24292)
                     /locus_tag="PLW53_04395"
     CDS             complement(22973..24292)
                     /locus_tag="PLW53_04395"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD36695.1"
                     /translation="MKLTDAQRKELEEDVVKLCQELIQIPSVNFGEGKGDEKAAAEYV
                     AAKMKEVGIQSKIYESAPNRCSVVARIEGKDKSKPGLVVNGHLDVVPANAADWSVDPF
                     SGAIKDGCIWGRGAVDMKNMDAMILAVIRLWARYNYQPERTIVIVFFGDEEAGGIYGS
                     RWMAEKHPEVFAGCSETISEVGGFSLTLLSGKRVYAIEASQKGIEWMKLTAEGVAGHG
                     SMVNNANAVTRLTEAIAKIGNYTWPQRITKTSDLFFQKISELSGKPYDRNNLQPLIDE
                     VGPMGKMIGATLCNTTNPTMLEAGYKANVIPQSASAVVDGRTLPGYEKELLDTVKSLV
                     GEHVKVESLVSDIPLEVEFGGPLVDAMIAAIKSEDPDGIPIPYLLSGGTDNKALAKLG
                     IVGYGFSPLKLPPDLDFTGLFHGIDERVPIDSLQFGARTLFHFLVNA"
     gene            24314..24397
                     /locus_tag="PLW53_04400"
     tRNA            24314..24397
                     /locus_tag="PLW53_04400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24348..24350,aa:Leu,seq:gag)
BASE COUNT         6607 a         5679 c         5245 g         6873 t
ORIGIN      
        1 ccggagcgag ctctacgaca agtgggagtt ttgatggttg ggatctgtag ccttttaacg
       61 ctccttactt tcttaactcc cgcaccttgg gggataggca aagtggccta ccttctagtg
      121 ggagccttct ttactctggc ctatatctat gggttgagag ggcgaagacg aaactattaa
      181 cctcaacttt aggtttggag agtgtcggtc gttgattcca agcatcgcaa gccttaccgt
      241 tacttcctta cccgcagaac ttccctgatt gaattgcgag gcgcataaat ggagaagaat
      301 caagtgaact ctcaagaact tggttatcgc ggcgcctctg cctgctcagc agccggaatc
      361 tcctatagac agctcgatta ctgggcaaga actggtttgg ttgaaccaac tgttagggga
      421 gcaagcggtt ctggaagttc aaggctctat ggttttaaag atatcttggt tctaaaaatt
      481 gttaagcgcc ttctcgatac tggcgtatcg ctacaaaaca ttagaagcgc tgttacccat
      541 cttcgcgagc gcggagtgga ggatctagcc agaatcacgc taatgagcga tggtgcttca
      601 atctatgagt gcactagctc tgaggagatc tttgacctgc tgcagggagg ccaaggggta
      661 tttggtatcg ctattggcaa agtctggaat gaggttgaag gctcgctctc tcaccttcaa
      721 gcagagaatg taagcgatgg agcgatatct gccccaagtg attcagatga acttgctgca
      781 agacgcaaac ttagaggggc gtaagtaaag cccagcccct atcctttccg ccatggcgcg
      841 agcggaattt ccagatcggc atataggtcc atcaagtgat gagatctcaa caatgctcct
      901 tgagcttggc tatagcgatt tagaatcttt tacccaggct gttcttcctg attcaatcaa
      961 attaacttct aagtttggcc cgctacttcc aaaaccaatt tcagaggtcg aagtaattgc
     1021 ggagttaaga aagttagctt cagaaaatcg caaaatggaa tcactaattg gacttggtta
     1081 ctacgcaagt attactccgc cggtagtgct tagaaatgtt ttggaaaatc cagcttggta
     1141 taccgcatac accccttacc aaccagagat tagccaggga agacttgaag ccctctttac
     1201 cttccaaact atggttagcg atctaacggg cttgccaatc tgtaatgcct caatgcttga
     1261 tgaaccaaca gctgcagcag aggcaatgac actggcaagg cgaagcacca agcttggcga
     1321 tgaagcgcga tttgtaattg attcaagggt tcatccgcag actcaagcag tggtgaggac
     1381 aagagctaag ccgctaaaga ttaattgcga agttcttgat ttaagtagtg gcgttgattt
     1441 gagtaattgc tttggagtcc ttctccaata tccagatact tttggatctc taaatgattt
     1501 ctctgatgca attgcaaaag cgcataacgg cggcgcgtta gtaattatgg caagtgatct
     1561 tcttgcattg actttgatta aatctccggg ggagtgggga gcagatatcg cagttggctc
     1621 tgcccagaga tttggcgttc ctatgggatt tggtggaccg catgcaggtt ttcttgcagt
     1681 aagagagggt cttgagagat cacttccagg aaggcttgtt ggacagagta ttgatagcca
     1741 tggaaatcca gctctgcgtc tagctcttca gacaagagag caacatataa ggcgagataa
     1801 ggcaacaagt aatatctgca ctgctcaagt tcttctagcc gtcatctctg ccttttatgg
     1861 agcatggcat ggcccagaag gcttgaaaga aattgctgtt agaacacatc aacttgctag
     1921 aaaacttgcc ggctctctta aggagagcgg ctttgaactt tatagcccta acttctttga
     1981 cactgtttct tttcttgcaa gtaatagcga tgccttaatt gatgccgcgc tcaaagctgg
     2041 aatcaatgtg agaaagatca gcgggggagt aagcgttgcc tttgatgagg tgagcactaa
     2101 cgcaactgtt gagaaattga ttcgcgcctg gggtttaaag cagaatagct cggagccata
     2161 tggcgccatt gcatcgcttc gctcaacttc ctatatgcaa catccagtct ttaattctta
     2221 tcattcagag acagcgatgc ttcgctacat caggactctc tctgatagag atttagcact
     2281 tgatagaacg atgattcctc ttggttcttg cacgatgaaa ttaaatgcca caaccgagat
     2341 ggaagctatt acttggcctg aatttgcaga tcttcatccc tttgcgccaa gtaatcaatc
     2401 cctcggaaca agaaaaatcg tctctgagct ttctaagtgg ctcattgata tcactggata
     2461 tgacgcggta tcactccagc caaatgccgg ctctcaagga gagtttgcag gccttcttgc
     2521 gattagaaat tatctggatt cactgggtca gagtcagcgc gatatttgtt taattccatc
     2581 aagcgcgcat ggaacaaatg cggctagcgc cgttatggca ggaatgagag ttgttgtaat
     2641 cgcctgtgat gaggctggca atgtttcact tgatgatcta aaggcaaaaa tcgctgaata
     2701 tggcgagcaa ttagcggctt taatggttac ttatccatcg acccatggag tttttgaatc
     2761 agagattagc cagatctgcg ctcttatcca tgaagcaggt gggcaggttt atgttgatgg
     2821 cgctaatttg aatgctctgg ttggacttgc tcaacctgga aaatttggtg ctgatgtttc
     2881 acatctaaat cttcataaaa cattctgtat tccacatggc gggggaggac ctggtgttgg
     2941 tccggtaatt gctaagaaac atcttggacc tttcttgcca aatcatccac ttgattcatc
     3001 agcaggaccg ctaactggtc cagggccaat aagcggcgct ccttttggat cagcgttaat
     3061 tctgcctatc tcttgggcgt atataagaat gatgggggca gaaggtttaa caaaagcaac
     3121 tcaggtagcg attctttctg caaattacat cgcaaagaaa ctagctcctg attacccaac
     3181 tttatacact ggcaaaaatg acttaattgc tcatgaatgc attattgaca tcagagaaat
     3241 taccaagcga agtggtgtaa gcgttgacga tattgctaag cgtttgatgg attatggatt
     3301 ccacgcacca acgatgagct ttccagtacc tggcacgcta atgattgaac caacagaatc
     3361 tgaagatata accgagatag atcgctttat tgccgctcta aggtctattg ccaaggagat
     3421 tagggcaatt gaatcggggg agatttcaat tgaagattcc ccactacgaa acgccccgca
     3481 taccgttgaa gctttggtaa gtgatacttg ggataggcca tatagccgtc aggatgccgc
     3541 gctacccatg ggcagcgaga ttggaatgga gaggcgagga aagtattggc caacggttgg
     3601 aagggttgat ggagcccacg gggataggaa tttagtttgt gcctgcgagc cgattgagag
     3661 ccttgcaata aactagtttt actttacata atgtagatta tcggctaaag actgagcgtg
     3721 aacgcctctg tccaaataca ctcaaaagcc ataaaacacc caaaataccc atccagagaa
     3781 gacttcccct aatcgttgtt gttcgatctt taaaggtttc tatctccatt tccaaagcga
     3841 tcggcttgaa ttgttcggcg atatccaaaa tttcgccatt ttgatcaata tgagcggtgt
     3901 atccggtggt gctaacgact gcgaattgcc gtgataactc tgcagctctt gctctagtta
     3961 tttgaagttg ctgggctgct tgataactat cgccaaaggt ggcgttattg gtctgagcaa
     4021 caagaaattc tgccccaact gcgccagctt gaatgtgatc atcatctaga acttcaaagc
     4081 agataaagga ttggaactct atgccgttta ctttgtgctt tacccaagct tcccctggaa
     4141 tgaaatcgcg aacctgcttt gcaaggggtg taatcgcctc agatactgat ctaagcggaa
     4201 tatattctcc aaaaggagct aaatcttgct ttaagtatcg cgagagcaat tctccatcag
     4261 cgccaaataa aagactggag ttaagaggcc cagagtctct ttgttctaca gcgccaatta
     4321 gcaggggctt ttgtatctca cttagcaact tttctattcc ctgacgagct ttcaaattgg
     4381 ttatcgggtc gacatctgat gcgttctcag gccagatgat tagatcggcg ctactttgtt
     4441 tcggtgttgc atctatgtga ttattcagaa ctgcaagcgc tctttgatta aattccaaac
     4501 ctagttgtgg caccgatcct tgcacagcga taacttcaat tttcttttct gatgatgtat
     4561 taatattgaa attgaaaaaa ctcaaaataa ttagaggaat aaaggcaagc aaagatatcg
     4621 gtttaacaac aataattgcc gcaagtgata tcgcaaagaa gctgattcct actacgccga
     4681 taaaaggata gagaaattgc agggaatcaa cttgggtaaa tccgatacga ccccatccaa
     4741 accctccaaa tggcgctttc attctcaata actcaattaa ggtgaaagaa gaggcgaaag
     4801 cgattgcgcc ataagcattt tttcggatcg gcgctagagc aataagggca aaaatcaggc
     4861 tctcccccag ggccaatatc aaccagggta tagctcctac atagatagag ctccaatgca
     4921 gaagtggtgc aaagaaggct cccccagcta gaagcgcgat tggaaatctt gcgagtagcc
     4981 tctcctcagc cagtagaaga aaaagtaatc cagctccaag aaatgctgct ggccaaaatt
     5041 cgatgggtgg aaaagcggca aaaagtagta agccagaaat tagggcaagg gtgacttttc
     5101 tcattcttta tgaccaatta agagcagata gaagtcgatc aacgcttgct ccaagtccat
     5161 aatcgctctt catagtttca aggtatttag cattctttgc agatcttgga atactcaaat
     5221 caatcttggg aaggttcaca tctcttgcgc attgcacaag acgatctgca acagcggcgt
     5281 aatcaaggtc tgcgagtaat ttcttgcgat gatttggtga gagtttagga tcttctgatt
     5341 tagcagcctt gattagatca gccatgctgg aatagttctt tgcaatttca gcagctccct
     5401 tttcaccgat gcctctaata cctggcagtc cgtcagatgc atctcccctg atcattgcaa
     5461 agagagcgta tctctcacct ggtatttcat acttcttctc gatccatgag tgatttacca
     5521 gatcatgatt actgattcct tttgcaaggt aggccacctt tacatcgcgc tcttcatcaa
     5581 ctaattgaaa taagtctcga tcgccagttg caatgcgaat cggacccttc tccctggttg
     5641 caaatgttgc gattacatca tctgcttcat aatcatccat tccaacgatt gctatccctg
     5701 cagcttcaaa gaaatcaagg attagaggta cttgcggagt taagagatct ggctcttctt
     5761 caccttcgcc atcatcatct acgcgattga gtttgtatcc gggaaagagc tccactcgcc
     5821 aagacggtct ccaatcgcca tctaagcaga gggccaaacg atttggcttg tactgattaa
     5881 tcagccgtgc gctcatatcg ataaaacctt ttactgcatt tattggttcg ccttgaggcg
     5941 agagtaaggt atctggcatt ccgtaatagg cgcgatacca gagcgatgcg ctatcaatta
     6001 aaagtaggct catgccacca ccgcggagta ggcgataact cccctatcga tttttttcac
     6061 tgcttcctta actgttgtag ccatctgtgg attggcattt agtaattggc caagaagatc
     6121 aattatctga cggattgctc taacaaaatc tcccacggtt atttcagtct ctctcaaaat
     6181 agtttgaaga ctgtggccat tagcccaacg atacgcgctc catgccaggt caaaattcgg
     6241 ttccttctgt ggcgtaattc catgatcctc ttccagcttt acgatattta gccaaacttt
     6301 catcatcatt ggaattgaag catcaagagt ctttggaagt cttggactcc tactgcgctc
     6361 gcctcgcccg tcataaacca acgctgatag caacccgaca agatctgcag gagagaattg
     6421 gttaaggaga tctgagctaa ttagctcgga taaaagcaga tcactttctg catatatctt
     6481 ggtaagtact cctcccttag gagttaattg atcaccgctt aaataattca attcctttag
     6541 tacctcactt acgcgatcaa aagttcgagg aataacgttg gttctgccct ccatgcgcgc
     6601 tttaagtccc gctgattctt tttcaagcct tgcggctttc tcaaaaccac gggcattact
     6661 ctcgcgattc ggacagctat gacatggatg gtgtttgaga aatcttcgaa gctcgcttat
     6721 ctgctcttcg atattttcat ggtgttttct ccggctacca ccagtaatcc ttctagatga
     6781 gaattgagcc tcgagctcct taattgcaaa gcgcgccttc atatactcac ccatatcgcc
     6841 aagatcgctc tgagatcctt caattagagc tttgattgca acttcatttt tcgcaatctg
     6901 tctagccaaa ccgacaacag ctttatctgc ttgaaactgg gcgaaggaaa aaccaagcga
     6961 cttgcgtgcc cgttcatggc cgaatcttga aatcaaatta gcgctcatgt tataggttgg
     7021 agaaaaagat gaacgaagcg gataggttct tgtgcttgca agtcctgctg caatagagct
     7081 atccatctgt tcattccaaa gaattaatgc attgccttca atatctattc ctcgcctgcc
     7141 agctcttcca gttagttggg tgtactcccc tggtgaaaca ttgacatggc tctcgccatt
     7201 ccatttaaca agtttttcca aaaccacact tcttgctggc atattgattc caagagccaa
     7261 agtctcagtt gcaaaaacaa tctttagaag acctcgctga aagagctcct caatagtttc
     7321 tttaaacatt ggaagtagcc ctgcgtgatg tgcagcaata cctcgctcca gcgcttgggc
     7381 ccactcatga aaaccaagaa ctccaaagtc ttcagacggt agttcatgag tttttaaggc
     7441 aatgactctt cttatctcct ctttctcaag tgaattggtt agcgatagcc cctcacgaat
     7501 acaagatgca acagcgccat cgcatccggc ccgagagaag atgaaaaaga tcgctggaag
     7561 aaaaccgcgc tctgaaagct tttcaaccac ttctggtcta gtaagagatt taatagagct
     7621 aaatttgctc tctttccaac tcttgctccc accaggtacc ctctttatcg agttcttctc
     7681 aatccgcagg atttcgggat tcacccgacc ctcatcgaca aagagatcta gaattcgatt
     7741 tccgatcaag atgtgctgat agagcggaac gggccggatt tcactaagca ctacttctgt
     7801 ttcgccgcgt acggtcttta gccaatctcc aaattcctcg gcatttgaga cagtagctga
     7861 tagtgaaact atttgaattc gctctgggag atgaatcagg atctcttccc agaccgcccc
     7921 tcgaaactta tctgccagat aatgaacttc atccataacg acatagccga gatcatcaat
     7981 tgcatctgat ccggcataga tcatatttcg caaaacttcc gtggtcataa caacgatgcg
     8041 agcctcacta ttaatggatg aatctccagt aagaaggcca acttcagaat ctccataaag
     8101 ttttaccaag tcctgatatt tttgatttga tagcgcttta attggcgtgg tgtagaagca
     8161 acgattgcca ttcttttcag ccagaaacgt tgcaaactct ccaacaattg tctttccagc
     8221 tcctgtgggc gctgcaacta atacgctctt gccatcctct agatgatggc agcctaggct
     8281 ttgaaactca tcaaaaccaa agtcatagtt ggccataaat tcagttgtga gagggtgtga
     8341 acttcgtttc tttgccgcgg catatctttc agccggagat ggagtactca catgcgccac
     8401 gttagtaggc cgcgctctat tacgcgcgct ttaactggaa gtggcccaac tcgctctcca
     8461 tcggcatagg ccacggcatc agaatcaatt tcaacgcttc gaccccgcga aattttcact
     8521 gcggggtgtg aggtgtgctt tccggagaaa acctttggaa aaacttttag gaattctaat
     8581 ttagaaactg gactgagaat tagaatgtca aataatccgt catcgatttt tgcatcaggg
     8641 gtgaccttca tccctccccc ataacagatt ccattagaaa cagctatcag catggcttta
     8701 ctctcgaaag agaatccatc gatgctgaat ctatagtttt taggtttaaa ggttgataaa
     8761 accagaagta ttgaaatatt gtatttagct cttcctttaa ttctcttcat agcattagct
     8821 cgctcattaa ccattgaatc aaagcctgtg ctaaggacat ctacgaaata tttttctcct
     8881 acttttccaa catcgattaa tgaaggtcgg ctcgagaggt aatgcttgat taattgttct
     8941 gggttcttta gttttagatt gagcgtcctg gcgaaatcat ttccagttcc agcaggtatt
     9001 aaagctaaac caagatcact tcctactaac tcctgtactg cgaggttaac ggttccatcg
     9061 ccaccaatta ggaataggcc ttcgaatttc tggttttctc ttaatttcct ttttagattc
     9121 tcctgagact gttgagcgct atcggctgag atatctaaat attggatacc aagagctttg
     9181 aattcttgtt ttacaagctt tccgactttg tttccgcgtc catttcctga cttgggattt
     9241 atcagcagaa gccacattag aaaagatcaa tctattcagg aagaggttca tcaattggag
     9301 atgcttgatc aatgtctgct gcttgattat caccaatttg cttagatttt ctatctcgct
     9361 tcttatcgac taggagcgcg attgcgccag cgccaaaata aaacacgatg agcggcacag
     9421 caagagcggc catggagagc ggatctgcag ttggcgagaa agcagcgaca aaaacggtga
     9481 tgccaaagat tgcaaagcgc caaggcttta gtatcgcttt tccagatagg accccaatta
     9541 aattgagcga gactagaaaa accgggagct caaaagctag cccaaagatc aaaatgattc
     9601 gaagcacgaa actcaagtaa tcatcaaatc gaactaggtt atttaggctt tctggggtga
     9661 aaccaaagag aactctgata gcaataggaa ggatgtaata ggcaagagca gcgccaagtg
     9721 taaagaaggg cgtagctgca atgatgaaaa gaacagattt gcgtctctct ttcctatgta
     9781 gagcaggggc gataaaggcc cagagctgat agagccagaa aggcgcagag acaatgatgc
     9841 cagttaagaa tgcaactttg acttgaagat ttagtggacc taatacgccg ctaataaaaa
     9901 gagaaccgca gttgtttgaa ccactacttt gagccagctt caagtcacat actggttcag
     9961 ccaaggtagt aatgatctgg ttgtaataga caaagccgag aatggaaaat attaaaatga
    10021 aaaatgcggc ccggatgatt ctcttgcgta gttcacgcaa atgatccaat agaggcatcg
    10081 tgccgcgttc tctggctgcc atatcgctcc gtttacctaa agagtattaa tctctcccct
    10141 actggaagta ggcacctatt gaaatcttga tttcaactta ggatttagct gaagtatcgc
    10201 cttcttcgct cttcttctca tccttattca attccttcat ctcactcttg aaaattctaa
    10261 gtgagcgacc aagagagcgt gctgaatctg gtagacgttt tgcgccaaag agtatgacaa
    10321 cgaccagaat cactaagagc cacatttgtg gattacgacc gaacatgaga tcaacctttc
    10381 cagcaatcag gcccactttt aagtaaggct tagagccata cgctaccacg cgcaaaatca
    10441 gcctgtcttg ctacccctta taggccagaa gcgctctatt agcccgctct gcaatctctg
    10501 ctctaagttc aagaggttta gccagttcaa ttgaatcagc gccagctaga acggatcgaa
    10561 caatccactc tggctgaaag atatcgattt gataacaaga ctttgaaagc tccttaagcg
    10621 atgatgcatt ttcgctcaag aaactggaat cttcgctttt gataatgacc tcagcacttg
    10681 acccgcgaga atcctcaagc cgctctaaat ctgcgcgaac tattctcgct ttcgtagaaa
    10741 cttctgagat ttgagcaagg ttgaatgttc taagtgcttt tgcgctatgg cagtaagcac
    10801 tcactagcgt tctcttatct tctgcgataa tcgaaatcgg ggtgatgctt cttctcgatg
    10861 aagaatcttt tgtcacgttg ttataggtga tttctaaatc taattcttgc tttatcgcgg
    10921 cttcaattgc ctctagcgtt gctcgctctt tatcaagtgt gaaggtaata gctgatgctg
    10981 ggatagaact actaaaagcc tttgcgatct tctctcgcag ggcaacaatt tgtagatagg
    11041 ctgatgaagt tcgtggcgta ctttcttcca aagcagctag agcaattctt gcaatcaaag
    11101 cctcagactc attcaagttt cttggagctg caaggttttg aggatctctt atcaccacgc
    11161 tctcttcatc aaatgagata tcaatgagtt ccaatggcgt atatcctgga agtccgcata
    11221 gaaagagaag gtttaaatcc tttaacacct catcgcgggt gattgagaat tctttggcca
    11281 gctcagtaat ggaaattcct ggatgagcaa caatgtatgg gacaaggtct agtaagcgaa
    11341 tcgcgcgaag tgcagcattt tcaaccatga gatttcgcta ccttctcaag tgaagcaaca
    11401 actaaagacc ttaattcctc tggttgctca ataatcaaac tatcgcaata ccagagcgcc
    11461 tcttggaggg cggcaggact tgactcgctt tgataggaga tctcatccca ctcttcatca
    11521 aggtttttga tctcaagggc ctttgctcga agggcttccg cttgggcttt tttaaccttc
    11581 gctttaatct cgatgccttc ggcgttatgc ataatcaaat aatcttttat gttgaaatca
    11641 gagggtattt catacatatc gcgcttcttt gagatatcta tctttgagct gattcgtgaa
    11701 aagttaaatg tgcggatatc gcttcgatct aaatcttcgc caactaaata ccactcccct
    11761 ttccaagcac tcaatcccaa gggattaaca cggcgattat ctgatttaga agcccctgct
    11821 ttgcgatagg agaaggcaat ctctgatcta ttagcaagag cttttgttac atcaagtagc
    11881 ccttcagctg aaaaatttaa tgtgcgaaga gaattgattt caagtgctgg atcaagaagg
    11941 gcagttgaat taactcttct tacagcgcca agtgcaatat cttccaaact gctatctagc
    12001 cagagtccaa gagcggttga gagaattcca atttcggttg gagtaaatga tttgataggc
    12061 atttgatatg tctcaggttt tattcgataa cccggctcat cttcaaatag cgggtctgca
    12121 gaggcaactt caatctcaat tccaagcgct ctcaaatcat ccttatcacg ttcaaacatt
    12181 cgctctttag tttcttgagt tccgctatag ccagcaactt ttcgaaagat ctcacttttt
    12241 tgcatatagc gcgatgtggc aagcaaggcc atcgtaagat tgactaaacg ctcggtttta
    12301 gcactcgaca tggagatgag attagcgcgg cgggcgtgaa ttggtaggct taaaccatga
    12361 aaccaagccg aataatcgta attctcgcaa gtcttaccct tctaatttct ctaactgcct
    12421 gcaccacaga tggcggggct ccatcggatg gaccaaaggg tcaaggcaat caatcaacag
    12481 gaggaaactt gtctaataaa tatgtagagg taagtgctga agctggagta gctcccacaa
    12541 tctcatcacc cagcggaact cctccaacaa ctcttgttac agaagatgta attatcggct
    12601 caggtaaagt tgccggcgca aattcaactc ttactgttca ttacaccctt atggcttggt
    12661 caacgggcga aattgtcgaa tcttcctgga gcggggagcc tgcgcaattt ccattaagtg
    12721 gagtaattct tggttggcaa caaggaattc ctggaatggc agttggcgga agacgtcttc
    12781 tagttattcc gccagatcta ggttatggag cagctggcgg cggaccaatt gcgccaaatg
    12841 aaacgctgat tttcgttgtt gacttaattg atgttgctta agcctcatca actaaatcgc
    12901 tagctgattt actaggccta cgctttcttc taattggcgc attcccctct gccaattttc
    12961 tagtaaagag tagaaagccg gtgtgcgcat tcatcgagtg cattggcctg acagccaagc
    13021 cttcatgatg ccaagaccta agaagagttt caaaactctc tggctctgaa aaatcaccgc
    13081 gggcctttag cgcctcggct gtctttgaaa gctgagtagt tgttgccaca tagcaacaaa
    13141 gcactcctcc tggaattagc gcttgaaacg cgctctctaa acaatcccaa ggagcaagca
    13201 tgtcaaagat aactcgatcg aattgtcctg aaaattgcgc ttcttcaacc ctgccgtgaa
    13261 gcagtttcca attactaggt agctctccaa agtaattctt aacattgctc ttagcatttt
    13321 ctagaaattc atctcgggcc tcataggaag ttaattgccc gttctggcca atagctctaa
    13381 gaagtgagat tgataaagcg ccgcttccgg cgccagcttc caaaacgttt gctcccggaa
    13441 aaatatcggc atatccaacg atgacagcag aatcttttgg atagataatc gttgcacctc
    13501 ttggcattga gagcagatag tcattgagaa gcgggcgcat agcctgatac tgagttccac
    13561 tactactaag aagagtagtt ccctcgctta ctccgatgat ggagtcatga ttaatccaac
    13621 ctttatgaga gtgccattga ccaccttttg aaagatagaa ggagtacatc tttcccttgc
    13681 tatcggtcaa ttgaacccgg tcgccctctt tgaacggtcc tgaacctata acttgcattt
    13741 actcatcaat tctttactag tagtggttga aagagttcaa ctatccctga tagctctaaa
    13801 tcaatcaagt tttcaacgtg gcaaactttc tcatccatcg gaagtggtcc ggagtgagtg
    13861 acgcctaaca gataagctcc cgatgcaatt gcagccgtcg ctccagttat tgaatcctcg
    13921 attatcaaac aatcttcgat aggcacaccg attcttgaag cagctaattg ataaccctgt
    13981 ggatctggtt tagatctttc aacatcatta tctgaaatcg tagttttgaa atagtgcctt
    14041 ccaatacttg ccaaagcacc ttccataatc gctctattag atgctgtaac aagtgccatt
    14101 ggaatccttg cctgattcat ctgctcaatc aaatctttag cacctggcgc gaaaggaact
    14161 ttttgcttta atctctctac cattgattca agaaggatct tggtaagcgc atattcgcca
    14221 aactcatcac gtttattcac aggcaacttt gagcgcatat aggcatcaac tcgagccatt
    14281 ggtccgccta tgcaatagag cgcatcttca tcactccaag ttgctccgag agattccatg
    14341 attcttcgct cttcaccaat ccaaatcggt tcgctatcga tgagagttcc gtccatatcc
    14401 catagaacag ctttaaaagg aaaatcgcta attagcattg aaatactttg cctcggggtg
    14461 atgaacgata atcgctgagg tgctctgctc aggatgtagt tgaaattcct cggataactc
    14521 aacaccaatt cgctttggct ctaataattc acagagttga acttgtgcgg ccagatctgg
    14581 acaagctgga taaccaaaac tatatctaga gccgcgatag ccctgatcaa gaatctcgga
    14641 aagctcgcta ctatcatcac cagcaaaacc aagctcagat ctgatacgtg catgccagtg
    14701 ctcagcaaga gcctctgtta gctgaacgct aagcccatgg agttctagat attctcgata
    14761 ctcattggcc tcaaagagct ttgcagttgc ctggctaaca gattgcccca tcgtgaccac
    14821 atggaaagca acaacatcaa tctgccctga ttctttactc ttaaagaaat cacttaaaca
    14881 gagccttcta tctctccttt gacgtggaaa tgtgaatcga agtctctcgc tatctttctt
    14941 ctccccctca tgatgcaaga taactaaatc attaccctcg ctgtagcaag gaaaatagcc
    15001 atagacaacg gctgcattga tccatccttg agagactgca tcatttagca gagagcgaag
    15061 tcttggcctt ccctgatttt cgaccatctc ttcatattta ccgcgctcgg atttaagacc
    15121 ccattggcca acataaagtg ctcgctcatc aagcattgaa ctgtaatcag ccaaagcgat
    15181 tcccttaacc acccgagagc caaagaatgg cactttaggc acttggttat cagatgccac
    15241 atcacttctt gttgtatcag ttgtaagggg cgcatcaatt gatgtgactc tagttcgtcg
    15301 ctccttgaga ggtggaagtg aaactccttc aacacctttt ttaactccca tcatggtgtc
    15361 cattagcgag aggccctcaa aggcatctct tgcatatcga accgttcctg ggaatgcctc
    15421 tgctagatcc ttttcaacaa aggttctagt aagagcagct ccgccaagaa ttaccggcca
    15481 cttttctgcc aaacctcttc tttcaagctc ttctaaattc tccttcatta ttaccgttga
    15541 ctttactaaa aggccgctca tgccaataac atcaactgag gattgctcag cagcttcgat
    15601 tatttgatta atggtctgtt taattccaat atttacgacg cgataaccat tattggtcaa
    15661 gattatgtct accagattct taccaatgtc atggacatcg ccttttactg ttgccaggag
    15721 gatcgaaccg cgatcacttg catcactttt ttccatgtga ggttcaagta gagcaactgc
    15781 gctcttcata acttcagcag attgaaggac aaagggaagc tgcatatcgc ctcttccaaa
    15841 gagctctccc acttccttca ttccttcaag gagataatcg ttaataattg caagtggagc
    15901 aatgccgcca aggcgcgctt gatcaagatc agcttcaagt ccagccttct cgccatcaat
    15961 aattcttctt accaggcgtt gctctatcgg aagggctgca agttcggccg ctcttgattg
    16021 acgagttgag gtgacttcaa caccggcaaa gagctctaga agctttgata acgggtcata
    16081 gacacagtca tcaccttcaa attttcgccg gtcatagatt agatcaagcg ctacctctct
    16141 ctgttcctct gggattcgag agataggaga aatctttgag gggtgcacga ttgcgctatc
    16201 aagacctgcc ttaacagctt cggcaagaaa tactgagttt aaaacgatgc gagcagcagg
    16261 atttagaccg aatgaaacat tactaattcc aagtgtggtc tgaacttgcg ggaactcttt
    16321 ctttagttgc gcaatagcat taatagtttc aaggccatct ttacgggtct cttcttgtcc
    16381 ggtagcaatg ggaaaagtta aacaatcgat gatgatatcg gaggtcttaa tcccccattt
    16441 ttcatttaga tcctttatca aacgtcgtgc aactcgcaat ttccattctg cgcttcgagc
    16501 ttgaccttcc tcatcaattg ttagagcaat aagagctgcc ccatgttcct ttgcaatagg
    16561 catgattctt gcaaatctag aatcagggcc atctccatct tcatagttga ccgaattgat
    16621 tacacatcgc ccgccaagtg attcaaggcc tgccttaatc actgctggct ctgtggagtc
    16681 aagaacgatt ggaagtgttg ttgctgtcgc aaatctagag gcaagctcgc gcatatcttt
    16741 aacgccatcg cggcctacat aatcgaccga gagatcaagc atatgagctc cctctctaac
    16801 ctgatcttta gctatatcaa cacacttctg ccaatcttca gcaagaagag catctctaaa
    16861 ggcccttgaa ccattggcat ttgttcgttc gccaattgcg agatatgtgt tttgttgtcg
    16921 aaatggaaca aattgataga gcgatgatgc gccagcttcg tcggtaaaag ctctctcctt
    16981 caatgcagtt ccatgcactt ttctcactac cgctgcaagg tgctctggag tagttccgca
    17041 gcaacctccg ataaggccaa cagagtaatc tttaacaaat tgatccagtg agatagctag
    17101 ttcatctggg ccaagaggat agtaagcgcc cccatctttc aaaataggaa gacctgcatt
    17161 aggcataaca gaaataccaa cggggctgct cttacttaaa gttcttaaat gctcgctcat
    17221 ttcagcaggt cctgtagcgc aattcattcc aattaagtca acacctaaag attgcagaga
    17281 agatagagct gcagatatct cagatccaag gagcatggtt ccagttgttt caaccgtgac
    17341 ttgtgcaata attaaaatat ctttatcgca gttatcaacg gctgctctag cgccatttac
    17401 agcagctttt gcctggagaa gatcttgagt agtttcaatt agtagcgcat ctactccacc
    17461 atcaataaga ccccgagatg cttcttgata tgccgcacga agatcttcat atgtggtgtg
    17521 tccaagagtt ggaagttttg ttccagggcc aagggatcct agaacccatt taggttcacc
    17581 gcttgaaatg ctgaattcat ctgcaacgcc tctagcgata cgagcgccag cttcggctaa
    17641 ctcatagatt ctctctggaa tgtcatactc acctaagttc gccagattgg cgccaaaagt
    17701 atttgtttca actgcatcta ctcctgcgct gaaatattgt ctgtgaatat ctgcgattaa
    17761 ctctggacgg gtaatgttta aaacctcatt acagccctca agtccttgaa aatcatcaag
    17821 agaggggtta gcgctctgga tcatggttcc catggcgcca tctgcgatta ctaccctttt
    17881 gccaagggca aggcggagcg aactagcctc ttttaatttg gatgcgctca cttaagtgag
    17941 tttatcgtag gctctgcata tgcatctcag tgagagctcc tttaacctaa gaagccccat
    18001 tcttatcgct gcttttgaag gctggaatga tgctgctgag tctgcaacat ctgcaataac
    18061 ccatcttttg accatttggt cgcatcaaca aatcgctgtt gtagatagcg aggaatacta
    18121 cgattttcag gtcaataggc cgcagataaa aatcgatgag agcaatatca gagagattat
    18181 ttggcccgga accattgtct atgcggttag ttcgccagct cttgctaatg atttcttgat
    18241 tgttagaggg gtagagcctt caatgcgctg gaaaagcttt atttcagatc tattagatct
    18301 tgcagatgat tatgaagtct cgatgattat ctccattggc gcgctccttg cagatactcc
    18361 gcactcaagg ccatttaatg tcgcgataag cggggcacac ccagaggtct cggaacgttt
    18421 tgacctagag atttcaaagt atgagggtcc gactgggatt atcggggttc ttcaagatgg
    18481 cgcatttaga cgagggattg atgcaatatc tctctgggct gcagttccac attacgttgc
    18541 tactccccct tgtccaaagg cggcgctagc acttattaat gctctagagg attttcttga
    18601 aatctcgctg ccccaaggcg acctaccaga gagagcaaaa ctctgggaag cacaagttga
    18661 tcagatggca gctgatgatg cagaggttgg cgattatgta agacaacttg agagttcaaa
    18721 agattcagca gagctaccag aaatatctgg cgaaagtatc gctagagagg ttgagcgatt
    18781 cctgcgaaga aatcctggta tttagagagt aattccaagg taggtatcaa gggcccttga
    18841 gatctcgcca gggctgcctc caacttggcc tgcttcagtt tttgcacacc aattacttag
    18901 agccgcaaaa accctcggcg tatctaaatc atctgcaagg gcgtttacaa cttcttgaac
    18961 cacttcaaaa gtcggagcaa cttcttgcct tgataaagcg ctaagcaatc tatctagtaa
    19021 ctcagctgcg ctatctaata gctctttgct ccacattcga tcacttgagt atttatcagc
    19081 aaacaaagca aggcggatga cggctggaga aacaccttct tggcgaagct tggatacgaa
    19141 aactaagtta cctttgctct tactcatctt ctcgccatct aggccaatca ttcctgcgtg
    19201 gctgtaacaa cttgcaaact caactccagt gagagccttt gcttgaagag ctgtcatgta
    19261 atgatgaggg aatatcaaat ctgaaccccc tgcttgcaaa gttattgaag ttggacttgt
    19321 ctcaggaaga ttcttaattg caatcgcggt gcattcaata tgccaaccgg gccttccctc
    19381 tccaaatgga gcaatccagt aaggatcatt tcctgctcga gctttccata gcagtggatc
    19441 tagcccgtgg cgcttaccaa ctctttcggg atcaccgccc cgctctttaa agatagctag
    19501 cgccctctcg agtggcagtg gtaaatcttg gatcgctcca tcgacttgct gcaaatctag
    19561 atagatatct ccttcaagtt cataagtttt accggaagca accatctgct caacgagcga
    19621 gatgatttca gccattgatt caataacgcc gcgatatgca cttggtggta gcacgctaag
    19681 tgctgtcata tcctcacgga agagatctat ttgagatgcg gcgagctcct tccaatcttg
    19741 gctatcccgc gcagctcgtt ctaaaagtgg atcatcgata tcggttatat tttcaacaaa
    19801 tgaaactttt ttaccgtttg ctttcaaata tctattaacg agatcaaagg ctagataagt
    19861 ggccgcatgg cccatatgcg ttgcatcata aggagtaatc ccacaaacgt agatcgagtt
    19921 ctgctctcct gtgatctcaa cttttttgcc tttgtaagaa tcaaagagcg caagggttgg
    19981 atgattaatg ccagccaaag caggcggcat atagacatct ggccaagaga gcattgccct
    20041 agcctacaaa tctctgaagg cttttatcca cgattaaacc ggaggccacg gaaccgcggg
    20101 ccattgctca gatggataag gaaacccctc tgaaatcagc atttcaattc tctcaagaag
    20161 agattcaatt tcatctttag tgattagctc tccaagagag gcgccaatat caccgctaat
    20221 acgacgcttt agcgactcaa gcttgcttat ttcctcttta tccagttctt gtccagcgta
    20281 ctgccataac acggttctta atttgaactc ttcatggaag gttaagccat gatcacagcc
    20341 gagaatttct gactctgaaa tgggaagaat atgtccaaac tttctatctg tattattaat
    20401 aacaacatca aagagggcaa gatttcttat tgcaggcgtt gactgttggc caatggcgat
    20461 taaatccaga tttggatcta aatctatcca ctgctgaact gcgcccatgc caaaaggtcc
    20521 atctcgcatt acggttagag gaacacaatt aaattgccct acctcgctta taagatgcga
    20581 ggccacttct cttgaagcaa ggttgccatt cgcaaaatcc catagcgctc tctcgccagc
    20641 aatgggttta taaacaacag agaaactaga ttgatcactc gttaagactt tcccaaaaag
    20701 tgtggcattt gatgcatcga ccaatcgccc ttcaactatc aattctccgg aaagtagctc
    20761 ttctctacta agcatttaat tcattctggg acagatgtga ccatcaggat ttattggagc
    20821 tccgcagaac atgcaaggag gtcgtccagc tttaatcaca ctggatgctc gatcaatgaa
    20881 actgcgggcc tgtgatggag taatgcgaat tcttagaatc gctggtgctc cctcagtttc
    20941 atcgcttgct agctcatcaa agataacttc ctcgccttga tccccggtta tagcttgacc
    21001 ttcaaatcta atcatttggg aatctatctg ccaggttaga gccataaccc ctagagtaaa
    21061 ttcagaatct attggcatat caagtgcttc gttatctctc ggagctgctc cctcactgct
    21121 ttttacctct cgaagcatct ctttaaaacg atcagtaagt gcttgagctt gcgctttttc
    21181 aagggcaaat gagaaaacct gcccagcctt ctttacttga atgtagaact cccgctctcc
    21241 tggctgccca acagtgccaa gaatgaaccg atcaacttca tctaggttat agattcgtct
    21301 aagcgacatc ttttgaacct cccaaaatat tgccagctct tctctttttc tttttacttg
    21361 acttgatgga gacaatcttc tgattgaaag tcatcattat tcgactcttt gggccccaag
    21421 aaagtgtcgt taaagaacat ggatcgactg caaacttttg aaaattgtct aaacctgaac
    21481 ctactgtcag gcttgcagca atcttaataa catcaccatg ggtgattaga agaactgttt
    21541 cctttgggta ttttcgggtc aattctttga gtagggattc aatccttttt tcaagctcct
    21601 taaagctctc cccctgcgga aatctaaatg cagatggctt tgtctggata actttccaat
    21661 ctttcatccg gctcaattta gagagttttt ttccgctcca cgaaccgtaa ttcatttcaa
    21721 ttaagcgttt atcaatagat atccgctttt ttttggattt ggcaataccc tcgattgttt
    21781 caagacagcg ctgcatcggg cttgaaatta ttcgatcaat cttgagattt tcaaaagagg
    21841 agttaagttt cttgacctga gctattccat ctttagatag cccgattccc tctaactgcc
    21901 ctgcaagtat tcctgcattg ttggctactg aatgggcatg acgaagaaga aggaaagtag
    21961 tcatagcctt aagattatct ctttaaagct tccacttctt ttcgaagtat ctttgctagg
    22021 gtcaacacat ggagttgcgc cagttaggtc gtagtggggt cagggtctct cgccttggcc
    22081 taggaacgat gacctggggt cgggatactg atgagcatga agcggcagaa caactgcgtt
    22141 tctacttaga tgctggcgga aatttcattg ataccgcagc tgtttatggc aatggtgatg
    22201 ccgagcgagt tctaggtgga tttctagggg ttatggtccc aagggatcag atcatcatcg
    22261 caaccaaggc tgggatttct tttaaaagtg gagaaagagt tgtagataac tcaagaactt
    22321 cacttattgc agaccttgat aattctctat cgcatttaag aacagaatat gttgatctct
    22381 ggcagattca cacctgggat gaaaaaacgc cgctagaaga agtattgagc gctcttgact
    22441 acgcggtgag tactggaaga gctaggcatg taggaatctc taattttgct gggtggcaat
    22501 tagcaagagc agtgacgctt cagaatccaa tctttggtaa agctcccctt atcagcactc
    22561 agaatgagta ctcattactc aataggaaaa tagagagaga gattttgcca gcatctaggg
    22621 agctcggcgt tggagttctt gcctggtctc ccctaggtcg cggagtatta actggcaaat
    22681 atcgatcagg tcttccctct gattcaagag gagcctcgcc tcacttctca tccttcattg
    22741 agccatattt agacgagcgc tcaagaaaga tagttgaagc ggtaatggtt gcagccgatg
    22801 gtcttggatt atcgccactt gaggtttcac ttgcctgggt aagagattgt cctggagtca
    22861 cttcggcaat tatcggagca agaaccggag cgcagctacg cggagcgtta agcgctgagt
    22921 cagtgactct gccagtacca gttcgcgaag cacttgatga gataagtgct atctaggcat
    22981 taacgaggaa gtgaaagaga gttcgtgcgc caaattgcag tgaatctatt ggcactctct
    23041 catcaattcc atgaaataac ccagtgaaat ctagatctgg cggcagctta agcggactaa
    23101 agccataacc aactattcca agttttgcta aggctttatt atcagttcca ccacttagaa
    23161 gatatggaat tggaattcca tctggatctt ctgacttaat cgccgcaatc atggcatcaa
    23221 cgagtggtcc gccaaattca acctctaacg ggatatcaga gaccaggctt tcaaccttga
    23281 catgctcgcc gaccaatgat ttaacagtgt cgagaagctc tttttcatag cctggcaacg
    23341 ttctgccatc aacgactgcg cttgcacttt gcggaatcac attggctttg tagccagctt
    23401 caagcatcgt gggatttgta gtgttacaaa gcgttgcgcc aatcatcttg cccatcgggc
    23461 caacttcatc aattaacggc tggagattat ttctatcata tggctttcca gaaagctctg
    23521 agatcttctg aaagaaaaga tcactggttt tagtaattct ctgtggccag gtgtaattac
    23581 caatctttgc tatcgcctct gttaggcgag ttacagcatt tgcattgtta accatcgagc
    23641 catgacctgc aacgccctcg gcagttaact tcatccattc aattcccttt tgagaagctt
    23701 caattgcata aacgcgctta cccgagagga gagttagaga gaaacctcca acttcagaaa
    23761 tcgtttcgct gcatccagca aatacctctg gatgtttctc agccatccac cttgatccat
    23821 atatgccgcc agcttcttcg tcgccaaaga agacaatcac tatggtgcgc tctggttgat
    23881 agttatatct agcccaaagt cttatcactg caagaatcat tgcgtccata tttttcatat
    23941 caacagcgcc gcgtccccag atgcatccat ctttgattgc tccagagaat ggatctaccg
    24001 accaatcagc tgcatttgct ggaaccacat caagatgacc atttacgaca agtcccggtt
    24061 ttgatttatc tttgccttca attcttgcaa ctactgagca gcgattagga gcgctttcat
    24121 aaatcttgct ctgaattcca acttctttca tcttggcagc cacatattca gcagcagctt
    24181 tctcatcgcc ttttccttcg ccaaaattga cgcttggaat ctgaatcaat tcctggcaga
    24241 gctttactac atcttcttct agttctttgc gctgggcatc agttagtttc atgggctaaa
    24301 gtatgcccgc attgtccggg tggcggaatt ggcagacgcg ctagcttgag gtgttagttc
    24361 ccgcaaggga ttaggggttc aagtcccctc tcggacacgt ttta
//
LOCUS       DAOROD010000017        21716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_80_length_21716_cov_31.350215, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000017 DAOROD010000000
VERSION     DAOROD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 21716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21716
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80_length_21716_cov_31.350215"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..384)
                     /locus_tag="PLW53_04405"
     CDS             complement(1..384)
                     /locus_tag="PLW53_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HRD36696.1"
                     /translation="MSVLFSELLIPGWAEGEGAMIGLDAVTGTYGSDQAFSLPCHTSN
                     PEIFFSDKSDEIAYAKSLCSACPVKKECLQGALSRKEPCGIWGGELFEDGVVIAAKRL
                     PGRPRLNQEVVTSELIDEKVLVATA"
     gene            complement(494..637)
                     /locus_tag="PLW53_04410"
     CDS             complement(494..637)
                     /locus_tag="PLW53_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36697.1"
                     /translation="MTAARSTSLTMSAYRTHRNWSMPAFGYKYFATKSTKADGFGTWS
                     AIG"
     gene            complement(682..2307)
                     /locus_tag="PLW53_04415"
     CDS             complement(682..2307)
                     /locus_tag="PLW53_04415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRD36698.1"
                     /translation="MAQLADEILSSLDPQQRSVATALKGPVCVIAGAGTGKTRAITHR
                     LAYGVDIGVIDPKRVLALTFTARAAGEMRTRLRSLGVASIAARTFHAAALKQLIYFWP
                     ETLGGRFPSLLTSKTGFLSEAMTRANISSSKKSSLLRDLAGEIEWAKVLTLAPDQYLQ
                     GLSDYSRVFNPSSKISAEQVAKVYEAYESLKKQERAIDFEDVLLLTVGMLEEEREVRE
                     RVRDQYRYFTVDEYQDVSPLQQRLLDLWLGKRDDICVVGDPAQTIYSFAGASPAFLLN
                     FTAKYPNAEVIRLSAGYRSTPEIINTANTILRSANLGHELDAINGHGEKPMAKGYKSQ
                     NEEAQALVSLIKEDIAGGLATNEIAILTRTNSQLEVLESALDAAGIENQIRNSERFFN
                     RPQVREIIGAIRSASVLSESDWLSDLRDCLKPFGQSEYVRSFLQLAGELEAEGLTSLR
                     AFLRELEERSETNNPPILPGVALATLHAAKGLEWRKVYLAGVSAEVLPWQASSIEEER
                     RLFYVGVTRAQESLALTYYGVASPFLAEAGLVN"
     gene            complement(2295..3194)
                     /gene="nudC"
                     /locus_tag="PLW53_04420"
     CDS             complement(2295..3194)
                     /gene="nudC"
                     /locus_tag="PLW53_04420"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRD36699.1"
                     /translation="MLLNLPLSRSKLDRAAHLRSDSASLDELFSKAKIISMVADRFEC
                     SQSGLTFIPPELAKGERYFLGLSSSGEPYFVVHQVSDQKEFGENFQSLRTLGAHLNDL
                     EIGAAVHSLALSQWHQSHMRCSKCGEETKSILGGSVRKCLVCSAEHHPRTDPAIIVLV
                     KDSKDRILLGRQKVWPEKRFSTFAGFVEPGESFESCVIREVAEECGGIVTEMKYLGSQ
                     PWPFPASVMIAYEAVISNSDLVVADGQEIEEIVWLTREELKSKCESGELLLPPIISVA
                     RAMINAWYGPAAESELSGQSWRN"
     gene            3220..4026
                     /locus_tag="PLW53_04425"
     CDS             3220..4026
                     /locus_tag="PLW53_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRD36700.1"
                     /translation="MASTQGFSLTCWNTLHGMAIPPNSGATLQSAVVQMLQEVSSDVI
                     TLQEVDLNQDRSAGVNQVSHIAKLIGANYWAFAPSLIGTPGEKWSAVESELIYTQDCV
                     IPNQAMYGIGIVSKVKVKSWHRLNLGRSAIGMPLLVPGEKRAQFIYVSDEPRSALVAE
                     LENGLSISTTHLSFVPGKNVTQLRKIIKWLPSISGKHILTGDFNLPWGVAAKISGWRD
                     LAPGATYPSWKPNIEFDYILSKDIDADQVKPLIHKHNGLSDHRALSITIS"
     gene            complement(4023..4565)
                     /locus_tag="PLW53_04430"
     CDS             complement(4023..4565)
                     /locus_tag="PLW53_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36701.1"
                     /translation="MSYFNLSDYEPVENRIRAFWLKYPNGRILTDLQRTERSDGRIEW
                     ICRSEAYTNSEDIRPQSTGFATEIEGTSPVNRANASENCETSSIGRCLANLGFAAKGK
                     RPSREEMAKAARGSSGKVLNKSDWERLIRELHGCTELSQLKAWSAYAASFNMPEVKRN
                     ELLSIYKSHRESLSKKSEVA"
     gene            complement(4565..5062)
                     /locus_tag="PLW53_04435"
     CDS             complement(4565..5062)
                     /locus_tag="PLW53_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36702.1"
                     /translation="MNLRELAFQLSAITLIADAAKEAKDRLRRQFAEALEEVGADSAK
                     AALEGEEIAKVSLIRPKNTPQVLNEKAFVDWVKSNYEFEIIESIRESFRKHVMDSVEN
                     VDGKAIYKRSGEILDFITFNSRDSYISTRFLSGGREVLSQAFRSGSLTPSSVMAEELE
                     MAVGQ"
     gene            complement(5158..7077)
                     /gene="typA"
                     /locus_tag="PLW53_04440"
     CDS             complement(5158..7077)
                     /gene="typA"
                     /locus_tag="PLW53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRD36703.1"
                     /translation="MSWNLGSMSRVIKSAHLNGLEVKSREELRNLAIIAHVDHGKTTL
                     VDAMLWQSGAFAAHKAQADSQDRLMDSMDLEREKGITILAKNTAVKRGDSIVNIIDTP
                     GHADFGGEVERGLEMVDGVALLVDASEGPLPQTRFVLRKALEKNLPVILIINKVDRPD
                     ARIAAVVDEAYALFLDLDADESQIDFPIVYTSAKAGRASLTRPSDGGMPDEENLDVLF
                     DVIFKTIPAPKYHVGAPLQAHVTNLDSSPYLGRLALCRVHEGEINKGQMVTWIKTDGT
                     QSKVKISELMITEGLERVPATKAGPGDIIAVAGIETITLGETLADPENPYPLPLITVD
                     EPSISMTIGINTSPLAGRVGKKLTARLVKNRLDQELVGNVSLRILPTERPDTWEVQGR
                     GELQLAVLVEMMRREGFELTVGKPQVVTKMVDGKLHEPMEQLTIDAPEDYLGVITQLM
                     ALRKGRMEQMVNHGTGWIRMDFKVPSRGLIGFRTEFLTETRGTGLLHHVFDGYEAWHG
                     ELRTRNTGSLVADRSGTATSYAIFGLQERGTIFVEVSADVYEGMVIGENSRPEDMDVN
                     VVREKKLTNMRASGSDETEKLIPPKLLNLEQALEYCREDECVEVTPNTVRIRKVIFDQ
                     NERSRLTSKAKKANQ"
     gene            complement(7049..8122)
                     /gene="ychF"
                     /locus_tag="PLW53_04445"
     CDS             complement(7049..8122)
                     /gene="ychF"
                     /locus_tag="PLW53_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRD36704.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVV
                     GLPDERLAVLAKIFSSEKIIPAALSFVDIAGIVKGASEGAGLGNKFLANIRECDAICQ
                     VIRAFNDSDVVHVEGRVDPKSDIETINTELQLADLQTIQKAIPRIEKEARQAKERAVV
                     LEAVKEAEQVISSGGLISQSKIDLTLIRELQLLTAKPFLYLFNLDASELQDSELKARL
                     SALVAPAEAIFLDAKTESELIELDEAEALELLNSIGLSEPGLSSLARVGFATLGLQTY
                     LTAGPKEARAWTIHKGDTAPMAAGVIHSDFQKGFIKAEVVSFTDLVDAGSFNEAKARG
                     KVRMEGKDYVMADGDVVEFRFNV"
     gene            8174..9319
                     /locus_tag="PLW53_04450"
     CDS             8174..9319
                     /locus_tag="PLW53_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRD36705.1"
                     /translation="MNTAIYLLIGLSFGIGLGLLIGYFRLNSERGKVSLLNGQIEAMR
                     QEESRLTTLNEQLRSVTTSMAQLNTQSQEAEIKRTKAESEMRAQIENMKLGNETLLRE
                     TTKLAGALSNSQTRGKYGEAQLETLLENAGLLENVHFFKQDYRSSGSEITKPDIKIAV
                     PGGSELFIDSKFPFDRFLEAIVEKDPTERTRLMQAHAKDLMGHVTSLAKRGYQDSNNS
                     PDYVVLFAPFESILSEALDVDPQLLHKAFEKNVTIATPTTMMALLRTVAYVFSRSELA
                     RNAQEIQDLAGELLKRIGKVHSKIETLGDRIKSTERAFNDLIKSAEENVIKPARKMVK
                     LGAPSSSKLKAIEDIDDEVRQIAPKELGSGALDDGDDDLDEGESDER"
     gene            9309..9731
                     /locus_tag="PLW53_04455"
     CDS             9309..9731
                     /locus_tag="PLW53_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36706.1"
                     /translation="MRDSLPTPPIKSPIKGAGLTKPGVVILQFLAISFVALIEIFFRS
                     NVGFLTGLAIWASYYGALIYGRDGTTYVAVVNPPLAFGLAAILLLPSVGGASLSITRL
                     GVDLVSGLASVAPFLITGSIFGWWYYFKERKKLLSSGS"
     gene            complement(9711..10664)
                     /locus_tag="PLW53_04460"
     CDS             complement(9711..10664)
                     /locus_tag="PLW53_04460"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRD36707.1"
                     /translation="MAKRVLLAAPRGYCAGVDRAVITVEKALDLYGAPVYVRKQIVHN
                     IHVVSSLEKKGAIFVDETDQVPEGSIVIFSAHGVSPQVHKEAAQRNLKTIDATCPLVT
                     KVHQEARRFAKDDLQILLIGHEGHEEVEGTAGEAPDHVKLVDGLDGARSVEIDPNKGV
                     AWLSQTTLSVDETLDAVAILKERFPKLQDPPSDDICYATQNRQVAIKQIAPQADLVLV
                     VGSKNSSNSVRLVEVALEYGAKAGYLIDYASQLEDNWFEGVETIGVSSGASVPEILVT
                     DLLTELAARGYSDVETVTAMEEHLLFAIPPELRKDLRAAGK"
     gene            10690..11916
                     /gene="xseA"
                     /locus_tag="PLW53_04465"
     CDS             10690..11916
                     /gene="xseA"
                     /locus_tag="PLW53_04465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRD36708.1"
                     /translation="MLETSSEKPAPVRVISEAISEYINRLGQVWVEGEIAQLNDRAGS
                     GMVYIRLRDPSVDMYLEVTCPRSVFKAVAPLSENARVVINSKVNFYTPTGRLSLNAKE
                     IRQVGLGELLARLEALKKVLAAEGLFALERKKPLPFLPNKIGLICGRASAAEKDVKEN
                     ARRRWPAVRFETREVAVQGADAVIQVSAALRELDNDPEVDVIIITRGGGSFEDLLAFS
                     DEGLVRLAAACKKPIVSAIGHEQDTPLLDLVADVRASTPTDAAKLVVPDFQEEVSKIK
                     NLRDRSDRKINDLVILEMTKIVNLIQRPVMKDPLIMVTSRQEIISGFISRSFMSMNSK
                     LLRAFDEVTHIAARVRTLSPQATLDRGYSVVQKSDGVIVKDPASLAPGEKIQLRMAKG
                     VALATALGSQEDSKSE"
     gene            11926..12123
                     /locus_tag="PLW53_04470"
     CDS             11926..12123
                     /locus_tag="PLW53_04470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRD36709.1"
                     /translation="MAQLSYEQARDELANVVATLEAGGVGLEESLKLWERGEELAAIC
                     QQWLDGARAKLEAAKSQVEAE"
     gene            12127..13788
                     /locus_tag="PLW53_04475"
     CDS             12127..13788
                     /locus_tag="PLW53_04475"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRD36710.1"
                     /translation="MPEFIYEDLLPVGPDTTQYRLISSDGVSTFEGDGKTFLKVTPEA
                     IRNLTEVALHDISHYLRSEHLQQLANILKDPESSANDRFVALDLLKNANIAAGGILPM
                     CQDTGTAIVMGKKGQQVLTSEQDEVTISRGIYDAFTKLNLRYSQLAPVTTWEEKNTGN
                     NLPAQVEIYSDYKNAQEYNFLFIAKGGGSANKSFLYQETKAILNPTSFITWLEEKLRS
                     LGTAACPPYHLAIVIGGTSAEFTAKTAKLASTKYLDSLPTTGDAKTGRAFRDLALEKE
                     VLALTQKLGIGAQFGGKYFCHDVRVIRLPRHGASLPISIAVSCSADRQAKAKITKEGV
                     FIEVLERDPAHFLPETTDDHLDESVVNIDLNQSMAQILAELSKHPVKTRLSLSGTMVV
                     ARDLAHAKLKELVDSGKELPEYFKRYPVYYAGPAKTPKGYASGSFGPTTAGRMDSYVE
                     YFQALGGSLIMLAKGNRSKQVTEACKKNGGFYLGSIGGPAARLAQDCIKKVEVLDYEE
                     LGMEAVWKIEVENFPAFIVVDDKGNDFFNGSLKPLTIGVKPESSF"
     gene            complement(13785..14357)
                     /locus_tag="PLW53_04480"
     CDS             complement(13785..14357)
                     /locus_tag="PLW53_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRD36711.1"
                     /translation="MLLNRRAIVALAFATLFISSVEISHAQPLKPDRLELLVADSPEA
                     LKVSVPGDLEPNWPTLIEMSDRSSLMAWDMLLVSVMIQKVELARTPKGAREVTREMAK
                     EAYGWGSYQFSCLDTLWTKESHWNYKARNKRTGAHGIPQALPATKMEVVGTDWRTNPV
                     TQIQWGLRYIDIRYETPCKALAKFKRSRYY"
     gene            complement(14570..15892)
                     /locus_tag="PLW53_04485"
     CDS             complement(14570..15892)
                     /locus_tag="PLW53_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD36712.1"
                     /translation="MARASSEGRKTYVLDTSVLLADPSAFFRFDEHEIIIPIAVIGEL
                     ESKRDHPELGYFARAALRKLDDLRITHGRLDKPLKITATGGTLSVELNHTDTSSLPHG
                     FLRDGTNDSRILAVARNLVSEGKDVVLVTKDLPLRVKASSVGVSADEYRAELAPNSGW
                     TGMAEVSVGSKTIDALYSGEKVSAAEVKDLPVHTGVVLHSEKGSALARVNADKTLSLV
                     RGDKSAFGIHGRSAEQRIALDLLLDNEIGIVSLGGRAGTGKSALALCAGLEAVLERRL
                     HKKIIVFRPLYPVGGQELGYLPGSEGEKMSPWAQAVFDTLGAVVSAQVIEEILDRGLI
                     EVLPLTHIRGRSLHDSFVIVDEAQSLERGVLLTVLSRIGQGSRVVLTHDVAQRDNLRV
                     GRHDGVVAVVESLKGHPLFAHVTLTRSERSQIAALITELLEDPVQLYA"
     gene            complement(16034..16822)
                     /locus_tag="PLW53_04490"
     CDS             complement(16034..16822)
                     /locus_tag="PLW53_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRD36713.1"
                     /translation="MRAKTLIKSALYPLYEKRLLAKLDLSRTPRHIAVILDGNRRWAK
                     ENPSLNGDTSSAHGHRAGASKIIELLTWCEQTEIDVVTLWLLSSDNLNRSPQELDSLM
                     QIIGSSVESLGETGRWSIKVLGSLELLPEKLQEQLKRIEKQSAGISGLKVNVAIGYGG
                     RLEIVDAIKSYLDSAKSSGKDLTQIRDELTVNSLDQYLYTAGQPDPELVIRTSGEQRL
                     GGFLLWQSHQSEFYFCEAYWPDFRRVDFLRALRAYSMRERRLGR"
     gene            complement(16819..17124)
                     /locus_tag="PLW53_04495"
     CDS             complement(16819..17124)
                     /locus_tag="PLW53_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRD36714.1"
                     /translation="MNGVIKRYRFMAVAAGIMSLLLWFVELPVVYLLDNPDLESKVKW
                     IPFVHGYIYAFYVLAAIHFTARARFTIKRMILFILAGTLPIASFIAERRVMRQFPLS"
     gene            complement(17108..17821)
                     /locus_tag="PLW53_04500"
     CDS             complement(17108..17821)
                     /locus_tag="PLW53_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRD36715.1"
                     /translation="MMKISKRELPSKVGAAFIVLILCATFIRLGFWQLDRAGEFQELQ
                     KPYIERPIINLSQVAIPGENLSDDSINQIVRFSGRYLEQYIAPNQEDKSGVKADWIVG
                     LLEVDSGGAILVVRSISNSELPIGSVEITGRLFNRQFDDRAEKASSKLSRIDPALLVS
                     SYEQKLFDGFVLAQSEKINGVEIDLKRPELDPAKPNVPGYYWQHISYVVIWWLMALVV
                     LFLPFYQVSRKRQSYERSN"
     gene            complement(17805..17948)
                     /locus_tag="PLW53_04505"
     CDS             complement(17805..17948)
                     /locus_tag="PLW53_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36716.1"
                     /translation="MEATVYDAGIAGSMTMIFLTLATVFLLRSFYKRWKRMDQRRLND
                     ENK"
     gene            17963..18379
                     /locus_tag="PLW53_04510"
     CDS             17963..18379
                     /locus_tag="PLW53_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HRD36717.1"
                     /translation="MSQQIGGNEEFLAQRYGKKGSNTWRYIAALLLLTALPWLIWSAW
                     HHSNPQIRVTLISFQNEQDNSIDITYLIQRRDPSLALNCTLIARDFDKNVVGEVEVAI
                     PSSSQGSLSRNDTIPTRLRAVNASVQKCDAIDLKGK"
     gene            18391..18864
                     /gene="greA"
                     /locus_tag="PLW53_04515"
     CDS             18391..18864
                     /gene="greA"
                     /locus_tag="PLW53_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRD36718.1"
                     /translation="MSKSWLTQEAADRLSAELANLETTGRSEIIKKIEAARAEGDLKE
                     NGGYHAAREEQGKIEARIRQLKHMLENAEIGTAPNNADGLISPGMRVTVEIMGDEMEF
                     LLGSREISSGDLDVYSEKSPLGAAVMGAKIGDTKTYNAPNGKTISVKILKAEVYS"
     gene            complement(18865..19668)
                     /locus_tag="PLW53_04520"
     CDS             complement(18865..19668)
                     /locus_tag="PLW53_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36719.1"
                     /translation="MIKYAVSDSLIITKRQLLQLIRVPEVLIFSTIQPVMFVLLFRYV
                     FGGSISTGIPGVDYVQLLMPGIFVQTVAFTLAGTAVGLAEDMQKGLIDRFRSLPISRS
                     AVVFGRTLGDGALNIVVLAVMGLAGYAVGWRPSSGLFNVLLGFAFLLLFGFAMAWVGV
                     LIGLSVRSARVANAAVFIAVFPLTFLSNAFAPTTGMPKALQYFAEWNPVSTMVAGCRE
                     LFGLQNIFGVTANSWPSQNPLEMSLIYMVIIMAIFIPLSVRKYINTASK"
     gene            complement(19665..20657)
                     /locus_tag="PLW53_04525"
     CDS             complement(19665..20657)
                     /locus_tag="PLW53_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36720.1"
                     /translation="MKAVIAENLIKTYDKGKVRALDGLSLDVEEGTVLGVLGPNGAGK
                     TTTVRILATLLTPDSGNASVAGIDVIKDPGKVREVIGLSGQYAAVDETLTGWDNLTMF
                     SRLYHLSPAASKKRATELLEQFSLTDAAKRPIKTYSGGMRRRLDLAASLIVKPKILFL
                     DEPTTGLDPRGRIEMWEVIQGLVKDGTTLLLTTQYLEEADQLADEIAVIDQGKVIARG
                     TSDQLKKEVGGERLEIVAEAKDLKQVAEIVGKVSGGKVLVEEGSRQVSAPVTTGSRAL
                     IEAVRSLDDLGIHPLDIALKRPSLDDVFIALTGHVAEEKKSEELEAAQKRGRRK"
     gene            20794..>21716
                     /locus_tag="PLW53_04530"
     CDS             20794..>21716
                     /locus_tag="PLW53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36721.1"
                     /translation="MAVGFGLIIPAIPIFAKSFGVSNTLIGFIISAFAIMRFISGLFA
                     GKLVDRFGERLVLGVGLFMVSVFTLISGLAQNYEQLLIFRTAGGLGSSMFSVSAGALI
                     LRVVSDSQRGQAQSLYNGGFIAGGVAGPAFGGALIALSPRAPFFIYSGLLIVAGTVSL
                     IFLHESRLGAKVEQSKEERALTIKEALAIKPYLYSLFLAFLGSWILFGMRSSILPLFV
                     VEDLKASPSLVGLSFTVALIAQGSVMVRAGKHSDKNGRKPVILTGFSIVMASLGILFL
                     ANNVTVYLIAMIVMGLGAGFAASAGAIVGDV"
BASE COUNT         6010 a         5092 c         4623 g         5991 t
ORIGIN      
        1 ttaagcagtt gctaccaaaa ccttttcatc aattaattca gaagtgacaa cttcttgatt
       61 taatctcggt cttccgggaa gtcttttggc agcgataacc actccatctt caaatagctc
      121 cccgccccaa ataccgcaag gttcctttcg cgaaagagct ccttgtaagc actccttctt
      181 taccggacat gcactgcaga ggctcttggc ataagcgatc tcatcgctct tatcagagaa
      241 gaagatttca ggatttgaag tgtgacatgg caagctaaat gcttgatcgc tgccgtatgt
      301 gccagtgact gcatctagtc cgatcatggc gccttcgccc tcggcccagc ctggaattaa
      361 tagttcgcta aagagaacgc tcatcgttct cacctctgcc ttcctttttc atctcatttt
      421 taattacctg gataaagaaa aaggggcccg aatccttttg gattctaggc ccctgggctt
      481 tgcttaatcg gtgttatccg atagcgctcc aggtgccgaa tccatcggcc ttggttgact
      541 tggttgcaaa atacttgtaa ccaaatgcag gcatgctcca attgcgatga gtgcgatatg
      601 cgctcatcgt tagtgaagta cttcttgctg ctgtcatggt ttgcgaagag taatgcatcg
      661 ctagcaaatg atgcaaacta attaatttac taacccagcc tctgctaaaa atgggctcgc
      721 tactccgtag taggtaagag caaggctctc ttgcgcccga gttaccccca cataaaacaa
      781 acgtctctct tcctctattg agctagcctg ccaggggaga acttcagcgc taactcctgc
      841 gagataaacc ttgcgccact ccaaaccctt tgcagcatgc agcgttgcaa gagccactcc
      901 aggaaggatc ggggggttat tagtctctga gcgctcttca agttctctta gaaatgcccg
      961 aagagaggta agtccctctg cctcaagttc tcccgctaac tgcaaaaatg agcgtacata
     1021 ttcactctgc ccaaatggtt ttagacaatc tcgcaagtca gagagccaat cagattctga
     1081 tagcactgaa gcgcttctta tcgctccaat tatctctctt acctgcggcc tattaaagaa
     1141 gcgctctgaa tttcttattt gattctcaat acctgcagca tcaagagcgc tctctagaac
     1201 ctccaattga ctatttgttc tagtaagaat tgctatctca tttgttgcaa ggccgcctgc
     1261 gatatcttct ttaattagtg aaactagcgc ctgcgcctct tcgttctgtg atttatagcc
     1321 cttcgccatt ggcttctcgc catggccatt tatcgcatct agctcatgtc caagattggc
     1381 gcttcgcaaa atagtatttg cagtattaat aatttcagga gttgagcggt atccagcact
     1441 cagcctaatc acctcagcgt ttggatattt cgcagtgaag ttaagtagaa aggctgggct
     1501 tgctccagca aaagagtaaa tcgtctgagc tggatctcct acaacgcaga tatcatctct
     1561 ctttccgagc cagagatcta atagtctttg ttgtagcggt gatacatctt gatattcatc
     1621 aactgtgaag tatctatatt gatctcttac cctctcccta acttctcgtt cttcctcaag
     1681 cattccaaca gttagtagta atacatcttc aaaatcaatg gcgcgctctt gcttctttaa
     1741 tgattcataa gcttcataaa cttttgccac ctgctcagcg cttatcttgc tacttgggtt
     1801 aaagactctg gagtaatcgc tgagtccttg caggtattga tctggggcca gggttaaaac
     1861 ttttgcccat tcaatctctc ctgctaaatc tcgaagcagc gaacttttct tactacttga
     1921 gatatttgct cgcgtcattg cctcgcttaa aaaaccagtt ttactagtta ataacgaggg
     1981 aaaacgcccc ccgagcgtct caggccagaa gtaaattaac tgctttaggg ccgccgcatg
     2041 aaaggtcctt gccgcaatac ttgcaactcc cagtgatcta agcctggttc gcatctcacc
     2101 tgctgccctt gcagtaaatg tcagagccag aactcgttta gggtcaataa ctccaatatc
     2161 aacgccatag gccaggcgat gagttatcgc tctcgttttt ccagttccag ctccagcaat
     2221 tacacatact ggccccttta gagcagttgc aacacttcgt tgctggggat caagtgatga
     2281 aagaatctca tcagctaatt gcgccacgac tgacctgaca attcactctc tgcagctggg
     2341 ccataccaag cattaatcat cgctcgagca actgaaataa ttggtggcag cagtaactcg
     2401 ccgctttcac attttgactt taactcttct cgagttagcc aaactatctc ttcaatctct
     2461 tgcccatctg ccacaactaa atcagaattt gaaatcactg cttcataagc aatcatcact
     2521 gatgctggaa atggccatgg ctgtgagcca agatatttca tctcagtaac tatcccgccg
     2581 cactcctcag caacttctct gataacacaa ctttcaaatg attcacctgg ttcaacaaaa
     2641 cctgcaaaag ttgagaagcg cttctctggc cagactttct gtctcccaag caaaattcga
     2701 tctttactat ctttaaccaa aacaataatc gccgggtccg ttcttggatg atgctcagcg
     2761 ctacagacca aacattttct tacagagcca ccaagaattg atttagtctc ctcgccgcat
     2821 ttagagcatc tcatatggct ctgatgccac tgcgataagg caaggctatg aactgctgcc
     2881 ccaatttcta gatcatttag gtgagcacct agcgttctta aagattgaaa gttctctcca
     2941 aattcttttt gatcactcac ctgatgaact acaaaatatg gctcgccgct acttgaaagc
     3001 cccagaaaat atcgctcacc cttagcaagc tcgggcggga tgaaagtcag cccactctgg
     3061 ctacattcaa agcgatcagc gaccatagaa attatctttg ccttggaaaa gagctcatca
     3121 agtgatgcag aatcactgcg caagtgagcc gctcgatcta gctttgagcg tgagagcgga
     3181 aggtttagca gcacggggcg accctactag cctgccttca tggcatctac ccagggcttt
     3241 agcctgacct gctggaacac ccttcatggc atggcaattc cgccaaattc tggggcaacg
     3301 cttcaaagcg cggtagttca gatgctccaa gaagttagta gtgatgtaat cacgcttcaa
     3361 gaagttgatc tcaatcaaga tcgatcagct ggagttaatc aggtaagtca tatcgctaaa
     3421 ttaattggcg caaattattg ggcctttgct ccatcactga ttggaacacc cggtgagaaa
     3481 tggagcgcag tagaaagtga attgatctac acgcaagact gcgtgattcc aaatcaggcg
     3541 atgtatggca ttggaattgt ttcaaaagta aaagttaaat cctggcatcg cctaaatctt
     3601 ggaagatcag ctatcggtat gccgcttttg gttccaggcg aaaaaagagc gcaatttatc
     3661 tatgtctcag atgagccaag gagcgctcta gttgcagagc ttgaaaatgg tctttcaatt
     3721 agtaccactc atctttcatt tgttccaggc aagaatgtca ctcagcttag aaagataatt
     3781 aaatggcttc caagtatttc tggtaaacat atcctcaccg gagactttaa tcttccttgg
     3841 ggagttgcag caaagatctc tgggtggcga gatcttgccc caggtgcaac atatccatca
     3901 tggaagccaa atatcgaatt tgattacatc ttaagtaaag atattgatgc agatcaggtt
     3961 aagcctttga ttcataagca caatggctta agtgatcaca gagctcttag cattaccatt
     4021 tcttaagcaa cttcagattt tttactcaag ctctctcgat gactcttata gattgaaagt
     4081 agttcatttc gtttaacctc tggcatatta aagcttgcag cataggcaga ccaggctttt
     4141 aattgactta actcagtgca accatgaagc tctctgatga gccgctccca atcactctta
     4201 tttaagacct ttccagagct acctctggca gcctttgcca tctcctccct acttgggcgc
     4261 tttcccttgg cggcaaaacc taaattagca aggcagcgac caatacttga agtctcgcaa
     4321 ttctcactgg cattagcgcg attaactgga gaggtgcctt caatctctgt agcaaatcca
     4381 gttgactgcg ggcgaatatc ttcagagttg gtgtatgcct cactgcgaca gatccattca
     4441 attctgccgt cgctgcgctc agttctctgc agatcagtca agattcttcc atttggatat
     4501 ttgagccaga acgctctaat gcgattttca actggttcat aatcacttaa attaaaatag
     4561 ctcattactg ccccaccgcc atctctagct cttcagccat aacagaactt ggcgttaatg
     4621 atccgcttct aaaagcttgg cttaaaacct cacgaccacc gcttaaaaat cttgttgaga
     4681 tataagagtc acggctatta aaggtaatga aatccaagat ctcaccgctt cgcttataga
     4741 tcgctttacc atcaacattt tctaccgagt ccatcacatg ctttctaaat gactctctaa
     4801 ttgattcgat gatttcaaat tcatagttag atttaaccca atcaacaaag gctttctcat
     4861 ttagaacctg cggggtattt ttaggtctaa ttagtgagac cttagcaatc tcctcacctt
     4921 caagtgcagc tttggctgaa tctgcgccaa cttcctcaag agcctcggca aattgccttc
     4981 ttagcctgtc cttggcttct ttagcagcat cagcaataag tgttattgct gagagttgaa
     5041 aggctagctc tcgtagattc atctgtaata cctccggctt atttgcttat ggaggtgatt
     5101 aaagaggcaa ccactgacaa tgggccaaaa gtgcctataa atcagcgtgt ttagccctta
     5161 ttggttagct tttttagcct ttgaggttag gcgggagcgc tcgttttgat caaagatgac
     5221 ttttctgatg cgaaccgtat ttggagtgac ctcaacacat tcatcttcgc ggcaatactc
     5281 aagtgcctgc tctagattta atagttttgg cggaattaac ttctctgtct catcgctacc
     5341 tgaggcgcgc atattggtta gcttcttctc gcgcaccaca ttgacatcca tatcttccgg
     5401 ccttgagttc tcaccaataa ccattccttc atagacatca gcgcttactt caacaaatat
     5461 tgttccgcgc tcctgcagac caaagatggc atatgaggtg gcagttcctg atctatcagc
     5521 aacaagtgat ccagtatttc tagttcttag ttcgccatgc catgcttcat agccatcaaa
     5581 gacatggtga agaagtccgg tgccacgagt ctcagttaag aactcagttc taaaaccaat
     5641 taagccgcga gatggaactt tgaaatccat tcggatccaa ccagttccat gattaaccat
     5701 ctgctccata cgacctttgc gaagcgccat tagctgggtg ataacaccta ggtaatcctc
     5761 tggcgcatca atggttagct gctccatcgg ttcatgaagt tttccatcaa ccatcttggt
     5821 aacaacttga ggctttccaa cggttagctc aaatccttcg cgtcgcatca tttcaactag
     5881 aactgcaagt tggagctctc ctcttccctg cacctcccag gtatcaggac gctcggttgg
     5941 aaggattcta agtgaaacat ttccaaccag ctcttgatca aggcggtttt tgactaagcg
     6001 agctgttaat ttcttaccaa ctcttcctgc aagtggggag gtattaatac caatagtcat
     6061 agagatactt ggttcatcaa cggtaattaa cggcagtgga tatggatttt caggatcagc
     6121 tagcgtttca cctaaggtaa tagtctcaat tccagcaaca gcgataatgt ctccagggcc
     6181 agcttttgtc gctggaactc gctcaagtcc ttcagtaatc attagttctg aaatctttac
     6241 cttgctctgc gtcccatcag ttttaatcca ggtaaccatc tggcccttat taatttcacc
     6301 ttcatggacc cgacatagtg caaggcgtcc aagataagga gatgaatcaa ggttagtaac
     6361 gtgagcttga agtggagctc caacatgata tttaggagct gggatagttt taaaaattac
     6421 atcaaagagc acatcaagat tttcttcatc tggcatgccg ccatcacttg gtcttgttag
     6481 cgatgctctt ccagctttag ctgaggtgta aacaattgga aaatcaatct gtgattcatc
     6541 agcatcaaga tctagaaaga gggcataggc ctcatcaaca actgctgcaa ttcttgcatc
     6601 agggcgatct actttattga taatcaaaat aactggaagg ttcttctcta gagcttttct
     6661 taaaacaaat ctagtctgcg gaagtggtcc ctctgaggca tcaactagaa gtgcaacgcc
     6721 atcgaccatt tctagtccgc gctctacttc gccgccaaag tctgcgtgcc ctggagtatc
     6781 aataatatta acgatgctat ccccgcgctt tactgcagta tttttagcaa ggatggtgat
     6841 tcctttttcc cgctctaaat ccattgaatc catcaatcga tcttgggaat ctgcttgggc
     6901 tttatgggca gcaaaagctc cagattgcca taacatcgca tcaacaagtg ttgtcttgcc
     6961 atgatctacg tgagcaataa tggctagatt tcttagctct tcacggctct ttacctcaag
     7021 gccatttaag tgggcagatt taattactct agacattgaa cctaaattcc acgacatctc
     7081 catcagccat tacgtaatcc ttgccttcca tgcgaacttt gccccgcgcc ttggcttcat
     7141 taaatgatcc agcgtcaact agatcggtaa atgaaactac ttcggcctta ataaagcctt
     7201 tctgaaaatc actgtgaatt acaccggctg ccataggagc ggtgtcgccc ttatgaatgg
     7261 tccaagcccg agcctcttta ggacctgcag ttagataggt ctgcaaccca agagttgcaa
     7321 aaccaacacg agcaaggcta cttaacccag gctctgatag tccaattgag tttaatagct
     7381 ctaaagcctc tgcctcatct aattcaatta actcagattc agtctttgca tcaagaaaaa
     7441 tcgcttcagc tggcgcaacc agcgcagata accgagcttt caactcgcta tcttgcagct
     7501 cgctggcatc aagattaaag agatagagaa aaggctttgc agttaatagc tgtagctctc
     7561 taataagtgt taaatcaatc tttgattgag atatcaagcc gcctgatgag attacctgct
     7621 ctgcctcttt tacagcttct agaacaactg ctctctcttt tgcctgcctt gcctcttttt
     7681 caattcttgg tattgctttt tgaatggtct gcagatctgc taattgcagc tcggtattga
     7741 tagtttcaat atcgctcttt ggatcaactc ttccttcaac atggacaaca tcgctatcgt
     7801 taaaagctct aattacttgg cagatcgcat cacactcacg gatattggca agaaacttat
     7861 taccaagtcc tgccccctct gatgctcctt taacaattcc tgcaatatca acaaaagata
     7921 gggctgctgg aattatcttc tcggaggaaa atatcttggc aagcactgcc aggcgctcat
     7981 ctggaaggcc aactacgcca acatttggct caatggttgc aaagggatag ttggcagcta
     8041 gaacgttatt tttcgttaaa gcgttaaata gggtcgattt tccgacattg ggtagaccca
     8101 cgattccgat ggagaggctc acaaggggta gagcctaact gccaatgtca cctcctgatg
     8161 ccacgatagc gccatgaaca cagcgatcta tctgctcatc ggcttaagtt ttggcattgg
     8221 cctcggactt ttgattggct atttccgcct caatagcgaa cggggcaaag tctcacttct
     8281 taatggccag atagaagcga tgcgccaaga agagagccgg ttaacaactc ttaatgaaca
     8341 gctaagaagc gttactacct caatggcgca gttaaatact cagagccaag aggcagagat
     8401 taagagaaca aaagctgaat ctgagatgag agctcaaatt gagaatatga agctaggaaa
     8461 tgagacgcta cttcgcgaga caactaaatt agctggagca ctttcaaatt cccagacccg
     8521 cggaaaatat ggcgaagcac aacttgaaac cctcctagaa aatgctggcc tattagaaaa
     8581 tgttcacttc ttcaaacaag attatcgaag cagcggtagc gagataacta aacctgatat
     8641 caagattgct gttcctggtg gcagcgaact atttatcgat tcaaagtttc cctttgatcg
     8701 attccttgaa gcaatagttg aaaaagatcc gacagagcgc accaggctga tgcaagccca
     8761 cgctaaagat ttgatgggcc atgtcacctc tcttgctaaa aggggctatc aagatagtaa
     8821 taactcacct gattatgtag tcctctttgc tccctttgaa tcaattctta gtgaagccct
     8881 cgatgttgac ccacaacttc ttcataaggc atttgaaaaa aatgtcacta ttgcaactcc
     8941 cacaacaatg atggcgcttc ttagaactgt ggcttatgta tttagtagat cagagcttgc
     9001 aagaaatgcc caagagattc aagatcttgc tggcgagcta cttaagagaa taggtaaagt
     9061 ccatagcaag atagagacgc taggcgaccg gattaaatca acggagcgag cttttaatga
     9121 tcttattaag agcgctgaag aaaatgtaat taagccagct agaaagatgg taaagcttgg
     9181 agctccatct agctctaaat taaaagctat tgaagatatc gatgatgagg taagacagat
     9241 tgcgccaaag gaacttggct ccggcgccct agatgatggc gatgatgatc tagatgaagg
     9301 tgagagcgat gagagatagc ctgccaactc ctccgataaa gagcccaata aaaggtgcgg
     9361 ggctaacaaa gcctggagta gtaattcttc aattcttggc aatatctttt gttgcgctaa
     9421 ttgaaatctt ctttagatcc aatgttggct ttctaactgg ccttgccatc tgggcttctt
     9481 actatggcgc gctgatttac ggtcgcgatg gaacaaccta tgtcgcagta gttaatcccc
     9541 cactggcctt tggtcttgca gcaatccttc ttctgccatc agttggcgga gcatcgctat
     9601 caattactcg tcttggagtt gatctagttt cagggcttgc aagcgttgcg ccatttttaa
     9661 tcactggctc aatatttggc tggtggtatt actttaaaga gcgaaaaaaa ctactttcca
     9721 gcggctctta aatcttttct tagctcaggc gggatagcaa agagcaggtg ctcctccatt
     9781 gcagtaacgg tctcaacatc gctatagcca cgagcagcaa gctcagttaa taaatcagtt
     9841 actaaaattt caggaactga agccccgctt gaaaccccaa tagtttcaac cccttcaaac
     9901 cagttatctt ctagctggct ggcataatca attagatatc cagcctttgc gccatattcc
     9961 agagcaactt caactagacg cactgagttt gaagagttct ttgaaccaac gactagaaca
    10021 agatcagctt gaggcgcgat ctgcttaatg gcaacttggc gattctgtgt tgcataacag
    10081 atgtcatcag atggcggatc ttgaagcttt ggaaagcgct ccttaagaat tgcaacggca
    10141 tcaagggttt catcaacgct aagtgtggtc tgggagagcc aagcaactcc tttatttgga
    10201 tcaatctcaa cgcttcttgc gccatctagg ccatcaacta atttcacatg atctggggcc
    10261 tcacctgctg ttccttccac ctcttcatgt ccttcatggc caatgagaag aatctggaga
    10321 tcatctttag caaaacggcg cgcttcttga tgaactttag taactagggg gcaggtggca
    10381 tcaatagttt ttagatttcg ctgcgctgcc tccttatgaa cttgaggtga gacgccatgg
    10441 gcagagaaga taacaattga accctctggg acctggtcgg tctcatcaac aaatatcgcg
    10501 ccttttttct caagtgatga aaccacatga atattgtgaa caatctgttt tctcacatag
    10561 accggggcgc catagagatc aagagccttc tcaactgtga ttacagctcg atcaacacct
    10621 gcgcaataac cacgcggcgc agcaagaagg actcgtttag ccatagccct atcctattag
    10681 cattacccaa tgctcgaaac ttcaagtgaa aagcctgccc cagtaagggt tatctctgag
    10741 gcgattagtg aatatataaa tcgcttagga caggtttggg ttgagggaga gattgcgcaa
    10801 ttaaatgatc gagctggctc tggaatggtt tatatcaggc taagagatcc aagcgttgat
    10861 atgtatttgg aggtcacctg tccaagatcg gtttttaaag cagttgcgcc tcttagtgaa
    10921 aatgctcgag ttgtaattaa ttcaaaagtt aatttctata ctccaactgg ccgtctctca
    10981 cttaacgcca aagaaatccg ccaagttggt cttggtgaat tacttgctcg ccttgaagca
    11041 cttaaaaaag tccttgcagc agaggggcta tttgcacttg agcgaaaaaa accgcttcca
    11101 tttctaccaa ataaaattgg actcatctgt ggtcgggcaa gtgctgctga aaaagatgtt
    11161 aaagagaatg ctcgaagaag atggccagcg gttcgctttg aaactaggga ggtagcagtt
    11221 caaggagctg atgctgttat tcaagtatct gctgccttgc gagagcttga taacgatcca
    11281 gaagttgatg tcatcatcat cactagaggc ggcggctcat ttgaggatct gcttgccttc
    11341 tcagatgaag ggctagtaag acttgctgcc gcttgcaaaa aaccaattgt cagcgccatt
    11401 ggccatgagc aagatactcc gcttcttgat ttagtagcag atgttcgcgc ctcaacccca
    11461 actgatgcag caaaattagt agttcctgat tttcaagaag aggtaagcaa aatcaagaat
    11521 ctaagagatc gctctgatag aaagattaat gacttagtaa ttcttgagat gaccaagatt
    11581 gttaacttga tccaaagacc tgttatgaaa gatccgctaa ttatggtcac ctcaagacag
    11641 gagataattt caggctttat atcgcgctca tttatgagta tgaattccaa gctgcttaga
    11701 gcctttgatg aagtcaccca tattgctgcc agagttagaa ccctctcccc acaggccacc
    11761 cttgatcgtg gttattcagt agtgcaaaaa agcgatggag ttattgtcaa agacccagca
    11821 tcacttgccc caggtgagaa gattcaatta cgtatggcta aaggtgtggc ccttgcgaca
    11881 gctcttggca gccaagaaga tagtaaaagc gagtagatta cgaatatggc ccagctaagt
    11941 tatgagcaag ctcgcgatga gctagcaaac gttgtagcaa cccttgaagc tggcggggta
    12001 ggccttgaag aatctttaaa actttgggag cgcggcgagg agcttgctgc aatttgtcag
    12061 cagtggctcg atggtgcacg cgcaaaatta gaagctgcaa aatctcaagt agaagctgag
    12121 taagtcatgc cggaatttat ctatgaagat ttacttccgg ttgggccaga taccacgcaa
    12181 tatcgcctca tttcaagtga tggcgtctca acctttgaag gcgatggaaa gactttctta
    12241 aaagtaacac ctgaggcaat tagaaactta actgaagttg ccctccacga tatctcgcac
    12301 tatctacgta gcgaacactt gcagcagcta gcaaatattc ttaaagaccc tgaaagctct
    12361 gccaatgatc gctttgtcgc actagaccta cttaaaaatg ccaatatcgc agcaggtgga
    12421 attcttccga tgtgccaaga caccggaact gcaattgtta tgggcaaaaa aggccagcaa
    12481 gttctcacct cagaacaaga tgaagtaaca atatctagag gaatctatga tgcatttacc
    12541 aagctaaatc tgcgctactc ccaattagca cctgtcacaa cctgggagga gaagaacact
    12601 ggtaataact tgcctgctca agttgagatt tactctgatt ataagaacgc gcaggaatat
    12661 aacttcctct ttattgcaaa aggcggcgga agtgctaaca agtctttcct ctatcaagag
    12721 actaaggcaa tcctaaatcc aacatctttc ataacttggc ttgaagagaa gttacgctcg
    12781 cttggaacgg ctgcttgccc tccctatcac ctagctattg ttattggtgg aacctcagct
    12841 gagttcacag caaagacggc aaagcttgcc tcaactaagt atttagattc actgcctaca
    12901 acaggtgatg ccaaaactgg tagagccttt agagacctag cacttgaaaa agaagtttta
    12961 gctctcactc aaaaactagg aatcggcgca caatttggcg gcaagtactt ctgccatgat
    13021 gtaagagtga ttcgccttcc aagacatggc gcctcacttc caatttcaat tgcagtctcg
    13081 tgctcagcag atcgccaggc aaaggcgaag atcactaaag agggcgtatt tattgaagtt
    13141 ttggagcgcg acccagcaca cttcctgcca gagactactg atgatcattt agatgagagc
    13201 gtagtaaata ttgatctaaa ccaaagcatg gctcagatct tggctgagct ttcaaagcac
    13261 ccagtaaaga cccggctatc cctgagcgga actatggttg tggcaagaga tctagctcat
    13321 gcgaaattaa aggagctagt tgattctgga aaagaactgc cggaatactt caagagatat
    13381 ccagtttatt acgcaggacc tgccaagact ccaaaaggat atgcctcagg atcttttggt
    13441 ccaacaactg ctggacgaat ggattcctat gttgagtatt ttcaagcact cggcggctcg
    13501 ctaataatgc ttgctaaggg aaatcgctca aagcaggtga ctgaagcatg caagaaaaat
    13561 ggtggttttt atcttggctc tattggagga cctgctgctc gccttgcgca agattgcatc
    13621 aagaaagttg aagttcttga ttatgaagaa cttggcatgg aggcagtttg gaagattgaa
    13681 gttgaaaact ttccagcatt tattgtggtt gatgataaag gaaatgattt cttcaacggc
    13741 tcactcaagc cgttaactat tggagtaaaa ccagagagct ctttttagta gtatctagag
    13801 cgcttaaatt tagctagcgc tttgcatggc gtttcatagc gaatatcgat atatctcaaa
    13861 ccccattgaa tctgagtaac tggattggtt ctccaatcag ttccaacaac ctccatcttt
    13921 gtggcaggaa gggcttgggg aattccatgg gcgccagttc ttttattgcg cgccttataa
    13981 ttccaatggc tctctttggt ccataaggta tctaaacagg agaattgata actgccccag
    14041 ccataagcct ccttagccat ctctcttgtg acctctcgag ctccctttgg ggttctggct
    14101 agttcaactt tttgaatcat cactgaaacc agcaacatat cccaggccat aagtgatgag
    14161 cgatcgctca tctcaattag cgttggccaa ttcggctcta gatctcctgg cacgctgacc
    14221 ttaagagcct caggtgaatc ggcaactagt agctctaggc gatctggctt gaggggttga
    14281 gcatgggaaa tctcaaccga ggagataaag agggtcgcaa aggccagggc aacaatcgcc
    14341 cgccggttta gcaacatctt tctacctcct tagcgctgaa caaatcagag ccaggctcgt
    14401 gggtattact aggggaattg ggcttagggt tacaaaggaa ggggtgaggg gcaaattaaa
    14461 acctgcgagt ggcgtaaaaa gattaaattc acagcaaatg tggataagaa aagtgcaggt
    14521 cagcccctat aaagtccgtt atgccctatt tgtagtaaaa ccccaattat taggcatata
    14581 gctggactgg atcttcaaga agctcagtta tcaaagctgc gatctgagag cgctcagagc
    14641 gggtcagcgt tacatgggca aagagaggat ggcccttaag agactcaaca acggctacaa
    14701 cgccatcatg gcgaccaacc cgcaggttat ccctctgggc cacatcatgg gtgagtacca
    14761 cccttgagcc ctgcccgatt cgagagagca cagttaataa aactccgcgc tctagagatt
    14821 gcgcctcatc gacaattaca aaagagtcat gcagtgagcg gccacggata tgagttaggg
    14881 gcagaacctc aataagtccg cgatctaaaa tttcttcaat tacttgggca gagaccacag
    14941 ctcctagggt gtcaaagact gcttgcgccc aaggagacat cttctcccct tcacttcctg
    15001 gcaagtatcc aagctcttgt cctcccactg gatagagcgg acggaagaca attatctttt
    15061 tatggaggcg cctttcaaga actgcctcaa gaccggcgca gagagcaaga gcagattttc
    15121 cagttccagc ccttcctccc agggagacaa ttccaatctc attatcaagg agcagatcaa
    15181 gtgctattcg ctgctcggca gatctgccat gaattccaaa tgcgctctta tctcccctaa
    15241 caagtgagag agttttatcg gcattaactc gtgcaagagc gctgcccttt tcagagtgaa
    15301 gcactactcc ggtgtgaact ggaagatctt taacttcagc agcgcttacc ttctcgcccg
    15361 aatagagagc atcaatggtt ttacttccaa cgctcacctc agccattcca gtccatccgc
    15421 tatttggcgc aagctctgcg cgatactcat ccgcgcttac tccaactgat gatgctttaa
    15481 ctcttaaagg gagatcttta gtaactagaa cgacatcttt tccttcactt actaaatttc
    15541 tagcaacagc caaaattctt gaatcatttg tgccatcccg caagaatcca tgaggcagtg
    15601 atgaagtatc agtatggttt aactcaactg agagagtgcc gccagttgcc gtgattttta
    15661 agggtttatc gagtcttcca tgggtaattc ttaaatcatc aagctttctt aaagctgctc
    15721 tagcaaagta tccaagctcg ggatgatctc gctttgattc aagttctccg attaccgcta
    15781 ttggaatgat gatttcatgc tcatcgaagc ggaagaaggc ggaggggtct gctaacagga
    15841 cgctggtatc tagaacgtag gtcttgcgac cttcggagct agcgcgggcc acggaactcc
    15901 ttgcttattc tgttgttata gggctttttc cgcctgaatt aggcagattt gccggggaag
    15961 taccacgaga aaaaggtaag tcctaaaggc tttgaaatca cgcgacacgc tcaagttcaa
    16021 cttgaggttt taactacctg ccgaggcgcc gctctctcat cgaataggcg cgaagtgctc
    16081 gaagaaaatc tacccggcga aaatctggcc agtaagcctc gcagaaataa aattcggatt
    16141 gatggctctg ccagagtaga aacccgccaa gtctttgctc cccagatgtt ctaatcacta
    16201 actcaggatc tggttgccct gcggtgtaga gatattgatc tagtgaatta acggttaatt
    16261 catctcttat ttgcgttaaa tcttttccag aactctttgc tgaatccagg tagctcttga
    16321 tcgcatcgac gatctcaagt cttccgccat agccaattgc tacattaact tttaggccag
    16381 atattcctgc gctctgtttc tcaatacgct ttagctgctc ttgaagtttt tcaggaagta
    16441 gttcaagaga gcccaaaacc ttgatactcc atctgccagt ttcgccaagg gactcaacac
    16501 ttgatccaat aatctgcatt aaagaatcta gctcttgggg gcttcgattt aaattatcac
    16561 ttgatagcag ccatagcgtc actacatcta tttcagtctg ctcacaccag gtcagtagct
    16621 caataatctt gctcgcccca gctctatggc catgagctga tgaggtgtca ccatttagtg
    16681 aaggattctc tttggcccat cttctattgc catctaaaat cactgcaata tggcgcgggg
    16741 tcctacttag atcaagcttt gcaagtaagc gcttttcata gaggggatag agcgctgact
    16801 tgattagagt tttagctctc atgagagggg aaattgcctc ataactcgcc gctcagcaat
    16861 aaatgaagca attggaaggg ttcctgccaa gataaacaaa atcattcgct tgatcgtaaa
    16921 gcgagccctt gcggtgaagt gaatagctgc aaggacatag aaggcataga tatagccatg
    16981 gacaaaggga atccacttaa cttttgactc taaatctgga ttatcaagga gatagaccac
    17041 tggaagctca acaaaccaga gcagaagcga catgatgcca gctgctaccg ccatgaagcg
    17101 gtatctctta attactccgt tcatagctct gcctctttcg tgatacttga tagaagggta
    17161 agaaaagtac taccaatgcc atgagccacc aaataacgac ataggagata tgttgccagt
    17221 aatagccagg cacattcggc tttgctggat ctagctctgg gcgctttaga tcaatttcta
    17281 ccccgttaat tttctcactt tgcgctagaa caaatccatc gaagagtttt tgctcatagc
    17341 ttgagaccaa tagcgctgga tcaattctgc tcaactttga tgaagccttc tctgctcgat
    17401 catcaaattg tctattaaag agccgtccgg tgatttcaac gctaccaatg ggaagttctg
    17461 aattagaaat acttcgcact accaatatcg ctccacctga atcaacttct aataggccaa
    17521 caatccaatc tgctttaact ccagatttat cttcttggtt aggggcaatg tattgctcaa
    17581 gatatcttcc actgaagcga actatttgat taattgagtc atcacttaga ttctcaccag
    17641 ggattgctac ctgcgatagg ttgattattg gtctttcgat ataaggcttt tgtagttctt
    17701 gaaactcccc tgcccgatct aattgccaaa agccaagtct tataaatgtt gcacagagaa
    17761 taagaactat aaaagctgcg ccaactttgc tcggcaactc cctcttactt attttcatca
    17821 tttaggcgac gctgatccat cctcttccag cgtttataga aacttctaag caagaaaact
    17881 gtggcaagag taagaaatat catcgtcatt gagcctgcaa tgccggcgtc ataaactgtt
    17941 gcttccatgt aatcattctc ccatgagtca gcagataggc ggcaacgaag agtttcttgc
    18001 ccagcgctat gggaaaaagg gctcaaatac ctggcgctat atcgcagcgc tgcttctgct
    18061 gacagctctt ccttggttga tctggagcgc ctggcatcat tcaaatccac agattcgcgt
    18121 tactttaata agttttcaaa atgagcaaga taattcaatc gatatcacct atttgattca
    18181 aaggcgagat ccatctcttg ccttaaattg cactttgatt gctcgggatt ttgataagaa
    18241 tgtggtggga gaagttgaag tagcaatccc tagttcgagc caaggctccc taagtcgaaa
    18301 tgacacgatt ccaacccggt taagggcggt taatgcttca gttcaaaaat gtgatgcaat
    18361 tgatctaaaa ggtaagtaac ctctgcgcct atgagtaaga gttggttgac ccaggaagct
    18421 gcagatcgac tatctgctga gctagcaaac cttgaaacaa ctggtcgctc tgagatcatt
    18481 aagaagattg aagcagcaag agctgaaggt gatctaaaag aaaatggcgg ctatcacgca
    18541 gctcgtgaag aacaaggaaa gattgaagct cgcatccgac aactaaaaca catgttggaa
    18601 aatgcggaga ttggaactgc gccaaataac gcagacggtt tgatctcacc tggcatgaga
    18661 gtaactgttg aaatcatggg cgatgaaatg gaatttctac ttggctcaag agagatttcc
    18721 tctggcgatt tagatgtcta tagcgagaag tccccgcttg gagctgcagt gatgggcgca
    18781 aagattggag atactaaaac ctataacgcg ccaaatggga aaactatctc cgtaaaaatt
    18841 ttaaaggcag aggtctacag ctaattactt tgaagcagtg ttaatgtatt tacgaacact
    18901 caaaggaatg aaaatagcca taataatcac catataaatc aaggacattt ccaagggatt
    18961 ctggctaggc caggaattag cggtaacgcc aaagatattc tgaagcccaa atagctctct
    19021 acatccagca accattgttg aaactgggtt ccattccgca aagtactgca gcgcttttgg
    19081 cattccagta gttggagcaa aagcatttga aaggaaggta agtggaaata ccgcaataaa
    19141 gaccgcagca ttggcaaccc gagcagagcg aactgaaagg ccaattaaga ctccaaccca
    19201 ggccattgca aaaccaaata gcagaaggaa tgcaaagcca agtaaaacgt tgaatagtcc
    19261 agaagatgga cgccacccaa cggcatatcc agcaagcccc ataactgcca acaccacaat
    19321 attgagtgcg ccatcaccaa gagttcttcc aaagacaaca gcagatcttg agattggaag
    19381 tgatctaaaa cgatcaatta aacctttctg catatcttca gcaagaccaa ctgcagttcc
    19441 tgccagggta aaagccactg tttgaacgaa gattccaggc ataagcagtt gcacataatc
    19501 caccccagga attccagttg agatggagcc accaaaaaca tatctaaata acaaaacaaa
    19561 cattactggt tgaatagttg agaaaattaa gacttcagga actcgaatta attgcaggag
    19621 ttggcgcttg gtgataatta gtgaatctga gactgcgtac ttgatcactt tctcctgcct
    19681 ctcttttgtg ctgcttctaa ctcttcagac ttcttctctt ccgctacatg gccagtcagc
    19741 gcaataaaga catcatcaag tgatggtctc ttaagcgcaa tatccagagg gtgaattccc
    19801 aagtcatcaa gtgagcgaac cgcctcaatt aaggccctag agccagtagt gacgggcgct
    19861 gagacttggc gagaaccctc ttccactaac acttttccgc cgctgacttt gccaacgatt
    19921 tctgccacct gctttaaatc tttggcctcg gcaacaatct cgaggcgttc tccgccaact
    19981 tccttcttta attgatctga agtgcctctt gcaataactt ttccttgatc tataacagca
    20041 atctcatctg caagctgatc agcttcctcc aagtattgag tggtgagaag caaagttgtt
    20101 ccatctttaa ctagtccttg aattacttcc cacatctcaa ttcgacctct gggatcaagg
    20161 ccagttgttg gttcatcaag aaatagaatt tttggtttaa cgataagaga ggcagcaaga
    20221 tctaatcttc ttctcattcc acctgagtaa gttttgattg ggcgcttggc agcatcagtt
    20281 agcgagaact gctcgagtaa ttcagtagca cgcttctttg aagctgcagg cgagagatga
    20341 taaaggcggc taaacatcgt taagttatcc cagccagtta acgtttcatc aactgcggca
    20401 tattgacctg ataatccaat tacctcacgt actttgcctg gatctttaat tacatcaatg
    20461 ccagcgacgc tcgcatttcc tgaatctgga gttaaaagcg ttgccaaaat tcgaaccgta
    20521 gttgtttttc cagcaccatt tggaccaaga actccaagaa ctgttccctc ttcgacatca
    20581 aggctcaagc catcaagagc tcttactttg cccttgtcgt aggttttgat taggttttct
    20641 gcaataactg ccttcacctg cccgatagta gtaatcttcg ctctctattg ccaattgcgc
    20701 atcgctagaa aggcttgatt taaaattaag ttcaattcac ccttcaagga cctgcctaaa
    20761 gaagtagcgg tattaacctc aatatctttt ctagtggctg ttggctttgg gctcataatt
    20821 cctgccattc caatctttgc taaatcattt ggtgtttcaa acactttaat tggatttatc
    20881 atctctgcct ttgccattat gagatttatc tcggggctat ttgcaggaaa attagtagac
    20941 cgatttgggg agcgattagt acttggcgta ggccttttta tggtctcggt ttttactcta
    21001 atttctgggc ttgctcaaaa ctatgaacag ttattgatat ttagaactgc aggcggcctt
    21061 ggatcatcaa tgttctcagt ttcagctggg gcgttaattc ttagagttgt ttcagatagt
    21121 caaagaggtc aggctcaatc actttataac ggtggtttca tcgccggagg agttgcaggc
    21181 ccagcatttg gtggagcatt aattgcgctc tctccaagag cgccgttttt catctactcc
    21241 ggtcttttaa ttgtggcagg aactgtttca ttaatatttc ttcacgaaag tagattgggc
    21301 gcaaaagttg agcaaagtaa agaggagcgc gcactaacaa ttaaagaagc tctggcaatc
    21361 aagccttatc tctactcact atttctagca tttcttggaa gctggattct ctttggtatg
    21421 cgctcatcta tcttgccgct ttttgttgtg gaagatctca aagcaagtcc atctcttgtg
    21481 ggcttgagtt ttacagtagc gctaatcgct cagggttcgg tgatggttcg agctggaaag
    21541 cactcagata agaacggacg aaaaccagtc atccttactg gattttctat cgtaatggca
    21601 tctcttggaa ttcttttcct tgcaaataat gtgactgtct atctaattgc aatgattgtt
    21661 atgggccttg gcgcaggctt tgctgcatct gctggcgcaa ttgttggcga tgtaat
//
LOCUS       DAOROD010000018        19589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_94_length_19589_cov_28.942050, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000018 DAOROD010000000
VERSION     DAOROD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 19589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19589
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94_length_19589_cov_28.942050"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1015
                     /locus_tag="PLW53_04535"
     CDS             59..1015
                     /locus_tag="PLW53_04535"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRD36722.1"
                     /translation="MDSNSEKNALALQVIPRGWSAKDIGDQSGKHFLITGATSGIGLE
                     SARELIRAGAQVTIAARDLKKAEQVVKELSSQRAQILHLDLADLNSVRKAAREVKQDI
                     DVLILNAGVMATPYTKTADDFELQMGVNHLGHFALAGLLADRIKSRVVSVSSSAHRFG
                     NLGEGSQDAIRDICLGRNKYQPWSAYGASKLANLLFTFELERIARKANYSFSAFAAHP
                     GYANTNLQSVGPKMRGSIFEERGAAFANSLIAQSASRGALPTLCAATFPNLYGASYLG
                     PDGLLEMRGFPKATRARSIAYDQGLARNLWSVSSELTGVNWR"
     gene            1025..3484
                     /gene="leuS"
                     /locus_tag="PLW53_04540"
     CDS             1025..3484
                     /gene="leuS"
                     /locus_tag="PLW53_04540"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRD36723.1"
                     /translation="MLSAYDFHFIQEKWLPIWDKLAPFRSGDASDPRPKKYVLDMFPY
                     PSGDLHMGHAEAYALGDVIARYWVQKGFNVMHPIGWDAFGLPAENAAIKRNEDPSIWT
                     YENINTQKASMRRYACSFDWDRVLYTCDSEYYRWNQWIFLKLYEKGLAYRKNSPVNWC
                     PDCQTVLANEQVIAGLCERCDNPVTKKKLSQWYFKITDYAERLLDDMKELEGKWPEKV
                     LTMQRNWIGKSVGAEVKFNIEGREEALTVYTTRPDTLYGATFMVVAVDSELARELVAN
                     TEVQADFEKYFDSVKSASDIDRLATDRAKSGIFLGRYAINPVNNERIPIWASDYVLAD
                     YGTGAIMAVPAHDQRDLDFARAMKLPVRVVVQVEGQEDPAVSGVATSGSGVMVNSGSL
                     NGLESAAAIGKIIAELEAKNLAKASNNYRLRDWLISRQRYWGTPFPIIHCQKCGEVPV
                     NESDLPIKLPDSKSLDLRPKGTSPLATATDWVNVKCPKCGAGALRDTDTMDTFVDSSW
                     YFLRYTSVNTHDKPFDRKEVDTWLPVDQYVGGVTHAILHLLYSRFFTKALKDMGYVDF
                     SEPFTRLLNQGMVLMDGSAMSKSRGNLVALSEELEKHGVDAIRLSMVFAGPPEDDVDW
                     ADVSPTGSSKFLHRAWRLAGDVKSEPGIDFSKGDLELRKVSHKALSDISFAVESFRFN
                     VVVARIMELVNAARKAIDSGVGPSDAAVREAVEIVAISLSLIAPYTAEEMWEVLGHQP
                     SVARAGWPSVDPQLLTQDSVTAIFQINGKIKSRVEVSPDIADEALEKMALSDPAIVSD
                     LGGVSPKKVITKAPKFVNIVL"
     gene            3565..4164
                     /locus_tag="PLW53_04545"
     CDS             3565..4164
                     /locus_tag="PLW53_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRD36724.1"
                     /translation="MDFAKLKEDFQDYTYSQRRALIAVSLVAILFATFLFTNTRGRAV
                     AQESAKPTLTMPAVAKTILIHVAGKVKRPDVYPLLAGSRVSDAIKAAGGANKGVDLGD
                     INLARVLIDGEQVYVGYVPKASSSSSKSSKSRFTGVININRATKAEFDSLAGIGPVIA
                     SRIINYRNTNGPFLALDDLLKVSGIGSKTLERIRPRLSL"
     gene            4227..6440
                     /locus_tag="PLW53_04550"
     CDS             4227..6440
                     /locus_tag="PLW53_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRD36725.1"
                     /translation="MGATYFSWSALAVSAVITSFTYLFKRRIYIISLALLIGSILFSI
                     HSQILQSSVIAQLAKSSSDVKVIASVTSDPKLTFERVRGSDINPSQSSFLARASYVES
                     EEASYEVRVAIRVLIPKKVELNPGDLVQFQGRLIKSKERRVAALLIADEEFTQLSKVG
                     ALGQLLAETRSKFRNQAAVYESNSGALIPGMILGDTSLQSEEFTKQMRRSGLAHLTAV
                     SGANFAIVSALVFFILRLVISNLGVRIVTTGLILFLFLLLVRPSPSVLRAGLMALVVL
                     LAIATGNRANAAAALSSAVIVLLLFDPFQGQDPGFILSVLATAGLIFLSPGIKAYLLR
                     FLPSWLAEIIAVSTAATLLCTPYLIAFTSQAPLLAIPFNILTSLLVAPVTVLGFLSVL
                     TLPIAFISEGLLAIAEFLARWIAYIASLSDLTPTLSLSHQLLLITLALVIAVLFFAPL
                     RRIAIAALVALTFFGIFSKASFPGDAWRIVQCDVGQGDALVLKISADSALLFDAGPDP
                     DLLDRCLKSIGIKHLPLIVLSHNHADHFYGVSGALRKREIGQIWSNGNISRAELSNYE
                     VSKVGAGDIARIDDVSLEVLWPPAATSNFSNLSGDGSQENNRSLVILAQVGQVKILIT
                     GDIEPEAQEEIANRYDLAGISILKVPHHGSRFQSELFLRQISPEIAIISVGESNSYGH
                     PDSSLVEKLRVMGSRVYRSDKDGPISLSWRFDEGAMGYIFTTRNVGRKWWQIQWL"
     gene            6419..7387
                     /gene="holA"
                     /locus_tag="PLW53_04555"
     CDS             6419..7387
                     /gene="holA"
                     /locus_tag="PLW53_04555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRD36726.1"
                     /translation="MADSMALANLLLIHGAESVLADRALADALVARGDYEKTVLEGTE
                     LEIGGFASAIAPSLFADKRVVVLKDLQDLISEVQEEVENYLSALDSSLHIIFIHKGGV
                     KGKALLEKIKKLKPEIILCEAMKKESDKASFVAQEFERRGRKISSAAISALVDATGSD
                     TRELAAACSQIAFDTSAAKAIIDEEDVALYYQGRVQATGFDVADATLAGDSKAALISL
                     RNALDTGVDPIMILSAITSSIRALAKVSGAPRGANAFQLAGSLGLAPWQIDKARRQLG
                     KWTPALIAFSVGELAKADVAIKGAEADPIYALERSVLAIAGKVGRK"
     gene            7487..7756
                     /gene="rpsT"
                     /locus_tag="PLW53_04560"
     CDS             7487..7756
                     /gene="rpsT"
                     /locus_tag="PLW53_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRD36727.1"
                     /translation="MANIKSQIKRIKTNEKARLRNKSVRSSIKTAIRRFRDSLTAGDS
                     KLVATELSNASRELDIAVSKGVIHANQAANKKSAMAKSAHKAGVR"
     gene            7793..9661
                     /gene="lepA"
                     /locus_tag="PLW53_04565"
     CDS             7793..9661
                     /gene="lepA"
                     /locus_tag="PLW53_04565"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRD36728.1"
                     /translation="MPAIDLSKAPQPAATNPEQIRNFCIIAHIDHGKSTLADRMLGIT
                     GVVEARNMRAQYLDRMDIERERGITIKSQAVRLPWKSALDGKEYILNMIDTPGHVDFT
                     YEVSRSLAACEGAILLVDCAQGIEAQTLANLYLAMENNLTVIPVLNKIDLPSAQPDKF
                     AIELSKLVGCKPEDCLRVSGKSGIGVEELLDQIIAQIPAPKGNPQGPARALIFDSVYD
                     TYRGVVTYVRVFDGHLSPREQIQMFSTGVKHELLDIGVISPEPVSSKGLGVGEVGYLI
                     TGVKDVRQSRVGDTVTTFHKPALEALSGYKDPKPMVFSGLFPIDGDQYPLLRDALDKL
                     QLNDAALVYEPENSAALGFGFRCGFLGLLHMEIVRERLEREFDLTLISTAPNVVYTVT
                     TEAGQELIVTNPSEYPNGKVAQVREPIVKATILSPTDYIGTIMELCQQRRGALNGMDY
                     LSEDRVEIRYTLPLAEIVFDFFDQLKSRTKGYASLDYEPIGEAEGDLVKVDILLQGET
                     VDAFSSIVHREKAYTYGVMMTSKLRELIPRQQFEVPIQAAIGAKIIARETIRAIRKDV
                     LAKCYGGDISRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSTNADEESGKVKK"
     gene            9663..10808
                     /gene="hemW"
                     /locus_tag="PLW53_04570"
     CDS             9663..10808
                     /gene="hemW"
                     /locus_tag="PLW53_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRD36729.1"
                     /translation="MSQRLSFYIHIPYCAKRCGYCDFNTYTPSELKSGDLDSLSSSYI
                     DSALKEIEMAAQAAGPAIIPTIFFGGGTPSLLPAAQLSRIIDAIKSRFTLDKNIEITL
                     EVNPDSVTQEFLDEIKSAGATRISMGMQSAVGSVLKVLDRTHNPENVERAVSMARAAG
                     FEHVSVDLIYGSPGESMDDWRRSVEYALALDIDHISAYALIVEKGTKLAAQINRGELT
                     MPPDDQSADKYLLADQLFEAAGFNWYELSNWSKPGGQCRHNIAYWDGSFWWGVGAGAH
                     SYLNGKRWWNVKHPSSYQEKILQGQSPELSHEILTAENLSDEFIMLQIRRREGILHNH
                     LSSAQIAKAEEFLNSGFLDSASWQDMRLVLSRDGRLIADKIVRELVL"
     gene            10821..11165
                     /locus_tag="PLW53_04575"
     CDS             10821..11165
                     /locus_tag="PLW53_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD36730.1"
                     /translation="MATMHTVITLLLAVIFLIAGLSKASGSSAGLSGTRDVGFPDGLA
                     RLVGIFETLASTSLVIGFALENSDLELYGYIVIWFVMAGALFFHFRANKIRTGFPAML
                     LIVLATLGIATL"
     gene            11192..12205
                     /gene="hrcA"
                     /locus_tag="PLW53_04580"
     CDS             11192..12205
                     /gene="hrcA"
                     /locus_tag="PLW53_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRD36731.1"
                     /translation="MESRQLEILKAIVEEYVATEEPVGSKIISEKSALGISSATIRNE
                     MSILEGQGLITQPHTSAGRIPTDKGYRLFVDKIATVKPLSSAERRAIETFLEGANDLD
                     DIVMRTVRLLAQVTKQVAVVQYPLLTKSRVRHIELVLLTPSRLMLVLITTTGRVEQRV
                     LELPYDLSDALLADLRQRLNAAVVGKAMSDVGQDLEAILAHFGKNERTSTALIISNLI
                     EMAIERPEEKVVLAGTANLARSNSESSAEIHGVLEALEEQVVLLRLLSDAGDTMRVRI
                     GAEQSESGLQRTSLVSMGYGVDGNSLGALGILGPTRMDYSSSMSAVNAVARYVGRFLM
                     DNQ"
     gene            12205..13329
                     /gene="dnaJ"
                     /locus_tag="PLW53_04585"
     CDS             12205..13329
                     /gene="dnaJ"
                     /locus_tag="PLW53_04585"
                     /inference="COORDINATES: protein motif:HMM:NF010871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRD36732.1"
                     /translation="MSDLYKALGVERNASQDEIKKSYRKLARELHPDVNPDPKVQDRF
                     KEITAAYEILSDPEKRSNYDRGGDNFAGFGGGFGGFSDIMDAFFGGGGNPRGPRARTR
                     QGQDALIRTTITLAEACFGTDREIGVETAIICTKCGGDGCAPGTSPSTCDICRGRGEI
                     QQVTRSFIGQVMTSRPCGTCQGFGTVIPQVCTECSGDGRVRARRNVSVKIPAGVETGN
                     RMQLSGQGEVGAGGGPAGDLYVEMIVEEHEYLLRDGDNLHIAVSVPMTSAALGSEMNI
                     ETLDGEIDIEIKPGTQSGDVIAVKGKGMTKLRGGGRGDLYAHVSVEVPSKLSKKESDL
                     LKEFAKLRSEDKSSNKMKSNKEDGQGSSGFFGKFKEAFRN"
     gene            13329..14063
                     /locus_tag="PLW53_04590"
     CDS             13329..14063
                     /locus_tag="PLW53_04590"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRD36733.1"
                     /translation="MLPVFIVDELPDKGELFVSGDEAHHAINVTRIKVNEIIALTDGK
                     GRKVSVEVTSITKKSFSAKVLTVQDFPASKIWLTVMQALTKGDRARETIELLTEAGVD
                     EIIPWSANRSIGQFKDEQDSLDKWRSWARESTKQSRRAWFPKISTLQSGVSASEVLMD
                     FDLTLLFHESDGGKLSDLLAKAQSEKNLNKVLLIIGPEGGISDEEAAAFLERGALSVA
                     MGLPIFRSAHAGAAALAAVQTGLGIW"
     gene            14073..14408
                     /locus_tag="PLW53_04595"
     CDS             14073..14408
                     /locus_tag="PLW53_04595"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRD36734.1"
                     /translation="MKCLFCAIVNGEIPADIVRQSDNVLAFNDINPVAPTHVLIIPKI
                     HVENAAELTLANPEVLIECFGMATSIAKERELNGYRTVFNTGADGGQSVFHAHLHLLG
                     GRALSWPPG"
     gene            14419..15426
                     /locus_tag="PLW53_04600"
     CDS             14419..15426
                     /locus_tag="PLW53_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD36735.1"
                     /translation="MDRITLTLPSDISSVSITGPNDSYIKAIESAFHDVAITVRGNEV
                     ILRGSTQGCERVKQLIQSFLTVIRTGQPLSDDAVRRSIAMINDGSSPAEVLTLNILSN
                     RGKTIRPKTSNQKRYVDAIDKNTVTFGIGPAGTGKTYLAMAKAVQSLQSKQVNRIILT
                     RPAVEAGERLGFLPGTLHEKIDPYLRPLFDALHDMLDPEAIPRLMSSGTIEVAPLAYM
                     RGRTLNDSFIILDEAQNTSPEQMKMFLTRLGFSSKMVITGDVTQIDLPSHQDSGLKIV
                     RDILEGIDDISFMDLTSEDVVRHRLVSEIVDAYGRFDNLVAGNRADRRENKPRSLRSD
                     R"
     gene            15457..15903
                     /gene="ybeY"
                     /locus_tag="PLW53_04605"
     CDS             15457..15903
                     /gene="ybeY"
                     /locus_tag="PLW53_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRD36736.1"
                     /translation="MNIELSNLTDSKCDLERLKSIAQFTILAQGVHPDSELNISLLDE
                     DEMSALHLRWMEEDGPTDVLAFPMDEVKPNSATLGPAMLGDIALCPTYANQNALKVHS
                     SLQDELELLTVHGVLHLLGYDHMEADEKEVMFALQDKYLVEWRAKR"
     gene            15900..17189
                     /locus_tag="PLW53_04610"
     CDS             15900..17189
                     /locus_tag="PLW53_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRD36737.1"
                     /translation="MSLTEVLSVVALLLFAGFLAGSESAINSISRVFVEELEVKSSKS
                     AAWVARVIKEPARYLNVILFVRKASELTATVIVAQAFIVLFEPIALAMFIAVAAMVVL
                     SYVVVGVGPRTLAKQNAGRWIIPACVVAVVMSKILGPLTALLIAIGNAITPGKGFKSG
                     PFANEAELRDLVDQAHERGLVEESEHEMIHSVFELGDTLVRELMVPRTEMVWIEGGKN
                     LRQGLSLALRSGFTRIPVIGENLDEILGIAYVKDLARRTHEYREAELTELVSQHIRGA
                     TFVPETKTASELLKEMQRDQIHLAIVVDEYGGTAGLITIEDILEEIVGEIADEYDDDV
                     EEFEWVTTSQVRVSARLHLEDLADKLEFELSEDEKEDVDTIGGFVAKSLGRVPIPGST
                     VTLKGWVLTAERPIGRRHRIATFLVERPADLVPDKEV"
     gene            17199..17987
                     /locus_tag="PLW53_04615"
     CDS             17199..17987
                     /locus_tag="PLW53_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD36738.1"
                     /translation="MRIVRFSPPAGSKLGSDPHFGLLEDDMSITAIASDPLYAGIFKQ
                     EEVFALSEVKLLAPVIPRSKVVCVGKNYADHAAEMGGEVPVEPIIFIKPNTSVIGPED
                     LIQWPDTSERIDHEAELAVVISKICKEVPREKVNDVIFGYTLANDVTARDLQKKDGQW
                     SRAKGFDTFCPLGPWIDTNFVPGDQLITAHVDGELKQSAKLSDMVFDVPFIIEFVTKV
                     MTLLPGDVILTGTPSGIGPMNPSGEVSVSIEGLGTLRNRVSSRG"
     gene            17980..19446
                     /gene="gltX"
                     /locus_tag="PLW53_04620"
     CDS             17980..19446
                     /gene="gltX"
                     /locus_tag="PLW53_04620"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRD36739.1"
                     /translation="MANSSSREVRTRFAPSPTGDLHVGNIRTALFAWAYARHCSGKLI
                     FRVEDTDRERVTDEYIARAIETLKWLGINWDEGPEVGGEHGPYLQSQRLDVYKHWADI
                     FIKNGDAYHCYCSTEELEKRRDKQRELNQAPGYDGKCRDLTSSEIEAFIKEGRAPVVR
                     MRMPDGSTTFNDLIRGEVSFEHKYVPDFVLMRADGSPLYTLAVAVDDVLMKITHVLRG
                     EDLLSSTPRQIRVYQAMGVKKEEFPVFAHLPFVMGQDNAKLSKRNGEVSIAWYRESGF
                     LPEAICNYLALLGWSPGDDREDVSMKELVELFELERVNSSPARFDMKKLEAINGEKIR
                     KLTLDDFLSRALPFLLKDGVVSGKDDELKVLRQALPIIQERIARMNEVSAMLKFLFTE
                     QLSFDQEASAALLGDQAQAVIAKSLEVLQGVDDWSHGAIEAKLREALIDQMALKPRLA
                     FSPVRIAVTGSHISPPLFESMELLGKQRCITRLKAAVS"
BASE COUNT         5548 a         3873 c         4746 g         5422 t
ORIGIN      
        1 tagcgcacct gcatggcatg cagggggtca ggggttcaag tccccttagc tccacgttat
       61 ggatagcaac tcagagaaaa acgcgctcgc tcttcaagta attccgcgcg gttggagcgc
      121 aaaggatatt ggcgatcaaa gcggcaaaca ttttcttatc accggagcaa cgagcggcat
      181 tggtcttgag agcgctagag aactaattag agcaggggcg caggtaacaa ttgctgcaag
      241 agatctaaag aaagccgagc aggtagtaaa agagttatca agccagagag ctcaaattct
      301 ccatctagat ttagccgatt taaatagcgt tagaaaagca gcgagagaag taaagcaaga
      361 cattgatgtt ttgattttaa atgctggggt catggctact ccatatacaa agactgctga
      421 tgattttgaa ctacaaatgg gagttaatca cctaggccac tttgcacttg ctggactgtt
      481 agcagatcga atcaaatcaa gagttgtctc ggtatcaagt tcggcccatc gatttggaaa
      541 tttgggtgaa ggtagccaag atgcaattag ggatatctgt cttggcagaa ataaatatca
      601 accctggtcg gcatatgggg caagtaaatt agctaatctg ctatttacat ttgaattaga
      661 gagaattgct agaaaagcta attatagttt tagcgcattt gccgcccatc ctggttatgc
      721 aaatactaat ttgcagagtg ttggaccaaa gatgcgtgga agtatttttg aagagcgagg
      781 ggcagccttt gcaaactccc ttattgcgca atcagcatca agaggagcgc ttccaacgct
      841 atgcgctgca acatttccaa atctctatgg cgcttcatat cttggtcctg atggattgtt
      901 ggagatgcgc ggttttccaa aagcaacaag agctcgctct attgcctatg atcaaggctt
      961 ggctcgaaac ctctggagcg ttagcagtga attaactgga gttaattggc ggtaatctag
     1021 ggcaatgctc tcggcctatg actttcactt tattcaagag aagtggttgc caatttggga
     1081 caagctagcg ccatttagat ccggggatgc aagtgatccc cgccctaaga aatatgtact
     1141 ggacatgttt ccctatccat cgggcgatct tcatatgggc catgccgagg catatgcttt
     1201 aggtgatgtg attgctcgtt actgggtgca aaaaggcttt aacgttatgc atccaattgg
     1261 ctgggatgct tttggtctcc cagcagaaaa tgctgcaatc aagcgcaatg aagatccaag
     1321 tatttggact tatgaaaata taaataccca gaaagcttca atgcgaaggt atgcctgctc
     1381 atttgattgg gatcgagtgc tttatacctg cgatagcgag tattaccggt ggaatcaatg
     1441 gatattttta aaactatatg aaaagggatt ggcttatcgc aagaactcac cagttaattg
     1501 gtgcccagat tgccagactg ttttggcaaa tgagcaggta atagcagggc tctgtgaacg
     1561 ctgtgataat ccagtaacaa agaagaagtt gagtcagtgg tatttcaaaa tcactgatta
     1621 tgccgagaga ttacttgatg acatgaaaga acttgagggc aagtggcctg agaaagttct
     1681 aactatgcag cgcaattgga ttggaaaatc cgttggagct gaagttaaat tcaatatcga
     1741 aggaagagaa gaagccttaa cggtctatac caccaggccc gatactctct atggcgcaac
     1801 atttatggta gttgccgttg atagcgaact agctagagag ttagtggcaa atacagaagt
     1861 gcaagctgac tttgagaaat acttcgatag cgttaagtcg gcaagtgata tcgatagatt
     1921 ggcaacagat agagccaaga gcggcatttt tctaggacgc tatgcaataa atccagttaa
     1981 taacgagaga attccaatct gggcaagtga ttatgtatta gctgattatg gaacaggggc
     2041 catcatggct gttcctgctc atgaccagag agatttagat tttgctagag cgatgaagct
     2101 accggtaaga gtggtagttc aagtagaggg gcaagaagat cctgccgtta gcggtgttgc
     2161 tactagtggc tctggggtaa tggttaactc tggcagctta aatgggcttg aaagcgcagc
     2221 agcaattgga aagataattg cagaacttga ggcaaagaat ttagcaaagg catctaataa
     2281 ctatcgcctt agagattggt tgatttcacg tcagcgttat tggggaactc catttccaat
     2341 tattcactgt cagaagtgcg gcgaagtgcc agtaaatgaa agtgatcttc caattaaatt
     2401 gccggattcc aagtcgctag atttaaggcc aaaggggaca tcacctcttg ccacagcaac
     2461 tgattgggtc aatgttaaat gtcctaagtg tggcgcaggc gctttaagag ataccgacac
     2521 catggatacc ttcgttgact catcttggta tttcttgcgc tataccagcg ttaacaccca
     2581 cgataaacct tttgatagaa aagaagtcga tacctggcta ccagtagatc aatatgtagg
     2641 tggagtaact catgccatct tgcatctgct ctattcacgc ttttttacca aagcgctaaa
     2701 agatatgggc tatgtagatt tctcagagcc atttaccaga cttcttaatc aaggaatggt
     2761 tttaatggat ggctctgcta tgagtaagag ccgtggcaat ttagtagccc tctctgagga
     2821 acttgaaaaa catggcgtag atgcaatccg gttatcaatg gtctttgcag gcccaccaga
     2881 agatgatgtt gattgggctg atgtttcacc aacagggtca tcgaagttct tgcatcgcgc
     2941 ttggcgcctt gctggagatg tcaagagtga gccggggatt gatttttcaa aaggtgattt
     3001 agagttaaga aaagtttctc ataaagctct atctgacatc tcatttgcag ttgagtcttt
     3061 tagattcaat gttgtggtag caaggattat ggagctagta aatgccgcaa gaaaagcaat
     3121 tgattccggt gttggcccat cagatgctgc agtaagagaa gccgttgaaa tagtggcgat
     3181 atcactttct ttaattgcgc cctataccgc tgaggagatg tgggaggtat taggccatca
     3241 gccatcagtt gcaagagcag gatggccaag cgttgaccct caactattaa ctcaagactc
     3301 agtcactgct attttccaga taaatggcaa aatcaaatct agggttgaag tatcaccaga
     3361 tattgccgat gaagcccttg agaagatggc tttaagcgat ccagcaatag tctctgatct
     3421 tgggggagta agccctaaga aggtaattac caaggccccg aagtttgtaa atatcgtttt
     3481 atagcaatat ccacaacgcc aacttctcca ctaaaagagt tagtcaaaga aataaataat
     3541 tgcaatacct gatttcatca ggccatggat tttgcgaaat tgaaagaaga ttttcaagat
     3601 tacacctact cacagcgaag agcgcttatt gctgtatctc tagttgccat tctctttgct
     3661 acgttcttat ttacaaatac taggggacga gcagttgcgc aagagtctgc taaacccacc
     3721 ctaactatgc cggcagtagc caagacgata ctcattcatg tcgctggcaa agttaaacga
     3781 ccagatgtgt atccactact tgcaggctct agagtctcag atgcaataaa ggcagcaggt
     3841 ggagcaaata aaggcgttga tttaggtgac atcaacttag ctcgagtctt aatagatggc
     3901 gaacaggtct atgtgggata tgttccaaaa gctagctcaa gcagtagcaa gagtagtaag
     3961 tctagattta ccggagttat caatatcaat cgggctacga aggctgagtt tgattcactt
     4021 gctggaatcg gaccggtgat tgctagccga ataattaatt atcgaaatac aaatgggcca
     4081 tttcttgccc tagatgatct actgaaagtt tctggtatag gtagcaaaac tctggagcgg
     4141 attcgcccac gtttgagcct ctagaccaga gggtaagaag tgattaccgc gcactgctct
     4201 ttgctctagc cctctgggtt ggggtactgg gtgcgaccta tttctcttgg tctgcgttag
     4261 cggtaagtgc ggtaatcaca tcttttacct atttattcaa gcgacgtatt tacatcatct
     4321 cactcgcgct ccttatcggc agcatcctat tttcaattca tagtcagatc ttgcaaagta
     4381 gcgtaattgc tcagttagcc aagagctcta gcgatgtgaa ggtgatagca agtgttacta
     4441 gtgatccaaa actaacattt gaacgagtcc gtggctcaga tataaatcct tcgcagagtt
     4501 catttctagc tagagcctct tatgtagaaa gtgaggaggc tagttatgaa gtaagagttg
     4561 ctattagagt cttaatccca aagaaagtgg agttgaatcc tggtgattta gttcaattcc
     4621 aaggaagact gataaagagt aaagagcggc gagtggctgc tctcttgatt gcagatgaag
     4681 aattcactca actatccaag gtaggagctc tgggtcagtt actggcagag actcgatcta
     4741 aatttcgcaa tcaagcagcg gtatatgaaa gtaattctgg cgcgctaatt cctggaatga
     4801 tcttggggga tacctcactt caaagcgaag aatttactaa gcagatgcgc cgatctggcc
     4861 ttgctcatct cacagcagtt agtggcgcaa attttgccat tgttagcgcg ctagttttct
     4921 ttattctgcg actagttatt tcaaatcttg gcgtaaggat agttactacg ggattaattc
     4981 tctttctttt cctactttta gttaggccat cgccctcagt tttacgggca gggctaatgg
     5041 ctcttgtagt tttattggca attgccactg gcaatcgagc aaatgctgca gctgccttat
     5101 ctagcgcagt aattgttctt cttctctttg atccctttca gggacaagat cctggcttta
     5161 tcctctctgt tcttgctact gctggtttga tatttctctc acctggaatt aaggcatacc
     5221 tacttcgatt tctaccatct tggctggctg aaattattgc ggtttcaact gctgcaaccc
     5281 tgctctgtac gccttatttg attgccttta cctcccaagc tccacttctt gctattccat
     5341 ttaatatctt gacctcactt ttagtagctc cggtgaccgt tcttggtttt ctttcagtgc
     5401 tgactcttcc tatcgccttc atatcagagg ggctattggc tattgcagag tttcttgctc
     5461 gttggattgc ctacattgca tcactctctg atctaacgcc aacgctttcg ctctcacatc
     5521 aacttcttct gataactctg gctctagtta ttgcagtttt attctttgcc cctttgagaa
     5581 gaattgcaat cgcagcacta gttgcactta ccttttttgg aatatttagt aaagctagct
     5641 ttccaggaga tgcgtggcgc atagtgcaat gcgatgttgg tcagggtgat gctctggtgc
     5701 taaagatttc tgctgatagc gctctgctat ttgatgctgg ccctgaccct gatctacttg
     5761 atcgttgttt aaagagcatt ggtatcaagc atttgccact aattgtctta agccataatc
     5821 atgctgatca tttctatggg gtctccggag ctttaagaaa gcgagagatt ggacagatct
     5881 ggagtaatgg gaatatctct agagcggagc tctctaacta tgaagtatca aaggttggag
     5941 ctggcgatat tgcaagaatt gatgatgtat cgctagaggt tctttggcca cctgcagcta
     6001 cctctaattt ttcaaatcta tctggcgatg gatctcagga aaataatcgc tccttagtaa
     6061 ttcttgccca agtggggcag gtaaagatat taattactgg tgatattgaa cctgaagcac
     6121 aagaggagat tgcaaataga tatgacctag ctggcatttc aatattgaag gttccccatc
     6181 atggctcacg ttttcaaagc gaactcttct tacggcaaat ctcgccagag atcgcaatta
     6241 ttagcgtggg tgaatccaat agttatggcc atcccgattc ttcccttgtg gagaagctaa
     6301 gggtcatggg ctctagggtt tatcgaagcg ataaggatgg gccaatttca ctgtcctgga
     6361 ggtttgatga gggggcgatg ggctatatat tcactactag gaatgtagga aggaagtggt
     6421 ggcagattca atggctttag caaacctctt acttatccat ggcgcagaga gcgttttagc
     6481 agatcgcgcc ctagccgatg cgctagttgc gcgcggtgat tatgagaaaa ctgtcctaga
     6541 aggtactgaa ttagaaatcg gcggctttgc ctcagcaatt gctccttcac tctttgctga
     6601 taaacgcgtt gttgttttaa aagacttaca agatttaatc agcgaggtgc aagaagaagt
     6661 agaaaattac ttatcagcac ttgattcctc gcttcatata atttttattc ataagggcgg
     6721 cgttaaagga aaagcgcttc tggaaaagat taagaagtta aagcccgaga taattctctg
     6781 tgaggcgatg aagaaagaga gtgataaagc aagctttgtc gcccaggaat ttgaaagacg
     6841 aggtcgcaag atttcatcag ctgcaattag cgccctagtt gatgcaacag gaagtgacac
     6901 aagagagcta gcagcggcat gtagtcagat tgcttttgat accagcgctg ccaaagcgat
     6961 aattgatgaa gaagatgttg ctctttatta tcagggaaga gttcaagcca caggctttga
     7021 tgtagcagat gcaactttgg caggagatag caaagctgcg ctgatttcac taagaaatgc
     7081 ccttgatact ggagttgatc caataatgat tctctctgca atcacttcat caattagggc
     7141 gcttgcaaaa gtctcaggag ctccgcgcgg ggccaatgcc tttcaacttg ctggctctct
     7201 tgggcttgcc ccctggcaga ttgataaagc taggcgccag ttaggaaagt ggactccagc
     7261 gctaattgca ttctcggttg gtgaattagc aaaagctgat gtggccatta aaggagctga
     7321 ggccgacccg atctatgccc tggagcgaag cgttctggca attgccggca aagtagggcg
     7381 taaataggcg gtatttggca aggctgagaa tctcggtttg agtcgagata actctggctg
     7441 ataccctgcc gcgttgtaat tcactcaaat ggaaggcttt aagttcgtgg caaatatcaa
     7501 gtcccagatc aagagaatca agaccaatga gaaggctcgc ctgcgtaaca agtcagtgcg
     7561 ctcctcgatt aagaccgcta tccgtcgctt ccgtgattcc cttactgcag gagattcaaa
     7621 gcttgttgcc actgagctat caaatgcctc tcgtgaactc gatatcgcgg tatcaaaagg
     7681 cgtaatacat gccaaccaag ctgctaataa gaagtcagct atggctaagt cagcccacaa
     7741 agctggcgtt cgttaatcca gccaactctt tcaaacaaag taggcgatag gcatgcctgc
     7801 aattgactta agtaaagcgc cgcagcctgc tgcaactaac cctgagcaga ttcgtaattt
     7861 ctgcatcatt gcccatattg atcacggcaa gtccactctg gcagatcgaa tgcttggaat
     7921 aactggagtc gttgaagcaa gaaatatgcg agcccaatat ctagatcgta tggatattga
     7981 acgcgagcgc ggaattacta tcaagagcca agctgttcgt cttccttgga aatcagctct
     8041 tgatggcaag gaatatattc taaatatgat tgatacccca ggacatgttg atttcactta
     8101 cgaagtatca agatctcttg cagcatgcga aggcgctatt ttattagtgg attgtgccca
     8161 aggtattgaa gctcaaactc tggctaatct ttatctagcg atggaaaata atttaaccgt
     8221 tattccagta ttaaataaga ttgatcttcc ctctgctcaa cccgataagt ttgcaattga
     8281 attatcaaag ctagttggat gcaaacctga ggattgcttg cgagtctctg gaaaatcagg
     8341 aattggtgtt gaagaactct tagatcaaat tatcgctcaa attccagctc caaaaggaaa
     8401 tccgcagggt cctgccagag ctttaatctt tgactcggtc tatgacacct atcgaggcgt
     8461 tgtgacatat gttcgagtat ttgatggaca tctctcgcca agagagcaga ttcagatgtt
     8521 ttcaactgga gttaagcatg agctattaga tattggagtt atctcaccag agccagtatc
     8581 aagtaagggc cttggagttg gtgaggtggg ttatctaatc actggcgtaa aagatgttcg
     8641 ccaatcaaga gtcggagata cggtaacaac tttccataaa ccagcacttg aagcgctctc
     8701 tggatataaa gatccaaaac ctatggtttt ctcaggtcta tttcccatcg atggtgatca
     8761 atatccattg cttcgagatg ccctcgataa attacagcta aatgatgcag ctttagtcta
     8821 tgagccagaa aattctgcag cccttggctt tggatttaga tgtggctttc taggacttct
     8881 ccatatggaa attgttagag agcgtctaga gcgcgagttt gatctaacgc tcatctcaac
     8941 tgctccgaat gtggtctaca cagttacaac tgaggccggg caagagttaa ttgttaccaa
     9001 tcccagcgag tatcccaatg gaaaagtagc ccaggtcagg gagccaatag ttaaggcaac
     9061 aattctctct ccaactgatt acatcggaac gattatggag ctttgccagc agcgacgagg
     9121 cgctctaaat ggcatggatt atctctctga agatcgggta gagattcgct acaccttgcc
     9181 ccttgccgaa atcgtctttg atttctttga tcaactaaag tcaagaacta agggttatgc
     9241 ctcactggac tatgaaccaa taggcgaggc tgaaggagat ctagtcaagg ttgatatttt
     9301 gcttcaagga gagactgttg atgcctttag ttcaattgtt caccgtgaga aggcttacac
     9361 ctatggagtc atgatgactt caaaacttag agagctaatt ccaaggcagc aatttgaagt
     9421 tccgattcag gcagctattg gcgccaaaat tattgcccga gagacaattc gcgccattcg
     9481 aaaagatgtt ttggcaaagt gctatggcgg cgatatctct cgtaagcgca aacttttaga
     9541 gaagcagaaa gagggaaaga agcgcatgaa gatggtggga agagttgaag taccacaaga
     9601 ggcattcatc gctgcgcttt caacgaatgc tgatgaagag agcggaaagg tgaagaaata
     9661 gcgtgagcca gcggctctcc ttctatattc acatccctta ctgcgctaaa agatgcggtt
     9721 actgtgattt caatacctat accccgagtg aattaaaaag cggagattta gactcacttt
     9781 catcctccta cattgattca gcgttaaaag agattgagat ggccgctcaa gcagctggcc
     9841 ctgcaattat ccccacgatt ttctttggcg ggggaactcc ttcacttcta ccggcagcgc
     9901 aattatcacg aataatcgat gcaatcaaaa gcagatttac tctagataaa aatattgaga
     9961 taacacttga ggtaaaccca gattcggtca cacaagaatt tctagatgaa atcaagagcg
    10021 ctggagcaac gcgaatttct atgggaatgc aatctgctgt tggctcagta ctaaaagttc
    10081 tagatcgaac tcataatcct gaaaatgttg aacgcgcagt ttcaatggct agagctgctg
    10141 gatttgaaca tgtaagcgtg gatttaatct atggctcacc tggtgaaagc atggatgatt
    10201 ggcgaagaag tgttgagtat gcacttgcgc tagatatcga tcacatcagc gcttatgccc
    10261 taattgttga aaaaggaaca aagttagcgg ctcaaataaa tcgcggcgag ttaacaatgc
    10321 cgccagatga tcagagcgct gataagtatt tgcttgctga tcaactcttt gaagcagcag
    10381 gatttaattg gtatgagtta agtaattgga gtaagcctgg cgggcaatgt cgtcataaca
    10441 tcgcttattg ggatggctcc ttttggtggg gagttggggc aggagctcac tcctatttaa
    10501 atggtaagcg ttggtggaat gtaaaacatc caagttcgta tcaggagaaa atcttgcaag
    10561 gccagagccc agagttaagc catgagatat tgacagcgga gaatctctct gatgaattca
    10621 tcatgctaca aatcagaaga cgtgagggaa ttctgcataa tcacttgagc tctgcacaga
    10681 ttgctaaagc tgaagaattt ctcaactctg gttttctaga tagtgcaagt tggcaagata
    10741 tgcgattggt attaagcaga gatgggcgct tgattgccga taaaatcgtg cgcgagttag
    10801 tcttgtagta actttgccta atggccacta tgcataccgt cattaccctt ctactagccg
    10861 tgattttcct cattgcagga ctatcgaaag caagtggcag cagcgctgga ctttctggaa
    10921 ctagagatgt gggttttcca gatggacttg caaggctggt tggaattttt gaaactctgg
    10981 cctcgacttc ccttgttatt gggtttgcac ttgagaactc agatctagaa ctctatggat
    11041 atattgtcat ctggtttgta atggctggag cgttattctt tcactttaga gctaataaga
    11101 tccgtactgg ttttcctgcg atgctattaa ttgttttggc tactttaggc attgcaacgc
    11161 tttagtgaga atgaaaggga atttctagaa gatggaaagc agacagcttg aaatccttaa
    11221 agccatagtt gaggaatatg tggctacaga ggagcctgtc ggttctaaga taatttcaga
    11281 aaagagcgct ctaggaattt cttcagcgac aattcgaaat gagatgtcaa ttcttgaagg
    11341 gcaaggcttg attactcaac cacataccag cgctggaaga attccaactg ataagggata
    11401 ccggctattt gtagataaaa ttgcaacagt aaaaccgcta tcaagtgcgg aacgaagagc
    11461 catcgaaaca tttcttgaag gcgctaatga tctagatgac attgtgatga gaacagtgcg
    11521 gctacttgcc caagttacaa agcaggtagc agttgttcaa tacccacttc taaccaaatc
    11581 tcgagtaagg catattgagt tggttctact aacaccatct cgattgatgc tggtgctaat
    11641 tactactact ggccgggttg agcagcgagt ccttgagctt ccctatgatc taagtgatgc
    11701 actcttggca gatttgcgac agagattaaa tgctgctgtt gttggcaaag cgatgtcgga
    11761 tgttggtcag gaccttgagg cgatcttggc ccactttggc aaaaatgaga gaacttcaac
    11821 tgctctgata atttcaaacc tcattgagat ggcgattgaa aggcctgagg agaaagttgt
    11881 attagccggc acagcaaatc ttgccagatc aaatagcgag tcatctgctg agattcatgg
    11941 agtattagag gcgctagagg agcaagtagt tttattaaga cttcttagcg atgctggaga
    12001 tacgatgcga gttcgtattg gagctgagca gtcagagagt ggattgcagc gcactagctt
    12061 ggtttcgatg ggttatggag tagatggtaa ttcacttggc gcacttggaa ttcttggccc
    12121 aaccagaatg gattactcat cttcaatgtc tgcagtcaat gcagttgcca gatatgtcgg
    12181 tagatttttg atggataatc agtaatgagt gatctctata aagctttagg cgttgaacga
    12241 aatgcttcac aagatgagat aaagaagagc tatcgtaaat tagcgcggga gcttcacccg
    12301 gatgttaatc cagatccaaa agtgcaagat cgatttaaag aaattactgc tgcctatgaa
    12361 atcttaagtg atccggaaaa acgtagtaat tatgatcgcg gcggcgataa ttttgctggc
    12421 tttggtggcg gcttcggcgg atttagcgac attatggatg ctttcttcgg gggaggcgga
    12481 aatccaagag gtccgagagc aagaactcgc cagggtcaag atgcattaat tagaaccaca
    12541 attaccttgg ctgaagcttg ttttggaact gatcgtgaga ttggagttga aacggcaatc
    12601 atctgcacaa aatgcggtgg cgatggctgc gcgcctggaa cctctccgag cacgtgcgat
    12661 atttgccggg gacgtggcga gattcaacaa gttactagat cttttattgg acaggtaatg
    12721 acatcaaggc cctgtggaac ttgccaaggt tttggcactg taatcccaca ggtctgcact
    12781 gaatgttctg gagatggccg ggtaagggcg cgtagaaacg tttcagtgaa aattccagct
    12841 ggagttgaaa caggaaatcg aatgcagcta tctggtcagg gcgaagttgg cgcaggcggc
    12901 ggacctgcag gagatctata tgttgagatg attgttgaag aacatgaata tctcctccgc
    12961 gatggcgata acctgcatat cgctgtttca gtaccaatga catcagcagc ccttggaagt
    13021 gagatgaata ttgaaaccct agatggtgag atagatattg agattaaacc tggaactcaa
    13081 agtggagatg tgattgcggt caaaggcaaa ggcatgacta agttgcgcgg aggtggcaga
    13141 ggagatctct atgcccatgt aagcgttgaa gttcccagca agttatccaa aaaagagagt
    13201 gatttactaa aggaatttgc caaattgcgt agtgaagata agagttcaaa taagatgaaa
    13261 tcaaataagg aagatggcca gggcagctct ggtttcttcg gaaagttcaa agaggcgttt
    13321 agaaactaat gcttccagta tttatcgtcg atgaactgcc agataaaggc gagttatttg
    13381 tttcaggcga tgaagcccac catgcaatca atgtaacaag gataaaggtt aatgaaatta
    13441 ttgccttaac cgatggaaaa ggccgcaaag tcagcgttga agtaacttcc atcaccaaga
    13501 aatccttttc tgcaaaggta ttaacggtcc aagactttcc cgcctctaaa atttggctaa
    13561 ctgttatgca ggctttaact aaaggcgata gagcgcgaga aacaatagag ttattaactg
    13621 aggcaggagt cgatgagatt attccttgga gcgccaatcg ctccattggt caatttaaag
    13681 atgaacaaga ttcactagat aagtggcgga gttgggcaag agagtccact aagcaatcac
    13741 ggagagcttg gtttccaaag atctcaactc tacaaagtgg agttagcgca agtgaggtat
    13801 taatggattt tgatttaaca cttctctttc atgaatctga tggaggaaag ttgtcggatc
    13861 tgcttgctaa agctcagagt gagaagaatt taaacaaagt cttactaatc attgggcccg
    13921 aaggtggaat tagtgatgaa gaggcagctg cttttttaga gcggggagct ctctcagtag
    13981 caatgggact tccaatcttt cgctctgctc acgccggggc agcagcactt gctgcagtac
    14041 aaactggcct tggaatctgg tagagataac ccatgaaatg cctcttttgc gcaatagtta
    14101 atggtgaaat acctgccgat atagttaggc agagcgataa tgttttagca tttaacgaca
    14161 taaatcctgt tgctccaact catgttttga taattccaaa aatccacgtt gagaatgcag
    14221 ctgagttaac tcttgcaaat ccagaagttc taatcgagtg tttcgggatg gctacaagta
    14281 ttgccaaaga acgagagtta aatggctatc gaaccgtctt taatactgga gctgatggtg
    14341 gccaaagcgt atttcacgct catctgcatc ttcttggtgg tcgtgcactg agctggcccc
    14401 ctggatagaa ttggcgtaat ggatcgaatc actctaacgc tacctagcga tatctccagc
    14461 gtttcaataa ctgggccaaa cgattcctat atcaaagcaa ttgagagcgc atttcatgat
    14521 gtggcaatca cggttagagg aaatgaagta attcttcgtg gaagtaccca aggatgcgag
    14581 cgagtaaagc agctaattca aagttttcta accgttattc gaactggaca gccattaagt
    14641 gatgacgctg ttagacgctc aattgcaatg atcaatgatg gaagctctcc tgctgaagta
    14701 ttgactttaa atatattaag taatcgtggc aaaacaatta ggcctaaaac ctcgaatcaa
    14761 aagcgctatg tcgatgcgat agataaaaat actgtcacct ttggaattgg acctgccgga
    14821 actggtaaga cttatcttgc gatggcaaag gctgttcaat cacttcaatc taaacaggta
    14881 aatagaataa ttcttacccg gcctgcagtt gaggcggggg agagacttgg tttcttgcct
    14941 ggaactcttc atgaaaaaat tgatccatat cttcgcccgc tctttgatgc ccttcatgac
    15001 atgcttgatc cagaagctat tcctcgctta atgtcatcag gaactattga agttgctcct
    15061 cttgcctata tgagaggtag aactcttaac gattcattta tcatcttgga tgaggcccag
    15121 aacacttccc cagagcagat gaaaatgttt ttaaccagac ttggattttc ttcaaagatg
    15181 gttatcaccg gagatgttac ccagatagat ctgccaagcc atcaggactc gggattgaag
    15241 attgtgcgcg atattttaga gggtattgat gatatttctt ttatggattt aacttcagag
    15301 gatgtagtca gacatagatt ggtaagtgaa attgttgatg cctatggacg ttttgataat
    15361 ttagtcgctg gaaatagagc agatagacga gagaataagc cacgttcgct aaggagtgat
    15421 cgctaactta cttaatacca ttaggtaaag tgcgaaatga atatagaact tagtaattta
    15481 actgattcta aatgtgattt agagcgcctt aaatcaattg ctcaattcac tattttggct
    15541 caaggagttc atcctgattc cgagctcaac atctctttgc ttgatgaaga tgagatgagc
    15601 gcgcttcact tgcgctggat ggaggaagat ggaccaactg atgttctagc atttccaatg
    15661 gatgaagtaa aaccaaattc agcaactctt ggccctgcaa tgctgggaga tattgctctc
    15721 tgcccaacat atgccaatca gaatgcgctt aaagttcatt ccagcctcca agatgaatta
    15781 gaattactaa cagttcatgg agttcttcat cttttgggat atgaccacat ggaagcagat
    15841 gagaaagaag tgatgttcgc cttgcaggat aaatacctag ttgaatggag agctaaacgg
    15901 tgagccttac tgaagttcta tcagtagttg ccctcttact ttttgccgga tttttagctg
    15961 gctcagagtc tgcaattaac tccatctctc gagtatttgt cgaagaactt gaggtcaagt
    16021 catctaaatc agctgcttgg gttgcaagag ttataaaaga gccggcccgc tacttaaatg
    16081 tgattttatt tgtaagaaaa gcatcagaat taaccgcaac agtaattgtt gcacaagctt
    16141 ttatcgtttt atttgaacca attgcgctag cgatgtttat cgccgtagct gccatggtgg
    16201 ttctctccta cgtagttgta ggggttggtc cacggacctt ggcaaaacaa aatgctgggc
    16261 gatggattat tccggcctgc gtggttgcag tagtaatgtc aaaaatctta ggcccattaa
    16321 ctgcgctatt aattgcaata ggaaatgcaa taactccagg aaaaggattc aaatctggcc
    16381 catttgctaa tgaggcagag cttcgggatc tagttgacca ggcccatgag cgaggactag
    16441 ttgaagagtc agagcacgag atgatccact cagtctttga attgggcgat actttagtta
    16501 gagaattaat ggttccaaga acagagatgg tctggattga gggaggcaag aacttaagac
    16561 agggtctttc cttagcactt cgctctggat tcaccagaat tccggtgatt ggggagaatc
    16621 tcgatgaaat tcttggcatt gcctatgtaa aagatttagc aagaaggact catgaatatc
    16681 gagaagctga gttaaccgaa ctagttagcc agcacattag aggtgcaact tttgttccag
    16741 aaactaagac cgcatcagag ctattaaagg agatgcagag agaccagatt cacctagcaa
    16801 ttgtggttga tgaatatggc ggaactgccg gtctgattac tatcgaagat attttggaag
    16861 agattgttgg cgagattgct gatgaatacg atgatgatgt agaagagttt gaatgggtga
    16921 ctacttctca agttagagtc tcagctcggc ttcatttaga agacttagca gataagctcg
    16981 aatttgagtt aagtgaagat gagaaagaag atgtcgacac aattggtgga tttgtagcaa
    17041 agagtttggg acgtgtgcca attcctggaa gtacagtcac gcttaagggc tgggttctca
    17101 ctgccgagcg cccaattggc agaagacatc gaatcgcaac atttctagtt gagcgccctg
    17161 ctgatctagt tcctgacaaa gaggtttaga gttagctaat gcgcattgtt agattctctc
    17221 ctcctgctgg ctcaaagcta ggtagcgatc ctcactttgg tctcctagaa gatgatatga
    17281 gcattactgc aatcgctagc gaccctctat atgccgggat ctttaagcaa gaggaagttt
    17341 ttgcacttag cgaagtcaag cttcttgccc cagtaatacc gagaagcaaa gtggtctgtg
    17401 ttggaaaaaa ctatgccgat catgcagccg agatgggcgg ggaagtacct gttgagccaa
    17461 ttatttttat caagccaaat acatcagtaa tcggcccaga ggatttgatt caatggccag
    17521 atacgagtga gcgaatagat catgaagcag agttagcagt ggttatctca aagatttgta
    17581 aggaagtgcc aagagaaaag gttaacgatg tcatctttgg ctacaccctt gccaacgatg
    17641 tcaccgcaag agatctacag aaaaaagatg gtcagtggag tagagcaaaa ggctttgaca
    17701 ctttctgtcc tcttggacca tggattgata ccaattttgt ccctggagat cagttaatta
    17761 ctgctcacgt tgatggcgaa cttaagcaga gtgcaaagct ttcagatatg gtctttgatg
    17821 tgccatttat tatcgagttt gtaactaaag ttatgacgct tcttccagga gatgtgattc
    17881 ttactggaac tccatcagga attggcccga tgaatccatc aggtgaggta agtgtctcta
    17941 ttgaaggact gggaacgcta agaaataggg ttagtagccg tggctaattc atcgtctcgt
    18001 gaggttagaa ctcgttttgc tccatctccc acaggagatc ttcatgtggg aaatattaga
    18061 actgcgctat ttgcatgggc ctatgccaga cattgctccg gaaaattgat tttccgtgtt
    18121 gaagatacag atcgcgagag agtcactgat gaatatattg ctcgcgctat cgaaaccctt
    18181 aagtggctgg gaatcaattg ggatgagggc cctgaagttg ggggagaaca cgggccatat
    18241 ctgcaatcgc aaaggctaga tgtctataag cattgggctg acatctttat caaaaatggc
    18301 gatgcctacc attgctactg ctcaaccgag gagctagaga agagacgcga taagcaacga
    18361 gagttaaatc aagctccagg ttatgacgga aaatgtcgcg atctgacaag tagtgaaatc
    18421 gaagcattta ttaaagaagg tagagctcca gtagtgcgca tgcgaatgcc agatggatca
    18481 actaccttta atgatttaat cagaggtgaa gttagctttg agcataagta cgttccagac
    18541 tttgtcttga tgcgcgctga tggttctccg ctttataccc ttgctgtcgc cgtcgatgat
    18601 gtcttaatga agatcaccca tgtactcagg ggagaggatc tactctcatc aaccccgaga
    18661 cagattcggg tctatcaagc gatgggagtt aagaaagaag agtttccagt atttgctcac
    18721 ctgccatttg ttatgggtca agataatgcc aagttatcta agagaaatgg cgaggtttct
    18781 attgcttggt atcgagagtc aggatttttg cctgaggcaa tctgtaacta cctagctctt
    18841 cttggttggt ccccgggtga tgatcgcgaa gatgtctcga tgaaggaatt agttgagcta
    18901 tttgaattag agcgagttaa tagctcgccc gctagatttg atatgaaaaa gcttgaagca
    18961 ataaatggcg agaagattag aaaattaact ctcgatgatt tcctttcaag agccctacct
    19021 ttccttctta aagatggggt agtcagcggc aaagatgatg agcttaaagt tcttcgccaa
    19081 gctcttccga taattcaaga gcggattgca agaatgaatg aagttagcgc catgcttaaa
    19141 tttttattta cagagcagct ctccttcgat caagaagcta gcgctgctct cttgggtgat
    19201 caggcgcagg ctgtaattgc taaatcttta gaggttctgc agggcgtaga tgattggagc
    19261 catggcgcta ttgaggcgaa attgcgcgag gcgctaattg atcagatggc tcttaagccg
    19321 cgccttgcct tctcgccagt tcgaattgca gtcacaggta gtcatatatc tccaccgctt
    19381 tttgaatcta tggagctact aggaaaacag cgctgcatta cgcgtctaaa agcagcagtt
    19441 tcttaaggcg gaaaattcaa ggtattctta agcccctctt ataagagaag ttgagtaaaa
    19501 atctttgctt aatttttcta gtgagagtaa atgctttggg gtatggggta attggcagcc
    19561 cagctgattc tggttcagct tgtctaggt
//
LOCUS       DAOROD010000019        18990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_101_length_18990_cov_28.905149, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000019 DAOROD010000000
VERSION     DAOROD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_18990_cov_28.905149"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..516
                     /locus_tag="PLW53_04625"
     CDS             169..516
                     /locus_tag="PLW53_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRD36740.1"
                     /translation="MHKIYEMPVADVYVHYVNKVERKDRTEAELIKVITWLTGFDSKT
                     LKNHLKKQTTFKEFFKAAKIHPNAKLITGSICGVKIAEIEDPLMKKIRYMDKLVDELA
                     KGRPMEKILRSGL"
     gene            complement(593..1195)
                     /locus_tag="PLW53_04630"
     CDS             complement(593..1195)
                     /locus_tag="PLW53_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRD36741.1"
                     /translation="MIALKKNPSDRFIAITLLLVAGAMLTVSALQLIPSALDSGLNIM
                     QVLLTTGIGFTVVVLLSSVKFDKDASKVKNPALLITIALSLHNLPEGAAPIGATLVDI
                     QTGLTTSLVLALHNIPEGIAISATALLAGYSRKMALLLTLAATLAEALGAFAVYMSGS
                     LLLDDRSVGLLLSAVAGIMIAICAKELIPYSVKTLHKKQK"
     gene            1352..1801
                     /locus_tag="PLW53_04635"
     CDS             1352..1801
                     /locus_tag="PLW53_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRD36742.1"
                     /translation="MKRVSPPKALRRKSSRESRLVEVINDLDQFLSAQQIHKILNQQG
                     SSIGLATIYRRLERLSQEGKVDALISPSGERLYRRCGQDGGHHHHLICRVCGATQEFD
                     LEAIEVVTSKIAKKHGYQDVSHNLEVFGVCDRCQSSETKSTSKPKLR"
     gene            complement(1756..2286)
                     /locus_tag="PLW53_04640"
     CDS             complement(1756..2286)
                     /locus_tag="PLW53_04640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD36743.1"
                     /translation="MEDLTRGDLANKRPILRLRCNLVDLNLAADNLDSEFELESFIRN
                     KDESRYLNLNNQIFIGHPDQSDWDLSKLLAKMGEVWFRPDRLIFMKKDGKDLGFFWLK
                     EHTLIGRKVCEIYVIGIISQMQGQGLGKKILTCALKQIISDGFSEAWVYTDESNEKAL
                     KLYRSFGFEVDLVSEL"
     gene            2466..3803
                     /locus_tag="PLW53_04645"
     CDS             2466..3803
                     /locus_tag="PLW53_04645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD36744.1"
                     /translation="MSVKPATKEITFKSLGLNPALIKTLDQQGITNPFPIQAATIPDA
                     IAGHDILGRGQTGSGKTLAFGLALLHNIYGMRAKAHQPLALVLAPTRELAQQIDEVLR
                     PLARSVGHECVVIAGGMPYGKQVNALKKSISIVVATPGRLIDLLDRKDIRFDHLEITV
                     LDEADQMADMGFLPAVKEILNQTKKGGQRLLFSATLDRGVDGLVKQYLKEPRSHSIKE
                     IEGTKVKAEHYVFVYRQGDKSNIINQIAARQGKTLLFVRTKRGADKLAANLAHVGVKV
                     GTLHGGKSQAVRTRTLARFKDSESAVLIATDVAARGIHVDGIDLVVHVDLPADHKDYL
                     HRSGRTARAGESGKVISLATHQEQRTMAGIALRAGVETWVMAVTPMDEKLVKIAGART
                     PSGIPTPEAKEKAVTKAPERGSGPRPKGGRKRKRVGNDQTRNKRSAPRAKRAR"
     gene            3830..4282
                     /locus_tag="PLW53_04650"
     CDS             3830..4282
                     /locus_tag="PLW53_04650"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRD36745.1"
                     /translation="MKGKLVVTLQFILLFALALLPGSKDSSQLRSALAALLALLAVLI
                     LFRAFRDLGSALTPLPESKEGADLVTSGIYSKIRHPIYSALFILALAAYLWKQSGSVL
                     IAALLLTSLLLYKARYEDQILRDKFPESIEFQKSIPPFFPRFRKGKKP"
     gene            4279..4776
                     /locus_tag="PLW53_04655"
     CDS             4279..4776
                     /locus_tag="PLW53_04655"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRD36746.1"
                     /translation="MTIGLERLLRHMAWANQLTIEHLATLPEAALKSFATNPEWFVAE
                     ITHHIVDAADAYAFRITGKHPEVSGESIQEIGKIADLEVLAKQAAAIDAALLESAKLD
                     DELLEFKNYSGNLVKRWRSTILSQSIHHATEHRAQIASALEAKGFKPVNLDDLDLWSY
                     EISNG"
     gene            complement(4949..6424)
                     /locus_tag="PLW53_04660"
     CDS             complement(4949..6424)
                     /locus_tag="PLW53_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRD36747.1"
                     /translation="MIERILYVGFDQLNAKYGVLKSADAKKDVIALIQSEPMTTGKNW
                     HPERLYFLISSAKHFAQELREKGFKVEYLKASSTIAGLDELSKKYKDVKIFAAEPSSH
                     RLFQSLSEYGVSFVENDFFLTSRSLFAKWADEQKSYLMENFYRKQRVRLNILMDKAKP
                     LGGAWNFDKDNRLPPPKNYKWPKYLSHAPDEIDLEVAKELGHSPSTNWATTRAGALKQ
                     LDNFLKNHFANFGPYEDAITDENWALHHSLLSPYLNNGLLHPEEVIKAALKRFEKGDI
                     PIASAEAFIRQIIGWREYVNGMYWYLGSEYRQKNGLQAKRELPQLFSDPSKTNMNCMK
                     NTITDINNRAWVHHIPRLMILSNFALIAGINPQQFLQWMREVFIDASDWVMVPNVIGM
                     GVHADGGIMMTKPYAAGGAYISRMSTHCKGCYFDPKKRTGDDACPFTTLYWDFLDRHR
                     QQFQGNHRIAQQLFGLDRLKDLDEVRSRAKDVLKKLDQGKL"
     gene            6461..7528
                     /locus_tag="PLW53_04665"
     CDS             6461..7528
                     /locus_tag="PLW53_04665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD36748.1"
                     /translation="MDLDNLKFGLDTFGDTQNDDSGAPISAAATIRNVVEQAVLADQL
                     GLDHFSIGEHHRDDFAISSPDTVLAGIATRTKQITLGTGVTVLSSDDPVRVYQRFATI
                     DALSNGRAEITAGRGSFIESFPLFGYELSDYGALFEEKLELLVEILKEKAVTWSGTMR
                     APLVNQEIYPKTERGSIPLRVGVGGTPESVVRAARLNAKLAIAIIGGDPARFAPFADL
                     YRSSMNEFGHPIRPISIHSPGHIAASQEIAIEELWPHYQVMFGRIGRERGWGQVTKEH
                     FINEVRYGSFYVGTPEQVARKIAYAMKSIGAERFDFKYSNGPMAHSKLMNSIELYATQ
                     VVPMVKEILSADRAASSASSR"
     gene            7542..8738
                     /gene="nhaA"
                     /locus_tag="PLW53_04670"
     CDS             7542..8738
                     /gene="nhaA"
                     /locus_tag="PLW53_04670"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRD36749.1"
                     /translation="MKRVIAPLKDFLHRESSSGTLILIASALGLLAANSGLADNYFAF
                     LDLKFQIGPSSLELDFTILKSINYLLMSLFFFVVGLEIKRELTSGHLATFKAAAAPLF
                     AAIGGMSLPAIIYLAIAGGVEPNGWAVPVATDIALAVGLLALVGSSAIASLRSFLLAL
                     AVIDDIGAILIIALVYSTGVNLSWGLAGLLTIFAILGFRKLGSQSALVYLPLALSLWY
                     CAYRFGIHPTIAGVILGLLTPNIARTKSNLTDSEDGSVSVIEYLEHKFHPISSYVIVP
                     IFAFANTGVEISSQSINSALNSVIAWGIFLGLVIGKPLGVLLATMAARKIKIAEYPQG
                     ARNLDLIATGSAAGIGFTVAIFIARLAFDSPEIVNLAIFSVIMGSVVSALISLLIFKT
                     LKRSKI"
     gene            complement(8735..9223)
                     /locus_tag="PLW53_04675"
     CDS             complement(8735..9223)
                     /locus_tag="PLW53_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36750.1"
                     /translation="MKLFDGDDLGGPFDLIAGLPLHPLASHAPVVLVPLVAFAALAMS
                     YWPSFSRRYGLPVLILAVISQLSLFLAKASGEAFEERLNKEVERHESYGEIAPFTFIP
                     LLILLFIRYRMDKTGAGIGSPVVRRLVSILLALAAILALVYIFLTGHSGAESVWGWLA
                     KN"
     gene            complement(9245..10834)
                     /gene="ggt"
                     /locus_tag="PLW53_04680"
     CDS             complement(9245..10834)
                     /gene="ggt"
                     /locus_tag="PLW53_04680"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD36751.1"
                     /translation="MSQSTHRSPVYARNGIVAASQPLAVSAGIEILTKGGSAGDAAIA
                     TSAVLAVVEPGASHLGGDAFVISHNAARKKNLAFNGSGEAAHSASADQFKNEIDHHGF
                     KSATVPGLVSTWFEIHKEFGLLSMDQILAPAIDYARNGFAANSGFVKRISGHLKMFPD
                     TQVFKSLGFDTNLKIGDLVIQSDLADSLEAIAQDGRDAFYLGEIARKLVEASDGWFSL
                     RDLAEHRTRVLPPLSVKYRDYLIHGQPPPSQGMILLEELLIAEGFDLAKMQEADRIHL
                     MVEAKKIAFADRYLLLGDPEHIDINLEELFAASSITARRSQIDMERANLKEPSVSSEG
                     SDTTYFLVADRDGNALSWIQSVFHGFGASWVIPGTGILLNNRLTGFSLDPKSPNFIAP
                     GKRPAHTLNAWSVTRDDGSLAYMGGTPGANIQVQSNFQLIVNAIDLKMNPQENAEAPR
                     WQHLNKAGGSSLDEKYEGVLQVENRVEKEILQSLKEKGHSVEELSAYGHGSAVQLLEV
                     LPNGTYIAGSDPRCEGHAAGI"
     gene            10861..11574
                     /locus_tag="PLW53_04685"
     CDS             10861..11574
                     /locus_tag="PLW53_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD36752.1"
                     /translation="MSGVKIEFKNLSKRYKDVLAVSNFSAQVQSGRVTGFLGPNGAGK
                     STALRCLLGLVNPTGGSAAIYGGSYSQLQDPIHKVGAVLDSRGFHPGLTAKQNLKIMA
                     SASDINFSRIDEVLALVDLASAANKRTKGFSLGMKQRLSLAIAMLGDPEVLILDEPAN
                     GLDPLGIVWLREFLQKLAGEGRTILVSSHQLAEMQNTVSDVLIINKGVLVAQGTIKEI
                     CKGKSLEEAFLELTLRKSA"
     gene            11571..12359
                     /locus_tag="PLW53_04690"
     CDS             11571..12359
                     /locus_tag="PLW53_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36753.1"
                     /translation="MSALLKSELRKLIYLRANWLLLIWSGLFAALGTVAPILVLNSEN
                     GPGRGVFSGTETPEGINAVFANAAGGYLFALILGILIMTNEYRHGTAVGTFLVTPKRS
                     TVLVAKLIAASLAGLVIQLFSFIFAITSGFIALTFFEPHAPVPAELLFKVFRASAISG
                     LVLGIVGVGLGALVKNQIAAVTGAVIWTLIVEGLVVAFVESIGKYLPAGAITGLVDVD
                     FGENDFNFSIENYLTPGPATLVLLVYAALFSLIAMRTTLRRDLD"
     gene            12369..12812
                     /locus_tag="PLW53_04695"
     CDS             12369..12812
                     /locus_tag="PLW53_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRD36754.1"
                     /translation="MSKEVFATYLDDVARVQEFYGAFASRLDNVPSDGGWSASQCLAH
                     ICDTEISLSLRVRMILTSDNYQFSAWDEDAFAAIKKDRDAKTSVETFAALRRNNLDLL
                     AGLPADKLERLGVKANGESIKLIDYLAYMSKHIKAHLEQAVAASR"
     gene            complement(12809..13735)
                     /locus_tag="PLW53_04700"
     CDS             complement(12809..13735)
                     /locus_tag="PLW53_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD36755.1"
                     /translation="MIPAELSLEAASLDDAKDFAEIMTLADQFFTPGAEIVGPTEAEE
                     ILNGYIDSVQARKIVDRASGKAQAFITVHPDNSRKRIYCDTWQRPGANLEGASLEISL
                     ELARGMSSEFDLWIGTNAKDLGYIQALTKRGFNLLRTYHGLKAEITNHPYPKLEKGLE
                     MRLISEDEKKIWWATHQDSFSKHFGFVPRPFEEWKAMIDKAVGVDLNARWVLYLEGQA
                     VGFIECSDIKKDVGAGFVDGIGVIQSQQGNGYGELMLRWAFAYYASIARQSLELNVDT
                     GNESGALRLYEKLGFNVKSSWQHFENKGWATL"
     gene            13758..14267
                     /locus_tag="PLW53_04705"
     CDS             13758..14267
                     /locus_tag="PLW53_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRD36756.1"
                     /translation="MRKLLLFVLAALLTGCATQESSNSSFSADDQMFAAMMIPHHEQA
                     IQMSELALTNSTDPEIIELANEIKAAQGPEIEQMKNWGSSMMGSHAGHMMDEGMLSDD
                     EMAQLKDAKGAEFDRLFLEGMIKHHQGAIEMADMILNSANLEAATLGKNIVESQSAEI
                     TRMRDLLSR"
     gene            14283..15527
                     /locus_tag="PLW53_04710"
     CDS             14283..15527
                     /locus_tag="PLW53_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36757.1"
                     /translation="MSEKVPLGHDFSRIWSASLITNLVDGVLRLAAPLLAVSLTDDPI
                     LIGAMSALGLLPWLFFAIPIGALVDRVNRGKALVLGNGLRAVIALFIAFAVSQEFINI
                     WLLLLAVFLFGICEVLVDTTSQAVLPQILDKSNYERGNSRLQISEVIVAQFAGAPLSG
                     LLYAVSIALPFYFSTSGFVVAALLILILPFKREINVGKKGDQKEEKLGLKGDIKFALN
                     YLYQDKQIFSIVVITTSLGFFYSLSNSIAPLFILKELNVSPALFGVVLAIQGIGALAG
                     SIAAPMASKLLGRGRALALNVFLASFLVILIGISPNAYFFTAVSVVIGFTISVWNILL
                     MSLYQSLIPPNLYGRIHGARRTIVWGLMPIGAIIGGVIARGGLRLPFLVGGVIATLIA
                     FFSYKHIKRIGDLSAEVTNQNN"
     gene            15541..16191
                     /locus_tag="PLW53_04715"
     CDS             15541..16191
                     /locus_tag="PLW53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRD36758.1"
                     /translation="MSSQSLYHRSLYPIIAAIFCALLLISNIGATKFIDFGFIKTDGG
                     AFLFPLVYILGDILTEVYGFKAARRVIYTGFGLGALAGLTFWLVQIAPAASDWPNQGA
                     FESILGFVPQIVIASITAFLLGQLANSWTLVRIKKITNEKKLWARLIGSTAVGELVDT
                     IVFTMIASLGRLSFDEFLNYLVVGYLYKSLVEVALLPITYPVIAYVKKREAEVSSS"
     gene            complement(16188..17414)
                     /gene="tgt"
                     /locus_tag="PLW53_04720"
     CDS             complement(16188..17414)
                     /gene="tgt"
                     /locus_tag="PLW53_04720"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRD36759.1"
                     /translation="MSFSFEVQKEISGQLGRAGVISTPHGQIATPAFIPVGTKASVKS
                     LAPEALTDLGAQAVLANAYHLYLQPGSETLDQAGGLGKFMNWNKPTFTDSGGFQVMSL
                     GAGFKKVIDMSLGSLDSDEAMAESKERLAHVDDEGVTFKSHLDGSMHRFTPEISMQVQ
                     HQIGADIIFAFDELTTLHNTRKYQEQSLERTRLWAIRCLDEHKRLTDQRSDKPYQALF
                     GVLQGAQYEDLRRKAAKDLSEMNSSGISFDGFGIGGALDKASLGQIVSWMSSELPRDK
                     PRHLLGIGEPADLFAGVENGADTFDCVAPSREARTSAVYSENGRFNVSSATYKKDFSP
                     IDKDCNCYTCNNFSRAYLNHLFRAKEMLASTLATIHNLAFTVRLVDRMRQAILDENFI
                     EFKTEFLAKYQAKALD"
     gene            17471..18793
                     /locus_tag="PLW53_04725"
     CDS             17471..18793
                     /locus_tag="PLW53_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36760.1"
                     /translation="MRFKAKEKVALGKNYWRLFSAHAISNLGDGIAAIAYPWLASAVT
                     RSPFLIALVAVASRLPWLIFTLPAGVITDRFNRGKIIVAMDIARGSLALLVAIVVTLE
                     AGSLPKLNEVASLTDLETNWTLYLVLIITALLFGFAEVLRDNSAQTLLPSVVEKEKLE
                     SANGKLWSVEYVTNSFVGPPLGSFLLGIAIFLPFYIDSATFFISAALIATLIPSLKKV
                     DTEKKVEKINFKSEIKEGFTWLWSHELLRPMALILGALNAIGALTAAVFILFAQEILE
                     TSLFIFAVLGTAGAVGGSLGGILGPKISKKLGSGPSLYLTLLSGPIIALIIGLSSSWH
                     LFYLLTAISTIFAVLWNVITVSLRQSIIPSHLLGRVNSVYRFFAWGSIPIGTLLGGAL
                     VDLLELMGDREFALRSPYFLSALLGLALFIFAAPRLTTAKIEAARNPK"
BASE COUNT         5241 a         4082 c         4142 g         5525 t
ORIGIN      
        1 caagtttcat tacgatgccg tttgtatttg ggctgataag aacctgtaaa gttttcccaa
       61 actttgcata attttgtggg cagttacgtg ggcaatcggc aatattgagc gagaatcgaa
      121 agcctccggc atacagggcg actcttcttt ttcaaatata cttttagagt gcacaagatc
      181 tatgagatgc ctgtagctga tgtttatgtt cattatgtaa ataaggttga gcgtaaagat
      241 cgcacagaag ccgaactcat caaagtaatc acctggctca caggatttga ttcaaagaca
      301 ctgaaaaacc acctcaagaa gcagactaca tttaaggaat tctttaaggc agcaaaaatt
      361 caccccaatg cgaagttaat aacaggttca atctgtggcg taaaaatagc tgagatcgaa
      421 gacccactta tgaagaagat tcgttacatg gataaattag tagacgaatt ggcgaagggt
      481 cgcccaatgg aaaagatact tcgttccggc ttatagggcg actcttctct aaagatttct
      541 cactcatatg cctggttgcg ttctagataa tacttaatag taatttttta gtttactttt
      601 gttttttatg gagagttttt accgaatatg gtattaattc cttggcgcat atcgcaatca
      661 tgattccggc aactgcgctg agtagtagcc caacagagcg atcatctaga agcagagaac
      721 cactcatgta aacagcaaag gctcctagcg cttcagctag ggtggcagca agggttaaaa
      781 gcagagccat cttgcggcta tatccagcaa gcagtgccgt tgcacttatt gcaatacctt
      841 caggaatgtt atgcagcgct aggacaagtg atgtagtaag cccggtctga atatcaacca
      901 acgtagctcc aattggagca gccccttctg gaagattatg aagagatagg gcaatagtta
      961 taagaagcgc agggttttta accttagatg catctttatc aaacttaacg ctagatagga
     1021 gaacaactac ggtaaaacca ataccagttg tgagtagtac ctgcataata tttaagccgc
     1081 tatcaagagc tgatggaatc aactgaagag cgcttacagt cagcatcgct ccagcaacta
     1141 gtagtagcgt gatggcgata aagcgatctg atggattttt tttcaaggca atcaatatcc
     1201 agcccaaaag agttgcaccg gctgatagca gagccagaat tagcccaagt tctagagttg
     1261 aaatatcaca gctccttcac tacttactaa accttccttc tttcaagcct actgcctcgc
     1321 ggatttggct caatgttgcg ataatgccag tatgaaacga gtttcaccgc ccaaggctct
     1381 tcgccgcaag agtagtcgtg aatcccgttt ggttgaagta atcaatgatt tagatcaatt
     1441 cttaagcgcc caacagattc ataaaatctt aaatcagcag ggaagttcaa tcggtcttgc
     1501 cactatctat cgccgtcttg agagattaag tcaggagggc aaagtagatg ccctaattag
     1561 ccccagtggc gagcggctct atagacgatg cggacaagat ggcggccatc atcaccactt
     1621 aatctgccgg gtatgcggag ctactcagga atttgatcta gaagcgatag aagttgttac
     1681 tagcaaaatt gcaaagaagc atggctatca agatgtcagc cataacctgg aagtatttgg
     1741 agtatgcgat aggtgtcaga gctcggagac taaatcaact tcaaaaccaa agctgcgata
     1801 aagctttaat gctttttcat tactttcatc ggtatagacc caagcctcac taaatccatc
     1861 agagataatc tgcttgagag cgcaggtaag gattttcttt cctaaaccct gtccctgcat
     1921 ttgagaaatt atcccgatta catagatttc acatactttt ctgccaatta atgtatgttc
     1981 tttaagccag aaaaatccta aatcttttcc gtcttttttc ataaatatga ggcgatctgg
     2041 acggaaccaa acctctccca ttttggcaag gagttttgat agatcccaat cgctctggtc
     2101 tggatggcca atgaagattt gattgtttag gttcaagtat cttgattcat ctttatttct
     2161 aataaaactt tcaagttcaa actctgaatc caaattatct gctgctaaat ttaaatcaac
     2221 taagttgcat ctcagacgca atattggccg cttattggct aaatcgcccc tagtgagatc
     2281 ttccatgggg ctagattaga taaaaagtga ggctttgaga tactcttagg cctgtcttca
     2341 cgcgccttgc gtagagaaaa tccaaagtca ggctttagag ccggcttata tgtaggtctt
     2401 atcttttcgt aaatacgaca agtcactaga gacatacatg tctagatgat tggtctgtat
     2461 tttttatgtc cgttaaacct gcaacaaaag aaataacctt taagtccctt gggctaaacc
     2521 ctgcactcat taaaactctt gatcagcaag gaataactaa tccttttccg attcaggcag
     2581 caaccattcc tgatgcaatt gctggccatg acatattggg ccgaggccag actggctctg
     2641 gaaagactct tgcctttggt ctagctctac ttcataacat ctatggcatg agagcaaagg
     2701 ctcatcaacc actagcactt gtccttgctc cgactagaga gctagcccag caaatagatg
     2761 aggtccttcg ccctcttgca agatctgttg gccatgaatg tgtagtaatt gccggcggaa
     2821 tgccatatgg caaacaggtc aatgccttaa agaaatccat ctctatcgtt gttgcaactc
     2881 caggtcgcct aatagatctt cttgatagaa aagacattcg ctttgatcac cttgagatca
     2941 ctgttctaga tgaggcagat cagatggctg atatgggctt tcttcctgct gttaaagaga
     3001 ttttgaatca aactaaaaaa ggtggacagc gccttctctt ctctgcaact cttgatcgcg
     3061 gagtagatgg cttagttaag caatatctaa aagagcctag atctcactcc attaaggaaa
     3121 ttgaaggaac caaagtcaag gctgagcatt atgtctttgt ctatcgccag ggcgataaat
     3181 caaatatcat caatcagatt gcagcaagac agggaaaaac tctgctattt gttagaacaa
     3241 agcgcggcgc tgataaattg gcagcaaacc ttgcccatgt tggcgttaaa gtcggaactc
     3301 tccatggcgg aaaatcacag gcagtgagaa ctagaactct tgcaagattt aaagattccg
     3361 agagcgcagt cctaattgca actgatgttg ctgctcgagg aatccatgtt gacggcattg
     3421 acttagtagt ccatgttgat cttcctgcag atcataaaga ttatctacat agatctgggc
     3481 gaactgctag agctggagaa tctggaaaag taatctcact tgccacccat caagagcaga
     3541 gaactatggc tggaatagcc ttaagagctg gagttgaaac ttgggttatg gcagttactc
     3601 caatggatga gaagttggta aagattgccg gggcaagaac tcctagtgga attccaactc
     3661 cagaggctaa agaaaaagca gtaacaaaag cgccagagcg cggctcaggg ccaagaccta
     3721 agggcggtcg aaagcgcaag agagttggaa atgatcagac tcgaaacaag agaagcgccc
     3781 cacgggcaaa gcgagctaga taagcctttc tgctagttag actttagtca tgaagggcaa
     3841 gttagttgta acgcttcaat tcattctgct ctttgctttg gctctattgc cgggatctaa
     3901 agatagttcg caacttagat ctgctttggc agctctacta gcacttcttg cagtactcat
     3961 tctgtttcgc gcctttagag atttaggaag cgcgctaaca cctcttcctg agagtaaaga
     4021 gggggcagat ctagtaacaa gtggtatcta ctcaaagata agacatccaa tttattctgc
     4081 tctcttcatt cttgctctag ctgcttatct ctggaaacaa agtggcagtg ttttaattgc
     4141 agccctgcta ttaacttccc tgttgctata taaggcaagg tatgaagatc agattttgag
     4201 agataaattt ccagaaagta ttgagtttca aaaatcaatt ccaccattct ttccaagatt
     4261 tagaaaagga aaaaaaccat gactattggt ttagagcgct tattaaggca tatggcttgg
     4321 gctaatcaat taacgattga gcatctagca acccttcctg aagctgcgtt aaagtctttt
     4381 gccactaatc cagagtggtt tgtcgctgag attactcacc acatagtaga tgcagctgat
     4441 gcttatgcat ttcgcataac tggtaaacat cctgaggtct ctggcgagtc aatccaagag
     4501 ataggcaaaa ttgctgattt ggaagtactt gctaagcagg cagcagctat cgatgctgca
     4561 ctattggaat ctgcaaagct agatgatgag ctacttgaat ttaaaaacta ctcaggaaac
     4621 cttgtaaaaa gatggcgctc cacaattctc tcccaatcaa tccaccatgc aacagagcat
     4681 cgagcacaaa ttgctagcgc ccttgaggcc aagggattta agccggtaaa tcttgatgat
     4741 ttagatctat ggagctatga gattagtaat gggtaagtcc tactaaacta ttgccctgca
     4801 tttaaatcat ttctagaaag gattctgaaa tcatgtgtag cgcagttaaa tgtcgctctt
     4861 gtggcaagtg gacatgggaa ggctgcggag aacatattgc tcaggcactt gctgggataa
     4921 aaaaagatca gatctgcagc tgtcctcgtt aaagttttcc ttgatctagt ttcttaagca
     4981 catcctttgc tcttgatcta acttcatcta aatctttaag tcgatcaaga ccaaatagtt
     5041 gttgggcaat ccggtgattt ccttgaaact gctggcgatg tcgatcgaga aagtcccaat
     5101 agagagttgt aaatggacag gcatcatctc cggtgcgctt ctttggatca aagtaacaac
     5161 ctttgcagtg tgtgctcatt cttgaaatat aggcaccccc tgctgcgtaa ggctttgtca
     5221 tcataattcc accatcggca tgtactccca taccaattac atttggaacc atcacccaat
     5281 cgctagcatc aatgaaaacc tctctcatcc attgcagaaa ttgctgggga ttaatcccag
     5341 cgataagtgc aaagttactc aagatcatta agcgcggaat gtgatgaacc caggcgcgat
     5401 tattgatatc ggtaatcgtg tttttcatgc agttcatatt agttttactg ggatcactaa
     5461 ataattgcgg cagctctcgc tttgcctgca aaccattttt ttggcgatac tcacttccta
     5521 gataccaata catgccattg acatattcac gccagccgat gatctgcctg ataaaggcct
     5581 ctgcagaggc aattgggata tctccctttt caaatctctt aagtgctgcc ttaataactt
     5641 cctcggggtg aagtaggccg ttatttaagt aagggctaag cagtgagtgg tgcagcgccc
     5701 aattctcatc ggtgatggca tcttcatagg gaccaaagtt tgcgaagtgg ttttttagaa
     5761 agttatcgag ttgtttaagt gcgccagctc ttgttgtggc ccaattggta gatgggctat
     5821 gtcctagctc tttagcaacc tctagatcta tttcatctgg ggcgtgggat agatatttag
     5881 gccacttgta gttctttggt ggcggcaagc gattatcctt atcaaagttc caggccccgc
     5941 ctagcggctt tgctttatcc atcaagatat ttagacgaac tctctgtttg cgatagaaat
     6001 tctccattaa atagctcttc tgttcatctg cccatttagc aaagagtgaa cgagaagtta
     6061 gaaagaagtc gttctcaaca aaacttacgc catactcgct caatgattga aaaaggcgat
     6121 gagaggaagg ctctgctgca aaaatcttta catccttata tttctttgaa agctcatcaa
     6181 ggccggcaat tgtgctgcta gctttaaggt attcgacttt aaaccctttt tctcttagct
     6241 cttgagcaaa atgtttagca cttgaaatta agaaataaag ccgttctgga tgccaatttt
     6301 tgccagtagt cattggctca ctttgaatca gcgcaattac atcctttttg gcatcagcgc
     6361 tctttaagac tccgtatttg gcgtttaatt gatcaaagcc aacgtagagg atgcgctcta
     6421 tcatgcgcgc aattgtattg accctgatag attttgctcc atggatttag ataatttgaa
     6481 atttggcctc gataccttcg gtgatactca aaacgatgat tctggagcgc ctatctctgc
     6541 agcagccacg attagaaatg tggttgagca ggccgtgttg gcagatcaat taggcctaga
     6601 tcacttcagc attggcgagc atcacaggga tgactttgca atatcttcgc cagatactgt
     6661 tctggccgga attgcaacta gaactaagca gatcacactc ggcaccggcg ttactgtttt
     6721 atcaagtgat gatccggttc gggtctatca aagatttgcc acaattgatg cgctctcaaa
     6781 tggaagagca gaaattactg cagggcgagg atcatttatc gaatcatttc cactttttgg
     6841 ctatgagcta tctgattatg gcgcgctatt tgaggaaaaa cttgagctat tagttgagat
     6901 attaaaagag aaggcagtga cttggtcagg aactatgcga gcaccgctag ttaatcagga
     6961 gatttatccc aagactgagc gcggcagcat tccacttcga gttggagttg gtggaactcc
     7021 tgagtcagtt gtaagagctg caaggcttaa tgccaaatta gcaattgcca tcattggcgg
     7081 tgaccctgcc cgctttgcgc catttgcaga tctttatcga agttcaatga atgagtttgg
     7141 acatccaatt cgcccaattt ctattcactc acctggtcat atcgctgcct cccaagaaat
     7201 cgcaatcgag gaactatggc cacattatca agtgatgttt ggacgtattg gtagagaacg
     7261 cggctggggg caggtcacaa aagaacattt tattaacgaa gttagatatg gctccttcta
     7321 tgttggaact ccagagcagg tggcaagaaa gattgcctat gcaatgaaat ctattggcgc
     7381 agagcgattt gattttaagt attcaaatgg gcctatggcg cactctaaat tgatgaattc
     7441 aattgagctt tatgccaccc aggtcgttcc tatggtcaag gagatattaa gtgctgatcg
     7501 agcagcaagt agcgctagct caaggtgata ctttttagat tatgaagcgc gtcattgccc
     7561 ctttgaaaga ctttcttcat agagagtcat ctagcggtac tttaattctt atcgcttcag
     7621 cccttggctt acttgcggca aactctggcc ttgcagataa ctattttgct tttctagatc
     7681 ttaaatttca aataggacca tcgagtctgg aattagattt cacaatatta aagagcatca
     7741 actatttact aatgagcctc tttttctttg tcgttggcct tgagattaag cgagagctaa
     7801 cttcaggcca tctagcaact tttaaagcag cggcagctcc actatttgca gccattggcg
     7861 gaatgagcct tccagccatt atttatctag caattgccgg gggagtggaa ccaaatggtt
     7921 gggcagttcc ggtggcaacc gatattgcac ttgccgttgg acttcttgcc ttggtcggaa
     7981 gtagcgcaat tgcctcgctt cgatcatttc tactagcgct agctgtaatt gatgatattg
     8041 gcgcgatatt aattatcgcc ttggtttact caactggagt taacctctcc tggggccttg
     8101 cagggctact aacaatcttt gccatcttgg gatttagaaa gcttggaagt caatctgcat
     8161 tggtctatct gccgctagcc ttatcactct ggtattgcgc atatagattt gggatacatc
     8221 caacaattgc tggagttatt ttgggtctgc tcaccccgaa tattgcacgg accaaatcaa
     8281 atcttaccga tagcgaagat ggatctgttt cggttattga atacttagag cataaatttc
     8341 atccgattag tagctatgta atcgtgccaa tttttgcttt tgcaaatact ggagttgaga
     8401 tttcaagcca gtcaattaac agcgcactta actcagttat tgcatgggga atcttccttg
     8461 gtctagtaat tggaaaacct ctgggagtcc tgcttgcaac catggcggca agaaaaataa
     8521 agattgccga atacccacaa ggtgctagga atctagatct aattgccaca ggaagcgctg
     8581 caggaattgg atttaccgtt gcaatattta ttgctcgctt ggcttttgat agcccagaga
     8641 ttgtgaatct agcaatcttt tcagtgatta tgggatctgt ggtcagtgct ctgattagct
     8701 tgctgatctt taaaactcta aagcgctcaa agatttaatt tttagctagc cagccccaga
     8761 ccgattctgc tcctgagtgg ccggttaaaa agatatagac caaggccaag attgcagcaa
     8821 gggctagaag aattgaaacc agccttctta cgacaggcga gccaatacca gcgcctgttt
     8881 tatccatccg atatcgaatg aatagcagaa ttaaaagtgg aatgaaggta aatggcgcaa
     8941 tctcgccata gctctcatga cgttcaactt ccttgttgag tctttcttca aaagcttctc
     9001 cacttgcttt ggcaagaaac aaagatagtt gtgaaattac tgcaagaatt agaactggaa
     9061 ggccatatct tcttgaaaaa cttggccaat aagacatagc aagagctgca aaggccacta
     9121 gtggcactaa aacaactggg gcatgtgagg ctaaaggatg aagtggaagt ccagcaataa
     9181 gatcaaatgg tccacctaga tcatcgccat caaataattt catagagata agtatagaga
     9241 aagcctagat tcctgctgcg tgaccttcac atcttggatc agaacctgcg atataggttc
     9301 catttggcaa gacttcaagc aattgaactg cagagccatg gccataggcc gaaagctctt
     9361 caacgctatg ccccttttct ttaagggatt gaagaatctc tttctccact ctattttcaa
     9421 cctgcaatac cccttcatac ttctcatcaa gtgatgaacc acctgccttg tttaagtgtt
     9481 gccagcgagg agcttcagca ttttcttgcg gattcatctt caaatcaatg gcattaacaa
     9541 ttagctggaa attgctctgc acttgaatat ttgccccagg agttcctccc atgtaagcaa
     9601 gagatccatc atctcttgtt acagaccagg catttagtgt gtgagcggga cgctttcctg
     9661 gagcaataaa atttggtgac tttggatcaa gtgagaatcc ggttaagcga ttgttaagca
     9721 ggatgccagt tccaggaata acccatgagg ctccgaaacc atgaaagaca ctttgaatcc
     9781 aggagagggc attgccatct cgatctgcaa ctagaaaata ggtggtatca cttccttcac
     9841 tagatactga aggctctttc aaatttgctc tttccatatc aatctggctg cgccttgcag
     9901 taatactgga tgctgcaaag agttcttcca aattaatatc aatatgctct ggatctccaa
     9961 gaagtaagta gcgatctgca aaggcaatct tttttgcctc caccattaag tgaattcgat
    10021 ctgcctcttg catctttgcc aaatcgaaac cctctgcaat taatagctcc tcaagcaaaa
    10081 tcattccttg acttggtggg ggctggccat gaattaaata gtcgcgatat ttaacgctta
    10141 atggcggtag aactcgagtt ctatgttctg ccaaatctct aaggctaaac cagccatcgc
    10201 tagcttcaac tagttttcta gcgatctctc ccaggtagaa ggcatctcgc ccatcttgag
    10261 ctatcgcctc tagggaatct gctaaatcac tttgaatgac taaatcaccg attttcaggt
    10321 tggtatcaaa accaaggctc ttaaaaactt gcgtatctgg aaacatttta agatgacctg
    10381 aaattctttt aacaaatcca gagttagcag caaagccatt tcttgcataa tcaatagcag
    10441 gtgccaaaat ctgatccatc gataaaaggc caaattcttt atgtatctca aaccaagttg
    10501 agacaagtcc tgggacggtt gcgcttttaa atccatgatg atctatttcg tttttgaatt
    10561 gatcagcgct agcgctatgg gcagcctcgc cacttccatt aaatgctaaa ttcttctttc
    10621 ttgctgcgtt atgggatata acaaatgcat caccgccaag atgagatgcg ccaggttcaa
    10681 caactgccaa taccgcactc gttgcaatcg cagcatcacc tgcagatcca cctttagtaa
    10741 gaatttcaat accggcagag actgcaagtg gctgacttgc tgcaacgatg ccatttctgg
    10801 catacacagg ggagcgatgt gtgctctggc tcataaagtg aagggtatag gctctgcgat
    10861 atgtctggtg tgaaaatcga atttaaaaat ttaagtaagc gctacaaaga cgtattggct
    10921 gtctcaaact tctcagccca agttcaatca ggtcgggtta ctggattcct aggcccaaat
    10981 ggagctggca agagcaccgc tctgcgctgc cttttaggtt tagtaaaccc aactggggga
    11041 agcgcggcga tatatggcgg ctcatatagc cagctacaag atccaattca taaagtggga
    11101 gcagtattag atagcagagg ctttcatcca ggtcttactg caaagcagaa cttgaaaatt
    11161 atggcaagtg cctcagatat caacttctct cgcattgatg aagttttagc tcttgttgat
    11221 ctagcaagtg ctgcaaataa aagaacaaag ggattttccc ttggaatgaa gcagcgcctt
    11281 tcactagcaa tagcaatgct tggcgatcct gaggttctca ttcttgatga accagcaaat
    11341 ggccttgatc cacttggaat tgtttggctc cgtgaatttc tccagaaatt agcaggcgaa
    11401 ggaagaacaa tcctggtttc atcacatcaa ctagccgaga tgcaaaatac ggtaagcgat
    11461 gtcctgatca ttaataaggg agtactggta gcgcagggaa ctataaaaga gatctgtaaa
    11521 ggtaaatctc tagaggaagc cttcctagag ttaacgttaa ggaagagcgc atgagcgccc
    11581 tactaaagtc agagcttcgt aagctaattt acctaagagc taattggctc ctacttattt
    11641 ggagcgggtt atttgcagca ctaggaacgg ttgcccctat cttggttctc aattcagaga
    11701 atgggccagg acgaggtgtt ttctctggaa ctgagacgcc agaaggaatc aacgctgttt
    11761 ttgccaatgc tgctggtgga tatctatttg cattaatcct tggaatattg attatgacca
    11821 atgaatatcg ccatggcaca gcggttggaa catttctagt aactccaaag cgaagtaccg
    11881 tattggttgc aaagttgatt gcagcaagtc ttgctggtct ggtgattcag ctattctcat
    11941 ttatctttgc aataacttca ggttttattg cgctgacctt ctttgaacct cacgctccag
    12001 tgccagcaga gctgcttttt aaagttttta gagcatctgc tatctcaggg ttagtcctgg
    12061 gaattgttgg ggtgggactt ggcgctctag taaagaatca gatcgcagct gtaacaggag
    12121 cagttatctg gactttaatt gtcgaaggtt tggttgtggc atttgtggaa tccattggaa
    12181 aatatcttcc agctggggcc ataacaggtc tagttgatgt tgattttggc gaaaatgact
    12241 ttaatttcag cattgaaaat tacttaactc cagggccagc aacccttgta ctcttggtct
    12301 atgccgcgct attttcactc atcgcgatga gaacaacact gcgtcgcgat ctagattagg
    12361 agtaaaaaat gtctaaagag gtcttcgcta cttatctaga tgatgttgca agagttcaag
    12421 aattctatgg cgccttcgca tcaagacttg ataatgtgcc aagtgatggt ggctggagcg
    12481 catcgcagtg cctagctcac atctgtgata ctgagatttc actctcgctt cgagtgcgaa
    12541 tgatcctaac tagcgataac taccagttct cagcttggga tgaagacgcc tttgcagcta
    12601 tcaagaaaga tcgagatgca aaaacaagcg ttgagacttt tgcagccctt cgtcgaaata
    12661 atcttgacct tcttgctggc cttcccgccg ataaattaga gcgcctagga gtaaaggcaa
    12721 atggtgagtc aattaaatta attgattacc ttgcttatat gtccaaacac ataaaggctc
    12781 atctggagca agcagtagca gcaagtcgct agagcgtcgc ccagcctttg ttctcaaagt
    12841 gttgccaaga acttttgaca ttaaagccaa gtttttcata gagcctaagc gcccctgatt
    12901 cattaccagt atcaacgttt agctccagtg attggcgagc aatggatgca tagtaggcaa
    12961 aggcccagcg cagcattaac tctccataac catttccttg ttggctttga ataacaccaa
    13021 taccatcaac aaaacctgct ccaacatctt tcttaatatc actgcactct ataaagccaa
    13081 cggcttgccc ctcgagatag aggacccagc gagcatttag atcaactcca actgccttat
    13141 caatcatcgc cttccactcc tcaaaggggc gtggcacaaa accaaagtgt ttactaaaag
    13201 aatcttgatg agtagcccac caaatcttct tttcatcttc acttatcaat cgcatctcta
    13261 agcctttttc aagtttggga tagggatgat tagttatctc agcttttagg ccatggtagg
    13321 ttcgaagtaa attaaaacct cgtttcgtta gagcttggat gtaacccaag tctttggcat
    13381 tggttccaat ccagagatca aattcactac tcatacctct ggcaagctct agcgagatct
    13441 caaggctcgc accttcgaga tttgcgccag gcctttgcca ggtgtcgcag taaattctct
    13501 ttcgagagtt atctggatgc acggtaataa aggcctgagc tttgccactg gcccgatcta
    13561 ctattttgcg agcctgcact gaatcgatat agccattgag aatttcctca gcctcagttg
    13621 ggcccacaat ttcggcacct ggggtaaaga attgatcggc cagcgtcatt atttcggcga
    13681 agtctttcgc atcatcaagg gatgctgcct caaggcttaa ctctgcagga atcacagttt
    13741 tatcttacta tcgcgctatg agaaaacttt tactctttgt tcttgccgcc ctattaactg
    13801 gctgtgctac ccaagagagt tctaactctt catttagcgc agatgaccaa atgtttgcgg
    13861 ccatgatgat ccctcatcac gagcaagcaa tccagatgtc agagttagca ttgactaatt
    13921 caacagatcc agaaattatc gaattagcaa atgagataaa agctgctcaa ggtcctgaga
    13981 ttgaacagat gaagaattgg ggttcatcca tgatgggatc acatgctgga catatgatgg
    14041 atgagggaat gctgagcgat gatgagatgg cgcaattaaa agatgctaaa ggggccgaat
    14101 ttgatcgttt gtttcttgaa ggaatgatta agcatcatca aggggcaatt gaaatggccg
    14161 atatgattct aaattctgcg aatcttgagg cagctacgct aggcaaaaat attgttgaat
    14221 cacagagcgc agaaatcaca agaatgaggg atttactaag ccgttagttt agactcaagg
    14281 ctgtgagtga aaaagttcct cttggccatg atttttctcg tatctggtct gccagcttaa
    14341 ttactaactt agtcgatggc gtcctaagac ttgctgctcc gctccttgct gtttcactta
    14401 ctgacgatcc aattctgatt ggagcaatga gcgcccttgg attactgcct tggctctttt
    14461 ttgctattcc gattggggca ttagttgacc gtgtaaatcg gggcaaagcg ctggtgctag
    14521 gaaatggcct aagggcggta attgcgctct ttattgcatt tgctgtttcg caagaattta
    14581 tcaatatctg gctcttgctc cttgcagtct ttctctttgg catctgtgaa gttctagttg
    14641 ataccacctc gcaggccgtg ttgcctcaaa ttcttgataa atctaattat gaaaggggaa
    14701 actctagact tcaaatatca gaggtaattg tggcccaatt tgctggggcg ccacttagtg
    14761 gcttgcttta cgccgtatca attgccttgc ctttttactt ctctacttca ggttttgttg
    14821 ttgcagcact tttaatcctt attctcccat ttaaacgcga aattaatgtg ggaaagaaag
    14881 gtgatcaaaa ggaggaaaaa cttggattaa agggagatat aaagtttgca cttaattatc
    14941 tctatcaaga taagcagata tttagtatcg tcgttattac tacctcactc ggcttttttt
    15001 attccctatc aaattcaatt gcgccacttt ttattctaaa agaacttaat gtctccccag
    15061 ctctgtttgg agtggtctta gcgattcaag gaattggtgc acttgctggt tcaattgcgg
    15121 cgccgatggc ctccaaactt cttggtcggg gccgagcact tgctcttaat gtctttttag
    15181 cctcttttct ggtaatcctc atcggaatat cgccaaatgc ctatttcttt actgcagtta
    15241 gcgtagttat tggatttacc atttcagtct ggaacatttt gttaatgtct ctatatcaat
    15301 cgctgatccc accaaatcta tatggacgaa ttcatggcgc caggcgaaca attgtctggg
    15361 gattaatgcc aattggggcg ataatcggag gggttattgc tcgtggcgga cttcgccttc
    15421 catttctagt cggaggcgta attgcaaccc tgattgcctt tttcagttat aaacacataa
    15481 agcgaattgg tgatctctca gcagaagtca caaatcaaaa taattagata gcattaatag
    15541 atgagtagtc aaagtcttta tcaccgcagc ctctatccaa taattgccgc tattttctgt
    15601 gcgcttttat taatctctaa tatcggggca accaaattca ttgattttgg ctttataaag
    15661 accgatggcg gagctttctt atttccgctg gtctatatct tgggcgatat tctcaccgag
    15721 gtttacggct ttaaggcagc acggcgggtg atctataccg gctttggtct gggcgctcta
    15781 gctgggctta ccttctggtt agtacagatt gctcctgccg catctgattg gccaaatcaa
    15841 ggagcctttg aatcaattct tggttttgtc cctcagattg tgatcgcaag tattactgcc
    15901 ttccttcttg gccaattagc aaactcatgg actttagtaa gaataaagaa gattactaat
    15961 gagaagaaat tatgggccag attaattggg tccactgcag ttggtgagtt agtcgacacc
    16021 atcgtattta cgatgattgc atctcttggt cgattgagtt ttgatgagtt cctgaattac
    16081 ttagtggttg ggtatctcta taagagctta gttgaggtag cgctcctgcc aattacctat
    16141 ccagtaattg cctatgtgaa aaagcgtgag gctgaagtaa gttctagtta atccaaggct
    16201 tttgcttgat acttagccag aaactctgtt ttaaactcta tgaaattttc atcaaggatt
    16261 gcctgacgca ttcgatcaac tagtcttacc gtaaatgcta agttatgaat ggttgcaaga
    16321 gttgaagcca acatctcttt tgctctaaag agatgattta aataggccct agagaagttg
    16381 ttgcaggtat agcagttgca atctttatca atgggggaga agtctttttt ataggtagca
    16441 gaggaaacat tgaagcgacc attttcgcta taaaccgcgc tagttcttgc ctcccttgaa
    16501 ggagctacgc aatcaaatgt gtctgcgcca ttttctactc cagcaaagag atctgctggc
    16561 tcgccaattc ctagtaaatg gcggggttta tctctaggta gttcagagct catccaagag
    16621 acaatttggc ctagtgatgc tttatctagc gcgccgccga ttccaaagcc atcaaatgaa
    16681 ataccagatg aattcatctc tgataagtct ttagcggcct ttcttctcaa atcttcatac
    16741 tgcgcgcctt gcagcacccc aaatagggct tgatagggct tatcacttcg ctgatcagtt
    16801 aatcgcttat gttcatcaag acagcgaatc gcccagagcc tggtgcgctc aagtgattgc
    16861 tcttgatatt ttctagtgtt atgaagagta gttagctcat caaaggcaaa aataatatct
    16921 gcgcctattt gatgctgaac ttgcattgag atctcaggtg tgaagcgatg catagagcca
    16981 tctaggtgag atttaaaagt tacaccttca tcatcaacat gagccaggcg ctctttactc
    17041 tctgccatag cttcatctga atcaagtgaa ccaaggctca tatcaataac ttttttaaat
    17101 cctgctccta gcgacataac ttgaaatccg ccgctatctg taaaggttgg cttattccaa
    17161 ttcatgaatt ttccaagacc gccggcttga tctagcgtct cagatccagg ctgaagatat
    17221 agatggtatg catttgcaag gacggcttgt gcgccaagat ctgttagtgc ttctggtgca
    17281 agagatttaa cacttgcttt tgttcccact ggaataaagg caggggttgc aatctggcca
    17341 tgaggggttg aaatcactcc agctctacca agctgacccg atatttcttt ttgcacctca
    17401 aatgagaaag acatagcgct caattcaacc acttattagc tattgcaatt agctggggta
    17461 gggttttcaa gtgagattta aagcaaaaga gaaggtcgcg ttaggcaaga attactggcg
    17521 cctttttagc gcacatgcca ttagcaacct tggagatgga atagcggcta ttgcctatcc
    17581 ctggcttgca tcagcagtta ctcgctcgcc ctttcttatt gcattggtgg cagtggcttc
    17641 aaggcttcct tggttgatct tcacccttcc agcaggggta attaccgatc gattcaatcg
    17701 cggcaaaatc attgttgcca tggatattgc gcggggaagc ttggcacttc tggtcgcaat
    17761 tgtggtcaca ttggaggcag gatcccttcc taagttaaat gaggttgcat ctcttactga
    17821 tcttgaaact aattggactc tttacttggt cctaattatc actgcccttt tgtttggctt
    17881 tgccgaagtg ctccgagata acagcgctca aactcttctg ccttctgtcg ttgaaaaaga
    17941 aaagcttgag agcgctaatg gaaaactctg gtcagttgaa tatgtaacta atagttttgt
    18001 tgggcctcca ctgggaagct tcctacttgg cattgctatt tttcttccct tctatattga
    18061 ctctgctact ttctttatct cagctgctct aattgcaacg ctaattccct cactaaaaaa
    18121 agttgatact gagaaaaaag ttgagaagat aaattttaaa tcagaaatta aagagggctt
    18181 cacttggctt tggagccatg aactacttag acccatggcg ctgatacttg gtgcgctaaa
    18241 tgcaatcgga gctttgacag ctgccgtatt tattttattt gctcaagaaa tcctagagac
    18301 atcactattt atatttgcag tccttggaac agcaggagcg gttggaggaa gccttggcgg
    18361 aatacttgga ccaaagatct caaagaaact aggaagtggt ccttctcttt atctaactct
    18421 tctaagtggc ccaataattg ccttaataat tggactatct tcttcttggc atcttttcta
    18481 tttactaacc gccatttcaa caatctttgc agtgctttgg aatgtcataa ctgtttcact
    18541 tcgtcagagc atcatcccta gccatcttct gggaagagtt aatagcgtct atcgcttctt
    18601 tgcttggggc agcattccaa tcggaaccct gcttggggga gccttagtag atcttttgga
    18661 gctcatgggg gatagagaat tcgcccttag aagcccttat tttctctcag ctctcttggg
    18721 tctggcctta ttcatttttg ctgctccaag gcttaccact gcaaagatcg aggcggctag
    18781 aaacccaaag taaggctcaa tcacgcttga ttgaggcaat ttggtaacga aatagatacc
    18841 tgtgcgacat tgattaaaaa gggtcaaata gggcaggcta accccaccct aaggatgcgg
    18901 gtgtggggga agtcgacttt ccatacctag tccttaattt ctatcactag gagaagaaat
    18961 gaaattaaat cgcattgcca aggtagcgat
//
LOCUS       DAOROD010000020        18850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_103_length_18850_cov_39.392072, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000020 DAOROD010000000
VERSION     DAOROD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_18850_cov_39.392072"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..588
                     /locus_tag="PLW53_04730"
     CDS             55..588
                     /locus_tag="PLW53_04730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD36761.1"
                     /translation="MVNDSMPPITQATLHTSMGDIVIELFPNHAPKTVANFVELAAGM
                     REWVNPNTGEKTNQPLYDGTVFHRVIKGFMIQGGDPLGTGTGGPGYRFADEFHPELSF
                     NKPYLLAMANAGPGTNGSQFFITVAPTTWLDRKHSIFGEVKNEASQKIVQAISEVKVG
                     GADRPVQAVKITSVSLA"
     gene            594..1118
                     /locus_tag="PLW53_04735"
     CDS             594..1118
                     /locus_tag="PLW53_04735"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRD36762.1"
                     /translation="MRRTITGSLLLIIAVSYLLQITTVGYEDRFLLNRFYVENGEYYR
                     LFTVALLHGGLWHLAFNLLALYALGTPLENYFGKGRYLLILFISLICGSTLSVLLNPA
                     YVYSIGASGMIFGLLGALALVGEKAGVEWRSILIIVGLNFAIGFVLGGVDWRGHLGGL
                     IGGALVTLTLNRSK"
     gene            complement(1071..3137)
                     /locus_tag="PLW53_04740"
     CDS             complement(1071..3137)
                     /locus_tag="PLW53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRD36763.1"
                     /translation="MMRSVFGLSAYLITVIAIVAITIRSLELYRRIKAGQVDPTRSNQ
                     KAKRFVLMTKEVLTHAKMLKFTGSGIAHWFVMLGFFALSGTLLTAYGQVINPEFALPL
                     IGHWVVYNLFTEGFAWLTGISIVTLIGIRQVTRFTNKGRSSRFYGSGSWKAYYVEGTI
                     LAIVFCVITLRGLEGALIGYSGNPWDFALSKPAVNAFADLDKESIENWIVVVASLKII
                     VSMLWFIVIAANLTMGVAWHRFLAPFNIFFKRELSEKPTLGALPEMISNGKVIDFENP
                     KDDDLFGISDAASISWKGLLDMASCTECGRCQSQCPAWHTDKPLSPKLLIMALRDHAL
                     ANIPKEGNVVGQTISPDVLWSCTTCGACVNECPVDIEHIDHIVNMRRFQVLVESEFPT
                     ELGGTFRNLEKAGNPWGANRADRNAWIAECDFPVEVITGALPDEVEYLFWVGCAGAYE
                     ERAKKTTKAVAELLYISGVKFAVLGARETCTGDPARRAGNEFLYQILARENIDTFNEV
                     YANYKGKKKVVVTCPHCFTTIGRDYKQQGFELEMLHHTQLLNTLVKTGRLKPVTQSSN
                     SLTYHDPCYLGRHNQIYEPPRELLESTGVELIEMPRNQERSFCCGAGGGRMWMEEKLG
                     TRINMNRVEEAINTGAKEVAVACPFCRVMVSDGVTAKGSDTNVIDVAQVLLRSVKSES
                     NQSPTN"
     gene            complement(3242..3502)
                     /locus_tag="PLW53_04745"
     CDS             complement(3242..3502)
                     /locus_tag="PLW53_04745"
                     /inference="COORDINATES: protein motif:HMM:NF018487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HRD36764.1"
                     /translation="MPKSRLRKKKKDNRPEILLAERDPVEMESPAWLAPTMIGAFLIG
                     LFWIVIFYVSQTQYPIPNIGAWNMVVGFAFIGVGFSLATRWR"
     gene            3533..4159
                     /locus_tag="PLW53_04750"
     CDS             3533..4159
                     /locus_tag="PLW53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF005849.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRD36765.1"
                     /translation="MGTRILVVDNYDSFVFNLVQYLQQLGAECTVVRNDEVSASLADQ
                     YDGVLISPGPGTPEEAGISIAMIKYAAEINKPLLGVCLGHQGIGVAYGATVNRAPELL
                     HGKTSVITHNNSGVLKDIPNNFTATRYHSLAIDEKSLPEEIEITARSESGLVMAIRHK
                     TLPIHGVQFHPESVLTEHGYQMLGNWLVECGDSSARERSEGLSPVVAR"
     gene            complement(4156..5721)
                     /locus_tag="PLW53_04755"
     CDS             complement(4156..5721)
                     /locus_tag="PLW53_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRD36766.1"
                     /translation="MKKILGERYELGAMIGTGGMADVYLAQDVRLNRQVAIKILRSDL
                     ARDPSFVTRFNKEALSVAALNHPGIVSVYDSGKEDSPSGAMPYIVMEYVEGKTLREIV
                     NKGERFALNRAVEIAEGILIALQYSHKNGIIHRDIKPGNIMITDNGDVKVMDFGIARA
                     LADTGATMTSTWNIIGTAQYLSPEQATGTQADARSDLYSVGCLLYELLAGRPPFTGDT
                     PVAIAYQHVSAPLVPITEIQESLDPALNAFFSIALAKDANERYQSANAMLKDLKKLIK
                     GEGITTQIPKQLRKEKARSKVAALSIALILLVGGVSYFIFNPNQSAVTRVLPNVVGLT
                     EAQARQELAGLTITIQRAPDPRIPKDRVASQLPLATTRISDGSAVTLTISDGPGDAVV
                     PTGLLGKPLEEVRAALAAAGLRVSRTIAIDSEQEPGIVLKIIPEPGSTVEAGTGVVLE
                     IASGNISVPSLVGLNEIEARTILTQAGFLVKIVEAYDANQVIGSVLAQAPSAGEIKLI
                     GSSVTITINKTQG"
     gene            complement(5718..6497)
                     /locus_tag="PLW53_04760"
     CDS             complement(5718..6497)
                     /locus_tag="PLW53_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRD36767.1"
                     /translation="MAVKLNFSDIAWRSEVGLQRQGNEDSALVSNSLLAVADGMGGYV
                     GGEIASRTVIEKLIQLLPTLENPELDNESREDLLSSSIESMDAAIAEIGAARPELVGM
                     GTTLTSIALFDSNLLLLHLGDSRAYRLRGKKIEQLSHDHTVVQELIDQGRLSPDEIAD
                     HPQRSFLTQALMGKENLSPILLAYPVLKGDRYLLCSDGLTAVLDEAQIAKAMQQDLIS
                     AVNSLIELTYKAGAPDNVTVIVAEIGESKASIKTQGFGAAL"
     gene            6578..7498
                     /gene="pheA"
                     /locus_tag="PLW53_04765"
     CDS             6578..7498
                     /gene="pheA"
                     /locus_tag="PLW53_04765"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRD36768.1"
                     /translation="MRRYAYLGPAGTFTEEALRTIAEPSDELHPYANVTAALNAVRNS
                     QADQALVPIENSVEGVVARTLDELAIGEPLVIYREITLPVSFSLLVAKGKESSQVKKI
                     ATHPHAEAQCRSFIAKNYPDAEIIPTSSTAAAAADLVKSGFDAAIASPAAAKEYGLSA
                     IANNIGDNDGAVTRFVLAGKPGLVPALSGHDRTSLVAFIGTDHAGALLEVLTEFSMRG
                     VNLTFIQSRPTGKELGSYHFIIDVEGHINEERVGDALTGLRRICEDVRYLGSYPRADK
                     IAPTTTTKTADNSFKQADAWLSAVRAGEKI"
     gene            7499..8764
                     /gene="serS"
                     /locus_tag="PLW53_04770"
     CDS             7499..8764
                     /gene="serS"
                     /locus_tag="PLW53_04770"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRD36769.1"
                     /translation="MIDIKVLRESPDLVRASQSARGEDVSLVDRVLAADEIRRSAIVE
                     FEALKAEQNTLSKSVGSAKGEEKAALLEKAKALSDAVKAAESKKNATEAEYKSIAMSL
                     SNIVDKAAPVGGEADFKVLEEVGRPREFNFTPKDHVELGKILGAIDVERAAKVSGARF
                     YYLTGVGALLELALVNYAITSASKAGFIPVIPPVLVKPAAMEGTGFLGQAAENVFHLK
                     DEDFYLVGTSEVALAALHMDEILDAKSLPIRYAGYSPCFRKEAGSYGKDTRGIIRVHQ
                     FDKVEMFSYCALQEAEAEHQKLLNWEKDFLNAMEIPYRVIDVATGDLGSSANRKFDCE
                     AWIPTQNAYREVTSTSNCSDFQARRLNIRIKTESATTPVATLNGTLVAIPRMIVAILE
                     NHQQSDGSVLLPKALQGLLGMERFTPVAN"
     gene            8761..9582
                     /locus_tag="PLW53_04775"
     CDS             8761..9582
                     /locus_tag="PLW53_04775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD36770.1"
                     /translation="MSAVTRRPKLIATDLDGTIVAHYGKISERTKAAFNAAHNLGVEI
                     FYVTGRPPRWMPEIAKVFPFGHGICANGALLYDIHNERVLEEWAMSVDSQIETVNRLR
                     RSIPEISFAVEWHNYFLREKKYVPRWDVGQDNVGVDDITEIIDSPAIKMMARCSNQEL
                     SSDQMLRIAQLELEGVATVTHSNPHDSLLEINAHGISKGETLSKIAARYGISADETVA
                     FGDNPNDFSMLAWASRSWAMADGHPDAVKYAKFVAEPHEEDGVAKEIEKLLELPE"
     gene            9579..10226
                     /locus_tag="PLW53_04780"
     CDS             9579..10226
                     /locus_tag="PLW53_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HRD36771.1"
                     /translation="MNTALAVPLWLDLLTVGIGALQGALFAIFYKRFDLVGVISVAIL
                     TGLGGGILRDLLLAVGRPAAMQDKYILTAIASCLVAILIGRWYKRASSTVVFLDSVAM
                     SLFAVAGTYKAIVNGTSELVAILLGVLTAVGGGVLRDVVCRITPQIFTGGPLYATASA
                     VGATLFVLLEKSAIDLNVALAVSAITIVAIQMASLRFRIHLKPVLDSLDFEDPSK"
     gene            10243..10328
                     /locus_tag="PLW53_04785"
     tRNA            10243..10328
                     /locus_tag="PLW53_04785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10277..10279,aa:Ser,seq:tga)
     gene            10449..11252
                     /locus_tag="PLW53_04790"
     CDS             10449..11252
                     /locus_tag="PLW53_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36772.1"
                     /translation="MATGVFSTTRAAIKERTLRTDRWWLQPLIIVAVLISFIIYATFR
                     AFENKYYFAEPLISPFYSPCLSTVCVEGAAHFGTPIGAFTIFGFLISPALFILIFPLG
                     FRLTCYYYRKSYYRSFWMSPPACAVAEPHSKYTGETRAPLILQNGHRWFFYAGLVLNV
                     ILTYDAIIAFKNPEGEWGHMSVGTLVLLLNATMLWLYSASCHTCRHTIGGRLRNFSKH
                     PIRYKLWSWVSVLNHSHPTYAWISLFVVAFTDFYIRLVATGTITNYYFF"
     gene            11260..13155
                     /locus_tag="PLW53_04795"
     CDS             11260..13155
                     /locus_tag="PLW53_04795"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRD36773.1"
                     /translation="MNNDIKFSRYNFDVVVIGAGGAGLRAAVEARQAGLRVAIICKSL
                     FGKAHTVMAEGGAAAAMGNVNDNDGWKVHFRDTMRGGKFLNNWRMAELHAKESPDRIW
                     ELETWGALFDRTKDGRISQRNFGGHEYPRLAHVGDRTGLELIRTMQQKIVSMQQADAK
                     EHGDAEKYLKVFAELTITEILKDGEKVSGCYGYWRETGDEVLFEAPAVVLATGGIGKS
                     FKITSNSWEGTGDGHALALKAGSALVDMEFVQFHPTGMVWPPSVRGILVTESVRGDGG
                     VLTNSEGKRFMFNYIPEVFKDKYADNEAEADRWYKDQENNRRPPELLPRDEVARAINS
                     EVKAGRGSPRGGVYLDVSKRLPADEIKRRLPSMWHQFKELADVDITEQPMEVGPTCHY
                     VMGGVKVDPDTAAAYQVPGLFAAGEVAGGMHGSNRLGGNSLSDLLVFGRRAGAGAAEY
                     VKKLASNRPAVSDKEIARAHSHLNEPFTRDGNENPYALHDELQNVTQDLVGIIRNEKE
                     LSDALVKLESIRKRAAQVKASGGRAFNPGFHLALDLENMLLVSESIARSALARQESRG
                     GHTRDDYPKMDANWRTINHLSKWNGKQVEIDPAKSQMLPAELIEVFEMDELKKYYTEA
                     ELAKGGN"
     gene            13155..13916
                     /locus_tag="PLW53_04800"
     CDS             13155..13916
                     /locus_tag="PLW53_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRD36774.1"
                     /translation="MSRKVKLRIWRGDAKEGKLEDVSVEANEGEVVLDVIHRVQGTQM
                     PDLAVRWNCKAGKCGSCSMEINGKPRLACMTRMNTFAEDETITVTPLRAFPVIRDLVT
                     DVSYNYRKAMEIPSFAAPEDLKPGEYRMAQVDVERSQEFRKCIECFLCQDTCHVIRDH
                     EENKKSFAGPRFFIRIAELDMHPLDRLRNRKQKAQEEHGLGMCNITKCCTEVCPEHIK
                     ITDNAIIPMKERVVDVKYDPIRWLGSKIRKREGIS"
     gene            13916..14260
                     /locus_tag="PLW53_04805"
     CDS             13916..14260
                     /locus_tag="PLW53_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRD36775.1"
                     /translation="MNLVKRWAILALAFWLTTFIVSGIEIQDGAWNYFWVAALFGVVN
                     TLLGGLLKLFTLPAVILTFGLFVFVINAAMLTLVDRWSDVLTIDKFTSALVGALIISL
                     ISGFTNKLINKA"
     gene            14272..14598
                     /locus_tag="PLW53_04810"
     CDS             14272..14598
                     /locus_tag="PLW53_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRD36776.1"
                     /translation="MKRLLAVGLGGALGSLFRYLTVSKGDEIGLFLVNIFGVAVAGFI
                     ALRLSTSELIRLALIPGFAAGLTTFSSVAVIHVEDNSLRSIAYFFGSVLVSLLILFAI
                     KPKVKI"
     gene            14595..14930
                     /locus_tag="PLW53_04815"
     CDS             14595..14930
                     /locus_tag="PLW53_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRD36777.1"
                     /translation="MRVSYFLLGAFVGAPVRFVIVEYLKSYLKFPLGILLVNVIGSFL
                     LGLILESDTNIAFALMGFSGALTTWSAFAMDLFEQAKRREQLLFLLNLFGNYALALLA
                     AGLGIWIAQ"
     gene            14938..15861
                     /locus_tag="PLW53_04820"
     CDS             14938..15861
                     /locus_tag="PLW53_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36778.1"
                     /translation="MKKSALFLTLSLLLASCSSQEEVVAPELPSVPSNCADTKVLESI
                     LPRIADAKYIETEWEPAEGTDLYAAYNAGGIACTYGLQEAEVGATILWAPDNKILFSE
                     LTPNWIGFGQKEIDLPGIEEEAAYYLSEGIEGQGEYHIWSVNLLINGSWIQVGATFFN
                     SLEDAIPVIKAAIDSLRTPKRAEKEKIMGCYLAELPNDLYVFNVHYHDNNTISADFYY
                     KNINGEPSKGLFLGTYTNGIARGFYSLPTSNGTSERELFLKGDKNGFVTATAKLEKVG
                     SIEKYVRPLDIKWSQEIKFIPAQECEGLLRG"
     gene            complement(15847..17304)
                     /locus_tag="PLW53_04825"
     CDS             complement(15847..17304)
                     /locus_tag="PLW53_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRD36779.1"
                     /translation="MSTFDKEVGRKLPSRPHDIPTPEPLMKFMEQGWAPSPLENLQKS
                     NAADFAAKRRELLSKAFPGKRLVIPAGTFKVRSNDSDYRFRPHTAFSWLTGIGGSDAV
                     PDSILILEPSGSSHESLLFIHPRSPRERSDEFYRNPRHGEFWIGRRMTLAESELVYGI
                     TVRHIDSLDDFLTSEKETLIIRGEDNYIDSRVRENSGDEELASWVSEARLVKDEYEIA
                     EMRKAIDATHRGFEDMIKQIPSAIGKKRGERLIEGAFFTRARFEGNDLGYDSIVAAGS
                     HACVLHWIRNDGQLRDGELLLIDAGVEMDSLFTADITRTFPINGRFTPAQRKIYTLVY
                     EAQKAGMKAVKPGAKFRDFHIAASEVLARGLEEMGVLPIPAQESLKPDVGLHRRWTVH
                     GTGHMLGMDVHDCAKARKEAHTEGVLKEGMILTVEPGLYIQPDDTLFPAEYRGIGVRI
                     EDDVLVTKDGCENLSSAMPSHPDEVESWMAALTSK"
     gene            17370..17458
                     /locus_tag="PLW53_04830"
     tRNA            17370..17458
                     /locus_tag="PLW53_04830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17406..17408,aa:Ser,seq:gct)
     gene            17460..18770
                     /locus_tag="PLW53_04835"
     CDS             17460..18770
                     /locus_tag="PLW53_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD36780.1"
                     /translation="MAEFRIAIIGAGPAGYFAAQALQSAEIDGKTFAIDMIEKLPTPW
                     GLVRSGVAPDHPKIKSVSKVFEKIAAQEGFRLIGNVELGRDISLKEIEELYDAVIIAT
                     GSTVGRKLGIPGEELANSISAADFVPWYNAHPEYKNLEVDFSNRVAVVIGAGNVAIDV
                     ARMLALNPKELDPTDTAQYALDKLHNSAIREVHLFGRRGAEHAAFTSPELRELPKLEY
                     TDVFIDENEISAAQQRASSAGEIPKDVKANLEAMAAIATADKKGVDRKLSLHFLATPL
                     EIKGKDRVEEITFSVNEVKDGQVVPTGKTHSVKCGLVISAIGYRCLELPGLVYESGKI
                     KNTDGRIGSSNTYVVGWAKRGPTGVIGTNKSDSSEVIKLLISNLTTPKNSRDFLEILS
                     SRNITYISQKGWEQINNAEISAGEAQGKPRVKITDIQEMLGFAG"
     gene            18773..18845
                     /locus_tag="PLW53_04840"
     tRNA            18773..18845
                     /locus_tag="PLW53_04840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18806..18808,aa:Arg,seq:acg)
BASE COUNT         5334 a         3943 c         4194 g         5379 t
ORIGIN      
        1 agagcacctg ctttgcaagc agggggttag gggttcgatt cccctagtct ccacatggtt
       61 aatgattcaa tgccgccaat tactcaagcg acccttcata cctcaatggg cgacattgtt
      121 attgaactat ttccaaatca tgcaccaaaa actgttgcaa actttgttga gctagccgca
      181 ggaatgcgtg agtgggttaa tccaaatact ggcgagaaaa ccaatcagcc actttatgac
      241 ggcacggtat ttcatcgagt aattaaaggt ttcatgattc aaggtggaga tccactcgga
      301 actggaacag gtggaccagg ttatagattt gcagacgaat ttcatcctga gttatctttt
      361 aacaaaccat atctattagc gatggcaaat gctggtcctg gaacaaatgg ttcacaattt
      421 tttataactg ttgcaccaac tacttggtta gatcgcaaac acagtatttt tggtgaagtt
      481 aagaatgaag caagtcaaaa gattgttcaa gcaatatcag aggttaaagt tggtggagca
      541 gatcgcccag ttcaagcggt gaagataact tcagtatcgc tggcataaat ttaatgcgcc
      601 gcactataac tggctcacta ctattaataa tcgctgtctc atacttatta caaataacca
      661 cagttgggta tgaagatagg ttcttactca atcgtttcta tgtcgaaaat ggtgagtatt
      721 accgcttatt tactgttgct ctacttcacg gtggactatg gcatctagcc tttaatttac
      781 ttgcgcttta cgcgctagga acgccgcttg aaaactactt tggcaagggt agatatctct
      841 taattttatt tatctctcta atttgtggct caactctttc ggttctatta aaccctgctt
      901 atgtttattc aataggggca tcagggatga tttttgggct cttgggcgcc cttgctttag
      961 ttggcgaaaa agctggagtt gaatggagat caattttaat tatcgttggg cttaattttg
     1021 ctatcggttt tgttttaggc ggagtggatt ggcgaggaca cctaggaggt ttaattggtg
     1081 gggctttggt tactctcact cttaacagat ctaagtaata cctgagccac atcaataaca
     1141 tttgtatctg agcctttagc agttactcca tcgctaacca ttactcgaca gaatgggcaa
     1201 gccacagcaa cttccttagc gccagtatta atcgcttcct ccactcggtt catattaatt
     1261 cgagtaccta atttttcttc catccacatt cttccgccgc ctgcgccgca acaaaaagat
     1321 cgctcttggt ttcttggcat ctcaattaat tcaactccag ttgattccaa taactctctt
     1381 ggtggttcat aaatttggtt atgccggcct aggtagcaag gatcgtgata ggtaagagag
     1441 ttactgctct gagttactgg ttttaaacgt cctgttttga ctaaagtatt tagcaattgt
     1501 gtgtgatgca acatctctaa ttcaaagcct tgttgtttat agtcccggcc gattgttgtg
     1561 aagcaatgtg ggcaggtcac cacgaccttc ttttttccct tgtaatttgc ataaacctca
     1621 ttgaaggtat caatattttc tcttgcgaga atttgataga gaaattcatt tccagcccta
     1681 cgcgcaggat ctccagtgca ggtttctctt gctccaagga ctgcaaattt aacccctgag
     1741 atatagagaa gttctgcaac agcctttgtg gttttcttcg ctctctcttc atatgctcca
     1801 gcgcatccaa cccagaagag atactcaact tcatccggaa gagctcctgt aattacctcg
     1861 actggaaaat cacattctgc aatccaagca tttctatcag ccctatttgc tccccaggga
     1921 tttccagctt tttcaagatt cctaaatgtt ccaccaagtt ctgttggaaa ttctgattca
     1981 actaaaactt gaaagcgtct catattgact atgtgatcga tatgttcgat atcaaccggg
     2041 cattcattaa cgcatgcgcc gcaagttgtg caggaccaga gaacatcagg tgagattgtc
     2101 tggccaacaa catttccttc tttaggaata tttgcaaggg cgtgatctct aagggccatg
     2161 attaacaact tgggtgatag cggtttatcg gtatgccaag cagggcattg ggattggcat
     2221 ctgccgcact ctgtgcagct ggccatatcc aatagacctt tccaggatat ggatgcagca
     2281 tcactaatac cgaagaggtc atcatcttta ggattttcaa agtcaattac ttttccattt
     2341 gaaatcatct caggaagagc gcctagcgta ggtttttctg aaagctcgcg cttaaaaaag
     2401 atattaaatg gtgctaggaa tctatgccaa gcaacaccca tagttaaatt ggcagcaata
     2461 acaataaacc agagcataga aacaataatt tttagcgatg caactaccac aatccaattt
     2521 tcaatacttt ctttatcaag atcagcaaag gcattaactg caggttttga cagcgcaaaa
     2581 tcccagggat ttccgctata accaattaac gctccttcaa gtccgcgcag cgttattaca
     2641 cagaaaacaa tggccagaat tgttccttca acataatagg ctttccaaga accagagcca
     2701 taaaaacgag agctacgacc cttattagta aatcttgtga cttgtcgaat tccaattaat
     2761 gtaactattg atattccagt aagccaagca aaaccttcag tgaataggtt atagacaacc
     2821 caatgtccaa ttaaaggcag tgcaaattca ggattaataa cttggccata tgcagtaaga
     2881 agagttcctg agagtgcgaa gaaacctaac attacaaacc aatgcgcaat gccagagcct
     2941 gtgaacttca acatctttgc atgggttaaa acttcttttg tcattagaac aaaacgttta
     3001 gctttttgat ttgatctagt tggatcgact tggcctgctt taatccggcg atagagttct
     3061 agagagcgaa tagttatagc cacaattgca atcactgtga ttaggtaggc gctaagacca
     3121 aaaacacttc gcatcatgcg ccttagggta ggtgggcaag agccaaaccg caggtaaggg
     3181 ctctatacac aggggtggat aacctgtgga gaattacacg gatgtaatta aaaaagaggt
     3241 attagcgcca gcgagtagct agggagaagc ccactccgat gaaggcaaag cccacaacca
     3301 tgttccaagc cccgatattg ggtattgggt attgggtctg agagacatag aaaataacta
     3361 tccaaaacag gcctatcaag aatgcgccaa tcattgttgg ggcgagccag gctgggctct
     3421 ccatttcaac aggatctcgc tcagcaagaa gtatctctgg gcggttatct ttcttcttct
     3481 tacgcagtct ggatttaggc ataggcgtga ggatacagga gaggatgagg ccatgggaac
     3541 aaggatcttg gtggttgata actatgactc ctttgtcttt aacctcgttc aatatctcca
     3601 gcaactgggg gccgagtgca cggttgtaag aaatgatgaa gttagtgcaa gccttgctga
     3661 tcaatatgac ggggtcttaa tctccccagg gcctggaact cccgaagaag ctggcatctc
     3721 gatagcgatg attaagtatg cagctgagat aaacaagccg ttacttggag tttgcctagg
     3781 acatcaagga attggggttg cttatggagc aactgttaac cgagcgccag agctacttca
     3841 tggaaagaca tcggttatca ctcataacaa ttctggagtt ctaaaagata tccctaataa
     3901 ctttactgcc actcgctatc actcacttgc gatagatgaa aagagtttgc cagaggaaat
     3961 tgaaataact gctagaagtg agagtggctt agtaatggca atcagacata aaacacttcc
     4021 aattcatgga gtgcaatttc atcctgaatc agttcttacc gaacacggct atcagatgct
     4081 aggtaattgg ttagtagagt gcggtgatag tagtgctaga gagcgatctg agggcctctc
     4141 tccagttgtt gctcgctagc cttgagtttt attgatagta attgttactg aagagccaat
     4201 aagttttatc tctccagcac ttggcgcctg tgctaatacg cttccaatta cttgatttgc
     4261 atcatatgcc tcaacaattt ttactaagaa ccctgcttgg gtaagaattg ttctagcttc
     4321 gatttcgtta agtcctacta aagatggcac agagatattt ccactagcga tctctagaac
     4381 taccccagta cctgcttcaa ctgtgcttcc tggctctggg atgattttta aaacaattcc
     4441 aggctcttgc tctgaatcaa tagcgatagt tctactaact cttaatcctg cagcagcaag
     4501 tgctgccctt acctcttcaa gaggtttacc caataaacca gtaggaacaa cggcatctcc
     4561 tggcccatct gaaatcgtta acgtcactgc tgagccatca gagatacgag ttgtggcaag
     4621 tggtagttgg ctagcgactc gatcttttgg aattctagga tctggcgctc tttggatggt
     4681 gattgttagc cctgctaatt cttgacgcgc ctgcgcctct gttaaaccaa caacattagg
     4741 aagaaccctt gttactgcgc tctggtttgg attaaagatg aaataactaa cgccgccgac
     4801 aagaagtatt aaagcaattg aaagggcagc aactttactt cgcgccttct cttttcttaa
     4861 ctgttttgga atctgagtgg taattccttc acctttaatc aatttcttta aatcttttag
     4921 cattgcatta gcgctttgat atctctcatt tgcatcttta gctagagcga tactaaagaa
     4981 cgcgtttaaa gcaggatcaa gactttcttg aatttcagta attgggacaa gtggagcaga
     5041 gacatgttga taagcaatag caactggtgt gtccccagta aagggaggtc ttcctgccaa
     5101 tagttcataa agcaaacatc caacagagta aagatcgctt ctagcatctg cttgagttcc
     5161 agtggcttgt tctggcgaga gatactgcgc tgttccaatt atgttccaag tgctagtcat
     5221 agtagctcca gtatcagcaa gagctctagc gataccaaag tccattactt tcacatcacc
     5281 gttatctgtg atcatgatat ttcctggttt tatatcgcga tgaataattc cattcttatg
     5341 tgaatactgc agtgcgatta aaataccttc agctatttcg acagcgcgat ttaacgcaaa
     5401 acgctcacct ttatttacta tttctcgcaa ggttttaccc tctacatact ccataacaat
     5461 gtatggcata gccccacttg ggctatcttc tttaccggag tcgtaaaccg agacaatgcc
     5521 tgggtgattt agagcggcaa cggataaggc ttctttattg aaccgagtaa caaacgatgg
     5581 gtctctagct aaatcacttc ttaatatctt gatagcaact tggcgattaa gtcttacatc
     5641 ttgggctaga taaacatcag ccatcccacc agtgccaatc atggctccta attcatagcg
     5701 ctcaccaagt atttttttca tagcgcagcg ccaaaaccct gagttttaat gctggcctta
     5761 ctctcgccaa tttcagcaac tatcactgtg acattatctg gcgctccagc tttatatgta
     5821 agctcaatta atgaattcac agcacttatt aagtcttgtt gcatcgcttt agcaatctgt
     5881 gcttcatcaa gcacagcggt taggccatca ctacaaagca agtatctatc accttttaat
     5941 actggataag caagaagaat aggagagaga ttctctttac ccattagcgc ttgggttaga
     6001 aaagatctct gtgggtgatc tgcaatctca tctgggctaa gccttccttg atctattaat
     6061 tcttgaacca ccgtgtgatc atggcttaac tgttctattt tcttgccgcg caagcgatat
     6121 gcccttgaat ctccaaggtg caataaaagt agattgctat caaagagagc tattgaagta
     6181 agtgttgttc ccattcctac taactccggg cgcgcagctc caatctcagc aattgccgca
     6241 tccatacttt caatagagga gctaagaaga tcttccctgg attcattatc tagctctgga
     6301 ttttcaagag ttggcaggag ctgaattaac ttttcaatta cagtccttga tgcaatctcg
     6361 ccaccaacat aaccacccat gccatctgct actgcaagaa gagagttaga gactaaagct
     6421 gaatcttcat tgccttgacg ctgcaggcca acttcagatc gccatgcgat atcagagaag
     6481 ttcagtttta ccgccataat attgccaagc ctaacgcgca aatcgctatt gataaaagcg
     6541 tatgcgtcca agatggccct acgagtatcc ttacgcagtg agaagatacg catatctagg
     6601 gccagctgga actttcacag aagaagctct gcgcactatt gcagaaccaa gcgatgaatt
     6661 acacccttat gcaaatgtca ctgcagcgct taatgcggtt agaaactctc aagcagatca
     6721 agctctagtt cctattgaaa attcagttga aggtgttgtc gcaagaaccc ttgatgagtt
     6781 ggcaataggt gaacctttag tgatttatag agagattact cttcccgttt ccttctcact
     6841 tcttgtggct aaaggaaaag agagctctca ggtaaagaaa atcgctaccc atcctcatgc
     6901 tgaggcgcag tgccgaagtt ttattgcaaa gaattatcca gatgctgaaa ttattccaac
     6961 ttcatcaacg gctgcagctg cagcagattt agttaaaagc ggttttgatg cagcaattgc
     7021 tagccctgct gcagcaaaag aatatgggct aagtgcaatc gctaataaca ttggtgataa
     7081 cgatggcgct gttactcgct ttgtgttggc aggaaaacca ggattagtgc cagctctatc
     7141 aggccatgat cgaacatcgc tagttgcttt tattggcaca gatcatgctg gcgcgctatt
     7201 ggaggttttg acagagtttt ccatgcgcgg ggttaatctc acctttatcc aatctcgccc
     7261 aacaggcaaa gaattaggca gctatcactt tattattgat gtggaagggc atataaacga
     7321 agaacgcgta ggagatgcgc taactggcct tagaagaata tgtgaggatg tccgctatct
     7381 aggttcttat ccaagagcag ataaaattgc tccaacgaca actacaaaaa ctgccgataa
     7441 ctccttcaag caggcagatg cttggctttc agcagttcgc gccggagaga agatttagat
     7501 gattgatata aaggtattaa gagagagccc agatttagtt agagcatctc agagtgcccg
     7561 cggcgaagat gtatctcttg ttgatcgcgt acttgcagca gatgaaatta gacgaagtgc
     7621 gatagtggaa tttgaagcgt taaaggccga acagaacact ctttcaaaga gcgttggatc
     7681 tgcaaaaggc gaagagaagg cagcgctatt agaaaaagcc aaagctctat cagatgcagt
     7741 aaaagccgcc gagagcaaga aaaatgcaac agaggctgag tacaaaagta ttgctatgtc
     7801 tctttcaaat attgttgata aggcagcgcc agttggcggt gaggcagatt tcaaagtact
     7861 agaagaagtt ggcagaccgc gagagtttaa tttcactcca aaggatcatg ttgaacttgg
     7921 caagatatta ggagcaattg atgtagagcg agcagcaaaa gtctctggcg ctcgttttta
     7981 ttatttaacc ggagtaggag cgcttttgga attagcgctc gttaactacg caattaccag
     8041 tgctagtaaa gcaggcttca ttccagttat tcccccagtt ttagtcaagc ctgccgcaat
     8101 ggaggggact ggttttctag gtcaagccgc tgagaatgtc tttcacttaa aagatgagga
     8161 tttctattta gttggaacta gcgaagttgc tctagcagct ttacatatgg atgagatatt
     8221 agatgccaaa tctcttccaa ttagatatgc cggctactct ccatgctttc gtaaagaggc
     8281 aggatcttat ggaaaagaca caaggggaat tatcagagtt catcagttcg acaaggtgga
     8341 gatgttttca tattgcgctt tgcaggaagc agaggcagag catcaaaaat tattgaattg
     8401 ggagaaagac ttcttaaacg ctatggaaat tccttatcga gtaattgatg ttgcaacggg
     8461 ggatctgggc tctagcgcta atcgcaagtt tgattgtgaa gcttggattc caacccaaaa
     8521 cgcgtataga gaagtaacta gtacttctaa ttgcagtgat ttccaagcta gaagattaaa
     8581 tattcgaatt aagaccgagt ctgcaacaac tccagtagca actcttaatg gaacattagt
     8641 tgcaattcca agaatgattg ttgcaattct agaaaatcac caacaaagtg acggttcagt
     8701 actactgccc aaagctctgc aaggccttct tggtatggag cgattcactc cggtggctaa
     8761 ttgagtgcag taactcgtcg ccctaaatta attgcaacag atctagatgg caccatcgtt
     8821 gctcactacg ggaaaatctc agagcggacc aaagcagcct ttaatgcagc tcacaattta
     8881 ggtgttgaaa tattttatgt aaccggaaga cctccaagat ggatgccaga gattgcaaag
     8941 gtatttcctt ttggtcatgg gatttgtgct aatggcgcgc tgctctatga cattcataat
     9001 gagcgagtat tagaagagtg ggcaatgtca gttgatagtc agattgagac agtaaatcga
     9061 ttaagacgaa gtatccctga gatctcattt gccgttgagt ggcataacta ttttctccgc
     9121 gaaaagaaat acgtccctcg ctgggatgtt ggtcaggaca acgttggcgt tgatgacatt
     9181 acagagatta ttgatagccc agcaataaaa atgatggcta gatgctcaaa ccaagaactt
     9241 agctcggatc agatgttaag aattgcgcag ctagagttag agggagttgc cacagtgact
     9301 cactcaaatc ctcatgattc acttcttgaa ataaatgctc atggaatttc caagggagag
     9361 acactttcca agattgccgc cagatatggc ataagcgctg atgaaactgt ggcctttggc
     9421 gataatccga atgatttttc aatgcttgct tgggcttctc gctcttgggc tatggctgat
     9481 ggccatccag atgctgttaa atatgcaaag ttcgtggcag aaccacatga agaagatggt
     9541 gttgcaaaag aaatagagaa gttattggag ctacctgaat gaacacagcg ctagcagttc
     9601 cgctttggct cgatttacta accgttggaa ttggcgcgct tcaaggcgcg ctctttgcaa
     9661 ttttttataa acgttttgat cttgttgggg ttatttcagt agcaatactt acaggtcttg
     9721 gtggtggaat tttaagagat ttattacttg ccgttggtag acctgcagca atgcaagata
     9781 aatatattct cacagcaatt gcatcttgct tagtcgcaat tcttattggg cgttggtata
     9841 aaagagcaag tagcacagta gtctttcttg attcagtagc aatgtctctc tttgctgttg
     9901 ctggaactta taaagcaatt gttaatggca cctctgagtt agtcgcaata ttgcttgggg
     9961 tgttaactgc agtaggaggc ggagtacttc gcgatgtagt ttgcagaatc acgccacaga
    10021 tatttactgg aggcccgcta tatgccactg cctctgcggt aggagctacg ctatttgtat
    10081 tacttgagaa gagtgcaatt gatttaaatg ttgcccttgc agtatctgca ataacgattg
    10141 tggcaattca gatggcatca cttcgctttc gaattcacct aaagcccgtt ctagattcat
    10201 tggattttga agacccctct aagtgaaggt aagtttcccc acggagggct cgcatagtgg
    10261 cctagtgcac cggtcttgaa aaccggcaag ggaaaccttc gtgggttcaa atcccacgcc
    10321 ctccgccatc tactacgcct agcagctaac ttcttgtggg cgatttcata aggctctacc
    10381 tagcaggatt tagctaacac aaggaaaata agaaggtatt tcccaagaag ttttaaagga
    10441 ggcgctcagt ggctacagga gttttctcta caactcgagc cgcaataaag gagcgcactc
    10501 ttcgaactga tcgttggtgg ctacaaccac taattatcgt tgcagtctta atctcattta
    10561 ttatctatgc aacgtttcgc gcttttgaaa ataagtatta ctttgcagag cctctaatct
    10621 caccttttta ttcgccatgt ctttcaacag tatgcgttga aggagctgct cactttggta
    10681 cacctattgg cgcattcact atctttggct tcttaatttc acctgcgcta tttattttga
    10741 ttttcccact aggttttcgt ttaacttgtt attactaccg taagtcttac taccgctctt
    10801 tctggatgtc gccacctgca tgcgcagttg cagagcctca ctctaaatac acaggggaga
    10861 ccagagcgcc actcattttg caaaatggcc atagatggtt cttctatgca ggtttagttt
    10921 taaacgtaat tcttacctac gacgcaatca ttgcttttaa aaacccagag ggcgagtggg
    10981 gtcatatgag cgttggaact ttagtgctac tactaaatgc caccatgctc tggctctatt
    11041 cagcttcttg ccatacctgc agacacacaa ttggtgggcg actaagaaat ttctctaaac
    11101 atccaattcg atacaaatta tggagctggg tctcagtctt aaaccacagc catccaacat
    11161 atgcctggat atcactattt gttgtggcat ttactgactt ttatatccgc ctggttgcta
    11221 ctggcaccat aaccaattac tacttcttct aggccggcga tgaataacga tattaaattt
    11281 agtcgctata acttcgatgt agtcgttatt ggcgcaggcg gcgcaggtct tcgtgcagca
    11341 gtagaagccc gtcaagcagg ccttcgtgtt gcaatcattt gtaagtctct atttggtaag
    11401 gctcacaccg ttatggcaga gggcggcgca gctgctgcga tgggtaatgt gaatgacaat
    11461 gatggttgga aagttcactt tagagacaca atgcgcggtg gaaagttttt aaataactgg
    11521 agaatggctg aacttcacgc aaaagaatct ccagatcgca tctgggagct agagacctgg
    11581 ggcgcgttat ttgatcgaac taaagatggc cgtatttccc agcgaaactt tggtggccat
    11641 gaatatccac gccttgccca cgttggcgat agaactggtc ttgaattaat tagaacaatg
    11701 caacaaaaaa ttgtttcaat gcagcaagcc gatgccaaag agcatggcga tgccgagaag
    11761 tatttgaaag tatttgcaga gttaacaatt actgagatat taaaagatgg agagaaagtc
    11821 tctggttgct atggatattg gcgagagact ggcgatgaag ttttatttga agcccctgct
    11881 gttgttttag caacaggggg aatcggaaaa tctttcaaga ttacttccaa ttcatgggaa
    11941 ggaactggcg atggccacgc cctagccctt aaggcaggat ctgctttagt tgatatggag
    12001 tttgtgcaat tccatccaac cggaatggtt tggccaccat ctgttagagg aattctcgtt
    12061 actgaatctg ttcgtggaga tggtggagta cttaccaata gcgaaggtaa gcgctttatg
    12121 tttaattaca ttccagaagt tttcaaagac aaatatgctg ataatgaagc agaagctgat
    12181 cgctggtata aagaccaaga gaacaatcgt cgtcccccag agcttctgcc tagagatgaa
    12241 gtagcgcgcg caattaactc tgaagttaaa gcaggtcgcg gatctccaag aggcggggtt
    12301 taccttgatg tttcaaagcg cctgccagct gatgaaatta agcgtcgtct accttctatg
    12361 tggcatcaat ttaaagaact tgcagatgtt gatatcactg aacaacccat ggaagttggt
    12421 cctacctgtc actatgtaat gggaggagta aaggtagatc cagatactgc cgctgcttat
    12481 caagttccag gtttatttgc cgcaggtgag gttgcaggcg gaatgcatgg ctctaatcgt
    12541 cttggtggta attcactttc agatcttctt gtttttggaa gaagagcggg agcgggagct
    12601 gcagaatatg tgaagaagtt agcaagtaat aggccagcag ttagcgataa agaaattgct
    12661 agagctcata gccatctaaa tgaaccattt acccgtgatg gcaacgagaa tccttatgct
    12721 ctacatgatg agcttcagaa tgtgacccaa gatttagtag gaattattcg aaatgagaaa
    12781 gaactctctg atgctctcgt aaaacttgaa tccattcgaa aaagagcggc gcaggtcaaa
    12841 gcaagtggcg gaagagcatt taaccctggc ttccacttag ctttagattt agagaacatg
    12901 ttgttagttt ctgaatcaat tgctcgttca gcacttgcaa gacaagagtc aagaggtggc
    12961 catactcgtg atgattatcc aaagatggat gcgaattggc gcactatcaa tcatttgagt
    13021 aagtggaacg gcaagcaggt tgaaattgat cctgccaagt ctcagatgct tcctgctgag
    13081 ttaattgagg tatttgagat ggatgaacta aagaagtatt acactgaagc agagcttgcg
    13141 aagggtggca actaatgtct cgtaaagtaa agctacgtat ctggcgtggt gatgccaaag
    13201 aaggcaagct agaagatgtc agcgttgagg ccaatgaggg cgaagtagtc cttgatgtaa
    13261 ttcatcgagt tcaaggaaca cagatgccag atttagcagt tcgttggaat tgtaaagccg
    13321 gtaagtgtgg ctcttgctct atggaaatca atggaaaacc tcgtcttgcc tgcatgacgc
    13381 gtatgaatac atttgcagag gatgaaacga taactgtcac tccgcttaga gcttttccag
    13441 taatccgcga tttagttacc gatgtttcct ataactatag aaaagctatg gagattcctt
    13501 catttgcagc gccagaggat ttaaagcctg gggaataccg tatggctcaa gtcgatgtgg
    13561 agcgcagcca agagttccgt aaatgtattg aatgcttcct ctgccaagat acttgccatg
    13621 ttatccgcga ccatgaagag aataagaagt cttttgctgg tccacgcttc tttattcgta
    13681 tcgcagagct tgatatgcac ccacttgatc gacttcgtaa ccgtaaacaa aaagctcaag
    13741 aagagcatgg tcttggaatg tgtaacatca caaagtgctg cacagaagtt tgcccagaac
    13801 atatcaagat tactgataac gccatcattc caatgaagga gcgagtagtg gatgtgaaat
    13861 acgatcccat tcgctggctt ggttcaaaga ttcgcaagcg cgaagggata agttaatgaa
    13921 cctagtaaag cgttgggcga tacttgccct tgctttctgg ttaacaacat ttattgtctc
    13981 tggcatagag attcaagatg gtgcttggaa ttacttctgg gtagcggccc tctttggagt
    14041 tgtaaataca cttcttggtg gcctattaaa gctatttacc cttcctgcag tgatactaac
    14101 tttcggcctc tttgtatttg taattaatgc agcgatgttg accctggtag atcgctggag
    14161 cgatgtttta acaattgata aattcacatc agctctagta ggggctttaa ttattagcct
    14221 tatttcaggt tttacgaata aattaatcaa taaggcttaa acgctagcga attgaaacgt
    14281 ttactagcag ttggacttgg gggagcactt ggctccctgt ttcgctattt aacggtttca
    14341 aaaggcgatg aaataggact ctttttagta aatatatttg gtgtggcagt tgctgggttt
    14401 atcgccctac gcctatctac ctcagagtta attcgcctag ccttaattcc aggttttgct
    14461 gctggtctta ctaccttttc atcagttgca gtcattcatg ttgaggacaa ttcactacgc
    14521 agtatcgctt acttctttgg tagcgtctta gtgagccttt tgattctctt tgccataaaa
    14581 cctaaggtga aaatatgaga gttagctatt ttctactggg cgcatttgtt ggcgcacccg
    14641 ttagatttgt aattgttgaa tacttaaaga gctatctcaa attcccacta gggatattgc
    14701 tagttaatgt aattggctct tttttacttg ggttaatcct tgaatcagat accaacatag
    14761 cctttgcctt gatgggattc tcaggcgctc ttactacctg gtcggctttt gctatggact
    14821 tgtttgagca ggctaaaagg cgcgagcagt tgctcttttt attgaatctc tttggaaatt
    14881 atgctcttgc tcttcttgct gcaggccttg gcatctggat tgcgcaataa tatgaatatg
    14941 aaaaagagcg ctctatttct caccctctct ttattgctgg cctcttgtag tagccaggaa
    15001 gaggttgtcg ccccagagct accatcggtt ccaagtaact gcgcagatac aaaggtttta
    15061 gaatctatat tgccaagaat tgctgatgct aaatacattg aaacagagtg ggagccagca
    15121 gaaggcactg atttgtatgc tgcctataat gctggcggca ttgcttgcac ctatggcctg
    15181 caagaagctg aagttggagc aacaatttta tgggctccag ataacaagat tcttttttca
    15241 gagctaactc caaactggat tggatttggt caaaaagaga ttgatcttcc tgggatagaa
    15301 gaggaagctg cttattatct aagtgaaggc attgagggcc agggcgagta tcacatctgg
    15361 tcagttaact tattgataaa tggctcatgg attcaggttg gagcaacatt ttttaactca
    15421 ctggaggatg caataccagt aataaaagca gccatagatt cactacgaac tccaaagcgg
    15481 gctgaaaaag agaaaattat gggttgttat ttagcagagc tacctaatga tctttatgtt
    15541 tttaacgttc actatcacga taacaataca atctcggctg acttttatta taagaatata
    15601 aatggtgagc catctaaggg attatttctt ggaacctata ccaatggcat agcgcgaggt
    15661 ttctatagtt tacctaccag taatggaaca agtgagcggg agctatttct aaaaggtgat
    15721 aagaacggtt ttgtgacagc gactgcgaag ttagaaaaag taggaagtat tgaaaaatac
    15781 gtaagacctt tggatataaa atggagtcag gaaattaaat ttatccctgc tcaggaatgt
    15841 gaaggtctac ttagaggtta aagctgccat ccaggattca acttcatctg gatgagaagg
    15901 cattgcgcta cttaagtttt cacaaccatc tttagttacc aagacatcat cttcgattct
    15961 cactcctatt ccccgatact ctgcaggaaa tagcgtgtca tctggctgga tataaagtcc
    16021 tggttcaaca gttaaaatca ttccttcttt caaaacacct tcagtatgag cttcttttct
    16081 agcctttgca caatcatgaa catccattcc gagcatatgt cctgtgccat gcacggtcca
    16141 gcggcgatga agtcctacat ctggttttaa cgattcttgc gctggaattg gaagaactcc
    16201 catctcttca agccctctag caagaacttc gctagctgct atgtgaaagt cacgaaactt
    16261 tgcgccaggt ttgactgcct tcattccagc tttctgtgct tcatatacca aggtatagat
    16321 cttgcgctgg gctggagtaa acctgccatt tattggaaaa gttctagtga tatcggctgt
    16381 aaacaaagag tccatttcaa ctccagcatc aattagaagc agttcaccat cacgcagttg
    16441 tccatcattt ctaatccaat gaagaacgca agcatgcgaa ccagctgcca cgatactgtc
    16501 atagccgaga tcatttcctt cgaatctagc tcttgtgaaa aaagctcctt caattaatcg
    16561 ctcgccacgc tttttaccga ttgcacttgg aatttgttta atcatgtctt caaatccgcg
    16621 atgggtagca tcgatagctt ttcgcatctc agctatttca tattcatcct taactaagcg
    16681 tgcttcagat acccaggaag caagttcttc gtctccgcta ttttctctaa ctctagaatc
    16741 aatgtaatta tcttccccac gaataatcaa agtttctttc tcgcttgtca gaaaatcatc
    16801 aagggaatca atatggcgaa ccgttatgcc ataaaccagt tcactttcag ctagagtcat
    16861 tcttcgccca atccagaact ctccatgtct tgggtttcta taaaactcat cacttctctc
    16921 tcttggagat cttggatgaa taaatagaag actttcatgg cttgagccac ttggttctag
    16981 aatcaaaatt gaatcaggaa ctgcatcact tccaccaatc ccagtaagcc aagaaaaagc
    17041 ggtgtgaggc ctaaagcgat aatcactatc gttgcttcta actttaaatg tgccggcagg
    17101 aataacaagc cttttaccag gaaatgcttt agatagaagc tctctacgtt tggcagcaaa
    17161 gtctgcagca ttactctttt gtagattctc tagaggagag ggagcccatc cttgctccat
    17221 gaatttcatt aaaggctcag gagtagggat gtcgtgtggg cgtgagggaa gttttcttcc
    17281 cacctcttta tcgaatgtgc tcataaagta agagcgtaca ggcttgattc caagctttca
    17341 tcaagagtga gtaaactccc ctgataactg gagacgtcgc atagcccggt ctagtgcgcc
    17401 tcactgctaa tgaggttttc ccgaaaggga atcggaggtt caaatcctct cgtctccgcg
    17461 tggctgaatt tcgaatcgca ataatcggtg ctggccctgc cggctacttt gctgcccaag
    17521 ccttgcagag cgcagagatc gacgggaaaa catttgcgat agatatgatt gaaaaacttc
    17581 caacaccttg gggcttggtg cgaagcggtg tggctccaga tcatccaaag attaagagcg
    17641 tctctaaagt ctttgaaaaa attgccgccc aagagggttt taggttgatt ggaaatgttg
    17701 agctaggcag agatatttct ttaaaagaaa tagaagagtt gtatgacgca gtaataatcg
    17761 ctactggctc aacggtcgga agaaaacttg ggattccagg agaagagcta gcaaactcaa
    17821 tatcagccgc tgattttgtt ccttggtata acgcccatcc ggagtataaa aacttagagg
    17881 tagattttag taatcgggta gcagttgtta ttggcgcagg aaatgttgca attgatgtgg
    17941 caagaatgct agcccttaat ccaaaagagc tagacccaac agatactgct caatacgctc
    18001 tagataaatt acataactct gcaatcagag aggtacattt atttggaaga cgtggggccg
    18061 aacatgcggc ctttacctca cccgaacttc gagaactacc aaagcttgaa tacaccgatg
    18121 tttttattga tgaaaatgag ataagcgcag ctcaacagag agctagtagc gcaggtgaaa
    18181 taccaaaaga tgtaaaggca aatcttgagg cgatggctgc tatcgctacc gctgataaaa
    18241 agggagtaga tcgcaaactc tcgcttcact tcttagctac tccccttgaa ataaagggga
    18301 aggacagagt tgaggagatc acctttagcg ttaatgaggt caaagatggg caagtagttc
    18361 caaccggaaa gactcattca gttaagtgcg gattagttat tagcgctatt ggttatcgct
    18421 gtctagaact gccaggactt gtctatgaga gtggaaagat taaaaatacc gatggccgta
    18481 tcggttcgag caatacctat gttgttggct gggctaagag aggcccaaca ggcgtaattg
    18541 gaacaaataa gagtgattcc tcagaagtta ttaaattact tatctcgaat ctgacaactc
    18601 caaaaaatag tcgagatttt ctcgagatat taagctctag aaatatcacc tatatttcac
    18661 aaaaaggctg ggagcagata aataacgctg agatatcagc tggcgaagcc cagggaaaac
    18721 ctcgcgtgaa aattactgat atacaagaaa tgctaggctt cgcaggctaa ttgcgcccgt
    18781 agctcaacgg atagagcatc tgactacgga tcagaaggtt tggggttcga atccctacgg
    18841 gcgcgcaaga
//
LOCUS       DAOROD010000021        17010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_123_length_17010_cov_39.530404, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000021 DAOROD010000000
VERSION     DAOROD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 17010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17010
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_17010_cov_39.530404"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLW53_04845"
     CDS             complement(<1..1307)
                     /locus_tag="PLW53_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36781.1"
                     /translation="MSSCTLIPLARPTFDVSAAQKFFDSARDLLGEIGATVNGPTSLV
                     MTPEDTASAEANLKSDEKLYILFNASFADASAAVSLLSKVSGDVLLWSVREFGEIGDR
                     LLLNSMCGSNLAAHALRVNGKRITHLHGNPDEAHVRDALSSALAGKMPDVGQPQKVTG
                     QLADLDKVKAALSKLSGLTIGAIGDAPAGFTPCTYDEEALKKSFGLNIINKSIPEIFA
                     DIAAVPLDKESAEYKDACQAQPSLKNVPEKEARVNASTRVALDNWIRANDLSAIAMRC
                     WPDFAVDLGACPCGAMGRTATSGTPAACERDVYGAVTMLLLEALGSGTNYLVDTVDLE
                     EDENIIRIWHCGSASTTLAVDPDNATQFIHCNRKLGVAGNFPLKTGPVVLVRLDTDID
                     PANQSKLRLVMTSGESLSAPNRFQGNTATVRTSAPARQLINGL"
     gene            complement(1307..2413)
                     /locus_tag="PLW53_04850"
     CDS             complement(1307..2413)
                     /locus_tag="PLW53_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD36782.1"
                     /translation="MLRYGIIGMGAMGREHAENLSFIEGASVTAISDPDSGSRELAIK
                     QLGESVKVFTDHRQLLDSGLIDVVIIATPNFTHVDILEDALKTSLHVFIEKPLCTTIE
                     DCLKVIEWSENRSGLVWMGLEYRYMPPVAELISIVHSGGVGQVQQVSIREHREPFYPK
                     VGNWNRFTSKTGGTLVEKCCHYFNLMDHIANERPNRVFASGGQRVNYLDESYDGVKAD
                     MLDSAYVILEYPSGKRAMLDLCMFAENTVDKEHISVTGSEGKVESFLPSLELRYGKRS
                     AVGNFKQWSHDASKPKVESRKVWDESIKYNGFHYGASYLEHLKFFNAIKSGKGPDVSL
                     EDGLRSVASGLAAQMSIAQGRPVAMSEVLPAGWN"
     gene            complement(2532..3605)
                     /locus_tag="PLW53_04855"
     CDS             complement(2532..3605)
                     /locus_tag="PLW53_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRD36783.1"
                     /translation="MTRALEQREKRARIKALLESKGLDALILKKGANVAWIIGGRAHI
                     PTTLELACLDVIVYKDRIVVVTNKIEAPRLEAEELSGDEELIVINWFEGRDGQLPSGE
                     KIGIDGPDNNRINLSAEVEELRRSLNSFEVERLKEIGSDAAKSLGESMLKISPKMSEV
                     RAAGVIAENLWAHNLEPVVLLVAGSERIESFRHPLPTTSLIGSIFMGVICARRKGLIA
                     SVTRIVSFNSLSKEIEQRYQGLLNVEAAFFSGTKVGATYGQAFKSGEVEYLKQGFARD
                     EWSKHHQGGPTGYLPRDFPAHEKSSQIIGANNAIAWNPSAAGIKVEDTLVTTPTGFEI
                     ITSDPSWPSVEIAGRSRPDIARP"
     gene            complement(3615..4427)
                     /gene="map"
                     /locus_tag="PLW53_04860"
     CDS             complement(3615..4427)
                     /gene="map"
                     /locus_tag="PLW53_04860"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRD36784.1"
                     /translation="MAIQIKTLDQLKSMRQANLLVRSTLELVRANIKAGITTSALDAI
                     AEANIKRGGGISNFKGYHGYPAVICVSVNDEIVHGIPGDRMLMPGDIVSVDCGAIIDG
                     WHGDAAFTMIVDPIDSEHAKMLEVCEESLWHGIAAGVLGAKISDIGHAIEKYINSQGK
                     YGILREYGGHGIGNAMHMEPHILNFGKAGHGPTIEAGMVFAIEPMITLGSEKTKVLDD
                     NWTVVTQDKSYGAHFENSYCIAPDGKPFVLSVEDGGKAQLARFGIEVSSLLS"
     gene            complement(4431..4991)
                     /locus_tag="PLW53_04865"
     CDS             complement(4431..4991)
                     /locus_tag="PLW53_04865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRD36785.1"
                     /translation="MRLILVGPPGAGKGTQAVHLAEHYKIPHISTGDIFRANLQNGTA
                     LGLKAKAFMDKGELVPDSLTNEMVKDRLAKDDAKSGFLLDGFPRNVSQAEVLNAMLAE
                     SGVLLDAALELSLDNEEIVKRLSIRGRDDDKEEIIRRRLEVYSEQTAPIISFYRDANL
                     LVTIAATGEISDITSAAIAALSKKLS"
     gene            complement(4991..6289)
                     /gene="secY"
                     /locus_tag="PLW53_04870"
     CDS             complement(4991..6289)
                     /gene="secY"
                     /locus_tag="PLW53_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRD36786.1"
                     /translation="MLLSVFAKAFKTPDLRRKIFFTLGIMALFRFGSVIPTPGVSYGA
                     VQRCLANVDTGGLFGLINLFSGGALLQLSVFALGIMPYITASIIVQLLTVVIPRFETL
                     KKEGQSGTAKLTQYTRYLTIGLAVMQTTGLIAVARTPGRLINGCTEAIIPDTSWQRIV
                     TMIFVMTAGTSVIMWLGELITDRGVGNGMSILIFTSIAASFPGQLWSIKLSRGWATFL
                     FIMAVGVLIVAAVVFVEQSQRRIPVQYAKKQVGRRQYGGTTTYIPIKVNQSGVIPVIF
                     ASSLLYIPSLVANFTNSQAPWAVWISTNFVQGDHPIYISLYALLIIFFAYFYVAITFN
                     PEETAENMRKYGGFIPGIRAGKPTSDYLAYVLNRITAPGSLYLALVAIIPIIALVLFG
                     ASQNFPFGGTAILIVVGVGLDTAKQIESQLQQRSYEGFLR"
     gene            complement(6323..6763)
                     /gene="rplO"
                     /locus_tag="PLW53_04875"
     CDS             complement(6323..6763)
                     /gene="rplO"
                     /locus_tag="PLW53_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRD36787.1"
                     /translation="MVLKAHHLRPAPGAKKAKTRKGRGEGSKGKTAGRGGKGTRARNV
                     VRAGFEGGAMPLVQRLPKLRGFKNPETVTYQAVNVSTINALFPKGGEVSIDDLYKLGA
                     ARKGRPVKVLGNGDISVKVNVTVHSFSSSASEKISAAGGSIKTL"
     gene            complement(6766..6948)
                     /gene="rpmD"
                     /locus_tag="PLW53_04880"
     CDS             complement(6766..6948)
                     /gene="rpmD"
                     /locus_tag="PLW53_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRD36788.1"
                     /translation="MPRLKVTQVRSKVGNPPDQRNTLRSLGLKRIGDVVVKEDRPEIR
                     GMVHSVRHLIKVEEVE"
     gene            complement(6950..7558)
                     /gene="rpsE"
                     /locus_tag="PLW53_04885"
     CDS             complement(6950..7558)
                     /gene="rpsE"
                     /locus_tag="PLW53_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006304483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRD36789.1"
                     /translation="MADKEVVSTEAEKPKRGRDKRERRDDRQSEKSPYIERVVFINRV
                     AKVVSGGRRFSFTALVVLGDGNGMVGVGYGKAKEVPQAIAKAVEEAKKSFFKVPRIQG
                     TIPHPVQGEDAAGVVLLRPAAPGTGVIAGGPVRAVLECAGISDVLTKSLGSNNPINMV
                     HATAKALKMLEDPAAIAARRGLPLERVAPAAIIRAMSATAGA"
     gene            complement(7558..7935)
                     /gene="rplR"
                     /locus_tag="PLW53_04890"
     CDS             complement(7558..7935)
                     /gene="rplR"
                     /locus_tag="PLW53_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRD36790.1"
                     /translation="MNISKKIVKGSTQVARARRHFRVRKRVTGTSERPRLVVTRSARH
                     LVAQIVDDSRGHTLASASTMEKELRATSADKTKKARSVGQLVADRAKAAGVTTVVFDR
                     GGNRYTGRVAALAEGARENGLDF"
     gene            complement(7932..8474)
                     /gene="rplF"
                     /locus_tag="PLW53_04895"
     CDS             complement(7932..8474)
                     /gene="rplF"
                     /locus_tag="PLW53_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRD36791.1"
                     /translation="MSRIGKMPVAIPSGVEVNISGQSVSIKGPKGTLSHVLPELITPK
                     KEESSVVLTRANDERAAKSLHGLSRTLISNMVTGVTAGYSKKIMIVGVGYKVAAKGKD
                     LEFALGYSHPINFAAPAGITFTVESPTDLIVSGVDKQLVGEVVAKIQKLRKADPYKGK
                     GLHLQGARIRRKAGKTGKKA"
     gene            complement(8480..8884)
                     /gene="rpsH"
                     /locus_tag="PLW53_04900"
     CDS             complement(8480..8884)
                     /gene="rpsH"
                     /locus_tag="PLW53_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRD36792.1"
                     /translation="MTMTDPIADMLSRLRNANMAYHEVVSMPSSSVKVRIAEILQREG
                     YIGGYKVSDNPEGFGKTLTLDLKYGSNRERSIAGLRRVSKPGLRVYAKSTGLPKVLGG
                     LGVAIISTSQGLLTDRQANKNGVGGEVLAYVW"
     gene            complement(8946..9131)
                     /locus_tag="PLW53_04905"
     CDS             complement(8946..9131)
                     /locus_tag="PLW53_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRD36793.1"
                     /translation="MAKTSLKVKASRKPKFKVRGYTRCQRCGRPHSVYQKFGICRVCF
                     REMAHRGELPGITKASW"
     gene            complement(9135..9701)
                     /gene="rplE"
                     /locus_tag="PLW53_04910"
     CDS             complement(9135..9701)
                     /gene="rplE"
                     /locus_tag="PLW53_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRD36794.1"
                     /translation="MSTVIAPRLKEIYKSEVAPALQSEFNFSNRMQIPTLSKIVVNMG
                     VGEAAKDSKLIEGAIRDLAAITGQRPLVTKARKSIANFKLREGQPIGAHVTLRGDRMW
                     EFMDRLLTVSLPRIRDFRGLSPKQFDGKGNYTFGLTEQVVFLEIEQDKLDRVRGMDIT
                     IVTSAKNDNEGRALLKALGFPFKDQGEK"
     gene            complement(9702..10016)
                     /gene="rplX"
                     /locus_tag="PLW53_04915"
     CDS             complement(9702..10016)
                     /gene="rplX"
                     /locus_tag="PLW53_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRD36795.1"
                     /translation="MRIKKGDTVLVIAGKDKGVTGKVLAVNTVTARVTVEGVNRVKKH
                     TKEDTSQRGAKVGGILTIEAPIAYSNVMLLDDDNKATRIAVRKNEEGKNIRISRRTGG
                     DL"
     gene            complement(10016..10384)
                     /gene="rplN"
                     /locus_tag="PLW53_04920"
     CDS             complement(10016..10384)
                     /gene="rplN"
                     /locus_tag="PLW53_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRD36796.1"
                     /translation="MIQAESRLRVADNTGARELLCIRVLGGTQRRYASIGDVIVCSVK
                     DAIPGGQVKKGEVVKAVIVRTVKENRRADGSYIKFDENAAVIIKEDNEPRGTRIFGPV
                     ARELRDKRFMKIISLAPEVL"
     gene            complement(10387..10647)
                     /gene="rpsQ"
                     /locus_tag="PLW53_04925"
     CDS             complement(10387..10647)
                     /gene="rpsQ"
                     /locus_tag="PLW53_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005872739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRD36797.1"
                     /translation="MSEDRKFRKTREGVVVSDKMDKTVVVKVEDRAAHGLYSKVITKN
                     KKFKAHDEDNTAGVGDRVRIMETRPLSATKRWRVVEIIEKAK"
     gene            complement(10644..10862)
                     /gene="rpmC"
                     /locus_tag="PLW53_04930"
     CDS             complement(10644..10862)
                     /gene="rpmC"
                     /locus_tag="PLW53_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRD36798.1"
                     /translation="MAKMITTEELRALSTEDLAEKLKDSKEELFNLRFQAATGQLESH
                     GRLTAVRKEIAKIYTVIRERELGIGGAA"
     gene            complement(10865..11278)
                     /gene="rplP"
                     /locus_tag="PLW53_04935"
     CDS             complement(10865..11278)
                     /gene="rplP"
                     /locus_tag="PLW53_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRD36799.1"
                     /translation="MLIPRRVKHRKQHRPKRSGNAKGGTAVAFGDFGLQATEGGYVTN
                     RQIEAARIAMTRHIKRGGKVWINIYPDRPLTKKPAETRMGSGKGSPEFWIANVKPGRV
                     LFEISGVTLDVANQALSRAAHKLPVKSRVVIREEV"
     gene            complement(11278..12096)
                     /gene="rpsC"
                     /locus_tag="PLW53_04940"
     CDS             complement(11278..12096)
                     /gene="rpsC"
                     /locus_tag="PLW53_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRD36800.1"
                     /translation="MGQKINPHGFRLGITTEYKSRWYAEKLYKDYVKEDVMIRRMMEK
                     NMERAGVSRIEIERTRERVRIDLHTARPGIVIGRRGTEADRLRLDLEKLTGKQVQLNI
                     LEVKNPEIDAQLVARGVAEQLAARVSFRRAMRKAMQTAFKAGAKGVRIQCGGRLGGAE
                     MSRSEFYREGRVPLHTLRADIDYGFAEAHTTFGRLGVKVWIYKGEVIASRTEREAAAL
                     AQVQAAKAERAPKRAPRAPKGEVTKTSVAERANEAAAAVESEVSPEVKSEGSDA"
     gene            complement(12271..12630)
                     /gene="rplV"
                     /locus_tag="PLW53_04945"
                     /pseudo
     CDS             complement(12271..12630)
                     /gene="rplV"
                     /locus_tag="PLW53_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(12620..12907)
                     /gene="rpsS"
                     /locus_tag="PLW53_04950"
     CDS             complement(12620..12907)
                     /gene="rpsS"
                     /locus_tag="PLW53_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006823225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRD36801.1"
                     /translation="MPRSLKKGPFVDLHLEKKVDEQNAKNTKTVIKTWSRRSMITPSM
                     IGHTIAVHDGRKHVPVYVTEAMIGHKLGEFAPTRTFRGHVRQEDRTVTRAE"
     gene            complement(12924..13757)
                     /gene="rplB"
                     /locus_tag="PLW53_04955"
     CDS             complement(12924..13757)
                     /gene="rplB"
                     /locus_tag="PLW53_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRD36802.1"
                     /translation="MALRKLRPVTPSSRGQSVSDFAEITRSTPEKSLIRPINSRGGRN
                     ASGKITTRHQGGGHKRAYRVIDFLRNDKDGVPAVIAQIEYDPNRSARIALVHFADGEK
                     RYIIAPLGVEQGTKIENGPTADIKPGNNLPLRNIPVGTMIHAIETRPGGGARIARSAG
                     ASVQLVAKDGPYAQLRMPSGEIRNVDVRCRATIGVVGNAEQSNINHGKAGRMRWKGRR
                     PTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEARTRKKKSSDQLIIRRRPANKS
                     R"
     gene            complement(13795..14088)
                     /gene="rplW"
                     /locus_tag="PLW53_04960"
     CDS             complement(13795..14088)
                     /gene="rplW"
                     /locus_tag="PLW53_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRD36803.1"
                     /translation="MRDHREVLIAPVVSEKSYGMLDENKYTFIVAPDANKTEIKIAVE
                     KIFKVRVLKVNTMNREGKSKITRYGVGKRSDTKRAIVQIAAGDRIDIFGGPVS"
     gene            complement(14085..14765)
                     /gene="rplD"
                     /locus_tag="PLW53_04965"
     CDS             complement(14085..14765)
                     /gene="rplD"
                     /locus_tag="PLW53_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRD36804.1"
                     /translation="MSLTIEIKDAKGAKAGTFDLPAEIFDVQVNVPLIHQVVTAQLNA
                     ARAGTAKAKNRGEVSGGGKKPFKQKGTGRARQGSTRSPNQRHGGVAQGPVPRKYDERT
                     PKKMIKAALSGVLSDRARNERIHIVSTIVESVNSKAAIAAVRQFSDRRRVLVVLAHGE
                     EASWRSLRNEEDLHLIRQDQLNAYDVVVADDLVFSKKAITDFVAGPSKGKSVTARAFE
                     SELEEALA"
     gene            complement(14768..15460)
                     /gene="rplC"
                     /locus_tag="PLW53_04970"
     CDS             complement(14768..15460)
                     /gene="rplC"
                     /locus_tag="PLW53_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRD36805.1"
                     /translation="MTTTQSKGSAIKGILGVKLGMTQVFDENSQAIPVTVVQAGPCVV
                     TQIRTLEKDGYEAVQLAFGAIDPRKVSKPESGQFAKAGVTPRKFVAELRTASASSYTV
                     GQEVKADIFTAGEIVDAQGTSLGKGSAGVMKRHNFRGFGDGHGVERKHRTSGSIGNRT
                     TPGRVFKGKRMMGRMGVETVTVQNLTVQAVDVEKSLILIKGAIPGRTGAQVFIHSASK
                     KALSEVINKAGA"
     gene            complement(15477..15785)
                     /gene="rpsJ"
                     /locus_tag="PLW53_04975"
     CDS             complement(15477..15785)
                     /gene="rpsJ"
                     /locus_tag="PLW53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRD36806.1"
                     /translation="MAGQKIRIRLKAYDHEVIDSSAKKIVETVERTGATVAGPVPLPT
                     EKNTYCVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            complement(15869..>17010)
                     /gene="tuf"
                     /locus_tag="PLW53_04980"
     CDS             complement(15869..>17010)
                     /gene="tuf"
                     /locus_tag="PLW53_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRD36807.1"
                     /translation="GHIDHGKTTLTAAISKVLHDKYPNLNEVSAFDQIDKAPEERARG
                     ITISIAHIEYQTEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTKE
                     HVLLARQVGVPSIVVALNKSDMVDDEDILGLVEEEVRDLLTKYEFPGKDTPIVRISAL
                     KALEGDAAWGEKIMELMNAVDTFIPQPVREVDKPFLMPVEDVFTITGRGTVVTGRIER
                     GIVKVNEEVEIVGIRPDSQKTTITGVEMFRKLLDEGQAGENVGLLLRGLKREDVERGQ
                     VVCKPGSVTPHTDFEASSYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPSGTE
                     MVMPGDNTAMTVTLIQPVAMEEGLRFAIREGGRTVGAGRVTKIVK"
BASE COUNT         4394 a         4215 c         3584 g         4817 t
ORIGIN      
        1 attaacccat taatcaattg ccgtgctggc gcagatgtac gaactgttgc ggtattacct
       61 tggaaccgat taggagcaga tagtgattca ccactagtca ttactaatcg gagtttgctc
      121 tgatttgctg gatcgatatc ggtatccagc cttaccagaa caaccggacc ggtcttcaag
      181 gggaagttcc cggccacacc tagctttcta ttgcagtgta tgaactgcgt tgcattatct
      241 ggatcaacag caagtgtggt tgaagcagaa ccacaatgcc agattcgaat aatgttctca
      301 tcttcttcta aatccacggt atctactaag taatttgttc cagagccaag cgcctctaaa
      361 agcagcatag ttactgcgcc atagacatcg cgctcgcagg ctgctggagt tccactcgtt
      421 gctgttcttc ccatcgctcc gcaagggcaa gctcccaaat caactgcaaa atcaggccaa
      481 cagcgcatag caattgccga taaatcatta gctcttatcc aattatctag cgcaacccta
      541 gtgctggcat taactcgagc ctctttctca ggaacatttt taagagatgg ctgagcctgg
      601 caagcatcct tatattctgc gctctcttta tcaagtggca cagctgcaat atctgcaaag
      661 atttcaggaa ttgatttatt aataatattt aagccaaatg acttcttaag cgcctcttca
      721 tcgtaggtgc agggagtaaa accagctggc gcatcaccaa ttgccccaat cgttaaacca
      781 ctcaacttag aaagggctgc tttaacctta tctaaatctg ctaattgacc agttactttc
      841 tgcggttgac caacatctgg catctttcca gcaagagcag atgagagagc atctctaaca
      901 tgcgcctcat caggatttcc atgtagatga gtaattctct ttccatttac tcgaagtgca
      961 tgggctgcaa gatttgatcc acacattgaa ttgagtagaa gtcgatcccc tatctcgcca
     1021 aactctctaa ccgaccagag aagcacatca cctgaaactt tagaaagaag agatacagct
     1081 gcagaggcat cagcaaaaga tgcattaaat agaatgtata acttctcatc agattttaga
     1141 tttgcctcag cgctagcggt gtcctctgga gtcattacaa gtgatgttgg gccatttacg
     1201 gtagcgccaa tttcacccaa aagatcacga gcagaatcaa agaacttctg agcagctgag
     1261 acatcaaatg taggacgagc aagaggaatg agagtgcagc tagacattaa ttccaccccg
     1321 ctggcaatac ttcactcatt gctaccgggc gcccctgggc aatactcatc tgagccgcaa
     1381 gacctgatgc aacgcttcta aggccatcct ccaggctcac atctgggcct tttccgctct
     1441 taatcgcgtt aaagaacttt aagtgttcaa gatatgaagc cccatagtga aatccattgt
     1501 atttaatgct ctcatcccaa acttttcttg attcaacttt tggctttgaa gcatcatggc
     1561 tccactgctt aaagtttcca actgcgcttc tcttgccata tcgcagctca agtgatggta
     1621 gaaatgattc gactttgccc tcgcttcctg ttactgagat atgttcttta tcaacggtat
     1681 tttcagcaaa catacagaga tctagcatcg ctctttttcc acttggatat tccaaaatca
     1741 cataagcgct atcgagcata tctgccttaa ctccgtcata gctctcatcg aggtaattaa
     1801 ctctctgccc accggaggca aatactcgat ttgggcgctc attagcgatg tgatccatga
     1861 gattgaagta atggcagcac ttctcaacga gagtgccacc agttttagat gtgaatcgat
     1921 tccaattgcc aacttttgga tagaaaggtt cgcgatgttc tctgatagag acttgttgaa
     1981 cttgcccaac tcccccacta tggacaatag agatcaactc cgcaactggc ggcatgtaac
     2041 gatattcaag gcccatccaa actaagccac ttctattttc tgaccactca atgactttta
     2101 aacaatcttc aattgtggtg cagagtggtt tttcaataaa gacatgaagt gatgtcttaa
     2161 gcgcatcttc caaaatatca acgtgagtaa agtttggggt tgcaatgatt actacatcta
     2221 ttaagccgga atctagaagc tgcctatgat cggtaaatac ttttactgac tctcctagct
     2281 gcttaattgc tagttctctg gaacctgaat ctggatcgct gattgcagta actgatgcgc
     2341 cctcgataaa agataagttt tcggcatgct cacggcccat ggcgcccatg ccgataattc
     2401 cgtagcggag catctaaata agacttcctt ccggccgtta ttgaagtggt cagaccacct
     2461 caattgcctg agtcttatgc cctaaacgag ctcggtcaac gcttttctac ctctttttta
     2521 taacgtttag gtcaagggcg agcgatatct gggcgggacc tgccagctat ttcaacgctg
     2581 ggccagcttg gatcagaggt aataatctca aagccggtag gggtagtaac caaggtgtct
     2641 tcaaccttaa tcccggcagc acttgggttc caggcaatgg cgttattagc accaattatt
     2701 tgagagctct tttcatgagc ggggaaatct cgcggtagat atccagttgg accaccttga
     2761 tgatgcttac tccattcatc tcttgcaaat ccttgcttta gatattcaac ctcaccagat
     2821 ttgaaagctt ggccatatgt agccccaacc tttgtaccgc taaaaaatgc tgcttcaaca
     2881 tttagaagtc cctgatatct ctgctctatc tctttagaaa gagaattaaa tgaaacaatt
     2941 ctcgtcacgc tagcaattaa accttttctt ctagcacata taactcccat aaagatagag
     3001 ccgataagac tggtagttgg cagtggatgt ctaaaacttt caatacgctc actaccagca
     3061 actaataaaa ccactggctc taaattatgt gcccaaagat tttctgcaat cactccagct
     3121 gccctaacct cgctcatctt tggtgagatc ttgagcatcg attcacctaa agatttagca
     3181 gcatcgctac caatttcctt taggcgctca acttcaaagg aattaagaga tcttcttaac
     3241 tcttcaacct cagccgataa attaatgcga ttattgtcag gaccatcaat accaatcttt
     3301 tcacctgatg gcagctgccc atccctgcct tcaaaccaat taatgacaat taactcttca
     3361 tcacctgata actcttctgc ctcaagtctt ggcgcctcta tcttgttagt tacaacaacg
     3421 attcgatcct tataaacaat tacatcaaga caagctagtt caagagttgt tggaatatga
     3481 gctctgccgc caataatcca agcaacattg gctcccttct ttaagatcag agcatcaagg
     3541 ccctttgatt caagtaaagc cttgattctg gctcgctttt cgcgttgctc taaggctcta
     3601 gtcataggga taacttatga aagaagcgac gaaacttcta tgccgaatcg ggcaagctgg
     3661 gctttcccgc catcttcaac agagaggaca aatggctttc catctggggc tatgcagtaa
     3721 gagttttcaa agtgagcgcc ataggactta tcttgcgtta caacagtcca gttatcatca
     3781 aggactttag ttttttcaga accaagagta atcatcggct caatagcaaa gaccattcca
     3841 gcctcaatcg ttggaccatg gccagcttta ccaaagttga gaatatgcgg ctccatatgc
     3901 attgcatttc caatgccatg gccaccatat tctcgcaaaa ttccatactt tccttgcgag
     3961 ttaatatatt tctcaatcgc atggccaata tcggaaatct ttgctcccaa aactccggca
     4021 gcaattccat gccagagtga ttcttcgcaa acttccaaca tcttggcatg ctcactatca
     4081 attggatcaa caatcatggt aaatgctgca tcgccgtgcc agccatcaat aattgctccg
     4141 caatcaactg agacaatatc tccaggcata agcatgcgat caccaggaat tccatgaaca
     4201 atttcatcat ttactgaaac acaaattact gctggatagc cgtgatagcc cttaaaattt
     4261 gaaattcccc cgccgcgttt gatatttgcc tcagcaattg catcgagtgc gctagttgta
     4321 atacctgctt tgatattagc tcttactaac tctaaagtgc ttcgcaccaa gagatttgcc
     4381 tggcgcatac tctttagttg atccaaggtc tttatctgga tcgccattac ttaactcagt
     4441 ttcttactaa gagctgctat ggcagctgag gtgatatctg aaatctcacc agttgctgca
     4501 atagttacca agagattggc atctcgataa aagctaataa ttggcgcggt ttgctcgcta
     4561 taaacctcaa ggcgccttct aattatctct tccttgtcat catcgcgccc tctaatggag
     4621 aggcgcttaa cgatctcctc gttatcaaga gataattcaa gtgcggcatc aagtaagacg
     4681 ccagattcag caagcatcgc atttaatact tctgcttgag agacatttct tggaaaacca
     4741 tctagcaaga agccagattt tgcatcatct tttgccagac gatctttaac catctcatta
     4801 gttagtgaat ctggaactag ctcgccttta tccataaaag cctttgcttt aaggccaagg
     4861 gcggtgccat tttgtaaatt tgcacggaaa atatcgccag ttgaaatgtg tggaattttg
     4921 taatgctcag caagatgaac tgcctgagtt cctttaccgg cacctggtgg tccaaccagg
     4981 attaagcgca ttagcgcaag aacccctcat aagagcgttg ctgcagctgg ctctcgatct
     5041 gcttagcagt atcaagacca acaccaacca cgatcaaaat tgcagttcca ccaaatggga
     5101 agttctgaga tgcgccaaag agcaccagtg caatgattgg aataattgca acaagtgcta
     5161 gatagagcga accaggagca gtaattctat ttaatacata tgcaagataa tcacttgttg
     5221 gctttccagc acgaatgcct ggaataaagc cgccatactt acgcatattc tctgcagtct
     5281 cttctggatt aaaagtaatc gctacataga agtaggcaaa gaagataatt agtaaggcat
     5341 aaagactgat ataaatcggg tgatcgcctt gaacaaagtt agttgaaatc caaaccgccc
     5401 atggcgcttg ggagttggtg aagttagcaa ccagagatgg aatataaaga agtgatgaag
     5461 caaagataac tgggataact cctgattgat taacctttat cggaatataa gttgtagttc
     5521 caccatattg gcgtcttcca acctgcttct tggcatattg aactggaatt ctgcgctgag
     5581 attgttcaac aaataccacc gccgccacaa tcaagactcc taccgccata ataaatagga
     5641 atgttgccca gccgcgtgag agcttaattg accagagctg accaggaaat gaagccgcaa
     5701 tcgaggtaaa gattaagatt gacattccat taccaacgcc gcgatctgta attaactcac
     5761 ctagccacat aattaccgat gttcctgcag tcattacaaa gatcatggtg acgatacgct
     5821 gccaagaagt atcagggatg atcgcttcag tacagccatt gattaaacga cctggagttc
     5881 tagcaactgc aatcaaacct gtggtctgca taactgcaag accgatagtt agatagcggg
     5941 tgtattgcgt taacttagcc gttcctgatt ggccttcctt ctttagagtt tcaaagcgag
     6001 gaataacaac agttaatagc tgaacgataa ttgaagcggt gatgtaaggc atgatgccga
     6061 gagcaaatac tgagagctgt aatagcgccc cgccgctaaa taagttaatc agtccaaata
     6121 ggccgccagt atcaacattt gctaagcagc gctgaaccgc gccataggaa acacctggag
     6181 ttggaataac agaaccaaag cgaaatagag ccatgattcc aagagtgaag aagattttac
     6241 ggcgcagatc aggagttttg aaagccttag caaaaactga tagcaacatt atcttctcct
     6301 cttactttca actagatatt atctagagcg tcttaatact tccgccggcg gcagagattt
     6361 tctctgatgc cgatgatgag aagctatgga ctgtgacatt taccttaacg gagatatcgc
     6421 catttcctag gaccttaact gggcggccct tacgagcagc gcctaattta taaagatcat
     6481 cgatggaaac ctctccacct tttggaaata gcgcattgat agttgagaca ttaacagcct
     6541 gataagtaac ggtctcaggg tttttaaagc cacgaagttt tggcaagcgc tgcacaagag
     6601 gcatcgctcc accttcaaaa cctgcgcgaa ctacatttct agccctagtt cctttaccac
     6661 cacgaccagc agtctttccc ttggagcctt cgcctcgacc tttacgggtc tttgcttttt
     6721 tagcaccagg agcgggacgg agatgatgag cttttagcac catagttatt cgacctcctc
     6781 aaccttaatc aaatgacgaa cgctatgaac cattccgcga atctctggac gatcttcctt
     6841 aactacaaca tcaccgattc tcttaaggcc cagtgagcga agagtgtttc gttgatcagg
     6901 aggattgcca acttttgagc gaacttgagt aacttttaga cgtggcattt tatgcacctg
     6961 ccgttgcgct catagcacgg atgattgcag ctggagcaac gcgctcaagt ggaagtccgc
     7021 gacgtgcagc aattgctgct ggatcttcca acatctttag cgcttttgct gtggcatgaa
     7081 ccatgttaat tggattatta gatccaagtg acttagttag aacatcacta attcctgcgc
     7141 actcaagaac tgcgcgcacc ggaccaccag cgataactcc agtaccagga gctgcagggc
     7201 gaagtagtac tactccggct gcatcttcgc cttgaactgg gtgcgggata gtgccttgaa
     7261 ttcttggaac tttgaagaat gatttcttcg cctcttcaac agctttagca atggcttgtg
     7321 gaacttcttt tgcttttcca taaccaacgc caaccattcc attgccatca cctagaacaa
     7381 caagtgcggt gaatgagaaa cgacgacctc cagagacaac ttttgctacg cggttgataa
     7441 agaccacgcg ctcgatatag ggagacttct cagattggcg atcatcgcga cgttcgcgct
     7501 tatcgcgtcc gcgctttggc ttctctgcct cagtacttac tacttcttta tcggccatta
     7561 gaagtccaac ccattctctc tagcaccttc ggcaagcgct gctactcgcc cggtgtaacg
     7621 atttccacca cggtcaaaga ccactgtggt aactccagct gcttttgcgc gatctgcaac
     7681 taattggcca acgcttcttg ccttcttagt cttatctgca gatgttgctc ttagctcttt
     7741 ttccatagtt gaagcagagg ccagagtatg gccacgtgaa tcatcaacga tttgcgcaac
     7801 taagtggcga gcagagcgag taacaactaa gcgtggacgc tcagatgttc cggtaactct
     7861 ctttcgcact ctaaagtggc gacgggcgcg ggcaacttga gtagagccct taacaatctt
     7921 cttagagata ttcatgcctt cttacctgtc tttccagctt tgcggcgaat gcgcgcacct
     7981 tgtagatgca gacccttgcc tttatatgga tcggctttgc gaagtttttg aatcttggca
     8041 acaacttctc caactagctg cttatcaacg cctgaaacga ttagatcagt aggtgattca
     8101 acagtgaagg taattccggc aggagctgcg aaattaattg ggtgtgagta gccaagtgca
     8161 aactctagat ctttgccctt tgccgctact ttatatccaa ctccaacaat cataattttc
     8221 tttgagtaac cagctgtaac gccggtgacc atatttgaaa tcagagtgcg agaaagacca
     8281 tgaaggctct ttgctgcgcg ctcatcatta gcgcgtgtta gtactactga gctctcctct
     8341 ttcttaggag taattaactc aggaagcaca tggcttagcg ttccctttgg tcctttaatt
     8401 gagactgatt gaccagagat atttacctca acccctgaag gaatggccac tggcattttt
     8461 ccgatacgtg acatagcaat caccatacgt aagcgagaac ttctccgcct acgccattct
     8521 tgttagcttg tcgatcagtg agtaatcctt gtgatgttga aataattgcc acaccaagtc
     8581 cgcctaatac tttaggaagt ccagttgatt tggcataaac gcgaagacct ggctttgaaa
     8641 cgcggcgcag accagcaatt gatcgctcac gatttgaacc atatttaagg tcaagagtta
     8701 aggtttttcc aaaaccttca gggttatcgc tgactttata gccaccgata tatccctctc
     8761 tctgcaaaat ttcggcaata cgaaccttca ccgatgaaga tggcatagaa accacttcat
     8821 ggtaggccat attggcgtta cgcaaacgag agagcatgtc tgcgatcggg tcggtcattg
     8881 tcatagttgg ctcatcgcct ttctttggct ggtttccctc atacaaggga cctttccaat
     8941 agttgttacc aggaagcttt cgtaatacca ggaagttcac cgcggtgtgc catctcgcgg
     9001 aagcagactc ggcagatgcc gaatttctga tagaccgaat gtggtcggcc acaacgttgg
     9061 caacgtgtgt atccacgaac cttaaattta ggtttgcggc ttgccttaac cttcagtgat
     9121 gtctttgcca ttatttattt ctccccttga tccttaaacg gaaaaccaag cgccttaagt
     9181 agcgcacggc cttcgttatc gttcttggct gaagttacga tggtgatatc cattccacgg
     9241 actcgatcta gcttgtcttg ctcaatttca agaaagacga cttgctcagt tagaccgaag
     9301 gtgtaatttc ctttgccatc aaattgcttt ggagaaaggc cgcggaaatc gcggattctt
     9361 ggcagagaaa ctgttaatag ccggtccata aactcccaca tccggtcccc tcttagggtg
     9421 acatgggcgc caattggctg gccttcacgg agtttgaagt tagcaattga ttttctcgcc
     9481 ttagtaacta atggacgctg tccagtaatt gcggcaaggt ctctaattgc gccttcaatt
     9541 aactttgaat ccttagctgc ttcgccaacg cccatattga ccacaatctt ggaaagggtt
     9601 ggaatctgca ttctatttga gaagttaaat tcgctctgca gggcaggagc aacttcagat
     9661 ttatagatct cttttaaacg aggtgcaata acagtactca tttatagatc ccctcctgtt
     9721 cttctagaga tacgaatgtt cttgccttct tcatttttgc gaactgcaat ccgggttgcc
     9781 ttattatcat catctagcaa catcacattg gagtaagcaa ttggggcttc aatagtgaga
     9841 attccgccaa ctttggcacc gcgctgtgaa gtatcttctt ttgtatgctt cttgacgcgg
     9901 ttaacacctt caacagttac gcgggcagta acggtattaa cggctaatac tttgccagtt
     9961 acacctttat cttttcctgc gatgactaga actgtgtcac ctttctttat tctcattaca
    10021 gcacctccgg ggcaagtgag atgatcttca taaagcgctt atcacgaagc tctctagcaa
    10081 caggtccgaa aatacgggtt cctcttggtt cgttatcttc cttgataatt accgcagcat
    10141 tctcatcaaa tttaatgtag gaaccatcag ctctacggtt ctctttaaca gttcgaacga
    10201 tgacagcctt aacgacttca cctttcttaa cctgtccgcc aggaattgca tctttaacgg
    10261 aacagacaat gacatcaccg attgaggcat agcgacgttg ggttccacct agtacgcgaa
    10321 tacagagaag ctctctagct cctgtgttat cggcgactcg tagtcgtgac tctgcttgga
    10381 tcatggttac ttagccttct cgataatctc cactacacgc caacgcttag ttgccgaaag
    10441 tggacgggtt tccataatgc gcacgcgatc tccaacaccg gcggtgttat cttcatcgtg
    10501 agccttaaac ttcttattct tggtaataac tttggagtag aggccatgag cagcgcggtc
    10561 ttcaaccttt accaccacag tcttatccat cttgtcgcta acaactacgc cttcgcgagt
    10621 cttgcgaaac tttctatcct cactcatgcc gctcctccaa ttcctagttc gcgttcacga
    10681 ataacggtat agatcttggc aatctcttta cggaccgcag ttaggcgtcc atggctctca
    10741 agttgaccgg ttgcagcttg gaaacgaagg ttgaataact cttcctttga atcctttaac
    10801 ttctctgcaa gatcttcagt gcttagagct ctaagttctt cagtagtgat catctttgcc
    10861 atggttatac ctcctcgcga attactacgc gagatttgac tgggagttta tgagctgcgc
    10921 gagagagcgc ttgatttgca acatctagcg ttacgccaga tatttcaaat aaaactcttc
    10981 ctggtttcac atttgcgatc cagaattctg gagatccctt accagaaccc atacgggttt
    11041 cagcaggctt cttagttagt gggcgatctg gatagatatt gatccaaacc tttccaccgc
    11101 gcttgatatg acgagtcatt gcgatacgag ccgcttcaat ctgacggtta gttacataac
    11161 caccctcagt tgcttgcaga ccaaaatcac cgaatgcaac cgctgttccg cccttagcat
    11221 ttccagaacg ctttggacga tgttgcttgc gatgcttaac gcgacgtgga attaacatta
    11281 tgcatcactt ccttcgcttt taacttcagg gcttacttcg ctttcaacag cagctgcagc
    11341 ttcatttgct cgttctgcaa cagatgtctt tgtcacttca ccctttggcg cgcgaggtgc
    11401 acgctttggt gcgcgctcag ccttagctgc ttgaacttgc gcaagagcag ctgcttcgcg
    11461 ctcagttctt gaagcgataa cttcgccctt ataaatccag accttcacac ctaagcgacc
    11521 aaatgtggtg tgggcttcag caaagccgta atcaatatcg gctcttaggg tatgaagtgg
    11581 aactcttcct tcgcgataga actcagagcg tgacatttct gccccaccaa gacgtccacc
    11641 gcattggata cgaacaccct ttgctcctgc tttaaatgca gtctgcattg ctttacgcat
    11701 tgcgcggcgg aaagagacgc gagctgctaa ttgctctgca actccgcgcg ctactagctg
    11761 ggcatcaatt tcaggattct taacttccaa gatatttaac tgaacttgct tgccagttaa
    11821 tttctcaaga tcaagacgga gacgatctgc ctcagttccc ctacgtccaa tcacaattcc
    11881 aggacgagcg gtgtgaagat cgatacggac gcgctcacga gtacgctcga tttcaattcg
    11941 agatactcca gccctctcca tgttcttctc catcatgcga cggatcatca catcttcttt
    12001 tacataatct ttataaagct tctcggcata ccagcgagac ttgtactcag tggtgattcc
    12061 taggcggaag ccatgagggt tgattttttg acccatttac ttcgcctcct gctctgtagt
    12121 tttcttagct gctgcctttt tggcgctagc ctttttaacc ggagctttct ttgctgctgc
    12181 aggtttctca tctgccacaa cttcactagc tgctgcaaga gctgctgctg ctaattttct
    12241 tgcctgctct tttgttggct ttcgataatt cttatcatca gatagaacta cagaaatctg
    12301 tgaagagcgc ttcaagatct gatagccacg gccctgggcg cgcgggcgat agcgcttcat
    12361 tgtgatgccc tcatcaacta gagcctcggt tacccagagc tctgaggcat ctctaattgc
    12421 aggatttttc tgcttagcat ttgccacagc agagttaagt agtgaataaa gatcttcact
    12481 tactgcaatc tgtggtgaga acttagcaat tcttagcgcc tcatcagctc tctggccgcg
    12541 gatcatattt actacgcgac gagctttctg aggagttaca cgaattgagc taagtgatgc
    12601 tttagctact aaaggagtat tactcggcac gggtcaccgt ccgatcttct tgtctgacgt
    12661 gtcctctaaa tgttcttgtt ggtgcaaact caccgagctt gtgaccaatc attgcctctg
    12721 tgacatagac cggaacatgc ttgcgtccat catggactgc aatggtgtga ccaatcattg
    12781 atggcgtgat cattgagcga cgtgaccaag tcttaataac tgtcttggtg ttcttcgcat
    12841 tctgttcatc caccttcttt tccaggtgta gatccacaaa gggacccttc ttcaaactac
    12901 gtggcatttg gtttcagggc tccttatcgg ctcttattag ctggacgacg acgaatgatc
    12961 aattgatcac tagatttttt cttacgagta cgagcctcag gtttacccca aggtgaaaca
    13021 ggatggcgac ctccagaggt ctttccttct ccaccaccat gagggtgatc aactgggttc
    13081 ataacaacac cacgaaccgt tggacgacgg cctttccagc gcatacgtcc tgcttttcca
    13141 tggttaatat ttgattgctc agcattacca acaacaccga ttgttgcgcg gcatctgaca
    13201 tcaacatttc taatttcgcc agatggcata cggagctggg catatggacc atcttttgca
    13261 actaactgca cagatgctcc agcagatctt gcaattcttg ctcccccacc tgggcgagtt
    13321 tcaattgcat gaatcattgt tccaactgga atatttctta gcggcaagtt attgccaggc
    13381 ttaatatctg cagttgggcc attttcaatc tttgttccct gctcaacgcc aagaggagcg
    13441 atgatgtagc gcttctcgcc atcagcaaag tgcacaagtg caatgcgggc agagcgattt
    13501 ggatcgtatt caatttgtgc aattactgca ggaacgccat ctttatcatt acgcaggaaa
    13561 tcaattacgc gataggcgcg cttatgtccg ccgccttgat ggcgagttgt gatcttgcct
    13621 gaggcgttac gaccaccgcg agagttaatc ggacgaataa gtgacttctc tggagtagat
    13681 cgggtgatct cagcaaagtc ggaaacgctc tggccacgag agctcggcgt taccggtctt
    13741 aacttacgaa gtgccatgtc atatcccttt tcttattaat cccatcgcct attactagga
    13801 gacagggcct ccaaagatgt cgatgcgatc tccagctgcg atttgcacaa tcgcgcgctt
    13861 agtatcgctt cgctttccaa ctccataacg agtgatcttg ctcttgccct cacggttcat
    13921 tgtgttgacc ttgagaacgc gaactttgaa aatcttctca actgcaatct tgatctcagt
    13981 cttattggca tcaggtgcaa cgatgaaggt gtacttattc tcatcgagca tgccgtatga
    14041 cttctctgaa accactggag caattagaac ttcgcgatga tctctcatgc caaggcctcc
    14101 tctaactctg attcaaaagc tctggctgta acgctcttgc ctttgctagg gcctgcaaca
    14161 aagtcagtga tagctttttt ggaaaatacc aagtcatcag caactacaac atcgtaggca
    14221 tttaactgat cctgacggat tagatgtaaa tcttcttcgt tgcgaagtga gcgccatgaa
    14281 gcctcttcgc catgagctag aacaacaagt actcggcgac gatctgagaa ttggcgaact
    14341 gcagcgatag cagctttgct atttacgctt tcaacaattg ttgaaacgat atgaattcgc
    14401 tcatttcttg ctcgatctga gagaacgcca gaaagtgctg ctttaatcat tttcttagga
    14461 gtgcgctcat cgtatttacg aggaactggt ccttgagcaa caccaccatg gcgctgattt
    14521 ggagaacgag ttgaaccctg acgagctcta ccagttccct tctgcttgaa aggcttctta
    14581 ccgccaccgc taacctcacc gcggtttttt gccttagcag ttccagctct tgctgcattt
    14641 agctgagcag ttactacttg atgaattagt ggcacattta cttgaacatc aaaaatttct
    14701 gcaggaagat caaaggttcc agcctttgca ccctttgcat ctttaatttc aatagttaac
    14761 gacatcttta tgcacctgcc ttattgataa cttcactaag agccttcttt gaagctgagt
    14821 gaataaagac ctgcgctcca gttcttccag gaattgcgcc tttaataaga attagtgatt
    14881 tctcaacatc aactgcttgc acagttaagt tttgaaccgt aacagtctca acgcccattc
    14941 gacccatcat gcgctttcct ttaaatacgc gtccaggtgt ggtgcggtta ccgatcgatc
    15001 ctgaggtgcg gtgctttctc tcaacaccat ggccatcgcc aaaacctctg aaattgtggc
    15061 gcttcataac accagctgat cccttaccca gagatgttcc ttgagcatca acgatttcgc
    15121 cagctgtgaa aatatctgct ttaacttctt gtcccacagt gtaggaagaa gcgctagctg
    15181 ttcttagctc tgcgacaaac tttcttggag taactccagc tttagcaaat tgaccagatt
    15241 ctggcttaga aacttttctt ggatcaattg cgccaaatgc caattgcaca gcttcatatc
    15301 catctttttc cagagtgcgg atctgagtca caacacatgg acctgcctga acaacagtta
    15361 ctggaatagc ttggctattt tcatcaaaga cttgagtcat gccgagcttg acgccaagga
    15421 ttcccttgat ggcgctgccc tttgattgag tagttgtcat agttgtcgag gtccccttag
    15481 agcttaatct caatatcgac gccagcagga agatcaagac gcatcaagga atcaactgtc
    15541 ttaggagttg gatcgatgat gtcgattagg cgcttatggg tgcgcatctc aaaatgttcg
    15601 cggctatctt tgtatttatg tggcgagcga atcacgcaat aggtgttctt ctcagtaggc
    15661 agcggaactg ggcctgcgac cgttgcccct gtgcgctcaa ctgtttcaac gattttcttc
    15721 gctgaagagt caatcacctc atggtcatag gccttgagcc tgatgcggat cttctgtccc
    15781 gccattttca ttccctcgct tctacttaag tgcttcttgt tttaaatggg gcgcttgtcg
    15841 cgccccattt aattacttct ttttttctct acttaacgat ctttgttaca cgacctgcgc
    15901 caacagtgcg tccaccttcg cggatagcaa aacgcagacc ctcttccatc gcaactggct
    15961 ggattagagt cacagtcatt gcagtgttgt caccaggcat aaccatttcg gttcctgatg
    16021 gaagtgttac tacgccagtt acatccgtag tgcggaagta gaactgagga cggtagttgt
    16081 taaagaatgg tgtgtgacgt ccgccttcat cctttgaaag gatgtaagaa gatgcctcaa
    16141 agtcagtgtg tggtgtaacg ctgccaggct tgcaaacaac ctgtccacgc tcaacatctt
    16201 cacgctttag accacgaaga agtagaccaa cgttttcgcc agcctgacct tcatcaagaa
    16261 gcttgcggaa catttcaact ccggtgattg tggtcttctg tgaatctgga cggataccaa
    16321 caatttcaac ttcttcgttg accttgacga taccgcgctc aatacgacca gttacaacag
    16381 ttcctctacc agtgattgtg aatacatctt ctactggcat caagaatggc ttatcaactt
    16441 cgcgtactgg ctgaggaatg aatgtatcaa cagcattcat aagttccatg atcttctcgc
    16501 cccatgcagc gtctccttcg agagccttga gagctgagat gcgaacaatt ggagtgtctt
    16561 ttcctgggaa ttcatattta gttagtagat cgcggacttc ttcctcaact agaccaagga
    16621 tgtcctcgtc atcaaccatg tcagacttat taagagcgac aacaattgat ggaacgccaa
    16681 cttggcgagc aaggagaacg tgctccttgg tctgtggcat tggtccatct gttgcagcaa
    16741 ctacgagaat tgctccatcc atctgagctg caccagtgat catattcttg atgtagtcag
    16801 catggcctgg acagtcaacg tgtgcgtagt gacgcttctc tgtctggtat tcgatatgtg
    16861 caatcgaaat ggtaatacct cttgcgcgct cctctggagc cttatcgatc tgatcgaatg
    16921 cgcttacctc attgaggttt gggtatttgt cgtgcagcac cttggaaatc gcagcagtaa
    16981 gagtggtctt accgtggtcg atgtgaccaa
//
LOCUS       DAOROD010000022        16233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_141_length_16233_cov_27.378724, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000022 DAOROD010000000
VERSION     DAOROD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 16233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16233
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_16233_cov_27.378724"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLW53_04985"
     CDS             complement(<1..331)
                     /locus_tag="PLW53_04985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRD36808.1"
                     /translation="MEQEDHQRDLTRTINMGSLRPVSDQLSLLLDKTSQEERDVINAL
                     GEGSAMLISLSGPGKGARFLINSDRTVIGRAVDCDIFLDDVTVSRKHAEVIRNGKSYS
                     FKDSGSLN"
     gene            complement(422..805)
                     /gene="gcvH"
                     /locus_tag="PLW53_04990"
     CDS             complement(422..805)
                     /gene="gcvH"
                     /locus_tag="PLW53_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRD36809.1"
                     /translation="MANVPDQLKYTKEHEWVSEVSTNRYRVGITDYAQVALGDIVYIQ
                     LPKLGDQIGAGKVCGEVESTKSVSEIYAPLSGKVVLVNDALDSAPETLNKEPYGAGWI
                     FEVEISAPSELESLLSAGDYASLTA"
     gene            complement(808..1356)
                     /locus_tag="PLW53_04995"
     CDS             complement(808..1356)
                     /locus_tag="PLW53_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD36810.1"
                     /translation="MLTIPNLLTMARALGIPVFLYLVLVKEEMGLAIFTLVVAGATDY
                     FDGKLARAWNQESRLGELMDPAVDRLYIISVLIAMFATQVVPLWVLALIAGRDIFLGL
                     LLVVMKTKGIPPFKVTYLGKAATFNLLYALPLLLLTDSTSGSISDAAYIFGWGFAGWG
                     IGLYLLTGLSYARSGIKSLKRG"
     gene            complement(1358..1431)
                     /locus_tag="PLW53_05000"
     tRNA            complement(1358..1431)
                     /locus_tag="PLW53_05000"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1395..1397),aa:Pro,seq:ggg)
     gene            complement(1468..3438)
                     /gene="der"
                     /locus_tag="PLW53_05005"
     CDS             complement(1468..3438)
                     /gene="der"
                     /locus_tag="PLW53_05005"
                     /inference="COORDINATES: protein motif:HMM:NF002828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRD36811.1"
                     /translation="MTVVVAMDGPSGSGKSSTAKAIAKRAGWLYLDTGALYRAATWLA
                     IEKAIDSADELLVALKTSPILFKTDPSNPITLCGGVDVSEAIRSAEVTSAVSRVSSWP
                     ELRKELVALQRNIIESAKNGIVVEGRDIGTVVAPGANLKIWLQADINARAIRRDLEDK
                     SRGLETSSPDSVAASLSSRDQVDSTRAVSPLRKAEDAIIVDSTNLTLDETVAYIWNIL
                     KSRSLIGLPRVVIVGRPNVGKSTLVNRIIGSREAIIEDTPGVTRDRVSYEAEWNGKTF
                     MVIDTGGWEPTSEGIALKISDAAESAISDADLVLFVVDAQVGQQSDDDALISVLRKSK
                     KPVMLIANKVDSEKDELDAHSLWNLGLGEPHFISAAHGRGSGDLLDLIVSQLPEVGAS
                     RIDDGYRRIAIVGRPNVGKSSLLNSFAGSTRSLVDDAEGTTRDPVDELIDLGDRTWRF
                     IDTAGIRRRAHQASGSEYYAALRTERAIENAEVVIMIIDASTPITEQDLRIITMVEDS
                     GKALVIGLNKWDLVDEDRRVVLDKETERNFDQVEWAERVNISAKTGWHKDKLLPAIQR
                     ALESWEARVPTGRLNSFLGQLVGSTPPPLRGGKQPKILFATQAGIAPPTFVIFATEFL
                     EASYRRFIERRLREEFGFSGSPIRVSVRIRER"
     gene            complement(3435..4523)
                     /locus_tag="PLW53_05010"
     CDS             complement(3435..4523)
                     /locus_tag="PLW53_05010"
                     /inference="COORDINATES: protein motif:HMM:NF005112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRD36812.1"
                     /translation="MNAQASPQIQSVKIVGAGLIGTSIALALAHQGFLVSIDDADQKA
                     LALANDLLAPYLKAKDEEKREFDLVIIATPPASVVSALKAEFARNSKSIFIDVSSVKT
                     NLIQEIDALPALGERFVGTHPMAGREISGASSAQADLFNGRAWIITPTTKTSTRAIAM
                     AKELIQVLGASSYEMSAREHDHLMARISHLPQIASTLIASEIQELGAGLELSGQGLRD
                     MTRIAGSDGALWSEILLENKNEVLSTLDKFISKALALKGAISESKQEEIKTLFLKGNS
                     GRAKVSGKHGAKPRNYLHLLVVIKDEPGALSQLFTECAQIKANIEDLTIEHSPGQESG
                     LITLAFSPEDGERVEAHLKKQDWKVHRR"
     gene            complement(4523..4885)
                     /gene="aroH"
                     /locus_tag="PLW53_05015"
     CDS             complement(4523..4885)
                     /gene="aroH"
                     /locus_tag="PLW53_05015"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRD36813.1"
                     /translation="MFVRAIRGAIQIDRDDPVLMQEAVVELVSKVLEANQLTSTDLIS
                     LLFTCTPDLVSDFPAASARAMGLEKVPLICAVEMAVPTSLPRTIRLMLHCHTKLNQDQ
                     ISHIYLRGAVTLRKDIAQ"
     gene            complement(5168..5863)
                     /locus_tag="PLW53_05020"
     CDS             complement(5168..5863)
                     /locus_tag="PLW53_05020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRD36814.1"
                     /translation="MADLVRLQKYMADCGVASRRACEELIDQGRVKVDGKVIRTQGLK
                     IDPIKTKVEVDNETINAVKTKTYLAFHKPLGVLSTMSDPEGRPCLSDYFLDWQERLFH
                     VGRLDKESEGLIIVTNDGQWAHEATHPSFEVKKTYLVATEETLPRAAFDLLSRGVELD
                     DGLARPLAVNRFGGFIELVIHEGRNHVVRRIFDEIGFPVTRLIRTAFGSIKLGELPSG
                     KWRALNEVEITNL"
     gene            complement(5879..6436)
                     /gene="scpB"
                     /locus_tag="PLW53_05025"
     CDS             complement(5879..6436)
                     /gene="scpB"
                     /locus_tag="PLW53_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRD36815.1"
                     /translation="MSNRELERSIEAILMVVEEPVSEVVLAQIIEAPTEQILEALNAL
                     AASYEEQARGFTLKQISGGWRFYSHPDLAPLVEKFVLDGQQAKLTQAALETLAVIAYR
                     QPISRARVSAIRGVNVEAVMKTLVTRGLVEECGIEHETGAILYRTTHYFLERIGLNRV
                     EDLPALAPFLPNIDGLDEVIASLTE"
     gene            complement(6429..7337)
                     /locus_tag="PLW53_05030"
     CDS             complement(6429..7337)
                     /locus_tag="PLW53_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRD36816.1"
                     /translation="MNEVSAQPSSNTLASPAQSISEGRVAGFSVHLENFDGPFDLLLQ
                     LISRHRMDITDVAIATVTDEFISYIRELEKTEAGWKLDHASEFVVVAATLLDLKAARL
                     LPSGQIDDEEDLALLEARDLLFARLLQYRAFKEIASILSERIIEQEKTFARSVSLDPV
                     FAALLPEVLIGVTPERFAAIANRVLTPKTAPTLSVEHLHLPLVSVAAEALGVVSALRR
                     HKTMSFRALVSDAANTLVVVARFLALLELFKEGSVRFEQVVALGELQITWVGSVEGEI
                     RVSDEFDRPALVESGEDGVEEGGENV"
     gene            7400..8317
                     /locus_tag="PLW53_05035"
     CDS             7400..8317
                     /locus_tag="PLW53_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD36817.1"
                     /translation="MAKSASEISRLNAKSTFSKADSEIVTSSSVLGKPAKDFPYPAPL
                     TEHGDAKIISVVNQKGGVGKTTTTINLGAAFAELGRRVLLIDFDPQHSLSIGLNVSTR
                     DIEGSVYNLLMDESTQVSDFLLKTSVPGMDMIPAHEDLSGAEGGLVNVIAREKILKRV
                     LSTVTDFYDVILIDCQPSLGLLTVNALTASHWVLVPLECEYFALRGVALLDDIIKKVK
                     ANTNPDIELLGILPTMFDRKTVHSREVLERISEAFPTQVFDTTIARTVRFPETTVAGE
                     PITSYASSSVGAASYRRLAREIIANGGVR"
     gene            8314..8730
                     /locus_tag="PLW53_05040"
     CDS             8314..8730
                     /locus_tag="PLW53_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36818.1"
                     /translation="MTRRISLPGADELFRKTTLSAVPDQSVEEVEVAHTNRPTTATVA
                     KAAPKSTGVRQTPRRRATGLDKGPSGREKHDEKITVYLSPEELFDLEQARLHLRGDLG
                     LAVDRGRIVRESLAIVIADLEAKGDQSIIARRLRGR"
     gene            complement(8733..9641)
                     /gene="xerD"
                     /locus_tag="PLW53_05045"
     CDS             complement(8733..9641)
                     /gene="xerD"
                     /locus_tag="PLW53_05045"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRD36819.1"
                     /translation="MDDRSSIEIFLNHCAIERGLAKNTLSSYRRDLEKFLSYLNQNQK
                     TLSSITPSDVAAFLHTLRQANLNESSIARNAVALRSLFAFLAKESGVESIAKEILVPR
                     SAKRLPKALSIDQIATLINSCADDLSGIRDRAILELLYATGARVSEIIALNRSDISQN
                     NQEGVEAITIRVRGKGGKERLVPVGSYARLALDQYLTRSRPVFAKNLKEEALFLNEKR
                     GTRLSRQSAWQIVIDAAKRSGLGEAISPHGFRHSFATHLLDGGADIRVVQELLGHASV
                     TTTQIYTLVTIDKLRESYASAHPRSK"
     gene            complement(9634..11265)
                     /locus_tag="PLW53_05050"
     CDS             complement(9634..11265)
                     /locus_tag="PLW53_05050"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRD36820.1"
                     /translation="MTAPHVTKHLFVTGGVASSLGKGLTASSLGRLLVARGLRVTMQK
                     LDPYINVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDTNLHGSANVTTGQIYQR
                     VIARERHGEYLGETVQVIPHITNEIKERIRAMAAPDIDLVITEVGGTVGDIESLPFLE
                     AARQIRQEVGRDNVFYLHVSLVPYIGPSGELKTKPTQHSVQALRSIGISPDGIVLRSD
                     REIPQGVKKKISLMCDVDLEAVVAAVDAPSIYDIPKVLHSEGLDSYIVRRLDLKAHEV
                     KWGQWDSLLERVHNPAHSVKVALVGKYVDLPDAYLSVTEALRAGGFANNARVEIKWVA
                     SDECATEAGAKANLSEVDAICVPGGFGVRGIEGKLGALKYARENKIPALGLCLGLQCM
                     VIEIARNILGLKRANSAEFDPDTDAPVIATMASQVKIVNGEADMGGTMRLGLYKAILS
                     KGSIVESCYQAPEISERHRHRYEVNNSYRKELSEAGLVFSGTSPDENLVEFVELPREV
                     HPFYVGTQAHPEFRSRPTRAHPLFAGLIKAALERG"
     gene            complement(11280..13031)
                     /gene="recN"
                     /locus_tag="PLW53_05055"
     CDS             complement(11280..13031)
                     /gene="recN"
                     /locus_tag="PLW53_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRD36821.1"
                     /translation="MSKKGERKGSLLEEINIRSLGLIDSATIEFKEGFNVITGETGAG
                     KTMLLNALSLILGQKADADYIRQGQERAVVSGRFRLPEKISEDLKVLIQEHEPEIEES
                     AILLSRQLGRDGKSKAQLSGSATTASTLISFADQLIEIHGQHANLVLTKANKQREILD
                     IFAGQGLSTALLAYQEALKNYQVLRREISELKKALGDRDSEIKSLEELVNDFAKLKPQ
                     SSELSQLEAQISKLESVEDLRIASLGAQQALSDEEAGALNSLHSAKKSLTGAKGRDPQ
                     LDQIADAISEKLFDLIDAASDLDRYLDSLSADPQALELAQSRRAALSSFAKKYGKSSD
                     KNEALAEAIDKAEGAKNRIKDLSGGDDRLAEMGRELEGMRAKLLEAAKTLSALRAQSA
                     KKLSSSVTKELVELAMAKAIFEVRVESRDGSSDDHFSLFGLDEVFMLFTAHSGGELLP
                     VSKAASGGELSRLMLALEVVLAGSYPLGTYVFDEVDAGVGGKAALEVGKRLRKLAMNS
                     QVIVVTHLPQVALWADNHILVEKDSNGLLSSTSIKSLSDQEREIEIARMLSGIEESEH
                     AQDHARELLNLRFSDVG"
     gene            complement(13024..13893)
                     /locus_tag="PLW53_05060"
     CDS             complement(13024..13893)
                     /locus_tag="PLW53_05060"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002892.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRD36822.1"
                     /translation="MTKREVLLVLHPTRPEAKELAGSIVKTLSAEGFSFISSADTGIS
                     GVTQVASEQVRQRYGEIELVLVLGGDGTILRGAEQIHGSNIPVLGINLGHVGFLAEIG
                     KPSQTEIISAIRDKKYKTEKRMTLAYQVFRGDELVTKGWALNEVTVERSTEAMIELFV
                     QVDHRPLSRWGCDGVICATPTGSTAYAFSAGGPVVWPEVEALVLLPLAAHALFSDPMV
                     IGADSEIAIDVESDEGVLSADGLRKFALKEKDRVVLTSDKSYINLAHIDAAPFTDRLV
                     AKFKLPVDGWRGE"
     gene            complement(13894..14682)
                     /locus_tag="PLW53_05065"
     CDS             complement(13894..14682)
                     /locus_tag="PLW53_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRD36823.1"
                     /translation="MRTRLDAELVRRGLARSREDAVDLIESRSVLVRGIPATKPATQV
                     DPETSITLAGKREDYVSRGGYKLAGALDAFKEIRVSGVTALDAGASTGGFTDVLLKRG
                     AAKVIAVDVGYGQLAWELQNDPRVEIHDRTNVRTLSPEIIKESVDLVVADLSFISLAL
                     VIPALLSVSKSEADFLLMVKPQFEVGRERLTDGGVVRDPELRKAAVRDIARSAFDAGL
                     GTVGAVASCLPGPSGNVEYFLWLKSGAEMISDKELDLAIAKGPQ"
     gene            complement(14727..14897)
                     /locus_tag="PLW53_05070"
     CDS             complement(14727..14897)
                     /locus_tag="PLW53_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36824.1"
                     /translation="MSENIENLEQISAIKKRINEIAFAPLAEHGALFDQVNQDLSAAL
                     THVEGISSQSQS"
     gene            complement(14894..15217)
                     /locus_tag="PLW53_05075"
     CDS             complement(14894..15217)
                     /locus_tag="PLW53_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36825.1"
                     /translation="MANDLLSALRTYLTKLSSGEKSPQEIAAALNQWAKESGEAIKTR
                     VEEEVKTSVKKMGFAKQEDLDRLAREVELLKAKLVTTPRASKPAKKKAPAKKSAAKKS
                     ASKKK"
     gene            complement(15246..16097)
                     /locus_tag="PLW53_05080"
     CDS             complement(15246..16097)
                     /locus_tag="PLW53_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36826.1"
                     /translation="MVERPARPSYNKSSDRRPSSRKPSDRKPSDRKPSDRKPSDRRGS
                     DRSGPRPARGGVGRERNDLRDENRRVRKFAEPEIPIEVTGKELEKRDAFALGTLAAEN
                     GEAVAKHLVCINLFLESNPERAFWHGQAAVFRAGRVAIVRERAGIAALNFQKYEIAQK
                     ELRAAIRISGSQSALPYLAQAELALGNPRKTLEIAGSIDPKLLTQGERVELRIAAAGA
                     RSALGQSDAAVVTLTCPELTLSDASFAAKLQGAYIQALIAAGRAGEAEEFRVKYQKIE
                     PSEESKG"
BASE COUNT         4289 a         3975 c         3359 g         4610 t
ORIGIN      
        1 catttaaact tccgctatct ttaaagctat agctctttcc atttctaata acttcggcat
       61 gttttctaga taccgtgaca tcatctaaga aaatatcgca atcgactgct cttccaatca
      121 cagttctatc tgaattaatt aaaaagcgag ctccttttcc gggaccgctt agtgaaatta
      181 acattgcaga accttcgcca agtgcgttga ttacatctcg ctcttcttgg ctggtcttat
      241 caagcagtag cgagagctga tctgaaacag gccgcagtga gcccatattg atagttcgag
      301 ttaggtcgcg ctggtgatct tcttgctcca cagcgcctcc ttactctcaa ccaaaggcta
      361 aggctcaaga gagcctcacc gttatcttga ggctgacact aggcgcgaga taaggggtga
      421 gtcaagcggt gagagaggcg taatcccctg ctgagagcaa gctctctagc tcacttggag
      481 cagaaatctc aacttcaaag atccagcccg caccataagg ctctttattc aaagtctctg
      541 gggcgctatc taatgcgtca tttactaata caactttgcc acttaatggg gcatagattt
      601 cagagacgct cttagtagat tccacctcgc cgcagacttt gccagcgcct atctgatcgc
      661 caagttttgg caattgaata tagacaatgt cgccaagtgc tacttgagca taatcagtaa
      721 ttcctacccg ataacgatta gttgagactt cactaaccca ttcatgctct ttggtgtatt
      781 tcaactgatc tggaacgttt gccacaacta tcccctcttt aagctcttaa tacctgagcg
      841 agcgtaactt aaaccagtta ataggtataa cccaattccc caccctgcaa agccccatcc
      901 aaaaatgtag gcggcatcag aaattgaccc tgaagtggaa tcagtaagaa gaagtaaagg
      961 aagggcatag agcaggttaa aggttgctgc ctttccaagg taggtaactt taaacggcgg
     1021 aattcccttt gttttcatca ctacaagaag aagtcctaga aagatatctc tgccggcaat
     1081 caaagccaaa acccagagcg gaaccacctg ggttgcaaac atcgctatca aaaccgaaat
     1141 tatgtagagg cgatcaaccg ctggatccat tagctcgcct aatcgagact cttgattcca
     1201 tgcccttgca agttttccat caaaatagtc agtagctcca gcaacaacta gggtaaaaat
     1261 cgcaaggccc atctcttctt ttactaaaac aagatagaga aataccggaa tacccaaggc
     1321 gcgggccatc gttaataagt ttggaatagt tagcacgtcg ggacgagagg atttgaacct
     1381 ccgaccacat gacccccagc catgtgcgct accaagctgc gctacgtccc gcaaagactt
     1441 tgattagtgc agatactact taatcactca acgctctcgt atacgaaccg agacccgtat
     1501 gggcgacccg gaaaacccaa attcttcacg gaggcgtctt tcgataaagc gacggtatga
     1561 ggcctccaga aattcggttg cgaaaatgac gaaggtaggc ggcgcaattc cagcctgagt
     1621 agcaaagaga attttaggtt gtttcccgcc acgtagaggt ggtggcgtgc ttcctactaa
     1681 ttgcccaaga aaagaattaa gccttccggt tggaactctt gcttcccaac tttcaagtgc
     1741 tctttgaata gcaggcagta atttgtcttt atgccagcca gtcttggctg aaatattgac
     1801 ccgctcagcc cactccacct gatcaaagtt tcgctcagtt tctttatcta gaactactcg
     1861 ccgatcttca tccactagat cccatttatt taggccaatg actagagctt ttccagaatc
     1921 ttcaaccata gtaatgattc ttaaatcctg ctcagtgata ggcgttgagg catcgataat
     1981 catgattacc acctcagcat tttcaatagc cctctcagtt cgaagggctg cgtaatactc
     2041 agagcccgag gcttgatgag cccttcttct aatgcccgca gtatcaataa agcgccaggt
     2101 cctatcgcca agatcaatta actcatctac tggatccctt gttgttcctt cagcatcatc
     2161 aactaaagat cgagtagagc cagcaaaaga atttagaagg ctgctctttc caacattagg
     2221 tcttccaaca attgcaatcc ttcgatatcc atcatcgatt cggcttgctc caacttctgg
     2281 cagctgtgaa actattaaat caagtagatc cccgcttcct ctgccatgtg ctgcagagat
     2341 gaagtgaggc tcacctaaac caagattcca tagcgaatgg gcatcaagtt catctttctc
     2401 agaatcaact ttattggcaa ttaacatcac aggttttttt gatttgcgaa gcacagagat
     2461 gagggcatca tcatctgatt gctgtccaac ttgggcatca acgacaaaga gaactagatc
     2521 agcatctgag atagctgact ctgcagcatc tgagatttta agagcaatac cttcagaggt
     2581 tggctcccaa cctccggtat cgatgaccat aaaggttttg ccattccact ctgcctcata
     2641 gctaactcga tctcttgtta cacctggggt atcttcaata attgcctcac gagaacctat
     2701 gatgcgatta accaaagttg atttaccaac atttggccgg ccaactatca caactcttgg
     2761 aaggccaata agacttctgc ttttcagaat attccagata taggcaacag tttcatcaag
     2821 agttaaattc gttgaatcaa cgatgatggc atcttctgcc tttcttagtg gcgatactgc
     2881 gcgcgtgcta tcaacctgat ctctacttga tagagatgct gcaacgctat ctggcgagct
     2941 tgtctctaaa cctcttgatt tatcctctaa atcgcgcctt attgcccttg catttatatc
     3001 tgcctggagc cagatcttta agtttgcacc aggggcaaca acagttccaa tatccctgcc
     3061 ttcaacaaca attccattct tagcgctctc aataatattt ctctgaaggg ctaccaattc
     3121 ttttcttaac tccggccaac tactcactct agaaacagcc gaggtaactt cagcacttct
     3181 tatcgcctca gatacatcca ctcccccgca tagcgtgatg ggattgctcg gatcagtttt
     3241 aaagagaatt ggagaagtct taagcgccac caataattca tctgcgctat ctatcgcttt
     3301 ctcgattgcg agccaggtag cagcgcgata aagcgcgcca gtatctagat ataaccaacc
     3361 agctcgcttt gcaatagctt ttgcagtgga tgattttcca gatccactag gaccatccat
     3421 cgcaaccact actgtcatcg gcgatgaacc ttccaatctt gcttctttaa gtgagcttca
     3481 acccgctcgc catcttcagg agagaaggct agtgtgatta aaccactttc ttgaccgggt
     3541 gaatgctcaa ttgttaaatc ttcaatattg gctttgattt gagcgcattc agtaaataat
     3601 tgacttagag ctcctggttc atctttgatg actactaata ggtggagata atttcttggt
     3661 ttagcgccat gtttgccaga aacttttgct cgtcctgaat ttcccttaag aaagagagtt
     3721 ttaatctctt cttgtttcga ttcagaaatt gcgcctttga gtgccagcgc tttggaaatg
     3781 aatttatcga gagtgcttag aacttcattt ttattttcta aaagtatctc gctccaaagc
     3841 gccccatcac ttccagcaat tctggtcatg tctctaaggc cctgccctga gagctcaaga
     3901 cctgcgccta gctcttgaat ttcactggct attagcgtgg atgcgatttg aggcaggtgt
     3961 gagattctgg ccattaaatg atcatgctct ctagcgctca tttcataact acttgcccca
     4021 agaacctgaa ttagttcttt tgccattgca atggcgcggg tagaagtctt ggtagttgga
     4081 gtaataatcc acgctctgcc attgaaaaga tctgcttgag cggaagatgc tcctgaaatt
     4141 tcgcgaccag ccattggatg agttccgaca aatcgctctc caagcgccgg taatgcatct
     4201 atctcttgta taaggttagt ttttacactg cttacatcta taaatattga tttagagttt
     4261 ctagcaaatt cggccttaag ggcagataca acagatgctg gcggagttgc gatgataact
     4321 agatcaaatt ctcgtttctc ttcgtccttg gcctttaaat acggcgccag gagatcgttc
     4381 gcaagagcta aagctttctg atcggcatcg tctatggaga ctaagaaccc ttgatgcgct
     4441 agagctagcg cgattgaggt accaattaag ccagcaccta cgatttttac tgactgaatc
     4501 tgaggagaag cttgcgcatt cattactgag ctatatcttt gcgaagagtg accgcgccgc
     4561 gaaggtagat atgtgaaatt tgatcctggt tcagctttgt gtggcagtgg agcatcaaac
     4621 gaatagttct tggaaggctt gttggcacgg ccatttcgac cgcgcagatt aaaggaactt
     4681 tctcaaggcc catggctctg gctgaagcag ccggaaagtc gctaaccaga tctggggtgc
     4741 aggtaaaaag cagagagatc agatcggtgc tagtgagctg attggcctca agaacttttg
     4801 aaactagttc aacgacagcc tcttgcatca agaccggatc atcacggtct atctgaatag
     4861 ctcctcgtat tgctcgcacg aacactttca gattttaccc gtcttgccct ctctcccgtg
     4921 ggaagccgtt atagtgtttt atcgacttat ggttatctca gttttaggcg tctcaatcga
     4981 gattatcgac aatttcgcgc aggtgcagcg ttcggggcta aaaaccatat atcgattttt
     5041 cgggtggtta gaacgctcgg aaatatcgac atatctatgt tttggactcc tgcaaaaaac
     5101 gttatatcga taaagcagta tttttgatta tatataaatg gttaaaacta taaaacaatt
     5161 aaacggttta tagatttgta atttcaacct catttagcgc tcgccatttg ccactaggta
     5221 gctcccccag ctttatcgag ccaaaagcag ttctgatcaa gcgagtaact ggaaatccaa
     5281 tttcatcaaa gattcttcta accacatgat ttcttccctc atggatgacg agttcgataa
     5341 aacctccgaa acggttgact gccaaagggc gagctaaacc atcatccaat tcaacccccc
     5401 ttgagagaag atcgaatgct gcccttggca gagtttcctc tgtggcaacg agataagttt
     5461 tcttaacctc aaaagatggg tgggtggctt catgggccca ttggccgtca ttggtgacaa
     5521 tgattaagcc ttcactctct ttatctaagc ggccgacatg aaataggcgt tcctgccaat
     5581 caagaaaata atcacttaga caggggcgac cttctgggtc tgacatggtg gacaaaacac
     5641 ctaaaggctt atgaaacgcg agataagttt tggtctttac tgcattaatt gtttcattat
     5701 caacttcaac tttagtcttt atcgggtcta ttttaagtcc ctgagtgcgg attaccttgc
     5761 catccacctt cacacggccc tggtcgatga gttcttcaca ggctctgcga ctagctacgc
     5821 cacaatcggc catatacttt tgaagtctta caagatcggc catagcggcc catcctactc
     5881 actcagtaag agaagcaatt acctcatcta ggccatcgat attaggtaag aagggcgcta
     5941 aagccggaag atcttcaacg cgattgagtc cgattctttc caagaagtaa tgcgtagttc
     6001 gatagagaat cgctccggtt tcatgctcaa ttccgcactc ctccactagc cctctagtaa
     6061 caagagtttt cataaccgct tccacattaa ccccgcggat tgctgagact cgagcccttg
     6121 aaatcggttg gcgatatgca atcacagcaa gtgtttccag agctgcttgc gtgagttttg
     6181 cttgctggcc atccaaaacg aatttttcaa ctaatggcgc caagtcagga tggctataaa
     6241 agcgccatcc accagagatc tgcttgagag tgaagcccct tgcctgttcc tcataggaag
     6301 ctgcaagggc attgagtgct tcaagtattt gctcggttgg ggcttcgatt atctgggcga
     6361 gaactacttc tgagacgggt tcttcgacaa ccatcaagat tgcctcaatt gagcgttcca
     6421 gttcgcgatt agacattttc gcccccttct tctaccccgt cttcgcctga ttcaactaga
     6481 gctggacgat caaattcatc actgactcta atttcacctt caacgcttcc aacccaagta
     6541 atctgcagct ctcctagagc aactacctgc tcaaatctga ctgaaccctc tttgaagagt
     6601 tcaaggagag ctaggaatct agcaactaca actaaggtat ttgctgcatc acttactaag
     6661 gccctaaaac tcattgtttt atgccttctc aaagctgata caaccccgag ggcctcagcg
     6721 gccacactaa ctagcggcag atggagatgc tccaccgaga gggttggcgc ggttttgggg
     6781 gttagaacgc ggtttgcgat agctgcaaat ctctctggag taacgcctat taaaacctca
     6841 ggtagtagag ctgcaaagac tggatcgagt gagactgagc gggcgaaggt cttctcctgt
     6901 tcaatgatgc gctcagacag gatggaagca atctctttaa aggcgcgata ctgcaagagg
     6961 cgagcaaaga gcagatctcg agcttcaaga agtgcgagat cttcttcatc atcaatctga
     7021 ccagatggca ataatcgagc tgcttttagg tccaataaag tagcggcaac aactacgaat
     7081 tcgctggcat gatctaattt ccagccagct tcagtcttct caagttcgcg aatgtagcta
     7141 ataaattcat cagtaactgt ggcaattgca acatcggtga tatccatccg atgtcttgaa
     7201 atgagctgta gtaataaatc aaaaggtcca tcgaagtttt ctaagtgaac cgagaagcca
     7261 gcaactcttc cctcactaat actttgcgca ggcgaggcta aagtattact tgagggttgc
     7321 gcagaaactt cattcatagt ctggcaagtc tatgcgtgtc gcagccaata tcgcgataag
     7381 cggtggttaa agtctggcca tggcaaaaag tgcttcagag atatctcgct tgaacgctaa
     7441 atcgacattt tcaaaagctg attctgaaat tgtcacatct tcatcagtat tgggaaagcc
     7501 tgctaaggat tttccatatc cagcgccatt aacagaacat ggcgatgcca agattatttc
     7561 cgttgttaat caaaaaggtg gcgttggtaa aaccacaaca acaatcaatc tcggtgctgc
     7621 atttgcagaa cttggaagaa gagttctact aattgatttt gatccacaac actcactctc
     7681 tattggtctt aatgtttcaa ccagagatat cgagggatct gtctataacc tgcttatgga
     7741 tgagtcaacg caggtctcag atttccttct aaaaaccagt gttcctggca tggatatgat
     7801 cccagctcat gaggatttat ctggcgctga aggtggcttg gtaaatgtaa ttgctcgtga
     7861 aaagatttta aagcgagttc tctctactgt cactgacttc tacgatgtaa ttctcattga
     7921 ttgccagcca tcacttggcc ttctaactgt taatgcccta accgcatcgc actgggttct
     7981 agtgcccctg gagtgtgagt attttgcgct tagaggcgtg gctctccttg atgacatcat
     8041 caagaaagta aaggccaata ccaatccaga tatcgagctt ctcggaatct tgccaactat
     8101 gtttgataga aaaactgttc atagtcgcga ggttctagag cgaatcagtg aagccttccc
     8161 aactcaagta tttgatacca caattgctag aaccgttaga ttcccagaga caactgttgc
     8221 tggcgagcca ataacttcct atgccagctc ctcagttgga gctgcttctt atcgcagact
     8281 tgccagagaa ataattgcca atggaggggt tcgatgacaa gacgtattag tttgccggga
     8341 gcagatgagt tatttagaaa gacaactctt agcgctgttc cagatcaaag cgttgaagaa
     8401 gttgaagtag cccacaccaa ccggccaaca actgcaaccg ttgcaaaagc tgctccaaag
     8461 tcaactggcg ttagacaaac cccaagacgt cgcgcaactg gtttagataa aggaccatca
     8521 gggcgagaga aacatgatga gaaaattacc gtttatcttt ctcctgaaga actctttgat
     8581 ttagagcaag caagacttca cttaagagga gatcttggcc ttgccgttga tagaggtcga
     8641 atcgttcgtg aatctcttgc aattgttatt gcagatttag aggcaaaggg cgatcaatca
     8701 attattgcca gaagacttcg aggcagatag ctttactttg atcttgggtg agcgcttgca
     8761 tagctctcac gcaatttatc aatagttact aaggtataga tctgcgtagt tgttaccgaa
     8821 gcatgtccca atagttcttg caccacccta atatctgctc ccccatcaag aagatgggtg
     8881 gcaaaagaat gcctaaaacc atgcggagag attgcctcac ctaggcccga tctctttgct
     8941 gcgtcaataa caatctgcca ggcgctctgg cgcgagaggc gagttcctct tttctcgtta
     9001 agaaagaggg cttcttcctt tagattctta gcaaagaccg gacgagatct cgttagatac
     9061 tgatccagag ccaatctggc atagcttcca accggaacta aacgctcctt tccccctttg
     9121 ccacggactc gaatagttat ggcttcaacg ccctcttggt tattttggga aatatctgat
     9181 cgatttagag caattatctc gctaactctc gcccctgttg catagagcag ctccaatatc
     9241 gccctatctc taataccaga taaatcatct gcgcagctat ttataagtgt tgcaatctga
     9301 tcaatactta aagctttagg taatctcttt gcacttcttg ggacaagaat ctctttagca
     9361 atactttcaa cgccactttc tttggctaga aatgcaaaga ggcttctcaa agcaacagcg
     9421 tttctagcga tacttgattc gtttagattt gcttgcctta gagtgtgaag aaaggcggct
     9481 acatcacttg gagtgatact ggaaagtgtc ttctgatttt gattaagata acttaaaaac
     9541 ttctcgagat ctcttctata agaagaaaga gtgttcttag ctagccctcg ctctattgcg
     9601 cagtgattga ggaatatttc aatcgaggat ctatcatcca cgttctagcg cggccttaat
     9661 taggccagca aagagtggat gggctctagt aggccttgat ctaaattctg ggtgagcttg
     9721 agttcccaca taaaatggat gaacctctct tggaagctca acaaattcaa ctaaattctc
     9781 atctggcgat gtgccgctaa agactaaccc tgcttcactt agctctttgc gataggagtt
     9841 attgacttca tagcgatgtc tatgacgctc tgatatttca ggggcttggt aacaactctc
     9901 aacaattgat cctttggaaa gaattgcttt atagagtcca aggcgcatcg ttccacccat
     9961 atcagcctcg ccatttacga tttttacctg acttgccata gttgcaataa ctggcgcatc
    10021 agtatcaggg tcaaattcag cagagttagc tcgctttaaa ccgaggatat ttctagctat
    10081 ctcgatgacc atacattgca gacctaaaca aagtcccagc gctggaatct tattttccct
    10141 tgcatactta agagctccaa gctttccttc tatccctctt actccaaagc cgcccggaac
    10201 gcagattgca tctacctcgc ttaagtttgc ttttgctcca gcttctgttg cgcattcatc
    10261 acttgcaacc cacttaattt caaccctagc gttatttgca aagccgccag ctcttagcgc
    10321 ttcagtaact gagagataag catctggaag atcgacatat ttaccaacta aagcaacctt
    10381 tacgctatgg gcggggttat gcactcgctc aagtagtgaa tcccattgcc cccatttaac
    10441 ttcatgggct tttagatcaa gccttctgac aatataggaa tcaagtccct cgctatggag
    10501 cactttaggg atgtcataaa ttgatggagc atcaactgct gcaactaccg cctctagatc
    10561 aacatcgcac ataagagaaa tcttcttctt cacaccttgc gggatttctc gatctgagcg
    10621 aagaacaatg ccatctggag atattccaat agagcggaga gcttgtactg agtgctgtgt
    10681 tggctttgtt tttaactccc ctgatgggcc gatatatgga acaagagaaa catgaagata
    10741 aaagacgtta tctctaccaa cttcttgcct aatctgtctt gctgcctcta aaaatgggag
    10801 agattcaata tcgccaacag ttccaccaac ttcagtaatg actagatcga tatcgggagc
    10861 tgccatcgcg cgaatgcgct ctttaatctc attggtgata tgaggaataa cttgcaccgt
    10921 ctcacctaga tattcaccat ggcgctctct tgcaataact ctctgataga tctgaccagt
    10981 tgtgacattg gctgaaccat gaagattggt atctagaaat ctctcataat ggccgatatc
    11041 taaatcggtc tctgctccat catcggtaac aaatacctcg ccgtgctgga aaggattcat
    11101 cgtgcctgga tcaacattga tgtagggatc aagtttttgc attgtgactc taaggccccg
    11161 ggctacaagc aagcgaccta aactagatgc ggtaagacct ttaccaagac ttgaggcgac
    11221 tccgccggtg acaaagagat gtttggtcac atgaggggcg gtcatggaag gcaaacttat
    11281 catccaacat cgctgaagcg taaattgagc agttcccgag cgtgatcttg ggcgtgttct
    11341 gattcttcaa tgcctgagag cattcgagct atctcaatct cacgctcttg atcacttaag
    11401 gatttgattg aagtagatga taaaagaccg ttgctatctt tctcaaccaa gatgtggtta
    11461 tcagcccaga gcgcaacctg cggcagatga gtcacgacaa ttacttgtga attcatagca
    11521 agttttctga gcctctttcc aacttcaagt gctgctttac caccaacacc agcatcaacc
    11581 tcatcaaaga catatgtgcc tagtggataa gagccagcta gaactacttc aagtgccaac
    11641 atcaaacgag agagctctcc gccacttgca gcctttgata ctggaagtaa ttccccgccg
    11701 ctatgagcgg taaagagcat gaagacttca tctagcccaa agagtgagaa atgatcatca
    11761 ctacttccat ctctactttc gactcttact tcaaatattg cctttgccat tgcaagttcc
    11821 actaactctt tagtgacaga tgaggagagc ttttttgcgg attgcgctct aagtgcgctt
    11881 agcgtctttg ctgcttctaa aagctttgct cgcatcccct ccagctctct gcccatttca
    11941 gctaaacgat catctccccc acttaaatcc tttatacgat tcttcgctcc ctctgcctta
    12001 tcaattgcct cagctagcgc ttcgttctta tcgcttgatt tgccatactt tttggcaaaa
    12061 gagctcaagg cagctctacg tgattgcgcc aactccagcg cttgaggatc agcagagagt
    12121 gaatctaaat accgatcaag atctgaggct gcatcaatca aatcaaagag tttctctgaa
    12181 atggcatctg caatttggtc taattgagga tctcgcccct tggcacctgt tagagatttc
    12241 ttagcgctat gcaatgaatt tagagctcct gcctcttcat ctgagagagc ttgctgggct
    12301 ccaagtgagg caatccttaa atcctcaacg ctttcaagct ttgaaatctg agcctccagc
    12361 tgggatagct cggagctttg tggctttaat ttagcaaagt catttacgag ctcttcaagt
    12421 gatttaatct cgctatctct atcaccaaga gctttcttta gttctgagat ctctctacgt
    12481 agcacctggt agttcttaag cgcctcttga taagcaagga gagctgtaga aagtccttga
    12541 ccagcaaata tatctagaat ctctcgctgc ttatttgcct ttgttagtac taaattggca
    12601 tgctggccat gaatttcaat tagctgatct gcaaaagata ttagcgtgct agcagttgta
    12661 gcggagcccg atagttgagc ttttgatttt ccatctcttc ccaattgtct tgaaagaagg
    12721 attgctgatt cctctatctc aggctcatgt tcttgaatga gtacctttaa atcttcactg
    12781 attttctctg gcaatctaaa acgaccgctt accaccgctc tctcctgacc ttgtctgatg
    12841 taatcagcat cggccttctg tcctaagatc aaggagaggg catttagaag cattgtttta
    12901 ccagctccag tttcgccagt aattacattg aagccctctt taaactcaat cgttgcgcta
    12961 tcaattagcc caagtgagcg gatattgatt tcttctaaga gtgagccttt acgctcgccc
    13021 tttttactca ccgcgccagc catcaaccgg taatttaaac ttggcaacaa ggcgatcagt
    13081 aaatggcgct gcatcaatat gggctaggtt tatatagctt ttatcggagg ttagaacaac
    13141 gcgatccttt tctttcaagg caaacttgcg aagaccatca gctgataaaa cgccctcatc
    13201 actttcaaca tcgatggcaa tttctgaatc tgcgccaata accatcggat ctgagaagag
    13261 cgcatgagcg gcaagtggca gaagaactaa ggcttcaacc tctggccaaa cgactggacc
    13321 gcccgcgcta aaggcataag cagttgatcc tgttggcgtt gcacaaataa ctccatcaca
    13381 gccccatctt gaaagtggtc tgtgatcaac ttgaacaaag agctcaatca tcgcctcagt
    13441 acttcgctca actgttacct catttagcgc ccagccttta gtaactaact catcgcctct
    13501 aaatacttga tatgccagcg tcatacgctt ttcagtctta tatttcttat ctctaatggc
    13561 cgagataatt tctgtctgac ttggtttgcc aatctctgcc agaaaaccaa catgtcctag
    13621 attaattccc agcactggga tgttagagcc gtgtatctgt tctgccccac gcaaaatagt
    13681 tccatcgccg ccaagaacta aaaccagttc aatctcgcca tatctctgcc ttacttgctc
    13741 gcttgcaact tgagtgacgc cagatattcc agtatccgca cttgagataa agctaaaacc
    13801 ttctgcgcta agagttttaa caatagatcc agcaagctct tttgcttcag ggcgagttgg
    13861 atgaaggaca agtagaactt cacgctttgt catctactgc ggtcctttcg ctatcgccag
    13921 atctaattct ttatctgaaa tcatctctgc tccagattta agccagagaa aatattcaac
    13981 atttccagaa ggtcccggaa ggcagctggc cactgctcca acggtaccca accctgcgtc
    14041 aaaggcgctc cttgcaatat ccctaactgc agcctttcta agctctggat ctcgcacaac
    14101 tcccccatct gtcaatcgct ctcgcccaac ttcaaattga ggcttaacca taaggagaaa
    14161 atctgcttcg ctcttgctca ctgagagcag agcggggata actagggcta gtgaaataaa
    14221 ggagagatca gcgaccacta aatcaacgct ctctttgatg atttcgggac ttagagtgcg
    14281 cacattggtg cgatcatgta tctcaactct tggatcgttt tgaagctccc aagcaagttg
    14341 tccatatccg acatcaacag cgatgacctt tgctgcccct cgctttagta agacatcggt
    14401 aaatccaccc gttgacgctc cagcatcaag agcggtaacc ccgctaactc ttatctcttt
    14461 aaaggcatcg agtgctccgg ctaatttata cccacctctt gaaacataat cctctcgctt
    14521 gccggcaagc gttatagagg tctctggatc gacctgcgtt gctggctttg tagctggaat
    14581 tccccgaact agaactgatc ttgactcaat tagatcaacc gcatcttctc tagatcttgc
    14641 aaggcctcgt cttactaact cagcatctag acgagttctc attaatcttc ctgcagttct
    14701 agatagatgc ggtggtcaga atttttttaa gattgactct ggctagaaat accttcaaca
    14761 tgagttagcg ccgctgataa atcttgatta acttgatcaa atagcgcgcc atgctcggcc
    14821 aaaggagcaa aggcaatctc atttattctc ttcttgatag ctgagatctg ctctagattt
    14881 tcaatatttt cactcatttt ttcttactcg cactcttttt tgcagcgctc ttctttgctg
    14941 gggctttctt ctttgctggc tttgaagccc ttggagtagt aacaagcttt gcttttaata
    15001 gttcaacttc tcttgccagt cgatctaagt cttcttgctt tgcaaatccc atctttttaa
    15061 cacttgtctt tacctcttct tcaacgcgag ttttaattgc ctcgccactt tccttcgccc
    15121 attgattcaa ggctgcagct atctcctgcg gactcttctc ccctgatgag agcttggtga
    15181 gataggtgcg aagtgctgat aagagatcat ttgccatagg agaacgatag ccaattggct
    15241 agctcttagc cctttgattc ttcagagggt tcaatctttt gatacttaac tctaaattcc
    15301 tcagcctcac ctgcgcgccc ggctgcaatt aaggcctgga tatatgcgcc ttgaagtttg
    15361 gcagcaaagc tggcatcgct aagtgttagc tctggacagg ttagagtcac aacagcggca
    15421 tcactttgac caagagcgct tcttgctcca gcagcggcaa ttcgaagctc tactcgctcg
    15481 ccttgagtta gtaatttggg atcgatagag ccagcaattt caagggtttt tcggggattt
    15541 cccagggcta gctctgcttg ggcaagatat ggcagagcag attgagagcc tgaaattctt
    15601 atcgccgctc ttaactcttt ttgagcaatc tcatatttct gaaaattcag cgcagcaatt
    15661 ccagcccgct ctctaacaat tgccactcgc cctgctctaa atactgctgc ctggccatgc
    15721 caaaatgcac gctctggatt actttcaaga aatagattta tgcagactaa atgctttgca
    15781 acagcttctc cgttttcagc agcgagtgtg ccgagtgcaa atgcatctct cttctctagc
    15841 tcttttccag ttacttctat tggaatttca ggttctgcaa attttcttac tcttctgttt
    15901 tcatcacgta agtcatttct ctcacgacca acaccgcctc ttgcaggtct tggaccgctt
    15961 ctatctgagc ctctgcgatc acttggcttt ctatcgcttg gttttctatc gcttggtttt
    16021 ctatcgcttg gttttctgga agaaggcctg cgatcagagc tcttgttata ggaaggacgt
    16081 gcgggtcttt caaccatttg ctcctcctta accaatatct tgaattctat gttagagatg
    16141 aaaagaaaaa aggggccgca gtaaactgca acccctcgtt tctaatataa gtccggcggc
    16201 gttctactct cccacaaggt gacccgtgca gta
//
LOCUS       DAOROD010000023        14916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_179_length_14916_cov_32.253415, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000023 DAOROD010000000
VERSION     DAOROD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_14916_cov_32.253415"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2940
                     /locus_tag="PLW53_05085"
     CDS             <1..2940
                     /locus_tag="PLW53_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRD36827.1"
                     /translation="MLNIAHLMSISLDGQWNFQLLDRADQDPSKRWQSITVPGLWTMV
                     DGEQPFGDKPIYTNTQMPFDQLPPSVPLDNPTGVYEREFSLPSSWKNKRVVLSIGGFE
                     SLAILTINGKEVGVAKDSRLASEFDITDFITNGSNKVQIRVIKWSDSTFIEDQDQWWH
                     GGITRSVKLFATEYVFIERLYATPGLEKNNKVGTLKIRAHINSINEEEIAGYRLKVKV
                     IGVKGAEASATVKNQKAPNWTQKSATEKQAGDDFFLGTYWDGNMPKDKYQAYLATEPI
                     IPGPLELNIRVPNISPWSAESPSLYDVEYQLIDPSGQVIEVTTQRIGFRSVEIKGCDL
                     LVNGERVMIYGINRHDFNRRSGRVLSRDLMRQDLLELKRWNFNAIRTSHYPNDPAFLD
                     LCDELGFYVVGEANIESHAFQNTLCDDQKYLNAWVDRVARMIQRDIHHASVILWSLGN
                     ESGSGINHRAAAAYARSFDPTRPLHYEGAIRGNWTTGHDITDVVVPMYPDISAIISYA
                     KSKKADRPLIMCEYSHAMGNSNGTLAEYWQAIHSLPALQGGFIWEMWDHGLDQRLEDG
                     SIRSAYGGDFGETKHDGNFCCDGMFFPDRSPKPALSEFKYIASPIEIKAKNLKSGRFE
                     IFNKNFFVNLSDYKLKYEVTVNGKASSSGEVKLGLVKPRQSKPFTLPAQVLKGDGSVG
                     ERFINFSLTLASSKPWAHIGHEVAWEQIALISKPLPKIKSAKGKMQYVNSQGQIQVPF
                     GQIAPALTLWRAPTDNDLIGHISQRWDEWGVRKLELESSKVVRTSTNTKITNLWRAQG
                     GAAIKHIQLVESVDSGFRVSETVTLPKELDDLARVGINFEVDGALNNFTYFGIGPNET
                     APDRKIGKVHRYSSTVDQQYVPYVKPQENGGHMGMRWFSLTNQSGHGLYFQLDKPRMV
                     TVSPMRSEDLANATHNVFVNPCGNTVITIDGAHRGIGTASCGPDTLAKYLIKPGTYKW
                     SWSVVTF"
     gene            complement(2937..3713)
                     /locus_tag="PLW53_05090"
     CDS             complement(2937..3713)
                     /locus_tag="PLW53_05090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRD36828.1"
                     /translation="MSYNRFIALGDSMTEGMQDEKIKGNYRGWADRVADVMASNYENF
                     TYANLAIRGKKVGQVLREQVPVALGLVNGPSTIISFHAGANDVIRPKYDPVKTFAEYD
                     QAVRALTKSGATVMLFCVLEDSGAKTRAAKVWKLRFEAFNANVRKIASEVGAILLDPN
                     QESSWRHPGFIHEDRLHLNSLGHYRVAQAVLARLNLPHDPSWRTPLPPPVKLPLAEQI
                     KQNTRWIFLYGIPWAIRRIRKKSSGDGRSPKYPAPITWKP"
     gene            complement(3723..4679)
                     /gene="rlmB"
                     /locus_tag="PLW53_05095"
     CDS             complement(3723..4679)
                     /gene="rlmB"
                     /locus_tag="PLW53_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRD36829.1"
                     /translation="MAKPDRKPPKKSTRSLRGKGPTPKAEDRPYHKKYRSKEDSARKR
                     TERKRIDSKRGPKGDKKFAQRRDFRRNEREHIDTVAGRNAVLEALRAGIPAKELILAV
                     GVDVDERLNESIRLAQKMAIGIKEVERRAIENMTGIANHQGIALVVKPFQYSSQKEIF
                     ARAKDPALFIAVDGVTDPRNIGAIARSAAAFSADGLLIPERRNASLTAAAWKSSAGAA
                     ARLPIAQITNLARSVEDAKAYGCFIVGLDGDADTSVEAMEIADQPLYLIVGSEGKGLS
                     RLIREKCDLLVSIPMSSAVESLNASVAVSIALYAVDRKRAIK"
     gene            complement(4672..6072)
                     /gene="cysS"
                     /locus_tag="PLW53_05100"
     CDS             complement(4672..6072)
                     /gene="cysS"
                     /locus_tag="PLW53_05100"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRD36830.1"
                     /translation="MADMKKLRLYDTASRKVSEFKPIVAGQASIYLCGATVQASPHVG
                     HVRSGINFDILRRWLIASGYNVTFIRNVTDIDDKIIHNAGHEDMPWWALAMKYERSFS
                     DAYRALNVMAPTYEPRATGHITQMIELIEKLITNGSAYAPGNGDVYLEVRKLKSYLTL
                     SNQKLDDLLVAKDGEEKLKRDPRDFALWKGAKEGEPYWPTPWGNGRPGWHIECSAMAH
                     AYLGQVFDIHGGGLDLIFPHHENEIAQSEAAGYEFARLWMHNAWVTTSGEKMSKSLGN
                     SLKVDEVLKKVRGIELRWYLSSAHYRSMLEFSFEALDEAVVSFKRIENFLNRAKELLG
                     SAPGMEISQELSDALNDDLAVPQVLAQISEWLKLGNSALSASDKAQVAKYASYIRAAL
                     SIIGTDPFDSAYSSSSSADLTGAVDGLISLLLEQRSAARARKDFAAADQIRDQIIAMG
                     ISIEDRADGVRWSING"
     gene            complement(6074..7222)
                     /gene="ispD"
                     /locus_tag="PLW53_05105"
     CDS             complement(6074..7222)
                     /gene="ispD"
                     /locus_tag="PLW53_05105"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRD36831.1"
                     /translation="MSQLPKSAAIIAGAGMGHRLGAEIPKALIQIQGITLLERAFISL
                     SKVVDEIIITAPDGYEEQFKAIVGQSAEVITGGVLRSDSIRVALKALSPSVESVLIHD
                     AARALASSALAARVLGDVQSGQSAVIPVLKVIDTIKEIDRDGFVRATPDRSSLVAAQT
                     PQGFNRQVLERAHSASDDATDDAALVEAIGVKVKTIEGEASAFKITTKEDIGQALLLL
                     SPIDRNFKVGLGVDAHAFSSDQDRKLWLAGLLWPEQIGVDGYSDGDVAAHAICDALFS
                     AAGLGDLGSNFGVDDPKYQGASGETLLKEAFGKITSAGYEIGNVSVQIVCNKPKIGPR
                     RAEAIKALSKALGGAEVSVSATSTDGLGFTGQGLGISALATALLIEKK"
     gene            complement(7246..7728)
                     /locus_tag="PLW53_05110"
     CDS             complement(7246..7728)
                     /locus_tag="PLW53_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRD36832.1"
                     /translation="MSFKVGETVVYPHHGAALIEAIETRTIKGEEKIYLVLKVAQGDL
                     TVRVPAENVDLVGVRDVVDSSGLERVFGVLRQPYTEEPTNWSRRYKANLEKLASGDVI
                     KVAEVVRDLFRRDLDRGLSAGEKRMLAKARAILISELALAEHTDEAKAESILDEVLAS
                     "
     gene            7829..8287
                     /locus_tag="PLW53_05115"
     CDS             7829..8287
                     /locus_tag="PLW53_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36833.1"
                     /translation="MKSRAIATMIVASFFLTGCAAGGNAPTRLIKQVTDGVEKDIGDL
                     KIRNVKIIALPDGSGTLIGFIVNHSDEVDQLVAITVGGQRADFLSDVQLLKNKPMIFE
                     GESANAKAKIAALGVKPGYRVPVVLYFAKNGKVELDALVVANTGIYSSIL"
     gene            complement(8269..8952)
                     /locus_tag="PLW53_05120"
     CDS             complement(8269..8952)
                     /locus_tag="PLW53_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD36834.1"
                     /translation="MTKILIVEDEPSFSEALEFLLGKEGFAVVTAENGSDALKKFEQG
                     GIDLVLLDLMIPEVSGTEVCRQIRAKSKVPIIMLTAKDSEVDKVVGLEIGADDYVTKP
                     YSARELVARIRAVLRRNSGELGESDTGVMTVASVRMDIERHQVSVNGIPISLPLKEFE
                     LLEFLMRNAGRVLTRGQLIDRVWGSDYVGDTKTLDVHVKRLRAKIENDPANPVLIQTV
                     RGLGYKMEL"
     gene            complement(8949..10016)
                     /locus_tag="PLW53_05125"
     CDS             complement(8949..10016)
                     /locus_tag="PLW53_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD36835.1"
                     /translation="MKFRRQEGENLEAPEVLPQVLLKTLQSLESESLIIIPGEQILFQ
                     SPGITNFAIVKDERITSEDLMALIRVARRTNETHRSRIEIARGPIGAGKREFEVSVSP
                     LNDEGMVLVLISDQSEARRIDAVRRDFVANVSHELKTPIGALGLLSEAILGAKDQPDA
                     VVKFASRMQMEAKRLTDLVQEIIDLSRLQSSDPLQKAFDVEASDVVREAVAQSAFSAE
                     SRKITVEIGEIEDATVIGDRDQLIMAVLNLVENAINYSPENTKVSVVVKVKEELLEIS
                     VTDQGIGIAEGELARVFERFYRVDPARSRMTGGTGLGLSIVKHVALNHGGDIKVWSKE
                     GVGSTFTLQLPIARSNEGELS"
     gene            10143..10802
                     /gene="phoU"
                     /locus_tag="PLW53_05130"
     CDS             10143..10802
                     /gene="phoU"
                     /locus_tag="PLW53_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRD36836.1"
                     /translation="MSWLRTAFQDELDGVTQTLSELSSLVSSAITQATHALLTADLSE
                     AEAVIAADDRVDEIQHELDARIIDIIARQQPVASDLRALVTALRMSADLERMGDMAHH
                     IAKITRLRHPGAAVPSELLLTIEEMGKVARLISDKVGGVINSKDIDKALEVEKDDDEM
                     DLLHRRLFTALLEPSWPHGIETAIDMTLIGRYYERYADHAVSVSRRIHFQVTGKYNNE
                     S"
     gene            complement(10803..11549)
                     /locus_tag="PLW53_05135"
     CDS             complement(10803..11549)
                     /locus_tag="PLW53_05135"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HRD36837.1"
                     /translation="MSIGKLILLRHGQSQWNAKNLFTGWVDVALSSQGEAEAKRAGEL
                     LKEKNLLPDVLHTSLLTRAIHTAEIALGACERKWIPVSRSWRLNERHYGALQGKDKAA
                     TLKEYGQEQFKLWRRSFDTPPPAIADSDQYSQAGDIRYKDLGPDLPKTECLKDVVKRL
                     MPYLDNEIAKDLRQAKTVLVVAHGNSIRAIVKHLACISDSDIAEVNIPTGIPLLYRFD
                     DDFEPIIKEGEYLDPEAAKESIAAVANQGK"
     gene            complement(11571..12113)
                     /locus_tag="PLW53_05140"
     CDS             complement(11571..12113)
                     /locus_tag="PLW53_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRD36838.1"
                     /translation="MSMIKFSHGALKNHALLLDMTTRAMASATVEEFLDSHEIDYEKS
                     GANTYLLTLPGQSKLETHCALVVGDHSLSINAFVIRKPDENIAAVHNYLLTKNANMYC
                     LAFAINELGDIFLVGRLALSAVSENELDRIIGAVLQYSDSAFNPLLELGFSSAIRREW
                     AWRLSRGESLSNLKAFEHLI"
     gene            complement(12058..13329)
                     /gene="mshA"
                     /locus_tag="PLW53_05145"
     CDS             complement(12058..13329)
                     /gene="mshA"
                     /locus_tag="PLW53_05145"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HRD36839.1"
                     /translation="MAKQLRIASLMLHTSPLEQAGIGDAGGMNVYVVESAKRIAESGI
                     GVDIYTRANRSGLPEVVEIADGVSVRHLEAGPYGGITKDELPSQVCALMSSFMHQEAR
                     LASNYYELVHSHYWISGQLGWLISERLSIPLVNNMHTMAKVKNLALAEGEKAEPEIRA
                     IGEEQVVKASSALIANTASEAASLVSLYDACPDRVFVVPPGVDLKTYKVGAGKSAARA
                     KLLIKPETLLLAFVGRIQPHKGPDVLIRAVAEMLKHTPSLKSRLKAVIIGGASGSGQG
                     EIEKLLGLARFLGVSEVFEFLPPVAHEDLSDWYRASDLVCVPSYSESFGLVALEAQAC
                     GTPVVATAVGGLRTAVADGISGLLVDGHDPRAWSATILRLLLEPEKRLLLSMGAVEHA
                     SHFGWDSTARGILDVYDQVLSRGIKKSRALA"
     gene            complement(13329..14258)
                     /locus_tag="PLW53_05150"
     CDS             complement(13329..14258)
                     /locus_tag="PLW53_05150"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRD36840.1"
                     /translation="MNSGEVLLEVVRNDLVESIHSGHLLILDSDGREVLALGELDQLI
                     YPRSAIKSLQASAMLRAGLKLNEQQIALACASHAGSDAHLEVVRSTLASVGLDESALQ
                     NTPDKPLGAFERAAWVGSPSSLAANCSGKHAAMVATCKTNNWDLASYKEPSHLLQLAI
                     ANEFEILSGEQITHVAVDGCGAPLFALTLRGLARAMRSLALSADPIHKTVIRSCINNP
                     VLVSGINRLPTLLMQRVDGLFVKDGAESVMVMATSNGEVIVWKISDGSQRGAGELARA
                     SLSHLGINLDIEPEYVLGGGEVVGEIRASKLLI"
     gene            complement(14264..14761)
                     /locus_tag="PLW53_05155"
     CDS             complement(14264..14761)
                     /locus_tag="PLW53_05155"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HRD36841.1"
                     /translation="MAFQIPEGLHPDLNPLAWLVGSWSGKGHGEYPGIESFQFAQEVT
                     FAHDGRNFLTYFSRSWIIDENNEIVQTASSETGFWRVKPDNQLEVLISHSTGLAEGWV
                     GRFDGPKIQLAMDHAYSAPSAKAIEGGQRLYGLVDGELFYAYDMAFNGHKLQAHIWST
                     LPRIS"
BASE COUNT         4102 a         3534 c         3056 g         4224 t
ORIGIN      
        1 atgctcaata tcgcgcatct gatgagtatt tctcttgatg ggcaatggaa ctttcaatta
       61 ttagatagag ctgatcaaga cccaagtaag cgctggcaga gcatcacagt tccaggcctt
      121 tggaccatgg ttgatggcga gcaacccttt ggcgacaagc cgatctatac caatactcag
      181 atgccctttg atcaactgcc accaagtgtt ccacttgata atccaactgg agtttatgag
      241 cgagagtttt cacttccctc atcttggaaa aataaaagag ttgttctatc aattggagga
      301 tttgaatccc ttgcaatcct taccataaat ggcaaagagg ttggggttgc taaagactct
      361 cgcctagcat cggaatttga tattacagat tttattacta atggttcaaa taaagttcag
      421 atcagagtca ttaaatggtc tgattcaaca tttattgagg accaagatca atggtggcat
      481 ggcgggatta caagatctgt aaaactcttt gctaccgaat atgtttttat tgaaagactc
      541 tatgccaccc cagggcttga aaaaaataat aaagttggaa ctcttaaaat cagagctcat
      601 atcaattcaa ttaatgaaga agagattgct ggataccgac ttaaggtaaa ggtcattggc
      661 gtaaagggcg ctgaggcaag cgcgacagtt aaaaatcaaa aagcgccaaa ttggacgcaa
      721 aaaagtgcga ctgaaaagca agcaggagat gatttctttc taggcaccta ctgggatggc
      781 aatatgccta aggataaata tcaggcatat ctagcaactg agccaataat tccagggcca
      841 ttagaactaa acattagagt gccaaacatc tccccttggt ctgctgaatc gccaagcctt
      901 tatgacgttg agtatcagtt aattgatcct tcaggccaag taattgaggt cactacgcag
      961 agaattggct ttagaagcgt tgagataaaa ggatgcgacc tgctagtaaa tggcgagcgc
     1021 gtgatgatct acggcatcaa tcgccatgat ttcaatcgca gaagcgggcg agtattaagc
     1081 cgggatttga tgcgacagga tttattggaa cttaagcgat ggaactttaa tgccatccgc
     1141 acctcgcact acccaaatga tccagccttt cttgatctct gcgatgagct tggtttctat
     1201 gttgtaggtg aggccaatat tgaatctcat gccttccaaa acacgctctg tgatgatcag
     1261 aaatatctaa atgcttgggt tgatcgagtt gccagaatga ttcaacgaga tatccatcat
     1321 gcatcggtga ttctctggtc tcttggcaat gaatcaggct ctggaattaa tcatcgagct
     1381 gcagctgcat atgctagaag ttttgatccc actcgcccgc tgcattatga aggagcaatc
     1441 agaggaaatt ggactacagg acatgacatc acagatgttg tagttcctat gtatccagat
     1501 atctcagcga ttatttctta tgcaaagtct aaaaaggcag atcgccctct aattatgtgc
     1561 gagtactccc acgccatggg aaatagcaat ggaaccttgg ccgaatactg gcaagcaatt
     1621 catagccttc ccgctcttca aggcggcttt atttgggaga tgtgggatca cggacttgat
     1681 caaagacttg aagatggaag tatccgctcg gcctatggcg gcgactttgg tgaaactaaa
     1741 catgatggca acttctgctg cgatggaatg ttctttcctg atcgaagccc aaagcctgcc
     1801 ttaagtgaat ttaaatatat tgcctcgcca attgagatta aggcaaagaa tttaaagagt
     1861 ggaagatttg agatttttaa taagaacttc tttgtaaatc tctcagatta caagcttaaa
     1921 tatgaggtaa cagtcaatgg aaaggcctcc tcatctggcg aggtaaagct agggctagta
     1981 aaaccccgtc aatcaaagcc ttttaccctg cctgctcaag tattaaaggg cgatggatca
     2041 gttggtgagc gctttattaa cttctcgctc acacttgcta gtagcaagcc ttgggctcat
     2101 attggccatg aagtcgcctg ggaacagata gcacttattt caaagccgct accaaagata
     2161 aagagcgcta agggcaaaat gcaatacgtt aattctcaag gccagattca agttccattt
     2221 ggacagatag ctcctgcgct aacgctctgg agagctccaa ctgataatga cttaattggc
     2281 catatctccc aaagatggga tgagtggggc gttagaaagt tagagctaga gagctcaaaa
     2341 gttgtgcgca cttcaactaa taccaagata acaaatctct ggagagctca aggtggagct
     2401 gctattaagc atatccaact tgttgaaagc gttgattcag gatttagagt tagtgaaact
     2461 gtaactcttc caaaagagct agatgatcta gcaagagttg gtatcaactt tgaagtagat
     2521 ggagcgctca ataactttac ctattttggt attgggccca atgaaactgc tccagataga
     2581 aagattggca aggttcatcg ctatagctca actgttgacc agcaatatgt gccatatgta
     2641 aagccacagg aaaatggcgg gcatatgggg atgcgctggt ttagcctgac taatcaaagc
     2701 ggccacggac tctatttcca actagataaa ccaagaatgg tcactgtctc tcctatgcgc
     2761 tctgaagatt tagcaaatgc cacccataac gtatttgtta atccttgcgg aaatacagtt
     2821 atcactatcg atggcgcaca tcgcggaatc ggaactgcct cttgcgggcc agatactctt
     2881 gctaagtact taattaagcc tggaacttat aaatggagct ggagcgtagt taccttttaa
     2941 ggcttccaag taattggcgc tggatatttg gggctccggc catctccaga tgacttcttt
     3001 ctaatccttc ttatcgccca gggaatgcca tagagaaaga tccatcgagt attttgtttg
     3061 atttgctctg ccaaaggcag ctttactggc ggcggcagcg gagttctcca acttgggtca
     3121 tgaggaagat ttagtcgagc aagtactgct tgagcaacac gataatgtcc aagagaattt
     3181 aagtgaaggc gatcttcatg gataaaacct ggatggcgcc aggaactctc ttgatttgga
     3241 tctagcaaga tagcgccaac ttcgctggct atctttcgca catttgcatt aaatgcttca
     3301 aatctcagct tccaaacctt tgctgcgcga gtcttagcac ctgaatcctc taggacacaa
     3361 aagagcataa ctgttgctcc acttttagta agtgctcgta ctgcttggtc atactctgca
     3421 aaggtcttaa ctggatcata ctttggtctt attacatcat tggctccagc atgaaaagaa
     3481 ataattgtgc ttggaccatt aactaaacca agtgctactg gaacttgctc tcttaatacc
     3541 tggccaactt ttttacctct tattgctaga ttggcatagg tgaagttctc ataattgctt
     3601 gccataacat cagcaacgcg atctgcccag ccacgataat tgccttttat cttctcatct
     3661 tgcataccct cagtcattga atcaccgagt gcgataaagc ggttataaga cattgttaac
     3721 ctctacttaa ttgctctttt tctatcaact gcatagagcg caattgatac ggcaacgctg
     3781 gcatttagtg attccaccgc gctactcatg gggatagaga caaggagatc gcacttctcg
     3841 cgaataagcc ttgataaacc tttaccttcg ctgccaacga tcaaatagag cggctggtca
     3901 gcaatttcca tcgcctcaac gctggtatca gcatcgccat ctaggccaac gataaaacag
     3961 ccataagcct tagcatcttc aacagatcta gctaagtttg ttatctgagc aattggaagt
     4021 cttgccgcag ctcctgccga gctcttccaa gcagcagcgg tgagtgaagc gttgcgacgc
     4081 tctggaataa gaaggccatc tgcgctaaat gctgccgcac ttcttgcaat tgcgccaata
     4141 tttcttggat ctgttactcc atcaactgcg ataaagaggg caggatcttt agctcttgca
     4201 aaaatctctt tctgagatga atattgaaat ggcttaacga caagtgcaat gccttgatga
     4261 ttggcaatgc cagtcatatt ctcaattgcg cgtctttcaa cctctttaat tccaatcgcc
     4321 atcttctgag caaggcgaat agattcatta agtctttcat caacatcaac gccaactgcc
     4381 aagatcagtt cttttgctgg aataccagcg cgaagcgctt caaggacagc atttcttcca
     4441 gcaacggtat ctatgtgctc acgttcattg cggcgaaagt cgcgccgctg cgcaaatttc
     4501 ttatcgccct tagggccgcg cttggaatca attcgttttc tctcagtacg ttttctcgct
     4561 gaatcttctt tagatctata tttcttatgg taggggcgat cttcggcttt tggagttggc
     4621 cctttgcctc taagcgatct tgttgatttc tttggtggtt tacgatctgg cttagccatt
     4681 gatactccat ctcacgccat ctgctcggtc ttcaatggag attcccattg cgattatttg
     4741 atcacggatc tgatctgctg cagcaaaatc ttttctagct cttgccgcac ttctctgctc
     4801 caatagtaat gaaataagtc catctactgc gccagttaaa tcagcgctag atgagctgga
     4861 ataggctgaa tcaaaggggt cggtaccaat aattgatagg gcagccctga tatagctcgc
     4921 atattttgct acttgagctt tatcagaggc gctaagtgcg ctatttccaa gctttagcca
     4981 ctctgaaatc tgcgccaata cttgaggcac tgctagatca tcattgagcg catcacttaa
     5041 ctcttggctg atctccatac ctggagcgct tcctagtaac tctttagccc tatttaaaaa
     5101 attctcaatt cgtttaaagg agactaccgc ttcatcaagt gcctcaaatg agaattccag
     5161 cattgagcgg taatgagcac ttgatagata ccaacgaagt tcaatgccgc gaactttctt
     5221 taatacctca tcaaccttta gtgagtttcc aagagattta ctcatcttct cgccagatgt
     5281 tgtcacccag gcgttatgca tccaaaggcg agcaaattcg tatcctgctg cctcagattg
     5341 ggctatctca ttttcatgat gtggaaaaat taaatcaaga ccgccgccat gaatatcaaa
     5401 gacttgccct agataagcat gagccattgc agagcactca atatgccaac ccggtctgcc
     5461 attgccccaa ggtgttggcc aatatggctc tccttctttt gcacctttcc agagcgcaaa
     5521 gtctcttgga tcgcgcttta atttctcttc gccatcttta gcaactagta gatcatctag
     5581 cttctgattt gaaagggtta gataagactt taacttgcga acctcaagat agacatcgcc
     5641 atttcctgga gcgtaggcag atccattggt aatcaatttt tcaattaact caatcatctg
     5701 agtgatatgg ccagttgccc ttggctcata ggttggggcc atcacattta gagctcgata
     5761 agcatcgcta aatgagcgct catacttcat ggcaagtgcc caccaaggca tatcttcatg
     5821 gcctgcgtta tgaattatct tgtcatcaat atctgtaaca tttctaataa atgtcacgtt
     5881 atagccagag gcaattaacc agcggcgcaa aatatcaaag ttaattccac ttctgacatg
     5941 gccaacatga ggagatgctt gaaccgttgc tccgcataga taaattgaag cctggccagc
     6001 aactattggc ttaaattctg aaacctttcg tgatgcggtg tcatagaggc gaagcttctt
     6061 catatctgcc atcttatttc ttctcaatta gaagcgcagt tgcaagagct gagatgccaa
     6121 gtccttgtcc agtaaaacct aggccatctg ttgaagtggc acttactgaa acctcagctc
     6181 cgcctaaggc ctttgaaagt gctttgattg cctcagctct tcttggacca atttttggtt
     6241 tattgcaaac tatctgcact gagacatttc ctatctcata tccagcactt gttatcttgc
     6301 caaatgcctc ttttaataac gtctcaccgc ttgctccctg atactttgga tcatctacgc
     6361 caaagtttga accaagatcg ccaagtcctg ctgcgctaaa gagcgcatca cagattgcat
     6421 gtgctgcaac atcaccatcg gagtatccat caacgccaat ttgttcgggc catagcagcc
     6481 ctgcaagcca taattttcta tcttgatcac ttgagaatgc atgggcatca acgccaagtc
     6541 ctactttgaa atttctatct atagggctta gcagcaagag agcttggccg atatcttctt
     6601 ttgtggtgat cttaaaagct gaagcctccc cctcaattgt cttaaccttc actccaattg
     6661 cttcaacaag ggccgcatca tcggtggcat catctgaggc gctatgagca cgctctaata
     6721 cttggcgatt aaagccttgc ggggtttgtg ctgcaacaag tgatgagcga tcaggtgttg
     6781 ctcgaacaaa accatctcta tcaatttctt tgatggtatc gattactttt aagactggaa
     6841 ttactgcgct ctggcctgat tgaacatcac ctaaaactct agctgccaga gcgctacttg
     6901 caagggctct tgcagcgtca tggattagaa cgctctcaac gctaggcgat aaggctttta
     6961 gagcaactct tatcgaatca cttcttaata ctccgccagt tattacctct gcgctctggc
     7021 caacaattgc tttgaattgc tcttcatatc catctggcgc agtaatgatg atttcatcaa
     7081 caactttaga gagcgatata aatgcgcgct ctaataaagt gatgccttga atttgtatta
     7141 aagctttggg aatttcagca cctaatctat ggcccattcc tgcgccggcg attatggcgg
     7201 cgctttttgg taactggctc ataagaccct atcgagggga gtgctttaag aagctaatac
     7261 ctcatcgaga attgattcag ctttagcttc atcagtgtgc tcggcaagtg ctaactcaga
     7321 aattaaaatc gcacgcgctt tagcaagcat gcgcttctcg ccggcagaga ggccacgatc
     7381 tagatcgcga cggaatagat cacgaactac ttcagcaact ttaataacat cacctgatgc
     7441 tagtttttca aggtttgcct tatagcggcg agaccaattt gttggctcct cggtatatgg
     7501 ctggcgcaaa actccaaaga ctctctcaag acctgaggaa tccacaacat ctctaacgcc
     7561 aactaaatca acgttttctg ctgggactcg cactgttaga tcgccttggg cgacctttaa
     7621 aactaggtag atcttctctt cgcctttgat ggtccgggtc tcaattgctt ctatgagagc
     7681 cgctccgtgg tgggggtaaa caacggtttc gccgacctta aatgacattt tttgcctttc
     7741 gctggaagag tattttatca gagttatctc aatcacgaaa accagcaaaa gtaagcgtta
     7801 gataatcact ttctgccatt attgcccaat gaaatcaagg gctatcgcaa cgatgattgt
     7861 cgctagcttc tttctcacag gttgcgcagc tggtggaaat gctccaactc gattaatcaa
     7921 gcaagtaacc gatggcgttg aaaaagatat tggcgattta aagattcgca atgtaaaaat
     7981 cattgcactg cctgatggaa gcggaaccct tatcggattt atcgtcaatc acagcgatga
     8041 ggtagatcaa ttagttgcaa ttacagtggg cggacagaga gctgatttct taagtgatgt
     8101 ccaacttctt aaaaacaagc cgatgatctt tgagggagag tcagcaaatg ccaaagctaa
     8161 gattgcagct cttggtgtaa agcctggata tcgcgtgccg gtagttctct actttgcaaa
     8221 aaatggcaaa gttgaacttg atgccctagt tgttgctaat actggaatct acagctccat
     8281 tttatagcca agcccgcgca cggtttgaat tagcacagga ttagcaggat cattttcaat
     8341 ctttgctctt aaacgtttaa catgaacatc aagtgttttc gtatctccaa cataatcact
     8401 tccccagact cgatctatca actgccctct agttaatact cgcccagcat tgcgcattag
     8461 aaactctaat aactcaaact ctttaagcgg cagtgaaatt ggaatgccat ttactgagac
     8521 ttgatggcgc tcaatatcca ttcgcactga agctaccgtc ataactccag tatcactctc
     8581 gccaagttcg cctgagttac ggcgaagcac tgctctaatt ctggctacca actctcttgc
     8641 tgaatacggc ttagttacat agtcatctgc gccaatttca agacctacta ccttgtcgac
     8701 ctcagaatct tttgcagtta acatgatgat tggaaccttg gatttagctc gtatttggcg
     8761 acagacttca gtgcctgaga cttctggaat catcaaatca agtaggacta gatcgattcc
     8821 accttgttca aacttcttta gcgcatcact gccattttca gctgtgacta ccgcaaaccc
     8881 ctctttgcct aagagaaact caagcgcctc tgaaaatgat ggctcatcct caactatcaa
     8941 gatctttgtc acgatagctc cccttcatta gagcgtgcga taggaagctg gagcgtaaat
     9001 gtgctgccaa ctccctcctt actccaaact tttatatccc cgccatgatt tagcgctacg
     9061 tgtttaacaa tcgaaagccc tagaccagta ccgcccgtca ttcgagatct tgcaggatct
     9121 actcgataga aacgttcaaa gactcttgca agttcacctt ctgctatacc tattccctga
     9181 tctgtaacag atatctctag taactcttcc ttaaccttta ccaccaccga aaccttagta
     9241 ttttcaggtg agtaattaat ggcattttct accaagttca ataccgccat aatcaattgg
     9301 tcgcgatcgc caattaccgt tgcatcctct atctcgccaa tttcaactgt tatcttcctt
     9361 gactcagctg aaaaagctga ttgcgccaca gcttccctta cgacatcact tgcttcaaca
     9421 tcaaaggcct tctgcagcgg atcactactt tgcaatctag agagatcgat aatttcttga
     9481 actagatcag ttaatctctt ggcctccatt tgcatcctgc tagcaaattt aaccactgca
     9541 tctggttggt ctttagcccc aaggattgcc tcgctaagta aaccaagggc cccaattgga
     9601 gtctttaact catgagaaac atttgcaaca aagtcgcgtc taactgcatc aattcgcctc
     9661 gcctcactct gatctgagat aagcactaaa accattcctt catcatttag tggcgatact
     9721 gatacttcaa attcacgttt accagctccg attggacctc tagcaatctc aatacgactg
     9781 cgatgggttt catttgttct tcgcgcaact ctaattaacg ccattaaatc ttcacttgtt
     9841 attcgctcat cttttactat tgcaaaatta gtgatccctg gagattgaaa gagaatttgc
     9901 tcgccaggaa taatgattag agattcactc tcaagggatt gcagggtctt aagtaagact
     9961 tggggaagga cttctggcgc ttctaagttc tcgccctctt gcctgcgaaa cttcatgtag
    10021 caatactacg aaaaggggga tagcccgtca tctctatcga cttcacctgt cgttcaccgc
    10081 ctctttcact tttatttacc taacttccgc acttttagcc ttaaatccat taggtttagc
    10141 aaatgagttg gctacgaacg gcatttcaag atgagttaga tggcgttacc caaaccttat
    10201 ctgagttatc cagtttggtc tctagcgcaa ttactcaagc aacccatgcg ctattgactg
    10261 cagacttgag tgaagctgaa gctgttattg ctgcagatga tcgcgttgat gaaattcaac
    10321 atgagcttga tgccagaatc attgacatta ttgctcgcca acagcctgtg gcatcagatc
    10381 taagagcgct agtaactgcc ttaaggatga gcgcagatct agagcggatg ggagatatgg
    10441 cccatcacat cgcaaagatc accagacttc gccaccctgg agctgccgtt ccatcagagc
    10501 tattactaac aattgaggag atgggcaaag ttgctcgatt aatctcggac aaagttggcg
    10561 gagttatcaa ctcaaaagat attgataaag ctttagaagt tgaaaaagat gatgatgaga
    10621 tggatcttct tcatcgaagg ttatttaccg cgctccttga accatcctgg ccacatggaa
    10681 ttgaaacagc aatcgatatg actctaattg gtcgctacta cgagcgctat gcagatcacg
    10741 cagtttcagt ttctagaaga attcacttcc aagttactgg aaaatataac aacgaatctt
    10801 aattacttac cttgattagc aactgctgct attgattctt tagcagcctc tggatctaaa
    10861 tactcgccct ctttaataat tggttcaaag tcatcatcaa atcgatagag cagcggaata
    10921 ccggttggga tatttacctc agcaatatcg ctatctgaaa tacaggcaag gtgtttcaca
    10981 attgctctaa tcgaatttcc atgggctacc accaatactg tctttgcctg ccttaaatct
    11041 tttgcaatct cattatctag ataaggcatg aggcgtttaa cgacatcctt taagcactct
    11101 gttttaggta gatctggacc tagatcttta tagcgaatat cgccggcctg cgagtattga
    11161 tcgctatcgg ctatcgcagg aggcggggta tcaaaagatc ttctccagag cttaaattgc
    11221 tcctgcccat actcttttaa agtcgctgct ttatcttttc cttgcagcgc tccatagtgg
    11281 cgctcattta atcgccaaga tctacttact ggaatccatt ttctctcaca agccccaagt
    11341 gctatctctg cggtgtgaat cgctctagtt agtagtgagg tgtgaagaac atctggaagt
    11401 aaattctttt cctttaatag ttctccggcg cgcttagcct ctgcctcgcc ctggctacta
    11461 agggccacat caacccagcc ggtaaagaga ttcttcgcat tccactggct ctggccgtgg
    11521 cgcagcagga ttaatttacc gatactcatg gccttatctt atcttgagaa tcaaatcaga
    11581 tgttcaaatg cttttagatt cgataaagat tcaccgcgtg ataagcgcca agcccactca
    11641 cgtcttattg cgctactaaa tcccaactcc agcaatggat taaaggctga atcactatat
    11701 tgcagcaccg ctccaatgat tcgatcaagc tcattttcac taacagcgct tagcgcaagt
    11761 ctgccaacaa ggaaaatatc tcccagctca tttatagcaa atgctaggca atacatattg
    11821 gcatttttgg tgagaagata gttatgaact gcagcgatat tctcatcagg ctttctaatc
    11881 acaaaggcat tgatacttag tgagtgatct ccaacaacaa gagcgcaatg agtctcaagt
    11941 tttgattgcc ctggaagggt tagaagatag gtatttgcgc cacttttttc ataatcaatc
    12001 tcatgagaat caagaaactc ttcaactgtg gcgctcgcca tcgctctagt tgtcatatca
    12061 agcaagagcg cgtgattttt taatgccccg tgagagaact tgatcataga catcaagaat
    12121 tcctctagcg gtgctatccc aaccaaaatg tgaggcatgc tcaacggcgc ccattgagag
    12181 cagaagcctt ttctctggtt caagaagtaa gcgcaaaatc gtggcagacc aggctcttgg
    12241 atcatggcca tctaccaata gacctgaaat gccatcagct accgctgttc ttaagccgcc
    12301 aacagcagtt gcaactactg gtgttccaca tgcttgtgcc tctagtgcaa caagaccaaa
    12361 ggattctgaa tatgaaggaa cacagactaa atcacttgcg cgataccaat cagagagatc
    12421 ttcatgggca actggtggaa gaaattcaaa tacttcagat actcctaaaa atcttgcaag
    12481 gcccaataac ttttcgatct cgccctgacc gcttccagat gctccgccaa taattactgc
    12541 ctttagacga gacttaagtg atggagtatg ttttaacatc tcagcaactg cgcggattaa
    12601 tacatctggt cctttatgtg gctgaattct tcccacaaat gctagaagga gagtctctgg
    12661 cttaattaat aatttagctc gcgctgcgct ctttcctgcg ccaactttat aggtctttaa
    12721 atcaactcct ggcgggacta caaagactcg atcagggcag gcgtcataga gtgaaaccaa
    12781 gctggcagcc tctgatgcgg tgttagcaat aagtgcgcta gatgctttta ctacctgctc
    12841 ttctccaata gctcgaatct ctggctctgc tttctctccc tcagcaagag caaggttttt
    12901 aacctttgcc atcgtatgca tattgtttac aagcgggatt gataggcgct cactaattag
    12961 ccaaccaagt tgtcctgaga tccagtaatg agagtgaact agctcgtagt aattagaagc
    13021 aaggcgcgct tcctggtgca taaaagaaga catcagcgcg cagacctgtg agggcagctc
    13081 atctttagta attccgccat aaggcccagc ctcaaggtgt cgaacgctta cgccatcggc
    13141 aatctcaact acctcaggaa ggcccgagcg attagctctg gtgtagatat caacgccaat
    13201 tcctgattct gcaattctct ttgcgctctc aacaacatag acattcattc cgccggcatc
    13261 accaattccc gcttgttcaa gcggtgaggt atgaagcatc aaagatgcaa tccgtaattg
    13321 cttagccatt agatgagaag tttactcgct ctaatctcac cgacaacctc gcctccaccc
    13381 agcacatatt caggctcaat atcaaggtta atacctagat gggaaaggct tgctcttgct
    13441 aactcacctg ctcccctttg agatccatcg ctaattttcc agacaattac ttccccattg
    13501 cttgttgcca taaccattac agattcagcg ccatctttaa caaataagcc atcaactctt
    13561 tgcataagca gagttggcag gcgatttatt cctgagacca aaacaggatt attgatacaa
    13621 cttctaatta cagttttgtg aattggatct gcagagagtg caaggcttct catcgctctt
    13681 gctagccctc ttagcgtcaa agcaaataac ggcgctccgc agccatcaac tgcaacatgt
    13741 gtaatctgct ccccacttaa tatttcaaac tcattagcaa tcgcaagctg cagcaggtgg
    13801 cttggttctt tatagcttgc taaatcccaa ttatttgtct tgcatgttgc aaccatcgct
    13861 gcatgttttc cgctgcaatt agcagcaaga gatgatggcg atcccaccca agcagctctt
    13921 tcaaatgcgc caagtggttt atcaggagta ttttgaagtg ctgactcatc aaggccaacg
    13981 cttgcaagag ttgatctaac tacctcaagg tgagcatcac ttcctgcatg acttgcgcag
    14041 gcaagtgcta tctgctgctc atttagcttt aaacctgctc ttagcatcgc agaagcctgc
    14101 agagatttaa tcgctgagcg gggatagata agttgatcca actcaccaag tgctaatact
    14161 tccctgccat cgctatcaag aattaaaaga tggccactat gaattgattc cactaaatca
    14221 tttcttacaa cttcaagtag aacctcacct gagttcatag cttttaagaa attcttggaa
    14281 gagttgacca gatatgagct tgtaatttat ggccattaaa tgccatgtca taggcataga
    14341 agagctcgcc atcaacaagg ccatagagtc tctgcccgcc ttcaatagct ttagcagagg
    14401 gggccgaata tgcatgatcc atagctaatt gaatctttgg accatcaaat cttccaaccc
    14461 atccttcagc taatccggtg ctatgagaga ttaaaacttc caattgatta tctggcttaa
    14521 ctcgccaaaa tcccgtctct gatgaagcgg tttgaacaat ctcattattt tcatcaatga
    14581 tccaggagcg agaaaagtag gtaagaaagt ttcgtccatc atgagcaaag gtcacctctt
    14641 gagcaaattg aaaactctca attcctggat actcgccatg acctttaccg ctccagctgc
    14701 caactagcca ggcaagtgga tttagatcag ggtgaagtcc ttctggtatt tgaaatgcca
    14761 tttagccatt cttccttaat tttctccagc ctttagcagc agcgccaaga agaaataacg
    14821 ataggagtag tgaggtaaga atcaaggcaa tagaagttat tagctccatc ttcttaacct
    14881 atctgcctat tcaatcgcca ccaatatctc ttggct
//
LOCUS       DAOROD010000024        14823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_183_length_14823_cov_29.373510, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000024 DAOROD010000000
VERSION     DAOROD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_14823_cov_29.373510"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..329
                     /locus_tag="PLW53_05160"
     CDS             27..329
                     /locus_tag="PLW53_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HRD36842.1"
                     /translation="MENQRPEIYLSGGEPIEFTYKGQRYRIYALLSSWRESGGWWNRA
                     SDGLFRPDDGGRILWRVEAAPIGVMKTFEIERIEVIPEGASSAPGLWQIRPTSRPA"
     gene            326..3502
                     /locus_tag="PLW53_05165"
     CDS             326..3502
                     /locus_tag="PLW53_05165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRD36843.1"
                     /translation="MSFTHLHLASGFSFKYGTTLPHLLVEKARDFGMSSLALTDRDGL
                     YGAIRFTQSCVENGITPIIGVDITCKNQSRITLLATPGKGWRSLVRLVSELKSYGECD
                     LDFLRKHSNYTSNLILLQGPDSLIARAITNRQYEKALSLYNDSRELFQSQVIEIVSHL
                     IYGDGPRSTTHASRMLGFAEDNDLPAILSNAARMRLRQDAPIADILDASRRLLPLHKN
                     VVERENSEAFLKSSQEMHHLAEIITRASGQRNSRKLLSTTQDLAKQAELSPSQDIGLG
                     SIHLPEPELLGAKNQEELTIQLRQRSIAGINWRYGQSNSEVNQRLEKELGIIKSLGFE
                     SYFLTVADITDRARASGIRVAARGSGAGSLICHLLGISGVEPLSLGLLMERFCSPLRR
                     ALPDIDIDVESARRLEIYDQVFANYPMRSATVAMFDTYRARHAIRDVGAALSLAPMEI
                     DLVAKSLPHIRARNISQALINLPELANLNLTSPTWKMAVGLAERLDGLPRNVAMHPCA
                     VVISSAELYQRAPIEINASNYPMVQWDKDDVEAIGLLKLDILGVRMQSSLAYAISEIE
                     RSTGKVIDIDAIALDDSETFSLIQSAQTLGIFQIESPGQRELVGKLTPETFTDLIIDI
                     SLFRPGPVKSDMITPFLNARQGFKTREIFHPDLAEILSDTQGVVVFHEQVIEIIATLT
                     GCSLGQADEKRRALGSREGQQEVCDWFFAQAISRGYELKVITQIWDVLRAFASFGFCK
                     AHAAAFALPTYQSAWLKRHYPAAFLAGVLTHEPGMYPKRLLLDEARRNGLTIAPLDIN
                     HSDISYRVEGDNQRSIRLSLSDISGISSAEISAIIANRPYLDLADFVHRSSVSRPTAE
                     ALLMVGAFDQLYGAEINRRDLLLHLTDLYKWSGGSRTKVVTGSQLSLGFTPTLSKTGL
                     PDLEDHELVRNEVKYLGMDISHHMIEFYADFLNQIGAIKSNQLLEQRSGSLVLVAGVK
                     VALQTPPVRSGKRVIFLTLDDGYGCSDATFFEDAQHSYAEVLYNSQLLLIRGATRRTG
                     LRGISLRAIGAWDLSAAYQKWRSLAICE"
     gene            3495..4697
                     /gene="dinB"
                     /locus_tag="PLW53_05170"
     CDS             3495..4697
                     /gene="dinB"
                     /locus_tag="PLW53_05170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRD36844.1"
                     /translation="MSSQMGEHSTILHVDMDAFFASVSELDYPQYKGKPLVVGAGARG
                     VVLSANYAARKFGIKAAMPVSRAQRMAPNAIFIPPDHERYSQVSRKVMEIFFEFTPHV
                     EPLSLDEAFLDVTGSRKLFGSGRNIAELIRKRVFASEKITCSVGIATTKFIAKLASGR
                     CKPNGILEIAHDRVLTFLHPLPVSEIWGVGPKTNEELQALGLRTVADIANTPLETLKR
                     ALGPSAGVSLYELAWARDYREVVADAPEKSISAAETFAYDLEDQDEIFRELLRLTERA
                     GARLRARSLRSKTIGLKVRFSDFSNLTRSKTLPLSISGSHEIYEVVKDLYLGLKIAGS
                     RIRLLGVSLEQLVDQSGAVEQLELGERELGWSQAQVAIDRAIARFGSGSVKPARLVGE
                     GDDESEDS"
     gene            4754..5119
                     /locus_tag="PLW53_05175"
     CDS             4754..5119
                     /locus_tag="PLW53_05175"
                     /inference="COORDINATES: protein motif:HMM:NF022684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HRD36845.1"
                     /translation="MPLSEHEKRLLAEMEEALAADDPRLISTFSGKSPSRSRVVIGFA
                     LILLGIFILFGGLISQVPLIGVAGFIASLVGAVLAISNLGGLMNGVISKAQSRGPKRG
                     PGKWSQGFEQRWDRRNLGE"
     gene            5312..5743
                     /gene="mraZ"
                     /locus_tag="PLW53_05180"
     CDS             5312..5743
                     /gene="mraZ"
                     /locus_tag="PLW53_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRD36846.1"
                     /translation="MFLGTHEPRLDEKGRLILPAKFRDELASGLVITKGQERCLFVFP
                     SAEFARITEQLKQAPLTAKAARDYMRVMFAGAHDEIPDKQGRVMIPSGLREYADLEKD
                     CVVIGANTRVEIWDSKAWKSYLKDREKGFADVSEEVFPGLF"
     gene            5920..6867
                     /gene="rsmH"
                     /locus_tag="PLW53_05185"
     CDS             5920..6867
                     /gene="rsmH"
                     /locus_tag="PLW53_05185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRD36847.1"
                     /translation="MKLEHTSVLLNRCITVLSTSLENKEKPVLVDATLGLGGHSLALL
                     EKFPNLQIIGIDRDKSAIDKASQRLSSHLDRITIFHAVYDQTPKLLDSLGISSVDGIL
                     FDLGTSSMQLDQSERGFSYAQSAPLDMRMDQSSGITAQEIIDSYSRGELIRIIRSYGE
                     EKFAQRIVDNIIEARASGLIKSTSDLADLIKRSIPAPARRLGGNPAKRTFQALRIEVN
                     QELAILERAIPACLERLNVGGRMVVMSFQSLEDKIVKNFFTQATESKTPLGLPIEIKA
                     LSAKFSLVFNGSQKASDAEILDNSRAKSVRLRAIERVAA"
     gene            6867..7289
                     /locus_tag="PLW53_05190"
     CDS             6867..7289
                     /locus_tag="PLW53_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36848.1"
                     /translation="MALSVLSPAFERSKKLAIAKSSKVILKLVPELRPTPGQQATDRL
                     FYSIVGATLALGLLGLLGINILLGNDAVRVRELKIEAIMVNEEREEALRQVALLSTSE
                     SLAERAISLGMIPSSSPTFLDISEPIPLEVEKAAKDKP"
     gene            7286..9133
                     /locus_tag="PLW53_05195"
     CDS             7286..9133
                     /locus_tag="PLW53_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD36849.1"
                     /translation="MSHHKLVEKRISYLVAISLVILIAFAVRLIDIQGVRAAGLANKA
                     ENELTKKSVIMAPRGAITDINGTEFARSVSAYRILVDQAIVDHPKELAQIAAPILDVD
                     QSWLKDQLIGERRYVVISQAVKPEVWRKLEAAVDAYNEEVAKSENGLAKRLMGFFAER
                     IYIREYPEGELAAAVIGFINRAGVGAAGLEYSMNSTLSGVDGLYTYANAAGTIIPGTQ
                     KITAEAVRGNTVRLTIDRDVQWVAQEAIRTVVKSSRALSGTVVVMDPKSGQVLAHATY
                     PSFTPGDTKGVDPYRWQNPSVQEVYEPGSTGKVMTIAAAVEEGKISPETVMTIPWKLK
                     RSNKTFSDHEKHPTQKLTLTGALAVSSNTGAIKVGEMLSNDTLHSYLKKFGIAEKANS
                     GLPGEETGSLRDVKDWSGTTAPTVAFGQGYSLTALQATSVFATIANDGLRVTPTVIAG
                     ISDGAGRYTPASQQKSVRVISPDTAKKMRLMMESVATNRGTAASAAIEGYRVAGKTGT
                     AQRYNPKCGCYSGYTASFIGFAPADKPEYVVSVTIQDPKGLHWGGYLGGPVFKKVMSF
                     VLKDKAIRPTAPATITYALNAKELKAKIELMTGGAQASGAKRDKASENG"
     gene            9126..10631
                     /locus_tag="PLW53_05200"
     CDS             9126..10631
                     /locus_tag="PLW53_05200"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRD36850.1"
                     /translation="MAEMHRLEANIKKLGDVASFLGVKVSEEFESLEVTGLSSSSNRV
                     KNGELFIALPGEKHHGARFAKDAKDSGAIAILTDSEGLDLASKSLGNFPILVVENPRS
                     RIGYLADWFHNFPSRALFLAGITGTNGKTTTSYLLKQIWEFAGVKAGLIGTIGIEIGE
                     ERFSASHTTPEADQLHYILSIMQERKVSAAAIEVSSHALSLNRVDGAYFSAAAFTNLT
                     QDHLDFHGDMENYYQAKKKLFDSMRSKVSFINIDDSYGVRLAEEISMPSKTISRSNNK
                     ASWFYQDIKSTAKGYRVEVRGTGGILISGELNLIGEHNLDNALLAMAIAFESGVDPLV
                     IGDSLATLKAAPGRLESIDCGQNFLALVDYAHTPDAVEKVLASLRANTDSKVIAILGC
                     GGNRDKSKRPLMGKALLSGSDIAIFTSDNPRDEDPEQILKEMTGSLKLSTPSAVISDR
                     QSAIKYGVERAEAGDILVILGKGHETGQEIKGVKHAFSDQEVLQKCIEAKL"
     gene            10628..12013
                     /gene="murF"
                     /locus_tag="PLW53_05205"
     CDS             10628..12013
                     /gene="murF"
                     /locus_tag="PLW53_05205"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRD36851.1"
                     /translation="MIEMKIEQMAQIMEGSAHGQVEITSSTAFQFDSRQINSGDIFLA
                     LKGEKADGHDYVSDANARGASLSIVNRPVPTPHILVGDVLLALAKLAAYVRRELKDLK
                     VIGITGSQGKTTTKDLLLSILKAEGETVASIGSFNNELGVPLTLLRCTKESKYCILEM
                     GARNSGDIASLTAIASPDVGAVLKVGNAHIGQFGSQEMIAKTKGELIAGLQSGAVAVL
                     GTYDSFTPKMESAQGVKRVTFGDSPKCDVRAADIEARGGFAHFDLVIGSERQAVGLQI
                     LGLHQISNALAAAAIANALDIGVSRIATALSNHQSISKWRMELSEANGLTLINDSYNA
                     NPESMEAALNVLALLTQEKGGRSWAFLGQMHELGDNSDQMHEEIGKKVADLGIDYLVA
                     IKSKAYLSKIDSSLTSARYLSSWQESLDLFREFNPGDVILVKASRAEGLDELASAIKA
                     KAMSEQEGESK"
     gene            12010..13068
                     /gene="mraY"
                     /locus_tag="PLW53_05210"
     CDS             12010..13068
                     /gene="mraY"
                     /locus_tag="PLW53_05210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRD36852.1"
                     /translation="MRGILISGATAFLLSFLLTPVFIKFLSRKGYGQQIRDDGPKTHH
                     TKRGTPTMGGVILILAAIAGYLISHLFTGAALTTSAVLVIGLIVSLGLIGYLDDWLKV
                     VKQRSLGLKAREKLLTQGIVAGAFGYLATRFPDDLGLTPMSQYLSTVKDTSIKLGIVL
                     LILLAILMVLGASNGVNLTDGLDGLATGAAVMSFLAFVLIGVWEFGQRCEVVGSGNCY
                     NVRDPLDLAVLAAALAGSCAGFLWWNASPARIFMGDTGSLALGGALAGLAMTLRTELL
                     LIPIGGLFVIITLSVIIQTTYFKISGGKRVFKMAPLQHHFELLGWGEVTIVIRFWIIA
                     GLCVAAGLGLFYAQWVVA"
     gene            13065..14432
                     /gene="murD"
                     /locus_tag="PLW53_05215"
     CDS             13065..14432
                     /gene="murD"
                     /locus_tag="PLW53_05215"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRD36853.1"
                     /translation="MNQSFIDSLRGAKCILFGAGVTGAPTLEFLKSNGASVISVDEKV
                     QENGIKRTLDTDDLVGVSFAVVSPGWRVDHPLISLVRGAGIELISEIDLAWKVKEQIC
                     PNQRWIALTGTNGKTTTIQMLESMLVESGINAKACGNVGYTAIEAVVKSNADILALEL
                     SSFQLEWSNLAHFESAAILNIAEDHIDWHQSFENYLSAKLKISELADLVILNKDDQHL
                     AKSGKNISKPITWISLQVPSKGEIGLVENIIVDRAFIKGDAEALFELGDVTPSVGHNV
                     SNAMAAAALARSVGAKPDAIAQGLRNFKLDHHRLEVVHKNSGITFINDSKATNPHAAI
                     AALYSQMESIWIAGGLAKGAAMDELAQRCKSRIKAAILIGSDAPIIKAALLKQSPNLA
                     IYTTDLSLKGIDVMHSVMSIVKEIAVSGDVVLLAPACASMDQFKNYADRGESFTLAAR
                     QAFNE"
     gene            14425..>14823
                     /locus_tag="PLW53_05220"
     CDS             14425..>14823
                     /locus_tag="PLW53_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRD36854.1"
                     /translation="MNKSKPLFATPASAYYLILGSVIALSSLGLVMVLSASSVKSLRE
                     SGNSFSFVLRQALFLISAAGLAWVAMKLRYELWKPIAQVSLVLSCALLVLPQIGGIGK
                     TVGGNTNWIGIGSFTLQPSEFAKLGLILWCA"
BASE COUNT         4218 a         2995 c         3547 g         4063 t
ORIGIN      
        1 tagtattcga acaaatgttc gaaaagatgg agaatcagag accggagatc tacctatccg
       61 gcggtgagcc aattgagttc acctataaag gtcagcgcta tcgaatttat gccctgctta
      121 gctcttggcg tgaatctggt ggttggtgga atagagctag cgatggctta tttcgcccag
      181 atgatggcgg acggatttta tggcgcgtag aggcagctcc tattggagtg atgaaaactt
      241 ttgaaataga acgcattgaa gtaattccag aaggggccag tagcgctcct ggcctttggc
      301 agattcgccc cacatctaga ccagcatgag ttttactcac cttcatcttg caagtggatt
      361 ttcatttaaa tatggaacca ctcttcctca tcttctagtt gagaaggctc gcgactttgg
      421 catgagctca cttgcactaa ctgatcgcga tggcttatat ggcgcaattc gatttactca
      481 gagctgtgtg gaaaatggca ttactccaat tattggagtt gatatcactt gcaagaatca
      541 gagtcgaatc acattacttg ctaccccagg taaaggctgg agatctttag taaggctagt
      601 gagcgaactt aaaagctatg gtgagtgtga tttagacttt ttaaggaaac actctaacta
      661 caccagtaac ttaatattac tacaaggccc agactcttta attgctagag ctattaccaa
      721 tcgtcaatat gaaaaagcgc tatcgctata taacgatagc cgcgaactat ttcaaagcca
      781 agtaattgaa attgtctctc atctaattta tggagatgga ccaagatcaa ctacccatgc
      841 ttcaaggatg cttggttttg ctgaagataa tgatcttcca gcgatactta gtaatgctgc
      901 cagaatgcgc ttaaggcagg atgcgcctat agcagatatt ctagatgcaa gtcgaagact
      961 cttgccgctt cataaaaatg ttgtagagcg cgaaaatagc gaggcctttc ttaaaagctc
     1021 tcaagagatg catcacttag cagagataat tactcgcgct tcggggcaga gaaatagccg
     1081 taaattatta agtactaccc aagaccttgc taagcaggca gagctatcgc ctagtcaaga
     1141 tattggcctg ggatctatcc atcttccaga gcctgagctt ttaggggcta aaaatcaaga
     1201 agagctaact attcaactaa ggcagcgctc aattgcgggt attaattggc gctatggcca
     1261 gagcaatagt gaggtaaacc aaagactaga aaaagagtta gggattatta agagtctagg
     1321 ttttgaatca tattttctaa ctgttgccga tattacagat cgcgcgcgag cctctggaat
     1381 tagagtagcg gcaaggggaa gtggggctgg atcattaatt tgtcatctct taggaatttc
     1441 tggagttgaa ccactctcac taggtttatt gatggagcgc ttctgctctc cacttcgaag
     1501 agctctacct gatattgata ttgatgtgga atctgctaga aggttagaaa tttatgatca
     1561 ggtatttgca aattacccaa tgcgatctgc aacagttgca atgtttgata cctatagagc
     1621 tcgccatgcc attagagatg tgggggcagc tctatcactt gctcctatgg agatagatct
     1681 agttgctaaa tcactgccgc atattagagc tcgcaatatt tcacaggcgc taattaatct
     1741 tcctgaactt gctaatttaa atctaactag cccgacctgg aaaatggcag ttggccttgc
     1801 agagcgctta gatggcctac ctagaaatgt tgcgatgcat ccctgcgcgg tggtgatctc
     1861 cagcgctgag ctatatcaga gagctccaat tgagataaat gctagtaatt atccgatggt
     1921 gcagtgggat aaagatgatg tggaagctat tgggctttta aagctagata ttttaggagt
     1981 tcgaatgcaa tcatctctgg cctatgccat atctgagata gagcgaagta ctggaaaagt
     2041 aatagatatt gatgcaatag ctttagatga tagtgaaacg ttttcgctca tccaatctgc
     2101 tcagacttta ggtatctttc aaatagagag cccaggccag cgtgaattag ttggaaagtt
     2161 aacccccgag acttttactg atttaataat tgatatctca ctttttagac cagggccagt
     2221 aaagagcgac atgatcactc catttctaaa tgcccgccaa ggttttaaaa ctagggagat
     2281 attccatcct gatttagctg aaatcttgag tgatacccaa ggggtagtgg tatttcacga
     2341 gcaagttatt gaaattatcg ccaccctaac tggatgctct cttggccaag ccgatgagaa
     2401 gcgaagggct cttggcagta gagaggggca gcaggaggtc tgtgattggt tctttgctca
     2461 agctatatca agaggctatg agttaaaagt tattactcaa atctgggatg tcttaagagc
     2521 ctttgcctcc tttggattct gcaaagccca tgccgctgcc tttgcacttc ccacttatca
     2581 atctgcttgg ttaaagcgcc attacccagc agcatttctt gccggagttt taactcatga
     2641 acctggaatg tatcccaagc gcctgctatt agatgaggct agacgaaatg gattaactat
     2701 cgctcctctt gatattaatc actctgatat tagctatcga gttgaaggtg ataatcagag
     2761 aagtatcagg ctatctctta gcgacatctc aggaattagt agcgcagaga tatcagcaat
     2821 tattgctaat cgcccttatt tagatctagc tgactttgtt catcgcagct cagttagcag
     2881 gccaactgct gaagccttat taatggtggg ggcctttgat cagctctatg gcgctgagat
     2941 aaatcgtaga gatttattac tacatctaac tgatttatat aaatggtctg gtggtagtag
     3001 aactaaagta gttactggtt cacagttaag cctaggtttt acccccactt tatctaagac
     3061 agggctacct gatttagaag atcatgaact ggtaagaaat gaagttaagt atctagggat
     3121 ggatatctct catcatatga ttgaatttta tgctgatttt ctcaatcaga taggagcaat
     3181 aaaatctaac cagcttctag aacaaagatc aggatcttta gtattggtag ctggggttaa
     3241 agtagcgctg caaaccccac cagttcgctc tggcaagaga gttatctttc taactcttga
     3301 tgatggatat ggctgtagcg atgcaacatt ttttgaagat gctcaacata gctatgccga
     3361 ggtgctctat aactcgcagc tactattaat tcgcggcgct acccgtagaa ctgggctacg
     3421 cggtatctca cttagagcta ttggggcatg ggatttatca gctgcttatc aaaagtggcg
     3481 tagcctagct atctgtgagt agccagatgg gcgagcattc caccattctc catgttgata
     3541 tggatgcatt ttttgcctca gtttctgagc tggattatcc gcaatataaa ggaaagccgt
     3601 tagtagttgg cgcaggggct aggggagtag ttctttcggc aaattatgct gctagaaaat
     3661 ttggaattaa agctgcgatg ccagtatcta gagcgcagcg gatggcgcca aatgcaatat
     3721 ttattccgcc agatcatgag agatattcac aagtttcaag aaaagttatg gagattttct
     3781 ttgagttcac tcctcatgtt gagccgctct ccctagatga ggcttttctt gatgtaactg
     3841 gatcaagaaa actatttggc agtggtcgaa atattgcaga gttgataagg aagagagttt
     3901 ttgcatcaga gaaaataacc tgctcagttg gaattgctac tactaaattc atcgccaaat
     3961 tagcatccgg gcgctgtaag ccaaatggaa tacttgaaat agcccacgac cgagtcttga
     4021 cttttcttca tccacttccc gtttcagaaa tctggggagt ggggccaaaa acgaatgaag
     4081 agctgcaagc tctaggcctt cgaaccgttg cagatattgc caatactcct cttgaaactt
     4141 taaagcgagc acttggtcca agtgctgggg tatcacttta tgaacttgct tgggctaggg
     4201 attatcgcga agtagtagcc gatgctccag agaagagcat tagcgcggca gaaacttttg
     4261 catatgatct cgaggatcaa gatgagatat ttagagaact cttgcgatta actgaaagag
     4321 ctggcgcaag gcttagggct cgcagtttgc gcagtaaaac cattggttta aaggtacgtt
     4381 tctctgattt ttctaatctc acccgatcta aaactctgcc actatcaata agtggcagcc
     4441 atgaaatcta tgaagtcgtt aaggatctct atctagggct caaaattgct ggctccagaa
     4501 tccgacttct aggggtttca cttgagcagt tagttgatca aagtggcgca gttgagcagt
     4561 tagagctagg agagcgggag cttggatgga gccaggcgca ggttgctatt gatcgggcca
     4621 ttgctcgctt tggaagcggc tctgtaaaac ctgctcggct agttggagag ggcgatgatg
     4681 agagcgaaga ttcatagcaa cttctcactc tttcgacttt ccagatagcc ccacctctac
     4741 tattgtcagt cctatgccac tatctgagca tgaaaaacgg ttattagccg agatggaaga
     4801 ggcgcttgcc gccgatgacc ctcgcctgat ttcaactttt agcggcaaga gcccatctag
     4861 gagccgggtg gtaattggtt ttgccctaat ccttctaggc attttcattc tcttcggcgg
     4921 cttgatttct caagttccac ttattggggt tgctggattt atcgcctcac ttgtgggggc
     4981 agttcttgct atctcaaatc ttgggggact gatgaatggg gttatctcca aagcccagag
     5041 cagagggcct aagagagggc caggcaagtg gagccaaggc tttgagcaga gatgggatcg
     5101 ccgaaatctc ggtgaatagt tagttagatc agcacaagtg cccgcctctg gcgggttttt
     5161 ttatgcccaa aaccggagag tggaggagag cttttccgag cctgctcccg tgtggagata
     5221 ggtggttgaa aatggaggaa aagggagtaa agtggggaat taagcagaac gggggttctg
     5281 cgcaataagg caagtagggg aggtctgtta agtgtttctt ggcacccatg aacctcgctt
     5341 agatgaaaaa gggcggttga ttcttcccgc caaattccgt gatgaattgg cctcaggttt
     5401 agtaattact aaaggacaag agcgttgttt atttgttttt ccttctgctg aatttgcacg
     5461 aattactgag cagctaaaac aagcgccact tactgcaaaa gcagctcgcg attacatgcg
     5521 tgtgatgttt gccggtgcac atgatgaaat tccagataag caaggtcgcg taatgattcc
     5581 ttccggctta agagagtatg cagatttaga aaaagattgc gtagtaattg gtgcaaatac
     5641 tcgagttgaa atttgggatt ccaaagcatg gaagagctat ctaaaagatc gtgaaaaagg
     5701 ctttgctgat gtttctgagg aggtatttcc aggcctcttc taacaagggg caaaaaaaca
     5761 aaaatctcct ctctcctctg gccaccgccg accttcaaat aaacggcgcc ccttccccgg
     5821 tgccgtttat cccccgcaat ccgtcggccg gcggtgacca gagtagagag taagaaagaa
     5881 gaagaagtta acaactacta ataattaagg agtgtaggga tgaagctaga acacacatca
     5941 gttctgctta atcgctgcat cactgtttta tcaacctcac tagaaaataa agagaagcca
     6001 gttttagttg atgccacttt gggtttaggt gggcactcac tagcgctatt agagaagttt
     6061 ccaaatttac agataattgg tattgataga gataaaagcg caattgataa agccagtcaa
     6121 cgtctatctt ctcatttaga tcgcatcaca atttttcatg ctgtttatga ccagacccca
     6181 aaactccttg attctcttgg aatttcatcg gttgatggca ttctctttga tttaggcact
     6241 tcgtcaatgc agttggatca atctgagcgt ggcttctcct acgctcagag cgctccactt
     6301 gatatgcgca tggatcaaag cagtggaatc accgcccaag aaattatcga tagctactcc
     6361 aggggcgagt taattcgcat tattcgcagc tatggcgaag agaaatttgc tcaaagaatt
     6421 gtagataaca tcattgaggc aagagcaagt ggtttaataa aaagcacaag cgatcttgca
     6481 gacttaatta agcgatcaat tccagctcca gcaagacgtc ttgggggaaa tcctgctaag
     6541 cggactttcc aagcacttcg catcgaagtt aatcaagagc tagcaatctt ggagcgagca
     6601 attcctgcct gccttgagcg cttaaatgtt ggtggtcgga tggttgtgat gtcattccaa
     6661 tcgctagaag acaagattgt taaaaacttt ttcacgcaag caacagagtc aaagactcca
     6721 cttggtcttc caattgaaat taaggctcta tctgcaaagt tctctttggt atttaacgga
     6781 agtcagaagg ccagtgatgc tgagattcta gataactcgc gggcaaagtc ggtaagactt
     6841 agagcaattg agcgggtggc tgcctaatgg ctctatcagt tcttagccca gcttttgagc
     6901 gttccaagaa attagcaatt gcgaagtcat caaaggtaat ccttaaactt gtcccagagc
     6961 ttcgcccaac accaggtcaa caagcaactg atcgcctctt ttattcaata gttggcgcca
     7021 cattagccct tggcctactt ggacttttgg gcataaatat tcttctagga aatgatgcag
     7081 taagagttag agagctcaag atcgaagcaa tcatggttaa tgaagagcgg gaagaggctc
     7141 ttagacaggt ggccctgctt tcaacttcag aatctctggc agagcgagca atatcacttg
     7201 gaatgattcc aagttcttca ccgacatttc ttgatatttc agagccaatt ccgcttgagg
     7261 tagaaaaggc ggctaaagat aaaccatgag ccaccataag ttagttgaga aaaggattag
     7321 ctatctagtc gctatttcgc tagtaattct catagctttt gcagttcgat taattgatat
     7381 ccaaggagtt agggcagcgg gcctggcaaa taaagctgaa aatgaactaa ctaagaaatc
     7441 agtgattatg gctccaaggg gtgcaatcac agatatcaat ggcaccgagt ttgctagaag
     7501 tgttagcgcc tatcgaatcc tggttgacca agcaatcgtg gatcatccaa aggagctagc
     7561 ccaaattgca gcaccaattt tagatgttga tcaaagctgg cttaaggatc aattaattgg
     7621 cgagaggcgt tatgtagtaa tcagccaggc agttaaacct gaagtttgga gaaaacttga
     7681 agcagctgta gatgcctaca atgaagaagt tgctaaaagt gaaaatggcc ttgctaaaag
     7741 attaatgggc ttctttgccg agcgaatcta tattcgcgaa taccctgaag gtgaattggc
     7801 agctgcagta ataggcttta tcaatcgcgc aggagttgga gctgcaggcc ttgaatacag
     7861 tatgaattcc actctaagcg gagttgatgg gctctacacc tatgcaaatg cagcaggaac
     7921 aataattcct ggaacgcaga agataactgc agaggcagtt cgtggaaata ccgttagatt
     7981 gacgattgat agagatgtgc aatgggtagc acaagaggca attagaacgg tagtaaagag
     8041 ctctcgggcg ctctctggaa ctgttgttgt tatggatcca aaatctggtc aagttttagc
     8101 tcatgctact tatccctcat ttactccagg ggataccaaa ggagtagatc cttatcgctg
     8161 gcagaaccct tcagttcaag aagtctacga accaggctct actggcaaag tcatgaccat
     8221 tgctgctgca gttgaagagg gaaagatttc acctgaaacg gttatgacta ttccttggaa
     8281 attgaagcgc tcaaataaaa cctttagtga tcatgaaaaa caccccacac agaaattaac
     8341 tcttactggc gcccttgccg tatcaagtaa tactggcgcc ataaaagtgg gggagatgct
     8401 ctctaatgac acgcttcact cttatctaaa gaagtttggc attgccgaga aagccaactc
     8461 aggacttcct ggcgaggaga ctggaagttt gcgtgatgtt aaagattggt caggaacaac
     8521 tgcgccaaca gtggcatttg gacagggtta ctcactcaca gctcttcaag caacatcggt
     8581 atttgcaact attgcaaatg atggccttcg tgtaacgcca actgttatcg caggaattag
     8641 cgatggagca gggcgctaca ccccagcaag tcaacaaaag tcagtaagag ttattagtcc
     8701 agatactgca aagaagatgc gcctaatgat ggagagcgtt gcaactaatc gaggaactgc
     8761 tgcttcagct gctattgaag gctaccgagt ggcaggaaaa actggaactg cccagagata
     8821 taacccaaag tgtggttgct atagcggata caccgcatcc tttattggtt ttgcgccagc
     8881 tgataagccc gagtatgtgg tttcagtaac gattcaagat ccaaaaggcc ttcattgggg
     8941 cggctatcta ggtggaccgg tatttaagaa agttatgagc tttgtgctaa aagataaagc
     9001 gattagacca acggcgccgg caacaattac ctatgctcta aatgcaaaag agttaaaggc
     9061 aaagattgaa ttaatgactg gcggcgctca agcatcaggg gcaaagcgag ataaagcgag
     9121 tgaaaatggc tgagatgcat cgcttggagg ctaatataaa aaagctcggc gatgtcgctt
     9181 catttctagg cgtgaaagtt agtgaagaat ttgagtcact tgaagtaact ggtctttcaa
     9241 gttcatcaaa tagagttaaa aacggagaat tattcatagc gctaccaggc gaaaaacacc
     9301 atggagcacg atttgcaaaa gatgccaaag actccggcgc aattgccata ctcaccgata
     9361 gtgagggctt agatcttgcc tccaaatcac tcggcaattt tccaattctt gtcgtagaaa
     9421 acccaagaag cagaattggt tatttagctg actggttcca taactttcca agcagagctt
     9481 tatttcttgc aggaatcacc ggcactaacg gcaagacgac aacctcatat ctattaaaac
     9541 agatttggga gtttgctggg gtgaaagctg gactcattgg aaccattgga attgaaatag
     9601 gagaggagcg atttagcgcc agccatacca ctcctgaagc tgatcaactt cattacattc
     9661 tctcgattat gcaagagaga aaggttagcg cagctgcgat tgaagtaagt agccatgcgc
     9721 tttcgcttaa tagagtagat ggtgcctatt tctctgctgc tgcctttact aatttgaccc
     9781 aagatcatct agattttcat ggagatatgg aaaattacta ccaagcaaag aagaagttat
     9841 ttgactccat gcgcagcaag gtctcattta tcaacattga tgactcctat ggagttcgcc
     9901 tcgcagaaga gatttcaatg ccatcaaaaa ccatctctcg aagtaacaat aaagctagtt
     9961 ggttctacca agatataaag agcaccgcaa agggctatcg tgtggaagtt agaggcaccg
    10021 gcggcatatt gatatcaggt gagttaaacc taattggcga gcacaactta gataatgctc
    10081 ttttggctat ggcgattgct tttgaaagtg gtgtagatcc gcttgtgatt ggagactctc
    10141 tagcaactct taaggctgct ccaggtcgcc tagaaagtat tgattgtggt caaaacttcc
    10201 tagctctagt tgattacgct cacactccag atgcagtgga aaaagtcctt gcatcactta
    10261 gagcaaatac tgactcgaag gtaattgcaa tccttggatg cggcggtaat cgtgataaat
    10321 caaaacgccc tctaatggga aaggcgctac tttctggaag tgatatcgcc atattcacta
    10381 gcgataatcc aagagatgaa gatcctgagc aaatcctaaa ggagatgacc ggatcactaa
    10441 agctctctac tccaagtgct gtaatcagtg atcgccaatc agcaattaaa tatggagtag
    10501 agagagctga agctggcgat attttagtaa ttctgggcaa gggccacgaa actggtcaag
    10561 aaataaaggg cgtcaaacat gcctttagtg accaagaagt tttacaaaag tgcatcgagg
    10621 ccaaattatg attgaaatga agatagagca gatggctcag attatggagg gcagcgccca
    10681 cggccaagtt gaaattacaa gttcaacagc atttcaattc gattcaaggc agattaattc
    10741 aggagatatc ttccttgcac taaagggcga gaaagccgat ggtcatgatt acgtatctga
    10801 tgcaaacgca aggggagcat cgctaagtat cgttaatcgc ccggttccaa ctccccacat
    10861 tttggtaggt gatgtattac ttgctttagc taaattagca gcctatgtaa gaagagaatt
    10921 gaaagaccta aaagttattg gaataacggg aagccaagga aagacaacaa ctaaggactt
    10981 attgctctcg attcttaagg ctgaaggaga aactgttgcc tcaattgggt ctttcaataa
    11041 tgaactggga gtccccttga ccctgcttcg ttgcacgaaa gagagtaaat actgcatatt
    11101 ggaaatggga gctcgaaata gtggcgacat tgcctccctc actgcaattg caagccctga
    11161 tgttggcgct gtgttgaagg tgggcaatgc tcacattggt caatttggtt cacaggaaat
    11221 gattgctaaa actaagggcg agttaattgc tggtctacaa agtggcgctg ttgctgtttt
    11281 gggaacttat gactccttta ctccaaagat ggagagcgct caaggggtaa agagagttac
    11341 cttcggagat agccccaaat gtgatgttcg tgctgcagat attgaagcta gaggtggatt
    11401 tgctcacttt gatttagtca ttggcagtga gcgccaggca gtgggattgc agattctagg
    11461 tttacatcag atctccaacg ccctagctgc agcagcaatt gctaacgcat tagatatcgg
    11521 tgtatctcgc attgcaactg ccctctccaa tcatcaatcg attagcaagt ggcgtatgga
    11581 actaagtgag gctaatggct taacgctgat taatgattca tataacgcaa atcctgaaag
    11641 tatggaggca gcacttaatg tgctcgctct actgacacaa gaaaaaggtg gacgctcttg
    11701 ggcattccta ggtcagatgc atgagctagg agataattca gatcaaatgc atgaggaaat
    11761 tggtaagaaa gttgcagatc ttggaatcga ttacttggtc gcaataaaga gtaaagccta
    11821 tttgagcaaa attgattctt cgttaactag cgcaaggtat ctttcttcct ggcaagaatc
    11881 cttagatctc tttagagagt ttaatccagg cgatgtaatt cttgttaagg catcaagagc
    11941 tgaaggcctt gacgaattag caagtgcaat taaggcaaag gctatgagtg agcaagaggg
    12001 agagagcaaa tgagaggtat tttaatttcc ggggctacgg cctttctgct tagcttcctt
    12061 ctaacgcctg tatttattaa gttcctatcg cgcaaaggct atggccaaca gattagagat
    12121 gatggtccaa agactcatca cacaaagcgt ggaacaccaa ccatgggtgg agtaattctg
    12181 attctggcgg caattgctgg ttacttgatt tcacatcttt ttacgggagc agctttaaca
    12241 acttctgcag tattggtaat tggtctaata gtaagcctcg gtttaattgg ctatcttgat
    12301 gattggctaa aggttgtaaa gcagcgctcg cttggcctta aagcaagaga aaagcttctt
    12361 acccaaggca tagttgctgg agcatttgga tatctagcaa ccagatttcc agatgatctt
    12421 gggcttactc ctatgtcgca atacctctca acggtgaaag acacttcgat aaaacttgga
    12481 attgtcctct taattcttct agcaatttta atggtacttg gcgcaagtaa tggcgtgaat
    12541 ttaactgatg gcctagatgg tttagcaacg ggtgcggcag tgatgtcatt tctcgccttt
    12601 gttctaattg gtgtttggga gtttgggcag cgctgcgagg tagtgggcag tggaaattgc
    12661 tacaacgtgc gcgatcctct agatcttgca gtactagccg cagctcttgc tggctcttgc
    12721 gcaggctttc tatggtggaa tgcatctccg gcaagaattt ttatgggaga taccggctct
    12781 cttgctctag gtggagcgtt agcgggcctt gcaatgacat tgagaactga actacttctc
    12841 attccaattg gcggactatt tgtcattatt actctttcag taattattca aactacttat
    12901 ttcaaaataa gtggaggcaa gagagtattt aaaatggcgc cgctccaaca ccattttgag
    12961 ttacttggtt ggggcgaagt cacaatcgtt attcgcttct ggattatcgc tggactttgc
    13021 gtagctgcag gactcggact cttctatgca caatgggtgg ttgcgtgaat caaagcttta
    13081 ttgattctct aagaggtgca aagtgcattc tctttggcgc gggcgttact ggagcgccaa
    13141 ctcttgaatt cctaaaatca aatggcgcat cagtgatttc agttgatgag aaggttcaag
    13201 aaaatggaat taagcgcacc ttagatactg acgatctggt aggagttagc tttgcagttg
    13261 tctctccagg ctggagagtt gatcatcctt taatctcgct ggttagagga gcaggtattg
    13321 aattaattag cgagattgac cttgcctgga aggtcaagga gcagatatgc cctaaccagc
    13381 gctggatagc gctaactggc accaacggta agacaacaac aattcaaatg ctggaatcaa
    13441 tgctcgttga atcaggaatt aatgcaaaag cctgtggaaa tgtgggatat accgcaattg
    13501 aggcggtagt taaatcaaat gccgatattt tggcactaga actttcatca tttcaattag
    13561 agtggagcaa tcttgctcac tttgaatcgg cagcaattct taacattgcc gaagaccata
    13621 tcgattggca tcaaagcttt gagaattatc tctccgctaa gttaaaaatc tcagaactgg
    13681 cagatttggt gattctcaat aaagatgatc agcatttggc aaagagcggt aaaaatattt
    13741 ctaagccaat tacctggatt tctcttcaag ttccgagtaa gggcgagatt gggctggttg
    13801 aaaatatcat tgtcgatcgc gcctttatca agggtgatgc tgaagcacta tttgagctag
    13861 gtgatgtaac cccctctgtt ggccataatg tttccaatgc gatggctgct gcggcgctgg
    13921 ccagaagtgt tggagcaaaa ccagatgcaa tagcccaggg gctacgaaac tttaaactag
    13981 atcatcatcg attagaggta gtacataaaa acagtggaat aactttcatt aatgactcaa
    14041 aggcaactaa tccccatgcc gccattgctg cgctgtattc tcaaatggag tcaatttgga
    14101 tagctggggg tcttgctaaa ggagcagcta tggatgaact ggctcaaaga tgcaagagcc
    14161 gtatcaaggc tgcaatattg attggaagtg atgccccaat cattaaagct gcgctattga
    14221 agcagtctcc gaatcttgca atctatacaa ctgatttaag cttgaaagga atcgatgtaa
    14281 tgcacagcgt tatgagtatt gttaaagaaa ttgctgttag tggtgatgtg gtgctactag
    14341 ctccagcatg tgcatcgatg gatcaattta agaactatgc cgatcgtggc gagagtttta
    14401 ctctggcagc aaggcaggca tttaatgaat aaatctaagc ctttatttgc aactcccgcc
    14461 tctgcttatt acttgatttt aggctcagtt atcgccctct cctcgctggg attggttatg
    14521 gtgctaagcg cctcatcagt aaagtcgttg cgagaatctg gcaactcttt tagctttgta
    14581 cttcgccaag cgcttttcct tatttctgca gcagggcttg catgggtagc gatgaaactt
    14641 cgctatgagc tttggaagcc gatagcccaa gtctcactag ttctttcctg cgcccttcta
    14701 gttcttcctc agattggtgg aattggcaaa actgttggtg gtaataccaa ctggattggt
    14761 atcggaagct ttactttgca gcccagtgag tttgcaaaat taggtttaat cctctggtgc
    14821 gct
//
LOCUS       DAOROD010000025        13583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_216_length_13583_cov_24.052779, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000025 DAOROD010000000
VERSION     DAOROD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216_length_13583_cov_24.052779"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="PLW53_05225"
     CDS             <1..1868
                     /locus_tag="PLW53_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD36855.1"
                     /translation="FLSLGEEMKLNRIAKVAIATAAAAGLAVSTLTPAGAATRSTVVL
                     VTSNQMTSLNSGTPDTNLTVNSEIGYLQGFGFNYYDNAPKLVRNTIFGSYKIVSQNPF
                     KVQYTVNKGKVWSDGTPITGVDLLLSHVLASSSYSFTAKLGDPTDPEVRPAFNALGYG
                     GIYDQTHVGLPTLSADKMSVTIEYDSKIPDWEITGPGPAAVHALIHLAEGKTELQSAA
                     ANLAAKAKFEKAFYNYDTKLMKAAGKVWSEAYNITDINDSTNPLLLIGNGGYKIKSAV
                     TGQSVTLVLNPKHVTNPSGPKTNGVKTIIFRVVGDGTAAAQALRNQEIDLYDGQPTAD
                     AVQILKAIPTAKIIGYSGAVYEHIDLRVGTAQGVTEPYTGPFAGDSQKARDLRTAFLM
                     AYPRDEIVDKLVKPINSKAVRMDSLMLFPGEPGYADMVKNSGVSKFTKGTQAEREAAA
                     LALVRKHSATDVKINMLWGQPSNVRRAAEAQIAKAALARAGFELNAPGRSGWSGFLDS
                     SEYDAQFFAWVKSALTAAGNTDIYKSDSGNNLVGYVNKTVDAAVTKLNATALSEKDRL
                     AQYLIVEKELMKDAISLPIFQHPGVTAVNKKLQNVKPNPLSPTLVWNYWEWKYSN"
     gene            2011..3555
                     /locus_tag="PLW53_05230"
     CDS             2011..3555
                     /locus_tag="PLW53_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36856.1"
                     /translation="MFAYILRRLGALVVILLGSSFLIYNLAAISTDPLEGLKTSTAQN
                     KEQLIASVTRELRLDLPPPLRYFLWLRGVLGIFVGNPNFGNTREQQPVVEAIANAIPT
                     TIRLVFVATIVALVLGLALGITSALRQYSRFDYGMTFFAFLLFSLPIFWVAVLLKQYL
                     AIDFNDFLVTAKISPPWIIGLSAITGFFWAAILSGSRRRVLMIFSGVFVANSIFLSLI
                     SATEWLSYPRLGPIAIFIFSIGIAFGITYLSVGLSDRNAVKSNLFMALIGTILYFPAQ
                     SLLDSNRPRVGILILLIALLISAVSVSFIFARIDRGPIIRTSIITSLLIGGLILIDKM
                     MQAWRPYVESDDVNYRPVATIGQSTIWLSDVTFWVRVLDFAMHLVLPTLALTLISFAG
                     YIRFSRGTLLDVLNQDYIRTARAKGLTERTVIMRHAFRNTMIPLTTIMVGDVAGIVGG
                     AIITERVFAWQGMGTLFNKAITSFDLNLLMGVILFLSTLAILANLIADLLYSALDPRI
                     RVGAGK"
     gene            3555..4643
                     /locus_tag="PLW53_05235"
     CDS             3555..4643
                     /locus_tag="PLW53_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36857.1"
                     /translation="MAKKKESVEVIKDAGENAIFNKETEGLSQGQIVLRRFVRHRAAM
                     ISVVVLFVLITVVYTALDWRIGDEEDPFVIPGWWKYGFEDMPELRYGADCVNGSVGCP
                     TFDAVPFFDGDGVSLGEHPFGQDDIGRDYLALVMRGAQRSLLVMFIIGAIAATLGTVI
                     GSIAGYFRGWVDAILMRIVDFIITIPVIIIGSVIGFHFGNKGAAFLALLLGLFSWTGL
                     SRLVRGEFLTLREREFVDASRVAGASNRRIIFKHILPNAVGVIIVSVTLLMSGAILLE
                     TALSYLGFGVTSPDVSLGLLISNYQDAFTTRPWLFWYPGLFIIVIALCINFIGDGLRD
                     AFDPRQRRRLSRKEKANARAMQSRSELA"
     gene            complement(4640..5239)
                     /locus_tag="PLW53_05240"
     CDS             complement(4640..5239)
                     /locus_tag="PLW53_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36858.1"
                     /translation="MSNVEQFRPRNGYFLSFWIFFTISTLTYVNVLDYGFLGGLPSIT
                     WGLFACSLFYLIFLHPKVVYCDEGLVIINPMQNITLGWDEISLIDSKFTMYVIHQSSG
                     AKYHAFAAQAPGRYHIRNVHPTEIRGMQLNDSGSIKAGESPRSNSGIAIAIARTRFEN
                     FHQRGNIQGLKFSIAFNRLGASIVLFLLLLAITTQLLQS"
     gene            5636..7489
                     /locus_tag="PLW53_05245"
     CDS             5636..7489
                     /locus_tag="PLW53_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD36859.1"
                     /translation="MKNKKLRVAIVGSLSILLAASTVTPPANAAKNGTVILHEPNPLS
                     GFNSAVVGFNLVTNSTIGYLTGMGFGYYDSSRTWVQNTTFGSYKVTSNKANDFRVQYT
                     VAPGRVWSDGTPITGIDLLSTHMIYSRDYAKAAGLGDWAAGDKMAFNAAGLSSSTYSL
                     YQTGDPVLSADEMSVTFKYKQKFPDWWLSVIGARPVHAWTLAAEGKTELQSVSANNAA
                     RDRWYAAYKAKDADLLKKIGTIWSTSYNSPDVNAATTNPLLWVGNGGFTVVSAVKGQS
                     VTLKANPLYNSGPKVSGDIKNIIQKFVADGNPVVQALGNGEVSLYSGQQTADGVAALK
                     KLAGVTTIGGLGGAYEHVDLRSGAYFSGGTPYTGLFAGNGQKATDLRRAFLLCVPRQE
                     IVAKLITPINSEIPPLRSVTVPSHEDSKYAKVIAANGSSYYVGTQESLNKRALSLVQK
                     YQKDAVKNPLKVNFLVPGNNARRAAQALLLKANLAKCGFDVNLDTQVDWSPKLRDSKY
                     DATFFAWAATSTAQGSIRANWRSDGSNNYSGANSGPGLDAILDDVFGRPMSDSVLSTA
                     FIKIEREIFGKAYTLPLYQHPAVTAYDNDLKGVKASSLSPVKEWNFWDWKY"
     gene            7638..9182
                     /locus_tag="PLW53_05250"
     CDS             7638..9182
                     /locus_tag="PLW53_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36860.1"
                     /translation="MLAFIARRIGVLFLILFGSSFILYNLAAIAGDPIGDLRFSDDPE
                     IQAQIRELEEFLRLDVPPPLRYFIWLRGVLGIFTGNLDLGLTRLRLPVSGEIATAMPI
                     TIRLVIFATIFAIIVGIALGIVTALRQYSRFDYSMTFFAFLLFSLPIFWVAVLLKQYL
                     AIQFNTFLSDPQITTSWKIGIGLVGSVFWASLLGSKRGNFWKVFGIVFVGTFAFLTFV
                     DEANWLTDPALGPVLIGLLAVGIAFGITYLSTGFGNKAALYASLTMAPLTVISYFVVS
                     NSLNSSSSIPQLLRYALITIVVAVAVALAFARIDRGPVVRTTVITALLSGHLIIVDKF
                     MQTWKPYMEEGAVSFRPIPTLGQAKELLTPNEFWISTLDILVHLFLPTIALTLISFAG
                     YIRYSRGTLLEVLNQDYIRTARAKGLNERTVIMRHAFRNTLIPLTTIMVVDFAGIVGG
                     AIITERVFGWYGMGTLFNRAITSQDLNLLMGVFFITATLAVMANLIADLLYSALDPRI
                     RVGSGK"
     gene            9179..10264
                     /locus_tag="PLW53_05255"
     CDS             9179..10264
                     /locus_tag="PLW53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36861.1"
                     /translation="MKIRRKSKEQAIEELTIESGENAIANKEVEGLTQWQIIRRRFFK
                     HKAAVASLIFLTFIIVFVFSAVDANFGPIKTKGWWKWSPEDLPELRFGDCPNDTIGCP
                     TISLVPKFLGGAGIGLGEHPFGQDDIGRDYFSLVMKGTQRSIQVMFIIGFFAAIIGTV
                     VGAVAGYYRGVVDNVLMRFTDFIITIPAVIVGSVIGAKFGNLGAIILAVYLGLFAWTG
                     LSRLVRGEFLTLREREFVDAARVAGASTRRIIFKHILPNAVGVIIVSVTLLLSGAILL
                     ETALSYLGFGVVPPDVSLGLLINQYQDAFTTRPFLFWYPGFFIVSIALSINFIGDGLR
                     DAFDPRQKRKITKKEKQNARAIARNQA"
     gene            complement(10255..10884)
                     /locus_tag="PLW53_05260"
     CDS             complement(10255..10884)
                     /locus_tag="PLW53_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRD36862.1"
                     /translation="MAYSPFSALVNNGGNLIIFRAQGSRVYSSLGYLFCLGMLITTVL
                     ESKYSSAENFAFCALILLIATSVYLGIQRPALVFTDLGLAVRNPFTTTILDWRQVSDL
                     DTKFVLNIKSDLGIIKCWAAAGPSRSQHRAIHPSELKGFHQQGGGIKAADSPRSDSGA
                     AAQIARRYIQERGSFKGSITSEIKVNNLAKFLYIALGITVIVSHLIAYA"
     gene            10883..12526
                     /locus_tag="PLW53_05265"
     CDS             10883..12526
                     /locus_tag="PLW53_05265"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36863.1"
                     /translation="MSEPILKVKDFSVDFWVDGLWYPAAIKMNFELHPGKVLAIVGES
                     GSGKSTTALGLMDLLASNARRSGQVLIKDVDMINVKSSTLRKFRGKEVAYIFQEPMTA
                     LNPVYTIGFQIVETLRNHFDMGPKQARTRAIELITMVEIPNPEQSFDKYPHQLSGGQR
                     QRAMIAQALACDPGLLVADEPTTALDVTIQAEILDLMRSLKDRLNSAILLITHDMGVV
                     ADMADEILVMKDGLTVEHGSSDQIFNRPKHPYTTELLAAVPKLGSSRKRVLKVARDAE
                     PVLKMRDVTIEYPKRGRIPAFTAVKNFSLDIFPGEIVGLVGESGSGKTTVGKAAIGLL
                     PIKSGSLEIVGKDISKASQKELFTIRRHTGIVFQDPASSLNPRLPIGESIGEPIFLAK
                     LAQGDELNKKVEDLLEQVELPRSYRNRFPHELSGGQRQRVGIARALALTPDLLIADEP
                     TSALDVSVQARFLDLLLDLQDKLKFACLFISHDLAVVDILAHRIAVMQNGILVEEGER
                     DQILNSAKDPYTQRLIAAVPVPDPAEQKKRREARLAGKK"
     gene            12529..>13583
                     /locus_tag="PLW53_05270"
     CDS             12529..>13583
                     /locus_tag="PLW53_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36864.1"
                     /translation="MTQPVSLEAFGISKEFGYMQHSDPVTSLSSANGAWDEMARNLPK
                     YLMGSDFRSRVKSLPPFKMDALASEGEVRRAMLALSYIGMAYQWSENDAAHVIPAVLA
                     KPWYELGVKVGRPPILSYVSYSIDNWYRLDPSKEIECGNIALLQCFLGGQDEEWFILI
                     HIDIEKKAGIALHAIELAQKAVVANDSDALDIALTQLRDGIKSMYDVLARMPEKCDPY
                     VYFHRVRPYIFGWRNNPALPNGVVYEGVEALGGKGMTYRGETGAQSAIVPALDGVLGI
                     EHERDELREYLMEMREYMPPKHVAFIEAVEAGPSVREFVKKVNRASTTELFNQSVELV
                     ADFRAMHLEYAGQYIHA"
BASE COUNT         3635 a         2975 c         3093 g         3880 t
ORIGIN      
        1 aatttctatc actaggagaa gaaatgaaat taaatcgcat tgccaaggta gcgatcgcta
       61 ctgcagctgc ggcgggtctt gcagtttcaa cgctgacacc agcgggcgct gcaactcgtt
      121 caactgtggt acttgttacc agcaatcaga tgacaagctt gaacagcgga acgcctgata
      181 ccaacctaac tgttaacagt gaaattggtt atctacaagg ctttggcttc aattattacg
      241 ataacgcacc aaagttggtt cgcaatacaa tttttggttc atacaaaatc gtaagccaaa
      301 atccgtttaa ggttcagtac acagtaaata agggcaaggt ttggtcagat ggaacaccaa
      361 ttactggtgt agatcttctg ctctcacacg ttcttgcttc aagctcatac tcattcaccg
      421 caaagctagg tgatccaaca gatcctgagg tcagaccagc atttaatgcc ctcggttatg
      481 gtggaattta tgaccagaca catgtcggtc taccaacact ttcagctgac aagatgtcag
      541 taacaattga gtacgattca aagattccag attgggaaat taccggtcct ggaccagcag
      601 cagtacatgc gctgattcac cttgcagaag gaaagaccga attgcagagc gcagctgcga
      661 atcttgctgc aaaggcaaag ttcgaaaagg cgttttataa ctacgatact aagttgatga
      721 aggcagcagg caaagtttgg tctgaggctt acaacatcac agatatcaat gattcaacta
      781 acccactact tctaattggt aatggtggtt acaagatcaa gagcgcagta actggtcaat
      841 cagttacttt ggttctaaac cctaagcacg tcactaaccc atcaggtcca aagacaaacg
      901 gagttaagac cattatcttc cgcgttgttg gcgatggaac ggctgcagct caggctcttc
      961 gtaaccaaga aatcgatcta tacgatggtc agccaactgc tgatgccgtt cagatcctaa
     1021 aagctattcc tactgcaaag atcatcggtt actcaggagc tgtttatgag cacatcgacc
     1081 ttcgcgtcgg aactgctcaa ggcgtaactg agccttacac cggtcctttt gctggcgata
     1141 gccagaaggc aagagatctt cgtaccgcat tcctaatggc atatccacgc gatgaaattg
     1201 tagataaatt ggtcaagcca attaactcca aggcagttcg catggattca ctaatgctat
     1261 tcccaggaga gccaggctat gccgacatgg tgaagaacag cggcgtatcc aagttcacaa
     1321 agggaactca agcagagcgt gaagctgcag cacttgcact tgttcgcaag cactcagcta
     1381 ctgatgtgaa aatcaatatg ctctggggtc agccaagcaa cgttcgtcgt gctgctgaag
     1441 cacagatcgc aaaggccgca ctagctcgtg ctggatttga gctaaatgca cctggaagat
     1501 ctggctggag cggattcctt gattcaagcg aatatgatgc acaattcttc gcatgggtta
     1561 agagcgcact aacagctgct ggaaatactg atatttacaa gagcgacagc ggtaacaacc
     1621 ttgttggcta tgtaaataag actgttgatg cagctgtaac caagctcaat gccactgcat
     1681 tgtctgagaa agacagactt gcacagtact taattgttga gaaggaattg atgaaggatg
     1741 caatctccct tccaatcttc cagcacccag gcgtgactgc agtaaataag aaactgcaga
     1801 acgtcaagcc aaacccacta tctccaacac ttgtgtggaa ctattgggag tggaagtact
     1861 caaactaagt agagatgctt agtttgaaaa attctcactc tttgtagtga gtatttagca
     1921 gtaattcgag tggcgcctaa gtagaattta ggcgccactt gaatcatgta gagccatcta
     1981 tctagatcta gcgggaaaag gaggctggaa ttgtttgcct atatcctcag gcgccttgga
     2041 gcattagtcg tcattctcct tggatctagt ttccttattt ataaccttgc ggcaatctca
     2101 actgatcctc tagaaggtct taaaaccagt actgcccaga ataaagagca gttgattgca
     2161 tcggtaacaa gagaactcag attagatctg ccacctccac tgcgttattt cctctggctg
     2221 cgtggcgtgc tgggaatttt tgttggaaat cctaactttg gtaatactcg tgaacaacag
     2281 ccagtggttg aagccattgc taatgcaatt ccaaccacta ttcgtctagt ttttgtggca
     2341 acaattgtcg ctctagttct agggctagct cttggaatta cgtcagcgct tcgtcaatat
     2401 tctcgatttg attatggaat gacattcttt gcattccttc tattctcgct gccaatcttt
     2461 tgggtggcag ttttgcttaa gcagtactta gctatcgatt ttaatgactt tttagtaacg
     2521 gcgaagatat ctccgccttg gattattggc cttagcgcca ttactggttt tttctgggct
     2581 gcaatactaa gtggctctcg cagaagagtt ctaatgattt tttctggcgt ttttgtggca
     2641 aattcaattt ttcttagttt gattagcgca accgagtggc tttcctatcc tcgcttgggc
     2701 ccaattgcta ttttcatatt tagtattggt attgccttcg gaatcacata tctctccgtc
     2761 ggtctttcag accgtaatgc tgtcaagagc aatctcttta tggctttaat tggcactatt
     2821 ctttatttcc cagctcagag cctgcttgat tcaaatcggc caagagttgg aattttaatt
     2881 ttgttaattg cccttttgat ttcggcagtt tcagtttcat ttatttttgc ccgtatcgat
     2941 cgtggcccga ttatccgcac cagcattatt acctcgctat taattggcgg cttgattttg
     3001 atagataaaa tgatgcaggc ctggaggccc tatgttgaaa gcgatgatgt taactatcgc
     3061 cctgttgcca caatcggaca gtcaacaatc tggctatctg atgtgacatt ttgggtcagg
     3121 gtcttggatt ttgcgatgca cctggtgcta ccaacacttg ccttaacgtt aatttccttt
     3181 gctggatata tccgattctc ccgcggcact ctgctggatg ttttaaacca ggattacata
     3241 agaacagcaa gggctaaagg cttaactgag cgtactgtga ttatgcgcca tgcctttaga
     3301 aacaccatga ttccgctcac cacaattatg gttggcgatg tagctggaat tgttggagga
     3361 gcaatcatta cagagcgagt atttgcttgg cagggaatgg gaacgctatt taataaggca
     3421 attacctctt ttgatttaaa tcttttaatg ggagtaattc tcttcctttc caccttggca
     3481 attctggcaa atttgattgc ggaccttctc tattcagccc ttgatcctcg aattcgcgtg
     3541 ggggctggta aataatggca aagaaaaaag agagcgttga agtcattaag gatgctggcg
     3601 aaaatgccat cttcaataaa gagaccgagg gcctaagtca gggtcagatt gttctgcgtc
     3661 gctttgttcg ccatagagct gccatgattt cggtagttgt gctatttgtc ttgataacag
     3721 tggtctatac cgcacttgat tggcgcattg gtgatgagga agatccattt gtcatccctg
     3781 gttggtggaa atatggcttt gaagatatgc cggaacttcg ctatggagcg gattgtgtta
     3841 atggctcagt tggatgcccc acctttgatg ccgttccctt ctttgatggc gatggtgttt
     3901 cacttggtga acatccattt ggtcaggatg atattggtag agattattta gctcttgtga
     3961 tgcgtggagc ccagcgctca cttctagtta tgttcataat tggcgcgatc gcagcaaccc
     4021 ttggaacagt aattggatca attgctggat atttccgtgg ttgggttgat gcgattttga
     4081 tgagaatcgt tgacttcatt attacgattc cagtaatcat tattggttca gttattggtt
     4141 ttcattttgg caataaggga gcagccttct tagctctgct cttaggacta ttttcctgga
     4201 ctggactttc gcgcttggtt cgcggggagt ttctaacact tcgagaacgt gagttcgtcg
     4261 atgcctcaag agtggctgga gcaagtaatc gacgcatcat tttcaagcat attcttccta
     4321 atgctgtggg agtaattatc gtttccgtaa cgcttttgat gtctggcgcg atattgcttg
     4381 aaacagccct gagttatctt ggttttggag tcacctcacc tgatgtttcc ctaggactat
     4441 tgatctccaa ctaccaagat gcctttacca ccaggccatg gctcttctgg tacccaggcc
     4501 ttttcatcat tgttattgct ctatgtatca actttattgg cgatggactt cgcgatgcat
     4561 ttgatccaag acagcgtcgt agattaagtc gtaaagagaa agctaatgct agagcgatgc
     4621 aaagtagaag cgagcttgct taggattgca gcaattgagt tgtaattgct aataggagta
     4681 gaaatagcac aatagaggca ccgaggcgat taaaggcaat ggaaaacttt aatccttgaa
     4741 tattgcccct ctgatgaaaa ttctcaaaac gagttctagc aattgcaatt gcaattcctg
     4801 aattacttct tggactttct cctgccttaa tgctgccgct atcgtttaat tgcatacccc
     4861 ttatttcagt tggatggaca tttctgatgt gatatcggcc aggggcttgg gctgcaaatg
     4921 cgtgatactt agcgccgcta ctttgatgga ttacatacat tgtgaatttt gaatcaatta
     4981 atgaaatctc atcccaaccc aaagtaatgt tctgcatagg attgataatt acaagacctt
     5041 catcacaata aacaactttg ggatggagaa agattagata gaagagcgag caggcaaata
     5101 acccccaagt gatactggga agtccgccaa gaaagccgta atccaataca tttacataag
     5161 ttaaagtgct aatcgtgaag aagatccaaa atgaaaggaa atatccattt ctcgggcgaa
     5221 attgctcgac gttactcacc gctcaatctt cccatctaaa ttggcgaata ctttcaaagg
     5281 atcaaaattg cctgagttct gcgttaacct ccacggtcaa gaaacgagtt ttttaaacct
     5341 gcaaatgatg tggcctgctc agggctaagt aaagatattt ctgggggata ctggctctca
     5401 gggcgagccc agaatcagga aattacagcc caacgcctct gaaatagcgc ttggattaac
     5461 caaaagccaa ggctgaaaat gataaagata ttgttatcta gccttcttcg gggcaattgt
     5521 cagaaatagc cctataaggc agactttgcc aaccctcgag ggttagttaa tggtaaagcg
     5581 gtcgactctt tactaatgac tatccccgca aacaaagacc ttttaggagg ttatcttgaa
     5641 aaacaagaag ttaagagtgg caattgttgg atcactttcg atccttcttg ctgcttcaac
     5701 agtcacgccg ccagcaaatg cggctaagaa tggaactgta attctccatg agccaaaccc
     5761 tctatcaggc tttaactcag ctgttgttgg tttcaacttg gtaacaaact ccaccatcgg
     5821 ttatctaacc ggtatgggat ttggatatta cgattcctca agaacttggg tacagaacac
     5881 cacatttggt tcttacaaag taacttcaaa taaggccaat gacttccgtg ttcagtacac
     5941 cgtagctcca ggaagagttt ggtcagatgg aactccaatt actggaattg accttctctc
     6001 aactcacatg atttatagcc gtgattatgc aaaggctgca ggtctaggtg actgggctgc
     6061 tggagataag atggcattta atgctgctgg tctttcttca agcacctact ctctatatca
     6121 gacaggcgat ccagtactaa gcgctgatga aatgtcagtt actttcaagt acaagcagaa
     6181 gttcccagat tggtggctat ctgttatcgg tgcacgtcca gtacatgctt ggacgctagc
     6241 tgctgaaggt aagactgaac tacagagcgt ctctgcaaac aatgcagcac gtgatcgttg
     6301 gtatgccgca tacaaggcta aagacgctga tcttctaaag aagattggaa caatctggtc
     6361 aacttcttat aacagcccag atgttaacgc tgcaacaact aacccacttc tatgggttgg
     6421 aaatggtggc ttcaccgtcg ttagcgcagt taagggtcaa tcagtaacct tgaaggcaaa
     6481 cccactttat aactcaggac ctaaggtctc aggagatatt aaaaacatca tccagaagtt
     6541 cgtagctgat ggaaacccag tggttcaggc ccttggtaat ggtgaagttt ctctctactc
     6601 aggtcaacag acagctgacg gagtcgctgc tttgaagaag cttgctggcg taaccacaat
     6661 tggtggtctc ggcggagctt atgagcacgt agatcttcgc agtggtgctt acttctcagg
     6721 tggaacgcca tacaccggtc tatttgccgg aaatggacag aaggcaactg acctacgtcg
     6781 cgccttccta ctctgcgttc ctcgtcaaga aattgttgcg aagttgatca ctccgattaa
     6841 ctcagagatt ccaccacttc gctcagtaac agttccatca catgaagata gcaagtatgc
     6901 aaaagttatt gctgctaatg gatcttcata ttatgttgga acacaagagt cactaaataa
     6961 gcgcgctctc tcactcgttc agaagtatca aaaggacgct gtaaagaatc cgcttaaggt
     7021 gaacttccta gttcctggaa acaatgcacg tcgtgcagcg caagcgctat tgcttaaggc
     7081 aaaccttgct aagtgcggct ttgatgtaaa ccttgatact caagttgatt ggtcaccaaa
     7141 gcttcgcgat tccaagtatg acgcgacctt ctttgcctgg gctgcaacca gcactgcgca
     7201 aggatcaatt cgcgctaact ggcgctctga tggctccaac aattacagcg gagcaaactc
     7261 aggtcctgga ctagatgcga ttttggatga tgttttcggt cgtccaatgt cagattcagt
     7321 tctaagcaca gcctttatca agattgaacg tgaaatcttt ggtaaggctt acacactacc
     7381 tctataccag catccagcag tgactgccta tgacaacgac cttaagggcg ttaaggccag
     7441 ctcactatct cctgtgaagg aatggaactt ctgggattgg aagtactaaa aaagaagtag
     7501 agctaatcgc tcaactttga aggcttgtag ggccttaggg atttttaacg aaattaccta
     7561 aggcccacag gctttatttt tggctaaaat gagtaaaatc ctcacgcttt agaaactaac
     7621 taggaaaggc gagtgccgtg ctagctttca ttgctcgacg aatcggcgtt cttttcctta
     7681 tcctttttgg ctcaagcttt atcctctata accttgcagc aattgcagga gatccaatag
     7741 gtgatcttcg ctttagcgat gatcccgaga ttcaagctca aattagagag ctagaagagt
     7801 ttttaagact cgatgtgccg cccccactta gatatttcat ctggctacgc ggcgttctag
     7861 gaattttcac cggcaatctt gatttagggc taactcgtct tcgtcttcca gtatcaggcg
     7921 aaattgcaac tgcaatgcca atcactattc gactagttat tttcgcaact atatttgcaa
     7981 tcatcgtggg tattgcccta ggtatcgtta cagctcttcg tcaatattca cgttttgatt
     8041 attcaatgac attctttgca ttccttctct tttcactacc tattttctgg gttgcagttc
     8101 ttttgaagca gtacttagca attcagttca atactttctt atctgatcct cagataacta
     8161 cctcatggaa aatcggaatt gggctagttg gctctgtttt ctgggcctca ctgcttggtt
     8221 caaagcgcgg aaacttttgg aaggtctttg gaattgtctt tgtgggaaca tttgcattcc
     8281 ttacctttgt tgatgaagct aattggttaa cagatccagc acttggccca gtattaattg
     8341 gtctgctggc tgtcggtatt gcatttggaa ttacttactt aagcactgga tttggcaata
     8401 aggcagcgct ctatgcctct ctaacaatgg ctccactaac tgtcatctct tattttgtgg
     8461 tctcaaactc gcttaattct tcatcttcaa ttccgcagct acttagatat gcgctaatca
     8521 caatcgttgt tgcggtggcc gtggctcttg cctttgctcg aatagatcgc ggaccagttg
     8581 ttagaactac agttattact gctctgctct cggggcactt aattattgtt gataagttca
     8641 tgcagacttg gaagccatat atggaagagg gagcggtaag ttttaggccg attccaactc
     8701 ttggccaggc aaaggagctg ctcactccaa atgagttctg gatttcaacc ctagatattt
     8761 tggtgcactt attcttgcca actattgctc ttaccttgat ttcatttgct ggctatatcc
     8821 ggtattcaag aggaactctg cttgaagtac taaatcagga ttacatcaga actgctcgcg
     8881 caaaagggct aaatgagcga actgtgatta tgcgccatgc cttccgcaat actttaattc
     8941 cattaaccac aattatggtg gttgattttg ctggaattgt tggtggcgca attattactg
     9001 agcgcgtctt tggttggtat ggcatgggaa cgctctttaa tcgagcaatt actagccaag
     9061 atctcaacct gctaatgggc gttttcttta ttactgcaac actagctgtg atggctaact
     9121 taattgcaga ccttctctac tcagcccttg acccaagaat tcgcgttggg agtggaaagt
     9181 gaaaatcaga agaaagagta aagagcaggc cattgaagag ctaacgattg agtctggcga
     9241 aaacgctatt gccaataagg aagttgaagg attgacgcaa tggcagatca ttcgccgacg
     9301 tttctttaaa cataaagctg cagtagcctc acttatcttc ttaactttca ttattgtctt
     9361 tgtattttcc gcagtagatg caaactttgg cccaataaag actaagggtt ggtggaagtg
     9421 gagcccagag gatttgcctg agcttcgatt tggtgattgc cctaatgaca ccattggctg
     9481 tccaacaatt tcacttgttc caaagttttt agggggagct ggaattggcc ttggtgaaca
     9541 cccatttggt caggatgaca ttggtcggga ttacttctcg ctggtaatga agggcacgca
     9601 gcgctctatt caagttatgt tcattattgg attctttgca gcgattatcg gaactgttgt
     9661 tggcgccgtt gcgggatatt accgaggcgt tgttgataac gtccttatgc gcttcaccga
     9721 cttcatcatc acaattccag cagttatcgt gggatcagtt atcggtgcaa agtttggaaa
     9781 tcttggcgca atcattcttg cggtttattt gggtctcttt gcttggacgg ggctatctcg
     9841 tctggttcgt ggcgagtttt taacccttcg tgagcgagag tttgtcgatg cggcgcgagt
     9901 tgctggagca tcgactagaa gaattatctt caagcacatt cttccaaatg ccgttggtgt
     9961 aatcatcgtc tcagttacat tgctgctctc gggagctatc ttgctagaga ccgccctttc
    10021 ctaccttggc tttggggttg tgccccctga tgtttccctt ggacttttga ttaatcaata
    10081 tcaggatgct ttcaccacca ggccattcct attttggtat ccaggattct ttattgtaag
    10141 tattgcctta agtattaact tcattggtga tgggctaaga gatgcctttg atccaaggca
    10201 gaagcgcaag attacaaaga aagaaaaaca gaacgctaga gctatcgcaa gaaatcaggc
    10261 gtaggcgatc aaatgcgaaa caattacagt aattccaaga gctatataga ggaatttagc
    10321 cagattatta actttaattt cactagtgat acttccctta aaacttccgc gctcctggat
    10381 atagcggcga gcaatttgag cggcagctcc agaatcagat ctaggcgagt cagcagcttt
    10441 aattccaccg ccttgttgat gaaatccttt gagttcactg ggatgaatag ctcggtgttg
    10501 agatctagag ggtccagcag cagcccagca tttaatgatt ccgagatcac tttttatgtt
    10561 gagaacaaat ttagtatcaa gatcactaac ctgcctccaa tcaaggattg tggtggtgaa
    10621 ggggtttcta actgccagtc caaggtcggt gaagaccaga gcaggtcttt gaattccaag
    10681 gtagaccgag gtagcaatca agaggataag agcgcaaaag gcgaaatttt cagcgctgct
    10741 gtatttggat tcaaggactg tagtaatcag catcccaaga cagaagagat atcccaaact
    10801 actgtaaact cgcgagcctt gggcacggaa aataatcaga tttccaccgt tgttaaccag
    10861 agcgctgaat ggagagtaag ccatgagtga gccaatccta aaggttaaag atttctcggt
    10921 cgatttctgg gtcgatggcc tctggtatcc agcagccatc aagatgaact tcgagctcca
    10981 cccaggcaaa gtccttgcca tcgttggtga gtcaggttct ggtaaatcaa caaccgccct
    11041 tggtctaatg gatctacttg catcaaatgc aagacgaagc ggacaggttc taattaaaga
    11101 tgtagatatg atcaatgtga agagttcaac gcttcgcaaa ttccgcggca aggaagttgc
    11161 ctatatcttc caggagccaa tgactgcgct aaatccggtc tacacaattg gattccagat
    11221 tgttgagact ctacgaaatc actttgatat ggggccaaag caggctcgca cccgcgctat
    11281 tgaacttatt acaatggttg aaattccaaa cccagaacaa tcatttgata agtatcctca
    11341 ccaactatca ggtggtcagc gccagagagc aatgattgct caagcacttg cctgtgaccc
    11401 aggtctttta gtggcagatg agccaaccac cgcacttgat gtaacgatcc aggccgaaat
    11461 cttagatttg atgcgaagcc tcaaagatcg acttaactca gcaatccttc ttatcaccca
    11521 cgatatgggc gttgttgctg atatggcaga tgagatcttg gttatgaaag atggactcac
    11581 cgttgaacat ggcagctctg atcaaatctt taatcgccca aagcatccct atacaactga
    11641 gctattagct gcagttccaa agcttggttc ctcaagaaag cgcgttctta aagtcgcaag
    11701 agatgccgag ccagttctta agatgcgcga tgtaacaatc gaatatccaa agcgaggaag
    11761 aattccagcc tttactgccg ttaaaaactt ctcactggat atcttccctg gagaaatcgt
    11821 tggtctagta ggcgaatctg gctctggaaa gaccacagtt ggaaaagcgg ctattggatt
    11881 gcttccaatt aagtcaggaa gccttgagat tgttggcaaa gacattagta aggcctcaca
    11941 aaaagagctc tttactatcc gccgccacac cggaatcgtc tttcaagatc cagcttcatc
    12001 acttaaccca cgtcttccaa ttggtgaatc cattggcgag ccaatattcc ttgcaaaatt
    12061 agctcaaggt gatgagctaa ataagaaggt tgaggatctc cttgagcagg ttgaacttcc
    12121 tcgctcctat agaaaccgct tccctcatga gttatcaggt ggccagagac agcgagttgg
    12181 aattgcaaga gctctggctc taaccccaga tctattaatc gctgatgaac cgacctcagc
    12241 acttgatgtt tcagtacaag cacgcttcct agatctcttg cttgatctgc aagataaatt
    12301 gaagtttgcc tgcctcttca tcagccatga tctagcagtt gttgacatct tggctcaccg
    12361 aattgcagtt atgcaaaatg gaatcttggt tgaagagggc gagcgagatc agattcttaa
    12421 tagcgctaaa gatccttata ctcagcgtct tatcgctgca gtgcctgtgc cagatcctgc
    12481 agagcagaag aagagacgcg aagctcgctt agctggcaag aaataattat gactcagcca
    12541 gtttcactag aggcctttgg catctcaaaa gaatttggct atatgcagca cagcgatcca
    12601 gtcacctcac tctcttctgc aaacggtgct tgggatgaga tggcacggaa tctgccaaag
    12661 tatttaatgg gctcagattt tagatcccga gttaaatcac tgccaccttt caaaatggat
    12721 gcgctagcaa gcgagggcga agtcagacgt gcaatgctcg ctctttccta tattggtatg
    12781 gcctatcaat ggtcagaaaa cgatgcagcc catgtaattc cagcagttct tgcaaagcct
    12841 tggtatgagc ttggggtcaa agttggtcgc ccgccaattc ttagttatgt ctcatactcc
    12901 attgataact ggtatcgcct cgatccaagc aaggaaattg aatgcggaaa tatcgccctc
    12961 ttgcaatgct ttcttggggg acaggatgaa gagtggttca ttctcattca catcgatatc
    13021 gagaagaaag ctggaatcgc gcttcatgcc attgaactag cccagaaggc agttgttgct
    13081 aatgacagcg acgcactaga tatcgctctt actcaactaa gagatggcat caagagcatg
    13141 tatgacgttc ttgccagaat gccagagaag tgtgatccat atgtttattt ccatagggtc
    13201 aggccatata tctttggctg gcgcaataac ccagctcttc caaatggcgt tgtttatgaa
    13261 ggcgttgagg cacttggcgg caagggaatg acttatcgag gagagactgg cgcccagagc
    13321 gcgattgttc cagcactaga tggagttcta ggaattgaac atgaaagaga tgagcttcgg
    13381 gagtatttaa tggagatgcg cgaatatatg cctcccaagc atgtggcatt tattgaagct
    13441 gttgaggcag gtccatcggt gcgggagttt gttaagaagg tcaatcgcgc ctcaacaaca
    13501 gagctcttta atcaatctgt tgagctagtt gcagatttta gagcgatgca tcttgaatat
    13561 gccgggcaat atattcatgc cca
//
LOCUS       DAOROD010000026        11621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_293_length_11621_cov_32.165053, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000026 DAOROD010000000
VERSION     DAOROD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_11621_cov_32.165053"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLW53_05275"
     CDS             complement(<1..710)
                     /locus_tag="PLW53_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HRD36865.1"
                     /translation="MATWLYLMRIVLALSLFTAVGITPLSAAGRTNKSLANTILSGKA
                     VPTSKVGIDGDFYINTNTFQIYGPKVNNRWPAPISLIGPTGSAGSDGKQGDKGSSTSG
                     ATGSKGEKGEKGDKGDTGEKGEKGEKGEVGAVGAKGDTGLTGATGLTGATGLTGATGL
                     TGATGLTGATGLTGATGPIGATGLTGPTGATGATGATGSSGLAGATGATGPTGATGAT
                     GAAGATGATGATGPSNVQV"
     gene            971..1510
                     /locus_tag="PLW53_05280"
     CDS             971..1510
                     /locus_tag="PLW53_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36866.1"
                     /translation="MKKRLIAGSAIAALALSLGSTTGAVADDKFRDGKGISNIFSGLV
                     KNGTLTQAQVDAISKAMQDARGAGKAAYEAAKAERIKVITDALGIDAATLEAKRKAGQ
                     TLAAIAGDKKDALIAALVAYESKKIDAAVADGKLSAERATALKSKLTAGITAMVNNEA
                     KIGKAFKGFGKKGHGRGGK"
     gene            1608..2063
                     /locus_tag="PLW53_05285"
     CDS             1608..2063
                     /locus_tag="PLW53_05285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HRD36867.1"
                     /translation="MRIHIGSDHAGLEFKAKIIAHLQSQGHQVTDHGPHSFDPLDDYP
                     EFCIPAALATAKDPGSFGIVLGGSGNGEQMAANKVKGVRAALVWSVEIAKLARQHNDA
                     NVISLGGRMHDEKLCLELVDTFLSTPFSNDERHIRRIGLISKYEEKGQL"
     gene            complement(2060..2737)
                     /gene="trmB"
                     /locus_tag="PLW53_05290"
     CDS             complement(2060..2737)
                     /gene="trmB"
                     /locus_tag="PLW53_05290"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRD36868.1"
                     /translation="MASQKSEEFRLRSFRLRGERMTKAQAGAIDEHLGEYEIPLDRPI
                     ELSKIFPKDRYKEIVCEIGSGMGEASSQIAFENPESGYLAVEVHKPGIGALLIRANNL
                     GIKNLRVINEDIYLVLRDYIPDSSFDAFHIFFPDPWPKRRQHKRRLLSPEFIETLAAK
                     LKPGGRINVATDWQPYAKEIESNFASSKSFNGGVIQRPTWRPLTKFESKGLGKEHLVT
                     DLQYFKN"
     gene            2774..5314
                     /gene="pepN"
                     /locus_tag="PLW53_05295"
     CDS             2774..5314
                     /gene="pepN"
                     /locus_tag="PLW53_05295"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRD36869.1"
                     /translation="MPGNNISRAEAAERSSHLSIKHYEVLLDVSGGAESFIATTKVSF
                     DCNKVGYESFIDAVGKRIISATLNGQSVDVSNYDGESVFLKNLQASNELVIEIEAIYS
                     KSGEGLQRSVDPVDNEVYLYSQGETAFIRNMYPCFDQPDLKATFAFTAIAPAHWQVIS
                     NNPVKQVSDLGDKKKWEFTTTPIMSTYITALVAGPYSFIHDKYEGKKSIPLGIYCRKS
                     LFEHLDSDEIFLITKQGFAYFEEVFGLAYPFDKYDQIAVVDFNWGAMENAGAVTFRED
                     LLVFRSKVTERMYNARANTILHEMAHMWFGDMVTMKWWDDLWLNESFAEWSSYLALVE
                     ATKFKNSWAGFNAERKNWAYRQDQLSSTHPIATDMVDIDTVKANFDGITYAKGASVLH
                     QLVAHVGRDNFIKGLQKYFAKHAFKNTVLSDLLVELEATSGRDLSSWVSTWLQTSGVN
                     TLRPVIETDGESYKSIAIKQEAPKIPAGSTELRPHRAAVGLYDLKDGQLLRRKSVELD
                     ISGDLTQVKELSGEKLADLLLINDHDLTYAKIRFDARSIATLKAHLGKLDDGLSRALC
                     WSAAWDMFRDAELSATDFIEIALTGLAGEDDITTVTALMTQLNTAVEIYASDKNRDSL
                     REKVAGALLSLLKSAKAGSDHQLQFARSFAALAHSKEQGAEIRNILDGKFQDLKIDAD
                     LRWHLILCLVERGLSTRAEIDAELKRDNTLTGALSHERCIAAFPTLEAKEAAFKKATE
                     DESITNWSRLSAIQGYNRPIQRHLHEGFVDRYFDLILETYNTKSYEVSTSIIDLLYPS
                     YLISSQTLAKTDAWLNGPGKDSHPTLRRHILEAKDSLERALRVRGIDN"
     gene            complement(5315..5515)
                     /locus_tag="PLW53_05300"
     CDS             complement(5315..5515)
                     /locus_tag="PLW53_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36870.1"
                     /translation="MSIQLALNEDGSMPGEPLAFLEGFLWFFVTPVSITLFIWVAVVA
                     LENVKKNKANRAPSDVITRIGE"
     gene            complement(5526..6053)
                     /locus_tag="PLW53_05305"
     CDS             complement(5526..6053)
                     /locus_tag="PLW53_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRD36871.1"
                     /translation="MNSSSRRTLVLNATYEPLGVVSDRRALILILNNRATMIEESGVT
                     LHYSSGSMELPAVIRLHKFVKIPYRHTVPLSRRAIFARDGGRCVYCESAATSIDHVLP
                     RSRGGDHSWENVVSACHRCNHVKADRTLKELGWRLRSIPREPVGAAWRILGTARAEER
                     WVRYLAPFGVVGASA"
     gene            6302..6727
                     /locus_tag="PLW53_05310"
     CDS             6302..6727
                     /locus_tag="PLW53_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRD36872.1"
                     /translation="MAEADFIPGEKRSVTRQRERAKTAASVLNSSSKAVIGLIAGAMI
                     LGELGVDLGPLVASAGVIGFAVGLGAQSIVRDVFSGIIMLLEDQYGVGDSVEVLEVKG
                     RVESVGLRVTTVRDRRGTLWYLRNGEILKVGNSSQKSRS"
     gene            complement(6709..7086)
                     /locus_tag="PLW53_05315"
     CDS             complement(6709..7086)
                     /locus_tag="PLW53_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HRD36873.1"
                     /translation="MSLYEQMGGRKTFENLVSHFYALVATDPILRPMYPDDDFKGAAE
                     RLLMFLEQYWGGPNTYSQLRGHPRLRMRHAQFKIGEKERDVWLSHMKSAVDSLEMDNE
                     MREQLWSYLVMAANSMVNQERDF"
     gene            complement(7095..8312)
                     /locus_tag="PLW53_05320"
     CDS             complement(7095..8312)
                     /locus_tag="PLW53_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36874.1"
                     /translation="MSSNTALQEKTVKVLATAQVLNGVGVAGTVAAGSLLVASITNSE
                     TLAGLAQTSSVLGAAALALPLARLTSRGGRRLALSVGLIAGVIGSLLAIFGGSRENIY
                     LMLTGTFLVGAASAAGYQARFAAIDLATNEKRAKQLSFVVWGSTIGAVTGPNLMEPSG
                     NLAESFGLPRLTGPYLISAVTLALAVLVIQLFLRPDPYLTAAKDSGAATLPRVKTKVA
                     LKHIRSNARASFAIASIAIGHIAMVAVMVMTPVHMAHVDVTLTIIGLVISVHVLGMYA
                     FSPLVGALTDRLGRLRVIQIGVAILLSSALISGFARADDAITLGIGLFLLGLGWSCTL
                     IAGSALLTESVSPEFKSASQGASDLVMNLSGASGGAIAGVVIATLSYGWLCLLSAIPV
                     ALLGLWSLKIKAR"
     gene            complement(8309..10015)
                     /locus_tag="PLW53_05325"
     CDS             complement(8309..10015)
                     /locus_tag="PLW53_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRD36875.1"
                     /translation="MSSKLAPRGQRPWWRDAVTYQIYIRSFADSNGDGIGDVGGIRSK
                     LGYLKKLGVDAIWITPWYPSPQKDHGYDVADYLDIEPDYGTLKEGELLIKEAHDLGIK
                     VIIDIVPNHTSDQHKWFKEALAAKPGSAARDRYIFREGKGKNGELPPNNWQAVFGGPA
                     WKRVTESDGKQGQWYLHLFAVEQPDLNWENNEVVKHFEDVLKFWLDKGVDGFRIDVAH
                     GMFKESGLPDVRSSWIEKIFGKNNLTRMLSPEHKPFWDQEGVHDIYRSWRKILDSYDG
                     DRMAVAEAWVSPASRIAKYVRSDELQNSFNFEMLTTLWKADEIRKKINNSIDALAEVG
                     APTSWVFNNHDVVRSVDRLDLGLTNHGDTTFSRHGDVNKLDIARGTLRAKSATLMALA
                     LPGGTYLYQGEELGLPEVRDIPEDRLTDPRWKMSNYKDRGRDGCRVPIPWKSSSGGAF
                     GFSSNENLTPEQAWLPPSTWWGRYSAESQENDPNSTLNVYRKALAIRKNEPGLGDGTM
                     AWIDTPKQVVAFRRGEDFACYINFGSEIELPENTQVLHSSAPLNGNFLPTDTAVWLRV
                     SK"
     gene            complement(10012..>10308)
                     /locus_tag="PLW53_05330"
     CDS             complement(10012..>10308)
                     /locus_tag="PLW53_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRD36876.1"
                     /translation="YSHVSKGEVPGILEMVVARAEIDFIAPILQGGFFVDVELWVESI
                     GNSSFVMGYEIKSGDTIYARIKSVQVGIDKQSGKSRPLEASERAFLEKYLEVKS"
     assembly_gap    10309..10408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10409..11613)
                     /gene="ettA"
                     /locus_tag="PLW53_05335"
     CDS             complement(<10409..11613)
                     /gene="ettA"
                     /locus_tag="PLW53_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRD36877.1"
                     /translation="MAEFIYTMKKARKAHGDKVILDDVTLMFLPGAKIGVLGPNGAGK
                     STVLQIMAGLQQPSNGEAYLSPGYSVGILLQEPPLSEDKNVLENVQEGVAETIALLDR
                     FNKVSEEMASPDADYDKLLAEMGSLQEKLDHLSAWDLDSQLEQAMDALRCPPPDADVK
                     VLSGGERRRVALCKLLLQKPDLLLLDEPTNHLDAESVLWLEQFLSKYEGTVVAITHDR
                     YFLDNVAQWILELDRGRAYPYEGNYSTYLETKAARLKVEGQKDAKRAKRLAEELDWVR
                     QNAKGRQVKSKSRLARYEEMAAEAEKARKLDFEEIQIPMGPRLGNIVVDVENLSKGFG
                     DRLLIDDLSFSLPRNGIVGVIGPNGAGKTTLFKMILGLETPDSGSIKVGETVKVSYVD
                     QSRSGIDPK"
BASE COUNT         3205 a         2647 c         2450 g         3219 t
ORIGIN      
        1 acttgaacat ttgaaggccc agtagctcca gttgcgcccg tggcacctgc tgctcctgtt
       61 gcaccagtag cacctgttgg tcctgttgca ccagtggcac ctgctaatcc agaagatcca
      121 gttgctcccg ttgcaccagt ggcacctgtc ggtccagtta atcccgttgc accaatgggt
      181 ccagttgcac cagttaatcc cgtcgcacct gttaatcctg tggcacctgt taatcccgtc
      241 gctccagtta atcctgttgc acctgttaat ccagttgctc cagttaaacc tgtatcacct
      301 tttgcaccaa ctgctccaac ttcgcctttt tctcccttct ctcctttctc gccagtatcg
      361 cctttatctc ccttttcacc tttctcacct tttgatccag tagcaccact agtacttgat
      421 cctttatctc cttgtttgcc atcacttccc gcacttccag ttggcccaat aagacttatt
      481 ggtgctggcc aacgattatt aacttttggc ccatagattt gaaatgtatt tgtgttgata
      541 taaaaatctc catcaattcc aactttagaa gttggaacag ccttgccact taagatggta
      601 ttggctagag acttattggt tctgccagcg gcagatagtg gcgtgattcc cactgcagta
      661 aataatgaaa gcgccaaaac gatgcgcatg agataaagcc atgtagccat aaatacccct
      721 cctaataggg ggtaaggacc ccttagttag ggatatggag ctatagcctg gcaaggcctt
      781 agatgaatat gtagaactat ttagctatat caatttctcc catctggccg tataagtagc
      841 gcgtgataga agaaaaactt taagccctct ttcaggtaat tgtcctacct atctcaggtt
      901 gatagattta catcatacct atcaggggca agggcccttg attagatgaa tgagtgaagt
      961 aggagtatcg atgaagaaaa gattaattgc aggatctgca atcgcagcac ttgctctctc
     1021 acttggcagt actacaggcg ctgtagctga tgacaagttt agagatggta aaggaatttc
     1081 aaatatcttc agtggacttg ttaaaaacgg aactttgaca caagcacaag tagatgcaat
     1141 ttctaaagcg atgcaggatg caagaggcgc aggcaaagct gcgtatgaag cagcaaaagc
     1201 tgagcgaatc aaagtaatta ctgatgctct tggcattgat gcagcaactc ttgaagccaa
     1261 acgtaaggca ggtcaaacac ttgccgctat cgcaggcgat aagaaagatg cgttgattgc
     1321 tgctctggtg gcatatgaaa gtaagaaaat cgatgctgcc gtagctgatg gaaagctaag
     1381 tgctgagcga gccactgcgc tcaaatcaaa gttaacagct ggaattactg ccatggtcaa
     1441 taacgaagcc aaaattggca aggcttttaa aggctttggc aaaaagggtc acggacgtgg
     1501 cggcaaataa ggctttagcc tagataagtt catttctctc ccccttcgta taagaaccgc
     1561 tggtagaaat gccagcggtt cttattttta tttgagaagg tagccctatg aggattcata
     1621 ttggcagtga tcatgccggg ctggaattta aagcaaaaat catcgctcat cttcaaagcc
     1681 aaggccatca agtaacagat catggcccgc atagctttga tccgctcgat gactatccag
     1741 aattctgtat tccagcagcg cttgcaactg caaaagatcc gggctctttt ggcattgttt
     1801 tagggggctc aggaaatggc gagcagatgg cagcaaataa agttaaaggg gtaagagcag
     1861 cgctggtttg gagcgttgag attgcaaagc ttgctaggca gcataacgat gccaatgtga
     1921 tatctcttgg cggcagaatg catgatgaaa aactctgttt agagctagtg gatacctttc
     1981 tttcaacacc ttttagcaat gacgagcgcc atataagaag aattggttta atttcaaagt
     2041 atgaagaaaa aggccagctt taattcttaa agtattgcag atcagttact agatgctctt
     2101 tgccaagacc cttggattca aacttagtta agggcctcca tgttgggcgc tggattactc
     2161 caccattaaa acttttacta cttgcaaagt ttgactctat ttcttttgca taaggctgcc
     2221 aatctgttgc aacattgatt cgaccaccag gttttagctt tgcagctaag gtctctataa
     2281 attcagggct taagagacga cgcttatgtt gacgccgctt tggccatgga tctggaaaga
     2341 agatatggaa ggcatcaaag cttgaatctg ggatgtaatc ccgtaagact aaatagatat
     2401 cttcattgat aactcttaaa ttcttaattc caagattgtt tgcgcgaatt agcagggctc
     2461 cgattcctgg cttatgaacc tctactgcta aatatccact ctctggattt tcaaaggcaa
     2521 tctgagagct agcctcaccc atcccgcttc caatctcaca gacaatctct ttatatctat
     2581 cctttggaaa tatcttggat agttcaatcg ggcgatctaa cggaatttca tactcaccta
     2641 aatgctcgtc aatagctcca gcctgggcct tagtcattcg ctcaccgcgc aggcgaaagc
     2701 ttcttaagcg aaactcctca gatttttggc tagccatagt ggcaaagagt gccatctctg
     2761 cttggatacg cgcatgcctg gaaataacat atcgcgcgct gaagccgctg aacgctcctc
     2821 tcacctatct attaagcact atgaggtctt acttgatgta agcggggggg cagagagctt
     2881 catagccacc accaaagtca gctttgattg caacaaggtt ggttatgaga gctttatcga
     2941 tgcagttgga aagaggatca tctcggcgac ccttaatggt caaagcgttg atgtttcaaa
     3001 ttatgatggc gaaagtgttt ttctaaaaaa tcttcaagca agcaatgaat tagtaattga
     3061 aattgaggcg atttactcca agagcggtga gggtctgcag cgctcggttg atccagttga
     3121 taacgaggtc tatctctact ctcaaggtga gactgcattt ataagaaata tgtatccctg
     3181 ctttgatcaa ccagatctaa aagcaacttt tgcatttacg gcaatagctc cagctcattg
     3241 gcaggtgatt tcaaataatc cagtaaagca ggtaagtgat cttggggata aaaagaagtg
     3301 ggagttcact actaccccaa ttatgtcaac ctacattaca gctcttgttg caggtcctta
     3361 ctccttcatt catgataagt atgaaggcaa gaagagtatt ccgcttggaa tttactgtcg
     3421 caaatctcta tttgaacatc ttgattctga tgagatattc ctaattacta aacaaggctt
     3481 tgcctacttt gaagaggtct ttggtctggc atatccattt gataaatacg atcagattgc
     3541 tgtggtggac tttaactggg gcgctatgga aaatgctggc gctgttactt ttagagagga
     3601 cctcttagta tttcgctcca aagtaacaga gcggatgtat aacgcaagag ccaacacaat
     3661 ccttcatgag atggcccata tgtggtttgg agatatggtg actatgaagt ggtgggatga
     3721 tctatggctt aatgaatcat ttgcggagtg gtcttcatat ctagccctgg ttgaagcaac
     3781 taaattcaaa aattcttggg ccggctttaa cgctgagaga aagaattggg cctatcgcca
     3841 ggatcaactc tcatcaaccc atccgattgc taccgatatg gtcgatatcg acacggtaaa
     3901 agctaacttt gatggcatta cctatgccaa gggagcatca gttcttcatc aattagtggc
     3961 ccacgttggt cgagataact tcattaaggg cttgcagaag tactttgcta agcatgcttt
     4021 taaaaatact gtcttatcag atttattagt ggagcttgag gcaactagcg ggcgagattt
     4081 aagtagttgg gtatcaacct ggctacagac ttcaggggta aatactctgc gcccagtaat
     4141 tgaaaccgat ggcgagagct ataaatcaat tgctatcaaa caagaggctc caaagattcc
     4201 tgccggatca actgagcttc gtccgcatcg cgcagctgtt ggactatatg acttaaaaga
     4261 cggccaatta cttcgcagaa aatcagtgga gttagatatc tcaggtgatt taacacaagt
     4321 taaagagtta agtggtgaga agttggcaga tcttctgttg ataaatgatc atgaccttac
     4381 ctatgcaaag attcgctttg atgctagatc tattgctacc ttaaaagctc accttggtaa
     4441 attagatgat ggtctctctc gcgctctttg ttggtctgca gcttgggata tgtttagaga
     4501 tgctgagcta tctgcaactg actttataga aattgcatta acaggccttg ctggtgaaga
     4561 tgacataacc acagttacag ccttaatgac gcagttaaac accgcagttg agatctatgc
     4621 ctcagataag aatcgcgact ccttaagaga gaaggtggca ggtgcgcttt tatctcttct
     4681 taagtcagca aaggctggaa gtgatcacca gctccagttt gctagaagct ttgcagccct
     4741 ggcacatagc aaagagcagg gcgctgaaat tagaaatatt ctcgatggca aattccagga
     4801 tctaaagatt gatgctgatt taagatggca tttaattctc tgtctagtgg agcgaggatt
     4861 aagcacaaga gctgagattg atgcagagct aaagcgcgat aacaccctta ctggcgccct
     4921 tagccatgag agatgtattg cagcctttcc aactcttgag gcaaaagagg cagcatttaa
     4981 aaaggccacc gaggatgaga gcatcacaaa ttggtccaga ctctcagcta ttcaaggcta
     5041 taaccgcccg attcagcgcc acctgcatga gggatttgtt gatcgctact ttgatttaat
     5101 tcttgaaacc tacaacacca agagttatga agtctcaact agcatcattg atcttctcta
     5161 tcccagctat ttgatttcaa gtcagacact tgcaaaaaca gatgcctggc taaatggccc
     5221 tggaaaagat agtcatccaa cgcttcgccg ccatatcttg gaggctaagg attctttgga
     5281 gcgagcgcta agagttagag gaatagataa ctaattactc gccaatgcga gtaattacat
     5341 cacttggggc gcgattagct ttatttttct taacattctc aagtgcaact accgctaccc
     5401 agataaatag cgttatcgaa actggagtaa caaagaacca gagaaagccc tcaagaaaag
     5461 cgagcggctc accaggcatg gagccatctt catttaaagc taattgaatt gacatagatt
     5521 caagtttagg cagaagcgcc aacaactcca aatggagcca gatatctcac ccaacgctcc
     5581 tcagctcttg ctgttcctaa aatcctccaa gcagctccga ctggctcacg aggaattgag
     5641 cgaagtctcc aacctagctc tttcaaagtt ctatcagctt ttacatgatt gcagcgatga
     5701 caggcgctta caacattttc ccaagagtga tcgcctcccc tacttcgagg cagaacatga
     5761 tctattgatg ttgcagcgct ctcgcagtaa acacagcgac caccatcgcg agcaaatatt
     5821 gctctgcgtg agagtggaac agtgtggcga tatggaatct ttacaaattt atggagtcta
     5881 attactgcag gaagttccat tgatccagat gagtaatgaa gtgttacgcc agattcttca
     5941 atcatggtgg cgcggttatt taaaattaaa atgagcgcgc gtctatcaga aacgacacct
     6001 agtggctcgt aggtagcgtt tagaactaaa gtccttctcg atgagctatt caccgttcta
     6061 attcccccgt ctatccgacg cgtggctcgc gtcgtggggg aaatcctgcc cgaagttggt
     6121 gggttttgtg cggatatttg gttaagttct tataaagtct tgcccaatgg agctagatct
     6181 gagcctaaag caggtgctgg agtggtttct cggctccccc ttgcgaataa cgatcatctt
     6241 ggtcctggcg attttcattc agaaaatatc ctcaagggcc atcactaaaa ccttaagcaa
     6301 agtggcagag gctgatttca ttcctggtga gaagagatcg gtaacaagac aacgagagcg
     6361 agcaaagact gcagcctctg ttctaaatag ttcatcaaag gcagtcattg ggctaattgc
     6421 tggcgcaatg atccttggtg aattgggagt agatcttggt ccccttgtcg catctgcggg
     6481 cgtaattggt tttgccgttg gtcttggcgc tcagagcatt gtccgtgatg tcttctctgg
     6541 aatcataatg cttcttgaag atcaatacgg tgtaggcgat agcgttgagg ttttagaggt
     6601 aaaaggaaga gtagagagcg ttggccttag agtaacaact gttagagatc gaagaggaac
     6661 gctttggtat ttaagaaatg gcgagatatt aaaagttgga aatagttctc aaaaatctcg
     6721 ttcttgattg accatcgaat tagcagccat taccaaataa ctccagagct gctctcgcat
     6781 ctcattatcc atctcaagtg aatcaactgc tgatttcata tgagagagcc agacatccct
     6841 ctccttctct ccaattttaa attgcgcatg tcgcatgcga agtctgggat gtcctcttaa
     6901 ctggctatag gtatttggcc ctccccaata ctgctcaaga aacattaaca atcgctccgc
     6961 tgctcctttg aaatcatcat caggatacat cggacgaagt attggatctg tggcaacgag
     7021 ggcataaaag tgtgagacta aattctcaaa ggtttttctt ccgcccatct gctcataaag
     7081 actcataaaa agtactatct ggctttaatt tttagcgacc aaaggccaag aagtgctacc
     7141 ggaattgctg ataataaaca gagccagcca taagaaaggg ttgcgataac aacacctgca
     7201 attgctccac cacttgctcc agagagattc atgactagat cgctagcgcc ctgcgatgcg
     7261 cttttaaact ctggtgatac agattcagtt aataacgcag agccagcaat tagcgtgcag
     7321 gaccaaccaa ggccaagaag aaataatcca attcctagag ttattgcatc atctgctcta
     7381 gcaaatcctg atattagagc tgaagataga agtattgcta cgccaatttg aatgactctt
     7441 aatcttccaa ggcgatcagt taaggctccc acaagtggtg agaaggcata catgccaaga
     7501 acgtgaacgc taattaccaa accaattatg gttaacgtta catcaacatg ggccatatga
     7561 acgggcgtca tcaccattac tgcaaccatt gcaatgtggc caatagcaat tgaggcaata
     7621 gcaaatgagg ctctggcatt ggagcgaata tgcttgaggg caacctttgt cttgactctc
     7681 ggaagagttg ctgctcctga atcctttgct gcagttagat aaggatctgg ccttagaaat
     7741 aactggatga caagaacagc aagagctaga gtcacagctg aaatcaaata cggaccagta
     7801 agtcttggaa gtccaaatga ttctgctaaa ttcccagaag gctccattaa atttggtcca
     7861 gtaacggcgc caatagttga tccccaaacc acaaatgaga gttgctttgc tcgtttttca
     7921 tttgtagcaa gatcgattgc ggcaaatctt gcttgataac cagcagcaga agctgctcca
     7981 actagaaatg ttccagtaag cattaaatat atgttctctc ttgagccacc aaagattgca
     8041 agaagtgagc caatcacgcc agcaattagg ccaactgaga gagcaaggcg tcttcctccc
     8101 ctgcttgtga ggcgggcaag tggcagggca agagctgcag ctccgagtac tgatgaagtc
     8161 tgagcaaggc cggccagagt ctctgaatta gttattgatg caacaagaag ggagcctgct
     8221 gcaacagttc cagcaacgcc cactccattt aaaacttgtg cagttgctag aacctttaca
     8281 gttttttctt gcagggcagt attgctactc acttgcttac tcttaaccag actgcagtat
     8341 cagttggtag aaaatttcca tttagtggcg cacttgaatg aaggacttga gtattttctg
     8401 gtagctctat ctcgcttcca aagttaatgt agcaagcaaa gtcttcacct cttctaaatg
     8461 caacgacttg ttttggcgta tcaatccacg ccatggtccc atcaccaagt ccaggttcat
     8521 tctttctaat tgctagtgct tttcgataaa catttagtgt ggagtttgga tcattttctt
     8581 gtgattcggc agagtatctt ccccaccaag tacttggtgg aagccaagcc tgctccggag
     8641 ttaaattctc atttgatgaa aacccaaatg cgccgccact tgagctcttc catggaatag
     8701 ggactctgca gccatctctt cctcgatcct tgtagttgct catcttccat cttggatcag
     8761 ttaatcgatc ctctggaata tcccttactt caggtagacc tagctcttca ccttgataga
     8821 gatatgttcc accaggaaga gcaagagcca tcagggttgc actctttgct cgaagagttc
     8881 ctcttgcaat atctagcttg ttcacatcgc catggcgaga aaaagtggta tcaccatgat
     8941 ttgttaaacc taaatcaagg cgatcaacgg agcgaaccac atcatgattg ttaaataccc
     9001 aggaagttgg cgctccaact tcagccagag cgtcaatgga attattgatc tttttcctta
     9061 tttcatcggc tttccagagg gtggtgagca tctcaaagtt aaaggagttt tgtagttcat
     9121 cacttcttac atactttgca attcttgaag ccgggcttac ccaagcctct gccaccgcca
     9181 ttcgatcacc atcatatgaa tccaagatct ttctccagct gcgataaata tcgtgaaccc
     9241 cttcttgatc ccagaatggc ttatgttcgg ggcttaacat tcgagtgagg ttattcttgc
     9301 caaatatctt ttctatccaa ctacttcgaa catcaggaag gccactttct ttaaacatgc
     9361 catgagccac atcaatacga aatccatcaa cgcccttatc aagccagaac tttaaaacat
     9421 cttcaaagtg ttttactact tcattatttt cccaattcaa atctggttgc tcaacagcaa
     9481 agagatgtag ataccactgt ccttgcttgc catcagattc agtaactctc ttccaggcag
     9541 ggccgccaaa gactgcctgc caattatttg gaggtagttc cccgttttta cccttacctt
     9601 ctctaaaaat atatctatct cttgcagctg atcctggttt ggcagcaagc gcctctttaa
     9661 accacttatg ttgatccgag gtgtgatttg gaacgatatc gataattact ttaatgccta
     9721 gatcatgggc ttcttttatt aatagctcgc cctcttttaa agttccataa tcgggttcaa
     9781 tatctaaata atcagcaacg tcatagccat gatccttttg cggtgatgga taccaagggg
     9841 taatccagat tgcatctacg ccaagctttt ttagatatcc cagctttgat ctaatcccgc
     9901 caacatctcc aattccatca ccatttgaat cagcaaagga gcggatataa atctgatagg
     9961 taacagcatc cctccaccaa gggcgctggc ctctgggagc aagttttgaa ctcaactctt
    10021 cacctctaga tatttctcaa gaaaagctcg ctcacttgcc tcaagtgggc gagatttacc
    10081 gctctgctta tcaattccta cctgaaccga cttaatccga gcataaattg tgtcaccact
    10141 tttaatctca tagcccatta caaatgatga gttgccgatg gattccaccc agagctcaac
    10201 atctacaaag aatccacctt gcaagattgg agcaataaaa tctatctcag ctcttgcaac
    10261 caccatctct aaaattcctg gaacttcgcc tttgctgaca tgagaatann nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ctttggatca attccagatc ttgattgatc
    10441 aacatatgaa acctttaccg tctcaccgac tttaatactt cctgaatctg gtgtttcaag
    10501 tcctaggatc atcttaaata acgtggtttt tccagcgcca tttggcccaa taactccgac
    10561 aattccattt cgtggcagtg agaaggagag atcatcaatt aggaggcgat caccaaaacc
    10621 ttttgaaaga ttctcgacat caacaacaat atttccaagc cttggcccca tagggatttg
    10681 aatctcttca aaatcaagct ttctagcctt ctcagcctct gctgccatct cttcatatct
    10741 tgcaagacgt gactttgatt taacttggcg ccctttagca ttctgtctaa cccaatcaag
    10801 ctcctcagcc aatcgctttg cccgcttggc atccttttgt ccctcaactt ttaacctggc
    10861 tgcctttgtt tcaagatatg tcgagtagtt tccttcataa ggataggcgc gtccacgatc
    10921 taactccaag atccattgcg cgacgttatc taggaagtag cgatcatggg tgattgcaac
    10981 aactgttcct tcatactttg aaagaaattg ctcaagccat aacacagatt cggcatcaag
    11041 atggtttgtt ggctcatcaa gtagaagcag atctggcttt tgcaatagca acttacatag
    11101 cgcaactctt cttctctcac cgcctgataa aactttaaca tcagcatcag ggggcggaca
    11161 tctaagagcg tccattgctt gctctaactg tgaatctaaa tcccaagcac ttaagtgatc
    11221 aagcttttct tgcaagcttc ccatctctgc tagcaacttg tcataatcag catcagggct
    11281 tgccatctcc tcagatactt tgttgaagcg atcaaggagg gcaatagttt cggccactcc
    11341 ctcttggaca ttttccaaaa catttttatc ttcactaagc ggcggctctt gtagcaaaat
    11401 ccccacgcta tatccagggc ttaaataagc ttcaccattt gatggttgct gcagcccggc
    11461 cataatctgc aagaccgttg attttccagc gccatttggt ccaagaacgc cgatttttgc
    11521 cccgggcaga aacatcaaag tcacatcatc aagaatgacc ttatcgccat gcgctttacg
    11581 cgccttcttc attgtgtaaa tgaactctgc cacgaggcga a
//
LOCUS       DAOROD010000027        10370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_355_length_10370_cov_37.842269, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000027 DAOROD010000000
VERSION     DAOROD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_10370_cov_37.842269"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLW53_05340"
     CDS             <1..280
                     /locus_tag="PLW53_05340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD36878.1"
                     /translation="LNRFEIYFDGELAGFAEFKIANQKISYTHTEIDPKFGGKGLGSQ
                     LIKEALDEALEQNLEVAPYCSFVSAYIKKNSEKYLHLVPEGQRAKFDL"
     gene            complement(275..1171)
                     /gene="ccsB"
                     /locus_tag="PLW53_05345"
     CDS             complement(275..1171)
                     /gene="ccsB"
                     /locus_tag="PLW53_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRD36879.1"
                     /translation="MSQSDWAYLSNDLIYAALALYALSFLSYTYETAFSLRANKISGD
                     KHLDRSITTKSRKVASIVFLLATASLTLAVIARGISAERIPLGNMYEYSITGAMTFAL
                     AYLIIGLKYDLSWLGLPSSILILLILGTAITLLYRPSAPLVPALQSSWLVIHVSSAIL
                     SGGIFLLSNTVAGAYLWLDRIERKGERGEFAKRLPSLETLDQLSYRLVAFVFPLWTFA
                     VISGAIWAEAAWGRYWGWDPKETWAFITWILYASYLHARVTAGWKGRKAAWLCLIAGS
                     SYLFNYVYINVWGSGRHTYSGL"
     gene            complement(1168..2619)
                     /locus_tag="PLW53_05350"
     CDS             complement(1168..2619)
                     /locus_tag="PLW53_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRD36880.1"
                     /translation="MSAPELGTTSLLRYLWRQLTSMRTALILLLLLGVASIPGSIFPQ
                     RTQSPLKVREYFDDDPNGAKWLDRFYLFDVYGSPWFSAIYLLLFISLVGCVLPRSFHY
                     FKEIFKAPPAAPSILNTMESFQVSPASLERAEAWLKKNRFRISKTSDSISAEKGYLRE
                     TGNLLFHLSLIVVLLGIAGSSVFGMRGEAIVNVGERFINTPTNYDNLTPGRFFSLESL
                     PTFTIRVNNFSATYDRKTSQALDYELKVTTKDSLDSSEVNQIVKVNKPLTFGSTRVYL
                     QANGYSPLVTVRDSAGIVKFEGPVPFLPQDSNLSSIGAIKVPDMEPQIGFVSSFFPTA
                     ARDEVRGGFSSFPELLDPRLLVSVWKGDLKMDDGVPQSIYRIDTTDMERIGLWALSIG
                     ESYSFEVGSITLNGVVPWVNLQVVRDPGKQYALIGSILAIVGLLISLFIRQRRIWVRE
                     VGGSLEIAGLALNKLPGLEDEIGKMIQELGDQK"
     gene            complement(2619..3344)
                     /locus_tag="PLW53_05355"
     CDS             complement(2619..3344)
                     /locus_tag="PLW53_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRD36881.1"
                     /translation="MSDFLVNQVFDGSLLAASLVALIAGLISFASPCVLPLLPGYLGY
                     VSGAFASKGRVLLGSFLFVLGFAFLFMTYGVAFGALGDAILSSSSILARLLGVLTIFF
                     GLIFLFSEKFYRSFKFPFVASTGLISAPILGFMFGLGWTPCIGPTLGAVQTLALIEAS
                     ATRGAILSLFYCIGLGAPFIFFALFLERSNKFRRYLIQRGQVISIIGGLFLISIGLLQ
                     VFGLWEDLMASLRGTISSFIPVI"
     gene            complement(3337..3876)
                     /locus_tag="PLW53_05360"
     CDS             complement(3337..3876)
                     /locus_tag="PLW53_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRD36882.1"
                     /translation="MRKLIILLCLPLLIACSSNGVTSGDEQAFISGNGVATYISPDKR
                     KVAPEISGPTLSGGSFQAEPGKLLILNVWASWCSPCRAEAGALQELSVAYPEVQFLGV
                     LTRDTRVAAQSFVDRFGITYPSLTDDAILLQFHGQLIPNAIPTTLIIDSQSRVAARIS
                     GEVTYSSLKELIERVKSDE"
     gene            complement(3873..4514)
                     /locus_tag="PLW53_05365"
     CDS             complement(3873..4514)
                     /locus_tag="PLW53_05365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD36883.1"
                     /translation="MSDEAAISQSVHLLRHGQVENPKNILYGRQPGWKLSERGHEMAK
                     TVATWSKELSLGAIHASPLERAQQTAAPIAAQHGLTIKTDENLIEAENIFEGKPFDVS
                     GAIKRPATWRHLWNPWRPSWGEPYKVQVSRMSKAVNDARLAADGKDALLVSHQLPIWI
                     MRLSIEGRSFLHDPRKRQCTLASVTSIHFDKSGKVLGLTYSEPAAHLLPDNKK"
     gene            4568..5329
                     /locus_tag="PLW53_05370"
     CDS             4568..5329
                     /locus_tag="PLW53_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRD36884.1"
                     /translation="MPRIAFFDVDNTITRGSTLYFLGKGMYNRGFFTKRDIGAWVLAN
                     IRFRMTGTEKSEVISRFQKAATDFIGGHDVKEIRIIGEQIYSEFVSPSIWEGTIELAK
                     EHLSSGDEVWLVTASPEDFANLIAERLGFTGAIGTKAEVKDGKYTGNLNGKLLHGKEK
                     AIAITELTKERGIDLKDCFAYSDSHNDLPLLSAVGHPRAINPDAKLRIIAFAQSWPVH
                     DFRRLRWLNRFIGPFISRLAGFWVFCTSGIRRSSK"
     gene            5378..7579
                     /locus_tag="PLW53_05375"
     CDS             5378..7579
                     /locus_tag="PLW53_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRD36885.1"
                     /translation="MSLTGVVNSSSGFSPTFAAELRERSDEDLEKLFTLRPDLITPIP
                     ADMSSLATRACSAPSLLRALETLNQWQFQVLEVCAALRDPFTIKEVITLSDKASQEVI
                     LHLRSLALIYSDGAGFRLPRAVRDLLGNEPAGLGPESATKIDLKLLDKAPAGAKAVLE
                     KLTWGPPRGQVGDIRKKGTPINWLLENNLLIPIDTSTVALAREVGIYLRGNKVHKELL
                     PTEPKITGVATKDVDTQRAALASISNTLRWVAELLNFWSEESPSARQSGGLGVRDLKK
                     AADHLGVEESCAAFIAEIAYLSGLINLEADGQIIPTTLFDLWQNKDPEAQWSELVSLW
                     RVTSRVAGLVGRSESRNLTALSSELDRSNASLIRNLTLDLLLKNPGISANPESVKAAV
                     LWRYPHRRGISITSELVQWSLREAEWLGITGGGALSQYGMSFLKDEEDLGINGALPKP
                     VEHILVQADNTAIAPGPLTIEVARMLSTFADIESRGGATVYRFSESSIRRGLDHGHSG
                     EEIRAFLNKVSKSAIPQPLEYLIGDVAKKHGKLRVGYANTYIRCEDQSLIAAITSDKK
                     LSHITFRQIAPEILICDSESGEVMEELRGAGYFPAGENAKGSVINMPIANRSKSRPKP
                     PRVIGELSKPSSAILSVAIRTLRTGERAAEQRPVGQIPRTTANETMDLLNEYLGKGVS
                     LRIGYADTNGGVSLRIIDPLSISLGTLVARDHATNAITPFKIARITGVTTA"
     gene            7605..8675
                     /locus_tag="PLW53_05380"
     CDS             7605..8675
                     /locus_tag="PLW53_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD36886.1"
                     /translation="MRDQMLKDLQALVECESPSSDLAACAKVLEVANQITAKVVGTSA
                     EIIQESGRPVYWLGSKNPEVVLLTHLDTVWPIGSFAPLWRVDGDIASGPGVFDMKSGF
                     IQALYAMRGQDLDRVALIATTDEETGSATSRKLIEEVSKKAKAVLVMEASLDGKLKIG
                     RKGTSMYQITIHGRAAHAGLEPEKGINATVEISKIVSKLIALENKDLGTSVVPTVMTS
                     GSTTNTVPALASLDVDSRSFTMAEMKRVEEAIRALKPENKDARIEITGGINRPPMETS
                     ATSELYALAEKVAVKLAMAPLGSASVGGASDGNFAAIHTKVLDGLGSLGGGAHANNEH
                     VLVSHLEPRSNLIAALVKEILL"
     gene            8672..10315
                     /locus_tag="PLW53_05385"
     CDS             8672..10315
                     /locus_tag="PLW53_05385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF027528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD36887.1"
                     /translation="MTQGPLIVQSDKTLLLDIDHPLSAECRQAIAPFAELEKSPEHIH
                     TYRLTSLGLWNARASGHDAEQVIDTLLKYSRYAVAHALLLDIAEVMGRYGRLRIESHP
                     VHGLVLISTDVAVLTEVMRAKKVAPLLGTKVDDETVTVHPSQRGHLKQALLRLGWPAE
                     DFAGYVDGQAHAISLNEDGWKLREYQKLAAEGFWHGGSGVVVLPCGAGKTLVGAAAMA
                     HAQATTLILVTNTVAARQWRDELIKRTSLNEDEIGEYSGAKKEIRPVTIATYQVMTTR
                     KKGLYAHLDLFDSHDWGLIIYDEVHLLPAPIFRFTADIQSRRRLGLTATLVREDGMEG
                     EVFSLIGPKRFDVPWKEIEAQGYIAPADCVEVRVTLTDHERLTYATAEQEDKYRTCAT
                     TATKKNVVIALAKQHEDDQVLIIGQYISQIDEIAAELAVPIIKGDTPVKEREELYGKF
                     RSGEIKCLVVSKVANFSIDLPEASIAIQVSGTFGSRQEEAQRLGRILRPKSDGRSARF
                     YSLVARDTIDQDFAQNRQRFLAEQGYSYRIIDADEVLPR"
BASE COUNT         3145 a         2152 c         2296 g         2777 t
ORIGIN      
        1 gctcaataga tttgaaatct attttgatgg agagctagca ggctttgcag agtttaagat
       61 tgctaatcaa aagatttcct atacccatac agaaattgat cctaaatttg gtggtaaagg
      121 gttgggcagt caactaataa aagaagcttt agatgaagca cttgagcaaa atcttgaggt
      181 tgcgccttac tgttcttttg tctctgccta tattaagaaa aatagcgaga agtatcttca
      241 cttagtccca gagggccagc gagcaaagtt tgatctatag accgctatag gtatgtcttc
      301 cgcttcccca tacattgata taaacataat taaatagata agaagatcca gcaattaggc
      361 agagccaagc tgcctttctt cccttccatc ctgcggtcac tcgcgcatgt aaatatgagg
      421 catagagaat ccaggtaata aatgcccaag tctcttttgg atcccatccc caatagcgac
      481 cccaagctgc ctctgcccag atagcgccag agataactgc aaaggtccat agcggaaata
      541 caaatgctac taagcgatat gaaagctgat ctaaggtctc aagagatgga agtctttttg
      601 caaactcacc tctctcgcct tttctctcaa tacgatctaa ccagagataa gcgccagcta
      661 ctgtatttga aagcaaaaag attccgccag agagaattgc tgaagatacg tgaatcacta
      721 accagctaga ttgcagcgcg ggaactagtg gagcacttgg tctatataaa agagtaatag
      781 ctgttccaag gattaaaaga atcagaattg aactaggtag accaagccaa cttaagtcat
      841 atttaagccc aattattaaa tatgccaatg caaatgtcat tgcgcctgtt attgaatatt
      901 catacatatt gccaagtggt attcgctcag ctgaaattcc acgcgcaatt accgccagag
      961 ttagggatgc tgttgctaat aagaatacga tgctggctac ttttcttgat ttagtggtaa
     1021 tgcttcgatc taaatgttta tcacctgaaa ttttatttgc acgtagtgaa aatgcagttt
     1081 cataggtgta ggagagaaat gaaagcgcat agagagcaag agcggcataa attagatcgt
     1141 ttgatagata tgcccaatcg ctctgactca cttttgatca cctaactctt gaatcatctt
     1201 acctatctca tcttctagcc ctggtagctt attaagagca agccctgcga tttctaaact
     1261 gcccccaact tctctaaccc atatccgtct ctgacgaata aagagtgaga taagaagacc
     1321 aacaatggcg agaatacttc caattagagc gtattgcttg ccgggatctc taaccacttg
     1381 aagatttacc caaggaacaa ctccattaag tgtgattgac cccacctcaa aggagtaact
     1441 ctcaccgatg cttagcgccc agagtccaat tcgctccata tctgtagtgt cgattcgata
     1501 gatggattgt ggaacgccat catccatctt taaatctccc ttccaaactg aaactagaag
     1561 ccttggatca agtagctcag gaaatgaaga gaagccaccg cgaacttcat ctcttgcagc
     1621 agttgggaaa aaagagctta caaaaccaat ctgtggctcc atatcaggaa ccttgattgc
     1681 acctattgaa cttaagtttg aatcttgggg aagaaatgga actgggcctt caaatttaac
     1741 aatacctgcg ctatctctaa ctgtaacaag aggtgagtaa ccattagctt gaagataaac
     1801 tcttgttgag ccaaaggtga gcggcttatt taccttgact atctgattaa cttcagatga
     1861 atccagagaa tctttagtgg tgacttttaa ctcataatct aaggcttgag aagttttacg
     1921 gtcataagtg gctgaaaaat tatttactct aatagtaaaa gttggaagag attcaagaga
     1981 aaagaaccgt cctggggtga gattgtcata atttgttgga gtattaataa atcgctcgcc
     2041 aacattgaca atcgcctcac ctctcattcc aaatactgaa cttccagcaa tgcctagtag
     2101 aactacaatt aaagatagat ggaagagtaa attgccagtc tctcttaaat accctttctc
     2161 tgctgatatt gaatcacttg tttttgaaat cctaaatcta tttttcttta accatgcttc
     2221 agctctttct agacttgctg ggcttacttg aaaactctcc attgtgttta gtattgatgg
     2281 agcagcaggg ggagctttaa atatctcttt aaagtaatga aaagatctag gtaatacgca
     2341 accaactaga gagataaaaa gaagaagata gatagcagag aaccagggag agccatagac
     2401 atcaaataga tagaagcgat ctagccactt agctccgttt ggatcatcat caaagtattc
     2461 acgaactttt agtgggctct gtgttcgctg cggaaatatc gatccaggaa ttgatgcaac
     2521 accaagtaat agcagcaaaa tcagagctgt gcgcatactt gttaattgac gccatagata
     2581 gcgaagaagt gatgtagttc ctagctcggg cgcactcatt aaatcaccgg aataaatgag
     2641 ctaatagtgc cgcgtagtga tgccatcaaa tcttcccaga gaccaaatac ttggagcaga
     2701 ccaatggata taagaaatag gccaccgata attgaaatta cctgacctct ttggattaga
     2761 taacgacgaa acttatttga gcgctctaaa aagagtgcaa agaaaataaa aggcgcacca
     2821 agtcctatgc aataaaagag agaaagaatc gcgccacgtg ttgcgctagc ttcgatcaaa
     2881 gctagagtct gcacagcgcc aagtgttggg ccgatacatg gtgtccagcc aaggccaaac
     2941 ataaagccga gaatcggagc acttattagt cctgttgagg caacaaaagg gaatttaaag
     3001 gatctataga acttctctga aaatagaaag attaatccaa agaagatcgt taagactcca
     3061 agaaggcgcg caagaataga tgaactactt aatatcgcat cacctagagc gccaaaagcc
     3121 acaccatagg tcataaataa aaatgcgaat ccaagaacaa ataagaatga acctagaaga
     3181 acgcgccctt tagatgcaaa agctcctgat acataaccca aataaccagg aagtagtggc
     3241 aatacacaag gcgaagcaaa agaaattaaa cctgcaataa gtgcaactaa gctggcagca
     3301 agaaggctgc cgtcaaatac ctgattaact agaaaatcac tcatcactct ttactctttc
     3361 aattaactcc ttaagtgagg aataagtaac ttcgccgcta attcttgctg caactctaga
     3421 ttgactatca attattaggg ttgttggaat ggcatttgga attaactgtc catgaaactg
     3481 aagcaaaata gcatcatcag ttaatgatgg gtaggtaatg ccaaagcgat caacaaatga
     3541 ttgcgcagct actcgagtat ccctcgttag aactcctagg aattgaacct ctggataagc
     3601 gacagagagt tcttgcaagg ctcctgcctc tgccctacag ggagagcacc acgaggccca
     3661 gacattgaga atcaatagct ttcctggctc tgcttgaaag cttccaccac ttaaagtggg
     3721 gccagaaatt tctggagcta cttttctctt atcaggtgag atatatgtag ctacaccatt
     3781 tcctgagata aatgcctgtt catcaccgct agtaactcca ttgctcgaac atgctattag
     3841 taacggcagg caaagaagaa ttattagttt tctcattttt tattatctgg aagtagatgc
     3901 gcagcaggtt cactataggt aagacctaac acttttccgc tcttatcaaa atgaatgcta
     3961 gtaactgagg caagtgtgca ttggcgtttt cttggatcat ggagaaaaga tcttccttca
     4021 attgatagac gcataatcca aatcggcaat tgatgtgaaa caagtaaagc atctttccca
     4081 tcagctgcta ggcgagcatc gttaacagcc ttactcattc gagatacttg cactttataa
     4141 ggctcgcccc aggaagggcg ccaaggattc cagagatggc gccaggtagc tgggcgttta
     4201 atcgcgcctg aaacatcaaa tggttttcct tcaaagatat tttcagcttc aatcaaattc
     4261 tcatctgtct taattgttaa gccatgttgt gctgcaatcg gcgcagcagt ttgctgtgct
     4321 cgctcaagtg gcgatgcatg aattgctcca agtgagagct ctttactcca ggtcgctaca
     4381 gtcttagcca tttcatggcc tcgctctgaa agtttccagc ctggctggcg gccatagaga
     4441 atgttttttg ggttctcaac ttggccatgg cgcaggagat gaacactctg tgaaattgct
     4501 gcctcatcgc tcatagcctt aagcataaag gcagaagtaa tactgcgctt ttcgctactc
     4561 tgacttcatg ccacggatag ctttctttga tgtagataac acaatcaccc gtggatcgac
     4621 cctctatttt ctaggtaagg gtatgtataa ccgtggtttt tttaccaagc gagatatcgg
     4681 ggcatgggtt cttgccaaca ttcgcttccg aatgactggc actgaaaagt cagaagttat
     4741 tagccgcttt caaaaggctg caacagattt cattggtggc catgatgtta aagagatccg
     4801 aataattgga gagcagatct acagtgaatt tgtttcacca tctatttggg aaggaacaat
     4861 tgagcttgca aaagagcatt taagttctgg agatgaagtc tggttagtaa cagcctcccc
     4921 tgaagacttt gcaaacttaa ttgcagagcg ccttggtttc accggagcaa ttggaacaaa
     4981 agctgaagtg aaagatggta aatacactgg aaatctcaat ggaaaacttc ttcatggcaa
     5041 ggagaaagca attgcgatta ctgaactaac aaaagaacga ggaatagatc ttaaagattg
     5101 ttttgcatac tctgattctc ataatgacct gccacttcta agcgcagtgg gccatccaag
     5161 agctatcaat cctgatgcca aacttcgtat tattgcattt gcgcagagct ggccggtaca
     5221 tgattttaga aggctacgct ggttaaatcg tttcattggt ccttttatct ctcgtttagc
     5281 cggattctgg gttttttgta cctcgggaat tagaagatct tccaaataat cttccaggca
     5341 gacaaggact taacttcttc ggtaatgtag gccagttatg tcgctaacag gtgtagtaaa
     5401 tagttcctcg ggctttagcc ctacctttgc tgctgaactt agagagcgaa gcgatgaaga
     5461 tctagagaag ttatttactc tccgcccaga tttaattact ccgattcctg cagatatgag
     5521 ctctcttgcc actcgcgcct gctctgcacc cagcctgctt agagccctag aaacgctaaa
     5581 tcaatggcaa ttccaagtcc ttgaagtctg cgcagcccta agagatccat tcacaattaa
     5641 ggaagtaatc accctcagcg ataaggcaag tcaggaagta attcttcacc tgcgatcact
     5701 tgctcttatc tattcagatg gcgctggttt tagattgcca agagcagtaa gagatttgct
     5761 tggaaatgaa cctgctggtc ttggaccaga gtcagcgaca aagattgatt tgaagttact
     5821 agataaagcg ccagcaggag ctaaagcagt tttagagaaa ttaacttggg gcccgccaag
     5881 agggcaagtt ggagatatta gaaagaaagg aacgccaata aattggcttc ttgaaaataa
     5941 tcttttgatt cctattgata cctcaactgt agctcttgcc agagaggttg ggatttacct
     6001 tcgaggaaac aaagttcaca aagagttatt gccaacagag ccaaagataa ctggagttgc
     6061 taccaaagat gtagataccc agagagcagc gttagcctca atttcaaata ctctgcgctg
     6121 ggttgccgaa ctacttaatt tctggtcaga ggaatcacca agtgctcgcc aatcaggagg
     6181 gcttggagta agagatttaa agaaagctgc agatcactta ggagttgaag aaagttgtgc
     6241 tgcatttatt gcagagattg cttatctatc aggattaata aatctagagg cagatgggca
     6301 gatcattcct acaactctct ttgatctctg gcaaaacaaa gatccagagg ctcaatggag
     6361 cgagctagta agtctttgga gagtgacttc aagagttgct ggtcttgttg gcagaagtga
     6421 atctcgaaac ttaactgctt tgagtagtga acttgataga agtaatgcca gtttaattag
     6481 aaacctaacc ttggatttgc ttcttaaaaa ccctggaatc tcagctaacc cagagagtgt
     6541 taaggcagct gttctatggc gctatccaca ccggcgcggt atttcaataa cctcagagtt
     6601 ggtgcaatgg agccttcgag aagctgagtg gctgggaatt acaggcggcg gggcgctctc
     6661 tcaatatggg atgagttttt tgaaagatga agaggatttg ggaatcaatg gcgcgcttcc
     6721 aaaacctgtt gaacatattt tagttcaggc agataacaca gctattgctc cagggccgct
     6781 cacaatagaa gttgctcgaa tgctctcgac ctttgcagat attgaaagtc gaggcggcgc
     6841 aactgtctat cgctttagtg aaagttctat tcggcgcggc ctagatcatg ggcattcagg
     6901 tgaggagatt agagctttcc taaataaggt ttcaaagagc gcaattcctc aacctttgga
     6961 atatttgatt ggtgatgtcg caaagaaaca tggaaagctt agagttggtt atgccaatac
     7021 ctacattcgc tgtgaagatc agagtttaat tgcggcaatt acctctgata aaaaactctc
     7081 tcatataacg ttccgacaaa ttgctccaga gattttgata tgtgatagtg aaagcggaga
     7141 agttatggag gagttaagag gcgctggata ttttcctgcg ggagaaaatg ccaaaggaag
     7201 tgttattaat atgccaatag ctaatcgctc taaatcaaga cctaagccac ctagggttat
     7261 tggggagcta agcaagcctt catctgcgat attaagtgtt gctattagaa cgttaagaac
     7321 tggcgagcga gcagctgagc agcgcccggt tggccaaatc cctagaacaa cagccaatga
     7381 aacaatggat ttgcttaatg agtatctagg aaaaggcgtc tctcttcgta ttggctatgc
     7441 cgataccaat ggcggagtct cacttcgcat cattgacccg ctctcaatct ccctaggaac
     7501 cttggtggct agagaccatg caactaatgc cataacccct ttcaaaatag ctcgaattac
     7561 gggggtcact acagcatgag taacgattac ggcagacttc acctatgaga gatcagatgc
     7621 tcaaagacct gcaagccctc gttgagtgtg agagccctag tagtgatctt gctgcctgcg
     7681 caaaagtttt agaggttgca aaccaaatta cagcgaaggt agttggcaca agcgcagaga
     7741 taattcagga atcaggaaga ccggtctact ggcttggtag taaaaaccca gaagttgttc
     7801 tgctaactca tcttgatacc gtttggccaa ttggatcttt tgctccgctc tggagagtcg
     7861 atggagatat tgcatctggc cctggcgtat ttgatatgaa atcaggtttt atccaagctc
     7921 tctatgcaat gcgaggtcaa gatcttgacc gtgttgctct tattgcaaca acagatgagg
     7981 agactggctc tgctacttca cgcaaactaa ttgaggaagt aagtaagaaa gcaaaagcag
     8041 tattagtcat ggaagcatca cttgacggca agctaaaaat tggtcgcaaa ggaacttcta
     8101 tgtatcagat aacaattcat ggcagagcag cccatgctgg cttagagcca gaaaagggaa
     8161 ttaatgcaac tgttgaaatt tcaaagattg tcagcaaatt aattgctcta gagaataaag
     8221 atttaggaac cagcgttgtt ccaactgtta tgacctctgg aagcacaact aatactgttc
     8281 cagctcttgc aagccttgat gttgattcaa gatctttcac catggccgag atgaagcgag
     8341 tagaggaggc aattagagct ttaaaaccag agaataagga tgctcgaatt gaaattactg
     8401 gcggtattaa tcgaccacca atggaaacct ctgcaacaag tgagctctat gctcttgctg
     8461 aaaaggttgc cgtaaagcta gcaatggcgc ctcttggatc agcgagtgtt ggcggggcaa
     8521 gtgatggaaa ttttgcagca atacatacca aagtccttga tgggcttggc tcacttggcg
     8581 gcggggctca tgcaaataat gagcatgtat tggtctcaca ccttgagcct cgctcaaatt
     8641 taattgctgc actcgttaaa gaaattcttc tatgactcaa ggtcctttaa tcgttcaaag
     8701 cgataagact ctgcttctag atatcgatca tccactctct gcagaatgtc gccaagccat
     8761 agctcctttt gcagagcttg agaaatcacc tgaacacatt cacacctacc gtttaacctc
     8821 tcttggttta tggaatgcaa gggcatcggg acatgacgca gaacaagtaa ttgatacttt
     8881 gcttaaatac tcaagatatg ccgtagcgca tgctttatta cttgatattg cagaagttat
     8941 ggggcgatat ggcaggttaa gaattgaatc tcatccagta catggtctag ttcttatctc
     9001 aacggatgta gcggtattaa ctgaagttat gcgggcaaaa aaagtagccc cgcttcttgg
     9061 aacaaaggtt gatgatgaaa cagtaactgt tcacccaagt caaagaggcc atctaaaaca
     9121 agcccttctt cgtttaggtt ggccagctga ggattttgcg ggatatgtcg atggacaagc
     9181 acatgcaatc tcacttaatg aagatggttg gaaacttagg gagtatcaaa aacttgctgc
     9241 cgaaggtttc tggcatggtg gatctggcgt tgtagtactt ccttgtgggg caggaaaaac
     9301 tctagtggga gcagctgcta tggctcatgc tcaagccaca acgctgattc tggtgacaaa
     9361 tactgttgct gcaagacagt ggcgagatga gttaataaag cgcacatctc taaatgaaga
     9421 tgagattggc gaatactctg gagcaaagaa agagattcgc ccagtaacga ttgctaccta
     9481 tcaagtgatg accacaagaa agaagggtct ctacgctcat ttagacctct ttgattccca
     9541 tgattggggc ttaattattt atgacgaggt tcatctactc cccgccccta tctttagatt
     9601 tactgcagat attcaatctc gtcgccgttt aggccttacc gcaactctgg ttcgtgaaga
     9661 tgggatggag ggtgaggtct tctcacttat tggaccgaag agatttgatg ttccttggaa
     9721 agagattgaa gcgcaaggat atatcgcccc tgctgattgc gttgaggtaa gagtcacgct
     9781 taccgatcat gaacgcctaa cttatgccac agctgaacag gaagataagt atcgcacctg
     9841 cgcaactact gccacaaaga aaaatgtagt gattgcgcta gcgaagcaac atgaagatga
     9901 ccaagtttta attattggac aatacatttc acagattgat gagattgccg cagagcttgc
     9961 tgtgccaata attaaaggtg ataccccagt aaaagaacgt gaagagttat atggcaaatt
    10021 tcgaagtggc gagattaaat gtctcgtagt ttcaaaagtt gctaacttct caatcgattt
    10081 accagaggca tcaattgcaa tacaagtttc aggcaccttt ggttcaagac aagaagaggc
    10141 acagcgactt ggacgaatcc ttagacctaa atctgatgga aggagtgctc gcttttattc
    10201 ccttgtagct agagacacaa ttgatcaaga ctttgcccag aatagacaga ggttcttagc
    10261 tgaacaggga tactcctaca gaattattga tgctgacgaa gtattgcctc gctaaatcgc
    10321 tttttatcaa ctctaaagta atcgtgcttt gccccattta ttaacgttac
//
LOCUS       DAOROD010000028        10008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_375_length_10008_cov_32.965036, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000028 DAOROD010000000
VERSION     DAOROD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_10008_cov_32.965036"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLW53_05390"
     CDS             <1..273
                     /locus_tag="PLW53_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRD36888.1"
                     /translation="AILDTNCDPDEVDFKIPGNDDAIRSVALLTRVIADAVAAGLQAR
                     SANVAKEAMDAAKSAEGSVAAGEPLADWEKELLAGTPEAPTENQGA"
     gene            274..1101
                     /gene="tsf"
                     /locus_tag="PLW53_05395"
     CDS             274..1101
                     /gene="tsf"
                     /locus_tag="PLW53_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRD36889.1"
                     /translation="MSYSAADVKRLREATAAGMVDCKKALDEAEGDFDKAVEIIRVKG
                     LKGVTKREGRATSNGLIAAKSEGNTSVMLELNCETDFVAKGERFQAVANELLEHLFSS
                     KQSDLAAFLTSKLATGKAVQEVIDEANATMGEKVELRRLAVIQGSANAQYLHRTSPDL
                     PPQVGVLVALAKDDAVVGKDIAQHIAAFAPQYLDRASVPADVLEHERKVAEETAKNEG
                     KPEAAIAKITEGRVTGFIKEVALLEQAFAKDQKKSVAAILTEAQNSVSAFYRFRVGQ"
     gene            1144..1866
                     /gene="pyrH"
                     /locus_tag="PLW53_05400"
     CDS             1144..1866
                     /gene="pyrH"
                     /locus_tag="PLW53_05400"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRD36890.1"
                     /translation="MADKRKAYKRVLLKLSGEVFGGSKGVGVDPDVVHGVAEQIAEVV
                     RSGTQVAIVVGGGNYFRGAELQERGMDRARADYMGMLGTVMNCLALQDFLEKEGIETR
                     VQTAIAMGQVAEPYIPRRSIRHLEKGRVVIFGAGAGMPFFTTDTVAAQRALEIGVDAL
                     LLAKSGVDGVYSADPRKDKDAKRYDVITYDEVLSKSLAVADAAAFSLCRENKLPIVVF
                     NLLENGNIARAVRGEAIGTLVN"
     gene            1876..2436
                     /gene="frr"
                     /locus_tag="PLW53_05405"
     CDS             1876..2436
                     /gene="frr"
                     /locus_tag="PLW53_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRD36891.1"
                     /translation="MSIQTVIADTNTKMNKAVDVAKEDMAAIRTGRAHPSMFAKIMVE
                     YYGTLTPLSQLATIQVPEARTAMITPFDKGAITSIEKSIRESDLGVNPGGDGTVIRVN
                     FPQLTEERRKEFIKVAKTKAEDSKISIRSIRRAAKEAMEKLEKDGQIGKDDLSRGEKE
                     LEKVTSDHVAKIDEMLKHKEAELLEV"
     gene            2452..3297
                     /locus_tag="PLW53_05410"
     CDS             2452..3297
                     /locus_tag="PLW53_05410"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRD36892.1"
                     /translation="MSDLHSINEAINKKAGRKLFPSILVGLLLLAIVFSTMSYAPIAF
                     AAFVTVAVLIALKELTAAFKARGITSNYLGLALSTALILGSTWIGGLPGLTVSMVVSL
                     IFNLLLVLRGGVEGFVSRATASAFALLYPGFVAGFILLLARSGEGFSYIATLVIMVGC
                     NDTFAWAFGVLLGKHPLAPKISPKKTWEGFFGGLIFSAIGSSLAFYYLLDYHPVIGAL
                     AGCAGALTATVGDLLESGIKRDLSIKDMGTILPGHGGMLDRLDAVLITAPLLWCVLEI
                     LKRFL"
     gene            3294..4400
                     /gene="rlmN"
                     /locus_tag="PLW53_05415"
     CDS             3294..4400
                     /gene="rlmN"
                     /locus_tag="PLW53_05415"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRD36893.1"
                     /translation="MSEIKLVFDEVKPKKKAPEHLADLTIAERRAKAESLGLPAFRAN
                     QIATHYFTHYNEEPDSWSDIPAAMRPLVAEHFLPKLLTPIRQVECDKGKTRKDLWRLH
                     DGVLVETVLMRYKDRTTVCISSQAGCGMNCPFCATGQAGLTRNLTAGEITSQVVAAAR
                     ACASGELPGGPTRLSNVVFMGMGEPLANYNSVMKSVRNIITPQPDGLGIGARSVTVST
                     VGLVPGIEKLTSEDLQITLAVSLHTPDDELRDTLVPVNSRWKVKEVLSAADKYVAMTG
                     RRYSIEYALIRDINDQKWRADLLGRLLKNRHAHVNLIPLNPTPGSKWTASRKEDEIAF
                     VKTLEKYGVPVTVRDTRGREIDGACGQLAAASKP"
     gene            4485..5912
                     /locus_tag="PLW53_05420"
     CDS             4485..5912
                     /locus_tag="PLW53_05420"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HRD36894.1"
                     /translation="MKTLQNFINGKSVPSSSDKTQDLINPSTGQVFAKAPVSNAADID
                     KAMKSAESAFEIWKESTPGERQKAINKIADAIEARSEELIGIESENTGKPIAVTRAEE
                     IGPMLDQIRFFAGAARHLEGRSAAEYSKGHTSFIRREPIGVCAQVTPWNYPMMMAVWK
                     WAPAIAAGNTVVLKPSDTTPVSTLWMAELMQEFLPEGVINVVVGDRETGRHLVEHEIP
                     QFVSITGSVKAGMEVAASAAKDLKRIHLELGGKAPVVIFDDANIEAACEWIAVAGYFN
                     AGQDCTAATRVLVEASAYEDVVALLSEQAKNVIKVGMPDEDVLVGPVNNASQLARVKG
                     FIDRAPAHARITAGGSAIDRPGYFWNPTVVADLRQDDEMIQSEIFAPVITVQKFTDEA
                     EAVRYANGVKYGLASSVWTKDHGRAMRMAKAFDFGCVWINTHIPMVAEMPHGGFKHSG
                     HGKDLSGYGFEEYTRIKHVMTNLNA"
     gene            5961..7163
                     /locus_tag="PLW53_05425"
     CDS             5961..7163
                     /locus_tag="PLW53_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD36895.1"
                     /translation="MRKDPEKLSPEARSIIRAQVTRRSLLVGAGAIAGASTLAACGTG
                     STDSGAEGVVDVSDSEKIVRWANWPLYLDYNEDTQEYPTLKAFEAATGISATYTEDID
                     DNNTFYGKVQGQLGIGSDIGYDVVTPTDWMAARWIELGYAQELDAANIPNKSNIRAVL
                     ANVGFDPGRNYSLTWQSGFAGFGWNTQKIPKGVRTIEDLFAPANKGRIVVLSELRDTI
                     GIILQYQGVDPSSDFTEDQYMNAIDFLKGKVADGFIRKVRGNSYSEDLVNGDAVAVIG
                     WSGDMFILKSENDGKFDFAIPESGGTLWSDNMLVPSTSTHKKNAELLMNHYYDPAIAA
                     EVAAYVNYICPVEAAQSELEKIDPDLAASPFIFPDEATLAKVKVFRSLDADESTNFQA
                     AFDEAVGN"
     gene            7185..8312
                     /locus_tag="PLW53_05430"
     CDS             7185..8312
                     /locus_tag="PLW53_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD36896.1"
                     /translation="MAKTKVSQDTGDLVLNRISKYYGDFCAVDDLSLTIPDGSFFALL
                     GPSGCGKTTTLRMIAGLEEPTAGTISIGDTDITTQKSYRRNVNTVFQNYALFPHLSIF
                     ENIAFGLRRRGIKDVKDEVEKALNLVELGHLSERKPAQLSGGQQQRIAVARAIVNRPE
                     VLLLDEPLGALDLKLRRQMQIELKRIQLEVGITFIHVTHDQEEAMTMADTIAVMNQGK
                     IEQMGSATELYEAPKTAFVANFLGQCNLIKVKVQSRSGGKVVVSAKGHTFEIPESRVS
                     SSNDALLLGVRPEKLRLDDKGVNSIKECVVTDRSFVGTSTQYLVRTPWDLELVVFEQN
                     DEGLHDLRVGDKVSLEWDSEHTFGLDGAQDVNAGADLDDDE"
     gene            8316..9218
                     /locus_tag="PLW53_05435"
     CDS             8316..9218
                     /locus_tag="PLW53_05435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36897.1"
                     /translation="MAFLHGATKSKSGGGSALKKRSLTPYLLLAPGLIWLITFFVIPI
                     FSLASTSTMVRPQGAQIGTYVQELRFANYVDAFLANQEQYVRSFLYAGIATILALAIS
                     YPLAYMIAFKAGKYRNILLVLVVAPFFTSFLLRTVAWSQILGDEGPVVTTLKLLPFFD
                     DSIRLTATPFAVVSGLTYNFLPFMTLPLYASLERIDPRTLEASGDLYASGFTTFRKVT
                     VPLSMPGVVAGTLLTFIPAAGDYVNAALLGNPSTKMIGNVIESRFFAVVDYPTAAALS
                     FTLMATILILVTLYIRKSGTDELV"
     gene            9215..>10008
                     /locus_tag="PLW53_05440"
     CDS             9215..>10008
                     /locus_tag="PLW53_05440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD36898.1"
                     /translation="MSTQVIPRPSFSHRSSRWFSRNLIRIYAALAFVYLFIPVAYTFA
                     FSFNDSGKSNLVWKGFTLDNWKNPCGAPQVCKSVANSLQIGVISTVVATILGTMIAFA
                     ISRHSFRGRKTTNLLIFLPMATPEVVLGASLLAMFLNLRINPGFWTIVIAHVMFCISF
                     VVVTVKARVASLDPRLEQAAMDLYANERETFRYVTLPLVMPGIAGAALLAFSLSFDDF
                     IITNFNSGIESTFPKFVYVSAARGIPAQANVIGSAMFFIALIIVIVG"
BASE COUNT         2821 a         2046 c         2398 g         2743 t
ORIGIN      
        1 gcaatccttg atacaaactg cgatccagat gaagttgatt tcaagattcc tggaaatgat
       61 gatgcaattc gctcagtagc gcttctaact cgcgttattg cagatgctgt tgcagcagga
      121 cttcaagcgc gctcggcaaa tgttgctaaa gaagcaatgg atgctgcaaa gagcgctgaa
      181 ggaagcgttg ctgccggtga gcctcttgca gattgggaga aggagctact tgcaggaact
      241 cctgaagcac caactgagaa ccaaggagca taagtgtcat attcagcagc tgatgtaaag
      301 agacttcgcg aagcaactgc agccggcatg gtcgattgca aaaaagcttt agatgaagca
      361 gagggtgact ttgataaagc cgtagaaatt atccgagtta aaggccttaa aggtgttacc
      421 aagcgtgaag gtagagccac ttcaaatgga cttatcgctg ccaaatcaga gggcaacact
      481 tcggtaatgc ttgaacttaa ttgcgaaaca gatttcgtag ctaagggaga gaggtttcaa
      541 gcagttgcca atgaacttct tgagcaccta ttttcttcaa agcagagcga tcttgcagca
      601 tttctaactt caaagcttgc aacaggtaaa gctgtgcaag aggttatcga tgaggctaat
      661 gcaactatgg gtgagaaagt tgagttaaga cgtcttgcag taattcaagg ctctgcaaat
      721 gctcaatatc tacatcgcac tagcccagat ttgccaccac aagtcggagt attggtagca
      781 cttgctaaag atgatgcagt agttggcaaa gatattgcgc agcacattgc agcatttgcc
      841 ccgcagtact tagatcgagc ttcagtgcct gctgatgtct tagagcatga gcgcaaagtt
      901 gctgaagaga ccgctaagaa tgagggtaag cctgaggcag ctatcgccaa gattaccgag
      961 gggcgcgtga ctggctttat caaagaggtt gctctccttg agcaggcctt tgctaaagat
     1021 cagaagaaga gcgttgcagc gatccttact gaggcgcaaa attccgtgtc agccttctat
     1081 cgattccgag taggacagta aagttatttc cgtagatctc tagatatttt aaggagtggc
     1141 gatatggcag ataagcgaaa ggcctataag cgagtactcc ttaaattatc tggtgaagtc
     1201 tttggcggaa gcaaaggcgt tggagtagat ccagatgtcg tccacggcgt tgccgagcaa
     1261 attgcagagg tagttcgctc cggaactcaa gtagcaattg ttgttggcgg cggaaattac
     1321 tttagaggcg cagaacttca agagcgcggt atggatcgag ctagagctga ctatatggga
     1381 atgctaggaa ctgttatgaa ctgcctagcg ctacaagatt ttctagaaaa agaaggaatt
     1441 gaaacccgag ttcaaaccgc tattgctatg ggtcaagttg ctgagccata tattccacgt
     1501 agatcaattc gccatctaga aaaagggcga gtagttattt ttggcgcagg tgcggggatg
     1561 ccatttttca caaccgatac cgttgcagct cagagagcac ttgaaatcgg agtagatgct
     1621 cttcttcttg caaagagcgg agttgatggg gtctattcag cagatcctag aaaagataaa
     1681 gatgcaaaac gctatgacgt aattacctat gatgaagttt tatcgaaatc acttgctgta
     1741 gccgatgctg cagcatttag cctctgccgc gagaataaat tgcctatcgt ggtatttaac
     1801 cttcttgaaa atggcaatat tgctcgcgct gttcgtggcg aggcaatcgg aacgttagtt
     1861 aattaagggg aatagatgag tattcaaaca gtaattgccg ataccaatac caagatgaat
     1921 aaggctgttg acgttgctaa ggaagatatg gcagcgatta gaactggaag agctcatcca
     1981 tcaatgtttg ccaagattat ggttgagtat tacggaactc tcaccccgct ttcgcaactc
     2041 gcaacaattc aagttccaga ggcaagaaca gcgatgatta ctccatttga taagggagca
     2101 atcacctcaa tagagaagtc aattagagaa tcagatcttg gagttaatcc tgggggagat
     2161 ggcacagtaa ttcgtgttaa tttcccacag ctaactgagg agagacgtaa agagtttatt
     2221 aaggtggcaa aaaccaaggc tgaggattct aagatctcga ttagaagcat tagaagagct
     2281 gctaaagagg ccatggagaa gctagagaaa gatggccaga ttggtaaaga tgatctctct
     2341 cgaggcgaga aagagcttga aaaagtcacc tctgatcatg tggcaaaaat cgatgagatg
     2401 ttaaagcata aggaagccga acttctcgaa gtctaacttt gaggtaaaac tatgagcgac
     2461 cttcactcaa ttaatgaggc gataaataag aaggcggggc gaaagctctt cccctccatt
     2521 ttggtaggtc tattacttct ggcaatagtt ttctccacca tgtcatatgc tccaattgct
     2581 tttgcagcat ttgtgactgt tgcggtattg attgctttaa aagagttaac tgcagcattt
     2641 aaagctcggg gcattacttc aaattatcta gggcttgctc tttcaactgc cttaattctt
     2701 ggaagtactt ggattggcgg acttccaggc ttaactgttt cgatggtagt ttctttaatt
     2761 tttaacttac tccttgttct tcgaggcgga gtagagggct ttgttagtag agctaccgca
     2821 tcagcctttg cacttttata tccaggcttt gtggcaggtt ttattcttct gcttgccaga
     2881 tctggtgagg gattttccta tatcgcaacg ctagtaataa tggttggttg taacgacaca
     2941 tttgcttggg cctttggagt cttgcttgga aaacacccgc ttgctccaaa gatttcacca
     3001 aagaagacct gggaaggttt ctttggtggg ttgatattta gcgcaattgg aagctcactt
     3061 gctttttact atctccttga ttatcaccca gttattggag ctttggcagg atgcgccgga
     3121 gcacttactg caactgttgg cgacctatta gaaagtggca tcaagcgaga tctatcaatt
     3181 aaagatatgg gaacaattct tccaggacat ggcgggatgc tagatcgcct agatgcggtc
     3241 ttaattacag cgccattgct ttggtgtgtt cttgagattt tgaagcgctt tttatgagcg
     3301 agataaaact tgtatttgat gaggttaagc caaagaagaa ggctcctgaa catctagctg
     3361 atttaactat tgctgagcgg cgcgcaaagg cagagtcttt aggccttcca gcctttaggg
     3421 ctaatcagat agcgacccac tacttcacac attacaacga ggagcctgat tcctggagtg
     3481 atattccagc tgcaatgcgc ccactagttg ctgagcactt tctaccaaaa cttctaactc
     3541 ccattcgcca agttgagtgc gataaaggaa agacaagaaa agatctctgg cgcttacacg
     3601 atggagtctt agttgaaact gttttgatgc gttataaaga tagaactaca gtttgtattt
     3661 catcgcaggc aggctgcgga atgaactgtc ctttctgtgc aactggacaa gcaggcttaa
     3721 ctagaaatct aacggcaggt gagattacat ctcaagttgt tgctgcagcg agagcttgcg
     3781 ctagtggaga gttgccaggt ggtccaaccc gcttatcaaa tgtggtcttt atgggaatgg
     3841 gggagccgct tgctaattac aactcagtga tgaagagcgt tagaaacatt attaccccgc
     3901 agccagatgg tttaggaatt ggagcaagat cagtaactgt ttcaacagta ggtctagttc
     3961 caggaattga gaaattaact agcgaagatc tacagattac cctcgctgtc tcacttcaca
     4021 ctccagatga tgaacttcgt gacactttag ttccagttaa ttccagatgg aaggttaaag
     4081 aggtattaag cgcggcagat aaatatgtag ctatgacggg tcggagatat tcaattgaat
     4141 atgccctaat tagagatatc aatgaccaga agtggcgggc agatcttcta ggcaggctat
     4201 taaaaaatcg ccatgcccac gtcaatctaa ttccactaaa tcccactcca ggctctaagt
     4261 ggacggcatc tagaaaggaa gatgaaatag ccttcgttaa aaccttagag aagtatgggg
     4321 ttccggtaac agttcgtgat actagagggc gcgagattga tggagcctgc ggccaattgg
     4381 cagcagctag taagccttaa aagacgctac ttttctctct cttgatagcg ggggcagtaa
     4441 ttaactaaac tctcacgcct gaccaactat gaaaggtttt ttccttgaag acgctacaaa
     4501 actttatcaa cggtaaatca gttccatctt cctcagataa gactcaagac ttgattaatc
     4561 cctcaaccgg ccaagtcttt gctaaagctc cagtttcaaa cgctgctgat atagataagg
     4621 cgatgaaatc tgccgagagc gcatttgaga tttggaaaga gagcacacca ggtgagcgcc
     4681 agaaagcgat caataagatt gctgatgcga ttgaagctcg ctcagaggag ctaatcggaa
     4741 ttgaaagcga gaacacagga aagccaatag cagttactcg cgctgaagaa attggtccaa
     4801 tgcttgatca gattagattc tttgccggag ctgcccgtca tcttgaggga agatccgctg
     4861 ctgaatattc caagggacac acatctttta ttcgccgtga gccaattggt gtctgcgcgc
     4921 aagtaacgcc atggaattat ccaatgatga tggcggtatg gaagtgggcc ccagcaattg
     4981 ctgctggaaa tactgttgtt ctaaaaccaa gtgataccac cccagtttca acgctatgga
     5041 tggcagagct tatgcaagag tttctacctg agggtgtaat aaatgttgta gttggagatc
     5101 gagagactgg aagacattta gtagaacatg agattccgca atttgtctct atcaccggct
     5161 cggttaaagc aggtatggaa gttgcagcaa gcgcagctaa ggatctaaag cgaatccatc
     5221 tagaacttgg cggcaaggct ccagttgtta tctttgatga tgcaaatatt gaggcagctt
     5281 gtgagtggat tgccgttgct ggatatttca atgctggaca agattgcact gcagcaacga
     5341 gagttctagt tgaggcaagt gcctatgaag atgtagttgc gctactaagt gagcaggcta
     5401 aaaacgtgat taaagtaggt atgccagatg aagatgtctt ggtaggacca gttaataacg
     5461 ctagccagct agcaagagtt aagggcttta tagatcgcgc accagcacat gccagaatca
     5521 cagctggtgg aagcgctatt gatcgcccgg gatatttctg gaacccaact gttgttgctg
     5581 atttaagaca agatgatgaa atgattcaga gtgaaatctt tgcaccagtt attacagtgc
     5641 aaaaattcac tgatgaagca gaggcagtgc gttatgccaa tggcgtgaaa tatggacttg
     5701 cttcaagtgt ttggaccaaa gatcatggcc gtgcgatgag aatggccaag gcctttgact
     5761 ttggttgcgt ctggatcaac acccatatcc caatggttgc tgagatgcct catggcggat
     5821 ttaagcattc aggacatggc aaagacctct caggttatgg atttgaggaa tacacgcgca
     5881 tcaaacacgt gatgacaaat ctcaacgcct gatagatttc ccccgaatta gaagtaaacc
     5941 caactagaga aagtgaagag atgagaaaag atccagaaaa gttatcacca gaggcgcgct
     6001 ctatcatccg cgcccaagtc actcgccgct cactactagt tggcgcagga gctattgcag
     6061 gagccagcac ccttgctgca tgcggcacag gatcaactga tagcggagca gagggcgttg
     6121 ttgatgttag tgatagcgaa aagattgttc gttgggctaa ctggccactt tatcttgatt
     6181 acaacgagga tacccaggag tatccaacgc ttaaagcatt tgaagcagct accggaatta
     6241 gcgctaccta caccgaagat attgacgata acaacacctt ctatggaaaa gttcaaggtc
     6301 aattaggaat tggttcagat attggttatg acgttgttac tccaacagat tggatggcag
     6361 ctcgctggat tgaattggga tatgcccagg agctagatgc ggcaaatatt ccaaataaat
     6421 ctaatattcg cgcagttctt gccaatgtgg gctttgatcc aggcagaaat tactcactaa
     6481 cctggcaatc aggttttgct ggatttggct ggaatactca aaagatacct aaaggcgtta
     6541 gaactattga agatctattt gcccctgcca ataagggtcg aattgttgtt ctatcagagc
     6601 tacgcgacac aattggaatt attctgcaat atcaaggcgt agatccttca agtgatttca
     6661 ctgaagatca atatatgaat gcaattgatt tcctaaaggg caaagttgct gacggattta
     6721 ttagaaaagt tcgtggtaat tcatactctg aagatctagt aaatggcgat gcagtagcag
     6781 tcattggttg gtctggagat atgtttatcc tcaagtcaga aaatgatggc aagtttgact
     6841 ttgcaatccc tgaatcaggt ggaactctct ggagcgataa catgctggtt ccttcaactt
     6901 caactcataa gaagaatgct gagcttctaa tgaatcatta ttatgatcca gcaattgctg
     6961 ctgaagttgc tgcatatgta aattacatct gcccagttga agctgctcag agcgaactgg
     7021 aaaaaattga tccagatcta gcagctagcc catttatttt ccctgatgaa gccactttgg
     7081 caaaggttaa ggttttccgt tctctcgatg ctgatgagag cacgaatttc caagcagcct
     7141 ttgacgaagc tgtcggcaac taggggcttg gtagctagcc ggtgatggcg aagaccaaag
     7201 tctcacagga cactggcgac ttagtactaa atcgcatctc caaatattat ggagattttt
     7261 gcgcagtcga tgatctctca ttgaccattc ctgatggttc attctttgca ctccttggac
     7321 catcaggttg tggcaaaacc acaacgcttc gaatgattgc agggcttgaa gagccaactg
     7381 ccggcactat ctctattggt gatactgata tcaccacgca gaagtcatat cgacgcaatg
     7441 taaataccgt ttttcaaaac tatgctctct ttcctcactt aagcatcttt gaaaatatcg
     7501 cctttggtct tagaagacgt ggcattaaag atgttaagga tgaagttgaa aaggcgctca
     7561 atcttgttga gctaggccac ctatctgagc gcaaacctgc tcaactctca ggcggacagc
     7621 aacagagaat cgctgtggca agagcgattg ttaatcgacc agaggtatta ctgcttgatg
     7681 aaccgctagg tgcacttgat ctaaaactga ggcgtcagat gcagattgaa ttgaagcgaa
     7741 ttcaattgga agttggaatt acgtttattc acgtcacaca tgatcaagaa gaagcaatga
     7801 caatggctga cacgattgcg gttatgaatc aaggcaagat tgaacagatg ggctctgcta
     7861 cagaactcta tgaagcacca aagacagcat ttgtggcaaa cttcttaggt cagtgcaatc
     7921 taattaaagt taaagtgcaa tcccgctcag gcggaaaggt ggtagtcagc gctaagggcc
     7981 atacctttga aatcccagag tcaagagttt caagtagcaa tgatgctctc cttcttggag
     8041 tccgtcctga aaaacttcgc cttgatgata aaggtgttaa ctccattaaa gagtgtgttg
     8101 ttaccgaccg ctcctttgta ggaacttcaa cgcagtattt agttagaacc ccttgggatc
     8161 tagagcttgt ggtctttgaa caaaacgatg agggacttca tgatttaagg gttggagata
     8221 aagtctcact tgaatgggat agtgaacaca cctttggttt agatggcgca caagatgtta
     8281 atgctggcgc tgatcttgat gatgacgagt aagtaatggc gtttctgcat ggagcaacca
     8341 agagcaaaag cgggggagga agcgcgctaa agaagcgttc acttacgcca tatctgctgc
     8401 ttgctccagg cttgatctgg ctaattacct tcttcgttat tccaatattt tccctagcta
     8461 gtacttcaac gatggttcgc ccgcaagggg cccaaatcgg cacctatgtg caagagctgc
     8521 gctttgctaa ttacgttgat gcattccttg caaatcagga gcaatatgta aggtcatttc
     8581 tctatgcagg aatcgcaacg attttagctc ttgcaatctc atatcctctt gcatacatga
     8641 ttgcctttaa ggctggtaaa tatcgcaaca tcttgctggt tttagtggtt gctcctttct
     8701 ttacatcatt tctattaaga actgtggcct ggagccagat tctgggagat gaagggccgg
     8761 ttgtaacaac gctaaagctt ctgccatttt ttgatgattc aattcgatta acagcaacgc
     8821 cctttgctgt ggtctcaggg ctaacttata acttcttgcc atttatgact ctgccactat
     8881 atgcaagcct ggagcgaatt gatccaagaa ctttggaagc atcaggtgat ctatatgcaa
     8941 gtggttttac tacctttagg aaggtaacag ttccactttc aatgcctgga gttgtagcag
     9001 gaactcttct tacctttatt ccagcagcag gtgattatgt aaatgctgct cttcttggaa
     9061 atcccagcac caagatgatt ggaaatgtaa ttgaatcaag gttctttgct gtcgttgatt
     9121 atccaactgc ggccgctctc tcatttactt taatggcaac aattttgatt ctagttactc
     9181 tttatatccg taaatctgga acggatgagc tggtatgagt acacaagtga ttccaagacc
     9241 ttctttctcg catcgcagct caaggtggtt ttctcgtaat ctaatcagga tttatgcggc
     9301 tttagccttt gtatatctct tcattccagt ggcctatacc tttgcttttt cctttaatga
     9361 ctcaggcaaa agtaatctag tctggaaagg atttacgcta gataattgga agaatccttg
     9421 cggagctcct caagtatgta aaagcgtggc aaactcactt caaatcggag ttatttcaac
     9481 agtggtggca accatcctag gaacgatgat tgcctttgca attagcagac acagttttag
     9541 agggcgtaaa accactaatc ttcttatctt tcttccaatg gcaactccag aggtagttct
     9601 tggagcctcc cttcttgcca tgtttcttaa tttgagaatt aatcctggat tttggaccat
     9661 agtgattgct cacgtgatgt tctgcatctc ctttgtggtg gtaactgtta aagcaagggt
     9721 tgcctccctt gatccaagac ttgagcaggc tgcgatggat ctctatgcca atgagcgtga
     9781 gacatttaga tatgtcactc ttccgctagt tatgccagga atagcaggag ctgcgcttct
     9841 agcgttttca ctctcatttg atgacttcat catcaccaat ttcaactcag gtatcgagtc
     9901 cacatttcct aagtttgtct atgtctcagc agctagagga atcccagcac aagcaaatgt
     9961 gattggtagc gcgatgttct tcattgccct aatcattgtt attgttgg
//
LOCUS       DAOROD010000029         8365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_533_length_8365_cov_40.131649, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000029 DAOROD010000000
VERSION     DAOROD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_533_length_8365_cov_40.131649"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLW53_05445"
     CDS             <1..635
                     /locus_tag="PLW53_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRD36899.1"
                     /translation="QIVISSDRPPKQLTTLEDRLRSRFEWGLITDIQPPELETRIAIL
                     RKKAAQDKLNAPDDVLEYIASKISTNIRELEGALIRVTAFASLNRQLVDMNLAEIVLK
                     DLIPDDSTPEITASLIMAQTAAYFSLTLDDLCGTSRSRVLVNARQIAMYLCREMTELS
                     LPKIGQTFGGRDHTTVMHADRKVRHLMAERRSIFNQVSELTNRIKLQAKA"
     gene            958..2085
                     /gene="dnaN"
                     /locus_tag="PLW53_05450"
     CDS             958..2085
                     /gene="dnaN"
                     /locus_tag="PLW53_05450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRD36900.1"
                     /translation="MKFTVDSAVLTDSVNWVSRSLSTRPIMTALLGIVIEAGSDITLS
                     ASDLETSAKSSFAAEIKEKGRVLVPGRLLAEISRALPNKPVSFNLDGSRVLVTAGAAK
                     FTLPTLPINEYPNLPVMPDTSGSIASDLFATAVNQVAVAAGRDDSLPTLTGIHVDINK
                     EVITFAATDRYRLAVREINWSPNNPEETTTALLRARTLADAAKTISSSKNISFSVAPN
                     TSNERLIGFTTEQKSMTSRQLDGAFPPYRHLLPSESIATAVIEVATLLDSVRRVALVT
                     DKTVPLRLKFSNNSLHLEAGAGDEATATEELQISYTGEAIDIAFNPTFLADGLNALGT
                     TYVNIAFTGSNKPAVLSGKNKIDGEIDSSYRYLLMPMRYAS"
     gene            2102..3190
                     /gene="recF"
                     /locus_tag="PLW53_05455"
     CDS             2102..3190
                     /gene="recF"
                     /locus_tag="PLW53_05455"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRD36901.1"
                     /translation="MFVSHLSLKDFRSYENLELSLKTGQNIFIGENGEGKTNIIEALM
                     YLALLSSHRISSDQPLIKLGSERAYLRAKIESEERTTLVELEINNGKANRAKVNQNPV
                     RSQKELLGLVKSVCFSPEDLDLVRGDPSERRSFLDQLLIQKSPRLAGVISDYERALKQ
                     RNSLLKSRAPESSLRSWSEHLATFGGELIAGRIKLINEIEPLFQLAYKNLSSKKLAQL
                     GYKSSIPNPTTDKEVNRDSILKVVDEVRTQELERGISLVGPHRDDLLLNLGEHPVKGY
                     ASHGESWSIALALRLASYQLFINEGAKPILILDDVFSELDEGRREKLLEIADSAEQTF
                     ITVAVENDLPKAIKGARFKVELGKVVAL"
     gene            3187..3636
                     /locus_tag="PLW53_05460"
     CDS             3187..3636
                     /locus_tag="PLW53_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRD36902.1"
                     /translation="MKRDIARELYRSYRSGYKKKKEIVEQERSATGEPEKISNLINEL
                     VQNREWRQGIAEGNLFSDWAQIVGSEVAEHATPITLFEGKLTIQTSSTAWSTQLRLIQ
                     SELLKTIRKSSPGALVDELVIMGPHAPSWKRGLRTIRGAKGARDTFG"
     gene            3804..5771
                     /gene="gyrB"
                     /locus_tag="PLW53_05465"
     CDS             3804..5771
                     /gene="gyrB"
                     /locus_tag="PLW53_05465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRD36903.1"
                     /translation="MADKKVGRAYDASAITVLEGLEAVRKRPGMYIGSTGERGLHHLV
                     YEVVDNSVDEALAGYCTHISVLLQKDGGVRVNDNGRGIPVDIHPIEKKPALEVVMTVL
                     HAGGKFGDGGYSVSGGLHGVGISVVNALSRDLSVVVQRDGFIWEQSYKLGVPNSPVEK
                     GIATKETGTEVTFWPSEEIFETTDFSFEILSTRFREMAFLNKGLTISLRDERAGHVDE
                     KGEPLEVRYCYAGGIVDFVKHLNTTKGVAHKSIIAFETEDLKQKMSLEVAMQWNTSFT
                     ESVYTFANTINTQEGGTHEEGFRTSLTSLVNKFAEEWGYIKRKEDRLTGDDIREGLTA
                     IISIKISEPQFEGQTKTKLGNTEAKSFTQKSLNDAVGAWFEKNPSEGKEIVRKAIDAA
                     TARIAARKARDLSRSRKGLLEGRGMPGKLADCQWTEPEKCELYIVEGDSAGGSTKGGR
                     DSRYQAVLPIRGKILNVEKARIDRVLQNNEVQALITALGTGIHDDFDIAKLRYHKVIL
                     MADADVDGQHIRTLLLTLLFRFMRPLIEKGFVYLAQPPLYKIKWGGKEPVQYAFSDKE
                     RDGLIQLGLEAAKRLPKEDGIQRFKGLGEMPAKELWDTTMDPAHRVLMQVTLEDAAAA
                     DDLFSVLMGEDVEQRRQFIQRNAKDVRFLDI"
     gene            5771..8239
                     /gene="gyrA"
                     /locus_tag="PLW53_05470"
     CDS             5771..8239
                     /gene="gyrA"
                     /locus_tag="PLW53_05470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRD36904.1"
                     /translation="MENLDGNKVDRIEAVDLQVEMARSYLDYAMSVIVGRALPDIRDG
                     LKPVHRRVLYAMYDAGYRPDKGYYKSSRIVGDVMGNYHPHGDGAIYDTLVRLAQHWSL
                     RYPLVDGNGNFGSPGNDPAAAMRYTEARMAPLAMEIMRDIDEETVDFIANYDGRSQEP
                     LVLPSRFPNLLVNGSAGIAVGMATNIPPHNLREIAAAVQWALDNPEASNEETLSALLG
                     IVKGPDFPTRGIIVGRQGIEDAYRTGRGSIQMRAVVEVEEINKRTCLVVTELPYQVNP
                     DNLALKIAELVKDGKIKGIADVRDEGNERLGQRLVIVLRNDATPKIVLNNLYKQTQLQ
                     DSFGANMLALVDNVPRTLRLDEFVRHYITHQVEVIIRRTKFRLNEKEKRAHILSGYLK
                     ALDALDAVIALIRASATSEQAREGLMKLLEVDELQANAILDMQLRRLAALERQKIIDE
                     FETLMAEIKELKIILNDPAAQRLIIKEELKEIADKYGDDRRTQIIAGESDLSVEDLIP
                     NQDVVVTITRGGYAKRTKADLYRSQRRGGKGVKGAALKQDDIVEHFFTASTHDWLLFL
                     TNQGRVYRAKVHELPDAGRDARGQHVANLLAFKPNETIASVIGINDYASLPYLVIATK
                     GGIVKKSPLSEYDSPRTGGLIAISLKDGDEVVAASAITAKDELLLISKKGMSLRFTAD
                     DESLRSMGRSATGVIGMKFRSGDELLAMATIRSGDEKSFVLTATDGGFGKRTDISEYR
                     LQSRGGIGVKAAKVDEGARGGLVGALIVNESNEILVITSGGVVMRTPVKEVRQTGRDT
                     LGVRLVNLDQGTFVVSLAKVNDED"
BASE COUNT         2646 a         1641 c         1881 g         2197 t
ORIGIN      
        1 aacagattgt tatctcaagt gatcgcccgc caaaacaatt aaccactttg gaagaccgtc
       61 tacgctctcg attcgagtgg ggtttaataa ccgatattca accgccagag cttgagactc
      121 gtatcgctat tttaagaaag aaagcggcgc aagataaatt aaatgcccca gatgatgttt
      181 tggaatacat cgcttcgaaa atttccacaa atattcgtga gttagaggga gcgttaattc
      241 gtgttactgc ttttgcttct ttaaatcgtc aattagttga tatgaattta gctgagatag
      301 ttttaaaaga tttaattcca gatgattcaa cgcctgaaat aactgctagt ttgattatgg
      361 ctcagaccgc tgcttatttc agtctgactt tagatgatct ttgtggaact tctcgctcta
      421 gggttttagt aaacgcccgc caaattgcta tgtatctctg ccgagagatg actgagttat
      481 cacttcctaa aatcggtcag acttttggcg gccgcgacca cacaactgtt atgcacgcag
      541 atcgaaaagt ccgccacttg atggctgagc gtaggtcgat ttttaaccag gtttccgaac
      601 taacaaaccg gataaaactg caggccaagg cctaatctct gaaatatcag aggttgggga
      661 taacctgtgg atatgtgtgg ataacctgtt ggtaaaggtg gggcttgaaa aggcctgatt
      721 tttccaaatg gtttgtttta agagttacgc acagttttta cacagagaag aggtggttta
      781 aatgaagggt aaagcggcct tgaacagggc ttttgtttct tttccacagt taaaccgttt
      841 ggttactact actaacttta tagataagaa aactcatatg agagaactgc tctttgcaac
      901 aagctttcaa tcaaaccaac tatctaaatt aaagacgaaa gagttaggct gaagattatg
      961 aaattcacag tagatagcgc agttttaacc gatagcgtta attgggtttc aagaagcctt
     1021 tcaacccggc cgataatgac cgcactactt ggaattgtga tcgaagcagg aagcgatatc
     1081 acgctaagtg catcagattt agaaacctca gccaaatctt ctttcgctgc agaaataaaa
     1141 gaaaaaggaa gagtgttggt tccaggaagg ttattagcag aaatctccag agcgctacca
     1201 aacaaaccgg tttcatttaa tctagacgga agcagagttt tagtaacagc aggcgctgcc
     1261 aaattcaccc ttccaaccct tccaataaat gaatatccaa accttccagt aatgcccgac
     1321 acttcaggaa gtattgctag cgatttattt gcaactgctg ttaatcaagt agcagtagca
     1381 gctgggcgag atgactcact accgacctta accggaattc atgttgatat aaacaaagaa
     1441 gttattacct ttgctgcaac cgatcggtat cgcttggcgg ttagagaaat taattggagc
     1501 ccgaacaatc cagaagagac aacaacagcc ctacttaggg cgagaacgct ggcagatgca
     1561 gctaaaacaa tttcatctag taaaaacatt tcattctcag tagcccccaa cacctctaat
     1621 gagcgcttaa ttggcttcac aactgagcaa aaatcaatga cttcacggca gttagatggc
     1681 gccttccctc cctaccgcca cctacttcct agtgaaagta ttgcaactgc agtaatagaa
     1741 gtagcgacac ttttagactc tgtacgccga gtggctttag taaccgacaa aaccgtacct
     1801 ctacgtttaa agttttcaaa taactcactt cacctcgaag caggtgcagg ggatgaagca
     1861 acagcaacag aagaacttca gatctcctac acaggagagg cgatagacat tgcttttaac
     1921 ccaacatttc tagctgatgg tttaaatgcc cttggaacaa cctatgtaaa tattgcattt
     1981 actggctcca ataaaccagc ggttctatca ggcaaaaata aaatagatgg cgagatagat
     2041 agctcttacc gctatctact catgcctatg cgctacgcct cttaattaac aagcgcaaaa
     2101 aatgtttgtt tcccatttat ctctaaaaga ttttcgctca tatgaaaact tagagctttc
     2161 tttaaaaacg gggcaaaaca tctttatagg tgaaaatggc gagggcaaaa ccaacattat
     2221 tgaagcttta atgtatttag ccctactttc atcacatcga atatctagcg atcaacctct
     2281 tattaaatta ggtagcgagc gcgcctacct ccgagcaaaa attgaatcag aagaaagaac
     2341 cacccttgtt gagttagaga taaacaacgg aaaagcaaat cgcgccaaag taaatcaaaa
     2401 tccagtaaga agtcagaaag agttattagg tttagtaaaa agcgtttgtt tctcaccaga
     2461 ggatctagat ttagttcgcg gcgatccgag tgagcgaagg tcttttctag atcaactact
     2521 aattcaaaaa agccctcgtc ttgcgggtgt aataagtgat tatgaaagag ctttaaaaca
     2581 acgcaattca ctactaaaaa gtcgagcccc cgagtcatca cttcgctcct ggagcgagca
     2641 tctagcaacc tttggaggag agttaattgc tggtcgaatt aaattaatta atgaaataga
     2701 accactattt caactcgcat acaaaaatct atcaagtaag aaactggccc aattaggcta
     2761 taaatcaagt attcctaatc ccaccacaga taaagaagta aatagagatt caatattaaa
     2821 agtagtagac gaggtcagga cacaagagct agagcgcgga atttcattag ttgggccaca
     2881 ccgcgacgat ctcttattaa acctaggtga gcacccagtt aaaggttatg ccagccatgg
     2941 cgagtcttgg tcaattgctt tagctcttcg tttagcaagc tatcaacttt ttattaatga
     3001 gggagcaaaa ccaatactta tattagatga tgtcttttct gaacttgatg aaggtcgacg
     3061 agagaaacta ttagagattg cagatagcgc agaacagaca tttattacag ttgctgttga
     3121 aaatgatcta cctaaagcaa tcaaaggcgc taggtttaaa gtagagctag gtaaggtagt
     3181 agctttatga aacgtgatat tgcaagggag ctttaccgtt cttatagaag tggctataag
     3241 aagaaaaaag aaatcgttga acaagaacgc agcgctactg gcgaaccaga aaagatttca
     3301 aaccttataa acgaattagt acagaacaga gaatggcgac aaggaattgc agaaggaaat
     3361 ctatttagtg attgggcgca aattgttggt agtgaggtgg cagaacatgc cacaccaatt
     3421 actttatttg aaggaaaatt aacgattcaa acttcatcaa ctgcttggag cactcaactt
     3481 cgtttgatac agagcgagct tttgaaaaca atccgcaaaa gctctcctgg ggctttagtt
     3541 gatgaactag tgatcatggg tccacacgcc ccttcatgga agcgcggctt aagaacgatc
     3601 cgaggggcaa aaggggctag ggataccttt gggtgatatt ggaggctcaa ctgccttcta
     3661 aaaacgcgcc taatccacca ataacgccct tttttggcta tctctaattt gatttaccac
     3721 taggatttac caatacctag gccaataaag aggcgagaat tgtctctggc ggggtcaaac
     3781 tattgataga acaggggctt agtgtggctg ataagaaggt ggggcgggcc tatgacgcca
     3841 gcgcaatcac cgttctagaa ggcctagaag ctgttcgtaa gcgccctgga atgtatatcg
     3901 gctcaacagg tgagcgaggc ctacaccact tagtttatga agtcgttgat aactcagtag
     3961 atgaagctct agccggctac tgcacccaca tcagcgttct attgcaaaaa gatggcgggg
     4021 ttcgagtaaa cgataacgga cgtggaattc cagtagatat tcatccgatt gaaaagaaac
     4081 cagcgctaga agttgttatg acagtacttc acgccggagg aaagtttggc gatggcggct
     4141 actcagtatc tggtggctta cacggcgttg gaatatctgt tgttaatgct ttaagcagag
     4201 atctaagtgt tgtagttcaa agagatggtt ttatctggga acagagttat aaattaggag
     4261 ttcctaactc tccagttgaa aaaggtattg ctacaaaaga aactggaact gaagttactt
     4321 tctggccctc tgaggaaata tttgaaacta cagatttttc atttgagatt ttgtcaacca
     4381 gatttagaga gatggccttt ttaaataaag gtttaactat ctcgctgcgc gatgaacgag
     4441 caggacacgt tgatgagaaa ggtgagccac tagaagttag gtattgctac gcaggcggaa
     4501 ttgttgattt cgttaaacat ctaaacacca caaagggtgt tgcccataaa tcaattattg
     4561 cctttgagac agaagacctt aaacaaaaaa tgtcgcttga ggtagcaatg cagtggaata
     4621 ccagttttac cgagtcggta tatacctttg ctaacaccat caatactcaa gaaggcggaa
     4681 cacatgaaga aggttttcga acttcgctta cctcactagt aaataagttt gccgaggagt
     4741 ggggttatat aaagagaaaa gaagatcgcc taactggaga tgatatccgc gaagggctaa
     4801 ctgcaatcat ctcaattaaa atcagtgagc cacaatttga aggacaaaca aagacaaaac
     4861 taggaaacac cgaggctaag tcattcaccc aaaaatcttt aaatgatgca gtaggtgcat
     4921 ggtttgaaaa gaatccaagt gaaggtaaag agattgtaag aaaagctata gatgcagcaa
     4981 ctgctcgaat tgcagcaaga aaagccagag atctttctcg gagcagaaaa ggcctacttg
     5041 aaggtcgcgg aatgcctgga aaattagcgg attgccaatg gactgagccg gaaaagtgcg
     5101 agctctatat tgttgaggga gattcagctg gtggttcaac aaagggcggg agagattctc
     5161 ggtatcaagc agttcttcca attagaggca aaatattaaa tgttgagaaa gcaagaattg
     5221 atcgggtgct acaaaacaat gaagttcaag ctttaatcac cgctcttggc acaggaattc
     5281 atgatgattt tgatatcgcc aaacttagat atcacaaagt tattctcatg gcagatgccg
     5341 atgtcgatgg acaacatatt agaacgctac tgctaactct cctatttaga tttatgcgac
     5401 ctttgattga gaaaggcttt gtatatcttg cacaacctcc gttatataaa atcaaatggg
     5461 gcggaaaaga acctgtgcag tatgcattct ctgacaaaga gcgagatggc ttaattcaat
     5521 tagggctaga ggcagcaaag cgtttaccaa aagaggatgg aatccagcgc tttaaaggcc
     5581 ttggtgagat gcctgcaaaa gaactttggg acaccacaat ggatccagcc caccgcgtgc
     5641 tgatgcaagt gactttagaa gatgcagcag cagctgacga tctcttctca gttctaatgg
     5701 gtgaagatgt tgaacagaga agacaattta ttcaaagaaa tgctaaagat gttaggttcc
     5761 ttgatatcta atggaaaatc ttgacggaaa taaagtagat cgcattgaag ctgttgacct
     5821 tcaagtggaa atggctcgtt cttatcttga ttatgcgatg tcagtcatcg ttggaagagc
     5881 acttcctgat atcagggatg ggttaaaacc agtacatcgc agagttttat atgcgatgta
     5941 tgacgcggga tatcgcccag ataagggtta ttacaaatca tcacgcatcg tcggtgatgt
     6001 tatgggtaat taccatccac atggcgacgg cgctatctat gacaccttag ttcgtttagc
     6061 tcaacattgg tcacttagat atccattagt tgatggcaat ggaaactttg gatctcctgg
     6121 taatgatcca gcagccgcaa tgcgttacac agaagctcgt atggcccctc ttgcaatgga
     6181 gatcatgcga gatattgatg aagaaactgt tgatttcatc gccaattacg atggacgaag
     6241 ccaggagcca ttagtacttc catcccgctt tccaaattta ttagttaatg gctcagctgg
     6301 tatcgctgtt ggaatggcaa ccaatatccc gccacataat ctgcgcgaaa tcgcagcagc
     6361 agtgcaatgg gctcttgata acccagaagc atctaatgag gagacactaa gcgctcttct
     6421 tggaattgtt aaaggacctg actttccaac tagaggaatc attgttgggc gccaaggaat
     6481 tgaagatgct tatcgcacgg gccgcggctc tatacagatg cgtgcggtcg tggaagttga
     6541 agagattaac aaacgaacct gtctagtagt aacagagctg ccatatcaag taaatcctga
     6601 caaccttgct ctcaaaatcg ctgagctagt aaaagatgga aaaatcaaag ggattgctga
     6661 tgtaagagat gaaggaaatg agcgccttgg tcaacgttta gtaattgttt tgagaaatga
     6721 tgctactcct aaaattgtct taaataatct ttataagcaa acccaactcc aagattcttt
     6781 cggagccaac atgttggcct tggttgataa cgtcccaaga actcttcgcc ttgatgaatt
     6841 tgtccgccat tacataactc atcaagttga agtaattatc agacggacga aatttagatt
     6901 aaatgaaaaa gagaagcgcg ctcatatttt aagtggttac ttaaaagctt tagatgccct
     6961 agatgcagta attgccttaa ttagagctag cgctacatca gagcaagcgc gtgaaggttt
     7021 aatgaagctt cttgaagtag atgaactcca agctaatgca attctagata tgcagctgcg
     7081 tcgccttgcg gcccttgaac gccagaaaat tattgatgaa tttgaaactt taatggctga
     7141 aattaaagag ttgaagatca ttcttaacga tcctgccgct caaagattga taatcaaaga
     7201 agaactaaaa gaaattgcgg ataaatatgg cgatgatagg cgcacccaaa tcattgcggg
     7261 cgagagtgat ttatcagttg aagatttaat tcctaaccaa gatgtagttg tcacaatcac
     7321 tagaggcgga tatgccaagc gaactaaggc agatctatat aggtcgcaaa gacgcggtgg
     7381 aaaaggcgta aaaggtgcag ctttaaaaca agatgacata gttgaacatt tctttactgc
     7441 atcaactcat gactggctcc tctttttaac aaatcaaggc cgggtctata gagccaaagt
     7501 ccatgagctt ccagatgctg gtcgtgatgc aagaggccaa catgttgcaa acctattagc
     7561 ttttaagccc aatgaaacca tcgcctctgt tattggaatc aacgattacg catcacttcc
     7621 ctatttagtg attgctacaa aaggcggaat agtaaaaaag agcccactta gtgaatacga
     7681 ttcaccaaga actggcggtt taatagcaat ttcattaaaa gatggcgatg aagttgtggc
     7741 tgcctctgct attactgcca aagatgagct acttttaatt tcaaagaaag gaatgtcact
     7801 tcgattcaca gctgatgatg aatctctacg ctctatgggt agatctgcaa ctggcgtaat
     7861 tggaatgaag tttagaagcg gtgatgaatt gcttgctatg gcaacaatta gaagtggcga
     7921 tgaaaagagt tttgtattaa cagcaacaga tggtggtttt ggaaagcgaa ctgatatcag
     7981 tgaatatcgt ttgcaatccc gaggcggcat tggtgtaaaa gctgccaagg ttgatgaagg
     8041 ggcgcgaggc ggtctagtcg gcgcactgat tgttaatgaa agtaatgaga ttttagttat
     8101 tacctcagga ggcgtagtta tgcgcacccc agttaaagaa gttcgccaga ctgggcgcga
     8161 tactctcgga gttcgcctgg taaatcttga tcaaggcacc tttgtagtct cattagctaa
     8221 ggtcaatgac gaggattaga aagtgaaaaa ataagcggaa aaaaagtgct atttttgcgc
     8281 acgcccgcag gtcaggtagc cttgcgcttc ccgttttgcg ggcctatagc tcagcctggt
     8341 tagagcgctt ccctgataag gaaga
//
LOCUS       DAOROD010000030         8125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_566_length_8125_cov_31.790706, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000030 DAOROD010000000
VERSION     DAOROD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_8125_cov_31.790706"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLW53_05475"
     CDS             <1..368
                     /locus_tag="PLW53_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD36905.1"
                     /translation="DKFAQRTGQPRELLVEITAKAHKVWANEPRMTKRELKKIKCPTL
                     ILAGDDEPFSTEHTASLYEAIPGAQLAILPGTSHAVLKEKPKLVKQILKDFYKNPGMP
                     ITLSPNRRQKQSLGATTNS"
     gene            complement(345..2039)
                     /locus_tag="PLW53_05480"
     CDS             complement(345..2039)
                     /locus_tag="PLW53_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRD36906.1"
                     /translation="MASKVQEFDVVVVGSGFGGSVAALRLTEKGYRVAVIEAGRRFED
                     KDFPKTSWRLSRFLYAPRLGLRGIQRIHALPDVLILAGAGVGGGSLVYANTLYTPPDS
                     YFEDKQWAGITDWKSELAPWYDQASRVLGVTKNPYFSASDQAMKDVAEEMGVGDSFKM
                     APLGVHFGSGPKVLEKDPYFGGVGPDRNGCQQCGGCMTGCRFNAKNTLPKNYLGLAEK
                     AGATVFPELTVESFEQIENGDWKITARGSSSWFGAKKVFIAKDLVLAAGTYNTQKLLH
                     RMRDKGSLPKLSPTLGSLSRTNSEALTGAIMPRNSAIDFSKGAAITSSFFPDENTHVE
                     PVRYGKGSNLMGLLQTIMTDGSAAKQRRRNWIGNAIKNPLLFFKIFDVRSWSQRTVIA
                     LVMQNVDSSIKVFSKKGLLGWRLTSKNDSDRPNATYIPAANEVARRIADKYGGIAGGH
                     VGDLISAPFTAHFVGGCVIGSDESKGVIDPYHRVWNYPSLHVVDGSALTANLGVNPSL
                     TITAQAERAFSLWPNKAELDPRPAQKSPYKRIQAVLPNKPFVPEHAYGALRVSSSS"
     gene            complement(2067..2924)
                     /locus_tag="PLW53_05485"
     CDS             complement(2067..2924)
                     /locus_tag="PLW53_05485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRD36907.1"
                     /translation="MSSPLSFVVLGDSAAYGTGDITKADRPMGWTYRIASAITGPMTY
                     LNLARPGAKSSELVEHQLPIATLLRPDLAIVVVGGNDMLRNNFCPIKLKENLTEIFTR
                     FSALGTHVITLELHDPSKVLKLPKALERALLKRVDAVNAVYQELAESFPIIPIRARDI
                     AGVHDRKNWHVDLLHPGPRGHYLLAEAAVRELARRGLPIKALDVPEFPTISTREKVSW
                     MLLKGTPWFFKRSFDLLPVALFLIAKELILSGVSRLRAKEPTKKIEKAQEIRHKSSNE
                     GKELISSAA"
     gene            complement(2921..4060)
                     /locus_tag="PLW53_05490"
     CDS             complement(2921..4060)
                     /locus_tag="PLW53_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD36908.1"
                     /translation="MKILHIANFYGPKSGGIKTTIHDLGKRYEKAGHEFTFVVPGINL
                     SKTKTPYGTALYLPSVEIPFSGGYRIFRRRREIKQAIVVMKPDRIECSDRFTLLFIGP
                     FARRMGIQTLVFSHETLTGLFSKFLPAQKTFQRIADWHNRRLAKAFDYVIATTRFASK
                     EFERLSTANLRLIPLGVDSSTFNPKRYKSELRKKLLNGKEHLIVHCGRLSPEKDPHLS
                     IEALEILTRQMGVSAQLVVIGGGPLSEKLKSHSAHLPVSFTGYIANVELIADIFSVAD
                     VSLAPGPLETFCLSALESLSSGTPVVANSRSAVGEFLGEKSSNPCGATASDSYEFALK
                     IKAILEGKSLRKAARAKALEFTWEKTMNNLLTLHNTPKVEVAQAA"
     gene            complement(4057..5169)
                     /locus_tag="PLW53_05495"
     CDS             complement(4057..5169)
                     /locus_tag="PLW53_05495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRD36909.1"
                     /translation="MRVAIVTESFLPTINGVTNSVIQVNRTLVSQGHQVVILAPDVNS
                     RPKFFEGSEVIGLPAINLQKVLPVAVPNFQIQRALLDFRPDVIHLASPAIMGAHAQRV
                     ASKLAIPTVAIYQTDYVGFAQHYKFSFAQRAIEKIVRVIHQSATINLAPSTFAIAQLD
                     KAGVNNVARWGRGVDLEQFNPKHRRDDLFPRDKFVLGFVGRLAPEKGIERLAALDSRD
                     DIQLVIIGDGPMRTELERKLPSAIFTGKKSGLELGQHFASLDAFIHPGEHETFCQAAQ
                     EALASAVPVIAPTRGGIRDFILEGLNGILVDFQDKSALANIPSSKWQFLSDPQTKDAA
                     RESVKDRSWESVNQELIGHYQSVVAQSRAKRVGLAS"
     gene            5328..6791
                     /locus_tag="PLW53_05500"
     CDS             5328..6791
                     /locus_tag="PLW53_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD36910.1"
                     /translation="MGKPAVIMITSNGVGAGHLIRASAIARALQSDARPIIFSMAYSV
                     VEVAKALDLDCEYVPGRDKGLMPRKKWDSYLRDRLIALIDETGASVITFDGVVPYPGI
                     IAAKIARPSVNLIWIRRGMWQRKPQGMVLGLQSRLMDYVIEPGDVARDADSGPTKSRL
                     ESVLTKPVTLYLPERALSRQDARRFLGLDNDKPAVLVQMGVGNTDLDARASAVFKGLS
                     GWKDLQIVMPRKPVDQEGNSLVPEGTDVKVIRHFPLADLLHAFDAAICAAGYNSVHEV
                     IPAGIPTLFIANNRGTDDQKSRAKWCAEQELAIYADNESLEDIESQSARLQSQALREQ
                     LTAKCSSVEDFSGAAQIAELINLLSNQAYSSLINKRLTYQRFIYFAAFARSPKFYARR
                     FINLLLRAAAIAFRTLFPHDGPTETNGEVVFSDSRNFKILDKYIRTQERFEHLVKDFS
                     PSYLAKRKAIAKAAFGEVDMNPILDRSEIEAHLRFAS"
     gene            complement(6775..7671)
                     /locus_tag="PLW53_05505"
     CDS             complement(6775..7671)
                     /locus_tag="PLW53_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD36911.1"
                     /translation="MSQFSDALFIGGSGRSGTTIAGKLLSRHQKVILAKPIEIKFLTS
                     GNGLLDLYLNPRISRSGKVRVGPNANLKRFIKSADDKWWFRKGKKSGVTGLSTGIERA
                     EWDALIANLQAEIEENRFQACQNFFRGYLDIQKREATSHLWIDTTPPNLMRANEIAHL
                     LPGARFLHMIRDGRDVASSVVKEPWGPSKHFEALDWWKERMLLILKETRDMESSVLHL
                     WMEDLVENDREASLEKLLKFLNLGPDSKMFDYFRETINPSSAHTGRWRQEVKRASEFD
                     SKYQKILEGLIKEGLKLPTKQT"
     gene            complement(7888..7962)
                     /locus_tag="PLW53_05510"
     tRNA            complement(7888..7962)
                     /locus_tag="PLW53_05510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7928..7930),aa:Thr,seq:tgt)
BASE COUNT         2226 a         1883 c         1702 g         2314 t
ORIGIN      
        1 aagataaatt tgcccagaga actggccaac caagagagct tctagttgag ataacggcaa
       61 aagctcataa agtttgggca aatgagccta ggatgactaa gagagaatta aagaaaatta
      121 agtgccccac cttgatttta gctggcgatg atgagccatt ttcaaccgaa catacggcat
      181 cactttatga agcaattcca ggggctcaat tagcgatact gcctggcaca tctcatgctg
      241 ttttaaagga aaaacctaaa ttagttaagc agatattgaa agacttctat aaaaatccag
      301 ggatgccgat aactctctcg ccaaatcgca gacaaaaaca gagcctagga gctactacta
      361 actcttagtg cgccataggc atgctctggc acaaacggct tattgggaag cacagcttga
      421 attcttttat atggactctt ttgtgcaggg cgaggatcta gctctgcttt atttggccag
      481 agcgaaaatg ctcgttctgc ctgggctgta atagttagag atggattaac accaaggttt
      541 gcagtcaggg ctgaaccatc gactacatga aggctcggat aattccagac tcgatgatat
      601 ggatctatta ctcctttact ctcatcactt ccaattacac aacctccaac aaagtgagca
      661 gtaaaggggg cgctaattag atctccaaca tggcccccag caattccgcc atacttgtca
      721 gcaattcttc tagctacttc atttgccgct ggaatgtagg ttgcatttgg tctatcgcta
      781 tcgttttttg aagttaagcg ccaaccaagt aatcccttct ttgaaaagac tttaattgag
      841 gaatccacat tctgcatcac aagtgcaatt acagtccttt ggctccagct tcttacatca
      901 aagatcttaa aaaacagtaa aggattttta attgcatttc ctatccaatt tcttcgcctc
      961 tgcttagctg cgcttccatc agtcataatt gtctgaagca gccccattaa attagagcct
     1021 ttgccatatc taactggctc aacatgggtg ttttcatctg ggaaaaaaga tgaggtgatt
     1081 gcagcgcctt ttgagaaatc aatcgcgcta tttctaggca ttattgcccc agttaacgct
     1141 tcagaattag tcctagataa gctgccaaga gttggcgata gctttggtag cgatccttta
     1201 tctctcatac gatggagcaa tttctgggtg ttataagttc ctgctgctaa gactaaatct
     1261 ttagcgataa ataccttttt agcaccaaac caggatgagc tacctcttgc ggtaattttc
     1321 caatctccat tttcaatctg ctcaaagctc tcaactgtta gctctggaaa aactgtcgct
     1381 cccgcctttt cagcaagtcc taggtaattc tttggcagag tatttttagc attgaagcga
     1441 caacctgtca tacatccacc acattgttgg cagccatttc gatctggccc tactccgcca
     1501 aagtaaggat ctttctctaa aacttttggt cctgatccaa agtgcacgcc aagtggagcc
     1561 attttaaaac tatcgccaac acccatttcc tctgcaacat ctttcatggc ttgatcagag
     1621 gcagagaagt aaggattttt agttactcct aaaactcttg atgcttggtc ataccaggga
     1681 gcaagctctg atttccaatc agttatgccg gcccattgtt tatcttcaaa gtaagaatca
     1741 ggcggggtat ataaagtatt tgcataaact aatgaaccgc cgccaactcc agctccagct
     1801 aaaatcaaaa catcaggcag ggcatgaatc cgctgaattc ctctcaaacc aagtcgagga
     1861 gcatagagaa atcttgataa tctccaagaa gtcttaggaa aatccttatc ttcaaatctt
     1921 cttccagcct caatgacagc gactcgataa cccttttccg ttaaacgaag cgccgcaaca
     1981 gatcccccaa agccagagcc aacaactaca acatcgaact cttgaacttt gctagccata
     2041 gccagaaggc tacaccgagc ccttttttag gcagcgctgc tgatcaattc cttgccctca
     2101 ttgctgcttt tatgccttat ctcctgggct ttttcaattt tctttgttgg ctcttttgcc
     2161 cttaatcgac ttactccgct cagaattaat tcttttgcga ttaaaaatag cgcaacagga
     2221 aggagatcaa aagatctttt gaagaaccaa ggggtgccct taagaagcat ccacgagact
     2281 ttttctcttg ttgagattgt aggaaactct ggaacatcaa gtgctttaat tggtaaacct
     2341 cttcttgcca actctcttac tgcagcctca gctaagagat agtggcccct cggtccgggg
     2401 tgaagtagat ctacatgcca attctttcta tcgtgaactc ctgcaatatc tcttgctctg
     2461 ataggaatga taggaaaact ctctgcaagt tcttgataaa cagcattaac agcatcgacg
     2521 cgtttaagca gggcgcgttc gagtgcttta ggaagtttta aaactttgga aggatcatgt
     2581 agctctagtg ttattacatg agttccaagg gcgctaaatc gagtaaatat ctcagtgaga
     2641 ttctctttta atttaatcgg acaaaagtta tttcttagca tgtcatttcc cccaacaaca
     2701 acaattgcca ggtcaggacg gagcaaggtt gcaatcggga gttgatgctc aacaagttct
     2761 gatgatttag ccccgggcct tgcaagattt agatatgtca tcggcccggt aatggcgctt
     2821 gcaattcgat atgtccatcc catcgggcga tctgcctttg taatatctcc agtgccataa
     2881 gctgcgctat caccaaggac aacaaagctc agtggactac tcatgctgcc tgagctacct
     2941 caactttagg ggtgttatga agagtcaaga gattattcat cgtcttctcc caagtaaatt
     3001 ccagcgcctt agcccttgcc gcctttctca atgattttcc ttccaaaatt gctttgatct
     3061 tcagcgcaaa ctcatagcta tcgctagctg ttgccccgca gggattacta cttttctcac
     3121 ctaaaaactc cccaactgct gagcgtgaat tggcaactac tggagtgcca gagcttaaag
     3181 attcaagagc tgacagacag aaagtctcta ggggacctgg agcaagggag acatcggcta
     3241 cagagaagat atctgcaatt aattcaacgt ttgcaatata accagtaaag ctaactggaa
     3301 gatgagctga atgactcttg agtttttcag ataacggccc accaccaata acaactagtt
     3361 gcgcagaaac ccccatctgc ctggtcaata tctcaagtgc ttcaattgaa aggtgtgggt
     3421 ctttctctgg agagagtcgg ccacaatgaa cgattaaatg ctctttgcca tttaataact
     3481 ttttgcgaag ctctgactta tagcgctttg gattaaaggt gcttgaatca acacctagcg
     3541 gaatcaatct caagtttgcc gtgctcaatc gctcaaactc ttttgaagca aaacgagttg
     3601 tggcaataac atagtcaaag gctttagcca gacgacgatt atgccaatct gctatgcgtt
     3661 gaaaagtttt ttgagcagga aggaacttac tgaaaagtcc tgtgagagtt tcatggctaa
     3721 agaccagagt ctgtattccc atccgtcttg caaacgggcc aataaataag agcgtaaagc
     3781 gatcggagca ttcaattcga tctggcttca tcacaactat tgcctgctta atctctcttc
     3841 ttcttctaaa gattcgatag cccccagaaa atggaatctc aacgcttggc aaatagagcg
     3901 ctgttccata tggcgtcttt gtcttggaga gattaattcc tggaacgaca aaggtaaatt
     3961 catggcctgc cttctcatat ctcttaccca agtcatgaat tgtggtcttg attcccccgg
     4021 atttagggcc atagaaatta gcgatatgta atatcttcat gaggctaggc caactctctt
     4081 cgctctagat tgggcaacaa cgctttgata atggccaatt aactcttgat ttacgctctc
     4141 ccagctgcga tctttaacag attctctagc tgcatccttg gtttgaggat ctgataaaaa
     4201 ctgccacttg gagcttggga tattggccag tgcgctctta tcttggaaat caaccaagat
     4261 tccatttagc ccttcgagga taaagtctct gatcccgccg cgagttggag caattacagg
     4321 aactgctgat gcaagcgcct cttgagcagc ctgacagaaa gtctcatgct cgcctggatg
     4381 aataaatgca tcaaggcttg caaagtgttg acctaactcc aagccacttt tctttccagt
     4441 aaagattgct gaaggtaatt tgcgctctag ctctgtgcgc attggaccat caccaatgat
     4501 tactaattga atatcatctc ttgaatcaag ggctgcaaga cgctcgattc ctttctctgg
     4561 agcaaggcgt cccacaaatc ctaaaacaaa cttatctctt ggaaataggt catctctgcg
     4621 atgcttggga ttaaattgct ctagatccac acctcttccc catcttgcaa cattattaac
     4681 gccggcttta tccaattgcg ctatcgcaaa tgtagatggg gcaagattga tagtggctga
     4741 ttgatgaatt actcgaacaa ttttttctat cgccctttga gcaaatgaga atttatagtg
     4801 ttgagcaaag ccaacataat ctgtctgata gatggcaact gttggaattg ccaatttact
     4861 tgccactctc tgggcgtgag ctcccataat tgccggagag gctaggtgaa ttacatcagg
     4921 tctaaaatca agtagcgctc tctggatttg gaagttaggt acagctaccg gcaatacttt
     4981 ctgcaggtta attgctggaa gaccaataac ctctgagcct tcaaagaatt tagggcgaga
     5041 gttaacatcg ggcgccaaaa tcaccacctg gtggccctgg cttactaagg tgcgattaac
     5101 ttggattaca gagtttgtta cgccatttat cgttggcaga aatgactctg tcactatcgc
     5161 cactctcatg ccttaagggt ctatctttag atttccccaa aattgacggc agagaaagtg
     5221 ttggaaaacg ctgagtgaag aggtcaactg ggttttcccg cgtgaccttc aatccttgtt
     5281 aacctgatag ccacgtctcg ttcaaaaact gctcctacct tgctctagtg ggaaagcctg
     5341 cagtcataat gattaccagc aatggagtgg gcgccggaca tctaattcga gctagcgcaa
     5401 ttgccagagc cttacaaagt gatgcgcgac caattatctt ttcgatggct tactctgttg
     5461 ttgaggtggc aaaggctctc gacctagatt gtgagtatgt tccaggaaga gataaaggat
     5521 taatgccgcg aaagaaatgg gatagctatc ttcgagatcg actgattgcg ctaattgatg
     5581 agaccggcgc ctctgtcatc acttttgatg gagttgttcc atatcctgga attatcgctg
     5641 caaagattgc ccggcctagc gttaatttga tttggattag aagagggatg tggcagagaa
     5701 aacctcaagg aatggtccta ggtctgcaat caaggttgat ggattatgtg attgagcctg
     5761 gagatgttgc tcgcgatgca gattctgggc caaccaagtc aagacttgaa tcagtattaa
     5821 caaagccagt gactttatat cttcccgagc gcgccctttc gcgccaagat gctcgcaggt
     5881 ttcttggcct tgataacgat aagcctgcag tacttgtaca gatgggggtc ggaaatacag
     5941 atctagatgc tcgagcatct gctgtcttta aaggattatc tggctggaaa gaccttcaga
     6001 ttgttatgcc tagaaaacca gttgatcaag agggtaactc ccttgttcct gagggcacgg
     6061 atgtaaaggt aatcagacac tttcccttag ccgacctact tcatgccttc gatgccgcta
     6121 tttgcgcagc tggatacaac agcgtacatg aagttattcc ggcaggtatt cccactctat
     6181 ttatcgcaaa taatcgcgga acagatgatc aaaagtctcg cgctaagtgg tgcgcagaac
     6241 aagagcttgc aatttatgcc gataatgaat ctttagaaga tattgagagc cagagcgcca
     6301 gattgcaatc ccaagccctg cgagagcaac tcacagccaa gtgcagctcg gttgaagact
     6361 ttagcggcgc tgctcagatt gctgagctaa taaatctctt atcgaatcag gcctatagct
     6421 ctcttataaa taaacgtctt acctatcaac gatttatcta tttcgcagct tttgctcgca
     6481 gtcctaagtt ctatgcccgt cgcttcatta acctcctact tcgcgcggca gcaattgctt
     6541 ttagaactct ctttcctcac gatggaccta ctgagacaaa cggggaggtg gtctttagcg
     6601 attcaagaaa tttcaagatt ttggataaat acatcagaac tcaggagcga tttgagcatc
     6661 tagttaaaga cttttctcct tcttatcttg ccaagagaaa ggcgatagca aaagctgctt
     6721 ttggagaggt cgatatgaat ccaattttgg atcgtagtga aattgaggcg cacctcaggt
     6781 ttgcttcgta ggaagtttaa gtccctcttt aattaatccc tccagaattt tctgatactt
     6841 cgaatcaaac tctgaagctc tctttacctc ttgcctccag cgacctgtgt gggctgaaga
     6901 aggattgata gtttcgcgga agtagtcgaa catctttgaa tctggcccta gatttaagaa
     6961 ctttagtaat ttttcaaggc ttgcttctct atcattttca actaaatctt ccatccagag
     7021 atgaagtacc gagctctcca tatctcttgt ttcttttaga attaacaaca ttctctcctt
     7081 ccaccaatct aaagcctcga agtgctttga agggccccat ggctctttga ctactgaact
     7141 tgccacatca cgtccatctc gaatcatgtg aagaaatcgc gcaccaggta atagatgagc
     7201 tatctcatta gcgcgcatca gatttggagg ggtagtgtca atccagaggt gagaggttgc
     7261 ttccctcttt tgaatatcta gatatcctct aaagaaattc tgacaggctt gaaatctatt
     7321 ctcttctatc tctgcctgta gatttgcaat taatgcatcc cactcggctc gctctatccc
     7381 cgttgataac cctgtcactc ctgacttctt gcctttgcga aaccaccact tatcatcagc
     7441 gctctttata aatctcttta gatttgcatt gggcccaacc ctaacttttc cacttctact
     7501 aatccgtggg ttgagataga gatcaaggag accatttcct gaagttaaaa actttatttc
     7561 aataggtttt gccaaaataa ctttttgatg cctagagagc aatttgcccg caatcgttgt
     7621 tccacttcgc ccactgccac caataaagag cgcatctgaa aattgactca tgggagaaaa
     7681 tcctttgcca aagacataga ctgcgattat tcctcaattg agaactgaaa acgggtggaa
     7741 tgaccctcga ttcattacca cccgttatcg cagtatctat acttagcttg cctttaggag
     7801 caagtcgggc gtgagtctgg attggtgagg tgattagcaa agggcttctg ggtaaattca
     7861 aggagaattt tgaaagtagt ttttctctgg agccccccgt caggattgaa ctgacgacct
     7921 tccgcttaca aggcggatgc tctaccactg agctagggag gcgttttcag cgagtaagta
     7981 tggcattgag caagaaaatg gaacaatgcg ccaaccatgc caacgccaat ctggtctagc
     8041 cctgagacta cctcagtaaa ccgcttgcca atgctcaata tcgcgcatct gatgagtatt
     8101 tctcttgatg ggcaatggaa ctttc
//
LOCUS       DAOROD010000031         7008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_753_length_7008_cov_21.155041, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000031 DAOROD010000000
VERSION     DAOROD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_7008_cov_21.155041"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLW53_05515"
     CDS             <1..605
                     /locus_tag="PLW53_05515"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRD36912.1"
                     /translation="GDTALRTGIEGAEDVLEGAIEDLKKLNLSKEDSLVGIAASGRTP
                     YVVGALQYAQKVGAASIALSCNLNSELSKFADVAIEVDTGAEILAGSTRLKAGTAQKL
                     VLNMISTVSMINLGKTYGNLMVDLQVSNIKLRDRAIRIIQSATNCDTRRAQEALEESK
                     DQVKVAIVMILLDTSAEKALELLKASGSRVRAALGKPANT"
     gene            678..1607
                     /locus_tag="PLW53_05520"
     CDS             678..1607
                     /locus_tag="PLW53_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRD36913.1"
                     /translation="MAKQLSLVTPSHHSNLLELIQSQLGSFHASEIAIAEVILKDPQL
                     IAPMNITQLATHSKTSVSSVVRFSKALGFRGFPEFKMALVSDLSWKSAKQFDTEYLDG
                     GITPSDSLASIIEKVTYSDARAIESTAEKLDIQTFKKVVEIFEKAKTIGLFGLVSSGF
                     VAMDFQSKLNRLSKRAISWTDFHSAMTSIAVLDKGDVLVAITHSGTTVDTVDVIREFK
                     ARGVTIVLITNTLRSPAASLADFVLHTAARETTFRSGATASRIAQLTVVDCLCVALAQ
                     RNWSGTKSALDLSRAAVSIRSGKNHTYRPRGGN"
     gene            1608..2882
                     /locus_tag="PLW53_05525"
     CDS             1608..2882
                     /locus_tag="PLW53_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD36914.1"
                     /translation="MKISKIRGLVAALFAAVTVVAPGASIASANTEITFWSWRTEDKA
                     FYESQMRLFEAKNPGITVKFTPYLNTEYNAIVATALTAGKGPDVLQMRPYGGMANLSD
                     AGNFMPLTTAEVPALKKLPAGILAGAQGYKDKKQYGLPYAVSALGVMYNPDILEKAGV
                     RALPQTWEQMLSAFDKVKKAGFLPIGNAGGNGPALEQLHATVGPTFYGGTAFFNDVVA
                     GKKTFTDKGYVASLKAVADLAKWMPANYQGIDYNTARGLFANEKAAFYIGGNYEIGYF
                     RSLNPSITIGWFPAPKANAGAKRYVGTWADGAYAINAKTTQKAAALKLINFLGTKEFG
                     QALVDEIAFIPTAPYVKINDPAVKEINRWRTTLGTPFLNVVGFRFENPTSSSILQPGF
                     QKLIVGGTTPEQLAKDVQTAVASWYAPQKGKS"
     gene            2945..3862
                     /locus_tag="PLW53_05530"
     CDS             2945..3862
                     /locus_tag="PLW53_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD36915.1"
                     /translation="MSSALRREQNRTTGTLLIPALAVIIIFTTVPALTTLSYAFFSWD
                     GFQRSSFAGFENFQKLFAYPYLDQLLAALRHNTIAFVVIMTFQTSLGLLFAYAMFRVK
                     RGLRFYRVVVFLPVIFSLVVVGYLWQSLLDPYYGPINQIITGIGLPEFAKPWLGDTST
                     ALPTLMFINLWRWVGFPAIVFLAGLNAINSEYIEAARIDGASEGQIFRKIIFPLLAPS
                     LTIITVLTFIGAYEWFDLPFVIGGSNGSPAGATDTLALMFYRLSFGSIDSGANNVGVG
                     SALGVLIFTLVGLGAAFGSALLRRREVEQ"
     gene            3859..4686
                     /locus_tag="PLW53_05535"
     CDS             3859..4686
                     /locus_tag="PLW53_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRD36916.1"
                     /translation="MIKLLASRTFFQSVLIINSLIVLLPVFFLINSSLRESNEFARTP
                     FALARDPFWENYQRVWSEGEFGSYFINSLIITGGSLILILILALGAGFVLGRYSFKGN
                     NIIFAFILTGMLVPAKLAILPLFIQLKWMNLLDSRLGLILVYTSAALPAAIFIMSGFF
                     KSLPTDLDNAARIDGASEFKLLSKVLVPLVRPGMAIVAIYSAIPIWNDFFLPLVFLQS
                     PEKKTLMQGLTVFFGQYASQWGVLFAGLCLAALPLVVLYLILSEQFIKGLTAGAVKG"
     gene            4691..5554
                     /locus_tag="PLW53_05540"
     CDS             4691..5554
                     /locus_tag="PLW53_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRD36917.1"
                     /translation="MLSLGIDGGSSSAKWTLIDQTGEIKSKGSSNPIDGHLYRVESQE
                     KMVKFLSELKRELGTLKPDCITIGITGLGSVEKLEEYFLSAFPGSKLSIGTDVALAYR
                     SEFKNGEGIYLYAGTGSIAVHITETGEEISLGGWGYLLGDEGAGYWIGRAALRHLMTQ
                     VEELSSLDELSTAISQTIDGSDWPTIRQFVYSKDRSAIAGLTPIVANCASNGSRSAAK
                     ILDEAASHLAELALRMERRLKSVNMPIAFGGGISSPALGIGKAIKSKLGREIQINSHD
                     HSLTAAKLGLN"
     gene            complement(5551..6906)
                     /locus_tag="PLW53_05545"
     CDS             complement(5551..6906)
                     /locus_tag="PLW53_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD36918.1"
                     /translation="MSVSKALRAALGGDCAVIDDEQILKSYERDQAPFAPSAKAAAVL
                     IAKTIDEVSKAVKFANQESIPVVIRGAGSGLAGGANSIQGCLVISLEKLNRIIEIDQV
                     NQIARVEAGVINSDLDKAAEEFGLTYLPDPASRSWSTIGGNIATNAGGMCCVKYGVTA
                     QHVRAIKVVLSDGEIVEFGFPTKKAVTTLDLLHLFIGSEGTLGVVVEATLSLVPRLKK
                     PVTLIATFPSITKATDAAIEMLIVRPSMLELIDKTTLSAVEEWKPMGFENCEAVLIMQ
                     SDESAIDCQRAQEIALKHGALDAIYSDDPKDSDDLIQVRKLAYSSLERLGVALLDDVA
                     VPVSKISQLVSGVESIAKKHSVKVGVFGHAGDGNMHPTIIYPHNDKEAEARALKAFNE
                     IIALAQSLGGTASGEHGIGGLKLSALINETPERILRIQQEIKAILDPNKIFNPGKKFP
                     L"
BASE COUNT         1934 a         1462 c         1521 g         2091 t
ORIGIN      
        1 gaggcgacac ggcgctacga actggaattg aaggtgctga ggacgttcta gaaggcgcca
       61 ttgaagattt aaagaagttg aatttatcaa aagaagattc tttagtcgga attgctgcaa
      121 gtggacgaac cccctacgtg gttggcgcat tgcaatatgc acagaaggta ggagctgctt
      181 ctattgccct ttcttgtaat ttaaattccg aactttccaa gtttgctgat gtcgcaattg
      241 aggttgatac tggggctgaa attttggctg gctcaacgag attaaaagcc ggaactgctc
      301 aaaagttagt acttaacatg atttcaactg tttcaatgat taatctggga aagacttatg
      361 gaaatcttat ggtcgatctt caggttagta atattaagtt aagggaccgc gcaataagaa
      421 ttattcaatc tgccacgaat tgcgatacta gaagagctca agaagctctt gaagaatcaa
      481 aagatcaagt caaggtggca atcgttatga ttttgttaga tacctctgct gaaaaggctc
      541 ttgagttact aaaagcatca ggctcacgag ttagagccgc cctaggtaag cctgcaaata
      601 cttgaccttt ttagcctaag gcgataattt tcaccaacaa gagtatgtgc tgaaatgatt
      661 tttcacggaa ggtggttatg gctaagcagt tatcgctagt cactcctagc catcattcaa
      721 atttattaga gttaattcag agccaattgg gctcatttca cgccagtgag attgctatcg
      781 cggaagtgat tctgaaagac ccacagttaa ttgcgccaat gaatatcaca caattggcga
      841 ctcacagcaa aacctcggtt tcatcagtag ttagatttag caaggcctta ggttttcgcg
      901 gttttccaga atttaagatg gcgctggtca gtgatctttc ttggaagagc gctaaacaat
      961 ttgataccga atatctagat ggtggaatta cgccaagtga tagtttggca tcaattattg
     1021 aaaaggtcac atactctgat gcaagggcaa ttgaatcaac tgccgagaag ttagatatcc
     1081 aaacatttaa gaaagttgtc gagatttttg aaaaagcaaa gaccatagga ttgtttggat
     1141 tagtctctag tggatttgta gctatggact ttcaatcaaa acttaatcgt ctatccaaga
     1201 gagcaatttc ttggactgac tttcactctg ctatgacctc aattgctgtt ctagataaag
     1261 gtgatgtctt agtagcaata acgcactcag gaactaccgt tgataccgtc gatgttatcc
     1321 gagaatttaa agcacgtggt gtgacaatcg ttttgataac taatactttg agaagtcctg
     1381 ctgcatctct tgcagatttt gtattacata ctgccgcacg ggaaaccacc ttccgtagcg
     1441 gtgcaacagc ttcgcgtatt gcgcagttga cggtggttga ctgtctctgt gtcgcactgg
     1501 cacagagaaa ctggagcggc accaagtctg ctctagatct ttcaagagct gctgtttcaa
     1561 ttcgctccgg aaagaaccac acctaccgcc ctcgaggagg aaattaagtg aaaatctcaa
     1621 aaatccgcgg cttggtcgca gccctttttg cggcagtgac cgtggttgca cctggagctt
     1681 ctatagcttc ggccaacaca gaaattacat tctggagctg gcgcaccgaa gataaggcgt
     1741 tctatgagag ccagatgagg cttttcgaag ctaagaatcc tggtatcaca gttaagttca
     1801 cgccatatct aaataccgag tacaacgcca ttgttgctac tgcactaact gctggaaagg
     1861 gtcctgatgt tcttcagatg cgcccttacg gtggaatggc aaatctctct gatgctggaa
     1921 acttcatgcc gctaacaaca gctgaagttc ctgccttgaa gaagttacca gccggaattc
     1981 ttgctggtgc tcaaggatat aaagataaga agcaatacgg attgccatat gcagtctctg
     2041 cattgggagt tatgtataac ccagacattc tagaaaaagc tggcgttcgc gcacttccac
     2101 aaacttggga gcaaatgcta agtgcatttg ataaagttaa gaaagctgga ttccttccaa
     2161 taggtaatgc cggtggtaat ggacctgctc ttgagcagct acatgcaact gttggtccaa
     2221 ccttctatgg cggaactgca ttcttcaatg atgttgttgc cggaaagaag acatttaccg
     2281 ataaaggata tgtagcaagc ctcaaggctg ttgcagatct cgctaagtgg atgccagcta
     2341 attaccaagg tatcgactac aacactgctc gtggtttgtt tgcaaacgag aaggcagcct
     2401 tctacattgg aggaaattat gagatcggtt acttccgctc tctaaatcct tcaataacaa
     2461 ttggttggtt cccagctcct aaggcaaatg ctggagcaaa gcgttatgtc ggaacttggg
     2521 cagatggtgc ttatgccatt aacgccaaaa ccacacagaa agcagccgct ttgaagctca
     2581 ttaacttcct tggaaccaag gagtttggtc aagctcttgt tgatgaaatt gcattcatcc
     2641 caactgctcc ttatgtgaag attaatgatc cagctgttaa ggaaatcaat cgttggagaa
     2701 caactcttgg aacaccattc ctaaatgttg ttggcttccg atttgaaaac ccaaccagct
     2761 catcaattct tcagcctgga ttccagaagt taattgttgg tggaacgaca ccagaacagt
     2821 tagctaagga tgttcagact gcagtagctt cttggtacgc acctcaaaag ggtaaatcct
     2881 aagaaaagtt agtagctctg ggcgcatcca tggtgcgccc agagcgctat ttctaggtct
     2941 ttaaatgtca tctgcgctgc gaagagaaca gaatcgaacc acaggaacgc ttctaattcc
     3001 tgctctcgca gtgataataa tttttactac tgtcccagcg ctaaccacct taagttatgc
     3061 tttctttagt tgggatggtt ttcagagaag ttcatttgcc gggtttgaga actttcagaa
     3121 actctttgcc tatccatatc ttgaccagct cttagctgct ttaaggcata acacaatcgc
     3181 ctttgtagtc attatgactt ttcaaacatc tcttggactt ctctttgctt atgcaatgtt
     3241 tagggtaaag agaggccttc gtttttatcg cgtcgtggta tttctcccag taattttttc
     3301 actagttgtc gttgggtatc tatggcaatc gttacttgat ccttactacg gaccgattaa
     3361 tcaaataatt actggtattg gattgccaga gtttgccaag ccttggctcg gagatacatc
     3421 gacagcgctc ccaaccttga tgtttattaa tttatggcgc tgggttggat ttccagcgat
     3481 agtattttta gctgggctaa atgcaataaa tagcgaatat attgaagctg cacgtataga
     3541 tggagcctca gagggtcaaa tatttagaaa aatcatcttt ccactacttg ccccttcgct
     3601 aacgataatt acagtgctta cattcattgg cgcatatgag tggtttgacc tcccctttgt
     3661 cataggagga tcaaatggaa gccctgcagg ggcaacagat actttggcgt taatgtttta
     3721 tcgactctca ttcggctcaa ttgattctgg agcaaataat gtaggagttg gttcagccct
     3781 tggagtcttg attttcacac ttgttggcct tggagcagca tttggctcag cgctacttcg
     3841 aagacgtgag gttgagcagt gattaaactc cttgcatctc gtactttctt tcagagtgtt
     3901 ttaataatta attctttaat tgtcttacta ccagtatttt tcctaatcaa ttcatctctt
     3961 agagagtcta atgaatttgc ccgaacccca ttcgctcttg ctagagatcc tttctgggag
     4021 aattatcaga gagtttggag cgaaggggaa tttggttctt acttcattaa ctcattgata
     4081 attactggtg gttcactaat tctaattctt attcttgctt tgggggcagg gtttgttctt
     4141 ggtagatatt catttaaagg taacaatatt atttttgcct ttatcctcac tggaatgtta
     4201 gtccccgcaa agctggcaat cctgccccta tttattcaac ttaagtggat gaatctattg
     4261 gattcaagat tgggattaat tctggtttat acctcagcag ccctgcccgc tgcgattttc
     4321 attatgagcg gtttctttaa atcgcttccg acagatttag ataatgctgc aagaattgat
     4381 ggagctagtg aattcaaatt actttcaaag gtattggttc cgttagttcg ccctggaatg
     4441 gctattgtgg ctatttattc agctattcct atttggaatg acttctttct tcctcttgtt
     4501 tttctgcaaa gcccagagaa gaagacattg atgcaggggt taaccgtatt ttttggccaa
     4561 tatgcctcac agtggggggt tttatttgct gggctctgct tagcagccct tcctttagtg
     4621 gtgctctatt tgatactttc cgaacagttt ataaagggtc ttactgccgg cgccgtgaaa
     4681 ggctaattaa atgctcagtc tgggaattga tgggggttca tcttcagcta agtggaccct
     4741 gatagatcaa acaggtgaga taaagtccaa aggctcaagc aatcccatag atggtcatct
     4801 ctatcgagtt gaatcacaag agaagatggt taagttttta tcagaactaa aaagagaatt
     4861 agggacccta aagcctgatt gcataactat tggaataaca gggttgggct ctgttgaaaa
     4921 gttagaagaa tatttcctaa gcgcctttcc tggttccaag ttatcaatag gaactgatgt
     4981 agcacttgcc tatcgatctg agtttaaaaa tggcgaagga atttatctat atgctggaac
     5041 tggatctatc gcagttcaca tcaccgaaac aggtgaagaa atcagtcttg gtggctgggg
     5101 atatttgctt ggagatgaag gagcgggtta ttggattggt agagcagcac ttcgtcactt
     5161 aatgactcaa gttgaagaac tctcatcatt agatgagcta tcaactgcga tatcgcaaac
     5221 aattgacggt agtgattggc caacgattag acaatttgtt tactcaaaag atagatcagc
     5281 tattgctggc ttaactccaa ttgttgcaaa ttgtgcatca aatggctcta gaagcgctgc
     5341 taagatatta gacgaggcag caagccatct agctgaactt gcgctaagaa tggagagaag
     5401 gctaaagagt gtaaatatgc caattgcttt tggcggtgga atttcaagtc ccgcacttgg
     5461 tattggcaaa gcgataaagt caaagttagg cagagaaatt caaatcaatt ctcatgatca
     5521 ctcactgact gctgcaaaac taggcttgaa ctaaagcgga aatttctttc ctggattaaa
     5581 gatcttattt gggtcaagaa ttgctttaat ctcttgctga atccttaaga tacgctcagg
     5641 agtttcatta ataagcgcag atagctttaa tccaccaatc ccatgttcac cacttgctgt
     5701 gccaccaagg ctttgtgcga gcgcgataat ctcattaaat gccttaagtg ctctagcttc
     5761 agcctcttta tcattatgag gatagatgat tgtgggatgc atatttccat cgccggcatg
     5821 accaaagact ccaactttca ctgagtgttt ttttgcaata ctttcaactc cagagacaag
     5881 ttgtgaaatc tttgaaactg gaactgcaac atcatcaagt agagccactc caagtcgctc
     5941 caaggaagag taggccaatt ttctaacctg gattaaatca tctgaatcct taggatcatc
     6001 agagtagata gcatcaagtg cgccatgttt cagcgctatc tcttgagccc tctgacaatc
     6061 tattgctgac tcatcacttt gcattattaa caccgcttca caattttcaa agcccatcgg
     6121 cttccactct tcaaccgcac ttagtgtggt cttatcaatt agctcaagca ttgatggtct
     6181 gactatcaac atttcgattg ccgcatcagt ggcctttgta atacttggaa atgtggcaat
     6241 taaggttact ggcttcttta aacgaggcac taaggagagc gttgcttcaa caacaactcc
     6301 tagcgttcct tcagaaccta tgaagagatg tagtaaatca agggtagtta ctgctttttt
     6361 ggtggggaat ccaaattcaa caatttctcc atctgataag acaaccttta ttgccctaac
     6421 atgctgcgca gtaacgccat attttacgca gcacatcccg ccagcatttg ttgcaatatt
     6481 gccaccaatt gtggaccagg agcggctagc agggtcgggc agataggtaa gtccaaactc
     6541 ttctgcagcc ttatctaaat cgctgtttat tactcctgcc tcaactcttg ctatctgatt
     6601 gacttgatca atctctatga tcctatttag tttttcaagt gagatcacga ggcagccttg
     6661 aattgaatta gctcctcctg caagcccact accagctcct ctaattacga caggaattga
     6721 ttcttgatta gcaaatttaa cagcctttga aacttcatca attgtcttag caatcaatac
     6781 agcagcagcc ttagctgatg gggcaaaggg cgcctgatct cgttcataag atttgagtat
     6841 ctgttcatcg tcaattacag cgcaatctcc accaagtgcg gcgcgaagag ccttagaaac
     6901 actcatggga atactctaat gactctgtaa tactctgcgc tcttatggct cgtaacatca
     6961 tcaatattga aggggtctca aaagcctttg atattcgccc ccttttag
//
LOCUS       DAOROD010000032         6783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_796_length_6783_cov_35.504905, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000032 DAOROD010000000
VERSION     DAOROD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_6783_cov_35.504905"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1995
                     /locus_tag="PLW53_05550"
     CDS             226..1995
                     /locus_tag="PLW53_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD36919.1"
                     /translation="MARNIINIEGVSKAFDIRPLLVDVSLGISEGERIGIVGRNGGGK
                     STLLKILAGLEEPDAGRVTRANWAKLGSLSQVDSAGPGATVRDVVVGVKKVHEWASDA
                     GVREIFAGLFGGYESSIMDRPFASLSGGEKRRVGIAKLLIQSLDIVLLDEPTNHLDVE
                     GVAWLAKHLRKRRELAVVVITHDRWFLDEVSDSIWEVIDGKVESYEGGYSAYVLAKAE
                     RARQAGAEDARRNNLIRKELAWLRRGAPARTSKPKFRVDAANQLISAEPAPRNSAELL
                     NFATNRLGNMVYEIHNAKLSIGETTLIEKLNWNIGPADRIAIVGVNGSGKTTLMRTLA
                     GQYRFASGKLQTGITVKAAFLSQHLEELNPTWRVLEAVENVALRVDLAGGREISASQL
                     CERLGFDYEGQQKLVRDLSGGEKRRLQLTRLLMDSPNVLLLDEPTNDFDVETLTALED
                     LLDTYAGVIIVISHDRYFLERVCDRFVGLLGNETLQDLALGIEQYLELRAEMISRSVV
                     SEDRKEISGAAQLRLVKKELAKVEKQLEKVVAQEQELIKEQESASFDHQRLLEVGAKL
                     TEIGKIRSELEDKWLELSGQVKE"
     gene            1976..2932
                     /locus_tag="PLW53_05555"
     CDS             1976..2932
                     /locus_tag="PLW53_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD36920.1"
                     /translation="MAKLKSRALRLDGLAALSGVMIALQARANGELSYRLDNAPQAAL
                     VSFSSGLFFITIYAIFSPKIKEGIKHLRSAVRSGEIPKIRLLAGSLGGAFVALQTSVV
                     PLIGVALYSVASIAGQSAVSLLVDRIGLTGGGVKLISPRRIAAAFITVIAVLVSVIDK
                     LEADNFQLFALLLAVIAGALVGVQRALNGQINEHSQNSYTTSLLNFITGTSFLTLFII
                     ILIALGRVELQPLPIGPWWIYTGGVIGVIYIAATSLIVQHLGVLTFTLFSVGGQLIAS
                     LLLDIYSPTQGVSVSWYLVSGIAMTYIGVLVGGVRQSRLERR"
     gene            complement(2908..3513)
                     /locus_tag="PLW53_05560"
     CDS             complement(2908..3513)
                     /locus_tag="PLW53_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRD36921.1"
                     /translation="MSIADWVEQYLNSSAPFFIYLALGAIVFLETGVLLAFFLPGDSI
                     LFAAGLVAATRDDTNILFLITVIFIAAFMGDQVGYVLGRKYGRSYLERRSSKRIDAMV
                     VRAEAFYEKYGWSAVILARFYPWIRTFMPPIAGIAKMNYYKFLAANALGALLWGVGIT
                     LLGFYAASIPLLKDSSRGIAIFFVLLTIGLTLKNYLRSKRD"
     gene            complement(3515..4891)
                     /locus_tag="PLW53_05565"
     CDS             complement(3515..4891)
                     /locus_tag="PLW53_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRD36922.1"
                     /translation="MANTSPGYGITIRVEGRAEFQPVAEITTIISREGAMVTALDVVE
                     SQIDGVVIDVTCDAIDVAHGDRITAALSASPILNVKKVSDRTFLLHLGGKLEVNSKVP
                     LKTRDDLSRAYTPGVARICLAIAKDPSDARRLTIKRNTVAVVTDGSAVLGLGNLGPAA
                     ALPVMEGKAALFKRFADVDAWPVCLDTQDVDEIVRTVQLIAPVYGGINLEDISAPRCF
                     EIEARLRELLDIPVFHDDQHGTAVVVLAALKNALKLVKKDLGKTKIILNGAGAAGTAI
                     ARLLVLAGARNIIGFDSSGVISKKSASNNAMRKWFIDNCNPEQFEGNLSQAMSGADIF
                     IGVSAPNLLSEADIQSMAKDAIVFALANPDPEIDPVLARKHAAVVATGRSDQPNQINN
                     VLAFPGIFRGLLDAGASKITDELLVACAEAIASCVSESQLNASFIVPSVFDANVVKSV
                     ADAVKRFT"
     gene            complement(4892..5530)
                     /gene="upp"
                     /locus_tag="PLW53_05570"
     CDS             complement(4892..5530)
                     /gene="upp"
                     /locus_tag="PLW53_05570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRD36923.1"
                     /translation="MKVVVVNHPLVTHKLTVLRDKQTNSQIFRHLVEELVTLLAYEAT
                     RDVKIQEVSIETPVTKTTGAMLATPRPVVVPILRAGLGMLEGMMRVIPSADVGFLGMV
                     RDEKTLQASTYANRLPDDLTKRQCYVLDPMLATGGTLIAAINYLIERGAESVTAICLL
                     SAPEGAKAVEEAFKDSKTPIAIVTGAMDEKLNEHGYIVPGLGDAGDRLYGVV"
     gene            5603..6076
                     /locus_tag="PLW53_05575"
     CDS             5603..6076
                     /locus_tag="PLW53_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HRD36924.1"
                     /translation="MADETLEFALLSWRDDGQWRISQLPDEATSDIGLVLDALRAQQV
                     DGGAIALLAIDDQFFIIIRQIGAKMQMVLSDVMAALDYEIAAEVLELSDIEFPAEDDA
                     DEPAGDLNIFADLGLDAMELQMICDDDELYPEEQLDVIARRIGFGDIYSEVIENL"
     gene            6077..6529
                     /gene="tadA"
                     /locus_tag="PLW53_05580"
     CDS             6077..6529
                     /gene="tadA"
                     /locus_tag="PLW53_05580"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRD36925.1"
                     /translation="MNHERAMRVALERAKFGSTSSGDVPVGAVMLNKDGEIVALGNNQ
                     RELLKDPLGHAEIVAIKAASRSIGAWRLDECTLVVTLEPCVMCAGAIMAARIPRLVFG
                     AWDEKAGACGSVWDLIRDPRALHKVEVISGVLEQECAQLLTQFFAQKR"
     gene            6568..6657
                     /locus_tag="PLW53_05585"
     tRNA            6568..6657
                     /locus_tag="PLW53_05585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6602..6604,aa:Ser,seq:cga)
BASE COUNT         1926 a         1453 c         1563 g         1841 t
ORIGIN      
        1 aaaccataaa gttatagggc cagatgattc caaatacata gcttggcggc tgccagggcg
       61 gcctatttag cgatgagtac catccgggtg aggaagagac ccagattcct gaaccaaagg
      121 cgtagataag aactataaga attccagtac cagctgcaat caacttaaaa tccactagag
      181 gactgtatcg aagtagggtt cacactttct ggctaacctt gcgcaatggc tcgcaacatc
      241 atcaatattg aaggggtctc aaaagccttt gatattcgcc cccttttagt tgatgtctca
      301 ctaggaatct ctgaaggaga acgcatcggc attgttggtc gaaatggtgg tggcaaatca
      361 acacttctta aaatcctggc aggccttgaa gagcctgatg ctggacgagt cacaagagcc
      421 aactgggcaa agcttggctc actaagccag gttgatagcg cagggccagg tgcaacagtt
      481 cgagatgttg tggttggagt taagaaagtt catgagtggg caagtgatgc tggagtgcga
      541 gagatatttg cagggctctt tggtggatat gaaagctcaa ttatggatcg cccctttgcc
      601 tcactatcag gaggtgagaa gcgaagagtt ggtattgcaa agctacttat tcagagccta
      661 gatattgtct tacttgatga accaacaaat catcttgatg ttgaaggagt ggcctggctt
      721 gctaaacatc ttcgcaagcg ccgcgaactt gctgttgtcg taattaccca tgatcgttgg
      781 tttttggatg aagtatcaga ttccatctgg gaggtaatag atggaaaagt tgaatcatat
      841 gaaggtggtt actctgccta tgttctagcc aaagctgaga gagcaagaca ggctggagca
      901 gaggatgcaa gacgaaataa cttaattcgc aaagaacttg cctggctgcg cagaggcgcg
      961 cctgcaagaa cttcaaagcc aaagtttcga gtcgatgctg ccaatcaatt aatctcagca
     1021 gagcctgcgc caagaaatag cgcagagtta ttaaattttg ccaccaaccg attaggcaac
     1081 atggtctatg agattcataa cgccaagtta agtatcggtg aaacaacact gattgaaaaa
     1141 ctcaactgga atattggccc tgcagatcgc attgcaatag tgggcgttaa cggctcagga
     1201 aaaacaacct taatgcgaac cctagccggg caatatcgct tcgcctcagg caaactgcag
     1261 actggaataa cagtaaaagc tgcttttcta tctcagcact tagaggagtt aaatccaacc
     1321 tggcgagtat tagaggcggt tgaaaatgtt gctctgcgag tagatcttgc tggagggcgt
     1381 gagatttcag cttcacaact ctgtgaacgc cttggctttg attatgaggg gcaacagaag
     1441 ttggtccgcg atttatctgg aggtgaaaaa cgaaggctgc agttaactcg cctacttatg
     1501 gattcaccaa atgtgctctt actagatgag ccaacaaatg attttgatgt tgaaacctta
     1561 actgctctag aagatctttt agatacttat gctggtgtaa taatagttat ctcccacgat
     1621 cgctatttcc ttgaaagagt ttgtgatcgt tttgttggac tacttggcaa tgaaacgctt
     1681 caagacttgg ctttaggaat tgaacaatat ttagagctaa gagctgagat gatttcaaga
     1741 agtgttgtta gcgaggatag gaaagagatt tcaggggctg cgcaattgcg tctagttaag
     1801 aaagaattag caaaagttga gaaacagtta gagaaagttg ttgctcaaga gcaggagtta
     1861 ataaaggagc aagagagcgc ttcctttgac catcagagac ttctggaagt tggcgctaaa
     1921 cttaccgaga taggaaaaat tcgatctgaa ctagaagata agtggttgga gttaagtggc
     1981 caagttaaag agtagagctc tgcggctaga tggacttgct gccctctctg gggtaatgat
     2041 tgcccttcag gcaagagcca atggcgagct ctcttatcgt ctagataacg cgcctcaagc
     2101 ggcgctcgtt tcattttctt caggactttt ctttataact atctatgcaa tttttagtcc
     2161 taaaattaaa gagggcataa agcaccttag aagcgcagtt agaagtggag aaattccaaa
     2221 gataagactc cttgcaggat cactaggcgg ggcatttgta gcgcttcaaa ccagcgttgt
     2281 tcctttaatt ggcgtggctc tctactctgt tgcatcaatc gcaggtcaga gcgcagtatc
     2341 gcttttagtg gatcgaatag gtttaactgg aggtggggta aaactcatct caccaaggcg
     2401 aattgcagcg gcgttcatta ccgttatcgc agtattagtc tcagtgatag ataagttgga
     2461 ggctgataat tttcaactct ttgcgctcct tcttgctgta atagctggcg ctttagttgg
     2521 ggtgcagcga gcgttaaatg gtcagattaa tgagcattca caaaatagct atacaacttc
     2581 acttctcaac ttcataacag gaacatcctt cttaactcta ttcataatta ttctgatcgc
     2641 ccttggaaga gttgaactac aaccacttcc aataggtcct tggtggatct ataccggcgg
     2701 ggtaattggg gttatctata ttgcagcgac ctctttgatt gtccaacact taggagttct
     2761 aacctttact ctctttagcg ttggtggcca gttaattgct tcattacttt tagatattta
     2821 ctctccaacc caaggagtaa gcgttagttg gtatttagta tctgggattg caatgactta
     2881 tatcggagtg ttagttggtg gagtccgtca gtcgcgctta gagcgcaggt aattctttag
     2941 cgttaagcca attgttagca atacaaagaa aattgcaatg cccctggatg agtcctttag
     3001 taatggaatt gaagcagcat aaaaaccaag gagtgttata cctactcccc agagcagagc
     3061 ccctagagca tttgctgcta agaacttgta gtaattcatc ttggcgattc cagcaatagg
     3121 cggcataaag gtgcgaatcc agggatagaa gcgagccaga atgactgcgc tccagccata
     3181 tttttcatag aaggcttcag cgcgcactac catcgcatca attcgtttcg aagatcgtct
     3241 ctctagataa cttcgcccat attttcgacc caatacataa cccacctgat ctcccataaa
     3301 tgctgcaata aagatcacgg tgattagaaa taaaatattt gtgtcatctc tcgtcgcagc
     3361 caccaaacct gcagcaaaga ggattgaatc tccaggcagg aagaaggcta aaagcacgcc
     3421 agtttcaaga aagacaattg cccctaaagc aaggtaaata aagaatgggg cagaggagtt
     3481 taaatactgc tccacccaat cagcaatcga caatttaagt aaacctcttt actgcatctg
     3541 caacagactt aacgacattt gcatcaaaaa cactggggac aatgaaggag gcatttaatt
     3601 ggctctcact tacacaactt gcaattgctt cagcgcaggc aacaagtagc tcatcggtaa
     3661 tcttcgaggc tccagcatct aatagcccgc gaaatattcc aggaaatgcc aaaacattat
     3721 tgatttgatt tggttgatca cttcttcctg tggcaacaac agctgcatgt tttctagcca
     3781 agactgggtc aatttcagga tctgggtttg ccagcgcaaa aacaatagcg tccttagcca
     3841 ttgattggat atcagcttca cttaagagat ttggcgcact aactccaatg aaaatgtcag
     3901 ccccactcat agcttggctg agatttccct caaattgctc tgggttgcag ttatcaataa
     3961 accacttgcg catagcgtta ttactggcac tctttttact aataacccca ctgctatcaa
     4021 agccaatgat gtttctagct cctgcaagga caagcaggcg agcaattgca gttccggcag
     4081 ctcctgcgcc attgagaata attttagttt tgccaagatc tttcttaact agctttaaag
     4141 cattttttag ggcagctaga acaacaactg ctgtgccatg ttgatcatca tggaaaactg
     4201 gaatatctag gagttctcta aggcgcgctt caatctcaaa acatcttgga gctgagatat
     4261 cttctagatt aattccgcca tagaccgggg cgattaactg aacagttctt acaatttcat
     4321 caacatcttg agtatcaaga cagactggcc aagcatcaac atcagcaaat ctcttaaaga
     4381 gcgcagcttt tccttccata actggaagag ctgctgcagg gcctagattt ccaaggccca
     4441 aaactgccga accatcggtg actacagcga cggtattgcg cttaattgtg agccgtctgg
     4501 catcagatgg gtctttagca atggctagac agattcttgc aacccctggg gtataggccc
     4561 tggatagatc atcacgagtt ttgagtggaa cctttgaatt aacttcaagt tttccaccaa
     4621 ggtggagcaa aaatgttcga tcagagactt tctttacatt aagaatcggt gaagcactta
     4681 gcgctgctgt aattctatct ccgtgagcaa catctatcgc atcacaagtt acatcaataa
     4741 ctacgccatc tatttgtgat tcaacaacat caagtgcagt aaccatcgca ccttctcgag
     4801 aaataattgt ggttatctcg gcaacaggtt gaaattcagc gcgtccttca acacgaatag
     4861 ttattccata tcctggggag gtattagcca tttagacaac tccgtagagt cgatctccag
     4921 catcgccaag gccaggaacg atatagccat gctcgtttaa cttctcatcc attgcgccag
     4981 taacaatagc tattggagtc ttagaatctt taaatgcctc ttccactgcc tttgctccct
     5041 caggcgctga gagtaaacag atagcggtaa cagattctgc ccctcgctca attagataat
     5101 taatggcagc aattagagtt ccaccagtag caagcattgg atctaggaca taacactgac
     5161 gctttgttaa atcatctggc aatcgattgg cataggttga tgcttgaaga gtcttttcat
     5221 ctctaaccat tcctaaaaat cccacatcag cgctaggaat cacgcgcatc attccctcga
     5281 gcattccaag gcctgctcta agaattggaa ctactacagg cctaggtgtt gccagcattg
     5341 cgcctgttgt cttggtaact ggagtctcaa tcgagacctc ttggattttt acatccctag
     5401 ttgcttcata tgccaaaagg gtgactagct cctcaaccaa gtgcctaaag atttgtgaat
     5461 tagtctgctt atcgcgtagc accgtcaatt tatgggtcac aagcgggtga tttactacaa
     5521 cgaccttcat ggcggcactt tactaccaag ggcaacggtg gaactatgag gcattgcatt
     5581 tcttatgagc gggaggtgtg agatggcaga cgaaactctt gaatttgctc tcctttcctg
     5641 gcgtgatgat gggcaatggc gaatctctca attaccagat gaagcaacta gcgatatcgg
     5701 tctagtcctt gacgccctta gagcacaaca agtagatggc ggagcaattg cacttcttgc
     5761 tatcgatgat caatttttca taatcatccg ccagattggc gcaaagatgc agatggtttt
     5821 aagtgatgtc atggcagcac ttgattatga aattgctgca gaggttttag agttaagcga
     5881 tattgaattt ccagcagaag atgatgctga tgaaccagca ggggacttaa atatatttgc
     5941 agatcttggc cttgatgcca tggagctcca gatgatctgt gatgatgacg agttatatcc
     6001 agaggagcag ttagatgtaa ttgctagaag aattggcttt ggcgatatct atagtgaagt
     6061 tattgagaat ctttaaatga accatgaacg cgcaatgcgc gtagcacttg agcgcgcaaa
     6121 atttggaagt acttcaagcg gcgatgtgcc agttggcgca gtaatgctca ataaagatgg
     6181 cgagatagtt gctcttggaa ataatcagcg agaattactt aaagacccgc taggtcatgc
     6241 tgaaattgtt gctatcaaag cagcttcaag atcaattggc gcctggcgct tagatgagtg
     6301 cacgctagtt gtcactcttg aaccatgtgt gatgtgtgcg ggggcgataa tggcagcaag
     6361 aattcctagg ttagtttttg gcgcctggga tgaaaaggca ggagcttgcg gctcagtttg
     6421 ggatttgatt cgcgatccaa gagcgctaca taaagttgaa gttatctcag gtgttttaga
     6481 gcaagagtgt gcccaattat tgacgcagtt ttttgctcaa aagaggtaat ttaaaaactg
     6541 attaggccct cgcgtatagt cacctacggt ggcgtgtccg agcggcctaa ggagcgcgcc
     6601 tcgaaagcgc gtgttgggga aacccaaccg tgggttcaaa tcccaccgcc accgccaact
     6661 atttaactct taaaaacctc tttaaacccc agatagttac cagaaacatc gcttctctca
     6721 caatattttt tgacatcttg ctcacgccat tttcgcgctc tctaaaagtt atcggaactt
     6781 caa
//
LOCUS       DAOROD010000033         6435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_863_length_6435_cov_21.965987, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000033 DAOROD010000000
VERSION     DAOROD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_6435_cov_21.965987"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..906
                     /gene="nth"
                     /locus_tag="PLW53_05590"
     CDS             205..906
                     /gene="nth"
                     /locus_tag="PLW53_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRD36926.1"
                     /translation="MAESFVAKKKRAGVMYRVLSKSYPDVKCELDFNNPLQLLIATVL
                     SAQCTDKRVNSVTPALFKKYRKVEDFAGANLSELQRIIKSTGFFRAKARSIKGLANKI
                     VKDYKGQVPETLDELVTLPGVGRKTANVVLGHAFNTPGLTVDTHFGRLVRRFGWSKEM
                     DPVKVEFAIMKLIPQKEWTNLSQRLIWHGRRVCHSRKPACGACALAKLCPSYGIGEVD
                     LEKAKKLLKSDKDFR"
     gene            903..1439
                     /locus_tag="PLW53_05595"
     CDS             903..1439
                     /locus_tag="PLW53_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRD36927.1"
                     /translation="MRIWLVVFLLLLTSCSTQSDKSATPVDSLAPCSSIKTTASSSEG
                     MLLECLDSDEEISIKSIAGPAVISVWASWCTNCAAQRKNFIKLHQEAGDLLQVVGVDV
                     EERAKDDGYNHALENGLAFPQLYDPDGRSSNYFGPGVPITQFINKDGVLTFQKVGPIF
                     TYEEMQELVKEYLEIDIG"
     gene            1436..1936
                     /locus_tag="PLW53_05600"
     CDS             1436..1936
                     /locus_tag="PLW53_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRD36928.1"
                     /translation="MILDIALVVIALIAIATGYNKGALATLLSILGYFGGGVLGFGLA
                     NWYSAEWKGLTAIIFLHLFAIFAGANLGRWILEKSGIGIHKRILFGPFKFINSILGAA
                     LSLAQVALIAYVVITLIKYLPWQLPDSIIGSSDIYREIADFNLLSFQISDLLKSTSLR
                     WDQLRS"
     gene            complement(1863..3809)
                     /gene="acs"
                     /locus_tag="PLW53_05605"
     CDS             complement(1863..3809)
                     /gene="acs"
                     /locus_tag="PLW53_05605"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRD36929.1"
                     /translation="MSEALSNLGHENRTFPPTKEFASQANAKQEIYERADKDYLAFWE
                     EQANNLYWHKKWDQVLDWQSPFAKWFVGGKLNASYNALDRHVLEGRGDRIAFLFEGEP
                     GDSKQITYSQLLTEVKKCANALTSLGIKSGDRVAIYMPMIPEAAIAMLACARVGAAHS
                     VVFGGFSADSLLARIQDADASLVITADGGFRKGSAFALKSIVDEALKGETNVKNVLVV
                     KRTGQDVSFTDRDIWWHELVDKQSSEHEAEAFDSEHPLFILYTSGTTAKPKGIAHTTG
                     GYLTQAAFTHKMVFDLKPEKDIYWCTADVGWITGHSYVVYGPLINGATQVIYEGTPDT
                     PHKGRIFEIIEKYKVTILYTAPTLIRTWMKWGDEHPNRFNLKSLRLLGSVGEPINPEA
                     WIWYRKVIGSDNCPIVDTWWQTETGAIMISPLPGITATKPGSAMRSLPGISVKVIDNE
                     AKEVGNGGGGYLVIDRPWPSMLRGIWGEPERYKQTYWSRFDGLYFAGDGAKLDDDGAL
                     WLLGRVDDVMNVSGHRISTTEVESALVSHPSIAEAAVVGAKDEITGQAIVAFVILRGG
                     VAADKDLEQSLKSHVTKEIGAIARPRQIMVVLELPKTRSGKIMRRLLQDVAENRAVGD
                     ATTLADPNVMKLISEGLKSGTKED"
     gene            3941..4078
                     /locus_tag="PLW53_05610"
     CDS             3941..4078
                     /locus_tag="PLW53_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36930.1"
                     /translation="MYLLTPAMGLFSTNIYDFLLRVMGSEILRTIASFIIRSALTRIG
                     L"
     gene            4165..5451
                     /locus_tag="PLW53_05615"
     CDS             4165..5451
                     /locus_tag="PLW53_05615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRD36931.1"
                     /translation="MPAIVLLGAQWGDEGKGKATDLLGDRVKYVVRYQGGNNAGHTVV
                     IGDQKYALHLLPSGILTPNCIPVIGNGVVIDPAVLLEEIAGLNERGVDTSNLKISTNA
                     HLITPYHRTIDKVSERFLGKSKIGTTGRGIGPAYADKINRIGIRVQDLFDTSILRQKI
                     EGALRDKNQVLIKVFNRKGLEVDEVLNEYLQYAEILKPYVTDTSLLLNQALDRAENVL
                     LEGSQGTLLDVDHGTYPFVTSSNPTAGGASTGSGIGPTRISRVIGIVKAYTTRVGSGP
                     FPTELFDEDGEKLRSIGGEFGTTTGRSRRCGWYDAPIARYAVRINGITDFFLTKLDVL
                     TGWEKIPVCVAYEIDGKRVEELPASQSDFHHAKPIYEYLPGWSEDISKAREISDLPKN
                     AQSYIKFLEEISGAPMSAIGVGPGRDETIAVRDLIS"
     gene            5448..6407
                     /gene="galE"
                     /locus_tag="PLW53_05620"
     CDS             5448..6407
                     /gene="galE"
                     /locus_tag="PLW53_05620"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRD36932.1"
                     /translation="MSKVLVTGGAGYIGAHVAAELLKSGYSIRIYDDFSNGLHRRVDG
                     KFRDIVDGDMLDRVKLLAALDGIDAVIHLAAKKAVEESVKDPLKYYENNVGGTLNLLG
                     AMAAKGVKQLVYSSSAAVYSPNDKEAVVEDDPTEPLSPYGASKLLSEQLISSVGSAEQ
                     ISNISLRYFNVVGSNIAEFGDNSKDNLVPKVFLALKNGKRPQIYGSDYPTPDGTCIRD
                     YIHVQDLALAHLAALKKVESGYISQVYNVGSGKGYSVKEMMDQISKSIGRDINPELSP
                     ARVGDSPKLIASIDKIKEQLGWSPKASLEEMIDSAWQAEVART"
BASE COUNT         1842 a         1375 c         1395 g         1823 t
ORIGIN      
        1 ccctctctaa agagaacctg acctttgcgc agttttacca agttcattga ggctcgaagt
       61 gttgaggcgg cagcttcatc aagaccagaa aagagcgggg ttttacgaac aattacttca
      121 tcgacttggt catttaagcg cggagccata gcgccacctt accttctggt gaaaaagata
      181 ccaattcggg ctatttttaa cgctatggct gagagctttg tagcaaagaa aaagcgagcc
      241 ggggtgatgt atagagtttt atctaagagt tatccagatg ttaagtgtga attagatttt
      301 aataacccac ttcaactcct aattgcaaca gttctttcag cccaatgcac cgataagcga
      361 gttaactcag ttaccccagc tctctttaag aaatatcgaa aggttgaaga ctttgcagga
      421 gcaaatttaa gcgagcttca aagaatcatt aaatcaaccg gcttctttcg agctaaagct
      481 cggagcatta agggccttgc aaataagatt gttaaagatt acaaaggcca agttccagag
      541 acattagatg agttagtaac tcttcctggg gtaggaagaa agacagcaaa tgttgttcta
      601 ggacatgcct ttaatactcc agggctcaca gttgatactc actttggccg tttggtgaga
      661 aggtttggct ggagcaaaga gatggatcct gtgaaggtgg aatttgcaat tatgaagtta
      721 ataccgcaga aagagtggac caatctctct cagcgcttga tctggcatgg caggcgggta
      781 tgtcactcaa ggaaaccagc atgtggggcc tgcgcattgg ctaagttatg ccctagttat
      841 ggaattggcg aagtagattt agaaaaagca aaaaagttac tcaagagcga taaggacttt
      901 agatgagaat atggctcgtt gtttttcttc ttctacttac atcctgctca acacaaagtg
      961 ataagagcgc aactcctgtc gactcacttg ccccatgctc ttcaataaaa actactgcat
     1021 catcaagtga aggaatgctc cttgaatgcc tcgatagcga tgaagagata tcaattaaat
     1081 ctatagcagg acctgctgtt atctcagtct gggctagttg gtgcactaat tgcgcagcgc
     1141 agcggaagaa ctttataaag cttcaccaag aggcagggga tttattacaa gttgtgggag
     1201 tagatgttga agagcgcgct aaagatgatg gctataacca cgcccttgaa aatggtttag
     1261 ctttcccgca gctctatgat ccagatggca gatcatctaa ttactttggc ccaggagttc
     1321 caataactca atttataaat aaagacggag ttttaacgtt ccagaaagtt ggaccaattt
     1381 tcacctatga agagatgcag gagttagtaa aagagtatct agagatagat attggatgat
     1441 cttagatatt gcgctagttg ttatcgctct cattgcaatt gcaactggtt ataacaaggg
     1501 agcacttgct actcttttat caatccttgg atactttggc ggcggcgttt taggctttgg
     1561 ccttgctaat tggtatagcg ctgagtggaa gggtcttact gcaatcatct ttctccatct
     1621 ctttgccata tttgcaggag caaatttagg gcgttggatt ctagaaaaga gtgggatagg
     1681 aattcataaa aggatccttt ttggcccctt taaattcatt aactccatcc ttggagcagc
     1741 tctctcactt gcccaagtag cacttattgc ctatgtggta attactttaa tcaaatacct
     1801 gccttggcaa ctaccagatt caattattgg tagtagcgat atctatagag agattgctga
     1861 ctttaatctt ctttcgttcc agatttcaga ccttctgaaa tcaacttcat tacgttggga
     1921 tcagctaagg tcgtagcatc accaactgct ctattttcag ctacatcttg aagcaggcgg
     1981 cgcatgatct tgccgcttct agtctttgga agctctaaaa caaccatgat ctggcgcggt
     2041 cttgctattg cgccaatttc ctttgttaca tggctcttta ggctctgctc aagatcttta
     2101 tcggctgcaa ctccacctcg aagaattaca aaagcaacta ttgcctggcc agtaatttca
     2161 tcctttgctc caacaactgc tgcttcagca attgatggat gtgaaactag agctgattca
     2221 acctctgttg ttgagattcg atgcccagag acattcataa catcatcaac tcttccaaga
     2281 agccagagcg ctccatcgtc atctagcttt gcgccatctc ctgcaaaata aagtccatca
     2341 aatcttgacc agtaagtctg tttatatcgc tccggctcgc cccagattcc gcgcagcatt
     2401 gatggccaag gtctatcaat tactaagtat ccgccgccgc cattaccaac ttctttagct
     2461 tcgttatcaa taactttcac gctaattcca ggaagtgatc tcattgcaga tcctggcttt
     2521 gttgctgtta ttcctggaag aggcgagatc atgatggctc cagtctcggt ctgccaccag
     2581 gtatcaacaa ttggacagtt atctgagcca attacctttc gataccagat ccatgcctct
     2641 ggatttattg gctcgccaac agagccaaga agtcttaagg attttaagtt aaatctattt
     2701 ggatgctcat ctccccactt catccaagtt ctaataagag ttggcgcggt ataaagaatc
     2761 gtcaccttat atttttcaat aatttcaaag attcgcccct tatgtggagt atctggggtt
     2821 ccttcataga tgacctgtgt tgcgccatta attagcggtc catagaccac ataagaatgg
     2881 ccagtaatcc agccaacatc tgctgtgcac caatagatat ctttctcagg ctttagatca
     2941 aagaccattt tgtgagtaaa ggcggcctgg gtgagatatc ccccggtggt atgggcaata
     3001 cctttaggtt tagcagtagt tcctgaggtg tagagaataa agagcggatg ctcgctatca
     3061 aaagcctcgg cctcatgctc actactttgt ttatcaacta actcatgcca ccagatatct
     3121 cgatccgtaa aagaaacatc ttgcccggtt ctctttacta ccaaaacgtt tttaacgtta
     3181 gtttcaccct ttagagcttc atcaacaatt gatttaaggg caaaggcgct accttttcta
     3241 aaaccaccat ctgcagtaat aactaaactt gcatcagcat cttgaattct tgcaaggagt
     3301 gaatctgcag aaaagccgcc aaagaccacg ctatgggcag cgccaactct tgcgcaggca
     3361 agcattgcaa ttgctgcctc tggaatcatt ggcatataga ttgctactcg atcaccactt
     3421 ttaattccaa gtgatgttag agcgttagcg cactttttaa cttcggtaag aagctggcta
     3481 taggtaatct gcttactatc tccaggttca ccttcaaaga gaaatgcaat tcgatcccca
     3541 cgtccttcta aaacatggcg atcaagtgcg ttatacgaag cgttgagctt tccaccaaca
     3601 aaccactttg caaaagggct ctgccaatct aaaacctgat cccacttctt atgccagtaa
     3661 agattatttg cttgctcttc ccagaatgct aaataatctt tatctgctct ttcatagatt
     3721 tcctgtttag catttgcttg agaggcaaac tcttttgttg gaggaaatgt tctattttca
     3781 tgtcctaggt ttgatagcgc ctcactcaca ttgtcagatt accagcgttg agttttcccc
     3841 actacggcct aatgagagga attatttata aacatattgg gcatattgcc tgccttcgca
     3901 agatgcgaaa aatagaccag aactagaaag caagggcagc atgtatctac taactccagc
     3961 aatgggcctc tttagcacca atatctatga cttcctcctt agagtgatgg gctctgagat
     4021 tttaaggacg atagcctcct tcatcattag aagcgcgcta acccgtatcg ggctatagat
     4081 ggctctggca gctcctagcg atttctccta tctggccaaa tgtgtgaaaa ttacccgtaa
     4141 tcctcatagg taggagaaaa tcgcgtgcca gccattgttt tacttggtgc tcaatggggc
     4201 gatgagggca agggaaaagc cacagatctt ctcggtgatc gagtcaaata tgtagtccgc
     4261 tatcaaggcg gcaacaatgc aggccatacc gttgtaattg gcgatcaaaa atatgcactt
     4321 catcttcttc cttcaggaat tctcacccca aattgcattc cagtaattgg aaatggcgtt
     4381 gttatagatc cagcggtgtt acttgaagag attgctggat taaatgagcg cggggttgat
     4441 acctcaaatc taaagatttc aactaatgct catctaatta ctccttatca ccgcacgata
     4501 gataaggttt ctgagagatt tttaggaaag tcaaagattg gaacaaccgg gcgaggaata
     4561 ggcccagcat atgccgataa aataaatcgc attggaatta gagtccaaga tctctttgat
     4621 acctcaattc ttagacaaaa aattgaaggc gctctaagag ataagaatca ggttttaatc
     4681 aaagtattta atcgcaaagg tttagaagtt gatgaagttt taaacgagta tctccaatat
     4741 gcagagatcc taaaaccata cgttaccgat acctcgcttc tattaaatca agcacttgat
     4801 agggcagaga atgttttact tgaaggctcg cagggaactc ttcttgatgt tgatcatggc
     4861 acctatcctt ttgttacctc atctaatcca actgcaggag gagcatctac tggttctgga
     4921 attggaccaa cccggatctc aagagtgatt ggaattgtta aggcctacac aactcgcgtt
     4981 ggctctggcc cttttccaac tgagctcttt gatgaagatg gcgagaaact acgctcaatt
     5041 ggaggcgaat ttggcactac cactgggcgc tcaagaaggt gcggctggta tgacgctcca
     5101 attgcaagat atgccgtgag aatcaatggc attacagact tcttcttaac caagctagat
     5161 gtgttaactg gctgggagaa gattcctgta tgtgtggcct atgagataga tgggaaaaga
     5221 gtagaagagc ttccagcatc acagagcgat tttcaccatg caaagccaat ttatgaatac
     5281 cttccaggct ggagcgaaga tatctctaaa gcacgcgaga tatccgatct acctaaaaac
     5341 gcacagagct atattaagtt tctagaagaa atttcaggtg ctccgatgag cgctattgga
     5401 gttggacctg ggcgcgatga aactattgca gttagagatt taatctcatg agtaaggttt
     5461 tagttacagg tggtgctggt tatatcggcg cacatgttgc agcagagctt ttaaagagcg
     5521 gctatagcat aagaatctat gatgatttct ctaatggcct tcatagacga gtagatggaa
     5581 aatttagaga tatcgttgat ggagatatgc tcgatagagt aaaactactt gcagcacttg
     5641 atggaattga tgcagtgatt catctagcag ctaagaaagc tgttgaagaa tctgtgaaag
     5701 atcctttgaa atattatgag aacaatgttg gtggcactct aaatcttctt ggcgcaatgg
     5761 ctgcaaaggg agtaaagcag ctggtctatt catctagcgc tgcggtttat tcaccaaatg
     5821 ataaagaagc agttgtagaa gatgatccaa cagagccact ttccccatat ggagcaagca
     5881 agctcctttc tgagcagttg atttcaagtg ttggctcagc tgagcagatc tcaaatatca
     5941 gccttcgtta tttcaatgtt gttggctcaa atatcgctga atttggcgat aattccaaag
     6001 acaatctagt ccctaaagtt tttctagctc taaaaaatgg taagcgcccg cagatctatg
     6061 gcagtgatta tccaacccca gatggcacct gcatccgtga ttacatccat gttcaggatc
     6121 tagcacttgc tcatcttgct gctctaaaaa aagttgagtc aggatatata agtcaggtct
     6181 ataacgttgg ttcaggtaag ggctactcag ttaaagagat gatggatcag atctcaaaga
     6241 gtattggaag agatataaac ccagaactct ctcctgcaag agttggagat tcaccaaagc
     6301 taattgcctc aatagataag attaaagagc aactaggctg gagcccaaag gcgagccttg
     6361 aagagatgat tgattcagcc tggcaggcag aggtggcaag aacatgagca tccttgctaa
     6421 tcgctatgca tcaga
//
LOCUS       DAOROD010000034         6301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_892_length_6301_cov_28.486711, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000034 DAOROD010000000
VERSION     DAOROD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_6301_cov_28.486711"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLW53_05625"
     CDS             <1..226
                     /locus_tag="PLW53_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HRD36933.1"
                     /translation="LSDLEVRILDFERQWWKYAGAKDAAIRELFDLSTRSYYELLNNL
                     IDREDALAASPLLVKRLRRLRQARISARSR"
     gene            379..1998
                     /gene="groL"
                     /locus_tag="PLW53_05630"
     CDS             379..1998
                     /gene="groL"
                     /locus_tag="PLW53_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227359.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRD36934.1"
                     /translation="MAKMIAFNEDARRGLERGMNTLADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPWEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGSNPMALKKGIEKAVEAISTELSAMSKPVETKEQIAATASISAADSTIGEMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISPYMVTDTERMEAVMEDAYV
                     LICNSKISNIKDLVPILEKVMQSGKPLAIIAEDIEGEALATLVVNKIRGTFRSVAVKA
                     PGFGDRRKAMLQDIAILTGGTVISEEVGLKLEAATLDLLGKARKVVVTKDETTIVEGA
                     GDDEMIKGRVNQIRSEIEKTDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVSGGGVALLQASKKAFEKLKLTGDEATGAKIVELAVEAPLK
                     QIAMNAGLEGGVVVEKVRNLAAGHGLNAATGEYVDMIKAGIIDPAKVTRSALQNAASI
                     AALFLTTEAVIADKPEPKSAAPAAPGGDMDF"
     gene            2077..4155
                     /locus_tag="PLW53_05635"
     CDS             2077..4155
                     /locus_tag="PLW53_05635"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRD36935.1"
                     /translation="MEKLDVLSPEFIIDRELSWLDFNTRVLELAEDTSIPLLERVRFL
                     AIFSSNLDDFFMIRVAATKLKIESGASAPNASGLTPVALLEEITQKVSELIKRKVECL
                     HNSIKPGLVKADIHMVHWNELTQAERSYVGEIFDSQIFPVLTPLAVDPSHPFPYISGL
                     SLSLAVIVKHPESGEEFFARVKVPSVIARFISTQKSIKTRFIALEEIIAAHLTELFPG
                     MIIVDHYAFRLTRNEDLDLEEEDSENILETMEQELLRRKFGPPVRLEVDSEIQDDLLD
                     RLISELEITEREVIKYQGPLDLTGLNTLADLDRPDLHFPPFKSQMPTGLVDIEDLGTD
                     ALFNLINQGEILLHHPYDSFSSSVVRFIEAAAIDPKVLAIKQTLYRTSGDSPIMEALI
                     EAAQAGKQVLAVVEIRARFDELANVRWARKLEEAGVHVVYGLMGLKTHAKLSLVIRQE
                     ESGLNRYCHVGTGNYNPKTARLYEDLGLLSADPVLCDDLSRLFNQLSGFVNDIEFNRL
                     IVAPKSLRKSLINKIDREIENAREGKPAHIRFKLNSLLDEQFVSALYRASQAGVKVEI
                     VVRGICALKPGIVGLSENITVRAILGRFLEHSRVYHFHNAGKDEYWIGSADLMNRNLD
                     RRIETLISIQSQSHKEQINQLLSLYLSPEVKRWQMDSEGLWKRVLLSEANEELIDIQE
                     HLIGESRARR"
     gene            4142..5035
                     /locus_tag="PLW53_05640"
     CDS             4142..5035
                     /locus_tag="PLW53_05640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD36936.1"
                     /translation="MLDDNSILAAGALIWRRNESDQIEIAFIHRIKYDDWSIPKGKLE
                     GDESLIACAYREVMEETGFSVRFGQSLGSISYQVGQRSKSVTYWAAKYLASPGSPNPS
                     EVDEVRWVLASDADSFLTRTLDKEIVKRFLLMDPDSKPLVLLRHAKAIERVEWAGEDT
                     DRPLSSIGERQAKRLISSLAPYGIEEIHSSSAVRCYESITPLARAQNIDFFFTDSLSE
                     DVYKKDKERAAKYVNKLLVNNYSALVCSHNPVLPAILSSFVDKFGIEVSEVKLEPGDA
                     WVVHHVEREVVGVDFLPVPKI"
     gene            complement(5032..5949)
                     /locus_tag="PLW53_05645"
     CDS             complement(5032..5949)
                     /locus_tag="PLW53_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRD36937.1"
                     /translation="MRLGVLDVGSNTVHLQVVDASPGARPNPNINHKEDLRLAEYLSA
                     DGFISAEGARLLREAIKRALVAAKSAEIDELLPFATSALREAVNGAEIIARINEDFEI
                     DLQVLSGEDEARITFLAARRWYGWSSGRLLMVDIGGGSLEIAVGVNEVPDVALSLPLG
                     ASRLTKDHLRGDPFTKKSIRNLRDHIERDIAEILPTIVQHQDSDRSIATSKTLRTLAR
                     ICGDWIDGNGKKLRIDSLRKITPRLAEMSQEDRAELPGVSNTRAKQITAGAFVAEAVM
                     RNLDIEELEICPWALREGIVLKWLDWMES"
     gene            6012..>6301
                     /locus_tag="PLW53_05650"
     CDS             6012..>6301
                     /locus_tag="PLW53_05650"
                     /inference="COORDINATES: protein motif:HMM:NF022674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HRD36938.1"
                     /translation="MSKAKKSKGADPFQAAELARSAALEDAKDQKYVGSLLSIEADDE
                     RIATYLFEANLPGYQGWNWAVTVAKVDSQSAPTICDVVLLPGAGALLAPDWIP"
BASE COUNT         1779 a         1310 c         1484 g         1728 t
ORIGIN      
        1 cctttcagat cttgaggtgc ggattctgga ttttgagcgc caatggtgga aatacgccgg
       61 ggcaaaagat gctgcaatta gagagctctt tgatctatca actagaagct attacgaact
      121 gctcaataat ttaattgacc gagaagatgc cctcgccgcc tctcctcttc tagtaaagcg
      181 cctaaggcgc cttcgccagg ctcgaatttc agctagatcc cgctaaggcc aaggcagggc
      241 cagcccggcc cctcaagaac ctgatttgag aacgggaata gagccctcta ggcttggcgt
      301 tagcactcgg caggtgagag tgataagtca cctgaagctc gaaaaaactg gttaattaaa
      361 tgaggagtaa atagaactat ggcaaagatg attgccttta acgaagatgc tcgtcgcggc
      421 cttgagcgcg gaatgaatac cttggctgat gcagtcaaag taacccttgg gccccgtggt
      481 cgaaatgttg ttttggaaaa gaagtgggga gctcccacaa tcaccaatga tggcgtctca
      541 attgctaaag agattgaact tgatgatcct tgggagaaga ttggcgcaga gttagttaaa
      601 gaagttgcta agaagactga tgatgtcgca ggagatggaa caactactgc aacagttctt
      661 gctcaagcac ttgtgcgcga aggtttaaga aatgtcgctg ccggctctaa tccaatggcg
      721 ctaaagaaag gtattgagaa ggctgttgaa gcaatttcaa ctgagctctc tgcaatgtca
      781 aagccagttg agacaaaaga gcagattgca gcaactgcat ctatctctgc tgctgactcc
      841 acaatcggtg agatgatcgc agaggcgatg gataaggttg gcaaagaggg agttatcaca
      901 gttgaggaga gcaacacctt tggtcttgag ctagagctca ccgagggaat gcgctttgat
      961 aaaggatata tctcgccata tatggttaca gatactgagc gcatggaagc ggttatggaa
     1021 gatgcttatg tgcttatctg taactcaaag atttcaaata ttaaagatct agtgccaatc
     1081 cttgagaagg taatgcaatc aggtaagcca cttgcaatta tcgctgaaga cattgaaggc
     1141 gaagcgcttg caaccttggt tgtaaataag atccgcggca ctttccgctc tgttgctgta
     1201 aaggcaccag gttttggtga tcgccgcaaa gcgatgctgc aagatattgc tatcttgact
     1261 ggcggaactg tgatctctga ggaagttggt ctaaagcttg aggctgcaac ccttgatcta
     1321 cttggcaaag ctcgcaaggt agtagttact aaggatgaga ccacaatcgt tgagggcgca
     1381 ggcgatgatg aaatgatcaa aggccgcgtc aatcagatcc gctctgagat tgaaaagact
     1441 gactctgatt atgatcgcga aaaacttcaa gagcgtcttg caaaacttgc aggaggagtt
     1501 gcagttatta aagctggagc ggctactgaa gttgagctaa aagagcgtaa gcaccgcatt
     1561 gaagatgctg ttagaaatgc taaagcagca gttgaagagg gcatcgtctc aggcggcgga
     1621 gtggcacttc tccaagcttc aaagaaagca tttgagaagc taaaacttac tggcgatgaa
     1681 gcaacgggag caaagattgt tgagcttgcc gttgaggcgc cacttaagca gatcgctatg
     1741 aatgctggtc ttgaaggcgg agtagttgtt gaaaaagttc gcaatcttgc agcaggccat
     1801 ggcctaaatg cagcaactgg tgaatacgtt gacatgatca aagctggaat tattgatcct
     1861 gccaaagtaa ctcgctcagc gctacaaaac gctgcctcga ttgcagctct ctttctaacc
     1921 actgaagctg ttattgctga taagccagaa cctaaatctg cggctcctgc agccccaggt
     1981 ggggatatgg acttctaatt aaataggttg ttaaccagat aaacgcccag tatttactgg
     2041 gcgtttactt tatccctaaa cgattgagcc ttaaccatgg aaaaactaga cgttctctcc
     2101 cctgaattca ttattgatcg tgagttgagt tggcttgatt ttaatactcg cgttcttgaa
     2161 cttgctgaag ataccagcat tccattactt gaaagagtgc gctttcttgc aattttctct
     2221 tctaatttag atgacttctt tatgattcga gtcgctgcga ctaaattaaa aattgagtct
     2281 ggggccagtg cgccaaatgc ctccggctta actccagttg ctctacttga agagataact
     2341 cagaaagttt cagagttgat taagcgtaag gttgagtgtc ttcacaactc aataaagcca
     2401 gggttagtaa aagctgacat tcacatggtt cactggaatg aactcactca agctgagcga
     2461 agctatgttg gagaaatatt tgatagtcag atatttccag tcttaacacc tcttgcggtt
     2521 gatccttcac acccttttcc ttatatctca gggctctcgc taagtcttgc ggtaattgtt
     2581 aagcatccag aaagcggcga ggagttcttt gcacgagtta aggtgcctag cgttatcgct
     2641 cgttttatct ctactcagaa atcaatcaaa acacgtttca tcgccctgga agagataatt
     2701 gcagctcacc taacagagct atttccagga atgataattg ttgatcacta tgcatttaga
     2761 ttgaccagaa atgaagattt agatctagag gaagaagata gtgaaaatat cctagagact
     2821 atggagcaag agctcttgcg tagaaagttt ggcccaccag ttcgtcttga agttgattct
     2881 gaaattcaag atgatctcct agatcgctta attagtgaac ttgagatcac tgagcgcgag
     2941 gtaattaaat atcaaggtcc actggattta acgggattaa atactctggc agatcttgat
     3001 aggccagatc tccactttcc cccctttaaa tcccagatgc caactggtct tgtagatatt
     3061 gaagatttag gaacagatgc gctctttaat ctcattaatc agggcgagat tctccttcat
     3121 catccctatg attccttctc atcttcagtg gttagattta ttgaggcagc tgcgattgat
     3181 ccaaaagtat tagctattaa acagacttta tatcgaacct caggtgattc accgatcatg
     3241 gaagcgctaa ttgaagcagc gcaggctggt aagcaagttc tcgccgttgt agaaattaga
     3301 gcgcgctttg atgagctagc aaatgttcgt tgggcaagaa agttagagga ggctggtgtc
     3361 catgtggtct atggccttat gggccttaag actcatgcca aactctcact tgttataaga
     3421 caggaagagt caggattaaa tagatactgc catgtaggaa ctggaaacta caatccaaag
     3481 acggctcgtc tctatgaaga tttaggtttg ctaagtgcag atccagttct ctgcgacgat
     3541 ctctcacgtc tatttaatca actctctggc tttgttaatg atatcgagtt taatcgcttg
     3601 attgtggctc caaaatcact tcgtaagtca ttaattaata aaatagatcg tgagatcgaa
     3661 aatgctagag aaggaaaacc agcgcatatt cggtttaagt taaattctct tcttgatgag
     3721 caatttgttt cagcactcta tagagcttct caagctgggg taaaagttga aattgtcgtt
     3781 agaggaatct gtgctttaaa gcctggcatt gttgggctct ctgagaatat aacggtgcgc
     3841 gccatattag gaaggttttt agagcactct cgggtttatc acttccataa tgccggtaag
     3901 gatgagtatt ggattggaag cgctgattta atgaatcgaa acctagatcg cagaatagaa
     3961 actttgatta gcatccaatc ccagagccat aaagagcaga ttaatcaact actaagcctt
     4021 tatctaagcc ctgaagtaaa gagatggcag atggatagcg agggtctttg gaagagagtt
     4081 ttactctcag aggccaatga agagctaatc gatattcaag agcatttgat tggagagtct
     4141 cgtgctagac gataattcta ttcttgctgc tggagcgctc atctggcgga gaaatgagag
     4201 cgatcaaatc gagattgctt ttattcatag aattaaatat gacgattggt caatccctaa
     4261 gggaaagcta gagggggatg aatccttaat cgcctgcgct tatcgagagg tgatggagga
     4321 gaccgggttc tcagttcgct ttggtcaatc tctaggaagc atctcttatc aagtagggca
     4381 gaggtcaaag tctgttacct attgggcggc aaagtatttg gctagcccag gctcacctaa
     4441 ccccagtgaa gttgatgaag ttcgatgggt actagcaagt gatgccgatt catttcttac
     4501 tcgcactctt gataaagaga tagttaaaag atttctcttg atggatccag atagcaaacc
     4561 actagttctt ttaagacatg caaaagctat cgagagagtt gaatgggcgg gagaggatac
     4621 cgatcgtcca cttagctcta tcggtgagcg gcaggcaaag cgtttgattt caagtcttgc
     4681 gccatatggc attgaagaga ttcattcctc tagcgcagtg cgctgctatg agagcataac
     4741 tccactagct cgagcgcaaa atatagattt tttctttaca gatagcctct ctgaggatgt
     4801 ctataaaaag gataaagagc gggcagcaaa atatgttaat aagttactgg taaataatta
     4861 cagcgcttta gtctgtagcc acaatccagt acttcctgcg atactttcaa gttttgttga
     4921 taagttcgga atagaagttt ctgaagttaa attagaacca ggtgatgctt gggtagtcca
     4981 ccatgttgag cgcgaagtag ttggtgttga ttttctccct gttcctaaga tttaagactc
     5041 catccaatca agccacttta aaacaattcc ctcccgtagg gcccaaggac agatctccaa
     5101 ttcttcaata tcaagatttc gcataacagc ctcagccaca aatgctcctg cagttatctg
     5161 ttttgcgcgg gtatttgaaa ctcctggaag ctcagctcga tcttcttgtg acatctctgc
     5221 aagccttgga gtaattttgc gtaaagaatc tattcttaac ttctttccat ttccatcaat
     5281 ccaatctccg cagattcgag ctagcgttct aagcgtttta ctagttgcga tagagcgatc
     5341 actatcttgg tgctgaacta tcgtgggaag tatttcggcg atatctcgct caatatgatc
     5401 tctaaggttt cgaattgatt tcttggtgaa gggatcgcct cgtaggtgat ctttagttaa
     5461 ccgtgaggcg ccaaggggta gcgagagcgc cacatcagga acttcattaa caccaacagc
     5521 aatttcaagt gatccgccac cgatatcaac cattaataac cgtccacttg accagccata
     5581 ccaacgcctt gctgccagaa aggtaatcct tgcttcatct tcgccgctta atacttgaag
     5641 atcaatttca aaatcttcat taattcgagc gattatttct gcaccattta ctgcttcacg
     5701 aagcgctgaa gttgcaaagg gaagcagttc atctatctca gcgctctttg cagcaacaag
     5761 ggctcgcttg attgcctccc ttagtaatcg cgctccttct gctgagataa aaccatcggc
     5821 tgataaatat tcagcaagtc ggagatcttc tttatggttg atgttgggat ttgggcgcgc
     5881 tccaggagat gcatcaacaa cttgaagatg cacagtattc gagccgacat ctaaaactcc
     5941 aaggcgcacc tgcgtacttt actaaccttt tgctttgctt ccgccttacc agtgccaata
     6001 gacttagtct catgtccaaa gcaaagaaat caaagggtgc agatcccttc caagcagcag
     6061 agctagcgag aagcgctgcg ctggaggatg ctaaagatca aaaatatgtg ggatcgctgc
     6121 tttccatcga agcagatgat gagcgaattg caacttatct ctttgaggca aatcttccgg
     6181 gatatcaagg atggaattgg gctgtaacag ttgcaaaggt agatagtcag agcgctccaa
     6241 caatctgcga tgttgttctg ctgccaggtg ctggagctct acttgcacct gattggattc
     6301 c
//
LOCUS       DAOROD010000035         6024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_976_length_6024_cov_39.319987, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000035 DAOROD010000000
VERSION     DAOROD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_6024_cov_39.319987"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /gene="nadC"
                     /locus_tag="PLW53_05655"
     CDS             <1..725
                     /gene="nadC"
                     /locus_tag="PLW53_05655"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRD36939.1"
                     /translation="SNARFVTRKSGVIAGCLVAAAVLEQCGIKEYELLVKDGQEVSAN
                     TELIRLKAETRAILKAERTALNFLSHLSGIATITNLWVKEVSSSKTAIRDTRKTTPGL
                     RELEKYAVRMGGGLNHRMNLSDQALIKDNHIAAAGSVSEAINRVKKAAPGALIEVEVD
                     TLEQLKEALQCSVEIVLLDNMSIEQTKAAVEIARGSNTKLESSGGLKLENAAAYAATG
                     VDYLAVGALTHSAPVLDIGLDF"
     gene            726..1499
                     /locus_tag="PLW53_05660"
     CDS             726..1499
                     /locus_tag="PLW53_05660"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRD36940.1"
                     /translation="MLLTIDIGNTNTVLGIFSKEKMIDTWRVKTDARDTADELWLQYR
                     SLITGYELSGLSICSTVPSVLREARSMINRHFQDLPTTIIEPGVKTGVPLLVDNPKEI
                     GADRIVNTLAAHTLYNQGACIVVDFGTSTNFDVVSPKGEFLGGALAPGIEISVEALAS
                     RAAQLRKVELVKPKSVIGKNTVEALQSGTIYGFAGQVDGLVARISDELDALYPDNEKI
                     SVIATGGLAPLVLGISETIDYHQPDLTLIGLRLVHERNG"
     gene            1513..3000
                     /gene="lysS"
                     /locus_tag="PLW53_05665"
     CDS             1513..3000
                     /gene="lysS"
                     /locus_tag="PLW53_05665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRD36941.1"
                     /translation="MSENDLPEQLRIRREKRESLLGRGIAPYPVSVNRTASLKEIRER
                     FKDLATDSPSGVIESVAGRIIFKRDTGKLCFATLREGDGTELQAMFSLDKVGEAVLES
                     WKSDIDLGDIVSVTGEVITSKRGELSILANSYQLASKALRPLPNEHNPLNEETRVRQR
                     YVDLIVRPEARANARIRPKVLKSVRDTFDKYDFIEVETPMLQVMHGGAAARPFKTFSN
                     AYDMDLYLRIAPELFLKRCVVGGIEKVFEINRNFRNEGADSSHSPEFAMLEMYWAYAD
                     WQNMKSLTQEIVQNAALAIFGSHKVEHHDGRISDLGGNWRELDFYTAISDAAGLTVTP
                     ATSREELVKIAEKAGLKIDPAWIHGKLAEEIFEHFYSGKLNTPTFVMGFPVDTSPLVR
                     EHRSVKGIAEKWDLYIDGFELATGYSELIDPVIQRDRLIEQAKLGAKGDLEAMQVDED
                     FLRAMEHGMPPMGGVGMGIDRLLMALTGLGIRETILFPLVKPEEN"
     gene            3001..3498
                     /locus_tag="PLW53_05670"
     CDS             3001..3498
                     /locus_tag="PLW53_05670"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRD36942.1"
                     /translation="MSVIVRPARTTDVKSVRRIIDQYALQRRLLSKDTVKLYEDVQEF
                     FVAESDGKVIGCGALHVLWEDLGEVRTVAVDEGFRRQKIGNQILEAITNRANSLGLKR
                     LFCLTFETEFFAQHGFKVIQGTPVEADVYQQLLRSYDEGIAEFLDLESVKPNTLGNTR
                     MLKIL"
     gene            3659..>6024
                     /locus_tag="PLW53_05675"
     CDS             3659..>6024
                     /locus_tag="PLW53_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRD36943.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKGLESLGISLEAVRSQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKEAVAAGPAEGTP
                     STSLILDQFGRNLTTAAREGKLDPVIGREKEIERVMQILSRRTKNNPVLIGEPGVGKT
                     AVVEGLAQAIVKGDIPETLKDKQLYSLDLGALVAGSRYRGDFEERLKKVLKEIRTRGD
                     IILFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAALE
                     RRFQPIQVAEPSLTHTIEILKGLRDRYESHHKVSINDDAIVAAATLADRYIADRFLPD
                     KAIDLLDEAGSRLRIRRMTAPPELRAFDDKIADVRKEKESAIDGQDFEKAASLRDKEK
                     QLMAEKSEREKSWKAGDLDVVAEVDEELIAEVLSTATGIPVFKLTEAETSRLLRMEDE
                     LHKRVIGQEQAIKALSQAIRRTRAGLKDPKRPGGSFIFAGPSGVGKTELSRTLAQFLF
                     GDEDALIALDMSEYSEKHTASRLFGAPPGYVGYDEGGQLTEKVRRRPFSVVLFDEIEK
                     AHPDIFNSLLQVLEEGRLTDAQGRQVDFKNTVIIMTTNLGTRDISKSLALGFTNVNDA
                     LGNYERMKNKVQDELKNHFRPEFLNRIDDVIVFHQLSEVEIVQIVDLMITNLETRLKA
                     KDMGIELTSKAKAVLAHRGYDPVLGARPLRRTIQREIEDVLSEKILFG"
BASE COUNT         1757 a         1232 c         1418 g         1617 t
ORIGIN      
        1 taagtaatgc ccgctttgtt acaagaaaga gtggagtaat tgctggttgc cttgttgctg
       61 cagctgttct tgagcaatgc gggataaaag agtatgagtt attagttaaa gatggtcagg
      121 aagttagcgc taatactgag ttaatccgcc taaaggcaga aaccagagca atacttaaag
      181 cagagcgaac tgctctaaac tttctctctc atctctccgg tattgcaaca attactaacc
      241 tctgggtcaa agaagtttca tcgagtaaaa ctgctattcg cgacactaga aaaaccactc
      301 cagggcttag ggagcttgag aaatatgcag ttcgtatggg cggcggctta aatcatcgaa
      361 tgaaccttag cgatcaagct ttaatcaaag ataaccatat tgctgcagct ggctcagtta
      421 gtgaggcgat taatcgggtt aaaaaagctg cgccaggtgc tcttattgaa gttgaggttg
      481 atactttaga acagttaaaa gaggctctgc aatgcagcgt tgaaatagtg ctgctagaca
      541 atatgagtat tgagcagact aaagcagctg ttgaaattgc tagaggatca aacaccaaac
      601 ttgaatcatc aggtggccta aaactggaaa atgctgctgc ctatgccgca acaggtgttg
      661 attacttagc agttggtgcg ttaacccact cagccccagt actagatatc ggattggatt
      721 tctaaatgct attaactatt gatatcggca ataccaatac cgttcttgga atcttttcca
      781 aagaaaagat gattgatact tggcgagtta agactgatgc tagagatact gccgatgaat
      841 tatggttgca atatcgctct ctaattactg gctatgagtt aagcggcctt tcaatctgct
      901 ccacagttcc ctcagtactt agggaagcaa gatcgatgat taatcgccac tttcaagatc
      961 taccaacaac cattattgag ccaggagtaa agactggagt accgcttcta gttgataatc
     1021 caaaggaaat tggagctgat cgcatcgtaa atactcttgc tgctcatacg ctttataacc
     1081 agggcgcatg tatcgtcgtt gattttggca cctctactaa ctttgatgtc gtctcaccaa
     1141 agggtgagtt cttaggtggg gcgttagctc caggaattga gatctctgtt gaggctcttg
     1201 catcaagggc tgcgcagtta agaaaagttg aattagttaa acccaagtca gtgattggca
     1261 aaaacactgt agaggcgctt caatccggaa ctatctatgg ttttgcaggc caagtagatg
     1321 gccttgttgc tcgcattagt gatgagctag atgcgctcta tccagataac gagaagatct
     1381 cagtgatagc aactggtggt ttagcgccat tggtgctggg aatttcagag acaattgatt
     1441 atcaccaacc cgatttaacc ctgattggcc tacgtcttgt gcacgagagg aatggttaga
     1501 cttatcggca ctatgtccga aaatgacctt cctgagcagt taagaattag acgcgaaaaa
     1561 cgcgaatcat tgcttggccg tggcatagcc ccatatcccg tatcagttaa tagaacagcc
     1621 tcattaaaag aaatccggga gagatttaaa gatcttgcaa ctgatagccc atctggagta
     1681 atcgagagcg tagctgggcg aataattttc aagcgcgata ccggaaagtt atgttttgca
     1741 acacttcgtg aaggtgatgg cacagagcta caagcaatgt tttcgctaga taaagttggc
     1801 gaagctgttc tagagagttg gaagagtgat attgatttag gcgatatcgt gagtgttact
     1861 ggcgaggtaa ttacttcaaa gcgtggcgag ttatcaattc ttgccaattc ttatcaatta
     1921 gcctccaaag ccctacgccc acttccaaat gagcataacc cgctcaatga agagactcgg
     1981 gttcgccaaa gatatgtaga tttaattgtc agacctgaag caagagcgaa tgcaagaatt
     2041 aggccaaaag ttcttaagtc agtaagagat acctttgata agtatgactt tattgaagtt
     2101 gagactccaa tgcttcaagt catgcatggg ggagctgcag caaggccctt taagaccttt
     2161 agcaatgcct atgacatgga tctttatcta agaattgctc ctgagttatt cctgaaaaga
     2221 tgcgtggtag gtggcattga aaaagtcttt gaaataaata gaaatttcag aaatgaggga
     2281 gctgattcaa gccactctcc agagtttgca atgcttgaga tgtattgggc ctatgcagat
     2341 tggcagaata tgaaatctct aacccaagaa atagtccaga acgcagctct tgccatcttt
     2401 ggttcccata aagttgaaca tcatgatgga cgaattagtg atctaggtgg gaattggaga
     2461 gagctagatt tttataccgc catctcagat gccgcaggct taactgttac tccagctacg
     2521 agccgagaag agctagtaaa gattgctgaa aaagctggtc taaaaattga tccagcatgg
     2581 attcatggca agttagctga ggagatcttt gaacacttct atagcggcaa gttaaatacc
     2641 ccaacctttg ttatgggatt tccagttgat acctcaccgc tggttcgcga acaccgcagc
     2701 gttaaaggaa ttgctgagaa gtgggatctt tatatcgatg gctttgaatt agccactgga
     2761 tatagcgaat taattgatcc ggttatccaa agagatcgcc taatagaaca agcaaaactc
     2821 ggagcaaagg gtgatctaga ggcaatgcaa gttgatgaag atttccttcg cgctatggaa
     2881 catggcatgc ctcctatggg aggagttgga atgggaattg atcgactact gatggcctta
     2941 actggccttg gtattcgaga gacaattctc tttccattag ttaagccgga agaaaattaa
     3001 atgagcgtga ttgtcaggcc ggcaagaaca acagatgtta aatctgttcg caggattatt
     3061 gatcaatacg ctctgcagcg ccgcctgctt tcaaaagata cggtaaagct ctatgaagat
     3121 gttcaagagt tttttgttgc agaaagtgat ggaaaagtaa ttggctgcgg agcgcttcac
     3181 gttctctggg aggatttagg agaggttaga actgttgcag ttgatgaagg atttagaaga
     3241 caaaagattg gcaatcagat tcttgaagcc attactaatc gtgctaattc actaggcttg
     3301 aagagattat tttgtttgac atttgaaaca gagttttttg cgcaacatgg atttaaagtt
     3361 attcaaggaa ctccggtgga ggcagatgtc tatcagcaac tgctgcgctc ctatgatgaa
     3421 ggtattgctg agtttctaga ccttgagagt gtgaagccca acaccctggg taacaccaga
     3481 atgctcaaga ttctttaatc ttttcgggca taaaaaccta aatatggcgg ttatagcccc
     3541 tgtggattcc catatctagc gctgatttgg gggcaggaaa atagatgtcg catcgtttca
     3601 ggccttctta tacgctagag ccagtccgtt ttagcagttt cgataaggag atgaactgat
     3661 gtttgagcgc tttaccgatc gagcccgccg cgttgttgtt ctggcgcaag aagaagctcg
     3721 catgctcaat cacaattaca tcggaaccga acatatcctc cttggcctta tccatgaagg
     3781 tgaaggcgtt gctgcaaaag gtttagagtc tctaggaatt tcacttgaag cagttcgttc
     3841 tcaagttgaa gaaattattg gacagggaca gcaagctcca tcaggtcata ttccatttac
     3901 tccaagagct aagaaagttc ttgagctatc acttcgtgaa gctcttcaac ttggacataa
     3961 ttacattgga accgaacata ttcttcttgg ccttatccgc gagggagaag gcgttgctgc
     4021 gcaagttcta gtaaaacttg gcgcagatct caatcgagtt cgtcaacaag taattcaatt
     4081 actctccggt tatcaaggaa aagaagctgt tgcggcagga ccagcagagg gaactccatc
     4141 cacatcttta atccttgatc aatttggtcg taacctaact acagctgcgc gcgaaggaaa
     4201 actagatcca gtaattggcc gtgaaaaaga gattgaacgc gtaatgcaga ttctctcccg
     4261 tcgtacaaaa aataatccag tactaattgg cgagccaggc gttggaaaga ctgctgttgt
     4321 tgaaggttta gctcaagcca ttgttaaagg cgatattcca gagacgctaa aggacaagca
     4381 gctctactct ctagatctag gcgccctagt tgctggatct cgctatcgcg gtgattttga
     4441 agagcgctta aagaaagtat taaaagagat tcgcaccaga ggcgacatta ttttgtttat
     4501 cgatgaaatt cacacacttg ttggcgccgg agccgcagag ggcgcaattg atgcagctag
     4561 catcctaaag ccaatgcttg cccgcggcga gctccagaca attggcgcaa caactcttga
     4621 tgagtatcgc aaacacttgg aaaaagatgc tgcgctagag cgtcgcttcc agcctattca
     4681 agtagctgag ccatcactta ctcacaccat tgaaattcta aaaggccttc gtgatcgcta
     4741 tgaatcacac cataaagttt caataaatga cgacgcaatt gtcgctgctg caacacttgc
     4801 agatcgatac atcgcagatc gtttcttgcc agataaagcc attgatcttc tcgatgaggc
     4861 tggatctcgc ctacgtattc gtcgcatgac cgcgcctcct gagctacgcg cctttgatga
     4921 caagattgct gatgttagaa aagagaaaga atcagcaatt gatggccaag atttcgaaaa
     4981 ggctgcatca ctgcgcgata aagaaaagca attaatggct gagaaatctg agcgcgaaaa
     5041 atcttggaaa gccggagatc tcgatgtagt tgcagaagtt gatgaagagt taattgctga
     5101 agtgctctca actgcaacag gaattccagt atttaagcta actgaagctg aaacatctcg
     5161 cttgcttcgt atggaagatg aactccataa gcgtgtaatc ggccaagagc aagcaattaa
     5221 agctctctct caagctattc gccgcactcg cgcagggctc aaagatccta aacgcccagg
     5281 tggctcattt atttttgcag gtccatccgg agttggtaag acagaacttt ccagaactct
     5341 tgctcagttc ctctttggtg atgaggatgc actaatcgct cttgatatgt cagagtattc
     5401 cgagaagcac accgcatctc gcctctttgg cgctcctcca ggatatgttg gatatgacga
     5461 gggtggtcaa ttaactgaga aggttcgtcg ccgtccattt tctgtggtgc tatttgatga
     5521 gattgaaaaa gctcacccag atatcttcaa ctcactgctt caagtattag aagagggtcg
     5581 actaactgat gcccaaggac gtcaagttga tttcaagaac actgtaatca tcatgacaac
     5641 taaccttgga actcgcgata tctctaagtc cctagctctt ggatttacta acgttaatga
     5701 tgctctagga aattatgagc gcatgaagaa taaggtccaa gatgagctta agaatcactt
     5761 ccgcccagag tttcttaacc gcatcgatga tgtaatcgtc ttccaccagt tatcggaagt
     5821 tgaaattgtt caaatagttg atttgatgat tacaaatctt gagacgcgcc ttaaggcaaa
     5881 agatatggga attgaattga cttcaaaggc aaaggcagtg ctcgcccacc gcgggtatga
     5941 cccagtgcta ggtgctcgtc cacttcgtag aacaattcaa cgagagatcg aagatgtctt
     6001 gtcagagaag attctctttg gtga
//
LOCUS       DAOROD010000036         5588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1140_length_5588_cov_20.963492, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000036 DAOROD010000000
VERSION     DAOROD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_5588_cov_20.963492"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..777)
                     /locus_tag="PLW53_05680"
     CDS             complement(73..777)
                     /locus_tag="PLW53_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD36944.1"
                     /translation="MAKEEFLKPVRISELIRADLSELAIHEVIPTERQMAEHFGVSRV
                     TIQKALKILERSGFIYRRQGSGTFVAPRRDTHHHSLRSFSEELTLRGIEYKTSVLDVR
                     LIESPESIDSTWAKVTEPSYRIERVRTNRKVALSWEVSYVKARFAPGLDKEILSGSLY
                     KLLSENYGQVVESADEEISSLLIEDEISTKLSVLAGTPAIEIQRKAFNRRGDCLETAR
                     AIRPSGREFLKYSVRR"
     gene            948..2195
                     /locus_tag="PLW53_05685"
     CDS             948..2195
                     /locus_tag="PLW53_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD36945.1"
                     /translation="MRITRKKVIAIISSMALVSSLFLGASTASAAPKDSGTVTVWIQG
                     DAKSWPAAVDRANKMFNSKYPNVKVDIQYQTWGDSLKKLDAALVAGNTPDVFEFGNTQ
                     VLKYSAAGALKDLSSSKSQFAYSTNWLKGLEEAGSYNGKLYAVPYYAGSRAVMYRTDL
                     FASLKIKAPRTYEQFTAAADKLMAANSSNSKFSAVYMPGKYWYAAMGFVYDSKGAIAV
                     QEAGKWKGSLDSAASIEGLTRWKNIVDKYSKADKSGDEGGQWEVFAGGNVAMSYSNGW
                     ETCCIAPQKGAFFPMPSIRAGEYMPAFLGGSNLGVPAKAKNPEWGVYWIKSFTSGQAM
                     REIVKVGNLPNNTSMLTLVKGGNAQVAKGASSTWFVPAAEKWVNVENANVLQTMLSDI
                     ATGKKTVAQAAKDASAEITKLLN"
     gene            2289..3206
                     /locus_tag="PLW53_05690"
     CDS             2289..3206
                     /locus_tag="PLW53_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD36946.1"
                     /translation="MSSAAKLRLGPKKNLWPYLLITPALAVILLILGFPVFRLITLSF
                     QQFGLAEIVSGIPTWVGFENFQALLKDSEFWIVLRRTFIFTFAMVAASIVIGAWLAHL
                     MLRMNPILRWLLNTTLILVWAIPALVGISIWKWMFSFEFSIITSTINRLGFDFAQRNW
                     FTNTFSGFSIIGGVVVWGAIPFITISIYAALTQVPKELLEAAAIDGANSRQIFRSIIL
                     PILLPIYVILISLSIIWDFQVFTQIWVLLDNRPASEYYTMAIYAFEKSFGLSEYGMGA
                     AIALLMIFSLLGVTWFYIKKMVQIGDGNA"
     gene            3193..4023
                     /locus_tag="PLW53_05695"
     CDS             3193..4023
                     /locus_tag="PLW53_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRD36947.1"
                     /translation="MAMHRKSSIYANISGFFAIIFMGFPVYWMVTTSFKNRQDVLSTE
                     PTILPRSFTFENYQNAFSREGFYTSLRNSLIVVLITVAISLFLATFASVAIARTKFTG
                     KRSFISALLIVQMLPLSALIIPLFLLLTRLQLTNTIGGLALTYVAFILPFTIWTLRGF
                     LANIPTDIEEAALVDGCTRVQAYRHVLLPLMAPGLVATAIFAFIQAWNEFLLAYIIMQ
                     SQENLTLPVWLAGFTTRTGTDWGPLMAASTVTAIPVVIFFSLIQKRIATGVTAGAVKG
                     "
     gene            4020..5465
                     /locus_tag="PLW53_05700"
     CDS             4020..5465
                     /locus_tag="PLW53_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRD36948.1"
                     /translation="MKFNNTEKAILAHFSPGFGGTEIPDWIFKYLENGLGGITLFSSN
                     CPSLDVTRELILKIRAISPNIIISLDEEGGDVTRLFVPEGSPFPTPALLGRCNDLELT
                     ESSFKELGKILKSIGVDLNLAPVADVATQSDNPIVAVRSFGNDAELVSKHVVSAVKGL
                     RSAGIASCIKHFPGHGGVLEDSHHDLPQLNGEMDELINTHLKPFISAIGDGVEAIMMG
                     HILLAAIDSTSPASCSSRITRSLLRKSLGFEGLVVTDALDMGALGGTKKIHTSALRAI
                     SAGADLLCFSGLYDQSSFVDNGLVAIREAIEKGEIERQAIEDNAEKIWRWRAPVATEL
                     SPVTLPEASKFKSGVNYQGNLALSTDQISLIELSADPTIAAGFVGWGLRRPFIRAGIK
                     VKLESSDIDLSTHNQVCLVVAFRDAFRDNKILVALDRINRSRPDAIFVDMGWPTWSFN
                     PKNIIRTFGSSALASEIAVAILTESPISSKR"
BASE COUNT         1508 a         1179 c         1239 g         1662 t
ORIGIN      
        1 cccaggcctt gcggttgcaa tccgtgatcg cattaataga gcagcagcga attagattgt
       61 gacagaggct ttttagcgtc taacgctgta tttcaaaaac tctctaccgc ttggtcgtat
      121 tgcccttgct gtctcaaggc aatcgccgcg tctgttaaaa gcttttcttt gaatttcgat
      181 tgctggagtg ccagccaata ctgataactt ggttgagatt tcatcttcaa taagtagtga
      241 tgaaatttct tcgtcagcgc tctctacaac ctgcccataa ttttctgaaa ggagtttgta
      301 taaagaccct gaaagaattt ctttatcaag cccaggagca aatcttgctt ttacatatga
      361 aacttcccaa gagagagcta cttttctgtt tgttcgaact ctctcaatac gataagacgg
      421 ctcggtaact tttgcccaag ttgaatctat cgattcagga ctctcgatta acctgacatc
      481 caagactgaa gttttatact cgattcctct cagcgtgagt tcttcactaa aggacctaag
      541 gctgtggtga tgtgtatcgc gacgaggagc gacaaatgtt ccagagcctt gtctcctata
      601 aataaatcca gagcgttcta aaatctttag cgctttttgg atagtcactc gtgagactcc
      661 aaagtgctca gccatctgtc tttcggttgg aataacttca tgaatcgcta gttctgagag
      721 atctgcgcga atcaattcag agatgcgaac aggttttagg aactcctcct tggccatggc
      781 ccccgccccc cttcttcgcc tcacttttta agggaggaga taccgattcc ataacaatcg
      841 aaagaaaaac ctgtcggata ctagcatttt gaccaaaatt ggactagacc aacctactaa
      901 gaacctgttg gaattgccaa cgggtagctc taaacgggag gtccctcatg cgcattacgc
      961 gcaaaaaagt tatcgccatt atttcttcga tggcgcttgt cagctcgcta tttcttggcg
     1021 cgagcacagc aagtgctgcg ccgaaagata gcggcacagt aactgtctgg attcaaggag
     1081 atgcaaagtc ctggccagca gcagttgatc gtgcaaataa gatgtttaat tcaaaatatc
     1141 caaatgtcaa ggttgatatc caatatcaga cctggggcga ttcacttaag aaacttgatg
     1201 cagccctagt agctggaaat acgccagatg tatttgaatt tggtaatact caagttctta
     1261 aatattcagc tgcgggtgct cttaaggatc taagcagttc gaagagccaa tttgcatata
     1321 gcaccaactg gcttaaaggc ctagaagaag ccggaagtta taacggcaag ctttatgcag
     1381 ttccttacta tgctggatca cgtgcagtta tgtatcgcac tgatttattt gcttcattga
     1441 aaattaaagc accaagaact tatgagcaat ttactgcagc tgcagataag ttaatggccg
     1501 caaattcttc taactctaag ttctcggctg tctatatgcc aggtaagtac tggtatgcag
     1561 caatgggctt tgtttatgac tcaaagggag ctattgcagt ccaggaagct ggaaagtgga
     1621 aaggctcact agattcagca gcatccattg aaggattaac tcgctggaaa aatatcgttg
     1681 ataaatactc caaagccgat aagtccggcg atgagggtgg tcaatgggag gtcttcgcag
     1741 gcggaaacgt tgctatgagc tactcaaatg gttgggaaac atgctgtatt gctcctcaaa
     1801 agggcgcttt cttcccaatg ccttcaattc gcgctggtga atacatgcca gctttcctag
     1861 gtggatcaaa tctcggtgtt ccagcaaagg caaaaaatcc tgaatggggc gtctattgga
     1921 tcaagtcatt cacctcaggc caagctatgc gagagattgt caaagttggc aatcttccta
     1981 acaacaccag catgttgact ctagtaaagg gtggaaatgc tcaagttgct aagggtgcta
     2041 gctcaacctg gttcgttcct gcagcagaga aatgggtaaa tgttgagaac gcaaacgttc
     2101 tgcaaaccat gctctctgat atcgcaacag gaaagaagac agttgcgcaa gcagctaaag
     2161 atgcatctgc ggagattaca aaactgctga actagcggta aaaaactcct aacgaggtat
     2221 aaggagagtg gtagcgtcga gggcgctcca ctctccactc atttattggg aagtaggtat
     2281 tagatttcat gtctagcgca gcaaaattga ggctaggacc aaagaagaat ttatggccct
     2341 atctcttaat tactcctgcc ctggccgtga ttcttcttat cttaggcttc ccagtatttc
     2401 gactcataac tctcagtttt cagcaatttg gccttgcgga aattgtttct ggaattccaa
     2461 cttgggttgg ctttgaaaac tttcaagcac tgctaaaaga ttcagagttc tggatagtct
     2521 tgcgcagaac atttattttc acctttgcca tggttgctgc ttcaatagta attggtgctt
     2581 ggttagcgca tttgatgctt cgaatgaacc ccatacttag atggttactt aatacaactc
     2641 ttattctggt ctgggcgata cctgctctag tcggtatttc gatttggaag tggatgtttt
     2701 cctttgaatt tagcatcatt acctcaacta ttaatcgtct tggttttgac tttgcccaac
     2761 gtaattggtt taccaatacc ttttcagggt tttcaattat tggcggagta gtggtttggg
     2821 gcgctatccc atttataaca atcagtattt atgcggcttt aactcaagtt ccaaaagagc
     2881 tactggaggc agcagcaatt gatggcgcga actcgagaca aatatttaga tccataattc
     2941 tcccaatact tcttccaatt tatgtgattc taataagcct ttccataatc tgggattttc
     3001 aagtctttac ccaaatctgg gtgcttcttg ataaccgacc ggcttctgag tattacacga
     3061 tggcaattta tgcttttgaa aagtcttttg gtttgagtga atatgggatg ggcgctgcga
     3121 ttgccctttt gatgattttc tcattattag gtgtcacctg gttctacata aagaaaatgg
     3181 ttcagattgg agatggcaat gcatagaaag agttcgattt atgcaaatat ctctggattc
     3241 tttgccataa tctttatggg gtttccagtt tattggatgg tcactacatc atttaagaat
     3301 cgtcaggatg tactttcgac tgaacccaca attttgccga ggtcttttac tttcgagaat
     3361 tatcagaatg ctttttcaag agagggcttc tacaccagtt taagaaatag tttgattgtc
     3421 gtgcttatca ccgttgccat ctcgttgttt ttagcaactt tcgcaagtgt tgcaatcgca
     3481 cgcactaaat ttaccggtaa gcgaagcttt atctcagcgt tgcttattgt gcagatgctc
     3541 ccactctctg ccctgataat tccgctattc ctgctattga ctcgactcca actaacaaac
     3601 accatagggg gattagctct cacatatgtg gcatttattt tgcctttcac tatttggacc
     3661 ctgagaggtt ttctagccaa tattccaaca gacatcgaag aggctgccct agttgatggt
     3721 tgcacgagag ttcaggcata tcgccatgtc ctcttgccat taatggctcc aggattggtt
     3781 gccaccgcaa tatttgcatt tatacaagct tggaatgagt ttttgttggc ctacataatt
     3841 atgcagagtc aagagaacct aaccctgcca gtttggcttg ctggttttac gactagaacc
     3901 ggcaccgatt ggggcccatt aatggcagca tccacagtca cagctattcc agttgtaatt
     3961 ttcttctcac tgattcagaa gcgaattgct acaggcgtta cggcaggagc tgttaaagga
     4021 tgaaattcaa taataccgag aaagcaatat tggcccactt ttctcctgga ttcggcggca
     4081 cggagatccc ggattggatt tttaaatatc ttgaaaatgg gcttggagga ataacgctat
     4141 ttagttcaaa ctgtccttct ttagatgtaa cacgtgagtt aatccttaag atcagggcta
     4201 tctcgccaaa tattattatt tccctagacg aggaaggtgg tgatgtaact cgtctatttg
     4261 ttcctgaagg aagcccattc ccaacccctg cacttcttgg acgttgcaac gatttagaat
     4321 taacagaaag ctcctttaag gagttaggta aaattctaaa gtctattggt gttgatctaa
     4381 atctagctcc agttgctgat gttgcaactc aaagtgataa tccaattgtt gcagttcgct
     4441 cttttggtaa cgatgctgaa ttagtttcaa agcatgtagt cagtgcagtc aagggtttgc
     4501 gaagcgctgg aatcgcatca tgtattaagc actttcctgg ccacggaggg gtattggagg
     4561 atagccatca cgatttacct cagctaaatg gtgagatgga tgaactgatc aatacccatc
     4621 tcaagccatt catctcagca attggagatg gagtagaggc aataatgatg gggcatatct
     4681 tgcttgctgc aattgattcc acctccccgg cttcgtgctc aagtcggatt accaggagtt
     4741 tgctccgtaa aagtcttggc tttgaaggtt tagtggttac cgatgcacta gatatgggcg
     4801 cacttggagg aacaaagaaa attcatacct cagctcttcg agcgatatcg gcaggcgcag
     4861 atctactctg cttctctggt ctctatgatc aatcaagttt tgttgataat ggtttggttg
     4921 caattcgaga ggctatagag aagggagaga ttgaaaggca ggcaatagag gataacgcag
     4981 agaaaatctg gcgctggaga gcgccagttg caaccgaact gtctccggta actttgccag
     5041 aggcttcaaa gtttaagtct ggagttaatt atcaaggtaa tctcgcttta tcaaccgatc
     5101 agatctctct aattgagcta tctgctgacc caacaattgc agctggcttt gtcggttggg
     5161 gattacgaag accatttata agagctggca ttaaggttaa attagaatca agcgacatcg
     5221 atctttcaac tcacaatcaa gtttgtctgg ttgtggcctt tcgggatgct ttccgagata
     5281 acaaaatact agtggctcta gatcgaatca atcgaagtag gccagatgca atatttgttg
     5341 atatgggatg gccgacttgg agttttaatc ctaaaaacat tattagaact tttggatcaa
     5401 gtgcacttgc ctcagaaatc gcagtagcta ttttgacaga gagtcctatt agctcaaaga
     5461 gataagttca aagtaattct ctacgattcc accaattctg actttaggag aaactgtcgt
     5521 cacttcgccc agcgaccaat taggtggatt ttccttgcca cttaaagccc aagctgcctg
     5581 tttcgctg
//
LOCUS       DAOROD010000037         5365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1240_length_5365_cov_28.995669, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000037 DAOROD010000000
VERSION     DAOROD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_5365_cov_28.995669"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /gene="xylB"
                     /locus_tag="PLW53_05705"
     CDS             complement(<1..1298)
                     /gene="xylB"
                     /locus_tag="PLW53_05705"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRD36949.1"
                     /translation="MPIVAGVDSSTQSVKVVLRDSDSGELIASTSRPHPDGTEIDPQY
                     WWKALSDALDELGSHKISAISIAGQQHGMIALDKDGAVIRDALLWNDTRSDKAALDLN
                     QEIPDIALRTGSQLVASFTASKLRWLADNEQENANKVAAIALPHDWLSWKLSGAKSLD
                     TLFTDRSDASGTGYFDSVKNEYCLDILATAIRNSRKVTLPKVVVPNQFGATSIDSIPI
                     AAGAGDNAGAALGLGASLGDLVISLGTSGTAFAVSKSSTHDSSGEVAGFADATGNFLP
                     LACTLNAAKIFNTVARSLSLSFEEFSSLALSAKAGAEGLRMIPHFDGERTPNKPRAKG
                     SFHGITHSNFTKENIARASIEGVIAGMIYAARAVERLGVSYSRILLIGGAAKNSAVQQ
                     ISADLFGREIYIPAIGEYVADGAAKQAAWALSGKENPPNWS"
     gene            complement(1304..2455)
                     /gene="xylA"
                     /locus_tag="PLW53_05710"
     CDS             complement(1304..2455)
                     /gene="xylA"
                     /locus_tag="PLW53_05710"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRD36950.1"
                     /translation="MRNPTPADKFTFGLWTVGWQARDPFGDATRAALDPIRTVSELAK
                     RGAYGVTFHDDDLIPFGSSTSDRAAHIARFKKALDENGMKVPMATTNLFSHPVFKDGA
                     FTSNNRDIRRYAIRKVMTNIELAVELGAHTYVCWGGREGAETDIGKDPVVALERMREA
                     FNILGQFVIDKGYNIKFAMEPKPNEPRGDIFLPTIGHALAFINSLDHPELVGLNPEVG
                     HEQMAGLNFVHGIGQALFHKKLFHIDLNGQHGPKYDQDLVFGHGDLKSAFFLVDLLER
                     YNYDGPKHFDYKPMRTESDDGVWASATANMRTYIILQERAKAFRADPRTAAALEKSGV
                     NELLQPTVAKGEGWKEIAGESFDVEAAGKRGYHYEELDQLALEYLIGVY"
     gene            2498..2839
                     /locus_tag="PLW53_05715"
     CDS             2498..2839
                     /locus_tag="PLW53_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HRD36951.1"
                     /translation="MLEKSDRPWGRYEVLQESPSHKVKCIWVSPGKRLSYQRHQKRSE
                     HWFIVSGSAQVIINGVVSSPSAGDSFDIPAGALHRIANVGDNDVVFIEVQTGTYFGED
                     DIERLEDDFGR"
     gene            2847..3884
                     /locus_tag="PLW53_05720"
     CDS             2847..3884
                     /locus_tag="PLW53_05720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRD36952.1"
                     /translation="MNVKDFKMFSEISETPEVFSRLINSKAQFQLAAEKIKARDITNV
                     IILARGTSDNAGHYLKFLIEVKLGLPVGLASPSSVSIYGAKVDFKNTLVIALSQSGRS
                     PDLITFASAAREGGALLIAMTNNSDSPLAKAGDIHIDLSAGPEVAVAATKSYSAELLA
                     SLLLVDSWMGNKEEARAHLVDSSRECIANLSQVDSFANSLDAAREIVVIGRGYSYANA
                     KELALKIQETSYIPVQGMSSADYQHGPIATLNKNSQVIVLSPSGMPKKALEDSIARIR
                     QSGPELIWLGSNQLALREEKVIKGSTQASEEESTIIDAALIQYLTLRFAIKNGFNPDS
                     PAGLSKVTETL"
     gene            complement(3868..5196)
                     /locus_tag="PLW53_05725"
     CDS             complement(3868..5196)
                     /locus_tag="PLW53_05725"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HRD36953.1"
                     /translation="MILKKWGEMFTWGVSTSSYQIEGAANQGGRGPSIWDTFSKIPGA
                     VANGDNGDVACDHYHRFNEDLDLMKWLGVGAYHFSIAWPRVIPSGYGALNKEGMDFYD
                     RLIDGALERGITPWPTLYHWDLPQSLQDKGGWNNRDCAYWFAEYSQKMAEAFSDRLKN
                     WITINEPFCSAWLGHLYGVMAPGIKDLQTGINASHHLLLGHGLATKAIREVSSELKVG
                     ITLNFTPAITLGQRSDDKLAVELADGFDNRWFGDPVFKAKYPEDIVKAFGKEVPINPG
                     DMEIISTPLDYLGLNYYFRQTVAYDPSAKPLPYKQVTAPNVERTGMGWEVHAQSFTEL
                     LERINREYQPKEIFITENGSAWDDEVIDGKVDDPKRVSYLERHLDAMFAAKDNGVPIS
                     GYFAWSLIDNFEWAYGYAKRFGIIYVDYQTQKRIPKSSAYYYRERIKGSQ"
BASE COUNT         1501 a         1214 c         1127 g         1523 t
ORIGIN      
        1 gaccaattag gtggattttc cttgccactt aaagcccaag ctgcctgttt cgctgcgcca
       61 tctgccacat actcaccaat tgctggaata taaatctctc ttccaaacaa atcagcagat
      121 atctgctgca ctgcagaatt tttggcagct ccaccaatta gtaaaattct ggaataacta
      181 acgcccaacc tttcaacggc tcttgcagca taaatcattc cagcgataac tccttcaata
      241 gatgcacgcg caatgttttc ttttgtgaaa ttgctatgag ttatgccatg gaaggagcct
      301 ttggcgcgtg gtttgttagg agttctctcg ccatcaaagt gaggaatcat tcttaaaccc
      361 tctgctccag cttttgcaga gagggcaagg gagctaaatt cttcaaacga aagcgagaga
      421 cttcttgcaa cagtattaaa tatttttgca gcatttaagg tgcaagcaag gggaagaaaa
      481 ttgccggtcg catctgcaaa ccctgcaact tcgccagatg aatcatgagt tgaactcttt
      541 gagacagcaa aagcagttcc acttgttcca agtgagataa caagatcgcc cagagatgcg
      601 ccaagaccaa gcgccgcacc tgcattatct ccagccccag cagctattgg gatggaatca
      661 atgctagttg cgccaaactg atttggaaca actacctttg gcaaagtcac tttcctacta
      721 tttctaattg ctgttgccag aatatctaag caatactcgt tttttactga atcaaaatat
      781 ccagttccag atgcatcaga gcgatcagta aacagagtat ctagagattt tgccccagag
      841 agtttccaag agagccaatc atgaggaaga gcgatagcag caaccttgtt agcattctcc
      901 tgctcattat cagcaagcca tctaagcttt gatgcagtaa atgaagctac taattgagag
      961 ccagtgcgaa gcgcaatatc tggaatctcc tggttgagat caagcgctgc tttatccgaa
     1021 cgagtgtcat tccagagcag ggcatctcga attactgctc catctttatc aagtgcgatc
     1081 atgccgtgtt gttggccggc aattgatatc gctgaaatct tatgtgaacc taattcatca
     1141 agagcatctg atagagcctt ccaccaatac tgcgggtcaa tttctgtccc atctggatgc
     1201 ggccttgaag ttgatgcaat caactcacca ctatcagaat ctcgcagaac aacttttact
     1261 gattgtgttg aggaatcaac acctgcaact atcggcatgg tttctagtaa acaccgatta
     1321 gatattccag agctagctga tcgagttctt catagtgata gccgcgcttt cctgccgctt
     1381 ccacatcaaa actctcgccg gcaatctctt tccagccctc accctttgca acagttggct
     1441 gaagcagctc attaacacct gatttctcaa gagctgcagc agttcttgga tctgctctaa
     1501 atgcttttgc tcgctcctgc aagattatgt aggtgcgcat atttgcagta gctgaggccc
     1561 agacgccatc atcgctctcg gttctcattg gtttgtaatc aaagtgctta gggccgtcat
     1621 agttatagcg ctcaagaaga tctaccaaga agaaggcaga ttttagatcg ccatggccaa
     1681 agactaaatc ttggtcatat ttagggccat gttggccatt tagatcaata tggaagagtt
     1741 ttttatggaa taaagcttgt ccaattccat gaacaaaatt aagtcctgcc atctgctcat
     1801 gtccaacctc aggatttaat ccaactaatt ctgggtggtc aagggaatta ataaatgcaa
     1861 gggcatgccc aattgtgggc aggaatatat cgccgcgagg ttcatttggt tttggctcca
     1921 ttgcaaactt aatgttgtat cccttatcaa taacaaattg accaagaata ttgaaagcct
     1981 ctcgcattct ctcaagtgct accacaggat ctttgccgat atcagtctct gctccctcac
     2041 gcccgcccca acagacatag gtatgagcgc ctagctctac agctaattca atatttgtca
     2101 ttacttttct gatggcatac ctgcgaatat ctctattgtt agaggtaaat gctccatctt
     2161 taaatactgg atggctaaat aaatttgttg ttgccatcgg caccttcatt ccattctcat
     2221 caagtgcctt cttaaagcga gcaatatgtg ctgcgcgatc tgaagttgat gaaccaaatg
     2281 gaattaagtc atcatcatga aatgtcactc catatgcccc gcgctttgct aattcgctaa
     2341 ctgtgcgaat tggatcaagt gctgctctgg tggcatctcc aaatggatcg cgggcttgcc
     2401 acccaacagt ccataggccg aatgtgaatt tatctgctgg tgttggattt ctcatgggtg
     2461 gagcctaacg attttgctca cctactagaa tacgaaagtg ctagaaaaat cagatcgacc
     2521 ttggggtcgc tatgaagtct tgcaagagag cccatctcat aaggtcaaat gcatctgggt
     2581 atctccagga aagcggcttt cctatcagcg ccatcaaaag cgctctgagc attggtttat
     2641 cgtttcagga agtgcgcagg tgataattaa tggcgtggtt tcatcaccga gtgctggcga
     2701 tagttttgat attcctgcag gagccttgca tcgaatagca aatgtcggag ataacgatgt
     2761 ggtcttcatt gaggttcaga ctggaactta ctttggcgaa gatgatattg agcgccttga
     2821 agatgacttt gggcgttgat agataaatga acgtaaaaga ttttaagatg ttttcagaga
     2881 tttctgagac ccctgaagtt ttttcaagac tgataaattc taaggcgcaa tttcaactag
     2941 ctgcagagaa aataaaagca cgagatatta caaatgtaat tatcctggca cgaggaacat
     3001 ctgataatgc tgggcactat ttgaagttct tgattgaagt taagctggga ttaccagtag
     3061 gacttgcttc accttcatct gtatcaatct atggcgctaa ggttgatttc aaaaatactt
     3121 tggtaattgc cttaagtcag agtggaagat ctcctgacct tataactttt gcgagtgccg
     3181 ctagagaagg tggagctcta ttaattgcta tgacgaataa ttcagattct ccactagcaa
     3241 aagctggtga tattcacatt gatctttctg ctggacctga agttgcagtt gctgcaacaa
     3301 agagctattc agcagagctc ctagcatcct tgcttttagt tgatagctgg atgggcaata
     3361 aggaagaagc aagggcacat ctagttgatt catccagaga gtgcatagct aacttaagcc
     3421 aggttgatag cttcgctaac tctcttgatg cggcaagaga aattgttgtt attgggcggg
     3481 gctattcata tgccaatgcc aaagaattgg cgcttaaaat tcaagaaact agctatatcc
     3541 cagttcaagg gatgtcgagc gctgattacc aacatggtcc aatagcaacc ctaaataaaa
     3601 acagccaagt aattgtctta tctccttcgg gaatgcctaa gaaagcactt gaagactcaa
     3661 tagctcgtat tcgtcagagc gggccagagc taatatggct gggaagcaat caacttgcgc
     3721 ttagggaaga gaaagtcata aaaggttcta cgcaggcaag cgaagaagag tcaacgataa
     3781 ttgatgctgc gttaattcaa tacttgactc tgagatttgc aatcaagaat ggattcaatc
     3841 cagattcacc ggcagggctc agtaaggtca ctgagaccct ttaattcgct ctctgtagta
     3901 ataagcactc gacttaggaa ttcgtttctg ggtttgataa tcaacataga tgattccaaa
     3961 cctctttgca tacccataag cccattcaaa attatcaatt aaactccagg caaagtaacc
     4021 actaattggc actccattat cttttgcggc gaacatggca tcaagatgtc gctctagata
     4081 acttactcgc ttgggatcat caactttccc atctataact tcatcatccc aagcagaacc
     4141 attttctgta atgaatattt cctttggttg atattctcta tttattcgct caaggagctc
     4201 agtaaagctt tgcgcatgaa cttcccagcc catcccagtt ctttcaacgt tgggcgcagt
     4261 tacttgctta taaggcaatg gtttagcact tgggtcgtac gccacagtct gtctgaagta
     4321 ataatttaag cccaagtaat ctaatggggt tgagataatt tccatatcac ctgggtttat
     4381 cgggacttcc ttgccgaatg ctttaacaat gtcctctgga tactttgctt taaatactgg
     4441 atcgccaaac cagcggttat caaaaccatc cgccagctct acagctaact tgtcatcact
     4501 tctttggcct agagttatcg ctggagtgaa attaagagtt ataccaactt ttagctcgct
     4561 agaaacttct ctaatcgctt ttgtggcaag gccatggcca agcaataaat gatgagaggc
     4621 attgattcca gtttggagat cctttattcc aggagccatt acgccatata gatggcccag
     4681 ccatgcgctg caaaatggct cattaattgt tatccagttt tttaatcgat ccgagaaagc
     4741 ctctgccatc ttctggctat attctgcaaa ccagtaagcg caatcacgat tattccaacc
     4801 acccttatct tgcagactct ggggaagatc ccaatgataa agagtgggcc agggggtaat
     4861 tcctcgctcg agtgcgccat caattaatct gtcgtaaaaa tccattccct ctttattaag
     4921 cgctccataa ccagagggta taacccgggg ccacgcaatt gagaagtgat aagccccaac
     4981 gcctaaccac ttcatcaaat ctagatcttc gttaaagcga tggtagtgat cacaggcaac
     5041 atcgccgtta tcaccattgg caactgctcc tggaattttt gaaaatgtat cccaaataga
     5101 tggcccacga ccgccttgat ttgctgctcc ctcgatttga tatgaagatg ttgagacgcc
     5161 ccaagtaaac atttcgcccc atttctttaa tatcacttat atctctatgg agattctaga
     5221 ggtgttgtag atacagtgtc ctttatgttc tcactaaagg gcaagagcgc tctagtcact
     5281 ggagcaggag atgcttcagg aataggtttt gcagccgcta aggcacttaa ggaactgggc
     5341 gcagaagtct ttattacttc aactt
//
LOCUS       DAOROD010000038         5155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1335_length_5155_cov_35.844118, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000038 DAOROD010000000
VERSION     DAOROD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_5155_cov_35.844118"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLW53_05730"
     CDS             <1..1019
                     /locus_tag="PLW53_05730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRD36954.1"
                     /translation="LLANYTSLHVGGPANKFVRVSSESELVEAIKAADSAGEEVLIMG
                     GGSNVLISDAGFNGTVIHVETKGNSFHIDACSGGMISVASGENWDEFVAFMIEKGFAG
                     LESMSGIPGSVGAAPIQNIGAYGHEISEVVARVRCWDRKESAYKTFSNSECQFSYRSS
                     IFKKSSGRYVVLEVTFQLRQGEMSLPITYSELANYLKVKLGERVLITELRKAVLALRA
                     AKGMLLDEKDKNSWSAGSFFINPILDAKDAERLPEGAPRWPQEDGRVKTSAAWLMENS
                     GVKKGETHNGAGISSKHVLALVNTGSASADDILELARSARKAVKDKFGILLEAEVALV
                     GLNL"
     gene            1016..1501
                     /locus_tag="PLW53_05735"
     CDS             1016..1501
                     /locus_tag="PLW53_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HRD36955.1"
                     /translation="MRKLFLSLLLIFLPLSPSYSHSSLVSASPSSGSVLVDFPMEIVL
                     DFNEELLVIGQDDPNSIEVFDAAGELVSGDSKVSGARISAGLSINGNGSYSVKYRVVS
                     KDGHVIEDSYDFEVQSPIAISAPIKEEVVSIPSSSRIPITSALVLALVIGLITLIKRR
                     K"
     gene            complement(1502..2698)
                     /locus_tag="PLW53_05740"
     CDS             complement(1502..2698)
                     /locus_tag="PLW53_05740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRD36956.1"
                     /translation="MSKPRISSRIAAIAESATLAVDAKAKALKAAGRPVIGFGAGEPD
                     FPTPDYIVEAAAAATKVVANHRYSPTPGLPELRQAIVNKTKRDSNYEISVDQVLVTNG
                     GKQAVYQAFASIINPGDEVILPSPFWTTYPECIKLAGGKAIEVFADESQDYLVSIEQL
                     EAARNENTKALLFCSPSNPTGSVYSVEQVKAIGQWAAKHGIWIISDEIYEHLLYDGAS
                     APSIPVIVPEMADKTIIINGVAKTYAMTGWRVGWMIGPKDVIKAATNLQSHLTSNVSN
                     VAQRAAIAALDGDLSAVKKMGEAFDRRRKLIVKLLNEIPGISCPMPKGAFYAYPSVKG
                     VLNKEINGRTPTTSAELATIILEEAEVAVVPGEAFGPSGYIRLSYATSDEDIIEGVTR
                     IKKLLS"
     gene            2875..2948
                     /locus_tag="PLW53_05745"
     tRNA            2875..2948
                     /locus_tag="PLW53_05745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2909..2911,aa:Trp,seq:cca)
     gene            2999..3220
                     /gene="secE"
                     /locus_tag="PLW53_05750"
     CDS             2999..3220
                     /gene="secE"
                     /locus_tag="PLW53_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRD36957.1"
                     /translation="MSENSESKELGFFSRIALFYRQVINELRKVVWPSRNMLTTYTGV
                     VLVFVAFVIAVVSVFDLGLTKLVFFIFGE"
     gene            3234..3914
                     /gene="nusG"
                     /locus_tag="PLW53_05755"
     CDS             3234..3914
                     /gene="nusG"
                     /locus_tag="PLW53_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRD36958.1"
                     /translation="MSEEQLTATTDEVVASDESVDTDIELEEEEVQEIVSPEVAAFRA
                     ELAAKSGDWYVVHSYAGFEKRVKGNLMQRITSLHMEDFIFEIQVPEEEVTEIKNGQRK
                     QVKRNVYPGYVLVRMDLSDESWSCVRNTPGVTGFVGNSHHPSPLPLSEAEKMLMPREL
                     KKTMKPDIKVVDFTVGESVTVMDGPFATLPASISEIMPEQAKLKVLVSIFGRETPVEL
                     SFHQVQKI"
     gene            3944..4372
                     /gene="rplK"
                     /locus_tag="PLW53_05760"
     CDS             3944..4372
                     /gene="rplK"
                     /locus_tag="PLW53_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRD36959.1"
                     /translation="MAPKKKVQALIKLQIQAGAATPAPPVGPALGQHGVNIMDFVKQY
                     NAATEKQRGEIVPVEITVYEDRSFTFITKTPPASRLLLKAAGLEKGSSVPHKDKVGSV
                     TWKQCEDIATLKKDDLNANDVKAGAKIIAGTARSMGIIVN"
     gene            4439..5149
                     /gene="rplA"
                     /locus_tag="PLW53_05765"
     CDS             4439..5149
                     /gene="rplA"
                     /locus_tag="PLW53_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRD36960.1"
                     /translation="MKRSKKYTAAAAKINQDQLYTPLAGMKLVKETNVTKYDASVEVS
                     MVLGVDPKKADQAVRSVVNLPHGTGKTARVLVFATGPRAEEAKAAGADIVGGDELIEE
                     VNKGRLDYDAVVSTPELMGKVGRLGKVLGPRGLMPNPKTGTVTTDVAKAVGDIKGGKI
                     EFRVDKDCNLHFIIGKSSFSAEALADNYAAAFDEILRAKPASSKGRYIRKAVVSTTMG
                     PGVTLDPSYAREGGPAIE"
BASE COUNT         1470 a         1018 c         1247 g         1420 t
ORIGIN      
        1 agctacttgc caattacacc agccttcatg ttggagggcc ggcgaataaa tttgttcgcg
       61 tatcaagtga gagcgagcta gttgaagcta ttaaagctgc agatagcgct ggtgaagaag
      121 tattaataat gggcggcgga tctaatgttt tgatctctga tgctggattt aacggcacag
      181 ttattcacgt tgaaacaaag ggaaacagct ttcatatcga tgcctgtagc gggggaatga
      241 tcagcgttgc atcgggtgag aactgggatg aatttgtcgc atttatgatt gaaaaaggat
      301 ttgctggtct tgagagtatg tctggaattc caggaagtgt gggagctgct ccaattcaaa
      361 atatcggcgc ctatggccat gagatatcag aggttgtggc aagagttaga tgctgggata
      421 ggaaagagag cgcttataag actttctcta actcagagtg tcagttttct tatcgctcat
      481 cgatatttaa gaaaagctct ggtcgatatg tagtgcttga agtaactttc caattaagac
      541 agggcgagat gagccttcct attacttata gcgagcttgc aaattatcta aaggttaaat
      601 taggcgagcg agtattaatt acagagctaa gaaaagcagt attagcttta agagctgcta
      661 agggaatgct ccttgatgag aaagataaaa atagctggtc tgctggttct ttctttatca
      721 atccgatttt agatgcaaaa gatgcagaaa ggcttccaga gggcgcgcct agatggcccc
      781 aagaagatgg gcgagtaaaa acttctgctg cctggcttat ggaaaattct ggagtgaaaa
      841 aaggtgagac tcataatggc gcaggtattt caagtaagca tgtattagca cttgttaaca
      901 ctggaagtgc tagcgcagat gacatattag agctagctag aagtgcaaga aaggcagtaa
      961 aggataagtt tggaatcttg ctagaggcgg aagttgctct agttggctta aatctatgag
     1021 aaagctcttt ctctcactcc ttcttatctt tcttccgtta agcccttctt atagccatag
     1081 ctctttagtt agcgcgagtc caagtagtgg atctgtatta gttgattttc ctatggagat
     1141 agttcttgat tttaatgaag agctattggt aattggccaa gatgatccca atagcattga
     1201 agtctttgat gcagctggag aattagtttc aggagattca aaagtatctg gggcaagaat
     1261 tagcgcagga ctttctatta atggcaatgg aagttatagc gttaagtatc gagtggtttc
     1321 aaaagatggc catgtcattg aagatagcta cgactttgaa gttcaatcac cgattgcgat
     1381 ctctgctcca attaaagaag aggtagtttc tattcctagt tcctcgcgca ttccaataac
     1441 ttcagcactt gttcttgcgc tagttattgg gctaataact ttgattaaga gaagaaagta
     1501 actagctaag tagcttcttt attcttgtca cgccttcaat gatgtcttca tctgatgtgg
     1561 cataagagag cctgatatat ccagatggtc caaaggcctc acctggaaca actgctactt
     1621 cggcctcttc caatatgatc gtagcaagct cagcagaagt agttggagtg cggccattaa
     1681 tctctttgtt aagaacacct ttaacagacg gataagcata gaaagctcct tttggcatcg
     1741 gacaggaaat tcctgggatt tcatttagaa gttttacgat tagctttctt cttctatcaa
     1801 aagcctcacc catcttctta actgcgctta aatcaccatc gagcgcggca attgcagctc
     1861 tttgtgccac atttgaaaca ttagatgtta gatgtgattg cagattggta gcggctttga
     1921 ttacatcctt tggcccaatc atccaaccaa ctcgccatcc tgtcatggca taggttttag
     1981 caacgccatt aataataatt gtcttatcag ccatttcagg aacgataact ggaattgaag
     2041 gagcgctagc tccgtcatag agcaggtgtt cataaatctc atcgctaatt atccagatgc
     2101 catgctttgc ggcccactga ccaattgctt taacttgctc gactgaatag acagatcctg
     2161 ttggattaga gggggagcaa aagagcagcg ctttagtatt ttcatttctt gctgcttcaa
     2221 gctgttcaat acttactaaa taatcttgtg attcatcggc aaatacttca attgcttttc
     2281 cgcccgctaa tttgatgcac tctggatatg tggtccaaaa aggtgagggg agaattactt
     2341 catcgccagg attaataatg gaagcaaatg cttgatagac cgcttgcttg ccgccatttg
     2401 ttactagaac ttgatcaacg ctaatttcat aatttgaatc acgctttgtt ttattaacaa
     2461 ttgcttgacg aagttcagga agtcctggag ttggtgagta gcgatgatta gcaactactt
     2521 ttgttgcagc agctgctgcc tcaacaatgt aatccggagt tggaaaatct ggttcgcctg
     2581 cgccaaagcc gatcaccgga cgaccggcag ctttaagggc ctttgctttg gcatcaacgg
     2641 ctaaggtcgc cgactctgcg atagcggcaa ttcttgaaga gattcgaggc ttactcatag
     2701 gcggtaagtc tttcataggc gcaggtgagc gcgagttcta ccccttttca tgcgggtttt
     2761 acctattggg tagacctgac ctacacttgc ccatcctggc gcttgcttga tcgggtaatt
     2821 aaaaaaatct gattatgaaa agtccatgct ttgtcatgtt cgaaagcgcc atatagggca
     2881 gtagctcaac tggccagagt tccggtctcc aaaaccggcg gttgggggtt caagtccctc
     2941 ctgccctgcg cagttgtaag aagtaagaag ttaacgaaag agagaggaag gatgcaaaat
     3001 gagtgagaac tcagagagta aagaattagg tttcttttct cgcatcgccc tgttctatcg
     3061 ccaggttatt aatgagctac gtaaggtggt ctggccatct agaaatatgt tgaccacata
     3121 taccggagta gttcttgtct tcgtggcctt tgttatcgca gttgtttcag tatttgatct
     3181 aggtttaacc aaattagttt tcttcatatt cggcgagtag gtaaggaggg atgatgagcg
     3241 aagagcaatt aactgccact actgatgaag tagtagcaag tgatgagtca gtagatacag
     3301 atattgaact tgaagaggaa gaagttcaag agatcgtatc tcctgaggtc gccgcattcc
     3361 gcgccgagtt agcagcaaag agcggggatt ggtatgtcgt ccactcttat gctggttttg
     3421 aaaagcgagt aaagggcaac ctgatgcagc gcatcacctc acttcatatg gaggatttca
     3481 tctttgaaat ccaagtccct gaagaagagg taaccgagat taaaaatggt cagcgcaagc
     3541 aggttaagcg caatgtctat cctggctatg tattagttcg tatggatcta tctgatgaat
     3601 cttggtcctg tgtgagaaat actccaggag taactggctt tgttggaaac tctcatcatc
     3661 caagcccgct gccgctatct gaggcagaga agatgctgat gccaagagag cttaagaaga
     3721 ctatgaagcc tgatattaaa gttgttgatt tcactgttgg tgagtcagta actgttatgg
     3781 atggcccttt tgccactctt cctgcatcga tctcagagat catgccagag caggcaaagc
     3841 taaaggtttt ggtttcaata tttggccgtg agactccggt tgagctctca ttccaccaag
     3901 ttcagaagat ttaaagagaa aaggtaaaaa aggggagaag aagatggcac cgaagaagaa
     3961 agtccaagca cttatcaagt tgcagatcca agctggagct gccacacctg ccccaccagt
     4021 gggccctgcc ctaggtcagc atggtgtgaa catcatggac tttgttaagc aatataacgc
     4081 tgccactgag aagcaaagag gcgagatcgt tccagttgag attactgttt atgaagatcg
     4141 ctcatttacc tttattacta agactcctcc tgcatcacgt ctacttctta aagcagcagg
     4201 gctagaaaaa ggatcttcag ttccacataa ggataaagtc ggatcagtta cttggaagca
     4261 atgtgaagat attgcaacct tgaaaaagga tgacttaaat gccaatgatg tgaaagctgg
     4321 agcaaagatc attgcaggaa ctgcaagatc aatgggaatc atcgtcaact aaaaaataag
     4381 tcgtgggagg accgcgctgg tccgctacta ccactgcttc caaacaaagg aaaaagatat
     4441 gaagcgcagt aagaaataca ccgcagcagc ggcgaagata aatcaagatc agctctacac
     4501 accactagct ggtatgaagc tggttaaaga aactaatgtc accaaatacg atgcctcagt
     4561 tgaggtttca atggttctcg gcgttgatcc taaaaaggcc gatcaagcag ttcgctccgt
     4621 ggttaatttg ccacatggaa ccggtaagac tgccagagtt ctagttttcg caactggacc
     4681 aagagcagag gaagccaagg cagcgggagc tgacattgtc ggcggcgatg agttaattga
     4741 agaagttaat aagggccgtc ttgattatga cgcagttgtt tcaacccctg agttaatggg
     4801 caaagttgga cgtcttggaa aggttctagg accacgtggt cttatgccaa atccaaagac
     4861 cggaactgtc acaactgatg tggcaaaggc tgttggtgat atcaagggtg gaaagattga
     4921 attccgcgtt gataaagatt gcaacctaca tttcattatt ggtaagtcct catttagcgc
     4981 agaggcgcta gctgataact atgcagctgc attcgatgag atcttgcgag caaagcctgc
     5041 atcatcaaag ggtcgctata ttagaaaagc agttgtctca accactatgg gacctggcgt
     5101 aactcttgat ccaagctatg cccgtgaagg tggaccagca atagagtaag taata
//
LOCUS       DAOROD010000039         4794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1537_length_4794_cov_34.739396, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000039 DAOROD010000000
VERSION     DAOROD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_4794_cov_34.739396"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1227
                     /locus_tag="PLW53_05770"
     CDS             100..1227
                     /locus_tag="PLW53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRD36961.1"
                     /translation="MSLKKTLTAAAVALTSFALVISGTTSASAAKVPSSKAKAGDECG
                     RAGMVAKARGVEGSNLTCTKVTVGTAAGSLRWWYPDLKPLTTIDWTVPAGPGGYSATT
                     DAISKTLIAEGLLTTSTTDFKPGAGGTTGLAHFQTIKGKQNSALIIGIALTGGMAQVA
                     NKNTSDLLASVPIARVMREYNAIFVPANSKYKTLNQFVNDVNTNGRAIAVSGGNVGGV
                     DHQVIGALLKTANVPISKLNYVVGTGSEPLTKVLSGAAPVGVIGIVDVLPYVADNRLR
                     ILGISSPKPIAGVKGKTFIQQGYNLVYGNWRGVMAPADISEANRLNFVKVMDVMRSTD
                     TWKQVLATNKWYDEFAGGKDFESFLKTHIPQIKAFIAEIGL"
     gene            1245..1763
                     /locus_tag="PLW53_05775"
     CDS             1245..1763
                     /locus_tag="PLW53_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD36962.1"
                     /translation="MIKVTRAVLAEAIFALAFLLLGLFVLYDAFTLEEPGVYSVVSPK
                     TFELIIGFFTTFVALLLIIQVLRGKLGIAEGTEAGAAQLRPDVKTMSILLASILIHIV
                     LLEPAGYIAAASLTFFGVAYAFGSRKFLKDILISFVFAVVVYFVFSKGLRIFLPDGIF
                     ENLLGLAREVEG"
     gene            1764..3269
                     /locus_tag="PLW53_05780"
     CDS             1764..3269
                     /locus_tag="PLW53_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD36963.1"
                     /translation="MGNLDLLLEGFSNALTAQNLLYGLLGTFLGTLVGVLPGIGPALA
                     IALLLPVTLSVDPTSALIMFAAVFYGAMYGGSTTSILLNTPGEAGSVITSVEGHKMAR
                     NGRAGAALTTAALGSFVAGLIATIMLAAAAPALAELALKVQPAGYVALIVFAFATVGT
                     LLGSSRVRGLVSLSVGLIFGLVGADQQTGINRLSFGIQEATDGLEVIVVIISFFALGE
                     VIYYASRFQDKSWQVLPVKGKVWMDREDFRRSWKPWLRGTAIGFPMGIIPAGGSEIPT
                     FISYSVEKKLSKNKEEFGNGAIEGVAGPEAANNANAAGVLVPLLALGLPTSATAAIML
                     VAIRNFNIQPGPMLFEMNAPLVWTLIASLFIGNTLLLLINLPLIRIWIQLLHIPRHYL
                     YAGITTFGLLGAYSLNNSTFDLRLAFVLGFVGYLFRRFGIPNTPLVLGVILGPVVEIQ
                     FRQAVQLSNGDYSILVSGLLPKIIYLTLILVLFAPALMKLVKGKAGQGLRK"
     gene            3266..4450
                     /locus_tag="PLW53_05785"
     CDS             3266..4450
                     /locus_tag="PLW53_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD36964.1"
                     /translation="MTSRSTNSWLWALLINVALAQASIYVMRPMITYRALENGASGYE
                     IGLIASIYALLPLLIAVPMGRWVGRFGEIPLLFIGCFSFIILGVAFAFLNNFIAIAAA
                     TALAGVAHLSNVAASQAMVANRSAMNLQDQNFGYFSFATSLGHSVGPILGGLIAGSAG
                     VLPRSSTSAFIFAALLALLSLVPFFLFKSIKEVRTQQERDAAGAIKARDVLSRPGIKP
                     AIWTSLSVAAANDVLVVILPLVGTEKGISPVVIGAILSIRSAAAMISRFLLGRLTKSY
                     GSARTMNYSIFFSALFLFASIYANSSIALCLAMAIVGFLLGIGQPLTMSIVSKKTPSQ
                     ERAMAISIRLFGNRIGQFLVPLAAGALAAPFGASSVFIGLGVLVASAGVVSAATLDDK
                     TA"
     gene            complement(4447..>4794)
                     /locus_tag="PLW53_05790"
     CDS             complement(4447..>4794)
                     /locus_tag="PLW53_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRD36965.1"
                     /translation="WQPPSYVFGIIWPYNFMVLGIASYQVSQSLTKSENILWLVFFGL
                     SVAAALTWAYQFYVPHNLTLAPIALTLCALLTLPVLYLTFKATLLMGLLLVPYQIWVA
                     IAASLAWGYSTRN"
BASE COUNT         1266 a          989 c         1080 g         1459 t
ORIGIN      
        1 tgttatcaat ctgaggctga aaacgccttc tctcaagcta tcggggccga tacggtaggg
       61 ctcttctctc accctcgaga gaaaaaaata ggagaactaa tgtctttgaa aaaaacactt
      121 actgcggcag cagtagcgct aacttcattt gcgctagtta tatctggaac aacttcagcg
      181 agtgctgcaa aagttccatc atcaaaagca aaagctggag atgagtgcgg acgcgctgga
      241 atggttgcta aggctcgcgg cgtagagggc tcaaacctca cctgcaccaa agttaccgtt
      301 ggaacggctg ctggcagcct tcgttggtgg tatccagatc taaagccgct aaccacaatt
      361 gattggacag tacctgcagg tcctggagga tactcagcaa caactgatgc aatttcaaaa
      421 actctaattg cagaaggttt gctaactaca agcaccacag acttcaagcc tggtgctggt
      481 ggaactaccg gtcttgcaca cttccagact attaagggca agcaaaattc agctcttatt
      541 attggtattg ctctaactgg cggtatggct caggtagcaa ataagaacac atcagatctt
      601 cttgcctctg ttccaattgc gcgagttatg cgcgaataca acgcaatctt cgttccagct
      661 aattcgaagt acaagacttt gaatcagttt gtaaatgacg ttaacaccaa cggtcgcgca
      721 attgcggtct caggtggaaa tgtcggcggc gttgatcacc aggtaattgg tgctcttctt
      781 aagaccgcta atgttccaat ttccaagctc aactatgttg ttggtaccgg atcagagcca
      841 cttactaagg ttctaagcgg agcagcccca gttggagtta tcggaatcgt tgatgttctg
      901 ccatatgttg cagataacag actacgcatt cttggaattt catcccctaa gccaattgct
      961 ggcgtgaagg gcaaaacctt tattcagcag ggctataact tggtttatgg aaactggcgc
     1021 ggagttatgg caccagctga tatcagcgag gctaatcgcc ttaacttcgt aaaggttatg
     1081 gatgtaatgc gttctaccga tacttggaag caagttcttg caaccaataa gtggtatgac
     1141 gaatttgctg gcgggaagga ttttgaatcc ttcttaaaga cccacattcc acagataaag
     1201 gcatttatcg ctgaaatcgg tctataaaaa aacttaataa gtagatgatt aaagtaaccc
     1261 gagcagtact tgctgaagcg atttttgcct tagcatttct actgctcggg ttatttgttt
     1321 tgtatgacgc ctttacttta gaagagcctg gggtttactc agtcgttagc ccaaagactt
     1381 ttgagttaat aattggtttc tttacaactt ttgtagccct acttctaatc attcaagtct
     1441 tgcgaggaaa gctaggaatc gcagagggca ctgaagctgg agctgctcag ctacgtcctg
     1501 atgtcaaaac gatgtcaatt cttcttgcct caattctgat tcatatcgtg ctgcttgagc
     1561 ctgccggata tatcgcagct gcttcgctaa ctttctttgg agtggcatat gcatttggat
     1621 ctagaaagtt tttgaaagat atattaattt cctttgtttt tgcagtagtt gtctacttcg
     1681 tcttttctaa aggtttgaga atcttcttgc ccgatggaat ttttgaaaac ttacttggcc
     1741 ttgccagaga agttgaggga taagtgggaa atctagattt attacttgaa ggcttttcaa
     1801 atgcgctaac agcgcaaaat ttactttatg gcttacttgg aacctttctt ggaactctag
     1861 ttggagttct gccaggaatt ggtccagccc ttgctattgc acttcttctg cccgtcacgc
     1921 tttcagttga tcccacatca gctttaatta tgtttgctgc cgtcttctat ggagcgatgt
     1981 atggaggatc tacgacctct attttgctta ataccccagg tgaggccggc tcagtaatca
     2041 cttcagttga aggccataag atggcaagaa atggcagagc tggagcagct cttacaaccg
     2101 cagcacttgg ctcatttgtg gcaggtctga ttgcaacaat aatgttggca gcagcagctc
     2161 ctgcgctagc tgaacttgcg ctaaaagttc aacctgctgg atatgtagct ttaattgtct
     2221 ttgcctttgc aacagttgga actcttcttg gatcatcaag ggttagaggg ctagtttcat
     2281 tatctgttgg cttaattttt ggacttgttg gagctgatca acaaactgga atcaatagac
     2341 tttcatttgg aattcaagaa gcaacagatg gccttgaagt aattgttgtc atcatttctt
     2401 tcttcgcact tggtgaagtc atttactatg caagtcgttt tcaagataag agttggcagg
     2461 tgcttccagt taaaggaaaa gtttggatgg accgtgaaga tttccgtaga tcctggaagc
     2521 cttggctgag aggaacggca atcggatttc ctatgggaat tatccctgct ggtgggtcag
     2581 agattccaac ttttattagc tactcagtgg agaagaagct atcgaaaaat aaagaagagt
     2641 ttggaaatgg cgctattgaa ggagttgcag gccccgaggc agcaaataat gccaacgcgg
     2701 caggagttct agttccactt cttgcgctag gccttccaac ttcagcaaca gctgcaatta
     2761 tgttggttgc gataagaaac tttaatatcc aacctggccc aatgctcttt gagatgaatg
     2821 ctccactagt ttggacacta attgccagtt tatttattgg aaatacactt cttctgctaa
     2881 ttaatttgcc tctaattaga atctggattc aacttcttca tattccaagg cattatctat
     2941 atgccggaat tacaaccttt ggccttttgg gcgcttatag cctaaataac tccacatttg
     3001 atctgcgttt agccttcgtt ctgggctttg tcggatatct cttccgtaga tttggaattc
     3061 caaatacccc attagttctg ggagtaattc tgggtcctgt tgttgagatt caattccgcc
     3121 aagcagttca gctaagtaat ggcgactatt caattctcgt aagcgggtta ttacctaaaa
     3181 ttatttactt gactctgatt cttgttttat ttgcacctgc acttatgaag ttagttaaag
     3241 gtaaggcggg tcaaggctta agaaaatgac cagccgttct acaaatagtt ggttatgggc
     3301 tcttcttata aatgttgccc tagcgcaagc aagtatctat gtgatgcgcc cgatgattac
     3361 ttatcgagct ttggaaaacg gagcatctgg atatgagatt ggtctaatag cctctattta
     3421 tgctctgctt cctttattaa tagcagttcc gatgggcaga tgggtcggaa gatttggaga
     3481 aatcccgctg ctatttattg gttgtttctc tttcataatt cttggagttg cctttgcttt
     3541 ccttaataac tttattgcaa ttgctgctgc cacagctctt gctggcgttg ctcatctttc
     3601 aaatgttgca gcaagtcaag caatggttgc taatcgaagc gctatgaatc ttcaggatca
     3661 aaactttgga tatttctctt ttgcaacatc acttggtcat agtgtggggc caattcttgg
     3721 aggtttaatt gcaggatctg caggagtctt gccgcgctct tcaacctctg cctttatatt
     3781 tgcagcgctc ctggccctct tatcacttgt tcccttcttt ctctttaaat caataaaaga
     3841 ggtgcgaact cagcaggagc gagatgcagc gggagcaatt aaagccagag atgttctctc
     3901 tcgccctgga atcaaacctg ctatctggac ctcgctatct gtggctgcag caaatgatgt
     3961 attggttgtg attttgcctc ttgtgggaac agagaaaggc attagtcccg ttgtaattgg
     4021 agcaattctc tcgattcgct cagcagcagc aatgatttca agatttctac taggtcgatt
     4081 aacaaagagc tatggatctg caaggacgat gaattacagc atcttcttct cagctctatt
     4141 cttgtttgct tcaatctatg ccaatagttc aattgcactt tgtttagcta tggccatagt
     4201 tggattctta ctaggtattg gccagccatt aactatgtcg atagtttcaa agaaaactcc
     4261 atctcaagag cgagctatgg caatatcaat tagattattt ggtaatcgaa ttggccagtt
     4321 tttagttcct cttgcagcag gagcccttgc tgcgccattt ggagcaagct cagtctttat
     4381 cggcctagga gtattagtag catctgctgg cgttgtatct gctgcaactc ttgatgataa
     4441 aactgcttaa ttgcgagtgg agtatcccca agcaaggctt gctgcaattg caacccagat
     4501 ttgataagga acaagaagta gtcccattag tagcgttgct ttaaatgtta gatagagaac
     4561 tgggagagta agaagagcac ataaagttaa agcaattgga gctagggtga gattatgagg
     4621 aacatagaat tgataagccc aggtaagggc ggcagcaaca cttaaaccaa agaaaacaag
     4681 ccataagatg ttttcagatt tagttaatga ttgtgagact tgataggaag caattcctaa
     4741 aaccataaag ttatagggcc agatgattcc aaatacatag cttggcggct gcca
//
LOCUS       DAOROD010000040         4194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_1949_length_4194_cov_32.192317, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000040 DAOROD010000000
VERSION     DAOROD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_4194_cov_32.192317"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..749
                     /locus_tag="PLW53_05795"
     CDS             21..749
                     /locus_tag="PLW53_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRD36966.1"
                     /translation="MKAEKLSQERLLALQDLDSSLMQLEHKANNLPLSKLLDEKRLEF
                     ASARDLAVAASTERSDIKHELSKSELDVEQVLSRIEKDEKRLASGLGTPKELEQTQHE
                     LESLNKRRAELEDIELEVMVRLEGLDSRIAELSKRRDELAIEVDAITKDRDQALEQIS
                     QAMSATKSDRDALAKEIEAELLSLYEKIRSTSDGLAAVRLFDGKCQGCHLTMSAAELT
                     RINSLAIDELVRCEECRRILIRIT"
     gene            750..1151
                     /locus_tag="PLW53_05800"
     CDS             750..1151
                     /locus_tag="PLW53_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HRD36967.1"
                     /translation="MARKLIIYADGGSRGNPGQAAYGALVCEGDKVLVEIAEKIGIAT
                     NNVAEYQGLIAGIRAANKIDPAAQIEARLDSKLVVEQMSGRWKIKNIPLAKLAMEAKK
                     IHSLELITFTWIPREENYEADRLVNLALDGY"
     gene            complement(1155..2537)
                     /locus_tag="PLW53_05805"
     CDS             complement(1155..2537)
                     /locus_tag="PLW53_05805"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRD36968.1"
                     /translation="MISAQDLVEKILAKATSDDCIVLVKDKTQANLRWASSTLTTNGV
                     IATRSVTVIAFIATGSSMASGGVTRTSVEESDIDSILAEALAAAKASGEADDYAPLAK
                     DVSYGNWSDKHAPTGPEVFSKFAPDLGSMMQASKSDGIELFGYAEHTHESIWVGSKGG
                     MRLRYDSPVGRVEMTGKSHGRSRSTWSGVETHDFKDVSIERIDKEIRQRLNWQERKVD
                     LPAGRYTSIVPSGAVADLFTYMMWVSSGRDAFEGQSVFSKKGGGTRVGEKLSNKSLQL
                     YSDPKDKQLPGSPFVSAAVSSPFTSVFDNGQEAKRVDWIKDGVLTSLLQTRASAKLTS
                     LPYTPVGDNLIMKVDGASGNLEDLIKSVDNGLLLTTLWYIRMVDPNTLLLTGLTRDGV
                     YHVKGGEVVGATNNFRWNDSPVSALNRIAQASESAWTQPREWAGDITSMKAPALVIND
                     FNMSTVSPGS"
     gene            complement(2534..4036)
                     /locus_tag="PLW53_05810"
     CDS             complement(2534..4036)
                     /locus_tag="PLW53_05810"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRD36969.1"
                     /translation="MANTVDADFLALPLSALSDAAIDAAKAAGASHVDVRIERTRTGY
                     LSLRDAKPETQSDESNLGIGVRVIVNGAWGFASSPDLSSDTAKKLALSAVAMAKTSKP
                     LSTDEIKLVSEPVYPNKTWVSSYKIDPFLISDSEKKDRLASLSSKLLASSSVNHTSAH
                     TMFVKEQKHYADSAGTSTTQQRVRVQTQIEAISIGSHGFEAMRTLAQPAGYGWEWMDN
                     SIWDWDSEIAELPSLLAEKVASPSVEPGRYDLLIHPSNLWLTIHESIGHATELDRAIG
                     YEANYAGTSFATPDKLGNLKYGSPLMNVTGDRITEHGLSTVGFDDEGVAAQSWDIVKD
                     GILVGYQLDRRIAAAVNKERSNGCAYADSPSHVPIQRMPNVSLKADPNGPDLKGLIES
                     IDDGIFILGDKSWSIDMQRYNFQFTGQRFHRIKGGKIVGQLKDVAYQATTTDFWGAMK
                     KVGGPSTYVLGGAFNCGKAQPGQVAAVSHGCPAAIFEKINILNTVSEVGK"
BASE COUNT         1187 a          941 c          939 g         1127 t
ORIGIN      
        1 tagcctaaag tggggaagtg atgaaagctg agaaattatc ccaagagcgc ctccttgcgc
       61 tccaagatct tgattcatct ctcatgcaac tcgaacacaa agctaataat ctgcctctct
      121 caaagctcct tgatgaaaag cgcctggagt ttgccagcgc aagagattta gcagttgctg
      181 ccagtactga gcgaagtgat atcaagcatg agctttcaaa atcagagctt gatgtggagc
      241 aggttttatc tcgcattgaa aaagatgaaa agcgtctggc atcaggtctt ggaaccccga
      301 aagagctaga gcagactcaa catgaattgg aatcactcaa taaaagacga gcagagctag
      361 aagatattga acttgaagtg atggtgcgcc tagaaggcct tgatagcaga atcgctgagc
      421 tatcaaagag acgtgatgaa ttagccattg aagttgatgc aataacaaaa gatcgcgacc
      481 aagcacttga gcagattagc caagcgatga gcgctacaaa atcagatcga gatgctctag
      541 ctaaagagat tgaggcagag ctcctatcgc tctatgagaa aattagatca acaagtgatg
      601 gccttgcagc agttcgactc tttgatggaa agtgccaggg ctgccactta actatgagcg
      661 cagcagagct aactaggata aattcactag caattgatga gttagtgcgc tgtgaagagt
      721 gtcgcagaat cctcataagg attacttaaa tggcaagaaa gttaataatt tatgcagatg
      781 gtggctcaag aggaaatcca gggcaagcag cctacggagc tctagtctgt gagggcgata
      841 aagtcttagt tgagattgca gagaaaatag gaatagccac aaataacgtt gctgaatatc
      901 aaggtctaat tgctggaata agagcagcta ataaaatcga tcctgcagcc cagattgagg
      961 ctcgccttga tagcaagttg gttgtggagc agatgagcgg gcgatggaag attaaaaata
     1021 ttccactagc aaagcttgca atggaggcaa agaagattca ctctcttgag ttaattacct
     1081 tcacctggat cccaagggaa gaaaactacg aagcagatcg cttagtaaac cttgctttag
     1141 atggttatta agctctagct tccagggcta accgttgaca tattaaagtc attgatgaca
     1201 agtgctggag ctttcatact tgttatatct ccagcccact ctcttggttg ggtccaagcg
     1261 ctctctgatg cttgagctat tcgatttagc gcgctaactg gagagtcatt ccatctaaag
     1321 ttatttgtgg ctcctacaac ttcgccgcct ttgacgtgat aaaccccatc tctagtcagt
     1381 ccagttagca acaaggtatt tggatcaacc attctgatat accagagggt ggttagaaga
     1441 aggccgttat caacgctctt gattagatcc tcgagatttc cacttgctcc atcaactttc
     1501 ataatcaagt tatcgccaac tggggtgtag ggcagagatg ttaatttagc gctggctctt
     1561 gtttgcaaga gtgaggtaag aactccatct ttaatccaat caactctctt tgcctcctgc
     1621 ccgttatcaa agactgaggt aaatggcgag ctaacagcag cagaaacaaa aggagatcca
     1681 ggtagctgtt tatcttttgg atcgctatag agctgcaggc tcttattaga tagcttctca
     1741 ccaactctgg ttccgccacc ttttttggaa aatacgctct gtccttcaaa tgcatcacgg
     1801 ccgcttgaaa cccacatcat gtaggtgaag agatcagcta ccgcgcccga aggaacgatg
     1861 ctggtatatc gcccagcagg aagatcgact ttgcgctcct gccaattaag tctctgacga
     1921 atctctttat caattctttc aattgataca tctttgaaat catgggtttc aacaccagac
     1981 caggtagatc ttgagcggcc atgagacttt cctgtcatct caactcttcc aacaggtgag
     2041 tcataacgaa gtctcatccc gcctttagag ccaacccaga tgctctcatg ggtatgttcg
     2101 gcatatccaa ataattcaat gccatctgac tttgaagcct gcatcattga gcctagatca
     2161 ggagcaaatt tagaaaatac ctctggacca gttggagcgt gtttatcact ccagtttcca
     2221 tagctaacat ctttagcaag tggggcgtaa tcatctgcct cacctgatgc cttagcagca
     2281 gctaaagctt cagcaaggat tgaatcgata tcactttctt caacacttgt cctagtaacc
     2341 ccgcctgatg ccattgatga tcctgtagca ataaaggcaa tgacagttac agaccttgta
     2401 gcaattactc cattggtagt tagcgtgcta ctggcccagc gaagatttgc ttgagtctta
     2461 tcttttacta aaacgatgca atcatcactg gttgccttag caagaatctt ttcaactaaa
     2521 tcttgagcag agatcatttg cccacctcag atacggtatt tagaatgttt atcttctcaa
     2581 agatagctgc tgggcagcca tggctaacgg cggcaacttg tcctggttgc gcctttccac
     2641 aattaaatgc gccacctaga acataagttg atgggccgcc aactttcttc attgcccccc
     2701 agaaatcagt ggtggttgct tgataggcca catcttttag ttgacccaca atctttccgc
     2761 ctttaatgcg atgaaaacgt tggccagtaa attggaagtt atagcgctgc atatcaattg
     2821 accaggactt atcaccgagg atgaagatgc catcatcgat agattcgatt agtcccttta
     2881 gatcagggcc atttggatca gcttttagtg agacattagg cattctctga atcggcacat
     2941 gagatggtga atctgcatag gcgcagccat tgctccgttc cttattaaca gccgcagcaa
     3001 ttcttcgatc taattgataa ccaaccagaa ttccatcttt aacgatatcc cagctctgag
     3061 cagcaacacc ttcatcatca aagcccacag tgcttagacc atgctcagtg atgcgatctc
     3121 cagtgacatt cataagaggg gagccgtatt tcaagttgcc tagcttgtct ggagtagcaa
     3181 aagaagttcc cgcgtaattt gcttcatatc caattgcacg atccagttct gtggcatgac
     3241 caattgattc atgaatagtt agccagagat ttgaaggatg aattagtaag tcgtatctac
     3301 caggctcaac agatggtgag gcaactttct ctgcaagaag ggatggaagc tctgctatct
     3361 cactatccca atcccagatt gagttatcca tccactccca gccatatccg gcaggttgtg
     3421 caagagttcg catcgcttca aaaccatggc tgccaattga gattgcttca atctgggtct
     3481 gcactctaac tctctgctgg gttgtgctgg tgccggcaga gtctgcgtaa tgcttctgct
     3541 ctttaacaaa catcgtgtga gcgctggtgt gattaacgct agaggatgcc agaagctttg
     3601 agctaagtga tgccaggcga tctttcttct cgctatcgga gataagaaat ggatcaatct
     3661 tgtatgagga gacccaggtt ttattgggat agaccggctc actaactaat ttaatttcat
     3721 ctgttgagag cggctttgaa gtcttagcca tagcaactgc gcttaaagct aatttcttag
     3781 cagtatcact tgagagatca ggagatgaag caaagcccca agcgccatta acaattactc
     3841 gaacgccaat tccaagattt gattcatcgc tctgtgtctc aggctttgca tctcttagtg
     3901 agagatatcc agttctagtt ctctcaattc tgacatcaac atgagatgca ccagcggcct
     3961 ttgctgcatc aatagctgca tcagagagag cgcttaaagg aagtgctaga aagtctgcat
     4021 caacggtatt ggccattgcc aaaccctatt ggctattgag atttaggcaa gaagcgcagt
     4081 gatttagcag atattggtag gagatgtaga atctgtcttg aagagtcgcc cggatcactg
     4141 cgttacttaa gagcctaatt aacttttaag caccgaggaa agtccggact ccaa
//
LOCUS       DAOROD010000041         3590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_2638_length_3590_cov_35.766054, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000041 DAOROD010000000
VERSION     DAOROD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_3590_cov_35.766054"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLW53_05815"
     CDS             complement(<1..692)
                     /locus_tag="PLW53_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD36970.1"
                     /translation="MTLKTDLYIDGKWVKGSGTVPVYDPSDGSVIAEIQTAGEKECDA
                     AVEAAHRAFPEWAKTAPRYRAEILRKAFEIMVSEADQLAELVSRENGKVVSDAKGEIL
                     YAAEFFRWFSEETVRVAGDFRKSPSGDKRILVTHQPIGVSLLITPWNFPAGMATRKIG
                     PAIGAGCTVILKPAIETPLTAMAIVDILERAGVPKGVVNLITPTPAGPMVSRMLHDPR
                     VMNLSFTGSTEVG"
     gene            742..3084
                     /locus_tag="PLW53_05820"
     CDS             742..3084
                     /locus_tag="PLW53_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRD36971.1"
                     /translation="MAKSWTDGAPSPKALQEHAHLRDPFTYRLKKFFLGAPLNRHTLS
                     HQRLKKRYALGILSSDCISSSAYGSEQILIALLPAFGLAAFTLLMPMTFVVLTVLLII
                     TLSYRNVISVYTQFGGAYIVSRDNFGPVVAQIAAVALILDYIVTLAIQTAAGVAAIIS
                     TFPELAPWKIHMCFAVIALLSFGNLRGVKEAGRAFALPTYLFVGSMFTVFAIGIYREL
                     TTGLPRLDPSVEGAIEIGSEQGLLSAAAIFILLRAFANGGSSLTGLEAIADGVGLFKS
                     PESVNAKKTLVIMSLILGSLVLGVSYFAHRILAVPYQDETPTVISQVASTILGDGTFG
                     SIFFIIVQAATMLILFAGANTTFSAFPIVVNYAAADGYLPKWLTKRGHKLNFSNGILV
                     LSSAALILVLVTAGSVSYLVAFYALGVFTAFTLSGLGMAKYNYVNREGSWKLKFAINA
                     IAGGLSLIIVIIFSIVKFTEGAWVVLIVAPILVTAFLRLRKRYESEQSALLVRKEQER
                     ALSMTRHDVTVLVDSVDLATVGTVRYARSLNPRSIHAVHFVLDDRRAEVIRKEWMSNH
                     GVDDVPIEFIDCPDRRLPNSAVDYAIRATQNPEVELTLLLPRRAYRGFLGRLLHDQTA
                     EEIARPISQLPRVVATIVPFDIDKIISGQAQIAPKVEEKVAPKAKVETPIRAQSNWLQ
                     SSEVVSHYQESMMPIGNIIWRKRAHVSGKVTAIRPAAMDSAPTLEVEIWDESGGVTLQ
                     FLGRREIAGLEVGSTLIAEGMVGEHDGSLTILNPSYEIRV"
     gene            3087..3482
                     /locus_tag="PLW53_05825"
     CDS             3087..3482
                     /locus_tag="PLW53_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRD36972.1"
                     /translation="MIKRLLSKSSARWFIVGSATFLIDTALFLLAFSLTDIVIASNLF
                     SGAIATLFNYLSHYHWSFASDREHKQSTLIYLTFFFIFLFLGTTLVTTLVDSGLEPAF
                     AKVGSALFTAPISFFIMKFVTFRRRSDAK"
BASE COUNT          933 a          780 c          766 g         1111 t
ORIGIN      
        1 ccaacctctg ttgatccagt aaatgaaaga ttcataactc ttggatcatg gagcattcga
       61 ctaaccattg gccctgctgg cgttggagta attagattga ctactccctt tggcactcca
      121 gctcgctcta aaatatcaac aattgccatc gctgttagcg gtgtttcaat tgcaggtttg
      181 agaattacag tacaacctgc cccaattgct gggccaattt ttctagttgc cattcctgct
      241 ggaaaattcc aaggagtaat aagaagtgag actccgattg gttgatgggt tactaatatt
      301 cttttatctc cacttggcga ctttctaaaa tcgcctgcaa ctcttaccgt ctcctcgcta
      361 aaccaacgga agaactcagc ggcatataaa atctcgccct ttgcatcgct aacaactttt
      421 ccattctctc ttgaaacaag ctctgctaat tgatcagcct cagaaaccat aatttcaaat
      481 gccttgcgca gaatctcagc tcgatatctt ggagcagttt ttgcccactc tggaaatgct
      541 ctatgagcag cttcaactgc agcgtcgcac tctttctcac cagcggtttg aatctctgca
      601 attacgctgc catcacttgg gtcatagacg ggaacagttc ctgagccttt aacccacttg
      661 ccatcgatat agagatcggt ttttagcgtc ataggctcag agcatacgcg tgaaaagggg
      721 ggcgtagaat gggcaactac cgtggctaaa tcctggacag atggtgcgcc gagccccaag
      781 gcgcttcaag aacatgcgca cttgagggac cctttcacat accgtttaaa gaagtttttc
      841 cttggcgctc cactaaatcg ccacacttta agtcatcaaa gattaaaaaa gcgctatgcc
      901 cttggaattc tatcctcgga ctgtatttca tcttcggcat atggctctga gcaaatttta
      961 attgcgcttc ttccagcctt tggcttggct gcatttacct tgctgatgcc tatgactttt
     1021 gtagtgttga cagtgctttt aattattacg ctctcatacc gaaacgtaat ttcggtttat
     1081 acccaatttg gtggcgctta tattgtctca agagataatt ttggaccagt agttgctcag
     1141 attgcggcag ttgctctaat ccttgattac atagtgactc ttgctattca aactgctgca
     1201 ggagttgcag caattatttc aacatttcct gaactagctc cttggaagat tcatatgtgc
     1261 tttgcggtga tagcacttct ctcttttgga aacttaagag gcgttaaaga agcaggaagg
     1321 gcatttgcgc ttcctaccta tttattcgtg ggttctatgt ttaccgtctt tgcaattgga
     1381 atctatcggg aattgaccac cgggctacca aggcttgatc cgagcgttga aggagctatc
     1441 gaaattggca gcgagcaagg cctgctctct gcggctgcaa tatttattct tcttagagca
     1501 tttgcaaatg gcggctcttc tctaactgga cttgaggcta ttgctgatgg agttggcctc
     1561 tttaagagcc ctgaatcggt aaatgccaaa aagactctgg taattatgag cttaattttg
     1621 ggttcattag tacttggcgt ctcttatttt gcgcaccgaa ttttggcagt gccatatcaa
     1681 gatgaaactc cgaccgtaat ttcccaggtt gcctcaacca ttttgggaga tggcactttt
     1741 ggctcaatct tcttcataat tgtgcaagca gcaacaatgt taattctctt tgctggagca
     1801 aacacaacat ttagcgcgtt tccaattgta gttaattacg ctgctgctga tggctatcta
     1861 ccaaaatggc taacaaagcg tggacataaa cttaatttct ctaatgggat tttggtttta
     1921 tcatctgcag ctttgatctt ggtgcttgtt actgctggtt cggtctctta cctagttgca
     1981 ttttatgcct taggtgtttt caccgctttc actctctctg gtcttggaat ggctaaatat
     2041 aactacgtca atagagaggg ttcctggaaa ttaaagtttg ccatcaatgc aatcgctggt
     2101 ggactttcgc tgatcattgt aattattttt tcaatagtta aattcactga aggtgcatgg
     2161 gtggtactaa ttgttgctcc aattcttgtc actgcatttc tccggttaag aaaacgatat
     2221 gaatcagagc aaagtgcttt gctggttaga aaagagcagg agcgagctct ttctatgact
     2281 cgacatgatg tcaccgtatt ggttgattcc gtcgatctag caacggtagg gacagtgcgg
     2341 tatgcccgtt cattaaatcc aaggtcaata catgctgttc actttgtctt agatgaccgt
     2401 cgcgctgagg taattcgaaa agaatggatg agtaatcacg gagtagatga tgttccaatt
     2461 gaatttatag attgcccaga tcgaagattg ccaaattcag cggttgatta tgcaattcgt
     2521 gccacacaga atcctgaagt ggaattaaca cttcttcttc ctagacgtgc atatcggggt
     2581 tttctaggtc gattgctaca cgatcaaaca gcagaagaga ttgctcgccc aatctcacaa
     2641 cttcctagag tagttgcaac cattgttccc tttgacatcg acaaaataat ctctgggcag
     2701 gcacaaattg cgccaaaggt tgaagagaag gtcgcgccaa aggccaaagt agagacaccg
     2761 attcgagctc aaagtaattg gctccaatca tctgaggtag ttagccacta tcaagaatcc
     2821 atgatgccga ttggaaacat tatttggcgc aaaagagccc atgtttctgg aaaagtaact
     2881 gcaattagac ctgctgctat ggatagcgcg ccaactttag aagttgagat ctgggatgaa
     2941 agcggcggag ttaccctgca atttctagga agacgagaga tagcaggact tgaagttggc
     3001 tcaacactta ttgcagaggg tatggtcggt gaacatgatg gctcactgac tattcttaat
     3061 ccttcatatg agataagggt ttagctttga ttaaaagatt gctaagcaaa agcagcgcta
     3121 gatggttcat tgttggaagc gcgacatttc taattgatac agctctcttt ctccttgcgt
     3181 tttctctaac agatatcgtt attgcatcaa atttattctc cggagcgatt gcaactctat
     3241 ttaactatct atctcactat cactggtcct ttgctagtga tagggagcat aagcagtcaa
     3301 ctttgatata cctcaccttt ttcttcatat tcctatttct gggaacaacg cttgtaacta
     3361 ctctggttga ctctggtcta gaacctgcct ttgcaaaggt aggtagcgca ctatttactg
     3421 ctccgattag tttctttata atgaagtttg taacctttag acgacgcagc gatgcgaaat
     3481 agattaataa ttgttcctac ctataacgag ttggctaatg ctcctttact aattaggcgt
     3541 atctttaaac atattccaaa ctctgatgtc ttagtagttg atgatggatc
//
LOCUS       DAOROD010000042         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_3301_length_3177_cov_32.641256, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000042 DAOROD010000000
VERSION     DAOROD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_3177_cov_32.641256"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLW53_05830"
     CDS             complement(<1..242)
                     /locus_tag="PLW53_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36973.1"
                     /translation="MSRKPSLAVVGATGAVGTVMLDILTKRENVWGEIRLIASERSAG
                     KKLLCRGEELTVVALSPEAFDGIDVAMFDVPDEISA"
     gene            complement(239..1510)
                     /locus_tag="PLW53_05835"
     CDS             complement(239..1510)
                     /locus_tag="PLW53_05835"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005869671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRD36974.1"
                     /translation="MGLIVQKFGGSSVADAESIKRVAARIAASKRSGQQVVVVVSAMG
                     DTTDELIDLANQVSPMPNGRELDMLLTAGERISMALLAMAISNLGLEARSFTGSQAGI
                     ITTSTHGRARVIDVTPGRIQEALAEGAIAIVAGFQGVAQDTKDITTLGRGGSDTTAVA
                     LAAALDADVCEIYTDVDGVFSADPRIVPTARKLKSVTYEEMLELAASGAKVLHLRCVE
                     YARRFNLPIHVRSSFSNLEGTWVVKDQPEGGSMEQAIISGIAHDKSEAKITIVGVPDR
                     AGVAARIFQAIADADINIDMIVQNVSAAATGLTDISFTLPRTEGSRGTSIVQKLQGEI
                     GFQSIQYDDQIGKLSLIGAGMRSHPGVTANFFAAMASAGVNIEMISTSEIRISIVCRQ
                     ADIERAVQAAHTAFDLDADQIEAVVYGGSGI"
     gene            complement(1583..2182)
                     /gene="recR"
                     /locus_tag="PLW53_05840"
     CDS             complement(1583..2182)
                     /gene="recR"
                     /locus_tag="PLW53_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRD36975.1"
                     /translation="MYEGIIQELIDELGRLPGIGPKSAQRIAFHVIQSESVDVNRLVE
                     VLRLVKERVKFCSQCFNIAEEELCKICRDVRRDDSLICVVEESKDVVAIERTREFRGK
                     YHVLGGAISPIDGIGPEQLKIRELLTRLADDRIKEIIIATDPNLEGEATATYLARTMK
                     PLGIKISRLASGLPVGGDLEYADEVTLGRAFEGRRSLDL"
     gene            complement(2182..>3177)
                     /locus_tag="PLW53_05845"
     CDS             complement(2182..>3177)
                     /locus_tag="PLW53_05845"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36976.1"
                     /translation="KDGVTYEIAVQLLGYTDGALLDDAIDAIGARDTATLFKTIDLLV
                     ESGHDPRRFAFDLLERLRDLVIVDALADQAATVLRGYPQDQLTRMASQASRIGSASLN
                     RAAALLAEGLTAMRGATPARLILELVAGKITIAQGDSSDTGLISRLERLERQSGFDVA
                     NTPAAAPTQKANKTEGKAEPKAIKEEPKQAKSEPVKSEKAPSSADIAALRRMWPDVIE
                     SVKRRRRLTWSLISASAQILSVDEKQITIGIVNAGARDSFIRSESEQILIDAFDEVTG
                     IRRKIEVVVDPSTSANSNEAKSARVDEDPDDEVRLSGTELLMKELGARVIGESDK"
BASE COUNT          820 a          793 c          652 g          912 t
ORIGIN      
        1 atggcagaga tttcatctgg aacatcaaac atagcaacat caattccatc aaatgcctca
       61 ggagagagcg caacaacagt tagctcttct ccgcggcaga gtaatttctt tccagcgctt
      121 cgctcagatg caattagtcg aatctcgccc cagacatttt ctcgtttggt gagaatatcg
      181 agcatcacag ttccaactgc tcctgttgct ccaactactg caagtgatgg ctttctactc
      241 atatgccgct tccgccatag acaacagctt caatctgatc agcatctaaa tcaaatgcgg
      301 tatgagctgc ttgaactgct cgctcaatat ctgcctggcg acagacgata gagatgcgaa
      361 tttcagaagt tgaaatcatt tcaatattaa ctccagcaga ggccatagca gcaaagaagt
      421 tagctgttac tccaggatgt gagcgcatac cagcgccaat taaggataat ttgccgatct
      481 gatcatcata ttgaatcgat tgaaaaccaa tctctccctg tagtttttga actattgatg
      541 ttcctcttga accttcagtt cttggcaggg taaatgaaat atcggtaaga cctgtggctg
      601 ctgcggagac attttgaaca atcatgtcga tattgatatc ggcatctgcg attgcctgaa
      661 agattcgagc tgctactcct gctctatctg gcaccccaac tatggtgatc ttggcttcac
      721 tcttatcatg agcgattcca gagatgattg cttgctccat gcttcctcct tcaggttggt
      781 ctttaacgac ccaggttcct tctaaattgg aaaatgatga acggacatgt attggtagat
      841 taaatcgtct agcatattca acgcagcgaa ggtgtaatac ttttgcaccg gatgctgcaa
      901 gttctaacat ctcttcatag gtgacgctct ttagctttct tgcagttgga acaattcttg
      961 gatctgcgct aaatactcca tcaacatctg tgtaaatctc acagacatca gcatcaagtg
     1021 cggcagcaag agccacagct gttgtatcag agccgcctct gccaagtgtt gtaatatctt
     1081 ttgtatcttg agcaactcct tgaaaacctg caacgattgc aattgctccc tctgctagag
     1141 cctcttgaat tcgccctggg gtcacatcaa ttactcgcgc tcttccatga gttgaagttg
     1201 tgataattcc tgcttggctt ccagtaaagg agcgagcctc aagaccaaga tttgaaatag
     1261 ccattgcaag aagagccata gagattcgct cgcctgcagt tagaagcatg tctagctcgc
     1321 gtccatttgg cattggagag acttgattag ctaaatcaat tagctcatct gtggtatcgc
     1381 ccatggcaga gacgactaca actacctgct ggccagatct cttggaggca gcgatgcggg
     1441 cagcaacccg tttgatgctc tcagcatcgg caacggagga gcccccgaac ttctggacga
     1501 ttagacccat ggcctcatgt tggcagagag ttaaagcaaa gcggagttta tctcatatat
     1561 caagacgggt tttgtctcac tattagagat ctaaagacct tcgaccttca aaagctcttc
     1621 caagggtgac ttcatcggca tattcaaggt ccccgccaac aggaaggcca gatgcaaggc
     1681 gagagatctt tatcccaagt ggcttcattg ttcttgctag ataggtagct gttgcctcac
     1741 cttcaaggtt tggatcggta gcgatgatga tctctttaat ccgatcatcg gcaaggcgag
     1801 tgagaagctc tctgattttt agctgctcag gaccaatccc atctatcggg ctaattgccc
     1861 ctcctagaac atgatactta ccgcgaaatt ctctagttct ctcaatagcg acaacatctt
     1921 tgctctcttc aacaacacag atcagagaat catctcgcct tacatcgcgg cagatcttac
     1981 agagctcttc ttctgcgata ttaaaacatt ggctacagaa cttaactcgc tctttgacta
     2041 ggcgaaggac ttcgactaaa cgattgacat caacgctctc tgattgaata acatgaaagg
     2101 caattctctg ggcgctcttt gggccaattc ctggcaggcg acctagctca tcaattagct
     2161 cttgaataat tccttcgtac atcacttatc actctcgcca ataacgcgcg cgccaagttc
     2221 tttcatcaat agctctgttc cagagaggcg aacttcatca tctggatctt catcaactct
     2281 tgcactcttt gcctcatttg agttagcaga agttgatgga tctaccacca cttcaatctt
     2341 tcgcctaatg cctgttactt catcaaaggc atcaattaga atctgctcgc tctcagatct
     2401 aataaatgaa tcccttgctc cagcattaac aattccaatt gtaatttgct tctcatccac
     2461 acttagaatt tgagcacttg ctgaaataag cgaccaggtg agtcttcgtc ttctcttaac
     2521 gctctcaata acatctggcc acatccgcct taaagctgca atatcagcgc tacttggagc
     2581 cttctcgctc tttaccggtt cactttttgc ttgcttaggc tcttctttaa ttgcttttgg
     2641 ctcagctttt ccttcagttt tattagcctt ttgagtaggg gcagcagcag gggtgtttgc
     2701 aacatcaaat cctgattggc gttcaagtcg ctcaagcctt gagataagac cagtatcact
     2761 tgaatcacct tgagcaattg ttatcttccc tgcaactaac tccagaatca gccttgcagg
     2821 cgttgctcct cgcatcgctg ttaacccttc agcaagcaga gctgctgccc tatttagtga
     2881 tgcgctgcca attcttgatg cctgtgatgc cattcttgtt agttgatctt gaggatagcc
     2941 gcgaagaacg gtagctgctt gatcagcaag ggcatccaca ataactagat ctcttaagcg
     3001 ctcaagtaaa tcaaaggcaa agcgccttgg gtcatggcca gattcaacaa gtagatcgat
     3061 tgttttaaat aacgttgcgg tgtctcttgc acctattgca tcaatagcat catctaagag
     3121 cgcgccatcg gtatagccaa gtagttgaac tgcaatctca taggtaacgc catcttt
//
LOCUS       DAOROD010000043         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_5085_length_2546_cov_26.468888, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000043 DAOROD010000000
VERSION     DAOROD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_2546_cov_26.468888"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PLW53_05850"
     CDS             complement(<1..1497)
                     /locus_tag="PLW53_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36977.1"
                     /translation="MKKRSKGAVLSVVFTLLLSLFIPTSYSAQKITAGATCKGLNKKV
                     DYKNKTYTCIKKGNKLVWSKGVAIKSATPATSPSPTPAASAPAAEAKVDSLSLDPRIS
                     KDSLLSPASICKTIDKTPDYAPFGVTISRNGFPRPKEATYPSGKTKVLVIPFTYTTYP
                     FITKLPPITNRTVTDLDAIKKVNKEVEELVKRLSAGKFEMEISILPESEWLTLDPELS
                     FDSTPMTDNFGPIKKMIQKNDGKIDFEKYDSYVFVTTLGAPIPGVAQATYSTDVKTSK
                     GQANKLVLMSQGWSSSSLYFHELGHSMFGLEDLYLFSENKAEWLPSELTPILSWDLMA
                     NAQLETLSNWNRLLMGWITDSEIRCLTDQSQSTHYLSDFTKNGQPQLLLINLAPGVSL
                     AAESRIWGETQRLLLYVIDTNISHGQGPLRSLNTLLKAGESKELFDWKFNVLETSKDG
                     LLLEVSKGSGKAYVAPVIKQNNQGPRQPDSPIGLTGGEFTRSSATSAEI"
     gene            complement(1506..2105)
                     /locus_tag="PLW53_05855"
     CDS             complement(1506..2105)
                     /locus_tag="PLW53_05855"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRD36978.1"
                     /translation="MAFTLPPLPYSYDALEPHIDEATMKLHHGKHHQAYVDKLNAAIA
                     ASEWESKSIDEIMVSLDKVPDKIKAAVRNNGGGHYNHSLFWQWMSPTGGGEPTGKVAE
                     AINAKFGSFAKFKEEFEAAGMSRFGSGWVWLIQDGGELSITTTPYQDCPVSEGKKVLL
                     GNDLWEHAYYLKYQNRRPEYLAAWWNVVNWNKVTELFNQ"
     gene            complement(2185..>2546)
                     /locus_tag="PLW53_05860"
     CDS             complement(2185..>2546)
                     /locus_tag="PLW53_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD36979.1"
                     /translation="INSWNQQRAIRVKSQLAPTVLLSAVLALCATGAITSDSDQYLKL
                     FLVGLVASGGVFSVTAMVAAVRDSLYVIDSLKALKSVSALSSSIIKSGSQLKALALLF
                     MAMSTFNFVALLLYLYS"
BASE COUNT          701 a          513 c          553 g          779 t
ORIGIN      
        1 aatctcagca cttgttgcac tacttctagt gaattcacca ccagttaatc caatgggtga
       61 gtctggttgt ctaggtcctt ggttattttg cttaattact ggagctacat aggccttgcc
      121 agatccttta ctaacttcaa gtaatagacc atctttactt gtctctagaa cgttaaattt
      181 ccaatcaaat aactcttttg actcaccagc ttttagaaga gtattaagtg atctaagtgg
      241 tccttggcca tggctgatgt tggtatcaat tacatataaa aggagtcttt gggtctctcc
      301 ccagattctt gattcagctg ccaggctcac tcctggagca agattgataa ggagtaattg
      361 aggttggcca ttctttgtga agtcagagag atagtgagtg gattgacttt gatcagtaag
      421 gcagcgaatc tctgaatcag taatccatcc catcaatagg cgattccagt tagagagagt
      481 ttctaactga gcattggcca tcagatccca actcaatata ggtgtgagct cagatggcaa
      541 ccactctgcc ttattttcac taaagagata aagatcctct agaccaaaca tagagtggcc
      601 cagttcatgg aaatacagtg atgagctgga ccagccttga gacattaaga cgagtttatt
      661 tgcttgaccc ttactggttt ttacatcggt tgagtaagta gcttgagcaa ctcctggaat
      721 aggcgcaccg agagtagtta caaagacata agaatcgtat ttttcaaaat caatcttgcc
      781 gtcatttttt tggatcatct tcttgattgg tccaaagtta tctgtcattg gtgttgaatc
      841 aaagctcaat tctggatcaa gagttagcca ttcactctct ggaagtattg atatttccat
      901 ttcaaatttg ccagcagata ggcgctttac tagctcctcg acctctttgt ttactttttt
      961 gatcgcatca agatctgtca ccgttctatt ggtgataggt ggcaatttgg taatgaaggg
     1021 atatgttgta taggtgaaag gaattacaag aacctttgtc ttgccagagg gataggtcgc
     1081 ctctttaggg cgaggaaatc cattgcgaga tattgttact ccgaaaggag cataatcagg
     1141 ggtcttatct attgttttgc agattgaagc tggcgaaaga agtgaatctt tggaaattct
     1201 tggatccaaa cttaaagaat ctacttttgc ctctgcagca ggagcgcttg ccgcaggagt
     1261 tggtgatgga gaagtggcag gggtcgcact ttttatggca acgccctttg accaaactaa
     1321 cttattgccc tttttgatgc aggtataggt tttgttcttg taatcaactt tcttgtttaa
     1381 gcccttacaa gtggcgcccg ctgtaatctt ttgagcagag taagaggtgg gaataaataa
     1441 ggagagaagt aaagtaaaga cgactgataa aactgcgcct ttacttcttt tcttcataca
     1501 cccagttatt gattaaagag ctctgtaacc ttgttccaat taactacgtt ccaccatgct
     1561 gcaaggtatt ccggtcttct attctggtat ttgaggtaat aagcatgctc ccagagatca
     1621 tttcctagaa gtactttctt tccctcactt actgggcaat cttgatatgg ggtggtggtt
     1681 attgaaagct cgccgccatc ttgaattaac cagacccaac cagagccaaa gcgactcatt
     1741 cctgctgctt caaactcttc cttaaacttt gcaaaggatc caaacttggc atttattgcc
     1801 tctgctactt tgccagttgg ctcaccgccg ccagttgggc tcatccactg ccaaaacagg
     1861 gagtggttgt aatggccgcc gccattattt ctaaccgctg ctttaatttt atctggcact
     1921 ttatcaagtg agaccataat ttcatcgatg ctctttgatt cccattcact tgctgcaatc
     1981 gctgcattta atttatcaac ataggcctga tgatgtttgc catgatgaag cttcattgtg
     2041 gcctcatcga tatgaggctc tagagcgtca taggagtaag gaagtggtgg gagtgtgaaa
     2101 gccattttaa ttttcgcctt tcaatgggag taaagtcaag gttagtttag ccctggctgc
     2161 ttaaagagca agatagatca aggcctagct atataaatag agtaggagcg caacaaagtt
     2221 aaaggtgctc atcgccataa agagaagggc aagagccttt agttgagaac ctgatttgat
     2281 tattgatgaa ctaagagctg agacagattt aagagctttt agtgaatcaa tcacataaag
     2341 gctatctcta actgctgcca ccatagctgt tactgagaag actccccctg aggcaacaag
     2401 accaactaaa aagagcttta aatattgatc gctatcgcta gtaatagcac ctgttgcaca
     2461 gagcgctaat actgctgaga gaagaactgt gggagctaat tgagatttga ctctaatagc
     2521 tctttgttga ttccaagaat taatta
//
LOCUS       DAOROD010000044         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_7142_length_2128_cov_36.884226, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000044 DAOROD010000000
VERSION     DAOROD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_2128_cov_36.884226"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLW53_05865"
     CDS             complement(<1..401)
                     /locus_tag="PLW53_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD36980.1"
                     /translation="MKVRQIASTDYDQWLNLWRGYLEFYKSEVSDEITANTWRELNDS
                     KSLITGFVAEIDSTVCGIAHCFLRPSTWHKVGYLYLEDLFVDPKYRGAGVGRALLDKA
                     AEYGREIGAEKLYWITKEDNAQARALYDSFV"
     gene            462..1142
                     /locus_tag="PLW53_05870"
     CDS             462..1142
                     /locus_tag="PLW53_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRD36981.1"
                     /translation="MKSLKAYAIAAHFGPTMLITTISFLLAARLWWEGPAYLIAFTVF
                     LGQLLIGWSNDIYDYQDDLKHNRVNKPLVSGQLQIENLRRATFILLPIALLANLLGPL
                     GLKGGAVYLLGVGCGIAYNFYFKFRITSPLVYFIALAALPASIFYAVDRDPPLWVLAT
                     SSLLGVAFHFANVLKDLSADRDSNIGGLPQRLGKKASLVIIAILLIIVITILLNSPIT
                     STFTSEVI"
     gene            1154..1873
                     /locus_tag="PLW53_05875"
     CDS             1154..1873
                     /locus_tag="PLW53_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRD36982.1"
                     /translation="MAKGSIALVGSGEFLPEMSDLESLLIQDGVRNGKVARFIQIPTA
                     AGKESPERIKYWKELGRAQGQRLGIEVEFLEILSKQDAHNKELVSLIADSALIYLSGG
                     DPHHLAESLIDTPVFDAIYQGWASGSSLAGCSAGAMALSTHIPNFRFSKKSPTPGFNL
                     LPRVRVIPHFDRFFRWIPESAAKVLMSAPDETILIGIDELTALVNRSGEDDWQVIGSG
                     LVHILSGAPSAQLAARQRITL"
     gene            1893..>2128
                     /locus_tag="PLW53_05880"
     CDS             1893..>2128
                     /locus_tag="PLW53_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HRD36983.1"
                     /translation="MSFVMYSTTWCGYCKRLKSQLADLGISFEEINIEEVAGTAEIVE
                     KVNGGNQTVPTLVFSDGSAMTNPSAKQVQEKLASL"
BASE COUNT          613 a          476 c          436 g          603 t
ORIGIN      
        1 tttacaaatg aatcatataa agctctagcc tgagcattat cttccttagt aatccaataa
       61 agcttctcag ctccaatctc tcttccatat tctgcagcct tatcaagaag cgccctgcca
      121 actcctgccc cgcgatactt tggatcaaca aagagatctt ctagatagag atagccaacc
      181 ttgtgccatg tagatggcct gagaaaacaa tgggctatcc cgcaaactgt ggaatcaatt
      241 tctgcaacaa atcctgtaat caaactcttt gaatcattta gttcacgcca agtattagct
      301 gttatttcat cacttacttc agatttatag aactccaagt atccacgcca taaattcagc
      361 cattggtcgt aatcagtgct agcgatctgc ctaaccttca tcacaataga gaatctaacc
      421 ccatatcaaa gaagagccaa cttatatgac actattagga aatgaaatcg ctgaaggcat
      481 atgccatagc cgctcacttc ggcccaacaa tgcttatcac cactatttca ttccttctag
      541 ctgcgcggct ctggtgggag gggccggcct atctaattgc tttcaccgtc tttctaggcc
      601 agcttctaat tggctggagt aatgacatct atgactatca agatgaccta aaacataacc
      661 gagtaaataa acctttagta agtggccaat tacagattga aaatctaaga agggccacat
      721 ttatcctcct tcccattgcg ctgcttgcta acttacttgg accacttggg cttaaaggtg
      781 gcgcggtcta tctactaggt gttggctgcg ggattgccta caacttctat tttaaattcc
      841 gcattacctc tcctctggtt tacttcatcg cccttgcagc gcttccagca agtattttct
      901 atgctgttga tagagatcct ccgctctggg tcttggcaac tagctctctt cttggagttg
      961 catttcactt tgccaatgtt ttaaaggatc taagcgctga tagagatagc aatattggcg
     1021 gccttccgca gcgactaggt aaaaaagcct ctcttgtaat cattgcaata cttcttatta
     1081 ttgttataac gattctgcta aatagcccaa ttacctcaac tttcacctca gaagttattt
     1141 aaggcaaaga tcaatggcta aaggatcaat tgctctagtt ggctctggtg agtttctgcc
     1201 agagatgtca gatcttgaga gccttcttat tcaagatggt gtaagaaatg gcaaagttgc
     1261 aagatttatt caaatcccaa ctgcagcagg caaagagagt ccagagcgca tcaagtattg
     1321 gaaagagcta ggaagagcgc aaggccagcg cttagggata gaggttgaat tcctagaaat
     1381 attaagtaaa caagatgcgc ataataaaga gctggtctca ctgattgcag atagcgctct
     1441 tatctatctc tctggcggag atcctcatca tctagcagaa tcattaattg ataccccagt
     1501 atttgatgcc atctatcaag gctgggctag tggttcatca cttgctggct gcagcgcagg
     1561 tgctatggcg ctttcaactc atattccaaa ctttagattt agtaaaaaga gcccaactcc
     1621 aggatttaat ctcttgccta gagtgagagt aattcctcac tttgatcgct tctttagatg
     1681 gattccagag agcgcagcaa aggtattaat gagcgctcca gatgaaacca tattgatagg
     1741 aattgatgag ctaacagctt tagttaatag aagtggtgag gatgattggc aggtaatagg
     1801 aagcggctta gttcatatct taagcggcgc ccctagcgct caattagcag ctaggcagag
     1861 aattactctc taatatcaat tagagtaaag ccatgtcatt tgttatgtat tcaaccacct
     1921 ggtgtggtta ttgcaagagg ctaaagtcgc aactagcaga ccttggtatc agctttgaag
     1981 agatcaatat cgaagaggtt gccggaacag ctgagattgt tgagaaagta aacgggggaa
     2041 atcaaaccgt tccaacccta gttttctcag atggcagcgc tatgactaac ccatcagcaa
     2101 aacaagtgca agaaaagctt gcttcgct
//
LOCUS       DAOROD010000045         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_8496_length_1942_cov_31.169051, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000045 DAOROD010000000
VERSION     DAOROD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_1942_cov_31.169051"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLW53_05885"
     CDS             complement(<1..545)
                     /locus_tag="PLW53_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD36984.1"
                     /translation="MIKLANTDLTIYPLCLGGNVFGYSADKENSEAVLSFYADNGGNF
                     IDTADMYSQWAPGHIGGESETIIGNWMKKRGNRSQMIIATKVGKLDTKPGLSPSNIIS
                     ACEDSLKRLGSDYIDIYYSHQDDLDTPIEQSLGAYDSLIKAGKVRYIAASNFTPERLQ
                     ESLDISKELNLASYIASQDQY"
     gene            complement(538..1251)
                     /locus_tag="PLW53_05890"
     CDS             complement(538..1251)
                     /locus_tag="PLW53_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD36985.1"
                     /translation="MARLLLLTNSTGASAEVLPALSLLQHNVKILPAVASVLVDIPEV
                     DVLFIDARRDLSAAKSLTKLIKTTGVGCPILLITTEGGLSATSADWGFDDVILDSAGP
                     AEVDARIRVAIGKLEALRIQADPTSGEIKSGDVIIDEKSYTAKIKGRALDLTYKEFEL
                     LKYLAQHPGRVFTRSQLLQEIWGYDYFGGTRTVDVHIRRLRSKLGTEFEAIIGTVRNV
                     GYRFNLSPKDSQLADLAND"
     gene            1272..1682
                     /locus_tag="PLW53_05895"
     CDS             1272..1682
                     /locus_tag="PLW53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD36986.1"
                     /translation="MENYLAIALILTLASIYGLWYQAKSGKIKGSKAKSASLSSEDIG
                     DELGDRVTLLQFSSAFCTPCRATRVLLADITSELDGVKHIDIDAEDNLELVRRLDIRT
                     TPTTLLLDRYGIEVGRAVGAPKRGEVLASLAAIK"
BASE COUNT          533 a          396 c          428 g          585 t
ORIGIN      
        1 ttgtattgat cttgtgatgc gatataggag gcaaggttta gctcctttga aatatctagg
       61 gattcttgca gcctttcggg agtgaaattg gaagctgcga tatatctaac ttttcctgcc
      121 ttaatcaatg agtcatatgc tccaaggctc tgctctattg gagtatctag atcatcttga
      181 tgggagtagt agatatcgat gtaatcactt ccaaggcgct ttagtgaatc ttcgcaggca
      241 gagatgatgt ttgaggggct aagtcctggc ttggtatcaa gctttccaac ctttgtagcg
      301 ataatcattt ggcttctatt gcctcttttt ttcatccaat taccgatgat cgtttctgat
      361 tcaccgccaa tatggcctgg ggcccattgg gagtacatat ctgcagtatc aatgaagttt
      421 ccaccgttat cggcatagaa actaaggacc gcttcagagt tttctttatc tgcgctatag
      481 ccaaagacat tgccaccaag acagagtgga tagatagtta aatcagtatt ggcaagttta
      541 atcattagct agatctgcaa gctgggaatc cttaggcgag agattaaagc gatatcccac
      601 atttctaaca gttccaatga ttgcttcaaa ctctgttcca agctttgatc taagacgtct
      661 gatatggaca tcaacagttc tagtgccacc aaagtagtca tagccccaga tctcttggag
      721 tagttgagat cttgtaaaga ctcttcctgg atgctgggca aggtatttta gaagttcaaa
      781 ttctttatag gttagatcaa gtgcgcgacc tttaatcttt gcggtgtagc tcttctcatc
      841 gattattaca tcaccgcttt taatctcacc tgatgttgga tctgcttgaa ttcgaagcgc
      901 ctctagtttt ccaattgcta ctctgattcg tgcatcaacc tctgctggcc ctgcgctatc
      961 gaggatgaca tcatcaaatc cccaatccgc actcgttgcc gagaggccgc cttctgtagt
     1021 aattaaaaga attgggcagc caactccagt tgtctttatt aatttagtaa gtgatttagc
     1081 tgcgcttaga tctcgcctgg catcaataaa taaaacatca acttctggga tatcaactaa
     1141 tactgaagcc actgctggca ggatttttac gttatgttgc agaaggctta aagcaggcag
     1201 aacttcagcg ctagcgccag tgctattggt taaaagtaga aggcgagcca taagagtgga
     1261 agaatactgg gatggagaat tacctagcaa ttgcacttat tttgacgcta gcaagcatct
     1321 atggcctctg gtatcaggct aaaagcggca agataaaagg ctcaaaggct aagagcgcat
     1381 ctctttcaag tgaagatatt ggagatgagc tgggagacag agtaactctg ctgcaattct
     1441 catcagcatt ctgcacccca tgccgggcaa ctagagtctt gctagctgat atcacctctg
     1501 agcttgatgg ggttaaacat attgatattg atgccgaaga taatttagag ctagtacgcc
     1561 gccttgatat tagaactacc cctacaactc ttcttcttga tagatatggg atcgaggttg
     1621 ggcgagcagt tggcgctcct aagcgtggtg aggtcttagc atcacttgct gctattaagt
     1681 agataagtga aagtaatgca aggtatttgc aagtagcgaa gttccaagac cccttctaca
     1741 ctttggccat ggcttcaata attaaagcgc tctttaccaa aagacgggta attgattttg
     1801 cgcgctttgc tggccagctc tgtctgcagt agttgttgat ttctttttaa caatcaataa
     1861 accaactact agagaattag gaaataattt aaatgtcagt attaagcgtt ttaaagtttg
     1921 aaaaaagtga tagtcaacaa tt
//
LOCUS       DAOROD010000046         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanopelagicaceae bacterium isolate
            AMR_MDS_5430 NODE_11861_length_1637_cov_29.551201, whole genome
            shotgun sequence.
ACCESSION   DAOROD010000046 DAOROD010000000
VERSION     DAOROD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867647
            Sequence Read Archive: SRR1022353
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanopelagicaceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Nanopelagicaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Candidatus
            Nanopelagicales; Candidatus Nanopelagicaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:45:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,181
            CDSs (total)                      :: 1,153
            Genes (coding)                    :: 1,148
            CDSs (with protein)               :: 1,148
            Genes (RNA)                       :: 28
            tRNAs                             :: 27
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Candidatus Nanopelagicaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_1637_cov_29.551201"
                     /isolate="AMR_MDS_5430"
                     /isolation_source="Freshwater fishpond sediment"
                     /db_xref="taxon:2518609"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLW53_05900"
     CDS             complement(<1..1058)
                     /locus_tag="PLW53_05900"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRD36987.1"
                     /translation="MSPDKEIRVAIVGVGNCANSLVQGVHYYRDAARDQEIPGLMNVV
                     VGGYHVGDVKFVAAFDVDKKKVGLDLADAIWASENNTIKFSEVPQSGVKVLRGVTHDG
                     LGRYYRETITESDSPAVDVVKALKDAKVDVLVCYLPVGSEVATKFYAQCAIDAGCGFV
                     NALPVFIASTPEWADKFTKAGLPIVGDDIKSQVGATITHRVLTKLFQDRGVKVDRTYQ
                     LNVGGNMDFKNMLERDRLESKKISKTQSVTSQLDYDMGEGNVHIGPSDYVQWLDDRKW
                     AFVRLEGRAFGDVPLTIEYKLEVWDSPNSAGVIIDALRCAKVAMDRGIGGPLLSPSSY
                     FMKSPPEQYSDEEALVRT"
     gene            complement(1058..1597)
                     /locus_tag="PLW53_05905"
     CDS             complement(1058..1597)
                     /locus_tag="PLW53_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRD36988.1"
                     /translation="MRSRAESLEFALLGLLKESSLHGYELRKRLTAIFGPFRALSFSV
                     LYPQLKRMLDAGLIIQLEVPKGGKSKRVRIVYSITQKGIAKFDELTQGAGELGLDDDN
                     FEVSVAFFGPTSTRNRLRILEGRQRRLKEKAEILKADLDKSAVGLDKYLLEWRRHSLE
                     TAEREIAWLDQMIRSERKN"
BASE COUNT          440 a          410 c          338 g          449 t
ORIGIN      
        1 tttgttctca ctaaagcctc ttcatcagag tactgctcag gaggagactt catgaaatag
       61 ctagatggtg aaagaagtgg cccgccaatt cctctatcca tcgcaacctt tgcgcagcga
      121 agggcatcaa taatgacgcc agctgagttt ggtgaatccc agacctcaag tttgtattca
      181 atagttagtg gaacatcgcc aaatgctcgt ccttcaaggc gaacaaaggc ccacttgcga
      241 tcatcgagcc actgcacata atcagatggc ccaatatgaa cattgccctc tcccatgtcg
      301 taatcaagct gtgatgtaac tgattgagtt ttggagatct tctttgactc taggcgatct
      361 cgctcaagca tgtttttaaa gtccatattt ccaccaacat ttaattgata ggttctatcg
      421 actttgactc cgcggtcttg gaatagcttg gttagaactc tatgggtaat agttgctcca
      481 acttgagatt taatatcatc gccaacgatt ggaaggccgg cttttgtgaa tttatcggcc
      541 cactcagggg ttgaggcgat aaagactgga agtgcgttaa caaaaccgca gccagcatca
      601 attgcgcatt gggcataaaa ctttgtcgca acctctgagc caacaggcag atagcagact
      661 agaacatcaa ccttggcatc cttaagtgct ttaacgacat caacagcggg cgaatcagac
      721 tctgtaattg tctccctgta atagcgacca aggccatcat gagttacacc tcttaaaacc
      781 ttaacgccag attgtggcac ttcagagaat ttgatggtgt tgttttcact agcccagata
      841 gcatctgcaa gatctagacc aacttttttc ttatcaacgt caaaggccgc cacaaacttc
      901 acatcaccta cgtggtatcc accgacgacg acattcatca gacctggaat ctcttgatcc
      961 cttgccgcat ctcggtagta atgcacccct tgcactagag aattggcgca attaccaact
     1021 ccaactattg ctacgcggat ctctttatct gggctcacta gtttttcctt tcagatctga
     1081 tcatttgatc aagccaagca atttctcgct cagcagtttc aagagagtga cggcgccact
     1141 ccagaagata tttatcaaga ccaacagctg acttatctag atcagctttt aagatctcag
     1201 ccttctcctt gaggcggcgc tggcgcccct caagaatcct taatcgattt cgagttgaag
     1261 ttggcccaaa gaatgcgacg ctaacttcaa agttgtcgtc atctaggccg agctctcctg
     1321 ccccttgcgt taattcatcg aatttcgcta tgcctttttg ggttatggag tagacgattc
     1381 gaactctctt ggactttcca cccttgggaa cttccaattg aattattaga cctgcatcaa
     1441 gcattcgctt taattgcgga tagagaactg agaatgaaag agctctaaag gggccaaaaa
     1501 tggctgtaag cctcttgcgg agctcataac catgaaggga gctctccttt aataggccaa
     1561 gaagggcaaa ctcgagcgat tcagctctag atctcatcta ccccaccccc tatttatatc
     1621 gattcgatat atcgagt
//
